<?xml version="1.0" encoding="UTF-8"?>
<uniprot xmlns="https://uniprot.org/uniprot"
 xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"
 xsi:schemaLocation="http://uniprot.org/uniprot http://www.uniprot.org/docs/uniprot.xsd">
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RWS1</accession>
    <name>A0A8S1RWS1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0010338</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RWS1"/>
    <dbReference type="OrthoDB" id="153872at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010402">
        <property type="entry name" value="CCT_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045281">
        <property type="entry name" value="CONSTANS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31319">
        <property type="entry name" value="ZINC FINGER PROTEIN CONSTANS-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31319:SF77">
        <property type="entry name" value="ZINC FINGER PROTEIN CONSTANS-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06203">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51017">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCT" evidence="3">
        <location>
            <begin position="177"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8131892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="245" mass="28626" checksum="59F834EC5CB59B0F" modified="2022-10-12" version="1">MSLGFKLYNTNTNNEVINKRRKLSIGSEGSESTGEKLNESFNRLTLSGGNPSSSFQPYVKIAITQVQQPERTLPQPQLMMGIQKYLKLGEELDVSDILTKPLNDLQQIHNNLTQTQQKFHFIPFGMPANYEKLMAEAQQVLKTEIRVEEERQKRSIDDYLIEQKLLYPGQKLSKEERAAKVAAYLEKKKNRQWKQVKYTVRKNLAEFRQREQGRFTKTDKPRFNQSLIMLDKEERKMETLTNSYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S033</accession>
    <name>A0A8S1S033_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0010439</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S033"/>
    <dbReference type="OrthoDB" id="292337at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002895">
        <property type="entry name" value="Paramecium_SA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01508">
        <property type="entry name" value="Paramecium_SA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035942309" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="17"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8132094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="268" mass="30399" checksum="B25CA2009CA4E940" modified="2022-10-12" version="1">MIQFIIPLTFLIYTTAIVVNQKNQCLCTDYKNQYECALNQQCIWNTTNCIKKSCGDFYYPLQCKNKKECFYYAQNSSCLDLTNCSSLNSTSTSQCESQSSYCGMYNSTAKECYSLTTNPCSQYTTQSECLYSGQGQLCLWENTQCQDFNCTLLDSQTQCQTYNLYCTWILNNQTCVSATCDNKPTSECTLFLSRNNNSTYIQPCYVDYSEKPAKCRDASLSDLSPFTCSLNTLNFATWNNVELDSGSCELCYAPLIQIISLAILIMIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S0R5</accession>
    <name>A0A8S1S0R5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0030026</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8133888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S0R5"/>
    <dbReference type="OrthoDB" id="285654at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8133888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="192" mass="23522" checksum="BA5259B19558561D" modified="2022-10-12" version="1">MLYIQKHNFTNIILKLPNENPFLIGIGYPLNYLDCQDKFYKYLQLHKFKKIPIFQRIEEFNYTQPINKLPLCFDTNMLPYFKQLNDTLYKKNRYKLGNFDYRCELNEEIINSFCQEFYNEIHSHIIYNFITLIQQLKQKYNYEIKDVIVNKKPDDHISKLFNQYLNLNLILMSEMPTYKQLVYIPGFIYFEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S2G9</accession>
    <name>A0A8S1S2G9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0040313</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S2G9"/>
    <dbReference type="OrthoDB" id="1927454at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34894:SF5">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34894">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE RSMI, CONSERVED SITE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="1097"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="374"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="631"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="707"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="804"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="303"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="639"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="657"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="707"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="757"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="804"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="847"/>
            <end position="859"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8135611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1280" mass="151896" checksum="E790F62450855D98" modified="2022-10-12" version="1">MNNTNCFPKIQKGQSTAKLFIKTDWDQSINSSIPLTIRAEMSSNRMKTDFTNKNFQTNSDVSYLKNKHKKFQSIESSIYKDPELSQTKYDKSLQSFYSTMYRKSQNKLQFNNKIDKTINKGQWLDFEELLKDKNLMENGLQKYFFDILKHEIKQSPNQSVIPPNRQQALDLKEWLFFMLQRTKDKSKNLTSIQIAEEIQLIYTSCFKEIIKQVKVECVDRGELLQIIWDFYIQLIDQIIKSSNQQMQCVEQEMKNKMIEQQNFYNEQLHYQELIYNESQNQIKQLQFDYDRALDVDQQQKQEILQLTQEYREALEVIEQHRKQIDELQLQIVMLKTEQINDNNPENEKINIKVQQGQSIVSHLMNFQAKIDNNQKYRIKSPFKSQGSKSPGRSPKKNSIMFDRWEQLRIQENQLKSQIISLKYLDFDKDQQPSQNKIIQISGTDDKSVQTEDQFLNQNNNQQEQHNLQSQQNISILEKSKLVSNSLEQKISMAMKMLEEQKLDENNKQLLSRALSESKEVCEQNKSLSMRLISMNRLDSMQKMELLEKEELCQELEMKLQQTVQDFISIIQNSKKKLYKLVTKNTQLIGTLKQIESKYEIKINDVIDEEECQINNYLNDIDQNKEDNELSLSKESQNLDDERYPQEKSIKMVDSINLKKQTNQKSQNYQEDNSETINNDQSEQFDNYFDNQTSPKDIRYLQDIYHSQKESQHTEPNDENTFKDSPDQMPSDHSKSVLLINPNQNNKSKELDENLIQKNNDLKTSTKNKTNSKNNPTNSKSIQRIQQQQLGDSKIINQQQYHIRNSKNQLDNNSIKPTKNNSKHNISNSPIRETDEISQINQSQRSLVESDRMDLDDEKNRSQRIKDVISQYKDKQFTKYSSAHKTLNYNLLQETKQAKKFRRVYSQNTDVANNLLKQVMNSKNLTKTLSFLQFNKIINQILTDVSKQNEAYKIPFHVCIYDFIKNKYGFKQVAEKKIKQIYEFIIIEKEKNAKVLLISKYCNLKNEIDEVAQKLLIEAFLFFNNKIESNKTEFFITYESANEFLNEKSQLWLQTNQMQQLQNQFKVLNQQYGSNKHYINFDQFIMKILDFYIFNKKDYQKILETLFKAADLDGNKLIEYQEFKTLYRAIHTQQMLNESLLQVFLKNADFADDQGDKYITLPRFTEMAIELSIFPKESVIKYGEGSEKIKEHWENEKNTIKSRFLEAKQYSKVKHTFVELDNLINNNKKEIQNILWMSYKLLNEQSLRVYLKYQTKQCLSELLPLEILEIQQQYKQLDTIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S2T0</accession>
    <name>A0A8S1S2T0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0040374</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S2T0"/>
    <dbReference type="OrthoDB" id="311264at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000950">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050907">
        <property type="entry name" value="SRSF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23147">
        <property type="entry name" value="SERINE/ARGININE RICH SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="17"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="5">
        <location>
            <begin position="97"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="105"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="115"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="127"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="140"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="190"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8135711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="219" mass="24547" checksum="7C2920CC83AB7C1B" modified="2022-10-12" version="1">MSSSSSSRSRSPRKNKKRVYVTGYSLKEDQHDIKKLFKKFGKIEEFAWKGKYCFIEFKDPEDAEKAVKKMNKEEVKGSVLQVEMARGNKPSKNNGLCYSCGHSGHFAKNCRRRSSSSSSSSSSSESRRKHKKKHKKKKSSSSSDSSSSSSSRDKKKKLNKKRKSSKRKSKSSSRGSSKDSHNSESSGSVEGDKQESNNIKEDKQPKDEENQGKAGDGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S4N0</accession>
    <name>A0A8S1S4N0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0030184</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S4N0"/>
    <dbReference type="OrthoDB" id="284018at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001773">
        <property type="entry name" value="Small GTP-binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8134200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="210" mass="23957" checksum="D49655077D801DD5" modified="2022-10-12" version="1">MNETLKLVLVGQLGAGKTSLLQSHRQQEFRAHNAPTVAVDFAGVKNVEVNGRLFDISIWDTAGQEKFRSITRMSLQNTDVAILCFDLSDPDSYRHLQGWIDFLQVNCRSEMGIIIVGTKMDLPTFYNQEDLQKFLQTLNSQQQQLKLFLTSAKTHEGIEETFKYAYEQGAKIKMKALQMDKSILLVPPEQSNNKKRQPSKNKYLQGCYCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SBD7</accession>
    <name>A0A8S1SBD7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0050367</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SBD7"/>
    <dbReference type="OrthoDB" id="304523at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8136830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="660" mass="78303" checksum="16A178422F600EF4" modified="2022-10-12" version="1">MKEGLYSFKQHIDQTKNQRAPTDKRSIQRDLINLQKNGFVYLDKIQEKKIDLLDLDKCLQKLNPHEHKQSESLNKHILQVQILGGLKQQEDLNKLEEFIQQKQNKQYEEQLNQLTNANQFQQKHHLFFDGKRRAEDINLVEEKAYVRTHRLGALLSCLKSNPKYKPDEQQQQLDEQQQIIQQDELSIQQQITKKSSKQNDKTIPQLIAERQEQEPELSKEFLTVFKNDTTLDKSKLQAAFPRIDFDNVKLEKQFDLSKIQTIEKSKDSTLAPLKINKVQFNPVAKQITYDVDSYEENGESVFKKIEDFHESLRKGQSQDTIIDKKIEIVTEAEKQEYKWSQNQYDYYDKIKKLYIDKQTPQNYQQQIEEDQHYNQVNKNPKFNNIYLNTTIKRDNNYQKASNQSQSIVDSISKLQNLKFHQYETAKDLQLEDRPTKDQSQIEVLQQQLQKVQESYFDKDLDDQEVFNSYNSDINRLNQVIDNKGNDIFAGVETLYRQYNSNQFDKSLQQLAPNFNGVVKLDFEKVKEQFKVIPTKQKQYNEQQKGQFSQFYQNIQGPLDLLHQINNTQQQAQEIEQDLIHNELHQKSLYLIGQLPQPQFFANKYTTFKNGNEKSVFNLIGNQHQIIKTPDKDLQKRYRYQNILSNVDDARERFKYNLTCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SCG0</accession>
    <name>A0A8S1SCG0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0050178</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SCG0"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF274">
        <property type="entry name" value="MYB-LIKE DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="57"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="57"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8136494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="461" mass="54136" checksum="056B9068DDECE409" modified="2022-10-12" version="1">MTDRKAWNQKEDMAILQLVKQYGIKKWTIVAEKMKEVYGLFGRSGKQCRERYHNHLDPTINKEPWSENEEKVIFVAHKEHGNKWAEIAKLLPGRTDNAIKNHFYSTLRRSLRRINKLIGDKNKKGTQQIKDIKPGVLSKIFILAEKNPSELKDDHMKKLCQACKGLQDSILEFAQSKQKSHINQFNEDKFKQLIDKIMEFNTLYTKQRESRLKLKKKNHKKRKSRIDDDDDDDDYTSDYKQQEISNQVPLKRSSRLNAKKKVHIDKEDYIDICIRTKKGPLFNIIRDEFEIQQDNEEQQSSNNYNQQYIYDYQGNNSPNNQQMTPAFPVLTPRQIFFQKPTNSYLEEQGLHDDNPLSKSSFVPIVITKPYTQCREKTLDTIAQQLQQKINANADKYIFNQQTTQDSDLEINIGDAFEVQKDYKSPTNKFGIGGYSPSAFRKYKKDQETGLVNFMVTPHNYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SEN5</accession>
    <name>A0A8S1SEN5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0070236</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SEN5"/>
    <dbReference type="OrthoDB" id="431119at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000157">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000101">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF9">
        <property type="entry name" value="GTPASE ACTIVATING PROTEIN AND CENTROSOME-ASSOCIATED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="90"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="461"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8138653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="493" mass="58613" checksum="52BB6D2931C3299D" modified="2022-10-12" version="1">MNKKYTSSNLNPKILDDRDDINFGTSFIDIIMNQSTFNHLTSFVDTGSLINFQLSSKVFKIRRWKKYFKNVIFQRLLQPLDHKLISQFIIDQKCSQFMYWDSVIDFKRLNLQYSHQYKYYSCQQSNEATNIQKDIDRTFSQHQYFKQIHNRQRLQRILIALSKIYEELGYIQGLNQIAGCFLISGLSEQQAFWIMYYILKKMKYATIFLDQFSQLKFLNFVVAVFLRNYVPNLSAQFLLNKIDIGIITTRWFLVIFGYDLPQQLLLQVWNLFLQKGIKVLIKISISIFRLVSDFENIEDLYEMLKDELFDLLDSNEQYQIKLIEYFKSFKITNRLINELRSKFETNDESLTLSFDQTQKKHYWKKGGDSARSLISSFNEIISEIQEERDTFYQKSQSFLNVCFPRLINVNYLVASEGKKSLSIKIEKRPQLIKRQILSQQFQMTIPQSANGDQESNIYADELQSKMSPNDDESEDIIERNKSQDFKTILNKYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SG34</accession>
    <name>A0A8S1SG34_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0070317</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SG34"/>
    <dbReference type="OrthoDB" id="304746at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8138796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="130" mass="15170" checksum="8A2BC7124BD55330" modified="2022-10-12" version="1">MRTSCLGPKLVLSRAQVEDQEDIPEEPDDFEPIIIQRKKIRYKSCRSDRYDTKGRKIAKGSGYGINFDNFITVCVFEPEEEVVQIKDTLQLCTQGIDKEKINPRLDQRFKEEQTENEVILFSILKQSKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SMS8</accession>
    <name>A0A8S1SMS8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pre-mRNA-splicing factor CWC15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0090180</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CWC15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SMS8"/>
    <dbReference type="OrthoDB" id="30179at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006973">
        <property type="entry name" value="Cwf_Cwc_15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12718">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12718:SF2">
        <property type="entry name" value="SPLICEOSOME-ASSOCIATED PROTEIN CWC15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04889">
        <property type="entry name" value="Cwf_Cwc_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="110"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="123"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8140607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="259" mass="30709" checksum="34EFA7E2C50CABF1" modified="2022-10-12" version="1">MTTAHRPTYRPAIGGSEQGGNKMLVHSRSYHSKDLPAYLILKMRKPGQGTQAELEQKDFKMDLIKREEEGRRQRELKALGVSETSLAIEATQKNQNKDVQEPMILKKIKMEEKEIYPQDADDKDFIKSSDDEDNAQKPEKPIVQQQELKIEESSSSSSDEEDDDELLMREYQKIKEQREIEEKKKFLEKQEYLEKNREDEIIKGNPLLISEDYSLKKKWYEDTIFKNQSRLEVKEKQRFINDAVRSDFHRKFLNRYIQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SNZ5</accession>
    <name>A0A8S1SNZ5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0100156</name>
        <name evidence="5" type="ORF">PPENT_87.1.T0100157</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SNZ5"/>
    <dbReference type="OrthoDB" id="47374at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031415">
        <property type="term" value="C:NatA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000006">
        <property type="entry name" value="Putative n-alpha-acetyltransferase 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051556">
        <property type="entry name" value="N-term/lysine_N-AcTrnsfr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42919">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42919:SF8">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="7"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8141507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8141508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="179" mass="21200" checksum="CE4A1E48349D4D13" modified="2022-10-12" version="1">MEQNPKLAFGEINYKNLEQFKVITQKTLPVTYSENFYIKILTYSDFSTLGYYNDIAVGAITARIEEKDGRKTAYIMTFGVLDAYRRLGFGTQLLNELINRIRSHEEIRTIYLHMWVSNEIGFQFYSRHGFEKTTYKKNYYTDIDPPHCYILTKRLYPDVDPPIPYTEEDTEKEQEIAQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SQN9</accession>
    <name>A0A8S1SQN9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0120026</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="304175at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="611"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1309"/>
            <end position="1399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1309"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1373"/>
            <end position="1399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8143075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1399" mass="167729" checksum="51C57DA79973AD46" modified="2022-10-12" version="1">MIKNYVKRALLQRYLPTTGCFYEFSESQDTEKKKTRLKYTYEEQQEINRQKQSNFLLKRILLSYYRYKPNKEEMIKKINQYFNPKKQKAEEAITETQEGEEGQEGQKPIKRVSRISRTSKQRIFRDLVHEKKIFKYKRDYIFSNKIRPYKPTKEDITEQMNRELTGQKQITNPNLFDKFKQRQRDFENYNLTNLIMRYDSFQPSQVYEFLKSHFTSGLYGFMKEEQFTTLIFNRPSFQIYLNQLPEQFYVHYQKYLNPENINQIIKEIEYKNTKFLPILRFCELKNKELYFEDQEKFDQFLKKVPEQDQLLLQYFFFNENASKLLNYESFLKGILNIQWGGQVNQESLKQQLVTLLNVKFKINLNKELQLYLADLFFGSETFDKAIIAIALQYLLLQRLTHAFPIKDILEIFKQTMGDERYTFYLEQELPQILQNLNVKTYYSLYQKGLIEYQQQKSKKPLTLQNYTNLDGIFLKGKYIEVSQFQIRYNPSSSNLIYSNQTIAYDVKENAIKEAVLQNSTLLLEKELSSLFHYKNYKSDIVDVAHRLVYRKNLQHRNFLRYNLTKQFLQRKRKLRKYKHRAEISKELADLQTRLRKHSKIQPLLQTICPVQVQPEQTPTEEGQEKPQETQETTTEEQPQEKTLEEEIFSLTEEKQQYNQIRLARVKTFTKFSKILHNIIIENRPMIKEYREKQFVQKLKSNFCTQKYQEIPKDEGTGFMNYLKSVESIDLEVKQQEELNQLQQQKKQQQSRKHGKILLEKGETQQLKSIFSEDGDNLIRIRQEQKKQSEMFQKVEQPQRFRRTKASDTNEQVHKLPFESTEQDKPIQPQLQEQYVIDNQLYNTDLSAKAHFQIEQLFKEDQSRQQLVEDVQSFLSGIKSSEDGYHQYLISELIQALEAKKPLESKIIFTQIICQVKNKIEVNEKIRVKYTTQYGDIEKNYGKYINQTCTPFSIGSKGYNYLLDYIMQEGNYNRLLYKRVLAHYCLYETVLYDSTFEKITQITKNNKIPQTCAEFFYAFLSTKITINFTQMRKIVNLLYQFKHFEAEAEQLIIGFYYAYQWVLKFEELQPIFQRLINQNKRDRYMSLYESLKDQLMGRKLRQIPTQDPVEVNKIMTEYKQQQKDQLKEFVRKFSQLLLDSKLPDDARLVINDAKRFRYQYDILDYRLWLLSHVNFFEDALKIWADYGVKFPNVYDITMLKAFLQLLQQQENANESDDTKALFQDVIRVYIFEKKIPLDHDLIFEITNTYKRLKEFTSYQNFMKFLLEIRYKCIPSHKYLFYKMMNFTEIDSVKETIKGIIDILFKDEQQNKRKDGKQKQNKQEEEVNYDLGNKGPNPLHIQDLTKENKLNNKKKRGVLVRGGKSKFSQIETDGLADKNSEEEGKTGKDGKATKDAKATKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1STW7</accession>
    <name>A0A8S1STW7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0100400</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1STW7"/>
    <dbReference type="OrthoDB" id="45882at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8141972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="227" mass="26854" checksum="58B9FED262700011" modified="2022-10-12" version="1">MDADIFREMFPQDYIKRFLEKNVRIDGRQFKQPRQIIIEFGEGFTNYQYADCFISVKHIIREIESKEVDLNQCIRLSIIMDNQEDQSNIDKVRIEKHFLSQIQDVVIQIVDPQRLRTLNDKVKQIELYIRVAGGDGSLLSHLINCVSFSLQQIKHFETQNSIIYKEIKCQTFAVFEDQILVDPSDFEEKNSSIITVIHILEDEQFILKKYDGKPMNYNLIQQIIQLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SU08</accession>
    <name>A0A8S1SU08_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0110381</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SU08"/>
    <dbReference type="OrthoDB" id="308913at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8142876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="160" mass="19019" checksum="65C0BC48EE5ACAEA" modified="2022-10-12" version="1">MGSVCKNMKNTQDLKQIQMNCKNNKTFKKLKRADFQHPKQILDFDSSYSICSERNSSQFSKTIGHINTSSCIYMKRRDNQFFTRRVNSSLNRNSLNLLKKDDYNSQSDLSIELIDDAQLKLIIPYHARRSWSKKRVTTKVDLLKKVKNQKYLERFKRRKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SU47</accession>
    <name>A0A8S1SU47_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0120245</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SU47"/>
    <dbReference type="OrthoDB" id="10253408at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035942946" evidence="3">
        <location>
            <begin position="16"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="5">
        <location>
            <begin position="44"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="4">
        <location>
            <begin position="162"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8143470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="373" mass="42731" checksum="F5744C8C43B0D0B1" modified="2022-10-12" version="1">MKIIQLLGLVSYVLGLTYSSASLSDKLHENGSVYGWREEEQKQFKNWVQENQKEYNNEFEMIYRMEVFVKNYRAMKHHNEQLPKDVWGLNVFSDETSEELMDKLFMKKDFEEHYETFNEDDINAIKRDTLSHNSFLQADKTVVVVKKVVKKASAPAKATPKNPPSLDWLKQVTEVQQQGRCGSCWAFAVQDVVISRLAIANKNKLDQLSKTHLIDCADGNTAGCDGGSVSDAFDFINQYGTVYEKDYREYDQKEDECSKPKGSIGYKQFKSVQGLTKFTNNDIETAMQTGPVTALMYADESWLRYTSGIINTCGYPKVSNYQHVVSFIAYDTQTWFAKNSWGAKWGMNGYFQVQKNGDANCIDKIKKITYPVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SUN3</accession>
    <name>A0A8S1SUN3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trichocyst matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0120249</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SUN3"/>
    <dbReference type="OrthoDB" id="294097at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035713433" description="Trichocyst matrix protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="205"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8143478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="363" mass="42028" checksum="7E940024AE25C7FC" modified="2022-10-12" version="1">MKTLLLLLVVGVMCQNEVDTVEKLLADLKGASAVELTALNTDYTVSKNLKENIIFNLGKAYQEQNFLCASRDKSVSDRESDIDKTNKYIAYLQKRLVDNSNRIHTLDANRCTHSSNYIERVKNDNMTLRLIIFLRNSLQNLDNNQLNRYGAYVEKFLTMYKQAKISELVELAHEFADTMFIDWNQVTPSSQGNINTVKGQLIEMLNDMERYIREQIKNVQNLEIATGVTLADFKGAIDIENEQINYDLSSEQKNLIKLEEQLVQARLAAVNCREKGVALQQQQQRAIEDLKNEEIQYNKNKARLQEELNLFTDVYRIYSTQVGTSEDKFKQRVDDYVNDRKVDSYERSQYSLSDAVRKQVQNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SV86</accession>
    <name>A0A8S1SV86_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0120084</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SV86"/>
    <dbReference type="OrthoDB" id="292259at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="339"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="171"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="340"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8143189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="407" mass="47973" checksum="CEFC765AD4E85F8C" modified="2022-10-12" version="1">MYKSKRTQFGGSTASTLDLLQAQDCLFCQQSQKFCCLITRALYIKYSASQNYYYQKDINIILLQQRSKQYTTYKEWTYYDTNEYLKRFYSLTDTRQKTIVLTEYYKFHRDIPRLFMLPIALTLSKYHDKRRRIEYIRISRMLGGHIEQPTPTQNSIQTSLQNLLGQLNFTKQEQSNTLIEIQKNLKEAINTSKNIQKEFDSRKYNLPLQLKNSDYYNKSKLDHKSDTNSRLDFKENFNDLFQKKKPLKNSGIQTQREVSQPKVYSTEKNCKFQVQQIPLTQRNGNNTKQSISPRPTTQRSIKPQIMQLLKPNHNNNNKLKATNIILECLMRKLNLDQKAITKQKSKSPNNPSNQQVKRKRRVMSTNQETTKIDLSQLKQKQSQLLQSKFDQNIINKGYKTGRASAMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SX12</accession>
    <name>A0A8S1SX12_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0130307</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SX12"/>
    <dbReference type="OrthoDB" id="301727at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8144603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="178" mass="20471" checksum="3E56C3F986D39E30" modified="2022-10-12" version="1">MGGICQMQITISEQENNISKQARIYIKGPPKVQKPCPINNLQIQSDVVPIEKENFISIPKIISQQEEFAITEQEKAIKDVKFQLTEEIIFLQERDQQEQQNNSNVEKNSLKNQKKSSCYNICKGKSILKNKIYDGSNPQSPKTQLKESLTFGSQRSLKKVTFNKKQKVIYSSFRNIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T4T3</accession>
    <name>A0A8S1T4T3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">60S ribosomal protein L7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0160349</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T4T3"/>
    <dbReference type="OrthoDB" id="28644at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01657">
        <property type="entry name" value="Ribosomal_L7_archeal_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.20:FF:000003">
        <property type="entry name" value="60S ribosomal protein L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039699">
        <property type="entry name" value="Ribosomal_uL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005998">
        <property type="entry name" value="Ribosomal_uL30_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035808">
        <property type="entry name" value="Ribosomal_uL30_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_uL30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012988">
        <property type="entry name" value="Ribosomal_uL30_N_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01310">
        <property type="entry name" value="uL30_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08079">
        <property type="entry name" value="Ribosomal_L30_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL30 N-terminal eukaryotes" evidence="8">
        <location>
            <begin position="14"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL30-like ferredoxin-like fold" evidence="7">
        <location>
            <begin position="91"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8147380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="248" mass="28660" checksum="96965D4B0407D954" modified="2022-10-12" version="1">MSQKKPQMQVEQKVPENIVKKTARDTKLREAVAKRRTERLAANKTRRAQWEKNAQAYENEYKAADKSLIDNIRKAKSEGGFYVPAESKLILVVRIRGINTLNPQVRQTLRLLKLRQLHNAAFVRVNKATIEMIRKVEPYVTYGYPSRAVIKNLIYKRGYAKINGQRIPITNNNVIEQQLGKVGIHSIEDLIHEIVTVGPNFKEANRFLWAFKLRGPRGGFIAKRKSFINQGDWGNREDLINDLVKRMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T551</accession>
    <name>A0A8S1T551_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Deoxyribodipyrimidine photo-lyase</fullName>
            <ecNumber evidence="3">4.1.99.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">DNA photolyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T0180094</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA).</text>
            <dbReference type="EC" id="4.1.99.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA photolyase class-2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.3" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T551"/>
    <dbReference type="OrthoDB" id="496749at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003904">
        <property type="term" value="F:deoxyribodipyrimidine photo-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000719">
        <property type="term" value="P:photoreactive repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.579.10:FF:000002">
        <property type="entry name" value="Deoxyribodipyrimidine photolyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032673">
        <property type="entry name" value="DNA_photolyase_2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006050">
        <property type="entry name" value="DNA_photolyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052219">
        <property type="entry name" value="Photolyase_Class-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10211:SF0">
        <property type="entry name" value="DEOXYRIBODIPYRIMIDINE PHOTO-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10211">
        <property type="entry name" value="DEOXYRIBODIPYRIMIDINE PHOTOLYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00875">
        <property type="entry name" value="DNA_photolyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01083">
        <property type="entry name" value="DNA_PHOTOLYASES_2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51645">
        <property type="entry name" value="PHR_CRY_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Photolyase/cryptochrome alpha/beta" evidence="13">
        <location>
            <begin position="18"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8148495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="453" mass="53167" checksum="C0F74B683763DF97" modified="2022-10-12" version="1">MKERIKYLNDKKVNVKGKYVAYWIQASQRTKYNHALELAIQKANQEQLPLFCFFGLTKYPAANQRPYHFMLEGLQQLKTSLANRKILFGVAKQSPDDLAISIAQNAKLLIVDCGYLRIQKQWRKKVADTIDCQFIQVETDVLVPVEQASQKEEWAAKTIRPKIQSLIKYFSQELNEETLLKKMDKLPFEEYDISNIQKVIDDLGVDKSVSIVQQFKGGEIEAQTRLEEFLNKKLKNYSKNRNDPSLNATSNLSPYLHFGMISPLYIYLEAMKFPASESRESFLEELIVRRELSMNFCHYNELYDKYEGLPDWAKNTLKEHAKDKRDYIYTLEQFEKAKTHDVYWNSAQLEAMHKGKISGYMRMYWGKKVIEWTESPQQAFEYLVYLNDKYHLDGRDANGYTGVAWCFGKHDRPWTGRKIFGNIRYMIDSGLKRKFDTGQYVLNVNQLKKQSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T6N9</accession>
    <name>A0A8S1T6N9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0180316</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T6N9"/>
    <dbReference type="OrthoDB" id="298175at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00060">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46210">
        <property type="entry name" value="FHA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46210:SF1">
        <property type="entry name" value="FHA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="165"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="392"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8148935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="498" mass="58909" checksum="B0730B74BDB7854F" modified="2022-10-12" version="1">MNNDNNNDNNDNNQNNNNNNNQNNNLLSPFNNPLLDLQQFLDIRSSQQFNQFLNFYSNGEQQQSFFRMNAFTSRSFNQDEEIMLYLQNQSRREPSSPRYKNSNLDKYMQKRAIPIHNEELNKNEPYIKVTVMRNTNYPNTNHSNEYFINQHGLIGSHKNADSEDILIGRSHRTEVIPNDIMLPEDRIISRIHCKLVCKHYFRQDQKIKLIYKLAIQNIRTSFNFLLPQRAIKLISQFLDCPRYAYVQDLGSLCGTHLKIQKNEPNIMKYDQKYSIGSDTNFTVLLCESYKEQGFEIDDQFFRLLRKITILKQFKHEIHFSDQQLFQRFQNIQLQEDDNEETKNYAEYYKEIYNKLKQYKAPIVSIRFGGSGLEAGKIFNIFIHSKNQDSIECQIGRGQENHVKINSNTISRKQSRFKYSKNLKSWVIFDGSKERDSANGTWVSLSTLEQSQKKQESDLIILKNNAEIKISEFILKIEFFEGKKQNKINKQILNELRNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T7F5</accession>
    <name>A0A8S1T7F5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">uracil phosphoribosyltransferase</fullName>
            <ecNumber evidence="4">2.4.2.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0190331</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uracil: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPRTase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.9" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T7F5"/>
    <dbReference type="OrthoDB" id="106623at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004845">
        <property type="term" value="F:uracil phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008655">
        <property type="term" value="P:pyrimidine-containing compound salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000023">
        <property type="entry name" value="Probable uracil phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001097">
        <property type="entry name" value="PRK00129.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14681">
        <property type="entry name" value="UPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035781943" description="uracil phosphoribosyltransferase" evidence="10">
        <location>
            <begin position="18"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="11">
        <location>
            <begin position="58"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8149831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="274" mass="31306" checksum="E073E491A01F87ED" modified="2022-10-12" version="1">MFKNLSKFVCCLGLVNGTQMACSQLQNDHSYNRMQHYNFLSQDQLHNLLNKHKNLFILRENQTQHILSMIRDVNSDIVTFRQNSDRLIRILLEQALAHIEKKNSIKQSPLGFYDANQVKFSDQEICIVSILRSGNAFLNEALKVIQGASIGQILIQRNEETSMPKYFFEKLPENINEQQIILVDPMLGTGGSASMALKILQNYGVKQENIIFLNLVSCEQGLNKVFKEYPKIKIITAQVDPILIKEINYLAPGIGDFGDRYFGTVKKHTRAEQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T9C1</accession>
    <name>A0A8S1T9C1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0180368</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8149020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T9C1"/>
    <dbReference type="OrthoDB" id="304074at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035779416" evidence="1">
        <location>
            <begin position="18"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8149020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="214" mass="24429" checksum="305326500DE765ED" modified="2022-10-12" version="1">MKLLLILLLCLSTYAFTDQMMSLEELAKFDIKSVDCTKIDQISLIEKQMQQWEDLLKHQKAISHDIKILKSMEHLLSSKSNSFLEIQTQISGKKLMKKLHKLELPLTKSKIGLAQVQLLKEQCKGLDSENPEERAQAKKELCKLLKEYVNNLNNCKQQCTNSPVTVIKIKGQIKDLEIIRDNCQNGKISRVKVVTIDGENQEYKVAADGTTEIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TBF1</accession>
    <name>A0A8S1TBF1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0200327</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TBF1"/>
    <dbReference type="OrthoDB" id="309603at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8150555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="154" mass="18453" checksum="D102CC02F03B8E13" modified="2022-10-12" version="1">MNQDLDQKSIDQIIDKKLAMMEAEYEMFKKYDNPNYKGADYPIDQINNLNIENEQKEKDEEKEKEKEKEKEENQNLDEDDEFQEFQGYEKMDENSDKEEDDEINYDEVEHIDPFAKPLDVDLIKKCMAEIQLPTPAWAQSMQGWEQKLSQMKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TD74</accession>
    <name>A0A8S1TD74_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0200236</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TD74"/>
    <dbReference type="OrthoDB" id="304296at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="80"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8150390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="202" mass="24112" checksum="C83B92689E411E3F" modified="2022-10-12" version="1">MYDFSRMQKLVDRMQKLQPSKPPPDTRFLPEELKKEKVIEDPKKLEKKAKRQLLIKNQKGSSGLKPVEDERLLLIQKENYLKKKQRELEMEQVKKELEEQKKLEKQKLEIETQRALQAVKGKKELSQLEREEKIKKQNDNEFLKRKQKVNNSIQMSSIDASYVSQNQNLNNSLIKKQILDYHTLPKEQYNRIYRQPAVSPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TDV4</accession>
    <name>A0A8S1TDV4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0180395</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8149062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00106">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000112">
        <property type="entry name" value="Kinesin-8, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF29">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0505">Motor protein</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="3">
        <location>
            <begin position="4"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="367"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="91"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8149062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="510" mass="57711" checksum="6CA4E52A1107AF1D" modified="2022-10-12" version="1">MQEPFKVSIRVKPYEGRSRFLTQRIGKETIIQVECPTQTIRDPDTYEQRSFAFDNVFNEGESTQDIYDTSISQMIYDCVSQGYNGTILAYGQTGSGKSYTMFGNLYDPLVENDGLVSMVLEQLFQMNVKISISYLEIYNEQIRDLIGDQVGLQLNEDPIKGVMLQDVQELQIMTIDQAKSIIINGNQKRVMAATNANQFSSRSHAIIQLFVVNQQYQCKLSLVDLAGSEKANVNEGSKGIRQMEGANINKSLLALGNCINMLACDQSMKKFVPYRDSKLTRLLKDSLGGNTKTLMIGCVQQVVQCHEESINTLKYASRARAIKKKIVQNIKINDITNCNCNCNSENVGLLQAEIEALKQEVSYQCQLTEEEIEIKLNLKQIDQLQLQNREGLEYLLQQMNSEMIDEDRLKNEILQYERAINENXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TE70</accession>
    <name>A0A8S1TE70_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Metalloenzyme domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0200125</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TE70"/>
    <dbReference type="OrthoDB" id="272139at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051377">
        <property type="term" value="F:mannose-ethanolamine phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16019">
        <property type="entry name" value="GPI_EPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000112">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006124">
        <property type="entry name" value="Metalloenzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039524">
        <property type="entry name" value="PIGO/GPI13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23071:SF1">
        <property type="entry name" value="GPI ETHANOLAMINE PHOSPHATE TRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23071">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01676">
        <property type="entry name" value="Metalloenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="398"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="442"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="468"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="517"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="557"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="589"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="626"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="680"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="718"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="741"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="782"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="830"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="862"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="domain" description="Metalloenzyme" evidence="2">
        <location>
            <begin position="173"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8150168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="881" mass="103666" checksum="C73F4FEA4D6D2B9C" modified="2022-10-12" version="1">MKQTKCLIITLIVFKLLGLLIFFRGYFIDRRYLLDTTEGTMKPTQPVILLVIDSFRIDLAVNQHFTFIKNMTKNKPDQSLFFLSFAEVPTVTGPRLQAMTSGNFPPLSKLLDNFHASEIKEDNIMFQMKKFNKKTLFSGDDTWIGLYPDQFTVQFPQKSFNIGDMHSVDEFNCDKIIQNLDQGYDLIVSHFLGLDHAGHKNNRVLNNSDLNQKLNQLDQIVEFIYERMSNDTILIVAGDHGMANDGNHGGNSTEETNTLFFATRKQGKFYPNYMEKIPELKDNYQSTLINQSEYIRKISQIDIVPTLATLLGIPIPFSNLGYLMNEFFNSEEHCLNNLKQVWNFVETVHSRQGKFSYYQKIQWQNQYSEVKTCKDALILMNDIQMVARKIWNEYDIPLLNLSFVFHFLVIIFYIFVMVILFQAQENDDPITIQQITAAIKHIFYNNKQVTIIFAILSALLYIFYEVEILITIILILLMIYINFAIFIKIKKNHIQNLNQYQWDLIEKQNSIKLNNRFSKLIQISLFLFFIYKIILQGELLLSIQQKKFEERYLYNEIIELLILSSVICIFNFISRLIDTQEEISSIIKIKDSFISFISAITIYYALNFEQYYLKFQNYQFLNESHYMLYMPVIFLHTCLFMYQKKSNILDKIIAQSGLILIDIFYIYPDTTIQDQPYQEFVILHIPKIIYAISIYYYFSKDPIFLLLSCIIVSDKYGLAIYTYEILIFICLYYHWRNLMKFSMISFLAMISLICQITWFIFGNRCTISSIKVDRAFVGVKEFHLWLNPLILTLFLLGPYLAGLVFIKYIGPKLCKYGELNERQVKNSNNLFKLMFLFNFYIQIQFTQIHSYENAGGLIDVQFKYIFDAVTFLIVSLLMLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TEF2</accession>
    <name>A0A8S1TEF2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0190178</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TEF2"/>
    <dbReference type="OrthoDB" id="297867at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8149576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="565" mass="67224" checksum="4E6D8BB45F19EE86" modified="2022-10-12" version="1">MLNKYTLEFLFKDQAEQYENYSKQSKLREVTYFTIYSVSFSILNTTKFILRDSIPYSILSGISVFAILILWKILQKKLHLINLIVTIYQMFLLCAFKMPQLFDGTSNPTLDQSWYYGFQCGYLHFSVYLLSANFIYQTFLLIIVIALHLYQDNIDIKDGIINIFLFVFLAMSMIAIKYNYEKTKKEQYVQSCQQERWEQLLTKVLSSSILLVSHDKKQDQLQLEKENKFSQILFRLKDSENLRLLLRQLILSSSRDPEDSVSNVVHINMENTLRRFLIHTQEFADSFKTYKYTVQHQKTQKFYNVKILRCVFNNKLQCLLIIDPKKKYFYHQTQVLWRILQNLTETANEQSRFLIKMLNKFNQISIKQKFPDQKFYFNHNFIQNMQIIFQNKSNRLKLQDITQNSLQNALDPLIGSDSQKVLLVKSFIHDISIIQQLLISCFYIIQQIFKDLKILQILIENQKTEILFQIKIKERDKTIQTKILEILQTNWVKSPKGFFHKTNVPQSLLERMSYLTQQFQIPINLLLNISITQLILSEFGVYNHLDIIQDNNEFTFTFSLVPQIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TL19</accession>
    <name>A0A8S1TL19_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamine amidotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0220138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8151856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TL19"/>
    <dbReference type="OrthoDB" id="287637at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000109">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044992">
        <property type="entry name" value="ChyE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YLR126C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695:SF5">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YLR126C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="1">
        <location>
            <begin position="185"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8151856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="408" mass="48181" checksum="B8065D002F115094" modified="2022-10-12" version="1">MQIKVSLIFHKGQQLLINSQQNQNCTVLESDGNSCAIAAAKLIIKQFGSIDITKTSLNKHHNFEKIINWEAYQQTKDSYNYIRELEIYRNPHFYLWTRFFTTAPIWEIEDHTYYIIELIDQDLIYFNFNNRRELNEKHTIEQVILEGQHLKYNRTIIFLCVKDFDQGRYHAPKMYIDTIGLFNGIYRRKQERWLCYNVTQGSYPDEEILKQTNVIIIPGSTSSVYDEKAWIRSLQNFILNVYENYSDIKFMGICFGFQLLAQTFGGIVVPCKNRIKHKGFYYYGNEKLTIKNDFFQIECFKELIQNDKLIINKAHGDIVSKLPEKFINYGSSKTAENEIFISEDLRIFGMQSHPEYSSQQILLFISGIQFAEGNKCFEDYYNDPNNKNFVKEKGDLLGLLMCHKFLQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TLG9</accession>
    <name>A0A8S1TLG9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase C1A papain C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0230127</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TLG9"/>
    <dbReference type="OrthoDB" id="311597at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000196">
        <property type="entry name" value="Clan CA, family C1, cathepsin B-like cysteine peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0865">Zymogen</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="4">
        <location>
            <begin position="131"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8152587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="358" mass="39785" checksum="39A5E5BE57265F65" modified="2022-10-12" version="1">MSQVSQRQRSPSPQSPEKIAQREKAAQAMKRRNQTQPSFFEKYAYQLVIGAFGFVCVYALFSILTRSSKKLTTAPVIDEEEIAAHNSLGSYLQGPNDFFKDWKFSDAKFIFNNHLTFKGKIQQCPESGVIIPESYNFRDAQPECAQPIYFQGNCSSSYSIAAVSATSDRLCKSKNGEFQDQLSPQSPISCDDKNYKCGGGSVTRVLEVGKKQGFVSTSCLPYSGTEDAKNNCDALFSNCEKYKIHDYCVVSSEENIKREILNNGPVVAVIQVFKDFLVYKGGIYEVVEGSSKFQFGHAVKVIGWGKQDGVNYWIIENSWGDTWGLKGLAYIAVGQNQLQLEAYSVAPIIAVPTEKIGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TM32</accession>
    <name>A0A8S1TM32_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0220273</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="5">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the peptidase T1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8152089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TM32"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03749">
        <property type="entry name" value="proteasome_alpha_type_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000016">
        <property type="entry name" value="Proteasome subunit alpha type-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035144">
        <property type="entry name" value="Proteasome_alpha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 4 5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="6">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8152089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="248" mass="28179" checksum="0E192D3207C395E3" modified="2022-10-12" version="1">MLKHLYDTDCVTWSPQGKLFQVEYAMEAVKQGSICLGLKSNENVVLCSLKRQPSELAGYQEKQFKIDDHMAIAIAGLTADARVLCKYMRTECLEYKYTYESHHPVGRLVYKVAEKSQHKTQSGAKRPYGVGLLVAGIDPNGVHLFETCPSGNYYEYKCQAIGSRSQAARTYFESWFHLFEKSTLEQLILHGLSAIKKAIMEDEELNEKNVEVGILGKNQKFTHLNAQELRDYIAKLDAFNPNSQIQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TME4</accession>
    <name>A0A8S1TME4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0240052</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TME4"/>
    <dbReference type="OrthoDB" id="159299at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045238">
        <property type="entry name" value="Tim23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15371:SF0">
        <property type="entry name" value="SD19278P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15371">
        <property type="entry name" value="TIM23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8153143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="159" mass="17123" checksum="1E876A92EFDDBADA" modified="2022-10-12" version="1">MGSKAENQIEQYSGQDFGDKISFTVGVSYMLASSIGLLKGAIEGFPRQFNMPKKLILNNFFNAVGKRTSTYGQAAASASMLYYFVGAGMNLLFEDELADINQLKKNMLCGAISGAIYKSTLGIVPFFVGGIVGGSLIGGVTLLVENFNRRGIVAFEMKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TQ39</accession>
    <name>A0A8S1TQ39_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0240010</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TQ39"/>
    <dbReference type="OrthoDB" id="426293at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="305"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="349"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="381"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="7">
        <location>
            <begin position="487"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="712"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="737"/>
            <end position="752"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8153059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="934" mass="109729" checksum="BA84B400DACC6D62" modified="2022-10-12" version="1">MSAYEDPISSPNMASSIVFMSTNYIIFEDDEFLNNFKMKQSQFTMLDKVKLSREVSEQSILSQDSYDFEAKKGFKTKIWKDRALNIIVLVARFVTYLLTNSDKFKLRYLDHRQFKVIGDKASDFNFYLTHKLIRGKSKKGSLELLSTKIKLYLSFLRVFSSIIKPIKPDNLLKLYWDVIVFLILLINILYIPLKISFQISVVDGADFFLDTLPQYVFVFEILLNFNVAYYSRGVLVLNRAQIFKHYLKGKFALDFIVLIPFMIGRSNVPYIEFVLLFRVSRVMYIFENIVETMNLRVNFASVIDIISLMSTFLFASHIIACIWHFIAIQESSFEENTWIQRANLVEHNWQARYITSFYWACITTLTIGYGDIIPVTQYEKIFVIFVTLLSSIIFGYTISSIGSIFAQMSENKNYLRDRMTLIDSFLKKRGLNKDLQVKVKKFFEYFLKQERDQESECEKLMIHLSGTLNREVKIDFYKNMLCTSKLIRQNFSAQFIEKLCILVKEQSFVPEEIISIEGQKVDKIYFILKGEVEAYISNNKTIKIYSRSQAIDEKSFISQHPALFSTRAIKFSKLAYITYDDLLELLKYNQEDRENFYYVKHQIEFGGRVKMGGCELCHQNHDFIKCPFVFYTPNSLRLFKKNDHDEKQHRRFKLRSRISQKLHNNSLGQLKNQQQIVFNYCACQGLLNEIGPNSEFILSNRFNFQQDSAQEDIEPSLTNSDNESEYKPIKSQNYLQIKRDSKRKVSENKDNQSIKSKKKYTRISIQKICQQNRNSGQYERENQILERRHKSSTKRATLIQNLMKLEQSERLDDDPLQTQFQQAIALTLQTQQQQGLSQLPSPQLQQHSGQQLKFFDVDQVQYIEMDIAKLMLTKECDIDSQKEMEFYDTGFNFEIVVGKLKKASKKLPLKLKRKKLIKSLARSQSIRHISQTRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TRN8</accession>
    <name>A0A8S1TRN8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0240315</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TRN8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8153619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="180" mass="22242" checksum="A1F00D8E024FBA9C" modified="2022-10-12" version="1">MFQKILNQKVKHQEDMNRSVVKEYEVVDKKIKTLHEVFNWKAHLKMNQFVKQSLRKIRMKLLHSSILIILRINQNKYNNLQQKNWRINNSPTLYQYQCQHSMNHIIILILHRQIQKFLEVNHLEVQMKQTNLIKRRNKMRQEKLEQQKIIQDIRYKNRKSQIVRIRSYQFTQNETCILYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TRQ6</accession>
    <name>A0A8S1TRQ6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0240327</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TRQ6"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8153639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="76" mass="9265" checksum="6B3BFC0597DD05AB" modified="2022-10-12" version="1">MLLLLWYKRTMRVINRKQEMLGKFYYKQKRLPKQIMYRCAYNNSENFISRCLYNGVRQAPKSANCTVYKEKQIICS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TT63</accession>
    <name>A0A8S1TT63_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0260374</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8155223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TT63"/>
    <dbReference type="OrthoDB" id="295547at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47823:SF9">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_10, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47823">
        <property type="entry name" value="ION_TRANS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="295"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="334"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="358"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="460"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8155223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="823" mass="98041" checksum="9596856FAF26B7B9" modified="2022-10-12" version="1">MSIKQVHPTNEVEEQNEFKKGLHSVQTFNDLRRRRIQVREATLILSPKLQQYQHMLQPKISQFTSQDQIMEEEDVLVLDGFKIKFNRAIRRLLIILNFARAITYFSKQKNKKNITQSSISRQSIPFLPHLKFIRLWREIISFNTVVANIVYPIYITYSEFDSYDFITIVTFIIDFLFFVDIILEQMICQIDNNNILLRKFSSIFIHNLTGWLIFDILTIIPYKLFVEKMEDTFQLRDYFNLLKLIRLLKYFIKTDRKILYHSADSKKSEISIEDKLSFFDYFSLIDAKLLSVLNIFKNMLILISAFGCMWHYVKYYEGISMGEDYSNWTSYIDGLFWAIQTVTVVGYGNVSISTSMQYNLVIVWLLVGVGFYSFTIGNLAQILEQQTSAEIQKEHLEDLENLMDSIVIPDWLSDQVSNFLTYNLENNSFWNYRKIIDSLPGQLKKYTICFSQQKLIVNTNLFKLDINIASKLLPYMTIFYYQKYETIYHVGSPSMDIYFLITGEVRLCDEQGRSLLNIIEGSIFGEMEILDQVNRKQSAIASKNSIVIIFPVQIFLDIIQTDESLSFEIEQLGLRRKILIKESHLRMRQNQKVRRVSCINYKEQRKPTDISKNIMEKEFKQRVSKESFQFIQTRILKYIFGRNQERKWRLFQKFRDVVSKVMKFNKQKRELNKRRRESFIGLKMINNFKGTTENEQLIIKQIFKLQKQKRQLMKTLKQIINHVKSGLQAPMLITPSPYFYCLLKEQKQQILEKKEQETQNKFQKQQKIKKINPYYQLRHLNDFWSKLQNELILYKNAKTELRYQQMEVDGVYFEIQGLIKSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TV60</accession>
    <name>A0A8S1TV60_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0270094</name>
        <name evidence="3" type="ORF">PPENT_87.1.T0270095</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TV60"/>
    <dbReference type="OrthoDB" id="321763at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="85"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="143"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8155442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8155444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="245" mass="29883" checksum="D22EB607C906D3E9" modified="2022-10-12" version="1">MQQKLEELEKLNKQQRAQLEQNNQEITKLKKDLVDLQSSYEQQLSKERINSQQELKNRITQMQNENTQRFKDQKQQHDVEMEQFKRSFERNIEKLMKEKSALQEQLTNAEIEKIYLKQSTQSLSECKDCIKDLQNPNVVKETLEKLQKRLKECQEQIKQLQIQLKDISELEYKAKEMSQKYQQQLYKMKENFDKQEKELQNQQASFNEITKKFEQEQQKNLQLKDEFEKEKMRLQEQIKSLQRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U0L3</accession>
    <name>A0A8S1U0L3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Deacetylase sirtuin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0310138</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="424302at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017136">
        <property type="term" value="F:histone deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00296">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF10">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8158058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="268" mass="31595" checksum="5BBACBF49423CD16" modified="2022-10-12" version="1">MQKYIKECQAFIITAGAGMGVDSGLPDFRGNKGFWKIYRPFENKYGFTDCANPQFMNYNPNLFWGFYGHRLNMYRNAIPHQGFQILKKIFLNRDYFIVTSNVDGQFQKAGFDINHIYEMHGSIHKFQCTPCDKLYDANQFQDLSIDLEKFIAADPLPQCECKHLLRPNILMFGDWDWISTIYDQQEKRFFEFVEKYKNNKVCVIEIGAGTSIPSIRNLGDRILDNXIYYSFHVFIGLQQQIKQSSVEFDQKIILIYFLYQKFIAPQFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U1S5</accession>
    <name>A0A8S1U1S5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">cGMP-dependent protein kinase</fullName>
            <ecNumber evidence="6">2.7.11.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">PPENT_87.1.T0330285</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.12" evidence="6"/>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U1S5"/>
    <dbReference type="OrthoDB" id="290584at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030553">
        <property type="term" value="F:cGMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004692">
        <property type="term" value="F:cGMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05572">
        <property type="entry name" value="STKc_cGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000068">
        <property type="entry name" value="cGMP-dependent protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000123">
        <property type="entry name" value="cGMP-dependent protein kinase 14-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000089">
        <property type="entry name" value="cGMP-dependent protein kinase 5-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035014">
        <property type="entry name" value="STKc_cGK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF37">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT PRKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0140">cGMP</keyword>
    <keyword evidence="15" id="KW-0142">cGMP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="22">
        <location>
            <begin position="72"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="22">
        <location>
            <begin position="190"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="22">
        <location>
            <begin position="310"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="448"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="23">
        <location>
            <begin position="708"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="13"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00024113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="CAD8159711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="772" mass="88925" checksum="5CFCCD019472B22C" modified="2022-10-12" version="1">MDPRSQNNSENQIHGDDNEDQRSGDEADKLNNFKHNINEGGEVIHENVKKIDMKKTLNDTTFILTCLKNHFVFYNLSEAELENIVNKMFYCEAAAQTYIFKQQDHATCFFILQRGSLEVIVNEKAKRELKTGDGFGELALLYNAPRSASVKCFENCNLWGIDRNTFRRAVEEMITKEYEENRKFMEVVRFFHNLTNEQKDAIAAVLIVQKFYKNQIIVNEGDPGSSFYIIKEGTVSVLKGNKEVRKLYKGDSFGEQALYYNTVRQMTVRAEDDVKCLALGRDSLTKILGDQVHVVTFRNLQKWAFEKNALLSKLTKAQIDKVLDVMKISSCKAGDVILKKGTQANQKIIVIIEGSLKKSKSGITVATKAQAWGEEYFLQTNKAKILDDDIVMETDGVIAEITADNFIDCIGGELEEVIKKNEKILEKKLQKSDQTKKKEAQNIKKSELIHIKTIAYGQFGPVYLVKAKYNQQLYVLKAFNKNQINEQTLEKYLQQEKQVLEIVNFPFIISFMKTFKDTLDVYFLLEYVRGMELFDVIRDIGLLSTYDSQFYVASMILITEYLHHQNIIYRDIKPENFMVDEKGFLKLIDLGTAKIIKGKQGIIRTYTIIGTPHYMAPEIICGKGYNCLVDLWSIGICLYEFMCGMVPFGEEAEDPYEIYEEIIKKDITYPNYLKDKKAKKLMDQLLSRVPEVRLGGSYASLKGNPWFENFDWEKLLEKEIKTPYLPPADKLLPEIEIKSLEQNGRMIEDEIKQSVRQMDGNNQGDQGWEKDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U237</accession>
    <name>A0A8S1U237_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0320001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8158514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U237"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8158514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="220" mass="26562" checksum="7BFD888CCA881534" modified="2022-10-12" version="1">MRFHQNQQMVIHPENLQQINLQLKILELLFMLPQKYLNHQYDQKCDFYSLRIILHALLFGNPSFAGRTFHQELERIKKVSLFLRVEIRRISIKNLNHLYQNSQQLILKGDYRQKKHWMIVGQKNTILQHNQMQGCQKILYKFKQIIIHKPQKKLRQALMSQSSQYKKKFKIFKMNSQSQIQIMMEYLIRMGYQGLFQLKLDTKVIKGIVKIIDLIDVYLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U6A3</accession>
    <name>A0A8S1U6A3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0340194</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8160154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U6A3"/>
    <dbReference type="OrthoDB" id="288385at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051581">
        <property type="entry name" value="Ca-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34524">
        <property type="entry name" value="CALCYPHOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34524:SF6">
        <property type="entry name" value="CALCYPHOSINE LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="181"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8160154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="335" mass="39737" checksum="9DB1A2869A0D558C" modified="2022-10-12" version="1">MLTELIIEICECERRTETVRKLLSQLSQFEPYAAFKRMDKSRCGEISYQDIIDFMLDNNCSLTSLEASYLLQYLDFNRDGRVTYTDFAKQILPKTDLILKETAAARDSYFVLANKTLPNEIEWGLYKILHQELSNFKHLKIIKDLCKESVQQAFQMIDHYQLGYFTAQHLENYFKTNYIQYDKQDICAFFHASDRDEDNKISIVEFNYVIKPFHPSQILKDRERAFYSTPKKYRRIDNSPKPQNSFTNVRSKQFIYKTPVKADLSQPDLEIQKQQLIQQSDFNLIDLFYQQPKATPEMKFRKFCKTILPKNAPPNYQTKPISAKTYQMMLNILRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U726</accession>
    <name>A0A8S1U726_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0340036</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8159872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U726"/>
    <dbReference type="OrthoDB" id="307102at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="241"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="116"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="241"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="274"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8159872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="421" mass="49379" checksum="9FC1DE9394D8FCB5" modified="2022-10-12" version="1">MNFQIEQNLSDNEIQCIYGQLQAKLKQILECLKHNKSIMKEFGQVTSEYGGKIEAISKKSIVDHMKINMNKLFKDMGSQIKLLGSKIIESSQVLKELIIPINDESKQLKQSFNDYVKQIEGRKRDQDEQNKRIEELKSKISSLQKSTYQNEKQITYQLKQLKQQEKMMEEEQKMKYQQVLICKRDLGNFILEEGQMIDYKINHSQSKCYEIIYFSLEQFYTKFNQSGQLKPLLEVSLMSQNRSQQRSATENRSHNNTQFTYQNQKSISPSNHIQKQEQQKSNRNKNEIVFPKNIQNQIQKINYDDQQNQVIMKQVKNIHNSKQESAINCKQKSKSFLQFTDQLIESQDCYDNLKDKSTQQIKISNFNKENQNIAFLSSLMNRKQSGASLKRSENSNASIFNCKGLGYLSNQESLNEPFKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UBE9</accession>
    <name>A0A8S1UBE9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0360191</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UBE9"/>
    <dbReference type="OrthoDB" id="293136at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8161447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="413" mass="49342" checksum="7D5AEBF3D173FA50" modified="2022-10-12" version="1">MILQKREKFRCSIRREALENHFKLAREKAHQNNTNLQLIQKYEQLIQANVIDSDFLQESILLLESTNDEDLIVIILRYIEKFDLSNQSYQLSEALIKQLNNTKINSLQFYLIVSITNILSLQNSETFTQFVSFKLLMSVSEILDSFTELDIIPNLLECISCWPYQFIKMSSDQQQLKLLQSIKTFKQYDELNFYKFLIKITEEIQNDEYLFTYLIKNKYIQEAIDDFKDNKMYVLDFFINLSFLEGEQLMQIISYQFLSPIIFELNKDNKIQGGLYILIRNLLLSNYQIRLYFYDFRIYQHLSYGIQYCLDRLDITNLEEILMCIKSVIQQDEVSYASTKQQIISSDIELMLQNIFLSLENQKDIFILTGEILFIYYTEFKFNGNQKFKEKLKTLDLELDIFDSIQSYIEQFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UBH4</accession>
    <name>A0A8S1UBH4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0350299</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8161042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UBH4"/>
    <dbReference type="OrthoDB" id="307118at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8161042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="183" mass="21794" checksum="56B178CC1223EC5D" modified="2022-10-12" version="1">MNTYYAYESKFQKFYLIRIIQLFEDKTLKLAFKEIKLYSNNQTPRKKMQIQEQSQQIYDGQMYISTKEYTEILQSLPKLQTQVNLSEHLKKQIGLNKKGSFTSVKETVSLIKQKQEKELEDLHAQKVINNFLKMRSMNSIGQNQINNQSLETQQKNKHQVNIKLILVLIIILFIYLLDDCYFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UC22</accession>
    <name>A0A8S1UC22_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0380246</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UC22"/>
    <dbReference type="OrthoDB" id="293256at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8162801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="232" mass="26043" checksum="1FD19231D072F37C" modified="2022-10-12" version="1">MNIRVSVKYCGRSLTAQINPKTNLKEIIQHTIDGGYAKQQMLNNPGVYLSDRSILCKGDELNKPLEVYFPMISLQGPPVELLILREEEFIEYDTAIKSQPKLVDSEPPDIGESQPIKKQSDVSNLSPDSNLLISSQGVVNQGQFTLRFKTTANLSKQGFDFKNLSENNTINDIKDQLFKYFGFHDRSIVDLDLYYNEIPLQGVKSNRTLAQLSIPSGETIEIKARWTGGSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UCM1</accession>
    <name>A0A8S1UCM1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0360325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UCM1"/>
    <dbReference type="OrthoDB" id="538223at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="219"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="261"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="303"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="345"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8161713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="499" mass="56078" checksum="2CE6FC1F353DA30F" modified="2022-10-12" version="1">MQGSFQVKETLADIFDQVKDVDIRNFGAILEILRKEKAQDGIGFLSDIWNQRKLESQILQQVGNLAQADTEQQLSVVRNEIKQITEVLRKLKDHDFNKIDYSFEENEESTQSLINSIKDYRQIFEFLQFLVHLTSIDETLIQVGSNSLHLLVQMKVDLSMKNLENIKIQNTSLVGANFVRCNFSGSQFHNVNISGIKINGALLLNCQWKNLKIKELNIFHGHTSYVNSVCFSPDGNTLASGSVDQSIRLWDVQTGQQKAKLDGHNQSVNSVCFSPDGNELASCSNDYSIILWDAKTRKKRVKIFGHTSFVQSVCFSTDGNTLASGSRDKSIRLWDVKTGLQKAKLDGHSNCINSVCFSPDGNTLASGSNDKSIRLWDVKTGLQKAKLDGHSEWVIVNGSHQSVSLLMSIRLWDVQNGKEIQSADKKFKDILAKFKAPIFSNNPLPESNNITILRISQTPLFQAQGALILKGEFINYEGYDLRSLFKSLGSCILEDFKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UDG8</accession>
    <name>A0A8S1UDG8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0370017</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8161779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UDG8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8161779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="187" mass="22457" checksum="410F4CF69A67DEE1" modified="2022-10-12" version="1">MSYLNQIAIGKQFVYFIQWQMLYFYRNQIQYISQNLYCVCGIEIDYIENLNNCESKYLRYILCYIFNSLTNKCVAFNSQYELHACKVRAKSCYWKGNIVKLQLVVSSISLYRVQTIYFCYEIIILQLYSVYLKVNAILIQIKQQDGLHNPKIRRNLIFLFFQDDSYIQFSNILMLLICLLKKSFDMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UDM5</accession>
    <name>A0A8S1UDM5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0380039</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UDM5"/>
    <dbReference type="OrthoDB" id="297894at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8162444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="130" mass="14745" checksum="D8F8990ED7FCA645" modified="2022-10-12" version="1">MCLYICCWMSQYCYQVQQINENSENFKFDDYSTYLALTIGGASLFILSFLLGLFRTCKEHTGVFWGQLCCLVFGQTLITVGMGFGQTSDDVYGKIDFTFNYPVAEFCAICGIFLQALQNCYEHSLDANSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UEG4</accession>
    <name>A0A8S1UEG4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0380076</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UEG4"/>
    <dbReference type="OrthoDB" id="303489at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="47"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="145"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8162517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="212" mass="25332" checksum="BBE06ECDE9E12338" modified="2022-10-12" version="1">MFNKAVSDKKCTPSPLRKCSQYHVPNSYFSKQPLERSFEPIEKQKTLNDETHEISRLQQIIEQLKNENTRLHSALQQQYLQFQYETQQLNQQIVNMQSEQQQLKQTLIDPSLQNNKMIQYIEELSTQHLNLQILYQDAYLQLQQSEIYSNQIMLLQKELKGKEDEIEILKNELLITTKTNIEQSENQIIYDFLKNIKQQYNDDECNSVHLYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UFN5</accession>
    <name>A0A8S1UFN5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0390292</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UFN5"/>
    <dbReference type="OrthoDB" id="309942at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF8">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="85"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8163580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="555" mass="64757" checksum="230E5D054D2EAF9E" modified="2022-10-12" version="1">MSSQILCNSGVPKLICQKAICEKQNLKNKEIFSCNLQKTITDDAITDCKGCIGKQKDKTINYCQINFQDFFLQLITQRKEEQEILSQVREIERRLQQSVENAKQEIERAQVNIKKIGETIKSMCLFQNNSKLEECLRLKEQTSLDLVKKMIKEFREKITIIEDNQQQNNQQININPDKLNIIYKNGTIIIDDFLKNKLSQMSQMLNSFELQVQQINRTNDNFIQEQNQIINTKYKQIGKADNLTFKKLYNLRFNRSNNLFAFGTNNLDNNKAYCAEIWKIDNNQIQQIQKLQQYPDVTALCFSKKDDSLFTGSLDSKIIYWIPQSNGEYKEQELKNRKDIKGQINYIEINQKSNILAVGSEELNIYTIKNNELLQITHYLQEVKNLAFSNCNQIIIAQFMINNKEKIELFQIKQTGENFEIINLGEVIQTQEIQPITTTRFLHINLQNQINLFKLDNNRFQKIENYSIKKLNSIEKCGIGFCSNNNNKCNLLAFSQNINNNQITQILKINEQNQVTLLQSIQQMGLKLFFSNNGQVLVIWNGDSFVIYKNTSQTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UJB8</accession>
    <name>A0A8S1UJB8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0400096</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8163852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UJB8"/>
    <dbReference type="OrthoDB" id="448960at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15398">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15398:SF4">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN 8 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="156"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="190"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="4">
        <location>
            <begin position="351"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="253"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8163852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="447" mass="52253" checksum="A898739BB5A7673E" modified="2022-10-12" version="1">MMMKQFMLSIMVQNIPTYRFPTQIQPPIPQQVDQNQNQQKFTQEQQQQIYEDFKTILKGQEYDKALNFLQQYKDMDLVNVVDQQSKQICSYIVVQFDNEGLALKFLSLLTDFRVNLNFKDSLKQSILFYICRDGKLKLLDFVLSQNAVNINDQDQYGQTPLFYAARDNKIDIVTRLVQVGANVNLVDTLSNQTALFYSAREGNAEICKILIDNGCNPNHQDQHKKTAQFFAKRFQRKEVMELFNSYFGKSKDDYKQNSNYNNENSKGEQPKQQKKKNKDLPKQAYKLMFTDEYGNLQEVTSSEFAKFQQQYPQIANLIINADELIDENVLNSMKDDELWEKVAKKVLQILWKTKGAQLFHNPVDEKKYGINDYYDIVKKPMDFGTVKQKLNANQYKNCKEFYNDIMLVFDNCILYNGSENDIGQMGIALKQEFLNQVEQTGLKKHLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UL77</accession>
    <name>A0A8S1UL77_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0420074</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UL77"/>
    <dbReference type="OrthoDB" id="288375at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8165164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="186" mass="22801" checksum="DA558D9F06DE7B49" modified="2022-10-12" version="1">MQFFTPKFSFVVHKTFKQKLLARKEKRRFRGLNVYVPEFTGEGSIHPWLDAKRIKLLTKFYEDHRNKHRFTFKLSSDDKKKLNEVMQNYAEIYYLRMLQEKYWLDKHTEVIMNVQKEVNSLPYVLKSELDRKLSEKEMEYYDRPQLEPDSVYFEQRLRTLPEEEALNFEFAQRLFRIAQDKLAQNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UL85</accession>
    <name>A0A8S1UL85_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0420079</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UL85"/>
    <dbReference type="OrthoDB" id="292927at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8165174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="485" mass="57669" checksum="E1D8D62B6E3375C5" modified="2022-10-12" version="1">MQTNYSPPSTPSNIFEKLKHYRKSQMSSVLRNLLNSPPSRPTTMQKPQLKLQSFSQRTSPIPKDLSVFERTQDQTVKHLDFSHLHEFEIRTPNMTPHSPLNSKNQMSREQKMNSFINSQRYNKNDKRYNIVLFFEENSLVFQDIIRRKDAERIVNLFQTPMLLAQQENLTQTFSEMILFLATFFLDCQEYSKATFFLKDCYTLSNLTRNPLLKVKTLLVLAQCAKQYKLFDQQILIVQKALMYTWAFDYHDLEIQCYDAMGIAYFYQGNIQRAEFYHQKWTSGFLESPKSYYRITALEFISMFEKTLPTKAIDFMSSIQGSISIPFINIANGKYYDDKRFIKYNNCNPIEIITTLVAAKEFKEFNLVHHEQTQAMNTTHQRQPKLPKKILEITEKYHKNKELYEFDHKIYDNPKYKLSLQQQVDHRITQSFSLEQVNINIRKFISTQREPFIKAQQIYIRANNIQKEFIPAFVVRYKKMLLQILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ULY1</accession>
    <name>A0A8S1ULY1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0440045</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ULY1"/>
    <dbReference type="OrthoDB" id="308943at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8166248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="108" mass="12714" checksum="04C4E70563D5E13B" modified="2022-10-12" version="1">MYSHSNLKVSSTNKQYSKTKEIDVKSGSNCGCNVWFPQQYIKQKCLIVNKNGYNINLIRMNQNGDFITKQSIDFGHHNIFAQMSEDGEYLMNWDNKSKEIQIRKYHQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UM44</accession>
    <name>A0A8S1UM44_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase C51 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0430268</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8166070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UM44"/>
    <dbReference type="OrthoDB" id="299748at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007921">
        <property type="entry name" value="CHAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051705">
        <property type="entry name" value="Gsp_Synthetase/Amidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30094">
        <property type="entry name" value="BIFUNCTIONAL GLUTATHIONYLSPERMIDINE SYNTHETASE/AMIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30094:SF0">
        <property type="entry name" value="BIFUNCTIONAL GLUTATHIONYLSPERMIDINE SYNTHETASE_AMIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05257">
        <property type="entry name" value="CHAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50911">
        <property type="entry name" value="CHAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035882670" description="Peptidase C51 domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C51" evidence="2">
        <location>
            <begin position="84"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8166070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="264" mass="30819" checksum="BADFC88BD7E035F9" modified="2022-10-12" version="1">MKYIVFILFQLILFSLSEVVVKPIIQCTLQDCEGDDFCYQGYCTPCEIPRNLWFQRKPEWGTKVGEAIQVPAYSNFGDANVLDTNKDEYLKDSKINFGEEIYVGQNYQCVQFARRFWIQNYNTTFGSVNYAEQIFNLQEAYNFDTKKTLQMKKFQNGDINTPKFGDLLIWQKDEGEFPYGHVAVVIAVEIESNYPHVLIAEQNYDQSWDARSFSRALKLVKGKNKEIIISNNRQIFPTKEDWKCRDTEITSQGVILGWVRLNIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UR32</accession>
    <name>A0A8S1UR32_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0470217</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000047">
        <property type="protein sequence ID" value="CAD8167630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UR32"/>
    <dbReference type="OrthoDB" id="10266523at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8167630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="279" mass="33449" checksum="72CAD525A94FC3C8" modified="2022-10-12" version="1">MNLENHNQNNSEPILQNFSSSNFYSEPSNFNQQLFWFQSKEATEILIILASQCQIIFKCVSGKGAKKYQNTDIKEQLPVFKNERNAFVYKIKNCQNDLLRKLEKHLQINLIGDQKEMNFFFFGRNNVTIQTQNPIMDILNPYIHQDFIDTLTELLELRNQVNNTNHYQSRKKNLYNINNHKHFVCWAFISAWQIEIREHLKQLQEKLPTDNQVFLTQGQKSQELIQLKSKSDEIWRKKKQEVEQLEDINQLIMKYQQQSNEKIHDIIKMFEQRSLQNNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UV59</accession>
    <name>A0A8S1UV59_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0500083</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8168970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UV59"/>
    <dbReference type="OrthoDB" id="206452at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd15457">
        <property type="entry name" value="NADAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001967">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050923">
        <property type="entry name" value="Cell_Proc_Reg/RNA_Proc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012816">
        <property type="entry name" value="NADAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23308">
        <property type="entry name" value="NUCLEAR INHIBITOR OF PROTEIN PHOSPHATASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08719">
        <property type="entry name" value="NADAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="FHA" evidence="4">
        <location>
            <begin position="222"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="345"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="447"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="3"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="305"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="447"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="477"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="498"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8168970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="522" mass="61660" checksum="4507C8BBC0817681" modified="2022-10-12" version="1">MSLFAQMEKNAQFQAELQRYEEQADFLFRKQRMERIESFTDQFEFLANEYPCAVYYQGLIYNSVYAAYQSARTQDEEMRQKFVNAQSVQEVYQIAQHIDDPPHWTKQRLRIMEVLLRDKFRRNADIRDSLRATNNYQLIHTFTHSTPSNQYWGVVDGKGQNQIGRLLEQIRTDINNYEDQDKWLLMTFPIEQESKMIPKVELKVLKEGENLPKIEMKHSTFLYFGSHQRNHVHLAHPSISRRHAAIFVNNQSQICLVDLGSKGGTFHNEQFVEPHLPVVLANEDKIKFAKSTRIYQVLISYDDCKKWLKDKIDKLRNDVRQLERINKGKMSKEELKATLSGAIEDTILIKNLPEKASHLELMELFRKEGKIKELKIPIDRQTGNTRNVAFVRYENDRDAKRLISDRNKVFEYKNEKLRIKLVDYAYVEKFQEELRWGITEQLPQPIQQQQQQIQQPTQSIQPTVTKPVEIKQKVPESSSSSDSDSDSSSDSSSDSSRSRSKRKKHKKHKHKKDRHHKKHHKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UWS1</accession>
    <name>A0A8S1UWS1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0480137</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000048">
        <property type="protein sequence ID" value="CAD8167999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UWS1"/>
    <dbReference type="OrthoDB" id="285994at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8167999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="204" mass="23654" checksum="705CD1C0CAA8A43A" modified="2022-10-12" version="1">MKLRSISTIDHPKEFLFPIEPDLSASMTVQNSKIYQMYQKNKQATPNKHPLQAQFNFTNTSRLENNNSISPLRLYPEKTELINLSSKRSLTPTSQPQQIKVKGLTEKFNETQLKRTLRHQGVGIVYCRQNYNKINNKGDGTGEIMLEGIQNLHDVKKRLKTFGLELENEGIQHGVKHMISEKFAKILQTERNKTSRNQHNYFRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V1W1</accession>
    <name>A0A8S1V1W1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0510244</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000051">
        <property type="protein sequence ID" value="CAD8169779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V1W1"/>
    <dbReference type="OrthoDB" id="286901at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="316"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="347"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="436"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="470"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="589"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8169779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="614" mass="71938" checksum="B798C3735151A52F" modified="2022-10-12" version="1">MSDDQIANELDDVEKNQVKVKFPQKSWISMIQNKIISVNQISQSEFEEKHDTNIGITPGRSERESFCKSKHGSSIFFREDLGRSRISDFDPTSIKYRTQPHEVHFRIKVFIKYLVLRILFSIIGPLILVYGLFNKEWISFMSNFKIYGCSKGTAFNYFRWLSNYGFFFSFVYYIGNYGLKKMHYEIGFFCILFITENVTYAIKYAFFHPSKLNLIERFDMSIIGDLCEQSINPYHWAQQTPSMINREFEQSMKRLQIEPTSFNLYFMVAPNPERKKRIQVGYPDKGNKLYEDIAANCISMAREIVEEYNKKCQIKFINKFSIFLALLRVVAYVFIMNFCNESVKDYVIAGQMCIFQFMYFYMITLLMMITYRDLRRKVFAMTQLSHLISAEKVEVYQESKMFPTLNLLCCVSLYSWVNLRKMILDYGLQYTRRQSFMLSFIMILNAIMMVIMTFLFYVESVTINSILQQTVDFVVIASISIALVLQGARINAHWCIHRGLLRKNLFLIQQLSYQIFKSLDYTFKPEDPVFSAFQKLLETASVESVLQDDQFLNYSLFCIHSIQNVYDDLGHQEKSQPYTVMGIAMTYQLLLQVCISTMGLIGTSGLNFILQVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V2C1</accession>
    <name>A0A8S1V2C1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0540107</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8171091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V2C1"/>
    <dbReference type="OrthoDB" id="305660at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="231"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="254"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="293"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="318"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="84"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8171091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="367" mass="42017" checksum="51EB427746770373" modified="2022-10-12" version="1">MNNNLLIIVFFSAWTLLFLLILFIGYLLDKSYQLASISPQTKSITPSCSLLTINPIMVPNVQKQQAFRKGDEGVNIVINNQGQQNQQVNQENEQVNLDQNVSPKRRSSDKKSPTRSLKKFQTQQTIVKKLNIGTYDGIETLQSTNRKSVRSRPSVVNSERNSVQAQSPGQRIISTPRISSVSNLQISGKLLVDSIYKIPKPLKDYYKCHQISKIFFTHKDGISRTFMTFQIYFRQITCFEICGILLIQTQNLSFYIIIGATCGGYVVLKILDYHLIKKISKGSWVLTLKIINYTFWLSILAVLIFGIIWFEINNLLCIIYYLPALIMEIIVDPIRYLIIKYCLADSKKGQNQTLNQVNVLKVMEQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V2G9</accession>
    <name>A0A8S1V2G9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0540109</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8171095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V2G9"/>
    <dbReference type="OrthoDB" id="1749473at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="98"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8171095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="211" mass="24956" checksum="513E040726FCF0D0" modified="2022-10-12" version="1">MDQIEYVNLEKKKPQIKQNNQRGRQNNRPNKNDRNRRNQRDNQPFKNQRKQQWRNKDQQRQNRPFQSENRIVATGKRRRQLKQRKFTQRPKAPRNIQPNVIIKGLDPKMTEAGLQEACKDFGPMNMCKLDRDNFGQVKPEGIGLIRFTRVEDAKACVEKLDGVTVQIIGENNNEKVISATFVGDSEQVDNNRNRGVLNITKRPIKKRDWRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V4H4</accession>
    <name>A0A8S1V4H4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0570037</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V4H4"/>
    <dbReference type="OrthoDB" id="310629at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="28"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="386"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="706"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="706"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="726"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8172328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="738" mass="87526" checksum="AD2679901A30DAAA" modified="2022-10-12" version="1">MSQQNQFNQIDVGTIISAPSGQYTNRQFKLSKLLGQGSEGSVYEAIPINWCLNQNKVALKFSTFTKDYARQFYKKIIDYQNTYENYNSSNYQASNIIRIYDFFDYSFYFVLVMELGDIDLYKYMEQNKKTLTIQQKYSICLQILQSIVFLHKQKLVHRDIKPENYLFIDQQVKLIDFGLIKQFNQDEKRMTKSVGTKLFQAPELIEGKSDYTMSVDVWAMAFVFYEIISGSQLIDAKTITELETQVKFHITNQSNIYNKIDRLVINDEWKQTIKNMLHPDPNYRISANDAQEKLLKQTIVINPKQNTFIIPTQQQITQNFVKQTPLNGQGIQQISLIQQLNTLLMHLQQPANIIQFQQQKEQSIKFLSNIKDQIIKVQNQLENNPNFVLSTNSSDSSQETQKQNQQICKKEIPPSIKQKLDQQRQLIQEIEEKSVSSQEELDLNENFQKITTELNYLTQITQNFELKDQDYKLTQKKVDDLRQKQKLFMKKEILEQNLKELQDQCKNSNEIQLQKDIENNQIKILKLEQQINTISYLQTQKLKQQEKIEQLNQEINILQIQEQEVIKQKDEILELKVKHEYLKLIQNELEKNKQYIDSLNQEIERLQICQSQSQQQQKQIQELVKELKQLEDIEADVNEKKNEILQMKAKLRELPIEREKQAVYSQDIEKLKNELKNFETIQNNIQSLKQQIKQLNQQKNLKLEMQDEETKLKQEKEQYEKEQKKFPTNTVRPSGRNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V5G7</accession>
    <name>A0A8S1V5G7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0560037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000056">
        <property type="protein sequence ID" value="CAD8172004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="298195at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1287"/>
            <end position="1312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8172004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1333" mass="155579" checksum="599CF942C50AC20A" modified="2022-10-12" version="1">MQVNQITIYSSTFERIEKDLGQLVQGENVEYSLNGISDYFDIVQIMNPQTADYTTEDLTNLTFIDLHTMKNGFYHENSRALLFKKYSIYQVRYPSIDKVFYSTIQKCQFVRIIRNNVILLAYETKIWIAKTNEQLYTDSQILDIEVENGFILVTTLNKVMILYYDYTLKETGTIIDSSLLNNLLETNNTYSIIQTKYALNNLLLLDSTRGVIVLKVHTINEFEFDYIYSSPQESNYYSFCILKDLNSYIIAISYNTNNLILFNLHSQDLQSWTLQDHDLLRIKMEFIGELGRQQFLCLFNSTYLMIHQINPSYIIKTITFQSPMIITFNVYKSQIYIVQNTKQQSFIISNGLLIMKLNNQANYELQNLLLIATELNQCAVTINYVIYNYLDNKLYQKTNLQHQIKQLINYPTDPQVPIFKEIGVSGSNIKAQIELQDESITLKLGYIEDIKIKGIVWSDSNDLPNDSVYVDICEVQQYVYYLIQSKTLKAILYQCSISNPYTELNCQKVHQFYPNRILSKDSFQWIHNNYVIFGYISKFSHTLELYQLNQSIVQELFTIATSLKDEKSEITSFTLSKTHVFVVQQRKQQIDIISLTSESIIDTITTFDVPSYQFNPKTVQIQSNLLIITGYQNIIIGYFFNSFRLMDCITFSDSESLATGVTEYTFFVAIDYGYKQEIREYSTRMQKINLLKIIPLFHYILHKPFEIMSQMNLVLLKAYTPVLQQTVLLIYQPDVELRECLVISAPLGWYAEKELNLGVTAFHQSGLLIHAGSLMQFKNFFVAKDFQAVITSKYDSKYIHRKELNISYLTIDSPGIIEQVQNVTFLNLMTNIEAVSSHNKLYLESKIVPIPNNWFKGLVIDYKIECQQCNDKIILTPKVHQISNVHHYEDILSYCQLNETLQVILTNNSLMFLDHQHYVKHQPFFFDFETSYSPQNVWCKDPIILITGSSNTKLFWNSFIAMIIKEGNSFRLVDQPVIIKIFRKILQLEILENFNFILLEAIDEEEQSSLCSWHINYASPSFLSFSKMSCVDKSKQPNFMVRSFITYSFGNLVRVLMNELNYGIYVQDFQYQNNFIKSLIVINDVIRISPKSNLEALIGEKINEGITYDKILKCGEEANKNYQIFNVILLTREIAHLKMIVRFEDELFKEIIITQLIQRYSDFKLMFPSSIVKDYLALAYKKESTRQYSICIYKLDINTEIILQFGGIEITESYSQFFSLLDDENGQLLLQLQLSNKNLSLYKVEEYATLTFLEEAHEQQFVLIASNLVSSASMIVQSFDILQSSSITYWILLILLLLAITILSLMFFVIYWKNRQKKQRKRMSLQQQSLILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VGR1</accession>
    <name>A0A8S1VGR1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mei2-like C-terminal RNA recognition motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0650088</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000065">
        <property type="protein sequence ID" value="CAD8176450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VGR1"/>
    <dbReference type="OrthoDB" id="417481at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12531">
        <property type="entry name" value="RRM3_MEI2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034454">
        <property type="entry name" value="MEI2-like_RRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007201">
        <property type="entry name" value="Mei2-like_Rrm_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23189">
        <property type="entry name" value="RNA RECOGNITION MOTIF-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04059">
        <property type="entry name" value="RRM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Mei2-like C-terminal RNA recognition motif" evidence="2">
        <location>
            <begin position="168"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8176450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="298" mass="35203" checksum="E4F5C6E61D7A350E" modified="2022-10-12" version="1">MDKQIRLNQEQILQCIRNHRDLNEILSKQKDHSSQELFDEIERVTNNMSNESKESQESKSNSDTIDRAIKMFAEPKVILYKKENSLGSDQIMEQISQVFNNKSFDQKVGSNLSTEISNEPCLQLGSSRQSLLTSGSETERRKRKTISEEESHYFVVRLEDVINYSDERTTIMIKNIPNKYTVQMLQDLIDLKHHDSYDFLYLPIDFKNKCNMGYAFINFIHPLYIVQFYKDFHDNGWPHFNSEKICELRYARIQGRQALVQHFQFSSVMNQKDKKLKPVIVPQSELSRIHQLIQRQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VH75</accession>
    <name>A0A8S1VH75_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dynein light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0650140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the dynein light chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000065">
        <property type="protein sequence ID" value="CAD8176554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VH75"/>
    <dbReference type="OrthoDB" id="10033309at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21450">
        <property type="entry name" value="DLC-like_DYNLL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.740.10:FF:000018">
        <property type="entry name" value="Dynein light chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001372">
        <property type="entry name" value="Dynein_light_chain_typ-1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886">
        <property type="entry name" value="DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886:SF35">
        <property type="entry name" value="DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01221">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01375">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="1" id="KW-0243">Dynein</keyword>
    <keyword evidence="1" id="KW-0493">Microtubule</keyword>
    <keyword evidence="1" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8176554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="101" mass="12093" checksum="FE863200E12D4590" modified="2022-10-12" version="1">MSSQTTNQYRMYLGARVLWPPDCPDDILEGAINETQSCLKTFEAKDGQKMAEHLKKYLDSNFEPYWHVFFGKNFGCHSIHEKRRFIYFYIDKTAYLFYKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VLP7</accession>
    <name>A0A8S1VLP7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0680096</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000068">
        <property type="protein sequence ID" value="CAD8177807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VLP7"/>
    <dbReference type="OrthoDB" id="668540at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010608">
        <property type="term" value="P:post-transcriptional regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF12">
        <property type="entry name" value="MATERNAL PROTEIN PUMILIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PUM-HD" evidence="3">
        <location>
            <begin position="160"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="188"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="224"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="260"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="298"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="334"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="403"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="446"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8177807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="517" mass="60083" checksum="1F598D3C37E832A7" modified="2022-10-12" version="1">MQTKEYEAEDDLLGSILRNDDYEEVFSNNSSPLCNQGFKSDSIIENVSTSEKHYFDSFNRSFEQMSLGSDQAQSPMSNKAVYSQYPQNHQKFSRQDDRQSVSISFDGHSFSTQESSINYRKNGNEHFGYLNKHHNRQVDYQVPYSPGVLRPNGIYQPGFNYQQPLNGLGFTGYQQERQPLHDINDDIQLTNDLDNMCGNQMLSRKVQKIIEHGRPDQRELIFNKLERSCVQFSKDVFGNYVMQKIFEKGTPIQQLRLFNKIRPHAYELSKNNFGCRVIQKIIEIISNNENLQDQFIDSIRLQVKSLLYDSSGNYVILKCLEVLKKEKIEFFLQPIEDSTLFLCSSQYGCKILLKALELYSPIQTDKIMTTCLQHQQNLCQQEFGNHILQFAIKNTHYQPFVVNFVLQHFEILCMNKYASNTVEKVVEASTNEVKQRIISILISFSQINGLVTFVNLSINPFGNYVIKKMIIMSNQNILQPLIDILRQDQYLITAIRSSDFGQRIYQLIEKTLLMYKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VN44</accession>
    <name>A0A8S1VN44_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0700012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000070">
        <property type="protein sequence ID" value="CAD8178600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VN44"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8178600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="119" mass="14256" checksum="4F9EA4519A9868E3" modified="2022-10-12" version="1">MLMTETQQLLFALQNKISNSIRKIIDSNSQFQSRENPLYQLENLDSFEFVKLVFSLLLISKEHQKYELYPDKLKVKIKQYRKSKQKKNCKPKLNSHQQQIYSLKLLKGEKVQYFKSLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VUE3</accession>
    <name>A0A8S1VUE3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0720214</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000072">
        <property type="protein sequence ID" value="CAD8179775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VUE3"/>
    <dbReference type="OrthoDB" id="309408at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="492"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8179775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="813" mass="95588" checksum="157713D73CC767BC" modified="2022-10-12" version="1">MSSDLDKKLCQKHKLEILTIDLKQSTADEDKYLCIKCLMEKIDIQNMALVEETKTMIKQMKSEQLTFKIKEYQRRIEIFKQIQSQVKELKLSINNTIDKVQSNLDQKITLMENELDESESKTFVSTFEEDIRILSKNYKGSFSFEVPKEFEKSIDDNSYIDQIQQQLQSIINCPKLIQIKESLEKTKAENGNSEVKQIQLLNKQEEDPQKTPSLKIQCNKHGKEIIMFNLNPEKTQFSRLACVECIQSNDPIKYTTLEDANFKWNEYRGQTSDQVKRFQNSRYLQSSQIIEILQDIKEKYNSTISEIINKINTQYSMFSQNETNEFNDNMIFLMNTEQINELTEILSQNDKFKALAEKQFNIQKEDFNQLVLISTNFGQLLQNDLLATQKINKIFKDSSLNISNVKDQTDDIVQDDNNSIQNLQQIKQLNQQLQHFKLYQNILDEALNQYDLIMQTISSLSNQFKDVQQQQFNHYVSKIEKDLSLMQKFTQFDQVATEHKLLKQEKEQLQNQLIESEKSNLENQLVINDFKKHDAEQIKQISDLRNQNQGLLVQIKELGQQKTQCELILKEKQAELQICQQSNQKNEQMMKQLQQNLKQEWQFSQFLTFSTTYKHSSCSVTQNGKVIENSSGGYYCYMCDQMIPKNGVIQFAIKIIEINSIMIGIGFRDIVQSKGYQSCYSIGGGTYNIYSSGYCYNHDQQDKNDKKIAFEFQKNDIIIVEVDIQKKYIKWTKQSTNESFTLTIDTSKDLYPCVKVILNRYFSILEIIHLRKNKNNKILRRQLKKLLNFIYFSISLEYHKSFNSKICQFSFFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VVS8</accession>
    <name>A0A8S1VVS8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0690190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VVS8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021258">
        <property type="entry name" value="Epiplasmin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10992">
        <property type="entry name" value="Epiplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="278"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8178426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="309" mass="35030" checksum="13A1F4C0C3860EF1" modified="2022-10-12" version="1">MSQRPPVPQNQAPQPQQYQPAPQYAPQYAPQYAPQYAPQYAPSFAPQYAPVPTYAPQQYAPAPIAPLTYSVARPVAQPVVAQPVVAQPVVTQQIVAQPVVQAPVLQQSVIAQPVVQQSVHATIKGESRIEYIPYQKAVMEYEEQEVVQYVPREKKVTDYYAVEYQTEYVPQVFQEKYTEYVPVDRYQERVEYYPVERQVVHQQVQPVVQQVVQQPVVQQVVQQPVVQQVVQQPVVQQVVQQPLSVVQPVQTVPVTYAPQYAAAPIVSSRVIPSYPQYPSYPQYQQAPQYQPAPQQAQPQQPPRSNLNVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VWF1</accession>
    <name>A0A8S1VWF1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0730159</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000073">
        <property type="protein sequence ID" value="CAD8180109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VWF1"/>
    <dbReference type="OrthoDB" id="295595at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8180109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="264" mass="30418" checksum="45119130C2A18B2D" modified="2022-10-12" version="1">MNQQFDTEDSINSDEAIKSIECKYLTPNFHPNLDDKGTSVHKRMNAINQLRCKFKESLVSKDEVFTQSSPFKSTNVISSQNGLPQSEFPSSTLKAEVLTKQDKVHLADSRKRHNTKNSIEQFQEKQIKQNHRKSQPHLQPNLIQTRKPKRQIFVTQIVNDHCEITSFPFPKPQRLSRIIQNNDGYHSQRGSSTSTPRGILKSGSFEVLRIGSRPSTQSLNSPMMRPSSKKVSFEFTSEQLRKMKNNNGNSDHNKYQRLKTKFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VX56</accession>
    <name>A0A8S1VX56_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0760110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000076">
        <property type="protein sequence ID" value="CAD8181381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VX56"/>
    <dbReference type="OrthoDB" id="306621at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8181381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="395" mass="47186" checksum="71F5FAE5ACD90683" modified="2022-10-12" version="1">MSTQISNQKIKFLGEYCYQPPQNLIQQQNYQNNLPNHQHTQNSNPKISVRQTPYLNNSEPNKARSSSQFQVPLNPIHNTFLNLNEMNLYEKTYEKSINKPQIKDEYLNTQYSQLKKNIRDNDQQSINCQSYVYKINDGHNNSDNTNSQYQNQPQIYNFIQNRNNNTNNQTNQKSYSQDINQQFLQTNQKNNSEFNCQQNSTNSQLNYYNNNQYQVQNYPTGNQFIKNQGHISIVQASIEQKQQQQNQQVQQDDNFEVSEDLTQSITRSISYSCKHCQARIFHEIIQLDCYHFYHPQCFQDHLDKQIDVQTYRPLKCHCQKKISESQIKNVLKLNQKSDLIDKLQKKQISTIYKMYNQILKLQKCNQCDFFWVADQNDNFQKTYYCQQCEKNYYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VXY4</accession>
    <name>A0A8S1VXY4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4200 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0790169</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000079">
        <property type="protein sequence ID" value="CAD8182848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VXY4"/>
    <dbReference type="OrthoDB" id="298534at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051147">
        <property type="entry name" value="CFAP_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025252">
        <property type="entry name" value="DUF4200"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21683:SF3">
        <property type="entry name" value="CILIA AND FLAGELLA ASSOCIATED PROTEIN 100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21683">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 42 LIKE-2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13863">
        <property type="entry name" value="DUF4200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4200" evidence="4">
        <location>
            <begin position="115"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="461"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="132"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="305"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8182848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="510" mass="61413" checksum="8FAA133053632CC8" modified="2022-10-12" version="1">MSEKLPQIHKVNKSDIQSPFRYPSDEEVFLFREQDRKKQEAQNHDEMRIWDKKTATNRTQLKHFKSYETQIDDKFKSKTAYNDKDKKIINEALQIIQERKKQRAKGVSKEPIVTLLEQKKEMFLVSKAHGIIEEEIDKLKLMSKEKKEALEQSLKMLETDNENFQKYLDTNKQQKLDAEQKADQEVKEKKAKESEIKSINIKITSLRAEKTRNEEAVNNYMDHKKFLDKLAPKEWHDAKEKKRQQLVNRLKEELIKSKGLTEKQFQQMVDNKKLDELEEFEDEYEMYFKQPSQLVEIFNDLEERNLFLIQTTQDAEQNLEELKTKFNKIKKSLDDKVFTLQQNKEQLKKNYENVCQQIKTLQLKGTEKNAKKDHKELRKQIIEVCENFPNEIQGVHDLQNKETLDILVQIELHLERQLKQIRSFRPDRVENFRQECERDWKVKQRDSNNLKLKQEEERRIKANQEKIMQPSKKKTGRQPMFRSKPFDRVIVQQEESVNNEELEDQKYFME</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W0G2</accession>
    <name>A0A8S1W0G2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0770019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000077">
        <property type="protein sequence ID" value="CAD8181682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W0G2"/>
    <dbReference type="OrthoDB" id="10498858at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="87"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="300"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8181682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="640" mass="75048" checksum="3CC244AB5BA40349" modified="2022-10-12" version="1">MNQQKKIKIQIQPQNIPNLNSITFQFTQQESLLKQAIQFILDQNEEIDQQNIICYSKQKKRVIYEQETVDSIIDLDEKPEIELYLFNDILNRLASKQEQKIEIINQQSNNDSKTQNTKIEMTALNKQIEELLQERNEFREKCSLLEEKLRNLKKQQQEKPNFQNDLQNSTNLESYRLKLELEQSIKSTTQLKDIKQKQEKLLEELEKKLNQKQDQLTQEIQKNTSLQQANINALRDQQILEGKFQNLREELKKTNYQQEKLQKEVDQKSKILQDTQVILDQFKQSTHSKDELIIVRNNIISEKEKKINQLSEEIILLKKDKEKLQSDYTSLTVDNQEKLIKLKQLEKVDNNLKFISYFIENNPFKQLNDLFITPIDAMYRKKLNPYEFEKQQNANTNICDATVKKLSIESGQNVLQWLQFKSDKSINEFIKKNPQKMLKVLKSINKDGQGTKSKCFLMQIQQIYHNDKSLEKKVFALKIIDGHEGITKEEHAIFLAYQIIVAQVLAECFQQKITKPPFKFEFQLPHLFTIGDNIYLGQELLNQDYKSFQQNVDNSIIKLFNAFSQFTSDFTSGALIVTNFKGGQLVGDKYILFEPIIHTPGGIFGPEDQGQIGFDLVLQSLRENPELNGQEYLKGLNYNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W3K0</accession>
    <name>A0A8S1W3K0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0740244</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000074">
        <property type="protein sequence ID" value="CAD8180676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W3K0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8180676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="76" mass="9086" checksum="9C02EB09E25AF854" modified="2022-10-12" version="1">MLNIFVTRLLIKGIYNYLIWEMQYITPSDEKITMIFDDDKNILCSSLQFSLINKIIINMRRLKQFSEQCPGYIENC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W7L0</accession>
    <name>A0A8S1W7L0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0860223</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000086">
        <property type="protein sequence ID" value="CAD8186228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W7L0"/>
    <dbReference type="OrthoDB" id="310744at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010971">
        <property type="term" value="P:positive regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110032">
        <property type="term" value="P:positive regulation of G2/MI transition of meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000071">
        <property type="entry name" value="Rodhanase family domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828">
        <property type="entry name" value="M-PHASE INDUCER PHOSPHATASE DUAL SPECIFICITY PHOSPHATASE CDC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828:SF17">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="126"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8186228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="268" mass="31481" checksum="0E57E5AFDB4EC734" modified="2022-10-12" version="1">MNCSSNEMPNFLPLFKTCASLSSFDCDSNHSTKRFGDELLLLSPTRSQQLSNPNKKIVIGDDSWLRRGISISSETSSPDRQFERQEFFSSFRVPVNFCEENDGLNRLQHFEKDAVNYITSETLYKNANDITLFDCRYKFEFDGGHIHGATHLCNPFEIESIFFKQIPTVKPVIVLYCEFSEKRSVQIYKQIRNTDRNLNQYPNLHYPDLYILNKGFSNYSLEYPSQIQGKYIRMDSKEYSTQYKEQKCQTDNQWNSLKLKKRLQKINI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W982</accession>
    <name>A0A8S1W982_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0860089</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000086">
        <property type="protein sequence ID" value="CAD8185961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W982"/>
    <dbReference type="OrthoDB" id="307457at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="118"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="279"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8185961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="442" mass="52161" checksum="B65D599368EA4244" modified="2022-10-12" version="1">MIASKIIKRQEQLTSNPIQDVSQMKKVQSNLMMVSKASSLPKQFDKNEKLKTTGNKMESFKHSKNNSMNHLSTTENTKIIDSIQNVSSIYINNKTEEIQLRNEIQLKAQIIADLNKHVSQLQQQNTNNEMIISQLEKDVQQLNQTIEKMCEQLTDSNNDYNKEIQKFQDQIINKDKIIKFFKAENQQLLTIIKQGSYDKKKVDKENQPNITQANQQDQINYLKDQIDLQRKQFQQTEDSLQKQITGLNQFVNELFQQQSSDRLNTNISSRNDKDNRNRKLSLQNDMDTFQSQLQQLDKQKHKLDHKFDNIQCNLNQLTEKQLNYVCTDNTQSMKEIIKLKQIVQIQKKQIELQQKTILNQHFEIQNLQTLLYKNDQYSISKTKEIKENNEDIITNEFTNSPKDDIYHLNDSQSEIKPKYYMDSIQNTFQKFQVITEESTFRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WI06</accession>
    <name>A0A8S1WI06_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Choline transporter-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0910083</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Choline transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the CTL (choline transporter-like) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000091">
        <property type="protein sequence ID" value="CAD8188325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WI06"/>
    <dbReference type="OrthoDB" id="292257at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007603">
        <property type="entry name" value="Choline_transptr-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE (SLC FAMILY 44)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385:SF14">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04515">
        <property type="entry name" value="Choline_transpo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="220"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="265"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="310"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="455"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="524"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="547"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8188325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="622" mass="71570" checksum="34590EDB43816AB5" modified="2022-10-12" version="1">MFQRRSEAREEQGETVIALYSQQTNMSYIPPVYLKNGPNQNRSCTNLCCCLLFLLSIGALGYFYFLTYSSQHIERLYTPVDSEGRECGQGNLNKFPFIYFVTPEEKYLYRTVCVEKCPKKVDQQLLCAPNKVVIECHNNPSPTNPEKRVLVYESHSYSGNICLPTDDNLKNAVQPAILVGEIQQALVNTKANLPIIAMSGGAAFLLAFFFTIMIGLCTTFIVYVFILVYISLTGFLSAYFLLYFIKLPLDLFPYFDKLTYAWSPYMLGASITFGLLCIYAIFTLCWNLRKMKFMISLVKLATTFLYQNKTIIIVPIIFFFLVMSTLLIWIASALAVLSEQQIDYSSDQQIYPFEKIQLKFDTLIKLMITVLALIWLMQYILSLARFLNASTATLWYFHASSNKGFVYDSFKLAVRYHNGSITAEAIYSFFFSGISKAFNLIYDSLNRCRLKRHNIILNCFASISLCLCCICENFIMYINNYAFVYIVMTSADYFESSKAVHFTIKRNYQDFETLSGLGEQFTHYSKMFIFLVTTLGTFIYVKEQTDFTLQLYTFAIIAFITLSIATLFMDMFGQSADALVLTYFTDCEVQKYHFGLEECGSCPSQIRDQVQHIREKQKIYYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WI34</accession>
    <name>A0A8S1WI34_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein kinase TOR</fullName>
            <ecNumber evidence="8">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0910098</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the PI3/PI4-kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDO010000091">
        <property type="protein sequence ID" value="CAD8188355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="309331at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016242">
        <property type="term" value="P:negative regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05169">
        <property type="entry name" value="PIKKc_TOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000057">
        <property type="entry name" value="PIKK family atypical protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000006">
        <property type="entry name" value="Serine/threonine-protein kinase TOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050517">
        <property type="entry name" value="DDR_Repair_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009076">
        <property type="entry name" value="FRB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057564">
        <property type="entry name" value="HEAT_ATR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024585">
        <property type="entry name" value="mTOR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003151">
        <property type="entry name" value="PIK-rel_kinase_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026683">
        <property type="entry name" value="TOR_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139">
        <property type="entry name" value="ATAXIA TELANGIECTASIA MUTATED ATM -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139:SF9">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02259">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02260">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08771">
        <property type="entry name" value="FRB_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23593">
        <property type="entry name" value="HEAT_ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11865">
        <property type="entry name" value="mTOR_dom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01346">
        <property type="entry name" value="DUF3385"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01343">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01345">
        <property type="entry name" value="Rapamycin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAT" evidence="10">
        <location>
            <begin position="1338"/>
            <end position="1889"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="9">
        <location>
            <begin position="2063"/>
            <end position="2376"/>
        </location>
    </feature>
    <feature type="domain" description="FATC" evidence="11">
        <location>
            <begin position="2452"/>
            <end position="2484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8188355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2484" mass="288597" checksum="D08B8D4659D6DE19" modified="2022-10-12" version="1">MQKPELQRIFKDLAAKVPNEQKEKAAMELQSVYYKYIQQSDELFNKIQKLINSSEIHEKFGGILALDQLTTTVQEAQVLTFVNKFIPNVSGQFIYNDEKFLRKSAEVFGKLLRLGGTKIAQVVDLHFAEAQKQLRSEKYKLAGVLCLKEILNEAASITFNKLFQTMQTDRNFTLIHGAIRSKQQSLREAALDLFSEIVKQIAQREQSYQDYLVKIYIDEIESKKKSKEEEYIHGNIMMIKILLTYAKQDVFSQNQIFDQGEYVLSKKDHKLAIINKAVIETLPILAKHAKHHAVQEFMEQSISFLLQQTMQARSVKDKGLPYMTLAMLISFLNENMLQDLIKKVILHIRQELLQKNFCVEMINCLQVIFQNYTRKFAEFTSVDVLVDQILLNGLHPQSVQFLNQLCRSQPRQSEYIQQKLLQTIAAILLKKIINFVNSKQQIDNSILNDFQGYLQKAITTTEFRSPEAIANAIQTLSTFSFDLQDSLAIFVKDAVLPNLANSNPIIRKATAKAGCLLYIKKGRSTGQQMISKNVMYEILDKFMNVAISDTEQDIRQTMLASLNENFDPYLNSPNNLRKLFLCMNDPIPEVQEIALTILCRLSILNPSEITPFLKKTLFEYLQTLTFDSNQPEKQTINKLYLLTSLIKHGRTIVQPYTSNIAKVIQQHLKNPNTSAIVTSYLLQAFAQLTETANQEILVNLKEVFDIIITAMQDKSSTLKREAAVKSLNLIIKNTGFVVLPYYRFPNLMDVIFQLIRTETIPEMRQECLKLLGNLGAVDSFIYKSVQGVPTKQKFKFLKNYQNALNSVSSSELMNEVRGYSEDLILIGNFSTTMFLHLGKLKMQTDQDTLLYQNSGALIIKSITLDAQSHYHQAINSQDIEELFQQVPVIQLNDIDYYSKVTLKTLLQILLDPSLSNNHELALETIMWIIGTLKAKTASYLNYLIPVFARLLQRNEEMREKVLTSLQKVIHLCGIQFNPQYIDQVIACVMLFCQGQESSKLVLIGLEIMETLIKSSKQHLRHKVEPLVRLINQEISQFEEEKELVRKGIKIYILLENLLDSQLHMFIPFMCKLLSKEVSSVLLEVRKDIINLFVSLSRKCPTTVQYLSLIVNSLLNLVELSAKTQQQLEMHQTVLNCIVNLILQHKNLMLVYLPMIHLQVQKYKIHHQQYQKLVEIFLMYGNLEDLNNLLDEDCKAIEQLFPSQITSYYSIEPNAPMYKKIEPDDLVAKFDTEQRKLKEEWLEWMRNTSVELLKLSPFLVLSPCSSIAEMYQTLAYELFNIAFDSAWYFLNDKHKELMVQYLVRVIKAENIPLQISQTILNLAEFMQHDKEGLQIDISSLGELAEKCMAYAKALYYREHEFETASLKAIQSLISLYTNLGLQESANGLLTYAKQSLKIQVQNTDYERLKKWDEALQEYRQQQLKHENDQRMDLAIKLVVPKMRCLNALMQWQTLISQAEEIFRSNEDAKQKEIAHLAANAAMHLGQWDKLATYNEQVKAEELDKPFWKAAVCISKGQLDEAKIQVRKSRERLDGLVTGLLQESYDRAQDGVLKLQQLVEMEEIIEIKQFENKVQKAAQENVGEYSYIKLLDELEIRKKKLKDIWHDRLSGAPKDIDVWYRLLSTRQLFLPKVHDLDIWIKFAKLCLKRQKMVLCKSTIEILKEQFQNQIQAPLPVTLHIFNMQFEYVHAKHEIQILDQVREYFTNQEQISSIDSKLKAKTFFTLGKWAYERAESTSDLEQITKQFDESLQYNSTYAKAWHYYGLCNFEVVEQQENRQSMNAHVFAAVKGFLKSISLGSRDIKKGRYILQDTLRLLSLIFKYGMETAITDEFRQNYKQIDVIAWIDVIPQILARIQIQNPIIQQLLQDLLIHISRIHPQALIYPLTVACKSKNQIKRLQVLKILDDMKKHSPILVNEALIISEELNRTAILLKEAWREGIQEAWTSFSQDKNKTHVERILRGLHDNMRVKLESLSEISFHQTYGQEIFEAEAWLQRYLRTEDQVCLCVAFDIYTRIYHKVQKSLEKMKKVHLENVSPKLLATQNCEISIPGLYKPNKQLITINGFAPRLDVLSSKQHPRQVKMFGNDSKEYHFLLKGHEDLRQDERVMQLFSLVNRLLTNDTETERKDLTITRYSVIPLSHNTGLLGWVQNCDTLQQLVREYRDKYAIRPNAESTLMEQFCSQYQNLPLPNKVEIYRHILENTRGEDLQKVLWIKSPNSEVWLERRINYTRSLATMSMVGYILGLGDRHPSNFMLQRLTGKIVHIDFGDCFEVAMKREKYPERVPFRLTRMLVKAMEACGIEGVYRHTCNVVMRVLRENKESLLAVLDSFVYDPLLTWRLLNAQDHKPKKEARNVDLHKQIPQQMINQLNDKEINQGKSLIQVIPQAKRRGSIIDDGKQQQTLKRKESGREKEIYFEFADEEREIPDDLQNQKGLEVIERIKKKLQGRDFKEHEVLSTESQVNQLILQATNHENIAQAYLGWCPFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WM29</accession>
    <name>A0A8S1WM29_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0940191</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Plays a fundamental role in microtubule organizing center structure and function. Component of the infraciliary lattice (ICL) and the ciliary basal bodies.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the centrin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WM29"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000077">
        <property type="entry name" value="Centrin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000070">
        <property type="entry name" value="Centrin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF59">
        <property type="entry name" value="EF-HAND SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="25"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="61"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="98"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="134"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8189767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="169" mass="19841" checksum="F500D2A83763AFD8" modified="2022-10-12" version="1">MNRNKQQPQRQTAPQRKARSELNEEQKQEIKEAFDLFDTDGTGYIDVKELKVAMRALGFETKKEEVKQMIKEVDREGKGVIEFPDFLELMTVKMGERDPREEMLKAFRLFDDDNTGKITLRNLKRVARELGETMTDDELQEMIDEADRDGDGEISEEDFIRIMKKTNLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WNX2</accession>
    <name>A0A8S1WNX2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0990182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000099">
        <property type="protein sequence ID" value="CAD8191714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WNX2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="276"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="344"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8191714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="431" mass="52007" checksum="1B0E36CC8605CC37" modified="2022-10-12" version="1">MQFMLFKTDQCLLLEISIFTKHLMKLSINFIYIKNQDKIQSSLTNLLIMKRKVWLSEKFYQQIILKLKQFIQNNDAIRDTSNLRNFYGDEGFFKTDIQGYVYKAQRMTQNIGEIKIQYQQEGIHANVKELVEKTNQNANQDQKNSTSIQPECKYNYTMMYQILTKEDSVIQLQAKMSTYLKNQIKKLNHLKEYFLSQPLHYQVIIYYKHSNLDYQSIFIKILIYIKNELIQILCKKSQFCEMLTLKGKHCQLIYFNHLFLTTISSYDHYLSHNYQYYFLTLFLTVILKVYLLTKIFVTIFILLYKLFIFILKSRYKSIHFNISFISKNFLSQNYFSFSHIKASLFYNYILCLPIAIPIYQFQYWKIEQIENFTNKQSCYLIKFLSHTILHIQLNKFSNLFNIIETLIKQQQMYQIIQFQSKYLDLQLSTLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WST0</accession>
    <name>A0A8S1WST0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1000019</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000100">
        <property type="protein sequence ID" value="CAD8191807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WST0"/>
    <dbReference type="OrthoDB" id="308266at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071203">
        <property type="term" value="C:WASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028191">
        <property type="entry name" value="WASH-4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028283">
        <property type="entry name" value="WASH-7_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028282">
        <property type="entry name" value="WASH-7_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027307">
        <property type="entry name" value="WASH7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31409">
        <property type="entry name" value="WASH COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31409:SF0">
        <property type="entry name" value="WASH COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14745">
        <property type="entry name" value="WASH-4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14746">
        <property type="entry name" value="WASH-7_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14744">
        <property type="entry name" value="WASH-7_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WASH complex subunit 4 N-terminal" evidence="3">
        <location>
            <begin position="82"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="domain" description="WASH complex subunit 7 central" evidence="2">
        <location>
            <begin position="648"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="domain" description="WASH complex subunit 7 C-terminal" evidence="4">
        <location>
            <begin position="1012"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8191807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1186" mass="139431" checksum="95B9EDC9DCD92F14" modified="2022-10-12" version="1">MKKNRFQQQKEEQKKQNYFDEEEDIDLEDDGLFGKEVLTASKPYEEYFQKFNELYNNHIVNFSAFEEDYQIPLTQPDYESIIQSHFMVYEKNMGLFDFMKSDNKVFDKISASFVFIDNQIRQIEDQFYKFLDKLTIYGELADLHLEEERKLTDGEAEIMICRMLGQFKQIYDIIKKLINYITHLVNQLNLLYNKKDPISKIIKIFNLNFPLDTIGRALQLIYVLDNVVEQNDYLQEHWNYCKRMLKIVKPDPTKFNSNAQNVKQLEKIMIRLDKTVLSGSCLQSVLAQNWDQNVHEKTGKEKNANIPAIKNNKEFFNVLNTYLKERYELFDKTLGTAKEMFERDLLLPTFCVYALVRQLYQNDENRDLWKNLWSLQKKVPMVDGHSQVLCYLSKFLMEVTPLTKKSQTMDPKDPIANFKNYLQRLSGSFQSSLTAQYVAFTTWIVRVDSFSCSQASFNNIDKIDRERAQKYIEERMNQRVILVLQGLTIASQTRNLLQALLLGHFGLEEQSLDTSLSKDVVLAVEMLKAIEYYFEQKYDVFQPALIQNHITKNGQRIIQQVLEKMKANLKSFGTGGAEILGALQILNSILKSTLSPLKLQTMYFLMDFMVAKDIFTNQEKEDFRNILWKLDKLILLPYYVKKVTASTYFYWCRELLPSFFQYIYQHPSQAKRFQFLLNAVSDCSVQLKDCMHLENPDELFDSFRQYVVKEFYAQYLQPLARDIEDYLRIQVHTVLIEKITALNPFKQQVKDLKRLLDIDYVLIFDSIVNLRTEINQILNETFYNINVLNMYDQETYEQMRSLANSKFGLNLINVYLPTQTVDQGNFDVLNVLKNIQQFFTKYTYNLYQQQFVQVTSESKQVYSISIQQIADSIRTHGIGILSTTVNSVYKFLQKKIQMFSQFLFDDLIQSPLVREDRFFREKKDELDGQYPYDRAEALYKEIKKMGTFDDGSNYLDKFRTLITQIGNSLAFVRLLRSAALHVNSKCLEYIPSQYNPTQLESLTREAGFSDATVSTAKELEEIFTLYKSSFSEKIDYINLLLKAFSSINTPENEHLSLFYLIVPTLTLNFIEKMLISKDQIGKKNSTTEVFISDDGFTLGIQFFLTLLDQKAQFDSLHWRKEIKNKFTLDMKSAKTLATTKPNQSQRATDEINMQKELSLRKLNMQYDEFQMFFYAFNSCKILFLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WU26</accession>
    <name>A0A8S1WU26_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1000076</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000100">
        <property type="protein sequence ID" value="CAD8191905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WU26"/>
    <dbReference type="OrthoDB" id="415460at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="343"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="387"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="444"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="488"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="521"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="7">
        <location>
            <begin position="627"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="157"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8191905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1246" mass="145510" checksum="CF79BE91BB841F9E" modified="2022-10-12" version="1">MINRDAGFQDLESGIQSNNISSGQLFDIDQPSPIGGYKLDPTLEFSQLQQLEALKLKRDIFDNNHKQAINLGHKADVNEEDCLISNLSGKRSEPQGSISSISCNLKIQKPLTVKTTQNKSNKSIQIQQKFDQDDFIIPSPIENNGTINLGLKSDAKIAKYQTFVQQLEEEQEQNERTKRAQVNRDTVKQSSLIQRGRSKEMKTGLNEVWTQKAFIIIRLVSRFIQQLKTKTERIKFRLITKRIFEVICDNSANFEYMLINRLIKQKPSISLIIFDQIQNKALKLLNSIEAINAFFGKYVKVIKPDSLFKIVWDIILLLFIVVNIFYIPIYISFDVRSSGVFEWFFDLLPSWIFIAEILLNFNTAYYDKGLMHEDRKQIIKHYVKGNFFWDLIVIIPFLISYMDIPFVRYTLLLRLTRLSPLMTSIEEVLNLEDNMQVFLDLLKLIFFLLLTGHFCGCAWHWVAVIEYQNYGEELTWLTKYAPDAMDYYWFDRYIISLYWSVITTVTVGYGDIVPVTTVERIFVIVVTLLICGVFGYCLSNIGNIFKQISDKKAIYKQRIREINQHIRKRGMSYNLQLKVKKYFEYFFKIKQEEDQHAEQFIEQLTKHLREEVLIDIYSKTLTQSRFLRENFSDETIHRLCQIVKETKLYPEQLLFQRNDSPKSLWFVLSGAVEYVADHQNEDEHFYTETFLKKITQGAVIGEREFISQTPYEYNARATKFTQLLVVDFQQFYLIIQENIEEFERYSMVKDNLIFNSNYKAFGQICEICGWTHRFIQCPFVFLQPNKNKIANSFASIKTNRRLTFPYRTLPKQNWKNNLPDVQEAALGYIVLNNIIPEKEINDAYLINLGFELNDKDEDPQIQVQNKKNSMFKDQKVLSQFENNLQQQPVPQPSIQTVLPADQKSQNQTAIFLKDGQRASMTNESQIDWDQGSQFRRSLNRIKLQGIDKGKTLQSIKGKFHQNGVTDLLDESMIQEEVKIHNANVGSQNFIHSKLKIVKNKINKNQNQIGIRKVSWLEFDSAINQHLDNKPIHLLKQVSKGSETNFDQSLPSCSQEIEIKQRIEARENSIISKKTSNVEVTRRKKRRRTTQLLQLFQGSEIDNKSNKKVEKSLLNNEAYSSSVYTAGVPSNSAVVESNNKFDNQGDSNSVHAGQSIDGIQKRIQDIVRVHYEMEMDVARSSKIYFPDFNLDVVIQRITLYYEKLNEKKQEFDMREMKRTKTNFTLFDRIRQAKNTTIRGSFVDKEND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WV14</accession>
    <name>A0A8S1WV14_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1040196</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WV14"/>
    <dbReference type="OrthoDB" id="285890at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8193633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="52" mass="5699" checksum="4F911724C8DA92FA" modified="2022-10-12" version="1">MKPILAIVSGSTALFAVYFLNGNQPFFDTNSNLKCPVLKDVQSVKNQLSNPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WZE4</accession>
    <name>A0A8S1WZE4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1040172</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CFAP97 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="308210at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029488">
        <property type="entry name" value="Hmw/CFAP97"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13879">
        <property type="entry name" value="Hmw_CFAP97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8193585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="215" mass="25675" checksum="C71EAF41A2E9A02E" modified="2022-10-12" version="1">MTNIWNKNEIITPMSEYNLLYQYNKHVNQVQNIKEQPMNVEKPFHKKKLKCSTSKKLLEIKHPENTENKILVQKILTINNQPSSYALNDDLPIKPSNLGQKILEMRKLIEDNEGIEKRIKQTXIQNLMLDLLKLINNRFCLVLRKIGLCIIIHLLLKNHQKWNFNQEKKDLCFMKNLMSQMDTNHLKQLFEKLLKLEDPIFQIHKENKYQCDMIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XEG0</accession>
    <name>A0A8S1XEG0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Gamma-tubulin complex component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1210047</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000121">
        <property type="protein sequence ID" value="CAD8199191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XEG0"/>
    <dbReference type="OrthoDB" id="5860513at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040457">
        <property type="entry name" value="GCP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF14">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="8">
        <location>
            <begin position="163"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="7">
        <location>
            <begin position="450"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="852"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="978"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="909"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="985"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="996"/>
            <end position="1005"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8199191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1031" mass="122284" checksum="9BF4E25686EE54DE" modified="2022-10-12" version="1">MEFNAQKSVFDLVRLSFPQQTEESTLLNYKTYLYRILNLKTKYQEMDDMIIEKEINDIDNKVLQNRLRECIQKFKKSTIQKNQEIIHFVIRVSQLGKQEILNNSQIKPIFENSNHLKQSIDIENQQTTNQTINKIENSLIKSMIVERQQNSIAQEVNDRDLMKDLLFCLQGIEGQYIQYDASTDSFCLRKDLNVSISVRQIVNLISECGWLFKKISSFCQIQQSNLIVQALQNVIKLELSEYYKLIANLEGLILSSQLITFKRIHFHLQSSYDYMLFINQLLSIIQTNTPLGTTSCLIINVLEQFSKHGCTQTAELFVRLQQASCQPLMKYINEWMFEGNLLDIANEFFIERDDQKVLTKDQGELWRKQYRINYDKVPVVISYDEAYKIYDTGRAINWLRKQCDNKNWYLDIQPLTINMLQTKELSQLINQANKNTNTQLVNLLFDKFKLMDHFKMIKQYFLLGNGNFSQLLIETLYTELSKNAHLVYKHTLTGLVESTLRLSNANQIIQQRLSVKLLEAQKNDIGWDIFCLDYEFEEPLKTIFNHRTMLNYYKIFNYLWRIKRVEHTLIQSWMQQIKNKYHLNAKSNVNKALNLSLQIMNSMIHFIKNFFSYLMLDAIETPWKKFMDQINQIENLDHLIKLHEQLLNEIIDKTFLHQKQEEIQIILLKLFEISFRYKQNLENLFLYVRELVAQDRSDKFEQSLDSIISHKTTPTYNINIDKQFKGQEIIKLLLQLRQMYREQTLELIKHLQNEEKLKFLLFKLDFNEYYYLLSQEKLFSLGFDKFLQQCLPISIKETGSQKVISDQPQPQQQKPLIPQFSQKSMTSIQQDYIQIKEGIRLLEEQKKQKEQQSKILKQSQQKEEQIKPLSKPPLPFPQQQQPMLTMSPINKQSTTSSVSSSNIFQPQPLQNFQQQFEQQIQQQQQINQKLQQLQQQQQQMQQYQQLQLQPQTKQPQLPKFISSSSQMYQVQQSYQPNFNNSVPFSQNQSQSQISILQPNRNDLSLSQDENLQQLMDKQFNVDDDSSDEDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XGH2</accession>
    <name>A0A8S1XGH2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1240076</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000124">
        <property type="protein sequence ID" value="CAD8200350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XGH2"/>
    <dbReference type="OrthoDB" id="285272at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8200350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="228" mass="25833" checksum="BB8EBFDC3C389C24" modified="2022-10-12" version="1">MNQQFFGNTRLAIQRAQQLHLFFTESVIYDQFKYTAKMPQYPKIVFLQSNPFVRNLVEKQFVQYSIVDAQKTTGEDNVARLAEQVQLQSTQTNGALLFGFPNNAIEAEKLDRLLDGVNLAIRFKLSESLAQKIAGSYLSCQSCGKVFNTSLPFVTPTHPGYQNNCQTPSKCALEQSSAPADQINAEVANYYQQKGAYLEYEINEEHLLYDSQEFFEKLDNAVATNIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XIJ6</accession>
    <name>A0A8S1XIJ6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1250026</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000125">
        <property type="protein sequence ID" value="CAD8200578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XIJ6"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="113"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8200578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="174" mass="21445" checksum="ADC74B4304DC4E2A" modified="2022-10-12" version="1">MDSQSLSLFKKKQQKEISQALLYERQIREKELQKSELVLKSCERMSLITDRQIQKLKQSQLSTVKHQQNEHRFKSLESDTIHLRPKIELDQEAYDRWIKYQKRQLRRIETEKLKKFESKIERADQIKMMKQQEQKNKQKLRIETQIQKEKIHDEYTHRLYKLQKKVYAKQIKFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XLT5</accession>
    <name>A0A8S1XLT5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1280105</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000128">
        <property type="protein sequence ID" value="CAD8201618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XLT5"/>
    <dbReference type="OrthoDB" id="287883at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001611">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8201618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="490" mass="58480" checksum="8313BC0165403DEF" modified="2022-10-12" version="1">MDEIKLVESDDELEYQPQTLKTQKQHQPKSVQEMREQFRMQIRKERIEEQLEKKRMWFIPKQRLQSSQMNFSINNLFNELKNVYVQKDMNEISQKLHLIANIIDDRNRIHSLYLTQRNFLQFKDLLNDESYYIDTYTIYLQLTQDDEYFQLPIEDIQWSIERSLDLLIENKQIDDLANLILGYLGNVSSKLQQIQNYLLSKNTIEILCQLMKDSLLHNVCYLIQTLCSNIPEQYKPQLIVILKHLKINKSQEILQTLFIINKQCFYYDKEINYFEIFQLLFNYLNEYLINENDFESFYSALLQMKYLSDFQQLYAIFQQFSILEIINKLINYKYQKIRTAVLYILQNLCKQGHHVIQQIFSQQELIKKLINILTTDKPINQSLVIRSIQHILKYGNQQDFTVLISLGLAVHLKSTIESAEPYIVLQGLECITSLMNYGLSLQTQLNVPNPILAILQQNQLIQAIEDLQYHPNKEIFTQSQKLITEFFRTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XSU9</accession>
    <name>A0A8S1XSU9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1360081</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000136">
        <property type="protein sequence ID" value="CAD8204187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XSU9"/>
    <dbReference type="OrthoDB" id="296677at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CS" evidence="2">
        <location>
            <begin position="68"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="214"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8204187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="245" mass="29186" checksum="F92132136EC9E4C5" modified="2022-10-12" version="1">MIDENQLDCQTIELLITRGFGQQILDLITEEQIILESHLQCAKQSIQNDLLQLEQLKKRFTNHDNHVLIKPIILWAQSRANILIKVKFAHRIDAPAYISVKNHEIKYINEKLSISAESDQNNILTKFYLEIPLLNKIHQNIEWQVESVGTMVLNITKVESQLWLHLSKNKNDKFQIWWDLKEQIGKDMEDFSKLIDNQEDNFKNKKKQQKKNDSQQQSQQQPSSIKKNLFITFFLRLKQWFNKWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XT84</accession>
    <name>A0A8S1XT84_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1360079</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000136">
        <property type="protein sequence ID" value="CAD8204183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XT84"/>
    <dbReference type="OrthoDB" id="297024at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8204183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="140" mass="16722" checksum="5886C3A327556F24" modified="2022-10-12" version="1">MGMCTSQQPKKPKPLIRLIKIKMETCHKSKDIEEAIQIWNKINRVIQQLKSTSKIPDDLREDANKINKFLKSHRNPKFLETFFSYYDQFQYKLNEQINQNQELWIQFESIFSNLDLLNDKFTELGYKSHLGCTQSLTKYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XTH5</accession>
    <name>A0A8S1XTH5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1370038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000137">
        <property type="protein sequence ID" value="CAD8204380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XTH5"/>
    <dbReference type="OrthoDB" id="304248at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="96"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="280"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8204380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="307" mass="36744" checksum="8F6F1DADCC33D3EA" modified="2022-10-12" version="1">MFASTPKIKTYSSLLQNSLQHKPIQQPIFTELTPSTPQKYNILKNVKMLNVSSQPTENSFRSNNSKRIMTQDSPQINKENVNFRIDQSSSQIEIQLKHYMQENKKLSDLVSKLTREKQELLDQITNTNFNVIKQRVERLESVIDHQTNEIEDWKKKYKDVCQNDQSTSFIENIEKSYIKENERLKQINQTYLQKIQNLEQNIKDLEYKVNDQNNQIIAYEEERINLLEQPNNVIKFNLEEYITLFEKNISDLIKIENDNQKQYDNIKEQFQYFHNKMNQMNFKTNNINQLKNILNELKNNLKKQINE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XU65</accession>
    <name>A0A8S1XU65_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1390052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000139">
        <property type="protein sequence ID" value="CAD8204931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XU65"/>
    <dbReference type="OrthoDB" id="296808at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8204931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="207" mass="23775" checksum="0D943C6349F8B6F7" modified="2022-10-12" version="1">MSDSQKIVGIVFSKLESKRSHPTYIVETKAGNLIYRQAVNLPSEQIEKFNLIFSKQIADKLYPSQDKRLFYKGTELDIPDVEFDDENDQEDSKSTKQQCNCQSKQTSKSPNVQSLQQPLQLPSKSTIKLSSNTILTYDKIDEEFKQASRIDCNLIKDIKNWEQAKISKKKQVMLCISNNCFVKLADLRFKNPSLVLDFVLNNCILVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XVB7</accession>
    <name>A0A8S1XVB7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PPENT_87.1.T1390088</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000139">
        <property type="protein sequence ID" value="CAD8205003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XVB7"/>
    <dbReference type="OrthoDB" id="1413014at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005745">
        <property type="term" value="C:m-AAA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1690.20:FF:000012">
        <property type="entry name" value="AAA ATPase domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000019">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000003">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050928">
        <property type="entry name" value="ATP-dep_Zn_Metalloprotease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011546">
        <property type="entry name" value="Pept_M41_FtsH_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655:SF2">
        <property type="entry name" value="AFG3 LIKE MATRIX AAA PEPTIDASE SUBUNIT 2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06480">
        <property type="entry name" value="FtsH_ext"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="174"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="287"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="18">
        <location>
            <begin position="377"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="20"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="42"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="76"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="110"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAD8205003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="803" mass="91996" checksum="00B58073445FDA08" modified="2022-10-12" version="1">MLRLSLTLMRRNVFRFSMPFMPFSTDKEPPKGFQKFQRKSKIQQEQKNQDEKEQKHELEEKKEQNEEVIKEEEEMIKEKQEDKEQQQEIEKPQKKKYQFTYDPKTFNLQDENKKEDNQEKQEKQEQQQDKQDVKEEEIKEEQQQKQQSQKKSPLSNLNYQKLLEYFNNPKNRKYVYMFLGVGGLAALYLYSNQDEEITYTDFLKNYLETSQVNQITIFKNEISRVNQASISTQSGISKRLMIMNVDNFLENLERFQTEKGLSPEQFIPVTFEAQLDKGKLLDRVLNILYFGVTVTLVIVLFQSLKGSMGNINKGSGGAGGNDVFGFGKSNVKQFGFEQNVKVKFKDVAGLDEAKLEIKEFVDFLKKPRKYKEMGAKLPRGALLTGPPGTGKTMVAKACAGEAGVPFFFVSGSDFVEMFVGVGASRVRDLFKQAKAKSPSIIFIDEIDAVGRKRDAKFGGNDERDNTLNQLLVEMDGFGTDTNVIVLAATNRKELLDPALTRPGRFDRSVDISLPDIEGRKQIFMVHLAPLKLDPRKTMEDYARRLATLTPGFSGAEIANLCNEAAIMAARANKIYVDSDDFEMASERVMAGLEKKRTISEEERKIVAYHESGHAVVSWFLQGGHPLLKLTIIPRSKGTLGYAQYLPNESNLESKEELLDRICCILGGRIAEEIFFGQITTGAYDDLKKAYEITHNIVTKFGMSESIGYIGFQEGEFLKSYSDNTNKKIDDEIKQIIEDQTQRTRFLLREKKEFVDNLATRLLEKETLDLQKIIEILGERPFPPRSNYKAYLEIKKEDSKQASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y165</accession>
    <name>A0A8S1Y165_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1400120</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000140">
        <property type="protein sequence ID" value="CAD8205374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y165"/>
    <dbReference type="OrthoDB" id="448466at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00084">
        <property type="entry name" value="HMG-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050342">
        <property type="entry name" value="HMGB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112:SF32">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN DSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="7">
        <location>
            <begin position="13"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="4">
        <location>
            <begin position="13"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="89"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="52"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8205374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="115" mass="13964" checksum="BAACD8CA1FCDFBD7" modified="2022-10-12" version="1">MSLQKHIFQEAPPKKPLTAYFLFLGDERQQIMKNNPGSRISEITQIAARMWSELDQKKKEEYQKRTEELQKEYEVKKKEYEVKFGEIKKKSKKKKHQNNDDDQEYEKKITKKVRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8I4</accession>
    <name>A0A8S1Y8I4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1550040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000155">
        <property type="protein sequence ID" value="CAD8209558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y8I4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8209558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="169" mass="20016" checksum="13220CC900DAA86F" modified="2022-10-12" version="1">MRHQSNGQKIFIEGSHLLLTDDSIHSFKPRFSKYKNFYTKKPLFEYHYVTPTQKHAKEILDKICYVNKKALITERPESSFQKRKDFSVDQCLTTRGSTQQKYIRIKSINRSNIKQIIRLRLVSIYIGLKILQSHLNIFMNMYFLSLDSNILAISIAFYSLLIYLIDQIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YDN4</accession>
    <name>A0A8S1YDN4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trichocyst matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1650102</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000165">
        <property type="protein sequence ID" value="CAD8211900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YDN4"/>
    <dbReference type="OrthoDB" id="297038at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035744147" description="Trichocyst matrix protein" evidence="2">
        <location>
            <begin position="16"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="60"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8211900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="397" mass="46666" checksum="48C5BA77FF9DEE7B" modified="2022-10-12" version="1">MKLYIVFLVICAVVSQNINTELTKDDQTLLDSMFKDKFGMNVLNDIQSEYRKDSEKKDQLVRIESLLNTIERQIAQDEQDDLEQLTKDQELYNQQLESLKEKIAEAQYDYSSIDATIRLLEDESNRYDTQINEKENIVNEYESTLIQLEEIRQHENAYFEITRDDSFAICSLIKRARYLIRELLPRSPRNVAFVQQYTYEDNSHQFSTTDVLQQMKDLQVEAQETLQNNEPFLKIINEFVTVSTQTEAVIDTVRANAIINLLQQLYDQVDSTSRVQLTAEDDRENLHQRIKDQINGELDYLNLQLSILYDEQDGVEAHIIAAQMDQKDGEQRLANLKQSYQDLQKCKEDSKVNYGVRSKERKEQLKLLRKAREVLLNEFDAVRCYIEDILNRNLSQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YEC3</accession>
    <name>A0A8S1YEC3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1680040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000168">
        <property type="protein sequence ID" value="CAD8212435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YEC3"/>
    <dbReference type="OrthoDB" id="286061at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8212435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="133" mass="15808" checksum="22C90FCC97A9D613" modified="2022-10-12" version="1">MQNLHLPTSQLYYEICALSRNKRINDIERRALKKLVILDDKYIFDVIDDYNQKRITETKLQDQLILLSKDVDMKSDTIDECQQEQDYQFNHAEAKKVIDFIEQNPQIPEEIASPLGAQLWKKRRAERQSRKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YFJ3</accession>
    <name>A0A8S1YFJ3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1640066</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000164">
        <property type="protein sequence ID" value="CAD8211607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YFJ3"/>
    <dbReference type="OrthoDB" id="292244at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8211607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="502" mass="59743" checksum="D9C83EBB950E6A9B" modified="2022-10-12" version="1">MLDNSEIIIRESLESYLNYQHVDCVLKVEMSKPHLRQKYFECLQLESFVEFVLTCLELVNTTVLMKQNYQLSHKLHLQYWKIFNELPQPKDEMVSFILIAVWFLIHSTIFKLQTQEERNKIDQRFLFNSYHVLLYYINGFYVSDEFVQQQVDMYLNNRYLDYRLNSFQKKNLKKQQMQQTQTNLFPNVSIPELTQVQGATQFQKELRFRKKQKKQQSEPILVEQPPQQLTRMENRSDRSGRFPNSIKFNLNQLSPSINSLLNLSNKATPRAKLVTHQIISHNFNQAKVSNVLKSLDEPRTTLTQMKESRDNGKGQRLLDKYRVTQPPLEYFKKHPKVDHLFRDMLEMKLVLQQSHQHIYTSPRLAKEDSTALREKLTQYLEERTLKQNLNPQNDILQQSPQFGSNMMITYQHVRTPSDGTRMQETYSVFPQGQISSFLQTQITQSPQPKSNNKFIYEDKRKQYTDRFNKATSNQTTDDKISNLQHNLLVKQHMIQQHLKKQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YLB5</accession>
    <name>A0A8S1YLB5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphatidylcholine transfer protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">START domain-containing protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">StAR-related lipid transfer protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T1670075</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="8">Interacts with ACOT13/THEM2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000167">
        <property type="protein sequence ID" value="CAD8212302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YLB5"/>
    <dbReference type="OrthoDB" id="5403181at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00177">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000017">
        <property type="entry name" value="Phosphatidylcholine transfer protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051213">
        <property type="entry name" value="START_lipid_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308">
        <property type="entry name" value="PHOSPHATIDYLCHOLINE TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308:SF14">
        <property type="entry name" value="START DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00234">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="114"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="START" evidence="13">
        <location>
            <begin position="529"/>
            <end position="712"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00077188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00079049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8212302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="723" mass="84621" checksum="16197F7354233D7A" modified="2022-10-12" version="1">MQALSLQKLVLSAQQMAQKEHFAGSWESLVDMFDKRLQLYKYNRVSLITECKPTIGIEFYPAIKNEKDLLNHIMRLHLYLPQVFFNQAQFYEFSTALDDRNIFDRRNVGSLLGMAFMGIVGFLALGFTIMGMIAGGVLGVMLGRYAGRRMKKTFTSQKVLLEFDIYSIRLRCYIKWAEERGKTYRYNVNFVRFIAEKLLLETKTALHYKQFNADQQKQAKKIILKVAEIFKEKYFIHALILSLKMSHEYLRILRLLSENSELNRGDIEEFQHFKNFRAELEVCLMKAIDNVLRPLVRLLEDSNPKSKVLKKIRDQAKCFLIQKDVIQLTKQYPDPQMRITYIKMLAEKMKQSIKNDSIISYLKKQHFRDIEQIGIVIDQQIDQQQIEQKQIGDIKQIELEIIQEENEQNLTPKQQLKSQYSAGSESEFQPLQEDCLKSSQKLQELQLKITQHPNLVFIDSNQGDQIIQNSLAQSLSQADIKQILEPEQQVSLQHRQSVDINQQIVQYSEETTKKFELFLQFYEESTDSWECVTTKDDIYIYKSMKPGCGSVFLKGHSFIYSHDKQVVFNAVYQKEFRTKWDKIMQKFNIVRHEREDIDIMYYVVVPPIPIVSTREWLQRRVLRYDFPYKGQICLLFYSVDLPEFPITKNPVRAHTEIAGYVFENTSSGTKITFVSNNDIKGSIPKLLVNHASAKGPFGWFGNLRKACDLYRKHNGDLSRIVIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S418</accession>
    <name>A0A8S1S418_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0030247</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S418"/>
    <dbReference type="OrthoDB" id="415459at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000001">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF18">
        <property type="entry name" value="PROTEIN TIS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="19"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="19"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8134322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="212" mass="24150" checksum="E471FC8B710E00DD" modified="2022-10-12" version="1">MSYQFGGSQLQTQSVPNAKYKTMLCRHYLATKQCAIGAKCQFAHGTQEQRQMNDPLPASALSAMASGIEQPVNKPQTSVFQIPCKYHAQNYCKNGSNCQYMHDPDSVQQQNTINQQQMQVISSPLPQTKPEDPMQIVFQHILIEMQQIFPQDEIIQKLKIAFDQTKIGNLINASECIKSIIHAPERTHEELLKYTNLYNQAIQYFNQISQVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S4S3</accession>
    <name>A0A8S1S4S3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="15">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PPENT_87.1.T0030384</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 15">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="15"/>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S4S3"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000197">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000014">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF150">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 14 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="280"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="324"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="765"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="797"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="858"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="898"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="922"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="961"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="16">
        <location>
            <begin position="27"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="17">
        <location>
            <begin position="733"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="12">
        <location>
            <position position="383"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="383"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="383"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="384"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="385"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="385"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="430"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="468"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="490"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="518"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="596"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="597"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="598"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="680"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="686"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="707"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="710"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="711"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="711"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAD8134592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="997" mass="115277" checksum="8C3C2E8F96A48541" modified="2022-10-12" version="1">MNNYFQINEQISQTEKIILPDDTPMKNKPNTITTMKTSWFTFLPYAFIKQITQPANIYFIVIGILQIIPATSYTNGVPTIYGPLAMMMSISILKDAIEEYSRYKSDQEENNRKSHKYYENQFIECNWKDIYQADIILVMENESIPCDILILSSNYQGGIAYVETKQIDGETNLKQKYSIYDLQQRYHIDNAINFKEKNNNVKFVYDQKNPILYKLSGTYQISNSQVDSLNYGNFIERGCILKNTEWIIGLCIYSGMNCKIMLNCSQERTKRSRLEQYMRICIISISISLALFCLMSSIYEFIWTNNHLNYSYLGLTKDNVFIDLGLWFSLLSYFIPISLVVNVELVKFGQAKMLESDKVMPNSKSHQSNLIEQLGEINIVFTDKTGTLTKNSMQFRELFAQNEKEALLCLSLCHSVVKSKNKLIGSSPDELAILEYCEQKGYLFQGIDNNNHLTINKTLFHRVMNFDFSSDKKRMTIVVKTGNEYVLFCKGADEVLLELIGECQHQQIIHQFAIQGYRTLMLGRKTFSQSEMEIYLTEYKKAQEDKDEEMINKLQKKLEYNLEFLGITAVEDLLADDVGQTIQDLKTADIKVWVLTGDKVETAISIGYSCNLIDSKDMLQVVTQITVGSIEEVFLQYCNESANIALIISGQALTVMLDNPQLKSMLLKRAFYAKVVIVARVSPKQKQQVVTLVKENLPKAYTLAIGDGANDVSMINEAHVGVGIQGVEGTQAARAADFAISEFKHLKRLMFYYGVECSRRNALTILYIFFKNQVFITPYFWFGFLNGFSGQYLYDQWLSSLFNTVFAALPQVLYALFDEMHPSSEYLQWSKTSHNLLEEKPDVYVQFRQQPIFTIIKFWLWVFNGMVQGLIIFLVCTFSTENQNMDNGMTMEFFEDGVFIYGIVIILVNLKIFTFTYTNYNFTIFFIVLSILVYWLILIMVNDHELSPSFNFYRYLVSPQLYLALALTCMLFLFDLAIERFYDFQIYIRKPESIELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S554</accession>
    <name>A0A8S1S554_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA/rRNA methyltransferase SpoU type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0030281</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="241340at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016423">
        <property type="term" value="F:tRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18091">
        <property type="entry name" value="SpoU-like_TRM3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045330">
        <property type="entry name" value="TRM3/TARBP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044748">
        <property type="entry name" value="Trm3/TARBP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12029:SF11">
        <property type="entry name" value="METHYLTRANSFERASE TARBP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12029">
        <property type="entry name" value="RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="tRNA/rRNA methyltransferase SpoU type" evidence="3">
        <location>
            <begin position="1247"/>
            <end position="1388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8134390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1396" mass="165409" checksum="452836944C266735" modified="2022-10-12" version="1">MFVKELLQIQEYVEQKKSSGKQVSLKPLIDICVHKYQKEEVQEALNQALTNELKSEWISQQNIDILILCYKRLRCDVSMEIIEKTFEISIDNLQTTKRILSICTEEQLRNLCSKYISTQGYVNKIVKPIYESLMNVNDQTMIIVDKVKKLYKNLFSLLKVQKQWVLERAMEFVLFSEDIYTEKSQKFVAQQFQEIRSIIYAFIINDDEIRLNFVKYLPQIFQQHNPKNEDFKLHCSELIKIALFSKSVKQLITYEQFWQALHDSYQVYSDLTNNKICVAILNEVLLHIDDEKIQQHFKLFTDLFITLDSFGTHLTKDVWVKMDPFTQKQQFKIDNELKRQLFQSIKPLFILINKAAQHSNEKTIKNCIKSLFKKQIDVNYLKPLIIEIIIPVLNKPVFYLDTPLEGDSKFYELLKQFLVKIPYIDDIIIQMVQHIQYPLAVGAFSVSLKDIKVEITQFSLLIQFIEQNLMRLNLRKRIQLLQAVLNLYKQNTSPEQLFQLIKVIPFDCLFLNNIGELLKSLINEDFLLYAQQQQQKFDINSNYHQFAVIYHLTQDHKLFMNFYEPIISQLQQIYTSTYTQKETKHKILEQLTYLLKTSNQVQFLEQISSGLFAYLTLDLEGAMIVTEELWISSLQHFELVPQLTQQMSKFVGFLFQQQFDNFNIIHLELLIKTLKATNISLQQAKRNKTGLFVEELFLKYGLQLLNKLQTIEQDRQLKNLTLHYLSHILTLFREVNHKPQEYHTQVQNLINNIIDITDQFNAYDSIILYQLLRSVYGPEIAFNKEQSEQFKKVAINSFQQMMYIFGTHTNLDEILAYIDFIIDPCYFEGELYEAVEEVIRVIMKQHQKQWVIQRKIANNLVICLHKYPNRLPTFYYILFKLIKGSELRGYDSSEILQSSEQYVPLANNVKHLISCKGEFYGAYPRFLIFNFLFELGKQGGYEKELLYYIKFLIEVSIKDKYKNAYMQYTKQFRMKLHVWQNICCLKYLLKPENYQKVFGDEKKYLIELEQLIWKAFDTLNNPQYRQYIELMMSFLINNYPDIWAPRLLQTFSDPKLKVNIQIPTTFISCIFLLHNQQTKFSQKFFDKLFPLVSSNVAYIRSLCQTTCLQYIKKQKLEYKDFIEYFESNRECQKIIKNISALIERYEKLMLSFDLDGILTIQFDTFGEIIHPSIIEILKNTCTQSIAELKSEDNMSENPPPPWFQYVERQQLSTQYSQQLNFQRKINLIMDQFDLFKAYQPRQIVGDIIVVATLLEKIPNFGHLTRTCEIFGCRELVLPNKKILQDEQYQAVSVSAEQHLPILEIKEEHLMNYLQLKRLQGYQLVALEQTSQSKSIVNFKFDKKTVLVLGKEKTGLPIEYIELMDNCVEIPQYGVIRSLNVHISAVICVWQYIQQWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S6U2</accession>
    <name>A0A8S1S6U2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GB1/RHD3-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0040117</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S6U2"/>
    <dbReference type="OrthoDB" id="2135133at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030386">
        <property type="entry name" value="G_GB1_RHD3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003191">
        <property type="entry name" value="Guanylate-bd/ATL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015894">
        <property type="entry name" value="Guanylate-bd_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10751">
        <property type="entry name" value="GUANYLATE BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02263">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02841">
        <property type="entry name" value="GBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51715">
        <property type="entry name" value="G_GB1_RHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GB1/RHD3-type G" evidence="6">
        <location>
            <begin position="36"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="544"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="374"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="550"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="565"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8135227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="599" mass="70005" checksum="F8D1552B8EF36C1D" modified="2022-10-12" version="1">MKSTQKEKPIQVVKYTKETQKFEITQEAREFFQQLDGDIGFVVVCGKYRTGKSFLLNKLLDAGGEGFKVDAATDSCTQGIWMYTKPIINPKTNVQIYFLDTEGSESIERSANHDAKIFALAILMSSIFVFNQKGCIDETSISQLQLTTTLAKNIQVQDQNKESVNESQIKYFTPKFVWILRDYVLEMKDKQQRDLTPKEYMEYCLTDESQYMKQNEQSKKIRKSLLNFFKERQCFPLITPIDDTQLSKVNQLSLNQLRPEFQKTLLQIKEYLLNNCSPKQIHSENVNGRMFCAMLDNYISVINRGGVPNIDTAWDSILKNECQQGFELARKNYQNSFNSFFVQNKNNRKLEETFLILSQIRSECFDTFHLIAGVREMNKHYEDYKQKLIDLMNNTETEILNQNDENHQLKNQEIIAQETKKLQDDVEKENYTADDLHFFSADFVSFLLNYDKQASGARKASTLYEYLKLTHSNLLKKLEKSIRLKQNKKGGTQTNINQELAQEIRDYDSQFKLLEQNISQQDTQLKKLEIEKQKLDEEKKKLKDELEKMQNSNKQQGSNQNNIAEKQKLEDDLKKQEKDKQELNLRLKNLTKKKKGCCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S886</accession>
    <name>A0A8S1S886_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0040390</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S886"/>
    <dbReference type="OrthoDB" id="10251371at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15845">
        <property type="entry name" value="SNARE_syntaxin16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000081">
        <property type="entry name" value="Syntaxin 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF83">
        <property type="entry name" value="SYNTAXIN-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="196"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="11">
        <location>
            <begin position="121"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8135727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="218" mass="25717" checksum="4DFFDE2BE92380C1" modified="2022-10-12" version="1">MEETRDTIQKVEILNQEIERLKTSKDVGPKAQFNEQVIQGKLDQALQMIRDIEENLQIMETDDCENDLEYKIKQNAKMCLGISLKEVTPQLRNYQNQMMMQVQLERPSQNLKVDFEQEYFETLILDRNDRIKQLGEKLKKMNELFKEMNRLVIEQGTLLDRIDFNIDQTFTRIKKGKEQLVQANTKQETSDRAQKCICVLVGLNIFIAFLFVIKHTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SBQ6</accession>
    <name>A0A8S1SBQ6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0050442</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SBQ6"/>
    <dbReference type="OrthoDB" id="292964at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF733">
        <property type="entry name" value="RE52890P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP" evidence="6">
        <location>
            <begin position="27"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8136980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="326" mass="38572" checksum="90663F98ED687022" modified="2022-10-12" version="1">MGVTQSSQNDHLEKYLGQDFPDGEILIGFENPSNICYSNVILQALYYCKDFRNRILQHQSSINQNNLLDLTNLLFQSISNHKQKTGVISTKKMMNYIKTKNKIFDGKYHQDSHEFYMWFINECEELLKDKQNNWIRQIFQGQQLTQTECLNCHTISQREEMYCDLSLDLFPNYSLNTCLQQMSKEEQLNGQNQFFCDKCQSKQDAYKRLLLNTLPNVLVIHLKRFKYDERCGQMIKVSTKIPFSQQIRIKAQKQTKTYELTTIIIHLGQGIQYGHYICITKIQGKWFKFDDDKISLFVDQDLHYVYGRSYPTQAQTCAYMLFYNAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SET1</accession>
    <name>A0A8S1SET1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0060174</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SET1"/>
    <dbReference type="OrthoDB" id="299475at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8137602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="183" mass="21150" checksum="EB56E3D2AAF1A6FA" modified="2022-10-12" version="1">MGSQPCCCKNPQDPIQEMKLKMEHRSINHQILVADLKKPIPLEQILPLQEEDDFALFLVMEQYDRLITKSQRKSKSYSPSVDIQIISKENLTSYYLKSKNQQKPSKKTRSLTTKLSTSFQSIGIKSILKKKELINNKSSYFQQVSDVQKSIKSVHFDVALSPKNSINRKADVLKKKIFLRSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SGG2</accession>
    <name>A0A8S1SGG2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0060084</name>
        <name evidence="3" type="ORF">PPENT_87.1.T0150384</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SGG2"/>
    <dbReference type="OrthoDB" id="289975at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8137424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8146515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="55" mass="6443" checksum="550B719AC6EEB2EF" modified="2022-10-12" version="1">MNNEDEMEKQARLMAEKYKDGPKKKGALVKKTQERTQFDSATHEMQKQAQQHPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SN90</accession>
    <name>A0A8S1SN90_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0080357</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SN90"/>
    <dbReference type="OrthoDB" id="294895at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="47"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="105"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8139912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="545" mass="64657" checksum="D4846918302732EC" modified="2022-10-12" version="1">MFPESCKNRSIGSINQSCHDSHRPRQHLINSFGFLSKHESRNSYYDKEDQQDQLLKIKQELNQLRIENTQLKSQNIYYQKDLESFHQQLSDDEIQLKTVRNNQKFKHLLKLLQTKEIEIETLKKKLKMDEITIYQKKVSELQNIIELQKEKLLGNQRNILGPQFAILDELEQDNIKLVEIVKVLEEQLKDYDQLKKHIVTQQVKIQQQKQLINELLKEIRQYKERDLLFAVKHNQKSKQDQQIKKLLSDIHKLSQKRQEDEAYIEQLLDRHRQEIKKYDRRIMEKDDEMQLLQERCESLSKALQEEQIQKAKLKKNEKSSSIVKPSMFSQHQIVGRGSGDFSLITSQRSLSGIGKSKYSKINKQDLCQIVKIIKFSMIINKITFDELDIYLFKNQEETTLDALKDSLQQQIFNLNKEQACTLANYLFDQEDEINIDKPQNNSRIRSILKTLLDNFRLPNNTVFDEIKTNLNANNLEMICNQLQSKFQSPRKKLNVNDLFDLFKENDIKLSKASLEYIESYFFQLNQQLIVFEIDQLKQLIDSISN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SQI5</accession>
    <name>A0A8S1SQI5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0100245</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SQI5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8141666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="137" mass="16932" checksum="22CF962D6C14FBAB" modified="2022-10-12" version="1">MKNLRQQENPIDIVKLWEQIKMLILLSENYQRETRQQRLIHYKNQIDKELYKNEYQIYFYFVYVATYKLIVIEIRYIKKQQYLIFLSNFLVIIRIPKMKTPEIAKEIIKNICYPSIKQIVKKQKHKSIITISMMRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SUA7</accession>
    <name>A0A8S1SUA7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0120199</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SUA7"/>
    <dbReference type="OrthoDB" id="305194at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="86"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="335"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="398"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="529"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8143378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="602" mass="72591" checksum="896C56C8EA26D7A4" modified="2022-10-12" version="1">MYRIGDQMSKTSQSRENSNSNLEECMHNIQSNLKMIYGKDEEEVHLGSYNVNAVSGFHSQFLSNKDDQYDSQRMHYTSQQSNDFMLEDKNKQIRQLNKELADLRKGQQMTQFEQYEHYQKIIDSLKKENVLLQQRVLNNEAVQQLNSDRVIEQQEQIKEYEQKLDQQNQQISSLNLQLKDTKDQLKLYTTKSQISKQEQQSQSKQYNQLQQEYDELQRRYIKQQQENENMKVLIEQLQNDLDKRLETQNDTQVIEIIEEVLQTISQFVREYDYELQKSEYQYKFSNELKDIIKGFITQRDLSIWQNIDRLSSLIISLFEQLTFDVKQIHSDNIKIKNMTLQIESLEQMTVQLEQTISQYEIISNQQQNYLKFNDDQNKQEKEELVNVLINRTLNDQQINCYDKMIKNLEEVNQAQQNEIKELKDKLSFQIEQNQLYQKQYQEEQHQQKIYESKFKKSHYDSLFLKEIMNKLVILIGNEMTTKTIEQIQEIQQKINDQYCDEIDIKSKLEIKEQDLKIQINENKLNMNEMIEKRREIEILRNTLRNCQKQIEMNKEKVDMLVRDLDHNVMQEKSQQFSISYLPKTFKKENENLAQKLQKTMRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SYW5</accession>
    <name>A0A8S1SYW5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0130243</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SYW5"/>
    <dbReference type="OrthoDB" id="283202at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051291">
        <property type="entry name" value="CIMAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580:SF28">
        <property type="entry name" value="BOREALIN N-TERMINAL DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580">
        <property type="entry name" value="SHIPPO-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8144466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="225" mass="25255" checksum="4D8EF35644242C76" modified="2022-10-12" version="1">MINNENNSQHLKSKPLFSVMKLNNNNTFRGQFSEPNQLSSSILQQTNPQLSFPRAERFQSHHAPQLPSPKYLCLTDTKTKRATSFGFGNKQLRPLDLDKRDQLNPAPGTYDVKQTSCRSCSFGMKLQSIRSFDVPGPGSYDLKLLNKTKAFSIYGKINSDIKLPKAASPTTYLPTDKIIQNNRFKQITFGIGDRPLPGLKNDSPGPGTYALKSVFEIKTKKKTQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SZN6</accession>
    <name>A0A8S1SZN6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sperm-tail PG-rich repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0150348</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SZN6"/>
    <dbReference type="OrthoDB" id="286690at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051291">
        <property type="entry name" value="CIMAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580">
        <property type="entry name" value="SHIPPO-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580:SF60">
        <property type="entry name" value="SPERM-TAIL PG-RICH REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="422"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="327"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8146445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="627" mass="70387" checksum="244E9EB64B2A2902" modified="2022-10-12" version="1">MIHGPNSPFSGAFTIGKRYESAIYQTNPYPGPNHYSINPIDKPIGFRFSKSNRKPLYQPSKAPDPGAYDLKYQTISQNAPNAIFATAKDHQQRGLEIGPGSYNWTDQKKAPAFTFQSKFDSIGNQILQNPGPGTYDIKHYHTHQPQNKGLTTSKRVNHLTQSTPGPGQYDVEIPSFSTNIKFPKSQRSFELSQIGPGPGAFDLSLPKQQGITFGVKGNQHIEKSNVPGPGSYKLKSFVEYDERDLKQNKIKGVKIGTSQRSSQNLLKFGPSPLDYDVQKYKHPTRHASFGSAVRQSIIPNQQTPGPGQYMVDSKLSRNGPVISKASKDFSSLDNTPGPGKYNPNISISSNKGPSYHIAGKYDKPQEKSLVGPGRYNISRNINDGPKYTFPTLEKNHIAMRFSKLLGIYLHITYIIEQNQNGLIYYKIMKIIVLIFLLGAFASNIRHKKERHIDSQTLSHQQQNTKELLQVTDIDNESQTLVEETLNQQQNLEEPKPLYNNDGRSPDMGMNQIDNNDAPEAPMMADNVLDYSAQEEVIKGVSLIQVGEIDALADIDTSMPLLDEDFKYDEQALQETPLNIDIIEEVPQQQDQEINQIDDQLQQEVQVEDQQDQLINEVQLLQIGGFYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T1Y4</accession>
    <name>A0A8S1T1Y4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0140262</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T1Y4"/>
    <dbReference type="OrthoDB" id="286864at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021258">
        <property type="entry name" value="Epiplasmin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10992">
        <property type="entry name" value="Epiplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8145476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="243" mass="27351" checksum="907E0E338B20D931" modified="2022-10-12" version="1">MPIYPTAPLTSSVPANLNYSTPYTYAQPLTYAQPVQYVPQPVQYVQQPVMTQSVVAQPVVTQPVIAAPQQPTIKGESRVEYREFQRPVVEMETETIQVQVPKTKYVTDYYPVEYQTEYIPRTVYEQQTEYVPVTKTVPRVEYDAVEREVQRVQYQPVQTVPVQTVAVQPVVQSVVQPVQPLTYSIARPVAQAPVYTQPVIAPALTYSGVRHAYAPSYPGVPTYGQLPQTVRSQPQPNPSNKPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T2I0</accession>
    <name>A0A8S1T2I0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0140385</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T2I0"/>
    <dbReference type="OrthoDB" id="308943at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8145706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="102" mass="12360" checksum="33D2195D10EB5E3F" modified="2022-10-12" version="1">MSNEMNSLSKQFTKTKHITLNEGNDSYELFHQQFIRQKQLIVNKHCNYVNLIRKTQKGEFKVEQSIQFNTQRLYGQMSDDGEYLITWDYQSNEIQIRKYKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T308</accession>
    <name>A0A8S1T308_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0150028</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8145827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T308"/>
    <dbReference type="OrthoDB" id="312599at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="32"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="305"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="305"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="335"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8145827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="587" mass="67076" checksum="48B34E851F96C7D9" modified="2022-10-12" version="1">MYLAQKQMYSQHPSYTNGSSQNVKKMIENFSYGLHQQIGKGFSSKVYKGQNEVTGETVAIKVIDKALLKTPLHHALLQSEIEALSTLDSQYIMKLYKVFQTQNNTYLITEFCENGDLGSKLTKIGKFPEQQAQNVIFGLVKAYKLLKQNGIIHRDIKPANVLLSSCGTPKLADFGFATTPNSPPLLPNVNVGSPLYMSPQALKNNKYSDKSDIWAIGVSAFEIIFGQVPWQATSEKELAQKMVSVQLTFPSSTKVSQECKEFIKRCLVVNEQERASIDELEKHPWIRGPELAFQVNKQPALMKQKTLEQPKEGKENSAKKLPGDSIHRSGKKHTTQITKPLGQQSKQTETSSFEIKSNTSNIEYTIMSQINFCKYLFRVANLIESTNLFRTDLLRDKLLFFAIKNIMIKMYKLKDIDSNSLDIQGFDAYKASNQFQSTQITIQQNFTKYQSYFKNIWSSLQQNKNELSIDKKFDAVFDENFTEFESFYIIFNSVLRQAIKELQKEIDKKLGSDDLSKSLPIDMEKDVVLLDYLVTYFQLSQLTLETFNKPYEFAQKSKIEHIAEGSPVRLTYGHFIEIKNKMSDLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T383</accession>
    <name>A0A8S1T383_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0150219</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T383"/>
    <dbReference type="OrthoDB" id="298145at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8146188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="475" mass="56268" checksum="4753A38D8859F218" modified="2022-10-12" version="1">MKRIAHRRREPTQTTTCFSDHKTEQDEPKQKDDRLKNWNIMFGNFLGEGLVRQKKRNKHIDPAFLREAPIFCGNNVYNSPSVRDNFEKFIKGEISACEFLTRKNQSELLDRQRPNSPQKFKLFRVEQDNNHGSDHPKQSGFLKLLKDKSDEISKKSRIQSRFAVLRNLIEHKIKLKEEEDIIKRSRELKNVGVIKQMYDISDEYIKLISRRYIEKKITPTIQKNIINGNQIKLIQHQNPYQNQKTAIYENETKNKYDPPTQISAIKLQINQKAKEYIDELQTRDFIQNEKAKKKIFQNSQYKNIVHQQSQSLHSLRNVKQIVPFIKQNYLKNQYINNDLELIKYITDFHVEDPFHDKEIQSKVRKSLLISAGIVPHNQIQKIHQKVYKQSYLNPKDDISQKSSRTINDSLNSMYEFNVDSLYNQSITLQNKLLEKQSEGFSKTIRSIQKNESLKKEIIQRNVQKLEGLKKLKIDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T548</accession>
    <name>A0A8S1T548_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0170005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T548"/>
    <dbReference type="OrthoDB" id="291627at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8147520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="284" mass="32489" checksum="4D4697C8FEB85DCF" modified="2022-10-12" version="1">MLNTSSGQRIRYVKEASGAKSTTQSFKYKSKECDLSANDSTSSANLKPLIQVKQSYNQHFATALPLRKLASPQKQAPVDGKIVERTYSSVRTSNYVKRSSVAPEKRVENRSVIYEKRSIKMPICSPSSTNVIEEKLEDDQIQGQGSEIQSQKRRKSITFFELTANTNQQQDNQEIIEMMSKYSEKELKQVQNIRVIINGQFDEREIYVDISSIPFEWKLEQIAQQLNEIYKSQFNQSLRNPAISVLIGKIQTKKLSKEMKKYELVIMSLKGQVKESLIVLEDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T5U4</accession>
    <name>A0A8S1T5U4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0160237</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T5U4"/>
    <dbReference type="OrthoDB" id="306246at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="205"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="208"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8147158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="574" mass="68593" checksum="22EBD12B273BA5FA" modified="2022-10-12" version="1">MYFRERRALSNQKQYQGYEDTKIQLDLIDKFINPSYENPISQQPSQKSGIRQLNQMDKSYQIPKVVVFPEEKLNEEVAQMQQALDKQSQTLKLLLKEQKEINRTQVLKSKLDYLKTQVQTLHLKLPQLPQYEQFYEIKHEINSLRYSFFNKVHQPQQPFIQPPQIIYQQLPQPFSQQYFQQAYLPFNQQQSHFGSQRDYHPFQHLINPYQNPYNPKSQRVKSNTKDKYSNSKGKAQKKTQTKNQNSVSKAFHSERSSRQSIPLISKGLNQKTKQSFFMDNAGFQVQQKIQKYKLKNIFNFVRYAMRWKIYCRPNKMLWRKLYRHSVECKAILLKTSYPVALKRIRDWCKMVLAKVENYLSKIKEIDFINPEKILTDKEIDQSFMQLTISTKYLITSLLTYCTSDFIIPELKFLSYLQFFDEPNIDRGLFISKRVLFWKENQLDMTKTQQQMIIGDLVIIVHILPALMDISGSYFLIKCMVSLIQIHFMKQFDLQIINPNPSYRLIQLNVKEYVNGKLSLKLQKVENFDDDRYIIGVYEEQQFQQFFQKRPYFQDDMQNTLTEIHTNLLQALISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T8F5</accession>
    <name>A0A8S1T8F5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0170078</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T8F5"/>
    <dbReference type="OrthoDB" id="287987at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8147666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="173" mass="20236" checksum="6F1CDC1F46198432" modified="2022-10-12" version="1">MQVKHYRLISELYSPQNKSSSSENIFRVTKISSSQPKLSSLFPSRATLSTGQSFTTNESAMKISAIIPKMHDERPYSRVTTGFQKNRTYLDCKRLTSQTKYMQTLDDWEMNTIEEIQMRRTSNKQTINCKQLCDNNIVNKMHFTSQKKVKKNSMSKVIIINHQGKPKIYYYMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T9E0</accession>
    <name>A0A8S1T9E0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0200380</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T9E0"/>
    <dbReference type="OrthoDB" id="74764at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="129"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="438"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="438"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="455"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="485"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8150631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="527" mass="60454" checksum="24AEF75725796EE8" modified="2022-10-12" version="1">MHQKLWIPIPNDFNPKQEHVIIQGEFTKPNGKTRLLYGYDRFVVCVKKDGQFKKCIKLEFDTKFEILRQAIQKKDEDDDSLGPIIGIQFQRDNAGIMPTYKLSASSKLILEWRQYLSPRINQWQFHHMFRVFKKIGKGNFATVYMAERIEDGEEMAIKAFAKQAAYAEENGKEAILNEIAIMRKLHNKHLMRLFEVYETSNSLYVALELLEGGSLYDLIKDKVPINTKQIQQIMVGILIGLQEMHKKEIMHRDLKLENILFKIPKKMETVVIADFGLATYVNEPVYLYCRCGTPGYVAPEVINIKDMKSKYSSICDIYSLGLVFYLLLTGKPAFNGKSYATVVKQNREASIDFDIKQLQNAPGAAVDLLKQMLEKDPKKRINAEKCLHHPFLSDITKIMIEAEKEDSQNIDEIDEGNEIHNMMNKINDEYSKFDATRNINSPLQSPKQSPGLLMQKQIQQQKQIDSTKAVGHDSPLLKGKVDSIDSAQSIGTPKKNNQQYQPSPQIKPSRFAKQAENNPLLKYAKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TCB2</accession>
    <name>A0A8S1TCB2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0180161</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TCB2"/>
    <dbReference type="OrthoDB" id="5590282at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20507">
        <property type="entry name" value="CYCLIN_CCNB1-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000001">
        <property type="entry name" value="G2/mitotic-specific cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046965">
        <property type="entry name" value="Cyclin_A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001771">
        <property type="entry name" value="Cyclin_A_B_D_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 4" id="KW-0195">Cyclin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="5">
        <location>
            <begin position="119"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="6">
        <location>
            <begin position="212"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="5">
        <location>
            <begin position="216"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8148629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="336" mass="40141" checksum="1D69B0A50F070A3E" modified="2022-10-12" version="1">MIHDQYRFFGKELVNSSGEFQKENTDRNKPKHLTVLPRYLKLNLWQEERENKMEVENNTHQLCSFDQQMIKDPQYISLYNQEIFTYLLTQEEKYLVSNNYMNEQQQPDLNARMRAILLDWLIDVHLKFKLRDETLYVTTYLIDRFLNLKTTTRQQLQLVGVASLFIACKYEEIYPPDLKDFVYITDNAYTKQDVLDMEGQILQTLDFSITQPSSYCFLQRFGRIAGLDTKNLCLAQYLLELSMIDMKFMNYKPSFLSAAAIYLVHKIRKTTQSWNEEMQKMTGYNEQELRYCAKEMCLVLQSQEKSNLQAVRKKFAQPKYLEVSRIRVERQVKQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TCU0</accession>
    <name>A0A8S1TCU0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">60S ribosomal protein L27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0190241</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TCU0"/>
    <dbReference type="OrthoDB" id="2365484at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06090">
        <property type="entry name" value="KOW_RPL27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.770:FF:000007">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001141">
        <property type="entry name" value="Ribosomal_eL27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041991">
        <property type="entry name" value="Ribosomal_eL27_KOW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10497">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01777">
        <property type="entry name" value="Ribosomal_L27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8149698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="152" mass="17875" checksum="0341B0287049DAE7" modified="2022-10-12" version="1">MGKFLKPGRLVVMLAGRYAGKKAILIKANEESTKDAKFPNGLVVGIQRYPRKVTKRMGLKQIRKRTTLKVFIKQLNLNHIMPTRYRLEESTLKEVRDRIERVKESELKNVEKRKELRKNLRKYLAEKYRTLPAGSLADKKAQSRFLFSKLRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TCV0</accession>
    <name>A0A8S1TCV0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0180418</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8149106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TCV0"/>
    <dbReference type="OrthoDB" id="303865at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22584">
        <property type="entry name" value="Rcat_RBR_unk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000894">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="102"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="305"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8149106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="444" mass="53070" checksum="87AB33B70C01F7C2" modified="2022-10-12" version="1">MDDYLDDQYYTELEEDYDNNNVNNNVTLLSANNLYQEFQDRLEIIKDKIGETSQVVLNLLIFFNFEVKTIYDKLLLNDQSEQIKIQLQEQGIYKVSQLNFHSNMICSICEDNNTQGYSLNCNHKFCNNCWNQMIEVQFSSQIPLVRCLQDQCFERLPNSFLEQYSKYTQILIKRFMQHDDEITWCPGLNCENVFKCKNFSHSIKCPCGIKFCSKCRQEKHYPIPCDILKKVLEYQQSNDYWVTLHASKCPQCGRLIQRTEGCFHLKCLCGQHFCFKCSGPWAKDHEKNFYTCPYMNTNKNPSKYALSLKNELQSINNNIRIIEQSIKELKQKVKKVQLDNLLEYSDKIIQLLKKSKLFLYYKYYLMENKDVQIYEQTFQQFQKELNIILIIIQNKILEVEDNEQIRDKMILEFINFEDSEKKVLRNLKIKKQFIKQYIIETLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TDR1</accession>
    <name>A0A8S1TDR1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0190068</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TDR1"/>
    <dbReference type="OrthoDB" id="293697at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19756">
        <property type="entry name" value="Bbox2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B box-type" evidence="2">
        <location>
            <begin position="13"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="68"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8149356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="397" mass="45759" checksum="C99C58D0BAFC15A3" modified="2022-10-12" version="1">MSEDIKSRVMNYLNCPYGDMHEGEQLNFVCLETTCKEMGLICPVCRTQKHSKHKVIPLKIFLADIYTNVNAKQNQNSLESLLEQLDQVRLKMLSSLKDIVQKMVLQIKLLEDQINLSHKNTKQRLLEQNQTQMNFPQLFQQILNTQYKNVDLLKQDVRKIIENVSQQQPGKIEIDFKPQKLFDSYSKASQEAINQLQMLLTSFKQSTQKISKPIEKFTLPIINHNQINFIFSQTMKSTTINVQDFKIATQSVQQNIENRFILIEPSIQDSCKIAFKILHLTNFIGLGIALKNVLQGKNLRFDYQAMGHGSYMASSNDHVWSHNKKEENMISKSFPFGTGDIIIIDVNLENKIVKFKNKNSLNEPISLAFDIPENDEIVFCVNLGSAGERVEILDDWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TEM9</accession>
    <name>A0A8S1TEM9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0200205</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TEM9"/>
    <dbReference type="OrthoDB" id="277802at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12246">
        <property type="entry name" value="RRM1_U1A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000039">
        <property type="entry name" value="U1 small nuclear ribonucleoprotein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="48"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8150328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="213" mass="25009" checksum="B68D88074FC7E61A" modified="2022-10-12" version="1">MLQNLNVPPLFSINREEILKPVQQAQVEQVPTKQTVEIKEIDLNIPNKTLYINRLNEKVTAEEMRQTLYALFSQSGKVLDIIVKKNILMRGQAFILMEDEDSAIKAQKAFHNTQLYDKVMKVNFAKEKSYYISKGDYEEKEKLPMSDKIKEHKKRIQDKRQREMINQKQQREAPTISMPLTMNQVYTGTSNKIKVLLYFQFFDSKLTKQSLIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TFI4</accession>
    <name>A0A8S1TFI4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0200008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8149936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TFI4"/>
    <dbReference type="OrthoDB" id="10264588at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="87"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="132"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8149936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="357" mass="42656" checksum="884D739CA235780D" modified="2022-10-12" version="1">MFYIYLYIVYNNNRQSDLIQKKKDEQQILLSEEDDIILKKIDFSVKQGGNCVSIAFNSSYSIMVSTHKSIINVWNFNNGKLELLKSLNQHNDWVNALVYSKKQNSFISCSDDKTIRIWKDQGENDWISSFAYEQHTDRVYCMTINQNEDQLFSGCFDKKIIIWKLDFDKNELIYQYSLKKYNHRIQSLSLNQSESQLVSCDDVQLIIWQRGLDNKMQFKYFVKPSKGISGQQVKFIKENQFVWVPNDEQVNEIFIFELQEGVYLENQEKKLQMNENKKLQNMNLFPIYYNKQRNLMIIRHKACIYFIREQKNGKFKIVEQLNLDTNTINGAATNNGLHFVFWDDQKREYSIYELQYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TJN9</accession>
    <name>A0A8S1TJN9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0240133</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TJN9"/>
    <dbReference type="OrthoDB" id="300340at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="129"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8153295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="499" mass="58592" checksum="B9EE897657BC50E0" modified="2022-10-12" version="1">MRQKSDSCFSSHTNRLDTLWKKGYPQIQYGSQLVTLNGVKIKDGQKNILQFKFAYLQNKYLVVDQKFVNLENIQMKKVILQQNQQQSMAFLKFNNNFDSFEIIGSVHLVNEIYNYCKLYTIQDNFENYYQIMQLMGRGSFAKVFKIKQIENQKIYAVKIFNKMKMKENEEENQKSLWKEVHIMRQMNHKHIIKLREVYEDNYKVYVVIDLLNGGDLISHIEKQIKVYDESLVRKLVYNLLDALVYIHERKIIHRDIKPENLILKEENDISNIVLADFGLADYYQKDAQYLFKRCGSLGYVAPEILQDKHYDFKVDIYSLGIVMFLLLTGEQAFKGSSTQEVLQNNTYGKIDDYKLESCHVSQDAKNLCRQMLNINSNQRLTAEAAIKHPWFKVDNNNISLHAISINRIPQNLKGQKEFLFSNTPLWVNKSLRSIGDSSENLAAITINVRDKTIQEILDNHNFKLDSTVYDLEDDYVDEQESISIRIENHKLLVKQRSLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TK27</accession>
    <name>A0A8S1TK27_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0220366</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8152273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TK27"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="132"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8152273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="476" mass="56486" checksum="A39575139D51DCD1" modified="2022-10-12" version="1">MYEDLNPSCNYKHCRSFFQISFQEMKWNKSNLPFLPTIFNIEDDVVCISKKQQIKRRTYKLLDQYIYYKNKNEKIVWINYENAIIELISDQELGIGVRLTKCFDKVEFFGNANNWYKFMKRFAVQYDFNRYYTLIKKLNKIKFGESFKGKNKLDNSEYIIKVYQKSQIYDDEDRQSFFKEISVIRQLSTDLTIKFYDLFESPDQIFVIVENLPPYTFQEYVQKNPFFSEEKAAKAIFRIIKIVAYLHSKKIMHRDIKLENFIFRQLDNLDSLCLTDFKLAEFYDDEGNYQFKRCGTVGYVAPEVLADQNYDLKCDVYSVGILLFILFTGKEPFDGNYQEKVIQNLIGNIDFHDIKVSILASDLLKGLLKLDPQERLSSHQALNHKWFQREKLIRELQQQIKSKLQNLNKPKNVKASLKNSFPILQTKSAPQISSPLSFRLKTLPNPQTLERVHLPEIQRRTTHLLPLNSILYGRIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TK56</accession>
    <name>A0A8S1TK56_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0210410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TK56"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035843959" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8151546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="284" mass="34332" checksum="2123A8B23E6C2F83" modified="2022-10-12" version="1">MSNNKFLFRTVILIFYSTVQVIELSEEFVKMLEFYKDDYQDEIDIVCYNEFCKEFRLNCFKCQKKGRIHHDHFDDVEKINSLIEFIENKNKECDNLIDDLNQQEESVNQSFSQLKIGIRQKYQLYQQKLILLNSQSINDFLNSTIKFNEYKQSITTIILEQNKKLTNSFNNLYQQLLLSSFNYYQNDNNCIQISEELYQKGITFTQVINQRINLVYYYWLYIKKISMNRPLQFQINQYNKIQKTIILYGVKGIVQDYQMNIKKQLKILKFSINLFNRYIINGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TMY0</accession>
    <name>A0A8S1TMY0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Potassium channel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0240147</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TMY0"/>
    <dbReference type="OrthoDB" id="6128189at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016286">
        <property type="term" value="F:small conductance calcium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000443">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015449">
        <property type="entry name" value="K_chnl_Ca-activ_SK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10153">
        <property type="entry name" value="SMALL CONDUCTANCE CALCIUM-ACTIVATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="145"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="282"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="3">
        <location>
            <begin position="254"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8153323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="462" mass="53582" checksum="9F427B9861AF8145" modified="2022-10-12" version="1">MKGRTGGLTAKMNNQNQNRRQQENDQLVRKADQNKISLAEIFMANSQQLGGLEDGKVTKMYFHKFLFMEQVCFAFNLMGLGISVIQYDLEYEEFTNEDPMKDISLWLLWLVFVSSCLLVFLTVYRYQAHIEWMKSRKSMAPGDQIWHTDDWLPMLIELAVYCIVPLPFTKGIRVNFYNPIQGSEAYYHVNEILALIMICRVIYLYRTLLTLTFWYNNRTQRVCNLYACESNYMFVAKSLLRTMPYTALFIALISLICIFGYAVRICERPLSRNDPTSNNLGVFQNALWNIIITITTVGYGDFYTRTDLGRFVIFIVCVLGIFVVSVMVVTLIESLKVTSLEGHAITVLERVALREKLKHEAALVIILTAKAALGYKNGTLTRKQHHQLIVRLKQTLINFKMTHRQHKTKQDENSLNEEITNQFSLLKNDFKQLMERQQVLISTNNLIYQKLGFDNNKSIVNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TNU1</accession>
    <name>A0A8S1TNU1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0270229</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TNU1"/>
    <dbReference type="OrthoDB" id="313075at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="84"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="193"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8155661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="387" mass="45350" checksum="96481ACB3BE11825" modified="2022-10-12" version="1">MNQGKKHHFQDKNIEIVWTITQDLPQNNQKQITYELIQVQPNENIGYGDGQGQKVKLSNGQEFPSGQDQQGQYNIFVDNAWVPLEQKLAEFQQQIQQQKQTNNELLLYVEVKDYEKFNEYRGFITAKDYLSQTQDLGEELEDLSSEIDKHIQSIFDKNSSVYLKMTNENAELSIKLQLAKGKKLIEFMILIPLVLKEQNKEEKEKLIKDMELNTQKQRLEKLEQELKQLKQSQQQQNQFKKVTMNDINKVQKQEQELEKNIYISREKLSGQFEFELLIANDEIEFQVGIIADDDLGNIEIKKQSYYVILPDGKFARGKVEFGNFFTYKLQVDDKLGLRVDTVKKLFQLVINDYPFPPVKISESIEQFSFIIISSEELNIFAQVPQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TQD9</accession>
    <name>A0A8S1TQD9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0260229</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8154935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TQD9"/>
    <dbReference type="OrthoDB" id="293328at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8154935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="276" mass="32853" checksum="E6161DBE80877A50" modified="2022-10-12" version="1">MKYQIPMELAVNKDCRKTQSANKCRGAESPNKLYQNFTHKNSSQPKVVNLFRNHPNRYNQYLQTSIDVHEINKSKKSVSSHQRVKTEIDKPNDFNKRFYPNEIQMFLNKVEIQKNKLNVLNKTITNKGNRLQAVKVNQGREIYDCNNSKQSSQPDLQDQILRKTNNYLRNNRNGQSTPNNIDENPQFNYFQHKIEYRNRRSNYTRSDDVTPLRRSVFQQIQDYSDTKLDQINVTKEEDIMQFSFGLQYNKLRALKRFPKDIFYGDVRKAKKNLQMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TU55</accession>
    <name>A0A8S1TU55_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0300098</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000030">
        <property type="protein sequence ID" value="CAD8157331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TU55"/>
    <dbReference type="OrthoDB" id="285979at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035882138" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="467"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8157331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="528" mass="61897" checksum="4C7E7CE956BFCA4D" modified="2022-10-12" version="1">MNKIIILSLMTAYVVANNFNYYRTCSKAVCAVPYQTCQNDHACQQTLAICEEKCSSGQENCLETCLFSSNSLTVQKLALCSLNNMCMNSVLCQNDITCPITCKEPAKLGNDKCSTPQAYATQECISEFLIQIGREECAERNCVCKTVASLDIFIAGCNNLSIKKDNVSIIRVKRIIRNYSLSIELNKVLKEKNQNIIYHLRLKMNKKNIELVEELRQRNSRLIVIKYYKNEQAIIIVSFVAYIRKIKILKNNFQAQLGISQIEQEFNLICYYLQKNLQKELSNKETILGRLSNGLDDANILINQLKKNIHDIEPNLYNKIIEQSQINTDQKIQIQIFEQILLKQEIQIQNLVNIQELKNQIQLYWKKIRGKFLQHRLILKNQLLEINQLETPAKRFIYKANEISDIEVQVYKYFILTLSQPQETFKIIRYQQDLQIYKKIILSILTDHFQLHLKLNYKTFKQNEITLIIYFIIFTYLKIIQMLIQVLIENRELIYIYFLQIRCNDNDNLCYIQIKKTINSMSCEIQAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TV56</accession>
    <name>A0A8S1TV56_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0260216</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8154909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TV56"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08215">
        <property type="entry name" value="STKc_Nek"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000631">
        <property type="entry name" value="Serine/threonine-protein kinase NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000172">
        <property type="entry name" value="serine/threonine-protein kinase Nek1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="4"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="397"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8154909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="600" mass="70522" checksum="F4196605EF10CCDB" modified="2022-10-12" version="1">MDKYKKIKVVGKGSFGYALLAQALSNKKNYIIKMIDISKMDRKQREEALNEVHVLKAMRHPYIITYRESFMEKKCLCIVMDYADGGDLYGKIAKQKEIGILFSEEQILDWFVQMALAMNHIHERKILHRDLKTQNIFLTSKNDVKIGDFGIARILQHTYDCAKTAIGTPYYLSPEICQEKPYNQKSDIWSLGCILYELTTLNHAFDALSMKELVLKILRGTYPPIPSQYSSELQSLIADMLIKDPSKRPSIKRILERDFLKQRIGSLLVSTLNRHELQELTELKPTENLFIQPQQTAQQFILQQKQFEQNKISQNQSQNNSPITSRQIIQTVASKKPSPKKDNSKVELKQLFNRQNSPLQSINQNYSIQQQQIYSQQQQQQLLDEKFEKPQSFASVVSQKENQQQQQQQQQQQQQQQQQQQQQLQQQPIKYQNLELLNKFYLKDNTNIVRLQSKSPILQEQKRKESIVSLIREDDSFEQQQGYKFLIKDMRKCLDKKQSKEEEESIIVDNFKIVPQFLNQQKQFQVPGTSERDTIGYKIEALRYYLELQMGLDSFIRAYQTLENSQESEQLKQANQLLNQEQRKYIPLIIQLIVCEDSYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U2D9</accession>
    <name>A0A8S1U2D9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0300245</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000030">
        <property type="protein sequence ID" value="CAD8157589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U2D9"/>
    <dbReference type="OrthoDB" id="354346at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006171">
        <property type="term" value="P:cAMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627:SF12">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0115">cAMP biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="594"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="623"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="655"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="680"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="710"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="12">
        <location>
            <begin position="24"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="12">
        <location>
            <begin position="805"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="210"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8157589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="994" mass="114706" checksum="5A699065F0A2F94B" modified="2022-10-12" version="1">MPRFIQERMNKGQIQISQDQGDVSVLFCDIYQFDKVIKDQQEKIIEFLDTIYRAFDQLCQNYDLQKIETVGKTYMAAGGLKDYDVVINQKNINSTSRALETAIQMMETVKTMKYGDNQDVQLKIGIHYGRVIAGVIGVHKPQFSLIGDTVNTTSRVCSTGEAGFITLSEAAYINIKDNTKYQFEKRSVAAKGKGMIETFKLILQQKESTRNLNPKVSTSENQDKNSIQQKENKKILNFKSSTDKKNQFKRPSIQPAQIGALNLNDHTKSPKVVIRQLHRETQSSIMANMILKKKPSIEIDQDSKRLNTIKSKSFNQNDEIQMKDINHSSNAKTPGFQKNWIQKRKSALDNLLPNAQTQPNNQQDSITQIQFQIDPKPQVQVVSQSCGQQIFQSGVNLSHQPNQQQFLNTADQLLKVSDQQIDNNKQEGFNFIHRPQIKKRGTIILGELIQKKGGIIKSSTKIILPEEREKNQIVKISGVGMDGDSQMEQIKQIRNQRSLKLQQLQDSNSALKKSPESINERLEEIEIVKKKMIITPEQKFDYQTLEKIKKFKLDYDENSTFEIDNSQFKNNDQQLYHSIYEEYKEQEKIQMRNVLVFLTTLNLFKGFLLFIISQQFYDTQTELLIVQICQFGICLIFTISYQMIQDRMNLEILKIIIWIYFLSSSSLSLLIINYEKQVEFEVVLQTISITVLYINIYLSQILNYEDRQRFIAMFIIIITIMIVYEVYILEIIIYLLGIAGLTFFCQFQEKELLFKNYLISLQLSTQIAKYENMLQYLMPPHALRRLLNPDQENTDTFMDVLENTTVLFADIAGFTKYSSSVEPETVVDMLRNLFSNFDQYCQKAEIYKLFTIGDCYVCMGVLDCKKRDPAGEAQKVLAFGFSMIEIINHYKKDPQYQHLNMRIGVHTGRVLGGVVGTDVVRYDIYGEDVTIANLMESSGQEGRMLVSEITKNIVESEYEGFQFDYAKDVYLPSKNITISTYYVQPSDVEQSQND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U5Z9</accession>
    <name>A0A8S1U5Z9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">40S ribosomal protein S15a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0330141</name>
        <name evidence="6" type="ORF">PPENT_87.1.T0390198</name>
        <name evidence="7" type="ORF">PPENT_87.1.T0520179</name>
        <name evidence="8" type="ORF">PPENT_87.1.T0850193</name>
        <name evidence="9" type="ORF">PPENT_87.1.T0950087</name>
        <name evidence="10" type="ORF">PPENT_87.1.T1080101</name>
        <name evidence="11" type="ORF">PPENT_87.1.T1130050</name>
        <name evidence="12" type="ORF">PPENT_87.1.T1300139</name>
    </gene>
    <organism evidence="5 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000052">
        <property type="protein sequence ID" value="CAD8170151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000095">
        <property type="protein sequence ID" value="CAD8190011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000108">
        <property type="protein sequence ID" value="CAD8195053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000113">
        <property type="protein sequence ID" value="CAD8196601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000130">
        <property type="protein sequence ID" value="CAD8202290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U5Z9"/>
    <dbReference type="OrthoDB" id="291200at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.30:FF:000001">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01302_A">
        <property type="entry name" value="Ribosomal_uS8_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_uS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047863">
        <property type="entry name" value="Ribosomal_uS8_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_uS8_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003115">
        <property type="entry name" value="PRK04034.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S15A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="Ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00053">
        <property type="entry name" value="RIBOSOMAL_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8159442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8163394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8170151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8185633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8190011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8195053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8196601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8202290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="130" mass="14864" checksum="4E6C604CB9E00C3D" modified="2022-10-12" version="1">MVKMNVLNDCLRSIVNAERQGRKQVLIRPTSKLVVKFLQVMQRHGYIGEFEIVDDHRSGKIVVELLGRINKCGVISPRYDVTLGEFERWANNILPARQFGCVVLTTNVGILTHEEARQRHIGGKILGFFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U6G0</accession>
    <name>A0A8S1U6G0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0340292</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8160350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U6G0"/>
    <dbReference type="OrthoDB" id="302658at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053083">
        <property type="entry name" value="TF_kinase-domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44305:SF2">
        <property type="entry name" value="SI:DKEY-192D15.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44305">
        <property type="entry name" value="SI:DKEY-192D15.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="411"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="415"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="435"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8160350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="445" mass="51321" checksum="BA4BEC7016E514C8" modified="2022-10-12" version="1">MGNNMTVIWSEQLKHSFHLYETNLKHNVLGNIDIYQYIPDNKVKIFSKTAYVPDKEYFIQKTKFKHPNLLRALALEKCSGSCSSDQDLYKVYYDYPGICTLEQIINEKIKKFSESAIWNIIFQVVDCAEYLQSHYKTIGNINPNSIYVLNDGIKILEVNHIFQIESSYEQALKYSIAILSPEQIEQLQRDIPIPSVNGFKSDVFAFGIVLLCLTTLSRYVKFYTQNQELDKNQIYQSLQQTKNQYSELLYGLIQKMLVENPSERQSWIDIKRFIDPFKILEENDQPFYSDLKLCPQIKQPNIHASEQIKLNNQISQTQVPIVISPNKTTKIIQSSQQLQTVPIPIQNPNPNPTITQIYAPSSFSFQNQSMQQSIDFKLLPNKTANFAINNSNQPIGNASMNETQQVPFSLKNQNSQQTTPPQSQTPYSTVKYPFYQSQSEKPSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UCD4</accession>
    <name>A0A8S1UCD4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0020350</name>
        <name evidence="3" type="ORF">PPENT_87.1.T0380299</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UCD4"/>
    <dbReference type="OrthoDB" id="299305at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036434461" evidence="1">
        <location>
            <begin position="17"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8133332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8162901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="359" mass="40273" checksum="B264C6D540113282" modified="2022-10-12" version="1">MQKLAILTLVLALAAAKFVDTHASLAQIDANPFGNVVLSAIKAHLQAQTPANEVNMLLNGVAAGLQQDQNDHDHAFELDTTTNNRIVEDLEKEILYHQNQISSNTQLRDDTIEALAVSEEDIRVTISDIATNEQTYAREEATRNQQHETFVAKVAAIDDVIDAIDDAAKLIQHLSLGASFVQVKSKYETVHKRLTDNTSHSQLLQPVVVALTELATHGVNQKALTKIAQLLSEIRQQLVQEKAVKTDVEDRQAAHWAEFSAHLSNEHTRLVERKAQLEVQIQEQKDTIEDAQSWIEFHTLELENSEERLAGQQAWYAVQSEIYETQTAERAAQNEIVDRLQEHISEKLSTTAQFISKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UMI6</accession>
    <name>A0A8S1UMI6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">adenine phosphoribosyltransferase</fullName>
            <ecNumber evidence="6">2.4.2.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0410283</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:16609"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.4.2.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.7" evidence="6"/>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UMI6"/>
    <dbReference type="OrthoDB" id="363185at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002055">
        <property type="term" value="F:adenine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003999">
        <property type="term" value="F:adenine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006168">
        <property type="term" value="P:adenine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000021">
        <property type="entry name" value="Adenine phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00004">
        <property type="entry name" value="Aden_phosphoribosyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005764">
        <property type="entry name" value="Ade_phspho_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050054">
        <property type="entry name" value="UPRTase/APRTase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01090">
        <property type="entry name" value="apt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002634">
        <property type="entry name" value="PRK02304.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002636">
        <property type="entry name" value="PRK02304.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32315">
        <property type="entry name" value="ADENINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32315:SF3">
        <property type="entry name" value="ADENINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="11">
        <location>
            <begin position="45"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8164902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="176" mass="19669" checksum="4908DA581E417DA4" modified="2022-10-12" version="1">MELDISQLVDKTVRSIPNYPKPGINFRDICPLLKNIEVLNKVIDYFVAKINFEFDYIAGLESRGFLFGILLSQRMNKGFIPIRKKNKLPGEKISLEYKLEYGSDIIEIQADAFEPGSKILICDDLLATGGTAVAANELINKAQGTTVGFVFVIELKDLKGKERFDPKLPVIVALCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UMR7</accession>
    <name>A0A8S1UMR7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0440099</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UMR7"/>
    <dbReference type="OrthoDB" id="297910at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035902057" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="534"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8166340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="593" mass="69513" checksum="1774217F528D2C3E" modified="2022-10-12" version="1">MINILLTFTILVFVQSQIKLENPIKDLKIIEVEKRDEQQLIEMIPEQTKRVVQYNDSKTTIGMKVTSSNETLLKCLSYDKNILFEPYMVNEIKLLFPSINSYYTDESLKINRTANVNDPDFIMKNPVLVGLANTTSQGFIVLTSNHLLYLFQLSFSQLSNKFSIKSNQTFNFSDLRQTQYEPVSPQLIWFDQKSCVLIIFPQQIIIGKLIDGQFLFKNSNISISNEEEYIGIAKLHKGYLFVARGRLGIDIYSINEQGQLIQIQNLYNQLSNDIHNMYIQDFDFGGFDNNYIFILDKYQGFMIYQIDNQLFTLTKWLDSILEIKGAKLIKTYINIIMIMVEHNSYNHILEYCIYSQDKKLFYIKTHYLDGKYFDIDIEDQFAIIRGYDGHKIIYHSIHEGFQVNYNSPITDESWEDGTELQEDIDQYAIIPQMQSFYLTKHLNQSFLVAISFRTLMFMRFIRVEPYVECKLTNLATKNSQITVNAVLNSTWCKEKFQQQIVYNQSFYSDLIMCKKELDFSIKVINTLEEEQTNYYYIIIILIASLILLCFLLIALRIFFAKYRQEQSSLESVIDQLGKRGYTAEKSQDQISGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UPX5</accession>
    <name>A0A8S1UPX5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0460036</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8166725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UPX5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8166725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="122" mass="14656" checksum="3312DD1A42BF6782" modified="2022-10-12" version="1">MFIYNKYKSFWSVYYSLQENFTIFKTLKSQIEKYSYFQIFTFIGDNKRICIYSINSNPTQTSKFIEYEKLIELQNSRKSGKGSTLDIYKKSKELYQQFTINTRPGKSFEIIQKNGNFENIFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UUB8</accession>
    <name>A0A8S1UUB8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Laminin EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0500001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8168808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="293546at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035714904" description="Laminin EGF-like domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="1300"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="2">
        <location>
            <begin position="203"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8168808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1300" mass="152070" checksum="179EE253CAD232BE" modified="2022-10-12" version="1" fragment="single">MFKKFSRIYLSIILVKLVYCSCEVFKTITTPNTGQRLIEQDLSSLVTLDYAWGVWTKYSKFYVSKQLYSEYINPNLWYLNPRCTTDYLMPFSNDTNYVFLKKDRKIKDFEYYLINQLNITQQPVLESCFGCLEQTESSFNFIEKYTLNKIQKFLCKSCYQSNNGECVKVNKNCNECNNYHQCISCELNYTLYDNRCLECPLECPNCKYNQTGYYCRECITGYYFNQTLEQCQQCGQFCKVCIYSSTLNNLQCVRCIHDQDYFISAEKLNCKTKTLPNCEIEVSEHYLVYFNQSLMLEAQQYSNSLDIVTFKETIPVFQVCLKCKNGYINKMLVQQYYECASVESQGNFLPKELKLKILESKNNDFQLALYSLPQNPQLKFVIIYGDRVNSYSSITTISNPITGIFCNDPNCMVCVNNYIQEQQYCMQCLNGFYSNTFLGKCYQCPSTCSSCIEQNKVYQDAWKWQYKINYHFRNYLLDGQHNFNVFGTKLDIENLEVICTACPQNGILYNNKCYPKCNCDSCIIENDQSVCISCNSLNTKTVYHGQCHLCPQFCELCREITEEEKLKINPYFQPDDITLQKYAQQCIKRQSEPPKSGILYYDHTLGYEINCLNSEKKDCYLYLEIPINLYCSKSIFDYYFYRLETSEEQNEFLSKNALISEIFSTLNINHFSVETNYLFNELNQKSVKLVRYILNVLPKTGDCIIKKNTFIKSEIRRNVFTVQYLELILQSPSSRMQIYLDDNITFYEFTAINLKNLQIIPNNNTTSLNIKNLYSISVLLEEIQIKNINQNQFKIAIQNPNNIKIKDFQILDSKLDNLNGIINYYFTQNLTKTLVFQIDQFIISNCFITNSKLIVQILDTGYNNQLLKVNNFKSLNNKYISSITFYTQFNNSNRESIFNIEQFNSEHDELTNSSLFNIPGVLNVELINIIVSNGKFNSQVQLFKLPLFSIDNKLISNNNFNSPDNVVITNKVDALYKDNIKSNKVSLNSITFDNNNYIGSKVFLELEQSDQFFDLKVNINGLTLHTNKFTQQNYKGSVNSANSSLYFDVKEIYMENVNIIRTFTLPEISIVNVDKIVFKNIRATLDYEFKIKLFHQFVSCLQQNQDIGYGSMIQLYNVKLIQIDKLEITGLIIINLPIITLKSLERTNFRQSEKIEMKYLNLTNNTIILTKLSEQPCVISIISEQQQQIDLQDIYTFNNHHHTYQEDQLFKQSSTIYINSPNSKIILIKSIFENNIITNNQGSNLVFKCGNFQMEDCYFNSQNDLQYQYLKDRLIWGYQQNEIIFIENLHSIFPIKSKGX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UUQ5</accession>
    <name>A0A8S1UUQ5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0440001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UUQ5"/>
    <dbReference type="OrthoDB" id="316578at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8166176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="94" mass="11027" checksum="0EFE452643787909" modified="2022-10-12" version="1">MISLSVFGMQRQDKKLNPLIINIKIFLPSLKYHYRIAHYYQMPDRTILRICQNPLLEARGTLILQGEFINQSGIDLKTLFKQKGSCFLEDLKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UUW8</accession>
    <name>A0A8S1UUW8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0440043</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UUW8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8166246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="92" mass="11168" checksum="58F0FBEA9C330FBB" modified="2022-10-12" version="1">MLTKVNLNKCYTIQHSQNSQHQQGIFLGMKFNNYYLIFLEKVQNVINNVPIFQGLVQIYLHSRIQKQKKRYEQLLKRCGFRRIIKRKKRITV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UV24</accession>
    <name>A0A8S1UV24_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0480189</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000048">
        <property type="protein sequence ID" value="CAD8168103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UV24"/>
    <dbReference type="OrthoDB" id="294710at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000445">
        <property type="term" value="C:THO complex part of transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021861">
        <property type="entry name" value="THO_THOC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13265:SF0">
        <property type="entry name" value="HPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13265">
        <property type="entry name" value="THO COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11957">
        <property type="entry name" value="efThoc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="509"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8168103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="536" mass="63762" checksum="460D8F1DC99F9262" modified="2022-10-12" version="1">MSEFKQMEIESYILNFKARHTEIIKQKLLKLHEEESADQFQPFYQSFLKNCRDYLHQKLKLTCKRVLDEDLLELLKVNIYLAELSYTICKEVPELKPYPLTFSEDIYECLSLSELYDYFNNLYSQISTISKFQDENKYVLLRICNSMLKRLSTTHDTFLRGQVQIFLTQIFSTIHPSLRKKPINIRDRGFDEESVKVNASLPYSFYKNFWTLQKYLNNSELIFRNKVEFDEVGEEAGQVIQGVSEPQRTKNLQAVIKVIMRALQYFKENPLESQIITVKRFPKFLTKYSLFKNQLNDPYFRKLFLNQVLLFIFVAELENHEKQFIQNLKIEVQKQLDELGDKLGEKVQHLIDTERTWRLWVTEKQCFDDGYEKLSIKASEKAKKKRLEIQKRIYDDLKYKQTDAFQSILQDQGRPYKIETNPIEPGLNYYLNEVFYQLDQTRKIQDNERCKNLKDYAWKSVRVISKYLLKELKITNLITQDLKPLPNLEDIVENLKNAGNEMTAERLQQSNNNNNNNNNSVTIKPVHISQQLPNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UWQ1</accession>
    <name>A0A8S1UWQ1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0480127</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000048">
        <property type="protein sequence ID" value="CAD8167979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UWQ1"/>
    <dbReference type="OrthoDB" id="62956at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035751139" description="GOLD domain-containing protein" evidence="9">
        <location>
            <begin position="17"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="165"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="10">
        <location>
            <begin position="25"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8167979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="196" mass="22930" checksum="B99538E09F6F0115" modified="2022-10-12" version="1">MKYLLFISTLIVLGQSLSTQLSQKPTCFLVNSPEENSNLTVNYIVSGLDEIQTDVFIRKKDGEILTKVQNTKDGKIREILKDKDNYYVCFQSRDKSYKMVSFDFDIEGVDKEYAQTEQFEYMSKELQKTQKNFQNIYRNQHWMSDRENAHAIILDQTEKSVQWCAVIKVGVLIVISISQIYIVYNYFKDKDLNGQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V6T1</accession>
    <name>A0A8S1V6T1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0570163</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V6T1"/>
    <dbReference type="OrthoDB" id="301111at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8172574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="197" mass="22834" checksum="1DFA49DD4A1D5F23" modified="2022-10-12" version="1">MDIGLKKKSLKFHVKSASTSNMFASPQSTNTPVTGKSIAQFVHIPFLSFKRSEMKKYIQTEEREYLKKKQIIESGYLTQRGMSKFLKPNRLKWQKNDLKIHDNILKTKLNIADIILNAKGGQSRRELLIEKIQNQIDSPQQIISQRTIKSEHTVQIFMDRLKFQSLPQKLKGDSQSPLKTIQSQKFIFNSSTTYQTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V6U4</accession>
    <name>A0A8S1V6U4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0530073</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V6U4"/>
    <dbReference type="OrthoDB" id="297207at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="84"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="120"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="745"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="927"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="746"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="759"/>
            <end position="774"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8170466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1031" mass="120476" checksum="BC37161566AFF3C2" modified="2022-10-12" version="1">MLYKFKEYFVIYQEHLKNKDQWRLLMVLISVYQICDVIIVTGDAVTLDIEGDKYILIYRILTQIFLIAFFEVSLIKMIKIKNGFINLMTLLKFICVVICWEELDYYTTQQLFQRILDKFLILLGFVFAIESQVCQTLAIVFNLIYTLLRNFEFDSKMNIIMGTKLIVIHVILQFLMFIINKKNIRNNLNSQLVTMGLNNKISYTQLNSQRLSNPNRTLHQLYIDIPENRNISQIPHEQKQEDQQTESVQQDQINEAILSTSEFGIYLIDSLTKSVQVINPQLSNIIMKEDQLNTQFLETELYDFGLLLEESCLLLPKFHRSQDIAQFLKFTETLEYFPSTLENTYENDPCQIKRLQLLTKKISFKAFFDNLIAFIHLKYTNNHLLIDFSLKIYIKFDTCYIQVVLSPLIYKLKPQIVIFVSDITEDPYITQLLNTTKNNDFLISDISQKIKQPLNCTISMLEIIMNTTPQEIKEKYISPALAGCKLLINTANDILDYAQLQKKDKLDLIQMDVQIQEFLTDIINVVKSQAIFRGLKISVNIKQNVPQFIRTDPNRLRQILLNLLVTSIQATVRGSIIFCVSKSQILNEHIDFIVKVKANETNFSILKIIERTVRFFKSQTLKSKILDLGNLRQYSQSILISFYLTKCLSQIPFEYNYERVGNKEEFSFVIKIKNLYPQFNQNLNQKRLSEFTNQQSFYQQYQNKEGLKRYQSQMSSLQPENSNIKLELKEARQKLNINIIQTQGQESKEKQKEIQEQLNESDESLSEQQDDEVSMESKNGISNRVEQMLKIQPYFFDYVNETQQKNEGISSQPSQQLTFGGLGQGRSSIYSSLCFTSGTMQPNDLLDCFEKMEKIKQQKNKSNCRCTKILICESIDLDLYALSHQLTNIGITYEYVTQKSQIVSTLVKLLNSDLQQQQNSNQQKQNIDNHQLKVNNDNQQLKNNENQPCCKGLQLLFIGIEQFEEELFALFNQIKDIYAEFKVEPRIIGLIGCMDEENRAQTRKLPFHDYLSKPIMIDALLFILAKWIKMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V7B7</accession>
    <name>A0A8S1V7B7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HECT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0550166</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V7B7"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000009">
        <property type="entry name" value="Probable E3 ubiquitin-protein ligase HECTD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000024">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF440">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NEDD-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="4">
        <location>
            <begin position="142"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="2">
        <location>
            <position position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8171719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="468" mass="55242" checksum="C98ABE27FA875A8C" modified="2022-10-12" version="1">MGNRSSNNQHSKIGQETKCPNCDRKFGPSTTYFVLNSHIDHCLLHQQPIPNIEFQMPQTLDQLGDNYIWVKQNNQWKRILSQVNGGQIQNLSVQEIKNRSFPEKQMWFNLQLEKFRIPWQLGSDKLNVNLNDLLQSSLISARSVNFYKEVKVVFQNDKVQDAGGLLREWLTLIFKEMCKDIFTLTETNEVTYKIAKQSQYFDLAGLAIAKALFERMTICVEFDRPLVKKLLGQEISFQDMQLYDKGLFLSWKYLLENQFDENQLQQYFIICKDDEIVELKQNGADILVTNQNKQEFVDLSIQFYSEKLISKQLIQIQNSLYKYIPKDYLNIFTAEEFEMILYGVSVVDLTEWRQHTTYKTPYAENSQQIQWFWKILSEFDQDQLKKFLHYCTGSYRIPVNGFSKLESNRGMYSKFQIVPIDYKNANSFPIAHTCFNRLELPKYTTQEMMRKYLRSIVLNDLEGVFGME</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V7F2</accession>
    <name>A0A8S1V7F2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0590028</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000059">
        <property type="protein sequence ID" value="CAD8173270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V7F2"/>
    <dbReference type="OrthoDB" id="10263032at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.1040:FF:000009">
        <property type="entry name" value="N-acetyltransferase subunit Nat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.1010:FF:000009">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021183">
        <property type="entry name" value="NatA_aux_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22767:SF2">
        <property type="entry name" value="N(ALPHA)-ACETYLTRANSFERASE 15_16, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22767">
        <property type="entry name" value="N-TERMINAL ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12569">
        <property type="entry name" value="NatA_aux_su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000422">
        <property type="entry name" value="N-terminal-AcTrfase-A_aux_su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="226"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8173270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="789" mass="93597" checksum="8A9C00DF5B03731B" modified="2022-10-12" version="1">MRPAQTSLNAKQSGEFKALVKLLDAKDYKKGLRNTEKLLEQVPDNWEVVSLKAIFMYYNDEQQKGLELAKQAVFKNLGSDFCWHIYGLIYKAQKNYIEAVKCFLQAIKKGEENIQLERDTANLQIHTRDFEGNAQLRHKILTKKSNMIVNWIAFIFAQHLSGNFEIAFKAINSAEQLIQKDQQNPIKKVEMNEFKLYQIQLAIDAKDYQKAKQNISAFKNDITDMVAYYELQYDLYMKLGSNQEAVEAAKQLLELQPQNWRYYQLLKKADPQYDLSIYDNNLVQGQLLAEQSGDTFKTNFLKFIDPFFQKSLPSLFREIKHLYKQNEKLEIIREAFESYLNAGPIQKLWGLMLLSQHYYQIKQYTKSLELVEEAIAHTPTLHELYLIKAKALHKQEKFKEAYEAADRARQLDLADRYLNNKTIKYALRANMVYLSQELLRMFLRDGSNPYELQMIWFELGVGRTLLRLNYLGPALAQFYLIFKHYQEMYDDQLDFYQFSIRKYTLRSLLQMFDAMDTRYSAKYFIETAGLMIEGLNRLKLKQQDQGKIEQKKLTPKEKKQLQKLQQKQEEEKHLREQFSIEGHIYSIELQKKFDLSGEYLLSQLKTEQDIIRMQNQFAQVLIHTNYSNKEINFQAFKQLVSFYIQQNRPLLSVKLLNKLRNNQTEQNKIVNHKYQLMLIQYLNSYKGPQLQYVQEYQQDLNKFDTDFWKSVEIKSNLDKTIKTHCEHILQNKPFNVQQLESNDLEFLQEYPTAKTTNPFYTNDPNLEWQNNQISQFQSTQQTQKVSQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V9E7</accession>
    <name>A0A8S1V9E7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0570080</name>
        <name evidence="2" type="ORF">PPENT_87.1.T0570088</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V9E7"/>
    <dbReference type="OrthoDB" id="10255522at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8172412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8172428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="60" mass="7355" checksum="7BE20DF30632E1CB" modified="2022-10-12" version="1">MKSIVFIIQADSKKLYDYWWQKHFQKENSRVINQIYDYATLVQCTKLLYVNMSVTYKIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VAN6</accession>
    <name>A0A8S1VAN6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0590193</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000059">
        <property type="protein sequence ID" value="CAD8173573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VAN6"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8173573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="109" mass="12900" checksum="F78E6EB5CC296228" modified="2022-10-12" version="1">MIRVNRELNVIYSIQPLGLLLNQKTNIQTLKNQIRTHLKGFVTLFFSCRTQNDYLIKVECKEYEKMKGKTVDIIINKDHFGLGVSLQITFSNMMDLLMYKDLIRWVEML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VCW3</accession>
    <name>A0A8S1VCW3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0620145</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Plays a fundamental role in microtubule organizing center structure and function. Component of the infraciliary lattice (ICL) and the ciliary basal bodies.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the centrin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8175050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VCW3"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000178">
        <property type="entry name" value="Calmodulin-2 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF59">
        <property type="entry name" value="EF-HAND SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="63"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="99"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="135"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="171"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8175050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="208" mass="23287" checksum="0EC48A21240C505D" modified="2022-10-12" version="1">MSRNQSRQQSAAQPTQQTSKNQPPAPAPAPAKGKAPAPVQQPVQPQASGFDASKYVKPGLSKEEVLKIKECFDIFDDDKSGSISPNEMKNAIIALGMEQSAEEIVNMIQDLDQDGSSVIDFEEFLNIFGFSGTIEDEQVLEKLYQEFDSSGQGKVTYEDFKRINDLVSERYTDQELREMVEYADKDKDGSLSWDEFKTVVQKEYPNQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VFF2</accession>
    <name>A0A8S1VFF2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0620195</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8175145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VFF2"/>
    <dbReference type="OrthoDB" id="288796at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001816">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="17"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="411"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8175145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="465" mass="55121" checksum="5850D4311A8F68AF" modified="2022-10-12" version="1">MDQNNSLQPGLIIDQRFKLLKKIDSGSFGIVYLTFDLDEKEYCATKFENRNLQLRMMNREILILKQIKGINGFPDIIHNGKDRQYSYYMSTLLGENLEVLLKRCGGKFSLQTTLQLAIQLIDRLEVFHSMNFIHRDIKPENFLISKEDTSLVHLIDFGLSKYYRVADGKHIEFSQKTGIIGTARYASINTLQWMEQSRRDDLESLGYMLIYFVKGELPWSNVKACNKEDKYDQILQVKMSIPLMQLCLNLPKCFIHYFQHVKSLLFQQQPNYSHLRNLFEKQLQDYDHQLYDWELKQMEKQTQDILIDPNSIPNIRLQSEIDLVPPVDEEGEAFHLKQENQHNVKFMKQLHEKYFDHNTFKQQDEYQLQKNTSIEPKSQATSKQNNYQISNHTLIQVESQPYIQNNNNQFTKRKDSKSYTSFKQSQEHKENRKFRQSNDDYDLDMANDDGPNLDLKNLEVNYFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VG59</accession>
    <name>A0A8S1VG59_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0630043</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VG59"/>
    <dbReference type="OrthoDB" id="306758at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8175413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="77" mass="9148" checksum="0AFDCFB6BEE5C7F5" modified="2022-10-12" version="1">MFLRLASNFIRQPIMMKQISKQLTFLPMTTILNNHKLLIYNLSQLHTAFFQIEEQNDDDDLLLEEVPVRRTNSNQLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VGF9</accession>
    <name>A0A8S1VGF9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Profilin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0630108</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the profilin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VGF9"/>
    <dbReference type="OrthoDB" id="309229at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003785">
        <property type="term" value="F:actin monomer binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048278">
        <property type="entry name" value="PFN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005455">
        <property type="entry name" value="PFN_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11604">
        <property type="entry name" value="PROFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11604:SF0">
        <property type="entry name" value="PROFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00235">
        <property type="entry name" value="Profilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00392">
        <property type="entry name" value="PROF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8175543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="156" mass="16810" checksum="640DC1AD9BE029E6" modified="2022-10-12" version="1">MSWDAYVTNLTANGALEYAAIIGLDGNIWASNLGVAALPSYQADVPDEKNPDVSTKVAYDEKTAFVHALTHNGESGNKAGVRINNQKYYSVQFDAESKTWYLKKNKGGACVAWANTVAVFASFSQTINAENGAPQNASDCNKRVIDMAKYLADAGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VHE0</accession>
    <name>A0A8S1VHE0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0640090</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8175943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VHE0"/>
    <dbReference type="OrthoDB" id="10254720at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024977">
        <property type="entry name" value="Apc4-like_WD40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042234">
        <property type="entry name" value="WDFY1/WDFY2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46189">
        <property type="entry name" value="LD41958P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46189:SF1">
        <property type="entry name" value="LD41958P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12894">
        <property type="entry name" value="ANAPC4_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="PX" evidence="2">
        <location>
            <begin position="5"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="400"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="430"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8175943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="473" mass="55561" checksum="432949BF849B9105" modified="2022-10-12" version="1">METQHKQLDVSVFSYKPDFSDKSKIMYNISITFENQSWIITKRYSEFDDLYKQLKVCFRNQLPELPKKTYTSFLFSKTHDDIEQRRIGLDKFLKLLTSKQEILSSNQMKEFLKLEEHAKTLVVNQPQIIHEFSGFLHGVRDFNLCIGQGILFVVNSDYSIFNRLDAYLTNMKGPWEKEDGQQAVMPVGCIECWIQQQDGLYTRQWAKMYNSQAICCFWDPSSFVLLVGLDSGSINYLEVPYSQGFKKCETDAEIQQHSSKVTGLYYDNERSLIHSVSKDKRYKIRNAVKSIYIYDNEISPYELTDLVVSKSRRKSFVSDKNGFIYMFDINEDKVIQLNFINTNIRLLKCLKLDSNQNLLMAIGQETGEIIMIDIGSIKCEKQLRWIGQINTKTKGYKFCWSQKRKELFVGTSDGTVQFWDYDTKQLIFVLKAHDSEITQMQWIEEDQHLLTSSRDKEIKTWLLPLQWKNQQVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VJJ9</accession>
    <name>A0A8S1VJJ9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0670197</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000067">
        <property type="protein sequence ID" value="CAD8177414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VJJ9"/>
    <dbReference type="OrthoDB" id="309079at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8177414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="297" mass="34937" checksum="F3C9ECD413B02BB5" modified="2022-10-12" version="1">MFYTLTIEQRDRRKKDKKANQVNCPQCPASLQGILKSKAFCIFCLNYVCKTHIVKKRKDPLIDDKIGKICKECEDAILFQKIMSKHQKKEQELKDQYQELQNTLDNVNSQFKDLLSQEQTIKYTIEKGQKEYEKKEQQAKQNIKEAEDRQDQCNQESLQLSQQYRKLVEEKQIKEKEKQALQEKHENIQQQEQQILIEIQEIQENIKTVNETISNITQEIARINYNQKSEEKEKQSLFARYTQSGVGTIQIDSKQVSEIIPRTTIQKNKKQILDQPDRGEGFCCMSIIPSNSKKKKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VQD3</accession>
    <name>A0A8S1VQD3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0690090</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VQD3"/>
    <dbReference type="OrthoDB" id="1711136at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047126">
        <property type="entry name" value="RNF141-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109">
        <property type="entry name" value="RING FINGER PROTEIN 141-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109:SF5">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="226"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="359"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8178285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="426" mass="49985" checksum="D6C02E614EC0AFDF" modified="2022-10-12" version="1">MLIFSLKLIWLFDSKYIIIFAWLMDLITFIVILLDKNISKLISCIHFAEKVLSVSFKVLLLHFLYNKITIIIHFEIQEINLYYIPALQLIVTIGLFLHQVLQQNRNKRKHIIVLFKSNQIAYSYSILYILQLLLLSLKWNHLFIYSYYQTFIITWTALGVNTFLIVLLFITLVENCFHKEIPEIDGTILSYQGSIIIWFIYYIFGLTIIPFFLLKEICQFYEKDTIQINIRIAGITVLISIILYLISFIFFTNKIRNQLIIVLQLENRQTQITIPKSPQIETPKKKWSKLKIPIMFIRVSASYYKIVTKKKINELNNRNMSSIPICSDILRPDSLNFKQSPCLTMMKQMKSIPKDQDSCDICLICFENEPDIVLNPCNHGGICTSCSENIMKTTKQCFLCRTDIKYTFKLNQKIGEILEVTDVQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VRU8</accession>
    <name>A0A8S1VRU8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0710013</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000071">
        <property type="protein sequence ID" value="CAD8178973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VRU8"/>
    <dbReference type="OrthoDB" id="283020at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8178973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="147" mass="17545" checksum="2D2D720F80A97A01" modified="2022-10-12" version="1">MINNLQPLFGMYKELWIENATQLKDALEKWDQHQLKSWLQKMERTIFTFQYDECIGLLDEFQPNYIQNLPNDMGIQCNKLRTMFQNNSTQYLIVNTYLKLLRCLKKSLEPIYNSTHLLNSIRNLELYLSSPLNQNTIKPQECNCTIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VUP7</accession>
    <name>A0A8S1VUP7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Calcium-dependent protein kinase 1</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="ORF">PPENT_87.1.T0760139</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23 31">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22 31">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cell membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Parasitophorous vacuole membrane</location>
            <topology evidence="24">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="29">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDO010000076">
        <property type="protein sequence ID" value="CAD8181434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VUP7"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020002">
        <property type="term" value="C:host cell plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020005">
        <property type="term" value="C:symbiont-containing vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000398">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000199">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000695">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 26" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17" id="KW-0969">Cilium</keyword>
    <keyword evidence="15" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1032">Host cell membrane</keyword>
    <keyword evidence="16" id="KW-1043">Host membrane</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0519">Myristate</keyword>
    <keyword evidence="12 26" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0564">Palmitate</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="27">
        <location>
            <begin position="125"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="423"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="461"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="497"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00060437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00068067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="EMBL" id="CAD8181434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="561" mass="64220" checksum="5A0920853E67F8FF" modified="2022-10-12" version="1">MGAQCCCSFPQKLITNEIVIDVKGSLNTLSKKSINNDQLIGLDNVNEIKEQYQDFEVIADSPQDLTPKDDQNGQQCKMFAQSESKVDTLPKNGIKKKLTVSSIGTVKLGADVFVNLKQGSIHKYYTTGEVLGQGAYGKVWKVTHKNTGMIRAMKQLKKSSLIVEEEQRLFAEMNILKNLDHPHIVKLYELYQDQQNYYLITEYLSGGELFDRIKSMTYFSEKKAAEFIRQILLAVVYCHEQKIVHRDLKPENILFINESSNSPLKVIDFGTSRKYDTVKKMTKKLGTAYYIAPEVLKQDYNEKCDVWSCGVILYILLCGYPPFTGKTEKDIMHKVSEGKFKFDQEDWGFISEEAKNLIIKMLQVNPNQRISAKQALHDPWIDKHNLNEKVNQIVLQNLQKFQAKSIFTQAVLSYIACQMTSQQEQDELVKTFQTLDQDKNGILSKDELIEGYTLVLKDKDLAIKEVNKILQIVDLNQSGQVDFSEFLMAAMNQEKLVSLERVKAAFKIFDANDDGKISKEELENMIGSIDEELWQQILTECKAEGEITEKEFIEILLNQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W241</accession>
    <name>A0A8S1W241_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0800146</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W241"/>
    <dbReference type="OrthoDB" id="294930at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="755"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="829"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="870"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="768"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="844"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="873"/>
            <end position="893"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8183231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="899" mass="107129" checksum="434AC2ADEA3952D0" modified="2022-10-12" version="1">MRLNTQLPPKHNFNASFNYDWDLMNKKREIEMLRPREICPKRNHSFHTSSNDCDKSIHNRSFEKSMQETEKVSSRMQNEFGQLKTLIQKRRDEIEKQQRQQDLKLSISIPQQTIKEDIRKQKEETPQFNYYQTFKPIFAQLNSLIENSNTKELDKQRLVTLFDSIDQESIKKSNEPESMFQLVEQLVKEFVIPTLEQQTPTQQNSKRSYMQTQSSNFIKQLPSHQQIPKSIEQSINYKNVFELPYKNPLQFYQQIKNNESGYYEYCINDQIVTGESINNFNISKFQSCTLKNQQSNVKVDYRQIKQSLIQQQIYSLDDQFTLIPITHFNRKVIYALNYQSSYCKQIQQYIQLNISNLQYKLELGINKINKIVLLQQRMKRLMVILRMKQFINQLRKLKRLIQQFGFKRLKQYYYSIRNSKNINNNICKLILQAAKQVKKNERNYTIQLFMILQKYQSRLKSNAIKQLIGNDDLIIMLQALQSENSIEMYLQQVAQLMGEKLAVSILNIEILSLTSYLPEYGLIQEYFEDIDDISQFNTLYQLTLNLNTELTNNLNIEFENRHIWKVNDEIFLITSSNMQTQYLSKIALILTNGINSLRQKYLEFFKFIRLRTEQHQQKMKSKFFNRWRNKQGLFVDQLRNELVCSQQEQDQQQRLIYQYELELQNMQIQNALKSKCLSSAMLENTLSKRENQLSLKQTFFKLWRSRNTNIRHLEQRVQTGLFLLEQAICKIEKSQLDIAFREIAFSYTQPTGTIPTSPILKNTKRSQHTREQTRRSQEELEQSVLWDVHQMKIHSPNGHKGQSLSPITAHTINQSFLIENESLYQYHSQRCTQQPKKKAQQQQSSNLDQENVQSQNKIYSQRTKDFVRKIGQPKQQQQNHRRINSTQPGSSFSFYDLQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W2M6</accession>
    <name>A0A8S1W2M6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0780251</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="290842at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1728"/>
            <end position="1750"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1814"/>
            <end position="1837"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1904"/>
            <end position="1924"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1931"/>
            <end position="1955"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2042"/>
            <end position="2065"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="417"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="708"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="752"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="906"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="1010"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="1072"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="1249"/>
            <end position="1283"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="1310"/>
            <end position="1344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8182522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2109" mass="242447" checksum="8A31986F8B108E3D" modified="2022-10-12" version="1">MIRLLYTIFVLLYPFRCTWIRIGSYITSVKQFSSTDTDGFISQFGNPNLPANYINCSAEGSYITLNTLSQSAIHQNSLSTSSYSMYFITYDLIFFNTWNNEDFVKYSIGSYQNDIIYNNIEKVSISKGFCNNQVAIIQTINFTINSTIISGYHKFEINSLTARVSIKNLIVSALQCHGTCRICFGSKYNQCSFCFSYGTLTSQNTCEYSCPSTAMYYVKNQGCKPTCHINQQKYVKGVCEPYPISTILGLYISSSKSTEQFRWQIIYDFEQLNTVLPENRQFEGFYIYGIFTNNQGIYRVLTLPSNQGSFMIAITVELVLFNIMPPLSSIQFLINDTYYGQIYSDDQGTLKTDKLFRFWTDTRSNTVFNGISYTNNQYNTLYFYADITNTFVIKMIGNYSNSNAGWGLRSLYVYSGYCPSNCLRCEEKFNCAQCQTGYLKSKWGTCSQCLGSYQQKINTTHCQEDDDETNYSKYIIKEFTGLELDIETYPEYTLLNAVGQNFLKGQGIYYSVWKSQFRIFGGPFIWAQAKFSRIINIEDPHHAITISFIVIFGPNFPEDGQFIFSLDGIIQMELTSSFSVEISIRHKQLHNQNSISIELECKGINNEPIYSYCGFYQHYFTVHQCKPNCNSCSNESDCLDSNYVESVPICLNDQYFDQYQLRCQSCPTTCMTCTSFQNCLTCVETFSNSTQGCICASNQYLESNICNNCPQNCNQCINSLKCTECKQGLFRTLINNVCVCQDSYFELNNEQICQKCIQNCLKCSSINQCDICLVGYQLNINGTCDQNPNTYFQHTLDTFLECPVSSICNPCQGINLSCVCGDMIITGDEFCDDGNSIQFDGCYQCKFQCQPECTKCINGVCYECATSGWYLDVSSSTYYCKEVCWDGLIKGKEKCDDAISTTNCKDCQFYCKNDCLECNYENGKCIKCREGLIPENNYCKNICGDGIIVSAIDIPFTEECDDGNLVDFDGCSKTCLFQCQNTSICDDCKNTKCFHCINKYKLNINLHRCECDYSCQLCDLISGQGCLQCKPGFKLIDRECVTICGDSLITTFEQCDDGNMIFGDGCHQCQFSCESQCLICIMGNCLICAKGYQNFEGRCQELYSQSFPIVKINDEMYQNDLLQAFYYDYQYNNYLNRISLESLEIKSLQSIIQNEIVYFTYLFPNLINSPNQQIFFEAELSLTNYQRNQQNIMFTNCQMNDNKCKNYCIEFTKQNGLKLKKLDNCQNEKLTKIQYLDDSFEQCNGNGFECLKNCPINCQICSQGSCIFCKTGYYLDIIGNSCTPICGDNLVTVEELCDDGNHLIYDGCSLCQFQCQEECIDCQYGQCKQCQENFYFDLLKGRCIDWNNCNEQNGLYIDEQKNQCYTKCGDSIKAESEQCDDGNEIQYDGCFQCQFSCDQYCVVCQQGGCEQCQIGYKKINQECIIDCGDGLILGAEQCDDGNSLNNDGCTNCLIDQGYKCQTLDEKSFCFQCKSECTDCEFKNSQIVCLNCSKGYFISFGECLKCSEHCIECQHSPNNCTECMMENCGRCDNKLGYYNDFSLRKCYTICGDGIMAEMEECDDGNNFNQDGCNQKCQIEKGYECVLNTCNKKEEKQIAYGYSNSSTANCLFLKSEINFENFCTRIKIVIEYFNNTDFNYSLITAQINSTEQSERNTQLHECQIQFDFFKTITEINLIHILIPTSQNQSRLLEEENIREIIVIPRKQVYYNQAQKAQAQAVVSTSNQLTFLLQLIGPLTILFGGVNFFWTILDILTWINNFYFLNVDYPLNVKLFFQKLQWDDIFNIPEFISLNQPTDLYYFEAPPKFNEKNINPLFINNIQVLYILIYLVSLAIIKIFEKKFTHKNATRKGIVVFTICYVESNMQTQEQQQPKQPIHEKLQKLPSLIVLLIKQSYKYKLTFLSKLIAIINLLLLDIFMACILQLICEKKNDYLIVTFNNFLAIFSLILIFGMYQLYEFVSSKHYLLLNHFSFSRKYSSLYEGIDYENKIAKKYCYFNLIRKIAFIVSIVVLYEKPILQTTFCCLSCFINQAFLLYQNPFDSKIGLIQAAVPDFCIFCIIXIIDWSIDNFIWRSKLFLDNFGHINMDQQFLFSQCRLPSKCEIIFLKIIMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W693</accession>
    <name>A0A8S1W693_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0760140</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000076">
        <property type="protein sequence ID" value="CAD8181436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W693"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8181436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="224" mass="26606" checksum="A25194CF16EA585E" modified="2022-10-12" version="1">MNINIYDEASLSLLSTSNFHEKHTISSLNKQKYIKQKYFSESELISQNEIQSHRVRPNSDMYQSGINFLKHKQEKQDYLSLKLEEEFKKKYTFQPQLSQTSRLLSARLNVSQVNRSTRNMNKEGTLNIDSQTMKLEQELIECTFSPKILKTTQDIFDRRKPMYERNVQWKDQVKDKQTQDQIRRESLIKSKIIPTQTPKQMDLRAAHALKKNFSMKYLRKSMMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W6N6</accession>
    <name>A0A8S1W6N6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0810059</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000081">
        <property type="protein sequence ID" value="CAD8183559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="283575at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002895">
        <property type="entry name" value="Paramecium_SA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01508">
        <property type="entry name" value="Paramecium_SA"/>
        <property type="match status" value="26"/>
    </dbReference>
    <dbReference type="SMART" id="SM00639">
        <property type="entry name" value="PSA"/>
        <property type="match status" value="41"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035923658" evidence="1">
        <location>
            <begin position="18"/>
            <end position="3871"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8183559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="3871" mass="429680" checksum="787A516825EFCEF2" modified="2022-10-12" version="1">MKPFLLVLLLASSGLIGKEIYVGSDYECQCLQMMVQDDCLKQGCQWQNGECRVKLRQGAIHEGESESFCSQFDGKNCSSQSGCAFLVDKCVEFSACSSYLQSTNELCQSISKKCITDGEKCVEISVCSDYKTAISCREDTEKNYCYWENGSCSKSLDCGQLPKTLKNDGECRSVNSDCTVSSQGGCQLSGLNCQNQEGEQQCVWDRFMQVRCNWNGISCVDRSCQSAPISIKNNEECNSYLKGCILTVGGGCVELRNCIDITTSEACVVDKNGKQCYWKNEECVERVCSNASELIRTFEECQIFLNGCVPSLKGGCQTFNKCEDFIDKTSCQSGLLNCFWNTKSCVQYSCNNAGKNYNTHQKCSEYKADCTVNVSKDGCQDRTCDNAPKQLKSNYDCELYKSGCITKFNGGCVKNQECSNITIKEACLLDQQGRECFWYLNSCKLKTCNNAPNVYNSHEQCQTYSKLCTVKKTLNGCVDMSCEAIFQKDNCIVDKLGNNCNWIGLCFDKTCENAPVNNNFVTDAQCKAYLSKCTVRNTGMGCMDRPYQCSEMKIPEQCFTNAYNVQCEWFENSCKKKECYTAPNSLTTFEECNTYLIGCTTTGNGCQTLNTCDQYQNSNSCRFDINNDICQWNNGICANKSCQTANNTYTTNDLCNTYLSGCIVNGAGAGCIPKPSSCSEMTTATQCTATSTNSSGGPCAWIGSCVDRTCSNAPASTDYDTFTKCNNFLNTCTVVATGTGGCMTMLTSCSSYTSQRQCEQIVNGNKCSWYLTSCLDRQCIYSPDTSSYDDKNECHSYDNRCNVVRRIGGNGCTIRKATCNELSQHQCVKDSAGTICTWNTTADPPYCLNRTCSLTIGITNFTPTNCANWLSTCVVNNVSVPTGCLALQLSCSSYLYSDNCTYTSSNVQCYWNGSSCVNRTCTTTEGITDFDHSSCYTWMNSCTVNKPTTCADKPTNCSDAINYDQCYKNISDQLCAWVGTSCVDRTCNNHGYAVTEFNDINCSGWMPGCSGNPDQTACEITRTCTNHGSNVTTFTHNDCSNWSPLCTVNSTNDACIERTCKNYESNVTDFTDSNCSNWLFKCGSNDDHTACISSRTCSNHGSAITVFNHNNCNAWWDECTVSGTSCTQKTCYNHSITVFNHNNCNDWYYQCTVSVDQLSCQPKTCANAKLVSYTSNNCYQWLNTCTANSNNTACISKRTCSLTDLTTFNYQNCVNWFNECTYTNAGQCNDKTCTNHSSNVTVYSHINCEAWLQRCTFVSGSQCSDKNCYNYNDFIYIQDHISCNNWLGICTSNGTTCEPKTCTNHGSNVTVFSNSNCKQWLSYCQVNVSGTACENMRGCSPSLSTYTHITCESYNYLCTNNGSGACKLKSCLDTQLINYTHEFCQGYLYECTVKSQFNGCEQKTCYNHGIQVSQLSHANCETWLSNCTYTGSYKTCIPKSCTNHGSNVTVFNNTNCQTWWSACVANSFGTACVNIRTCSNTSLTTYTHQSCENWLSTCTYSNTTSCQDKTCTLASTNSNLIAFDPISCEIWMNTCTSNVAQSACINKTCTNHGDQVTTFNNANCSAWLSYCVANSTNSGCIENKTCSNAVVSSYNNTNCNTWLSTCTVNSTYDGCIDKTCYNYGQSISSFTHTNCNNWLSSCTNLSNTGCQDKTCSNITPATYTSVSCYTWLSYCTGNDTQTACVSTKTCTNYGTNIKILNHTNCNNWLSSCTINSAGTSCEEKSCSNASPTLLSFGPYNDSTCNTWLSTCTVNSTFNGCITRTCSNIPSYITKNTSNCQSWSSLCTYNSSSQLCEDRTCTNHGVFTLNASNCSSWLNSCSFYSNSSDCESIRTCSNFVGSVSHSNCEQWLSTCTRKTDNSGCTYKLCEMYTTIIGTPYSQSDCETYLQSCKYDSSSGICIEKNCTNFSGTINQSNCEAWSTRCTINTSNNGCTFKTSSNAITSLIYTDSNCKKWSEAWSLKSTNDQCISSCYNNGQHFSVFNHANCVAWDPNCTVNSNNTNCTPKTCDNFNGTINFSNCQAWLSTCTSMHDNSKCMEKTCNNQNQNISACFGDSGCQGYKSDCYQQSCYSCRIKTCYDFADTTLTNCQSRYSHCLLGQSGCITHRTCSNHGSNVTIFTHINCENWLSSCTVNDSGTGCTEKTCYNSSITTELKNAYSSPSQITDVQCVSWNYLCTNNSTTGCKNRQCSDSGAWSSCSSYLNSCKSVSSFNYCDYKTCGSATSNSSVTQCKNWLSHCTRSQSQRACHSQRTCSDYGDNIKIFNHVNCEQWNPLCTINGTNDGCIYKTCFNHGMTSTNNTKCGQWLTICKANASLNGCELKTCINYGSAITGSFSSYCSQWLGNQCTHNTVTSAACIPQRTCLKSYNGYNIKYDQYYCLDWLTTCTPNQSQTGCIDKTCTNHGSEVTTFTHSNCYNWYDICTNDGATACKYKTCQNHGSNVSSLTHSNCQSWLTSCTFQTGESTCNYKTCTNYQSMGLSQNNCYNWNNQCTYITYDCEYKTCNNYSGTINESNCRNWLPYCTPNYSGTKCISTRNCSNYGNQITEFNLQTCQNWLPYCTVNSTDDGCMDKTCDNYGTQLSTFTKITCSGWLPYCTNNSTTKCEMKHCTNYPSGFTVNSSNCSSWFSYCKLSPTSTNCEYKTCKNHSLSTFNHTNCSNWLHYCTVSDDGTSCVTSRTCTNHGSQVTVFNHSNCQFWLSTCTINSGLTACEPKTCTNHGSFVTTFNHNNCQDWNFDCTVNVGNTACTAKTCANATGISFNHTNCQNWLSICTVNSGNTACIDKTCSNTTGISTWSHNNCEKWLPQCQLNKPTTCTTLQSNCSNAGVSYNQCVIDSGNYECAWYNGTCLRRTCSNYTGTTFTHTDCENWLETCTIESGASPNNCVNKPTNCTDTGVTEDQCVTNISLVNCVWLSGCENRTCANYTGVTFTHSDCETWLSTCTVDALNVHAGCVIKPQKCSMISNQDQCIKSLDNTICFWTGTQCKDRVCEDATQNTTFDDDTECKTFLSTCTVARIGECITKATNCSDYLQESHCFKNTSQGICFWNVDINKCADIKCSNAPTSYTTHTQCNEFLITCTAKQGGGCMTLNGCLNYSAELSCVIGSNGDVCAWSGGRCYVKSCYTAAYDSTRDTHVECQDYLSDCTVIDSGIGGCVPLQDCATYISERQCVINNMYLPCGWDGIRCMNKSCQTAPKSYTQHEECQSYLNECTTVAIGNGCQFKNTYCEQYQTEKQCIITISNQSCYWNTKTNSCEIRSCQNAPDTSITAELCEQHYQFCYTDYVYCRLQKCSDLIYKTNDECKKYNSNCTSNGKTCIDRKLCSDVHVQESCNTDIHGNECQWNFRFSYCVNKSCNTSPEYNITERDCQEYYPKGNCTTRLGGGCIQKSTCENAQLQAACTTSADNKLCIWDSYRCREQICDDILGTSEQECAIYDCAFQNSSIFGIRCTTKKKCSEFVTEDICLKGIDGICKWLNNNCYLYTGCNTISSQYDQICKSLSKNCTTDGNKCVGLSKCADYSIQQSCITGLDGDCIWWESLGQCKRFLACYDLPYLSHQECYQTSQKCTTDTLNGCIPLLLCSSYKYKEQCRISSNGKVIKQNSNQKYITQTGICIWDTILNECKDQSCEQLFGETHEACNAQLYGCTSDSNRCVTIKECEDYDNEQTCLNAQSSNGKCVYQKKQCKTISCNDIPYGYTHDTCLKQLPNCISDGQLCVAIDVCANYENKFSCNYGGLDGICAWDGQQCLKVLECSDSDLDKYICQKLSEKCKWIENPQITSDTSCFPLECQDLQERECHNIYNIDDTQIKLCTSKDGKCIETEPDTYNQQSCRINTLNTFYWNTTTQKCTQCSNSINIETDIKTNNGAMLMIILIIILF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W901</accession>
    <name>A0A8S1W901_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0840050</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8184875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="297383at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035806644" description="EGF-like domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="2643"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2597"/>
            <end position="2619"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="462"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="648"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="866"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="905"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="2643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8184875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2643" mass="302100" checksum="2F385D0779CD9074" modified="2022-10-12" version="1" fragment="single">MNQQGTNLFILIVFGLLKLCLSDCGYLKTGSTLLSLTDPQQQLDINLDNIVLLGTSNIGYGLWIKYQPFVPISQITTSSSSTGGIIFREPVESGFFIYMQQQKDTKFNMLTVYITITPATNTIMHNIFYSFQSTTGTISFNFEFDEYEGRWILFYVYYDLLQKQTTVGFYQAEQTVSTKTQQLQDIPSLVKYVRHIIGGVQEIKNQLGEILSLQQFKGRISTLFSKEQLNIFLDLNSFLSTCEIVPSCSIKTYKLSGYNQVFTGNGFTNNNTSQLENPSYVIQGWVKMEFLSDDYYLETVILRISINSNYNDDMTIGDKELYLKYYQSIVPTQNGFSITTYTYNFPMEIKYQSSGTDIITVTGPQYQNLLIDWHYIQYEIGTNNNNGQPLFSIYFPSLDKVFQYKWTVPINHFTGTILYYFVGGDNFIKNYLIGQLSDLTLITYCTTPTIMLNPKCHYSCNTCDGPGISNCLSCPINSFRVQSITQKTCTCKNKYVDIENDPICQPVVVKFPQLEEDEVQVVCNKRGFDSCDNNIQKCLFGHFLYRDNCIQCPGYQLYSTRYQILCSNCFFDSVSFAQNLQCLEDTNTYLYDEDNSYFTTQRQNREIEFYIANQNLEGQYELTLQYGFVQSSKCKQGFFLDSNKQCSACLDGCETCVKKGICQKCFSNYALTDDYLCQQCLSCTDCTFYNKNTHCRSCKTGTYLNSEGLCQSCGQYCASCNYQGQCNYCDNPITYFLSFDGKNCEICTIPNCQICYHYFIKNDVTHTKFDINTSIIDMAESQLFIGCALCQQNYYYNLITLQCEIAPQTSGNGSTNPSDPAPPNDQDGKINEVSTELCTFGLIIDSLGTSHCLISQYSVTSTQNIFCSTITNCQQCISNYLSASTFCIVCVDGYYSSILTGRCIQCEQNCKTCIQQNKEYQDFWKWGIKAYYKYVFNQDDSHPFEQYATSTFESDFELICTSCPKGYILYEFTCIKDCNEDCTECNIINGISICTQCLETPYGYLKSQDSKGICLTCPNYCAACLERSTEEIQNINPYFIITQNNIKYTRICYEEFQQDTTEGSYYNDQNIQTITFCEKYDKCYHKAILRQNVYCQHGLYMFDLEKSTDQQVFLQNNIFIDVLFAIGYLRIVETSSLYTYFNEKVIREVEFQFRFISQNGLSCYLQQDSKLLNIIQQNVFSIQQVNLKLIGDKYPTTIIIDKILQIKNFGSITFENIQFQTAIQDNNNSLQSYIVLINTQLKMNLIIKDCIFTTTNSTQQLKQFQFISDQGYSLNIENLIIRDFKVQDSQVFQFQISSGFEQKVIISRLQIITSYLKNTNLFNFQAKSNDLFANIQMNQISITDTQFDKSNLTYTQTQLEYDIGTVIVNNLSLIAVTLNDKSTIFSIQGASSFITKTVTVIDLILQNNSYFYFSNIIQISDLQFNNTMIQNSTLITNDVDYANSDESMLNAAKISIINCKIENLKYTNAQSILRIILYEKINTQMFYIDSFTLNNSISLLNEIPNYISYEQSNIYIECYQCKLISVYIQRGYGLPEITILHSQNLNIKNLYLSQSSNYYSKTLHQSFECVNNYAYSQMFHFLYIGFYQNITIKNLQLSNSITYNNPFIIIKGYDLMEKIQNEQIDIIDSIFDSNMLIITKANRATSIISILSEQSSFVTFSNIKFYYNHLNQYFQDLSRQSTSTLLIQLQQGNFYLENSYFKQNLVTNSTDSILYLKAITIGITNTKFQKNNILQLSIIGYNILLSDDNNDIDGQSLINVLPISSKSGNGLLIATYLNLDRVRIDQSFSNYGGGFYINTQGISSIIIKNSKFLNTQTSMISSSFSTGGCIYIDAQLSKLTLIIQSTTFDSSFSRLEGGGIYIIPSQQENNIEMSNLNVIDCYSIKSSFLSFQSSSLDNIKSQIYISGIIFENTQKGFNTFISLLQSPSISDLDSIRNSNALISIKYASVNVNNCSFSSTQFSYFLNIESAINIYLQNIEIINSTILYSPLLKLSLRQYYYGILMIKNLTIQNVIQKLQIVDIPCLVTSFIIKEPLTCPLIFPSQSKNLSDYDTSQNQQLQMECNKYLIYQNVTYKFSLIEIDDLKVSHHFQAENLQFDSIQCSDCQYGLITIEEIEKSEVQNILFSNVIIRDSNCGQTGCISLLQYESNAILRSDLLSSPTNQRVLQQHDYYNLTYNLNYQAKIINSRFINNNAIYGGSIFIVEITSILHACYFRNNTASIGGAIYYFSKQAQLYIFDSQILENKAKIAGGLYLNSQSLQTTTQLDVYLSNNNSTLFGSDVLENPRSLTMSVDGGKTFLTKKLISKTSTSLVEQIVITPYKILGYSQKVNFIMFPSGRAIGSYKYFDQYTSQYIPYNLTFRIIALNKYNTQEKKLEGSSCTITPTIMNYTSQEILSGLVGSLSYTTVKFNQTSGDFNLDDLIIYFNPTYEAEVIMQLSIFCNIISIPQYESIPPYSINNTITNYKLLIDIKTLKCQLGEYQNETTGGCILCDTIQNQYQVKWSAQNCNYKDDQTMKSIESSMIELRSGYWRAYYYSQTIEYCYHLIQNCQGGWKPGDQSCILGHIGALCEQCDLYNIIGQGSYSVSSKYSCGNCDQIIGNVLTIFFISVWTLISILMSVSSTVEMIEEFIAGLRLKAFGFTXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W9M6</accession>
    <name>A0A8S1W9M6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tyrosine-protein phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0860169</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000086">
        <property type="protein sequence ID" value="CAD8186121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W9M6"/>
    <dbReference type="OrthoDB" id="10252009at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46653">
        <property type="entry name" value="SPECIFICITY PROTEIN PHOSPHATASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46653:SF1">
        <property type="entry name" value="SPECIFICITY PROTEIN PHOSPHATASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8186121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="300" mass="35157" checksum="1C8153D9AFBCB8B1" modified="2022-10-12" version="1">MQTPTRIIDGLFIGDQSAAHDLEFLITNKITKVINCAGKQIPNHWESIGIEYLTYGWIESDNQYAIDHSRCFSFINDNLEAGEAVLVHSIKALNRSVFVIAVFLMRKFKWTLQKTLQYVQNLKNNNDIKQNVMQQLVNFEKWLHISKLSNNWDSTQNQDEVVARNTYLNSQKPQECEPRQKKEVGSKKVQWNKIIISQYIPPYFQIQFKNLSKSEPEPLNQVQNQNIQLTKKLRRQTTDNWMTKKLPLNQVIDEKKINLFSIKSDRQQIKPLLRPGTAPQKRLVKNKWQSQSHRVLVKQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WA74</accession>
    <name>A0A8S1WA74_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein mS35 mitochondrial conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0850013</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WA74"/>
    <dbReference type="OrthoDB" id="283424at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019349">
        <property type="entry name" value="Ribosomal_mS35_mit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039848">
        <property type="entry name" value="Ribosomal_mS35_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13490">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13490:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10213">
        <property type="entry name" value="MRP-S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small ribosomal subunit protein mS35 mitochondrial conserved" evidence="1">
        <location>
            <begin position="44"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8185295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="120" mass="14187" checksum="8BE268BA63F33DB4" modified="2022-10-12" version="1">MKQTFRDPFDYPLRTKKEIDEQLKSNPPTYQVFAEISFIPDLKGDNLHKNSEISVYVNISEMNLTPKQRDRFIYLIGPRYKGKDEIKIKVNTFNNPLQNIEKGKEMIYELFLEAIRAPCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WCY1</accession>
    <name>A0A8S1WCY1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphofructokinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0880032</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8186881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WCY1"/>
    <dbReference type="OrthoDB" id="537915at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050929">
        <property type="entry name" value="PFKA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005301">
        <property type="entry name" value="PRK06830.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45770">
        <property type="entry name" value="ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphofructokinase" evidence="2">
        <location>
            <begin position="181"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8186881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="546" mass="60500" checksum="4831D3A9810930B7" modified="2022-10-12" version="1">MESKNLTQNINPLYLLASLALGTIGAAAFNALRKGSTETAQQHVFEENMSANASAPQSPKRPTKKHVINKLMAKNANENELVKELKNLEEITCEIERVSNLAYDLKLQVLPLNVNPMLGNSNYRSVFGGEGFLPETAFVNHDCYILQHPNFGPNDLLSRTKRWLRSGPRKHLLFSPDEVKAGIVVVGGLLPGLNVIIRELTMCLFYNYKVQNIFGAKYGYNGIYNNDWIKLDPKMVKTIHHQGGCFLGTARSKFDGEMIVDELAKNGINQVYLIGGLGCMKSAEQLYEIIKDRNLKISIVVIPKSIENEIPIIDKSFGFETAIEEAQNPLKAAYQETHSKRYSVGIVRLWGVHTGFLALNASLAFRSVNICLVPEFEFDLYGEKGLLQYVYSRLQHKGTCIIVVAEGAASSLRDCKINDKGRDASGNVKQGDIGLFLKNAILEFTREKGLNAEVKYIDPQYMVRAGKANSLDSKLCSQLAQNAIHASMAGFTGFAVGHVSNKTCMIPLNEMNSGNYANRITPSNISWQRLLAGTGQPSFINNEQDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WG15</accession>
    <name>A0A8S1WG15_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0930070</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000093">
        <property type="protein sequence ID" value="CAD8189174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WG15"/>
    <dbReference type="OrthoDB" id="309416at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="56"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="343"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="75"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="156"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="232"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8189174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="782" mass="90882" checksum="CE8D2077262463F7" modified="2022-10-12" version="1">MSVNVIPVANPHQKMEGIQNVRPEEIKKIIEKAKNLPSKQEGDFVKLQNYEFQVDEFEEMEDPQKNNKKNKKQPKKDSKKEVQESKDKEEPDQSSDIDLKKQQKPKAKEIKKQEKPKPKQQQEEDIQDGQDNNQEVQLQDEQQQQQQQQQQQDEEVQLKESEIDQDDMQKQLDEYDEQNNQLSQSQAQDNSQIQQSQISQKSSNQKKKKFQSGYAAMMDDLLNESEVSINEEQPKNQKKEENQMVSSQIDAKKEQEYLKIIKQYQSKIKQLNEAIEQQKQPVLSEIIEDQIEDIQEQLDKTEESFSNKKHLYPGIAGIGISLYDEFRLPQIIEEQVQHRLKELGVQDRQNEENEQKIKDLEEQHQAELNRIKQNYEEQLQQMKLEIELQQERIKEAERMRYAESEISKVIDKDEIELLRNDPLFSVMSSNLVQSVDSNVSQLMKDESIYLKYRDVLQQYKPKANQLENCMEIVNSKIHDLITLKEAQELNQFNTDSNISISKIMDIDGAIRIIDQNQNVNSDIDSLITPSEQMKFKKDPKYQEPSLPNSSIDFSIPDNYKLQRMIDSDYSNLDMKINEDGSLFIELQKELEQNYPMTESNRLIVYQQIIQDQKQEFDKQVKKLQKLKPTYKSKLYFGESIDSSIGSLANEQVPKSISSNISMISKSQSNESIIGSVVSKNEKAELQKQCYSKESSISRLQLSKNDVEQLDKISSGISQLNSIVDSYNTHNQSVSSGISKVLNDQQLQKEKYSNIDSSLNISQGGSSIKSFLLLKKSVISKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WJD1</accession>
    <name>A0A8S1WJD1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sperm-tail PG-rich repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0920120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000092">
        <property type="protein sequence ID" value="CAD8188845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WJD1"/>
    <dbReference type="OrthoDB" id="406368at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051291">
        <property type="entry name" value="CIMAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580">
        <property type="entry name" value="SHIPPO-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580:SF60">
        <property type="entry name" value="SPERM-TAIL PG-RICH REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="407"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="489"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8188845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="580" mass="65806" checksum="311053DF654223AB" modified="2022-10-12" version="1">MAFVFQAERDINNASGALPPNVGPGSYIEHRKYEARSHAAPFNTQVARSKPLKKSNSPGPGSYNVEFVTEGQRVVLQSAQSEVKIVEQQKQQSVFMSQTKRFQEQKVLETPGPGAYESTSQKKIAQQQYTSQNYIDNLMKLNKYQSIPSIPTATQVYGYTDKGPHDLELNKSPNPIFTGLKSDTVGPGQYQLKDTFDNNKNKGPSWHKSRVPKLAPPSREQIVGPGAYDHENSIIPLYKLNPSGNFLSKSQRIFDQQKGQKTREFMRVQFENQKKKLMQNPVFADIEDEDVEFYDNATPGPGYYIGNQTTFSTASTYSQSMPKAQSGFGSKQKRFNEQQRQIQMGPGDYRIETNLIKNNMAMKNIPFLSSNTRFDSRALEKKPGPQSYNPKITLEDKLIKKLERAPVGKFGSNQPRFDDPENEQPGPGTYEPNFSDPAKSAACVFKSQTKRSVIGGKDNMPAPGAYDVKNYTIENSTKVEKEEDKDLIINKPGFGSSLPRFQEKQKKSIDEEDEEEDVQLKHNASDLFQKKKKEHPAFNCQENRFQYEQKKNNLPGPGEYFDQKQNPWDKKTFNILFSEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WK21</accession>
    <name>A0A8S1WK21_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0910167</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000091">
        <property type="protein sequence ID" value="CAD8188489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WK21"/>
    <dbReference type="OrthoDB" id="10435817at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8188489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="198" mass="23631" checksum="F938CEBD96E2AA3D" modified="2022-10-12" version="1">MRFRKIKEFRYKRRQNQQLQYIQIPNNQVPHNQNNNIQNYQRQQPSPQNQIQVNSQYSNSIKLNQPQPYITAPNIPQNVPQLLWPYLCLFVFNLGLGIAIFATQGSETAIIIFEVFKMLLNIISGIYYYSKSTETIFNVPQCFKILFCIILTQFFCNFIQLLICWITQVSYSLQLVTIILLIIFLLYFYYQRKRYVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WL37</accession>
    <name>A0A8S1WL37_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PB1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0970170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000097">
        <property type="protein sequence ID" value="CAD8190894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WL37"/>
    <dbReference type="OrthoDB" id="441278at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05992">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PB1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8190894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="285" mass="33928" checksum="598EB527C7524FC7" modified="2022-10-12" version="1">MIIKLVYNKEIHLFKLENPVILEIKNHIQKLHPQINQNYSLFYRDDEGDLISISSDEDLKVLMESVKNQTVKITIQTSNTWICKTCTLENLEGVVCIACSSYKEGEPQQHGQQYQGFQQPYYQPQCYQAPQQFGPPPPQQPPPQFPFVPHQMPPCPPFGHFGMPGFGHPPHMFPGFHHFKHHRHHHLKWQKFRQIMKDPQFKQQKEKLQQLLKEVSQTLRNTMQNTQQQQFYQYQQQWNWNEMDEQRATRLAEVLQCNQEQAREYLLIFRDLTLDEIVMMVENAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WMT7</accession>
    <name>A0A8S1WMT7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0940190</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WMT7"/>
    <dbReference type="OrthoDB" id="294560at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="48"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="318"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8189765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="349" mass="41707" checksum="4960C29B95182E9F" modified="2022-10-12" version="1">MIRQFKCERLHYILNTNYIVTIYKDKLTIGNESHPKYEYKFKLENVLHWYVDNLKLQGFGIEYKGIIKFFKANYKDLEQLRSFCRNLICFDNIVELYKQIDINNPSRIQDQISLQLCGMKIVTQQQLLTQVPLIRQLNYNGIISYRECFQYKSQYYIITEFVGEITLRKFITQNPKLSHLQCRAIILQLFKTVQYLHDNYVIHATIDINHIIYKSDFIKIIDFSQAKYTNILDDYDIKNCSRILFYMYTGKEYNDIDNEQNLQTLQNTQTPKLAQELILSIMTDKNLTVSQILEHPYFSFSLDPQERKNRFQKFQRIQRSTSEMDDSDAISQSIPELQTNKSKSLRQLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WNV2</accession>
    <name>A0A8S1WNV2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0960054</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000096">
        <property type="protein sequence ID" value="CAD8190311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WNV2"/>
    <dbReference type="OrthoDB" id="10345896at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="125"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="40"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8190311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="161" mass="19280" checksum="978A780FA79DA3A4" modified="2022-10-12" version="1">MLNKSIKVTKKVIIINQKTDTISDNTNLINREPLKNGVEIQKINQKNSNQIQMNNELDQQKYDSRQLQEMIDIYLKNNQKIKEIKSIVDSLKNRTNKLEQQMRQQEGRCKVKRVRRTSTQIEKKYKCYSKNCEKSYGSMISLNLHMRIKHKRQRNVQEQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-06-18" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WNW9</accession>
    <name>A0A8S1WNW9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0950171</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000095">
        <property type="protein sequence ID" value="CAD8190165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="311329at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050349">
        <property type="entry name" value="WD_LIS1/nudF_dynein_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44129">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25173">
        <property type="entry name" value="Beta-prop_WDR3_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="NACHT" evidence="5">
        <location>
            <begin position="686"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1463"/>
            <end position="1504"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1505"/>
            <end position="1546"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1547"/>
            <end position="1588"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1589"/>
            <end position="1630"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1631"/>
            <end position="1672"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1673"/>
            <end position="1714"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1715"/>
            <end position="1756"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1757"/>
            <end position="1798"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1799"/>
            <end position="1840"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1841"/>
            <end position="1877"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1883"/>
            <end position="1924"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1925"/>
            <end position="1961"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1967"/>
            <end position="2008"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2009"/>
            <end position="2050"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="266"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="522"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8190165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2135" mass="247681" checksum="9E4F4B6A427488F3" modified="2022-10-12" version="1">MNFSLKQLRNILKKLLSRCNIQNNNISKIKSIKISNDDFKNNLSNQKQIIEFLLFKLNIEELINDSERQDLQLIEKHFGELFIEQSSLPLHVEKLDQIIEGLQIDDTINKITNENFNQKQLKLEEDKFQNFLNQLILIRESSQDSNQQIIEIEILIKKAEVVVETFLNCENQNIEIFKIPLCEEICDLQKIIENCKKNKDKQDNEKQNIYHESHLNKLNILNNTSDLDQFVKKDQIDDIYYQKLKKFNNYFDEKYITNLKFIIKIVKLYQIKIKEEKQVLHELLEEIQKFTSKIDFMKKYEGTIYNKMQQSFIACFEKMINNFEQKYWPINSISLQKNENQQIYFERIFANFYSIINEYEKIKNLTDDLKYPALCSQIDQGAIQKLEAKFQGKAREKSFRSQQIQIKQEILIQTVSKIYLIHNLEQEFEKDPNQQIGLFKTLIQKYKDLKNNDEWKIKQGLVFSIISISSNCFTDTIIQFCQKALIQIWIQEKDERIRNLLKNQNLITSQIQILKKDWKTQCDRISGEMQQLLHKIDDLQEQISHEYNLKKRDQQLQEVDETTQQLNEYIENISEMGQQLDLIHNFVSNIRQGLKRIEGKINQIKMQLNNMDNDIKFLRGKSVEQLLEIRKWKVLDEAAYKKIKSIYVPLKTQEKMKENIKNLMSLEEFDNKVGAVDEFLQQEKTVLLIHGMAGSGKTTAAKKIEELIWKLHENNRKINKLQLIPIYILLPSLKNPVFSAVEEALQNDYGFDQLQLKECKEMLEKKQFRFLLIMDSYDEMKLENIQKNLYINNKINQNWTNPLVIFTTRSEILTNSNYAQWFAPDDQEKLKEIQILKFDQVQKQEYLQKYTIQSIKMLIFEIYEWQIQVQNREVIDINKFEMCWEKLQSSILKYDLSMSKSETLLNQKQIESILLYLNDDEFIALKSNEAIRSLRINLQKLWSVEKYDNMMQQMNLNKFMETPYMMEIIVQVLPEMMVKAAEIVNLKQNFIKNFSKMFKDFFKSKSMIHLYNQQKKNYLIKVSNEVEKETCQENFFDQNKKWEVLQVQDCFDSTSTGLQIWEKIDNLQIALEVWNRMEENSIPSQLQISSEFNELSQKLLQIFKLNRGLPINELNKVEIKKENLIQLVFDALKEQNLTNYDFYDEFINMYHLKQIEKQRSLRKSINIDRFLHDLKQYSIKLAKTMSKKLVTQVQYQQQGLLYKDEREEEKWLNEFFNDDGQYGSYKKDIRSCSLIQQKGTNFQFVHKSIQEFLIAADLYEVLVLSKDFDIKIFRSIIEILTKQRYKEQNCEEFLSSQEHFEIYINVDNLSLFERQQKFNTWQKSIMSIINLIKILKQHDFNQINYSTEIYTETRKFLIKKISSEIKIIEFLKLIVHLTSLDKNLIQSGSNSLNILVEIKVDLTNQFFLNIRIQNTSLIGGNFAKCNLSGAEFQNININGMNLNGAQLIDCKWKNLRINDLHQLNGHDGPVNSLCFSPDGIILASGSMDQSIRLWDVGTGQQQSILEGHSNSVQSVCFSPDGSVLASGSDDKTIRLWNFKTGKQILQLNGHHAEVLSVCFSADGSTLASGDGDKTIRLWDIKNGHQKSNLEGHSNNVTSVCFSPDGTNLASASWDNTIRLWDVKSGQQMLKFDGHISYISSICFSPDGTTLASGSNDKSICLWDIKTGQQKSKLQGHSYGVYSICFSLDGTTLASGSDDNSIRLWDVKTGQQKSKLDGHTNAILSVSFSPDGIKLASACWDNSIRLWDVKTEQQKTQLEGHSSFVSSVCFSPDGTTLASGSNDSSIRLWDVKTGQQKSKLDGHSNAVLSVCFSPDGTTLASGSNDKSIRLWDIKTGQSMLKLDGHNFAVYSICFSPDCTMLSSGSDDSSIRLWDVKTGLQKLKLDGHTNAVLSVCFSPDGTILAFGSDDNSISLWDVKTEQLQLKLEGHNGWVQSVCFSPDGRILGSCSGDKSIRLWDVKTGIQKSLLNGHTHHVQSICFSPDGTTLASGSNDCSILLWDVETGKQKSKLDGHTHYVQSVCFSPDGTLLASGSCDQSIRLWEVKKGQIINPLEENYKDNFAKFFKPLQHISFSEDVNNNITIQHISQQPIFQAKGALILNGEFTNHLGMDLRKLFEQRGSFILETYLEQQKNKNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WYQ2</accession>
    <name>A0A8S1WYQ2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1040039</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WYQ2"/>
    <dbReference type="OrthoDB" id="290543at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45641:SF19">
        <property type="entry name" value="NEPHROCYSTIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45641">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_6G03870)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="60"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="61"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="101"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8193345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="456" mass="52901" checksum="FF2929046F98AAC4" modified="2022-10-12" version="1">MEDFEILDFLELQQEQQGIDKTARFLQKQIQNGFKISIVIKKQHKFALAPLQQKKNDLFSKQHQPMDVTSQSSEQTENNDKQGSSTSSHSSVKMNKKLIIPPTQNETPEQNNNEQKSEKSDDDQISLYSKKNCSFEHLLKDNLLSQKIQTGLSFMKDGNQKMALEKMMDAYREGNEKLKQCQDQQQISVLIIICIQSLCYASQLLMDFGQFSESQKCLDKCLNQFEINDFDLKCKIYITKAQCNLLNNQYLQCLDNYNKALIQYEQINWRLEVAKLLVKISFVYALLNDFTDAKKICYEGLQILQSKLSHTDPRIYDTYYTLGCIYYLEKDYDFALEYLNQSKEGFIKLYGEQHLSIIRILNLQGVINHLQGNSIDAMELYEQIVCLYGDSQNIGLALVLNNLALVYLDRMKFKSAKLSFDKATAILKVYLNEQHPTFKRVEKNKKLFVAATLSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WZN5</accession>
    <name>A0A8S1WZN5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0990030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000099">
        <property type="protein sequence ID" value="CAD8191446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WZN5"/>
    <dbReference type="OrthoDB" id="10250320at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20557">
        <property type="entry name" value="CYCLIN_ScPCL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF108">
        <property type="entry name" value="PROTEIN CNPPD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8191446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="158" mass="18604" checksum="4FCB536757409874" modified="2022-10-12" version="1">MKGGAAVQSIVVIDKIQILLNKIFAYSNLAESKGQGICPNIFIRQQDIIENLVILQRMGVNDEIFVMGLFLVDRLTAKNKFYLNRRNIYNLLMTAIILSLKMYDDNQIVQNIYPIIFETNREVLNKMEKKFLKLIQYQLYVKTEDFFNYRARLLKLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X0I7</accession>
    <name>A0A8S1X0I7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1100156</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000110">
        <property type="protein sequence ID" value="CAD8195818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X0I7"/>
    <dbReference type="OrthoDB" id="310788at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8195818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="604" mass="70981" checksum="F78EF541032DFBFB" modified="2022-10-12" version="1">MLTQIKFQVKKSINNEMLYMMGGKIIRIDQQETSQIMTNLEQIEHLTWQGERSHSFQKIGRWTADWNWNYEGVGGYYSENGQKQGQWKEMAQNFGEYLLLEKYKRNAKVYEVGEYENGVRIGTWNYVFENKRFGGGVYKENGVKNSKWIELSDNFSSQSQITYNGEYQDGKKFGVWTTMYRGHGYDEFIQIGGGQFDENQKNGEWIELSNFFSKYYFEFNNIYSLSQVIYQGHYLHGKKSGQWDTMARLSDGLKFAQIGGGFYDKNGKKQGKWVDLCNYFQSYVQILYNGQYQDDLKIGKWDTIFREYSSDKYCEIGGGSFDEKGMKIGNWIELSDGFYRECQIIYQGEYQNGLKVGRWEIMYRKKTNDDYQKIGGGLYDPCGLKDGIWMELENSLSRKTQVSHVGQYKNDKKYGLWEIMFIGEKMGGGIFDENGIKDGQWREISDNFSRDQHIFYQGEYQKGRRIGRWDIEEIVETSYDHEDFTFKYTIGGGLYDDTGLKEGIWLELSPFQYWCQILFTGEYKKGKKIGIWNTSKPNELIFGGLFDETGRKDGKWIELNDGFCIQKGEYQNGQKKGIWDILKSRGLWDKEYEKISETSYMDKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X1U0</accession>
    <name>A0A8S1X1U0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1060184</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X1U0"/>
    <dbReference type="OrthoDB" id="292980at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8194419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="139" mass="17302" checksum="59C4262A0FEA5A06" modified="2022-10-12" version="1">MDQQLEKWKLNNQYFIRDKYFNLYKKCGNQALRQQDQLHFKRQRIFRNTSFKDREKLPLMEQSSKKCDQSLPEFVYPTIQQVNTIDEYNKILQERIRSNERKERNQWKLPPLEREYKEKYQLKRTISQVDLKINLQQII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X3S7</accession>
    <name>A0A8S1X3S7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1130086</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000113">
        <property type="protein sequence ID" value="CAD8196673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X3S7"/>
    <dbReference type="OrthoDB" id="309000at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027353">
        <property type="entry name" value="NET_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45926">
        <property type="entry name" value="OSJNBA0053K19.4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17035">
        <property type="entry name" value="BET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51525">
        <property type="entry name" value="NET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NET" evidence="2">
        <location>
            <begin position="192"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8196673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="295" mass="34270" checksum="A5BED5E2DA98F456" modified="2022-10-12" version="1">MASPIDFNLNQIQVEFILGKMPKGFSLLQASLLKAREQRVSKKVNKYDASFQSNKQEVVEDKTKRIQRLTSIQQKESNKPLMDDNHKKGLQLLQMFKSHPAYTQIDTQGQINIDKIEAFFIQEGNLINLKNQIKQTLQKLTQNSSNQMQDLLNQLEIHFLNVFKSPTNQSKPTPIQNKNTQNKNILQKTKEVLSTKKQDQIITFEEKKQLSQNIRELSSDYLKGVWEIVQQTVQTKEDEELEFDIDVLPPKIIRKLQEYVKNKLKTKKVKIDTSINQSSQRSLNQDSSFASESFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X3U4</accession>
    <name>A0A8S1X3U4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1100058</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000110">
        <property type="protein sequence ID" value="CAD8195657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X3U4"/>
    <dbReference type="OrthoDB" id="298119at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="13"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8195657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="252" mass="29995" checksum="47C6827797746AFA" modified="2022-10-12" version="1">MILYDNNLRKSLINKVVDQLHNAQIEAEKLAEMKIDFNQLNKRLIEFSQEVIELWRKGYSQREYSLYKERLQQLIGMHKNSLSKISDQSRSKNTIKKLSKQSSENECSIQTKVKFQHDQALIPQQIQILKEDDFSDISFASLTSSNSETIQEIIEHSEKNTQGFCQVHNCRELKIIREIYRQKIDINELQLALEEIISLKSQIRMKLQKENQKIQVMHERLIEGQNKLKRMEEFEKEQLEMIKQKRMSLNLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X546</accession>
    <name>A0A8S1X546_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycoside hydrolase family 5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1100101</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000110">
        <property type="protein sequence ID" value="CAD8195719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X546"/>
    <dbReference type="OrthoDB" id="1887033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041036">
        <property type="entry name" value="GH5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052066">
        <property type="entry name" value="Glycosphingolipid_Hydrolases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31308">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31308:SF3">
        <property type="entry name" value="ENDOGLYCOCERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18564">
        <property type="entry name" value="Glyco_hydro_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035770968" description="Glycoside hydrolase family 5 domain-containing protein" evidence="3">
        <location>
            <begin position="17"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="4">
        <location>
            <begin position="67"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5 C-terminal" evidence="5">
        <location>
            <begin position="416"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF18564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8195719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="511" mass="59576" checksum="9BF319DB2ACF3795" modified="2022-10-12" version="1">MKYCILLFYLVFVASSFKVNRNDQFIVDSDGLRRVFHGVNVVYKLPPYYPPVSEGFDSNDSLSDFDLDNLVSWGLSFIRLHVAWEGVEPVRGQYNYTYLEEIDKIVKKCEAKGVTVLLDSHQDVLNRFFCGNGFPDWTIARTKQKEFRFPYPYIFSKIHTDDEGVPLIEDCLKKKFVLYYLTAEASKTFQNLFDNKEGIADAFAEYWGVVANYFKDHKNVIGYEIINEPIQGSIYHSIREFLFPNYGNNHNLLPLYRKVHQQIRKYDNEKIIFFEPGTTDLIGAGFKDTPGGEEYRDREVYSYHIYCFNVDDYSIPKNEQVCNYMDSFFFATKEGVAKNLGVGKFMTEFGAILDTPEGNKELNYLLDLMESRFTSWAYWEFKYYADVTTQAHPGQIESFYDEFGQLYTNKVKTLSRPYVTKICGVPSSSNFNRVDDDSYFLQLDWRASDKCNGKQTEVFLSREFYFDEVHYHFTKCSGCTLKAISKSYYVVELPEDFKEGTTISLIVTNHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X6H4</accession>
    <name>A0A8S1X6H4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1120130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000112">
        <property type="protein sequence ID" value="CAD8196442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X6H4"/>
    <dbReference type="OrthoDB" id="296516at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="53"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8196442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="84" mass="9868" checksum="60E934540C2D91B2" modified="2022-10-12" version="1">MATTQLELLQQLYDLRNEFNEQLKKVQAAPENQAEIVTLQDQNRRYAYRIGHLTKAIDELIQSENQLRTENAQLKARIAELEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X8R5</accession>
    <name>A0A8S1X8R5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RCC1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1150075</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000115">
        <property type="protein sequence ID" value="CAD8197376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X8R5"/>
    <dbReference type="OrthoDB" id="10256179at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051210">
        <property type="entry name" value="Ub_ligase/GEF_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870:SF466">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="RCC1-like" evidence="3">
        <location>
            <begin position="72"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="98"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="198"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="246"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8197376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="339" mass="38627" checksum="80D7056DAFF0014D" modified="2022-10-12" version="1">MQQQIQYYSCGSGGCGLLLQETLEDLNIPKKCQQLINKNQNDLKIKFSGLIGLIKENDKIFIYGQWISLYTIPKEINQIQSVSKFSCGWQHCLILTDKGLFGIGSNKFNELGLELKQIYEEPQQIAFDHTKINNITCGFRQSFIIQDELIYGVGQNKQNELNILNIKIVEQFTKINIQGSIRNIKCGQKFTLALLNDNSLYGWGSNSFGQLSQESQFQILEQKELMKDILDFDCGWSHHVILTKNGQVYICGRGDLGQQGDNKKQHNYNYQLIDLKVTKVKCGSESTILLNQEKQEIFVSGWNEHGNLGLGHNNNVYQFERIEMINSIKAKGATILLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XAL0</accession>
    <name>A0A8S1XAL0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1180031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000118">
        <property type="protein sequence ID" value="CAD8198246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XAL0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="8"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="264"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="446"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="526"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8198246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="771" mass="91434" checksum="9CB4BD67EB8C88E1" modified="2022-10-12" version="1">MDQQSPDIKELLLLYNNAIQIKKRAEKKLIRFTNCLSELDRRNCGVEIIEQINVVRQQIKEERKNQAEQTVRNSYVQMQNQMDKSSLILKQKLQEKELQLESMELEYKCQIQQQQQIINHLNIQIEEVKDYNQEQIFCLTQKNQQLLNLIQDLNNQIAELKQILEHYQNEEFKYQNNNNSSIRVSQNSQMLQNIKKLGSQDYNHTIESMDQCQFETLASEVKYQETCENFDEEILLGSYNQCSESKDLQSPIKIQDQKINPNQINELTFTIEELKEQLQNLQFEKQRLIDNLKENQENFDSIILNTQQNQNNLNNQINDLIQSNQELFTKEQILQTQVQEQINQIEILTGQLVTSKQLLNKNDETISKFKQEQEITKSQILLFKEQLEIIPKLEQEIKHHQLIIQDYENQILSSTVKRSELDDKLNQQHINQEKFKTCELDLTKLIQDLNSQLTEFQIKYQVLEQENIKQQQQIILLNEQKNKQQLQFEDLKQKYCQIQQDKIEQGFYKEKEEDVTKQSEQNKIQNYNLQSEINQLKIDKEELIKQNNSKDLLIQLQQQLENQYKVLEEQHKNAKKQYQDECNKITEYYKQEIKLMQEQLIRNSENIKQYSPVNDISQDDKYKEYINRLNDELRQSKLQIAQSSTINNQLKQQQKDLVLKLQSMLKLTPAVDQDLNDLILIIESNLSSCQSSPVRQSFKRTSMIDTTKMLMNKSRLSFGSNRGSVQVSQKQLSDNGYRNSLNIQKVPQSSQSNTDLQKILDDVSDQDIIQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XCJ5</accession>
    <name>A0A8S1XCJ5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1190126</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000119">
        <property type="protein sequence ID" value="CAD8198688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XCJ5"/>
    <dbReference type="OrthoDB" id="293135at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8198688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="132" mass="15638" checksum="457441A9F42B7B22" modified="2022-10-12" version="1">MDEFKSNSERPQTSHILNQLKFLRRNLVKQPQKPHKSTRSHFVPADINNMKYVQSPKQIFGNISSSLTSFKGKTISQSQIQYDESYLNRLLPKETIRNLNNQTKKTNTFFPLFQQPQGRMKTDWINSKYRLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XF62</accession>
    <name>A0A8S1XF62_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1220017</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000122">
        <property type="protein sequence ID" value="CAD8199538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XF62"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000085">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="224"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8199538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="538" mass="61047" checksum="0A537DC0A1B91369" modified="2022-10-12" version="1">MNPIRVYPLKFTNQDSIANSPGLKKKAQPSDRTYTFQLNPPSQQSRSNQKRHPTGGMNIQDEKIKIVTQKKLQLLQPSSYSPKKRVISNNNDKPYIFKFSNQSNEPLSPTKLPNLKRSESTNAPQELQARIDLTQSQRRSKRTDASNTDNTPQNKTSFLKLYQDSSPKLRSIQSRVSSQAIVQDIKIKPKMPQQMIMLHGRSTSQAALVNSNATEDDELQDFVQLFLSKTQAGQNGNKQTKTNQDSIIISNNFGGIKNRYLFSVCDGHGVYGHYVSQLIKRVLPTIIDQQLKIFIGKQEIDIGEDLYSEVEKCFQSSYQKMTKDLSSCGIDIAFSGSTCSTVFVSGNNLWCANIGDSRSIFIEQNGDCNKWKIVELSNDHKPDLPIEKKRILASKGRVQPFVAENGQNIGPARVWLMHEQIPGLAMSRSFGDYVASTVGVISDPELIYHKMTSKCGFLVVASDGVWEFLSNDEIQHIICSYWSPQMNAKKIDEMVEKDGKKRMMWLMIFQQLLHISLDYFNMLKSENLRISLDYFLID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XLU6</accession>
    <name>A0A8S1XLU6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1290077</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000129">
        <property type="protein sequence ID" value="CAD8201889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XLU6"/>
    <dbReference type="OrthoDB" id="20872at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0085020">
        <property type="term" value="P:protein K6-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF8">
        <property type="entry name" value="BRCA1-ASSOCIATED RING DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="782"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="839"/>
            <end position="862"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8201889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="923" mass="109431" checksum="D99431A877EE893C" modified="2022-10-12" version="1">MDDDIFLGLETIQQFDEKMKQFSQQHQFRRQLQRYPVQKILKRSNSFAQSQISTAINSSAHASRKQTISQLEMNPPKRRHCEFYNDEFCMNLFAEKIEYLCTQCYDKNEVVIKELNRVAQELGISLHRVQQFESVQEREQYILSQLQNQVLDSIKQWKQRIDQREIQRRKSDNITINTNSQDKMLDIFDWIEIDTKFRQLKKRLKFALVEMINDKNNTSKISFQLSNRTGDTIQDLLYKKPDTRQIIDELTNLLKTIKNKRNVSNVQLNSPKSVRIEKWNQSKEEQFIAKRLSAKQRLSKLINSSSWNNIAIGEFVSNLDKGQQNKQKRQELESKFESYQSMNEDDLLYLINTSVLLNRCSDKKFIQSAINHLDQDIISDIELQIAENILKSHFDIQKQVNQTRHDFLKERNEIQFIKNRQAKNILERQEKQNSIKKLQIKMCNILREMKDKTKTTMKQDSEKYNLYYQEYKQGNPIANPYVWKFKEIMMNVIKKKKNERRDQKLGVKSENHKPIFPSKEESLILMNVIRKTHPSSKLRLIPQQQQEYFPTPAKVFNEYKLKVQKKEYEKNQSNFLNFYTGGPKKPRKYASPQLQFIEGGENKSDWITPEIELIAINKIKRALKAYVSRKRYIREREKKRQKEYQEKEKDPKFFRILSRMKSKNYNSLDIEECYKSLKQAIAQSAGNSKQLLQKGVQMDYSKVKVPHSMIQLKLKQRKLFLALKVGNTNWAEYSGFQFEPQDVNCYDTNMYCPLYHAAQLQSITFCNFLINAGADVNMPCQGGLTPTFAAFITNNIVLINLFISNGANIDILNDEGKNPLCYCSIQMLKELNLQYNPVNSKSQTQRQKSRRTQSNLEQNYSPYAEMKMEPMLNVNDEIKWQQDQIVSLRTFYTKKERENFYYLKQKAQSQKESENNLENQEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XPA4</accession>
    <name>A0A8S1XPA4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Homeodomain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1300151</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000130">
        <property type="protein sequence ID" value="CAD8202302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XPA4"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF69">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_38, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="4">
        <location>
            <begin position="70"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="3">
        <location>
            <begin position="73"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="75"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="122"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="4">
        <location>
            <begin position="125"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="173"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="4">
        <location>
            <begin position="180"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8202302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="360" mass="43429" checksum="AF7FE90600D99E11" modified="2022-10-12" version="1">MDRYEGAKSLMHSQSTVEQTFERKDKSLHHKDNKIQIIKAESQNKKISLNQTEMISLKQKTMQKRKKICINKKIRKAWTAQEDELLKRSIQQYGSNWVRIAAQMVNRNPSQCTQRWKRIKPQSLKKRKPFSEEEDQLILQLVNKYKQNWGKIAQQLPERTNKQIRERYINKLNPQNKFEPFSEKEDQIILEAYQEMGSKWTKIQNLLVGRPENMIKNRFYSYLRQKFLNIKNPYYSIPSKTEITSLDSKSKMEKDEQMTEEKQMKEIEFQKIKIEYSNQRNNDIKEIQTQLPLEPYPNSQIPQNQFLGNFSYPQFYIYQTPNSIVYTPFQYGHINYQNNLFQNQIYMVQQENNQFQYLSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XPR5</accession>
    <name>A0A8S1XPR5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1330088</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000133">
        <property type="protein sequence ID" value="CAD8203219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XPR5"/>
    <dbReference type="OrthoDB" id="295298at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8203219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="190" mass="22056" checksum="B5CA69ED47E97FA1" modified="2022-10-12" version="1">MSRDPQLELLNIESTLREFIQMPLTNKLYHQIETDFTAEPWDVPITPAKSSQQKKRIITFKQPITRLLPRQSITETPTNISRPKSCHHKSAFVELGIPTPQPSYPKVLQRIIISAQHKSSRKNSTSQYRQKCLKNILLAQKQNSQQCNYTHTSTDYFNFISSINPVSQKNKTTTEPFLKKFKLKLNNMLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XVC4</accession>
    <name>A0A8S1XVC4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1390093</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000139">
        <property type="protein sequence ID" value="CAD8205013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XVC4"/>
    <dbReference type="OrthoDB" id="426293at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="174"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="270"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="319"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="351"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="6">
        <location>
            <begin position="457"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8205013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="793" mass="94682" checksum="E15F55CD5A87D124" modified="2022-10-12" version="1">MKKTKYLLQFSIKEAKHQEDKKIELEAMNNVEKEIHKFENSDVWKMKPLKIIQKIAIFITKMKHFSPIYRFKILNKNIFYLIRDRTSSFQYYLYSRLLENQPTRFGQMKYEGKLTNAIWWRIWSFLKSNQTVLLPADKFLFVWDVILMFVTIVNILYVPLQLSFDLNKEEIGNAYLLFSTIPSCIFLIELILNFFKGYYVRGILHTSKRDIFWHYVKGEFLIDLTVVLPFILSWFGYSFSNYLMLIRMTKVRRTMIVIEEISNFKEKTAVIYSLFCLIYSLLLISHFCACLFHYFAILEVDNGYSHTWLHQQGIYEADAYVKYFTSLYWVTITSMTVGYGDIVPVTTPEKILVTFLTFLVVGTFGYALGMIQSIFYKLAEQQNLNNAKLRLVSNHIKQRGLNTQLQFRVRKYIEYYLQFKQEEELDLDELMGQLNPKLKQEVQIAMYYSYFKNSKLLGSNLSEEILKKLCFCVHEKTYAPEEFIIKKDDHPDKLYIVLSGRIKSVLLDRTIKRYISGKLVCEREFFFQDQMQFDIVAQTFVQVAYINQTDFLNILQKDTRQYEKYRLILDGTQYGDNNNQIICEACSSHHQFKHCPLVFFRKNMNKVISIYNSSIDHERQMYIRQRKKRRFNSLMIKERALEQILEQNKSIGVVDNKFLIRLGLQRNDEDEESYPDQTVVQHSQQPLQNQSEQRFVFKNRIARQVSHRCLLVPTTSCNNLQENQEDNQTQMVNGEANIDKVEEYDYYYPHYNITKVIKLINNYNIYSRVLDKIRGHKNRFAQYIARQIMYKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y131</accession>
    <name>A0A8S1Y131_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1480028</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000148">
        <property type="protein sequence ID" value="CAD8207619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="301333at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="153"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1845"/>
            <end position="1872"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8207619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1899" mass="227337" checksum="AE3C51477F290043" modified="2022-10-12" version="1">MLPDSNNQLSIKLSPKLKGIIVSQLIKTQSSLTLQYAFIRWQVRTNPEIVKKAVDKILLNSKLNQFNAFQKLKFLLGTKPPKEARMNARKKKNLSIINLALFIQKKELQLKRQAIESIKPKSQDTNKMLGLLLWSISSKYRERYLREKFQWWKLIAKIKSNKLEKQLKALEKLGEEYNMRNDRLDRQRLKEAFEIWRGDLLNFRLKKKFFAVLLKTTFGRLQRCYTRWVDLPDKRENDIKKQGLLLINKLGNKVDQNKRLVWNAFKDIHDEAKNKKTKVIRELIEVTFNQSYTAFYKWANYNTYAKLTETNLKKIKSLQQSANVTHQLVKQETYKLFNLSNKMQICSFLDKFILKLQQRQKLDALRQIQNYCVQKKMEEKLNNINKQDLISKLKDTANKTEKGLLKQILKKFQINKINEEIKNKYFSRILLSVNGQLIDSFRKWKTLPNPHDINNIQKASKFQIKLQMFIKNKNKQAYDPLKYLHYDALQKKRFCIRQLFSVTMSAPQRYLKQWQNVTKVYKSVIACQKTNNFFYSLAMIIQSNMLIFIQNKKDIDNKEKCIQKILASQHSNLAIAFFRWKSYNKQEQILERLGDEKMKFFILNLKKFLENDKKQRLRRTLNQFHQNLQIAQQIKKINLRLMQTVIGQVSSSFQKWKYLPEDKINGQTAKVSKFMISLGRVAYRFVKVNSWNIMEDYLLDGQAKKKYCINKIISIGQSDIKTAFLIWHRRSWEMKMFDQFSQLNVTLNDEIIKQRLVNWINSGYKVKHYALIEILKRFHQNAESQNLRRKALAIIHRNTISQVWISFNKWKQIPEFDYTQLAKVTKFEQSFNHFLIRNIKKKTWNPLHEIYDDALAIKKRAVLLLVKTQESEQQKALSKWNKNVCLIQEVQRCKSVIQLFKFIGNQIQCNLQIMQPNTESRLKEKAILKIIEGNNDNIRYFFMRWINYIKFERIQAIEGEKKEFLIRQIGFFYKNNIKSKLRLVLSKFKRNSILSAVQQKFFSRLFATKFGGAIVAFQKWKNLPEIQNYEDIKKGRKLEKLLENLFRGRIKLSYDPLKDEYQEAMNKKMHCIRKLIILGMGLNKRLFLQWRNTNRLLKSIETCRQTTNFFQTLAFTVSGNVQVIFKTNQLQEKVFQKMLTQYTNLLRWAFIKWNNQAKSDKIASLMDEEKKKFLLFALQRNLKHNEIGQLRDILRKFNDGRNRLNLIKKLQLKLLHTFAGQMETSFLKWKQLPDQSNIKQASIFEQKINSFKMQILKKNSFKYLQNIYYDGQAKQKYAINKMMFNCMSVEKKVFQQWNKVSELQKTEERASKVFRFFGSINQIQQNHMYFFYEDQIKQLKKQEELVRLLKEWEDMFKDSYQKWKQTQAKLDLLASQNRQKQKFSIIEILKDYIQNKKNYNLRLILRKFSNSNRSQKAVNKFLLGISNSSIGQLHNSFQMWKRLPEPQKTKQGYIFEKKMFSLFRRQLLLSFDSLQDIYFQALNKKKRVLLLLVRCTKSQQQQAVQRWRDAIKQINQQEIQNVQENRKLAIQLFYQHKMNQLNVAFIEWRDTIRINVNKQENKTQNQERLKKEAILLFQNYAYVRLRVYFTQWKIRAVKRNMIQICQAIQRMIQLNRFSEKAIKKYVMDIWRGQPSKNKWFKRVADIIAKNSRISKQVAYWRMRDNSLNQKAVGLSTQQIIKFKKLFNNLCKAFDRIKQRAFTHLEHYGKGIPDDNSFQPSHSSFLQQTPIKEAFFKSDIDSIISKNGQKMALNTISRIFRIYFKQRFIEFMIELDDQKQKSISTPQKKGAEKLFAILEQNLKLKQQVICFQISQVNEQGVLNQINEIINYQNSTQSASRLELSQIKEQQIKLNEQERMIEDLKEKIRCMAINRLVLALEKYEDHFVEDAFLGISEYQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y1V1</accession>
    <name>A0A8S1Y1V1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cilia- and flagella-associated protein 299</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1410018</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in spermatogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000141">
        <property type="protein sequence ID" value="CAD8205554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y1V1"/>
    <dbReference type="OrthoDB" id="2136125at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027887">
        <property type="entry name" value="DUF4464"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33588">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 299"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33588:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 299"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14713">
        <property type="entry name" value="DUF4464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8205554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="240" mass="28113" checksum="F47F950A6753139E" modified="2022-10-12" version="1">MMIAEDFDSEVYDTSLDKFKTYEEYLDSHITKEDLFYLEDIELARQLKEQGYHAKTEILSREQFEAKKKAVEEARLNQNKDKTKALAHTEVSDPSIIEKSPFLRALAERELKVLNGKLLTIIFIRLESANCEVSGYIDYAHRLKTEDFKIYFEGKKKLQPRPTDLSYYNWRTGQCVSNDSPNFKVDANSGQQGLLFRNKRDRKVINVDPNKKPEDGMTRTEIESPEAIQIVLYDYYTRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y2D0</accession>
    <name>A0A8S1Y2D0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">TOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T1480138</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000148">
        <property type="protein sequence ID" value="CAD8207823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y2D0"/>
    <dbReference type="OrthoDB" id="543373at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001121">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058584">
        <property type="entry name" value="IMB1_TNPO1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041653">
        <property type="entry name" value="Importin_rep_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041389">
        <property type="entry name" value="Importin_rep_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057672">
        <property type="entry name" value="TPR_IPO4/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18808">
        <property type="entry name" value="Importin_rep_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18829">
        <property type="entry name" value="Importin_rep_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25574">
        <property type="entry name" value="TPR_IMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25780">
        <property type="entry name" value="TPR_IPO5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="TOG" evidence="10">
        <location>
            <begin position="350"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="8">
        <location>
            <begin position="406"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="774"/>
            <end position="801"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8207823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1077" mass="122360" checksum="7EC323A4FBCF5842" modified="2022-10-12" version="1">MSLQEQLLNVIHGVFSSDNATRQKAEELLAQYRDSQPSEFVTAMLHLCRHEELKIRQFAPVYLRNSLSNYSPKSHKNVWSLLAPETQEIVKVSLFQLLELETSSNVRSQLCDTIGELGGSLFEDETKNSWPNLLQTLWQLFLSPKNDLIECGFKILANLFTYAIDLFNKHQADLHTLFIQGLASPDQKIKTATIQAIGNYVTTSEPKQYRVFQDLIPNLMQSALSVTIQDQTLGEDIMETFSEIVDAEPKFFRKQINVFFNGIAAIFRESQIEQGLKRIGTETLISLAEKFPRVFKQDKQYLSQLVEMIFFHMIQISQTVTEEWMKPAEGFNDDIQQDEDCETTRFGMSSIDRLIESIGDKEMLPVLSPIVNQLLQHQDWRYKYAAILALSQVGEYIEEVAEVKPIIELVSPMLSDSNPMIRYAVCHAIGQIADDMKPKFQESYLHLIVPQFLTRLQVEDVPRVTSHILAALTNFVEGTEKGIESYLQNLIQLTIQYLNNGISIVKENAMSALAATAESSKQQFLPYVNEIVPLLFQVFQNHQNKEYRQLKGQTIETITLIASAVGQASFQPFLAETVRILIQVQTSQLEAVDPQKSYVLSGWQRLALVCPQQIAVYLPEIIPSLFQLVQQVFKVHTGTADEEFHTYDNEEAEVAIHMLSVFIEELKESFFPYFDSCTQLIVPLCNFNTDENIRSAACKCLVSLIENVKATNNVQQLVNGAKYFLGIILEAAEKEFDPMVIIEQVDCIKEIIDIVGQPFMTTEEVTQLSDKVFKLLLESDKRKAENEKMSKEEDVDEDEKTVIKEETETEEELHVKIAECIGSIFKTHKDQVQPLYEVICNQILPKVLDPAQSPKMHQFGIFLIDDMVEYLGYPYVQAKLNDFAQALTIYAVDKVCFVRQAAVYGIGIMALNTPEQLYINVAPMLSKALVDSLKVDKNQDDTEKQHGHARDNSIAALGKIIKYQSKSLGGDLAQGIQTWLHLLPLKYDKQEARIQHEQLAEFVIADCNQLINGKPENALQILKVFANSYKTKRSSEAIDTKISSALKIFEQSQGQNVQAIFGMLSQEEQKKLLEVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y4T9</accession>
    <name>A0A8S1Y4T9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1450044</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000145">
        <property type="protein sequence ID" value="CAD8206764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y4T9"/>
    <dbReference type="OrthoDB" id="294796at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8206764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="459" mass="54270" checksum="AF9577F57A9C6A8C" modified="2022-10-12" version="1">MHDQKCLFQKLEGTLAEKLCIKELLKEQVIIYVQKTKEFIQKEMIISFNALIIDNQYIELSKCQFEERVSKPKNNEGINVIIIKSTAGKEYLITSNSQQCLRIKIYLKKFCVNKNYSGKYRLQEQKNCLPLSQSYKKNNQYFSTIKFEKKQLEENEELQMFHNQLTIMHAFQQIPNVIRVCEDAQRYYILGENMKLQSLESLLLNGFEFKEVPIVSIIFMILQTIQKFQAKTIYHGNINLQNIYVNINSPTLQIASLFPKYKEHNIKNIEKDIYNVGKLLYQITFYTQRGEIPQDINLNLIQSLMDQWSQPNSQRTQYRFLYRVSQLNLLNQLLSPGITVEVALIHQWFVNIKQNLKIEPKQLMSKAFLTTIMEEQEFHMTSSQILDHRVTITQQSYQEYEPDEELYPIRCQLINTTQMIPSKKKHDDAKISLFRSKTFTSSTIKRDSLHEITKQSTFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YDD4</accession>
    <name>A0A8S1YDD4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1650046</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000165">
        <property type="protein sequence ID" value="CAD8211800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YDD4"/>
    <dbReference type="OrthoDB" id="283334at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8211800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="149" mass="17432" checksum="2AE532D054D4FFBD" modified="2022-10-12" version="1">MKQPILNKGSKPEKNPTSYNNDGFGRDTYISYNNGGNFGTEFRFLTVHQNRTGMNMMPSPASRAYEVPQRLFYQCDGTGRDTYVLLNVQEKYSPMQNYKRMLRSSDQFFSSPKYRYELPPLAREKFKRTQQSQRSLISRLSQPKLKQSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YFU7</accession>
    <name>A0A8S1YFU7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1590017</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000159">
        <property type="protein sequence ID" value="CAD8210354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YFU7"/>
    <dbReference type="OrthoDB" id="290481at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8210354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="84" mass="10078" checksum="1B2B48DCE3C8A128" modified="2022-10-12" version="1">MILPPLIIEARSVSLKKQEINLKQENIFTNVFSSNRCQHILNLQRKKKMLQNQLQIVNKSHFTNDQLSEIYYFRSKLNKQFKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YPU1</accession>
    <name>A0A8S1YPU1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T2730001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000273">
        <property type="protein sequence ID" value="CAD8214917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YPU1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8214917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="71" mass="7811" checksum="86C52EF624C7ED8A" modified="2022-10-12" version="1">MLSTSGCSGSLNKITTLSPHNGQITCLQISRKNNSFLSTGFINQYVVGINQIIKNERIPNYDDNILIAQIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RUP1</accession>
    <name>A0A8S1RUP1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0010276</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RUP1"/>
    <dbReference type="OrthoDB" id="10252171at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001034">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="113"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="40"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8131768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="528" mass="61435" checksum="241B215599EBDDD6" modified="2022-10-12" version="1">MLDQLSQITLSESSVFETQRLPPRLQTTEKKGSGRFTFLKSESEDKQTQAQNNRDRIKQQLRGRIQLIIKKPLNDEKDLLEELQELGRKLDVEINEFDSEYICPNLKKFDEEYEMMEILGEGCLGLVKRIVHKQSQIEYAVKIVQTQDDEIIRNMILEFKSMFKLQHENIVKVHKLYVDFNNGFQSESKAHVVMELIKGKEMFEVINELGHYSESDAKELFKQLLSAIEYMHRNGICHRDLKPNNILCVENKNQIKVTDFNVSKFSDAYKEFGDLKDREKIEMWTYTGTVAFSAPEIFSGEGYNQMVDMWSAGCILYSMLSGQLPFLSDYLNDLIEKIKEAAIEFPNDLFEQVSEEAKDLIIQLLQKDWTFRPHPDAALKHQWFQIVDDDQVRKKSLRKLAIRKNMPRLSSKYSKNSHKQRNILLGSSQTITIKNNRSVDSKCDLQQADSANSFLKIPTKKSIFFSTNLGETKGVNQNQCQNQEESPFLVDQPNSSNLSDDIDEFDQCTIDRFVNFQKQYNVDDEVQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RX47</accession>
    <name>A0A8S1RX47_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">V-type proton ATPase subunit E</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0020011</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase E subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8132668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RX47"/>
    <dbReference type="OrthoDB" id="10263003at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002842">
        <property type="entry name" value="ATPase_V1_Esu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45715">
        <property type="entry name" value="ATPASE H+-TRANSPORTING V1 SUBUNIT E1A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01991">
        <property type="entry name" value="vATP-synt_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="38"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8132668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="226" mass="26481" checksum="5C0BB440375DA4D6" modified="2022-10-12" version="1">MADFNPQERVKKMVNAIKAEATEKAEQIKDMAAQQFRIEKNKLLNQQKERIIEEYKKKIESYTIEKRIQRSSKINQSRLSKMQARFELIQRLKEEVRQKMTKLIQDQSVYKELLKNLIVQGMIKLLEPRIELTCLEQDVQLVKSILGECQEEFSQIIKRETTKDFKTTLSINQSQYLTEKSGKPILGGIVLSCANNRIVCSNTLDDRLELSLQEFLPDIRNGLFRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S0Q9</accession>
    <name>A0A8S1S0Q9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SIT4 phosphatase-associated family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0020250</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAPS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S0Q9"/>
    <dbReference type="OrthoDB" id="303192at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007587">
        <property type="entry name" value="SAPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634:SF8">
        <property type="entry name" value="FIERY MOUNTAIN, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634">
        <property type="entry name" value="SIT4 YEAST -ASSOCIATING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="544"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="544"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="568"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8133140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="606" mass="71216" checksum="A878C8F916F90D0E" modified="2022-10-12" version="1">MFFNFARFFTQQQQLDSLLANPDCDLETILSTEGILQEIKGLGATKFADHVIKHPEMYQKMIDYIIQFEDELIDKKTQIQYPFLTSEILGSQNQKLIDYLFECPEEEIQNENQQIRQGLYNQLLSFLDNDILNVTSAGYFAKAFIAIIKKRGFDVWATIVKNKTILSNLIKHIDIKHIAEIIEKLIILDTSQENYEEKFFEERKQLLERIIKLLQHKSYNTEIVDNVCDILIEITTKSMSSLYYNNGEIIPFIDIIKKPELFLKIALQTQRIGPYQLLINLVEMQSKETKKEDDDEVVHNLEKDYSIFEQILSELPQNLLNQDYHTSNFINSNRSSIQPFGLARMHLLKLIQVLVQTNDYKIIKILIHYRLIEALQAIIIKYESNNQIHACCDKILRAIIDLNQDDFTFDVFENNNLLNYIVQNHNIENSKKGFIGFLTSISNYLEDKSKQNNKILKLLRMNDLWQQFTDNILNNVNSKEKPFLCNANPRPSQEPDVLDEQETDNILNILRKFNNNFQKNDKHVGGNEIGEGVQRNTEVLNEFEQDQQDQQDDNTEEEPLQYELNQQNEDDQPEEDDQVQQEYNQSNFWKLDVNEKLAQELLDNFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S2G1</accession>
    <name>A0A8S1S2G1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0020222</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S2G1"/>
    <dbReference type="OrthoDB" id="432109at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005879">
        <property type="term" value="C:axonemal microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030992">
        <property type="term" value="C:intraciliary transport particle B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120170">
        <property type="term" value="F:intraciliary transport particle B binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000418">
        <property type="entry name" value="Tetratricopeptide repeat protein 30A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039941">
        <property type="entry name" value="TT30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20931">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20931:SF0">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13174">
        <property type="entry name" value="TPR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8133084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="626" mass="73258" checksum="901B105B79AD5BCC" modified="2022-10-12" version="1">MQPIHPIIEGQRTRTIYNLIKDQKYKDAINYLNYELQFCPKSRPLSLLAYCHYMNQDFSQVVTIYEQLVKYYPEIDDYKIYIAQSYYKDSLYDEALKICASVENSQYQGKILQLQALIRYEKKMNFNMQKHYLNKMIWMILIQQQMKLNKFEEARQRFQDAMNLTGYSCELSYNIALCYYKQKQLAQSLKFIAEIIERGVREHPQLGVGSNSEGIEVKSVGNSQALKESALIEAFNLKAAIEYSIKNYSAAKEALIDMPPREEEELDPQTAEGFKKLNHLLQNPPFPSETFSNLLLLYCKYGYFDMAADILAENADLTFNTISQDDFEFVDALILTASSPEESFRKFQLLANKHIDTLRRITKSIQDSRLNRDNKGIKKSLKEFDDCLEKFIPVLMAQAKIYWDKDNYQQVEKLFRQSAEFCADHEVWKLNLAHVFFVQDNKYREAIRYQEPIVKKNNDNLLSLTAIVVANLCVSYIMVNQNEDAEELMKKLEAEEQKSQVQEPDKPVYHLCIVNLVIGILYCSKNNYEFGVSRVIKFLEPYNKKINTDFWFYAKRCFLALIEVLAKHMIILKDTSYSEILDFLDAADSFGKAIPSVINPLEQMNEKHTVSYEARMIKRIILKLRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SEH8</accession>
    <name>A0A8S1SEH8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0080125</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SEH8"/>
    <dbReference type="OrthoDB" id="311128at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="111"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="33"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="127"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="137"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="155"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8139491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="178" mass="21030" checksum="F10AB25A2D58110E" modified="2022-10-12" version="1">MKYIPLTQHFQTVAMLYEKCADLSSQIDSLQSQISNNEGQNRQMENEEIDRLPQINQDPLSKAPEKTNTQSQIEKRVQQIDEIEEEKQNQEKTVLKIGKSIEKSNKMKVSLTCDHCDKIFETEKQYKKHRYRINYKGPKDKSKSKSQKDHKNKKENKEKKDKRESSSKKSFETFQICL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SFX8</accession>
    <name>A0A8S1SFX8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Copine</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0080017</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SFX8"/>
    <dbReference type="OrthoDB" id="298824at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04047">
        <property type="entry name" value="C2B_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037768">
        <property type="entry name" value="C2B_Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045052">
        <property type="entry name" value="Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010734">
        <property type="entry name" value="Copine_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857:SF106">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857">
        <property type="entry name" value="COPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07002">
        <property type="entry name" value="Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="4">
        <location>
            <begin position="277"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="692"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="693"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="705"/>
            <end position="714"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8139275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="714" mass="83353" checksum="DF8E9382465D2A96" modified="2022-10-12" version="1">MSSQQKFFVVRIKCYELIPIQNVSEINSVVIVKEQADNKWNEKGKTEIQLNQRNPQFSTSIQLNYQFEISYKVLFEVYNCQQEKRHLIGYTEVNIQTLLAAKDQIYYTDICNKNKKVGRIVIIGEYQKYKNDMVELILSAESLSRKCCIFNLSPSQPYIKISRTFQDTNESNLVYQSEPIKYPERRTWPEIKKMARDICNNDSDAVLQIDIYDKGRSNDIYIGSVNLSISQMLDKKGYRNLQHKGNQSIIGQLSIQKCLFREIPTFEQYLQEGLQLNLMISIDFTDSNLEPTNPQSLHYFDVTNKRLSQYEQALRNVSEILIQYDHDKRVPLFGFGFEMNGKAHHCYPLNHNLEDPEVVNILGLFQTYRNFVGTVKFSGPTLFRPTLKEAMKTAKGFKDSGSEKYVVLAILTDGIISDFDGSFEMIVDCCELPISIIIIGIGDADFTLMERLNNNELNEIDSQGRKATRDLVKFVVFNNYKNEQKKFAQEVLSELPDQVVNYMSTIGKMPGTREQKQQPFIVQNYQFDQYYQPKGIQRQNTLKRLLGEPDEEYQPDERTIVTQNQQQFMQPQPYLYQQQQDQGKGQQVFGQHLYHQIYNNVQNPLQTEQENFKQPYTSQNLYNLNQDQQTKNINQQSTFLNGLMSNHNNTQIIQGTNIQPIQSISHIIEHPDINKQRQHSDVSFPLIDNDDQQQEHIQRDEERAQYNSNQKENH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJT7</accession>
    <name>A0A8S1SJT7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0080442</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8140080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJT7"/>
    <dbReference type="OrthoDB" id="293641at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8140080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="304" mass="36089" checksum="1E3649E625C5CDDE" modified="2022-10-12" version="1">MLNLKESQDCKKGKKLDSATLMQIICFFLLLVNHNLNGMTQYSEVSIFQADQFRIPEEHSIEDEYINFGDTNNFAFSDIDFEMNKWLPIEFSFIDNMEMEDQFTTGQQQTQQFKPISMQQQYDILFEFKSSIKSKVIAGSDLFSKILDYANKYQLNKFKSIPKINDKNQKKINHSIRIKGNKQHKFMISKLESWDVHLKSIRTHFLQKIFNKLWKVPTQQILKAHCFSGKNSQQNKIHIIVYLQNEIEQIEKQYSDFQLKQEIYQNIIQRIIFCLDLATQLNNQADDLIFDILFKGFIAELFHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SN47</accession>
    <name>A0A8S1SN47_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0090320</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SN47"/>
    <dbReference type="OrthoDB" id="415459at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051252">
        <property type="term" value="P:regulation of RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000003">
        <property type="entry name" value="Zinc finger CCCH domain-containing protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF18">
        <property type="entry name" value="PROTEIN TIS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25427">
        <property type="entry name" value="zf-CCCH_UNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="5">
        <location>
            <begin position="13"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="5">
        <location>
            <begin position="79"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="13"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="79"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8140866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="222" mass="25782" checksum="577951A0BD6B7C2C" modified="2022-10-12" version="1">MYQNLQAQQPNSKYKTQLCRHFITIGSCALGTRCQFAHGRQELRTNNQVMTNYIQPNMQQNNQVGTQNRVQNINPMIANYKTQLCKHFNPQTGQCKNGSTCTFAHGEQELNQINPYLQNQYMLMQKQIKQQNQATLQAELTQQILIMILTNMEAIFPGQNNILNLLRLGQEKAKQGDNQTASEIIKQIIHDESRTKEEKSQYQLIYNNAQNHYNQKLKECNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SP15</accession>
    <name>A0A8S1SP15_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0110052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SP15"/>
    <dbReference type="OrthoDB" id="674604at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001434">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="233"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="278"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8142263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="497" mass="58026" checksum="2E345DCECFB8D411" modified="2022-10-12" version="1">MQIRCISADHQNQQITGFCIDSKCPNQRHYCKVCLPSHNLHLNKLISQELLYQWVKERILMLKYLKNNVQEFKFALDSLINLFLPFNNFNIQQLSELELSQIDQLITGLCQMADCEEELFKQLQQSIEQTKSIVNQILKKLKSQTNQNQNGNLQIPQFNIDKSTLEQTNNQSILEPNVKLFTFDFMKQNIIKQNESCNAIAFNKDYSIVITGCKKDIKVFQNNQGKLNQIQLLSEHTSNVYSLNFLKNINNFVSGSDDSSIIIWQAIGNNQWQSQQKLNGHSLAIFCLLLNNTDDLIISGSADKTIKIWVKQDQWLCQQTITDHTNQVYSISLNEEQNKLISCSFDYQILVIEQQNMDKKWIVKQQIKLDTWGYRLCQINDNQFTFQPYCKQQMHVYEMDNNTKQYRKTKEIAVKSGSSVDNCFFPQQYLKSKCLLVNKNGNYINLMRKTENGDFKVQQSIKIGNFHIYGQLSNDGEYLITWDDGSKEIQIRKCREL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SQX3</accession>
    <name>A0A8S1SQX3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0120466</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SQX3"/>
    <dbReference type="OrthoDB" id="10336362at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="125"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="185"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="357"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8143911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="596" mass="69968" checksum="44B2B9A2B9B18DCF" modified="2022-10-12" version="1">MKLSRIPVRAFGNAVYQQPIEQRQVRPSSIQQSLEPRQIMRCVSQEQKLPHPPIQYVQYPQQRGSSPPIRKSLQETVAYYHQQQYQQNVPLKTEQQNKLSELNKVQSQAQLNFSIQDDTGQSTIIKQQNEQIQQLQTQLSEKVHQYQQLQERFNIEFKKFQEDLRHKLNNIDLNITQNDYTQQFMLEIDRKVSNLQNNLNEQIRELQENFEQKTKQSEHLVKNLSSNTQLRLNEDEQHFKQEIENLNIKLNSKADRQKVEQTIQVQITQLQNQYQLQLQQAHQLLNKINNEKSDELLQIKNQIQNTLLNLTQQVNQQLQNMYDTINQRDQENNNNKQESKYLSQHFNNLSNKFQQLQQQSIETITQLKQKNKQIEKEKQELNKQLTQQQFLVSQFQSEINKLKNTNSQLTLQLSPQVKQNSFLSNSTAVANQKSKKQLSIAKKKNEISLDDFADFNENSLLDSNDSFEINETCGFSKTKKTIQIEEFQSPPRNPFKKSQLQNINKLLNENLSGQMSCKSRGKSAPLPMINALTKAIKRRLLYLGKNDRILTCTYDSEKHLIDCDMGLCLLDENGSPFVVSDQEKNQLIQMKLIRIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SS52</accession>
    <name>A0A8S1SS52_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">alpha-mannosidase</fullName>
            <ecNumber evidence="4">3.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0110312</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.</text>
            <dbReference type="EC" id="3.2.1.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 38 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.24" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SS52"/>
    <dbReference type="OrthoDB" id="441398at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10810">
        <property type="entry name" value="GH38N_AMII_LAM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.50:FF:000002">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.50:FF:000003">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.110.10:FF:000001">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.30:FF:000011">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011682">
        <property type="entry name" value="Glyco_hydro_38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015341">
        <property type="entry name" value="Glyco_hydro_38_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000602">
        <property type="entry name" value="Glyco_hydro_38_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050843">
        <property type="entry name" value="Glycosyl_Hydrlase_38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607">
        <property type="entry name" value="ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607:SF3">
        <property type="entry name" value="LYSOSOMAL ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09261">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07748">
        <property type="entry name" value="Glyco_hydro_38C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01074">
        <property type="entry name" value="Glyco_hydro_38N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00872">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Glycoside hydrolase family 38 central" evidence="11">
        <location>
            <begin position="357"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8142738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="958" mass="113697" checksum="4AC572679FD0E6E0" modified="2022-10-12" version="1">MKQILFISFYVFNVLCNLRFRDKEQLERHHSHTEVVDEFLTVHVIPHSHDDVGWLKTVEQYFYGLRNDYQWANVGYVIDSYIEELQRDENRKFIQVEIKFFKMWWDLQNETTRNITKQLVENRQLQFISGGWCMNDEATTYYEDIIDQMSLGHKFLKDTFNYIPNIGWQIDPFGHQNSQAALSYQMGFDAWFFARIDFEDKETRLDNKNMEIIMLPPAEGVQYPIFTHIQYYHYEAPPTFNFDILRRSDAIVDNQKSESYNIKERSDQLVSYFKSQSLHYKNDHLIHTFGSDFCWSNSKVFYQNLDKLIKYINMNPEYNMKLQYSTPEEYIAEINKQNTIYETKSDDFFPYSDEPHAFWTGYFTSRVAIKGYVKRLGRQAQTYRKFIALLYQQELIQDNYQNITDTIYELDNALAICQHHDAVTGTEKQHVNDDYINILYKGEQHIQKLTRKFLSQLLYNTTTIDYEQCEYNITAQQCSKTYNALKANKTVILTIIDGKVKQENNTDIIRIKVPNLKLLIKDQNNQVLYGDIDCTNGFQDCDLYFKYQIPLNNVIQYFTIQPVLYNESASEIQLTVNPFPYNEDTLITLNSTKSFKLKYAYYQSYQQKDQADGAYIFRPSNNSKIMYGNISYTAIGYGRIMSIFRIVRTYTKSVVKNFYHQKDTFDIQTFIDQIPISDQIGKNIIMVIEVPNFNSDNTFYTDSNGLQFQKRIINYRPQYNYSLHENVSGNYYPITSGIYIQDGQECVGLLNDRSQGGSSLQNGQIEIMIQRRLLQDDSRGVGEALKENDEKGYGLVQNIQHKLTFFNIEQNPNQIRKLQYYLDHQPLVFFNFDQQLPLIPQKLNWFLPFDGFNIDPLLIYGDNLLKFNYILWNEDEILLRIQNMQEKFSMKIDKMGFEGQAIRTTLTGNQKWSDWKNKSLKWNYNKQENTLDQDLDDVLYPLQIQTYLIRNSELFYQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SVH5</accession>
    <name>A0A8S1SVH5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0130039</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SVH5"/>
    <dbReference type="OrthoDB" id="284165at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033897">
        <property type="term" value="F:ribonuclease T2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00374">
        <property type="entry name" value="RNase_T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.730.10:FF:000017">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001568">
        <property type="entry name" value="RNase_T2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033130">
        <property type="entry name" value="RNase_T2_His_AS_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11240">
        <property type="entry name" value="RIBONUCLEASE T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11240:SF22">
        <property type="entry name" value="RIBONUCLEASE T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00445">
        <property type="entry name" value="Ribonuclease_T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00531">
        <property type="entry name" value="RNASE_T2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035727053" evidence="1">
        <location>
            <begin position="18"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8144110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="254" mass="29268" checksum="C38643A16E935E33" modified="2022-10-12" version="1">MKTTILLLLYIINQAYCQQNDEILGYMYVFAQEWPGSICKFQKCTKTYMGNYDNARWNTHGLWPNTMLQTSCGMIFNCKDEEYDEDKLTIATKTLIDVTWNGMYSDTFTFRKHEWEKHGTCHPDNLSQNGYMSRVGNLNNQYNYYKILASAGIYPDDDRQLTDGEVRAAFTKVLGISTAMSYTCQKDSSTGKFYIAELRTCFTQAMKPRTCDCSKPVSAFVTCGKTFYYPTFLLSVDEQIELEEIPDLSNGFLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T2B1</accession>
    <name>A0A8S1T2B1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Group 1 truncated hemoglobin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0140346</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T2B1"/>
    <dbReference type="OrthoDB" id="296865at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005344">
        <property type="term" value="F:oxygen carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00454">
        <property type="entry name" value="TrHb1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044203">
        <property type="entry name" value="GlbO/GLB3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001486">
        <property type="entry name" value="Hemoglobin_trunc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47366:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_37, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47366">
        <property type="entry name" value="TWO-ON-TWO HEMOGLOBIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01152">
        <property type="entry name" value="Bac_globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8145639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="124" mass="14977" checksum="5643960D843E64FD" modified="2022-10-12" version="1">MDNQKKCLFEKFGGDQQVSELIDQFYYKVLFDKLLRDKFLKADMSRVRYQQKRFFAQMMGDSNTQYTGRDLIEVHKNLNITNQQFDKFKTHLKNIALDMEIPNLDIEELLQHVEKHRNLIVFQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T2Q0</accession>
    <name>A0A8S1T2Q0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0150413</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T2Q0"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="305"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="637"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8146573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="771" mass="88870" checksum="B7C88F34A1F33DA2" modified="2022-10-12" version="1">MNSCIKSIGSAKVLSIMLIADSLLIHIQSYLISNYNYLIIALSLTCFHGIVLTSEHFLQGRNKLLNVFFNASKGVAQIIVHYLLEQNWLIFAGIQIICIMKQDVLHIKYVSSIFFITQIFCVAFNKTDFIIVGVIRTVFNYILISQIFRFESKNTLIQLENILKHIQYDEFCLLDSYFNPIFNQQIFDKLKEEQVVGVQDFYIQTRIITPRDNTTNLIREIPLNCEEEAKQTLSIQDIIFHLKKNKVPLTQKLCYQLNNQQNYHIQIENIILDENNSMIMIQKRKKCENIQQGNQINLMIKTVRKVSHDMRNPLNAIINMQMCLKEQIDKELVMKYLKPSLNSCHLLLNLLNDILDSAQVENKKIKLVYRRFNLQKLIQKTISIFDSLKDKKDIIITFNYDSKIPIEVNSDKFRIRQIIMNLLSNAIKYTKPNGKVYIDCSESSQRKNFIKICIQDTGFGIKQENLKYLFKEYSKIEEGENQNLNPFGIGLGLMISNELAKLLSNNGIQVQSEVGIGSIFYFEIENKQLAPEDISESQLLIIHQNGLPSFDVHFIPGPNSNKSPVISLALPQVESSKKIILKRSQKTINIMTNSINNQTRLNVKSDEKSIARKQSLQAKQIQALRDKWYDYTNQKPPILIVDDDEVNIMVLQYLMEQMNISSDSAMNGMACLQKFVERQKEGLSYQLVFLDINMPEMNGFELAKKLIQIDPLINLIACSGDAPNSSYVEECKQSGIIGSILKPILKSKLKELLLKLSEGIKYDSQQFSYYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T945</accession>
    <name>A0A8S1T945_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0190293</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T945"/>
    <dbReference type="OrthoDB" id="608866at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41733:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_30, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41733">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED/TRANSLATION ELONGATION FACTOR EF1B, N-TERMINAL, EUKARYOTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="27"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8149785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="182" mass="21447" checksum="9505A2F001B8A195" modified="2022-10-12" version="1">MEKQKRKKHGAHNESKSLWNYTLSPGWSQEEVKILKLALQKFGIGKWRSIIQSECLPDKSIGQIYIQTQRMLGQQSLGDFMGLQIDLEKVWIDNNQKKGVMRKNGCVINTGDNPNKEERKQRIEENRQKLIKQSCLALRMIVQLNISQFKKLKMINLQLQKNYNILQILKQRLIKNLKRFNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T9I5</accession>
    <name>A0A8S1T9I5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0170232</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T9I5"/>
    <dbReference type="OrthoDB" id="303439at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8147966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="120" mass="14270" checksum="4CDE2DB71C1C1696" modified="2022-10-12" version="1">MHFQLSQNMSLDLRSPVEDGVDDDISSTEQLCDDSVESLEEFLPKIHQKSNPSFIMEEKIIQYLKQLNLLFKTDPRKKEFEYQYFLFVTLVKNKKKQYLSFQVLLRAHLLQKVSNQKLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TAM1</accession>
    <name>A0A8S1TAM1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPENT_87.1.T0170081</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TAM1"/>
    <dbReference type="OrthoDB" id="354826at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000210">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000524">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="53"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="336"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="337"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="370"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8147672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="380" mass="43563" checksum="0D41A6EC25A0EE0A" modified="2022-10-12" version="1">MGNLCETNETKKLTSVQMPSKQQDETMGYTSERNLVSSNQLESTQKKLSIEDFVMLNQLGKGAFGKVFKVKKKDNCKIYALKAMNKKQIFDSNLEQNAVIEKEVLNNNKHPFIVNLKYSFQSQTKLYFVLEYIDGGEFYQILQRTQGLPEPVVQFVAAEVILALEYLNMKLNIIYRDLKPENLLLTKSGHVKLTDFGLATKRKENNQKSYTLVGTTEYLAPEIIRKEGHSFEVDLWTLGILIYEMITGKTPFAHPERNQNKIQYLILKNNPIYPPTMSRDAKDLINSLLQTNPQLRLGANGYDSIKNHSYFSKINWQDLYEQKVKSPLKTFAEENSNRLQNLQPMSNQIKETPGNNPMPQINGISFSGNEMDRGSSVKNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TBV6</accession>
    <name>A0A8S1TBV6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Insulin-like growth factor binding protein, N-terminal</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0200015</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8149950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TBV6"/>
    <dbReference type="OrthoDB" id="409374at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035929185" description="Insulin-like growth factor binding protein, N-terminal" evidence="4">
        <location>
            <begin position="32"/>
            <end position="841"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8149950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="841" mass="97948" checksum="50A025D7E2225E29" modified="2022-10-12" version="1">MMFAPQHSNKQQAVIPLILIILFLILSDVQSRILAHSDSFTTINGWQVISNNAHESRIITCGTSNVLFLNNQSPVDYSTMYFKTFEIQPHYKIELQFNFWTIDQWNNFYFIVYYDNLLAHANFYQTISTTNFCEVSSLNDESFNTNFQHNHYGSTVSIGFLNNLGKFGISDFQLYIYECPVGCYSCDENGCFYQFLYIKSFDSRVISSINSIEGWYTNDIISTLVDNIADIYYHISTGNSFTKEITLIEHEAFSLYLRILVFNSQTTRIYIKIDDILVFTTIYNQGWLYIKDYFLDHMSSQQIKIQERRHLKSKIKITIEVVMIKINTSLQTWFGIRDFKLFLIPYNLVVDMKDTCDDSNIYPFDGCFSKMYECVEGCSNCIFGVCYECQGGWEYLETLRKCHPICGDSIITYTEECDDGNLISNDGCHFCKLTCSSQCQICQLGKCLQCQQTYQLIDNSCQLICYYNQDYEMQKQQGCYYQMENIIENEFQQHALLNSNFLMFTPNCLVQNYKIFNFQYQICNVLRINNCKTQYYQECMECIDGYHLERNKRNCISLCNDGIIVETEVCDDNNNIQFDKCYKCQQSCQLECKFCINEKCLFCLDGWQLIDHICVQECGDGIIALGSLEQCDDANDINKDGCYQCMWQCSPYCKQCINQQLCSKCIDIGFITFIFYYLFQQIQQLIQLFYQISYNFELIGENECKPICGDNIVIIGLEECDDGNEIPFDGCYQCQFQCEIGCTICEYGVCLQTCKSNQIIVNGLCKNESEDSPQIDEQDQSENNNDFSNYYNEYCGDGRKSKNEECDDGNNVNNDGCSNICLNELNWNLNVFLLLNYKYCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TDH7</accession>
    <name>A0A8S1TDH7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0190192</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TDH7"/>
    <dbReference type="OrthoDB" id="305335at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035809245" evidence="2">
        <location>
            <begin position="19"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8149604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="432" mass="49219" checksum="D16A57E062211F92" modified="2022-10-12" version="1">MKVLAMMALLLVAAYCQSNEIDVVLKMLSDLKNQTSKQLQQMESDWETTRYQKQDIVNDLMRSASTQRGECNRRDQEWANKERDIRITLSYINWLEKRIKENNERLQRLDVNRCESNSNFINDIKNSKKSLNLIAFLRRAVKNAKETDLPTLLQSAQFIQLKSFLADDGEEPEITDENNEPPHVYDEPEVVDNADQVDQEDDVLQEDETITEEQTTETQDPSDEVDQHSLVDANEEPEAQVPEHPLEAEEKATNEQLAKEAAAFNKIIEKGQKAPAETGSGKDYSQFTVAQQELLNFLDILEDQVRGSFGKKQDNQVNSAMGYSDFKGLIHKENETFKGHLVAEDVNLEKLQNQLITILQATAACKDRLKKIQNSIDLANEDLASSEAHFKSVTETLQEEQNTFDDVYRIYSSQVGSQSTSYKGDVQAKITN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TFH4</accession>
    <name>A0A8S1TFH4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PSI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0200003</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8149926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TFH4"/>
    <dbReference type="OrthoDB" id="10392879at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002895">
        <property type="entry name" value="Paramecium_SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00639">
        <property type="entry name" value="PSA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PSI" evidence="2">
        <location>
            <begin position="127"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="2">
        <location>
            <begin position="357"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="2">
        <location>
            <begin position="733"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="2">
        <location>
            <begin position="971"/>
            <end position="1012"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8149926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1089" mass="124561" checksum="791948EF00F7E3B5" modified="2022-10-12" version="1">MHTVPITAQLNGRIAYIQNRSVIKLLNVSKQLNRNNVTNFHMPAYGKWIQTEQDCKYTFPSLYMNVAQICVWQNGKCKDLNESNNLDSEQCLQNTLDTYKWTGSSCVSCFEVNWQLQFSLQLPNQCNCSQLQAEYTCLQKSSCIWSNSYCQSIECSSIMRQFQCASNPYCVWLKGQCQMFKSCTELKGTNQSDCIVLSINCPASDGINCLDSKLLLPCSAYKSQSSCTNMLGRDGFCIWQYSTCQVLRNCQQINKQQDCQYFTKQCYWSTSCQPVSCSIFNTPNTCQYYYQSINSYTPVSCYWVFGQGICAQTTDFMKQLTPATCQTNTNLTATWSNTLKICLSCLIPSPPNPKQCSCSQLISQQNCLQSQQCFWNIEINQCLQLPCDKLNQQQCIQNSKCWWKFTMQACEQLTSCSDLYAGSDFECISQSLFCPLQNKDDPPYFKCESYSQINFTNCEDLNQTQCNQYVMPPYYICQWNNYLGKCQYQSYCNQLGNPKLCYSNPFCYYQDGCKSKTCEQFNTLESCKFSINPNNWNYIQSCAWLNGSCISADNLFSEQNCYINSDYTARWSTNVKDGFCIPCNISFQQSITPKNQCLCSQLLTQYECNLSSCYWQNGFCIDNNYDCNQSNQIDCIQKAYCYWIYQKELPYGGYCNNIPQLQIQQFCTRLKGSNSLECLSQTTLCPVSIDGICQDRTYLQTCQSKNSIQLCANSIGQEGYCTYQNQSCQILTNCTQLKTISECFLFDKICVWNASSGSCSQLFCSSYTTQDSCTYIFQNINKSNIQICVWNTTTNLCTTATYIIEKSFQDCYQTSGGTYHWSNTLETIGTCVSCSLNKIQPKHTCSCTELNKFECEISKPVCKLNESNQCVKLQCSEILDSVYCSQQNECMWNLNQCVVFTKCTDLKGSSTLDCIAQSIECQYIQNGICQSTQSLHSCDKYETQECTFGIQGSDGLCYWNSLINLCSVISSCDDITNQLVCSSLTPACQWSYFFQSCVPFQCQDHNYKDQCTNVLVNLNDPQIELCVWQNNKCVPYVDSFYKLDDDICYTNSDHTYRWVKEDKHGTRCKQCLKSSLINIIAIIIILNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TG02</accession>
    <name>A0A8S1TG02_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0220311</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8152165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TG02"/>
    <dbReference type="OrthoDB" id="5970at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000950">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051186">
        <property type="entry name" value="RRM_HNRPC/RALY_subfam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13968">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13968:SF26">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="7"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="146"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8152165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="208" mass="24035" checksum="52B8D1DBD1F8BCD4" modified="2022-10-12" version="1">MGDIIPNQLFVAGYSRNKVQDEKDVRDIFKKYGTVKEVAYKGSYSFVTFNNEQEAQEALKGTNGQTYNGQKLKVDVVDNRKGRKTGPNEEDKCFKCNKGGLVIVQISNHLDVVEDDPILDQSAVIEDLNQDHILLIHHPDLEEEDIKTKERDIAEALEEINESEKEVLHQRDHLLIQLHPKDRIQTQNLDKEWEKKTHFITKEIQTIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TJX3</accession>
    <name>A0A8S1TJX3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0210365</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TJX3"/>
    <dbReference type="OrthoDB" id="447842at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047631">
        <property type="term" value="F:ADP-ribose diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035529">
        <property type="term" value="F:NADH pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000191">
        <property type="entry name" value="Hydrolase, NUDIX family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003293">
        <property type="entry name" value="Nudix_hydrolase6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040618">
        <property type="entry name" value="Pre-Nudix"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13994:SF13">
        <property type="entry name" value="FI03680P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13994">
        <property type="entry name" value="NUDIX HYDROLASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18290">
        <property type="entry name" value="Nudix_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pre-nudix hydrolase" evidence="3">
        <location>
            <begin position="15"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF18290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8151462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="217" mass="25957" checksum="825D5B1C3C8A356E" modified="2022-10-12" version="1">MQRITKKYYFSLIQKVDNYDCLHINPQSLDKYLVNQSLLKQKLEQILQDSINKNLNAIWLRLDKHQLNLSQLISDLGFQMHHCTQEYLLFSLWLQKEPSRLPNYASHKIILQYYLINSKNQIFLIDDKMPIQDVDDSSLIPIMAQTYFKQNYNINIQPLYICDLNQSINSNIMITLKCQLLEQERNQDNFVNKTISGFDQRYEINSFDKYIIYKKCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TKA9</accession>
    <name>A0A8S1TKA9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0230217</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TKA9"/>
    <dbReference type="OrthoDB" id="291502at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000833">
        <property type="entry name" value="AGC family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033931">
        <property type="entry name" value="PDK1-typ_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF163">
        <property type="entry name" value="3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14593">
        <property type="entry name" value="PH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="390"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8152740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="808" mass="95364" checksum="5485BE42D611B1C7" modified="2022-10-12" version="1">MEIDQTCTQHIKQRISWVCLEKQCSQRVMCSTCAVKIHEKSHKLEELSNLLENGVLQLYQSNKKNVSPGLKYLINGESELIQEENRIGNENNYFQSVIEKNLKGVQKKMDTVKKEFQDKQKEIRFSNQEERNKVIIELEQLNQQLKVSDDIGNNIEEAFKKIQSYVNHKEYEFKNNKEGEIVTKKIQLLNLMKDNFLKMIKTYFSNAFTIEFYKLFDMQMETLNSKEVQDVNGFLDYVDSKWKQDYKNLQTSIEKFVQKLHEGLKYIIQLPLQEEKPLKQQIIIPQFASPARNPIQYQQQPQFIPLTPFNQVPSQYQFHQQQIPLTAQRVYQNQNQFHSVPVPKAEIENEYFAQSQIIYEEPQMRNNTRPDFVSQKVETPIKKKIGIEDFIKIQSMGDGAYSTVYSCQKKEDLQKGNSNQCPIYAIKIIDKNFMKLNKKQHHAYIEKEMLQYLKYEGIIKLHSTFQDKNNLYFLLELAQDCFSEFLKLYGNQKLTHQIIQFYTAEILSILEYIHSKGIVHRDIKPENLLITEEKHLKLIDFGTAVIYDQHKVPSKIVEAIDNYRKDFVDTERKRKYSFVGTNAYLTPEMISDQPVGPGTDLWALAIIMFKMYTGTIPFKTDNQEDQEVLYQKIVKDQIIFPKSIPQQAQDLMKLFLEKDLNNRLIDYSKIKSHPYFADIDFNTLWQMNPPQPLQIHRDQRKSKTFVQEPISKIIFQEMVEKDSGFLISYFTPRLLVLQIINDIPSIHYTNPKLNDKKTVIHVTQFLKYQLLKQDKFVIIDEKQKYIFKSQNAKKWIGLIGETLNHGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TL64</accession>
    <name>A0A8S1TL64_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0260134</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8154771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TL64"/>
    <dbReference type="OrthoDB" id="293101at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8154771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="258" mass="29892" checksum="866BD13B054ED3AD" modified="2022-10-12" version="1">MENNQEPEPPSHFQIWLTFIEMVAVLVCLANNMDKLHSMKNKNLKFWFLVSMNASSAFRGISLLLIMEQPQGIWLDFLYTAPILFWIQSYIIFIGHLAICISVLANKDTNIIQYSIYYSPVCLVLSYVYFLLKDGFLERVLILQFLLYAALIIGCLYFSNKLFSDLEDDDWIVPFLTQVNKLIQTISISLALRLLIFFLIFANYVKLNIDLILIEVFIGELVPYYALTFFKIPPPRHDNDSRAGTQLELQNLEGPLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TQ34</accession>
    <name>A0A8S1TQ34_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0250343</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8154380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TQ34"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8154380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="185" mass="21818" checksum="8543DD11C5D370F8" modified="2022-10-12" version="1">MQKVLHVRWENIILNLSVYYVIIRKVSIQQNQKHIIVRRQIQRLSNLIQQIILNYILDIGDQTSEVIIYQNVRKKLIIVKEVGRLEMNLVKKDLLELYAKNVIYIILEDLDNILKIRIINVKSALDLLEIFCFHSSQIQQPLSLYIQQSLVQIVSLRVLNYSNKLLNIIKQSSAVIQINLRHLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TQ35</accession>
    <name>A0A8S1TQ35_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mur ligase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0260156</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the folylpolyglutamate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8154815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TQ35"/>
    <dbReference type="OrthoDB" id="5212574at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008841">
        <property type="term" value="F:dihydrofolate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001645">
        <property type="entry name" value="Folylpolyglutamate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018109">
        <property type="entry name" value="Folylpolyglutamate_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01499">
        <property type="entry name" value="folC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136:SF0">
        <property type="entry name" value="DIHYDROFOLATE SYNTHETASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01011">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur ligase C-terminal" evidence="6">
        <location>
            <begin position="477"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="7"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8154815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="606" mass="69807" checksum="C7B15D68160B9337" modified="2022-10-12" version="1">MNYKQLYEEMVEKYQQMEEEYNQFVEESQSLEDQQQKNIESLSKQLAQAQNSLLQQKEETQKARNELQQIQNQLEKQINKKDAQIAELQKTLQTYKMQIIDLEVDQDLNDNKIRQLEESNKDLEVKLDKVLEQLALAHTDLEASKSSSQEEIERLKQTLKENEDELTAAKCLKINITTTPEIVKMPKIDSLRANAAGFNKSLTLIQALIKDLDDKMSLIRHQKKDRATLTNIIKMTQLLGNPQQNLQFIHIAGTNGKGSVTLKMCKLMQSIESCGCYTSPHISSFRERIKVDNQMISEEYVQEHLNDFFNLAERNNLELTFFEYVTCLALKYFNDMNLKYVSWETGLGGRMDSTNIISSPLISIITTIGYDHMHILGNTLELIAKEKAGIIKQNCPVVIGPKSKPYEIFIKEAQQKNADLTIVQGEFQDFNDENNAIVMAASKILMEKYQFKLNNFEEVLKIKQPCRLEQFNIQNDKGDNIKVYLDVGHNEQAIKSVLDLLKDQKIICIYGASNDKDITSCLQLLKQNCHQVYMVQAKNNRAYQIKQLLGSDEIKILYDGDISKTTLEIVNKVCQIDQVILVIGSFYIMDEVRMILKICQTQCDFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TXV2</accession>
    <name>A0A8S1TXV2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0290323</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the recoverin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8157070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TXV2"/>
    <dbReference type="OrthoDB" id="286860at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028846">
        <property type="entry name" value="Recoverin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055">
        <property type="entry name" value="CALCIUM BINDING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055:SF178">
        <property type="entry name" value="NEUROCALCIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0519">Myristate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="224"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="260"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="301"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8157070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="434" mass="50535" checksum="8E6344D03510ABC2" modified="2022-10-12" version="1">MQIYKTSLSTQGFRPQSTEYYHRIQSANTANTIHSNRTIPQQTISSNRSQTRITTAKPIRSFIISKTIKSKPIEDEDFDEIIKTSHDQDLFKFKHKCNAPQHLRMEQIYRFIYDKKTSNKQKYSMQSKINQQALPVASGSISIMLNVARKDYGFNLQSFKKKIAPKSKFKMKWKTIQWLLKNRKDAIRQIFQNYQSIVKQAKDFPDGLNREQFQGLLISFGLGADKNLAEKLFYVFDEDSSGTVDYKELIVGLEVLKDDTIDEKLKIFFDLCDEDGSGKVSEKEIFNILKQNIINENDKYQLKMVIREMIKQVDQDGDGELNKEEILQAASKNPILRRLLEQTISNVRRIDAIIQNDLEEPFHQFVPSSANFIQQKEGIHFATQQKLIDALEEIDKIHEKGKKIKEYTKPENQMGLTTYQGLQEKKFLDDSQFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TY15</accession>
    <name>A0A8S1TY15_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0280281</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8156357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TY15"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8156357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="209" mass="24861" checksum="016C41A65679B488" modified="2022-10-12" version="1">MQEKIDKEMIEALSQYFLSEYPVNGQPIFQGLYDTLQNALRQREQERDKEEKSVTKDNSKKASPTQPLCSRRFISCFAEKTFSRSLTNQKQPKQYKEKVLSFCQSQIDSEMIEETPQPNIVHGRKANSAASSLNPGTQWEKENYWVYNLKLNFILLWKITLKYSFLISLCDIVKWTKNIQQREQSFLKDRIQKWIKFLLLFKKCHQILF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TZ18</accession>
    <name>A0A8S1TZ18_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0320270</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8159011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TZ18"/>
    <dbReference type="OrthoDB" id="287754at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8159011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="349" mass="41725" checksum="BED65E7701A17BEB" modified="2022-10-12" version="1">MRETAFSSSPFEKPNKLTSGLFRDCQRFIPRRKFTEENQENATVFAKLRRAGFEKKKALKYCQAQHCKLDATQNDFGPPGYKIFKQNYDPIKQYKGWQLDGKIKLKDQCEYERISKDINLGLRQQPNEDLLAETHRNTEEKCNFTDLSRQELIMKLRSLTRDDNRKYNTPIYQLRRHNEIYYELADYFEKQQPPQIKSLVTELEAFDDIVPRKQLKQFYLEQKSTGNKKKNLNQEVSEKMQRRSRILMELTALFSPVKSDSLNRRSFFTRQDQMWQVQNDSAITSLLDMDESNVGRNAERNSIFFYRFAKVKQSEEQERINQFQDSLKKVIKKNIENINYSKKKNQFFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U1Q0</accession>
    <name>A0A8S1U1Q0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex</fullName>
            <ecNumber evidence="6">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0320057</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8158620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U1Q0"/>
    <dbReference type="OrthoDB" id="202158at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031405">
        <property type="term" value="F:lipoic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000007">
        <property type="entry name" value="Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050743">
        <property type="entry name" value="2-oxoacid_DH_E2_comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004167">
        <property type="entry name" value="PSBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43178">
        <property type="entry name" value="DIHYDROLIPOAMIDE ACETYLTRANSFERASE COMPONENT OF PYRUVATE DEHYDROGENASE COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43178:SF5">
        <property type="entry name" value="LIPOAMIDE ACYLTRANSFERASE COMPONENT OF BRANCHED-CHAIN ALPHA-KETO ACID DEHYDROGENASE COMPLEX, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02817">
        <property type="entry name" value="E3_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51826">
        <property type="entry name" value="PSBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4 6" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="8">
        <location>
            <begin position="11"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Peripheral subunit-binding (PSBD)" evidence="9">
        <location>
            <begin position="126"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="94"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8158620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="404" mass="45610" checksum="9ADB02C4C95A35F1" modified="2022-10-12" version="1">MLKIIKYFGTIKHFKLPDLGEKIKEATIVKWHVKEGDKITEFDPVADVSTDKMFTQIPSSFTGVIHKRYHKEQDQCQVGELFVDIDVDEISSVSNQKNQTTQQSSQAPTPQIASKPTPQRESLLQRISPSAKYLALQNNIDINNVKGTGIYGTITKDDISNYQEQPKTTQQINSISQSQTIKMSDFQKGMQKSMTESNTIPHLYLQEEIDVTSLSSFREELKKQQNITFMTLFIKSFSLALLQFPILNSTYDPSVPFQFITHQDHNISIAMDSPKGLVVPNIKQVQNLSILEVQQQLNKLKKLGDESKLGPNELNNGTICISNIGTIAGTYVGPLILPPQVCIVGIGRVVLQPRFIAGSYQPRKIIYTSFGCDHRILDGATIARFQNTWKQYLEQPEQMMVKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U2X3</accession>
    <name>A0A8S1U2X3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0310301</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U2X3"/>
    <dbReference type="OrthoDB" id="285328at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001034">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="79"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8158362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="440" mass="51388" checksum="2CB5E96857B99685" modified="2022-10-12" version="1">MIVENVKKNKFSFSRKSESEQQCKLEQFKNKLQGDVLQFLKNTKQDDQSLQSFLQRIGIILEGDEEKYILDIKEYHNKYIEGDILGEGCIGLVKSVKRKSDDFEFACKTVKTDSEEIVKKMIMEFKNLKRLNHPHIVSMKEIYIQWNEGFQSTGMVCVIMEKIDGREMFEVIQQQKQYSEAIKYMHENYCCHRDLKPNNILCAHDGKSIKITDFNVSKFNDSYKEFGDLNQHGKIEMWTYTGTVAFSAPEIFSGNLYNEQVDLWSAGVILYVMLSGELPFNSEYLNDLIEQIRQCKYDFAGIIWTQISESAKDLITNLLKLDPDSRFTPEQALNHPWITHEQSNSDIPRYQLEINMARILRVKNINQESKIKQICYLFGAGDIWKRHSLGQDTNLDLYDNLKKYRSIDISDSWKNAQIKVTKDQSKKGVYTIDYPFTDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U5C9</accession>
    <name>A0A8S1U5C9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0340127</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8160050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U5C9"/>
    <dbReference type="OrthoDB" id="301788at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="175"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8160050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="362" mass="43299" checksum="588DE3B51397E79B" modified="2022-10-12" version="1">MNCSIFSYYVEQTLPNYVHKKLEECFVQLSNNQNIIMCVEMICEQIEFCRKLCNRLQAQKQLLILTKLLNHQIYPIKDAEKNNTYLLSLQDVYVFQIILRMLCIMIQFESKKYHDNFDDDYIEKFNNDLVKGFLILRTNQANQLDMQFLEMFQKLKSRSVLFYKCLIKWSEQFQLRNCISSLRKLKLQLKKKQQEQIIEKRRQIDTIKQESKGECSNSFFEQNDFRIKDKRLQMIMNVKQPKQEQSFFSFQDVLKQKENTKFVNTTESTLSRPSLSKRTINGGINGKYKLGSEIKVLRKTNSSSNKKNNKSEMQPFQILQQQYQLEFCREVSRKLLKLEQKKQMNWNSEYLAADTESDSCFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U658</accession>
    <name>A0A8S1U658_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WASH1 WAHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0330216</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WASH1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U658"/>
    <dbReference type="OrthoDB" id="307871at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071203">
        <property type="term" value="C:WASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043014">
        <property type="term" value="F:alpha-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028290">
        <property type="entry name" value="WASH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021854">
        <property type="entry name" value="WASH1_WAHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23331">
        <property type="entry name" value="CXYORF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23331:SF1">
        <property type="entry name" value="WASH COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11945">
        <property type="entry name" value="WASH_WAHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WASH1 WAHD" evidence="4">
        <location>
            <begin position="12"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="208"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="280"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="447"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="296"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="312"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="345"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="447"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="466"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF11945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8159573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="477" mass="53845" checksum="0BBC788D142D74A2" modified="2022-10-12" version="1">MAFIQPCPWQFNLASFDLHHKEGLDITLGNLSLLEQATNITEEKLRDRVNLVKVRIQQLAERINVCNAKLNHLQNCPQAIVFQSPSKFVKKYEFDPTNKQSIFSECYNKPIIPIYEPYKYIRLANEQGYQGKAPDNIDRVATIQMAYGKFTQKGKTIFEGERAISKRQIQGYGTVPRKLKYVDGLSVFNQLINPYNHHTFDAREVFPEPAKQENKRKKQQADDEQVTMADAPNTIGDTITKYGEESYSYVPSNKPIAQLKVDAKLNLAGIVDYDPDNDDIDFFNPSTQQRNKKPVAPSIAPSIAPAQPPPQIQQTQQQQYQQYQQQPQQQQIQQPPPQQQQNKSAPPPPPPPPPPPPKGAPPPPPPPPPPPPPPPPPGPPPPGQLPPPPSGSRSKLLDDLNTDNPLARLKPVNKNKAVEQQKAQIKAPEMNHMDILKQQIAQRYQDLNRQPTRTTKTMRTQIIGQRVDDSDYSEHSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U8D4</accession>
    <name>A0A8S1U8D4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0360138</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U8D4"/>
    <dbReference type="OrthoDB" id="300753at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00454">
        <property type="entry name" value="TrHb1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001486">
        <property type="entry name" value="Hemoglobin_trunc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01152">
        <property type="entry name" value="Bac_globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="13"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8161365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="168" mass="18638" checksum="46D4D8A93FF669F7" modified="2022-10-12" version="1">MQKIQNFIENNKAVVAIAAVAAAIGIVGIKVLNSENRGSAKILDASIQKVPLIERLGGEQAVQPMIQILFDRISNNKELKPLFQNADKSKNSQHFNAVVLYLFGGQVKSQQNFQNHCKLNITEAQFEIILNLLDQVLQNLGVDNREINDAREAFLTMKQQIVSPNKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UAK6</accession>
    <name>A0A8S1UAK6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0360113</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UAK6"/>
    <dbReference type="OrthoDB" id="301370at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="256"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8161324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="328" mass="39947" checksum="CDB131839BF2BC1D" modified="2022-10-12" version="1">MYLPRPSSHYYPLQYPTLSELPQREQPKLIYDSRYEIEDKYYRNQIYNQQQQDLAQSQQEYQKMEKTYSKQNGYKQDLDTMNQTYGNKRNNNQQQQPPISEKYNQITDSDIKFISYIFQEEIQFRKELVQINKKLKLLKPNLLQLFFVFTSDNHYKIEKFKFLEVLQSYQDDIKVCDLELLFNYLNNWKRSQSITYGQFLQLIIPYIKYEEMPDPADLKYQQDQPTQEQMEIFCRLIQMKLQMMSTLEYYRQKIDKSLINLVQIFEFIDQDRDGYIKALEITKLLQQFGYLVTIEEAQLVLQDIDERGQININQFVYYYKPKSLAIYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UJG5</accession>
    <name>A0A8S1UJG5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0410112</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I NDUFS4 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UJG5"/>
    <dbReference type="OrthoDB" id="286255at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006885">
        <property type="entry name" value="NADH_UbQ_FeS_4_mit-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04800">
        <property type="entry name" value="NDUS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0249">Electron transport</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="1" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU367010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8164595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="186" mass="22084" checksum="482397279F61A651" modified="2022-10-12" version="1">MSIRQFVGIQEIAKKAFNSLIVIRKEIETKNQEVLAHGISVYNQVVPVDASRSKFGFFRSSRLDNRSDHRYYDMNPLPAPESKIARVYIMDDNRMHQNPTMKQGYHWNVQIERGPIHRMPWSGWTFSKDQKSRKVYKFVSLEAALEFCQQDGYGYIVQYPHFRYTSKRKYADNFKWKGFPKDEQDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UKG0</accession>
    <name>A0A8S1UKG0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0400303</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LTV1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8164262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UKG0"/>
    <dbReference type="OrthoDB" id="311092at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007307">
        <property type="entry name" value="Ltv1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21531">
        <property type="entry name" value="LOW-TEMPERATURE VIABILITY PROTEIN LTV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21531:SF0">
        <property type="entry name" value="PROTEIN LTV1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="227"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="476"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="227"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="250"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="271"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="306"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8164262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="579" mass="68693" checksum="BA4E832E82F8067E" modified="2022-10-12" version="1">MGKKKFINKQNAATLKMVHQPGDNNQGATDMICFQKVNQPGAQNDELSELIEKELNKGKFDPNLKPRIPQIDPTQEALHLVAGREDLWIHQLAKQKALQALKEKQEQFQDVVMKHYDENGLPLDGYDYSQHLAPERPQGEVECVFVVPEEERSKVVYDIDFKREELTKEQQEVYDLLMKEEEEEEDQNNQDNQEELDDDFVANLIQQNKQQSFQQENEEKVEKIEKVEKIEKVEESPKKQVRFQDQVESDHDEDYEDEEEMEITYDKKQMNQKSQQQKQQQKQQQQQKQTKQQQSDSDSELEIDSDMEREFEREMNAYEAPDDEDDDQQNKDFVNDQMLNKVIDDHLNTMTKKQQKKSKNQQKSQDQPEETEQQRERRLEYLKNTDYNEYLRQKFPNADFSQFHLKKEIPPLTPEEDIALQNYLNRESSDEECPDMEPAEKYQETIVTKSLHNTTMSKMNPDQLNIIKYKSKNQVSNTKEDRMKQKEEEKNKIKELKLEKEKVKEAVCETLKQVVIKKEETLEEKKHRKELVKQLKQAQKEKKKAFKEQLQTMSKKLQKQKLANIQSNQAEGISIVKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UKM7</accession>
    <name>A0A8S1UKM7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0410179</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UKM7"/>
    <dbReference type="OrthoDB" id="306056at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="182"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8164723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="378" mass="43939" checksum="F23892A9A209175C" modified="2022-10-12" version="1">MELKQLNKFTQEYNLKKSDKLIKYLIILGIGVVKQQLNDINEDGIKELSNAYKEKSIQQEIKSLRKKVLSLEKHFQPEQQIKYHSQPKLLPSKITPKITEQIPIQQNVKKVIPFKHHLDDSEIKSILWNQYQNNDSQHQPKRKQTLDQQPIIPQYLPRNDSQNEIQPLSQRENSARQQTQRSSYHSSQLDSLHSLHSRNSPQFKDENKKIPKENKTPQSNGVPKHLRQVQSKIKQQILQDKEQYKSNHIQKIDDQSINIVNNSLNPFNNTPQNKLFQPCQSTHFGSGQVCSAQQQQSQPQDSNTITERKLFNIDEIASSFLNSPFLKSQIKQKLFNKQESASKSSASSTFSLFNPNNELKNFFQQLDKQVGNSLSFQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UMA7</accession>
    <name>A0A8S1UMA7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0390315</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UMA7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8163626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="146" mass="17816" checksum="A4FBD4A7CA26614B" modified="2022-10-12" version="1">MKMGKSKDNGKKLKFFRLDNMQIIKGQDFGDIRIKLKYIQVLIILIRIKVTYLGEQNKDKKSRRYEKIYYQLSRGDQYDRMGLKMIDNWVDLFENFQKESQITFSGCMQMVNNREDGVFILEIRVTFNLNQFNVFYINYFKWMWNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UMY2</accession>
    <name>A0A8S1UMY2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0440129</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UMY2"/>
    <dbReference type="OrthoDB" id="306099at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000089">
        <property type="entry name" value="Cyclin, N-terminal domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000283">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin N-terminal" evidence="1">
        <location>
            <begin position="128"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="2">
        <location>
            <begin position="245"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8166400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="369" mass="43334" checksum="F7AD9FE1F5EDB430" modified="2022-10-12" version="1">MFARRNGSRLKLNETQKNFVIKRACSTNKSSISSLNTSNSSKFFQFEDKTHPEDGKLLDLLSENENLQKSQIYHLQMSSFQIHNDESVQQRYALSDDVNNPQLRRNITYNWHYHHDHQQYLCQDTVSFSFSIKKHGIKEMLRAKMIDWMIEVFGNYPTTTTSQTYFRAVGLLDAFLKKTSMYYYDGDVHLMGISCMFIASKLEDIYHIPLNDIVTRVGHNKFNSLKVKNMEQTILETLQFNVYFPTPLDYLQNLFYKCFSLNDNQTLQNIYETCIYILKMCLHDLHMLNFTPNLLSAAIVGYAVREYIDTKQENKKADNLKLNKQSQDSIVKIAKIDFNTYNECQKQITELINTFKSKYPDLNNLEKFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UQ93</accession>
    <name>A0A8S1UQ93_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0430199</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8165932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UQ93"/>
    <dbReference type="OrthoDB" id="293557at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052981">
        <property type="entry name" value="Ingression_C2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47052">
        <property type="entry name" value="CONSERVED SERINE PROLINE-RICH PROTEIN (AFU_ORTHOLOGUE AFUA_2G01790)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47052:SF3">
        <property type="entry name" value="INGRESSION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="318"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="340"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="103"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="59"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8165932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="409" mass="48225" checksum="BDEA047512A36B7A" modified="2022-10-12" version="1">MNQQQYNEMFTAFQTNKILVEQFDIYLKQKCGHFYDEHLFQQLIELSLNNGVIDLECFINQYNETVDTLQYRSEILNNKIKELKVLNQQLSDQLNSNALQQNSLTIQIMDCQNIEIRDPSVYLQICCGAKTHKTRIIKGVSPQFQEQFEFPIDSGCQDIIITLFDSMNENMIGYTQFLISELREQKLKDISVNLQRQDNMKIKTKLHVILKFNHSKESLYKQEQQKLNHEINQNNIEMKETQQALNCLAKVFSGGCIKIRQLPPILEIKEFELKCLDTLNDALQERIQDWVPLFYKLTGLVVALSVMISLYRCQFMELTLLAIMMQLLILRDINQKWMRIFTYLFAVLFLYDLLWLYICGGSYGITKGEQHINKFVTALSYIELLTKIPFTIVLWKHSVEFEQFLSYFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UQH9</accession>
    <name>A0A8S1UQH9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0470074</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000047">
        <property type="protein sequence ID" value="CAD8167350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UQH9"/>
    <dbReference type="OrthoDB" id="297091at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8167350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="128" mass="15282" checksum="A474EA6BE04B869D" modified="2022-10-12" version="1">MLDNCDSIRKGLLYTPTMGEHIEDEVVVSFEQKRGINYQHEYMRLYIANVVLTQQLKELLQEKGDLISKINRFEETEIVKTNPRKQDYEESKKIEQPSKKQIEGQIKYNDENVVHNHQYQTRKLIKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UR73</accession>
    <name>A0A8S1UR73_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Calpain catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0460244</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8167131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="167576at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053033">
        <property type="entry name" value="Androglobin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46298">
        <property type="entry name" value="ANDROGLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46298:SF1">
        <property type="entry name" value="ANDROGLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calpain catalytic" evidence="4">
        <location>
            <begin position="222"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="409"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1139"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="520"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="1275"/>
            <end position="1302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="423"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8167131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1418" mass="165984" checksum="BB3BEFC1620C6098" modified="2022-10-12" version="1">MPPKTTKDQKGRQLFRVGTPLKDVIPQSVKNPPRDPKPLKGPEQINKQSMQEYHPFPDPEEWPGDEVVQAINLLQGDAQLTKCNFETDKYVPFWRRPKNFLRQRWQQESEHEPPYMEMRENRYNMNDDYYQTRLETIEKIHFMEKEVEHQLTICESYERMETSEEVELRRKEFLEKQAAQQKNKKGNKKQAEEVFDENPKMTSDVRLSDLICNDTLPPNSRWIASQLQQIKDRDIKDCFTQKSLSSKIYPQKDGAPIYNPNGKYIVKLYFMGKERKIQVSDHMPTTYDGKALLPQSVDKNQLWPMIISKAILQLWDYQSKGSLVGDGFVMYSLMGLLTETIDLKTISGWGVIDNMMNNNHYVQKDVFVSTYSYKNQSNLNTNNRSKKEYLLELLRNPKKEYDNYEIARNKNSSPSQHQQIQQEEQDEQQEEMQQEEGEQQIQRFRKSQRGFSFESLSRSYSIPRPSIPPGNTNLNTCFSYSIVEQFQNENGFNMVYAQKRSDRELRLRQEYFDLCKTPMNKMTKEEKLELRRRKKEIKEKLQDDDKKRISLISQQPVKHRYLRLKSDVQGKDPVIVFSPLAADEIYIAKKCIANKLNKPPNYDIPEIKGDDKSVVSANMKNTDDSFTVKPLEDFSSVNNAQEPFNRGEGGFWVHEKEFLSLFDYIQIAYDPKRYNLQILQIQSNPDNDIASYDNIEMIIVQRDPDSDDNAQVAFLYGFQPKYSQDKDLNEQIRPYSMLQRFDLETYESILDYKLLNQCVSSQMLLLDNKNHIFKMNIYSPLNFTIWFASNNKINTQSVFDYLIDYENYQKQSFQIDYPSCEANRYFVYFRFKVFLNSNKENGSFVYRLKSTTDNQLLKFLKVKLCEIPPPDNNLMLTTIEGVHKNLFESESIINGTQRMMLRSQTTYYFILEGTPQYNTQEGTFELDFLINQEFTFTQLENVEPARYYDKYVPTKYGTIFRERIFANEAQASIYVRLTEGQQAQQQQQVAKGKQTKGGAQELEIVESEMKGERLIRLELFHGDDLIVYNYGINSVTLSNITIPKDENYVIQASFDLREWPEAKIKSEETDNLYWFTTIFASDTVALVRDTTKEDKEKAIKKSWEDKEPGRAANAKKSRTKYLITLKEELTPEEQAIVNAPRMTKKQREEEAKQAAQKKGPKKDDKKVGKQTKVEAPVEIVAQQRQIPKSENHVNEAITQFLQHLEQDRIMDHYARHAGLINVRSDVQKREILEGILMGKEEIAGIIQRNLKMREEIKLLQKDNKEILINEFQSYRSSYKQELAEIYQQRDAIKQDLTQLMKKEQQLFDLCKQEKVGNPEDVEKLIADPSQLDPVLVSAAKQVINNWKIAIIQEKINNALQNFDIDTLQKCVDQIQQLNIEGIDTSAADDMLDEASGNPNFQAEKLAELKKQGKKPNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UV95</accession>
    <name>A0A8S1UV95_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0480221</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000048">
        <property type="protein sequence ID" value="CAD8168167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UV95"/>
    <dbReference type="OrthoDB" id="297743at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8168167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="327" mass="39158" checksum="1E97A4C276FC2BF9" modified="2022-10-12" version="1">MNKKLANTYNQISDVFSRAQIKNAVESLDNYHDRLKLIDSIIDRRQQIRQANDLSKITKYAHIFRKMIQRYRNKKEDQAANQRLQMQMVMSMFAPKGKQSSPRHLYKNLEQRKIDPQQLKIKEISEQRRDAVQFKMNRTSKISNFHSDRKYNNQRNNTKEGCRFSNRTQRFLSSIDVDDQYQEKELYLWSVDDKEIKSLQSKIKQSVILKTRINLNQNTKDNENVLKLLQKQQSTLSRRQYKPYSQPQLQDLGDKEQDIQKYDQMQNLISQEGSYLSYSENRLNQLYKKTFQIQTNSFQSQHNQQKLIKTNKEIIEISKIKLAPQKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UZE4</accession>
    <name>A0A8S1UZE4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0510171</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000051">
        <property type="protein sequence ID" value="CAD8169637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UZE4"/>
    <dbReference type="OrthoDB" id="307637at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="247"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8169637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="522" mass="60965" checksum="79B45949D58181C1" modified="2022-10-12" version="1">MKKCDNERIAQQIYASQMLKLQGKILEKKLIKTGSKNQIESQSQNISNNNSPVVGRQKSYENLQKVNKTFINQPVSQQKLSSILNVISGQQQNTKQKYSINNELIETVKISHKLRKNQSMIHIEQQSPALTKNASFHHSVKNKINSNSVSSDQSQIQKVKEKIKLLLQERDQNLPQDAKELAFMTKFNQILNQLVVVLFQEPNLQASQQQFNLPSSTQDTFQAIQIDHFFKRQIQILQQSFQLQLDKKKLENVLLQIKKKQEILQQENDQLSQNKLQMNDQITQLKQQITELQKQNDNNEENIQLESETQELKYLVQGQFEAIQKLLQREQLMKVFLKRVGDSSIIEMFEQFIQSAENVNQDDTEGNLNIDMLPQQKHTQVQKNNQQQQQQQIPDKIIQQYEISYKQLDLCDSLLADDSRVNDSEESSFGYLGKEQATEVSCYFTQAQQFQQQQNQQPFQQQQNLKVKGKSNIKDKLRINMMDVQLAQAAQKHEYLKQQQLLQQQQQHHDKILPDDINSEDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V068</accession>
    <name>A0A8S1V068_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0500195</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8169169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V068"/>
    <dbReference type="OrthoDB" id="287711at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="446"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="616"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="448"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8169169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="750" mass="88133" checksum="7E40AB47ABCD5A2F" modified="2022-10-12" version="1">MKSNHPYTITFPIWQCEQWSALKGRLKLTDEQVVIARWNDTRFLDEELAFLESYHRIKPHAETKLLKPQIGLLPSILQIVRTNTYTDLIIENVSQWLLINGDLSKLLLIYFGYDLYQGFSPRDCTIENIVKNGDSIVILDFGLKKRQENYCVYWNPLILKGKPCRSSYQWSLGILYLVMTQGTYILNEVHRHISDWLQGGRLDIGSLISNKKPTVQNLITSLLNPENPIPWHQIPYHSAFREDNACKQILKDFQIRLNRMEVKCRSSSRISSRDDSNSLINLVQQPSCNKNQKISEQNLLNQSLITKISNMQKMHQENVRNVVGNRYQSVQSRQKSSSHQSRQMIQTYSSNKFNNFFSSTKLLPKPQIKSQNNSNTGVKSFRNQTQNTTIYTGYDSSRKINDNSQELIHQQGSQKQISQSFTKNEFFYDKIRARISSVQGNTIKMQAKQRFSSSSQNQKSDQNRQQQVVYSKESKNQDELQQLYKQSENAMEQYKAIYSSDLEDNQQQKHNLDESRISNAENYIQLIKRSTQQLSLFSQKYNRSLDALNIIGQTVAKCLTFFNGLQNFWVIPLFLVFKRMLQLRKEIEILLESKVNSFGLDEWDYITSCFEFQNYCNKVKQDNQQVQNELNLLLNTAKAKADKLDKNRKDKVEWFLNDFIDDDIKDICYTYFHGQIYQNIKDKKGLPKQTLEWLRLQIQAQASLIIFELPILMYDKENFTYEGYLQAMEYTDENQILQYLKRNEQYLESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V234</accession>
    <name>A0A8S1V234_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0550010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V234"/>
    <dbReference type="OrthoDB" id="308377at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="54"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8171410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="247" mass="30008" checksum="68DDE13C7ED347C0" modified="2022-10-12" version="1">MLKKEEIWQSIKFFQKQNRRELDQTRESEDQSSFISDNEKENLFKNKQQSCQCKQQVLQLKKEFMLQIRELKAQHQIEIENLQNQFQKLMIEQKQKYEQEISQLKEDLEFYMCEQNDKELLELIEQEQQKDKEIFQQQLEYQITEKLKLEQKLIQCQRELKQYQEQTQQILSSQNTQQNYQQLQQSVHKNCAPQLFRDTNRKVKLQTEGIDRSIVNDDNSNKVGICNSIKNFNANIKHMEKSQQSQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V3D7</accession>
    <name>A0A8S1V3D7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0540125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8171127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V3D7"/>
    <dbReference type="OrthoDB" id="293435at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007246">
        <property type="entry name" value="Gaa1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13304">
        <property type="entry name" value="GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR ATTACHMENT 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13304:SF0">
        <property type="entry name" value="GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR ATTACHMENT 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04114">
        <property type="entry name" value="Gaa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="418"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="452"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="514"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="544"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="585"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8171127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="607" mass="71973" checksum="392A428BAD382C80" modified="2022-10-12" version="1">MFKDLGYQLLIKWKLWKVLIIIFPFTFIYGIYLLFVVSSNSDRNSFDGSQLIHVEVNKQLSQTLSLAVEQYMKEFSNTSDIHQLLMQKLSNLGLETFYQQKYNNVIAKLKAPRSPDYECNLISFEYNDESPNIKIRSIAYVLSLIKLFQSDQINYLSRDLIFVGYHSKYKRYGQGITAFLEEYVNPQEVSFMPRSGTIRSSININLDDKFDSIALKVFGLNGKVSDRDYYNSINTIMERQNFYYQFTETNYYWMQQFEKYLEKEIRTYWKLLQLDSIYQIPQKRLLYMDPYFSNQIEMFQGDLHEAHSYIMKYGIYSLTLRGYNTQNKDDSNILTNKLLQVGEASIKALMANDEFIHSGSTLYIPLNKSSTLTIQNYCLPYVLVILSCAIFAIKSVYSRWHFNLFNDDKNELGRCNSMVAHKSIIEIFMGFTLCFIPNIISYIDNQQFDYFNQYFTIMLVIIVIYLMIIYTTRRWIFGQYTLPRKESVAEWLWVELVIQVCFVMMYAGIVQIPIATFIAIFLGPWFFFIQPLRFKPNFKFLDIFSYVINLLFLYGYFQILLYFGFDSMFTYYKEVVVNYNKVGIYLFYYLNGGLIPSLIRFAELLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V3S4</accession>
    <name>A0A8S1V3S4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0550084</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V3S4"/>
    <dbReference type="OrthoDB" id="304122at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF274">
        <property type="entry name" value="MYB-LIKE DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="4"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="2">
        <location>
            <begin position="4"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="2">
        <location>
            <begin position="64"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="64"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8171555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="354" mass="41639" checksum="6BD8706B7B34C804" modified="2022-10-12" version="1">MQSKQSETRQSWSYQEDKLLLELIKLHGCTSWNHIARELQSLGKNPLKVRSSAACRERYQNILNPDLNKSNWTQEEEENLFNLQQSCGNSWARIASQMPGRSDLLCKNYFYATLRKVLRRLSKAVGLDQSSDLLKQVKPSVLGVIYSKDDSFKRFNIDEKMKTEFQQLIKQYRYTEKAELKQLQENDVVYIKSMLNQLFVINNNYITQMKRYSSRKNSQQEVFFVENNSKSTIPNCVTFQNQKITKLECKLLNPTNLKIDSQQEFDQPKSNLSNVQQENQSIQVYQNLNQLHSDFSPINQFQGSFCAPQPVFTFCIQPNFISQPIQSIQPYYIPQYLPYPQPHPLIRYEYVQMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V3X0</accession>
    <name>A0A8S1V3X0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0550127</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V3X0"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8171641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="216" mass="25670" checksum="8B439C965604ED2F" modified="2022-10-12" version="1">MASFKRQLNMYGFEFSRTSEGLMKFQHNQFDSKNNEGLQMKQNSTKNSLTPDQELYLLKLQVKDIQRNQQIMQSQIRQIIDRQQEIQKNIKKAMLDYVRIRVNGQTKGKKVGKFVWKFHLNITNKKYKRFLYQKWKEANSFQECPCIGDISQQTFKQQFYTYPLINNSIFDIKIPNLNLRSPEILELCDTQDQNESSDLDLMEVQSVSNISNYLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V7D6</accession>
    <name>A0A8S1V7D6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zinc-finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0530200</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V7D6"/>
    <dbReference type="OrthoDB" id="308391at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018866">
        <property type="entry name" value="Znf-4CXXC_R1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10497">
        <property type="entry name" value="zf-4CXXC_R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc-finger" evidence="9">
        <location>
            <begin position="132"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="64"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8170696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="318" mass="38354" checksum="741BCC02723027E2" modified="2022-10-12" version="1">MHTQNNFIYNQQGYMYPQQYMYPHPPPNYVFPQQIQPAQFYYMNPQNPAMYQYNIGQQQQQQQQQQQQQQQQRIKCNQPKTKEQDIKQQKQQVVKQQNNPIMQQQMEMQKTNIKILSEQQIRDQVGSITKQGFCHYCNKNGKMCYYCFSNYKSTDCRHGFCFDCLMFKFKINPIKILLRPDWSCPIKSKQCMCSRCSNINHMDSDQFISNDSVESCNKYAHIIENTKKIKKQYSKKKNIIREMFQVKFQQLDPKKKKQQQSMKKTLLLNKIKKQMQFSQECILKLKQKHSQNDEQILHQVVRYNFGSLIDLFKEYKQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V7W3</accession>
    <name>A0A8S1V7W3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ras-related protein Rab-21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0560061</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endomembrane system</location>
            <topology evidence="10">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000056">
        <property type="protein sequence ID" value="CAD8172052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V7W3"/>
    <dbReference type="OrthoDB" id="63533at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032482">
        <property type="term" value="P:Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04123">
        <property type="entry name" value="Rab21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000808">
        <property type="entry name" value="Small GTP-binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041833">
        <property type="entry name" value="Rab21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8172052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="201" mass="23077" checksum="4DC783AF2CA44E90" modified="2022-10-12" version="1">MKKQKFEDRVYKVVTLGEGRVGKTSIITRFFHNSFQEQTQETSDGYCKEKTIQTKKGPIDLAIWDTAGQEKYHSLAPLYYRNSDAAIIVYDITVKETLQKGRQWIQELKQLAENDNMLLVIVGNKCDMHIHKDVEQSQIDELCNQYNAKHFLVSAKSGKGITEVFDYLANELMQFVKQTKERSSLQQGKKKQQQNDGGGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VAV5</accession>
    <name>A0A8S1VAV5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0600005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000060">
        <property type="protein sequence ID" value="CAD8173685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VAV5"/>
    <dbReference type="OrthoDB" id="291524at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8173685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="755" mass="89040" checksum="6B2F72ACBE252B85" modified="2022-10-12" version="1">MSKNVNEEFKEELRQFIINNYVHTVLEQFKGPKHQSIYQITLTMDLSLIIDTRPLLGSWIINFYPHFLISIHQIIYSIVKQMDIGYDDFICETKFISVPTFETAIHDPQYKLLLMQKHCGSLVHLKNCIIVGVDKPKNYLFLQEFQCKCSDKISIKGAHLIQRLQNQMNKENVCEECNGVRHEIEKKYKLCQKLQLYVSDLQPLNRKYNNLRQNLNNKYKHSFQLLDIYVEGNNVNKFNVGNQIYSLAYYTFNLSDQYRQNNTFILANEYCYAVTIDQYSAVQNKKYFQKLKLNYKFKQSQQIDTDENYNEDIKLFLDKSKRHYNYGKDNKNYIYEILNTIYYIIISFDYQQLKQHTLSNSLIMLKLSLLCSIFLENSSSLQYYLSPNLCQKSNLLIQRLEQEQIQRHLHIAIIYEDYEIIIDLIQNMIQNLDNCLVLPDCDEETFDYLIMSVAKNGILILKRMPTKGVQEKIKQILKGSPIIIMKDKKIEINCSIWCLFDIQDLKVKKSDFTYLNEKFPHATDSFDIVLDLAYSNLNYKQVRFNLIADYCEYLFKSSQTDNSNPFSTTIKKQPTADRSIHAPSSCYLLQKRTVDKIYNSDLETWINKLLTIDSSSLELIKKFYLKLRQNYYCTQHTLDSLLKISSSISMIRYFAYSQIDDNDGLYQYMITQMSTLPLTYSDAFLAILFVIETRINSLGPVGALFGSDTTNYLLNCLNPEFAFAKINLHQDDNETKDNMFENFVYIYEKLINFIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VE01</accession>
    <name>A0A8S1VE01_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein kinase-like domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0610228</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VE01"/>
    <dbReference type="OrthoDB" id="167809at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000737">
        <property type="entry name" value="Protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="10"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="292"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="328"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="531"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="567"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="404"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="626"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="634"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="694"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="706"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="729"/>
            <end position="743"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8174683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="873" mass="102496" checksum="89BCDF392247EE9E" modified="2022-10-12" version="1">MSRNIGDFTFNSNECIGQGTLGKLHKGTNKKNNQDVVLRLLQKSLLKDDNIMSAALTEEMALMQKLKHPKINPIIEVIESQTFFYVVCPYRQSNLQTILQKQRKLPEAETIKLINDLIDGFSELIKQGIIHRDLRPSNILYDNGSYIISDPGFLKTIESFKKDNKTKPQQFYYSAPQVLENKSKYSNKCDIWSLGVIIYECLTGVLPFPTSSLDEYLKSCRSKKVEFQQGSISGGMKSFIEGCLVYQEEKRFDWTKVLSHPIINAKLQNKNQDIVKDNKKYLMTQIRQKIIKKNLDLQDVYQKIDANHNGLLDFDEFTTFISMIDKKVTKEDCYYIFQQIDKDGSNSISFDEFKLWISSNNTRMAFFKINKPDNLQNNKLTQQPKNINQQQEFIFQQQQNQIKQQTPYQQQQQMNPFQSPLNQQQQQQQQQQQQQQQQQQQQLIQQQPINYQQQQYQQQPIGYQQPQNQFPINQNLPLQQQFYPQPQPQPQTQVFPQTQMKSSGSIPAGAGFGRSDKLERTILMIRNVIKSKQMDVLTLFQQFDFDKDNFLNFQEFSQLIWSLNNQLTVEEIADLFKIFNLNSDEYVSFVEFRLVASMANDKKQNVQYLPMYQPIQQNNNFQQFNPPFQGYQIPYQNPAQSPNIPMIGNNQQPIQNNYSMNKDQYFTPQPYQNNYPTTQLGNNNNNKNIKKQNDASQLDILSTKQAKKDNKKKEREEREAKRKQQHSFSPKEEKPKKHLEEPLRPPMLTKQDKQQSILETPKYPTEKIGSQNKIPSPHPSPRQDKRKVEQPTVTYAQPKNQEENQNQQKASPLTWYQPKYMTDYNYNTIGSKLDQELAESTNLIQNSSIPNFNNKPYRKVLDDKIDKFYYSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VER9</accession>
    <name>A0A8S1VER9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0630181</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VER9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8175680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="264" mass="31806" checksum="30ACB3AD27E76860" modified="2022-10-12" version="1">MIKILKFYQTLAILLQLSFSTNSSNIIVSLNQFFAFTTISIIIFQLYQQYNILNIHQILANNQQFSDQQGMYKLKKRSFYYFIKILILKINMDFIINFLDQQVKFFIFFKWLIIIILLLSKQLYVLDLVHQEQFFYFIKIHMKKKKIFYLNMISNLSISSIFFIVIVFAINDQKNIKFIDKNKDVFGWLIISLVLLAIFVELCSLTIELILHFYFIFKKIKKKFLKYFIKTNTINQSQKNETIQKTVNCSNIKKYQSYFQIVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VK35</accession>
    <name>A0A8S1VK35_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vacuolar protein 14 C-terminal Fig4-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0680016</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000068">
        <property type="protein sequence ID" value="CAD8177654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VK35"/>
    <dbReference type="OrthoDB" id="5574975at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070772">
        <property type="term" value="C:PAS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006661">
        <property type="term" value="P:phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001191">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026825">
        <property type="entry name" value="Vac14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021841">
        <property type="entry name" value="VAC14_Fig4p-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16023:SF0">
        <property type="entry name" value="PROTEIN VAC14 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16023">
        <property type="entry name" value="TAX1 BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12755">
        <property type="entry name" value="Vac14_Fab1_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11916">
        <property type="entry name" value="Vac14_Fig4_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Vacuolar protein 14 C-terminal Fig4-binding" evidence="5">
        <location>
            <begin position="500"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="374"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="551"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF11916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8177654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="733" mass="85694" checksum="933EC4C9D630B774" modified="2022-10-12" version="1">MDDNVVPQQIIKLLYDKSQDKKQQGCQQLKSLVQSYAQSGKELLIKQIIETLKIKFTSSSQLYLKRQGMFAIQTVAEILVKEFPQLAERCVKDLTQPILECLNDKEDKARQYAVECLLQLTKIMKTMILLNFNEIFDYQLGRSAEQDNQIVQAMFLLDAQLKTQVQIAVQERFSDPGKPCYFNLNSFMIQLQSKLKSRVTYVRQFLLGWIKVLNQCHNNDLYIYFPMILEGIFMMLGESNKEVRNGADLQANEFLKQVEIKVNLDQRVNEQIIEILLKICQMKGNQNNYAKLNSLLWIFEYLRVFQQELEEEQKGKLGFQHSTSHIKQEEYLRSNTSPISKNQMQQFLGGFESPLRKTILNSLSQILGPILILLSHEEEEIRKAAQKTNELLLKVMERVKNQSVEFMNIVPTIKEMLTDKKSNTAESALIWMKHLLETYSESLFPTIEDILTKLIDKLADSESAIVQNIMDVLANISMHSQYFEIVIDKILTMLHKNQEQSEKKGDQIFKKLCSLLNPQKVYFTITAKLLEIYSDNDVLFISNTVQTLTTLLISEKELQNLRNILRNLKHEKDEKQRQHNQEIFETLYRTFCYNSMSVITLCLLSEEYELAYNIIISFSEVEVNQYILLEIAQLINVLETPVFIFLRLQLLEYDSHPFLMKCLFGLMMLLPQGPAFNMLRQRLKNVSNTQIQSLQQDKKMETEEFLDIQKLLTQFRDIRLQCINQESKQKDLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VK70</accession>
    <name>A0A8S1VK70_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2</fullName>
            <shortName evidence="8">Oligosaccharyl transferase subunit OST2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0690143</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Endoplasmic reticulum membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the DAD/OST2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VK70"/>
    <dbReference type="OrthoDB" id="445566at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003038">
        <property type="entry name" value="DAD/Ost2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10705">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT DAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10705:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT DAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02109">
        <property type="entry name" value="DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005588">
        <property type="entry name" value="DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="100"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8178338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="118" mass="13458" checksum="974A7F75AFC6BDF6" modified="2022-10-12" version="1">MSETKFVDENSLIGIAASFWQQYSTKTPQKLKIMDAFSLYCFILVIIQLFYCTLVGDFPRNSFLSGIFAAAGAMIINICLRKQLNPETRYMAISNERAFWEYLAAMVVLFLTVINFLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VP03</accession>
    <name>A0A8S1VP03_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0680182</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000068">
        <property type="protein sequence ID" value="CAD8177935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VP03"/>
    <dbReference type="OrthoDB" id="306248at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8177935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="239" mass="28142" checksum="4D29C212FAC8BDAE" modified="2022-10-12" version="1">MFKKNNKENLKPITLNIPKMILKNQINQNSIQQYAYLIETSRSPRISQLYQKIQQSIDQKAADESQDVVSIHQQKIREKIRGYNQTPTITTSQDIQDSFNIWNDEQQLDGNIIDNYTQRYKSYDNFTQNNVSQNEIAENILKLLGMKHKVKNLGKIPFLNDEPKKTTNYFVPPQKAWRLYKKRPLNFTSTLYTSNQTTQLINSKSRLNSLDIQNQNQSNLRQLSSPDILRIKFKQLKMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VPS6</accession>
    <name>A0A8S1VPS6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0650177</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000065">
        <property type="protein sequence ID" value="CAD8176626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VPS6"/>
    <dbReference type="OrthoDB" id="313544at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="198"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="397"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8176626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="445" mass="53274" checksum="383BE391CF9F2819" modified="2022-10-12" version="1">MIIFVYYNKKKIKIVLSQPSLQLLYKFVQENLKLPFDFELRDNMNKIVFDLYDGLELNVCEVITINQQMKSKTVNDQQSFRQGRERIHSLNLLSSQKKSYSDFSSPNDQTQIQQNKREQIIQKMMEDKLYREEQQKLNNEGNKLIKQIQKRIKLREQVIKLKDEALNLSELEKKKIILAQKRELSQPIRLDMLTEHQKKYEEEKFQKLQQRQQQKQEQEEEFKMKLIKFPKSQTLVRLEEEQAKLKLSQQQAAEQKKLLKQKQLRYGESVKDSFLKDIARHSISPIKQTQEQNSIQKTQLDIAKQNIKKLQSLLGEKKVDKILILPERRSVNDIRQRGQNSLIEMKRSDKIEHLDKPYTTDHKNLISKRKMLKSGLNSANFPSTLNDNKPIHFEEKYQNVLKSIMELEQKCKEREKRFMLNKSQQLEEEENEKYIEKLRQKLALI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VRP5</accession>
    <name>A0A8S1VRP5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0740034</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000074">
        <property type="protein sequence ID" value="CAD8180294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VRP5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8180294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="234" mass="27507" checksum="3939E8B1E9EB62FB" modified="2022-10-12" version="1">MLKIEEYVCNLKESKTLFNDALRIDTHQSLESYDNTQFQNPIYNIVPPEYFQSVTENKYSNIFSSYIQSPKHEELQSTIKQTPKKRTFSFAESQYLNKDPACNISADFGQNTQLPALSELKFYSKFSQENNFKNIDTPNKERWQLQDSMLVLNNSIPQSKFSSPQKKYDKVQKFKITNYSLDAKQTQTTKITQVTNISLQDNNKEFEQIFKECQNNFEKNKARKTEEYIACNIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VSH0</accession>
    <name>A0A8S1VSH0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0710128</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000071">
        <property type="protein sequence ID" value="CAD8179203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VSH0"/>
    <dbReference type="OrthoDB" id="342730at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016235">
        <property type="term" value="C:aggresome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005164">
        <property type="term" value="F:tumor necrosis factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070842">
        <property type="term" value="P:aggresome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051865">
        <property type="term" value="P:protein autoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053003">
        <property type="entry name" value="TRIM_RBCC_E3_ubiq-ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36754">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36754:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="3">
        <location>
            <begin position="63"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8179203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="368" mass="43183" checksum="01074C24535743CF" modified="2022-10-12" version="1">MDSAPKKKKSVSSKQDKSQAGSVKSKKQQPLGEILEQQNSQHDIQIDDHPQQADPFISQPPQQQIKVCIHHNLPFQFFCEACEDPICQQCTILGPHNTQLHRINRLSDSFKLRCAKIEESIKANLLGKREQLLAQIHRIEYRIEEIKYVKTIIERDARAEFGGVLERLKQSEGQKLAILSHEISELQRDLDKINEVGSQFYDLKEIPDPCPFLLKSRNIYDQIEYMVAKPFKVQIEVYPYDLPRELTELRLQLQRASAQQQLIEFKNEIIWKLLSEKQIDEEAIKQELERQASEEINEWSKLVDKFSMELRKQQLVCYYCGCKMEKQYVNSICVQNRQEQDYDKVFCNEQPPQNHHGTSRHYFADPIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VZA4</accession>
    <name>A0A8S1VZA4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0750215</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000075">
        <property type="protein sequence ID" value="CAD8181129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VZA4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8181129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="62" mass="7590" checksum="094A6A2BC4E46061" modified="2022-10-12" version="1">MKNSTKKLSLYFNKLSYQLIRREIERNIQRSTIRGIQVQILMIEHKLIYQGLNFQMWNSNEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W4G8</accession>
    <name>A0A8S1W4G8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0810063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000081">
        <property type="protein sequence ID" value="CAD8183567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W4G8"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001886">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000683">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="103"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8183567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="446" mass="51726" checksum="1F4A331082F82DA1" modified="2022-10-12" version="1">MLSYENALFTVQVTRKHFFKDKNYSLYLFSEELVMTDDITKQPKYNLKMNLTTTIKWILEEKRIVGFEFSYNNGYKIVYGSKLNLLKEMIAGKIFYQPILSFYQFQMEMGSGMTGSVYRCVAADNANLYYAIKKIDKLKIAQHEGGIPQLVHELSLLSSLSHPNIVKLKETYTDNQYYYIIMEYINGRTLYSELSSRQYGLSIIETIKIMRELLEAVSYIHDKGVMHRDINPLNIMKADTVKLIDFGLARKIKNQLNFPTSGTPGYMAPEIINYNKDKQYDEKADIYSLGCLLYKLLTGENLFNPKSAKQSIYQVNKDGFFELKKQPQHPEVNSMKMEQLFILLPYMLEIDPSNRLSAKICLTIIEEIDNNNQNIDRLIKKILIRKSLNTTTIEEQRSCERISKGKIAKQSIDGISKKSLDELSIFTKKVMVQNQQKVILPHKKQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W7H6</accession>
    <name>A0A8S1W7H6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">asparagine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0770086</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.22" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000077">
        <property type="protein sequence ID" value="CAD8181816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W7H6"/>
    <dbReference type="OrthoDB" id="1931232at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004816">
        <property type="term" value="F:asparagine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006421">
        <property type="term" value="P:asparaginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04318">
        <property type="entry name" value="EcAsnRS_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000016">
        <property type="entry name" value="Asparagine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00534">
        <property type="entry name" value="Asn_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004522">
        <property type="entry name" value="Asn-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00457">
        <property type="entry name" value="asnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003037">
        <property type="entry name" value="PRK03932.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF34">
        <property type="entry name" value="ASPARAGINE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594">
        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="8">
        <location>
            <begin position="234"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8181816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="567" mass="64711" checksum="FB28A17C96815E8D" modified="2022-10-12" version="1">MQYQFLESDELFTRVKTITDFAKQTTAATQKQTKKTEKLIGEIEKPINVPPLPAGFKVDIPQFDVTKRLNKLPRSWDLPQQLYKKHLPKYNVGTIYRIQELLNVGEGYIGQTVTVAGWARTVRDQKNLCFIELNDGTSFGGLQIVVEDKLPNFDQVVKANTGFSLKVTGNIVKSPAKGQLIEMLVADPTKHEVVIVGQADPQEYPMAKGVQKPETLRQKAHLRPRGNFFSAVTRIRNNLAYATHVFFQNNGCLYIHTPIITGSDCEGAGEMFRISTIFDNDIKKIPQINGKVDTAQDFFKKEVNLTVSGQLQVEHFCISMSNVYTFGPTFRAEKAHTHRHLAEFWMIEPEFAFADLFDNMEAAEGYVKFCINYIIQNNMDDLKFLDNRVKPGLIDYLKDIVSKDFIRCSYTQGIDILLKAQKAGAKFENSDIKWGMDLNSEHERFIAEKVFQRPVFLYDYPKEIKAFYMKVTDDGKCVRAMDMLIPQVGELIGGSQREERYDVLAQRIKECGLKIEDYGPYMDLRKYGTVPHCGFGLGFERLVMMVTGVENIRDVIPFPRYHGSAEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W9V2</accession>
    <name>A0A8S1W9V2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0850241</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W9V2"/>
    <dbReference type="OrthoDB" id="421226at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="160"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="608"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="648"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="686"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="712"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="744"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="829"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="6">
        <location>
            <begin position="345"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="6">
        <location>
            <begin position="966"/>
            <end position="1039"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8185727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1164" mass="139114" checksum="E5B2227B5B4D92E1" modified="2022-10-12" version="1">MKQYINQLADKLQISTSDSVLIIWKVFINFCSFVIYIEISLELFNVEEFQKDNRGDIFFIWRIFILSLLIIDMIISLFIEYISNNGIIIQKPYKKVKYYFRHYFVFDLIVIVYLFVSTFQPLPFWVGFLVFFRIPSNLYSDKITEEYLIQFQFLLLCYRILKLLVTLLYIFDIFACSFYAMGLYSISISEPTWLEYSNSVIGNIDEMHHVTQYIISYFWAVETLVSMGYGEITPMNQYEIIIGDLALVGCMFVCLYVISTILSFQSDNSSYLDENMLSIKQFMIAKRISKKLYNEVSIFLSQFRIEQSKRDIVIEQVVIQRLPNQLKSKLYYEASLKIMKRILWIYNNFSNEFIEELSSFVREIYLNANTKISVIDDDDDPKIFVIEKGKVTVILNEKEINLLYRGSTFGNYSFFTQEKEQFITYKTKSIVILQYIKLTDFLKTISNHKEDHEKFYYILDQVLFEKKYSLIQYGCYICTSTNHLSENCQIISIKDQVYEEFYKNPLRNSIQMRKGFIRQNKKHSKWILNRNAIQITPEYPISFDIDKRYYSKLLFQWHPFSKHQQERMNVILKDLLNDSLKQDDLIIMSENKSKNKKKSSVFSSSNQYLFYWQLFMMFVNAVFFIAIPQIIFFWNYYQQFSDQGGFKFIRIIFIILFVSDFIVQLNTNFYHEGILVKNRFKIAENYLRNDLIFDLIPILVLFYISLSDKYVIIYLLCLIKLIPFYRFQVYLSDKLKIMPQLRQFYMICGMLIEILYINHFYGCMFYGGSVYMYTFHPNSKTWVNDPTMNFGVIITKSTSSKYLYSTYWAIDAITRIGYGDILPLNDLEFMITDLTVITAVAIVAVNISLIENMRKNSKLQNYFVDNLWIQQYLKKKNCSNQLKTQISKYLRYYHRIGQDRNIDIEQESLQLLPSSLKQQLMYEAYGIIFYKQHWLRDDDVLFELSNKVKEIYLGDMENIFLDNIPDQGQALYYVERGYVELFINTQYSSRKDVIIAQLKKDTFFGHYSFITGLPRKSSARTMQFASLIYILRKDFHDVLEMNKKFHHQFQALRDSIIYENQFQLIGLQCFTCNGNDHLSIDCPLISIKKQVLLRFYHTIREKSQSGLNLRKLYKQNLKQDRRFKKRKKVKKSKTLRIHDFEFFLDYTIPRTTINYVIDKDLHLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WAE5</accession>
    <name>A0A8S1WAE5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">H(+)-exporting diphosphatase</fullName>
            <ecNumber evidence="2">7.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0850147</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.3.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WAE5"/>
    <dbReference type="OrthoDB" id="5210at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009678">
        <property type="term" value="F:diphosphate hydrolysis-driven proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01129">
        <property type="entry name" value="PPase_energized_pump"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004131">
        <property type="entry name" value="PPase-energised_H-pump"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001953">
        <property type="entry name" value="PRK00733.2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001960">
        <property type="entry name" value="PRK00733.3-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01104">
        <property type="entry name" value="V_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31998">
        <property type="entry name" value="K(+)-INSENSITIVE PYROPHOSPHATE-ENERGIZED PROTON PUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03030">
        <property type="entry name" value="H_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001265">
        <property type="entry name" value="H+-PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="142"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="202"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="246"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="348"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="390"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="419"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="478"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="508"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="614"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="686"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="716"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="783"/>
            <end position="802"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8185552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="803" mass="87346" checksum="0059491E7A82C46D" modified="2022-10-12" version="1">MLPQKKPQYELLGVQNPENEQFIKFIQFDHLDIKGIIFLISPLGTCILLQILGFSYLIQFISTVLHISIITIIFATCILDQILRHDDGNKEMKKIALSIEEGAEGFFKAQYGTIFNLSLIFALLIFLVYWQKGSGELEKDEIPVGGLIIGLLEGGSFLFGAFCSGFAGFAGMWVSIKANSRVASAARTCYNKAIQLSFRGGYFAAVINIALAIFGISVLFLVIYFYCYITITDQQKLNEQIDKIPLLLIGYGFGASFVAMFAQLGGGIYTKAADVGADLIGKIENDIPEDDPRNPAVIADLVGDNVGDCAGQSADLFESITAEILSAMILGATLTHEANLHINYKVTFMLFPLVVHCLDIISSTIGMYFVRTQPGIPTKYIEDPLCIMKKGYRIAMIIGFFGFFFICHHCLNPLQHKDAWLYFGMCGLIGIIVSYLFIEVTQYYTDYHYEPVKRIAQASKTGHATNIIAGLSVGMESTGIPILIISVGVLGAYYLGEQSGIKNHQGELIGGLFGTAIATMGMFCTGVYILSMAAFGPIADNAGGIVEMSGCDEQVRQITDRLDAVGNVTKANAKGYSVGSASLASFLLFRAFIDEVNFLSPTKKILDIDITQPEIFISGLIGACTVFVFSSWALRAVGNAAQDVIKEVRRQFRENEGILQGTAQPNYKQCVEIVTKAGLREMIKPGLLSVLTPLILGITLRAINVVRMQELLPAKAICAFLMFSTCTGILQALFLNNAGGAWDNSKKYVETGELGGKGSETHKAAVTGDTVGDPCKDTAGPSIHILIKLYSTITIVMVPLFVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WAS8</accession>
    <name>A0A8S1WAS8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0870037</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000087">
        <property type="protein sequence ID" value="CAD8186370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WAS8"/>
    <dbReference type="OrthoDB" id="426293at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="230"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="288"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="337"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="367"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="7">
        <location>
            <begin position="474"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="684"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="684"/>
            <end position="696"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8186370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="844" mass="100083" checksum="FF343FF70CB7D9FE" modified="2022-10-12" version="1">MSVCSLSPRSQVDLKLFQQLSPQINDPSLQNLTLEHQQYQDNFKPFRKLPVLQKQEQIKNVGNDKITYENPICDDQIELPKSQPCQQKATRNIFQLIKINNLVKRFKNILLCRSYILTQSQKEKIKSNLIFQEKYTQDKKNIQKNTISFVIDPTNRFIILWDIIMFFVILNLIILIPFFWSFDQSYPILRIEEMSLFIIFFLIDIILKLNIAIIKKGNIIKTRKYILKKYLKTSFILDVLYLFLVYYTMKIESILIFISFCAFSLIKLQRVIGKIVKLLNLSEIQREIISLINLIITINLIAHFMACIWHYIGITTMEYEKNSWILHKNLQDDTKQIRYIFSYYWAIVTMITVGYGDITPQNHEEAFSCIFLMLLSCAVFTFSLNQVGTIVQNINKQKRQFQEMLRILTCYMQQHSVPDQLQSRARSYLEYRCIKRNQQSKYHLQSILEQLSSFLQQEIMLNVFKNLLQDCKIISHNFSEETIKKMSNSLQTAYYCPDEYIYHQNQLDDNYLYFLDYGKVEIYEQKSQQKMAELQKGKYFGEESFFTQQPHKFSVISRSFTKVFRISQTTFLNQLNKEENEVYHQIRHLFLFNSYILGQKCQICSQSDHQIDNCKLLNYKPDIEKLILKDNLKIQNRQKQKRCVKRSCKALQIQHQLKLVQKALEQSQGQDDILFDEDENVSLNPQELELNTNKDSPVSKRKESYPKQSEPSLKADIKIPHDSNKRIQFQKSDGFNKSKQFQTSQQLRQTSETEIKLFGAPAENRTQVGQNSISCNYQIVGFEKAMEFKKFFPQFNLHVLISDYNKKTKILNKTFKSIYQLFNHSIKKRTSVRKARSSKYLQLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WB37</accession>
    <name>A0A8S1WB37_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Palmitoyltransferase</fullName>
            <ecNumber evidence="8">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0880234</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8187274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="331948at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161:SF85">
        <property type="entry name" value="PALMITOYLTRANSFERASE HIP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="302"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="662"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="692"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="780"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="814"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="195"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="345"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="585"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="735"/>
            <end position="852"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8187274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="875" mass="101632" checksum="9AA74995F6F75065" modified="2022-10-12" version="1">MEESPATAEPLIQQPENKTIYNFMDITKVHSGSDLIEAAKKNKMPILNASVDTVPNIYDLEDSEKRTVLHYLALNSNSFIFNRLLIKFQSQAKEWLQKQDKYDLQAIHYFIYSGKIDMLDEISRYYIPKNLLGMAAIKNDVLTMILIREKLKEETFKNKYQDAVTPLHLACSTKSDYAAIVLMKWKHPINIQDANGNTPLHIAALNNSYKLIRKLIQRGGSINIRNKKDQLPIDLAEDYQTLDALKSFRFSWKSLLLQLNLKPRKRSYMQSLIFLLFFSITNFGTIFLVQNSTNKDQNTGSFIMFIIIVTLFFICFVLVSYSNPGYANPKDEQNLKSILNKLEPFEICYDCFIQTPDRSKHCEFCKRCVIKYDHHCPWVNNCVLYXIQCKIFFIAVVTLGNNVEPENKTIYNFMDITKVHSGSDLIEAAKKNKMPILNASVDTVPNIYDLEDSEKRTVLHYLALNSNSFIFNRLLIKFQSQAKEWLQKQDKYDLQAIHYFIYSGKIDMLDEISRYYIPKNLLGMAAIKNDVLTMILIREKLKEETFKNKYQDAVTPLHLACSTKSDYAAIVLMKWKHPINIQDANGNTPLHIAALNNSYKLIRKLIQRGGSINIRNKKDQLPIDLAEDYQTLDALKSFRFSWKSLLLQLNLKPRKRSYMQSLIFLLFFSITNFGTIFLVQNSTNKDQNTGSFIMFIIIVTLFFICFVLVSYSNPGYANPKDEQNLKSILNKLEPFEICYDCFIQTPDRSKHCEFCKRCVIKYDHHCPWVNNCVGNDNQSIFWLFLLFLFLDFLLIIILGILALTDQNTMEKNNFTILIILTIIEFLFMIPLLNLILTQIRNYYSGLTTYERLVLGKPSTKKRESIIQKMSIVQQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WFA3</accession>
    <name>A0A8S1WFA3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0900147</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8187993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WFA3"/>
    <dbReference type="OrthoDB" id="312651at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022617">
        <property type="entry name" value="Rad60/SUMO-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11976">
        <property type="entry name" value="Rad60-SLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="314"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="664"/>
            <end position="705"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8187993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="798" mass="93586" checksum="BCA1F653EDB80B23" modified="2022-10-12" version="1">MNLRIEIFGSKPQIFQVNKSSSVFEVIDIIIQKNKLKELNQQLSLYLNGIELKKETRIQNYVKFVNDTLQLKKNEQVRQLQGIKLVYNNEIKIAYVYLDDSNYVLYDQAEKQFSLSKDSFKLICKDQILEKLTYLKQNQIDANSTIVVKMLNQLNQPKQMINLKLIYQQQEKVLAIDIYSKIFELEEKVKSLFKINKEICILNDKKILLQDQKLIDVDFNQNFTLTILLKELYQKSLSYINIIIEYNGQKFQMQVSTDTLISEIIDLLQIQNNLTNIDLMMDSQMLPQNQTITQLNVKENSILNVVTKQNNTILILNLKIGSKLEKIEVEQETIIQELIQQIESNFQLQNIQLTLQNGQQLQNNQSLKQQNIQSGTQLIIQCFNPNQNKLQTNNDLINIQVSLQPSQQIISQQISYNSLIQDLQKTISNYLNCQNKIQLVYQNRILNQQSTFAQEGIPNGCQLQCNILIDKIDEKIKIIDKPVISDPLIKVLIIFQNQQKQMVLKGNTLVDTLINGVKSQYSLNQDIYLKSQGKILNPKQTLKENEIKENSQIIVEFVILQLCIVYKGQMENLEVSLDTPGEELKLQIKKQYMIENDFDIIINGVKMDTKFQLKDLNLQNKQCLEIQEKQKQTQIKIIEQTNLKNQQLQQQGYQQGIQNNQINQQIQSKQYQQSNENLQQQQQQQQQQQQQQQQQQQQNQQGKNQKIVIKEVPDIKLIRNNEKAQPNIKLISNNWKFTIQFQWETQIQEIIDYITNQYQIKDQLKLTFNKNTLQPNKTLEEIGVKDQDTIYVEQIQTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WFU4</accession>
    <name>A0A8S1WFU4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tubulin-tyrosine ligase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0900096</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8187891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WFU4"/>
    <dbReference type="OrthoDB" id="202825at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_125, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069">
        <property type="entry name" value="TUBULIN TYROSINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8187891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="427" mass="50989" checksum="AC5BEC59606D32F7" modified="2022-10-12" version="1">MLQQQIQDQNEINSSFNLIPRSSYKPLNPKVKVFYYYVAPGNNSKLIISAMQKRKNWQESENYLFHFQWNMNDKNFQYCSLNQSQIQIVNHLPNHRELTLKCNLIRNLKQFCQTNKKYVWDITPLSFIVEFNAQENNLNKVLKEFTLAYEQFQPLSSMKTQFNYFTKHNSQYFLPKMHHSFLDEENNYIWILKPNEFNRGRGIQFFRTIEELKSLLKNFNKGSSEYQFSQGKIKSSSFVIQKYIQRPLLLDGRKFDIRVWVLVTYTFDIFIFNQGYARLSSDMFDLNNLDAFIHLTNNAVQKYSKNYGFFELGNQMSYSELNYYTNGEFTKTVLPKIKSIIVLTWMAVQNTFKKCKYSFEIFGYDFMLDEQLKPWLIEINTNPCLEESSPLLKELIPKMINDAFKIVIDPLFTKINEPTDWEHLLQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WG84</accession>
    <name>A0A8S1WG84_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0890187</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000089">
        <property type="protein sequence ID" value="CAD8187657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WG84"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8187657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="100" mass="11941" checksum="916D572DBF185E91" modified="2022-10-12" version="1">MLQQQETKQKCQNIDENLLFIWNLIIISIKLKQIQLKINQNRFTANQRGTCQKNIAKLELQRSCCGLHYCFQITKGTQKNEYSQVKELTAQERLERFKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WH19</accession>
    <name>A0A8S1WH19_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0920022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000092">
        <property type="protein sequence ID" value="CAD8188653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="77931at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1486"/>
            <end position="1507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1533"/>
            <end position="1558"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1570"/>
            <end position="1587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1620"/>
            <end position="1647"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1729"/>
            <end position="1748"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1760"/>
            <end position="1786"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8188653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1877" mass="216864" checksum="5933DE4C0869359C" modified="2022-10-12" version="1">MDPFVFHDPFQKITQYCFDGICHHEFELEFYQVSCFFDRFPNTLSYFNINLEYCNHIGINTLTFNLYFDLEEDSCLIVPTMKSKASLKKDIFTLNNININVKTLNPFFLVFNNFIFFEDYDKVTFINFGFIFEEYLQTNIILLNIHNQVDLSLINCTIMNSSIINVTSLFYAEQFGSIYISNFSIINTTIKNSSLFNLQLLQLQGQMTIDSLFIVNCTFIDSQFVQFSKSLQNIQIVNLTLDSCVFQNASLFTFYLNYIKVTTFETKNILIKNNYFRLSYFIFCSQIIHIVLNNVILDINRIEKSILFGFSYSLSANYIQIKENLFIESQFLQTIEVTNDKITQCSIYELIVEKNVLQNTNLFHIFSNSYSNKLFISISSVQIKQNIRSINTDQSSQIFNINSKTLNLNNFFISDNQEIRIINLYDNEDIKLNNFIFENQIVHQKVSVSKNCLQISNLQNQLILIKGFDQIQIEKCKIIKQSSVDESLIEIVSSKSKQQLGTIVIKNIEFYGNLLQLFSQVQSFSLLAIISDKQINVVMENVKYQNNFMHIYSESALKQSATLLYITSALSYISINNLNCIQNSLTNSSNSFIRISSKQLIMSNILIKNHNILPFNIWKDYYPISIENENDQEEINLFIMQIYHIKVIGGAAQIETSKFQCLNCSFQEILVAKSSVFDIVTSNEGIILIQNFSVNSTTYSQIEQVESSGCLSLNSQNSLLTLSISSARFSNVINRMSTSLFSITPSLLQNYIIIENVNIINCISLMNQIIDIQFINQIVALNQIKFINIKIIQDQQAWVTYFQQIGIISSSEILEITGNNNALIYFQNCDIQIRDLIVEGILIAPVLKLINVQNLLIFNCYIENILSITSQDLIHISQTIQTKSFIYLKSINIREALLLTSNSFPYVRSSKLNADYFIGGCLIMNNSFLIEQPEDYLSSNIIQLQKSQQIQNSIIYIKSQSNLSNIFLDMIKLEYNNFQTSINGIIYFDVASFQKLRIQNLDCNNNFIKEYGCLHFVGNNSVNSRIQIKDSNFINNKGTKGVAIKVTDISLDLKNCKITSNFASTQGGGLYLRITSKLFRIIKTLIINNHAQEGGGIYFEKDHNLNTENFYYSYLFFNQALNYGNNLVENPTHLSLFINSKEMPAEIQQKDTTSISVLNIKPYKIIEQGKYIETQQLMIPSNQEINVYQLFILKRSLYISYIQNMSLQFKNSKGELLFNLISSTCHVSSVLINKDGGEVVGLTKNHILSYESLNNNFELGALAFSIDPYELNYSHLKIQVNCKTSYSQNEFTYILNAKSLKCQLGEFYIENGCQICSWSQGFYSVTYNAIKCSIYDKQKFQNITSNMINLYEGYWRPNYLSDYTESCYKNPQFCQGGWGVGDSTCILGHKGALCEMCDVYNQKGDGKFFKEQQNFSCQSCSKNDNIVLPFLFALFQSLLSITLSLKSINKSNQLFSSLKIFFRLSKLIFKLSQDHEGILVKLLLNYLWIFSVIFTFNINFSFSFIFIEQSSNSFYFMVNNLDCYLSDLQTQLIYIKIFVILILMLLQFNLILALSLVYHSITKNNMDNSLLSNTLLYLYVFNYGGLIKMVCSSLSGRQISNINYIQGDVSLLYDTRDHQLWIYSLILPLLVVYGCIIPFSLFLLTYFKRDILDKIKFRRHICYLFNEYTDNNYYWEQIKLLQKAIIILITTYFENNILLKTSLLGLCLLSYQAIAVKNKPYIISKFNNLDLASGQICSIAIFLAAIKYESQNLNNQFFSVFLQILILIILIRLCYPFIFNIVTVYYQKYSFLILTKIHQSFKSLNLNKQCITILGNYLIKENERKQKLKRNFQKLKEYLISKSRSKIQSSNYRAANIKIHLRSTSNSGQYLVEALNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-02-22" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WLJ1</accession>
    <name>A0A8S1WLJ1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0940043</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8189471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="64" mass="7767" checksum="C3A7CCBA09234E49" modified="2022-10-12" version="1">MTNCVXFISYRPSVVSQDDFDVICKRNQNNYKRKKLNLLQKEKKQLDLYANIYNEKELKNKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WMT3</accession>
    <name>A0A8S1WMT3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Histone-binding protein RBBP4-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0940072</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WMT3"/>
    <dbReference type="OrthoDB" id="427795at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022052">
        <property type="entry name" value="Histone-bd_RBBP4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050459">
        <property type="entry name" value="WD_repeat_RBAP46/RBAP48/MSI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22850">
        <property type="entry name" value="WD40 REPEAT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12265">
        <property type="entry name" value="CAF1C_H4-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Histone-binding protein RBBP4-like N-terminal" evidence="5">
        <location>
            <begin position="15"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="286"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8189529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="396" mass="46276" checksum="F99F0A2A3D4F368C" modified="2022-10-12" version="1">MSGENEIQEERNNEEQYRNWKQNIPFMYEICINHQNNWPSLTVCWLNELEIDQNDNELHRLVVATQTNNQEQDYIKLLKVATPKQINEQLDYTLLNNIWKTQAVGKVQEELQIPVEVEINRIRQSPNDQYILAAQAGDGEVGIYDLSRQSKVQALKGQMKEGYGLSWNLYNYGQLLSASYDHKIYHWDSFTGQLIKQYNFHSAEVEDICWHPQDPNIFISCSDDKTFAICDIRTNQGVTIQQEAHSQEINCAQFNHFQSNLFATGSNDAQIKMFDINKPEEDIHTFSNHEDAIYSLQWSPHQRNLLASGSVDTKIVVWDYYKIGNEIKAEDEKDGPSELLFYHGGHRSKVNDLSWNINHKNLFASVEQEKNILQIWKIQPQLWEEDENDEYIKSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WPC0</accession>
    <name>A0A8S1WPC0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0960142</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000096">
        <property type="protein sequence ID" value="CAD8190481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WPC0"/>
    <dbReference type="OrthoDB" id="292517at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="298"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8190481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="353" mass="41481" checksum="36ABC6EAB7722E63" modified="2022-10-12" version="1">MDNIVKQVDDEFNEGKQDSFQQPERLSDNLARTVFFDSNNLPQVECYYHKGYYISNMCRAPQCIIPLCPLCIHLHSKEHVNEGTYPVFESLEVLLNQASDHVNNELKKFTNSYYDIKKHVNTFEVHADKTIKKIREIKKRIQDVVEQFFNGLENEVQHKQKKNVNNQERDAKHLLSMIEERWRSMKQMLNTFQSSDCLTALIPYFTTTFQEDNHVYYKKIAEYINAFPTVSSEVQIDQHRASELSVFLGSIIRVQHTSIPDFIGIHQLPTTNVTESTKINSLQGSRIKEPLRVTIQEKKTQFESQDHRKQPVQDVRAQRSPPPNSQSMYPYPVHPSMHQYPPQHYLPPQYHRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WQ39</accession>
    <name>A0A8S1WQ39_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0960043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000096">
        <property type="protein sequence ID" value="CAD8190289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="302428at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1064"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1231"/>
            <end position="1253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1335"/>
            <end position="1356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1540"/>
            <end position="1565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8190289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1605" mass="190708" checksum="2AD7A5B9F554DD1B" modified="2022-10-12" version="1">MKKAIQKAKNIHYILHFDQANISESVLLFTNFLYLAQLLNLGKHFEPNSEIVTIYSLLKSTFILNLIPEKYFPHIQILLFIYNLSLAISILTVVLLNINHNSKNWILLMITINVTLNVYPNVFFIPNLWLNIKQCFTQNILYFFISLINIIFTLIITFFIVFFQRGDSLAQNENITNTIIIIRILLKLTEFITIYLSFLDDTISFILEQFILICIIFLNLFKLTAISANKVQLSILFFLFNLSLLADYKMMDYLLISILILSLQQQIQFRKLQQILFNSDTILSCQLAERVYKKCLIDKQARIQLQVFKNNHKCTKCKAFYDIIECILKKTCKNERDKIIYANFISKKWPLRALVELLKEQNEDFYFQSAILTFQKTNESTFDITNYIYTSYKSQIECESIKSKLLSLITFMIKFWNQTIMNQLGIKQFYKSVQQVGKKIDQINKIMDKVYDIRNCKLKNNQFSDIITLRLLQIYYCIANVDLIKAQQMEDKINDLFRSDKFRQSNTIDNNQLATNRSMLLTTSLIYNTNKLLQPNYQQISLFFDAQIEEVNFIKTSMEMMPQFISNVHDQLIENFIQKGQSRLCQQGQQTFYQNLQGYIVPCNIHLIPLQGQNDYLINAILTKDLNYNQCIIFGMNGRLYGMTQDFFEFSLQSMQFDTYTKNLSINDLIDKGSLIQYYIENIQEQIQLLKQSIEKHQNYVILEVQSQWQYPENHLNCVFNTNQLIKQQYNSSMNQMLSFSNFMSQKTYLQTSKQTEKSAQIYEFDESGSQNKELMIDGVEWSILNQSYHNSIRQMIDQFSEKQKTNRIRLVMIYSLSFKKISFGKRSLGYFVMELKDYRQEFTQKTTSQYLTTYQTKKTESSQKNKSSYSFPASDFDDIYSEKPIIDDDLDNQIRQINLKNHLLYLNKLEIDKRTIIQNENQISSINVTKQKMQSYDFDNSSRLLKIQTDRQPKKHLLTTRIELQSINIQQDDDDIMQEVEKEFDEIAFERKMINNQEQDDDNFDIQRDRNQKDCYQKQKTKIINEQLSNQTFTKQLFALKDTFQYLEKISNNKFTINSLQKFIYFSFCVIILLLINVVMDSLKAYTHVQYNDNFIIQTLDEIKFHRICAIKLSLLITKLLGEYSIINKNQFIFQLQQKQIQNIYDYNTYDQQYLLINTYESNQQVTSEQFNILLQQFDHDMRKVYYNAEQITFINSTFSFTNDTQFIFNYLYDIVIHKLDNYDDQQVDYISASIFIILYILLIVYQIKFLYAKQKVIIKLLKLAHQTNINKIQNQIARLSTIKDTFECNNSKNWKLTSYVQIISEDVQHEKSQKKQNHDLEGNIGHQYLYPNIIAILFLCLFAAMGLFFQLIYYQKEYLNIQNQFLKLNIMIDHSLIYGSLIKTYYILKITKSLDNSIIENFNESINNQIDITNNIIDDLERLQESNLLDSLINDQCLYFQDMIEYCKNKQYPYNEMYQIIERGIISLTNNIQKVKNTEFNYELTTKQFQKESTELIEYINSQSFINTFLIYFLESVNILDKEIEKIYQVGQDQFNNYVLMIQLYEIGVGISIGLMYLLYGYLTQIYHRSDFKIIILFLRTIPNEQMQLKNILHQIKNIVQEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WTE5</accession>
    <name>A0A8S1WTE5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1000154</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000100">
        <property type="protein sequence ID" value="CAD8192057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WTE5"/>
    <dbReference type="OrthoDB" id="423343at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF179">
        <property type="entry name" value="OS10G0565000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8192057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="350" mass="40327" checksum="5F6D5CDCA5B5EA2E" modified="2022-10-12" version="1">MLEQSGHTKAHSEGFQTVFESDIESLQKRPKSSSQENYKKLFEIVKDCSHSKLQQAHSDEIPEFDSLEDSCQIRPSVCTNKSSKQSQEREEKLFEKELLSPQNEDDYKLIAMSEREQCKLPSIIMKNGGCYTGYWYKKKPQGQGEYKFADQSRYNGEWSNGYASGKGKFFDTEGGYYCGDFHLNYMHGKGVYNYSDGSIYDGQWFNDKYNGYGIEVKSDSHYKGQFRNGLKHGHGILVFSNKEKYEGSFINGQFEGKGTFMWPDGRRYQGDWKNGMMHGQGILQWSDGRVYVGQYVNDKRQGFGTFQFADGRKYSGQWMNGLQHGLGEFTEQHGQITKGIWREGKLFSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WXN5</accession>
    <name>A0A8S1WXN5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Histone deacetylase interacting domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1060070</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WXN5"/>
    <dbReference type="OrthoDB" id="288083at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013194">
        <property type="entry name" value="HDAC_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039774">
        <property type="entry name" value="Sin3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031693">
        <property type="entry name" value="Sin3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346:SF0">
        <property type="entry name" value="SIN3A, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346">
        <property type="entry name" value="SIN3B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08295">
        <property type="entry name" value="Sin3_corepress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16879">
        <property type="entry name" value="Sin3a_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00761">
        <property type="entry name" value="HDAC_interact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0678">Repressor</keyword>
    <feature type="domain" description="Histone deacetylase interacting" evidence="3">
        <location>
            <begin position="268"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="391"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8194192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="874" mass="103419" checksum="F8E69667252F173E" modified="2022-10-12" version="1">MSDDILSKSNMGEFGEDIDQQLYERCEQFCQKLRRTNQQLYKQFSNIVSQTSRIAKESLGQMLSEEPELLKEVNNLLEDDVKFKTQKEKLTEFVNNESNQSKAMETILKQIQNGQLDGHQNKDHILDLFSNDPQLQQQFLQSFKKKHDQNPPKKKIEENRNYFQRLLDKSGIAVIDMNELLFWDKFRLRVIQQFPNDYQQFMNEFLKIIHLYSECIITQMEVMELINQQSWLEREYIDDIRSMLTTRVIARRIQTPLFKPLKDTDFAQVDRVTRSYVRMPIGYAKANNNPQILNHSWVSVPFGSEDQSFLIMRKNTFEEQLFKSEDERFEFDVNIQQIKRTINLLQEIIDGNKDEQVLVNKVIDMRILQQLYRNQTQDQNEIIQLFQTKPIESAKILIKRVKQKLNELVSARNNKAKQVWETVSTINFHRSLDHRSFYFKKNDKLVINGQRFVREIEEYAKNVLIKFLGTKDHVFLKRKADNIQFTFDDSFLNSLAQMTNIKPFLPKQMPLAPPIKENVRFCPLISLWMGNEQILQECGQFIIHYLQIMGGNNQLDKRNGTFLMIKLINHFFQVPMKPMQISELTLNDTKLKTEIQDLEIYDIHYNPEETTVSIPEIENEPPGHFLNLQPRVKQTTYVTQNLYILLRFLHTIFQRLEMAYIISKQNNLGKDKRYNLFKSALFLSLKAKDFKYEDHLRSLFGKHGFIFSTLEKVLHDAAKQLAICASDPVTTSYFEKPAQFSGDIDKYFQVVSGIVNGEGIKVSDLELEVYYQFQILKEEEMLLRLHETLQHCKPEKKPIYRIACWHKACFISMLPGYGGHLQEKCTSWKQPFLMRNMMRGERRMMILNSIEWMVDELCHFKPIINNGEDYLKCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X347</accession>
    <name>A0A8S1X347_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1080069</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000108">
        <property type="protein sequence ID" value="CAD8194989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X347"/>
    <dbReference type="OrthoDB" id="10291464at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8194989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="308" mass="37250" checksum="27C0CA2DF8AE3E24" modified="2022-10-12" version="1">MRSQYILSRTDKQWENILKQIIQDEQFILINFDEEKLKFSKILSNFFKEEERNDQIVDFLRNSYINQETFEIYLFNQIKLFLTNYHEVFNKIILLKYEKYHFSVTYSIFQGSKPTILIQLVKSQYNKEIQSIIINTLINLKLLTNLIRIINGNQNYSKDKFHIISTKLIYQYFQAKLHSKTFSFKHCNIKNIIQQLKYYFYPNSFVRLLENTVHIKTIPCILKLLLLIIIESSNSNVIFIKTLNIRGQDNCLKIHSKFNIERFQVRYLQFEYFLQAIVKCISSNEKQIEFELYQESFLPYCKPMFLKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X703</accession>
    <name>A0A8S1X703_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1140078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000114">
        <property type="protein sequence ID" value="CAD8197031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X703"/>
    <dbReference type="OrthoDB" id="307968at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="7"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="214"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="477"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="541"/>
            <end position="736"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8197031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="740" mass="88959" checksum="F58C487202B5DAEB" modified="2022-10-12" version="1">MEVDRSKEILSNLLTKQIDLNEQLQNENDELNINLKRLLNLLNQKDNQLEEIKDRIIQVDQTENQYQNTISHQTQQIKQIQSENQELLLQIQQQSEIRYKMEQELSKIIESFERMQKINQQQQEEINLLQERNQQLEETQITNTIQIEQYQKQIKLLVSSNEDYEKQTDILLKQTNELKQIQIKLLYNLDAIQKEKDEMTTQFDEKLIQFKSCFQSTAENTKDLTNQVNDLNNQNQILQKQNQQLIDQIEDYQSLSLKLEQERDKYHKDLEITIQNNSRLQNNNSNLENQLIQLSQDQESSIKKMKYMIEQIDELEQANDSQLKQNNELKQQIKKLQFQQDQVLKEKEVIYEQFEQIQKNAQNDHEGNRDKYIKLDRSYKELHCQYDELLNKYKIQNQTFEEQQVQIQLNIKEINELKHNLRILQESERKHSYNYEVISKEKFMITQKLEESYQIIKDLRDQCQIQTEKLQKTEMFNKQLLMENEQQQKQIKAQENKIEEHEVHIELLQVQINHKQTQQIEMDRQQALKQSTTKKSQSVNKENNNNELIRLKKQNTELTKEAISLNEQILELKNVINKLNDQLAQQSKVKTGLESRIQSLIKVVEEFQQKQIQKQQDSITKEQFQNQVEQIKKNCYIQIQQLTRENEELKKLLQTQANQTFNSTEEVSSLQDTVADLNREKVNLIRELNQEHEAKLKSEVERDKLKQQIEQYQLEIENCQNQLDLYVKVLKQMDEKLKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X7F5</accession>
    <name>A0A8S1X7F5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Insulin-degrading enzyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T1130154</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the peptidase M16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000113">
        <property type="protein sequence ID" value="CAD8196809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X7F5"/>
    <dbReference type="OrthoDB" id="952271at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000079">
        <property type="entry name" value="Coenzyme PQQ synthesis protein F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000012">
        <property type="entry name" value="Protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000108">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000116">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050626">
        <property type="entry name" value="Peptidase_M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032632">
        <property type="entry name" value="Peptidase_M16_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054734">
        <property type="entry name" value="PqqF-like_C_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690:SF18">
        <property type="entry name" value="INSULIN-DEGRADING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690">
        <property type="entry name" value="NARDILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16187">
        <property type="entry name" value="Peptidase_M16_M"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22456">
        <property type="entry name" value="PqqF-like_C_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="9">
        <location>
            <begin position="100"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="10">
        <location>
            <begin position="259"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 middle/third" evidence="11">
        <location>
            <begin position="448"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 middle/third" evidence="11">
        <location>
            <begin position="625"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="domain" description="Coenzyme PQQ synthesis protein F-like C-terminal lobe" evidence="12">
        <location>
            <begin position="905"/>
            <end position="1003"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8196809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1100" mass="127221" checksum="229415C57502BCC9" modified="2022-10-12" version="1">MQKLRREAIERAKLNSAFLVTLLTLFICDLSGLVQLDKPLQYVSAANAIKDQIPERNFISASQTGTLEPLQGQHIDKVNVIKPIIDHREYRYLELENHLKVLLIHDSESEMASAAMDVKAGSWHEPNEYPGLAHFCEHMLFIGSQKYPQTGFFDDLMAKGGGSSNAYTEAQNTNYYFEITVNHLGKALDAFAHFFIDPLFNEDAVNKERNAVNSEYEIDVSTEDWKVVNLFALLADPKHPASRFSIGNNDVLAKEGVVEALKKFYQDNYSSNIMSLAVSSRLSLNEMEKMVKVFSKIENKNLIPQSISGFPYQFGLLGKYKTEKKLVLLNWQLSGREQFVHQKPLELIDYLLNNGNLKDYLKEKQLVIEFESSIFIEQNHFVNYIIELTLPEQPLEDGNIALEITRIINNYIQQLEEWLKDEKYLEEIFKEQSQISKINFEYMEGPQEISNIAKNLNKYESAEVLSSSFIMDVFDKDLILKYISELKKTDNLLILIGDDEYQYTDSNLKGNNKEFLKDKKLDKKSEIYRLEYATQKLTKDSIKIITQKDSKLQKIFTKPQENLFIPDDLKLISLCESNQSKLPIMVHSDQLKPLDKEGKLNILLHAGQDLQEFSEDQCKKEENVYQKNNHFPILLNKENNQWWKVSTQFKLPSVYGAISIQYTKPITIRQFTSARIYNFISDEEINKQLRQPLTTGYSVELDIGKQIEIKAYGFSEKVRSFFNSICGCINPFKDKSKSFIELEEKKEESPDFIRAKQSLITSIKDIFQLKLYEQSFQLYLPSILRKDFFNPELVLQQIPDITEEQMLSDIKDILQNSIQSSLLIGNIDQKNAQDLSADLQNCIQDKEAQIEKQTRLPISVLSLNGKNWVFAKFVEQDKGDLNGVTLNYYQIGQRNQQNYALMKILQPVLNSQAYNHLRTDLQLGYVVAMKFKQVSCIDGALFLVQGNKELPMKVNELIEEFLVQFDEYLKTMNKKQFEHLRHSAIVELRQKPQTLSEEADRLWGYISSDDYSFEERQVTIENLKSISKEQIIEFYENVFINNRSKISLQLYGEGMVSQTLSLKNNEEFDSYIKANIPEGASLFTDNESQYYDCPNDVSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X7Q5</accession>
    <name>A0A8S1X7Q5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T1140072</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000114">
        <property type="protein sequence ID" value="CAD8197019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X7Q5"/>
    <dbReference type="OrthoDB" id="346907at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000698">
        <property type="entry name" value="Serine/threonine-protein kinase tousled-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974">
        <property type="entry name" value="MIXED LINEAGE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974:SF23">
        <property type="entry name" value="TOUSLED-LIKE KINASE, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="348"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="79"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="137"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="231"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="295"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="377"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8197019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="625" mass="74343" checksum="E4CC60616353C3E2" modified="2022-10-12" version="1">MSEEIQKSNSDQQLITHYFNQNPENSQDRQKISRKRRIDTELPKKKIVQKLKSDDESSNDNNQQALVNPPNCQTIYKYLKLTKDVNNEAGNQIKALQQKNTEEGKQKQQGNDKTVYIKPKESKVILQSVQKQSDDDANKLQQKLMEKEQIEKQLRQEKQQLENERQELILQHNVFKKRTQEVFAEALKQVEELKREKMREYLERERYRLGEFVSSRNNVRFVEEWQDGYEIKQVKQNLQKLENERNELEKQKNEIKDKSLSKIQKDKQTKLLFELDQQNGDLDNNQRKLRIQFQLSILKKEEEELKLKLQKLEEEKQQLAYKTRRFMEEQSCRYYRAEPKWPLIASRYQTLGLLGKGGFSEVYKSFDLQEFRVCACKIHYLNPQWNENAKNNYIKHALRENDIHKRLKHINIVSLYDTQEIDQDSFCTVLEFCDGTDLNQHLKKYKLLAEKEAKLIIRQVLAALHYMSCSPTKIIHYDLKPQNILFHKGEVKITDFGLCKVLDYDTTRQELTSEGLGTYWYLPPECFLEQPNIQISTKVDVWSVGVILFEMVYGKKPFGDGMSQERIAQERVILNSYQVKFPQKPNISQECKDFITKCLTYNMEARWNISEAYYCNYIQNLKTTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X7X7</accession>
    <name>A0A8S1X7X7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1140185</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000114">
        <property type="protein sequence ID" value="CAD8197229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X7X7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8197229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="70" mass="7981" checksum="D54E92704EDAE948" modified="2022-10-12" version="1">MSLKVKRVDLFQLVLIILNSLFQYFLKQLSSLPIPQVANGVLLPPWRQQRGVEVKEDSEGEGGEKIQKQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XBB0</accession>
    <name>A0A8S1XBB0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1190044</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000119">
        <property type="protein sequence ID" value="CAD8198526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XBB0"/>
    <dbReference type="OrthoDB" id="302595at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8198526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="477" mass="55589" checksum="BE008DBFE91B341A" modified="2022-10-12" version="1">MSSKVKNVYLILRLRLEELRVVTIKTFQIGINILLFASLRGDQKAKNQNSLQQYGLTSQKQLQQAQQISSSLYNHNFGSFYPEFIRASITLYLVGLVINLLHTEIMNVINKKFMLQKCIGTIVIVFLHILDLMIINDYLILNSEYSNLTQAVSDIYVDSVRKQKDMIQLFEAVIRQLVNINRFLSIFFQASALWGDGIRKLIKNKRKTWGTSDSQLSSPQPQEKTQLNHIPPVNTVNNNKMKGQKKEIVQHSGKKKKTIQQQQQIEKSTEERGQSIEKRKKKILDKKPSKLSPKQYEQQIETKIEESQQLALSKQVSQIQKDEQNNSTQAGSNGNKSLFDHKIKSNRSESLPSKLCSQIEISQKYLRYSSQSQKKHIEQKKQIRPIYDKLYKFNLQTNVDKYEIKVHSIIEMNNSINKIAQENSWNSFLTINVKLNYWKEAYQQMQTQEIKEELQKVIAQYALYLMNQHAQNQEIVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XFJ4</accession>
    <name>A0A8S1XFJ4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1230046</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000123">
        <property type="protein sequence ID" value="CAD8199980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XFJ4"/>
    <dbReference type="OrthoDB" id="297138at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="256"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8199980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="294" mass="33453" checksum="DA04A22DE906949A" modified="2022-10-12" version="1">MCMPIGILRCVVSLEAWIVMICGIAAFIFTMIMQIHYSLMLQANEGESWMLFGQGMLTAFWLFSSYIIINGVCGIVGGQHKKPCLLLAFNVGNIIIIIAFIILMVIGYVLADETRKLTDKDIQQNENTCLDNRSELNTLDWESKDLLCSIECPCYYTQLNGPLAKNKTLWGDDKHPERPTKVQDCELFQKTQNTTVKYFSNYLGEIQKNTGCTGWCVPYQMQIFYDINIVPKDPQLYCQYVVIQKLTEMGMLMGDIAAGVTAVMLILITLTCCLCFHPANKDQDFYKKMAHYEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XH92</accession>
    <name>A0A8S1XH92_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1250102</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000125">
        <property type="protein sequence ID" value="CAD8200730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XH92"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="236"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="297"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="250"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="283"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8200730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="577" mass="67512" checksum="06B63AED45A9C3E0" modified="2022-10-12" version="1">MNSSSSSQLLQKLQNLYSSNKKQSEHSVSPLQPRPFVCRELNQLIEKSSPVPYLQVKQHDFEQWTERLIKPTQKASCSSFTQNENVFDIHLQKYSQISTISQLTFPVLGQIEKKVKLLKYAKENDHNTCAAIIAKMKLFCLDNAQTCYDFYRSTDLILLKYMIFLLIQELNQRPTIEKQEISNDQTSQFQSMIKDIISIVYEIIEEVKSKSFSGSQIDTLNKQLAKIQNKFKLDSNSTHKSINKSHHQRISSCNFETSRTPNSQNHSNKESNISSDKFKSAKKQQQQNNGSGSQKIIKFQIEELEQKKQLISKLNEQISKLQNTNQQKQAQIQDLNDQIISIKQTLKQKQEEIEQLSQSLEALNQNNIIHQEEKEQYNNLFHEYEDENAQLKSNLEQFQNTIELCKQQFENQKQETFAYYQNQCNQIQQIKENEINHLRNELNENQVIINHLLSDALYFGNQYKNLVDKIDNLKPESIFNDLNQIQKELFYTENILNVKLNAISNFSDNLLFDAGQEYLSQSLSQHKMMNSQELTNIQQQNNSKKNNSNNYIIAQKNQFEIMEMLLIQSQVLEKFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XHL5</accession>
    <name>A0A8S1XHL5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1240171</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000124">
        <property type="protein sequence ID" value="CAD8200497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XHL5"/>
    <dbReference type="OrthoDB" id="308006at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="425"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="453"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="586"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="613"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="636"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="676"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="699"/>
            <end position="725"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8200497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="920" mass="109253" checksum="4EA2A90004535BA0" modified="2022-10-12" version="1">MSIEKDCLIIFDEKEEIPFQIEIEDQKNKQYLTYPLSLDKFDIIRFISKTQKYQLIKKIEYMKKYIEIVEQPLQFTQKINQNLENYLPDVMFGPKSLQPNAGQYGFLKEYQTQVLQQSQAQISFEQNTTNIPGSNVAILIYKGETYKSLTNQLQKYSNEQTKVKPIILINGKSAFKKLMRSNEALFLDFYQTKYVPFQQKFMTSNHHSIYFQIENEIGYFFVFGRQLEQLDLIKWIYQGILKLIQPKFVVIASSNDVFLENINILQLFTILENDKYFGISCLKKIKYNGRFWNSQQGDFADIALKLDSMFKIKQFHNPLSCIYEWSRIEPIINDYIRKLESEYFTNSWALGYNSILPRIMIEYSGKELKIITNYIIEQQFSENQFEIVFNQFCEYQQNINNQLRKCRFHLFKNVFQWIISKIQVLYNYFGISICFFFSFQCPYQLIYNLFEESIGYTALAVILPIFYTINVLLFLLLTQLYHFNDKIIEKGYDQEIVMMAQKNQKQEFLFQTSVSSLNIDVLFTKEYDKQNEINFEVQEDEYVKNTSYSMDNELLQKDELYFLYKFPQLKYISVYINYLIDTQNYLDFGVTAFIFANLIIIHGKILLTEQSNYEIILLIFVLFVLIFQLTTKKLGIGWIFVKFSMITYFWQFLQVSKQVTSAPQRSIEKRKLGTQLMLNFILFYTFIAIESYYNYSGYILIGVFGYLALVYIIIGVFAFIQYICYNSSIPKEKKIWEGQNFVENYTLNHKLHQDANHILVENTRKLVADTTQGKKAQQLMLQTQLMNDQGYINLTNTIRIQIKEELEKHYNVTQFDLNQIKKLAETAIIQQIQAQYKDQKNQNLIKKAEDTTQTQQGIQQNQNEKQQQQNSKLNQDLLNSCKQSDIKKIDSNIQKLSEIKQEDMLESNRNKFNQFGQSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XJ87</accession>
    <name>A0A8S1XJ87_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1260005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000126">
        <property type="protein sequence ID" value="CAD8200818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XJ87"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8200818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="85" mass="9891" checksum="3B58AD38D5BDCA62" modified="2022-10-12" version="1">MGIGQNIQFISLENTIPVEKIFQLIEQNYHIGYYYQNFSQDSNILSVLTANLIYFQLEICSNKILNKQILISLIQNSVHQISKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XLC6</accession>
    <name>A0A8S1XLC6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1290108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000129">
        <property type="protein sequence ID" value="CAD8201943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XLC6"/>
    <dbReference type="OrthoDB" id="305068at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="273"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8201943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="673" mass="79740" checksum="AC68F3B4170C8792" modified="2022-10-12" version="1">MRKSVQLEMSKPLKYPSNDVSVCWPSFQSCNFSSMPICNVAENNPHMSLSKFSDSESENNHAPKKLEKLCSYHTYSNEESRKSLLVQSEKFWYSKIISSNIINITLFILTFYLPYFYYENFIESDPLYFPVLLFLLILELYTQFIKLLGQFLKNKRFSVSLLNIAILLTYTLCLKANNIILFTILYFLLFQRNLKFIQKMLSLILYINYQQIIITKLVILILFQIHYFACIWGVELKKTNQEDLIYTECFYKSFLMFFLKIDQLENQNHYLIITHLITSIIIIIFVFTIFQQCLLQMQKCNLALKTYRLFLKSNMLELKVKLILFHYSGLFLLIENQMSIKNDIKKKYLLEYIKMKLIRQELNHLNFLSQSALNQISVKGVLGQNLKQQNQQAEINGLYIIIAGNIDIEFMGMKLQIDNRKIINLCLIEMMNTQQQGRVKFEQVDMNDLLYFYINQNQFNDCLQKQQEKEKYQMIRDDVIFNNNTQELNIQCHFCNLFHPTFNCICFNVKRRFTYEKIYQERSPMFQRKNNNRIKAGIYQQITHQGTNNNTVFDISSDSFEAFSDYSSDKVSVSNLPIHFEQIGKQTSITQIHKDILQIDMVSDKVTDPIISSTLIKHLQVPEQQHKNHQGISQTGFQDFDSLLDIDKICEYQYYKANFNLNNVLMKLNQQKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XPX8</accession>
    <name>A0A8S1XPX8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">T-complex protein 1 subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1330066</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000133">
        <property type="protein sequence ID" value="CAD8203175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000069">
        <property type="entry name" value="T-complex protein 1 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8203175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="313" mass="34693" checksum="5BC05DF1BD54F0AA" modified="2022-10-12" version="1">MQGAPVIVVNANTKREQGRKAQLGNIQAAKAVSDIVLTTLGPRSMLKMLLDPMGGIVMTNDGNAILREIDVQHPAAKSMIELARAQDEEVGDGTTSVIILAGEMMVAARPFIEKNIHPTEIVNGYFKALEDSLSILDEIAQQIDTEKKEEVMKALQSCIGTKFAFRWGTLISDLSLQATRIVLRGGNVNKLNLEIKRYAKVEKIPGGTLEESCVLEGVMINKDVTHPRMRREIKNPRIILLDCTLEYKKGESMTNMEMTKESDMTDALQQEINEVALMCNDILKHKPDIVITEKGVSDLAQHFLLKGXINQMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XUY5</accession>
    <name>A0A8S1XUY5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNase III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1400106</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000140">
        <property type="protein sequence ID" value="CAD8205346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XUY5"/>
    <dbReference type="OrthoDB" id="286154at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14950">
        <property type="entry name" value="DICER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14950:SF37">
        <property type="entry name" value="ENDORIBONUCLEASE DICER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00636">
        <property type="entry name" value="Ribonuclease_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase III" evidence="2">
        <location>
            <begin position="336"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8205346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="725" mass="85042" checksum="69737F02A6901C15" modified="2022-10-12" version="1">MADIPSVQFFGQMPPQISSHYTTFEDNQNVLIYLNYFEIDNQMLILLSPSPIEYSQQSFIVFSSTIRPRYRVSQKLMISIDQFKSIVSFNILLFGDLYLTNKTQSQPTNARNFQTFITSNPLPQINILVVPGVYSINFDQIYEFLESKLALKNECIQPQPKYDILVYTTNPYRVCNIQFQFDKTTQLIDYFYYLIDNHIYSDINEIQQIFLLDNIKGTLLDFLSGKNQQPSAYFIQLIRKNFSYNTQDNRFYILQQIKGFNQIHQNADFVKIFCQNCEDGNKCTLLSSNNQQIFFSGQSSLFKYPLSIKFMDLVSRTFSNLICQLLFIANAQNATYQIDRLANIDRIEEAISCPSFNPQRNYQNLEMLGDVVIKYLTSAMLFEDNTYKTENSLSSARVRLITNKHLSGIYGKLKLNTMNFKINCKKFLNHMMMIINEQEDPKLSQKQQADIYEALCGACYIKNYEFKDLMDFFKLTKFEFKGKVQQFYKGNSLIDFSNDSQDTDNDNDYPLKKQIQKRPFTEILKFNQPFKEFEDYLGCQLNNIAEAMTVDQFERLEFFGDAILELLVIVNVHKECEKVYYNSRQQQLCREGKITQKLLLCPGMLHTAKISLLDRGFMGTMAMYHNFHQYARNLSRETQNDLKKALTLLREDQFNDFRKINQYSLQIPKIMSDLWESVAACILVEHGWEMVVKIYGELYKPYIKYFVDNISLIYDFYQSSGSLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XZ89</accession>
    <name>A0A8S1XZ89_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1460037</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000146">
        <property type="protein sequence ID" value="CAD8207071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XZ89"/>
    <dbReference type="OrthoDB" id="10365515at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8207071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="601" mass="69661" checksum="0EF9AAA0A3B72A22" modified="2022-10-12" version="1">MTSRIDSGLLFIAANDIDNNNTSVILIYKPRQSLRDSLIKVMIPKRQKLYIFESQMAAGGFNHQIFYLNDGDEHNLEWIDKQNGLTNIIIIKQLQIMRQINGISNLKQNPIIELSQAIVLYQTKSQIKFIQTNLNITNQKWIKDEIILNMVGTIFDYLLQCQQCGQGKNVNIIQPLTLFKQEYGDDLNVVDQIECGGQYSLRILLVSKSNLQAIRIYNSTNSFFKSIVISNQTNYKCEKVLKYLNHMIVACRNSQNYQMVTIWCDNETSCGNVIKYSDLTTNVKNIYSMNFDYKYLIVVDAHPKNLKSMDTYLRLFLVTFDDIKHTVTYSQTQSINTVQQYGEYLVKALLHHRPNYSVNTYILGLMSNGALKIYNSSLALKVSTINIYTEIINNGGEIIQKEFNDFFFTEIPTYSVNISTIHLLFTSQSLNYKFNLVYDTDNYKLKSIAYNYALSRYYDAQTLPGMWIDQYKNIASIPYRVNDKKVFLFFQLPDNSVNDKRIVYSYGGISEYHDFTYTDQVSCTIQKNVVILGVNSKYESNMFYIKYYDLQLKYTMIIDNSGGNLQNQVGSLELSNTICSEQLQFNIRIKESDQRKENSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y216</accession>
    <name>A0A8S1Y216_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1430163</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y216"/>
    <dbReference type="OrthoDB" id="305253at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802:SF155">
        <property type="entry name" value="DEUBIQUITINASE MYSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802">
        <property type="entry name" value="SWI/SNF COMPLEX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="121"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8206362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="200" mass="24072" checksum="0788EC4D7DA610DD" modified="2022-10-12" version="1">MFPFAYIPQDLQSSFNFCQPLYQFPWFYPYFQYGNFSTNYLSQFPRINCHFQQPEIHSQQNIIKMNDIQAKFPETFQRSFQIEPIEAPTSLDHNQSIISKMPITILNKRKKKKFNKSKFFKKGHWTNKEHRLYLQFIESHIEIMSDSDQKKMNKIFKQMSDFIKTRSASQCRSHHQKFNPKEFVVLTTVKNSSQFKIANE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y4L2</accession>
    <name>A0A8S1Y4L2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">2Fe-2S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T1510068</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the adrenodoxin/putidaredoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000151">
        <property type="protein sequence ID" value="CAD8208570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y4L2"/>
    <dbReference type="OrthoDB" id="268593at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140647">
        <property type="term" value="P:P450-containing electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001055">
        <property type="entry name" value="Adrenodoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018298">
        <property type="entry name" value="Adrenodoxin_Fe-S_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426:SF72">
        <property type="entry name" value="2FE-2S FERREDOXIN-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426">
        <property type="entry name" value="FERREDOXIN/ADRENODOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00814">
        <property type="entry name" value="ADX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="9">
        <location>
            <begin position="14"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8208570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="131" mass="14840" checksum="9120A4A50AFA5928" modified="2022-10-12" version="1">MIRLFSKYSASSQKMVTFFFKNKDNSLTKVTAPIGQNLLQIAHKNEVDLEGACEQSLACSTCHVILPKQLYDKLTQPIPEEEDLLDLAYGLTETSRLGCQVQVDEKFENVIIQLPKATRNFYVDGHKPKPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y6Q2</accession>
    <name>A0A8S1Y6Q2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1520122</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000152">
        <property type="protein sequence ID" value="CAD8208963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y6Q2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8208963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="76" mass="9148" checksum="234E8B43A0413D3E" modified="2022-10-12" version="1">MLLNKILFKQIFKLPISINKLLINTRKQLQLKISEFYFKALLRLLLSNAYDFNFACAIQMAYYPVERIIFYQQIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y7D8</accession>
    <name>A0A8S1Y7D8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1550010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000155">
        <property type="protein sequence ID" value="CAD8209500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y7D8"/>
    <dbReference type="OrthoDB" id="10652662at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8209500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="180" mass="22145" checksum="C8FE06021084C501" modified="2022-10-12" version="1">MLRYSTTHHLFLVQLKQHKLILDTNQQLRKLKFKIQNVQCTKCQLGLVRNVEIQQQDEQSIKLYQIIINNSLSGRTCCLLIAQIKMIQLFLILNQIIECYNYHNYENLLNSMKYQIIIKKVNFSITLQIKQYILYIQINHNQHKQYNKRLHFQKQFCRYWRLNLLFIRERIIIYFLFKNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y7L8</accession>
    <name>A0A8S1Y7L8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1570082</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000157">
        <property type="protein sequence ID" value="CAD8210045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y7L8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8210045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="51" mass="6235" checksum="900647E84DD8F986" modified="2022-10-12" version="1">MKLRTRNLLRTHNQFVSLNIHYSVLFTDTFETNIFKIKRPQLRTNIINFQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8M3</accession>
    <name>A0A8S1Y8M3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1490015</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000149">
        <property type="protein sequence ID" value="CAD8207884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y8M3"/>
    <dbReference type="OrthoDB" id="6360546at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043014">
        <property type="term" value="F:alpha-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060285">
        <property type="term" value="P:cilium-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.170:FF:000002">
        <property type="entry name" value="EF-hand domain (C-terminal) containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.170:FF:000008">
        <property type="entry name" value="EF-hand domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.170:FF:000017">
        <property type="entry name" value="Protofilament ribbon protein of flagellar microtubules"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006602">
        <property type="entry name" value="DM10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040193">
        <property type="entry name" value="EFHC1/EFHC2/EFHB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12086">
        <property type="entry name" value="EF-HAND DOMAIN C-TERMINAL CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12086:SF9">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06565">
        <property type="entry name" value="DM10_dom"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00676">
        <property type="entry name" value="DM10"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51336">
        <property type="entry name" value="DM10"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="DM10" evidence="8">
        <location>
            <begin position="91"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="DM10" evidence="8">
        <location>
            <begin position="254"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="DM10" evidence="8">
        <location>
            <begin position="422"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="546"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8207884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="616" mass="72908" checksum="7D7261F43E760A2C" modified="2022-10-12" version="1">MSLNSQFPKQVPFLPGHVFSDPFKENHHKTQQFSHINGTVQEKTNFIHEEFDPNLMDSMKFNAPPNLTYGSRQPPENDYIPRIQPPWLKYDRAVLRFYGYFQESVVENPNENFRIRKVIIYYYLSDGTIHVTEPRVPNAGIPQGLFIKRQKITKKLGKQDFYKWEDFQLCSNINFYERVFRIQDCDTFTREFYEYMGKPLNLPEQMPRDNFENIRETKELKINPPDTKEYKEYFEVKLGGGHPNGGLNKYLQNDRKVLSFDIIWDDASIEGQLNYYTLNFYLADDTCEVKEVRKQNSGKDPYPLMLRRQKIPKAPILTHYPGMTLKKEEFYSPPDLICGNTVKIYGRECFIYGCDDFTKEYYLNVLGIQQKPATLKQERGKKFYQPVPPYNGYGYEEDSLGSVYSLQPKPPKKDVRKNFTQDQFILRFEARQISEVREENSRRFIISFYCGDDTVQVYQTSERNSGIWGGKFQERSRQKNPLTNDYYTEKDFQLGGIVQFNVYKFQLMRADEFTINYMKQKSDVFKEADISQIIAKLRLFADGYPNFDSFLLDLMKKLDKQLKGYIDFEELSQGLQELGFNLTLQEQYFLMREFDRNGEWKLCMQSLWEALGGKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8V9</accession>
    <name>A0A8S1Y8V9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Leucine Rich Repeat family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1490056</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000149">
        <property type="protein sequence ID" value="CAD8207964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y8V9"/>
    <dbReference type="OrthoDB" id="287501at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052394">
        <property type="entry name" value="LRR-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8207964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1079" mass="126565" checksum="8857E0B14538D9B6" modified="2022-10-12" version="1">MKDDYSELTTLGTSSSSAYRRLVFLQKSDVSELYTDLTNERNAQQKNKKQLFQKRFQCVQEQNESFEAQDQLLKMEINDWQGFLEKEHNAIQQINNEDITKKEIKSPLTLKMKTNPSMVSPTNDRTNYFYKMKLGNKQLSVINTQDFLKTVRYSQKFEDYSKLYKQQPHNLYLSLNFSQPERVPKTFGLIQNTRSLVTANASRSIKNSEDGNAFSNAMLTQQSRQIQAMQLNHNQFTPKQLRDILLTFPITLKDLELQNCKLNYMHMEILMSFVGKNQIYKLNLECNNIRDLGTQIIMKHLLNNNTLQSLNLNNNQITECSSTSISNLLKQTQRLLEFYLGYNYFNASAGTTIWKAMYKNTSVKILDLSHNNIASVECAQSINKAVSRPYNELLHVDLSFNKFTYPQAQIIAEALQKNETIYGFHFEGNQAELCVNANGFLINNIVYHSQLKDKLIQLYKQPQYFKLLEEAKVEQTQIRREESLVMPFSRSRKIRSTKLNVQKVNEINKLDTCWICEGWQEIKFEWIAHKSGSLYNEPIFIHFDFEDYRPLLMTFLNNEFFFIKMCPPNKEIHYFFTNPILGVQQPAMDQNIKNMQIQSIPFLYNDEILVDGNVMDQVNVLRTTNKQQLFDKYMPMVQCKPREPMAKFDFSPYLNIKKHRWSVESSIFKHFQPDTPQLIDECFEFDFQNSKVTRLVKDTELLEIKENLKILYRQLFHIYKYHASGSLGSPIPCIMIQDYIDFLVQTSLMDGYKTNDIDISFTSTTAAKDVQFPQAFDKGLVRCQLLEIMIRMCNDKYLRQGICTTMTEAITLVTKQAQDYFAKFDQAQMWRKSRLWNQKCDIILNDRLAMLKSLFKYICKLSKKDKLVCKYDYISVQDFKDWIVQSKLICDDLSERECYLIYLQSMITQKDELYSSKHYQMNFHEFIESVGRLAEKLSIIRGDKPMDIDDRRAKDLTSKLDGFLLYVYLVIGNDMKQALPPNDPDIRGVDKCMINDFKSLKQKLEDSFTDGDEPPYDPRVELPHLSSQITNLLGNTIGGKKQTLRQQLKQVKREEQKFSPINFVQYFKSIQQVHVDHDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YEW5</accession>
    <name>A0A8S1YEW5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1660108</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000166">
        <property type="protein sequence ID" value="CAD8212141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YEW5"/>
    <dbReference type="OrthoDB" id="294093at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8212141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="114" mass="13695" checksum="CFA4A310597DFC39" modified="2022-10-12" version="1">MGAQFRLRSLQQENMCLESTRKQCQTRRNANLKKRFQTEQMTVLSKIELSHNGKDQVELIYESYPVIQERITRTNKKVQQRLNVQSPQFKMYQQTSKTISFHQVKRILQTYINQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RVZ1</accession>
    <name>A0A8S1RVZ1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0010465</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RVZ1"/>
    <dbReference type="OrthoDB" id="7933078at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006214">
        <property type="entry name" value="Bax_inhibitor_1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291">
        <property type="entry name" value="BAX INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291:SF47">
        <property type="entry name" value="TRANSMEMBRANE BAX INHIBITOR MOTIF CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01027">
        <property type="entry name" value="Bax1-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="223"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8132146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="252" mass="28621" checksum="657F047F08D8791D" modified="2022-10-12" version="1">MQQRDEETQRILEKNDHYNEAPEVDSSLTSRLGFIQKVYSILSMQLLFTALLTIVSITQPAIREFEVTQIWLFVIAAITAIVLMCCLLCCKQNARKAPKNYILLSFFTLCEAYVVSVICCSVATDYSNGIDLIIIALSMTVLMTMGLTMYACTTKEDFTICTGLLWSLAICLLMLFIFSLLFPSRLIQIIYSILAIFLYSIYIIVDTQLIVGSKRHSLQKDDYIIGALILYIDIIILFLELLKLLVQIFGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RZ39</accession>
    <name>A0A8S1RZ39_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0010249</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="300038at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="256"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="882"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1101"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1200"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1404"/>
            <end position="1423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1443"/>
            <end position="1467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8131714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1498" mass="178850" checksum="C1480E4C5A4FD581" modified="2022-10-12" version="1">MMSQTINKLFYLSAIADHDRNDNLRSSWNKIKIIIYFIQVISLLFIDNINIYFDNYILILAQFITFQQIQNLYYQTLVLIAILLIHIIILLMISLLLKIKSKILLNTLQFYLSNYDWLFLIPSQIFCFMNKQFLITVLSILVLFLSVFQYFISIYIFRNSGFKTSNGRAKNITFDLIYLFIFESIIFIRMANLEITSIIKYFLVIAIILVKILEFIKIDKFSDKTVQSFYQKFIIITFVFIVQEILIQLLDYKQDPYLYTILLLFPLLNFFYVVEQFYILDAEYFYSNAIQKFDPQCQIKAYQQISYHQKKCQNIICPCKTFIRLDNKQEIIKFTVQILKLEQQFCYLKYISFQIQNNNKGFKNYLQIMKVVKQQKDIEFSFYDQKMIQSLLEIVCEQTKQLYKEIIPNKFSEVVYSLQWFESNCHKIKDQLLILLNIKLRLWSLYVTDKIESYQEMFEMQLKITYEINKIRQALHEFQQDIQVFLNQEINLKHNLFYQRVSQILELSFYSINKYQLIEKRIQKIVEFEQGQGDLLNYQMFENQKAMQLLVVISKQQRGKIEKVSNKWLNIDKFEHLSNIMPIQFNELHNQLLDNYCRFGPSKSQIFQTFIKYGDTPLVSKVKICLNNYPKYDHEKLYLMGTLVKDNIEEDQDYMLISQDFQILGYSEQLWFKIFQHLKFQDDIYEFSIFQILPDIKSILKEHYENMLRYNLQFQNHEKIISHQQKGVLYLNKNMCKQIFNSTTKIPFLQLSPQERIQKIEQLSKHYDNQEKSEFQLNLMFSLTENLITYNVKEKQVNYIYYWITFNFQETNTLIDKQFNQQLSYEVQVLEDNNKIKKIQFNQIKINESVAKTSSSMQKSNSEILSFQQVIGIVKQLTAYRLLMLLSAAYILLIIIAFILLFQQIQKKELQQTQCFEYLDYSTSFLDGYGRAMIASRHVIYLRDFNKFLASYNLYPIDSKTIINVTSVDKISFSLYIYKQSIEELVAFYQKETAKLKIYDKQNQSVDLIRINFYKNQTEIQTILSLSQYYKIMLQTLFMAIKSFAKDPTLYLIGTTDSWAQVNTRSILYFNFKRVANITLNFMRQCQIDDQQINKNLDFNFNVLLILIYSMILLQIILFVILYSAIKKKQRFVLSLFCRTSLNEGIEEIAFIQKLLNIVKNSKLWFGLYEYDEEDENKITRSYSGRAMKSHISHHLSSRVLLKLIIPLIIISYGSFLTLHMNYIDYSINLSPIVTAALKTQYIRLLFLDTINNWDVYVNKKFYEAMIAIQPTASGRYVITSQNIKHGYQLLRLTNVDVDTLKANLQELQSEPFSHMIYSQTLSVNSDSLLGQDICLMEIFNCNMNETVYKERVHYQQMYSIYEVGIINLFKQSISVMNQNDFFSLTENYEIINAFERLEKSQDYFFYFLWGFDAFLFQMKLFSEFFIDLSKTQLQDLSFNIYIIFYLFSITTTISLMMILLFGNIAIQQEFIHTLEMLYQIPKQTIINKQLGRQIKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S279</accession>
    <name>A0A8S1S279_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0030250</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S279"/>
    <dbReference type="OrthoDB" id="306940at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8134328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="179" mass="21009" checksum="9B57C76BECD62855" modified="2022-10-12" version="1">MGQKNQKATPVQTREPIEIKVDLKRIWRLLDTEFKINQAKQALKYNDDQVAKQFLQIALNKIKVSLNFLRMANRVLFLEQEVKSTQANADNLVNVLIECCYTNSVIICEQSQQNINQIDRTLDDIMVQGKVMDPMMSKYEDNFQTNMTVDYMIQQLKKEEAQQLQELQPSNIQYKYIQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SAN8</accession>
    <name>A0A8S1SAN8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Casein kinase I</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0050176</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SAN8"/>
    <dbReference type="OrthoDB" id="295705at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001472">
        <property type="entry name" value="Protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="401"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="401"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8136490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="434" mass="50054" checksum="BB2C652118343B2B" modified="2022-10-12" version="1">MLKNDKPVIIDDQYLLTSIYYTGRNSVYYNAVLIKDDQKSADILVQIVTNQSQCYCRLYKKTSDNGITPNIHQFGILKVGETSYHYIVQQKAGPSLKLCFQLMKRKFSIQTIALIALKMITHLSQLHSLNIIHRCLKLSNLITTLSSDIYFTHFEYSSKYMDKNGKILLHNKNKLNLYINKFSSIGTHLKQFPCPRDDLESLFYVLLHIITSGKFLPSQPNVKRQEKLKYYYELKQRFVPERELKGFPEQFLQLYHAIRLLSPHEFPNYDLLKQPFFQLLGQNDLQLQFDWIHLLKPKHKNSTSTSPVRISKLKHAGSVDNMAFIQQIKKQDSITDTVSPDRQGKQTMKHFAFPTSRNTTQHQLPAIQERQEGTLNSINQQNQYKKKFGGLSIVICKQQDTSSSSSGNVSSMSDDTSEVDTKPLNGVIGKLDFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SFS8</accession>
    <name>A0A8S1SFS8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0060341</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SFS8"/>
    <dbReference type="OrthoDB" id="309983at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38019">
        <property type="entry name" value="KDA ANTIGEN P200, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38019:SF1">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="577"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="28"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="598"/>
            <end position="607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8137882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="607" mass="74858" checksum="7345C5CEBE718C3D" modified="2022-10-12" version="1">MENNQEGNNYNETEEEHQQNEPQETEKNVQQQNSNVKQQDNSPQKQNRDQQNNKQQQESNPEGEEQFDEENIDANSFTPRMKEACKREGFKKEDLIEKGKDLIIYMLKQKDPSGQLANEDVIQMVFDHFEEKRRQKIQLVRQMRQKILQEESQGLWPNQMSNSSVFRSTSSLAEKREKLLSSIRKRQELEIEKIINSEQKMEELTRNNAEKEKRIQEKEKQRELKIQWQKEQAEKKRLQLEEERRKKEEEEKRIQLELEEQYKIKDQKRKQREEELERRRKEENKFKEEQRIEKQKKIQQDREQFLQRQQEIQDIRLKELQEKDLRRSQKFEEERLKRIERAEQERLEQEAKLEQVRRLNEEQLNKKRLDYEEKQKFVEQQRQYFETQQQEYKKQKEKEGEIKQEEINKILKQNEEQMRKKIQDYEKKQQELQLKKKKQEEEQKSLQLEKQKLFQEKERKIHEVLIRNEQQAEQHKNELLKKLDQAKVKCERIEKEKQEELTRKLAIAFIKDLDKQDNLQRREQLDELRKEQLEQRIKQDLIKSQQIQVEKEHLIKMSEQLRKEAEEKRQQQLLEIEKEKKKKHSMNSHRIITDMSKSKLSNRAGQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SLK5</accession>
    <name>A0A8S1SLK5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0090022</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SLK5"/>
    <dbReference type="OrthoDB" id="298557at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8140336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="446" mass="53307" checksum="8C868E8C9B0D40BF" modified="2022-10-12" version="1">MKIFWLRPSAGDSQQSTVVYQKQQQETVKKVITPQRESTKFPFHLSLENAKFIIENVNNKILSSQVRALLSAPVKNWMQLNTNLKNLENCNDFFNIHVNSLFIQLVEQIFKKQNENYQMKTLILTLDYYMNIQTFLLFLYWKLLRNNEELILIEKILDWIIKIRYICLNIDYLMEHFIHFLFDNRIQLRQVKPYEIIPKPLIGYEEMDLFQIDPVVLSNAITEINEEFYSQLQFNNLLTNLNNGKQSQFLSEYFDRINSFSLYIQATILRKKDRKKAIDYFYEVCLELCKNEDGEGVFLLFTLSIERLDQDLLHSIQYISPIQQQILIDMTKTFVCNKHIYTPTYTKKLYPIPSFYHFWTNLKKLEQAAQQSKDLKYIDQIRSILLDLMFIRESNSSRLLTRQKQNDPLLKYFLLKGHQTELSQILEIPIGNWNQMQLKLMKMASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SLR0</accession>
    <name>A0A8S1SLR0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA (guanine(9)-N(1))-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.221</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0100091</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>guanosine(9) in tRNA + S-adenosyl-L-methionine = N(1)-methylguanosine(9) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43156"/>
            <dbReference type="Rhea" id="RHEA-COMP:10367"/>
            <dbReference type="Rhea" id="RHEA-COMP:10368"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.221"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.221" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SLR0"/>
    <dbReference type="OrthoDB" id="278300at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052905">
        <property type="term" value="F:tRNA (guanosine(9)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18089">
        <property type="entry name" value="SPOUT_Trm10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028564">
        <property type="entry name" value="MT_TRM10-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007356">
        <property type="entry name" value="tRNA_m1G_MeTrfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13563">
        <property type="entry name" value="TRNA (GUANINE-9-) METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13563:SF13">
        <property type="entry name" value="TRNA METHYLTRANSFERASE 10 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51675">
        <property type="entry name" value="SAM_MT_TRM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM-dependent MTase TRM10-type" evidence="4">
        <location>
            <begin position="67"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00048434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8141393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="252" mass="29835" checksum="AA43E76B0A89FF5E" modified="2022-10-12" version="1">MDQQQGENKLSKKQQKKLAKKEKQKEYFKEKKIQKKQIRKQQKQEQKSNIVVDDDNKIHNQERQHKKPREQYQEEMKNGIKIIIDLQFMSQMTIHEKTSLYKQIELCHSNNFKSKKPLNLIVTSLTEDFKQLLNKSNASNWGIELNEKPYIDLFKKEDLVYLTGDTEDVIDELNQEEVYIIGGLVDHNRLKLITYNKAIEQGIKTKKLPINLNLKTSSILTVNQVFEILLRRNNGEDWNDSVQNTIPKRKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SSE4</accession>
    <name>A0A8S1SSE4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0120061</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATG3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SSE4"/>
    <dbReference type="OrthoDB" id="1584384at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019776">
        <property type="term" value="F:Atg8-family ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061723">
        <property type="term" value="P:glycophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044804">
        <property type="term" value="P:nucleophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007135">
        <property type="entry name" value="Atg3/Atg10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12866">
        <property type="entry name" value="UBIQUITIN-LIKE-CONJUGATING ENZYME ATG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12866:SF2">
        <property type="entry name" value="UBIQUITIN-LIKE-CONJUGATING ENZYME ATG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03987">
        <property type="entry name" value="Autophagy_act_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8143143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="229" mass="26480" checksum="B4D63CF91E7CA041" modified="2022-10-12" version="1">MFQGINNFVNSFATPKPQDFYSKGWLTPEQFVEAGDQLTMTGWQWKKAQVKKGVDPPHPEKMYLIANATSQTRIKEFLSFDFQNNQGQEGFLCVDMSKKQQQALNDQETRVYTITITYDRKYHCPRLWLQGVALNSGLPLKNQEIYEDIMSVYQNETVTVEEHPYLHYQQVTIHPCNHSTTMKAFLDKAKQNGADIKPMQALFIFLKFMQSVMPTVIYDTTIDICLGVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SSL0</accession>
    <name>A0A8S1SSL0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0110392</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SSL0"/>
    <dbReference type="OrthoDB" id="289015at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000143">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="56"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8142898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="346" mass="40532" checksum="23B68695AC2819B9" modified="2022-10-12" version="1">MSILVRKTNKQMGSLKSFRTTQQKQKLPQVSKYRYTSTKFHPLKTEPDFINDCVKFFTIRTLQGIDDNIDNNQDCIFYSTNYNDNEDQYLFGICDGHGPDGHLVSNYVAHQYKRKLRISLFFGDHQSQNIKLMNQCTIDIEKEMQFNDEFNLMLSGTTLITIFINGDKIICQNIGNSKAVFFSKSLQQWQHKELSQEHKLSHNQECFRILKSGGQIRQLRNEFTNKRQGPYYVFLQDQNIPQLYSTRSLGDSIGKKIGVTSESEFQTFQIKQEGFLIIGTCGLWDVMSYQEIIAQLDQIRLITQQKQIEELANSILNYCKRKWETYYNNNEVIKIEDISFILIYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1STL9</accession>
    <name>A0A8S1STL9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0110316</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1STL9"/>
    <dbReference type="OrthoDB" id="309494at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="81"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8142746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="211" mass="25516" checksum="AA779F679D2BDD5F" modified="2022-10-12" version="1">MSLGIDFERYQFDRRLKDLQDKILSLNYRAPQEQVPQVLPVSEPQYLNNQYYPKPIYYSPQLLYEYSQNKNAYTPYFQYQIDNKSQKKEKEEKRRVKELERKYEIQKVQENLAKQQELMMASLKKDILDYIDPIIYGNYRSYQNYVPQYQQQYQILTPQTLNQVPLYLQATNPSQVFSDDNLRNSQSYRSSFQQVGQNSLNQYDHHLSTRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SYY1</accession>
    <name>A0A8S1SYY1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0140190</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SYY1"/>
    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052276">
        <property type="entry name" value="Diphthamide-biosynth_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44240">
        <property type="entry name" value="DNAJ DOMAIN (PROKARYOTIC HEAT SHOCK PROTEIN)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44240:SF10">
        <property type="entry name" value="J DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="20"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8145333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="206" mass="24690" checksum="E5A616CEB9BF495E" modified="2022-10-12" version="1">MLLRKSINYFSIFTNKIPKTYYEVLEVTPKATIKEIKLQYIKLVKLYHPDNGESGSEEKFKEISKAYQVLKDPIKRQLYDSDALNFEQSGEAHSANDMNPDSYFYNTNKREYYQNKWYNFRKPSYETQHEEQTYQFVDELTSKAIIGRVLIVLGVYAIWDLYRLYSRRQHKKFLETQKEILDSSFIQAPLEQYIDTRQTELQLDLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T1U7</accession>
    <name>A0A8S1T1U7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0150297</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lin-54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T1U7"/>
    <dbReference type="OrthoDB" id="293367at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005172">
        <property type="entry name" value="CRC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028307">
        <property type="entry name" value="Lin-54_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446:SF34">
        <property type="entry name" value="PROTEIN LIN-54 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446">
        <property type="entry name" value="TESMIN/TSO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03638">
        <property type="entry name" value="TCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51634">
        <property type="entry name" value="CRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRC" evidence="5">
        <location>
            <begin position="123"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8146343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="298" mass="34576" checksum="F014B93563CBBE34" modified="2022-10-12" version="1">MNTRASKKQSLPLHIPPETRTKQEELIGEPVITKVNIDNEESNNKITSLNFRYNQGIKKLQITKTYPNEKSGFKAHKTRNKPIVLNLLNCLKQGVDGKLYDVMDVNTYIQFMNDQQQSQNIPKSLKCKCNKSMCLKQYCDCFANGNMCTTQCQCQGCHNTEEYLEERDEAINKLKMQNQSIEKEVPIGISCKCKKSKCLKRYCDCFQNYQKCNETCQCFNCSNQLEKVEQGDQRLMMNSISQFDENSRLAKSPIVYNRQGLFRRADSMNYSNSFGYSRRMLIQHEGPYILKQDSQGFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T5I5</accession>
    <name>A0A8S1T5I5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0170095</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T5I5"/>
    <dbReference type="OrthoDB" id="294318at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8147700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="429" mass="51509" checksum="E6E1921BE2D0FD64" modified="2022-10-12" version="1">MLPYLQKERSNSFNVRKPTERYSIHADVTPLGEGRGVFIILILNNEEFIGELIQNPHYLQFLRYKEFLNEMQIKQFVVYLQEQLIQSTSKSHTQHYCTFSQQSKKIDLLNTSSREIYEVLFEFAERINKDKQFNYVFGVDFNMVHKSKFKSILFLSETRIDLSIMELIQPQKITRGRTFSHQSVIQTSKEQSEILDTDNKYQQLASNKRSKEIKQYLKEQNKLKQETKNKDQFMIKKIALTEKIEPQKQQTELPQKCKQVLQGFQLIFGIKTINMDRDLFSQFLLLCEMTILYTTNKMLQISIHKIEESKRLEVILKQLFFDYKILKSMNIKLQQLNPTLTRCLLRFLKQGLEYDRQNRLFWDDFLRFNQYYIDRIWFVSDFRLFLMLLNDENQSFTSKIMKVQPLNIQRTILNLEPESLQNIVYSLIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T6K7</accession>
    <name>A0A8S1T6K7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0150461</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T6K7"/>
    <dbReference type="OrthoDB" id="10049342at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8146644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="628" mass="73421" checksum="D6CF83435B10E232" modified="2022-10-12" version="1">MANQLVLSRQVSEQQIMDLQIQAITQMCNFHKMRLGTIIHSMHQKSSQFIEESLQLVEQEVQNHSYWSLTSQTIKKVNQNIENLEARLKNEINIEKNNLSYQIKLISEQIREFIQNIISIINQVTVSTTEITEKLFRILKEIEELLFRMEMVINSNRNSLSHFNISLAISVILLEVSCEWAFGKKMRNILKGVTEIALPFIFAANANKKLQRQFNDLKCLFEQQKRLIDQNVTKSIFEMNIAMDGVIKTFLTNIKRPNLNNQRAIAWLDSHIENYGNSQLAQRIRNCFPNIQHCHFTNDHQSLINFLNENYITFLIIQGSIANETLHMLLTYPNIHSILIYCQFPEKYEDLIQKFKQIVFITNQQDEVIAALKKSLAPSLILREINLYQFPNYFQQNAALYYLSEEFSLSSIPQLSKDEALKIIKDTMQYVPNTVYTLSEEAQVELALQIHEKFKNGQIEDIINLYTGPSGFFKFINRILQSLNEKAILEASVIIQSLKYSLEKYQDNIDPNLFISSEFFLYRGVDCTIEAFKLKHKLYDLMMFPAFTSTSRDFAISKSYAKDLGIIFRISFNENNLDDSHFQMVRPKAIYTVSQFQNEQEYLFSCFSCFIITKFNEDNIIDIEFVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T7G8</accession>
    <name>A0A8S1T7G8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0160380</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T7G8"/>
    <dbReference type="OrthoDB" id="354826at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05578">
        <property type="entry name" value="STKc_Yank1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000454">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000654">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF374">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="23"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="11">
        <location>
            <begin position="282"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="382"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="382"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8147419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="428" mass="49647" checksum="2E1BBBED70C0EBCA" modified="2022-10-12" version="1">MGNCNNSKDVNFDDVSLPSKNDFHMLFVIGRGGFGRVWKTENKKTKQLYAIKEMSKCKIVNKKSVSSVMNEKYLLSNLRHPFLVNMHASFQDRENLYLVMDLMQGGDLRYHLCKQKRFTEKQTKFFIVCLLMALDYLHTNTILHRDVKPENLVFDRNGYLRLTDLGIARIWKPDNENDTSGTPGYMAPEVMCRQAHGVASDYFAVGVIAYECMFGKRPYVGKTRREIRDQILAKQVLVKLNQIPDGWSEESADFINRSLQRKPSNRLGANGPEEVQNHPWFKDIDWTSFFNQTVVSPYQINYNHDNFDAKFANMREEEDNQTIQQNGLMLRRQSVQDLFNGYTFDQTITQVPFQNTSVTKPTIPHLNTQQLTSARRTNLEDTNPQLSQMSSKKYHLVSSTPRLQTKSMSSSQKPQTDRSHFSNKFQFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T8P1</accession>
    <name>A0A8S1T8P1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0170333</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8148168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T8P1"/>
    <dbReference type="OrthoDB" id="292444at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8148168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="116" mass="13867" checksum="79E7C008CFEB2D02" modified="2022-10-12" version="1">MQFIDYNFKHVFMCQNKRLWILYTQMRILYSSNNERVLINKGSFSEVLRFQKPIQKSDYNLLCKNTNNIAMYTNQCVLYSIFNQNQTLLGILEISISVNDYFSFDEEYYGVILAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TDG3</accession>
    <name>A0A8S1TDG3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0190367</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TDG3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8149893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="115" mass="13231" checksum="A6D1491EB87C6CC7" modified="2022-10-12" version="1">MKREIILTIKGKEFGSMFKKIERLEKDNIISQEKRMENGFSRAQDIGISPKSLIVAFIKMEKKLANGISCIGRKGRNSFNIEEVGDNMMNKALRKLENGFTEISQFIQQCHLSNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TH55</accession>
    <name>A0A8S1TH55_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0200232</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TH55"/>
    <dbReference type="OrthoDB" id="9984778at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053238">
        <property type="entry name" value="RING-H2_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14155:SF610">
        <property type="entry name" value="OS01G0755700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14155">
        <property type="entry name" value="RING FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="311"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="382"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8150382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="486" mass="56366" checksum="C68B94BEEBC1F6DB" modified="2022-10-12" version="1">MITEFSKHINYLVQKNQFKMIILIFLTCIQLSVNQKIEQNYLRVFGIQVFQIRNITKNGFPQKSKITIGLSHLDGEKPILLLCSNKPNANISMNYDSIIKEQCWYDVNAYEDKNKKQMFSLQDKLIVGRYHHIFNIYEFQDTGLFIGAISKSQSSYSIQAEIQSIYSCSKECHNGGSCFYGVCECLQGSFGDDCSIQGLNILEQKTLSSKYLYYLNIKQLTRTTFLLLLSNSIPLRKQCYAEKPYVNHGSQVLTNMIQLDHDQIKNCQNITYQVQDELKVQQIPYYLFKIIDVNNVEIFDNNIQEEGISSIMLLLIIILSIILFLIIACCCSKVFKNKIDLSRQQLQTEPIPTYVDLYMPTYKFQEIKDSDIPYIHTDGQYCSICLERFTLLNNVKITYCKHLYHSKCLRLWIEKIKICPLCRAPLDEQTIISMIPPKSLTLIDQIINKSTNKFKSSQGSLNSLNNQNANKFQNLNYQRSLAYIDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TNS3</accession>
    <name>A0A8S1TNS3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0240329</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TNS3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8153643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="92" mass="10989" checksum="F4FA10818D241B87" modified="2022-10-12" version="1">MELAQGPLTIHSQISMMHQEPLVYLSIKNMQLVIYPFLQHSNNISQQHFINLVGMNQQINVFHEKLSITLSMKNYQSKYQYRQQWINLGYYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TSL0</accession>
    <name>A0A8S1TSL0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0250100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8153926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TSL0"/>
    <dbReference type="OrthoDB" id="304936at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8153926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="175" mass="20870" checksum="6B70559038FC91E6" modified="2022-10-12" version="1">MGSACQKPPDDRSYYQETEEIQQDDFIIEQEQYPLKIHKGGRKALHKSKRLQQEIESDDISQEPVIQSDDQINQMQIYKTYHQKSTYQSTEKNRIPQEKQKQLISIMKQKFSQKLTQSSLQVLSTTNSKLQQSSIKIKDQQEKHVRFKLPKSHKQKQRTHSNFRNRLNTQVYNYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TU61</accession>
    <name>A0A8S1TU61_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0260311</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8155097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TU61"/>
    <dbReference type="OrthoDB" id="546383at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005879">
        <property type="term" value="C:axonemal microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035082">
        <property type="term" value="P:axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026507">
        <property type="entry name" value="PIRC1/2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14892">
        <property type="entry name" value="PIRC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8155097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="88" mass="10467" checksum="A0E34AC7AC75C5DA" modified="2022-10-12" version="1">MNTNQSTNTKELSDEEFMKLHAKQFYRTHGQLIGSKQSYIPIPKEYNHNGKFTDHLLMATMYRNNSLNTTCDKERWINGSKDWMEHLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U180</accession>
    <name>A0A8S1U180_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0310186</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="295192at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002116">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013979">
        <property type="entry name" value="TIF_beta_prop-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF15">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08662">
        <property type="entry name" value="eIF2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25764">
        <property type="entry name" value="KIF21A_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Kinesin motor" evidence="9">
        <location>
            <begin position="14"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="1332"/>
            <end position="1364"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="417"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="468"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="560"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="778"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="953"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="92"/>
            <end position="99"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8158134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1393" mass="162578" checksum="FE224BB9113AA19A" modified="2022-10-12" version="1">MEVELHQQIPPQLNVQVAIRVRPFNEKELKQKEGVCLETSEKQVTLLQSARLFTFDYVFDQNACQDQIYKKCVSSLVQRCFEGYNSTILAYGQTGSGKTHTMGTTGIDQLSNKNNLGMIPRVITQIFEEIEKIDQEILISCSYLELYNEQIIDLLLETSISNQPTIREEKDHTITILNLTTILVNNPNEMLQVLNRGAAHRTTAATQMNMTSSRSHAIFTIYFKINPKDDSEDGTLNAKFHFVDLAGSERLKKTLAQGKTMEEGININQSLLVLGNVIKTLSDQKKKSQTHIPYRESKLTRILQDSLGACVSPAESNYEETLNTLKYASRAREIQNKPTQNRDPHAIQILSLKQEIATLIEQNKQFQELLQVNGVKFDQIKKVSISQYSSNIPHTCEEHVEIIQKQKQALLVWERLGSQHKLEVQKLQAQINDLEIELYNLKKKKDIIFKQFQDAKKILQKFNIPFTNYDEDDEIEDLYDEVDKLKQESKEKDYKILTMQKEIDELLQDAHRDQYILCKKQKELMILQRKIEKLDTKDNFESPIQDSECDEEIICNDSMIKETNQQIEEFESNLQVMTIRNFELKRQLAEDMKKEFQQQISMLESQKASLMKQVNQKQDATQLNQLKIKLQEYEQKIGEMRQKEQNMRQMQKKLEEQENQVKDLKINIEKMKKQKVELIKKMKSDNEKYIKDKEEKQKELILIKKLKIQQDAALSKLKNENCKKEVLLRRKEDEILKQRNEKVIEKGTSQSYKKSQKVQVYDQMEKQIESLFSQLISGGQAEIQIKREITKLEQLQEEISQIEQKICQLQIKREQTSFDQNNKNSLNQIELELSDIIIIRENINETIDYQLHKIEQLRKNCNNCTEIYTNFLTNSEFRELPNWCIRTFKFLIDNWIKDHLQMQNFSQQNDEFQQVIQELVQQLQKPLQEINCNSPTLKKKIVKQQISSQPNDALELRKQLNDQKRKYQFLQNENKNMISDLEYYKKFYTEHINKVQKDKCIGLSDQIQIPQSQSYQQIRDAYKLERTKQKELSLNLSFQKPQIQNSAIKIAKVCKSDREGYTDQFSQAQEEQELRYDVIKSIPGHENPILCVYTMQNLLCSSAFRSVKIWDMDAQSHIISLDANTHVKSICHWPERNAIAVSHGSQISLYDMTSFQLQSVLKSSIEEIRVMTRHNGLLVAGGKGINYAMNVWDARSNNQIHEFEKSSDVMCLQQSDQTQELIWGTFNHNVRKMMMNSNKYGQIAQMTPPHYDKVTGVGLLDNWIVSCSFGKGMRMWNQINGQQGQANSDIHKDSILTMAIDKTLKVMYTGCKDGQIKATRISENKFQLVSDISASVQQINSLNIINDSSLIVSGGQDRMIKIWKPSKFTYDYLKQFTPNIGDFIIEEDQYMFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U3E1</accession>
    <name>A0A8S1U3E1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0330277</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U3E1"/>
    <dbReference type="OrthoDB" id="290670at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8159695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="280" mass="33540" checksum="67B9315EA9A3CDC0" modified="2022-10-12" version="1">MSKEQNKFKHILLVPNIVSDQAFLQRHKTFQTYVKKAHSDLYYLQLKKIGGLRKSIAEKADYGQVLQQIKSNRLQNEYLNLQKKNQGFMYNNQLWSDKYYNQIYQMTRLSQLSEVLSKQKTKGRDQLIKDFEVYFNKHVHPFNDEFTEIQKVLPLSNCTTKCSSRINSQKFLFPTERLLNSKQETQEDDINRVKSLQTSFHKSINDKIVNLLDEAKTIKKTRQFQTEQSSSRKIIYSQIQRRNEHMNIQTSMRNRQIHFKNKFVQERFQKEIEKLTSQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U5K3</accession>
    <name>A0A8S1U5K3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sushi domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0310076</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8157936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U5K3"/>
    <dbReference type="OrthoDB" id="301543at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035733187" description="Sushi domain-containing protein" evidence="1">
        <location>
            <begin position="17"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8157936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="394" mass="44716" checksum="AE797F6E08E1396B" modified="2022-10-12" version="1">MKKFLILIASLSYAFTYTTTCIGISQALCTTAAGYCFWTGTACQMAECHKATDVRACRSSTPAGFLLSQTKCREIPNNDPNFLNQCMEHDQTAMMYAYVRFPQDITQLHSTKTTSGYLVTTIMTAKYTSQYKYEILTVNILTASTTELLNIMNAYVQMQYDLVDPEIHPQYLEKAIYESMQAIRDDVTLTLTGTTNKRQDYYTVLWRMTDAYFLKLRSYQPFYISNKYLINFGFSHFNRMSITLSSQFQSTKVSWTSYNDNGYLELLVIPAEQFGILNSFSDVIIIRPSNLDGSDATLSYTIQWSFSFLGTQPSSTSNIYTFDKIEMNTFISQGTANCNTSSKLCSKTFTNQSTTKAYLIGDPAVASQTNTGRYYTRCRLGRFNWVGSTNICNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U5U4</accession>
    <name>A0A8S1U5U4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">mRNA capping enzyme adenylation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0310101</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8157986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U5U4"/>
    <dbReference type="OrthoDB" id="200924at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004484">
        <property type="term" value="F:mRNA guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07895">
        <property type="entry name" value="Adenylation_mRNA_capping"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001339">
        <property type="entry name" value="mRNA_cap_enzyme_adenylation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051029">
        <property type="entry name" value="mRNA_Capping_Enz/RNA_Phosphat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10367">
        <property type="entry name" value="MRNA-CAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10367:SF17">
        <property type="entry name" value="MRNA-CAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01331">
        <property type="entry name" value="mRNA_cap_enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="mRNA capping enzyme adenylation" evidence="1">
        <location>
            <begin position="56"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8157986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="467" mass="55081" checksum="E17AABE4497DD63F" modified="2022-10-12" version="1">MEGYYENPGIFINQLLQQEDQSDFEEEFYWLQNALKNLNQQLRGTNVTGPEFIGAHPKSLSKAKLAQIKRELTDWLVCEKTDGVRYLLIILNNGHCYFTGRNLGGLNVSNSPYQLHLVKIRVPSQLINPQELKIIEMFDGELIVENYPNNTQALNYLIFDTLIHNTNYTSKFQYFDRLRCAQEYLELRKNLKKMGPLQNIDFQPLKHFPKIRCILKDFFYPDRARYICYNYIPLLPHGNDGLIFTKNSFPYVSGTNENIVKWKPPEKNTIDFLICPNRKIKVIDPNYGLLELYVMFNQKQTKNYFLFDFIYAKKELIEEIQTQILNQIEKLESLGSHEGVKGIVAECKLERLQSPVVKIQALYELNKNEYYDESRLENFYQQLLNNTELAFKLDSSMLQNQISLLKDIRERIHLKYNFKFQWVIEKYRTDKTSANALFVAQNLLEIISEDLKQIELIEILEKLNTLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U665</accession>
    <name>A0A8S1U665_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PPENT_87.1.T0340177</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8160124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U665"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000315">
        <property type="entry name" value="Calcium-dependent protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF32">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="119"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="417"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="434"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAD8160124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="448" mass="52089" checksum="97B591E9FB45CDF1" modified="2022-10-12" version="1">MNFQPSSLFNHNLKWELLNEKPSSDFKTLQLYVFSNKKKEFQFKTLIKTGSRILKQCKNNKIKYLDVKNCVLKKIHHYQNGDGIQITSGNFQFEFYGQITEWFEQLKLHCIQVDFLNNYNIGNLIGKGTYGKVYKAIQKQNKSEYAVKTFDKSNLQKQIEIDAILREIDILRIVQHPRVIKLLETYESETHIFVVYELLKGGELKQLMNEKKLNQDQCVSIIYKIIDALAYIHSKGIIHRDLKPENLIFRYAQNIEDIVIADFGLADFYRKDGQYLFARCGTAGYLAPELIMNKIYDYKVDVYSVGIVFYMLINGGKSPFEKIDYATQLQQNMLSNIDLKKLIITNDCQDLLSKMIDSNPQSRITSQQAKIHLLFHKQHEKIEISTARLPKSLTISLIDTPKIQSIQSSRQKKQSFVQISQSAKNSPIEIKKIQGQQNFSNAKQKQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U8I8</accession>
    <name>A0A8S1U8I8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0350132</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U8I8"/>
    <dbReference type="OrthoDB" id="300199at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="533"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="736"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="842"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1053"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="912"/>
            <end position="939"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8160714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1115" mass="132563" checksum="0D272E8F0A0342EA" modified="2022-10-12" version="1">MYILYYNIYTYLREESKQLLKARLRKNFCKQYDQTTLIFEEFYKTEYIIRILNLQMIKLLEDKLVFYNQLIKELISQNQIFDCCFKISIQLCQIEKNINQIYQQNPSISILNLKVFYQAEILRNYVQASREHSIIYELESQYKQTSIVLLAKQNKVAFLITKLGDCSYNIKIERASSNAKSFFQNELPQYIEPLIPQGIRDYHGQNLHDFFQTGSSPRYKQNARLFLLQNNYIQIAESYLDSYNSLSELRFINIILELKDIKGYMILNNQFQVVGINKQLLDLLNFSKSQIDYFQPDEMLYGLDVRLMIPQFLILIEENRAMSLIEFYFIKQCYIFDLTKHTMIERFTNMNLLDRFESQIEIQSKYHNDKLSYLIITFDYLKKQNITSLLSNFIECSTKSNQKIDLQQKLLEGIKIDSPYDFDDSKNLDIQPEFTYQQLNSPQEKSSTYELLFQQKFYNRYHDISGGSDDVSIIKKNFQKQVSMPQFYSENNKQKISVQDVCTPSKKKEKYDSNFQDKVEFIDKMGQKKIKNIFVRLAIFFLVVSSLLGIVSLLLQTFLIQNNFNNYITDVEQLSIKNDLLQPMLSFFILRFIIYDKTNLLNQKLITQEQYLNEIDFAKQYLKKEFDHFHNHIQELFESQLLQDLLYDHYYNVIYLQDDFTYTYENFSARTFFIKYFDYQQFIYAIYSNNELPKITTPVTFQMLNQKLLFEICSYLGEQVYTNTITRNSKQINDQIIVIACFLSVNFICLLFSNYYYRKFANQRQNFGSMMFNLEKSALESELENLDFLINQIENHNITQFYTFNEKYKEREIQALNQKSKTHYQNQHKQIHKKAKIDTTKFLIFIYTMFIGFLTVNLTNTLILSEFIQKYKPTATMHKNTSELCLNIALIYSQKEQLNNQVFFTYLRDSDFQNMVQQLNQSSIQIKQYQQELLNVNLNNLLFNADFVSYFLEQETQDLCPLINTNNNQQILTICMNSFEGSFLLGIQAVLNHVSKIVTQELMTQEFTQREPINTLEVEGAYLFVEYLQKASDVAMQDFSEQVLHYKSILETILISSISFTLILVATILSALEINLKLKLKYAVKFIHLIPREVLFLDNAFERKVRMAILKDPNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U8Y2</accession>
    <name>A0A8S1U8Y2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0340303</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8160372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U8Y2"/>
    <dbReference type="OrthoDB" id="302833at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="390"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="19"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="173"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="323"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="390"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="400"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8160372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="412" mass="48826" checksum="0AC373BC351AF2CD" modified="2022-10-12" version="1">MRRTSSQQNFEGRRDSNHLVGLMGENEKLHKQNESLMKELSLIKNDFERMSINGQKTVKNGRRKSCEITGEMVNQYQIKSLEEELQNTLLRFNTLKKEFSIKTNQYQNQITKQTNLISQLQNENEILKIKNENKENFEFKQSYQENYLEKSGIKIINENQKIKQLYESNIKLTKLLEDRLVQLEQLTKTLNQKNQEHMKEIEKYESLLKEKTHNSSLSQGKSFESLQKKIDILEKDKIRLNSELTKTRDLNSKEKQMLLKEKQNLNLEKEKLHSDMSIIQQQLTDYYNQLQQCGQQLKQKDESIKQFQQSESYQGYQNIQYENQQQKALITQLQIQIGNLQQTNKALHEELLYLKTTAPLSNRTNLKTYFGVDSPFKNIADEQNKYTVTLKTKTQSSQDQRGRSQEKYKKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UBA9</accession>
    <name>A0A8S1UBA9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0350274</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UBA9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8160992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="213" mass="25459" checksum="D124A65EF62C6147" modified="2022-10-12" version="1">MSLFTNSFRKLSQQQQENDSVKVYYKTDMKQYAKVRYNRGTTIGQIIEELIKELNLNGKLQYQIYLLTHKKNEPSLGGISIKRKLRRNEQPYKILFLTKVYKFSFYLDYMLSQLSQNIYDSHFNQIENYQFTIYDKEFKIYKITKKGSQKQMKIKINSSGTALCQEGNKKWKNLLNILIANLILKKILYLQLLKNKQTFIKLQIKMNFMLLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UBD6</accession>
    <name>A0A8S1UBD6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0360181</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UBD6"/>
    <dbReference type="OrthoDB" id="297040at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8161427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="124" mass="14734" checksum="21DF8DC1E9E4E24C" modified="2022-10-12" version="1">MSSSLESQILLAIWTMYQRNQISMEQKGCIKDLLIRKDNNLYQSICNCKRQDQIEERLLDILNYLDAEDNIYPIFRLSNQSSSPTTTQSQCHYPEKRFRFKSSNNESDTSQNFHRIRSNSQHHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UBN2</accession>
    <name>A0A8S1UBN2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0370280</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8162258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="288891at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8162258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="354" mass="43098" checksum="0961D28BB75EDF66" modified="2022-10-12" version="1">MWRFTKSQIADFVHYRQQQLPEGYTQNEEMNIINHYCCNLLPTLFKNEKNSQLTLISYAAVLFKRYYMIKTIFEQSGMHQMLLFCSYLGMKLNGLSQQSIAIQAQQLKKKPKHERFHHYGDEFYAKYEILIVQALGYDIDVKPIYPIIQSAIFIYQIKDLLEQSLFIRYEKKIEEYFLKSLQGFTIFTFKESEIALAIVECSLQSIFSEILSPHISLGGIKPLKQLTIEKIKKYYQEYKQNGTLIIEQIDQRDISIANHSQQIQEVVHLLQEIKDISREFEKNYXGRQRYLQSQFSFISQPLIYYYYYYYYYYYYYYYYYYYYYFSNLQYKIQDCLINIFELKQNIYIYIQQYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UC56</accession>
    <name>A0A8S1UC56_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0380027</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UC56"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="127"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8162420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="552" mass="64423" checksum="15909812A1765CF2" modified="2022-10-12" version="1">MTSEIHSFFDTKDQLKNKIWTKFCKNQYKNDFIEYQSECNRLSKTHKQLKPFSIQIGQETLYAFKNQQPQGMVQLTVTVMLFKRYDDQEFIRLQRNGQYVDIVIDNFEQLKKVLIGKCVLTTFHNDFNVLKMIGKGSFANVYLAKRQSNGVQYAVKAFSKLYIHGQFRGREGLENEIRVMRRLNQENILHLYEVHETQNSIYFVLDLLEGGELLKRAQSKIYSAQKIQQLMYNLIKALFHIHSKKCMHRDLKPENLLLKSKDNDTDIVIADFGLAHIMDQQPLYKRCGTPGFVAPEILKYNDGGPFYNEKCDIFSTGVIFYLMITGIQPFSGCEYQEILQSNKACQINYDIPQFNKGPQHLKNLLKKMLKADPDERYTAEECLKHEYFQEIYNQDDLLDNKENLQKYELEFISPLQRKNSQESQEGSMLLMSKQQQWNGQTETIGSLSNCSNVGSNNKADKQQTYLSKFSQFCSQMKQEGLVQNNQSIQKKKNRQDLHKFALQNSYQQKQRSKDDDYVIDDEQSGISNQLSQLNAQLPKMGFLKKSQSLDVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UCH8</accession>
    <name>A0A8S1UCH8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0380320</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UCH8"/>
    <dbReference type="OrthoDB" id="300211at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8162941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="160" mass="18983" checksum="D2CA7072F8E490C8" modified="2022-10-12" version="1">MLQRKIFVPSKRKQQKESLEQDDFYFESNFEAVEMEEITSMLAKRLKFENCEEGLNDSFKFKRCCFSDDKFISCNQGLEILTQQQEISLIPNEKHSTKQVNQLKCGEKRCIQLKNIAEFKHLRTGEINYLEFIGFPEAQKTLKFKKKKGNNKITKKIHKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UG76</accession>
    <name>A0A8S1UG76_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0400090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8163840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UG76"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8163840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="63" mass="7465" checksum="9F2BFB99AA829DDD" modified="2022-10-12" version="1">MQSQHTYEAIIKHYEQPIDIATIMCPEEYSSIIFQLCEVRNGKMVGYRDIQQIRLIYIQIPFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UKU5</accession>
    <name>A0A8S1UKU5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">LITAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0390119</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDIP1/LITAF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UKU5"/>
    <dbReference type="OrthoDB" id="306546at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006629">
        <property type="entry name" value="LITAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037519">
        <property type="entry name" value="LITAF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292:SF6">
        <property type="entry name" value="FI16602P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292">
        <property type="entry name" value="LIPOPOLYSACCHARIDE-INDUCED TUMOR NECROSIS FACTOR-ALPHA FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10601">
        <property type="entry name" value="zf-LITAF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00714">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51837">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="LITAF" evidence="7">
        <location>
            <begin position="60"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8163236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="150" mass="17544" checksum="536121610C666A4A" modified="2022-10-12" version="1">MEQNITCQAYPAFEEKQNKELPQQYQYPKEQNNIPVGYPNQQYIPIQQQQPFMSPQQVVLQPIVIQQVRRTNAEGCSYPTDILCPYCQKPVQTLIEYQSGCSTWLACLLLFLFFLPLFFLPFCFDECQDKIHKCPNCGKKVGKKKYKLCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UL44</accession>
    <name>A0A8S1UL44_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Opine dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0410028</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UL44"/>
    <dbReference type="OrthoDB" id="6058913at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051729">
        <property type="entry name" value="Opine/Lysopine_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003421">
        <property type="entry name" value="Opine_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38015">
        <property type="entry name" value="BLR6086 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38015:SF1">
        <property type="entry name" value="OPINE DEHYDROGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02317">
        <property type="entry name" value="Octopine_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Opine dehydrogenase" evidence="1">
        <location>
            <begin position="189"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8164429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="403" mass="45881" checksum="25A51DD81C48177A" modified="2022-10-12" version="1">MIPTSIVVCGGGNGSHATVASAGRFENIQVNVFTRKPQDWKQEIVGLTKGSPWESLGNFVGKINKVSNNPQDFVGAKLWIIGGPAHVHHEILQKIAPFVTKDSFVGTLYAQGGFEWMCRSVFGDRIRTDNITYFGLYNIPWLCKIFKYGESVRIIGPKTKLQCALSNLSRKEELFLLLENMFQIPVLQAPNFLTLTLTPSNQIIHPARVYAVFRNWDGKQVFQSSEVPTFYEDFDDFSAYMLQILDDEIQAIKREILKRYPHFNLDLIIPIKERIIQQYGDQVKDKSNLKTVFRTNAGYATIQIPTKPVQGGVQLNTEARIFWEDIPYGLCILKDLSEMLGLQTPGTDKMIEWHQKFMNKEYIQNSRLNRSLLKETGAPTKYGLNTIEKVLGIQEVSQQIPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UM89</accession>
    <name>A0A8S1UM89_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Actin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0440118</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UM89"/>
    <dbReference type="OrthoDB" id="6220758at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10210">
        <property type="entry name" value="ASKHA_NBD_Arp6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8166378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="386" mass="45604" checksum="45A6877E582E46D9" modified="2022-10-12" version="1">MDNWRQNNQRRFVLDNGSYQIRYGNDVQKHKQLNLVAHLKKTGQMIYDFDSLFDESQVMTYYKPHVRGVLVDFDKQINIWDKFLNPKLLKDCSITYPLQPFTPDQVVNKLFDVMFEYYNVDAFCPINAQKMLYYNVLAKEGVPRKQFTYIIDSSHSATYLVPIFDGEIMYSGLRRIDIGGRLLTNQLKDNISFKQFDLKNYFLLSSKIKEILCFVSLNYFQDMKKPKNHFRKYYILPDFEIRREGYAVDLLNVGFLDEKLILDKERMTIPEILFRPTEIGLAQKGPTETFFDSLSTIHKDLQGYFAENIYLSGGNSFFSSFYDRLYNEIRQNSSIDWNVNLTHLDDIDSVFQGSLHFNRSSEFQNASFTKAEYQEKGFDNIKQYII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1USA7</accession>
    <name>A0A8S1USA7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0460207</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8167057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1USA7"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001129">
        <property type="entry name" value="ras-related protein Rab-44 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="182"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8167057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="197" mass="22498" checksum="A50D67D330208CAD" modified="2022-10-12" version="1">MDPQQENFDQSVKILLIGDVCVGKTQLIYRYCENKFLNTYLSTIGVNYNEKSIDIEGKKILMKIWDSSGQERFKQYTLSGYKGASAIILVYSINDRGSFNNVITFWTNQIKQYASENVCILLIGNKIDESNRQVARDEGDAFARQFGFLFYETSAKEGTNVTDAFYAIVKQITEKQPIPSAEKQHNPPAEKKQCNIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UT98</accession>
    <name>A0A8S1UT98_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0480098</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000048">
        <property type="protein sequence ID" value="CAD8167921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UT98"/>
    <dbReference type="OrthoDB" id="285560at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8167921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="85" mass="10112" checksum="837F97AB427087A4" modified="2022-10-12" version="1">MIKTRTKPVCKTHFNLSSKQISKYRKYILNLVNRKWLKQTDNVSTDKCKIKFIENKIHHFVPTDTIIQKVIKFDCYPKKGILKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UVD7</accession>
    <name>A0A8S1UVD7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0480069</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000048">
        <property type="protein sequence ID" value="CAD8167882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="308122at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00106">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000116">
        <property type="entry name" value="Putative kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044986">
        <property type="entry name" value="KIF15/KIN-12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37739">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-12D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37739:SF8">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-12D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="9">
        <location>
            <begin position="11"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1687"/>
            <end position="1710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1826"/>
            <end position="1850"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="343"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="584"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="859"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="988"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1076"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1242"/>
            <end position="1639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1700"/>
            <end position="1709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1827"/>
            <end position="1850"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="93"/>
            <end position="100"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8167882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1850" mass="219148" checksum="9C04FD02E66CDCE4" modified="2022-10-12" version="1">MTDKFKAERDNIQVSIRIKPLIEDGRSCIKLDSNLKNTIIIEGNQQQQQDQKYFYFDYVAQQDSSQEDIFNIVGKQQAINCLDGYNGCVFVYGQTGSGKTYTMMGTQKQPGLLPRVIDFLFNCILEDSQENVEYLVKCSYLEIYNEHIIDLLNPQLGNLQLREDLKKGVYVEQLSEEVCTNVTESLEVLQRGSLNRHISSTQMNIESSRSHSVFTIQLESRRQSSQTQVINHRFSRFHFVDLAGSERQKQSQVQGERLREGCNINKSLHILGNVINSLVEDNQSYVHYRDSKLTFLLKDSLGGNSRTHLIANIQQSQQFYQETLSTLMFSKRVKQVKNKARINEDESGNLESLKNEIKRLKQELAKSVTTQQQKWESPKKFEQKQQQQSFINEQFLQSLELESLMINDQKYIKLEEILKCYLEQSTESETALFLEIEKYLNGIKQLKEGFQLGQQLEQQLKFVIKLQNEQIQKLKLNQSADDIQQQIQQQLAYALQSQGLVMKTFYENLTLKEQQGATQTVSKVQVQVDTNLNLLKDIVETVQNSLNERRQFQQQLDLQFNSQYVTLDKFKELETQSEYKEKLLSEQTQKLVQMEEQIKEIEKQIFHKLEENGITFNENDQIVDVRDQQIQDLKFKITDLNQELKNSNQELEELYNNSEKVIQQLKIENQQILTQKQEIEINYELTQTNISVFQDNSKQQIAILEQQLIQLKDQNQQQSNQYQYLNQQNEEFKQQILNQQNQNNQITQELIENEEKLKQLNQIIVDNDITIQELKQQLSTYESIITQLKNNLNDQNTKLQLNQQSISQLEQENINIKQECSKYQNQLVIIQDQLQLSMSKLENSEQELFTQLQQSQIDNQNLQHQNSLISKEKQDMQQEIIILIQKLNSKDEDLQNTHLQLQQTQQKYQEEIAIYQQNQLVQNQNLEKNYQDQIKQQNKDFELLLSQLNAKKDQDQTKLNQEIHSLNCIIKEKETYITETQSQQEFQINLLNQKIQNETQRAQHLESMLERQKQEFEEIQKEQMSSIEILQKQLESKQAVIQETSDHYNQVHELYLSLQARMEQKDQVNSELKRDMLQNKSKLESQTKEIQQLQQKIVSIENENLQSRNTMEIQQQGKLQDMTQKLQIKMRTIEQLTNEKTNLNDKIREIECESNKYKLTISQLEQQYQESQNQNNLLQTQIQFLNNKINDKEQEFEYLQQELNKQNQILDKSSEIWKKETDQMETDNNILKIQISDKQKEIYKIEQKLNLSLNELQQKEQQIDQLKLQLNNFNSNQQQQLELTSKDNLLLKKEIQQLNQHIESQHEEIDQMRVELDQKQLQVDENKSSVKKLSKQLELEIKQYSLHLKKNEDQHKKEVQELVDQLDIHQKQLQQKDMVIEQLNKQINGLQCAFETKEDLNHQTQKDIQQLKDKLKSDQNKFTKIIQGLENANIQLKNQLEESNHQIQENVHKYQSIQKQNDLQNIQFNEQQEKYNLLKDQFNRLESQMKQESQIREQYSHIADKVTKENNELKQKIDKKQQIIVQLESALLQNEETLKNVHDRAKEFCKQQEKEINDIKDQYLKLEAEHNAILSQNQEREDENEQMKDQKDQALRQMHTLEDENVKLKKELQEQHEKLTKSNNERSMLYKQIEEMQKDIAQLGGHNNPSQKIRYLNTIKQENSCLKQEKLYLSEQLQKEIEEKNKLLQQQQQNRKNTDSDNSQNTSKLDMQKLIVENQRLNNEMIKLNQILNLSGIKNRFSTIKGSDGDKIIQSVQLIIQELIDCKQEIQQRNKDQEGKNLKYQTMEKDLLVMKQKFYAVQKDNPQIQTKAQSPAVQHDVLRDNNRMNMNSGSVQRQSKPKTQQAFQFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V163</accession>
    <name>A0A8S1V163_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0520236</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000052">
        <property type="protein sequence ID" value="CAD8170263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V163"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8170263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="170" mass="20193" checksum="206A34FEEFF310C1" modified="2022-10-12" version="1">MFEQQKKKENVEQQSIKPISIFKIYQKEREYQVLLGLELLYGVVFLFQYGLYIKYYQIRDVKTSANFKQHDPFDDELADSIPNENDKEIMVYDFFRYSIIACLGLIINTIVKVYQYERSSMSYLKQTGIIVVYVIFVSNLIQIAEDKQIEHKSFLELSNKQTENDDPLQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V1C0</accession>
    <name>A0A8S1V1C0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0510142</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000051">
        <property type="protein sequence ID" value="CAD8169579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V1C0"/>
    <dbReference type="OrthoDB" id="2363873at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035727199" evidence="1">
        <location>
            <begin position="23"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8169579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="61" mass="7474" checksum="C88A336AB2F1939C" modified="2022-10-12" version="1">MDQRLLMMFIYWMLGFFNKVRVEDIEVDSPKLQTILFSDDQEFIYIIRNGQIMDMNQCIIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VCK9</accession>
    <name>A0A8S1VCK9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0570194</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VCK9"/>
    <dbReference type="OrthoDB" id="439844at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PRAJA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710:SF243">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PRAJA-2 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="115"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="203"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="266"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8172636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="305" mass="36423" checksum="91704E0157D6BC5D" modified="2022-10-12" version="1">MQNDAIDYLKIFTSLTIFKAFCSSVHRYKINQMPSSHYETESTLTTLMKIQMQFNFLLLHSTLFFKHLENVQEIGQFNLFLLISTCLFTMTLLLTDYRWLMKIYRYENHILREDYPIIEFLGIQIFMMFGCALLITIQWFTIPILSDLMYQLLSIYKIPQIIKCHQNNQMPNITYFCFLEDYCIFIFFSYYRGCPINIFKLQTNLGICISVAILICIQQCILLYQFYIKPQLYNNKSQVEDFIKQIREQNIYSTFDDLVINSNLECAICLQDPIVLTSCSHKFHESCLIVWFKVKKQCPVCRYQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VFY6</accession>
    <name>A0A8S1VFY6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0610280</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VFY6"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035905827" evidence="2">
        <location>
            <begin position="22"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="727"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="755"/>
            <end position="775"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8174779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="822" mass="95229" checksum="EFDD27B16D851A4C" modified="2022-10-12" version="1">MIQTFALFLQFLLCLSQWISSYTNLINDKNLTTFSQVGPSNSNYLVCNSPQIKYLTLNRDYPTATQKKQISLEKGNHISFELILQGTWQNGQIQFQFGAEIISFQYQTPNSFENFSIICDSYQSEIKTINFILQKSDSNIIKFTMSNEGNGQVSIRNLLISKIKCHYLCTQCNGIEYNQCTQCFYGTPLDGICNECPLNMVFRPGIGCSLFCNYQEYLDFNRHCQQYPSRQILFSDYYMVEEQVKMELILDTENTNHIPIYMSYVGRYDLLGVFRYNQGTKRLITEVTTNIGTYLIGIRTVIILYNEIPCGSSISLKINDTYAGSVYNDCQQLQFHKLRLVYIDILKLYQSYTNRQAYYLFLLLDIPQYQFTLSIVGNYIDLSATWAYENINLNSGYCPENCQLCELPNECKICYPTFYKYRDGQCILCTAPYQKLDGSYCKDYDDETLYSEYLIKEYYDLAIDPNQFSRYTLISKNGINFMKGSDILYSIYYGTYLIFGGELVWAQAKFSRVHQIIKPHHSITIGFYILFGPSFPQDSQFIYTIGSNNPVITFKGSSQTKRVYERIQHSSSSLTIQWECYGPNNEPKNAYCGFYNYYIAVHYCKPYCEKCTSQNDCTQWDNSYDPNIIKLSQADCDSNQYFDKNALNCFICPSTCLTCSSQFHCLTCQESFINSKQGCVCKNNQYQYSNYCYDCPIECANSQWCMNALKFITVFILMANAIVKMDIILLILIQFVISVQHIIVMFVIKLMEQNVILVNVVINQFKTIAFLFVVMEQKLQMNNVMMGIQQEEMDVLLVDMIAIIPVNFAIKESVQNVMMATF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VHT3</accession>
    <name>A0A8S1VHT3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0620127</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8175032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VHT3"/>
    <dbReference type="OrthoDB" id="541276at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="87"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="36"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="36"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="67"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="212"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="165"/>
            <end position="167"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="216"/>
            <end position="217"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="9">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8175032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="320" mass="36833" checksum="075D66A0C404FDFD" modified="2022-10-12" version="1">MKRQNSANVTGSPSRYFSTTQNQFTDILKQITQNCNNKPIKKPSSPNPNSQLQKRIKTQTSCSKIPNQNSQNKKASSSVPKNRLEYYTMQEQIGSGKFGKVYKCVLNSTKKVYAIKMIDKQQLKKNQMNHQLQREVTIQQMLKHPNIIQLIEFFETATNYCLVMEYANGGTLFQSLMRQNNKRYSEPAASNMIKQVALAIQQMQKQSIIHRDLKPENILWCDGVLKISDFGWSIQDKKERDTLCGTIDYLPPEMVYGQSYDNSIDLWSLGVLTFELTTEGFALQFPDHLSGDVKDLMKGLLTDKKNRKSIDWVLNHVWLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VK27</accession>
    <name>A0A8S1VK27_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SP-RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0660085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000066">
        <property type="protein sequence ID" value="CAD8176953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VK27"/>
    <dbReference type="OrthoDB" id="317074at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16452">
        <property type="entry name" value="SP-RING-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8176953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="884" mass="105335" checksum="96F88507BAC9B8BF" modified="2022-10-12" version="1">MLQFKNYESQIQDNLQKEKIIFDTIQLRNHEGNQYIKPVVTKKCIHSKGCFELDEIKKQLETNQLFTCAECSQNAQQYSDLMWDIRYYANQEFHDSVDEIHIIQGFMVNKYQRNKKYLDLLTKDDVKEHFVTLIQQNFETNSFLKSLIDKKQSGQQELYAICLLDFVKIIIPVRIQGCQHLECYEFTSLLYYQQNKKQSENSFRCTYPECNQRLDISSLNSFFYGIFIDQLLLKEIKQKNPVTIIFNINKQTKTFEQKIYFPLDQSLNSYSQKFNKQMTKQQELDISTNLDKVILQIIQTNLLLEKQDLKSSLLESTSELYIIDQFTKLPVELPTRCINCKDLNKSIDLLTYIFDFLYQKLMKNQQKYSCPLCNSPQNEIMVKIKLNQYIYIDQFLLLKLIKSKNDKYEQQNLNILSQNLQKSNNVGNQLINNQTLHSTENSQQISSTIINQSLKDMIYDRFSLLNTNQSKARYRNPALTKNCLHRSVCFEKEEALQYILQNKFYECPHCHQEAKSLADLVEDWRVQAYKEFNIIVEDVTIIKGIVVNKYIRNKKKYLDFFVIEEYARQFQDMFQRFTHDFSINSMIQKELVSQKSNNQTKINFWCFCLLDKVKINIPVRILGCKHYECYELTSLFFFQEQNRKQQEFLNCKQPGCSNRLKIAHIDYTKQPDTPKTSQKEEQERQDKINDIITLFSGISVDLDLLNAIKLSNPSSYKFYYNQDTGKIQEDINIENGKYVDPVIKAVYDQHPDFHFKITFEEFQKLIQQQAMSVSQGDLLQDDKQLGKKISLYKYRNYKVKMQDQLTKLTIEYPVRCLYGYEKLYCRFQLLKKSKSNKNLQLSIMQKTLKQADDSNLNLKLHIFRYKYAFIYVQRHVLHQWNKYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VKD1</accession>
    <name>A0A8S1VKD1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0670063</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000067">
        <property type="protein sequence ID" value="CAD8177181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VKD1"/>
    <dbReference type="OrthoDB" id="248233at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000264">
        <property type="entry name" value="GTP binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050055">
        <property type="entry name" value="EF-Tu_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721">
        <property type="entry name" value="ELONGATION FACTOR TU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721:SF9">
        <property type="entry name" value="GTP-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="2">
        <location>
            <begin position="242"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="637"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="665"/>
            <end position="678"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8177181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="761" mass="87627" checksum="2B178CC927EEC7D1" modified="2022-10-12" version="1">MHIDFQQQSTINNGIYSLWQGIDEEESKREKKKQQKQAHPQFSEMTDKQKQRKQNLNIQKKKKGKQSNKSDNIQINDDESEQQQQIQHLKYETKPQFILLVKPNNEEIQIDLQQYIDEQYSIQENISKISCFKQESDFGHTEYKLRLVDPDPKRLTHLTTQMNFRLSEGNGMAYYKIGVEDSGNPLGLNKSDMLGSLKTLCLMAQSLKAELLVVGINQGTNGKTCEVIVRKGLKDGINLDIRILLLGESGSGKTSLLGVLSTGQLDNGQGLARMKFLKNRSEITSGKTERVSHTVIGFDSEGKIMNHQNQLNFSPTCMVEKLVDLSTKLITFIDMGGTKRAHNQMIQIINSQFPDYALLTISSLQQIGKPTMDFLKLIQIQQIPFMIAITHVDQITEDYYMDKVEQLKDMIKSLQIQSVPIVVRSEEDVVLFSKQILSKTLIPIFLISNLKQRTLDYFIKFLNLLPSTNELTNNSNLDSEYCIHNVFEINNQKVLGGTVLKGIIRVKQILQMGPDKYGRFFPIEVEEIQCNRVQVKSAQCGQICTIRFKQGNLTQQFGSDQNPIRRGMVLIEGKSNPQAAWDFLAEIWLFDDQKEAKKIAVSFEPVINTQCTRQICKIIREEQLQFENLTLRKKQSKSIDESCMQKPETQQQRTSYRKQSEQLKKASSSNNLNKQSYRSPRRKGSSQIKSYTNEDDQKKTDLFVINEKNEKPMEFIELIPKQPNIIRLKFKYFPEYITKGMKLIINDNGLQAWGFIKYINY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VMA7</accession>
    <name>A0A8S1VMA7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0690115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VMA7"/>
    <dbReference type="OrthoDB" id="311561at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="280"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8178310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="299" mass="34798" checksum="5CF23F75B56E4F05" modified="2022-10-12" version="1">MKFYHSQLSSKSFIAYHLLSITTGIFFILPLVFTQMQFIVCTITMGIFGICQCIIFSRFQKLQIYIYSFIISLSLLLSNTYTKALIIGTCLGLIINLQNQFTQILQFQFLQECQYFGLLLYYLLIKNISIQSISIFTFISSSIIRLFLCDYNIDLTNSKPQPLIIKQKQQYLSILGQFVIDLIFFGTLYLLYQFQNHLIIIALTLLLFASIIVNLFQNYLKQLTYPILIIQIIVIIQLIFIDVFILIPFDIIIIILFVCRPINDIYLKNNLGFEMYQQEHSNSLIISTISTIFAALLIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VN05</accession>
    <name>A0A8S1VN05_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0680235</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000068">
        <property type="protein sequence ID" value="CAD8178041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VN05"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8178041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="50" mass="5857" checksum="96CBF1E723652C1D" modified="2022-10-12" version="1">MMIEQCYVETIFGNIFLKPLFLKSQITRFDLTIKLKQNLNTNSWSKGCLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VSK9</accession>
    <name>A0A8S1VSK9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0740007</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000074">
        <property type="protein sequence ID" value="CAD8180240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VSK9"/>
    <dbReference type="OrthoDB" id="10249433at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF81">
        <property type="entry name" value="PROTEIN ABHD13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8180240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="301" mass="34130" checksum="BD60DCB7B7A7CA58" modified="2022-10-12" version="1">MEFIQSLLSLFGNIAIVALMLTIGILGLLYFQQNSLIYLPAFQGLPQSPSQNMNGLRNPSERNLQYEDVEIGTLDRQKLKGWLIKQNDSLNVPTVIFFHENAGNIGTRLQFLELYFANVKCNILIIAYRGYSDSTGKPSEQGLQLDGESIVNYLFHRNDIDPATIFVHGKSLGGAVAIHAMTQNIAKGIRGVILENTFTSIDDMVDVIFPKLKLFKTFLLKNHWLSIQKIGQITQPILFIYSMRDEIVPVQHMAQLQNTAQRAKFIEKFVIQDGDHNMNWSREPEKYFNSISSFINKAITS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VVS2</accession>
    <name>A0A8S1VVS2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0750010</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000075">
        <property type="protein sequence ID" value="CAD8180747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VVS2"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18045">
        <property type="entry name" value="DEADc_EIF4AIII_DDX48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000849">
        <property type="entry name" value="ATP-dependent RNA helicase DBP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000031">
        <property type="entry name" value="Eukaryotic initiation factor 4A-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="12">
        <location>
            <begin position="22"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="53"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="234"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="8">
        <location>
            <begin position="22"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8180747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="395" mass="45404" checksum="7DB0050FBE3AE56A" modified="2022-10-12" version="1">MDKHNLQDNVEYETSEEITIYPTFESMQLREELIRGIYAFGFNKPSAVQQRAIVPIIQGRDVIVQSQSGTGKTAVFSLSALSIIDLSIREPQVLILSNTRELAEQSSKVAMALGDFLNVSIHACIGGHSIQDDISKLQHGVQIVSGTPGRVFDMIQRKELRVRHLKMLILDEADEMLTKGFKQQVYDIYRYLPPTTQNVVVSATLPQEILDMTDKFMNNPLKILVKRDELTLEGIKQFFIQVDKEEWKFDTLCDLYDTLTITQAVIFCSSRDKCEWLANKMRENEFTVVQMHGKMSQKERDKIMAEFRQGNKRVLIATDVWGRGLDVQQVSLVINYDLPISRELYIHRIGRSGRFGRKGVAINFVKQEDVRILRDIEQYYSTQIDEMPMNVTDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VWF0</accession>
    <name>A0A8S1VWF0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0750222</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000075">
        <property type="protein sequence ID" value="CAD8181141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VWF0"/>
    <dbReference type="OrthoDB" id="10043504at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="170"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="62"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8181141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="390" mass="45903" checksum="BD31837B411AEED2" modified="2022-10-12" version="1">MRQQLNTKLQCQQQCHQEDVDMVCYNKFCTELRLNCFNCIKKGSHKSHIDDVQKINTIVAFIENQNKQCDNLIDNLNTLMKSVNQSFSQLKNGIKNKYSLLKERLENLNSQQVNDFLKSTFEFPEYKQSIIHIIQEQSNQLTQTFGSLHQQLQLLSINYDKIDDKDIKLSKELYDQGFQLYLDDKYREAIQILKKSLQSDPNNNLSLWCQGECLSRLDKYESAINQLDQELALDPKMLIYYGVKVFVYQDKEKMIKLSFGQTKLQPLILSILNPYALKANAQDQCSNTKNLQDYLIKHSPLTDSMLHHLTQKENVQQIFKITKKLREIVKKQYIYKKIMYAQMQKGLFQTIQNDKINEFKQLVSNLIDNFIIIFINFNSIRIIGNLLIYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W3S5</accession>
    <name>A0A8S1W3S5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA repair nuclease/redox regulator APEX1</fullName>
            <shortName evidence="8">APEN</shortName>
            <shortName evidence="8">REF-1</shortName>
            <ecNumber evidence="8">3.1.11.2</ecNumber>
            <ecNumber evidence="8">3.1.21.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">APEX nuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Apurinic-apyrimidinic endonuclease 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Redox factor-1</fullName>
        </alternativeName>
        <component>
            <recommendedName>
                <fullName evidence="8">DNA repair nuclease/redox regulator APEX1, mitochondrial</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0830161</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="6 8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="6 8">Probably binds two magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.2" evidence="8"/>
    <dbReference type="EC" id="3.1.21.-" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDO010000083">
        <property type="protein sequence ID" value="CAD8184634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W3S5"/>
    <dbReference type="OrthoDB" id="498125at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09087">
        <property type="entry name" value="Ape1-like_AP-endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000130">
        <property type="entry name" value="DNA-(apurinic or apyrimidinic site) lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020848">
        <property type="entry name" value="AP_endonuclease_F1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00195">
        <property type="entry name" value="exoDNase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00633">
        <property type="entry name" value="xth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF6">
        <property type="entry name" value="DNA-(APURINIC OR APYRIMIDINIC SITE) ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00727">
        <property type="entry name" value="AP_NUCLEASE_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51435">
        <property type="entry name" value="AP_NUCLEASE_F1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 6 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="10">
        <location>
            <begin position="90"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="13"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="5">
        <location>
            <position position="239"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="7">
        <location>
            <position position="310"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA substrate" evidence="7">
        <location>
            <position position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8184634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="348" mass="40179" checksum="7FFA16E40E25525F" modified="2022-10-12" version="1">MNQKRGRQAIKRLSPDKKDEVQQKDVVKSKGKQQKEVKKTEDTVEKKGEQKIEKQQIIKGEDKKVKQVLEIEQKFELGDGKFSTPTIKIASWNVNGIRAATKREQAVQYLHDNKFDVICLNELKADQAVFDKENLGSKFGKHYVLYLNFCKTKGGYSGVAIASKVKPISIKCDIGIDKHDKEGRTLTAEFEKFYLVACYVPNAGQKLERLDYRTKEWDVDFQNYLEDLKKKKPTILCGDLNVSHHEIDLANPAGNKKTAGFTQQERDQFTNYLQKGWIDTFRHLHPKEVKYSYFSARNNSRQENKGWRLDYFIINKEVTNAIIVSDINTNIEGSDHVPIECEVDLRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W6H6</accession>
    <name>A0A8S1W6H6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0810029</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000081">
        <property type="protein sequence ID" value="CAD8183499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W6H6"/>
    <dbReference type="OrthoDB" id="303047at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8183499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="23407" checksum="40D5CB99E93DC459" modified="2022-10-12" version="1">MELENNKIEEITYLIRQKQIQLEFDNSDKNLHDIEQGIEEQDQRIKKLIEDLDYGDRLEWIEYHKSQGSLYYQKQQYRKALNEYYLSMLALNNSKMWREQGISLIHNIQLILEYLKIPATKELLEFVLYIDIYNIKSYFKMGKFYSGDKKYQIALSYFQQGEKLCNQMQDKESQQDFQKQILDCKKQLNLGRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W6W0</accession>
    <name>A0A8S1W6W0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0830202</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000083">
        <property type="protein sequence ID" value="CAD8184703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="309435at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18921">
        <property type="entry name" value="MYOSIN HEAVY CHAIN - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18921:SF2">
        <property type="entry name" value="THYROID RECEPTOR-INTERACTING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="661"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1017"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="237"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="327"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="571"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="931"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="661"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1017"/>
            <end position="1035"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8184703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1367" mass="164523" checksum="F1D95B244DB3B801" modified="2022-10-12" version="1">MSLSNQIFSLLRTLETYKQIKQYIIRLRDQDENVYQPICDAIIQGLNVKDHVQNLMAARLGKDSVEQQNMDFNIIFQEVCLPEYEKIAEFKKDDLSDDRGRYYFSETPNKEQSSYGISLLRIILESIEVWAKTYIFKSDQITQTLFFETYCKLCVKKIKFPHEYKYYKIDEIQKNISSQFQILFQNLIKNQNENKSNRKQQTSQIIQQDQQQKQIKVKKPILIRKSSSISTPIDQKEAEIISKAQELKNTFNQLKNQLSKKALRKEFKSTQQYIKDLTEVNQSLEQLMKDLDNKFDEFLRQCDEDIILELMEISDYLKVLCQLFEKLQKNELTLQNYRNKVEKYTKQVPEQQKDKLNYEQQMEDFQIQYNEIEQKEKEKQIIQQEHEKKVSNLEQQITESNQQRNLLEGQLKQLQINYRELKNNSIQQQDLERMNNNLIEQIRQLNLQLQRQSEENSLKIENKRYKISKLKEDKQKIQQEIENLQSQYNSDITQLINQNRELTENYNRTINQFQDQNQLNREISELKQQLKTERKKSGLLMDENKQINQTILSLQQQLSTRDQTQQTNQYVGQHNQDLQQCQAELINYKQQLRQLEALKKKEENQYKEKINNLEQQLQIQLKESQKHQIQSISLNQQNIELHRELQKQKKQSGQEQIIQFENQIPKSNNNLDQATQRNNSNKREQTQTSIPYFDYQHFQNKQIDFVENVQNLFTKTQQQKIPVKSEIIIFPYKTAESYYKKKVQPHKGIVVKFHNFLQNQLINQNNLTLYKIYHLNRINHTIFVDNQDLKIGVIKQVQQNSFNYYINYGLYLETGFQCLKLKACIKNLMNFKTIWTSFPEIQQNIYYKQQMILEVSIKVRETEMNEVPILEINYNNKIEQILLPIQILCLIQYHQINQNRYQTRWKTSKIYRSEVFEYNQLVLTDHKDIXNQDLQQCQAELINYKQQLRQLEALKKKEENQYKEKINNLEQQLQIQLKESQKHQIQSISLNQQNIELHRELQKQKKQSGQEQIIQFENQIPKSNNNLDQATQRNNSNKREQTQTSIPYFDYQHFQNKQIDFVENVQNLFTKTQQQKIPVKSEIIIFPYKTAESYYKKKVQPHKGIVVKFHNFLQNQLINQNNLTLYKIYHLNRINHTIFVDNQDLKIGVIKQVQQNSFNYYINYGLYLETGFQCLKLKACIKNLMNFKTIWTSFPEIQQNIYYKQQMILEVSIKVRETEMNEVPILEINYNNKIEQILLPIQILCLIQYHQINQNRYQTRWKTSKIYRSEVFEYNQLVLTDHKDITKLNQCFIIKNRDKINSYNQGLDEIKYIAQFGLKQYNVEGIIKFELMPDYKMIIYVGIENSMNNNQLIQRLINAYQNIFIQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WAU9</accession>
    <name>A0A8S1WAU9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0870112</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000087">
        <property type="protein sequence ID" value="CAD8186511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WAU9"/>
    <dbReference type="OrthoDB" id="21530at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="86"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8186511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="172" mass="20649" checksum="9A67641EE491E317" modified="2022-10-12" version="1">MLRQKEQIEFLSKKPPTCIKSTIETLMMNFQQISRNIDHLDQDLNQLSKRLDYLEESMTEHCILIQPQKQETKKKRRTANLIKRDFQCPYEKCDKIYGTDISLNLHIRLKHNGGSKIEREELAKKILDAKFQDEIEPEHSFNLPPNFIEDFQIHHQEYIHNLEKTYNKRILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WBM3</accession>
    <name>A0A8S1WBM3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0850035</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WBM3"/>
    <dbReference type="OrthoDB" id="74764at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="138"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8185339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="523" mass="61186" checksum="654E1BE34096B729" modified="2022-10-12" version="1">MQQNYVWRKINYDIKEKPILQFKFQKVGKKSKDIYIGCFNEYLIKYKDQFYTIPYKLFDLVFTSKFEVIRTPSKLKQEKNHSKVRGEEILELGEIVAIRLIVENIDKPYEIQGKTDQMHQLRNFLGKKINQIGFHNLFRVYKKIGRGNFASVYLAERIEDNLRMAVKAFSKQIVYNEEKGREGLINEIQIMRELDHPNLMRLYEVYETMNSIYMGLELLEGDQLFEYLKKKISFTEKQVQSIMVGLLLGLKHMHSKGIMHRDLKLENILFKQHGNFDSVVIADFGLATHVNKKPYLYQKCGTPGFVAPEIINLKNLYQPYDSVCDVYSMGVVFYILLTGRPAFTGKTYNTIVKQNKEANVNFDSPHFQNAPKEAQQLLFQMLQIDPQQRIKSSEALLHPYLQSVLLIDDDEAHISTDDDPNLMERIKILNKQTEKVDFTRIKRQNSQNIKELRLQATQELKQNQTSDEELKIIMRTPVITGRINSVENSPMLKYLSPFQSTGYDEIAQDQLILQRYSGKTLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WE46</accession>
    <name>A0A8S1WE46_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PWWP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0820172</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000082">
        <property type="protein sequence ID" value="CAD8184166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WE46"/>
    <dbReference type="OrthoDB" id="62853at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05162">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PWWP" evidence="1">
        <location>
            <begin position="11"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8184166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="237" mass="27502" checksum="8CE4AA30F3E22864" modified="2022-10-12" version="1">MQQGCDVKFEINEVVWAKVLGYPWWPAQISSITNGKTGSNQLCRVNFLADGTHADLRLEKVKKWNDRPIQTETKKLKEAIELANQILTGQYKNILPSFENLNEHILKSLVIKLNDLMRKDNQIEDIVQSLNEISTNELLSIVKMNLGQQLFQLHQIYQNRKQKEFQNLLHSFLDQLVNIIGYKEQVEQTTSNKKKFKQSISNEEPLEEITKCVIIQDGPDVKRKKVEENLQNTSLNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WEG5</accession>
    <name>A0A8S1WEG5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0870131</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000087">
        <property type="protein sequence ID" value="CAD8186549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WEG5"/>
    <dbReference type="OrthoDB" id="300629at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8186549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="278" mass="33002" checksum="E122CC3F7EBE3C70" modified="2022-10-12" version="1">MDQQIAFYLKMISYYEQKLQTIKQTLYSNNIDNIFRSFELLSNSEDTITKNNLLNFFVQNLQPINYKDIIEFIWFIGDKDRFTISKQQFHNYLTKLVLHKSNTIIDHTNNDLQVQLLKLLIQQIRVIKQIKDSKLQIINNDDFNLFKIFQNLSDNNDIINCTSLMSYMKNNKVQFDGFDFDLLTFAIFGKKCNISFDQFKDCSLFSINISNEQYIKSREIPEEKQMTHSYIINLSKSIIQQQQLMPISEASSIVEFKASELKPKTKRYQEQINVPRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WF02</accession>
    <name>A0A8S1WF02_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0900081</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8187863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="377346at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="1"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="8">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8187863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="180" mass="21008" checksum="8321EC6414F16475" modified="2022-10-12" version="1">MPKYKNFKTIKKLGVVKYSEVFLTLQIQTGFLVALKVIQKALIIKEMMQSQLESEIVLVLEYCSHGQLNTVQQTKQNKRFTEKEAAQLVQQITFALMYIHNLDLIHIDIKPDNILLNFGQVKLADFSFYVYSPDEYKQTQCGTLIYASPEILEDDMYDKKIDIWGXNNNLYQTVIKQIFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WF84</accession>
    <name>A0A8S1WF84_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0890016</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000089">
        <property type="protein sequence ID" value="CAD8187327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WF84"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8187327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="210" mass="24482" checksum="FA7C9F859C7FC204" modified="2022-10-12" version="1">MIVGQLISSQQYRITRCYFIQLKSEEKCIKMSQQYFNEIVQKSLLNFKNNYPPIPSYATYYDSFSKTDCRKAAHCTYCWNYSACAQVENKFTFFQSCGAFAKKSDQKCQAISNRFKSDGTLLMEIDDCTTYKKQIEFMLLEFNKQYMCRSNTCDKLPQSLISEKVCRDAIMTCTTKSEDGCIITKDNVFGINQKQQFISGIKQKLQRQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WKX1</accession>
    <name>A0A8S1WKX1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0920097</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000092">
        <property type="protein sequence ID" value="CAD8188799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WKX1"/>
    <dbReference type="OrthoDB" id="304129at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00075">
        <property type="entry name" value="HATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="456"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="701"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="757"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8188799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="829" mass="96841" checksum="922B13EBBB33EEFB" modified="2022-10-12" version="1">MLIYTSSIYLQFSIKNTIFLVEVGMQQQTQLQYIIINSFSLLIAVLLFGMELFKKQPIYINRVGALILCLLNVELVYQEISQINTIQLVIFIVILITQYEDEERWHKIAKHSIIFYIFLRTLLQYKDLFNITQLLTYVLWQPINHWMLHYKNLLKINQLKKQSLDLSKCQIELTPQLCKDSSEQEELCSSNFQRSSKLSQNIKQLKQLYMNNPFSQMADLDQKTFSQFQKQTSTLDVPQIWNLLPFGIALINSKFEILTYNQKLLYFLKVSDNDGRNIILNLDLLLESPESWESKSVNQFSSHCSNRQSRRFQTKRQLSIISKSNHEQNPDGGNNSQINANFAFIPNTNNQIRDEILTTKSRYRNLDQLLKKFALKSYSLTNNGDCSFQSIGQNIQIIKKVQDIDSKKYHFRIKVYELDTIGNKINYLFVIENITNKEELRQLSVRYKFQQVLLNSLCHELRTPMNSTLSQLNALTSLISPQIRDKHLIPAIISAKKLMFQLNDILDFAQIDCKNFHLVNSQFELNEIFEILRELFEQECKEKNLRFVLNYQNIKDNQNIYSDKERIVRILVNLIDNSIKFTNEGGIITVTVQQENQYILFCVLDDGVGISDKTLTSIRSNKDFQMQDQYNQNETKLGLGLKISQQIAKFLCVDKELKFESKEKQFTKISFKIESNSKQLKHLKLPSFQGCNFNINCDCIQILNVDDVRFNHSAMEALFCQHKIKMESAYNGEQAIQMIKRRLDQNCCKTYKLIFMDIEMPIINGYQASKEITAILTKFNLTDQTVIVMCSAYDGYENSNQAKACGIKEVLPKPIEQKQLKQLLDKYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WL16</accession>
    <name>A0A8S1WL16_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Potassium channel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0920122</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000092">
        <property type="protein sequence ID" value="CAD8188849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WL16"/>
    <dbReference type="OrthoDB" id="415460at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="345"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="389"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="421"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="3">
        <location>
            <begin position="369"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="488"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8188849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1030" mass="122016" checksum="B4EAC09ACE28D850" modified="2022-10-12" version="1">MFNIEFDQDVELRAVQNEDFEQQYLDRCLREYRKEPNLTIPQISRTQSQKTIEAECLHKISETPFDENVILTQAQNSAHFSNKYLQKHEKRFSIPNAGTSLMCSFYIRRFIEKISQSRKKLLSMNEIHFNLIEDKAADMQNLFNHQSECKRQGYTLGKMKTLLRKESVINDLSTQEQIKEQIRKIKKQILNLLTVIINKIPIIQPESRLKMIWDFFASFFRILLVILIPLEIAFQPCILFTDFIVLTAMILLILQIDFLIRINTLSYRNGAAIQNRWELLVYQFKKEFFTDFSTSFLLIIFIMIPEIDNNLNLFLLLTLAQYKYIHETFAKSDQISYLTRPLRGIIGLVKFILTLLFILHLFSCIWFWFSKISLEDSWIKFNGLDLKSWELQYLEALYFAVVTMLTIGYGDNVPKNSIEKIITIIFILGACLWFSYSVNFIGGIINDITQNQVERNRKMRVINKYMDQRKIPFNLKHKVKEYLTFRWKEDDEVDLEIEQTLLEQLSDELKEELDKEAHKIFIQKSALLQHFSDELKDALSKSIKRKIIPPQNTFSIDFDDHQHLCFVEQGVLLYQHLDRKQRSKMNAPIKQGQFFCVQDFIIQSHQKDYFKSNGYVSLLILSKLDFMTTIKNYPEDFQKFYEMRELMTLSLKPPQLENGVFCPACLCFNHSLAKCPQIQYIPDREAILKRYLLPRIQERKQYNRDLTKRFNSDQVISRSRLEKDIIQQFAQVFQSENHQLIQEQQKVQLIYEQESLSSCVDPSPCNKFQNDGGFQAAVLKSKRLISETSIQLSSNAKLQDTKSSLHQALQSRKQSIINLNIPSNIGKSPRNLLIPKVAIIKENEENEESSNCDSDDSKSNRNQEIKYQLSISDFNENMMENVINLYHQLQRELESNENDQLIQKAYYQIEPIYWSFHQQNLSEFEMMTTYDYFFKSYNYNEIIRLAQKNTFSWQSQFINKLQRYMLYPFLFIQKYMSKKRNLNNTEKIKRDPSKRLISRLNTLKNSLKLKKKQSIIIKPTQKFGQVAPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WRR5</accession>
    <name>A0A8S1WRR5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0990060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000099">
        <property type="protein sequence ID" value="CAD8191480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WRR5"/>
    <dbReference type="OrthoDB" id="312479at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051425">
        <property type="entry name" value="Formin_Homology"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725:SF1">
        <property type="entry name" value="DISHEVELLED ASSOCIATED ACTIVATOR OF MORPHOGENESIS, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725">
        <property type="entry name" value="FORMIN HOMOLOGY 2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="317"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="347"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="363"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="379"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="401"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8191480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="960" mass="112705" checksum="90EEFDA2C491D982" modified="2022-10-12" version="1">MFGKIIQAEIIKTNQKVQFKISQFTNNEEIDQKIIKNFIKEVDIDLVHITNLPILTQLTVEYNRMMGNNQTYSIENVQNWIKSSELIELTYALRWVYEKSSEKEREEIDIYFDTNDEILKKIVLLNICLEQKKEISISNLIELVSDNTEILYGLSLYLQKIIETQQDFQKYVPLLLQMIQQGDKTAINQLIIVELIEKVLTKFSKQKNELFDELIFNDYIIQQLEWQEDYKNYTLKELENPNLSLRKTKQAYQYTKDQYEIIIQKFKSQWEEKLAKIIKSNQSEQGNQITMSMDDMFSSAVLEESQQEKQLSAVLEKSQQEEQISTLRRRKTKVIRPINPESIPNPITNPPPAQSSVPPSPLIQGQSGISPQSKGQSGIPPPPLSGVPPPPPPQSKGQSDVPPPPPSPPPPPPPPQPKPQSGTKPIPPPPQKVQQSSAPKVAQKAPGHPDYIKFNYPIVENDIEGTIWKAQLQNYQVKINEGILKQFEKKQKQQQTEQKKVIQKLIQKEVVEYLDSVFGNQMGIQLKQKILQKIDLDNILEQINQLEFLNQNKDKEMRLNIDHLNEIIEKVTQDNIIKIQDNIIKAKKSSQAYVKKRLEEEDQLQKQEKERLTYLSIEDRVLALFELDSNVNSCQNDLQTTLGEYEVQKILSQLEPELEQYRKTLITTESEEQKNDIMLQFEEALFKKQKLLKSKKLQIQDKFQVPNIDLFLKYLYERNTRKGIQIEAFYKEHLDRKIHIENAVTQYSEVFEELKKDEELILYFQYIKEYGKVFNNIKADQFGFKFENIIAFSYKTQQLEGSQEELIKYIVEHMIKQGIPFTELQNTPFKHLQTLSQEFNGLNEIQNLIKQYKTHQRFLETNLQSLSQDEENTEFIKKAKKYVSEYKDFIYQMETIYQNDQNNYQQIRNFFCDDRPNIESPIFFGNIFQIKSLLRNYYNQIQSEIKKKKLDLARSRRIGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WS31</accession>
    <name>A0A8S1WS31_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0990130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000099">
        <property type="protein sequence ID" value="CAD8191620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WS31"/>
    <dbReference type="OrthoDB" id="286864at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021258">
        <property type="entry name" value="Epiplasmin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10992">
        <property type="entry name" value="Epiplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="390"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="390"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="413"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="433"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8191620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="445" mass="51190" checksum="039D1151A0576484" modified="2022-10-12" version="1">MANIPQQQYSTPSNLPSQNRDQSTYQQPTLGQQQVPQSIYQPQQSGEKGLQYLPQQTGILPQNVQTQQLYGQPQVGIIGQQFTQQPIFGQSQIQQLPQQTVETQQGQVVKGQSRIEYIPYERTITEYEEVRRQVQVPITKQVTDYYAVQYDIEYIPQVIQEKQIEYVPVERVAERTEYYTVERQNVVQQPIGYQSSQVQTSATYAPVQTQQVTQQYTQYVQPQQQTIAYQQPLQIQQQYQTREVIQQPIVQQQSYLQQREILPQQQVQVQQSVALQPTIPTTQYLPQQQYQTIQTTQPQLQIAQTVPLNYGQQIQTIQTQQQPIVTSPAQATTQINQGYSIPVTQTQAPQYSVYGQQSLGPQQPKAQQSLTQTTQYNPQLQQTVAPAQFVQSVPQQQQQQSIPQDMGRTRPYQQGQLPQQPSQPQQSGTAQKQNKEKSFLEKLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WSM3</accession>
    <name>A0A8S1WSM3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">A-kinase anchor protein 7-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1020203</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000102">
        <property type="protein sequence ID" value="CAD8192858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WSM3"/>
    <dbReference type="OrthoDB" id="277832at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006307">
        <property type="term" value="P:DNA alkylation repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019510">
        <property type="entry name" value="AKAP7-like_phosphoesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009210">
        <property type="entry name" value="ASCC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13360">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13360:SF1">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10469">
        <property type="entry name" value="AKAP7_NLS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="A-kinase anchor protein 7-like phosphoesterase" evidence="1">
        <location>
            <begin position="14"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8192858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="231" mass="27726" checksum="758CADCBE1CF4165" modified="2022-10-12" version="1">MKQRRFVQKNQATFTHFVSVPLILQPTQGKLQKLMDDIHKKYNFDQSMNPNNPFLFHITISMLGLSNQEKINKARQIFIDNEQTIKNYLKNSSIKLKGLGCFQNRLNQQNKYYKRGPYEDLNIIYLNVDETPLLPVSDFIIRQFLQAEIFDSDDLKSMNLIMDQQSKMFRAEKFHITLFRLKDCKINFQQLFDEYKDYQFGDVPIQYFDISTRWQYDKDKFYQPLARIVID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WT35</accession>
    <name>A0A8S1WT35_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1000079</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000100">
        <property type="protein sequence ID" value="CAD8191911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WT35"/>
    <dbReference type="OrthoDB" id="292516at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8191911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="447" mass="52245" checksum="C25E8C04D71432A3" modified="2022-10-12" version="1">MMKVISRMSSLILKKSDDMILFAHDNQVKQGEVLKFGKSLGVVMSIFEKNQLALLLQQSEVLEPIQHIKSLKAYPQNNICTIADIKESNENNTDSKQLPKRVLPQNQILTHNVYIDLFHPITYGQMLIIKDKELLKKLNAFDQIIYYGRSPIQTATWFKHAESQGSNYFLPKYALEYANQIAKSNKTLLVMDQIYDHFVAHQKLQETIQSYLYEKTTKTVYLSNLLKDLSQSCGYRPGHSNLTVAVLQRDSRDDLLNDELEKELDSLQSHTPSATLAKPSSLSLQIQSPLYYHLAQELYDILSQYNQKKSEYMQKQQLKIHIDPWDFYEYFDCKPIVQMINLFNQQNSQEHELAILVDFSVKAFKQELICQLKGSPKEVLNDLLAFSCSIKEFDKKTFAEFYYEKKNELDGNQFKSLIIENLGRFYELYMIHLRMTDQLLQYKKDYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WVM9</accession>
    <name>A0A8S1WVM9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Malectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T1060026</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the malectin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WVM9"/>
    <dbReference type="OrthoDB" id="663146at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021720">
        <property type="entry name" value="Malectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039155">
        <property type="entry name" value="MLEC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13460:SF0">
        <property type="entry name" value="MALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11721">
        <property type="entry name" value="Malectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035918349" description="Malectin domain-containing protein" evidence="10">
        <location>
            <begin position="17"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Malectin" evidence="11">
        <location>
            <begin position="21"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF11721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8194104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="377" mass="44026" checksum="090AF2DCB0F00C52" modified="2022-10-12" version="1">MLLFLLFIYVNSYLDANKIKYAVDAGNQYYYKDRQKILYRYDQNYKGGQQLMFYQKVTSDQNHHLIPLQYLDYYVFQTSRTVSSDQDFFKYSITMRLNGNYTLILCFIESEQRSARFFDIYLEDELIKEQFDIYKEAGGINIPLYFAFEFEFDGKNITTNRKTIYQMNDNKLHFDLTFKGKGASVSAIILYQGKIEDILIDLESALLSKDQIEATFINQIEFVKALKQKAARYAHKQKGKGNENVNTLMNQYLSPFQKLINNFDIGNNVYNEISNGLDPSPLELFLDTLLNNILMPLGSVLVQIIDFILDNKVLSTILMINLILYYYCFDNNKIMFANVPVGLVLDPEVESEIEINYNTQSPKSRHVNVLKSKKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WVT8</accession>
    <name>A0A8S1WVT8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1010056</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000101">
        <property type="protein sequence ID" value="CAD8192269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WVT8"/>
    <dbReference type="OrthoDB" id="194358at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24126">
        <property type="entry name" value="ANKYRIN REPEAT, PH AND SEC7 DOMAIN CONTAINING PROTEIN SECG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="278"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="311"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8192269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="383" mass="45385" checksum="9A8D1332BC8ADC1B" modified="2022-10-12" version="1">MFEQEYYKRIIDKQITPNKCTINTGKPWFPQLKHRLALKDYDYKFFVLPPNKMVSLLKKQKINVPSDEQIREFLRNHSLKHLVQYDALDFKNVDKICSSNSPSPLMINKKNRKIFMQQNPNTNYKLIESKPQTAQKLFDRQSSVQHCRMRELKSQQIEIRPNRENMELEVKGIKKKMRIHEISTKSTPQIDASNLKYCTQSNKIRIKRKIDLLSRSLKKINLLGLSIIDVIQKNIFSKKAFSCQFSKEFIFASKQNRLEEMQSFLITHPYLVFQYDYYNMTALHWACKYGYLDMVRMLLDYHADFDALDLMNRTPLSIAIIENHQEIVKLLLTHGAYPWSTSLTDLNGPLQQNQEMKKIVGQARKIQLFTKWSRIKQTTLCIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X4E9</accession>
    <name>A0A8S1X4E9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1030139</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000103">
        <property type="protein sequence ID" value="CAD8193156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="410989at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332:SF175">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="25"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035824341" description="EGF-like domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="1303"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="34"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="67"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="98"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="176"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="210"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="252"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="301"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="420"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="466"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="522"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="562"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="615"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="659"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="703"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="747"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="798"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="844"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="883"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="928"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="958"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="989"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1067"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1109"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1218"/>
            <end position="1251"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1259"/>
            <end position="1299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8193156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1303" mass="144612" checksum="A2031608FA9AB241" modified="2022-10-12" version="1">MNQNTLLILLTVSSIFQGGFGCPAGTWGISPCLPCMSQCATCSIATQCNSCKPGYYYVSSGFGQCPSCSSVCKTCTSSSTQCTGCNDGYYLSGTNCPACQSPCSLCITTNSNCTGCVAGYYISGSTCVTCSKPCAQCTTSPSNCTVCIDPSNQAIPSCDCTQPNKYLDTITYMCNNCVSPCLNCSSATICDSCVNGYVLSGHTCIPCTKPCANCITNQTTCQTCVDSVNQIPSTCNCNSGFIMNLSTFMCDQCNHPCATCQNTIIECHSCASTYQYDSSAHTCTCLTNQYEDNGSPKACQNCSSPCLTCSSNTDCNSCIIGLNRHLVVNSCICDDGYYDNVGVCTLCSLPCTKCTSPTFCTECNDVSHQVLNDCHCIDTYYMDASSVCQQCISPCLNCSSNSVCTSCINNYYLDSPNCLQCSLPCYNCVDIDTKCTSCAHSHQTVQSDLCVCDDGYYMDGSYYCQLCTHPCSKCTSTSTYCTDCASTFISSGSNTCVCDDGYYEEILNSPSDCQPCTSPCVKCEIESTHCITCIDINQTVDPSTYQCLCNIGWVANGNLCDQCVSPCTQCSGTTTYCTGCKDAHHVLSSGQCICESGWLNDSNYDCQPCISPCNSCSINTSHCDSCVDIHHIINGSYQCICQDTYYSDTISHCEQCVQPCANCDLNGCLTCIDTNQYIDTNLDCVCNNGYYMDTVNCLLCQLPCVNCTSSLDCLTCIDTNQTVVNHRCICNHGYYATGNYCNQCQLPCTKCITTQDTCTECLDPNHVIINNKCICKSGYGQSGLNSNYCSMCEYPCLECSINVNTCTKCIDSGLFKVESNKCICQEGYFKEDNQCIRCAPQCKACEQFQDNCLICKDATFIQFNNQCFCDYGYFINQSFQCKPCQEPCTTCQYNNNFCTSCIKDQQYLINHTCVCQDGYYMQNYECQKCNYTCTKCFSFNICNECIDGYYLYNDICEQCDNQCLTCIDHDVCLSCSDGYYMAQMYKCTSCISNCKECNNAYTCITCFDGYFINDEICLKCDDNCNTCVDEYQKCQSCNSNFLLIDNKCICQEGYYYQNYECLKCQYPCKKCENQFKCNECISLTNILLNDQNMCICQDGYFWLENQCKECDETCQTCYLQSQNCLSCKPESNRVLIKNKCQCESNYFLNELNLCQNCKSEEGKIIKSCKYKNCADSQWTYGEECDDGNDTNKDGCSNCIIDKNYSCINTLLEQSICFQCPQYCELCDKDNINQLVCKKCQSGYFLDSNSCNKCNEKCKECVNSSSNCLICRYHQDDQMQCKLCESMDGYYTDYKNNSCQRRRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X9F9</accession>
    <name>A0A8S1X9F9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1160034</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000116">
        <property type="protein sequence ID" value="CAD8197637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X9F9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8197637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="46" mass="5562" checksum="5649FCC7945402BA" modified="2022-10-12" version="1">MKTVNNRIKPLSKKKDFSTQLNTKIKMHELITFDDIHIRQEQHQEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XAD8</accession>
    <name>A0A8S1XAD8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1170086</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000117">
        <property type="protein sequence ID" value="CAD8198067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XAD8"/>
    <dbReference type="OrthoDB" id="2747330at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05471">
        <property type="entry name" value="pepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000217">
        <property type="entry name" value="Chromosome 16, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000201">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034164">
        <property type="entry name" value="Pepsin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF51">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="356"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="5">
        <location>
            <begin position="22"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8198067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="419" mass="48604" checksum="9CF9E67CB0198227" modified="2022-10-12" version="1">MIILIIATVSCLDINLTNLQFNYYSLPILANNNTYNLIVDTGNEDMWIFGKNSNRRNYFECQNCIPIENHTLEYAQGKISGFKFNQIFSLMNQSLILQVIEASKVEHFYSIIADGAIGLSRKQLSKEDNFLKKLYESKVIDDIQFGLLLNEQNCQPTSILILGTPNKNYYINELQYVKSIESEQWTVKGKSIEIFGKNNKYELESYSQIIVFDSGISKIQISKYKLNKLIAILNENYQLNCYKETQNQINEIVCSYNEESFPELNINIDTNISLTLLPQDYIAYCHYNYFLQHICYLNFQQIDNKDVLVLGSVFLQKYYTHYDVNTQQIGIAKSIYYQRDKQSLNQLGSTYIYNDVLFFSFILVLLLLIGIYFSLKLLLKQSIPLYLSVLEKSSSRSQEQEMEAITLKTQSNSQPPLTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XCA4</accession>
    <name>A0A8S1XCA4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T1190080</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000119">
        <property type="protein sequence ID" value="CAD8198598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XCA4"/>
    <dbReference type="OrthoDB" id="297794at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14003">
        <property type="entry name" value="STKc_AMPK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000740">
        <property type="entry name" value="SNF1-related protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="18"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="421"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="527"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="485"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="96"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="145"/>
            <end position="146"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="8">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8198598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="651" mass="75541" checksum="77BA7AB6A4DEC598" modified="2022-10-12" version="1">MPKLYTFKDPNFNPLQNYVVDKTLGQGTFGKVKLGIHKCTNEKVAIKILEKEKIENEADYVRIQREIHILRKIRHPNIIQLYEIIESEIKLYLITEYAPGGELFEYIVSKSRLEEREAGRIFFQLLNAIEYIHQLGIVHRDLKPENILLDINKQVKVVDFGLSNLYQPNQKLHTPCGSPCYAAPEMVSGLSYEGLKTDIWSCGIILYAMICGCVPFEDQNTKQLYEKIKHSDYKLPKSVSPQAADLLRKILQKDPQKRITIPEIRQHDFILFAGKMTIPEGVNTKLDNFKIDVDYTILQQLLQYNISEEEAVQMIKNNKHNCISTCYYLIKLKQQREKQIKPDNITTKPISIEKQNLFNKTQLIEQQKSSPLVKQKLYSQQQPTQPYSQDVQQQILLHLLQNQQQQQLIQQQQQQLIQQQTQPKEVKQPQVSKVAETGGSSSMEMFLNKAFSDRNQNKSQTKGKTKSQDPVPHRDISAPKKTSLPSKNQKSISQKPSIQSKDQLAQLLAVKQSKYLGNTDSTTYVRHKSNLKFKQGSTERSDRHSSNSQRSEITYLKRSVENRSSQHIKNDRKTRVISTYAYNDTQKPIQTQPNERINTDLSKEQSNVLQSQQQRLTHMDNLALGIQIYKHNIQNRFLIKQQQKRKSKQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XFH0</accession>
    <name>A0A8S1XFH0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ZZ-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1220086</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the MT-A70-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000122">
        <property type="protein sequence ID" value="CAD8199663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XFH0"/>
    <dbReference type="OrthoDB" id="10262526at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036396">
        <property type="term" value="C:RNA N6-methyladenosine methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02249">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000426">
        <property type="entry name" value="Putative N6-adenosine-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.90:FF:000029">
        <property type="entry name" value="Putative N6-adenosine-methyltransferase with ZZ-type zinc-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007757">
        <property type="entry name" value="MT-A70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829:SF2">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE MT-A70-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05063">
        <property type="entry name" value="MT-A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51143">
        <property type="entry name" value="MT_A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="7">
        <location>
            <begin position="533"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="domain" description="ZZ-type" evidence="7">
        <location>
            <begin position="596"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="201"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8199663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="715" mass="83195" checksum="9E59D36E4DA3F1EC" modified="2022-10-12" version="1">MTDQEKSSDVRLVQDGQKNVLKQKTGLKQINLDNLVYNKRERKQTGGPALLAIPQIQQKKIIQNKQSYKKKNAELHTPPREEVQQVQVPTLIKQQPISCQQLVFESDNSSDFDPKLKAKKSKKFKRTHKRLEIEDSSDEEQYKRRLILKRVNKIFKKSYEQKQILASGSEQENLVKELFKNVFVKIFQDIPISAAELKQLSNPNPEYSTGQISDGKQQDEEIKPEEEQIRILTEDGTDFETKRNLKLLGIENSEILSKLGEVKSYINCDIRYFNLDFLVEKVGGFDVVLMDPPWRIKGGQQNDSSFMFTNSKFSLDYNTMSNQEIMDIKIEKLSKKGFLFLWILNTQLNIAYEMASKWGYEIVDQIIWVKLNPQGNNVYLSTGYYFMHSFEICLVGYKCPPGEHVEYHSKISNNIIFSSVRNKSQKPIEMYEIIELMMPGAKKVEIFARNNNLRHGWFSIGNQLGETYQKWLVQISCNICSIALQPGICRYKSKKIANFDICQECYNKKINEGELNENEMFFLANKAEEEVLHQYHQCNRCDQNPIWGPRFECITCDNYDCCEACFDNLLQSGDPTHKDHDFKVIELPTFAEGIPCHDAKCNGCFQKPILGVQFICKQCTNFSLCQNCFFSRSQSELNGPRHKADHRFEPIYEVQNFSKKTYKCQGCELEKAGCVYKCENCFGFYFCEECYALKKDDWKCYVATSHKNYHTFIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XFJ9</accession>
    <name>A0A8S1XFJ9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1220085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000122">
        <property type="protein sequence ID" value="CAD8199661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XFJ9"/>
    <dbReference type="OrthoDB" id="10249567at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000260">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24413">
        <property type="entry name" value="SPECKLE-TYPE POZ PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="115"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8199661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="315" mass="36804" checksum="7478BEA40ED4332B" modified="2022-10-12" version="1">MLKKKGSEQLDIIFEAGEDLLNFSKNQVIMLKNNSIKLPIIRPRTFLSYSLEFCGITSLNYKMLRFQSLGRDIRPETSIMTSCLVKVIHTTEFSQMAQNPLKTSFQGLFIQGSHSDILLQINEESPIPLHKCILACRSPKFNGMFSSNMIESNQTLVKVEHKKPDLFKVMLQWIYCGYWKEFPEKIEDTCDLMLLADEYLITDLKQKCEEDIISKLDSSNILKILLFVEKHSNIISQLLLEKTNSMFIDDFDKIHKLNPNLEQDITRVPGLMTKLFQNLHQKKIRKGRKVHFVVDDFEESDSDDYVRNYTQHFYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XII7</accession>
    <name>A0A8S1XII7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sodium/calcium exchanger membrane region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1260069</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000126">
        <property type="protein sequence ID" value="CAD8200945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XII7"/>
    <dbReference type="OrthoDB" id="407410at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051359">
        <property type="entry name" value="CaCA_antiporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12266:SF0">
        <property type="entry name" value="MITOCHONDRIAL SODIUM_CALCIUM EXCHANGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12266">
        <property type="entry name" value="NA+/CA2+ K+ INDEPENDENT EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="349"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="371"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="400"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="440"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="474"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="515"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="549"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="7">
        <location>
            <begin position="81"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="7">
        <location>
            <begin position="406"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8200945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="573" mass="65389" checksum="1E333C9710292C27" modified="2022-10-12" version="1">MLEHLSTAPYEPKILSCQKSDFLSATEHSQQCYIAQQYCQHDFLYFNYSILSYCWLNGSIMTTLMVSGFVGYLVYNGISEIIKEFLIPSLEAVKVKFDVSEIMAGVTLLAFGNGAGDVLTALVASSYPGGIDYNIGATMGAGFFLCSIGVYLITKTSKSQIKMDPVHFWRNVGFQIISIFVIMVFGVIGQISYFSSISLTVLYLILVSLVYYQERDKILKSRKDSLGERLQTVHEAKYDLEIAQKFDDMKLLYLWDKKSEQELAAKPVKWATPLIRLNYVTGDARVKMLAKKFRFAVQLTMANIETQRQKWERLSLYEKIRAIIIYPLIQIFKYTLPGPKEDQFDKNQAIIFPIPGSIFFVSVVFSFPHWWVYFLALLFGFGISIFLNTTIPSQKTPPKYFFYLQLYCILGSLVWIFFLSGLLIDFLQFWGIITELNKTYLGFSLIAMGNVLPDCITLVSLAEEGYAIMALNGIYFGQMFTNLIGFSVAFLKQNFVNNGPIKFNLFSIIDIEQNAFKLMVILAAFLNLLFTLIMAVRNHYIISKSIAKLLTIFYCVFFTLSSSSAFYHAWTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XIK8</accession>
    <name>A0A8S1XIK8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1260108</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000126">
        <property type="protein sequence ID" value="CAD8201021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XIK8"/>
    <dbReference type="OrthoDB" id="297641at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13188">
        <property type="entry name" value="PAS_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="6">
        <location>
            <begin position="145"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="358"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="600"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8201021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="728" mass="85454" checksum="9DCA28D612985F40" modified="2022-10-12" version="1">MIIRAIITESQYRIHYLILMIFLQPLNHYMFYTLQKLECTQQTQTQFKQESIIQQNEQLICLSNDSKIDNLKCDLNESVIVNQINYDLESPKIQQEHKSIVVNRETIQQRIIIKKQPINMQKTTTNSSHSFQHDPLKLESHIDSKYSDLVAIMSNLPFGIIFVDQQLKILDYNQKVIQLLGITNPDNIIPFLDEAIQKSGEIQEVRSAKKIKKSMNKSPKKMTYFKQPSISLQKRQFDDQIPDVVSQYYNCGSQPRMDNESQQEGNLKSVFQKFQKIHQSQQTSTSRETYQFIIRLDSMNASQCKSKYKSLKLKIFQIEENGKWNQNVYLIVLENITKREEYKLLNHKYKFQQALLNSLCHELRTPINSVLSQLYALKDELTIDLLESHLNPAIVSTKRLQYQLNDILDYAQIQCKALTLNKSYFKLEEIFQQLQELFYFECIQKQIQLIIDNDHQIRLYTDKERLLRVFINLLDNSVKFTDKGGTIKISIEILKDQYKFIIYDDGQGIKDEIIEKIEEEAEILFQDSIQYNSNKLGLGLRISMILSKYLYKEQKFKIDSKFNEYTRISFGLSNLIENCSLEFQMGRQLSRLEKYCECSKILIVDDIICNHFALQVLLKKFKVKTDSAYNGDSAIELVEQRLKQQCCQTYRIIFMDIEMPQKNGFQASSEISQKLKQNSLHDECIITMYSAYSGDDDVLIATQCGMKERISKPTDIQKLEHIINKYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XMF5</accession>
    <name>A0A8S1XMF5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1280117</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000128">
        <property type="protein sequence ID" value="CAD8201642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XMF5"/>
    <dbReference type="OrthoDB" id="302007at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8201642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="580" mass="69898" checksum="A9E7BECD153CA8F7" modified="2022-10-12" version="1">MLQKQKQEEFNTKFFARAKKGMLKRFFFYDRTLNYIETLIFTEFSGRKQIIPMRNTQCLKPTCIYDIDELYERYEKSNYSSYANFMCHTCRKMIDLQGFYMDEDLRKIIDVIWNTYNKFSIQCQKAAIFKDGSWEAVLPEYLKRMYRKNVEEYDVKQNDDDFIDQPDEILKFELPLLHEFDVQEYAELLEQEDRGDERHHDSDILLRRYNLQIKWGEFAQFKNQVAVSINVMGIFMHYLHDYLEDRKNTMKMQAKLKLGVLCIKVISYDRFSKRAEFFFLSDNLEGPKANYTEYEQVIIAIYYDERWIALICDIEARRLKMIDFLEETIARTTLDEVYELGQYLISQIFQPYKFPKNPSRELLIGQIQEDMRNEIPRKIQLYCDCGHHVAQYFYYYILEEIELIKIQQKWPQDVGVKQKIHNVKYKLGEQDYFTAKICWVLLKMSQNTGFKILVYDMKPKERVEKDTLDDFVIEQARLKAEKLWDDNNKQPKQTTFQIVHGLNQVEDVVISKSGIDKQEDQNRKILGSRKWNLIQQLKKFITQLCIIIIIIKGQFFVEQFEFSVWQLRQTNSETLIINIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XNW3</accession>
    <name>A0A8S1XNW3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1320083</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000132">
        <property type="protein sequence ID" value="CAD8202860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XNW3"/>
    <dbReference type="OrthoDB" id="285022at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8202860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="101" mass="11692" checksum="DC127876A2B32CBD" modified="2022-10-12" version="1">MSSEKDFQYDIHDVQNIKTAFLDLQQEESTGFKLGFGTAILSYFVFRRFTYLRTGPRFVGSMILGSQVYGFYTHRSRAYYDYVAQQVNLHASEAINQCLGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XPC8</accession>
    <name>A0A8S1XPC8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T1330044</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Plays a fundamental role in microtubule organizing center structure and function. Component of the infraciliary lattice (ICL) and the ciliary basal bodies.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the centrin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000133">
        <property type="protein sequence ID" value="CAD8203131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XPC8"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000077">
        <property type="entry name" value="Centrin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000070">
        <property type="entry name" value="Centrin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF59">
        <property type="entry name" value="EF-HAND SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="25"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="61"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="98"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="134"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8203131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="169" mass="19825" checksum="D193947029616007" modified="2022-10-12" version="1">MNRNKQQPQRQIAPQRKARSELNEEQKQEIKEAFDLFDTDGTGYIDVKELKVAMRALGFETKKEEVKQMIKEVDREGKGVIEFPDFLELMTVKMGERDPREEMLKAFRLFDDDNTGKITLKNLKRVARELGETMTDDELQEMIDEADRDGDGEISEEDFIRIMKKTNLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XQ20</accession>
    <name>A0A8S1XQ20_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Band 7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1330022</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000133">
        <property type="protein sequence ID" value="CAD8203087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XQ20"/>
    <dbReference type="OrthoDB" id="2105077at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13437">
        <property type="entry name" value="SPFH_alloslipin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000004">
        <property type="entry name" value="Putative membrane protease family, stomatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043202">
        <property type="entry name" value="Band-7_stomatin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264:SF19">
        <property type="entry name" value="AT06885P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264">
        <property type="entry name" value="BAND 7 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Band 7" evidence="2">
        <location>
            <begin position="54"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8203087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="281" mass="31807" checksum="39C2C99E1427E00E" modified="2022-10-12" version="1">MEQQPLSNNRQNNEEDQLTQKRELTSYESCLDCCGNVSGCLRAWLPCIFCCCENPFYAVQQSSLGLVEKFGKYHRSLPPGLNQINPCTDTVIQVDLRTRVLDLDRQIILTKDNIQVNIDTCMYFRIIDPVRATYRVSRLTQSVKDMTYAALRQVCGEHQLQDLLEHRELVQDSIEAYLDKSTEQWGIYIEEVFIKDMVLTPQMQSDLAAAAKNKRIAQAKVISAQADVESAKLMKEAAQALDSKAAMQIRFLETLQLLAKGPSQKLMFLPLSPESQGAHNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XQB2</accession>
    <name>A0A8S1XQB2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1320097</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000132">
        <property type="protein sequence ID" value="CAD8202888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XQB2"/>
    <dbReference type="OrthoDB" id="283861at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010321">
        <property type="entry name" value="DUF922"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06037">
        <property type="entry name" value="DUF922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8202888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="249" mass="29591" checksum="5CC4D3594170ABA8" modified="2022-10-12" version="1">MNSVRQRENRKLSQSQRSQNYNLPIRASSQFKIHHLPSISSNSTCCSNSCTSSPPQSCSKLDEDNVENILVSKNTCQDPLSCFNYDDFKGVLPVTINGIKILKIDWSYFTASPNMQSPWKAHCFWTVGYTFDINMRKMKRSQNIRYRLIIQSWCCLNNKSWVKNKWDRLLEHETGHYLIGCLCALDFKQKADKFKYTKNYRMECTKLFQDTFQFYLQMEKQYDEETNHSQNVVKQKEWNQFIKQELLKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XSK9</accession>
    <name>A0A8S1XSK9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">DNA-directed RNA polymerase subunit</fullName>
            <ecNumber evidence="14">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPENT_87.1.T1360114</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="13">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="14"/>
    <dbReference type="EMBL" id="CAJJDO010000136">
        <property type="protein sequence ID" value="CAD8204251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="270392at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02736">
        <property type="entry name" value="RNAP_III_Rpc1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02583">
        <property type="entry name" value="RNAP_III_RPC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000019">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.274.100:FF:000008">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035698">
        <property type="entry name" value="RNAP_III_Rpc1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035697">
        <property type="entry name" value="RNAP_III_RPC1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015700">
        <property type="entry name" value="RPC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48446">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA' N-TERMINAL SECTION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48446:SF1">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA' N-TERMINAL SECTION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="15">
        <location>
            <begin position="212"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8204251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1368" mass="155937" checksum="3A2B7D7E050E90E9" modified="2022-10-12" version="1">MYIKLHDMKRKIGGLQYSILSPQDAINLAEVKIFRDKNYDKMTGKNIEHGPLDLRMGQSLRDTQCNSCTLLNECPGHFGYIPLQLPIYHLGFFTHVVKCLKCLCYKCGQIRLTSDEINQLTQKMIHCIQTRSYNAQLKVFKKVLKKCRSNKQCPHCFCEQIGEAKKLAKGDAGKIILDTMDINALYAWNQFINIPKQHQFLFCINPFLNDLSDLLTSIIPAPPNNIRPSKMLSNTTKADEDDLTMKLKTILYHNDLLQKQIKEGKDGMSILKTQFLTQAHYFHYFNSETPRLPQFSSKDGKVDIRGIYQRLKGKRGRLRGNLSGKRAEFTARSVISPDPNLAIDQVTIPQHIACILTVPETVTALNIQKMRQYVLNGPSIYPGAKFVKLGGVNYNLLFARRAHLAYKLQIGDVVDRHLLSEDIVIFNRQPSLHRISMMAFRARVDKWHTLRFNECVCTPFNADFDGDEMNIHLPQTYEARSEALILMDVKKNLKTIKSGESLVCLLQDFLTTAWLITNKDVFYSREQFMQFCAAFSDSNEQIDLPAPTILKPKQLWTGKQVINSLLVPNRKTRLVLNLEVKESNAFKGKLDYLWMDPGDGYVIFDKCELVCGNIGKKVLGASKLGLFYALIRDNSTQIAASVMQRFAKLSSRWISHYGMTIGIGDVMAPKSLIEQITQNTEQSYLQCKHYQRSAIDADPGLTVEQTLEAKVNKTLSDVREQVGSKCQEQLKSDNKVLIMYLCGAKGSNVNVAQMIGCVGQQVISGKRVPEGFTGRTLPHFKDCGDRYPNLDILHPKAKGFVKNSFYTGMDAIEFYFHNMAGREGLTDTAVKTATTGYMQRRLVKMLEDLHIAYDLTVRSCDTKEIVQFRYGEDGLDPLAVEDAKEVIKLQNIFTNSFTVLRKNQILLCKGDMLMKLNELFMIIEERHKKFLENYEATYEKEKKRVLALFQNTNQLVSEKQLQWFIRSWMERFLSMVMAPGTSVGPITSQSIGEPATQMTLKTFHSAGVAGMSITQGVPRLNEIINASKDIKTPQINVKLISRQIMEHAKLFAKSRDPQYQYAHHAVLQSAQQAQNVNIQIEQTKLMQILKRMDEIYTADKCYIELELDLHCLTQLKLEFVTTKYVCDLILKELKLPLDDVKYDKKIIRIAVRKNPKKKQSDNLLFLIKELQRKLLKLHIYGCSNIQQTVIQVTDKDDNENVKGEARLFASGIGFKEILRLDNIDWKRTVSNHIMEIQSALGIEAARQSIVNEVYTTMQSHSISIDIRHILLLAELMTFKGRILGFTRQGIDQLKNSTLMLASFEKTMDVFFNAAIQGKQDSMNGASQRVIIGQNVPQGTGMFDLMFPVKENIIMESSLNQQKFLFASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XUY8</accession>
    <name>A0A8S1XUY8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1390014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000139">
        <property type="protein sequence ID" value="CAD8204857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XUY8"/>
    <dbReference type="OrthoDB" id="10411488at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332:SF175">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="633"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="666"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="710"/>
            <end position="736"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8204857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="775" mass="91240" checksum="80FFFBEF802CFAFB" modified="2022-10-12" version="1">MPSLNNIKININQPPQCSKLYITRSNNLALTDLGVASSCDQSNDSPYTYQLRLFLREQDLTDFQQGSSDNSLILYPFQTQNQFLIQTPSPIDSSKIGILVQVLDDGGSMTQIFEQITVNFAKINCSSIQYQNLNLQKKISLLFEALNQKCDELHSQIYLNMLQQSILADENENILKFQALKLYKQLLIQSIQSKPQDNLPKQRCFDKNTKHFFITNNTTETEVNLTTKIESLKENTQNLNRTLNYFRNLKKKFEEDIQLNQYIWNEQIFQQYQNSQDCLRSLLFYLDDIYSNFSLINVKNEILYQAIMDLQQFMSLISEETQNTIIVNQKPLAINGNEIIWQIKRRTISQFNQQFDLEPAKEDFLVEYVQFESIYFQTNPLRFTSDLQGLLYAQFNDQTLQIYSQKYYLTSLKNSYHNRFISFENFSSSYGTKFGSYQICSNTTQFLFEYEVQCVIRTIFGKIYQCNLQQEQNNDTIELTCEYNKFGEIFLLSSTNFSIANVNNTIIQISDLSVDSTSDNQLVLQICTSSLSFIFMAVYVVYRYKDYKEEQESPENEQRNLSPPNILMNRNFVYKGNSKVFKEKLKQIHQTISLFNYRDQSIQLSYRILEVLSQFNLYLTLSILEFQMLNNQILFICLFIIINPLIILIMRTFYKIIEAIYRFKKIAAIVSQLVLILLLMTPNLIMQVFYLLKYLLKYFRIIMQSEQYKMVIVFVGNIIISQLLLEPLTIFGRIIIYRLIASSMKNMELNPVFHLMHFFVMHSSLEEIFEDFARI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XV31</accession>
    <name>A0A8S1XV31_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1370024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000137">
        <property type="protein sequence ID" value="CAD8204358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XV31"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8204358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="41" mass="4834" checksum="9FF004FB161AE7DE" modified="2022-10-12" version="1">MFASSTPRVTTQRTITFIQYFYNAYLFIAIDLLGVMLQVIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XYQ8</accession>
    <name>A0A8S1XYQ8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1410032</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000141">
        <property type="protein sequence ID" value="CAD8205568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XYQ8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8205568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="99" mass="11571" checksum="D66DE0ADF4A910E5" modified="2022-10-12" version="1">MQFQSAFELSKVNIFIKQFSKINLPVNLYIKMNTNDKIKGSEQLNINSIEISEEPQTVIWNSQCNNVIQIFVDKSFGNQMSYQTFQKYKSDLAKKILQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y0F4</accession>
    <name>A0A8S1Y0F4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ABC transporter family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T1430049</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001172">
        <property type="entry name" value="Cystic fibrosis transmembrane conductance regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000151">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002273">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF456">
        <property type="entry name" value="MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN LETHAL(2)03659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="278"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="350"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="468"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1002"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="251"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="592"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="883"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="1193"/>
            <end position="1424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8206138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1463" mass="168972" checksum="232EB54CE89312EF" modified="2022-10-12" version="1">MNSNLYSSTIREIKEERQCITKLDCLVNEINIMTPNDVEKQCLNSLKDPKKTSNLIESTNKQLQRKDSIATKIFKNVERLKKEKQKNKDFRINSQSCIQPENEMQFNQRRVVSLENDNKQKSHICQISIPKSVKQLKTDLFKAKKQINIRQVPKLKERCTFIKELFFISTIKAIIIIYQNIIKYHKTVDIKDLPQLSSQDQIEQYSAEYVNQMRQDLKLKIDFSFYNLFFWKFNSCFTLTMLILGMIESNSNFIISLLLYNIIENVQDGNKGAAQLNALYLTFLSIITIIGRHYQYIQLSKFGGKLRQILMYILIEKISTLSNCSIQKSNSGSILNIICSDLSTLEWQYGYIYCIPIVPFSLLCSGWILWLQFQGPYGLMAVLICVITYPTQTIIQKCIQTKIKLLKEYQDLRLKQQTNFLDNIQTIKMYCWQETVRQMILKTRFLEWKQHIQIQILTLIDRSINQSINIWGSFLFLIIIYNLGIPLNLQKVILTIQLMGSIRYYCVHQVSYGLKAIINLNVIFKRIKDFTKIEPLFAQILEFQNDHPNVNAQSYLELSSNKEMSQFSLIKLSEKANQQTKPSFQILQDEAISIRSVYCYWELQRLPLLKNINFIANRGQLISIIGKTGSGKTTFLQLILKEIPYIQGQLEFTDNLTFGYVEQEPFINPTTIKDNILFGREYNKELFDEVLKASNFLSDLNDLPESENTVIGDNGATLSGGQRARLSLARALYAMPNVYLFDDPLSAVDSKVAKHIFSKAIQQFIFQYQPTKRNNHPNPIVIMTTHQLSYALKCDYIVVLEDGQIICQGRASDVGLQIEKILNVQPVEINENSQNNRQQLSCDGKKKSNNFNIYYNQTNLLTPNEQVIKTIKKLILYVTRSKLLLILSLNILTEIIRNLFYRLLTLNADDEGIKKYALMISILVITHLINNVLKYSICSYVILDMNNEIFSKSMQALAHGKKSFFDKQPSGIIINKYSTDLSILDNQMPIVLIDIFEDGCQFIVSLLMIGFLQPFFFLPIMLTLFLSYILFKTVVQILSQLKINDLSQRAPQLSQFKIYIQGLTHYRLSNQFNQLKQKFFILANNSFQSNYIYHQTQKCFSQYVDLIALFTKTSGTYLIIAFIEDNSLFSQALLLLQSFHAQNTLLRQLMELSGLFNSYERLLDIQQIDKEKDDETISTVLPRAWPKQGSIVLKNLSLQYYANQQPVLKNIDLEIKPEEKIAFVGRTGAGKSSLISALFRLVDIGSESSILIDGCNVRELSLNQLRRALAIIPQQPLLFYGSIKQNLDPQNLYTDLDIWKALSEVNIDGMIEQQKQLLNCNVMDLNLSAGQKQQLCLARVILMRRKILILDEASANIDILTDEAIQEIIRSKFMDCTILTIAHRLNTIAYYDRVIVLENGQIAEQGNPFELLSDDYNAECINKKTKFAEMVLETGNKNAQQIFVMAKESYYIKQLKKKQKSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y2M2</accession>
    <name>A0A8S1Y2M2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1420053</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000142">
        <property type="protein sequence ID" value="CAD8205894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y2M2"/>
    <dbReference type="OrthoDB" id="289047at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398:SF0">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8205894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="485" mass="56189" checksum="AC073ED9B906D18C" modified="2022-10-12" version="1">MNQVSENFKRFDIFGQEIRLLATGQSAFRTNIGAFMTLALFTILAYSCNSFILEMNKGKNAILNSKDAVISGEEGYTFNSSELIFAVGLLDNLGQPIPNDNNRVFTISYYYCNKSLNETACIYIPGTICGNRIQQVSQQLNIPKGYEDITYCMDEEYIKQNPEIRIQGSTRQDNFTLLGALVQRCQNSTDYNDCAPSEDIDQYIKNANLYYSYSFHQFNKELDDSPYENAQNIDVTPMYYKVRKYIKIYFQYSQSQLEYNPFYFFPSYAQHDGFEYQNTAIDTALNFEDDSSFAQLEINLDAKKRIHFITYQTLMDVAAKIGGFFTIIRVIFEIALFPIQSILYRLYLINCLTHQQHNINTNTCDTPKNKNTNILTFYRLIKSGKARQFYLNQSLMIDKFLDITEILINPLRFTRQVEDLYGSIKKFDIVSARQNNFNINQQIDELVANNNNEFADFKSMSPRTINQEMKLGSLTNFKVPQLSIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y3H7</accession>
    <name>A0A8S1Y3H7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1430133</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y3H7"/>
    <dbReference type="OrthoDB" id="310096at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000592">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF206">
        <property type="entry name" value="HSF-TYPE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="4">
        <location>
            <begin position="3"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="101"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8206304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="215" mass="25372" checksum="D08C6505D6BCD2A9" modified="2022-10-12" version="1">MGNQKKFLQKLFHILQSKQHEDCIQWSQDGNSLLIISAELFEVMVMPSYFNSTKISTFYRQLSQYGFKVKQNENRQKQFYHSNFQQGNIEKLLNIEKRKKLQRHLDYSNSLNEENKKLNNELKLLQKQQLVIQAQLNIHTKIYLQICKQITTISNYFFKCNKDSQECTIFFEAIISVFKGFNPEIASNIFEDLKNIGGPLSPQLSPLLFPYLKFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y6T0</accession>
    <name>A0A8S1Y6T0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sperm-associated antigen 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1520007</name>
        <name evidence="4" type="ORF">PPENT_87.1.T1530111</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000152">
        <property type="protein sequence ID" value="CAD8208735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000153">
        <property type="protein sequence ID" value="CAD8209191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y6T0"/>
    <dbReference type="OrthoDB" id="7537227at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000357">
        <property type="entry name" value="HEAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23314:SF0">
        <property type="entry name" value="SPERM-ASSOCIATED ANTIGEN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23314">
        <property type="entry name" value="SPERM-ASSOCIATED ANTIGEN 6 ARMADILLO REPEAT-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02985">
        <property type="entry name" value="HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ARM" evidence="2">
        <location>
            <begin position="124"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8208735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8209191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="506" mass="54768" checksum="828BD2EA0D62A22F" modified="2022-10-12" version="1">MSRAVLQPFEVYQKARVQFVQTVAELAKRPQNIEALQSAGVMSLLRPLLLDCVPSIQQSAALALGRLAKHSEDLAEAVVSNEILPQLVSSLGEQNRFYKKAAAFVLRCVAKHSSTLAMAVVNSGALEALVQCLEEFDPSVKEAAASALRYIAKHTADLAQAVVDAGAVPLLVLCIQEPETTLKRVSAGALSEICKHSAELAQNVVDAGAAPFLSALIPHHDAELKRSVCFCLANIAKHTIDLAEAIVDVDIFPKILYRLKDTDPGVRKAAATCIREIARQSQDLAKMICSAGAVVSIVDYINEAKGDARLPGIMTLGFIGAFDEALAMGIIAAKGIAPLKDALIKEPDQSVKSASAWSLGMIGGHSADHSRAMAEADVPSHLLAVYKFPESSEDLKKKSSQALKSILQMCTYLPALEPLISEAPPDILQYVLHQFQKTLPNDNAAKKQFVLSEGLKKIQEIKANPGSKLRQYIDEINSYYPPEIIQYYSAGYEQQLLKKLDDYNAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2024-11-27" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y758</accession>
    <name>A0A8S1Y758_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1570003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000157">
        <property type="protein sequence ID" value="CAD8209895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035867732" evidence="1">
        <location>
            <begin position="22"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8209895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="329" mass="37893" checksum="A363DE504F86108D" modified="2022-10-12" version="1" fragment="single">MIQTFALFLQFLLCLSQWISSYTNLINDKNLTTFSQVGPSNSNYLVCNSPQIKYLTLNRDYPTATQKKQISLEKGNHISFELILQGTWQNGQIQFQFGAEIISFQYQTPNSFENFSIICDSYQSEIKTINFILQKSDSNIIKFTMSNEGNGQVSIRNLLISKIKCHYLCTQCNGIEYNQCTQCFYGTPLDGICNECPLNMVFRPGIGCSLFCNYQEYLDFNRHCQQYPSRQILFSDYYMVEEQVKMELILDTENTNHIPIYMSYVGRYDLLGVFRYNQGTKRLITEVTTNIGTYLIGIRTVIILYNEIPCGSSISLKINDTYAGSVYXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y7S6</accession>
    <name>A0A8S1Y7S6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Carbonic anhydrase</fullName>
            <ecNumber evidence="2 10">4.2.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 10">Carbonate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T1580012</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the beta-class carbonic anhydrase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="2 10"/>
    <dbReference type="EMBL" id="CAJJDO010000158">
        <property type="protein sequence ID" value="CAD8210115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y7S6"/>
    <dbReference type="OrthoDB" id="10248475at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015976">
        <property type="term" value="P:carbon utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00883">
        <property type="entry name" value="beta_CA_cladeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1050.10:FF:000001">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001765">
        <property type="entry name" value="Carbonic_anhydrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015892">
        <property type="entry name" value="Carbonic_anhydrase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11002">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11002:SF76">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00484">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00947">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00705">
        <property type="entry name" value="PROK_CO2_ANHYDRASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9 10" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="257"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="234"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601765-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8210115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="301" mass="34758" checksum="2F00CDDFDC2AF045" modified="2022-10-12" version="1">MQNQIVHESISEMKKYAKALQGNKNYVAKKTAEDPEYFTKLAQGQNPKYLLIGCSDSRAPPNEITETDPGEIFIHRNIANVVVPTDLNINCVIQYAIEHLKVHNIVIMGHTFCGGVKAAMKQDSVGGLLDLWLNNLKLVYEKHQDLINQLENEDDRVACLAHMNVREQVLNVWKNPIVQKSWQDGHPVMVHGWLFRVETGFIEELEIEESIPNNLSKVFKLNFKPTQPQPKFKQQIEEKEDERARSPSIRKRFQRMQSRLETEIKRLTITLQDANQEVEKEVVEQVSEVLQKDLDFTTDDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y7T4</accession>
    <name>A0A8S1Y7T4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1590101</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPP phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000159">
        <property type="protein sequence ID" value="CAD8210506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y7T4"/>
    <dbReference type="OrthoDB" id="5593063at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033192">
        <property type="term" value="F:calmodulin-dependent protein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097720">
        <property type="term" value="P:calcineurin-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043360">
        <property type="entry name" value="PP2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45673">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2B CATALYTIC SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="3">
        <location>
            <begin position="130"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8210506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="475" mass="55202" checksum="8D21F837380D9B59" modified="2022-10-12" version="1">MDLLKDPKGDRQVNTIPTPPHRPLSEELLFIDEKPNWKLLKEHLFKEGRITKSQLMKLVDMCNYHLKNEGNVIYVDDPLTVVGDIHGQYYDLMKVLEMGGDYVDRGSFSIEVVVLLYAMKIYYPTRVFLLRGNHECKQLTSYFNFRDECLYKYDQEVYEAIMASFDNMPLVALINNKFLCLHGGISPDLKSLADVERIDRFREPPKAGLFCDILWADPVENEDLYDEIHYRGNDTRGCSWFFGEKAVQPFLDNNKFICLIRAHEAQLEGYRLYKYNEKIKEFPQVLTIFSAPNYCDVYNNKAAILKFENCTLNISQFGYSQHPYLLPHFLDIFSWSIPFVTEKVTEMLNYVLQPRPGEKIEDSEDEIPITKVITGQQNMGQSQSNSRPSGKLQQITKKQIDITKSKISFVSKMMKMQTVLRQERENIIKLKGLCPDKRIPRGVLLQGAEGIKDALEHFNTTRAADRINEKWPETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y7X9</accession>
    <name>A0A8S1Y7X9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1550113</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000155">
        <property type="protein sequence ID" value="CAD8209691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y7X9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8209691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="170" mass="20494" checksum="7DD03A3953A38480" modified="2022-10-12" version="1">MISFTFYNPNLLVKDFILQFSKNLITYNSQLFSQYQNQININTQIQIYLIKEESSKSIQIIYNLSFQRIQNLKKKNSKGPFQYYIHLLLLISPKRICNSNLSNTISKEFKIIRILQNIIFGINSQILNRVYFIQRNNDRRIEFQNQSLRYGNHFIKFNRIYCNSQFLKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y893</accession>
    <name>A0A8S1Y893_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">LITAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1580088</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDIP1/LITAF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000158">
        <property type="protein sequence ID" value="CAD8210265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y893"/>
    <dbReference type="OrthoDB" id="305631at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006629">
        <property type="entry name" value="LITAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037519">
        <property type="entry name" value="LITAF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292:SF6">
        <property type="entry name" value="FI16602P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292">
        <property type="entry name" value="LIPOPOLYSACCHARIDE-INDUCED TUMOR NECROSIS FACTOR-ALPHA FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10601">
        <property type="entry name" value="zf-LITAF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00714">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51837">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="LITAF" evidence="7">
        <location>
            <begin position="71"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8210265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="158" mass="17795" checksum="17FFC1773CE2A050" modified="2022-10-12" version="1">MQQDTNTTFEIQDGQQQYQVPLQTDNQQVYTQPMNNYASQPQYTPNPQQVVRVQGSPYPVQQELVVVNNQLANATQSQILCNSSRFAILITCPYCQKQGLTRIDFKAGSGTWCCCFILFLFICCICWVPFVAEKCKDVNHLCSNCGQLVGSCPYKVCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y9A5</accession>
    <name>A0A8S1Y9A5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1620022</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000162">
        <property type="protein sequence ID" value="CAD8211066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y9A5"/>
    <dbReference type="OrthoDB" id="311290at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="26"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="679"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="791"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="309"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8211066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="817" mass="97626" checksum="233E8C691A5D6DB9" modified="2022-10-12" version="1">MRSNIKQTHYVLDFSDIEDISDNISESSSYQHNQPPVSPPRSDIKSLQQGSSLQPVKKETVEIPSLIIQHEEDQIPRRKIDNNLIQRWEVNFDELSEYEEPESTPESIQLQKESSPPVEEPQYIEQFFEDQQIIDEVEIDQDQLILERPKDVQKQTLIMKQEFQLKDKLNANILTNPINLSQKEYLNDCNTITPKKDINQDIQISKCKQIVKQKFYNPKNSLIYLRLRGREDLYKRNEIVVDETQKPQEVDQLLEGLDCINNLLIDLSNVKEKKKNEVDKNKQVPLQIQKHRVDQNSVNNTEKKSIKIVQIKEQKQQENQLEIQKLQVQKQLESAQKQQLQRQIESAQKQQLQQEAALARQIRKEIQQQEKEIKKQQEQKQVYKILTKKLINQGLPLYRCLLRQKQKDFIKWLRFSEIALINEGEKEIYRFEKRLSDQMFLQIHKQNMKSIIITKQYMFDPITLHYVKTKENHQENTSLEKEKQIKQDQDQKIISNKINSECKIQKEKKQSKQKKLTKQDSSDCVELLEEDQNKQSIINKEKGIQSNSDDEDFDLLDADRIQILKIVIDKRKYNNRQQLQQQNQLIQPSDEYKQPDDGVIVRQRIDQQNEHFQKEEQYPQIQQEQKDNLYLPVNISGQGINNQNKIKQKKHRNYKYKEQPLSRLETNDEIKKKEKIYNKRKEKKKLKLNKNNNNQRKKNKRENKKPYNNRIRINQELKNLEYFQQQQVNPKIQKDQRINESKIDRIIAKSEIKQDLQSCKLSYVARQSSVEKGIISINKLFAETQLKRLRRNETDSDDSDEFPQVERNRNYLKKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y9Z0</accession>
    <name>A0A8S1Y9Z0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T1630014</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000163">
        <property type="protein sequence ID" value="CAD8211306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y9Z0"/>
    <dbReference type="OrthoDB" id="290598at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08215">
        <property type="entry name" value="STKc_Nek"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="179"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="317"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8211306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="913" mass="107021" checksum="3361D7534ADD3A18" modified="2022-10-12" version="1">MLENIKQLLIPQSRERYGIYPNVLKEVFSQLSKGVQIYIYFTISKLLLTIIFLGEALKCELSESHSTATTLILHCIFLLYHEFKYKMELENIEKLSQIQQVLEQEGMNLEQIPDRQSFNKLMMTVKKSIFSQCPDLNRESKYLNFLAQVIIIRFFILMLDSPFNFNMFQYFQETCDANLLNVMFIMVLSMFLEFIFLYCLLVVIVIALPLLCCLSIYRKGKQLYSIRKLKKYLESIPPHKYTGLDDAWMKEDKTCCICMQEYVHNENILQLPCSGQHQFHESCVRNWFDLSNLQIIVRLKIKFDIYIIYIIDIYVKYKFCGKLNNYYNQRIFHSPWINLKESKKQAKAIMEMYCQCKENQMIRHSYYLLFQLFAIKRVDLSFRESYVVDPLNEVKLLRSLDHPNIISHYDSFTHNNKLCIVMEYAENADLSMMTNQAKQTQTYIDENTILGWFSQISIAIQYLHQLKIIHRDIKLQNIFLCTNGIVKLGDFGISRTLDNTLDLAQTSIGTPFYLSPEICQNQQYNHKIDIWMLGCTLYELCSLQKPFKGESISEIAFKIINEPHPKIQRNYSDFISQLIDEMLEKNPEKRPDISTIIQYPQIQSELYKLQGLYKTQFNYILPVSPMQSNKSSQKKMHKNSIHFLVEQSKQLQQQQQLQQQSPQVCKEKRRKVSLQQLIQDTSNQNSPTFKQLATADNANKNKPLSFNLHTLPDVINESIQQNSAVMAQRQLRYQKSISINTQIDDATKRGTNEYENENQPTLLKNPKTFSFAGQFLNPNAPTSPQRSILLTDFLKRKIGEEKFQQMKTLLESSNNPIKMLDQEKELVSEILGEENMECIKIFKVLISSSITPQAQHTRTKSSQSFQQYNHNDQSLIDLIKESDLIQNAEHSQNSVFEISFKNLQNCHDAFKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YDF0</accession>
    <name>A0A8S1YDF0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1660077</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000166">
        <property type="protein sequence ID" value="CAD8212085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YDF0"/>
    <dbReference type="OrthoDB" id="408964at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="30"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8212085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="283" mass="33501" checksum="36EBAA422BBC4CAD" modified="2022-10-12" version="1">MDSNLNQVRDKIYVIGSIKNKEGKIIEINFERGKIVSTTQFGDFYQFINVESKEVLIAKIIRKNKIDKIKFRQQVLQEIRIYQQIEHKNFLKFYGTFEDENFIYIILEEYINTLNDYLQEKGQFSESETKQFIIQIVDALNYLHENNILHRDLKLSNIYISKDYQLKIGGFNYAIRLEQKQERRKSICGTSKYLAPDILDNKNGYSFEVDLWYLGIIFYTLLFGVHPFEASDIKIAYQNIKANKLVYKEDVKISENIQKLIDELLNSDANKRVTLDMVIQILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YJ82</accession>
    <name>A0A8S1YJ82_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1810003</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000181">
        <property type="protein sequence ID" value="CAD8213558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YJ82"/>
    <dbReference type="OrthoDB" id="285236at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026306">
        <property type="entry name" value="RSBN1/Dpy-2/CEP530"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13354:SF11">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13354">
        <property type="entry name" value="ROUND SPERMATID BASIC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035851407" evidence="2">
        <location>
            <begin position="22"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8213558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="546" mass="63333" checksum="A1143583E10CEE59" modified="2022-10-12" version="1">MKKSSLIQVLFLLCLPQELYNLDTESLDCSPSLEQVSKSMEAWQEILSNPDQIENDIDQLQKLQIAVHDYRESEGQKKTLLEYHRTKGYCAFVEERDSFFHQWREYDQNQLDRQITLLDAASSQKDADQCCDKIGELINKILSEREQIRDQFKKSSIKINIINSQSDRVQNKVESCFQNGACLSHLNKGGASEFEKEPVSASALVSSSDSEDDEETLNKKQSSDFTEDKPIKITEVDEKGEDLLEYGYGYWARFMLAYPKFMPKGKNAPWYFVSRLSANKNTADLIMGDRLLAIWLGKGFYHFATCDQPEIQPNIVKNVDYPYRKEKSSCLYYIWRNDFKKIQHQVIHPTTKFVRFTVGGTDNKRNPGFNGLLSAIYFSTKKGVYIDNFEDMQAKLQSMKRIPKKIIPDNVTYKVTTNPISRKSEDREIHQIIGTTSTPKFPHEYGISGWFKWMPTNKQQDWHTIFRVQIQTPSTDIYLGDRTSSAWVRKSDGGNIHLPTYTITDLEGNGNANLFKNIPHKNRHNQWFFLYFGYSRPLQKAKAYIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YKQ7</accession>
    <name>A0A8S1YKQ7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1930002</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000193">
        <property type="protein sequence ID" value="CAD8214001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YKQ7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8214001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="176" mass="20668" checksum="05582AD1AEC87088" modified="2022-10-12" version="1">MNSKASQIRSIQRALNFRDHNVPFWTVLDVLEDQQRTSVRVQTYIQSLKDVQNYLMENQERWSEVFANVGLLKEIQQILIVDMSLIVYKDYIHELLVGCQQEQQNAITRRIGQKDSDSWDQSICLKRCKIKFIGAIQNSHDNWVNSICFSSDRNTQTEVIKINLTIYVMLKQLIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RYT7</accession>
    <name>A0A8S1RYT7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0020279</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="338608at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030621">
        <property type="term" value="F:U4 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002141">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19846:SF0">
        <property type="entry name" value="PRE-MRNA PROCESSING FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19846">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25173">
        <property type="entry name" value="Beta-prop_WDR3_1st"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="33"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="85"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="127"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="169"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="211"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="253"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="295"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="337"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="379"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="421"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="463"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="505"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="547"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="589"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="631"/>
            <end position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8133196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="759" mass="83434" checksum="972381FAD3808023" modified="2022-10-12" version="1">MLKLVQQKFQELFNFFVQFKHLEIANLLIFKNFFPDGNTLASCSRDKTIRLWDVKTGQQKAKLVGHTKNVKQICFSPDGNTLASXDGHTNTVYSVCFSPDRNTLASCSRDKSIRLWDVKTGQQKAKLVGHSCRVRSVCFSHDGNTLVSGSDDKSILLWDVKTGQQKAKQDGHTSTVCSVCFSPDGNTLASGSCDNSIRLWDVKTGQQKAKLDGHKSYVQSVCFSPDGNTLASGSDDCSIHLWDVKTEQLKASLDGHDETVLSVCFSPDGNTLASGGCDNSIRLWDVKTGQQKAQLDAHTRIVSSVCFSPDGNTLASGSDDKYIRLWDVNTRQYKAKLVGHSGRVWSVCFSPDGNTLASGSDDKSIRLWDVKTGQQKAKLDGHSSYVRSVCFSPDGNTLASGSDDKSIRLWDVKTGQQKAKLDGHSNYVNSVCFSPDGNTLASGSDDKSIRLWDVKTGQQKAKLDGHSNYVRSVCFSPDGNTLASGSDDKSIRLWDVKTGQQKAKLDGHSNYVMSVCFSPDGNTLASGSDDKSIRLWDVKTGQQKAKLDGHSSYVRSVCFSPDGNTLASGSDDKSIRLWDVKTGQQKAKLDGHSSYVRSVCFSPDGNTLASGSDDKSIRLWDVKTGQYKAKLDGHSDTVRSVCFSPDGNALASGNEDKSIRLWDVKTGSEITSTNKNYEDLLTYFKILLQQNCSASNYITTLFISQKPIFQAKGALILKGKFLNQSGRDLKTLFKQKGSCFLENIKIFNQKKKILYNENY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S1X3</accession>
    <name>A0A8S1S1X3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0030208</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S1X3"/>
    <dbReference type="OrthoDB" id="302846at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="229"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8134248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="612" mass="71179" checksum="91E43D09CA0AAB25" modified="2022-10-12" version="1">MLSNDRQHFVKLVRNPQINAIQQLTQLVTQASRTKSTLQPSTKRIAEVQKYEKIDKQEKIIHTTREINSQQSIRNYFDNSTCKNNKENLPSKKHLYQENRSQTPMAQKSPIVINTRGRGSVSPLRCVQQNQITKSQNKSAFKQTEDPQIMDMVDNLKQNILRDLQEVTKRYQSNNPKPIEMLQKQNQKIKNLLDYKETQIKVPYFDYSSQKSIKHPEIFYYDENSQNKQQFLEQQSQILQQQLEIIKQQQEALRQQQVSVLNSKNNFNNFYNQMKKPQNESIEDIYGKSQYTISNLSSIIPIEASANQNKKINVSFESSLEQNPINLQLEIIQGNDCRTTFGPSQLESLIKSPTQVEPELFSEQQVMKETVKYVQLDNSQGKVSSAKRYSNDDQELVEQQELIQQVPPPSAFSNKSNRYDQLMQNFQSLRKLSREPSFVIETKKSVQMNELNHISDFAIMGSQRESIALCQTKLITFQEVNNSQEECVQSPTSLLGKQQILTFEQDFEQKSIYSNQSQYDQRSIQQLLQKSSLSQQQGSSFQKQSISIQQSTEQTNRILESIRSNESFKQRTASFTEFLKVKDELPIKITNVQKLSVRPAISFLQYKQQNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S2F7</accession>
    <name>A0A8S1S2F7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0020561</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S2F7"/>
    <dbReference type="OrthoDB" id="304898at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8133695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="448" mass="53852" checksum="4B8E55DAA32B2AE2" modified="2022-10-12" version="1">MYEYTSRLAFSLNLIANKQKEIIDEFVTDSMFAVSLDERTRQFNLEFKNKTFENLKNIKDSEQIRQFLRTSYVLNNMNESQRINEKVLSKLQKLEEFCFKKIKTSESYMNAENQDYEIDIIQQDKINEKHKKMKLIVKLFNFGKPILIVIIKTEQVHNLIESYEKDILEYQQSIVNYSQEILNRQANINLEIKTIKQRFSKNIQDQMFLKIQCLNLQIMNYVRNYLIYFQQDKIPQISQIQYISNLFIYLDTIQQYLSTIAEYYSVKFIMQHQMEASFSLNINIKFLTQISINLFQQILMHSMKNQVISLNITEELQTQTKRYTYQKIETKKKVEECQTQSKQLIDLRLISFTFTFYSLHYVNLQKLQSFQMEMKNDINNFNHIPEYVTCYLLDQMGPNNFVQINQTEIIDSDGKTFYQNRLKFLIYSDQSQLEPSFFKQIPVLSKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S373</accession>
    <name>A0A8S1S373_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0030114</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S373"/>
    <dbReference type="OrthoDB" id="296944at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8134062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="212" mass="23932" checksum="283AF4F4762C22BF" modified="2022-10-12" version="1">MSESLNFNLKLYRERLADKYSKSPVETRFDSSINGVESQRKASIKKDKTNTPSLNNLVNKLQEYSNKKSKQNDSFLFKSGKSNQNSSPSKINGLHQKIQSISSLSPPPSQNNLQFPVKGHQYSITQFSGIVSPQKQSFISPTHFSQITDRYEPQESSTDISSLIPLHEIVNMRTKLENANINVANITPTYLTEFIKLANTIHSQLKVNKKQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S4V4</accession>
    <name>A0A8S1S4V4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0030224</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S4V4"/>
    <dbReference type="OrthoDB" id="283145at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41749">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41749:SF1">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8134280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="101" mass="11778" checksum="73017E104A8B2644" modified="2022-10-12" version="1">MSKGFNLNLKNSNVMTFEQENDLFAGQSIKLNFKLGDKQFDLEFKVGQDVEWAKKCVAEKLDCGRNEFDLIYEEKVMPEFFSLNDIPNLKDQSSVQIRMKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S6U3</accession>
    <name>A0A8S1S6U3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0040217</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S6U3"/>
    <dbReference type="OrthoDB" id="17328at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026508">
        <property type="entry name" value="TMEM164"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20948">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 164"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20948:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 164"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14808">
        <property type="entry name" value="TMEM164"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8135425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="277" mass="32679" checksum="4B89D7159C258FAC" modified="2022-10-12" version="1">MDIVRWGLYGNIYTETYEHFSDGGPKCYQDMTNTFRISISTISAIICILVMKKCWAKIKYSQELTRAMGKKPNIFEYLVGLACWGTFAVQTGYKLYTGRGVFMNNPCHIVLLMQGYVLLTKKHKFSAILFICQLRWLYGVFAALKFPVITGLELPLEVEFFWIEHLLGAFAGPLVLTLSGRFFFFEWKTFFIHQMFGWESHVLYQRIYMLPISLMTWANLNFMLCRPDHDPFVPYIGNWYYVFSEFYLNLVSIIAFVIVFILTWVLRKIIRYKEKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S6W8</accession>
    <name>A0A8S1S6W8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0040437</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S6W8"/>
    <dbReference type="OrthoDB" id="29755at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006818">
        <property type="entry name" value="ASF1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12040">
        <property type="entry name" value="ANTI-SILENCING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12040:SF0">
        <property type="entry name" value="HISTONE CHAPERONE ASF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04729">
        <property type="entry name" value="ASF1_hist_chap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8135818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="224" mass="26307" checksum="FA92A3ABC7407E5A" modified="2022-10-12" version="1">MALINITNIVFDQDTALFNSPIQMQITFDVMRQLDEEIEWKLIYIGSPNSDKYDQVLEQFSMPPLQQGTMQFTLMSSGPNFELIPSKDDLFGASAIILSVKYRKQEFFRVGYYVYNTYLEPELIENDPPQVLIDRVYRQINTQAPRVTRINIDWEGQMVQLYVNPQQNNQSFMFQQQPIFNDITNNPDYLQQSIQDKIQQPQQQQQQYSGGLNNNQYTDLQSIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S8K3</accession>
    <name>A0A8S1S8K3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0050224</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S8K3"/>
    <dbReference type="OrthoDB" id="48314at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 5-kinase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF263">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8136586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="592" mass="68759" checksum="DAFDA6A4704CCD0B" modified="2022-10-12" version="1">MDQSIVKLNTECEDIQQSFLSSPSEYFSNQKDDLVEKCQFSYKLFCETNPRDQIAQKIVNFQQEKQQLLYLIEKLEQYFIKDITTLLEYVEQLEQSYNTLYNGGFKIINEQLLTFFDYEKVQKLNETINLLEQRWNLPQIKETGKQFEKVLGLSSLKCMLEEHVRDQTMVQFFLQNQIHKPPVEYLFLRSKEVDKLNEQDGIYFGEVLQQMKHGFGVCIRGQAIWQGFWKHNHIVWGQYTFEENGQKKIMQGCFKDDMLEGEGKCIAFNGDTVQGQFRRSRIQGRGTYRFSYGNIYEGDLKNNTIDGQGSMKFANGDQYEGDWRNHSIYGKGKYTYNNGDIYEGDFVNGDKEGKGILRYENGNLYEGEFKNNIIHGQGVFTFANGDKYKGEFKNGQRDGKGKYEQVSGEFYEGSFVNDKREGFGIQKYSNGDIYEGQFRNDKREGQGRYKFANGNVYIGDFVNDKIEGKGKKKFVNGDCYEGEWSNQLFNGKGQYKFANGNTYIGTFINNKREGHGIYKFANGNTYEGEYKNDKRDGKGIFIYADGNREIGEYIEGKPSGEHQVYKGRQSSPFKINNYNSGKLDSTFITSRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S8N5</accession>
    <name>A0A8S1S8N5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0040393</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S8N5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8135730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="600" mass="71951" checksum="675A63332086B7A0" modified="2022-10-12" version="1">MGNNQSQNNQSKLKKKDDLIFLFNNNKKQNVFSKLSLKTLSLLTSFLSIQEYYNILLTSKETYQLFIQTSGCFQIECHRLLNINDNLMISQNWKKIIQSLLFIPIKALPFRESIKSVRQELKQIPILLEHKVAYSGFQKAILYFQNNSTFDNQVLQYKEYQESKQFAIQNQDLVLKVRQGLDVYIQLPSLLMQVYNLHDYIKIYGLYLEIKFCKCNLKQFIQLWNHYKSWISVLEQETDNIIAQFNYIINAAFPLQRLPIYTVRQFMVSQWLRKSDRGEIIVMLREEFRLKMIESRNKNIKFQELRDYVQYLIEISTNQINIHQYGYYNFEYCQEINTLVHLAVDLSPLLTINYQSDESMLIYIFGQYIYDYQIFELLFKFRIEQFKQQIKIHQQASQKFNQILVQKVEDQQSLHNNNLFDNQIQEFNYHLSMQYDVELKIKSLFKQISYSNDNICTISHNKQAESLQASKISLASTNCSSFFQSFIINDQLYHYVKFNQQELFNQIQLFYQRGIAISYYQQEIEPENNNIYDIPELGQYYELTFPQLLQLFSDKIQRSNSIQYFKRQISISIFDQQLNQHQIHTKQLSQTEQLLINILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SI69</accession>
    <name>A0A8S1SI69_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPENT_87.1.T0060447</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tim17/Tim22/Tim23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8138094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2261329at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10485:SF0">
        <property type="entry name" value="AT05822P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10485">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM-17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13771">
        <property type="entry name" value="zf-HC5HC2H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="16">
        <location>
            <begin position="165"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="320"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="327"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8138094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="357" mass="41516" checksum="483671480411CC9E" modified="2022-10-12" version="1">MAMREQPCPYRIIDDFGGAFSMGCFAGCIFYFLKGMSFAPKKERFFGGIQLLKRRAPILGGSFALWGGLFSITDQIXKGQVKGKVKHEDDEEMSMGREYQIGAVEKCGIPKLDQGQPPQGIKKQMFKLCNYLIFYNTLMCDSILPEPILRGVPLKKMQQGELKFPIRCAFCRLLKIRSFVQFYFRFDLVSRFGKLCLFKDKEGREICSHEMCLLWSNRVQVDYNTMLVDIATLIQAVQFAQAQMCRYCGSIGASLKCNKAECHVHYHFNCLKQVRLMGLQLDHEQKFRFFCEDHVRHNYQGQSDWFITRIRMKHIIQQGGQPAFTKKNPQIDWSTRDSGLNNDSDMEEQQFNKKHRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SII8</accession>
    <name>A0A8S1SII8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0070197</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SII8"/>
    <dbReference type="OrthoDB" id="306857at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8138602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="436" mass="52488" checksum="F4DAEB0B0DF67F8F" modified="2022-10-12" version="1">MSQTDKELKRIEELEEQVNQALKRSRQLDDSTKFDENFFKQELKEMQERCQQNQQTMIRLEQKYLKTGYELKNSDIIRESSLDKSSVKSKSLLEQKNQQIQELQKKLFDTERQCNQQLSELNRRNQQLEYQNQQLQEDLQNLQNQQENIPDVMELHRALEELQKQYLDLQNKLQETMRNDQQDYQQIKQEYEEYQLLTQNIINQEEEYQRRICELEEELQQSSTFQQEFMSRMKSNEELYQQRYRDLKQKYQQQLDDQRKILDQQIDELREQNNFLFGQLSNAECKIQQLEFEIQRKNNLQTSIHQDNSVDLQVSRSKSFSACHGVQNVKTLTECEINSKVKPQHYVGCYSKGKSSCTQQSQSISNKQDQRNDYKQYDSRPVKQINISIEKENIQTDTLSSYNKIRSQSKEQPITYKQVAEQRSAILYSKIPKPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJB1</accession>
    <name>A0A8S1SJB1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0080108</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="313236at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006816">
        <property type="term" value="P:calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014821">
        <property type="entry name" value="Ins145_P3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013662">
        <property type="entry name" value="RIH_assoc-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015925">
        <property type="entry name" value="Ryanodine_IP3_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45816">
        <property type="entry name" value="MIR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45816:SF4">
        <property type="entry name" value="RYR_IP3R HOMOLOGY ASSOCIATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08709">
        <property type="entry name" value="Ins145_P3_rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08454">
        <property type="entry name" value="RIH_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="2735"/>
            <end position="2754"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="2788"/>
            <end position="2811"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="2878"/>
            <end position="2904"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="2925"/>
            <end position="2951"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="2957"/>
            <end position="2978"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="2999"/>
            <end position="3022"/>
        </location>
    </feature>
    <feature type="domain" description="Inositol 1,4,5-trisphosphate/ryanodine receptor" evidence="8">
        <location>
            <begin position="45"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="RyR/IP3R Homology associated" evidence="7">
        <location>
            <begin position="2289"/>
            <end position="2392"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="6">
        <location>
            <begin position="2862"/>
            <end position="3032"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8139457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="3132" mass="370758" checksum="15D97F06A1AB0B6D" modified="2022-10-12" version="1">MELPPIPILKNQSQFGIDNLVSQTHIYNTQTQNQETTKPFKRNFNLKVGDIVYFKSYLTDDYKGVISGDGIASNKLECIQILGKNQQIIDPSKKSLQQKVGSLTFQKSLFQVITGKKYHYQNFLEQERSRSIDDIREQAINEDIDLDQCQLEYEGRLKELEDKANEEILENQRFYDLAYGSNIVYGQEIQLKHIYSSCILSFNSAILAKENCCRELSLEEISNLNSNFRILSMNQVKNPGEPILYGDQIIVQNSSSNKWFMNIQKPNQKYDKKDGLEVNCSQIGFPLKIACYVDSKTEQEIKQQIIKQKALLSGDVVTIKNRYLGGYLSIQRQLRITEGLDKKEVFIKNYDTNITYTKHQFYFDVEQVRNEKIEYMYKLYVDSSTDAENNLNNLWQIQHVDSLTFSKPNYESVFLIRHVCTGLFLEISHHSANLTYDGLRQECQFHLRSKKTLDDQILFSEAYKLQSVMNVQIDGYVTQENLVIVCLDDRVAVQRKSQKQNIERETFLLKLATPEQAKTAFRINSLQEYLIQFYIFLQDWGMLKTSAQGLEVIRTYDYFEAFNNQKILYDEILQLFQTLENLKLYLNNEGQAQTNEQQQFKQKALMDNDIINLLFAIQRLCNFMIYGSLSNNNSQIQDKTPQKIAKAKLDPPISSSQKLNCIQEIYDVLSLCVQKNQDTSNYILTLKMNKESILDFLLQQLKYQRKYISNLIKESVRYTDMSESKENIKKWVNQLENLSEENIEDQTLYIEILSLIMIDPYENPNKLCQNACRRLLFGTKNQQEQQYPFHKVLVSLDIQEENNNYYPIVQFYPKREKGLQMQYVNEFGQNNQLFCQLYLKFIDKQARIAQRFLNKRPSLIDVVIDFFTIETLKENQLVKQEDAKLVIPIFQKYENYLMNVMGLYSSLCKGRNQKNIKCLMRNCFLNQKFLEICLKRQQNIKSELRFEKTLIELFCNLFLDIDPLIKISQNYDPFYNNITQINTIKCYLSDDIDQFDIQNPSGLYFYENNASKSLKRSDDYQSFLHSKETKAEHQQLKLYAAKMLSRTHINDVRLYYLEMFTKEDYPEHLLQEASYDIFKSKKLSQNNKQKYDYSRLAYLQLHYFLGLLTIIKNSINLGYNQLDENQKIFSILPNIFVALILQQIPDMRIHRFSENESFISNLNKLRQSIDDNIWVVTFLPTNEQLYEKRKSKHKNLDKDYKRMDQSSKLYMQFQRNWILYMLIWTYQYCNDEILRMQVYLEALSILKIFEQLKLNLQILEFLFSSSQQNESNPSSPSFRDYDQFGFDEVLNQEIQIKKSKTPNSKSIYSVFKEPDGNSISGLLFTCLLTSGKRNKLNEELLKRIIQSFHAPKYSIQEINQVEIIDNLQELRYFSIINGNSNSVQLMSPKEAIQLSKRALKFIISQKDNNVNQKRITSFDLLKGYSSKIIEQFELFFKPNSQDFQYLKSFQNILRNANIHLVFLKFLVDPEIINSSNDIVFFYKRIVLFLEYFIIDNDTNIAILADNQYLFKILDVIQIPQPQAEDKFYIPIKITKLAIKMISTIPENQHDNLINKIFLKIYNIGNQLMQSQEFYLKVYCARDENEEIKFIKRSQHENAVAYFSLIQYFKILRSMTKTYEKPAKQAPLNKHLILSKILKDNFLRIVLDPVNYYKDILVPELVTHEEDAKDPLLFHRMKLHSELITLITECCQYYRLGIQEMQRILLYEQLKTILLAPNSEYIVKRAYLQCLFELYINQVKQGQFINDTIESDEVRDILSRVIIPELDQKQICKYLEGLSKLANQDRNIKVIQRDLRDQIKRQKQTYFENKLSFDSKFVKEQGILRDLSEYWKYLRKNGVIHFLIYTYDEIKDRIDLENPEIQNLSDEFAIIKQNFVKIKEIFNVLERDFRVKKEDLDLDDYRQLIISIEEIIPQRKITKFGQNFRIGFQQDKNDNKLIFDKFDTLKDDQTGEKKLQEEDNVQRIENPFRRNFKVYLIRYKVNINQFNEYIESKANEQERLQKILDTCNIYKTYIQQKDIDEIYKMNVEQIKRQEQINNRNNIVSQWDLFKQAMRELFEMQTTQNVINQQVQTYQRQQEGLENLKQQFGIACRKLIGKQYKKHGGQLEGSEDLLSDDIQIELLIKTLEQQNTNLLIEDNVQEFLQKCQEIFLNREVYLIKFCKIFLFLKRPTQDDIDSLTEAKDEKSKDIIRQKQQIFIKYQNAMVDGGLDIIALDILNESVDDQHKIEALQLLIYLLDYGNDYVQKKFYNILKQDQIIKSKFFVFLRGFFLTDINSRILELELCEDNNTEYKILCLKVLKLLQALCENVNVDFQKFLVRQYDDDSYQANINIVNEVASLLADLLEKGQSVFYKLQEIYRQALESLVEFSTGFEENKKELCKNTRLFTLLNQILQKEDLISFKQLQQENRNILKENLLKSQNQEEDLIINLNKNQINYQERKYNSYQTLQSFIKLLLLITQGKLNKKSLKFIIETVNITILMRISRSIYQDRIKPKQRNIILDNICDESKTGQHQYCSNNLCHFGLRTDEDNLLIQTGFNIFIICLKLSEHFPNDPQLEIFKFDEEYDEQDDFTDFSDPLQEELQKKNTVSSQKIVPIFIKNQNTEKEEENALLSNRLRQTSFNEFKQILEEIQNDDNYKFTRKQQFFKFYRQFTGRIEIQNEQSELEKIFFQKPFVCNFVTPNIKQHLIYEIKRDTDEDRMQGLIDQADFYQVQMRHSQQINNSFIMHFGAVYWRLLKDISFILCSVIVILLIFMHDIVVNSKLGSNQEAPESNTKVPGENFVSYLNNIITIIQLVLNLIIVFFCAIERYPISITYNRGETNAKKVQILKKEAGFSIAFLTMKYYSIIGYFEQEFQGQKVSESTIKKLILVIFFDFDNFYNICIFGLTVYAFFNPYIYAVLLLDIIKRSEDLQNIIKSITSNGRNLAIFSFLGFIGLLIYAIIAFSNFSWMFNDEDGVYGQTFILAVTSTINFGLRSGLGDSMKTYPQPYEDPTLYWGRYFFDFSFFIIFNILFIQIIFGIILDTFGELRDERQALVKEIEGKCFVCSLEKNDIDTNGSKGWHYHIYLEHSVYHMLYYIIYIKNKDLNDCNALEKFVKRCIDGKETAFFPFGRALQIEDQNNDEDELIDPIES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJV8</accession>
    <name>A0A8S1SJV8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0080476</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8140148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJV8"/>
    <dbReference type="OrthoDB" id="285490at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8140148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="99" mass="11869" checksum="6AEB6D586DDBF36B" modified="2022-10-12" version="1">MKNLFVPLYRMNVVTPKRYFILQINQPTNLQTQSLFDQGHLIFSAKKDQGMMYIFNTKDEVIPYDFITKDNSIKDFKIEEIELRTQGESKQLSHLYKFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SYP0</accession>
    <name>A0A8S1SYP0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0130259</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SYP0"/>
    <dbReference type="OrthoDB" id="288288at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="487"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="291"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8144498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="587" mass="68349" checksum="0EBF1485B0916768" modified="2022-10-12" version="1">MQQTQFFGKKIKLQQISLTGNYECPPLPTLSNIHGLFSPRLANQKEFLQSPRKKVYTKVSQSSRTDVPLISQSQRNLDGFNITQQFSISPCKSYRSTLRCLITHEINFRKNLDHIYIVKQPVNEINQLKIMQMSRQKVNSGYKEDDIYLKKKKLILEAQIALSATRVHTQLALFDYEQINFFEEIPQNCKLVFLSNKTMMDFWEILFAFHNIELQSDRQNQILNIIQYINSTDDLLKFLQDKQLFNEVIESRDICSPIENLIITTSKSKQQQFQKFNTSNDLQFKLDQSLIKQMQILKTEENEEIQMIRNRLDENLKDYKIEVKSDTIQEILEKQGLNKNIKQNQQKYFKLSNFKTQCSTIAKTQSKNQTYINQFTKFPLYIAQSQPLATYYNIPKLMEKSGLNRNEIHEIYSRYKALLSFECTQIPGMTKQMLPNGISRDSFAAGLNELSMAPSGVIDQLFNLFAIENSLNFEGFLQIINLITAKSNEKKIELILKLIDENGNGLLSFEEISQRCEMMMDSIMKYKNGESCTNNMVDIITKSIFDAVEMNYDEEIPIEKLRLLIESRSQASKALLMMCCGDINYID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SZP6</accession>
    <name>A0A8S1SZP6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Dynein axonemal light chain 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0150358</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the dynein light chain LC1-type family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SZP6"/>
    <dbReference type="OrthoDB" id="266138at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000049">
        <property type="entry name" value="Dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454:SF73">
        <property type="entry name" value="DYNEIN AXONEMAL LIGHT CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454">
        <property type="entry name" value="NISCHARIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0243">Dynein</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8146465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="201" mass="22660" checksum="9B552B397E72B85F" modified="2022-10-12" version="1">MAKTTCAKALQNWEQDHPGEQPSEAEDIRLIFQNPPLDKLDPPVLNTLAKVKRLSLSSNAIEKMVNLPGLRQIEILSLSRNNIKKIAGLEEIGQTLKELWLSYNYIERLDGLQPCVKLHTLYIGNNRIKVWDEVDKLKDLPEIANVLFIGNPIYENQKDDPKLLVLKRINTLKNVDGTVVDDSLMEKVKALGDGVPTTTVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T0L4</accession>
    <name>A0A8S1T0L4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome biogenesis protein NOP53</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0130135</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NOP53 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T0L4"/>
    <dbReference type="OrthoDB" id="5072at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011687">
        <property type="entry name" value="Nop53/GLTSCR2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14211">
        <property type="entry name" value="GLIOMA SUPPRESSOR CANDIDATE REGION GENE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14211:SF7">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN NOP53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07767">
        <property type="entry name" value="Nop53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017302">
        <property type="entry name" value="Gltscr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8144252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="316" mass="37239" checksum="00A5CEDBABA15D40" modified="2022-10-12" version="1">MVNIKKKNLRARQGTQRWRKNIIVEMGEFDNIDFTQNDAKPVDFQVDAQPINIYENKDRFKKKPIDPTKLSKLEKIKIQNMAEQQKKQQQPEKQKEFQDIWAEEGGLKKIQSPKVKAIVTPHPGQSYNPQLKDYNELLDDVVKKEIRSHQRLQNVETEVEKAQKLIQREKEKKLKRKMSMIQKVHGKEVVDRLIEKKKNHGLLLVKNIKKELNAEARMRLEKRKQQRKEKKLLERARKLSGRNLVPIKIGNKKVEVTQDEFVLDDQLKSHLRHVTQGDLLVKSEFDGFVRRGLIEPKSKSNKLSKAKVPLFKIKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T1E9</accession>
    <name>A0A8S1T1E9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RuvB-like helicase</fullName>
            <ecNumber evidence="8">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0100469</name>
        <name evidence="12" type="ORF">PPENT_87.1.T0130434</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the RuvB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8142110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T1E9"/>
    <dbReference type="OrthoDB" id="10060499at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000010">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.360:FF:000001">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027238">
        <property type="entry name" value="RuvB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041048">
        <property type="entry name" value="RuvB-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010339">
        <property type="entry name" value="TIP49_P-loop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11093">
        <property type="entry name" value="RUVB-RELATED REPTIN AND PONTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06068">
        <property type="entry name" value="TIP49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17856">
        <property type="entry name" value="TIP49_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="10">
        <location>
            <begin position="63"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8142110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8144854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="455" mass="50093" checksum="B2BF5A2E985700CD" modified="2022-10-12" version="1">MMKIEEVKSTTKTQRIAHHSHIKGLGLSEDGTALENASGMVGQQIAREAAGIFVDLVKSKKLAGRALLMAGPPGTGKTAIALAVAQELGSKVPFYPMVGSEVYSAEVKKTEILMENFRRAIGLRIKETKEVWEGEVTDLKTEEKDDQTGYGKVVSAVVITLKTSKGSKTLKLDPSIHENLTREKVTIGDVIYIEANSGNVKRVGRCDIYNSEYDLEAEEYVPLPKGDVHKKKEIVQDVTLHDLDVANAKPQGGHDFVSLMNQLNKKKKTDITDKLRGEINKVVSKYIDQGVAELVPGVLFIDECHMLDIEAFTFLNRALESTLAPIVILATNRGQSQIRGTDIVSPHGLPVDLLDRLLIIRTTPYNLEDIIKILAIRAQTEGLKISEEALQDLSQIGNEASLRFAILLLTPANILAQTSGRDEIGKQDIQEVHDLFLHAKQSSKVLEQQADKYIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T1M0</accession>
    <name>A0A8S1T1M0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0130470</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T1M0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8144924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="270" mass="32226" checksum="D19179DD083B7133" modified="2022-10-12" version="1">MVISKNVKLVCFDESCKGERLYCIQCIKNGINVSHPQYQEELPKLFVHIQSIEKECDDVINRMNIQMDLLQQEFYLLFGGISSKYQISKQQFLNLNSQQMNSYLEQSVHFRSFEFKIIQLLQQFKNEFQFQIQKLLHELQLSELSYYQISNLYLTKSEELYQKGYKLRIQDNSKDKKHYEVIIIFDQALSLNSKHQKALLCKADSLRSLCQYDDAIILEIKLYKQIQSIVFHYVLKVLIIFVHQRCIIHIKIPFFQFTKFIKVQRSYGSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T4V1</accession>
    <name>A0A8S1T4V1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0180238</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T4V1"/>
    <dbReference type="OrthoDB" id="293936at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8148781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="408" mass="48583" checksum="C71C202306B4C900" modified="2022-10-12" version="1">MLSSKTLPEQNFGELAESENSNEKSPKTINSSSFLSFEELQSFIMEEIQISYEVIENYGTSKNQFNLLKIINESDVQNQNIYYCIIVNFTDELLKQKIKIDISRYSFETKFFDFTDWQDQKDSLVLSDSTSTTEPQVFKDYVSINSQFCRIIVFSQEFDQKIIDLINNYPYIYYCNDVKIVKEEYLESLKLHQHFQQIDINYKMIKHLISTSLSRLKDIKFVGIQIKGCIEYVDKIDQIKIHPLIDYQRIGKKQIQFKYDIPFIIINSYFYLPIPLSIKNLEIKDYLTQQFQSKPVHISSNINHIEEYDKIYILLNSIQDQIKKDELFFSINSNKEFNSLESLLTLNQVNQIQDLKNQLIDEDHYLNLIEKILIYFSQCLKSTIEKYILFVQLKHLILYRLHNSYLNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T8N2</accession>
    <name>A0A8S1T8N2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transcription elongation factor TFIIS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0180178</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T8N2"/>
    <dbReference type="OrthoDB" id="282152at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003618">
        <property type="entry name" value="TFIIS_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51321">
        <property type="entry name" value="TFIIS_CENTRAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS central" evidence="7">
        <location>
            <begin position="140"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIS-type" evidence="6">
        <location>
            <begin position="272"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="81"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="86"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8148663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="315" mass="36947" checksum="D28BB4AADACFFAF4" modified="2022-10-12" version="1">MDYQELYELRKKLVDWKKNPKETINILKLLEQTPIDQEAVNKSKIYKTLHTLTIIDDKSDPLAATIRTKAQIVQDKLKKLSQNKPNDNDTIEKKREKSEEIKMQKQNSVQNIQSSKSLPDIDLFVNYKVPYPSYVDRVKYLTAISKLFILNIQIFKNQDDLSDSDYQIIKDSVEKMEKAIYYKRQTEHSPRKAYEQDLKILAGFLKKDKNGSLTYRIFSKQFDPLIAAQLKAADWVDDETKQEQSRIIREKMEAEQLGFYKDLSKREMEGVEGKMCKGCGQKKVYLVDEKQTRASDEPTTKFFECYNCGDKFRIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T8Q6</accession>
    <name>A0A8S1T8Q6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0170338</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8148178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T8Q6"/>
    <dbReference type="OrthoDB" id="10411012at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8148178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="117" mass="14118" checksum="F0DF7C917B728455" modified="2022-10-12" version="1">MGNINKVFIQTRRGRTNYGYQKKNKETYGFMNLPNNLKYLFLLNFSKCKIHINRRCGLLHLSAYHGINFYDILEKAIKSKVNTEIKRSLEQQIKRYQKIYNENNWGNELQQKQQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T8Y3</accession>
    <name>A0A8S1T8Y3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0170360</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8148218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T8Y3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8148218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="180" mass="21563" checksum="37F5BD61BA10A575" modified="2022-10-12" version="1">MFLQYITITIRRILKVKNIINLDEFLLDVAITEVSDLQFTEHDYQNNLTKQKGNLDYLGLIQSLVEQMIDLERINLQSIEKEFGELLIEETDPLSLTERIMKNLYYYSIEFSLKIINNENQYQNYLNIEKENYVLLLKQLRKVNCQENCQVVEQLFSKIYQLLYYYQQNQIRQAQVGLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TD68</accession>
    <name>A0A8S1TD68_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0210194</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="193091at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000068">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000061">
        <property type="entry name" value="Sodium leak channel non-selective protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000128">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000117">
        <property type="entry name" value="Voltage-gated Ca2+ channel, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000300">
        <property type="entry name" value="Voltage-gated Ca2+ channel, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000095">
        <property type="entry name" value="Voltage-gated Ca2+ channel, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037:SF62">
        <property type="entry name" value="SODIUM CHANNEL PROTEIN 60E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="120"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="329"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="484"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="527"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="558"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="612"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="653"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="691"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="832"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="960"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1017"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1058"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1143"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1173"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1200"/>
            <end position="1218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1253"/>
            <end position="1286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1363"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="1403"/>
            <end position="1438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="371"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="436"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="381"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="438"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8151145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1568" mass="180000" checksum="68ADD74004CDDE69" modified="2022-10-12" version="1">MSDQEVQNEAQTQIQPQENQKRLVHLSSLVNHMIEQGKDDPGDLVEFSVHGKLLTYTRKSLNLFDAENKIRLWAIWMIEWIWFDRFIITVILLNSILLATQDYSWRENNSEAPSNWTDSLEYLFTAIFIIEFLLKIIAMGFLLDKQTYLRDGWNFIDFIVVITGIISLFISARVSAIRIIRIMRPLRSINSLKEMKVLIISLLDSLPALGNVVIFLLFIIILFGILGLQIFMGALENRCRETEYPIGNVWKASNYSKLCQDSSNCPEGTYCGNPNTYNLPQQELDDENFNYGYTNFNNIFLATFTIFQALTTEGWTKLIFMYQEALSTVIVYIYFLLLIFLGSFFVVNIILAVINSSFIEAQMRSSIKNSQASASSQQLDEHEEELEQNEEEEQQVDPIALTSNINNGDQTQNHQIEQDQQIREQIPQNKNEINTIKNQDNQNNGEQQQISKSNTVEQVQQPRRQSSKQKSLKLNQSKFRQFQLVLIIIIENRYFLAFITSVIILNTITLSLDRYPISKTESDILDIANQVFTVIFTIEMTLKLIAFDTNYLKDSMNIFDAVIVILSLIEWILSSAGNDSSGGSFSAIQAFRTLRLFRLFKLARTWSSFRKLLTAIAATVGGIAYFIILLLLFMSVAALLGMELFAYKTPYRYNFNNYLTSMLVVFMLLTNESWNTIAYTFMYDLESIYPVIYFVIVIIFGNFILLKLFIAILINNFHEANVESSQSIEENHNLHSSRHDQSMAENQQSIKINQISPEQAQQSFQVIKSASNQQTNNNSATRFKNLSQQFMASAIVFNSTLNQSQLEGVSLYIFPPQNKIRIKISKFINHKYFEFFILIIIIISSILLALDDPLSSSNSTTLNVIDEIITALFICEALLKIISKGFIINGPDSYIRSPGNILDLIVIIFSSLAFDQNQAQTFSKIKILRVIRVLRPLRLIVRNEDLKMSINALFNSLSQMMNIALVCLIFFLLFGIFGVTQFKGAYYYCDIQEVDDKLECFDKGGSWSNKNFNFDNVLNAMITLFVISTTEGWINLMSDGIDSRGINLNPKENYAYGWAFYFVFFIIVGSFFIINLFAGVIVEAFQSEKDRLSQMRDLSKQELEWYEIQQKIYQSTPIEKFKISKHKFIRQLNEIISSNKFEIFILVVIILNTCVMMIQYLRSPQELTDAIQILNWIFLAVFSIEAILKLIVYRKFYFTSGWNVFDFTVVLLTILGVILEQSNVLNNVGTATSILRTFRIFRVLRLIKSAKNLRIIFGTFLVTLPGLVSVGGLLGIMLFIFAVVFMNLFPYVKRGEGVTENSNFSSFGVSLFTLFKCSTGEDWNLVMMDTARTAQPNDICFDFNDYANYAEYGFMGCGNVGGILLMIIFMIIVSLIMLNLFVAIIIEGFQKTSKEENAPIKKSDVENFQQQWKDYDKNATGFMQCKYFTQFMIGLPQPLGWSKLKYSAAQQRIRMAQLNLPVYNLNGKNMYFYYQALICAAQDFLESSGFVSNFEISKDIIKLKLQPLMEKAFSQVSNLETEFDSGQYMAAVLIQQNARRSFQRLKKSKSSVVVAQYTNDDEVQQFVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TD93</accession>
    <name>A0A8S1TD93_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0200246</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TD93"/>
    <dbReference type="OrthoDB" id="286378at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035760642" description="GOLD domain-containing protein" evidence="10">
        <location>
            <begin position="18"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="11">
        <location>
            <begin position="27"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="126"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8150410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="207" mass="23945" checksum="B3E53BC76212BA07" modified="2022-10-12" version="1">MQKYFFILVIFATCSNALTFYLTEGKQRCFLDEYTANTVVIGAHQLLDKVQPNGGGVELSVLDPDNQVILTKMTNSEEGKFTFTTKKQGRYQVCLKMSNSKGWFGDKKQPLYFLRIKQGENQEFQSAAKESQITGLKQKIKQLQKQEDDFIKLQQMNRENEDKLSIENIKINDNVFNATLIQIFVIIGAGVFQIYSLRRFFAQKIYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TDD8</accession>
    <name>A0A8S1TDD8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0180323</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TDD8"/>
    <dbReference type="OrthoDB" id="295705at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="378"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="383"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8148949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="415" mass="47714" checksum="89357A9A8A0A5DBA" modified="2022-10-12" version="1">MFKNDKPVIIDDQYLLTSIYYTGRNSVYYNAVLVKDDQKNADILVQIVTNQSQCYCRLYKKTSDNGITPNIHQFGILKVGETSYHYIVQQKAGPSLKLCFLLMKRKFSIQTIALIALKMITHLSKLHSLNIIHRCLKLSNLITTLSSEIYFTHFEYSSKYMDKNGKILIKKKNKLNLDDLESLFYILLHIITLGKFLPSQPNVKRSEKLKYYYEIKQRFVPERELKGFPEQFLQLYHAIRLLSPHEFPNYDILKQPFFQLLGQNDLQLQFDWIHLLKPKNKNSTSPSPVRISKLKHAGSVGDMAFIQQIKKEDSISDTMSPGRQGKPTMKHFAYPTSRNTTTQQLPAIYEGKEGTQHSILQKNEFQKKQGGLSIVIFKQQDTSSSSSGNVSSMSDDNSEIDIKPLNGIIGKLDND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TE03</accession>
    <name>A0A8S1TE03_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Pre-mRNA-splicing factor SYF2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0200354</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SYF2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="408413at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013260">
        <property type="entry name" value="mRNA_splic_SYF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13264">
        <property type="entry name" value="GCIP-INTERACTING PROTEIN P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13264:SF5">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR SYF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08231">
        <property type="entry name" value="SYF2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="511"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="541"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="48"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="177"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="544"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8150580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="761" mass="90571" checksum="BBDB9E1B4EDB18F3" modified="2022-10-12" version="1">MQSTSQRNLLLTSPIKYQTSMKFNQTPNETGKQKQYFIKDKETMFEEIQSLKQSKNQLFINIKKLQAQVQYYKKEAIYKDESSPLKSSSRFKQLYQERIANLKEENSKLHQQLEEIKAFVNSLKNPLIQTNLDIFCLQLQNDNQQLTQLXKQQSQENQEDKKGQLLVEGLLKKSTEEQEQEDDSQEGLEQFEGKNFMTQKHKERYLGLKKKIQQSKVLNDKAVLNEERQNTDAQYDKYVRKRRYLLKKEAEKKELEFKQIDEDKDYLNRLTIKHDEEQERKKEYFGWDVFNEDAVYNAYKKRCTTLAKDEGKYKQQMESNQEFVPTNEALERLSSDITKQQERRKEFSRRRRFNEDQPVTYINERNRIFNKKLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDKEEEQKLQALNPEKFKQQSQENQEDKKGQLLVEGLLKKSTEEQEQEDDSQEGLEQFEGKNFMTQKHKERYLGLKKKIQQSKVLNDKAVLNEERQNTDAQYDKYVRKRRYLLKKEAEKKELEFKQIDEDKDYLNRLTIKHDEEQERKKEYFGWDVFNEDAVYNAYKKRCTTLAKDEGKYKQQMESNQEFVPTNEALERLSSDITKQQERRKEFSRRRRFNEDQPVTYINERNRIFNKKLERFFGDYAADIKANLERGTAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TGT6</accession>
    <name>A0A8S1TGT6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0210130</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TGT6"/>
    <dbReference type="OrthoDB" id="433501at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044640">
        <property type="entry name" value="RU2A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552:SF6">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8151017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="218" mass="25311" checksum="172BA1C65D205A6E" modified="2022-10-12" version="1">MKINSELIQGSHHFINPLNERQLDLRAKKITAIENLGATLDFFDHIDLGDNDIKKLGNLTLLKRLKTLNLSNNRITKLTDISDSLPNIENLILMNNRLTDINEMYQLRNCKKLKRLILHGNLITQQPDYRYKVIAILPNLKVLDFNKVTLAEREKAVESFKPDELTDYMNLVNLKDATIDKNHIKKLLENAKTFDQINQLEMLLKQQQVKNLSILNDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TJ44</accession>
    <name>A0A8S1TJ44_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0250222</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8154141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TJ44"/>
    <dbReference type="OrthoDB" id="298797at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053083">
        <property type="entry name" value="TF_kinase-domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44305:SF2">
        <property type="entry name" value="SI:DKEY-192D15.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44305">
        <property type="entry name" value="SI:DKEY-192D15.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8154141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="405" mass="47933" checksum="86EEB3952A81D084" modified="2022-10-12" version="1">MGNTIVLENEDYLQSNILKDKITYVNSKQHKYLGQIQLWKNKLNPQEWIFSKIDIDCHLIHSQHAQRKQMQHEYLLKYYGCTIKEQTFQGIIKEFRIYYEFLPTNLSMVIKDHQYQGQYVKESFIWKLITQISIVFSYLDSKNKYHSNINFDSLFFDQAHNVKILYHGALPKLLSIYAQTLGNYNSNISLSPQQMKDYQNQSTYSQINPFKQDSYSFGILLVSIMSGKDLTMYTDFYKEVIHYQRILNILGSAFQYYSQSLINIVVSLLQYDDSKRPNISQIFKVKIHKQPILLYQPQQQTLQQPQTINIYRMDKNQSYSTQNHIYNNQNQYLLISPNKKALSITNNTLVYSSPIKTSTRTSYEYPKQFQLVNGFFTPKAKQSRSLSYRFLTSRNISKIQSPYIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TJ67</accession>
    <name>A0A8S1TJ67_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0220062</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8151704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TJ67"/>
    <dbReference type="OrthoDB" id="1305at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="340"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8151704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="508" mass="61030" checksum="B2C1EFFD2727D960" modified="2022-10-12" version="1">MAQFHKGFTWTQCTSTLKTKNTFINLRQTVTPNQGPVLLKIDTERELKYTNDLGWNWHKSWNIPNISVKISLQNEECTQWINCQVQLMAVKMLQNDLFEEVGLEGITQYDIIDGKAFFSGVKFNSTTYNHQGHRFQLLILLKEKENIILGLQSPPVFVDSRKSARDEHRQIQYIQPFEPRYLERNFCKKEKHFNDVVDAPIENNENGLHNYLTAPNIRNKIKHPLFMALKFSRCLNIYYQNNLETDNYLIEFQKQLMSKYQQSQYIIVFQSNNNRIRKKIEESLTQLFGQSMISVVERKYLDETQFKKLEFSSDDYSLIYTQLQELMNQYSQDQYNQSVKQQQEQQQSDMNIEQQQQQQQQEQQQQQQEQQQQQQQQQPIQTEQLLQQQPPKILTHSERKSAFTKYLDKLPTKNFEEEKQEQQQRMEKLNNIREMENYDKVQKIVKVEEELIQNRTQQQQQYSQQQYQQYAPINHPFSILLQQKLQEQILQYYFIQQQLMLLQKPPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TKW5</accession>
    <name>A0A8S1TKW5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0240293</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the argonaute family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TKW5"/>
    <dbReference type="OrthoDB" id="445936at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04658">
        <property type="entry name" value="Piwi_piwi-like_Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02170">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00949">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50821">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAZ" evidence="3">
        <location>
            <begin position="207"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Piwi" evidence="4">
        <location>
            <begin position="461"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU361178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8153575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="764" mass="89342" checksum="0B348F2D4BD5F56D" modified="2022-10-12" version="1">MQILDQQQTNYKPPSKPPRSQNISKYFQLMKFSSNHYEIKFLKNLQLQLYSITFAKELPQEQQNELIIYATGSSQFIRKNGINRFWISGYNLWTFEYVEKSFQVPGCKQLQNINLIIKHAKTYNLTQNLDQIPPESLKQAMNAILSQIYQERNLKQIFRKGQFFDKDKIEKINDVQILQGFTSVLQIGQTSPLLLIDFKTKIFNKQTVLQFIKDNKKSKNPIDLQQFFENKTFMTIYSKRMYRIKSIDLKSNPTFKMESSNITYEQYYYQRYKIKLSDLTQPLLVHEDRKTKQIIRLIPELLYMIELTNTQKNNFKVMSNIKQQTTLDPLERSSKIDNERALLDKYFTNHSISLSNNSVTPAYKIIKPTLFPEANIKQTDGACFNLRGKLIEQSNINNWILIHYNEEQQIANLFTQEFIKSTKELGNQINAPKKIVMNNLDPAQWSQTLLNNFNTNGKPQIVISILDRHEDSKLYFELKRFLISGQGVLHQNVSIQTVDSKKFSKIAQQIVKQVHSKIGKQLWNIQKITEVSDKIMIIGIDVYHKTLINNKSCVGFNAQFGQQSDKHFTKTLIVEKGKELNKGVGQLLNQSLQQYQKSNKYYPDTIIIFRDGVGNSQIDDLIEIELKSMKSVIQQTYNIKLPQFAYILVNKRISDKFFSPNNENNGMIFPERITSKEFDFFLIAQQVNQGTPIPTHYTVIENTTNWNEDTFWRFTYFQCFNYRNWSGPVKIPACLQNAHTVAYRVGEVIQSDAHAALESLLYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TM22</accession>
    <name>A0A8S1TM22_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0220268</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8152079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="415597at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052614">
        <property type="entry name" value="CFAP65"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056344">
        <property type="entry name" value="Ig_CFAP65-like_9th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056305">
        <property type="entry name" value="Ig_CFAP65_10th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058536">
        <property type="entry name" value="Ig_CFAP65_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057470">
        <property type="entry name" value="Ig_CFAP65_7th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000535">
        <property type="entry name" value="MSP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46127">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46127:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24291">
        <property type="entry name" value="Ig_CFAP65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24507">
        <property type="entry name" value="Ig_CFAP65_4th"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25249">
        <property type="entry name" value="Ig_CFAP65_7th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24816">
        <property type="entry name" value="Ig_CFAP65__9th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50202">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MSP" evidence="1">
        <location>
            <begin position="557"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8152079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1530" mass="175405" checksum="E70CF24AC701B83B" modified="2022-10-12" version="1">MKKKQVEHRMILTNIPSALDIGFTAVTSVSNLVFMLENPNDYPVQFHFEFERFKITPDRGQLAPGSKINFTLTYSPQQAEVIVASIIMHIQYEQPRVIKVSGIGKYPFLQLNTKKLNFETLLIGKTVTKEITIKNSSEVTAQFQITKMIDDEFKDNAFSLDYYSGVIPQKSTFLIKVTYQPKILNVSVIRFKVICQGGNELTFECVGSTVEHAVYLSEKSINFGEIKIGNQATKLLTIHNDSDLATSYQFYTDMNNIFSFNKIRGLIQGKSFDRIIVTFTPKHTINYYERVYCVASNQIKFVDLIGTCYDLLIKPIPLLQQHIDNFRRRVIQGKLSEVDFKYMENSYLMKINQQNTSNLQGEWQENPNQTVQYKELMLPPSSDYRLIKFSEDFIDFGYVECYSQSGARELELHNRLNCKLTIFWTIQTHPTINGEKVPVFTVSPETQSANANSKCKFSISFRPTKSSYYYFQYIQFFAIKYNPKLTKKILDSVKNNKGSILNESFGNNANLKLSQTGITNQKNTIDFTSKEMIPTFSGQIGCVGHSFGINSQPYIPIIELRPSNKLYFPPCTIEESVYQTVEFVNKSDTPIYFNFSPDPTRTFRVFPNQGLIFGKSSQMIIVEFVPVENKAYNQTLVCHMNHQSSNQITLQAIGYCSTPSLKLQNDGKVFFPPSFVGVYSRQKFTVHNESRVPMSYSIDVPEKYQTELYIEPSSGQIKPNEVIHLDCQFIPYKKNKEYRIKVPMTATEILADNQNLIGYHIPGSGNLDFPLEQRKPIELSYQFEIFGKGTDGELELNVKQIDFNIIKVNFNTKKYATLINNSDCTFYIELVLRPKSKDKDKIDHQIISLINRSFTLDLQNGIIAANSKLDIGIMFNPIEVCEFDLVLDVIATEKNPKAPKGPNYQNRKIVSQKCKLEIKAKGNYPLLKIADVRNDSISVATLWENFQINQINTELAKDLNEDEQKFLKIEQLTFDQAQQLQKRLRSYDWNFGYLPSKPQVKSRKIVITIQNIGGTDLEWQFKLPSDHQIELEPWADPGEPTEEDTFEKAILEKNIFQIRPKGGVIAPKCFKDIELIYTPCNLDEELKTKGISNESHFLRVVLQILNGKPLVLNLKGTTLAPLEGRLAVKKNSFELPETPVGLLQPVKYPIEIQNVGSSKVSYKTLIQEMDIDGQIIDSQFNVFDIQNPQGSLLPNEKQYLYCLFKPLEQKIYYFELLVEVSDMVKVIQPVKLAIQGRGYANKPKNQIQKQTIEIPRQRSHQSPIGSKVFFSLEEIDFGELLPLKSAHRMIILYNQSTDRKFTFDFGISQFTLSNNRPGLCCGDEFLIEPIQGELEAQAFIELKLTLTAASTPSVYEGECECTISWENKNQQVNTSQISQNSQAITVDKETLFLRIKKKSSLNVELVNSFKQPPPPIHNAMAHPFQQLLGQIITEVLTDSHTDQILRALDQQPITLYQPEQQPKEGEDIQVEDLTIPRDYKDYKTMFLEEEFIELTDLIMENTFFNIIQETTRKECDLLRISKTFVTPANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-06-18" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TNP9</accession>
    <name>A0A8S1TNP9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0230245</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10259630at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030991">
        <property type="term" value="C:intraciliary transport particle A"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035721">
        <property type="term" value="P:intraciliary retrograde transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061512">
        <property type="term" value="P:protein localization to cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:002657">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:003127">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:003249">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056832">
        <property type="entry name" value="ARM_TT21_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056836">
        <property type="entry name" value="ARM_TT21_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056835">
        <property type="entry name" value="ARM_TT21_5th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056834">
        <property type="entry name" value="ARM_TT21_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056833">
        <property type="entry name" value="ARM_TT21_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040364">
        <property type="entry name" value="TTC21A/TTC21B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14699">
        <property type="entry name" value="STI2 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14699:SF0">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 21 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25058">
        <property type="entry name" value="ARM_TT21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25060">
        <property type="entry name" value="ARM_TT21_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25068">
        <property type="entry name" value="ARM_TT21_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25064">
        <property type="entry name" value="ARM_TT21_5th"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25063">
        <property type="entry name" value="ARM_TT21_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25062">
        <property type="entry name" value="ARM_TT21_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Tetratricopeptide repeat protein 21A/21B N-terminal ARM repeat" evidence="6">
        <location>
            <begin position="12"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Tetratricopeptide repeat protein 21A/21B second ARM" evidence="5">
        <location>
            <begin position="269"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="495"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="724"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="758"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="domain" description="Tetratricopeptide repeat protein 21A/21B fourth ARM" evidence="9">
        <location>
            <begin position="762"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="domain" description="Tetratricopeptide repeat protein 21A/21B fifth ARM repeats" evidence="8">
        <location>
            <begin position="984"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="domain" description="Tetratricopeptide repeat protein 21A/21B fifth ARM repeats" evidence="8">
        <location>
            <begin position="1325"/>
            <end position="1439"/>
        </location>
    </feature>
    <feature type="domain" description="Tetratricopeptide repeat protein 21A/21B C-terminal ARM" evidence="7">
        <location>
            <begin position="1465"/>
            <end position="1671"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="263"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="856"/>
            <end position="916"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8152796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1677" mass="192326" checksum="89C32C82DDC69A31" modified="2022-10-12" version="1">MSSDLLIQQSQVFYLLREGLWKSTQVLCHKFYQRTQDPFFQFWRAFCYFKSGSLNEAINELTLIRNKKEFQFATSAALIYYSQQQRGVDREIIETCRQMQSEGRKTPNDRAFQSAIYFYLFVDEGRKAKEVLELLSQGQPISQITLGWYKLLQKEEQINPSDILDYFQSIGQFNQKPIEYLLGLAKASEINKKYPITLDALNELMIVWRDFPFTDVEKLRFSIFIQDWEQFQDLANKILYDDPTNIFGLKAIAFYNLARKGDVRESLEKIEELFNAIQKQEEDNVQLILNCCQLLSRVSGRNQQILQLTMSQIQKTRKIAPLLGDLCLELAQETLMLEDYEKAYGFFQEAAALDEGRMESLAGMIQCKILQGVIDDAEKQLEFVQEVQVSVGRTTEIAFLQALLESKKSEGTDSPIAQQFIEETLKLHLTQSKLLLPGYEFYIKFNPDFTFTIAQMYLRNLSTNLMLAGKELPTSGIGKGTKLLESIARQAPGLTNVQLLLSTGKMALGDPQEALKTINRVLELDPKNEDGYILHALISIKTKQINLAANSLNQAISNNFAIRENPLFMLVKGEVEFRTEDYKNAQITLEAAYELVAGKYKNKTKVKSKIVQFTEKDKCQVFVLLAKVYAINKKETEAKKIMQKAIQEYAGSPHESTIMMANSEIAIESGDIKKAINILKAVQSGQPNFVNSRIILADVYLKYLKDRRNYTKCYAEIIEAEPTAENYKLIGEAFMKINEPQEAVLSYQKAAELNPEDEEITRIIGNALTMTYDYQKAVNYYEAALQKTPGRQDLLVDLGRLYLRMNNIKKAEQILVWEKFVTDDYAAPTLTTLKANAQGFLLIARMVTKLQQATFNQAQNAEKEKIQQQLMAESQKLIEKIRKAFEFAVQTQKDVIEKSKQEAANVNKEKEQLGLIFLEQARYFFYNERNYKATLDCIDDGIKFIPTNESLIQLQAETFYQNGDKISCEQKLKILQKLNPKNDYASMMLSELVLQQDDSEKSIQQFVQTLQEKPNSFGTLSKVIDWYRKQNRLDEIQIIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKHKKMLLKKVNKKLLINYKATLDCIDDGIKFIPTNESLIQLQAETFYQNGDKISCEQKLKILQKLNPKNDYASMMLSELVLQQDDSEKSIQQFVQTLQEKPNSFGTLSKVIDWYRKQNRLDEIQIIIDNCAKATQNQNEPGLCFCRGLYYKYKNLPKEALIQFNYAKKHQQYAEDSLTYMIDLYLNPDQDLYYSITDEQPKPVAVENLKACDQLLKELQNRGSMSRYLVMQAYVLQLSGIPKSIEMASTILAQILKSNKEWVPAMLSLAINKFLSKKQTEGKTILKLLWAKQADTSGWERDELERAWLLHADAFISIQKYDQAEEILRKCLKQNKCCAKAEELMGLIKEKEQSYIDASNSYEKAFKLTNQRNPVMGYRLAFNYLKAKRFVDAINICKLILQINPAFPKLQSEILSKAIQALKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TVF9</accession>
    <name>A0A8S1TVF9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0270152</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TVF9"/>
    <dbReference type="OrthoDB" id="25131at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="110"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8155532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="338" mass="39512" checksum="8ADD893831ECF619" modified="2022-10-12" version="1">MQEPKSQTNTQYKYFSYELLSRLKINQLKCRVLSIDDKKNLVFFSTQKGLSIYQLKNGMLMQLSQINSQKDVSAITTFMTRSLFLTGQYSSSIIVWSYNLMFKGKYLMKLKKHTNNISCLIIYPNPENLIISGSHDETIKFWYLTPSSSFNCYQTIKEQECSVLALSLNQKGDTLISCDDFNLILIMNGSYTSKWQVKQKIQLKGERLTFLSNHSFAIQHRNSEFIFFYGQDYTSGQYVRINKLQIKGWRQYDRGQFFQYFIPSKQILLIKNGYTLNLIRFTLISSSYCECYLEQSIAFQQNSEVGGVYGTMSKDGQFLITYDDESCEIQIRKYVEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TY19</accession>
    <name>A0A8S1TY19_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0320121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8158721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TY19"/>
    <dbReference type="OrthoDB" id="294378at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF179">
        <property type="entry name" value="OS10G0565000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8158721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="308" mass="35637" checksum="FA11F050A7DFA23F" modified="2022-10-12" version="1">MQLKPEIQKIEPTFKQVALIQARFRGIITRKRVQILKQKENINQQQQNSLNEDLICYEQAQQIITERSTVAQYQDDHGAILMSPKIKNSKFQFGSMTGFGRMVMEDGCYLEGMWLDGFLNGEGLYSVPDGTYYRGQWKNSYMNGQGIYFNSTQNIRYEGEWENNLQHGQGVETYADKSVYKGIFKNGLKDGQGQFIFNDGSYYEGQFQQDKFQGLGKFVWDSGKKIYYGEWFNGKKHGFGQLQVKGKYSYEGQFKNGLKNGIGKIIWSDGRKYDGQWKEGKQHGIGTFQNKNGEILNDVWKFGLIQHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U0M0</accession>
    <name>A0A8S1U0M0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0310144</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U0M0"/>
    <dbReference type="OrthoDB" id="310957at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="196"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8158068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="229" mass="27143" checksum="B298DD556C69051A" modified="2022-10-12" version="1">MQINQEFLCFEKNEKIIEELASLNNHQEIIDFCDSQISSIKFSQINSLTNQTHILDDSLELYKATEQSSGSQILSRVDSFVNKFQIEHAYEVYYKQKIKSLKILQRYEEVIECCDQLQILCQENNQEIYCQKEKTQALFKQDKLNEFIQLMGLDKQENKHNMNYYLQMVKNLKDQGLIQEAVACLDYGIIQNDSFQEQFYQEREKLLKNNEKINEAEAYQKQLDSLELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U1A1</accession>
    <name>A0A8S1U1A1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0300022</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000030">
        <property type="protein sequence ID" value="CAD8157179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U1A1"/>
    <dbReference type="OrthoDB" id="284229at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8157179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="286" mass="33616" checksum="CC0170686CDB4FA2" modified="2022-10-12" version="1">MKSIYQDMLNRAYNTLFHPSVQWFRILTPGYNIFRYPAPASEQINHVEHDFKTPYRDSKYYVRHDEPVIERVAKVTFTDPLNETPTEKLVRLKLLTKDQVNNQEAIQAATQQYEQKYGLPLSQKVYSNQVGGGDALQDFFQGENIREVMASFVRQQWEEVNENALEDLNNTNLDDDYHPNLNEKVGFNLQENDPLFRQLIVDLHTVVKNIGEKTKQEGHLTFFRGDPNYWHVLDNSFPVESIRLIQNTLGDEISQLDPKALQKQQNREDYHIEHKVQVPIENKYYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UA05</accession>
    <name>A0A8S1UA05_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2 NT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0350027</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UA05"/>
    <dbReference type="OrthoDB" id="299012at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019448">
        <property type="entry name" value="NT-C2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10358">
        <property type="entry name" value="NT-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51840">
        <property type="entry name" value="C2_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 NT-type" evidence="2">
        <location>
            <begin position="2"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="155"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="253"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8160519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="430" mass="50434" checksum="972A30B791126767" modified="2022-10-12" version="1">MFKHAGTSENKALIEVNIHQLNINISVPNCEVMIVFQRGDRKAQTQPVSLINKCAKIEQILRIPATLYYDVKQKEYQKKMGTLIILIKYEKGMKNAGQIEIDFSSYLNQKKGKIDEISPLAKCPDSNATLSYTINFLNQDQRSKTPEANINNIREDQSKAQIAQLDLQNKHLKEENIRLQEEVDQFRTQNKQQQIQIQQLLKQEALLKQNYSKSNQETPTAQPEDCQKCYEFQLELELLKKQKNNKQLPCDKCINLEQQIEQLKKQYSQGQKKEALDQQVQLLNQANTNLKEQLYELEQQAIKIRKENQDLKNKLNIQNQQLIDCRERIQELENDDEDDKRILEYQKLIEKSNDDLIEIQQKLFSFKTECDQLQKEKNEYEQQLGILSKKYDSLELQLFQQKQRYANAMHAFLSLGDTNAIEVLEQFSGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UAJ8</accession>
    <name>A0A8S1UAJ8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">STEEP1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0360108</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STEEP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UAJ8"/>
    <dbReference type="OrthoDB" id="418131at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090158">
        <property type="term" value="P:endoplasmic reticulum membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029704">
        <property type="entry name" value="STEEP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057965">
        <property type="entry name" value="STEEP1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46355:SF1">
        <property type="entry name" value="STING ER EXIT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46355">
        <property type="entry name" value="UPF0428 PROTEIN CXORF56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25809">
        <property type="entry name" value="STEEP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STEEP1" evidence="2">
        <location>
            <begin position="58"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8161314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="195" mass="22427" checksum="BD3E08FB27530E65" modified="2022-10-12" version="1">MQDEEKILEQLKELDKAQDKNPVTLASLKTLKSAIYNRDVDLQTVVKLRTLNETTLKSENIKIYFCSLCGKKAIGANIGLDTLPTRRSDNSIAINLKQIFIRLFLKQEGIKYIKRSNSVEKQYRWCCEECGVHVAYQCVSYEEGAQLIQGNSDIQLSNKPYLYVLNDAIVLNQQFSKVHSEIAKLKDQMEYEQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UAM8</accession>
    <name>A0A8S1UAM8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">cAMP-dependent protein kinase regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0350174</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UAM8"/>
    <dbReference type="OrthoDB" id="417078at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000039">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012198">
        <property type="entry name" value="cAMP_dep_PK_reg_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000548">
        <property type="entry name" value="PK_regulatory"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="5">
        <location>
            <begin position="92"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="5">
        <location>
            <begin position="216"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8160792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="334" mass="38520" checksum="20E35BEF8F329862" modified="2022-10-12" version="1">MNPQKVKNGPNFMQQVNKKKHVDCALSSNSESSDDEVDEIVAIKKDVQKQRFSVSAEAYGQFNKKENFKAKFIPKTKEQSERIKQRMQAGFMFSALNDKEIEIVVGAMEEKIFNKTEYVIKQGEEGNVLYVVDTGELECFKNYGKGDIFLKTYYPGESFGELALLFQSPRAASIIVKSDKAILWQLDRETFNLIVKEASIKKRQQFETFLENWDLLKEIKDPYDKLKMSDALFEKQYKKGDVILQQGQKSNEIFILEQGKVSVNKNNQKLFELIKFGEYFGDQSIVAGTVESFTYVAEEEIKLMYIPKKSYSSTLKEIEGTLIKNIQTKYGKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UEW5</accession>
    <name>A0A8S1UEW5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0380174</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UEW5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8162657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="157" mass="19105" checksum="2E80B8EA9A894EEE" modified="2022-10-12" version="1">MQLLLPQENEINIQTKIKEINQTNKILQKEENKKVQQHNQNSEKQLKEESTKPQFQQNFQDNNSHKKLFGSLQKEIQQLKIKSQNFTIIKQLLNLKEKQIQCIEYQYNDIYNVVNDVQQNQNIVMASESCFNEYIYRRRYQFYQIFQIQKKSKEQEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UFL1</accession>
    <name>A0A8S1UFL1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">H-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0380280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UFL1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019019">
        <property type="entry name" value="H-type_lectin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09458">
        <property type="entry name" value="H_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="H-type lectin" evidence="1">
        <location>
            <begin position="34"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8162863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="413" mass="47585" checksum="13A3C1220280C578" modified="2022-10-12" version="1">MLILIEGYIIFDQGYNEQFLYTTGFSCSISNTITTTVTFSGTFQVPPQILLIPKSISMKNQSPRTFDYDIISLTTQQFQIKITCPSDVMVKYLFNWYAVNDNRIVILEKKKQTSCLDQSIYTHSHNLSKAKQAFVILQSWGMSALNLDFYIEVYEITSTSVKIKLHNSNSNLLQIGYQIVIGTQEAFVQSSLIASPGGQNVLLTKSIPKLNLGFLFPSLAGFSWSNIGNIRMQFSQQSILENIEYTCETFDVTQMNTIRFNELWVFQEILTPMYVYSYRITKKYDKYISDPIKIMLIELNQEYTNIGDFQVSIPNALKTVIIQIELKFQPGEKLIGTFLKCFDCGSQNVYRIEQNYLCGNNFIRYRLLFKQQSNFDQTLQISIGISEIKIKQIINQSVQQYQIAQLKFVEVNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UH78</accession>
    <name>A0A8S1UH78_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0390035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UH78"/>
    <dbReference type="OrthoDB" id="10249433at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF197">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_38, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8163092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="310" mass="36106" checksum="6C9B610C99DDEB4D" modified="2022-10-12" version="1">MSDQESSHIALIVVFSIFIGIYLFVLFIIHCFGKINNIIFPAPTPKYQESDFQDNLQYASIYNKDGNLVNGKPNDYSQNLEKIRSIPYVYLKNKYWETNLYIIYFHGNAEDMYAAVLFMEQLMKTINANMFVIEYPGYGIYRNVQPTSILIEQDSLALYDEIKNEFKLNDDQIYIFGRSIGTGPSFYLASQRNIKGLISMSAYKSIRYIIDDFCFGCGCILNILCCLPHFFRNLERSQQIKCPIVLIHGEDDALIQSHHSQEIFENLPLNIQQQSYLIVRPRMTHNEYDIEDDIAFPISESFTDLKPKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UHI7</accession>
    <name>A0A8S1UHI7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein transport protein Sec61 subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0400082</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SEC61-beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8163824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UHI7"/>
    <dbReference type="OrthoDB" id="5401193at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005784">
        <property type="term" value="C:Sec61 translocon complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030671">
        <property type="entry name" value="Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016482">
        <property type="entry name" value="SecG/Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13509">
        <property type="entry name" value="SEC61 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03911">
        <property type="entry name" value="Sec61_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="22"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8163824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="83" mass="8731" checksum="DBA91F961DC0CBDC" modified="2022-10-12" version="1">MSEKAQLAKDAIKRRQGGGKSPSKTTENGGQSGLNFYSGDVSSLKVQPNTVLIISLVYLGIVVLLHIFSKLRSGGAEATPEGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1USD5</accession>
    <name>A0A8S1USD5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0460230</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8167103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1USD5"/>
    <dbReference type="OrthoDB" id="288277at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000535">
        <property type="entry name" value="MSP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00635">
        <property type="entry name" value="Motile_Sperm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50202">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="MSP" evidence="2">
        <location>
            <begin position="8"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8167103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="265" mass="30805" checksum="8FEFB381B755D1D9" modified="2022-10-12" version="1">MYDQKPYLILEHPHAQNPVLSFKYKTLSNNDKIWISHIKIKNPSQNNQSLAFKVMSSAAEQIKSYPFVGILKAEESKTIKLIAKETIFDKTVKVKIISMNIDKPQEINDQYEILDQFKILKDQIKDLPSIFLQVSNFIQSETVSQITLPSDRRILMFTSNISQIQNGNSIPVSTFMKSMISQRKQKDSLQMSQNFQMEQNICSLQDLQQVLINETRELNKQIVLLKAKQNIPFEEEKDDQVKFTMFQLFVIALISLLIGFYMPEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1USL5</accession>
    <name>A0A8S1USL5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0420212</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="303543at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332:SF175">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035898026" description="EGF-like domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="2678"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2306"/>
            <end position="2326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2524"/>
            <end position="2543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2549"/>
            <end position="2569"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2581"/>
            <end position="2606"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2618"/>
            <end position="2642"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="466"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="509"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="549"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="645"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="693"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="842"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="890"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="927"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="1026"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="1171"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="1220"/>
            <end position="1256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8165436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2678" mass="307759" checksum="721A43338CCEFDD9" modified="2022-10-12" version="1">MVILTYLYVLLIQFTILCCFGKYIVQSDVTIQTTSSQWNDLISNSQLSFCTNEKSENYIGQATNSNQIQWQKTFNIYPLTSKVYIYLEFQISGEWNDEEVILYVDNVKQYSEKIQFMDTNKLCNDNFLYNSFMYVVYNMPQSTMDLQIRVNKIDAGLYIKNVIITQRTQRGRGEVLQTMSNLHICLNGLSLAHYYCQCNNPLVYQQNQCVASCDQNYLYSLNDNQCYQKQHCQSCVGLNQNLQCDLRYYAYMESDQDLPVCVSKCPNSYYQDETTQTCVDYQQYLSTNQNSGYPVDYVYFIKTIPEFMHYFLLNDFQVIIVSEPIRPKERNFFYMDNNFYIGSFNNFKNQTIQIQTIIKNPQHKVKFRAICHFIDFYPTNFQVYINEEKQDDIPLLLDTQNRIKQDKEDYILNYEWTWFKTKDINLVQNSILFRVEQPLQNGNYIGYFLLEDIHIYSFQCMTGCPSCFTETSCPPCTNPAHKNTQDCTCLTGYALQNDICIDCPTYCTTCLTAGNCVDCIIATQRLNPPNCNSCPDKFYVKSGEDNCQPCQTGCFKCIELANCINCQNGYFRSNTNQCILQCPDGYFNDSNVINDPKCTICDSNCLKCDTTATHCTDCIASGFLVDSYCYICLEGEYFSGTSCSPCINGCQKCISNICQQCLEGYYYMDSKNECQIQCDDGYFGNYQARQCQICESTCKTCDNSTNINCLSCHIGYVLNVKNIISGECKFTCLPGFYKQGDNCYQCWNGCASCIDQTQNCLVCAQNYFRLKSNGLCYGQCPNGYYNNLIGYLCSPCHPICRTCSGLLPQNCLSCNSPLAYYQNECLTECLDGYGNVNNVCTPCVSNCKKCFGTLQNQCLACVQGYYYLNNQCYVKCPRFYNSIRPQYICKCQFINCIQCLENQYYFNNLCYDECPPGYFGFNKLCNLCDVTCKTCFGEDIIDCLSCNNNLILWKNKCIPTCLGNTYHDILSNECKYCNNECVACNGPNNNNCLVCPQNKLLTIEGYCADECPSDQYAVLPENKCYHCHSSCLTCFGSSYQNCLTCPKYFYLFECVDECPEGFKISKNQQSCEPNFEIVLDHCTYQCDTCWLIPRFCKTCAANRAPNPPNCDCETGYFDDGINSVCQKCDNKCSTCKGLPNNCIKCKGDRQYPQCSCPDYYYDDGVSVNCIRCQDNCQNCDSDGCTICLADRINLPDCICKDGYYDSYAYYCQQCAQKCDTCEGTANLCNKCSSIRVDPPICKCPLGYFENSDIQCQACDPTCIDCNQYGCLSCFGNRIGPIFGECKCDQKGISRYSIGSVYCTDCFVGVPYFALNEEFTGFNFDFGGAVVYIPFEIDSLCEAFFHPETLIKLGTNPRCDLEFNVYFGLNPNIRVGDLIKLNKEFLLSGCQIKFVQIIPMPLSIPITIDKTTHKPNLKFKDLTNSNICESIKIEFDSLAFNGRQPFDILSWNLILPSPPLPEIEIILQNHTLNHLPYLEFPPKLFESGTIYKFSVMVESFALLQNTIEVQFQAMQRSSINFVSTSNINQFNRFEKIIIPNVLTYINCVEKNPPPQIVYYEIYLNNLKLLIVNGNLTENLTDGYQYDIPITFQPYFLNFNNPFILTYNTILTRSDYIVKSSSEFEFYIVPSNPIITIAGGNRMIGFQDALYLNTTITDKDLNDQEIKSQVYECSWVCLDIIDATYCQNQQKLPLEFNSSCSQYIKPKTFYPYTVSNFEVSIIKQGNRFSTTVSIQFIEIDLPSLIVYGADSEQLYNYYNELIFTVQYPGVNPDLLMYAGAVIYDQYVVATFEFFYLQFKYKQQNYFIDLLQDNTIKLRLSVYDPRFILPSINTQILKMNIPPQNCTLSYQLPESYVEFLDEMNISLIGCKDDNAPLMYSVYLFPNQSTYQQDLITSKFHNFVRLKNFQSSNHFKLILPYYFEDEPFLVFNIKDSKYGIVNLTLTVNVKEFKKIDESIFKDYQSTLLSFDEQLVGYVLITERLKQLKSLSQYKEKLYEDLIKIDVNQTLLANQTESLYRSISNLISQNVTLISINETILLYQINYRIIKAISILSQYFNLQKQNQLTSEQNLDKNEYIRQYYVFVDILSSGINYKVNQNVTSDYLLYLLNQIQIHLQSISVVNEPLYKMIQNSVNLDFGYVTKKQAEFIIGLNSSLGQPIGDSQEVNDIYTSATNYYKFSITRYKDNPFYPTSQFPKNSSGYQAVDPKLQETGSTQNTMLGQDIQFSIPKVRTLKSNTSLECISQLQNGTWSADICKTIKKDMEVICKCEKLSPTSVMESIDQILDKASQVFSLTTLEAFATFPFYQTIIFYFYVTITVLYLSIVYWGIKVDNQMFSQIHAQQEQAKNETEVKNTVQNNLINKQVDSKYTKIGQLRDSSIEYQHTMPLFKRNMFGTGLSYGAIILNRFKPINPQINNKQSILETNPEEKSSKDLNKIDQLFSQSQVLQSTRIIKQGLFNKQEESIKQEDSSNDQKLQRKLTIQDIGLKSMLIKNLITNFRAFIEFLKYFHQILQIVYKDDPIKPRGLRASIVYVSFLGGMAIIFVFQQPNNLNFTIGLSILTAPANKIYQIILEKSLSNKSRMIRMIGVLIMIGGAALISYIMLAGLVIMNQTQQSNQMSLIFIGAFLVDNLIYSPLSLLITYFIHMDIIRIPFLQGILLKILDEKTKEFLFGLVDNIRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UW39</accession>
    <name>A0A8S1UW39_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Non-specific serine/threonine protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0490229</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UW39"/>
    <dbReference type="OrthoDB" id="311852at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000956">
        <property type="entry name" value="CAMK family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001772">
        <property type="entry name" value="KA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF82">
        <property type="entry name" value="KP78A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02149">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50032">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="14"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="KA1" evidence="8">
        <location>
            <begin position="482"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8168664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="537" mass="61847" checksum="BCB550D7186879EC" modified="2022-10-12" version="1">MQSQGQAPLQIEHYIIGKTLGVGAFGKVKLAKHNITNTQVAIKIINKKKMKNSRMGAKIRREIRLLRYFNHPNVIKLYEVLDTPGDIFVVMEYAERGELFDLIAQRGKLPEGEARNFFLQILSGVEYCHNNLVAHRDLKPENILITHNYIVKIADFGLSNLMKDGKYLKTSCGSPNYAAPEVISGKTYCGSDADVWSCGVILFALLAGYLPFDEETTQALFKKIKTADYTIPNSFSPQVRDLINRMLTPDPLKRIKFHEIHLHPYMRSNQVPFYLQIPFKLDEGRRQINDEVFDKLMQLPSVNVKGISHNQIQKSIRKREDKSFVVIYDLLLGQMGIESSTPMTLHNLTKQDLIFNPQIPQVEGQSFDNVLLTEIQKPAPYDYGKELPKDIMAIVYPYQARQIVNAIYTCLEKFNTVIKIKSPDYKLKCYHKNLIKMTKYNSSVELFNEFQKEGDEAGSKNDLASLNFKEENNNKKPKTKENKYSPKEIIFNIQIYKMPSNNNDHMIDFQLCRGHPVVFMDFCNKVIALLNQHFNQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UX12</accession>
    <name>A0A8S1UX12_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0480187</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000048">
        <property type="protein sequence ID" value="CAD8168099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UX12"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="96"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8168099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="519" mass="61500" checksum="3CBF131723938D74" modified="2022-10-12" version="1">MGLCNGKTKSSKKNKSQSQSDKIQNTDPQQQTTSDSQFQRNKRYGHLLQPNSAQHKLTMILESYQMQKEKLISSPISIRSHQTLLISKRNAELNKLLDNDFIPQEEMNQAEKCFYKYFVNKFTGRIYYTNNSDQGEENNHYSNIKLNRAALCHPHIIEILEIENEESQQKDKLFFEYCSKESLQEFIDKNQDLNLEQIQIIASQIISVVGYLHSQKLYHNNLDMQSFHFFVNSKYHYLKLTNINGIFKQKKSENISTAFDLQDRNKSSEQDVWQVGLIIYSMIAKFNATKINNYTDLDNEQWLETIKQNLRLPQNIMGINLRSLLLAMLQLIPQQRIKFNQVKENKLVQLGNQEFLKLHRIKLIPQNLHKKINYLQKCFTFYLIQSMAPHQYLIMKKLYDILDKNEDNNLNLITLCKLLQDLFQDQNASSDYQQMIESNQQITFQDFLVMNSNLNKVITLDNLRKGFQILSNKSQYISKKSIVRLMQTDQQSLNKEFEKHCLKWRFTYDHFINFMEKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UY84</accession>
    <name>A0A8S1UY84_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0500197</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8169173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UY84"/>
    <dbReference type="OrthoDB" id="285799at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8169173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="131" mass="15621" checksum="3D49F5A677A888A4" modified="2022-10-12" version="1">MDQFQTSEGKLLYITYNLYKSNRIGIEQKNEIKAKDFRIQQVMQEIKNETNIQKIERMLENINPEEHSPRDIDDNLSHTYKQKRPKLFKFQKSNFSKFIEAKANEDTDTFSSSNKQLEFRQRLYSLAADSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UZX0</accession>
    <name>A0A8S1UZX0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Calpain catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0520230</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000052">
        <property type="protein sequence ID" value="CAD8170251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UZX0"/>
    <dbReference type="OrthoDB" id="290473at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF179">
        <property type="entry name" value="OS10G0565000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calpain catalytic" evidence="3">
        <location>
            <begin position="310"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8170251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="980" mass="115852" checksum="40D5228011CDE050" modified="2022-10-12" version="1">MKNKNKKLSAQDGKFIYEGDIITDDKSKQVLYDGPGYLFNNETKCFFKGTWKRGEKVCGIETFPNGDVYEGQFKDGVFNGRGLLYNNVQLYPGKKGQYLYDGQFEQGQKHGFGKETYITGGRYEGSFKYNQKHGNGKLVFSDGSYYVGDFQRGLPNGFGVQVAYSKERIIAVFPPPYNNEGRMDSQQNEEIQDHEQEKEKREYLYEGDFADGYKHGIGRLYYPNGSYIYAYWEYNKPARDFIEYIAEGHKWRILNVKQLQTEQSINFFKIIRDNRPEWIEKYPIKGNSYPPAELDGYKVLIDEKGLKREDFEDPEFDYTEQSFSIKDENDQPVGAPIEFERIYTSKYFKKKEFKLFTKEIQPGYLKYSEQYCCQQLTVVLNALTFHSYLLSKVFSRKYEAEIGFYFIKLFLRNEWKEFIVDDRIPVFKSDKMPIFSNSQEPELAWTILFKAFAKYNQSFQYFSNDKIMRAVEYLTLLTGMPTVQLSVPNIFGDNQEYSDQQKKIWRTMAQKYQNTKVRICYLYKDMEDRLKLPRNTAYQIVDMQESSLGKRDESYYLLILASNQKLALKQEITNQISLARQKLQERNPQVRGNDCYFFMEYKDFIKNFQYVYVLCASRYPIQNQQLIEPEDKDKYNHELRFFCKFRTNLAQDCLICITQDHQEKQDEYVIQDKTFPVRIVLAKQKDLFTNKNQVKKKIVSRWQEQANRSKQEREKDKKEETIKGDMKYGSIFEQNSDKKQLNPYKYCFGRGQSSEKMLFKDVSLESGDYVLFVQVETGDNKEQFDLKKMKFWITIHSQKLIQIQRLDQQIQGFQKQVYESAIAIADKHSQDTQKIKYFHKGDLSEQENGSSDLVITQYFIKKEGTYLQHLHNKSTNKIWRQKLYFKLDNMQIIDHRESQKLDICLSKKQTALIIIQQVGAKKYNLEGGEGICIVDLLHDEHADEEQEIKNQDVGSFQEDTQQIFQQNSSKPKFGSLLFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V549</accession>
    <name>A0A8S1V549_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0570099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V549"/>
    <dbReference type="OrthoDB" id="286814at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20557">
        <property type="entry name" value="CYCLIN_ScPCL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF108">
        <property type="entry name" value="PROTEIN CNPPD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin N-terminal" evidence="1">
        <location>
            <begin position="72"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8172450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="170" mass="20166" checksum="F80D36D405B851A3" modified="2022-10-12" version="1">MFEKKEKERINNNLKNQMFTPPNFAAHSALHKITKIMARIFQLYDKLKIESTIEIPKSNQKLSKTLKNLYVMNLNDETLILAFYFVDQITKKLRIFLNLENSTGLLIGSIIIADKILNDQPRNIEKYKLASGLKKEQLIQIETYFLETLDYNLFVSEENFQKYYKRVQDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VGA5</accession>
    <name>A0A8S1VGA5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FPL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0620032</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8174859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VGA5"/>
    <dbReference type="OrthoDB" id="294052at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901096">
        <property type="term" value="P:regulation of autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039272">
        <property type="entry name" value="CLEC16A/TT9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019155">
        <property type="entry name" value="CLEC16A/TT9_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21481">
        <property type="entry name" value="PROTEIN CLEC16A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21481:SF0">
        <property type="entry name" value="PROTEIN CLEC16A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09758">
        <property type="entry name" value="FPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0072">Autophagy</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FPL" evidence="3">
        <location>
            <begin position="27"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="435"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8174859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="653" mass="78535" checksum="18F79F71CF5E0A6A" modified="2022-10-12" version="1">MQVLQQVKKLHKDLKKPEKNHKYRQIIEELSEYFLFGDHQQEELFDYFAEHNILHLFYQKLKSANHQLTIFIIERMSMIITNLQNPLNLNYVLSNPVLHEFINYNYDFNNPEIVDYYVNFLKTIAIRINRDNFYLYFNQRYCTFPLLWQAQKFINYPDQLVKNTIQNIVLSLSKLSTEPKSEKQTQSVIFNQTKIMIQFKHYLTSSPFIQVYIKYIIQIQKLIESLNIDSQDELDKLEDILMFFNDLITECPFLSILLEKLILDQLIQPILDCLLLNKNTSMRVSYEVGLLTIYLFLTKLPLINTIMSYFCNDQIYIDDTIQHQKLQKQSQLWVYDTKNMEQEFQNIFQKEFDDIEERNYQFIQHKQNNEIENPYKNQFLELLRSKHNTLLYLYLSIWFVAKQNNYQIPSLQIIKLLQLKEEIVFSKQLIELIILLMINEDLKELEQLYEQLQNKIISMISNLKETNKKLSEGLCANWDIIENFDWDKFKESQHKIKLDDLKPYVDNSKLSEFKYIYLWILVKCLVKNFQKQRVENPYKINQEIELNQNCEFIQIMNSQNYLILDIESGYLIHIFTYQNQEFGIVKFVQPLKAIECSFINDTLILESNQLCLNSFKPYHIKYQKDVIPSVIDRIYQAQQSMSLLIINKLEALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VI25</accession>
    <name>A0A8S1VI25_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">H-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0640121</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8176005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VI25"/>
    <dbReference type="OrthoDB" id="308347at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019019">
        <property type="entry name" value="H-type_lectin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045329">
        <property type="entry name" value="LZTS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19354:SF2">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0290503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19354">
        <property type="entry name" value="ZIPPER PUTATIVE TUMOR SUPPRESSOR 2 HOMOLOG-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09458">
        <property type="entry name" value="H_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035858951" description="H-type lectin domain-containing protein" evidence="5">
        <location>
            <begin position="21"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="domain" description="H-type lectin" evidence="6">
        <location>
            <begin position="37"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="369"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="614"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8176005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="746" mass="86874" checksum="0A398BFC0EB138CB" modified="2022-10-12" version="1">MKMGLLILMTLFICINGIQQSNGMLDLQNLKAHQLFTKNVQFDQEFDEIPKVVVSIKQIIGDQYELYTRTSNINLKGFDLQIISSSNIDNIKINYLAINDNSLSIVCENYQTKKDQIKVPINIEHSQVVAFITGLKLSKNENVYFKLSSITDSQVIFQTQQKAMGLCLVFGDEDKIAHNQSIITTTLKDNNLEFNVENIEDSITLPLDNQILSFDDIVSKLSVESNEEEQSIKVQNEEEQYLDIDSIQIETVGLNNIQSNFEDEVIQSVDIEYLQPQKQSVSDQLLKIEENHIERVQEIIGQAVDEEMKLEEQEEKKLLTQMNQVNVVDIQNIEDTIQQEIIIQSAYQVVPQTVDELDMDVLYKEFTLSQELNDDLIEYNKNIISQQQQQQQQQQQQQQQQQQQIQQQQQSSQDTLEVIESVDIVQDQLVNEIQSEIIIDKTQTQEQIQKTEQKQQQIDVPLFDEDLSFDEIDVELDLKKEETEQQIAPAQYIIDVYKQFANEDAEKQNQKESEDHPYVEVNTETQTEEMSDELENELDGLRDGLEGVDFSLFFDEFSQPNEIDISRQPVQNQEEQQNKKIQQKYEEPQQFKQPKEKIEDVMKDLQITTDITTTEDEKKLITQLKQKSAELKKKLQEQYDAVPKLEQAKKQTEVSIHPIDDLYPKIESDSKINYEVEGKYVEWSMSQDQKIKQIGLDLTEDDLRNPRFSLLQMDQKSELDIQKQINEFLGFDDLSFVMIKQNLRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VJX6</accession>
    <name>A0A8S1VJX6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0640133</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8176029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VJX6"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00106">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF36">
        <property type="entry name" value="KINESIN HEAVY CHAIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 6 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="10">
        <location>
            <begin position="80"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="603"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="227"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="372"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="147"/>
            <end position="154"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8176029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="631" mass="73016" checksum="9C0AE6DBDD14A3C7" modified="2022-10-12" version="1">MSYNSKTIDLILKVQEPNQKQFTSFQQFFKTNNPPPLLFEKNITPKNVKAQRSFSNKTQDSIKETKRKSFSQLKYPEYRMLKIYARIKPGECQLNYDKSSILIDTNSYNFDRVFEQQATQGDIFDVVAKQTVDDFLNNINGCLIAYGQTGSGKTFTMFGESNNPGIVLRTFTHLFLRNQETIYVSILEIYKDHIYDLVSGVQDLKMKEDLQLGFYVDGLKRIKVDKLENCQELLQIAEENRHVAETKLNALSSRSHLILTIQMGRAKLYLVDLAGSEKVNKTGAIGETLQEAKKINYSLSCLGHVIQCLSQGQDHIPYRDSKLTKLLMDSLQADCRTSIIVAISPENKNQDETISSLKFAQRARFIKKEIRIATQKKTYRDLELEISQLKQELLETKQRMSQTSSVSQFQCRYITDSEIPCSESLRLTYRSLNTENKENCVPNLKIQENSAQLELDKKINQIQIELQGLNKEQNFDNVKRQVAKINELTIEMKKTYKILVSTQGLNNNHLQIQCQFIETQQEQNKQLQKIAEQISNMDSSKPSFNIAINNMIKNLQKQQQQNIDDMQCKLEKMYYDIKKTATAQDYNLNHLALKLVASPNRQQQYQQQQQIKRSKSKPNMNPSVKYLKNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VLY6</accession>
    <name>A0A8S1VLY6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0690055</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VLY6"/>
    <dbReference type="OrthoDB" id="301835at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="215"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="559"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="603"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="215"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8178230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1036" mass="122548" checksum="DAA86FDD0C348A89" modified="2022-10-12" version="1">MLLTIKYQNRVKQLNLDRRINVQSLQILLGQLFNIKNKIIGLIDLDGNYFDLTQFVQQLNQTRCNNYTLVCEDGQNMIDNKIQHTSFKYSTISDYNNKLHFIDFIYDIQEFQNILGDDQYTCIYMIDEKDISSFDFLISLEPLINNLNSWINFYYCFNNRLNDQNREYDIQSKTLWVYKGSKRIVSILLNNDKKMKQMDELLQKLVQTKIQGPSVKNSNIRNQSQLSSQQSPEKRRSKSIRKLVEESQIQSNQGSYNQKLIGSRYITRVQAQVIQREASRGSDIPEDQVSYNLSNPNESIINQQDLLFRIVAELEDKELVDLTMARLVKRLLIEENKEIIGVLLQYSQRTIDIQGLCEKLNNIIENSTNQQRPTSPFQNLKQTKQPIINNNQESMDDIKNLIIERNNYEFTSEQYGIINHLWNEKDQVIIRLCTDIYNKEIKKQQVSLKPLQLYADTKFYELLNQNFKKSEITIIQEYKNSKSGSIYATLQHFRYETNINYFINDLKKALQQIHNQNQHLTEPEKQTNQLSKEINLFKFQSPKAPSPFCVLMPNFQNLANETLSPPPQSQLQQQQDENNNKYQSYGKISDELKRVQLFQNFPTQKQEQQKQQEEIQIDQQNELKQEVSSQKKQQNLAAKDLIFSEENFIQRKKEQRILYNNNIEKFYSIQIDKEINQNFQDMISQMDLDESKLKQVQQLFQDHNEQLYEIIKGFQSSRILMNTRSKLIKLLSEKQNDVQDYRKTKMYNLFMNQVRQFTLQKHLQENEKVYLLKMFKENDLQVLGTLETYLQNQDAQDMLENLQMIIKQYSKFTNINQLGSDLSPLEQPQLNTPVLGAKEILSQIKQYFSAEEKNRLETIANGQGETYIIELHSQFLQDSDLNGFIASIQKLAQQDSFKKIHQSITFEDLLKKLQKDGLLKIDDYMFELLSKKKNEQRIKGVFEIYLYSKNVEDFVESIKSILRLLQQQLIQEQLEQFEKENLLDENKLLKLKEIAHDYHKDYSKLLGAFSLYFEQSQTDPEEAKQEILETLNIFAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VPF2</accession>
    <name>A0A8S1VPF2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0690138</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VPF2"/>
    <dbReference type="OrthoDB" id="47276at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052993">
        <property type="entry name" value="CFA-57"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32215">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32215:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="373"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="665"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="880"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="1165"/>
            <end position="1243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8178333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1258" mass="147462" checksum="E8D69F3EAEF43B11" modified="2022-10-12" version="1">MSQTENKQPKPLKEVLGLKYVFGFRGDIKNSILQGTQTNVQEKSTKTKFIYPAANNIVIFDPIADKNKSQEIIQTVLGSKGVTCLTISLSRRYIAWSEESDAGIIVILDLNTRKTKILSTTDCKSRYYVSLDFSRTEESKYLVALSAPPEQMLIHWAWDKSKCLGSTIINAKGDSIKFHQVFYHPKEDDFVCVMGNGAIKPYKLIPDNPPKPKDSPFQKKKEETHSTNFLSYCILSDSKTMVVGTDRGEVLYFNENCEFKLVLSPLQNSNQQSIEGFPIECIVKYSNGFIVGGSDCMIYIYRKHEGDLKNPYVRIDKRIQNKQIMAKITSLCLTNQEDNLIFGVESGQVFSIPFSADREVANEDEIKFDHLIAPFHSGPITGLDVCTRKSLVVTVSDDRSIRIWNFNELILEVMKECEDTPLAVAIHPSGFHLIVSFSDKIVLYNLFEKDLNSFKEIHIKNCQQIKFAHGGHLFAVANQNLVQIYQFYTGENPQNYIFKQSTGNISTIEWDIDDLGFYTGSDNGFVLYWRLEDNQNKLQLAMIQSQPIECICTPKTYANIELQKVYIAGPYDGQYCIYESQHHTKIHKEATEGTKKEIPLYPIQTGCRVSKMAIFNSEKIMLFATCQLANTSEFTQKQNIKNMKFEPGGLRFAKYPLAPEEIIEIQPHIKGITQMKVSYDDSYVFTASYDNSLIIYDVKDQSSKIEFKDGGQAYGVQAYADEFLMQRDQYKKKIAKIEDLKQKIKEHDINQKIKQNMYTKEKDELIRKLEEQIDQLEKREKAKIQELENAINKLDSDFKEEKKQLYENHEKNKKTTENDFKQKMALESQRLEELTKEKQLKLQEFQRYIKEQKDKSTETKNEKKREYEKQLKKEQELYEQLQRDKEENQRRFIEERNRLEDDAEKEIDKKKEINEAEMKKLSDELDKAELEKRTKKTEFDNVQSTLEQANSKVKEIMEDIAQNQELNRQYQKEKESHDKEIKERDKTIQDKQKRIYELKKKTQELEKFKFVLDYKIKELKRDIGPREEEITKMKEQITNMNTEILHFKKVNANLGLIVTDLNLRQAGMKQEIENQQQVIESNSQYIKAFEYDISETHAHINDFKRLKSDMLKLFNKYVQSVVSKKKFENVDVQKEFIKERAHLETTNKGLKEKFSKKLRVHKQDNNRIMSQNVDLISEINDLRREIKLLQDEEDLKCRQIEQMNDQPEERELDQMIEEQEIEINQYREKLQFLKEAVQQRRLQQNINSDSDHQENEQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VQX9</accession>
    <name>A0A8S1VQX9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0680234</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000068">
        <property type="protein sequence ID" value="CAD8178039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VQX9"/>
    <dbReference type="OrthoDB" id="307506at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782:SF4">
        <property type="entry name" value="TONALLI, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782">
        <property type="entry name" value="ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8178039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="718" mass="86010" checksum="2F97599984E8670B" modified="2022-10-12" version="1">MNKGMSYPMKIKYDTLSLLNLSKVRYKNPALTIYCQHYKFCFEMQEALQEIEKKKYYQCPYCSSQAKCQTDLVQDWRVKAYQEFNELVEDVTIIKGIMVNKYTGNKTRYQNFFMIDECSNQLLKMFESASQDNSINFLIQKDLEKQNLNQQGKINFWNFCLLDRVKINIPVRIFGCKHYECYELISLLLYWEQNRQKKEYFECNHAGCSSRLRITNNAKIQTSRQIIQTQNQDSKYIEEKERILDLQTLFSGICIDLDLLNAINKSNPSSFKFYYNKQTKKIEEDINQVNDQLNDPFIFKFYQQNKDLQNKVSFQVFQKLIFQQAMPISQGDLLQEDKKLGKLVALNKYRNYKVKMLDQYTQLIIEYPVRCKFCKDLTVCMDMRSYIADFIYQKTMNPFKYYTCPLCKTQQTGQMFKVLIQNYIYLDQNMLSYMFKDMSYTKGTNIFEYKGEQYMLQEFMDRQKITRETYIASLTERQVLFKQLFCGVKQDLRIKQPLIIQTCPQKNIVDFKSFYEKLKEINFDLENEELILCKCSSCYSKPIKSFIGNIYFHEAFHEALNKFYLHKESEQDIEFTYKFTDNEMDSMVVRKTIPTKNPKITLVQNNDAIRDTSNLRNLYGDEGFLDLMNDEEYQKFFLKRDIESYYYKAIRMTQNIGEIIIQYQQEGIHANVKEFVENFNRNANQDLKKFKFNLSSIQILLRDDVLNFGQRTFSYQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VU35</accession>
    <name>A0A8S1VU35_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0720151</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000072">
        <property type="protein sequence ID" value="CAD8179655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VU35"/>
    <dbReference type="OrthoDB" id="354826at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05578">
        <property type="entry name" value="STKc_Yank1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000454">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000854">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF374">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="24"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="11">
        <location>
            <begin position="283"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="302"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8179655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="386" mass="44415" checksum="90014E7D04C48255" modified="2022-10-12" version="1">MGNCNINEKKEDLMENNVMSVQNFQFIDAIGRGGFGKVWKVRQKKNKQFYALKVMSKPKIISKKSVQSVMNEKALLCGLKHNFLINMQYSFQDREYLYLVMDLLSGGDLRYHIGRHRKFNEEQTKFFSACIIVALEYLHQQGILHRDLKPENLVFDSNGYLRLTDLGIARIWKPENSQDTSGTPGYMAPEVMCRHNHGVAVDYFALGVIVYECMLGRRPYLGRSRQEIREQMLAKQAAIKRQEVPPGWSLEAADFTNKLLQRKPQNRLGNNGPDEVKEHPWFKDFSWEKLISKEMIAPFIPNGNEDNYLPSDSRRDSDDTMNEDQQIMLRRNSIQNLFNGYDFDNNASVPSSQIAISSTSSSRVTKQPTTTNTPKSAKLVVQKLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VV57</accession>
    <name>A0A8S1VV57_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0730173</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000073">
        <property type="protein sequence ID" value="CAD8180133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VV57"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8180133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="248" mass="29871" checksum="3513F48DFA273226" modified="2022-10-12" version="1">MIQQEQFTDEQIQQYDQLADCTFLIIFSRAQSVQNLLLIYFIYNSNKFYLSLLKIYWNLIVTIMISKRVDLISKEEKVFYYIKLIKLWRLLIEQKMLNNLMNYVKKLNQYQKQEMTNQSSIKDSRIFLQWFQEEILSFSKKRHRYGNKLWMIGKSIGLTSKPTVKGKLGGWLLEKKKEQLLNQVQVNISYLYDLKGNLTSKAVDFAASVRDQVIERLKIYGLNKRMEKNDKFHQYMLYLKLLYLSIYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VZI1</accession>
    <name>A0A8S1VZI1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0790096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000079">
        <property type="protein sequence ID" value="CAD8182730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VZI1"/>
    <dbReference type="OrthoDB" id="307753at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8182730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="155" mass="18644" checksum="2B99F06D927E0B07" modified="2022-10-12" version="1">MKTECLYSSNHNSLMKNQLLKKRRQTFTQYTTSSETKEEFLNRQKDYDKLYKYSYNVEQIPKNIKQKHHSNGANEITQQIIEKRENYGKYFLYKDHQLEHQQRSIEIIAELLRKQKKLITDKQIPIENKGKQYNLIMILVIVFPILCILTVMAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W4U3</accession>
    <name>A0A8S1W4U3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0800173</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W4U3"/>
    <dbReference type="OrthoDB" id="305854at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8183285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="505" mass="60530" checksum="02AB9D55CE4F5DA2" modified="2022-10-12" version="1">MIQDSFQIEDLTKCQIEQVETLMKNLDKIKSENFVFFPNSLIIEKQEKQYEFKFTEENASLIGQRNQKLLQDQRQPIHNSKHILLLLNRLSKLNIYLPTLTQESFLMIEEEKQESEKVGQKKQNYVLKGLLYKFSLEKLHQNTPEIKNNHCISPELINSIQHSQKDGNNIIFTEKMAIFNIGCILFEFFTLQKLYQRESSFQDNCKYALTSYIFLSGFKYDSFLFGQLVQKCTLEEETELNRRFTFQEALDQIIYIEQCVKYIDKAKLEQKSISIHDFFSQKSSLYDKLVESLQMKVNHQVTGRLYILQNLMRMKFIYIFFQEEELECFRDFLILKLIIQELQELKKFDLITNQPLNKQNDILNFKDSLDEIKEFQTHYSSKYQQILFQLIKGIDEHKYNEISQTHFKKQFRSSKVQDFQKNFHADLFQQNNPKQELFKLCLQNINSIFLQDKLTIYFKPIFIQLIIDNPYKIYDNLLQMLFGNEIQDNKIQEIIQTIKLNSNQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WAC1</accession>
    <name>A0A8S1WAC1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0860040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000086">
        <property type="protein sequence ID" value="CAD8185877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WAC1"/>
    <dbReference type="OrthoDB" id="283661at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8185877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="132" mass="15593" checksum="3C32066AD4F35462" modified="2022-10-12" version="1">MDTSFAGFTFGADELGINGSFSAQKHPRISKCSPFYRMNKPQTEQKPRLRLHSPNKENELMQIRKPVRVTTSPHSQHYQMSYKEICAHLIQLFKSQTKLYEQLMIADNLNIRNQLQKVQLKHDYYCKLYNQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WAQ7</accession>
    <name>A0A8S1WAQ7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0840102</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8184979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WAQ7"/>
    <dbReference type="OrthoDB" id="364224at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090114">
        <property type="term" value="P:COPII-coated vesicle budding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037363">
        <property type="entry name" value="Sec13/Seh1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11024">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN SEC13 / SEH1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11024:SF2">
        <property type="entry name" value="PROTEIN SEC13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="3 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="57"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8184979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="290" mass="34097" checksum="EAC5B3AD29505015" modified="2022-10-12" version="1">MNQQITQVSDFSFDPEFEIYTDYYQNKIAQVTNQGTVRIFELINKKDKQMKRLASEFQAHKGQINSMSWLSPKYENLIATCGQDNQIKIWKEQANQWKVEKQISLNNTQANIIQWAENKYILAVGYNNGYLEVFEGSEYQVTNTNAYNQTLLCLDWLKSDQEQIIATGGISDNQSIVKLWNVFSDQQTIIDQVYEYNCGDKIRHVKFAPQIFNQELQLAISYGNYVEILTLQYNIELIKPIELQKKQQIQFNNVCQKISWSLLGNELIVYVDNQMHQFYQSEENQYIKQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WC45</accession>
    <name>A0A8S1WC45_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0850161</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WC45"/>
    <dbReference type="OrthoDB" id="10251809at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14733">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23686">
        <property type="entry name" value="mS82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23244:SF501">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23244">
        <property type="entry name" value="KELCH REPEAT DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13415">
        <property type="entry name" value="Beta-prop_FBX42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="2">
        <location>
            <begin position="622"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="44"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8185569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="785" mass="91235" checksum="0DBDADD9F9C17C91" modified="2022-10-12" version="1">MSAATKGLIEFVNPYKLPKFVKQVHLQMREIEGRQPFGQGLYHCNFYENLIKRLTETRQQYRQSKEIETRKQLAQQEYQAWTNYIKERCLELPQQHQVTGKQLNELRRSYEVFIAKGENGLRPSELLNVFNDYTRVNQFTIPLDNWCVLQMVHYSMGYPMNMNRLLTFEEIATLVQTKVLATYERSLGQDLLFREICSYGYWNLFDQSKGSMNIKDFSNFIKIFKYNVEPTLGGILKEFGFAANLFQGEFVKEIDPKEDIVRFDFFRYLFLERNLMIWRKIDPQGKQPGDRAAHSCDLIMGKLYIFGGWNGMNALADIHIYDLNSNQWSELQTNGELPSYRNNHTTAVCQTKLYVHGGHNGNTWLDDLYYLETNGQHGQASWYKVHPQGQIPTARACHSLNIVSKKLYLFGGYDGQECFNEIEIYDIQENRWIQPTVSGTIPTARNAHTMTRYKENLYLFGGHSGAQHLQDLHVFNTYKLEWTQVITKGTLPKGLRGHTANLIQNNIYVFGGYDGSGRSNDLFIYNFLTQQWVIPNHHGTGTYLQMEEVALSQIPQPRQRHSATATENDLIYIFGGFDGNKWLNDLYVLDVGLLENRTIQEENYQRVISNIHKSLFNNKELSDIVFEIGNQKIYAHKIYLAAQSLQFKALFFSDTKESDQQTFIIENYSYKSFYIFLLFVYTGFINVAELDIESMGEILSLADQYLIDGLKNLMQKSIKKYISNETVCDLLIFAQKCSAHSLKNACMNHLLKNINIISESPKYEKLELQPSLLTEITRALLQHKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WD98</accession>
    <name>A0A8S1WD98_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0860153</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000086">
        <property type="protein sequence ID" value="CAD8186089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WD98"/>
    <dbReference type="OrthoDB" id="313524at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00821">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010926">
        <property type="entry name" value="Myosin_TH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34969">
        <property type="entry name" value="OS01G0621700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34969:SF1">
        <property type="entry name" value="TH1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51757">
        <property type="entry name" value="TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TH1" evidence="3">
        <location>
            <begin position="650"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="829"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="255"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8186089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="952" mass="113430" checksum="D94289E224864409" modified="2022-10-12" version="1">MFQDVISEFRNFYSNKNLTFEDFINIIDEYGKLDIRCQLEEQIKKQLYDFISEKQEGIVTYSTVETVIFEFLLQQEVPPYKQYILPKTLGRQAQRLPKDMLEVLTNDFIIQFKNLYKLIENRYGIDIEYEELENLGKELQRTIKINAIEMLRYIQPLVDEHKKVNIKELLELCNDLERIIINQHNNQQTIEVQESQNNEQIINKYQYISSDEEQQSNNHRQSEVIDKKDYANIIYQQTDILIQKKYVNQQGKQHLNDINQAVKKMEVQILNLQEETKQKDRKMETFLIENTKKIEEALIEKELAKTQLRQFELDIINKDQQIEILLQDIDMFQEQRNRYQEQCTDLQQQINDYHDQLKSITKIETELKQTQIEVKKLNENLIKSKTEINRLTQNNKSLMQIIEEYENKQNLEQEQVQVNVQDQNKDIIRDYEEKLIDLQQQYNLLEQTNTELEKQQNTYESQIIELKRELNHYKKLYENLNCENQQLRSSIMPRSSNIGKQFNLGGLGRMSKLSIVEGGFPSNKMGMIGRISSIRMSSIRGNPQIRYSNFNPLDDLQSGGLKINEQDECDQYEPDQQQQQPDQQKIVLRINPKYEQSVFESIHEGEEENDQTINKIEPNNQDQQNLLLQNFDKFWVGLKNKNQQESIFDPQSIQIDENLLFERDFLGIRDDQKVKEYLRIENNVHKQIIQRCFSDSIYRIDSKGKRARRIIYLSEYTFFIFFGEKKPRKLSRNFAIKDIKSIIFSETSPVVCCIKVAGCDDLLMETFKRTELNIYLTEIFNQQRLLLYNLEFQSEFKIKFIGLNNEIPFSQVSKRQGYLDQGKVSAFKVSSKQGWLYLLKMNFMNSERWKEVFVILTNVGLILFKKPGDFEPILFVSLVDAIVIKDPIIQTHQQNLLKVRYENSEYEYLFSTTSAILKDEWHDIIQQTIMEQVRNQKKYSFDQIERSKTLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WE08</accession>
    <name>A0A8S1WE08_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0880072</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8186950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WE08"/>
    <dbReference type="OrthoDB" id="310138at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8186950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="115" mass="13341" checksum="A63CAD251744B064" modified="2022-10-12" version="1">MQITSPTSKAIQTNLEPKNSENQNIEFICQELKNICTVSYQLISPKISQKPIKKKKIIKKVNKNDISKQEIGEEQNNQSITNYLLDTQEELKCKIVLIDDLQQKLLFLKMMISKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WGU4</accession>
    <name>A0A8S1WGU4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0930117</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000093">
        <property type="protein sequence ID" value="CAD8189268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WGU4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="184"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="370"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="411"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="433"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="462"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="502"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8189268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="556" mass="65736" checksum="18461AC223AFBE62" modified="2022-10-12" version="1">MISLDEEECDDNNNEDGDGCSRFCKIETDYQCKNYEFSFSQCTYSKQPRFYLQFKEERDQKQYVSLLFTQQVKNKIQDQYSKYIQMNIIDMDINIYKLNLIIIQEPVDYPTIVEYLIEIQINSTLNDRPYLEVTLDEQLYNSEDATLFNLYDKIQLKQPKYIDDQKQEAAATLNQANKYLMNSIGGIGIFAFFLGNSFIFSSILEVLQQQSYLRFINVQFPFNLFIYFESSNIITIQPILKFFKVDSIATLTFDSTYIESYEKLKYYEINADLLTNIQTQVFLITALIIIYYSCTLIIFLIDSIIIQFLFLIGKLGYKLLFKIRKLCSNYQKEFRKEGVKAFLIANCWDLLFTCFLQLKSSQYNTTIRSIICLILAYLILYGCILILRQFIYKNQLIKYSLFNYWISKMDLFLTLKKLIFIFILVCFQKNEQIQTILLTFVCNLYLSYAIKFKPVKNQQDFCNLIMMEGSTFIFTLTSALYWTQISKIFVYENQVLLGWVHITLLLSVLIVNLLLQVFALFLQLKMQNQNKQKKSNQQNGVLPHPYPLANLMEFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WHR8</accession>
    <name>A0A8S1WHR8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0900003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8187709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WHR8"/>
    <dbReference type="OrthoDB" id="4869960at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8187709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="65" mass="7836" checksum="76E849A73ACC8792" modified="2022-10-12" version="1">MHVYEMNSTNKQHSKTKEIAVRCDYWGCHNYFFQQYIKSKCLLVNKNGYNVNQIRKNQNSDFITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-02-22" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WJ01</accession>
    <name>A0A8S1WJ01_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0940001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8189393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="613" mass="73147" checksum="C136974A6A0266E4" modified="2022-10-12" version="1">MMIEIMKQAMKKALFQILNKIVRKNSLQKLQFKLQKQKQLIKYKNKSLSQEQRNKINYKQVNMNQQSISNKIIITISSQSIYRNLFNYQKFQIESKFFFYXIKQIGITEFLKQIPTVTVDGLKKAGFWLSDLDKVNIQNQYQTLQMIQQTALQTQNNNQILNLATEVLGDQVRNGAISESTASNLYDLVNQCFQQSKGTVNGFRNSFCELAKKYLRLQIAKSKSSKSEKNLKKEVEEICQTQGNKKFDGIQSKIHQYQQNEKYLNNEIQKFSEIFKIIYLKDNELLKKNMEKEINNTNIQKYLNSTFDINDKDIQDECNNVKISDQQSMNKCYQKVIRPKIMEICMDVYNKLGEQCNIFIDCEFMEPIEIIFENQYNDHLNSHIKKFKSQTSEYQMQNASQYQIQQIQQQCEELQQITNFEPKLLVKNAIDYMKNQQMINNDGITVSNQFMNKFNNYLNQQNYFSMMIEIMKQAMKKALFQILNKIVRKNSLQKLQFKLQKQKQLIKYKNKSLSQEQRNKINYKQVNMNQQSISNKIIITISSQSIYRNLFNYQKFQIESKLFFLNHLILIYQNYQTYIYFFNINNYRNYKSSKKSILETNSQKQYKIDQFIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WJL3</accession>
    <name>A0A8S1WJL3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0940144</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WJL3"/>
    <dbReference type="OrthoDB" id="5337378at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="15"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8189673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="458" mass="54935" checksum="FE11963C3F391156" modified="2022-10-12" version="1">MSGQQEIREQYRIDTSTKNLIGQGGFGSVYKCQRKDGSDNDSLCIKIIKKDRNQMKQLKQEIEIQKLIMNLKTDKLLRTENIINTPHYFEVIMERCDMNLEQEFGNLKNKQQWYDFNDCLNIINQISVGCRILYEHNIVHRDIKPSNILVKLENKGQINERKLYKISDFDFSRILVDFLDPDEITTVGTPCYESPQIFYNQKYSGKCDIFSYGILFYQIFFNGQLPFNSIQNGKKEIYEFMKQLYNKQFKCELPNYPRAKEIADLLQKMIVYEEEERISFDQFFLHPIINLEEYEIYKIQQVQFRKYLLYNSICEKYENQDQILILSAVFCLQLLGDQELLYCMGFIHIIISDIHISYKQNKDITTIMRILQQCAQNINIYPQYQLLTKRIKQAYFQLKKKIFNLREQFRQKLNRSYNKNPFITEITQILEQSQYLRIQPQSIFDQLRSLLDQNLQEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WLC7</accession>
    <name>A0A8S1WLC7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0940017</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WLC7"/>
    <dbReference type="OrthoDB" id="323570at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8189421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="116" mass="13686" checksum="4116D82617514A7D" modified="2022-10-12" version="1">MIKCQSIKLKDCHYSCGTCGGMEELDCTDYRYQVGNTFFCKEGYYDAGLPVCQRLQSNLKVVIFIRYNFNTVRQFVSGLNKFKCLQKQQDDRQNQVCLDYYIINHQRMPLLRSLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WM53</accession>
    <name>A0A8S1WM53_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UBA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0940153</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WM53"/>
    <dbReference type="OrthoDB" id="298527at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBA" evidence="1">
        <location>
            <begin position="26"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8189691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="254" mass="30318" checksum="007C07D981E754EA" modified="2022-10-12" version="1">MINQHPLYLEQQQWLAQFQKGKQEPEVQQDLLHELLSMGFDDQKRILKQIKKHKSDKAKIVETLLQKQIKKKKSDEIDIVQIVKDTQILYVDCNNVKYANRNWNEFHGKQKEQIVKMLEIIVRWLQNDNSQLYEVHLIWDVYKIKNIQKLQNEINQLDQFKNLINLSQEGKKIQFQIEFNNKKILFTIQSSHPIIADDLIVNLCSQNVQKSKETTVVTSDRGLRDRLKEYGIQTFIGCGKWWKLQFNDNQQQIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WUV3</accession>
    <name>A0A8S1WUV3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1050091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000105">
        <property type="protein sequence ID" value="CAD8193884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="299205at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="254"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="285"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1089"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1141"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1279"/>
            <end position="1304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1418"/>
            <end position="1439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1668"/>
            <end position="1690"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1702"/>
            <end position="1723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8193884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1724" mass="202355" checksum="C5CCCA71779AA8A0" modified="2022-10-12" version="1">MRITLIDNLTIIAHSQHQNQAGNTMVSVILIIPYIQYLSLLLPSHGWSYWNYSDGYLSFIQKACSYFTITPFFEFQNNFIYYPFTIFSLIIMVGLILYLSIFTLEMQSENKKVKYQEGRTSTLCTVILFTIQTLQIPCYKFYIQQIIEAQQKNLQNQLIINIISIILYCMYIFMSEYFLRQYSFAPYHPMQQKFTRLRVSQILLNLIAIVLTLEQESRLYNLIGLLMLHSIFVLKMLNHLYYKPEAPNKNMIHFEASFTLESIAILITVNVFSENQIFPEQQIGIYIMFIFSLGMILGNLLFTFLLQLNLDPKTRQLYQIYKLYSYIGNITSNSITTYNLIIYQLINDKDFTQFQQKVILNQKSTKMQDYYKLGLYIITEIFIHLKEQQKSELEELQLLFVSYLALIRNKPLIGYVEFKKFESNINIKKSYYFQLMKSRIDQHLQKKITAIQQIYEKEQSYLKNVNLQKSVSPAELYEYSQIEEKFQAKVSNLIQLKISIWENQISGCKSIYEFEDNAVNLSKQIIECIFFLRSQTINVIQEEYIKNCDNILKLKICSMFHSFVLNDYYSAYLCEQKIQDIMKIETTQELNVITRTNILKDETILMIISIVKQLGQILNSNKSQIANYFGYQNSEFQQIANIKELMPSYFGNQHDQFLLNYIKEARTDLAFKETLTFAQGKNGFLIPQYLNVYNNFDINDDFTLIGSLTKEKESHNYLIFDEYGKCIGITQQLSKLLVPQDNIEYFLANIDSFYVYMFLPHIHIYMNDILNNSTNNDGAIQQKSVLMYVFQDFVDLQKMHQTIFNSYQQPSQKYVEQTRAFSQFEQMNSLITSPRNGNLKRLISDTKPSHQMEDVKNLEQSSLDFFKIGQQQKQNTLQNANANSIKENEIIDFLNAIEVLQKTIYFCTAKIQLTSLGQKENKQHYFILELSEFVDKSNQQDEEKKQIRRQNLPIRRTVQRHSSHKQSFVQQFQTIPKSPDYHMDSSLMSPIGSVIQKKSFLDKSPVNNQILHYVSERRLNLMGSLGQADSQSFSEELFSNEIQQVLQHQNEYKSNHGVGILKSQSTTKSGNSGLTAIEIVNKFKTKTSLISSLAIISLIKFVILILFIIFMTINLSQINIFNQSVTKFITDINLPINLNKYFLNLFTYSWITSLVINNILNTSQFINNQMYIQSQNMETTFINLTKMYSNFISLENNGYLDDLNITHLDNTIYVEKVDFTDYIYYLQNIGYRLLHFESNSIYLSNLLKYRLNFANIIQNNFQIIQSLGSYYTDQQDGKILLLFNQILVEVIIIGLLILSQLYFWRRIEQYCQKILLLSNRLTENGAENQIVKFRLVGQIIKQTYGELGFKQQNCYKLCYTDMLQSKKGFKQHRSKIQSILIKSKKSKQQEILTKKPGSTTIPLNSRISNVQINIMLKLVYVIINISFVCFFFLGDYLLYKDQSLKLNPAYELAMDYISFQIRFENAISVALMIKSQQQIYSVMDEVIRNEKLILDSLNEEYNVTAIISDAYSFNHSDLSSIYQKIIDYNIINDEDETYILKLYNNDFCLIFDGNQIPFCNKELSSQQFRLEYGTYESKDNNSQYLKSGIVGIVSKMDVFLMQYYEYEYQTGKMDNDFVKLNYQLNSQDFNNIIVQHYLDTYLGFEEFFNKIQESIINLITDQQNIQDLYQYLIGIFIIVFLIISGGIMIIKVNLRLIHLRLLITLLPVEIMLDIYTISLLKILQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WX79</accession>
    <name>A0A8S1WX79_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">STOP protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1070090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000107">
        <property type="protein sequence ID" value="CAD8194644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WX79"/>
    <dbReference type="OrthoDB" id="285423at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8194644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="262" mass="30712" checksum="FB9C6179DAC261A4" modified="2022-10-12" version="1">MNIKQQLLNQQIQSYQHSDGTCFCSVCLCSKCYCKQKPQQTHYINEPKSIYNIDYDSRRTPNKHSRLPNQNYQGRQKALSLHSVYNDDFDKKQLNKSFLDNTQYPQNNSFSIPFCGCNTYALQFPGKTSMPPQLLKPLNQIQLLPGKLDIKSSYQREHKYHKLDQDKIINFSKITNQRLHTNSKEKNIYLNSSYQEQYKGQIAKKVQQLPIQQSYLPFGLSNQGINNHYVSTNKKDYEPQSFYQCEADQKLQKLSDQILSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X139</accession>
    <name>A0A8S1X139_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1070078</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000107">
        <property type="protein sequence ID" value="CAD8194621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X139"/>
    <dbReference type="OrthoDB" id="291503at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035865847" evidence="2">
        <location>
            <begin position="17"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="55"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="286"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8194621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="368" mass="41959" checksum="5CF4FD2706F092B0" modified="2022-10-12" version="1">MQKLLALILSLSIVYSIDPSMVQLSTEKMDELSQSNLGKFILEMAQTHAEMRGPLDDLVTAIGDLENELTAELQQLEDDFTRSTNQHQVTQENLDINIGQTEINIFNQKDFIDAILLPSIDQTNSKIERLNGFMNDNRDSLSRETLARQNQHQAYLDRVSEHQGAISAVDEAIQLINSLINGNISFSEKATIHQAVKRVNNKIQKTSTIHPLVEALLQLTQNFSDQGQAKKIKDLLQDTRNQLVSSLNQENTDEQQIQETWEARQKTLNTEYQEFKRSVLEATYVLAAYQNKLKSTKEALEQNEQDLQNYQESLQQDKDAQAQEIQIYNELKAQYQIQLQTTRNAKEFVNSAEFSTVIRNKLNSGGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X3C5</accession>
    <name>A0A8S1X3C5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1020142</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000102">
        <property type="protein sequence ID" value="CAD8192736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X3C5"/>
    <dbReference type="OrthoDB" id="310229at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044548">
        <property type="term" value="F:S100 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06468">
        <property type="entry name" value="p23_CacyBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052289">
        <property type="entry name" value="Calcyclin-binding_UBL-bridge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037893">
        <property type="entry name" value="CS_CacyBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13164:SF3">
        <property type="entry name" value="CALCYCLIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13164">
        <property type="entry name" value="CALICYLIN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8192736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="188" mass="21778" checksum="39806BC9CC4D8154" modified="2022-10-12" version="1">MKLYIHSGNAVEIYESKQGETIDQLQKALGSEIIFNNRTILNTSMLVNSFFNDNDDVIVQKVKPIPVQQNVQVAAQKPIHYNNITKFAFWDADDMNVRVVVELKDIAKHPLEKFQARFFERSFEIKIHDYQNKNWTFGVARTQCKLDAANSKFTLKGDKILITLRKIKKEDNWFSIHKQKTIGGDDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X6E7</accession>
    <name>A0A8S1X6E7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1060078</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X6E7"/>
    <dbReference type="OrthoDB" id="296255at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="285"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="513"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="647"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="689"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="762"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="11"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8194206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="807" mass="95104" checksum="5D172D840B7D93F8" modified="2022-10-12" version="1">MSNNQYNNKKLAPFKKQRDTLKEELKRLQQEQTEEEKQNDYYFDEIGRVEKEYESLIKSIEEYSKHNQTLKTIRQTDQNQGLDETIEKNVIQTLNLFLLSTSKKEYLESIQEQEISKKQLQVGNLIMTQNQLNEDNQKEQEIEKVILDNGYYITLKQGSSSLIVKVPGTVRTFRELKQIVKSCCMAEEQEIFYTDLMGNILQPEMNVLDQLYPPIYELLKNYQPVICIQIVKQKKVKEDTKQTEADFGFDGGIQELMTKQLRSIRRIEKSINWSKYMDYLTSFKYVLESILFLSLLILYAIVEINENDFFTNSQLLLNLNMNMAIQKSYLSPINNISQIISLTIPNGSSHGLSPTYPLQSGLLVQTLVGIDDIKNCDILNENQKQIYVDKNQSCLMFDITQTTDLQKNYSRLNFDPKVYNEQFGGYVWEFNMSTRQSFQESYDLMISEDWFQYNVKQTQFILNYYNSPTQRVVSVTITTLYLFNDRLLNYNSFQAEGFNLSELPEQVLVSKNIIFYCSIVLLISSFFDFFGLYFEGTKNNLILIYLQYLELLNRKKKMEAKKKQINENDQREIQQKELIVGEYVIINLKVIYITIRIPLVFDYIYILCQFGMVMKNTVDSYYEEALKEIDLNSDQYQDTNALIEPLFVGRLFSAILVIFLMISIVRFMGNWSPYLKCYGLVMIRFNKESWFLLLVLMYIISVCAMSWIVTIQGKLVNHDNFFYTFLGLLRCTLRFGLDNDLEQQGFYNTYAKDIIYSFETKYLQYIIIITITMIMIPIFISLMTDLVQNTKVEAKQKIMEMRKQNQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XFX2</accession>
    <name>A0A8S1XFX2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1220135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000122">
        <property type="protein sequence ID" value="CAD8199761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XFX2"/>
    <dbReference type="OrthoDB" id="10398345at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8199761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="196" mass="22723" checksum="531C52FCB9759D3B" modified="2022-10-12" version="1">MIIEKSMQLYTAFLTIIITILFVNIILLLLLILLSWKISCNIKIKQQIKTPQDEFKIIQYQEEKCLEIINKKEEQLKNPTPDQQEIEDKGSLNVSINEDISPAMLKLKSSLSPSPGRYRSGKILDFLEKKLSHAKTSQKELHNVYSKEFSKKYNTNQALQISVDFGEQEDVSSSIKNKIEIEINNHNFFKQTKFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XIZ9</accession>
    <name>A0A8S1XIZ9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1240111</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000124">
        <property type="protein sequence ID" value="CAD8200412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XIZ9"/>
    <dbReference type="OrthoDB" id="304129at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="342"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="616"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8200412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="743" mass="85835" checksum="DF2A370FBC57B4D8" modified="2022-10-12" version="1">MDNSFLISIALLISGILILIELIVFNSDINRLQLTITIGQIIASCVFLIKPYQRSKIVFLISFINVQQILQQTNFAFLFIVLTQILSCELEIEIELNTAFRVLLIISQILRVIFFWDNDIFEGAQVIISILFSCILWMKSYLLSKIKKNGNIQTTRDHNNNLLFNSQTNLIVSLKQLDSELGILDNLPIGIMILDLQQNIKYLNSFAKQYFKNFDCEKNDEISLQLKHQLIKMLLDHIGGPEDLNNLHDNQKSNRPFLSSIKIDDPIQTILNQKEEQFNLVYKYKELSKNGISKLKVVKISLLQQFLKQEEITILLIQNVSSKERKKELTQFIKFQNSILNSFSHELRTPLNSSLQLLEALSTRLNDQMNQEYVQPALNSNRLLLFQINDILDYAAMQSNHFIHHYSQFNIKEIADYIKQLYTQACQNKGIILTCKINLTNYIVTNDKQRIIQVLINLLNNSIKFSPSNTHIRVQFKNKFKYSQKYIKIKVKDQGFGISEQKLSFIFRELHSVSSEESGYWMTQNQLSSGIGLKMSNRLIQGLAFTEPRKNAFSIKSALQQYTYISFYISDMIIIKEENQSFSNALSDDAHCIQMHQSQDFKLIENINIKSCNCSSILIVDDVPFNVQALKTILYQHKLSIDSAYNGIEAIDLVIKKYQQNKCHPSYQLIVMDIEMPLLNGLQATQKIISFFNSINVQPPAIVACSAYDSNKEEFSIFSDTLPKPIDQQKLKTILKKYLSNFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XN88</accession>
    <name>A0A8S1XN88_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1320041</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000132">
        <property type="protein sequence ID" value="CAD8202780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XN88"/>
    <dbReference type="OrthoDB" id="2344588at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00821">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051707">
        <property type="entry name" value="PI-Interact_SigTrans_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14336:SF8">
        <property type="entry name" value="PROTEIN OPY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14336">
        <property type="entry name" value="TANDEM PH DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="23"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="208"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="873"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="593"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8202780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1063" mass="126028" checksum="8A80D469E53936E1" modified="2022-10-12" version="1">MSESQFDGTKFRLSQPESFNNKNLKLQGWLKKKSPAILKGWQKRFFMLIENEGYKLVYFDDDKTNSKPKGAFELSNNIFIEQAGEEEFTIRFPGRNLELKAKSGDERQKWVETIEFCKSIGKNSQRKYLSVDLNDNDLSKSKGSTHKKVDLSTTQIVQIEKEKIENQRFQSHKEIQKKSIILSSQLEDSYILEFLGLQQLNQNSKLKSRTIFGSLSKRSKTKMKIYKQKWFILISSKPLFPEIQEDEEILQQQDINLELDTLIYINQKVNEKLIKRKAYIKLQNCKEIIIKNMQKSSDHGFIFKLNMGDQIYSLMANSENDRMRWQHALNESMNTCKEINNKLNLNILKNINPIIRIYDMESSLPIRRQKIKEKLQSDIERIELEQSINNQNRYLNLQDLLCYLKLISQDMIKSIEGCLVKDPQRQDIIQEYIEIHHEKVCQNISEFWNINNKYLSLNEIKELVTWLFDYQQLIINYIKDDRIKQGINTLLQICISRWKTQSEKKIVAQFEQCQTIKAKNTIKTTCVFELFDTVQEIERLINLKLKSYLKINELFKIIKSVLWYYLEQLYDCVEEQTENQEFLIGVCNDVEIVKEIANKIESIAEMLQENKKQEKIKQKILQKYNEVLEFVEQQIYNLTLNELTQQFQVSLLEIEIRSRIFELFEGNVQLIKLTEQTQQTLVEMLIQTLNYHYFNIFLNELDKVNSYKRKQIESKIKQDKQVVSEFLKKYHVSNAKLETFDLMIQLLNCEKDSEYVQTFRGLKYQFGRCFTVNTVKVIIGHLKTRKSEINQNSLDQFDQLIKNIDDEQENGESTQDLKSQLMEFGQNQFEPLVLQKQDSNSSRTSRTSMSKSKVNIEELISKSQAQSVQLDNMSISEGYVYKKKAKQQKLVKKWDHRYVRIRKGYIYWYVDEKSWKCQNKLNVQDILEVEGKQNKQNKFILKMKGGKIYEFKVDTQEERNMWMNGIMNEKRQQEDVFNSKIEAQQVQTSLLNVLIKNYDEEIITNKNNNNNNNNNKFNNQQRKTTLIIQQKEYPLELGQTNLKIKKTSNCWTSFMVCLGLSKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XN91</accession>
    <name>A0A8S1XN91_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1310027</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000131">
        <property type="protein sequence ID" value="CAD8202417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="292058at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332:SF175">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035945122" description="EGF-like domain-containing protein" evidence="5">
        <location>
            <begin position="22"/>
            <end position="1823"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1414"/>
            <end position="1439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1508"/>
            <end position="1529"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1560"/>
            <end position="1577"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1618"/>
            <end position="1638"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1672"/>
            <end position="1690"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1696"/>
            <end position="1716"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1761"/>
            <end position="1789"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="56"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="98"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="135"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="174"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="227"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="312"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="391"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="446"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="489"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="535"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="586"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="630"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="675"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="726"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="765"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="811"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="887"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="918"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="948"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="993"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="1147"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="1189"/>
            <end position="1227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8202417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1823" mass="205212" checksum="2AAA689A1D5E71B8" modified="2022-10-12" version="1">MRQNNSYFLLILSGILYQTICCPSSCQQCNGSSSNCNICNPGFFKIGNDCYACTKPCATCSTSVTTCSSCVDSANQVTPSCACNSGLIMNASTFFCEGCPSNCSVCSTTTICTQCNDGYWLNVNSCSPCIKPCVNCSSNGNNCSSCVDTAKQTLSLCNCQSGYIMDTTSYLCIQCVFPCATCQTTVNYCLTCAATYTIDSSTHTCSCLTNQYEVNATTKSCQNCTSPCATCTLSATNCGSCVTGLNRNLQTNQCVCDDGYYENTTCQPCQEPCIYCTSLSVCTSCKDPTYQSGSSCVCQPTYFMNASFMCQSCVAPCQNCTSETLCTSCVQNYYISGNTCLQCTAPCYNCVDLPTKCTSCVDSNITPINNVCSSGGCSDGQFMDASLNCQPCIHPCLKCQTNGNHCLECATTFEMSTSTPNTCVCPAHKYLVVTNNPTDCQSCTQPCDTCSGTADHCLTCIDNINQTVDASNECKCNPGFIMNGINCDACLNPCLQCQGTTNYCTECKDSEHYISNGQCICNPGFINDNNYDCIPCQTPCSTCSVNDTHCDSCKDPNHQINASFQCICKDTYYSNTIDTCASCVQPCALCDINGCLSCIDTNQIINSSKNCVCKPGYYQVGLNCSQCVTPCQTCEINQDHCLTCTDIHQTQINNQCICNDGYFDVNHICQQCQSPCTKCQFSNDHCLQCLDPNHDLINNKCVCKFGFGQSGTDGTTCSLCQYPCLDCSTSVNTCVSCIDTIIFHLDNEKCLCQQGYFLTGSNCIQCSPQCSSCIDQSNKCLGCSDPNQVLNQNDCLCQPGYFKNNSMNCELCQLPCLTCVSNEDYCTSCYDQEKQQVIGGQCVCIDGYYILDNKCEKCNQICNKCSSYNDCSECIDGYYLQNQQCLKCQTPCLSCFDAYNCQTCIDNYTMNNLGICIQCIQNCKNCIDSISCISCFDKFYYENQSCIPCSNKCQTCENDSNFCTSCNNTIQILIDNQCYCQDGYFEDGQDCKPCDQMCKRCQNLQHCTECIQSDHVILQMNVCVCQDSYYLDNKSCQLCDKKCKTCTQKSDNCLSCNSQMNRLKQQNTCICQDGYFENDNYDCWPCDSDEGKINKNCRYKNCNDKVWTYEEECDDGNDDSRDGCTNCKIDEQYSCINILLQPSFCFKCSENCQKCQMIESKNLSACIKCHQGYFLLQDKCIQCSEKCLDCKISASNCISCKYQQNQQGECQLCQSGYYFDQINNKCNSKCGDSFKTIEEECDDGNLLPGDGCNEKCKKEKKFICKDGICIIPEYPVPILLSYGDTQLYNKVRRFKLDYNLFLNISQESQVEKMIKLFIKNQMNLYPIDCPYEMQANYTLNNESKMSLSIDFQIFFNRSTTKEELLIQYLNPSDFISYQGYSQVLSEVSTTIPEYIFIDQTQATQVEMAASSNQILLYIMAVMAGGAILFGGLDIFYNLLDTIQMLSYLKYINTQFPFNLQSFFEFFSFAQLSFFSKYLNLQELIDPYINYDDLYQIPEKIQIDGLNSLFIINGTSIIFVWITLLAVYTFSKYLPHLLHQIKLKYYNEIPGDFDFALKCKFWILALKIFIVQLCLTVVQEFFYSGILRTFFATAYDYSFSMTLQLYALELNSKNLLVSFSSYLALLALGIYLFTIFIIIRKCGSQKFSLEEYQNKLKFISLFEGIKNDYYSKYFYAISLAKKLIFMLILIFCYKQPYFQVINLILLSILEIIWLLLFKPLEDINEFLKQISCEINKSIALFFISGLVLDQEVKLFNENIRSTIGWICVGNISLILIIQLIIDAIQQWIFLIKKYKQFRRLVEKFYRFLRPQQQRAPHSVFIFAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XND1</accession>
    <name>A0A8S1XND1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1310057</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000131">
        <property type="protein sequence ID" value="CAD8202477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XND1"/>
    <dbReference type="OrthoDB" id="302536at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035851100" evidence="2">
        <location>
            <begin position="18"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="855"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="886"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="915"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="954"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1006"/>
            <end position="1029"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8202477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1062" mass="121078" checksum="42BDE7975B8F5D23" modified="2022-10-12" version="1">MNPLLTILSTLLINSLASVIIVYDQKNDTIQQTANTITFNITAIFPTSSDIQVDVQLPKQIIGQENIVCGVTGLTNGACNLVYSKFIITFKSATPTFQIIFINVNNPITTFTDPVQLNASVISTKSVIFTEVVQLKFQLKNLPISINQPSTTYIQAPLIINIDGTSITYPTQSQLIITTNGIMDNQAKLSGIIFDSIILQDNKIIITNAQLLSNAKSSITISKFMIKSNEPINYKIEILDSNNGYITMFSTSQVVQAINSNYDYTLSTENQVVGMPTAIKVIICNLDEQSKFIFTTSLVKLNYVTSYESNCHNTLFQQFINPLSLNTISFDVQVTLNNYDSIKRSISYTPVKDNIQMTMSQNTTIMLNYALVILSLQLNQQFSNFYIELINSYQTNSCKIQDVDCSQINNIYKSNLLEQKLNKVIIKLEILLPGCDSYQFQTKLIYQNQELAKSNQVIPQLVKYKLASTLSTSSFNIGEESVFTINGLDSTQITKYYISTTLTDLKCQDCISVENQLQYGQVSNIQFSANNFQNVKPYTITIIAYKDQCLIAEQIFRQSAIPQLLSLSVITDNNYQQALGNWQVTIAPDLQDKGILRTPFILPNNQENKINASSMYINQTQYSLNMTNQYFDKEQNYWEVGYYRDGYLYAYGNLTLPQLDIMEFKNLKITKQNTQINETDNITITFDTYFDIPVNGKLQLDVDNLLLSNFTLNGEDINNQVIIAKGNQVLNFKVSFMNPISNYSEFFNLTAFNELGRILQKSNIKYTFNYGCSIEQCGVCNLTDCIACKSTFTLMNNTCQLICKENQIIVNNTCVNNQSNNNNSNNVNDTNHSNNDTINTNKTVDIAIQNGEDSFVFTPPIISPIVILIFLFALSRKFWSSESETFIIFYSLASIMEHIIHIFLFIYFFVTLDLIYGFCMISVIVLHFSSQIYIVFMNLFKIDPEYIGIRQQSIWFKMLHIISLTFCGRLLLLLISKFDQSYVWSTPWTFKISVLQRLSNYLKFQIIAQIGVLTLVILVILQKLFLLYIEILSFQIIQTVVTLYTFNHLRNQKNQEIRYTYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XNF1</accession>
    <name>A0A8S1XNF1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1310081</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000131">
        <property type="protein sequence ID" value="CAD8202523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XNF1"/>
    <dbReference type="OrthoDB" id="9547406at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141052">
        <property type="term" value="F:histone H3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003349">
        <property type="entry name" value="JmjN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004198">
        <property type="entry name" value="Znf_C5HC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF33">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02928">
        <property type="entry name" value="zf-C5HC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51183">
        <property type="entry name" value="JMJN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjN" evidence="4">
        <location>
            <begin position="44"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="5">
        <location>
            <begin position="191"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8202523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="500" mass="58042" checksum="C0ED68FAEC858FB4" modified="2022-10-12" version="1">MIHTKKHSKQTKKSKTSDDFTEPHPKLLLERFPYTSKDFQLKDVPVLAIDQSFFNDPFKMIDELYSKGYEQFGIVKLILPSDLVIPNKKFFSQLESKLKGKRIQTRIQTLNTQRAGEIFGSNTLGFTIQEYMNLANKFECSHRLQGVREVSNQIRQNEIEFWSIIDYPTKYEDIEVEYAADLLASKYATGFQEGQLGNLNGINKNRNSIFQVLQEKNEINGISVPWLYLGMKYANFCWHKEDLNLNSMNYMHAGAAKTWYAVPPSYSDKFLQFFNKAYENERKSNPHLLYDITCQISPIELIENGIPILRTDQQPGELILTLGATYHAGFSHGLNCSEAVNVAPIQWLKEFDKATQEYRMDGNLKKVSFPLEWLLCKVALMAEEIKFTKQAWQKLFEKFKQMMDQEINHRNCIISLYDHVKIIEFANKQEKYDRSVCKICSNYMYLSYIFCGKCLKKGCICHQSICACSSPQISLYNRYNNEELQVMIATLESKIRTTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XNI7</accession>
    <name>A0A8S1XNI7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1310106</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000131">
        <property type="protein sequence ID" value="CAD8202573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XNI7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8202573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="280" mass="32522" checksum="44F77C07EE372AA2" modified="2022-10-12" version="1">MIRASPSYSLDNSFQLNFLKKQICKPIIQIKSLVKNRKTRSKSQEATDSDQQNFSCIQGPSRRKSCYCNQCGCMGKKQLQFMDLKLSKQPKMINKRAQCNIDRRCTLNIQSINRSVFYRRNTQKNSEKVDNLKSPTYDLSDSKSPGWFIRQMVMRKSTINVQKLVEKSHSKYSNQQSNKKIMVDDLLSPKSQQTIHLPTSTRTVYQKCCNDNSCVRIRTVAGFDCENNLNSPNHREIPKLSPYLSFRQINFNLLVPLKLKSKSTSRKTNKKIFFHSKVQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XQ02</accession>
    <name>A0A8S1XQ02_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1330081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000133">
        <property type="protein sequence ID" value="CAD8203205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XQ02"/>
    <dbReference type="OrthoDB" id="285586at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8203205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="161" mass="18713" checksum="C0B6F17581C61B84" modified="2022-10-12" version="1">MQEIEAKKQLKASEGAHFFYTLIFLSASGIIETQFIEQKCNQNLQLFVHLVFYGLIIWGTYILITLIPRYKNAAINLFFNFLDICFGIYIGLLLFYGGRMYMASNDCQTEAPVLYFFLETFLLVNGIIFGILFLAFVSYVLKRFSKSQQVYDEGKDEFYEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XQ21</accession>
    <name>A0A8S1XQ21_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1340058</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000134">
        <property type="protein sequence ID" value="CAD8203556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XQ21"/>
    <dbReference type="OrthoDB" id="300132at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="259"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="97"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="324"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8203556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="561" mass="68695" checksum="2A0335489DFE1930" modified="2022-10-12" version="1">MSNEIILETVRELLPDIEKEIREQVSQELQIEYQIKFQQELDELTQFNEEQIRDELEKQIRIDYEQQKEQLAQDVIREFDDSLQRGQLEQQLRFKMQEELEEQINEEVQRKSKQIRLQYKKKLEQEKNQLQKDFELEWKQRVDAEKKRLEREKSEIARLKSVEYVKLKKLQDEQKKHSQLLKDQETKYQDIMKSLQKQIQELTQQLEIALQDQIIQEKLRSQQENFDSLDQDKQINIPLEKQDQIYNHKSNPIYQHKQITEDESQESSYTQVNLHKQKQKNNIQKSDQQEQHNQQYLKNQNKKQRTQYQNQLEKGVKQQKTHGETSQPQYLEQENNQKQMNRIDTIIKQYDEIPAQKEQFMSYNSLFKENDLDNSKNKQMKQRRQKFVENQDQDQRLQTKYLGIQINIMNDFIVDYVEFSEYHQVKMMLQEIIELFNQWMISYQERIEFFKRVKQIANSNFRDIIPFIQTQIHKFKLIQNYYPDFQKRFNLKNQIMANYSQFSKCKHLFDQLNQLNLKSINISVLGCDFQEMLQLDQWEKDFLEREELKSQILCKIQSKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XQ93</accession>
    <name>A0A8S1XQ93_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">IBB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1330062</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000133">
        <property type="protein sequence ID" value="CAD8203167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XQ93"/>
    <dbReference type="OrthoDB" id="295733at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002652">
        <property type="entry name" value="Importin-a_IBB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01749">
        <property type="entry name" value="IBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IBB" evidence="1">
        <location>
            <begin position="4"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8203167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="485" mass="56938" checksum="338F07290973BF8B" modified="2022-10-12" version="1">MIVKQKREVFHVQLRRNQREQIFSKKRILDQPQQQEAVTSEQAILFLKGGMSNQMNLSEMFQELHQLLPKYGDLHLKTLPYIIQGVKKYLQDADSSLLYMNDEDREISEYTVLEILQYLGSLTYHIKVEFDLDNLIEMLVAILNSTDEFKMIERLCQIFQNLLFDFEDASSYLLKCEIVTKLANKFLDCNVSYDLNNLAPMFRVLQQLDGVNYYDFNQIHPLLHRLQNDSKVDTQMYCEIIEGFFSFLVKCTNQLTIQDDSQKLLQFQLFNKLFEFQYVNWSVNLLYKYKNQKKLVPVIYSFLHNLSLGDCGKKLISQGVLTAIFEHLSDKIVSQNQLYSILTNLIVDDFDSVMKSGILKKVHSQFEQGFYSADYINELMYCITNAFYTASESQILELIKLRLGDCLILYISQQPMELLDLELCSAIFQAIHSVFVKQPQKSVYSLKKKIRNDSIFLARSSQIYDISHKDDKEYEDMLKYLNKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XST3</accession>
    <name>A0A8S1XST3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1370124</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lin-54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000137">
        <property type="protein sequence ID" value="CAD8204526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XST3"/>
    <dbReference type="OrthoDB" id="46687at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005172">
        <property type="entry name" value="CRC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028307">
        <property type="entry name" value="Lin-54_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446:SF34">
        <property type="entry name" value="PROTEIN LIN-54 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446">
        <property type="entry name" value="TESMIN/TSO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03638">
        <property type="entry name" value="TCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51634">
        <property type="entry name" value="CRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRC" evidence="4">
        <location>
            <begin position="154"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8204526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="278" mass="33105" checksum="E6149857432FD40F" modified="2022-10-12" version="1">MQYKSFNKKVMHQNNFWNQSEKRKESNTMTYYYQNTDKFDINLDDDVEQLPMSMPQLKNIMSILSDKFNEELESPIIMKVQTENPLEDVENDKYIQEPNFKIQVQPSKQPKELQTQTLEHRNRRIHKQKINTTLKRNLEFNQESDLNSLSNEHTTTPCNCSNSHCLKLYCQCFHQNKQCTELCKCVDCKNYDCHLQVRQTALEKIKIKSQRQKHDNDLFDLSKVWGCKCQKSKCQKNYCECYIRNQKCTSSCKCKDCANKKRIPIQLKKKKKIETVSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XU04</accession>
    <name>A0A8S1XU04_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1390009</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000139">
        <property type="protein sequence ID" value="CAD8204851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="311595at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035764473" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="1374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1129"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1158"/>
            <end position="1180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8204851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1374" mass="161179" checksum="41886AD2990D116D" modified="2022-10-12" version="1">MIIMLLVMKVACIFPNTLFSIDQEYSLYPTVGEIYEYYLEKFISETQLMCKTQTQVPNVQVINQCEEIYSTQGNQFISISSNNTHFGTLTNNNEVIIYEWKNSMIQQIGSSIKINSSFNCFNIDLSSYFSILVDCFYNNEFILIQFIDGQLIDVYHIQSSVPTQTKIQSIINETNPFIVYAQYFQNYSILTLFSSNFCNQSSLNYQFVDFDITIKINPHIYAITSQEIFQLSISPNSQFNQISNFSQDGMSNFNTINVYYNFQSYSQKFFYLLFVMTSQFGSFKGLSPSMPGSNRSVLQRPGLRDDHDAQGNHECTFTIDSYSQCDQINLIDSNGQIAPFLQCENQIIQVINDLFIYTGYSQIQKILQSNLFIIYQFIDTIIILQQQAAQYYKYTLQNQGNSLLYFNSDNELFSFNQEIIVYKISIASLQVNLTNLEIAGNNNTFSLYCKYFEQFIQFKFYLQVLAQNDTNIYVMFNQDFTQYQTSFQLSVQNSFFGFSGQLLQYQLNPQNIPLKFTLNAQQKICESSQSYQLVQFLSLYGQKYYLIGYINQSLYILQSSQICQMNLLNSINITINASSLQVAYSIYPKMIIIGLRANNAIYLFQYFDDTKNIINYQNFTFQQNFSDYLVTYNSIIILFPNQEIQILTLNFTNTFTLNQQSINILFKNILFNPQQIIVNTQSQSSIFYINNINEVIIISIDQSSIPIPISLMQINFPIKQINLVGIQLIFSYLCNNQQNICFQVWNVQNLPKYYYVKNLYSVKFLNQMIQSDNLFLYITFSNYTVYAYNPQLPYHMSLMYKLELTSPIICTQAIQGGYHYEPVYLYIYSIILLSDNSIYQLYLYQQFEISVEYYNEDFNNSISYPQFIYNYSVTSALNQTAFQQTPNQSIILYSNYTIFLNQRNLSFNLSKDYIIPNTKLISYPMNLFIDRQVEYCNQTLSKYCYLTQFSHQTNNISNFQNFYLMTQINNEFFALQNNFYLQIVNIDLNNLSNINYSYLNFSTCLQSTSYIYQLYSICENNTAQYLLNFTLNSSGTIIQLETTQLPQRVNITKISSVLNQIFILSKQQESQDSILYWLNQSNNTLQMNSISSCQDYSIALIPKVNEDIQQDKIIIFYYYNNYFIKEQIVINRLIITLIFAIYRNYAINIKYNIFKFQFYKYILTELLYFLNDIYFSYIIYLRIEKFSLYSQSNQNGTIIGTIPNYGSLQISANSFYQDGVLMQQFTQNNLSQFIVGVYYLNSLLDKNLMQPILMQGSFNTTNPNYAMIINKNYQNGTSLYFYNQSIYNYPISIWNITCILNSHTQHEINVQIFCQNEFSNGNYNITFYPPHLEQSSKSSIYALISIIRLLLLYFYIKVQHKTKNLGYIKSEIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XV40</accession>
    <name>A0A8S1XV40_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1370031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000137">
        <property type="protein sequence ID" value="CAD8204368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XV40"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8204368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="24697" checksum="C39D3CF8EB577049" modified="2022-10-12" version="1">MQFLSYLDIIDIQKLDVNISIGKIFFLQSLLCCVISTTTFLPSLQHNQLYGLFFMFILSVLNIDILGLVNQIWYSEKNIKNLWIHVNQQQQQHSLLILLSLLQKYQLQFIFQLILYLNLFTFYGLKNLIQQITKKQMGFYKCIMVLFLEHLQLLLFQCSWLQIIRKFRCEKFRNKYIYKEFLGNFRSLSNFLSLLWKQNSHELVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XXI7</accession>
    <name>A0A8S1XXI7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T1430005</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XXI7"/>
    <dbReference type="OrthoDB" id="10251371at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15845">
        <property type="entry name" value="SNARE_syntaxin16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF83">
        <property type="entry name" value="SYNTAXIN-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="10">
        <location>
            <begin position="247"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="214"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8206050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="342" mass="40061" checksum="5E3754BF4142E402" modified="2022-10-12" version="1">MANTFFSYYYQFNQFHIRMKQNLGFLKNKTHTLNSYKENLRLKTNKFKGLNQSNPLKQNSLLQNSQNNSAVSTDANISIELQSSTFGLSAEWAQDYYHTIENIRQIKELIKELQILGIKRIKMQFDDTSRIEKLIYEKNQIATTKILECEKNTQKIQKYQSPLESHSEKRIRENISIALSQQISETAGLLRNQQKRMVTMIKCISIDQNKHFLNKKEEKQIEFKENNNLTLQEEELYDQIQQDKYITINNENSDQEINKLVQMINELAQVFQSLNQLVLDQGHLLDRIDQNIDQAYKNIKKANCELKNSEESQNSPLANKCINTLIVSIFICCLLLVIKYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y3F7</accession>
    <name>A0A8S1Y3F7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trichohyalin-plectin-homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1450038</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000145">
        <property type="protein sequence ID" value="CAD8206752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y3F7"/>
    <dbReference type="OrthoDB" id="309041at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="49"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="203"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="133"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="64"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="224"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8206752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="472" mass="57130" checksum="8EDD28BF420D6FBC" modified="2022-10-12" version="1">MSEDIVNMFVSKYGSGDTNRNQLLQQSIQKVLIKEGPDSQVLTKLRQKLGQSQSQQQFFPTLGNGESSQPNKTPIDSQRKNFEPFTKRVQPFQRTYLKSFDQVSETSEKPPKSVYYVDRQDEDEWAALMKFDAQLYQKEQERKKELLQQKKAQMKKELDMQLRIKQERKQKEKDLEEKYNKFQSELLQKKNDQELEKQTSAKNKLFHLQSDRDVQLNNKKKKQHTEDKEQQNYKKEISKRYQDELQKQIDEENRIKLMKREYMFKVMKQSEADKMVIMQKQKEQEKLEQANCEKYGELLDQIEQRRIEENRKKKEKVDKNNAWSESQLLRYKLEKEDDDKLLYWKQIEEQKFLEEEQKKKNRVNELKQKTIQSLEQSIKLKQIKKLQEKELDNLQGQIWKEDTQRFFQEEQDKLDSIKKVKIEHKKILQKQMQDKIKPERGEKMSIPELLQNKTILKTIKQQDIYDLEKTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y3S2</accession>
    <name>A0A8S1Y3S2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1490073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000149">
        <property type="protein sequence ID" value="CAD8207983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y3S2"/>
    <dbReference type="OrthoDB" id="295274at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="345"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8207983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="396" mass="46108" checksum="6C7CB01A6E110C75" modified="2022-10-12" version="1">MQQPQEELDQFIQTLNNQSTIPYLQQALTQDQLLVQNARLLQQLHEQQRMLNFQSLFTQSVLQNPYINQQITHESKKIKKTKSTIQRIQSNASLKEQAPLLQEIKGNVKNAYIKKITSLLSVEGGKLLDEELQEDLQVESSSEEISKEQKFGLAGLLGSANDLLTVESKNKRLRQSAKNSRLRKKVYLKLLEKKVSDLDQKIQEYKKTTRQSFDYLTQLLSSHPILNSMIIANSNSIDQITECKKSDQAQLILDSYLMRYGTCGIKRRDYLKYAVKNIQRNFLKGNYGLQLISYNNDVKNYDDEFNNYVEIVKKQTKLDDENLIYKVLPIIDKLLNHRKTSQQLLLKFNTEMKKLKQIQQEVEENVNQFTQQLTPNQQIELIKGVEKLTTFNRSLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y4A6</accession>
    <name>A0A8S1Y4A6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1510003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000151">
        <property type="protein sequence ID" value="CAD8208450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y4A6"/>
    <dbReference type="OrthoDB" id="298777at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8208450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="535" mass="63351" checksum="EEB239D4362A4FEA" modified="2022-10-12" version="1">MHQQIQNIQVFEKSTFSNQDANLIQLEWWNPNEMKFRDVQIHITITQENNIIYSSQEGIILRKEQIYDNPNNQEILLNMEQIQHLEWLGEYGKNKRKLGKWVATWNKSLLIEGGYYVDGLKQGLWTQPFTNYFSKAKVFENGQYFHDQKYGRWHYIYKNKNIGGGSYNAQGVKYGKWLEISEKFNDDSQLTYCGEYKNGKKVGIWAVLWNDDDSWYNGEYKNKLMGGGSYDEEGDGIKQGNWLEVSDRFDNHTQVTFKGEYKNGKKVGNWHIFHRQNGKGKSIMKIGGGLYKEEEIKQGIWVELSDRFYDFSQVKYNGQYKHGKKIGRWDIFYVKEKMQKQIINGGGQYDEEGDETKLGFWVEVSDRFYCNSKVTYHGQYKNGKKIGMWNILFNWQGNKQMQQNIMQYIRFSGGGLYDKRGDGIKQGYWIEIWNGFFYQSQVTYSGQYKNDKKVGRWDILNEGDQIQEYLSLSGGGSYDEEGNEIKFGRWIELANDFWDFGQITFNGEYQDGKKVGRWVEMDLYKDEKREIMYQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y9W9</accession>
    <name>A0A8S1Y9W9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">IFT122 first beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1590057</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000159">
        <property type="protein sequence ID" value="CAD8210420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y9W9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002141">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056153">
        <property type="entry name" value="Beta-prop_IFT122_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23381">
        <property type="entry name" value="Beta-prop_IFT122_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="218"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="260"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="372"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="405"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="447"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="IFT122 first beta-propeller" evidence="2">
        <location>
            <begin position="479"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="489"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="529"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="571"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="733"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="766"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="808"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="850"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="890"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="932"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="974"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1016"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1058"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1100"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1142"/>
            <end position="1183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8210420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1267" mass="141648" checksum="41EAC53C2BFCDA10" modified="2022-10-12" version="1">MKCTFEHNKGLKDVLAILNHIDVQILSLIIEILLKEKFSDSIGFLQKYQNQKVYEQSIFKVENIQSFDKWWKLDTVQENIKKFTNVLISMKNHDFNQNDYSTKNYLQTKQLLIQEIIENKKIIQFLKFLVHLTAIDDLFIQSGSNSLHLLVEMKINLKNQFFEGIKIRNTSLIGANFFRCNLNRSGFENVDINGINLNEAQLFNCKWKKIRLNQLIKLDGHSGYVYSVCFSPDGTTLASGSEDKSIRLWDVKTGQQKAILKGNKNAVISLCFSPDGTTLVSGSADNSHHLWDVKTKKKKLKLQGPYKTIYSVCFSPDGTKLVSCGDVKDSIFSILDLDIDEIDDKDFFIFLWDVSTGKFKFLKNFAHNLALSVCFSPDSTTLASGSADNSICLWYLKTEKLKIKLKDHSDKILSLCFSPDGTTLASGSADKSICLWDLKTGQLKRKLKDHSDKVLSVCFSPDSTTLASGSADKSICLWDLKTGQLKRKLKDHNDKVLSVCFSPDGTTLASGSSDKSICFWETNLEQYKTQGHSNTVCSVCFSPDGNTLASCSDDKSIRLWEAQTGRQKSKLDGHNDTVCSVCFSFDGMTLASGGADKSIRLWDVKTGQQKAKLNGHTNLIYSVCITPDVRINLFVYGKLRQDNKNQYQITIKFFSNHFIFLLMVLHQHLVVQISLFVYGMLKQDNKKQNWMVIQIQSIQYASIDDKDFFIFLWDVSTGKFKFLKNFAHNLALSVCFSPDSTTLASGSADNSICLWYLKTEKLKIKLKDHSDKILSLCFSPDGTTLASGSADKSICLWDLKTGQLKRKLKDHSDKVLSVCFSPDSTTLASGSADKSICLWDLKTGQLKRKLKDHNDKVLSVCFSPDGTTLASGSSDKSICFWETNLEQYKTQGHSNTVCSVCFSPDGNTLASCSDDKSIRLWEAQTGRQKSKLDGHNDTVCSVCFSFDGMTLASGGADKSIRLWDVKTGQQKAKLNGHTNLIYSVCITPDGNTLASSSADKSIRLWEIKTGQQKLILDNHQIFFQSLYFSPDGTTLASGGADKSIRLWDVKTGQQKAKLDGHTNLIYSVCISPDCTTLASGSADKSICLWDVKAGQQKAKLIGHKDDVLSVCFSPDGTTLASGSADKSIRLWDVKTGQLKAKLKGHFNYVESVCFSPDGMTLASGSADNSIRLWEIKTAKEIEISENHYQNILLQFKTPLVQNNPLAHSVTTYPTILLISEQLSFQAKEALVFNGEFVNYKGYDFLSILKQKECCILQDQLQLKYKCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YBN6</accession>
    <name>A0A8S1YBN6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Carboxypeptidase</fullName>
            <ecNumber evidence="6">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1610010</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="6"/>
    <dbReference type="EMBL" id="CAJJDO010000161">
        <property type="protein sequence ID" value="CAD8210813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YBN6"/>
    <dbReference type="OrthoDB" id="433625at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000408">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF3">
        <property type="entry name" value="RETINOID-INDUCIBLE SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035856959" description="Carboxypeptidase" evidence="7">
        <location>
            <begin position="16"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8210813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="469" mass="52896" checksum="3FD6A30035B00699" modified="2022-10-12" version="1">MQKFILLTFITLGLCRLSFKEGLYNYSDIFKSEGTFPQGTKCKKVDAQKLDPLFDDTQVIYSGYLSVYENSNSALGFIFYGSQKAKQESELQNYPTLIWLNGGPGSSSQLGNFMELGPLLMQSDGNLKKNLYAWNTEYNVIFVDQPIGTGLAYVEKQSEVPTNQKQIGQQFLYALNQFLFGAKGCVEQQQVRGLTQSPWFVFGESYAGKYVPTIAKAILDYNLNAQKKIPLKGVGIGDPFTDPYAVIAEYAAYSFNLGLIDIQERMQVDSILAYGMTELNKGNTLAARDAFEESLDIIITQSGGMNVYNVLQYGNYGVIEKKVEDYLNNIQIVNKFGFSEDWKYKISNADDGPVQKALKYDFMLRDVVVTVEEVVKQIPFLIYNGQNDLICSTPGTLRWVYDLKYEKIEEYRQKDFEVLKITGTERIVGYQKSAGNLELVLINNAGHLVPTDQPEASLQMVTTFVNKHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YD26</accession>
    <name>A0A8S1YD26_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1630005</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000163">
        <property type="protein sequence ID" value="CAD8211293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YD26"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00882">
        <property type="entry name" value="Ras_like_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="2">
        <location>
            <begin position="42"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="392"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="485"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="486"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8211293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="508" mass="60494" checksum="34DE699CDEF398CB" modified="2022-10-12" version="1">MDIPNKKIENIPEEKKLLEMSQIIQKLVEEFTKNTKNQGSVVLFVGSTGSGKSTTFNFLSGAEFKLNVDEELELKNILKNFSNMNPGLESVTKEPNFFYNESNKHLIIDFPGFLDTNGDWDQLLIELLFNKIVSLGELKIIYVLNNPQNTLQNRGSDLQEFLKQLDNPKINLILNCYMANWADEKLKNKIKNDLEQVKSSVNEPKLIDLIDKILVKRKASNQDQLNSIFNNEQRQIFWREIEELSKIKIKPRRIHKSEIISQYIISKATNIIQEYGDILSLMFEQNNNLEDEKYNQQQHQNLLQFQVLLKNKNKLSPLQWFFDFIKICEELTLNLYKSKSREFLKIFQYFEQFQEFIDGYQYLDNINKQAQQNLQQVEKLFNLKIESQEKIKKAEEEKRKAEEDKRRAEEDKRKAEEDKRKAEEEKLKIQQEIANQQLEAEKLKYKMLEQQREHEYRMHALEQNYKQQELDRCRSYYNVQLTKLRNTVKKKNSGKKKKNPTKQKKSCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YEN4</accession>
    <name>A0A8S1YEN4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">peptidyl-tRNA hydrolase</fullName>
            <ecNumber evidence="1">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1670049</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the PTH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000167">
        <property type="protein sequence ID" value="CAD8212250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YEN4"/>
    <dbReference type="OrthoDB" id="311283at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00462">
        <property type="entry name" value="PTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001328">
        <property type="entry name" value="Pept_tRNA_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018171">
        <property type="entry name" value="Pept_tRNA_hydro_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00447">
        <property type="entry name" value="pth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17224">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17224:SF1">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01195">
        <property type="entry name" value="Pept_tRNA_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01196">
        <property type="entry name" value="PEPT_TRNA_HYDROL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8212250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="188" mass="21006" checksum="F86489B2BEF052CF" modified="2022-10-12" version="1">MSYLIHKIRYAFSVQRIPNGTHLIVGLGNPGKIYENTRHNAGQLFIKHLAKEFDIKMNNGSNGSIGSFQHIILFNPLSFMNSSGHPIKKIADKANISTSNIIIVHDDLDNLPGRCKIKLGGSAEGHNGLKSIIQYMDDKFIRLKIGIGRPNSKDPAIVSDYVMSKLDYEPSQQAFKQGIVLVRQLFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YEZ0</accession>
    <name>A0A8S1YEZ0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1650045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000165">
        <property type="protein sequence ID" value="CAD8211798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YEZ0"/>
    <dbReference type="OrthoDB" id="444258at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8211798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="178" mass="20471" checksum="7998707C9FBFA9F4" modified="2022-10-12" version="1">MKQKIDRSRIPNSSQDILIVPVYADKLGFSLPAKLPYMPVSEDSIAETVFQANRICQKIRCEKSRIEESDPLETEKFYVTSSWVLFIVGVILFVLGFSYEDFKSTLTLLGTIFIVLPTLISIIVVIISITKSPKLIDLEQECTKKLGEFFEVQNQQYRKKGLQWSIGDEMLWIQLEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YLC0</accession>
    <name>A0A8S1YLC0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1650073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000165">
        <property type="protein sequence ID" value="CAD8211854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YLC0"/>
    <dbReference type="OrthoDB" id="10338957at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="65"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8211854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="151" mass="18198" checksum="D1A19C1E466B89CC" modified="2022-10-12" version="1">MIKNKQLITYFNKSSIQKRAKSSLNYVVSVKQHNVEQSHNNSIDSLNTRVMEVFEQQQKDDQVQIQRLLIKKQLLEIRIRHENEKYEETLKNYEQLLQRLESMEHSQKEYISQMNQLTDCLREIVQQNNEMESAYDDKCKEYDSLIQIINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YQ35</accession>
    <name>A0A8S1YQ35_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1940005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000194">
        <property type="protein sequence ID" value="CAD8214022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YQ35"/>
    <dbReference type="OrthoDB" id="317435at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8214022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="127" mass="15181" checksum="0709C7260F0C791C" modified="2022-10-12" version="1">MKQNSQFQQKQDLQYIDHPDTPALFWCKSNDCDENRIFCLSCQKQNKHNQHYNEDVLCIHELNQFLIDQSKQHENLISECQLQQKQTIKSLDKLIYGLSYKFCGIEDKINQFKNYQTQQALDSFIKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RX39</accession>
    <name>A0A8S1RX39_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0010398</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2162994at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8132012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1382" mass="162983" checksum="0007FC6BA4FCEEDD" modified="2022-10-12" version="1">MLQGDVPSEVQLAIPCIQLKSQSVRLIANMQKCEIDCLASYELTIGFSDSKDRLVDLNQTMEFILYGRQYEIKNITYIPSEKYPQIIKLVKQQFNSNEKQSNLLKSQKPFKSPCSYMTMNALLNKIQDDCQQITLLFQFATEKLESTDYDYIKSLEDQIVFKLQISYVISRPISGLRFINNLDNKFLITEKGLHNSRNWIPTPYYKQQRYPPYCHEEKDLLHYSKIIITVPSSYYVVASGQLQEIVEIDGNEQVGYVFNVNEYINPDDLGIFIGEFSHHYANHDEFRLNIFHMNENKSYLFESETARKIFESVDRFWFRDALLQKARNILSIGSGVEFFRSLNVLILPNVQKHGYLSFYPFRNILVFDESQLPETLPCKHTQDEFYRCLHLQLNALSLKFFLAPSVKLSDRWIELGFTFQSTWTEDYAQLHQKLLEQIKNGQDVKLDQTQFEQRFLLESLEYQLKTRMVITMLARMTHQPDVFIKEILNRFYQDLKGSIPAPLSTDKIFMSMKKVFSVSKGKSFLKMFIKSAGAWHFKVSYDYERDHKLNLNITQSPLIGHWVHVYTHNYIQGAKALDIKSDIIFQFLQFQEKIKHTQKQIQKAFKDFEEQQKEQPYDQSKKPHSSSTIKDFKRRDYQIQASGLIPEELSMPLHFSGWVRIQVSETNELKLEIENTEIKIPPKLWTKIDINCTQKLKKYNIPKRHLEINAQAYIQEEQTGKIEIENDNNNQVKTPMIHGILGEAVLKSGVNINTGREQTIKSYSEEIQKEPPQWIKWDPVGLTVKEVEYQIQKELVVMCQVLKEKDMNQIACVLRNIKKLEIRERVVRTIVLIIENSYAHLESNIIVQLLQLLREMVNKSSIKRLEENCDFEDMAELQAYLKLAEKITYRFFFNQNDNSLKYLKFNNFDDYDVIIALLKNLRRLNTASTLGFHNELIALLVSILQGYDNSQNQYDCSYMISKLIKLLLFTNCNQSKFLIMTTLKHELKKVIFLNTSKNYILKTIIIHYKRFTDINFSGEMEKKIEEIYKKYDEEISSIIKHHQSNVLVRIDNGYLERAYYKKYLLEQYEKQKKEPYHFILDQLYYLSKKRVKNGSTTIYFNVLLQELQIFMAKNYQRFAHELSRADINPELAKTLAHTNFECIISGPALLNYNVQSNFASIYRILFYYFAPLRTDEVHKDIKHLLYTLTNFTPDQQWSQAHATQLLEEYQELTKRTCGQQPKKNRPVNRSTNQDLIQQLERFARKFPNKLSEREITKQILKHLGTLPQIGQLEEKVGELSPSSKQIYRIDQVRASMKRKNQPMNIILDNVLSMFEYYYRKQQLSKNELDDFCQMVRKFFDDGKKVLENYSKANVDKKVQKMVIKSTSLETNLAKNTYDEMNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S1U8</accession>
    <name>A0A8S1S1U8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0020114</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8132874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S1U8"/>
    <dbReference type="OrthoDB" id="308048at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37473">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37473:SF1">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="271"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="22"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="162"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="280"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="299"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8132874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="309" mass="37056" checksum="2E33546E4057AA31" modified="2022-10-12" version="1">MLPRISQSQQLHSLHYSEEVTLNEVKRRKKELELQAQSVENRIRQLRNEEGKLTKRIILTEQLTSDVYKQKVAAQLKKQQKIQKNVIYNRNAQNDSKQLTEQSIGNERNKELYIKTTIERNQLKKVKQEFRQYKHDEAQNFRKFLKYEQFKFIEEQTKWHENQKLKAALRKQEQNLSEVLIREKLNEKRYKIKQEMEKEKVKTIQENEEKEKHIKQLEIEELQMVHKLQATQQKEKDVKGKLIAATRLQPDQLAQAFHSLNNTTIIVEQSNIQNNKQEDNVQQNQQSQNNNEQKEEQTNEGQQGTQTQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S6H9</accession>
    <name>A0A8S1S6H9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0040052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S6H9"/>
    <dbReference type="OrthoDB" id="296597at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8135097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="257" mass="29963" checksum="3DA45ED81841433A" modified="2022-10-12" version="1">MGNNCSYKQQMSDKTQFDQLNSKSNLNRMSKPKLDSLINTSQLTQMEIPNAMLQSPTKPFIPNQNEIKSLVRVPSEESLFLSQFNIEKENEQENNSINQQKQKSKPLMALKSSLKRKNKQNQVQTPNFQSPSPKEPQQIKCRSHSPFYSSAKVEIKKKKSIEYSKQITLQQDSKPRRKYSDAPIQKEKPKLMRRKISDIRDDHNYIIGIESYSPTKLRTHTPTSILKRKDSDIETNSPMKKQVRFKEQGINRKLHNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S835</accession>
    <name>A0A8S1S835_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GB1/RHD3-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0050134</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S835"/>
    <dbReference type="OrthoDB" id="2135133at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01851">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001470">
        <property type="entry name" value="Interferon-induced guanylate-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030386">
        <property type="entry name" value="G_GB1_RHD3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015894">
        <property type="entry name" value="Guanylate-bd_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10751">
        <property type="entry name" value="GUANYLATE BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02263">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51715">
        <property type="entry name" value="G_GB1_RHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GB1/RHD3-type G" evidence="5">
        <location>
            <begin position="39"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="511"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="667"/>
            <end position="778"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8136406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="833" mass="98210" checksum="0479846EC3BD0831" modified="2022-10-12" version="1">MQGQPIPFIELKETQTKEGLETIFEIHPQAIKMLQSIADRKVAVLTIAGPQRTGKSFLANRVLKRQKGFAVGPTTMPCTKGIWLWSQPIPLNENTSILLMDTEGLNSVQRDLGVDTKIFSISLLLSSMFVFNQLGHIDEQSIENLSLVIKLSENISVGSEDKSLSQFFPTFLWVLRDFSLDLRGRTPSEYLEFALQDQSNPGPDGERKNLIRRKIREYFKFRDCICMVRPIQDERRLARVEEEDWNALRPEFIAAVYDFERRVSKNIPPKQINGITLTAEMFLKLSLEYVDAINSGGIPQILSSLDRVIQQEARNLLEELKSQFSKSYQDALSKLKAPFEDEELIQLNKQIQAEIFQRLDQKSKEIIDNSKIISMKKELHDMMSQDLQTRLEINKEQSTTVANQILQKFFNTFQVPVLDNVDSIKSSLLVDQFQIYSRFFKYYMENVKGHQKYKHFSEKVPSFLFSHFEKLILNVQKVYIEENNQTKKYLVQAREGEERLRKTIANQESLLYEIQKERDQLKYEIENWSRETNKQEKIKSLELEACQAKVMQLMQELQNKKEKNQKLKLDFQNLQDQLQRVRNELNGKRVECQQLLKRMSDDNQSFREPGEEISNDLVLQYKDMKQQAHQMNDFFKRRAMDHSQQSIFIQEISKLQQDKFNEIIKLREDYKQKKLKMKEDLEQEKKSLKNNLEHFIKQNEELKAKNMTYYEKYEQAQKNQQHLKQIQSENQRLGQRLEEKSENLDMHIQVIENFTKDIEKLTGIREDLEQKLAFMNSEKSQLISFKESFPYILKEALKWVLKQNSKIKHAMKSVGEEEQKIIRDCFHEVGINV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SA89</accession>
    <name>A0A8S1SA89_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Kinase domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0050441</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SA89"/>
    <dbReference type="OrthoDB" id="302647at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8136978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="325" mass="37558" checksum="5258B156145E30D9" modified="2022-10-12" version="1">MSKNHNFEQVCQDICIYHKSVEHILKPNQKPNSKLNLKKDKLMKKFASQIIRSEQRIKSPFQIEIFQQQQQLNDKCVEKMSSELVLSQPQKNLSIDLSKKWHLDLSDLTDKGVRDLSNSLSKLQSLEQLNLALCGWGYWNQNITDKSLQYITNALILQNQLHEFNLDLNMWAYENNSITDEGAKNLMDGISLLSNLGKLELNLKGWGDGNQDITDNTILGLSRCLKKLGNLSEVKLVLWQNIGKQAIKQLNKTLSKMENLTKIEVKFESCAQQQPVQNNNIDQKLQQIKINAVQKRRLLFQVEGFMINLESIINQRTLWDIILKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SCL0</accession>
    <name>A0A8S1SCL0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0060052</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SCL0"/>
    <dbReference type="OrthoDB" id="299978at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8137360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1141" mass="135526" checksum="6793963BBE70BB13" modified="2022-10-12" version="1">MSQQEIECSTDCKAIGLTNEKKEKIQNRITIDQMPNKGLPNCHCQLSKDHKSKKCQSMAKCKYQLTNELSQLIDITLYKYLNEISNSDTSIEATAYKKIIHCIRDFNSMKDNNDEFRLKLKLINKDQEIEAQDKDQEPLEESYPKLAAELKESPQDFKNGHFIRIAICLQEQIDQILDGEETFFFFFFKQGLDCSIQKSIQFLQRANQREIECQWDQSQGSTSVSKLKSDTYKISLISIQKNQLDFEPLKKENLQFLNFYQDECQGQIYNKINTSNINYNIPIENSLESLKVALKFFLKEKLKEFKPHIIIFFFEISNTLIKDPQIFYQLVKSFHKISQGRLIIIPQIDQQFQFSKSEIIQLYINCTNNCFLGCLKNQHTQKLDQSSLIQYYERLYPYFIEANNQLKKQYYRNQLKSLQIQQQKQVEKQSRQNLHKEFPLIDKDGNLVLLDIGQEIDITKIQYFVEYDQNQNGEYNGFIILIENKRIQVIFFSEQNPLVQSKNQKFSIVKKQQISLPQNKNGVYFFDKELNILIYFFGYSETDEFCSDIQFIEISNQIIEWQIVKYQQSKKKVEDGTYTNFQQIINLIKYRSQFSVTKNRIDGFSSQDNSYVCIGGTYYNDECNYGCIIQEVVIEFKQKVYNTKIVEKSKIDKFYKECPNPLLIRMNEAFVLYFEKNGPFNINNCNPSITKLCFWKSRQHLIQNIKINFKDNKTSHWLLQLNQQLSSNQKNIRMCHVQQAVENLLSFRILVEEQFVQKQPLYNENKLTLRKFFILTISFFSLKNEILIDCKQFQMNSHIQLKTINDLQREEKQELYQNSVENLKNTFIFHYKEYDHQYILCYINETKKVEFKRFVFYDPKTQDNDDFRDYQELIYQDWAFSKLYLIRKQITLTECQLRIYECHHINFIDIKDSQDDILHQVHIHQIYMISDFKSTYQLLGCHVEIKNDDTFEAIVLTNKNYYQIVNQKKAITHTNKKHNKPLNVNAQQKFYFIKFKSSYYLFYKKLQFQQASVNKVSQNIIEIKYLFLDSNLQVLIGNTILCSLNENLPIFGPLDGLINTIKSQDNLMEIQILINQNTLIFKSSIICRVNQETNQLDQLNVNSQIPSKNEGFNMFPLIQSNRKKGDYIVKQSKDHSKICCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SH20</accession>
    <name>A0A8S1SH20_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeodomain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0060205</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SH20"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019185">
        <property type="term" value="C:snRNA-activating protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001006">
        <property type="term" value="F:RNA polymerase III type 3 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042796">
        <property type="term" value="P:snRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051575">
        <property type="entry name" value="Myb-like_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46621">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46621:SF1">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="112"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="172"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="172"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="377"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8137664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="503" mass="60478" checksum="706AD8CA40491A9F" modified="2022-10-12" version="1">MQNFHDFLNKLRNYQEIDIPCYYRNQFKSKESLIINSNFYEISEKLLTQDYHKINKKWDQQCKQLFILIIIKYFQMKNKKTINPTSEEWKELTCIFGFEEQTLKQRWITLINPMAKSLIWEKEEDDIIKSQMNVKQGKHIWTEIALELYNHNNGIYVRTPKQVRERWMNYLNPILKKSSWTDREDNQLLSLVIENGKKWSMISKYLDGRTENQVKNRFKSLLHKIYQEQDDDDIEELIALKQYISKRLIKSPDQKIQQHTNIIETTTDKNQKIVTQKQKKLEKDDDIPNQLKKTKIDIEKHEQKNALNQENQYIYLLQQVNNQFCYSTQGNCDTQSLGNRSSLSDLNYLGSFKFPEIDNNCYEQNKISTKIFPDSLKILEQQQLNQFNNKIKQQNNELESNNISQSNIKDINVQEFYIDQFPNLFEQNQSASIIHTPLYFYNPQFIAKSPLLSPLQSPSLWMHQQEHILEQDNQDNQFQFLNNYDLISKWKNKRIYDKLNKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJK1</accession>
    <name>A0A8S1SJK1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0080221</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJK1"/>
    <dbReference type="OrthoDB" id="5590282at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000089">
        <property type="entry name" value="Cyclin, N-terminal domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000183">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0195">Cyclin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="2">
        <location>
            <begin position="162"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="2">
        <location>
            <begin position="271"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8139646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="387" mass="45530" checksum="7F9FA15FE3D425B7" modified="2022-10-12" version="1">MEIKYNRSLSGVPENQQQQPLRRITKTYIENRDPVRRNLTMLYNEDTQQINKSMLSSNNDLRRKMLNQDRVKRSLNQDVLEDKENVPQHKGITTKLYTLPQELQNITIKPKFDHTIQENKNLQKYYTDEIFFYLKEQEKKSTPQEFLKIHSIPSNLRAKMIDWMVEVLCSYKCTDQTFFVAVRTLDFYFAKNEKQLEVSDLHLCGVTSMFIAAKYEEIHPMKLSVVYEKIAHKKLTTDQIKKKESEILQTIGFDLVGGTLFDLYNLILTNCFIESKLLEKNYKYLKKLCLYLAKMVLYDYEICGKNNYTLLSAALIFVAFKIVEQLETTFNADSQIKDVADIIQVDQDQLVEVAAKILNLAKNFEKHFPNLENLKKFNGFQLEDDEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SNR8</accession>
    <name>A0A8S1SNR8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0110108</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SNR8"/>
    <dbReference type="OrthoDB" id="10281635at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8142375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="279" mass="32592" checksum="46A5C8D5DE422757" modified="2022-10-12" version="1">MQKTTLVNFLHNGSNFYPFGDKPEKLNSTEGRFVPFIFERNQVGKLANAEIYNLNSNQKRIKQRTSEQTNSLLADTIIKSKLHTEQDTPKYDPITKYMTLIPTHHQIDDLHKTIDIKSYTLNHTSVERGLQMRKQQKGLNHKHQLISRSTKTLASQIPAIGIYNPIPINRHLPNIQIKSKSIQKLPEIWEHTTQVTTPKINIKNSESEMMFAKQIAQINREVDSEMKRNPNQKFSTLQFDVHENMNLEEAEQMRNRMKQYFQSVKDSLTKIKNLINVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SPI7</accession>
    <name>A0A8S1SPI7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0100027</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SPI7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8141269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="728" mass="85336" checksum="451193C06B0EDF39" modified="2022-10-12" version="1">MIKQEQFIWEEGTQELKGIYWDYRYNPPLQRVTKLQIIFTKHKEIQYFRDGSRLRIEQIKDTSKKPEILNNIEQILYLQWSGKYKQNIQKIGKWIAKWNGQIIQDAGGYYSDKGNKTGFWKELYINYWTEAQVYEEGEYNEGLRTGYWKQIFNAQLMGHGQYNIKGQKCGKWVELWEGFWRKSQVKQVGEYKNGKKVGIWDFEYRDSKIGGGSYEEGGDGVKIGRWIDLDDTYDNSTLVTHIGVYKNGKKVGLWKTFEKQSLCNIIYIGEFEKGKKVGIWDIYWIWNGNQIIGYSGGGAYDDKGNGLKIGKWIDLDDEFNYYYQVIHKGEYKNGHKVGRWEMQYKDDTHRQFSTFGGGSYDQGSDGIKVGKWVELDQKLDKSSQVIHCGEYKNGKKVGRWEILQKEDINQGYSTIGGGSYNEVGEEFKIGKWIDLDDNFENSKQVTYNGEYKNSKKVGKWDIKYYNEQIGGGQYDDQGDGAKIGKWIDLDSQFNDSYQVTHDGEYKNGKKVGRWDIKYRDEKSKVFATMYKILYTVNYSGGGQYHEGKDGTKIGKWIDLDDKFENSKQVTYNGEYKNGKKVGKWDIEYRDNSSKQFSKIGGGQYDQGSDEIKIGKWIDLDERFYLYNQVTYNGEYKNGKKVGRWDIEFRDNSSQLFYKIGGGSYDDRGDGVKIGKWIELDEGFYLYKQVIHNGDYNNGKKVGKWMEMKREWKLMKDGFKKVNEQNYEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SV13</accession>
    <name>A0A8S1SV13_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0110143</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SV13"/>
    <dbReference type="OrthoDB" id="304064at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8142444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="309" mass="37573" checksum="F9E7FA213CCFE6C0" modified="2022-10-12" version="1">MLPIVYATSYINYPQLNLQQVYPIPTQYYNIFPTKVTCDQACQFPEITLNVEASENTDSNSLTKCKEALVLEELNLLLRFLSQNISLLQEKTFEKFVIENLKNLISLHCDIPNILKRRYIQVNKTKEEMTKFIIRRCFDFIKTQINYQEKDNLGAEERDRLFYHTFFSNDKEFMKILGNDSIDDLIPFRKESKLKTINDTYLKKLFKSKYFSYFYQQFLNEFQTICQQENEEKIEKMTMQIQKIIFTRDYDKIKTYRRFPWKNHEFHKCEKRAQEIYQKYQFYKFKNEKKLLKKESIKLEHIELSESIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SV79</accession>
    <name>A0A8S1SV79_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0110220</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SV79"/>
    <dbReference type="OrthoDB" id="31113at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16171">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-G CELLS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="44"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="76"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8142554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="384" mass="44935" checksum="66E125CBBB4226B9" modified="2022-10-12" version="1">MNSNQTDLHEQQVVENTENIDTTNTEQLLDFTNMFKTQKVAKPKLLNRQQQQQQQQQQQSQQQQSQSTQSTEEQNNKSVQNIVPRNLTITQEKMSQRRCLDVRRWYSLSRPQYQRSCGISSVVTCWNYLFSTLGVGNLNPLSQEEVIVSLGLTTEVGPHFNDVEFGSFSGNMSLISWFKNLCRLHKVQGRAYFLWKNEGDFATPGVDRDVALQKLTAGLKTDKVSFIYHAYDHYFCPIGYECTPNKQVEAFVDEIDYNDCQYWIIIAEPAKPYPMFTVRKWVDIAQDLELKYPQYMNIRKMDEGIKNYKYEKGVSQHCIMAFERIDPKPKKIIEKKEKPIINEEVHLEKTNQLDQKVETNEQIHQNTEILDPLINNQLKQNDQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SXE2</accession>
    <name>A0A8S1SXE2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0120464</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SXE2"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000654">
        <property type="entry name" value="Protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="17"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="329"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="398"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="329"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="342"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8143907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="612" mass="71026" checksum="6FBD2A9956267A15" modified="2022-10-12" version="1">MQHLSHAPDKKIQNYQFSFKSKLGKGAYGTVYAGQNTNDNSIVALKIIDKKLLLTDYANQLIVSEIEIMKKINDSHVVKLLDVLQSANNTYIITEFCNGGDLREFIKARKVIPEDEALKILKDLLQGIKALLKQGIIHRDIKPANILKHDSQFKITDFGFAKQIDQNLDTIMCSLVGTPLYMSPQILKRGKYSSKCDIWSLGLILYEMLYGMTPWHSQNLVELMNKLDSKPLQFPVHPYISDSTKLLIKGCLQINEDKRWNWDDLFKAVYLNSNENKENSPISIIKDDLRSPTYRQDQNSQKNDYKYSLQMQRIKQRTESLCQQINKHNRSYSNATFQTNDKSNRQDNKTPVNDKQKLKQNNIQQERERSTSQNYRFHTQRNELTTFFDKIQNAQQKLNSNNDSCRTASALQSNSTNTSNKVNYDLDLVKVSDFQLEDNNRVSDYNTTYRNPKTERVKRTNSLSGHFQNQSFQVKPQLQQLIKIIENQLDLIPNYINCKIEINTMVILHKEIIYCATNKQVYTSKDLKDLKLLINSLISYLNENTGENSSKQSQIMLLLLLTYHKVLIYNIKNKSLNIDANLIETIKKSIQVQQNQINFESKLLRELINLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T050</accession>
    <name>A0A8S1T050_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mediator of RNA polymerase II transcription subunit 31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0150064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mediator complex subunit 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8145880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T050"/>
    <dbReference type="OrthoDB" id="10257739at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008831">
        <property type="entry name" value="Mediator_Med31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13186">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05669">
        <property type="entry name" value="Med31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0010">Activator</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU364129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8145880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="104" mass="13097" checksum="D2811C319BF3E143" modified="2022-10-12" version="1">MQKIYDDYKRFQMDLEFVQMLASPSYIHQLAIKDYFQDDQFINYLEYLQYFKRPEFMKYIRYPVCIKMLDCLQNEEFRRQMKNWNFADKITQQIEVTFQILKDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T4N4</accession>
    <name>A0A8S1T4N4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0180039</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T4N4"/>
    <dbReference type="OrthoDB" id="298868at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8148385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="169" mass="20456" checksum="BFFE2ABFE76E737F" modified="2022-10-12" version="1">MKANRFIIHNPTIESYQLEEYYNHFKRMEEIDKDKINIYKTIEQNKSATNSRFLMLQHTSKFNKQQQQNAQDKLQKLLDDKIERIKVRENPYVNLNRFRGISQTDLKSPNNTFNSSLENMNLIKKLQTLKPNISKKNLDIHYEKQREYGNRLRKLPKLPDLDKKIHYLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T4T0</accession>
    <name>A0A8S1T4T0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0150416</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T4T0"/>
    <dbReference type="OrthoDB" id="191686at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051480">
        <property type="term" value="P:regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051001">
        <property type="entry name" value="Calbindin_Ca-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19972">
        <property type="entry name" value="CALBINDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19972:SF10">
        <property type="entry name" value="CALBINDIN-32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="177"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8146579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="260" mass="29775" checksum="2A90197800DCDF89" modified="2022-10-12" version="1">MDHNRDIKYSTLKVSDSVQFGGIMTKEECQALAKKIMSIYDGDGNGTLDSFEVGYIQSDCYRAMNKGFNPTPTDIAAFSRIIDRRQCGMVTDKDIEALCIKYFGGNFDRSERSERVVPEQTISQQRTYETSSFNRQVSSNQNSKVITVQKQTIVTNQVQQIPSSPPVKNTYSQVVQERLEVARRIFRMLDTEKNGFITDKHVPSLLQETYKLMGMSIEPTQEDVELWMEMADEDRDGKVYLKDYEALVIRSLKQQGIVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TA76</accession>
    <name>A0A8S1TA76_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ligand-effect modulator 3 LEM3 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0160411</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the CDC50/LEM3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TA76"/>
    <dbReference type="OrthoDB" id="340608at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005045">
        <property type="entry name" value="CDC50/LEM3_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10926:SF0">
        <property type="entry name" value="CDC50, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10926">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03381">
        <property type="entry name" value="CDC50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015840">
        <property type="entry name" value="DUF284_TM_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR015840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8147462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="307" mass="35164" checksum="238A3DF90F961E03" modified="2022-10-12" version="1">MQAQPKGFSYAFKQQIMKAWQPVPTLNSTIILFATMSLFFLIMGIVLISYSNDIATQEFQYDSYCSAQNSDCVIQIALDSNYKSPVFFYYQLTNFYQNHRRYVKSKSPSQLSGTDLSESELDECDPVVTNDEMGKTSSVTNEPLKKEEKAIPCGLIAKSYFNDTFNLFQVINNTKTEIKISSNGIAWPSDLDGRYKNIDLNRQWIDMEDERFMVWMRTAALPQFRKLWGKIDQDLEAGIYEVQIKNTYNVSSFNGKKYIVFSTTNAFGGKNEFLSIAYICVGVVCCVVTLGFLIRKFLSKADQKKNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TBB8</accession>
    <name>A0A8S1TBB8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PiggyBac transposable element-derived protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0180307</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TBB8"/>
    <dbReference type="OrthoDB" id="302366at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029526">
        <property type="entry name" value="PGBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46599">
        <property type="entry name" value="PIGGYBAC TRANSPOSABLE ELEMENT-DERIVED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46599:SF3">
        <property type="entry name" value="PIGGYBAC TRANSPOSABLE ELEMENT-DERIVED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13843">
        <property type="entry name" value="DDE_Tnp_1_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PiggyBac transposable element-derived protein" evidence="2">
        <location>
            <begin position="96"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="686"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="686"/>
            <end position="713"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8148917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="736" mass="87816" checksum="1BDA980C35A3EB0C" modified="2022-10-12" version="1">MKQEALSNSVAQISGNSQQTQILDEIEKYNCYWKQEIILSDCEEQFVQIASDDERDCAAMINFGKALNIQQTDITQITEILNYLDTPQLKMTSLQSPQNVLNYFLPSSFVSTLTEMINHYLLMVLQEDLKQQQQQTQQIYHKKKYKEAEIQFYLGLQILFGVYRFPSLDDYWNAESWLKGGVEVTMPIGRFKFVDLHIFSYFDELQRAKLQLEVRKFSKKLKALYNPDQELIIVEQNQKAYKTYYIFDYDSSQIIDLLVVCNNIKNEDRINRVMRMLYKYSNQNHVTYIMFDLSLERIIQLVDQSIYPVIRYQNINHNLIQMLQDGDYELDQLFLIKQGTQVDIFPQRRLISNRHYMTSYEKIKEQLQEHIINLQQFRSNFYQSSQLNNTEGFHQLRVEFEEYFEIMIHNTFLLIQQTSQSQFRHDLAKVLLNEKGQQIERDRNEAKRKLNVTYKENYSNTDHLSFGLQKQYADQFHTPIPQKQEDTFKYCLVCMKFDGLTKPYYRCQLCEKLLNVSKIFLCPFPCFELFHRNPSDFIDCDMKMLEPLGDGIYFKNESTMQLVNNGQEEDASGKKRKYNKPPPRTYWSFNKEIYRQANINDDQNLNDILNTFKKPQAPAPSPSLLQSIVPQEQQQQTLTLGHKKDMKDIDHLLNQEKEQKDKLIKEMQERDKQLQEQYFQQQYEKQLQKEQETKKSKKAQKESEKKKQLEEQLKNVSPLEKFMEQINKRAKVEDIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TCX7</accession>
    <name>A0A8S1TCX7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0210149</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TCX7"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000334">
        <property type="entry name" value="Heat shock factor protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF206">
        <property type="entry name" value="HSF-TYPE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00434">
        <property type="entry name" value="HSF_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="3">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8151055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="246" mass="29165" checksum="A1C4C7F5879E250E" modified="2022-10-12" version="1">MSTRTPIPTFILKTYQMLEEPKNSNIVSWTAQGTAFIVYNQQQMEKQVLQNFFKHSNYSSFVRQLNLYNFKKVRSNEGQIFKHKCFRKGMKSMLQFIKRRNQEEIQAPQTQEEPTINIKEEQNLFKECALDIKETNNKLKEDMKLLQETSSYLIDQMQNLNHFVYNQSVDIEVKFKQVGQMLHAINEELRQENKSETQTNKLLGDYKYTKELDFQESKIGSPNPYVDYNSTALNPLDYECFIDSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TD13</accession>
    <name>A0A8S1TD13_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0180448</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8149166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TD13"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8149166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="79" mass="9382" checksum="F83A7B84C4B11C98" modified="2022-10-12" version="1">MSLIHKTSKELNETSYKKNKLIGIILMKKYQNRETKNSQYKNNYSPNILDNSTLILLTEKCQIESIMKAPYIQSMQKYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TEC6</accession>
    <name>A0A8S1TEC6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Calcium-dependent protein kinase 1</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="ORF">PPENT_87.1.T0190021</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cell membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Parasitophorous vacuole membrane</location>
            <topology evidence="24">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="30">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TEC6"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020002">
        <property type="term" value="C:host cell plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020005">
        <property type="term" value="C:symbiont-containing vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000398">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000199">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000695">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 26" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17" id="KW-0969">Cilium</keyword>
    <keyword evidence="15" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1032">Host cell membrane</keyword>
    <keyword evidence="16" id="KW-1043">Host membrane</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0519">Myristate</keyword>
    <keyword evidence="12 26" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0564">Palmitate</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="28">
        <location>
            <begin position="134"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="432"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="470"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="506"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="545"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="24"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="27">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00060437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00068067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="EMBL" id="CAD8149262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="572" mass="66118" checksum="872D26C45864B68D" modified="2022-10-12" version="1">MGSQCCKNNNIRCQEEVILQNEQTANASPNPLQKVSLSQQGSKKSQIEEDQILVESQQPGYVLESENNQEVDQQEEMPKKEIEVIKKEESVPIMKTDSKEEIKKKIEPQVPGVRIKLGHDIFVKLKEGSIGSHYNFGKVLGQGAFGKVWKVTHKTTGLVRAIKQLKKSSLIKEDEQRMFSEMNILKNLDHPHIVKLFELYQDENNYYLVTEYLSGGELFDRIKKMSSFSENIAADYIRQILLATMHCHQQNIVHRDLKPENVIFISEDPNSQLKVIDFGTSRKFDNTKAMSKRLGTPYYIAPEVLNHQYNEKCDIWSCGIILYILLCGYPPFSGKSENQILERVKLGKFTFDPEDWDQISKEAKEFITKLLRMDPNKRLSAKQALDDPWLVKYAPTSQVNKRVLDNIRQFRAQTILKQALMSYMITQMSTQKEISELQNEFKRLDINNDGFLSKEELIKGYQQLKLDYKYAQEEVDRMIDMIDINRSGMIDFSEFCMAAINQEKLLSVQRVEQAFKIFDLNGDGFISKQELESIMGDLGDDVWSQILSDCDGNNDGKISYEEFVRMLKNKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TFM9</accession>
    <name>A0A8S1TFM9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0200263</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TFM9"/>
    <dbReference type="OrthoDB" id="309842at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="452"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="462"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8150444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="509" mass="60941" checksum="C33E36B54650F728" modified="2022-10-12" version="1">MLREGPLQRCPMCGQVFKLVRLRQQEDEEMSYYRDSFHPIDIFELDNENVQSINMLKMWTHREASLFESALYEQNIMVTMDNDVHDRLLVDPAYRMWRFQHGEDKLKYIDQQLKQAGYNEERVYRNPKYKLPMQKATYAAVIEAEKVLAIQERLERKVNRFHVREYLDFANHARRERRMRLRQKERWENNYTYFYGGLTEEEQLYNDYFETDQELYKDDEQVEQRIDEAVAQVDPKYSPLRFDFQEAYTHNPEEDQTSLLEKKLWRFKYRLAFDCTKDYQVRERRLIERHLARMTKDPEYIAVFKNLQTAVNSENEFAILQAEKNYYNLAAKEGFLQYKDYFEGDSSAEVELVEELYKASPLAFSKVFVNHSARLFQQEGFQKFPKRAWNDSLGLIQNYVLDFQDFSSNIVPKAQKLANYAGLQNVLPADEQELVKLGLDKTEQIARQKIAQAQEKPQLNEPPKQQAQQQQAPPKVETAQQQQKPQQQQQQQQQQQQQQQKKGPFNKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TJH8</accession>
    <name>A0A8S1TJH8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Actin-related protein 2/3 complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0220255</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8152053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TJH8"/>
    <dbReference type="OrthoDB" id="200404at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007204">
        <property type="entry name" value="ARPC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12391">
        <property type="entry name" value="ARP2/3 COMPLEX 21 KD SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04062">
        <property type="entry name" value="P21-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8152053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="173" mass="19757" checksum="61795FC3D67B3223" modified="2022-10-12" version="1">MQQTVVTIDPSVCHAKLKSDKTLCGLAYFDDQKSQRGIVLESLEYFRLMTFFKNYNYEGPSDILLIYLWVVCAHMIKIIENDQDQQVAAKKLDEIAREAIPTRSGDRKNFLGGLLKETPGETDKIQAYLKGLKDILAKELIAKFFKDGKRTFDAKFWSGIAKRKFMGLSYTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TP50</accession>
    <name>A0A8S1TP50_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0270299</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TP50"/>
    <dbReference type="OrthoDB" id="1705416at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002838">
        <property type="entry name" value="AIM24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43657:SF1">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 24, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43657">
        <property type="entry name" value="TRYPTOPHAN RNA-BINDING ATTENUATOR PROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01987">
        <property type="entry name" value="AIM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8155801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="235" mass="26802" checksum="5BEAC7C6320C4E26" modified="2022-10-12" version="1">MEYPQNLLTQGIKFSIIGDNVAQIVLLPGEYIITQNNYIQYYSDNIEIKTKRSYFIVANQVTIVNNSKENVAYVGISAQTGKVMVLDAAIFNNYLIKESSILAANDFIEVGPIPQFQQNYQKIVFSRDLMKQQLIFLKTNGTVIDKDLGDGESITVQKHSILAMQDFVRYELNLKDNTKVKLIGPGRILFEINNQVMPNNLMLSRRRQLIHFFLLMSMLFFLSFVDIMLDIGAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TQI2</accession>
    <name>A0A8S1TQI2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0260254</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8154985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TQI2"/>
    <dbReference type="OrthoDB" id="288274at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8154985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="85" mass="10317" checksum="C0740030A46C9C3E" modified="2022-10-12" version="1">MSIQIANLPEFSYKSIPKRSLDLENFFKEFEMKQFLEECNYSQQELYVECRKMRQFQQKRKLKLNKYINSYLEAPSIISPVQLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TRI7</accession>
    <name>A0A8S1TRI7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0260073</name>
        <name evidence="2" type="ORF">PPENT_87.1.T0260089</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8154648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8154680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TRI7"/>
    <dbReference type="OrthoDB" id="292045at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8154648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8154680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="81" mass="10080" checksum="A095C070974E68E8" modified="2022-10-12" version="1">MEQLQRQPAKQLYFTTELFKNKFITEKEKIQLKEFIIAQDDFLTSYFDQYERNDITEQELREKLIKLVRTDYFKDTKFKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TTX6</accession>
    <name>A0A8S1TTX6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0260266</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8155007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TTX6"/>
    <dbReference type="OrthoDB" id="310389at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8155007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="88" mass="10397" checksum="8ED2B2D08DDB110D" modified="2022-10-12" version="1">MFSEGKNDKESKAKGNDNQINQKQQKKQQSQEQQELERQKFNNKFKFQYQTLMQIGMNGINFEFMYNQNINSKISEIIGEVSLEGIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TWQ2</accession>
    <name>A0A8S1TWQ2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0290089</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TWQ2"/>
    <dbReference type="OrthoDB" id="297826at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="3">
        <location>
            <begin position="472"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="1">
        <location>
            <begin position="472"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="143"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8156628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="551" mass="65313" checksum="7E90FAA8B9207C76" modified="2022-10-12" version="1">MDQMNSKDLLNKINVQLKSAVTVYAKIQIVNYILNLTQEFIDMVGQSSSMKMIAQYLKESLKCIQANEELIDKIFMLLLKMNLNDVILKESEMGLLFIKIKDKRLLEDQRANVNDQLQQKWLKIYIRYRSILEDLKKNNNLKDKYEERKKKVKRNQSSSSNDSSNKKKKNKKKNVRFAREDILMNYKYFKRDDEPNQRGMTIEEVIAFQKLYQGELKRLNQINSYDIKHRESMMEGQEHKLQLDNIQQMIEQIPFIKPLKLRPTYLETYDTNFKETQKQQMRTESKMSAFYNRDIPEQPGYNEISNTQSGIQPRSIHLDPPKREDLMFMVQIICNRGFTELEDKHKNVNKKQIKGILKKPQEEQSKQKDIMLNQAKQDLQPKMQQLVQMVEQKQNHSQESILTLLHDIIQKLKEIGEEQYIKNFKSFLETKLNDKQSIIIEQERIKSQQEQQRTRIEQLQTINPVQAQRLRAYKTKHCHNFHSSIGCARGDNCNFIHDSRYPGRPAPILQSPNFLNKTLYPQSHMQSIIPPLLQQLNPAFLLGRLKKYDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U2J0</accession>
    <name>A0A8S1U2J0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0320114</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8158714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U2J0"/>
    <dbReference type="OrthoDB" id="304365at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035852655" evidence="2">
        <location>
            <begin position="25"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="53"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8158714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="138" mass="16111" checksum="231E19E68665627E" modified="2022-10-12" version="1">MEILHLALLVVISIVCFKIGQGLGQKEVEKQQSQYIQLQENEQLIEEIDEDFIKQVDQQEQDLKEAIKKYNEINQRDKQLQKQLNDLKNAQTNNVKPKVQQNELLSDKDFDNLLDEGFVVDQEINETQLDQDLIEASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U4R2</accession>
    <name>A0A8S1U4R2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0350016</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U4R2"/>
    <dbReference type="OrthoDB" id="303944at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035828503" evidence="2">
        <location>
            <begin position="16"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="256"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8160501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="357" mass="41134" checksum="1312B154ABFDC2AC" modified="2022-10-12" version="1">MKLIVLIVLVGVALARDEYFFAQLRDSEFGKTIIQTLQVQLLQEHPADTVVHLLKQMKDDLLNEQRAEDEDVQGSLQSCQTASEAAAAVITVAKERKATAEDRLPLLQQEQNDKKQQLFDKQGEEQRNLDRISVLQEARNVQRGEYEQRRDELVGLISALQEAKQILSQGITSLKKSSFAELKSHHQNFLKYYPHKKGFHTMVNMLLEVLQDEGTQESAAQKVVKIIDTLVDSIFQVQKEEMKADDGRELDFQTQKERLLLANRRLEGSIADLNARYEVLGQKILEVRNDIATQDAVIFNKQTEKGDWDQTCNDTEKAHRQQTDSRNAQLEIIVECIDIFETRFDMETKSYIQRIRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U5Y7</accession>
    <name>A0A8S1U5Y7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0140221</name>
        <name evidence="2" type="ORF">PPENT_87.1.T0340129</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8160054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U5Y7"/>
    <dbReference type="OrthoDB" id="304988at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8145395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8160054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="248" mass="29735" checksum="CB5346DB221FA0CD" modified="2022-10-12" version="1">MPIVQGKAKLWHPTQEEQDAYDDKMIANIELKSLDFDDENFSPVFNRSKKEFFLQASERYKKDLSKLARPFQSYTCEEFVNKYIFIKPNHTYWREWTVTKWMSGFGLGYLVLRELPLRNFYARVFVMWIFLAKLSDHFTSILPYHGKIVISTQSDRFTNKDINQYHNVCSALNFLEMPTFQNRISESLAWRAKQPAHLLYNDTNWCLHILKKWHGRPTHIAHWDGTFNQPLERLADPYHKDAHFIHWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U938</accession>
    <name>A0A8S1U938_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0340152</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8160099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U938"/>
    <dbReference type="OrthoDB" id="312660at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14003">
        <property type="entry name" value="STKc_AMPK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000592">
        <property type="entry name" value="CAMK family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF82">
        <property type="entry name" value="KP78A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="13"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="11">
        <location>
            <begin position="278"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8160099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="550" mass="64702" checksum="E3A0909F2DC67E4F" modified="2022-10-12" version="1">MSNNELTKQVQNYVLDRTLGKGTFGKVKLGYHTICDEYVAVKILEKRKIENDADFIRVQREIAILHKVEHQNVIKLYEIIESETNLYLVMEYAKGGELFDYIVKKNQLSELAATKYFIQLIDAVEYLHQQKIVHRDLKPENLLLDEQRNLKVADFGLSNIYKDNDQVKTACGSPCYAAPEMLYGKIYGGQKSDIWSCGIILYAMLCGYLPFEHDNTKKLYEMIKYEDYEKPKNISSIAQDLLKQLLTKDPSLRIGFNEIKQHPFYKKMIIQPQQGLLNKDQVVFKKLSELGYDVSNLIEQIKKKKHNSQTAAYWLIMKKISSNQQPIGKQTTQQSQQTQQQIKQNQIQQQIKQLQSFTGGQNSKEKIQQQRQLRYSVPHNKYRIDYSQFQDVDTGIQNKLNNIQKLRKQTTGNIIDYVRQKTYSVQEKKYCHTPEQVDLVIKQPSKKIQLLMQRKKNPTEQIEKLEQTDNGLKEKIKDQAKKSRHKTQPLKKINEKMERYSNNYPQQLIKQNSPQDSFISKYYNRFIRVRHSSNHDNTLKQIKQLTHNFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UCV1</accession>
    <name>A0A8S1UCV1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0370032</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8161803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UCV1"/>
    <dbReference type="OrthoDB" id="309516at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8161803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="171" mass="20576" checksum="826E77AD0B00107F" modified="2022-10-12" version="1">MIEKEEEFVCSINHKLPIYMIVCKKKVEKNKRLLCNQCMDNLESNLNNVMSFRKVALSIEENQKIKVKQVEYNIIKNIKQIDELQKTLHQLKQHITQQLNQLIRNTDEWIKFLQQIGQQYVNYSFFEELDNLINKVSIQQFYIQSFNYLNQLNQSFMVSKDNQQIELIQVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UGT1</accession>
    <name>A0A8S1UGT1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0400276</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8164208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UGT1"/>
    <dbReference type="OrthoDB" id="301761at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="21"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="227"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="295"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8164208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="700" mass="83280" checksum="056EAC0111D17272" modified="2022-10-12" version="1">MNKIPINSPIKDDSIKYLQIIREKEESIQALQTINDQLRQDIIQLQSQLAESFNNKNFKYQKLEESNQQLKVMLTDTQNLVLQYQSDIDIYHQIVEQIIKSTQLYRFNQKTTIPQILNFLNQEVYNQDIVNLWNELKQILKQAINQINIKMSSPQLKEIYTKFYKNYENQYHPLSTMSIFEEIILMLNEKVSQQIETQVMNYSDQFTHQINKINLFETSIVLINFEEDQLKKKLKSQQKQIIQLKEELESQLKINKIFEEQIQTLTMKSSERKPFIEILSIKDVSSSSEDDNVEILKLNNELEQKDTLIQKLQQTIQNQQLMLDRVNIENQYWDLIINCDEDSLFTIINQGWTLNFKQDYGLSNYQVFYSFLGFDSSYLQFILENLFVNDFYFQKLSDQTYLQLANNQLAYLKSIFCNEYNTLIQTKVLHPLVNNFYETSELVEGQQMLNEFWIKFSLNNGILFLCLKNYNEQNLTLLQLAINIQNQIEQTSVLIVIFEDEYTESDVVMKIIQSNEFIGVDDEIDDQQQNIRISMTSGDNFDFNNLNKRISKQKIDYVQQLNQFMKHNLDVYFKKSEQSKLEFNQSLLSENVYEMKLVQSQESDQIKIQTYSQVQVIKNDEIMIHKISDSQYDIFFFEKLQEVRLKSYQINNQGLIIKLKCTFDNDDMITKQYILKSTYIKEDQGEISHTIFDNKLILQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UKA4</accession>
    <name>A0A8S1UKA4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cation-transporting ATPase</fullName>
            <ecNumber evidence="12">7.2.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPENT_87.1.T0410111</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.-" evidence="12"/>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UKA4"/>
    <dbReference type="OrthoDB" id="425043at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140358">
        <property type="term" value="F:P-type transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000023">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006544">
        <property type="entry name" value="P-type_TPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047819">
        <property type="entry name" value="P5A-ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01657">
        <property type="entry name" value="P-ATPase-V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630:SF8">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12409">
        <property type="entry name" value="P5-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="916"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1000"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1039"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="domain" description="P5B-type ATPase N-terminal" evidence="14">
        <location>
            <begin position="37"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="13">
        <location>
            <begin position="241"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF12409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8164593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1078" mass="123705" checksum="64CB7EE6D8A66D70" modified="2022-10-12" version="1">MIELQETNPKEEIDFGQSTLEYQGEYDDQYKLTAIYFRINKCKQIIYYVLLILTLGLLYLFQRWFINLRLFLLFEKCEFGTMTHVLVIKTKNIIPIADDQLCEVTIVKDHPFSKNARVFNYELSDYYIETYTKALVQMRNSFSQYTQERICDMKGLTELTTVYGENIMEIPIKPIPLLLLDEILTPFNIFQFSALALWAYDDYLNYSLFILAITIIQIGIELRDVRQNLLKIQRMIRFNVNVKVIRNNNQITIQSKELIPGDLLIIEGHTKISCDCILIEGNCVMNEAVLTGESVPINKSSLEKNEQLFQQKGNENKMLFCGTTCLRSYSQNGEQAKAIVYQTGFQTLKGSLARSIMFNRTQTFSFYRDSLKYLFVLATLGLIQANITILISGAQGVALGESIINALEIVTIIVPATLPTALGAGISLALKRLEDKSIQCVKPDKINVASKVNLIAFDKTGTLTELGLDVLGCREIKDIGFNKLQMIKDVSEQFNECMGICHSLSIINNEIMGDPIDLCMFQETGWELVEESKIRKGVKEVSVLKRFDFQAELQRMSVITNQYILYCKGSPEQIKSICVNVPTNYNNMLQRYSSQGFRIISCCYRDVAHLNMQQLHKREIYEQNMIFLGFLIFENKLKAETQSTILSLKQSNIKSIMVTGDNPYTAINIGLQCNILDQNSRIFLGFLNNGELYWNEIMQQKTDKADENRSCYSLDSNQVQNLETNEIMKLSCHFQLAITGQVFEHLQYQYAALLDNQQLILNLLQKTYIYSRMKPNNKGDLMLLLRQDILNFIAFCGDGTNDTCALRQADVGLALSLEDASLASPFTSTIFNISNIINLIKEGRACLVTCVECFKFMTLYSCIQSVAVLQCYFYDTDFTQVQYLYQDLWLIIPLAFTMDLTKSYHKLANYRPISNLISLPVLSSVCVIVFMSFLAQIIMNQILKHQEFYQQIKIELVNNDYYMQPNYTNSILLITSSTEILAVAIAYTQGPPFRQAIYKNIYYMIVICLGIIGQIILIFYPNLIEFLNLEVEFPYDFKIYILIVNIIVGIIIIFYEKFVTAKMNQKYAEVEINLGNQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UM80</accession>
    <name>A0A8S1UM80_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Rad50/SbcC-type AAA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0410244</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UM80"/>
    <dbReference type="OrthoDB" id="18797at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000794">
        <property type="term" value="C:condensed nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030870">
        <property type="term" value="C:Mre11 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003691">
        <property type="term" value="F:double-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051880">
        <property type="term" value="F:G-quadruplex DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043047">
        <property type="term" value="F:single-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070192">
        <property type="term" value="P:chromosome organization involved in meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000722">
        <property type="term" value="P:telomere maintenance via recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007004">
        <property type="term" value="P:telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001805">
        <property type="entry name" value="Similar to DNA repair protein rad50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18867:SF12">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18867">
        <property type="entry name" value="RAD50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Rad50/SbcC-type AAA" evidence="10">
        <location>
            <begin position="7"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="203"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="319"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="453"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="568"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="670"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="748"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="841"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="914"/>
            <end position="1027"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8164849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1237" mass="146335" checksum="158F56B231223AB1" modified="2022-10-12" version="1">MSSLLHLKLDGIRSYCHVGQVKEDNRKAAQQSQSIYFKQPLTLIWGHNGSGKTTIIEALRTITTGMQPPNSEKGRSFLTDSHLLGQSKTDACIELGFKSINNREIVARRHFSIVYDSPNSCKFSSLRSTLQTRSLETDKLETLHSSCANIEKQIPCFLGVSVPILNNVLLCHQEEQLWMFSDNSTLKGIFDELFETTDLVNIETRLREQLKDAESKAKILMEKVEFSKRDMDDGQRQRTNLQNQLEEKIQEIKIIQDINTQMNQIQISNINFNIIQQRMSECADLIACRNSIQENKLQLQLDHVDLNQLSQQDFVISSLKQLEIELRNTQANLKSTEINLENKKLELQELQIDTTIETLKQEIKLIRDSIKSIFMFSNNVSDENLLKQVKEKINQNKYEQQQEQQIQQDLQQTFQIKISNLNDKKQKCLIEVRINENQRNEQLRLKDQISKDIKNKEVLLQQLCFQKKQAEQQEIQIKEKQKLKLEFEQINQDLIECYDITQKNQQINSYQDIIKKIQELEEIISTKSSQIDLLIRENGWRAMIKLNGSKLERDISISRCEESIKQKIQSIKTQMAKDQAQIKILNEQKEELKNLLSINKAIKYDPYELDNLKKQLREIKENKIKYIFNQTEFIDQYITSSKQSGKCLLCLKPTDQSCFELLKHRVQQNLPKTKSKINEFELQINSLKAEKEKIQQGIQNNQHLEKIRQIDSQIAEIQASQKDSVNNDSILQLEKNLQDITLLRQLFGNEEENQKEQLLIQKMQMEREYPILKGSLKLSNNPYNQSQLQKAKQEIQNKLKQYESLPENYENPKQLQDSILRIQTELNQLKLQNQKIVIQDFDDKQYEIQQIESQLNQLELEQKQQYDQYMELILNKKQLLNNLESIQPQFEQKILKKQELMNKSKNNYKYADYIQKLQLEIQTETDKMQQLELDIQQKQQKLKQYQELQDYIQKLQKWQDYDQAEEKLNEQIEILQQDIQNLQQEYQQQQINISKKDKFRLEYDLRRSKLEQNFQSALELKKDINRRYDGSEQKRLELLCEYVATQQFISDLKKYLQILEATMLECHGQKMKEINKYLLDTWQKIYNGQDIKFIEVKFDEIPNQKKISKKYNYRLVMRTMNNTEIDMKGRCSMGQKMLASIVFRMALAECFGSNCCFLALDEPTSNLDRKHIKTLAEQLNSLIELMKQHEQQIQLIIITHDMDLVKLLKRHSESYYMISKKDNGFSGIEERKIEELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UNH0</accession>
    <name>A0A8S1UNH0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Arp2/3 complex 34 kDa subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0420128</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the Arp2/3 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARPC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UNH0"/>
    <dbReference type="OrthoDB" id="312975at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007188">
        <property type="entry name" value="ARPC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12058:SF0">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2_3 COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12058">
        <property type="entry name" value="ARP2/3 COMPLEX 34 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04045">
        <property type="entry name" value="P34-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU364015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8165272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="239" mass="28101" checksum="6B3A64EB4F9A9ED1" modified="2022-10-12" version="1">MMLIDPQNKLLYTQIMDLIMKKDPRIVVAKDYNYSCPKLQYKENGRLFLSFTCFNFNEIFSIAGNYMIDKYYKDYTKEDPDVGFHLTFSFNVQSAKEEPKIQKNATEAEKAEIQEIKLQIRAENQKLFEKVTKDFSQIRRNFYASAFEQAFDQINKGQTASKFKYQSRENEVVYAIPDQDALNIFYEISFSDNVDKTLTNLIIDAMTIIFIYIIQPIKLINISKLYSIKKIIILTQIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UR23</accession>
    <name>A0A8S1UR23_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Jacalin-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0460219</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8167081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UR23"/>
    <dbReference type="OrthoDB" id="282290at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001229">
        <property type="entry name" value="Jacalin-like_lectin_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01419">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Jacalin-type lectin" evidence="1">
        <location>
            <begin position="26"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8167081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="171" mass="19465" checksum="93D3279FE72566AA" modified="2022-10-12" version="1">MHAVPEHVTIGLPREGLQTFDDHTELAKHPAPYRIKEIEVQYTDEHIIGTVFKYADAKGHTIDGHDIEKFKPFGHLFGKVHKKHFKIDDDDEILEISGHAGARINELKFKTYKGKEESFGVKNGVHFIYSFPGHTFGAVSGGYEKHLDFIRIHVNELPPAKHHLKIIIVKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1URB5</accession>
    <name>A0A8S1URB5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ABC1 atypical kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0460270</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8167181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1URB5"/>
    <dbReference type="OrthoDB" id="427480at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13969">
        <property type="entry name" value="ADCK1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045307">
        <property type="entry name" value="ADCK1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051130">
        <property type="entry name" value="Mito_struct-func_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173:SF19">
        <property type="entry name" value="AARF DOMAIN-CONTAINING PROTEIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173">
        <property type="entry name" value="ABC1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="2">
        <location>
            <begin position="121"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8167181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="502" mass="58529" checksum="60419A3AC1FA0DC8" modified="2022-10-12" version="1">MIKSFGYLVCGAVMIPSITLAGSTVYLYPEFRTNPAQFWLAFQRVYRVVKAGTNMALAYLTQPDQPMDDKHYYGALQLRQAFQQNKGLYIKFGQILGSLDIIIPEPYRATFEVMCTQNIETPFDDIERQIQLSTGKKIEELFQTFSKKPISSASIAQVHQAFLKDGREVAVKIQHPWLKEQIDGDVKLISLFTDVAEFIFKGFKYKWLAEELQVNLPKELDFHGEINNAKRIKEILKPFPDIYIPKVYEEYCNDRIIVMEFIHGTPLSDILREKEKHDFNYPKIAHTISTAFAHQIFKHGFVHSDPHKGNIMVRKLNGKQQVVLLDHGLYTTLTEKTRLSYSLLWQGILEMNLDYIKQATNDLGAAQNYKLFASMVTSKRFSDLMNTSMNMQDRLKRPKDQDEIAILIQKAAKKHKQITIILNQIDRKLLMLFKINDFLKNIEFRLGNPIDSCQITYKYVSMVNYEDKIKKQNIFRRMMYKFRLLLHKAKFEILSIVMSIIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UWR1</accession>
    <name>A0A8S1UWR1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Homogentisate 1,2-dioxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0480132</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000048">
        <property type="protein sequence ID" value="CAD8167989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UWR1"/>
    <dbReference type="OrthoDB" id="1689029at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004411">
        <property type="term" value="F:homogentisate 1,2-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006572">
        <property type="term" value="P:L-tyrosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07000">
        <property type="entry name" value="cupin_HGO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000034">
        <property type="entry name" value="Homogentisate 1,2-dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046451">
        <property type="entry name" value="HgmA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046452">
        <property type="entry name" value="HgmA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005708">
        <property type="entry name" value="Homogentis_dOase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01015">
        <property type="entry name" value="hmgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11056">
        <property type="entry name" value="HOMOGENTISATE 1,2-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11056:SF0">
        <property type="entry name" value="HOMOGENTISATE 1,2-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04209">
        <property type="entry name" value="HgmA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20510">
        <property type="entry name" value="HgmA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="5 8" id="KW-0408">Iron</keyword>
    <keyword evidence="1 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0585">Phenylalanine catabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0828">Tyrosine catabolism</keyword>
    <feature type="domain" description="Homogentisate 1,2-dioxygenase N-terminal" evidence="10">
        <location>
            <begin position="12"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Homogentisate 1,2-dioxygenase C-terminal" evidence="9">
        <location>
            <begin position="285"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="296"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>homogentisate</name>
            <dbReference type="ChEBI" id="CHEBI:16169"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="375"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="375"/>
        </location>
        <ligand>
            <name>homogentisate</name>
            <dbReference type="ChEBI" id="CHEBI:16169"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR605708-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR605708-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF20510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8167989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="443" mass="50421" checksum="DD6FF415E89D7BE2" modified="2022-10-12" version="1">MDTHRNYENLDYAIGFGNHVETEALKGAVPKGQNSPQKCAYNLIAEQFSGTAFTLPRASNQKTWLYKIRPSAAHTPFVDAPEFGKYVKNDFLNDHGLTITPNQLRWKRLPLPQKPTTFAEGLVTVCGAGDPSIKQGIAIYLYAANKSMTNSSLFNSDGDFLIVPWEGEMLITTEMGKMTVKPREICVIPRGIKFSVEMTKPIRGYICEVFKGHFRIPDLGPIGANGLANPRDFLVPTAYYENTEEEFQIINKYLGKFYKCTKKGSPYDVVGWHGNYYPYKYNLDLFNAMGTVTFDHPDPSIFTVITCQTDEPGVAVCDFAIFPPRWMVAEHTFRPPYYHRNVMSEYMGNISGQYDAKEDGFQPGYGSLHSILQGHGPDHESFTKWSNIDLEPIKYPYENLAFMFESTYMFKTCSFVMDDQVNLDHDYYHCWDKLAHNKLGDKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UX43</accession>
    <name>A0A8S1UX43_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein kinase domain containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0490095</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UX43"/>
    <dbReference type="OrthoDB" id="191686at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000585">
        <property type="entry name" value="Calcium-dependent protein kinase-a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001132">
        <property type="entry name" value="Si:ch211-285c6.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000591">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001330">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="32"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="349"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="386"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="422"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="458"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8168432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="489" mass="56302" checksum="E105F0152C44A66F" modified="2022-10-12" version="1">MGCAAAKPEKQERSLSKQNSPCSHPKEATPSISLMQETDKIESDYIFHSLDPLVTRRNYIKKAVSKSNNASRIVKVYNCLNLTTTEKQELERIVNHLLQIKHLNLVSQQQYYYNDNKLFLVSDFCDGGNLLSRLDHFSQLQQHNMFDVFCQIMAGVQFLHNKGIAHGNIQLESIVFTDKSMQNVKLIDYGISSSLKSTCLQWRPNGGIQEISFKSPEALKQSNLTSFKSDIWSCGCLLYFFLTSHMPFQSRDVQALKTAIQRGIVNFEGSEWTNINPDMKQLVSKMLSSNPQLRPTASEVINHPIFLNRAKIITKPNKQLSKQMKDFKQQSQMQSAMLNYIAENMLQEQDKKKLMEEFQKFDLNKDGQLTKQELLTVYTTMYSSEQANQEVEAIFSKIDQNGSGRIDYQEFVLATIDQKKYFNREKLLMLFQQIDRDHSGQLSKLEVKKLLRDMQVPKEKLDQLSKQLDQDGDGQIAQEEFLQIMLSIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UYE3</accession>
    <name>A0A8S1UYE3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0500227</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8169233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UYE3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="3"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8169233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="282" mass="33490" checksum="A0FAB65431860EED" modified="2022-10-12" version="1">MKYQSLSNDIRRLKRIEQNKEDQLNKLISNIGQEIQQNIDDPYKYLIKRNYSIDDRTIIKRDDTQQRRPQGSMSIAEIWTKKMKKQCDKILEEMKKKQNKQILDFSKQQSIDNRQIEESNQSPQNASLMIEQLGLKALMGKTKYKNKIKQIVQSPQQKLQSQSQILTQSPQPIRNRFASNFHFEPIQYSPIQQNHHNSQSTSSSTKNLINSNFRKYSVNNEVKREVNKCIYDCDSLIHNYKDVDKKLKQPQKSRLDIFVEKQRKEALEELIFIERNKPKNIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UYE6</accession>
    <name>A0A8S1UYE6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0510101</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000051">
        <property type="protein sequence ID" value="CAD8169524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UYE6"/>
    <dbReference type="OrthoDB" id="10063692at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="159"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8169524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="298" mass="35702" checksum="0E04EE644033BC12" modified="2022-10-12" version="1">MNQLYKLTPQELATRKDIVVLSDKGLRFREEIKSQGQFDLDDFHLPQINQSKDKPYTRKQVLQGEKPVELLVADLKKELNISRPYMEKALEKMTIKITQEKPSAAINNKLQKLYQELDKHTRLLEVRKVKFQKKHIDQQIQDQTSILEESEFQDKVASLTALEARLSRMQQNYQNEIRIRDDTLKKVKQNLKVMRIQKEAEIEQIPVPVQMDKLQQSQYLDRKQVRLKDYQKYKFEKVWSRQPPPSYTSKQALIEEERKKQLQLIKEQQEYEQELLRRNGHQNINNIANRTDHEQHEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UZD0</accession>
    <name>A0A8S1UZD0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Prenyltransferase alpha-alpha toroid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0510120</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000051">
        <property type="protein sequence ID" value="CAD8169543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UZD0"/>
    <dbReference type="OrthoDB" id="10261146at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005965">
        <property type="term" value="C:protein farnesyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004660">
        <property type="term" value="F:protein farnesyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045089">
        <property type="entry name" value="PGGT1B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="Prenyltrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774:SF6">
        <property type="entry name" value="PROTEIN FARNESYLTRANSFERASE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Prenyltransferase alpha-alpha toroid" evidence="3">
        <location>
            <begin position="54"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8169543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="367" mass="42142" checksum="DD89738AD61035E6" modified="2022-10-12" version="1">MKQLNRTLNEQNLVHSAISQIRKEYQYVESEEVPQDLLENKYYKEYEPLNKLELLTDQHIKYCTKKLLNIGYSIFYLDVGQPWCIYWPLNALSILQDDVSKYEHQIIQYLEQCKIGSFGGGPYQFEHLAPTYSSLLSLFILATPTSLGLIDRQGLEKFFWSVQDPNIKGSYLMHVNGEADMRAVYIVVIMVIILKLDPKLLDGCAEYIASCQTYEGGIGGVRYSESHGGYTFCGYAALVCMKKAHYIDQEKLLNWLVNRQIENEGGFNGRTNKVVDACYSFWQGAIFKLLMLSGYVNEQLMDVQELKKYIHICQNISGGIFDKPSKSPDAYHTCYGLSGYSLTDSNFQNPIYNVPNKCIEFVQNFFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V0T6</accession>
    <name>A0A8S1V0T6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0530007</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V0T6"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000586">
        <property type="entry name" value="Rab family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="177"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="182"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="193"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8170334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="204" mass="23330" checksum="1E786179705D7190" modified="2022-10-12" version="1">MEEQLYKIVIVGNSAVGKSSLLIRFCDDQFRDMYLSTIGVDFRFKSLRVNGQGVKLQIWDTAGQERFRNITNSYYKGAQGIVVVYDITNLKSFEDIRKYWMNELNHYADENVLLMLLGNKSDLATDESRQVTTAMAEELAKEFNLKFFEVSAKTSDQVEAAFQAFTQQIQETGIAGKVRKPQPQQFLQTPVPQEEKPKKQDGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V205</accession>
    <name>A0A8S1V205_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0550004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V205"/>
    <dbReference type="OrthoDB" id="305067at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="79"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="177"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="278"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="783"/>
            <end position="824"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8171398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="918" mass="109760" checksum="539EB82778C6040B" modified="2022-10-12" version="1">MRAQSPTSKFIPTSTITTTRLVSSPHSNIRDQRADSSVNSRVTKITTYQTRPKTTITTTTTYRPVIETRTVRLCTDKRCQGHEQHIEQLTQENQQLHQRIFELQQEIEQTSLCQRQHTNTYSCEIDNIRKLYEESQINHKSEIEYLTQEITQYKTQFQHSDDQVKYLTQQLRKQSSNENLENQITLLTTEMERLNMIILEKNNFVEQLTQKINELDSELINKENIIENLESNSSKLRKQYQEACNQVVLIRSELDRQLETIQQQERNIFLLNQDKQAIEQYTFILEQKEQQLQQLRSNIQLLEMRLQEQQTTIQAQYKEIDNLMLRLDSANQQIDYYQTESDELQKYRNILQNELEMSKQENVKLQDQITKLKQQQTDLTKTIEKLKLDLNQQLSSISQDFNFELQSKNDELEQNKSQYQLELTVITKKFQEENYQLRINNEDLQNEINQLNNVNQILQNTVDEKINDISNLKNQYTQLYSEYNTYKMKLQEENHKLKLEIEDALITSNQLQQKKNSYESTIMELNLVINQLQQQITQQQNELNSLKYKSGEEYSTLRIQIEQLDQNNIQLQTIRSRLQKDYEEKLNELKDLQDKFNQYKQTTQQTIKDLEKKIYEYEEKLSLLSSEIQRMLFQINQKNDIIQEQTTVLDEYQREIKLLKQQLEESQLQAEEQQKKLELQMNEALTQQREKLFRFESENLSFKSFIEEQNHVIETLSRQIDGMTGDLKYQQQLEEELSKKNSFLTKQLTELQSENAENQFKVEHLLVENKSLKQELEIRIDTESELKFKLDKLIEELAKKQQQINEFEQRIKEYESNQSNEVVQFEEKITYYQNEVETWKKKFIVLNKDYHKTQEDLMMVQAEFDAFKQRGNPTVVRESTSFEVRKSSLYKENISNTKSSQVSQSSQIIVKPLKEVNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V4F7</accession>
    <name>A0A8S1V4F7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Myb-like DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0530218</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V4F7"/>
    <dbReference type="OrthoDB" id="293672at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF253">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_38, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="130"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="2">
        <location>
            <begin position="150"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8170732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="322" mass="38490" checksum="19482667CB0C68FE" modified="2022-10-12" version="1">MNNIRQSNELQFIKRKSPYILQEAPQKLVKISIMSQTQFKDFLESLSLNFTQLCHNLQQMITLYHVQIFHENDDDLLLTNSDTDAFTKFQQDWLIKTICKVGVNHQFWPEIAQVLNKSLQEVKAFWINLQDNWTNEQDNKLTQLRNQQLNWLEIALRLDKNKTNCKHRWNNVLDPNLCKTPFTIQDDILLLQKAIQLNFDWKKIQKIIQPKRSQADLISRFIKLQSQYNNIDNLIQRVQFTIAQLPKYDKNSMRILPQQSKKIQKTVVEYETLSILDLNLFDAMSLQFCLVKHDQIFFVTLEYMQQLVNLKLNQRKLLQFMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-02-22" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V9T5</accession>
    <name>A0A8S1V9T5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0600166</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000060">
        <property type="protein sequence ID" value="CAD8173980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8173980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="289" mass="34604" checksum="6CE86916B3ACC746" modified="2022-10-12" version="1">MSLIANDGSIDCNIQTLNQLIMEFYLAPFNYKHFGSIQVRMFKMFEKKIASDQESDIIEIFKICLRDYASELLQEFSEENFKQVENLWEIHYRLQATSLKTERLYEIYYGLFKIKTPCPYDFFQIHFDIAKKKRSNLQDSRKVHQQYIKERMKQKVTLVKILQDQCLSQKPQQQNSECNQLKSIQKNTQSIQKQKDKLPTQSTINVRNEFKEFEDRLSKFENSSNKLEPTFSASWTQKYRNLFLKRKXVLYEFRTSIYFIQFLKSKICKKKDAIVGLLIILKQQIQNSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VA68</accession>
    <name>A0A8S1VA68_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Myb-like DNA-binding domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0590142</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000059">
        <property type="protein sequence ID" value="CAD8173471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VA68"/>
    <dbReference type="OrthoDB" id="306004at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF274">
        <property type="entry name" value="MYB-LIKE DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="85"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="144"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="144"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="205"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="210"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8173471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="521" mass="61738" checksum="171625E5539CE051" modified="2022-10-12" version="1">MQKSSRSLSSKKNEKLISKRNKSLQETESYGKFLEFYNSNQNQPFKQFLYMQEFFNTNLNSFPSDEEIQAIQQNLFSVYQVISNRKKNWTTDEKKVLIWVVGKLSQSQDLDIRDLPNSFWDDVSELICRRDPQSCKQKWLQLKKTDLQQKPFTQQEDQLLLDLIHKYKDSEQGKKWCQISEELNSQILNYRTSKQCRERWLNHLNPKISKDPWNDDEDIFLLDLILQQGRKWAEISKLFDGRRNENTVKNRFNSLIKREKDLIVDLQNGQTTNLDTIFGNPTGQTLNKQQIMAIEVLKNKIQWRKGQIKSGEQINDFRRTSIHQTSDKQEEFQLKRSSVVKYQVGHLGQDEIQNDELVPCLVNLEKNIIYFCSKEQILKLLDNQINQMNYNLELAKKDIKSFDSGLINFKTQLSIISEINDSKFSSFLCGQNNEDNFSQISRVFQNNFIQKQMIDLEQYFNLDPPQHQQINQLFNPIQQIHYKAYYQVRRAYKSEVLQQNVKIIDQKIPRSLPNVISIISQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VG58</accession>
    <name>A0A8S1VG58_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0630004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VG58"/>
    <dbReference type="OrthoDB" id="300641at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="327"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="351"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8175335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="718" mass="84401" checksum="6422BFD9A01EC7BC" modified="2022-10-12" version="1">MTLVGDLFYYIDGQILQMEFQYCHNSDIQYFISMEILYIKICHNSCLSCFGPSETYCITCPDEESNKTIAVDVEPLTQRMKILNVLKAYNTFQILLFWKVKILIHLPVVQVSFISSLGKQYIVMIVQAIRSQILLIKGNYYVLNQQKTWYSNPICTEDYEQLIQSSTSAYQKQARKPIDYEYFLINFNSNMDKIEIEICSGCLGETRANSDYIVRLNFEKEIKVQCETCYQIINGECINIYSNCDSCDSDQCISCSQGYTLYQNYCYNVSLYVFRIVYIMDRNQVVQNVKQVVTQILQIMNDCNEGLIVLYFNQEYQQMEISFKCSVLNVLITKNFLLTLIIFIANQKLCLIAFINIINSIIVLLIRMIYIHNKVNHIRMSDLIITCSWDYNFSKPSSYYQTRCALCEKGYLYNFYEYDWGKDKCILESEFTDLEIPKFNSLQIAPEPGALGDQTATVDEKVDNDTIVQFRQKGKKKIRCNTLIRERFIIRMLQKKSQIKYIKNNGWFNEYCILYNQGIMLNFNQVNVTYLRRSYITEHMDGNGKSGHITVQELAQNNDLAIFILSVPQLMQMSMKYFLDRVKKDMIMKNAFQNAVVPNVLYKMIHMFVQNVPHLQYIPINLINNQCTDCHAKCVLCRELNYDENRQINPYFNSEYSQIAIYSKSCIKGYELNKTLMKFQYIYPYPILNTQILCIHSQNYNLQYIAVQIHLITTWIFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VHT5</accession>
    <name>A0A8S1VHT5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0640170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8176103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VHT5"/>
    <dbReference type="OrthoDB" id="291915at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8176103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="228" mass="26170" checksum="FCE71D8C554330A7" modified="2022-10-12" version="1">MRSTYSPIRSSPCKFDIPITKCTEIQQRLDYQTWSDVKKGIRDKVQSRVQGFENQKFIQKSDLLYNFKNSDQKISSQALALRSSTSYVKNHLENKFPKQESPIKMGLTHAIYSPSQKQSNLVDDIKRSNIYPSAFGSFTDKDNYKTYQSKRDLMGSFKSNSDIIDFSRKSKPITDRVSLTEIIKISNHLSDLQLTEISSLSNGYLSELVGLQQNINKLLKNANSKLYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VMT0</accession>
    <name>A0A8S1VMT0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF4378 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0660062</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000066">
        <property type="protein sequence ID" value="CAD8176909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VMT0"/>
    <dbReference type="OrthoDB" id="304761at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="182"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="237"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="315"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="671"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="15"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="569"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="194"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="212"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="245"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="315"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="671"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8176909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1012" mass="118477" checksum="3291700AF06AE04B" modified="2022-10-12" version="1">MNEFNDFKQLRQNYSNALDKNIQKLQGRLKALNNNIINSTSAQKITMTKNPTEVKSLRSSCLFTDNNLASPTDRFENSQVFANFNRYNSLDVPQASQGVQQSKQSPDVFENIFLKQSYIPYEQKQIKQANKTKQKNTQSNTLKITKKAEKNHYLSNQMNSQSLQNIKKKDDISQDKTLKMNIEKKKNLIPPAAQQKTKNTSRSPQKQRVKQNKQQTPQQNLQKPLFNEILKKFVSSKTSLSRTISKSKSKSKSKSKSKSPEKESTSFKKKHKSREQAIHHYNEQITSQFSPTQEKFQMRNNIDYSPSLLRQVRTLSKKKGKSKQKQQQQQQQQIITNPSPNKVSEIHTLRLKHLLRDKSNKQDKKIRTQIRTTESPIQIKDYKIDVPQIKSLEDQPKDESKPSSVRFNTLSIQRSDEKDNSVLMNIFHELKNLSEDQIQQFQQILKQIRVERKDEELQTSISDLNQIVGNQKSNNQSNQKNNIIETHLKMALEEFPKKSHASSPMSNSSFMFKKEFLTNSQIEEICQQREKSIKLRVNSQMQAFQTLLQQQKISPRSFHHNKQLVQSWFSKQADQLQKFKSELQKVQKQSNTLLKKTEQDVIIVKDLKKDQMLVLSQLQQISFDQSEIKDESFLDQSDLQFSIPNSPKIVTPNTKFLNQNYDKNEMKIIQQQSISSSQQKQNQFSKPFQQDTKKKDFEISTFNEELFFGNQMTRPLQVSPNNESKQVITDYREPQVQIYGLNQALQVEEETEKIIEQLIDEASNSLNKIELIQQYAYQTNAGILTNLGVINNFINLMCYAILESNQDEFLKKMNTPYGRKPINRLRQLHGQDDFEDHQEIQPLYQIKQIMETQNQYEKIHNQAIFDAFNEALNTLRPFYQDEGIPYPWCIETNLSQRVCYGIEDITIILERARQKVFDWSKTLCGLLSDEIPVISVRQLNYEQLIIMQECQANDSLAYINEERIQSCLQIELQEGERKWSQITQEYSEVLMEIADYLFEELTEEMVVEVFMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VQD0</accession>
    <name>A0A8S1VQD0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carbohydrate-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0710150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000071">
        <property type="protein sequence ID" value="CAD8179247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VQD0"/>
    <dbReference type="OrthoDB" id="61321at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035734844" description="Carbohydrate-binding domain-containing protein" evidence="1">
        <location>
            <begin position="17"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8179247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="164" mass="19067" checksum="6563DDE9E993D373" modified="2022-10-12" version="1">MLQLILLLLKIANEDQLNTNQLIKMNVQQKIFIDAPLTKCNDPLYKHDVDEIFIGTKTEYLEIEISPNEALFYATIGNPNGTCSNLITTYRQCDEITYFAEFVTPTEWYAHIELPISMFTQPIYANFFRVNKMKDNSTHYMSYNPINASPACFHRPDKFIQLIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VTI2</accession>
    <name>A0A8S1VTI2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0720095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000072">
        <property type="protein sequence ID" value="CAD8179543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="299083at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1054"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1158"/>
            <end position="1180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1372"/>
            <end position="1389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8179543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1429" mass="168825" checksum="D62FBF0B1B706C5A" modified="2022-10-12" version="1">MKEVFQKLTNYLIRVYLKNEENQIVFPPYVILLFRVQQAFLSLSYIYGLSNYYYIYMISIYARPQQYFIDSEIPQYIILMLSVIYIFTLLTIIPYNNIIGIFGQLLRSSMLNFFFEISKGSISIITHTLLVLISFSETLIISGTLNVQTKNFQHTRFTNLDLIVVILNYLLMIFYSYGFPQSIIVILAIILNILRIINQLLFSSFKNLITKTILLTIKIITFFFGWFWIASLQIHLSAEIVIIPLIYKMIYTYYQNQLRNIVYNNQKHIPCHIISLLAFLENVKDIQLLYVSKPKKPRNRIIYSSFLIYQNKNYQAFLELNSIQDDQLSLIESLKKKIILQKCINNIQQIICGTNKIDNIAHTIKLLLNSEEQNYSIQNDIVEIVQNKIHCLEQFTQHQSHNSYENYHNIIKKIINFKEKLEKQYKEFPCEKTQGILGFFYGEIMNDYLQANQLFNLVAMQDEKIKKITLNQDIFSNKMIYLILKFDGKLIIKRPSSDAAQFLQLTNQQLKGLELSYLIPKGVQEVHDDLVLNFLKYGKSKYMRQLNQCLLYNHEMECMSSVELLFDINFVEELNFIAFLQPPANQTLNLILNEQHLIKCISQQFIQELGIEQQYKQYQGNHINKLIPNFPKYFFDKQQIENTEIYVEKQIDEQSQTSSHPLVFITTFNLHFGIVNDITKYFIISFDYFKRSPFYFCQNQSISVPFMSPLIKKQNHCSFAIINDESTIHVPYQEDEVMKNHYIFQLNQQDFNFLDNTPTIQDQKKQQTKLLSTLDYTNIKSTTKQNELLLAEQKFFSKPKIKSSISNGDQVIFMQEVQSSQVSSLQGVRNSKYFRQFEMIAKIKDLNSFSKLHRIFLASIILCVVFQFIIQTIQIIELNFNLKDLVSDIDILQIKNFVFQPLETFLLTRWTIFNYKLLNDAGEISNLEYQNLIQFPLSNLNLGYDSLDKNLKQVFNRPALQNFLEKTYIEIYEYVTTNQGEMYNLNLRNSISILKNFQYSFKMAYELDGGIISDSPYVYFQYKNYLTIKQQFSELNSIVLDQTLKRSLQIQSKLSIITFICLGALIIQYSFSVIYFILLQKIVLKHYSLTQYLQIQYIEFEITYLKKLIEGVNQNQNLLFRYQFRLDNKESLLDSTNLKIYQKKRNIKQLSILNYHDIYYYLFFFILLLLTAGNASLTYYEGWNYLEKYPETAKFYKEVSDVGTDVPTMFAQRDVLYSRKAIIPYFTQNEYDSLLEEIVESLNRTKAFIQDDYNFDKYVVSDSFKGFFTQIQNDSLCDFIPLELKNKSNNICQIVLNQNMKRGLQALLIYIINHITTDMEINQFTSRSQVSYLELEGAFLVSNIIKVVNDKFNIDLMDQTKYFMNLINMHNIIMFINLFFIGILIITYIKNKLIEKLHIAQRLTYIMPSKSIILDSNFERALRSLLQDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VWG8</accession>
    <name>A0A8S1VWG8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0750234</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000075">
        <property type="protein sequence ID" value="CAD8181161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VWG8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8181161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="115" mass="13885" checksum="7A4876EAEC34C617" modified="2022-10-12" version="1">MILKLFVLFVQKDMNQQIKSVCIKAYPDSCLEFEYLNGGYQYIRCYFQYQGRRQSLSENYCIEFQQNFALNRIMSQEEIQLINPFFKDDEQKVASYQCLKGFDRKLHQLHQSQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VWM5</accession>
    <name>A0A8S1VWM5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0750003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000075">
        <property type="protein sequence ID" value="CAD8180733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VWM5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8180733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="248" mass="30196" checksum="7D3674587F2FED75" modified="2022-10-12" version="1">MFWRIKNFINNFKTCLTLNNDIKKKKRNKNYGYFLQFFFLVKIKIQRLVINSIKIDLLSLNATPLNLLFQKFRKKNPFTNKFGVKGLQDNFLLTIVIVQQWQIRNSCIQHQLQRSSQAKTNFLYFLKQQNGFYLKIILKRYTYQKNKVYAPLSKARLKLQSQFKKKQSLLHYKQQHQLKQKLCIQFIDTKQTIVINLENKKRQLLELYDSFIKNQAKFCCRVQWKRIYFKKHKIRIKQQKDNQIIRGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W0P5</accession>
    <name>A0A8S1W0P5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0770094</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000077">
        <property type="protein sequence ID" value="CAD8181832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W0P5"/>
    <dbReference type="OrthoDB" id="9332038at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14210">
        <property type="entry name" value="PKc_DYRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF22">
        <property type="entry name" value="DUAL SPECIFICITY TYROSINE-PHOSPHORYLATION-REGULATED KINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="153"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8181832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="563" mass="65571" checksum="69B04171B085B3BF" modified="2022-10-12" version="1">MKLADRKKFSVHLDMSALKQIIQTNHPLSTKRKIDTTPTSPNTSGLASEKKFKPVQPLHTNINLNNNNNTIQNIILNFKNKTNKEKENAPISTMSKFPLLVDQFIRLFNLTKSEILEVQQLKQVYYYKQSKIQQDGQNGEYLCYAKDHIKYQYEIINLIGQGSFGQVFQVLDHKTQKTFALKIIRNQEKLKKQAQVEANLLMMIKEKDPLNKSNIVRIEEQFNFRGHQCIVFEKLENNLFELLKQQKFRGLDYETLRKFSYQILVALNYLQKLNIVHCDLKPENVMVQDMKSKIIKLVDFGSGCIDGNQIYTYIQSRYYRAPEVIFGLKYGIEIDMWSFACLVSEIHTGQPIFPGDNEIEQFNLIMEVIGAPTTEFALKCPRKKHFFDENGQPKKVIKTYRKPQSVSLQEILKTTDDDFVDFLQRCFTWDAESRLKPQDALNHPWILSINPKENTSKLNNKFFKEIDSNSTFKKSKTQSNTILKEFEKKLSKDNIFLKLQQTRTLIANNTFENKPVLSSRYVNDKPTSSDRIRSNIAHSIHQILSCRQHNASNTQFIRKPSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W1S2</accession>
    <name>A0A8S1W1S2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4460 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0800074</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W1S2"/>
    <dbReference type="OrthoDB" id="299896at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028031">
        <property type="entry name" value="DUF4460"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027986">
        <property type="entry name" value="TCAIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31596">
        <property type="entry name" value="T-CELL ACTIVATION INHIBITOR, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31596:SF1">
        <property type="entry name" value="T-CELL ACTIVATION INHIBITOR, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14687">
        <property type="entry name" value="DUF4460"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4460" evidence="2">
        <location>
            <begin position="14"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="303"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8183091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="415" mass="49018" checksum="20CA51019D7CFEF9" modified="2022-10-12" version="1">MLQKIIVAFSSTANTFKNKSKLTEFYKAIHPDMLQNAPDNVKMENTRSLKILNNYFDAVSQNERTEPQELVFYMAEKQNTKARKFLPFTIVLDAYLGKDQQGAIDKVTTQIREQINQKYQEIRKDFEEKHANPFVTYTNQYEKVKKNFFETTTSLKKRSSTKESEQLALRASIFSAQMQSEYDNFLKNANQQMRQANMPQIKPRLWASIMLDKCDMIPNLFQIFDQLIDPRLIFFDQDLTQEQSDIFVEKMIPKTTNLMNVQALSPFFSRLEKSDPQICLYVTKKYGAKDIPGYFSIPYNFDVQEIQQQFGIYQKQIKQEREEYEQELKKYQQSLYNLADRYYITNIQNEVLLKNTEVVKKLNLSLLILQKQLNEVNTERLNNLRMKQWVLTESVSKTVDSFDHFYIPIPFSGTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W5I6</accession>
    <name>A0A8S1W5I6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0800155</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W5I6"/>
    <dbReference type="OrthoDB" id="6050183at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16463">
        <property type="entry name" value="RING-H2_PHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45943">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MYCBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45943:SF2">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14634">
        <property type="entry name" value="zf-RING_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="97"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="331"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8183249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="574" mass="66950" checksum="C45F9C6F3BDA63B3" modified="2022-10-12" version="1">MQQFQQIQVPECFVCACGFSCLYMKEKDMEFHIDSCPVYSAYSEFMKYIERKDIQNANEDQLRTMKAEAKVYISRLDMMLMIYSQQQQPILQKAPSQTVQCEKCKKQFEANSDFDKVWYLENCSHIICKDCMLKICKDDFLPKKSNVTCVCGEKFKDQEIKQILGNELYEQLTEKLNLSLQNIIECCNCKERFCFQKGNIEEKIQDQNGKLVQGEQLKHYIENRFKCSKCHTEQCKNCMSVPYHTNMTCEEYKINKAAVKCRLCEQPTEIQKNQPEALQTICQQQDCQNRSKKLCTIKLKCGHFCQGLNNTPCLPCLNEKCAKDQNEDDYCNICFTEALKSQPCVQTTCGHIFHEDCLRQKLDAKWNGPRIVFNFMKCPLCNKFLDIQVPHFKNSIEQGQILLKEVQELCLQRLKLEEKEKDKELLDPTHQFFQKPLDYAMHIYCYYLCFKCKKPYFGGLKNCQQAADQDPKVEFKQEDLVCTKCCPLLTLEDKCNKHGVDYIDFKCRHCCSIALWWCHGTTHYCDPCHRNIKTNMTKPCPGPAKCPLGIPHKPNGEEMSLGCSLCRAERLKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W7J8</accession>
    <name>A0A8S1W7J8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0840071</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8184917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="309951at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="116"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="212"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="668"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="826"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="929"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1042"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1143"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1349"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1637"/>
            <end position="1883"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8184917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1974" mass="237008" checksum="FA9F6643511737C4" modified="2022-10-12" version="1">MFSQQTDQKEVITNFPENTDKILESQNELFDNKMRIILMAIEIERLHKILNSKIDYYTQKMQDYIKRELFYKGQIVEYLQRNTELNQQILEKNALISHNIKENSQQEMNMLITQQCLQFEQELKSFQLKNLQLQKENQELRSQFEDAPQLRKKLYDLEQLYSQLLIEKNEEIKQLSQNNQECTLKNSQLDTLNQLLQYKELELLEWQYSSQQTNLIHENQRLKKELKFLNDKLNVLLINNDHLKVKQDDIQNQNYCQTTIPNKIFTQANDIRNQTPRRQQINPTQDQLFNRQNTPSNQLQSSQIPINLQSLSQPQRDNINIQNDNNNNYLFKELIEQILFIQNQKTQSKQSIQNQISLLIKVLNLKQQEISQFIYQKNNYVINQKLSASPQFITNPQTKKMQKYYSLSPNNCYSQPYVILQPRSRSPINQYHKGIKTETTFHSYSNMLRQQITPIVIKPQNIVTYNYSKPQTQIHYHFSPIQKTVVKQSFQNPPYIVRNTQPNVAVQFYQPIKINVNQPNLRSFDKQEQNLNENMNQPEFNVYTTEFQVNSQENINKGESPQKNQINFPKQDIINDKSQQQKSHLDGRLPQNVFISNEGQSLSQKSFEIYQEEQIFNKITLQDPVSIIKQKLNGQVQSQENTKDNFNIIYQQFQDFRSIFKQKLYNKEQEFQKTYSNALEQLQKLENLILVLVNEKTQQISNSRDNKFNRISYEKIGTDQQREYQSYKIEQPNSDYFLIKKQTQINEANDFLQQITKQKKLLDEQRNHFIQLISLQDEIQNKYEPLVSILFQKESELNKELIEIKQDKEMQKLNLTLRLQVQSEEITKFKQQNSYLQQKLNDYKNELKDVQLKLKFEHDQHQITKQIVQSLQQETDKYQDSTSEIQKRNSYLEQQIKQTEQLLFEAQTNSKKQKLSYAKETQTISFENQQTIQQQIQNDQIEIENLKQKLCFEQEFTNQLNDSISTLQQQIQTQEQLLQIQKLNNSNQFDEIQNLTKEKIEISQQLKMFQVKYLSLYEQLDQIQKQKDIDQNNEQIQNIDIIKNLNFQIEDMKQINQFLSNQISQIQSNSEIQISAQNTVLSSKIALLKKQLASEKQLKEQANIRIMELLKQFQGLQNHIQQDETSDLKEQIIKLSNQQQLQQQQKQNLIESFEQNLNQLKQQINELTHQNSNFQEQIKDFETKQLLHLQLQKQFANQTNLINQQQQQIVILQQENNQNQNDLQKLRQNQKLDIQDFEDHQNDQINYLKQQIDELNLYIQNQQRDINYLESQIQFEQREKNIITQNKTNLDRLIKQMQEENIQLQNQIQIQQNNIESLSKNIRQSNTIHNQLSQTDEYYQNQNEIPKLDNQLKQDYQILIQEYQKLKDALYQITEEKNKLEMILNYQNQVQESKFQEIKQSQTIIKIDKNEEKNQLNQNEFKNKQHQIKDLDIQISAALVKLDEFDDLNIRNEQIKYDLQIQIQRLKQQTLYIQQLRTEQIEQDKNKDNIQQKYPSNNFNQKIDELIQIIQILIISIGDHIQLNSKIVMNTQMLYQLLVEINKIIESFQYQNQTLKREGNLTEQQNYSTTQKDYQKQSIELIIENDQLKLKLNQYQRLLIQLQDEYYKQGSALDNITKEYQQIIDQNEYKVIKLIDFEQLNNTINKQEKQIEEYLDQIQYLQNHFDQEQKKQLEEGNARLNNKRQENQKQMDNQIQIIQENIKLKQEISNLQSELQKQNYQEDLREEQKLIQKQSQEEYTQLKQEAQRLYQRLLQMSNSNNILKEEKEQEQQRYQRECKQIQENLEKHKNENIQYKLQIDRLSKQLQEQKSKSESNLQQEFNKLQYTLDASLKQQKQINDEKRVIQAQVQQQQEQLANQLNIINQLKKEISKQEQIISQLSQQNNIQSSFVQEVTFFEDLKNSSPFEFKIKQLQNENSHLHQLLIDTENDYSHKIKLQQLQIQGLNDKLREMVYEQNKKNPKVDEFVKINNQNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W8A6</accession>
    <name>A0A8S1W8A6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0850182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W8A6"/>
    <dbReference type="OrthoDB" id="10254686at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13857">
        <property type="entry name" value="Ank_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="155"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8185611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="215" mass="25308" checksum="B343D5D5D730AA82" modified="2022-10-12" version="1">MTIEEQLKQCLRSKINTSKVFNGTYLIDPTINDIKKINLKGKRQLHHLLNMEPKKKLQKKTSLLQRQSSIINPLYCRQSSIQNIEVEDVTQYMSSKILESKANIELLVERLKQPEISEKQQYFFNFVQNCDCKSLNTLFQDQYFDNSIINTPDQKGFYAIHIAIKKQNPQLLKLLLKNGARIDVLTKNGKSLKQLSELYQNQEIMEILEKYKYYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WAN2</accession>
    <name>A0A8S1WAN2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0880144</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8187094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WAN2"/>
    <dbReference type="OrthoDB" id="312685at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd14824">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15843">
        <property type="entry name" value="R-SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.50:FF:000015">
        <property type="entry name" value="Synaptobrevin 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051097">
        <property type="entry name" value="Synaptobrevin-like_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136">
        <property type="entry name" value="SNARE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136:SF168">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13774">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01270">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="191"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Longin" evidence="5">
        <location>
            <begin position="7"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="6">
        <location>
            <begin position="127"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8187094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="210" mass="24914" checksum="6AF81F4116568D58" modified="2022-10-12" version="1">MSLIYVVIVRENNITLVDYESVTSNFPSLAKKLFDKLKKNTRQTFVYDQNYHFHYINENDFTYLCLADAYLSKATAFAFLEEIKESFCQKFSENLRKQAINYGMQQQFVEYLKDKMFYYNNDPENEKIVRLKNQLTDVANQMSLTLDQILDREDQVQILVHSTQTMNKYADTMKTTATQVRKDQESRVLRTRLFIAAVIVTMFLVIYLLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WF33</accession>
    <name>A0A8S1WF33_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0880202</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8187210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WF33"/>
    <dbReference type="OrthoDB" id="414075at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02637">
        <property type="entry name" value="R3H_PARN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051181">
        <property type="entry name" value="CAF1_poly(A)_ribonucleases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034042">
        <property type="entry name" value="PARN_R3H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006941">
        <property type="entry name" value="RNase_CAF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15092">
        <property type="entry name" value="POLY A -SPECIFIC RIBONUCLEASE/TARGET OF EGR1, MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15092:SF22">
        <property type="entry name" value="POLY(A)-SPECIFIC RIBONUCLEASE PNLDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04857">
        <property type="entry name" value="CAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8187210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="501" mass="60699" checksum="D5E87FF399351BDD" modified="2022-10-12" version="1">MYIDLDNFEQNKQTIMEQIKLCDFLGFDLELTGIKGDYQDTFEELPFERYLKTRKVSMKYNIIQFGLCIFIKKQKGYVAFPYTFFLFPQDEHGMDWNRWINKGIGYQNRAQYQKLQDDYNKDFQQLDQEELKNQFEKILDDVKPIDKKTIRELIDRLEKFNKSDEKQITIETANGYQRKVIYTYLEAYQKNMIGESLLKGEMKITKLTNAEKQAKDEEKYLLKTNRFQTKTGFYQIWECILNSKKYMVGHNCFADIMFCYSHFIQCPPISWEQFKEDIVKNWPTIYDTKYIIQSAQPLEKIRSKFETSTSVENLAEFIKNNMNYIQIDVDIDKDSYHTAGFDAYMTGFIFINLTKDFTQQDLQNQKNKLNQFKSNYNINLNTVEDIKVTQKFIIYIKFPEEDRNNQQIQRNQRLQFQKQAFDNIINALKQQQYELSAFRIIHYKYIYIEFNQDYDLIQIQNTLQQVGEFQQCQLFNEKQYEKERREFKEKEAKLERFDRRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WI55</accession>
    <name>A0A8S1WI55_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0900079</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8187859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WI55"/>
    <dbReference type="OrthoDB" id="288137at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035822098" evidence="1">
        <location>
            <begin position="18"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8187859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="386" mass="44028" checksum="46752EF8B377B510" modified="2022-10-12" version="1">MTYKKLVLIALICVVASTKIQNNQGVMDQYVNPGVQQQIDELTQSKIGHLVVDLAEVTMRVTGAVDSLTEGVKAFGRQIEERLNLENTQWEAIEGEHNAKVISLSSQANQAEFDINREKKVLNSELIPRLQELDDILLGLKAKQTDNNQSFKEAEAVINTQHDSFVLTTTEFTDALRNIDEALDLLTDLFKSGEVRAAFIEISDEQKHKVNKALRQVKSNTEKFGHQYSSFIQALTNLTEGFNFKDRQVLKEMCDFLNSLRLNIFDALTKAYKDEDSQKQQDELRRQQLTSEKTVFDQQYADFYQEREDKTVRISDVQTMISTRQADLKAYQDRLRTENNNYSANLKIHDDIVSSVQQELAVLAKALQVVQTPPFLDFLNARIAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WIX5</accession>
    <name>A0A8S1WIX5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0920049</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000092">
        <property type="protein sequence ID" value="CAD8188705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WIX5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8188705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="132" mass="16143" checksum="854CA56A35FCE024" modified="2022-10-12" version="1">MSYQKDNLMNNQQMKLKNKIQKSYVIIRIQQLYYQNLTIYVKPIRVKQIYRFFILIIKIRITNNNQYYLKEPENSKKKQLYIQNKLNGKFLKNVSLLKKISSLNFVNYRINQSMESLDYNIKYESLLLDEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WN29</accession>
    <name>A0A8S1WN29_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0950019</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000095">
        <property type="protein sequence ID" value="CAD8189875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WN29"/>
    <dbReference type="OrthoDB" id="295694at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8189875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="140" mass="16547" checksum="4B2DDDB4F081C1DF" modified="2022-10-12" version="1">MFQQKEFDYSQTPEVQQLYQEFVDAFEEKLIVEDFIPVVEAACGEQCSLLLKKLQRRKNQKLDQDEFSQQFNFELSEPENFDCIYEVLEQGKGKITFEGLKAANQKYKFGLTDQDMKIMIQYTNVKDQPITKSVFRQMIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WP06</accession>
    <name>A0A8S1WP06_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0950076</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000095">
        <property type="protein sequence ID" value="CAD8189989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WP06"/>
    <dbReference type="OrthoDB" id="294006at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033337">
        <property type="entry name" value="TORTIFOLIA1/SINE1-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31355:SF31">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_74, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31355">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN TORTIFOLIA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8189989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="526" mass="60389" checksum="21AF1D4F4BB86E93" modified="2022-10-12" version="1">MSLEEFDAFTSLKALRDLKKQIEAKAQVDQKSKAALQYIQKAQPLSQYDLRIQFQHWLAKLGDLSTKQIAYTNIFQLVQQNQSQPNLQLLITCLQTPINGKGSESIIKTINIIVGIYGDTISVTDIYKFIEIMLKYFNDSNNNVQMAISECWSNIYIKNISKKPVQQRVLLMYSTLSQLIGSGGSRQTQETATIVLSQLFETLTTAQDLSFIKDVVKDYLGIYLKQQIDHSGFYTIMFYILKTLQIQSFTSQQIQVIDKTLQGINNQKLNYRTRVGALNLMKLIATQYQNSNFKIALGNLHEQVIIVLEDSTRDRVIAVQNAARTALKEWMRLKLQVDCELIPNPVSLDQSPGFLKKQSGSGGGQVYVNQKISNQQQFKQDIKQALIEDQFENTVDYSNIVHEPNLNVWQKAMRLQQDGLLEDAVELLLHEGDDLYLIRFLMSYKNYSQLFDHLSNSLALRLIDTLILILESDFLNILCLDFIQQFIDCGLGDWFKDTDFTDYLEYNTEQNLPSTPLCKKVRDQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WSW1</accession>
    <name>A0A8S1WSW1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T1000021</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I NDUFA5 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000100">
        <property type="protein sequence ID" value="CAD8191811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WSW1"/>
    <dbReference type="OrthoDB" id="286811at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006806">
        <property type="entry name" value="NDUFA5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12653:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12653">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 13 KD-B SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04716">
        <property type="entry name" value="ETC_C1_NDUFA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="174"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8191811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="189" mass="22389" checksum="FA49B2F5A5B86F98" modified="2022-10-12" version="1">MIHTLYRWMPRFRFATTLGSSNVASGNQTSATQSNPIPVYLRPYEAKKYEVPSSKIKLTTGYSFLDVEPMPRAKIMKLCYTILDKLKAEIPEGVLYRIYTEEKLKYIMRITDEIEDIRQLEEEFGFEQIEFLIQQLAKEVDLVDQMKYYKPWEKTQDDSTFELLRQPHPALKHQRNEKPPREQTKFIGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WVX3</accession>
    <name>A0A8S1WVX3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Anaphase-promoting complex subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1050133</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000105">
        <property type="protein sequence ID" value="CAD8193968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WVX3"/>
    <dbReference type="OrthoDB" id="291936at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045842">
        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037679">
        <property type="entry name" value="Apc5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12830">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12830:SF9">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0498">Mitosis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035837305" description="Anaphase-promoting complex subunit 5" evidence="5">
        <location>
            <begin position="19"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8193968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="627" mass="74086" checksum="F5F80DB4B83E4336" modified="2022-10-12" version="1">MNNNLILLILLLRKPSQQVYDILTEYLKIHDVTEIINNPTLQNTILSHIEPELLQITSMNKFHKIIGETLREKQQQKTPGGSVVDLFFRNMLLLFNQMDFNQMLKSWSDFKEGQDNHLQNNPFLTDSYIEFLLTKLQTSSSYVNLEELKQIIEQPNLNENCKVNFLKALLYAILRYPFRAIKFSRKYYDMTLNSLVPKKVNHCIMNSIYLNMKMNFYDEALNSLSEGLRLSQTVSDEQSINLCLLNLFEIAYRNNYQKQATLLLEYAVNHAQQLSPQHRLQSALIYGSQVRYKQINYSLLKKKHINWKDIIHQSLKQILQSHSQDIEQSITAYQLVCANNYGNSTMIQNALDQLQQFDQNDQTLSLQLEALSQISLHQPLYTLSQFQQCFETTYNLSEQSILVILNICLEYFEIINEPISIKYIKQIMMQLLQMNPDPYISESLQIVPIKNPKLRVKQLLKDGENDLEIIRLCKQFHLNQQYLEYKLNIIERNIKQNQIQIAQDKLNNINLYELSQSNCVIQAKFWNLMARIYKSLQYYATSINFAIQVGWIEMIQQNYYDMTFIYDQLDQYDQRDYCAEQFNKADALISQCIQNTNLIFFKINEIGIVEYLYRFLNNIQQQFSCNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WW63</accession>
    <name>A0A8S1WW63_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1020024</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000102">
        <property type="protein sequence ID" value="CAD8192535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="392073at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF8">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8192535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="354" mass="41491" checksum="4C5C6DA068D22B31" modified="2022-10-12" version="1">MKQQKQFTCQDIEHEGEDIQGFCLNLGCQDSKPQFCLQCGVDPMKHSNCKKDLKGFGYISSFITKFNQNILDLTTQLDKSFVQVKQKYEEFSKQLEKIKLQLLKISQYLNQQDYNQMKENLQLIKEGYQYLNNQEEIKKQNQIGTQLINIKQMIEAWDLNKIQQQTQYIDHQSILQQGLQLLNQQKWQQANELLIKNIKNLEKQLSFAKFFQSISLIEMNQTGIGINMRDQAKKINSNLFKDLLDYSDQELKQNPQNAFILIAKSYALNDEKQFQLAIQQCDKVLLEEPQNIHALYRKCFSLFDLKEYEQAIQCIDKALISNSKYSIGLSMKGVSLENLKKYEDXSYLNLTNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WW68</accession>
    <name>A0A8S1WW68_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1060117</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WW68"/>
    <dbReference type="OrthoDB" id="306373at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8194284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="621" mass="73761" checksum="33B5047ECAC159DE" modified="2022-10-12" version="1">MTLFFHRILDKKLEKQFTQIQNQQTITIQHNQKVLILLLLLIFAIQKGLENNWSSFAFNAFGLIFIIVSYKYVNKTSIYYKYLLLVIVELFNFFYPFNRLMKIPPNQDTYLDGYFICLGTLAIINSFDIKTRYLMLIPIFGFNLAADPHESNLFWSQVFKFSLMILLDIQYKIQQEIIKRKQFLKLSCKAIVQSFIEKEQMIETFQVQYCDDSKQIQLINQKTKQRVNEFQQIDFKQKIRNVIIRPKSKRLENLIEDNIFNFSGKLSLELFLFYFLNKTQKVNDARLVEFLKKHKQKVELDGMIDETLNQIVVYKLHHHTHPQALVIIKQLQAKTQVEQLKLKYENQNLIIKYLMNILNCSLRLCMNDAAFINQKTNLQKLSFQVQTILFKQQSQLIKAWNQFTNLIDFVQLSCNVNPLTSFNIIQLINSVIQITKNLIPENNIQIELINKLKCQQIYSNSGKVRQLFFNLIFYIFEKNPNQITITLLEEQSDLNNTFTITINYKSQIKISKSELHHFPIINPLKFKDFKHNIKNSMYLEIPISIFIVRLLGPKNKIIMRKQFQECTLQFSLYKLIQSDMQLQKALNLKSENQIIVQSEIQPIHTQYISIQHLNQQNYEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WWW4</accession>
    <name>A0A8S1WWW4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1060149</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="77931at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035811552" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="2586"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2231"/>
            <end position="2254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2266"/>
            <end position="2286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2311"/>
            <end position="2332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2387"/>
            <end position="2406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2426"/>
            <end position="2446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2452"/>
            <end position="2469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8194348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2586" mass="301854" checksum="1EF89573AB2F2AAF" modified="2022-10-12" version="1">MEKLLIFIFLCRRVLSQLDEYPKHLFIHHKNQQRLLQSECHYSCQECDGSTEQDCLSCNSGLGRYYDSIKRMCFCEKNLFDVGISECLILNEAVPSGELSQENNTIKDFCKFGQFKVLLQNNTIICLNCPKSINNNLNCLDCYLNPDSWFKQPRCTSDFLQLKNDENYGYLMQKRQEIDFVIYFISETFSLISNPYYSYLCSEQDLILGEIDCQLMDEKHINKQLYAVCKDYSFYDSNSCILCPLFCSMCNKIECIGCINHSYLYNGLCFECPQECKECQYNQNLDIVECNQCYEHFGLVQGNCIECGLNCASCMFWKFYDAYYGKWIQQLRCIQCIDPSKYFLSSNGIDCLENMLQNCLYAYQTNSLFFYYMTTYDYKFKSFLEAISGCARCKEGYDVDEDGKCVPAENLIESCLFVFKISFEEYSCLISDYSWSQQNNQCQEVISNCQECIKESWTLKLLCIQCQPGYYAHRLFGYCIQCPEELNCRICYQQQSLFQDDWVVNIRSFYEGVIDAVYVQTETTLPRTFRTYAQSQNISDYEISCKLCLDGYQLHKNICILKCPQDCLECKIENNQNICIKCQNDQYGKVLSLVDNKCLSCPSNCDLCIPRIPEEIKKINPYFENQKYQNFTNRCVKNSNSQSFAYDPDFGYFINCELKDKLCQNILTINLKLYCDQEQYNNDLEISSQTLKNKFKFLKTTLFLDDFLLNSFQQFENDQFYSLLNIKQIGKLKIRISTQQNQDCFIYNKVTIAQLFRMNVFSLSSVNLEFIGIQDTNFYIFDTLEFHNFSSISFDSINFGFKTSSIINQQTVKFVSILQQTIEFKNLKIEQEANQIQDINIQFNNIDLIYIHDVQISNLNLNSTNGLFQFISVDSSNTGTIKFENFNIYQTNLINTKIISYEISNSFMIYLENISLNLNLMNSNFFVCNQVINFGNLIINKLKLTNSVIKNTSCLIQGNFLKLIDINSIILQQNQFDHSNLILLNNNITLQNIQIENCVLLNSYLILNQLAEKNHLDTEYLFNLKNFLIINNQYNVGTKYITIYRYTSTNSKIIFEQLKIKNNIIIDTELRNQKKQQSASIILESDEIYLKNFEIFKLRGIPDFSIINSHNLTINDLYVTSQINKEKLFLQKQSLQTNDLGIVFHIYSVKQIRFQSVHLVSLFIHNYPIIYYEQATKTLINQDGQIEVYNSKFTNNLLYQSNSQFSTNLIEIFSAQKVIINFINSTFENNIIYFQTTSTSVQSTNLLNIDCSECSVNLREFNFLSNVAINTTSSTLFIKSTKISISESVFKQNGFISKDILSFLNQIEVYFDNSLFYQGSGNGVFQTDNMYVLNCNFFNQYGSKGASISFYPLNQGFFKIENTIIDISQNYFIQENEKGGVIYISSSSANLQLEIINCQFSNIYSIQGGLIMIESPQNSIQILINNTYIMDFFSLKGSLVYVNFEEQYTDNSLIQLQDLKLSNTLEGFSKFFQLYNDKNYNLQEIEASENFQIILYKRSIIFSKLGRIQIKNLIICGLINEIIIYDQYLRMLQVNSIYITNSSISNQGLIYVAPFQNKNFSIYINSVLVENLKTNTDEIQSAYYKYAKISRQNIFGIINILNIHQEGIMEFRNIKFINNQCNNCQAGLMNFQFNNYSQNNLNIQDITFKYNICGERGCLNIVQDNIYYYLIDDQDNLRLLEGEKVNNILNYVFIIQDYYCYQNLGTYGVCLFVQNLAGQIDRAIFSQNNATVSGGAVYSFGNKSNLQIMKAQIVENYAKFGGGMSIRNHFSPNLKQYETFSFENQASYFGNDYAEYPTHLIIQINNYNQFIYKQYSSLNTQQNNIKIFTATLLNNEQEIKQILLPSAQHLYLYQQFDWENNNYIMMNYTLRLIPYDQRNNRIKNLTSSKCTIKGRLYDYKKLEESEQKPFSNNFTNLNEVVYDNSMEEYNFDHLIIYFDPDLPENIVLQFEFKCDSIRIPILNENQQIDTIIKQSKEYYLRLNIKTLPCQIGELKSEEDLSCHPCDYTQDYYSVNFNSNKCKIRDNIQMEQVKSAQIKLRPNYWRPYFYADLIEYCYNFQLNCLGGWNYGDVSCSLGHIGALCEQCDIYNIRGDGAYSISQKYKCGSCDDTKSNTLIIIGISLWTLISISISVKSTVEIIKQLARASKLKSIGIMIVVTKNNAVNFIKILTNYLQIIASISTFQLQLPQNMESTINSLGNPVETMAYSLDCFLKDMFQSISIHYSRTIWQLIMPFIYILLFLQFYGVGVILKYVEFNSCVISTSLIYMFIYFQPNIIAGQIGLLSYRTISGFKWILGNVANRYDTNDHIKWLLSFCLPVLFTFGVLIPGFLFNRLYKIRQTLKDKNNRLIWGYLYNEYKESAYYWEIVKIIQKELIIIFLTFYQDQIIVKAVLVYGIIFIYNYLTLYMYPYQSMQHNILDHQSTRVCGFSIVLAIGIYGSLQSGIIEVQIPFYIIMTIINFLFLIKLILQIIKAYFQQFEVLIDNIRDFIRIRIPSLLNYPFFQKILKNQQQTRLKAQKNFRKIKLSVILLSRQIILNKKINNTQERKGTFILDSKISLVETNQNQQKSSLEEINKNFLVIGQNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WXI9</accession>
    <name>A0A8S1WXI9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T1070082</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000107">
        <property type="protein sequence ID" value="CAD8194628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WXI9"/>
    <dbReference type="OrthoDB" id="9049620at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010652">
        <property type="entry name" value="DUF1232"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038896">
        <property type="entry name" value="RNF170"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22894">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22894:SF5">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06803">
        <property type="entry name" value="DUF1232"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="169"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="63"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8194628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="230" mass="27038" checksum="1E395A5AA2CFC628" modified="2022-10-12" version="1">MYIINNVQEQIINYYKKWQNIVTQKHSLKKLCIKAKEEITQIAKQIILTMLIAQIQIETNQSCPICLNEDLIFKGVQSEQCKHSFCIACINGYWKHNQKKQLKCPCCRAKISTFAKSKKLQDEFQQECNQFILEYRVRCTVLKYNIIYPFQIIANIYKHLGQLFNLCKILLKLSIQLQLVLCFILCIYVLSPIDLFPEAIFGVLGLVDDLLCIIFIVWILITQIMIRIFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WXW4</accession>
    <name>A0A8S1WXW4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1070157</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000107">
        <property type="protein sequence ID" value="CAD8194778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WXW4"/>
    <dbReference type="OrthoDB" id="306809at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="280"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="6"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="288"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="305"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8194778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="328" mass="38735" checksum="C971D8F70ED881EA" modified="2022-10-12" version="1">MGCGVSRQQQERLKSYKQKLEELGQINDQLAKQIADLRDARNKRKPIYIIKDQEVEELTNYKGELERKFYLIEKEILRVNKQLQIQAKAEPKKKRKTTKPIDIDDIADEEEWKKNMMNDNDITIKPFDDAQLEIESNNIEKKDNNKSSQSDILMMDSQQNIKRQIAHIEQKDEINNQEQQLQFDSKKIIIQNVQQNEQNNFDNQSKLEQQDLNKIKVSIQEALGDDLQAYNDEIKNKLFSQDLSNHPQQTLKNSEIEICFNEQSLEQIVQSPERNITTIPKKSKQNQHHTSTQVQNNQQQKAEKQLFFSKQQSSNNNQQRSSKYKYQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X038</accession>
    <name>A0A8S1X038_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1090163</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000109">
        <property type="protein sequence ID" value="CAD8195523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X038"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097546">
        <property type="term" value="C:ciliary base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030992">
        <property type="term" value="C:intraciliary transport particle B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120170">
        <property type="term" value="F:intraciliary transport particle B binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035720">
        <property type="term" value="P:intraciliary anterograde transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035735">
        <property type="term" value="P:intraciliary transport involved in cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030511">
        <property type="entry name" value="TTC26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14781">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14781:SF0">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8195523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="243" mass="28806" checksum="01AEC0E042E94912" modified="2022-10-12" version="1">MNKNQGSSGRVLAVKVQLQRKRKNWAKLVSGHFEHPLFESLYQQRAQQQRQALYTSISLILTELLKIQQQFAFLFEKPLSDCIKFDISHKKKDEKNFMNFHLKISENVLDQLCLATINFLEDIMKNLQMKTESYKKIKNIPAINIYEALFDYKKDDYDVQIIILQSYQNQYPMIKIVVNLKTCKQFSLYSNKEAEEIMNLYNMLLGRKFFKTQFSIDEMRNVTSISCNFPLSQIKFNHVVFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X3U2</accession>
    <name>A0A8S1X3U2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1100077</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000110">
        <property type="protein sequence ID" value="CAD8195691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X3U2"/>
    <dbReference type="OrthoDB" id="298528at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="105"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="334"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="527"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="578"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="191"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="290"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="105"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="334"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="347"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="536"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="584"/>
            <end position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8195691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="615" mass="71277" checksum="F3D4252151ACC89D" modified="2022-10-12" version="1">MQPLFKQRKPANLIIQSSDEDKIVQQQIQELDTFEEFCSNIPDVENQELNFTKESKSFSSQPIDLQDSQEFQYNLKFSDQKQEEIQESLPIQYIEFVEFNPQSINSGQSESNIQRIDSQQSPKDIMQEPPAPQNKNLTKFSFPQKGGCFSSLKKQLCVDQPLISNHGNQSEQFEETLVKQHNTQNIPKSVQDDLLKQIKQLTDQNNDIQTTLQNQIQQLQTQNQKLEQELVQQKQQNTILIQENIMLQSKIQILEQKKSADLNNITDFSHDLLPNKLSMNEKLIYLLSKINIKAQENAKLQYEILKLQNNIEILETQKYLQINTNLLLETHKLQQSHSPQLQPQSQNRGQSLSKDSRTSLLNSQKYPLNLSLNYVYQGERRQEVFINKKNRIISPLAHYSSVGEQQQLQLTNLQKNKIYKEDTNYNNKMHTKNSTSVGELISFKQDLTNCSNMINKALRKESYSTQSPNSKVTQKFKQTLNAKIGTQQENSKSYASLLFSLGERQKDVRQMSPKGGNKKSKQLFGFIMQPHPNSRKQSEILKSEESNKMSQEQLKMSDKKNTLQTSIDIFQIMKEMRNKKNRISTKQSQKKGQATTSRQLITEIKSSRTNKPSFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X710</accession>
    <name>A0A8S1X710_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1140083</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000114">
        <property type="protein sequence ID" value="CAD8197041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X710"/>
    <dbReference type="OrthoDB" id="10310079at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="682"/>
            <end position="708"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8197041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="768" mass="90782" checksum="4DAD31673DABE736" modified="2022-10-12" version="1">MESESESPVMKKYPIENIINDASGLLKKNGNRKVILLLDKHFMSYSHNQMGTLNIQISRRLIRAAQNLLNENIQGENIQKVDLLLDRCNLYTAVIYINIQRMLKNERGTEHFEELNSAQIEQKIGLNRLRQRIQQTICIPGSLIKVTHLNRLKEKNKIKFYLNECFTLLMKQLIIYARLFKITNEVQNSLKIMHKIEKIIQIKEMKYTKNVELIKIFVDHYQNFGQLYFQLCEFKKSLKQYQIAYSYLQQLIFLILKKRNTPNVIDDSDLKPVAQYIMKIVMILYLQSLCYEYLSEYSKMKECIHLAQWFCTEILRLEDEHQLRILIFSRAQDIQKYIDNILAECEIRHMIFTFLSDDTNPEMKVIQQQVKDDYQKILNEKFYLKFKMEQLNKQQYFKLNPVPIKPPSPYLLYNVPKGSKTERELTQQQISKNYQSFTTQDGYDQHKLITSESESKLIKQNSISSFSRRTRPTDFTQYCKTEQSDYPKLDSELAGVIVNQLKKEQAFYSLVDIQRTCKDELNENQRQQQDCELGKAILLFKRQFSKNAIAEPKETDSLKQLNNEIKTEFQLISGYLEAQERLKKKRQQQQLFEQSLKPKSTIKSPFRKMLIKHSNTLGLKKTKKKLQNLEQESALMKLYQKISGQKEEQKQQQINEEQIIRQMIQTNLNAIKVIMDDNQKEQETAQKQLKSRRHISEQLPSKTEKSPKNCQFRETFKGLAGSTQYLSTKGQATKSVVSLVELANIKRKMNHLESKKVVRYSELFRSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XBW9</accession>
    <name>A0A8S1XBW9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1190165</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000119">
        <property type="protein sequence ID" value="CAD8198766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XBW9"/>
    <dbReference type="OrthoDB" id="288185at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8198766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="76" mass="8724" checksum="8C8815FAF81D16B9" modified="2022-10-12" version="1">MGCVNSNSHQKRKKDFDITNIPIMLNNQKSSNIEKIQDNWLELKNNSILARRHQQSKLSIKVTTNSKSPLSCVSRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XF41</accession>
    <name>A0A8S1XF41_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1220026</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000122">
        <property type="protein sequence ID" value="CAD8199549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XF41"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8199549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="125" mass="14996" checksum="6233A06E8D02A936" modified="2022-10-12" version="1">MKFCLFLKRSYNSIQINMGFLQLLIEVTSYQQQDQRISDNWKEYSSKLFKIGNYVLGCKLQFTFKNLFLQRNQNLSKFKSQLDITNIYFILPKDSHQREVNDFILEIFESIDLICRMITTSISRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-06-18" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XLP4</accession>
    <name>A0A8S1XLP4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1270108</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000127">
        <property type="protein sequence ID" value="CAD8201372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="4139357at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF5">
        <property type="entry name" value="MAJOR FACILITATOR-TYPE TRANSPORTER HXNZ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="23"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8201372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="143" mass="16102" checksum="57B07C6EE0144E54" modified="2022-10-12" version="1">MSEQILEQVLEQRLGFGKHYWRQFFIISFIDFLDGAEFLYLALLTPALKQEWNLSILELSIFGSSFTLGLTIGSIICGFLADKMGRKSILIIATVFQAAVVFLTVFSQNIIQMSLLRLIYGTVIGMNLAISSILMIXNSQKMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XNF7</accession>
    <name>A0A8S1XNF7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1310140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000131">
        <property type="protein sequence ID" value="CAD8202639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XNF7"/>
    <dbReference type="OrthoDB" id="309837at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="7"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8202639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="127" mass="15157" checksum="8B729BA244C5D7F4" modified="2022-10-12" version="1">MEYCDSCRAVLNERHLLEDQLARLQNKSEIVATQLQLLREENELLKSNIKVPNKQRRKNNRTKAELEQCQKEIQKLQVIIQHKDMIIQSQLNYRYGLSEEEGESLNIKMYICHILISMLESFMEFNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XPN2</accession>
    <name>A0A8S1XPN2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T1330104</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000133">
        <property type="protein sequence ID" value="CAD8203251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XPN2"/>
    <dbReference type="OrthoDB" id="289272at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="244"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="56"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8203251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="645" mass="74075" checksum="3127B1D16192E2DF" modified="2022-10-12" version="1">MRQRSGQILSPARVIQNFQMSTKNHTSKNASQSPLKFIQRNLSQIILKDLVKQDSTRKVNHGSLTERVHSQQRSNSRDKPKPQVKSSKVPTETNFPFQQISKGINLILNQKLTNKNKIILEKGSMTRRSSVEDLLTSSIQIGGKSSSIDKMPKQEISIYIHYSSEVTQNHRFHPNVTTDKILSVLKSKSGNYQVIGFATLDENIGFDYYLTIPSLPMNHMIGKTIRLKPIIGICPPKQLNLSCFQFLHVIGRGGFSTVILSRSLIDGSFVALKLISKSFVIQNEKQDLVQNERDILIEITNKGSLFTSKIEFVFETKNWIIFGIDYCPGGEMFSYMKRVQQMTESQARFYIIEVCLALGFLHHQQIIYRDLKPENVLIDITGHIQLADFGLARPNMQPEQNAYSFCGSPEYMAPEMFHNDGHNYLVDYYCLGALLYEFVTGLPPFYCEDKNIIYTRLLNEQVTFPKKLSPEVKDLIRLLMIKDPSKRLGSKNGVDDILAHPWFQDVDIAKYIQKKVDPPYIPDLTKLLFKQPTTNDRILFEQLNREQKLITQFTPMFESAFFYQQKRYKVYEFDKNSSTQVTKDTIDSLVRKRTKNSQNSQQSLLSQQSQSQLSSVICSNDTLKNTKSVTNLKFYQSLLTEPSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XSX5</accession>
    <name>A0A8S1XSX5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1340091</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000134">
        <property type="protein sequence ID" value="CAD8203622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XSX5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8203622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="62" mass="7386" checksum="F1B75CDEFBEEC2EC" modified="2022-10-12" version="1">MQQWIAIHLACTQKTQIQRNQPHKQFRLTIYKNESILFGTQLLDIKKKQLTSTTLHTLKESY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XV56</accession>
    <name>A0A8S1XV56_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T1400156</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Host cell</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000140">
        <property type="protein sequence ID" value="CAD8205446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XV56"/>
    <dbReference type="OrthoDB" id="303145at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043657">
        <property type="term" value="C:host cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051138">
        <property type="entry name" value="PIM_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22984:SF25">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22984">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="89"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8205446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="454" mass="53661" checksum="9F4C2A61388FD363" modified="2022-10-12" version="1">MLVVFYKKEVDDKNRKPRNYKLLVSCNQKILIMSIQQLSLQMNQSMKSEFNSKYISIQEISVGLKIVDPVNKVILVFQFWNNSILMDWNHFFQEFNAGNINNLFFIIEYEESKFFIYGSAEDLQCIYKFCAGKYIFRKGTQNKTLEIIKEKHDYQLKSILDYSTSMKYYEHVIMTQMNDNLDLSFYSQVTFEREKFFPLEIQIIQDLNITSTPHLIQVDALYHDGVQYSYIYSKKDLIKLSVYYNHTQLDNPCFMSHVAHQLIQLLLSLDEKGIIHPSLDLKNLYINPNSQDIVLASYYQAYYQLNNDERRICVNIGYSPPEYFQINQKLTTKANVYQVGISLFQLLLGHNPFGKSYEEMTFNHTNNNLDLNKLNAFDSVLCDFIKKLLVQDSLKRPSPQELLKHKLFSLSHKEYLSKQTMSKQYHKDIEEFVIFSQTSNIQSVKQTLKFKKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XW26</accession>
    <name>A0A8S1XW26_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1380047</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000138">
        <property type="protein sequence ID" value="CAD8204698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XW26"/>
    <dbReference type="OrthoDB" id="339325at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000810">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF68">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ATG1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="13"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8204698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="288" mass="33052" checksum="51999802409A989C" modified="2022-10-12" version="1">MFTPEQFAANYQINFSKLIGKGHDSTVFLTMNKQTKQQFALKCLAQTIEQNAINLFNEIQILRHIQHPHIITMFGYCSDCSCMLLELMTNGSLYKILLQGPLPLAIANGIIIQIAQALEYLHAKGITHGDIKLDNLLISGDYIIKVCDFGFAKINGQTPIPKTTVSGSEGYTAPEIWQIPIDLKKCDMFSLGVVYFIMITGHPPFESNNPQMEDTWWKLIKKEEWNVFWKELKLTILPEYVRIIIQKLLCVNYQMRYTADEIIQILMDKCATSDQIVDEIKKRIIQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XWB7</accession>
    <name>A0A8S1XWB7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1410142</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000141">
        <property type="protein sequence ID" value="CAD8205761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XWB7"/>
    <dbReference type="OrthoDB" id="322722at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8205761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="173" mass="20819" checksum="CAEA1D16C18C5DC6" modified="2022-10-12" version="1">MMEIIVQVLPNMILKATEIINIKQTFLKNFPKTLKEFIISKYKIQMYKSQQKMHLLGLINEVNEESEAEIQVDYDEFLEVIRKIYTTWKLLITIKSLLKFGIFWKKIRNLNNCKSIKNVARECMNNFKKYLKLIRYKRNQKLNRKEQIMQKTISIYRFSQCFSVSRLFSSGNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XYD2</accession>
    <name>A0A8S1XYD2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Casein kinase I</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1440126</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000144">
        <property type="protein sequence ID" value="CAD8206623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XYD2"/>
    <dbReference type="OrthoDB" id="300533at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001134">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="23"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8206623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="415" mass="48599" checksum="05E4555BBCAF6B43" modified="2022-10-12" version="1">MLSTSQTRSTTETISRVIVDKNYVIVKNIYKGKQYNLYLVKRQLAIKQEQSDDIDSFEHQEKYYLIKMKKQTQQNEAIPELQFLIRLKDSNCVPILINHGEQVIKGSKYHYQIMERHGPSIKLIYNYLSRNIPLPIICLIAIQALTCLEIVHKHQIVHRNLRPKKLLLSTNGNQILLSDFKYACKFKKQNGSIMINEIYKLNSNKLFLNKYSSVSQHLNQVPTPKDDLESLAYILLIYASNSNIFQIKAENKTLKLKKLESIKLSIIPEIAFKQAPLEFIHFLHLVKTSNANDYPQDYEKFKQLFRKIILSNGYTEKELSYPLFHMNFQEKTQQSQFQSQNSKFKKMQHQKMSSESIKEENADDEISLYQIEKVGEDRTIEPLIKGLAMKQFKHISYLNQPQVGSQIVKLKFTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XYD5</accession>
    <name>A0A8S1XYD5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="6">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1420107</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="6"/>
    <dbReference type="EMBL" id="CAJJDO010000142">
        <property type="protein sequence ID" value="CAD8205995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XYD5"/>
    <dbReference type="OrthoDB" id="289038at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061136">
        <property type="term" value="P:regulation of proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044635">
        <property type="entry name" value="UBP14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43982">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43982:SF6">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="3 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="8">
        <location>
            <begin position="117"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="47"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="343"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8205995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="862" mass="101328" checksum="ACD731D60F702497" modified="2022-10-12" version="1">MKSHQSLNKSFRKTPSTFEQLMRQYHSFGFKNQQILQAWQQCGFNTLTFHEELIRISQEENQNLSEEDLMNSQMHLKKQLKELEEKDLKHAIQLSIDEQNQQPQIEQQDRSSKSIPVGLKNLGNTCYMNAMLQSLFNSLPFRQLIFSIDLKQNSTLEGVFLSQLQQLFVQLQETNNSYINPLQLFEAFQKYKPGALLIKGVQNDFNELQNAFLEGIEQALKQIGEEELKKQFCQMFYGESKEILTYKEKEQEIIKFTITNFGSISIDAQDKNLEKGFLNTRILIIDEYETNSKDKVKAKIDQIILQPPKILQFYINRVYYDSKQKLPCKNNAQFEFPQQLNINQFKARDIKQMNQELQRLDIEEQKILDELTRYGDSKDDIDENFSKIIKLFKRGEGQFDQPLIIDDFQICDENTSNQQLITSQQMLFQLENYKKKFVKSIQLLTDRLEQIQLEKKLLLKSDQNMYNLTAVLMHDGGATSGHYYCYILDKNDYKTWYKCNDSLITKVDYAQVIKDATGQSRLQSNVSGLIYEQESWMKIQKIQITPKLLEYLDQERIKSQKSSDLQKIMNRKSNIIQQIQNCPLKFCKSKDPLSKELEKFSNLDLFLSVNHPNLYKYTQTLTFIQQDLNQIKDQLLLPQNIDEYIQYFITQSKLINTNIDKELAILQDEFQKINSLLQQLPLKDLTQANFQDNTQLFFLIIQQIPQKDYCIQLSKSLKDIFQVMLLKGCFLIDLSIQRKDINQGILIAQQILNFITDNKIIDDIIIKQIQENLKSSGDQAKLLSNRQDIIEQFTKLKNKSKIQIILKLMNFKSLEQNISQLQNFQNNGLQLWIEQAQTIISKQKLLIQQDRLNAEAIFYFQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XYF1</accession>
    <name>A0A8S1XYF1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Carboxypeptidase</fullName>
            <ecNumber evidence="1">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1420137</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000142">
        <property type="protein sequence ID" value="CAD8206025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XYF1"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000253">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF201">
        <property type="entry name" value="CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036515367" description="Carboxypeptidase" evidence="1">
        <location>
            <begin position="17"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8206025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="460" mass="52372" checksum="B69243680B9F6B0A" modified="2022-10-12" version="1">MMSLLITGFVIGIVYAVNPDTDRVIFPGWGEYSFNSYSGYLPIGAGLRQLSYVFLESQRNPLNDPLVVWLGGGPGCSSLLGLNEEIGPFVMVDDDREFKKNPYSWNIRANILFLESPAGVGFSINIDTKYEYNDENSGQDNYQALLVWFQAFKQFQKHKFFIAGESYAGMYIPYTAKAIVEGNKLAVFKIPLEGILIGNGLLIWDAKIRRSTLQEFFIRRNFLPPTTANTIRKICSVKPDSINCVLAETQFDQICLGSNINVYNIYGYCKEDTTPEFLKPKTKTTKQIRYPYVSWYEGNNFQDDDKRKAPCSDFGPINEYYNNATVQEALHIQERPYFWSACSQEVNKAYNISNNGSYQILPLLSQSGIKILIYSGDQDAVISVVETEQSINMIPGIQELDSWTPWGNTDKDLAGWITQYNYLKFIIVKGAGHMVPEDQRQNAFEMFNSFIYGNELPKYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XYL3</accession>
    <name>A0A8S1XYL3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1450003</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000145">
        <property type="protein sequence ID" value="CAD8206689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XYL3"/>
    <dbReference type="OrthoDB" id="303435at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd19756">
        <property type="entry name" value="Bbox2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="290"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="156"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="290"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="313"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8206689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="530" mass="61688" checksum="7E6A551B79FB3BD5" modified="2022-10-12" version="1">MNLAISSSSEEESSYNAKEQDRILKNSNIFKQKAVSHLNCLKHPNKKAKYYAQNDNRTLFCSKCALNLALKGHKIEESAVAELELQRQMRINQFQEMMKQALLVCNSKIICLNNLQISQKQQLEDQKTNCIKFFEQVIETAQQLKQTYIQKFQHDHSSLEQNILDCQQAVTNYEKQLQQYQIDIDKNHTNIVKKMEMKPFEDIMQRYEKRVRSIQQLLSEMKLESGVKINQFEQSSILTFMNKMCYNILLNDTSQSIKSRNNSINFNNPSIDMKVLDILENDDIYQSTFSNTIKDSNSQNPPSPISKLPIYKSNGSNPISTIQNSRRESNSNTPESWNHTNIQNCDQQSQINQQINSHNVSLNEQPIVINQQQQIQEKFSVKEIVKLANENAQNVEQPKNLEITPLIHQKSQSIMPQSIKIHNHQKNQYDLNTINKSQDSKQRKKQPSVDQIEQKRFYILNQNQQKSQLSQEDTLKDRIFKELSGHSSESVYNQVLKINTLQQKMKKGKENTQIDWTGSLRNKSNILAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y203</accession>
    <name>A0A8S1Y203_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cytidyltransferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1470090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000147">
        <property type="protein sequence ID" value="CAD8207445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y203"/>
    <dbReference type="OrthoDB" id="330671at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009218">
        <property type="entry name" value="HD_phosphohydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21174">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21174:SF0">
        <property type="entry name" value="HD PHOSPHOHYDROLASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="1">
        <location>
            <begin position="132"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8207445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="475" mass="56280" checksum="3C4B342D18D7B9FB" modified="2022-10-12" version="1">MKHHAKLLILCSFNSIGYQLLDQKIEEYLKTNQEEIVLYLVGTPITYLKIYEQILFHLYGRIKQYPTKFIVIFDIFNKEFRNKYQFDLVINLGTNLQGDFNIQLKINHEIQKIQFKQEGYTVNQFKRGANSGTFDHLHIGHKVLLSLSLLAVSQHLTIGITSEILLQKKKLRGFIQSYNTRVRCVKEFCQMFRPDIELQFSELIDPNGLKGQYEILITTQDCQNSIEYINNLRKQESLNEINGYIMGIIENQNHQGDGKISSTQFREQISQKNRLTEYEYLELRTEWLSVSNNLYWFESILVDYYSQPQRYYHTLRHIYDMLKQLKGIKNITNVKLATFFHDIIYYPKIHDNEEKSCLLFQQFAKEGNIDTEIVSIFILQTKSHQTDVPFLPEDDLSDLMIYLDADMSILESDAYPIYAQDIRKEYSHLSDQEYKTSRIAVLQKFLQSKIFNLRNEEKARKNIQEEIDILQNWVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y212</accession>
    <name>A0A8S1Y212_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1480118</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000148">
        <property type="protein sequence ID" value="CAD8207783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y212"/>
    <dbReference type="OrthoDB" id="295649at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF206">
        <property type="entry name" value="HSF-TYPE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="3">
        <location>
            <begin position="3"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8207783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="210" mass="25089" checksum="3DE224A9379B1189" modified="2022-10-12" version="1">MSKANKFIATLYQVLESNEYFNYVSWTNNGTSFTIHKLDEFQKIVIKDCFNSISIQSFYRQMCLYGFRLRKKKGLKEYSHPNFIQGRFKELGLVQRKRKNQAISDELIKVSNSLKDQLQKLKEQQYSFLYQLKAQYEIHKILSNKIKSLLSQFENQYPEFWKQQMRIILKLIVGCKPFYGVQTSAPLYEILKDDLLQQNDFYSPKQLPFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y2G9</accession>
    <name>A0A8S1Y2G9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T1490145</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="EMBL" id="CAJJDO010000149">
        <property type="protein sequence ID" value="CAD8208119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y2G9"/>
    <dbReference type="OrthoDB" id="272303at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF203">
        <property type="entry name" value="PALMITOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="107"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="283"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="350"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="231"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="293"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="308"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8208119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="474" mass="54921" checksum="99BB279F98CB47A1" modified="2022-10-12" version="1">MNVDIQKGREKPVRKNGLVNGLNPYQTISWIYTLIDIIIAYIFSFLFEEHGIHVLLFLIQEILLILLTIIVTTIIYTCLKATLIDPTDTIVKQEILYKKQGKEFTTDIKSYCLVCQAHVQDKTKHCWSCNKCVSLFDHHCIWLNNCVGDQNYSYFFILVIFLVILKIFKLILDIILLYYADDLLILVYICIAIDPPILIILIYLLSMHLYFKYKNITTYEYIKSKQDKKQQINQQKNPQQKQNQNESGTGYGQLLSTSKRFDLKSQLSLKTADSKLSKQNYFSNKQEDDKKNPQQSPSQPQFTQIPSLFTSKPTTPCNDQDRKQYIPQSQAKDLDEIKDKQIYNQVIVEDSEPQINESSDEEQNDNFSNNKHHCPKNSNTSQQGQIVNQQISVEIEQQIIQEKQEEVNKEIDNKSVDQDLVSVSNQQSLHAQQPPSPQNCYTFQIDLNSDQQKVIISPSSKRNSQHLIADNCTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y470</accession>
    <name>A0A8S1Y470_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1500118</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000150">
        <property type="protein sequence ID" value="CAD8208390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y470"/>
    <dbReference type="OrthoDB" id="299868at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="646"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="224"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8208390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="675" mass="80282" checksum="8CB854D3B9C513CE" modified="2022-10-12" version="1">MHKHNMHSVSAHRRIISSSIKPSISRITSAQSRSKSNNMILNQSPSGFFLEQVIPGVGEKSNVPLKVMEKYLEESYLIKNHESIVSTLKHVSGEVKKYLQYNFIYVQDLPKQYQFQDKDPKLLFRMYETLINLDQNIIQDLFDKKQQLDNSNLNQQHPKQHFRFDILIKFIEIFSELVSCIIKIIDDKAIAIFIEQIWKCLVIILDLNTQWQQDRFQSLVEVQIGGYQESLEQIKSEYKQLEEKQQMQLRAYQLKLAIEQKKNIQLREENKKICQQYNHLEDQMNELSNIEHAKGDLQNINQKLHEMDLNFIKYNKNIYESHQQASNSMKQLAKILTTKPIIPKSFIQQKYEQLNIYYKEQKPCIELMINPLISQIEIEFYHVDQINKKQLASDFLQFLFLNINAYPSQSSVEIYLLWIHADDQQFVNCRIELLNKLNQNNNNDYYINLCQQILGINTQSPSSHKVIEETQKYIKLIELTFLQQSTQNQNQQRSNLFLKQSSIIDFELEINLTNYSIILPQQLYEYIEQTCPSNIKLIEFTHQTIAFLQQQERLKWNSFAKFYLNNLNKFSYRLDLLEQFYNIDILLIWQLLIIHQRSMINKKQDEIISNEDFIERLYQLWVYHPRCLIKPQIELQLTQSNSPLAKRTQGQLSQQKSSPQIRKQGTMLSETSRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y4J2</accession>
    <name>A0A8S1Y4J2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1520017</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000152">
        <property type="protein sequence ID" value="CAD8208755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="292919at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="107"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8208755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1771" mass="213126" checksum="B92DF09C52557831" modified="2022-10-12" version="1">MKHKIPTLDLLKTTRPTSRNDSRQQSHVSFRITLQTPKTTVRYNNPQTSRIIEQSILKNDISQLKSKHRKAKTETDPLVFWDQVQNFGHLRTIEPPKFNQEKQTQLYQKLKKMKIEKEEKIRKEQEETERIKILELERKHKEQQALEQEKKRMVDSQRYQEYLAERYKIDRRFSIFTLVNALMLRKLHSKPTSFRLAKLIANQYLNLLNYKAILHKITMQQSQPLQISQILQGNAEQLAQLKNVQHRLNEGLILRRKQTRVFLNKQKTIYEQSLIPPQFSEPVLKRIRFLQACLRLKVFKLNKKNVEPKQKVKSNRVHPNRVKALKKRLDDIDFIKANSPSFNSPLSSPGQTVANPLGLFCTLYKVTDTLKNYDRIKTQKMIQKTNDYLVKRVQSLYYPVIRYVSIFYKNVVVWSIQYSDITLDRTCIVLFFKGEGRSQYFYRLDIPIGAIQKEDIGEQNCITVWPTVRDYLMNYLELKGQLALLIQTKYGCAKNYKLNFIQGLSMRSRKNITEDDINAIVETKEILNYLQTSQIVRIRKIFGSVYELLPQFQDCNLLSDTTQYILNLPLVHDTILQLRILEQQILEYRRNIYKKPQDLSTRYKDFIESNKSILNQILAFRFVHNNSMFFQDQMLEDIYDLSHKSIYIQCTTINIQDILNHYDFQICNQISKYCLISLHLTLKSQFHFDDLLFNAQKHSVRTGFQIISNFFDVDYSHFDWKFKQEYFKVRIEVTVFERDKSLKWSEKQLFQDIKQSRNERAFKPLKMNLDDFCKLGQALQETTFNNGDIQQCNFDQILKNIISEQCSYSQVADIVLKNAFTLDIKNRKIIIQPKYLHILSKTHNFIQKMDHQSLYNKIDCVFKYSVYPNEQNDIRRGYENREKENQIAQLLPFNKLFQDINQIDMRITVEARKIEQVTDYAFDFQDQWSDLHKQTMTYNKYQNFEMFQRKFLFIDQNNYMKIIYIKQHQQPIILYLYDQHLIHQISNCQRMNKYQKCQILTGIIRVLYSPTSFLYLKDHQFYNYLKVQLPNYHQIKVLLVSNSKKLLQYLDRLDYIYILKLWNNQQCYVHVKVYYLQDHKQQKGSEKIYVYDDEHLFVSVELYEFNSKLKKYILLFNKYDLELQFDVEKYFDSNIIFNWCKAIISNTKFDKRFLYRVPQIMKYNQNQSPVNLNKYMLNSFFNRQQSINVNYNEKVTRCTFNEFPKNNFDQNITDKNYLNTVHRIFRVIGHYIQKFYIIKQKNKIKIINEMQFKELKNIKLIKQEFVIISILAHTYLDMFQILYYFPKSKRKLMTYINILHFQEMDFKDHLIFNLIDNSQFLDYNDLSNKEKGNHINQKKHPQLDMNLIQKIKLIKKSEQSPFDLQSARQKKKLHSRIGFAQISNEILMKNKVIKQAINQNNQISDNVMKFLGFTRKLHNNEDNHDKLIEIQIWNRLINEVFLLNLEHDKNFNSFNTLMDRLIETFEDISDQKIKIIGKEHLMKRQSMENSRYNYKVQTELTEKFEKLIINNHVRVTQQFKFSKSRYILNTNFYDSQIMEFICSAQFRIERLGSAYVECFLEPLNNKRTDILKPLNPMPYSSSSNYNIYFRYYCLTNYFFKDIRVNLRELLNLFIADGFFKSQTNYMNSKLSISDIINMCHYLTYKIKTQNYLNLSQLALQHQETANTLRKYIITESVDQFQIDKKKDGEGYIYSEVIKKKNKHLVLLQLQLFHTQHKVIVNIFENKTDYFQQKELTFTYIQSVVPNFQFLIKTRNYFKALQRLVEVLKQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y639</accession>
    <name>A0A8S1Y639_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1560094</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000156">
        <property type="protein sequence ID" value="CAD8209876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y639"/>
    <dbReference type="OrthoDB" id="305650at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8209876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="324" mass="38668" checksum="49F4E6E3DD4AA942" modified="2022-10-12" version="1">MIPIVPFLKSFVQEGQQFFTAKDEFEKKQNELMQFILDITQSLDQVEYFDNIQEELYTNEEKQFIKQCKDLLHLSEQLVQQLPSNLTLINGYLYSKTSHYKELLHHLEELNKISSQIRQLELQSYEVLKTVIVPSLSRKVSFQSMHEVSIFLKLKAPKEIKEIFNQYPRHLKSEYTFKIHIDDNDHSKNVYNFSRKEFMKLQQIKKDAQIYTISKKQHCTLELRNIQQNQFIKQKSQRLETQFDTPLHKELDQEQDNKTQILRLYLIVNGQNGINIIRDQNQRIYVGCGEEFGLQEKDEIIILSNSNQETLISYIIQRFIIKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YAF1</accession>
    <name>A0A8S1YAF1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1510006</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000151">
        <property type="protein sequence ID" value="CAD8208454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YAF1"/>
    <dbReference type="OrthoDB" id="312927at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8208454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="397" mass="47337" checksum="78410CA2B9A9EE04" modified="2022-10-12" version="1">MNNQVHFSFKSFKVIDLLEDSLTYYSKRISLIYNIDKFSQIILNPKSITQLKKNQEQTLQILFSKKHKKEDLEFKINCYDPQIKKQISYEIKINLKSSKIMDIFGKTPAHRLIRYYKKSISQQAKQMDIVKVNQKSLKNLDIINFSLENQILCLKTAFICEVLNEKICKNQLRKSSSLNHNQNMYIKEMLQISQTIKKSVQAEKQDQLFGIKSLMQQLPLVRRIKMDSHEEEDTQKINYQDLLNISNNKQNINDSEELILSKKCLKMEEREVSNVLQSQKYFKRISKCYQIQNQLTACKQMVVSSQKHILKINQNQKIYIGQQWFPFYLKLFCQDSKSPWQLFNKAINYLQKKGVNYLLKKERAFQIMKNFLTMQKLEIQNQIIFQTNKLIKIILFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YIN6</accession>
    <name>A0A8S1YIN6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1870007</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000187">
        <property type="protein sequence ID" value="CAD8213810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="298777at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1063" mass="122288" checksum="1BD44ADDCDF27FF0" modified="2022-10-12" version="1">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</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YMP8</accession>
    <name>A0A8S1YMP8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1700008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000170">
        <property type="protein sequence ID" value="CAD8212632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YMP8"/>
    <dbReference type="OrthoDB" id="6262491at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="280"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="329"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="375"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8212632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="557" mass="65537" checksum="3BC22747D9FAAD19" modified="2022-10-12" version="1">MKKCKTIETQEMLQCSNKHNNCPVIVILLDKDLEGAQRLVCQQCIVNNLGFINGISITQGLCRIQSIKEQIFDSISIHFQLAYDKLEAFQGQIQQVKRDFLQSINQITENLDLWMKQFVTLKEGYEQYSLLAEIDILNKPSDQLIELEKQKFKDKIKTITDQYWTKIFQNIQALERTSKDQKLLFQLNQLQDLNLNLETIQSVENVQNEQQQKESNQSTISQVDKQNRVQSQLIQEVNQEQQCHVLDFNNDNSLIAASCDKNIKIWKFRDGQMIDQNIVLTGHESDVFCILFSKKINWIVSGDKNNHIRSWIQKQGSFGATQWESSQPLTQHKSFILCLYLNQNEDELISGSGDQTIKVWRVNCQSNKIEFLYSLCKHNGYVDKVNLNSKETQMVSCGWDKQIILWEKDGSNKWQFKYILTQSIQDIGIRIGYLEDDTIVWCQLEKPFLHVFKSQNGKFQERSDLKVQLKEIIPENNFFDYNLFQLKYLSKEKVLIFKSNRYLYFMKQSLNSQLIYLCNPIDLQTIESYGNITNDGKYLVNWNKKCKQFLIYKFEYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YP46</accession>
    <name>A0A8S1YP46_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1720067</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000172">
        <property type="protein sequence ID" value="CAD8212994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YP46"/>
    <dbReference type="OrthoDB" id="293654at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011:SF28">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="234"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="394"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8212994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="636" mass="75159" checksum="D55FF239F9206B50" modified="2022-10-12" version="1">MYNILRVKNAFKQNRNKTPQESANQRQNSQSFYDQSNYSLSVTKSKTNNSTTINTINRTINSFYLGPRREKLQSYTIELQKTMDVDFGNKQRTGFRESGRLSRKLHTSYGPQQQRPISHSKSVIYGKKEMENNDEQIQENITIIQQGLIQEPDKPLINSNEELSKIKEQVNSMYKNLEPDTMEVDQWDYQVDYEKLELNVFSLDELRYLIQEFEKEEYERNYLTMDKILLKNKFFQRFGQGIRIGIMEKASMEIHDVGKVILQLGANSNNLYVILRGSCQQTLTMDIQLQEYEYLTVQSYFDGQDLSEINLLQLNNSRGYSANKFIDMVEEIKEHPQLKDLLKNERTKLITSHISCGEQTYLLRMDNDQFQKILKQSIEKDKEFKLSILSQIRFFQHTSASQLLHLVAELGIQKYFQGDCVVRKGDQLNRVIIIAQGEFEIVEEIQLKRETRNYYLENKLKPLVHKKIIDKNTKPILPRDFADQSFDLSLNNNQNQQVIRNSKCYQYCNKQIRGKNEISYTHLHVLKTLKKCDVICGRSLLVLYDNEYDQEQTSNAKLTVVVKSVQGSVFFLDEKRYQNLPESLQNQILAGLRSMKEFDDYEIDHIRKQIKTWEKYKQNLYQQFILEKRQNTFKTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RVW1</accession>
    <name>A0A8S1RVW1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0010148</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RVW1"/>
    <dbReference type="OrthoDB" id="304712at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="47"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="431"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="730"/>
            <end position="789"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8131550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="845" mass="101896" checksum="5ECA9919B3CA13F2" modified="2022-10-12" version="1">MYDARQTYLDKDSSLHNISNLSDKKDYHFYQRDLLAELNHSQQRLTVQELENKVVMLATENERLLSQLSDKNREIELLQEEQRMLQNAYQQQKDERYRTIENRTTKMIYENEKVVLMNQSLQIQLEELSYQYESLQKKFQVETEIIEQKWKAHYGELYEQQNKNLTANIEKLLNELKQNSDLIQSYEEKLKSYSKELQIAKEQIDYKDKKGNELLNNNQQITNIIEQQDAVIKKNTEKIQQLQIQLEQQINENRQQKQKLINYEKDIENRRIKYEELIIQLEELKLLEKEERLQLEKNEQEFQQYKIEINQMKSNLNELNRNHKLQNQENQHNYNKEINNFKKQVQQVIMENEDLQKLLNQTRQNLNHEITKTNNLEKQLADNAQEIITKEQQLLMQYKELEAQKLKFDKLFQENTSYLKQQQNQFDIKLLQNQQQNKLQTEQILQQKNKEINNLNEKINVLLQKIEEQTSYIQQLAYQSNMKGEEIEETRPQIQSLRNELHRLQQLTVEQSNEIDNWKLKTIRMEENYEMQNKDQLEKLQRFQDEIQQLQTKLNKLQEENIKITNNLQETLSNLNQIQQLLSETHQQLKFAENKIEIQSQELVETIQLREQLNLLKEKHQKEIEALKKAVDSSLKVKIEKEIAQEREKLENYSQQITLEKRGLENIIQNLNTENQLLKTEIMQQQEYFKLQVQDLIVDQQKHLINIDHVYKLELKGLSEAQQIQYNSFVAQLHSQIATLIEENKNLKEQCQQWQQQLKKSEKQSSKLITEMECRVQSLKKDNSRLIDEKCYEKSKNRFLETQIKRSTPVREVRSSSTYVIGTPNRNRNGRQSVLREIDLINHYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RWX8</accession>
    <name>A0A8S1RWX8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0010337</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RWX8"/>
    <dbReference type="OrthoDB" id="283398at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000154">
        <property type="entry name" value="Alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051044">
        <property type="entry name" value="MAG_DAG_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11614">
        <property type="entry name" value="PHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="67"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8131890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="317" mass="36421" checksum="05BE7CC79C0AADA2" modified="2022-10-12" version="1">MYWFKKSQKDIKNESIQKAVPEEKYRMPCPLGNSKWDEEGVIQFTCLQRNTNIKLHTYRCFTKSNQTPQSVTLFFHGLNEHLGLYAHIAQALSKEANSVCVGFDFRGFGKSEGLRGWLESKEQHIEDCTRFIQLIKQLYPGVQLFALGQSLGGLTSYLLGMNDLVDGTILITPALMDNYYNRPYLKKIALILGLLSPTWSPFPASYPNASKNPQVLEDNLKDPYINWNSTLPGTARVLLKMMRDAPNTFKNYKKPFLIISGGMDQIVDPDVGHELMKLSTSTDKELIYYENMWHDCIAEQEILEIIPQLIKWIKKRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S4D6</accession>
    <name>A0A8S1S4D6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0030289</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S4D6"/>
    <dbReference type="OrthoDB" id="301464at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8134402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="102" mass="11854" checksum="A2DA3F3B473D0531" modified="2022-10-12" version="1">MNDSMGSLSTNDSSEGQERKKKKNNAILKKTTRPINISYNEVVQLLNPQNLFINKNLEESFNVIQIIHQSSYFPNFKFIYDENLVCSEIKWDRLPTLVKLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S6B7</accession>
    <name>A0A8S1S6B7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0040070</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S6B7"/>
    <dbReference type="OrthoDB" id="307079at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8135133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="194" mass="23391" checksum="0B0C6DB808C29BC8" modified="2022-10-12" version="1">MSKLFKYKKVGNSMMVITKDSIELMEQLRTEHTNLCKSISELDMKRQLIEDKMRELSIKLNSFYQHSDQPYIPKLIYKFPCDSQQDQRTIDDEEVIFQINDYQEFIPDFQENDTKTKKELFNYFNYKQDEKDVFNDLNNTNNLKKLAEEFGIKSNNINKMKQQIKIIQNYLENQSFPSSWNNIYNTELFDKLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S889</accession>
    <name>A0A8S1S889_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0050063</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S889"/>
    <dbReference type="OrthoDB" id="2365600at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012962">
        <property type="entry name" value="Pept_M54_archaemetzincn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15910">
        <property type="entry name" value="ARCHAEMETZINCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15910:SF1">
        <property type="entry name" value="ARCHAEMETZINCIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8136268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="279" mass="32753" checksum="29B7421F2E0E649C" modified="2022-10-12" version="1">MKNKQKLIKFEQKIYSKSLKIEIDNQIENSMKEEQLQTLKIISQNNEGLMPQKNKLIKSELEPIGLYNLSLEKSINIQTIKSWIEIYLGLEVKILNLQLALYYEKNDFYIYDIDHNQTYKLEKDDKDIINVLSIFKVMIRVKPKNIQSFIVIVDQDIYDPYQPEANILGRAGNQKVCVVQVQTELNDFYSTIVHELLHTLGFGHCKDQDCLMYANISTSMQLCQKHLQKLSSKKEVEELKRYKALLHISKQIGFHNECQKLEKLIKKVETQKDTLLQII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S8G5</accession>
    <name>A0A8S1S8G5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0050204</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S8G5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF150">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="321"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8136546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1078" mass="126823" checksum="3B575AA2CA3A07F9" modified="2022-10-12" version="1">MTIIHRFISYCYLWLPKQIQKSVQKTLFQQQENGQVYKIEFSNGEVQKIINFENTINYIQFVGSFLVIKKVIDLIKRVENQILLILLVIALSGKTYYQDFTEQIIVFYSLICYFLDKLQYLVNLLLISHKLRKQQSIVNNSKCRIFSKFPNISDEEIQKMEEKNQDYLEFKSYKSIKWSELQLGDIVCLKRGKQSPADLLILDSSQEELLVDFNVRTACSCTFVNINQNTKGNIMDFITKLNGQISFSIQESPVGSIKLKNDPKATSFNIKNMIMKGETLERVDWIFGMAIRVGDDCCYVQTNVKKQNYSLKSWLVEFQTYICYFCWILFSICFISNMVYSSFFLDEYFFKSLTYCLLIFPQNLLLLEKICYFFIIIKNNESFYKEQQKVKNKDKVKKQQKLRINDYPVVLKSQNDRKILMPIEKYFNLPLLSFQRSTQLLGFKSHNQNQTGFMILTSQNILDLIKTDIIILDNPKFLFKVKPKVVLLVENFKIYHFNHNKLKNLIKNASPKQKTNCDKLLIDTNRQQTQDEMKTLDIDLLISEKRVPDLRNCSDILFASNQIQKIGNLKEKQQIDQKQNDTKLNNGLGKKKSARYIFDSSFVKHQQNSNQIFDNSKDASNHNISQSFQSSQIGLQKQRSQFIKPGTIIKQNPNFIQQSQKRINDLNSDRIISENYNEQDFIDILYSQDDRIYNEILVMMLLTNSIVSVFNEKLNKLEFIFENRYDESILQFCELLDYSLICSTEQENNRQEINSRTIIKKVISIGSSSKVFEVLTFLEPTENRKHTLSVLVRDPESFLLDEGALLYTRIETNNQSENKYHENLQEMSWDGLKTFLFQKRQLGYAQTNDILKKLSAISETYGNRSHEIEKLFIELESKSEPIFTIGISSTKQKLLQNSSQELIQDQLNQERIFFTLQNYNIKLCIIIPDSFDDLMLFLRTYSIIQTKDQIIEFKEKDSQQLQYKFRQHLQNLMSYNISNYNDNFIIVSSEAFDSILKDEYLKYHFVFIFQMSGGLGAYQFTSRQKGKLAKILSKINTKILSVGNSLDDEYLFTQLKKNRKYQCQILNMQLQIQNNYQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S8U9</accession>
    <name>A0A8S1S8U9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0040244</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S8U9"/>
    <dbReference type="OrthoDB" id="289292at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8135479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="121" mass="14258" checksum="60AB21494211887E" modified="2022-10-12" version="1">MNFMNTHSQFNFSRKSLPRPLISRQLTREKSQPSFNPITPSTKFRISNTNQQMNREKIQKANGNYFSQHDDTSGSTKMSKAHTQQLLKKNEIEFKQSLFDQEDKEEELQFTLLCQTLEKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S8X2</accession>
    <name>A0A8S1S8X2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ABC1 atypical kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0040254</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S8X2"/>
    <dbReference type="OrthoDB" id="427480at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052402">
        <property type="entry name" value="ADCK_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45890:SF1">
        <property type="entry name" value="AARF DOMAIN CONTAINING KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45890">
        <property type="entry name" value="AARF DOMAIN CONTAINING KINASE 2 (PREDICTED)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="2">
        <location>
            <begin position="159"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8135499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="591" mass="68845" checksum="C1F44D947156B322" modified="2022-10-12" version="1">MQKILKYPFTHFNKPQPFFTKTKIGLSLGVGFSYILYRQGRERYQQMHFSSMYNQIPDTEALTQEYFGEKNTKQLSFFQRIIQSLRRKIRFLHLLLKFLPLTIFLPFSLIFKSYLFPYWLKYLVYTLQSAGPLWIKLGQWASHRGDIFGEEIIAALESLRDDTSPHSLEKTNQQFEQAFKKKIKDVFDEFEEKPIASASIAQVHRAKLNGEYVAIKVRHPDIIDNLVMDIRILYKVANFFSETLKIKQLGMPVTFEEFQKTLVNQTDLRFEGRNLKVFCDKFKNNPHVVFPRPIEEYVSADILTETYEDAIPLTLFLKQKRTLEHLALANLGLKAFYKMLIYDNFIHADCHAGNIMIRIKDFPVPKTLKEKVQQKLWNLQDSVYEIVDGLIEKGLTKIAEYQTSQTLDQGNMLKLKQEKKTDEKLFDTYLRGQGQTRKDVQIIFLDPGMITILNKSDRASFIKLVMFVTLRKPYECGKLMLQLAHYNNRQIEQKVQDKFMKEMQDLFTNVCKVPLAQMNIGQVLRSMLEILRANGMSIEGHFAALLTNMIVLEGLAKALDPDLNIVAKAASFIIHIRTIDKTIDEIIQQAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S991</accession>
    <name>A0A8S1S991_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thioredoxin-like fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0050365</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S991"/>
    <dbReference type="OrthoDB" id="289091at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02972">
        <property type="entry name" value="DsbA_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33875:SF2">
        <property type="entry name" value="ACR183CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33875">
        <property type="entry name" value="OS09G0542200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13462">
        <property type="entry name" value="Thioredoxin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035712264" description="Thioredoxin-like fold domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin-like fold" evidence="2">
        <location>
            <begin position="29"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8136826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="214" mass="24573" checksum="1B5B073BFEB272CE" modified="2022-10-12" version="1">MTKVFVLISLFLGICLGSQYTPIPKIPDGLIIGDNPNLVIEAYYDIFCPGSRESYNIFKTVIESLEKDSFTFIIHQFPLPYHKNAFSASAGYKYIWKTISSEAAYKFEGLMLNNLEQFTDLATLNLKQSEVYQKIIDLVKTQLPQYQINYDELLNSMKPGTPENIETRYSWKYGTSRSVSGTPTIFVNGVLFDKGEELSAQQWIQYIQNRKQQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SB60</accession>
    <name>A0A8S1SB60_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutaredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0050336</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SB60"/>
    <dbReference type="OrthoDB" id="415696at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03028">
        <property type="entry name" value="GRX_PICOT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033658">
        <property type="entry name" value="GRX_PICOT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004480">
        <property type="entry name" value="Monothiol_GRX-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293">
        <property type="entry name" value="GLUTAREDOXIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293:SF72">
        <property type="entry name" value="MONOTHIOL GLUTAREDOXIN-S14, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="6">
        <location>
            <begin position="138"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8136767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="310" mass="36464" checksum="F58123EDF30B0FCC" modified="2022-10-12" version="1">MTTITQEYSLNAFQQSNFHKIIITVFYHDNEYKTRLDKLLQITPLDIDKYALSYCNDEQIARTHNIQQLPSILISSYPYLISNQILNLEPAQFVVQLDSIFEQFEAEFKNQQQHYFLKIEQMIKENPIMLFIVGTPEKPMCRFTSKLIQKLQPFGIQFGFYDIEIDKLIVGYLKLYSKWQTFPQVFVNGKLIGGADATVELIEQKKFQELIPKEAYMTKNSKLEQIQLISKYIILIDGQPDLNCEIARIMTQKKIPFQFYNIGMDQEVRSEVLNFMSSYIETSHDLEPLLLYKNTIYNEITLKKELSILQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SCZ6</accession>
    <name>A0A8S1SCZ6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0050418</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SCZ6"/>
    <dbReference type="OrthoDB" id="387430at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 5-kinase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8136932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="248" mass="28602" checksum="5835CF57A837B2D9" modified="2022-10-12" version="1">MAQLNRPIECIMKLHDIVNKECKMIQRKKFINSYIQEKALQNFYQENVLDYKMAPKKHSLIRGTMFTKEFMELENKNKYYYGEIHKGVPYGQGLLIEIDDQKIEEGIWIDGHLSWGQRSIQLKGVPSIVCGEMIDGVINGLGKLICKDGDSYTGEWINGQKHGMGIYKYANGDMYKGEFKYDLKDGQGTYKAYNGDIYIGSFEKGIKNGDGKYISKNEDTYIGKFKDDKIDGDVNKECIRMVIQLEYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SH72</accession>
    <name>A0A8S1SH72_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">guanylate cyclase</fullName>
            <ecNumber evidence="4">4.6.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Guanylyl cyclase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPENT_87.1.T0070476</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP = 3',5'-cyclic GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13665"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="EC" id="4.6.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">In the C-terminal section; belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">In the N-terminal section; belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.2" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8139110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="354346at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004383">
        <property type="term" value="F:guanylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000026">
        <property type="entry name" value="Guanylyl cyclase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000045">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000357">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF150">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0141">cGMP biosynthesis</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="304"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="350"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1010"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1043"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1092"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1131"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1160"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1183"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1298"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1325"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1351"/>
            <end position="1369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1375"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1401"/>
            <end position="1422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1437"/>
            <end position="1461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1851"/>
            <end position="1875"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1881"/>
            <end position="1899"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1906"/>
            <end position="1928"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1958"/>
            <end position="1977"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1983"/>
            <end position="2002"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="16">
        <location>
            <begin position="1515"/>
            <end position="1650"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="16">
        <location>
            <begin position="2060"/>
            <end position="2191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00061677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00075861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8139110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2269" mass="263943" checksum="AA0B91DF3CD246C0" modified="2022-10-12" version="1">MFYSNERAEKLVEWFRQFIQNFYSQNAQMITMEMNKGILTLLSVPQEQNVFCSQEYTVVNFIPKTFLRCFMRISNVFLLTISLIMIINPSLSPYNNWIIIVQIAAGMIVFICNEALCDWQRRNSDKLVNQQSTKRGGRNGVIEEIKWQDINIGDVLILNKGDIVPADIIVLDTGQVRDREAVCMVDSSYCDGKSTFTKKKSCYLTQLIVLRTRQKSQFAEYRKLLTGRLEYEVPNRHANIFRGRLKLKKDPKVEFLSIENLILQGSTIKQTSWLFGLVVYVGSKTKNSMSSKKNKAKCSHEENLIDFISLFMITLILFFCLISIIVLLARSDDLTFVLRIDKNTQNGMKIFNLLILYSQFIPANIFMILDLINIVNKFRFKLQRPQTFSELGSLDYMLLDKTGTVTTSYYKLDNFQFGSQTFNLNHDQLFNQLTKNKRIDEEEDVVKFASIFDQEYYIPMEYDTVNRKTSQVIDLMPNINKKVLKSVTTDNNQQIFNQVLEQQYQLQPNSVRSQDFHDMISLRKSMLFQHKKRNLLHQHQNLMNNNNDEFLQMINQYKHKSPQLEYSELQNENALYYDCFMKCLILCHEARPVYLNDVQYESFSKNEEIALGFARLCGYQLESFNKFDSPDAYLCKIRNQKIQYDILGLNQYTENRNVNSIVVQSQKSGDIGDWDSCAISHLSGEGSKNKSLLICKGDYDAIKTKLQLTPKEKEELEQHIQILKMRGVRMILYGTRVLNEKETEEYKKQFNLLKNSLTNQDDQLEQLAIQYEQQLSIIGMIGFKEELKQDAHEFIQATKSQNIHIWLLSGDQEAQTISCAQALQINESIYNKYLQISQTENEKIWFQLNTCIGIIQSEMVKQQLCNEDFNTLINSSNTIYDGINYQKMLKFTLLVNGNALSVIQGDNDLMSHFRFLAGICKNVIGYNMNPQHKEMMCKIIRNSLNKHVIAVGDGFNDQLMMQYSNISIEVINNKCKQSIINTGDIKVQYLGEIKELLLQGKIYQEKLHHLVLYCFYAAGLLGMSLFFFNWFCYFTSTSLHQSLIIFFYVQIFIGINALLIGLFSKQTSYYINQLYPSLYIDGQIRVKIIWKLFLLNMIESFLTAASIFYMITYQFNYAINDQGQIIDLQMNSLGIIYCFIIVIIFRVFQGLNTFGKLMQLKLIIILILIIIFSDEYQNETYQIFTRYQCIISIIISLFLSFTIKYIFQDCIRLRIFPNMNRIVNQLTVNQQINISHIISQVFKDCKVLSPYIQEILNPGDTKITEMRLSKLTLKINDLIVEQKFLSIKLQESLNNYRLFLILLLIYFGIYCFIDYFISLQRLQTGVQYLSMFCIFVIIITYIMSKHFQQNYYTHSHMIFIVFYIIKIALDWLSDDLSFIMSSVLVVLFTTNNTMNINIIPIFVYNIIYLIQLIVRIVVVLIADSSLPTLGYYNHTRVVIYGSSTSIILMLSLTFSLLYTLYKTIQHRRTDFLAKYQIEQDNLAANDILSILVPKFVRNQIQLGSLHMQEAQNDVSILFCYICDFDSIMKEEGRNVVLMLDSLFRIFDNLCLQHGVQKIETVGYTYMAATGIKASEINISPHMLKTEKTMRLVNMAFDMMEQIKGRQYGKGNQIEMKIGIHVGRVIAGLIGHHKPQFSLIGDPVNQTSRVGSTGDVGAITLSAEAFKQAKQGIKYYHKKQKEAKGLGLIDTYQVFKIKPNNYQIPINQLRWQQLAKLVIKQQKISRNLAGEVQKETFLQKIQNSLNNLHDNSWGSQKISNSPKTQFAQSLMNVPSLPDQRRHSQLDTIPIQSLAILDSEETQIHDQQHVQDDENKFKGLLKPNIFLMIPESQSLIKNSFYNIHYEQQQYESYFGLLTLWIIYFVITLLSIIIRQFFGQSYDIFFIRSLFLLCLIILLPIIKQTYKNIYINWIFYVVFLYGSFAIVYGTFLANNQLVSTISMVELIYLVVVVCSLKIFTFVQILMFMILVFTSFLALFIDNNQITHQYIFFLLSSIVLCLIGYYKGMSELIEMYNNLQLNEQRKAKQISLVSQLLPTHSYLKMKNNTLYNRSEFIDDLDDVTLLFADIKGFTEYSHTQTPEGVVTMLRNLYTEFDKLCLQYNVYKMYTIGDCYVVMGFQQASKRNPIQEAINTVKMGFSMIDIIMEVRKLIDFPLLNMRIGIHTGSIIGGIIGTDIVRYDIYGQDVSIANKMESSGSEGHVQISETTKLMIERAERNPFVFQFKQNVELAKFNTSINGYLVEWERKNRDRGESHFRVEPYIARDQKHSQNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SMF6</accession>
    <name>A0A8S1SMF6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0090210</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SMF6"/>
    <dbReference type="OrthoDB" id="300907at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802:SF155">
        <property type="entry name" value="DEUBIQUITINASE MYSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802">
        <property type="entry name" value="SWI/SNF COMPLEX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="75"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8140646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="154" mass="17916" checksum="F278661BF524965C" modified="2022-10-12" version="1">MEEENNFKDSLNNELTNTKSPKSKKIKKIDSLSPKKNVGHWTNEEHEKYLKFLEDNTLMKKNNKIFKPMSEIIGTRSPSQCRSHHQKFNPQSPLGQRKSSKIISVSNQATPATTLYEETNATQEDEMINNNNRIKLMFFEEDDIVQPNFNLDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SNU8</accession>
    <name>A0A8S1SNU8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0110118</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SNU8"/>
    <dbReference type="OrthoDB" id="313382at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="109"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="178"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8142395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="427" mass="49963" checksum="313D2E69B98C1448" modified="2022-10-12" version="1">MHQQPRLSPVSSSPLHQNQIITQQINRQQCYQQTISNSKISQTKNKIFQDSQLQLKERDNLIKQQLFSKGLKQQQQNDLQSKSNSIQISQTNQNTNSNLNLLQCTSYFDDALKNQKEEFLKLIEQQNKQIEILHQQQQIILHQQRMKQQDYETQLCQIKNQLKQQINSKCKEIFNKIQKQQQSDFQLLEAEIAKLSQLINQSSNLTKIKLSSSGKENQLQQLQDKQIQHLEYNNNSLVNSDLYIKKFNFESINQKTNDLKLPEHISVLLNSNNQSNSEIQEIELIQSELTLFNASNQLKQDIIQENMPSPLKTIITKRKDQQGFDKQDSPFKNLNDQDMLDSLDDIIVQDSTQQQQLLSNKNEFNLLKSQGMSVIQEDEDDDEELIYQIDENGFILTQDGHPLIDEKGKRIQLTKQEMQFYKVQTLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SQG3</accession>
    <name>A0A8S1SQG3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ligand-effect modulator 3 LEM3 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0090259</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the CDC50/LEM3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SQG3"/>
    <dbReference type="OrthoDB" id="340608at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005045">
        <property type="entry name" value="CDC50/LEM3_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10926:SF0">
        <property type="entry name" value="CDC50, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10926">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03381">
        <property type="entry name" value="CDC50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015840">
        <property type="entry name" value="DUF284_TM_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="260"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR015840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8140744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="293" mass="34484" checksum="36E40A07D76315F4" modified="2022-10-12" version="1">MAEYSNSFSKQFREDQEEFLNIAIKKWKFHKNFKRNLIIFSIIVEKEVYYGSSCTTNQLNCEIPIEISSDMTAPIFVYYQLENFYRRNRNYFKSKSVEQLKGNVDADLSNCGDYQTNSDMEKVKSYGGNTLNKSENAFPCGEIAYTYFTDTFKLKDSKGQFVEIDETDIAWESDREYNFKNPKGWEKFAWTNIEDEHFMVWMRTAGQGRLKKLWGRIQNDLSKGQYILIVNNTYEEQLYSSDMVKSFFMTTTTIFGQKNMVLVGSYFAGAFICLCSIIVLVVIYFRDKRRKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T394</accession>
    <name>A0A8S1T394_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0140246</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T394"/>
    <dbReference type="OrthoDB" id="10252009at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000128">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF511">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="7">
        <location>
            <begin position="17"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="8">
        <location>
            <begin position="78"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="160"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="187"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8145444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="236" mass="27342" checksum="F9E71863F401B8E2" modified="2022-10-12" version="1">MIQQLNKWDCILEPILKNNVYIAGLYLADIHCALSLDLLQSLQIGALLSVIDEPKIDASAYIIHEVINIPDCTQQNIQDYFPQTNQFIEQHRQHTNVMVHCFAGISRSASVIIAYLMFKFQWGFQTALNYVVSKRPQVKPNFGFIQQLIQYEKQNKIRQSSQKHQVQGSISNKTEKNLSQSDSKSNRQQLNSKLDLNQLEVEIAQRQQELQILKLQYQQLNEKIISRNTKNIESLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T4I6</accession>
    <name>A0A8S1T4I6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0140414</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T4I6"/>
    <dbReference type="OrthoDB" id="10639313at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8145754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="195" mass="22258" checksum="3E670EB8443725A2" modified="2022-10-12" version="1">MTTLQIKNLTFINNIMHEYIQDNLKVSALVFNIDCQQASVILIKSQLSGNMVFNSTNSLIMIKGKSLIMSDCQFFNNSIFDYSIIQPHILLGFSDQDQIYLEDIQKMFQIKSSFENAQFIMEELKIKNCKFQNSQGSYGGAFQVIAQNYCNIQLINLSFKNVMTAFQEENEQGGSLFIDSSASQSLNLTIRDYQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T6G0</accession>
    <name>A0A8S1T6G0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0170289</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8148080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T6G0"/>
    <dbReference type="OrthoDB" id="298166at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8148080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="101" mass="11692" checksum="69AF845C365C3237" modified="2022-10-12" version="1">MSDSLRSLSTNQSIEGQISKKKKVQSTSSKTNRPQNISYSELIDLLHPNHLFINISLEDKFNAIEIIDSTYFPNFKFVVDENLEDTEVKWKRLPSFIKLTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T738</accession>
    <name>A0A8S1T738_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0160085</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8146854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T738"/>
    <dbReference type="OrthoDB" id="306846at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="101"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="248"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="366"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="574"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="102"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8146854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="824" mass="97585" checksum="4F50CEA869C73882" modified="2022-10-12" version="1">MQQQQNQSQNASKIYKKASTNSNCQTDQKYLSPRTTESTNRKISHIYEEEFRQRVDSILHENHQQLNLGNQLLKQLEDIKQQQVDKNEEILRAIKFYKNFHTHSPQRQSSTEQRSISPKSKNPNVQQLQKGFQNPDVKDVFDKLSQYKFVELNYFLEELQESDKQTPSLAYKKIHNKDLNSLPEQIKYFSPSYLKSILDYSKSQANSIKQHIQQVIETSMDKIHIKKPSLKQSSKYADINWQKIDQNFKDQSTLINELQHEVSLLQNQITILQKDFGSIQSQQQTNIEQTDEKINKISNDILQLQENTNSITYQQDNTKKSSTISRQSISNLSETQIKRTTKQKQTDLVEKHAMLSYFRAEFVTNFDEINQKFQQMLEDNKKVLEERINNLSQEFKNLTEAINYTQILQNISSSVEPSNNQDMIQKSCTERAQEIIDNESITNIKSQQESQNQNQEKVQDQSITQEIDTLKTQNDVLIKQINSMHEDIQRLNRWIKEQQNFNQLYQTKFEKRLSQVQSDHEIMQQEQEQCKIIMTTFKNSMRIDAIQNSENSFRSDQEQILQKQYSEKNIHSNIQQLDLKVQELTIQVDKLDNKLQKFMENKTTKNISINDILIYLHQQQHVTNICIISEKKDFITLGINSNEIKITTYIKVYDNSFFTAFAYQFLKNKIMYEQDDFYSFIEDIKQLNFIVYCDQLNISQSQFGSLKQLLILYLIQIYNEDQDQRIQAFNQLYKNQNNLNFHILANIFIRNMFHSKVQSTLKILNWNTEIDDTLNLQSLSQQLNINLHFIQVCNTEFKKNIFEQGKDKIGILDHNKQYYIINLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T8A0</accession>
    <name>A0A8S1T8A0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dynein light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0160311</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T8A0"/>
    <dbReference type="OrthoDB" id="10033309at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21450">
        <property type="entry name" value="DLC-like_DYNLL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001372">
        <property type="entry name" value="Dynein_light_chain_typ-1/2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01221">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01375">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8147304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="105" mass="12023" checksum="0220231B3A049E94" modified="2022-10-12" version="1">MDSDDEKQVALPPIRIRSSEMKQSLMKDILLKLDELFKKQTLDKDVCVELVKHLKSKPEYKIMGEGEWQCIIGSNFGCSLSYDLELLTFFDFLSNGKSVLLFKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T8N7</accession>
    <name>A0A8S1T8N7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Oxidation resistance protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0160154</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OXR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8146992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T8N7"/>
    <dbReference type="OrthoDB" id="289228at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF62">
        <property type="entry name" value="MUSTARD, ISOFORM V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="5">
        <location>
            <begin position="221"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8146992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="456" mass="53772" checksum="53D0C46BB2B4CAB0" modified="2022-10-12" version="1">MGNCYDSRKNTVILAFDQTEYTQLNQQFQYYCELSSETRDIKQLNQRSFEDIFSENPTFGIKLFKFLELYSGSDGFVKKEPLFEFLELLVKDVQTSVPAFKNLERFELMSLITLQNYRYVKSKEELAQLQLTYLDTLSVIKDIIKMYQNNQKNTPTNEKYIKSLVDQVYKNESGSFSWIQLMDFISKQMPGTKYLIKKYFQAKFMGKQFNNTIPVVNTPSYFVTDELFFQLLLSTQSVLTDCNQLTLLYSSVAHQGGFNQMVQSLKDCKLPTIMFFQHEEKYEQKIKQQIFGALTNLRWYDTKQYFGTKKDCIFSLYPYFKIYRSKRKGEQNYCFLDSNKGIGFGGKNGEGCRIWIDKNLQNSYCTQTDDSYENGPLILPYVKKLQINIIEIWAIQHPTDENDENFDIKIDLKDPLLQQQSDEVQYPNSNADFYWVSQQKTEQRNSGFYWENNNVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TAK6</accession>
    <name>A0A8S1TAK6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">RCK N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPENT_87.1.T0190125</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TAK6"/>
    <dbReference type="OrthoDB" id="10035564at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005267">
        <property type="term" value="F:potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000146">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000797">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003929">
        <property type="entry name" value="K_chnl_BK_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047871">
        <property type="entry name" value="K_chnl_Slo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048735">
        <property type="entry name" value="Slowpoke-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027:SF10">
        <property type="entry name" value="SLOWPOKE 2, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03493">
        <property type="entry name" value="BK_channel_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22614">
        <property type="entry name" value="Slo-like_RCK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21014">
        <property type="entry name" value="Slowpoke_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="208"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="232"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="14">
        <location>
            <begin position="630"/>
            <end position="771"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8149470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="994" mass="114137" checksum="E779290E2C04286F" modified="2022-10-12" version="1">MIYEGKQSIFSHQDQYALIRLVNSEDDATTKKHEEGKTTFTLKMAKYLSTSGIGDYLDGINVLLSFLLTVLHLIDCSFWVQGGTYDVGEAQTFITIPELICYIYFLLDFAINFYLSENKLFFTFQTTSLIEYVSIFPSLLARLNIITGYKYIYLLRVLRFLLCYKLDKVLQRLSMEVFRLAYKPIVTVMAIIMINSSVLYVVEQDYSIVEYIYFMVVTISTVGFGDVYPTTIYGRFSIIIAILIMFLVLPTQVEMLTRVYSLRSQYARNKYISKKESEHVLLLGSSQVEGFKTFLNELYHSDHGMNDTNTVILQPQAPTEEMTLQLKQPALQSRVIYLEGHPLQNKDLERCSSKDCNCVIVLANKTSRTPKRDDYRNIIHAFAVKQFAKKQKSRKGARVCLQVLQPSSKDLYFNSLGGHETDQVICVDELKLYLLGKTCLCPGINTLISFLIQSSKPSYDITKYDKNKGEWIDDYLCGMQNEIYRVPLESEAFVGLTFSQISQAIYKELNIILFALEVELESGTSVFVNPAEYLFEDYLHYGYVIANEMPNIDQIQQTKFPEYIQKNYCFPNVQRQQNNKNAIQQEAEYLKEILSEGIKDDSSKHPAYYQVKPQTITTGLPKVGDQEKFENHFIVCGVVSDMKYLMMPLRARSLKNIQPIVILNQDLIPTEIQLQINKFPKVYFQQGSPLNTEDLKKACIQKASALVILQKSADQEEGLSNIVDADTIFIYKTVKLLNQNINIITELASISTISFLQISRNNYVQKYDWSVSEPFASGEIYISTMLDTLICQAYYNPFITSIFDQMILGSATVNKKNKKIYQASKLQQSNLFLINIPPKYQEKTFGELFEILLTEQKMIPIGLYRGEKVKNNNKPYVFLKPPMDVIVSSKDRVYVLSAKQPKEQETTLDNQINQGKESASQYFNIKSKLQGEEGRVDIEFSRELMKFNEKMKNFGTGLKQINSSVFNQGLIQEDFIPKIRSLLKQELASLNPQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TB15</accession>
    <name>A0A8S1TB15_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0180066</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TB15"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8148439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="78" mass="9226" checksum="B29F0D8CA247274E" modified="2022-10-12" version="1">MNKQDITEFLNKQLILKYQRNLIEGLIQFIISQTKLIFQFLQNICLIKNTCLKKVDPGFLNQSIIFRINISLIFKIPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TBJ6</accession>
    <name>A0A8S1TBJ6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0180342</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TBJ6"/>
    <dbReference type="OrthoDB" id="310552at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8148987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="108" mass="12993" checksum="52A4D7D06EC87BF0" modified="2022-10-12" version="1">MPKDARGKNRKWGNHKDRQFESVNEGELIEQLKHQAEDDQEDDEEVEQQQQEQPQKVEQKEEGQEEQQQEKKKKKKKRVTAEGVPKPKKQKRNLDMPESEEEDESQYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TGY6</accession>
    <name>A0A8S1TGY6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tubulin-tyrosine ligase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0200280</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TGY6"/>
    <dbReference type="OrthoDB" id="302381at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_125, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069">
        <property type="entry name" value="TUBULIN TYROSINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8150476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="667" mass="80128" checksum="2A31DC32ED1A8E46" modified="2022-10-12" version="1">MFQSSLSHKKLKQIDIRKNHHYYLNAPYQISILSPEQQKFEQQNESANSQSTYQNNSYTQYTEDLYFRKHSKLFRDLRSTSNQSNNNQLNDINQTFIFRIKSKNHIQEEPLILDNLGQREKDKKTKDLVKVLHEMNKRRQKYIQKEQFIPRSPIPLLKRQNCIYLDEPQVEKLSNQIKKLPMIKEQSVLNNTQKDQKDIQSNCQKLSGHGNLEEQFQSKLNCLQQFIFQLQCNYLIYVKQSQVRQQTYFIEEINLNEQIITQLMKKRWWWCESPEKSDDVQFYWSLKLNSQILQKQKTHILFDITKKRVVNPIIINNHEEFLKFSQESKLTIIQQNCRIHNHIDLYQIFNLKKNLIKCIYTFQTLTNEELQQYFPYTINVANLQDPQFNIYQIKYKLTNSLWLVKPGEQNNNNNQIKICQNTKQVSDFIISELKNSNRRSDTFIIQQYIKPFLYQKRKFDIYAYILITSINGIVRVYFYDEVIGRTSSKEYSDNDLDPLTHFTNSQIQKESKSFSLYESGNKLSFYKLQEYFNQFKINFKEIIIPKLKMIATNLFKSIFTQMILRDFNFEIIGLNFILDTALKPWLIDTNLNPSFIPDCPYHQKFLTQLLDSVFQHTLDLLYPPPLNWPLNKKQLIENFNIQDRFSVIFDSRFDSEILLELYQKEIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TH04</accession>
    <name>A0A8S1TH04_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">mitogen-activated protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0210138</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TH04"/>
    <dbReference type="OrthoDB" id="297447at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06926">
        <property type="entry name" value="RNAP_II_RPB11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07852">
        <property type="entry name" value="STKc_MAPK15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000238">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000166">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.10:FF:000041">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00261">
        <property type="entry name" value="RNApol_arch_Rpo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037685">
        <property type="entry name" value="RBP11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009025">
        <property type="entry name" value="RBP11-like_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022905">
        <property type="entry name" value="Rpo11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13656">
        <property type="entry name" value="RNA_pol_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="133"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8151033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="679" mass="79491" checksum="DCD62F599175BFA9" modified="2022-10-12" version="1">MNGEDFRSKLREFHLRSKEQSTQDSRVILEDEKKMNNACCVKLYLEDHTIGNLTRMNLLKQKEVTFSGYRQIHPLQHTIEIKVQTNGSIKPYDAIQKTLSDLIDEFKNLEEEWAKKNVIQDGQSVDENILKLYQLLEFKGKGAYGVIWKAMDRQTKKIVALKKVFDAFSNDTDAQRTYREVIYLQQLTQHENIVKLLHIHRAINMKDLYMTFEYVESDLHKVIRANLLEGQHVIYILYQILKCLKYIHSGGLIHRDLKPSNILIDSECKIKLADFGLARLATDIDEFTVMTDYVATRWYRSPEILLGSPIYNNSVDMWSVGCILGEMVLCKSIFTGQSTLNQLEKIVEVLGKPSQEDLIQINAPIADKIFREIQMPRRKSLFTYIKSSDTIIDFISKCLTWNPNKRMTVDQALQHPLLNDFRGTEKENNYPSKITIELSENIKLDKKKYREILYKMDVNYQKQQYIKQLSNEEQIPSSIKNKKLSNQIQQTYQKYLNNGIDTKFQRVSKSSSKQNQLHQNYQSQMDIQSQILLTQSIQQQNQQIMKQKMLQNRIKTLPENYVDVLIPTEPKSPNRERYFSQNKITAQPQIPQSSTPIRSKSMKNRLYNESADNCVQIKENESKKKLLTAYLNQKPKRPILYNFKKTIIRNKLNMDQYLIKKHHGQLNNMDTSSMVSIAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TKR5</accession>
    <name>A0A8S1TKR5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0230287</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TKR5"/>
    <dbReference type="OrthoDB" id="310405at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38019">
        <property type="entry name" value="KDA ANTIGEN P200, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38019:SF1">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="150"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="264"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="378"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="544"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="612"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="459"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="685"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="378"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="544"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="614"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8152880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="733" mass="89158" checksum="FE3C94E12D87834A" modified="2022-10-12" version="1">MKVSLQDFGFHQPNAPEVNTPRSLEACRREGISPGEIVKVSFEEYQKKYRLSNLDAKGIETYYKHFEEKREEKYRDLIKQRHIVLEDEKTGLWSPDGLGKSKMISKYSQQDASSLIEKERQQIEKIKKKQQKEIENMLEFELKLQEIKEQNEKKMQDERTKQKQRELELERKRAQQEEIKRQKELQKQQKKEQEEQALKERMKLLESKEQERIKQEELKQRLREEENKKKEEEKKAQQEKLRKLNEENIRLQLEALEKRKIEMDEKAEQRKKMMEEQKEKKKQEAEKARIENEERIKQAKERNEQEVLRVKEDVEKKLQISEQKRLQFEEEKRKKLEQQKIEAEQHAEIIRKVIEQNEQMELEKKKEYLKKIDEAEERRKQLEKEQEKEKEKKRQEEHEKEQQRHQVLLQNEQRQKERIEDFLKKFQEKDDNLQKIQFERSIQITDKRNQDTLKRIDKRDNVERIMKKQEYDRMKLQEKLNEKMERADKIQEDLEQLLIQRQNMRREIDKQKRDLLLKLEKIREGKIPPSEIQKQFGITTTDLKQSTQEEQNFARKSQSQSINKNNKKLMTLTKPFSTIRSSKGQDKLPEINSSSPQLQETLNNHANTKSVESAQLLQQQPQQQQSQKKSPKRAKKQDIIKSKPAEPPSPEVLFELNQIIQRQNQEIMRVFMEEQANENRRDDKLRNCKSENRAKLEKLQSLERQKAQQRIQKLQEQHQYEQQQFKLAHNLID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TM26</accession>
    <name>A0A8S1TM26_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0260298</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8155071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TM26"/>
    <dbReference type="OrthoDB" id="5673at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011419">
        <property type="entry name" value="ATP12_ATP_synth-F1-assembly"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21013:SF10">
        <property type="entry name" value="ATP SYNTHASE MITOCHONDRIAL F1 COMPLEX ASSEMBLY FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21013">
        <property type="entry name" value="ATP SYNTHASE MITOCHONDRIAL F1 COMPLEX ASSEMBLY FACTOR 2/ATP12 PROTEIN, MITOCHONDRIAL PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07542">
        <property type="entry name" value="ATP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8155071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="266" mass="31096" checksum="FC30F14F79BF208B" modified="2022-10-12" version="1">MKGLLLKFKFTEQIQISQTLQIKRFYKDAKIEMAIGIANPKNHWLIKLDGKAVKTPFKNVLTIPTPQLAQCIADEFNSQKEMIKPNTMPLMTLARNAVDIEADERMRIFVEQSIINYLERDTVLFRDQSQSELYQIQKQKLDPQLKLFNQIFGMHLKVNFGIDVEPLKQYDQIRIKTILKEMNSWQLVGLTSKVENLKSAILALQIWNNQIDIQEAIKLSRIEEDYQISKYGQVEGHHDFDENTIYYQLKAAKLFAQLIQIQSITY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TS99</accession>
    <name>A0A8S1TS99_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0250045</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8153816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TS99"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000592">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF206">
        <property type="entry name" value="HSF-TYPE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="3">
        <location>
            <begin position="9"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8153816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="282" mass="33755" checksum="1ECDB4B5896E03A2" modified="2022-10-12" version="1">MTTQSGPFSTPAFIIKLYDILDEQVQFNQIIKWSDDGEYFIVLQPKLMENEILLQYFKHNHYQSFLRQLNMYEFIKARNSENYEIFSHPFFKRGNKKQLSLIKRNPIKTKCKVKKELKSAVKQYDFQKQMDSELLFLKQRQTKFETQFQEIISQNEQIMDQHKQIWQELSNSKKTLDVKIDRISYLLSFIIQQQGINNQDSIEMLNQIQCKPKQEYQPVESLSPLLKMMQNQMTWNLKVPTQLSTYSPVYNDQAYSPLAIRSQESLDSYQSISPFYQQFKDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TTK6</accession>
    <name>A0A8S1TTK6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0270071</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TTK6"/>
    <dbReference type="OrthoDB" id="295691at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8155398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="293" mass="34302" checksum="782878B4CB4493A5" modified="2022-10-12" version="1">MLEEQTREIRRRRIITQEQMLNNKNTDKQRRSNRFDNQKNRDGSPAQSNSSGSQNKTKFSTYRKATNKSPNSIERYLMSQECNIPTDHSQQDNNKKHKIRDQTFKIGQIYFKQENQDLQIQEVIEKLHNTSDIKIESPHFQNSKKNLENKQSLDQIQSIKDPSASMKTPTTTALEVSHNQTMSNPLLNQHPSISRDSSPTKISHPITQVSIGPISNKQSLSVITDQQQLKNRSRTADLFDDQKSVTIRSVLKKKQKNNDSFRTQQQESRKVRFDLPNSHYIRERQRQQPRLYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TUI7</accession>
    <name>A0A8S1TUI7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">MIR domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0260366</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8155207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="295684at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006816">
        <property type="term" value="P:calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014821">
        <property type="entry name" value="Ins145_P3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015925">
        <property type="entry name" value="Ryanodine_IP3_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45816">
        <property type="entry name" value="MIR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45816:SF4">
        <property type="entry name" value="RYR_IP3R HOMOLOGY ASSOCIATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08709">
        <property type="entry name" value="Ins145_P3_rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="2300"/>
            <end position="2316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="2341"/>
            <end position="2362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="2481"/>
            <end position="2503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="2557"/>
            <end position="2580"/>
        </location>
    </feature>
    <feature type="domain" description="Inositol 1,4,5-trisphosphate/ryanodine receptor" evidence="7">
        <location>
            <begin position="85"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="6">
        <location>
            <begin position="2442"/>
            <end position="2590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8155207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2682" mass="318900" checksum="FC9FBC0C1902863E" modified="2022-10-12" version="1">MDQDESWGSLQQIIHEDENQNNVRQGDIIYLKSYLPDDNKGLISGEGFATNKLECLLLQKYSNEYFNSKEFVTNKQTDSLVNKQAEWNIFKCLFRILDSNRSIYEQTKNEFVSDQLKENEEQNESEKNIIYGQEIQLLHLYSKCFLTLNSQVLAKENCCRELRLEQSITSNSNFMLQSNTKSYGEPVLYGDILYLKSSVIHLNKWGLGIQQTSPYSEVHASQIASTLRICNYLENENENIIMNGDIIVFKNRLFGGYLSIKRQFSSTKLMEKRQIYKKDQLLEQNYINLIQLKQQENLDKMFHITLESELQLNSLWQLIGIASQSTQQQNNKKDRNRVQKSYLIKNVLTGTFLYLENEQLHASFDRLQPKFEFQIRNLQKTEQQINLGEFYRILRIQENPQLSQFQTFVIQCNNSNEVNITTNCQKTQFDQQLFKVARGPYQDAMAANKIQSFYFGIVDFYIFLQDWALDKDLKYQYNLAFQNQKQLQDEINQFEKLLYNMNVFLSQENSIQEIKQCQFNLCQYDVLDLLIKLQQLVNLISNQENKWASSYSDKTAQRIARQKFDPVASIKKKKQFNLVEEIYQTIYSIIFDNVDCCNYVIQEDSIIDFLFSQLNQFREQIEKMFKQIVSKAKYNPIIAKKWINRIQMIKEENLEDQMFIITIINLFISSSYHISVECQNLCRKNLFINNKCKLLKCLIIEELPIIQIEADAYEFQQNNQKFCEDYHKYYLNLRQNQIAIEYFIKHDQERLYRKYEQYLLNLLNLYAKMCRGRNFKNMNLIKNVAGINYQFFDSIIKSIHSLQCKQVIIELFSSLYFDIDPLTKISVFENTCYLRQALGDFDKELESGRYFYENISSNILKKTDSYQNFQQDTLRTQENMQELLLLKSFSSHIITNQKYHEYFINLFLQQEYPTILTEDSNSSLKYKEISKIQLKFFLSVIRIIKDSIDLGYNTLNKNREIQETLPNIFFILIIQLNNKFKTFEFQEDKAFILNLKNMQQAQSKQISSLFERFNLRFNQSWIADFLLWSTSTCNDQDLIEQIFQEALNIYQVLFYLQANLQVLQYLTQKESSMNELFANHDSILFHCLFGATKLPNLNNEILQYLILSFNNQQYDLQEIKEVEILDDEIERKYFGIVNGHYEAQGDQGPKEIQQYVKQAVKFVQIDQQNNKKLDEFEKSFLKISQYIQKINSLFESIRIYNQSYTKSLQNMIRNAGIHLIFIEFFINSDQIIENEQVVYFYRNFLKFWENFLKDNQTNFQILIPYLDQLLKIILLPQPQHDNFLYKSLQISKLQLDSASNIIIDIIMNICKSLNLNEKVIHIRAAKQYLKILKEFTKTPSKPKFNHLSNQKQILHLILQNFRNLIDPLQYFEDKNLKDKQIQSNYLRYYQLKLHSTALNLISETCQNYYLGISETQRILLFEQLTSVLQNEPYIVKAAYLKCIFELYINFVKSNNLIGDLIDNYQLKELFTSIIIPDLQKMQQFQKSHNLENLILLNNKEYWKYILYVFEFLISVIDEFRVPERQNDINFVEEYTKINTLIKGILLNSSSPLIQEKREYIQQISEIIDLFVKNLYDFNLQNRLQIYCIRQKISVQFLIDYVEQMNQKDRIYKLKEICNQNNKIYIKQLDLSLIRSNKASHYQNAENWIDFKKAFNSQIDLNETTTQAQERLKEELNLQVLKEKFQERKEEEIKKTFLKKGCQGEILNPLSEDVQILFFLHELSQQVDSLLQEEKFKEFIIKCKQIFSQHPHYLIKLCKIFLEMKKPVKNYINQGELDEEDDINERGNQKDYQNTIEKFTTFQNIISECDIHVIAIEQLNHQNTIEQLDCLTLIHLLLDFGNSQVQQRFYTLLQNHKKHKKIFIQFFKDFYSTDVGIKLEELSLSKNTNYLHICIKVLQVLQGLCENVHIEFQKFLRIQNQQDSITNNFNFVLETTNLLVNFLSDKSVDNLKKWELYQQGLLSLIEFSTGLNENKKEITKNSRLFSGFNELFNHVDLDKALVPQSKKIQNIYFQLLQTLYIYIQLLISLLEGNANENKQVYKFLLENLNNEFLMSLAKKIYNIRINPKKSAICVDKYCRLPCKDNLCNKSFCINGYKTREDSLLIETGLNIFIIGLKLSNYSNDQKFKIFQFESMTIEKDLIQEEQIYQNISLRNVIEKTFSISQHPEYYQRVVDTQQVNNELFDEISEERQHTEAKLVQVSREDEFFLFYREFIGRIEVVNQFGQIERILFQKPFVCKYMTQNIKQNLIYESNRETDEDRITSFLENINFYHSQMAHYQNVSTIPIMHFGQKHWRTLKDISYILCMVIVLFFILLPDDIADGNLVQGDNDEDEIITDSKKVQIIITVIQLVLNLIIIIFCAIERYPIAVNFRRGETNVKQIQNLKIEAGFKIPEYKKKYYEFVGYMENNFEKQQVKKNPFRKIIVLLLIDFDNFYNLIIFGITAVAFFNNYVYAILLLDIVKRSEVLQNIIKAFTENTKNLLIFGLLGLIGLVLYGFLIKLSFSEDFKDDTKFASDSLGYSIAHVINFGLRNGGGIGDTLSDYADPFEDPAKYWKRYFFDITFFIIFNILFLQMIFGIIVDKFGELRDERQELLKDIQGKCFICSLDRNEIDSKTKNGWYQHIYLEHNVYHMLFYLIYIKKKPITECNQLEKYIKDQMENRLSEFLPVKMTYELQNQQKQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TUT3</accession>
    <name>A0A8S1TUT3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0270007</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TUT3"/>
    <dbReference type="OrthoDB" id="538223at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="33"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8155287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="252" mass="29772" checksum="8DCB355EBFDA03E9" modified="2022-10-12" version="1">MNKSNQFISASHDKQIIIWSINHNNQWISSQKLNGHSDWIFCLVLNKNEDLIISGSDDKTIKFWQKKQQWLCSQTITDHTDIVCGLSLNEQQNKLISCGYDKFILIIEQSSQDYKWNVIQKITVEQYGYRLCFINDNVFTFQPYSKELMHVYEMSNTNKQYSKTKDILVKSDYNGCEVYFPQQYINSKCLLVNKNGYNVNLIRKNQNDDFITLQSIQFGTNFLFGQMSEDGEYLITWDDKSNEIQIRKCDYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U2J9</accession>
    <name>A0A8S1U2J9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0310245</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U2J9"/>
    <dbReference type="OrthoDB" id="298465at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="88"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8158252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="325" mass="38189" checksum="F277F055C5385576" modified="2022-10-12" version="1">MEFNCIIERESFQLNAKDRSLSISNKSIQYTFDLSLKVLITWLFRENEISGFLLENLVVEGDGLNMKKLKQFLSGKITFSGIQIFYFFHGEINVNKNNATKVCTLINRQTKLKFSCKCFKKSVLGIEKIHKEIEIIQKVSKHGFAPKIYECFESNNCIYLIMENLERIQVQEFMDDDIRVFLYNLIQTITSLHKENIVHKSIKMSHILFSEDNKLKLVGFGQANDTNIQNNEFRLDIFKIGMIMYKFYQKQNFQIDQLPVIPDEGNSLIQSLLDWKNDSPFQLNMLLNHSYFKLLKNEGIQLQIQCMSPKFKTKVYQNEDDESNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U670</accession>
    <name>A0A8S1U670_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0330015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U670"/>
    <dbReference type="OrthoDB" id="298777at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8159217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="557" mass="65036" checksum="5FBBE9DF50D173E2" modified="2022-10-12" version="1">MGNSCSNNKSFQNKKDKISQQGNQDSAEQQKTKNGQTENQNIIQEYECTYWHSNQHRWIQKNCKITLCRQKEIIYIIDGSIHRIEKFLDSLQRPEVFTNFEQIQYLQWYGNYGQNLKKVGKWIAYWKGKYLSQVGGYSEDGSKQGSWKEIMNQFCGKCQVFELGQYQNNQKSGLWIYFYGNKKIGGGFYNEQAQKKGQWIELSENFNDEAQVMYRGEYKNGQKLGIWDILDKNRNMQQFHIQFNNNFCVCVFSGGGLYDEGSDGVKLGLWVVILNGIKMVKKLVDGMFSFRMFKKINRCKTTLYIYNNKNNLWVFSGGGSYDEGGHEVKWGNWVEILDGFCCNCQVTHNGEYKNGKKIGKWDIWLLDSQENQKIGGGLYDEGGVGIKVGNWVELLDGNRCVAQVTQKGQYKNGKKVGEWDILYENQKISGGQYDEGCNGIKIGRWIDLDDEFYYNKQVIYKGEYDNGKKIGRWDIEYRNDSNSQFFKIGGGLFDERGNELKIGKWIDLEEGFYYFKLITCIGKYDNGKKIVTWVSMEQKWNKMNEGFKKINEWKYDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U6A0</accession>
    <name>A0A8S1U6A0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0340272</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8160310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U6A0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8160310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="192" mass="22901" checksum="CDCB27CABA360B15" modified="2022-10-12" version="1">MYFLGQQQILKIILLLLVIIKYNQFISLAYLRLKCLQILNREETLQLLLFEIINKTINHILIINPFPPYIINKKQNLQLKLFIQQMILLNITQWIMIILDQLNNAKYYLVKPINNKLTIQCAPLIVRRKDIEDQNIIKNRIQLHIVFVIQLFLIFGLKISTLSFFDNEIESDKINLSYIPNPFKNEKKHIEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U7G1</accession>
    <name>A0A8S1U7G1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0320045</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8158596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U7G1"/>
    <dbReference type="OrthoDB" id="305903at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:001281">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053238">
        <property type="entry name" value="RING-H2_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14155:SF627">
        <property type="entry name" value="OS06G0192800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14155">
        <property type="entry name" value="RING FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="326"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="409"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="508"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="508"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8158596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="533" mass="61788" checksum="7B9CDE60E1BFC5FE" modified="2022-10-12" version="1">MILLTILIGFSYALVMKSTHTTRGFNAIEIDLNNYDEFTLGQSTIVISLTYNPITSYPILSYCSITNLNIIKTIISSISQVKTQIESNKKQCIFDNNAILNYQSKQQIMLYDYNQEESEWIYNKFSIFGMKERKFSIYIFSSEETEYEITIANQEPLTCFSKSCNNIGFCSKSQPSFCICNENQFGTNCQISSRSYKGNGQETISLESQKETLISIPLNQSSINSLDININANQKIDCFLACQFEKDYLPFYDSQFYDLNKLDIGKITYSSEKIKECLNLFYEINKELSQQMTPKLLLLFYNKQATNVLINMSVDISNNDQGDDKIIYYILAGAFSSLILAFVLIGCALICRRRINKFEQYSTDRRNSTEKSQKEGKFHERQFSGDFIPVELYEQVIQEYPGLNVITECQICLVEFENQDLVKLTYCLHLFHQSCLDEWRKKLQICPVCRGDLTKQKYKERQKDQEIYQFGVIAGDDIQIDNKKIEEIIKREQRIKQLQFAQSTSVINTMKENSPSPSSGEPLRLKFQQKQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U9H5</accession>
    <name>A0A8S1U9H5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0370015</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8161775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U9H5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8161775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="434" mass="51175" checksum="D3D1122F400ACA09" modified="2022-10-12" version="1">MSQTTCCVTYGSDGICSEIHILLNHILQQQHIQTSSYYCDYCYYANDQSQQLTYAHFTTPTPSKFVMLLFNIEEIQQCQWSKNTQTSLLYQFSQIFKTNSLNCSTNTGFNYRWVPTLNQNSSQLEIMIIVMLIYNNFYSKIEQKFSCCKESLQFLFNIYQLNLQPILNYDGFANPQYFGPLRQQLQVSIINLAIKQFNKEFAVLALGQQAKIYFRYFHLEIFMVSILENVLIIQFIVQQYLQNFKSFIKVYLTTPNQFSVTSSLRIGGGTTTASQCENTYVNFDIANLVSFLNELLINKNNIQTNNFCKYTTFLFIQILQLNSYVKIQSFLIFVTTNQNNNICFLCSCKLLQFSQSTIMYLYLTYLQQLMLLYVMVNNWQLYSCQKYQKSCFNYLLFKYLKDCQDELPQFCFWFLCNMQFRGSESNLQISMLMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UCQ9</accession>
    <name>A0A8S1UCQ9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Calpain catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0360232</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UCQ9"/>
    <dbReference type="OrthoDB" id="268518at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022684">
        <property type="entry name" value="Calpain_cysteine_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183">
        <property type="entry name" value="CALPAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183:SF379">
        <property type="entry name" value="CALPAIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="928"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="952"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1016"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="domain" description="Calpain catalytic" evidence="8">
        <location>
            <begin position="417"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1127"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="122"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="139"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="247"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="277"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1129"/>
            <end position="1158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8161529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1158" mass="133011" checksum="C586E6EB119B2247" modified="2022-10-12" version="1">MYQTSQLINRRPNMRQLTGRARDIQDDDDDDPGISSFARSKVDDDDEKFKNQNSDPDFLDSLLRKKSRNDSKVDKSNNSSIIPSTPPPQSTSKKSDQEISNTDPKKGQTQTQNNSGNKDTPQQQQQQKQSQQTPAKPNTTQPFQTPDPKQQQPQQKTPAQQPSQTKTPQAQPQPSQQSQTPQQTKTPQQTKTPQQQPSQSLTPATNKTQQNQKQTPAPAPKEIVDPAKSISNKPSAKKEEQKNQQPLPQPEQPSKVQPPPKVEQPTKIEQPSKTDQSNKGNQKQPQNQQPQQNRIDQDNQQQEQGPQISENRPNFVRRGKNTQNVKDVAKNIKTLPEDRIKALEPKNNIEVSKKLSLKFAPQKEYTDPTFPPNDLQVFQVFGHGQVDFKRFNLGREQDTHQVVDPNDIVPGQQSSTLMQVLAAIAEQPQLITRILEDQVINEFGFYYARLCIGGVWKYILVDDLLGYFKGEPIGARTTIDNNKLKSLQKSNPGYQSEMWPFIIEKAFAKEYGSYEDAFKIATVADFLQETTGAPTTVEQISENVLKSLTQNDVALIHLADGIFTYLSQDGKDVTLRNPWGWVKNQPSTAQPEGEFKVPIKNIIGKQITIAKCVPNNYHISYNLKANNDAYSSFYVDVRQDTVATFRLHQRDERYFRNNISKKYDYCFARLLVFDEQQQLIAEDFGQFKTLNVNVNLQKGKYTIVILLDFLNEQLYDSTLTFYGNQSVDISRINYRQQPNLLAQLYTQVAISKGQKKQNGQIDEFTYVSDNKLTILAWKYNGTQPNKWSKDLSKQPNRNNMKPITFVNVADTQQLKVLTTEQIKQLKLSSWQNASSFNIEFTQQNNTYAVQPGLDSFLQTLWQSHLFGTKVILTFSIILGLIDLVTHFAFDIFSNSPSLTLPFQIQRLFFANFVHSIICIHNKLKDFEIKHGTVTFLIIIIILGIITQVYKQILFQSLCLILQFIFSYIYPPFYETAAYSLWNFTIFFKIQECLMIPNGQSQFLIFPMQLKNKYYPIVFVILFTLIQQSLTFISASIIAIIYFLFQNRFSFQTATIEQLEKGMIFQSFIDRIDFKRISNYQDQLEPSVDSRELPYLETPPARLTMDGSAPFPPSLVLQELQKQLNFQDNQNHEQVQEDEEQKQIEQKQSEKDQEFDQDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UE39</accession>
    <name>A0A8S1UE39_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0390080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="303797at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1007"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1221"/>
            <end position="1244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1334"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1585"/>
            <end position="1608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8163158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1646" mass="194950" checksum="9B7E7027F1C4F132" modified="2022-10-12" version="1">MKYFSKLVYFFLTIGSNTERRKQKVDYGNLAMIVFYFIQLLGYLYSQFESKTQVFYENDYLAQIGQYSSIPFMMIVIHYDPFTTLVYYCIFGTMCSIYGIIIFQILFKPLPPSNLINRFVKFYFQNFQWYFLTPFHETMIGVLTCGRLAYLEQHAGLNPRNCFAEISPHFLVISFLGQALVIISGFLSLYCFRNYEFIQGDLMRKFSYLNFLTILLHMILQMASFWKEMYDNYNIIIHVIFIMIMFIISLDTFSNFPFGFSSETIHFSQCLLSATFFEILVAIWIFSDLDDGHIFLTFCIVVPLIFGINQAFYDNYIDNQCQQFVRSRCKTISEHPLEVICQLCHQDIKQQHDYFLVLKYLEIHCQNCCDLICPCKQKINKFIHNHNQLSTNQIYLWVQYEFQQMIKSILQNTELFKQFEQLTIKFVTFLQKYRENQVLSYKIIQDVIQTFKKINSDKKPFFFLNLAKHIQYQNKVEMENINKAGSQISQSEYLTLQEFNLFYRYEQSLVSNLNGLLVTQKQLWIEKLRNQIQLEKILSCSNDIMKRTLILKKIIKKFENSKESPKIDTILTLRIKLVSSLVCMEDINLSMKIARHIQSYEKDLINQKQLNFNSLQFIQGQALSVISNISTQTFGQISQQINDQFYNFFGYENNNGATLTKIEQLLPNKIGKIHNGLIESFLQKGKSSRLYQNSDQFIINSDNLIEKVSICLTTLFPYQNEQYQFLMIGHILKRMKYEKREIDYNKRGYILVDQNFLVFGLTRNVYERINYKYFYKNEKDLELIMPQEIYDEYSIQTLIPQLSLILEEYYKLLALRNEKKILKHDIICQREIGVFQCPSTKIQNVKTSSTLLTKKNTNKHMMNILKDANFSLANIPTFSQKQYPIEYSVTQKVLDYVENDAINEFLYFIIELDFLEDPKLNATPSQVIARSPALSDLLRYQQIQVEQIRPNQSNIILSNDDNNDQIELMAELTNTGTRRSSNEIIDQLEKIIIYINDTLLPKSIKDLIAQFLIQILVFATIIIVLSSLFKHKRSIQSDCIEQITYDLNFLDAYSQAMAGSRHVIYYRDFYSIQNNSLIYLNLNQINFSRYDKIYVAWQHISKGNIRLNKMYENYTKRAQKSQIKLLTIYFVNFDLKSKIQQEVQDYSTYYQIMHQTFYLQYQSFSSSPQNYLSGNDDSYITQLARSQVYYNFFDVIESANFTLQDCQSYNLQINDYFDTIIIFYFLGMYIILILVLISIIIHYYRVVKSIKIYIKLFKMCDKEDCQHIIHLCESLISMVNYDKIFLRQQDFKVIMAQPRHTLENVKKSQSKTTIILIRKKSNNQRSIKNNQNNLSFYSIIMLILISIGLITYILSFHLYYIHIKNSITPISNQAILAQESRLNFIASVNRFDLYFIKLYYETYSRLNQVNENYQFTSKNIQTDTQILQLLTINIETLILDLKRLKAINFVDLIFHNDQANSQLDSNQQDQILKNDVCIFTGCNMQTELLFDRLFKNELMPLFYTGVLNLHTKILSFIQDAFQVVQQDLSDVEIIMNLEHIFNDSDYLIYMHWGLDIIQFQIQEFSQYFLNISQQTINSLTQNNQLLIISIGTLLLILISIFSIMFINIQYQRYVQSKAIIKCIPLPILFQKNIPKHLENFRRKYEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UEY7</accession>
    <name>A0A8S1UEY7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0390186</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UEY7"/>
    <dbReference type="OrthoDB" id="301113at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8163370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="53" mass="6276" checksum="93DDF50056B34B8E" modified="2022-10-12" version="1">MDNTETTVRNGNSEIKFKIIEWMGNNQDASNLLKIKKELAKRILREQKKPQQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UFJ1</accession>
    <name>A0A8S1UFJ1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0380120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UFJ1"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025663">
        <property type="entry name" value="AKAP_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14469">
        <property type="entry name" value="AKAP28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="176"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8162559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="471" mass="55872" checksum="92499F4E9A5243B0" modified="2022-10-12" version="1">MTDLLERSIHLINNFVSQWTKKYLYYTSYIEETTSGHTFMTVFSLPTPSNPIPVATVKVYFYIPDLTDPSEKTMSFRFENDSLIHLVNRTIRISQMEKWIETILARKDRTCRIMFLGTEFEQTRIINKRMDEVLYEKQPEQEKENLVIQPELLEHDQELVNQFTITEEEKIREIKMLTELLYQCFRQIDKDDMGVISYEEGGQLFLLMGLHLGKHQLEDTLNRLDVSRTGILQFKDIQSFGIEILHSIYCQNQALKELREKEEESRFEAQWMLSTDLRNVYKKIVEQCKLKDEDEIHSVNSNVFQQILEEQQFFTEDEIKQIMEIIGQKDVEYQTSDKVLEETIFENLVKGLLEAQRSKMEVFLLEHFKRQDKDNTGLIKINELMIALKNCEKIKLSKVQLYSLQSYIKRNDKDLVNYKQEARKIGAIIKKFFNVDLMEQRSRLPQHTKIDAYIPLITPEQIQEQIKKVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UHA5</accession>
    <name>A0A8S1UHA5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0410090</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UHA5"/>
    <dbReference type="OrthoDB" id="1711136at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047126">
        <property type="entry name" value="RNF141-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109">
        <property type="entry name" value="RING FINGER PROTEIN 141-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109:SF5">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="2">
        <location>
            <begin position="95"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8164551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="165" mass="18755" checksum="980477E9F848B8C3" modified="2022-10-12" version="1">MIKLSATYFKKKELISIKNSPIGSISNQDKKFRSISFSEIKQNKDNKKQSSQIYDEELTKRFDHLLSSPRLKLEVSESIGAELSSRVQPKQIQLCLICYEKESNMINQPCGHGGFCQECSQQLLAKSDLCLLCRKPVTHALIIQGVENRESLVEVVGFFQDAKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UHC9</accession>
    <name>A0A8S1UHC9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0370188</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8162076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UHC9"/>
    <dbReference type="OrthoDB" id="287764at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035914919" evidence="1">
        <location>
            <begin position="21"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8162076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="171" mass="19664" checksum="3233EC25149A93ED" modified="2022-10-12" version="1">MKSQIILTLLFISALATVERDSNQEALEGVWSYFNQKGPFTVTKCGTDQDHKDAFLFTGYFLRKADIAQTSEINPLIAEGQQFYNSLPLETRNCFENNQEVIDLGKFLGIDKYTEDDLQNKVSNWVGTHYFVYKRLIKKAGSDWNTWEDYEQTGRDLAELLQGILTDAQEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UJ66</accession>
    <name>A0A8S1UJ66_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0410072</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UJ66"/>
    <dbReference type="OrthoDB" id="307032at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8164515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="60" mass="7160" checksum="31266A2B095CA39C" modified="2022-10-12" version="1">MNKLIEKKESKSLDLYVVLRNLEYLKDHLVISEKKINRKKIKIDDDLEKTILICNSDQED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UKL1</accession>
    <name>A0A8S1UKL1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ras-related protein Rab-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Small GTP-binding protein rab1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0430066</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Protein transport. Probably involved in vesicular traffic from ER to Golgi.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8165668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UKL1"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001018">
        <property type="entry name" value="Rab family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="176"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="201"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8165668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="210" mass="23953" checksum="A570D1C40DE79D29" modified="2022-10-12" version="1">MSNSYYDWLVKVIVLGDSGVGKTNILTQYCDQKFSQNYMATIGVDFKIKTITVEDKKIKLQIWDTAGQERFRNITQTYYKGAFGIIFVYSIVDRNSFNNVETWIKSITENTTDEVCSILVGNKMDSQDRRVQSSEGQALATKYKMPFIETSALENKNIQNIFDILGLNLKKRLENESQKPQVNAEKQSTSQFKLNQETAQPQPPPSSCNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1URB4</accession>
    <name>A0A8S1URB4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PSI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0480035</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000048">
        <property type="protein sequence ID" value="CAD8167848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="296837at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002895">
        <property type="entry name" value="Paramecium_SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00639">
        <property type="entry name" value="PSA"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035803193" description="PSI domain-containing protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="1773"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="27"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="310"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="558"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="659"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="1284"/>
            <end position="1327"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="1655"/>
            <end position="1696"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8167848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1773" mass="195736" checksum="18FEF10D3CA4C0DB" modified="2022-10-12" version="1">MKSYILFAILIGFGATQIVVKTQTICGCTQLTNQNDCATLPGCIWLEGTNQCQQNNCSLLQQSDCLKASFYCQWNPNLNPPSCSVFISCVNLTANGNQQCIQQNIRCLGFNTTSNQCLYYSEITSNCIDFPQDICSYNFGKDGPCYWINDQCQVINQCSYAQQKDQCQQLNLFNQDSPQGIVCQWNETQNKCTVITNCGQFTSQDTCKYYFSSLNSIILNVCVWSQNTCVPITNLNNLTSINCLSNTGLFFRWYGLQSNLNSGFCGPCKQISLAPKAQCTCSDYVLQSDCNSQNSLCQWNIQTKICTLNTCSQITTQNICITVANCYWSFNTNTCQTLSSCDDLIFKVSSVGCAAQSLSCAGYEGGNCINTSTISSTCSSQQQQINCSQFISSQGLCVWNNQNYSCSLLQYCNQITDATICGNWLNQCMWDAKISQCQQLTCSTITNEQSCTYVLSQFQSTQYQLCRWNNSLGPQGACQNAYSALLQTSQTCLNNTGNTFRWSTNNESAGMCVSCGTIQLSLQTTSSCQCSQLQSKENCQNTGFCTYNNLTNICSSSPCTQYENQITCASLSTCYWNSTSGCQAFTNCSSLTATNQLECINMNASCKGISYGNCQSFPTQTCATMYTTSGNCYNNIGTDGVCFLSINEQTTTQCIGFSQCTQATNETLCLRNQLSCSWNNLNNMCSQINCSSFKNQLNCKFYLPNPLSSEVIPCVWSNNICGQASDILKQLTQNTCASSTHNTYSWITLSTTSGYCIRCQQLVTLPKQCICSFLSLYDCSQALECYWNNGSCLQLQCQNIIQQTICASQIGCYWNNNQCNLFHGQCYTLSGKNQTECMQQNVYCVGSNGINCIENLNNCEANTQDSSCLTSLGRDGACYWNKSTNKCMAVNSCYQLPESACKIRSKSCYWDATQCQTLTCSTLYAQFNQCTFVMKLSSQNYVQVCQLQNNECTQITNVLSLSQDQCYLNTNKTARWIPQSSNYGGICYSCSANMIPTYNFPTCQCYQYMTQSDCNNAINQSCIWINNICTQQPCSQISSNQVCAQTIGCGWNIITSVCENFTSCNSISGVGLSIQLCLTYSTQCGGYDGANCTSSPTITCGSMSPSTCSGTYGSDGICIWNPYITDSCIAVTTCSQIIIQQICSYYSDYCYYANNQCQQLTCANFTTPASCKFVMSLLNIGEIQLCQWSTNTQNPSCINLVTSSELNSVNCSANTGFTYRWVPTINQNSSTLSKNGYCTKCLMNIINIPGQCACNQLIYQYDCLSNPQCSWSTAITTPSCYNKPCNQIIQQGICSSNPRCSWSATQNICQPFSSCSDLYGVNSGECSSYSIYCAAISKTFLQFQQKYICAPTPNQCSVTPSQGTVGGITTASDCQNTYVNFGICQYNPNNQQCTKITMCSDISTQQQCQQFNHACFWQPPMKTTATASCVAASCNNFTNQQQCTYILNSLSATNVTLCQWQTIGVCIESTNTKNLSSTNCYSNTLMMSRWSSTQPNSGFCATCSSYSQSQTYKSPCFCNELSQQECLLASPQCSYNSITSACVVQNCQSIISQYSCAANPNCIYIGSCQNYIKSPTSTTYGCANITTASSPFDCLTASVNCPKFVPDTISGSLGKCYVAEECSKLLNGAVCNNYYNQAVAGYCVWYDNQCQTINNCKQIIDIEVCSLQSKRCQWSVLLNSCITQSCNSYTSQSDCNYVYTSYTLGDIALCYWDSFYNECRSANLSLAKTFNTTNSTQCYVNTGHVYHLNGNDCIRCFQDILSIFLITMFLILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1USM1</accession>
    <name>A0A8S1USM1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0470144</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000047">
        <property type="protein sequence ID" value="CAD8167484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1USM1"/>
    <dbReference type="OrthoDB" id="291626at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001087">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="28"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="343"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="419"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="346"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="419"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8167484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="500" mass="58547" checksum="846D6B03FF06144E" modified="2022-10-12" version="1">MRSTKKHATTQGENQFKLSVGQIIAQKFKLLDKVGQGSFGMIYKTENLETGDIFATKFEKREENSNGMSLLVREIKVLIEVQEFEGFPQIVFYGRDEHYNYFMQTYLGQNLEHLLKRNNYKLSLTTVCRIGMNLIDRLKLLHSKNLIHRDLKPDNMCIGYEDVDQIYLIDFGLAKYYRDQNGNHIPQTEKKGIIGTARYASLTAHLAKEQSRKDDIESLGYVLVYLAKGKLPWMNLNTTTKSEKYQKIKEFKQQLTLEKLCEGLPKCFLNLFIYARGLDFQGEPDYAFLKELFQKQLQQELSSLQALNTLEYEWERFPEIKKRKTRLQINQLKQKMEKLVQQGVDSDLRKSSEDKGQEHKGSSFLNLPIKDQQQQLDSLSPDIKNKQSRNISQNISSFGTSQINNYQISQIDKFKRFPTERKEQRNNTVDTQKKERETSPNQKIFIKQTTTKTERKMAFFEIDNDYRDYDHIDQIASEEVKIVFSNIIPNLGRHKSTLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1USQ9</accession>
    <name>A0A8S1USQ9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0470159</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000047">
        <property type="protein sequence ID" value="CAD8167514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1USQ9"/>
    <dbReference type="OrthoDB" id="340346at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001327">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055231">
        <property type="entry name" value="2AA_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054573">
        <property type="entry name" value="PP2A/SF3B1-like_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051023">
        <property type="entry name" value="PP2A_Regulatory_Subunit_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648:SF4">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2 (FORMERLY 2A), REGULATORY SUBUNIT A, BETA ISOFORM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A 65 KDA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22646">
        <property type="entry name" value="PPP2R1A-like_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22956">
        <property type="entry name" value="VPS15-like_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Phosphatase PP2A regulatory subunit A/Splicing factor 3B subunit 1-like HEAT repeat" evidence="3">
        <location>
            <begin position="275"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="2">
        <location>
            <begin position="281"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="2">
        <location>
            <begin position="319"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatase 2A Regulatory Subunit A helical" evidence="4">
        <location>
            <begin position="356"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="2">
        <location>
            <begin position="358"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8167514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="574" mass="66197" checksum="DCB1C01FBAD05D68" modified="2022-10-12" version="1">MDEEDCTIFIEELKSDDPNLKLNAVSKIVSIAQILGPSRTCQELIPYLIDIIEEQDNEDEFLIKLAKELVNLKPFTGANVHLLNAPLEILSSMEEPLVRDKAVESLILLAEGMPNSFFENHFFQIVQQLGQWDNFPSRISAASLLPLTYKHVSLEKQNTLWDLFKQLCGDDTPMVRRVCAGVLSDLAKMKCQPQQLLQLWEALLKDPIDSVKIKAIEGSQYMLKLIDDEHDLETQLQGYFALADPKEKSWRVRYTVPECLESIIDIIVKLNKNKTILKNQAVPVFQQLLKDTEPEVRSMALISIYHLLKELPSSSKDLFLPLFQTLSTDTSQHVRMSLAEQICKISKQYTVQIVLQSFIPLITTLIKDDVVEIKIKLAHNLDQLSQAIGQDNSKKHLVPLIQTFASEKQWRYRLEMMSIIPKLLKVAGYDSFLELQEVYLEKGVLNHYQAIRDQAIDNLIQLSEIFGYDKIREFILKCINLQFEQPNYIYRVSAMHSIAKLKNTLSKDDLVNQFKEITQKSLNDKVPNVRLNIFKLFTTIQNKLDNKTQNEFKNKARILQQDQDIDVKYFAQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UTG0</accession>
    <name>A0A8S1UTG0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0480015</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000048">
        <property type="protein sequence ID" value="CAD8167825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UTG0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8167825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="184" mass="22329" checksum="EBD2F57A6839F13E" modified="2022-10-12" version="1">MIHYIQYSKSNLPKLGLKMMAKDKQLRGNNMFRLQQMQMTKLQISQSPPTPKPKKKKDYVIMIFDRLSSHYIRYLDQFVIIEYFNKRDKVQFKFIYQILVQNQSSEVRIYFSSNIIKFIDLQLIAMSRMLQGREQNDYKYFKLPSYLINLTNNEFSSYIKNHQYIIPNIYPISLKLWIRLMNQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UTR3</accession>
    <name>A0A8S1UTR3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0460168</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8166979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UTR3"/>
    <dbReference type="OrthoDB" id="276029at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8166979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="57" mass="6977" checksum="4089EBCC0D0DBBCC" modified="2022-10-12" version="1">MLQDFLLFKFQMLKQTIKKLLFIKLPNYQEFSITKDNQIVLQADKKVIYPIFYYLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UVN1</accession>
    <name>A0A8S1UVN1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0490001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UVN1"/>
    <dbReference type="OrthoDB" id="318383at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8168253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="203" mass="24501" checksum="354BC2D03F871F97" modified="2022-10-12" version="1">MDKYYQIQKQLIVIDAINFCLEDYYFYGVWSKYNPLSPISQVCNIGLFDNNCFNIHNIDQNWLVQISFIMMVLIIIRNKLKKTIKFSDNEKNHDKFVIQMDLLEHENILSQKSKQELNFLKCLPEKILRKFPLKFMVSYLLLKEIDSNRINISSRRKEIILLSNIFLEKIYNMKFDFHTKLKAISNGEFRFLQLQVKYKLFNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UYT6</accession>
    <name>A0A8S1UYT6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0520016</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000052">
        <property type="protein sequence ID" value="CAD8169871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UYT6"/>
    <dbReference type="OrthoDB" id="306942at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SPRY" evidence="2">
        <location>
            <begin position="158"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="77"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8169871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="276" mass="31776" checksum="02AEF7444EFA6B80" modified="2022-10-12" version="1">MELLVIESLNSEACDQYCQLNQNTYNSGLTQSKNSYTMIALLDIFSLNYLMISLQIVMCDMVIPKESLIETQCEIIVKEKEGELQMCQEQNEKNEEMLKQLQQTLVQQFSQSLTFSTIYKNSNCSVTQNGKLIETSQSGWQCCMCDQMIPKNGLIQFAIKIIELSSIMIGIGFRDIVQSRNYGVCHNIGYGTYNIQFQGQCYNHDQQDKHQKQIAFPFSTNDIIIVEVDIQKKYVKWTKQSTNQSFTLNIDTSKDLYPCVHFCGKCKVEILNQVFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V209</accession>
    <name>A0A8S1V209_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0530265</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V209"/>
    <dbReference type="OrthoDB" id="302796at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="131"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="378"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="749"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="258"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="449"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="521"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="784"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="160"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="393"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="749"/>
            <end position="766"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8170824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="875" mass="103946" checksum="FA7B9264DDF2A682" modified="2022-10-12" version="1">MLQINIEVQTVYFYIDSPIMYCHMQLNDSVTAVMLNSQLKCKCYEQQDIYEKAEIRTHYDPKNKKKLKLIFFENQSKFGEAVFDLDFYIDNKFQSLSDKLSIQSEQNEEAQLTFMFEWQFIEKPQEINPKILNPYLSGNSPQNTQRNGGQSPKRQVQSKRIGEENEKRTTHVTYSQWKDHKEYLKNQQERKLKEPKHSQKIIMVDDYNYNWTDNLRKSNAQVRVVSPPKRFGTPTKVNKKFQSPVNNLNPQRQQDYELRMINEKLNKVDKQLQQMNNENKEEDKKKYSSKLNQKLKQNQFDDLKNGSNEDQIISQSEKGIKQKSLHYQSFVEQKDSKDYQFMYNSQIQPPTQQYQNSDELVKSLHDPQISKQKIKQNVVNLDVPKSPPKPKELQQQLSQRSKTQQQEELLNSNQLNNSDKYDELMKKYLDLKDKYQNSCLEFSLLSQTYSSLKDEYTQVIQQNQQYQQQIVFLQQQQLINGNNEQGTQSNFNQEIIKKELEFLQRDNNMLKTHLEQSQYNVIQLQKEKEKNQIEIYNLKNNLLLINQEIDKLQNEALQMKTEIKQNNQIEQSLDAQNKINSKSDTQSINQMKTQLENQGMNFVHQKDELEQLKIQYYQIEKENTNFKKQIGNLNQMIQSLEDEITKLKDQLEEKKQDVIRIQALNNKDNIGDIIDGYKQVIKKKELEILDQEESIKQMKKQISILENELQSELDQKQQAEQKFNQKFNDLQEEILKLQTDNQNLKQILSQSQNEQQQSFQQSNKNSMHQQENNFKGFSFRGDDMDNQSENLKQENTSLKFEQNKDKIRIDSLTKELEIQKNIAELSEQQLMEAQEQIMSQKQRIADILNLIMIKGDAKQMDDVEKLLQNKEFLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V497</accession>
    <name>A0A8S1V497_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0560142</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000056">
        <property type="protein sequence ID" value="CAD8172190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V497"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8172190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="89" mass="10711" checksum="B37377FCC436FAA8" modified="2022-10-12" version="1">MSYRQRCQQQNNKIISIVLILESYAQIEVVQNKIFTIYIYFFSGFNLAESELIFYNDNNFQRRNIKFYGILNLVFKKKKSTLKNVELLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V4D1</accession>
    <name>A0A8S1V4D1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0550215</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V4D1"/>
    <dbReference type="OrthoDB" id="301165at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035765390" evidence="1">
        <location>
            <begin position="21"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8171791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="234" mass="26308" checksum="40F5DE52E8FBC8F7" modified="2022-10-12" version="1">MRTLILTVLICFGASQMLRGSPKALIEEEQAIFQAEQEDQSFYVGRDDVSAETLQGDQNQEVEASLAVFKSDDNPMVYIEESIDSTQQDGFQQQESLQIEKIEDQVQAEELQEATTDSANYVDKAISLEGDTEAQPVQNSSTDVVLNIAPKTESLSSSETTKKHVYVYNPLAKKPFVGIKFPPMPVTKDHNYLSNSDTLTIKLPPWSEQSNHKVEDDLEFFQFIRPSDVSVNEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VDK7</accession>
    <name>A0A8S1VDK7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0610158</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VDK7"/>
    <dbReference type="OrthoDB" id="5979581at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000616">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="18"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="327"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="376"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="385"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8174545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="472" mass="54392" checksum="435E5B9B8C3DF087" modified="2022-10-12" version="1">MMKLPQSYRDPKIFNNKYIIKQQISSGSFGIVYLAFDKHTREEVAVKVEKEENEDASSLDREIAILNRLIGVPGVPKLYWSGFEQDYNVIVIQLLGKDLSHYIKQYKKLSLKTVLQITHQLLITLQQVHEKGVIHRDFKPENILTGYQLDNGIIYLVDYGVSKVYIDNQGKHIPSKDKKSFIGTTRYASIAAHRGHELGRKDDVESMFYVMIYLLKGKLPWQNLQNIGHRDRTSLVGEVKMKTDIAELCKEIPSEFAEILNYLKKLEFKSEPDYKYMKSLILKAASNNQIIMDKVFEWSDRSTKIKDQIIWAVSEDKHKQSSQAILGSNHLLKPPEVNRQNGGSPVRAESKRMTSTSIQGSMSSMIIKYVPSQVENTSIKDEKRSRSKKSRKSKKSAKSSKRQSVGFHSGIQIIEPKDQYRRYKTIDSTWGFHQYMDEFSGDEDSQKLSTKYQQLQGMPVHFKNIHKPTSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VDU3</accession>
    <name>A0A8S1VDU3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0620122</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8175027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="306122at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="275"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="966"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1163"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1271"/>
            <end position="1293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1486"/>
            <end position="1505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8175027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1551" mass="181887" checksum="5CEE7BD5F51EC4BA" modified="2022-10-12" version="1">MIIQEFTRQPSKLIYFLAQFFNELLVLKCGLLIQKQDFKIESLFEKFLLYVIRPYTYFIHEELELLYSIWTFVLLQLIILFWVYLLYISDHKEKKTYIQLINKFESRSTLQFLLIYIWKFSFLILSEFLSLMLIEGCFVILFDHNIDNLFCLASSIIMLFEIMVILMLQLIFMNQSLTYIPRQLNQIIQPTLLVYLSKLLKIIVLCLFTSSITNKSIMVLVILILNGLLELYMLLIFSIKLDRYLVRQNVLMQCLIICISIQQIINVYIDQELQFLWIIVSILLYQSIYMYYVNDNILQTTEDQNNTTKIQSQLLQNQKKLYYYQQMTESRNSEYRLINKIFLSHHQKTCVKKMCYCNNEEMVMIKLESIIIKDLIKKLKLQIQQSQQQSSLFYIQYIQLLIQNGKCFEALNVTQKILNQFNRRTQQFKKSVQLIKGQISLSHKILFKLLQQQILISIKFSIQSKSTGSAKHQIIKQAIRSLQQKVDSEKILQEQFNLILKAKIEFLDEVSKKPNLEVNFINNQSIKIIKQIEDLKKRLVNNFKIVPTHNNQSLLLFFQIEILNDLINSQTINTMNTQLDDQEQIVNANNIYQEFASQYSYVTFILKEEEDIELVQDSTNRAIKIGSNKYTSFEEMVPSSLVSVHKYLMKDFFQTGQNKYYLNISQSFIQIQVGIISPIDMCLDISFDLQMNQLLSTAFIRLTTNNILIHYFLLDSKFKIREISQLLYQKILEYPINSKMIFFDQSIFNFIPTLTEKDFLHDRTLGLYQLQFPSNSNKSLTTHSKYSQKSIEIFQGVISVLVRKINQKNTYYIVEITDLKSDKKGVYDSITLSVQQQIDIDEKEIINIPFDICSQPEQSNLNQVVQQQLTSRLDSQRPLLFTSLAECLDVQIDNVKDSQSQISNKIENSVNSKKKKSKSEEVLKQFDDQGSQISSVAAVRRSAYFKQFQIVNQLIFSKIFPIHIKIFNLMYFLYILAGVLNLSILIQQVQKLQNYQDLLALLSIKYDIYEPIESFLNTRYTIVNLNQAVATRVINQSVFNELVIFPRSNLVKGYDDLKENIFDVISRKEFQTFLKDRYFDSIWYISSNKGSDKNITFRSGILALLNYQYDFKLAYTIKTLQTDGPYFYYSYKNYIPLYTIFTQLNSDILDQLTNSLSDQSDQLTMISAPFNVFLGIVSIIYYIYLIRQRRLKNKLLSLLYQQDNEIVAHEVQRLKQLQTQINNNYSSLYQYALDVQSFDDNLKKQEGEFFRLMNKKGNRTSGLRKNRQIKIYNFMQLGILLINIIVLQLLYYITISSLDSYLSKSKNTGQIYESLSNTGVDVLIIYAQREVLYRAALIPFFTPQDIDDLRVQINNSLESIQKFSGQLLQLNQNDYLLDDSSLELFDKVSSSSLCNYLPDVYSNVSTTLCPQVLSGVLSQGLISTLNVMYNSINSEKQLNNFTNRSNAQVPVRELEGATFTAKVIKELVQKLYQNLYNYTNEIRSNFTIIYICGIFIQLIFGFSILQKISAYEKEQMNDLRKVVYLLPQSTLLFDDSFQRQIKILIKQGQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VFQ1</accession>
    <name>A0A8S1VFQ1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0630057</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VFQ1"/>
    <dbReference type="OrthoDB" id="312622at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="92"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8175441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="431" mass="49567" checksum="D369370FFF23333C" modified="2022-10-12" version="1">MDVNLDIEIFDPSCNTTLVIDETVNILELIDYIQQTFQDKFQGVDTKTISIRIGGTNEIIQPTSRSLRDVLREQKKAKLFLQKGQMSSPSVQFQQSMSQRQFNQQQPTDQFANQTRNTPQVPQAQTYINQTQQQQNSPIGRTIQVIVKNGVQQIQVQLDDNVTIIDVIQHFQEHMHIDLKQYHLFYGQVNLSLQNQNMTLKQLGMSNTSQLEWKLISQINQRVSLPPNMVNQNIPLGQNFAGQTQYNPQNITQQTNAGQLQHQQSQQYQQLQFQQQQQQQQQQQQLLLLQQQQQLLYQQQQQQQQQQQQYVQQQNLGNQQKQGQIGQQLGNQQQTSSQQVQKTGKQININFEILDGSLKRQFSTIANTSISLSELLDAVYQYLGVSKDVASVDLYIGNQAFGGPKLDNSLEQLQLTKDNQTLQVKVRWIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VH87</accession>
    <name>A0A8S1VH87_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0640060</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8175883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VH87"/>
    <dbReference type="OrthoDB" id="418560at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013111">
        <property type="entry name" value="EGF_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051216">
        <property type="entry name" value="Teneurin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11219">
        <property type="entry name" value="TENEURIN AND N-ACETYLGLUCOSAMINE-1-PHOSPHODIESTER ALPHA-N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11219:SF69">
        <property type="entry name" value="TENEURIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07974">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035876155" description="EGF-like domain-containing protein" evidence="5">
        <location>
            <begin position="18"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="403"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="407"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8175883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="480" mass="55734" checksum="09A9CB6188CE8C2B" modified="2022-10-12" version="1">MIFLFIVKITIAWKTQSIKENIQTCQIFESGNQQECELNDLIIFRLDSYFAICLISSEIGVQSVLYENSQGNQNFICVLKDQILIYPLPYTCGSQFKRLQHQNQFQNYSLTRDNSSMYIFGGFDGSELRNQLFKYNFEDLFNNQITYSILPNNFIQKLTVSLDLWDGSMEDFQFTSNFPKLQKLKNLGYKYTVSSIQDNSQCQSSNIPKCLQYSVIVYVDECNCIKLFYGRDSNNINQNESWLYYLDDQVWQETTLDSIIKFGTTPIGQYFPNINMIGFYAENTYYFYYDWEWYKNYEQLNFGSMFKQMIAYDNKTFLLNSSSILYSDYTDQKNIKSQQYGIYICQDDYRGYDCQIQDLQCLGSICFNDLLFEKICVYCQGHGTCQNGKCICDEGYSGNDCSQYAKCLNDCSNEGTCIQYFPSPQCRCKQEDKRGGEDCSTIFCLNDCSNNGVCINGVCECINGVKGDDCSILNLKFIDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VIA2</accession>
    <name>A0A8S1VIA2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0640156</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8176075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VIA2"/>
    <dbReference type="OrthoDB" id="10328432at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="770"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="220"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="276"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8176075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="843" mass="100637" checksum="670F9CB7A0D4D85F" modified="2022-10-12" version="1">MSFVEQKVKQLRPLRTQRTQSEYDSQQVKPSPELWIQNVLKDEAEHRNVLKSFGIDKASLKTAGVTQADRIYNSLFIYSQGVYNSLEELIAQSFDKKSIMGKLWKVFQLLTEKCRPQVIEIDQAQSQQKITLNIMNDQFQSLMQLKEQDLIDLQNQIKMRDETIHLLQHKLNLQKSELVQYDELIQERDKLFSQETKKRVSFESKINQIQCIFNQQLQLNESLKLKMLNAEQQIHNLELDLEQKRQTILNFSVEIGEKDQRLDNLKCRTFDSELQVKLLKEMNQQLEYKCQEYEQKKQKDYSLIQQLNYQNSINQSQYNKLDTLNKQLTTNYEHLQQRFNEQNTEIQNKTQLIFEQQNKITNYETQIILLNQENESLKNKLQEITIGYQEINKNYKNQENEISQLKQKFSILSTENVQIIIQKNQLTKTYEECMITYQELKKEFDFEQELKFKIEFDLKRYQDQLKQLEQEKKQQMDQVKEMQKKQMFQIQQNDEKNKFQQNQIVKKDETIEELKCNIKILNTSNTTLEGQVTSLTQNLTELQNKYSSEIIKRQELEQQLRLLGLENKNKQIEYKKMEENLMLHQIKYSQIKDYPDMYKEINEQYGIMQQLVEQLQRDITLLKQTKQCQHASIQTNEQIFSNIESQTEIITQDQSQQFCANQEDIQTQTISIKFNTKQIQTDLSQGLPTEDNSSCYDKIIGKKQHQFTQVDLITQTELQMDSQKYTLEQDVSSHHRSIQNSYNFEDSYQTRLQPQYEDLIYDKQQMKLIRNGKVNQNNKIDEDPSQEQNKSYMKSKQKDKLNKGIELPQIQNFGDKSQIVPNQKTPSARFAKMYVQASEYYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VN12</accession>
    <name>A0A8S1VN12_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0700125</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha chain contains the catalytic site.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000070">
        <property type="protein sequence ID" value="CAD8178824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VN12"/>
    <dbReference type="OrthoDB" id="290150at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14132">
        <property type="entry name" value="STKc_CK2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000059">
        <property type="entry name" value="Casein kinase II subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000088">
        <property type="entry name" value="Casein kinase II subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045216">
        <property type="entry name" value="CK2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054:SF0">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="35"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00059329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8178824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="330" mass="39026" checksum="AC131D72E7EB2B49" modified="2022-10-12" version="1">MNIKPRLYGDANLKKRPSYFEFENMDIHYGQLDNYEIVRKIGRGKYAEVFEGINVNTLQKVVIKALKPIRQKKIKREIKILQNLNGQNNVLKLLDVVFDPASKTTSLILEFINNTDYRVLYPQLQDQDVRFYMYEILKSLDHCHSMGIMHRDIKPHNIMIDHEKKLLKLIDFGLAEFYFPNKNYNCRVASRYFKSPELLLDYQYYDYSLDIWSLGCLFAGIIFQQEPFFHGQDNNDQLDKIARVLGTDDLMNYLQKYNISLGPNFENVLGQHPKKPFNKFVNEDNEYLAKADAIELLQSMLIYDHNLRITAKEAMLHPYFQSIRKNSKRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VP13</accession>
    <name>A0A8S1VP13_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0680187</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000068">
        <property type="protein sequence ID" value="CAD8177945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VP13"/>
    <dbReference type="OrthoDB" id="305577at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8177945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="325" mass="38354" checksum="7B31909A3428EBFD" modified="2022-10-12" version="1">MINYLQQSDSSSFPFTERRISHLILNQSNFETKSKTIIGDNFRRTIKKNSLLHSMRLLKEREIDEPTPIATEKQLQTKKFINLKPLKKAIEKITLMRQEQLSRRKSAYGEQFGEMTAKQFQNLPTSIEQHKQIDGQRFALTSFADILERKKRKRTTKKQSILRQLSKQESLVEYDHLERPPSCKTPPKQIIIKQQLKSQLELTKEKQFKQLDQKMISFLSGKPAEVKEFVQRKKAYTINYQVTTPQSSSITGNHRNSTLFPSLHINKLDRCQTNYYESKYAPQLSNRQIQSRVRPYITYNRTLIKFIKSEIKNYSQTIQKSIYNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VXI6</accession>
    <name>A0A8S1VXI6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0740205</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000074">
        <property type="protein sequence ID" value="CAD8180602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VXI6"/>
    <dbReference type="OrthoDB" id="310810at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8180602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="95" mass="10618" checksum="7DEAE63B0E54233E" modified="2022-10-12" version="1">MGCNSTKSSGEQLVQCEDIKRITKQSSELIEIQQNILKHLNKQQCQKRTSVTSLQSTLSSSKNSRLSIQKSEMTLSTIVLQTEIGPQQLNKKLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VXL3</accession>
    <name>A0A8S1VXL3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0750192</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000075">
        <property type="protein sequence ID" value="CAD8181083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VXL3"/>
    <dbReference type="OrthoDB" id="308278at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8181083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="209" mass="24872" checksum="6187AC04851F1FDE" modified="2022-10-12" version="1">MQNYSSDEEISSIPEDEYQDYDRQDFEAIQFADNKQNISEADEEDDDENEENEIQEKDNKLQKNGIKFKLPQRNLIENKISQSNLNYQKVLTETTNQLKNISKIENRPSTSNSTSYKPIYTTSIHQSQQMQKKYNYKQKSFNTLPKRSDPVARYQQMSQQWKKTKFLQNTNGMKEGRKLNLDTRIEQQKQELKESGMFHRYIAKSQLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W0A6</accession>
    <name>A0A8S1W0A6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0780073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W0A6"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8182197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="191" mass="23103" checksum="CD3E4650A364294D" modified="2022-10-12" version="1">MKQLQETSKQIKNLQLFNPYRQIKNTQKNQRDDGKIEKQAFIVQIKLKLMFLIYSLKCTWLLILMTLQKQIIIQFTSYYLCLIKQGFNLHGNNYLFISISNMIISKVEDCYLNIFALCNLIVLQIIQYLQSKQYFLIQISMSEKRHFFYESKFVIKQLYKMIYKNDVIYKLNEIFGRGEKKRIIKENYQVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W0H6</accession>
    <name>A0A8S1W0H6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0780152</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="302983at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051647">
        <property type="entry name" value="Mediator_comp_sub12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007:SF8">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="703"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="871"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1358"/>
            <end position="1430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1897"/>
            <end position="1916"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2242"/>
            <end position="2397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2695"/>
            <end position="2728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="703"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="722"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="739"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="874"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1410"/>
            <end position="1422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="2251"/>
            <end position="2277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2294"/>
            <end position="2305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2315"/>
            <end position="2324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2347"/>
            <end position="2358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="2374"/>
            <end position="2387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2388"/>
            <end position="2397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2701"/>
            <end position="2726"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8182353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="3221" mass="378069" checksum="82954C8B4852F4FC" modified="2022-10-12" version="1">MKPSQFLYSESIKEQKAQKANVTKSIGELKPLPKTANVPKDQNSSMIYSLVSLPVKEGQNDTVVFPQSLISQSMNIKPQVVVGKNTEIARSLKKLKPLDSLDATPESVQPGTGKGVGGEQQQQLQGPKLQVYLKSLNQLTNGQFKKTQTQLLFGSIEPKLNNNTVLCVLRIQKLKFFNEYNQKNEKIPSLFCWEAIYRKKSLMKTTPKEFDAAQQDLLDFVYTRNAYEPVDIILWKIKNNDEKYVGQFDISNNDYQNAREILDFKSKIICETQIEKEMFKTMKQTKSYIGNVQFQLIQLGHEKIPLNKHSGIPGSPRTEEEKARHRIIDAAMSGQEYRWYIDEHDKFVLERIDKVSQEVVAECTLEVVADECGQLHIIYEEKYTDPDKYGRSRPTSRYMRKQHADATMALIKELDKNGSFTEVQANMRNLSRQGRLTRKVIEEALYTMGCTANQLLLTECSEFGDCLMDFLLVTDDLSGVLEAMQELKIIGEDDMNLESFSNNLFQNIGQHLRKNTNLKSVIEFIKTLEKKPEVGLLIQNYSYAPENVLSETPPFLQNFDHKNYEHVMVLSKLNKMIEIFKVDTKFTRALKKAHVEAKKSIYFEIPCFPKNKDLDEKSDSLQLKSIGQNIMHEVVRRQKWNKSHIIFENYSEWFFVADEQGNTPFSRMLETAPFDVIKSLFAAYPDQLKNIFSKFDPNSELFEGRVPKKETKKDEQNNDNNGGDGNDGGDGDNGGDGGAKQKKEKENKEPSEDDLYNQGKEREKARKAFEHLRMQKDYKKNMIHCLLQNPDLNQSDLLDLLRNIELLVNESSESNFETLKSQKLRAGDFTPLGLYLEIMRSKCKKKLDRVQREIGTSLFICQMLTPNIDEEENTQQQEGQEGQEQQEQQQEQKAKSSFVWDSQNQLVISQIIGKLPETLFQSFDDLFRFIKWIPPYNELKIIRYVNLPYRQLIKHKQQQKLFAILQQMYDEVKQNQNYDPTDVESEQNQKILYTIQIWTLMLLEIRNLRSQRLEQVASQLLSQLREFTQQYIELLVAPMPTNITSSYYPLQHQYDCSPSTILELFNGIKFNILLTEFPVQSLDKLDIKQLAKALSNCKDKEPLRDLIRLVLTKDQGNENAGAEGEGEGEKQQQEYNRKLFEFIVDPNVIFNDEDGSYKDLLDKKQVEQLLLTYKAEKILPLTYGKSSNFSENLHGIILNSFQTRKSVGVTQQQVALWIRNNETKVDERWKQRYVDFFRQNMFMQQIHTLIDLDIPKPKKTYPNQNKIQPYIFAQGKNWASKLDVRRFISAIKDNIQYINIENTLRSIALNGQYEKLEQILPYVKDAQMIRQTIHLVATIANMQIFDPRGYLQFRQYRRPPTKDSQDPEELNEGGDNNKPDDGDGGGDGDGGGPQLVSIQNQSEEKKQQKKQSQPQSQQQQQQNKKREVAQEENVNKYLNIASEFQVRCYDPTVYFFKTAIKEKKEKYKVDIAKFPEALDQIRTPISIQSQLVEMTQSIQLEFDDFFRTHFPKSERLDIKKFESNFEKDILMFRYLKNWKSSLQRQNFRSVFTQYDQGTGQQSYKKVLNQLLKQYQRYGMPICDTFNEFEKTLDVLMIYGIQNMDQLLFQVASSKLTPIEKNKIFLNLVQRVINPNNSVLAQHYQIKNANIYLQQINRIIKSVNVKLLQPGEDVEAFQRYILQNIHRTMKYLKPDMANDQKVIIFKLASEVLQRFDQLDIKQLFEQELIEISELIKTRQIKDKNLQALAAKYIKNKEDTKKLSSKPNYKDIIASFKKRRLDEVLSSNDLIYFFKNYSDVQCEEIVTELFKLPYDKTYTTTLLEKFNNRLFECLMFYRRFRTYTKLINDVLFKQILKGKEIAFIPLLMKASGESKQVKKQEVEQEKAEGQQEIAQARAINEGEDGVEQQQDGKDEDSDIKDSLGQVAANFCFYEYFDTLHRFRIKVSNVEPCTSFRFMLHTNEQLLQQATQFEQQGYTYISNCIRMFRYLRLNHYNSDKPSIKLENELMVCFISQNSQKISSPCLNIIKDYLEYSEHKAPFWQNTISQRKFQYKGDQANQRAANKSQQALTRLKVNGIEKGVKFCFPSVYQPDGDNEITNDDIRRTPVKRPAFTIDQYVEHLSPKDPQRLDNQIVNDPEFLQLLNKLLIPSKQLEFLIDKEAFQKNIHQLIYNDHEMYYYLLNFPMFWYEEKLNKDNQIFIAFLMEFELGLIFIKSTLLFEPKLIDQALDVYFKIVQKHLESGKQEQENKELDQEENVEDDKSEYEDVLVEVTDDEAEQEQNQNQQIDKAPDQQEEQQQQQPVENQQLGLQSADQRPGGDEFKEEVQQQQDDDGAPQARAISDQARISKPPQQKPVQQTPAEQVPGEQAPGEKQQGKKTKFVNQKRKKDKGQNEKKFVESDESLRKQIIKGCNTQLQFQIFLQLLLFATTSEEIFRLIQKYFIQYKVVSSPSIPLEIDPVIKARLLKDGFKEPFNLQFVEQAEQRFKELKQQQIEDIKHVKEVLTQNENKRHAEQSHIYQRLIQSILQTKFSGHQAYIDKLLDNPAYKCLLKYKKSVASNTLEKDQKLQMEIRKFFTIYYSKQLSGDFLQKFFENNRANQNQGSFTNDKWINVICLLKYCRQIVNQCQKFKKDIRFECEKLNQEIKTLSYYEEDEFVNPQFIVQSKFHFQFKVSNKLEPIIYEYKKVPIEIKENVKENKESNENQQDNSRNNNEQITEQNTQSQQVDQKVIKKKQVNHQDNFYFEVTFNIQKSSNPNNPYPLAQLYLKNAEEVIKSIETIYYENYLEHINNLCSYPQFKVAQLSGSDNIDYIQLKTKLTKENFREYQELHYSKNFNLDQTIMDLEIYNYLFESQDKMNHLKILKELRDGIVQTESNDSEIRVQICNQVWSLYSSRIRPDIKLKYQDKLYPVPYSNYQPRQRNTQYKQSIQVDQSFPELYPIVNLQVYQYIPDVMTEEQFNQKYDQLSIIQQACYDLNSYAQSLDDFLTQSPSEEELQNLRDIAPQIVSWPLIRCKVKRDKGQTEEYKIIHSPESHPLYMIEYLFSKVQIQLNHQLKFTFNVLSFTEIILFKLTRIIQQTTGKRCKLSTSTSMLNEWALLKKILLKIGDLILENELDKFRFYYVEINEVHQNSVFPIKSNKLEGMDAIFRFGQTQFYVHSNVLIIRLNIAVVSENASSLITYKNTKLKSYELCPLLFKEDKELRGYMNYIITLEDMLKQIFTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W2B5</accession>
    <name>A0A8S1W2B5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Amidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0780104</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the amidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W2B5"/>
    <dbReference type="OrthoDB" id="6428749at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004040">
        <property type="term" value="F:amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017064">
        <property type="term" value="F:fatty acid amide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1300.10:FF:000003">
        <property type="entry name" value="Amidase signature enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052096">
        <property type="entry name" value="Endocannabinoid_amidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45847">
        <property type="entry name" value="FATTY ACID AMIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45847:SF6">
        <property type="entry name" value="FATTY ACID AMIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001221">
        <property type="entry name" value="Amidase_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="3">
        <location>
            <begin position="102"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8182259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="612" mass="68488" checksum="1A570F9E579021F3" modified="2022-10-12" version="1">MKFQDFTKMNQKNILYGGFALVLSSGFSAWALGKFIGYLNHKKMARICKRKRQATIEAINNYKLNHQPCQLSQDIQNQILNGNVAQIKQLLFEGKTTVQQIVLVFIERILKVACSDKLNIITEINFNEALEEAKKQDEEIQQDKNIINQYALFGIPVSVKETFIQKNFDSTFGLGINCFKPAQEDGIQVAQIRQAKGIIIARTNIPQVAMTFESINHIYGTTKNPWNPNRSVGGSSGGEGALAAARGSVLGIGSDIGGSIRIPAAFCGVYGFKPYSGRIPDYGEAKISFAAFGQMQLKISRGPIARCVDDLIVLTKVLFDKEIYSKIPYQIKDPYFEPQELGELPQKQKLRVGYFDIFNGLITPNCMSRAVHEACQALQLQGHEIVEFKVNKQMQSIIANCFLKIVVADGGMKSYIEALNGQKFIEEYDLLVQDSNTSLGIKRFILGPLFKLLGQKTLLEYNYNSKVDVYQYLVDSAIRKQTNLQFCQAVHDQGIDVIISPAFGLPAVKHGDSKELAFAALYTWMWNVLDFPAGSLPVTIVQNDQDLEIKGQENSIDLFYRFMKSDLQGAIGLPVNVQVSALPNKDEMVLRVMKEIEAVVKFNSKHPYPELN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W2R9</accession>
    <name>A0A8S1W2R9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0800004</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8182957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W2R9"/>
    <dbReference type="OrthoDB" id="420945at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050498">
        <property type="entry name" value="Ycf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE SPINDLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13431">
        <property type="entry name" value="TPR_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="23"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="57"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="91"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="134"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="168"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="202"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="245"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="279"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8182957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="400" mass="46670" checksum="D7C4CCE073FE94EA" modified="2022-10-12" version="1">MTIIKQSNLIQMMQQLINLEVNTITLNILAILYDDIGDIDKALSDYNQAIKLDPNEATAYFNKGILYTNVGDKDKALSDYNQAIKLDPKYVDAYFNRARLYKEIGDKDKALNDYNQAIQLDPNEATAYFNKGENQQLNILAILYDDIGDKDKAFSDYNQAIQLDPKYVRAYFNRARLYKEIGDKDKALSDYNQAIKLDPKYVDAYFKRAILYKHIGDKDKALSDYNQAIQLDPKYVRAYLNRGEKYYFNILAILYQAIGDNEKALNDYNQAIQLDPKYVRAYFNRGNLYNDIGEKDNALSDYNQAIKLDPKYVDAYFNRGEYYNTQNQAFYINILVTKLKHQLTLIKLSNLIQIMQKPIYNKYEQAKIFFDKTIQCDPNNGDAYFQKGFMVNIFQQFQNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W4D5</accession>
    <name>A0A8S1W4D5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0840235</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8185228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W4D5"/>
    <dbReference type="OrthoDB" id="10297939at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="229"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8185228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="404" mass="48053" checksum="7E29417F41ED4BD9" modified="2022-10-12" version="1">MFPNIIQPEIKPRSFSLNVDQYGVKQKAKRNISHYSYKIVEKLHSQPASNGIINIVPQKEPSGSLIIIIGNVEAFLKEIAQNHKYQQFLKFKDFLNEKQISQFFHYISNVLINLQGSMFEHHYCTFSLNKKVIDIVTADSKTVYEVITEFLHQHQKQHENLYIYGGDFQLVHKSGFRQIIYISEQRIEKQLNKDQKINEKVIQFRTQVQSQLNDTNSDDILIDHRVYQIHKKNKEIKQYLQQQNNEKRDLNQKQRKSKEKQIKKISSAQKEKKIDLAQELPQICDQIYEKFKRLFQIEYISVDKEVFSQFLLLCELSIYYSKNLTLQQQISRINDDNEKFRLLIKNVLYFEFKILKSMNLNLQQLQNSVTQTLLKFINQDTYGFIFQTIQNAKKHSIKYIGMHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W4R5</accession>
    <name>A0A8S1W4R5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0810141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000081">
        <property type="protein sequence ID" value="CAD8183717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W4R5"/>
    <dbReference type="OrthoDB" id="284128at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035735134" evidence="1">
        <location>
            <begin position="20"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8183717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="270" mass="29892" checksum="A4187B90483F4E22" modified="2022-10-12" version="1">MSKTIILCLVFLSAYQTLSTKNRCDCVLFSNQTECENVPYCSWSNGACGLKNCANIILERCETFFRCSINQDGQCESTGDCSKYTATTQIDCMFKRGNCAAEAIQTNGVYKCRSYTAVTCASQTSENCKNSFENENSFCWLNQQGKCQSYDPNTCSGIPLEICSNLGCDASGTECKAYTCGSNLTVNTCILANDGYFGAKTLCKWNTTTQKCEERSDITELTQNQCSLLTDGGFYWLDDKCTVCPNEDNTDDTSYEILLSSLIIIHLLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W6I1</accession>
    <name>A0A8S1W6I1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0820125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000082">
        <property type="protein sequence ID" value="CAD8184072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W6I1"/>
    <dbReference type="OrthoDB" id="290239at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8184072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="252" mass="29608" checksum="17B322A252C92128" modified="2022-10-12" version="1">MSVYSGFATRQLESQYNKLITSLLKILQRRIIKFYNNELADEKQFKRVVEETLMKLNQMEHQKYNEPYISAIMGEICDYLQIQQNCATGCSCQCHLQIRKQTFLTQDKEILSNPSSCRKQTVSSRKTSVKGINNTSVIEKSQFSKIQQFTQLSSTYKSPYYLSQNNQSYGNSRLNFYQNTSNITHNSSIERPTTKSYQRPQSKSPGRRIPSRIMRSSEGQRANPNESLEERRYEKPQNFGQQYNKFFKKRNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W7K6</accession>
    <name>A0A8S1W7K6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0840241</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8185240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W7K6"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8185240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="103" mass="12300" checksum="C1ECC7DCDB529DB8" modified="2022-10-12" version="1">MTIQQYILSLFSNLNLNQGNIILLSTLQQILHLIQQGLMILRLQSIQTWKRYIIMLEFQNSLNMIKVPNWMFNIIGLFFDDCNFHIFPEKRNRNMNNLKNLAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WGS3</accession>
    <name>A0A8S1WGS3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0930107</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000093">
        <property type="protein sequence ID" value="CAD8189248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WGS3"/>
    <dbReference type="OrthoDB" id="301635at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd19756">
        <property type="entry name" value="Bbox2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8189248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="406" mass="47225" checksum="7FDD2BCF40134C74" modified="2022-10-12" version="1">MQVLQPINDHQQPVKITSEYLFQEAPLTRCIYHPQYIRHFCKEPNCLMPLCQDCIQVHPHQHSRYIDSIESTLTQVYKGYASRANEIATHQTGDILQIRQDLHKLIDTIVDDVLNKADQNYQYNLIVQWRNLLKKLEKLKDPNYCLKETILYFADQDNQNPQLKYQGVPIQVNYEALEQAKFHLHHLFMVTLEDGEYFFRPGEKTGDNPIRRVSRMIYDPVLSTHADYQPYNYSPQRMMVSQPVQQIPQQIIYQTQVAPPVQPQQPPIVQIVPQVQPYTQTYIQQPQPLSYTQHPPQPLSTYTQPPPQPQPIVINKTEPPQPLVYYQESPIKMVSPKQEPVIQQITVPQPTIQQQIPQPQIIQYVQPVVRAPQQTIPTAYTVSASKPFAPLQQSGEYSHPYPLVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WHK1</accession>
    <name>A0A8S1WHK1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="6">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0900206</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="6"/>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8188097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WHK1"/>
    <dbReference type="OrthoDB" id="289038at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061136">
        <property type="term" value="P:regulation of proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044635">
        <property type="entry name" value="UBP14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43982">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43982:SF6">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="3 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="8">
        <location>
            <begin position="118"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="435"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8188097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="862" mass="101740" checksum="91B32D0A3C460219" modified="2022-10-12" version="1">MGTNKQLNRSFKLTSTIFDQLLQQYSQIGFKKTQILQAWQQCGYNQQTFHEELIKISEKENALIMNEEDLFNSQIQLKMQLQQIEQQDLKIAIQLSLDQNVQQKPPITEERINKSTPVGLKNLGNTCYMNAMLQSLFNVLPFRKFILELDLKNNQSLASNFLLQLQLLFLMLQESNQSYINPYNLFDALQKFKPSAMFIRGVQNDFNELQNSFQDAIEQALKEENFEQQKKQFCEMFYGEAKEILSYKENDKEIIKFNDTTFGSISIEAADKDLEKGFLNTRVLIIDEYETENKEKTKAKIDQNIVQAPFLISFYINRVYYDPKQKQVCKNNTQFNFNSSLNINQFKTINQGQKNLELQKLDQEEQRILDQLKNFGDTKEEIEENFNKVIKLFKRNEGQIDNLVTIDGFCICDESTANPNIIQSDQMLFQLESYKEKMMKTVQLLTDQLERIQEQKKKLLKSDQNMYLLTSVLMHDGSANSGHYYCYVLDKKDLTTWWKCNDSTVTKVDFAQVYKDASGSNRVNSNVSGLVYEQEQWLKVDKIQINQKLLSMIQEDKEKQLIAQDEQKVFNRKSIILQQIQQQKNKQLKYKDYFDREFERFSNFDQFLNSVNQNVYQYYLILSTIQQDILLIKDQLNKPQEIDHYIKYFITQTNLITINYDNELKKSKDEYLMVNSLLQQLPLKNITNQNYSEIIRYYYTILKSIQEIKSCSTITNHLKKIYGVLLLKGCFFVDLSLQTNNISTCIEISQSLLIQINQNEIVDKLIFQQVFTNLKLSSEQAKLITKKPEIEDQIIKIQKKGELLINLKQLEFKSLESEIPQLQRLRNDVLKLWIYQYDKIKTEQKLLDQNERQNAEVLLYFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WKL1</accession>
    <name>A0A8S1WKL1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0970050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000097">
        <property type="protein sequence ID" value="CAD8190654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WKL1"/>
    <dbReference type="OrthoDB" id="10310359at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8190654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="115" mass="13533" checksum="537ABB5FAB31B69A" modified="2022-10-12" version="1">MKDSYSVKKYLVQQKQQSIVMNNTLGIMNNRFHKKAILSRKRNTSADTNTIIQIQKGNKKNEYYERWLTNQKQSQLRLDSESNVDNQTPQTPKAQIPIKFEPKLIKDLVQKFYGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WKM6</accession>
    <name>A0A8S1WKM6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0870009</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000087">
        <property type="protein sequence ID" value="CAD8186326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WKM6"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8186326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="112" mass="13445" checksum="03E36050C8591AFC" modified="2022-10-12" version="1">MLINFIKIQLILKQIRTFQQQSSLHDKDFSFFLIPFLNPLMKKQTKLMLKYLLKKSMLVCLKIYFLNYSLFKKIDTTSFQIGDPNYLLQWHTQVPLMISIKQSQSTIDQFYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WLQ7</accession>
    <name>A0A8S1WLQ7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0960173</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000096">
        <property type="protein sequence ID" value="CAD8190543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WLQ7"/>
    <dbReference type="OrthoDB" id="10344390at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="129"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8190543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="367" mass="42468" checksum="3AFE1A572672F775" modified="2022-10-12" version="1">MINLIKSIFQKKSQQQIEQPLQQQLDPYKHFNYKTINKNTIKLKSDCSAIAINKENSLLLVSHESKIKVYMLKNDSLKQVQLITNHHKRVTALKFFNKYSLSYLSSSKDSNIIISSSYLCANPKYIQKLSGNSQGIISLALNIQEDLIVSGSFDQMIRFWSRDTQSTISNWKCSQTIQDHSCYVEGLSINDESNTVVSCGFDSRILIIQRKNLSSKWLITQKIQVHTFCHQIIFINNNTFAFQDIGLTKLLIYQNINDKNQSPNYIKSKELVLQGNHNGNKSYFQSIYNHSKQVLICKNGNTINFIRVIKKSTNQKRDNLIFRLEQAIDYGQLFFGLLVGTLSEDGQYFITWDELSQEIQLKKYQEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WN14</accession>
    <name>A0A8S1WN14_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Palmitoyltransferase</fullName>
            <ecNumber evidence="10">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0990006</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="10"/>
    <dbReference type="EMBL" id="CAJJDO010000099">
        <property type="protein sequence ID" value="CAD8191398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WN14"/>
    <dbReference type="OrthoDB" id="430659at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF301">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="202"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="11">
        <location>
            <begin position="125"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8191398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="282" mass="33081" checksum="BC2B85588DFE5B5A" modified="2022-10-12" version="1">MIFISCRVTLSLIFQILSTLSFFFLIFKGPGILCSTQINSEQKVQVFTPQGDKQFHFTDKTNKSAAKILQSSLVIYKRKHLNSIKEDESIKEEKQIEIPSLIQQDNSQIQEKQQKSQPQSVVYLEQLYCIACFIDQILRSRHCKICGACILAYDHHNFILGKCIGEKNKFIYIITLIFHSIQVILATLYLETIPFQIANIIYGVKTLSILVFCYLTFLLLYQIYLIVTNQTQAENRFRYKLSYMKYVKDEIRMPFNQGFKCNIKQFFISSFQKQPYKWAFVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WN75</accession>
    <name>A0A8S1WN75_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">P-loop containing nucleoside triphosphate hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0950044</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000095">
        <property type="protein sequence ID" value="CAD8189925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WN75"/>
    <dbReference type="OrthoDB" id="309500at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="268"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8189925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="319" mass="38156" checksum="EFAB705B49C6C9D4" modified="2022-10-12" version="1">MAEKNIYKILALASASAITFTSASAVIIYFGIKLYYLKKEMENKSKKHQKFQTLKYLLVGPPGVGKTKLFKRISNFFKQKQVNQEFEECQIRHDIDLVSTPSFDFENINEREIIIKQFSQYMMKNHIIHFFLLVNFERTDLMKKKLLDVLKYFKKFKEKFTVIVTDFQLSDDQEEDKKNLKQALHVFLNKNQSIMFVENDFNVEEFRDQLLVEAQALNLTDTIFEPINENEQQQLLQQFQNMFTNRDCNQIKKQQIYENNNQQQEIIITNTQQDNIQQQQNIQHKQQENEQFQINDQQLFQDDHSILMNDSFKSNQNQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WNZ1</accession>
    <name>A0A8S1WNZ1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0990194</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000099">
        <property type="protein sequence ID" value="CAD8191738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WNZ1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035902810" evidence="1">
        <location>
            <begin position="16"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8191738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="114" mass="13069" checksum="22857056D128E9D2" modified="2022-10-12" version="1">MKLLLLFFLLGFSDFQTLPASNANAKFSQNDLYVLSEKSIIRGHYKDVASQFNGALLSQARTTWYWEQGADNSPLRNLLYHYPEPVKQGHVVAELGESMIVYFLQAYEINTVYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WSF7</accession>
    <name>A0A8S1WSF7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1030037</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000103">
        <property type="protein sequence ID" value="CAD8192964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WSF7"/>
    <dbReference type="OrthoDB" id="303168at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="91"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="145"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="21"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="106"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8192964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="191" mass="22645" checksum="D797B82C7747A270" modified="2022-10-12" version="1">MENLDQNSNTDDRNLQLSQLVLQQKREKILLQKKIQTLEKQNKILQQQMEQCKNEKEDQSNQIKQLKEYIAKQDSVTTKLNGLIKKLLQRDEENQQYDHSLSAPKTDAKSNKYDNQERKKKDKEYLEENQKLDLNEQIIAQYKKHSLKDQSMSSKLPPIEDTKNKPIVVQVPKSQLKKINPQYLIEQLQKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X3P9</accession>
    <name>A0A8S1X3P9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T1100035</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000110">
        <property type="protein sequence ID" value="CAD8195611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="311182at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF13">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="44"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="332"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="599"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="709"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8195611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1361" mass="163996" checksum="49844688FF1FF485" modified="2022-10-12" version="1">MIIYLVFIFITNVFSYFCACMLFKQFQVMNQQKFTIFTSNRLLYVLEEVLGKGSGGSVYRAYKVCNNQKQYVALKIQPYIENSELQTLEILSQQNLNHNINILDLDQYNNQAIIVMDLADGSFHDFWKKSKIDDIQEILCYFIQIVKGTSELHQLSLIHRDLKLENVVYQERNNQKHLKLCDTGLIRQQNGRKTISVGTPYYMSPEQINQSIYDEKIDIWALGMILYEMLAKQVMVKGNSIAEILRNILDLNQNNINTQIDRLYIDDQKYSNEIKNLLKMMIIRESSKRVNAEFVLTELSRILNIKPQEHNNNNYYKYNSILKPEIKEQLRLEFQREFEQKYQQEYEKLNEQHKLEIEQSKNDIIEYYQQELMKQKIQVQRETEENLKALQQRELNILKEEYNKNILNCNQQQIEQLKYQYEEEKQKIIKQMDQKYQLIFQQELKNKELDLQKEMDNQIQTELKNKEQMNKILLKEQFQKYYQEEFEKRSRNLTLFQNQLMSVQQNLNKSQMLINNQLKDLNYQSKLNQSQKFLKFIFDYEKQLKDKFNQIESIHQQLSQLILNSNSQQNQYNSVIDVNIKSLEQIKNSSQELICPNLIESFIQNSNQLLEEIKEEQNQLMIQHEKSFLKDQLVESEKEISQLQKLFQKQNEQIKEVYTIIDSLDQKDMDSIKQYEILNQQYNKFHMEFQIIVNSIKLIREEDLKVEILQKQNLQMSNLVDNVNKLQSNILYFKSKILDQKEREKQMISNQLQNLIETMDDWEQKIDHLNQSQQPYVSEELKEQFEQLNKEFEVLLEIKNRIIQLLNEIQMNQKQDFFKQFSEIQQQFVISIFKLELFEKNYQKEQINSQYKTKELIYVQEEQQKINSNLKQLFESIFKRINNLQNEFQNLSILCNNQIMYQHLSKQLSLIINIFDNYQKIAQDINKSIIDFQKTNFKTINEIKLEESKITIQINLLSQNLDPIFNSLIEIKKQIAEPNDIEWDNDLKYLLNQFEDLKLELFDIKLNERDEQIYNTLYDSKQEVQNNINNSLNQCENQIKQNIDLITQFYSIQQINIQQLNEIKKQQQTSLNKKKEIYITWIEEQKQRMLEIQNQVEKQENQKREQFIIKFIKEYKEVSSFIENQNLKNQISEIGQSKNQSKLNEYYQLAQQYRKKIEELKFKKQQNQSYFQTHNFYTQQNQMLNCLEALFQLYCLTGREKINLEQQKTNKNGQGWGKRQQIKETELQNSIESLNKIQNCFNILNNQQKDFFKIEVFVESIKNYLNYQNEKIDLESNIDFEGQLQYLTKKIFDLIQKKQNNINEKNKVKIKYLVKQDVNFIQTLKTAFNSHIPNCQQLIPPLVGIKELLEQKQKKTQNNIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X5X9</accession>
    <name>A0A8S1X5X9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1050117</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000105">
        <property type="protein sequence ID" value="CAD8193936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X5X9"/>
    <dbReference type="OrthoDB" id="364513at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46043">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46043:SF13">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="315"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8193936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="881" mass="104264" checksum="73F20E1310F60C1A" modified="2022-10-12" version="1">MISVTTKTALSSLFEMGVEYMTLIQHNENRYFIILGNTSIFFLKEGFDILEAKVSFQCIDRLIVSSQDIYLLQIHFNAKRPKNVPLKMNIHSLERRDLIKFIQQGWKTDYMHKFLEVRELPTYKGKFRDYNYQVTMLKKPQVELMENQEMYKYNMHMLNGYNIFFPNSYQNTKKGLYRNGQNKSQFTLQVSETNELEVLEHMHNHIDIQYYAEYYVHNALAEEEKYWITHSAPYFKRRNLYNDLAEWKCWQTRAKVIMKHQDEPQQGTKKKRAAKQIIEMEYAVIMMRRKYHPPYLENFVDIVLTFLYDPKCKVEDKNPEAEQEQQELEDQLQESENEEDGEEEKKNAGEAYIAAFKQLYSDFYWLNIMRDAADSIYASDLKPMDPIYKGFIQLMADSLVFDEDWMFYVQQKHQITPKIREILVIPIIQGFKILFNKSDNEKELDQNKRPSVILEGFKKSTKNQFSELKLSDKQKQEKDRIYIYKVSRYLASQLDGGYDSSFKFKTIMKAHNNYKDDVLKIFDFCLYSVSEQSGVSNDFIIEEVSKKFPKIAYQKYIFNERVMISFLDTDLFKEELLKKDQQQLYIDLLMHLLIHGKTTKLATCKHIITYHSKDKEQITEQIINLQKGLISPLLIVYQSDHPMLSTYACVALYNMCANSKEFKYQIMKENGIALINSKLSTNNQNVLLYTLKLIFSLMTIVQNIEAFLQLDIMNTLIGIIQKHKGFALYSAQVLAMCFKIYTKCISRDMDLRDKLDLFFQVVVLISDVYFVEIKDVDFLKAEAINTIFKICHLNIEDEKYLEKVQSGLMPYIIQLLQVPIEYELQQSIVKLMCLMIDKRLSFRDQWEVQTIVPIIEKFESHDPPINGADFLLKQLLLSDNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X7J3</accession>
    <name>A0A8S1X7J3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1070176</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000107">
        <property type="protein sequence ID" value="CAD8194816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X7J3"/>
    <dbReference type="OrthoDB" id="287782at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="484"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8194816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="543" mass="62934" checksum="5ACC4B52A0E885D5" modified="2022-10-12" version="1">MLLILIQVALAERIFTPVIRHVYEQIPHTFYSPNVNSTLLLQYNSYPGVYSMELGEIIDGNYFRIHPNVTLDPFTLPGCNQYERRLIYKHLANQSTQSPNAFLTNMYIDDNLVFVTRSDNSILTFEIVLDILDRSVNDLKLKSIQTTHGSSLEHHIACFNKTCAVFGQDSAFLYKDPDFQLIDLEKNIQGKGSFPIKIYFDEKLNALYLIYGTNLVEVYQFTETSLIRFTSFTAGQQIVSVRTNPESTMLYLLDNQAGLLFYKILSIGEYVDTGFMIALKNCQQFDFYQNTFFLVAETTDSIPYALEVLVNEDSYYFNKIYSKDMDIFDVWVGEHIAILIGSEVHRVIYHSVYNKFISESETPLFFQDIDLMNVEEFRKWESTQYTKNAIEQDPFSKTSFQYKQFFMVGISSQQLSIFSIKSISPWLQCKPISTNTTQYYLKMNATNCPTKIKENDHSAFKQCIIMHNFTLAGKEIYFYEQDQMTVIIGGCVLIGFVLLLIIAVVCVKKYLDNRIEFYILQEKQHVRLEELEPETIPSENVIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X7L6</accession>
    <name>A0A8S1X7L6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1140121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000114">
        <property type="protein sequence ID" value="CAD8197115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X7L6"/>
    <dbReference type="OrthoDB" id="294627at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8197115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="290" mass="33741" checksum="28637606E8BD979C" modified="2022-10-12" version="1">MKQQQPYFIRPPNLSSRHSTNKISFLNKEDTPSNSQGVRRKTPIIAQPLHSPSQFKDLHRINPNKRIPTLNSPLHDGSISKYKIINDLSVYKLPITRRIKSDDQTSKIVPTKKQKSQHINNIKLRTDRRKNFTPLTKMKTEPNEIATHSITKQDESQDILFRLHKQSIPTEPRDKFESILGDKIVSGIKKIEKKTENFIELEEDSQFFLETKQNKKNKEIPILTQETKERILDLLCLSTQDLKKKFSEQNRIGNSKINVRQPNKLVIPRNGKSKFPLDFFNALLPNSIFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X981</accession>
    <name>A0A8S1X981_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1160014</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000116">
        <property type="protein sequence ID" value="CAD8197601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X981"/>
    <dbReference type="OrthoDB" id="538223at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002141">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="35"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="77"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="119"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8197601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="242" mass="26628" checksum="0DA3F0432126A50C" modified="2022-10-12" version="1">MSVCFSPDGNTLASGSGDNSIRLWDVKTGQQKAKLDGHSGNVMSVCYSPDGNTLASGSQDQSICLWVANTGQYKAKLEGHTHYVYSVCFTADGNTLASGSGDNSIRLWDVKTGQQKAKLDGNTSTFCSVCFSPDGYTLASGSYDNFIRIWDVKIGKEIKHQNNNTKEILQKYTTPLLQNNPFTNFVNPNIPILVISKNPYFLSNGALIFQGEFYTTWGEDLRAFFESRGGCILENVNQIKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X9U5</accession>
    <name>A0A8S1X9U5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1160116</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000116">
        <property type="protein sequence ID" value="CAD8197794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X9U5"/>
    <dbReference type="OrthoDB" id="294569at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="84"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8197794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="302" mass="35842" checksum="23E68E6BCC9BFEC7" modified="2022-10-12" version="1">MKSRKKEKCLIKELDAIRSLKNESIDLKELEDKMVKLIEKAAQYYQDQLDNNMAQYYFEYGSILVQILEQSQDSVENSLNCVEISRNDRELKNTQLAFENLEAAKTILRNRLDKQNIEMYKLQDQLQQIRSKINETELEMSLILIRIGDLQTWREDYKDGLLNFYAALDFLKNSYNYKELSAVNFKIGNAILLQNENGCEKLSLNHLEQSFRYCYQQLYNKQCDIDLSEKNLQKIVLDNDLLKMLIDKMEDTYIQLNEVNACQKEIQKWKNQEPIFQQEIQYLGRISKQSNEINGIQIKRKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XAS5</accession>
    <name>A0A8S1XAS5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1170113</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000117">
        <property type="protein sequence ID" value="CAD8198119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XAS5"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051826">
        <property type="entry name" value="E3_ubiquitin-ligase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765:SF434">
        <property type="entry name" value="GB|AAD18119.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765">
        <property type="entry name" value="RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23106">
        <property type="entry name" value="EGF_Teneurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="341"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8198119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="393" mass="46615" checksum="41BA6993C63FFE41" modified="2022-10-12" version="1">MQIDIGYQPWEQNFIQRKINQTLLFKVNKAKQLRYILIELFVITRKHQANILLLQSYQEAPCFENNSSRKQIRYENADIYGKQQLKKYHYIVLDQENKTEFSQFLVILTNQTTKYKLIIQNLSQMPCPNNCNNNGICKNGVCLCNSGYLDHDCSQKGLQITPFQQFQQKNDNEMLEYIYMSFNYTLSFNMLIKFSKKWENITDVRVQILISQKFNLPSDSNNTYDDIINDKKIAKYKIKSNINLIDNEQIKNFTNQTYFLLIKIISNKNAKCCIQIEFDEYVDEDDEKLIKLLVILSIAVIILSCFGIIAYRCFRQKSDKQNQNRMYSNPIQEDIQNYQECPICLDNFEINQLTQLAKLDCNHIFHIQCLTLWRTYYTNTIYSICPCCRQAME</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XCR2</accession>
    <name>A0A8S1XCR2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1200089</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000120">
        <property type="protein sequence ID" value="CAD8198957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="194865at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46118">
        <property type="entry name" value="PROTEIN ABHD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46118:SF4">
        <property type="entry name" value="PROTEIN ABHD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="28"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8198957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="276" mass="31855" checksum="281B25DDC448BC9F" modified="2022-10-12" version="1">MQENLIQIDFSQIESLDXQDYNIGKINLVVLHGFMGSKTNFKNLVNSTQISKHLKSAYLLDARNHGDSPQTQTMSYEEMAYDLKHFIVNHNLDNVVLLGHSMGGRIIFSYLENFPNDRPIGNVIVDVGPGERQGKNYVKKLQEIDLKNKSLKDIETNILELVKSKEMTKLIMTNLVSTNKEETNVNYVWRLNIDSISNFQKQGNKEFRSTYDGPVCVICGERSEYVNYNDRDRFLQVFPKIDINKDIHFIAGAGHWVQVEKPREFINLTSQYLSQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XIP1</accession>
    <name>A0A8S1XIP1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Carboxypeptidase</fullName>
            <ecNumber evidence="6">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1260109</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="6"/>
    <dbReference type="EMBL" id="CAJJDO010000126">
        <property type="protein sequence ID" value="CAD8201023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XIP1"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000408">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF113">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE CTSA-4.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036517749" description="Carboxypeptidase" evidence="6">
        <location>
            <begin position="17"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8201023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="429" mass="49956" checksum="3483CCCA53B523B1" modified="2022-10-12" version="1">MLLILIFGAFIDASKGLIYSKDQTNFFKNLLPSVNYYIETGYVSVGDLTISDNRLFYSLYLKENILKKTDIKADDEFILWIYGGPGCSSQDSNYNENGPIRVDDDQKLHARETSWNKQAHLMYIDQPFYTGMSYSTKEGLIDNSWDAADYVIELLTQYFEQNKELAKARLHIWGESYGGHYIPVLAEKIKKQTNFNLIGIGIGGAWSHPRVQVIPTVRQLINYGVIDQYRYDLMMKSGIESLDDIDNQEYEKFLTVSDEEIFFNDAVGPNFRYNIQIYNYDDGDQYEEFINDHKLQFDLPLSITFYGCNQDIYYAFAEDQTVSVLPSIDYLLSQEIKVFVYNGQLDTVVSHTGVEYWVNLLKWQDLPIWKKQKKSIWKFINPKTNREETAGTIKSYKQLHFCMVYNAGHQTPTDQPEACFQMLKNYFDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XJ18</accession>
    <name>A0A8S1XJ18_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1260026</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000126">
        <property type="protein sequence ID" value="CAD8200859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XJ18"/>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8200859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="255" mass="30225" checksum="C3C48268D6D71B70" modified="2022-10-12" version="1">MDEPQVVKIIVVSESNAGKTSLLQQYCFKGSIIIFQQQQVVISHQSGQPQMGLNQSCRFGMWLGCLIMCDITNKKSLEAALSWRHVVKDIADEVPIFLIQNKTDLLQGEREEYQTEEYLNQFGQQNNFTCYQISVKLNEKVNLIFDELVEVMIDKGVIKLEKSIIKRDLLIKMKVHLQLKMNVMIEIKQRKKNVVENLYHLYYQFLLIFKKHKINSINNNYYFEMNFINQKESQQFIIYFDYKNSQLKYSNNYRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XKK9</accession>
    <name>A0A8S1XKK9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1270066</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000127">
        <property type="protein sequence ID" value="CAD8201288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XKK9"/>
    <dbReference type="OrthoDB" id="300482at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035722375" evidence="1">
        <location>
            <begin position="20"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8201288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="429" mass="48821" checksum="5323A688CAAEE30A" modified="2022-10-12" version="1">MISLIFIFLITIYAQPYVGQYVCSTLTSTTCGTDAINGKGWCYWDTATTTCLTLPCYLIDEVAACRTGGSLQTGGANTDCDSLETFSLQYDNVCCDKNLGKLNYAFVRFTMNFDGYVDQTNDTPPISMASLDITTSVKHMFKLYTINPWLVSKETTEVNFQTKLIAILDKYLETATRNELLTQSRSHPFYLERTVYQALQLLRDWTVMHQSTITLRNAILPKIRSVALVALTRMTKFQPNYYQTNYYFVNFAIIPYFRNSLKISGQLHTLTIDWSDYAYTDNGYVMVYSYAPEQFGIIRAYSDIVCIRPFLGNSVTYVDTENNYDNAFSQIKFTYYWSDTTQTINDQTKVKLFKISDDTQTITDTLATFNCVAATRTCTISANYSPSPTPTDGKGQNYFIAHADYNTAQNEKKRAQCILAYKTWTTTCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XQA9</accession>
    <name>A0A8S1XQA9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tubulin-tyrosine ligase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1340113</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000134">
        <property type="protein sequence ID" value="CAD8203666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XQA9"/>
    <dbReference type="OrthoDB" id="202825at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070736">
        <property type="term" value="F:protein-glycine ligase activity, initiating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051437">
        <property type="entry name" value="TTLL_monoglycylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45870">
        <property type="entry name" value="TUBULIN MONOGLYCYLASE TTLL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45870:SF2">
        <property type="entry name" value="TUBULIN MONOGLYCYLASE TTLL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8203666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1003" mass="121084" checksum="12B8E6AB03217F90" modified="2022-10-12" version="1">MKQQITPKQKITTQLPLLPEIKRQSKDSLETPLKLMKQAIQNPLSQSHLSRQSCSIETIISQEQQRLQSQTKKQIVEPLVKNTAYFSNRSLMNDYLKRQNITLATKMFCFNSQDEYVRRCLQRHGWLEISCNQQIFDLKWVYTENPDDYKILQDGQFYNHFSNTKELTTKSCLLSNFKNQCEYGYDTSTFFPRAFDLGNITDREDFIKEYERTAVTIILKKAIIALKLKRKNEMKKIKEVIFQEWKKKEDNLKIRRIEMKKTVKRKYKNIYTAQELQDSSQASDIKFDCSIMSEVLSRLSDMKRQMKDGFYDDKNFELSHQYISKSRINIILYSQIQKFDKGQNPSKFLIRKLYRYHLFLKKYDSAYKVDGVNNIWIIKPGGCARGQGIYLEKDIKEAINSGQQMQARLVQKYIERPLLYKGFKFDLRQWVLVKSFQPLQAYVFSHCYMRMCSQTYDIKDTKNLLKHLTNFSLNKSEFKNKNDSIYSSDFMQLWLPVDWQKIVRPQCNDLIIKTLKILQDQFESESKYCFELFGFDIMLDEYCKPWLLEVNLSPACSERADWLHEMLDSMAESMFNIIFGDEFIRPKKYYYELLIDEQQTYNNQQQNDNQLELWGQKCNIKREKMIDKRYIESLAALMIQKFYRMYKAKKIKWFLRDTKYAIVIQKYVRRMLSKLLRIRLKNYYSARKIQILIRGFLAKKRKYHLKRSKMAIKIQCRFRYNQSIQIKQQLKRELALLYLEQLISFKISSKVANQLKYKLHKYKQIQRWWKSLYRKRIKQSIKINSIIKCFIQKKKYKKLLKNHTSSIVIQKNYKRFKAIKLRKRLEQEQSSILLQCLMRIILAKKQQKLCIIQNGLNKLTKIYYRHKSRVAMFNIWYIGFQRLKSIMKIQSFRLVIKSKRYVRRLKRLKKFIRLQAFIKGFLQRKRYRRLIKRKRAIKLIIKCIRKKIKKRIQLKKGRGLNIYSQQPSKTNTIQTISTRNSQIIQQFQSKNIKKQTIQISNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XQS4</accession>
    <name>A0A8S1XQS4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1330182</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000133">
        <property type="protein sequence ID" value="CAD8203403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="284918at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="242"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="288"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="347"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="389"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="421"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2263"/>
            <end position="2283"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="1466"/>
            <end position="1495"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="1532"/>
            <end position="1561"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="1720"/>
            <end position="1759"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="1760"/>
            <end position="1789"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="1916"/>
            <end position="1946"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="1947"/>
            <end position="1977"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="1983"/>
            <end position="2019"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="2020"/>
            <end position="2051"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="2052"/>
            <end position="2087"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="2118"/>
            <end position="2147"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="2148"/>
            <end position="2177"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="2212"/>
            <end position="2244"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="488"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8203403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2318" mass="271239" checksum="427C868CE2E28871" modified="2022-10-12" version="1">MQFESPGFNMDLDQELQAEQHEDFEQQYLGFCLRTTKRETSINCPNLYSQTQSIKSMEGFNDLNGQKMENITLKSIQQNTPETNRTLINTPTNKQHSTGSSMMAFFLIKRFLEKLQIKRRIIETLNFTHLNLIGDKAADINVVLQYTKSIQRAGFTLKAMKKLLKMESSIEETKMETMKQYYQNMKKQAIKILQNFINKIPLIQSESVFKMYWDLFAVVFRIILVVLIPLEVAFKTKIMFESNFALTVIIIIILIMDFLIRINTQQYLNGQAIRDRWRLIVYQAKKSMLIDFLSIMIMIIFLSIEPQDQYYNLFTLVTLTQYSYVYEILSKSEQLSYFTRPQRGILGLLKFVATLFYILHLFSCVWFWISSLQIEDSWIDFKDLTDKSWQLQYLEALYFAIVTMLTIGYGDNVPKNPTEKIVTIIFILGACLWFSYSVNFIGGIMNDITQNQVERNQKMRVINKYMTKRNIPFALQHQIKEYLTYRWKEDDEVDLEIEQALLDQLSDELKEELDKQAHKVFIEKSILLQQFFSEEFRNALFKSIKRKIIPPENTFQIDFNGQHHLCFIEQGHLLYQHKDSKQRSKMNTIINLGEFLCVKEFIIEDPEMELFKAVGYVSLLVLSKQDFLQTLKDFPEDFQKYCQLKDSIILNLDSIVLTKSVYCPACQQFEHSLAQCPYIQLKSNREVVIKRYQHSKYQLRSSFTRRTSKNVFLALSEKELVAEFAKVFSSDNQVIINQQLKIYLNHESDLFDSNSVEPPPSNDNIKLTPQVQQGCLPPQSTACADLLSSIRIQKNLIRETDIDLKGELRQQLNQGRIIRKMTLHPQNKQIKKSTTTTFTVRYIQDEDLEDQQMAIEFQNYQNENIILLYNKLVKQDQDEPIVKQALSQIEPLFWKLNQKTIDNFEVEQKYTFLAKGYHRQAIKIYVFPFQLHTQVSQIKTQQDESSNYREKQQVQKNKEQIKNDIIEEQFTLKENVNIIKQKHKIQLDFAIVVSILRFNHYLISHVKYIYIYIQFKLFKHQFIKSTRSNKIKQNKTLFTQPNQKLQRLNYQLTNYMIVLLFATFKLIQSCRLLFNQLGEYHCQTCDTNQDFCSSCPSTRNLSFVVEKGANANVCLCKQNFVDIGGQECLQCPSNCMDCDPNDPSKCISCGNEELTYRVLDSNQTCICKEGYIEVWYTYNCQNIQFNQQILQFEYILSLEQYVNRLQNKNTYSQMTIEGLDYMIQRGIVNDGINQFQLVSKLESNKIISTTTPYPQGQAYMQKFDNLFAFGIMLNHESTMIWAANVNYINYNQYSGIRILNQSIWEFVIFQFLLQKYEGNIFLQIALLENKNINNDKQNRLLETKEGKMALCYYLAYELNNFLLESQLDEIINDILYILLQRPSNSLDYFYNGQELIIQKCQPNYILKNKECLCDNENGYYVYNNICQLQCPDDCITCTKEVCLLADHSIRCQENYYLDIQKNICLQCYQTCQTCINEKECTKCKNEFILSNSQCLTCQEFQEQYKCDQYDQNCLCLKCMDGYQLDEFNQCIECSQNCKSCHLKDQCNICKDGYYLSDNQCVQCHENCILCQDQNYCIKSQKGYYIQNGIALKCINNCLDFLNECQCQDCFDKQYRKDIDLFCYPCPVNCQTCSNQQTCINCVSNSFYLDISECKQCQFPCLTCLSQTKCLSCIDEQHYYIKEHQNCAQCPTSCKTCYYDENPLCTSCQQNHYLLNQQCYECPLYCKDACIFDTIKNFIQCEQCQIGFYPTYINIPFECQRCKEYCTNCTNEESCLECSLGYILNNGKCQSCSQLYQNQCISCNKQLCLQCQNGYKLNNGNCIEDKIIEVPICLVNKQYYNTISNSCDSCIQNCIICKEQQTCIKCEETYYEKSEICIQCKFPCNTCLNDQFCLTISNQLYYIDNGITDLNQINSFRCKSPCNKCYSNEECIDCIDGFYFQNNNKCLQCDSMCKTCKNMSTFCTSCNSGFLLLNGLCYQCLDQNCQNCNRFCQTCNHFKDDLYKCQSCLIGYYLRNQECHQCNSICQSCSQSSDQCSSCFEGYFLTSEMKCEKCNIGCKSCQVQTKSCILCEDGYYLSEISDFVYQCIKCEQGCKQCQEDVCLVCTDYYYLTEERKCQQCVLPCLKCTTLTQCTICENGYYLNNEMCEKCMQNCLTCSSSNNCLICQDSYQLVDLKCIKCPNNCITCNEGQCLQCEQEYILQNDDYCHKVSIICNINQCQKCFDVNQCLTCSQGYTLNTQNDECKIENQIFDSESTDKDDSGTIFIIISAIFSLISFTLIYLIIKCRKIQIKIKLIERTQPKFPELPVTISEFPADEQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XR81</accession>
    <name>A0A8S1XR81_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1350143</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000135">
        <property type="protein sequence ID" value="CAD8204006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XR81"/>
    <dbReference type="OrthoDB" id="406844at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8204006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="128" mass="14975" checksum="F5186D642D56D89C" modified="2022-10-12" version="1">MNSQIKHQLQIQLQSNQDHQQQFIKSQISQVQQPQEQQIKSLSNIKSFNYQIINHNYIQQQEYCNLVAFNKDGSILAVGCDNLINLYEFSQGMLKYNQSLNDHQDTVNTLYFMRQSNQIISGDQNGYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y265</accession>
    <name>A0A8S1Y265_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1490090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000149">
        <property type="protein sequence ID" value="CAD8208009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y265"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8208009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="159" mass="19600" checksum="59971A500591E9B8" modified="2022-10-12" version="1">MKQYNKFIPVYLEICPERLYKRISSNQHIYCSLYKCILKKFTFYEQVLPNHKYGITLQQRYQQETFLLDSEKEFRKWFLLFKRYFFLDKFKQKFKVLNRSKMTDPLFGQCYYNCIHSNNYKLVKLIDKTIITNYQLQCLSKAISNLRKLKNYFNNIVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y5M8</accession>
    <name>A0A8S1Y5M8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1520040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000152">
        <property type="protein sequence ID" value="CAD8208801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y5M8"/>
    <dbReference type="OrthoDB" id="292259at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="377"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="266"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="377"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8208801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="397" mass="46925" checksum="269DEA04629723F8" modified="2022-10-12" version="1">MLVNQRKCMFCEKLRNDGSFHLILKRALYCKYSQSQNFFYEKDINDIIEDRSVKCTIHYKDHLHFNDQNEYMRRYYRFFESDDRLPALLEYYKYHINIPRNFSSKTINKRMERNREYQYLKIKQELGLCQNPKNIKQDSPRKVKDTSEDQSVSQMKNLLKDLKIESTQTDISSNSTILKDLVKMIGNNAQKPQPFQILNYNKFIQKQINPVIEAQKLQCKQLETNLLKPKENKTKLIKSPPLTERNLRSPQLSRQSSNTKIKNETQKQQYQQTQITTTIKRPPQLKSRQASLGNIELTKSCQTTARNNPQQNELLFELAKKVFSTKIAINNNLLTKKKPISQQTNNFFVKGRSTLQFNLKNMEEIKSSIQSLNVRHKTPNQLIKSQQGTPNYKKKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YBZ9</accession>
    <name>A0A8S1YBZ9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1630002</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000163">
        <property type="protein sequence ID" value="CAD8211289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YBZ9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8211289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="142" mass="16907" checksum="F72632F9CCA22992" modified="2022-10-12" version="1">MFSKGIAYFGRLIVLFGISYLKVNIGNILIFLFPQLLYKNNQQQKHQDSELNTRLMGVNQQKQRFDIQSKNQRKLFNMIIYFGFIKIQIPKVFFKSNQLMYQQLIVFIQISIHKLVTCLKFLVIEQVFFAKGMIIFIMELIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YEC0</accession>
    <name>A0A8S1YEC0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1640034</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000164">
        <property type="protein sequence ID" value="CAD8211548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YEC0"/>
    <dbReference type="OrthoDB" id="304932at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="413"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="505"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="414"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="511"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8211548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="684" mass="79937" checksum="24E4C71D6CBB81E8" modified="2022-10-12" version="1">MSVIFEDIENLHYLQFQFNQALRIGKLIYYIENSLLRKVNVIDNNCTDSVIVQAEVSMICTDENGDIYQIEQPPRLIKSGHHYKWISPIAAIDQSHRGFIWKTNKQIYSHCKHISNYQDRITLVTIGGKIFNVVDNKTIPIPQLNGLKLNTYFKKSILFSFGGVAITESGEFYLFNQKMIRVKIKDIQDIFFTDKYIFAIQNNNTILQYTIEEIQIHQFFTNQQIFQKMHYCNDVEFKYKSRQFQHISILQSEYSLCSFAYIKENKILDTSLNLTDKTNLTHNKSNVLIMKQSLFEEWDCKKKIIDSRRSTTLDKQPQIESEGQTLAQKLATIRESLNFEKPQQNQIDNDKQDRCRQKSLIQLHQIQVEDLPQNPTPIKLDNLLNKIHVNKPEKPIDQLLLIQNKQELQDSLPTFQNDNQFTNNNDTLNKKKSKQQNGIQRSNKRLDSMSAQSGIDLQNNSGFIQQQDSFVDILDSDSIKKDYPLQLQPFDKSNKYKKNTLNTIQSYENEQDPKTTNRLSIKKDQILQQSNRSPNKKTIKDPLITERSTTNIEQAKQDLETLVNMINKQKENIEISKSISTVKSSIRMISPPIQVGSALIRPERDHLLLVKHLNKNHLNYLKESQNKIYLKNQQLNNHSIHQVKILNFNLILITLDNQILYKIKNHNQIQKVLRNSLINQSLNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YQD3</accession>
    <name>A0A8S1YQD3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Insulin-like growth factor binding protein, N-terminal</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T2010006</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000201">
        <property type="protein sequence ID" value="CAD8214232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="304690at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="902"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="999"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1089"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1130"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1152"/>
            <end position="1169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1181"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1221"/>
            <end position="1243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8214232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1300" mass="150719" checksum="CF4C91537CE63A29" modified="2022-10-12" version="1">MLLICILISITNQQTLFYSAFSDTILTQTDNWRFLNINPSNIITQCGSEYIIGGFERFAQSQAATKILLLPPHYRMKISMTLYIIDSWDLNEYFQVYADQNLVYNVAYSGLQGTGHICGQQQTYTKDQIHNIQFEFDHTGLTTYLYLTSTLDNSAWDESWGFKNFKLFLYLCPPGCLVCIQSDSEFDCKSWVLTHTAYTEIDYTLFITDGWTISYGDVNKQQCFMIPTICGYEVCGKDTVLSLNLINIPTHSQMKIKLKYLKIDSWEVSDYVQLKVDGELILDQQLSQLNEYLYGICGTTQKEVFVNIELTFPHSITSARIKILNTLDQAFSDESFGIRDIQIFIKQSLCGDQVVELQEECDDGNLFPFDGCFNCMFSCVDGCSECRDMICLSCYEGWIYLEYEQICEKEIIQEYSLITILELQTQFDCFPPIDNCQVCLLGKCILCQNDFFFNQFNDKCEQICFDQINSYYELCDEYQLQPFCNRCQIKCNDHCLNCNQGICQVCENDFILENNYCIQIGKVNLCQPQCQICIDNICYKCQSGQLLILGQCQNICGNQIALYTLEECVCDLYCEECEDGICYQCNNYFKLFNNQCISTCGDLIVQEHEDCDDGNNIEFDGCFYCQYSCPIECIKCEQGICQDKCLPGFYLINNICSTICGDSIIAGNEQCEDNNTQEFDGCYLCQFSCPLNCEECINGICSNCNFGFKLIENKCTNVCGDGIMSNEEQCDDGNQESGDGCSQICQVEIDWICNQGNDCTFVKYPNLKCEYMMQKNQYQYAQIKFSQAVQMLSDINYQNTIELSIIDLNQTLYAITIKEIQSAQYQVTTDVEYIVQIEIFTNLPNHPILKVKLTEQLFSANLAPLVNMVDYLQLNQPNYTTDQQVYIAQTVQMISEISVKSIYAISIILILLGNVLSFWGILDALQQQSYLKFINVLYPQTLIIYFQSSELISVQSLLNSITDLNKKAKLLQFPYLESYEKFQYYEVNADITEGFRTEILVSLTLLLCYICSILLERVISILELSSSLQGFPKFIRFLQKQQRKLNLQIKKIDISFIKNTLLACSWDLIFMSLLELYSDHDFSYYRSYVRLSITFVILIIIITLILSQISGVTPLKKQDLNQFWLQKQPVFLLIKKISIISILVFYQREQILQTLLMTFVNTFYLIYIINMKNAEEFQFYIKNIIMETSLTLFTASTFLNWDILQRYLSYNFIITISWIQMFLLVSVLISFLIFELYNLFYVIKRKITKLIEKRMSKIKSNEQQQSQKLDNQIQNANLEFQKTIFQRVSFLKDKQIQIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RWL9</accession>
    <name>A0A8S1RWL9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0010308</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RWL9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8131832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="62" mass="7786" checksum="EBEE082808351883" modified="2022-10-12" version="1">MFKQKQLNYVKNCRQVKRISFIRIVDKVLQFYQWQTYAQIDNAQVSCLRYDARDSIWLYWKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RYA0</accession>
    <name>A0A8S1RYA0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0010613</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RYA0"/>
    <dbReference type="OrthoDB" id="354826at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000454">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000854">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF374">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="24"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="11">
        <location>
            <begin position="283"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="361"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="367"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8132442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="381" mass="43781" checksum="845FE9E59B7E65DC" modified="2022-10-12" version="1">MGNCNINEKQEDQLESNITSISNFQFIDAIGRGGFGKVWKVKKKKNKLFYALKVMSKAKIIQKKSVQSVINERQLLSNLRHGFLINMQSAFQDKEYLYLVMDLLTGGDLRFHIGRLRRFNEEQTKFFAACIIIALEYLHQNGILHRDLKPENLVFDSSGYLRLTDLGIARIWKPENSSDTSGTPGYMAPEVMCRHNHGIAVDYFALGVIVFECMLGRRPYLGRSRQEIREQMLSKQAAIKRQEIPPGWNIQAGDFANQLLQRKPQNRLGSNGPDEVKEHPWFKDFNWQKLENKIMIAPFIPNCNEDNYLPSDGRKDSEDSLNQEQQILLRRNSIQDLFKVYDYDSSQQQPSINNLMIVSNQSSARLSKPPTSSSTPKSSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S1G3</accession>
    <name>A0A8S1S1G3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0020044</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8132734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S1G3"/>
    <dbReference type="OrthoDB" id="436703at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="38"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8132734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="146" mass="16388" checksum="681767FDE28A4CFB" modified="2022-10-12" version="1">MSNAFKSAAGNTLSKNDMGQTGQSFAGSQNPASLKGKLMSLEETIKGIQDEMNFHKKEVQILKSEKDTLESVLSMKTQDVKKTLTNELMRIEEEMKRHFAHQKAENSRLQQQITALKGEKTALQQQLLGLQRRIAELELQVGQEQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S539</accession>
    <name>A0A8S1S539_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0030465</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="296485at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8134722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1769" mass="212156" checksum="5741819C8E711FFE" modified="2022-10-12" version="1">MKQALLKLRDNKLLEALSYFEQHKPFEEAAQILIKYSLTQESNIKELIYRWILRNTNSPNIKLDVYNLLGCTLVNKDQQIEILNEALKLTVAHELNPSTTLINLTCIYSKLKQHEKALQIALQSIECSVGKQLVIAYHNAAIEYKALLQNEQSRIFHEKAYEQSVYLFGQQDTLTQKLSKYVEKLPPKIVPVQNKFRLRSASRQGEDAKSFYYRRPITIIDSYTQSLNQSINQSDDSNYRSGSQIKGRRQKTLNQSFQTSSNDIDEEEGSVVSKQLQNKIEAQRKIRLRFLQSIHQQNIQLLERKLTKRNSYHINFSQESILNVECQDQRSQDFIHFENQQPDGQYDSMIPLNQQEDLTYDDQELIINQIRGFESQKEITASFANQETFENTFEQKNKQGKAISKIQKWYRKKQKNHSVKYYSNNSKIKIQAAIKIQRFYRKITQQRLMKLMKNKKYVKYIKISKLSIENEYEYCLIIAKKFENEILYIINEMKNKRQRKKAKIYRQTFELEILNSKYRAFDEFCLTINMSKRMNQIAYKQAQQINYKEICNLFYVLNNELNLYGVTDTLLNELIDSDIDEKVSVSSRRQPIKLTKLMKLQSLLRGYLARKKQELIKNQKYKHKRFIEKIQGGYFILIFQQENVIQFYNLQQKQLCENEMIIPDSVIKFFGPINRQNCSEIFYAQNNKIFFTDDAQFIIDKDIRSKEKMDFSAVEKAAKKIQRVLRSKIKFFDSSILKYKTITLKAKQSQLLFKIRKSILFEQKYHIILTAYGQIYQEHQELLIEGGIIQKKYSNLKIAQCCQRLFQDISISDFNQVSKIVLDEIDFEWKNDKLHLKCHSLEEVYGFLMERNYSSFKESLKDVANKEPTFLGRNYLSRKIYCQQYLKNRFEIEDRPTIFCRVEDPSIYQITYIKNLYLERLKKKYQQKKKERQVGDNINHYVNQYPSIQKYEWIQDPDELYMEMLNKKAKLVQRAFRLLKFRAIIHQKCLIRRRRRLALLQSQKVVVKPYYFDEIRKPQAYTTIEIIQRRGETPIFLKSIYIDSVLYEWILILPYYDIIVTVYTIPRQFYQIKYNEYKNHYMDIILSESVEKIQQYFSQPIEKFHPSEKIDFITELREQFSKKVIRIVRLKLFLKKIKETPQSKKFLIRTFVNEFFVQINYFYNKVDQKIIILLQKEKRKRKYNLDLHYLDCLFGNQHGLSYLDKELFIKLLTLNEPKSYHKYRMLLMPMINVLHLYIMINAELDLQYQYKNFEMTLTEEINEKFYTSSQGFYMITNESDSKLLPTIISLQARWRLCQQQKKYQLMLFRCTRMKIKMMNKKGKEVQKTFLKRKDISDDKDSDIMYICSIYQSKDNQGVRVRVELISYKSKQEQELFNCFTIEPQSILLNEQISQYVLKNLYIEDNILLLRQNYTQKLEIPQETQQKQLKLITKKPSSPKLIFHQKRQYSQGKQFIYDAIHTGEMPQQQKMTQEIRNMIKIDIDLKELKSQYPGVNYKQSNILSQLSEQLISGLKVDKQAIISKSIQKRGIKIQGNCQNEEKGKELNISESTKQQSTLLSRFTQFYNNLRYMVVISLLDIPIDSYEKLDSVYEPYHSYINITAQNQLNNKRIIWQMNLQQANKITNQQDLKEIAQTIKFNILVLEDKFVYVADFAIIEFDNVLMIKRNTMNVIQRMLKNKKFNRLRKQFEIEIKQLKESDNQFWNNIIYYGINQFSSEDYFICIGIERRQYLLLAWNIKDYQRYTLTMYKKKGFQEQLDQIRFINQNLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S7C6</accession>
    <name>A0A8S1S7C6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0040325</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6350175at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051197">
        <property type="entry name" value="ECM-binding_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33150">
        <property type="entry name" value="EXTRACELLULAR MATRIX-BINDING PROTEIN EBH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33150:SF1">
        <property type="entry name" value="EXTRACELLULAR MATRIX-BINDING PROTEIN EBH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="247"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="646"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="713"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="844"/>
            <end position="1324"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1361"/>
            <end position="1635"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1661"/>
            <end position="1894"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1926"/>
            <end position="2067"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2094"/>
            <end position="2240"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2265"/>
            <end position="2506"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2675"/>
            <end position="3095"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="3134"/>
            <end position="3335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="249"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="646"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="664"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="685"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="725"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="750"/>
            <end position="765"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8135635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="3346" mass="400453" checksum="44DD952EB4A6AF4B" modified="2022-10-12" version="1">MLPTIKSAVETQPILFENEQKQLSQQCLEQKMNIILMATEIERLHKILLSKKDYYEKKMQDLIDREIQDKEQIAKQAQTITELNQQILQKNALITQILQEKSQQEMNLLISQQLFVINEQYKNAEIYIQELQKENQILKQQFEDAPSLRQKLYNLEQWYAKLIHEKNEEIQLLKMQQPHIIQHAPQLNIINQLLEQKERELLQWQYSSQQVNVYHENQRLTQELKFLNEKLNMLLFDNEHLKKRLADIQSQPSNQTTQPQYANLKGDSQCTAPIYHDLQSTKTPRRYQQSIIPSDLTNTSLLSYRILRRPQSVGSGSSIHLQIPQIAYPQFQFSPRYSRRSVQNPSTQFNELLLQIQCIQNQPLLTIQNIETQIQLLNTLIQIKRQELHIQITPPKQIYNILKTQGNDRTQTQTYSPPQQIIMKQPINILQQKIQPNQSVRVLHQNQLQIPNNIIKFRSFSAETKRELSPNNIQYQQQQLIPQTSQFQQVHQIQYQCSPIQRIITQTNLQNTIPRQIQRSVSPQPVQFLQYQQLSPQAPQTVRYQDVNQFHTITHPLTQQYYIQQQQPVNYIQFSQQSQQQFFPKQFGIHSQNPSDQNKSPINILAYPYEYSPQAPEDLNKTKQPQQPMTQNQNTLFMTPESVRKPIISSSNSSQPYSQAKNPIKREEQEPLNKQKQREPKHQSNKSLPAQTIQQSPIDSSQVEPFELCNAPQEPDVQSKEHSTPEQISKPQNPAEQTKPKLEQPIQQPKQDEQIKNKQNPKEQMKNPQNPENLYKQQQKPQNIIQNPIVVNKINQNSQLQSKQPKNLENLKQFTQQPESLRRSQPASVRNSQDIKQIALIQDAEEQRKQKQELDRLKNLLDQAKQDNEKLKELYQQNDLNWQKQFQQKYTAALQQLQLFENKLSIQNKEYNQNLEKQQKNFNQLQQKFNEQKIVIDKQRDEINQLKQDEDEKRKKITTNDEQKDELQKQLIDLQKQNADLLNKLKTQKDNQMQNISKISQQNENLSNNEKQLQQQTQKYSELNQEYNQLKQKYDQLQKESTQSKSAQSKEINQYQQKITQLQQQMNENTKQLKDKVNQLQSQLLLEQEQHLIIKKQQENFQKDYGIQKTQNGEIQNQTNTLRQQLIEKEQQLKEVQTILNTKIQVIQQLEHQVKDVQAQNISTQKQMQSLNQIQVDNLKKQLQQDQLRINELNDQISNLQQKIYTLEQQLQLEKQANIKISKEIQNNKIIIQNDYQQQTQQLEKDNIELQSKIKQLELMLQKERENNIQIQQQFITIENNYQMNSMKSIQENEKLVNNLKFQIEQLKLQNEDLSSQIITVKSNYEIQMSTMNTMASQNVINLEKQLYLEKEQKENSSMRILQLEKQVADFQNIIKQDQTEELKNKVIQLENYNLKLQKEKDIILQSKQRGDEEIKILNVRISELSNQNNQYELEIMNLRSKQNNQTETVSKITTLNQQTLILQQEKQSQNNEITQMRQRMEKNNYDHEQQLLELRESFNRDLENSKILNRKLQDDILNAQNLLQSERREKELLKQNQTHADQIIEQLNHEKQMLHDQIIKLNQQIQQLQLEINKLGLNQINNKDEQNQYQLQIIQYNSQIDNLNINIQKLNKEIDRLNLQIKQMNVQQGDLECQNLNLQQQISLLTSQNQAIHQQSEINVIELKSVANNSEDQIQDLTLKLEQQIKETEKYQRTAFNNDQLVQSLQKKLSEENLQNQKIKQQYEIQIEELVRKINEKEKVDQQTPPKAQIFPQIQNTHLIQENEELMLKIDQYEQMIKSLQEEYYKQQGELQKLSKEYHNVINQSEQTVITITQYQQSQDNIKRKEDQIKQLQQQVQDLQKKLESERNRYQDEINRLIIKNQELSNQMQNNLNKQIIEENFNLKNQIQLLQQELKNTPIKETIIYQSVSPSNKNQEEITKYIQQANQLQLRMNQMASDYNLLEQQTEIEKNQLEKQIKSLQDNEQKLQNENNQQQRNIEALQRQMQKLQMNHPNDNSQELEFELKKLKSQFEITQLQYKQIQEELNQAKSQAFQYRDELVDEQNKKNELNLRILHYEQILNDLKQSNNPESTMIKETTIIKEQVQDPSLIKQINLLTDDNNSLRKQLQNLEAEYIQKFRLQQLQIQELNNKLKEQIEESYQKTTITEETAFNTVSNDQSAEIRKLKDEKLFLQQELQKQQLQYKRQIEQITNDYEFRIEQYKLTIQQLKESKEIQTNNDQNLLKENQMLADQVKHLQNELTMSRALFQDSLQQNNQNSTEANKFNLLQKRNEELDQQIDQQNKIIAQLQQKLQNIQIIYDEKEKEIQNLKVNNQNLNNQLNKLPKLVEPENQTKNDNEYLINLQQQINNLNLQVNQQREEKDNMNQIIEDKEKENKDLTVMIQSLKHQISQLNSQNTELIQKLKNSEQIQVQLKENYSISSSQDQGKLEQQLINKQYEIEQISVENKKLNNKLIDNQNLIRKLQQELMAYGNDKQKLQFQHQSDISTLQNQIKNMQQQLLDQQEIKRSSESIPQKIEINQTNQTLNSPKTKNISKSLNKSNISFHSTDDIKGKYYNCSLYTTNKNGQNYYTSLRAVKSKGDLLASQENSQANTQEELKKQVSLWQSKYEELLRDKYSKTYMITSKSNKQMEEPIVQIESVNWKSKYEQLQEQISNSNTKQNQNEKIIEQLRINNEMLNSQLYQIQTDYQQLKTEISISQKSDFNQNNQNSQRIIDQQNLKIKNQETEIDQLRQKIKQYVESKNNQEKKSSNQQNEYLKQLETENFDYQQEIQSLRIEIKRQQEKNNLINQQINDQDINLKSGQANNEINLEEYQQQIELLQNEINIQQNQIYSLQNQLKQSKIRELELEQKLKYTIEDLEYVQQQKQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQSKIVQTQIEQNNQYDDQYQKILYELSLIKSQNSSLQAQLQESTKTQIKLMQEIKELQIQNERLNLSNVRLHNEQSKVTDQSSQVNRLNDLYKEVQIENQKLRSEISYLQQEKQQIIISFESKLQQYSSSANSKIRQQQIEDLKRELAQLKRATLKSSGADMEAERLTYSTQITQLKKQLEYEQIKNKELQQKLQSYQQYQSDFGLVERLNKELQDKIVELHQVKSEYKNAIQMIHQLEEQVIERMEKEQYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S7F0</accession>
    <name>A0A8S1S7F0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0040536</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8136008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S7F0"/>
    <dbReference type="OrthoDB" id="303141at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="7"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="8">
        <location>
            <begin position="100"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="373"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="205"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8136008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="548" mass="63745" checksum="04449134B21EBB5E" modified="2022-10-12" version="1">MHQELNCPRCEISFDIYDRTPQMLPKCGHSMCMHCLREQTKIICPEDGIVQPQDLNLYPVNQAILKIINKSSLKNSQIDSTELGETVRLSKCENFFEIMESTSLCKEHNKKIELVCLEDHNKICVNCALFGGHKHHNVRNIDEVISEIQHSLEETVDLMQQIQSKIKSLKQPIINKKIRDQIRVCIKQQQTLAEEQFKEMITQLMMKQRKVVEEIEQKYKQVEDQLIKREQQYVNQVVEKADLWMLASQERINQFASLTEKGEIAFFLLQKDTSNTQAKQIIEELEDFMKKFQTRLQEVTQGQITPQVHLSKDLMGNFEYNYPQVQQSFFDDPNILRDVSMIDQSFLKDIDDRPPHNEFLTHNLLNPQPLYQSQSALQSQPSSNNISQNAISPNLEKKSSDILISPKLRRQSSMSPKRPTKKKFNTKVESILLNCNGDTLDLSQQDLGDDGMILLEEFIKNKKLKILKLMRNKISDTGACKLLELHCQILHLQSNVITERFLDSIQQLIQKQTQIHIKTIYLGQNLMNLFRVKKKIEDLKKLGITIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S9W0</accession>
    <name>A0A8S1S9W0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0050381</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UbiH/COQ6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S9W0"/>
    <dbReference type="OrthoDB" id="683240at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000021">
        <property type="entry name" value="Ubiquinone biosynthesis monooxygenase COQ6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010971">
        <property type="entry name" value="UbiH/COQ6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051205">
        <property type="entry name" value="UbiH/COQ6_monooxygenase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01988">
        <property type="entry name" value="Ubi-OHases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43876">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS MONOOXYGENASE COQ6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43876:SF7">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS MONOOXYGENASE COQ6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="7">
        <location>
            <begin position="4"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8136858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="391" mass="44098" checksum="CA4B58172C9F4F0E" modified="2022-10-12" version="1">MQIYDVCIIGAGPVGLSLAAALQKSKQLNKIIILDSIKRPQFNKQGLPNQRVLSVTQSSREVLQSVGAWDKLEQGRVNQYNRMIIDESNGQDYLEFKNQSAHIIEYDNIVNALLEVYDGEIEFESKLEKIDLKPNEYVTVNDKFQCKLLIGSDGNKSKVKECSNIGTYGHSYHQMGIVCTVERTDQDSEVAYQTYLSNGCPLALLPLYNPYSSIVWTAYLDDYKYLMSLSDEQFLQVLNSQLGRYSPRIKSISNKRMAFPLQSLQAQRYIQKRIALIGDAAHSNHPMAGQGMNMGINDAALLANCIIKNSRSGNDIGLEQSLEEYESQAKLMNYTTSIAMELIKNTYENKTISPLRQLGAKVINNLDPIKQIMIQYGSKHPLGPSQFEWVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SA95</accession>
    <name>A0A8S1SA95_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0050317</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SA95"/>
    <dbReference type="OrthoDB" id="307232at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF9">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="44"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="80"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="113"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8136743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="201" mass="22916" checksum="2A32983AC9B5D629" modified="2022-10-12" version="1">MNPISMPQFKGDPLLFHAVAHGNVQEVISLIEDKEASVDARNINGATPLIYAIQANHPQITELLLKFKANPNLKEYYDVGEKTALHYAVEKNQFKLCQLLLDYGANPSLQDKRGLTCLHYAARLGFKQIVVLLLNYGVDINLRDENGFNAAYWAQVNKFNEIMILLPPPKFIPSNDLLEFKTQMREIHKIEIGKKKKKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SAN1</accession>
    <name>A0A8S1SAN1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0060025</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SAN1"/>
    <dbReference type="OrthoDB" id="319299at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8137306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="550" mass="64562" checksum="CB450A0954D1E61F" modified="2022-10-12" version="1">MYLLMNQGHEREYQPHSISVNVVVNLEINNLNFTLSIEPEISFNIFAQKIKEQIFAQRMKAQYEEKIEWQLGNGSNLQENDGRTLQSLGFTNNCTLYVRLKLKKMGAISVNVVVNLEINNLNLSLSINPEIPFYIFAQEIKEQIFAQRMQPQYEEKIECQLGNGSNLQENDRRTLQSLGFTNNCTLYVRLKLKKMGAISVNVVVNLEKANLNLSLSIDPEIPFYIFVQGIIEQVFSQPMQPQFEEEIEWQLGNGSYLQENDERTIQSLGFKNNCTLYGRLKRKNMGFYKKKLYKVLVVLDNIIELFIEQYQLNPVSCLFHDILIELFKNQQLKFDRPFAFFINETEINPFDIRQIRDFETSKGIILKLIVPNKEVNKDSMEKYQFALNFNNLIKPSSRKIIKIEQNNDIEMFAYNDYSLFDIIQVICKDLKIYPYKSINYYINNEQFQESQLKNAYAINNIKVNNQNTNETGGIVVFKVIGDIYKKLKIRGYINDYEYNIEEFMSTNTSMTEFLKYVKFKIFGPKQIDFKQTIIGQDDFEARVSYETIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SAQ7</accession>
    <name>A0A8S1SAQ7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0060035</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="319299at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8137326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="13500" checksum="7C4E3C118CCB0666" modified="2022-10-12" version="1" fragment="single">MYLLMNQGHEREYQPHSISVNVVVNLEINNLNFTLSIEPEISFNIFAQKIKEQIFAQRMKAQYEEKIEWQLGNGSNLQENDGRTLQSLGFTNNCTLYVRLKLKKMGAISVNVVVNXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SEQ9</accession>
    <name>A0A8S1SEQ9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HotDog ACOT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0070059</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the acyl coenzyme A hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SEQ9"/>
    <dbReference type="OrthoDB" id="331699at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03442">
        <property type="entry name" value="BFIT_BACH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000220">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033120">
        <property type="entry name" value="HOTDOG_ACOT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12655">
        <property type="entry name" value="ACYL-COA THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12655:SF0">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51770">
        <property type="entry name" value="HOTDOG_ACOT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="HotDog ACOT-type" evidence="4">
        <location>
            <begin position="73"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="HotDog ACOT-type" evidence="4">
        <location>
            <begin position="264"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8138326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="403" mass="46393" checksum="FE879F7DF65BA06E" modified="2022-10-12" version="1">MLYRKTLQFLSNQVARSKLGQLQYQGIMMKVPCEMQKEENSEEDRLVKAFFTKNHEQIKVYGHSQREPNEKSSSPKNSWYKVVLPFKSNPELSFKFRRFYTNQVRIGRLLELMDYIASTVSYNYILPSSGATMVTAMVDNINFFGKISADQDLEIQGYVTYVGNSSIEVYIDVMQEGVVNVSANFLMVARDGKDHSKAYKVPVMDLNGEGDKETAELRCYLGKHMQEKRKQQKDRGLDRKAPSQEEINELHKFFMNPPSDITIDETQIEKTLLMHIQDRNLHGKVFGGFVMREAFELGWLNAYLHVKGQGFPQIIHIDDIQFLAPVDIGSALQVQSRITAVKDTIMHVEVNCYKISPNQQKIRSNDLHLTLNVPGIAIPQVQPKTYNEGMQWLNACRRMNYTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SFU6</accession>
    <name>A0A8S1SFU6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Enolase C-terminal TIM barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0060346</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SFU6"/>
    <dbReference type="OrthoDB" id="288460at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22962">
        <property type="entry name" value="DD_AtENO3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902:SF1">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enolase C-terminal TIM barrel" evidence="2">
        <location>
            <begin position="187"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="106"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8137892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="483" mass="55883" checksum="75863996F9618035" modified="2022-10-12" version="1">MSKSPYIPPEVKAYIEQYKIEDIIKDAVNTILSQKPADPYTTFSQYFGKLSTPVIELDEVTGLEILNNELKPTIQLIFTVQFKNQKLHFNSPLYFSQAFPEQEIFYDDEQKNMKQAIQEIEKVSLQLRGKKSKDQKQVDDTLMKYLTEHPNRINVIQQISFSLLPLHAHFMNQSLSKLLCQQFNTQEVKTPKIFVNLLQGSKVVGSKCKIYKFLLICDGEGAVQKIQNVVNNVKKTIISGKKGEAGLQFYQDGTFICPGDTIPDNIKIIEQAIDAAQLKDQVQLGLVWLAELFYVPEEKKYDLDNPKKLLDADQLIDYYFKLCQEKPIIVYLEDPIHHSDIVGWTKITNKFKDSKVKLGSRRLYSNIEKAKKHCEILTLQTNPELTEQQINQMNNERINLDYIYKPLYEYNTFTELQNHLTYLISKKSSLQFIIGDSLVDTEDSYFVDIAFSLPNSNINIGPPIKFEKIIKYNKFLKLCHESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJR0</accession>
    <name>A0A8S1SJR0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0080134</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="343921at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140673">
        <property type="term" value="P:transcription elongation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09928">
        <property type="entry name" value="SH2_Cterm_SPT6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09918">
        <property type="entry name" value="SH2_Nterm_SPT6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032706">
        <property type="entry name" value="Spt6_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035420">
        <property type="entry name" value="Spt6_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035018">
        <property type="entry name" value="Spt6_SH2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035019">
        <property type="entry name" value="Spt6_SH2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028231">
        <property type="entry name" value="Spt6_YqgF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017072">
        <property type="entry name" value="TF_Spt6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10145">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10145:SF6">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14635">
        <property type="entry name" value="HHH_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14633">
        <property type="entry name" value="SH2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14639">
        <property type="entry name" value="YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="1286"/>
            <end position="1301"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="1311"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1327"/>
            <end position="1362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="35"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1331"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1346"/>
            <end position="1362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8139509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1362" mass="162307" checksum="B6FE20AE2B19B884" modified="2022-10-12" version="1">MGKGKYNVESSEEEGKNDYEKDDFVVDSSQISSDEVQEDQNDSFNEELSDDDRSLIEENKMVINQRCLQKIEKEYDKVELKDTPVDPFSQEQFDENEQNEKLINKLFDPSQREVAFLNENDEEILKQDVPESIQITYMKMTKGQIMQNAREETSEQQFKWIGEQMFTQFDYKNIVDILVGIKLILEFYRQKNLDIMYIYTYKRHLITSKLQLEHYWKIRELDYEWEYFSNFKNRIQKLIDSIPDEYQKQIDTANMYLEKSKQIEDLQNLKDYIDYIIMKHNCKRIVYLEQELLKENQEKQLLGQRMKQDQAMELEQEQVNSKIDNILKELENLNLNSKKNPHTMEKDMLECIRQKVDQVALKFCYISDEFFIKLQGKSVQVDRKFIEDLEELWNHQIETAEFQSQNSISQYIKLLQRYLMMDLYHHPFIRNFINQLYRDHLLISTEPTQLGNREITVINYFYPVKRIKHRQYIYMEDQIWMQCLRAEQLGYIKINFSISEDKVHQKEKIPRDDIMVLLCDQFANIHDSQKEPENIIRVRDLMLRGILKEILYPILERQIKYKLKSLSSKHIVRMCQQKFKQLINKQPHMSQVGNNQQMLQTTNPDVKIMSLIIGQEQTDSNVFKADSQDCPIQKKYIGMSIIDYKGEQQTLQIFNYLTQDEKRTEMLEQIRKEQALLDNFFEKYKPDNVVISANHSDCLKLRNNLLKKYQQTSSVWITLADSQISRIYSMSEKSNLQLPDIPQILKEAISLARYKLNPMAEVLNLWSDSIDKNGCLHFNQHPLQSMVSQQVLLDALKQTATEIVNQVGVDLVDYTLYKHLADQMQFINGLGRRQAKHLLTTVKGRELMKQPQKMEDLLIEEWAKLKELKDGSENFTLVAQFMKEELENPFFYKFDTSNTEMSNSEIFYAITQESPYTFRKNSIVQGSIIKQIEGKEKCEISDKLLIKLSHNGLYGALHQEDMLDQEFRIGQIIKVYVKRIPATENNDPNLNILFIELSQKLDWYRWDQEYLKPSEYDPWPYSNICYGDYCNKVMQTFDLSKLKEFDQPIILLELPNIQREKLILRNINHPQFQLVLLKGALEYIESQPVGEYIIRPNFRLKDHLTTTWKFHEELKSVQSYKPQLVLKGKAYESLDEIYENYITPCNLHMEAAVNNEKFYNCTMEQLEIKKNEKIIPYGFCVTDKFPQYIVLMYMKQKNKVEKELIKVKPEGLSFHFVLQNNLNDITIWLKKNFNTQENKDYIKRTKQPIPETDDRLNQLNSQWEVKREKTKEERVKHEESSYNFTCSKCNKRGHNANDCRQMRDRGGRNGCYNCDQNGHFKKNCPKLNNYRSERSNSREREKMSIKKNQFQTIGEDNQQIDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SL50</accession>
    <name>A0A8S1SL50_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0070473</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8139104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SL50"/>
    <dbReference type="OrthoDB" id="324666at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8139104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="325" mass="37951" checksum="11DD974CCE8D8FC2" modified="2022-10-12" version="1">MNNLPQQNQKSIPNQNNNQFQQFQNQNPQQFQNQNFQQFQNQNFQQFQNQNFQQVQNFQQIPNQIPIIKPQQLPNQNPQTFQQQINQPVNQFIQINKNQLPAPIGVQIQPPSSGMQQMQSGSQPQGNTIQQNLQNQQNLQNQQNFQKPALVQAQVQKIPNQNIDQQLNNNNQTFQPNNQNFLNFQQLNFQQNTQKQLPNQNLNFNNNQPFIQTGQNQNNHIFQNQNPQVPSPLLQKNQDLQGNSSMPSNAPNIMIPPIQQPVKQFSTQKQYQPVIVRNEDDKKQLVKNLNEYLSKGESDFAIECLKQILENDIRVKLPENFNNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SP63</accession>
    <name>A0A8S1SP63_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0120140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SP63"/>
    <dbReference type="OrthoDB" id="285432at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035903540" evidence="1">
        <location>
            <begin position="17"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8143301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="219" mass="24762" checksum="DBC26B37DE6A3CB2" modified="2022-10-12" version="1">MQKLYILALLLVIITAQESFLEKQKHVKQQKDLSTEIYTIKKKGLPTFLGRSTDESIKEQLSSLLNYEEALLLTPTEETDHTIKFVESMFIIDQPNPNAVRATEKPNYSFEEPKPQKKVYSNEKITSFKEGCIYVFSECNFKGEHAKSCPGDNVTFFGLPFEVLSIHVPEGGSLSLIQENGTTAYTVSNKCMRSRPIEFLFVEGAIKQSFGNGEAFIDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SVD0</accession>
    <name>A0A8S1SVD0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0120083</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SVD0"/>
    <dbReference type="OrthoDB" id="162894at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05579">
        <property type="entry name" value="STKc_MAST_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000604">
        <property type="entry name" value="AGC protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000633">
        <property type="entry name" value="Protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000340">
        <property type="entry name" value="Serine threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE GREATWALL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="828"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="551"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="629"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="713"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="561"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="629"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="655"/>
            <end position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8143187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1256" mass="145776" checksum="7EE62A718E39C260" modified="2022-10-12" version="1">MNKIQFHSLQVIPTKMEFKMFKPKLNYIVKQVHRLKCVMLNKIDIELIIDQIIFIVELLIAGQFGAPSLAYLNELLKILVTTFNSFKQNQLGYVITNMETLKKQTNQDYLKSFKQGSSRNQKIANQLQKIQKRILTIIRIAYAQSFLFWLDEHQPDQSTALKSKRILVETKTLLHSKLKELDLEKHSKIVCRICEQLIEVEIMVAHCITCQKKAEQSKKLLQLNLQLTDASQLAYKLKHDVQLKLGRLSFQEQKQNRLKQKKQEEEKKSFRSLRRTFTTHIEDNKIFQEEEEKNKTKKQLALINSVMTIIVNYTEKVLNSNTNNEDNKLNRITFDELTDAKCNIVTNEINEVALEIIEKARSCIYDRMEYFKILQNLETQQIQESQKIQPYIEIDLKQKYKNSNSSSFRISKFNNATPTSQSKIKQNDTINEENHENLLCQTPKINNNKIKNVMSIINKEKFKTRNSGSANEIKRRDSLTMMSMTRSQKTQINQGTLEEQTQFQSNPASKILDRICNLSNSSDVLMSPISNKPSLFRKAQSPTAEDLVIESSAQTPLRRMVSSDKNESPDNQKICSIADIAQCDSDQELPIKNEEQLLSIDLDRQRDSVEEIQKDKDQNVINSFEKINQSESDLENKSNQAKNSTPNSNQNSNQEMKNSQYNSMKNSGRNIMMNMMMKGSRKLPLYSLKSEISRPLDIDLCFTQSEQIITESQFTASPPKIDNNELSPSSSSSSNNIEIVQSDEINHEEDFDRVVRKPKKSIFHPENSKNIKLKNNMKLSIAVQAQNFKQSFDFEVITVDRGYNSDSEFVSINAEKKTQTQEIGLKDFQFIKPLGQGAFGWVFLVKKKTSGDLYAMKIIDCSQKQLETKLDTLKAERNIFEILSGEFVVKAYFSFSHEQYLCFVQEYMVGGDFSHILKMYTALDEEYVRHYIAEIVLALEYLRSKKIVHRDLKPDNILLDKQGHAKLADFGLSEVGFNNRLKLKLLQQDIESITIPEFADHNDPKFNTIFDLKLPQAQQVIKLSTQNQSSKNKRIVGTPDYIAPEVLKGESLTNSSLDYWSLGVIMYEMLCGIPPFNDDSVEKIFENILNYKIEWPNVSDVEEESISHNAYDLMCRLMEPDYRKRIGHLDINEIKQHNFFNEVDWNRILQMNGLIVPKIILKEGENDNKNNEKVEQFLNNLEKKEIKNQTLTCKLKNQLSNLERLDLLVKLSLEEATYKLSKIQNNQYKLKKLLNKINNYEQKNQTQMFLLYEDVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SZT5</accession>
    <name>A0A8S1SZT5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0140327</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SZT5"/>
    <dbReference type="OrthoDB" id="310032at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="30"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="128"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="235"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8145603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="617" mass="74756" checksum="7CE9CF9FF1596806" modified="2022-10-12" version="1">MSFSQDDELLELNSRYDNIQKLNQNIFIENQKLQHEIQILKQSLIQFETENNDLNDQIENLKNDLRTQFTISQNLRQENEFFENQIHDYKKQIEFAGTYYNQIVQENDLQISQIQQKVQQNDYLTQENHLYKDQIISLQEDNDQLTQELNNLQESINNHENFNQDIKHYQNQDQNKNINKKNRKKNKELERLIIKKEQEISQLYSTIEDLKNYIQQMKYQSEKNEQNLTNLYNSYQQIEIERNKLVSKIDSIQNSNDRYSNQQQQYQNSEQNKNKECQQLKIQLDQLQMYNQKYQQQIQTINDEFTIIIQKHSDLQDLNNQHLQELQILKNELFEKEQFLEKIQQQLQQKQLKEQQLQQQTKEINQQLKELQQNKSDQQTFIMQLQKENQIYSTKLNQLKDQQSKEFKDNIIQIDQLKKERQNLIDLIKIKDENQVELEIEKKNLQIMLKQIQEKRIHLEQTEQSFVVVTQQCMQLQDQNRDLLRQVSNLENDLQKNEEELRQLNRINKNSQNFDKNREIIHLQQVVERLEGMNRQLQQECDRLVAKIQLTSHENTLLSRTNREFGQMGKLLIEGQQIKEMLEKKKYEKPSTLINSKAPQVIQQLQQYNRRPSLKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T520</accession>
    <name>A0A8S1T520_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0160122</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8146928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T520"/>
    <dbReference type="OrthoDB" id="298261at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="375"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="409"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8146928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="434" mass="50628" checksum="DDFEAB4A358A99F0" modified="2022-10-12" version="1">MEIPRSKPLTLISMQNQQEGWLNQTTSQEIDYFEYGQQNDNDSDEGHKIYNRQLSYLEKQSLLQKTNFSDEFNSNNIEMKLLHRCGGLLARQCNIQIEMQSNQWITKIFKDVYLIVQDREFESKFKSQILNSSIVDSINKRIKSNSILLAHDPTGSILLKLASHTRTIYYLESDKTYIDLIDRGIYKMNVNVQKVLKMDLQNKIDVVVVNLYFIDSKHNLRNIMHEYLQYAHDIIIILQPNIDNSLIYSQAEQAISLSEQSKQTCSIELQNILENNNCIAKILYFGGVTEITLNDELNFVYSCLDSSVKKSYNYKLMFKDLRNQMGMTKLIKLLNDFQIIQGIGQFIEFSTNINGYQDKYRLINNQTQDFEYENKNQKDELIEKQSSSSSTESEPEEDQNQERLDFRSETLNQSSNSIQQPPVLRHANSQPFAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TAM8</accession>
    <name>A0A8S1TAM8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0180170</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TAM8"/>
    <dbReference type="OrthoDB" id="312885at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016929">
        <property type="term" value="F:deSUMOylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606">
        <property type="entry name" value="SENTRIN/SUMO-SPECIFIC PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606:SF1">
        <property type="entry name" value="UBIQUITIN-LIKE-SPECIFIC PROTEASE 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="5">
        <location>
            <begin position="333"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="299"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8148647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="551" mass="64762" checksum="D06F53F1E9F1520B" modified="2022-10-12" version="1">MSGSNYDSTINQELSQDYEQYDQLKANLRDNIFYVEPIYYQDQMRDFNQRNPTYGVTVEYKFLRTIQSCDFLESSQLQFYINLFRKAYKDVKLAAATCHFFADFIVSLQKPIQFQQQNQSTSSFQRQNIYQEPQNQQKIVDSQYQIKLKEDFLESKVLDKPIIQNPEQKLAKSSIITKGTNISNETQNSIISNQQTIQSNIQQNQNQKYTQSQIQMQNPLQQDITINKQIQSAIFPNKSKEEGINHDFVHEKSSQISEQQSTINNQATQQGSIIIKTDQNLNQQDINIQSITQKSLLIQPNKEQEKSTINEEQNAPKNNQSSIIQQQFINSNVTTPFQNIDIEKESQKSKLTLDQQKIESTQPVVEHKQVQPPPEKKYHFEITRMTIEKPVQQEKIKKSQLPSLIKQLAEMNNLTEEELKQQDYWYFPINQLQSHWISVVIYLKKKVIYYFDSYYKKIESVIIQGINSILEYFNLNPQNFQIIPVYNQQINGYDCGVFILLSLLYTLQNKEYNYNQKVATLFRKSILYNLAVIGAQQDIEQDYLEFIIEQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TBH0</accession>
    <name>A0A8S1TBH0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Chromatin modification-related protein MEAF6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0190318</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EAF6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TBH0"/>
    <dbReference type="OrthoDB" id="440324at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000123">
        <property type="term" value="C:histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015418">
        <property type="entry name" value="Eaf6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13476">
        <property type="entry name" value="CHROMATIN MODIFICATION-RELATED PROTEIN MEAF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09340">
        <property type="entry name" value="NuA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="62"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="5"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="74"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="113"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8149810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="155" mass="17998" checksum="25672004C0538294" modified="2022-10-12" version="1">MDKKYQDLVEKKGILENELKNLEKNIFDEETRYLEETGHIGNVIKGWEGYLNMKNSKLGGNLQRKGKINPNDRIFSQSSKTSPFIQEISQPVQTTGAQKQILNEGSGEEKKEYHFRRTKKNNKYVRFQKGEGGYHSPMTSSDEYHEKKGKVQKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TEF1</accession>
    <name>A0A8S1TEF1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0190036</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TEF1"/>
    <dbReference type="OrthoDB" id="298514at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8149292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="185" mass="21734" checksum="0BDD6D9C6E5C6368" modified="2022-10-12" version="1">MELEQQQQPVNLKELNLSQISYSSINVDPQEDYYGGYSGRSLELNIREYQENNEFKKKNLQIELSIVDYLSSLNIKMKTDPKAKQDRFFSNLRNQCKRRALTQLEIERDFSDNTPDTIIKSSQVSIKINTLFEKRKTQLSFASTNQSIQSKQVISLIQQEQEKLQKQQMYECEEIDSIISNKSLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TGG5</accession>
    <name>A0A8S1TGG5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0200112</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TGG5"/>
    <dbReference type="OrthoDB" id="299015at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8150142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="227" mass="25586" checksum="B448163E52339889" modified="2022-10-12" version="1">MNKTNNSIKDLQLRLLISESEKQVALQKLNDIKRSDRAKPSLSFFTPSKDEAKRKDKVGKLLIELMNLIATNNSLQCMLEINSLENMMKMFDQGKCYKLLKKIIKPFTLLITYLLNTNITESLSVRSENQTIQHLNNYNSVQAGIQTPMSQVITINSNNLHNHKTSFPSSIAFKPPSEATTPEKSVKQAPISQPQQKQTIQQPKGIGKSQKPISRRTVTSKLNTCGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TGH1</accession>
    <name>A0A8S1TGH1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0200194</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TGH1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027916">
        <property type="entry name" value="Kinase-like_dom_ROP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14531">
        <property type="entry name" value="Kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="2">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="134"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8150306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="577" mass="68326" checksum="54A974810EFA0FB2" modified="2022-10-12" version="1">MQYQLSIIDIDGFENKWYYKLRITNNNSIYYRDVHVRFQDLNQLQKNLQNESYQSHLPQFPEKSLFYSWFQSKESRNELQETKESVQNYLQGINKNPPFKYEAINHFVQNTYDLKRSQQLKFQSINKDEFYQLNIKKSEIKNGKFNKIFLVKRENQDRVVHQFLMPSNDAAKKDYENYKRAQLLITDYTYIVKCHEIGQIIKKKQQFQRKQKKSIYNELEIGLESSYDIIYAIEDYAKQPINKLIQKRQERQDHFKLETISEALITLIHVAQYLQFLQIFQKQFSVTNLYYDEKIGFKIGGLSPIYIYKKKYRLKNENDPNTYKALNPPELRGSAGGYSIKNNLNSYIKSDIWQIGIVILSMASLTLPINFTQSEAIDNKIKQVELKYGEKLALLLKNMLQRNQNDRFSIDDIIIPAQQLMPMNQMQLKSIKSIERIQILSISQQQLEDMDKQFKEKTSEKQYIINLTIDKHIVQQILIFNFERIKREFVIQLHINVSPQTIPDDLIDKMMSSLVEYQYLQLLVLNLKKCTISDQAQKNIIIAASSIKKLKQLTLDISGNQTIPIPQSKIKVVVYNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TI47</accession>
    <name>A0A8S1TI47_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nuclear transcription factor Y subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0200321</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NFYA/HAP2 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TI47"/>
    <dbReference type="OrthoDB" id="290689at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001289">
        <property type="entry name" value="NFYA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12632">
        <property type="entry name" value="TRANSCRIPTION FACTOR NF-Y ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02045">
        <property type="entry name" value="CBFB_NFYA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00521">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51152">
        <property type="entry name" value="NFYA_HAP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="122"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="122"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="140"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8150549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="150" mass="18399" checksum="87D13D440827F1C8" modified="2022-10-12" version="1">MLQDNQRENEKKQYSMFYPEDERSKSHFLNQQYLTNFFEFTNHYKEPDLQNQGKQEDKNQPKFPIIIQYEQEPRYVNQKQYRRIMIRRIKRAQQALKLEELRTQQATKVLDKSNQKYIYESRHQHALKRERGPDGKFLKKQNSVDSPSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TIP1</accession>
    <name>A0A8S1TIP1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0220094</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8151768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TIP1"/>
    <dbReference type="OrthoDB" id="300881at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8151768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="224" mass="27073" checksum="FE5D5B0B9A9BDC1B" modified="2022-10-12" version="1">MLKKLKLEIKDVTEKFRSNRKYTSLERVKEVEKIVINEIHNKDLDQKELLLHLALEIGHPLKVKSIELKEELENLLQLVLIELINCKESLNKDASEYYQCLEAINDKLYTIIAYKLRQLMKYEQLKFRIPRFGNTQKLLQKKINQLLACEINHSFLQEKRDAEYIDQIRKQYQEKKTIRKLTYGFIFIYFGILFRQKKWFQSINKHYRYALFGFVGAVLFYQLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TSR7</accession>
    <name>A0A8S1TSR7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Macro domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0260045</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8154592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TSR7"/>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11106">
        <property type="entry name" value="GANGLIOSIDE INDUCED DIFFERENTIATION ASSOCIATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11106:SF27">
        <property type="entry name" value="MACRO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro" evidence="2">
        <location>
            <begin position="20"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8154592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="307" mass="35313" checksum="8D855763DE1DC795" modified="2022-10-12" version="1">MGNMCEYHARNINNEQNLQPKLLAQHRFQDIGIYLDAGDIKNEEVDVIVAQTDLNLKLPYYLINNVSPQTKQQLLKYIQQIRDKQAVSHGDVVHSHAAELEFDFVFYCILPNEDDEPMKLARNHNPKNLNLKLPKNAHQKYQKQFIYDCMMTCLKVAEEMEITSICFPVMKSTKFTISTIATMQMIAVKNFLEENVTKIKFLSQVRFCIQDDKDQELFKWAFDKVFMDTDDNSEASDEDSLNNNSGGNSGSGSAGNSLSKNNLYELQPTIKKPQQKNFEEDEFVKSFGANYYDKFESPQSVGRKISI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U1F9</accession>
    <name>A0A8S1U1F9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase C1A papain C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0320314</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8159095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U1F9"/>
    <dbReference type="OrthoDB" id="311597at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000196">
        <property type="entry name" value="Clan CA, family C1, cathepsin B-like cysteine peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0865">Zymogen</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="3">
        <location>
            <begin position="125"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8159095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="339" mass="38510" checksum="8B061AD239783A29" modified="2022-10-12" version="1">MRGSRPLSSQSNQQQVEKMKLAQTAIKKRTSKPNFFTTYRTHVIISIIVGIIIIAVLSFTKQIKEYIIINQSEINSHNSQGYPYTLGPNDFFQNVTLMQAKTLFKNDFTQQINVEKCKVPINFEIPTYFNFKESYPNCSHIIFNQGNCSSSYSIAVSSAFSDRVCKVNQTQQLSAQNLLSCDGKLNQGCTGGHITRSAEYIIKHGLTTNECHPFRGDDNFQECTKALEKCQRYKANSFCQLQNKDDIKRDIINRGPVVAIMQVYKDFLVYRDGVYQVLEGTPRFHGGHAIKIVGWGEQNGYQYWIIENTWGTTWGNEGLAKLAIDGFIDLSLQALSIIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U2E3</accession>
    <name>A0A8S1U2E3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0290183</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U2E3"/>
    <dbReference type="OrthoDB" id="294794at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="254"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8156816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="274" mass="31492" checksum="F50BCDB30791F1AF" modified="2022-10-12" version="1">MGNSNKVQQNVIHIEFMTAKQKIRKDKALIKNKVDQSNNEHLQDIICIQDLTQYYEVDFRQTILTSDETIHVKKIVDLVVNPNSKTPNNLIQISEFFKQNVKSFQIFFNNQMFSQDEQQYQFAYEPLYIEVIYKPKHWMEKAVEKFKTYSNKIMSALGFISKFAYIAIPLYTSLSALQPFLMPTLKTAKVALRSTQNPQTQTAQSVVKFLINTLKRASYIAAPLLSIVGQYMPMSKTMVAGIGLYLGKGMLWNVIKLCITIFCPFVAPLLMIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U2N4</accession>
    <name>A0A8S1U2N4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0300290</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000030">
        <property type="protein sequence ID" value="CAD8157679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U2N4"/>
    <dbReference type="OrthoDB" id="306097at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF98">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="1"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="285"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="365"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="298"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="365"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="390"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8157679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="468" mass="55400" checksum="91C56C72797B8852" modified="2022-10-12" version="1">MGTSSSTFQFNFSQYQYLQEIIDPRFGEIKLYQKNETGELICVIEKIQTQPTKPFKKLEHLNLLKVYYYSASTYKEVCSTFTKLYIVYEYINNNLKTIITNRYKKHQYFTESQLWSLAYQIIDVLNYLKTNGIFPMEMRNLYISNQGYLKFNSLFDNHMTSFQQLFSQFNEDFHISPEELTLFKNQSSINNLNPELCQIFQSGLTILWAATLNDPSTFFNKIEWKLNEQDLYLRISELQYTDNFKNLLIQMLQINPSQRGTLETLLIIISKYKKNDDLLKIQQLDPTQNNHENDEYEYQSQSSYNQSNRKQKLNIQQNKFSQYQKQISLIGSITLKSENYEINRNNQLFNNNKVIKQENNINQQQKSKSFLQFKTPNSSPIQTYRKPPSISASKNQLNSTSKPKIPNQSQFNKDKISDTSYFSQQSYLSNNKSQRSLLENRIEEALMKSKLALAKFDQTIKQNNYYKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U5X0</accession>
    <name>A0A8S1U5X0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ODAD1 central coiled coil region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0350008</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U5X0"/>
    <dbReference type="OrthoDB" id="303056at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051876">
        <property type="entry name" value="ODA-DC/CCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049258">
        <property type="entry name" value="ODAD1_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21694">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21694:SF18">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21773">
        <property type="entry name" value="ODAD1_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ODAD1 central coiled coil region" evidence="4">
        <location>
            <begin position="118"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="222"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="426"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="475"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="132"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="307"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="229"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="434"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="475"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF21773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8160485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="513" mass="60245" checksum="78B6A5E773339136" modified="2022-10-12" version="1">MNQSQTISTLNTSIQKAKSDFQQITTQNRSLISQMKIDNSEYTDQLINQKQKNTVVDTQIALSVIKAQEECALIAQKIEIETKKKQDLENLVNAMKVSAKDISKIENTNSGSELTQVQKAVRDAELKLQQIHIKHNEQLAEVEAEKEQLNLSRRERVIYSTVFKGIEKDAIFKQREYQNCIRQNELLKEEQKFLQDQLKLIKELADKEQNKFQQEYEALFKPQKDDQQEQQSMASDQKSQVQGPYLTEQGDQSKPILKERSESQQEQKDVAQQVLEYEAQFNKLYLETGFNNVDAILKQYYNQEIWIDEIYKEINEINQEIEQTEQKNEKIQQYLQKFKQMAKAPIKEQVNLEDSKIERISRGIDQNKKQIAQIQQQYMRIQEQIGVEIINEDDDETTLLPKIEQLEKIIDNILLVSNQYVPKQDQKKQKKGTVKFDDDKSEISGTKQHTELKSQSDHQFDLDIILTEVEKRKTLAESRHTKEELQKESADQMVLSQNKKQNSKRSKYQQQQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U600</accession>
    <name>A0A8S1U600_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0340141</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL29 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8160078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U600"/>
    <dbReference type="OrthoDB" id="996720at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002673">
        <property type="entry name" value="Ribosomal_eL29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12884">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12884:SF0">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01779">
        <property type="entry name" value="Ribosomal_L29e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="51"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8160078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="73" mass="8489" checksum="D800C7DD943B188D" modified="2022-10-12" version="1">MAKSKNATSHHNARKHHKNGIKKLPNQRYSTLKGCNQRFAKNRRFAIKNDPSIKKNKSLETRLAKRKGNKNIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U836</accession>
    <name>A0A8S1U836_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0350237</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U836"/>
    <dbReference type="OrthoDB" id="288954at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035775458" evidence="1">
        <location>
            <begin position="21"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8160918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="234" mass="26494" checksum="0CF88044482BEA3A" modified="2022-10-12" version="1">MKTLILTVLICFGVSQMLRGSPKSLIEEEQAIFQAEQEDQSFYVGRDDVSAETLEGDQNQEVEASLAVFKSDDTPMVYIEESIDSTQQDGFQQKESLQIEKIQDQMQVEELQEAKTDQANYVDKAISLEGDTEAQPIQNSSTDVVLNIAPKTESLSSSENTKKHVYVYNPLAKKPFVGIKFPPMPVTKDHNYLSNSDTLTIKLPPWSEQSNHRVEDDLEFFQFIRPSDVSVNEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U8I7</accession>
    <name>A0A8S1U8I7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Kelch motif family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0360228</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U8I7"/>
    <dbReference type="OrthoDB" id="191037at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19756">
        <property type="entry name" value="Bbox2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF3">
        <property type="entry name" value="KELCH-LIKE PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="3"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="9">
        <location>
            <begin position="76"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="131"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8161521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="639" mass="74654" checksum="FB0177F258DD5934" modified="2022-10-12" version="1">MECPMCYEFYAPDRIARNLLCGHTYCTICLETMYKVNKRIECPLCRTKHEPNVKPNNLSKNFVAMDLASKHLEEQKTFELCQQHQKYPLQFYCEDDQSNMCTECITEHYGHKFFKYEHSVSLQMNRVGRIQQKLKIQYESLEKQMKKFDNCKEQLELDNIKLIEQIDDQFDRIIAKCEKRRKELKDQLLKIFDSESEQIESELILNQSLLSNMTLLLQKLDEKYNELKTTKAFKGNDFIKEINDLDEQVDKDIGQLKQLKGSELKILPKLNFEQKLINDISKYGKFKKEVSNPQICYFGEKHKILIYNIEKNDWQYRQMSNNTFDYNYYAAAASLPNGDIIITGGGVSRNAMLISPSKGFQQQALKSMYYPRKEHACVYLDGFVYAIGGYDGTTKQMLSCCEKYSLVTDEWKMIDPLQKQKCAFAAATALNKYIYVFGGFDGRDRQNTIERYSVKDNQWKVLELKFKQGFSNAAALSQDDNQILILGGGSNQGFTNSLQVFDPINQTIKIISMMTEGRDLRNKLIIYNNDLYACGGNSNSIEKFQITQQVWTNLKSYNYLIQDNLDSWCSAFTFDVNTSYTVSNFMKNCKQIDLQQNQKYQYQEQIMFENNSFNQDAQSDVSDGQFYSMSNQDQQNDWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U9Z2</accession>
    <name>A0A8S1U9Z2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">cathepsin L</fullName>
            <ecNumber evidence="10">3.4.22.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPENT_87.1.T0350131</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="11">May be involved in extracellular digestion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Specificity close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity.</text>
            <dbReference type="EC" id="3.4.22.15"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.15" evidence="10"/>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U9Z2"/>
    <dbReference type="OrthoDB" id="190265at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000104">
        <property type="entry name" value="Cathepsin L 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035794497" description="cathepsin L" evidence="12">
        <location>
            <begin position="19"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="14">
        <location>
            <begin position="29"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="13">
        <location>
            <begin position="109"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8160713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="307" mass="34464" checksum="9F5595167859337F" modified="2022-10-12" version="1">MQKILSLLTTALLLSGLAFYSQNQESHSFKTWQKKYNKFYTQTEEAYRWIIFNHNVELINKHNSNPNKSYSMAINQFVDLTDEEFQSMYLGKPTFVNIDNIELSTGNNLGEADWSSKMNPIKNQGNCGSCWTFSAIGAVEGFLIIRKGFKGVLSEQQLVDCAVDAGEGCNGGNSDLALDYIAEVGSVYERDYEYTARDGVCKVKQGKVRISGRENYGPNEDAIKKGIQNYPLSVSVDASYWKFYNQGVYDGPCRDDFHNHAVVAVGFDNEGNWKIRNSWGEGWGEQGHIWLKPGNSCAVMTRVDGAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UBA6</accession>
    <name>A0A8S1UBA6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0360160</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UBA6"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000334">
        <property type="entry name" value="Heat shock factor protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF206">
        <property type="entry name" value="HSF-TYPE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="3">
        <location>
            <begin position="4"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8161387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="183" mass="21708" checksum="CCCC360B7AFBF80F" modified="2022-10-12" version="1">MKNQISSFILKTYNILENNMYEDIVSWNEDGLSFTVKNISQFSSIVLPIHFKHQNFSSFIRQLNMYDFHKTRGGSVNEFKNEYFQKGRKDLLHQIKRKAHNELIPINQPIQNDSQDQLNQLSQKCDYLHNLCSTLLERNHRVIKDNKTLQKVLTSNNKLYLQDEFKQPEQLCITNEELTNNVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UBV3</accession>
    <name>A0A8S1UBV3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0370310</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8162318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UBV3"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16454">
        <property type="entry name" value="RING-H2_PA-TM-RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:001411">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46539">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ATL42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46539:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ATL42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="11">
        <location>
            <begin position="90"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="314"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="94"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="111"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8162318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="408" mass="48165" checksum="E5295B3DC99228E3" modified="2022-10-12" version="1">MKQGYLFFLVGVQCILYQFNATNQYLLVENEVEEWKQMKIQIYEHNVKQIEAIENYEKYLQLYYKLKLGSYYELEIVIIPESNFKIQQINQKECPKQCSNNGICINTSCQCYELFFGSRCQFEIESYYNIQQYNIELHQFETSYLLLQYQQSDLINEQGQTKNMILQSNHRIEIQLYVVLPYQNISTSIQNQNILYNQSLNGSTTIDFSSFYQSYSSSSISYSFFFIFTNNYTNNQRYSITFELKSQEITFQDFILVTILSVVAFIILLIVIFIVRRRCQYIQISKLKYSIDNQPKIDIVGQLPIVTPAKDEECIVCLDLLENQQCRQTSCKHIFHEQCLNEWLQKQQTCPLCRDNLFEEEGAQIMKIRNLSSSSIVAGQFSIIQSERQPIQQIILSRSMNNRLAAFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UEZ9</accession>
    <name>A0A8S1UEZ9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0370282</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8162262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UEZ9"/>
    <dbReference type="OrthoDB" id="289147at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="249"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="309"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="333"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8162262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="435" mass="51336" checksum="CD99F998B1BA3E28" modified="2022-10-12" version="1">MELEKTLHRVQERILTHQCAPQIMNICSKILLSMVSINLLIIWGLSNRTINQISFDQETKDNIYHFSIIDEDNTMLMMKYAKTQELLHLKTELLQSHNFTIINISIDYNNYFDSNLQKLLSLTTNLETLFLHDIAYSIQSDIYVKNNATNQTYIWKEKRAPQNQLGKIIQHLWEFSIITFGLFISSAISSLYIKITIICAPVIIIIMLEVSYLFGNRQIFPIFLARAFPWIGLYLNILDRTQRSKKQLIIAFALMLFLIYFIYLSSVIIGGFLLFKSQVPFSLEDNFFGLVTVNEFASLLFLRTRSSLYFVPKFTIIYYYLFLWYVQSTNYGFYSLAMLTLSYVCLGTFCLFIYLYEIPSLGWNPLSYYTPTIDRPRCYYLPVFSLNWVNDLPQLWSMFYPLHGRRYFQIQNLALVDRNFPLLNNLLDIEMQEQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UFN1</accession>
    <name>A0A8S1UFN1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0390287</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UFN1"/>
    <dbReference type="OrthoDB" id="10327969at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8163570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="200" mass="23681" checksum="AC5B807C7DA11C01" modified="2022-10-12" version="1">MSNSFSQEIQTSYQKTITTSQNVQRTTDVDFQDAVQRYELRRSSKPKKQIINQKTGIVGNQYMQISTDDNINNYNFQSSKHQQRIVQKYSNINSANEVNKYNVQEVKDEYERGKSLGQLSKTEKYNFAGPLTEYEEEFTQQKENVLNYNGIQTQQITYQIDYEQIKEDCQYKSQQYKSPQREDDEQIQLLDTDYVSCKIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UFU9</accession>
    <name>A0A8S1UFU9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fungal lipase-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0380326</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UFU9"/>
    <dbReference type="OrthoDB" id="424277at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051218">
        <property type="entry name" value="Sec_MonoDiacylglyc_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45856">
        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45856:SF25">
        <property type="entry name" value="FUNGAL LIPASE-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035835675" description="Fungal lipase-type domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Fungal lipase-type" evidence="2">
        <location>
            <begin position="87"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8162953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="289" mass="33179" checksum="4EF407826B996F8D" modified="2022-10-12" version="1">MKKILLISLVIVLSYQTVEYNTYDEAFAKHLFYLTSASYCGEQHIRNWNCGKPCKELKPITDITFFINATNENAGYGAYHPETDEIYLVFRGTLPWSLTNWVEDIDFIKTDYPYCPNNCQVHRGFYYSFLGIQDQVLNSLKSLTKKYPLAKITITGHSLGGALANHALVYLTTRGFTISKFYTFGSPRVGDKNFFTYVNQQLFPGPKYRITHNHDPVPHLPALIQGFHHVNQEAYYKDYLLVIHKKVQLCNDNLKEDDSCSNSNVADLAVSDHTNYMGYDMTLELLTCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UHW7</accession>
    <name>A0A8S1UHW7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">alpha-amylase</fullName>
            <ecNumber evidence="4">3.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0400149</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endohydrolysis of (1-&gt;4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1-&gt;4)-alpha-linked D-glucose units.</text>
            <dbReference type="EC" id="3.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.1" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8163954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UHW7"/>
    <dbReference type="OrthoDB" id="1740265at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11319">
        <property type="entry name" value="AmyAc_euk_AmyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013777">
        <property type="entry name" value="A-amylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357:SF215">
        <property type="entry name" value="ALPHA-AMYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357">
        <property type="entry name" value="ALPHA-AMYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001024">
        <property type="entry name" value="Alph-amyl_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035741654" description="alpha-amylase" evidence="11">
        <location>
            <begin position="19"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="12">
        <location>
            <begin position="29"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8163954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="480" mass="55615" checksum="5317A395DB944D72" modified="2022-10-12" version="1">MKLLLSLFVLVVLIHCKSKDEWKSRSVYQLLTDRFATSQGRSTSCNLGNYCGGDYQGMIQQLDYIQNLGFDAIWITPVIDNYDGGYHGYWARNMYDVNHNFGSADDLKALVNACHARDIWVMVDVVANHMGNTNTVYNQNNPFNQSSHYHDWCDITDNDFNSHNLYNIERCRLAGLADLNQDNQFVTEQLLQWIKWLVQEFKFDGLRIDTLMMVKTDFWYKFSNAAGVYTVGEVFDGDMNFLKQFVGPVDALLNYPLFYTARDVFLHWRDMNTFENYYNSLMNSWGKQNIQYAGNFNDNHDNARFLNDQIQGYIPDEVFLSDKPHLTFSALKKLQFKAITAFCLTSIGIPMIYYGSEQLYAGGNDPQNREVLWGNLDQQSEMYKYIQAINNARKATNAGNQEQIQRYSDSEIYAFTRGTLFAAFSSKYDRQTIKTITYHPYAEGTTLCNIFYPTTDCIKITNGKFTLYLNYGEVKIFIPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UJU0</accession>
    <name>A0A8S1UJU0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Small nuclear ribonucleoprotein E</fullName>
            <shortName evidence="11">snRNP-E</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Sm protein E</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0420037</name>
        <name evidence="14" type="ORF">PPENT_87.1.T1420112</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000142">
        <property type="protein sequence ID" value="CAD8206000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UJU0"/>
    <dbReference type="OrthoDB" id="25620at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01718">
        <property type="entry name" value="Sm_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027078">
        <property type="entry name" value="snRNP-E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11193">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8 11" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="12">
        <location>
            <begin position="18"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8165090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8206000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="95" mass="11179" checksum="C53A2408371E0A18" modified="2022-10-12" version="1">MQHPPYQRKPQKKITQPLHAFFTLFTEKTQLEIWLVENDNLRIQGKLAGFDEYLNLVLDRAVEYDRKTKATKELGKILLKGENIVLVRTLEDVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UK27</accession>
    <name>A0A8S1UK27_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">G-protein coupled receptors family 2 profile 2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0410071</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UK27"/>
    <dbReference type="OrthoDB" id="309073at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="265"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8164513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="330" mass="39540" checksum="57413ED986294053" modified="2022-10-12" version="1">MFQCQNQKLEEVYQEHCEQNLNSTDKIVTFILRIIILIISILSSLLVLLIIRKSNKTQFWPFKLIKMQVISELIDLTFALLFTINSSCWPKVCKFIAYIMHSNWLASLNFMLFQCFIYFCLIKSELLFNYIMNYLQYFIIFLYIVPYGILFFVLYDDGFGPSGWYLQNGEFNFIFCGFINNQLIGFWITPVSIIFILSIMLSVFNSYYWYHLKNKKGLKYPALRIQSIIIFPLLYSFAWIINFLIRLFDTDAKNQNYCPIKEMNWVFYIFYLILNLGFELHLTLGAILFYFIYRGLFKIDSLWDDICFRKRQKITSNQLYLTETNSNIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UKA2</accession>
    <name>A0A8S1UKA2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0420264</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UKA2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8165528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="184" mass="22120" checksum="7CE6EBCADB7D568F" modified="2022-10-12" version="1">MYAEKYIYKLPKMNLRTIQILVNLIKLIQLMSTRKLLLILLKLFFWPNLKLTQQIILKLFLLMKIPKSLLEKVQSKQSNILTYEYDWNKALKYGNLDKMIIALQFYVIKPQHFNTQIISENHCCFLINFNKGKSYLFRKNDRCQSKYLGFCFECRNIQKNVQLRLDNILLMGQLDSLDYKNQSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UKP3</accession>
    <name>A0A8S1UKP3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0400343</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8164342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="300625at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="270"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="124"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="270"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8164342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="364" mass="41494" checksum="722E0BC131535DA3" modified="2022-10-12" version="1">MDQQNDVVTQGQSQELNEQQDETSTQNEPIEQTIAENPPITETIPMIDPSIDQNKDQPTNQDNKTEQSNDPPASNPIYTYSILAKEFILFSLPYLYFIFAALYRIYQLQXDYASAEITDSTTEIATLTSKVEQLAQDVDNKKIQLDILNEQETQLTEQRANDADDFIKFEHETENVIEAIEVIFTKLSSIQPEQEVVQILTQLNQIGASNPILALMQVASTFSKDQLNNVLNKLSEVSASLEQSLHDARQSEIQAQLDYERLMVEIESQRESLSSAREDSERQLKDNEQALDLQKKRKEDATDELNAATSGKEQKEGECDGWRTQYAADTEHRQQEISIIRQIEEILATKLKNVKVYLLERSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UM17</accession>
    <name>A0A8S1UM17_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0420216</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UM17"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8165444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="152" mass="18009" checksum="742787FD33BC63F5" modified="2022-10-12" version="1">MHQDLKLRQIIKPIFQTILKIYNGRYYENIELYENQRSFPQQLNGCSLPNGKKIKIISAVQIQIYIRRSSANERSEKTGDLKADRVRYTEPSLLQVIQRFEKYIELVLCFCLIRNIQNHQITVVCWNISSLKPTTLQIIKENDPHLIYLQET</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UMB4</accession>
    <name>A0A8S1UMB4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0440133</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNAP-25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UMB4"/>
    <dbReference type="OrthoDB" id="309919at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305">
        <property type="entry name" value="SYNAPTOSOMAL ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305:SF9">
        <property type="entry name" value="SYNAPTOSOMAL-ASSOCIATED PROTEIN 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="4">
        <location>
            <begin position="47"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="4">
        <location>
            <begin position="111"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="3"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="139"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="104"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8166408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="175" mass="20908" checksum="4A42EABD95FDA657" modified="2022-10-12" version="1">MQQQQIQKQNQAALNQEQELKLKQQQQDILNNMYQINQQSANSLLTLHNQGEKIDRIEQNLDQNQQKLHKIDYILDVIKSPWGQFKALFTKPKQEKLEQKEKNNQQQQIQQPPQRQVDEIYDEMINQTNQMKYQQLLTNQVLKNQNTQLDKINQKADNQTVQMAKQNEKTKKIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UN50</accession>
    <name>A0A8S1UN50_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosomal protein eL8/eL30/eS12/Gadd45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0430155</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8165844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UN50"/>
    <dbReference type="OrthoDB" id="308597at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000003">
        <property type="entry name" value="60S ribosomal protein L7a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="5">
        <location>
            <begin position="121"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8165844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="259" mass="29922" checksum="54FE2FE53966E488" modified="2022-10-12" version="1">MPKAPKTSKAKKVQKKVADRKKNPLFVRDAKNFRIGNDVQPKRDLSRYVRWPRYILLHRQKKILLQRIKVPAAIHQFSKTLDKNQSSKVYSLLKKYAPETKTEKKQRLVKAAESKAQNQKTDSKKVTVLKFGLNHVTTLVETKKAKLVLIAYDVDPIELVVWLPQLCRRQEVPFAFVKNKARLGALVHQKTATCVALTEVRKEDQAEFDNLARDLRQHYNENHELLRTIGGGQVGIKSKHQQEALKKAFELEELKKTSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UQJ9</accession>
    <name>A0A8S1UQJ9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0430259</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Fe(II)-heme o + 2 A + H2O = Fe(II)-heme a + 2 AH2</text>
            <dbReference type="Rhea" id="RHEA:63388"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:60530"/>
            <dbReference type="ChEBI" id="CHEBI:61715"/>
            <dbReference type="EC" id="1.17.99.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:63389"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Porphyrin-containing compound metabolism; heme A biosynthesis; heme A from heme O: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8166052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UQJ9"/>
    <dbReference type="OrthoDB" id="1726137at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120547">
        <property type="term" value="F:heme A synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016653">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006784">
        <property type="term" value="P:heme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003780">
        <property type="entry name" value="COX15/CtaA_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023754">
        <property type="entry name" value="HemeA_Synthase_type2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23289">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23289:SF2">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02628">
        <property type="entry name" value="COX15-CtaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="210"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="322"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="350"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="381"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8166052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="413" mass="47368" checksum="607DA6858B6A6F52" modified="2022-10-12" version="1">MLLSRIRTFKNLTTTLKPLQNNRYYKFSNAEASKNAKFDRNAHFEDPVNDLVDPSKRWLLGGWLLLCAGGVYFMVLLGGYTRLTHSGLSMTKWKPIEYIYPRNQADWEKEFDHYKQFPEYQHTPIDLDKFKKIFIVEYLHRVSGSTLGAIYVLPLAAFTAMKWIKPQYAKRLGAIGGLGLLQGLIGWWMVKSGLDRPKPEYQQKPRVSTYRLTVHLSMALSLYSLLIWNAATLFRKPQHLQITPENYKTHLKFRGLGIGLIHLVAINLLTGAAMAGIDAGKVFNTWPTMNGQIIPANLFKTSPFYKNFFENVVMVQFNHRNAAYLTYGFGSYITYLAYKSNLPKQARYICYALYGVLNYQLLTGIVALLNQVQVHSGSIHQFNALNVLTASLLLCHSVRRPSVPYRNYLKQFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UYP7</accession>
    <name>A0A8S1UYP7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 11">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="2 11">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0500107</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="11">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="2 11"/>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8169012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UYP7"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07834">
        <property type="entry name" value="STKc_MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000028">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000098">
        <property type="entry name" value="Mitogen-activated protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="35"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8169012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="368" mass="42464" checksum="4D315B694D070306" modified="2022-10-12" version="1">MRQNDKHPIKSKIPVAKGKHAFECGGQTFVVDEKYEFIKQIGQGAYGVVCSAKNKKNGQMVAVKKIPNAFEDLVDAKRIVREIKLLKFFDHENIISLVDLPRPESKTGFNDIYIITDLMGTDLHKVIYSSQALTDEHIQYFAYQMLRGLLYIHTANVIHRDLKPSNILLNKDCDLKICDLGLARGYETEEEFKTEYVITRWYRAPEVILNASEYTKAVDIYAAGCIIAELLGRTPLFPGEDYLDQVQRIISVLGTPTPDDMKYIGNPNAINYIKSLPKRTKQSFTQLYPKANPKVCELLTKMITFNPDKRYTVEQCLEHDYFDGLHNPEAEPRCDKVFDWSWDNFELKRETLQKMVYEESLQFNPVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V349</accession>
    <name>A0A8S1V349_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0520183</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000052">
        <property type="protein sequence ID" value="CAD8170159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V349"/>
    <dbReference type="OrthoDB" id="296491at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05578">
        <property type="entry name" value="STKc_Yank1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000454">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000574">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF374">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="289"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="10">
        <location>
            <begin position="548"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="223"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="622"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="224"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="257"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="622"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="318"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8170159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="641" mass="74263" checksum="1A306CEDB8383472" modified="2022-10-12" version="1">MSQQNAKNLRDLLIKQQLNSKIKFKGEPVNKIQPNRKQSGNWDKKVDITQCCCNQKNCNICVKKRVFINASPLLKVKVCTKNQVCEPDSKREFKVTSSVSAHQSPNREKQYQMQQTFLQKQIQNHLQQLQEARPQTRQIPKEQVNKPQFYQNMDNFLNNYYQKSNLSSSQLYQNSFSVKSKVIPKRIESKSHVLSNTQTSFKLKLNQQGPKFSAIINSGLIESAPSSPNNHENSSKSKITPNDSITKSKNNNQNDSKLSDIRRKSTGEISEKTQVLSQKIDEKISRMHFKFLYVIGKGGFGRVWRVEMKRNKKLYALKEMQKAKVIQKRSVNSVLNEKFLLENLHHPFLVNMSYAFQDRENLFLIIDLLTGGDLRFHLGKMRRFSEAQTKFFVACILLALTYLHQNGIIHRDLKPENLVLDEDGYMRLTDLGIARMNKGNNAGDTSGTPGYMAPEVMCRMDHSIVVDYYALGVITYELMLGRRPYNGRTRQDIREQILAKQVQVKREELPLGWSESSLDFVNQLILRKPEKRLGSNGIEEILHHSWLRGFPWDELLKKKVQALYVPGAVDDNFDFQNQISEETQLNDEQILENQGLLRRDTVQSLFDGYNFDVEIKNIQHQQTQKNYTQRKSQPVSPEKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V9L5</accession>
    <name>A0A8S1V9L5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RanBP2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0570115</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V9L5"/>
    <dbReference type="OrthoDB" id="448399at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="4">
        <location>
            <begin position="8"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8172482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="128" mass="14788" checksum="C9D0ABC14D55EF5A" modified="2022-10-12" version="1">MQNKPCDWICGECRNLNYSFRKVCNRCQQCTRDAPGTRFIPNKVDQGLQILQTIKLQEQDLGGSNHSSVETNDDEDVVFSQALFLEDLSKKTENLNKSFSFLKKCSICQCQNYFYQQKCSQCGFSQFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VAE8</accession>
    <name>A0A8S1VAE8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0590182</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000059">
        <property type="protein sequence ID" value="CAD8173551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VAE8"/>
    <dbReference type="OrthoDB" id="313253at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8173551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="155" mass="18314" checksum="70FF6B0D629102A9" modified="2022-10-12" version="1">MIDRFWGHKTQRLPQPIYEEEKQVQIQQEIIPQEVKKEILPLIKQKTEPQLKKIMSSKQFKESLLFDERTSSIPKTTIQNHIIEDLIRDSDIFLKKPEIIKQVQSLKRLKVLKKYEANHSNLPGVGKLPYIINDYHSKSTNPGYSRNNKGNFFTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VC00</accession>
    <name>A0A8S1VC00_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0610101</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VC00"/>
    <dbReference type="OrthoDB" id="504170at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF30">
        <property type="entry name" value="MATERNAL EMBRYONIC LEUCINE ZIPPER KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="91"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8174454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="409" mass="47270" checksum="7A57EE9C0A2AB5A4" modified="2022-10-12" version="1">MKTQFDKSRIVNVYSPNLPPFKLTLTFKTTFNDVRNQIIQGWSIIPKRIRIFNQNGHELKEQDLQFVLDGNKLYITLFGQDLDASIIFQEYEIVSEIGKGGQGTVLLAKHKFIGSFVAVKITRDTEEEDAIIKRESKILKDLQHRNIVKVFQTLYFSKTKESIIIMEYLDGGSLLQLIRNKGTIPEEEVKIYIKQIVEALLFCHSKTFIHRDLKLENIMLVNQGNTLVKLIDFGLAGVAGTQRETETMNVGTLPYMPPEVLNGKLKFVCPCVDVWGLGVIMYSLLYGKLPFKGRTNEEKINNILQCNFQHELNVSDDAKDLINRIFVPKHNQRISMQEILAHKWIGGNGFLQFRRLLKKSENPYLKNVKRKYDFEIRKKSVCDEQKVRISLPLIRYRASSQDKIIDISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VF36</accession>
    <name>A0A8S1VF36_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0630095</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VF36"/>
    <dbReference type="OrthoDB" id="307338at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8175517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="302" mass="35647" checksum="36D4DEEBCDC03B30" modified="2022-10-12" version="1">MSSEFLDYTKNKRKENLDFLSQFMKNHQINVETIVSPQQSIVTSQMTDQVIRIVNSKQSLQTQQSPTSITPLQSPKLVDTTPIIQEIDSILEKIQMKTYNPQKQTPEKKQSPVKIEQPKQQDIIQQIKMKIPQNQSKKQKEEILIQNQKQETQITEPEENIIIQSKEPSPKKESSIKDEVKRIRDTFKPPKSQKIEKPIDQEFEEPKQELITTSQFPQFINEDSSMEYVTPIKKMEIYSQLSYSQSNSILQSELKETTQKPEFNLMKDPVIEELIQEELILMQRLHFLQQQLQYEQRQHEFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VQA5</accession>
    <name>A0A8S1VQA5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0680101</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000068">
        <property type="protein sequence ID" value="CAD8177812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VQA5"/>
    <dbReference type="OrthoDB" id="289622at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8177812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="394" mass="45628" checksum="B7C8F4618B626161" modified="2022-10-12" version="1">MLKSILRVSYKIGMNSQLQQLPVYSSAIQALFQNEYAQAQEYLFQLKQQVENLQDPNASQEVLDLFVQLYKQTKNYKDLISIQEEQFFTYLSMNSIYNEDNSESLKELFRSYIEFDPKRGQAMLSFLQEKVDEIIPLGFQKEFYTYAGTLELLNKNYENSQILLTGAQDSGAKGSLAGMCLNNQVILSELNLEGEIQKLKGDFDAFCQQNIKNLLDAIISFELTSVEENKKELYYLYKLIEQTESYIDKKEINAIINGTQIVNPNSYSVIFNLIDLLINHQCPDKATAWIKVVFRNKTQNPNLNIMNFGRSLLQYAKISMIDNKINQAETVLLDFLNSQQPRLSFLQMMGLTQYSLLLKQENRQYEADQYEKQALNISSKISPTSYIREQIILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VTQ1</accession>
    <name>A0A8S1VTQ1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0760007</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000076">
        <property type="protein sequence ID" value="CAD8181208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VTQ1"/>
    <dbReference type="OrthoDB" id="304185at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8181208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="243" mass="28849" checksum="EF5BD9C303C6E0E9" modified="2022-10-12" version="1">MFINLFFEMFQKNSSKVIFQFSSNKVNLKISDKRKILEAEMKSKVQTLKFQTKKLFQTLISNLNANDIKLETIEFLGSLLHDYEFPISNFLYLFEINSQLFSFWLIKFLFSELYQSLGLNFQIELKKELFEQNCFIVGSLMYQIIMDLLKSQWFSKLGNQSNLLSKYQTYPRPLGPLLSDNYQQPKDVAEVRQIIHSHDIILQTYLLNLLLLILNRQTSSVMIIMFHYLFLFKARFGLIQIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VTZ4</accession>
    <name>A0A8S1VTZ4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0710157</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000071">
        <property type="protein sequence ID" value="CAD8179259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VTZ4"/>
    <dbReference type="OrthoDB" id="10415682at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8179259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="166" mass="19716" checksum="BF187164F89E1F2C" modified="2022-10-12" version="1">MNQNNDAKIKCSNPEHFDDVKFACFNESCKADRLYFFQCIKNGTHISHPQNQQELPFLFEHIQRIEKQCEDLIKNLKKIIDAAHQQFNLLIEGIRSKYKKSKQEFLTLNSKQMNSFFTQSIQFNSFEKIILLQTQQSLKDFQDSIEKSLSDLKLYFHLVLLQNNYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VV59</accession>
    <name>A0A8S1VV59_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0760032</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000076">
        <property type="protein sequence ID" value="CAD8181250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VV59"/>
    <dbReference type="OrthoDB" id="364348at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="251"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="3">
        <location>
            <begin position="178"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="19"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8181250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="271" mass="32099" checksum="C5CB0B377FE00EF6" modified="2022-10-12" version="1">MSDLHTSLLNNDMNFDQLYEIVRQELVEFQKNVDELQVLGESEIDQQNVNQVFQALKTIQLQQLNIKEKIIKLEQQSRGVEQRNKTKKLRDLYTSHLQRQQQLYKNMINDCGYEPLKDVLKEIDKMMKLSSKQDVQNYTLNSQIQSKQSTIETNRTQSQMQLLEIEPLQQSEHLDWNNQILKQNQEDIDQIQIKTQTINKIVQDLALEVEHQDTFFDIIETNVNKTKEIVIKTQDELEKTQEQQKSGKKKLWCMLICAFITFLVFLLILLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VWW6</accession>
    <name>A0A8S1VWW6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tubby C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0770098</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000077">
        <property type="protein sequence ID" value="CAD8181840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VWW6"/>
    <dbReference type="OrthoDB" id="8775810at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000007">
        <property type="entry name" value="Tubby_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517:SF7">
        <property type="entry name" value="PROTEIN KING TUBBY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517">
        <property type="entry name" value="TUBBY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01167">
        <property type="entry name" value="Tub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubby C-terminal" evidence="2">
        <location>
            <begin position="115"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8181840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="336" mass="39281" checksum="F710E9366D4488F2" modified="2022-10-12" version="1">MNQNQDDNSSDDVIQFEDNEIIDFYNMEQKTSPKDNKIQQQEHNIAYELKNSSQLMLIQEQSHPKQQQMQQSQKSPIPIKQHQKSDSPELQKFKDNNYTILQNLQIDFSLEQFLISPAPKGGMIQCRFYRASPKFTLFLEQFNNILLNAEKKSFQIKGSYKIYAKGKKEQYLGKIKGDFMGLKYLLYDNGKTAKKSKDINQLRKELGVIYYQPYEKKLRSIQTFIPQITSSGQLYEFKNQKPYTSIINNLDNVIELINRKPQWISSYKAYGLNFYGRIFTSSVKNFILTERGSEIPVMLFGKNDDREFVLDFSYPLTPVQAFCIALSSMDHKFGCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W369</accession>
    <name>A0A8S1W369_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0810118</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M67A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000081">
        <property type="protein sequence ID" value="CAD8183671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W369"/>
    <dbReference type="OrthoDB" id="10256771at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005838">
        <property type="term" value="C:proteasome regulatory particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08062">
        <property type="entry name" value="MPN_RPN7_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000040">
        <property type="entry name" value="26S proteasome regulatory subunit RPN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024969">
        <property type="entry name" value="EIF3F/CSN6-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033858">
        <property type="entry name" value="MPN_RPN7_8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540:SF7">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13012">
        <property type="entry name" value="MitMem_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MPN" evidence="3">
        <location>
            <begin position="10"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8183671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="289" mass="33072" checksum="C93AEC2D6E238AE2" modified="2022-10-12" version="1">MSNMNKPPLVIIHPLVLISVVDHYNRIISKTQQPRVVGALLGERKADGVIDITNSYALPFEEDPKDQNIWYLDHIYNETLFELHRKININEKIVGWYSTGSRFKPNDIQINQIFYKYTTTPIFVIIDVHQFDPLSLPTEAYTSVDEISKSGEIVQNFVHIPSTVQAFEPEEIGVEQLLREINNVDTQSLSAKAEQKINGVKGMNKKIAQIQQYLNLIQQGKVKPNQLIINNLQEILNYLPNLGSQDVVQAFTTKNNDNMLTIYLASLMRSIIAYHNLINNQAQQEKKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W5U2</accession>
    <name>A0A8S1W5U2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0810166</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000081">
        <property type="protein sequence ID" value="CAD8183765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W5U2"/>
    <dbReference type="OrthoDB" id="346907at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000654">
        <property type="entry name" value="Protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="14"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="270"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8183765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="587" mass="69234" checksum="4E76E560FDB0D1A4" modified="2022-10-12" version="1">MAEEWITKQIDKYIIVNQKLGSGAFGTVYRGFKKNDETKQVAVKAISIASIKDSAKMVEHIKREISILQQANNPHIVKLYDVARTPHYLYLFLEYCHDGDLKKYLSTKYGRRLSEVEAVIFLKHLVEGFRTLHQLKIIHRDIKPANILLHKGVAKITDFGFARVIDTGMNDPAYFSRVGSPLYMAPQILEGQPFSSKCDVWSMGVMLFEMLYGKPPWDGDNQYNLLQNIKKTVLMIPDAPVRSDKIKQLLRHMLVVQEKDRYSWEQIFNHEIIQIQEAQIKNNLEQLMKEKDELSRSESLNKLYLEMNLVVGYLDQPEQILQEPSTPQLTQGSEEKQSVDDINHEKGLKIINQYETEQKRRKAMLKYNTYFLFERNIAFFFNYVIQKIIKMSHQGILKLTQELYYSTIFCISKNQNVHLKRMYDQLMSTNPDKFDRETWGRYLISQEYKKILTVTKNDIKHTEDFYLEIYKKEKQIIDKELTQPDNKRATKIKQVLDVNFDQNNFFQQLYQSVVQEGLDVIKTSIKQTKDSDPVYKDLLQLGWFLVICLNPYLEFKDINMDFNQFYEEMQTLTETQFLEKIGKRLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W7C8</accession>
    <name>A0A8S1W7C8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0830099</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000083">
        <property type="protein sequence ID" value="CAD8184512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="431588at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028089">
        <property type="entry name" value="DUF4455"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027914">
        <property type="entry name" value="DUF4456"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21444">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21444:SF14">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14643">
        <property type="entry name" value="DUF4455"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14644">
        <property type="entry name" value="DUF4456"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4455" evidence="3">
        <location>
            <begin position="101"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4456" evidence="4">
        <location>
            <begin position="1142"/>
            <end position="1277"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4456" evidence="4">
        <location>
            <begin position="1503"/>
            <end position="1703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="602"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="677"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="781"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1752"/>
            <end position="1778"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="465"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="784"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1752"/>
            <end position="1763"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8184512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1842" mass="219397" checksum="F890EB8D16A87110" modified="2022-10-12" version="1">MKSTFTRSAQRSLEEQPISSQFKASSQLHAALLNEYKRPENSSMPKVTLTSTGKEAVRQARLEKEVQKFEYTLQSEVASLPKHPAPIYREQTKLLIERQKDTRRENHLKFCDEFEKWVNRLNKNLEEAYKDIKRDITDFFSESDQQITNYYQTLTDEALLKREIEFVESIHSTVNAHREKRETKVNTLDKRLEDLEKERFRSLELFCNRFQEGLIDVAFQLEPEITAHVDTFREEFRKVVAEKRADNQVYVQEVNRQHQETFKQYDETQIQKEQRWRDLKHQHYIEEFNVDIQKLEYVYPDERTQLYDKFRKTQKDIFRERKQLLEKLNSYDLAALTKTIVEKWIDETKSYNELASQTIDVCCNNLLEETKKTHNYCMQRYELIKSQLLYARAKSEEELNQLLEEQFLPQVNGLNQSARDLMKQAIDYYERIDQQQTDILTNYGQFFMKIATKNDEYKTEMQLLMHNYEIQKAQAADKNDEVLEELTKKLKEDKQKLTEALHHPRLEECLTQCYKDLDQFGEEYERFHQDNQSIANQHPNIIKNKHQIFEKQVLNIFELVDPQKEQFLQEKYRRIAETKIKYLIAKEEYNKKIEEEKLIEEAKNKKGGKPPATKPKDPKKFQQELEDRMKQLLKEQPPQEVSKYHSSLDQQFVLFRSTNEIAERLYIPPDEMEQVLAKEKQEADEIESYLQQQNAPKDPKAKPTAPPPIELPNYIPEEDVPKKSVDFAEQPPINVLNQAVLEKDAIITIEYLSTLLNQTKQKLFDYITSCLNFQNQQANESDNEFIKQSENQRNEKQSQIEPQKQEIKNTIYILRSQQITMHKKRYERYAKQLVERIDDQTEQINFLLEGGLLDMKDYIQEQQTLKNNLQSATTLAKLQGIQNTVKENYFKFGEKINDLEQKLSYLANQELDVLLQKNKDFMRDCKPNEYFKEELEWYQQMMDEHNATIVKHKEKRTQRLEEIKKVLTAKKQEQLDKFEQEYIIAVEDLAAKDGTGKKYGRPKRIAQEKLRTEMTKCEKAQECINLKIQELKDHYDLFKKKQGDYFANLEPSFSIKIRKILSGTLICIKRYANHIEALKPDNKVVDMPRTTWKENKFDTILEVEEQQEDKKIIENDLESMGPLFYAEKKLQESLLDIEKIIKEEAIKIYADKQKFLTGTDKIPDYLRAYIENMQRNMNEFRIQSIRELRNACEELSEMSPKISEMILLSNHQRWIFTLSLINTNLWNELQKEQNKQENLKKLHQKLLRPNLGNPQLKEELEDLKQKESXQELDVLLQKNKDFMRDCKPNEYFKEELEWYQQMMDEHNATIVKHKEKRTQRLEEIKKVLTAKKQEQLDKFEQEYIIAVEDLAAKDGTGKKYGRPKRIAQEKLRTEMTKCEKAQECINLKIQELKDHYDLFKNKQGDYFANLEPSFSIKIRKILSGTLICIKRYANHIEALKPDNKVVDMPRTTWKENKFDTILEVEEQQEDKKIIENDLESMGPLFYAEKKLQESLLDIEKIIKEEAIKIYADKQKFLTGTDKIPDYLRAYIENMQRNMNEFRIQSIRELRNACEELSEMSPKISEMILLSNHQRWIFTLSLINTNLWNELQKEQNKQENLKKLHQKLLRPNLGNPQLKEELDDLKQKESKRLSDFLELLSKYQVTLFDENFNQGDKYLKAANQNFEFLLLFYDSLLLFEDFIKLPGDEQIQKKHQNLKVSLRQKQSGQIIDTNSERSLSKTWEGVQLEMFTIGDRIIQYNWQINQVVKVDPKDAKNKAPEAKKPAPGGKQQEIEKPIQTTTTPLKSFKTFRQISAKHEFFKSINEFVIEYKKQVDYAKDQIKLLSERELKFQFKWEQSIASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W7Z8</accession>
    <name>A0A8S1W7Z8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0850111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W7Z8"/>
    <dbReference type="OrthoDB" id="303958at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8185491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="298" mass="34819" checksum="E5BA32AAC303EA99" modified="2022-10-12" version="1">MNISAVRSIFSLLSLNALLQTYENQPVEFWDSNQMMSHPRLTPIFQSNQYINLLAGLATLILIDKKQKLLMTNSILTGTSVIIQDLNLFLPKTFNMITIYLSLIALIAQLFQLLSFYYDNIQQTSQIIKQDSFKEQKQIKKQNQRKMVLSKKKEKPLQKLQKSQQTTQIEVIEQVQYDKSEVESIENDFNEFLKPQSNDQTITEMETENLYEINHADLSQISQTKEDPKLTFQLKFNGEVSTLNCTFDTFDSTMSILNSTYTLKQIKQIKIHLLLNLYDENEDYTCRLWCLKKLLILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WDE4</accession>
    <name>A0A8S1WDE4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0860183</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000086">
        <property type="protein sequence ID" value="CAD8186149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WDE4"/>
    <dbReference type="OrthoDB" id="308390at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="300"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8186149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="361" mass="42582" checksum="E6E414936AFA5D1D" modified="2022-10-12" version="1">MKSFRSEEPLSTTSKNKGFTFEIGNQSTTRKQQKIPFQFNSLNTEECDNDTHRSQQAFKKLKNLEFQNDLFMLEMQHGQRRKEAESNRLKFQLSSIKSDIDFMKSQLQSPIAKVNTKRKRSQSIDSEQSSDRSSSSSSDNSYVKCPIKFKHYEPIQKDQIKIDSFETIENRFKQLFQISDVNDKLNWMIRQYFNSYHSIQLDLQNNLLPLLLDNTLKLLIEIIEKLNSIKLDTLQFKSYDPFSNGDKNNKRYSSDLDIETINQEYQFQVNHTQTDYCQKFNEQPQKNQFSTPQYKQQQQQQQVEQKQSARQLSNMMKSKPDSSCQTSNNQTLDKKVSISERSQKKTTQKTQLMPSLKNRQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WJ02</accession>
    <name>A0A8S1WJ02_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0920018</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000092">
        <property type="protein sequence ID" value="CAD8188647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WJ02"/>
    <dbReference type="OrthoDB" id="306829at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8188647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="367" mass="44312" checksum="8797547F0EB83283" modified="2022-10-12" version="1">MELKNRIMDAIVMWKYEFYNEEVQNSQVEGQYQSNEGQYDNFDDLFHIFDFGLSHDLQYLAIFEDKIRKKNNTSRRFKGNQKSDLRIKLYEVKSNKVIFESSLLCRGFLNINFHFSQDSNFLICQGYSDGYLIIDIQQKKEFLFNNLEETCFIFQMDTINSYYINKNDKIFHINVEQINLQYEKEIYLKFNFKFILYFKSFLMKFALISTELFQYIIYLENCKVIKQRKKQTNVRTQLIIFQNCLIIEQEDIYETEYSVRLLNSGKILRRIKNLVGNSGNISYDDYGVYKNSFLSIENNNHQQIKIFDFLRGNSKQISFIINSQYKDYYQISKLYNQSIFTMIMSGLYTQITSYILRQYQYLKQLEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WJQ2</accession>
    <name>A0A8S1WJQ2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0930074</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000093">
        <property type="protein sequence ID" value="CAD8189182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WJQ2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8189182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="323" mass="37810" checksum="76447E6E6E5E95CC" modified="2022-10-12" version="1">MNYTVYSPNKKLVYQNKNGQQQLLSPDQKYDKKVQGLLKMIDSQFENMLHYQSSIENKPNLRTEPNEYQADNYPTKTVRHFKSQDKNSKTVLANLLNLVDSKVQKSTKLNHSISNTINDNWLTKNKQLIKTDQSVDLKNDQLQDRIKTQISNYSIQDASVQTQSSKQKKFGFFTYEISYISPKCKNCGFEKQMIDDRSIQNQRAESNYGNAFKEYSKNDSIEKCQFSKSNNKNQHFLFDQKIENQLLGINSITLDQKNYSSNNLKDLSLLQSIKKKNKNLKNFSQLAQDVQIFSPAKQEHFINTNIKQQKKQLHNHISQFHKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WL00</accession>
    <name>A0A8S1WL00_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EML-like second beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0960015</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000096">
        <property type="protein sequence ID" value="CAD8190243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WL00"/>
    <dbReference type="OrthoDB" id="674604at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25173">
        <property type="entry name" value="Beta-prop_WDR3_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="215"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="257"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="298"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="340"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="382"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="424"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="466"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="508"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="516"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="550"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="592"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="634"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="682"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="717"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="759"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="801"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="843"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="885"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="927"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="969"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="993"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1011"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1053"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1095"/>
            <end position="1136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8190243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1216" mass="134849" checksum="A3A8C7EC05E1DE8F" modified="2022-10-12" version="1">MDDPEDLSEIFAQVQGVDKRIYSLIIQMQEKQNTRDWMGFLSDYENLRHLEEQISQKEDSTQEEKEQRMDVMRNNIKLILNVIVKIKDHDFNKQDYSDQRYFAQRQDLIKRIREQNEIIGFVKFLVNLTAYDENLIQCGSNSLNLLVNMKVDIRNQNFENIRIKNTSLFGANFFRCNLSGSQLDNVDINGMNLNGALLLNCRWTNVKKQGEINKLDGHTDSVKSVCFSPDGNILASGSEDDSICLWDVKKGQQKIKFDGHSSLVHSVCFSPDGTLLASGSYEIIGLWDVKKGQQTKILKGHGDRVQSVCFSPDGTKLASGSKDTSIQLWDVKTGQQKKILNGHRKTVQSVCFSPDGIILASGSEDNSIRLWDVMTGQQKAKLDAHKNSVKSVCFSPDGITLASGGWDKFIRLWDVKTRKKKVQLNVDSQYVQSVCFSPDGTTLASGSYDKYIRLWDVKTGFQKTILDGHTQSVLSVCFSPDGSALASGSCDRSIRLWDVQTGFQKTELDGHNAPVWLACFSPDGTILASGSNDKSIRLWDVKTGQQKAKLDGHTHSVLSVCFSPDGCSLASGSLDNSIRFWDVKTGQQKFQLYGHSSQVTSVFFSPDGTILASGSWDNSIRLWDVMTGQQKFQLYGHSSYVTSVFFSPDGTILASGSYDKSIRLWNVKTGQEKAKFYGHSNVYSVCFSPDSTTLACGSEDNSIRLWDVKTGQLMSQLDGHRYSVYSVCFSPDGTTLASGSMDSSIRLWDVTTGYQKSQFDGYTYQVLPVCFSSDYTTLAFDSGDKSTCLWDVRIGQQKTQLDGHSILVNSVCFSPDGTILASSRPDKSIRLWNVQTGLQVTKLDGHSATVWSLCFSPNGNILASGSTDKSIRLWDVKTGQQQAKLDGHSGYVYSVCFSPDGTTLASGSEDNSIRFWNVKTGQQKAKLDGHSSYVYSVCFSPDGTTLASGSWDKSIRLWNVKTGLQKIKLDGHSSQVNSVCFSPDGTILASGRADKSIRLWDVKTGQQKNKLDGHRNSVISVYFSPDGTTLASGSSDNSIRIWDVKTGQQKIKLDVHKNSVNSVCFSPDGTTLASGSSDNSIRLWNVKTGLQKTKFDGHSSFVLSICFSPNGTTLAQSNSDNSIRFWEGKTGQEIQSQDKNYKDILNYSKTLLIENSPLPGTTYLNILLISQQSIFQAQDALVLKSEFVNKLGINLQTLFKQKGSCIFESQLDSKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WL63</accession>
    <name>A0A8S1WL63_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0940199</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WL63"/>
    <dbReference type="OrthoDB" id="290102at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8189782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="214" mass="25467" checksum="9E4AE3593CC28A7C" modified="2022-10-12" version="1">MRNSQTTPMRRSITLQSRIMQKSERTLLPQEDILKEKVKSDFALGHFRGLMSRKTPQKELPKTEIQLKRFEQFGKANQQSKNKRLTFLKYGFRSHEKKNNYYLQPFIRKVRDAEGDVANDGSMGVVDLWSDRCINKLMEGMHMTRDNLIVRKMGRELNDSDQQMKQKLEERRMKDKILIESMRMASQDTDTFAFKFQLPSKNKKIYTKKQYQSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WM23</accession>
    <name>A0A8S1WM23_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0940110</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WM23"/>
    <dbReference type="OrthoDB" id="289008at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8189605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="110" mass="12553" checksum="17DE658A2003A932" modified="2022-10-12" version="1">MVSCAQYCMFISAPGCIFMTYLGLMIAIGSETVYVVPHSRIEGAFALFITAVLYAIFFAISYNSEYSQIQRQQNVDEWINSQEIREAELEMQDIPKQPKSNLQESIEQPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WNM0</accession>
    <name>A0A8S1WNM0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Leucine-rich repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0990124</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000099">
        <property type="protein sequence ID" value="CAD8191608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WNM0"/>
    <dbReference type="OrthoDB" id="289282at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050576">
        <property type="entry name" value="Cilia_flagella_integrity"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973:SF9">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT SDS22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8191608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="276" mass="31461" checksum="A2441EE7D34FE615" modified="2022-10-12" version="1">MNNDKKNDKKPVKPVEKSSTTNNQQIQSKLLGTNVNEINIEKLRDLKQTQNFNLQGKLTSQIVKTVSNKFGLDIVFVLDLSNQKIGNIECLTECIHLVQLNLQNNSISDISPLKVMKDLAFLNISNNQITQLDLGELSSLQNLQAKGNFIKSVKSIEYLDRIRVLRTLYLQCPSGDQKNPICDLNNYRNVIFNTIKALQRLDGYTREQEYNTGQELQKEQVQIKIEIPNTGPWYVQQFPKVDNPSSVQLDDKGVKQSIDETKKLISNIEMKLALLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WWD0</accession>
    <name>A0A8S1WWD0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1060048</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WWD0"/>
    <dbReference type="OrthoDB" id="308520at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="305"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="344"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="628"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="305"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8194148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="733" mass="87158" checksum="727378496346FB21" modified="2022-10-12" version="1">MDLQEELNQKDQTIKQLEEERNILIQNTNELQTNQIALEKEITRQKSNIANYEAELIELHKERKSCDEQIQKSQIQIESYEQQITLLNVDLKKVTIQCDEALQKLQEIHTRHEIEIKTLFDQLMNEKEKINEINDDYQQILNGKLSEIDLLNEKLNNLEEKIQTNLKNYKIEIDQLNNQIEQTKQEKDNEWEQAIKLLQNEIDDLNKALYEKGQNNTFEQKIEELNQYIIEQEQQYMKQFETQEGKMKKLQDEIIILNEQILELKNKNENQLLDLTALQQELIHKNESCQTLTQQLEDIKQQQQKQIQQTNQNHNNNLQTQHSNTAEKMVTIPMKELLSWDKRSKDQQNQIKALSNEIQLLKNSQEEQQKLQNSKQMKSSLSLEGMVQKCTALQQIIDETSQQNSKITQELNTYKLQNQQQKEDLHNIQHELKELRVIAQDKFKLEGELASAYIQISENKDLMKKLSELSSNYENKEEQLETTKKTLRQSEQLRNNLQIKFDEITIQYQKTQQELINVKQERDQKIIKLKDLEKLLQSQFQEYSHLESKYKDSQINYEDLQSQVKILQKKYSHETEQLKDDCHQKQKQISVLEESNKCLTNDLLGLRELQFRQKAPEAPLTKKEKIVVESLSLRVQELEMENRKTRDTLCAKIVQLQNELEQKTNYINQIAHQISKQIPSLQEQDLLDKEDIKMIQQLKKKVNIQEILEKTLIENLQLRDQIKLLGTEINKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WXT2</accession>
    <name>A0A8S1WXT2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1040080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WXT2"/>
    <dbReference type="OrthoDB" id="294634at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8193427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="158" mass="18800" checksum="11F0CFF40C395467" modified="2022-10-12" version="1">MNNPFQSPIGNPNDPNNQLLLMSLAQVDSQNMFFFLMQNTDFVEKQDENQFKKTAIGFVRNTLGIFFLGTVLNLQMKRIPKFLNWPIYVKIPLRIPVFFSPFFLFQQSLTNQLDNLTQLHHKYYTRILRVKKTGDLRYFDPQGILQKQFEQKMKELMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WY62</accession>
    <name>A0A8S1WY62_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1030132</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000103">
        <property type="protein sequence ID" value="CAD8193149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WY62"/>
    <dbReference type="OrthoDB" id="293533at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45911">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45911:SF4">
        <property type="entry name" value="MULTIPLE C2 AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="335"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="383"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="425"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="6"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="43"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="4">
        <location>
            <begin position="96"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8193149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="492" mass="57849" checksum="ABA9099D4AFE69F4" modified="2022-10-12" version="1">MATFQQKYESLKQEFNELDINDDGKLDKNEMFQVLDSKIGKQFDREIAEELWSNLDINHDGKVTVNEFIQILLKAEDSLKSKINNCRTTLELNYQQKRETETNLDYCQSNEKLNNFGVMEGSQLCVIVIQAANLKADGKQLDPFVTLQFDKFEKQTIINKGPNPIWKELQKFYFPVQTGKEDLKLTVCDFDKYTPNIVIARVDLNLSNNSFNLKNQQLHDIWLDLYDINGNRSGSQLNIQIQWVYSKVKYLRDVLNSYQQQVYQQEQELLQYERDLFILYEPFVKWRQLNTKGYQKPISQEPTSIIPISYQQVDPTKIVKPFCDPLLDKNSKFKLITILIGLIGCFTLLILFYKAQFFDLLLIIDFYILWEFNALNENRIKTLGILFGMSCFLDVIWIFCIGMKWLGNDSHEIYIPYEISIQKMISILVIISLILRLYLILNLFQLSQEVQKITNPQSIKIQHKNIVKIDMQNTHNIESKTYLHNGRPIVIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X0V8</accession>
    <name>A0A8S1X0V8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Vesicle transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1070046</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="8">May be involved in fusion of retrograde transport vesicles derived from an endocytic compartment with the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the SFT2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000107">
        <property type="protein sequence ID" value="CAD8194557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X0V8"/>
    <dbReference type="OrthoDB" id="73614at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011691">
        <property type="entry name" value="Vesicle_transpt_SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137:SF6">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8194557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="155" mass="17676" checksum="A98F6BF4F89DEB2E" modified="2022-10-12" version="1">MKGDYNFFSDKKDDPLDAVCPKLTYQQRLWGFLICSGIGWFLEFCAFISFFQGNGKEFAIIFSIGNLVAILSTLFLSGPKDQCKKMADKSRLISTIIFFSTLTVTLVLAFATDLTFLTLLMTLVQFCAYVWYVLSFIPFGQKILKKFFSSCCEFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X2M5</accession>
    <name>A0A8S1X2M5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1120100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000112">
        <property type="protein sequence ID" value="CAD8196384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X2M5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8196384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="118" mass="14279" checksum="C0287290FCDE5406" modified="2022-10-12" version="1">MQSRTNPIILILRYLNVQCKYFPFIKLQIYYSYFSHKECRLIKLKIRKNITIIPYFNLSICKQYFFFIPVITQFNQINPFKTINKQTIVKSKVILYQLQQFLNNIIVSPKLRCLACEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X5U0</accession>
    <name>A0A8S1X5U0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1130048</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000113">
        <property type="protein sequence ID" value="CAD8196597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X5U0"/>
    <dbReference type="OrthoDB" id="294565at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024943">
        <property type="entry name" value="Enhancer_polycomb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14898">
        <property type="entry name" value="ENHANCER OF POLYCOMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="388"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8196597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="602" mass="71339" checksum="E2BB0CB62F07779E" modified="2022-10-12" version="1">MFLVFLGIVSSYIFKFLQLLIDWAPKKMKSRPRQIDPTKPVLISNSLEAFTNAEGQEADIKNTEQIITPDQLLKEKQIAYNQSITYKEQVEHFQPTLLWFVTTCFSTPAIFIFILYSLLFRPKQFEEPTLKSQLPAKCREDYISYKAPVYPDGTAYELTEEDIHFLSTINEKLSNPVHIEDFEQYLSLLDQKSGKDVEIDFLEFLKLKLPLPKTINIQMQSTQEQLYAYFKKQRQHFSRPLTRYLMHPYYEDNNPHLAFRPRTQPMDRTMKLRRGNPQNSRGVIEMENLGQKIVMLKKDLLMVSNLIDQSLQREKAMEIDRRFTFCKFIKEFFDQSKREKDNNSEIKLWPKDCFVRDCSEKCNINILEKSEEMFDYITKEILQWKEQNEIQRREKLKKEDQIVRYEKQIEDLIIEKQKYEEKPIVQPPPVPQPPIIPPIIPVIPQEPPDEKMESVARFLATLYLEARKYQLTFDQVLSDSFPIYKIQNQQQPVNSKSVFPFSLMQIEGNDLVYKVRNDSYLEKKDKAYWENYTDPSLSNTLYYQNEYFKEKVNDIMEMADMDQMVYAQGLDDDKQDEDDKLFKKKVKGSTTRLTGSNGGQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X659</accession>
    <name>A0A8S1X659_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Intraflagellar transport protein 46 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1060012</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IFT46 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X659"/>
    <dbReference type="OrthoDB" id="2119217at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030992">
        <property type="term" value="C:intraciliary transport particle B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022088">
        <property type="entry name" value="Intraflagellar_transp_cmplxB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13376">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 46 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13376:SF0">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 46 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12317">
        <property type="entry name" value="IFT46_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="25"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="29"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="46"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8194076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="298" mass="33802" checksum="3A8EEF059D549B1E" modified="2022-10-12" version="1">MQKNDYGFGGDEKVKKIDNEHYDEAIELKDEEDEEIVSGEEDEDQYQAGARGAQQAAQSAGFQDGKVPLPAGAYNPNDYSNLQVGAEVKQLFEYIARYKAPQMVLDAKLKPFIPDYVPAIGEVDAFIKMPRPDGQPETLGLVTLDEPALNQSKKAKLDLLMREYVKVANRGKNTTIHAIENADKNPKEITAWINDVAEIHKKKQPPSVSYSKIMPDIEELMQVWPQEIEDLFQSTQLPGDNIDMGLQEYCQFSCNLLDIPVHSINNNRNIIESLHVLFTLFSEFKTNQHFRQNNDEIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X8Q1</accession>
    <name>A0A8S1X8Q1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1150067</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000115">
        <property type="protein sequence ID" value="CAD8197360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X8Q1"/>
    <dbReference type="OrthoDB" id="306183at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8197360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="166" mass="19735" checksum="7C7F1060438B31B8" modified="2022-10-12" version="1">MNSKNYPKANRRISLKEQAHSPRQPKLNKNKEIESQKEISSEFLIRQKLYEDLNKFPNYESENLLKISERKESHYNKFRTIIEDAKQRFINSDSQQSKNYLEKQQSSMKLIVEQLRNQKRLQNQVHLNQTQNIESINLNLVLSLAIPILCLIVVFLELLKQELIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X9C2</accession>
    <name>A0A8S1X9C2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1160001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000116">
        <property type="protein sequence ID" value="CAD8197577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X9C2"/>
    <dbReference type="OrthoDB" id="312869at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8197577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="245" mass="28138" checksum="1209A4E44E52913B" modified="2022-10-12" version="1">MFQKIIRGQLQGFQAQKWSIMTLQDLQGCLKLLDGKQVRWSQELDNVSFLIISLYVYGMSKQDNKNKSQMVIDIMFDHSASLLMEIHQHLVVMITLFVYGISKQDNKKPNQMVILVLSRQYVSLLMETHQLLVVMISLSVYGMSKQDNNNPNQMVMLTLSTQSVSLLMEILQLLVVGINLSVYEMQKQDNKKPNQMVMMNLLHQSVGFGKGVLDQIQQGSTRLDLKQIIIQSYLRLGLRQMVPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XA86</accession>
    <name>A0A8S1XA86_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1160105</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000116">
        <property type="protein sequence ID" value="CAD8197772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XA86"/>
    <dbReference type="OrthoDB" id="304475at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8197772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="336" mass="40000" checksum="C6EF67268B82DC66" modified="2022-10-12" version="1">MRLNNSTKRTIEKQKKLLIKKIMSWVRQSFNNRQIGFHPFVLCVSIKPSINQRCATIQSYPNQELWKSLHSLQDQQLLQQIIQEASEKTFKLKEKYDNSIIQDFLNRIKNNFDFCKFYKDTQDINFDSDEHVSNSTELNENFQIKQFQKKESESEQLLKQIELCNSKKGSIITIEKQHYHKLSQLFEKICLVPEQLYSIEMKKFKNQLSLLLESTQNPKEKQICVQNEFQTEKVPNSIQNLDIYVNQLKNKFQQEIIDEVLQIAPGLVLFLPSFENTQVNNDELQQVEQDFFKEILYQKIMAPQNLTPQNIIFAWRYGFEPKIKEILDKFNKIKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XGL2</accession>
    <name>A0A8S1XGL2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1230054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000123">
        <property type="protein sequence ID" value="CAD8199988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XGL2"/>
    <dbReference type="OrthoDB" id="364513at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019776">
        <property type="entry name" value="Flagellar_basal_body_rod_CS"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00588">
        <property type="entry name" value="FLAGELLA_BB_ROD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="916"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="942"/>
            <end position="964"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8199988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="964" mass="112488" checksum="2F102031B5E0A352" modified="2022-10-12" version="1">MIGNIIQAIGELLNCKVDFLCTAQLTSDKKYFAVVASDRLLFIASKLEKVEMSLEYGKIKGIVISTRVPTMFQIHTDIIIDVYALERKQLIQALRYSWMTDFMHKNFQFKQLPMFKDDIKSNIQDKQPVLPNLMFTKQGKQMFRNKNYTFSLPDEYEATYQDGVYVHKESQQCKIVVQITDPMPTQLLSQLGEKANLQYYSEMFLEIVLKGINSKDDSVKQGTNQKSQDSDEEGKKQEEDEQNKQKEETLDYWIISSHNYKKRSNLMNDISEWFGWELHARTQVQDIFIIILRRIHIPPLFETYQEILFVLYGQEQNAKEQVLVNDRDLLKLTLEQDLEEHAFKNVLRNAIDSLQCKMFIFEKQYQIFIQLSADRLLYNRQTLNFIYNKFKIFPQFMSYALKYLKLLFQRLVLYDKERFLILDKELTQKYGFILNNLKDYMTDGIQIEENLQALFDAFEQASFVRDKSGNEEERKFVWQQKLSNYLGICFDGLLMGSAFSFKDLVPVLQAKVSMELKTIILELFDYCLYAVPKKECRPIKKGNFIKKLEELASNKYQYNVNIAKSLIEANFFSQEVLLQERSGLYSSLICYFLQSDNVDLIVAICRHIINFQAEIKHTTGLTEAMITAFKPISVELIRLYRSHNRTIATLACASLFNLCTNSREFKYIILNDDGAALLVSKLVTKDNFLLHFALKLIYCVMSITQNIEKLLAANLTDYLYKILEGPQIKGCQYDARCLITTCQILSVCLVSEVELRNIDHLLILLYNMVTTQADQYFLDQEESVNLKSEALYVMSKMCHKSAEVKKKINQECVPYLLKLMEKLTYHQVQEKIIMLLCILIKENKEWALEWLQMQIKLQDILTTYHNNDIQSSNYLLSQIKFIDDEAKKVKNEEMKRSKLAPTASVIAEQMMEQLQQQESTIQDNKKSMVSEARSSMIEDSRITASNINNKSTINPSQFKKSSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XM40</accession>
    <name>A0A8S1XM40_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RanBP2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1290088</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000129">
        <property type="protein sequence ID" value="CAD8201907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XM40"/>
    <dbReference type="OrthoDB" id="282959at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8201907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="99" mass="11681" checksum="CD7A179230434E5A" modified="2022-10-12" version="1">MNSSPYIVKRKQRVGDWICGVCKNLNFSFRNQCKSNEQYPGNRCNKFPHRKSSKQQFRGFRTLVLIHYEEPIESVDELLENQIDPIVKSSLLVLDMDQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XN73</accession>
    <name>A0A8S1XN73_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transmembrane protein 234</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1310060</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000131">
        <property type="protein sequence ID" value="CAD8202483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XN73"/>
    <dbReference type="OrthoDB" id="43458at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018908">
        <property type="entry name" value="TMEM234"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28668">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28668:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10639">
        <property type="entry name" value="TMEM234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8202483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="13377" checksum="822D7415712B997E" modified="2022-10-12" version="1">MIYIIGLLVVGIIWGITNPFMESGVGNKQQDLFGFKSFIQTILNYKFIIPFGINQSASIFYYYLLGHTPLSLGPVIVNCVNSATTVITESRLKKQKLKQKTWIGLILMFIGTYMVCSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XQX3</accession>
    <name>A0A8S1XQX3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1330179</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000133">
        <property type="protein sequence ID" value="CAD8203397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XQX3"/>
    <dbReference type="OrthoDB" id="307939at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="184"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="346"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8203397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="389" mass="45718" checksum="41BAFA2535801E1C" modified="2022-10-12" version="1">MYQETQNKKITFKFLNQLKLMIKPSQKVIQISHALVTLLSGTIASIQGNQIKSDWQSIKYCLRNTNEILEIINDSQTFQKRVRFENLEKSMQILQQIQQQNEDEQNDGTFELIQDTLNQLLAIAKSLFKNLLLCKKSNSRNRSLSQQGFQKMNKLQQSQKSFSNKTNISQSHLHQSNCKINRPKSQQSNLSCSRSSSKSKDYQKRVIEAQTKKQSRLDRILKLQTNKPSATSQIKQMIEGQQSLNRSYRSNSKESLMRQEIEKNKQIIKKMKQQENAIKWEIERESKRIRENDIVNQIQDEINRYKVSWQKDQEIKQEKRKSNTKQNSFNKQNNTNNGTQRNNSINRDENKGSNLEKDLLLSEKVKNEKEQSLSKILKTINNSKQKYLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XR24</accession>
    <name>A0A8S1XR24_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1350106</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000135">
        <property type="protein sequence ID" value="CAD8203936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XR24"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8203936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="318" mass="36487" checksum="9BC1293017E5257A" modified="2022-10-12" version="1">MKQNFCPDLHVTVPDMDLHICVSYAEICKDVPEVDILVAQNGTQYVCHPRFQPFAPGTPTIDFFGSINCGYEEGVQMVIEQLLDYHLKFYCKFIEGENYKNCLATEIVNGYQRCAYCKAPFVGESCLKLTQGFYDWRYFNPPKKCESITCIECDNPSSCTKCAPGYGSTTPANTCDKWLDSTPPCKEGTYLETVDKCFFCPEGCLSCRKRSASTQPSVTCDVCDSRYFKVKCRQDDPDCYFCFIGTGCDFDRQQILTDYTTLNALGQYQYVISCTQCDLGFFYSKEKKKCECNIQQYFEILRIFRVIVQFMTQFQKNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XSL0</accession>
    <name>A0A8S1XSL0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1360004</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000136">
        <property type="protein sequence ID" value="CAD8204075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="NACHT" evidence="3">
        <location>
            <begin position="604"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1348"/>
            <end position="1389"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1390"/>
            <end position="1422"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1463"/>
            <end position="1504"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1505"/>
            <end position="1539"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1547"/>
            <end position="1588"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1589"/>
            <end position="1630"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1631"/>
            <end position="1672"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1673"/>
            <end position="1707"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1715"/>
            <end position="1756"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1757"/>
            <end position="1798"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1799"/>
            <end position="1833"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="439"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8204075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1922" mass="224554" checksum="73A0709C2DED5553" modified="2022-10-12" version="1">MKMIQNEYQNKFSNSKGLIEYLIVILNLEEQQNDSEITDLKLLEKEFGEDFEEASLHQLIENMQKIIILINETMKKTNEVSFNQQYQKFELEKYEHFLKQFKILLETNNNKKLIVVNLEKIINQLQIIVQILNKALKQNFEIFQILIIEELGKLREIINQQNYILLKYQENKIQQNNQKNYINSINIILKILQIQQILIKEQKNYLVLFLQEIVDFSSYINLIQSYNVEKQKDITKFFNLSYQKQINNFIELQYTIRNLKQDIQENESSLNYLERILIDLQQDITQIKKDGGKPNIYDLLNKQKVKIQNQLEEKSYKLQELFIEQEFYVLQIYNINQLDGLTQEIDGESNQQLLLSDFWIQKLKHNKKNEEWKIKEGLVFAIIQISSNCFTDTIISFCQKALIQIWAKEKDQRIRNLLKNKRIISLQMQLLQKDWKTQHDRIADEMQNMLNKIDDLQEQISHEINHNKRDQQLQKLDQTTQQLDEYIENISDMGQQLKLITDFLNYIRKGLTRFERKINIMKEQLNSMGNDIKFLRGKSVDQLFEIRKWKVLKEAAQKNIKSIYIPLKTQEKGQLNPSSLINLEQFDDKVGEVNEFLLDEKETVLLIHGVAGSGKSTTAKKLEEFIWKLHDNNIKISNQILIPVYISLPFLKNPVFQAVEETLHQDEYGFDNLQLKECKEMLEKKEFRFLFIMDSYDEMKLENIKKIYILLTNLIKIGQILWSEILTTSNYKEWFVPEDKKQIKEVQLLKFDEMQKKEYLTKFTLLSIKVLVFEIYEWQTQMKYQSSIDFQNFEKSWEKLHAEVLKQFISNVNSESLLNQKQIENIFLFLKNDNHIALKSIEAIRSLGFKLQKLWSVRKYENMIKMINLDKLIQTPYMMEIIVSVLPLMMVKKTEIINLKQNFIKNFSKMYRELLKSEYLIKMYANHNQNQEASRLEMQFIEKYDYIQTAIEIWNKMEENQIPNQFQISQEINELNIKLIKIFQHNYKNILGIKDEIIQKVQINFERLIELICDALKEQNLTSYDFYDEFIKQYHLKQIEKQRNLGKSINTDRFLYDLYKYSIKLAKQMSIKEVTQVQYKQQGLLYKVENEEEKWLNEFFNDDGQDGSYKIDIRSCSLIQQKGANFQFVHKSIQQFLISADLYELLVLSKDLDVKIFSKLIKIFIYEKVIDCLGFLQNDVNQNHYKFCIQLDNLSLYDKQQKLNIIENNIKNITNIVRMLKQHDLNLINYSAEIYVETRKQLIQQIYQEKNIIEFLKFIVHLTAFDQSFIQSGSNFLNLLVEMKVDLTNLCFRNIKIKNTSLIGASFVKCDLSESQFENVKINGINLNGAQLFNCKWKNLQMNELHKLEGHSSDVNSVCFSPDGTMLASGSAEKSIHLWDVKTGKKISKLEGHSHWVNSVCFSPNGTLLASGSWDNTIRLWDQNLNLKNKRSIYNQFVSVQMAIHQHLEVQIILLTGQEKSKLVGHNDSVFSISFSPDGTKLASGSADKSIRLWDLKTGKEQQKLEGHNESINSECFSPDGTTLAYSCRDCTIHLWDIKAQVEKYKLEGHNSYVLSVCFSSDGIKLASGSWDNTIRLWDVNTGQENSKLEGQSDGIYSVCFSPDGTILAAGSADKSIRLWDVKTKQEKLQLEGHSENVNSVSFSPDGTILASGSSDYIICLWDLRKGQKQSSLEGHSDSVISVSFSSDGIILASGSRDNTVQLWDIRRLQEKFKLEGHNNFVYSVCFSPDGNTLASGSWNNTIRLWDVKKGQRKLQLKGHIKQVQSLCFSPDGTILASGGWDNAIRLWNVKTGEEKFKLDGHVNQVLSVCFSPDGFTLASRSADKSIRIWDIKDALSNQISIHSNQDILIQNSDYQDPLGIISNILTIVLISQQGLLEAQGTQIQKGSFTNHLGMDMRIHFKKKGAHLLEHLLESEQNAVQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XT17</accession>
    <name>A0A8S1XT17_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1350092</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000135">
        <property type="protein sequence ID" value="CAD8203908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XT17"/>
    <dbReference type="OrthoDB" id="301689at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8203908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="522" mass="62038" checksum="2213C6EC96803E5A" modified="2022-10-12" version="1">MFSEIIPSLMDYSQHLDRTIKEVQQQILQMETITNKTNSHKRPLEMKYLQHKSSVPSTLPDINNDIDKILLTAEQFLSDPNKTQIEPKKQEKQDFEQPKRKIESYKQPTNKKQILSRKVSQKKLSPSKVSMHKRSGSLYKQEVTPKVTQVQPKFPIQQRESTKSFKDQSRLIKQSPTKSIHEKNSILIEDQSKVKPPIPAVQPIRKQPSQPYLQLQPIPQQQQQQQNLNSSYTQAPINNKMENNQQQQQNALKKFDFNGYKELSRLKRMKDAIFVEYQQSYTYFREKEQKSRARFLTKFHQHMNERKKLLQQSAYGDRQTFFFLSDQIHPQYFSFIEEQLTIGQPKTLLILKYCEQIQKTIDSIEFDDELLKNLKRANKQNISKISLTELVEVFKLWSQVQIIRQNYQQLTQFIQPIPELTYDLIQWICLRPKIIKKDKTQIQLSQKVIRYNQFASSQEEALETNKLIIKEEIFRFLWDIIQKLVFSQQQLETSMALFKSILKFLTKNNCSVYVYKNSDLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XUS5</accession>
    <name>A0A8S1XUS5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1400076</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000140">
        <property type="protein sequence ID" value="CAD8205286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XUS5"/>
    <dbReference type="OrthoDB" id="286463at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8205286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="184" mass="21428" checksum="F1B58258EBC3948B" modified="2022-10-12" version="1">MSRRDQIEKDKQALLSKPWVKNHPIFTYDHIAEFYEMFILYAEPRTKKADVRDILVTAKTLGLTDKFPIIAHALDDLAASYDDAIDFETFISDLTAKLGNPFDQKGRVQLFKLIDVDGKGTLDKGDLQKISEELRFNLNEEDIQEIIHNVAGYEAEDVSEEKFEKYLGKRVQRRQVEQEIYRNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XZL8</accession>
    <name>A0A8S1XZL8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1440018</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000144">
        <property type="protein sequence ID" value="CAD8206407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XZL8"/>
    <dbReference type="OrthoDB" id="287390at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093:SF18">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="422"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8206407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="617" mass="72404" checksum="12ABCB2248FBD779" modified="2022-10-12" version="1">MQQLFWKKIEIDNKKHGRPPKLEECVNLVYLIDQNRYLYFGGKNPFNIKDLQQEEKQPSPPKSDGISFKIGAPKQARVSSPFSNQECQDYVYILDPATYQWSIKKCTGKPPTRRTSAQVWYEAPLLYVYGGITYENKTVSDLYILNTEKFIWKRFFYLEGPPGRLHFGFSTQGIKKYLMGGASMPENLLMDDVWQLSFENVAWETQSLELPGITWEKLPFDDMPAIKAHTMIQVSDKELLVYGGFNKQKQCQDRCLLLDIETYDISQLELKGQSPGQRAFHELLVVKENLLCLFGGYNVNENQLKNSEPLNDLYLLNLNEGYWSKPIAGGYIPTPRFGYVMSCNQNEVHGEIMLLGGRMNDGTVDQCIYVLHELDMEARDAWDEKDEKEKNKYDTYRELKKENKNITVPTLTLVFDEAEKIILEQKRTIGEKEKDLKQLQQNSLSLEQRAEKLQEQIDVGKLKMDQDLENTKIEITELNQKADQSQSTIDKILQLLAFERKKRKLMYRKALALEDLYRKTSLYIFEMDKVFVKGQSENLLETSINEDILNLIDQRKQEYKNILISFHKSFEERYKQEMQVRQQIRSNKDILKQFHNINEVYKKTLLKEDREYLDKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XZM8</accession>
    <name>A0A8S1XZM8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1440028</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000144">
        <property type="protein sequence ID" value="CAD8206427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XZM8"/>
    <dbReference type="OrthoDB" id="284828at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8206427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="543" mass="63750" checksum="6E35C04967713A54" modified="2022-10-12" version="1">MFSSESMQEHILYDKIYQYLWVKSETANPPAILIPDTVILIRSMPIYWYYTDKETGEIKKKMRKNVTKQNIKQTWNQQVGRGGVVAYLLHFIDNLDLNYPPEKFQISGQIQVIYFDVEGFNKFMDGHFELPFGILQQFIEPADDRNSQIQAFWSKSVTLFTKRVCKKSFTNKSMNIYERHCTFEGPEYFSEATQVKDFQSQRISEQIDKMISHLDAISYGKLNISQGTFYFKVDKKARCWFLFCGNLKFEDDKQLKNLPKDLYTQSQIKIPKSIDNLMSVYNSRPLQLNREHKCIRCGQLEKGSNFVEVPYHFLIDLSENPLPVEQWPLEVKKQAKQVKLSGANQEKIAYVNTISQIPMTLQKIHEKLNIQNYQELKQSQGFKYKTIAVCLDCYFIMVENQERIAMQEVTSKGKTSMPRVKSKQIQRKEQSLNQFLNSTQLFKQVKSVKNIQIKQVASQTPTPKQNTIQKYVSLRLTNCKSITPIKYSIEVPSTADYLSRKTKSTYQDMSSTYKFKNSRDNYNQSLQERQQNRLIEIKDEQEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XZQ3</accession>
    <name>A0A8S1XZQ3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T1450125</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000145">
        <property type="protein sequence ID" value="CAD8206926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XZQ3"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019530">
        <property type="entry name" value="Intra-flagellar_transport_57"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10498">
        <property type="entry name" value="IFT57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="12"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="634"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8206926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="678" mass="79303" checksum="255518EA3F2F5108" modified="2022-10-12" version="1">MSQFIDQEINGYKVQKAIGEGKFSTVYKAMNKEGNVVALKKIKIFDMMDPKQREKCLKEVKLMQPLDHPNIIKYLDSFIYNNELIIATEWAEKGDLKKLIKNAQSDDTPFEEVQLWNYIMQIASALDHMHEKRIMHRDLKPANIFIGGDNTLKVGDLGLGRIFSSETIEAYSKVGTPLYMSPELLHGEGYDMKSDIWSLGCIAYEMAEFKSPFKQSEKMSLMDLFNNITKGEFKPVSNRYSQQLRDVIEGMIVVDPQKRLDASTVLQKAKEIHDTFLDTKKTPQIINVLMMEDIYEKLSLVQYHEYFCIPLKKKPVSKYYFSLDENVNQNQRFYYFVELCYWLMSLPKQKKSKMAQPIKLNYNNVEETARKLLIDIKAWGIKLPEQLGSPHISQGCGDMVCFILNDLLNRELIRVNFKFESPNFGKDMESSVIQVNKIDDEDLAEIQEEGNENEEVEQDELTQSILFYHKKYNNEQTDYNQVPQDRQVIETKVAINEWTKEFNRVEKEFSKFEANLKVNKHYLKDYERTIITISKCSKQLSLLSEHLKSNEVQQIQQQWIDYLELLPKLETKVTEQIPEDVQITLKQNRNTISDLQLQISLLSKGNIEKQEIDQQLNQQLLDIKERTPILVDNRSKQKQLINQLRNDIRDMNIKIGIMQTQISKSNFNTNEVLSDEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XZX6</accession>
    <name>A0A8S1XZX6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1440078</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000144">
        <property type="protein sequence ID" value="CAD8206527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XZX6"/>
    <dbReference type="OrthoDB" id="437960at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="19"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8206527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="387" mass="45058" checksum="7938EBA8D3BFCAE5" modified="2022-10-12" version="1">MGSNCATCQNCQKKEEVLISQEEQQSSRQMNEYSQQPNEEDDDDDHLEDKNLDASSQIVQKYLNLDLNQKKEVVPIQSVPAQLVEIPQAQIVMTNESNQDSKKRKNEMMLRNQEDMHETHISKSENSVRERRPRHIFKSGAYYDGEWIGQSRDGYGIQIWSDGAKYEGEWKNNRANGRGKFWHIDGDFFDGEWKDDKACGKGVYTHLNGAKYEGDWIDDLQHGFGVEIWADNAKFEGQYQNGKKEGFGKYFWADGSSYIGNWSENKLCGFGVYTWPDGRKYLGQWANNQMNGRGIYLWKDGRKYEGCYLNDKKHGYGIYIWPDGRKYEGYWNNGKQSGKGRYVLQNGRSQLGLWEDGKRIKWLDQSEQNIDISPKDWNQYVQPSLVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y1N2</accession>
    <name>A0A8S1Y1N2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1480141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000148">
        <property type="protein sequence ID" value="CAD8207829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y1N2"/>
    <dbReference type="OrthoDB" id="306613at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="23"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8207829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="194" mass="23188" checksum="AF12E653296DA5E6" modified="2022-10-12" version="1">MSEEDLIMTKMKLQTYKITKDSNDKLKTQISENDEQIQKMRDLIEEIKQKNIEIKQEFVNQDLEIQNLGKEFQLIKNENDAQIYKLKDQLIKYEQRYSEQEKINENSIIQQQFKQPNRYFCDIQRSKDGRKEAKIVFDQSFKSKKLLTDIQQNSLNFIVYLENNEISHVIRQQIQLDSENNSIFQGNEVTVKIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y4G6</accession>
    <name>A0A8S1Y4G6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1510047</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000151">
        <property type="protein sequence ID" value="CAD8208530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y4G6"/>
    <dbReference type="OrthoDB" id="308108at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8208530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="417" mass="48259" checksum="4A6BC4863D339C75" modified="2022-10-12" version="1">MYKSITVQTLPPEQIRQFQVLLTFQYVNNLLDKCAEFLQYKVQLFQDKECKYEIDSQLSIEQAKLNNIIFAKRIQSESASSYYPKVICWFKQNGVELSLGVMLSSDFTFEEVLHQAKQKNIIQSIPKSIQVTDLNQTKILATLMEMQMCKLIDPKEIDPQLLIQFDTINFASQISNQSKLIPQFQFQQYQAQPKTNQIASSNVNPQSQNQAQPKTNQIALSNVNPQPQQFKIQNFSIQANPLQFMQKQQINKFQPISNFDNQLNQQNSRIQPNSLNLGYSKNIITNPFIPNSNGNLQNFNQIQPQQQLQINNQLHFKGNETIQQLENTAIQSVEQMITIGKFNFQINQQGDFVVYQCSSPEISGYFLQVKGKKGTLKDEKQFKWENKFGVQIADNLGKGFIIKWKNNTYNSLITYFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8D9</accession>
    <name>A0A8S1Y8D9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pectinacetylesterase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1560049</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000156">
        <property type="protein sequence ID" value="CAD8209810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y8D9"/>
    <dbReference type="OrthoDB" id="2015280at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004963">
        <property type="entry name" value="PAE/NOTUM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21562">
        <property type="entry name" value="NOTUM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21562:SF67">
        <property type="entry name" value="PECTIN ACETYLESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03283">
        <property type="entry name" value="PAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8209810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="406" mass="46462" checksum="AB51B67E620F8B48" modified="2022-10-12" version="1">MKKTICLILIVQSIMPFHLKYISDPKALCLDGSPSSFYKAEGYGTGAKKYILHFQGGSRIGGQTYDQLVKSAYTRSKTDLGSSKNLNQQMLYSGWFGRTKNGNEHYYNWNMIYLNYCDGTQHQGYKSDPIEYEGQKLYFRGDQIVKSWLSELYDDLSKAEIVIVSGCSAGGIAAYFWVDYIRSKLPASVVVYGVPDSGIFIDMEAIDGTNNPRQSLDLLIELVNSEVFHPNSECVSNNQQQKWKCYYAQYLLEYIKTPLFIVQSLYDYYSLSQMFKVDCANNYNLTQCTQDQLDFSQILYSKTYDVIMKRKQSFQETGGFAPSCIEHCFLLTSRYNSADWQVPGESGNTLSNTLNKWLNSNQKYDDNIYLDSVEWPENLKCSNSELSNYSNQFQIIIIILISSIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8N6</accession>
    <name>A0A8S1Y8N6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1540015</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000154">
        <property type="protein sequence ID" value="CAD8209257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y8N6"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="154"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="7"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="192"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8209257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="259" mass="31850" checksum="D0D70B0215C564AB" modified="2022-10-12" version="1">MSKMDPLLKLRQKQQRELQHLMEYEKQLRDKEIRNSQLLLKKKDKFQDISMKQLKFKEDLKQKAEQKRIEFEKNLSQAKMIELEQLKQSQLQVNKTIETVTKFENEYQKEQLDFLLKKKSEHNYQIEQVLENKKKIENKKLKELSQSLDAKFKSSQELQDNLNKQLSDRQKQEQQKQQTYFKQVEEQQQNLQKERMRKGEMFDEKLDKVEKLKQEKEKELKERQRLRLEAEKQKESLSKEFQEEIVKMQKQHFRKQIST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YIU6</accession>
    <name>A0A8S1YIU6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1770005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000177">
        <property type="protein sequence ID" value="CAD8213331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YIU6"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8213331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="348" mass="40923" checksum="8DF5337DC2A029C4" modified="2022-10-12" version="1">MGSSSSKKKKAKPQEDNLNKQPVQPIQAISFEFNTWEKSNQYIPGLQKKDTYNQNICNKNDLLFFQNNQQNKNLVDFNPINLNQNVYESPKFELNHHTNAYQFQQGLSFCKSEQNSIKQRIDQQKINKNLSNNGLKNMNREIQFERNQQQWNNNIPIINQRKELNLCDNQFQLYFPASNQQQLPLIQQRNDQVIKYNSLEINAITEKNIERDYIYIYILFFNKQTSETFQLIFPCKHSICNTIALMNVKGNVICKQGCASYPLDKSKFNKKTNTISHFQEIIFDDIKDYNQITSIQQMAKIFQDSLVYSNKTETEKFEFSKKFADLICKKSIRQAKQKAKQKIIGAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YNI4</accession>
    <name>A0A8S1YNI4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VIT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1950008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000195">
        <property type="protein sequence ID" value="CAD8214077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YNI4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013694">
        <property type="entry name" value="VIT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08487">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VIT" evidence="1">
        <location>
            <begin position="61"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8214077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="106" mass="12499" checksum="3389CE8FD92FF86B" modified="2022-10-12" version="1">MNKNKLYEPKIGKKEQKLKQIKKEYIKYFVFYDLTKKSIDTFEIDKIFIQDAQLNIEQSNRTGIVFSINENATISKIVVQLGEVKFYGVIKEKDNAKQEYKNNAIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YQL6</accession>
    <name>A0A8S1YQL6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1890001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000189">
        <property type="protein sequence ID" value="CAD8213874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF122">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN SKYWALKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="2">
        <location>
            <begin position="964"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="655"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="856"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1075"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8213874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1075" mass="127961" checksum="167FBD1B815F07AA" modified="2022-10-12" version="1" fragment="single">MIKEFFFIHLTQKNQNQPKEIDVRQVCLNQNGRICSIFFRLAGWKGKYLQEQNKYFYEYQKLNIKFQHSLLLYFFLLNYSWKNIGILIIQNKSIKNCFLKQTPIISHISKIIQSLLPKKINPLYTIIIISGFYQIQCISINYKFSSHYNSPFIFEFLQENLNQLNKQQYLMQTTLEIEKKLCQVHKLEIVAVDLKSQTRIDDKYLCTRCLAERVDKENMTLLNEATEMIQSMKTQSLKIAKEENVLRLTNLKQLQSSIKSFKNQIKTELGKLLHLVDQQMEQIQTEIESKDSKFEIKNYDDEIQILSKNYIGNFSYNIPKQQTYKEIDVALVQLIYESLQSQSKSQNFIQIMESIDQIRSSIPITQQAKINETPAKEFDQHKTPCLNQICNKHNKEIIMLNVNYNEPKFGRLACVECIQENPIQYISLKEANNMWNTLIGESEDLISKHNFKREKTFKMVIQEIKQLKNHYNNQLSEMLSSIDEQLIQSNQEFQDFLKLENKQIFELDEKQVEKMIDLLSQQDKNKHILQQQEKQDRLDYLFYQNVKSKLETLIKHDLLCKQQLMTIIQEQQDKKEIGNNPQADVKITPEIQEFISKCQLQEQYLQIHSESVEFQIELQKEVQQLEQKGQLEQYIELEGKNDTDNVKYTLLQQQFKQYEINSEKMRKLIKADENEKQLLLITKQKEELQLKIDEEKQEIKQIQQKIVDLEENMNQKFFKQEQEFVEQFNKETTQNNELKQNLLELTQSSEIRLEQLTTKYNELNQQIDDVQSELKEREKSSVVSDKLQSLTNSIETQIITVDQKVEKLTEKIKEVDTQQTTQTQLQKEELISKINIEINARDLLIEKQNQRMDQFIQKNDQQLASLSEQIKEENTAQLLQQKKLIELENQNKQQIQQLDDKIRNQLQNMNTVVQENTKDQKNHEDLLKHLVLQSKNDLLNLEKKNCFLQQIFQKNKFDQILKPNLLKADYWIKIFYILQERTKKNIKQPVLIYQGTRDGLNNQQYWKKVNGKENLLMVFKSKSDYIFGAYSPCKWESCNGKYIEDNTLSSFIFSQTHDQVYPLKQDQKQYAIYCX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YRK4</accession>
    <name>A0A8S1YRK4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T2140004</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000214">
        <property type="protein sequence ID" value="CAD8214434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YRK4"/>
    <dbReference type="OrthoDB" id="319481at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8214434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="63" mass="7604" checksum="EE079F569A9FF143" modified="2022-10-12" version="1">MFKAQLKGFKQSTVDEDKYLCMKCMLEKIDSQNIFSLEDTKIMIKEMRSEQLKKKRQRVLKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S8T8</accession>
    <name>A0A8S1S8T8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0050170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S8T8"/>
    <dbReference type="OrthoDB" id="298002at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="289"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8136478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="434" mass="52064" checksum="B3FA56A594822AFB" modified="2022-10-12" version="1">MSARTQYNEDIQKQLIEVVETYGGKNCKSWTQVARIFELTTGIKVQNTFDLKKKWEMFTNYKEELSQDELKALYSTGVRCRGRHKAGNEEFFKLTGKKLYVNQYSKMVGNFLLQAIKLLCQSFFNQIKVKYRQLGQERFHSRISYVSIHILLRAVEINKHLDDFFVQELKQSAEKFEQLLLIQAEHHEDERIDRFQHYRRIINRREFKKIAFFLDYVNELKQISINLYQEQIGRHPLLFPQIEENKSSKIYQLLIWENDWQSKYNLFQECAAKSDWEEYSMLIKKTKKNEDNKQIKKKTPQQQVQQKQQSYEIINQETIEPETNKLKQNSYDLISTATHKVCNNASKQQIKQMDKQELKSFRGHYYREGQIRTKGLTTILFDTDMRSDEDEADQYQNIEENRKEFFDKMEELYDYVFGGKANLVSFEKSQPIPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SCX4</accession>
    <name>A0A8S1SCX4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">VTT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0050408</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TMEM41 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SCX4"/>
    <dbReference type="OrthoDB" id="3364966at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045014">
        <property type="entry name" value="TM41A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="VTT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43220:SF18">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 41B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="VTT_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="VTT" evidence="7">
        <location>
            <begin position="97"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8136912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="266" mass="30379" checksum="F9F21A79313A17DB" modified="2022-10-12" version="1">MSTEIDINKLKAEIKNDKQRRLILFGIFATGLIGLYVLVHFSPHLTQEERKIVYRIPKYPHHIAELLNVINRYTENNQFYVLFAFIYLYIFMQSFAIPGPVFLSLLSGQLFGPIPAFLLVCLCATTGASLCYGLSYSLARGLVLNRFPNQIVNFNKKIHKNRDNLFFYMLFLRFTPLIPNVSINVSGPIVGLPFKYFFFGTLFGLMPGNIIHIRTGLLIQELNDFSTSYQALLTLIGLSLVALLPTLFKKKLQELDEKGLNKSKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SCX8</accession>
    <name>A0A8S1SCX8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0060118</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SCX8"/>
    <dbReference type="OrthoDB" id="285465at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8137490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="140" mass="16288" checksum="9FA427E14886274E" modified="2022-10-12" version="1">MQQAIPSLTPEIKYRLEEQGFQPFKYRPLPDYANPHTLSYWLTNAGLGALCLIGRHYACQQQSIRILWSTAAIAIPLYAIATNAKLDGLRQNNFYRKTLDDRLELHPLTRRAWERAKETNKGYQDQLREQIATLEAELRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SEZ2</accession>
    <name>A0A8S1SEZ2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0060199</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SEZ2"/>
    <dbReference type="OrthoDB" id="421226at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="300"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="342"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="407"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="448"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="479"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="7">
        <location>
            <begin position="586"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="63"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="111"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="789"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="913"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1030"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="77"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="113"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="827"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="866"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="913"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="925"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1038"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1070"/>
            <end position="1085"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8137652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1145" mass="134099" checksum="B0C4689039D820EB" modified="2022-10-12" version="1">MNLQPVSSGSINMSGDSNQNGLDHYLNELNNQNLMQVRRPNEEQILAFIDFEGIKPDQAIQHKTFPDSHKENKSKGSKSSQQIRRNSLDLSEVSPLKQSLRDDLIPCDSPNIELKKLGDPDDHRKSKTVISPAIQSSKSPKRKSKHFSKIFSKITNRMHLAKKFIQKIKLLSPFAKKIQKQQLQLIQDLSSDINNCNEKYNNNSFIRIHDKSKQKKVLKRIESMKAFIALKTYFSVCKIQVIFDKLKNETIDPQGNFLFIWELLKFFMTLASLFQLSIQICFNLDVLNWFFISEKENSQIILIMMFIYYSFDIVLGFRTGYYENGEVVTKQQRVARRYLRKYFFVDLISIIPIFVNIILIQSFETDNTIIKIVNCLSFLRSNALNRVYHSLEGRLLSNPRFVIGYRFLSVIGTVFLYAHVFGCLWYVVAQNNQNNWINKAGIVEDSWFALYSYSIYWSVMTMTTVGYGDLTPANHEEALFCVCTMFIASVVFAYSINTIGMIITEMNKFDEKINENMAIINRYMQRKNFEQSLQFRVRQYLQNLWTQEEKFRIVDENKIINCLSPNLKEEIQICLYGQFITSIHIFYRYFSQECLLDLTKSVQEYRVAPNSYVIENGTHDGIALYQLVSGEAEMFVDLQDRKYYIGKMKQGDIFGHGPFFMNNVHPYSIKTDTACSFSYLSKQMFLDILQSHPGDYQTYRMIIDQWTFQNQKLDLGVKCLGCGETDHDIDQCHRLHLIINKQILLTKHLFSVPNKRILFHRNSRRTTNAKFGQRLFEDAVSLFQENNYSFSSEEEEPNPSQIERTHQTKQTQDIRYQEPTHNTHRSQQQQQQQQQQHQQSKNVRFRKRSRSLNSISVHSDEESKFQKSISGYKSNDSQIDKISGIPVKVQQLPSSQLSQSSFNVFLKEYPKRKISKQQDSDKYSNHSIGQRQQQQQQQQQQQHYQQQQYQHKSSSQSSSGNIPQSSIGISPIPTGSPPLLYQEDKQTFQKGVVEDSLFVAQERKKKSLIGTSYTNTKSIKNPLQAIPSVSEMNETEKNNNNLKEGSYTSKQLSMQSRSRSQRYTISIRTESQKIREDTKHDTTQKHDFKIKTDTYKQYHENSPLNEEQFHNTFEQACNLNSYYPQYNKDRIIEKYKRQQYRKNNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SF46</accession>
    <name>A0A8S1SF46_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0070121</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SF46"/>
    <dbReference type="OrthoDB" id="289994at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8138450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="313" mass="37561" checksum="63CBEF05B589E5AE" modified="2022-10-12" version="1">MSSIYSEHSTPKCKSKQEANLDKIHHKTITKQKKLTLQIKKKSEQSIILAYQNNRKLKQQTYEITHTKYLDRSSGSIQNNLIPLGRLHIDEFTINNKILERKITPEFIECKLSPTNIQEEENGLQKNNSFYQYELLQLYNSLPVHAQQQNERKRYEELLNLPYECITLSFHNNKLIKIQKKWNYDFLRMMGINQEIIDDYIFNHNLLPKCWDLNKIFQINGETRFATNIKNYQGEEFICHFEIKIFTEQNKSTSIKEQVIYFSFQCERRFLSVEKLQTNFQHYFKLDQITSDWQMSIHRSKIVKRCSIRTKAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJI6</accession>
    <name>A0A8S1SJI6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0070385</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJI6"/>
    <dbReference type="OrthoDB" id="292925at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="43"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="94"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8138932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="222" mass="26593" checksum="C759363F6204A5D8" modified="2022-10-12" version="1">MATFKTLMKMPQAKLASKCIALEELLQRYYQFEEVHYDLLNKYQLLQTKYERLNQQNIQLMQNLHIERDNFSQASATKMNSSKNCVSRILKDPLEEMENLLLLEELKNENQKIKEELQINKEELKRLQSKFEQTQTELLRSASIKTASDSKLNSIDERNYDSIQSYKNEIIRLNKIVSSLRIEISHYKTREFDEDWLKIQLIKYQKQSQMSNNHNFLDFIEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJQ7</accession>
    <name>A0A8S1SJQ7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Acetyl-CoA transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0080451</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8140098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJQ7"/>
    <dbReference type="OrthoDB" id="6415790at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008521">
        <property type="term" value="F:acetyl-CoA transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035348">
        <property type="term" value="P:acetyl-CoA transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024371">
        <property type="entry name" value="AcetylCoA_trans_1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004752">
        <property type="entry name" value="AmpG_permease/AT-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778:SF9">
        <property type="entry name" value="ACETYL-COENZYME A TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 33 ACETYL-COA TRANSPORTER -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13000">
        <property type="entry name" value="Acatn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="14"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="213"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="277"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="307"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="372"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8140098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="416" mass="47745" checksum="61A7597B8FB37221" modified="2022-10-12" version="1">MMILLEHGVPYSNLAILSLAIYPFSFKMLTAPFLDVYYIKSIGKRRTYIIPIQYLMAIIYTLLYFTKISTWVYNIEFLTLIGFILILLSAHQDIAIDGWVLTAFSSEHNHLGATAQTVGQMIGVIFSTTIFITLNSKDFCNSYIYTIPQETGILSLDLFCLINALYLIALTLYVQFCTTEQSKEESIDEIEQHQIKTVFRNLKELVLNRNLQFLFLHLILFRLCFQPILTSTSSLLIAQGLKKEVMAWIQTIMIPINFIIIALIGKYDKRGNELQKFFHYLIFRIGEAILTYSIFKLFPDILSYESIYQYLYLIFFGITQSLLSNSIFINHGTLFNRISDPQVGATSLTLINSIHNLGGAITESISILSLSLLSYDIVALLTVCFGLSYYFILKKPLIKLTEQNQKVWQIKKKQSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJU7</accession>
    <name>A0A8S1SJU7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0080471</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8140138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJU7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8140138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="139" mass="16243" checksum="CBBE038422BA8862" modified="2022-10-12" version="1">MIDTVKQSIKNKENTQAGLIIEGDEYLKSSQLLQYTQNNNESINLAGKQLTNFQQRLIEKISLYNKPIEVALYQVPEKICKEDVHIAFKQYHIRNCYFKRLNCFFNLPKDQANQLVQETNLRITIKGENLLIVMLPLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SL39</accession>
    <name>A0A8S1SL39_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alpha-type protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0100072</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SL39"/>
    <dbReference type="OrthoDB" id="430683at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04515">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004166">
        <property type="entry name" value="a-kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052969">
        <property type="entry name" value="Thr-specific_kinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47763">
        <property type="entry name" value="ALPHA-PROTEIN KINASE VWKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47763:SF1">
        <property type="entry name" value="DUF659 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02816">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00811">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51158">
        <property type="entry name" value="ALPHA_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Alpha-type protein kinase" evidence="6">
        <location>
            <begin position="759"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="159"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="17"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8141355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="995" mass="116390" checksum="D5344973B5CA9CCA" modified="2022-10-12" version="1">MFNNNQQPFQGQQQNPTSSKSSEIQPKPFPNSQQNQIQQGKTFYPNQSVVSQQGQQITGNSKSLITITPTSQNSQFQLQPPQQIYPNPQIQQVVQAPSNLNNQSNMSNQQIQQPQQIKSQVVPPMIFPNLQPPLNQIPRLDFIQVQGNQNIQGNQNIELIQRKQQLNELEKKFQNLSQQKYEFDQKIKSLNQQKTKCQVLIQKLKDKIEEEEKKKNNLSQQLENQQKKLDSELQELQNEFDKIQVERNELQTQLGMEDQKINQLIQEFRDSKRNTNIDQNKQLQSKIEQLESENRKLKEELEVQQENQLNNQTKDNEIQKISEIIERKKKYLQQIQTELKSVNDDIEQQESHIQQRDQNINQMSFDLKQEEKKLKNLVQQVDSMKEEIKLNDDRIRLKTEQINQLEQKTLSALRNSVLVQQISNLKKDVEHQGLIYDQEIKRQQGDLTKSKIMMGNNKKEQKQFEESQRLLRIKQGKELQMKFKDSFGQLEVCFLIDSTASMEPYKLQAQQCVKGSVKAIKDLTQRDVNWSTVCYVDTDILQRLGGMYKQFGFTRDSDELENFIQSVQCTANRDLPEDVEGGMKQMLNNIPWQTKFKLVILICDCPCHGTKYHNYGRDGDYQPDSDITPTIENMIQQEIFFIGIIFTNETVKMYEEIRKIYQRNNKEEYFILSDLRQVPRDQQFQKLTEVLSFASQQATQTNVRTTRTINQTNVIKQTKNQKDNSEDYSSVEALCKVNLQQGLNADNAIRETFKVFRLQMKEESFQQKMKMIQNIGIDDFQLIEENDWDCLRSKNAFAKGAMKAAFLMTKSKFMNSKNEFYVCKTPLNMQPYPNKESALKECILHLVSQKWLRKFYKELEGVTEQITKQIPQLSYSDILLLQDANNKYWLAERFFEGSFVKYNNNWGFVNRSTDEVNKIAQCFSYYTYFKSDYQYMICDIQGVGACLTDPAVCTKNNYKIDPTDTGEEGIGRNIVSFGAVKNMCTNILNALKIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SUS6</accession>
    <name>A0A8S1SUS6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0140214</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SUS6"/>
    <dbReference type="OrthoDB" id="10265007at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030865">
        <property type="term" value="P:cortical cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035303">
        <property type="term" value="P:regulation of dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21505">
        <property type="entry name" value="PPP2R3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000091">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.220:FF:000002">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041534">
        <property type="entry name" value="EF-hand_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039865">
        <property type="entry name" value="PPP2R3C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12085">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B'' SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12085:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B'' SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17958">
        <property type="entry name" value="EF-hand_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PP2A regulatory subunit B'' EF-hand" evidence="9">
        <location>
            <begin position="181"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8145381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="448" mass="53392" checksum="714B16652EFAA0A6" modified="2022-10-12" version="1">MSKKGIDWVSTLKKFVDSKQQRTAQEIDDETDQLFLDYIEQARKKEQEEDPSYKKIPKFFQKASVTNENQLSFRVRQEARTRFLNHKTGEILDKEDLEKLWTELKNNISPPDDKKERINYNSFLTIASLLPIKCRHFFSASTFLKFDRDEYGRIDIVAFFHSIVRKVNLFQTRIQISLYDSIGNGYLREKDLENYIFELIPTFPQLEKLDQNFYPFYVITAVRKFFFFLDSKRTGRIYIKDMLTSPILAELYELRQEKLTLEELGQNWFSKQSALKVYERYLKLDNDHNGLLSKQELSKYSWGLTDIFIDRVFEEYQTFEGEMDYKTFLDFVLAMENKKSQQAIQYFWRILNVYHKPAIDSFVINMFFRPIIQKLEHKDKFGFNVEDVKDEIFDMAKPAISTAITLTDLQNCGQGDIIISMLIDAKAFYEYDQRESGQLLEVEDYEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SXB4</accession>
    <name>A0A8S1SXB4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0120202</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SXB4"/>
    <dbReference type="OrthoDB" id="306940at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10476">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="185"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="38"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8143384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="209" mass="24386" checksum="BA1F67B4775D3C5E" modified="2022-10-12" version="1">MGQPNQKAAPVKARDPMDIQLDLRMTQKRLESESKRSLKEQDKNIAKAKEALKKNNEEGAKLFLQTAMAKKQESENLQKMATRIQFVESQVKSSQANADMMQQLNVVTPIMQQYANTMSVEQLYQNINQFDRALDDIMVQGKVVDSMMNKNSNDFQTNMAVDQMMQQLKKEEALKLQELQPSNMQFQQLQQQQQQPQYQNQQQYQQQKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T0P1</accession>
    <name>A0A8S1T0P1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0140048</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Organophosphate:Pi antiporter (OPA) (TC 2.A.1.4) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T0P1"/>
    <dbReference type="OrthoDB" id="3639251at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000849">
        <property type="entry name" value="Sugar_P_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43184">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY TRANSPORTER 16, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43184:SF12">
        <property type="entry name" value="SUGAR PHOSPHATE EXCHANGER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002808">
        <property type="entry name" value="Hexose_phosphate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="119"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="295"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="328"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="359"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="384"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="426"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="454"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8145047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="492" mass="54943" checksum="07FF4A293FB6C5F0" modified="2022-10-12" version="1">MFDSAVPGLRAKRIQVFICCFMQYAMLHSCRSTWSFTSGILAKQDDHEHDDEKIDDFDKKYLGYVNFSFLFVYGSSMLFLGQIGDRIGPKLFMLCGTFSTGIIQFMIAALLPVEKHNRWLLLVLQIFNGISQAPAWPGLMAIMNNWQSKPNKMIVMGYFAACTNVGNILGDLFAALLIEQLGMSVMAPIYVSAVGVLIMSVINIFLIEASSAKEYIAQFKNPLTKSLVYKQAHDEYMSRAASVVSVSQESFITDLSQTLLTVDRDSQMNPHVPPKPKKNKKDSINMISAWKLPNVALYAFAFGCIKAVFYILAFWLPSYLKGENLEGVAWITQMIEWGTIPGGILICYAGVYWNLRASLIVPSLWIGTVVMVSINFLSKLEQPYALYYILVFLTGLMIGGVYNNVSGAIVIELSNMKELKGNKKSTATVTSVVMGYGAVFAAINQLIVPYLESILFLYCSLNAIVAGCFLIPLIINEYKRYKEQKIQVSDTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T412</accession>
    <name>A0A8S1T412_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0140235</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T412"/>
    <dbReference type="OrthoDB" id="285940at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8145422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="331" mass="39111" checksum="967796E090693DDC" modified="2022-10-12" version="1">MFNSYQYDKQLTFTKEIDKIKNDYLKQRENRKELNQRLSKNYKKRIENFVINMLEKPVVCNEYKPPEAYQFRDEDPTKNLGDPQIYVKGFKHEKDRIQEAQEKNNNLDFLPNLKVGKYCFRERDPSKDIKRDVFRYRDKTALARIEQFLKDHTQSQVENMKLDHKKILNLEHFSEGMSSLERKAYLSRLIAKNLLPSLHNKTHFQAAQTMYNNLPLTLMEHARSLPQVHTQEDSRRKPPTSQEKQKTIPNDNQNKQDDNGTQPKQGNETETFNPIETSKQILKKCNIIKERNNKAPVVHSGSGHLISTLDKSISEIYKELYKVEIGQSKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T8V8</accession>
    <name>A0A8S1T8V8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0180213</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T8V8"/>
    <dbReference type="OrthoDB" id="306314at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="101"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8148733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="278" mass="33141" checksum="B8A17DAD8C06583C" modified="2022-10-12" version="1">MKQKEIKLVYEKTNEVYQWQLMPLDQLIQEVSQVFNIQLGTFQVVSQDKRIEIRNTEELYWYGQSGGILITEKQTFQNRLYSPVPNLNLSQIQNQKQYSIVRKTQEIKKRLNQTIKLMKNTQSTVTYRSLSPKESWKCLQEKTKQLQEQTQQFNSRQEELQQESENLLKEILAVQNENVMYSKRNDGLQKSINQLQMLLKLQKKQINQIKMRYPAIDLDSYVNSIKYQSNLKDTLLNYLQVLLQMILKGEDKNNFKTTFNQNETILCLQQIMKEIKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TB11</accession>
    <name>A0A8S1TB11_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0180251</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TB11"/>
    <dbReference type="OrthoDB" id="8192126at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00024">
        <property type="entry name" value="CD_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="2">
        <location>
            <begin position="15"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="291"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8148807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="337" mass="39868" checksum="580C5948203DAB01" modified="2022-10-12" version="1">MRPTKQQDYQLITPRSPERILKKRILNNQSQYLMKWKGSEQTTWEYEENVRRSILEEFLQNQQSFQEQKQKNLKNTASQLPVLQQVQQSLKHPSYSEELLLNKQKEMAQKFCCSYPQKCDEIDSVHLMLTNEDCVFEVKWKPRADGVVPISDFYQYDQFKLAAPLLFMNFLENCIVGHENNTDIKFYAPGKDNVERAQLIKRILLRNPKYTDSNKQHNYVVIEEQKQQDSPQVLQLNKNTIINFTQSDTKKIIVNQQTESLDKNQCPQQVSEKKNTIIIQESYEIHQQQLDSNSQQEIQQQQQSIDEREIGELVDPDENQLNDQQLEQRSISLEPQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TBL5</accession>
    <name>A0A8S1TBL5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0200362</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TBL5"/>
    <dbReference type="OrthoDB" id="290206at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="316"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="361"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="411"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="446"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8150595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="539" mass="63446" checksum="5BF49653BFF75FB3" modified="2022-10-12" version="1">MNFDTNIYKSSLMSKWILILLLVICASLVAVFTGIAEVSPQILTFTDVVDDTKCQNELNIQQIQGTQEEFKSVLNNNLLCFFCHENIPDQPFGIKGILLNQENIVTQTMLTYSIGYFDDITKRPCWYDASQNENINHLPKIKSTIYDKQIEVQQSTILLNWECVKNITSYTCYTKEHIYKDNRSFSRLFGFVMIEGIQYQYMNFAIMKQSKDYYLYNEVMMIAWIGIELVFCCILFMLEDEFEEQQAKVLIPTLSVRNQIFLIFFLNNHPLFLIQLFYDNLYLKLFNVIFQSIGQAGFMFYCISITFKLLGLNQQFIAIINSGICVTYSALKSFITIVTGLDMMKNIPQSSVYLNQSNFTFIGLFMVFWYTYFIVVQGVLFIKNRQNKAIQYKYARTDINMKLCHKYFKGIFIFQIFSLIFISAFFARILWQNIVIFNPNYLYYDHYQITLINVWVVSLFILFHLENPNKINQILEVDEEEISDKVEIQLCNTIQLEEEQVKLQEIPEQNLEMGSKTLDTNLRKVKYNDIAQPETQLRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TED4</accession>
    <name>A0A8S1TED4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0190026</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TED4"/>
    <dbReference type="OrthoDB" id="291390at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8149272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="264" mass="30466" checksum="7E090EB486B40949" modified="2022-10-12" version="1">MLSPEQIYSIVYKTDSGIEGYAIPRFYHDPAEMMRNRDLLKQIEKKQAHKRYETKKGSYLDDYTKMFKSYPSPLQYQSYSIQKVPDKNVKYPNRLTLFDQLQKEQKRINNPPVGAYNIEKTQEQIEKELKVLKTRKVKALQRPDPYEDAMAHSIMVPGPGNYNPHSFLPKIKVNNTKPEDWRKKHNIEKNKSLPQLPPVGTYTPILSDSFAKIQCTAPVKRSYWGSEKRFKGLFGSSSIGPGPGTYAQLSKLIAKGVEKSIYQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TFX6</accession>
    <name>A0A8S1TFX6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0210026</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8150808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TFX6"/>
    <dbReference type="OrthoDB" id="306912at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8150808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="513" mass="60733" checksum="807EAF598B1359E8" modified="2022-10-12" version="1">MLSNEYLTKVAQLLKMTSHFETQLEEQRIKLSITPHFQARTIYNLLITNTKGIMRCDIINLFRNFMQNINERELDCLFQLYSSDNSYINWDDFYQLICPYDMFLDTKIHDNINEQIVSDILQQLQRLFKIEVMYFRQAEPIRMILFEKYKENGKQFCTLLEDKQKFISTQNLINFMNKQKFQYNNQDIFCLKKRIKCSEENIPSELFIKYCPLLPFKVMISKSPDYRLITIRQPAPEITKSAELLKSKPIEQQVLPDIPQINLVPAPSQEEETFQQVASVPQFKQLKKAEISNYEFYTGKKPDATLTQSNFKQNKPENQNFKNQNDYDFKLTKSESHNFFGFKQSIPKDFGSTQRPEISKDNDQILDKYLRPSASRKGELPKKLFNKEHNNYQIQKKKSEDFQVIENKNKTGYIQETQNKPTEDILNEHLHPIKQQKDQFFDSGALTHTRKYKIFDDIPNNQLQLQSNMLFDSKSKIIDSAFDSGNLNSQQIQKKSKKYVTASMSQEEKFPQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-06-18" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TJV4</accession>
    <name>A0A8S1TJV4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0210347</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000007">
        <property type="entry name" value="40S ribosomal protein S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_uS12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00055">
        <property type="entry name" value="RIBOSOMAL_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="304"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="137"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8151426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="341" mass="39168" checksum="9337AB1241D7DFA7" modified="2022-10-12" version="1">MGVGKPRGMRAGRKLKDHRRVNRWADKEYNKAMIGSRYKNPFMGASHAKGLVVEKIGVESKQPNSAVRKCVRVLLKKNGKKISAFVPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXWINELMQANKEIRNQEQQMRIQNKQLLEDLESWKSRYKLLQESNSKSSGLDEEIRQLKKKINELSEDLAGSHEQIQQRDNEILNLKQLLHDKDNELDQLDARIRELEMMCENYSQSETQIISLQGEVELWKKKFKQVNEQNSDLAEKLTMAETQLEAIKKRQVTVTKETEVRKSGTHGGGTTSTYEQNVIKGAQLLHPRGSQYLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TJZ3</accession>
    <name>A0A8S1TJZ3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0210367</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TJZ3"/>
    <dbReference type="OrthoDB" id="10252354at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="11"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="77"/>
            <end position="79"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="9">
        <location>
            <position position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8151466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="529" mass="61672" checksum="549DF5153634183B" modified="2022-10-12" version="1">MKHDFIKERDFKIIEKIGEGAFGQVYKGLYKGEEVAIKSMQGAQLQETSIMENLKHKNIIKFYKYFKQGNNQYLVMEYAGGGSLSDYMKQNLDEETISKIMKSIFAAIEYLHSQQIIHRDIKPDNILIQNSEDLSSVKIADFGLSYQYMPEIRYYQTVSQQCGTFIFMAPEQILNKAYNKAVDMWSCGVVLYMLLNQGKHPFYPRISTKKEFINSFPDLKYEQPLHASLLARDLLQRLLQYDKDSRYTAAQALVHPWITRNFSQSIPMSVKEFGICQEQKKKFFQFLKAILFLNQIQQDSIKQSPIQSKTDLGDFKISIVEFDEDNYEQEYQDTNSPKNQLFLNKLSIQRPNKILKSYRTLNNIPYKSKHYQLNSILNQTNESPIRDLKKLKFQQQFSIDNEQMIIPIDPESPKKYLRGTQSNKFLLPALTESTQISLSPIKQKQKPHIKQNSFRKSETSFFMGLNRLNNDSALETGTFRNRVETYDRTQIPVINTQRFYGRNTFTNLVTSPKNSIASSTNLLIPKKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TMY9</accession>
    <name>A0A8S1TMY9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0240152</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TMY9"/>
    <dbReference type="OrthoDB" id="308602at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="442"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="606"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="447"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8153333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="628" mass="74572" checksum="F2455D11CE082B53" modified="2022-10-12" version="1">MVSDFKKKVDAFLKKNNQKSNLMSNTKIASSLKELELLQQIKNEELLNKSLCSNKYTVKTEKKSMKAMEIFILGNNKVVTKQVISSELIQSRLLKQMETFLKDDRFNYDLKMTTMIEQYAEIVNRIHEKAFTIKIPEKYIIQMSDYNEYYQIKEDTIENFLENKLEIIADQIIQEIQQSKRTTPKIMSHKTSEIYEPIEQPTTIQQIEDLIKTEPNIVHQQEIIKQDSPKIEQIIPDRKVSNKKSRQRKKNQQTKNDKNLSEQSSLSEIMDSSLKNSNIKDNDDIRRRRKEKSKITDQAQIEEEEQDLIQKEIEKQKKQKIIKKLREFNQLFKPQMYSRRQSEDTMLQIEEPILLAQPKQKVLKTLSLRPADDTIIMKEVYEIIRVRKQQLYEYQSEQRRKQEQALENSIQNQNKIQQKPLNLIVNGKHISELKEAAELWDSKRNSPRSYTKQRQNQNNSTVVEQKQEEINVNEQMNNMMEEQNNQTNSQNMQDNNPDNSINYQQIINEKLISLEEKLKQQCQDIFDILLASEFVYDVKIDDYKIGKPHSNKINHPNFIFQQEQDEIVFTHQVLWRREIQQVIPKLNSVNKQMSVLINSEDEYVQDSQQIISEEEDEDKSDQNDDSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TT09</accession>
    <name>A0A8S1TT09_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0250195</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8154109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TT09"/>
    <dbReference type="OrthoDB" id="77931at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8154109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="594" mass="68368" checksum="70E6395673032E86" modified="2022-10-12" version="1">MNFQIPKSSDSGMIVVSNSKIKIDSVSITGSYSVTIFSLTFIHHLSITNVKINKAKQFGSPFIYISNEQKYQLDQKIQSQLFLSSININNCQNMNQQMQGSFLDIILIAQITSKIKLENFQIIQNNCSNCQKGVINIQFTEYTKSINIDQLLFYQNNCGFQSCLSATPIGTYKRTQIKVDHALFIQNNGSMNGTLNLQASQLNLQHIKFINNTASNGGGYYSQYYQELETKDIYFIENKADIGGAIYLNSTKLQSSSFSQIQFLGNKGKLAIDNLQELPIYIMLSIFQTDIETITVGGQDQPNLKKFLNESFIYLPSGQKIGQYQLFSKKVNNYQNYNIQLKFYLVNNLEERIFQFDNETKSCIVKQKQFIGEQQQTVKYNDLIVDYDQEDQSFIFENLTIIFDPYSSQDSYLNLQMICQIQSNDIIEYQLNVKTFPCQVGEYYYESQCLLCESSKGYYSLQPKAFYCLKIDPKMILKNTKNQIELYPSYWRPFSKSSLISICIRKPETCLGGWETGDDSCQVGSIGGLCEECDIYNIRGFGQYYQNNNFKCQYCSNFIGKTAISIVITILQFLYLNLYLEHYYLLIQLLIVLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TUK8</accession>
    <name>A0A8S1TUK8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0270275</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TUK8"/>
    <dbReference type="OrthoDB" id="307293at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="38"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="183"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="271"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8155753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="354" mass="42830" checksum="6208661B938F7826" modified="2022-10-12" version="1">MQISPLKKKINISLNTSRDARTSLQDVITNQTEQIVKSNQLEQELHRVNQQRKLLNMELDDLSNKYVEKSQALQECELENALLRRQIQAYRQQEDISKLHILLQQKNHIILEWTKKFEIMEKRLENESKLEQQVKQQQFQNDILKQQINELKQQQLISSKNYIELKEERNQYYRKSIDLFQAFQKLDNQYKQLEKDRNQIQSELIQFKQDCQIFKEQLNQVESILKKKNKWKLRYQDLIKESQQNQIENSSESMVEQLKAEVQIGYNEKELNLIENNNNEELNDMKDKLQQLEQENQYLREQNIELQNQFQSATDKLLKQQLQSSMIKEQSQTFSFVKYHHEYCDSLEEQQIQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TVL5</accession>
    <name>A0A8S1TVL5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HECT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0280188</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8156208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="290242at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043366">
        <property type="entry name" value="HECTD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46435">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HECTD4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46435:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HECTD4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="4">
        <location>
            <begin position="3516"/>
            <end position="3858"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="388"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2204"/>
            <end position="2227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="3868"/>
            <end position="3896"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="3159"/>
            <end position="3264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="2204"/>
            <end position="2214"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="1">
        <location>
            <position position="3834"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8156208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="3896" mass="455628" checksum="40E5B88BA6B3465C" modified="2022-10-12" version="1">MSFPQHIPSTSLSFLDPSLLIGNLDWLYLLSMKETDGLSEKSLTKSEDVSGFDAFLEQVDFTRSAYIKLRQDYFNLIQHSSRNLTESLLNSKNEYFSRASMMLNKINKSKIEKPIVMAQYHECSQEVFKQCQGVTSCSALQSNPTRWPWGLEDFHKSLCKQELLSLFYEPEYSRQIIDKNLQRLVKEIDHKQLSKILIDYALRVGTFKQILSLCDLNIREIIKLPRLQQKSHHLHYMRSLCDQGGFTITQLQQFTKLNYHQMTMTSSQCYLYILVSSPVGGMYKVGTGEGGTIAGKIYLHQPIKKQDDVQWVFLQNKLYLKQQNGTLDIICPETFQTLSQMTLHCPELFQHPQMQAINKNYPLVTDGEKLFIIGRKLNITKIEGAPAKVTKEPAASNPRQQKKKEAEHQPADNTIKIMDFILFEFDLNKPTGNVQNEEESDKKLLAELYESFQGYFSKAEISKALNLNKNDMQNAAQWLVEEGEKERNKTTVSVSKQTLLCQAEITTDISAKQVKSSADIGCKEGSILQPQIISNIIWTMDKKYLTAYFEFGIKIFSKDPADEKELDQTKDEFNLRDHYSTKKPATSTLHLKGTYITTVNKYYNEFYQNPNYIVSYEHSTKRFYVMHIQWNSTDLQFPALVTIGSDFIHRPLNLNLSQATTFDEEVVNFLLQQQEQRYDQEWRLNDWYVAYQLMLKNLRSISEHFSEEEIKKIQSEKSKYVHRLNKSIQSINHTRTEVEIKKVYAFCVEGTFEELKQIWSLFKKFHKIQYLQLLQYWVQYSEISILLNVGIYDLLKEIGEYLIQNWQNDLHRNIFIKGWPLFLCSNQLQLQYLRLGDNQLLQNIISTYSSIQPETESAILKLKQKNINLDGDFKSFIHKKGAQHFLIEPLPRLLVEVVQNVKLFQKVYQKGYFVDCDISQINQQFWILAKEWAIQCDLIKWQFLTMLFIMAFNENNEWEVQYKLAFNILQVFELGIANKDVKELIIKHNFLTHFLLQLFLSPVLQCPIDCSLLSVLMQINQNIELPTIPSRSQGIDPIGYKVFETSHPYERQKVQTFEDTYFPGALALAVEFDPRCSSDQAHDFLTINSWYSPQTGPFGLQTKLKDPMGVSYRISGKLSTKRPILMLGNVIQADFSPSGQVRNEHSLNRWGFKITIRPVYGDTYHVSNVLHMVILENIQSLLGFNENRTQYEENNQHLLKWNILKNGRAGFQYDKHLIKVLGQLSISNMSYDDATLLHENAGANPQIKNKYQQLKRDLIKVNYKSRDIQKSNINLLQLAQQQNKYISQILDKKGEFYKVISDIVSQFTDSIQYRTAKQKSAMKAHIEEFKQIEMLVILVTLYHSGLLDEINLLNLEHHIDMLQEARNDVINYMLKEVETMIEYQQNYEGMSLQATEMMEQQKLKQINEQIENTLWDMLQKRLEGNSDMIYKICTQQNLPIIENNPNLSLRQIYNNILAKIRESKLDLVNPYQHIVQVIKKRILILFNISNVQLVGQQQMIQIKSQQSVDSGSIKQLKPLGIERSISHQIDDKNIDSQKLLKFRQWLDSYQKWKVLQNVNLNQISAESLDIPPVKSVILFLQQNVDESLIQVMDLHNRRCIRRIASLKFLNEFQGQSGGILCQDVMNCKIEFASSNIKEILLQEVVKRLNYLMQSIKNDYATYLNQAITQQIDGTLLEQLKVLCMTLCEINQLFQVDNNILWKSREHDYNPHFNPIFKQFLNNIIITGLLSLAFVNLDQQYQSVAILSQNILNNVKETLYIIADNLMTDDILQQLIQILSQELGISLQGDEINCRLLTKLYDLIGVKRVNFLLTNILDCLIIHQNQDVPSSLVKLIFFILHHTNTPSTMRLAIRISKYFNNINLNKIKYNPKYLWIYYENCNLQQIKVLQENKELYFSEALIERVGHLVGQNEDTNYQNINIILQNELTNVQKKMINSQNTNCDQNNNQQYRVILHLCQEEEISILLKILYWWEELYPSLKLKLPQTYQEYLEMKEKEKKREEQKTQQQEVIAQQTEFQSQRQQQQQQYKLLERVLMTQPEIQQKDYLLGWGFNYSNLYSDMKTGPSDNKKHSSGRVKKQIKKYWRPLNFQELERLYEELYKQNEDIKQFPDEQAFLRQRNFITRIHSHIKEIKNYGSLLTVYGCIAFSEIFNLQKAQVLVELIQSALNGTLPNIPMNDLSEEFKNKADPNKYILLGAIKEIAPPKSKSQPQKAPTQIPPQPTSGKSQMTVSVCDVYTFQTFEFRMDYITLLPYGNTSVNFYNEINRSSSFTQQLVNGQIVGQLIYELSQLLCHQQIDINKQSNDYKLGYTTILSQQLIQIAPGIPVQIKKHKSYDKQYVLSGGDYCGQKELRLLSNNDDKLNLQVVKLDEIELIQQDIPQQITQQQILELIEKENDKFILRNLFIIASKLEWKGQNVDKIINKILNLANLYQGDNKFIQAWERLIDSRLQPNYFYPIEIPKSIKFLQQPQQVEQEDYKIEPFVENVDNTIIVGSTYMQSLPEACIVSNELKLVQYWEKNILPKIYDYVKGSLRPYEIDDFFEQIRQQLRKGEQHKALEIAYIVCDQRMPNGVVIPESNHDWSTLTIEEVQAGQYVLCRLIDKTSEQLYSKEYLHYKKQGQTFICGQIIGVDQRSSSILITHMDINNSQLYATWFPVSSLKQVQLYIPPNANAYQQETILKEYNRELELELSKQARQLYILLSQTINVDPITYLQLAMQFEGSCINGWLTEQLIDENTKTNQKLNQFMQNPDLIFGWLSKSFDQIQQFVNNNKLLLDLVKSHKKPLELNSDLDIAGLAIVFKNDATLCTCSGINFYKDRLGINDFYTISSGKETRGRLSPIVLNQKTVYCEYYFNGEALPIYQQKLCVSRLPCVVYKIPTLWNTVCYVIDQMSTIFIKNKDIPNLKNINHLLEQVYQSQPHILNQSIIKLLIRNIRKLQTISEETLKDELLSQLVNDAVKIYNIQKVEPNVLYSSYLQDITELLAVAIPNKTKIQIPDWLNAYRYLIQIAEFIKCGKNLYNEQIEQINGMLQSNQWDQLIYMKELPCDIQDVLQEQLVRILNPTIDIYRNQKQAIVLIDGFQQLNLENIEIQLPPEPVQETVKLWECEVCTFAENAWDSSHCSMCDTPAPANKTPYKGTEGQSQQQEQQQIQINVDEEDEKADRREKQFLEDLIEKIKEKIKTYYTDKQRDKEQKIKQINQAIEIQQNLLKQLNKQQEKKQKQLTYKSKLQQNKQPQQQLQQQKIQDCKLKLQQLTSELQLIQEIKLENNFQIYTGKQIFEKAKAFLQEFLSNRLEYFRKQINPKQLEDISSMDPIESWKYLESLGYDLHYQQINNPKIIQILPIQQMKNLLKLIEFDICKETKAVRTYNPRNIRNQIDEQAINYSDFINCLTKYQDLVNAPLLVVRQSWAIIKLFNRYLETCGQFINMDVPIGTSQQFHQNTIFLSMGNYMQVLRQLCMTPTKVDIVQRVLNKTSISRDSPPKINVERIRLANHNGKASQQDYLFTMAYEQMKNIPPTLLRPIKPQGTDPFLAFEVNFKGEDVQGESGPYRQFFADISKELQSSQLLLYPSTNNQAKLGNHKDKFVLNPSAKSNYYLHLYEYLGVLMAIAIRTATHFSLDLPSVFWKQIVGEKITFEDIEQIDATMCNLINFMQECPESTFEENIFENWVTLRTDKSVQELKENGSKIPVKYEERLEYINKLIEVKCTESELQCENLIKGLIKIIPSPLLNWVSAEDLELWVCGRPIVDVDLLKRHTRYSGDLNENSDRIKFFWEALYELSEQEKLRFIKFCWGQERLPANDEEFDRNQIRFMIKPSTVNTKQPNKALPKADTCFFNLELPNYSSKDILKKQLLTAINFDCDSMNADPRVNLDPNARHRRNREDDEDDSLFEERDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U1M5</accession>
    <name>A0A8S1U1M5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HORMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0310044</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8157872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U1M5"/>
    <dbReference type="OrthoDB" id="297310at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8157872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="184" mass="21404" checksum="152FE1EEB3B81F54" modified="2022-10-12" version="1">MNQQYINLITNLFSLSLTQLLYKFQVYPAECFHQKSAVGLTLWSLKQSILSEYVDKLIQQLQDLLSILEGIEIQIKQDNKILTTYTINIVFNQENNEQFSQFDSDIQLKTLIYTLNQKLKTYKVDDKPKTFSVNFLSNGAETQNKGVQENLLQNWILIKETKKRETQLVNSLHMPNLDILVGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U7D0</accession>
    <name>A0A8S1U7D0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0320024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8158556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U7D0"/>
    <dbReference type="OrthoDB" id="294430at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="46"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="153"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="247"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8158556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="512" mass="61437" checksum="7EAF58F7E1007FEB" modified="2022-10-12" version="1">MQSQHAQSISTGYLLQKNYINKLYTLSDSVCQQRKIVIPKQISLDKEQLYEELIREKQHNKALQNEINKLKARLQCYEKDLVIQGEVDYKRELKQALIRLKNREEEVIELMQSTNYKKVIELEKTIQQLRLELGKYHIYQKFSGNTESIINDNIELFQKLQQSTQQIKELEKIKVDHFSISRKHQQLQLVLQAKDQQLQKFRERDHLSEMNVKHSNKNEFQLREELITKDEEISHLKTQLTIARKTNVEDKALIEQTQQELQQKINQHEYERQNYKERIEHLQKEIQQLSEKARNFDRYLTVNQKRKTTYVDDQQSNSQYTQIQDNDQMVKSVQKNQVEDIVTELKLTLRKNNISLQDAEKILFKGNEISIQELEQLLGREPFKVLSSNMLARYMIEDIQDKEFEYNPELKSSVPHVRSVFRTLLQHYSLNFDDQTLQTVAKACLQIFEFCTRKNISKLNQQEFLDSISQLDLQWNSKCFDCFQQLYYNKYKGFLQFDIQNIKKLFEQYQIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UC72</accession>
    <name>A0A8S1UC72_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0380041</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UC72"/>
    <dbReference type="OrthoDB" id="290798at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="345"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8162448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="533" mass="62827" checksum="60BB118FA4B3E4D5" modified="2022-10-12" version="1">MIEQNYLCAGNHQGAQIIMVSFDPALKRNQRLLCQKCLDDLQLPTKVEGFQKTLDSIQDMYLNRVNQQQFLIDKMITFIEPLQIEFQSIRDNLLKSLDALIKVVTDWKMQYQQISKTKIVYNFLDELESLVYRQQHYKQQEILERINKMNRTYTKTIINQLLILKEDPFKEIELKLQNCEKMISNLFQNYDNQTRTNTNNNISIKLKKISKIESEKGISHIIFDQTGQMMISTNQGKVGVWNFNQGNLQFQTSFIAHNSEIKCAILISNLNYYIITAADKTIKIFQRNNEIYWKCIYEFQKLKNSIGCMIINGNQNNLFTSSENIISVWRLNFQQNQMLTCQQLLEQHKWAINAMSLNKSENKLVSCSKDKKIIVWGLNKNNQWEYKHDVRQSILECGNALYFLKDSQFIWVSGEMYGLDCIYVYEEQSEQFQEVLNKTIKLPKNQNEVSISPIVYIDQQKKIILISQKNSIYLLREKSDGNLCIETQILRAIQSHQQSQSLTQDGKYLVYSYYNNKDDRNYFSVNEIIQTSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UEE8</accession>
    <name>A0A8S1UEE8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0350291</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8161026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UEE8"/>
    <dbReference type="OrthoDB" id="287672at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000015">
        <property type="entry name" value="Oxysterol binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF148">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="921"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="921"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="945"/>
            <end position="954"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8161026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="954" mass="112068" checksum="6E19CE6CF8CC03B3" modified="2022-10-12" version="1">MQQHFFYVLNEEPKDQLIIGLTNILKEKGVIKDFGLDLCLLEFDIYELQRIVLKSLFHRIYLQYFISINKEYVRNHLIFTLYIINLILRMLDNNKNALMSCSAFKESGVLKGWGERLLIYDLKQQILRYIDVKGQTQKQLLIQNYIIHPVAFHQGKWAIKFKTPQEIEPKLARFGWNNQSEALQWYDFFNGKYLAMQITKEKKEKKLQEWDQFMPESERVINQGVPSMAIKPSQVSRMRQIIPLLPQFLQNDIEIILENTLENLQGFSDIHSFKVLFQSQTKQLYQDPNDQLHMRFFLKSEMPPARIFDELSIHTFGDNWNPQVSIYQVFPTKKDCAIIYTEFDMGEKFQNNQLKQNKPQSQKDIEHEFFSQESFSKQPKSHTLNQNEKIRLIYTQKHFQIDENAYCIMKKYIGNEIHHNQIDKDKAEGTDEFKITRSCVLIQAKQEDKFKAQIIEDIYLECENREKGEEVMKRFLLNLENIDNQILQADNYIKQQTEHVLIKNADDDIDNLQISFIQLVPEVQRITSEQHQQQSLQEYKEILDLVQTGQFIQRSIQRQFNQNILPGLEKFLETTKEDGHFLLTKYYEVDPKKGGLIYTNKKLISDQRSVLLDIIKRMGSNLLSGKSLMSVSLPIQVFEARSFLERMARGQGHAPVFLEKAAQTTDIFEQLKLTVSFHLASFMMGVQQEKPFNPIIGETFEGRIKGCPIYLEQTSHHPPISNYIMYGRGYKLFGSFCPLVNMGTNSLAGEQQGHSQIHFQNTNLKFYYMAQPFMLYGVLLGQRSVNCHKRSYCFQPEHQLLVEITFNPKDKNAGFFSSSSQKIDQFIGKICKVTPECIQKCLKAHKTNSGIKLKILPQEILDTYNINIKGRWTSFLQFDNVTYWDIEVHRPYILELESSPLPSDCLYRLDLLYMKMKDMSKGQDIKEQMEVDQRKDKQLREKLGNKNKKKKKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UKI0</accession>
    <name>A0A8S1UKI0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0410324</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UKI0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8164955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="110" mass="13487" checksum="25E1A0E92C7B1BD4" modified="2022-10-12" version="1">MGTREFDKEYKNREWEKRNAEEEKYYAEVMKQLDEEFAQMEKFKHEEDNQVDKDDQNDKLDCQKGDVVLFKNEVVQQQPPVQQEKEQIKELQEQLFKLEESEEHIKQESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UKK4</accession>
    <name>A0A8S1UKK4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0430061</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8165658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UKK4"/>
    <dbReference type="OrthoDB" id="300379at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070072">
        <property type="term" value="P:vacuolar proton-transporting V-type ATPase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021013">
        <property type="entry name" value="ATPase_Vma12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31394">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 199"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31394:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 199"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8165658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="208" mass="24680" checksum="04819DE3F59D552B" modified="2022-10-12" version="1">MVYVYIDQLPQEIKKHFKYTQDPLNPQNYAKFSEIKLYIDQYNDDHIDQLKIQEIIGTKFIMQSDLQALQIAWEADHRPKEILDEIRAREMRCKDRAFIQKSSQLTKGVFQEKKQESIKEYEKSVNFGLSFIFSIFASGLLGYYLGIYFFQLTYDQSLALAAFFLIGALIVETGLYIVKIMKEDRIRKINQRQNNKQNKTFPTKFKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UL22</accession>
    <name>A0A8S1UL22_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0430177</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8165888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UL22"/>
    <dbReference type="OrthoDB" id="307156at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8165888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="162" mass="19744" checksum="FA1C208BFE379187" modified="2022-10-12" version="1">MRSIEDDNQNAFEEYKLYEYQTITQYWISTIIFISSLTIFIGGVLLSYLFEPQLQDSIQCINLKDQLYYYSIILIFISICEIIIFLFGFGMQYADQINFFGQIQKYQRIEQIVILFTFVIIEAYIYQNRRICKINKTYYSLYAFYFWQLFWCAFVYNFENLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ULI0</accession>
    <name>A0A8S1ULI0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein eL24-related N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0410104</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ULI0"/>
    <dbReference type="OrthoDB" id="308293at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00472">
        <property type="entry name" value="Ribosomal_L24e_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.170.20:FF:000003">
        <property type="entry name" value="60S ribosomal protein L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.20">
        <property type="entry name" value="Ribosomal protein L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038630">
        <property type="entry name" value="L24e/L24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056366">
        <property type="entry name" value="Ribosomal_eL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000988">
        <property type="entry name" value="Ribosomal_eL24-rel_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01246">
        <property type="entry name" value="Ribosomal_L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL24-related N-terminal" evidence="5">
        <location>
            <begin position="4"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="95"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="95"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="144"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8164579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="159" mass="18563" checksum="98AC57E3AB8571E7" modified="2022-10-12" version="1">MVIKTDLCSFSEWRIYPGHGRRFVAKDGRLFYYLNQKSRAFSARKIKSQEIQWTVAWRRLNKKIKTDEGAKKRRIRNLKVQRAIVGISLEEIRRRRKEDDKTRKAQAEQAAREIKDRKQKQIEASKKKPAAAQKVAQKAEVKAQQKAQKGAAKTNKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UMY0</accession>
    <name>A0A8S1UMY0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0400068</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8163796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UMY0"/>
    <dbReference type="OrthoDB" id="313045at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8163796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="551" mass="65151" checksum="C74A5B73A09A3BC2" modified="2022-10-12" version="1">MTNIIEESMIINHLTSPQKQNNPNQSPLHLTPILPNSYNHQSRQSSKANLLDLQNNKTVDQQDSENVDNPMSSLIDLTQDDAKENEPELDVKQVLTEINDPQTISFQELDEIQTGNQVQLQPKLIVMNTANTIHHQIEKHKQVILQIGNKNQNQHQQQTIQIIEQQQIQQDVNKPKPIDKKMIENLKEIFLFYSKQAITTNKYNTFDKLQQLSSIMILQKFMFFCKDFELIDLEITNDLILQLGGKLDIIINKNKNQFKFNHKQNFILTKFNLVEIYKKNANSQMELTLDNFQMIIIKLSQLIFPEQNDLFYEYLGLNNPKQYRKKMLIIGKPFNSKEQTEILTQEKLYERKIYLPPKPKIRFESVKKERQTIEIQFPKVNLTARCKMKQKQNETFDKNENCINWDDLDYLQQHFDPKQFILEQDLSDKEDDYYLKEYAKHGQMQKKKIQDQMINLKELQIKIPQISIESNILSSNNNKINNNMFTTGNTRTSNNKSFDLYDNQQKKKSVGQSPQNIELTCLEKQNQIYRAFLNQQSYRERMVNKKKLILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1URE4</accession>
    <name>A0A8S1URE4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">H-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0420010</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1URE4"/>
    <dbReference type="OrthoDB" id="299596at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019019">
        <property type="entry name" value="H-type_lectin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09458">
        <property type="entry name" value="H_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035717956" description="H-type lectin domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="H-type lectin" evidence="2">
        <location>
            <begin position="41"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8165036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="411" mass="47191" checksum="6B8B80A3A54648F1" modified="2022-10-12" version="1">MINNSFLLFAQLIVLISGILKYDSGAFSGFSTCQSGNTQTYTINFNGVFGNVPQLILGLEKIDMGKGADFRIQAQNIQTTNFELQLNCVTSTPFYSAIFNWYAIDDQRIQVINNFNMENPDNQVFDHDNANAIFGILSITSMGIYHDVDFQISISSITTTQVTVSITKHNWWFVNLKQIGYQIILGIQEALTDVEILFHTSQYNSGLLTQYPNKWLFLSYNGFALNNNIRTRSVRTSVSESYIVETWYGTYINNYHLKSWIAYQFTTVFQAFECLTLRISQALDMEVQIKPKIFLEINEITQSFSSSTYLIIDKTLNLLNMKIYMKCTKTKTIASQFLKCQSCSTKKYYKFSHYCHGTIDAVTYFPRFQLAQSVYKELNVNILVDRIIITQVLYNQVQSSETLLDIQVLNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UUB3</accession>
    <name>A0A8S1UUB3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0480022</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000048">
        <property type="protein sequence ID" value="CAD8167837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UUB3"/>
    <dbReference type="OrthoDB" id="77931at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8167837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="287" mass="34183" checksum="BD132993E7FD265A" modified="2022-10-12" version="1">MEDKDQEFGLQFNSYIIKYKIINNTASYRGGGFYFEEDSQRFTIQSTLIVNNSAEEAGGIYLGGISSLNQNNLIQSLVLFNNVKLGSNNIKEIPQHLSLSINQMEMQFQKEKLITVLFKTYNQNHFKFYLKVNGQQIQNFELYDPNRLKFLSYINEFTIKFKNSMNEHVINFSTLLLIQIIQFLILQLQRQLNLKQYQHQALIMKLIVSTLDLQHLIWIRLSRKIQFMKFQFIVKQNIRQYVNKKRNKLFHLSYKHSSFQTAQSQVIHLLMIFQQFIVKNTCCKYYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UUL4</accession>
    <name>A0A8S1UUL4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">VPS10 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0470193</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000047">
        <property type="protein sequence ID" value="CAD8167582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UUL4"/>
    <dbReference type="OrthoDB" id="443634at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006892">
        <property type="term" value="P:post-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.80:FF:000007">
        <property type="entry name" value="Sortilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002201">
        <property type="entry name" value="Sortilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.270:FF:000002">
        <property type="entry name" value="Sortilin-related receptor isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031777">
        <property type="entry name" value="Sortilin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031778">
        <property type="entry name" value="Sortilin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006581">
        <property type="entry name" value="VPS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050310">
        <property type="entry name" value="VPS10-sortilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12106">
        <property type="entry name" value="SORTILIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12106:SF27">
        <property type="entry name" value="SORTILIN-RELATED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15902">
        <property type="entry name" value="Sortilin-Vps10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15901">
        <property type="entry name" value="Sortilin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00602">
        <property type="entry name" value="VPS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="745"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="domain" description="VPS10" evidence="4">
        <location>
            <begin position="57"/>
            <end position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8167582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="804" mass="92612" checksum="6E9C5D6BC2886095" modified="2022-10-12" version="1">MIIILGLLILFVQGQNEVKVSFNELESPVSNIYWCGSSVVFTKDDETIEQTHQESRKVLFILTDKGRIWRSADYGSTWLDETKSWEKMEPENKQLQFESIHISPADSRVIFFFGSNGISYKSKNCGRTYTRFSHSEDLYDFKLNKMDPQWIMAFKDKPCGKNDNNCKDFYKKSIYVTEDGGETWKSALNYVRDAAWDKLLQYQLIPDQRIIVCHMKEGKSVISYSDDYFTTIQTMQENALGFFQTSHYIFVLTTGEDGETGYELLIAPAYLDKFQPQPVQLPIPLNQHTFTILDTSEGQIFLSVSHKEENQRLTNVYVSDFRGFKFTLSLLHNVRSLDTGNCDFERMLGMEGVYVANVFDHQEVEKSKSRSSITPATLELYKKTFISYDRGGQWHPLKAPEIDSKGDEIQCSGDCSLHLKGRTEANQNPLYSSQNAPGVSIGVGNTGLYLTQKDHEVNTYLTRDGGHQWFEIRKGSHMYEIGDRGGLIVMGQDDKPINKVIYSWDQGFSWEEVKISEKEFEILNIVTEPSNMEQKFILYGQSRSQENQLKGYVVALNFQTLHQRVCSGAWDPTMPESDYEFWIPKNFESGKCLFGRKIKYIRRKREAKCFNQEEIDKKFFIETCPCIEDDWECDFGFYRKIEGGPCVPIADKFEEDDTPDLLKPPVNCKQTYMKTQGYRKVSGDYCQGGIDLSPVETPCPNTQTNNTSFNNTEIPNIDIQQPQISKPQIKIPQTQYNNSQKQYSYLWYVVAFLGVIIVILFLKDKIMKIFSSKPPKKSSSYMEKGNYEQLKLFSGNDEDDEAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UYG9</accession>
    <name>A0A8S1UYG9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0510121</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000051">
        <property type="protein sequence ID" value="CAD8169544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UYG9"/>
    <dbReference type="OrthoDB" id="296677at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06463">
        <property type="entry name" value="p23_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CS" evidence="2">
        <location>
            <begin position="116"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8169544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="305" mass="36416" checksum="041F08DE6A8FE396" modified="2022-10-12" version="1">MLFLTFLISVSMASQDLLKKCYLEQFTIGDPEVKIQIYFEDHIVKNHKIEYECLEFIISRGYYKVALSLYENYFLLNHIDITDRIVQFLKNDKYLNQREMQTLFKLAMAKSNQVQIVQPVVQWAQSKNATFINIKFSHRQDAPACLNAKLEVVEIKNDSLLIEAFGIVSHIPFKYRYAIKLYKPIDPTSSYEKVESVGTMYVNLTKIEPVLWLRLTEEDYKTPIWWDLKDNFRKDMEEFAQMLEKESERKERNADKQAKKNQKKRDQEKQKQTSQKAQEAKRQLEYEHNQCYKPGKCEIGWYQRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V1H5</accession>
    <name>A0A8S1V1H5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">uracil phosphoribosyltransferase</fullName>
            <ecNumber evidence="4">2.4.2.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0540194</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uracil: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPRTase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.9" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8171238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V1H5"/>
    <dbReference type="OrthoDB" id="106623at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004845">
        <property type="term" value="F:uracil phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008655">
        <property type="term" value="P:pyrimidine-containing compound salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000023">
        <property type="entry name" value="Probable uracil phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001097">
        <property type="entry name" value="PRK00129.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14681">
        <property type="entry name" value="UPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035790709" description="uracil phosphoribosyltransferase" evidence="10">
        <location>
            <begin position="25"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="11">
        <location>
            <begin position="56"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8171238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="266" mass="30001" checksum="86E5E4DF8E73D3EE" modified="2022-10-12" version="1">MNSRLLKIFKTGFLGLIGFTLIAQQQKRFQCAAPYFSFISHSELQQFRLRFPSLYVLDKFQVEHLLSVIRNQTTQTKDFRLYSDRLIRLLMEKAISEHSKKLTPPEGSQNQTPAQEIQFENKQFCVVVMVRSGNAFLSEALKVLPGASVGFILVQEHPQTKDPQLIYCKFPEDIDQKQVILTDAMITTGGRISTAIKALQSNGVNQENIAAVNIVSCEKGLSKVLHQFPKVKVITAGVDYALNTIQDHRFPGVGDFGDRYFGTVDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V1W7</accession>
    <name>A0A8S1V1W7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase S11 D-alanyl-D-alanine carboxypeptidase A N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0540027</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8170931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V1W7"/>
    <dbReference type="OrthoDB" id="10254188at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009002">
        <property type="term" value="F:serine-type D-Ala-D-Ala carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.710.10:FF:000096">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001967">
        <property type="entry name" value="Peptidase_S11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21581">
        <property type="entry name" value="D-ALANYL-D-ALANINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21581:SF6">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00768">
        <property type="entry name" value="Peptidase_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S11 D-alanyl-D-alanine carboxypeptidase A N-terminal" evidence="2">
        <location>
            <begin position="66"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8170931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="331" mass="37590" checksum="56334C7261DA6E97" modified="2022-10-12" version="1">MFKPPLPNQRLSQKALDQPLILIRSIGSSQGNRPNRTSSDSRHRPGSSQRSISFSKTDLHQKYGVPLVTAQNFIVMNGLDGQNLTSFNSKKRVQVASLTKLMTCYVAIQLIRDLKINIKQIMYVSYDAVQISGTSAFLRPYDKITLYDLLHGLMLPSGNDAANVIAENLSSFIKDPVSNIALQYRIQPFIQLMNQYALLLEMNDTQFYNPHGLSNDQSYSCAEDLAILCKQIINDPMLMEIVNKKEHQTTLQRNNKLINLTWKNTNRMLEFEGYCGFKTGFTDKAGPCLATLYKKDSICLIIIILNCLSKEVRWDDTIKLSNWGQRKLNVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V1Z9</accession>
    <name>A0A8S1V1Z9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Alpha-type protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0510266</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000051">
        <property type="protein sequence ID" value="CAD8169819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V1Z9"/>
    <dbReference type="OrthoDB" id="308155at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04515">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00198">
        <property type="entry name" value="vWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004166">
        <property type="entry name" value="a-kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056861">
        <property type="entry name" value="HMCN1-like_VWA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052969">
        <property type="entry name" value="Thr-specific_kinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47763">
        <property type="entry name" value="ALPHA-PROTEIN KINASE VWKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47763:SF5">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_25, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02816">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25106">
        <property type="entry name" value="VWA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00811">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51158">
        <property type="entry name" value="ALPHA_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="VWFA" evidence="7">
        <location>
            <begin position="242"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-type protein kinase" evidence="8">
        <location>
            <begin position="483"/>
            <end position="719"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8169819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="914" mass="106600" checksum="F415573B36B8C431" modified="2022-10-12" version="1">MISKLIEQSSSICPENIQCKNKECNLMHPRCFAGVCINNLNGKCKKPCPQNLNHIGKGNLKEKLQMISINGVFVINLCPKVDCKNNNCYYLHRDWIKKRNICMKNLKNSDDCEGECENNQKHFDWEELRQEVLKEYEYKNISPEAITDVEKIYDSLEKYCLKYFKGICDYKGQCPKFHVDWENIRNVSADQFERKKLIPCTHSLKPQLQRMDSKSYKKLELRFDKQKLQKLQDDIKERNILDVVFIMDCTGSMEIWIDSARESISSIIDIFNKTVSQTLIRIAFVGYRDFKDDKHIQYHDFTTDVDLIKQFISTLEATGGNDQAEDVAGGIEQVLKLNFSKHPDSILCTFLISDAPTHGKQYHDNKVEDEHNDEIKDGSLEKLMQVLDQKKVPNNYFFCCKINDTTNKMFEIMKQNFKNLEISQLETQDFANYVSISMIKSYKKSSIFFMNDIQSKTQRFQAHFIWHKAIEPVECNSKNELNYFSNFLTQMGKNQVGGDTILEIEMNQKVLSQNDQGMVSYVFEAFDIRNNLKVIIKLPKKVVDQYQCRKGQIDEDLIQNAQKASFVRYNQTIIAKQLSCAYRLQTKSNDKAPPIFYVTPIKYTLCQEFMGLKDLYAETNININGPWEKISNNTNYIDKEKENYSAFSHFTYHFTNKSLVITDLQGKQGVLSDPCIHTKQVIGYLRDETNQGILGIETFFQTQHSQCNDICKSLGLKRNCDDFNIPIPGKRAFLEIKQLNLQNVNAVCNVCGDIFQIIYEDYKSQIDTAYTCKSCLEINEFTEICECCGNEFKFNLNRVLKQALDLKICDQCQKDCLDQRCNYCAQILQKQITYCEMCDKKQQCYYCKSKCSRRNVKQKVNATNGVSIYLCEEACQFFQNLYCFKCFSEFSYQSWPSQTEYIKGEYICYKCQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V222</accession>
    <name>A0A8S1V222_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0550014</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V222"/>
    <dbReference type="OrthoDB" id="304281at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035735131" evidence="2">
        <location>
            <begin position="17"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="114"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8171418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="392" mass="44905" checksum="CC6C1B3357EB5BC3" modified="2022-10-12" version="1">MKISILLSLLVLGCLTMDFDQQIEELQNSKFGQTILQTIMMELQTEDPVVSNLINMVQGIETTLENEQQRDDDRIVRIRQNCDIDITTLNNQINQNTIASLGLKSQLDSLNPQKVQAVASLERKNNEITDLKAELQYQSHKRETETAAYETILDNLEQALFGVNQVKGYFNSYLDILVKNRKRFEKPSFLEESYSFKYEESVQDDSESRSLTSLAQVAQKVNKIKHHVQLEGYASMLEIMSELASKAADEPSQAEVLTRKVLSVLKQIENYIQSERIREDQAEALRQSNFDLLNTLLSDQLVQANQDKTYMEGLVASLSTKITQGTNEKFEVDQKVAIKTKELENRKTDCRLKNNEYETDTQNRIKQKRVVVVAVDLISSKLGQLKRKLLEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V5G9</accession>
    <name>A0A8S1V5G9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CBM20 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0550133</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V5G9"/>
    <dbReference type="OrthoDB" id="550577at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001070">
        <property type="term" value="F:starch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05467">
        <property type="entry name" value="CBM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002044">
        <property type="entry name" value="CBM20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15048">
        <property type="entry name" value="STARCH-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15048:SF0">
        <property type="entry name" value="STARCH-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00686">
        <property type="entry name" value="CBM_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01065">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51166">
        <property type="entry name" value="CBM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM20" evidence="1">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8171653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="184" mass="21407" checksum="CDFB752BA7E4A460" modified="2022-10-12" version="1">MNTQVFFRIYCKTQFSQHVKVVGNEPQLGNWNPSKGLQLLTNESMYPIWFTDYPLELLAYSKLEFKVVIIDDQNCYWECCPNREMNVQSLKQIVIVNYDNPSIQVLGIKSLVSEVELPNIEQNRARKVSIQVQDYMSYTDSDSEDQNSKKSQLTNDNSLFPSIESFNSCLLPNEDLQQDRIDMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V9Z5</accession>
    <name>A0A8S1V9Z5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0550188</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V9Z5"/>
    <dbReference type="OrthoDB" id="1700726at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF33">
        <property type="entry name" value="AMP-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="3">
        <location>
            <begin position="64"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8171746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="668" mass="74988" checksum="70B09AABC3A5134A" modified="2022-10-12" version="1">MGCGVSNAHQFMRYSQEKITENEPGSLEQNQGQTTIRRNVKYANQLLMIPQQGITTLQEMINNSVAKFGNQPCMGKYEGDNFKFMSYNQVNEEAIHLGSGISNLNLVKEVQEYQHYKLKLIGVFAKNRREWMILDWANIIYGYTMVPFYDTLGPESIPFILDQTNIETMLISGDATKSLIQCKEKYKLKNLVLLDPISDQQTSELKNKGYQLFKYEEIIENGKKQIVQPAQVLPSTIFTLCYTSGTTGNPKGAILSHGNFISSIASTQATDAGINQNDVHLSYLPLPHVMERLIILTMLFTGACIGFYRGDPNLLKEDIQKLRPTVFASVPRLYNKFYDGIKAKINEVTGLKKSFAERAIRVKLENLRTEAKYTSGLYDKAFQGVRDLFGGRCRLMITGSAPIQQEVIDFLKIAACCPILEGYGQTESTALSFSTGVWDPKSAHLGGPAANTEFKLVDVPDMNYTSLDVVNGVKTPRGEICLRGYGVFQGYYKDPEKTAEAIDAAGWLHTGDIGLITENGGVKIIDRKKNIFKLQQGEYIAPEKIEAIYNRVQGVAESFIYGDSLQSQIVAIIVPQKEFVEKHAAEKQIQGDFEQLCKNPEIIGLFQKNINDYGRANKLNSLEIAKLIHLEPQPLQNFGCLTSTFKLQRHIAKQVFSKQIEQLYKSQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VA15</accession>
    <name>A0A8S1VA15_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0580087</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000058">
        <property type="protein sequence ID" value="CAD8172849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VA15"/>
    <dbReference type="OrthoDB" id="298777at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8172849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="572" mass="67641" checksum="704668C0EE144721" modified="2022-10-12" version="1">MQGKYSQSNWYFQPTDFFIETTLDGKLMYIKDGEVLKQEKFNNIQSGDILRNLEQIKNLTWYGQYSFKNKKIGNWQAYWQGKQLKVGGNYDNKGKKIGKWIELFDNYWDLCKLISEGEYQNGIKIGIWQYYYDGKMISLGIYNNEGLKNGNWVDLYENFWSGCQVKFAGTYKSGLKQGNWDTLFQQKYYDTFNKMYFNNNFSGGGFYDKNGIKQGNWIELQESFSDSFLVTSKGEYRCGKKVGRWNIYFGCSEIQDYHIGGGLYDDNGGKIGVWIQLCDNQSRYLNLKKIENQIQFRRVNIINKKKLEDGTIYLKIRQSEEEFTIKMEKNQINGQNCIKIFQSNLFKLDLQQSYCQIIYIGQYFEGIKQGRWDIKFKYKEVNLKDYGKIGGGMYDKNGRQNGMWIDVDETFSNQSQVSYQGEYNEGIKIGKWDYVLNRNNKNKLDIFGGGFYDQDGKKKGKWINFHTKFSKYLNKNFIKISDFQVIYLGEYKNGIKFGKWENKYRFETKEQLKNSGVGNYDHIGRKIGKWIYIDFEFQFGYELIHIGNYFKGIQQGKFLKKMLPLQSLIQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VAD7</accession>
    <name>A0A8S1VAD7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CDC20/Fizzy WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0600067</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000060">
        <property type="protein sequence ID" value="CAD8173807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VAD7"/>
    <dbReference type="OrthoDB" id="284945at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010997">
        <property type="term" value="F:anaphase-promoting complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990757">
        <property type="term" value="F:ubiquitin ligase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905786">
        <property type="term" value="P:positive regulation of anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033010">
        <property type="entry name" value="Cdc20/Fizzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056150">
        <property type="entry name" value="WD40_CDC20-Fz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918">
        <property type="entry name" value="CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918:SF1">
        <property type="entry name" value="FIZZY-RELATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24807">
        <property type="entry name" value="WD40_CDC20-Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CDC20/Fizzy WD40" evidence="6">
        <location>
            <begin position="160"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="217"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="293"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="432"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="31"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8173807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="484" mass="55189" checksum="DFC7F3645D50272C" modified="2022-10-12" version="1">MQQEQIPQQQQNVTPKKYQLTTPTKDKKQNKTNQSTTIQSPKNNDFYSPKNVNPWKTPKSLLKSNHKQNTPDHCIQASPYSDRYIPLNVSRNLFNKQIQPFEIEEENQYEELLSENVLEIDENKHVSILNFNKQKHDKVQSSKQLETPKRKIDTLPIKVLDAPGLEDDFYQDTLHWGKNNLIAVGLQRCVYLYNVDNSKVFQLAEPINNNELSAYYTSLQWNTNGQILAIGCCDGSLKLWDYNKNSFNGSMNLSNKRISTISWANPNIFAYGSKDKTINICDVRVPNYQIFQLLGHTQEVCGVTFDGNELQLASGGNDNKVFVWQMRGGNSNNNNNNQYISWEIKSHKAAIRALAWNPNSCGILATGGGNQDKTIKIHSSLTNQQITSINCDSQVCKLRFSKIVNELVSTHGYEKNLVCLWQYPTMKRVNQLEGHSERVLYLSASPDESTILTGSGDETLKFWKIFPSQVSNNISSLFSMCEIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VAV2</accession>
    <name>A0A8S1VAV2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">40S ribosomal protein S30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0600013</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000060">
        <property type="protein sequence ID" value="CAD8173701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VAV2"/>
    <dbReference type="OrthoDB" id="302928at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006846">
        <property type="entry name" value="Ribosomal_eS30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12650">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S30/UBIQUITIN-LIKE PROTEIN FUBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12650:SF15">
        <property type="entry name" value="RIBOSOMAL PROTEIN S30, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04758">
        <property type="entry name" value="Ribosomal_S30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="25"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="47"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="67"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8173701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="92" mass="10815" checksum="C51E1D00AFC3E24A" modified="2022-10-12" version="1">MGKLHGTLAKAGKVRKQTPKIEKQVRRHKIPKGRAYKRICFNRRFGSATTSAQGPQQRKKGPNWHAGRKDLIEEERKKQVEQRRQRKKQDTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VDZ0</accession>
    <name>A0A8S1VDZ0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0610282</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VDZ0"/>
    <dbReference type="OrthoDB" id="310386at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="98"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="132"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="39"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8174781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="258" mass="29927" checksum="445572A5A425C22E" modified="2022-10-12" version="1">MEQDNTILDQMKQQLQKKDYLTFKSQINIIKRYYSKEKENQIQKQIIQLNNILETLKNMVIELTKNGDYIINGDQNQINIQQIIVESEDKKQQNIKEAERLLNEGVALNNLNKYQDAIECYDKAISINPKYDKAWSNKGDALNNLNKYQDAIECLDKAISINPKFDSAWNNKGSALNNLNKYQDAIECFALHKLQKYTDAISCYDQALSININPLRLQRKADSLFELGKKSEAKQFYLASLEKGSNDKIYIQKQLSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VEZ7</accession>
    <name>A0A8S1VEZ7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">14-3-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0620109</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8175013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VEZ7"/>
    <dbReference type="OrthoDB" id="303294at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000308">
        <property type="entry name" value="14-3-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023410">
        <property type="entry name" value="14-3-3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18860">
        <property type="entry name" value="14-3-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00244">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00305">
        <property type="entry name" value="1433ZETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00101">
        <property type="entry name" value="14_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="14-3-3" evidence="1">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8175013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="122" mass="14080" checksum="EB7242580F8DCE49" modified="2022-10-12" version="1">MKSDTIRYLSEITTNDKKQFMFNKQSNIQQKIQDIFEGGISKIDPKWLGLALSQAVYTYEILNDKVSACKIAQKAFDGALSELDQISQDNYKDVTIIIQLLRDNLVLWESEQMNEANNKQKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VJG3</accession>
    <name>A0A8S1VJG3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Alpha-type protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0670041</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000067">
        <property type="protein sequence ID" value="CAD8177137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VJG3"/>
    <dbReference type="OrthoDB" id="311121at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004166">
        <property type="entry name" value="a-kinase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02816">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Alpha-type protein kinase" evidence="5">
        <location>
            <begin position="610"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="74"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="174"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8177137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="791" mass="93857" checksum="93186A8B6630807A" modified="2022-10-12" version="1">MSNCKIICEMPNKEIIHIDCELQNVKNELLKKTNQIHHENMILIDISETQYKSSNSETQNNQMKDLIYFKVYDANQLYKEFKEIENIKNEQEKQIIVFQDKNTKLELELNQIINKSNSQKQKIKKLKEENQKQQNNNHELHIQVVSITSEKNAINQVWQICLNQMSQLMQQLYLNAAQTNNTFQIDNLENLQIQIKDLQDQLQIQKQDTDKQKKLLKQEKDKSYDSQTQSENTITQLNEKIRKNERDIKHLIEKNNELEMNYRNTHMEVQGANQENKKLLSQIKQLTSTIQQLEYDKQELQNHIQSLEAKIVSQIQQIFNLNQNIDQLNEQIEQQKEEIRKKGLESDKYKNEYKHLQLQKESINKECQELKKQQQRLQEEIEIKENGGQVNQNEISRLKNQIIKLEKDINDQNKLNQENQEKIRQFKTQITTKDGEISNLEIKLNQLQTSLKQKDEQIRNDQAANQLFKQQMDVIIQGMKSEIEQSNQKLEIKDHILKKLSKQMQDDKYYDKVIGLMDKLMQSPNKIYQIEKDQTAQFDLKDYEIFEITFEFNKAIELLKNNQYFLFEQCLNKNRKGNVKLNRSIQPNAKSTFFKGYLSSVKEADQNEFKGKYFLRKDLISQNDPFTNIDEAIEMSKNNFLCQLLMEQFNKTLKSQKLKEGDYQIARQFILKSKDKEEYYYCEMVEEGEFKKINGGAFNPKLSETDSYFNAFSKFVYAFSKENYIITHLQICGKKVHDMIVSTTYKGLFSTLDQGSTEIGQFLEIINNQPANMINQHWRQIVEDIAKEGFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VK31</accession>
    <name>A0A8S1VK31_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0660091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000066">
        <property type="protein sequence ID" value="CAD8176963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="321643at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002895">
        <property type="entry name" value="Paramecium_SA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01508">
        <property type="entry name" value="Paramecium_SA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00639">
        <property type="entry name" value="PSA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035886345" evidence="1">
        <location>
            <begin position="20"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8176963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="366" mass="40544" checksum="5655E7E3A2D61942" modified="2022-10-12" version="1">MYKLFILFSLLLAITTSQKLSITQCTCAQLLSEGDCIKNASIRCSWNSTTKTCAVSTIPTLTYATYCDRFSEANCPKIKPCTDCGSYAACAWVEGQCTHFTGCTAFAKKTEPECQAISNRCITDGTHCVELDACSTYKKQLPCVKNAAGRLCYWDTTNNTCVDANACDRLPITFVTDKECRDVIPTCTTKTGGGCVDSGNNCSDQTLEIQCVWNKLGTMACYWDGAACKDRICDNAPTTLTTDETCKTFRTDGTCTTKPNGGCITRTTCAAATIQAACVKNSSGGDCXIHYFDFYQLSNMLYYNIIKKILKTFLLPSMLLEIFKRLSLIQQIQTQFVNTILKFSLIIDFINKYSFQQNEILKMPKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VK41</accession>
    <name>A0A8S1VK41_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0640178</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8176119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VK41"/>
    <dbReference type="OrthoDB" id="311989at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8176119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="195" mass="22786" checksum="89CFC36204C54968" modified="2022-10-12" version="1">MELGGKDLFSVLKNKLQIEQKIKICQEISQSIAFLHKQQLIHRDIKPENFIQVGTTFKLIDFGLIKKKEENVRLTKMIGSPLYQAPEIIKGSDNYQASVDIWSLGCLFFELFQGEPLFDGQTLQEVYQQILNYCKNQQQIHLKINQLQIKQELKTLIKQMIDPIPDKRPNIDYVQKELLPKFLPYPNKNILKQSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VRE7</accession>
    <name>A0A8S1VRE7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">10 kDa chaperonin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0720087</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GroES chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000072">
        <property type="protein sequence ID" value="CAD8179527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VRE7"/>
    <dbReference type="OrthoDB" id="184876at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00320">
        <property type="entry name" value="cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.33.40:FF:000002">
        <property type="entry name" value="10 kDa chaperonin, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020818">
        <property type="entry name" value="Chaperonin_GroES"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018369">
        <property type="entry name" value="Chaprnonin_Cpn10_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772">
        <property type="entry name" value="10 KDA HEAT SHOCK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772:SF0">
        <property type="entry name" value="10 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00166">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00883">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00681">
        <property type="entry name" value="CHAPERONINS_CPN10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8179527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="100" mass="10937" checksum="23A3DC44080F12A6" modified="2022-10-12" version="1">MASQFKKLAPLLNRVLIQKYEPVTKTASGILLQTSEEKQAVGKVVEAGPGQIDSKGNVMPTLVKPGDVVLLPDYGGQKIKLADQEYFIFRDSDIIGILHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VTB3</accession>
    <name>A0A8S1VTB3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0740204</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000074">
        <property type="protein sequence ID" value="CAD8180600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VTB3"/>
    <dbReference type="OrthoDB" id="288803at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8180600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="107" mass="12173" checksum="BF56157D01C9CAC1" modified="2022-10-12" version="1">MGCNVQKSKSQSNKLCQAPQEILESARIANEQDGLTHGQKSDIKESENLVSQQQQVQIGRRLKHMASSDELNKFINKKHSNPQSHFGYIKNSPEQRRKQSLMNSPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VTF0</accession>
    <name>A0A8S1VTF0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">6-phosphofructo-2-kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0680087</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000068">
        <property type="protein sequence ID" value="CAD8177796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VTF0"/>
    <dbReference type="OrthoDB" id="267323at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003873">
        <property type="term" value="F:6-phosphofructo-2-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004331">
        <property type="term" value="F:fructose-2,6-bisphosphate 2-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006003">
        <property type="term" value="P:fructose 2,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000006">
        <property type="entry name" value="6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000644">
        <property type="entry name" value="GpmB, Fructose-2,6-bisphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003094">
        <property type="entry name" value="6Pfruct_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013079">
        <property type="entry name" value="6Phosfructo_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606:SF44">
        <property type="entry name" value="6-PHOSPHOFRUCTO 2-KINASE_FRUCTOSE 2,6-BISPHOSPHATASE LONG FORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01591">
        <property type="entry name" value="6PF2K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000709">
        <property type="entry name" value="6PFK_2-Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphofructo-2-kinase" evidence="5">
        <location>
            <begin position="6"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="3">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="304"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="225"/>
            <end position="232"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8177796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="447" mass="51970" checksum="64968528E45656AB" modified="2022-10-12" version="1">MQDCHKSQKIALCMVGLPARGKTYISRKLHRYLNWLGYNCKVFNIGNYRREICGTECDKSFFDSKNEQATKARNECAQMALNDLINYLNSDGEIAIFDGTNTTKERRNMVQCSLLQQCQNVQTLWIESICDDADVIQNNIRLTKLNNPDYVSMNPEDATKDFLGRIKMYEEVYQKIDLDENLSFIKLINIGEDIQIHKVKGYLLSKIISYLMNLHIYPRPIYLTRHGGSTFNQENRVGGDCDLSQQGIQYSEQLGKFLLDEFPTQEQRNQIHCLTSTMKRATHTAEIVCNILKINYLQLKTLDEINVGICDGLTYTQIAIQFPEDFAERKVNKLTYRYPRGESYLDLISRIEPIIYEIERSREPVLVIGHQAILRCLYAYFHRNEISEVPQLEIPIHAIIKLTPAAYYCEEIRIKIDPETGERKQIEEDLQIARINMLTKKKSYILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VW41</accession>
    <name>A0A8S1VW41_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0770187</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000077">
        <property type="protein sequence ID" value="CAD8181984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VW41"/>
    <dbReference type="OrthoDB" id="296912at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="397"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="398"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="409"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8181984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="457" mass="54610" checksum="AEF915FAA5C7A47A" modified="2022-10-12" version="1">MEKLTIEQLFKIIFEINKDQHFIFCYACQIRFPIVEPEKATLYIHHFSKDHVYNLSTIATKEIMKNKESTKNDNRISQAIPLFDLEKALNIQQQQSKNVLHQYLKDCITKLPNIYDQLELNNLEAYMSTLRRLYLHLLNHNTSFLTLELCFSFLDIYKTDKDLQLIRKIVRALNFPLSHEQMQILNPHKQTYPNLSQDLYIEYYQIFINDLIDLGLSKESLVYHLAKIGTPINKIIDLKLYEIKNKINDLINKGINEEQNIFFLTIIEKLERYENLNSHSYVFTKWNTECQIRKCVRQIRNRLRDQTTDEKYKDFLATASLQQIQNWHKFQIQNEKNNTKEMISILETINENKNQSRKKKKSIIKQFTIPNNSNLEQFERKEQEELENNEFDISIIDSKSERDMDKGLNNKQINQTTKQNSIDQKNQQQCEIINTESQKEKLENYQHKALLKKSSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VX00</accession>
    <name>A0A8S1VX00_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">14-3-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0770126</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 14-3-3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000077">
        <property type="protein sequence ID" value="CAD8181880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VX00"/>
    <dbReference type="OrthoDB" id="10260625at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08774">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.190.20:FF:000001">
        <property type="entry name" value="14-3-3 gamma 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000308">
        <property type="entry name" value="14-3-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023410">
        <property type="entry name" value="14-3-3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18860">
        <property type="entry name" value="14-3-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00244">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000868">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00305">
        <property type="entry name" value="1433ZETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00101">
        <property type="entry name" value="14_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="14-3-3" evidence="2">
        <location>
            <begin position="4"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8181880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="247" mass="28163" checksum="12198188C5E174EF" modified="2022-10-12" version="1">MTSRDELLYMARLTEQTERFEDMVNYIKQLVSVGQELSVEERNLLSVAYKNSIGGRRTAWRVLSSIENKEEGKAQTNPASQRNLTLIRAYKKKIEQELNGFCNDVLNLIDTSLIRTATNSEAKVFYFKMKGDYHRYISEYSSGDQHKHAADGALEAYQQASNVANSELKTTNPIRLGLALNFSVFYYEVLNDPTKACSLAKQAFDDAIADIEQIEESQYKDATTIMQLIRDNLTLWTSELEDEEGPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VXK7</accession>
    <name>A0A8S1VXK7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0740210</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000074">
        <property type="protein sequence ID" value="CAD8180612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VXK7"/>
    <dbReference type="OrthoDB" id="282355at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8180612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="140" mass="16445" checksum="B37449FFD1D794BC" modified="2022-10-12" version="1">MGCVKSNGNKKLEDMKQGFVIRILHPDNRTEKQYKYFDEKQKSDLIMNVMNGICFSEKVSDKCDGNFISVYDPADDRFHYYIQKLDALGIEIDNPNEPLKGRIWVPYINEKKSDWDILVENNTRISITDHLLWKLEAVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W275</accession>
    <name>A0A8S1W275_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0800050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W275"/>
    <dbReference type="OrthoDB" id="10692747at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="255"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="447"/>
            <end position="648"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8183043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1014" mass="121452" checksum="375FCA924EADDA57" modified="2022-10-12" version="1">MFNNKSKKPSLLYVECKSRDISRRNSRISDLLNDTMNKENSNYQSTFIPNTQKKIDRTVGSLNKMYKQAIQDQQILQEQASMLMEDLNGKLLQRAELIGQVEELEELIEFHKIQSNFEFTFATKIEQLDLQIQQLKKSSSFKLTGNTADLIRDREQQHKVLKEKINQETILAMTTYMQQWGHQFENIKNQYGNDVYQQVLKEQEQQINQLISQQYNKKIQGIIQIIRNHEKLEKAIQLNIEYVDNSLNEDIYIIKQTMNQKKQQVQNIRNNLLEEDQSKFQLLKRVEVLEQENQQLEQQLNEITMKKDLYQQEILNKQKTEEIYNEILTGFDCLSQIDNDNEDQKSDNSNSQLKSCTNILSNINKQTKKVLSSHLNKDDSLHFSQLQQYLQINSIDGENNSKCSSACQLSADDSLLQHFNELNLTLLQSEKSKNNQCNLPLQQQQQYYQYQQQYQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQYNYFNQKNLPIENQTQSIDFIRLQTITEEQLSSKREKSKMEQSNKINTTPIHQNYLNLNKVKIVDNSSQNITAPLSEQTKRFTNRLGQSQLQMSNLNEVTQKQISIPSPYMPLFFNNQPSNPQTQKSQQPQVFRFKEQNSLSEQIQKHKKSKSFDSTLNKDIQQRQLFCNVSTNNGQTYYNSKDSQYLSNKSNHLVPIEQEILRITKPLQKGVLIFKRFTSTKPNLTKMEFDPFTCQNPNVCGYCPRMMTLSQNLDKIEFKNQMRINQVDSLLQLDQIMRVIIPKTIQQSILIKKQIQKKFILSQEERIVCGILYWPMSIITQNDGRIELLFDNEEILEQWQTSLIFLKDNIKTLQIINKKLKKQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W2V2</accession>
    <name>A0A8S1W2V2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0790026</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000079">
        <property type="protein sequence ID" value="CAD8182612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W2V2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001751">
        <property type="entry name" value="S100/CaBP7/8-like_CS"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00303">
        <property type="entry name" value="S100_CABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="217"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="64"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8182612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="239" mass="28349" checksum="B68B152CF2815C59" modified="2022-10-12" version="1">MSPQIVDQKQYNSKTQDLMNAYKQIKARNYHGKKSISLILYPNTSLKLLKAQNLINDIRQRIYKKKIDIHKLIAHLDISKDKCLDFDEFGKFLMKVDKDITRQKIDYIRIKPNLKFGLKIIMHSNQNIYQFILNGNAQQQQRNGSYKKLSNLPHQLKSQDSIHEKNQSILEKLKLSIQKQKKIYKTSNLINVLIRDWIKIIVKKNRASYNLSRNNCWFLLLLMKMVIAILISKNFNKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W331</accession>
    <name>A0A8S1W331_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0790066</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000079">
        <property type="protein sequence ID" value="CAD8182672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="71437at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8182672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="611" mass="68978" checksum="17E871C907AE8D2B" modified="2022-10-12" version="1">MLQQQFSYELQSKPFYELQLCLAIAIHHNHKQLIAGDYTQIKLFQFKDGVLRLLKILNNSGDITTLNYFKTKPNFISGSYDAFLLLQPQTNISSSKYLVKLPKCSDSINCLVIHPSEDLIICGSNSIEFWSSYSEITQHQQWVQYQTISHENSVYLGLSLNQNGNELISCGNNNVILVMKGSNTQKWIIIKTINVENNGARXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQSFTLVKIQQFVVQILLNFGLLILKLHSTNNGHENSVYLGLSLNQNGNELISCGNNNVILVMKGSNTQKWIIIKTINVENNGARLAYILDRIFGFQPYKSNYLQIYTSNKLSGDYIKSQNIQVKGGNESCYEYFSCIYIPRSKLSYPKMIKLLILFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W469</accession>
    <name>A0A8S1W469_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0840210</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8185178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W469"/>
    <dbReference type="OrthoDB" id="668540at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010608">
        <property type="term" value="P:post-transcriptional regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF12">
        <property type="entry name" value="MATERNAL PROTEIN PUMILIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PUM-HD" evidence="4">
        <location>
            <begin position="55"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="115"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="187"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="224"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="16"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="25"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8185178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="391" mass="45486" checksum="17896B2CC792C4E8" modified="2022-10-12" version="1">MDPENDQEIIDSIVNALESDDDRSFSPSQTRSDTNRTYSPQIKRLQQPQYSIKKIQSPIIGFKLDCWTIQPFNLDDDQYLDGDLILKCKDQTNSRKIQGLFEKGNDEQREFIFQKLLPGIVSLANDIFGNYVVQRILEQGNQLQRELIFELLSQQILVLCYNTYGCRVAQKLLEISYNTFKFDQIFNILSSEIRNLVLDTNGNHVIQKIAELVKFQKSEWLIEGVLGQIQKLSNDSHGCRLIQQILELSSISQLNNIYKELLSIQDELCFSQYGNYIIQILLHRGPSDVIYKIQNGIIKNLEKLSCDKFGSNVVDKSVNISVYMRKEILKVFIHNMNVFYKLSNNCYGNYVIQNFCKQLEYNQLIKLSYDSSQFNTQFGQHVYQTLQKLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W9Z9</accession>
    <name>A0A8S1W9Z9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0830205</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000083">
        <property type="protein sequence ID" value="CAD8184709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W9Z9"/>
    <dbReference type="OrthoDB" id="301228at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35381">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35381:SF1">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8184709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="356" mass="41547" checksum="4E0649BA61AAE68C" modified="2022-10-12" version="1">MQETNDQMLIIKVANSKPIQIKQFDTPLQIAHRFCNDNNIELRKCKTISNQIIRIFRAYIDEFKQLQQFSKYFSNQEPFKKKAQYSLEEIMNQAEKENSYYSFQQESQLEEKQVNSIPSIDIYKSPKDGIQHLSTGAHIDSKKLEDMSRKLYQNILSPTPDSSYMISYSQTTKNISKLQRQLQLNKLQPTRPLQKSQERLKSKSPHNSIQKSPLLNKSGYFDNGNKVQSSLVLQQIKQQALSTKNQQMGPLRQLSNSRASSIGRTRCKTEMFGDDKLLRISNKLFNLLDNDKDGYISPSQIDLSKISDDILNYLIGILLEIDHNKLRLNISQFYKLFEQHYEKLDMLQKQRFLSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WAS7</accession>
    <name>A0A8S1WAS7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0860112</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000086">
        <property type="protein sequence ID" value="CAD8186007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WAS7"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF30">
        <property type="entry name" value="PHOSPHORYLASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="65"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="397"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="397"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="419"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8186007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="428" mass="50061" checksum="B508F495320CB0C3" modified="2022-10-12" version="1">MKQQSNRKLFELRQSVKQSLFLSLKTERSQYPTETKRSKSITQWKQFTFFKQNQQSKITKFHDVYKVTKKIGIGAHGVVKICYKILDQLKINYAVKKLSCQNDPELIRTIIQTFNINRTLNDNPKIIKSYDLYIDENEQVAYWVMEYCNYKSLQSYMQKSIPDVVIQQIMKQLAQTITEIHKKGICHRDLKPDNILVRLHGQIDIKIIDFGVSKKFMVKKKNDKIYHEMWTRTGSLLYQAPETFLGGGYDEKVDVWSVGVILYQLLCGKFPFFCDSKLDTIELITDPNLTIDQNKELKLLQPLQVDLLKRLLNKNPEKRLSAEEFSLHPWINEISTSKSTSRDDLQINKNNEITTSVNLDATSIKIDFSNHIHYAHKQFQELIIQIKDHVIEDSQSRKSSYQIPSAHTSYDVSPINQIDETKQQKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WB92</accession>
    <name>A0A8S1WB92_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0850240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WB92"/>
    <dbReference type="OrthoDB" id="1305at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="408"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="463"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="502"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="415"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="463"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="502"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="517"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8185725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="735" mass="84241" checksum="8CA098D9B12E92BA" modified="2022-10-12" version="1">MINQNTESSKWDWVPATQTLEITINSNKVQIETTPSIVLTHPEGDAYPEPYVTLVIDTEKQLKYSEELGWNWHKSWNLPNIVVRLEIKDSQTQELLLSPKNLYAQLYAVKKVQEASRVILQDVGLRGTSKLELIDGQAYFSALRFASTSYNNEGVKFHLLMSVYIQNSDTEKPQILNSTISPPIFVDSRKSAANSEQKKLYLHAEPFTFDLLDKKYFKRENKRKTEQDVEIENDFESLFNYLTAPNIRHKVKHPLFLIIKFSSCVSLYYNPQTVEGNTKTIMEKLQSQLTKDIITKQLGAASEKQYYLFIEENNDQNKNKKVTEAIDFLNTGCLEIVRKEDQIPSKFVKLGDSAEILENYKQCFDQLLKIFKQLTQKFCESDSNESDDNYSDSEPQSAQKKIKLNEYQNQPSASKMDKQEQEIKKPSIPPEQNPTQLQQQQLTDIAIQQALIQQQQQQQQQQQQQQQQQQKPIQQQPQPPKNKPTDIINKPIIPQAPQNLQLQNKKQVGQSSSFTAPQPAQQLQQQPPPIQHQQTYQKPSHIPGIQQINIPAQGLQGAPQHMNPAFPYQFQQPIDQNALQQFMQYNQLYQQLLLQQQQQQLNQQSYPPQAGMMGNPMQSQLLNYMQSMGGMGLPQGQMNPQSLMGQNQNFQQLQQQLLAQQLSSQLGLQAGLTQQLGAGMGLNQLQQQQYLQQLQQQMGLGNFPNFNPLMGLYQGQQHNVKKPGEGSGIIKQEPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WBS9</accession>
    <name>A0A8S1WBS9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0850075</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NFX1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WBS9"/>
    <dbReference type="OrthoDB" id="6512771at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06008">
        <property type="entry name" value="NF-X1-zinc-finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034078">
        <property type="entry name" value="NFX1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005062">
        <property type="entry name" value="SAC3/GANP/THP3_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000967">
        <property type="entry name" value="Znf_NFX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12360">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR, X-BOX BINDING 1 NFX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12360:SF12">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR NF-X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03399">
        <property type="entry name" value="SAC3_GANP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01422">
        <property type="entry name" value="zf-NF-X1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00438">
        <property type="entry name" value="ZnF_NFX"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="144"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="63"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8185419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1280" mass="150542" checksum="98ABB3159805D578" modified="2022-10-12" version="1">MSKQYNKRGNYQPRQDVYEYVTKEEYEQQQIQQNKEQQQRQDNPKKKQQQFQKNKYEQEKQEQFQQQQNQNKQSDNKQYQKHNNQKDNKNYQKRFQQKVEVDDDYKRDDPQDFEDVEKFDITKIKQKELTQTLIKMLKYRKIECPVCYDKIHPQQKIWSCTQCFSPFHLYCMHKWIKNLNPKNINELYCWSCPKCNLLIQDKLPEYKCFCGKQQDPEADSYSIPHSCSQKCQKKRGKYCPHPCPMDCHPGPCPECHVQGVEIKCFCEKKTKQMQCSEIKKDFSCGQPCGKMLNCNNHFCQKPCHNGNCQPCNESHQVQCYCGTQKDIINCAQKSYSCGKICGRILDCGQHQCLEQCHPNCQPCKLKPELVIYCACGKYKINDLIKEQRKSCLDPIPNCGTPIETTLICGHKSRTICGSDYPECVQKVKEKCRCKDTTRVKLCNDKSIFVCDDVCKKRKSCGVHFCQKECCPPGDQEAHLCLKVCNKPLPCGQHNCDQFCHIGACQQCPIIINQPLYCPCGKSVKNPPIKCGTAPPSCLEICKKVLECGHICQSLCHPGPCPDCREQIDKLCRCKQNTLSTICSKQAVCRNVCNKILNCGHKCQEICQAQEECPGNGPEGCDQKCHVKKICNHFCQEICHPNQQCPKLPCKIQIRVVCSCGNRDSFIECGVVDELIEKTLKCDQKCQNIKRFGAFYKSNSLVDIEKTYYPDILLKYTHYNLQNLQKMEEKVEHFLVHSKPEQGYDFSFPVSEWSELKKLALQTLFSRHYKFDVQILKTQNKQYLFSLYKTQDTCIPKIKLSEYFIKVQKGIIKRDHQPFKAKIKTFFEVTSTDAYNIENALSDFKGDFYSERQSQQYVLNFWDETQAELALKKIKKAVGLLNVNWVLEVNQQCLDEYKRIFEPAKENDIIVKPKKMKQQEYEENTLEIQYSQLEEEKFNKIQKEYSEKAQLIKRCDNEEIEERINNQQLSIFEQDGFYQYEIQPIRENIIRSYKQEQKPFVPEYVRTPDTLCKTVDFLLQNFLRADDPDSDFKKPMKGDTPRDIVYEDIYEFIHDRLQQIRVELNYLQYEDMSIYVKIIRFYILSHSQLQDNQENLKTLCELLKTFKNDETLQYTLILSCFNEIPECQQFYSQMLELKEQENLSPIIQSIISLTTQINQKLYHKYFDFYHNQENILIKSLMHGFLNKIKIQINEQFQQITSSLEQDQTFISVNKLLEQLQIEDKNNLIEYLNKLGLNDLQFQISQSDSEIEEINISQIQLQLQKMKPYGILDLDRLALIIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WBW9</accession>
    <name>A0A8S1WBW9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">EXS family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0870202</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SYG1 (TC 2.A.94) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000087">
        <property type="protein sequence ID" value="CAD8186691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WBW9"/>
    <dbReference type="OrthoDB" id="9970435at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000822">
        <property type="term" value="F:inositol hexakisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016036">
        <property type="term" value="P:cellular response to phosphate starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14447">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004342">
        <property type="entry name" value="EXS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783:SF103">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 53 MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783">
        <property type="entry name" value="XENOTROPIC AND POLYTROPIC RETROVIRUS RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03124">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51380">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="304"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="346"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="386"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="511"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="536"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="582"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="9">
        <location>
            <begin position="1"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="EXS" evidence="8">
        <location>
            <begin position="471"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="207"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8186691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="874" mass="105654" checksum="AE0199BAF3D4B3D0" modified="2022-10-12" version="1">MKYTQYLLKNLIPEWIKLYINFKYLKTHLAVVTKLKQLLRKIKKLKPKDVYKQFKYDVVANKQLFEKLEQDRNHFMIVFDNETEQIYSFTEWKYRELLICFEKLDKQIKIMEQMAYYTYKEQIIVTWNMGQNIKEQQREDAIEITVYLKQKEELLETSFKFYNECEQLQSYIILNSEGIRKILKKYKKQELKGIRNKEIEIQYFGNVQQLQKRLKKYETKVNILKQNTESLMINHFYADDPSECRELIRKYSEKGQINLKTVFYFGFFAGAAIMIILIILGMRFDGLLDPNSDKVFNKVFPCFRGMALFIIYYWFITLDLAGWNYFNINYKIYLGFNHHFSTVQELLQRVSIFTAIFLITFLWYCIAVEDSLGDLSRAVQLFDIRYLPIICWVISIIYVFYPTTKYFNPQGKRWMYKMFYGALWGHFIKYESRYTFFTDQFTSMITSMRDFDYTICYYHHFIFLGNEHNGECNFQRRFTAAQASIIPYLLKCIQYLTRARDKGKFLFTDEMYNFIKTTIAMSVGILAYLTRLDIGWKYYWIAVACFCSCFEYYWDLKKDFMFFEKGTKYKFLRNDLGYNSPYIYYTLGVLNFFLRIAWVLTISPDMYMIIGIKNEMFVLCFGFLEMSRRLINNFLKMEKEHINNLRTLKSNSDLKFPFKEKKDMELEEINSVFDIESISTLPRISFGEKNKDQQDSNNLKLSEINPKNLLNQQDYSKEDIHSPIKMLQSPKHSMLSKQNKKVSFLNLQFPLQEEIQLQQQQSNISKKQSSIIDHQLQDIKIPRNISFENYLNFKQSLRRINSFRLTQFLEQPKDTQINIHDYIHIHPNMDLVEKAKREEIQMQQKQKILNNENKQYFKIIEQYYEKYEIKQKHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WHK9</accession>
    <name>A0A8S1WHK9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0910152</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000091">
        <property type="protein sequence ID" value="CAD8188461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WHK9"/>
    <dbReference type="OrthoDB" id="10512815at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8188461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="156" mass="19151" checksum="8DF5D9E15B00E62F" modified="2022-10-12" version="1">MKENNSMNAYMNSYQDCLNSFILYYRTREFIKKRNFQDYNLNDVTIINIGLQTNIINRNQINGSFYMRFLRKYAHFIVSCFIHKICLLNLQKQYNKYLELSKSNTIYERVEEITNQETNFQEENQQLMWSQMQQFKNQCKVIYIDFLLDMLQDKNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WI83</accession>
    <name>A0A8S1WI83_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0900095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8187889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WI83"/>
    <dbReference type="OrthoDB" id="307028at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8187889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="272" mass="31871" checksum="62FDA3BC75FF0A50" modified="2022-10-12" version="1">MSEQQSKGESQENSSLLSSQEEQLKKQLKPVELVNLSKGSSQSLIINEQTSIREERKMKQYLQRIQEKERQEEAATLKRKRSKPNPPLKQQDSQEQPTSSENKEEEVNQPLALAVQLNPLLVPQQSVQQTETQSSSTKLRLNPKLKMKEQHHIYLYLSDCQDKSFEGIQKLQDSATQFYSPQKQTKDQTSLNQINQEIFQETSYLIKNIKNQYQQYIEPIQSINTNDQKEFYNKYFDKKYIESVIRESRLQIHKKSGLEKQTSVGKQIPKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WRI1</accession>
    <name>A0A8S1WRI1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0980014</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000098">
        <property type="protein sequence ID" value="CAD8190985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WRI1"/>
    <dbReference type="OrthoDB" id="299333at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8190985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="96" mass="11299" checksum="3162EBC8EEB144AD" modified="2022-10-12" version="1">MISEFDFNYYPLQLYDLQIPLTSQIQCENFNENEASARKLRVSSQCLIQKKKIRRKQKRPSLLKIDETARDVKIYSLGPSQTNLECTNDDQKQIND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WRV8</accession>
    <name>A0A8S1WRV8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipid scramblase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1010166</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the phospholipid scramblase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000101">
        <property type="protein sequence ID" value="CAD8192453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WRV8"/>
    <dbReference type="OrthoDB" id="444338at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017128">
        <property type="term" value="F:phospholipid scramblase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005552">
        <property type="entry name" value="Scramblase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248:SF9">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03803">
        <property type="entry name" value="Scramblase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8192453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="330" mass="38529" checksum="8FE226EB59DA4D66" modified="2022-10-12" version="1">MQSPLNTDDSIGQNNFYQMADYIQSPNQTSCLDILETQVGIFVQQRFEFFQVMIGITTQCKIFTRVQTPNVFRVYAANDQGQQIEEKELFKYKKKSSACARLTLGPPYRPFEMFVSNYWCKDFQPNMDQLFQENSDRIVFRFDRQYACTCCCLNRLLYLIIQNKSLGYIIQPYYCFTLGCHIYDSQDKLKQIIEGSCCQCYFCVDVHAVINVIKLILIQKLQSKQLNQLLKVVAPMKKQIKGCCQNWANITDNIFVVFPQNASKEDKTLILAATIFLITCILRIKMVHNHQIESNDYYSIKSIVYHIQLIILSLNLNQQNPIKIVLFIDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WS70</accession>
    <name>A0A8S1WS70_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Palmitoyltransferase</fullName>
            <ecNumber evidence="8">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0980065</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDO010000098">
        <property type="protein sequence ID" value="CAD8191069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WS70"/>
    <dbReference type="OrthoDB" id="331948at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161:SF85">
        <property type="entry name" value="PALMITOYLTRANSFERASE HIP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="347"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="375"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="463"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="520"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="162"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="268"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="10">
        <location>
            <begin position="419"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="561"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="561"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="573"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8191069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="610" mass="71300" checksum="85B65376403D9D04" modified="2022-10-12" version="1">MKKNEIEFQDSSISIKRQPLLDRKKSQDKDQGLRKSSFYEGGLSQLKKSIVEINQHQQIDKHPSLQDLYDSLTLMSLQQKYNQLLKSVQEQEVQEIMAILDHFITITPKPKLREIYDQKQQTILHISCFKNLPTAVERILIYEKDNSTIEEFNIWINQKNKDSFTAVHFAAYVGSLETLEILKKYQCDLKIKNEQGQNALSIAAQGDQVSAMVWLYMQGLSYTEQDEKGGTPLHWATYYGSEFAVQFLLSWLQKSKEGRKIINQQDTEGMTPLHLAAMTGNQRIAKKLLYKGSLKNIRDKRNQTPAQTALENEYEIIYKILETNNCLLEFLNIRTSYKPASISWNQICSFFIIYFYCMIGSIIFVYPFFNTDAWLQYLSIASFLIGLIFYFLTMFISPGYVERNDDSRKLFLLLTQYEPWELCPECQIHKPLRSRHCEFCSRCVIVYDHHCPWLHNCIGAKNYPYFICFILTIFVNLIHLIILNAIFLINDYPNPIHNTHYPWFNNVTNLEALDISKKCVQSSILILCLLFLFPLAYLIYVQLDNLFRNITTFEKYRQEEQTNVKGSKDKKSQQTIQSTSSIRSQESHNLSFSNCIQMCCNNSKNTSYQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WYX1</accession>
    <name>A0A8S1WYX1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1040084</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WYX1"/>
    <dbReference type="OrthoDB" id="21449at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04369">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027353">
        <property type="entry name" value="NET_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45926">
        <property type="entry name" value="OSJNBA0053K19.4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17035">
        <property type="entry name" value="BET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51525">
        <property type="entry name" value="NET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bromo" evidence="3">
        <location>
            <begin position="86"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="NET" evidence="4">
        <location>
            <begin position="206"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="182"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="308"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="308"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="328"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8193435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="339" mass="39216" checksum="7C1EAF5F4C4D1778" modified="2022-10-12" version="1">MTTFCQIDDVFQVLLTSDQARVIAKKLPPGFSVNLSRQSKRDVKKVQQDISSDEDNKVIKKSYVAPYRAENTDEMKRCMTLLQKLKKHQLAAPFLRKLDNVNYPQELEYVDLQIVEQNLKNNVYQTATQFWVDIKKIWQMSYTMNSKGSQLYAMTQELEQHFNELYKELDKPYPQKQIIDQPVKADKQQKKPPAVQQTPVNNKKPVKQPSLMEQPMNMQEKRALVSNINSLPPEHLRGVWEIVSDGLKIQEQSDELEFDIDTLPVKKTRQLEKYVNTKLEYLKKQQSKKVAEETKAQDKTDRIVHTANTTYNYAPQPVAQQKQPDTGDSSFSSDADSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X0S1</accession>
    <name>A0A8S1X0S1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1070008</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000107">
        <property type="protein sequence ID" value="CAD8194491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X0S1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8194491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="108" mass="13318" checksum="D142EEC1836F644E" modified="2022-10-12" version="1">MKSFNQKLMEVQVKDFKQIFTRSIYVIKSLQQYVIYLNNYQLGYLNATILNLYIQWRIFLKKKGMKKILNQNIRMNDIYWIFLNIHQEIIVIFQHSYSDQRSYFLDGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X4G3</accession>
    <name>A0A8S1X4G3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Jacalin-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1130183</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000113">
        <property type="protein sequence ID" value="CAD8196853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X4G3"/>
    <dbReference type="OrthoDB" id="10389882at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001229">
        <property type="entry name" value="Jacalin-like_lectin_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01419">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Jacalin-type lectin" evidence="1">
        <location>
            <begin position="34"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8196853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="279" mass="32420" checksum="D805C2F79E833B1B" modified="2022-10-12" version="1">MLNKVPSQISFGIDSKESQKFDDNLQIRTGEFKISGFKIYFQDNKICGMAIIYLNKGNGRKIIFTDTYQNQKYTSLYEFDIPSNDYIQVIFGHYDEQINQLGIITYQGKQDIFGIDQGQQFNCMFMGYTFTGCNGTFTSRSIESLSFQVQKLPKDYICQNLSPLLDILYPNLDNNLIQNTELMNSKPLNLSDLTIQNKSERLDYSSKNQDQLSKSEAITIKEYKGFDFSKVPVEEKQKQQEIIYIQKTYKEGFTHRDIREIKKEVGMAIGIFKLITHHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X613</accession>
    <name>A0A8S1X613_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1120108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000112">
        <property type="protein sequence ID" value="CAD8196400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X613"/>
    <dbReference type="OrthoDB" id="287071at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8196400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="186" mass="21375" checksum="19ED52B2EB228634" modified="2022-10-12" version="1">MNKQFKVALIGDSRSGKSSLIRSLKGEEFLYETQETIGIDIYQRSLDFKNMNYTFKLYDTSGCEAYRLILNRLLSQIDAVIICFDASQDKYQEQITGWCHLIENELRTIPTFIVGNKIDIQNFSLISDISSKYTVFFVSAKTGENVQKCFNKIFMSLTPQKHSMIQKKPQNKQSTWGLLCSCCKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X7E4</accession>
    <name>A0A8S1X7E4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1140055</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000114">
        <property type="protein sequence ID" value="CAD8196985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="302428at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="148"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="241"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1303"/>
            <end position="1327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1426"/>
            <end position="1446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1657"/>
            <end position="1679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="778"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1007"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="781"/>
            <end position="797"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8196985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1717" mass="201097" checksum="63DF58C5D2AAE276" modified="2022-10-12" version="1">MEQLFKLINLLIHPASTQLQLKSIVVLIATINKAQELEIVKNFELYYKIEMNPILSIISYCFIIDSISSTQHILIILFFFIVHIILFLMFTYFYFLRNKSTTIQYILHTFLTYYQLLFQIPILYSALSFIYDQKICNLSDICTLEMRIGMIFIGSLNIIFTLILNTIHIYFGRCEKLMEDNYILTLDYSIIFNSIMQVLLIISIILKSIDILPELRFALILIKSLTYIITSINRINQFSYILVEIIAISFNVSIAFNFSIYELFLILLFLLYLSIQIQYRVVNFYITLKQQQNLVTLQILEENNEMSAVPKSKIILSIIKKNHICKKCKNSHQLVECLLRRMANTIINRNQLYVLLYCSYVANHAPLKALIRVILMTSDDIYYKIASHNLQQELYKRTQQFQKEAQVKNSIRNANEHLNSLDVQSAFNTSHSASLLFPIILETLNSKINFWNKLINGYNDIDHCLHEALKTTSKMLRCKKEFEDRFDMVNNKLKGSSSQDILSMRIIQIYQTGIYSNSFQAFQLEKAIDDLLKSERYKQDESLDNIQLIQNRLIILKSSLVRRRGELINTNSKQLSQFLCDSEDNVKLIKHCNQLMPNFLQSIHDQLMDNYLQNGHSKLMVHGESTFFQNIQGYIEPCNINLYNYFDSNKNDFLLNMILTKEQSNNETILFGIDGKILGFTKQFYDEVLKSKTKIDSSHMSKRTETTIQQMEIKELLLRQPLIQYYIPSITQQVEELRIQINSSSNYLMNNLRSYWIIPSNHNDCLSNSQLILSQFKRRSNGSQTQNNQRSYKSQTYSRYSQNSMNTNMDIYDDQEIPQEIRKQILIDNTLIMLLHPEVQESVKKLVEFDTQSQYMQLGLFYSLQFKVLKYKKGTYAYFMISIKEIKHLQLQNTNQNLTTIPSQTTQQQSVYPTQDMNSSDLFLKNENMDIPESQNGIILEIKMMNQLKSVKQYDNNDDMSNNNILDNSKSLEMSFKVKQSERSNIFDSSRQMIVQQPLYIKPRLLDKQCNQEFEQMENDMHGLKERQLELLDENEQEIVNEKFESNSQTLKKNSILGKLRIAQIQKKENAMDFASNPSRTSTNSTSKESLLIVQQLYYNTQLITPLKKIGFLLSLISLAILAVDIINVQLISSNLISQTEQVKNLRQPQDINYFYSSAMYQEWIQYVQSLQLVQLSPFMNKRNVDILAQMYDFARQEMINLAIQVPKQAQNLNINTVFNFKYIENGVIKYQDLPLQDFYQVIYQTAETSYRNNVGKQQFIYPDMLTTGIIRVNLWQIIDLHNQLIQIIMQNTIDTQSSVRSYFLTVMMAQLFCVMFVIGLQLKYWLFIDHITKSILFLVSRINENQSIDQINRLSIIKEQLDNESSNKWKLFNFGEIMFEYQDKKSKKVELKQAISIQSTQNSYRATSALYSRIQKTYYLNRTNVIITFLLAIIWSAFLMTGYLIHMSYNDNFQPSLTVTLKFVQFRHNMDSLALIAGLTKTEPLIPNYNLSFINQTLSTSLLVDYKDNLLPLINEIADVILKNANENNEKSIFDGVLNYDLCLSTTLDNLPVCDLSKQTLIYDKKDIYLDIIVNGALGFTAAFVKFINQEYDYEINNFKYSPNIEECRITAQSQQFQNFILQYFTDIQTAMRQFLVLFQQDNSNISQNIINIIQVYYYGFGFSLFAIYCTLSFIWIYKQQQTIQSLRQILVLIPVDLILTANIRSQAKEIHQMLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X7E8</accession>
    <name>A0A8S1X7E8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1130149</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000113">
        <property type="protein sequence ID" value="CAD8196799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X7E8"/>
    <dbReference type="OrthoDB" id="448960at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050776">
        <property type="entry name" value="Ank_Repeat/CDKN_Inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24201">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24201:SF2">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="186"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="5">
        <location>
            <begin position="378"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8196799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="473" mass="55117" checksum="7DA77FFC247E80E2" modified="2022-10-12" version="1">MDSILQAALIRFDMAANDGKVNAEQVISILQSLNYNPQDITKIVSQITADIDVKQFKELLTQKEEPKQERLTTSQIRQIEDEFRQAVKYGNVDKVLQYLNKYQNQIDLANKMEPQNRQIPTYDAVQAPAEYQAYLILKTLYEHGSDIHYRDAMQQSIMFYICRDGRCQLFDFLISKGLSVNEQDQNGQTPIFYAARENKVEILQRLIQNGANVNHRDKILDQTALFYSAKEGQLQACQLLIDAGCKISHQDATKKTAVAYAKKFNRKEVYDLLMIGAQRQKDEANQKRDDGHSKIEQKINRKKNKDLPKFQYKLMHIDDKGLSKEVTNEDFEKFSQEFPEIAKLIMNPDDNIDETMISSIKEDQMWDKIAKKILNQLWKLKQSVYFLEPVDVNRYQILDYYDIVKNPMDFGTIKQKLQNNQYKCFKEFHIDILLVFDNCVLYNGIHSEVGQAGIKVKQDYLLMLEQNGINKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X8M5</accession>
    <name>A0A8S1X8M5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1150061</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000115">
        <property type="protein sequence ID" value="CAD8197348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X8M5"/>
    <dbReference type="OrthoDB" id="295032at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8197348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="133" mass="15384" checksum="2264BDB1CBD214A5" modified="2022-10-12" version="1">MFGRQQLRSYPTESSEFLEYKTNNRMYAQVLATTRESKESALLSKLVTLREQISTIGSPRSTVKTSRGMSEHVGVISPRSFGRRLDEKLPMKELGEIANYFNTLRGEQAKTLSKNYVNEMMRLQQCIEKKLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X9W7</accession>
    <name>A0A8S1X9W7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1160131</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000116">
        <property type="protein sequence ID" value="CAD8197824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X9W7"/>
    <dbReference type="OrthoDB" id="312738at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035806945" evidence="2">
        <location>
            <begin position="17"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8197824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="185" mass="22033" checksum="3E17C38916E90DA9" modified="2022-10-12" version="1">MKQIIILALLLILVLGDQHQNKHNHYKHKKGHVFKWVLISIFAVGAFLIIRKIVRRRRHLKRMKKQGLNKLSETVVFGEPMNDNIQQPQYYAVPIEQYKQFKNWLNQQKQQQEALLQQQQQIQQQLLLQQQQQQQSAQINQIPQQPIAGYPIYQQYPQQVPQIVYPQILNKSHVEIQLPEVRVPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XBU3</accession>
    <name>A0A8S1XBU3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1190001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000119">
        <property type="protein sequence ID" value="CAD8198448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XBU3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8198448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="101" mass="11495" checksum="BD662636ED874659" modified="2022-10-12" version="1">MVGIALLPLRGLPLPGLQRCVLAMSLQNEPSSFQNDFLDHHGQHSSLEAAERIQNLQGSKKKTSWSTPIMISSNQKEFNFNLGQKKLKNFSQKRFNQKLWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XCA2</accession>
    <name>A0A8S1XCA2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1190132</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000119">
        <property type="protein sequence ID" value="CAD8198700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XCA2"/>
    <dbReference type="OrthoDB" id="286336at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8198700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="354" mass="41495" checksum="9B6046F140D68D04" modified="2022-10-12" version="1">MLFRQKEKVPDLGRDNFRDFMRREGDSCLQTQMKLSTFVHKTVPNELTQSQLSPLPQALDGSLKMIGNTWDAKHLGTFLQKSQFASVGGNTLKNTEPFQNSMKNQSFFFRSQQPRMIDDHVDYLHQQSINHIISDHPMMKSGTKYLQKKLLSVPDKPFDESKLNYVTEVGLKKPKEQFQEMLISKDNQQKFHQWRSEFQDVNRAYKKAKQCFKSGLYALDSPLNDNTELYKEENTKLREQEQQSVRSSLQRYKSLERFNASNPSIEFNNVAHKPFNSKQSDPLLVKSNFPSQNFEFHDQWMKRKLIEQQIDTKNRIFGETSQNKANPQRAVFLREQEVRGRNYNPVNLTRSVIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XDE1</accession>
    <name>A0A8S1XDE1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EML-like second beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1200007</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000120">
        <property type="protein sequence ID" value="CAD8198812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XDE1"/>
    <dbReference type="OrthoDB" id="338608at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="209"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="229"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="251"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="293"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="335"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="377"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="387"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="419"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="461"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="503"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="545"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="587"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="629"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="651"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="671"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="713"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="755"/>
            <end position="796"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8198812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="882" mass="97879" checksum="DAB4EC3CB7224725" modified="2022-10-12" version="1">MNCAQEQKEDLYKLLASTKNIDEKVFRVIIEMLRKKNNSDCLEFLLNEDNQKYYGLYIDKQQKLNDITNDIKKITTVMKNIGEHDFNKNNYTQKIYQKKKQYLINKISKEKTIINFFKFLVHLTAIDKKMIQCGSNSLHLLVEMKVDLKNQCFENIQIRNTSLIGANFVNCNFSGSQFENVNISGLNLNGAQLFYCKWTNLLINELHQLNGHSNSVQSVSFSHDSTKLASGGEDNSILLWDVKTGQQKFKLEVHSSSVRSVCFSPDATLLASGSDDMSICLWDVNTGQKIFKFDGHIDSVNSVCFSPNGITLVSGSNDCSICLWDIKTRKKKTKLLGHSDYVTSICFSPDGTKLASGSGDNFILLWNVKTGQSKFQLEGHSDSVNSVSFSPDGNKLVSASDDCSIRIWDVQTGEQLSKLYGHIDSVQSVCFSPDCTALASGSSDNSISLWDVKTKKQKFQLQVHSSSVRSVCFSPDATLLASGSEDNLINLWDVNTGQKISKLDGHSDSVNSVSYSPDGTALASCSDDYSICLWDVKTGQKIIELQGHSDYVYSVCFSPDGTALASGSADNSVLLWDVQTKKQKFLLQGHRDYVYSVCFSPDGTALASGSADSSVLLWDVKTRKKRFSLQGHSRNVTSIRFSPDGTTLASGSDDCSICFWNVKIGLQIYKLEGQNFEVRSLCYSPDGTILASGSSDNSISLWDIKTRKQKFQLQGHSRTITSISFSPDGKTLASGSNDRSICLWDVQTGKKSISLEGHSDYIQSVCFSPDGTTLASGSGDNSIRLWQIKTGQKIQPLNKDQQGIFEKLKIPFLNNSFSESVNSNFTILTISNQPPVFSAQGALIFKGEFINHFGLDLLQLFKQRGCCILESQSEFKQNTKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XEG4</accession>
    <name>A0A8S1XEG4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1220007</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000122">
        <property type="protein sequence ID" value="CAD8199520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XEG4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12621">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN CHORD -CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12621:SF7">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8199520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="374" mass="43938" checksum="1383215D89527B9C" modified="2022-10-12" version="1">MKIFFQSRIDLEFFVKYLLEYKKQFAMTSINFNEEERSNYINKVLNFRILDQISQNKQITQKNIKLQLRIFEFIQKQILIFRNQSFSHLLFYQKNKILNTIDKLDIFGVPISFLTNAKTSLQQSRLGGLITFLIGSISLTYYLFLFILLIDHQIPPVVNFKQQTISYAEYQLSDSIIQLEFQDFLGGVDPFRKKIISSLLNYIELLIQLSIDKPSKILINDGTFVLNNGNTGNDENLDMKQLLLVLEGCSNLTTVSGSYCADENVITEYLSKFHGFLFLTIYLNQFQRQIRIKKQYYTALEPSKPLYSQVMLKQQESIIDDGILFNNYQNYQFLNNYELIHQQVDHFFTSQVVVQIFKSNFNVGNPIKQQQQFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XLK9</accession>
    <name>A0A8S1XLK9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1300029</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000130">
        <property type="protein sequence ID" value="CAD8202076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XLK9"/>
    <dbReference type="OrthoDB" id="2136082at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="576"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="656"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="71"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="138"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="265"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="513"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="576"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="588"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="656"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="709"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="763"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="781"/>
            <end position="791"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8202076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="803" mass="97509" checksum="74A49541186AEEDE" modified="2022-10-12" version="1">MNVEERPGTSFTSKRQVQTEQRKRQQMNLGTLETEDKSTGMKIRPITQDQQRNSLQQKRQKMIPQAILYDRESLYEEVINAKQINNNLQRENQALHTKIKMMDEELQRMVRATPISEQVINPMESFYQDSKPMKQHLIMDLKKQLRRLQEESIKKDQQIQFLQKNTKAMKIKELEIELLSYQQEAQRLKALMGMKFNDSDNDLLRLRADYKLLEDELKQLKIKCKEYESKATMYYNEKLKKDKLNQYLKNQIESLKFKDPEYKKQNKITENEQQLKQDIDQKQVQIDTLISSLKAKDAQYAELDKRMKELEREQQQIIDQLERDRQQLREQCNSLKEEVSQQSGRRRTVQNNSFLENKLEILPEGPEIIDQKKKLLPCVTKDDVNLICKQIKFKLKAMKVPFEKIDNFIHGDVHSVIRIEELNEMLQAEPFLLNEVESLKVARYLIEDNDERFVEFSLLAEGLIARAKSILKNLIGKYNLLEKNEEDQLFFEIAIVLSKYRNSLEDFILRSKKKQELCSIEDLEEALKFVEQTFNSTQYEFLLLKNYELTQELIHINYKKLFELFYVDSQIQQKTKSESDLPKEQVKKQSNNEIQNNKDIQQQQKQEELLQQQLKKQKEEDEQRKQLQLQQEQERQRQLELEKEEEEQRRLEQQWKEEEEERKRQEEEERIKQENEAKRQKLLEEMKKQEEQENLKKQQEQQRQLELLKQQKEEEERQRKLKEEEEERRLQEELLEKQRLEEEQNRLEQEYEQQAEYEKEQNEQEENFEEYNDQQFDQQQSEKQISEPQNKSQKDYEDEDFEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XLX2</accession>
    <name>A0A8S1XLX2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1300100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000130">
        <property type="protein sequence ID" value="CAD8202216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XLX2"/>
    <dbReference type="OrthoDB" id="303968at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001808">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8202216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="403" mass="47259" checksum="DBA5E95C73207C6E" modified="2022-10-12" version="1">MNLNHPTTQIRYQNVRLLQITPAFFEYSAIQKSNKREVILKIQNSINSNVLQEETAKLIQVQDIEGIPEVVDYGNNNDQRYFLATQTLGPSFSQIMQTNGQMPLKNVLLIGLQLITLIQKIHEKNFILSNIVPCNFCMGSDIEDKIIYLKDLTTLQSKNDNKKVVKFPIKEYTFLSPVLNIDKGPSQIDDLYSLAYLLIYLNNAILPWQQFNQIINEKQFQELQNHKLKIIFDQQFLDQQTSIFREWFKYLSTLKSSQHLNYNYLKNILISKIYENGWKLNDQIEYKNEIMSQSSKSIVSIKSRSRGTSITKTPNQRMIETLSPILEVDKEFELAQSAQKQSEEFPIFQQFQNLKNFMEDQQQTENNLSSSSIEQNECQIWKKMEILDKMSKPMQMMNKFVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XQ46</accession>
    <name>A0A8S1XQ46_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1320060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000132">
        <property type="protein sequence ID" value="CAD8202818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XQ46"/>
    <dbReference type="OrthoDB" id="294538at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8202818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="132" mass="15565" checksum="9C57D192DAD36AF8" modified="2022-10-12" version="1">MDIIYQIIYQKVAKNQFLFKFQRVFMSKYIYKSRTYSKVSEDQKKKLLKLVCFFGFKIKDAAQQLNIKYAAAKSYMIFHRNNVMMSKHSINSQQVCQIAPLSLKKCKLTIISKIGGDVVQQLKFEYPTIKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XUU0</accession>
    <name>A0A8S1XUU0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1390024</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000139">
        <property type="protein sequence ID" value="CAD8204875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XUU0"/>
    <dbReference type="OrthoDB" id="283324at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.760.10:FF:000012">
        <property type="entry name" value="Eukaryotic translation initiation factor 4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF8">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0810">Translation regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8204875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="200" mass="23727" checksum="87CCF26CF785AC93" modified="2022-10-12" version="1">MGDQFYQLCSKWTVSEKFNSSQNLEYKKTIVDVCSFKTIEEFAYLMKKTIYSKLSDVLSEPQKCKIFKQFNDNALDVQIEAIQFFKNDIKPWWEDENNKNGGEIQFDIPTQFYNIYDNLYQDILYEVIGQSNEDLSHINGLRVVDKINAKQGNRIRIELWLDIDPKDKHESILKLDEWLVSLIQKYHIEQAQLTQVSHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XVD8</accession>
    <name>A0A8S1XVD8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1370127</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000137">
        <property type="protein sequence ID" value="CAD8204532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XVD8"/>
    <dbReference type="OrthoDB" id="306099at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000089">
        <property type="entry name" value="Cyclin, N-terminal domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0195">Cyclin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="2">
        <location>
            <begin position="113"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8204532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="341" mass="40472" checksum="10818A7C44D8C2CB" modified="2022-10-12" version="1">MQFFDITNLEKSSQNLKTKSIVQVPKSPKKKNQLNYMEMKNIQFDWICLNQEEFEIMKDENPKVDSFTIQEQFFWFQNEHTHFAYPLTIEHGNFIESLKKHNIDECKRHNLLCWMGDVMGSYESASNETYFLAISILDKFLQQTNFLLTNQELLAIGICCISLASKCKDVSPLTSQQLIELSSDKMDEQMLIQCQFNILQTLQYQVEVPNYFKIFDYIMRDLEYRYYKYISSDIIQNSLVSIKLKAIYKCSLLLLKFVTQYYDSTIFHQSAISYGSIFYTIKRMELLQLSIPTDLINVLQQIQVDQKIINTEIVLKYIYRLCQNGMSKKQQQYLRQTNIKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XVK2</accession>
    <name>A0A8S1XVK2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Leucine Rich Repeat family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1390115</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000139">
        <property type="protein sequence ID" value="CAD8205057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XVK2"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052394">
        <property type="entry name" value="LRR-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8205057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1079" mass="126845" checksum="9452E0C5DCAB3B95" modified="2022-10-12" version="1">MKDDYSELTTLGTSSPSAYHRLVILQKSGGSELYTDLTNERNVQQKNKKQLFQKRFQNKQEKNDSFEVQDQLLKMEVNDWQGFLEKEHHAIQQINNEDITKTEIKTPLTLKMKTNPTMVSPTNDRTNYFYKMKLGNKQLSVISTQDFLKTVRYSQKLEDYSNLYKQQPHNLYLSLNFSQPERVPKTFGLIQNTRSLVTANASRSIRNSEDGNAFSNAMLTQQSRQIQVMQLNHNQFTPKQLRDILLTFPITLKDLELQNCKLNYMHMEILMSFVGKNQIYKLNLECNNIRDLGTQIIMKHLLNNNTLQSLNLNNNQITDCISTSLSNLLKQTQRLIELYLGYNYLNASAGTTIWKAMYKNTSVKILDLSHNNIASLECAQSINKAISRPYNELLHVDLSFNKFTYPQAQIIAEALQKNETIYGFHFEGNQTELCVNPNGFLINNIVYHSQLKEKLTQLYKQPQYFKLLEEAKVEQTQIRREESLVMLYSRSRKIRSTKLNVQKVNEINKLDTCWICEGWQEIKFEWTAHKSGSLYNEPIFIHFDFEDYRPLLMTFLNNEFFFIKMCPPNKEIHYFFTNPILGVQQTAMDQNIKNMQIQSIPFLYNDEILVDGNVMDQVNILRTTNKQQLFDKYMPMVQCKPREPMAKFDFSPYLNIKKHRWSVESSIFKHFQPDTPQLIDECFEFDFQNSKVTRLVKDTELLEIKENLKILYRQLFHIYKYHASGSLTAPIPCIMIQDYIDFLVQTSLMDGYKTNDIDISFTSTTAAKDVQFPQAFDKGLVRCQLLEIMIRMCNDKYIRQGICTTMTEAINLVIKQVQDYFTKFDQNQMWRKSRLWNQKCDIILNDRLAMLKSLFKYICKLSKKDKQLCKYDYISVQDFKDWIVQSKLICDDLSERECYLIYLQSMITQKDELYSSKHYQMNFHEFIESIARLAEKLSIIRGDKAMDIDDRRAKDLTPKLDGFLLYVYLVIGNEMKQLLPPNDPDIRGLDKCMINDFKSLKQKLEDSFTDGDEPPYDPRVELPHLSSQITNLLGNTIGGKKQTLRQQLKQVKREEQKFSLINFVQYFKSIQQVHVDHDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XVL6</accession>
    <name>A0A8S1XVL6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1350116</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000135">
        <property type="protein sequence ID" value="CAD8203954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XVL6"/>
    <dbReference type="OrthoDB" id="291115at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8203954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="193" mass="24044" checksum="8E41053A2E8A42F2" modified="2022-10-12" version="1">MKYEYSDKIEEQTCKLEELITQIQQDLIEIEQYKSICYRKCQYCLQIHDIQNKSFKNCIQNLFQRSKLLINKNESNQNKNIIVNKFRSRKQLLLKSYKNKTKQTWLIQQIQKWNQFLQIYQKNKCYPGKMTIHQRWTFYVIKVYHCQYCFKEFKSFYQTQYHMNKFCRKRSFPFVEEEPIIKNKIIKTIFKQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XZN5</accession>
    <name>A0A8S1XZN5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1460061</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000146">
        <property type="protein sequence ID" value="CAD8207119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XZN5"/>
    <dbReference type="OrthoDB" id="2139939at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044688">
        <property type="entry name" value="SCI-1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34117">
        <property type="entry name" value="STYLE CELL-CYCLE INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34117:SF1">
        <property type="entry name" value="STYLE CELL-CYCLE INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8207119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="82" mass="10398" checksum="D1E6386774EE511D" modified="2022-10-12" version="1">MSEFQKIDEDDYFRLNDIFCIWLMKKQNTHFEDLSYKDAKKKFKKFCKRYNNQKLDPLYYEHDKLIEKYQSDIQSKHKWNFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y138</accession>
    <name>A0A8S1Y138_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1400105</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000140">
        <property type="protein sequence ID" value="CAD8205344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y138"/>
    <dbReference type="OrthoDB" id="288896at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008536">
        <property type="entry name" value="DUF818"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF81">
        <property type="entry name" value="PROTEIN ABHD13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05677">
        <property type="entry name" value="DUF818"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8205344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="637" mass="74680" checksum="7C725781B1DA096E" modified="2022-10-12" version="1">MNILVIATITLSVLSLLIYLICYIISESVVISIGITLFCLYYLGHLICTLITFPGSVKSLYYKFTLLSLSDYLVCRTLQNLTIIINSMNTNKYTYQIIENAMNFLNIQCRTLTEMKQKNDQQNKYHAQLKLLNEELIQIPKNELPDKISTEKKQRITSYCKQLQIFLEHHSYQMSQKNIFQKFISQEFLCTLQQRRIEILIQYSNIERHNLKTSQKETIDCLFIKNNENGSTVLFCNPNAGYYEYMFFDCDWFRFYTQLKFNIILWNYRGFGESTGNISVENCIEDGRFVAEYFKNKYNIKTLGVHGQSLGGMVASEVAYQMKLSFLIVDRSFSSLGQVAAKMFSSSIVRFIYNCIVSWDKPNYLSYYNYVGPKLIIQDPKDEILPYESQLQTAIVRCHFADSTIKYSFKHSYFNQESKQYLKYFYTQILPKKHVLQLCTSLQYIINLAIKINQAENKSESENNKKYIELQNNDDELDAAKPILQQIYSLFSLIHYCGNSLLDVMHRQANPEKIALFFGSCFAFDQQDDSEFEKCFRNFEVGCQAFLEHSNYYINWGNIRTHVKIIRDISRNIIDKFQQIKQNNDYSLEMTGYTNDSDLSKKNMLGELISVSCGHNNNLSREDIYLVQQFFLKNKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y3D8</accession>
    <name>A0A8S1Y3D8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1480125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000148">
        <property type="protein sequence ID" value="CAD8207797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y3D8"/>
    <dbReference type="OrthoDB" id="295643at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="154"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="416"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8207797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="727" mass="85440" checksum="EF46F785D3D08106" modified="2022-10-12" version="1">MHKKKIKRNDLQSAPEIIRTRHRPTNHLEIENLVMQDMRKKILNDEMNTLQFYDIVLPDQDEMITSLKDSTKKPIKLHQKVRRTDLENKLRARLSNEPGAKLLLQEIYEKLDEDPDNHSRTVRKKLHQEEQFKANQQYKAESDDQLVANLDTWYKGIKKRWKDEQEKLKQEQEKEKLAKMQLQDKSQSLQLLRRGGLNDRSKAVQGIPAFPKLIVESAKSEGEQSGFKQKLMGSLQKISAMKKIEMLHQDQSLVKQLDKYMQQGSTLTQQELAQIELARAKQQGEEKHDGTLIFDYTVEGLKNSLHTLIKMQLTQFQNASDRALFYEKQTNQILLSINSRLERQKNTVKRNEKDLETLRMENHDIHKKLRAIQDFIKERQEELRGINKKPSQQQNSPNIKNNASQVDSYMVIAVKQNELEAQIDQMKDNYNKFQQQMKLNAIQIEKLEQENSKLNKNNKALLKSIQGFLLELLKIGLDCRSQGLSWIIKAVWDSEEKIAEANFPPYLDSKARDFLIMKTRKTIELNDLFKKAHQLFSSYRESSIDTGNNSIVSLLSKNHFSQIDSLDIKKQHIEQIKQKQESQVSLLVQGLSETENKLIQSTLTHIFQSKETLNDIIYNEAQRRIAKMFKTEANQEQSIVFTELNNFDKDKLIKEYQQIQNDIEVKEMLFRKFEETELQRLVKEIDYKNYLARFNVEALQVLAALFGYTRAERELIKYGMMHKMFNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y4S4</accession>
    <name>A0A8S1Y4S4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1490047</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000149">
        <property type="protein sequence ID" value="CAD8207948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y4S4"/>
    <dbReference type="OrthoDB" id="289628at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8207948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="226" mass="26917" checksum="54DF666C19EDE92C" modified="2022-10-12" version="1">MRIFRQAFEQLGGSRSRTKSDPRQQEDMCVVPPEEYFKLDRPQPLTRQFFTMLSTLEQKIVKECTVERVNQVVQMYQQCIEFYDSRKDDIKYYFVDKIQNALYEMQQISCLKTRLVTEVQSPVNQKINNLSPILIEEDEHYIRNKKKEKSIKLAIQEQVIQVVKQQPEKVQGMVDEYHEKLDQHASLIKKSVNQQEHQFNERLRKRSINKKSLSRCNSSGILEQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y7T2</accession>
    <name>A0A8S1Y7T2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1580018</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000158">
        <property type="protein sequence ID" value="CAD8210125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y7T2"/>
    <dbReference type="OrthoDB" id="306423at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8210125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="199" mass="23274" checksum="E0B86AFFF059A907" modified="2022-10-12" version="1">MPTQQQQIIIISDDEECVEVPKIEKSIPTQTINTTQSQKQIAKLLDLDTLEAQGKLYDYESSPSPQLPKRVIKKPPKPLYRPQKRKVIKSRKEPIKHSYQKIKHVQIGAERQQLQYAQSNVKAKFIRVYEKQEDKFSKLFDQLKLNFQEANDEDIAIILNMVGKDYQKAETFINENGLFIQTYLQNYKDLSEDDSTIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YEV3</accession>
    <name>A0A8S1YEV3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1650090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000165">
        <property type="protein sequence ID" value="CAD8211883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YEV3"/>
    <dbReference type="OrthoDB" id="292128at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="90"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8211883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="318" mass="37785" checksum="94B34079D3A8B987" modified="2022-10-12" version="1">MQKSLEFDCLFNSKPAHLNCNEQSIFIKIQNEELKFHISLNLHLQWYYRNKVLEQCQIENYTLQSSSKSLSQLKDYLNSQVMYLGASEIYEELETMSKSDLKTHVIVQSVITGEKYFCKAYKKQKEYQFLQEVRILNKLKNYKNVVDIVEVYESSKYFYIILEYLDRKLEQDYTHEENQIVIKEILIILEALQQNNIVHGQIRPQNFMQGNNHQIKLIGFSKAKIEEKPDTTDMYGLHKVMLYLYNFDDQSINIDQGIYPLIPGHGTNFLKGLVNNTQYRINIKQALSHPYLKCDETDHQIVQEQMFLKFKHNFSNFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YGV0</accession>
    <name>A0A8S1YGV0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1620030</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000162">
        <property type="protein sequence ID" value="CAD8211082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YGV0"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07834">
        <property type="entry name" value="STKc_MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000040">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000923">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="16"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="367"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8211082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="398" mass="46189" checksum="32E47EB1D4490FB0" modified="2022-10-12" version="1">MNQIVSGIQFNVGPRYDVKKLLGAGAYGHVALAIDKKQTDPDYQKVAIKKLHLVRDEIDAKRVLREIRILRTMKHENILRLEHLIYDDSNKELEFGEIYLVTNYMEVDLYKIIKSGQNLTDQHFQYIMYQLIKALKYLHSANIVHRDIKPSNILATENCEICFCDFGLARQIEELEAEDNRCQNMLTEYVVTRYYRAPEVMLSSHEYSTAIDMWSLGCTFAELITRQILFKGTNYIQMIKLIFDTLGKPGEEDLGFITNANAKKYVSSLQTKQKVSIGSVIKYSNPQAIDLLDKLLEVNPKKRITSAEALNHPYLEPIRDPDDEPSFEGNLDCQFENDSTIQLKDLKALILNEVNIMRQKNSEPIINVQLEIEKREQITKLNQQKKQQLKQQQKQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YGX9</accession>
    <name>A0A8S1YGX9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1620051</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000162">
        <property type="protein sequence ID" value="CAD8211122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YGX9"/>
    <dbReference type="OrthoDB" id="298882at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="103"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="247"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8211122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="361" mass="43372" checksum="C5F0A3DED3B39254" modified="2022-10-12" version="1">MDKQMIFSFQTQKPKIREQVKRLEYFQQELSQIQDLSRQNRSKSSTDMHSITTADSRQYQGYYEEVSAFALPKKDIFSQSLNKIQINQQNKEIQTEILPKPKLEILQENVMQLQLQKEEIFNQIKNIKKENGILISQNKSYEIEINAQKNKINELINEQNEKEQFYQQTMQQQNWQIKQLTNELQQLYNKQQQLNNYENNNPIQLLFAQFSQVEQENIVQVLKETTKINKNERLQVIQDWIHIQDIKEEIIQLKKHHNIKLKELMEQIKILQNDQIIEPELLSTQDSLQLVSNILYRAIKSNEMMKLIKQNTRMYNLIEDLWNKLKFRLEHSNFSQQHIRTSSLNKVDLNSTNKKFSCRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YHC8</accession>
    <name>A0A8S1YHC8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1620117</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000162">
        <property type="protein sequence ID" value="CAD8211252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YHC8"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF274">
        <property type="entry name" value="MYB-LIKE DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="122"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="2">
        <location>
            <begin position="180"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="180"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8211252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="400" mass="48104" checksum="2236CE901ACD5E15" modified="2022-10-12" version="1">MIQQTQAHLPKEWIDFLEKIQNLDPIDVETVTQYVKVRVNHFPTDQQMTEYYEEVFSKSTSDVNRKKWHQQDKNLLIWCMTKHMMSQNRQELIPNDEDWEYVSRILCVDKKLVELKWISLLHSNLKISPWSKEEDQILINIANDHYYKNNWTELTIKFNSISSTQRYPKQIRERWNNVLNPNISRSTWSKEEKIQLLQLILSYGKKWSKIQGELNGRSENQIKNQYNGIIRNLKRFNVQESEERLLIKAIIENPDQKLSLTVTQFMSDFLAKKEASKSVESPQLHQLTESQIQKKICIDPVPQEIGITATNILFQEKLNQCKEVQQQMKQTSIQQNTYFSSSQPQNYSQMPMPTNQYYYNNNYNNYYHNYHQQNQQYLTYPNYNQVQSQMPYQQYHYGYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YHY5</accession>
    <name>A0A8S1YHY5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1740002</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000174">
        <property type="protein sequence ID" value="CAD8213093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YHY5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="86" mass="9872" checksum="4F426B63DAEE88B7" modified="2022-10-12" version="1">MIIITTPNRLHKSQTIQNKSNIFNGLVSIERIQNKLGIGQPFGKVKNQNKVGGQYRIDSNNQQNLLYYTQKKEKKQGFGQNQKVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S069</accession>
    <name>A0A8S1S069_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0020178</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S069"/>
    <dbReference type="OrthoDB" id="287169at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8133002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="85" mass="10356" checksum="203EA5392608FAF6" modified="2022-10-12" version="1">MSLQIVNLPEFSYKSNTHRSLDLENFFKEFEMKQFLEECNYSQQELYVECRKMKQFQQKRKLKLNHYINSYLESPSIISPVQLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S6R6</accession>
    <name>A0A8S1S6R6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0040097</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0598 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S6R6"/>
    <dbReference type="OrthoDB" id="10260024at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028108">
        <property type="entry name" value="DUF4505"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31449">
        <property type="entry name" value="UPF0598 PROTEIN C8ORF82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31449:SF3">
        <property type="entry name" value="UPF0598 PROTEIN C8ORF82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14956">
        <property type="entry name" value="DUF4505"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8135187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="160" mass="19282" checksum="3F3A3775DC18C75A" modified="2022-10-12" version="1">MNKIKYRMSVFNRDYFMFIDHKGFLYLEETEPKNFTSCIKDVRFFQFFYKNLTKNKTGRHADYRFVSKCWGEFNFVKVQSTPIIYNDIQLVDNQWKIIAQNGYSENFEANQLFIKDNLLYYKVSLNDLEFGILSTDLAIKLGDNINECNTFRWDNNVYQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SD60</accession>
    <name>A0A8S1SD60_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0050258</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="66977at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17917">
        <property type="entry name" value="DEXHc_RHA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001760">
        <property type="entry name" value="ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF221">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX34-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="76"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="392"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8136654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1472" mass="174106" checksum="DC41B451F44D4F22" modified="2022-10-12" version="1">MQMDEEMLVKKESQVEDIVRKKVRTQNDNLGDYIPLQQKQEVQIQQLSHLRKRVIKPLTETERKALPIYNLSHKILEEMQNNQVIVITGGTGCGKSTQVPQMILRHHTNTEFETPRTVNILCTQPRRLAAKSLAKRVASELDCQLGTLVGYQVGMDSQISSRQTQIQFVTTGIFLQKLVHDREGVFRDYSHIIMDEVHERDIDIDFCLIIIKNLLKYFKDTKLILMSATICSDKFANYFSKKSINLVDDLTYIQRVDRKYKYVDMTQENDVEIAIKFEGEEDHDIATQWGQSWKDMDRQVKLTQEQRELEQKQNELREIGQHFLDGDDDAPAPIIKVPMSQKYPIETYYMEEIHSYFNDADTAKLEPHQPLRFQTSLYFQDTPKIQQDSVQAMLQILEFLDSNKIKDTQGMQGAGAVLIFLPGYQEIMDIREEIYQKFGEDRFIIIILHSTVTIPKDFDDIQKRKRRLILSTNVAESSITVPDCKFVIDFCLTKEIIYNPKNLTEKLALQYCSKASADQRKGRTGRLFPGTCFRLIPQSIFKQKMTQYSVCEMLRCPLEIIILRLKKLYQLSMDNENKTEKLELNTIIDLKQVFNDPGRTLKTAIDPPSTKQIENAISNLQMLGALSYPNAQNSTIHITRLGQMMSDMPIDIFLTRFIMYCNIIGCAYEGVTIAAILSQRKNYFLHHFMRSQKLFFNSLYLYDKGNEDDLLIQLRVYQEWEIKFFNILKSTITQHELKRELKRFTNRNIGPLEKLYCEQRSIDPKNMREILNVKYELFMRLDQQFKNEPLDLSNPENQLKIKSAFCAAFKQNTFRLAKDERYDKKIKYLKDQGFDYTRTIMLESDNIKFDGVNIQNKELIKEKIAMIMEQFCDFHQLDRSQKRLEDQLKDILAYLDEQAQGRQGLPKFHFRSDLQLLTEIKAKSKFTKSWVQSVILLNNTIIIEFKEVELSKLKYVLKRLMHECELMKRQQSRKQPQKIFWEQFTLSNQVDIINFIEITQSLIFYGCYCRLIHQHKEINCDPDSINFISLDQNKILVAYEVQEKDSNKRNSTRQVISIDQNKYLLWSQILAMLFGVQVKLYHNNTKTHFIKAKVDDRDYQFDYFITEDDLQTINSIRQQIKLQILEQNYTDSGIWQKIRQLDKVRQKILYTSDERWKQLFDIELKDEILSFQESSENKQKRKRIQHFELEEQKADSLDYMNPIQIIRTDYDSEIPKQFDNEGIKIYVQIQQLYQTWKQNIVNTIKKQNFFLRMGNTTQICCLECNDYMTVGKTIGLSYISEDPDIKKQLDNDALEWAKRMVMILDVSSFGDYFNISMKVDQIKEFLSDQRIQSIFEKLKLIFEPINYVFITCNSGKHIIGFYPKIKQFKEIVQIGDIQKFYIIDQKLKLVYTSMEEEKFVFRKLTIDEIKKKENQAVQQRKLQNLKFTCKCCFVEQRYDEKGYFEGNEQAYSQHIKQESHKKAFEELQFTII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SEG1</accession>
    <name>A0A8S1SEG1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0070248</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SEG1"/>
    <dbReference type="OrthoDB" id="194443at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071164">
        <property type="term" value="F:RNA cap trimethylguanosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14741">
        <property type="entry name" value="S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14741:SF32">
        <property type="entry name" value="TRIMETHYLGUANOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase" evidence="1">
        <location>
            <begin position="148"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8138665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="457" mass="53507" checksum="68C6584C8E53010C" modified="2022-10-12" version="1">MQLRQLDEDDEEETCFLCFKISRNKQARRSSKTQTNDKMSMQNLSFDHHIAESRRQIIQDSIIKLTDSVLKISDYPKNKLFFLKNKASKEEIIAKITEKKFNINDLSGSTQSLDSQKSSIQEQLQQIEHTPDNVARYIAKRLKEFVIITDLGCGTGGNTVQLARECPYVIGVEIDSKFIELAKKNCQHSTVNVDLINADIFTLNNLKTDVIFVNPSLNIETLPQKDQIKHCNPDIKKILFNHQKNTKNFVFQLPPQIDISQLPLLLNINSQFGYFRQNYPSFFSIEIEQIILNQQLEYIVVYCGDVSDIKQSEIIKFLTKQFRKVQPDCNSIQKQHLFWLFELIFRNIGIQNLTYRTVEAIKQKKSIENFCKFIQQQFQINQDLYQIYINHNKNDKNDIYSASKQDNDQQDIDNMSPSNFSYKQIEVANMNYSYTNENVFEDEEIETQNSFVMNFKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SG54</accession>
    <name>A0A8S1SG54_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0070327</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SG54"/>
    <dbReference type="OrthoDB" id="299080at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8138816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="204" mass="24558" checksum="B9BE63D938D3A7A2" modified="2022-10-12" version="1">MDKNNPNIKEYILSQLGEDPTDEAIKQVAEKTQVLPILIKQWLLFYKRNNQSQNGIDLQQQKLESVPKFNGKFEQYYEIDDPSYEIKQKKQFISDNNQKHTIQEDKTIEKQLKEVEKEIKKRKKILKTDKKQTDPYFNELYASDELYQEQKINDNQQFNLKEQYINVPIYYMIKLEESLMEQAINIHQKIVKIIEAKYDYLNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJC6</accession>
    <name>A0A8S1SJC6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0100133</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJC6"/>
    <dbReference type="OrthoDB" id="302225at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8141471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="327" mass="39119" checksum="6C70A67A824C2FD9" modified="2022-10-12" version="1">MDQCKTYSHIRSISSCKSKHKISNKSRDLINPRKRSVTFNSDVDLIKSISCQENLLDLIGKVQKQKQDYLLSLQNSQMSLLPPLKVEEEEIKTSKQQVKKQTNKEYYEFTIPMDPYKKQLYQKLNIYGKFIEPTQYKKIDYQQIQTFESAISQIQFQECNKEKINKLQEQDTQKQMDKLNSQVDDTQRFNQQYYSNYNQDYDLFQEQQKMNKIINEALKFKQQKREPINASKSVKQKRTQMRNYLENQYQNALNSVMQGININTEEDNLFIRKKYQKRIKLNTSFNNSRITSQYTKKAISSQIIHPDRQDFVNKTLEIYDKCNTYLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SLA8</accession>
    <name>A0A8S1SLA8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0100002</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SLA8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8141223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="181" mass="21473" checksum="82B79993A8CF0AE7" modified="2022-10-12" version="1">MQSVEQYTEQVRTLTQQCIETFDRFMDESYDRVNGAIDSIYEQICLKPAPEQIRTKEQIKKKLVQQQKAEVVVQEEQEVFERQEDLLQILSNANQIMIDDDDPSSIFKKLLDMLKKEQIKDCVAYLQQLKNSDKQEKEQIKKIINILKNIKDHEFNLMDYSSEQLEEIKKDLIKKLQGQRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SLD2</accession>
    <name>A0A8S1SLD2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Alpha-type protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0090193</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SLD2"/>
    <dbReference type="OrthoDB" id="430683at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04515">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004166">
        <property type="entry name" value="a-kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052969">
        <property type="entry name" value="Thr-specific_kinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47763">
        <property type="entry name" value="ALPHA-PROTEIN KINASE VWKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47763:SF1">
        <property type="entry name" value="DUF659 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02816">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00811">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51158">
        <property type="entry name" value="ALPHA_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Alpha-type protein kinase" evidence="5">
        <location>
            <begin position="513"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="78"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="391"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8140620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="762" mass="91303" checksum="E3E3101D49935096" modified="2022-10-12" version="1">MENCQIEDNSKSNYNQLNYNPFNNPNNFPPYPNQMNMFPNLQNFMNNNIASLGMPPTTIQMIPPFTIQRLPSLLHTTILNKSQKEKQLQQQINDLEELLQDQEEKFQEQIKGYKKSSQLSCHLKCIIQDLEDDIFDQNKLIQTLQSDNENKQLRIDKITKEKSFFESQINDLQEEVGQLMRQNQQLIRKSVAASSFDQQLIQEYDQQVKKSKEEVKKLKDDNNKQKLLFEDQLESVEKELDKQIKLADKEHQYFMEARDEITKLQKVIKQKEEKIKYQEFQQMNFVDTLRQANKQKQQFEDQLQSVEKELDKQINQADKEHQYFLEARDEITKLQKVTKYQEFQSRDEITKLQKVIKQQEFQQINFVDTLRQVNMDKQKLEDKIKYKDFEINSMNDQIKLLQNQVNFLKDLIKDSQNGKQIKLDQNNQYKQLEQQMLWLNEKRNEDKQKIELEIQQNELIRKEKLVKERKKIEDMQKQAEINKEIHRENQGAIEALCKQGNFYKFEEQPNVKTVNTKFEVWRVEIKDEEFNKNIETIERIQCPQDFSKFKEGTWNCLRTEIPFAFSMMKDIFLMKKLNVNSDELYVIKTPIGSQPYKTMNDALMECKSHLICQKLMKKFITELQEQAIEKKKNIKIPSVIYNDLLILEESQNSFWIAERFFKGDFVKYNNNYGYINEDYKKELNKFAQSFTYYTYFISNFNYMVTDIQGVGNYFTDPTINTHKGDFDETDMGEEGQMMYLVNYDNKKALLTYDYLDLLGIYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SLS1</accession>
    <name>A0A8S1SLS1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0090063</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SLS1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8140416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="321" mass="37877" checksum="020D27C534CB5F65" modified="2022-10-12" version="1">MKLNQNEFKFQTDGDNVIITFTFLSYITSNKPDHMSQFTDITFSRIVLNDCHYQWKEKVQILPTKFMLDSQIRKSLQTSTNNEILPQQINKKIDINFPEIRDELILLLETTLEDARDKDLSDNNDKFAISEYADSIANSPPSFNTPQINSTKLQDLIKEKFKKRLSRAKKTKKKKKLVKNNYNVVFPRRNDSNCKSNQEPVMFNFNKDVQQNRDNRINLKWTHRYNVIQPPKTVNEILCYVSNIKLSNDIKTNFGNSTYDNFEIKDPIIVVDIVDVIRLYKQYLLAGKQFYFIMLFVKKQQEIADFADIVQKNEQEFVQLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SMF2</accession>
    <name>A0A8S1SMF2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0090398</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8141020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SMF2"/>
    <dbReference type="OrthoDB" id="292569at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8141020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="183" mass="21536" checksum="05A565E518DFB8C9" modified="2022-10-12" version="1">MSFVDFESLIAKNFDQLINYYEMRWTQQNFKNPSNYTPQQVMKIVKKYIDELPIQEVLILGYISADQKGLLEQIFPRAIDELAIVEKILGQIKVLYAITEQPLNSEINDPEWILQKPGVLQNKKGMVEMRNLKIVVMLMVKRVLQNLIFIIINRQRVILLKIWLMKLNSLIQYDLKLVIIVSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SP04</accession>
    <name>A0A8S1SP04_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0110046</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SP04"/>
    <dbReference type="OrthoDB" id="538223at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="170"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8142253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="290" mass="33255" checksum="A0FD5B217FC2E9DC" modified="2022-10-12" version="1">MNFSIRRFLKFLEKRKFKTSLDSFLRQIINTFMNLQSISKKIQRINLQDHKFNQEDYSSETYEMARYDLMKRMKENKRIIDFIKFLVLLPSIYIGSNALNFLVGMKVDISNQSFENIGIKNTSIIGGNLVRCNFNGSEFDNVDINGVNFNGAQLFNCQWKQIKINELNKLDGHSGRVLSVQISPDGNTLASGSGDNSILLWDVNSDKNYNQILAQFKAPLIQNNSLPESNQITILRIFQTPLFEAKGALILNGDFINDEGYDLRSLFKSKGSCYLDNQLKQKNKENCIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SPY5</accession>
    <name>A0A8S1SPY5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0100110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SPY5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8141429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="167" mass="19843" checksum="3655B0CD147CA485" modified="2022-10-12" version="1">MYRSGKQQPKPELPFYADITYWITFMLSIYFKDEKILHGIFSCLPLFMIVHLTLNRLQRGELGSTVMMLSLLAESIAHLFHQFAHDERFMNLYFFEHLICGMFLAMGNFIYVFEKRQKKEIFRKKKSYLKSKLPKPQSIIVFPIIASGFIQCYMKQKMIIQIGYCII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SYX1</accession>
    <name>A0A8S1SYX1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidase C51 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0140185</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SYX1"/>
    <dbReference type="OrthoDB" id="299748at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007921">
        <property type="entry name" value="CHAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051705">
        <property type="entry name" value="Gsp_Synthetase/Amidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30094">
        <property type="entry name" value="BIFUNCTIONAL GLUTATHIONYLSPERMIDINE SYNTHETASE/AMIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30094:SF0">
        <property type="entry name" value="BIFUNCTIONAL GLUTATHIONYLSPERMIDINE SYNTHETASE_AMIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05257">
        <property type="entry name" value="CHAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50911">
        <property type="entry name" value="CHAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase C51" evidence="1">
        <location>
            <begin position="32"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8145323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="205" mass="24255" checksum="6D4C4A3BA32C8667" modified="2022-10-12" version="1">MNYPAWGTVMGQPFGVIAYCNTGFTFDRNKIHEYQTYWPKEETGLARDVYIGFKYQCVEYARRFLTLNFKAAFQDVPHAHHIWALDTIEDCTKEEGTFPFINFPNGSQEPPKFGDLIIYPYSKEQRFGHVGVIINVNLEEKFVDIGEQNYEEAGWECPEYARRLIMKIENGQYFITNQRIGKPYDNWDIKEEIFGWKRINFNQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SZL9</accession>
    <name>A0A8S1SZL9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0130345</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SZL9"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001072">
        <property type="entry name" value="Rab family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8144677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="223" mass="25275" checksum="A6D297B778BB7624" modified="2022-10-12" version="1">MSNYSLMFKYIIIGDTGVGKSCIVLQLIENQIRQEHDATIGVEFGAKIIKVNSMNIKLQIWDTAGQENFRSIIRSYYRSAIGALLVYDITKRDSFHNLQRWMEEIKNNGNANMVITVCGNKVDLESERAVTYEEGWQFAQQHQLIFLETSAKQGINVQSAFYQSAQRILNQIDDGSIVLGQDPGIKIGGQYKKKEKDIKSINNYEEAVQLKPVETSNGHNNCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T0G5</accession>
    <name>A0A8S1T0G5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0130250</name>
        <name evidence="7" type="ORF">PPENT_87.1.T0130252</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome b5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T0G5"/>
    <dbReference type="OrthoDB" id="260519at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050668">
        <property type="entry name" value="Cytochrome_b5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359">
        <property type="entry name" value="CYTOCHROME B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359:SF14">
        <property type="entry name" value="CYTOCHROME B5 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="5">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8144480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8144484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="114" mass="12716" checksum="0B883DABC6827C90" modified="2022-10-12" version="1">MSRKFTWQEVGDNAKNGWIVIGDSVYGPQGFLDSHPGGPAVIRNKAGKDVTRFFNEMGHSSKAHDILKSLKIGEIDKNSKPASWQIDSDQRLVAITSVMLILAFAFVYFFVLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T2A9</accession>
    <name>A0A8S1T2A9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0150009</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8145808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T2A9"/>
    <dbReference type="OrthoDB" id="293437at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8145808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="641" mass="71591" checksum="FC78F35F67ACCE66" modified="2022-10-12" version="1">MQTQNSFDIAPAETMLLKDSQQKKQVRFRQALVAGLILAAVLSLTIVFTPKGESVPTYTLNQRSQYYSQDQLCVYQVSTGSTNCTLIAANTSTVTVDSNQQNTTHLIILDGLQVTQNTTYSNGTTAYDNLFDANLNISQELSKNLRLLQVSADGCYGPCYDEIPIVTFTTDNQNGQVIEIGVPSSLNGLMLQTLIATIQHLGPNVQETQIDQDQIINPYIIGKYQFISKRNTETSWFGNTKVTKQVTQDDCLNENFLIGDQFSQNQTTLLSRQNQIISSKISVNIVMDHSVQPQGADVEYSYKTIVSDDSNLILGNSNIDVKYTQLLSDIKSKQNIQTYSLATIQDRLIKNRQNKSLNMYEMKQKAEQHRLLIEETISNSSVHCPSNGLIDFQKTVLTFTVKGTKLQLDLALKGTAGNGIADLNIKFCVAMEGMCLFEIINSNLNYTGQPFRDIELNGMQNISLFQTDLVVQGFTVGVGADFSYGFIVQQNVQQNATIFSVQDIFLVPVLLTVYGESSIPDLVAVRTAVVADIFTGNVTSNVAFGYDQKIQSINSNQYTLNLNLDMEEFRAIVIAQYQHSELSTKEKCKRHLGFFKVCYPIPVAGLLGDWQNLYYNNWGFPVLVQNYTLYGVQSDCFMKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T699</accession>
    <name>A0A8S1T699_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0160166</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. TatD-type hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T699"/>
    <dbReference type="OrthoDB" id="6079689at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008296">
        <property type="term" value="F:3'-5'-DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01310">
        <property type="entry name" value="TatD_DNAse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018228">
        <property type="entry name" value="DNase_TatD-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050891">
        <property type="entry name" value="TatD-type_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10060:SF15">
        <property type="entry name" value="DEOXYRIBONUCLEASE TATDN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10060">
        <property type="entry name" value="TATD FAMILY DEOXYRIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005902">
        <property type="entry name" value="DNase_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01090">
        <property type="entry name" value="TATD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01091">
        <property type="entry name" value="TATD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8147016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="302" mass="34916" checksum="A007C546E22742D4" modified="2022-10-12" version="1">MQIKSKFRFFDIAANLTDDQFYGIYHHKKYHQPDYKDVMKRASDIGCEHLLIASGCLGDLIKARELCQLSPYYYTTAGIHPCRAAELNKNYQGYMGDLIDQLELALKEKKLVAIGECGLDYDRLEWSTKEEQMIAFDPHFDLAEKYKLPMYLHNRNTGDDFYNIMQKNRHRIVGGVVHSFTGSLDELQKILNLDLYVGINGCSLKSQDNIDVAKQIPLDKLMLETDSPYCEIRNTHTSSKLVKTQFQAQKHEKWKEGQLIKSRNEPCKIIQVLEVMSELLKIDQEKLSEICFQNSLKCFGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T7C8</accession>
    <name>A0A8S1T7C8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0190309</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T7C8"/>
    <dbReference type="OrthoDB" id="294974at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20558">
        <property type="entry name" value="CYCLIN_ScPCL7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012389">
        <property type="entry name" value="Cyclin_P/U"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF108">
        <property type="entry name" value="PROTEIN CNPPD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027110">
        <property type="entry name" value="PREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0195">Cyclin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR027110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8149801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="174" mass="20943" checksum="7582823EAFFC3772" modified="2022-10-12" version="1">MSYKIFILKQYLYMQRHNQLLNTIAQILDEIIKETDTLEIEQDQISYFHTIKAPSITLYNYLQRIAKYSNCSEECFIIALIYLDRLQEKHPYLVLNSKCIHRFLLISLVIAIKFQDDDYYKNEYYAKVGGVSVREIFVLEQAFLELMDHQLFITAHHYFMYQKKLLEYGEIEMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TAP6</accession>
    <name>A0A8S1TAP6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0170096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TAP6"/>
    <dbReference type="OrthoDB" id="423343at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF263">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8147702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="341" mass="39198" checksum="30EE9C8C5C3F08A8" modified="2022-10-12" version="1">MGQGCCQKMSHLPSIGQQPEKLYTQTYNESQSQFITPDQTQPNVISDAKFLFNLNNETLNLDLGFGSNVFFYEEHPQIENEIVLSTLQKQGIYKMPKEPNLIDVVIYPPIQDKNTKIIYVGGWKNYQRNGYGLQYWPSGAFYEGEWKDDKANGQGRLIHTDGDMYEGEWKDDKANGKGTFYYYDGAKYTGDWFDDLPHGEGREEWPDGTVYEGKFSYGKKVGKGIYLFADGGLYDGEFQDNQMQGKGVYKWPDKKVYDGFWIQGKMEGQGSLKWPDGKKYEGQFVNGKRDGIGTLNFQDGRQYNGYWKDGLMHGFGTYKDKTGSKQGEWYEGRFNLESLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TC32</accession>
    <name>A0A8S1TC32_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0180372</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8149024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TC32"/>
    <dbReference type="OrthoDB" id="1911237at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000479">
        <property type="term" value="P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000228">
        <property type="entry name" value="RNA3'_term_phos_cyc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096">
        <property type="entry name" value="RNA 3' TERMINAL PHOSPHATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096:SF1">
        <property type="entry name" value="RNA 3'-TERMINAL PHOSPHATE CYCLASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8149024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="228" mass="26675" checksum="121330BA38E7810C" modified="2022-10-12" version="1">MLTQKELYIQQINIILFENLIDSEFIKFLQIQNQMAQQMIRLINLQIQLQIHRFRLQLQLKEDLFIKIRGVYSGSKVAPNLLIRVVSQCRNVLMMIYLMFGFILIYKNHKKELNFRIDIDRNSLFISCDCEYSNEFNNPEKVGETATMRLLDEIKNIGYFDNTQQQYALLLMAISQRKVSQIKLGRISTQIIETLKIIGTILGVTLNIDDSVFSCIGCRLINLSRLTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TCH5</accession>
    <name>A0A8S1TCH5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0210084</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the inner dynein arm light chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8150925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TCH5"/>
    <dbReference type="OrthoDB" id="290958at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019347">
        <property type="entry name" value="Axonemal_dynein_light_chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13183:SF0">
        <property type="entry name" value="AXONEMAL DYNEIN LIGHT INTERMEDIATE POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13183">
        <property type="entry name" value="AXONEMAL INNER ARM DYNEIN LIGHT CHAIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10211">
        <property type="entry name" value="Ax_dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0243">Dynein</keyword>
    <keyword evidence="3" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="163"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8150925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="228" mass="26490" checksum="B30D64A71788B080" modified="2022-10-12" version="1">MEDFVPQLNSLVKYSTPVLVSVAGKNTGDKKDAYAKQQVRANSPGRQEKMITQEILQQNLPPKEYRLKNGQLWVQTVLSTPATRIEVVQLQQELDKRLLSRGARETGICPIREELYEQCFDELIRQITIDCSQRGLLLVNSIAYGMRKMIDSESKKTDKTNKIMFMERECQELLKGVEELEQKIQYTKKVETEKLQKESDNHKNEIEKIRAYNNTLKDELGRLLEGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TDI3</accession>
    <name>A0A8S1TDI3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0190197</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TDI3"/>
    <dbReference type="OrthoDB" id="296638at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="266"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8149614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1248" mass="149519" checksum="4C4B0DDF02DA6E7C" modified="2022-10-12" version="1">MYQDPQNNEEIILLQTIEKDLSADQQIQQICKIIGQQNQNPRLLIFLQELFKMLFNSYVQKDTLQKAFKVLNNILNKYKFRNRPIQTSDLSHYINQKYYEVLAHFYEKAFLKGSDEAFKHMNFIYYKKIARDYMQSPNCTNSAITKSILTKIDQIYSTFTGNTPQEFEMAVFECLNMQEQELLTQIVTDDQSNTPKQTNSLQEKFLFNLPPDLQEKYRSQNFTDIYAFKDYLVQEKQIKGIEKILGYIFVDKLNYYLNSYYFVNSAKKIKENKSDLQKQLLELEKDILDSQKDYYSFIDIPSVKYFYKSLKSKRVRNLKGQCFSFKKKLIEFIKQEINSCEVPKKFNIAQFEKWIEIYFSLYSELQRSTTLEEANIAFNIIQNVQQLILSFLRFRWNYLYLFCALIVEAKIDKEQLDTILDIAYDMMQNTNKANLNRTFDQILAQLNVKANTQITSDYFNKYGSNLGYNSIQILESLFPNINNKLIKVMLQINFNQKIKLINDLKTFEFQLIHDCSQNLKNFSKIFEKLNDSILEGEKEEKEQKAIFFKTFPFQYQSRLKFLLSERSNKQLAQQFFNTYSLNQKKLKQDYQRKELGHYQEIFQREEKIGQICEQYILKEAERQNQQGDIYKDLIPIFINNFVKDNPFVIFLPALFKGLSFFRPQKQFFFEKLERVRFIAIAFKDKDQNEINKFIEFMIAQKDLYTFEQQFQIAKNIYNFYITLPIFVGESSLCKPITESFPIFSQMKNISDYNSTTYNAICVFTSKFFSFDQKAVIKSEDQFRNSDILKKLSNLMNEKTQLEFNQQDTLSTQFQELKIYKAQLIKEVQQNNNQNFQNKCIEVFQRQASRQLIISEIIEYKKQFKLPLNEIEQFQWSKNFWIQVGNQEFKQNNDNLIKKKVEDYFKQLIGPSSLSQLNDKLSKDFNTFQDPYIKEVLKKQYPRYKEEFEKNFNRTVINTPQIKLSILQMTEIQKKLSIIRQDQKYKEFQIEDQSVLTFINEQFSLYMKQLLGKVFQVAITQKPGYEFKVFQNKSVSPNDNLMTKYKQNQELQGKFGPQKNSYLDIALLTSSENKNQLYYELCDYTPIQRQIEFRNFLIEIEPLQEEKLKNKIRKNKWKEEHPELEEVLRKKIKRGAELWNQVQDFLCKRENNDNMMDSDEESEKYKGQQQQIIDIVEDDQLDQKLQDVQRSFERQLLTGRNYIKTRLNNMDNDNPLLTQKEINVKTVQFVLEQHPYFKKSKILYKSYIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TGE0</accession>
    <name>A0A8S1TGE0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0200179</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDC50/LEM3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TGE0"/>
    <dbReference type="OrthoDB" id="340608at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005045">
        <property type="entry name" value="CDC50/LEM3_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10926:SF0">
        <property type="entry name" value="CDC50, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10926">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03381">
        <property type="entry name" value="CDC50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="615"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="404"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="667"/>
            <end position="724"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8150276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="739" mass="87646" checksum="EAC2FA22988E04FE" modified="2022-10-12" version="1">MAEYSNSFSKQFREDQEEFLNIAIKKWKFHKNFKRNLIIFIFNLQIVEKDVYYGSSCTTNQLNCEIPIEISNDMTAPIFVYYQLENFYRRNRNYFKSKSVEQLKGNVDTDLSNCGDYQTNSDMEKVKSYGGNTLNKSENAFPCGEIAYTYFTDTFKLKNSNGEFVEIDETDIAWESDREYNFKNPKGWEKFAWTNIEDEHFMVWMRTAGQGRLKKLWAYNEQLYSSDMVKSFFMTTTTIFGQKNMVLVGSYFAGAFICLCSIIRQEKKNNLIIQFINLQQTYMKADSFQTKFLAQQIKNKIDQKNYHSSFNLGTSVFTSKMKSEESIDKKLSVSQPMSPSYLKAMRALQDRIRTLEGENGILNEQINISQNYCTTAHVKSKQRNKVEKLLSIMTKNDVSQKDKNDEKDQKIKELDKKLKQSEIENEDKIAYIHSDYKFQISQNESKIRELTEEISNIQQQLNLNMDTNESYKKKITQLNQQLQKEKQIILNYQLQLDTFIKSSKQWNESMSKMNQDIKQYKEQIQELQQYLDWYTTQYPVDKFQYMELEIKQLQIKNTDLVNQLDQQRQQNSQLLSDIQLLKLQYERIESQKHKQIEQLNGKIFELQLLVQRQDSKDQHQQTSNKKQVKKFSITQVPTSEQEQIKQTTDDDNSFVIIKKNNEDTQEYENISRQIIKLELMLEELDQKYDQIVKQAQIETDMRVKQTLRQELIKILAQIKDINQQINLLISQQKSLKTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1THK8</accession>
    <name>A0A8S1THK8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH OST-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0220080</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8151740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1THK8"/>
    <dbReference type="OrthoDB" id="369831at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025677">
        <property type="entry name" value="OST-HTH-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025605">
        <property type="entry name" value="OST-HTH/LOTUS_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14418">
        <property type="entry name" value="OHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12872">
        <property type="entry name" value="OST-HTH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51644">
        <property type="entry name" value="HTH_OST"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH OST-type" evidence="2">
        <location>
            <begin position="376"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="HTH OST-type" evidence="2">
        <location>
            <begin position="469"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="671"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="671"/>
            <end position="703"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8151740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="710" mass="83718" checksum="6072E488486FF946" modified="2022-10-12" version="1">MQQQQQQLRSSEIRRQSSTQSNSAIDEPFLKFLAESNYLESEQSSQEKHQKLTKCKTEHSLQPIQYINSNSFTVSDKKFSFPANEILEWETLQITLPPEVYQFLLFMKTQCSNGTIVYTNEQIQGLLDDSKVIIKNQLELFQKLIDKGWISKTTRLFNDIAINFYALSVKKLTYENLVWIIRSIKRDLITPTERLVQSRIKECYGLKYTRIEWELIMNQLKSQKQQIKFRSSNGKLIELPLLQVKNIKDPLIQEETYGIYIYQQNWIVEDEFQPELDKKQEWTIFIEFLMDFFSKTNAQNNKILKGGRYGCAQFIKLLGPKKLRELSLGRLTLYVQMAVNKNYIRYNKTILIQESREKSEHTTDSSVDGDPQIKIRVKELQEQLIELLLENPDGVSLAQIPTLLKERVNFELNLIELGFPKLKNFIESVKDVISIENSGRNNFTAKLNYSKLPKKYQKVKQSESFNQKYIYTVLQMIKNILSKHKYGISINQLYYDLSQLLGEWFNYQRFQCQSFFQFLQNYAENILVIVCQKGNQYLIYERDLRFLPPPQPTQDINDQQFEQILSEAFQCSLGLSIPQSSFSSSWLGKVGDSQIRQKSPPRIEDSHQEIKDNLKFIDELLGNENKQNDTYSIQDWSEKSMNPLGMISSINYGHSEIQDFDLTKELQFSIQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQNLDIKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TL89</accession>
    <name>A0A8S1TL89_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0240357</name>
        <name evidence="2" type="ORF">PPENT_87.1.T1750007</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000175">
        <property type="protein sequence ID" value="CAD8213190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TL89"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8153685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8213190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="61" mass="7481" checksum="8D9F18A45D767366" modified="2022-10-12" version="1">MGFFRFSLFFVSLNQKLVRNIEASLLKIIIRKLINGEHHRKRLQDQEKVKRRIKQLYLKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TRC0</accession>
    <name>A0A8S1TRC0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0260179</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8154860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TRC0"/>
    <dbReference type="OrthoDB" id="295867at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035772044" evidence="2">
        <location>
            <begin position="18"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="233"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8154860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="365" mass="40280" checksum="9687A71DD67CA22E" modified="2022-10-12" version="1">MNKFITIALIALVSVQALDIDFALLLQTGSQSNDAVQAVYDLLNDLKTSNIEAQGVADEKNISDEEIGQGRIAALSKVNELNQKAWASAKARREQIGVEYREATDYIAWATQRLADIDRREVELEELRCFSNGLFVRAIKQHNDAIGVIKILKNDLSGYLTGAPSSMVELKVENVADKLKQYSQLFNQDAMSKFAQLAAEQASGNAELHALGESDASTGGDRQPGVNVGQLVYNALSDLEDQLKSALAALEQNEIAGYYQLADWLADSEAEKAHLQDEIQRKTQLQDKLVVQEQAALAVQAKANSVLKDSQNAINAATATLQELRDLYETELNRRNEENAIIDEVIHIFKQQVLEMANQTSFGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TRF5</accession>
    <name>A0A8S1TRF5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UBR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0270024</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TRF5"/>
    <dbReference type="OrthoDB" id="320381at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003126">
        <property type="entry name" value="Znf_UBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38924">
        <property type="entry name" value="ASPARAGINE AND ASPARTATE RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38924:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_10, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02207">
        <property type="entry name" value="zf-UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBR-type" evidence="4">
        <location>
            <begin position="34"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8155315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="113" mass="13202" checksum="CFACFE33BF36BC19" modified="2022-10-12" version="1">MKDFQLVQHVLALSDNEFQNSRIQLKDTGNNKICSATLSNNTQYYQCFDCCKEPNHVYCQECFMPEKQQIITIFILTLDHVVTYDYLKFGFCDCGDVVKSLFLLINKYLSILH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TVY4</accession>
    <name>A0A8S1TVY4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0270206</name>
        <name evidence="3" type="ORF">PPENT_87.1.T0270208</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TVY4"/>
    <dbReference type="OrthoDB" id="300909at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8155617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8155621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="172" mass="20260" checksum="2B1BF3EDA58DAE80" modified="2022-10-12" version="1">MGGCLYRESKDQYHYNTEDILLKQKLTHDRLQNLLKLREDREQMQQRYQKNIEEGEKMKQPKKLNQSSVEQSGFDNSLSKNHQNLHYIEEAEDESSFIEQQKPQTLQKLPDEDNYDEGGELLLQLASSQQLNANKADNIRDQLQGRQSNEMDDEILQYFNTTKANYDSRVDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TVZ8</accession>
    <name>A0A8S1TVZ8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0270211</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TVZ8"/>
    <dbReference type="OrthoDB" id="297260at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8155627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="322" mass="37160" checksum="3D95EE739F76162F" modified="2022-10-12" version="1">MAQHLQDSELLTAKPTLHNGQEMVIQGLGTQEQSDSSFSSQHLCSSQQSSHRNINLSQSSFKKISKSSSDLQNFSNKHAHICAHYNFLGRCPLEHCKKRHIFISEDDGLSELISDNIDLIDNLTNSGNKLKLPHSTYGTQLSKYRKCKSNTKEFCEKLEHKFISQEQIHFGDFPIQNFWNWNAFALQSFSIKSQPDLGNDPQENKYFSQLIKPKPLVDYKQASEILENSSSALNISNEIKKNLMSKLKSDIDKMQQKIKTISMLKKWINNQQVDQNEYKNLYSSLSQIQQIHYSNFLRITNISISNQAKFNNQFQNVEYVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TX11</accession>
    <name>A0A8S1TX11_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PARP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0280297</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8156383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TX11"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001510">
        <property type="entry name" value="Znf_PARP"/>
    </dbReference>
    <dbReference type="SMART" id="SM01336">
        <property type="entry name" value="zf-PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50064">
        <property type="entry name" value="ZF_PARP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PARP-type" evidence="3">
        <location>
            <begin position="5"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8156383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="134" mass="15894" checksum="B54C787D49034A1B" modified="2022-10-12" version="1">MKQNIEVGYAFSNRVKCHFQNIIKDDIRIGHVLTRQPGLSFEKKLWYHLNSLYSIKIDRNQDLDILNIHGLKEEDQKKVRKKVDQIRKFYKCCFKGLKGSEIFAKTGKLLELCKNLKRLTINSKKKQCQQAMFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U1A8</accession>
    <name>A0A8S1U1A8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0320288</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8159045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="311768at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="153"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8159045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="449" mass="53290" checksum="06B54B0BA592207A" modified="2022-10-12" version="1">MGQIFQKHATQFEPSLIVKLKSITMRELFDCVDTIDKYYPQNCYLNLEQFDDIFATLTDNCIDLFHRLADQTGEDGKLSVHTFEALAAFAIFSGESFETKCMFVFRLFDFDLSNTLEEQEMVSTLQCAVRAMCKIAGLVIPSVSMLEKLGYVCFQMMDEDKNKHVDFDEFYEWTVQYDELQEFMLLYSGTQTYQYALKRMINVMDESEQQFNKWLRECKAPQVPAIKLQQNLYQIYKGIDQLSIDTLFYMIIEYTLRSKSIGQEDSSQQQKDLWAWKADHKKNQHLLQNISVKKSDYLDIIRAWNVYSITDVNQLSIINIKDLKLLIWLYEDEEPDYFRINQEMSIIDTNHNNIIERCEWMQYLCSEESMQRRQTPQVLMKKVFDNYDEEQCGLIPIIHIDKLLRDTFKQQMKKMKDFKSIQSVNSLIQQXYDGYEVSGEWKGDKFYKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U275</accession>
    <name>A0A8S1U275_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0290158</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U275"/>
    <dbReference type="OrthoDB" id="290938at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="295"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="305"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="322"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8156766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="359" mass="41771" checksum="996D33533990E0BF" modified="2022-10-12" version="1">MQQLQNCLLDKEMILQSDGTLYIGSFYQQHFEGYSLVINPDGSYYYGLFKKSKPKEVGVYKLKNSCLKIFWGDDNQIKNVKDIGNVGFLQKIISFKNNCYGNKIFIKINPLSYGVAKNQKYDGFTVMHYKNGDIYYGNIQKGKQDGEGIYYNNNIQEWQWNKYTNGKLISHQLYGKNLVPRELISKLFNKLNFKSYQITMVSLSNIQQLIINQLLLNNEQNHLSLASTVQSSHPAHRENLSQILIDDEDNSNNEILEILQSSQIETIDQELHIYNQNNQKDEIEAKLSKCQKEKAQQRIEKQRQNKFLTQPSQTKSPKNSRQQQMTLSTMQSTQKTTIYKRSVSPIFTTVRHLFSVIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U372</accession>
    <name>A0A8S1U372_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0290331</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8157086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U372"/>
    <dbReference type="OrthoDB" id="10267058at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047429">
        <property type="term" value="F:nucleoside triphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00555">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00528">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003697">
        <property type="entry name" value="Maf-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00172">
        <property type="entry name" value="maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43213">
        <property type="entry name" value="BIFUNCTIONAL DTTP/UTP PYROPHOSPHATASE/METHYLTRANSFERASE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43213:SF5">
        <property type="entry name" value="BIFUNCTIONAL DTTP_UTP PYROPHOSPHATASE_METHYLTRANSFERASE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02545">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006305">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8157086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="214" mass="24560" checksum="4BF12B85A7785073" modified="2022-10-12" version="1">MLLTYKEFLKNAKIILGSQSKGRQGLLKMIDLFKFEVFPSTFEENLDKQLDPTTYVQKTCEGKVINLLETFKQNNQEWDICIFADTVCEINGKIIEKPNDKEEASNFLKLFSNSLHLVHTCVYVVGKNEKNEIFQVNGIETTEVHFQYLPEKSIQTYVDDESNWKGRAGGYAIQALGNMFIKKINGDYSNVIGLPLPILDKLMSQVIEFLMKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U6G6</accession>
    <name>A0A8S1U6G6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
            <ecNumber evidence="3">4.2.1.93</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">ATP-dependent NAD(P)HX dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0330071</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(6S)-NADHX + ATP = ADP + phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19017"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(6S)-NADPHX + ATP = ADP + phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32231"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.93" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U6G6"/>
    <dbReference type="OrthoDB" id="8110916at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047453">
        <property type="term" value="F:ATP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110051">
        <property type="term" value="P:metabolite repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00196">
        <property type="entry name" value="yjeF_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592:SF0">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51383">
        <property type="entry name" value="YJEF_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YjeF C-terminal" evidence="4">
        <location>
            <begin position="3"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="165"/>
            <end position="171"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="226"/>
            <end position="230"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="245"/>
            <end position="254"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8159317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="321" mass="35824" checksum="838683320460D8A2" modified="2022-10-12" version="1">MFKQHSFSKIIPLLDKTRHKGQNGKIASIGGAFEYTGAPYYAAISSLKGGGDLAYIFCTKSAAIPIKSYSPECIVYPYLLEEGEEQQLETAINKLLSSTQIMHSLVIGPGLGREQITSKVLEELFKQNNSIKILDADALWHISQKKNKLINIIKDKSDQFILTPNAMEIKRLLEYFEIEYIKPDYNFINVLNDQDVNYKQIGIENGYPGLMAELSRKLNNVIIVSKGQNDIITNGKVGYAINIEGSKKRCGGQGDILSGLIGLYSYWSQDYKIDKIEGCILGSVVTRRAAQMASIKEHYSLTTPKIIDHIGDALYSIVQDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U6T3</accession>
    <name>A0A8S1U6T3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0340269</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8160304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U6T3"/>
    <dbReference type="OrthoDB" id="298281at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8160304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="213" mass="25339" checksum="9BEA7B7831536829" modified="2022-10-12" version="1">MSLRRMRSGGVHADSEQDYLNKISKLEKQRTYVEETETFEGFIMNSQFSFTCFGDQQMLKTTQQSHFILIMIDDLALSPKRLQNMIENTDNCETLTQSTNDDQYIWSSQSMNYDCVVHNYTVGQVNHLFNIWLINTPAMNSTFKYCYFRLFEAFICVNNERNEVFKEIYNEMCKYNEKVQQFHIKENDEVSPERKQINNFQQILKILIDSKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U700</accession>
    <name>A0A8S1U700_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GTP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0340294</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8160354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U700"/>
    <dbReference type="OrthoDB" id="26136at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990131">
        <property type="term" value="C:Gtr1-Gtr2 GTPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006762">
        <property type="entry name" value="Gtr1_RagA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259:SF2">
        <property type="entry name" value="GH16429P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259">
        <property type="entry name" value="RAS-RELATED GTP BINDING RAG/GTR YEAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04670">
        <property type="entry name" value="Gtr1_RagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8160354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="305" mass="35381" checksum="417333C21AACBF6D" modified="2022-10-12" version="1">MNNDICMFGLTKSGKTSMIRVIFQKLEIFRTFQLDPTNRMESVTVNLGSHIHFKIYEFSGHYDLNDSQPPEIAAMETCSLMIYVIDSQAEPFNEAVQYLRQAIQTVKQRSPHCECHCFIHKVDPDIEDNKKNELLQQIQKDISDELSKNSMNYIKVDFHVTSIFDHSYLEHFSKVIQRILPYSQSIQTLLDSFNLSCKIEKSFLFEIYSKLYLASDSQHFHSTNFQLCSEMIDVFIDVTCIYGKLDDAGQSQIKLSDGSILIYQTVNEGISLISIMKSENLERPFLLEYNINQFRQGLAQIFKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UC77</accession>
    <name>A0A8S1UC77_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0360254</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UC77"/>
    <dbReference type="OrthoDB" id="299061at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8161573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="97" mass="12092" checksum="6BDAB54A95BA9379" modified="2022-10-12" version="1">MHYLSEKFYGKLDTNVYYYYQVKNENTNYCASFNYQCFFLNNLLFNNYVDHTQDKLKPKLMQNVFEFVEFLQQNKKAYFKDSSRRELEKSFEQYYQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UC85</accession>
    <name>A0A8S1UC85_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0380279</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UC85"/>
    <dbReference type="OrthoDB" id="302162at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="294"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="198"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="244"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8162861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="317" mass="36855" checksum="573B22E9587145D1" modified="2022-10-12" version="1">MITVPQGERLECFKIDGLFIGTLTLNNTKPNNCCAFKIRTNNTQDIQVMPHIGSFTGQYIKIIVKCQKFQSNAKLQIFIVDLTDQNFNKTDVKSLASYFDSYSNSTFVQKMKIEIITIDQSQMTKQSNIINQSISLNKNIEESIYHQQDFTYEQNTLLKKNEQMKQQQIKNSITNSDQHQSVSRVSASEQRFLTSTTFQQIKQTPSKQSSNSQNNLPLEYPLPKPQTYPYQPQQPKLYNQQVYEIEEDNQIQRLNLKINQLQIELNKQKEELQNIEKEKILLLSKGTNQNQDQIFVSVQHIFFSALFCFFIGYVATN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UFR2</accession>
    <name>A0A8S1UFR2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0390133</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UFR2"/>
    <dbReference type="OrthoDB" id="270720at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF179">
        <property type="entry name" value="OS10G0565000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="22"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="62"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8163264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="377" mass="44056" checksum="306D68510CBF3107" modified="2022-10-12" version="1">MGSQCSNCQSCQKKDEILISEFHQQSMNHYSNNQQDCEEDDEQENTFKEEQKESKQPIQNSVEHEVQNQAQSEKKMEINLTSQSNSDSQRKKNEMAFRNHEDILETLGSRSNCKQRERRSKHIFKSGASYEGEWIGQQRDGYGVQIWVDGAKYEGEWKNNKADGKGKFWHLGGDYYEGLWKQDRACGKGIYIHANGAKFEGEWLNDQPHGYGIEVWVDNSRYEGNYSHGKKDGFGKYFWNDGSIYIGNWQSNVMDGYGVHYWVDGRKYEGGWKKSQMNGRGTYNWPDGRQYNGEYLNDKKQGYGVYVWPDGRKYEGYWVNGKQAGKGRYILPNGKSQLGLWEGGRRVMWLDQDEDIKPLGWDDYEDPKQYYINHAYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UIA9</accession>
    <name>A0A8S1UIA9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calmodulin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0410302</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UIA9"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000003">
        <property type="entry name" value="Calmodulin A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="6"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="117"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8164921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="154" mass="17443" checksum="8556F05EE58DCD7D" modified="2022-10-12" version="1">MNLTEEDIDMCQRAFAEMDEDGVGAIRGQDLKIALEKIGFKPSENELYKIISEVEETNTGLIRFSDFLGVYWKFKYSNQDDDDQDTIDAFVAMGGNPDKTGSISTDKLVQIIKSEFELTIDIESLIRDVDKDNSGQIEFGEFKDLLKTSYANDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UJD6</accession>
    <name>A0A8S1UJD6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0400107</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8163872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="318898at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035907167" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="16"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8163872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="499" mass="58692" checksum="BB54A8360AEC1788" modified="2022-10-12" version="1">MIFLSSFFITLYAQALHSNYNVILTQGETYSLYMKELFKNQTQNFSIKDCPDNVYLINGVNKLSEQNNMNTLNIYKWNYSSMNSTYVNLINQVELPQKICLNSILLQENSIIIDCYNLTTLNIYIYKDNVWTIVYSNLVQQIPKKTDLKSFTSNSINSILYAKYYDDYQILTQFQFSQNLLQNISTWIKPFINFIVSDKLQSYNTIYIWNNSQIYLLNLTQQGWNLTHNLSFVQNIIAVQIFNPQRIFYECDILLIITMQKTYKLIMCNQISYQSYIYSYTQFSLQKQPYDWIIYTQINQVFLSNQFLILQLFDLIEIYQIIDLDFNLIEGFKLSSSSTQISFDYNSNVLFIFSDTQILTYFVDYPQIQFIAEQNQSNYQFIIIGIPYQINQTDDYQCQNCTVELNVSVLPINDNQTYLIYDETSKQSQIIFESQNYHQFISQISGSLLTANFSVENSTLGNFNDITFQNVNLSFIXDLVTVLHVDCILFKGYTNVKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UKF1</accession>
    <name>A0A8S1UKF1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0410136</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UKF1"/>
    <dbReference type="OrthoDB" id="5979581at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="112"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8164643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="495" mass="57288" checksum="678000B9E770BF0C" modified="2022-10-12" version="1">MDYIDYTEIRFSFIGVRKHFFSDKQYYVSVFDDCFIMGATSNTQQPKYTIKFGFATKIRWNIKKTKQGLIIDSFSFPYKQEMKALNANHDNLIKFKEMISLKVTFDNINDLYQPLSQIGKGISAKVYSATYKLDQKIYAIKAIEKAFLKQQNESGQAAFKMEVSVLKTLAKYPENFLTLKEIYEGDQVHCLVTSYLEGSSLTQEFDRLKAADMYRFPIHQAKIIMKKLLTNLAVLHQNKIIHRDLKPDNLMFSKKNDYSTLVLVDFGLATSESMEKFLFPKCGTPGYVAPEILALKPYSKYTTKVDIFSCGCIFYKLLTGKNLFGGISFDEALKQNRMCQIEFNIPVDQQYITEQSINLLQQMLMKNPKYRISAKDALSHPFFDQNFDQNIFQQVLMFSPKRSSKTFLNVKQEEQQSDMDSYQEDKISNEKQVEESNFIQLPKSKRCSLMGDFSQLSQSPSKLLLDIHQFYQKDPLSGIRCIKVTQSPQVSQVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UKG8</accession>
    <name>A0A8S1UKG8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0410146</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UKG8"/>
    <dbReference type="OrthoDB" id="10587826at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8164663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="227" mass="26841" checksum="35F395D3D4C70791" modified="2022-10-12" version="1">MILILSLFRIVLCDIRIVHDQTRQPYIFQWDSTYIGSGVVSQYCPLGINKYLYEQSNEDYYYFLQSGYFGDKQELLYLIYMEIQFEQRLIKVDGQKEVEGIIISTIINYSESGLVLLEQLWGDNNRRSYSFPYTQYPNYQVTQISGGIYSDINPITKNKIILKQFSGKMNAAILIKDDIQYLWNGFPYYQEGKYRFVRKSFKLILYYFYLNSKGLQCILLGKSIINI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UPB7</accession>
    <name>A0A8S1UPB7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GMP phosphodiesterase delta subunit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0400325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8164306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UPB7"/>
    <dbReference type="OrthoDB" id="10248777at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042953">
        <property type="term" value="P:lipoprotein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.50.40:FF:000003">
        <property type="entry name" value="UNC119 homologue, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051519">
        <property type="entry name" value="PDE6D_unc-119_myristoyl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008015">
        <property type="entry name" value="PDED_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12951:SF1">
        <property type="entry name" value="PROTEIN UNC-119 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12951">
        <property type="entry name" value="RETINAL PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05351">
        <property type="entry name" value="GMP_PDE_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GMP phosphodiesterase delta subunit" evidence="1">
        <location>
            <begin position="25"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8164306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="183" mass="21896" checksum="CC65F7DFD5018643" modified="2022-10-12" version="1">MQQVTTEYVRSLNGITQDFLCPVNANIYNIQFLKFRIRDMDSGQTLFEVERDQDEEPIENLPPEYQDEARRIKYHFGPQFFELKTVGAQLTFSVGNQPVKNFTIIERHYFRDHLLRSYEFQFPFCIPNSTNTWEHIYTIPEIDEAMRQEMINNPFQTKSDSFYFVGDQLVMHNKAEYDYSPFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UPD5</accession>
    <name>A0A8S1UPD5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0400330</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8164316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UPD5"/>
    <dbReference type="OrthoDB" id="295417at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8164316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="428" mass="51535" checksum="3ECDC9119A7BB35B" modified="2022-10-12" version="1">MSDIIVKRREQFRTEIRKQNLEKEFSKKRFDKCNLIQDLNECNNLIDFELKYKDQIENQIQLILHNPDNQNLQKLIQLVQQLSAKYSVYIIDHNIYKTLKHNDLDLNLCLDLMCNLCVEPTVVKHIFSDHIQKERLTQYLNLISGNLFEEHRKLYWEFMLILSNSTHTPFPFLKQVFLICCEELIQCRNSTTQMQIRILPHLIQKYPQANQFYWEALSSANTEDRKPWKCLLHFLKQPNSDVFCCDQLLLAIYYLVQHSISKIYYDQIVTSELMFVLQSNLYRHEKTMVRSTKLIFMIFEENDTYFKFISDDLIKLLKITFIDGQIEILKLLEIVLRKKQHFLQLMDQYHIVDMLFDILRQDISTTMNYATLNCIALSLFQCTEIDKYQKYIQMHCSTFKDILERFVYDTNKSVQQTTEYLLRAYFEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UPZ4</accession>
    <name>A0A8S1UPZ4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="1">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0440136</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UPZ4"/>
    <dbReference type="OrthoDB" id="193499at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01926">
        <property type="entry name" value="cyclophilin_ABH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000113">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="4">
        <location>
            <begin position="11"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8166414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="176" mass="19715" checksum="EEEA4A19F09AFC50" modified="2022-10-12" version="1">MIRNPQNPIVFFDVAIAGQKVGRIRIELYADVVPKTAENFRQLCTGEYKKFDKPTGYKGCQFHRVIKDFMIQGGDFVMGDGTGCVSIYGDKFEDENFQLRHDEPGLLSMANSGPNSNGCQFFITCTKCDWLDGKHVVFGKVIDAGSMQVIRRIETTPVGPQNKPKQIIIIEECGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UTE2</accession>
    <name>A0A8S1UTE2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0460104</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8166859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UTE2"/>
    <dbReference type="OrthoDB" id="293139at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8166859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="258" mass="30995" checksum="BD9A3520CCC78DCB" modified="2022-10-12" version="1">MIPLYINYGYQQDANIVNIPKQIYLFQNDNPNMRQIQRYYQIIEKFLKALHNLQNAQNQPNFSLKYPIVIQITFNNFMVHLGKTKTDIFSKNECIFQTSNLIVPSYHTQEIPSYPKIQLPIQKKRQINFMQDQKDSKNIPKNYCKSIITFACKNQQNLCFKILKDQLKVVKFLDKISQYKKQLLNIKIFSTLLQKSDDPEEEEYRRAFRIISQIFIKKQAINYIFNSKIVQHNWHMRYRLQIYKGVKNPDNFSHIKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UYX8</accession>
    <name>A0A8S1UYX8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase</fullName>
            <ecNumber evidence="5">2.7.8.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">GlcNAc-1-P transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">N-acetylglucosamine-1-phosphate transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PPENT_87.1.T0510015</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="17">UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. Catalyzes the initial step of dolichol-linked oligosaccharide biosynthesis, transfering GlcNAc-1-P from cytosolic UDP-GlcNAc onto the carrier lipid dolichyl phosphate (P-dolichol), yielding GlcNAc-P-P-dolichol embedded in the cytoplasmic leaflet of the endoplasmic reticulum membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a di-trans,poly-cis-dolichyl phosphate + UDP-N-acetyl-alpha-D-glucosamine = an N-acetyl-alpha-D-glucosaminyl-diphospho-di-trans,poly-cis-dolichol + UMP</text>
            <dbReference type="Rhea" id="RHEA:13289"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19507"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58427"/>
            <dbReference type="EC" id="2.7.8.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:13290"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.15" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDO010000051">
        <property type="protein sequence ID" value="CAD8169373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UYX8"/>
    <dbReference type="OrthoDB" id="285536at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003975">
        <property type="term" value="F:UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06855">
        <property type="entry name" value="GT_GPT_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000715">
        <property type="entry name" value="Glycosyl_transferase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033895">
        <property type="entry name" value="GPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10571">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--DOLICHYL-PHOSPHATE N-ACETYLGLUCOSAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10571:SF0">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--DOLICHYL-PHOSPHATE N-ACETYLGLUCOSAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00953">
        <property type="entry name" value="Glycos_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="260"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="20">
        <location>
            <begin position="881"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="20">
        <location>
            <begin position="917"/>
            <end position="952"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00029567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00033238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00045078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAD8169373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1011" mass="118076" checksum="9C957E0A57386317" modified="2022-10-12" version="1">MFETRFIPCLVVFVLGYVLNGLKSALNPEQFLAFLGYMGLSIFAYVLSYWMIPKIAKLTEKADIFGYDINKKGTDAGKIKIPESLGIVPATIYLIFNIIGILFTRAYMPDLIMSHISGLLAITFIVFLGFADDVLDLAWRYKLLLPPIASLPVIVAYTGGTQIVLPQLIRPYLGQTIDLGPIYYLYMIMLSTFQSNAINIYAGVNGLEVGQSIIIALSIAIYNVIEISTQQQHLFSLMIIVPYISSALALYNYNRYPSKVFVGDVFCYWSGMTFAVAAVLGHFSKTLMLFCIPQLINFLYSLPQLFGIIPCPRHRLPRYDEKTGLLYPIKTNLTLINLMLLIKGPTSEKNLCRVLLSFQILSSFQQQPLVRKMVKSQVELSMPQTKTTSDLLFQNQRSSYQQYRKQTQQSDEISVQKLFFRTTEPSIQETPSSLYLTRQKEEPFITTTSKFQEQKYQQRIDFSNSDHKNSIIELPKFRMVASMYGRSIMSTAKINRRMAESSDSEFFMQSPGLRQMIICNQLFMEDERFLYQFMYIPDLTQPKERFDDFVKCLRNVQKINSKRLFFYLKDGTPVNSHLDIPPTHKTLIYSQNSVYKPYDNPQLDLLEQQCKSYNFLQIKKKQQYKNSNSQVEDIIRTLNDGQDNQYNSKELKTQLRNLSLRKSSLKNSLEKLIKPTQFNVDNNLKLQKTVINTQIDKTETQEQNKMELFDDMLQLYEQELKKIDFNQNQPDDINQIQFDNENDEMRLKIKQAACLLEPLLNKFLNFTQKKEEIIEEQFQLNNMEQKRQSKSQQNFVHKNIKRRISHKFSLQQLLKINRELFIQNIPKLLSSSNFSRYELHNTYVLYCALQQITSQRYKYYNINDGVDYQTYRRGIFQIFFQNEFLAQQIFNRIDYNYSGFLNWEEFLRLMMSIKAKTVVEKIDLFISISDSDGNGRLSHDEIFKLAKLCLSHYVQDKGEFLDILCEYYTRLIFQIVNKEVTEEIPFEEIKKAILENKEDSDLLLMFCGADV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V0G0</accession>
    <name>A0A8S1V0G0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0500242</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8169259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V0G0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8169259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="92" mass="11443" checksum="6C91D787A2F1C0A3" modified="2022-10-12" version="1">MDFYLRLQFQHLFHKDLLLNIEQIINCLSYVRNTFIEKEIEIPINSNHLQYFNISYLIFRNLLPYQNDFQILNYQRRLNLIFLIIIITQDYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V2I1</accession>
    <name>A0A8S1V2I1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0500086</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8168976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V2I1"/>
    <dbReference type="OrthoDB" id="285193at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8168976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="82" mass="9506" checksum="BBD771B4B279D901" modified="2022-10-12" version="1">MEQKHLMRYYVEEQQALHRAAAVYGSGLAMQLNLERNILGAPGQSSYIHLEIAMNKLNDIDELDFMGNKKFEQEYVKGFQQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V454</accession>
    <name>A0A8S1V454_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0560113</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000056">
        <property type="protein sequence ID" value="CAD8172150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V454"/>
    <dbReference type="OrthoDB" id="311088at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="187"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="221"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8172150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="271" mass="32150" checksum="296BCFCB2FD8514D" modified="2022-10-12" version="1">MLRTSQCKNRSPIKKTKKKNTKKLKGQIISLTKNIWNGQIKLNKKNSQFRSISYIQTCFYKSRGKSYFMKLIDYISCKRIIFYFIYFQDKYWLRNSFIKVLDNQRQFKIEKKIKIQFWVFAINTNSIIYSNDKQYDEAIKILDKALSFNTKHQLSLYSKADSLKMLGQYNEAIIWADKALQINPKDCFSLRTKGDSLRLLKDYKEALKVIDQSLSINPNHLSSLQSKGHCLQNQNNYQEALLFYEKALKIDSNHQWTKNRKDECLKALNTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V4R3</accession>
    <name>A0A8S1V4R3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0530274</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V4R3"/>
    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8170842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="324" mass="38027" checksum="557E8264A2DBDFE8" modified="2022-10-12" version="1">MIQRLMSYGRMSNVSQSVLSKSLNKTSRSYFSQAPQQGSSNTELAKIFAAFFGIVGLGTGGYVAYQNSQQQKKLEAKTKQTHISNETIDNLRQKSIENEEQKKEEIITMFSDVNDRMLRYRGDPTSEKMLADYTIYGDLKWFEVDPEEWEKPEEEMIMKDENEINKHPIKRALINERLTHAFEAEKILQSIFVNVNDVLSEIEKTGTYTPPGIFFRFYGYVYFQIRRKPINVENLVKAQKYISLFDLFFQYQTPVLYYHNQYLEIPLKPAIFDNIDWLPNAYINSKQQAKIYITGKPKHVFSMRIKQGTVDESTQRFAWTNHYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V7Y0</accession>
    <name>A0A8S1V7Y0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0560071</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000056">
        <property type="protein sequence ID" value="CAD8172072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V7Y0"/>
    <dbReference type="OrthoDB" id="307746at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="109"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8172072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="198" mass="23237" checksum="78415671AEEB2A01" modified="2022-10-12" version="1">MINFVDTYEDQKLEQFEEELELFGFLSSPNTLILSDQGSSSCVDQDSNLSTPQQQEIQKIKLPIQRRRPPKRIQYTYHKKKKRPAPKPIIHIKENGLKVCLVMEKPKSNAHPFSNIEDKRILELVLKIGPKFYKISKSFPGKSVSMVKNRYYKYLRYRWDEVMGSEYRHMNSLPEDQVIESQNGFRSSDISDELDSEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V914</accession>
    <name>A0A8S1V914_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0580202</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000058">
        <property type="protein sequence ID" value="CAD8173075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V914"/>
    <dbReference type="OrthoDB" id="10264376at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051858">
        <property type="entry name" value="WD_repeat_GAD-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16017">
        <property type="entry name" value="GASTRULATION DEFECTIVE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16017:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="19"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="120"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="223"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="406"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="406"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8173075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="520" mass="59018" checksum="9475AE2D3171EAB2" modified="2022-10-12" version="1">MELQKERLATFPITHQLLIKGHTKQATAIGIDNAGSRLVTGAYDCVVRFWDFYGMDKNTHSFRLVTPYEGNQVNQISWAKDGKNVLIICADAQACVLDREGKRTMDTHRGDMYIRDILKTIGHTAAINSGCFHPDFSDVFVTCSDDGTVRIWDMTKKLYGVAQCLTHKDAVKCVDKTGHKVLVQCVTIRGRLLLCACSDNTIKAYEVNQNCREIKKRPLFIIQDAHKGFVTKIKILADQKRFITQSVDNTIKLWETTNTNHHIYLQYCNINRFPPTRFDLNFNESILVTAHHTFKDGYHIVGPTELLFLNVNDLSINCRVQLFDQAITEVLWHPTLNQIFLGSDDGHVRALFNPKLSQKGALLATNRAVKFVHAENMELERHVLIPENLYIFKVSETVHGGMKIVEEKKSVKDQPRKPEPPQKGPGKGGKRSGPATLTQNLMSTLNDRPEKEDVVEELTKLDAITKANPQYVAQAYKYTQPDQIFDYSTNQQQEHTFLSSIKKICPHCGMKICACKRKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V990</accession>
    <name>A0A8S1V990_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="13">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPENT_87.1.T0580237</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19 13">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="13"/>
    <dbReference type="EMBL" id="CAJJDO010000058">
        <property type="protein sequence ID" value="CAD8173145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000118">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000084">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF150">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 12 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="284"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="328"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="883"/>
            <end position="903"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="915"/>
            <end position="935"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="986"/>
            <end position="1006"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1018"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1049"/>
            <end position="1070"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1090"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="15">
        <location>
            <begin position="29"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="16">
        <location>
            <begin position="851"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1166"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1301"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1516"/>
            <end position="1539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1166"/>
            <end position="1180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1181"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1311"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1516"/>
            <end position="1535"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="10">
        <location>
            <position position="399"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="399"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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        <location>
            <position position="399"/>
        </location>
        <ligand>
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        <location>
            <position position="400"/>
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        </ligand>
    </feature>
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            <position position="533"/>
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        <location>
            <position position="629"/>
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        <location>
            <position position="709"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <sequence length="1764" mass="204873" checksum="70380A699E92E2C8" modified="2022-10-12" version="1">MFWSKRPDVYNQPRQFEGNFKSERTLLELEQFPSNFIKTSRYNIVSFLPKSLLLQFTRYANIYFLCIAIIQCIPILSTLNPFSAIAPLVFVLGLSMGREGWEDYGRHVSDNEVNATECVIMKSRVMTISTWAELAVGDYILVKKDESFPADLIVLGSSIESGACYIETSSLDGEKNLKPKSAILETQQLYSDKNNYTDQIIRVDAQVPTQNLYELDASIYLSTGNGQQKKFQLTAKQLLLRGAYLRNTEWVIGLVVYTGQDTKIMRNADAARIKSSEIERIMNILILGILVVQISLSIITASFSSAWLHKYGSDSWYLGYDDDFSPNLLSFYSFFSYILLYNTMIPISLIVSLEFVKVFQAYFIEQDEEMYVQQRDKYVKVQTTTINEELGQIEYIFSDKTGTLTCNQMEFKYCIIGNTLYGQEQQHTNIPIQNTDLKRQQTAKVHPATEVFQHSVFNFQDLELSAILKGEGSSGELPVNLIIQSLDGKQQIKLQKQRNLIEEYFFLLSSAHECIIQYDKNQNASYQGPSPDEITLVDAAARMGFQFTGASASEQKFTILGKDKKVKLLKSFEFDSTRKRMSVIIDDNGVIKLFIKGADNIIKGRLAPNQIFLNQIINYLDDFSKIGLRCLLMAIRILSNDEYREFDNAYNNLPDNETRAEELEKLTSNLEQNLTLVGASAVEDKLQSLVPETIADLLRANIKVWMLTGDKLETAENIAKSCRLIQGDFTVMRLSESTVEACKQKIGDIQDTYEQCIKMNRKKSFVVEGQSLQFVIDNEDLAQAFVSMAKDCESVVCCRVTPKQKADVVRLIKDRLNKITLAIGDGANDVNMIQAAHIGVGLYGNEGMRAVQSSDFALGEFRCLWRLLLVHGHWNYIRIAEMVLYFFYKNMIFTVPQFFFSYFCAFSGQSFFDDWYITFYNLIFTALPLIVRGTFDQDINYRQYVQFDERQEVASVQQKFEEYLKTKFPTLYYVGQNKSIFTIPNYMFWAFTGLVHGMIIFFFILWIQDFEIVEDNGFPGGLAAFSLTAYSSIILIADVKIAIHTKYWTWFNFVCITFLSVLLYIIYVIISQFWSGTLMEFTPFTMVGTPHFWLSIILIGGIIGGLEAILAQIYREFFTDSATELLIKANDFGKNQLKLIELKEKNQRQFQDSFWEPIYKQQQQQDQEIEQQQNEFQESQTLRQQGPLQTSNRQLTGPQLYNLNKHTSYVFAAIVHNVILQESYDKCNEFDDKKKPDLDETEWKKLKKTLWEEKIEKQNKQTLKQQISFIVSAGADKNIYLWDIHKMKLQDNSQVPIKPEQLPQQQNTQIQSNSDNQGNENQNNQNFSKDELDLEHVIIPYQQNFYEFVTCLLSVELNQNQSYLIVGYENNQIIRYELSGEYWMKGVNSLRYNFNFQNMSQMITKLQHHNDAVRSLVYYKDQLCSCGDDMQLLIQTIDGKVLKRFFFDSIIDNLYIWNNHLIFPHFTVSLQPFSYEDEETRFVTKKNVQIEMENENQNQNQNENQNQANQLEAGAQNQNQNQNQENQQEAGAQAQPKEKQKITQIVEKQTYTYGLQQCLMIMGENQQNNYDIKLNDLMFYRSLDDAYNQMDKITCFELSDNKNILYSTESGKLIMIQEPLRNHSKGETITDNYKDQENKNQKYKYQILFCNTKQLFQFDIEILMSFIKYESKLYIFCKKKQNKEEVGLIIGQEITDSKDYQYKIVEKPLKIEYLQIYQKREENQPDPNPIIKNYEVFLQNPILVKEGDTKIILFCGDQLRYYKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VJW5</accession>
    <name>A0A8S1VJW5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0660049</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000066">
        <property type="protein sequence ID" value="CAD8176883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VJW5"/>
    <dbReference type="OrthoDB" id="6262491at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="246"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="341"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="44"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="162"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8176883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="513" mass="60001" checksum="876C02DA9AA81F1D" modified="2022-10-12" version="1">MLQSFASNVNCPNHNQPITYIDTKKDTPINNRAKCLNCIPLNDAVQLKIALEKFKKLKEDEKQQIRNYRKNKIYDFLEFKKVIHQLSESISQSLNEISKQINERIEKIVSVIQQAQSVQWQFDINEYLTISGLQNIGLQLSQQGNEFSLIKAGYSVVQMKKEQQLQTTLSNYQNNIKSIQSNMEILKESLTTNLIINEEIVGKAPQKEKCLATAFNKDNSIMISTSEELIKVWAFDNGKLELFQTLKGHDKEVTCLYFSRKNNWFISGSRDKTLRIWKEFSNSEWGCIQILEGNSFCINCVIMNQYEDQIISSRDDNKIKVWMQESLLTSQQPQWKCYQTLQDHKKDVYSLSLSSSGTMLVSGSYDKTIIIYDMDDQQQWRLKQVISNDYENWVHSVCVINDKLIATKLYNGVVILYQYMESVESFEQIQELELSKSKMSYFDFFHMFYNQQLQSIFCRHLDCIYILFQQTTGRYGVQQKITDNYRGGSLTDNGEYIALWNNSTKQLEIRQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VPI5</accession>
    <name>A0A8S1VPI5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0670266</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000067">
        <property type="protein sequence ID" value="CAD8177552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VPI5"/>
    <dbReference type="OrthoDB" id="283324at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF8">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0810">Translation regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8177552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="204" mass="24177" checksum="75017DFF3AE5B767" modified="2022-10-12" version="1">MCDQFHQLNDKWTISEKYKAQNSKDDYKKTIVDICSIKTVEEFAFIMKQTIYSKLTDIFSEPQKQKQFKNFQNNAIETQIDAIQFFKNGINPCWEDPENEKGGDIQFEIPITSIDIYNELYTNIIYEIIGQNKEELRHVNGVRILDKINADNGIRIELWLDIDPIDKNEQVQNNIKKIDDWILQLAEQYNIKQLKLKTQSHKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VPQ4</accession>
    <name>A0A8S1VPQ4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0710025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000071">
        <property type="protein sequence ID" value="CAD8178997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VPQ4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8178997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="207" mass="24771" checksum="E04D504765183327" modified="2022-10-12" version="1">MIFLLCIIDKVITFHINKHANFVDKVRTALIIIKNSEISIFHIYLMYSINYISIFQQIIISIQRNHFLMKIDNSQLIKVNQQIQNIVLKFHVIRLHVSLKLEYLKEEYQMKNIYLLISDILIFYKIQITKIVLWIFSQGCNENPSSRFIRISINSTLFGIEFIFQKFYLTIYIGKNLEQILKNENDCIQKRLLNIIKENGMYLNMQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VTD2</accession>
    <name>A0A8S1VTD2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GMP phosphodiesterase delta subunit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0680077</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000068">
        <property type="protein sequence ID" value="CAD8177776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VTD2"/>
    <dbReference type="OrthoDB" id="10248777at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042953">
        <property type="term" value="P:lipoprotein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.50.40:FF:000003">
        <property type="entry name" value="UNC119 homologue, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051519">
        <property type="entry name" value="PDE6D_unc-119_myristoyl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008015">
        <property type="entry name" value="PDED_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12951:SF1">
        <property type="entry name" value="PROTEIN UNC-119 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12951">
        <property type="entry name" value="RETINAL PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05351">
        <property type="entry name" value="GMP_PDE_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GMP phosphodiesterase delta subunit" evidence="1">
        <location>
            <begin position="25"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8177776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="183" mass="21796" checksum="43159C6CBC73E6E1" modified="2022-10-12" version="1">MQQVTPAYVRNLNGITQDFLCPVNANTYNIQFLKFRIRDMDSGQTLFEVERDQDEESIENLPPEYQDEARRIKYHFGPQFFELKTVGAQLTFSVGNKPVKNFTIIERHYFRDHLLRSYEFQFPFCIPNSTNTWEHIYTIPEIDEAMRQEMIANPFQTKSDSFYFVGDQLVMHNKAEYDYSPFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VVP7</accession>
    <name>A0A8S1VVP7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0760132</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000076">
        <property type="protein sequence ID" value="CAD8181420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VVP7"/>
    <dbReference type="OrthoDB" id="407343at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8181420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="329" mass="38689" checksum="33CFBFC75304B97E" modified="2022-10-12" version="1">MQRKESKKATIPKMVARVLQQNEASDKLTNFLFIKQGQDRIKRTILAYLIGDFTNLILVSGQWYVGFQQTLKEWLEDLDNRFIKAHLHILSFKNSDFLQQSFWVDSTKTKKLFRWDRTIISEVLNGFNGKCITIAFKYNRKYRCEYKFDVLPQNSKRVIWIAREQTKHNFESVSQVMNIQPILTGDCVKIAINFYNKLGFIDPDTIEFEDPQIEQSKERICSIQKQFFDWVGIEYAKQRPSLRDYQIQPHLRLINCRCAGVDTVAYQFFYEACEIGSFKNDLLGIPIEVVQQGQEVVTELKKVGLVSDRECKLQLRKQDQLIFYQTTGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VZN8</accession>
    <name>A0A8S1VZN8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0780115</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VZN8"/>
    <dbReference type="OrthoDB" id="299631at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="221"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="13"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8182281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="438" mass="50499" checksum="96973CA1372563D6" modified="2022-10-12" version="1">MNAMKKMSESRHFSSQQQESSPLMNQSTNQSTKKRNKNKENINLFNEVNSLLQYYQNSNILKKKTSLPTSMVSPKKMMDDIFLKQVLQTRKTNSRNKVSVSQNVGQKVLSLSPIEQKQKSFTKGNQFRCAYHANKKAKFFEYNRSIGAIFYCSECSVGKLIKGNILFPVENYQVKQVSNKKRENQSVSRANFWSVDENSLLSNSPFLLESTDTFTSITDDTEDKAKELKDFQKSLSSLQQECQSIIDNAKGNTYQTQFNTLNTELQQLLYMINIQSMKLLQQLQSQNHLQQVYEVQNELNSILSDIQSNESNILNNMSIKPFKQIMNKYKEKLKGHTQTINNCSSSQQIFELDDRLQKLNKVKKLLKTNFKIETDEQELLSTKRKLSSNVYISFENKEMFLAAAAANKANSIHAKDEPDLVQIDDKCHLLESPNFIQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W274</accession>
    <name>A0A8S1W274_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0830077</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000083">
        <property type="protein sequence ID" value="CAD8184468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W274"/>
    <dbReference type="OrthoDB" id="296244at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8184468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="400" mass="46895" checksum="F4B1ECC2EE81482A" modified="2022-10-12" version="1">MKNKDQLNLCFRLVQYCLPNLRLRNQFIIIVAVFVMLIVAIFALWNLAHQSILNNLFLAESDILHDTQSKIRISFMLNNYKSYFYQIFALNGNALISFHRLYLRTKKEIMKSSLEIDERFQMEYEGMIQIPDPLRILNEYGSNISNSFMCYSNTSAYNNPLTEEQRIGIKLQEQIQAYGQILYQGSLIMQSNIYSYIDLQKINNLYPCVNRGKGIYTYAPEKRDWYKTLKQTYFNTTKYDSYTFKFTAPYLLFTYQTIGLSMTLPIVDENVKFVGGVSSTFLGSKLVQYLSNNETGFQLIYLVSEDGTLIMHPFTVKVEQLPLYIFNESYTGFNLTDWDEIKKSDGLSSCQTFNLRTTLKCRYNSVTQQEMIVSIENLIEYKMYLIMQQIIEDNKQVIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W6F0</accession>
    <name>A0A8S1W6F0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">legumain</fullName>
            <ecNumber evidence="3">3.4.22.34</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T0840098</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of proteins and small molecule substrates at -Asn-|-Xaa- bonds.</text>
            <dbReference type="EC" id="3.4.22.34"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.34" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8184971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1716625at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006624">
        <property type="term" value="P:vacuolar protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1460:FF:000006">
        <property type="entry name" value="Legumain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048501">
        <property type="entry name" value="Legum_prodom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001096">
        <property type="entry name" value="Peptidase_C13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12000">
        <property type="entry name" value="HEMOGLOBINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12000:SF42">
        <property type="entry name" value="LEGUMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20985">
        <property type="entry name" value="Legum_prodom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01650">
        <property type="entry name" value="Peptidase_C13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035854272" description="legumain" evidence="10">
        <location>
            <begin position="18"/>
            <end position="1609"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="11">
        <location>
            <begin position="726"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="13">
        <location>
            <begin position="1072"/>
            <end position="1188"/>
        </location>
    </feature>
    <feature type="domain" description="FERM" evidence="12">
        <location>
            <begin position="1270"/>
            <end position="1605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="424"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="497"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1448"/>
            <end position="1482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="429"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="514"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8184971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1609" mass="189836" checksum="E47BA51E0CA26664" modified="2022-10-12" version="1">MKYILLLLTIGLIQVNAVNWALLVSGSNAFYNYRHQADVCHSYKTLIKNGYSPENVIVFAYDDIAQNRQNIYKGAIYNQPNKDGFSDNVYDGCVIDYSKTDVNPTNFLNVLKGNYDHLPDGHKFINSTREDNIFIYFSDHGSPGLIAFPTSYLYEDELMETFQYMYENNKYNKLVFYLETCESGSMFVNLPKDHRIYALSAANPYESSWGTYCPPDDIVNGKSLGTCLGDEFSVTFLENVDLADFSQSLQEHFEFIRDHTLKSNVMQWGDVSFTSDTIKEFFWGHKFQNNRKMCPKDAYFMNDENVSKWDSRDNKLLFYQNRFNQTGDLEDFIELEKEIKSRAYFDTIFGELQQTLKLTGDYHFALNQNCLKSVIGVFEEKCTKLTDYGLKYVKLFGEMCDSTNLLQIQLNMIRIQTLQRINQQGQSKNSNQEQQHLNSNQLNQQQTQTGQVVGIVQNNIKSFEQEISKEEVDERDIMQSRQFSIFRKSNQQKQPAIVRYKSQIVNSDDESDDDKNKIKNDKKDEDEDDEEVEVKVWTAKKATTDQPKQDQIQQIPIKKAEDIDNLKPLDFEEVLKQSQINFSLNRQNFQRDKLVNKSTFGAVLITHVDSEDEQQNKEELADEVFQEIGTIPNQESELTAENRIMMSVMQTKFTQSIQKQLKLQQSLNNSSKSNQQDDIFKSKIIFINQTPVIVREDDLYISKIRKIQNYFRFVKPYKRIKKQHRYRVNVINELIQTEKKYVSDLNVIKEHIQAPLLKLLDNQDHVKFMFNLDSIYYFNFEFLKILQNKEKDFKEKPYAKIMDEITVLLPGFKFYYDYCKEFDASKKMRDNYFSTNQIYKKFFIDLQQKKINQLKNQDVESYLIKPVQRLPKYILLYKDLLKHTNKDHPDYQNIEKCLIIFEEINDKNNNEMKIYLEQLKLIELQNQFSQYVKIAEPNRVYCFEEFCTIYTDKKENNIILYAFNNLLLFAQKKQNGQQIYSFHINISYQSYVKDKENTNYFEHFFEVVNKTESIIIINQDKESKIQLMNKIQDIIQQLKQKQMDMEIIRKTKSSIIEAGDYQKEQKQQEFDYEIKVIIIGTEIRNTKSNPYTVYVVQIYISEYQTKIFVRYSQIVSLQSIVNKFDSTLKVPVFTTLNWFHSIDSKVIEERKLLIEKFLSSVLNSTKCQNSIEYQKQILELLSLNQDFFQIPKKKNMASQIKTGEEDMMKMILQSQFSNGIKPNLLQSMIMNRQSTNILQAAKAQAQRSSLMNMEQDKSVVIGQNATKSPYFIDVSLMDGRTIAVGFQKQTLTLFIKNEVAKVVGLKQWLDFRLFIVDQNKDQRVIDDDETMSSILDAHTNQNKGLMNAFKKLFTYEQKFQFIFRKYFYLSWKQEEADLKQDEQRLRYIVYDLIWEIRNEKFQFNFNEYCLISALFYYSTNTSQDQLSTLLTKTIPKIILKSKKEEVWLKEVVNNINNLQQQLKQVQKENQSQINKNKKLNTSISGLAQLMIMNFFKNNLLFGMSSFFVECDKGTIQLIQKHFNLKIKSNIFIGLNYNGFHILKPENKALIFTCHYQKLSEMKACTTEFCCTIDKAKLTFKTQFPFEIKSLIQEYLQLQEFTKQLTYGIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WAC9</accession>
    <name>A0A8S1WAC9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0840037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8184849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WAC9"/>
    <dbReference type="OrthoDB" id="295616at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8184849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="496" mass="58911" checksum="6EE79A3E7F9A90E6" modified="2022-10-12" version="1">MNKYNTRFKDPNLEKQYIIHKVKVIQYPILTYLTFGGTILLLANSIANLINSDYKNALIKGAVMIYIMLQYIVLRRNESIRMNYCNLALMMTNFASLAFEISIYPQYDYYDGYLLGSNQMLVHTICMFACNLELGIFSNIVLTIVRIILVVKNSDNLTVQQYFYSIIMSLSFMVIQYQIEKQYRQSFYLQFKDNNWEILIPLLLKKPFFIFTFNKETNMYQTIMSAQKEFFNSEKPLSTFLFKSKVKQQTLEEYLIKKTVIHANRKQSSCLFNHQLVVEYQMKKLQTSILCCRFEQLIYAIIIDSEDPLLNQTNIKLHQSLQHFKNILNAQLMQINKVLGLYLQDTFSPLLRDLRISLFEVYYKQLSNQKLQLVKMKKLLLKCIKIFETPYTRIKIDCGEDIRFATIKSTLIIFIFEIFKQIDKNQQVYIFVNQKNTTTMMIYGLKQLPKSTQFEKCFQTLIDKSNFTSQYFSFEFLKSPSSHFHFENHNKYQQLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WAY2</accession>
    <name>A0A8S1WAY2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WD repeat-containing protein 65</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0840137</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8185049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10251741at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052993">
        <property type="entry name" value="CFA-57"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32215">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32215:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="530"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1363"/>
            <end position="1383"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="761"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="1202"/>
            <end position="1250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8185049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1383" mass="160845" checksum="992110D5B9B1F1B2" modified="2022-10-12" version="1">MDDKKRRNEDRRLKLDVIQSYGSLCSAKFKDSISIIEDEQRRLLYPVGKFIAQKFIDKPDTQFIRLSDNLEIVSCMTVAPNKRTLAVAEKMKNETVPQLVIYNLKAHPSKMEAEKKPYKYLDTKSSTFISMAFSHTDSRFLLCLTGPPDYLLIFVDLIRMKHLALSAIGQELTRVSISPKDNHMVAVSGKNYFKILRVQENSFISLTDQIKRLAPIQTFTDHAWFEENKVVLANDKAEIFIIHDNDVRQYINNAFNDIRGPGISSLIAFSKGFLIGSDSGHFALWIRGDDHDKQKTEDGIEVIMDLQFLKKWRCERKSQVVSMDITKQEDLVAVAFSNNDICTIEMGQVLPNSTEVLESYKNNEKFLKREVKFDFLFNGFHNAPITHLDVCLQRPLIVTASKYDSTIRIWNYAYPRCELARKFYLGDDGNQQGLGTKENQSTTQDISPLCSVGFHPTGYYLAAGFQDKLRIFHVLHSELRAYKEMAVKCCTQVKFSIGGHMLAVAYPRAKSNHYYINIYDAYTMEFMHTLKGHSNQITDLIWGIKDTFLASCGLDGSIFEWNPNDWSRKDYVYNTNKYTSLIYDPPIGMLIAAGTESIKDKTEQDKIDTKYIVREQRIVQNGISTGKDHHFHDLGQQKLTQLAYIQSLYNQFGIIGGTENGQIKIYSYIFNQMAFETMPVHQGEITRIKCSPDGRYVFSTGTDGVLFIYQVSEISNDGQIYASKTGAEAKEQMDDIDKINPKAGVVDENLADVLLVSRNEIETYIAEMKKLHNDLQDLDQKTDFKVQEEKRKVEKEKLEIEKQMNNEIAAWAARYDQLKAQKSAAEKESANLFKNVENNHLKAVEELENLYERKLAFENEKYLQLEQDLMEERRRNQAQAKEFESKHTSYIEELKEKFYKNFQITAKALENNQNQADQLKATYEEILAQQEEDHEFEIKDLNDRQKKEIDRLKCLLAEKDSKFKQEQKLVQELRLENEKLDKRLQEKEQAYQLLEKNLKEAETKIDVQCKDLEDKDDQLKKKDKKISEYKDKINVLQKSKHVLSFRTTEMKKSLEPKEAQIEKLKEELFRLESEFAKQLKINSELNSKINEETSKTNALTHDLNLQIQTTKKKENIIQNITRDIHNCVHYIDDKEWKKEMTKLYQRYVLQQEIKQSSNDPRSIEEMNRHINHLEKSAIQINSSTEKMMVRREKEIYKRTSENQQLIHELNEIRKQCKDYETEKSNLKIENDKYKKENEKFKQEIKVLQTKLGKNIGEQNVEMMNEDVMQQSQQPILPSALNINKQGSAQARLQSLPQKSNKVGKILRGPNFDKQKLQPFEFQKNVELQNQLQIAINQLQANDAMIKNFKKILKEKGIEDPYADAEQEQSQFGRPLSSIFGSRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WB13</accession>
    <name>A0A8S1WB13_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0860140</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000086">
        <property type="protein sequence ID" value="CAD8186063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WB13"/>
    <dbReference type="OrthoDB" id="297052at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8186063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="570" mass="68216" checksum="6791961489AA0408" modified="2022-10-12" version="1">MKDLRNLLIKDNEDLLSDKREQLYQQQAISRRTAATRESGRAVGSRSYQRFFKIDSPTGNLGNLFNNHKFSLQQDAFNYQVEQQEKFVVKQYWRSLLRWQGFDDYLFLRDPINLRHIKGREILTKNDVVEELQAYNIHVQVVYQADNGMQEYKDQHRRQQMQQLQKLYQPGEANYQKFNPEKYKQSFFGGKDTGKLQQMVFENVKLKELIDKIQQILQLINEKIVSEEINQISPLGERMIRLLWKIGCLNLFLLGYFIHNMILEIYGKEKLAYQNWKKILRICNMQGNHCHRYKLIAYKHISKICIKLQLYDKSLIYLKKMLKLSWIINDTNYEIFTYDKISLCYYYKQDMNKAIYYHEKFAQGEYEEPGKGMRNVGEAAYLLDSKMKEGFIDQNSYSEDEYDLDVLLQNGNLNKWELENKRKDLKVMAKKMPTEYKKGHSFGKIHRPTNNIIDFKHAMLILHKFQNKQQIPLGPIYSKDIPDQIQSTKRWKKKDPLQSCQYLFNQKSTNRVAQNFLLQEEIKEQPISDAICFKPSENVYYIKKMIRVFQYDLKYFLQKQSLFKNSTDCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WB53</accession>
    <name>A0A8S1WB53_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Calcium-dependent protein kinase 1</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="ORF">PPENT_87.1.T0850214</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cell membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Parasitophorous vacuole membrane</location>
            <topology evidence="24">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="30">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WB53"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020002">
        <property type="term" value="C:host cell plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020005">
        <property type="term" value="C:symbiont-containing vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000398">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000299">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000383">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000828">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 26 27" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17" id="KW-0969">Cilium</keyword>
    <keyword evidence="15" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1032">Host cell membrane</keyword>
    <keyword evidence="16" id="KW-1043">Host membrane</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0519">Myristate</keyword>
    <keyword evidence="12 26 27" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0564">Palmitate</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 27" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="28">
        <location>
            <begin position="26"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="330"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="368"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="401"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="437"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00060437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00068067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="EMBL" id="CAD8185675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="473" mass="53911" checksum="2ADA15D3EF40C0DC" modified="2022-10-12" version="1">MDQDVFLRKQWFIMTHEGKIEQFYDINLKKVIGSGTYGSVVKATLKGTKNQRAVKVIPKSKVKNPDRFKKEIDILRQLDHPNIIKLYETFEDQRNVYLVMELCEGGELFDRIMDKGYFSEAEAHEIFLQIMQALNYCHTNGICHRDLKPENFLFLTKADDSPIKVIDFGLSTLFEDPISQKASGSQKVTMKTKAGTPYYISPEVLKGSYDESCDIWSAGVILYILLSGVPPFYGDSDPEILDAVQKGEYTFEIPELKQVSDSAKDLISHMITTPEKRYKASQVIQHKWMKDGSKNTKQLKLNFGQLKNFNGSNKLKKVALTFIASQLNEQEIADLGKLFKQLDKNGDGVLTIDELREGLIGMSDVQAKELGNIIKSIDTDGNGTINYTEFLAATMEKSLYMKEEKLYQAFKMLDLDGSGKIDKHELQTVLGKSEKIIDEKYWDDMIKEADKNGDGEIDYNEFIEMMDKFSLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WG50</accession>
    <name>A0A8S1WG50_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0890167</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000089">
        <property type="protein sequence ID" value="CAD8187617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WG50"/>
    <dbReference type="OrthoDB" id="329563at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007091">
        <property type="term" value="P:metaphase/anaphase transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558">
        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558:SF13">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 27 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="119"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="428"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="496"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="530"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="598"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8187617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="655" mass="76235" checksum="F5C94379C42519F7" modified="2022-10-12" version="1">MIEEEGQQLKSQLEQQLVEAILDSLQNHMDQNATFLAERLVYERDTEEFRSLLAECYLQENQPYKACHILKDCKSEYNRYQYAMSLFRNKKYKEAEVALVGTQFSNQFSIQTPNVPNGGFGYLLLGQILEQLHRIDEAKHQYYRALDQNPTLWMAFERLSKIGESVTINKVFNDQKQKQYEINRQQSCNIYKIIQNLLKNKSPNLKSGQKEEDIKEEAQINDTQISSKRQSQTIKPFSTTGVQQSIVHMDDNSNSQMKFIQHQQKKISAKMVPTPGVGYQNYQSLLSQSFQFSQNQNKNFNPKKSDIQKVNTAAIQQSPSLINLPQLLKLFAHPYNLWTNYQIEAISNFQKLPPQHYRSGWVLEKVARSFMDQVKYADAERVWKEMKQIEPNRLEGMDYYSSCLWHLKKQSELTYLAHSCLQISMQAPETWIAIGNCFSLSKEIDNSIKFFGRAIQLRKDYSYAYTLSGHEFSQNENFQQAKKSYDMATSLDQRQYNAWWGQGNMYYKTDKYDDAIRCFTQALKINSNNPVLPTFLAMSYAAKGEHNEALKYFEQSEKLDPQNGLNKYQKANSLIKLDDYDKALSELQRLRELIPKEAAIYILMGRIYKKLGKIQEAQNCFSTAISLDQKDSAKIKGLMESLNNPNSEFNDDFDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WGS7</accession>
    <name>A0A8S1WGS7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0890036</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000089">
        <property type="protein sequence ID" value="CAD8187359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WGS7"/>
    <dbReference type="OrthoDB" id="28293at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8187359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="619" mass="71443" checksum="285A16D429CCA1D1" modified="2022-10-12" version="1">MIIFIALAQITYQKIIYNFDANLDDPDDWSNDHYTHDFETCDDIQFFGSISEESVISRLFLNLESHSAIQVEAYFLMYVFINFKSYDPYNNVQFLLDDTSIEYEETNIAYSNYTCGPIFLSTISIIHQHNRRSGWIKIFQPKGGIIRLQLSILNCQEECIACICNYPISCVKWNLHQLSFNQKLLAFPDGWTLTPNYLEIATSFCGGCEYLKFQEISYSTELPPHQHILIRFYKGSSSSIQLDYMQDKLIFLENEYFVEILIKNYFNSILNLNIKTVLSTDQSWIRDFEIYYTLPEITFLSLNEGCQDQIGDRCLNCRDGWIEDEYQSNCIPICGDTIIQGYEECDDGNLISNDGCFHCKYQCFDSCKICIFGICKLCLNGFVLNENNNNCEPQCGDGIVIPYSTEQCDIKSEKDLSGCKECRYISIPYCQQSYFSTCLECYQGFSILDNVCYPYCGDKRALEQIEQLNDGNLTIYVNCIFCDQRFCRLKCKDGYEYINYSCLPICGDQIVTIEEECDDGNTIMFDGCFDCNYSCPENCHDCYQGICLECKSQYLIQESNQCKLQVICGDGLVQQQEECDDGNNQAADGCMNCLIEQNWILQANACMQKLPIWQLIISI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WH29</accession>
    <name>A0A8S1WH29_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0920027</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000092">
        <property type="protein sequence ID" value="CAD8188663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WH29"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8188663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="150" mass="17890" checksum="8030E4F86A7D72E3" modified="2022-10-12" version="1">MQLQKTVQNNLVQFHKDNNSNIHAYQHFLDIDIFEILRLNKNIQKFQNIWNNRTIETNKNCCKARIVPLIKFFPDIPNKSQLIPIKILSSLFKFLELRFLPKLQMNKQDSYHILSTLLNIINLLNKINGFKKEDKMEGVFIGLRRSFLIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WI61</accession>
    <name>A0A8S1WI61_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0920037</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000092">
        <property type="protein sequence ID" value="CAD8188681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WI61"/>
    <dbReference type="OrthoDB" id="10255013at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15848">
        <property type="entry name" value="SNARE_syntaxin1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF307">
        <property type="entry name" value="PROTEIN SSO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="4">
        <location>
            <begin position="197"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="80"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="228"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8188681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="297" mass="34404" checksum="81920DE1E1CEAA55" modified="2022-10-12" version="1">MNDLYYQLKETAQQNCITIELIPSNRQSIAEMMPDFQKKSSMIRMKLQLIRNNNEEISRLTEVIQTAASTQKEKESMKKVEQLQVQINKNTTEIKKLFEEIDKLVKEGDPDEPETRAMIYNQKAIQQEVASILQSGQNTIFNYNKTIQKKMKRQLEILEPNLTEKQKTDIISDPQGLEKMVLKETLGQPSLQLVYRVQDIQDKFQDIQKLEKSTQYCFQMLSEIAFLVNQQGEQIESIEQNLNKAKNYIEKGEKKLAKEQEIHKKSRKKMCCIILIGLIAIGVITTPIVLKFVKSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WKQ7</accession>
    <name>A0A8S1WKQ7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CHORD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0950148</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000095">
        <property type="protein sequence ID" value="CAD8190133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WKQ7"/>
    <dbReference type="OrthoDB" id="10261079at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007051">
        <property type="entry name" value="CHORD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039790">
        <property type="entry name" value="CHRD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46983:SF3">
        <property type="entry name" value="CHPADIPLOID STATE MAINTENANCE PROTEIN CHPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46983">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04968">
        <property type="entry name" value="CHORD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51401">
        <property type="entry name" value="CHORD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CHORD" evidence="4">
        <location>
            <begin position="5"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="CHORD" evidence="4">
        <location>
            <begin position="137"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8190133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="199" mass="23230" checksum="0616597F89FF4732" modified="2022-10-12" version="1">METKCKRNGCKKPYNENENNDQACKHHPGKPIFSDLKKGWTCCNKIVYDWDEFMKIEPCAFGKHTNIDVTEGGEFYQSKTVQNAQKGIDNFVDNTPQPVRKIDDYNKEEAEKKKLLEQQQAKVQKQIFVTPTGKYKCTNKGCLKEYDPNENTECLYHPGEPCFHDLKKFWTCCRVEKYDWDEFMKIPTCAKGSHIPKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WLH0</accession>
    <name>A0A8S1WLH0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0940037</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WLH0"/>
    <dbReference type="OrthoDB" id="5065855at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="5">
        <location>
            <begin position="206"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8189461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="415" mass="49271" checksum="8E086E934F2DA08A" modified="2022-10-12" version="1">MRLGLKLKKESKPPQEQQQEQPTQEFKFDFVFFNSNSQSQFDNQDSKQFKLIVQKKNTKEQMIIQKDYIYIGETYNNKMHGNGKIYIKQYDAYCNNNYFVFEGQFQNDQIDGKGTEYYGKTNNKKVDGIFQKLSLIEKFQEYNQDGTILIKQQNLQQSMLQQSFIQEDRTNNKMFRIKSGVKQTQQQIQSQKPQVKQNDKWTKFDVELSKDHLSSLLSEKAINDSIIEAYLNYINYMDSEKFFNLKSLPEIQAYKRTLIIPYFLFEQQKLDVLTYFAEFSYIKGQFWQVYQQIFTIVNSNGHWILLEILFEGQDIKMKVYDSCSKKKAIFYVTICKHIWNMIEPLVKQHNLSISWKSKDIIVAECPQLQQPNDSGIFICAYLKYLISNISLDQITQEVISNIRKTELFNIVKKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WMT0</accession>
    <name>A0A8S1WMT0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0880120</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8187046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WMT0"/>
    <dbReference type="OrthoDB" id="305390at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8187046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="428" mass="51263" checksum="50471A6C36EF1098" modified="2022-10-12" version="1">MEQYSQENPLFDLLYILRGGIPQQQFLFHHIFQYFDKDQKLAIKLCRILMNHQNCNEKNQNGFTPLQIAIIYNQIGAIRYAQQDGRFDFTDETLMQLAITYTQLEIVEILLFDEGLSVIQTNFRKMNSQSSTYLKILKRLEKIEIHKVLKEDTSEYQEIIVNEVPQFPNTKTPGKIMSDNLNEYLIPTAQCIQTLEDINQENIQKEKQKKSNFCTQSSCQEYQIQKQHNQIKEIKENHISYQNLFRCEIVVEQLLSVIFQFKYPQLDYVMLLFLMQSKTDFYYKNLDCLIEQVLVPIPNNYLLSYSNFDQTQKIDYYCKLLTKEILQTLHEYKQQLKRKHHHFDNLKALLSYKLKYDISYQGPSKDLLLKFQNSIQIYRCLFNILHKPYMNFNCTFFTLEQFELINKLEFRNSFQLLTTNQTLQTHNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WVW3</accession>
    <name>A0A8S1WVW3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kelch motif family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1050128</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000105">
        <property type="protein sequence ID" value="CAD8193958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WVW3"/>
    <dbReference type="OrthoDB" id="293573at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093:SF18">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="637"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="190"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="640"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8193958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="676" mass="78207" checksum="2250321B4154F5B5" modified="2022-10-12" version="1">MDSLSPKVNSLSYHTNTKMDITTIFEKYKKTHNKLSPLPLNQFYPMAINENCDLLQMQPDSDVQNFVSFRELNQGAVSELKRSLQQSKMVWNNYSKYLFGEPSLDEIEMDSVDQQQFYTSRKLQLIHNRETFHKMRKQYKQQEEDQINQVKQALKKIHTQTNGIKVYPSLAYQDQQKLELLRKQVRLGGVQQLQERNSDAQQLQKRILESQVALNQIKKQFAYAYNYDYNFWGPCSRDGSSMVTFDRKLYLYGGSSSVQQEDLCVAIVDKQFYKWSPQKLDEKSIHGYRVHHTAGIYKNFMILFGGEIHIEQSNHKLISECTSDIKTISLTSFDIKLIKQPGTIPPRKCHIGEIIGRSLLIFGGIDNRGNYMKDLLCYDISQSKWVSLNVDPFDLYANGIAFHKSCLVTQNKFCDIYRQDPDLKFSNQGIYVFGGQDKFGNYLDTFIRIDVYQKPIRIEQVDYKGSGPIARCQHSMNYNEVLSAIVVYGGKNDDNTIEGFLNDLFLFDVKNSSWIQLELKGAQMPGRCGHSSSCIDTKIFIFGGYNYNGFVKSDVLVIELDSNISHQLIQSDKINEDKPRNSKIRVSFHQLPEKRKTIDKTNERKKEIEKLNFKNSHSFLPMPRRVTMLINAMRFGQEPKSNTEGSGSNSNKNSPKDGTRRPHRNMTMIIEGKQEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WZC6</accession>
    <name>A0A8S1WZC6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1050094</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000105">
        <property type="protein sequence ID" value="CAD8193890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="300949at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="122"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="225"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="255"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1146"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1346"/>
            <end position="1364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1481"/>
            <end position="1506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1732"/>
            <end position="1755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1103"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1103"/>
            <end position="1113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8193890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1789" mass="210100" checksum="5E0726B681C4A92C" modified="2022-10-12" version="1">MKFIIFEQLSCLAHLTHNTQPSEFWIILIVLITNLQYLAIMLPISGWNLWKYSNGHFYLLSKLAQASLIIPFSFESNFNFGDVVFGILILLLFTFIIIIIILVILGIQDDINPLQHYQLRKLIFYFIILSTTIFQLPILFLLIPFIMIGRTNFNQELTIFNVKSILSLVTLFIFSIFVFLQQYFLRAYTFVPFNYLQQKFNGLQSIQYCLSILITILYLVEYDTIVQYIQILIIFLVIILRLVESFFMKPSIPLVNLLQFSFNFTLIAILIILSINIFTHNEYIFQEDQLIYVLMLLGPMAFHLSNQFYIMKCLSVFDYEVTPFTCIHYTQEMHFQYLITCENQKNKEFFQLYQYWKGHKVVCQKHWLKQNYKTDYNQIQKTHQTIFCILNTELERAQQDSRTAYEELQLLYISYVAQFCKKPLLAYVELKRYQSKQQIQSFYFISIRYQMSIYLQTLIKNQQQTNQQIYGQSKVAITERQLSIQSIFEALQFQDQFIPSVIELLNDKINFWNRQIKGFNNIYELERLAIYQSKKIFNLANQINKYCSIDIKNLDHLSISNNVQDLKLISIFCSAIMNDYYSTQICENAISEIYNIEHTLQEDTMTNLSFIQERAVILMLSLVKNRGRIINQDKKMISQFFNYNEQEFAQIDNISGLMPTFFAEKHDKLLLNYLQTAKSQFFDDYNQVFGKSKEGMIISYQLKLDNNFSELDDYVLIGCLSQIKKSADYLLFSDDGFIIGMTENFYSTIIDSKDQSYRILHDNLSQLNAFFLIPNMMELLNELKNTYGKDSIYVQQDKFYKIWKYHNNKDLYDMSIKFGYSSKQNSFINDSSLFKSRASFASIEKEKADIQIYQQLGQSIGENKKLKGVSILQGLTIYSVAFNNQLINSLQILEGKPKQQYIAKINIVYKIIGKKENRKSYFILEINDLRKADEFFKTNDSLMNNQHTFSIQTTNKGNMPAVQDPSESYLGSLNVDKEEVTPNISKQFQPGFQRLLNKFEGKNQAQQNYMDLLGIESARDSISYGPLSQRINFISPSSKQQHMQELIDKDFMDIQNQLEVGNEDIGNEIEMADSSKVKSNSKDQNQKSNNLIEILQNNRLNQKNEDLDREAKKSKSSITSGTSGVSAMSTIKKFQSKTEMTNSLRILVTINIIIMMIILAYIVAHLLKISSYNNQTQQTLSNINGPTLFNRYFFKVFTYTWSLVFNSLGIIESSDFLITQTIAEMQDLASVIFDNLRNMYDTFIGIEQSGMLSSINLDFLYQNNENVTYTYFINIISNVADTLFQALNYNIQTLIQLIDRNYLDNLLMLRYNLKNVVNMNFQLIDSLNELFFQQQQSQLEEFQTQIIIEIILLVIVINVQLTYWRQIEQYSQQILILVGRLQLNQAQDMITRFTAVTETLKQLSGKYGWKKQNYYKLLFFPLNQIGIEAMQTQSRVLKEYQLSQVGMQSIKLNHIIEDRKNYKKNLNVVLNSKINNPNTTIWSATIIIMITCLIFLFYLLGGFLLFKKQQNDLLPTQQLTLGFIRFTSQLDIVVSTAFITKTQPILYDQLVDMKFYSDKEMEIFRDQRVIIQIFISLYSIYFENITQIYENIIESNKITEEDENILLMLYSSDFCELLSEDIPFCNYDNTTNFYELYGKPTRLDDNRDYLSKGIQGVVSRLDSFFKQNYYYETNKVDYYPDLDVVKDLFNTREFTNTLIEHFLDTTDGTNLFIDTIMKSVINITQSDLNLMYTYYLVTGLMLLLFYLLFYGCWIYRTNQRLIQLRLILTNLPIEVLTEQHTLSLLKKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X5U6</accession>
    <name>A0A8S1X5U6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1050087</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000105">
        <property type="protein sequence ID" value="CAD8193876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X5U6"/>
    <dbReference type="OrthoDB" id="441446at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11010">
        <property type="entry name" value="S1-P1_nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.575.10:FF:000003">
        <property type="entry name" value="Single strand-specific nuclease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003154">
        <property type="entry name" value="S1/P1nuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33146">
        <property type="entry name" value="ENDONUCLEASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33146:SF10">
        <property type="entry name" value="STRAND-SPECIFIC NUCLEASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02265">
        <property type="entry name" value="S1-P1_nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035735684" evidence="3">
        <location>
            <begin position="16"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8193876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="306" mass="35028" checksum="248A24F5F4B4FC6A" modified="2022-10-12" version="1">MKGFVFCYLISVVYCWWEVGHMMTAQIAKNFLKDNRPDVLAWADSLVQDFNSLTDGRSNTFAEAAVWLDDIKETGTGFLNDWHYTDRPINPDGLLIKIDDQGRNINSIYAINQAVSVLTNPKTSKNRHTVFKAQMMRVLLHVIGDIHQPLHDTTFWNSSYPNGDAGGNFMKIQLSNGTLMNFHSFWDSGAVSFAPNNSFMARPLSQTDSEYLDKWSKELMTKFPKSKYSNYDMTNPSVWTYLGFRQAQQFVYPMVATSNSYNKDYEKQAIEFCEENLSIGGYRLGAKLIEIYDQIIQNEAKLSISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X6U3</accession>
    <name>A0A8S1X6U3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1140038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000114">
        <property type="protein sequence ID" value="CAD8196951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X6U3"/>
    <dbReference type="OrthoDB" id="309853at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8196951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="305" mass="35740" checksum="83CB67F2A88DBBAB" modified="2022-10-12" version="1">MSKIEKQIQKQRKLSHEFEQTYNSTEIHSNLSFYDEERSQDFYDDNSLVEEDFQDIWEMSSICTENSLNPHSIEENPKNKRIRVSPHPELAMKRGTSNNEGINYNAAESITKNANSGKGIAIHVLGQGSIGTKSKKDQKSQKLLNKQLRWNNDNNNNNNNHDNNNNNNDNNNNNDNNNNLHQQQQNQYQQNNSYYDKQIKQSQKITLSYLLMEREIEKLAKIKAINFIEKYMVKCNVDEVVQMISETYKIMLIPDQQLQQLISVLNYEDRQFQFNLSLQRLSLSRNVHPLASKADQKLRKFFLSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X8A2</accession>
    <name>A0A8S1X8A2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1120081</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000112">
        <property type="protein sequence ID" value="CAD8196346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="296301at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2734"/>
            <end position="2757"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2772"/>
            <end position="2792"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2817"/>
            <end position="2839"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2892"/>
            <end position="2910"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2956"/>
            <end position="2979"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="633"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="665"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="903"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="974"/>
            <end position="1014"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8196346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="3117" mass="360760" checksum="C774D82DB9BA9BF2" modified="2022-10-12" version="1">MIFIICCYQGMSSCVFDTITKPDTEQRIIEQNLSGLGTKSYGWGVWTKYESGYGSPEQLSQTISSMHVLTEKYTERIILSYFIVIDREKLIFYHRLLIQHGGVLQIRELKINVAIANKKWVFFYFCFKETLKSYSMFLYVDNDVFHVSMSAGGPFDIDDLIKQYHLGQIVDVINPFDESINYNLSVFIGLISDMDLRTGESNYYPDIQSFLDSLESVECIKELECSGVSKIINFDNAIFNDQSARILSKLKYENSRFFISGWVRVSQVPLYQQRTLALFRMTLKNKYEDDQYYGDRALFWQYLQNSDQPDSSGLIVTTYHENDPFIPYTTNHNDMISYLSSYYSKAITEWHWFIYEEGRSGTNNIQNTIFWGNGGYNKKTDVNQLQQTHLFETSLYMTLGGDKFYNFIGQIQQFEFQYCHNNDISYFIKCHYSCQSCFGPLETHCITCPDEETSNRVYNQGQSSCGCKGSYVENEDTQCVKSQEYFQDVTILEGEDSNLFACGLGQFYVQFGQVTYCADCPGNREPDFINRRKLFCVDCLNQQYTWYSNPICTEDYEQLISSSTSAYQKKARIPIDYEYFLINFNAQMNQIEIELCQGCLREMTENSDYIVKLKLDQKIKIVCKTCYLIIDGECINVNSNCNSCDSDKCIECSQGYTLYQNQCYKCPVICPNDCIFDGSKFGCLKCEIGYYLDTVNNECLQCGANCLICQPGIPMDGDFVQMQCFKCIDNKDFFIDADLINCKPKNMPHCIYQYQEFSQRSLFENDTHQYKFQSSLDLNFSKPSTKFYDRCALCEKGYVYSLHEFEQGKDKCILYSEITDQDYPNFNSLQIAPESEDDHFQIMLFHQHSYLGLITRILMILGDYMTATLGGKVQDVTVICNIDRKVKRSLDVTPQYEKDLLSECTKSNCKQCIKNYVWFNEYCMLCNQGYYAEIFSGQCYQCVAKLHCRTCQMQHKVYKDGWKWEIRAYYRARTCANYCHTSFNAKCTTTNVDDYEVYCTSCEEGYELYNEKCIPKCSCSKCIIQNDLYICVECPSSLINTNQPIQTLINNKCTDCPANCALCRELNDDEIRKINPYFNPQYSHLDIYSKSCIKGYEPDKSLMQYQYIYRDPILSTQILCQQYQKCYQYLEQEYTIYCSQYAYDYDLDSSINKNAFRYKNMALSHLFSETHFSIESEKLFDELNKKSIKSAKYILNFKYYNDEPCIIPPNSVIFSNLRRNVFTLQQLEIILIGDITKQTFIQSTFYLEDFTKITLQNFLIVKSTKQSSIGITQLNRVSVNLENFIIKDSDTYRFQIQIKDPLNVQINKFQIINSKIVDTQGIIAYSFSKKIIDRFEFLANNITLLNSEFQNTQIILQILQDNCNNQFHEITQIKSIKNKFISSSLFETYFPLSERKSSFIIIGFSAARDTLTKSGYAILQGALDVKLQDLTIQNSAILEGTKLFQLPLFVIKNFFFDSNSLLSNDNRVITNLINIVYSDLDSVTSNILTFDNIKFQNNYYVSSKGFIEIIQSSNFRFLELKITDLTLIRNDMISEKSSVMKAITLENSTIYLDITSLQLTNVFVQRNLNLPEFTIKNAKNVIINQFTGQLIKQFNILHPSKSCLFSVDEIRQTTMLFFFNILNLEMSNIIMYNLTSINLPLILIKSIEKQNKRQQEKIFLQNCKFVGNILMVSKTSEQLGVLAIQSEQEQTIVFSKLIFEGNIQHSQIDNIAFISSSTILLITPYSTIEMRDSVFYKNIVTNGENSNLILIGKSVSLMDSQFLDQNNIEFQSFSQHLNWGFQQNDQVYLEDLIESFPIYSKGGAGYIKGSEILMDNVYVNNTQALFGGAFYLIPQSMGSLKLMNSQFSNCKTIETNLERSQGGTLYIDSSQSQLDLLIQNTTVTNSYSRNEGGCIYIEPSRVNSLIQFSDFSIQNIYSLLTPFLRTPVSFTSSSLLLMIQFYNVQIINTYSGYLSYLEEIVDMKQTEILNQKNNYLISIDQGNVTMQNCLYKNIFNYGFLNCQGCQSMKLKNVEIKNFTMLSESIINIILNKKYQSTITLNEVSVDNVIEFHGLVDIPEEKVEILVNTVYKCNQNFVIPQIMDVYYSNKRKQIISLYNFYKIINGRQQLITIFKIDQVSILHYLFFQTFNMEQNYCSNCESSIFQITNIDKADSINLVYIIDSTFKNNTCGKFGCVVISSKDVKSIQIQNSNRILESDQTISNIHTQVKFIDSVFIQNKATYGGALLISQINALINNCIFNQNAAIQSGGAIYYQQYDGSKLHLYNSLITHNNANVGGGLYLGNYQMNDPKTLNNYLRNNKAKSFGDNMADNPSQLTVQIGNQLLLTKRIINNNEQIVDQVIQSNYTIGSNVYKYIMVPSGQIVSLYNFFDENTQSFISYNFSLRVIPLNKQYDRIKNLTGTECSLKSRQILDKKQETYNSSLINLKTVIFNETSQDYNLDSLIVYFNPDLNYIGYLQLEIQCNSISIGLYSQESPYQLTSFQTNYSLIFDLQTFPCQRGEYKKPDGTCVLCDSQFDQYNIQAGEQCLIKNPFTMDEVNAARVKLKPFYWRPYEYSHAIEYCQNMPENCIGGWNPGNNLCVNAHVGALCEQCDIYNVRGNGQYSLSSLYKCGSCDNIGDNTIKIALISLWTMISILISVKGTVETANKIDFDNKLSKFKIFAKNPKEGFGGVLIKVLTNYLQIVGAISTFQLQLPSVLQSSIRSVSNPVESMSFSLDCFLITISNIDIIYFRMIWALIMPIIYIISFLSLYTIWILISPTKANKSAITTSAIYLFTYLQPTLLGGFISLLSFRKISDIYWIQGNVAYRYDTQSHFNWMITFILPSTLCLALVIPIYMFLSLYQKRNKLTDSETRKIWGYLYNEYSEKAYFWEIVKILEKGFMIVFLTFYEDLIIIKAAMIFIITFLYSILTKKFKPYKLSYLNLIDEISTLVCETSIVLGMTIYSASSSDNQEIIWPFYIILISINSIFIIIILWEIILAQLEDQQENIDKIREKINKKFPNLQQKNWFFRRLLINRGEQQKRVRDRFQKIRQYLMEIVRANPGRYKQPSIQMFESIFNEKKKDQNESPSDIGNREIKNNLNSISKTKVNPEIFIVEKIEKANEYDSKNRIEKNYNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X8Q0</accession>
    <name>A0A8S1X8Q0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1150068</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000115">
        <property type="protein sequence ID" value="CAD8197362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X8Q0"/>
    <dbReference type="OrthoDB" id="292862at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="519"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8197362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="552" mass="65896" checksum="FF553DA96A81B5A2" modified="2022-10-12" version="1">MNVFQSNQLSPFMNQKVVKKPTVLMQPNQLKKSHVDAKPIRFLQWDDNAKQIKLCLDLKKYNSLFRTGPASFIVYIAARSEINQLSKLNLDKSLPLIDTTLQKGIYIWNHVIYNEHKNIQLYQLLCILGEQFKEKQLRRQSLNLVENSSIFIHADTILMTIASILASEIVFLINNNDDEILENFHQITKHDSNPNYKLQVINLVDGLAQSIDQYKWYQIYDNIKITIMEEIHYLNASSSSFIDNPADTLNNWALELEMSLQKKIYRSYEIDFLTFFSIAEIITKSSMSELEKDNVIENIFFKAIQKQIKYEKDYIESIYQKEIREFCSISEEVTVDSLTYKLNQMRDQAMSLYYIQLGQFYDNPEYEKCLQIIQQKIDQFEIECIRYNFELMDIELNQQFDKIEKKLKDGFPDSYTQFLTDILKFREFRGLKYKNIKQHLQSSYKSKLYSYITEYDKQSKILYEQRMQTQNEKQYQFLKKRDKEVMRGLEILQNYTDLMRDEIGTLKNLIDFNNQINIVVKYKQEIRKLDDEIQQLQLQANKIQIKKSNREF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X9X6</accession>
    <name>A0A8S1X9X6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1160159</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000116">
        <property type="protein sequence ID" value="CAD8197880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X9X6"/>
    <dbReference type="OrthoDB" id="296558at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8197880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="308" mass="37334" checksum="050132EF89558C0D" modified="2022-10-12" version="1">MKQISESAHILQKVYNPKKMNKLFMTTHYQLQNETDLIFEKFMVMPLFGLSVANGISSNCIKAKYLCSDYKKQELYDCNLILIMSAYSDQAVYRSKTMYSKRDGLEQIFKYLAIPNQTYNIHISLLSYFVPQRVFYKQVLQALNIFELLDQKHIEELTKSSSIINQLVDDDNLDSILFKNQEFIDYQKWRKLLKNNAIINLKTLHQHKLSQQIFCQYFFRYHYYQGCEDEIIKLNKFLVDDFDMFKFRFRLEHNEKKMKFYFLRMLSYFKLNEKLEKFLKFSFKSYSLDWNKELLREMKCSINKNKNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XAM6</accession>
    <name>A0A8S1XAM6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1170041</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000117">
        <property type="protein sequence ID" value="CAD8197995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XAM6"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8197995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="265" mass="31062" checksum="6B610D3E180EC0B7" modified="2022-10-12" version="1">MDTIDTLKITFLEFINKLNPLLPEQLLSIVSEFKEQLYSKTPTDLYNFVQQIPELKKSDSSSSNISPEPLLNKVASHGYNPLSICSQKDSDDKSQTSYSAEKYQQLKIEINKNFFLKPTTKFNTNTQNKISKHVDIEAKVQRENIKSVDTLREMPTIKRISFAERTGLLNQISESLHQFKKDCITINTVYEKSSEISTPQTPNDMRVGQQQYFYYQQFPQQPVCQFKGYSVYSKRGFMQPIQFDMQFQLMQQQFQNQLIYKKLYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XHN1</accession>
    <name>A0A8S1XHN1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1240063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000124">
        <property type="protein sequence ID" value="CAD8200324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XHN1"/>
    <dbReference type="OrthoDB" id="303889at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8200324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="142" mass="16565" checksum="BB58B3B0D46CC5A3" modified="2022-10-12" version="1">MYNTEFWVKYVFRVLHIGSVTALGGRIIYDYLWPDQAEITKAQILFAGISGFLMILAGIVNIFLLKGKEKLKSKNKFWAGTLHLKAITTIIILTPLAKYISRDPQIVKAIQFYYVVAMLLLSPFLRFYREWWTELNRQNKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XJS2</accession>
    <name>A0A8S1XJS2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1260151</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000126">
        <property type="protein sequence ID" value="CAD8201107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XJS2"/>
    <dbReference type="OrthoDB" id="291360at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048678">
        <property type="term" value="P:response to axon injury"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052315">
        <property type="entry name" value="MORN4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46614">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46614:SF1">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8201107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="381" mass="43935" checksum="2BE7E034B2FA50FF" modified="2022-10-12" version="1">MNLYSEECVPQNMSQDFIKRKYRGKENMSLNRNSIKMNSITQVIEKSQQFQNLKLTIGEYWGQVKLNRANGIGSCHFNTGGFYYGEWKNNIIHGIGIYFFNVGGYIKGEFQDGKAEGNCELCYQNGQLYQGTFKQGKQQGLGVKTSRRGKEVIQYKDGIRINVNEEHSQKESINQLLKIQLPQIYIHQDRTVYGLQCDLTGLGVIQYKNGRVDMGQFQNGFLNGKGRIIFKSGDIYDGQLKNGQFQGQGFYLNYNSKQMTEGIFEKNQIVSIENQIIQYPPKGDDEQFVVDKRINIKNLHLKSRGYNNINSQYNSEKKPNKVNKIQNLIQHMLNPIKNSLKSESVQNEEVLDNLLSRVQSPQQRKNLEQIINQVTSPQRIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XL68</accession>
    <name>A0A8S1XL68_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1300076</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000130">
        <property type="protein sequence ID" value="CAD8202170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XL68"/>
    <dbReference type="OrthoDB" id="406044at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF263">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8202170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="309" mass="35075" checksum="7494584D23241D2D" modified="2022-10-12" version="1">MGICAFDHKSAENQELQQQAVDLQLDSNGKVLQTQKQKTLDENQAALKIQNSFRIKQAKQEAEDKKNELVLDLPKDWIQYNSKFIIPELPKQFKENVQKSLTKQDRLLNSMQAPDGSVYFGQWSRGKQNGYGQMIKPDGTYFEGFWTQGKFQHGGILFSNGDYFIGTETNGEKVYKNGIIYIGEVQLGIPHGRGSLHYPDGSTYEGEHNNGLITGIGKFRYQNGTYYAGQLLNGIMHGKGILIETDGNMYEGDFVNGQKEGKGILKFPDRSQFEGYFKNNQRHGKGKLIQRDGQVKDCEFQFGEQIYPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XN70</accession>
    <name>A0A8S1XN70_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1300063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000130">
        <property type="protein sequence ID" value="CAD8202144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XN70"/>
    <dbReference type="OrthoDB" id="288930at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="539"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8202144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="589" mass="67769" checksum="275A3A6CA87195D9" modified="2022-10-12" version="1">MFLFILVIVFQVIGEELLNLTTKTFLIESEVSGVKEVIQGSRQFITDFENAEFAINIELQGNNSYPWDICFSKQGSIEFAEYENHHSIIYNATHNPDEEHANDPSYYLSTPILVNMVNTNQGLLIVTSDHTLLNYNVTQDPQSIIGFKSQLLQEYDYDSLRSEATEAEVPQIVYAPMFEYVYLIYRDQILKGKVQPNMTLQNCSIGSGLENDNNFIGQIKILNNYMFVPLGRDGLDIYQINKDGDISLKLSMNSFDLYNKDLIISLVDIVFSQSSTSNDTYAFILDSIHGVTKFLVKTSEKMIVLNRDDNFGLVDIKSGLAIAVQSDDFLLILKEVGINQQLVEIGFKDQGWFEVKTHFLTGQYFDIILSKQFVLLRGKDEHRIVRTGVYEEFEPEFKFYTQDDYYTEKANSFYEEYVFIPKLQNVQFYESDFQTDGTPNQWSFQKSYPYLLGLTQHTIVELPYIIRNPMIYCNPKSEQDIGNIYKYDILMNATSCPEKDKYLEDNPTVPYQTLQCSYKNTFQVKVVLAQAKIYNTQELIGVIIGLIILLILLAFLLAYLYRKYKVKEEGLTSQGYDLEPNDAPATQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XQU7</accession>
    <name>A0A8S1XQU7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">IQ calmodulin-binding motif family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1320119</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000132">
        <property type="protein sequence ID" value="CAD8202932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XQU7"/>
    <dbReference type="OrthoDB" id="190375at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051295">
        <property type="term" value="P:establishment of meiotic spindle localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007051">
        <property type="term" value="P:spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.190:FF:000001">
        <property type="entry name" value="unconventional myosin-Va"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051185">
        <property type="entry name" value="ASPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22706">
        <property type="entry name" value="ASSEMBLY FACTOR FOR SPINDLE MICROTUBULES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22706:SF1">
        <property type="entry name" value="ASSEMBLY FACTOR FOR SPINDLE MICROTUBULES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="208"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="126"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8202932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="233" mass="28216" checksum="B12115ECAFF018E9" modified="2022-10-12" version="1">MFNKKKLLQNIIELYYDQVEQMESNYEIDNKQATKIQSYYRMYVLHKSYKKNQNATITIQKYCRAYLARQLVKQKLSESRNRRNIIYLFHQAQTIQRYFRGYIYRKYVHDFYLRKKQLELMNIKNQNFLQELRSKAEQEKEEEKIRHEQAAKQEFMKLAINLHHLTSTKSQPGIYKPPFAKENPKAFDIEVETQLKIAFQTNVQWKTQKRSGSAQRAVTSQGFRIRPQSQKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XRS8</accession>
    <name>A0A8S1XRS8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">2-oxoacid dehydrogenase acyltransferase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1350038</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000135">
        <property type="protein sequence ID" value="CAD8203800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XRS8"/>
    <dbReference type="OrthoDB" id="196858at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="2-oxoacid dehydrogenase acyltransferase catalytic" evidence="2">
        <location>
            <begin position="222"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8203800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="308" mass="34223" checksum="64FB736B84445DBB" modified="2022-10-12" version="1">MTIVNNAFIASVLIYSFLFDTRLLYIFLGIIAIYQALSYFFYPSVSYGSGRRRLSQALWSPPTEGVIHNLVEIDLTNTMKYLSSKSAKDSHVTLTHICIRAIAECINVSRKKICGKIVFGKFVEFPTIDISCLVNIGEGDDLAALLVQNADKKTFEDIAEYINGKAKLAKQGKDEEHQQRSGLLKYLPPFAIGLLIQVTSFLTYNLGITIKALGLKKDCFGVATVTSIGTLGFKNAIAPFTPMMRNLLIVTVNQIVEKAVVKNGQIVIAPTFQLNFCTDHRFLDGGAIVKSNKVLYDVFENPEKYARQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XU82</accession>
    <name>A0A8S1XU82_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MIF4G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1390062</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000139">
        <property type="protein sequence ID" value="CAD8204951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XU82"/>
    <dbReference type="OrthoDB" id="347287at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23253">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4 GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23253:SF9">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4 GAMMA 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIF4G" evidence="5">
        <location>
            <begin position="286"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="194"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="570"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="194"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8204951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="790" mass="93065" checksum="34EF58BD24BAECBD" modified="2022-10-12" version="1">MFQNPIIKRELKLSTPFEPQIRQQYGQNYIPTVISYEQNSYSQIYTPTTQNQPGIHKQGNTINSLSLNGNHNLIQNNQTIDNTINGQQPQQIVQKPKKKLVLKSTVQQSFILDQNVEKKADEDQRKLLEQKKLEDERKKNEEEQQRLLELKKPKPYSKEDLKKLVEAINDFTDYFNQDEWKILQERKHFNIKEIQIKNQPRKEGKSNTYTKTPNQNQQRNSNKPAVTVQPFDRAQIQSATETQPKIIIVRVVEDIQTKQLKDRMKSQAEDFFQTLNQTPEQREIIFKEIRYRLNQLAPDNFDMASQNIIKLVIENKNENDSPYLEYLAQKIIEKSQTEPKYRKLYTKLCQLLIKEPQLTIYKEKQDGKAKSVSLFKNQLLNQVQQVFDERKNKKEDLSHMKPEEREQYHLIRKSKIMGNVRFIGDLFLSKVLPILAVEYAIRELISDFVSQYQPNQENSEESIEGLIELLDQIGGSYKTQNTTDLDILRKELKNFFDGTTKNLEEAKGFLQKTLEKNLSIEIIMEIFNILICKYKLSQRISMLIENVQERRNQGWKDHYSRQDQAQSAKIIQQEQEKEIIEQNYNQSKNKNKKYQAQQVLYVEKNSSSSQTTTSIQQKQQLFDLNELAIFVQGTFNMEPQQQETKLLEELKRIGVSDEAIQKFFEVFFESLLSYKLMKQNIERAQMVFELLSKIEAKEEIVKKATQKALNEDKVYDLADSPTAIDLLVDIIGYFLNESDINSLKKLYFKPEIDIEDFNDFMSKVATQLIIKYSDKEYIIKEYFGILGYQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XZG1</accession>
    <name>A0A8S1XZG1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1440006</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000144">
        <property type="protein sequence ID" value="CAD8206385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XZG1"/>
    <dbReference type="OrthoDB" id="286663at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001034">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="83"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8206385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="459" mass="53354" checksum="F7AA0DB45B2999D1" modified="2022-10-12" version="1">MDIQNQKRKSKFSFSRAPSNENSSALQQLKNRFKGQMLQLINKNENKDQDKQIQEFLIRINVELEEEDLKYIKFDMSLFHENYIDGEILGEGCIGLVRSVTRKQDGCEFACKIVKTDSEEIVKNMILEFKNLKKLSHPHIVAMKELFIQWNEGFQSTGTVLVIMEKVKGKEMFEVIQKQKNYSEWTARILFEQILVSIKYMHDNYCCHRDLKPNNILCTEDGTSIKITDFNVSKFTDGYKEFTNLDDHGKIEMWTYTGTVAFSAPEIFSGGLYNEQVDLWSAGVILYVMLSGELPFNSEYLNDLIDQIQKCKYQMIGPIWDQISSQAKDLIQNLLQLDPLHRLTPEQALDHPWIKNVQNKSEIPRDTLEKNMARFLNQKINTDSELSAKKIKQICFLFGAGEIWKRHSLKNSSVSKISEDLKKFKSIDISTYKKEIQVTKDQNSNHVYHIDCPFSDDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y191</accession>
    <name>A0A8S1Y191_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1480117</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000148">
        <property type="protein sequence ID" value="CAD8207781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y191"/>
    <dbReference type="OrthoDB" id="307293at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="38"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8207781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="354" mass="43226" checksum="713E9ECAE093E6F2" modified="2022-10-12" version="1">MQISPLNKKINISLNTSRDARTSLQEVISSQTEQIIKSNQLEQELHRVNQQRKLLSMELDDWSYQYVQKSQALQECELENALLRRQVQAHRQQEDISKLHLLLQQKNQIILEWTKKFENIEKRLENENKLQQQIKHQQCVNDIIKQQLTELKQQQMTLQKNYLELKEERNQYYRKFIESSSLIQKLEINNKQIEIERNQLQSELKQFKQDYQQLKEQQYQVQTFEEKKNKWKTRYHNLLKEFKNNHIEQNSQNLIEQHNNEVQNYQNQKEFNIPENNDMEELNDIKDKLQQLEQENQYLREQNIELQNQFQSATDKLLKQQLQNSMTKEQSQTFSFVQYHHEYCDSLEEQQIQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y294</accession>
    <name>A0A8S1Y294_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T1450046</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000145">
        <property type="protein sequence ID" value="CAD8206768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y294"/>
    <dbReference type="OrthoDB" id="301422at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15959">
        <property type="entry name" value="SYNTAXIN-18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15959:SF0">
        <property type="entry name" value="SYNTAXIN-18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="252"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8206768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="271" mass="31795" checksum="CA891263820F502E" modified="2022-10-12" version="1">MDITDKFFQIAGTSSLKQSIYENESDYFNSYTSQIYSMVITIIQFMDIQKDKYIAKSNLYGHPFSEKERDDFDHEVQTKISLCSEKIKGIQEMLKLTKTKTEYAHKEIVLGCLICELNKGILLFKEFKLFRQRQKERISQLTKLSKVNSKIEDKRSQGQSQVSVEQENDPLYLENQQLLKKYDNEMKDILSIQQQIRDISSSLQRMQEIIAHQEKLTEEILIQANESLSNIEKANKHLISATKMNEQFGKRWGLLFLLMGIILLFYDYINA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y790</accession>
    <name>A0A8S1Y790_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1540083</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000154">
        <property type="protein sequence ID" value="CAD8209361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y790"/>
    <dbReference type="OrthoDB" id="292639at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="612"/>
            <end position="639"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8209361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1029" mass="120845" checksum="3E378E641B635923" modified="2022-10-12" version="1">MEDQLELTSQWGFYEMCTKKNNKLPCLIPQTIIFDKLMPKVWLFNPKNTQVVMKKNSDKLSNLEIAKALLGIKFPKDIKIDTLSQLVDQAKQCPQFKEECPIAFLQMKRNEGIPMNRQELLQFFVEGKDLSSLAYMQQYIENQNEVYYVEYHYDVDFIPITFKFFKKTLTIKRINKSTTAKSSNPFQTLLMPVQSSGQIPVRQQDVQKVQTMFKYCNSTLNQRFQEETKKIAFYLEKVFKVKIRKFTPKFIVDYRDQIYFAGIKQISIQIHNGAPYQKGLRDEIASNGQFDNFMMACKQFRLDKNANNQFKKYPNQIVEEDKKLSKTLYKHLILERCQGDFCNYKLIEKNKGPLQLRKGLLETYKNVFKESKQTVSNMSTNLPYEISLQLIVRTRDDHKEVIELFKKYKIHLENYNPEEHPEIKDEQQHPFSKENSDVQALINLYKSVKICKNCFIIYSMIQRHFESQLKKDIKNGVQFINEKKTPSLQIDEEAERRRKREENIIKRQEKLQMKPSTSTKSFRIFRAPTQKRNFLITSIPESRTSKTASPQKFRDVSKQIEINRLTLGYLNYQKLEQFQKPSQMEQSQEFKNESYNLIYLLAEQKAPNVRRIDTNQQENKELQGRAEEIKKMLAKTSKKEQECRAIQKEYYNEKIQENVQQILLSTSSHYYYHVNFDIIRKLYQFQLSTPMQALSYDLKYDQLRALQIEEVCEMMNYPPPCFDITQLEYLVVDSQTAVPYALFESNITKKKEKSSQSGIDLIIILHDMFESFFEYYPIIVDLINDAQNKKILLLNTPGQAYTLFNKKQAYTNIYIAGILDQILYELSIKGKIDLQTDTLKILGVGSGGFHAQAFIVQCQNTIQFSQITFVNSYTSIYPQLRSFFAQSIKIFESQPPDMPELAFEYTNTIMNTQPISEDLLSCVLQQNPINQQGRQIILEGLLQSPSFMERFQKLILSVQVYHSLKNCLVNISEADLLQTFIQDETLDVNNVALSKKIQMNKRTIQYIEGGHSVLYENKDQLVQILNRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y7T5</accession>
    <name>A0A8S1Y7T5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1490072</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000149">
        <property type="protein sequence ID" value="CAD8207982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y7T5"/>
    <dbReference type="OrthoDB" id="10339812at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8207982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="174" mass="20838" checksum="93AD62428E62556D" modified="2022-10-12" version="1">MKEHQGLMGNEFQPIKQKYFLHEKEQIRQDPFVESLQEYFQIFRRPFFFLALIIPSIILQADFIELDVSERGGIFKMEQTWDPPISIQMFSKIDILLLDVQLFLDGKEIEEFTIYEHLYMNTQYNLTSNTNYSFTVRLMAQFDFVPTSYLIIIVVMLVISLLYSIITYTRYKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y930</accession>
    <name>A0A8S1Y930_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1580080</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000158">
        <property type="protein sequence ID" value="CAD8210249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y930"/>
    <dbReference type="OrthoDB" id="10252171at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="59"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8210249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="419" mass="48525" checksum="008589D45B78C14B" modified="2022-10-12" version="1">MTKLNSTLRYQIKHESTSIILRLEKDDCQFKEYLFLDKSEPWELKLKSQIPQLDYQTYYELEKLIGSGSFASVYITKRKTDGIKLAIKAFLKKMLIQKDPEKWRESIDNEIKVMKSLDHTSILKCYDHFENRAQCYIVMDLARGGTLEQGLKKLEEPLPFLTVKVIFRQIVEAIKHMHEKGYMHRDLKPSNILLKRPMALKQFSLIAQTDPNIVVSDFGVSSEIKKDMDVGKYCGSPGFMAPEVILCENNKTLTYDEKCDIFSLGCILYRLITNKPLFNGQNSQAMKQQNKECNLDWNKIQDEFYQNKALVNLLSKMLSIDPRQRPSCEDILNSKILQVQLDNEGCPLFINYKKPKSSSIQQIKTARPSIKSVSNCKLPQLSPNHFTFNNHNNNHINKRSGNLLLPPINSRLQTEQSQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y989</accession>
    <name>A0A8S1Y989_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Elongation factor 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T1580106</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000158">
        <property type="protein sequence ID" value="CAD8210299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y989"/>
    <dbReference type="OrthoDB" id="10365086at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01681">
        <property type="entry name" value="aeEF2_snRNP_like_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04096">
        <property type="entry name" value="eEF2_snRNP_like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16268">
        <property type="entry name" value="EF2_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1430.10:FF:000003">
        <property type="entry name" value="Elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000003">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000002">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF10">
        <property type="entry name" value="EUKARYOTIC TRANSLATION ELONGATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation elongation factor EFG/EF2" evidence="9">
        <location>
            <begin position="583"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="domain" description="Elongation factor EFG" evidence="8">
        <location>
            <begin position="706"/>
            <end position="795"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8210299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="825" mass="93373" checksum="FDA6A5A065131D16" modified="2022-10-12" version="1">MVNLILNQITKSMNNQDNIRNICVFANMDHGRQTIIDQLLNKAGINLIDQSKEAIIINNTTPTNLSLYYEFENQSNGVIQQYLFNLIDFPRLPNFNSETILSSLRVSDGILIIVDYIEGISTSTESLLRMCMKEKVKPVLMVNKLDRAILELQQDSETIYNTLVQIIEQINMIIFGYQQDDMGDLLIKPEQGTVSFGSGKDGWALTCTKFAEFYASKFNTESKKLQEKFWGENYFDSETKCWVNENQSNIGKELKRTFVQFILDPICKMVKAIMDGNIQIVIKMLNVLGLQLNEQDQGLIGKQLLQTVMSKWINVSDNLIQMIIYHLPSPKQAQQYRTSYFYEGSQNNQVAQSIKNCNPNGPFVMFISKVIQVGRDNYIAFGRIFSGTIKQDQQIRIMGSNFKPCSKDDIFIRQIGKTAWINGRMVEYIEQGIPCGNVIGLIGDSNVLTNSSTISDHPECHLIRPIKNSVYPVVKMAISPKNPHELPKLIEGLRRLAQINQLIDYSIEDSGQHFISGCSEAHLQIALNELEDNLNGIQLDKSDPIVVYKETVTASSQIICMAKSENQHNRFYAQAVSLDENLQIAIEKGLITNNPQSRANILTYEYDWNKNEALKIWTFGPNDTGPNILCNQTTAVQYLNEIKESMQFAWQISTKEGALCQENMRGVRVNILDCVLSAETIYRGEGQIIPTARRLYSACELTAQPRLQEPILLTEVTVPNQVTAGVYQCLSIRKGIIIEEEQIVGTQLTRIKSYLSVAQSIGYSSHLRILTQGQAYPQCEFDHWAIIGGDPFEHGSLANELVLNIRKRKGLGNKLPNIDEYLNQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YAU5</accession>
    <name>A0A8S1YAU5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1640058</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000164">
        <property type="protein sequence ID" value="CAD8211596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YAU5"/>
    <dbReference type="OrthoDB" id="10295515at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8211596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="428" mass="50588" checksum="0F10F8892D8C0C10" modified="2022-10-12" version="1">MKLNYFGTTFSSTKTQQNYLRKLFYHYYMEHKKLIKDKSLSLENFMAISSSFIDALERTKLYSQIRLLILPSQKKFPNGIILHFDIQDKLPYFLSASNEIDTQGYQLKMKFGNKNFFGIHDQLAFEYDQNLSTNKSNWSLSWSYKDYKDSNYSFSYRQAQIQQIQNIDENLSEFSFAYNNATTQLTISNQYRFNKIEDEESLQGRYLFRKYVNNLDFLPTQSTIITYSKPFSSQIFQDQGILEGHNNKFNCSLGIPRLTMNPFIKVWMSHQLWIPLYEHRNRIKKIKKLVANSSIQLSFNYGLAFSQSHLDLNDTFQNLQFRGFNQMIEMSKLQNRKYDTFAKFDFKYNIHNLQGMKEKNLSYQLFWYGSLLTFGHPKSLLQNFRSSLGIGVSMPIGGMQLEALVPLYTQKKQFDKDPIFQIRIATDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YD19</accession>
    <name>A0A8S1YD19_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1560003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000156">
        <property type="protein sequence ID" value="CAD8209732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YD19"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8209732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="43" mass="4963" checksum="D34339EDBB7DB7C1" modified="2022-10-12" version="1">MDEIKSLCEQKKYQQAIMSAEEGLMVINNNSHLHCIKGEKEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YH52</accession>
    <name>A0A8S1YH52_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1690054</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000169">
        <property type="protein sequence ID" value="CAD8212603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YH52"/>
    <dbReference type="OrthoDB" id="10043504at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="22"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="56"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8212603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="162" mass="19135" checksum="9175DBB08341EA9A" modified="2022-10-12" version="1">MNRFEEALECYDSAIQKNPENADYILGKAEILKKMNRFVEALQNYDSAIQKNPENADYYNNKAITLISMYSYEEALEYYDSAFQKNPEKAEYYNCKAIALDNMNRFEEALIKNDSAIQKNLLNSEYLNRKVSSERSFCQQQYQLVFQDFDFGSIQQTKYYVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YHF9</accession>
    <name>A0A8S1YHF9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1930009</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000193">
        <property type="protein sequence ID" value="CAD8214006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YHF9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8214006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="112" mass="13285" checksum="26C2E104F5B45CBB" modified="2022-10-12" version="1">MQINKENYLKNNQIDFLFIDQQEIKIFIKRLLCTVIQIIQLFNSLLFFNDQFNLALFFSNDITSIQQEFIQLNIEFFPALLSELIEDKKPINQGMMVSLSYLRSIKLKSLTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S1P9</accession>
    <name>A0A8S1S1P9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0030141</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="288020at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8134116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2078" mass="235138" checksum="DFC7CB07D878C568" modified="2022-10-12" version="1">MFLILLLLYQVSCGRVRPYGPRSLTMSNVNPNGWSEYNFTMIFDTTLPSSGILVIEFPEANYPDRLGLRPDTILYAPYPLTRTFVQNDRSLEIQLGEWPIETPLTVTISGIINSQKQGGTGNFKVYTKLNSHIVDINRNFAVIGLAGETIQLLSAQMSIDNNYAGEVTNYLASLQPSQDLDSRIDFIIQFPELYDLSQLKKCTCNVFTPICTVHNNFKNQILVSGNQNSISKGTSVQITFNDIVNPSRKMKTPLFNFRVVERKTNNTLQESSAVNGLEILAGQIKLVQLEILYYQPYIYYKRDFRLQFRPKNGFQYAKISTDFYEILSCQITRGLLDPSKFQDSVCSPLGRDMFLSNFQSYVESDYSYEFVEVRFKARIGDRVILTNPVEIYTYKDSDYLYLTDQDTLSINSQLQIVLWPIVNPTLTYTITFDDPLVQTNVYPQTNAQITIVYTAKVGISLTTKTVFGLEVPSDFQGVPECTFKDPNDVMVDVTCDFGLGFLNITNVFDYYDSTIKGAVITIKGLLTPENAGNFPIGVFVQDQNNVYYNNDTFVISPPTFKKTTGAPELTTAVSQLSIHQNQQTAIQIQLQLDCDVSAGVKNMNDPYVPFTTIEVYFYQYYITNNVFEYDLGSGLPDLSQYGCQFQDGFVTSSGNQLSCALFHGLNTATPTFANYAMIKIQGYAAITATNTSPVTISLKIPVKYPSTIGAIPRIRVDLVKYNQKIKSILRSQSFNIGAVKNSVGNALTVFSGPVLENPKLQVETTLTYKFKTVISLSKSKLNGIYFEIPSGFTFNSNKLLVSIDGVYQEPEVIQYFPKFIFIMTKLSQLSNGITHTLIFERVDIPVSLPSSSVTHDIKTASGGQFADTATFTIPTLTKGSLLDISLTLSSDYQQHPYVNYSISFTTSMKSYDGSMIIIDFPLGYDIQASNAYLDNIPYTNLKNSTTNTINYDMPTIRKFRVLNFAPLIKGQRVSIGIYSVKNPMSGTFTIGISTVDKNGNVIEDGSKQVTIGSTIFSTQILTVTSVESTPNNKKALGLYTWNVNADFTIPKLSEIKLLLPGMLSIYSISADIVSCRIVQDSVIPLKECLAIEISTPSYIQMITDYPIKGKPFKIEYYGYLQLDNSLSGFTISITYNSQVIATGLCPTVAVTDQITPSKVILDFYPKNEGEIAYYNFNLEITSAFTLTTNHYILIRFPFEYDYIIGIEKMQVKSNTLQGALKMTIIPYHIKITGFYQLTNFIGILDISLLGVINPNRLNNQATGTFLFGIMDGNNMVLGNTAIDGVIPQLAPLLIQLNKITSSVSLSRYASTFTFEVYPLQKVLATVYGGQLYVDFPNNFIIDQFNGGCETTSQFSFFASCKWDYFRFLFQTNNQDWYADRTGQLDLTITNVLTPDDDGETNNFIIYNYDSINKIVLGRTYANLHSASLLYNYDGLQIDVNLKQPFILEVGSYSDEIIMNITEKADFTLTLSPNVLDSQIIITPNPVQIVAGSKQAKFRVAAPRTILLQTYYIQWSKTGDTLPVTYAPLRLTPLQMVKGTLKRKLTIEKMEYMPRNGTSYPLYITTNNPPYQQLLIRIGFKMNITADVPLRQKWFITDKRLEQLGLTYAELVDSGIDVTEHSIANLSSTQLDMAKLQYSVNYDLNISIDYPSNENTITLYYRLRGYDADSFELDVQEMIIPIRDQEFRKPKILETKVLDVSRSTATFQVTTDIKGFIFYVYADQHMPNPIFEETKVPNLDNVTLSYSNPQYGIDYVRTMTLDVNFTISKLQPNHNYEIFIFVMNLNKVDNPTFMKVFFQTLKSQRIALLTLKINQAAIDNEVKMDYIQKMGEMIGITKARLLERTTFCKQTTLDTSTSNSYLFWNLMILPDPSNMNEFTKPLILAQTLNSRRSEIQKFITRLDDEPFYTLEEISDDIPSFVINPEMIDRTTSSISLRFKVSYCGKAYLTVRKREMSQNLSIVNETIFAQELKTVQFPSAYQIKNALNESNQPINQTKITIQDTETFYQYNYTDLDQKTVYDIYISLENDFIANPELFSNDSVARFTAKTQKIIIPLNIYSINYQDLLKFLMIINILLVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S7G9</accession>
    <name>A0A8S1S7G9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase M16 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0040233</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Substrate recognition and binding subunit of the essential mitochondrial processing protease (MPP), which cleaves the mitochondrial sequence off newly imported precursors proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S7G9"/>
    <dbReference type="OrthoDB" id="10251424at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF49">
        <property type="entry name" value="MITOCHONDRIAL-PROCESSING PEPTIDASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="3">
        <location>
            <begin position="181"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8135457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="446" mass="51057" checksum="2612EB48E374461F" modified="2022-10-12" version="1">MNKILRGFSTVKSMKFIKDKQPLIKISELPSGIKIFTEQTAFPFASDIGICFKAGLRNELPSETGSLFSLNQHMYHISENDCLENFEQTLRTGCMLDQTYDSELSYWKCQFIQEDFDMIVDVLLKLALTTKRIAKETRDQFNKMSIPQERMTVDEAIKKAAFGTHPLANAKTSAQILPKQEDFSRFQEQMLTSQNMIIGYAGVWSHEQFREYIEKKLVNHQQFFQRQLRKQTSPEFLSQICALNDDQNDSIDIALLFKGNKWSDINMPTQYILNQILGSSSSWSTGGPGKGMRSRTTLNLMQSIQSVEEASGISQVFSDAGILGLRVQGPPTSKQELYSCLIDEFRKLGQPMSDEEFLRGKNIAITLINLNLERQADRLEEQIKTTFLMGQNAVPLYESLIQNVTKEQLQDYVKNLINNAEPTLLYYGKNVQEVPTMQQVKRNLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S7M6</accession>
    <name>A0A8S1S7M6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0050052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S7M6"/>
    <dbReference type="OrthoDB" id="277011at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000093">
        <property type="entry name" value="NLI interacting factor-like phosphatase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="1">
        <location>
            <begin position="51"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8136246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="294" mass="34457" checksum="5E14BF37C4440CAC" modified="2022-10-12" version="1">MVLQQQQESCYLQNYVIKMNCLQCIKRCFKNIRQNFDGQDASFQLSTQNSQIRKKKTLVLDLDETLVHCEFKENPNFHYETILDVWHRGVLYTVYLCKRPYLREFLQQMSAYYEIIVFTAGYESYCDKVLQHIDVDRHISDYFARSNCRFVNGICLKDLSILDRPLDQLIFIDNNPNAFEMQPDNGLLIPSFLDSDEDECLLKLIPFLKQMANKSKLIPVSSFLSDYESIHGVLFNDNQVTLQCQADGDEVSLSEESFIVLEYIQKHRKTQTSQQQQKQFIKTIAQRSQTLFSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S9I4</accession>
    <name>A0A8S1S9I4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0040392</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S9I4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8135729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="80" mass="9336" checksum="3142B1590DCFB10D" modified="2022-10-12" version="1">MGNANSDQFHQNRNQQSKITTNSVQCDTTNSDDFKDLKSIERQMYSITKNPIVQRRKLKQNKLKSNKLSLLTYEAFGAYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SAP4</accession>
    <name>A0A8S1SAP4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0060030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SAP4"/>
    <dbReference type="OrthoDB" id="310534at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8137316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="305" mass="36559" checksum="B4F9727D21DB35F7" modified="2022-10-12" version="1">METDRLRKSIKNQFEQKEIKQKGEFLSIIDQELQSLKEKLATQILQENNNIIKYNIKLLQLEKKNAEKQDIMIDQELQLRNYATLKQQSLQFDVSTQYNEIQQIDKDDQKNEINILNVNVQIEELKTRNLKLSLKLFKLLLYFVDLELYDSEFAKQAALDDFPCDQLYKPKLPEIQNMILNLEVNEKKYQSIIKQKKMKKQLYVYDFSSDAKTDQQKRSIFTLGEQLDDQKYLEQLLQPKRNTQPKCKTLNDIILKKRRQSIDTSEPRLDFYKINRKLIADRFQQQPIQFLSYQQCRIHSLSIKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SC34</accession>
    <name>A0A8S1SC34_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SP-RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0050272</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SC34"/>
    <dbReference type="OrthoDB" id="28127at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16650">
        <property type="entry name" value="SP-RING_PIAS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782:SF4">
        <property type="entry name" value="TONALLI, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782">
        <property type="entry name" value="ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02891">
        <property type="entry name" value="zf-MIZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51044">
        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SP-RING-type" evidence="6">
        <location>
            <begin position="770"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="77"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="77"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8136682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="929" mass="109469" checksum="0DC4376B9F134BCC" modified="2022-10-12" version="1">MEHKKLLENVMIDLLKDSSCPIYQYQDWQLINICQQLQLQNFSNIKDQDFQKKFAQFFINYLEAENEISNILEQSSIKRSDSNKINEDRSNQNNQSLQKSNKQKQDLLDITKNKKKVKLKQSNPSDLLEIEENLCTHSSGNPHQMIPQAQKLSQYSFDPLIDSNHDKNLCKEKTQKITFNEQELIESLIERQVKDHNLEPIPKKKNKSIKDFKNQVQSYPILTSNDFIRNKKQLNVDQKKEERENNRAIVDNKIIGQDSRTNRQNQIQKKLSRAIIDSPLEDGEILIQISDSESEQQKKIQKQQKYKGKYQMDDLIEIKSNNITQKQNNFRQKILNQKQNIKDQNINQGIQTDKKLYHPQSLFLSNQESSSPEDKIKFEIQKQKQLLEKQYTIIQSQPQQQNSNNYFFNLERNNQINSLINTNNNILQQKNTLNDIQNIDSTPNSDMIIVIPKESSNLSFFNQQFNQNDHSSINLEKNNKILKQQNQYWNQNKHQIKQQQLLENMKDFQSQNQQLNQTNNSPESVILIKRSFEDGINLERLREKKPKIEVNDIGNNNISQYDNEYLHIQTNLNQCNYCKQINGKIKLIDIENEVKICSSCLLEKLNPFKKIIYTLGFLEYQYQQKSQSKIHLDFTITQEQFRNSGLQLDIRCVMMNKNGLTDFTFPNSCTLLINGVIIKEFKPLIDKSCLKKRKDQCISINLDTLQNTYGIQRKYTFTCIEIIPDYKMRQEIPQQIYIFGLFLVQNQKLEQTIHSILNQSILSQIKTDVYKNEIKVDKSKVSLVCQYSFDLIKIPARGEFCQHQQCFSLNNYLDMMIHAEHMKWICPICKKNSITLRIDHYQWEIIKKIQQLNIKVEYITVDQNGTLDSKDPLYAIIQNNQINSYSDLISQGYTHFERCILQIDNDNEIMFSNKIQTQGENEINAILID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SEG5</accession>
    <name>A0A8S1SEG5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0070025</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SEG5"/>
    <dbReference type="OrthoDB" id="312599at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="32"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="305"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="305"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="335"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8138258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="587" mass="67144" checksum="8DCED8A88E08CE33" modified="2022-10-12" version="1">MYLAQKQMYSQHPSYTNGSSQNVKKMIENFSYGLHQQIGKGFSSKVYKGQNEVTGDTVAIKVIDKALLKTPLHHALLQSEIEALSTLDSQYIMKLYKVFQTQNNTYLITEFCENGDLGSKLTKIGKFPEQQAQNVIFGLVKAYKLLKQNGIIHRDIKPANVLLSSCGTPKLADFGFATTPNSPPLLPNVNVGSPLYMSPQALKNNKYSDKSDIWAIGVSAFEIIFGQVPWQATSEKELAQKMVSVPLTFPSSTKVSQECKEFIKRCLIVNEQERASIDELEKHPWIRGPELAFQVNKQPALMKQKTLEQPKEGKENSAKKLPGDSIHRSGKKHTTQITKPLGQQSKQTETSSFEIKSNTSNIEYTIMSQINFCKYLFRVANLIESTNLFRTDLLRDKLLFFAIKNIMIKMYKLKDIDSNSLDIQGFDTYKSSNQFQQTQITIQQNFTKYQSYFKNIWSLLQQNKNELSIDKKFDAVFDENFTEFESFYIIFNSVLRQAIKELQKEIDKKLGSDDLSKSLPVDMEKDVVLLDYLVTYFQLSQLTLETFNKPYEFAQKSKIEHIAEGSPVRLTYGHFIEIKNKMSDLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SEV8</accession>
    <name>A0A8S1SEV8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0070084</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SEV8"/>
    <dbReference type="OrthoDB" id="301233at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="269"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="328"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8138376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="494" mass="58806" checksum="A49BEB6B1E4DA7E2" modified="2022-10-12" version="1">MNKQEFQTLEENKNNQIYGVAQLENERLNFLWGLCHKGDLKINNALNIMEEEKNKEEIWVDQQIQEKTQKLKNLRLQFQDFSKVNQYDVTQDIKNIDLSIKNIEDKLQNNRKWNFDQNVKFTLQSIEKLNNLVSQKDQLIVQRNLEQYKDNDENQNYINYLLKYQQLNKNIYQKYNFYKKIQVLNKIEKVYASSFNHNGSTLVTAANDGLYFWKFQENNYTYLYNLCTGTLQVEIIFSKFQDLFITHDLIGQFGIVKMINGKWKLDQLIQGHQDQICSIMLQQNDKQIITASQKNGIKIWHLNTFNSWICQFRFEFNSNNLSYLTGNNENYLVSGSFDGNITIWQYQDTNVQNIIIKKFQRVLNAHQNIINIIAFINKYKFSSLSYQSEILIWEQSQSNHLFSKKQEIKFEGLQYWSVFTQRLIFINDLNVLFCCTGNQIKIFKLNIEGKFDLITEIIKEKEIYAFNVTQDGMIFTYSNPFSQKLVLKRAVEIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SFX6</accession>
    <name>A0A8S1SFX6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Casein kinase I</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0060356</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SFX6"/>
    <dbReference type="OrthoDB" id="286981at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000616">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="13"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="332"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8137912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="512" mass="59471" checksum="F398DACAE992AC5B" modified="2022-10-12" version="1">MATVQQNILNNQFVIQKKISSGSFGVVYQGFDLRTSDHVAIKIEKPEIDDLFSLDREVQILRILHGIPQVPKLKWAGKDKGNNVMVIQLLGRDLTHYLRQRKRFSLACVLGIADQMLTILEAIHSKGIIHRDIKPENILAGKDREQNIIYLVDYGIAKQFKDKDDKHIPLRENKPFLGTSRYASMAAHRGQELSRKDDLESLGYMLIFLLKGSLPWQSISYKNEDEKVKMVGLMKMRITSQELCQDIPTEFMRFIDLVKKMSYREKPDYRYFQQLFRRVSIQQQIESRFDWYDENNYEKKSGSTSPKKQQSIKILDQSYLIQQIYTPNKKKLSEEFDDTGQSKRDSNGRQSLINNNTNNLLLCDSRMNLKNKSVSSYNGSNLQYSDIQPDLSCLIAYQRNLRYGSFHNEVEIQKQPDRSTSAQDKIKVKHKLSLDVKPNPHKSIIEFQLGLINNPSIMDFTVNSQNQMNEDESPCLDDKFSILKISSIDNKFKNPIQIFRLNLKDRKGIKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SFY2</accession>
    <name>A0A8S1SFY2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0070318</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SFY2"/>
    <dbReference type="OrthoDB" id="311967at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000457">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF5">
        <property type="entry name" value="MAJOR FACILITATOR-TYPE TRANSPORTER HXNZ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="194"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="302"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="346"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="377"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="400"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="434"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="462"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="24"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8138798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="498" mass="56206" checksum="6322C2D8A810C475" modified="2022-10-12" version="1">MKLLIIKVIKNLHTLMKFQRILVLVINVNINTFLGKYQIYVFIIVGLIGMCDGGETQAISIVFPILEKEWNVSDSQKSLLGSLIYIGYFIGSLFSGVFADKYGRRSSLILSSGIMFICAIIGAFMPNFISYMIFRIFLVTCVGFIIPVSFSMLAENTPLKSRGIVLVTIGFFYTAGELTVCLLTYIFMPNLVSGNWRAVLCWASAPALLTFLISNFLLLESPRYHLIKGNVVEASQVIKKIFQLNNKEPIVIPITTYQNISLELIKQEKEEQGLQEDLKDKSFLESYFIQFRKLLKNQFIKITLVVWYQWFVNTFVYAGVTFLLPLTLNKLNPGEPQDDDVEDLKVITLSCLGEIPVIFIAMIIVNIRIFGRKNSLFLSYFGVGLVGLLIAIIAKGGYFFASMIFFLKMFISFSFTVSYQFVSELYPTYMRASGLGFASSVGRLGSIIMPWIVVYINDIGTFLSYGIFGIISMVAAIATLLLPFDTYQRELDKVVTKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SHL9</accession>
    <name>A0A8S1SHL9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0070017</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SHL9"/>
    <dbReference type="OrthoDB" id="309150at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="2">
        <location>
            <begin position="236"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="92"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8138242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="413" mass="48419" checksum="E23423AEF33E9F47" modified="2022-10-12" version="1">METSIHCQLHQEYQVQYIFLNQNNIKFGCIECISDLQDENHNANIFKNIFLLHDIQSSPQKLLTQISNIDDQNLQFYSCLHKKTETIIMQTFQNWENNMRNFQKKIDNIILQQKEFVEQLLINYKNTIKQLQQEIKFEEFQNLIKEFGTANEKLQNDVLEQISHKITQHFESLDKNNAKELNESLKRIKQEYINQAENLNQPDIQELQTSFQQLIKSTNDLYSQMGKSFNYITQMNSSLIPNSYSKYLLGLIKNKLNQDIKQIKLLYLGSRDGLNGQSLWNKIDGKKHLIYIFKSKNPGNNIFGSYISCKVNQDLNNWQSDESLSSFIFSYTHNEIYPLKQSQKQHAILCQPISYAVWIGMSGNIGICNDFINGQSYLGSSYEWDKFENQRSSHLFGGDSPNIEECEVYELQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJL1</accession>
    <name>A0A8S1SJL1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0080407</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8140010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJL1"/>
    <dbReference type="OrthoDB" id="292474at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8140010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="276" mass="32699" checksum="B97D02DA3C8F6DD6" modified="2022-10-12" version="1">MSESSLSQNDFYELKHVRTLKDNPSKFSQQKKQTQFYLNQAMLEKEYIKTDLSCDCTECGKCSQFSETIKKQYPFCETRWQKRKRILKRFKAAINAIIFILSYKVEAIKKFRKRMHILRAVRNLTTLKKSQITASTHLQPQPMISIPHSRQSQRIKTTPDEETFLHHIVNSAREPRKSQVSQYMNKMLKDVIPKSEVHLKPLSYLNQKKIQQNNAKSINQASFSQHNYSQVTTNSQKYTTLHLEQIMNNQYKMNNKDLLKLIDSLKVRHRIIRCKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJW3</accession>
    <name>A0A8S1SJW3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0080462</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8140120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJW3"/>
    <dbReference type="OrthoDB" id="307079at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="25"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8140120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="185" mass="22067" checksum="FD066F6B5340557B" modified="2022-10-12" version="1">MSKLFKYKKVGNSLVVITKDSIELMDQLRTEHSNLCKSKSELEIKKQLIEDKMRELSINDQLDIPKTMYKYPIDSQNDQRTLDEEINFQINDYQEFISDFQENDTKTKKELFNYFSYKSDIKDLFNDPNNTNNLKKLAEEFGIKSNNINKMKQQITIIQNYLQNQSFPSSWNNIYNTELFDKLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SKH8</accession>
    <name>A0A8S1SKH8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0090044</name>
        <name evidence="2" type="ORF">PPENT_87.1.T0090046</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SKH8"/>
    <dbReference type="OrthoDB" id="284007at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8140378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8140382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="316" mass="36636" checksum="A231E1FCAB7989F7" modified="2022-10-12" version="1">MTDSNIICPYCKNNAYFDPRSSSSFCKSKLCNGIVIEQGKITYENEVNLHEADEPGKKQTRFGGYENKRDAFKNQLCEMLGKSDPKQRKQFYEKFEAGEHQIQKLSSIFRMNEAFTNDVISKYRSLMFDNANEIANKSFLALAALAYNLTIKEKRSQRNYDERLNKIKLEDIVKFCQVTPQQMDLVSKALNMKNSGDPGKIDICQKIVIKVKEHKLPFNEIENMAIYDIPHRLMDNSIIRGEKTSTITCVVEYMVSQLSGNPELNKFTLDQFAQMNDVTKDTAKKLWNTIIENQYWQNHISRWPGQRPISELKHIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SKU7</accession>
    <name>A0A8S1SKU7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0100013</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SKU7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8141245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="67" mass="8164" checksum="1CC52B136081B9FE" modified="2022-10-12" version="1">MDQKIYQFTLIFKLKNAIKLYQEFLDKFEFYRQVLGLKQFETIMKRDCKAIQKLINYSIIKGNLFFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SLW9</accession>
    <name>A0A8S1SLW9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cilia- and flagella-associated protein 53</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0090093</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CFAP53 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SLW9"/>
    <dbReference type="OrthoDB" id="307306at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043596">
        <property type="entry name" value="CFAP53/TCHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043597">
        <property type="entry name" value="TPH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31183:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31183">
        <property type="entry name" value="TRICHOPLEIN KERATIN FILAMENT-BINDING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13868">
        <property type="entry name" value="TPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="2 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trichohyalin-plectin-homology" evidence="9">
        <location>
            <begin position="135"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="426"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="50"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="432"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8140476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="484" mass="59677" checksum="F033477A0512931B" modified="2022-10-12" version="1">MIQNSTRNRSEQLIFQRRQQEQYVGKLNTKLRQEHYEKTFAAWENKGKDIANLQYTKNRLQQIRSEAEANKNQRREKLAFLLNEEHEQYQQEIKAMVETPEQVKERMMKEVAELKQRKEADRARQAEAAYNRRFRENADELRMVTQQFNELQAVAYRNMQMMEKQKMLEDQYEEEMIYAELYRREILKKERLEKEKELEQKAKVDERNKVLAVQTKMNEVKHQKTKEEIEQEKQMLKEEWKREEERHKQRENQFMNYKKEINSEIALNNEQQKEYKKQLKHQEKIEDKEMISQVLAREEAIAKMEQAEKQRQKEETRQFLLNFKNRTNEYSVNDQLKEKLINEENNRQWETKEAKWKAEDDARVKLMYEVYAQRADNVEIKKKLIEDEKNVKQMDKLELQRQMELYQKELEEKQRLEQEKIMQTKHNLINQMDEKKQRQQLLRDKKQQEEEALRKQKEEYDKKIELEKAKGRALLDELRKQRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SM95</accession>
    <name>A0A8S1SM95_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0080187</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SM95"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001744">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="39"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="322"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="429"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="322"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="344"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8139614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="621" mass="71943" checksum="086EF0FE1CC135F1" modified="2022-10-12" version="1">MRRPRFLSPQERVKSPLAHVNLQIPRKQSLLMQTGPYVFDSSQVIGKGQYSVVYKGHEQNKPNQIVAVKVITIQPNNPIQMYMIENEINILKLLDSPHILKLLYHNQTHKQVFLVTEFYNKGDLLQKLQRSLISEDEALQYTLQIAKALETIHQHNIMHGDIKSANILLSDKCVLADFGFAQKIDQAQQTFIIGTPLYMAPEVLFDNQINCQADMWSLGCVLYEMVFGQSPFYSENNDILKITLSKFKEHGKLTFPNTGVSKKIQALILHLIKIDPNDRLTAQETVGLLEPRSVDEQLKLNENQIKIKQELLNLDEKDTQIQVPQQQQTNINDSNANSNSQSNQTPIQDIQSTPDNQKNVGDNNSQDEHRRSTKNSNSSAKRKKLLQQLNNINQRKVNSYINNTPSQQTQQQNTQNSDVKKVLHKNNQNPQQINVNNNNNNNNNNNNNNNNNNNNNNNNNNSSSNKTISLLDLVNYFYKVYQLILSGKELKLAFIYLKLVNIYYMAYLEEQSKQQKKVTFLSCQNDYFELEREIKNKYPHWFKEEEFQKIFHSKFVLTQDFLKYSLNYLNKAAVDQQLDEKITEKIKKTLQIISILVSNDKNSTIQLDQQIIRLHRLNSKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SPM3</accession>
    <name>A0A8S1SPM3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">60S ribosomal protein L6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0110183</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SPM3"/>
    <dbReference type="OrthoDB" id="2436667at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13156">
        <property type="entry name" value="KOW_RPL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000014">
        <property type="entry name" value="60S ribosomal protein L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000915">
        <property type="entry name" value="60S_ribosomal_eL6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041997">
        <property type="entry name" value="Ribosomal_eL6_KOW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10715">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10715:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01159">
        <property type="entry name" value="Ribosomal_L6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="121"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8142483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="21298" checksum="67F63B61B8D85E20" modified="2022-10-12" version="1">MPAKKRTVRIGNWYKSDDENVHFVRKRKAPRPAKLRKIVPGQVLILLAGRFQGKRVVFLKQLKSGLLLVTGPFKINGVPLKRVNSAYVIPTSTKVDVNGVNAGQVDDDYFKRTQAQRRKNEQGFWAKRGELTSEQQTAEKTRLEGKRKTQKSIDDALIAAVKKTALLKQYLGARFSIGKTTRPHELVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SVN8</accession>
    <name>A0A8S1SVN8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Palmitoyltransferase</fullName>
            <ecNumber evidence="10">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0120155</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="10"/>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SVN8"/>
    <dbReference type="OrthoDB" id="294591at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF43">
        <property type="entry name" value="PALMITOYLTRANSFERASE APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="183"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="228"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="11">
        <location>
            <begin position="137"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8143329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="363" mass="42884" checksum="886B881FEB4C455B" modified="2022-10-12" version="1">MQKQKYKDSKSSKHLYQIWQTENKILCNGKLLTGSENHKLLASVSFITIPSFLFYIFMSPEFAKNGQNGYTVVFVLIQITIYVFLWFTVCMDPGIIPKIRPEYEMNEELLEVPQKYSKVDYRFIMDSKMFIIKAHQFKLKYCSTCAIYRPARASHCPSCDNCVVRFDHHCPWIGQCIGRRNYIYFYFFIMSVSFKLIFVFGVCLSYIVDESKKRSSNMEISDAISETLAYNPVSIILVIYSFGFSCFVVGLWLFHTYLVFTNMTTNEYLKKHWIVESKNPFKRQNILKNIVNVLTCITQLKFLELKQFVYQPKEYMQSPINTQNHLNEVNDSSKYREEDKIQVEYRGKQMQQQNIQIISEIQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T946</accession>
    <name>A0A8S1T946_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0170191</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T946"/>
    <dbReference type="OrthoDB" id="306997at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="340"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="537"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="66"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="187"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="549"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8147886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="626" mass="72784" checksum="B2E1B6AEB090A9DF" modified="2022-10-12" version="1">MQPSYKQRVPCNLQCIQQEIIMENEIPELDTFEEFCSNIPDQDNQDINFSKQSQTNNSQLIRIDCQQTHESRSSELQQSIDSLEQKSKSKIKSVQSQENEQITYMQFIEFNPQSIISESTNQRVDTYQSQKDTMAEPPAPQNKNTTKFCFPSKGSGLNNLKKQLCVNQDIQNNNKNQSNSEQTVLKNQSIQENKNNIEEDLRKQVTQINKEKTQMQLEYQQQIKQLMNQNQYLEQQLIEQKQNTQTLNSDKLILQSKIDILSKRYSVDKNTKLETCNFMESMPIKMSNNEKIQYLIEKLNQKCQQNARLQYEIILLQNNIELLETSKILQLTNNNIFTDKINHQNHSPQPQSENRAQSFSKDSRTSLLTLQKQPLNITTNYPIEGKNIVNLSAMISPLSKCAQIIQQKQLNQKIQKNAQQFQEDIQIQKIHTKNSTSVGELMSMKSDTINYSNIINRILRKESYSISSPDGRATQKFKQALNDKLNNLQQDSSKSYGSLLFSLGEQRLKDANSCFRQLSPKRDKKKSKQQLTEIQTRLFGFMNQQNSRKQSDLLKSEESHRSDLGRRSEKKNGMDPNTEIIQIMKEIQHKKSDISKNIKQLNPSTLITRPMITDIKEAQSNRQQKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T967</accession>
    <name>A0A8S1T967_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Alpha-type protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0190323</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T967"/>
    <dbReference type="OrthoDB" id="422053at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04515">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004166">
        <property type="entry name" value="a-kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052969">
        <property type="entry name" value="Thr-specific_kinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47763">
        <property type="entry name" value="ALPHA-PROTEIN KINASE VWKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47763:SF1">
        <property type="entry name" value="DUF659 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02816">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00811">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51158">
        <property type="entry name" value="ALPHA_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="VWFA" evidence="5">
        <location>
            <begin position="280"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-type protein kinase" evidence="6">
        <location>
            <begin position="539"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="7"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="144"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8149815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="783" mass="92166" checksum="E8C4253C3B3EAE29" modified="2022-10-12" version="1">MQNEGYSTNLEKAITILQNDKKYLQNENDKLKIQLEESIEEVCLLKAETISINDLLNKEIEKNKINQIHIEQLKNQPQLQNEDTKFENLNKYKNQQEKLCSLEQKILRNCEEMKMDKQRELDVEFHKFLDKLENKFNVDKQKYFEKYQLEKENLKDEIKKLYQEIIQYRREIEKLKNQLDKQQMDGKNSQKFNESKILDQRLEIYNNFEKEVPTLKKNQDNLEQRNLELKNQEQSRQNRLNGFMEIREQQQFRQQQENEQNKNTIKQMFRHIKKSHGFIEICFLLDITGSMDPYKQQAKKCIQESIKQIKMFTQKEAKWSVIGYQDFSEYKQLGSQYRQLKFTQDSRLVEEFLDSLVCAGGGDAAEDLRGGMKQMVKNLEWEKEFRLAILICDSPTHGRKYNGGVSDRYPNEDIEDAILELIDKNIFLMAIKFNQVTEVMYDEIKKIYQAQGKPELLLVEDAQNIGISNLSNLFVNLISNATVTITQMNNTASKINNKKTFQKIEIISAMENLCKKNTDPVNFDQDPEVEVIETKFEVFKVELSDQFLENHIQNIKAFKTENCFKPIKEPGLWDCKRTVHPFASGSLKQVFLMKKKDTPTELYAIKMPLGDKTYQSIEDAVKDCRSHLIAKGLLEKFIKDLEKNQKEDTELQIFDVQYTNILILKDVKQKNSFWVAERYFSGKFVKYNNNSAFILDDSNSLNHLMQAFSFYTFYKSNLNYIICDVQGIGQYMTDPAINTKEGNFDETDIGETGIKSYLGSYQGKSHLGRDYLRNLNIPDFEDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TGW9</accession>
    <name>A0A8S1TGW9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0210145</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TGW9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8151047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="65" mass="8039" checksum="B379127DB1D8FA8D" modified="2022-10-12" version="1">MRCHKCFFTIAKFNHLKDYYAILNISQKIDIKSLIILLLRNFFEILIKQKKKSLRKLMKRMKFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TM39</accession>
    <name>A0A8S1TM39_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0220278</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the metallo-beta-lactamase superfamily. cNMP phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8152099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TM39"/>
    <dbReference type="OrthoDB" id="421226at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000312">
        <property type="entry name" value="cGMP-dependent 3',5'-cGMP phosphodiesterase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000029">
        <property type="entry name" value="Cyclic nucleotide-binding domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000283">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23023">
        <property type="entry name" value="Anti-Pycsar_Apyc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="10">
        <location>
            <begin position="616"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="10">
        <location>
            <begin position="752"/>
            <end position="848"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8152099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="869" mass="98853" checksum="6EE1D4FFA9DB8BD3" modified="2022-10-12" version="1">MACSYVFSDDKSFLQDLDYMLEQSDQCPSLLKSQSCSIQLYKQQSDCKSNVSCADLVDLERGGCILQTKIGPIQYGMPPETIKDCLNLGMEVPTFYIIPSKRFDKRLGISTAEFEFPAYFNFFCKKKQITLICTPDTEKAIRTVFQETLLGPIDHPDLALDFYHKFPKSAYPDFHKERSVFSKNPSNLDEKLTIDHLLKFCLFDENNTATITEGDITIKIVKDNNSFKILENGIYQKSIQDVFTFPSQYYEYIVTKPDDYRAESMKNIKSKRDAAKEQCPTPLLVKQLPEMTVEQKVENWVIGNQYQRGGEKKLSVVLWDQMGGHVESGSAIFQLDIDENKSFTPPDFGVTILGCSHGFDPKGSTSGYIFWVNGRGIMVDPPPFASYHMKQMGIPSILICAVIISHCHADHDAGTFHKILDDIRVEIITTRTIMNSFLRKYSAMSNIDIDSLRKLFIFRPAIIGIPLNIYGAYFNFFYAMHTIPSLGFSVKLENKSIYFSSDTFFDPDQLLVYKNQGILSQKRYEHLAFVEFKEDLILHEAGVPPIHTAQSVLGKLPNNIKNRLYLVHTAQKDLKKELGLKIAKTGIENTMILIPSNTNKHLTTIRRLDLLSTIDIFEHLTLKNVRWLLDSVTSEEFFPGQDVIKEGTAGDRFYIIESGLARVYSTQKGQEFERYYQVGDYFGESAVLSNEGRRGASVEAVTHLKVLILEKHDFWFIFGDGLGGQGPIIQKMKQLTAARRSKAVHCLFKNSILSELTPSQKTQLEMIMREHEIEKGTVLWKVGDKPEFAFIIKRGTFAFYDSPEQDLEELDSGAYVGELKAMIENTAISTSIRATRKATIFKISKKDLISFANKHPGIYMILIDTKYVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TTL2</accession>
    <name>A0A8S1TTL2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sfi1 spindle body domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0300013</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000030">
        <property type="protein sequence ID" value="CAD8157161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TTL2"/>
    <dbReference type="OrthoDB" id="301267at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="388"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8157161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="688" mass="83915" checksum="8ADB6BDBE34AD439" modified="2022-10-12" version="1">MIKVESDVTPNFQPQRDILKQMEETMMVLQRPKLLHAPLIQQEEFSQQSMLLKEIDFFCQRLTTVKKTKPKVEDVSTKQFTCSTPEIQKLQMLIEPDEMDEQQHQVKFADEQQQDLEKIRTIPTEQGYQKRTGIVRQQQPKLIIKKLEFELENQPSWIPVKIEKKLEQELKINLNLCIFWPEEELANLRTQNINILSIIENMKNINYFNQSIKQQNNNNCQDFTILSIQLLQQLKIFSIILCLNYIHPIRELCIRANEIKRELLSEALYERKKNMLLLEQKRITKKLFLRYMFLRVKYKSAIQKILNDTIQIRQMINKKKCFQKLYLYSQRNKSKNEFYELVCDHMRVQTLNRIFQNWRIFQQFLVRQRITAKSIKNIILRQSLHSWQQNTQQIIQQEKRKLQIEKEQREKAQIVENQKENDEDVDDNQVLSQFLTDEKPRDMYEGPYCLNDIYTIDEREVQARKIKVIIFELKEDLKLCPNLMQKVFIKWKIAFLQRHRQKEFIQIMQLRKMQKVFKFWKNDKEYHPFYQFLLKRKGLEGFRYNQLTNKQQKLQDQLKIITLKQQRQQIEDMAIEIYNKNVQKKAFYAWKYQLKNENLHILWQSLSDQKLKQDFQIVQLKYDIIQKKRKIKYFKKWVLNFIKSRRSIKNLQQCEVTAKEKAIESLIRIAFETSMKTQFKWLEYYKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TUA7</accession>
    <name>A0A8S1TUA7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0300123</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000030">
        <property type="protein sequence ID" value="CAD8157381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TUA7"/>
    <dbReference type="OrthoDB" id="16692at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016314">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14497">
        <property type="entry name" value="PTP_PTEN-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051281">
        <property type="entry name" value="Dual-spec_lipid-protein_phosph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014020">
        <property type="entry name" value="Tensin_C2-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029023">
        <property type="entry name" value="Tensin_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305">
        <property type="entry name" value="PHOSPHATASE WITH HOMOLOGY TO TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305:SF60">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3,4,5-TRISPHOSPHATE 3-PHOSPHATASE TPTE2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10409">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01326">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51182">
        <property type="entry name" value="C2_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51181">
        <property type="entry name" value="PPASE_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatase tensin-type" evidence="5">
        <location>
            <begin position="13"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="4">
        <location>
            <begin position="97"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="C2 tensin-type" evidence="6">
        <location>
            <begin position="188"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8157381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="361" mass="42329" checksum="DE629313D759AA16" modified="2022-10-12" version="1">MDYIRTIVSGKKKRFISEGYNLDLTYVCDRIIAMAFPADGFESCYRNPIDKVVQFLENRHGNNYLIMNLSSRTYDYSKFKNQVKNYQWNNHHAPQLHLLFNMCKSMQEFFNQKQENVVVVHCLAGKGRTGTLICCYLLYCGMFNSVNDVLQYYEKSRFNDEGLGVNQPCQIRYIEYFNQLLQGESIYPSLKYLSSIIIEGVPKLNIDDGSKISAKIYQNQQLIKDTQILKIIQENQTNCFIMSPNPIVIHGDILIEFYNSNLVKTKLIMRISFNTSFNTITFTKDQIDPFKIKNDQRIPEKFKIHVCLSEYCNNCDQTTSFHDKCPDCKQTLNQERQNWHQIKEAIKLKGVNIMRNFPQNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TWZ2</accession>
    <name>A0A8S1TWZ2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0290105</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TWZ2"/>
    <dbReference type="OrthoDB" id="286207at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8156660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="194" mass="22253" checksum="267F49141F91F843" modified="2022-10-12" version="1">MSDQLNFNLKLYRERLTDKYSRNNSNSIIGQDNLLSSQRKSFKKNAPTLDSISSRLQNHSSNKLKTDSRVCSPKNLSSPVTLNGMQQKIKSITTSPQQFVNNNFGHPISSPKSVVTHFQGFYQSPRAQTDRNEIQQIKRYNSNNDITEMIPFHDLVNMRNKLENYDINAAHISPTYLSEIVKLAHVINTQLQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U1C6</accession>
    <name>A0A8S1U1C6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0310211</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U1C6"/>
    <dbReference type="OrthoDB" id="305778at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="451"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="333"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="454"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8158184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="489" mass="57872" checksum="16B0209551D60F77" modified="2022-10-12" version="1">MSQLKNQKNSPGYQNNHLQQGPNQPQIISQQSKVLSDESPFNVSKILKAKQQQSNIFNLPQKDQIDIQALANQNAEKLLPYDIFWWNSQNVKANQKQDQLSYQIPNSQIIQSRPQTSPQQRFELEQVPNYSELTEEQFLQMQQQQYQLFLQQHQQQQKHQSKSQEQHNDKQKHQNLNQDSKPNAYNLMNENNQMIQTASFNEKQRQIEPKFQENEIKNQQKQEKQQFKKEQKKQILNQLQNPEKLYNKINNPNIELKQRNPNFSDVLGNGLEKYYQQKPQQTTIKRSLQDQLQESELYGVNYHQRQLLKGMSSQMDTHKYYDQMLDKQNQNQIKLQQPKSTSKSPQGSPGKILEKDDTLFTKSYYTELTIRGMGKEINWEKLTNQLNIHQMNIADIDVVKNKQGQIKQHKIIIKHLDSVNLQNVKQWVITQGGRIVEEKQIKQDEFQRLQQIKDGSKQRPLKISEEKEKLKQQQKKRPASAIPNQKQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U1S6</accession>
    <name>A0A8S1U1S6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0310361</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U1S6"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002141">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="10"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="484"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="510"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="552"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="594"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="636"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="678"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="720"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8158478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="839" mass="94396" checksum="8AA0F3E0C8D22B2B" modified="2022-10-12" version="1">MLIKVEQIEVKKGDLIGTGGNGQVFQGQRSDGSFVAIKQQMTISDHEQKILQAIRKKNKDGIFTIMELAKSLDLRQIQDKRKACLQMAKGVFELEQLGFFHRDLKPDNFVMGEDGKIKLIDFGTTKQNQKQTNTAATGTYLYMAPEMIISTNYDSSVDIWSLGVIFYEVFTNETFFLICDTSFKLQLAITQITQFQINQKLRKKQINYQLNELLQKMIVQYLDGEQKIIILKKRIRIDEVINELEKQIEVEQTQQQIKILEYKEDLSKILTSSNYIDECILNVIIEILRKAKISDCLKFLSQNKSQTLIKQYILQIKNNITLDKQQIIGDLDEKIQKITNLLKQIKVHDFNKISYSIENQEKTKQDLLNKISHNEKIIKLLKFLIRLTAFDNLFIYSGSNSLHLLVDMKVDIRNQSFENIRIKNTSVIGGNFVRCNFSGSDFDNVDISGLNLNGAQLFNCKWKNIKVHELNKLDGHSSSVRSVGNTLASGSSDQSILIWDVKTGQQKAELDGHSNDVNSVCFSPDGNTLASGSVDKSIRLWDVKTGQQRAELDGHLSYVYSVCFSPDGNTLASGSVDMSIRLWDVKTGQQRAELDGHSNRVWSVCFSPDGNTLASGSGDNSIRIWDVKTGQQKAELDGHSGTVVSVCFSPVGNTLASGSIDNSIRLWDVKTGQQKAELDGHSNDVNSVCFSPDGNTLASGSSDQSIRLWDVKTGQQKAKLDGHTSYVYSVCFSPDGNTLASGSVDKSIRLWDVKTGQEIKSSDKNYKDILAKFKAPLFQNNPLPVSNYPILVISQELILEAQDTLILQGEFMNHQGKDLKPLFKFKGSCFLEDLKQKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U8R6</accession>
    <name>A0A8S1U8R6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dynein light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0360268</name>
        <name evidence="3" type="ORF">PPENT_87.1.T0490249</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U8R6"/>
    <dbReference type="OrthoDB" id="10260741at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21459">
        <property type="entry name" value="DLC-like_TCTEX1D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.40">
        <property type="entry name" value="Tctex-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005334">
        <property type="entry name" value="Tctex-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038586">
        <property type="entry name" value="Tctex-1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21255:SF7">
        <property type="entry name" value="DYNEIN LIGHT CHAIN TCTEX-TYPE PROTEIN 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21255">
        <property type="entry name" value="T-COMPLEX-ASSOCIATED-TESTIS-EXPRESSED 1/ DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03645">
        <property type="entry name" value="Tctex-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8161601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8168704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="125" mass="14772" checksum="EC417E72F4B9D6E8" modified="2022-10-12" version="1">MLKKPQQQNQEYQIKPKPREKFKSSKCRELIQEILQQKLKTGSYQDVTTQSKDIAENIKNRLKQQYTVTSRYKFIVHVLIGQQRGQGVRVGSKCFWDYDTDMCVSETFINDSLFCLVTVYAVYLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U9H7</accession>
    <name>A0A8S1U9H7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0350024</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U9H7"/>
    <dbReference type="OrthoDB" id="294916at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8160513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="579" mass="68219" checksum="152A7849D623F18B" modified="2022-10-12" version="1">MIKQLFKLGTNVCGQRIGQNFKLFNYYQRFYFTTGTQTIDQLKNKLTELNSKFYEIEEYKQKEKIMLDVIDVKKQLNTLLFPNKQEENKFYEGYRRDLLVLAQLNFENDKREECSKLLNEIMQGDFRNEFKDHKDENQAFLTGLFMIHCMSNLDKLSIAEQSAGEAVQYLQYFIDNQCPIYQASIQNQTPNTENLFEQELQHIQNMVTHLSLYSKICQERGKFNESIEQLGIAATLLDNTGHLFRKIFSKQITIKTELKEDDLKRHFMNILINEAIILKNLAKSCLQLGRFEDGKQLIDAALTYFDYLIEQKLLSPNDENYVATLIEQFNYLYQTNGNVEDMKTIKNKLDDIAKNTKENENTLYSLIRYELFLNKYFDTDIQKLNDLMSKIQQDVPMQLEFNQQRIQALLAKGDHKNALEQCKAQLNILKSYFDSSHQLYGETLVTLLELAINEQNYLSAKDYIVQLSSESYNITYISDIEQIIKFQRLRTEFLERFNQKESTEQLRKILDKIESIAFTKFSNSYKKSSLLAEFYKFIGDTNLQKEKLQECLNVLQQNVTFAKRDELIKSIKDTISKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UBB7</accession>
    <name>A0A8S1UBB7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0370219</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8162138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UBB7"/>
    <dbReference type="OrthoDB" id="7869584at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="10"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8162138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="491" mass="57629" checksum="959DF89E022093B7" modified="2022-10-12" version="1">MRNEYEQYYETKNQLLGQGAFSQVFLGVKKSNQQQVAVKISNRNVAICQQEKMILLQLSQFKHQNVIQLFDIFDQPEALYIVQEYCPGGTLYEMMITKSFTEEQIVNIALQIASGLNFLHSKNIVHRDIKPENILRYVDNQGFEIYKITNFGLSSVKLERMTTTKVGTAHYVAPEILSKQQYDKSVDIWALGLIIDELIHKTPFYNGDTEEEVFGKIKSINYIIRDKQYAKPAILDNKKNLIKNILINSIQKDPKLRKDLNWIIQTLNEYQQQTPHSSYQKNNRNLSLNSQTTSCEQTQISQIPVYSVDLQQEDECPYHLQPIKYKIPGGDDNIITNACQKCLQSYGIFYYSEFQQYLRQTNVELVSFSIELYFSQNEIIIEALKLLNQFKFMQEKENLISELRNIVVKHNQILRLEYIQYQDDIQSMNVNAKWINDALKINQKISKDMPNKIIQYLISRCQERKQISLQECRQFIKEQLKLLQQYLVSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UBN3</accession>
    <name>A0A8S1UBN3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0370281</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8162260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UBN3"/>
    <dbReference type="OrthoDB" id="288891at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8162260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="313" mass="37169" checksum="FE93ED28C624BEA7" modified="2022-10-12" version="1">MWRFTKSQIADFVHYRQQQLPEGYTQNEEMNIINHYCCNLLPTLFKNEKNSQLTLISYAAVLFKRYYMIKTIFEQSGMHQMLLFCSYLGMKLNGLSQQSIAIQAQQLKKKPKHERFHHYGDEFYAKYEILIVQALGYDIDVKPIYPIIQSAIFIYQIKDLLEQSLFIRYEKKIEEYFLKSLQGFTIFTFKESEIALAIVECSLQSIFSEILSPHISLGGIKPLKQLTIEKIKKYYQEYKQNGTLIIEQIDQRDISIANHSQQIQEVVHLLQEIKDISREFEKNYERYKQRKQVVFSLPMSVEFLDVKKQVAYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UBN4</accession>
    <name>A0A8S1UBN4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Kelch motif family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0380171</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UBN4"/>
    <dbReference type="OrthoDB" id="191037at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19756">
        <property type="entry name" value="Bbox2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF3">
        <property type="entry name" value="KELCH-LIKE PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="3"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="9">
        <location>
            <begin position="76"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="144"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="227"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8162651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="619" mass="71418" checksum="5608F504B056A345" modified="2022-10-12" version="1">MECPMCWELYAQNRVARNLLCGHTYCSICLESIYNVNKRIECPLCRTKHEPHVKPHLLSKNYVAMDLASKHLEVQKKFELCPIHIKLPLQFFCEDDQSNMCTECIAEHYGHKFFKYEHSGRIQTKLKSQFDILEKQVKGFDNCKEQLQLENVKLMQQLDEQFDRLIKKIELRKQELKDQQLKIFTTECEQIDQELAFNQSLLTNMTSLTTKLEQKQGELKGVKALKNNDLIKEIDDLEEQVDRDVAQQKQLKISEIKKLPKLVFDLKLINEVSKFGQFKKDVSNPQICFFGDKHKILIYNIENNEWTYKQMPNNTLEYNYYAAAVSLPSGDIIITGGGVSRNTMLISPSKGFQSQALKSMYFPRKEHACVYLDGFVYAIGGYDGSAKQMLSCCEKYSLVSDEWKIIDPLQKQKCAFAAAAAINKFIYVFGGFDGRERLNTIERYSVKENQWKVLDVKFKQGFSNAAAISYDDNKILILGGGSNQGFSYDLQVYDVNEQVVKTLSRMNEGRDLRNKLIIFDNNLFACGGNNQSIEKYSLSQQVWVNLKSYDQLVQDNLDSWCSALTFEINSSTSVSNLLKHNQQKVSFDNDSFNQDAQSEELEDLEDYAYNQQVGGLFQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UCR9</accession>
    <name>A0A8S1UCR9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0340320</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8160406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UCR9"/>
    <dbReference type="OrthoDB" id="1664597at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19502">
        <property type="entry name" value="RecA-like_PAN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000005">
        <property type="entry name" value="26S protease regulatory subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000440">
        <property type="entry name" value="26S protease regulatory subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000027">
        <property type="entry name" value="26S protease regulatory subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048723">
        <property type="entry name" value="OB_PRS7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21236">
        <property type="entry name" value="OB_PRS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="195"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8160406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="418" mass="46861" checksum="CDDF4C7EF7E86376" modified="2022-10-12" version="1">MTEEKETKTVPLDENDINLLKKYGMGPYAEKIKQLEEENKNSVTTINKMVGIKESDTGLSIPSTWDLVADQKLASEHPLIVARCTKIFKSSLQQKDLYMITIKHIAKYVAGLGEKVAPTDIEEGMRVGVERQKYAIQLPLPPKIDPSVTMMTVEDKPDVTYNDIGGCKEQLKKLREVVEMPLLNPEKFITLGIDPPKGVLMYGPPGTGKTLTARAVANRTEACFIRVIGSELVQKYVGEGARMVRELFQMARTKKACIIFFDEIDAIGGARHDDGNDNDVQRTMLEIVNQLDGFDSRGNIKVLMATNRPDTLDPALLRPGRLDRKVEFSLPDLEGRAGIFKIHARTMSMEKNIRYELLARLCPNTTGADIRSVCTEAGMFAIRARRKAISEKDLLEAIEKVIKGYQKFSATQKYMVYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UD37</accession>
    <name>A0A8S1UD37_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0370076</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8161883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UD37"/>
    <dbReference type="OrthoDB" id="285059at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000586">
        <property type="entry name" value="Rab family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8161883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="253" mass="29248" checksum="9BCECDFE36307511" modified="2022-10-12" version="1">MLQNNIDENEASLLFKFILIGDIAVGKTSLLKQFVEKRFCDDYNLTIGVEFNIKYIQVNDHVIKLQMWDTSGQESFKSVTRLYYRAAAGAIMVFDITRRETFENIRNWHREALDNGNSRMKFLLIGNKTDLQNHREVESYEAAQYAKENECLYTECSALQGSNVENAFMELAHQIYQMAMEDPNLVNEQYGIKYVNNANSSMSTSKIVSQQAIPKKQLQKINNSNTEEFMEYYGEQNQTNQKKAQCNNCCADI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UGJ7</accession>
    <name>A0A8S1UGJ7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosome assembly factor mrt4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0390239</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1 6">Component of the ribosome assembly machinery. Nuclear paralog of the ribosomal protein P0, it binds pre-60S subunits at an early stage of assembly in the nucleolus, and is replaced by P0 in cytoplasmic pre-60S subunits and mature 80S ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 6">Associates with the pre-60S ribosomal particle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UGJ7"/>
    <dbReference type="OrthoDB" id="10262308at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05796">
        <property type="entry name" value="Ribosomal_P0_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1730:FF:000005">
        <property type="entry name" value="Ribosome assembly factor mrt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.105.20:FF:000003">
        <property type="entry name" value="Ribosome assembly factor mrt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033867">
        <property type="entry name" value="Mrt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051742">
        <property type="entry name" value="Ribosome_Assembly_uL10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45841:SF1">
        <property type="entry name" value="MRNA TURNOVER PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45841">
        <property type="entry name" value="MRNA TURNOVER PROTEIN 4 MRTO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL10-like insertion" evidence="7">
        <location>
            <begin position="126"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8163474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="220" mass="25297" checksum="8EE47498AFC42F51" modified="2022-10-12" version="1">MPITKRKKTQVLTKTKKKTPEKKELLVKKLKQSLKKYQRAIIFQYKNLTTNPLKEIQQAWKSDSKLFIGKNKVMQVGLGRNEEQSATKNSYLLSPFLKGETGLLLTNKTLQEIQEYCDTYKIPEFARAGHISDQTIVLKEGIDTLKGFGHSIEPYFRKLGLNTQLINQQIVLNEKFILAQEGKPLTVEQSKLLRLMNQKLAYLEIAPLCVLEKNGTFKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1URJ9</accession>
    <name>A0A8S1URJ9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0460072</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the nucleosome assembly protein (NAP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8166797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1URJ9"/>
    <dbReference type="OrthoDB" id="27325at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002164">
        <property type="entry name" value="NAP_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11875">
        <property type="entry name" value="TESTIS-SPECIFIC Y-ENCODED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00956">
        <property type="entry name" value="NAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="824"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="44"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="173"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8166797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="845" mass="100417" checksum="1FDB02EF698CCE2E" modified="2022-10-12" version="1">MQRPLTSITSSQQQSKLYDNSSTSFRIKENKKNRQMPQQMLYDKEGLYEENLKLKKELQQVKFELKKERQEHANFEKQVQKTNETQKDFQVILSQRARIKEQDKLIKELEDQVFQLKRNPERTKNKELEAELEEQKEAYQKLQLYISSKCNQDANETVMINQSLKDQIQINLVQAYKQDNLNLSQMILSLQQENHGLQQNLLQIKNDKDRLTNRLKELEKQFDSVNKHMMYMRQEMQNTKKQTPSEDYLKKIDEQQQQVQNQAKQLQQNKKLIEELEQKNGELQHNYDVFKSMENKEKEKLQDRINMLKKQIEELNQKRDDQEQSQEGSLQSIGLKQAMTIYGSPRSNNSPQKLQLQMGFTNKKQCLKVNSYDIKEIISNLKIKLLSNNIDYETQEQILCEDDDEFLTLEETQFRLQKAPFELNEDESSLLARYLIEDNTEDHILYDKQRTQTKIIIKSVYKKLLGEFNLFDELEKQQMYEYFCQLFHKYNQGMESAIKQFNMKKKYLGNNQCEYEDFNEALKYCDILLTSRQRLYLELEIFSDSQQLQRFNFQTLFSKFKLKVPDLDNKAIKQLPPKQQTTAIAIQHHLNKWADSEKQVSKKVRQLQTEYLRSVIPLQNEINAIIKGLRAPTEDEIKDANTYQIEIKDVQASQLQNYWGQVLMNNSLTGKLISEADREIFRHLLSLNVELGENGKDFTLKFEFEDNDHFDACILTKSYIFEDSEDELPSKQKGSQIKWKEGKNITKKIVKRKQKNKKTGQTRDIEKEEKAPTFFHFFEEVDQINNEDEQIERQQFDYELGSQFVNQIIPKALFTYLGLKVDDEDDDFEGDEDESEGEEESLEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UU03</accession>
    <name>A0A8S1UU03_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Myb-like DNA-binding domain containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0490192</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UU03"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF69">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_38, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="55"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="59"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="103"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="103"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="2">
        <location>
            <begin position="106"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="154"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="162"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8168590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="26538" checksum="ABEBD77853CEAD31" modified="2022-10-12" version="1">MFINPVNSMFFCLPYFYSFQAPQTLVKEEASDIEKEQTVQNIQEQTNASGIVRGKGQLWSKDEVDNLIKYYKQYHGDWKKIIKHLKGRNISQCSQKFRKLQDQDKRTKRKWSTEEDKILLESFEQYGRQWIKIAEKLPGRTSKQVRDRYVNQINPIINHKEWNEEEDKIIMKEFQQNGPHWAQIAKQLDNRSENQVKNRFYYTILKKYNGELHPYLKIQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V0C4</accession>
    <name>A0A8S1V0C4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0520078</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000052">
        <property type="protein sequence ID" value="CAD8169977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V0C4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8169977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="128" mass="16062" checksum="A56247485FEA39A0" modified="2022-10-12" version="1">MLELMHQYKLQQYHKINKKLYQIKPNFPLNLLVIYFQNQLLISINHPLSKNKKQIKLPIKLIQLFPTGKWLFIQLHSYQKIQYRKWIIEFLVILEKRHLQKTFIIQNLERQFNQEYQKRQWRYLLINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V1T6</accession>
    <name>A0A8S1V1T6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0510234</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000051">
        <property type="protein sequence ID" value="CAD8169759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="286304at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002470">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002551">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002940">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:003567">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:003589">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:003665">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055439">
        <property type="entry name" value="Beta-prop_EML_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050630">
        <property type="entry name" value="WD_repeat_EMAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720:SF57">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_51, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720">
        <property type="entry name" value="WD-40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23409">
        <property type="entry name" value="Beta-prop_EML"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="56"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2207"/>
            <end position="2241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1428"/>
            <end position="1455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1428"/>
            <end position="1441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8169759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2600" mass="299644" checksum="925B5DBA7A7B4F5D" modified="2022-10-12" version="1">MGQFLGHLQNAQPPQSNPLLNNSISKLREYHGSFQSVCDTFSIDLTEFEQIFGSNETMFQIWDTDNNGLINALELFSGLIIFAESNFEEKARFLFDLFDFNELNSLSLIDLDFMLLSCANATFKIMQINNEVNEEEISDFLSNFFSDNQRVNISQFLKWCVKTEEIRQFLQLIKKEAPELKVTAQMEQLSQKIDVLRQVSNKEFRKKLLLPDSKRPYFDQRNLGSILSSKAYHSKIQWISSLAKKVYAPQPQVFQKDVYAKMNWVYGFRGKDVKRPLLYIKVQSNTDKALNEKMVFFTACIIIVYYPKINEQRHYLEHESEVISVAVANNLSLMASGEYAEQPAIHIWDNNTLHNIGVIKGVHQKGVHLLTFFGNDELLASCGIRAASPILIYNIKDFSLVLSTQVNEFAVDLLTIKNFVGSFGGIQQRQQQTSQQSQPNPFKKYENSFVVCTIQQIIQFQYYDGHFLTKEIYLEQYNLSSPLTCATALSIVAKEPNLKSYQDDSEEAIVIISGHQDGSVILWESFETMEQMTSYKDQIVCITSYQYGIIIGTDASTIHLWDFKFKNNIKNIDLTALSFKLFSYVISDIVVAGDKLLVSTTEGDVVEIFLQQKQEHQNNQFVNKLKANRINYIVQLSGTLQALCILERPDSDDKLVFCAGSQSTVYGFSLETHEIVDVWTIGDQISSMDCINFEDGGAVFALGTVSGKVYLRLDWEESPRWYDFKHQVNDLKFSSDTSCLVCAVQDAFVYVFFLNNTSYFQTAPKKIHFEGEFPICLDFVDDCKVFIVGTTMKNQYKIELPDMKSKLLSSENEKLNSTTWVLQYPLTSTSNQNSKKEQFLPILIGGDVKIFLAAGEGGYVYFWRDREQLETNCGGFLRGHASNVSRLQMTKTQDVFYTVGTNDNTLIEWKIDFINDLADFSKPFQQDDKQQINKPGLNNFSMASNKQDFNQTIDEIFKREKDYCFFLNNISDKFRDNFVQFRATNQKMLNGLLQELIPPFDKKQHVMKRAPPLSLTLDYIYGFLAYEKRRTLFYVHFYNKQEKKKRGGQQQQDLKSEQRKRMEQMNQQSIILPVQFQKEMLFAKQALLPYDDSHNDCQRHFVYITSRIAVVYNPLNNQQKFYEGHRFKITCLAIHPLKCFVATGESAPRPCIHVWNVFNTEPVKIIKTNHKNGIYDLVFSRDSLFIVSIGIDETYSIQVTSWKNETIIAFRNSGTFPICCVMFNPYNRYEFATCGYQNITIWSLQGRNLIRSQVILSDEVKYSNGCFITCLSYISYLLGDKIESDIIVGNNFGDLALVACGKYIVVKERAHQKMINCLKISEILGDKVVIITCGEDEYIKIWDTKFNLINEFNIRKTGFFQDGTPATRNLSAQSIDIFSCRVPKRHLNEEEVEESHNLSVMLVGTRNGDILEAAFQVEFQGLKLQQIKKQESHSDDEHSNSHDSSGSEQQQRQPYTTTETLKFNYSIYMRSHQSQILDNKQQIDFFSKKMFITLHPTQPIMVSMGEDQKLILWDTENNSLLFVKNMGMTPTAIRLSPDGDLLVIGFQNSIVVIMDSKIQKNANGKVSERYLLPTLDIIMNIKDKDNKTAVLNIEFSHKGDMLAISYDNARSQKDMFESKLEKEGSFISVWVNRGSHRTSKYRATDKNLFLKYTDIRCPQLNESYQNDNDTYGIAAYFMTFSQDGNYLLIYYQLINNQYTRINNDPQGVYVIWDLNSNTSVKNWETIKNIQWKKLNFPNSLHSQYQFYDSLIGDPKQGQEQETNLANEIFINPVMSVMVDISPFLICGSTNGDLHLVKSSCLFYDKDFITEISKKQKCLAKSYSAHVSFVNQIETHQNAQYLYTTGILDECIMKWKLTEEQQNWDLDNLIYDKKQADLFQEVQEKEKFKSLFSELLPLRKGISDKVKNVDDTKQPEVELKLQSIIGRRAFTRRNNLFYDYDERLIYVAGCNLVIASLDDDDEQEDKITSQFKKYNIGQQQSENNSVFQQFIKLDQSDTSSSPEISCITLSQDKKLLCAGTIELKAKLLIWDICSRTCVKNIVINNVGMIMNIKFAYDNRHLICNAITQEYRQEILLIDSENHQVLGVVTYSYSIPYKIKDLEFYPNSVFRFVTCGVQHMSCWQFAGGQLTFQAMEIENPKDLVELVDNQNDEQEGVQDNDQEDGLRITFLTVIFVQDAIITAGEDGFIYVWDDKKINKKQKAHPDQPIFCLYTSKDSSMFVSGGMDGRVILWSLSKSEYSYVVEKIYEYSIANEQLQKTGLNPNLHIQSVCIGQNYILAGTRSGDIYELVRPNESDLKSLTKIQKDMVKLRINCTDHDQPKVVAFSGNAQKLYSITQKGLFAVWNLRKLKRTYSFAFEKATLNLIVCKLSPKIFIAFEQEVIVLNDHDYSVNTNYSLKQKSAISDMKLSVDEKMLALALARNQEQNAKIEIYDVENEENNFRLLCSIDNLNTSVEYLDFSTDNFYLFYKDVLDETALIDLDQQKRINSMHMEFDLEWCSDGIKLAEKAKGVHSCYTDDNKIRKITLIGEKSMAVTDNMGTIRIFNYPCTSGQGYMRIYTDHMMYINQCVASPDKETLVTTSEQDKCIMVWKIQKVDVNNTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V3S8</accession>
    <name>A0A8S1V3S8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0540118</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8171113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V3S8"/>
    <dbReference type="OrthoDB" id="288761at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8171113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="183" mass="22030" checksum="B98DDC1261A537B5" modified="2022-10-12" version="1">MSNNQTFKDFPNQSHIELKFNTKDQHGLEREEALQKYRRNINLLTKLFAYDVDQPDQEKQNDICQSEKQNGQYQIKTHLLESMRLSLVQLQNRHKETIEQFKKRNELFKQRINEDTKINNQEEFQQFINELQSEDMLCDSHPFLDKKSSFYMQTNLQQPIQISQDQCLELKEQLKDDQCILLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V756</accession>
    <name>A0A8S1V756_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0560177</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000056">
        <property type="protein sequence ID" value="CAD8172223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V756"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8172223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="154" mass="18099" checksum="7A49D3332A42FA0E" modified="2022-10-12" version="1">MIKIYLFNVLNHVKFVQKMVKPVPLVQIQQTKQLLVVHEKKKIYHEHLNKLIIFKIVQKILKSVVEHPYVLNAKIIIGVMVVSVAHVQDPVQIAQEIVIVLQFFNYMQIENQKILQKQNYFQIIICLNILKMIVLALILYAKHLLNVNVQKVMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V7R3</accession>
    <name>A0A8S1V7R3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0550264</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V7R3"/>
    <dbReference type="OrthoDB" id="303010at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8171889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1003" mass="119043" checksum="EB51EA6FE49F4DBC" modified="2022-10-12" version="1">MFEEIKVSEILQSDIIEHSKITLSGSASNDIYQCTDSSISLEIDQTAYILKLANSQTVSINIYHMKEIYTKPISYTCEYVNFTCPITQNGCTLINDNNTPYLLVVTQVGHIYYRNLKTNTSMLLKNLMIENIKIQCFVKSLIQKEKFEFYLMTNTDLLYHVTLDVVGGEFQKNKYQISFGTKIFSKLLNSFSQDQKIAFQLNDKQILNFSQNYQFSLLEMQKTEFQTIQLQCQDRIKSNQIDIFQQKPSGKLFVCYQLNDNLKICTINQNQLIINYVVNLSKIQLNNHYYLSSTQNQIILIQDFEIYQLDQQNMNLELINENQYSVYGYLSQDDKLLLFYKNQLTYIRLISDKISIKQNELRNQVTKFKNNLKKQQNMIDDEIIIQSIAFHPTSDNILNQFQQLIKIMHRNLDQRKVISIMKQQYNNFLPVQFIQNLMDEVEDIYIYIKPTFQKLQLDINVIQNELIYQLETVNSNLQQFFKNNDQMNLLNKEFERSTNKQLIVKSLVIHTLRDYYQMQLEIFLGLGVLQSLNQQSIQFDIKFKQFYDTMCLLSLVEDEKLIHIQNILRNQFHRVEDLINFSNLNSIQEVLKIYINLIYNEIFQSFTIPDEYFCEEFIHNQMIFHSHHINSKGIIRHCRIAQQTNQIPTDVVKFIKENQNLNQELKLKISFVIQKQNPKQDEEQKIVLNCLLYYANELKQHHLLFQIINLNDQLQLKDSLKLIVDNLLSFRLDVRTTDKIGEYLLSFSRDKYQILSNKNRIISYFSFLIKMESKAKAYTLLFEYIVNLESLLFNISQKDLVFVIRTQLDCITLLLNQMQLHSRQDKKPYALQIVCKIKDEIYRQFLNPSTLGELREFKQNSQGNIRVINFEQIQLFNSYKQIQYFVSINYTPQAQLQKVVEQLILHNQIDQLIEMGKLRIDPYYERNVAFCYFWNENSRKELINKLTDVMKEQEFSIVLAQICVYCNLKSIHCQELYKIAPCKGSFLQYLISVDKEQIVQEII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V9J1</accession>
    <name>A0A8S1V9J1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">MsrB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPENT_87.1.T0600117</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Methionine-sulfoxide reductase that specifically reduces methionine (R)-sulfoxide back to methionine. While in many cases, methionine oxidation is the result of random oxidation following oxidative stress, methionine oxidation is also a post-translational modification that takes place on specific residue. Acts as a regulator of actin assembly by reducing methionine (R)-sulfoxide mediated by MICALs (MICAL1, MICAL2 or MICAL3) on actin, thereby promoting filament repolymerization. Plays a role in innate immunity by reducing oxidized actin, leading to actin repolymerization in macrophages.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-methionyl-[protein] + [thioredoxin]-disulfide + H2O = L-methionyl-(R)-S-oxide-[protein] + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:24164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12314"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>[thioredoxin]-disulfide + L-methionine + H2O = L-methionine (R)-S-oxide + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:21260"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58773"/>
            <dbReference type="EC" id="1.8.4.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000060">
        <property type="protein sequence ID" value="CAD8173890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V9J1"/>
    <dbReference type="OrthoDB" id="44061at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033745">
        <property type="term" value="F:L-methionine-(R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033743">
        <property type="term" value="F:peptide-methionine (R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030091">
        <property type="term" value="P:protein repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.20:FF:000008">
        <property type="entry name" value="methionine-R-sulfoxide reductase B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028427">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002579">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10173">
        <property type="entry name" value="METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10173:SF52">
        <property type="entry name" value="METHIONINE-R-SULFOXIDE REDUCTASE B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01641">
        <property type="entry name" value="SelR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51790">
        <property type="entry name" value="MSRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0712">Selenocysteine</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MsrB" evidence="16">
        <location>
            <begin position="13"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8173890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="167" mass="19684" checksum="72A7D1D096E08AD8" modified="2022-10-12" version="1">MLRLYYYTFSKLIKVDRLTMTPHQYWIAAGKGMERPYTGEYWFNQEVGTYHCKHCENQLFSFDTKYKSTTGYAQFWNHIPNSVKLEESNVKEVRDLCCAGCDSFVGKVSFDGPPPTFIKYSINSAALNFKLKPFQEDPYFRKIARKEKQAQRSKKVEHNYLESNKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VDR3</accession>
    <name>A0A8S1VDR3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0620101</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8174997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VDR3"/>
    <dbReference type="OrthoDB" id="306763at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48038">
        <property type="entry name" value="RIBONUCLEOPROTEIN RB97D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48038:SF1">
        <property type="entry name" value="RIBONUCLEOPROTEIN RB97D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="15"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="5">
        <location>
            <begin position="92"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="106"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="117"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="140"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="150"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8174997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="160" mass="18361" checksum="EFB635EED927598B" modified="2022-10-12" version="1">MSSSDSHQHKKEETNRIYVTGYSAKESEMDIKSAFARHGEIQEFSWKGRFCFIAYTKPEEASDAVRLMNQQEINGRSLIVELARAKKKDGACYQCGKQGHFARNCRLNRRSYSDSRRHSKKKKKQRKHRSSSSSSSSEKKKSKKRKRKSSSSSSHSSSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VGG0</accession>
    <name>A0A8S1VGG0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">4Fe-4S Mo/W bis-MGD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0640004</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8175771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="4405280at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002895">
        <property type="entry name" value="Paramecium_SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01508">
        <property type="entry name" value="Paramecium_SA"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00639">
        <property type="entry name" value="PSA"/>
        <property type="match status" value="24"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035828074" description="4Fe-4S Mo/W bis-MGD-type domain-containing protein" evidence="5">
        <location>
            <begin position="16"/>
            <end position="2531"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="6">
        <location>
            <begin position="2031"/>
            <end position="2104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8175771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2531" mass="271711" checksum="168ADD94D9FC227B" modified="2022-10-12" version="1">MKILIIICFLAMVLTQQVKHSNQCNDCGQLKSQNDCEQETTVTGACEWVAASGTTPAKCQKKTTVDPVTSFKPYCELIDKPETNCAKTFGCAYVDMKCTHFTGCQAYVQTTTTNCQAISYLCVSDGNTCIQAKECKEYTQQQCESTPSVSGILKCKWDTNSGACRDYACSEADVSLNTDEKCSAWLAGCVTKGQGCVNTPRPACATYTGDDVACQSFIGSDGNCELATGTTNCKAKECFNAPTSLSSDDDCKAYQKGCITTGKGCVLATTKPLCSTYSGDNTTCVGYIGSDGVCEGDAGGSKCRARKCENGTFNTDDLCKQYQSSCKTNGKTCVSSLTACNTYKGTATTCAVYIGTDGYCKGTSTTTEASCLPKVCDEAPDTTTTDDACNKYQIGCVTTGKGCVTKTNLKSCATYDGDATSCQSRVGSEGKCTWKTGTNCVARDCTQAATNLNTNPLCANYFTNCVTTGSGCVSQTTCDLTVKQQSCEGTNNCSWQPICTSNSNCSEFKKKSICLANQARVRTFDKNDENGNPLYIFVSKKCGWLNNSCKELSCSDLTGPYYNNDTNCAAELSTCISNRIDACITKYECSKLSGTQSTCLSYPGYCTNSSTATDTTPCIQRKCSDNTEATDNATCASFLPGCISNGKGCVDYTTPCTSMKGTQETCDKIFAYKTGSIDNFTTNQCYNSSIATENDYCKIKTCKLAENQNDGTCGSFLEGCVYNGNGGCVDPKANDTTCASYTGVLAFCESAIVGSNSSKYCFGTSTSATCTVRTCTDNKTATKDEECESFMSGCIAKSEGGCINRQSRSCSQQSGTVTSCPNFSGGLQVSSVWNKIACTKYDACQDRLCSDKTSPQSAQDCLDHKSTCRFFKAGSACINETACSSYNLPDTATNDQQKFDYCTSIKDNFGLLCGYTLGATKCSQRTCEQFLSTYTSLSCTTYLQKNISTSTDDTCKLAGTTCYLPNKKDCAYSYTSTDTTDTLKLAQCRRFVNDQGHQCSFKKGDTKCSLQDSCEKLIDQRDARTCNDQKHFRSLGLCQKTTDSNCLTLSLICSYYKFDSTLTTIQKQQFCWGLWIADDPTRIATPLAYYKKCVFKTGSSCEVLTDCSDIYSASSFLECSILLSGCLYFRGKCYASVSSGNCPSTFPPGITTDSQKSMYCRSIKEDSDYCRIKSDKSKCELATNADCASIQIPTSGSWDSFSDPTSNAFKSAYCLAQSYKNRSKLCKYTSGSNCSDATCADIPSPTSQGDCDNYIYGCMYFNNKCYASGSFSKDIDCADNNKVPMDSGLTQTQKLAYCQLYVSTSGTLYCTFDQFNPTSQTQCVIGSSCNAYTNLPNTNDDDRSTYCLNKINKYGRSCAFTEGDTTCRDFNCQDIKNPTNQIDCDQLSWGGYCAYSQGRCYKWTDDCKNILALSGNEQFYCESLLTTKQCTYISGTKCTDQTECHLYSVNENQASICAKLTNGTDKCTYISGNNCVILGACESYDGTDTAEKGPAEGSEEIECKAVKSVDGYPCIMDSIKKCRTQVCTDNDASATDCINNAPDCLYYSNKCIKKDSCAKYTPQGDNDTSKQIWCEGVQNDTGDFCAWDSANSKCRDRSCSDKAFYTDFDCQSYHKSCKTNGTICIAQSRACTSNNGSADFCNSLLDSTGKDRCRPIATATTTSSPCTIRTCYDNVTATTDSECDTYLSGCVTRGTGCIPNSEPCTSYRGTKQQCEQFKKYTGLDDKKNPTYEYCSGDAGNIVISKCKVRTCADNTTATSDTECATYMNGCITKGTGCLDATSSCTVFKGDQATCAKFLGNSGRDYCWNTSTALVTANCAKKKCSDVLGKNNKECTDGMPPFKITDDPFCVFDGTSCIDYGKNCNAFNGNEETCPTYLGRDGPCKATTVGTLKGSCAKRVCTEAPNSLTTDADCQKYHKDCYTTGYGCTSTKQCFNLTSQTACKLREECTWANQCTNAITSCSTQSNTNYSQCTNSKVNGKFCAWQESNSTCRAQVCEDQPATITSHAQCQNFADTCTTTGAGCITISTCTLYKTQSICIAASTTKDGVGRCGWDVTTNKCRNRICSDRNGITDEECNIFLSGCKTNGSGCVTGASCTEFNNKQFCIKSNSGPCLWVNGQCYDYDRCEDALKKSHSECQAFSPLCTTNGDTCIPITSCANTSLKASCVVGTDGSCGWLPTGKCQKFGLCTDAVAATNDECLSYGSICITDGTACLAKSTCGSYKTQTACNNNGTDGICFWNATTNTCKLKECGDEQKGTNDQCKLISVTGGSCTTDGTKCIPISTCSNYVEAGCFNGTDGECTFALPIGATTGNKICRLKQCEDISSGTSNANCTGVISRKSCVSNGINCISKSTCSTYKTITACNGGGLENNKSIVCAFTPNETDKVNGTCKTFSACTDASKDKLACTTNPSCKWTENSTGTSCVNHSCDTFATGTDCQPIPSFDGTSSTICVLQSGKCAATDPGTMTDSKICYTKSAYTYSWNAATNKCESCLSGSVNPNVSNNTIDNGVTTESAYILSAVSLGILGLMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VGU5</accession>
    <name>A0A8S1VGU5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0630140</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VGU5"/>
    <dbReference type="OrthoDB" id="6079689at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47176:SF1">
        <property type="entry name" value="OS04G0577500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47176">
        <property type="entry name" value="OSJNBA0020J04.13 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005902">
        <property type="entry name" value="DNase_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8175605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="254" mass="29766" checksum="3A9BA73C53F91737" modified="2022-10-12" version="1">MELQKYADAHSHIQDFENVDQIVKESVESGINHIICNSTTFDFLPPSLLNLYPFIIPCIGLHPWYINKEQNIEYEKMENILKLDERIQVGEIGLDFIKAKEKEDQKRQCEVFITQLQLAIKYNRSMSIHCVRASGEMLKIFKKQLKGQNYNASIIMHSYGCPKEITGSLDKLYPNFYYSLCLNMKMEQLNNIDKNKLLFETDAPYQYNPKIVESDDQKSHPKFLIKLIQDCATYLNEPLLPQIVYNNFRRALKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VHU4</accession>
    <name>A0A8S1VHU4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0650258</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000065">
        <property type="protein sequence ID" value="CAD8176784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VHU4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8176784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="65" mass="7719" checksum="0E72EA074BC6FF2A" modified="2022-10-12" version="1">MARIIYCLKKNKTITGSFIEKHILDGPSSTRIIVIYFQNNVSSNKENYYCNCQEQEMELIDWRIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VIP7</accession>
    <name>A0A8S1VIP7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0630162</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VIP7"/>
    <dbReference type="OrthoDB" id="291032at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8175649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="226" mass="27276" checksum="AA25A2E140EB9677" modified="2022-10-12" version="1">MKSHSQHENGTLQQLGRLRIPKVLFLPGEPMAKQRVDSLPSSPVKGIPNQADVQEKLKILQAEREARSKLQEQEMFEREKRSSFNRQAHIINKLHNVEQQLEKWKKTRHNIEFVNKTKMSQMIECQQKTGVEFHRSIQQRNKSYSDFEHRSAGIYLNHEIFQRRKEVERERNQLVNQTRYRNNVDQIDRNVRIMQKKYRTEKKCFALRKPFYNINDLKNYSHFLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VP02</accession>
    <name>A0A8S1VP02_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">THIF-type NAD/FAD binding fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0650111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000065">
        <property type="protein sequence ID" value="CAD8176496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VP02"/>
    <dbReference type="OrthoDB" id="10252231at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="1">
        <location>
            <begin position="2"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8176496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="177" mass="20252" checksum="06B00C65928A5866" modified="2022-10-12" version="1">MAPQLNIIAHFAVIKIPGYTMDFFLQFDAVIMELDNAETRSYVNKVCQALLIFIVDAGSMRFKEYANGYSEGTVSCTIRSQPSNCTHCVIWAIYLFSQLFSGKVGIFVVKGFDQNQPNSVFNKFFKGEEMINSIDIIQNNMNLLRSIILQREKNLLRNYKACGFMHIINSIIWIIVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VS29</accession>
    <name>A0A8S1VS29_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0740110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000074">
        <property type="protein sequence ID" value="CAD8180414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VS29"/>
    <dbReference type="OrthoDB" id="293765at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8180414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="420" mass="49446" checksum="68F9152B64B1D889" modified="2022-10-12" version="1">MIQQDPQDNLSLKILLQSSIKFKTETQSKFIQKLIPLNENQIFLYFEGSLYELYDIESQQIIGKGQIANDVKYFTQIYPQTLCFLDPEDMKVKLLDLKTHQINQSFVFAASNVVKQNSDKQLKDALSDKFRIMILDRECDNVRYLITQDLEKQYSNNNICHFIIRIIPQKYTHESEFNLNPVFEYNYPNQTGQTDLNNWQVDQETFAVYGQDFEVGLAEILLIKPHLMDKAFYIIHIKDDISFTLINVTNWIHPHLIALWQNNNDGNAKPTIFTIDIRSYYNGQNWLPEPDYNATKVRDDYVGDQPVTFIDIYQFQLLQTYVLQFRLIGNDPKISLINYANIDKPDLINELKIADFFNELPSNRSYLLLYNDVEVKNGIVEFNIVFPVDARHQLLQIMEKAKIMDKYGINNVEELFEFYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VU12</accession>
    <name>A0A8S1VU12_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0720131</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000072">
        <property type="protein sequence ID" value="CAD8179615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VU12"/>
    <dbReference type="OrthoDB" id="295152at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="47"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8179615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="250" mass="29020" checksum="078FF623D70EB38B" modified="2022-10-12" version="1">MNINNQLLKIVQDIQAVIKSEYEIKPNLDDILKILKQQVAILIELVQQQKGVEYDQLEKAVQKAEAEIRNHVRLEQQMKLYSDNLQEKIDQLEKEKLELLEKNKSLTAGQERKISSQNQTRESSPSLFKKSYILGNQVYNRQQGNQQEGCVQVAQNCDSQQTRERRSSGTQHSYMMMKNIREKVNMNASQEKVSDKSSTILKSQNSTSLLKMYNIQKLIIKNQQDKQLQQLKNKTINQILDDSQATKKKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VU80</accession>
    <name>A0A8S1VU80_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0720176</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000072">
        <property type="protein sequence ID" value="CAD8179705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VU80"/>
    <dbReference type="OrthoDB" id="286463at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="102"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8179705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="184" mass="21296" checksum="EC0BE381D5AFB0D9" modified="2022-10-12" version="1">MNQQNLDKDEIALLQKPWVKNHTTLTNDDILELYRLFNLYCNPRTRRIDLKDVMITAQHLGLVQKSPIVANTLQQVSDQHGDGGVDFEEFVRELTNKLGNLFDQKGREQLFTLVDIDGKGTLDKDDLRKISDELHLNFSQKDIDEIIHNVAGYDAVDITAEQFEKYLGKLTNRRKIETEVLRTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VVA2</accession>
    <name>A0A8S1VVA2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0720178</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000072">
        <property type="protein sequence ID" value="CAD8179709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VVA2"/>
    <dbReference type="OrthoDB" id="342730at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19756">
        <property type="entry name" value="Bbox2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047153">
        <property type="entry name" value="TRIM45/56/19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462">
        <property type="entry name" value="BONUS, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462:SF296">
        <property type="entry name" value="MEIOTIC P26, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="176"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8179709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="466" mass="54417" checksum="2AEC5E7712422B30" modified="2022-10-12" version="1">MSEEEIVACDQCKQVPDNYLSLNCNHCFCLICMAQNFLKGGRIFKLEGTDNIHYICTVCEKDTPLDDGSVSVVESICHQLIQTSLEKVREDQNQIKEDKPLQQSQTIIQPLPQPKCDIHTKEDATLFCFTCENKCFCIKCLLRHDNKTHEIQNVNSCLKRLKSKMNDATHTIGASLELMQLQLKRVQDQDSTLQEEMDYLIQRVQEQFQSLYQVIQNKEKEILTQLDNLKEIQEQQTEKMLNDIQMKINSLIQLRSDFENIGLSESLQPSVSILNYYSGCKQIMQKVIGQMSFSDIFSTVKYFPDNEQFFRYDEFELKMQNLKAQIDRFFNQQRSVSQHEKQSRITQKSAIIDQLYLSQRCHTQMDESTDSFKSHQVSTYSKINTPINNQRNLQAKVDEIKQLYSDRVKTQQKLQKQQENSLNNIQRSFSELASFKKQTALSALRLQQGLFDSRQQQIQESQQVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W1U8</accession>
    <name>A0A8S1W1U8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">B9 domain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0800090</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W1U8"/>
    <dbReference type="OrthoDB" id="184109at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036038">
        <property type="term" value="C:MKS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010796">
        <property type="entry name" value="C2_B9-type_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12968">
        <property type="entry name" value="B9 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12968:SF2">
        <property type="entry name" value="B9 DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07162">
        <property type="entry name" value="B9-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51381">
        <property type="entry name" value="C2_B9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8183121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="235" mass="26558" checksum="57F749FFE64C15B5" modified="2022-10-12" version="1">MSMPYQQTGNQLDYQEVKQTKADLKAEVHLIGQIVGGLDFQTDDGLFCELAIDCGDGWDLLQPGNNKGIQTQTSYANPGQLFSWGHPFDLHFSVSNIIGWPKALLKVWRLDSSNKIDACSYGTAIFPRSAGYHQITCETWTPTGENLDLEIRKPNEDLATNTDQFSKFDRSLDWRFQALSFYMENPPRLTTLAPLTTDKGDFARRKILNSISNGRIIIEVEVILKNFKRLSFSGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W1X8</accession>
    <name>A0A8S1W1X8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Catalase core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0780044</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2 H2O2 = O2 + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:20309"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="EC" id="1.11.1.6"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the catalase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W1X8"/>
    <dbReference type="OrthoDB" id="6880011at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004096">
        <property type="term" value="F:catalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08156">
        <property type="entry name" value="catalase_clade_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.180.10:FF:000001">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018028">
        <property type="entry name" value="Catalase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040333">
        <property type="entry name" value="Catalase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024708">
        <property type="entry name" value="Catalase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024711">
        <property type="entry name" value="Catalase_clade1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011614">
        <property type="entry name" value="Catalase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002226">
        <property type="entry name" value="Catalase_haem_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010582">
        <property type="entry name" value="Catalase_immune_responsive"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11465">
        <property type="entry name" value="CATALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11465:SF9">
        <property type="entry name" value="CATALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00199">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06628">
        <property type="entry name" value="Catalase-rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038928">
        <property type="entry name" value="Catalase_clade1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01060">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00437">
        <property type="entry name" value="CATALASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00438">
        <property type="entry name" value="CATALASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51402">
        <property type="entry name" value="CATALASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="7" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Catalase core" evidence="10">
        <location>
            <begin position="10"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8182139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="488" mass="55797" checksum="70A61C13BD5FC9C4" modified="2022-10-12" version="1">MSDNSDNVLTQSTGCPVDDNQNSLTAGEYGPILLQDTHLIDKLAHFDRERIPERVVHAKGAGAYGYFEVTGDVTKYTKAKFLDTVGKRTPIFTRFSTVGGEKGSADSERDPRGFAVKFYTEEGNYDMTGNNTPVFFIRDPSKFPDFIHTQKRNPQTNLKDANMFWDFLSLVPESAHQVTILFSNRGTPDGYRHMNGYTSHTFRWVNKEGEAFWIKLHFKTDAGNKSLTGAEADELKRTNPDNATQDLFEHLAAGKTATWTVQVQVMPEQDGFKYKWNIFDVTKVWPHSDYPCLNFGKMVLNRNPENYFQEVEQSAFSPGHLVPGIEPSLDKMLQGRLFSYPDTHRHRLGTNYTQLPVNCPYRARVINQQRDGPQSFNNQGNRPNYEPNSFQPLKMIPETKTSQYRVTGLIGRYKPSHPNDDFAQPGVLYRKVMNDFERQWLITNIVGHLKNANRVIQERQVKVFTKCDPEYGERIAQGLGFPNNKARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W2J4</accession>
    <name>A0A8S1W2J4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase M14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0780140</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the peptidase M14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W2J4"/>
    <dbReference type="OrthoDB" id="10253041at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050821">
        <property type="entry name" value="Cytosolic_carboxypeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040626">
        <property type="entry name" value="Pepdidase_M14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756:SF45">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE NNA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18027">
        <property type="entry name" value="Pepdidase_M14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M14" evidence="4">
        <location>
            <begin position="208"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8182329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="675" mass="79046" checksum="8D8280016FCF1ACC" modified="2022-10-12" version="1">MDDEGFESAFIETLITQQDKQPTFIFTSLPTPNPIVYQSDYRGVTNDIKDIEKTYKCVYKKQYGSKVSNQLFKQLNLNDLQTPTITQTQQLPQFDSNFTCGNLAAVHQVDTFEYDLLLQEDINTTSYSQWFYFKVTNPNEYKYRINIINLSKNYLQYQNGFGVYIKFDTNWEILKSQVLIKQSQYVKSNFCKTTSTKLYTLSFWINRSSKTFEIALGIPYTHLDLDRYLLKFQSEILTNTITGLPVRMVQFGSKRSDVILVIARQHPGETVSSHVCQGFLDALSNDKTLQLFYNIIVIPMVNPDGVKWGNFRCDLSGKDLNRVWQNPRSKYHQQIIQIKNLIQKILDEGRDIKTFIDLHGHSRKLGTFMYACRNTDDPIQCRVLPMIMAENSKYFDFNSCTFNLKSYKLKTARAFVYNQMQKHNPNSFHNIVTLETSFFGYKLENRFIQYKQNDLIQIGKDLHQSLKIYYMQLDQKAKILKDLESNKQYYQQIDENQLSDDSDSDAEIGIIPDEDNPSQTIFTHKSVKLFHDTPKTSSLKKRNLKKAINPFLVGQCEKKYNFHKLTRGESKSQKTINQTLSIQQNQSPSIKNLNKINRNLSSHNKDHIHMHSSNVDPYESVQSCRIQRQNTTRTPKEFKLKSEQRLQYHSPNDKLIVFEQNFKIKRKNTQTIKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WFH4</accession>
    <name>A0A8S1WFH4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0890070</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000089">
        <property type="protein sequence ID" value="CAD8187425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WFH4"/>
    <dbReference type="OrthoDB" id="300277at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8187425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="348" mass="40931" checksum="B5287898462CF169" modified="2022-10-12" version="1">MSNTKLTQLILLRKHSEADTYYYTKSLNDFIDNARSTQVINFHYILNLNDEAEYLRRTYTINEIVNKITSLVEYYKFHNDIPRCFIPKISDIMSNYYDKRRKIEYLRIKRMIEYQNKIHPDQPQKKIIGEISEMSITQKSGKKYSNILNDIIETSNTIDKIKFELNSIKLNVEELQIIQSNREQQQLDSFLKHIAKKKQKQSQVPNSLTLKFPIGLSSRTIKQVISRKMITQQQTQITNLNSAKTSKQLSQYQQSSQIQPSQKINNKQLPTQKDFNHFGSFTQRIVKDNPLSKLLSPLHIQLVNVNNPSNKCKTTVSISKQFKITDSLKLSTKFHQHTKSDFQFFRKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WFW2</accession>
    <name>A0A8S1WFW2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0900106</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8187911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WFW2"/>
    <dbReference type="OrthoDB" id="302357at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="914"/>
            <end position="957"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8187911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1084" mass="131963" checksum="002E1B13B810212E" modified="2022-10-12" version="1">MNICLLSLSNQFKDFIYLIKKILKHHDGITSVSQRLYKPPPTKSDYQKKQLKRPSHVFHNQQRPPKQQIQQQIQTPEQLLNSIVTIISDDLDPPQQQNQDHHTTFQQIPQTFLQTMTYNLKEQGLINKLKNTLKDQISFLQDSLNFYNNINQKYEIIQTSKINLEANDKTQRLKAWSYYKANQQIKLINEKIDLFSIPIGFKIQSDKQYNTTKEQMKNIIHQHIQQLQISQDALNSYKFDNFFDNQNVDKIFNFLIQFQEAYEKCENTKQLLIIDNSLCDALHTLRIQSSILYEQIKVQYEDWYNKFQEKDGQYQLFLKMLQIRIHHMPFYYLTQTPIYESKHHQDVLKYMDEMLIIQQSLNHYQDQIPFIFKFFKLRNKQLKILEIIINYVNSNYPYNKSLIIDVFYLKEVKFLDEDLTTFDYLLYLSTQIKELQDKLIQKQKFVSSITLQILDLSKLNTEEKVIEVKEIKKAQKKEPKQTLLDKQKQYIKNISDKNKSEEQFIQSRLNKQDEKLKIEKENKIERLKAKLLRQTIAQKQEDKNYEFLVKKYRYQENQSNIIIEKEERKRRNFDILGQMLYLILDSRFKLYKIQQKETYDQIINFYSQHMYQKGIPKKFKLSTIQQLYESIYELSKDFRDHFNQYFLDKQVKQYLNLVNQQQNQQVNQQSQLFQEAKQIDFINFSLTISLKLLNIISYHKSVYTINRFIDFDILQVPDLDFYSLKHYLQWIQKNAENTFDYLQKNSDNLLRFEWLPFLNGQVKCSERQQEKLFELIKKINHCYILEFEVIRFIINTLSRELFHENTFKFDLRELTKNYIEKSIKQQLQDKIKYFPKLNQELLMQIEPFLQYLEQKVLCNLEAFYIISIIQKETVQNNEFEQIIKRNEYFHDVNDVWKFKYQGMRYILEKNEFKNNNLNEMINDQIRRIQNVKINNGRSSLQEKYQELIQQNNQKNERISVKKPLNMILEVDYKENQKFVVRLVNESNQKVKMEIYEEMSSDVVELKWDKDKNKDKWVIKINNEKINFNQIDVWTYDIPKWFNLIINGEKRNIRIDQNILQLDRYLMVRYNNESEKELFNIYQYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WHD8</accession>
    <name>A0A8S1WHD8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Geranylgeranyl transferase type-2 subunit beta</fullName>
            <ecNumber evidence="9">2.5.1.60</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0900169</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the transfer of a geranylgeranyl moiety from geranylgeranyl diphosphate to both cysteines of proteins with the C-terminal sequence -XXCC, -XCXC and -CCXX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>geranylgeranyl diphosphate + L-cysteinyl-[protein] = S-geranylgeranyl-L-cysteinyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21240"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11537"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:86021"/>
            <dbReference type="EC" id="2.5.1.60"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the protein prenyltransferase subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.60" evidence="9"/>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8188037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WHD8"/>
    <dbReference type="OrthoDB" id="5428259at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005968">
        <property type="term" value="C:Rab-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004663">
        <property type="term" value="F:Rab geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02894">
        <property type="entry name" value="GGTase-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000012">
        <property type="entry name" value="Geranylgeranyl transferase type-2 subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045089">
        <property type="entry name" value="PGGT1B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="Prenyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026873">
        <property type="entry name" value="Ptb1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774:SF11">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE-2 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 9" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Prenyltransferase alpha-alpha toroid" evidence="10">
        <location>
            <begin position="24"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8188037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="321" mass="36156" checksum="C4DB174A6613B6B2" modified="2022-10-12" version="1">MFQQQLVNHLPYLMALDKSKDLESIGYYLSEHLKVAGGYWTINAIACLNKLDDISQEKKQQLSKWLKECQNQDGGFGGNTNHDSHITNTHYAILLSFLLGCELDYEAAAKYVAARQRQDGSFEGDQWGEVDARFSYCGLSSLTLLNKRDLIDVKKAASYIKKCRNFDGSFGGIPDAESHGAYVFCCVGTLYLCEDLSFNIDELSMWIHERQTSKGGLNGRPEKLADVCYSWWMYSALCLLKREQWINQQALENYILECQDNVGGIADRPNNQPDVFHTFFGLAALSLLNGDKYQLNPIDPAFALPKSILINIPGASRFIQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WKH1</accession>
    <name>A0A8S1WKH1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0920025</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000092">
        <property type="protein sequence ID" value="CAD8188659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WKH1"/>
    <dbReference type="OrthoDB" id="71437at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="331"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="375"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="175"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8188659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="553" mass="65454" checksum="970419EBC126FE98" modified="2022-10-12" version="1">MKSIMGRCRMIEDLDKITCKGQHNDKISLLVMLENDLQNEQRLYCEKCLQTKKGVLNAIPFDEAKSRIEKQQNRHFSELNYSILQRIKFIDEFSENIIRVLQTIEQFIKDCEQRIKLWQDQLIKFRDDNYTYSLIQELSNLKKEENQWVQEKYLDSQNQINLIDQDHSLHLKFTVDEIQQQVQNIVKAHELLQDNIQIHFKNLINQSQKEQEQEQLQKENIQLSQRFFNKLFNKLVQEIRQNEICYAIGFNQNDSIMAAGFNECIKIWKIENGQFIDSQIILKGKSEVDFAICITFSKRKNLFVYGSYDHSIGIWNEIENGQWNPSDSSNEMSHSGMVTCILFNQCEDQVISGSQDKSIIIWKFNGTSIKFDQQLIKHKNTIFNICMNQQETEIASMSKDQQIIIWVKISNKWKIKQIIQQSNINFGDIVTFISNDVLVLYQNFQKFTYARSTIDTHFTQISEQSLDLTDIVKNDEQYLFPTFHFIHKNLIVQKKDGNVYFVVVDEDFQPLSLSKLYINQKNTFYGNITNNGRYLVIWSFGDLEFKIYKLSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WN44</accession>
    <name>A0A8S1WN44_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0970028</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000097">
        <property type="protein sequence ID" value="CAD8190612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WN44"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF32">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="13"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="290"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8190612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="446" mass="51561" checksum="50E9CED07CF7CBED" modified="2022-10-12" version="1">MEIEKSFAKYAHYTVKQTLGKGAQGKVKLGLDGDKEVALKFVQFSKEIEKEMQIHRGLNHKNLIKLLNFHVNEEYTKRSGKKEIKSCLVYDYMKDGELYEYLSQTGPFKEETARTFFHQLIDVLSYLHGQGLAHRDLKPENILFKDGQLKVSDLGFATFVGGNQGDGVLASFKGTISYMAPEILVRQKYVGQSVDIFAAGIILYLMMTAQLPFKQANAKDQLYNLICTNRFDQFWAYKEKSGIPRFSNELKTLINSMLAFDPLQRPTLSEIISTSWYQGATISNEIVVKLLNARKLKIQAQQDKENLQKLLDKQKRQAANQNQQLTVGIGNFKRGEEDITIDLTIKRELKRFKQTDVILPFEPNQIFVKLIENMDKFANKINYVDDKKYRLHYWFLQNNEEFEMAIQLVHGPVEFTVGLDFELIKGNGLEFYQKKQQVEDMLLQIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WTW4</accession>
    <name>A0A8S1WTW4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1040153</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WTW4"/>
    <dbReference type="OrthoDB" id="10411012at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8193564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="132" mass="15758" checksum="253E678D878CCE23" modified="2022-10-12" version="1">MKRFLQLPQCTDDCFLQLIQGSCLDMAIEKQWETSIKCQFRYGEEEQIMDIKKKNKETYGFMNLPNNLKYLFLLNLSKCKIHINRRCGLLHLSEYHGINFYDILEKAIKTKANTVMKRSLEQQIQKILKYVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WU02</accession>
    <name>A0A8S1WU02_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0930096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000093">
        <property type="protein sequence ID" value="CAD8189226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WU02"/>
    <dbReference type="OrthoDB" id="1923159at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12438">
        <property type="entry name" value="RRM_CNOT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034261">
        <property type="entry name" value="CNOT4_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039780">
        <property type="entry name" value="Mot2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12603">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12603:SF0">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RRM" evidence="1">
        <location>
            <begin position="47"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8189226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="278" mass="32486" checksum="2CD67C3FB08FC796" modified="2022-10-12" version="1">MQNSDNSDEYYSDDQQESPRFLYSFKEISLQNKTLLAEIRIICKNLIYVIGLAPTIAKEELLKKPEYFGQYGQIQKLIVIQSNTFNPPSHAAYITYRNEQEASMAILASFGTTKYCTNFLKGQQCKIKDCVYLHQHPKDKESTQVIKKEEMNNSKWLFSYSQKIAQENFKKFYTKINYKNALQKSIFLNTQNILDRMIEDNIVDPIPEQPQPPQQQQIIEETQSIEIPIQQKPIDIEKRLEVIIQKMDGDSNSRFKFTNGNCPQDYEAIQQLKQSLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WVZ3</accession>
    <name>A0A8S1WVZ3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1040150</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WVZ3"/>
    <dbReference type="OrthoDB" id="304987at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8193562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="233" mass="27380" checksum="5CC9C50867823BFB" modified="2022-10-12" version="1">MVEYPKISEILADTGSIISWILSISFIVSKYNENLSLEKTQREIITMYYNDFNDFQIQKNCLGKVQRVNYKGKEYDLQKSEEILNRLDRIAIEKMNYLNLQNEVAKLQLILQEHLGLQQIKKYLESKFTLESLFDKLGIPEKISQQEINQIHAQQELQQEVVQQLNIEGEIDSKFNKDIVEQEVELRMGLLDMKTSENLQFREQNSNLQIQESLQQINNYLTVINVDKTDGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WZC2</accession>
    <name>A0A8S1WZC2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Aminomethyltransferase</fullName>
            <ecNumber evidence="2 7">2.1.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5 7">Glycine cleavage system T protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T1040131</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="7">The glycine cleavage system catalyzes the degradation of glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[protein] + (6S)-5,6,7,8-tetrahydrofolate = N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16945"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:10492"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="2.1.2.10"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the GcvT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.10" evidence="2 7"/>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WZC2"/>
    <dbReference type="OrthoDB" id="10263536at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004047">
        <property type="term" value="F:aminomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006546">
        <property type="term" value="P:glycine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006223">
        <property type="entry name" value="GcvT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028896">
        <property type="entry name" value="GcvT/YgfZ/DmdA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013977">
        <property type="entry name" value="GcvT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006222">
        <property type="entry name" value="GCVT_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00528">
        <property type="entry name" value="gcvT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001567">
        <property type="entry name" value="PRK00389.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43757">
        <property type="entry name" value="AMINOMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43757:SF2">
        <property type="entry name" value="AMINOMETHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01571">
        <property type="entry name" value="GCV_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08669">
        <property type="entry name" value="GCV_T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006487">
        <property type="entry name" value="GcvT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="GCVT N-terminal" evidence="8">
        <location>
            <begin position="16"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Aminomethyltransferase C-terminal" evidence="9">
        <location>
            <begin position="301"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8193529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="385" mass="43768" checksum="EAE7395530277A3F" modified="2022-10-12" version="1">MRKISYSFTQLMKLHLHDYHVNLKAKMVPFAGYEMPVQYPQGVLKEHLYCRESCGLFDVSHMGQVKVFGEDRMKFVETLTTGEFQTKKSGQSVLCLILNEKAGIIDDTIVAKRDDHIHIVVNAGNKFIDMKQMDKIIKDYNYKVQYEYLQDKPLIAVQGPNAHKVLNEIFGTEYNLDKIPFMFMVNIQKNGIDYQINRCGYTGEDGYEISVENSKAQELCDKLLATKMAQFCGLGARDSLRLEAGLCLHGHEMDDTISPYEAKLMWTVRKPNKETGKYNESAAFIGRDALPQRQKDAKFKRMGFITQSGIARPPCDIEFQGQKIGSVTSGTYSPNLKKGLGFAFINNEYAKDGTQLQADIRGSKVNITLSPTPFVPQRYYKPEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X129</accession>
    <name>A0A8S1X129_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1100167</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000110">
        <property type="protein sequence ID" value="CAD8195834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X129"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8195834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="84" mass="9754" checksum="63E131A793F0CFB5" modified="2022-10-12" version="1">MKSITTQVNFQFSSSNDFQKLGSLHGFWQNRIEIHSKIDACNNKGFIFVLIQIKTIPGLNSCIRHFSYFIIYLLYEGPILILNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X3Y8</accession>
    <name>A0A8S1X3Y8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Proline dehydrogenase</fullName>
            <ecNumber evidence="2 4">1.5.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1130106</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Converts proline to delta-1-pyrroline-5-carboxylate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a quinol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23784"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.5.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the proline oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.5.2" evidence="2 4"/>
    <dbReference type="EMBL" id="CAJJDO010000113">
        <property type="protein sequence ID" value="CAD8196713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X3Y8"/>
    <dbReference type="OrthoDB" id="5464at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004657">
        <property type="term" value="F:proline dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010133">
        <property type="term" value="P:L-proline catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002872">
        <property type="entry name" value="Proline_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015659">
        <property type="entry name" value="Proline_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13914:SF0">
        <property type="entry name" value="PROLINE DEHYDROGENASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13914">
        <property type="entry name" value="PROLINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01619">
        <property type="entry name" value="Pro_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 4" id="KW-0642">Proline metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proline dehydrogenase" evidence="5">
        <location>
            <begin position="110"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8196713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="514" mass="60598" checksum="FA61299EC86908DF" modified="2022-10-12" version="1">MISSCQRFLRSSNVIYNFAVANQRRYSSSQLQYYQSLRIHKDKSIPSILNNLFINKMLQYDWIVDQGPKLIDFLYTICGTKLTNFLINHTIGKVFTAGENLESVERHLSSSNSKISYIMDYCSEALEGIKDYEKFYDENSLIFQQTILECAKKPEKKNMIAIKVSSLIDLNLLKQINKARLNIFDMFYKISQGEQTITIQQVFSYLKEQGISLNEDEQKQFIKGVLKFNQNDIKIDQILIDEITWKYRVQPIFMFDLDLNNNPVIKYFNNLNQKDIYLFEQFIERIKYFMDPALINQVCVMVDAEQTYIQLAIDSFSEQMEAYYNQNYTIVFNTFQNYLKQTKQRTDYEIEKAEKFKLNIGIKMVRGAYMVEESKLAKQQNKENPINNGYDTTTSMIERNLEILIQNIHKSPTKVFVASHNEQTIDYVKEIMNRYSIPNQGDVLFAQLYGLSDHVTYQLASEGYKIYKYVPFGKTEIMIPYLMRRAQETKKVLQSSSLQTLLLIDELKYRLYFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X4B6</accession>
    <name>A0A8S1X4B6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1090089</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000109">
        <property type="protein sequence ID" value="CAD8195379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X4B6"/>
    <dbReference type="OrthoDB" id="310171at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8195379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="313" mass="36513" checksum="6BCDD90C9B2F02AA" modified="2022-10-12" version="1">MSKFQDDMGPSQNQIRQRKALENLINQQNKSKSKQTQRTHILVQQLKPQNCMEKLNISQLKSQKMEMDRKIIKNSLLQDIGNIELEIQKMTVEYKTPVNKTQIIDSNNGLFNQKVIAYQLNQTDVSKSDHKISLIHRHQTSNDNTTKLTKTNLISEASYLKLAESMRNYSNQKKSVEVKIIDKPKTKPYNILSTKQQSDINIQKKQNCDVQNSIKTQIPQDNRLSQKINSERNKTTQIKSILKQTSFHTQDINCIQHNLISNVQIKLDNEHNQIYNKFNDDNKLDKALKIYNTLKQLVHKDSQSQTQVREQKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X689</accession>
    <name>A0A8S1X689_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1060037</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X689"/>
    <dbReference type="OrthoDB" id="306165at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8194126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="99" mass="11589" checksum="F49BA810A25F6A97" modified="2022-10-12" version="1">MNDTNDVQILTDQINDREKLIIHYLNEDLKLEQLTDPQKKQINFEVSKKKTIFSQKTLDSDSEISEFNSSSTIKSTQLKMNKSPLLIRRLSTIMLEQIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X7D4</accession>
    <name>A0A8S1X7D4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1070121</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000107">
        <property type="protein sequence ID" value="CAD8194706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X7D4"/>
    <dbReference type="OrthoDB" id="6344460at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053233">
        <property type="entry name" value="ABRA-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21715">
        <property type="entry name" value="RH04127P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21715:SF0">
        <property type="entry name" value="RH04127P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="2">
        <location>
            <begin position="50"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="276"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="498"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8194706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="754" mass="90491" checksum="2E3306BCEF490434" modified="2022-10-12" version="1">MRAEFQILEEEIDEDYEPSREEILEYAECLGMSPRSDTKYFHIAKEGLKTPLPPPWRPAQLRNGDIVYYNPVTKVLQQEHPLDEYFKRKYIEEKKKDVQNAQPSKTSKKLKGLCEQLREPTTQETTQKTEKKLSQAADISHNNLSDVPLLNESVQLQEQFELIDNQFKFELTQYEKEQESFVMLYKINRAKQFEQDKSEISDSSFEDFNINVPEKALQIRQKFLNQKEELKIKIQEEIDQKLIIEQSKIQEQSTSKLLDYRAAKQIKNEQRIQQLQSEWESKLQNVKQRIKSSQKELEDKKLQQQSMVSQQQQNLQTKWLKIQGQYDKQIQKEYQQILFNMDEILKQKKNDIVLQLKQFKLQKSQELHNLYKENSEFNIKKRLREIYREKLELAKVSNLHDMENDLKQEEISQYEQMTENIINYKNMLRTNFVKLKQQQIDNERVYLNNYYKEQLQAFKVENFKCTELQIQKLELSQIVMANQSFSQLDNSQLKVQINPDIRIQKKELELQIQEYRTKIENLKAKFFNIDQSKRDEEFLIKVLSQNDKIDFLQQSLQEQDNILNELTKKYVSQPVMTNKGIQQTPKLQEQDQWVNNVEQEIQWLVLQKERLKYDKKKLQKSYEKLSLLASTNDQVETYNKCVNVVRNNQQILKNCEYIITQLQFNCQQYKQTKLIQYLKENDKLQEELQTQKSLIGNISQIQLDTSFPTYKQVSQSFHQSDFDQSYLKQQTNNQKQLLRDVRDSLNKSMLSRMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XCR7</accession>
    <name>A0A8S1XCR7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1190077</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000119">
        <property type="protein sequence ID" value="CAD8198592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XCR7"/>
    <dbReference type="OrthoDB" id="297641at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAS" evidence="5">
        <location>
            <begin position="111"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="3">
        <location>
            <begin position="322"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="564"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8198592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="692" mass="80636" checksum="A06638D20E035A74" modified="2022-10-12" version="1">MTRVKLSSFTQTLIQEEFKFKQTQFEQDQLLSSESKRDFIKYDLNESIILKAFNGEIETPQQEHKYIGVNKDQMDQRIIVKKHPNNLPTTLLSDSNYNLEPLKLERNTSTKYSEIVSIMNNLPFGILFVDSSLKVLDFNQKVTQLLGLTNPCDMIAFLDQAIQSGDFCEFRSSKKVRKPQKSPSKMTFIKQPSISPQKIFLDEHISDVFSQYNNNASQFKGESDANIGKNLKSIFQNFKKIHQSQLTSLSRDNFQYIIRMDNMDKSGGKIKYKSLKLKIFQLDGFVQWESVVYLFFLENITKREEYKLLNHKYKFQQALLNSLCHELRTPINGTMSQLYALKDQLSQGLIESHLDPAIVSAKRLQFQLNDILDYAQIQCSSLIMNKSCFKLQEVYQQLYELFNFECIQKNIKLIFDNVSHISIYTDKERLIRIFINLLDNSVKFTNRGGTIKLITNTTPLYYKLLVEDDGQGISEEVIQKIEQQAELLFQDSLQYDSNKLGLGLRISQQLAKYLYKDQFFEIDSIYEKYTKVSFRVSNQIQHYSNEFEIDKHKFPQISDCDCSKILIVDDINCNHFALQVLLKKFKLKIDSAYNGNSAIDLVKERLQQQCCQTYRLIFMDIEMPEKNGFQASKEITQLLKEQNLNESCIITMYSAYSGDEDVLIASQCGMKERISKPTDIQKLEYLVNKYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XDD4</accession>
    <name>A0A8S1XDD4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1200080</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000120">
        <property type="protein sequence ID" value="CAD8198939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XDD4"/>
    <dbReference type="OrthoDB" id="415460at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="276"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="314"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="407"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="456"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="487"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="7">
        <location>
            <begin position="593"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="998"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1012"/>
            <end position="1033"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8198939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1177" mass="138051" checksum="14B1B9A354B0082B" modified="2022-10-12" version="1">MKKKESPFFGLDNPVYSQNISSPRFEELLPIYSNDNAIEQPIEEALKDKRNIFERNNFGAMKDGHKEESSEDDLYLSSVSDNRYDQESARSRTNSLIRQQRSLSIQKNQQINQNSLKKANTGLIIDSTFNLQVQGVKSSSKIDKYQSFVQQFELEEDKIRKNKELIQQKSNQTNELSSKRNSGINKGKIGLNGIWAVKGFIIIRLVSRFIQQLKTKTETIKFRLLTRKIFSVIGDFLILKYNFQKRATRLLHYLENCQDFLSRNIIVIRPDSKFKIIWDILLLLFIVMNIFYIPINISFNISTQGVFEYLFDLLPSWIFVAEIIINFNTAYYDKGLMHEDRKSIVKHYLKDNFFWDLIVVIPFLMSNMNIPFVRYTLLFRLTRLAPLMESIEEMLNLEENIQIVVDLLKLIFFLVLTGHFCGCAWHFVALTEHESFGITDNWLTHYDRQAFDYHWFDRYIISLYWSVITTVTVGYGDIVPVTTFERIFVIIVTLLLCGVFGYCISNIGNIFKQMSDKKTTYKFKLRQIHQHIRKRGLNLNLSLKVKKYFEYFFQLEQEEDSHAEIFISQLTKHLREEVLTDLYCNTLKKSRFLRENFKELTINNLCQFVKEKKVLPEEVLYSRFEQPTKVWFVLSGALEFVADHKNENDYYEATETFLKKVSSGAVLGEREFITQQPYEYKVRALKFTQMAYIDYEDFINVISENDSEYEIFCMKRDRLLFNPQYKGSGNVCEICEWTHNFIQCPFVFLQPNINKISSSFTAVRLNNRIKFPYRHTMKSRTRESLNDVQEGALKIIVGNLTEQEYTDDYLVSLGFQLTQNLEIDSQSGDEVSKNLNNSQSPTTSEMQKAFQKKNAKTMAGGVTMDLKDVKESHDLKKSIINFQRIQFGKEKQKALQLHKKEQQEKGIEVLEQSQILVLPNTITNNKFYQKQLQNTPRRSSNFEELGSLKLSQKLGNVTNPLDQDQNKCFMKQLSKNSEASEMQESLIRKQIENQERRVQIGGEQRRKKRKTTIQLGQKAQRRSYQNQQTNLSPGLQQQLQKQQLVTTLDQKLKRQSIGESKKQMQTNQITSVGQIEFAGELVENTQGDSLQQKILDIVHSNENFQVDCCKPTIMYFPEFNIEAILKKIDYYYLYVKGVEKKMYKRSKSNRNLFERIKPSKNATIKSFLNNSRSQQDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XJD0</accession>
    <name>A0A8S1XJD0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1270040</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000127">
        <property type="protein sequence ID" value="CAD8201253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XJD0"/>
    <dbReference type="OrthoDB" id="10249433at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052920">
        <property type="entry name" value="DNA-binding_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43358">
        <property type="entry name" value="ALPHA/BETA-HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43358:SF4">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="2">
        <location>
            <begin position="69"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="318"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8201253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="337" mass="39168" checksum="B8B768586AFB8245" modified="2022-10-12" version="1">MKQPSYRCTEKLWKPIVRPPRHNYRLKDMGNEIFMVSDTVTKRFDFEIQNSRRLILQCSLFEPIRMQDKPHPCMIYLHGNSSSRVEALTIIEYLIPQNISVCGIDLSGSGQSQGEYISLGYYESRDVIDLYEYLRDKKPFITQIGLWGRSMGSVTAILAANLNNNFKVLVCDSPFSNLTHLCQELASNNYNIPNCCFNCFWCIIKAKIRREAKFNIDDLNISQLIQTLPTDVSIVFLSAKQDQLIVEKHPKILMEKFRGNKLLKQFEGTHNSKRPLNVMKETVQFVIMQFDKYSWNQNQGLRDNNKIFNNQKDQIPIPDVDAPLLQPEKPYISKSER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XMR9</accession>
    <name>A0A8S1XMR9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1300170</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000130">
        <property type="protein sequence ID" value="CAD8202321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XMR9"/>
    <dbReference type="OrthoDB" id="302718at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="387"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8202321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="514" mass="60944" checksum="21BFEBE940C72076" modified="2022-10-12" version="1">MIRFGKSSQQTTKNPKKPDNLEILTLLDNCTLIFKDDRIALRNMVKDGNQDVISILNNYKKITNHKEMSTELRKYLRKKGEKENEKAPQCQSPKSNNNQKLFQQQPQQFPNSQKKSCPDLWKSADTSSPKIINLAKTKEITNDVQLSEIILTDRNQNETKDLEKIQYYSTNNQQYIQQLQPTFDKSERNSNNIQQDSEILYSQNQQFLEKDIDINEKNDYIESESSNESILKQMTEPPLQLIQDQTIKNDIKPTQLFSSQNSSYLQINIAQSYLQPALTQQPDLQQNFKQSVEQQNKERIQVGDPHQSYSFFNYQDCQILAQTQYKHLFTNWPTLLHKLDKPEYIPNYKSVLYIDTTPEYLFTLLFPNSAYIRINYFQFSNQLKQTSLQINIDELFKVLDKDGDDWINYRETGSILESICLQQEQPRNFIRELFESLKQPQSSSNAITLIDLQQLERIYQFQFARQLMQEIDIYQNGNLTYWDLFMHRDIYLMQKLCELVIQYQDQYNNQEQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XU42</accession>
    <name>A0A8S1XU42_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1340102</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000134">
        <property type="protein sequence ID" value="CAD8203644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XU42"/>
    <dbReference type="OrthoDB" id="331171at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF13">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="1"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="282"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="533"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="629"/>
            <end position="680"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8203644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="831" mass="100225" checksum="808A202651D831F5" modified="2022-10-12" version="1">MKKVELAINKLQYQPINDENHEDIQIDGKYYENIRQFDQQGCQIINFIGYSSDNLMQEIIIQTYQELKNDHLKVLEQIRKYQNQKEPKCQHIIYIIGLQDKIIISQLHNEPKTCVVIEKGQYNLLNLFDQIKSIHNIQKLKLFEQMVQGVKELQDLGYFHRYLQPNNFVFFENQNKEYKIKLIDFGLVINNNQTNKTSQNDNYNYMAPEVVKCDSKFDKTADIWSLGIILYKILTKEDLIQAKNRQEQKNQLCKLTQEWIDEYIIKTKDLKLFEQKLIKSMLQIKNTERIRLENLLKEINLQLIEQQKLDSQQKKEKKQQQVQGQDYVQDIKTQQLNQLTEQINTQSVFEHQKQINNQQEISTSINQIYEKEINIINDHLGKVQNKLNYLKQLLNQKIIENYQHIQENYDQIQKFLQDFFKSYNELYQQMQNSMKDTKPKIDQNQSQSKSQYQNLQYINELTSFYNGDNQTGMQLLQEKQIIEEQIIKKIQIDGISEQERKFQQLKNPLQNCFIQFQKAQQYFKLACEDIGCYSQMIDKRKNMEQQLQEKIKTLEEYHKIYLKNMELFQKEFDQFGKKGISQQQYEDIVRKQQQICEQKGTELCQVLNIVKKKQNELIFMPLTNPINIYEELKRQEDNFTNKLNQINQIKFDQLINPSSFNRLKQQILELTSKIDESIKNIPQNNQIQELNNIYKQYKEQYNILFGLVNYIKYYHLKRYDERIKKGQVNQQVVLNQELEVQDIFQRYLNFQFHDGNKILIETLIQDQEQINKQMNQKEDFLKSVNLNTFTQQMQDEQIINEIFNIKKYYKIIKFAQMHILYIIMKLNQHLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XUH4</accession>
    <name>A0A8S1XUH4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1390117</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000139">
        <property type="protein sequence ID" value="CAD8205061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XUH4"/>
    <dbReference type="OrthoDB" id="312185at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="9"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8205061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="443" mass="52035" checksum="4EA04F3BB12CDE8C" modified="2022-10-12" version="1">MKQIGHYTYDEINCLGQGAYGKVYEGLNTLNNETVAIKKLDLILFEKDKYLRKQIVQEIEIMSKLNHKNIVKFVELLATKKSLFIVTEICHNGDLKSLIASKNISEQQAIDIMLQILEGFKELIKSGVIHRDMKPANVLNDKGTVKIADFGFAKYVENYSSQLLKSCVGSPLYMAPQVLERHHYSTKCDIWSLGVIFYEMLHFDVPWKGRDEEDLLHNIKTQPLQFKNDLSTFSKQFLTITLVIEEENRASWNQIFELSNNSELYKSISSEDISEESIQKLPTWIQRPSIQQKQDKKVDEQKLQQIEELQQLRKQLAYQHFIMTECYTEQIINENNDCINILINYLNENIVKKSVYLIQTSIDLYQRFSQKNLLWKMQEQLNNEHNYYLQMQIDFKQQLSQNQCFDKPNDKQINEAKNQLLKCNNTNAAQMLLNLIDNYNWIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XVE0</accession>
    <name>A0A8S1XVE0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nucleoporin Nup54 alpha-helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1390103</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000139">
        <property type="protein sequence ID" value="CAD8205033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XVE0"/>
    <dbReference type="OrthoDB" id="303152at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8205033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="367" mass="41837" checksum="4C900654A93A5C6A" modified="2022-10-12" version="1">MNTNGPQQGLFGNKPPQTNTIFSTTPAVPQQNNLFANTQPTQQNNMLFGQTNNQPQTMTGGFGQTPLLGQPVQNQGIFGQQQPTTSNLFGNTQPGFGQQTNLFATTPQQQQPGIMQNGLQYPQQNQIQAFINDSNAFKEKYNALTLKIGETEKSFANQPGQNQQWQQTIQPISQIVFQMDTKSVLDTSEQILAGQKDLSKNLNEYISTVGLVQKITEDGLEVLKKEEAQCNDYQKAQQTMKESYDQLSGTIRINQQFSVPQKYQEQLILQQYEQLELLTNTVNNMIKYYEKQPNMDVDEALQYKLMHLQTLSNYQCMLSSRIRNIQEKISTFQTKTYSQIMPDKKIDEFYTIDTHKDLLDQIIAEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XX39</accession>
    <name>A0A8S1XX39_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1420014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000142">
        <property type="protein sequence ID" value="CAD8205820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XX39"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035885142" evidence="1">
        <location>
            <begin position="24"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8205820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="253" mass="28953" checksum="73477CD69F9505F2" modified="2022-10-12" version="1">MAHVISFLKILFLKSIIYENCITDNMADCRYTDFSIVGINSATLERCGSQPQNLFMGPFGFQTSVTKAFTNIPPNKELEFSVNIWKFDSWDSEGFEIYANNVLIKNLILGNEAGTQICRNNIFSDFLYTFSQKMILTGTDLTIKLKDNLNSASYYEDLLDESWGFRDFILRLSVPCVNFYDECNYTGTLFQICQGEQSKLQNYIPFEIKSILMGPGIIVKLKDKNYFAGVLQEFTTSQSCLNGYKFPKVLYSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XX55</accession>
    <name>A0A8S1XX55_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1410074</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000141">
        <property type="protein sequence ID" value="CAD8205627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XX55"/>
    <dbReference type="OrthoDB" id="291864at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8205627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="210" mass="25059" checksum="180140F57393A940" modified="2022-10-12" version="1">MFDNLPRTPYMKTRVQPRVLYNGFENTKILKVQNTFEYYHYLQKKKEYSDQQKELLEGLKMENDDMKRLSYSVKCFGVSNKEGKFGVQSQRKFMGGSFESHPTYDVNIQSERISYPFKSKRLMTSRRQLRDKQKPLSEIIKLPERPETVRTEHVSNVDKIVRKSRLKFKQQHSQPQPEIVFEKGKTKYQSYSRLLSEAHSHESFYKILSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XX95</accession>
    <name>A0A8S1XX95_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1410078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000141">
        <property type="protein sequence ID" value="CAD8205635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XX95"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="439"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8205635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1194" mass="143185" checksum="23A67A15147CBE7C" modified="2022-10-12" version="1">MNSTIKLKVDLNKVDQKQSILEIVEKLQGEIYLEYDGHIQNALIQTQNLIIDPNNIFHLNNHNQLQQDQYFNEAIGISKKIKFIVIHNLMKNHFYQKAKLLQMKIFPDWNLSEYKTIQQLNPTQQHLLEQINQQINKIILFSAFSKNSIEKNSKINFLYKSLDKLAAILETNQGNLLLGEVQEPYEYARYLNKLIFIINLNENVSEFNQNLMILQKQILLNRFINDNFKIHTFIISITKPKCSRIELKEIAYELIKQNQNINIKVGYNRFDITSFEKLIYFEIEQYDDEQNIEQFLQQEIQDRASKILRLNIQDKINFENFICKLPFITNSNIKFKHQKIGIKLKNSFYQDNQFRYQVNQQLKQLKSQLLHLKIDKNFSVYLNLVEIEQKKQILTNLFMDVTQQKYILYINDVKQQYIELIVYFHQFIKNDLITLEKILNETMDQLQIASLQMNELNQLELTIEQFQQKYSALLYIENQKLNFILHSKYLNQIKNLLNSYKTLTLLSYKPKSKLYAQQILYDYKNKIIEQSQEILSIKLSSNNEILYFECPTKNMTVLDIFIKYEQVLDQLLLEIPLQISKLESKYLHNNCQKEIQKISQEQIFDLHFNFQEYQTTKNSQEIKGKNINTKNQFIKFLTKEVNFDTNLKCEISMRMILNNNFDIQISYQQEDKVHPNVLSIKEYFLFYQKIKIPYKQIGVVYEEIKDIFPKLLQLFMSKDQTKFQELKIFINNDKFKSQYEQELLMTLITSIQETYQDFQWQWSDGTKFNDYDDAMINQQIEIAYQAYRLDNKKNEMILKFPYSYQPGTHTIDLNKGTILDHASALIQQIKQKDGYYYIGKEQADDILNQYIQERIQMKKQQFTVFLKKYLIFFKTKDEIYQINLDSKYKRKIRREIQTDKYFKFIHDFIQQKCNQETPQQTPDADNQIYCYAKTFIKQNQDIQQAKFKKTEEITNKINIILQQYIATYEIKLPVTNDQIYFSFLDYLKNNTLQIEGDLNQNETIYIKSFEKNLKSIQEVVQFLHQIPQTWNTSSYQRLYYFDILQPDFEEVKQSFPFLNIKTVQLIQNYDLWAKYQSIKSKLEGWKEELLIFGNKQDCAQEEIGKVTLSLSFDIHKGPYIKCGNTIAYVNDNYFESQKGQKQIILVQVLLGQPQDLKQQFVISDYAENNYKEGEYYYIKRNKIYPKFLITLNEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XXR8</accession>
    <name>A0A8S1XXR8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1430078</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XXR8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8206196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="146" mass="17063" checksum="E4CAC680F10EC2EF" modified="2022-10-12" version="1">MLNLYNSSFENNHQFQFQLKRYRPSYTIQSEPKNQLTRQHTIQDLLNLKLSGNIQSSQPVIQRNLLMRPSLKLAKITPSITESTDNYSSIKKRTISLENSKKVDFHPSVLVIYSENGIKKREKLIDECKIKRTGTRKNCIILIKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y016</accession>
    <name>A0A8S1Y016_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1420095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000142">
        <property type="protein sequence ID" value="CAD8205978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y016"/>
    <dbReference type="OrthoDB" id="303349at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="187"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8205978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="522" mass="62724" checksum="698BDEB843A3E07A" modified="2022-10-12" version="1">MNFSLPDALVLIKEGRANEAVDQLNKFIECLQPLSPQTQLLGQQLGIDCDFFRIVKFQDFYDPDPQVQALRFKYYAYKVAKWGYQIIQQKRKVKRRHDLIEKSEQFGIQNEIGRTFGVDMESKQLIESRLQSISEKLQKKKQIFQKQLLCQLDIEKKRTDYKIQQEDKIEKVRKALKTDYDNKIVAVKDHLEKVNEKLKNNNKMKKKMEDELINKRGELLKNASLIDEKINKLEEMRKEQIEHMKQARSIKKQRQHSFQEKQNQKYEEYLERMKQKLDQKRAVEFHSENHKDFGKRQVYDNYEKNLENIILKYQKSQPIINNLLAKSIEQVQQKKQIEIEKFQKVKSNQKLHLSTPRIEPRKVEDVLERKEKKLQVETILRRETYQQRVLNFLQNRQQLQRLQSQQIERYNEFYTRQSARLKNMRNSHEQMKSTVNFALMQANKIEEQTALKISRAIQLINDPDLQMQFKPNEKLSTQQKVAKKKQEEMQQLLKSFVTSEVDLLRFSKSVTEVKNEENQQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y149</accession>
    <name>A0A8S1Y149_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T1430051</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y149"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="68"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="381"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="422"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="14"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8206142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="520" mass="60582" checksum="DBB2097120EA8A5F" modified="2022-10-12" version="1">MGSCSGSVQNKQKQLRDERIRTEPDKERGSDAFTGGINISNVSRKSDELKGLDLPNQKKKTMKKQESLKVTLSITNGPISKYYVRKNLRYLEGVNFKDNMFVVQHVVTGKIRVAERFQINTNTSAFINKIVYLQLNHPNLLDIYGIFQEDNYYTIISDYCNGGSLLTLLSERNSHFEEEVAAQCCRQIYELMGYLHKFGLYHGQLSLKSFEKYNQGKKKFTIKLVDFSCLFCKSDINENNVQFLSPECCNENSSYTSARDQWAVGIITMAITLGKLPFNAKSQQECLDNIQQSCKKEGFIQKELEKQVANPLIKDLILQFLQVSPSKRIDFFQALESKWMKNYEQIFKQQFKQCLDELTKKKEANFLQSCFLLMMIKQFDDQYSQIQEIFNGLDLDKNGRLTKPELVQHYTQYFHLKYNQHEIQEFVDEIFDASDLNHSGDIEFSEFLIALSNQKTLITNENLKCIFNQINHGQPFGFIELQPLFRTKEAELQNSLQQIPNQIIDFEEFTKMMFELIENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y2A7</accession>
    <name>A0A8S1Y2A7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1450051</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000145">
        <property type="protein sequence ID" value="CAD8206778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y2A7"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="40"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="76"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8206778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="285" mass="33898" checksum="C3A55C96C7B4C4F4" modified="2022-10-12" version="1">MQILPPAKRVLHFCESKNWFNSRHANKTKEKYLKTEKELFDDIIVTETFHLIDRDHSNSIELDELYSMLKKNNYPVNMGLLKAFFEKTDRDGNKCIDLDEFKQVIKDESISQTFRMMMRKMREIGEDNYYSTDFVNLLRYLCYCSNRTDLIYQIKNTRLSYETRSKLVSDLFKVNQQFTKSLHTTQEQLFTLRPFMKKKNQIEFKYLTPIQKRPNQFSNHSTTISNQNSIISLQTINYQLQSHRSSPEGYLIKKKQTNLIPTTLQSTKSTLQSTQTTLKKNNNKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y4D4</accession>
    <name>A0A8S1Y4D4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1500012</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000150">
        <property type="protein sequence ID" value="CAD8208197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y4D4"/>
    <dbReference type="OrthoDB" id="309108at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF263">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8208197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="393" mass="47022" checksum="04830EE94F613C1F" modified="2022-10-12" version="1">MSGVEQQIILRNSESEERLQGLQNVPQNREQFLTKLKQKKEKYDQMSSIEFLSEEFKWKEDSRKLISSYIDNYLKDKPNCIIRQQSNCLPCLFKRSSEIGGFKKSWSKCVEQEYEKRFYGNIYFDNQDLQFMISQTLKVQDFEQLEEFQQRINQIKLGEIIDHFQNAKLFQNIFQFYDNDEINLIINYNRKYGSQLNYQYEDMNSDYLYGKRCEYGFEITKKENVNYIYIGQFFNNKYHGKGVLLQEPFQDSEGEEFFAGKKEKELFYFLHGIQKEGQIVVKQEQDIGYYHGEMKDNKRNGWGILKLKNGNQYKGQWQDNKMHGMGHFTWGNGEEYLGEYFQGIKHGFGRYKYQQNKIYLGQFKDGKQHGLALVQNSSKLNLYTEWRNGQLVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y871</accession>
    <name>A0A8S1Y871_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Chitin-binding type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1560007</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000156">
        <property type="protein sequence ID" value="CAD8209740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1224"/>
            <end position="1248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1254"/>
            <end position="1271"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="5">
        <location>
            <begin position="924"/>
            <end position="983"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8209740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1293" mass="147507" checksum="0B486BF075E9B464" modified="2022-10-12" version="1">MQILTQEMDGKILSVMLISILMEAFNILGNLAVAFLQLDEYFQIQNLILILFWMQNFLRNFILYYKSINADSLPYFYLDEQQMSFQPEISSFYLCGDTQLENKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKCLICQEGWIQDEFPENCHPICGDGIIQGQEECDDGNLISNDSCYLCKYSCIQFCSICQFGICLQCQDGFIINAKFNCDPLCGDGNLIPYSTEQCELTVNWGQDNCFECRFIPIEYCKTSYFSFCLECEVGFYIYENKCFPYCGDKLILEQYEDCDENNQQPNDGCFNCKFQCIEDCNICTGGQCIQKCEDGYEFVNKSCLSVCGDQIITIEEDCDDGNLQPYDGCFYCKYSCPENCFDCYQGTCLERNYQYQLLISNQCKKQLTCGDGYLQVQEDCDDGNYQAADGCKDCLIEQNWICSTITRDSPSLCSFVKAPNLIINYINMTQNKQYISIQFDQQVKIQTTQPLSETIEFEIKNIKKKNWISSLFIIQDVGSEVNFGGFVVYIEIQQLLQFRPILKIKVKQTNANIYDAVLNNVEKSITLQYPKFLDETQKEYSYNLKNLNKYIIYSLSGVTGLSLLLGSGDLFVEILAILQFQLYLRYINLQYPENIEIYFTFNDMITIQPLLKFIYFPQLLQLINIQLNQEYSEGKFNVYKQNTNLIINLSCQIFQFLMFVFFILCFQWIKRFCINGYSVLGTFAVCHLYHYIQIQKQSLSLVNPFITFAWNYLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8C5</accession>
    <name>A0A8S1Y8C5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1580104</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000158">
        <property type="protein sequence ID" value="CAD8210295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y8C5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8210295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="249" mass="29218" checksum="912A9693DD6A4258" modified="2022-10-12" version="1">MQKTQEKILDENNTCTYQFCESKAKALEKQEKWQEAVQCWDEGIAKLKRAPFFQAKKQKHYKIQENGKKLLNVGIKKSIKTRIIEFFMFGKVILKQPNSIGFKDQSLSQNIFFKGLLNKRQIVFNQTTISIFFCYLVCQNMMQEAIDCWNQGILNNPNDRGYYEEKAMALLNQRRNLEVIKCSDEVNAIFQESEAINFIRIYALLKCKGVITFQKLQNEFLLDNLKLLKKAIIARRLAKQDTTFQKSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YB06</accession>
    <name>A0A8S1YB06_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAD(P)/FAD-dependent oxidoreductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1570053</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the carotenoid/retinoid oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000157">
        <property type="protein sequence ID" value="CAD8209987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YB06"/>
    <dbReference type="OrthoDB" id="7777654at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10668">
        <property type="entry name" value="PHYTOENE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10668:SF103">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8209987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="170" mass="19676" checksum="E88375DCEBB49884" modified="2022-10-12" version="1">MKKYDAIIIGGGHNALVAACYFKYKNILLLERRHITGGAAQTEEIYPGFKFSRFSYLLSLMRPSVINELNLYQHGLKLFRRPISSITVTKNKGEYLLMNSDSTFCKQEIAKFSKRDSEVYDEYNQFLSEIIDLWSYYQDKYPYDTSAKGNGYNIKKSFDDLGLLLKIGQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YD05</accession>
    <name>A0A8S1YD05_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1670102</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000167">
        <property type="protein sequence ID" value="CAD8212356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="77931at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035850898" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="2763"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2315"/>
            <end position="2333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2374"/>
            <end position="2395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2426"/>
            <end position="2447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2459"/>
            <end position="2488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2508"/>
            <end position="2534"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2573"/>
            <end position="2600"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2649"/>
            <end position="2675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8212356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2763" mass="321522" checksum="040FC119A6FC96F0" modified="2022-10-12" version="1">MVRGTSLSLIIIIALRITFEAVFKLQPNYPINIFEIKPNPIEIYNTYFGIWFQYLPLSNTTQIGNIGILDSNCFHLLHTKEQKTERINFLLYLCLDYEIKTVFTYLQFIDNTGLDHFHKIQINEEFLESRWQYIGFCAIPAQSRFNIFYQFSGELTKQFEVLVKFPFYDVYLNLIYGGGLIVNDNQKLQTQINKKLSYFPGLILRDEYKCEISVCKLSYTKMPGYEINGCYCSQNKNSTIIDSIIDKQQEFYYLSEYNNCQEFLLSGWIKIVETKIFEDEYYYQLIKLSGNFINPQLIDDNLSAFTLTYKIATNGNQIIVTTYSYVFPLINIDFSKNPNLIKNIFDIGSDIKSWHYLLVWKQEAIIYISITFYEQFNIEKLEFNQEVKQFGSVKFQLHIGNKLQVSQQYLTIKIQGFKFYNCPKTINQIKNCHPTCKECDGPTKYDCLSCLESSNRIYVPDSKQCICKYGTMDQDNFCISHESLNIIILPDESKNNKCKYGYYEQDSACQRCPSTITDNVITCLECLYNMNDWINKFCCLTSLYTDNEGNIYQKKEEMVCYNSPPPDESQLDICLVGCDIQETNVSPNPSTGLTILHLYCSAPNFINFEQKCTPCPIKYCQYCFSYFANDQAKTTLDQNFNYLIFEEDIRTGCAQCQNGFIYNFELELCIFKSPSNPNCARSYLQQDGQEICTLSKLDDFSIAPTIINCQQRILNCKQCFQTPELILKCQVCEDGYTAQSGSGICSQCNNQNSKICLELNSKVQEPWKWFIQDFRIKFQNLTIFDKTYVERTAIFVMECKDGYQVIDTNCYKYCDDNCQICKKNFEDWNFFSCQKCDSNYYQIPNRVQIDGKCIQCPPLCQICELRSIEEIKQINPYFILTPENTIFSYKCLKKIPLPRATIDQYSQFAYYCFNDNCNQLFEYQVNQSCQSYSLVDFNDIQHYSYFNNIGLKEITLIFHFHLFCPIMQKENFVISNYFKEKVFSIQQVKLRIEGEFKPIIFDTQFKIQNFDSISIKNVKFLLEKQFELIISNRAQSFDFMITNTSFYSNQSTPIILQIYGGSIQNFQLSNIDIFDINFKNSIIIQIKSEDLGDSIKINNLQISNCKLNSTTLLFFSNLNREIIIDNLVINSCIFNASSVIQSNISFSNFAKIIINNVNIQNSSFTASSFIYSNGNSDVKITKLLLFHCQIMASRFISFSKNFLFSDILLEDNLIQETQFLIQLSSNDKNSEIGIQNFIVLKNKIFSSALIVTEQSFEQNQVYFKFNNFRFEDNIPISQENYQYLFQINCHNLLIKDFILKSTFNFKFFSLFSTPYLRFENIIYENQYQKEKVAFKFDCYQSSAIKSQLLSVQGFQRISLEQIKIINQFSTDLSIISIQSNSKIILNKIEYISISNVSFIGNILIKYSQTMYFSLIDIYSEITQIVTLENIVYQENIFHQYQIDPSQNSASLMQINAGQSKISLKNISCSHNVLTNSSNSFISISTFELYIQDFQTVNHNYLNSELWNQYYLLQIQKEFNQIQIISIIQNTFKIESISGALSIQASKLSFINGFFSNIIASSSLIFNINLQGDGIVIIKNCVIENAQSQLINTSQADGAITINAQKSLLSVEIENLNLRYIQNRISSSIFSIYPSSIKNNIQLKNIYAYNCFSLSNQLISIEFNFNNPQKSQVSIQNLHLIQSEKKFINYLQSLGGVTLVEIQNILENNAIIHLTGGFFSLKGIVIEGLILSSIIKIVDYKQITISDSQFFNIQTFYSLHLLEFSQINVRQSILIMNNITIRNVNTFQVNKYQLNYYRNNLNITYTNQCTLIIDFVKIPTEGLESINIFFEQMQLNSQQKGSLIYFSSFKNQTQMLIRRFLIENNDCQNCWNGLMFFNLTEIKFIKINEIACIRNLIKKHGCILAISDNNLDRQIKMQNLNFMLNNGTKGTGVQVQNLRISILNSRIINNFAFEKGGGIYFNVNSNRFLLNQTLICQNLAQEGGGIFLQGNSSLNQINFFKSLNLYNFATISSNNINELPSHLGLQINQKEMHSMEYVIDNQSIYILALKPYNFLSQNHIKRTNYLILPSGQKVSNFKLFNPQIKEYITFITDISIIFKNSMNEIQQNFLNSICNVTQNVLDIQSQQILKTNQFQSISFSAETNSFSLDQIEFNFDPYSQNDKIYEIMIGCKTEYLEKIFFYKMRVNIFLCQLGEFYIQDICLKCQSDQGFYSVTYNATKCSIFDKNKFEAITSNQIKLKSGYWRPKYISDNIELCYKNTNQCLGGWSVGDDTCNIGYIGGLCEECDKFNIKGDGQFFKNQQQQDCSQCQEFAKRLLAFFLIAIWAMFSTLLTIKSVEKSNLLFTQLKLGQKFADILFNLGQDHVSILLKLYLNYLWIFSLILTFNINFSFSLSIIKQSTDTSFFMANFFDCFISELEGIELIYSKIIVTICLLLCQIIIVQSASSLLQFLTSYKHRGRIISITVLNLYIQNYASLITSFFSILAVRNISQVSYIQGDVSLMYGTNNHYQWIFTFILPGLSLIGFILPFSLFIFLQSKKHQLNNFKYKRHFGHLYNEYTQNNYFWEWIRFWKKIIIIIVLINFDTQILLKASLFGFCLQVYQFFTEKYKPYLLNRFNSLDLKTSQLCSCAIFLAAIKYTCDQSYNYQISAILQTIILLISLILSYPFVSVILYAYYKRFEPDILKFLIAILHSYKRNSKSIKYLRNHLIQLGQKENNLKQNFMKLRSVVKQQIQQEQKKTIKIISNKQKNTNELDFQSKLGRKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YD73</accession>
    <name>A0A8S1YD73_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1630030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000163">
        <property type="protein sequence ID" value="CAD8211333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YD73"/>
    <dbReference type="OrthoDB" id="284847at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8211333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="185" mass="22017" checksum="DD77A082304F62F5" modified="2022-10-12" version="1">MEELGMRPETSKCSRMEKRRLVSQSHQRQESSQAPRINGITLQQYFRGSNFRVLEINEDEWDNHQNGQSLAPPLQNNNFRIKTDKKINNKKLLERSPVQNLTTSKDFNQRKINRPHITSNEFFQNEDELRKTKIELKYGRMKEDVINRFGNPDQDLINKYAEKFNINREPPKLTLAPRELYTKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YGS4</accession>
    <name>A0A8S1YGS4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Potassium channel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1620015</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000162">
        <property type="protein sequence ID" value="CAD8211052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YGS4"/>
    <dbReference type="OrthoDB" id="297496at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="353"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="398"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="427"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="3">
        <location>
            <begin position="371"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="494"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="795"/>
            <end position="822"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8211052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1054" mass="124016" checksum="14CACBF9C01C188A" modified="2022-10-12" version="1">MLNLQNAFDFEQDVELRAVQNDDFEQQYLGQCLRETKKEFTLILPQIASRTLSAKSLEAKYLHSPKSPESNNDENFLASNIQTPKEPIFNTKLVDRRRSQTNAESSFMSLFFIGRFVEKLSLNRKQLGSLNDSHFNLIGDKASDSQNIFMHSKRMKSAGFNIKKMQTLLKKETEIKDTTTLEQVKEDLRKLRKPLFNCLNAIINKIPIIQPESLFKMYWDFFTSIFRILLLILVPLEIAFKPEILFNNLICITYVILFILQLDFLIRINTLTYKNGYAIKDKWDLVVHQLKKEFLTDFSTSLILIIFMIIPDMNTNANLFLLIILGQHKYVYETFAKSDQISYLTRPQRGVFGLLKFILTLLYILHLFSCIWFYFSSIRVEDSWIRFNEIENKDWEEQYLQALYFAVVTMLTIGYGDMVPKNAIEKIVTIVFVLGACLWVSYSVNFIGSIIDDITQNQVERNRRMRVINKYMDQRKIPYSLQHQVKEYLTFRWKEDDEVDLEMEQTLLEQLSDELKEELDKQAHKVFIQKSEFLQKYFSEELRNALFKSIKRKIIPPQNTFSTDFLNEQHLCFVEQGFIVYQHPERKQRSKMNAVINQGQFFCVKDFITNNPQVDHFKAIGYVSLLILSKSDFMETLKDYPEDFQKYCQLKEAITLSYHPPALENGVFCPACLNFKHSLNTCPQVQYVPNREAVIKRHCMSNFQTQQPYKRRLDKSQIEWHTRLMKDLAQQYASVFQNENQQSIINQTRILLNVECGSDSNSSIDNDASSPAFKIPQTAPIFQAQHIRSSRVNRLVSDQNNKNEQQNNKNELRQNLNNALQYRKQSILSGALGLSKSPKNRKDQKPIPLVDEIKEYDSGFQNNSSDENEMNTSIDFKKKRDQIVEAIQDFNDNMLDNITNLYHQLQRNLEMNENDNISKKALSQLEPLYWSFNQQKFDDFEIKASFDQYFNKQNAEDVIVYVKQDVFGWQTSILQKMRKFMLYPFLYVQNFLRSRRNAKMKSITPMKVVAKVKSTLDHLKKTLRLKKKTTFIRSTTKLNSVVPAYVNPFQQYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YIZ6</accession>
    <name>A0A8S1YIZ6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1620057</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000162">
        <property type="protein sequence ID" value="CAD8211134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YIZ6"/>
    <dbReference type="OrthoDB" id="308572at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8211134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="336" mass="39590" checksum="8B95DFD8DD1BBA37" modified="2022-10-12" version="1">MNICQDSKINCQLTDLKTESILNDLEQITFNNDSTIVITIQKSYIQAYFQKNEDLKSISSFNVLKDKYYSQLQYLTFSNSFIAIPKSQILISSLNGLNARKFILKIDHGLGLCYKLLLNKNENILFLSYFNEIRIFQKQIIGWKCIDTLQSLSHRDTIQQLCLNFEEDHLVIHTSNGYSGSPTILVYRKKSIEQQIQWVIKQRISVENGMICGFINNNQFLFSDNYSLQFYTQNKDNQKFESENYYNFILSNYQFKSFAFQHQNQVLFIITKNELSVIQLQEQQKLKQRQLIQIDLSKLLEEDVSTVAFNSILTKDGKYLYIWNRKKTKIIRILNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YL61</accession>
    <name>A0A8S1YL61_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1750029</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000175">
        <property type="protein sequence ID" value="CAD8213207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YL61"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="76" mass="9049" checksum="809DDED8CE7361B8" modified="2022-10-12" version="1">MLHISYICVVDSHKQLNWGFKKNSRSMFSLLKGRQKTKMLCLEGIIYQLDLINHKQDSKIWLECIFWNFKANGDQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YME4</accession>
    <name>A0A8S1YME4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1670051</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000167">
        <property type="protein sequence ID" value="CAD8212254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YME4"/>
    <dbReference type="OrthoDB" id="306707at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8212254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="323" mass="38098" checksum="EFB32233C7EF3F3C" modified="2022-10-12" version="1">MTSCIQSVFKESPVLQHIKIIQTHHHIPNPYILQIQIICPSLSTIWIQFPWIGSNLPDQSSSETSQNSQFNDNESCCCFNCGKVNKKVRSLQKKFIAFHTQMSVLRKFRLLKRFQNAVYCIIYLIDKKRKSKKNQKQKKMYRFKTKINFDASPLMPPQQQQVQYEKHDDKQNQKNQQLQSIQAKIIDSKHKQRQSIRVYLQQKLNEKDHQQTLPKIHKITTNSFTTIQPLRFSKNEEPKSSRFSSQIKITPQTEFSPTSLHNHSPYFRNITSRNHIDSIIQTSFIKTPRTIIKSISSEQLIQKQQQHQFHSRKNTPSTFKGIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YMV9</accession>
    <name>A0A8S1YMV9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4-alpha-hydroxy-tetrahydropterin dehydratase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1680049</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000168">
        <property type="protein sequence ID" value="CAD8212444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YMV9"/>
    <dbReference type="OrthoDB" id="277398at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008124">
        <property type="term" value="F:4-alpha-hydroxytetrahydrobiopterin dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00488">
        <property type="entry name" value="PCD_DCoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001533">
        <property type="entry name" value="Pterin_deHydtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599:SF0">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01329">
        <property type="entry name" value="Pterin_4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00030497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8212444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="175" mass="20587" checksum="F5584C93245B0BA0" modified="2022-10-12" version="1">MYRKLASNNNQAIANLLELKFLLRDFSEISQKQFTKLNQDEVNQLLIIQQLNQNWNQNVNSLSRKYQFLRFQDSFSFMSQVSQIADQMKHYPKWFNKNGLVQMDLMSPEVQGVTFKDVILAHSAEQISQVIVSQPKTSIFDNCDIHIENLISSWNNNYQKFQGLNQVFERSVNFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YN13</accession>
    <name>A0A8S1YN13_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pentapeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T2640001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000264">
        <property type="protein sequence ID" value="CAD8214871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="311341at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8214871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="90" mass="9942" checksum="B193B487A96AA230" modified="2022-10-12" version="1" fragment="single">MQVDIRNQSLENIRIKKTSVFGGNFVRCNFSGSEFDNVDISGLNLNGAQLFNSKWKNLKIFELNKLDGHSDFVNAVCFSPDGNTLASGSX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YNK5</accession>
    <name>A0A8S1YNK5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1960005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000196">
        <property type="protein sequence ID" value="CAD8214097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YNK5"/>
    <dbReference type="OrthoDB" id="309452at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="135"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8214097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="355" mass="42231" checksum="BD073EB1D9DB9916" modified="2022-10-12" version="1">MLTNNQYTQLPVQSVLYQQQPQTNQRLPIQQFQSPVQMKLISPQPTMVYQQQAAQSQLYTQSNQHVKTIQQHHTHHTHEYKIPQYQQPIVTAPQQPIIAAPSPPQQPEINEMYHVERELQVLSVEDVEEPWKKKCVELEIKIFDLQTELARFKNQGQELKVTSNEEFQVRELEAKIKMMKDIEDALRKEINNQQSEIDSWRQRYSKLQLEFQQLLQGGEEVRQLRDALAEKERQILKLENALNSQNVEMQNYMRLVQNKDQEIAQYKQMIRQLEQEMSEYQQTTEKLKYYSNEADIWKERFMKANQDFHTAQEQWMMAQAELDSLKKQKTMTTTEKITTIQQTNTRSSKAGARQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YPQ0</accession>
    <name>A0A8S1YPQ0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1840011</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000184">
        <property type="protein sequence ID" value="CAD8213692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YPQ0"/>
    <dbReference type="OrthoDB" id="298777at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="708" mass="82333" checksum="D1810CB22DD18474" modified="2022-10-12" version="1">MFNKISYLQKKLQITFNGQYKNTKKDGKWDIFYEFPKQQIGGGLYGNGVKIGKWIEVDERSVLNYIVTYSGEYRNDKKVGLWTTLNKQSGIEQLCGSANYDDDGTEIFKNDEKSKILYIGEFRNGKKVGRWDILSWDSKNANFEQIGGGLYDEQGSGNKIGNWIEFKKGYKNNSQITFNGEYNNGKKVGLWKTYKKRKNLKIYNQIFQLCGCSNYDENGIELYTSDKQKLFLVVIRNLNIFYLGEFMNGKKIGRWGIYNRWNKKQDFYKIGGGSYDEKGDGNKHGKWIELSNQFGFYRNLIYWGEYKNGIKVGGWDIGYKGKLEKVYKKIGGGTYANGMKIGKWIELNEQYQEKTKSQYYQCHDVFEIYQGNYKFGKKFGLWETFKNQNQIINFYILSGSANYDEDGTEIFKNDEKSNFLKLGEFRNGKKVGRWIIFFRPYNNDKFQLIGGGFYDTECEGCKIGNWIELSDECQKSTNSTKYQICQGDYKFYKKIGLWKKFNVIDKNVQLCGSANYDENGTEIFQNDEASKILYIGEFRNRKKVGRWDILFGRSFEEFIKIGGGQYDEDGNFKIGQWIDLIDSFGQNRQVIYKGEYQCGEKVGKWNIVYVPVVGKTHNIIGGGQYDNSIKIGKWTDLSDSFTSNNAIMYKGEYKNGNKVGLWDILFDWIGGGFYDKEGYSIKIGQWMELSEQFTKFSQIMTIGEYKNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YQ76</accession>
    <name>A0A8S1YQ76_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">H-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1960016</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000196">
        <property type="protein sequence ID" value="CAD8214112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YQ76"/>
    <dbReference type="OrthoDB" id="304609at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019019">
        <property type="entry name" value="H-type_lectin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09458">
        <property type="entry name" value="H_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035945592" description="H-type lectin domain-containing protein" evidence="1">
        <location>
            <begin position="16"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="H-type lectin" evidence="2">
        <location>
            <begin position="51"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8214112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="420" mass="48429" checksum="078C4C01D19D2C87" modified="2022-10-12" version="1">MFIFHIIFSIISVYADHLFESGEVSHFNYASNPNLILYTTTTKFRQEELFIPFAKQFSSVPDVYLNIMLMDFEHTFPQGYSLDITSISTMGFTVKVVCESTEQYYGFHFNWFAFNDESVQVINNLNITYPNSQYTHSYNKNCKINVALSNFVSYYAVGPQFNNLTGLSLTSESVTISFYNENLKQFGYQILLSSSDFILIGPTITDISLYGSSQIVSFPSGWDTQNCYPQLLGLKHDGTAQNLRLSGSVTYSPGVTVGFYPWFTSIIQSLQYNYFCINDAYFDLAVFGGLVQTKFFETKHQIETHIEISEINYYQNQNIEEEITIQNGIQFIQIIYYWKCLGYEQLKLQLFCPEVWCSSIQVQCNINKTNTIRLKAKFSFTISSQQYINIIKTSTSFIATQIIKQQNPFQKVLFKAEMVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RRG8</accession>
    <name>A0A8S1RRG8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0010048</name>
        <name evidence="2" type="ORF">PPENT_87.1.T0010267</name>
        <name evidence="3" type="ORF">PPENT_87.1.T0470058</name>
        <name evidence="4" type="ORF">PPENT_87.1.T0530231</name>
        <name evidence="5" type="ORF">PPENT_87.1.T0800049</name>
    </gene>
    <organism evidence="1 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000047">
        <property type="protein sequence ID" value="CAD8167318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RRG8"/>
    <dbReference type="OrthoDB" id="307391at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8131451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8131750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8167318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8170758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8183041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="120" mass="14504" checksum="BCE961C2B31E6798" modified="2022-10-12" version="1">MPIYNEVWEEEDFMFRNMINLQTLTKNHVKLLDNLKFEFVEYKANQLLACHLYDRMAQHCKNQFGLFEDSYVPECLDARNYFQLCVRMNASYGLAKKYFPEYFLTNEYSRPNPNFKELGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RXE5</accession>
    <name>A0A8S1RXE5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPENT_87.1.T0010443</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:46609"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RXE5"/>
    <dbReference type="OrthoDB" id="341578at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004694">
        <property type="term" value="F:eukaryotic translation initiation factor 2alpha kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF160">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0652">Protein synthesis inhibitor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="222"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="86"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="86"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8132102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="587" mass="69859" checksum="1BF74D24D1D2BD76" modified="2022-10-12" version="1">MIKKQAISKQQQIYVGTQIVEFSWFKQHEIVYEQPTNQWKQLIFDNFEWFLILLLLIYCITFYFIYKAFSKKANIRKESVASDDMQNSVRLSSEQNELHKNDTTSSTESSPRQKIFTQTQDSVQEIKQINKRKLTDISEEKEQQKKWEKNETDNNIVHREKNKDINKLEDNDQFIKSDENHSNLKASHNIEQDCAKEQIQRIQLRLPDWSQYLENGKFKKLYASPTIIGKGSFGEVYKCQKIVDLKEYAVKRIYFKVQNEKNLRDHPIFREINGLQEINHKNIVRFYTSWIEELSAEMIAEKRKLHDLVNEKQQEIQNNVSIDSFHPHIIDDMSYLNDCVQFIGEGGTENDQQLQTISKNSQTIRSLHLSDFNSQVQSCMRKFHFNPNQKDEQYQLFLLYIEMELCDQTLKDFIEYVDRKKDQIKIKTYFKQIIEGVIYMHNQKFIHRDLKPQNIFINSREEVKIGDLGLCNNPILQDEKELVQTNFEYTNNIGTPMYMAPEVKGDQYGYAADIYALGIILFEMLWEIKTYQEKFKLIQNLTKDYKLPPEFSKDYPNESKLILRMVSRNPNNRPTALEIFETLNKFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S188</accession>
    <name>A0A8S1S188_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0030087</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S188"/>
    <dbReference type="OrthoDB" id="292715at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050626">
        <property type="entry name" value="Peptidase_M16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690:SF18">
        <property type="entry name" value="INSULIN-DEGRADING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690">
        <property type="entry name" value="NARDILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="144"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="9"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8134008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1074" mass="125364" checksum="BCFBCD5A447B519B" modified="2022-10-12" version="1">MSDEEDEERLLKDQDSREKQEYTNQKKGDQNKNKTFDDQSGPSKFDAIVILILVIMIGLSGFGLLKHYNVLIPSQPIDNDVRGVLSNIHQKDSAVQEVFNKISDGLQNVGDSKAPKEDELAIDSVLDSYISKIEQYTDVQTDWVDDQDEINKKIEEQLEDQQNIVQDLEELIIQQESEIQILKPVFSKNIYTYKELQNGIKVLFIQTTTEGIHMTITLNIGKKNDPQDKPGLTELLFRCLQTNFYIEIYNHYTTLTAQLNLFEELNTKLQELYNILIKPQFIDIEKNAKDLFDDLMHDSKFGDLEYVSEFLSQQTQLKELNILEVDQEQLYQQYIQHISADTLTLVFKTQDESESILDKLFQSDLTKLKNLHRFQQPIFQNRYPLGRHILKFKSDTEVLTILSMSDQYSCQRFLAYLLPKPFYGAMWQNHLLVTLDIDLGNILISINKLIQFLDYLKHADEIQLEHLYSQMLATEELLFNTKYEENLVKIGQNLFSDPIYALKGQESEPIFDKEEFKQYVSNLANNFVILVGSENFQYNQTYESQKDDSIFILDAVLNKVHQGFTYDLKPLVKLFELNNNYQFQLPSISPFLPENLAILSVCEPVYKIQGFGYQKVDDLSLTVKDGKFDGSREPKFETVEFVCEFPLPDFQVDCLEKEKAAQFSLTPFQIASNVWWKPSRLGAMVFTGLFIEKPESTLAQGKTMLKLLQKYYTELAKRLFQQEFLFGYELSFKETINGLNVQMLSYSEKQSEFQDKVFDLMSIDDEQLFYYVKDLLNKDIRRFHDQKLSLLTQQYYLPKIMLRPVNTPQEILNVLDEVDYQKFLEFQGSLLSSKIEILNIGNILPNDDAFEDSQETYMTRTLNLKGQNLKYIVQRESNNDMTSSVLNYYQTGLKNIETTSKLYFYADFLQSYSNNYFKMGQQVLIKRKPLGCADGLQVYMQGILPSEGNEEIEKFLKQANLHLSNLQDEDILEQKLYTINKLYNEIKFKTLQEESQFIWDKIVNKNYAFNEIQDVIRYLDDAFPQKLRECANLVMQGQMSVQVYAVDQSIENTEGIQSLEQLIDQDVYECFFTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S1I0</accession>
    <name>A0A8S1S1I0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0020402</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S1I0"/>
    <dbReference type="OrthoDB" id="301524at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="355"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="312"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8133432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="411" mass="49673" checksum="980B902C0321E8E5" modified="2022-10-12" version="1">MNNNQVFDELEEFVKQQKLNDNLRIEAKVGTIIAKTDEALIDLQHFQDQFINVESKIEKKNYRFESAISKLDYEKACQMLKSEKRWEFTIDIIFALSYTTNYSFRMGYDEKGKIFCYERKKELKSKHLDILDQKRQYRITLSQYDIIDYNQTEICLEKIREYLRENTLQISCIRYKQTMIYQFDDYCESFVFQVQQIEKSKQAFLIEIAKEITNKNNTFKKNLDNAMELLQQHKNYYKPFYEAEISIKQYKVAQSTQNLVEKYVKKIQQFHDDVRAQNPQPTQAGVRDAQFQKKYVKQDQKQRNYNPQKGTQIKTRGQNQQQRQDRNYQENQTLDYNNSNYLDEDTNKIENWQQNQPQQIKKYDNNSKTMYNQRGRGRGRGRGNISGYGKEREEWSNSDDEQFQSRQEDYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S5F6</accession>
    <name>A0A8S1S5F6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CCDC22 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0030186</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S5F6"/>
    <dbReference type="OrthoDB" id="303898at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097602">
        <property type="term" value="F:cullin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000060">
        <property type="term" value="P:positive regulation of ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008530">
        <property type="entry name" value="CCDC22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048349">
        <property type="entry name" value="CCDC22_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15668:SF4">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15668">
        <property type="entry name" value="JM1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21674">
        <property type="entry name" value="CCDC22_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCDC22 N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="238"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="460"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="567"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8134204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="596" mass="71045" checksum="DA39D613F8F37C4F" modified="2022-10-12" version="1">MEDQDQMLLKSLQLQGIQIQTQSLPQLTCEDLISIFLQFFQLSGTQAFEISSIKSIKSSFRRVGKIQEMLYPIGIRIDIQSIVNPNPQESRKIIVSLLSKLSQSNKKETGAKHLTFEERLQEEKANYRTNQIQNFFQEWVNPSLCAFPEVEQRFLRLHVLNIIKKEPLIKQVANKGVRKYLYHSIVRVMDHHLQKKEVDQPLQERLQLIKKMRRSDDPEAVLGQFKGQFQAEAEKNDWEDIQKGNRQLKRAIKQLKEEDKEEEQARKQKEDENQGLFNLEIQFQEEVKEIQQEIQDTQEAKSEISALLEDIQTKLIEQQKNYDKLQKKRQKLEDINQKQQDIYSQLQSDNKDKQQELESAHSILQQIESKKGNDPEIQQLEQEIENIRIEWEKEKILQQNDADELAQQVQDRKLKLEQIQDKMRRLQEENQMIRNQGRVNLEYKEKLLVDLQNQPKEIARNQYIKKIVDLKNQLEKQKTEYLKQAKELSQLEDSLQFQDNMINRYCIEIENLINQDPKKSETVVKQIQKQYQDYKIVYKQSAELMRNIGEKRIQIYDTELKMEDILLKGYKKNVEKLRQDLTDIQAENKNLEKQKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S9C1</accession>
    <name>A0A8S1S9C1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0050276</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S9C1"/>
    <dbReference type="OrthoDB" id="669224at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="78"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8136688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="610" mass="71272" checksum="CD76A2634BC0BB66" modified="2022-10-12" version="1">MHNLFQHKIQKSNNKYIKKPYQISYNKEQFICNQLVIGLDRITIIINAYSNQYFLFLLKNLLMFYREGNTYTNGEYTIYEQKQIGKGAYGIVFECTRKNRNDNLCAKIIKSMTNNSEYLSRESFIINTLREACPDNPNLIKVYYCQYQKFENNDVLIIIMEKCSSNLKDDLSQKQKYTSDEVLNILKQLLNGYKPLYEKSILHRDIKPENILISFDNSGKPIYKLGDFGVGKINSSNDTTITKVGTPVYAAPELNCLINDSSLDQAIKDLKLIQNGKSQVDVYSIGIMLYQFLFGEPPFQTQNPDQVKTFFQNLKHTPFKIKEPIKDINSDLLTLIEKMIVYNPKDRIKFSEIYSHKLISIQTQQPTKFDKRATNPFQNQNQNQNTFSNQIPFQNKIVFANQNQFQNSVPTQFSIQKQNLRATNPNPPQTQFNNPGPRMGTLNSVQIDNKQNQQINFQPPQNQIQPQIGQAQFTPQLVQSQFQPLKPQTQVQTQQNNQQIQIDELQDTIFYLQSQLSENYFFYSKQTIRLLKQAFDSVQKCDQSQINIYTDKLMECYKFQDKNAYSLGFLKFYYCLINKKNKLIIQRQFNNEQELIQAINNILKQCPPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SA90</accession>
    <name>A0A8S1SA90_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0050340</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SA90"/>
    <dbReference type="OrthoDB" id="6033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48038:SF2">
        <property type="entry name" value="OS02G0536400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48038">
        <property type="entry name" value="RIBONUCLEOPROTEIN RB97D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="11"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="83"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="83"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="122"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="145"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="170"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8136775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="183" mass="22228" checksum="969FFC1C43DC691D" modified="2022-10-12" version="1">MSRQQFRNARMSLFVGNISRLVEQKDIEREFKVYGDCTVDFRVKNNDRYAFIQYKSEQEAEAAKNALHQRDLQGLRINVEWSKKSGRYDENREPRRDRPRDDRGQSRGNYDRFEREREKEKKQRRRNSKSRSRSRSPKNRSSPQYRVSRSQSIDSENEPPRDKILRKKLKEMMRKTDNGEYKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SBQ0</accession>
    <name>A0A8S1SBQ0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial</fullName>
            <ecNumber evidence="20">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PPENT_87.1.T0050217</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="15 20">Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a quinone + succinate = fumarate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:40523"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="18">Flavinylated by SdhE, about 5% flavinylation occurs in the absence of SdhE.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 20">Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 20">Mitochondrion inner membrane</location>
            <topology evidence="1 20">Peripheral membrane protein</topology>
            <orientation evidence="1 20">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="20"/>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SBQ0"/>
    <dbReference type="OrthoDB" id="71672at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.700.10:FF:000001">
        <property type="entry name" value="Mitochondrial succinate dehydrogenase flavoprotein subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.80.40:FF:000002">
        <property type="entry name" value="Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000482">
        <property type="entry name" value="Succinate dehydrogenase complex, subunit A, flavoprotein (Fp)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.100:FF:000001">
        <property type="entry name" value="Succinate dehydrogenase flavoprotein subunit (SdhA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003952">
        <property type="entry name" value="FRD_SDH_FAD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011281">
        <property type="entry name" value="Succ_DH_flav_su_fwd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014006">
        <property type="entry name" value="Succ_Dhase_FrdA_Gneg"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01816">
        <property type="entry name" value="sdhA_forward"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01812">
        <property type="entry name" value="sdhA_frdA_Gneg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE 2 FLAVOPROTEIN SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632:SF51">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000171">
        <property type="entry name" value="SDHA_APRA_LASPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00504">
        <property type="entry name" value="FRD_SDH_FAD_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 20" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8 18 20" id="KW-0274">FAD</keyword>
    <keyword evidence="6 18 20" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11 20" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 20" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 20" id="KW-0813">Transport</keyword>
    <keyword evidence="5 20" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="FAD-dependent oxidoreductase 2 FAD-binding" evidence="21">
        <location>
            <begin position="50"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarate reductase/succinate dehydrogenase flavoprotein-like C-terminal" evidence="22">
        <location>
            <begin position="500"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="328"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="55"/>
            <end position="60"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="78"/>
            <end position="93"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="395"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="428"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="439"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="444"/>
            <end position="445"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Tele-8alpha-FAD histidine" evidence="19">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00059077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU362051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="CAD8136572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="636" mass="69929" checksum="D72E0A22FCBE6198" modified="2022-10-12" version="1">MFAITKQALFRQQAMKLMNRFAVVANNKPEKGPSQGHLTDKYTIIDHTYDAVVVGAGGAGLRAAFGLVELGFKTACISKLFPTRSHTVAAQGGINAALGNMTEDDWRWHAYDTIKGSDWLGDQDAISYMCKEAPKAVYELESYGLPFSRTPEGKIYQRAFGGQSLKFGTGGQAYRCCAVADRTGHAMLHTLFGRALGYDCIFFVEYFALDLMMDEQGACRGVVCMSMADGSIHRIRAGYTVIATGGYGRAFQSCTSAHTCTGDGGGMTIRAGLPMEDLEFVQFHPTGIYGSGCLMTEGCRGEGGILRNSLGERFMERYAPTAKDLASRDVVSRAMTKEILEGRGVGPEKDHIYLHLNHLPAELLHERLPGISEAAKIFAGVDVTKEPAPVLPTVHYNMGGVPTNYKTEVLNQVNGKDQIVPGLLAAGEAACASVHGANRLGANSLLDIVVFGRQAANLVGEKWKPGQKQPDLPKNAGEAAIARIDRLRHHEGSQTIAQVRKDLQRTMQKHAAVFRIEKTLQEGVEKVKEIYQRKDDVRIKDKGLVWNSDLIEGLELENLLLQGKMTIEGALNRKESRGAHARDDFPDRDDKNWMKHTLARIQDTKSGDVQLTYRDVITKTMDPNEFDTVPPKKRVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SCI0</accession>
    <name>A0A8S1SCI0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0060313</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SCI0"/>
    <dbReference type="OrthoDB" id="307560at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="414"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="384"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="414"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8137826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="684" mass="78205" checksum="310335AD73E8299C" modified="2022-10-12" version="1">MQQTHMQEEKQEINPDSSPSLSDSQNDKKEPIKQYFSTKCKWISFSVTFFVLAGIGIFLGIYLSQNTSSAPTLKKFQRDEFQQVDEICMHYTSDKIHNCTQIEMKTKRVVNDVNKQNATSLLMLTSLKVKTFQQNSDGTRQYDEMIDQNTEIEEQIQKSLRRLQQSQPTENDNFCEGIPADECGNVNEVPLVTVITDQQTGAIQQIGIPIEIPDLLIQPLVSNIIHIAPNVAESTNTTGTDGNRLLKEDYSNVYFIGKEAFIPKSSKTKSWFGNTVIKKTISQSDQIDGNSQGINIFDMFEQSQETILDNNNYLVSSHITTDSKYSNSIQTENVNQNSDDLADKSETEITNDSNLITVETKSDEDLTNLLTEIKNKQTIQYYTIQDLQDKIQNQQDQQDQVQDLQQEGNRLLENENGDVHSNSETEYESQEGECTSSIFNQKRTLKEATVLKQKVGLQAEFKGEVKEGSASGFFKTCVSLNGGCVVDLYKRDFSYQGKPLEKFEIKGEKSQRIFSIIILIMGYPVTVGADYNYSWGFIESPIKTENEFGISQYIYASLSVTAYGELNLVHIIKIRAGVQGYIFKGSANGVAQFMINQVDNVIIPEKYIVFLDFQIESLTFDVYASYQHITFRLARKCFRRWRFRICWYVPQIGYSNWSDVYRKSFQLANFQAKKRIFEIKSKCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SDR3</accession>
    <name>A0A8S1SDR3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thioredoxin-like fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0050364</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SDR3"/>
    <dbReference type="OrthoDB" id="289091at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02972">
        <property type="entry name" value="DsbA_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33875:SF2">
        <property type="entry name" value="ACR183CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33875">
        <property type="entry name" value="OS09G0542200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13462">
        <property type="entry name" value="Thioredoxin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035784744" description="Thioredoxin-like fold domain-containing protein" evidence="1">
        <location>
            <begin position="17"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin-like fold" evidence="2">
        <location>
            <begin position="37"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8136824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="217" mass="25034" checksum="8A3FFEB1477B6EE9" modified="2022-10-12" version="1">MNKIFLFLGIATLVLSNQYVPIPNKPLGVSKGDSKKLIIEAFFDLQCPDSRNSFRILDQVLQQGYGTQFKYTIHMFPLPYHRAAFPEAQGFAFLSEINTEAAYLFAKTIFDNQETLAEAATLEWTWQEILNKVAQFAKENVYPKYQYDEVKFAKSLLPGTDWNLKARYWWKYGTYRTVSGAPIFIVNGVILNGAEEYDVQEWIDFINQKNPKSAQEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SEQ7</accession>
    <name>A0A8S1SEQ7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0060159</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SEQ7"/>
    <dbReference type="OrthoDB" id="301605at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8137572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="637" mass="75298" checksum="A6D6E5E7EB79035E" modified="2022-10-12" version="1">MIIFRTLLKQRFSTQTNLLNFSFRDNFQHESDDDIDIIDSSKEDVKVVQNEQELMALISQHVDPKILIDIFNKNKYIFSLQHSLLTIRIIAHFLNSVKNYQSEPIFRQGMGEISKLLNSQLEQLTPIDLLELLCFKSKYQVKGQQSLLENINDKMLANNLNQTLQNCSDFSIRHYINIWYDSQVLNMVIPQIHQLVEDRLYTEPLSSMEVILLVRCEMMKSYKKKFVNSTFIEFCISHYDANSLFYDFQQVTQFYIMLLKMKYQYLNPYLESHPILIKMRQFLIDNLNVMDEHTIFSLISNFSLLPQEVNPVLEIKIKEFLFKQLSLRPQKISLNFIQQFLINLEPICTTQEVEKLIDEIIQRLLLETHQHSVQQANKISQIILSLWKLKLTINSRFKLNIFSDYVSQVCKFSFLENGLLYLKFICGQDVDSFLNEENAFSRPLHSYAVIKNEKLKIKLNSYLYKKIKDNPLKIINEILSFQYQDSQIFKDLLPTIITEFMKKTHYQEKQLPSLFKLLNDVKSLNLLKDCLSYKRSDIYRLAIRQHNLTDLQVQIFGGRLLQQIERYHRHGVLNLILNSNLVILIGKKNPEYLAAIMSIIKQQNDGLETYSFCKYLKDNNVDYRKSQIRKNRGSLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SFZ2</accession>
    <name>A0A8S1SFZ2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FGFR1 oncogene partner (FOP) N-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0070292</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SFZ2"/>
    <dbReference type="OrthoDB" id="2160638at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034453">
        <property type="term" value="P:microtubule anchoring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018993">
        <property type="entry name" value="FOP_dimerisation-dom_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15431">
        <property type="entry name" value="FGFR1 ONCOGENE PARTNER/LISH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15431:SF4">
        <property type="entry name" value="PROTEIN TONNEAU 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09398">
        <property type="entry name" value="FOP_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FGFR1 oncogene partner (FOP) N-terminal dimerisation" evidence="4">
        <location>
            <begin position="52"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="129"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="134"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="147"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="161"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8138746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="264" mass="30149" checksum="C51A6D2313193817" modified="2022-10-12" version="1">MSDENAIDLVMKNLDQQGQLTKVKAQIKASVFKILDQSKGKKSGASNFFWENEKAKAIQKDNELLLLLEVVKEFLQFFDFEFTLSVLQSESNHIPFIERDQLIKKIGLKQSDLNVNQPILQHIYQKYNPKGTKQGSQQNTSQQEQVSIKKQEKQEPVKPGSKTNQQASVTKNQPITKTIGKVESTKLSNVKSQSNDFQFEEIEEDLDDVQDLEVPSTIKKNEKQNNLMLTSSEEQLASMSQGLDITVDSNEIDQYDHVVDIKWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJS2</accession>
    <name>A0A8S1SJS2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0070217</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJS2"/>
    <dbReference type="OrthoDB" id="290420at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8138634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="620" mass="73316" checksum="85B84651EBF7F751" modified="2022-10-12" version="1">MRKAYEQDYINNQNAGLLQQDDDDQNDGQTKTINYIRPRPFPHENNRECKLDVVCHENECRILQKLCCQECAHFVHPNHKTDSLDWYINHIREKIKRRHGVQQTTTHEISVLKSEQRKKRMKYLKYIIKQLKKVEKECRSIEDTTEQKIKIKLSSTSRKEAFDKMIRLLKLVRHQNLDQFKQHHQEFLVKDEVDQQMAMQYNEERERKELQRQISEIVDQFFLADERERQKLQHNQSLQTQQQDEIKALRLTGGLWERNMNKLAGALKRFSKRMLSHSKMSIRPNSSTHNIVLVEVDLATNEDDPESTPKILQILPLNSENLITVHPNEIKLWKYSKCYLEFKPIAPLPFLNLKYVCVADKKDEFVVLFGDNSIMSISSKSMNSKNKQFTCPKQGMTLYRILISPCQNFLIASGGCKQNCFYVIIWHFNDAKIRKVLRYQYSFPNEGDLLMSETNQHIFRCNKNKIDVFGLIDGVKKQTLQLQNVRDCIEQATILHNFQEQDTLCVSTNRGFYVYTYDDNVVENNYKLVYSFSQTCHCIFHVFYQYGLEEEGFMLAALENNETKIKFQDLNNKILLSGIQFDDQILTGRLIFQREFQNQQLVLLLGFESGKMRVLKFIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SNZ0</accession>
    <name>A0A8S1SNZ0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0080463</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8140122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SNZ0"/>
    <dbReference type="OrthoDB" id="1926336at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="263"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="362"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8140122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="933" mass="110230" checksum="8412FB486FC76E5A" modified="2022-10-12" version="1">MKSVQKPTQISGMNRQFSVSKDMGNFSMQSPDKQIYCPKTQSQAKIQQLGPSPYMQSPQFDAKQVSQNHVIIQPSPPQKLENSYQYKPSIKNGSITNEIFKPNNQQEVIKSQNEFKPQYETYKYEYNKTFAGTFQSDANKEQLTERKSNTSKKDGTTLIDQKENYQQFQAKSPFTDNHPTVGNSRLSSQNINLIQSKPKIQTEIYRISGALRDLMSSVQKMMGDGRLPDQINDKMIEILANFRKLEEIANNEPINGSDNDSKISTMAEDYNKLKSILEQLQIKMTALVQENQKLNKNLIEQEQKVTEEIKKKQLAEEKANQANKEFNRVFECLQINQKENEELKNKLIQFESYKNLSSQTSNRLQSDEINNYKMKNQQLQNEVEQLEIKYKQLLTEKNNQKEIPKSPSILKSIHSSSTKQSEQLELKLLQLQIENDNLKAEMAHNQVDSKVIDNKNDMIQKLEDKIKSILKEKSISEEQSIQICHNLEIMNEQLKNGVQNKEIEVSNLLKQKNELEREHRNQINYLNQMLKGTEDKVQIAKQDYQKLLDEFKQIDIKKGKLQEEIRNTERKLDQLNQELAFTRNELKKSTDKNHQLQTQHDQMNQQLQQQAQLQQQLLQQIDGLKQNEQQIRVEYEKLEKQMQAVKSEMKYQVTELNEMIQLQARKTQEKERQLNELIEEMGGMRDQYEVQKKVCQENVNDLERRLKNVRLQQEELLQVKEQEILELKQTMEQQIKMLDKRNALSSFDYDQREQQLQVELQSKNDQIENLKSEVEKVRSNNFNIQEELLRQGMLLNQHQATIANYKNELIQSKQEQQHLSEMLKKRKEETEQLHQNLEEMRRELQSKKMSDDSRRSVLRQQDLQIALENLSRENLSLQQKINGLSNEMNRKSRELSERNEEYQILKRKYDETVANLERLEKRWGEKIDLHRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SQI2</accession>
    <name>A0A8S1SQI2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0100468</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8142108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SQI2"/>
    <dbReference type="OrthoDB" id="448960at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="174"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="208"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="3">
        <location>
            <begin position="367"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8142108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="462" mass="53538" checksum="2ABDE1EEDFDACBCC" modified="2022-10-12" version="1">MAANNDRINSIQVENILQSLNYNESQIHSIVSQIESEIDINQFQQLLQPVEQEIPIDKLTSEQIYVIQGEFKQALHLQHIEKVKHILNKYQKEVDLVNRIDPQTRQISTFIAIQGSDEEVSLQLLKLLCELGANVNFRDNLKQSILFYVCRDGKTKLFDYLITQGINISDPDSYGQTPLFYASRENRVDIIQKFIRLGADVAHLDTLSCQTALFYAASKGHFEACKLLIEAGCPVNHLDNKKKTALYFAKQSQKKEVIDLITASMSKQKEDVHQKKEEVVKVTEQQKQIKKKQKDVPKQQYKILHTDDKGQQRELTNEDFSRFQRQYPEIAQLILNADDIIDDNMINQCKEDETWEKIAKKLIAQIWKSKGAYFFHKPVDQKEYHINDYFEIVKRPMDFGTIKNKLNVSAYKSCREFHADMLLVFDNCALYNGTQSAIGQIGVNIRNEYLSLKDQFGLSKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SQP3</accession>
    <name>A0A8S1SQP3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0120421</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the JMJD6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SQP3"/>
    <dbReference type="OrthoDB" id="424465at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033749">
        <property type="term" value="F:histone H4R3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106140">
        <property type="term" value="F:P-TEFb complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050910">
        <property type="entry name" value="JMJD6_ArgDemeth/LysHydrox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12480">
        <property type="entry name" value="ARGININE DEMETHYLASE AND LYSYL-HYDROXYLASE JMJD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12480:SF32">
        <property type="entry name" value="BIFUNCTIONAL ARGININE DEMETHYLASE AND LYSYL-HYDROXYLASE JMJD6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="JmjC" evidence="12">
        <location>
            <begin position="131"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8143821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="353" mass="42391" checksum="7B2E026FEAECEBAC" modified="2022-10-12" version="1">MNQSQKQFEFKNVDELKIFLKYQQRASLQYQQRFKDLLLQKVGQSPIKFDSNLSIKEFQTQYDAKVPVIINGLDEDWENWNWDYLIQNYKDCQFRCAIDDDGNYLKLRFDQYIQYFQDNSDYNPLYIFDGDIPKNMLNQYKIPYLFPQDYLAYLKQKRPQYRWILCGPKQSGSMIHIDPYETSAWNCVVLGKKRWVMFPPSIDKNIVKGKSLISQEVLSNINNPIDYFSIIVPLIKEYCDQQNIQYYDFIQSEHETVYVPNGWWHAVLNIEDCVAVTQNYVSDQNFEKFWIAFNKENPTLSQQFQTNVQQENEELYKKILILNERFNIQIKSLQQDDNVSEQDSLEEFQNQFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SRT0</accession>
    <name>A0A8S1SRT0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0100477</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8142126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SRT0"/>
    <dbReference type="OrthoDB" id="284123at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050313">
        <property type="term" value="F:sulfur dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070813">
        <property type="term" value="P:hydrogen sulfide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07724">
        <property type="entry name" value="POD-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00158">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000030">
        <property type="entry name" value="Metallo-beta-lactamase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000110">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000148">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051682">
        <property type="entry name" value="Mito_Persulfide_Diox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044528">
        <property type="entry name" value="POD-like_MBL-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43084">
        <property type="entry name" value="PERSULFIDE DIOXYGENASE ETHE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43084:SF1">
        <property type="entry name" value="PERSULFIDE DIOXYGENASE ETHE1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="295"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="393"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8142126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="474" mass="53315" checksum="94849674FD16EC0F" modified="2022-10-12" version="1">MIKILQKFNRTLFPFSKEVQLKQSETLGKGFYQQQIFTGCLAEYAYYIESNKEAIIIDPLRDIQPYLNIAKERGAKIKYVLLTHFHADFIAGHLSLQRETDAQIIMGPRATAPFISKVLNDNEYLKLGKVKIQALHTPGHTQESTCFLLYDEEGKQHSIYTGDTLFLGEVGRPDLAVKSDVTQYDLASMLYASLKQKILGLPDDVIVFPGHGAGSACGKNISAGYSCTVGNQKKNNYALQEMDQETFVQLVASDLPKPPQYFFYNAGLNKTNQFQDLNLILKKANTKLTPAQVVSKQGIQIIDSRNSISDGFIPGSINVPLQIPFAHWVGTLLPHDREIVIVCEPGNEEQTIMRLSRIGYDNIVGYLHFDDWKSSGNPINKPTELEVQDFLKRSKLAQIVDVRTKEEWGRGILKHAQLITLSELPQNLEKLDKLRDISIYCGTGQRAKIAHTLLVANGFTRVQYCKVGYDDIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SXA9</accession>
    <name>A0A8S1SXA9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cell division control protein 73 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0120444</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDC73 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SXA9"/>
    <dbReference type="OrthoDB" id="286071at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11990:FF:000002">
        <property type="entry name" value="protein CDC73 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007852">
        <property type="entry name" value="Cdc73/Parafibromin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031336">
        <property type="entry name" value="CDC73_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12466">
        <property type="entry name" value="CDC73 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12466:SF8">
        <property type="entry name" value="PARAFIBROMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05179">
        <property type="entry name" value="CDC73_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Cell division control protein 73 C-terminal" evidence="6">
        <location>
            <begin position="243"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8143867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="403" mass="47522" checksum="9715B40310B9009F" modified="2022-10-12" version="1">MFRRGRNDPLELLRDHVIHHKQIKLRSKNTEHRLLFDNSIEFKCSTETAWKSKSGQEYTLGALWCFLDCHLQGLEQKNYRKKALDLNFEQVFKADNQDIIEYFTGKVDYTDCINADKKPLPQNKKSLQKDDDIEFASQKKVKPDQEKEPLTEKEFNLKVFDEILRFEKPITTRNRLFRVQDRTFDDILKTAQKIFQGQYVGVGGDEEELQQILGTRIQDVNKAKQLSSAKLSYSVLNEFIKSKEKPIIIVPQIAELGNVCLRNVQQFLEQGQYIDPNGLKFTNESRSVQIKVKLRLTDIEQQFIVLDTPNTITNWKRVVAVFLRGSTYELKQFPDQNPNQLFKNIRGYHMKFEEEKIKDLIKQWNVKVLDLHRSKRYQDIDVVNTFWEDLEAFLLRPAKLSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SZ82</accession>
    <name>A0A8S1SZ82_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0130278</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SZ82"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="11"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8144547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="17993" checksum="B75E09F99C8750FC" modified="2022-10-12" version="1">MDKIYRILISIINLQINQKELIQNINQYQRSLISKKKEYLLNLIRWPDDNQVLIEIDKFNLFQYIKFLFKSKFDYQEEKDQFPYRAFQAILLFIVIEILLNLYYYTCKDYYQEYQREDIKPHYNYKTLENKLRYSILLFKRLAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T0F8</accession>
    <name>A0A8S1T0F8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0140044</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T0F8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="591"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="89"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="289"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="591"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="608"/>
            <end position="623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8145039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="813" mass="95178" checksum="23BCA47909201592" modified="2022-10-12" version="1">MSTARFKKTEVRIQEMQTEIDVLMTQLQSNSKNISSSQSEHLQMLIEDIHSTNFQTQELSKESEQLTDALYSQSDHDVTLAQAQMKKIQGSLNQSLSSTKQNLQNVEREIEQIEKGLYTIQEQNKVYTESLCKFKENLKMCDSQVVKVLDQNSYNNWNKIKQYFDIDIIQTSVPLGLIMKLQLYILKLTEVCNSNQIKGKQQINEEVANLLIEINSQHLLFQNINLLEQYITVVLKSNNKVNLFIFIQKCLNSMHFNFCQVAQIFNQHNISYKIQQSISQEISSKNHSIKQLQMKKSQLIIEMEKYKKQNKDLITQLTLLEKQLEKQIEAETQHLLAMYMRDKEQSINEIKKKYGKKYFDGMLQDVKQEFKKICLTQQVQYANSIEQAFLRVDVINIQLMNNKTKAQQLIQQYSQQKFKKASSLIIQLNGIDKREYLLELQQCKISLLQIENKVERKIIVQDLDELILQIQYQINDIKLRIQASNVPQKNRSDSIDTQRKTNRIIEHHRSQSQTPIQSPQENASQIKLQLLNINLPIKNPLDMFDSSDDPITSNNSHIQLSFCENQSNKSKDQEKLSKTILVQYNLSNINTQQSNNTDSLTNSRNDENQNNLNNQQNLHQSNPQKKPLLKEVKNIMNNSEQIQNSLKSSRTKKDIQQKQNFTSHKNKLNILIYLKTQESDLAYDPILNYEHDPFIFGYRLFKIDDQLNVYKSQEERINLQNFDNLDDLLNQFNIKQTQLENIKEIEINNCSMTALKFQNIYESTLRHACVDSLALRHLRVRFFPFIFVGNQQSHVGLAVKKDDILSLQKLFSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T3X0</accession>
    <name>A0A8S1T3X0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RNA helicase</fullName>
            <ecNumber evidence="4">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPENT_87.1.T0180072</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DEAD box helicase family. DDX21/DDX50 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T3X0"/>
    <dbReference type="OrthoDB" id="4255at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12937">
        <property type="entry name" value="GUCT_RH7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001645">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059027">
        <property type="entry name" value="DD_DDX21-DDX50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012562">
        <property type="entry name" value="GUCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF19">
        <property type="entry name" value="NUCLEOLAR RNA HELICASE 2-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26142">
        <property type="entry name" value="DD_DDX21-DDX50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08152">
        <property type="entry name" value="GUCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="56"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="262"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="585"/>
            <end position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8148451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="665" mass="75915" checksum="598F39A24A83AFB6" modified="2022-10-12" version="1">MLTRRKSSELIEPEILKKIKGNEESNLKAYIKNKQTRKVLESRGFTNLFPIQQQCFETIYNGDDIIGQDRTGSGKTLAYCLPILERIRGLGLKQNKNPYVLVLLPTRELAIQVTTEFNSILHKEHEYRIYSIYGGTDLRNQIDQVRQGCEIVVGTPGRIQDLLERKVLKLDEIQVVVLDEADQMLNFGFQENIEKIMSYFNDRKIQMLLFSATIPDWVKELSQKYMESNTKHINLINRNESQTSKTVKHYALQCARNQLSGAIGDVVSVYGGRHARTIIFCETKRECNEIILHSKLPAETQPLHGDIPQQQRTVTFEGFKNGKFKCLVATNVAARGLDFPQVDLIIQCNPPKDIESYIHRSGRTGRAGKDGICITFYSKKDMSLIERVERVAKIKFIKISTPQHQDIIKASSRDLQTSLQVVSKEIVDLFLPVAQEIISRCDPVEALARALACVSGYKDKLQNRSMLGSFEGYLTYVLRTSTPFQACGYIWKFLKNNFSEQICNGIKGMKKLRNENGVAFDISEDVKEEFENQYQEISQNGYCRGIEIEQATTLPDIIEEQFQAYQNNYYQQPTVQVSQAVKKEQEIFIGGLDFKISEDEIKNEFKNRGIELLNLRLLRGQDGQSKGSAFGVCKTKEMVSVALTQNGTKFRGRNIRVNMSNEKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T457</accession>
    <name>A0A8S1T457_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Alpha-1,3/1,6-mannosyltransferase ALG2</fullName>
            <ecNumber evidence="10">2.4.1.132</ecNumber>
            <ecNumber evidence="10">2.4.1.257</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">GDP-Man:Man(1)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0150315</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Mannosylates Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>a beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + GDP-alpha-D-mannose = an alpha-D-Man-(1-&gt;3)-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29515"/>
            <dbReference type="Rhea" id="RHEA-COMP:19511"/>
            <dbReference type="Rhea" id="RHEA-COMP:19513"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58472"/>
            <dbReference type="ChEBI" id="CHEBI:132510"/>
            <dbReference type="EC" id="2.4.1.132"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8 10">
            <dbReference type="Rhea" id="RHEA:29516"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>an alpha-D-Man-(1-&gt;3)-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + GDP-alpha-D-mannose = an alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29519"/>
            <dbReference type="Rhea" id="RHEA-COMP:19513"/>
            <dbReference type="Rhea" id="RHEA-COMP:19515"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:132510"/>
            <dbReference type="ChEBI" id="CHEBI:132511"/>
            <dbReference type="EC" id="2.4.1.257"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9 10">
            <dbReference type="Rhea" id="RHEA:29520"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endoplasmic reticulum membrane</location>
            <topology evidence="10">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the glycosyltransferase group 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.132" evidence="10"/>
    <dbReference type="EC" id="2.4.1.257" evidence="10"/>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T457"/>
    <dbReference type="OrthoDB" id="448893at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004378">
        <property type="term" value="F:GDP-Man:Man(1)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102704">
        <property type="term" value="F:GDP-Man:Man(2)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03805">
        <property type="entry name" value="GT4_ALG2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027054">
        <property type="entry name" value="ALG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45918">
        <property type="entry name" value="ALPHA-1,3/1,6-MANNOSYLTRANSFERASE ALG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45918:SF1">
        <property type="entry name" value="ALPHA-1,3_1,6-MANNOSYLTRANSFERASE ALG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="2 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase subfamily 4-like N-terminal" evidence="12">
        <location>
            <begin position="19"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="11">
        <location>
            <begin position="213"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8146379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="414" mass="48357" checksum="ED110CEE246204BF" modified="2022-10-12" version="1">MQQQQDQLKIAFLHPDLGIGGAEQLVVNLALALQKNHYVKIYTPHHDPSHSFPETNGQIPVEVRGNIIPAHFFGYCTAMCAYIRMILATLYIIFFSGRWDVIIIDQVSVCLPLFWLFRRKTIFYCHFPDKLLCVERKSFIKKIYRFFLDSFEEISMLFANLVLVNSQFTREIVKQAFPLYNKYGRQPEVLYPAIEFSKFEMAPELSRHDSRLTSNNYFLSLNRYERKKNIALAIHAFAAFRQQLHSDENIETIRLVIAGGFEQRVEENAQHFEELNQIAQRQNVAEYVSFKKNISDSERTWLMSNSLAVLYTPEREHFGIVPVEAMYNQVPVIACNSGGPKESIQNGVTGYLCESKTNEWCQRMIEIYQNREKSREMGERGRDRVIQLFGFAQFQMQMDSYVRMVTKTGKQKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T6S7</accession>
    <name>A0A8S1T6S7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0170215</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T6S7"/>
    <dbReference type="OrthoDB" id="306614at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8147933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="179" mass="21188" checksum="B0D558E70DBF2B55" modified="2022-10-12" version="1">MQNKVQFIESNDTEGILQVPDNLIGQVSKILKDQFGFSIEERVGAKSQQKQQVFQQQLNLIVQLVLCSFKKYCNINQFTTIKQEMENIQQKKQFSKKFSLYDLHQLLYENQFCQEFRDVFENYLSNGSAEKDLKESRKITQDQKKELKKIISELLLEVLKNNPNTSLFNHQFNRKKINN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T7J6</accession>
    <name>A0A8S1T7J6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">GPI transamidase subunit PIG-U</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0160180</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGU family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T7J6"/>
    <dbReference type="OrthoDB" id="549017at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009600">
        <property type="entry name" value="PIG-U"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13121">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13121:SF0">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN ANCHOR BIOSYNTHESIS CLASS U PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06728">
        <property type="entry name" value="PIG-U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035788738" description="GPI transamidase subunit PIG-U" evidence="11">
        <location>
            <begin position="24"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="62"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="140"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="273"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="317"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="350"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="385"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8147044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="407" mass="47951" checksum="40E6E7BCDA9C07A6" modified="2022-10-12" version="1">MYRIILTLKCLLWLLGKWDVDLTWLPYFSNPYTQYNNIREAKYLLANNYNPYNTDGIFQKPLIVYGLVLIDNDIIFLIADILVCFLIVQLFGGKQGYRKSILLFYHLNPIIFANLILKNTNVFDHIFLLLTIFSALKIKILSPILFGILLYLNPQYFILLAAISVIFRRETSTTLKFAIKLIVFLFVCIATICLLLYISYIITGDWQFIEKTYIDYYFPKDHRPTIGFLWALFSGLFSKYKCLYHAFFMLLPSCYILPLYNLFVKYAKREKEYLGFMIGVSLFCSFLYFQYIAISDLVIIFILLFQRYDWLFNSLPTIVIIHAGCLILFLGGGLQLTWTHSFTGNPNFTFFQVFVSYVFYVILVSEGVREMLKFRTIQKKEEKENMKLEGEDEKKENQQRNKKVKGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TCZ0</accession>
    <name>A0A8S1TCZ0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0180237</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TCZ0"/>
    <dbReference type="OrthoDB" id="10360997at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8148779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="180" mass="21440" checksum="98CFF0C95EED9D4A" modified="2022-10-12" version="1">MNEKIIRPNTRINNRNRIIDKIFSQKQKNSFNSVNYKSYVQNMLQQIKLKEEVRNQSSNLYDSKYTWISKISPQPRRKQCVPQNLRLETLRNTQRVIIKKKSYDEDKNYKQQFSLNSNTRFTTIPNRLDSKQTIIVSPILIKDTVPVKEQLILGDQSRLFDFNNFSKFSSWSRKSSKSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TDS7</accession>
    <name>A0A8S1TDS7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Poly(A) RNA polymerase mitochondrial-like central palm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0190265</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TDS7"/>
    <dbReference type="OrthoDB" id="273917at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031499">
        <property type="term" value="C:TRAMP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043634">
        <property type="term" value="P:polyadenylation-dependent ncRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045862">
        <property type="entry name" value="Trf4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092:SF48">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092">
        <property type="entry name" value="POLY(A) RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="2">
        <location>
            <begin position="279"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8149744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="618" mass="71826" checksum="FD9623F7481FCDA7" modified="2022-10-12" version="1">MNQDQINSFELVDKQDTTQDEIHFNECYIDQSVNPCQEIGINVQVSGKSYEIQNDTLEWNLVEKDNQVLEDKKIIDINNNTQNDLDDTWVVITKTKKYKKKSNRKLTNSQDDQSKQQKSPQKPIPPTITEQSVKSNAHDIALFQNQKNIPQGIETIKQKVFIANTEKILKSENQEIDNRSQQNFLKEDQTSLTQLEQVNNTKQSQQFIQIMDLNLKIPQECNKLFKRNTNVNHHLDCQNSPFKQTIKEDQIDQHIMNQARALMIKKLDMDMREFNDIILGQNQQILKLRRIIYDRLQFVINYLFRDFNSQVLLFGSCATGLALPESDIDIGITGFEMCSTQQLYIPIQKLTEFLQKMRWVNNIVVITSSAMPLIKLQVDPIISFVQSSLPIGLPYIDLIINPDENITRQIFSVDISFIQYSGPKQNQHLGLISTELTLQWLSFYSELRAIVLLFKSLLKKRGLNDQFKGGMSSFCIIQMVLAFLECFYHQNQASSIGYTTYNFLKFYGTEFDPKTTGISYKGFNENPFYDLNEFDDQSEITIVSPITNEIISSATSFVLTILQDLKALYQATENEVIFFYEKLKYNKKKKGKKEERNLFQKELNKLRPLFSTTVYNKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TL42</accession>
    <name>A0A8S1TL42_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0240331</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="298777at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8153645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="432" mass="48788" checksum="BE12A85C82478123" modified="2022-10-12" version="1">MIIFTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIGKWVELSEGFWDNCQVILKGEYKNGNKIDLWTIEAFGQSIGGGSYNQDSMKIGNWIELCDGFNGAAQIIYQGQYLNGKKVGKWDICYRENENQTFMKIGGGLYDQIASVKIGDWIELSDGFWDNSQVTFNGGYKKGKKVGRWSSYLKKNYGDFQNVLIGGGSYNEQDSLKVGEWIDVKDQFIVSQQLVYKGEYREGKKIGTWMEIEIRKNQTRGIVNYDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TQN6</accession>
    <name>A0A8S1TQN6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0250033</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8153792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TQN6"/>
    <dbReference type="OrthoDB" id="310866at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01053">
        <property type="entry name" value="LSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="278"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8153792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="300" mass="35477" checksum="88DFD406625A82FC" modified="2022-10-12" version="1">MNQNDANLGLLSNKSNARYKEPKQNNDDFEIQDQGDDQEYVSLDDAKVQHAEVIQPKQIEQPIVPPQNNIQSQSEFQKNQNQQQAYQQQQFPKQSQIQYQQTQINQQQNQYSQYQYNPYQNQQYSQPLNQIYNPVSNVFQQTAILIDKSINQRTQAYSTCIHCKTPQMIQDEKQPFMCYRCQQINKPNFGYFQCGSCKITVMYQCGLSNLIKCTKCQTMNYVQQPNLPNTLQQYQQSQQQQNLPQQQQQQQINSSNSQLFQSQNQYTNQPKLYEYSQYQQSNKEQKSIGRNQVDPMYQEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TUX8</accession>
    <name>A0A8S1TUX8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CTLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0280049</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8155938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TUX8"/>
    <dbReference type="OrthoDB" id="972532at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051350">
        <property type="entry name" value="WD_repeat-ST_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22838">
        <property type="entry name" value="WD REPEAT PROTEIN 26-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22838:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CTLH" evidence="5">
        <location>
            <begin position="56"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="265"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="307"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="475"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="520"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="520"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="533"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="548"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8155938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="557" mass="64694" checksum="DED4CF489AB9B3F9" modified="2022-10-12" version="1">MQVFDNLALQEVQSISIDGSPDRLKEIIRMILQELNQMGYSDVCNILEEESQTKLDTTQMNELKESFLNGDYEKLLQQLEFSETINRKLQYEIQKQLYLELVQQKNYEQAVNLLRQLSEYNEKESQVLAKMIFLQEDASQLYIELAIPIDQGEQRLQLYQKTLNLVNFGDFSLKTQFDKVIDQALEYQIKQCPYHNDSNENKNYSLLLDHNCQCYQIPKNNSIIFQDFDDEVWIGKFSNSGIWIGCATRKNSIYIISKESQQKISKAHQMDINSLVFTSDDKLLFSASNDKKINGYDVQTAQLKVTINQHTNEVLCLAYFKNQLFSGGVDGWIGIWQDNGKLINQVKSKVIKNILITDQYMLLHNAAVFSITVIENDFKFNKIITNLEEQELIVSSAISQNQQFLVLNISKEKPKLHLWSLKTFQKLHVFFGFQVKGIEMKCCFGSKNDNYVCVGSQDGKVHFFNKNNQLQQNIIDGHDQAINYVDWHSQTAQLLTASDDKSVKIWIQDQVTQQQIINVSNEYDQPSNSQYGEIEEEQDDHEDEEEYQQQSEGDQSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TXJ7</accession>
    <name>A0A8S1TXJ7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4200 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0290292</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8157008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TXJ7"/>
    <dbReference type="OrthoDB" id="10264298at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051147">
        <property type="entry name" value="CFAP_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025252">
        <property type="entry name" value="DUF4200"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21683:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 42 LIKE-2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21683">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 42 LIKE-2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13863">
        <property type="entry name" value="DUF4200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4200" evidence="3">
        <location>
            <begin position="21"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="88"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="186"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8157008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="327" mass="39599" checksum="734B295D0B623278" modified="2022-10-12" version="1">MDRKVRKRASSVNTTNPAMKLLEKRRLMFEVHEAYENQLFQFKQQEEDFKTKENEIKQKDEEIQTELLKFCKELQVNETKKKKAISLYQSERAEKEKKIKEIAKLQHDLSQDQQIFENVERKAERLKKYVEYLNSIIKAYPDKYSDLDSITDRYKRLVISHQILKQEHEKMETDCEKMKFIITQYEKEKNHEVLQLNNDIKEYQKLIEEKMIERNQLLSEKEEEENRITEENLNLGRIFMAIDNINQRCLDGYQKIKQDYEDQNKEKDKAMKQIREDKKLLKQSQDEENFDVKCQQASIKLKQIVQNLNDFKKIIEGCKNEIKNKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TZT7</accession>
    <name>A0A8S1TZT7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0330006</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TZT7"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8159201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="57" mass="6511" checksum="97E58366F45EE392" modified="2022-10-12" version="1">MDIEIPGRNGYQTRKQILELVSNKQMTVICPAQDTQKNLIQGQKLGITSFLQKHVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U539</accession>
    <name>A0A8S1U539_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0350063</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U539"/>
    <dbReference type="OrthoDB" id="298600at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045192">
        <property type="entry name" value="AP180-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951">
        <property type="entry name" value="CLATHRIN ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951:SF108">
        <property type="entry name" value="ENTH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8160591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="430" mass="51432" checksum="82788FFA01FF4B10" modified="2022-10-12" version="1">MNFITRFCATVGAETAFERARLAATYENTYPPDESDIKIILNALSGGDKETDRRIAFGSIVRRLKQNHMNEDGWIIIIKNLIIIDRCVNDGILLKEFCELDLPNIENRSEINRNNNKLLINELISKYYNYIKFKSQQLRTSFSLTLYKKEKYLHFNQLSVGQTQNEINLIMQFVDKAYEVIETKKNGHLKYKLNQYVFLLILNDLFKYYSCSLVAFNILIKKIEQLELEDLLQMAEITRLLYKFCNQLDTFINQNRFIQGFEYYDVEYKITSDLVELVVQYMNLYEQTQKGTPFQPKEIKFNTQKSKLVLEKIFSQSLNKTQSDFFENYQSNIKQQSKLVIYCNQFKHKFDESTFQEFQNNFSSNYIIIQNHYKQNKDIPEFKEEDEDLDFLTKIEENNTNYKFDKQIHLLYPEAFNEKMFLNFENIYYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U6D2</accession>
    <name>A0A8S1U6D2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0340209</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8160184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U6D2"/>
    <dbReference type="OrthoDB" id="310807at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039273">
        <property type="entry name" value="TEPSIN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21514">
        <property type="entry name" value="AP-4 COMPLEX ACCESSORY SUBUNIT TEPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21514:SF0">
        <property type="entry name" value="AP-4 COMPLEX ACCESSORY SUBUNIT TEPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="325"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8160184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="516" mass="60485" checksum="7A4638ED816A1F2B" modified="2022-10-12" version="1">MSSILNKFRKQPQNDVPPGIKIENTLFGQQQGTYQPPVIPSVQLPQTTQPRQKGRPGGGWDDEPEPQQQQQQTLSNKPTNTINLINDTPPKQNENKANIDILLPPQSLNNIQLSRVTDYKKGQLLTYEELEIGGFEFEFHIVEEVIKLGGIKLKQSDSVLKEFARACQTLQEQLIGSILLNKLYGEENWKVQIRCIYAIQFISQQYQNYREFFQINQNYLKIDTDQQILQSAIDQTLLEINGQQIQKQKEIQLEFREPPKPQQNIVQQQTNLFEIGNNEGQQKQQQQQKLDLKSLKIKQPEVKQQTQKQQNTQIDLLEAFNQITLSNYDQNQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQGISFDEILNTQPLAQTVQQPQQKKNAFGFLKKDQQQQPQQQQPQQQKQEQVQLNSLLLQTPQQLYQQPALNQQQTSNIIYGQPSNLYNLQQQAQFGQPNYSQAFAQFNIQQQQYNLVQQTQFTQEQPQQIQQPTHKENAFDFLNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U6I9</accession>
    <name>A0A8S1U6I9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BRO1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0340241</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8160248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U6I9"/>
    <dbReference type="OrthoDB" id="2141925at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09034">
        <property type="entry name" value="BRO1_Alix_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025304">
        <property type="entry name" value="ALIX_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004328">
        <property type="entry name" value="BRO1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030">
        <property type="entry name" value="PCD6 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030:SF30">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13949">
        <property type="entry name" value="ALIX_LYPXL_bnd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01041">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51180">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRO1" evidence="6">
        <location>
            <begin position="1"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="752"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="752"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="787"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="842"/>
            <end position="869"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8160248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="869" mass="100767" checksum="97FD8D252303B430" modified="2022-10-12" version="1">MLFNLPLKQTRHVDIIKPVEQAIMNCYGEVTLFTEIRDVVEKTQKLRQNLDYEKINLSIATDPLTSENLENNLLEYLKNFSLLFKHLRFEQGDQRSPQVMFYWSDSYDHKRSCQLNSGRLELISQYYNLAISYYYQANSKIIKDTDPDRKAAYTKLRNGLWALDQLKQHIHGLSKDSISQLYDISTLNLEFLEHAFCGLAYKALYQNMMSQVKTFGLNNVCGTIFEAAKEFTIALKAIEGMNSQYQKQIGKNFLAQILPMIQFNQIWATVTGCLQMGNFHYGRIPDDSRGQNMGKALGFLIRAQQLIQPIVNDKNANKTYSPDQLDQLKQLNQQLTQLTTEYNYKNQQIYKEQIVAQDLLPYPPLMEQIRLKPMEPVSFKEPAQGANKFSGFVSEEALQLSREIKIFVEQTKYSLEESVRQLFDQKNQAYAETYVTYFIDMAQQKQLIQGGIPQSIQDKISYIRSRGCLSGIEKIVEQAKQKSVTCNQLLGQIEQALLNEQQQDLDNRNKYGAQWKRIPSNQLNQQYFNSLKDLKAKYLQAQKIDFDVMSGFENSKEALILATQNDNIIIQKLPKDNNNSDFVNQNAQTFVQLTSTDQQVNSMVDQLKAIIQEYNQIMQETNFTKIAVNGINEGIPKEQVFNMAIQNVQTLIENFNNNMNKVSEVISQVCGLARELSNLKKQSAEQVQQSGQTNIFAGAQNVIDNIDHSVIFYEQLKMHLEEQDRKVKDFLMARSIESQQLIQQLNQQQTYQQSQQNQVQNPPQQPQQPQQQSGYQYPVYPQYPQYPQQPPNPQYPQQPPNPQYPQQPPNPQYPQQIPNPNYPQQPNPQGYPQQMPYPPQQYPQQQQQAYVQYPQQQQNYGPWGQQQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U7U8</accession>
    <name>A0A8S1U7U8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0350223</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U7U8"/>
    <dbReference type="OrthoDB" id="299126at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000555">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="41"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="221"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="105"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8160890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="504" mass="59473" checksum="0F0B3DA87E936D5E" modified="2022-10-12" version="1">MSKNLQLNEQASKIYMQDILREFGYFKALEQQAEYFDYIEETISSLEYKQVKKGNIVFHCGERGDYFYMILKGTVLVYVPKKDEEIQKQKQIMLQMNQCKEDMANTKKKDDAKQLQKQMQDLQNQLKEYQGMEDILLFPFKSRYYQKLPNGQMICLYKKVNTMKDGDCFGEVSLFRNEPRAATLIALDTLHLGALNKSNYLRIFESKLEKLNFTLGMLSKMFPQSSKEVVIQLSFDFQRKLFSINQTIFKQGDIVDGLYLIFQGIVEISSDNVRVNQFSECQFVGLFDLKNPGLRQYTATSVSYETIIYFLPKKQCSGLDRFMRERINDYRISSDGFRQDWVKKCHKMIAKQQQDTNKVILKDINTKEILQKCITQRESKTTSNTISKDRINQIVEYNNHQCSLKEKLKNVKLCLQMRDFDKEKELVEKMMKQQYTQLPRLRERPRNLFETYTNLMSKVSRLSLSPNFQSPNTSDHSIKNIHVEKKLQQRLKKSEQIINQIMGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U8E6</accession>
    <name>A0A8S1U8E6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S ribosomal protein L35</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0360208</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL29 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U8E6"/>
    <dbReference type="OrthoDB" id="295891at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3450:FF:000001">
        <property type="entry name" value="60S ribosomal protein L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.310:FF:000008">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00374">
        <property type="entry name" value="Ribosomal_uL29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001854">
        <property type="entry name" value="Ribosomal_uL29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045059">
        <property type="entry name" value="Ribosomal_uL29_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00012">
        <property type="entry name" value="L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45722:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL29-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00831">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8161481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="125" mass="14633" checksum="BBDB5FDE6FB8B6E2" modified="2022-10-12" version="1">MSSQKFKVKKLRDQKPEDLIKDLEKLKGELIQLRTVKVSAGNAQKLGRIGLVRKRIAKYLTVINEQRRNSVKNAAKSITKLPVDLRGKKTRAIRQRLTRSEKAQKTQRQWKRLNNFPLRKFALKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UC37</accession>
    <name>A0A8S1UC37_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0360234</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UC37"/>
    <dbReference type="OrthoDB" id="285405at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06606">
        <property type="entry name" value="STKc_MAPKKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF56">
        <property type="entry name" value="MAPKK KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="63"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8161533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="382" mass="44049" checksum="63020607C5730CD7" modified="2022-10-12" version="1">MGLYISRFCKYFRGKESRVYQQQNSGSISQHRSKILVQNNSDGYDSEPEQHFHHTESLVKIQWKKGELIGQGSFGRVYKCMDIKTGRILAVKQIELGYVDKESLESFHQEIKILQQLKHKNIVEYYGCDEDKHHLSILLEYVGGGSITHMMKKFKFNLIEPVIQKYVTDILHGLVYLHNKGIIHRDIKGANIIVDTKGVCKLADFGCSIIGLNAYSLKGTPNWMAPEVINSQETGRYSDIWSLGCTIIEMLTGKPPWGRFQSPMQALLTISSKQCSPPIPINISSNLKDFLDKCLQFDHKKRWKAKQLLQHPFIIFMPKKISKTDQFTPLQKPTEEHNNFDLHVAPQLEDIKKDEEYLDRESYPMILSKRSSGVSDEKHLQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UCU2</accession>
    <name>A0A8S1UCU2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CBM20 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0380213</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UCU2"/>
    <dbReference type="OrthoDB" id="550577at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001070">
        <property type="term" value="F:starch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05467">
        <property type="entry name" value="CBM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002044">
        <property type="entry name" value="CBM20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15048">
        <property type="entry name" value="STARCH-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15048:SF0">
        <property type="entry name" value="STARCH-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00686">
        <property type="entry name" value="CBM_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01065">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51166">
        <property type="entry name" value="CBM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM20" evidence="1">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8162735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="182" mass="21031" checksum="A50D5704CC761706" modified="2022-10-12" version="1">MSAQVLFRVKCSTEFSQMVRIVGNSAQLGNWNPQQGFKLITNNEMYPIWYSDFALEVELNQLVEFKLIITDGFNSFWECGENRYLQIQGQKMVVVLTYNMPSIFIYNLKRIFSDNDLTTIAQGRTRKVSLQLQDKLYDSDDDSDSDQESDVNSIFQDEIISCNSSENITNSSNLEPTFYGEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UGM1</accession>
    <name>A0A8S1UGM1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0400241</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8164138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="299083at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="90"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="113"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="228"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="809"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1010"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1110"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1318"/>
            <end position="1338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="732"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1047"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="760"/>
            <end position="773"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8164138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1377" mass="161529" checksum="98EF2E54C41F01CC" modified="2022-10-12" version="1">MSKILAKYMEYDDSALTLPENLVNLLLIHASLLNISYIYYQNSSSWLYTLVIFARPSVSIQISYFYIFGICYLLLQVLCIIKTKLPGKNIVSLINCLMKVGLLNFVIEQFSDIIGYVIAGLILVIQMFEILFIQGTLDINTKNFQRTQITFFTIITLMINIMLMIFYKLNFQLKHIQIIAIVNSAIQALDLIILKNQRSSLIRIIMISIQLVTIYVGIMQFIHFENSFTLVLVPFFFKIISLIKLSDLIYTKDPIINASILMQEKKFYESFLILNKLKDQNFIRQIKRNQLLKKCIYQINQDLQQKVKTQQLLGVASRLIQNDIKNIKISNSLIKIIQWKIKLLQNWKTNEFSEIYKFVNRMLKIKQQIDDYYQTEPNGMIQALVCFFYAEILNDLLEANEMIIHAKKSADVYLQDYTTNKNMFYLTTKYENGQLQMNKISSNAPSFICNKKLEDLIPLGVREWHNKLVDQFIITGKSKFVRQLSQNYVTHGQSIETIDFAIDLAYSDQVNFICLISPTLVKNATLIVDEKYNIACYTNQIKDIKYYKALFSKGSSIFNIFPEIQGITQSCFLENIHINTQKLIFHNDELDDIHYYCNLEIIVKQYQDVMIYMIVKLENIQIIYSNNGNSTKKDTQTNQSSSANVLSIAEERLALIKQISKEDTHTQHQSIEVRGLNDVKSLKEVKSQNKRKSANEIKSLNEEAIKSALNIDIVQPQVFTSRGDPEQHLLVDKMKTESKKQSVKSITQGGEGKPQQVEQQDPKKEELFDREDQSQVSSIRMLRNSKFYRKYDLYNKFNKHMPLKRQHQLLVILFVLCLVFQGLFIIIQLTSLNLVAFAVDINLLEIKNLFFQPLDMFLVTRWNLWTYNFQRTNGIISQEEYNSVSKFATSNLGLGFDSLNSNLKLVLNRQELQGLLQTKYIEAYSYLDTYKSEQYNMTLRTAISVLLNFQYILKMNYIYEKTVKADSPQIFYSFKNYPIMRDIMTQLNSDIMIQTISRGQNFENEMETLFLFQQIFLILMVMLIIYAKIYINKKLILFLHLSQYSDNDAIQQEIQKFKQLLDKLQSDNSYKFNYSLKIAEKEYQFFEQKVEKETRNTKNLRKKRIPPYRFILVLIVFYSIITLNSIINYLEFQKYLKKYPETAYYKKQLADLGGDIPLMFAQREVLYGRKNYMYLDAAYFDRMWYYIMESLNNTKLFTSQDPNFDKMLVTSSFNDFYAEIQLSDLCQYLPGDIKVRGAQLCPTIMNQNMRHGLKAMLIYIQNLIETDVAINNFTYRAVPTQNELEGAFMISEVINVMNSYFYNDLIDVTTKLVDQQQVFNICYLVILFIVLLIIVTEVKNKIYENSKIIIHFVYIIPSQTLFTDDTFERTLRTLINI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UKP6</accession>
    <name>A0A8S1UKP6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CHCH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0410189</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UKP6"/>
    <dbReference type="OrthoDB" id="310526at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055304">
        <property type="entry name" value="CHCHD2/10-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13523:SF2">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13523">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 2/NUR77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8164743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="132" mass="14094" checksum="7CA086800F9F5B28" modified="2022-10-12" version="1">MPRRSGSASRSTASPSRSTATAARPAPQAQAPPQAPARSGGMFSGLGSTLMQGMAFGAGSEVAHQAIRSVMGGSGHSQPVEQQAQQQAPQQQQQTCQSESQMFSNCLQTNQDITRCQPYMDIFKECQKKYNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UKY3</accession>
    <name>A0A8S1UKY3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Beta'-coat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPENT_87.1.T0430152</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="11">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8165838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UKY3"/>
    <dbReference type="OrthoDB" id="10261470at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22948">
        <property type="entry name" value="Coatomer_WDAD_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000016">
        <property type="entry name" value="Coatomer alpha subunit, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.470:FF:000002">
        <property type="entry name" value="Coatomer subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047312">
        <property type="entry name" value="Coatomer_alpha_WD-assoc_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010714">
        <property type="entry name" value="Coatomer_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050844">
        <property type="entry name" value="Coatomer_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056176">
        <property type="entry name" value="TPR_COPA_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876">
        <property type="entry name" value="COATOMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876:SF1">
        <property type="entry name" value="COATOMER SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06957">
        <property type="entry name" value="COPI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23953">
        <property type="entry name" value="TPR_COPA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="5 13" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="47"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="89"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="131"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="191"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="235"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B TPR" evidence="15">
        <location>
            <begin position="632"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer alpha subunit C-terminal" evidence="14">
        <location>
            <begin position="872"/>
            <end position="1189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF06957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8165838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1190" mass="138713" checksum="8804D9B975A8EB68" modified="2022-10-12" version="1">MIVKFHKKTERIKGLSFHPKQPWLLVGLHSGEIQMIDYRFGRTINEFYEHEGPVRSVQFHQSLCLFVSGSDDFTVRVWNYKTKKCQFVLRGHLDFVRCVNFHSELPWCVSGSDDQTSRIWNYQSRQMIATVTGHSHYVMHCEFHPSKDFMITCSLDQTIRLWSIAQLKKKFTSKSIQLGEQTNELELVQILEGHSQGVNWCSFNPKDNTILSASDDKKVKVWKYYDTRGYEVDQYNGHTNNVSCVMFHPFGEYFISNSEDKTLRLWDMKRKIEVDCFTNYELDRFWICAAHQSNNYFAGGSDSALYIFTLFKNRPAVDLVNNNIVYFGNRKLIKILDLQNGSDKTIKNLQELSCISDNLLQDNVEQVLYNIYENVKPQIIVKLKHHSHSKSKGLSKFLVFEQLTNVSQFFLAKSAIFIGKSKILRSKENSEIEIYNFETDNHIPLGHKTDRLFTFIGGKAIYYSDQMINVLDPISNKILNQFPCTNEFQNIKQIKMNDYCILIQTKNGIYIFTREFHPITHIQEKINIKSVLFFNDSINIILYSTKVHVKYLLLNGDTGIICSMETVPYLVSFQNIPEKPGFQYKLYYMDNMDKLLNITIECQEIFFKYALIEKNLQFVQNFIKNHQKLGDLIIAYLYQKGYSILAHQLVNDKRAKFQLALSSNNLEIAYRTCEDLKNPKCYQMLLEEAMRQGNHNIYEVCQQKLRASGELAFLYTITGQVEKLNIISNIAQEQNNQDLRFQTLLNMDNLKQRISFIKSCNLDKLANLSKMAHGLEFETNKNCSEDIEWLQSLKPQTLITPLPIIRSSQHTLFSINWPHNFVDEDELFKLSGDNFKIGNQSKIDIINTITTNHQDLFDKNIQTKQKINIDKTEENDEQFQDCQWDLDEKELLENIEQNQAILDQKSLLYGYPDYKKALQPVEYLITEQYQQCHQVLQQNYNFVSLKVCQDYLKQLALRSILEVPQIPFLMPSPLIVSSSQDKIYINKSFSELLKIGYKQTSDGKFQDSLNTFKQILKQAIFQNLNNDVVPICLNYIMAMKCELAKKDKNISRQIELSCYMAMCDLQPAHRSLTLRAAMSLSYKNKNYLTGIQVAQQLIKLLEQAPKGATYAKPEVLENVRKIFNNCQQNPKNEHQINFEPFYLNEGTKNLCADTITYNNDVKQIYFCSFDKSRHSTKGILCQICELCNIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UKZ3</accession>
    <name>A0A8S1UKZ3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0390139</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UKZ3"/>
    <dbReference type="OrthoDB" id="307805at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8163276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="137" mass="16391" checksum="01162837B7125DDA" modified="2022-10-12" version="1">MYVQNNPHKIHQNRSLSFTENHSKFNNNQLNQFIKEQLFELKRKRKLPSLIQQESDYLMDEINKINNIYKIPNIKTQLAIPKISQLQNSKQNSVNKLKPSIILSENRPYRLRTYLKQTQPIIKINNIIIKNEDPCQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UL80</accession>
    <name>A0A8S1UL80_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0430067</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8165670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UL80"/>
    <dbReference type="OrthoDB" id="305770at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34451">
        <property type="entry name" value="PHD FINGER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34451:SF7">
        <property type="entry name" value="PHD FINGER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="171"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="363"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="363"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="383"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8165670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="393" mass="46611" checksum="8644ED9ECDB050F0" modified="2022-10-12" version="1">MNSGLNNQNSLLQQLTPAQLEEYQQQLRQQQFFTNLLSSNMKTLQFPGIFQQFQDNLIRTSQPQDLFNFNRGLGGAMNQIDYMKLLQKSLQMNTSAINAFNQNQFFCQLGMMGQKKTNTLQNPITVEDDDPPQIQKCQNSKCNNKGDRKTKSKKGETLQFCEKCLRMYNRGNFCDFCEQVYSNGSYDHDEQEWIQCDECEKWNHLNCEAKCRNSNIKEETENKVYYCLNCSKIKKQQQQQQQIQQQKKQEKTTEEYQPKQRTIMECEDARTREKNINFVATKDNKVQFTFRLNLYDDEIKSDLDFLRNSGKKNIKKQNQQDSPIIKQIIIPQQQLLQQQSQQQQQQQQQQQLIQEEKVIIQSSHYQNSDQQMNSRRRTRNNKNRVNYRNLGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1URI8</accession>
    <name>A0A8S1URI8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Structural maintenance of chromosomes protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T0460062</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SMC family. SMC4 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8166777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5575062at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000481">
        <property type="entry name" value="Structural maintenance of chromosomes 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937:SF172">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="13">
        <location>
            <begin position="531"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="290"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="445"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="686"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="1095"/>
            <end position="1125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR005719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8166777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1295" mass="150610" checksum="5A2040227328AD12" modified="2022-10-12" version="1">MQTESEEPSQLIINRIILDNFKSYYGHLEIGPFHHQFTSIVGPNGSGKSNLIESLLFVFGKKASWMRLQKIHQLIHNSADHRDVKKASVEVQFIEQSGNNRNYFSVKRTVHHTGQSNYDINNKHATLEEVTTLLKSKGIDLTNNRFLILQGEVEQISLMKPKSGDPEKPGLLEYLEDIIGSNQYQEQIDKMTEEYLQLDVQRREKGEMMRVVEMDLEKLEPGKDKAVELVRNEIKNSQLQNVQQQIANYKVQTQITKCKENLTQIEENQKITAQESKDKVKDHADTMKLMKIASDKFQKAKQKRQQIKVQLEELQTKDTQNRDEVNNLTSQQQRFQKKLDELIQERNKCIDEVEDLKKEIPAQEKSIKTLKQEKEELEEIVQKMNQQHQQEVRKLVEKKSNLQNIISEPQLQRNQYAKDLDITKNKLNQLKLPETDGGKGIQNSISECNNNINQLQEIVKTLNQTIDEIKNREKLQIEKQNQLKKELKLNEDQLQEVQQQIDGFSIQQNQYNEQNATIRAIMAAAQQGQLKGVIGRIGDLAYIDPKYDIAISTACGKGFDSILVENQQSAEACVNFLKSNRIGRYTFVSLDVVNKMITQDMMQKRGQNPSQTERLFDLIQVKKSEYQAVIFKIVGQTLVCDNIDLARKLKFEQRNPNRFVTLDGKLIEANGVMSGGGQQRRGALSSNNAKQDLNANKNNQNQLEAMLKQFQQNRQQIEQQLIKVDHELQTLIKDGKIIQEKLISATQDLKNQQDLKQDLQQKLEKVKNLEQEKAEVEKQRKEYEKQIINHQANIDTIDKKISKEKKQLEQIEQEISESENKELKQNKEKLQQLTNKFDKDDAEFKKMQTRLVFLDKKQKTLDKDEIKIKDDLEKIQSKIEQVNQEFKKAEVQMIENLALLKIAKEAEENADKEYKETQAKDKEFDELISRLKEKTNKLKQEKEECMSLLKKAQDELSEGLTKLEQIRLNYKEMQNDYEFLNELEEIANWKLQDQQHQQQYPLTTANQNQSMLNISFIDQGDESFDDGRKRGATLAIDLKAILEISVTQELTEEQTIQFCPIEKEIGRVHAHIQAQIREITKDANIKDIQEFKIKYLEYKAKKSDFDQTKQQLQQQKQKIDQLKKERYDLFMHGFNVIGSKLRETYQTLTNGGDAELELVDTMDPFSEGISFSVRPKNKSWKQMSKLSGGEKTLSSLSLIFALHYYKPTPLYFFDEVDAALDYKNVSIVANFIKERTKNAQFIVISLRNNMFELANKLIGIYKTFDTTKTVQIQPELVQMKINSSIENNENINQNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1URZ6</accession>
    <name>A0A8S1URZ6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="5">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0460151</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="5">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="5">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8166947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1URZ6"/>
    <dbReference type="OrthoDB" id="289558at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="47"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="304"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8166947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="485" mass="57112" checksum="1F5C58388EF48A07" modified="2022-10-12" version="1">MDQNPEQDRDWDDLKGVLADDIINKFKEVGPKVYAHQYEFMNYCLGQDINLQSPVIALRSQTGSGKTLCFQSLIYQEFKDKNQKISEQKQIKQKDNTMKEVRMLYPYTIVLGQTIYLKQHEEYFRKHLPERLKNQNLKFQTFFAFTKDNQFQQVKENRIEDADCQVYLCHPSKMLVGLKNRIINVSNLRYLIIDEADQLLEKNEQNQARIETMQLIQFILGQTNNQSYQIIFVSASLKPEEIQSFIKECVQNNIDANKKVDTRFLHKDEDDIQEVICNLRVIPSIQQQISPYTIVHMYKEAVEQPEQILVDLLKDVFDKFTEAQVMIFFNKKTIVNELINLFKKSQKLEYIVKNNWIGECTGDTNQVDRETVRKEFKDGKKKILLCTDVLQRGMDFRKVRVIIHYGLPITPAGEFKQESYYQRSGRTGRAKDEGVVVSLLLDEDIKQKRQEKLVETLVMNNSKIQKYDQKQFCTDLAKLLNFNKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UTL3</accession>
    <name>A0A8S1UTL3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0430136</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8165806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UTL3"/>
    <dbReference type="OrthoDB" id="5590282at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000001">
        <property type="entry name" value="G2/mitotic-specific cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048258">
        <property type="entry name" value="Cyclins_cyclin-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00292">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 4" id="KW-0195">Cyclin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="5">
        <location>
            <begin position="192"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8165806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="388" mass="45568" checksum="DB57D35D351936D4" modified="2022-10-12" version="1">MKSVNILKMLEANKENRNFQLVQNRQNRVQSTLYIKDELSQQNIKPRVIRVSKWNKSQNIRDNSKREDQRHISYELPRQKTDQYQHIQRTLKNRSLTNIQISIINQAQNNNNNNNASISLPNSNKYLVTKGLDKFQSTENYGNPQLVKTYKQDIFNYLEEFNLKLQQQNTISYNAFYQQSQITLKMRNVLINWLFEVHHKLKLQLETLLLTVWLIDKFIETHLVQKNRFQLFGIACLFIASKYQEVYGVPKSGQLSKLCDEVYSKEDILKAEGQILQNINFNLSFISPLRIFEHIAEVDDQISYGFANGDPFLIVATILNIVGKISNKKIDSSMLKVDSNQTNKLTKELIQTWFLDEVNQPFPSLIKKYQHSHYNHVSTINFSLDLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UUI7</accession>
    <name>A0A8S1UUI7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0480060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000048">
        <property type="protein sequence ID" value="CAD8167873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UUI7"/>
    <dbReference type="OrthoDB" id="311115at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008833">
        <property type="entry name" value="Surf2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34348">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34348:SF1">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05477">
        <property type="entry name" value="SURF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="191"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8167873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="230" mass="27312" checksum="A85D450841BBB36B" modified="2022-10-12" version="1">MNKNKESGKQAQNNKQNDSNLNKNKELQKLVQNNKHFEFLSSGKIKCILTHHEILPTLQEFNNYLNGRSYKNALENDIDFTQFEPYIVQHKTDKNKLYCNLTRQNISKKKSVVLKHVNGKRYKYYLSKYLEEQAKEEQENQQENQEDEILNELEELNNLLNDEVEEQQIDKQKNGILKGGKKINKKKVKPQQEQQIEIEQTIQTKQKKQQQQDGKQIKKLKNKQQKAQVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UW37</accession>
    <name>A0A8S1UW37_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0490064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="302167at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="274"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="966"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1161"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1263"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1477"/>
            <end position="1495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8168393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1541" mass="182293" checksum="58B9E0C291D33001" modified="2022-10-12" version="1">MLKLFEPYYRELIFLLSQFLKFEFTQPQIIQIILIVLNQVQPISFNFYHIYNSEKQFEPTLQSLLILTRPYLFIVNEQSKTIVFSICFAIQQIIILTTIYIIIQYKNHQYHEIQVSNVILRYICNYYYLVSSTFFCWLIEICIMNIGELYGIILGIIMFIEIASIIIFSDIVYQSGLILRVSTQLPLNNNYVKYFIKPLRIFQIIIFFFLTNQKLGLLLQNVLIITIQILHIYQILELKIYAKLKCVLASIIIASFGIIISIESLIMQNRINDNLWLLLSLIMIYLFQFMHKGQCLSLQSVDLRRLKFLFSYVEGLDGNHNNNKLKMTLIVCEHQQICTKIECICKKDMNIKQKILQLIEMKFELLLEQRTKKIEQLTLDLSQILFRRRQYVKAQQVIFRVAYQKDNYQNKYNSFNLSLTTLCLLDFLKYQIENDVLIQMMERMILTNPNNKLIADSLKQFSINESSDQYIQTKLKKTILDKSLFYYYFIEHSNKFQGDVNYPIKIIGQLQQFRIELTERYQKFPTSSNQNILKFYLLEVLNDYIEAFDLNKNKALNDEKYQLFHDSVYNKLFGQNPSYIVAEINKDLDVIIVRQSKKAMKKFQQIYGSKWKNSLDISVIDFIPEYVQKQHRTLIEQFFDEGTNKYYQQQNLSFLKLFDKIMLPIEMMVGLNQSSSQTFSFLIFFYEQMDYRSYLVVNQSFDIINCSENISSQILGNSQYSTSFISTKNLGTIFNIIPEFDLMIQSNVKNFFNHNIRLLSCNTKQASQRFLDYISNIIIEKKFTNENMCYMITLDNIRSNNNRNHGDSRLDTSIFQKQQTSDLLMIHKLDKMKEDIEEDIEKAFPYQEIFDISLFKKGNPLNQSETQHVESKLYITDLIVDQEQTNPIHQKYSKANQKLFSPKESMNEEFITKKNFTFQNETRDFVSGSSITMVKKSKFYKKFEIIYSLCKSLKSSSKLIKANINFILFIVLIQIYVIINLSLISNIRGFIQDIKLLSVRYDVTGPLESFSVSRFTQINYREMLGRKVINSSYAAQLQIFPKHYLMESFDKMKKSMGEVLLRSEIDQISNITYLPIMLYVNMNNTGQEYNVSKRSVIIITLNYQYDFKLHLDGIGGGFDNPYYYFTYKDYQIVKSMFDNLNVIILDITLERSNQEKKKWMFGYFPFLGLNFLITLFIILQQIRHSNEKNAIMKYISSIDLNIIEQEKLRLSYLQQIVVQDMDVIKAFIIDIDQVDNQLEKSNFKQKSFFSKKSNTILINNKPILGLTLSIGHFLLFAAFSIYLFLTFRIYFFKYEKTAVFYQQLSDLGVDIPAVYAQKEILFRVKIRYPFLNQSDLNQTYAVIFNALNSISRYQQQNLNLSSTDYLFDEYLIDFFSMINTGNLCEFQSNELKQQTQSFCPQVMQGNLVLGFQQSLIYILNSVRVQQSDTQNFTVQPVSTLLELEGSTILTNIIEIMKEQFYNNLVVQAQNLILNQQLLCIFYLSFEIILAIFYFTKIDKYNYQQFLYLKQSVYLFPRHTLIFDDSFYRNFRSIVNEENLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V2S2</accession>
    <name>A0A8S1V2S2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0540174</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8171205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V2S2"/>
    <dbReference type="OrthoDB" id="302433at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="82"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8171205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="212" mass="24728" checksum="A48B82AFEB2A9620" modified="2022-10-12" version="1">MDLQHSKSYAALNQQNCQLFKPKPLSIRQQMAQLPTQTPRVQIQALTTPTTIECDSNPYLNQQLCSFKSYRISTEIINGSPLDQIQLILRENEKLKRSLNQKQQIIESLSRTRKVKPRLDFCDLKKNSRSLLQPSSQQESIKPKNIEVKQSISQNYNKRTKLPKIEDSIPTNEDDCNFTFANNFFNNNTCKNQKINFKQVFTQSHLKKKFFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V4W1</accession>
    <name>A0A8S1V4W1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0570190</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V4W1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8172628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="42" mass="4960" checksum="1BA22194B69C4A67" modified="2022-10-12" version="1">MDFGYLMIKSKPKNGLKNSQKLEKKQEIIFARSALEKIKQNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V7N1</accession>
    <name>A0A8S1V7N1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0580039</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000058">
        <property type="protein sequence ID" value="CAD8172777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V7N1"/>
    <dbReference type="OrthoDB" id="296906at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090114">
        <property type="term" value="P:COPII-coated vesicle budding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037363">
        <property type="entry name" value="Sec13/Seh1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11024">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN SEC13 / SEH1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11024:SF2">
        <property type="entry name" value="PROTEIN SEC13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="3 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="599"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="649"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="310"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8172777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="798" mass="92370" checksum="EE496FF56A42DBFC" modified="2022-10-12" version="1">MGCHVTKNVISDKKFREQAITDEMLQECKVDEDVKSRISVYNQALEKMQKIPKLRNTKEKCKNLKKTDLLYLYIKYIIKYINMQELKLIGKECPITRGGCNFLQYDKGKYIVIGGVNRSENGLQELNDFWTLHLPLKDGNLAGIWKEMTLENQQLYTPRSSQGACISDDKMVYIFGGQKFLESQSTDEFYRIDTAQKVLTIIKSKNKPRARNSHTLTYYQEKLFLFGGANDNGPLNDLHIYDIKSEVWQQIKDVSELIMAREMHTAHILQMNLKPLKKSECNKAQKLVGIFNEFQGESKEQIEQIAIKEQIIEQNNNEQQQQQIDEQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQENNQQDYLIILGGRNKNDLLQDIQVINLSTFEVEQIENLPTPLCAHTSVKVKQDIWIFGGCDGASLNSNIYQLQDQSIKKVGTIQTAIMASSALYSEELNKIIIFGGCTLDRDLNQTFVKTPKPEFLQIPSQTKSVNKINMIEVKNEGMIHDVCYDFFGTKIALGSSDGHIEIQEINVNSFKSEQQKDILSYKISDFQHKGPVWQLSWSNPKYGNILASCSFDKTICLFKEQKLGKYSLLLQLSEHQESVQSIQWSYRKLILAAGVADGSVHIYYRKKNGQIKNQQDWEHKSESQHQSCVNQVSWVRDKELDFATCSTDKTIRIWKVTTPIDGEYKLTQIKYIQCESWVRSIDWAAEQYYQDQILAAGLESTQVDIIYLKWDQNNDQQVNISKIVQIQAKGPCWKVQWSPLGNQLAISTLDDKNDPFVQVYRQTQQGEFVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VDM1</accession>
    <name>A0A8S1VDM1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0610227</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VDM1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8174681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="50" mass="5952" checksum="7744A232B77BAB09" modified="2022-10-12" version="1">MILNPKSFNFPKLTFHIYVQLETITSISIMKTEKNIRFSFIMFIPIFVVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VFR0</accession>
    <name>A0A8S1VFR0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Acyl-CoA dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0650203</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000065">
        <property type="protein sequence ID" value="CAD8176678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VFR0"/>
    <dbReference type="OrthoDB" id="434771at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050741">
        <property type="entry name" value="Acyl-CoA_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083:SF13">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083">
        <property type="entry name" value="MEDIUM-CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="6">
        <location>
            <begin position="401"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="5">
        <location>
            <begin position="485"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="4">
        <location>
            <begin position="601"/>
            <end position="748"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8176678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="769" mass="88775" checksum="2201168B9B674F9E" modified="2022-10-12" version="1">MSLQMEIDKLISYLNSQSSILDYRQNEFVKNEQVSFLPTGEILIICSKDPQNTMILRQQPIFLKLGITSLQEDLKLQSELLRHKQEVIHFCEDEKFLGSRFYVKGQIKGRVFNHLTIQSLPINERKLVFNEIISFLSHIHSFGQSKNIFNYKVMIESLRIQYKTNETRIQTNIEDLLYWLPMNIPELTIQESLCLGNLEPNNFIFDQQQIKIINIQNWNSVTIGNSQIELANFLSNYDVPYIKQVTDYGLQKIYNFLGLPNKADLINQYCIERKLDTIIHLKYNTIIALLQRAIFKQQCIKTKINPEKSNIFQQELEIISKCAWNIVLELTNDDPFQIKLRIETDKLQWSKYPVTQRCISYYYRIRDFLKDEVFPIEKSQFDIIYSFDIENHWKPLPGIVHLQKRAKSLGLWNLFISDPIYGKGLNNLEYTFLSELMGTSYFGHQIFNCYAPETGNIKLLIMAGTDQQKEKYLKPLLEGECKAFFAMTERNVASSDPTNFETTITKQGDGYIINGSKWMVTGIQDPRCRFGIIMGKTTVNSKSPFTEQTLFLVDTQHPNIKILKPLSVLGYDEAPNGLGEIIFDNVYISKEQMLGKEGAAFAMSQGRLLGGRLHHCVRLLGLTRRSIDTLLERGQRRYHGGKKQKEDTSYQEKIGKIECEYQICKTMILNAAMILDSLGGRHMQSLKAVSEVKVAIPNKCQWINDECIQLFGAQGITTDYILQIAFQASKSVRLMDGPCELHKKQVARFTQGSHMFNELVNVNGYIANL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VHM2</accession>
    <name>A0A8S1VHM2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0650216</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000065">
        <property type="protein sequence ID" value="CAD8176704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VHM2"/>
    <dbReference type="OrthoDB" id="438722at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051834">
        <property type="entry name" value="RING_finger_E3_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931:SF3">
        <property type="entry name" value="RING ZINC FINGER-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931">
        <property type="entry name" value="SI:CH211-59O9.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="92"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8176704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="138" mass="16343" checksum="547AF943BA3FDD0D" modified="2022-10-12" version="1">MDEGVFQIHLQMCEEQFKNQNLIEQECEQCGKIIIKFYINDHLDICEGNFWIQVKCPYCSLAFLKADLKNHISQCTTFQQQQIKEQQGVVNCTICLEDIEENKIQLDCSHFFHKDCINNWIQKQNKCPVCKKRHNIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VKE5</accession>
    <name>A0A8S1VKE5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0670068</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000067">
        <property type="protein sequence ID" value="CAD8177191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VKE5"/>
    <dbReference type="OrthoDB" id="303031at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="1">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8177191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="116" mass="13806" checksum="E14C7E0C8032A010" modified="2022-10-12" version="1">MYSISISNFYFNNSKVEYIVKFEGQVETFLSKYRYSQLKTLDDQLGKTKVEKPKFPKRSLFGKTNQNLSRVQDRQIKLNKYFEQLCVINSTMDCFIMQEFIRDAKRNIFVTEITGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VMR9</accession>
    <name>A0A8S1VMR9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0690223</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VMR9"/>
    <dbReference type="OrthoDB" id="303868at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8178490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="133" mass="15371" checksum="A5F8173570A0F6A5" modified="2022-10-12" version="1">MKGQKLNLKGQPIQEISIFFEKPIQISPPSTPEEIYTIQPKSSRSTLNNKNTNVLSSSSNSQRILQQNNSLHLPNFPKSISPKKLCDQYISPLRLKGYTYNDIESPIQLRLPNRRATKLCSKVLQKYKKKYYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VPZ8</accession>
    <name>A0A8S1VPZ8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0680050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000068">
        <property type="protein sequence ID" value="CAD8177722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VPZ8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035946357" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8177722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="325" mass="37944" checksum="22C15A4AA29E6D30" modified="2022-10-12" version="1">MKIHVILQLFVLCKCIKFSLTVRCACNEYDEEYCISRNYCSWNGSSCIDLQCQYQKEEYCNTDMTEYQCKWNETLKVCQSYSYQCSELKMDECQNSMYYLDCFWFINQCVPHECKDHIDCNPDQCSSSPQGCAHKVDNLNCSSYTQDICIKYDGYGSGCAYSIMNGCFDLRSSCQDYSESISFCNTMCYYDIHSQQCIPQECSQITVKSKCKSFISEIPNQQFIPCKWSNGQCKELDIEDYKDIDLNKCKIYSINSFWQDSTLTCKLCNSFVSHPKYNQKQRKNVTKNNNQSALNDTQDILENQQNQQNGIYILQLLLISYVFII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VUT7</accession>
    <name>A0A8S1VUT7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0730104</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000073">
        <property type="protein sequence ID" value="CAD8180003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VUT7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8180003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="640" mass="75334" checksum="13C184A3FA77A2BC" modified="2022-10-12" version="1">MSIIRENNKNRGRVYRCFAIFNCDRLEFTHIQKRRCNKSYESSYQDVRNQAENCYSIHSLELSYKNAYSEWFGILDQPQKCYFMFMLFPQTNIQLVNEALNQLIKLISSIPNYYHLTSEQLDQLKRAEIRKLIDKLENEYIEKEGIMGNPSEDEQAGSKIISFNPIKSNPPSSFRSNFQLQTSKNDTLIISQKELAQTKTVDKEYVEFGVYRGFAIFSLQLQKIIFQIRRGSKQAFTQSINNIEKLLKVNSKQPEQYSIFIEKVNPRAEWHGKYHKRDQCYYLIMTFQNADPEECNKTLKLAISRFSKDPHFVQYSQQELNDKYLWDVSNLLKSSERHYEYDQSHLGVPSDDEIIPKLDILKSSTLGLTKNYSSFSASKMELIQMQILPRSHLMQLGETDLDSPIQTKETILNKLDHFEPLVNDTEDILQYGTTAVLTTNTFKLTGNLKPLDKDIVQPYNIIVKSPKLNRCLLNWLILKNQQCKYFKLQFNLISINLENKHNEQHNQFKNFTNAKLTKLDSDIVNLDQAIEQEKLDRIRTEKENIENIKESIDFIYISKILLELFRNLELEKQTKVANEKKILNTIKDTSQSINQAIKNEAIEKEQKHQELIKILIIHYNLRIDIQKNLETIQLKNFIYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VZ00</accession>
    <name>A0A8S1VZ00_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0790202</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000079">
        <property type="protein sequence ID" value="CAD8182914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VZ00"/>
    <dbReference type="OrthoDB" id="302953at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14003">
        <property type="entry name" value="STKc_AMPK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:002041">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF82">
        <property type="entry name" value="KP78A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="7"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="377"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="514"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="336"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="377"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="404"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="532"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8182914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="737" mass="85837" checksum="306AB13BF9FE3D53" modified="2022-10-12" version="1">MTELGNYLIGKTIGEGTFGKVCHAKHQILGHEVAVKILEKNRIHDELDIERVKREIKILQMLHHPNVVQLYEMIETETHIYLFMEYAEGGELFDYIDQKKRINEVEACKFLHEIISAIQYIHQLKIVHRDLKPENLLLTAKKNILVVDFGLSNIYEETLKTACGSPCYAAPEMIQGKPYHGLQTDLWSCGVILYAMVCGYLPFEDNNTQVLYKKILTADFHIPRYVSLDGKDLIKNILTVDPNKRYNIEQIKTHKWWQLWKSDYVQVSPFKASFNQITYTLLPNSIYKPSLNSSLSQNITEVENTCCDIVQESNKIPLCEQQDSNNKLTIQEDTQQEKLIDQKELLVNDLDELQKDSEQQDLQDIYDYQQSVRSLTRQKSSRSIQSIKHLQTQVESPQKPTILQSNKQTTQISQTSQTKSTKPISKKPVLTQQQQQSIIQKKPNTKTVPSKSLHEPPNFENKFMKQTNTSNMRSTNTAYSNNNSVHISHQMKTEIPKKNSNPPPKLSAIQEQFKENKEKSHSLKTQPNSYKQQQQEQQLQQQQQLLNKTSNQLKSSIKQPTNLISSNKKTLHQKQAASVQIERQSFQIQQQIIEDNNIIQFEDSKTIVDTFTLRLNSYKSQVVQKQIQKQGLQDNNGIETFQGPYNLQFITCKHPKLFIKGLQKYIQEKYQLEFSAFQSVQYGFNFNLSEFSIQIKLFRIENLDIFYCQINCNYQKNEKNDNEYQILIQDLQTNFEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WDY5</accession>
    <name>A0A8S1WDY5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0880025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8186872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WDY5"/>
    <dbReference type="OrthoDB" id="303975at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8186872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="218" mass="25283" checksum="720A4DB513EE811E" modified="2022-10-12" version="1">MLNTNFNAYYVQQPFFYPFGYQYLLMTAPIALTNTQIVNPQIQVNDNLTQTNNISIGLNQQQGRQKQKTISETESHLDCDSDNDEPRDTAQNPKSIKANLTKNIQKLNLLVKSTNIQKNYAKAIVSYACRQRTIIFESLGEERGEEFIKLMNHLKNKLRNIGHITKYTHVEEFLSLFRVLGNNFMKKDSVSYIYNSKIMQKSCHLSNMTIVRNSLLKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WEC0</accession>
    <name>A0A8S1WEC0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0890031</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000089">
        <property type="protein sequence ID" value="CAD8187351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8187351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="153" mass="18311" checksum="5714E0A35630D5E4" modified="2022-10-12" version="1">MKNFQKLQTIMMAEILIKQILFRKYFSLQVQINLIQQKNLLXGSSKKIQTRSRGLESEGALRERLRGRDLLPPFGKQTDALKNNELSYDTLYLPNIIHNLFQLDKMNKKVIIKQRLKTKRIIFSFKLYSRYGKHRFILNMNLWKNQIIVIQIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WHA7</accession>
    <name>A0A8S1WHA7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPENT_87.1.T0900154</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-dependent RNA helicase required for 60S ribosomal subunit synthesis. Involved in efficient pre-rRNA processing, predominantly at site A3, which is necessary for the normal formation of 25S and 5.8S rRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8188007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WHA7"/>
    <dbReference type="OrthoDB" id="307056at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="90"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="308"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="471"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="512"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="674"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="874"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="525"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="535"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="558"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="695"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="792"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="808"/>
            <end position="838"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8188007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="908" mass="107204" checksum="906753703B298D67" modified="2022-10-12" version="1">MKGLKRWSQPLGMFASCPGEFEDFTKCFIDASNLQYSQEDIEKFRSDNNITIIRNGEQDNQIIQPFLDWRHFPLGPPEFQQPTAIQSEVIPIVLSGRNALAIAQTGSGKTLAYLLPALVHLEQHAMVKESTQPKLLILVPTRELGVQIYDQLLQLIEYYYGNKKQNDKENSPNLMSLKIVCIYGGNPNKKQQVELIQKGVHVIVATPGRLIELIEEGMVNLNKISMLILDEADRMLDMGFEPQVRDIVSTIREDRQTILLSATWPNEVQQLSKEFCYNPILVKIGKGAPITQKIICTGQKEKLHVLMNVLDDLIYTDKVLIFAETKKRCEDLSQSLTKQGYFCISLHGDKSQDQRDAIMKQFKDSNTRLICATDIASRGLDVKDITVVVNYDFPKSFDDYIHRIGRTGRAGAHGRSFSLLSYEKDEGILADQVITYLTSCNQEVNQELLEFKDKALKIKQERQHKKLQKYIDNSNQTNIPNSIYPPNNFVQTSEFKQDNSNQWENRQQYVNQGGNQKKPYHKHSRSEYNEENKGEQQQYQQQQQRNYNMDFKNKQQQDRQPQLQKNQGDSRQGNVRIRFSDADQTKTQDNNQELDLKNYKLLPQFQEEGKTNSFQNKRFNDQDQGQKMYNMDFRSENDNQNRRNNNNTQNNNHEAQFYNDKYLNQLKNEKPYHQKNYDKNFNNHNERPINTFDRNNNERITFDRSNNERNYVDKPNNERNYLDRNNNERYNNERFNNDRNDNERFNKEKNNNERFNNDRNNNERFINDRNNNERFNNEKNNNERQNNERNFKVNNNERNFNNNGEKNYSNQNNFDRSNNERNFNNPQASRNFTGGNDKSFNERNFVGNDKYQNNKQMNSIQNERQFNSQKNNFNEQNYENDQKIQSKQYQGYSKSDYTKYQNMKGQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WHX8</accession>
    <name>A0A8S1WHX8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0910072</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000091">
        <property type="protein sequence ID" value="CAD8188305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WHX8"/>
    <dbReference type="OrthoDB" id="292078at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8188305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="86" mass="9909" checksum="4558AEFE1D17DF5D" modified="2022-10-12" version="1">MGSSCCAKQQSEKEIQQGIKSNYIRSQTSNTSKVLFSKPSQEVKYESHILLNIDEEPVEELKEDFKDKKLSPLSKNLNKKFLNMLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WJK7</accession>
    <name>A0A8S1WJK7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0940139</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WJK7"/>
    <dbReference type="OrthoDB" id="336906at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF519">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE MPK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dual specificity phosphatase catalytic" evidence="5">
        <location>
            <begin position="160"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8189663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="303" mass="35915" checksum="FA382AD1E23FE702" modified="2022-10-12" version="1">MKAIQMMELYNMMNDYRHLLIADLRKLELFEEKQIRGSFCIQDNVQIIVSHIEKQNSNAQKNEEKYHTQPIRRIVIVNDDYLKIEQDTQLIKDLIQSLKMNEYKVYYLKTSVETFLDKYPFYILSKVILQNYENLHNNEDQQKLLFAYSQFPQQLQENKLYLGSNLNANSKKQLDALQIKTIIDFKTYEPDAKFSVWKKEQYNYINYPISEEGEQLIDFYQITDLIESQNTPILLCCPDGYSITNVVAIGYLMTLKKQNFNISSLKVFQIRGNTTVNKKLYCQILTYDPKTIVNKITQQLVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WK62</accession>
    <name>A0A8S1WK62_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glutaredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0930058</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000093">
        <property type="protein sequence ID" value="CAD8189150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WK62"/>
    <dbReference type="OrthoDB" id="9932926at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8189150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="168" mass="20137" checksum="E8A7F793B8A99CBB" modified="2022-10-12" version="1">MDQQPQVELDEEQQLENEIKNMSAYSIQLVWKKYKTLKAIRQTVKSKREPLVVYYSSIATNQKIRSDQMQLFHMLKVKNIRFFKFDLVDNKMAQGWVKDILNRYQLPFITINNIFVGGYSEFEILYELELLPRIIKKDYEQMCILCDEPKVGDECPNCKKPYDFFKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WN68</accession>
    <name>A0A8S1WN68_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0990040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000099">
        <property type="protein sequence ID" value="CAD8191458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WN68"/>
    <dbReference type="OrthoDB" id="308466at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8191458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="211" mass="24775" checksum="EB93568F4B8D6021" modified="2022-10-12" version="1">MARVKNGKQYQKQNPQQIQQQQQPVQQQPIQKNIKKIKQAPSEERIIERKPSPNLLLEKRTRRELQQQSVKKKIRQPYNQGYYPDVPNLQFPNQLIDCLIKVICSFGNSAKFNQIIKNLSLFYTNEIQEEVVNLVLAESEETGAIYELEGKFKVTNALIQKPLLLKNDQIGSKMKHEDIDDDNDQFHSLSNNHAQQDQEIEDELNQFRQYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WT96</accession>
    <name>A0A8S1WT96_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1030095</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000103">
        <property type="protein sequence ID" value="CAD8193078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WT96"/>
    <dbReference type="OrthoDB" id="306198at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00052">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216:SF174">
        <property type="entry name" value="GH06923P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00027">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50031">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EH" evidence="3">
        <location>
            <begin position="8"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="EH" evidence="3">
        <location>
            <begin position="171"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="264"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="320"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="264"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8193078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="453" mass="52882" checksum="13DA6C17A025DE86" modified="2022-10-12" version="1">MFQYTPQEETYYRQLWAPFDPQGLGFAEAKGTVQFFKKSRLPVDTLKRIWIAGVKSQEGKLYYSEFATVMRLIAYCQNGFEFNEQLLNQNSPVPLPVMEGIQIPQQQQQPQQQLIQQSQQQQFNNFQQAQNQLPQSNYFQTQPVPNNVQISLQSYLEPKLEFSKYDLPDDQYNKYELCFTQQDKAHQGSIQGDQALAFFQKSQLPASTLNQLWSLVDIGQKGFLFKNEFIVAVHLIALCRKDTPLPQVLPESLLQLIRRDQQQNQVRSTSQTMPQIISGLPPQPQRAGSQNELQGSNLQTSYQQNKIQNQTQQIQFANNVDQQKQIIEDKKKDVEFLNQTIQKLQSFYDFLKKEQDELQAQITSLNDQQTQKQHQMNILQDSILHQLQVNQNLQKQLYELKQTNQVQENQTPTQTTQFQTIHIEDDYDYSNYRAKKDKVVSGGNQNTENFPWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WU55</accession>
    <name>A0A8S1WU55_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0930126</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000093">
        <property type="protein sequence ID" value="CAD8189286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WU55"/>
    <dbReference type="OrthoDB" id="288483at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8189286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="281" mass="32542" checksum="7AC2085C6E688B85" modified="2022-10-12" version="1">MSGLFKNYSKFVSASVFASANKLIINPVRWTFNSANLFYNPSYRYTGLPQGHELTAFSLAQIASALTYEAQQFNTVDALKDEAQLNYQFSAQCVREAIKFIKNNNWDQEVETSAQLLYVIQNNGLLKDVNKEILLSNLSQNAKYLSTQNIVDVVISLQDHKDAGFWSQFFSGIKNKEFSWELQPIDYHGWDTMQFDYLDQVKNSFKLSNTWAVLTAQDSQLKRFVLEGVLFGYTNLWLPLLYREKRIKYVFDDRLIDRSAFKNALKKINEVHNVQEFLGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WUQ0</accession>
    <name>A0A8S1WUQ0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA/RNA-binding protein Alba-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1000040</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000100">
        <property type="protein sequence ID" value="CAD8191849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WUQ0"/>
    <dbReference type="OrthoDB" id="1699369at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="entry name" value="Alba-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014560">
        <property type="entry name" value="UCP030333_Alba"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31947">
        <property type="entry name" value="DNA/RNA-BINDING PROTEIN ALBA 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31947:SF36">
        <property type="entry name" value="DNA_RNA-BINDING PROTEIN ALBA-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82704">
        <property type="entry name" value="AlbA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DNA/RNA-binding protein Alba-like" evidence="2">
        <location>
            <begin position="18"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8191849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="106" mass="12019" checksum="3C61720C8EFE0D8B" modified="2022-10-12" version="1">MDQVKKNPIDPKDKVFQLRGSAKPRTSFFVSKIFLKKFDEVEIHALGDAISGAVRCAETLQRQGLATIQKIETLTQTLERGQKVKIIVSLRVTPDGKRRINEEIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WXN2</accession>
    <name>A0A8S1WXN2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Poly(A) RNA polymerase mitochondrial-like central palm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1070175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000107">
        <property type="protein sequence ID" value="CAD8194814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WXN2"/>
    <dbReference type="OrthoDB" id="420381at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271">
        <property type="entry name" value="POLY A POLYMERASE CID PAP -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271:SF40">
        <property type="entry name" value="POLY(A) RNA POLYMERASE GLD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="1">
        <location>
            <begin position="10"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8194814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="359" mass="42480" checksum="D07AF78A701049D7" modified="2022-10-12" version="1">MIQQNEESIDDYLIKLYNEKKMSEEIKEKIMKSIEKFVKLLKQINYKSLNPPLSKLYHSYFGSLLNGFGTQSSDVDYTILTNSYVNEKQFLQFTKQEIEKIIKSNKTYQRFVIERYVDSARIPVINIKDTIENVEFDLCVNNILGVINTKLLNQYANLHEYVKIGGILLKIWGKAQKLINQNDFSSYQIILMWISFLQREHKLPVLQDQEYLRQRNDQSGKLQIARAKPGDDIQTASDRFTTDVSFLTDPEIIKQKMEQCTIGSSKISELLRDFWKFYQYPDGKFFKDEYIISIHLPNHQLQKKWATNNKFLTKDPFDISHDPGQRVKNIEERRLEIFEKSKIIMQKQLLQQVKDIFSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WZE9</accession>
    <name>A0A8S1WZE9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1040141</name>
        <name evidence="2" type="ORF">PPENT_87.1.T1040142</name>
        <name evidence="3" type="ORF">PPENT_87.1.T1040144</name>
        <name evidence="4" type="ORF">PPENT_87.1.T1040146</name>
        <name evidence="5" type="ORF">PPENT_87.1.T1040148</name>
    </gene>
    <organism evidence="1 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WZE9"/>
    <dbReference type="OrthoDB" id="304987at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12621">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN CHORD -CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12621:SF7">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8193549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8193551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8193555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8193558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8193560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="526" mass="61592" checksum="22151081B2D91899" modified="2022-10-12" version="1">MFYFLQSIDQFGVQQKLQIPPINPTQKSALGGLVTLTLYGVSLGYFLFQFVDWQQNNKLPKVTSLNQQINNDQTLKFQGVFAEISYFQELSNQIDPFDPQNMILQPQLIITTSYFEETTINATFQYRKRQDGKTVNTLLIEDIEIKNSPIGTLDHKYINYQLSFGYCNPQLLQNGQNCADQNLINQFFLQDNYYQILIYLEQFDPKSKQIVKIPREFIIEMIKKSLFFNQFMLKAGDLIMDDGFLFPNSNKQSYFSELSILTTQFDQEHAKKVYGEELISILYFNLDQIKIVNMVEYPKISEILADTGSIISWILSISFIVSKYNENLSLEKTQREIITMYYNDFNDFQIQKNCLGKVQRVNYKGKEYDLQKSEEILNRLDRIAIEKMNYLNLQNEVAKLQLILQEHLGLQQIKKYLESKFTLESLFDKLGIPEKISQQEINQIHAQHKLQQDRIQQFNIQVASDSKTNKQIVEQEVELRIGLLDIKSHEYGQVREQNSNLDIQESLQHINNYQTVINVDKTDGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X283</accession>
    <name>A0A8S1X283_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0040537</name>
        <name evidence="2" type="ORF">PPENT_87.1.T1120128</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8136010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000112">
        <property type="protein sequence ID" value="CAD8196438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X283"/>
    <dbReference type="OrthoDB" id="309602at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8136010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8196438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="204" mass="24902" checksum="4D7BC13E1FFB0944" modified="2022-10-12" version="1">MANSQEKMQQDYIWIRDQSTGDADVKMRTFGQHYLYYHAPNKRERLEMIWRSMGKAYDWEMEKFRMQKKFIDRGNKRRFFKNFFRFIKNPFGYIYWKTYRIRQPKGRIITTMLGLGVIGTLYKYKLESNQIQKREYYLLTAGKNSEGSGLINTGYNNDKLARQGMPLTQMFYSYLLAKDIVVSRSRDQNYRKYFEMRKKYQIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X3F0</accession>
    <name>A0A8S1X3F0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">arginine--tRNA ligase</fullName>
            <ecNumber evidence="4">6.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Arginyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPENT_87.1.T1020157</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>tRNA(Arg) + L-arginine + ATP = L-arginyl-tRNA(Arg) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20301"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.19"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.19" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDO010000102">
        <property type="protein sequence ID" value="CAD8192766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X3F0"/>
    <dbReference type="OrthoDB" id="68056at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00671">
        <property type="entry name" value="ArgRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000116">
        <property type="entry name" value="Arginine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000006">
        <property type="entry name" value="Arginyl-tRNA synthetase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00123">
        <property type="entry name" value="Arg_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001278">
        <property type="entry name" value="Arg-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005148">
        <property type="entry name" value="Arg-tRNA-synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035684">
        <property type="entry name" value="ArgRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00456">
        <property type="entry name" value="argS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956:SF5">
        <property type="entry name" value="ARGININE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956">
        <property type="entry name" value="ARGINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03485">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00750">
        <property type="entry name" value="tRNA-synt_1d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00836">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DALR anticodon binding" evidence="14">
        <location>
            <begin position="485"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8192766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="601" mass="69468" checksum="FBEF8B9CDF9E6B0F" modified="2022-10-12" version="1">MDQIGVNTMRSVQKLIKSAIKTRVLTAFPNLPQNNFEIQLETISNNLNYEYKTPTVIRIFNQFKKQNLFGDLKSEVEVAQKIASTECQDNVIQKVEAIEKGYLTIQLNNQFIEDEINAILINGLKFPLEKKQKVVVDFSSPNIAKEMHVGHLRSTILGESLCRILEFQGQEVVRVNHIGDWGTQFGMLINHLLEEYPDYQTNRPALKELEDLYKEAKKKFDSNEEFKVKSQQYVVKLQALDPVCIDAWKMICELSRQEYNKIYQRLNVKITEYGESYYNPILPEIVKECEAKGIVELDKGAKIIRVKGEKEVPLMIVKSDGGYNYDTTDMAAAKTRILEWKCDRLLYLTDVGQSSHFKLIFEGAKLMGWHQPPITSMEHMGFGLVLGPDGKKFKTRSGDTVKLIDLLDQAKERALKQIKQRVQENQQGKEFATGTALSPEEFDTAAERMGIAAIKYYDLKQSRISDYNFDYDKMLDPKGNTAVYLMYSYVRMLSILRKSGIQDFEVYKTENKFKITHPHERHLATQLLRFVDVLQSVTDQLAINWLCDYIYDICVKIAEAYNQYRILNDEHTQTRLLLCEAIRMVLLQSFYLVGIEPIEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X3G8</accession>
    <name>A0A8S1X3G8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="27">Dynein heavy chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">PPENT_87.1.T1090156</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000109">
        <property type="protein sequence ID" value="CAD8195511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5593012at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.710:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.30:FF:000002">
        <property type="entry name" value="Dynein axonemal heavy chain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1220:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.180.20:FF:000003">
        <property type="entry name" value="Dynein heavy chain 12, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.100:FF:000001">
        <property type="entry name" value="dynein heavy chain 17, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1120:FF:000001">
        <property type="entry name" value="dynein heavy chain 2, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.20:FF:000001">
        <property type="entry name" value="dynein heavy chain 2, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000063">
        <property type="entry name" value="dynein heavy chain 6, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.720:FF:000001">
        <property type="entry name" value="dynein heavy chain 7, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.280:FF:000001">
        <property type="entry name" value="dynein heavy chain 7, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.490.20:FF:000001">
        <property type="entry name" value="dynein heavy chain 7, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000049">
        <property type="entry name" value="Dynein, axonemal, heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000362">
        <property type="entry name" value="Dynein, axonemal, heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041589">
        <property type="entry name" value="DNAH3_AAA_lid_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46961:SF8">
        <property type="entry name" value="DYNEIN AXONEMAL HEAVY CHAIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46961">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 1, AXONEMAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17857">
        <property type="entry name" value="AAA_lid_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0243">Dynein</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Dynein heavy chain linker" evidence="16">
        <location>
            <begin position="994"/>
            <end position="1406"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain hydrolytic ATP-binding dynein motor region" evidence="17">
        <location>
            <begin position="1539"/>
            <end position="1864"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA 5 extension" evidence="21">
        <location>
            <begin position="2027"/>
            <end position="2173"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain 3 AAA+ lid" evidence="22">
        <location>
            <begin position="2398"/>
            <end position="2489"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA module D4" evidence="19">
        <location>
            <begin position="2554"/>
            <end position="2804"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain coiled coil stalk" evidence="18">
        <location>
            <begin position="2818"/>
            <end position="3168"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain ATP-binding dynein motor region" evidence="20">
        <location>
            <begin position="3197"/>
            <end position="3424"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain region D6 P-loop" evidence="15">
        <location>
            <begin position="3669"/>
            <end position="3783"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA lid" evidence="23">
        <location>
            <begin position="3815"/>
            <end position="3954"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain C-terminal" evidence="24">
        <location>
            <begin position="3962"/>
            <end position="4268"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="920"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="2833"/>
            <end position="2881"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="3045"/>
            <end position="3114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF12774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF12777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF12780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF12781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF17852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF17857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF18198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF18199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="CAD8195511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="27">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="4272" mass="495931" checksum="4299B9A53E8F8E8D" modified="2022-10-12" version="1">[encoded data, 4272 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X4F8</accession>
    <name>A0A8S1X4F8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1030149</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000103">
        <property type="protein sequence ID" value="CAD8193166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="292058at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332:SF175">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1135"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1228"/>
            <end position="1253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1305"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1338"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1393"/>
            <end position="1411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1417"/>
            <end position="1435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1447"/>
            <end position="1467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1482"/>
            <end position="1504"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="93"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="196"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="230"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="272"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="365"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="395"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="441"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="497"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="537"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="592"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="634"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="664"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="715"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="863"/>
            <end position="897"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8193166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1558" mass="175038" checksum="65DEEEBA52F0E3CE" modified="2022-10-12" version="1">MQKRGTFLIGILPLSCPDGQYFTAGGGGCSNLCLPICTTCSDGTSCDSCSVSNPYLLLPSRVCTTCPTGCLTCDLSSCLSCDTGYYQSTLCIQCPTECSLCSSSTICSACNSGYYLSGSSCLSCTLPSVHCGSPCNCSSGSYLVGSCCYPCQAPCSECSGSATFCTSCVDSTNQSTSTCDCTLPTKYLNALNNCSDCASPCVNCSSLSSCSSCINTYYLQGTSCQACTSPCVNCNTSSTDCTSCIDSAHQTTPTCACVSGYIKNTSNDMCTACTFPCATCQNTIIECHSCASTYQYDSSAHTCTCLTNQYEDNGSPKECQNCSLPCTKCTSPTVCTECNDVSHQVLNDCHCIDTYYMDSSSVCQLCISPCLNCSSNSVCTSCINNYYLDSPNCLQCSLPCYNCVDIDTKCTSCAHSHQTVQSDLCVCDDGYYMDGSYYCQLCTHPCSKCTSTSTYCTDCASTFISSGSNTCVCDDGYYEETLNSPSDCQPCTSPCVKCEIESTHCITCIDINQTVDPSTYQCLCNIGWVANGNLCDQCQLPCTKCITTQDTCTECLDPNHLIINNKCVCKSGYGQSGLNSNYCSICEYPCLECSINVNTCTKCIDSTLFTFILLNNECICDLGYFLNNENNCIACIEFCLECIDQFSCEVCIDGYYYESTLCNKCSNNCKTCSNQNDFCLSCNNNYNLINNQCICGLGYYLQNDTCQKCKYPCIECSNDTTCIQCAQIDKLSMSDQQTCICQDGYYWSISQCLLCHLNCLTCQETSTKCLSCDLSLNKRLQNNQCNCILNYYLSDQNTCNSCDSEQGKVIQSCKYKNCNDQVWTYGEDCDDGNQIQGDGCSNCKIDKNYICTNTIMQKSLCYSCPQFCISCQQNSITNQNECVKCQSGYYLDQNICQICDKQCKECETNPSNCTNCRFIQSASKKCKLCEYKQGYYSDYSTNTCYNKCGDSIIADSEQCDDGNQINGDGCNNKCLMEKNFICLNDTCITPNYPIPKLSIFGSPQRYEKERKFKLEYNVPLLIVKSNFELSSELKFYIENRSGDVQLLNYPYTFIQNMTLVENSYYSYEAIITLQLNQSSQYQIFNIYFLDPSKIQSYQGYEQKIDHVKAAVEEYVYVDESTQSLTETFNSLSINLFYIILILLAVSILFGGLDIFYNLLDTIQLLSYLKYINLQFPYNLQTYFEIFGFAQLSFIQNYLNIEDFFSNYISLEELKPLPKKIQDDNYSSIYLVNVSQILVVYGTLFGLYSMAIIIPLTIKQVRFKYYEENPEKQAFTLKLKIYFLSIKIFIGEMCKKVVNELFYSGILRTFMATAYDYSFIMVLQIYTIDINSNSFLLQFSSLISLLAFILYIIICLVIIYLLGKNKLSLKQNNNIEKFGSIIEGIKIKNNYQKYFNVVLLVKKLMFMVILIFCYEDPLSQTVNLFLLSITTALYLFHHKPNEDKNEYIKQLSTEINLCLTYIFIIFLIVNDQIKKLNNKEQSYIGWICIVLITSIILIQLIIDLIQQWRMLLKKFATLRRFINKISNMFKKKAESTTPDKNVFEEIQNHEFYQSIILYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X9E5</accession>
    <name>A0A8S1X9E5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1160064</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TMEM104 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000116">
        <property type="protein sequence ID" value="CAD8197690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X9E5"/>
    <dbReference type="OrthoDB" id="294541at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16189:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16189">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 104-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="262"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="307"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="378"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="422"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="8">
        <location>
            <begin position="107"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8197690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="449" mass="50763" checksum="7FE29E5D6BC15653" modified="2022-10-12" version="1">MQQQKYIKPFPAFNTFINVLMASGPILLPISSVKVGYGYTSISLSICCLISIIACDFIVEALYLADQIDKDTNLIDRLNIQASHTVSDSTEEISSQPVQSKYALQSQFEYGSMAKLFYGKWAEYLTVSIIVIYLYGAVVAKGVMVGNSLSEVFQNITLVEQYWFWIIIFFALCGILSFKDVASIQIVQSTVAFARYLTILAMLGGSIQYMIQEEGTQIQEMQWFNIDALPQMISTAIFAFLMHHSSPGMLRPVRPTSAIRKIIFISFAFGLFTFLFVCLTATLAFPKEDFADASFYNKLFNQKQLKWAYYIISFYMFLNIAALPVLTITIRKNLMKLVAPHLIPKDNLQITLPTALFTLLIILPCATLAILLKKQIEMIVGITGGVCGVFILLTIPAALVLQGRKIHKVKYIDNPYISKFQHPFWIWILIVLGCVFVPYNLYMQIKKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XBS3</accession>
    <name>A0A8S1XBS3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1180130</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000118">
        <property type="protein sequence ID" value="CAD8198428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XBS3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8198428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="69" mass="7871" checksum="7E8DDDA01FB6385A" modified="2022-10-12" version="1">MKNMDLSDIRAKISELEQRQNQVQCSLNSTFFQGAGNVPLSPLSSTDLPWWVEVHNVVIEEEILNYPPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XGF2</accession>
    <name>A0A8S1XGF2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1240066</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000124">
        <property type="protein sequence ID" value="CAD8200330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XGF2"/>
    <dbReference type="OrthoDB" id="301153at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18849:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 410-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18849">
        <property type="entry name" value="LEUCINE RICH REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8200330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="543" mass="64276" checksum="CD028669F51DC995" modified="2022-10-12" version="1">MLSEIINQKQQYIKSDTITLSDFGIQTIGYSNTFKNITKLHLTGNKIVKLDGLEQFTNLQYLNLSNNQIESIKEFQHVPQSLQCLFVAGNPFTANLNYGYSLMCILMNLQQLDNDKINRQLTQEYQIKMQTYGFHVLAYFVHFNNKQLTPIFNNRSINYIQKLLMIIDQLENQNKDEIQGLCQQLQNLQFKKSIADIILKHICKFERDQFKSNNINVFFANQAMQSQHTDLQKFADDEGYYQQEVLQAFISYEDNQQFPTFSLNQAYNLGILDFIYQSTLTQNTTFRPQEDRIKQYVEPPQQNFSLINKSNASVQKSILKPTSYSVSTQSKFDECTQEYLNNYKKQYPFLKGFEQLKKSLQKYYLQEFKLKLKKKINRLNQQCIAGTFKLDYFKKLYQRYLIKIALQQINRKSKISNMINILQIQRKHQQLLSIKHIKSVVYVQKNMANLQRQIYLKRAVFKYLNPKIRYGKIAKKLQLKLLILKINGILREALKQIKITALTFKVEIRKDLAFQVTLKKEKKQESRALENFRKLRELGNTQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XH08</accession>
    <name>A0A8S1XH08_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Insertion element IS150 protein InsJ-like helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1230121</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000123">
        <property type="protein sequence ID" value="CAD8200099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XH08"/>
    <dbReference type="OrthoDB" id="294423at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055247">
        <property type="entry name" value="InsJ-like_HTH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13518">
        <property type="entry name" value="HTH_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Insertion element IS150 protein InsJ-like helix-turn-helix" evidence="2">
        <location>
            <begin position="40"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8200099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="192" mass="22076" checksum="48A87F6363452146" modified="2022-10-12" version="1">MIHSEEESVNSFTPESQDQNPEEHKNKDSKKYAIIDQSIRIQLLRRILSKQATIKDAAKEFGVNFSTAKAILQTYRKEGRIGKKKTRERNKNKQESGENSSPRKIQSMYNLEQPQQMRAISPEQKPQAPQIQPIFPVMSSPNLDNTGAQIALALCQRELAQQKLINFQLLMMIQNFKNISQLQVKEEPNIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XIT1</accession>
    <name>A0A8S1XIT1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1250056</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000125">
        <property type="protein sequence ID" value="CAD8200638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XIT1"/>
    <dbReference type="OrthoDB" id="301011at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8200638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="254" mass="30215" checksum="B1EBD1AC6F8B50A3" modified="2022-10-12" version="1">MQSFSVKIQHKSEFKLCSIINNQISNQELECLQLSHTLQSPISGNSQSMTENCCFTKINEKIIEEPLLDIQCEQIYSELDNIEYCRQTLVFTNMNELQQAIQKIKYIQNRFLMPINDIQVDKQPDETYKLGIITKKFINHNFRINIRMLSKFILKAIQFDLSFSSNDIIQYYDDSFHINPISLLVYNEKQTCYEALREMLNQVDLCQDIEIILEMLKDFHNQDLEQLINYNIVDKDIYKLKLQLINLIYKRMQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XJ39</accession>
    <name>A0A8S1XJ39_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1260045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000126">
        <property type="protein sequence ID" value="CAD8200897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XJ39"/>
    <dbReference type="OrthoDB" id="298995at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8200897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="126" mass="13971" checksum="41175D8FDC392490" modified="2022-10-12" version="1">MGLSSRIGRLGIFIFFVGFAIHQLQNPQIAIKQTHEKTNQFLQLISQLTKSPSIQFSLDSIPLVVFSCALLKFLVGLGVLLKWNYVKLIGSIYLLSAIFVVHNPILSQSYKDSIMFVGALGALQFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XKX7</accession>
    <name>A0A8S1XKX7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1290017</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000129">
        <property type="protein sequence ID" value="CAD8201771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XKX7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="190"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="266"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="619"/>
            <end position="685"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8201771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="798" mass="95294" checksum="26D89BA80135D79E" modified="2022-10-12" version="1">MNNEKNNKKRDKTCNDVKSLLTQPSMLFNQKVEDNIIHVIQQLQPLRTENDELKNTILATQTYLEQQRENLSQIIRETESRELIKRIADFPTLISILQEDMKSMQETLWKQSQLLEQQTNQLLILNEERNSNYEQIKMEVSKQSIQQQNIITHQDELIKQKDQTIYQLNQLIEDENKKIKKNQNSHFELVDSYNEKLEELTNQLNEEIEKQAQLKIKYTQMLQQQQEEHQTQVAIKQDQYEFALGELRNTYEKRMDKLIKDSEFRISEANKTYIRTNKELELVRNKYEQEIQKNEQQIILIKNQQIQLQQQQQQTHQLRAEMQQQSLYLHQTQAIIANLKKNNNIVLNEEQKLSEHKIQQSLDNFYLTNPTNHFSTQTQFQQEIEHQQQQTSPILQPEDINQISPNVFKLNLKYADQQTTQISPISNFKLNLEKIPHLELDEIPESKKQTNLIKKLKKRRNQRSKNREEQQYYYRINTNEVYTPNEFITGPSPKQSEETPHSKITSVNFTQAFATHSNLRRKSKLQINFSNNQIQENVINQEIQTQDISCQTEDFLLEYLIINENQKTINTHPQERSNSYYFSPKKKNQITTTMLDNSSSNQHQFSLQILQSDQDEFQVQQQKDEVSILKEQITVLQQQNYMQAKNITDLQEKAQQMQERMQENVKQMKQQIQRSSQENETLLKQLEKPKEVIKKKIELQKHLENQITQSENLQYQLIKATEIIKYFNNELENYKQMYNIFGQGTKSERLQTKKLPSIYQSSPKRKIGDGIQRRQSSMYEEAQKLIDNLYDIKPYKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XQ41</accession>
    <name>A0A8S1XQ41_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RanBP2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1320054</name>
        <name evidence="10" type="ORF">PPENT_87.1.T1320055</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000132">
        <property type="protein sequence ID" value="CAD8202806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000132">
        <property type="protein sequence ID" value="CAD8202808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XQ41"/>
    <dbReference type="OrthoDB" id="282337at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034870">
        <property type="entry name" value="TET_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23238">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="8">
        <location>
            <begin position="13"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8202806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8202808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="91" mass="10616" checksum="BBFDA3AC42EBCA9F" modified="2022-10-12" version="1">MNNSPYIVKRKQRNGDWVCGICKNLNFSFRNTCNRCNKPPTKTPSKHQYRGFQTLVLIHYEEPTESIDELLENQIDPNVTSSLLVLDLDQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XTV8</accession>
    <name>A0A8S1XTV8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1340087</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000134">
        <property type="protein sequence ID" value="CAD8203614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XTV8"/>
    <dbReference type="OrthoDB" id="311825at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8203614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="140" mass="16245" checksum="E68599CD041B92D0" modified="2022-10-12" version="1">MTKVVNYKVNGSGRDTYINYGNGGFYKSDSLHTEESFYQLSKQNSLPIINSKFKLYHTDGTGRDSYISESQGGFYSYQPKLNFLGRLRHYDPSPEAIRENDFLRRSQLVKPAKEQERQLKQSAVVEQVTQRLQLPKIKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XWM7</accession>
    <name>A0A8S1XWM7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1420079</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000142">
        <property type="protein sequence ID" value="CAD8205946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XWM7"/>
    <dbReference type="OrthoDB" id="284854at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8205946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="715" mass="83966" checksum="E00C7152EEE87E27" modified="2022-10-12" version="1">MGANDSKPIYKLYEHFKQINVHNNDFPHCQILQHKRDPNQQLILRTISMTDEAYFKKTVNQYQIRQKINHPNILNLSNYFYEFEQQLCGQFYKVNLLFEYPQSNLERVQIMNENQLIEYLKQAIFGLACMQKNEIPHNTLQLKYLYLMDGIVKVSDPQYFQQNTNYVQILQNPNCMESIYLSPNLVNTIRKNNWQPKHNQYKSDLFTLGMLFLQLGLNQVNNDCYNYIQGKFLEEQLSIKLQKLRIQHSQVFCDLIQMMLITQEDQRPDIIQMEQIINSNQFTGKIYQQQPNIIPKQIIPIQPIGLIDTIHQPSTNSLQLEQFPYNVQINQSKRINQVQNISPIKYTHQFTIKTTQSQRSSTPISKVMIPTQQVQQQIRDSSVPKQQTQIQNISQRQINHEYLNNYSKISIQNNTTITPTTDQSILSNKSLLQQINLPPKLPVNQINQTNSKFQQINQSSESKILPIQVFSIQLDQCDSLSKFYPSELCSKHSQFDENLDSQGNDENDTQRQGNNEYSQILCDTTNLPQQFKNTLTKEFSLPYEISKNKDQKVNESEFVVEHYGNGSRYEGMKKNGMRHGQGRFYYQDGGLYDGEWKENKMHGEGTLYYGTGQPAYEGQWSEDQFHGFGTLYNEHPQYSEECFDYRDFDNVEDYWTKYSGSFKMDNKEGQGSLHLTNGERFVGCFERDLINGKGVFYRMDGKMMEGRWVDNKLVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XXG6</accession>
    <name>A0A8S1XXG6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1420132</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000142">
        <property type="protein sequence ID" value="CAD8206020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XXG6"/>
    <dbReference type="OrthoDB" id="310747at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF17">
        <property type="entry name" value="PROTEIN MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="130"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8206020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="150" mass="17942" checksum="191F4A1981089900" modified="2022-10-12" version="1">MKPYFDQTTQPQKLNLRRLGIAWLMGNVFMGPLFHYNFTYMLPWLVKRLPFDTTTPIRRAFGSVLIDQTVWSCYLLCHYLIIINFLESGSITKGIEAIKKNFTKVMITNWQVWPAAQIINFWLIPRPYQVLWVNLIGYFWNIYLSYIQHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XY02</accession>
    <name>A0A8S1XY02_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1440031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000144">
        <property type="protein sequence ID" value="CAD8206433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XY02"/>
    <dbReference type="OrthoDB" id="10308276at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8206433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="516" mass="60368" checksum="2D1660A75905E8C9" modified="2022-10-12" version="1">MQQQQFIQNFLKISKFKNIIRFLDHNYLSKYVISLQWTIYQITNSGNTIEIEDCSQQSIQLQILSIVTTIFGYIITIYLITNVINFGKSSDKYKISAELNENVQRYLHRDKIESINIEEFQQELQQELREELFMPLLYKLPFSKQFLQNLCQKLKSAVYSNGTILQFQNQMMDRLLFLMKGNVAQCIGNKILIHNQIPIQYFYCQIQCPIQLKCLSESLIAYIDLNDFLQLIKLHNSDFQKYCMLKDNYKIACHCCGYRNHTFHQCNHVFYVPNFKELILLHNTSEPNNRMNFYRDNGRNRINSLMNKNLICITAISFAIINQIALEVDLTNEVMGRFQGTISNFEDSILNDYQEERSSRQSLQQRLGTFDVPKSYNQGDTFSSQTYFQNSIPVLKVEEREASQNSRRYIHKHIDYSATNNSIKANLVKSSNSNELQVKRSQKIKQSNSSCSLNKKSGLGGSGISQLGSIIPQQEVYEDDLDQVCNYEYYYPSYNIEYIANALNHKLMSKYIQFVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XYH8</accession>
    <name>A0A8S1XYH8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1420119</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000142">
        <property type="protein sequence ID" value="CAD8206007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XYH8"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF271">
        <property type="entry name" value="MYB DNA BINDING PROTEIN_ TRANSCRIPTION FACTOR-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="2">
        <location>
            <begin position="142"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="142"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="198"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="2">
        <location>
            <begin position="203"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8206007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="338" mass="40549" checksum="F17FDD369C35AF84" modified="2022-10-12" version="1">MEDLVSPTHSIQIDCKFVTRLEKLKKLYDTSVQRKDQQHIILQQSNITINDWAEIQISYKELEKAHNEFNITNIGKQKKMDQLEFDLLRIIVGLYCNVHKLQPLQMNVYDWDQIGQILFNRHWKVCRSKWIESQATSIVDHPWTREEDQVLSQLYDKYTEQNKCNKWSLIAMEMSKLCNSSHVRLGKQCRERWINKLNPQVERGPWYKEDEIKLLVAVLKYGKKWSQISRRDFENLRTENCLKNRFHTIIKRESTRFEINQTQSKKQILQKLKQEDEPQLIEQIIQELREESLLQQQSDQSDKLKKKYTLTQADQVLDFVNNTFQIAIIKKNKLNIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-02-22" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y2M7</accession>
    <name>A0A8S1Y2M7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0420099</name>
        <name evidence="2" type="ORF">PPENT_87.1.T1420058</name>
        <name evidence="3" type="ORF">PPENT_87.1.T1420060</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000142">
        <property type="protein sequence ID" value="CAD8205904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000142">
        <property type="protein sequence ID" value="CAD8205908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8165214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8205904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8205908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="143" mass="17012" checksum="E1FBF84B9C04364D" modified="2022-10-12" version="1" fragment="single">MSFSTTCEFDHSKNIHRKFNTLHISSRKHIDPLVTTKPFETRNHGQQFEQHFAHYLKGDLNLQRLIQTLNYPRLNAEQQLQPKHRMTLKIRQQTEQRKNYLKSKIVKVRNLIKEKVAIKKPHNEEIRQGNAQTLTIVQTILXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y324</accession>
    <name>A0A8S1Y324_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1500137</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000150">
        <property type="protein sequence ID" value="CAD8208426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y324"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8208426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="231" mass="28258" checksum="B98117A97C018376" modified="2022-10-12" version="1">MEKKRLEFWNQFKIILFNQKQQKDIMIWLYKKEQRKFWICSQQLVGQTFSMLCFIYNQKKIQQYLGVTIRLQNFCATIQMAKITINNNRLFQTIRKIAVMFMHSLYMKIKLKQFYWNLITSFSLGNCQKLLTHIQGQYYKKQKLLVMDIVYVLQGMIFLPFNLDQSKKMNIYKITSNSEGQILKNKYIDIIGYSQSFDIKIFDQKMHSYDQEWQKCQFNKMQKLIQWKHNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y336</accession>
    <name>A0A8S1Y336_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1480037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000148">
        <property type="protein sequence ID" value="CAD8207637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y336"/>
    <dbReference type="OrthoDB" id="296752at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032727">
        <property type="entry name" value="CLAMP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28457:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 119"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28457">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8207637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="303" mass="35531" checksum="8C0D00DB6B9F8F39" modified="2022-10-12" version="1">MPPKNKKEEEAARLAEEERKRQIEEQLRQEEERKYGCGRYRTNMGLPISQYIINEVFQQEDASNLIKEYLCKILERNHCEKLRDIDIEIAGEQLINDFNACQDFGFNGDQALIFINMMFLVYINQHEKFGKLVEGGLTRGKSIQGDRKLFQQLLSQHSTDGQGCHKVFMAKQLQPIIDYIEKGYLSHWLLLHQAHSHNQRPLEIQVDLQIELPLPQPSLEDHTYYKPIVNDDTQSFKKTEMEEQEIEYHEPTDEELLDSVIMNAIQDKLSLAQQELETRLKDRQVEIESKLQEMAAALTKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y383</accession>
    <name>A0A8S1Y383_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">succinate dehydrogenase</fullName>
            <ecNumber evidence="6">1.3.5.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Iron-sulfur subunit of complex II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PPENT_87.1.T1480082</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a quinone + succinate = fumarate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:40523"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="6"/>
    <dbReference type="EMBL" id="CAJJDO010000148">
        <property type="protein sequence ID" value="CAD8207717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y383"/>
    <dbReference type="OrthoDB" id="294352at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1060.10:FF:000001">
        <property type="entry name" value="Succinate dehydrogenase iron-sulfur subunit SdhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050573">
        <property type="entry name" value="SDH/FRD_Iron-Sulfur"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004489">
        <property type="entry name" value="Succ_DH/fum_Rdtase_Fe-S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025192">
        <property type="entry name" value="Succ_DH/fum_Rdtase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00384">
        <property type="entry name" value="dhsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004616">
        <property type="entry name" value="PRK05950.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11921:SF29">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] IRON-SULFUR SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11921">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE IRON-SULFUR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13085">
        <property type="entry name" value="Fer2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13534">
        <property type="entry name" value="Fer4_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="16" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="8" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="14" id="KW-0408">Iron</keyword>
    <keyword evidence="15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="20">
        <location>
            <begin position="199"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00033304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAD8207717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="306" mass="35616" checksum="338164FD4E6285C6" modified="2022-10-12" version="1">MLQRLSAVIRRPIFWHGTHHHHDSNDATKRLFDTVSSTVKGIQQINYVVEHNPKLTAEEKSKMKQFLIYRYDPADENDFPKYVSYYVDLKKIPPMYLDALLYIKDNYDSSLSLRRSCREGICGSCSMNCNGLHKLACIHAIDTDLTQPAYITPLGHMFVVKDLVVDMTNFYTQYKTIDPYLKRKTPKEGNKEYIQSVEDRKLLDGLYECVLCACCSTSCPSYWWHPDRYLGPAVLMQAYRWIVDSRDEYTDERLEKLAEDVKVEDCQNIGMCSFTCPKGLDPQRSMNHLMKLIEEYKERKIASATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y429</accession>
    <name>A0A8S1Y429_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1490119</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000149">
        <property type="protein sequence ID" value="CAD8208067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y429"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000051">
        <property type="entry name" value="Protein phosphatase 2C-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="87"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8208067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="368" mass="42641" checksum="DF008DC81EF02889" modified="2022-10-12" version="1">MKQLNNKYKQQLQPLKLTNHITPQFKLNPYQNSYSPMTQRKPLFSLHEKTSNRSVKKQMNRSPLITQKNTLQIEKCGLDQNFEYILKFAVRTRQGMQIGNPNKQNQDSFIVFPNIADKSYMHFFFVCDGHGIYGHHISNFLKQQFPIYITKFKNQLENNPYATIYIIFNQVIKALNQSTIDQQYSGSTVVGLFMLHNKIYCPNLGDSRVVMLSRTNKWFLKNLSRDHKPDCQDEAERIINHGGRIDPYKDQNGQVCGPLRVWNNGNVPGLAMTRSIGDQIAKSVGVIEKPEIFNFVLEKMDRVILLGSDGVFEFLSQQDILDSVTPHVDRMDVESACNHLLEMAHISWVQRSNKVIDDITFILIFMQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-02-22" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y924</accession>
    <name>A0A8S1Y924_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1570001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000157">
        <property type="protein sequence ID" value="CAD8209893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="534"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8209893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1081" mass="128138" checksum="94D14FA66CD8EA66" modified="2022-10-12" version="1">MLSLVNTKISSLRGGGCGSFRMNPRTYENLQSDIQDIDNFITKFSSFVEIICAKADVAINQIESQEIMIAIQWFIFQEENIYKLNKNPQNIMKSYNLIVEGIRKLLKSCQIYIRTDQFKCLYILQITASLSKVIFSFHVMNSDRNMNFDLQKEFLDISDDLKQYIEIEKNDLVQIQMEVYLFLTKTSFEIVPNNSKERQDIVKGFVSGIFDSLIQMKPNAELLKFLFNVVHQMHNIYKIKNNRKQYEVYFQIDMLQWEIIDFFRNEKQLILNEIILQIQLIYDDLVKNSKNWINHFLWIQMIGKLLIFNPLLTKQRLNQLMNSNDFGVNSKQMWKEYQKKGLLIKMNHTNDQAVILLSQVQKNKLIELDRQVLEESFKGWEDFLIFKQYFIGELNEGTPFTFSSYLKCKLDLFRKDSQKNVQILTIEKMKMLLSFIIPTKLINLIKQNNENLGEVIKVQKQFRENDQVNKRLLQNTVDSSLKKIICNLYEYLQNTQKILKIVKLNQNNNYCQTEQLKQANKFEFNILFQLKYLMKLLEEILQQAIQEIKNKRKQFRKQDGINDEIIILQQQKELIQEQLKNLNLKDNQFSFVNKLQQQLLDLLITTLDQKSFLKKAERILRLIEDSKNQILRLEIMSFKKCKFIECFQSLFNHLNFEQDHMVEKIQKDVNESILSFKAILISQYELESIYEFVEIQQQISNMLSFQFVYKLRLNNLKLQFQLNALKESFQNIILAQLEEFQQLQKVIDLDEFLVNVINNYPKKKIFCCHDNFYKEIIAELTSMQITEQDYQNKLSKQKGIFEYLILVLSLEEQLIDLEKIDLELIDKEFGEFFKEESCSSSLTDRIMTIIENASIENSIKTVTNNQFNKSELNFEIEKYQHMWKQLKIVEKNYNCNNIENVKQLINHIEMVVELLKNSQDQNLEILKIPIRELLEELKNKKIEFQKIIQNDHQYKQNKNDEIFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQLKSVNLQNVSRVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YBP9</accession>
    <name>A0A8S1YBP9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1620075</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000162">
        <property type="protein sequence ID" value="CAD8211170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YBP9"/>
    <dbReference type="OrthoDB" id="283964at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8211170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="103" mass="12439" checksum="5B8257EFBF890743" modified="2022-10-12" version="1">MQKFRFENKPSKQFPLDNKVWKLQEHPCIIDEPQLTEESELGYQIEVIRIFNDSRCQQYTNSMILNNYTVHSVSQAFDNQIDSELYSENINNKWDQYQYKQVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YCJ7</accession>
    <name>A0A8S1YCJ7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1640005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000164">
        <property type="protein sequence ID" value="CAD8211490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YCJ7"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00882">
        <property type="entry name" value="Ras_like_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="1">
        <location>
            <begin position="7"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8211490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="466" mass="53701" checksum="BD63FBB03B6768EB" modified="2022-10-12" version="1">MIRAQRQIVLTGYIGSGKTSIFNLICKTKQPALFGGCNSQTRQPFLKEAAYGSGFRVLDTPGYGLISEKLIHAVGVLNALSEGPVNQIFLVVKWERIELMQVYLKYMVVKFLRFKHLITVAVTHWDMADKETIKQNEEQVRKMIASYKLNSVIFLSKLDSGENVCAQIDTILDKSQAEKIELTEAEFYSNFDLIELKNDIEFELQDQTEEVNRNFRKIAKAIRQFIKEFDDNNPSMHEIMHYLALETKKIAEGLISDFEIKNNDKFSELFEYHSNPSLAYLIHFELKKALMIDIDEIVKLTQYKMKNNQEHFFNFIKACPFCGLIWLKVYGSCTTTTCGNFPTTDDSKFSKYKLTQKYTFNITEKGVTYTLNQENLLQSNPTTSKTYVDPSLKEKQKKLGCGKPIIWKEIPALSPQQLKELIDPGLMDYFANEVPNEELKKILMKAQKSIKDKVDQIKIENTVVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YGK6</accession>
    <name>A0A8S1YGK6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1750027</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000175">
        <property type="protein sequence ID" value="CAD8213205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YGK6"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="261"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8213205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="336" mass="39834" checksum="61C0B7C82EBE2448" modified="2022-10-12" version="1">MGVEEDISELGMQLSPGKPIYINQILDLTQYKKVSEYIKFTTSLRRGISQEQVDGLLNQIGIIENKIEINILIIESLLMKGRIIYIHKNGLFPFLAPEIYQPIKVNGIIHLMEWIIISLLLPWREMPFILLQSYLKLELILTWQIKRTRVGNFRKFLEAIQFILLNANYQKVSKKLIVLLQKQYVKCGQQLKLTNDYLEVLRFKTEEQMVISQVQQQSVVCLQQSIIIEQKLEPQQVIQQDLSFQQVNFSSIYSLSLQLFLLYRLKLIILSFFLYIFSKTLILIIQQRTLSRMNSFTKIFLLIQEINSNKIYDETFRFKQERKNFFSFCMPVIWQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YIK3</accession>
    <name>A0A8S1YIK3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1760025</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000176">
        <property type="protein sequence ID" value="CAD8213281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YIK3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="108" mass="13472" checksum="FA164A91F1EA0D4E" modified="2022-10-12" version="1">MLESSVKMIFQCFNIKWILIQEYALQIKEIIFQPQYYNLFYKYTLNKNGEYYTKTKIFLMLMLIQMMNYFLPNITRRRAFFYLKIRMTCKYIMLKISRQEHGVQFKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YIX4</accession>
    <name>A0A8S1YIX4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Matrin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T1640016</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SF3A2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000164">
        <property type="protein sequence ID" value="CAD8211512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YIX4"/>
    <dbReference type="OrthoDB" id="10250970at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000690">
        <property type="entry name" value="Matrin/U1-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052092">
        <property type="entry name" value="SF3A2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031781">
        <property type="entry name" value="SF3A2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23205">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23205:SF0">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16835">
        <property type="entry name" value="SF3A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01050">
        <property type="entry name" value="CactinC_cactus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50171">
        <property type="entry name" value="ZF_MATRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Matrin-type" evidence="10">
        <location>
            <begin position="54"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8211512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="220" mass="25795" checksum="57CAD6DE59D932F6" modified="2022-10-12" version="1">MDMQNRPGGRTGSGPMASAADANVERRERLKQLAMEIIDLQKDPYFMINHLGTYECKLCLTLHTNEGSYLAHTQGKKHQQNLLRRKAREGKDLNAMMHMAKQNQPKPQKHKTVKIGRPGYKVTRTVENTSKVLYFELYYEDIQPGFIPKHRVMSAFEQKIEQPDKNYQYLIFAGEPYENISFKIPNQEIETQDGKFQPVWDKDKKIYSLRVQFREKKNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YKD0</accession>
    <name>A0A8S1YKD0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphodiesterase</fullName>
            <ecNumber evidence="1">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1640017</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="1">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000164">
        <property type="protein sequence ID" value="CAD8211514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YKD0"/>
    <dbReference type="OrthoDB" id="74705at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="2">
        <location>
            <begin position="628"/>
            <end position="975"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8211514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="975" mass="115000" checksum="D8C9E5F652251ADB" modified="2022-10-12" version="1">MLSNPHLSNYIRFFQIISDMEQECLITQKEKHQLKVNLTLKDMQLVNMIALNTDADDEEFKYSIINILRKQNQSPRQRQGQKIRCKSSGIEMSPIRLIRRNSVSKEAKITEEQLIQQIDDLNDSLVMILQSENKCILTPIVTEKLKSILEVIQKTTISSPSTTTTKILQAEESENCKIVDKFTQLYSDMKKHYTDYLNCHLLLMDNNLSYDNLVQSVKNFIKKLIDADQITYLHIRDDQSQQTYYSADDKYLITNDKQIYNETQLIQPNKVLLLDECNFYPMLSEQFKIKLYKKNYLIKLNNQDVFVCFHISDQTSIISEFLSLADEFDIFEDLNQLSSFLIETIKQAKIEVFTPLQLSHMIQGIGIAFIRSSKYLFIKKCIHILQSKYQVEIHINSGDGMSVISSPSFFYGIQKQVDFDFKDELNVSITINGMDLNKKTDLMIYKQLTQSFNKYLKFIRQCYDRTTFYKFFVKSQDTLIFEFDKLGRLIFLSRPIPLEIKKEFNINFDPKNTKVTYQQLFQNEELLKYIENFLQEHNILRDSDQQYQVFMKMEERNFKGFFIIFQQGWFKYDQKKFDLNDPSLQKELQKQIIQQETIRFIDKLEQNNPQILNSVVQMFKPRSTQRQTVHYRNSILQFEPAKLGVQHKCSLRLEDLDTLCQVPNIDNFNFNILIVEEIVQKQLVVVEILKYHNLIKEYDIPLDTLCQFLSEIEYKYNKKKNVFHNYDHGISVMQNVHAILLQLHKTNNATILSLFNQFALLLSGLCHDVAHTGRTNTFEINSLSNLAIRYHDRSVLEQHHAAKSLKLLCVPATNILQSFKGEEFRKFRRMFISNILYTDITEHFNLIKNFESKVQESNFGSQDEDIKLLSGMIIHTSDFTGGAKQFELSKQWSFKVNKEFEEQYELEGKLGYPQLPYMKDLHKLPVMAKQEAGFFKFIVRPLWQSMSKYLDNSLQQQVDYLDETIQKWEEIANSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RV16</accession>
    <name>A0A8S1RV16_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0010300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RV16"/>
    <dbReference type="OrthoDB" id="296926at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="211"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8131816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="383" mass="45501" checksum="C20C3864AAE09B24" modified="2022-10-12" version="1">MENASNFIRMNRDLRFNGLQQTVKGNIKVKDVKFLLAQKADTFPQLLVAFTKFGILEDETELFKIYDNIYVYSIGYLIEKLKNDVYNKKSNPNNNSIQLSTIIRSFKDRKSFNEIDHTFDQRQYNSVDTNYCQKKYKNGIDYVQEQIKKSMQKDQNQTKECNNNSNTHYYCQIQKTEESIKPYEEIRNSKSSISHQQQQGSRIYYNLNSPAKELQKQIQKYQDIIEQKEYQIKQLEQINQSSTLKMQIQCQQIENQDKYIIRLKQQELKYVKLLGTISEYLQKIIGQIQQKLERKQKQQLEKEKSSTDFLTRQIQSARADYKIKIKQQQLFISNSKLDQIFSAKLIDLQNQLTETILLNQSIDDLVQAAEFLELDSSFRFDFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RYX7</accession>
    <name>A0A8S1RYX7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0020013</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8132672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RYX7"/>
    <dbReference type="OrthoDB" id="283074at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8132672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="192" mass="23673" checksum="0594D4F50BEF6B29" modified="2022-10-12" version="1">MSIVTRFSSYFIKSRVINYSLQVDRIMTEMCKAGFQDPEEGFLERDPMTYYECRFYSHIARNWTPRLESFEVSQYELAKQKFIQFENLYQFILNLHRLTWEYRSIYLELTKEIATHNTWFRSEYTTLTYEYHLEEAINKYINLLDQLKEYPLWQERVKEEIGYYLHLIYNSTTHSSQSKELFAKFDKLYFFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S614</accession>
    <name>A0A8S1S614_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">STOP protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0040010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S614"/>
    <dbReference type="OrthoDB" id="297356at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="167"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8135013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="463" mass="53861" checksum="996C66A9DC3C0455" modified="2022-10-12" version="1">MISETILEKLKPSNSYIVKPTEKTLTKLTKSQNSLKRYDKDEKHSDDLVKFKETLLSINASQKSEINSSREPKYGKRVLLKSLKGGPLIPLPKNGTNRHETPPLISQSLPQLKLKTLGNLQQRQISSITEKNQTLSKSLLNNPQMIQETEQSLQSDNRSMLYKKEPQQVLQKKQQQENMNEQIVDQQEQESEMAQNKVKPIQQVQEEHQEEEQQSQNISEQEHIQCLVEQHLSEQEEFVKHLAGKCICVQCPCGRCKCKFQYKPFATNLCWNSNYRSSYKENPIKEQDLKVDLEQFKKFKSVESCEYKTTMGSDFKSYQIIPPEQKVSKTYKPSLGTTPMTSYKNFYMNYGDLHYEQFKQSHYKTVIPELPFNPNTTYKQSFKIQQLQDNAYTKPPNGKPFPTIDLFLGQSQNKQAYQPKQVEKAKAIDQHGEIMQVPAYQGQYTSVTHNDYVKKEILCEKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S8R2</accession>
    <name>A0A8S1S8R2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0050155</name>
        <name evidence="3" type="ORF">PPENT_87.1.T0180339</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S8R2"/>
    <dbReference type="OrthoDB" id="282470at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8136448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8148981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="96" mass="11440" checksum="65B17BD6C1B1C96C" modified="2022-10-12" version="1">MPVPNSSYPIPLRTVLKIAPVCMVLTTLGTLLPVCLYDILYHSHERIDKFFWRSSRFERFIRCRDMKLRTFWYEAMEWQPSGRESFISTRPQVADP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SBT3</accession>
    <name>A0A8S1SBT3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">COMM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0060196</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SBT3"/>
    <dbReference type="OrthoDB" id="77522at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037361">
        <property type="entry name" value="COMMD10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12333">
        <property type="entry name" value="COMM DOMAIN CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12333:SF0">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21672">
        <property type="entry name" value="COMM_HN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8137646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="218" mass="25267" checksum="C0394F2B92AF0681" modified="2022-10-12" version="1">MKVNKNLQDAILNLNSLSIPRTEIVIGRIFSKPGQLYKDLFTEDEIKKFQNNLNINEQQLANIVNAFNFVLMHVVAERNLSQVEEVLGQNGMSQDHIEVFRQQWIQYGNEYSIRIREKPIAVQDVLHSFNWKISLQVDESRLPQKNVANFMNVDGKIEDANDLYSFDARNPATTFVFETKPSGETNKVDKFSIKFQKAQVQELFEQLESIQESLDKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SIU1</accession>
    <name>A0A8S1SIU1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0080033</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SIU1"/>
    <dbReference type="OrthoDB" id="29755at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006818">
        <property type="entry name" value="ASF1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12040">
        <property type="entry name" value="ANTI-SILENCING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12040:SF0">
        <property type="entry name" value="HISTONE CHAPERONE ASF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04729">
        <property type="entry name" value="ASF1_hist_chap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8139307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="219" mass="25690" checksum="BB4BED8115F90822" modified="2022-10-12" version="1">MALINITNIVFDQDTALFNSPIQMQITFEVMRQLDEEIEWKLIYIGSPNSDRYDQVLEQFSMPPLQQGTMQFTLMSSGPNFELIPSKDDLFGASAIILSVKYRKQEFFRVGYYVYNTYLEPELIENDPPQVLIERVYRQINTSAPRVTRINIDWEGHMVQLYVNPQQNNQSFMFQQQPIFNDITNNLGYLSEPIPDKIQQQQYSGELNNNQYTDLQSIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1STH1</accession>
    <name>A0A8S1STH1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0100315</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1STH1"/>
    <dbReference type="OrthoDB" id="269518at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23833">
        <property type="entry name" value="UBCc_ApmR795-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00198">
        <property type="entry name" value="vWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000111">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="319"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="4">
        <location>
            <begin position="717"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="domain" description="UBC core" evidence="3">
        <location>
            <begin position="967"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="118"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8141804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1156" mass="133204" checksum="454520EEB5F5C0BA" modified="2022-10-12" version="1">MNKLVLNIQFSQQVKFKIAFDASNQKTFDQLKQICVQRLEAFYKISPDNIKNTLPSIFDPSQYHLTDQTNFILSDLDLVEDLCSNNELINLVYNQSLSTSILDPLKTSNLSIGSQLNTQSTQQQQQQQQQQQQTIPPQQQIQSPQSSQQVKNIVQPLYKPQQIDDGMMDEIGKNQLKRKFYLDTSQWNSIQQIQSDIEISIKFLQAEDQTIQYNFGCSLEDTLFNVSVKICNQLQLSQDQNHAILYNLSGLPLVNNMNQLHQKTVDDTISKLQSKVFYVIITKCSAGDKSFPKIDSEEGKDQIFILHQGPKVIKVDIETTTVLQLKQKLYHKLNIPTNVQTLNYGGKLLTDTLLLKDYNIQNNSNLNLTFKLNQLVGHADSYNYNFYMPWLNHYVKQSDDGIRQFRCNMLVLLAKEDQPKWTYTIRQYSNNPALTLACVCLFRKYRLNQNARIAFEEGFLRLFFDLIIQSSQVPATFDIGSIFEYTRQWIQFLHNEAAKFKEADIKPVEIYRNIDYSCQVTLSPIENPAFLSLNQNDDINAYQIVDYNIVKGKQETQEKHANGKLFSELQLIQDPNLTEQFKIVKFLEKEDLIWLPQVDLKDNRELQAIPAEWHKNEDLKINILKIYSSVELKQHPVAPVLTKNSQKQLVIYTSMNKDVAKPVNLFDPLSGMEQPENPDQIAYNQVLPNKSTSVNDDDDDDSSIQLVTQITIEPEEAIIVLIDISGSMDEEFYKSEDLTRLGVVKAFFNAFADRTMAYNLKNVISLAYFDDRYILKCGYTELFMQFKDLVNKAKPQGMTALYVALKNAVDSLLQFKKKYPNCILRIIALTDGEDNKGKYSPEFIAQTIVQNQIILDSFVVYDKCDGLKQITKAAGGQCFCPKTIQEGLKLFEYETILSAKIRKSQEPLTNAQITAIFQQNPQTIQFDVQPREFELPLELKKQSINPKQLVRRISSDSQQLNLIAPHNYLKRVMKELMTYEQKPHPFVKVFPCTHDVSFWKIIMAGPEQTPYSGGIYILYMKFPQEFPLQPPDLRFLTSIYHCNINSQGRICHSILGRNYTPDTKVLQIFEAVYGLLMTPEPDDPLDTTIASEYMQDLKLYQEKATKHTLKFAKRPMDEVLKEILGTVGEDLDLSDIELQEKINEINNWINNASKPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SY94</accession>
    <name>A0A8S1SY94_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0120221</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SY94"/>
    <dbReference type="OrthoDB" id="292517at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8143422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="425" mass="50054" checksum="A093F31079F31DC6" modified="2022-10-12" version="1">MYEQPEASPGFNRNQIEQQADSQREFTYQQYANPPQQSLTDEQKSGGNLNPYVYTSYNMPKINCYNHLGQTITNFCKSKECRLPLCPECVKEHVNEHSQFKTYPKLECLENILTQVHSDICQQANQITNALQNIKKSMAQAYSQKAFTVEKLKEAKKRILNIIEQYFDTLENELENKQKKNYENFKRDAEALIGALDARKGAYINFLDKLQQPECMYSLLPYLASPSYEDNQQYIQVANKFTTRFKEVQSEITFDQYKSVQLSSHLAEIVGVIHQDLTEYLDMHKLAPPEIVKSKALTLKSQNNQLQKKTQSPNQKQINQSKTHLIQENKDQEIIKHNPNQNQLEQTIRYQQINPQSIQIQQHQSPSTFNSLNQIYPQQFTQQQPQYYSNQPQYQAQYPYNQQRYQQQVPYQQYQNTINPMQKYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T3M7</accession>
    <name>A0A8S1T3M7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0180022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T3M7"/>
    <dbReference type="OrthoDB" id="1919336at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00084">
        <property type="entry name" value="HMG-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="1">
        <location>
            <begin position="33"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8148351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="124" mass="14887" checksum="3C9546410E1F0DBB" modified="2022-10-12" version="1">MKKKTKRGVSTHSLQSQKNQIPFQNYYFKTPVLSAYFLYLEEERWKAVRDQPECSMYQIVKQASTNWQKNLTNEQRNVYAEKAKQIKNESDAKEYLKMLEQTYTIKKKIQKEKVKQKSLPKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T5J7</accession>
    <name>A0A8S1T5J7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4378 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0150222</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="308200at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8146194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1150" mass="137865" checksum="E2CBEF40387701C1" modified="2022-10-12" version="1">MQDQKGKVYKKKKCFKSTKKLQEIPPISTQRTLKTTDQILSQRSPITGTTKNTIETKRSMSPEELKSFRKSNKIQKLESHQQSNIKIPLFTEKLQYNKKLSIQKFIKEKQQSNFIKEKLMQQKIVNEKQKKLFNLQKLQIKTNQIFENAIKLKQKIQMKKKLKRFKLYEIIKRFQEISKRYEIIQLNQIAKFQSQIVKHSQSQKPQTKNETQININEEEKIKELNLHQAAFLIQNKWKEFKKKKYSQTKLQQISLYWEPQQVLSSNFEDDFPFYQVGEKQVHISNIRSSIISEESSSIVNQLNQEFDNQNLFTQRIVKDKDTSSINKQMQQQIISIVQNQLKQQGNIADRKSENEVLKERSIXTRKKSVSNLLKNYKRFPPFLLKEHSRQPTKYYHREVPQLVLQKIQLKLNEACHLKNQNLSEKIPLFTEKLQYNKKLSIQKFIKEKQQSNFIKEKLMQQKIVNEKQKKLFNLQKLQIKTNQIFENAIKLKQKIQMKKKLKRFKLYEIIKRFQEISKRYEIIQLNQIAKFQSQIVKHSQSQKPQTKNETQININEEEKIKELNLHQAAFLIQNKWKEFKKKKYSQTKLQQISLYWEPQQVLSSNFEDDFPFYQVGEKQVHISNIRSSIISEESSSIVNQLNQEFDNQNLFTQRIVKDKDTSSINKQMQQQIISIVQNQLKQQGNIADRKSENEVLKERSINNHQKISNEDLTQTNLLQLQWLLQQEQNLILNYRKEALYLRYNIEQKNTTEKDQQLLDEWLNRELEDLQNTRKAFEIRQRREISALKKIQRDILIASAEPQFDLQDSLQIQQIQQYIDEQFNGLKVAKQNQKSQKNIQETVNFLTIEIINDMITDFSEEILNKKDDLYLIISQLIVDQQQPPCVPTSMSDIEQYIDNLFKYILDHHKQSLIKNMNTPFGFTPLERLRLIHGNQEDEVNNDNQEFQKTIDYPLDETIYIEYENQRKCMTENDDEIEWAILELQRIHNKAIFDACNEVLNHYRPYYYNSDIKYPWEQNRINLQQISNENLTQILEKVKLIVSKQSKVLCGFYTPEDFETVKQEKQDIIDEKHILMLMQQLSQVENPLLVDTNVQFDNLDFIRDEQLYKLLIEDIKEQEYKWDLIENNRVEYLMELSDQILEILIDEFSKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TBA8</accession>
    <name>A0A8S1TBA8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0180301</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TBA8"/>
    <dbReference type="OrthoDB" id="10249433at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF197">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_38, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="203"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8148907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="423" mass="49300" checksum="4ACD80EF600CD7B6" modified="2022-10-12" version="1">MDLDFIFFPAPKETLDIGYFDEGRILWIPKHSALYPNFQNYVKRKTKKNMVIFVKQSTQQVEEEQLSLIDDQQEFQKQPIPQFPNIHRVHSNKQNLVENFEFEEKEIGSPEFDIFDYEEDLQDKLSKLNQESVYNIGKTKISSIQQNQFTRAKKRETTSRSSPHFLQFNKKQDTIIQQKKTSKRDGNIIGYIPCLLIKYENSSNIVVYFHGNAEDISQSYTFLIHLRNISVLAVEYPGYGKYNQVQTSAEAIQNDADYVYNYLTKKIGYEEKSIMIFGRSIGSGPATYLASKHKPGCLVLMSPFTSLKDAVRDYVRFVGTWVQHLIRQRFNNLVNINDVTSPTFILHGKKDDMIPHSQAQKLKENCSAEICILHLAEDMDHISYKLHSDLIIPLMQFLRKINFYQIYFKSPKVPTNLYQNPIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TCF1</accession>
    <name>A0A8S1TCF1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0200100</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TCF1"/>
    <dbReference type="OrthoDB" id="29072at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001025">
        <property type="entry name" value="ATPase family, AAA domain-containing 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000022">
        <property type="entry name" value="Fidgetin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF17">
        <property type="entry name" value="FIDGETIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="196"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="113"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="126"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8150120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="451" mass="51673" checksum="115ACB520648215A" modified="2022-10-12" version="1">MITKQEARNQNFLNLSNQKQTEVFLKNQKNKQSLLKVHLDLKCINYTQMKLFITNRSEFIKLNIPANIRKQEIKVEQPPQPQQGQNQIQQPQISNQQQFGFVQANQLMPKKRFQPPTKIGEKSKQEQLTQKNDKQSNQTNFEDNIINKIESDIIEIMDRPTQWTDIVGLDHVRDQVVEIALWPLENPKLFEGIIAPGSGLLLFGPPGTGKTMIGKAIASEGKATFFSIKASTLTSKYVGEGEKTVRALFALAAQRQPSVIFFDEIDSLLCARSEKDNETSRQIKTEFMVQLEGATRGGCERIVFIGATNRPQELDDAIKRRFQKKIYIPLPNKEGRQSYFENLIIKEAKEGKRIEMNNQELKSLVDLTKGYSGADIRNLSREACMYAIRDAAKMYTIKNLKLDQIRATTIDDFKRALQIVKPTVNQNDLKDYLKWNQQFGSYNYDADSLDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TED2</accession>
    <name>A0A8S1TED2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0230017</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TED2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8152371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="16019" checksum="247F3757F9C44175" modified="2022-10-12" version="1">MEQALKTLQTKLPTKPIQLVFIKKNSLNRKYSPFQIQINQSSKYQQLTETQREQIAIKQSHMKRIQKGLSSKKQCTQNQQNKQLISMPQQLHQKFLCQKQKDLVQILIICLTILSLIVIIFEYHQQENNKKIQEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TFS6</accession>
    <name>A0A8S1TFS6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tubulin-tyrosine ligase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0200073</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TFS6"/>
    <dbReference type="OrthoDB" id="202825at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070740">
        <property type="term" value="F:tubulin-glutamic acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241:SF147">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE TTLL7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="477"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="560"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="385"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="477"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="488"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8150066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="746" mass="87760" checksum="7D9FC60875C980DA" modified="2022-10-12" version="1">MLKLNDENEIPFNGEFEDSEEESEEFSEEDSDDDELINKRKKKRKQLVNNNKRLIMNVSDTQYAAVKFVGKILNKFKLQYTPYLETQVWDFCWTDNAVLPETLARMQGHQRINHFPGMYCIARKNYLGKNLNKMAKQFPEEFDFYPKTWMLPSDISDLRQNIGKIKYFIVKPEASCQGRGIFLTKQVESIASEHCVVQRYLHKPLLIDGLKFDFRMYVLLAGCDPLRIYLFKEGLARFATQPYQQPNQMNAEEMCMHLTNYAINKDNPNFVFNTDEKQLDIGHKRSLSSVFKLLSQQTNIDELMAKIKELIIKTFCSVQPFLQSNYTQPDNYANNMCFEILGFDILIDNTYKPYLLEVNHTPSFTADTPLDALIKKNLIRDTITLMNINLKVKNELQQLQKEQMQKRVFGKKPKLSNEEKKSLKIQAQIEREQYESQHLGNFEKIYPCDKSYDEYLQHAQKIYEEWTGANIRRNVKKETATERDKLPQQRQLQHSITIQGSIDSNNKPQMDPAKPSIRKSLYKHVHSKVFSNINPQKSMTELPQQQQQIEYKLQDGNDNEIASEDQQNNSKQQEKIERSKRPPSFYVMKSVKQSSELQLVDLFNYKRISIPETSPQSQPIILQQQSLQISQQQQQQSPNFKSATQLARHFSIRKTNKSKLKPSESDQEAKKDLIQAVNQYSQQQQQQQQQSFFQSQQQHFQNQQQQQNGSFIKPKVFNLKLQPPHFKIPSLPILMQQQNYYKFRYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TI88</accession>
    <name>A0A8S1TI88_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0210023</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8150802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TI88"/>
    <dbReference type="OrthoDB" id="421226at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="221"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="295"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="333"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="413"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="3">
        <location>
            <begin position="516"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="757"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="758"/>
            <end position="774"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8150802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1076" mass="125760" checksum="0827EBA9FEFFB0E3" modified="2022-10-12" version="1">MSDSIDIVNQQSCFQEDNFQGPNLMMVSERILLGDGDVYQKSPDSNSFTVRDPSKIQDLSKELIDSVPMDMRKSYRGLKKSEYQSAYAIEEELEISGKPQFLKLIISKSLQNNFINNLWNRSYLRKLHQLSPFQVQALDDLQFENEIFQEEDDNRKEINIFEIIKKFFLQLDVFTPYSKLIFIWDLFQIFTYVLIFFWLPYKISFSLDSIGQLLNFKSKNQMIEIILLTILSFDVVVGLNLAFIHKGIIITERKRIIVNYFKQYAFVDLVSISTVTIQFFILNDGQQDHDIDNNLTLQIIFCVIFYVLRMTKINKILAQIQEFFNLNGSLNDLVGLLKLTMIILFIAHICACVWHGVAYYNDSYSWLDAYNLRDQGNASKYNKAIYWATMTMTTVGYGDITAKNNLELLINNLTMFIASIVFAYSVNSIGIFVSNMYKGTMEYSRSVTLINTFMSKNKIQFELQTRIRSYLEYIWQEEQNMNDDEVSQIICKLSSHLQDELQFQLRGNILRNCKVMVKIFSEKMIKCLLGQMEEQSFSPEERIITINQIDDGCLYVITKGEIELIFEGMNNLNERYKRNSLKYLFQGDFFGELSFFTGEPRKCTAISRGFTKVFKIKRENFLKVLSSFPNDYEKFCEVKHQLKQGDFSSLQISCYSCQSNSHLLDSCNYLHFCADKEAILKKELYPHYQYRSRLVSRGAKAFKHNWDQYKYIVQRAKDFQTDFYQQPTDNENDEQPSQIANDNYTYEDESKIEIDVPSTLNQRSNSRSLSRVSQKPNQVIIEEEDDSKLYTRKVVNPRGTLQTAGFGNASLRESKKFDLAKEYEIEQPISEESSSEEKDQIIIPSVQKTNQDRIQKQKITFIRKESSEDIVPRQASLRSRTFTHNKLQSEILKRSTTPDRLSSEIEISPNQNITKRMTGKKHSTTKTFTKQVQEATEGNQNMTSMMDNQTYLPIIPQIYSQFDKMQIFTHFFPFSNYDIVIKRYQKIQKYFGKKRQYPESSKYSFYFMTIKKGWKLRKLGQKLQGLKLVDTIKKPFGKTIQSGKIIKSKNLLTNLGNTGQVIPYLKDGQVLQDENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TIT9</accession>
    <name>A0A8S1TIT9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0240033</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TIT9"/>
    <dbReference type="OrthoDB" id="295865at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8153105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="754" mass="87128" checksum="95A1FED670001582" modified="2022-10-12" version="1">MSAQKSKQEYSKSKKVSITKEQNDSGFLIVSPMKELSPIKEKISEEQKCIEVVEFGWQRHKLGGEIRYLEQFYIENPNLQKSDIQRLDLDSCLDEQGKPVYRKTTQALMQNMALKIQSRISMAGDNKEQNVEWNKKSLNIKQKNNIIMIIIKTDFVLFLLVLTVVGVQISASPEAKVYNCANIEMIELIGGDPLPIDTSKERYVSFQGDVRFSVKTHKQTMDSLKEGKDYFGTNSIHYSSIQNGVYIFVVEKSQENIIQTYIKTNLKRQYKQAQIPKAFYLKTIIEGDKKYAIHQIVISDKQEMKRELFKEEKETMQSFTGLQFSKKCVIIVFATLETGTNTDFTADESFLQGIQGILDDINKGKKCDQFFCKPPSITNLAEALSDLPVLKITCQKKMCAYSQGGQTKVLFTKYPFLQTDKNFRSLEKAVNGMLLTTISPKNQKFVSITAQLAMKCNIQDFDFHSGGYSGAFQSIKIGDQKLYSALIIEPGYNGIKRKLRTQTPELYDLLEGIDKVKSIYYFMIITSKGPEELFNEIVSVIEKTKVSDQKKFECTPIPKEDDISFIQLNSKLNTLKPQHKQDENSEEYEYVYEYAYDEINDDEIGEYEYIYEYQYEEVPEELNQQEFYEEINDIQSDSIEFDQSTDDDSDSEDIDNQNIPQADLLEVQQNEMCFYAFSECDYQGASLKICGPNPSIPRELQNFLIQSIKMPDQMRITFYTNQQQKITIEGDQECLQRPFEIDQMRPQRQLLSHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TJ73</accession>
    <name>A0A8S1TJ73_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0210131</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="301881at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000129">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057352">
        <property type="entry name" value="TPR_TmcB/C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25474">
        <property type="entry name" value="TPR_TmcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="286"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="314"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1216"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1321"/>
            <end position="1343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1564"/>
            <end position="1587"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="2">
        <location>
            <begin position="646"/>
            <end position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8151019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1629" mass="190797" checksum="6E2ED237B27BA98D" modified="2022-10-12" version="1">MDLQSNFSKRVSDRNGFQLYVDRAKNLIFTVTSQLLHEDQQPLTTSCLAIIIQYFQLTYLMFNSKIQNIWKNQPVSDAISYFLSCFLLSPYTNSSNYSSLIILLYLCIGIFTLSTMLTFFIGLGLNKKKQENNWIKKGLKILINLFLSILYLPIIDVFISIIDCGFDENGVYVHNQFQDQECWVDTHILHGIVAIIFSILFYCYCVCFSMLYFESKYIPTDPNSKMSGRPLIVLFTYELIQTTFQKLVDGNDNQYFQIILLLGGSLIIFQKFHTELPYNQKIIQKFWSITVTLNVWGTLLLCCSMFLEKVIFSGIIYAFISGLPLLILTVFKMDKLNFELLLINHSKVSDVQEIINQTNYLIKLLHFIKTDSDAQIIIDGYLEIHRVTCVREDCYLKIKNQPNIRLTNSLLRDSTLTERETDLIFVLGQIYYNQIKRFPESVTLRIRYSFLLSDYMRQLQQAFNELTQAEQLSPSFDEEFIILRKKFIIQEQLDSFQNENFGKVDVASEISFQNNYRQLLQYIEQTTLSQMEFWSILQEDFPDLAKVYLIGQKINKLLNLIDQLWIKIQKASSNISKAMRLYGQFINDVLQDEDYGEQLLKKSELYQQQIQQRKKQIIQFIGGDEIGFEQQATVIVSTSIEKFALVITLNQSCCHLIGYTKQEIINRKINLFMPNLFQKFHDQYIERFLLTSDIKNINKDRFIFLKDKQNYILPCYIVLRILHTIDENVNLAAQFITVKAFKPSCYLIVDQEYIIDSISATAIQLLGIENKYITNKKIRLDLLFPEFIENLSIYMTKFGGKIKYEQENFVNQFQLLNQNIVEKEQEFICIVREIINITTNEVMGFYIKLELCEQGNQNSKELLLPQKQVQQNLQFKYIVNQKLYLGEFVDENNSKLSQSILWDQVDQSSLGSSNQINNETTHQESNRRRTIRLSDHDKPIINYAENIRVLRLFQNQIQEIDDKDNDISEDEEKESVFQVQQDLNPLNDQEQIENNIFRSRKNLESIITSNVTPRVIVKVKWISNIIILIILTLSFIDYFITINQTDEIFNTILLVKDGNFRNSELGIILSSIFNLQLLNRNVFNLTADQAKIYELEQRSNINDSINYVNILNQEIVSKASSLTQSNYEFEFGLKNVQMQINIDGQFEYYDLNQAIQQILSKSLSIRDKKLNLINQQDSDVNFVIRNWLNGLQLYLKESSNNLIDNLISKTMEKISIFVVLLAVSISTINIGLFLLIIALISVSRSQSEIVSLFLDIPDKTIRYLYHKSENFLTNLQIGEDDDLMSENDENIDKEENAQLQKTLKSKRTKKKYKNANKEYRYFIYIIIFILILIQGYFILTYFISGASIENIHTLSIEFNLTARTEQFYRFVEICQRSLFYSRNMSILEKDSYDSVVENQDELYSHNSYMQQLHSLNIDLLQPNYVDAFNSLYIEDPCKIVIEQDQSIDAQSCSLAMDSGFSQGLNVGVPRFFENARYLKTIYDQFYDNPKANFTLLARGFATFRNITKDNDNSTNFILNLNNFLHGKENREIQHHYFKNAFRYLTNQFIEGLNNELGNLKLQRLAIFILFEVLLFVIYFLLWLPLALKMAKDIWSTRAMILMIPLKIILRIRSIKDYIRNQIQEGDQDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TN55</accession>
    <name>A0A8S1TN55_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphodiesterase</fullName>
            <ecNumber evidence="5">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0230057</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="5">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TN55"/>
    <dbReference type="OrthoDB" id="189220at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="PDEase" evidence="7">
        <location>
            <begin position="352"/>
            <end position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8152449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="702" mass="81980" checksum="C28AE0A8DFFAA7A4" modified="2022-10-12" version="1">MNLEQFINSYLIYLLCYPNNIIFIQYQFILQSTIQIFVVFEKNNKLKRYSNFIQNRIELYKSQTHITFSRLITMTTHNYSSQFLISYQQLHFNMLASRSDSQLRSLENPDKQTMRGESSKRSDKLLEIPRKKLNMRLYTEQEQKIQDLINSKSITISIIALVGIYAILIFVIVALEELMDETQFNNVSIILSWIELGILIVFILEIAVGLYAWGVMKYYKDKWLILDTLIILLSLFFVIFELADQKTSNVVKVIQAVFRFLRIFLLIRKAQTFRRLSTISTISTPAEKIVRFLSELKEVIELESMKLDIDYCIDKIGNNQLYQMGKLDEDNAEAMGWLNQSQQGKSSVRKIVTGDQQQQQKKVLANFQKLNIPKRVLELLDKQHDDLYIDPFECDKLSGGEGLIYLMLFLFENYNLYDVFLVKPKVVRSYFEEVTKGYQDNPYHNRVHAQDVAQTCNFLLNRCKFIEIGQLDEQDIACVLIAGAIHDYGHPGLTNAFLINSKNELALTYNDQSVLEMFHPSQCFKIAWTNQKANIFENLPFQKYRRIRESIISMVLSTDMAHHASELAITNSRVAAPDFEPYGKDKQRLMDLVVHSSDVSNPTKNFEIYKQWTERVLTEFWQQGDKERELGLPISYLCNRYTTNMAEAQLGFIDYVVKPTFSCIQGFLPAFEPYFENLDKNKAKWQELVGYYKQQLASIQTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TNH2</accession>
    <name>A0A8S1TNH2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sulfite exporter TauE/SafE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0230273</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TNH2"/>
    <dbReference type="OrthoDB" id="301459at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031464">
        <property type="term" value="C:Cul4A-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14255">
        <property type="entry name" value="CEREBLON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14255:SF3">
        <property type="entry name" value="SULFITE EXPORTER TAUE_SAFE FAMILY PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="118"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="279"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="327"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="358"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="392"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="420"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8152852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="463" mass="51891" checksum="2542DC5DE256AA8B" modified="2022-10-12" version="1">MTEVCQPNQTGFFHTCGQFSQCELNSATLVYSCQHDSPFTPKAGIIISYILTPILIGIGILGGMGGGVLKGPLLEMILNYSQSEATHIAYCFMFGGTLLNTALLMFEKNPDDERRPIINYRIAIIFNLAVPFATNLGSSLASFLPQLYTLILQELFLFGVAPILWEKAKNAKKAELKVSEESQKENTQNLNDSNLNQKIELQAIEGMQEQKDYIISNSNLNESLYSKFKQESENILPLMPILCILGSFGLNQIFIQMRSTNPNKPSYVGIEDCTWENDFMIFILIITNVLYDYLILQFGTKQEKTFNQINFLPKERYFTPISRFFKIYAGGFLAGFVSGFLGMGAGFVMVPTLLYSGLIPRCASATSAFIYLMISLNNLITLLTNHYLNWQTIILFTGLAIIGGSVFAKIGYILLGKYKIGYFVILIVFALDIANIISQIYYGVVFGKRYGLDYLTQANKECQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TP58</accession>
    <name>A0A8S1TP58_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0240180</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TP58"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00060">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16495">
        <property type="entry name" value="RING_CH-C4HC3_MARCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000128">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46210">
        <property type="entry name" value="FHA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46210:SF1">
        <property type="entry name" value="FHA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-CH-type" evidence="9">
        <location>
            <begin position="159"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="6">
        <location>
            <begin position="282"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="7">
        <location>
            <begin position="431"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="8">
        <location>
            <begin position="492"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="610"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8153387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="650" mass="75841" checksum="BAB6595B9C65206B" modified="2022-10-12" version="1">MGSCASMKQKKIDGNVQIVSYKVCITAFIWNKESHNLFDFESHQACKKELELDFSATLSMVDNQIIALSENEYVKKQVQLLRVDCEQDYVTLSGMSQKSDQRVWAILCRRPEFESLNRGDIIKLGRMKLQLLDFNYDLDALQQQRDQTDINDEEIQSQDLDPDGCQCRICFQKNATVSNPLFSPCKCIGSMKYVHLHCLQVWIQQSIKVKNQQSSTQYIWKKMECEICKMPLKSTYTYQRQIFCIMQIQKPIVPYMIWKITSDDKSKEGIIQVMELQDRAEIKIGRIPDCDIKLKDISVSRSHALIKVIKQEDNQYKLILQDNNSKFGTLLYAQSDKLLKYNLPSLQKVLYQIGRVLVYIQFKDKGKSYNKQFTCYKHPDKIVVQTKINQNEQATIIPNVEKDKDNQMSFQTYNELHQEDIWDCIIDPIIKNNQQIGGLYLADIDCALSKELLQQLQIGALLSVIDEPKVITSSSIIHQVINIPDCTEQNIQEFFPKTNEFIERHRQHTNVMVHCFAGISRSASVVIAYIMQKFNWGFQRTLNSVIARRPQVKPNSGFVKQLIQYETQNRNRSSSQQHRILNTINTVIDKMPYQSDSKNNKYFQPSKLDLNQLEMEITKRQQDLQILRMKYQQLNDKIVNRNNVYVSAFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TPF0</accession>
    <name>A0A8S1TPF0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0240237</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TPF0"/>
    <dbReference type="OrthoDB" id="5800476at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000596">
        <property type="entry name" value="CK1 family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="14"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8153467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="374" mass="43989" checksum="7E915A8C38B87C01" modified="2022-10-12" version="1">MLQFDQEKPIGNLYKITTKLGSGSFGEVFKGINIETNEIVAIKWESQEAQMPQIKHEFSILSAIQGNGIPKVYWFGQWEDKTVMVMELLGYNLEQLFNLVNRKFELKTMLMLIDQMLDIISLLHSKGYLYRDIKPENFLMGMNKTYNTIYIIDFGLSKKYKDKKTGEHIPYKENKGLIGTARYVSVNTHLGIEQSRRDDMESLGYLWLYFLKGSLPWQGLDVNNREEKYELIKTIKQTISIEDLCNGLPNEFLKYFQHCRSLKFDEEPDYKRFKMLFRDLFFKLDFIWDYLFNWIEQLQQEQIQSIIHKKNSKNSLSSLKGGSLNLKTDEEDEIQEKIEFKIGGDSSTNLKNVKPIINIDKFISKPNLFESIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TSE9</accession>
    <name>A0A8S1TSE9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0270221</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TSE9"/>
    <dbReference type="OrthoDB" id="293961at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="335"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8155645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="457" mass="54196" checksum="20DD302E90F95A22" modified="2022-10-12" version="1">MTQNLKFHKLNNALPNIQISQHTKIQQDYQDGCSTDRVINLVNKVKAKLKLVNNTPDARFNRQLQQFRILDTFVDTKTKLKKMNILNNRDLIESVKNYLIRRKIARQLGQEEADLEKIYEQYEVKFETEFDKAFLDSDCFLDLGLVKAPINKTKVLSQEKLQDLGKRAISQFQTSSEQMSSRDQVTKSQYDQNLFYYYVEDHIEQLKKEKEETENFIQKLGKTTKSTTNSGRGDEKRSTLIQQQKITQIDYDKELLKIVLQYGIDLNSTEYLEEEIYSAFKQKANDFIKSKINEAKVRHNHNIEEDQLKNKYQIRPQKKRISSMFEQQFFNFSKNYPPSKSRYQPESKEHTLTSGDRKSQMLQERMDNKKEKTLLLSQFNLKSKRIINMCNKDEQKLLYNEFIKDIQLMNLYLDEAITRTNFNRKLSQLGFTKEDQFLIKKQIVLPGSSVRNHYDNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TSU4</accession>
    <name>A0A8S1TSU4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0270291</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TSU4"/>
    <dbReference type="OrthoDB" id="286133at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47823:SF9">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_10, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47823">
        <property type="entry name" value="ION_TRANS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8155785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="284" mass="33265" checksum="7A8FD50B66697AA9" modified="2022-10-12" version="1">MRLHRNHQGAIQMLKTKIESYEHRHKQQILCQLFPDTPIKNQKPTHVYYIIEGEVRLEETQIERRKHQNEMFASTCMAKSRTLLIISAGSLVGEIEALHEKPYQTTAKSNVHYSKILLIPETIYIDLKKQDELYQSTETQHLKDQLILKKINQLKNRLELQEVSPERDLSPQTKISREDFIVQQNKRIQKKRNFEFINLTPEAPLFSVKKIKRVAAPDDKPENRARSISAPTKAKMLSTIKSINSIDKISKPEEITRSRANIINFGEKCRPASRIFWQQIFQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TTE9</accession>
    <name>A0A8S1TTE9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphatidate cytidylyltransferase, mitochondrial</fullName>
            <ecNumber evidence="6">2.7.7.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">CDP-diacylglycerol synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PPENT_87.1.T0260157</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TAM41 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.41" evidence="6"/>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8154817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TTE9"/>
    <dbReference type="OrthoDB" id="288202at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004605">
        <property type="term" value="F:phosphatidate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016024">
        <property type="term" value="P:CDP-diacylglycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015222">
        <property type="entry name" value="Tam41"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13619">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13619:SF0">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09139">
        <property type="entry name" value="Tam41_Mmp37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="16" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="17" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00029893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAD8154817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="319" mass="36884" checksum="E0495649B80F3124" modified="2022-10-12" version="1">MQQIPMTLLKQFIQTLPQSDLSFAYGSAVQPQFNYDYTKNKPMIDLIIAVDNVEEWHQQNIQINSAHYSGLSYYLGSRFIQRMNVEIFPIHFSPFVQYQDLKLKYGVVSTKELIKDLESWKWLSIAGRMQKPIVTIQEFENLRYQRACISNLQSAVAIAILMDFKSSMSLQELLYSVISLSYYGDIRFTLGGENQNKVFNILKGNYKNITELYLSNLGDLNNVIKIQKDGEILIDNSLKSLEFLCSLIPGQLLSIVLGEKDNMGTLKMMSQERINDLIKESVKRKNQFSSRRMLFYNLAMISPLLSVKYGIQKLSKRFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TTH6</accession>
    <name>A0A8S1TTH6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0260181</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8154862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TTH6"/>
    <dbReference type="OrthoDB" id="300500at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="100"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="592"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="47"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="190"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="609"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="620"/>
            <end position="642"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8154862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="642" mass="73587" checksum="C48A1EDD3B8F44D0" modified="2022-10-12" version="1">MGTCVQNGQQHQFEFNTIDQIRLYENRHVYMDPEQELEWKRQNNGEESMLKRQLEAEEQQKQQLLENQKIHQQMLNLYDSQNESSNLHLDLELRSLNLQPSDKIQQRSPPLFDAENKQDRSFTQGQSSKDKNKNQIQQPETKKQLIDYLSKGEKDVLMQPLPSLTNLQGNIELVYEAFDSAEITPLATPKQEAESECEELEEEPLQLEQEIVQVEKQENPEPVQQSPQIEQPKSQVILDNPLFQQHQEDTISLEDDNPEEVQPNPPPQERQIILQSSRGEQMIMSFAPNQQQLFIKSQQQNVPENIVEVLNHKPSLEKAVPSNTDILKIENEHVVALSAQLEPININVDMNNCEMLGPYIILDTGDVYVGTWQMGKRHGFGKQIFSNGAFYEGQWLNDFLQGYGRYIFQNGDYYAGEFVHGEREGVGVLVYQDGSSYEGKWLKNQKSGEGREQTGDGNVYVGTFKAGKKEGKGRLEYVDGSIFEGMFKEGQICGKGIQTWPDGRRYEGEWKDGKMHGQGEFIWGEGKSYKGEYVNNVRQGYGEYSWPDGRTYKGGWKNGIMHGKGLMIWPDQRLQKGIWINGQKQFTKEELAQLSKAKKSNTADTSVNKTKDSGAETKEKKPKKSKSKSKSKEKKSKSKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TVI5</accession>
    <name>A0A8S1TVI5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0280210</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8156230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TVI5"/>
    <dbReference type="OrthoDB" id="310081at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="5"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="415"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8156230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="823" mass="99636" checksum="3FA4B32F8C428C51" modified="2022-10-12" version="1">MKNQSEKSFELSKDQFYQTFKPFEYKNKKNSPFTISYLSKKQSESYWIKKRKIQGIYKDFFIQEIRKEIEIQSYFQNRSQKIMKIKNFTIFESKNKSIVIIAYNNDEYCVPILEYFQSIERGQSRKIQLSKQLLEIHEELEQNKIYHQNIKPNNIFYFSGEIIITDFGSNRTFQQNYISQFSKISEKQWQDEYYYYNPKIIVDIIQNQTDYDMRIEELNKFQEIMKRQNKFEKVLNNPQNSMNLDAWAIGVIIIQIFFPQKIEKNNIEKFYALNTKELENKINQITQFDNELGQGLKDLFFPEQQQQQQQQKQKQKQKLSFQQQISQSNNNLNINRSLIISQIIPQESKLKSIKIQVGFQDEQRKKVVAIYETCQVMDQFVIQIQDKQNPKLEIFQKEKNISEKSRKILDFHILLQLQLSEAQSYLNDLKNLKKEFEDERTELLRSEKRSDTNKVQLESLVEVIQKIEETIFQVENRIQALKYFYNYQMETPEENIIYYYNLKEINMVQWKKQFQIKCEQTENNTNYQQQQYFSQKKIKDLIKLRQKELKIDAHLCVNMNQKEQEIVSIYYLEKCFNVQKELLEKKQSQKEQLDVEQFPFNLSLKTRRKVDKFINTKQFEFLLDYTEFNSLSAKLRNQYLKTLFICLDDRIKRNVQSEVNQLLSAINRIYPLLNSKFKQKNGKNTVRVKLQPEMLPQELCKQIKQFFQQKYSFKINYDFQYLELLKKFDDIKTQKDYIGFLVNGKPHGQGFKRINENIMQFGIYRYGQLIWGREIVKVDNYINYYKGQFKDNKKFGEGVFKRYLFSEIEGNLFFQKPNQPQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TVQ3</accession>
    <name>A0A8S1TVQ3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0280092</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8156024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TVQ3"/>
    <dbReference type="OrthoDB" id="308926at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="120"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8156024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="236" mass="27395" checksum="1F05BDBBF5805646" modified="2022-10-12" version="1">MSLNTSMPYYRNSSREVKAIREIVQAEAIKSARLLSRDKVKVKQNTSIGDLNRSKGKANNSFYDNRNNSQIENSYNLSGYLANAFQERKENIQRIQMQDITNKNKFDENKQREIKYLMRIAALEKESSKLTELATKLKKENQLLRQQNNQNNQEMIIYQLQEALNVSKNENLALKKQITQLQNSNTTCFNNSVYRIQKHQTQNTNYTVNTTNNLSQDNIKFKELIPSYLVALSLAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U191</accession>
    <name>A0A8S1U191_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0310274</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U191"/>
    <dbReference type="OrthoDB" id="284062at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8158308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="133" mass="15673" checksum="BBCA6C9CBAA964D2" modified="2022-10-12" version="1">MISVQPEGSKPMKFQGGRIHLRPIDSIGRDFDLGNIETKGRRHFSFDPTPVMQYKPQLRVGFPKNVTNPEILFTHKSQGGPDKLTYMPPIKKHKEFISTMRKTVINKEYEEQQTENKRMIEELDVWEKKFRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U2A3</accession>
    <name>A0A8S1U2A3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0320049</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8158604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U2A3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8158604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="48" mass="5690" checksum="4EFB925DA3B91B27" modified="2022-10-12" version="1">MAKVLVKYEVWEGISFYLVYHHQLPYLLTVNCVRNLQFVNSNQALLYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U2E8</accession>
    <name>A0A8S1U2E8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0320118</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8158718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U2E8"/>
    <dbReference type="OrthoDB" id="310807at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039273">
        <property type="entry name" value="TEPSIN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21514">
        <property type="entry name" value="AP-4 COMPLEX ACCESSORY SUBUNIT TEPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21514:SF0">
        <property type="entry name" value="AP-4 COMPLEX ACCESSORY SUBUNIT TEPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="461"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="263"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="65"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="461"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="473"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8158718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="483" mass="56239" checksum="33A9280A0821CDDE" modified="2022-10-12" version="1">MSSILNKFKKQTQSETPSAIKLENTSFGQQTGTYQPPVIPAIQLPQSNQPRQKGRPGGGWDDEPQSQPEQISISNNKATNSINLLNNTPPKQNEQQNNIDVLLPPQPVIGNIQLSRITDYKKGQLLTYEELELGGFDYEFHLVDEVIKLGGIKLKQSDNVLKEFTRAISTIQEQLIGSILLNKLHSEDNWKVQIRCIYAIQYVCQQYQNYREFFQMNQQYLRVETDQQLLSGAIDQTLAEVNGQQVQKQKEFQLEFREPPKPQANLQNQKQNLIDILDTSQNDTQQQKQQQQKLNLKQLKIKQPAVQTQQQQQPLVQQQQIQQQQLQGQQKNTQQINLLDAFSDLSLENYNQQQFQPIQQQQINQQQQQQQQNGISFDDLLNSQTTNQQQTQPKKNAFGFIKKDQQQQQQTFQQPINLLNSPPQQYQQQPQIYNQVPQLQPQYLYNQSQSNQNQQIYQQPQQQFNNQQQSQNNKKDLIDFLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U6N0</accession>
    <name>A0A8S1U6N0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0310262</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U6N0"/>
    <dbReference type="OrthoDB" id="297694at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8158286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="435" mass="51772" checksum="D632787DD024ADE9" modified="2022-10-12" version="1">MLVLKFNDIITNQVQQQQQQYQYFKIILSRYSNQHFQIKKAYTKFSGRWSKGSDLERQIDYVQENLDVNLVAKLVNAFNGIEIIINTPQELYEMYQILHFFQIQEFQSQIEKFLIQDKSNILIGYQLSELYEIESLSNFYFEYFKEYGFLGIFNKKLDTEKARKLIYKNKNKLVPYHYLYLQANLFKKLLILHNNLAIIKFDKNKFLDQFQIALLISEYCLTNGYDRIKLQEIFSDAVIKEQVTNEQKQIILIEFEKQYKKNQMRTVSSISQSEEDDSSDTTIEAKHKNSEITISQIPIIEPIEIFRGKELDIKLLIGLKNLQFMFKTKYTSFGFYVSKKIELNIQIQDEQLQLVNQITKIQLNTSLRINWIRFDENGLNVNNRLIIVRSKGICRYEEDVNEPQGQIRLTGCRNFIIEEVQVWNTCFFALSWNQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U7D6</accession>
    <name>A0A8S1U7D6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0330034</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="321029at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8159249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="109" mass="12731" checksum="1311713A62CCDFB7" modified="2022-10-12" version="1">MALQKIPLNLISQYTINYRSLLQKQLLVKYSENLRKLXLYANLQIGGGSYDDNGDEIKIGKWIEESREFNNREQIIYIGEYLNGKKVGTWIEKTTSNRGITQEEIIYDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U7I2</accession>
    <name>A0A8S1U7I2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0340103</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8160002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U7I2"/>
    <dbReference type="OrthoDB" id="293562at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8160002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="374" mass="43818" checksum="92CF400283510AA1" modified="2022-10-12" version="1">MSQSDENDQFQQILNSLEISKRKMSISVHQSKLLEESKKQQSKQFTMELPKNIEASQSQKERIITNHLLKDLAPLDKQNEENLKTPKVPPTNKIKTIKKGILLLGQKFHKDLGFYLKTMEPLDFQELQQSNELLIQNALCAGKYVNDFGLFCVLKEALNKKNIKTIIQQDCILDEDGNFAGIPQNTKNLRKTLGLQELHYCSGKISLLKFHINSRFNLSNYLFWSKELERLEIIIKDNLMNQDQQILMMKMEETYLEFTIPEFDDNQSPQFMSKLRQQNLDVQVLPLITHFTFRLSDLNPWMNKEINKEWKLFSVQCDQQQCIGYIDMNQPTIFNEIPRPKTLAIKLYGEIHQYQLVLQMSEKQVMGILLRDKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U8N7</accession>
    <name>A0A8S1U8N7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cyclin-dependent kinase 2 homolog</fullName>
            <ecNumber evidence="4">2.7.11.22</ecNumber>
            <ecNumber evidence="3">2.7.11.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Cell division control protein 2 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">cdc2-related kinase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">PPENT_87.1.T0360061</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>[DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10216"/>
            <dbReference type="Rhea" id="RHEA-COMP:11321"/>
            <dbReference type="Rhea" id="RHEA-COMP:11322"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.23"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">May form a complex composed of at least the catalytic subunit CRK2 and a cyclin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="4"/>
    <dbReference type="EC" id="2.7.11.23" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U8N7"/>
    <dbReference type="OrthoDB" id="541276at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000554">
        <property type="entry name" value="CMGC/CDK/CDK7 protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001235">
        <property type="entry name" value="Kinase, CMGC CDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF334">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 19 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="20" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="7 19 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="20" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="20" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="7"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="CAD8161220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="306" mass="35105" checksum="252E1A1C2554C177" modified="2022-10-12" version="1">MSQISKYQLQGKVGSGVYGDVYKAINTQTGKIVAIKKIKKQQQDQGVSAVAVRETSILQTVQCENIVKLIEIEYVQGCIRLVMEFYDVDLDTYLKSNRLEMNKIQEILYGILKGMNECHRKKCMHRDLKPQNILISKDGKVKIGDFGMARSFQQIPGSYTYEVITLWYRPPELLLQTSCYTTAIDVWSIGCIFAQMVLRAPLFAGDSEIQQMKLICENISLNQDNAQVLSNSVFQNQLEQKLDQQFKNTSMTQDGLDLLKKLLQLNPTNRISCHEALKHPFFNNIQEPDVINQNMDLLSEYFRVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UHF3</accession>
    <name>A0A8S1UHF3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0410115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UHF3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035715093" evidence="1">
        <location>
            <begin position="23"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8164601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="86" mass="9880" checksum="AC18780E1B362F1F" modified="2022-10-12" version="1">MVLIQILKIKIFLLIQIHLLHDFKCSQTNKKYIDSNYSRVGSMKSSDIDNISSNLSEQQIASFSKVSSNLLKISFILSIQRALTRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UNG3</accession>
    <name>A0A8S1UNG3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0430210</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8165954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UNG3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="55"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8165954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="281" mass="32608" checksum="D90EF42976B12AA0" modified="2022-10-12" version="1">MKVFNNPHFHSKSSSSKPSSLQSSRVGGSKIIGSPQGQEQDLNEMLQSLNPTQIIAQEEESKFDLYQEMQQLKTKVLGLLKKYQVMLQNENAKQVKSKIKSDVQEIQIEFEDIIAQYQIDQSQLEIENNIPTVEKEKELNTIFNLVEEFLERFSHHKATLNYFSILQQQKMSRNGLGVFSVDHNDRQSMYDFQESLPDLTDSNNSENTLEVQQAKIALSNSQRLRSQIRRRFFDIALKVKEGLPLYHPGRQILVSILFEDAEEEGIDEIEWESWIVKQLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UQH5</accession>
    <name>A0A8S1UQH5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribulokinase/uridine kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0460189</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8167021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UQH5"/>
    <dbReference type="OrthoDB" id="347435at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="1">
        <location>
            <begin position="18"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8167021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="251" mass="29628" checksum="BBADE7E51381DF90" modified="2022-10-12" version="1">MEQLQVLIQEKYKGSPLLIGLQGMQGVGKTTLGIQMKKLLSQLNIQFDSISIDDFYLSYIDRENLDKRKYKYRGPPGTHDYQMIIKTLSQFKEGKSIEVPIFDKSLHNGQGDRVGQKTIKCQVLLFEGWFVGYKSKCLYEFKQKEKLLQLDGKDYDQDLEVYINEQLKLYEPIWEQFDILIQLKPNPFELSLKWRLEAEKEMRIKTGNGMSDEQIVDFVTYFWNCLHPLIYNDLICDLVIEVNKQRQYKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UT75</accession>
    <name>A0A8S1UT75_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0490111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="296318at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="422"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8168448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1593" mass="186457" checksum="37C764F1911C7EEC" modified="2022-10-12" version="1">MSHFARLVELLNESAKNQQNSELNQLVQILIQFSFPQHTYQMICNSFSLLIAKTGNEQIKQVVEALLQYINKKGSQKSIALTLLVTLIQLTKHEIQSFSYTLTQDISTALMKLLKNSDYRLLLCNSLKFLIESQRNEILKIALKFTLNEKHEILQQAGMQTLIHLMENEVISEQMYLIAYKSDDSSLFYKYFARYLFQQQNIVALTKKDKLKQQVQLQPREQAQKIFQLFDENLSKFPSNIVGNIFLAYKKYLTYEFKKLGLSIIRGSFEISCSYLGSMALDDPYLHTVYKKTVNLCLFMVQQVQLNERKDILEYFFDKISKAKSQKWNVYIYKLLLKGMNFLFDTLGSNVFDDKRTKPSEFIDILTPIFKINDILQHPMSIDKPICISVSRNLFNTFESLFQENKKWQQSILSALLNYAKITLAELNVKETNQQINQLRVQCALLSKLFSQLDLQLHSISSELINHLWETAKSFLDKSSQLRRIGWIIIAELLMVPYFGTTNKVKEIGILIEKNLTSKDEIKDEQQLQSYCSVGLVIISILNSSSLSKQIKDTTRKFLAQQTILMFSKLQQSKFDKQSPFVISSKYIFYQAIRQIQPKYYSNELNSLVQFCLEDIINGNSIWNQVNVHKNNHYVDELLGIPRSEFQLVLAASKFLGHIISSQSFTLKNKINFIRYVNGTLQTQVINIKEVQQKQSKQQCYLFCLYQIALKIYQANSIVSQEFNEQIFQVINLCQCVDNQIQQLCAYLYCSMLQIKDVDQVEQLNQLLSQKSQANYTYLVLEILKKKLTGVQLQVQNLLMKVLSQMTTDVAKVIQLFQQIDPSLLKNVWVFLVFAISQSDKPIQICQFKILSGIILNYIRNGGIKDNYFNIIYQECRCRLQSKQFQILQLDFALEQDTEQTVTKAINLLLKKPSYKIAVFIDQLTDKYNMPSLVSPILFVLNNLFSNQRVHSTSEEMELRNLMKNIFIKVYNQPIAELLQLLQPYILDEVIPEQQERIPIHIKTRIFLLKRLQKLFKQCDTSNQVFDFLFKITTSIYEEAKEISFDMFQHFFYKEKDLLSFAAQIDSILCDTIRRENQNPPSVNLTNMKLISKFAKIVKDEFLATKLIQLLSKPLLNECRKKLVPSQFFSEKSQLNILIQRIVSLGSIYRKIPNYFDQTLIEQLKCYVEELLEDIFVILCTPRSHIREYQSYNFIYNGKKYSYDQGFLHQSLPKLLRISNLDKPIHFTLIGYIITQYGAFEQNENMFKKTIEGLTKERILQNRMYRIKGIQLLTEVINKNPQNQLLINGIQSLLPIIAAEAPYSLVICQQLYQSLIHHQIDFQLILEPLIQIYQLTVDQNQKLNCLASLLKISQQNHQALDQINIIFWDFLLTQEYEFMRCTQLIQFYKDKIYSIKYSGYIQTILQTISNAQKMRQEDYYLLLNLDILINNHQDNQRYYQIIDIHFTNLIQIALALIKQIKQNVQPILNSLLPAIYRKIQIKEIDSYILIGKFLLLSYLLSNQKLLLFQSTFRIMYLFTDESLDSSIVEASKQNLKIILINTQMNQVNLPDDLSDKVQLIQEDIQREMQEKQQREAKQKQPQQSGQIVLKMFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UWU4</accession>
    <name>A0A8S1UWU4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PHR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0500049</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8168904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UWU4"/>
    <dbReference type="OrthoDB" id="284179at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012983">
        <property type="entry name" value="PHR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08005">
        <property type="entry name" value="PHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PHR" evidence="2">
        <location>
            <begin position="218"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="84"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8168904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="370" mass="43470" checksum="D22CFA14597BD23A" modified="2022-10-12" version="1">MDAIYCQDQDHSGNPLNLFCLDKECQNKGLICSFCMLKYHQQHVKNVFPLKTIVSNLNQANNPKNQQELHNKGNQIFEQHHKIKQTFRQNLIEIKEMLNKLEKEIEKQIKVLDLEADLFVGDAFEKSITLLNMQKLNKEQIEQAIEKIEPLLKICDGQIWILRDTEINKSAQKLIKTAEVIERESKYWLEKCLVDLNKLYETCQNPIKKQVKILTSQNRYQLTYEQWNLKETDAICFRSKKKYDIWLSGVGLYEITTWPEQPLSFTVQIFEGANVNSKNVIYKDIFTIQPDMDFCNHQGKIYFNKAIKIEFDKPYTIALKPNQSCLSYYGANGKFETDEFEFSQPSFTQEFSDNSTTINQGVIPLFYYEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UZ80</accession>
    <name>A0A8S1UZ80_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0510136</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000051">
        <property type="protein sequence ID" value="CAD8169567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UZ80"/>
    <dbReference type="OrthoDB" id="304012at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8169567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="849" mass="101740" checksum="76AF31B064C9F369" modified="2022-10-12" version="1">MEQILVLIQQLFDQNESIRTQSEIELNRIFKDVGNIKQGFEQLLLPYSNQNLKLQYFIFLKNFIMKNYEYSPNYIQKEIQSIILEFINKFDSFFIAFYCDLLNFIIVKSLPSLDIIQSIINQPTYDSYHIKIITEMMNNPYIKVKVKHTEYQTITKRMIVYYNLIDIQKLNLLVDLYTSLIQSIYKFDETIKSTINHQFCEILTYHCLEYNLLKKFAKVINKLNKNGLPPQEIQTMLFDILMSTYMQNENQRITDKYLAYKILKVFKQLLSDRKIEDWKQESIIFFTMINLIMLDYDQYENQFNNIGDDSKQDYLLIKIRRFGAHFIHHMTGFISEDIMIKQLFRLKDLINDDYQHEEFNCIKLEAHYFVICENISSIPMNLFDEKFFQEIITIIQTSNNIYIPLKMQILNLLRKLLQKNVIQEKQINFFINTLQQQLNPNLIQYPMYICKIVNNLIYLHSLYPSKNLITHQTYTDLKDIFLVQQKLIHKENILMTLCEIVQLEQGDYSVISLIENEWKIGLQEQHQYKYRQRMILNVFEFIIEIWSLNLLNIIFIGIIRIVKDCLIQNTHFTKGLSVLCLLLKKYTAILPIKSDLILADFQQIQTFFLVSLTDEPDQFNQIIQLYYYILLHDIADYNLTFQFISKHTKQLYKNQQLTLLCLLFNNFKQGQYQEQIIQYYTNILQEVIYDEGRTYSELICGLSILDLNQYDVKQLLIEIQKKLPINNKIINIMIIKATNCIKLNIPNYINYLINFFNCMLYKQHLMYDIEQLTIENDDTQNLKQYISKMKSYLIPNNFSLELQVQRIFETKFFTKLTLCQEIAIKLKIDISKLQIQTEEQLAMENIHIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V148</accession>
    <name>A0A8S1V148_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SF-assemblin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0520218</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000052">
        <property type="protein sequence ID" value="CAD8170227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V148"/>
    <dbReference type="OrthoDB" id="307099at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038835">
        <property type="entry name" value="Giardin_beta-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37027:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_148, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37027">
        <property type="entry name" value="KDE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="44"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8170227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="248" mass="29138" checksum="4C5C2DEED1660F0D" modified="2022-10-12" version="1">MNPNTPFNERFFKIQEKLNSIPLHHDTSKAHRIDTINSRVTAVEERIQDTITSYNRKLHTLKDEIVRLQKQIEEENNVFETQFEQRVREIAAFESRITTKLEQEIALRRDGNLKLQGYLDEKVVYLKSDIQTESKIRQEQIENITTSLENDLPKLYDMVKTEGQDREDSDNGTLRRSGDEIRRLNEGLTNQKKLREESETAIFEMLKDLVSRVKSEIEEEKKLREESQESLLGLLEDAANKIYRAAKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V3E2</accession>
    <name>A0A8S1V3E2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0540247</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8171344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V3E2"/>
    <dbReference type="OrthoDB" id="46564at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF132">
        <property type="entry name" value="PROTEIN-LYSINE METHYLTRANSFERASE C42C1.13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8171344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="220" mass="25785" checksum="D96B72DDD25B3301" modified="2022-10-12" version="1">MYSEQNKRVIKLDEDEENTPENLITIYEKMNLETAGKIWECSLILGRYLIKQAYFNKIELANKNVLELGCGTGILSIILGKQGCNVLATDLPQIETLCEQNISKNNMTNQVKFKILDWNQSKHKTDCLIDKKHIDIIVASDPIYNQKTFESFFAQLKILYEVLTNKPILYLAHKYRHDEFDKNLEDQIEQTELWFKKVDSEHLDDLWKSQQCVVYKIGKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V5D8</accession>
    <name>A0A8S1V5D8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0580069</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000058">
        <property type="protein sequence ID" value="CAD8172818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V5D8"/>
    <dbReference type="OrthoDB" id="311052at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="123"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="17"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8172818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="226" mass="26023" checksum="B72EC84DEEDB65AD" modified="2022-10-12" version="1">MDNKKKKNKNKAKQDKQSQQQQPQNQIVPKNQQHQHDHNCHDHHGNDEHHHDHAHCNHGLPSDTSNLDPTSLLSGLNKGEGLLGSLSMIPKLTEMLNQLSNFGGQAFKSDDPLPDFTKFDQTKQTLEVAIEEFQQTIESVQKKDFTNLTKPVVQKQQQQKLSKQQQQQRDKNNQIIKQNIDYLNTLQKDLKKLLQDQSLSGIAPILNQGIENLNQDIQYYQKQQEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V8E2</accession>
    <name>A0A8S1V8E2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0560097</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000056">
        <property type="protein sequence ID" value="CAD8172122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V8E2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8172122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="88" mass="10804" checksum="36E97BF3D9953B8D" modified="2022-10-12" version="1">MIDKFHKFSKETPITYLFFLQLQKERHDICKTKFLDLFIRLKSLSKELYINRYSFDLGNSNFKQFFIDYQNQKNLFTFKQSTLDLKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VDU7</accession>
    <name>A0A8S1VDU7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0620132</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8175037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VDU7"/>
    <dbReference type="OrthoDB" id="9992527at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="2"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8175037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="431" mass="52142" checksum="F4CBD109FEA05300" modified="2022-10-12" version="1">MLKNQQSLYEFHVTCVWRNKQYKYIDKLQNHIYIAEDLQHNNRKIIIKQITDQYKKYREREKQITKDLIRLYKSNQYNPQIIEYYDLFEEDDSTYIVMQLCDSDLYEYYQNKLSKIQKNTQFYCKVLSIIRQIIEGYQYLIYSFGSGFLHRDLKPQNILLVDNQFKICDFGLSKNTNENPTKRIGTQKFQAPEMYGDNTYDNKCDIYSLGILLLWLLTGQYSKDYISQLNLNFQDLLTLMLDDDPIKRISWKQLFKHPVFVKEPIDFLETERLNQTSITFQDLSQIIQFADLDSTQSSFSPKVITSFIKQSNYVQQLELNKLISDTFKHVDESNLELTRILFALWIKIYDQEILQEKDELRRREYHKLKLQIQNQQLIRIKTQVDYRIEFLSIKELCHKLSYHMEHFKNDEDLKNRLENFLKNKMNQVQCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VFB7</accession>
    <name>A0A8S1VFB7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DDB1- and CUL4-associated factor 13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">WD repeat and SOF domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0630146</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat DCAF13/WDSOF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VFB7"/>
    <dbReference type="OrthoDB" id="10249065at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001105">
        <property type="entry name" value="WD40-repeat-containing domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007287">
        <property type="entry name" value="Sof1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051733">
        <property type="entry name" value="WD_repeat_DCAF13/WDSOF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22851:SF0">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22851">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA U3 SNORNA ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04158">
        <property type="entry name" value="Sof1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="61"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="104"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="280"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="330"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="Sof1-like protein" evidence="10">
        <location>
            <begin position="357"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="406"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8175617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="447" mass="51317" checksum="6C7F2720CFDE6559" modified="2022-10-12" version="1">MVKFNTICRSGQERSTNADITKVYRNPNPKVHPLAHAREYQRALVATKVEKIYAQPFLGALNGHSDGVSCIEACRFNLSKLVSGSYDGEIRIWDIPSRRTLIQLNAHQQMVKGVSFSRDGMRIVSSGEDKTINLYDFPQLLEQSNNTFQDPLMRYLSKGTLGNVDHNYQLSQFATAGQVVQVWDYERPKPIMKFQWGVDSVSCVKYSPADSNLICGTSMDRCVILYDVRAESAIHKVAMLNKSQCLAWNPIEPLNIVIGNDDSNCYTYDIRKIELPTMIHKDHISAVMSVAFSSSGREFVSGSFDRTIRIFPFNKGFSREVYHGQRMQQVNSVSFSADAQFVYSGSNDMNIRIWKVNASQKVGIINQRETNATNYRQALKEKFKYNSEIKRIAKHRHLPKYLMNKKKQRQEMKESKNRKQRNAELNNPGQYEAPKPEKQQRIEQFIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VFM7</accession>
    <name>A0A8S1VFM7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0630042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VFM7"/>
    <dbReference type="OrthoDB" id="304984at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8175411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="452" mass="53957" checksum="E7ED3FED2A621E2C" modified="2022-10-12" version="1">MRSFPLRFIAQRTTQLFRSNQSLFKLMPSRIIRYHYSQEFIHNNNNNNNQDQSKKENKTIDIQPQYMIDLFQKLIVLRKEELTEINQENMVTTLEQLVPFIENFNIHQLEYFLILANEIITVDNKLTDKLYEQNKHLLYSEQSDFNQSLKILALLIENPLEEQQLENIIKMIFTILEIPDLDSSTLAYSIYVYGLIVKQYKIESTEFFQSITRHLKKIDQDYIEIMNKDKKDSQFINFTQQDLKLLALGLHLGGCQNTDLSKYISIKAQSIFIEISNLIIISKFLLKQEIINEEFLIFFKQQLDKSKNQIQSDDILQLIIAIKQMKQPDQEILEFIIKEFLKTKDQFNLGDKAFLYQKMAQMHCKSQELWNQFGLEIQEITDKCEWFDACFMFYCIFDVFEYLPQNCQEHIFNYIIGHQNEIDGEMKELLFQKLQEKGIITDKDIEDSKQYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VFV8</accession>
    <name>A0A8S1VFV8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0610267</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VFV8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8174759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="64" mass="7885" checksum="AFBE8BE6CF33604B" modified="2022-10-12" version="1">MNFWIQIKEDYKFIQCREQEILENQDRLFQQTGKIRHGGVQVPDTLQKKSYQEFYQQIVQNYTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VHH0</accession>
    <name>A0A8S1VHH0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0620222</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8175199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VHH0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="80"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="470"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8175199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="518" mass="61750" checksum="B2BC8ACC6A4C537C" modified="2022-10-12" version="1">MEEKEVDALWNQLLKTTVEMFGFEFKQEYLSKSFNRLYSDYKMFTQKRNYSHFGLWLFGFYALNFLQSFQDKNISDIPKIIEQLIFCPILIMILISTKPFLYRSFESELLYFLQNTIVIIVGIMFPRPQQTEYGIFIEFFIYQANLESLPFKIIYIIVSQLIQKEIKSFMDLVVFMILLALAVYQEIHRYTSYIQDYEQVEKHRKQEKEDLSKTKEITKSQKQQEDKSGYCVRLYILNIKQFIQSHNKMDVRLLKYDIFSSPFAHKPSKGYIPKLADRSLTSLLKYKNETIKQPIHSKIFKPYGEESEIKTQQIMYTTSKLSYSSSVKNINKFPEVQLDPKAVKLLTQGRHFLKSDYQTKRKFQETKYNQHLKNHSSDYLMQNIQLKFCEKSVKKQFKANLIFFKKTKNIPAQIQLIKKEEERKSMPETKNDFYDSRQRKIKMPIPTKQQHTDHSMFSSTFRDNFLKSQRSNIHPKPVSQEKSTRQGHKKTQSVQTQSHLEEGNKEVPLFGNNYQPNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VIK6</accession>
    <name>A0A8S1VIK6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0680053</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000068">
        <property type="protein sequence ID" value="CAD8177728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VIK6"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000537">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="56"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="11">
        <location>
            <begin position="312"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="34"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8177728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="371" mass="43118" checksum="E49B8EF9DCF7A4E8" modified="2022-10-12" version="1">MGMCQNKPAEYTSKIPSFKDEMPEEDDENEKIGETTQLPIEGESTNSNPKLKLEDFDLIKVIGRGSFGKVLLIKKRDDQQLFAVKILRKKMLSKKKQQDQAIKERKIMSLINSPFMVKLHYAFQSPSRLYMVMDFMQGGELFLHLRKRFKFPEDWVQFYAAELLVAIDILHQSNIIYRDLKPENILLDKSGHIVLTDFGLSKLGFERNEMTYSFCGTPEYVAPEILYQRGHTFVVDFYSYGAMIYEMLCGTPPYYSKDKREMLKNRCEKPLEMKSCFSFYAASLLKGLLTKDPGLRLGSNGIQEIKKHNFFNGIDWKLVEQRKLQPPIIPKIQHVQDLSNFAPAFLQQPIMETPESISNEYFEGFTYQQVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VSV5</accession>
    <name>A0A8S1VSV5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0740052</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000074">
        <property type="protein sequence ID" value="CAD8180330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VSV5"/>
    <dbReference type="OrthoDB" id="550424at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000105">
        <property type="entry name" value="AT19485p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000357">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051339">
        <property type="entry name" value="DnaJ_subfamily_B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078:SF553">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="2"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8180330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="327" mass="37223" checksum="088116FC82D140B9" modified="2022-10-12" version="1">MDYYKILEINRDASASEVAKAYNKLSLKWHPKLSKLDHNTTYHHFCLISEAYEVLSDPIKRTFYDKYGEEKLKEGFFANGNLKGGYSFAGNPEEIFEKFFGTSNPFAQLIDTNGSENHGTLFSHAFGGQNFPGIPGPQDLEIQVECTLHELYNGCAKTVSYQRQVLNKDGITTRQITETKEIKIDRGIETGQKIVYKELGNEAAGFKSSDLIFLIKENPHPTFKRKGNDLLYIAKVNLANAIAADPIQIVTLDNRKLQVPVDQIISPKYVKMIENEGMPVFQQDEVKDFGKPYTFGNLYIRFDIQFPEDLTESQKNRIKDILLEGQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VXG0</accession>
    <name>A0A8S1VXG0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0740190</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000074">
        <property type="protein sequence ID" value="CAD8180572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VXG0"/>
    <dbReference type="OrthoDB" id="1630758at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20104">
        <property type="entry name" value="MBT_PHF20L1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8180572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="236" mass="28267" checksum="CC2D91E66B9404C6" modified="2022-10-12" version="1">MNRIDELRKVKFTNKLEQFLLKKRRAKTNLEEYQIRKQKQLTYTPQNNKDQICSLCHSFYVNYKKCPICRREFKEDFSYPCLSIDYWIGIAQKNNKDYEVKLQEISLWKERGKVKEFKVGMRLDILDTVFIWCIGEIKEIRYGQNDKPKQVLIHYIGWDKVYDELIDVDSFRLAPQGINTSQKNVIQYQQSNVETLQRSLNVSQNTNVREINHRSLIMSLASQFLSFRSDLFNNQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W2A1</accession>
    <name>A0A8S1W2A1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0800065</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W2A1"/>
    <dbReference type="OrthoDB" id="291446at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010504">
        <property type="entry name" value="AH_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50870">
        <property type="entry name" value="AH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AH" evidence="2">
        <location>
            <begin position="116"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8183073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="490" mass="57685" checksum="A3187E06CA77DAF5" modified="2022-10-12" version="1">MASFASRTGQKIKEWWGAKVTVDPQLETKKQKINEIDQYIKGLSQSFNNISALFQQMHGHMGRISSITQKLFERETDTNKRIGIEVTSMFGNLSELFRSKTEPTSELNKNYGEWFQTIETLKIQLANFSEVRLIYDHYKLKVDELEKDRSNALTKGQPEDFKLSDKIRRNQSKLSSSETNYKQKLSEILNNMNKLLGNYYRMINQSLDSFITINYDLYMNAKSIVKKNQKAFNKFKYPELEPIIDLLREQKEMSSLLEREQQREKELKELKDKEKEKEKEKEKNNNEKDDKQQQQQQQVPLQQTPQWEVNNKFNPFGQTQQMINNPQMINPMINSQTDFTTGFGINTIPKQQQFNINPILSQRQSIQSQGMNNNFAMNFPPNQNRVDIFDDVGLQMMSDLRASQINNWQQQQQYAQSQILNQNNFVNNNPFMNSQQLNQRPNLQQNQGFSQFDSQINRSQQPNPFAELDNETQRRAIQQFQDNQKTNPFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W5L4</accession>
    <name>A0A8S1W5L4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0830144</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000083">
        <property type="protein sequence ID" value="CAD8184600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W5L4"/>
    <dbReference type="OrthoDB" id="300002at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="50"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="219"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8184600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="583" mass="70437" checksum="21508F8609A84797" modified="2022-10-12" version="1">MSHKYYSISTKVSSSDNVKIIRIQSNKNHKSHNNLINPNKIDPETLSHQNNQLKSQINSLSDEVQVWKLKYQQLQRDSTQNLMFDNSKTFLISLKQQLKKLESDLSEKIKQIEEYKKMINVTKYQELQVELFNYQQQVLLQQQEIKDLKEQLQENQTFKIDDLYQKQEKIIQKLSQKNHELKSENTTMKTERYFQNGQIESLKTQLIIKEKEIKRLVSIQKQLEENQQFQNSITKSRQQIFRESIENAQLQEQLKNLEFRYQTDIKDRDQEIIDLKQQLIEYEELKKQLEKYQYISQEQKINQFDIQKVESRQDSTRKLKISLNNSGISQQQQNYSVLRQSSQQYKQSIQMDLSKIQNTEFTKYCNEIDESEKQLQQSNRKQKKVIRVNQYDVAQFGLEIKFKLLSKEVSIQDVEMLFEKYDQQIKIHELLDLLLQEPFKIQSYEQRKLIARYLIEDNNEDYVVYDALRSNEIQIVLSVFKQLIGKYELFTKKEIYSIENELKLIFEKYQFQFNDTIQQLIVKKKHLKPGQCEKADYEQAIKFSELNLTPRQLEYIYFINYTFFQDLYIIYYQNVMTYFNKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WA09</accession>
    <name>A0A8S1WA09_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0830210</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000083">
        <property type="protein sequence ID" value="CAD8184719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WA09"/>
    <dbReference type="OrthoDB" id="302736at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035755510" evidence="3">
        <location>
            <begin position="16"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="282"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="134"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8184719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="346" mass="38677" checksum="2FA5EE4B9420C7EE" modified="2022-10-12" version="1">MKIFILIVLLGLTLGVQVNEQATILQEALTNNLKATSMGRAVLAMVELGSPNFNPLFDALEAWASLIEQTIADENSSYGAFVQQVETEETNYKSLIAQYESELAEYNIQIREILKSRISLEEDLERTRTELGATKQQKANIENQQRQDSDDFKTRTNQLSAAIVVIDEALKVLDKAKYSSFVEEDAQQLSSLVSGTPELHSLLLQLNTEDYKSAQNLQKVINLLQNIRDQFNQNIQTLQAGFTAAQQQAQDLFELLNAKIDQLVKDVIPQLQQDIQGKDSEIDSRRQLANEAQANLDSARESLKQSIQEKGLATNSHNILISEYHSNLDTVAECISALHGSGIKRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WAA3</accession>
    <name>A0A8S1WAA3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0840021</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8184819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WAA3"/>
    <dbReference type="OrthoDB" id="309925at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8184819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="221" mass="26135" checksum="3B20FAB918056A65" modified="2022-10-12" version="1">MIKIITYSDIRLQSTSIEEISNSYPRLFIITLERNGNQLSEIKKTTISEMNKNLINQKGIKIFLIQKQELNQYSLFSIFKLDYSYLDLINFITNLIMSRTDGFNFTITQSGIQSFQKLNRKICIENNDISLSQLITDYLKKINLDDMSQKDCQNLLLFEYEIDSVQSFDFHSFIKDSVQTVKKMIQTIIQIFQSLEQLNQQEMHSQFEQELYNFLQELINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WCP0</accession>
    <name>A0A8S1WCP0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0870135</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000087">
        <property type="protein sequence ID" value="CAD8186557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WCP0"/>
    <dbReference type="OrthoDB" id="24670at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036157">
        <property type="term" value="C:outer dynein arm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036158">
        <property type="term" value="P:outer dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001467">
        <property type="entry name" value="Flagellar outer dynein arm intermediate chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002334">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF11">
        <property type="entry name" value="DYNEIN AXONEMAL INTERMEDIATE CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="479"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8186557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="650" mass="75708" checksum="8D45EFE831C38C1D" modified="2022-10-12" version="1">MPPKQQKVTLRRPIPKKQPKIANQNLNQQEAEPDGDESEYQHERERDRPQLTEKELNEDMPSKMLIPNNPQAPKNITQYDYLQRKYKTDELVEQLIIHFRLDGEIIHKDSNEARIQEELWDTKLALIKEAERNQEMEDPGASKDKEAIKQTMRNKFNYNAREAQTDGRVIRERGVSTEPPPSDTLKGQITQWEIFDAYIQNKAKEESQKKKDHTSDNTVYKPSFKRCLKIMERTVVHNDQKEKYNDYKYYWNQGEVVETSKNEGHLLPIWKFSNEKQKKKHVTSLCWNPRYMDLFAVSFGSYEFSKQRMGLICLYSLKNTTHPEYAFTCEAGVMCLDFHPQSPALLAVGLYDGTVLVYDIRNKHKKPIYQSTVRTQKHTDPVWQVKWNPDISKNYNFYSISSDGRVMNWVLMKNKLEPEEVIRLRLVGKNEEESTLIGLACGLCFDFNKFEPHIFLVGTEEGKIHKCSRAYSGQYQETYIGHNLAVYKVKWNNFHSRTFISASADWTVKIWDSKISTQIMSFEQGMQVVDAMWAPYSSTVFACATQDKIFVYDLNVDKIGKLAEQKPSKQPRLTNIAFNQRDPIILVGDTHGGITLLKLSPNLTKSGVKASNFPDGKIPKEFESMSLEEYEKQKMENLLQVVSKWEREDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WJ58</accession>
    <name>A0A8S1WJ58_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0940044</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="292036at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15431">
        <property type="entry name" value="FGFR1 ONCOGENE PARTNER/LISH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15431:SF4">
        <property type="entry name" value="PROTEIN TONNEAU 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2023"/>
            <end position="2042"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2054"/>
            <end position="2077"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2126"/>
            <end position="2145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2227"/>
            <end position="2249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="932"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="937"/>
            <end position="946"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8189473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2325" mass="276552" checksum="BD50F21B0C8BB8AF" modified="2022-10-12" version="1">MKQKLTFSFVPSIPLDIQIQNLRKKILQDQNLKKICFKEFQNQYRIDHLLKVQSNLRQINTIQQVKFSSTVLSIEHFALLAQWLKQLQCNNFIIKLNNNKVDNNEIHDFLNAIVKGDIQIKKIIITNNPGIYKGQLISELKQLIRQQNANNYQYQYKLVLNKQYLSLAIIRQNFNETQQQRDFQEQKVFLKISKLNFFSFSFNSTFTQINFRALEQLINLNFNENGIDQISKLQISSSLIVNSAIMCEQISTSKGLLKMVMQSQSLLNYSVPIENLMGNRYHKFRVNVLDIEKNSTILEKTYKILSDNIFLHCKQIKFSSSLPQQLSTLYKMFVMTESFQQYSNQLIQQSIEQGKILKQNLRVLDLTSLENYNRHDLIAKFLQAFVFTETSNLKKLTIQNIDIGRTQAYIQALIRFREYIQQQQQKYPFYQNFKLPLKSLKLPNQTYRMEIATMKQFFQEFFFTRVGDILEIEEFIMEKCFRQSNREEGLKENCAEIFQLLQANPGIRYSIKRIKFIESTYQFDSVTYRCLFLNHCFNIEELMIKKDTIAGEFIQFVNETEQFFLNQPLDYKLPLQIITLDDIEDIVFNISDLLQFFVFHDRMKIKKLVLKQIVLLYSQLSINKLEEVINSKDKNQILSLEEFEFSNLTDDVGVLQFFKYVVFNKNIRIKKLKFGNLDFSACVYFLKEYFETQNELNLNHSLQDLNYLKISNVNFEDEMSWEFFLQKFVLHPTMKLKSLKIQQQKLDEAFYQGISQSFENSGEILKLELKELKYKKCEIMVDPLTAFLYTCSLNHLSFDSCVKMDQGLKICMDNYHQSNVKQYNNFKLESFSIKMSKVEDTKIFKWLIKEIALNIQHQQLKKLNLSDCNLSNEHLRILQEQIELIKDNNQKQKSAFKLYNLKIDQNQQISESTWIDFFNVLMKNKAIVDEDDQNSEKTNINQQKHNNNQKKKLKQTKLINDKEQQLSINRIKIEDQQDREIKEKIDTLQKNIIESTLYRNDLNLKQKIINNYHIFTPNFPKQLKQLNLNMNFQELDQNDKTQIYERLISGLIMHPENQLTDLTLVNLNLILFMEKCEQFLIYYNVYQNQCIKNEDNKWSSKLESLSLKSLATQNQKSTDQFVKTFICSQSVNLQKLLLENFNEQLIVSLMNQIDNRQDYKITELTLLCIYESIECKYFNSFVDDIIYCQRFPLQKIELIGMGIQSNNKQISSQNKQLKQIQYKQKQISLKQITVQLIDDQQVKTFISILGHLLQQPDCKLEVIKIQTKAQQENLSSNFFSQLTNELKDIQELNLKEIHVQGKVIFDETFVQFLDKCILSLEQLSIANVDFPYSQKLKDKLNKLKRKEKKSKFTIEIKQIATGIEHFFECFTFNKNIKATNIILSNPQNFTSLRFENMSQFIKSIKLDMGLLYSSTVKLNRDSLTLLSQRFIYNEQSHVEEMLLYQCQLKREEIEAFCLPAAEFREQIIQQQRQGICQLKLKQFSIYYSLYIGEVGCEIISKDLIFFEYIDIERIDFLVTNFNDQMCDHFSQAAKNWLTFQQLHNRKNKDKYPLKYLDIGRNEFVQSKTTWANLLQTLVFTDRAIELSTLNLQFMGFNDETISYLVEQAQLYLNQKPDNYVLPIKKINFSQNNALTHRGWQNLFDNFFLHKKVNLIELNMISTMLDTQIKLNTIYECIQKRALQEPNQKLQLQVFLCYNVSLKNAIEPYLQVPAKDYPKPKNLPVDIDYDSWKYGIFDGIPEEFGSIIMLVNTLIQKRNEFIETNKWKLFEDIRFPNYHLNFHQHYLAILNSYLYQQNNKLANLYLNLSTLDIMCQFFNYTTFIRATPYPYRLIFSEESLQYLSEQQIKLKKLKLIQIPFAKLEQLSQLDVLQIWQQIRQLNIQIQKIEMEYYLNDELIEEMFRQNYNESDLLDLITLIPPAKIRIENTLTLNTLKGFYSILQNQYYFSSSIIKYSFNDWINTGLAYQIREYAYNYVPKTSIQSKLKTIAYNISNWLVMPQQTYIFYNENQSLQQYLDKTATPLFIISLINMFYLILLIILPLQQIFIYSINHELNFSLVNSFQFIILISTIYELYLINNLNQKNKPKALQQILFRQVQDLPKLVSQKCIPYCNAFSIIMYYSSDSHIIFTIFIIIYFFYYLQLLFQNTTIMYQYYKNKKVILQDIYHLSRIGKFSEIEKIINEILPYNSTIIKKTKLTKNILPQSIYGKAIHNKIYFNLRDFILKDLPFLILNTIFIISQLFMNTLTLYQQLSQIGQLVQIYISFKNFISYRPSVVSQDDFDVICKRNQNNYKRKKLNLLQKEKKQLDLYANIYNEKELKNKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WKJ8</accession>
    <name>A0A8S1WKJ8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0920041</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000092">
        <property type="protein sequence ID" value="CAD8188689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WKJ8"/>
    <dbReference type="OrthoDB" id="286671at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="124"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="252"/>
            <end position="253"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="8">
        <location>
            <position position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8188689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="568" mass="67524" checksum="4516F96CD16D5D93" modified="2022-10-12" version="1">MQSKTFRSFFDEQTIDSSFWQKSISQINIEKIEHQFMLFEKSQKTGFWPNKQTIHQINLNMSRIEKIKYQNDQEKESCNRKKYGIRIIRNQRCREFYSRSKEINQKIWEELKKQSLQITFKHDYDIQKMIGKGNFAKVYLCNKKSDKQQFAVKVFEKSKMINTETDRLALLKEISILRKLNYKGFIKLHEVYEDETHIYLVQEYLQGGELYQHIKKNQKLPENIVAGIIATLLKSLEYLHKKNILHRDLKPENMILRKKGILDDLVIADFGLADFYDPFGNYMFQRCGTPGFVAPEILQDKLYDSKVDIYSVGCLMYLLLFGKSPFKGSTYDEIVMRNFNCKVDYQSIESIISIQCLQLLKLLLNRRPSQRPNPKEALKHEWFMLNLDNQRYRELNNNEEQDQFTKEINKQILNDVRNCGYMKYFSGIQTYEKPEYSTPQISYVNKKQDASIYTPQYAIMQSIHEQFKDSEKKISIRNLEDEFGDIILEKESPQSHKLPQYQLIGKLKKVLDQNHSSQQHSIIIKKQSQNQIKDIEHHLLSITQINQNQTQYKIVNKIKNNLKNLEQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WL59</accession>
    <name>A0A8S1WL59_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0930168</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000093">
        <property type="protein sequence ID" value="CAD8189351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="300476at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2553"/>
            <end position="2571"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2608"/>
            <end position="2625"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2664"/>
            <end position="2685"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2697"/>
            <end position="2718"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2748"/>
            <end position="2773"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2858"/>
            <end position="2875"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2887"/>
            <end position="2905"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8189351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="3021" mass="352373" checksum="B070137E3B550A49" modified="2022-10-12" version="1">MLQFAQTSFSNYEDSILTSGFLMTEESINIINQDYKDTYGIGFWSMSIPLQIPKRNFDIDIPYDDTQQETGEMLLLVKDSDTNDYLFVVSKILDYPNQQVQHMIKFFNKNSHFNFFYNFNALEYEGIWIFHLILIQPINEVVLVEVSNQSKQSHIFASEIQKNLKIFQGGRGQINDQNLNTFRGLLSKLIFLPYLSYSENSFQQIQLDNQIPQKYQTEQKIKIIPDLLTFDGYSTIELVINQYGKSYCLSGWVKYNFQNSEADKYILMRMTGFINYEDQKKLGDDLFSVQVFFSKIQPQKTKIVVHADAYSMPIQQSFQSQYDLTLQGVQDENYEVVQSTRTLQYQEDFRYYEGLQQWHFVQYEYGRSNFDEKMLFQIQFFNKLGLIREQLGNDIFRGSFINSQFNVFFGGDNFNHNFLQAQVFDFKIEYNYDKDKVLLFNCHYSCLTCKGPQDTDCLSCGENTNRYFQSEVSMCKCNQGYFDFGQIICTDTYYSSVQLHEVATQNANICPLGYFRLPIDSTNYECKQCPQHMTNQFILCAECYFFQKTWYLKPVCKQDFKTLKDSQDQVFHLFTRNKLEYDLYSINLERELIFHSELEDYCVKNTTSKQFCFTFPYYHLGNLAQMQCKSNYFIDTDKYQCRQYKKNCTVYDNIGNCWGCFPGMYLFWRSCIECPIGCSTCENKFGFIPFCQTCSKYYSLIDEQCKMCGDYCEICESYYDDYLEQKYLRCLKCIDDSKYHISLDGKKCLLNQISNCVHAFQALRDDLTVNTLDIKFKPKFDLDQIVTLCAKCETSYVFVFEINQCVKDITEEDCQVGIGQLKEDQSLKSIICLSSTKQTNQVVQFTEGCQSYNRYCQVCLQTDILNAFACLDCINGYYLQIPSGKCLACPPELKCKSCYSQHSILKDRWKLQVRSFYRKYIEINGFHQYIVYGQSQNSNDYEIICSACLDGYKLHNNVCLKYCQDDCLECIFSNDKYICNKCPQEQRGRRLTLIDNKCVECPENCALCRVRSNEEIQQINPLFKNPKYNKYTYQCLKSYQDQSYYYDEDFGLFVECQKNDQGNGCYKKLIISLYLYGDKQQYYDDFFGQPDEESMIKFQKENILLFDLLESEYSSFMEFENDEFYTLANSKLIKTIVIKLTSKSEISYNNFAGGQIKQTFQENIFTLKDVEIEFNFQFDTIFILHSDIEFINFSKITLSSITLTSISISRFIFRSSFPQTILLANINFLKNDYINDRDFNFSFNFKNVTSLQMNNLYIYNVSIITQGSFINIEETIYPKKLIFSNITLRKCKVETSFLDLQLGEQDYVEIDTVTLISNFTNSTFIKGSQSIENGKLKLSNLILTSYISNSQYLFDFEKMFRLEIINLEIIQTILINTTLILLNRNSYLDNVKFKECQFLTKSIGIENHNNFLSQNITQQFTSLLFEDNHYDSIIKFIYLKKYSSQTQNITLNYFSQINNHYIKDSINEYRKLVDFSLITIQYDYVSISNLVIERGFGLNDITIYDCQKLKIENGIFKQEKYRFLGLHKFLSCQLKQVQAQYYSTTLKIFSSKVTQLKNLTFSKVLSYNFPIIAIDSADQKLTNQSETIIFQDISFSSNIILLTEILFQTSLISIQSYQQSTIQINNITFLNNVFHQYTQFDFTQTAGLLLIDCPYCQIYLMNSNFQNNIVLNSISSIMNIKTQKLQISNSLFYNNSIFRYEIIQPHIQWGFTSSVSEYVIKQIFESNSKSGVGELQAEEILIQNNTFINSSGSIGGCFSIFSYGSTDILIKNNSFEGISTKFLNDLEYGGAIYIDGSSSSSLNIELNNNIGNKIFCRGFGGFIYLKSITSQSNLTIINLHLEDIFAQQGSAIYVSFSKFVESPQILLVQKVQISSTQIGYLEFLNKFNEFSSQQEIQQLINNRSLVYLEFGSLIQMSEIQTNNLILESFLILQETKYIYLSQIQIQNSSISNKLISVFSFTTTNQEIIIQNSVFRSIQIGFNFTNQTNTTCININPQLNDIMYQCLHDFIQAPIQLNVNQQSGYYNQAFCVFQEIIKQIDYQNSGLILFNNFTNQDKIQISSLTFVNIFCSICEYGLIYLQFQPSEYFIQQQLITSLEVRNCSCGQQGCLNIVKLPNKDRRLLNQFELYSLIYEFKIRYYNCQHNIATQGTCLKINSLQTLLQNSLFQYNEAAEEGGSIYIKGNKNLIIEDCVIHFNKGSIGGGLYFSDQVAMNLLNNKTKIIKNIARIYGNNVASNPQQLSIQIRNDSQVLSAKTKIQNSTLKIQEIKLNPYVDLNGQIVKHIYLPTGQEISSYKIFDWKNKLYYNSNMIFRILALDGDLNSVQQLQNSQCIIESRRFNESNNEDQDFTNNYTSIQQISFNQVTQSYNLDDLIVYFDNSVPKEIVLQLQFSCNSIKVPIYNTQYPYQVIDYHSNYKLRVNIKTYQCQFGEIKNTTSFSCETCDSDQGLYSLTLNSQKCDIKDDLTTLSIKDNQLILRAGYWRPYFDTKAINYCLNLQENCLGGINEGDNSCYIGHIGALCEQCDLYNIRGNGQYSIVKKFTCGPCVEKEKNVFVILGIIILTLIFLLISIQGNRKTMEEFTKSSVFKIYRYQVYLNKNQSGTLIKMLTNYFQIIVSITTFQLHFSAGLNNTMNVVGNPLQTSSYSLDCFLVEYKDLQIEYARMIWQIILPILYIVVFICFYFLAILIKKDKFNPSIAATAAIYIYIYVQPNLINGFIELLSYRNISGFKWIQANVSKRYDTTTHTMWMFEFCLPFIILIAIIIPCFFFFGLYSNKNHFEKKKVMSHWGYLYNEYKKEAYFWELIKIIQKELIILSLVYYEDTVVVKGVLVLFITYTYQELNALYQPYQLNNLNQLDYYSANISMITIGLAIGTYMSQQVHLEELQIPFFFLMALQNLLFLGKIIKQILQEYSKQYEVLLDKIKEMIKQRFPEIDENPFMKRLFKSRGESRKNAILRFSKLRNFGIPLAQQIIQMRQANFDQNAKKRTSTSTAQDGGFQSKKKGVYYQKSLLKIKSFAKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WNL6</accession>
    <name>A0A8S1WNL6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0990119</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000099">
        <property type="protein sequence ID" value="CAD8191598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WNL6"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000592">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF206">
        <property type="entry name" value="HSF-TYPE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00434">
        <property type="entry name" value="HSF_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="5">
        <location>
            <begin position="116"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="272"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="181"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8191598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="328" mass="39331" checksum="D87410F91CA2AAB7" modified="2022-10-12" version="1">MRTEIQLLSLFLHKLIKSKLLYIIFNELEKQKLSLYFKRQQSYNSTYSQRNFEFSLKNLLNGYELNSGKLHYEPIYQQFQYKLNNQNPEFENIISFNSAGNAFIVKDQNGFSDYVLPKQFKHQNFSSFIRQLNMYGFKKIRNVNNQNQFSHHYFLKDREDLLQKIIRKNSVIQRFHYIKLQNKAKKEVDQLNSNYLELKTDIEQIQKELIYFYQQFDQFQSSLSTLIDSSRQQIKELSIIKNQNSFCNDLLFQVLSNVSKSQPEQNYHFQYPFVNSTQQTQTTKSNRSDKDELNNDNENNNNSSKEDTNQSFQSNSFREPQQQQLKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WNT5</accession>
    <name>A0A8S1WNT5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0960072</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAPRE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000096">
        <property type="protein sequence ID" value="CAD8190347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WNT5"/>
    <dbReference type="OrthoDB" id="2119228at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1430:FF:000011">
        <property type="entry name" value="Microtubule-binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000028">
        <property type="entry name" value="RP/EB family microtubule-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004953">
        <property type="entry name" value="EB1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027328">
        <property type="entry name" value="MAPRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10623">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN RP/EB FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03271">
        <property type="entry name" value="EB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51230">
        <property type="entry name" value="EB1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="11">
        <location>
            <begin position="4"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="EB1 C-terminal" evidence="12">
        <location>
            <begin position="165"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="112"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="246"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="112"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="135"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="249"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8190347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="268" mass="31130" checksum="D59EAC3B4D207F55" modified="2022-10-12" version="1">MNTEISRAELLQWVNDTLKLSLQKIEQLGTGAVYCQLVDAVHPGKVALNKINWKAKQEYEFVNNFKVLQQAFSKLGIQKPIEIEKLTKCKYQDNLEFLQWLKKYMDQHVVPKDYDPQSKRGNAELDDQPAIIPKRNPERSRTSIRRDTFNSPNQLQSSSSIPKTNSFAHMEPYQKKQPSMQDLLIQIETLKQERDYYFQKFKDLDFFIEGASQLTQEQMYKGIKDILYYTADKSVIVLPNGELEVQGLPPSSEEQSSSQDVMQQEIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WRR0</accession>
    <name>A0A8S1WRR0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0990052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000099">
        <property type="protein sequence ID" value="CAD8191470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WRR0"/>
    <dbReference type="OrthoDB" id="310725at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8191470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="137" mass="16743" checksum="D2FDF31DA99251D2" modified="2022-10-12" version="1">MHNVSQFNEKQLCIRKILEAFLMFQLSHNSQEKSVQEIIYTQYSQLFNGLSNQFFICLFIDYQKLALFYLLILSVFFNQIQKKERELFYTDSKRNIKFIPNYQIFKYEFLFLINKFKLAQGVQCLKTHSKKEFIYHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WTC7</accession>
    <name>A0A8S1WTC7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Casein kinase I</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0920198</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000092">
        <property type="protein sequence ID" value="CAD8188976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WTC7"/>
    <dbReference type="OrthoDB" id="300156at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001186">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="14"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="284"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="289"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8188976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="448" mass="52087" checksum="6CA7E1EEF983D3E5" modified="2022-10-12" version="1">MNQIQQGDVIDNQYEIIKKISQGSFGIVYYGRCMKRNKNVAIKVEKQEMASFNSLNREIEILTLLQGVSQIPELYWHGQFKKCNVMITRLLGHDLIYFQKQYQKFSTYCVYNIAFQLLWILEQIHKRNIIHRDLKPENILSKADTDKIYLIDFGISRNINQITNKKKKISFIGTSRYASLAAHQGIEQTAKDDLESLGYLLIYLMKQRLPWMNIEKNDPERLDKIRKLKQETKVEDLCQECPNSILKFMKYVKGLSQQQKPSYAMLRGLFLTTLHNISDQGLDWTNQKSKSHKQKSNKKQKSTQNSNYQSCGTIRREFLGEHLLPQNYKTFGAQDELVGSSDNGSSALQFSAITNQGSMKSIGVGFTVSDLHEGQGIQKHNNQIARISTFEGIVEHDPGMTLKAEQQLMERENQDLEIKYNLLHFKSVYFNFKNPIQQWILRSNYIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WUX5</accession>
    <name>A0A8S1WUX5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1040176</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WUX5"/>
    <dbReference type="OrthoDB" id="286864at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021258">
        <property type="entry name" value="Epiplasmin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10992">
        <property type="entry name" value="Epiplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="265"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8193593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="284" mass="32448" checksum="EC562C95D0429C57" modified="2022-10-12" version="1">MSQRPPAPSNQPPQQRQAPQQYQPPQYAPQYAPQYAPQYAPQYAPQYSPAPLATYPAQYAPAQYAPVAPLTYSVARPVAPVVAQPVVAQPVVAQPVLQQSVIAQPVVQQSVHATIKGESRIEYVPYQKAVMEYEEQEVVQYVPRERKVTDYYAVEYQTEYVPQVFQEKYTEYVPVDRYQERVEYYPVERQVVHQQVVQQPVVQQVVQQPVVQQVVQQPVVQQYVQQPVSVVQPVQTYPVQYAAPIVSSRVIPSYPTYPQYPQYQPAPQQVQQQAPPRSNLNNNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WVA4</accession>
    <name>A0A8S1WVA4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1010059</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000101">
        <property type="protein sequence ID" value="CAD8192275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WVA4"/>
    <dbReference type="OrthoDB" id="286817at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8192275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="108" mass="12576" checksum="195536411A9DAE77" modified="2022-10-12" version="1">MDYINFLKEDNAVDQVLIQFNEQIDSTFQQQNLQEQVENLSSDLSKPLIIDEKVYYTVHQTPWHLVTQKGGCCIIKKDGVLVIASYDETKGQNPSRFRRRMSLLDKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WYS9</accession>
    <name>A0A8S1WYS9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1080091</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000108">
        <property type="protein sequence ID" value="CAD8195033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WYS9"/>
    <dbReference type="OrthoDB" id="298019at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8195033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="129" mass="15359" checksum="57392E1D02A3D1E5" modified="2022-10-12" version="1">MISFQDRFKGKMSYISKYGINQSPDVKQNSYILQQDQSNNKSPQKIINLNSKSPIKNSYYYKLPPIKNKIVDRTQNFNQQKQQISTKKIYSADHSKISLKKYDSDIEEIQMKHLQYLNKIEQLYMLQKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X0X0</accession>
    <name>A0A8S1X0X0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1110049</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000111">
        <property type="protein sequence ID" value="CAD8195958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X0X0"/>
    <dbReference type="OrthoDB" id="293931at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="416"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="456"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="525"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8195958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="572" mass="65270" checksum="2A2820EA5119C072" modified="2022-10-12" version="1">MLPNSYVQSMYINPQQQQVAPLPFGYTTNTPTKPVYQPQPPIETEYQMQVQPEPYREQIVVQQPMPNKTITLVKPKKYSYLIDWLLYILPIAFFIIILLIMALLFASKVKHFNPEGAHNTLETLKNNMQLTPIEDIVVATPSSSASALNMISHYTHDAVDCPDGYGKAHLGQWDGVSSGCICENGEISHSLTCFYKSNCKRVKSHDPQLFTTWQQKQYCTKLYAEWKTLEGAACETSYKQCGNVCVPQSKNCPLSGLLKDNSRQNDRNAIKIGTDNYIKQFENSSPIVSIEMIPGIGQIDSSPCYNHKLNPKFQSTKYYPLAKRPEMGCDNYKDLQSHRITLNTFSAHQTYQQNGLADVLSQLPFYQNYEDNSDTYALEAIKKIQINTNEVCQKLSPKDIDQISKSGQRVYKSERAMSLIIIISVGVILFLVPILYLMKNRIFQWIDMTEFHQPKFLCGIGLIIAILCIGLGAVYLSEVDGNNGLREHNAQFSKYLEKNCFPDDGLKLAVSQVNEFAKNTYFNTYSLVIAAFYVSIIFIVLLIIFVVYQYFAHKSLFDNPWTPRQQEYSEFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X3B3</accession>
    <name>A0A8S1X3B3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">STOP protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1080102</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000108">
        <property type="protein sequence ID" value="CAD8195055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X3B3"/>
    <dbReference type="OrthoDB" id="294559at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8195055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="419" mass="49324" checksum="0C4B0645004DF354" modified="2022-10-12" version="1">MIPEHIIQKLKTKQGHYSIDVKESFIDKQRRLSEIERQTVLQKKHKEMQLFRQTFSSSNSKLSKPGKRIMIKTKGCSQAIPLEQFQQTISTPLPPTPSSNIGRKHHQSLSQSLPRLEQENNYTTTLRKEPELQLNTQQDTHNQLEQQNQNMQTEQIEQMQIQPSEENNNDHIQCMVEQHLSDYQAFVMHLAGKCICVQCPCGKCKCKFEYQPFKPNISWKSHYNQEYKETQMKDQELKMNLNSIGRYQTLDLNEFKTTMASDYKQCDPFPNYVIEKQKYQANYGSTPMTSYNKFYMDYGDLHHEQFKQSHYKTVIPELKFNSSTTYGSEFKTIKQVDTTSQKPPNGKPFPTIDLFLGQSQNKLVHDQKIVEKCFQVKNFQEPIQTIPAFDKQFVSMTKKDFVMKEIPCIRNQYSQQQQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X712</accession>
    <name>A0A8S1X712_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1130127</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000113">
        <property type="protein sequence ID" value="CAD8196755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X712"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8196755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="44" mass="5328" checksum="74728E7FF58F47A5" modified="2022-10-12" version="1">MKQDFHHKTIKAFTMIIFLVTLMATTDFQDENQNEFTQKLIYDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XAH3</accession>
    <name>A0A8S1XAH3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1170107</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000117">
        <property type="protein sequence ID" value="CAD8198107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XAH3"/>
    <dbReference type="OrthoDB" id="290044at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045081">
        <property type="entry name" value="AN32"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11375">
        <property type="entry name" value="ACIDIC LEUCINE-RICH NUCLEAR PHOSPHOPROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11375:SF23">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_118, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8198107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="203" mass="23980" checksum="12CD15126E6FE911" modified="2022-10-12" version="1">MKQALIFKHESPHVKADFHENDTKDPIKILQSIYDQYDAQFVEEIILEELEIPEITPELRDKLQEFPNVHSFGINRCGLQSLTNFPSFKHLVRFQADGNLIKAKQIGYLKKYTELRSISLICNQIDDPKIMIKYLKEMKIIQLNLYGNPMQDYFKLFFDEIPSLIYLDNLQKTGAEVYYDYDDTNELSKQDGYICPNYKEYKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XB03</accession>
    <name>A0A8S1XB03_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1170028</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000117">
        <property type="protein sequence ID" value="CAD8197971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XB03"/>
    <dbReference type="OrthoDB" id="406844at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="70"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="115"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8197971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="333" mass="39246" checksum="AB440BB54303CC0D" modified="2022-10-12" version="1">MNQKKLQINQSSRQSNIIKYQLIPECTISQRELCLAIAIDKDCSKLVAGCKSLIKVFEFNQGMIKQIQTLNQHKDFVKTLNFMQKSKQFISGSFDKSIIIWKYNQNSQWSCQQILNEHNDSIYCLVINTNEDLIISGSRDNTIKFWINKNQWFHQQTIKDHCQSVYTLSLNQQQNRVVSCGYDQYILIIEQSEQNKEWKLIQKITIEQFGYRVCFIDNNMFAFSQNNQEQISIFEMNNNNKQFIKTKDIPIKSGLHFYSSFPQQYINSKCILVSKNGEYVNLIRKIQNGEFLTEQSIHFGISSLFGTMSNDGEYLVTWDKKSMQIQIRRYQEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XBZ1</accession>
    <name>A0A8S1XBZ1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1190063</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000119">
        <property type="protein sequence ID" value="CAD8198564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XBZ1"/>
    <dbReference type="OrthoDB" id="302096at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8198564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="658" mass="78670" checksum="78E7E605A2A87C8E" modified="2022-10-12" version="1">MMDNSYDHWFSKYDQESFEKDFLKVPYYEFLNQLIVNPDFSLLPNEPQLSLDNVHKVLQQTIQKNHLIISTFILNLLDNSKEPFLRWVRSVIAFIDCEKIYSIMFWNLNFIPNFLEYRLYASFIRLYKFLISLNQNVIQPKSLITEQIRLSNKFTIKTLAERYQDALYVSFDELAKLQRQMLKVLKPLVSQNPTLIGKIYYFIYHMYDKPTMQLLKDLDQQFGQLENQWVKKILIENELAKKYIDAYLNDDKQGGDQFEFEDSEILKQLMSQNKQENLYKVDAQDKSLLEKSKKTQFLNNLDNKNKRRNHDGKSSVFVQNYSIVSDLTISSNENRSKIYSEDVSNIKYNNVQSNLTSEQYTFNQYSNNNLQNSIIGFLASDLIHLEKEHQQEFKNNKLQKKIQIDDSIKLNDDLVEFISKADNQISIIKDEDILIGDNYKRKKQRIKNTKNIEENESPDVHRKQTLVNTKQHENHEEELRKVRARRLLGEQNQLFNEKHRYANNQDNQLSTDKDIHRYSFEDSISEEDDESEDEIKFKQQQTKVIIPINQSQNTQGIIFDKFQQYSGLYIQGGSEKNKYFLNRLFHMNENSLRISGKSLAPDFEERKWEFLKQSILPQNVIKEENYVLNFDEQIKPIFDRQYKFKSKLKSNYEKYYYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XGH1</accession>
    <name>A0A8S1XGH1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1230113</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000123">
        <property type="protein sequence ID" value="CAD8200083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XGH1"/>
    <dbReference type="OrthoDB" id="286354at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd19756">
        <property type="entry name" value="Bbox2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="73"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8200083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="420" mass="48560" checksum="8604F91ABE7E0C9A" modified="2022-10-12" version="1">MYCQQGGYHKDEHLEMVCLHSTCIVKGEVLNCVVCIDERHKGHKMIPLQKLLNLIKKQESLIMLNTFHQSQHLEMQISQLNKLQEQINQAIQKQRELIEQQLKFDKIIISYQLITSINEEKLLGQLIQKLLLKLEYNPRAQQLITRTESLKEEQPQINQFLTNLISFKDTLQISNPDTQLVQASRTSSTYRQNRNNSINTEGYRTMRSFDRSPLSPQPFEDRLSVTQSLSNQYYRPNSQGKQIIGEPSPPPPMREITYKFHSEYHGVGAQVKGKLVKQILNTDGNLALIGPSIYGNGLIRDSNLKLLIRSCQNQNIRYPMALGVCDQKRVKEKQYQKDSDGCYFLNSYGYITCENQTKIKCGKRFYNGSYVELKFKPFQSELHITIDDGEVIIIKITLNQSKLIYFAVRLGDLHDAIDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XHA3</accession>
    <name>A0A8S1XHA3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1250010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000125">
        <property type="protein sequence ID" value="CAD8200546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XHA3"/>
    <dbReference type="OrthoDB" id="2157380at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019332">
        <property type="entry name" value="OSCP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21439">
        <property type="entry name" value="OXIDORED-NITRO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21439:SF0">
        <property type="entry name" value="PROTEIN OSCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10188">
        <property type="entry name" value="Oscp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035876056" evidence="1">
        <location>
            <begin position="22"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8200546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="380" mass="43607" checksum="B7C37B67CFB74CF5" modified="2022-10-12" version="1">MNGAYMANGLILLNLFGDVLYIIEQRLRAQGIDKEKGLTVLNDLAIKIYTYPNLQKIFTPAKTLSNNEIFQQVDQVVHCSIMKLNEDSLSKLFDLVIMTLKSYVMKSNSPYEIYLCTLRHFDVIQEIGINDQAIQAVLQCKQTFIDKYSKLSSFEYMMIRGEIMKLLGGKFTRVQMFLNDKSQNQDGTFQQRSYGLSGYGVGQPGVIRMLKIPEKYNVVLAVSSQFKENKSTNPFQGKCNLGENIFQNKESQQQNKSQSILSTQNFYKTDSLDSQSSKFQTQSLNHLAQLIQAKPIDQQEDINLFYKPTAIKQNIDNQQQSQLINQNQPQQQSQILKQNIIQSFDDTNNNLQSHLLQNDQEEEEEDEIDLLTLMDKAAQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XHA8</accession>
    <name>A0A8S1XHA8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1240142</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000124">
        <property type="protein sequence ID" value="CAD8200443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XHA8"/>
    <dbReference type="OrthoDB" id="308383at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045606">
        <property type="entry name" value="ATXR3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46655">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE ATXR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46655:SF1">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE ATXR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19633">
        <property type="entry name" value="SDG2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="1">
        <location>
            <begin position="264"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8200443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="826" mass="97807" checksum="6469C3DC7CB525CB" modified="2022-10-12" version="1">MIYSQQTLQENKEMDLQGIDASEHPLAQLQSLKTRQNQERQEYMLFMQEYIVDCLTYENQEIEDDVVQTIKIICQKMVLKPLWNYEDLIVELEHQLETQTHDYIVLNQVNLIISSFDAQYQNKFNQISTHCLQTLKSLCSTEKKQLKRMSHYIEVQELILNQQDRVEEIHQLLTSIIQGQDNILSIDFWNYHPYQYVNTIQQDLSYQVKKQNVDVILIDTYGINQEVARALFQLLPDYEVILPQLLSTINLTKGNLQHALSNLYDLQLKTIDQLNPFTLKGKGIVCCQNEGFAGNEFICFYFGEVYTPQRWFEKQTIFHKRMQDGNRKTCSQSPYAEFFVNDDLLFIDPTRYGNMAQHISYSCDPNCKLVTVIFNQQNLLAVITSKKINYLEELTLPFPQTSIDQCLCGSIHCKRGQQLEIENTHQNSIYPNYIQRNVILLQSTLMNSQNTQNDIPDWLANWQKLNNQQNYINIQSCVDKVKFALLHLKTMQPPIFLVTNIFNQFWKNYEFNTQKIQLESSIINEIVIFLKRHSQLHQCIIGLEIIKQMKQIIDQNNIYALELTRMLLLLLSEIILNIDSCSFNNKAFATILYFMSFTHTYFSSTQYQGFDGKPFEENEFEYIPQPKNKSKLALSKQYTPQFIWGQLINWNKQTLQNPQSSMAQERRGVLCYPSLLLSFDNKHKTFPYQCKTREIYLEYFQQKKEIQPDLSTWSYKNQHNIYGTIFFEQYFSQQIVGEDFVSQICKLGMQTFEKKFRYWLHIENCFNMNQQMVDDLQTIYQEKFQTFFDQTPKSNSCTEYDNNNIKKKVKFNQDTIIAEETQFSTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XHB0</accession>
    <name>A0A8S1XHB0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Exocyst complex component Sec6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1250113</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000125">
        <property type="protein sequence ID" value="CAD8200750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XHB0"/>
    <dbReference type="OrthoDB" id="289912at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051601">
        <property type="term" value="P:exocyst localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010326">
        <property type="entry name" value="EXOC3/Sec6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21292:SF1">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21292">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT SEC6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06046">
        <property type="entry name" value="Sec6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8200750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="753" mass="88881" checksum="C306FA24BA904855" modified="2022-10-12" version="1">MYQCQKVFQNEEEAKAAANLIIMEILGNPSYQEDEKFDKLLNQFIEQQLELDKKIQKMIQSQIDALNKTKNFMDQCFNNVEKCEQQIEILNSEFDKDAQFHRELVSYEKIFFYRKNIGLMLDQMKYIFRVQEEIDKMNSTFQSGNCDYENLHYKLIALVDIRDNIIEAVNTSPQSKQDLSIILNEFKCLSEFENKFYDKIYEILLTTIEASIKKPQQLIKTLQIIETADKSRELRKKELIYKKRALQTIEQGIDEKFNQKLKDAKDAVQILEQSKFSSADLILAFDHTVKCFPKHYNIFKVIEDQYKVNVEKRILPFLEDEQKVTESFGTLINLLSWVDSYEQLLSRVGEQSESYVALRCRVKSYMPIFQDHVNNLIIDYSEKAIRKDKSEQQTLENITKLIRTKQDLLTYFPEDIFHFINQQLDILGPNLKGEIFIEFIRSVCGTLSEVLKKECRDFINELPSNEQEGEVSPLYLLVLQTNNYYKCMTYSNETKEYCLKFCNELISERVQQIFYQDLTGGFNEIINLLLEKSCKMVFNEINDQIIPFLFSPKWQNENLIDQALLTIKDYLQDIQLLMLNQIHFNKLVKLIFKQLINTYEEQLLYTVQFIAGIPKGFFPKLLTNLSFVNEQDKDKKKKEKDKKAAIQMILSNKELVVPQMQRDKDVILNSIEQDFIAQVGKTSAQQFDKILTTLIKSINNPKSEIDGLLQLYPQSFEQYSMYILEVVLWMREDCDSKYKQQKMEILNNLINKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XM34</accession>
    <name>A0A8S1XM34_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1280157</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000128">
        <property type="protein sequence ID" value="CAD8201718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XM34"/>
    <dbReference type="OrthoDB" id="10604394at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8201718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="276" mass="32898" checksum="BD9471346BDE035E" modified="2022-10-12" version="1">MSYFKKSKPFWILIILMIKYQFIKQLKYIKDQFKIIILFQEFHKNFYFAIQHADTKSTITPTSLQAELKQIIGVVQKMIIKSTNFDVQINQDVVFLDERLYQKKDAILDSLLVMKLLIDVIFLKIPKAIVSFQQDEEQIINVAEGLINLIEKIAMLVNVKAKVNGPQLIVNGQILKWQLSKITKVILNNQFDIDRDLLDGLIDLVQKEQIELNYNYFNPSQELQTKLQTFFNFTTLEIDEYKYKKTNLQYHLYKDRYIEYEDTIKQYMIDMFKTPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XMY7</accession>
    <name>A0A8S1XMY7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glutaredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1310033</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000131">
        <property type="protein sequence ID" value="CAD8202429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XMY7"/>
    <dbReference type="OrthoDB" id="283554at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8202429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="203" mass="24392" checksum="78BAABDDADF6A18D" modified="2022-10-12" version="1">MDQDLEIEKEINAMMIQKVWRLRKHIKAARQVVPSLREKFHFYYSSISFNMKATTSTQRLFTFVKNKKLQYFAFNLCDNPMCQQWVKQQRLNQDPLPFIFINKVYIGSMDELQMLIDFKLFDPIMNQEYLKHCLACMTPRESLENEKCQNCQTNYIFFQKIGQGVKICPKCREQQDEDKTICEKCQMDCIRVNKIFIHLKEVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XNR7</accession>
    <name>A0A8S1XNR7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Eukaryotic translation initiation factor 3 subunit L</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1310154</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000131">
        <property type="protein sequence ID" value="CAD8202657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XNR7"/>
    <dbReference type="OrthoDB" id="292728at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019382">
        <property type="entry name" value="eIF3l"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13242">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13242:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10255">
        <property type="entry name" value="Paf67"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8202657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="515" mass="61149" checksum="135999CB932EEA8C" modified="2022-10-12" version="1">MYEQELPSNLDDFPNYNLKQSAESEKIIVDNDQKKKAAYQFMQSLEKNLRTKISSPQQRIQTIAVSLELYDTIFNKIYCNTLYKETLFPLAETLEEAFNDEFDSITKLLFNESCYRHAFQSVQQQFNLQTSIESWQNYQLLFAALQQKQNIDLPLPPSWIWDILDEYVYQFYVSSRWRKLLKNDEINQLKNIQDFWNLEEMLKTLEAFYTQRNSSVQNTLQYLAFYSYLAAAKLHVMSGNFQVGYTLLSQIQHSELIIYSKSGGAYQSLFSYTGFCFLLNKEYKKANLTLTLIVNYFNKFKQLYTKSYQYDSLIKQHEKILALLAVTSLYYPEIEDNIITLLKESRNKLFEKYEKMLKYDLQVIGEILLQSCPKIINTIKNLKEFQTEQEVNPNEIIMDMKDKLLKEVLEAKKINEVEQVLSLYNQITFQHFNKLLPEKYIQLYIERNNVILNENSFESSVLSKFWQKSKLLDIEIQKDIIHVKEVQINQRDFINQLAQMNANLEKTFLELSRNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XQE9</accession>
    <name>A0A8S1XQE9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T1330162</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000133">
        <property type="protein sequence ID" value="CAD8203365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XQE9"/>
    <dbReference type="OrthoDB" id="10020333at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045216">
        <property type="entry name" value="CK2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054:SF0">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="45"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8203365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="371" mass="43226" checksum="72C231C70AFB396D" modified="2022-10-12" version="1">MKKQQVQAKAKAKTNDKQNKINQNSNDRYYPDLLIDEDAYLRNNYKVVRELGRGGYGVVYKGYAYHNGQEDKTKKYAIKVNFSTVSPELIFAEIGFLKLVQGKENLPQLVNLFLTNQKIYIVTDYFSFDPFITFFSTFNMQQIRDYLRELLKALLVLKQNGIYHRDVKPGNFLYNPKIKKGILIDYGLSEIDKSFVNNMLENGGNILELRQKKQLYEDIMKTISQIGHNKIGTESFMPLESILHYQEQSYEVDIWAVGVIFLQFLTRKYNLFSNVRMVHKPAVSKNLFYVNFILELTSLFGAEAITKICNKFGYYLNLPSVVAKSPINWRNVIHVEGFDDKAEDLLTQLLELDPQKRIKVEDALKHPFFEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XVD7</accession>
    <name>A0A8S1XVD7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ras-related protein Rab-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Small GTP-binding protein rab1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T1390090</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Protein transport. Probably involved in vesicular traffic from ER to Golgi.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000139">
        <property type="protein sequence ID" value="CAD8205007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XVD7"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01867">
        <property type="entry name" value="Rab8_Rab10_Rab13_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001018">
        <property type="entry name" value="Rab family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="188"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="200"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8205007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="210" mass="24432" checksum="DB0E7FA4E0913333" modified="2022-10-12" version="1">MNSNSDYHFLFKVLLIGNSGVGKSCMLMRYSENQFTNNFYNTIGVDFKTKTVAIGEHNVKLQIWDTAGQDRFRTITCSYYRGAQGIIIVYDITDRESFENVKTWIAEIDKYASESVNRMLVGNKADITERREVSYEEGLELSRQYQFPFYETSAKSSINIEAAFTHITKNILNREIHNSKAVVRKTSNMRLQTRQQQQQQEKKKQEDLCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XWY8</accession>
    <name>A0A8S1XWY8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1420121</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000142">
        <property type="protein sequence ID" value="CAD8206009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XWY8"/>
    <dbReference type="OrthoDB" id="1741717at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019341">
        <property type="entry name" value="Alpha/Gamma-adaptin-bd_p34"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14659">
        <property type="entry name" value="ALPHA- AND GAMMA-ADAPTIN-BINDING PROTEIN P34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14659:SF1">
        <property type="entry name" value="ALPHA- AND GAMMA-ADAPTIN-BINDING PROTEIN P34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10199">
        <property type="entry name" value="Adaptin_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8206009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="268" mass="31600" checksum="C1FA3C89D2F70644" modified="2022-10-12" version="1">MDQTNSNLIIIAPEHSYSIKLIQYLIKHFNNDELIFDKIDEEHQKIGTQLLINTKYYTCDVGLHTINYNYFNQVNALEQLQKIKVDGVIFIVDANNMNGLDQFINLCDQILNEIQPGLQAIIYNRKEGVKINFELLENEEKKLESFVEQIYLDLENPPIQQQIKQLSEQQDETLGFPRIIEIIECNTWTYMRPKSQEKKENINKTKVNQNKTQGLDQLEYEDDQFAQLIHDMTKIRTSKMTDEERREKAANLMNQLMNMCQDDDEDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XXP2</accession>
    <name>A0A8S1XXP2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TLC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1430045</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XXP2"/>
    <dbReference type="OrthoDB" id="284464at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042512">
        <property type="entry name" value="TLCD5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31898:SF1">
        <property type="entry name" value="TLC DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31898">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 136"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="16"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="7">
        <location>
            <begin position="43"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8206130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="262" mass="31111" checksum="7ECED1DCC97552E0" modified="2022-10-12" version="1">MYQWDLVNESSDQLRMFSYCFIVWSALFILCYDRIKLNNLSSKKQIDVFNRIVSILHGQFTFWGSLIIILSQTPYQLQEMNSSEMQFVMIVSAGYFAYDVIICTYFDLYDYWLIFHHVISLMAFGESILNKKYGHIIIFGMFITEISNLPMHLRHILGCFGLRQTKIYESIEILYFSLFIIFRGILSPVLLIRTYEDLHAPLLIKISASGLLVYSTYYIIEMVKITQRKIKSYRDRKRRNLTMYWFSQNPLLPSQKYVDSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XXR3</accession>
    <name>A0A8S1XXR3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1370055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000137">
        <property type="protein sequence ID" value="CAD8204414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XXR3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8204414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="107" mass="12973" checksum="14A0218508EC6D21" modified="2022-10-12" version="1">MDLESNDFLADGDNRNVKSRSKNNNNKRNNKVETLQRDQQKQKREEQQHKDEDWSDEEEKKSNAKKLEQQQKKLKQVQKQLEYKKDEEEMQPKLKGKEKRQAHVHDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XY09</accession>
    <name>A0A8S1XY09_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1440036</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000144">
        <property type="protein sequence ID" value="CAD8206443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XY09"/>
    <dbReference type="OrthoDB" id="2148418at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051210">
        <property type="entry name" value="Ub_ligase/GEF_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870:SF408">
        <property type="entry name" value="OS09G0560450 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="239"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8206443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="504" mass="59854" checksum="04A5F9D7FC990F63" modified="2022-10-12" version="1">MQKTKKYYQKIKNQIIFIQRQFKPIIVKSLSKHKEFLNFDKKETIQFNEFQKNNNKNLLDYEKLLNQQFDDQKIKIFQIILNLEFLVDLSDICQGWLQNYSLYWSKCFHKNKPIQFLEVSEAQTFVIDAVGKVYQWGLIDEILVKSNVKYFQVGENLAIYQTDKWNCLNLDEKQTKQNITSNYSWMYIKSNQIFGYKDNCIDSFIYSKNQAIRNCSLEFKRSIDQISCGHNFVLYLTKGQLWSVGSNKYGQLGLGDYNDRNSPCQTQIENINWIECGQTHAIFKSFKKCYGFGNNKYGQLGLSKKYYNSPQCLINPINENILSISASFRGTLFISESNKIFMTGQCNNFKSNKFIQYIDYPFLNKQHYIPIKIQCSWSKTISIINCRFIFENLYNVNTVKKNKSIHQFIQLYQNHSPNLVDPPKCEFVQQYCLQIYNKLQQHVDQKVTGKNYQEQKQVKFERKNIEQIKQQIIALQNKPKNKWNIDDHRFLETVNMLNILNQII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y0P8</accession>
    <name>A0A8S1Y0P8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1470024</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000147">
        <property type="protein sequence ID" value="CAD8207333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y0P8"/>
    <dbReference type="OrthoDB" id="304404at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8207333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="82" mass="9867" checksum="E70A23057BBBE2D2" modified="2022-10-12" version="1">MFLRIATTFFRQPLLKNQLRYTLLMQTPMLSILTNHKLLIQNLSYLQSQFYQMEECNDELELLKKFQQAECSETTLKHPQIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y0Q7</accession>
    <name>A0A8S1Y0Q7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1470029</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000147">
        <property type="protein sequence ID" value="CAD8207343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y0Q7"/>
    <dbReference type="OrthoDB" id="292086at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00043">
        <property type="entry name" value="CYCLIN_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8207343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="247" mass="28746" checksum="7AD768F7716FAFA6" modified="2022-10-12" version="1">MNNSINQRPPPPKFNWNIYDNSSSDDTKQQITLQKSDKRRQKQVILLIDEMLAKLTHSNLLNGINTENIKHQSIDLFDNSVIEKIKSKKYQSIACALIIQSLRILLIPLRIREITQTLNCDEKQVRKILIQLNQIRPFDQDAFTLQYMTRICVAIGFNQKFQTLCRFFYSTLKNQNLVQGEHEHVIASALVKLTGDFIFHEKGGLNLQIISNNAGCCEISLKNLLQKIEPHNQKLIQQAYEFYRRTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y2P2</accession>
    <name>A0A8S1Y2P2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cation-transporting P-type ATPase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1470066</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000147">
        <property type="protein sequence ID" value="CAD8207397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y2P2"/>
    <dbReference type="OrthoDB" id="301477at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF369">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="195"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="217"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="416"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="445"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="696"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="729"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="783"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="861"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="5">
        <location>
            <begin position="138"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="98"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8207397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="882" mass="103699" checksum="4A1B32E9449F30B4" modified="2022-10-12" version="1">MQNEINLINSPLLKHHREQEQKAQMIEMQVFKSLYQNDFDKHQKKDEILEIESNKLQQILEAKGQWKTLLKGWKKDGNKQQQNDDNYFGFQYNKLLEIFDLNQIIKEAQKNLTKQDQEKDLKEKRLKIQIDKLNELGGIYGLQNFLKTCIKQGLDDTNDYDLKMREKYFGKNEKPQIAIKSVLQIIIEQFQKERLFQLLLIITTINLIIYFDDNVQVYTFYIIMLVIMVLYKGVIKQQYNKEIVQHQNDIKERSKHLIKRCNVLRNGDDSIQIHNSKLVVGDILIFKQGDFIQCDGIITKLQNCNGIEVLQPFPNLFNTQEVKNIDEVKEQNVIHDEQIQPNLGLFDNFIFAGSQIIKGSGQLLVCSVGQKSSDYIQYKQSFLEDTKTEKQRTTYLKQNSMYNPFAFKIESFMNKIGIQCIISLLILLIILLFRNTYLIDITQILLYLSQLSIITIPSQLLQLINLFMIYVIKKCRQRSIYIQDYLSLYWLGQVNEMIIDKIDDKVTMFSKSQINIRYFTQLQLEDAQKIAIQTDLLKEDEVNDKCIEGSFLNSSFEQKNKFMFLSRRLVCPTLDHLKLERIQSSKTLEWLEQQKQKFQFQGIVDQQKLYEEIKNKNIIIGGNADRKEQLINLLKLYDKIIFIKGEENDGKAMEMAHVSIGPHESRSCIKLFNHLEQSDKSYISSMYESLSQGRAVYFYILSYSYALACTSQTIMIFAISSAILSDNIFIMPIPTFIIFYLTLNHFSYQIQKQCNQDVQTYVMHRSPYTKQGRIIDRHTMRKLYKLHSILIVELLIAYYYGYFKSVQDVYIGLLFSKHVFIMVRPIKQVLQQIEGIIYGLLINLINQFITQSNQDYQSISILLRNYSIGLIVVAFNLFVGYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y6W7</accession>
    <name>A0A8S1Y6W7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1520003</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000152">
        <property type="protein sequence ID" value="CAD8208727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y6W7"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histidine kinase" evidence="3">
        <location>
            <begin position="347"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="690"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="745"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8208727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="829" mass="96419" checksum="DFEF7BDAEA67E0FC" modified="2022-10-12" version="1">MVISTLNYQTLECLILIIIVLTHQVLTNYKEFGKYEKFTQNFYLKPFKQGQILQNLQNDKETPPPVQNYFKSLIRSQSKMTLVQSPKKCFTIQLKDLPDELKLKRYSNLTSFSKSQKNILSQGNSQFQMLQLYQNLINIFPYGILIINQHQSINYINNKCEKILECQGIEQVLEKVKMCVNTAKIQENVSETSNKQQKKQHHYQLLQQIIKKLQLNNTSFDVLDIILQPQKYFGILGQNEIQFYKDFQQSFHQQVFIYEWLIKSEQTSQNYQKKLKLIIMPTSMTNQQQEYISASSQIKSSVKSHISSHQSDQEHPVMLIIIKNVTNKYKCQQMRDEQIIHHSLIKSFSHELRTPLNSCYQMLNLLKYQSNSKASSDYIDIAQCSITLLIHQINDILDYAALQSFSFNYNITNFNINQITEEIEDLYKIQTQQKNINFTIKVSDYLLGRIFRNDKQRIIQLLVNLLNNAIKFTSEGGSISLNIIEAGQQYINFSVKDNGIGIDEHKLNQIQNCLQNTIEFGAVLKSHSKIKQPGLGLIIAAKLIEGLTESKDNKLTISSKKNKGTIVQFQIEDLQQKNLLSSHFPNQQSEKMNHLADQSEYNVQNVDQNKVVMSSNKTINLENDRDLQEVKSESYITLRLNDLEKQPEIYLPISPDYFKKKNLDQYNKIQKQLQSDYFKNEDLLCQKCVHILIVDDIPFNQIALKMILSKYQIEVDQAFDGFQAIEKVKQKMLKHCQNYKLIFMDIEMPGMDGFQASQQILELTSNQAFIVICSAYDTQENIQQGKNIGINTFLQKPVKQDQLNVLLGTVFKIESNSNFQLQQSKFHEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y7F9</accession>
    <name>A0A8S1Y7F9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1540125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000154">
        <property type="protein sequence ID" value="CAD8209441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y7F9"/>
    <dbReference type="OrthoDB" id="297993at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38150">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38150:SF1">
        <property type="entry name" value="PFU DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="504"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="558"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="513"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="560"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="574"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="615"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="637"/>
            <end position="657"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8209441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="763" mass="89712" checksum="DC025C76DBF5A216" modified="2022-10-12" version="1">MNSKQQHMTVCPALLKGDDQPDDFPLRAQNSNVPKVVVQKKIMIQPNNEILYERLNQEAEIFKQNNQMLKDYYEQEELRPCSFAPQTLNDGQEKRSLYQFLFDQQNHVLKIEQKLEQIKQNEMERDLQHPYHPKTNDFNFQSRDESIPTYERLYNLNQKKPSEPKMQQSESTVDFKPVIQQKSSNIVRNQKIEDILYQDAQRRQQKQKDAQDKKNAPKVVTVNVKYTSNNSEKIVAQKFIREFETVIDWIFDQTGQERPSNVSFSIDYLKLGEILQRLDFLNQLQAKENNEKAVEYENLRLSEERALLCEIWTVLRGDELGGISKRNLCLFLLTLIGITDFKIKELPSAQNEELPNYQKSIQPQQHKIVTAHQPNDRPKLGTIDKDGNIIFTFEETKKIQKQFDILYRNRLGCEELKKSNWKDENPYKPQILAQSKQLANQYREKLLEETANLIDNQLIKVNIPENGQITHADLLVLQKKAVDYHKEQKKQEILQQQLDKCPFKPQLLNNQNNDERKSTSKKQEKYLQLYSLAKPSTQKRDRTTEEIEYEKQLEECTFQPGLQTKNNQSIQQKQDNHYVNKDVDKTVQRMKQARQRREEVQGMLERGYKSNKGTQNQQQQVQSIQQKKDSRSESQNQIKQTSQSSRQKSMQSQDSQQMNTFQSQNFHMDESQPKEEQSIVRTGSQQDERIPLLFVDVNLGPSKTERIVVYEGDQSSDLAARFAHEHNLDEFMQEKLKELLDYQISGLLTKIDEEEGALSENDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8C3</accession>
    <name>A0A8S1Y8C3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1600094</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000160">
        <property type="protein sequence ID" value="CAD8210736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y8C3"/>
    <dbReference type="OrthoDB" id="2125762at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="236"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8210736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="708" mass="84611" checksum="06FC28761A87663C" modified="2022-10-12" version="1">MILMAATNTNTCNNDISAKEYFKIFQMNLETKFKQIQSINLEETANIIVSSQKLLELLNQLFYQDYYYVAISSFNNQDLFVDFQLKKKLQIIVNSYQFLQQRDKGNIKSSIEDSKMKVAISFIEKNYHYDYLTERFNNIQAGIAIFSLIQLDHKQFQNFFYSKRPSVQEQLFNQIMDIAEVSENLFLNLLLVGKCLSKLRQWEYALKVYAKLLYLIKNQQDKEKFRIYIYFKICKIQFLQGQINESKKKLKQLNNLIEQYENQQIEFYQPNNREIKHQSLIISLLISVYTNQIDLKREIMIKIAPIIKLINQSTQKRYFNMLQYEFFRLIQQQGMIFRSEEGQIQLDFILRKTQSVCQERQEIEQKSNNFFAIFKSIFDSPNQKGEKLLQIKSYFKAVVEFLIINVLLRKRDICILQYFNPNLYPLLRVFLEKNFLFNLEMKSEIYFFIHDFEEAYQLAKQTKNEKMIGQILEYWGKYEDAIKIYKNIKIPNIEISCAILLKILNDENRNKIEKCQSQQNSQENAITYFLNLVQKQGSYKIFAQKYLEILLQRSDSQVQLSEIIAKVEVEKTLKNRETGKYQQQEDVEEIVQTKSQDIETFRSVSQYLQEFSLEEDQLKQSFNFPIRKDIRLFYHRLRNDLKQEQDGCLINLLDEFVKKTQFLKCETEELFNNNDVVEPLGQGGNSKVNLLLQKRFNQSIWKITSIKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8F8</accession>
    <name>A0A8S1Y8F8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF8019 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1530109</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000153">
        <property type="protein sequence ID" value="CAD8209187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y8F8"/>
    <dbReference type="OrthoDB" id="347083at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058332">
        <property type="entry name" value="DUF8019"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42535:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_146, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42535">
        <property type="entry name" value="OOKINETE PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26058">
        <property type="entry name" value="DUF8019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13385">
        <property type="entry name" value="Laminin_G_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035822416" description="DUF8019 domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="DUF8019" evidence="2">
        <location>
            <begin position="262"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8209187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="336" mass="38863" checksum="352A0FEB66B96879" modified="2022-10-12" version="1">MFFIFYVFFLVVKSQDELAEGEEMESDCDMEFDMPFCSGNGTPMQENSFLNTLKILLSFNDMKVVNKIDQSEYEVEELERGPTLYDDGYSGVFKGSQILEMPQNEQTPYFTYSMWLFFPRTSGAERPRSVCPIFQKGNEETHYPSLYYDQKHKQFIVYIDQTVTSENTGLVSVSRPLANQWIYVSLLSTSSKLKLYINGVLDNIKIFQAAPKPNDYALYVGNTPWFKDYCSMMFLLDKFQYYTEEIKAYFIQAQAQFQLESASIHFGCISCNIEEASGACISGYHLCTKIEFYSGVYNVARQLGWMDYGDFVWTYQDAIRKNDKKKGLAVCCIDSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y935</accession>
    <name>A0A8S1Y935_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1570007</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000157">
        <property type="protein sequence ID" value="CAD8209903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y935"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8209903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="82" mass="9876" checksum="AC34E29B52776CDC" modified="2022-10-12" version="1">MEENSIRFQKYFQVISIDKDDSFIFVASDSSITLFQFKFTQFKLQKSQQLCHHFKQLLKQFLIHCRFNGFQNCIMVFESHGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YB59</accession>
    <name>A0A8S1YB59_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1510139</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000151">
        <property type="protein sequence ID" value="CAD8208694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YB59"/>
    <dbReference type="OrthoDB" id="21225at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="36"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="6">
        <location>
            <begin position="399"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="7">
        <location>
            <begin position="760"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="565"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="771"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="571"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="811"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8208694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="918" mass="107680" checksum="3C438D283D63FD3E" modified="2022-10-12" version="1">MLIWLRKQKRLALEIYEDYNDSIYIDCQNELIISQFWPLFVTIMKIYLFYSIVLIMISNIEEYESYNSQKVTLYTYLLILLPSAMIIYDRWIIEKLSKRQKVVLTQIIFITLETLLQLYLSQLIMEYESLQRLIGILLIFTIMLIVMATMNNFKLRLIILIKFFVYLGYKTEFTIFQYLFTYHIVFLNLLLVYYWDQQIMRLSKIKHFAEQSLRSIPTAVCILNSDCQQVLFSNRYMKKLIDKSHTKTKTFMTKSVKQDYLFETQPSIMSKYDEIFQFFSQMFANVEEVKNQLPPDSSKFNLDNLDDEQINLNQIIQYIIKTDKKTQGKIYTLRCQFIDRQEMINDESQKVVIEVKMKTLLQYEDNKHATLINLYDISTNLKSIYYKNLNQFKSKVIRSISHELRTRLNAIQGMIQMVQNYNQNFDREVQKYLKAAFNNCRIQNFIIGSIIDYNLLRERQLPVKLDKEVIDLFKDEAELKSIKIKYEDKIQTNNFELLDSEKFQSILIHIISNSVRFSKSDGLISIKLLKDLSNNNNNNNNNNNNNNQTNSYIQIPDNRAQINKHSKLQVPHSQSPQQQRLNGSECCHDSSNNKQRRTYSKYKMASQFSSNGNLSTNLADVYMIEVRDNGLGMTAERLEYIRSLLNGEDFQVERNSNDASSGMMLGLRASNQLIKNVSGKDENGNYISIDSDIDKGTIIQMHIKIKLISGENFSSELILKESSVENEGIVHSFQNFNLKLSPWSTVAQIHTKNLCQCENTFMIVDDEPYNLIVLESLLKKLQHQVVKAENEKMVEQFQDHKCKGYKGIIMDYQMPIMNGEEATKYITQICKDKNITPIPIFGLTGFSGEDEINRLLESGMREVYVKPITIKTLEDLISNISRSEYRSLDYVSNISQQFQFMDIEYAEEDKLVLEQSVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YCU3</accession>
    <name>A0A8S1YCU3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1670072</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000167">
        <property type="protein sequence ID" value="CAD8212296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YCU3"/>
    <dbReference type="OrthoDB" id="366390at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="155"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="394"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8212296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="602" mass="69281" checksum="8819DB6FCF23E514" modified="2022-10-12" version="1">MDPEKQLMDQPIEEESDMDEDEKREKGDWHQQLLSACQKGDITAVQRCLDKRANVLYEDKKKWNPLMWASCRGYTEIVRVLLSRQAAAPYVPEMALILQNFAQTMPVYSSPLNQSSPSKSTYFGGSPGGVAQSAVFNFPQQLTKKVVQGIINQQVRSTPLMWACFKGHVQVVWLLLKAGLSWEDVDQFGNNSVHLAASGGNQVVFQTLLFYGIAVDRPNTRGHTAKDLATNAYILQLIKLLEASGQQANYFCMTCRRFWKQEEYKMDWVFENAESTDMEKPEGRCLNCWNTIKKHTEELLSIIDKQDHKILTEKLNEIEKGIVIKNENGKEEFQRIEIDPKNYKFALIEQEKLRTQNVILDYLSTLKHVSNYKTILKSVNQIQAMLDDAVNRGVKIDAHVKEEAEKEMDRLKAERNLQFELDNLDIGLSTPEQVVVLNDKVVIATEKGVDKQYIDQASELRDKMAKAIQAKKILKMFNEYPAREIPDPIKWDPKTKKPIDPITGKPIDPLKLALQNQKKKKKKEPKFVIPEWANDVKSLDENIKGLEGLLKEAEVLNLNHEFVEESVQQIKRMKNEMRYRKEVDEQLKWIADAKAAEKKKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YGD4</accession>
    <name>A0A8S1YGD4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T1590084</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000159">
        <property type="protein sequence ID" value="CAD8210474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YGD4"/>
    <dbReference type="OrthoDB" id="449424at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001191">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="168"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="67"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="75"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="292"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="296"/>
            <end position="297"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="8">
        <location>
            <position position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8210474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="420" mass="49114" checksum="FCC0735EC66DB83C" modified="2022-10-12" version="1">MIQFDQQKQRTLQTNPEDYFNQDDDQPTETNMYQSLNYQKSHQPQNSQGFLVNQHSKLFDLRKKYLKNREDTNRRSVSIHNKMQCNKSPIKTQQTKRQSSPKQENYQPLQKKKTMPITKNLGSNLRNILKSNYQQQGSYGENPENVVIRKELSQLIEIPIEYQGIDSYSFIKTLGIGATAEVKMARNKELDIEVAIKIYDRKKMNNMHLKNLEREVEILNLIKHPNIINLYHTFENDKSIYLIMEYSSPINLETFMKGRPFKRINEDEAKILFRQIADAIAYIHTLNISHRDLKFENLLIDYNTRKVKIIDFGYSIKIDGKQSCSCGTPQYMAPELVKKSAYDQSVDVWACGVILFKILTGVFPFRGNSEKDLNRKICLGKLEYPSFVSSQARQLISSMLRVDPNDRICMQEVILSQWLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YMW8</accession>
    <name>A0A8S1YMW8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T2860001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000286">
        <property type="protein sequence ID" value="CAD8214988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YMW8"/>
    <dbReference type="OrthoDB" id="10043504at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="57"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="91"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8214988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="140" mass="16367" checksum="D2D3B8E8A26F43F7" modified="2022-10-12" version="1">MLNRYEDAIIWLDKVLVINPKDINSLFYKGSCLQMLGRYEDSIIWLDKALAINSKDVNSLFYKGTCLRKLKRFNDSLKYLDQALSINPNHAFSLGAKGQLLEDQQKYEEAVLLYEKSLKKQPDDQWTINRKAFCENKLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YS23</accession>
    <name>A0A8S1YS23_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EML-like second beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T2490001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000249">
        <property type="protein sequence ID" value="CAD8214774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YS23"/>
    <dbReference type="OrthoDB" id="414519at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002141">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="3"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="35"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="77"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="119"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8214774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="236" mass="25956" checksum="6C1B141D9311E35C" modified="2022-10-12" version="1">MSVCFSPDGNTLASSSSDQSIRLWDVKTGQQKAQLDGHDEVVMSVCFSPDGNTLASSSRDQSIRLWDVKTGQQKAKLDGHTSIVYSVCFSPDGNTLVSGSWDNSIRLWDVKTGQQKAKLDGHTSIVYSVCFSPDGNTLSSGSVDKSIRLWDVKTGQEIKSSDKNYKDILAQLKIPLQQNCPLQEASNYITTLLISQQAIFQAQGALILRGEFINQSGIDLKTLFKQKGSCFLEDYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YS91</accession>
    <name>A0A8S1YS91_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T2780002</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000278">
        <property type="protein sequence ID" value="CAD8214944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YS91"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035864114" evidence="1">
        <location>
            <begin position="18"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8214944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="102" mass="11789" checksum="521AFDC2E4E31DDD" modified="2022-10-12" version="1">MKQGLFILLALIPIISAFDKASAYQQCPQDQTECMQEVFGCVSQEQGCHNECGETEQCFQQCYRIGNKKFIKFNVQRILKQNGWIKYSEAKSMYDRIFLTAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RWX0</accession>
    <name>A0A8S1RWX0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0010363</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RWX0"/>
    <dbReference type="OrthoDB" id="294942at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8131942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="644" mass="75504" checksum="E0474B23D45027D6" modified="2022-10-12" version="1">MIRRDSGEMQQHAFSQPNFSKSTKDIQSSRKEYLFQKLPSLDSTQTNNFSQVKKGGSAKFIETDRVDTAQKPLTPQQQKKKINQTKQIEETTQFDLGSSRHFQRLFTIKNHTKSSTGSVKTQREDNTSIITNQIPQRKSNYHHTQQILQSQSQVQQQQCPFSSGKSIKDIISSFKDKKPKNGITRHSEFEKMEGLDDYTKELNSILNNDDLIGPILREVIEMKGVIKSIIDLSNGNKLTYHFETLKQLHSTLNITDQMFNRFKYLYLKYLIQLKINIETVFKCAQTVEFYRGAIVTQSRSIQNVKDNIRTIAKNMYSQIFEDFSLSPLFKGTKQEEQAIKFSRIFGFILGSAESTNFVMDSMRDFHKAFEITSVQYSIFKYYLSGSLSKHTQKEVVWYVLEQTDAYKAAVINQDSIKDLVYKQQGIDNFTSEFIKLCQQDQLINKNFIHKVGYDQFVEHSKYFLHYAFNKQNNCFTLDDLQSFHCQHSINHNLYQSIKEKMMILLSRLNPQRIIFQDFEDEYDLILPYITNQKLPCDIVGQQRLCEFVPILSDELKSNVEIKNIFDSNDVNVNKIVLKKFEYLLSGRKYFKRSDIQAIHSRLKISEHLFEKFVHLVEKAIRQYDTSLFWMVDAIKLWKYIIITV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RX23</accession>
    <name>A0A8S1RX23_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0010357</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RX23"/>
    <dbReference type="OrthoDB" id="442461at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34574:SF12">
        <property type="entry name" value="CALCIUM-BINDING EF HAND FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34574">
        <property type="entry name" value="CALCIUM-BINDING EF-HAND FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="12"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="53"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8131930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="95" mass="10962" checksum="0839632F608B5DE6" modified="2022-10-12" version="1">MSFRDILNNTQKVKKITKVAFNALDEDDSGYLERKELIQILNNCADTLKIERPSSEEMDEILKELDENSDGKVSENEFQKLIEQVLGIMAKIEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RXV7</accession>
    <name>A0A8S1RXV7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0010533</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RXV7"/>
    <dbReference type="OrthoDB" id="10255128at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8132282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="501" mass="59304" checksum="C2703BFD68A3EEC4" modified="2022-10-12" version="1">MLFEPDIMNRYQQFSTYQTQNVRVMEILNTALFERALLEGSYAQSLDQLAKILDLTEDGIINDDIIDIVQAYRSVILCEKEQAEYLKESYKNDALVILKDLLAKQQDGFSKMEYYCRQLDKQLKSNVNNLSRRANRYKQSIIDLPYAKTDSIRTALEQEKQQNSIFLRQAITDYNQTISEYFEGCAQQEQTVIQLDKQRRAVFNDIHMKLQVFHVSTIKNVEYALKKMNQKIESQELSKPIFMFKIEDQTRPVLNKFNINDYMMQTEMEIRNQQPSLNLKSKQATQEIKSLWSSIKRTINRDSSYDTVFNYLSQSNFVVNDQLFQKLAGSNEELDQFLDSLRESKLPIDAKSQLIKLNIYMQNLHKLLVDLIHYLYHRNLTEQTIKLIKQTLLKYQTFPVQDDLKDQITLYYDNILKQPCLMDVEFWKRHFIIKLHEQQQVDSQNEVKRHEIIAEIKFQSSKLLQHSINASVIIQAVQKLELEQDEIDQIINHIKMKVDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RZB3</accession>
    <name>A0A8S1RZB3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0010697</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RZB3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8132593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="98" mass="12148" checksum="9641269EF205736E" modified="2022-10-12" version="1">MQKYNGRSSLKTNDKLKKIHKNHKSFSNHTNMKQEHILLQQTNKRHLQYQNVDLELQNQKKDILKNRRKKIKTYKRSRRLVFKIRKQSQRAIYFEIRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RZN6</accession>
    <name>A0A8S1RZN6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0020123</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8132892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RZN6"/>
    <dbReference type="OrthoDB" id="295535at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="194"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="277"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8132892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="366" mass="42663" checksum="936C63732932617A" modified="2022-10-12" version="1">MYFYDYNPILIAQFSCIVMSLLSVVGVIIVFILGKGWRYFIVILFFTQIISMLFFYIPQVLTPFIFKDENSLSENSNNDFCQFKSDQDNRQYCIKETFCQLQGYFINSSFLASTLISLYSSYIIQQTLNPNSQIGKKVKQIYWIHFGIGIFTFAICLPMLFGAKYNHYGTSWGQELIYYVCNLQIGSDDIFTQIVFWLQTSITIVMIIIGIIFHCSVKKLKSKIRTKFVNEFDSCSSLNLYILPITLLIFWTINILQKLIDFKTDYIKESGYVIQTIWFFPQLLLALQGFNYASLFFYAFYLQLVPNLPKSLQSTYLFFARISFYNCIYGKISESKIRKDSLLFDADSAKDSTSSYIQKDDSSTNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S0N2</accession>
    <name>A0A8S1S0N2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeodomain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0010559</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S0N2"/>
    <dbReference type="OrthoDB" id="39591at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019185">
        <property type="term" value="C:snRNA-activating protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001006">
        <property type="term" value="F:RNA polymerase III type 3 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042796">
        <property type="term" value="P:snRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051575">
        <property type="entry name" value="Myb-like_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46621">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46621:SF1">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="114"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="174"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="174"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="483"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8132334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="536" mass="64361" checksum="FE85A40FEA2E858B" modified="2022-10-12" version="1">MANFLEFLNSLGQIKQMEIPFYYRNQFKIKENFINPNSKHFEQIAQKLLSEDNKGLKRKWDKQCKMLFIWVIVKYFQVRNKKTINPNLEEWQELSQIFNFDEVTLKQRWITLINPMTKSLNWESEEDNIIRSLMIQQEEKHIWTQIALELYNHNNGQYVRTPKQVRERWMNYLNPKLNKSSWTDQEDNQLLQIVVNSGKRWSLISTLLQGRTENQVKNRFKSLIHKIYREEDDDEIEELTAIRQYLSKQISDVINQDNPSLKSIKNEVKSEESQSNNINIQKLKKKQQIKNQDEKKQQIQIKKKKVKVIENLQIKNEIDQKLQSQIQSINQQFCLSTNGNCDTQSQENRKGSIQNQEFECKSQQNIQSQSKCSYQSLSYNGQTQMKETLPQYTGFRPYTNDICNDLFQQTPCQINRLGFGIKQQQYPFQQLSPFQQQIQQTPIVYTPLYLYNQQFMAQSPYKSPILSPHIWMQQQQQQQLIQQQQYEIEIQNEQLKQEQFNNRLEFLQGQDLVSKWKNKRVQEKQNQQELNKIIDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S9J7</accession>
    <name>A0A8S1S9J7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TIP41-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0050331</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TIP41 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S9J7"/>
    <dbReference type="OrthoDB" id="10253878at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051330">
        <property type="entry name" value="Phosphatase_reg/MetRdx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007303">
        <property type="entry name" value="TIP41-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021">
        <property type="entry name" value="GAF/PUTATIVE CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021:SF16">
        <property type="entry name" value="TIP41-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04176">
        <property type="entry name" value="TIP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8136758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="336" mass="39636" checksum="D092C0BEF93FC9DC" modified="2022-10-12" version="1">MNINQHKTYNDVNIEEYEQNGWNFIFTKAGMYNTSDLEMLQHALNLQGIPDITNGNNCGLIYNKQFNFGFLVDPKDALYLCNYQARDSNYIDIKKELKSNKLNYISMIPEQAQIKYANIWNQKQNADIKILNKISDTFYSSPYKGTIVDGDQISYQFQRFKQISGIKGKSIFEQQLNENLIKNEVDYLNQVYNTNSKTFHCECTEDEIPLNNLTPENPIKWAAHISLFEDELGDNGLSNCEYRFRIMGDCFFGLIRNYLRIDDVLVRILDTRIYHQFNWNYILRDFTHREDSWANIQKNGFNFTPQWLTDQNQSFLIQNSVPIQLHINDKIYFKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SBX8</accession>
    <name>A0A8S1SBX8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">E2 ubiquitin-conjugating enzyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0050252</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SBX8"/>
    <dbReference type="OrthoDB" id="7851174at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23800">
        <property type="entry name" value="UBCc_UBE2K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000088">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="5">
        <location>
            <begin position="4"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="4">
        <location>
            <begin position="157"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="1">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8136642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="197" mass="22465" checksum="983954239C969643" modified="2022-10-12" version="1">MQKTQTNRLNKELQDFNERQKKGEDSGISILLVDQNITHWKGFINGPSDTPYANGYFQVDIVIPQEYPYKPPKMKFDTRIWHPNISSQTGAICLDILKDEWSPALSIRTALLSLQALLCDPQPDSPQDAVVANQFKTQKDLYVKTAKEWTQNYASKNKQEEKVQNLVNLGFEVGKVREALLRFGYDEEQAANFLLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SET3</accession>
    <name>A0A8S1SET3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase M16 middle/third domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0070056</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SET3"/>
    <dbReference type="OrthoDB" id="301823at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050626">
        <property type="entry name" value="Peptidase_M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032632">
        <property type="entry name" value="Peptidase_M16_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690:SF18">
        <property type="entry name" value="INSULIN-DEGRADING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690">
        <property type="entry name" value="NARDILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16187">
        <property type="entry name" value="Peptidase_M16_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="26"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 middle/third" evidence="4">
        <location>
            <begin position="553"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8138320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="960" mass="112305" checksum="1B913A252E5B76C6" modified="2022-10-12" version="1">MQMDEENQGLVHQDVKKPKDPETRGILSTICYIIQLIILILLVFLVFKYALANHKQSSQIPQIQQLKVNETENIDDIDDTVVLDKEDQDIMQFLTSPEDVTQFDNIIPEPEEEQFEELIFEAEPYNEGQLQGWQFKNGLTLIVQEKQVEIATLQFVIYHGYHDDDYNTLFMHLLLKQQCSQAQLIERAYMSTITTHFKNELMETCIRKFADSLVREYTQDDLDNINSEQIQTNDIDLIFKIDRVLDAISLNNEIMTEPMQIQDLQNYIELILVSNPLRLTIESALSNQEIYMLVSDTQLLQKEFGTSIIDSWDLEPILYNQIRINSNQTSTILIYPAKQDEFFKHLLLQKTQNSLQTILKCQFDILDFDFLAINIQECDVDSITLANAIYNFGSFLKRQNEKTLLNCHKDFYNLQILYQKYIEDKEFSKKDLNAIIDNIQSGVIIIQQGQFIYQELKTLNKEDFLNSNFVNKKIQSIEYSNILLDYTIVSNIDEQLISQLKLPNLSQYIPLELPNYNFYTTNEIVPINITLQEPQLMNDSPNDIEPVYKPLTPYIPLDLDQSQKNYLIPILTPLQISQELWWQYDNHTNRVFLGILFYKPINGYSIAKSKAILKIAQMYIEQSSLDAISAGFNFQVESSYKGLSFMLSGWSLTFEAVKNQVLSNIKEIKDESKFTIIKEDLRNQFQEYYFSKTQFYLVQYWLPKLMLRPVNDPADIIREFQDLKFNEFSQFLTDMFSDIKYTAFISGNIDIQNAKQLVQEYHPNDIETITKTLQINKNLIHIIKLPSKQKQNAVVKYFSVGKRDFLTTAALLLLQLHFESQQTIQVSFKSIGCVDGLLFYNQGIDSVIKLNEQIQQLIEQQKYDLNNLKDRLILHYSNNDEFLWDKIYNRNYAFNEINEIKNQIENVQISDFQGILQSETLSIFGVTESSKIPNIPSGQNDYSKQKEKSYFQCAYQYQSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SFQ9</accession>
    <name>A0A8S1SFQ9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0070283</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SFQ9"/>
    <dbReference type="OrthoDB" id="285351at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8138728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="91" mass="10688" checksum="099CB0F3F26F768F" modified="2022-10-12" version="1">MINDLQIIADSQNLYDLYPTNNSCPVGELMEEDLQNKRHYISSLCFQLKKKIKRQQNRPSILKIEDSGRNVKIYQNTPSSNNLERMENETQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SMV4</accession>
    <name>A0A8S1SMV4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0090285</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SMV4"/>
    <dbReference type="OrthoDB" id="302705at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042392">
        <property type="term" value="F:sphingosine-1-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969:SF13">
        <property type="entry name" value="AT30094P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="2">
        <location>
            <begin position="104"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8140796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="202" mass="23208" checksum="726B5AAB6E448027" modified="2022-10-12" version="1">MRQPPFEHCLDCPIIIKLMDACPLSPFWQIVQEEGFSGLADTTLLYELFMVIISLVPWISGLILGILLLKRRDMRSLIRAGTHLCAHITCDVVKVVYFREDRPDGSCSLKYGLPSCHSIFAGLYTSWVLIEILVIGMKLKTRHIIAVISMLIVPYSRIYLIYHTLKQCIYGWTIGFVFAVMGLTLHHQCKKRRKNKQVKVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SSK5</accession>
    <name>A0A8S1SSK5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0110002</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SSK5"/>
    <dbReference type="OrthoDB" id="282438at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="499"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="501"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8142187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="635" mass="73696" checksum="472B2FB2BDB31B49" modified="2022-10-12" version="1">MVIESIFHTNVKNKFKYSASNLNVLKVIIMLSQILNLAKASTIIYENFFSSTSINNLNSFYYNNNCDSVISTCGSQTILKVNQCSPNDYDFLIYQTIELNPHYLLFLSFKFWRIDQWDNKYFTVYIDNKLIHGAVYSHTSSPSDLCGSTSYNDEYYSISGIIDHSSTTVFIVMLAQRGIWGISELEIQIEQCPTGCYSCNKDQFLDEYLYFQQFVSKTISSISSSEGWIKDGMVQTSVNGCLDWKYGNVNSGKNLMKVLKLDQHTAISFQLKVLIFNSNPINVFIKIDDVIVFQTEYSNGWININHAEELCVFMQMKNIKIHQYTHINQQIQISVLLNENSKFGIRDFQLFLGTSYFQNMCADYNLLAFDSCFSNIYDCVEGCSNCIKGECWDCQEGWEYNRYLRICIPICGDSKIINYEQCDDGNQTPNDGCHLCIYSCIKNFFLCQFGICYLCNITSRLSVDQQIFIRIDDDQDVKNFIQTQNICYHIYQQMIEQSSETVEESKEEEEEERKRKRKKKEDENLTIECMSYCLYCEDQHSCLHYGVIIQGYEDCEDGNNDPYDNINVNFNVHLDAQIVNKVIFVKNVIIQKFEIITILINLLTRIIQNQLVFQEQKIKFQVGDQCGNGILNKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1STH8</accession>
    <name>A0A8S1STH8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0100314</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1STH8"/>
    <dbReference type="OrthoDB" id="307436at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8141802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="245" mass="28285" checksum="CDFD4A09B48217FB" modified="2022-10-12" version="1">MGVVQSCIKQRNSAPQIEYNVPTILKRKQIGKHPHKQNILIPPLSQLGEYHESLEQLELALQENNINFSIHKTATFNSSSANRQSISINPSLNLSLNTTIPNMLQPQINQNLQFQEQPQHKDEQIIQKKQTYKDSQFKIRAEVQIDKKDSYARSLSSNSRAQKAQNPNNDESSISLKRDSETNTKKSILKNKKQIITNQLNRGVSLRQQQQVINDDTQSQRTSKSQKRVKFDPKIFRPKNTHFFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1STY4</accession>
    <name>A0A8S1STY4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0100410</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1STY4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000445">
        <property type="term" value="C:THO complex part of transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008501">
        <property type="entry name" value="THOC7/Mft1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05615">
        <property type="entry name" value="THOC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="139"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8141992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="191" mass="22646" checksum="4EEC1D287D4B6032" modified="2022-10-12" version="1">MNRDLQPPYKLDPDSTERIIKDLLIYKDAQLKVILKKYIEITAPQETLSEEKLQQLAAEFQKEIAVYEFQIAKATRQLEAQNFDREQNQELNKSIIEGIKQTDLEIENLKSVYQIEEQIKINRQHYENLSKQISSYENVNNVVKNIEVATKNIEQLKIQHKQAAELIKIKEKQLFMMLHLMQDLIEVKYEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SVH9</accession>
    <name>A0A8S1SVH9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0110290</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SVH9"/>
    <dbReference type="OrthoDB" id="287389at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8142694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="220" mass="25860" checksum="7A90EC5DB5833FA3" modified="2022-10-12" version="1">MKNESESRVSLDNLSINKATGPLVTNYKQLVCCFVKQMLFDVVIPGQKMFTEDKKQNLRNQILFYQQGFARNMNDEQAYSELIMLTTSSPHFIELHKRTCYNLIGQNQEIEAQVNKELNRSISSIETFCGNIKLIDKNIIVNWSTNRQKLYSKTIIDIIMRGLLLSYILEIIIRSSLKQDFTFNFAITIEYNQCCLEKEIQPIFKINDQTTQYEFKFYKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SW62</accession>
    <name>A0A8S1SW62_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0130169</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17919">
        <property type="entry name" value="DEXHc_Snf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623:SF17">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="10">
        <location>
            <begin position="73"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="9">
        <location>
            <begin position="283"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="410"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="719"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="37"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1001"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="53"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1001"/>
            <end position="1014"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8144320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1292" mass="154087" checksum="36C5506A6005702D" modified="2022-10-12" version="1">MRNNNQRENQEKRVRKPSRYKFQDEEEIDQIIKSCEKSSKSRKLRKLSQESSMEVEEEEDEQSDSQYESDYNEDYCWKCRQKNRPLLCCDSCYRSFHMACVGIKKMPAGSWYCPQCCQYEQSYCPYCDEQSTNEKIICSKCNTFIHLECILKDIPFEAVLRSPVNRKHISEELFQQLLSAEKPTKTAPLIFHCCLRCLQSFGVGQILAHLKFKDKQCYLILLEQSSYLHCHFFSQTQVQTVNQRKLKNYIEKNEGKQDAIENDNDAELQTSLNYIEEDILEYLEPERIIGCKRGANDQKKTWREIYNQEMPKITQYGTKNNYKFLIKWSRMFYEESSWEDAYFIMEKYRDEFKRFLKSKILEETYGTPEYDQFMKQFLKVPSSKFKKNQPAWITGGQLHQFQLQGLQWLQKSYETNNHVILADEMGLGKTIQTISFLNFLQYEHKKSGPFLIIGPATILYNWLKELKKWAETFNVIVYTGNQESRDIIKAKEFYYSNNNNVCKFNVLITSYDIAIIDQAIIKKINWECLIVDEAHRLKNNDSKFFKVCSQFSSQHIILLTGTPLQNNLQELINLIEFIAPQKVKQLKKEQLNLLFNNQDLEDFQEVKKTTLTELNSLLKPHILRRTKADVKLQVPEMEEIIIKLCLTDKQKFLYKNVMLRNYEKLKVLDQKKGASKANLLNILMSLRLVCNHPYLFTYKREFPNEDIEEMINQSNKLKFVDRIIPRLLEMQHKMLIFSQFTMMLDLLQHYLQLRGYSYERLDGTTSIMDRQRIIDSFNNSSGKSKIFLLSTRAGGLGINLTSADTIIFTDSDFNPYRDLQAISRAHRMGQTNKVKVFRLVSKYTAEERIIQIATKKLLLEEIIINPINKFSKDDFQSIFKESTWELFNKNLEEKDQEFTDEQLNILLQRDIELQSDQQYVNLKKNDINDYYLSGFKFTSFNLEQVEKEGDESHQNVNLDKYWETFLDEEAKGFAEKEKDEFGKGKRLKKQNIPNYHEDYHQFSSSQYSPSDQSQGTGSEQENDENKKDQKIVSERMQKLIDKDLQIMRFFQQTVLEHSELAQLSVDNDIVTYGFTAYHRLEFLDFILSFGMDFDSIDQFYQILQREKPQCFSAEYKPSNDEFKKYVKEFYSLLLDYEQFKDKRNIAFCNINPKDIFYRICGLMYLKKKYQFYQNRQAKFTIKHENYQKNKVQKDQEQIIYKNEEWTNFDDFTLCKYIAEKGFNAIDQIMEDQGYFQFASELQEYPFWFKLFRKIDQVPFEQIQNEEQVKKYLKYWIQQRCLMLMSLIIDSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T015</accession>
    <name>A0A8S1T015_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0130166</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="68483at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001443">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF77">
        <property type="entry name" value="SERINE THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8144314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="325" mass="37791" checksum="8D53BFA680569F00" modified="2022-10-12" version="1">MTLRYSSDLTLNIYEKPPQNPDWLQKQGNIDLILNKIKQKNMLQRIQYTXFEVIDDPNKDKLYLVMEYMGKGSILSKGFFKKQKTTSNILDEIEDKNIVSKLTEEQCRHYFSDFIKGLYYLHECVNVIHRDIKPENLLVNINNQLKIADFGVSHIMEDGGDGRISNQTGTQAYLAPEVFKGQNFDGKPVDIWAGGVTLYQMVYGKLPFTSQKSMELRQQILEENPLFPQPQGFSSSIIKLLQGLLQKLPEKRLKIDQIIMDDWITDFGKQPIINQYIEYVDVTEMDIRFALTSLNIQMALKIVVKLLYQAKKARRKIAERKKMQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T028</accession>
    <name>A0A8S1T028_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATP synthase subunit beta</fullName>
            <ecNumber evidence="14">7.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPENT_87.1.T0150442</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O + 4 H(+)(in) = ADP + phosphate + 5 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) and CF(0) have multiple subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="14"/>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T028"/>
    <dbReference type="OrthoDB" id="14523at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042776">
        <property type="term" value="P:proton motive force-driven mitochondrial ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18110">
        <property type="entry name" value="ATP-synt_F1_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18115">
        <property type="entry name" value="ATP-synt_F1_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01133">
        <property type="entry name" value="F1-ATPase_beta_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1140.10:FF:000005">
        <property type="entry name" value="ATP synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.170:FF:000005">
        <property type="entry name" value="ATP synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000026">
        <property type="entry name" value="ATP synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01347">
        <property type="entry name" value="ATP_synth_beta_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005722">
        <property type="entry name" value="ATP_synth_F1_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050053">
        <property type="entry name" value="ATPase_alpha/beta_chains"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01039">
        <property type="entry name" value="atpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15184">
        <property type="entry name" value="ATP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15184:SF82">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039072">
        <property type="entry name" value="ATPase_subunit_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 14" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 14" id="KW-0139">CF(1)</keyword>
    <keyword evidence="7" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="169"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8146625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="499" mass="53613" checksum="223E1AF0832E3507" modified="2022-10-12" version="1">MLTRALAKQMRLGFSAAKKQAETGKAATTTTGQIAQVIGAVVDVQFDGPLPPILNALQVQGTSHKLILEVAQHLGDSRVRTIAMDSTEGLIRGQPVSDLGGPITIPVGPETLGRIMNVIGDPIDERGPIETKIRYPIHREAPSFVDQGSGAEILITGIKVVDLLAPYARGGKIGLFGGAGVGKTVLIQELINNVAKAHGGYSVFAGVGERTREGNDLYHEMVASGVINLSGGSRCALIYGQMNEPPGARARVGLTGLTVAEYFRDEEGKDVLLFIDNIFRFTQACSEVSALLGRIPSAVGYQPTLATDLGQLQERITTTKKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRALTELGIYPAVDPLDSSSRMMDPNIVGEKHYTVTRGVQKLLQDYKSLQDIIAILGMDELSEDDKLTVSRARKVQRFLSQPFFMSEVFSGRKGKFVSLSDTIAGFRALLDGEGDEYPENAFYMQGTFEDVIEEAKRVAAETTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T3S3</accession>
    <name>A0A8S1T3S3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0150213</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T3S3"/>
    <dbReference type="OrthoDB" id="2204368at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080132">
        <property type="term" value="F:fatty acid 2-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014430">
        <property type="entry name" value="Scs7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12863:SF1">
        <property type="entry name" value="FATTY ACID 2-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12863">
        <property type="entry name" value="FATTY ACID HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="8">
        <location>
            <begin position="18"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8146176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="154" mass="18033" checksum="EA720B233C67773F" modified="2022-10-12" version="1">MQFNPIISPIYFDDISAVQTYARQNHLLLYVYKNTIYDLSEFAYTHPGGLQEICIYANTDISKIIFNPQIHKHSPLILFKMSKFTFGYIQPQKQKTLLKSKSSQQLLQQTRKNLLSATDPDYLAVPEDCCIEQVRSIKTHRRIYHSYEQISQPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T4E3</accession>
    <name>A0A8S1T4E3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0140391</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T4E3"/>
    <dbReference type="OrthoDB" id="310721at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="186"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="220"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="254"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="288"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="322"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="356"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="390"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="424"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="458"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="492"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="526"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="560"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="594"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="628"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="662"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="696"/>
            <end position="729"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8145714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="773" mass="89874" checksum="922E901C9666B53E" modified="2022-10-12" version="1">MEIFKCQYIGHENEEIIGFCLNQNCQKVTQYCYECLNTTHSEHFNDCIRFTKMNQFMNEFIQVYNQQMKQFKEISQQLQNCFEQIFKKMDQEIKTLENITQQLLNKDYLTFKQQIHKIKQYYSKEKENQIQQQIIQLNNIFDTIKNIVPEQNKVDDKFYEVNQNDNKIQQNNKEIDEKEQEQIKVVQIKFNEGVQLHNLKKYGEAIECYDKAILINPNYADFWYNKGIALKNLKKYGEAIECYDKAISINPNHDIAWYNKGIALKNLKKYGEAIECYDKAISINPNHDIAWYNKGIALKNLKKYGEAIECYDKAISINPNHDIAWYNKGIALKNLKKYGEAIECYDKAISINPNHDIAWYNKGIALKNLKKYGEAIECYDKAISINPNHDIAWYNKGIALKNLKKYGEAIECYDKAILINPNYADFWYNKGIALKNLKKYGEAIECYDKAILINPNYADVWYNKGIALKNLKKYGEAIECYDKAISINPNHDIAWNNKGIALYDLNKYGEAIECYDKAISINPNYAVFWNNKGIALKNLTKYGVAIECYDKAISINPNHDNTWYNKGIALKNLTKYGEAIECYDKAISINPNHDNTWYNKGNALKNLNKYGEAIECYDKTISINPNHDIAWNNKGIALYDLKKYGEAIECYDKAISINPKDADFWNNKGNALKNLTKYGEAIECYDKAISINPNYDNAHSNKGFALHQLKKYQEAILSYDSALSMNINHLRLKLKADSLFELGNKQEAKQFYLAALQQGSKEKDYIQKQLSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T5A5</accession>
    <name>A0A8S1T5A5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0160364</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T5A5"/>
    <dbReference type="OrthoDB" id="292971at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8147403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="455" mass="54130" checksum="1EE31B68FEF698FE" modified="2022-10-12" version="1">MGNYCHSEETPKSEKPTLRDSNKGKLVFQNLILTNTQFIEQCKDSYFIQFHKNIVEKDIESLSKFMNQKMLDSSIKGSYPTEEEKQIISKNISKDIQQREKHLYNQLIFHYNQFLNELFELDQNLQLITKSNYNAGDVRVNAQLIRNTFVKRSLSDNHRFQEQLLLDENIPHNTYIDQNLSRQFRENKQLANLFLDQLSCIFKLCYLILEYVYSNYILSIFGTSIDSFTDRYLLISKIYQKNIIELNPDIQKLITAAIQAKYEKKSSLIKSEHMSPAILQPQLIPQTVDSIISHESEIQLLDSIEKNLKYQQQDQQIQNTQQNFYYSQTFSTIDKIINCKMPWLKFKLIGKLDQQIVDIFLQYRSQSQIAHFIDVIKPEDSKVEVLLYILQRYMSSRKNLNLPLCYYELRWMQEDDNVLHKKVKTITSPYFSRFLSLYEISFKNYQRKQAQLLYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TD46</accession>
    <name>A0A8S1TD46_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0220279</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8152101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TD46"/>
    <dbReference type="OrthoDB" id="304357at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8152101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="185" mass="21254" checksum="E4CC4F70B5DD32E7" modified="2022-10-12" version="1">MQYKQEFGISLGQLEEDLNCDETSPSRQFDSFQLINQLSIKTKDSVEQKFKQIKRRLNHHKSDIHRPAQLLNVDQKNNQTEKRGATYERLPSDLQIDSQIRQAKKKNIKVSEQCLSRNNVLESNSSSYSSKLGSKQNCVKGILKKQTLDSSFSDNNSRCSSIGSKKSVKFNLSKPEVRMALNQWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TED8</accession>
    <name>A0A8S1TED8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0200002</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8149924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TED8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8149924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="97" mass="11656" checksum="21D385E89DD8AD70" modified="2022-10-12" version="1">MQQLFNNLKILNKHYQPMHRLNLLHNQVNYLSDCNLVYCIHHSTIYNNLIINIIHQENQNINIYYQAGAEQIHGKHTPVEFVFQIQQCVWKVRNQQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TEX2</accession>
    <name>A0A8S1TEX2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0200265</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TEX2"/>
    <dbReference type="OrthoDB" id="302782at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="53"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="147"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8150448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="292" mass="35550" checksum="340FEE77A800F947" modified="2022-10-12" version="1">MFSARDQLSISRKTMQHYSIASQKPIRYATQYCGDTEYSTSIFNSDYELIRTKEKYKQKCEEIDMKKNQLNSDHLDLEFRAEKLRQGENEVKYMAELLKKKLKQIKQQEQQIKENQISFADKFREKELSLIEQQKLIQEKQDQLILREEQLKIREQALKEKESLLQEQITYYTQFNDRLKLNLEELNKKEQFYIQQISKMNQTVETLLYHENHIRDLEFRLTQQIQGLKDFDNQIRIQVQNCKQQEDLIDQKEQGILKSQELINRKLLWDQKNITRQHLQQISNYMSDQKYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TFS0</accession>
    <name>A0A8S1TFS0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Carbonic anhydrase</fullName>
            <ecNumber evidence="2 10">4.2.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 10">Carbonate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0200378</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the beta-class carbonic anhydrase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="2 10"/>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TFS0"/>
    <dbReference type="OrthoDB" id="10248475at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015976">
        <property type="term" value="P:carbon utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00883">
        <property type="entry name" value="beta_CA_cladeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1050.10:FF:000001">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001765">
        <property type="entry name" value="Carbonic_anhydrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015892">
        <property type="entry name" value="Carbonic_anhydrase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11002">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11002:SF76">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00484">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00947">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00705">
        <property type="entry name" value="PROK_CO2_ANHYDRASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9 10" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="268"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601765-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8150627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="288" mass="33275" checksum="9FE2CCFAC2E28AFE" modified="2022-10-12" version="1">MNQQIVSNSIQEITHYNRVLEGNKRYVAKKLSTDEQYFKKLSQGQSPKYLLIGCSDSRAPPNELTETDPGEIFIHRNIANLVIPTDLNLNCVIQYAVEHLNIHSIIVMGHTCCGGIKAAMTQESVGGLLDLWLNQIKIVYEKHQDLIEQFDNQDDKVNCLSQLNVRAQVMNIWKNPIIQKSWQKGNPVMVHGWLFRVETGYIEELLIDSQTPEEMSKVYALKFKLDSERILQKQASIQSSPRNNARKRFQLMQKKIIQNLKNLKENSKIEQDIDPEQLREMVQDSQEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TJQ6</accession>
    <name>A0A8S1TJQ6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0210231</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TJQ6"/>
    <dbReference type="OrthoDB" id="292614at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8151219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="214" mass="25536" checksum="222977ABAD08F2F9" modified="2022-10-12" version="1">MKRQILNEHLQMKKNRSCQISKSFQNEFSLERKQSISQNKKNPDKLGSMQIVEMWSKKMKKQCDSYLEVLKHKQEQEIKKLNHVPPIMLKNIDVMKRSDKTTLPRSRITSFNQHDFNQRSIKEVIQKNDEQIGTSILSISNSGRSMAQPFFFQRLKLSSIREVKNCIDQCDTLLQDVQSISKEFYQPKPDRFKLYVQKRQKEKLKELFLLKRLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TKI1</accession>
    <name>A0A8S1TKI1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0220043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8151666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TKI1"/>
    <dbReference type="OrthoDB" id="302341at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="36"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8151666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="133" mass="16066" checksum="24BFA48EE4689E38" modified="2022-10-12" version="1">MTSELSEDSSFYDLKFPRSLTTSVRKQGQEDYFFEMKEKQKQIEDLTYQVKLMAANNQTLNLQNQMQKSEIERLLRFINEQQNEMKKMAQRDNKKQINTRSIKMQLINKMCLWAKQFPLDQQIQFEQIVQMII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TQR9</accession>
    <name>A0A8S1TQR9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0250053</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8153832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TQR9"/>
    <dbReference type="OrthoDB" id="289564at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8153832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="214" mass="25643" checksum="B956D9FA7330DB55" modified="2022-10-12" version="1">MSNNNYIERHKRILNIVSHLGLNKKKSSIPAFEKKRLSQSRIIFYKPEQCQEEEEDQLILKAKEVLSKPVKRKLIRSESQLVQMISDKYDMLKDDPEENSAYHHIQLSLMKLQEQEILGQFHEVKQNLRMSKTSYPDPITIPQQKVQIQNMFEKKCSQHTVESPQENTIRQKINDFLYKTRESFKDFQADVSNYKNLKQHHRLYKQRTATYFQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TVB1</accession>
    <name>A0A8S1TVB1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0270114</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TVB1"/>
    <dbReference type="OrthoDB" id="10252009at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000128">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF511">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="6">
        <location>
            <begin position="42"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="7">
        <location>
            <begin position="105"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="144"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8155482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="245" mass="28503" checksum="17A8669F2C0F3D8D" modified="2022-10-12" version="1">MSLTSDAFSRSIVLTTIPLEELKFVYSVPQIVDPDPITCIIEPTHEEGGIYVGGYEGAKDLEMLKRLKIRAVLTASQETAVQYSDLVVQFHHVVQAHDKDDYNILQFADQTFDFIERHRKHTNILVHCFLGISRSPTIVVAYLMKKYNLNIEKALWKLKSKRRQVNPNTGFLKQLLNYEKLLQQQQQQQQQQQLQQQKPKGIFKMVRQQAIPQTQMLPQSRILPMDSRVIIQKSPSQYLGQTYYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TY17</accession>
    <name>A0A8S1TY17_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0290021</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TY17"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8156492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="169" mass="20253" checksum="1290B2C8D6D61444" modified="2022-10-12" version="1">MEYLIYGKIKSCNRLNNAIKLKFKKKDKQMNRRPRCTHLNSSNYDKDRSTIYSLLSSMNERLIYETKKILIKIEKYERVINFFKNFVRNHFQRKILITRQEGSHNFFNPNLKREKQGGHSISAWTEILQLRLGRINKGLDDKKFKLEEHSGTVQLYASLSQLYYISIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U1B7</accession>
    <name>A0A8S1U1B7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0310206</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U1B7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8158174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="258" mass="31208" checksum="514C7BC0DB84BBC4" modified="2022-10-12" version="1">MIDAKYYLQKSQLIMKHLNSQDQNVVEWIIQLDETQKQFEENQFHYPLLEQSVQFINHIVIHPFLQLLEKKGLQFLKFFNMNYNYNMNNNLLYKITNYRLNMYKKTLDNIAFILQLKKRIYQISQKFQWIFQKVQVKKKIPIKNGIKYMGGAFFITNIDKARQIQLKEITTFCIINLNFMEDLKQGSILKKLYQILNDQNLISQQNIYSLCIQAYITLVQINQINNEIERGTLFQQEQFKRSDIRKFIQKIDIISIYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U1T0</accession>
    <name>A0A8S1U1T0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0330032</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U1T0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001434">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="256"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="301"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="92"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8159245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="519" mass="61272" checksum="AA0DE20BCED7B015" modified="2022-10-12" version="1">MKPLMECDQKLSDQFSFIRISDKIVKTINYIKQLSNQRFHKQQEQQCSHQQRNQLPQVSLSDIQDEQLNNQFVDKITFLMQSSIENQVKFNKECLNQQLEMQSAQYQLQNEKEKIKTKKLEEKIKAKDLQLEQKNKEIQSQQKLINELKNQSIKNIKEKKKLEETIRAKDLQLQSQNLQIQQLKQIQTQQPIIQSSSQLILKPFTYNIIHNNSIKQNDWCYAIAINKDCSIVLAGCNSEIKVFEFKQEVLKKTQLLIEHQDDVNTLNFMNKSNHFISGSDDKSIIIWSMNQRNQWICQQKLNGHNNSIYCLVLNNNTEDLIISGSRDKTIKFWKKQNQWLCQQTITDHTNNVLGLSLNQQQNKVISCGDDKQILIIEQSQQDSKWIVIQKIMVETYGYRICFINDNLFTFQPFGIEQMHVYEMNNNNKQYLKTKDILGKGGYDSFLFPQQHIKSKYLLVNKIGKYVNLIRTKDNCEFKAEQYIEYKSSAIYGCMSDDAQYLITWDNGSYEIQIRKYNEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U360</accession>
    <name>A0A8S1U360_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0310341</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MCM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10251574at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000003">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000217">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF66">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="12">
        <location>
            <begin position="367"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="12">
        <location>
            <begin position="738"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="12">
        <location>
            <begin position="1146"/>
            <end position="1348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="20"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="33"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8158442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1576" mass="180588" checksum="A5EDF12768D6EEAE" modified="2022-10-12" version="1">MPPKNTQSGFMSVEQRSEIGDFSRHTPARSLAQPRNRSILATASRRTGTQSQHSPTRMTVETHEAVYWGTNIDERAIEKQFDRFLKEYRSGGMEYYMSQLNQLNETDQFILNIDGRHLLEFNNHLYQQLIHYPAEIIPIFDTVVQKVFYDDFLSLKARNEQEREEFRLYAQRLLIGIINLERNVQVRELNPKDINKLISVTGIVIRCSELYPDMKQATFKCTKCGHIVGVNIERGRVEEPISCQRCRDKNSYELIHNLCQFTDKQYVKLQEQPENVPEGYTPQTVNLVPYDYNVDDVKPGDRIIVVGVYRAAPIRQTKNRRVLKSIYNTFIDVISYQKETKIEQEKTKNITEEQKQKLMYLSQQPNIYDILVKSIAPSIWEMEDVKKGVLCQLFGGTNKEFSQAGKGRFRADINVLLVGDPSTKEXTRMTVETHEAVYWGTNIDERAIEKQFDRFLKEYRSGGMEYYMSQLNQLNETDQFILNIDGRHLLEFNNHLYQQLIHYPAEIIPIFDTVVQKVFYDDFLSLKARNEQEREEFRLYAQRLLIGIINLERNVQVRELNPKDINKLISVTGIVIRCSELYPDMKQATFKCTKCGHIVGVNIERGRVEEPISCQRCRDKNSYELIHNLCQFTDKQYVKLQEQPENVPEGYTPQTVNLVPYDYNVDDVKPGDRIIVVGVYRAAPIRQTKNRRVLKSIYNTFIDVISYQKETKIEQEKTKNITEEQKQKLMYLSQQPNIYDILVKSIAPSIWEMEDVKKGVLCQLFGGTNKEFSQAGKGRFRADINVLLVGDPSTSKSQILQCVHQLSSRGIYTSGKGSSAVGLTVYVSRDPETREIILESGALVLSDMGICCIDEFDKMDDNAKTILHEAMEQQTISVAKAGIVSQLNARTAVLAAANPLKSRYDVKQSVVQNINMPPTILSRFDLIYLVLDEFNEKRDEMLAYHILNMYSLKDKQDYLNQIEEEGNTDIIDRETLYSYICYAKQNIFPRLTEEAQNELIAAYVKMRSAGNSSNTITATPRQLESLIRLSEALAKMQFNQRVENYHVQEAVKLMETAMKKAALDPITGKIDMDLLATGRSNASRELVSKFIVEITNLIKANLSDYRGQGVRFFDXLLMLYHIKKKLKLNKKKLKTLLKNKNKNQFKSIAPSIWEMEDVKKGVLCQLFGGTNKEFSQAGKGRFRADINVLLVGDPSTSKSQILQCVHQLSSRGIYTSGKGSSAVGLTVYVSRDPETREIILESGALVLSDMGICCIDEFDKMDDNAKTILHEAMEQQTISVAKAGIVSQLNARTAVLAAANPLKSRYDVKQSVVQNINMPPTILSRFDLIYLVLDEFNEKRDEMLAYHILNMYSLKDKQDYLNQIEEEGNTDIIDRETLYSYICYAKQNIFPRLTEEAQNELIAAYVKMRSAGNSSNTITATPRQLESLIRLSEALAKMQFNQRVENYHVQEAVKLMETAMKKAALDPITGKIDMDLLATGRSNASRELVSKLIVEITNLIKANLSDYRGQGVRFFDFVEQINTIIAAQTNEQTIIDKRQYQKEILEALHLLEEQGFVQLPGDRNKPKIKAGIKALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U4S9</accession>
    <name>A0A8S1U4S9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0340030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8159860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U4S9"/>
    <dbReference type="OrthoDB" id="295179at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8159860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="296" mass="34730" checksum="9FF1AABA1B901866" modified="2022-10-12" version="1">MSSSNNQKQIKRRNKSYIHITNHQITEQCKQRRKSCSCLQCGQLSSLQFKFMNLQSQILHFSTNQHKKYQSIESTYIDSQDTIIANNQIHKRNTKTIQIKQHNSKNNNISLIQTNSNNFYQKQRSNSCYCDECGNITKFQSQAKLIYTQQIIKSRHTNFRIKRNLDQMFKGISPIERKQEQEIEEDENNPLQPNQITSFRRSFIINNTPNKQIQKVQQTSGRKIFLRDACKTTFFLKTNSNSREFTMKSESNSPNIANKKLNLPIVKSSYSKNRQVSNEVQLFKFTPKLNSINLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-02-22" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U8R9</accession>
    <name>A0A8S1U8R9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0360235</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8161535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1135" mass="134722" checksum="FF2DDB3B57D0E6D1" modified="2022-10-12" version="1">MQQLSAYTLLQEFEKQSELDISSLRNEFQKDSTYVIEVCANEQKLNNVAIFKNPFFQNHLLTENSFLQIIKQGKVDTQIATMGSPYAITLNEKNLKNQDDLYKFIVRNLDKTFTVQEIRHNFYTVSVSFCEFLKSFIISSSGQTIICEDKNDISQYNQIQGYERACKVAILFFNIHSKMNHNQQINFAKDLIHRSLVGCRNSSNFIEWYTIMEHYQSQRILPPNIVKNFLNHYNLEFAKSNEQQTNLLNFPSIYDSIYNQIIDKYHDYYKGKTIYFWKDDKYLGNCYIPSKHFEILRQIKXNYNINLIFHFIPFNLYISLDQINHLIVFSFLNQFLKLFIILLLLKIMQQLSAYTLLQEFEKQSELDISSLRNEFQKDSTYVIEVCANEQKLNNVAIFKNPFFQNHLLTENSFLQIIKQGKVDTQIATMGSPYAITLNEKNLKNQDDLYKFIVRNLDKTFTVQEIRHNFYTVSVSFCEFLKSFIISSSGQTIICEDKNDISQYNQIQGYERACKVAILFFNIHSKMNHNQQINFAKDLIHRSLVGCRNSSNFIEWYTIMEHYQSQRILPPNIVKNFLNHYNLEFAKSNEQQTNLLNFPSIYDSIYNQIIDKYHDYYKGKTIYFWKDDKYLGNCYIPSKHFEILRQIKMYINNSKSNFYMNQPPNFLSQFNLNQQEYEFYLKCLNLMFSIPFNKRITYKFLELSTLVFNCVHKRLTNIPQRLDYQLSLAQCLKPQTTIIVLIPVGINGMGYDEICQHIIKMCEGVKIVSKIDQVNDNQILYLFDQICSLKQLKSIHDQLKQLPYDIRTIALYPECSHYYLSEKQSRFPFSFKFIMFCLLSVLDQRDQVNRIIKQLKDFENQYLNHLPSDYQISCHYIPQKKEADDLYGEIVESDFKAAFSFTNDYLIESLSQYKDYVGNIPKGYLLDQAKKIINQIEDKVKINITKKKQLLYNKTNSKLEKQYVLYIQNPNWDDIDNFIFDCLEIILKNYPKDTYANLMYQQLNQQFFQKRNTQTYQRENQPYMLSKKSEQKERQVQVQVAVIVVDGIIILQPQDLGLNLLQDIPIYSNNIEVTNSERIAQFVKSKVEKLYDKQTKEETIFQLDLELNYKVWRVYLVKLKPIIINLVEGLVERLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UA67</accession>
    <name>A0A8S1UA67_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0350067</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UA67"/>
    <dbReference type="OrthoDB" id="293020at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398:SF0">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="48"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="337"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="663"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="669"/>
            <end position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8160599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="684" mass="80289" checksum="FC4D1745FAF8BBC8" modified="2022-10-12" version="1">MKILNEDIKLQKTSLMNAKTFSHYFKQIDLFTKPVQLLIKKEEGHKTIMGATLSMIVIVVMSIFFVNSIIVYADRSKPVTLTTEVYHAEPNLYELRPNNFTLQFGFQDSSFATYIDESIYFVEPLLVSKSVEIINGTQQVRYNYVELPLVQCSKDIIKQDSLAEYFSHFDLSTNYCIDWNTIDKINMQGTFDAVNYQYILLKFKMCTEDNKKKNVPKCKSKEEIMKKLTHNYLSLQLSSYYVDLQTTQNPYIPKGEDLFTTISSQIFKEITIYLQPVTTYTDSGAFFQDFHYDTTLKYLHHTEMIDFNEEDLLANVAIRLHTIEQVNYRQYSRIQTLLAELGGLWNVLFTIAMLIQIPYSTISYKLQIINSLFNFDGQEETINDDQDNYLLDQQDAPIINSKQSSKIYVENIQKNTLNDIQQTNRKLISPIKQNTPLKKSKFQRQDSFRSSIKSSYKVQQKLQELNQQCMKIQNAATLDEDIKVKTIDKLNQQVKGFFQTVSKKLNIPYYKYMLSSLKLYQDDQQIKQMNFSINRMEKSFDILYIIKKLHEIDKLKMILLTKEQIKLFDYLPKPTIASNPEKEIQGNQYFSILKPLKSNYQMALEAQQAYKEILFKMHDPINKHLIDCMDENILEFLQFQLYRNDGEQQDDDILNDGTIQETKQKRVNSEQMLDNFDNVSQQNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UBA2</accession>
    <name>A0A8S1UBA2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0370215</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8162130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UBA2"/>
    <dbReference type="OrthoDB" id="8170117at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.30:FF:000001">
        <property type="entry name" value="MOB kinase activator 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005301">
        <property type="entry name" value="MOB_kinase_act_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22599">
        <property type="entry name" value="MPS ONE BINDER KINASE ACTIVATOR-LIKE MOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03637">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01388">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8162130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="220" mass="25567" checksum="419EEEDF1A4C5C7C" modified="2022-10-12" version="1">MQPNDPKTYKPLKQIDKNQRGYGLKQIAQMTLGSGNMLLAVELPNGEDLNEWLAVNTIEFYNEISILYGTLVEFCTPELCPIMSAGPKYEYLWADGQNVKTPLKVSASEYIDYLMTWVENQLNNDSLFPCQIGIPFPKTFLSVVKVIFKRLFRVYAHIYHSHFQHIMALELEYHLNTCFKHFIYFIDEFKLVEDKELAPLAELIQQFKARKENPTMNQGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UCB0</accession>
    <name>A0A8S1UCB0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0380057</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UCB0"/>
    <dbReference type="OrthoDB" id="289129at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8162480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="221" mass="25953" checksum="874D7D19AC086751" modified="2022-10-12" version="1">MKNQQGYLSEQDINQNHKKCSSCYKMVNEKRNSLLVSLAQPNRNKNTSFVPEFQEMPILQRRKSYQEGISGQKMNRLNCIYPQSICMQHAIQKSKIDNKATLRKAVKRASMMMSTVKYWKDHSQKKIEIPTIIKSIHTKDSCLYDKNALTTRSMKKNELPPVLLSQFRIRNNEKLIKYSRDRTADKTKSVSHHHFSNLQTQTIKNFSLRYSIQNANIRIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UJD7</accession>
    <name>A0A8S1UJD7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0420081</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UJD7"/>
    <dbReference type="OrthoDB" id="286647at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00198">
        <property type="entry name" value="vWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8165178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="428" mass="49421" checksum="8E03736320402A50" modified="2022-10-12" version="1">MNQDKQFYLYTIVDQKPDNNFSVVGVIDGSGSMSECWEDLCKAWNNFIQDIQSSYCIQFDDTAILQQNPKLKEQKGEGTNISLGFHELIKLIKSEKLKKNVIVIFITDGVGEDVLEDYIDNLAENFSKFHEEEYQFKFFTLAIGETFSHTIVAELKNRIHNSTLISSQMEYISNPKVDFENKLQQIKEEIFYKLVRVEPPVALVPYGKKQRRLAPNQTVFSETPIIKVNGEDINLKQYQITYHDLYQFVNQIFLKLMEDSTRGLKTVKDEATKQLQIINALMKRVSQEDGSDYKQDIDETITKIKAFASGSQQLKLLSAKDAAQSITKLQAAILAKNFKRQDNYEQKPVTNEKLEKAKQKLNSMKKSVKIKTIYPQIEQFFKENSNASPQLLVKLLQEKRAGQNLQRYALEYQGECTLLFELLYICNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UK44</accession>
    <name>A0A8S1UK44_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0410081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UK44"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="70"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8164533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="121" mass="14446" checksum="463EBA1D55CAF013" modified="2022-10-12" version="1">MNSNQFHEDGDPQQQFLEAFQELLDIQNQRIQNGNDLINQYCPQLLYPIIVPTQIPNTFIINMGPYYPHIQQPQLHPQINQQQRTPQQPQQNINQNRPNIKEEELDDNKQFKGNWLQQKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UKH0</accession>
    <name>A0A8S1UKH0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0410138</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UKH0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="520"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="9"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8164647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="695" mass="81745" checksum="3581EF5FE35FD540" modified="2022-10-12" version="1">MMANFENHQNTQVPQSQNQMQNQQEVATFKNTKATIDLIALEKQFTTPSKLQSLSVLKFDIGYINQNIIPNQKKNPEITNFYKNLVSELQQQLSTITQNIKDEQIEEIQYMKMVNTTLQEEQLKFTIATSVMKNRIGLRCRTLQQEIQGLQNNKFDKICQEFGCGPSLTQKLLFQEFQDKPFKQKAKELFIRHKEYLNLIQYLSTHLKEEKRELIEFLIGKLEKSKAFQNDLKEGKNIDYSKFYISEFKEITEEQFLGISKKEYLEKIQNMQDQVNQAYKEEQLYYKVASLAKPIVQIIPGTNQMQRISPADICKQYFARYEELQKYLTDQKDVSWTPLPIFKLDTENREISTVNKDVQNGILRIQFKNFINGDKFFIQFELLIGNQVYQAKTEQADGQGRLNYNQDINLKEFKNGTKEIHLSVIDIKVFNKGWLSNDLKGQGKIELNDLLDMSTIEGDLQLNEKTVLQYSIMLRESINRKKTVTTAQILKTYPKFDKISGLDEYLEQKLKEIPNEPIQYSKLEIKKLQQQQQNQIEEEECQGPQLQPDQLIILNHQEIDEEFLNVKKLAQQMPQLEEDLLNPDNPLKGSVYYFLVQYSSFLLQKSVELKQDPSQKLHSRFVQKLGEDCLRQQNKLKKKLMDDMEGYFDDLQKIIKKDQLIIKLYKQALAPNWQKFVEGRLEIALQEEKAIQEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ULI1</accession>
    <name>A0A8S1ULI1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">1-phosphatidylinositol 4-kinase</fullName>
            <ecNumber evidence="2">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0430133</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19877"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.67"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8165800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ULI1"/>
    <dbReference type="OrthoDB" id="10264149at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00893">
        <property type="entry name" value="PI4Kc_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000016">
        <property type="entry name" value="PIK1p Phosphatidylinositol 4-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF22">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="5">
        <location>
            <begin position="414"/>
            <end position="674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8165800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="690" mass="80520" checksum="6D1139DF35472108" modified="2022-10-12" version="1">MSKFKDKPTSSQIGCGCLGSSKQKTMMKFSHIEIDEEIVSASIIMEMIYRGQNAELALSRCRLNPNLVLLQFRDDLEYFIPQLINHIIYKQLEYVLNFILKAAEVDFFFAHTFFFAWRSIVNDNNPEIENYLRLFLSTLDRVYKKTLLIAACLENHQFNDGIEQQVSKGVPIVQQVNKLPDRIQAYGTLFYSNHVFNDTLQLANYESIFRQAKPSGFCSTIDFWDDIIYISQNLHLADPKLISLKADIQKINRGLPSAVYIPFQGVRNYVVLNIVVDECRVFSTKCRSPFYLCFEIYRPEEEAELIPNPLRNSRLSIPKTVGKMDNKNNSENNETQDCTRNRPTYLCKIVEAEEDAEEGKEDLTQFSNILRDNDVTHSYYEDLNNLVSIQNVKGLQQQLKKMTKKELTDSIYGCDNQDKIKNNSPFRNFKTWKLVHLIVKSGDDLRQEQFAMQLISTFDQIFKIEQLKLQLTTYEVISLGPGYGLIEVVRDALSIDSIKKKLNDILQIKCLSHYFKLNSSQAMISNFLNSLVAYSLVCYFLQIKDRHNGNILLHRDGNIVHIDFGFFLSSAPKGNLEKEVPFKLTEEYVEILGGYDSNLFKRFRKMFFEGFKAIRRHKDKIILLVKMMENSSLNCFKENTLLNLERRFLEDDLSDTQLYVQCQKLIDLSRGNWRANWYDKYQYYFQGIMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UUA1</accession>
    <name>A0A8S1UUA1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">RCK N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPENT_87.1.T0430251</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8166036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UUA1"/>
    <dbReference type="OrthoDB" id="10035564at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005267">
        <property type="term" value="F:potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000146">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000797">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003929">
        <property type="entry name" value="K_chnl_BK_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047871">
        <property type="entry name" value="K_chnl_Slo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048735">
        <property type="entry name" value="Slowpoke-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027:SF10">
        <property type="entry name" value="SLOWPOKE 2, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03493">
        <property type="entry name" value="BK_channel_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22614">
        <property type="entry name" value="Slo-like_RCK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21014">
        <property type="entry name" value="Slowpoke_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="99"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="204"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="228"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="14">
        <location>
            <begin position="626"/>
            <end position="767"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8166036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="990" mass="114130" checksum="36B060F0C1E484E0" modified="2022-10-12" version="1">MIYEGKQSIFSHQDQYALIRLVNSEDDGTTKKHEEGKTSFTLKLAKQLSTSGIGDYLDSINVFLSFLLPVIHLIDCYTWEQGGTQDVGEAQAYITIPELICYIYFLLDFAINFYLSENKLFFTFQTTSLVEYVSIFPSLLARLNIITGYKYIYLLRVLRFLLCYKLDKVLQRQSMEAYKPIVTVMAIIIINSSILYVVEQDYSIVEYIYFMVVTISTVGFGDVYPTTIYGRFSIIIAILIMFLVLPTQVEMLTRVYSLRSQFARNKYITKTEQEHILLLGSSQVEGFKTFLNELYHSDHGLNDINTVILQNQAPTEEMTIQLKQPALSQRVIYLEGHPLQNKDLERCSSKDCNCVIVLANKTSHTPKRDDYRNIIHAFAVKQYAKKQKSRKGARVCLQVLQPQSKDLYFNSLGGHETDQVICVDELKLYLLGKTCLCPGINTLISFLIQSSKPSYDITKYDKNKSEWIDDYLCGMQNEIYRVPLESEAFVGLTFSQISQAIYKELNIILFALEVELESGTNVFVNPADYLFEDYLHYGYVIATEMPSIEQIQQTKFPDYIQKNYCFPNVQRQTNNKNALQQETEYLKEILSEGIKDDSQKHTSYYQVKPQTITTGLPKVGDQEKFENHFIVCGVVTDMKYLMMPLRARSLKNIQPIIILNQDLIPTEIQLQINKFPKVYFLQGSPLNTEDLKKACIQKASALVILQKQVDQEEGISNIVDADTIFIYKTVKLLNQNINIITELASISTISFLQISRNNYVQKYDWSVSEPFASGEIYISTMLDTLICQAYYNPFITSIFDQMILGSASMNKKNKKLYQTCKLQQSNLFLINVPPKYQEKTFGELFEILLTEQKMIPIGLYRGEKVKNNNKPYVFLKPPMDVVLQNKDRVYVLSAKQPKEIETIIDNEPNQGQDNTAQYFNIKSKLQGEEGRVDIEFSRELMRFNDRMKKLNKQLNNINQKLFNQGLIHEDFISNVRSILKKELANLNPGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UUV7</accession>
    <name>A0A8S1UUV7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0480161</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000048">
        <property type="protein sequence ID" value="CAD8168047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UUV7"/>
    <dbReference type="OrthoDB" id="311354at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="84"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="147"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8168047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="214" mass="25880" checksum="BDF94CDE3295D1EE" modified="2022-10-12" version="1">MEQPEKIQEDPKMLLLTMELNRMAYERQIIKSKEEFQKNNNSNKDNQQIKEYEKQMKQLKEALNQKDKKLEQLVQELNQEKQKNQQLQKQIDSIQKQIQQTKEPKIHSQADQKFLQQQLLQQKLQQQTQKAQPQILMQTVQQKGNPNSKQNLNQQQQQQPPIIQQQYSQQLYQHQQQKSSQSKHTSNQQKQMYQPQQSQYPQQPIQIQYMQTRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UXY9</accession>
    <name>A0A8S1UXY9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0500174</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8169127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="299008at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="83"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="171"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="219"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="273"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="297"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1082"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1281"/>
            <end position="1302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1384"/>
            <end position="1406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1596"/>
            <end position="1617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1032"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1034"/>
            <end position="1048"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8169127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1660" mass="197023" checksum="C009459F24A2A95E" modified="2022-10-12" version="1">MRQIIKKEDSNFLNQIVCNMLQINDQFYCYEPFKIFIFIVSMCQPVFYLYYYDYDQFQEKIPNTTIQQMIFYFFRPEILLFKYFPNIVILIIPYIILVIIFIAKLGIIFNLANENQRYSNKQKNPSILKNMFITFTSYYQQLLCIILHYPIQTLLICSISNTLKQFQNQNSAYFALLLFGIIIFFIIEIETLLNLFICFPSSNFHIKGFEKSLVTSLDIIIYAIELLQIILFGTIIKQEFSQFAQIALTLIIALLKIINQLMYQGYIYKTQRIILYFINSMIISYSCYAFINLKNNSIIIWPLLASIILYIDYQYQINSIYSIFINTKQPITMYVYQLIKFSNNCYQNEIQPFQNSLIWSQHKAKCSNVTCSCKDQTFNILDVKQANIITEQIFKQFIYTKIKHITKQIYENRNIHNLDTAFYEIAQATLFMRQGFVLIAIKNYNRFLNNSSNDYRVRVISSEKSLTNRPQKKIKQSQVDSQIQDIINNQHKKVLKYDVINRNFQVSTLKLIKIQYLLKQAQVYLNENFTNAICTAQQLQMSEYLNFYLKSEFQMDDLVQMIIKIIKLKIEFYTYLLKCDAMNADKIFIYKMKYFTQVLDLEEKLLQKYEAYQSIKMKQMIIFFYSRIYNSYQKAQKFKQINNNQQQKYIFSNQVIDFYSDKIMYVLFQLQDDLQNLRIKSHSSNFLKIIGRQPNEHLIQFNDILPEFIREEHPAMVQRFVQTGKARYYRNLSLTFVKQQNGLSKQMEQTFDTVTLLNDNRVVFASIIQDVLEQKAYLLVNVNGLLSGMTFMCLRKLGFSEEDILNIDEFYSFYEVEINQIIPVFNNIPWSELQEQKLDNVKFLFVDILQFQRENYSKSSIENHSQLGRVWQDSRYTKEYAANLFIMKREIFGFYYFIIELESAHLIESSHQNNAVFSCTQFKQTQHNAESAIFDIDSIILSIDEGAAKHVNTLNIQERENYSIALKELSKQQDFDKIYQVMLSPNNSVSNLIDKSQNMINTVQRHNSSFQQEYYCQQPAFYNAEASSKEKHPQLQQVQKQDDLQQQQSESSLGSIKRPIYMKKFEIIDSFYSNFNFSQQQIMVFFIIFTLIVFGIFSVIILSLLSNDLQTKIQEIEMLSFQADIVAPYDRYLAIRAQIYYYQGLYTAKKISQQQTSDLVEPLYSIIGVCYNELKQNTYISLLESDLKEFFKDVYTNTFFMGMTDKIIYSKNITFREFTHQLLNYQYDFKIIFDKRIPTKGCPCQVFQFSNYFNLQDNLELMSQQILEFSRDKGTQIIDKWITIWIVFIVVCFVLSLLIYYLKTGILFQYDTIMEIITHITEQSILKETEKHKILLNNLLSQTDYINSYQLELQTEIQQNIQNKGENDVLRKNKRKILIRASKFRSIIFSFVIFVIFLIYSCLITFQTQSFLNKYQSTADFYKLIADLSFRSGNMFLYREMFMLWGNLTYLNKTDGLRLYNLIDIAQSKIMEYVDQAPRINLETLLVPENFYEFFLTVQNTDVCNFLDENYKRVLTPYCLKSFDGSLMKGMLPALTYIHQTIITQQAINNFTYRAEDILYEVEGGQVASRVFSFMNENLQKGIINKTESFNYQNQLLSIFFLIALGIICFYVVNFHYTNLKQHLQLIKFGVLMIPQSDILKNDFFERYLKQIELKLGYKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V047</accession>
    <name>A0A8S1V047_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0520242</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000052">
        <property type="protein sequence ID" value="CAD8170275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V047"/>
    <dbReference type="OrthoDB" id="10371026at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8170275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="265" mass="31036" checksum="F96E5B1B7E5421A5" modified="2022-10-12" version="1">MQQNIVFDISSENSSSSDEDEFIKIDQQNSSIISVRAPNLFDSDTPQEFQQIQVDTHSTRKQHTVTFDTLNTNEGERRQLNKWNLQEKENKNYNSFTIKTELTEKGRMNSKKNINESVEYLQNQKFSQKQQHKFNQYKKPYQPKRVANVNYSSIDNFQNQSMAQELLELDLPALISQITNKRRVNNNNNSFHNHHNNSFYNSNPKPQITQKQGCIIRQSFNKQGSQVEIPIVQQTRERSITMILRQPGKIVNQKQLKSNSIALRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V1W5</accession>
    <name>A0A8S1V1W5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CSC1/OSCA1-like cytosolic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0540240</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8171330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V1W5"/>
    <dbReference type="OrthoDB" id="292950at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF83">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="414"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="448"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="541"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="598"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="2">
        <location>
            <begin position="219"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8171330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="880" mass="102752" checksum="DF05BA3C00B8B894" modified="2022-10-12" version="1">MNQIKIDAENSLTFDPFRIPASIALAKIHQKASICGENEQKSDHCPCCGQHIQQREISLCQNRLDLAFLGQGMPLFFEMTSQLILIVLILFTVLGIPSLIANIQNHDCIGEENMFYKLSLNGLCKQNCPQNTTDFLSITKLVHSNDCQTICAKIVDICLETSVLKMSYANKQFDETSKLVQSILVLCSILMFKFAMIRVRISQRSTVRICDQEFISPSDFTAMLTNLPINEYNEAELKDALLDFCYQFDEKGQYEIVKIIIAYDITSFVECSREKMSLEKKIEKIENYYKQYGRYPRSLKTNQFSVLKERIMVLDQKLMTIENEVENQQYAHQTQVAFVTFQTKEQLQNVLDQTKLSYWEELWIRFKYYLINRQDQRGFYFKNRIIIFCRAPEPNDVFWENCGFNFQYQFQKRILNFFITIFILGASFTILLGLNILQSQNLSSFDDVIMIIVTLVISLIITIINQIIYYFIKLLGQSEKHFTKTHHDVSVATKLAIVQFFNSGIFTKVINILVYNFENDKADNYAATYSYSRQGGVISDAFFLLIVNSFFVPIFAYLDPLYLYKQYQQYFFKVDNNFNQIEANKIFEGPSVLLYEQYSYICLSLWISLLFAPLLPISLLFCSSGLLLYYWIQKYLLLRRNSKPPFQSFHLNREMMNLFELSPIILAVGQMWTDYIFNSSSAILTVNFISLGFSCLELITPATRISKLFDKKGNIFTEKDRYQDVYLKLPTDYDRTNPLTQQNAILEFIKAKTLIDTNYIQPQVMDTKTALYKYAQMGGVQFMRNTISLKLKISMMRKIKLIRKARQIAAVIQQQQDEEISQDQQEPIYSQTPEYMIVNKNINLRKNSLNISTDNDYNNKFQDAQNYNFSKFLSPNKFEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V1W6</accession>
    <name>A0A8S1V1W6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0540254</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8171358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V1W6"/>
    <dbReference type="OrthoDB" id="288218at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8171358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="156" mass="18631" checksum="C5C21EEB6994EADE" modified="2022-10-12" version="1">MFKIFRLIETLGRFFKKPPAGTGRRIAMSPFFKNILTEDEEENFKRQEIFYDPHTYYLRQQKIQSYQNELMQKNHQRRDDSGLSELVITAKMPERNQLSHTGDYQIHNQDSFLHSIQLGRKGAENHFFSLDDVESIYSSFEQDINFDIEDSIVYQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V3J2</accession>
    <name>A0A8S1V3J2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0530031</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V3J2"/>
    <dbReference type="OrthoDB" id="422728at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001293">
        <property type="entry name" value="Znf_TRAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131">
        <property type="entry name" value="TNF RECEPTOR ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131:SF94">
        <property type="entry name" value="TNF RECEPTOR-ASSOCIATED FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50145">
        <property type="entry name" value="ZF_TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="77"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="TRAF-type" evidence="7">
        <location>
            <begin position="172"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TRAF-type" evidence="4">
        <location>
            <begin position="172"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="285"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8170382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="439" mass="52621" checksum="44233E23806A2D19" modified="2022-10-12" version="1">MYYPYAIEESDENISQIVSNQDLEFDEDEDFIQDSYQSPERTISSNSDNDFKDEQFVDQSRIINLSEVKVFLDEVICPICCHIIIDPKICSECDQTFCGKCILRWFQKSPNHQCPCCRKDLKSKNGYSYNQSIMDDKVPKVMLKLLSKLMLTCRYRQDGCEEIISYDFREKHENQYCQYQEQCCENVGCYETMLRKNFEDHQLECRYGIVQCKYCQEDKLRMDIELHLQQCDCRPILCEWCQEKYQYIEIDEHRDLCEFKNILCEYCNKKYKKYDMKTHTPIFCLTQLNKQKDQIILELKKENQQLKQNKPMEQSDLNQSTNEILSEKYKQDFEEEVEEVIEENIQIEDSDQEQPQQYDNNDQDISIQDQKSQSLSDDENQDKLSNTNEKLDIVEIPSIQEIKMRGMFTSFLEKLTDIKFKDLWDYSEDEFNQVIDLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V6Z5</accession>
    <name>A0A8S1V6Z5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0570208</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V6Z5"/>
    <dbReference type="OrthoDB" id="306643at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8172664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="71" mass="8395" checksum="A95F0096158B8F74" modified="2022-10-12" version="1">MQQTVSLFAGMTKVFKSANNNKQYQETCKCQKENFKVESQNKEHLQEFSKKHSTNENQKENIMIQSQPNRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V985</accession>
    <name>A0A8S1V985_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0580255</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000058">
        <property type="protein sequence ID" value="CAD8173181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V985"/>
    <dbReference type="OrthoDB" id="293086at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035720118" evidence="2">
        <location>
            <begin position="16"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="51"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="280"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8173181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="363" mass="40755" checksum="94E4D1BC1D213796" modified="2022-10-12" version="1">MKYILVLALITFTCATSLTEMSERLSQYGDHPFGKSMVNLVTVNMKTGGSLNELKQLLQQIKDELIALTQLQDSENGTFTRRSQVDLAKLQATLEQVQGDLDNQRQEQSSLSNELATLQTRVKEDQAALDRNSRGSGDAQSRLDAENADFATKYSDYNDAILACKEAQRLLMNLRGEGASLIQLTQDTKSNLIQTKENFQKIKEILEAHTKKSSLTLFQPIIEGLAEMTTKVNPETLNNVLSLVARLITALQEGQDQLESNHKTQVENLTRLGDDLRNEKQTLQVSLATANNRLKEIQSRLNELDGLINISNALVEVTQLNIQDATKINELEDAEYSNQKVSRQTEIDIVDRLIEYINQKLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VE07</accession>
    <name>A0A8S1VE07_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0610234</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VE07"/>
    <dbReference type="OrthoDB" id="298511at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035748476" evidence="3">
        <location>
            <begin position="16"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="178"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="66"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="357"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8174695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="409" mass="48239" checksum="FCC4033C90FD695F" modified="2022-10-12" version="1">MKQFIYVLLFIGVMGSDLESTLKQMKQTEFGRSFLEKIQINDPVEKIIRDIANFYNNVDRRLEEGVQQFKQSQEKCNQEIKELKTNDEELNRKLRDIQITKTRKQPRYDLIIHRFHQKNKMRQELDHQETRYIELREAEQSFFNYNQDQLDKAMVVIVEIRNKFIAYLSQRQNRQRKAAVRDNQYWDDDETPFNDDDDYDEEEEERQHNEKYAFIQIIVAIQRVKTGLEMNGVTQLVQGIEDLCQQMHDIKEGNIMYDDSEETLNKLASQVIDITSSVLDWIAQVKRREEKADDSKGDVIIQTLKLINKQKQQADVEIGQLGNESAALSQQLSSLEQSEDLTRFSINNNKDLLQTKIEMCNDQESAYKIEKRQLEEQLNTVGLLLEAMEQKGGQFKRVMKTLEGVADYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VET1</accession>
    <name>A0A8S1VET1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0620055</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8174905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VET1"/>
    <dbReference type="OrthoDB" id="296474at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05380">
        <property type="entry name" value="CAP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035764341" description="SCP domain-containing protein" evidence="2">
        <location>
            <begin position="16"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="3">
        <location>
            <begin position="29"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8174905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="238" mass="26760" checksum="D596C2D9131976CF" modified="2022-10-12" version="1">MRIILSFLTLFFVNAQNQYCNVHNKGVNSGLANQLAHSLNQIRNQVATGQISFEGSQSASNMNVIHWSNGYAKMAQTCVEKCPLKASTCGQFQNYGVLFFKRHLNQQMRTNQVMEKWFKEPENAKQLLTARSSAFGCGKAEDNASEYIVCYFDEKYTGAKQAFLAGPVGASCKLGRSKMYSGLCATASSQTLIHQSSHVKNKQQKKQKKQKKQKTHKNKKNTEMDVALISMTDFKEFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VFA2</accession>
    <name>A0A8S1VFA2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein arginine methyltransferase NDUFAF7</fullName>
            <ecNumber evidence="7">2.1.1.320</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0610181</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Arginine methyltransferase involved in the assembly or stability of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)'-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48108"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:88221"/>
            <dbReference type="EC" id="2.1.1.320"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NDUFAF7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.320" evidence="7"/>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VFA2"/>
    <dbReference type="OrthoDB" id="17415at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035243">
        <property type="term" value="F:protein-arginine omega-N symmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12710:FF:000012">
        <property type="entry name" value="Protein arginine methyltransferase NDUFAF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003788">
        <property type="entry name" value="NDUFAF7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12049:SF5">
        <property type="entry name" value="PROTEIN ARGININE METHYLTRANSFERASE NDUFAF7 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12049">
        <property type="entry name" value="PROTEIN ARGININE METHYLTRANSFERASE NDUFAF7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02636">
        <property type="entry name" value="Methyltransf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8174589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="390" mass="46061" checksum="98268F82D0DB6A5B" modified="2022-10-12" version="1">MKRMLVRDFIYDRLYHPVEGYFVKNIQLGALKNPIEFKQLLGYEDYTKKLAENYPENQWLTPSEVFRPYYGITLGNYINQQFRFTRKEKLRIVEIGAGYGAACEGVLYYMRNHQPQTFSNLEYHLVDISPEACVQAEKRLSQDFKQQIKKGNLRIFNQDFLNYKQHTQNNEMWFFVFLEVFDNLAHDKVIDGKQVYVENMKEYTETITDPLINEVYAMYQQFKQQNKNQDENIEDRFLFNTLRKLISKYYGNQKSNSIFLPTGALQVLKHIKSNFHNPSLVIADFDLLKNNFTQESINAPIVSKKLTKPHERLDYESYLVERGAADIFFPTDFNFVQYMVKQILGLDSQIFKAYQFADQFSQSSWTTTKSGYNPLKEDFGNTSFLVTDHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VG45</accession>
    <name>A0A8S1VG45_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trichocyst matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0630148</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VG45"/>
    <dbReference type="OrthoDB" id="298080at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035784751" description="Trichocyst matrix protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="257"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8175621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="359" mass="41162" checksum="0DA445E80D347323" modified="2022-10-12" version="1">MKILLLVLIGLALCKLGTDPKIILTEIDDHHLGKTFLNAIQISLATGSPVHEIQSYINNIRFMLEQEQKDADLFIQNTQASCNRLLHDFSTNLAYHQSQLKAHQKIVDENTNNLQRSLNKIAEVSVEIEENTKKTNAGQSERDLQYAEFQSKIKDHTEAISAIDEAYALIEHLSGGSSFIQVKGRFNKVLQRLQNQSTGLLFQPILTMMTQLSSKSDSDTAKKVLQLLANLRVQIVESKGNDESIEKQQSLNWQQFLADLTNEKNTLSDQRQNLEQAILNYQSIIEESEGKVEYHAAEVERNQNNLEGQDQWCRQQQDIYQMETQSRVQTQDLISRISDHIQDKIVTLKEYLRERLQLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VHG8</accession>
    <name>A0A8S1VHG8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Syndetin C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0650219</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000065">
        <property type="protein sequence ID" value="CAD8176710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VHG8"/>
    <dbReference type="OrthoDB" id="298507at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990745">
        <property type="term" value="C:EARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019514">
        <property type="entry name" value="Syndetin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040047">
        <property type="entry name" value="VPS50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13258">
        <property type="entry name" value="SYNDETIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13258:SF0">
        <property type="entry name" value="SYNDETIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10474">
        <property type="entry name" value="Syndetin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Syndetin C-terminal" evidence="1">
        <location>
            <begin position="738"/>
            <end position="963"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8176710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="978" mass="116267" checksum="5A0A99A7995C4CEF" modified="2022-10-12" version="1">MNVKNYILRKLVGDSSNTLTIEQVQRILESQKDDDRIFRAIINSSLSNQKLQIANSEFRINSLTDFGNLLLNQLQSSLQSIKSKNQLLSIDQFPIDINQFNIEISQTDLIKQQLQHIYRLFKATIRQNGDGLKETLNYLANLNQAEQIIESQYAQQCILKAPEYIQGIDEINLWCQKSTTLYEAVISTRQLLQQSKSTSLKKQLQIVDKYKTIQRIKYTLEKLMILKQTFGQLAQAMNKYDDNAYEIIPIMKDTQNNINKYKLNELKLSCISDNFFENKIESLKERIKQGILANFMKFDEKKYYSLLKSYYFIEKHYQMQSYLTQIWALVKKSISVTFKNAIKLFQQSYIPHKIYQNCQDEDLLQFYKLLYSELVEQMLNFHYCNRFHQENDFKIEEEIGNTQFEEYTEQIRQSYLEMRQLIWAQIQKKIVKIITAHQINIKSLVIEQLIYFLGYNCLLIKRAEDFSHSDSQNLTQCIIDVYMRFVQQFHKANLKSISTYLQNELYQRLPLTKDSKLIQQLEQLTNLNQVNKKVFEYLIEKREENYQQETKKTVDFQDLINNDKEMQLFYLFEYQNPFVLQQDEFDLIIKAIWIKKHTSISTGVLNQIESVLSSSSIRILELLPQYALLSFYHPSCQQQIIACFLQLLELYMFVSYNQLVGMTQQKQLLENFSQIIKFDESKEIEFYVQTYDSFSDSLVLQERFCYFKRFVQKFCKEGIDFKEIDLFSSKEKRQFVDIAERAIASESLAYLLSQTKSVFSLIGITSQNFDIFKEVYNQYKEFIYRNFVQTYIKIDGIVQYVPQQRWDLKDLHNENNNIYLDRLIQFILDQRERLKSIGGGVIPPNAQKDIFSFLAEHCLNVLLETYGKIKKLNSIGRDQMIFEYTTCCQSIESSQKNNKEVVLNPKITLMQLEYLKVFNSQQYDEIDQYIQKSKTLIPLRLLYNLMNSSSSYNKLSKQQRKDISIKFFKDYYDTFKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VI15</accession>
    <name>A0A8S1VI15_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0640116</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8175995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VI15"/>
    <dbReference type="OrthoDB" id="3045089at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047126">
        <property type="entry name" value="RNF141-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109">
        <property type="entry name" value="RING FINGER PROTEIN 141-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109:SF5">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="67"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="124"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="347"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8175995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="414" mass="48442" checksum="6B4F7EDF17C2271B" modified="2022-10-12" version="1">MQKEVVEFTFKHFVYKYTFINIIVLLFTLYQFQDAPLALPLGCMILNELTIWIKYSSGQITDNVDHLLHILFYIYITLIYYLELNYYIFSNIFIVLSFIFKIACDKKSDEQHEYTTIIRLSKIFYRFSQLIALFCITLKMNQYVDWTWSQTFWWYWMFLSGLIGSTITFLLILISKLIKINNSTIINNFKNEIKMLLWLLYIACLSSLIAGIWIVNTLNLLGNTINIQIGDIFMYITISLNILIFCTISYVLFNSIVEFVISINEIDSRDSSPNPTIKNTDQQIKKELTITSIFMQKLSNAYFKQMKDLEVVTLKDANQNEILTERNVNNNIIKKNKASPHNSNNKCIICCEKSSNAILMNCGHGGICYQCAVQLAQKQKECFLCRQIIKFIYEIDEKDVSIMKRVISKTRISN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VSK2</accession>
    <name>A0A8S1VSK2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0740216</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000074">
        <property type="protein sequence ID" value="CAD8180624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VSK2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="22"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8180624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="74" mass="8764" checksum="6F780759A2323369" modified="2022-10-12" version="1">MIREVELTYIYTLTYMEQSKSIQYIILMLCQLIQLFIFQMTNLINQRGELLQQKAKGLSFWIKLQSNSTGELVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VT71</accession>
    <name>A0A8S1VT71_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0710179</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000071">
        <property type="protein sequence ID" value="CAD8179295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VT71"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8179295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="278" mass="32742" checksum="9BD9DF4E9FA3FACE" modified="2022-10-12" version="1">MLYDQLNEASVRHVEFLFTLVQGNESECQIEENLIIYSLLQLFNLHFSQTNPTIVTFYKELTPEQFTKITFKNIKFNFIKGSNNGIFIPLFFKLQLLLKLENIIFTMPYNEYRNISLMIRTNIPYSLLIENFTIIHFYVSSSDIFSFMFIKNSKKNNIQINNLKILDSNFFNSTLFILQAKMTILLYDIFFNQTSIQNKIFSQSNFFHSYGLLNYTTCNLWIKQIMLLNVQILQNSSILLLCCFQQSQISNESALESCAKAFIENYQMSNTLKNDKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VTF4</accession>
    <name>A0A8S1VTF4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Calcium-dependent protein kinase 1</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="ORF">PPENT_87.1.T0720080</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cell membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Parasitophorous vacuole membrane</location>
            <topology evidence="24">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="30">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDO010000072">
        <property type="protein sequence ID" value="CAD8179513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VTF4"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020002">
        <property type="term" value="C:host cell plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020005">
        <property type="term" value="C:symbiont-containing vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000398">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000585">
        <property type="entry name" value="Calcium-dependent protein kinase-a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000299">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001182">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 26 27" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17" id="KW-0969">Cilium</keyword>
    <keyword evidence="15" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1032">Host cell membrane</keyword>
    <keyword evidence="16" id="KW-1043">Host membrane</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0519">Myristate</keyword>
    <keyword evidence="12 26 27" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0564">Palmitate</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 27" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="28">
        <location>
            <begin position="43"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="345"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="382"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="418"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="454"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00060437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00068067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="EMBL" id="CAD8179513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="497" mass="57110" checksum="C7F46B5352BCD1FA" modified="2022-10-12" version="1">MGCGSAKPSDVNNPQQINDKSQLRVTSLNLVTEKMGQISQDYHLLKPSLGKGAYGEVRKGIHKLTNQTRAVKIISKDKAKKADMERLKEEVDILKRLDHPNIIKIYEFYQDNKNMYIVTELCTGGELFDKIQESSSFSERKAAETMKQILSAVNYLHKSKIVHRDIKPENILYESSKPNALLKIVDFGTSRFYDPDVKMDQKLGTPYYIAPEVLERKYDEKCDIWSCGVILYILLSGTAPFNGDDDNLIMEAVKRGFYSFDTEEWRLISIEAKRLISKMLERDPKKRISAEQALQDDWITTYVKKPEIDLPQLTRVLNNMKNFNVEKKFQEAALTFMVNYLATSQEKQELLTQFQALDLNGDGRLSREELIIGYSKVMSYSDAEIEVDKLMKQIDQDGNGSIDYSEFVLATFNKVKLIEDKRLEQAFKLFDKDGSGTISIDEIKQIFGQNSQVSEKVWKDLIQEVDQNGDGQIEFKEFKEIIVKAIQNTNETDDIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VTS2</accession>
    <name>A0A8S1VTS2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WD-40 repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0730208</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000073">
        <property type="protein sequence ID" value="CAD8180201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VTS2"/>
    <dbReference type="OrthoDB" id="338608at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="218"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="336"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="412"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="689"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="731"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="773"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="815"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="857"/>
            <end position="898"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8180201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="988" mass="112794" checksum="ADCD66823D07D53C" modified="2022-10-12" version="1">MMDSFLQKEAFVDIFAQVKDVDEQIFGFIFKTFRKEKVQDCIGYLSDNGNQKQLEQQFLQKFNLALVEMEQKLYNFRNQMKQITDGLKKIKNHDFNKKDYSDEKIYQSIRQLITIMKGKRQMIEFLLFLVHLTSIDNSLIKCGSNSLHLLVQMNVDLSQKNFENIKISKTSLVRANFFRCDLSGSEFDNVNISGINLNCAKLFHCKWQNIRVDQLHHLKGHLSQVKQVSFSPNGTTLVSCDGDTSIRIWDIKRGKTLSLFYSSRITSVCFSPNNSNLATYGDYLNIWDTKRGKILSKLNYNGQVICYSPNGTTLAFNYIKLIIIWDVERRQQKFELCGHDRYVQSVCFSPEGTTLASGGEDRMIYLWDVKTGKKKFKLRCDEGEIASLFFSPDGTILYPYVEYQNKKKNFSLKGHGNYVKSLCFSPDGTTLASGGDEVFMRLWEVKTGQIKAQLYCNNSINSICYSPDGSKLSFNGVDSSILLYDSKAIEINTLLNYYWGVFIQFFPDGKTLMSYDRSLRILDVQTGKENFNQKIKLKLQMQCAFPQMELYWHRMDPYLFKYGMLKQENQNLNQMVTLNQYVQSAFLLMVLHQHLVVTISLYVSGMQKHDRVWQVCFSPDSSSLASVCDDNYIYQWNVNTGNLKSLLKGHNNFFNTITFSPDGLMLAVVSDYSFIFLMKAKTGKCLYKFEGHDGNINQICFSPDSTQLASGGYDTSILLSDVQTGQQKSQLNGHLYCVNSICFSPDGSYLASGSWDNSICIWDVKTSQQNDQLNGHSDCVSSVCFSPNGTLLASGSFDNLIILWDVKTAIQKSKFEGHTQGVNSICFYPDSTRLASGSKDLSICLWDVKTRQLISQLFGHSSSVRSVNFSPNCNILASSSDDGSICLWDMAKGQKIQDLDKFCQEIPTKFQNPIKTFSLLNNDKFVIPYTKLLISSDPISTLIYNQDQQFISGYSQKIKSFIELNFIISIFNQYIINCQNDIIFSIDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W3X5</accession>
    <name>A0A8S1W3X5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0800003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8182955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W3X5"/>
    <dbReference type="OrthoDB" id="420945at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097363">
        <property type="term" value="F:protein O-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037919">
        <property type="entry name" value="OGT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44366">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE 110 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44366:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE 110 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="23"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="57"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="91"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="125"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="159"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="193"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="227"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8182955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="284" mass="32670" checksum="FFE9FD6A9A047F63" modified="2022-10-12" version="1">MTIIKQSNLILNMLEPYLTEVNTITLNILAILYDDIGDIDKALSDYNQAIKLDPNEATAYFNKGILYTNIGDKDKALSDYNQAIKLDPKYVDAYFNRAVLYNDIGDKDKALSDYNQAIKLDPKYVDAYFNRAVLYNDIGDKDKALSDYNQAIKLDPKYVDAYFNRAILYNDIGDKDKALSDYNQAIKLDPKYVDAYFNRAILYDDIGDKDKALSDYNQAIKLDPKYVDAYFNRAILYNDIGDKDKALSDYNQAIQLDPKLVRALINRGEYYNTQYISHIVLSNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W618</accession>
    <name>A0A8S1W618_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">Fructose-bisphosphate aldolase</fullName>
            <ecNumber evidence="5 10">4.1.2.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0820040</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:14729"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.1.2.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the class I fructose-bisphosphate aldolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.13" evidence="5 10"/>
    <dbReference type="EMBL" id="CAJJDO010000082">
        <property type="protein sequence ID" value="CAD8183902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W618"/>
    <dbReference type="OrthoDB" id="36455at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051289">
        <property type="term" value="P:protein homotetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00948">
        <property type="entry name" value="FBP_aldolase_I_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000187">
        <property type="entry name" value="Fructose-bisphosphate aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029768">
        <property type="entry name" value="Aldolase_I_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000741">
        <property type="entry name" value="FBA_I"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033379">
        <property type="entry name" value="FrucBisAld_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11627">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00274">
        <property type="entry name" value="Glycolytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00158">
        <property type="entry name" value="ALDOLASE_CLASS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="8 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0704">Schiff base</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8183902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="359" mass="38935" checksum="CC46A6AE2054ED26" modified="2022-10-12" version="1">MQQKPDYRKELAETAQKICTPGKGILAADESQGTIGKKFVTINVENNEENRRAYRELLFTAPGIENYISGVILFSETVKHATKDGKNFVQLLQEKGIVAGIKVDKGLGVLPGTQDESATLGLDTLASMAAEHYKLGCRFAKWRAVLKIGNGLPSQQAIQENAWGLARYAAICQENGLVPIVEPEILADGDHSIEVCQKVTEKVLAAVFKALNENNIFLEGCLLKPNMVTAGSTNADRNKVTPQEIGYRTALALSRTVPPALVGVTFLSGGQSEEEASLNLNAMNQLTTVRKPWALTFSYGRALQNTAVKTWAGKQENWEAAQQALLTRAKANSEAQLGKYQGGQGGASNESLFVADYKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WAW9</accession>
    <name>A0A8S1WAW9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0860115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000086">
        <property type="protein sequence ID" value="CAD8186013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WAW9"/>
    <dbReference type="OrthoDB" id="307087at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="207"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8186013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="459" mass="53482" checksum="8F4754161FA7B713" modified="2022-10-12" version="1">MIQQQGQQEEVQKQETKDEGRNASSYGEEGRKGQSKRRDSSNESYSNQSSSFENKIGKRYYPHSEFKIHFYVRHVHMNGMSTNEFLSFIKRKVNLAGSCEVVQMFNTQGSYCDVAIGMSNDDNAKLVYIGKKHFNFGRNGRQKEVVELSDAFKNYLENKNMLEKEHLERSLRRNQQSKGRSDSSESSRRYKSRSVSKKKKSKKVDSKQSQGQGSRRRSPSSSSEPRRIKQVKKNRQSSRSSSLDLNKDQLNVQPIKPQQVYTDKNSVYIFSIPQEVNENDVIQEIVTHHKQNAPIHQIWNTSDKMQYLELQFQDENTAQFLVNLRPCLQVKGIPLLVVAKKQRPVQDLLRNYEVQVELDRDVPPFNVYLEFKKYGDLIGIYVFQQNRNYLLLYSSSSQLQEALRPPVHLQLVINDQNVNANAKIIDKPIDIQRPFIKETLAYQLSTQQDNKDRKRKDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WHB5</accession>
    <name>A0A8S1WHB5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0920078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000092">
        <property type="protein sequence ID" value="CAD8188763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WHB5"/>
    <dbReference type="OrthoDB" id="295118at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035797641" evidence="1">
        <location>
            <begin position="17"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8188763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="350" mass="38376" checksum="DBB2028CB4A62B66" modified="2022-10-12" version="1">MKIAFTVLVVLAISNASMMSKYPLQYGGKRSIMNIMVEVENKIKTHSPLDSIKGILNSFKSAVAQEQSGHDDVYNGQKAECDSEIAYRRLEVEDSNGTLKIANGILKTANILLKKTQATLGETENILNTVSQHISLINDARKEDTQSYNRGAVSFNDAINAIDDSIDLANALAKGEASLVQVADMTTKLMQSAIATKMNKSFMQAIGALAQITQEEDSTGAAERLGQLLQTLRNTVEEAWALYTAENTNALGLFSQQKDLYLGSQVRLDNAKTHLSSKAENLQGTISTQTAVAQAASNKRQRNQTLWDDAADLCHSFDVEYETVTAGRRQELVLVNELERLATRRAAEQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WKW4</accession>
    <name>A0A8S1WKW4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0970135</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000097">
        <property type="protein sequence ID" value="CAD8190824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="62528at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010441">
        <property type="entry name" value="CH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052634">
        <property type="entry name" value="Sperm_flagellar-bone_growth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14919">
        <property type="entry name" value="KPL2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14919:SF0">
        <property type="entry name" value="SPERM FLAGELLAR PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06294">
        <property type="entry name" value="CH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="2">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="184"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="224"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8190824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="347" mass="39882" checksum="CE36DFE87F4AB85E" modified="2022-10-12" version="1">MSQLLMVWLNDEVQLSRKVTSFEKDFNNGYLFGELLSKFNQQLNFEEFSNKDVREAKMKNFTLLEPTFKTLHIPFNFQIADQVIKGKKGVAMQMLYQLQMQLQKVNDPHDVMMHAKTGKYNVIQPLMVIRQPKEQFDKMEQEFFVSKLNEKNKAQKDVXFQIFRGADNKIFVLNVEKGKKELEIKIHKGQVNSLKLIKDILISGSSDHTIKYYNLEEQKEISVISGHQNTITSIAVTPDCKMLISGSDDISIGVWDMMTQKQLASLQTSDQVKCIDICPNGQIFAAGCYDGSIHLYKIIDNQKFDCYKTFSMCPLIQARDCILKNSKIQSRTDSLEPLLIQKGAIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WMF8</accession>
    <name>A0A8S1WMF8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0940218</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WMF8"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000096">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF13">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF19 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 6 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="10">
        <location>
            <begin position="21"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="583"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="670"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="371"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="487"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="583"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="670"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="115"/>
            <end position="122"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8189801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="803" mass="92797" checksum="000DA0CC5675EEBE" modified="2022-10-12" version="1">MNQFEIETKKFIDELKNGVSNILVAIRVRPLSQKEKSLSEFETIKILDNKMIVLMDPEQERDEDLLRKNRLKETNFAFDFVFDQWASQQMIYENTTEFLLEGVFEGYNTTVFCYGATGSGKTFTMIGTQLEVGLMPRALQQLFNFSIQDRFKNTQFKVCYVEIYNENIRDLLTSEDKNLEIREDKNNGIQIAGVTEVEVKTVSEVLALLKVGNKNRSKEATDANKESSRSHAILQLQVESKDRAAGLHEQIIQSKFSLVDLAGSERAANTNNKGQRMIEGANINKSLLVLGNCIQSLSEANEKGIKNPFIPFRNSKLTRLLKDSLGGNCRTVMISNVTSAVSCFEETYNTLVYANRAKNIKTVANRNVLMAQNHISNYAQLIQNLRQENEELKLLIQQQQYSQNTKQLKLPSINQKNHPVPQLSLKQQAKQLESIINQNIEDITETKNKIYEMEEQLNHFQQNIGFLQFQKGRTQDKFEQMRLQERMDNAKTQKAIIKRSQDDLEQQLLEYEMQKVDIQKQIQQIQDSNYKNYLNGIMKQGDFKIETVEIKILEKKRKYQEQIQDEQVKQLRTQINKQQAKIVQSTKQKSNGSKKIPSLPGVDSPYYQLQGGQTYSIQRQTMFINRYSKNKSHLKLPPVVYINQVSKSPNTQHANKMNDPMKYRVAQKYSSSLNRPPSFHPPSSNSKSEKGKYINRSLDLGSGRDSINKNNGDLQNDISLRKLHRLRQEYQQQRFEKAMNNKSNQKSVPSFGSKILLPGMIHMSPYVRNFQNNIEPIELKKERLKMLNTNQKGQVGDKFQLYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WN72</accession>
    <name>A0A8S1WN72_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0940142</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WN72"/>
    <dbReference type="OrthoDB" id="282211at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF179">
        <property type="entry name" value="OS10G0565000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8189669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="208" mass="24362" checksum="1A620D68A92E9C3A" modified="2022-10-12" version="1">MYYNYYSRIHYVQPNQPQQCCNPYIVSPRMSVIPTSIKQMRESIQIPYNQDIMKASAILEVGQRTRNTHYKQRLEELYLKYEDGCIYRGQGIPPSQRHGMGVLFNTENQEVYSGEWFNNLYEGFGILINQSPHVIKHKIDPKDLNTIEDGWHDYKGYFKQNKMHGEGILTLKNGQKFEGHFINGMIHGKGRFYGDQIVVGNWYEGVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WNK6</accession>
    <name>A0A8S1WNK6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0990112</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000099">
        <property type="protein sequence ID" value="CAD8191584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WNK6"/>
    <dbReference type="OrthoDB" id="294049at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="460"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8191584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="550" mass="64005" checksum="9F19740458D337E1" modified="2022-10-12" version="1">MFIFIYLIINVQSYRIRQIQNESLEFQIPTEEETKLLTLFQAKDAKLITNETIPFCQLKEINLIFETKRQHFENIVTMVHVRDGVIFITSDYLLYLMKFNYQNFSGEFAKIAWKADFKELGLDTLAEMPQLLYCSTSNLGILFQTRGAILFSVQQMEQKAAKLQIRDLSEIKHRKERGVTKEYDNYIFSCVGQDGLDLYKIIGNELHFIDQIGNTRFKDLAILKIDNTIYLILLDEQQQSISVYQMDIVKIKISLKLQNKFQSINLEFIAIDNYKNNIFVVYQYHHKYICVEYLFTEKELIELNSFETFSKIKDVDVSQQFAILQGQNKHHIMFVTKFDGLQISHIPQYTLLGAIDIDFFYLTNIDLAMSHLIDQNDFSIENFFFGTSKSGLFFTKFKFQDPYIICNPQSNQKNQQQNYSIIQNESVIIQTNIQNNVILQRQTNITIMVFDEDDNTYSQIILYLGIPIGLSILLIGICFWFISKNQEIERLETEITAIKSKKSSGLAVAVEFNTPQIIQIINSPIIPSPFSNTERANVNTNLNRDINADG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WP99</accession>
    <name>A0A8S1WP99_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Calcium-dependent protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0960132</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000096">
        <property type="protein sequence ID" value="CAD8190461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WP99"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000771">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000660">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="11"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="297"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="333"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="548"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="584"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="369"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="477"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="369"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="391"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="418"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="501"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="514"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8190461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="637" mass="74583" checksum="8294970A4498FA7C" modified="2022-10-12" version="1">MGEKRVDRFVFKISDKLGEGSYGQVFKGQDEKTKEFVAIKMLSKAVINEDEYLREGLMNEIKIMGTLQGNNVVKLIDVLETSNNYYIVQEYCDGGDLQHLLKKKKYLPQKEAMQFFVDVLLGMIELVKKGIIHRDLKPANILINKGIYKIADFGFSKAIDNFRKQMIESIVGTPLYQSLQLLKGEKYTSKSDIWSLGFIFYETLFGSTPWTARSIPELVKNISSQPLKFPQDKNIDPQIIDMLKGCLQLQEKDRMGWDLLYRHPCFGQSFSFYTNQTKQLEDKAVFLVQDLRFKVMKDKIDLEKVFNKYDKSGDRSLDMKELTLLLHEIDPKLEREEIEFMFNKIDLDGSNSIELNEFKKWLEENQVQMSMRNQPKSNFSRKGTVEMPKVNPNNSDQPVPEFDSTQMSTNTTNLTPSQHQQQQIINQQQYQKQYSNQQSLQPSQQLQQPIQQPQQFQQYPQQQQQYYSQQYQYQYPPVQQPMSGSQHQQNYPIQPPPSYPPMNNSQQYNQGYQQPPPYVAIHQPPPPQSLAQERAMVTIQKLVIAIEKYNINIFELFRKYDKSDSQTLDVKEFGVMLRKIDSQLTDQDISQAFWVFDVDRSHEITFKEFQDGILLHLNQWRAQANIQNNAGYPYQKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WPV0</accession>
    <name>A0A8S1WPV0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0980158</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000098">
        <property type="protein sequence ID" value="CAD8191252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="298642at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00198">
        <property type="entry name" value="vWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040395">
        <property type="entry name" value="TTC19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13143">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13143:SF6">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 19, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="446"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="790"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="790"/>
            <end position="807"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8191252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1304" mass="152321" checksum="6FB2BB578DC5D5B2" modified="2022-10-12" version="1">MELKNQILLVILIPIILILILAVLQVGLLGFVSIPSFQDSLLEFHINRHLNGLQIEMNIIQQEIYIQYQAYYNQIFGVSAHLRHYCFGEFQNFSSWTLNTKQIAPYSFSSGQITEEYYTVAWSHSNYSKLEALHKGNPRLSFLINKITQSLILSDIIINNTRSSSQDKQLVSKDREDDSRLIPITYDSIMVHCTDSLVVRIPQFTQKQQNLLKNINTADWLPKERFKLDSTLNFSMSYVDKDEFTQNPSLTITQIISQDNFNQCFMTAKISNSYLEDLIKRHHQQHKIQGESEHQFFIAIINDEFINLAPQDFSNIPISESIIKYIVKTKDFSQSSYNSLNKDLAPQIMNKSKELVQNATLNSTSLTYSFGQNKFYLLFSKVNIYSTKKVSIVTAPENGRRYIYIEPEENNFIMPSFILVHYFEEQTLIQELYEGTSLNNVRMLEIIVFIVIIALSVVIFIFVWYTAGRIGLSFEQPIKVLTDFMNSIDIQNMDQEEELINYQDYFNSFEIKSLFQTMNIFVTTIKYSNQKYSNCKHSDALALMELSKAKDFYKKEIGNMSAVGICANNIGILHMKGGRVFEAINEMEEAIYIAKLELIEIKELKKWCLGMQSALQEPNLYQNFKQRLKGLAQKFKEQMKINWIGVQINKNNKKTKKQNSQLKLNSQSLVNKPKEKHNKNQSTTQLLQKNSIPHVALLNTGTQNKITQNQTNSIQTPINKKRMSFAESRQHMQPINSVEIENSPNITPQLSPNVSINRLRNLGSHSYNQSSIISQRLDLKESIIQQDERYRDEEYRQDQEERIRGDSRQSTGGMTELQMIEEKITELMKKKKLAKVKLLNRQFQLAQFMFTICMQNEIFIPETLKLFNEFEATAEKEQQFTQSNIVRLLNLHVKKALCYIQIGDLSNFKISQKKALEYYNKLNENFTQEQNQIQEDYNFVDMFKNVPKEVLLSKIMQLQAIYELIEGNFKTGSEILTQIIELGEYYDPEVRDFCLTALEKIINLFSISPLPIQQFRNQISINTYEIVFMIDYSKEMTIEQINLSHSICNRIFHILQPQDLIGMYGFNNSLHEAFPLQPKGTYKDLLTKQLFMAITAPGGKSKIFQALKYAVKNFFEHKITINDNDYDKKQNFNLNDKQKMQLIPVAEEDEEKSLIEENIDDKSKHVNFSQEYQYDLNSESDSEIENDDFQFIKNEIDYSNIIENDKSQQKNDFRTSYKFICIFTEINNKMSEKQFLKLRQILEKNQVDLMVFNIANQNANMLELKRLSKITKRSIFVNATANLEQLFGKSRQHQLQKKMYLEFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WUT2</accession>
    <name>A0A8S1WUT2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1020034</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000102">
        <property type="protein sequence ID" value="CAD8192547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WUT2"/>
    <dbReference type="OrthoDB" id="308265at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="187"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8192547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="386" mass="46375" checksum="269206AC6CDF2667" modified="2022-10-12" version="1">MQQKQDIQFELIEIQSLVNQGRQQEALKQCDIAISENLNEVELYTIKTKILESLGLQNEVIDIWEQGIQNNKNDFLFYYEKVFALRRQLKYEEIIKCYDQGIMNNKNQAIYYNFKYIELEKQDRLVEAIQVWDLAIENNKTDSYYYRQKIQALIKCFGEDNCQKQIFECWDYGIQNGNNNLFFIKSKVKALEKLERLDEAIVFLSKEIQQYKNVEFLYEQKTNYLKKLNRLEEIIECWNEGIHYNPYNKCFYQQRIKALEEQGNIEQVLEEANKCIDLFDDLIFYQAKAKCLFIQGKFQEVIDCWDQGIKNNQSNSSFYIEKNYALQLQKRWIDSIQCCDSALLNEYNNEEIISKKVISLIELHNFRQANQYHRLIHLNVPIPFKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WUV7</accession>
    <name>A0A8S1WUV7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1020016</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000102">
        <property type="protein sequence ID" value="CAD8192521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="29013at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13174">
        <property type="entry name" value="TPR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="278"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="345"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="215"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="624"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="692"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="811"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="845"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="879"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="913"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="947"/>
            <end position="980"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8192521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1036" mass="124266" checksum="32B6589065DEB285" modified="2022-10-12" version="1">MKQNSQFQQSQDFKCRDHPENLALFWCRSKDCNENRIFCLNCQKQNKHIQHYNEDVLSIHELTQFLINQSRQPQNLIEECKLQQQSTIKSFDKLISGLSYKFCGIEDKLNQFNHYQIQQALDSLIKFDEFKNHMKNNILGILKKYQKILDDLFIKLELHLIQYQITDEQIEFNKQEQQKGISLNKINKQNEAIQLFDKLLLIEPYNIEIMYQKGQQLLLIQANSLIQLNKLQDAQQIYQEIISINPKHEISLQKLGDILLNQKFYLQAQKYYEKCISINLNNSIVQFGIGFIFINYLKGXSSLQCWICFISISTIIYLIVALAYRIVQNFQHLIFLQSIQKQNMIYYVSRFFSILLQAQRIIGIYIFQEIDDIPSLQQIWPIEAIRKQYQTLSRMNCIQLRQFQRNTLQIDFLQFIIMKQNSQFQQSQDFKCRDHPENLALFWCRSKDCNENRIFCLNCQKQNKHIQHYNEDVLSIHELTQFLINQSRQPQNLIEECKLQQQSTIKSFDKLISGLSYKFCGIEDKLNQFNHYQIQQALDSLIKFDEFKNHMKNNILGILKKYQKILDDLFIKLELHLIQYQITDEQIEFNKQEQQKGISLNKINKQNEAIQLFDKLLLIEPYNIEIMYQKANSLFQLNKLEDAQQIYQDIITINPQYEFSLLKIGDILRHQNSYYQAQDYYGKCISINQNNQEAYFGIGECLRQTYQYNHALSFYQKAIELDQSNYKYYFYYGLIQSLNFQEIVQDLYKDIKKLSIFMIKSQLKIKIKQRQWQEKEYVQYFQDRLMKHMQLFQSHLIYKIILYQDYMDTVNFKQQILGIYLLSNNRNEEAFKQFDKALSINPQHDDSLWGKGVCLRMMSYFQQALEWFEKALAINPQHIYSLWGKGDYLRMTSQFEQALEWYEKALAINKQHFDSLWGKGDCLRMIDQFEQALEWYQKALTINPQHVYSLWGKGDCLRMIDQFEQALEWYEKALAINPQHVYSLWGKGRWSQIILLAFTLLKFQQFDDAYFIFKLAKQILPNQEFIDAFLEQFSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WV16</accession>
    <name>A0A8S1WV16_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T1020051</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
            <location evidence="1">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000102">
        <property type="protein sequence ID" value="CAD8192581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WV16"/>
    <dbReference type="OrthoDB" id="294378at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46613">
        <property type="entry name" value="RADIAL SPOKE HEAD 10 HOMOLOG B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46613:SF1">
        <property type="entry name" value="RADIAL SPOKE HEAD 10 HOMOLOG B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0282">Flagellum</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="297"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="680"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="767"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="814"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="316"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="366"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="693"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="814"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="839"/>
            <end position="850"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8192581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="850" mass="99398" checksum="4FF15605A8ECF8DD" modified="2022-10-12" version="1">MNFKEDPKNLIIPLDLAKIIYDKWDADLAILDQPDQVYGKALHGKGEVTFKNGNKYEGEFHNGMLHGEGTFTWASGVIYKGEFTYNKIEGQGTYYWPEGSTYTGIVVNGLRHGQGKFVTADKSAVYEGQWENGLRHGFGKITFQSGATFEGQFYQGNKSGKGKMIYPSGNYYDGDFLMDKKEGQGVMFWLNSNEKYYGEWKDNVQNGWGVHLWIEPKGEGSKYLRNRYEGEWENGERSGVGVFYYANGAKYMGSWKNNLKYGVALFITDTGGFILGEFKQDRLIKVYATTENESRTGILPREIAPQGGEVIQQNENPKEVGDNKDRKKDNKDNNKDKDNKDNKDKDKDKNKLGNTTKRPEKLAGINNPNQTQTNLNKTIETNPYSYMIDFSDLYKGLQLDSQQIDQNVQTMLLRHNYTLKTHYKFFANKFESLLYEDTFSLSLEGFWKIARDAKLLNQNYSLAELNRNFLQGSKNNYRLKYDRQELNVEIDVLSRQGSNINLQELNDLFHQQMAFDCLSTEKINYQDFQLKDVTFKKKVEDIHDPRRCILFRHFIELIVRLSYLKYGNLIDLHRAIERVIISKLTPYFERKKGKQNQSLDDTKKSTHPKIIIGDFIDEVKPIFDVWSIENRETQFGFTDRTINLEQIYKLLNASEQDKLILISYVDKNFDTDEGQITQQSISQIDQSSVNQTIREDGNKRRKKQQDQTAEKDQRKTLEKSTSIIKYELLLFEFTDILTSFLVKKHKDLHTNKEDEFKIRMSNHIKKLKNRKPPTLNKYKQPRLEPQTQKDIQKEQLKQLRIQQEIKARQRKLLEEERKQEERERNLMALEDNDIKSEHEDDDVESEESDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WXF0</accession>
    <name>A0A8S1WXF0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1070130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000107">
        <property type="protein sequence ID" value="CAD8194724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WXF0"/>
    <dbReference type="OrthoDB" id="304552at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8194724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="221" mass="26724" checksum="7D675207130B1968" modified="2022-10-12" version="1">MDNYFTIISLLGLRNQNLPPFREARLKRYKSIKKMVELIETAGWTQPKIPFNAFCLSSQDPEWEDDMTYPVIEYNKFGYQAVAFGINLFLYAYNYNVITQNIRFRTFRYLFPVVQCVIFGKIYFEYKSELTKVNLFDEYVQLRAQELVKENEFLLEHEDIKRFVWWYEDYKETLCRVHRQANDHAATDFKDSELILQDFIRRYTNPNSPRPLNYQEKGVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X2T6</accession>
    <name>A0A8S1X2T6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1120141</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000112">
        <property type="protein sequence ID" value="CAD8196464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X2T6"/>
    <dbReference type="OrthoDB" id="292148at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8196464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="252" mass="30733" checksum="89DA352DAB5034F7" modified="2022-10-12" version="1">MKEEDNNIKQEENQFFIPHYIIPINYAPIPNEIENYKMAIHHLTEENHFLRKQLQHNQVSEFLSLNAEQLKDEVFKMIDYLMKNLNYLPKEQIFAYRKTIRLCTQRSALKRIYFLIFTRYLQAEKTKEEMIKFIIRKSVKYQKSTSTIQKHQMKKMNNAFVQQLFLSTQYQQHYSNFLNQYLQLALDENKHKIQKYVDYIVEQISNDQIDDVLNYKRFPWLNEWIQQSVQIAKDLQFSSKPQSLENKKKIKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X3L0</accession>
    <name>A0A8S1X3L0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SANT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1100010</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000110">
        <property type="protein sequence ID" value="CAD8195561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X3L0"/>
    <dbReference type="OrthoDB" id="2193595at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000012">
        <property type="entry name" value="Metastasis-associated 1 family, member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SANT" evidence="7">
        <location>
            <begin position="227"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="171"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="190"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8195561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="297" mass="35011" checksum="607F493E5118F0B1" modified="2022-10-12" version="1">MKNQYDEQQKIKNNSTQFEDNNGIDLGIRKVIKLGENEDKIATNLQYESLRDTEQQKQVIQQIKHLARQGSHITGYLELPTEVINQYKKKSNSDALIDEVLLDKFLPPNSITPLIFTKKLPQGGQYRQKGTQPLWVNAFESLLSVVVQLYYKSDQKLAEFMKEKVQIDSKKDIKQEKKPGRKKKILDNDSPNQEQIEQQQPNSQNVYSLLQQDVLDILISPLRNDFSFETWTTKEIAIFECGLCRYGKQYEFLSHLIKTKNAQDIIQFYYNWKFTSHYKLWKINKAYYHRSNLNNYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X4X3</accession>
    <name>A0A8S1X4X3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1100049</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000110">
        <property type="protein sequence ID" value="CAD8195639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X4X3"/>
    <dbReference type="OrthoDB" id="308931at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="165"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8195639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="254" mass="29568" checksum="FCB6D93C0617D19A" modified="2022-10-12" version="1">MIPVQILKNIQNQNTQHRQTNSAFLKSRKHSPITHLIVTRHPRVSQQMKEQSNSPSTKLKTSTQYPQIIQSVEQMKRFPTEANVKSIKGSTIQISNALKLFSSKHPSDQQQNTLKYSSFIYEKVNEQSIYTKTTTGESQENDKVFNQGKVLNGNLDSFIVYNNVLHCKNLEIQKLEKELKQLKQENRKYQNLEKDYQTILQENQNLKLKVQNQFTEINLLNHQQTCNSLRKQSDYVSALSDQDKLKIKTLLECD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X5Q7</accession>
    <name>A0A8S1X5Q7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1110163</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000111">
        <property type="protein sequence ID" value="CAD8196165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X5Q7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8196165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="386" mass="47094" checksum="5CACA57E86AAC5CC" modified="2022-10-12" version="1">MNCLTNNEDYPFDNNQLLSFCYVVYYKLYFYNRDFNFILQITAFLAFIIQTLNYQYKSLAEQGYKVFERDKCYKIEQRQWIGRYRDQLKNLNVLHGIQVIGQERQLWPNSRNDQIFMKKMMEFWCVDFHLNRSKWNLDMIIWFEWDRELKINRNEQQFQEISSIIFLYQVRLLTCVGLVTQYDSKISKFNLKKIILVLQRILAKIQEHLKHEQEKKKTNEYFFSRKGTYQLQYQLKIAFFSNPKYQNISKYLTIFSRIFFLFLRRFSKFLISKIFFSNPQHTNLNLQKGDLNLKIKIFLRAKIKLNQVSLLNELSEQSLQIPSQNSSFYITQWLTLLHKIKLTDGLSEPIKHFKHLNTFMIRLGKIIVPKGIQFSKRRYWILFEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X8T5</accession>
    <name>A0A8S1X8T5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T1150091</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000115">
        <property type="protein sequence ID" value="CAD8197408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X8T5"/>
    <dbReference type="OrthoDB" id="354826at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05578">
        <property type="entry name" value="STKc_Yank1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000454">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000919">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF374">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="23"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="11">
        <location>
            <begin position="282"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="301"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8197408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="351" mass="40082" checksum="110D09BE7F9FD616" modified="2022-10-12" version="1">MGNCNITDKKDLPDSAILSVHNFEFIEVLGKGGFGKVWKVKRRKNKSYFALKVMSKAKVISKKSVSSVMNERTLLSQLRHPSLINMIAAFQDRENLYLVMDLLSGGDLRYHIGKNKKFSEEETKFFCACIIIALEYLHSQGIIHRDLKPENLVFDSEGYLRLTDLGIARIWRPDNSSDTSGTPGYMAPEVLCRQNHGIAVDYFALGVIVYECMLGRRPYVGKSRQEIRDNVLAKQVQIKRNEMPVSWSLEAGDFANQLIQRKPVQRLGSDNPEAVKNHPWFKGFEWNKLLNKELHPPYIPRSSSNQGGSTQDTEQDTNENNMLLRRNSIQAQFDGYNYDPNEAMIAEPSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X9N6</accession>
    <name>A0A8S1X9N6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RNA polymerase subunit H/Rpb5 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1160112</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeal Rpo5/eukaryotic RPB5 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000116">
        <property type="protein sequence ID" value="CAD8197786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X9N6"/>
    <dbReference type="OrthoDB" id="248779at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.940.20:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00025">
        <property type="entry name" value="RNApol_Rpo5_RPB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014381">
        <property type="entry name" value="Arch_Rpo5/euc_Rpb5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000783">
        <property type="entry name" value="RNA_pol_subH/Rpb5_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007129">
        <property type="entry name" value="PRK09570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01191">
        <property type="entry name" value="RNA_pol_Rpb5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000747">
        <property type="entry name" value="RPB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase subunit H/Rpb5 C-terminal" evidence="4">
        <location>
            <begin position="151"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8197786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="224" mass="26472" checksum="B26E8E8138A25FF5" modified="2022-10-12" version="1">MIEEEYRPEDKASIKKKYKVFQTLASMIRQRGYFEAHPVILVEDEPFELFEARYRTDTLPQKIVCFKRDDETEKLVIIFYTKQQALSHEDANKYIIEFTNRQVKRLVMIFPTDPFATKPQIVEKKARLFIDQVNSIQTYSFELFGEDDLVYDVTKHELVPQHIILNDKEKEQLQKKYGLVDNQLPRILQSDPIARFLGLHHGDVVKIIRKSETAGLYVTYRIAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XBM3</accession>
    <name>A0A8S1XBM3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1180014</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000118">
        <property type="protein sequence ID" value="CAD8198212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XBM3"/>
    <dbReference type="OrthoDB" id="71437at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002639">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="366"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="92"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8198212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="604" mass="70054" checksum="66A0513757EA7A6D" modified="2022-10-12" version="1">MLKPQVIESQQNLICMNKHDLPISQVVWDPKLNYDQRLLCTQCVEDYESETRTMGYKKVFQIIEEKQRMKIQEYENLLMPHINSVLQFQTAINTLKQNIIQQLDQLQRISEDWIKNLESTKKEISKYSFFQELDKLIKNEQNHFDENKHKKEIQLIHTEWSNQLTEKLDLFKNFQQYNDCLEILKKINQVEESLQLSPPLSPIKLLSERFSIKLPTQSKSSNQLSNLLGNSQISNSPFTYQLLPQMSHKQTYFCRAIAINHDNSLIIVACNSNIKIMEILLDSQLQLSSSTINSINEIEIKKTPKLLQLLQGEHQDFVNTLNFFRNTPSMLNSFLSGSKDSTIIIWSPTNFSSQLQPILWGPLFKLNGHSNSIRCLIIHPLSEDLVISGSDDSSIKFWSCATFFQQKCQWTCQQTINEHLDCVWGLSLNEDGNKLISCGEDKQILVMELSNKQQWEIKQQIQVEIQGMRICFINNNQFSFQACSGKALPNLHIYTLNPSSGLFSKSKDILVYGGGQPCVFYFPQQYIPSKQILISKNGCYVNLIRFQFSSFDEIQESQLEQSIHFGIECDGEIFGTISEDGEFLITWDSKSKDVQIRQYIERKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XEP7</accession>
    <name>A0A8S1XEP7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T1210117</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Plays a fundamental role in microtubule organizing center structure and function. Component of the infraciliary lattice (ICL) and the ciliary basal bodies.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the centrin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000121">
        <property type="protein sequence ID" value="CAD8199313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XEP7"/>
    <dbReference type="OrthoDB" id="343296at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000178">
        <property type="entry name" value="Calmodulin-2 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF59">
        <property type="entry name" value="EF-HAND SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="15"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="51"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="89"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="125"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8199313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="160" mass="18511" checksum="C000FB3485225100" modified="2022-10-12" version="1">MQNNKKAKRTKLTKEQIDVLQQAFTLFDTDKSGSIDESELRNAMKALGFNASKEEVQKMVEQIDRDGSGTIEFQEFVEMMKKKMLEDKNVEVEIEKAFNYFDDDNEGAIDLEKLRRVAADLGEECDEQTLKDMIYAADLDQDGKVSKDEFMMVMRKMKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XR09</accession>
    <name>A0A8S1XR09_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Branched-chain-amino-acid aminotransferase</fullName>
            <ecNumber evidence="8">2.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1320154</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:18321"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24813"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.42" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDO010000132">
        <property type="protein sequence ID" value="CAD8203002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XR09"/>
    <dbReference type="OrthoDB" id="1732691at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004084">
        <property type="term" value="F:branched-chain-amino-acid transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009082">
        <property type="term" value="P:branched-chain amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01557">
        <property type="entry name" value="BCAT_beta_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.10:FF:000017">
        <property type="entry name" value="Branched-chain amino acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.10.10:FF:000027">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018300">
        <property type="entry name" value="Aminotrans_IV_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005786">
        <property type="entry name" value="B_amino_transII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033939">
        <property type="entry name" value="BCAT_family"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01123">
        <property type="entry name" value="ilvE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009897">
        <property type="entry name" value="PRK13357.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11825:SF44">
        <property type="entry name" value="BRANCHED-CHAIN-AMINO-ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11825">
        <property type="entry name" value="SUBGROUP IIII AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006468">
        <property type="entry name" value="BCAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00770">
        <property type="entry name" value="AA_TRANSFER_CLASS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5 8" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="4 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8203002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="390" mass="44314" checksum="D8ED4D89919DA1F5" modified="2022-10-12" version="1">MSASKVQKLFFRFSYYKNLDLSSPYPSFQARDMTTTHTMHYITKPPCDPKIMTFGAFHTDHMLEVDWTEKMGWSRPQIIPFKSFSIHPFAACLHYAIECFEGAKAYKGPNKTIRTFRLDCNMYRMKQSAKRLSLPDFDGAELQKCIEGLLKVDQDWIPDRPGFSCYIRPTIIATEEALGVRASSRAKLFVVLCPVGPYFPSGLKPVRVFCNTTTIRSAPGGVGGYKVAGNYAPTVMPLKEVQQIGFHQNLWMLPDGLVQEMGVCNLFFFWTNKQGERELVTPQLDGTILPGIVRDSLLELTREMKQFKVIEKKLYIQEVIEAISEGRMIEMFGSGTAVSIQPIEAIGYNDKIYEVKYDPKLNAGELSHKLFDMITEIQTGGQPHKWINVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XR65</accession>
    <name>A0A8S1XR65_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1340070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000134">
        <property type="protein sequence ID" value="CAD8203580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XR65"/>
    <dbReference type="OrthoDB" id="294977at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8203580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="504" mass="59381" checksum="208EB1DF31EFEFFC" modified="2022-10-12" version="1">MQQKSINLIHRKFKFPEIESSNKSNRSRGSPTSSRNNTSQERDISIDGGNSIQVFKIRNVQVSERSLSPKEEKIFNMSKSTQLKSRQEQNEDYTDRNSQELMLPKIYSPILTPNKNIVVTKMKDSIFKPLLCRQEIIDHQINIKLQNKIHDQLKKGVNIKCLYLSQRTDVQINKIIVLNFDSLVLGKRKSFWEQQNEITMCSQIIGSKCDNSYCACALQKELKSTLSILSKQFYIVLLFECTIRGQVWQQFLIESRFSIDAIYCIQQIRLNGGGIKMKQIISNFGNSDIKQLLYFGTIDCESQYIMIPKEQFYYQIPIIQAKTNVLIYLFQLQKSKFLDSKLLFEICMLNCNKSESFNYKRPITIINYDLELIIKQMIYNEEEDIIQNYIYKNLKQEDDSVYEIKEDQEEGNDQKNLLLIWLYQAKKLLLDYFKSINNEDQIQNPISMLGDVLRRNTSIQLNFISENYEKQTKRYKLYNELKSLAIKQPSFRKQIQIDCLIIYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XUB6</accession>
    <name>A0A8S1XUB6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1360052</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000136">
        <property type="protein sequence ID" value="CAD8204148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XUB6"/>
    <dbReference type="OrthoDB" id="315653at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8204148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="696" mass="82925" checksum="88DB7E19CFB51242" modified="2022-10-12" version="1">MIFRLLYSITRTKLSSNIDFKKRCLNTLELQFDKVDDIIKSTPTLFLRGGGCVGSKAKIKSQMQNTIPMNYLQTMKLNSQLIVDKCDQLLDNFSRNEIMMSLQWFFYNRYWICEICKNDNLILMLYDLALNQFRQLISFISVYLRGSSCLCYYVLEVSNDLLMIIYTYQLKDEQRVLLVEVHDELLSMIDIIKQDLEVYQNFWTSGLDFQITLIKIVLINSRTNSKEQQDKLISIESSAFSSLLSLSISEDFTNALFDFAKYLLIEKYNQFSYPIQTYQIYYFFFLLKWSIIKNIQEQNLVNKQIANLQLGYQQYVQNSDNWIVHFCWINAISDLISYRQIISKTIFLKNQDDMKQKQLWNQLIKQNIIQILPYDKYLGKLNNSCSFKSIDYETEQILNGLDFTFQKLKKKEEKNNLPPRFELFGVDEQVKKLQWLKQLLQVIKDKILEQYIESQVESFQLPSQDVQTKNNGYNYDLVLNKEGEVQQLEFSLSKLSYLLHEVDFTMIVEKKRIEICQEQVKLYENQIKKISKIRIKKIKINQEFNKISQKIFIMSGSNQIFQINTIQFQIKLQNQEDFNSNFLNLKNQNPKNNQNKIKRMIEIYLFGMLRFKQKSFYKNLIFNNNYYRTFGGLTISQHSQQIMNNKIKKQVNSQLISLKSLKGLKYSLLIMNTIKNLRNFLFNRNFKLLKIYQNLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XUK2</accession>
    <name>A0A8S1XUK2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1370003</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000137">
        <property type="protein sequence ID" value="CAD8204322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XUK2"/>
    <dbReference type="OrthoDB" id="309408at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="2">
        <location>
            <begin position="584"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="491"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8204322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="769" mass="90217" checksum="EB3606EEDABF9741" modified="2022-10-12" version="1">MSSDLDKKLCQKHKLEILTIDLKQSTAQDDKYLCIKCRMEKIDIQNMALVEETKTMIKQMKSEQYNNNIKEYQGRIENFRQIQSQVKEYKLLINKTIDKVQSNIDQRITLMENELDESQSQTLISTFEEDVKILSQNYKGSFSFEVPKELEKFLDDNSYIDSIQQQLQSIINCPKLTQIKEALEKAKVENGHTEIKQVQLLSKKEEDPLKTPSLKIQCNKHGREIIMFNLNPEKIQFSRLACVECIQSNDPIKYTTLEDASFRWNEYLGQTSDQVKRFQNQRCLKSSQIIEILQEIQEKYNSSISEIINKVKIEYQMYSLNEINEFNENMIFEMSTEQVNELTEILSKKDKFQVLAEKQLNIQKEDLNQLEIISNNFGKLLQNDLLATERINKIFKDFSSNFYNVKEWKIDTLKEDNPISNHLQMKQLNLQLQHFILYQDILNEALNQYNLIILTINSLSNEFKDIQLQQFKQYVSKFEKDLSLMHKFSQFDQIEAELKLHKQEKESLKNQLTESEKINLGNQQIINDFKKNDAEQIKLIQDLRKQNEGQSIKIKELEQQKTQCEVIVKEKQEELQICQQQNQKQEQMLKQLQQTLVQDQQFSQSLTFSTIYKNSNCSVSQNGKVIETSYNGWQCCMCDQMIPKNGVVQFAIKIIENSNIMIGIGFRDIVQSKNYSCCHGLGGGTYNIYYNGQCYNHDQQDKSGQQIAFPFSTNDIIIVEVDIQKKQVKWKKQSTNQSFTLNIDTSKELYPCVHLQGRCKVEILNQGFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XXA6</accession>
    <name>A0A8S1XXA6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1420066</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SPF27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000142">
        <property type="protein sequence ID" value="CAD8205920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XXA6"/>
    <dbReference type="OrthoDB" id="205794at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008409">
        <property type="entry name" value="SPF27"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13296">
        <property type="entry name" value="BCAS2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13296:SF0">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR SPF27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05700">
        <property type="entry name" value="BCAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="132"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8205920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="217" mass="25618" checksum="1200A1FBD635DFE9" modified="2022-10-12" version="1">MSFALPSSKLPLVYRHPQQGFVNTLPYIDGELDNKTQTIVDRMIQQEMQNMDPQDYLHDLPMPQTKLTDLLKSEIERVQQPISKIDFEQKSNINQEFQSTHEIQEANQQLNVLNQYSQINIINSELLNKYGKESWSLLLKSQENEKNRLSNEIVDQQQELNHINAQRKYEQNEVKYKLDSLKNKVQDVLKNNCQLEVVCGELEQEIYDKQRKQLKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XY50</accession>
    <name>A0A8S1XY50_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cdc23 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1440066</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000144">
        <property type="protein sequence ID" value="CAD8206503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XY50"/>
    <dbReference type="OrthoDB" id="659at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045842">
        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007192">
        <property type="entry name" value="APC8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558">
        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558:SF10">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04049">
        <property type="entry name" value="ANAPC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 4" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1012"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1059"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1085"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="domain" description="Cdc23" evidence="7">
        <location>
            <begin position="9"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="250"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="318"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="352"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="386"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="454"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1174"/>
            <end position="1201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8206503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1246" mass="146294" checksum="B486E2DE47D41D66" modified="2022-10-12" version="1">MELMPKLQEIQKELQYYTRFFSENKLYHSSKWTGELLLGLTQEEDLQSSQFAMQFIQNNTDYNYHFIREFVPEYNDIILVARNLYDLREYKKCASLLQNLIHKNESAMFLYYYSQYMHGELRKEEEMFENENSKTAINPELKLLERELSKLYNQKQLNSLNLYLYGLILKDTMRLREAREVFIQVLHQLPCFWSVWLELCKLLTEEDTLDELPNHWMKQFWSSNFNLEKFKNANCVEQFQTLLYYFRNSNFIINQIANAYYNNQEFELSLEWFERLLSIDPYRFESLDTYSNILYIKENQGELANLALQSFTNNKYVPETCCVVGNYYSLMNEHAKAINYFQRALKLDKDCLAAWTLMGHEYLEMKNVASAIQSYRNAVEIDPKDFRAWYGLGQTYALQGMNQYALYYFSRAVISRPKDARMWNAMAECYDKMDKKNESMKCYERANQCKDKEGIAIHQLAKLYDAVGKTEKALSAFEESLKRKDDEQIVDKELSESLVYLARAFLRRGDKERAMQMAKRLYDFNGPERDEANQIMIPITLDCRYFKEALSEISNEQQFLQIEDYLLNQDLIDRVNRRTVNLMIECIKILRKYMFIGWNVSPKFIEDIVNELIDKSTEEWTEPIFFVQRLPEIRIQIKAYYKTFQENAFELCDTLIGELCDDYLNSAQINVLLNEILESQLKRVAFDLFEFAYLDTQITNEFLDTLQNLFIDLVTDFFNYLIDIACHANYKMQQLDKLLKVYGCLANQENYKYMQKEISQNIIDYGDINLVGQSFQVNPEKHSVIVRQSIKHFYYVMDECKQWFTTKQHKNFDELINNAQFNIIKPLPQNIQVFQDQDDIPLMELCQEIKVIKSVKVQKNDLKGLKIIVKDEKTNKVQQFILSLSYHYLMFHKFEQGWEQFEDEKEQNILLAKFLNEQFDYLHCGHPEFKKRVASEKSIDRTTDDISGFKNAVIKFKKISAPKKPIRYGTSNRVIGGKALGMGIGVLIVDCLDEDIPWDDKLKNAGFTAISFGVLGILASRLPIVGIIIQQVIFALAVKSILANKVINDSQTAKNLGHLGLIITIGVGMTIAGSIMFPVAFWGAFFGGLLGGVGMGLYQKFLIPYYMDNIVEMMNKASKLIRINGVVRYKKYALQKLQIDEKFLETHKPSKLEDDQYFTLLMYCLSNEIRLISNASYSKQLEKLNQKNAKEEEKANLLQRQCQIEVSLKIWNDTYDYLKSNNISLVDNAKTCAEFVDGMLTNYKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XZR7</accession>
    <name>A0A8S1XZR7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1440067</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000144">
        <property type="protein sequence ID" value="CAD8206505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XZR7"/>
    <dbReference type="OrthoDB" id="296632at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000566">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000977">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23189">
        <property type="entry name" value="RNA RECOGNITION MOTIF-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13893">
        <property type="entry name" value="RRM_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="13"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="192"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8206505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="358" mass="41501" checksum="DF57A45C521975A2" modified="2022-10-12" version="1">MFNNCRDPPSQILLLILTQLPPSFPLTNEYLHKKFNEFGDIKKILIFERGKTNKAFVEFYNLKSAISARKQLNGLNIQGGKMIIHYSRLKNLNLEIVDNSRGTDYTQASSNSQNSDSILHSKTDDNIRVNLTNHISQSSSSRANSSPVRNEEINRLLDYDDDDFDIWKQEIQLNLTDMHPEIQTLLRQKQSKLLRIASIDSKVTAKMIYNVFTKFGNIEEILYQKQLQKAYVKFQTINQAIIAKEYLNNTQFFDSILRIYFEPLQPLQPTSLQDEYMIYYNENCTQKIMPLSSNLIITGVQDPTEISELIKMFAKIKDIKIHVNSLQLSMCSIADTLKIMAVFSDYEFKNQKLNIILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y0B3</accession>
    <name>A0A8S1Y0B3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1470022</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000147">
        <property type="protein sequence ID" value="CAD8207329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y0B3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8207329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="138" mass="16468" checksum="8A2D69F6689D161C" modified="2022-10-12" version="1">MISSIPITLDQIYGVNFKRVPILYNEMEGTNPLPINEIDEDTYSEKNSRQQHKHQLPPLQKVDIRAIGENQKKKQNLYRSPVISSQFDQHIGFRNSFYRQNKVNLESRQRMEEIKNKEQVLYTKYAQRFNVPKSQIWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y0X8</accession>
    <name>A0A8S1Y0X8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1470049</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000147">
        <property type="protein sequence ID" value="CAD8207376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y0X8"/>
    <dbReference type="OrthoDB" id="260519at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016717">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042759">
        <property type="term" value="P:long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006636">
        <property type="term" value="P:unsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03506">
        <property type="entry name" value="Delta6-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012171">
        <property type="entry name" value="Fatty_acid_desaturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19353:SF19">
        <property type="entry name" value="DELTA(5) FATTY ACID DESATURASE C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19353">
        <property type="entry name" value="FATTY ACID DESATURASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="2">
        <location>
            <begin position="23"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid desaturase" evidence="3">
        <location>
            <begin position="240"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8207376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="342" mass="40755" checksum="DB75BD48144DFF6D" modified="2022-10-12" version="1">MSPLPEPNNNGIQTPENREVFRFIYKNKEYDCTEYVPKHPAGKSFFDKMKDEKQDITEYFRCLHSKKALKILKSQKVVRSDLKESEDSKRYSHIKKQVKDLYQPDWTIEILLLLGLSLGLYLGVTTDWYIAIPTIALTQIVAGWQGHSTNHNRNPLLYKLSIPYGIIHGFSADWWQFKHNNHHIFTNRIGKDDDIDHTYQMWQYGFLYLKWKFDSIIASYNKIDIIYVLLHQIIVFQQKIWIYLVAQYIAGFFSACILIGNHEREYKFYKKIDKPFIEHQIITSRNYDWTDWLSNLLMGGMQFQTEHHLFPQIPFYRLPYAAKIINRELNKFGYKIHVGKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y113</accession>
    <name>A0A8S1Y113_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1480069</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000148">
        <property type="protein sequence ID" value="CAD8207701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y113"/>
    <dbReference type="OrthoDB" id="297260at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8207701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="322" mass="37178" checksum="659DBEDFF2B94D3B" modified="2022-10-12" version="1">MAQHLQDCELLTAKPIIHNGQDLVIQGLGAQEQCDSSFSSQHLCSSQQSSHRNINLSQSSFKKISKSSSDLQNFTNKHAHICAHYNFLGRCPLEHCKKRHIFVSEDDGLSELISDNIDLIDNLTSSGNKLKLPQSTYGTQLSKYRKCKSNTKEFCEKLEHKFISQEQIHFGDFPIQNFWNWNAFALQSFSIKSQPDLGNNQQENKYFSQLIKPKPLVDYKQASEILENSSSALNISNEIKKNLMSKLKSDIDKMQQKIKTISMLKKWINNQQVDQNEYKNLYSSLSQIQQIHYTNFLRITNISISNQAKFNNQFQNIEYLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8B3</accession>
    <name>A0A8S1Y8B3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1600084</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000160">
        <property type="protein sequence ID" value="CAD8210726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y8B3"/>
    <dbReference type="OrthoDB" id="288785at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052983">
        <property type="entry name" value="MFS_Riboflavin_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43385">
        <property type="entry name" value="RIBOFLAVIN TRANSPORTER RIBJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43385:SF1">
        <property type="entry name" value="RIBOFLAVIN TRANSPORTER RIBJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8210726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="267" mass="30146" checksum="DB707094AF3A3E26" modified="2022-10-12" version="1">MWKGFLSIFGGFLTHLVTGAFYFWGNISMNVSSYYRFNGYPDIETKTVSAVFPAIYFGIAIGSQFGIHLARRFGHKLISVVNMLCYCASMYAATYSNFYFFVFFQGLLPGVFIGIEYLIPVDNALKFFPSKKGMVTGLILCGFGFTPLVFNPILQKLLNPDNILPVNGYYPEEVAENLMKSLRIVTTIYLVLGLIGAFLMQSINQSDDHDDETYKQKLIQNDAQVSSFGSVHFRYFVQLVLDSSLSPTIRSMVSSIYQMTSFFHQSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8G0</accession>
    <name>A0A8S1Y8G0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Vacuolar protein sorting-associated protein 8 central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1550094</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000155">
        <property type="protein sequence ID" value="CAD8209653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="289913at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045111">
        <property type="entry name" value="Vps41/Vps8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025941">
        <property type="entry name" value="Vps8_central_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616:SF8">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 8 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12816">
        <property type="entry name" value="TPR_Vps8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vacuolar protein sorting-associated protein 8 central" evidence="1">
        <location>
            <begin position="604"/>
            <end position="760"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8209653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1408" mass="166494" checksum="A05B353453D02062" modified="2022-10-12" version="1">MYYLNYEVLRSRYQQFTQFNILDQLEPIYQSLKLSLESRDRNTFFQDKVQQQPDCFQVNPIYGFTQQANELYGSYGQPTQLCFYMHRQKRYLMIGTQLSVIICIEIVNGRVESIQAILNAGERQGRYGSVLAIQCWEKYMLVGFERGDIQLYEVGRKWERLHHESKLHSGKVMQVLFIPSSTIEALSLDEHGVIYKHAFTVMTVMDKYIVSYSKDKIYKNGKIYHIKRSTQQPKQKQSSWWNNAKSIFKKEDEVTELTDPSVKHSSDTDGQLTKFALDIKLMPEAMTQQLNQNIQQVVIAAILYSNRVEILKFYKGSLKCEIVYTLKRNTFSIDIPQQDNTESGELSWGEGYFQKSYQNAKLLCIRWGDIFHLVKCMNIEDELEIVEGAVYKMEKEKGNIIKSSFLTNNIIYAFSDSSWVALIHTTQFQFAKQEDGIVILEPKTRMLSKEDVEPQVAEISVSYEGPSKPCQVQIYENGIVSLRKNNILIERLQTWNEYLDDLIEKNQWEKAMHQGMLIYQGQIKILSEIASSLSARSEQMSQMFQKIAFTHIMFALKPLDIPLSQQEKENKIKKTIEFLLRVDNMSYALVVLKDFFKDIQQMKLYFTCLDPFIKNKQIAAIPESFFIDYVNFYQNEKEMIQQLILQLDLHQQEPTLLIKVCMDYHLYKAMIYLCTKQGDFITGLMKLMDLWENKCQSEQKEHCHIEKEKLRQFRIKLGYNILAYVRMCVKGINIVGERVPHNIYFDMLRGLVSFIFNTENLKQFILIDIRLSLQLLIQFMQHHIYANIKSATLPGVKFDIIDHVPEILKLMLSAIENTRQEIKTQEHYQIIYNDKTQQYHQISLTEQLNHFLLFYSQLLMQFPTQFTKQFKENLFLELLSSSYFQTLKQYNFNTQETDPIYLESYEVNKNAYLIHIYDTSQLNDLTNIFNESVHYPEFHAFLIYRQKDYLTCISNYFLMQNTIMKRNVFEVIERLLIHSDEKFLLELQEYIIDNCDALMTISQQYTNRIFKEYFHDEKLQMKIIQKLEKTPQNQLHYLKQYIKNEKASDQIRLLYLELLCQRAPKEVLKDVQNGDFPLDDAMAICEKYKVYNAAAYLLQKNGAIQKALDMLTLLFINRLKDCHKSFARSKTISEADSEDIFEKLNYIIDLCSKIEDDNYWFQFLDSFFKQHKLEQLTDIKVPESLRKLHGDIVSEVFLAMSKCIELEKLLNKMNQHYGSIPLRVFLKTNKSIQEKFAFELPSYNIAMRQTDVTYKTLNKKLFQQMNQGVCVDRFCGECNDRITKCTKAVAFQCGHSYHQECYIELHGNNQKSFNSLKLKNQKVFQECFICLENNEKYIAHLIIQASLRKQKLDFDKHLHRQANVVPESKQVIVQYNKIEKREKAIKKLKQDDFNKMYIRESSLNWKNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YAD4</accession>
    <name>A0A8S1YAD4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="9">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T1600087</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PPP phosphatase family. PP-4 (PP-X) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="9"/>
    <dbReference type="EMBL" id="CAJJDO010000160">
        <property type="protein sequence ID" value="CAD8210729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YAD4"/>
    <dbReference type="OrthoDB" id="1930084at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07415">
        <property type="entry name" value="MPP_PP2A_PP4_PP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000010">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="10">
        <location>
            <begin position="110"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8210729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="306" mass="34941" checksum="8EFA4E94AE52FE18" modified="2022-10-12" version="1">MSQSDLDRQIAQLRNCENITEGEVKALCTKAREILVEESNVQRVDAPVTICGDIHGQFFDLMELFKVGGDCPDTNYLFLGDFVDRGFNSVETFLLLLALKVRYPDRITLIRGNHESRQITQVYGFYDECLRKYGSLNVWRYCTDIFDYLSLAAVIEEKIFCVHGGLSPSIKTIDDIRAIDRKQEVPHDGAMCDLMWSDPDEIEGWNLSPRGAGYLFGGDVVDDFNRKNNIELICRAHQLVMEGYRVMFNEQLVTVWSAPNYCYRCGNVASILELDENLAKSYKIFEAAPQENRGLPAKKPIPDYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YIY6</accession>
    <name>A0A8S1YIY6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1790018</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000179">
        <property type="protein sequence ID" value="CAD8213463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YIY6"/>
    <dbReference type="OrthoDB" id="687730at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051266">
        <property type="entry name" value="CLCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10579">
        <property type="entry name" value="CALCIUM-ACTIVATED CHLORIDE CHANNEL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10579:SF43">
        <property type="entry name" value="ZINC FINGER (C3HC4-TYPE RING FINGER) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="1">
        <location>
            <begin position="281"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8213463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="700" mass="81586" checksum="F0BF723965359C43" modified="2022-10-12" version="1">MKSKQLHMNQRNQQQFPIQQQQIQQQNLFYDEDKSLNVQLLRTLKQPPHSIRQNNIDINFHLNQLKKNPFQNSNQQKVNHFKIGHRQIKQSQQSASLVRRDMNNNNNNNTNQLIEQIKNQYYSPQQQNVQITPYKLIQQSHNNQMSQFSRNINNNYIQLDQFIPQNRDQSPQLDFNQLFSRTNQNLFRYSELRKRKQQLLEEIQIKTETIFDNDEYIAPQENQINKNLSNNDLSSKVSFEIKCLQKTCKLKNEESQQLPAMISIKTQEIDSIETERRVGVDLICLIDRSGSMVRQKINMVQQTLLILLNFLSDQDRLQLIIFNESAEQLTPLKCVTEQNKEYFKSIINSITAQGLTRISAATNLAFQQLNNRKYKNNVSSIFLLSDGHDSQAAYDVEKQINIVKDPFTLHTFGFGDRHDAKLMTSICNLKNGSFYFVQDITLLDEFFVDALGGLISVIGEDVKIQVLSNPDQPYQDIKISKTYGNMWKQNNESYEIHFPQLISGTRKDFVFEIQIPQFMNNINNQQVKIIDAKLTIKNPLNGEIIEKLANLSLTFYNPDQEMIQIDTDPDVQTQYFRVKGTEAIDDARKACEQNKFEDAQARINHILIQIQQNKIANSLCAGIIQDLDQAKQASMITSYKTFGQKQMYQMVTNNYLQGGINSIFTANGKQLQQKQPAHYQNRIQQIMMSKVQNQKSRQHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YL24</accession>
    <name>A0A8S1YL24_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1740049</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000174">
        <property type="protein sequence ID" value="CAD8213167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YL24"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035929798" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8213167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="351" mass="41071" checksum="FF2FF38639BBC6DE" modified="2022-10-12" version="1">MKFVIGFLILNVLVVPIQMYTSLFSDITEFQNNVFLYPRYVLTLLFYQIRKLVLNKQKFSDISIQAFERQNMMFRCNASQGRLMVCLILSKGDVILRDVPSSIFIDNKNNLIIKRCKTQKIYRRKLNIDHQNKYMIIQAQQIVIYTEIINSYNIIIKHLLSTSNDISRIAFINENVFYILKGNSYKLFIFESSQIALLQVLDKGRDFPNQSIREIPLNTDLILETNNLSKLLMRIFNQTKQKEQFQGTMHPIVLKNKQEVHAKEFHKMVKIRWVIHNEKNLWALYDISTDEKIMKSYETYTQDSSIAKIENIFLADSGINIGNSKITLGGQSCSPALYQKQNMDIPNRTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YN44</accession>
    <name>A0A8S1YN44_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Proteasome alpha-type subunits domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1710055</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the peptidase T1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000171">
        <property type="protein sequence ID" value="CAD8212842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YN44"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000007">
        <property type="entry name" value="Proteasome subunit alpha type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="8">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8212842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="249" mass="28331" checksum="E6010FCFE9A3B700" modified="2022-10-12" version="1">MSGSMYDLSVATYSQEGRIYQVEYATKAVENSETVIGVKCKDGVILGAEKQKFSRLLEDNTNKRIYNIDLQTGMALCGKLPDGRNILQRAKAESSQYQDMYAIPASAHVLSDRVAQYVHAYTMYSGVRPFGSATFIASYNTFDGYELYMIEPSGNKYAYHACTHGKGRAVCKSEFERRNFKDLTCQEALVYIAKMLILAHEEFKEKKYEYEMTWITDNNGRKHQNVPQDLINNAVQQAEQLIEQDQIGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YPH6</accession>
    <name>A0A8S1YPH6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1750037</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000175">
        <property type="protein sequence ID" value="CAD8213214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YPH6"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="156" mass="18559" checksum="1AA164CEFC5E43A1" modified="2022-10-12" version="1">MESYKKPILKRQEKQEELNQQIAIMRKNNNLQLNEISEKLITEFSLPINKTGEICKFQQTSMKGLSQEELFSNISYLIQYDKENLGQDSKIESVKSKDSLFLIEIESRLGHLIYMINQTFKSQLIFYKIFKMIIKFKGLSYYHNNFKKTQKQIKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RV21</accession>
    <name>A0A8S1RV21_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0010346</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RV21"/>
    <dbReference type="OrthoDB" id="303555at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8131908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="241" mass="29148" checksum="48EF25A39BEE8B1D" modified="2022-10-12" version="1">MNSFIYSFKNPSSEFVESANFIQNKRPNQFCLKIFNQQSYEDFKLEDQQQQPLQQSQDQYPQLSISQYFLNPQLSLELKSKLPSLDKYIKKKKIHKKAKQQSAAIRDYKKNICRNILRHGIKGINNQQAQEFLLEKFRNNNKKLIEFQKFYQQNLEIISGFRVLKDHLIIQTDEPKEEQERKRIFQQYLIWFLSSQATKCILQSEANNIAEYIKYKNDVLLYYVQQPLQWYSNKPLWRRIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RV99</accession>
    <name>A0A8S1RV99_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Granulins domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0010391</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RV99"/>
    <dbReference type="OrthoDB" id="305117at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000118">
        <property type="entry name" value="Granulin"/>
    </dbReference>
    <dbReference type="SMART" id="SM00277">
        <property type="entry name" value="GRAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035733177" description="Granulins domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Granulins" evidence="2">
        <location>
            <begin position="55"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8131998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="96" mass="10443" checksum="B72917C53CD563DD" modified="2022-10-12" version="1">MNKTFILLLILSVLILTAKSLETNDKLEIQDTFTKSIIQQILRKSYLGTNCPPEQSKTYCSGSQPCCKVGNKYGCCPYSYGTCCGDGTCVPSGGRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RWX5</accession>
    <name>A0A8S1RWX5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2HC/C3H-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0010625</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RWX5"/>
    <dbReference type="OrthoDB" id="297947at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026319">
        <property type="entry name" value="ZC2HC1A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049899">
        <property type="entry name" value="Znf_C2HC_C3H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13555">
        <property type="entry name" value="C2H2 ZINC FINGER CGI-62-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13555:SF5">
        <property type="entry name" value="ZINC-FINGER OF A C2HC-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13913">
        <property type="entry name" value="zf-C2HC_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52027">
        <property type="entry name" value="ZF_C2HC_C3H"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2HC/C3H-type" evidence="8">
        <location>
            <begin position="18"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="C2HC/C3H-type" evidence="8">
        <location>
            <begin position="121"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="C2HC/C3H-type" evidence="8">
        <location>
            <begin position="237"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="153"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="44"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS52027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8132466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="348" mass="40816" checksum="AF46DEC6548EA5FA" modified="2022-10-12" version="1">MEDQIDNEFLDENCENQDLQQCDICNRKFHSERIERHLIACQKAQQKQQERDKIIQKKKKQIEQKKQHLQQMDVEIVKTNWREEHQKFQEQIQYNRKLKQLEIEGQDVTQLKPLETKVNSNYVFCEYCERHFAKNVAERHIPKCKEIIAKPKPPRKKTVEMIQPSQPQLQEKRQAQVSTPSTSSQMERKPIIKKQLSDSSQQFRPTSLQKFIAEQSGKANLTNIGFIDCKARATAIQDTECPHCNRRFISRAAERHIPICEKLTRKHSFQIKYQSRKSVPIKQNNLMMINPTAVLPQIQRKGTKQLEAGNLPPIGNNQKFCTECGNRFQANHKYCGGCGRKREPEIVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S0T0</accession>
    <name>A0A8S1S0T0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sushi domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0020277</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="4405280at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002895">
        <property type="entry name" value="Paramecium_SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01508">
        <property type="entry name" value="Paramecium_SA"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00639">
        <property type="entry name" value="PSA"/>
        <property type="match status" value="25"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035814336" description="Sushi domain-containing protein" evidence="2">
        <location>
            <begin position="16"/>
            <end position="2548"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="3">
        <location>
            <begin position="140"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8133192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2548" mass="272150" checksum="E278839DB313B60F" modified="2022-10-12" version="1">MKIILTLCLIALVCSQQVFKSNECNDCGLFKSQNDCEGYKLVACEWIGASGTTPAKCQKKDGGGSQQGTFKPYCELVEKPEVNCSKTFGCAYLDSKCVHFAGCSAYVKTTPQECQAISYRCVSDGFACIEAKECKDYNESQCEITPSVSGILKCKFDNGTCRDFGCSEADLTFTTDQQCSDWQSTCITRGKGCWTKPLPNCSTYTGTEEQCNSFIGFDGNCELAKDTTNCKARECAKASNTISTDDECKSYQKGCITTGKGCYLGTTKPLCSTYDGDNTSCVGYIGSDGVCEGDAGGTKCRARKCENGNFKTDEQCKDYQKTCVTNGVKCVSALQTCSTYKGTATGCSAYIGSDGYCTGTSTTVEANCVPKVCDQAGDTFTTDEACEKYQKGCVTTGKGCSTKAALKKCTEYDGDSTQACNTRIGSEGRCTWRSGNKCSPRDCTSAPTSTNTNPLCSNWFTNCVTTGSGCVSATTCELTVKQQSCEGTKDCVWQPICTENSSCSQFKRKAICMANTARVFDGYADVQRTIEKWVTQKCGWGSNGCENLQCSHLTGPFYSIHANCQAEMSTCISNGVDACITKVECSKLKGTKDTCQAYPGFCTNTASATETTNCTQRQCSENVDAQDNPACEAWNQGCISTGKGCVIYTTTCNQLKGDRDTCNKLFGYSSGTITNYKTVQCYNDSTATVDSFCKIKTCIMATGMTEGSCDGFLSGCAFDGKDSCVDPETATCSDYYGVSSFCEGVKIKDQGDKYCYGTATAAKCTIRKCEDNADANTTDDICEGFKTGCVAKTTGGCIAKTAIACSSQTGTSETCPNFSGSTGKCQRYNTCQDRACADITNPNGHQDCITYKSTCRFLKTGSACINHNVCASYNVPDSTATSQAKFDYCTTITDSNGKLCGYKTGNTCADRSCDQMLGTYSTTLVCTSYISTTTTTGTGTCKLAGTGSTAVCYAPLDTCAYTHNISGTDDAKVTECQKYVNDKDIKCTYITGPQCTVQSTCEKIITQTDEKTCNDFLYPNGKGVCQKVTDSTCLTTVEVTAGCTSYKIDQTKTEAQKKALCQSLYVIDDDNNHGAGTGVFTKCIYKSTAPSTCSAISACADIPSASSQADCTNQKAGCLYFNGLCYAQLSACADATIPSSATTDTLKSLFCHSMKTSTAYCKLNNAKDDCEDNADTDCTAFDKSTVTGGQWDSATITLANFCAAQADNTKTKFCKAGSDPKCAVATCEDIQNANSQGDCDNRILGCAFSNGKCRTNVASESACTDAVVPADISLASGKTTFCQSITKSSKPCTYNEHKTGTADTKCAATGACSTYNVLPDDAATKLTYCNSKVDDKFHKCTFTTGASNCRDYDCFDISNPTSQISCDLGAPGKTCTYISGTCYNTTDGCAKIPTSGTDKKAYCELLTSATIKCTYISGDFCTPRLAACKDYDVTNATSKSTTCNSLKDTTDKECTYIWGDKCVKLDVCTSYDGSGSNAGENLLGPESGSEETQCKSLKDADRKYPCIKHDTDAKKCRAQVCADNDNDETGCASNVNGCIYYQTKCIQKTTCALYTVPGDLAGNTEKQAWCEGVTDSNNNKCKWDGTKCADRTCNDAATANFYTTSDCKSYLKTCKTNGQKCVDETSGCSTFTGNQPYCEYLLDNTAKDYCKLNSGAVVSGACQIRTCYDNVSAQSDSDCETWMKGCVTRGKGCIPRNKPCSDYRGTKQSCEAFKQFSHVDSETKLDVYILCSGDATNAETSKCKDRKCSDNTSATSNDECQAYLNGCVTKGTGCVEKTADCSAYKGTRDVCSKFMGSNGVDYCYNVSNATETSSCQKKKCSDITGTDNKTCSDGMKPFKVTDKPFCVYNGTGCDTYGKKCEEFKGTDNKDDLNSCSKYIASNGPCKATTVGTIVGSCAKRVCTEAPNTLKTDKECSDYHPDCQTTGYGCTSTIKCNTLTNQDSCKSRPQCTWANLCEPKKNSCSAQSGTSQSNCVNTVITYEIEKVSYKKYCAWTETGNVCRDQKCEDLSATINTHKQCNDFDKGCTTTGSGCITMTVCSGYKTESICKNASYVRDTKTGELGRCGWENNKCRERICQDLSGKTDAECDAHLSGCKTNGTECVQALSCSDFKTQQYCLSSKSGPCLWVQSNSTCYDYDRCEDALVKTHEGCQAFSPLCTTNGETCIPITSCAKTVLKASCVKGTDGVCGYLPTDKCQLFDKCSDAVSNDQNACLAYGGCITDGIACVAKGKCGDYKTEISCKNNLGSDGICFWNGSSCKLKECSDLIGTTYEGCRSQVISSGTCTTDGSKCVPLGLCSSYTEAGCYQGTDGTCIYSYPVGQTTGAKSCRLKQCEDIDKGINNSACSNVIPGKECVSNTKNCIPKAACSTYKTLEACNGGGLDGTNKVVCAFTPNSATDKVNGTCKTFTQCSDANADPTACETNKSCKKTQSGTTTSCVPHTCDTFATSTDCQPVPSFDGKGFTICTLQNNKCAPGDPGTITDPKICYTKSAYTYTWNQTTNKCEKCLAGSTQPNNTNNNTDNTTTDPNTDDYGMILSIITLGLFGIMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SAG5</accession>
    <name>A0A8S1SAG5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">U3 small nucleolar RNA-associated protein 20 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0050476</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8137048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="298515at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052575">
        <property type="entry name" value="SSU_processome_comp_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046523">
        <property type="entry name" value="UTP20_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17695">
        <property type="entry name" value="SMALL SUBUNIT PROCESSOME COMPONENT 20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17695:SF11">
        <property type="entry name" value="SMALL SUBUNIT PROCESSOME COMPONENT 20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20416">
        <property type="entry name" value="UTP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 20" evidence="3">
        <location>
            <begin position="1688"/>
            <end position="1890"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2544"/>
            <end position="2574"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="477"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="2549"/>
            <end position="2574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8137048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2574" mass="304044" checksum="BB3ABC909DEAEFA1" modified="2022-10-12" version="1">MVKYIPTKRQSKKETLQEPKQAARVIRHYHLEEKVDKRHRFKTTKQQREEIDINLQHRIEDIHIETKSDISGCYFRDIIELKRSALSTLMEKKYMNESYNYAQSLPQILVNLKQLLDIAYTYLKEKSSKNLVLDTLPYLIKDTREEFYPYLERTIKEILSQIDIEQVEHLEKSFLFIAYAIKYLEKVLIDKFEPFIQLMQDECFSSKFNIIKKLASEAVGYLFRKNRTLIHRALNIVNHQQLSYSIYISIQSIKGYLAQNTFKVIDEIWKTENNELITEFYIVLIQNVVEKPHQSPKIMISLLEIFKDLKQRTQQLDQTILIELFKNNKKKVTPQIVFEVITFVNNSDSMGYLLRLRNIQVEEIIISNNSFVNKILNQITIEEFSSIVDIILQQPTHQSSNIYKLLIAATFQQLFKQKEEALILLLKLDEIHCIDKLTPDQLDQLYNSELFNKLLQTDNNLYILSLCKLIQMVEFPSQIKQQILNKLQETLNTLNNKTISQDQSIDYTETIEKIEKLSQLKLEITEEVELLYAEQHQDNKSFEDGIIIKILQNPEIQQIELNRTIFNLFSRINLQTKEFFTLINNIPQNLLEIGLMSRKRQIRLICAQKLFQLENSEQLQLMIDIDSEKINIDNERRIKNSIINLKNLVNKSTNQKQIERYAFGLAFTKFLLVRSTIRELLSTVLSKCNIQSILRIFLITHQEYQKVDISYLEKHCYLEQDEEQLESQVCFDEVFAYAIQHCDLNQNIKQTIFDISTKYLQQQYEEDINCTKVEQYYKRRYGDKTSQFHLRMLYQSSQIELFKHWLLKDDEQTQMIALNNIKFVEIQNNLQEYHGIINEFFSSMEQMINGNTFKGEIIRFQLDLPQHLQEQYKLYILNVLYPKIFSKQKLHKKKHKNSAIQVRRNAVFEQLSNLNLQNELITLIQANHGFHNDLNNVPIQIKIQYLKILKNLIEKFGYQINVEQLEKQITIIRQLLNQAYELKQNENVTSLSKELWKVGIKRLCQIVNKYQSEQTYTQMIQDNLQFCLQDNGFIQGFEKIVIDSKSQPSGVLKLLQSICLIKDYQQFICQNHELLLSRFFLLYDFTYIKQIYLNQDEDGRLAHQLKQQISQKISAQRNCIQILNSILYSIYQLSEPSATIEIEPNQLSSLFSEQYGELLLSCLINNVRSNIESDSEQIKDLNMIYLTYTILMNRKEINNQLLNQCLFLFSKQINNPKLYDSQIYNHNLMGKLLNNCIKCILDKQIVVEYELVVDLFQSYSQLLILVKNLTTRNLLVDVIISMAYKFKEQYPLNENDVYLLKAMNQRNQVTEEIEFEKCLEACLQFQKIIHNHQQTTELILQNCFYLLTSSELSIRISVLEIFKSYLSKNIQSVYIKQNYLPQMIQLMKVNEETSVKAAIQLFEQYLLCIQIDEYTQQILNNQPLNMIDEQYDSNFCEFKVLFENENADQNVFNLLFDIQLTKRIKGVQLIQKRLLQLPILLIESVSKIMLPLLDFYIFYAGGELNNHKHKLTDANKSHYLTLQDVSIQMYGYICSHFDFDRRISLMQQLTKKLEKSMIGNKQQLSNAIIKTITVIASNKDDNFNVIKLIKERLQMNSESKLQDYLQQYISIEEEQQNTQIIDFEFSYRSLIQQEKELAKVREIKDVSNQFWNKIIDITVDEIQEWQERSNILLKQIYLPLKNLFLLDEKHRVQLSISLTELIIQLPPENFMIELNRLVGKLCRLLKSKDNDVRDETRKCLLDLSRKIGPIFLKFIIIQMKSILIQGFENHVLSYTLGLLLEQITEIGQIDNCLEEIMEILLDEVFGRLCTEKDTETLPKKTKELKSKKGMQNFKIVGQLINFVNSLPKLIGTIDYYITQYQISHNLQSKLRLIFEHLYLGLINNSSVQNLVQVIYLMICETSYKLADSLKQTHNLDFTYAQGTYKRITKEDIMSIQLPSYGSQSISTYIKQNKLKTSSTIIYGLVNFISIKLLHHNIQNVEDSPLLQKMFSIILDCLNHKDMITECVSVISAMIKKFDWVIQFKKQIVQNAMQCLATITSTDEQFIRDSFLLLKAILDSKLNEFTLQQQKTLVHFIDLYLESNEALECLKLIIHEIWPLPQIYDIIMKVRESMLQTTKKTQHLCQQIYINFLIKYPLSSQVLQQHYDFIIANLQYKYFGGRQIILQCLQQIFQFFPQDTVNKLSEISYFSMIVALVKEDVDTLKEQLINVLNILIQKTTQDKIMKFTQNSLIWMTNDKLKVQLSGLESLYILKDHLKYYQSKFILSSNELIDKCITNWNEWKNTIQAKNIQNKNLKNSQWANELEAITVSEYPELQESLHSLLKLLNAIDENQLILIYEQNTICKLFKCVSYPHQETANQAIQLFIRSKHLSIIHTQIQIQQDHIIKNMQNITKYLFLIKFARNQIIINQELTFQFILDYLNLLKQLDQQSIIQLIFDCLLKVTTNLMAKQLKFNQGIENLMEFYQYIMNNGYESNQKMIESILKIQSLQQDLPIIDQILKLIEKQLGPQEFISKYSAIQQELNKVKGEKKIQTKMLAIKDPQKFQEKRQRKMKTKVMQKKNKLLKKRGKIAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SDM8</accession>
    <name>A0A8S1SDM8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0060438</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the inner dynein arm light chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8138076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SDM8"/>
    <dbReference type="OrthoDB" id="273640at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019347">
        <property type="entry name" value="Axonemal_dynein_light_chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13183:SF0">
        <property type="entry name" value="AXONEMAL DYNEIN LIGHT INTERMEDIATE POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13183">
        <property type="entry name" value="AXONEMAL INNER ARM DYNEIN LIGHT CHAIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10211">
        <property type="entry name" value="Ax_dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0243">Dynein</keyword>
    <keyword evidence="3" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="150"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8138076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="236" mass="27395" checksum="F0018AB64ADC9AE7" modified="2022-10-12" version="1">MNDILPAPSLVKYSNPILISNSQKKQVKQSPPSNTEDILNAILLPREFNHQKNQLQIQCVSPAPSTKQDVLELQDKLDKWLSQRLARETGLCPIREELYSQCFDELIRQITINCAERGMLLVTVRNEVQMVIQTYQTLYASSIAFGMRKFLSEEEKKAEYRLRIKQLEQDCSELQKQVENLEQRLLETKQQDQLKREQTKETHLEIISQLKTDIKSKVSGDLEKILTGQKKLPDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SEM5</accession>
    <name>A0A8S1SEM5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0070226</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SEM5"/>
    <dbReference type="OrthoDB" id="297478at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="368"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="241"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8138643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="394" mass="46048" checksum="54E63EB9BA834497" modified="2022-10-12" version="1">MLFKIDYSTIEQSKRILITNIAQFPILVRILDHDAYNFIPTFSLLKRDDCKYIQILKKQYLNDYRDIIIDAIEFDENKIDSFSVPPFWNEKVQGCLQTQSLSLPAASNVNKSNSQISQERLPSINQIFQDQKVVQINNDNTIKFNTQILKQNASNNESDQLINNQQRLNSLQSQQNNNSIKIEEDDKNQQITNHHILRNSTNSFESYHQIQTKTIQQPIITIPNDFNQFLDQIDCNTSPFQHLTPSQQQSQTHSQKNISDNAQQDLSLSDTTSIQRFEQQKQSYLQQSKSQFNEKPKLRVSQTLITHPKSVIESQKYQDQNKQIESQIYKQIAELKISKEALSTELQQLKFKALFHTKNKQSTYQYHIYIWHLLLTSVVFLIIGSVLKKMISLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SEQ2</accession>
    <name>A0A8S1SEQ2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0060267</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SEQ2"/>
    <dbReference type="OrthoDB" id="298371at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8137749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="406" mass="47999" checksum="86A41F683F0E6532" modified="2022-10-12" version="1">MQQNPLQLLLSLLAAREEVIFMIRVIGHQMHEDCRNKLGTLREIQDEIIRGEQKRENLEHYLFQNLNEGYRKLFLPKIIQKYTKITQYYLVKTQIYFPNQGQVICQEVINQPNLRNSISHILQESLITILKFICNLQKQKLKHELAQMFNYEQEDRCNRNEKFYSEPLILGVIGLRYLNELHKKYCYSVCKHNNRNVYDVLFLDDNTKQLYLYGGGFSSLISSITKLKDKSVDNTQSVYIPIESTLEIITQRYEKLLKEIMDQNYHVQKSVNKYYDQYKKYKMKPLLIKGYVTPKKQGQDFKSQRIQKRSIESGPMLSDMGRYSEPISRNPLTQDSLNSVRSKQAKCRTQKQSDIILSGFISPKQQTIVRKHRSLKNYDPSLLKPTILQNFYDSNIRINQNTKKPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SFG3</accession>
    <name>A0A8S1SFG3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0070205</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SFG3"/>
    <dbReference type="OrthoDB" id="293289at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8138618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="100" mass="11746" checksum="AB8382EC3452EE12" modified="2022-10-12" version="1">MHNFLHFLIVQIFKIYSLKFINLQLDIQQNTKKMTQNFNNQTQITLMTERYNEQYENNKETEIKMQQGLKKDLTDCVTKVVGEVAMKVCFALTVAFCIGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SG66</accession>
    <name>A0A8S1SG66_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0080011</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SG66"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000454">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000574">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="156"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="11">
        <location>
            <begin position="415"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8139263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="488" mass="56604" checksum="D137304D111C3C26" modified="2022-10-12" version="1">MKNSICQRNLVQNFIIDGGAYRANVNQSILTSRQSRLPSFKNPPPISTPSSPKEQKMMWKTNIGFQNDLSFISNRNKSPDQSSQKKSYSPQLYSITRKPFQLQLGGEQQMLNHHSKSREKRPMRNSIYSRPTSRLIEESQTKCMPSTDSEINKNSFKFHYILGKGGFGKVWRVELMKSRKLYAMKEMSKAKIMAKKSVNSVMNERILLSQLKHPFIANIHYAFQDREHLYLIMDLLSGGDLRFHIGKMKRFSEQHTKFVIASILLALDYLHQNGIIHRDIKPENIVLDKNGYPRLTDFGIARIIKQDNAQETSGTPGYMAPEVMFRLNHGIGVDHFALGVMMYEFMFGRRPYLGKTRKDIRDAIIAKQIQLKKGDLPLGWSLEAADLINQLLSRKPQNRLGFNGTQDIYGHQWFAGFPWKSLHEKQLASPFKLSKCSEENYIREISSEHDSLEKEIQENSILLRQETIQNQFQNYSYIKEQQVTIKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SNP4</accession>
    <name>A0A8S1SNP4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0090411</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8141046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SNP4"/>
    <dbReference type="OrthoDB" id="296869at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398:SF0">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="326"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8141046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="799" mass="95015" checksum="147E4EDFE1E9BBC8" modified="2022-10-12" version="1">MKLTTILRRLDIFGVPITLIGFNRLKYQTKLGGLVSLALVGWLTYNIILQFVSLISKNKLLVTTNSIFSDNPSLIELNSQNFMFALQIRQSDYVDKPYFTFKLEHLSVTNGFNKTTQIIPFTKCTIKDFENLYFYNVNMFEQLVFNNYTDWVCPQFGNKTYALQGKFGANLFQYLKISAIPCKPGQYDYDYQCPSYEETIQYINSIDGELNINLMITNVNVNTAQTNSYTGFLDRDYYMLLNPLKQFGSVDMFLRNWDVYTNPSIIDDGTDFDYQNYVVLEKQETSEKQNYHEYDIAKDQEIAKLFFRTSQYSLYTKRNYVSIGDFLAYVGGIFGSLYTLGSLFMSLYAYEQLWLKLFKVHYNLIAYDRNCKSLNEIIKNKFSQENILIPNMKSFFLFFTKQGIQNIKTYIQSQTIIMEDLSVVNYLKTVKDLQKIKKILFNEKQTQILDLYNKETIAITKYQAQHSHKLEKGKKFLYGQHFQDHTLQTLRKIIDQYQNLLYDKDKFQLKITQRLINIFDFDFQEVINNWENGYYQLKTLNTTNYDNVQNNRMPQLSQQFAISKDITKIINFRNQYTLSNTEIESLLKTLFFKKQTLYLDENFNIQYEVQINNEEEKINYVLTIHNKFQQYELDIKQSDILIQHQYTARKHKIECNLMKNKAISLLDLINCSLEVTINSNYTIYLNCSLNSNIQYQLKVINIPKDFRTHYYIYLDDHNKERFQLLFALMHQQKFMMSNFENFECMFQFDSNGQSAQYIEIFMNWPNNEIILNEQIISNKPYIQFLQVLYDIIILYLQNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SNP9</accession>
    <name>A0A8S1SNP9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0100182</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SNP9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8141540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="99" mass="11452" checksum="62C024D18928E0E0" modified="2022-10-12" version="1">MNDTIDNELCLQEMKEIEKKIIKYLCEQLKLEQLTDPQLKQLNYEDAKKKQVFSFRSLDSDTETSDNISSTPSNRCSLQIARSPLILKRLSASISEQLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SPT3</accession>
    <name>A0A8S1SPT3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0090061</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="293389at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="218"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="287"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="317"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1122"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1320"/>
            <end position="1342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1443"/>
            <end position="1462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1649"/>
            <end position="1670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1407"/>
            <end position="1427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8140412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1712" mass="200823" checksum="5EC6D8D8C4DDEA96" modified="2022-10-12" version="1">MENFKHSLQSKLENRLQFIIGFTQFCLMKENYFLWILAMIVSVLQKVAIIKLAPLLNNFKGVTGSQDLELLFKMCVPTMYLPENLGYDYLIACQSVFIVLFSIPAILVGVSMSDEITFFKQTKFKNKRQLNISQSDNLSDCIQIMHQIYNQNSYIEGFQHRLMSDQSNTSLQIIRLRQIAIFLNYFPHVFLIPILYFLLQINLILEKADQTQQVVGQILNVILAFQTLFISSLSLVWCQVSFTLHESNFMRLRFSYLQWLHLGFSIIPILISYSYVRAGNVIYEDDFIHIVNLVFVILAFLIDALDAYITLPYVQKFKYSTNVFMRAIVICLTLFFICRLSDQQIICLTFIVSPCLSRLFIVLQDSRIGNVLTSTLKKEKSIQIPVIQQYQQPQQTLNTHESEQGLRIESENIKYKDFQSNIIKQYDMSYFIHFIRAVQLMKLTEIGVLTRNSGVQLKNERSKILMVCIILLKTHTENCNNQYCFCRGLKGEKRKIIEKYEIVKEMRMYDDFKILNIQFYIELIMRYLRDYSKLVFQTEIRKTHPNIIYLIQILVFLCNSDECYQAQLQILDLKHLLEKGSSLQQRLAFELLKSYSKYQILHRFKEDITRQSIELTLKYEDYRYTELERDRLLKLVFTNLQYKKQFLIKLNDETHSYESLEELYLQLNQQNCKIQKELEYFNLYSPGRTSLYCYMLYCLEVTNNYDQFIKLCNLINTRESKFFEYPSFYYKTEEQSYKVGYMSLNLTQSHMKRGEIISFSKNFPSFVGYTQEEFRQQVKSLDQLCYPSLIKAHDEMMEMFVLTNRPMVFRQPLPFLTQQQTPGILSYLEIFIDISFNFSSNVLPSFCFMKEIKQGIRQDGLSGFILLDSKQNIEGITNNALECFEMSNPSQLLGKSINQFIPNFNSLDAQLQHYLEEYQKENCISGTLTTANKLVQHFTVSQDSIEMILDKETKFQLDLEVQLSYSLVNRMVFKVYVLKIRSPYKLGAVCSDESPLSKSLDEYSSEFEGIQLKDYIMPGVGDNEIPQFTTQPDPITLRSQMVAVEQENQPLKQQEFLCSDHHDMISKQDSSEKDLESPEQPHKQIYINSISSNSSKQSEIQRILVKSHFYRKFSEMNTKPKLLYALLIINIFSLIAILGFATANTILYANWQSNIRSNIYILQAFTMTAYSRATMQGTMTLIQYIQMDQNLSEYGMYYDYNATLLGQLQTVLDRNIEIFSTKMDEYSENSIIREVFSTIELEQLDIFENVDKTVKGWTALFQLIYMIRSLHISDFTDNFQFFKSILYLLRSYSKYKDAYSLLNIRFTDEINSILASQDNIIDYVTFTFIAFYVVYFCIAFLLQSSYIQLCKKYIRCSEQISHSAIASELIHVNQLLLINQDHFDLYEYHFSLRDKFHQDEVDKQNNNYLSGSSSANNNNNNNNNNNNKKFIKVQEQWNLKRQLTFSFHLISFTISLITFLLIRMSDKLQIYELESKLTIYQKCITFTESLSEACLTNFILQSNKYLVKNEFIDPEDPAQFFIYTNESYTIANSILGEILYSEGTVSEELKVLFSSNVCLVVNEPEICVTVNNGELQQGLLLPSQDIFQILRENIDLYQIQGSATQMLQLRHMLSESFMIDAISNFVDTVRQTVEQGILDSNNVKLTISIIFYLFIISINIAQICLMHQYITQKLWGIRQFVYLLPPKALYNEDSFYKTLTYLLKIDNQILYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SQ57</accession>
    <name>A0A8S1SQ57_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Glycylpeptide N-tetradecanoyltransferase</fullName>
            <ecNumber evidence="2 8">2.3.1.97</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0100398</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Adds a myristoyl group to the N-terminal glycine residue of certain cellular proteins. Myristoylates adenylate kinase AK2. During the asexual blood stage, may myristoylate proteins such as ARO, CDPK1 and GAP45. Probably by mediating protein myristoylation, plays a role in the assembly of the inner membrane complex during the early stages of schizogony and in the formation of rhoptries in the late stages and thus merozoite egress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 8">
            <text>N-terminal glycyl-[protein] + tetradecanoyl-CoA = N-tetradecanoylglycyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15521"/>
            <dbReference type="Rhea" id="RHEA-COMP:12666"/>
            <dbReference type="Rhea" id="RHEA-COMP:12667"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
            <dbReference type="ChEBI" id="CHEBI:64723"/>
            <dbReference type="ChEBI" id="CHEBI:133050"/>
            <dbReference type="EC" id="2.3.1.97"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterodimer composed of NMT and AK2; AK2 myristoylation stabilizes the complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the NMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.97" evidence="2 8"/>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SQ57"/>
    <dbReference type="OrthoDB" id="60315at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004379">
        <property type="term" value="F:glycylpeptide N-tetradecanoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.170:FF:000001">
        <property type="entry name" value="Glycylpeptide N-tetradecanoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000903">
        <property type="entry name" value="NMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022677">
        <property type="entry name" value="NMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022678">
        <property type="entry name" value="NMT_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022676">
        <property type="entry name" value="NMT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11377:SF5">
        <property type="entry name" value="GLYCYLPEPTIDE N-TETRADECANOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11377">
        <property type="entry name" value="N-MYRISTOYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01233">
        <property type="entry name" value="NMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02799">
        <property type="entry name" value="NMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015892">
        <property type="entry name" value="N-myristl_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00975">
        <property type="entry name" value="NMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycylpeptide N-tetradecanoyltransferase N-terminal" evidence="11">
        <location>
            <begin position="78"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Glycylpeptide N-tetradecanoyltransferase C-terminal" evidence="12">
        <location>
            <begin position="245"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="17"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00062182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8141968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="435" mass="51126" checksum="53212A4C10651342" modified="2022-10-12" version="1">MQQQEKQQESSDEEIEVQGQGEQQQQQQQQLTVVQKQTQYNQQLLDKLNKPHQFWETQPMPNINELDSLKPGPIQEGILAEVRQDPYNLISKFEWCNVDLRNDEQAQQVYTLLKENYVEDDDNMFRFDYSIDFLRWALLPPGQHPDWIVGVKVNQKLVGFITGIPVTLHIENQQTKVKMTEINFLCVHKKIRANRLAPVLIKEITRRVHIKNMWQAVYTAGIVVPTPISQTRYYHRSLNAKKLIEVGFSSLSARQTISRQQKLYKLPEEPKTQGLRPMKKKDVAQVTKLLNEYLKQFKLYFKYTEDEVKHWFIPRKDVISTYVVEKEQGVVTDFLSFYNLPSQVIKNPKHTHLRAAYSYYNVATKTPIVQLMYDALILAKNEGYDVFNALDIMENEKFLKELMFCPGDGQLNYYLYNWKLESNMLKPEEIGIVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T2S1</accession>
    <name>A0A8S1T2S1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">33 kDa inner dynein arm light chain, axonemal</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0070035</name>
        <name evidence="7" type="ORF">PPENT_87.1.T0150037</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the inner dynein arm light chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8145836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T2S1"/>
    <dbReference type="OrthoDB" id="273640at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019347">
        <property type="entry name" value="Axonemal_dynein_light_chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13183:SF0">
        <property type="entry name" value="AXONEMAL DYNEIN LIGHT INTERMEDIATE POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13183">
        <property type="entry name" value="AXONEMAL INNER ARM DYNEIN LIGHT CHAIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10211">
        <property type="entry name" value="Ax_dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0243">Dynein</keyword>
    <keyword evidence="3" id="KW-0505">Motor protein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8138278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8145836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="245" mass="28342" checksum="212ECBB8BAB5BDF9" modified="2022-10-12" version="1">MSDKPNIPAPNSLVKYASATLVSTSGKPIKDKKKGRDAAPQSITNAQTEDILNSILPPREYTMEKQQLWIQCVSSTPAKREDVILLQENLDKKLQQRQARETGICPIREELYAQCFDELIRQITINCAERGLLLVRVRDEIRQTIQAYQTLYESSIAFGMRKALQAEQRKTDYNNKIKQLETECQDLSKQVEKIESTIEDMQRQDQEQQEHEEAKHRDQVNFLKNANKVYKEELEKFLTGANVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T3V5</accession>
    <name>A0A8S1T3V5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Clathrin/coatomer adaptor adaptin-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0180062</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T3V5"/>
    <dbReference type="OrthoDB" id="10264595at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030123">
        <property type="term" value="C:AP-3 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017105">
        <property type="entry name" value="AP3_complex_dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22781:SF12">
        <property type="entry name" value="AP-3 COMPLEX SUBUNIT DELTA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22781">
        <property type="entry name" value="DELTA ADAPTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="8">
        <location>
            <begin position="35"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="652"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="652"/>
            <end position="665"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8148431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="713" mass="82353" checksum="B793EC792462E5B7" modified="2022-10-12" version="1">MFHLSQMDQIFLKSLADVVRTSRTTSQYDKLTFTTQTIADIKNEIQSRQESVRYQALLKLFFLAMEGNNIRWAEFQIINLMGSGDFQLKLGAQLAAHLVIDTQSQGLIMVTNVFQKEFKNGHVECSATLSCLGTIANKDLSDSLLPHVLKLTTNTKPLIRKKAIAVLSKIFTINPLNIPGNLEMVIQQLQKETNISVLACGISLFCSVMKVAPKLYPLFLSIVYDQISKQKSNWLLIKLVRISNKLISLEPRFQGKLIEHYTRLLNQTNSKSLQYELIYSIMKYFKNHSQLYESAGDILKQFLNHQDPNLRCLGLECLTHISSSAGLMEFQEQILESFKKSDYFSKLQILQLFKDFTNQQNFQTVIEFFIKYSDLESNHKIIESLIFIIMKDKFINVDDPEKLLLMYIPVIAQKIDTIDNAIRFKELLFELLTRVPGLKVYSDQLCNQILQTYAIQSDSLQGPTLNIVQRHTQLKYSLNHREIIFKSLIMIVGESVKLLKLEALTNIIKFLEQLNILNCYESSLSDIDSLLFKIYIQIIKTDPSNDLIPQIEQLIKNIYIKLHNISIVKISFFYESLILNKTKDQLDQLYNLYAEDLPPINLEAQKLIKPPEGLDITQSFQIDIEEIEKIKNSNNDQLEQKPIIQQLQQIESNQTEQEDQNTLIQKQEENEEELKPLNSKIDPIPKKVFTINRDEELPPGINQNDINKSKEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T9T1</accession>
    <name>A0A8S1T9T1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0180060</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T9T1"/>
    <dbReference type="OrthoDB" id="306779at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="154"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8148427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="258" mass="30111" checksum="B5B742EA5E72327F" modified="2022-10-12" version="1">MSTFISTKQESFIERQLNTQQSETVKQYTSYTTEPTLLKEDYAVKKIERPEAYQFWHKKEKYNSYASRVLNTNYRSCSVINPGQSFQINNVGSGISHSQVDNNNEFQCCTKQQMVVSVVIVSREEIEQPWREECLYLQSVIKELEKKKEIKVVKEIDYARIKELEEENHHLQLELQQAQESTLITERVTQNNSEVEVWKRKFQEVNHDYNEAQEKLMNVEIELEALKKEKARAATATTTVTRSTVRTGTSSVRQAGQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TAS8</accession>
    <name>A0A8S1TAS8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0170116</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TAS8"/>
    <dbReference type="OrthoDB" id="166212at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011:SF28">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="114"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="269"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="504"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8147742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="681" mass="78783" checksum="1637E164E729C9B3" modified="2022-10-12" version="1">MTSFVQSTFAISAKPNFEFLQNQVESDSDNEEQKQKLQQMHEHQKEHKKFKKLIDIELVKEELKNRPNVEMKISSIQKKKISKIKSVRSLNDLIIPTSPILIDQLGNLKKIKEIRSEVPPELMGRIYSELRYEFVPALNPVYRQGDQNKRFYIILEGKVVVMKPKLKMVGSSKIEFDDNLQQKTQGKNDDDPFGLKILFPDYIILKILFQGDCFGEAAIKLDTARSSTVFTLEDTHLLYINETAYLEYLNPYLSIALDNKIQYFGQTPLFRNIDPEDYMGIVLESKLVTMKAGEIVYDEGEKTKYIYFIINGEIELLKQVKKKSIILSSYGEFQSFGEVEIMNKINRYTKAKVISPRLNCYRIRKRMFFDNLGSYGTYENMKKHSAIIYKHWQLICNAVQKSINPRDEVYEAAQDDNQNKPNFLAKYILNKKLIESQGQKLSQIKVFQNLTDSNLKDLKNFSNDSQNLLQRVYSNTLQQYQAKLLKKPQMVICDSDASCIRNLNQNKQPTNTSINHDNNSTTSPLNENSVDLKLSSNRSSVIIKRESQQKIILPSLHPSLHLVGPQPQSLNTVLESISKLPRVPKDNLVLSLMYQQAYKAENPAKKAKEIQQVIQASYRNVQNRFQPKQHHLTEIQPSPSSNEHRMKKNLQISGLLSMRSQKKQYLNFVHPKRIVGISQIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TCU6</accession>
    <name>A0A8S1TCU6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dynein regulatory complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPENT_87.1.T0200171</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the nexin-dynein regulatory complex (N-DRC), a key regulator of ciliary/flagellar motility which maintains the alignment and integrity of the distal axoneme and regulates microtubule sliding in motile axonemes. Plays a critical role in the assembly of N-DRC and also stabilizes the assembly of multiple inner dynein arms and radial spokes. Coassembles with DRC1 to form a central scaffold needed for assembly of the N-DRC and its attachment to the outer doublet microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
            <location evidence="8">Cytoskeleton</location>
            <location evidence="8">Flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DRC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TCU6"/>
    <dbReference type="OrthoDB" id="305196at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005858">
        <property type="term" value="C:axonemal dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070286">
        <property type="term" value="P:axonemal dynein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060285">
        <property type="term" value="P:cilium-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003352">
        <property type="term" value="P:regulation of cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039505">
        <property type="entry name" value="DRC1/2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039750">
        <property type="entry name" value="DRC1/DRC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029440">
        <property type="entry name" value="DRC1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21625:SF0">
        <property type="entry name" value="DYNEIN REGULATORY COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21625">
        <property type="entry name" value="NYD-SP28 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14772">
        <property type="entry name" value="NYD-SP28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14775">
        <property type="entry name" value="NYD-SP28_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0969">Cilium</keyword>
    <keyword evidence="4 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0282">Flagellum</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein regulatory complex protein 1/2 N-terminal" evidence="14">
        <location>
            <begin position="31"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein regulatory complex protein 1 C-terminal" evidence="15">
        <location>
            <begin position="393"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="188"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="290"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="412"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="188"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF14772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF14775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8150260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="505" mass="60241" checksum="E668FD7335C5768F" modified="2022-10-12" version="1">MALLGKTLKGRTTKAGGTEQKRNIKIRQITKSQQEFEESQKQAQFKKEQLRSKIREEQKIVNFNTKKLMAYWRRTMRAVKTEQLKLDIELYSQNNKRELDSKEAFIQMLDKNLDEAEDQYQIALRNHLIHLENFHLLQESRNRALLEEFERDIRILQEEFQIEFDDMTKTHKQQVKELEDMIKTVEEEEKRKAEQAKNQHQTNREETKNKDVEDTSQMKQQLEDKQTKFYNDLEQMHQKYQSDTAKKTEDHTKYYDANKDMSKKIERLVRSIASKKAKIDLTKYKILQHTKECNARNQALKKEKENIAKNYQDLKQKMNKFREEQQRLLKELVNNSRNSVLKLTEYKELGEKILKTAELCRRLETEREKVLPFYEDTVDLGQIPENLKNDFEAIEKEQYEEFAYLNNFYKRYNKVLLDILAIQKQKEALQNESNQLQSLLKQYLDGLSCNDDVLSNPNPLFVKNFNIDLGEKQVIGETQQTIIEGVFNVRSTQMQLQGQRVGPFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TDG4</accession>
    <name>A0A8S1TDG4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0180338</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Non-catalytic component of the proteasome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase T1B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TDG4"/>
    <dbReference type="OrthoDB" id="10248542at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03760">
        <property type="entry name" value="proteasome_beta_type_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000074">
        <property type="entry name" value="Proteasome subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016295">
        <property type="entry name" value="Proteasome_beta4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF6">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001213">
        <property type="entry name" value="Psome_endopept_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR001213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8148979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="236" mass="26763" checksum="93706DD6BA03316D" modified="2022-10-12" version="1">MIFNSEISGQQQHTTSPVVTGGSVIALVYNGGVIVGSDTLCSYGSMAAFKNVQKIVQISQNTIYASSGEYSDFQQVVKELAKIDRSALQYDDGVHPSPKDYGNFLARLSYKKRCKINPLYLQNVIAGFHNKERYLALVDIYGTYLESNFITTGFASYFCKAIISNYWNENCTLEQAKQVIRECFKVLFCRDCRAHDVIQLAYVDEQGTHIEEPERVETKWDFNGFKSRANEKLHTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TFC0</accession>
    <name>A0A8S1TFC0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0190222</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TFC0"/>
    <dbReference type="OrthoDB" id="306786at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8149662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="244" mass="29094" checksum="7DE30C058048A9ED" modified="2022-10-12" version="1">MITNNYLTSKLLKKSNIENRQVNDNLSKNDKENYYKQWEESLKRKELLLESKHQELKIYSKNLQIQSEGLLKEKLDLGEKIEKYNRLLEQIIDQQTSDKQLKKSSSLTPQAYINSNKNGINECKERVGNLLKNIRLTSKSHLKTQQGSYFSNRTEFEEILFSKRNNHSSSFTGIINLRNKEELISKQQQEQQRNSKENKERFSHQNTTQDELINHQKSLQQLEDNQKYFYSKVYEELNKLKKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TG62</accession>
    <name>A0A8S1TG62_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0220341</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8152225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TG62"/>
    <dbReference type="OrthoDB" id="300338at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8152225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="135" mass="16326" checksum="D71A204918F25956" modified="2022-10-12" version="1">MTEVEKQKFRNDSEFYEIVRTFHELVTEINKSFHKGKKQYHKKNNHHMKQVQIQKKTKQIQKKPINLEFGRNQYHIKIAYKIFYLNSTSENSLISMDQWNPLQQSMKIVKQPEDIDISKKYVQIVEQQLEKKEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TGX8</accession>
    <name>A0A8S1TGX8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0200192</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TGX8"/>
    <dbReference type="OrthoDB" id="305978at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8150302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="104" mass="12338" checksum="1E0676A90CDB42A2" modified="2022-10-12" version="1">MHKFDTIQGKLFYTVYMMHQQREIDTKQKGILKDLIIQKHPSMNKIKTDNTLITRRSLLDLADVEEDQKDEQKIEKNRPIIIRPILTNSKYKSNQSLLSSPQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TJR7</accession>
    <name>A0A8S1TJR7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0210236</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TJR7"/>
    <dbReference type="OrthoDB" id="290562at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8151229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="426" mass="51201" checksum="ED35EB25ECCAECCF" modified="2022-10-12" version="1">MQSYSLIKKLGTTLKKIMTLQQQNNVESDYLLWFKKEDKYIITELGIKIETFQFLRFSLNQSNDKNIDELKCFKDKYGQFKEIKAFKGQNEPIILQIKHNNFIIIHLREKYQYIYHISLDKENNPKLVFQGKHNIKAKILVDKYLELFYVVYQSEDQFLIQIYQINDDLQKVEKLKQSQYPLNNNNFINQIDYITLYNKEILLLFRQRTAVIFYQNLSTYQIVSLQKQKKLFYYTQFLYTDTVLLMIETENNELILFQNKQNAIHNLNINFQISLNKIQMLSLESIQKIFLLDLIQDLPNLQEFCFWDSNDYEVVKLLIIGSQQTEEATSAIIYCLKYKPVFVIGIIKYLEYYCFLIDQDFKQLINSVFELIPGYLINNLTYEIAHYPNYINYLKQYNIIYSKNLTDSQQIWCMLCNMTDNKCLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TKD5</accession>
    <name>A0A8S1TKD5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Apoptosis regulatory protein Siva</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0220327</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8152197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TKD5"/>
    <dbReference type="OrthoDB" id="300522at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022773">
        <property type="entry name" value="Siva"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05458">
        <property type="entry name" value="Siva"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8152197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="16440" checksum="AA180DB96D6172F5" modified="2022-10-12" version="1">MFRQSPYYYEVRQEVFKEINNDLAKELSLMKISDQRLDQTVPITKKSVSKQEFISYFYKKQKQNEQVMETEEENNLVCKNCDSKPLNANTICVECSRLACQRCIYQCYYCGQFCCLICCIKIYLHNGDFVSCLHCSQNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TMX3</accession>
    <name>A0A8S1TMX3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Kelch motif family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0240142</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TMX3"/>
    <dbReference type="OrthoDB" id="290815at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8153313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="532" mass="62756" checksum="1616949FB9A85066" modified="2022-10-12" version="1">MSNHNNSSFLETSQRSQKILDRFMSALSNQDQDQSYYDMKQILDELSIDQFERQKEQTEEIKIIFTPHKCQKIAEIEKELENLMIQITNMQVCDSILYQTTLQKIKQVRKVITQIVDKYFANLKNQYNFVANELVQIHLAPIKQQIFQLRKDIPNYEETQYNEVLERHEQIKDDLHQLRSLLPKEINLQITNQSLYDIKDSLYKLISLKFKDQFKIVPSTFSGFKQQKKNLLTLKSKTGHLKFQEQQNSIYTFRNNTIYHTHIDYIDNIKQQELILNDDNYRLNYCNLINTNQSLYFIGQFYQDINTKQFKISQMVTKYNKQENKIEMMSELPINSYGGQAIKQGQFIYYWGGYIVVDNVQIGNKKGFKYLINGNKWEQIESMKYPRIKGQCCLLDEKICITGGMMNQYQGNALIEIFSTTQQRFLDPIELVNSFSYVPQILGYSFPISNQEIFIMGGYNQFGNDGNAQLINLFLKTIQKNSNGIQRIQNLNCTQPIIFKQQIYLINSEDRKIEFIQINADTLQVKNIIPIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TUW8</accession>
    <name>A0A8S1TUW8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0300266</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000030">
        <property type="protein sequence ID" value="CAD8157631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="291689at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035793298" description="EGF-like domain-containing protein" evidence="5">
        <location>
            <begin position="18"/>
            <end position="2782"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2390"/>
            <end position="2412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2485"/>
            <end position="2504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2516"/>
            <end position="2535"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2542"/>
            <end position="2560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2580"/>
            <end position="2602"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2637"/>
            <end position="2656"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2662"/>
            <end position="2682"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2694"/>
            <end position="2715"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2727"/>
            <end position="2750"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="430"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="706"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="830"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="950"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="1011"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="1078"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="1138"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="1252"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="1371"/>
            <end position="1403"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="1496"/>
            <end position="1529"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="1559"/>
            <end position="1588"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="1615"/>
            <end position="1647"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="1661"/>
            <end position="1696"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="1730"/>
            <end position="1758"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="1789"/>
            <end position="1817"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="1847"/>
            <end position="1875"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="1902"/>
            <end position="1937"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="2024"/>
            <end position="2057"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8157631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2782" mass="313617" checksum="8DEBCD076F673AB0" modified="2022-10-12" version="1">MKSKIITYFVLITLAMSQMTLVREDQMNFSVSDSNWNYSTTCDWIQSTTIVSQIGSCATNSFQYSFVGSTTRRSMYTVGNLPPHFQVKIVMDAYFIDSGNGMSTIEYDCNPSTTTTKSFQYKDNGAGGNNIKNYQNVCNTGWGNFQIQTQILTFVHNDNDSLQFRVCATLNAGKTFGMRSLQIYVDRCHWSCLKCETSSAIDKCKVCFNNPTVTLATAGTCTQCPTNYLYIEYLNSSKGCFTECQYYRVPDSNNVCQFNENMLPFKTYLDTTTFTTASPWVFIPDPINFNIVNVERISQIPCQTTRNYVGPFYSNEGYKLQLSLPYNITYIRFRVTLLKFGTWVDYSTIRILLDTDEQASIYTISNAILNRNGQMLLTETNCTSPTVTYYRLEAKLRSSAISPVLIIQGNMDNIGAQSWGFRNVVLDIMKCQSSCSWCDYDLKCYACSAGFLYKNRCVPSCPPYSIESPGICTDFDDPIPNTKYLIKAFYDSTNTTDTDIPAIVGTSSSDSTDFSTTDTFSGTAGSKIYFSYYLGKRVLGGPLVWNKAVFTQNFVLNPHYKFRIRFTLVLGDDFASQGDFRYTVGGYPETTITYAQGTTTLNNVGALTSDKYIQVDRTENHFTNSFQIILKCNTHSSQAINQAFCLIYDYYILILECTQYCTACTGPTWLECTSQSGLPTGMLTPSSCVDSTYYLDNSVTPAVCRKCSPTYCLECTNQYICTKCATNFYLSNGICLCYSWTYLTTSNTCANCHDQCSSCYGPAKNECLSCKDSQHRFINNNVCQCKNNYYDDGINNKCQSVCGDMVVTDGEDCDDGNTTRFDGCNNCKYECQKECSICVNGGCSVCSTGYVLNSAMKWCIPLCGDNIITGSEQCDNTYNYPTSYCDNCQLQCQTQCLDCQQGLCYDCDTTLGYYVNLSNLRCETQCGDGIQAGVELCDDGNITPFDGCYNCQFQCDQFCNICLLSTCIKCQTGYQLYSKTNQCLPICGDKYVTLFESCDDGNLITTDGCNQCQFQCQDECTNCVLGQCYECNTPGFILNQKLLKCVPICGDGIITQFQEQCDDLNSVFEDGCYQCRYECQIECAVCATGMCYSCQTNYYLDSNNYCVPLCGNGIVTKNEQCDDNNTLADDGCDSCIFKCDINCDQCIFGVCKKCITGYNLNYNQKKCTSICGDGIITKDEQCDNIQTEEFDTEIECINCQLQCQQECEFCVLGQCQRYSETGICVSICGDAIVSQNEQCEDQNSQLFDGCQQCLQVCDEECIDCRFGICFSCNNGYQLINQKCISICGDGIVTKDEECDNGVDKFKMDGCINCKFECYEGCNQCVKGQCLECKNDKGWYMNQLDQQCNSECGDLIVTNLEMCDENSNFCDSCLLVCDENCIQCTLGKCSKCQNGYYLDQNICTSKCGDLIQSKNEQCDTDDLIPFDGCYQCEYSCQIQCITCFNGGCIECDELNGWYLENEQCKTICGDGIKTGDEECDIDINIDQGNISLNKCFDCKILCVEYCKVCDKGKCTQCIDGYELNDQNECILLCDNQQSHLKQCDDDNLDPFDGCFQCNYECEDLCQSCVKGVCLYCKTGYKINKNGQCESICGDGQITEVEFCDDANNIEFDGCYQCKYSCPQYCKTCEKGQCKVCEKGYHLEYLNCITDCTDLILQQSEPKCQILQCQNECEVCVNGQCYKCKQGWYWNKMKLNCESLCGDNQIIGEEQCDYTSKLGQINPKCNNYCKFECPDDCLQCEFGVCKKCQVGYFLRGNICYSQCGDLLINQNEQCEDNNLSPFDGCYQCSLDCQVSCQICIQGTCEKCQIGYELIENNCLSICGDGIVTQDEYCDDKFDSSLSCEQCKFSCDKNCQFCQYGRCIFCKKGFELKNNFCQPICGDGLISGYEQCDDENLLAEDGCFECRYSCQHQCLNCYNGYCLECDIENGWQLTPQGQCISVCGDLKVTGNEQCDDGNEINFDGCFNCSYICQVACTKCLSGSCYECNTPGWRLENFFCWEICGDGLQVGIEECDDGNDIPYDGCFECKSQCEEACVLCEAGKCLDCAFGWKLNAQNRCETYCGDGYVIPQYEDCDDGNLIPYDGCYECNYQCVQFCTNCQKGICYECNVPGWQILEFQCIPICGDGVINGNEQCDDGNLIEADGCNNKCEFQCHAACLLCEKGICLECDAYQGFSEYLNQCASKCGDGLWEPLTEQCDDANNIDFDGCSKDCKIEIDWFCQNLQLLVSNCFYEKQPTIQLDLIDQQDNISTIQISFSTKMMLSTNNESSKIFLENQIEDEIANTKNINVNLIDLKIEDLSENYYSYTINSITPINYEPTLVIYLAQIELKTTIPSDKINVIFIVNNKIIVSENKTKLIKNVESIQIPTQIYISQSTEKVIKTFSEFQTFQSYSFLTISLISVFTSGYSNFYMACDTLQYLYYSRYINLPFPDNLQQYLNSLKESQLSNIATKKLGISVMNFDDNQNTNTENDQMPQSFSKDYLTYDFLNNASTSLTIFSISFAVYYSSLIVSKILHHITPSTLNSLGSIVGGSILTVRQKCTKFVNNFAFSGIIRVLTINFYELAFSSLLQLSHINFNDTKSVINNIGAIVTLLSQFAFIGVLFHKIQQIQTKKTTDFKSSIKTLFQQQEGLQHQKMWVMQYNTVLLVKKMFYIYVIVGMQQSGLYQTIAVALQASIFCSYLLVQQPFSKIDDLRKALVTESGMFLNSLSFILYSVYQQFQFNQETLFYLGWINIGTYTIIVSSNTLIDCFAQFKIVYSKIKKAFNNFIQSQLPSQSRIQPIFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TVB7</accession>
    <name>A0A8S1TVB7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CFA20 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0260195</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8154876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TVB7"/>
    <dbReference type="OrthoDB" id="10261083at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040441">
        <property type="entry name" value="CFA20/CFAP20DC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007714">
        <property type="entry name" value="CFA20_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12458">
        <property type="entry name" value="ORF PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05018">
        <property type="entry name" value="CFA20_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CFA20" evidence="3">
        <location>
            <begin position="98"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="338"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="384"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="350"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8154876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="497" mass="58373" checksum="91B8A3783E3756A9" modified="2022-10-12" version="1">MIKNNGTNFNIKYCYQYPWFQVQDQLVNILMLMLNFQSSNQHYGSNSQEPWLIKKAQDWVQLFQSFQFIKLLNIVVNQATNIIFFLKLKKNDSQLLFMFLNCYQSPIFEVYNSKDAKNSNGNKEKLKHYSKLIDLQSSVYEYNSVLKTYLLMTKQVKLPHGKDSELSLVHPIIGIQGQFKDSIHLELFVVDTKSTRRRIILNSLKKSECKPFFISYPLQSQSIYNNWVTILIHMPSFISQFKGSSYRSLESILIQSSCYIRRIFTMQNYNEQLLSQFQYPIESNPKSFLISFQNEQELFVDNNQEQQLNANALKQAKLKQIQYDIPKVQKLTLNPQKIQEHQQKNSSTECETKIKSKLQKQEKQNKENEFLDLSLNVDKKQKILENDEIDLKNIDELIKIIEQDNNQQKTKEIEKNKEQNEEGDLLNIMNAINAIENQGSSKQEITIKNPIGCTTITKQGNTTLILENRKEQENFVKIIYDPILNCYFDPTTNKCYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TVI7</accession>
    <name>A0A8S1TVI7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD domain, G-beta repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0280159</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8156158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TVI7"/>
    <dbReference type="OrthoDB" id="63070at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="239"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="288"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="334"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8156158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="518" mass="60942" checksum="CD4E4C43A418429F" modified="2022-10-12" version="1">MIHTTESMKCEQHNQNIDFVLIDDQFIGEEKLLCQICFKQISGLKKVYRISEAINILQEKKDSEFSEQKTIILDQLHKINNHIDTIVNFQGSLQQLINNILEALQNWKQELENRIYDISQYNFLQELNKLDNQDNTFINNQKIFEFIEIKRLQYSEKIKNNIQNLQVLATRNQGLCQLLDITHIQKIISIKLNQTVFQQDKVLSLAFNYNDSMIASSCNKEIKLQKFQDGKIGDVIDTLNEHTQIIYTIIFSKSKNWLFSAGKDQSIILWKPVRLLFNFTITNKQQYYNAHKDSILQLVLNQKENQLISCSSDCKISIWTVNYKQNSIQYQYSLERHTGPVISVCLNQSNNLLVSSGLDKQIIIWSQKNDQEWIFKQVIDKSTNDFGFRISFISDQQIVWQSFKRGLTHIFQEDNGIFKEITEQRLQLAMNAQNDGDYSFPSIFDTKKQILIQKYHQHIYFLNLNSQNKLNVQQEQIVLNDEQSYGNLSNCGKYLAIWCNQKFSIYEIDYVWSLKPPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TVW8</accession>
    <name>A0A8S1TVW8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0260357</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8155189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TVW8"/>
    <dbReference type="OrthoDB" id="10330961at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8155189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="99" mass="11496" checksum="E42D92B8943354D1" modified="2022-10-12" version="1">MNDTIVKEIMPTKISDKEKKIIEYLVEELLLEQFTDPLLKQMNFELSKKKTIFSFRSMDSDTEMSEATSCSTIKSSKLQICKSPLFRRRLSALVLEQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TWP6</accession>
    <name>A0A8S1TWP6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0280071</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8155982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TWP6"/>
    <dbReference type="OrthoDB" id="302866at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8155982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="196" mass="23135" checksum="0342037D75D58885" modified="2022-10-12" version="1">MQSPFKRQPKSIKQIIKDLILKEIEQESKKKINIIVIPTRIGQSCWGTRNLRRISPSNHYKIDQGAIFSPINCSQISPVNQSQGSPSNYRIKNRSKYEKLLKMNNQQQPNQVNKKELKFKTEPRQKSQSVNTTKPFHQRLPSLPRRINYFAEETKSLQLKLTDTIEGYQVKQDDMSFEIDEYVQMKYIKQIKHQEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TXE2</accession>
    <name>A0A8S1TXE2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0290257</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TXE2"/>
    <dbReference type="OrthoDB" id="312711at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8156938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="206" mass="24632" checksum="89A1036780B2530C" modified="2022-10-12" version="1">MPRSYSCHKRRLSEISNRQNNNDKNYIKIIESSRRNKFLDSRRKDIIKQQEIISENNRLLNKIVNIKSSFYCQSSRQSISSDCSKLSARSIEQKQMNVQRIKNNYLIKQKRFQIKATYQTIPIERTSRDQIKKPIYQIESIKQSQKCLQVQKINQLRLLSLSTLRNNNRVRISNSENIRIVNTLPEIKNKNYQIIARRLKDKLYNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U125</accession>
    <name>A0A8S1U125_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0330200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U125"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001728">
        <property type="entry name" value="Ras related protein1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8159541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="283" mass="33924" checksum="BB49EB304AAF6C80" modified="2022-10-12" version="1">MSYHYLFKFIIIGDTSVGKSCILYQFLEQKFRVTHEMTVGVEFGAKILDLENKQIKLQIWDTAGQETFKSITRQYYRSAAGAILVYDITKRESFDNVREWIKECQTHGTQDMVIVLVGNKIDLEKQYNIHNINGVKFPNQKDSNQRMKKIYYLLKQVQRIIQILLIHSHKQHNKCQKLYRIVNKRMNYQESELDNLLIKHKINKIVLNHVDVAEIIIKLYIVIAQKLQNQKQMNILSSYLFKYNYFKDLQIKQFIDQDKTHIKIKIIKDYYHKRIKINQFLDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U1T2</accession>
    <name>A0A8S1U1T2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0310089</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8157962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U1T2"/>
    <dbReference type="OrthoDB" id="292721at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039918">
        <property type="entry name" value="PPP4R4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21467">
        <property type="entry name" value="PROTEIN PHOSPHATASE 4 REGULATORY SUBUNIT 4 PPP4R4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21467:SF0">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 4 REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="627"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="664"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="673"/>
            <end position="688"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8157962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="688" mass="82173" checksum="5ADD6D3EB99CFB54" modified="2022-10-12" version="1">MDSINERCTNSIKSNEEIEKLCTPEYNNFIDKAIYLLNNGQTFQKLFVCQNIHQATEIDFYIPELISLMINKVKSEESQVIKELIKALSIIITLLKENVLSKIKEQFLKQLQELCFNYYNTPFCDQICELMQTLSKFMEFDFEPYLLILNEDSVRYEGLKIIQMLYSFMTNAQQTQSLKHVKQCISRKNERMCRIIDQVILKHDKLLMILIRDQDWLKNTISLISDFDKQVRLNGCKLFLTSWIKMEEQGYDNHYKFQDTLQDIIQFIDDDSKIIILLAQCISTIKTESIQQAFMDYIRPQPIQIELVKDYYQYINQFDSKIRQQYDDFLINQVSNLIDSSKEFSFLKHLSDLYTQVNEQTHQQLLGLVFGNFELNQIFFIPYLLDIINKGNRIKNRQLNVQLQRTFDFLKKSIFHYEQKGLWYEVEETLNILECLANNLNLKSEFNYLCFSYLQMGSQHIRKKISTYLIDQITENYDKIVPQYLQYLNHTNYTYRVLLIEFCIQICRKRSRNFFKQCNLIQVLQLQNDPVYIVRLKFVQLIYEMRLLFWNDEKDLVIKLQQIFQQFLTDKKIIVQQLAKEINQKLQQIHFHHPETIIKQDHANNLKENMENFQTKHQIRLKTPIKNNPTARLHTPTNKVSSQTPTLKKQQILFSKPQTKSTCVQLPKVPPKQNISKTQSFQKNSKDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U2W2</accession>
    <name>A0A8S1U2W2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">cGMP-dependent protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PPENT_87.1.T0310296</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.12" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U2W2"/>
    <dbReference type="OrthoDB" id="290584at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030553">
        <property type="term" value="F:cGMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004692">
        <property type="term" value="F:cGMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000068">
        <property type="entry name" value="cGMP-dependent protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000123">
        <property type="entry name" value="cGMP-dependent protein kinase 14-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000089">
        <property type="entry name" value="cGMP-dependent protein kinase 5-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0140">cGMP</keyword>
    <keyword evidence="12" id="KW-0142">cGMP-binding</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="18">
        <location>
            <begin position="73"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="18">
        <location>
            <begin position="191"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="18">
        <location>
            <begin position="311"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="13"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAD8158352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="520" mass="59550" checksum="76B7E2C8780FAE07" modified="2022-10-12" version="1">MDPKSQNNSDNQIHCDGEEDQRSGGDENDKLQNFKHNINEGGEVIHENVKRIDMKKTLNDTTFILTCLKNHFVFYNLSEAELENIVNKMFYCEAAAGTYIFKQQDHATCFFILQRGSLEVIVNEKAKRELKTGDGFGELALLYNAPRSASVKCFENCNLWGIDRNTFRRAVEEMITKEYEENRKFMEVVRFFHNLTNEQKDAIAAVLIVQKFYKNQIIVNEGDPGSSFYIIKEGTVSVLKGNKEVRKLYKGDSFGEQALYYNTVRQMTVRAEDDVKCLALGRDSLTKILGDQVHVVTFRNLQKWAFEKNALLSKLTKAQIDKVLDVMKISSCKAGDVILKKGTQANQKIIVIIEGSLKKSKSGITVATKAQAWGEEYFLQTNKAKILDDDIVMETDGVIAEITADNFIDCIGGELEEVIKKNEKILEKKLQKSDQTKKKEAQNIKKSELLHIKTIAYGQFGPVYLVKAKYNQQLYVLKAFNKNQINEQTLEKQYLMLFLCTYNKRNKYQKQSISHLLYPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U629</accession>
    <name>A0A8S1U629_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0340149</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8160094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U629"/>
    <dbReference type="OrthoDB" id="192611at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003923">
        <property type="term" value="F:GPI-anchor transamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028361">
        <property type="entry name" value="GPI_transamidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001096">
        <property type="entry name" value="Peptidase_C13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48067">
        <property type="entry name" value="GPI-ANCHOR TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48067:SF1">
        <property type="entry name" value="GPI-ANCHOR TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01650">
        <property type="entry name" value="Peptidase_C13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8160094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="251" mass="29383" checksum="3F1749C0FDFF318D" modified="2022-10-12" version="1">MIPEDTACNRKNNVPGVACAYDGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQSRRLTLSKEQKLLMFMNGHGGDGYTKMQDTTYLLDFEMEKITKEMEFLEIYQEAFMISDSCGAITLFETVKAKNMILLGSSSLGEKAYSHGRCSILSIPKTDKFSLTTHYWLKNELDKKPNLTLLDQMKKYDYAYHKANSKLIINIENKEAKDIKAADYWKPKQPILNQIILKNDQDILAQYEFLELMKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U7Q9</accession>
    <name>A0A8S1U7Q9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Potassium channel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0350198</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U7Q9"/>
    <dbReference type="OrthoDB" id="421226at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="220"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="293"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="352"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="396"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="3">
        <location>
            <begin position="386"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8160840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1076" mass="127280" checksum="B1692DC4DE1185EF" modified="2022-10-12" version="1">MNQIQQDTEGPISKLNQLMLTDEPIGEIQDFLNYSLSDGYFSIQGSKVNTNNNIPSQSSKHKGNETKRVTFDSSINSPTTKFLMNRKSQKSSDPQQLQHQQQQIFSVIQFCFIKRFINRISWKKKINSQFNLYQYNIIQDLGSSFNLKLFRDSALNLKPMISQFSQSSEGLQQIIKKKELSKKIKKQITHLKTCKDNLIKKIQQEMEKIPLITPESRLKIVWDCVVMLSRLYFLFTIPIDLAWNKYKIIYGELYIPTILMILLLVFDFILSFNSSFYQFGQIVSNRATIAKNVISKSYGLEAISILILIIYAIISNSTQSQFNLNIPKLISQIEETLNLSKPSSSLLELFKLLLVLFFVLHCYSCLWYFVGYYSYLYSEKGSWLEFYHVEHETWQVQYLYSFYFSTVTMFTIGYGDVVPISYLERVIAILYMMICSIQLSYSVSTVGAIIDTISAYGQEKMRKMRKINSYMQNRKIEYQLQYQIREYLNYYWESQNQVENDELNEIINQLSENLKEKLMNQSNSLILNECPLFQHNFSDALKSKLVNKIKQAVIQPENIINFDSLFPQLPPVFKVGKGSSLGIISFISGKQSQERFRSVGFSKLLLLSRDDFLKVIQDFPEDYERFRNLYDSLQLEDLSVLQMKCFSCNSKNHRVLQCPLLHYIPDKELILKRYCYSELQNRNSKYERNPFRQRGYFAARFDQEVIEQEANSFNNNNWKWAEFYEEPEEDQLKKSDIQQQQSIGPILEQSTLNLQAQLQQSQNIINTIQQVSDQSSKINLEPPQIKKKATLFKQRTKTIELLDIDDKQLKASLMSKARNQNIFNNNLMIIKEEDNQENSPKEKQSQSRASFSLDLYRKKPKESIQKMKKVVSMITNMNRMKKRQKLKKETKSIVNQFLEVIQSQQFLKAIQSKVRIRIKQIKDGKKQILQEKDIQDTMLLEIKLKMQIEQLNEYQKKDQELVMESYKKYKYYQIQNNLDSILEKIYFYKQQYTLNNELKNYQSKLLKYMIYPEIFFEKYRYQQVIIPKFEIEESRRGSEQDFENTLKRSRMLKKSFRNLKLSQIRPMSDGTGKFQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U9H4</accession>
    <name>A0A8S1U9H4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0370043</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8161821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U9H4"/>
    <dbReference type="OrthoDB" id="310386at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="40"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="74"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8161821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="122" mass="14189" checksum="305695F8B948B01E" modified="2022-10-12" version="1">MVIELTKNGDFIISGNLDLIKIQEYNYKSEDKNEYNIKEAERLLNQGVASYNLYKYKEAIECYDKAISINPKYDAAWSQKGYALHQLQKYNDAISCYDQALSIHINTLRLQRKGLKLIQIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UAZ0</accession>
    <name>A0A8S1UAZ0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0370159</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8162018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UAZ0"/>
    <dbReference type="OrthoDB" id="305358at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="227"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="381"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="56"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="151"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="412"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="557"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="386"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8162018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="645" mass="76885" checksum="F1BA3F87970F6AA6" modified="2022-10-12" version="1">MKQQIKQEESAQPMIILQPTKYKFMIFMDTSLQKDVDKLYNKQTRQIIDKERVEKISSVKDNVKGLREIINDYQTQQNEIDSLKLQLQNQIKAILSKEQIIKNIEDTYMQQMEEIKNNYEQRQFDLVKECEQLKFRNKELEEINIKYLSQNDLLLKTNNQLQQRVNELQKKLTLAEKNNLVTSLELEQMKEIRAGALFIKGAYNILKQQYQDNNIYTLLTKKYKSTILQFLEPKDIFTLMLTSKQMYFIIQNNRSLLNYIVNYKTLVLKNEIKYLRVESNYFYDMLYQIPEEIIQIGIAKFLVFKFNVGQYMNEIFNDAYELIEGKFQNQQQIQQKQQLSQNDSIYQNEDLLGLGETEELQTQSKQQTSTQSQTNLFTKIKSQTTNNHVQSTNKNKNNNITQSQSNQQAKSLSDLSLQLQQEYKDIDQLILESLQVQFLKAQIIQTRREMVDLVKKSQSINQQLFQTGRKCIPTPTYQPKFTIQDFMQALQMTFSKFVVHGNYLHLESQQLQQLCNYFSRLLLQQNKQIYQLKSIIDDQKVELDSQKEMKYYIQDRAKRFEERIIKAEEQIFTLNKQNFEQKQQIQQLQKKEIELENLNKEKDQKTQTLFQAVKQIKIEKDQVEIKLNQVITNFKNMKNALNKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UF84</accession>
    <name>A0A8S1UF84_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0380202</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UF84"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000683">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="105"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="393"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="412"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="432"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8162713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="446" mass="52062" checksum="3AEA7F8E05FAF7CE" modified="2022-10-12" version="1">MNSYQYDNALFTVQVARKHFFSDKFYQLYLFQDEIVMTDNTLRQPKYTIKLNLTTTINWIVQDNKILAFSFTYKNTVKPFYAPKLQLLKEMIAGRVFYTSINEFYQIQMLVGNGMSGEVYRCVSNAGEYFAVKKCEKMKLASHEGGIPQLIHELSLLQSLQHPNILKLKEVYTDESYYYIVTEFIEGRALSTELQSRPYGLSVAETIKIMLQILDALIYISERGIMHRDINPHNIMKSDPIKLIDFGLARKIKNQLIFPTSGTPGYIAPEIINFNKDKLYDDRADVYSLGCVLYKLLTGENLFHKQKNIFQENKEGIYELRKNYQHPEIHTTKMEQLFILLPYMLENDPNNRMTAKVCKIVIQEIDNNNQQIEKLIKKLVIFKYFQLSTEETHNENKTPHSISSHSHKNKKQSIDITKKSDDISIKGHKKQQQTMGNLNSSFYTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ULZ2</accession>
    <name>A0A8S1ULZ2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0420206</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ULZ2"/>
    <dbReference type="OrthoDB" id="294349at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8165424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="221" mass="25317" checksum="1E0E2205A4F144B2" modified="2022-10-12" version="1">MEISNINDLSELIKEKQKVYTFRKLELKDAKDIQLLMTKSLVSDNEGMKILQVTEEDLNTEFNLKQYQWIIQENLSFGAFNGNQLVSACLTYDLSSNNDLYYEGPEPSEAMSEIQELVGFLLGEYSYTKSMEEKEIAYLSHLATKADHFKKHLALSCAYLSVEECRKLGFIQMICKASHIGTQKSISKIFKKFEKFKEINELKGQPVDIVSLIGTLSQQQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UME2</accession>
    <name>A0A8S1UME2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD-40 repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0390334</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UME2"/>
    <dbReference type="OrthoDB" id="10266330at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25173">
        <property type="entry name" value="Beta-prop_WDR3_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="395"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="437"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="479"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="521"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="582"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="624"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="666"/>
            <end position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8163656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="786" mass="89074" checksum="57BAA0AE65A4E11D" modified="2022-10-12" version="1">MNCTYHIRNPITFVCIAPHKCQCQRKLCAECQYGHGVDLKYTVPIEIYQEMAIKKLNDSKTCELTKQRTGFKSLLSQTEQMLKKILEDLSQSMIEVYNRIEQEYQSCLNFINKNTNLAESSYTELEKLVNIVEGTTLTDSNATKNFYMMELDKMMIWWDHHLKAFIEKANLRIKQIQSLIKIQPEEEDEAYQMKEDIFEIFTQIQDIDEIIYEKIIEILQRQKVSDILEFLSKTNKQSFDESLINNQENIRDVRKQIVRIKNVLRNIQDHQFNKDDYSSETYEKTRQDIINKIKDNKGIIDFIKVLVLLTSIDVKFIQCGSNALNLLVGMKVDIRNQSFENIRIKNTSLIGGNFLGCNLSGSEFDNVDISGFNLNCSQLFNCKWKNLKIHKLNKLDGHSGSVLSVQISPDKNILASSSSDKSIRLWDVKTGQQKAKLDGHRSFVVSVCFSPDGNTLASGSDDNSIRLWDVKTGLQKAKLDGHTNCINSVCFFPDGNTLASGSWDKSIRLWDVKTGQQKAKLDGHSDYIYSVSFSPDGNTLASGGKDKSILLWDIKTGLQKAKLDDNSIRLWDVKTRLSKAKLDGHTSSVYSVCFSPDGNTLASGSYDNSIRLWDVKTGLQKAKLDGHSNFVMSVCFSSDGDKLASGSFDMSIRLWDVKIGQQQAKLDGNSYAVNSVCFSPDGSTLASLSDDKQIRLWDVLTGQEIQSSNKNYKHLLTQFNTSIFQNNPIPESAYTNITILLISQQPIFQATGALILKREFINQSGVDLKPLLTQKGSYFLESLQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V1Z4</accession>
    <name>A0A8S1V1Z4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0530260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V1Z4"/>
    <dbReference type="OrthoDB" id="10299596at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="230"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8170814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="490" mass="59870" checksum="69A5953BF63BE5DC" modified="2022-10-12" version="1">MNQNYEKYDENFQMIQKLEERKIKQIFCDNLALSTPTLMNTQRKQTDIQYIHKERAILDVRLTNVIQLDLCQFHYIEVPLFGHKKPLVIYFQFTDQAKMKFYISSHTITPNRFNNEESFERKQILRYTEQGEQVLFSQRCLYLAIFSQQATTIYAKASFGIKPQQQQKMETESKLRPSTGYQFTRSSQFFFTRKPSKDMISLNKNIEQYQPEKIKLQQNLRRVQSARKISETLHKKRQIFEEKKETLKERFEFQEKKKILKEIILYRNNIDNFIKFQQKLWLQNLYFIHMVSIIKKRYYDNIIFQFKQSIKKRRQYRVRQVLQRMLQKSGKDIFQRTLFQGLLILRMLSKVKENGAKKKAKRIIHTFMMSYGQIGEIFQKTYQFKRRMRKIIEVYRIYRKKVQAYVNRIIMLWNKYWNQIYMDIKKEDQEKVKQIKVQMQKTIINYHIEEEIAKSPYLDINQEFIYKKMESIKNQSKIIQILNFDGNLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V2C5</accession>
    <name>A0A8S1V2C5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0530184</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V2C5"/>
    <dbReference type="OrthoDB" id="286346at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8170677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="129" mass="15390" checksum="305C2234B3C94C69" modified="2022-10-12" version="1">MLNKALTYCLQDDDPIRISELIGIREYQKQPKLCQSTFVTSSDLKQMIINQKRQNSAQSYRKIQHYDGDLKPINKTFDCRLPLNQNSCRKYMNLKLVQEPTKPKLYFGIKHQQFKIKIKMQSKRLEDMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V4M2</accession>
    <name>A0A8S1V4M2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0550120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V4M2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8171627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="421" mass="50224" checksum="0D140E667EB136B5" modified="2022-10-12" version="1">MNVKFDSPKPEEASSPSIQQSENEEDSTFPLTKTIKNMLYVYGDQRNSEVHNLAHLQIQELISDIFQPQILHALYLPAPKPWEQIGSDIKFEFPPPLTKNKRILQNYLNCVFKEFVSLFQKLSRISKRLKKHTSEVNQDNNVDYETALRNKAQKEKIHIYEEQYSQQEDSISNSSITSESAHEAWEEYMKARVEQMPSQEFIKFMELRSQTFIHHKRFKQFIDPHNYGQNFIDCINLKFLNYCLCRVIKRTIQKVMQTEQPQKQVFQLSEEHLKQFGYQIIETYKIRAKKYALQDQKLNQMAYKLFLQEFCSQGYEAYFQSKWVLKKSGQLSSNQEVRAQEEWDKMGHIKQKWIDRVKAEKQVRQKLVKQKKVIPNTHYSRILLQKVGNKSYEQAKSEFSIIFKEWFIHLETITEINSIYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V4Q9</accession>
    <name>A0A8S1V4Q9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0570098</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V4Q9"/>
    <dbReference type="OrthoDB" id="297496at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="178"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="312"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="364"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="393"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="3">
        <location>
            <begin position="496"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="708"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="730"/>
            <end position="740"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8172448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="903" mass="106469" checksum="95472474E175B731" modified="2022-10-12" version="1">MSRFYNSNLDEILSSRIIIEGATPQVNPKNLSNSEIQFQSEISNIESMRSINQEIKNKNKLIQKNSQIFSLKFQQPALVNSKKQIQHSLFKIENSQDGKLKQVLNKQMLKKFKDNLLQNAHILSHNLLLEKKSLLDYLFLDYALQDGRQIKKNNIISNSREKLLFFQVYLPQDKFVKYWNLFSILFSFFILWFSPFIISFQLLHKSSVQQTIVFILIFLLIDGLITCNKAYLKQGELVINRKQIINNYLRNQVLSDFLNLLIWILMYNGIEQFQIIEMLSIFQIIIIINSLYKKINSFVDYLYLNGKLSEVLDLIFLIISLYYFVHIIGCYWHYLAVLGEQLNEISWIQKYSLEDQSYLIKYDYAVYWATMTMVTVGYGDITASNPIEIVFSNFTMFISSFVFAYSMNSIGIIIKNLYDERNLCKQLILINAYMKNNLVDDSISNRVRNYLKNQVDHESKNNQMEAQQIIDNLPHGLRDDINTNIKTRILQNMKLLVNNFSKQTQFQIIHKLEQINFIPNDVIYDPETLNIDQYLYYLDQGTVSLVEQRTQKQIQAFKEGDTLGEHSFINGLTQKFKLISTSFCQLYRISRQDFLRVLQNNQKDKEIFYSLQDQLLYLSDYSLINKKCNLCNKFDHQNCDCFIISYKPNLEKLVKMSEFLFQSRSQFNRNQRQKLKVINSIQGIQSTILQFQQCEITTNQNESNTIISGTKRIKQFSTQRQSDNKIEQPELSNPSDQSPDVQIDKMTHRKPSSNFTGKFNMQNLKSMDNKDAQRKISIAILKEQTLNPEYTSQQIIQLRSIKNFSNTAILEPEDNNKLIKFINLDIDKIYEFQNYLPQNNFQSVILNYNKQKLKFIPVYSKNMNRSLKYTFYYKIAKLACQMRMISAPITRRLLKCHQIKKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V4V7</accession>
    <name>A0A8S1V4V7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0570185</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V4V7"/>
    <dbReference type="OrthoDB" id="291466at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8172618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="212" mass="24217" checksum="51369041EB0D5F83" modified="2022-10-12" version="1">MKSEQVLNLFCCEKKQMNTLQNCNNTARIGSNNRKTSIQLKDFRGGKKDSILLQKSQVANPNVILQTCKSSKTLITQKSDEHLKITQSGVQRKSVKEIDGQQNLKQGDTYKTISPKVVINNDKINVYFVKEVTKYHINTETKSTKEETMSTENTLILQEIQQQRVYLKTSQKQTTAPVNTQIITNIPKSMLSNHKSFINQSTKKLQDNKTHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VHS4</accession>
    <name>A0A8S1VHS4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0640165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8176093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VHS4"/>
    <dbReference type="OrthoDB" id="299587at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="319"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="319"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8176093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="367" mass="43863" checksum="4D8A735E40FF6260" modified="2022-10-12" version="1">MKNFLEKHINNDYSIRMNNVSQNHEYQLNQQKCSSPMIFGNRFASPSPFRSVTPIQGQLSPANQSRRESAFSRNSDNYDFSVKEKSKIVNKAIIQLFQKQQEYQNEEECTIDYFFDKEQIILEEQELLHAKLRIIRDDFNFKIQQLEDQYLRERQSILQNLSISIKEEQDRFNEQSKQNQRKNKQNEIHTRTDSLNKHDSKGDSLPIQQEILRIGSSMKEQRIDSNLEKRDGFDIRRESRHESINGYSSHHRRESSYKTVKLRESRVKREESLKCDSVKDEQPLKSALKKKDSNRQTYNCQDDSQDDWKFIKEAKNNLEKQKTQLDDKQSPNKVRFVNQEKSQKKQQEKVVKSKYLNDIDIFLKCRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VJP4</accession>
    <name>A0A8S1VJP4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0660110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000066">
        <property type="protein sequence ID" value="CAD8176991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VJP4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8176991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="117" mass="14352" checksum="AFD359E03F6D5BE0" modified="2022-10-12" version="1">MMINGNEQNQQIYERIQLQIEYLFEGVRKNLYGNGLELKIFDRMKQIQIKIYRITKIVFINIYDKEGVQILRDIFISYQFEEFFQNCTHNLKYIITNTEQNKEGFYAKLKRYLQFNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VLI8</accession>
    <name>A0A8S1VLI8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0670025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000067">
        <property type="protein sequence ID" value="CAD8177105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VLI8"/>
    <dbReference type="OrthoDB" id="308476at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051291">
        <property type="entry name" value="CIMAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580:SF28">
        <property type="entry name" value="BOREALIN N-TERMINAL DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580">
        <property type="entry name" value="SHIPPO-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8177105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="285" mass="31131" checksum="6F720B66EFAA6408" modified="2022-10-12" version="1">MSITVSTCQQICGSPLNNSTAKNLWTFSKSDRFGNLANPVNCGKAFYDLPSQIEKRSAGIGKGTKTDFTKVAFATPSPQQYNLGSDVETNLKKQKGNKFGMSREKMASTGILGNLNLKTPAPGTYDLGSTLSDIRYTMRQRPKTNFMVLTSKEIPGPGTYESLPAVNAVGKYPISKYNNSCATLFNPKNSKRFVKDFSTNLYAPGPGTYPIDKTGIQKDGNYFISKFHSSNVRSFPKESRRTGSVGKAGTPAPGSYRLPSEFGYYESRHKANRSAGDMGENKAQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VLQ5</accession>
    <name>A0A8S1VLQ5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0670055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000067">
        <property type="protein sequence ID" value="CAD8177165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VLQ5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="389"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8177165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="535" mass="63184" checksum="322CD531A3B4BB72" modified="2022-10-12" version="1">MGNSRLQAFRNLKMRSQIISLDIIILLLVLSIAGTAIYIEIKIFYYISERSLQNTLVNQDIKQISLTTHLMKHYIKEKHQISILLVDHISSFLYHQTKFQGQVKFANPNDLCLKEDDYIKSIYLKHTQKMCYSIYGNIELEEAMQKSSQILSLYNGLKLLENFGLELNKFYPDFLQFVDVTNISFNSFYPFGLHVPNYDLQNRPWYIDHIKKANENPNQYYFFTDVYKMIVGDYNYYFSITQSLFDENKNISGILKVMLTVDDMNLDHVKSNILLINKYGQVIYNQMEILQINNSQVFYINNETITGFNDSDWKIIEDNALSNSKKSLSQHDDFSNCIILYNKQFKSYVHLRSEKFTKENFTLIIYTNISSKKILEQQFKEAFIESENWILSAVFQIILLGALSFSISICFIKIICRPLIEINRSISNHVLSVGNNINQMIFKILQQQKKRSSNLFSNLNDQIWSLYETLQIKQQKKCEQCILIEKMFYNRRDNNLHIIPIKIAIKNIPENIDQDYEECYQLLQQMIQQFKVQEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VUD8</accession>
    <name>A0A8S1VUD8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0730029</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000073">
        <property type="protein sequence ID" value="CAD8179853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VUD8"/>
    <dbReference type="OrthoDB" id="306232at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="85"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8179853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="107" mass="13011" checksum="BD5786196E738758" modified="2022-10-12" version="1">MDKESSRNTFEKKFQHITKKRVLAYQKLINDQNFVKLQGETEFIEGVKQKPQMYEDGQKEFNQKYSKQYNQIQTLYKKCIQNKKNNNQRNKQTKQNIKFNINKDLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VV73</accession>
    <name>A0A8S1VV73_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0730147</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000073">
        <property type="protein sequence ID" value="CAD8180085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VV73"/>
    <dbReference type="OrthoDB" id="27923at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14824">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45806">
        <property type="entry name" value="SYNAPTOBREVIN HOMOLOG YKT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45806:SF1">
        <property type="entry name" value="SYNAPTOBREVIN HOMOLOG YKT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13774">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01270">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Longin" evidence="4">
        <location>
            <begin position="65"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="5">
        <location>
            <begin position="141"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8180085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="202" mass="23646" checksum="6073A69E4E9E599A" modified="2022-10-12" version="1">MRIIAMYLLKYHPENPIFISQNYELSFVNWFQRPFFKETLNFGARTCAARAKPGTRNAITNQEAHAVSYICIDKNNLVALVIADDQYPEKVAFMVIQNMYQEYYRQYNSMFLDTINADQNINIPKFEEFIKLYQDPKEVDKLLKIENTLNEVTMIVHQSLDDLLKRGETLESLMAKSKDMSSVSLDFYKNAKKTNNKCCSLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VVI6</accession>
    <name>A0A8S1VVI6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0770144</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000077">
        <property type="protein sequence ID" value="CAD8181898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="297727at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="164"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1257"/>
            <end position="1278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1431"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1557"/>
            <end position="1582"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1780"/>
            <end position="1799"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="968"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="968"/>
            <end position="987"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8181898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1838" mass="217516" checksum="6027055BB0ACC729" modified="2022-10-12" version="1">MKKKYERVNMNLNQLIMQWIRDYMNLIIQTNLSPSINNIKLTAFQILIYHLQLTHFITQQYDENIFRLIKALSEISILTSLFQDIYTINILTGVLLAILNVLPYLIILHRKIRSENQNVDIISKSILKSATKLVNYYFLYFTWLLYLPQMHYISWNIMSQNDLLLLMLALLNLLFSIVSLLISNIYFINFEFNELQLRKHFTYNNCFAQLLIIPMAILNQSQDQLMKIISRTIHGIIILILFYEAYFQLPFGFSKYSIIYNRLLVMHILVFIFTTIMYQNPEYRYSLSTLMLVTQPITQYLFQVLMQDKRLKTYHNTTNQYQELLIIEDFFEIIQATQRSKKRTIELIQKFSLHFNRCQSIKCQCKRFGASGILRWDDAVILTSCLFRIGFEKHKNDSKNLEIYSLKFLTFINKYRNNAPKSYQELKILFQKKRSYSFYFIQICLLLQYILQAQMQKDEDYNINKDTRVSNVKLQVSKSERSIVQTLYQMEQIKQNILPLLIQLSNFKIQFWKSYLAGKFVSFSDIEQQVQKLQSLKHQILHQLKIYQPIFYTNGRTFNVQFLKYSALIDLLLFNNVRKYFELEKERREILQQEKSMNTFEITNINFFKGEAISVKVCIAQGPNIGKVLNEVISPLIPKFFGFGHFDNPLEAFLDYTKGNINTLMPAWLSPVHDEMMQNYIRRGGTARIGKYFQTFAKIYDKTLIRCQVYLAHNFSKDLTDDFTMIGCLKSLEEQQPKVSVEEAKKLKNVVFKGVQHILFDVNGNILGVTQGLFKMIDRLQRIRANQNISSLKQQSVEKSKSDISSLESESYDVYDQQWSNSILTIDDFYNKVLIWMLLPFICREIEQTGIEYLMDGETPPKNRYPNLIDLDNSNNVVSNKETYLFVPEDLHLFVQQYEKALQKIIDDTRVQSNNFSSGSQYKGQKYDQESDSVSLQQAVSIFNEKLCSFFYDEHLKRHQALQFGTTRQSEMQKTSVKPQSQTQVSIPSEEDSEQLKTRAEKVYQDKYSKYIEKITPQDFNPVPVYYSVNYEEYRYKKNDVDLKQQMFLIELTVNEQLLLTTMGYKRQVRDTIKQTFQNCQSKRQLLEQQLQTEDSMSVQGNMSEQRSDHDVEVTNYLFPSHPSHYFEDIYIDSQKLDQLSSPFKQMDNAQLLSSRSDEVEIQQKLTLNSQESLVKNTEKQITNMNSKAFTKKKQQDFFNENDSKLAMDSRASQQQIQEEYQVKYSENLKLFDSNYKSTLQKIEDFINPTTFRIQKYLLYIVLFLIIGYIILLTIAVYQKYNFDDCLNLIDLMLSTQKAYSQITHGLYRIELESQFQMDQKMKQFYLQQIDLSLQNFINLQSNQLFDINQVEFSVNQFYNIEYELIIKPTLQETIQMSLAQFYKIRNTTSSNLMITQISVANLQEIGQLPHLAFNYCYDDKIKNEEQTQQLLTIYMVVIFFLVMLLQFLQIPLISRLSNDHRRLYKVVMKLQIYEVQDEIETYEQVQSIFKKSLYEWMLIDFVSETRMFESSVENMQYHNNQLTDQSQTQLLNTNNKKNKYKLYEKLKRQHINQTRYIIILLIGLFVILAYFLIIFFVIFILSQQLLANIDLLFNFKLAQSSFMNIINNMDLISYQTYEQQKFINIMSQEQFINYSQELRSDKQDQFIEFNNNFVSIISDQQLKTNLEAINEDDICLPSIGIMCNETQTYIHNPSIIQYYQHGMKYLITQINKIIESYPQFFYNNSGNNTEEHLEDFFEGIEHIIYIDYGSDILVQAYDKVVTTAQEQFDKSLSIYKETLMIFILSVGVLGLSIIWILGRLLLKMQVDSIGTCQTSLLLISPKRYLNQNIIQIAQKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VWI6</accession>
    <name>A0A8S1VWI6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0730176</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000073">
        <property type="protein sequence ID" value="CAD8180139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VWI6"/>
    <dbReference type="OrthoDB" id="305230at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="344"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="588"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8180139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1055" mass="126064" checksum="34106916E3904AA4" modified="2022-10-12" version="1">MSYNINENKQGNSTNMLISNQPINIQAHNLLSNTNMTNLLESNNQFSQIVKPNGSQILKYQELLESKISLQKNQSQQQSKIQIQSLDIRGGNQLQSKFIDTGVNLLQSRTFQGSFVEQIQDRSNIQNSNSHIITTENWTESDYAKQFEEWRDLIQQNIDFINQVENDRSQIQVSNILFQEILTIKMNIINNSFLTKLSEYENIIKPYKYQMAIKSEELYKSLEILKQTNQIPFNKYANFQFEQSQPLLKSQQFNQQFTNISLEIQNQANLINSILQQLGNQKKYIEPQQNYQQILENAFNSLIQYVRERMSQQINSLMFIHEREVFNLERQIYSQIQEADNLSITVLSDKLMIIEAQLEKIQEKKQELNNFKERLNQLMNRNVDIFSEYFDDVIILYKQFKIYHEEIKQIQQIPNQPILQNLQIKIKQQLYIASKKIWLKIKQFYFNGNKIKEFNEQEKFQQFNKYNIEVQVQDIKTFLKQLQSDYIKKGNQVPLEESKKLKQLIYKAECEYRNTDSLRLQISQLEQQDIIGYKNYIIQQIKPKFFIDFSQLYAKDFYDIIYFQYADSNILGCIKKLLQFKFPTFDWNENIEDEKQRIQIVVQRYQEVSNKLQELEKLYSKETKFIKQLINWKAELFKCINILKQYLVIKEELKGQLITLLKWPQITSNIQIINEFSVHCEQISRQANQYVQAIEQLQQMQLIQSLESLFKQWNPKVKDYLIQQCNFINQSVFSPIINEYMQIVYQFLYVCMECLNYDIDEIFCRTTTYQEFENILDGSIKLQELKNIIDTNIHNSALRYKLFEIIDKFMKNKIILDFQNLFELKLQQSKQQTNNFNPRELVKRTDLKFPIIRSDIQDNFYCKQNMDKINQIIDQKELRLIFQQFRELLNNITVIIDQDNLVADIKVNQITIIELACIFAQQLLIQNELTNIQPLIRFHQQLCKFPNTKQFQNQLINLGIVFPIEYNKYLEACRRRVQKLTQNICIEFESYDFDEISQAKLLINNNEYILKQIDFPVLYTDAKSLIFEYQPLKFNCSLNVKINGKLLKQGQTIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VXL8</accession>
    <name>A0A8S1VXL8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0780253</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VXL8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8182524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="185" mass="22290" checksum="1B3DD15E81EDCCA8" modified="2022-10-12" version="1">MKDNSIIQNQIQIILSLKLFGFTFNSRINRFEQMKIQLDKSTMKQAQIQNIRKYLFIQSKKSENLIHSRQNDIEIIWIFIIVIRFRLRIYRLESFEPYRNCNCQNKENFEILGVNLKQLDKIIFVSDNYNFDNFAFSGRFKFIKVVSASDELFYQSTKLAQNYQGYYPISIYPFLTMSQTFSCWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VYN9</accession>
    <name>A0A8S1VYN9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0780210</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VYN9"/>
    <dbReference type="OrthoDB" id="287874at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053238">
        <property type="entry name" value="RING-H2_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14155:SF627">
        <property type="entry name" value="OS06G0192800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14155">
        <property type="entry name" value="RING FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="45"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8182440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="90" mass="10774" checksum="31D7C6559A5BC853" modified="2022-10-12" version="1">MRNGLQKQESDRNYNTSKIIFPFDNIMIKKLRTQQFIIQDEKIECSICLKLIISNEIGSQIRGCKHTFHQICLQRWYNINNQCPLCRQQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W1Z4</accession>
    <name>A0A8S1W1Z4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0800116</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W1Z4"/>
    <dbReference type="OrthoDB" id="306212at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="231"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="382"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8183171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="422" mass="50104" checksum="1540731DE80EEE20" modified="2022-10-12" version="1">MLQQHPYLNQPENVANKYNKPINLSQQLSFSKSLPGQGVLPEQNYATPQWQKQMNPQLSNQQPQIQTIQSKYIPQQQTIIYQGQPTYVQQPQQQFVPIAYPQQQQFRQSSPPRIIKMNQQPQQAQPIYRQPEVRIPEYYPQPPIYYDDPPFYEEPIYYPPPQKSIKLPPPHIDNYFMPNQVSYIRNLDDGPKYPEYPFKVLDIRDYFNKALIDMATQEEKIERAKVQVCSQLDFRINGLFNQLDQNQTGQLSQEEFAVGLKAFSLQPEQCDIKLLFEEFGYQGMMDFQQFAYMIKPQTDLKNTKAYQQDDEKVLPETKLLIRNLFVTLLNAESVFESLRQYLISKEEELGQKIQYNQIFEEIFSNDHPNNLRFYLQNQGQYFDIAVINSLFNRFDRECTKRITQEQFIYAFLPKLNKDDNYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W2R6</accession>
    <name>A0A8S1W2R6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0790003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000079">
        <property type="protein sequence ID" value="CAD8182572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W2R6"/>
    <dbReference type="OrthoDB" id="300886at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8182572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="244" mass="29027" checksum="FA69FFF23A0D73D7" modified="2022-10-12" version="1">MVQLNFTNFPPEFIIFKIQEVYNMTEDICPLFGWDDPVPNEFKEIFSKYINEQYSRKCSSTLEESLIVDEDQEIIGKSQNENSICPLCYQNFQLSHIQLYSNILFQYFYVHTQCVLTCKHKTAQLALLTDQIQHAQIMEQVPLDYDLMDLNVKVFVEFKVYHSIAQQPQLNLTHSTTLKQKKNTSILLDYANLREQFGKKQQHFIRLMQGFVQIDVYIMDSNLMQESKNVHNYEFYYQCKQSFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W2W9</accession>
    <name>A0A8S1W2W9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0800036</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W2W9"/>
    <dbReference type="OrthoDB" id="284200at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8183017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="162" mass="19606" checksum="7BF142C955FD61F2" modified="2022-10-12" version="1">MKNLKLTNFEQFKKNYLDENDKIKVFKYFYQYNNCFGLISQEDMQDCQQAEFLEKARTYSAFLSMSCLMLTLDKTIFRRSSFKPTKFLFQYGVLPMMSFQITKNYFCRDVEQTFHDMTEKYQFGVEQYHQGMELMTRAHKANRLNEFLEKGVDFDWSTVENE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W3D9</accession>
    <name>A0A8S1W3D9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ABC1 atypical kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0810153</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000081">
        <property type="protein sequence ID" value="CAD8183741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W3D9"/>
    <dbReference type="OrthoDB" id="201153at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13970">
        <property type="entry name" value="ABC1_ADCK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034646">
        <property type="entry name" value="ADCK3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051409">
        <property type="entry name" value="Atypical_kinase_ADCK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43851">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43851:SF3">
        <property type="entry name" value="COENZYME Q8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="6">
        <location>
            <begin position="205"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="67"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="67"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="81"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8183741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="549" mass="63762" checksum="5A292A26BCA14D81" modified="2022-10-12" version="1">MLPFISSLIRKKEFQDFAGVCRGIYRVIDGVNQPQLIKDNLIDQFKQEKQISTLNVQRETLKKVDQQQQQSIQQQNNKLDQQNKDQNQRKSIEQDQQSNDQQSQIPSPSPINKQFTENKVPQTAIGRVYEFGSLGVSLATNAMKTMVTQKNISFRQALVSEENASILAKGLCKMRGAPLKLAQALSIQEDEVIPKHIRQAFEQARQNADIMPQKQLEKMLKQELGSDWMSKFKEFELKPFAAASIGQVHEAITTQGKRVAVKIQYPGVKEAIDSDLNNLKRLMEYTNLFPKTMFLDKLIANTRKELHEECDYKIEAAKQINYRKLFGNQPEFAIPEVFNDLSTTRILTAEYLYGDTIDFAAENYPQHLRNEIGRRVMSLTLQELFKFRTMQTDPNPSNFYFDRNNNKLILLDFGAVHEYTKPFMDNYIGVIYAAIIQDRKECLQRSVDLGFLTGEESIRMKEAHVDSIICVGEPFRYQGEFDFGEQQMTKKIYELMPVMLKYRMRPPPPEIYSLHRKLSGAYLMNMRLKTKVNCRDIFMNLYEQYIKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W9I3</accession>
    <name>A0A8S1W9I3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0880039</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8186888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W9I3"/>
    <dbReference type="OrthoDB" id="7851174at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23800">
        <property type="entry name" value="UBCc_UBE2K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000037">
        <property type="entry name" value="ubiquitin-conjugating enzyme E2 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="9">
        <location>
            <begin position="4"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="8">
        <location>
            <begin position="158"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8186888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="197" mass="22433" checksum="12D5FCE4645257E6" modified="2022-10-12" version="1">MQQVQKNRITKEIQDFEQRQKKSEDNGISILVVDNNITHWKGFINGSPDTPYEGGYFQIDIVLTADYPYKPPKMKFDTRIWHPNISSQTGAICLDILKDEWSPALSIRTALLSLQALLCDPQPDSPQDAVVANQYKSNRELFVKTAKEWTLNYASKNKQNEKIANLVLLGLDEQKVKEALLRFGYDEEQAANFLLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WFQ9</accession>
    <name>A0A8S1WFQ9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">60S ribosomal protein L23a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0930025</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000093">
        <property type="protein sequence ID" value="CAD8189084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WFQ9"/>
    <dbReference type="OrthoDB" id="1267328at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000532">
        <property type="entry name" value="50S ribosomal protein L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01369_A">
        <property type="entry name" value="Ribosomal_uL23_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_uL23-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011118">
        <property type="entry name" value="PRK14548.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11620">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L23A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8189084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="149" mass="17193" checksum="6748D8B8CCAE3DB0" modified="2022-10-12" version="1">MPKGQTTTTQKAQKAAKNTRVAKKVVRARKHFQNRFHTEKPLALSRKPKFTRLTRQLTPVSKGLDFQNVLRHPLITEKDMKKMEDENTMVFYVNQKSTKPQIKRAFQKIYDVKVRKVNILNTFGGKKKAYIRLGGENDALNLANKIGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WFW6</accession>
    <name>A0A8S1WFW6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0920189</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000092">
        <property type="protein sequence ID" value="CAD8188958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WFW6"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8188958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="131" mass="15595" checksum="306BD44DD7371D4F" modified="2022-10-12" version="1">MSSEESVMQYILNKVNSLLNNKQQISILQIICQWDQNAINISSIILIQFMFIRNVNQVVFLLSCAYFNFYIFQSIEIEDKLLKLALQNILAGQKVRNLYRFYFKDHVFIQEMTNKCQLYSQGFEIKKQIYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WIB6</accession>
    <name>A0A8S1WIB6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0910154</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000091">
        <property type="protein sequence ID" value="CAD8188465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WIB6"/>
    <dbReference type="OrthoDB" id="10623953at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8188465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="119" mass="14218" checksum="C46C91AC11D63C4D" modified="2022-10-12" version="1">MLYLFSDEFISIFQIKVQNCIVINNFVQQLFNNKSTNSQVQVIIIFMIVNRERNSKNITKQKVQKLNRISTIHIGIIEILSCNEIIINQEKILRIQSMIGVMRRFQFPEFRQNNKQNER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WL26</accession>
    <name>A0A8S1WL26_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0920128</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000092">
        <property type="protein sequence ID" value="CAD8188859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WL26"/>
    <dbReference type="OrthoDB" id="307548at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8188859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="26364" checksum="CDECBD613CD7619D" modified="2022-10-12" version="1">MSLSIFSAKTSYYKNGIWWFTKMAARVVGEERWDALRMARRVYGNKFYYGRQQVLFDVFYDWPALANMIGIYPKVDTSHGFLHWATYEAYRDWQEHTLNSDGAFAMWVYLICGVYIAHFTYSYMIPYYWTNLFPVKNEEFVRLRMKDAIASTVQEELFGNQFAEFGWAPHDFHYNRHRCMSGYSHPDDPRTMHMGSFNRKHKYKEHYMKKVGEQSRMAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WN90</accession>
    <name>A0A8S1WN90_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Vacuolar sorting receptor thioredoxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0970054</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000097">
        <property type="protein sequence ID" value="CAD8190662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WN90"/>
    <dbReference type="OrthoDB" id="293836at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056858">
        <property type="entry name" value="VSR_TRX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22702">
        <property type="entry name" value="PROTEASE-ASSOCIATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22702:SF1">
        <property type="entry name" value="PROTEASE-ASSOCIATED DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25011">
        <property type="entry name" value="VSR_TRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar sorting receptor thioredoxin-like" evidence="10">
        <location>
            <begin position="27"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8190662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="280" mass="32602" checksum="DDC93A8981C9288F" modified="2022-10-12" version="1">MLFLSILIGYCVAQLNLTIGIDITNKESLNYLGRLEQYYEQFEEKGLSIYILNHILPCYTCKQRHNYTQPEPNCFGGGRYCQFSTYANGQLVLTEILRQNCLFTKDIYIFLSYINLFQQECVENPHYTFCSDNILKNIINYNITEIDECINASFIGKGDQALLENQILAQEMNKKYNQSSLNVYLDNIDITNLQFNEMIIQLCQKLNNDPPEYCNIYSLVEKNTTIKLDEQIFFYLFIVILILLILISIKMLKKVNYVIQNKASIPIEETIGIISEENVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WNB2</accession>
    <name>A0A8S1WNB2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">P-type Ca(2+) transporter</fullName>
            <ecNumber evidence="2">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPENT_87.1.T0880205</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8187216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WNB2"/>
    <dbReference type="OrthoDB" id="3352408at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02083">
        <property type="entry name" value="P-type_ATPase_SERCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000123">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000027">
        <property type="entry name" value="Calcium-transporting ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000014">
        <property type="entry name" value="Calcium-transporting ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000028">
        <property type="entry name" value="Calcium-transporting P-type ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="270"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="307"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="852"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="931"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="962"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="998"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="15">
        <location>
            <begin position="9"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8187216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1037" mass="114519" checksum="0E48B7A6AD39AAAC" modified="2022-10-12" version="1">MAEIDLTQPFHTYPVDKVVKAVQTNLQKGLTSVEAQARLAKYGHNELEKEEKESIWEKIKEQFEDNLVRILLLAAVISFVISQFEDHEDSHAVPPWVEPCVIFTILILNAAVGIWQDLDAERAIEALKDLQSPHAIVLRDDTWGQIEAKDLVIGDVVEIKQGDRIPADLRMVDLKTITLKTDQSILTGEVNPVNKTTEPVLKDQAAVQDKINFLFSGTLVSNGTAIGIVCNTGMKTQIGKIQSEVQDAAKEKQEDDDPLSKRLDEFGDKLAKYVTYICIICWVMNIGNFSDPAYGGTIMGALYYFKVAVALAVAAIPEGLPAVITTCLALGARRMAKQKAIVRKLPKVQTLGCTTIICSDKTGTLTTNEMCVKEMVLLSGLDASTITVFPVEGTSYHPEGKIDGLDAKLVKGNGLAGNLNRLCQSMALCNESKLFTDKGRVQRSGLPTEAALKVLVEKIGQYDKSFNGKPILEAPEQYNEKIVGEFTKRATLEFTRDRKSMSVLASSNNEKGNVLFIKGAPDYLLEKSNMILNSNGDVVPLKAQDKNQLLKIVKDLAEKGLRTLAICVQEDCGQLSDYDGPKHPAHNQLIDTNNYKDLENKPIIIGVVALQDPPRPEVKRSIEKCREAGISVIMITGDIKETAQSIAMQIGILHNQSQFPTHSFTGLEFSTMGEEKQKKVLEQVIGKPSGLVFSRTDPSHKRELVKLLTGQLNQIAAMTGDGVNDAPALKQASIGIAMGISGTEVAKEASDMILADDNFATIVRAVEEGRAIYQNMKGFIRYMISSNIGEVVSIFTSSALGIPDGFNSIQLLWVNLVTDGLPATALSFNPPDPDVMQKPPRKHDEPIITEFVFVRYCVIGTYVGLATVFVFVYYYLGYEWAGDGHPIVTFHQLRNWAECHHWEGFKVANFDKYDFSKDPCLYFSWGKQKASTLSLSVLVVIEMFNALNALSEDGSLLKVGIFANPYLIIAICGSMTLHCMICYVPLFENIFNTVPLSIQDWILIIAVSAPVVLVDEVLKFFSRLRNAKLLEERKKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WRU4</accession>
    <name>A0A8S1WRU4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0980073</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000098">
        <property type="protein sequence ID" value="CAD8191085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WRU4"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000104">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000224">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF51">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase A1" evidence="6">
        <location>
            <begin position="167"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="372"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="200"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="361"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8191085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="494" mass="55630" checksum="3BE3088827B6C35B" modified="2022-10-12" version="1">MLLIAALVFLGTAKHFTVELKSRHTQEHLIEIVNMLQHSHAQLLEENHYEKIGKGIEGALQFETVMLEKNIQKKNLKHQNHHNKKQEHHHKKRKNTNMVVNESLMDVIPLTSEEKGIFEESGALSPIDIHIEQQMEHQSQKLLTTNRNQDGLYIADVPLTNIANTMFIGELQVGSAQGKNAFDVIFDTGSALTCIASEQCKDIGCQKSKRYNRAESQSFNEIGKSVEIVFGSGTLKGLINRERIRVDGLDLKDALFIEVTQQIGDAFHEGEFDGIVGLGYPHMTGVPTLFDYMIQQHKLHSNVFTFHLNRATGNSGSQLIFGGSDDSQIKGEWVYHQVHEQFYWSIMAEQIKVGNKDTGICTHQHKCKMVVDTGTTLLTGPTKDVRTLLSMIRVEPKCQNYQTMPDITFVIDGHDYILSPKDYILTITQSGVEAPYHHSSMDQIVGCAGTIFPLDLPPKQGPLWILGDVFITKYSAKFDRDKNRVGLALNKNLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WVH8</accession>
    <name>A0A8S1WVH8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1020133</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000102">
        <property type="protein sequence ID" value="CAD8192721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WVH8"/>
    <dbReference type="OrthoDB" id="302049at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8192721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="235" mass="27624" checksum="9CF4BF817FEAC38E" modified="2022-10-12" version="1">MNRNGLEKICNQATNFWKQQFIPSPKQDQDVSTNVGSNILEEQSIFEISAKKTEQYLFPDIERYHFNIEKCEQQFPQDLLQQPQQFELEDNYEAEASCSQCSDCQSKRKDKKKLKPRSCKSTKQRFTISEEAFIMEQTRKGTSFTDIAQQIPGSTVNQVKHHLLKKLKICSKNTDLQGDNIAEIILKIQQLQQNENIDNAVKIQELRQHISFIENHLHHTKQMILQKYMMLFQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WW47</accession>
    <name>A0A8S1WW47_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1060107</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WW47"/>
    <dbReference type="OrthoDB" id="25131at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="112"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8194264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="313" mass="36286" checksum="C1D22594E42BD184" modified="2022-10-12" version="1">MNLIQKYSDLQNSLELLNNEPQQLNSNDIYEYVAIDISPNFEWLAAGGLYIDNIVLWKISCQSQTKVIGTQMNEDLGQLEFSNDSVFLAACGSSTKIIIWNMKDLDENPAILDGHQSRIWRVQFFNQPLFDILCSGSSDASVKIWNVTNRQLLQSFNMNEDNPIYSIGVSVKNEWIIMTGNDTRALILDSKRLKILSQQKICENQLYQVRFPQHTSQYYACSGMDEYVRLLSKNKLIRKLKFPENWKWQIEFTCQSILAISSINSIQFWDISRGIQLKVVENECYTYPSFRFKYLEKGICATASKSMIKLWIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WWI6</accession>
    <name>A0A8S1WWI6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1020021</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000102">
        <property type="protein sequence ID" value="CAD8192529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WWI6"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8192529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="209" mass="24621" checksum="AF0C96FD694E7C84" modified="2022-10-12" version="1">MNTQSIVDQLKNKVEECLKGVQQQLDSSFNIIEAFLDQLPLAHHHLFKSLYHPTNIHQLTNSRSKTYNKFQYSLKSQVHSSLTLIITNKKIENNRKHNNQHQLNLKTFTYNLLQDKSINQSEYCRAITINKDNTIVLVGCNNENTGDVLTLNFIKKQIQFLFGNRYGQIIIQFMNQNSQWECQQILNSHYNYINCLIINNNDDFYHIRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WXJ8</accession>
    <name>A0A8S1WXJ8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SURF1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1060050</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Probably involved in the biogenesis of the COX complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Mitochondrion inner membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SURF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WXJ8"/>
    <dbReference type="OrthoDB" id="311610at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002994">
        <property type="entry name" value="Surf1/Shy1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045214">
        <property type="entry name" value="Surf1/Surf4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23427">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23427:SF2">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02104">
        <property type="entry name" value="SURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8194152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="301" mass="35372" checksum="BA895965C29CAA43" modified="2022-10-12" version="1">MLSTQVFNSFAQRQSNGIIKLLAYGVFFGGAYGCAWHQWKGAQQKQLYEKVETEITEWKPITINGLNANRYPWARNIRQWEYKLVKLYGYFRDERFFVRREREGRDGFLVFAPFVTALQFNDTEQDPEQTTKSQVMVNLGWVPKDNISDIQMGQEPIGTTTFENVPHNEDDDQLTGFNRNVLNMEEDYQMPFVEFVGMVRRGEEEDILKGRRNWPREGVYNYIDLWFMSRLYRSFNLTDCSSAYIERLVQEYDEESANLYPIPATKDNFDKPLPTPQTHQAYSLFFGLSSIMSLALLAIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WYR2</accession>
    <name>A0A8S1WYR2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1080081</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000108">
        <property type="protein sequence ID" value="CAD8195013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WYR2"/>
    <dbReference type="OrthoDB" id="291311at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8195013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="86" mass="10110" checksum="FFD402B1B0D6F8C5" modified="2022-10-12" version="1">MQSFYKLAFQTLSSFHSLGLVSDEEKSYLKEVIINWSTPQLNTSQDQMSVLVLQIIRVLRNQVQHVYQTQKFLCLIEEETDEDENI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X2C3</accession>
    <name>A0A8S1X2C3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1080140</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000108">
        <property type="protein sequence ID" value="CAD8195131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="327"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="490"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="657"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="800"/>
            <end position="838"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8195131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1453" mass="179533" checksum="60327138D03AB71D" modified="2022-10-12" version="1">MKNDFQKKIDDNDKKNYEQLLDEQITLCQDEENCKYIFRIDNYRYDEKQNKFIINYNFKEITQNISQEAKSLSQVQLKTLEEKLNIFDQLLELAEFLKKKKIIHNNIKKNNLILYNNKLYLTDFGYLQEQDESNIFMDQKNFVRTLNQDQQRQIYPYQSYELLEILNKYYLQNEELMEKLEQRTQKDIQVNDQHVLTVMMFEIFYYFDKNYQYNLQNSLKCSKKAVRKNQLQWMKERNQDVQDYILIIIQQILKKRFIIDNELLENFPKSLSGCIFQIDREKLINIDNQKLVQEYFENKILEFDQELLYCFTYKELQQLQGQIQNPQNQFEQDLKQEIEKIQKLYDNIDNKQLNKENFQQLELEKQFICLVYLDQKIDEFDFVKNQDQSQEVKKLLDYYNLKQELKEFYTQINKKQQNFKFNRQIKIKSRIQSEPPPQQINQSLQQSQLNPLYKIDRERLINIDNQKLVQEYFEHKILEFDQQFLYYFTYQELQKLLNQIQKQQDQFEKDLKQAIEKIMENYDKIDKNNFNKENFKDLSSDEQFICLVYLDQKINEFDNIQNQDQSQEAKKLLDYYNLKQELKEFYDEVNHKQQKDFKFNSIKIKVKIQQYKINKEKLINIKNNNKQLAEEQNVNEIKKFVQQLLYYFTYKELLYILSQIQEQKNKFEKDLKQAIEKIMENYDKIDKKEFNKENFKDLSSDEQFICLVYLDQKINEFDNIQNQDQSQEAKKLLLDYYNLKQELKEFYIQINKKQQNFKFNNLINQRYQYLIDKENQNIVQEYFEHKILEFDQQFLYYFTYQELQKLLNQIQKQQDQFEKDLKQAIEKIMENYDKIDKNNFNKENFKDLSSDEQFICLVYLDQKINEFDNIQNQDQSQEAKKLLDYYNLKQELKEFYDEVNNKQQKDFKFNSIKIKLKIQQYKINKEKLINIKNNNKQLADEQIEKEILEFVQQLLYYFTYKEIKNILNQFQNPQNKFQKDLKQEIEKIQKLYDKIDKNEFNKENFKQLELEEQFICLVYLDQKIDEFDINQNQDQYQEVKKLLDYYTLKQELKEFYVEINDKQQKDFNFNNLKKQRFQYLIDEEKQNLVQEYFKHKILEFDQQLLYYFTYKEIKKILNQFQNPQNKFQKDLKLKIEKIQKFYDMIDKNEFNKENFKQLELEEKFICLVYLDQKIDEFDCDKNQDQSQEVDRLLDYYNLKYELKEFYDEVNNKQQKDFKFNNPIKIKYGNQFLYEYINQITLRLQSDKYLVQQVDLNNMILNIPNQEQQQIEFKQIKQDKNTFQFIIHKFFLQNDLYYCKYLEVKEQKRNFEPMVIMNFNLIKEYRFEDYNSKVRLIDHKKKFKYFGEYQLDEQGEIKFNGQGTKEILEKDKEILETGQVFKVKGNFKNNQLNGKSITFYHHYDESKITFSIGEYKDGQMSGQHIHYPVNDQKILKTTYHWKFLKYFNYYFETG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X443</accession>
    <name>A0A8S1X443_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1130136</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000113">
        <property type="protein sequence ID" value="CAD8196773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X443"/>
    <dbReference type="OrthoDB" id="1305at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8196773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="421" mass="49761" checksum="03E4ED1B06D4977C" modified="2022-10-12" version="1">MTSEYSLEWKYIPVTGSLIDQNSNKVIWDLKSEDSKWPKYLFTVSISQQKEFMSDPELGWNWHKSWNLPDINYTINFKNNDTTMRDFRGGILEVQLFVIKKGLIANQYISLGLNGNSKQALFEPSITFSGLKFGTTSYNNKHSKFNLIFVLFYSRKEEVLILDSQISSDVFVDSRNRSNHKNGEISQEMFIDLFPPLLIDQILYKRENEKQLSKFNQIDNSLQGFINYYTSPNIRNKIKHPLFMIMRFSKCIKLFLIADFIKKSQSVKQQLNQSKEMKLLINIENSNQNTLKKVLDIIKQLENNYYEICIQRSNIPSNSVEITDICSLTSEYKRKYKRFINHAENQKYNENSNTSKTQKKKLKYTQKNHQIQQNQGQINLISQNKQQNQDDEQINKEYQVKEERDLEMNQQKIKICLFTLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X4X6</accession>
    <name>A0A8S1X4X6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T1110153</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="9">Microtubule inner protein component of sperm flagellar doublet microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RIB43A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000111">
        <property type="protein sequence ID" value="CAD8196147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X4X6"/>
    <dbReference type="OrthoDB" id="308880at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008805">
        <property type="entry name" value="RIB43A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14517:SF6">
        <property type="entry name" value="RE41410P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14517">
        <property type="entry name" value="RIB43A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05914">
        <property type="entry name" value="RIB43A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="97"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="107"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8196147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="143" mass="17265" checksum="1E558C13E54F06BA" modified="2022-10-12" version="1">MKRTKETIFREHPDWQQRQGISGLKEFDGEDLQFDSRNKFNKDQQKQWIFEQIDEKKRRQEQEKAEEMAYAQQTLEINRFRGMLQDNFASRKTDMGVATKQTNLQLAKEKKDKEEREKQEKLAAERAERDLLLERGRKQPFKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X8V8</accession>
    <name>A0A8S1X8V8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1150079</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000115">
        <property type="protein sequence ID" value="CAD8197384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X8V8"/>
    <dbReference type="OrthoDB" id="302629at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="193"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8197384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="219" mass="25121" checksum="BD03909D85F4FB88" modified="2022-10-12" version="1">MGNGACHQQVPSIDEVNLVARNFKQKFEPCNSQCILPLLNSQSQSPSKPSIESKNPTIARLDEEEFEILMTNEDKYDQDLSPVRQSKLSRAQSPTKPIKSKHHYKQEEIFFLELLKQDKTNITRRKSADRQLSPVKVIHSNHQKKHNTALLIHSPDQRSNKLIKINRLSCQLDEAVILSLEPKSILKSIGSEKSLSNSQKSKTNSQKKVRFGMVYRQYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XAT8</accession>
    <name>A0A8S1XAT8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Apyrase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1170118</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GDA1/CD39 NTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000117">
        <property type="protein sequence ID" value="CAD8198129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XAT8"/>
    <dbReference type="OrthoDB" id="6372431at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017110">
        <property type="term" value="F:nucleoside diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009134">
        <property type="term" value="P:nucleoside diphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24003">
        <property type="entry name" value="ASKHA_NBD_GDA1_CD39_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000407">
        <property type="entry name" value="GDA1_CD39_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782">
        <property type="entry name" value="ADENOSINE/GUANOSINE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782:SF83">
        <property type="entry name" value="GUANOSINE-DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01150">
        <property type="entry name" value="GDA1_CD39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="191"/>
            <end position="195"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8198129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="430" mass="48901" checksum="AD211C0E55D0B5BA" modified="2022-10-12" version="1">MIQILLLLGVVIANNLRFKAKGTIPNLWNIQASICNGVMFDAGSSGTRVYVYSWNCRETQTMPYVTLTEKSIEKKVKPGLASFASKLNEIPNYLQPLIDFAITNIPKSMMAYTPIMLGATAGMRLLTIQQQTDIIKEVQRIFSKSGLLFLSEQWARVISGQEEGAYMWLSLNYLLQKMSDQEQATTIDLGGASTQISFKPKGLIEDGVMDLIIPNLNEYEIFSSSYLLYGMDQALIQVHKQFQDNDTFTITTPCYFNGYENVYEYDARFRVIGQGNYTECKKLIRQYLNNDPQNCQHSNCGINGQYQPKIDGNIIYAISGVQSMVSLFGFENYTLTQYSNKVFSFTSLVWSDIEQIPEYINNPYITTDFFSSIYVDELLSYGYGINKDQLIYSPDKIENISPSWTVAAMFYQLAQIQCQYDSPVCIQLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XAV1</accession>
    <name>A0A8S1XAV1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T1170085</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000117">
        <property type="protein sequence ID" value="CAD8198065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XAV1"/>
    <dbReference type="OrthoDB" id="307646at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF32">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="13"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8198065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="474" mass="54768" checksum="54F91E503E1ED9E4" modified="2022-10-12" version="1">MEIEKKFSQCGNYNILQTLGSGYHAKVKLGEENGKKVAIKIFKNQHDTAQNIKALTNEINILKQLNHPNLVNLIEFNNALPYRRKNGQIEQRVCIILELASGGELFEYVASSGRFAQETSRFYFKQLLSAMVYMTSKGICHRDLKLENILLDENFNLKVADFGFAKVMEAAKLKTSLGTPGYMAPEIVLKKEYNGTKVDIFSAGVILFILHAGSPPFSQAADKDQYFRLLSNNKWDLFWNQHLKYKGGNPEFFPQDFRNLMHGMLAPNPDQRFTLEQCMQHPWVNGPTASIEQINNEFRKRHERVQAEILASKMKKQAQKEKTQKGPIGNFRSIIGESEQENFTETIEKFNLNFENRFLTKGKDTGLPNDITLYQDPKFVFCYLLKNCKNFASKVNKIHETKYKINFEMADQQEENADQQEENLQYSIEILDCDDEMIKLNLTKISGDYLKFKVINGQIRKTIKEFLKEEVKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XBZ5</accession>
    <name>A0A8S1XBZ5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Radial spoke head protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1190030</name>
        <name evidence="8" type="ORF">PPENT_87.1.T1190042</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000119">
        <property type="protein sequence ID" value="CAD8198498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000119">
        <property type="protein sequence ID" value="CAD8198522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XBZ5"/>
    <dbReference type="OrthoDB" id="272202at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001534">
        <property type="term" value="C:radial spoke"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035082">
        <property type="term" value="P:axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060294">
        <property type="term" value="P:cilium movement involved in cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22963">
        <property type="entry name" value="DD_CrRSP4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006802">
        <property type="entry name" value="Radial_spoke"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13159:SF0">
        <property type="entry name" value="RADIAL SPOKE HEAD 6 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13159">
        <property type="entry name" value="RADIAL SPOKEHEAD-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04712">
        <property type="entry name" value="Radial_spoke"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="408"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="408"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8198498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8198522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="438" mass="50693" checksum="5E537A921F1AE544" modified="2022-10-12" version="1">MLKEKLQEVKDQNTNLYEHLNNVLTKILLDNPKNAFELFEDYSFQVKSNGFKFEKYNEFDNAERSKINMDQSIQQKTLKLINKINLGTDEEPQEATQTGYLPNLIEESYLYEWAGIGIDENYKIFKALTLLSVTKQASKLRFWGKIMGRQKDYYIAEGIATSQVEDGELPPEVEPRGTGINTKSYWACNDLLNDKDWIELPLITPQQINTSRLIKYMFTGNLESDVITNPYFFGKEKHLLKAQIVRITQSTVIIPKGQYILREGEPREIDPTPEYKLPGFHDLKTLNDWMHFNQNILNCGRLVHLIPSELPEGIEPEVYQKQIENADPFENRLKPISNDKNAWKIRIVGDTTDYNPLSKDTNNKVNNGVIIIKSLVWPGLVTVYSNKVLSQLYYGYGYKATNNHFYPKQPQQVQSEQPDLPEQSEPNFPAEIPQQQQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XES5</accession>
    <name>A0A8S1XES5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1210137</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000121">
        <property type="protein sequence ID" value="CAD8199353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XES5"/>
    <dbReference type="OrthoDB" id="2017893at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000134">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF519">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE MPK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="5">
        <location>
            <begin position="30"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="6">
        <location>
            <begin position="94"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8199353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="351" mass="40896" checksum="FF209F776109C5C3" modified="2022-10-12" version="1">MFCNSPMVQKSYQSSSLSYQQQMDDMSCIIDNGQTGSIYLGNIESASSLENLRRHKINGVLSICMNKIPFDVQTQLKNYQHIYLEDCESENITRHFDNSNQFIERARQNGNVLIHCMAGISRSATLVAAYLMKKSNMNAQDALKLLEQKRWQVYPNNGFLRQLQQYERVLQQQKSKSEIRDSSPLKESLLKSKQFQTPIKEISFINKYEEQQQYSGIKTRPLDDINYENYKRKSLGIQLYCSPESSQKIQFSLTNHKLNSAIKTSPDFIRKQNYLQDTIHGNKKDGLASLALELNSLDWQNRKQGFEQKFPKQQTQNKNKIFISQNKPLQFNQHQNKLDELLNAFNRKPIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XFC2</accession>
    <name>A0A8S1XFC2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PPENT_87.1.T1220033</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000122">
        <property type="protein sequence ID" value="CAD8199561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="447173at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="SMART" id="SM00382"/>
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        <source>
            <dbReference type="EMBL" id="CAD8199561.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="4268" mass="496558" checksum="202DD736E17AF9EE" modified="2022-10-12" version="1">[encoded data, 4268 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XIU1</accession>
    <name>A0A8S1XIU1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Anoctamin transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1250115</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000125">
        <property type="protein sequence ID" value="CAD8200754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Anoctamin"/>
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        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
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        <property type="entry name" value="ANOCTAMIN"/>
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    </dbReference>
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        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="801"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="826"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="899"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="984"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1107"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1188"/>
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    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="7">
        <location>
            <begin position="779"/>
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    </feature>
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        <location>
            <begin position="1"/>
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    </feature>
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        <location>
            <begin position="184"/>
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        <location>
            <begin position="1"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="23"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="203"/>
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        <location>
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    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="EMBL" id="CAD8200754.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1262" mass="148500" checksum="4620D6ECB4A07926" modified="2022-10-12" version="1">MESHNSGSRNSRTSRQSLAEVLQAHEEQQEQELKSKLKKAQTLSQKKIRQQQIQTLKEQSLREILGFFTNQIDVKRFLKNKKKLLSSDDTEQSEKYQDEFLNPNEFQEMKHIYGDCNIGDLVIVFPNPDDEEALAGEITFLEAEKVFDKFITVITDEVSKSYLKKERDSFLLSYLTLGDYVKPEKKNKKQDDKKQNKANNENKNQEQNKKEIQKEDNQNKEKQNQVDNQNNQEEAKLLQNQKDQPSKINSIQSQHSENKPQEETSYQKYQKFMKLYEVQLQLEEEQYQLQRNVAKQNQGELFSEVKFSQQFYRQWQKQKKQEDLIIDPLKELLKIDLNNIKQFKNISKQLSKGGYLVKKMNDEVDYDWICQNTPPKDFLTLVRITVINKLCRNAFVHCRQFVSTTGKHIFVVVKSHPQVITRQAQKMKITKQIEMGFADLFSLEPVDNQFRPLRLKSYVRIIDDDLRNIFAQEEYAQSNEPEFDYLNCQDVENYCLKLYIKVRQWKGKDLKYNVVKKQRDVDKILKEVVTDLNIDQSHEKVELIDDIPISDEEWQVYYLYLESILCFGKKIQWAKEQKMKEVIENIAFLYKLVFLKALGDANQIWYAENQSFFRELKGEKQIIFNLWGRLNITPVAPFTEYFKSTGSAGSSLWRKYEINENKHRSEFINMEKIKISNSIVLKHINILKMLQMQYIFAYFPIHDLHQLKGQGDKKKQFFEPLKKNGFIIDKEPTEGEKYLRDLFKELADEAESTDFDSESLVQESQFNYRIPWFVPIDSIRDYFGEKIALYFDFLSYYAKQLWYMAIVSIITQGIMYSATIELRQTAIIGFSIIIIIWSTFFMEFWKREQVYFSVRFGQQNFEADEAERPAFEGDFIRSVLNDDLNEEFYSPFKRKMKQLLALSISAIIIGCVIGCVIGINILKNTLIEQQLDQNLSNTIPSILQAVSINFFNFIYNKVGQSFNVLENHKILSTYENSLVAKFTIFRFVNNFIQFFITSFLSSYFVQLKLCQVNLSIPNDCFQILSNQMTTIFLTNIISLQIPKLVAPIIKAFIQNLLTKKDEKLVEHPFNLIDEEIERQLKLEPYQTNEEVDGSVNDYMELAIQFSYLSLFGLAFPACYILAFIQNVLKLQVDKYNFFNYSRRPFPQGASSIGNWLIIFEMISFLAIFTNAGLIVFTSEIIKENQVQVFTTLLIVFLTLKYIIRFLVPDEPAEALVLNRRHEFVVEKAVKGFQTESNARYLMQKINRQIGGPKETISSEEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XJW0</accession>
    <name>A0A8S1XJW0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1280006</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000128">
        <property type="protein sequence ID" value="CAD8201473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XJW0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8201473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="173" mass="20833" checksum="635F82F74B19BD4B" modified="2022-10-12" version="1">MLIILQEKSEFNITFQSIQTFIKQIIIMVYSKYQQQIINDQCNNILINHFIFGYLTIKFIWIKYLIQVTFAIIHIWNLFISACLQITQLNLVKYLDLHPYKTMVYITPYFNTGISTQISKQFGFQSNQYAQILTSFFQLKPEIYIKLYTYTSNFYLIYNSIKKFKTYSQRFCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XK66</accession>
    <name>A0A8S1XK66_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sfi1 spindle body domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1280074</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000128">
        <property type="protein sequence ID" value="CAD8201556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="300742at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8201556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1631" mass="199435" checksum="47058C894CB0585B" modified="2022-10-12" version="1">MEPVMQEIMAEVNGRYNLSNILDVLDKLRRAKRIDIDMYDKVYKQIITESRNDGDKWYQRYIPLKPKQLKELNSLNFSFDVKTSCDENNPNQTNRIQSSKLDKVLKFTSPTTFASPPTFETRGSKLTDFQSDYQKELTNQILYQISDGKRRSAQSDYIKSDKQHKTNRTISSFNINSLQQLISVLQRKIMQHYRLAFNKIIYLYNEPVKLLQDVEIQAEPSKVAKFSLKDSFQIEKEIQQFQENQILKILFHHFDNLKQYWLKKKRIKNSRNQVLLMRKHNYLWRTFNKLRKYSIKHKQNRLKKIQADHFYSLNQLRIYFEKMINFHLKQQTKNSKQKISSGLIQLRNKEILRTCFTKFIQYYQYRRNKHQNNLLAYGLYQQKLQKQYFTLWNNLVMNQQKRETNQIIMKKCLKKWKQYLQLKDFNRKRKQTADNLLIWQNKRKILKILKGYHIIIQNNNNYNKQLYDVYLFQLLRKGFKGFAINIQIKEKKKEQYQQIGRFLQILYISKPFNILQQYTKEQKMKKELHSKARAIYRVNKLRRLTNFWLKWAQEKKFHRKQDIYNIPQIKQFQIYLYFNKYRLAFQRSLWLKNLVRIMRSKYQKDFIKELKLNNIRFKQENKYPLKLKQKVISGLRINLINHLKKQQFYKQLLSLHQHNILKHIFDYMKVRGNNYRQKLINKRSVQRSLFGKYVAGPFYFWLNYSQKHSYKRNIRNNFDKLHIIKVYQKVFKTLNDYKWKKKIQNVQDSKNIPIIIQKQKLRFFKFWNAKRLSKGRIESFCISFMNLQQRIFFKHMQLIKVRDFQKKKYMAQGRIFYLMKFAFNQLNKYKIKKLKYRNNTEIIKKRQLYHLKQNSFKHMRKLFTFEQNVAYLTAITVLQIKKNLLQKYFKGFKELIVNKKRPRDQIMKMNKLRKCFAGWKVFTSKQQGTKLIVQAIYDIQKDQKSKFFRKLCSNNRIKNANMYYNQNLIQKVLNYWFDSTQSSQKTIYLTTILNKLIFKQLKKQFQKLLLKNKSIQVYENSINLIKRKMLKIWKLKLQKLKKFQRFLFQIINNRKENMIFSFQAIKDNSKEIEIKNQQSFQLLFQHFQKWKKIYQKSNSLKRIIFSIQIALKKTKICVFNRMKKINQDQKIVIQCSKLQKKRIFHTWLKKIHQIEGFNKLQIIFKKKEMEQFNNQAKKLFGINQQFTLNSIFSRKTLSKIFLRWNYLSKMNQKLILFESKLENIFKNKIYYQHCQSVLFYHLQSYQIKQLKGEFALKTFNKLVQHKQNAVFGQLQQDSIYYNYLDQKNIRKQIICFTLWAKLTNESVAYKFISKSLGEIFKRNMYYNLKIIKQFGDKNQIILKKIDDKKRKKVLLYWFLQCQKRIAAKSIYNILDTNYDSKQNKLLYVSKFMQILSQNQKKTEKGNKYYDVLNSLFKNKMKNALSKVLKFSKQQYLSKCKKRKLKKHYFKLWRINKAAITFDKILGMSYDRNVSYYFNKIKTQNQNQKAELHYQNKLKYQLIQFLQQVKDTTDKKMEFLSSKQNNNIVNVCFQTWIIELLKRNNQKKQFKKIFKGFYLYHYRTIGAEFIKQLKLEKKLQIKGEQESQQSLIYSVFKGWKMLVDERKMLKKYLQQCSVEEKRGKSIPKNMPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XKV0</accession>
    <name>A0A8S1XKV0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1280043</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000128">
        <property type="protein sequence ID" value="CAD8201509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XKV0"/>
    <dbReference type="OrthoDB" id="292598at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34894:SF5">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34894">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE RSMI, CONSERVED SITE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8201509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1088" mass="130938" checksum="C6F90D0EA9C083E4" modified="2022-10-12" version="1">MRSNLNIDISRSSSSHFLNDIELKKLFPFTQQLHNRYLQQNRLQNIKDDLTNIQQQTITYQIKDLLIKDKTRSRNNIDDIIYQQTTPRRLRSKERTISVNKKSTQTINYHDAQNVNLHNKSIRRDIVQLAQWLELMIQQVQQTQGNLSFQEYYNKIEVIFSGSMLELQRQLQQKGFEFGQCLKKIWDSFLINVQYVIEGLNGKANQIEQQSLDKINKLHEWYQEAIQKQLYQIQQQKEELLQIQQINIQMNKENQYLRKKDVRREKQLRELIYDKDHYLLEIIELKSIQMIHSIDQEQSQQKIYDYFKKQCENHMKTMEIQYKNLQVQDFYFDHTLDLIIKDKGCQVVDLIKINDQYTSTSDNQLINVATQTHKQKRQIDEEIQVKVNTTDQETQVSLYQIKRDKLIRNPYEQIILKNAYLDLDDIIDKFQQFYFPYQELLSNDPYYLTLINNEMNRQEKQINQIMDTLNIRNEIALNDFYKFKEYLKQINKETLQNYMQIWKIVLNQQSSVIDSIIEKQELQVELDEIKSIFTNNMLIIKQFQNKYEEDKKISEFYQQSLIRTIRYAPQFIINQIQQQANLYKVLDGVEFKEMRKKTTLVDSRISVSPQREDSSQLYSKQSPIRASQVSFQSQLQINSMKQITTNNKSLKAQKSFEIEVDMPKGLRDIDEESYKSESEDDNIQEKEDNAFSLLRFDFQTQKPQLTLEKKLRINTCPLKSAQHSTSILKQILKKFNKSNMNGLYTIGTLLQHYTELVQRQLPNNTNTPLHVHLYEHITFRFGQSQLSDCNRYKRMIKSFLYYEHRHSTCKIIVRFLKADLDVQDFNTYLQILEICKEYPSLTFNNFIQSLQDWLNQNHYTEEEMEIIKNELYQSDLAYLRNNPSPTEFDLIMDQFLTIFAKFKNRTSHKYKYLFESIDTSNKGNINFQQWNFIYETLIQVNYSFSLKLFYSEADYHDNQKLMSKQRFTLVSDELQIFQQDQQERLLDHQSFEDILLELKEKWSNNKIQMKICFIKSNRYDKFIKSLFQYVDRCLLNKQNGESCCYTYKLLQKTYKVYYLDQQVQSMLYSPLQAINLKFRQIEEKILSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XLC5</accession>
    <name>A0A8S1XLC5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1280059</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Asterix family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000128">
        <property type="protein sequence ID" value="CAD8201528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XLC5"/>
    <dbReference type="OrthoDB" id="284718at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045048">
        <property type="term" value="P:protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005351">
        <property type="entry name" value="ASTER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13193">
        <property type="entry name" value="CGI-140"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13193:SF0">
        <property type="entry name" value="PAT COMPLEX SUBUNIT ASTERIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03669">
        <property type="entry name" value="ASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8201528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="101" mass="11949" checksum="C46F48BF09D524A7" modified="2022-10-12" version="1">MKYTYDQVKGVVQEEQGGDMQQIFSFLLAFAGTWMKQKVFIWMAFFFLLSSCATMKTRSANYMQIILNFGIIVMGFLSLYVWQPKHEMLLQQQREQTLQQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XLF1</accession>
    <name>A0A8S1XLF1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1280075</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000128">
        <property type="protein sequence ID" value="CAD8201558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XLF1"/>
    <dbReference type="OrthoDB" id="300027at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="122"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="154"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="202"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8201558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="261" mass="30307" checksum="BF6672B82B143E58" modified="2022-10-12" version="1">MNEEIDHHLEMQETNAMLLQQFEKDTELQDQGTPNKYSDHSDPPSPTKKSKKKERIVEKLLENRSKFNDRTALFNLINSLQLVITLYNLSSIINIEGQTNLKFYDTTQIYSFYVWNKQGFTVYYLIIILIMVHILKLLTCLVGYFCVVQKSEQLLTIFMMLTFTCVITRGIITILLLIDYNQIELTQSYIYGDSDKVAQSQAVQFTIVLIVFVAVEIIMGLQSLLLAGQAKKKYKSMRINEKKIAAHYSIAYQIMLRQFVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XLN6</accession>
    <name>A0A8S1XLN6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vacuole membrane protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1280095</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000128">
        <property type="protein sequence ID" value="CAD8201598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XLN6"/>
    <dbReference type="OrthoDB" id="2016540at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="73"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="224"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="317"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="345"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="347"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8201598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="373" mass="42613" checksum="D2A92EC4DB6A3784" modified="2022-10-12" version="1">MVEAPTLFSNPIKTLYLFTIVLFNYAKDAVKYLSKFWHIIGFLALVVIAPRFVEGEHSELVRQGDEIAYFMLYWLVLGIMSSVGLGTGLHTFVLYLGPHIAKTTIQAYECNALPLFVPSKYATQYVECPKEATGITIFDIIKQVYFETVLFGIGTAIGELPPYFVARAAALANKKLEELEEVLEKKDENSFMQRMKLLIYNKLQKNAFLTVMLCASIPNPLFDLAGITCGHFLISFWTFFGATVLGKAFIKMHIQLPMTVFVFSQEPVKALLNLIQKYIPFIYQFLSDLLDKQRKQLRDPSLFKEGTKPLLAQLWDYFIILMIGFFMISIVNSLVAQYVTEQEQEKKKSKQHHHHSNKHHTGDHKTSKKAKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XVJ6</accession>
    <name>A0A8S1XVJ6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1400078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000140">
        <property type="protein sequence ID" value="CAD8205290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XVJ6"/>
    <dbReference type="OrthoDB" id="308390at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="289"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="319"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8205290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="359" mass="42228" checksum="4B16240407846CD7" modified="2022-10-12" version="1">MKSFRSEEPVSTTSKNKGFTFETGNQSTTRKQHKIPFQMNSLNTEECDNDTYRSQEAFKKIKNLEFQNDLFMMEMQHVQRRKEAEQNRLKFQLSSIKSDIDLMKSQLQSPVAKVKSKQKRSQSIDSELSSDGSSSSSSDNSYVKCPIKFKHFEPIQKEQIKIDSYETIENRFKKLFQISDVNEKLNWMVKQYFNSFHSIQLDLQNNLLPLLLENTLKLLIEIIEKLNSIKLDTLQFKSYDPFQNGDKNNKRYSSDLDIETINQEYQSQVNHTLTDYCQKFNEQQSQKNQFSTPQYKQQQQQDQQQSARQLQNMMKSKPDSSCQTSNNQTLDKKVSISERSQKKTTQKCQLMPSLKNRQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XWW9</accession>
    <name>A0A8S1XWW9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1410019</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000141">
        <property type="protein sequence ID" value="CAD8205555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XWW9"/>
    <dbReference type="OrthoDB" id="292704at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="234"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="287"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="331"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="363"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="3">
        <location>
            <begin position="477"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="764"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="819"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="772"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="826"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="838"/>
            <end position="852"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8205555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="935" mass="110887" checksum="DF5520AEBF3E0701" modified="2022-10-12" version="1">MNSELGSITERAPLKTQPTLPKQQLRPAFLKTVNLKTIQTNRMNSFQNKIWRDRALLVLMQVLRFISLITRSPFASKFSLLDRNMFRIIGDKAADFNYYLLNDYFKYMKPQQSSRVKYFLLQHLYKICYMKGFMDYLTTIKLTFEPESILLLFWNILMLVTINLNVLYITVKFSFDFDTYPPQDYEFCEIYLFKVPYFCFIIDTIIKLNTCYYEAGYLVRDRNKIWYNFYKNNFVINLFILVPTSIYFIAFQNSTLYLFMLLKAFSIPTITESIIDRVELTTNYWIIYDLIRLIYVILYESHICCCGLFYVGLLNKDSSWLIQNDLIYETWIIKYLNTFYWSIITMTTIGYGDIAPQNMIEKTFLIFVAIFSCCTFGYSINCIGQTIGQLQSKNHQIRVDMNDLKQYLRIRGYNNKLQIKILRFFEYLWKDQKNENQLDINKFNQQLPSHLHNVRQIILQEMMIDLNMKSISKIPFFKENFNEDFISALASKFIEEKLVPFNTIFSKNDPSNYLYILCDGEIEYFVEIPEGSATILSIQTISGQDEIFGQQEFLLDQNYEINCRSTKSSRILKISKLDFNIIAKKFGYEKYCQLKDLVKFSGRFDEFHLHCVGCNKSTHMLYQCPMLTGFPNKTKIIIQYRKNQVQERKFHNRNNLKRRLSSLIFEAQISDTVLYYLLQDPKLSQQISSQYQIQTFKQQQLQKELQNNNNNLQKQVNDEIRQTKRKPSVLRIKGRKQSSYIKRLPDFLNCQYINFKSMPEDKIKTGIDDQSKNVSAVHSEAQSSEINHNMKLKNQEELNTFEQNRISTDVAKSLPFKLINQTSSDQDQRKSDADSIKENSSLSQSSSKSPTKQTKGVSPGIDQITQCYMGTYKDIFEKFDKFQDYQNYMTHMNSFKILEQLNHFKETQNFTDFMNVNFVRKRRSKRNKLIVKIDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y3J0</accession>
    <name>A0A8S1Y3J0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1450063</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000145">
        <property type="protein sequence ID" value="CAD8206802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y3J0"/>
    <dbReference type="OrthoDB" id="342264at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17744">
        <property type="entry name" value="BRCT_MDC1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051579">
        <property type="entry name" value="DDR_Transcriptional_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23196">
        <property type="entry name" value="PAX TRANSCRIPTION ACTIVATION DOMAIN INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23196:SF1">
        <property type="entry name" value="PAX-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16770">
        <property type="entry name" value="RTT107_BRCT_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="5">
        <location>
            <begin position="518"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="371"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="429"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="371"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8206802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="663" mass="77277" checksum="E42621F39139C149" modified="2022-10-12" version="1">MIQVLDQNDKEIYKYRLTQGENKFGSDSKLCKFLIRNALINNDVYFTHFSDTSNVYKDSTLKPYSKVEIPKNFLIEIFPTKNYYFQTYKFKLLDLSESQQQNKSYQYGTTQNLEEFTGTMPLEDIFQIPQQSPKLSTTQSFTTTQVLNTQDPCQSTLIIEEADQSEIFTLNELIVQYSNMRLMNQSTSNVKQEVSTQTDQIEKEPPPIQKKGTLFSDLFKIKKQQQTTIDVIPKVQAPQQQIIDQTVIDSQLLGATINDDEQQKFFEQRNEIKGTIIDEELEKAFSSQFNNNSNNNEEQKSQISQFKRMNSVQSEQNVYTTPKMDINSNIELNDIFGSMPELPNQNIVQPQVIKKFSSLKSNLFSIKSDEIQEEKNQSQQDDTPKKFKQQENKGTENKKKKDKKKKEIVKKVGMLKKITDEISNNSISELEEEKIQPKQQQQQLPRHSNSMELPYTKILEEKASNIVPKSRRGLRKKRLVNNDRQFRTIAFSGFQEDKIPSEKDLMKLDLELIENQFEKFDLLIINTPFKRTFKFLAALMHGAEIVTADWLKDSLMQYEQQNHQNYVPESEEFQKQFGLSIKQIIQCRDDYFESNDNIIQIFEGKYYVNEDVIPSRAEIEYLIKLGGGHVVKKATKNVYIISESKKPNTYASDFILDACMFQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y5Y9</accession>
    <name>A0A8S1Y5Y9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Insulin-like growth factor binding protein, N-terminal</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1560047</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000156">
        <property type="protein sequence ID" value="CAD8209806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y5Y9"/>
    <dbReference type="OrthoDB" id="28293at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1091"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1133"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1162"/>
            <end position="1182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8209806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1200" mass="138464" checksum="6FAA7898C207F627" modified="2022-10-12" version="1">MAQMSRQNLFWRSCKLCIYSIFIDLIFLNQFCSEVLAQAEISRSFFTPFSTDDDWKVYLAENSNHITDCVTSQIFGGRLIFSQQTVITKTFILPPHYQIQFEFKFWRLNPWTAGYYYVYLNGEQAHFYNPYNATGEQICGSGTLGQIDQISKQIDHMLNSAIIIIVSKQTSAYWGISDFKLSLLKCPNLCDYCDSVGICQKWYRVLSYFTTLSFTNGQGWLKDNAIYDSVQDCGFQYYGNFLSSQVASINLNLNDPHTMLKFSFIFICVEITGSVTLEVIGDVQLYYYAPPLTSASTLNYFCGSNLKMTRIDIIGFSSSSSTMTISILIHNVATTSVNPVSFGIRDFEVFTYQENRIIIDESLIHKDDYLLIFEGIFSQQYNCVEGCSNCIRDICVECFSGWNFDTYSQQCIPICGDQQIIYQEECDDGNQQANDGCYQCKFSCPLNCISCEYGLCIFCNNNYQLIDNLCHFSCQFEDQNSLIKYDIQMKEGYYCQMSNFQKNTYLHHVIINNNLILEAVDIQCQIQNYGIFAYQYNRCEQRESQNCIISILNECQICMNKFERTLNGQCIPICNNEIYQEDVLIDNSIENYFVCSKCQLECLECHNSFCFQCLQGWNVVDFKCVYECGDGKIALGSYEQCDDQNQDSGDGCYECKFECSYNCIFCYGHLECAICKEYFEIKDNNCIPICGDGIVIDGLEICDDGNDIKYDGCYDCQYSCRENCQICDHQNCLDICDQGYYYLNNECISICGDLIIANNEQCDDANDDPFDGCDSCFFSCPLNCNNCYQGYCFNCNLGFIMNKNNCQDTCGNGYKSDIEECDDGNHLSLDGCSDICIIEIYWTCFEEDFNKSLCFQIIPPHFNLILLNQTYNIQYVQLQFTNQIKLLDSSQNLTQNFKAYLVDIDPSHYIISNVLIIEPNNLSVHNIIYQLKIQILEQLITEIFLQVQLNTVLVDQNGFQVDNDICKIRIKNPIVLTDEQKKISHKICSYNMTILIFLGLSSLIILMSGNPAECFEILDTIQYQANLKFININFPENLMIYFESSEVVTILPILQKFWILDIFQDVIGSQQLQAFGKFQNYNLNSDLITNLYGLFAQILLATVLLILSYCYLRFIFRTWFTKIRVFIYDNQSIIVSYHFAFFIHTLNQFSLGLHKILSQQGIVYIIQVNCWDLIFKTLLYLFSEKEMNPRNIAQTITAQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y732</accession>
    <name>A0A8S1Y732_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alpha-carbonic anhydrase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1550071</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000155">
        <property type="protein sequence ID" value="CAD8209609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y732"/>
    <dbReference type="OrthoDB" id="5986706at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF276">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_53, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035878007" description="Alpha-carbonic anhydrase domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8209609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="272" mass="31460" checksum="639AA2BCF02AFE64" modified="2022-10-12" version="1">MILSTIFILFYINQALAICSDDIPYLTTRILRPTQSLCDNTMMFNLSFVDYPLTIEIAKQLQTIYTIAPISMLSASDMHGNLNAYTATSFEFRSPSQHQIEGTQYDLEMLIHHQLQEGLVSDSQIAIVSILFKIDKKQSQPFFDDYYFKILKHQNTTTLTINFHNSLGTQIPQDSTFYTYMGTFNSHLLDCKQLVQWYVLDTPLPISSQQVDQFNYFFNGNQTKRENISQEKTTEVIDLLKLKGKYCESHLARDFGLFLGYCAMIFIIYKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YBL5</accession>
    <name>A0A8S1YBL5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1630099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000163">
        <property type="protein sequence ID" value="CAD8211465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YBL5"/>
    <dbReference type="OrthoDB" id="6252103at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="300"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8211465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="510" mass="60377" checksum="4F9EDF6B67320FD3" modified="2022-10-12" version="1">MNCTRHIQFPITSICIAPHQCQCQRKLCIDCQYEHGVDIKYTIPTHRFQDLVIKQLQDSKIDQTFEFTEQRKNFLMILSQTESMLKNTWQHLQESIKQIYDTIEMQDKSYLKIMNKDFNPTDFSNTDLEKLVQIVQGKIQNEWKVQKDFYLQKLLKVKDWWEKEIKAVNEKLMKEMDQNLSLNFNLKQQQNEQKSQSQVNLKPFSYEIIQANSIKQQEYCMAVAINKDCSIVAASCYQLIKIYDFNQGMMKQNQVLNQHQSDVATLNFMKQSNQLISGDNGSILIWTYNNDNSWICSQNIKGHSDWIRCIILDNNEDLFISSSDDKTIKFWVKKNEWICQQTIQDHSSCVQQLSLNEQQNQVISCGEDSLILIIEQSQPNKNWIVKQKIQVDCCGLRLCFINNNLFTFQPAYGNMMYVYEMNSVNITINQGNEVGWLFPQQFIKKRQLLVNKHDKYVNLIRFTENSQFKVEQSIQFEKNHIFGQLSDDGEYLITWDETSKEIQIRRFKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YBP8</accession>
    <name>A0A8S1YBP8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1650105</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000165">
        <property type="protein sequence ID" value="CAD8211906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YBP8"/>
    <dbReference type="OrthoDB" id="66726at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="207"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="257"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8211906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="304" mass="35780" checksum="9BFDF403DBD79FD3" modified="2022-10-12" version="1">MKSKTIFYVGAIVGIYYFTKSAVKQLLKFKSLLGERSSINYLFKKIEKQNQEQNGLQFFTQMYIAGSPQRIVSLCESNPNLKTFFLISQIKLRQGQDSFFSSNIVERSPIFSLKNAYDLEVQINALNQFSFNLEKARIMFQEEMSVYRALLFNLNLMKREIIAGLPYNKQYLIFGDFIHNPKTKVLRCYRIRKLTEIKDYPYLRFQFYFSNIISILLKIGLVASLSYFLYNSAKKILYQDINIKDVLVSQYHQTLSCFICKERRANIIYEPCFHLSCCMECSQNKVNCPVCQKNILRQIKVFNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YDB7</accession>
    <name>A0A8S1YDB7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1640071</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000164">
        <property type="protein sequence ID" value="CAD8211611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YDB7"/>
    <dbReference type="OrthoDB" id="10001977at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF122">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN SKYWALKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="2">
        <location>
            <begin position="129"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="15"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8211611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="303" mass="35281" checksum="5B82C7C332602C73" modified="2022-10-12" version="1">MQILIIAEDNSQLLLVNQQQLLKLQELNIQQQEQLKQLNQEIDQLKKNELQLQNELQQNKQLNDTLNTKLKEQQQQYQQLQDTLNNNIKELKTKDQELQNQLLQHQDLLKELKCVYPYISIKWTIGKNTILKDDYFSKILKTIEEKTKKKVKNQYFIYSGAQNGLNGQSFWKSVDKLSNLLMIFKSKSGYIFGAFSPCTWMSNCNGYIQDDTLSSFIFSQTHDQIYPIKEGYKQHAIYSGQQYGPIFGSGHDIYISTDFQGSSSNLGNSYQCDQFQINNNRTHLFGQSTPNIAECEILMLTFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YHX6</accession>
    <name>A0A8S1YHX6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1660083</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000166">
        <property type="protein sequence ID" value="CAD8212097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YHX6"/>
    <dbReference type="OrthoDB" id="10249433at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052920">
        <property type="entry name" value="DNA-binding_regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43358">
        <property type="entry name" value="ALPHA/BETA-HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43358:SF4">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="51"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8212097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="321" mass="36948" checksum="6B3F9D138E1E2909" modified="2022-10-12" version="1">MFDNYCRQIMQPKRAIFNRSDLECGYPRHDFKLQGLQCSLYKSNKTTQICVLYLHGYNGSRLEAVPYATAILQSQMDLCTFDFQAAGQSDGDFVTFGLNEQLNVVFLVDYLQDKYQNIILWGRSMGATTALMYALKHQNIHCLILDSPFIALEEVILNLIKDKLGTPDLINKGLFEIIKRQILQLYKFSISSVKLPQCLNINCPMLLLGSKFDSLIPYHHFTKILDSYQGQKQIIHLYTNHNELRSKDIINTIIGFIQSTILLIKNNPKNRFIVDIQSKQYIATGIKIKQKIMRNQSNVNYNQNSAILQKHKALLQLSNID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YPL2</accession>
    <name>A0A8S1YPL2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T2460004</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000246">
        <property type="protein sequence ID" value="CAD8214747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YPL2"/>
    <dbReference type="OrthoDB" id="2443807at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8214747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="250" mass="30142" checksum="6EE9646FBCB90A0B" modified="2022-10-12" version="1">MIIDITKIATQCRQSHQKECLKSKGKNNQNEEVTQYQILRGESVKYLLEIRKRNVLKEATKQNVKSIYVLQNTQEKDKNEISSLMNLYQFHDKIREENELLFNNKQKVLLIQGLAEACKSKRAKKIEKFIWKLHDRGINHEDDYGFEDLQFIEQKDILQKKESQFLEKFYINQTHIKRNDIFTSNNYAQWLAPEDKNQFKEIQLLNFDQVQKQEYFKKLSIQSITMLIFDITEWQVYPQNQNRWISKIQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RZA8</accession>
    <name>A0A8S1RZA8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0020078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8132802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RZA8"/>
    <dbReference type="OrthoDB" id="288795at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8132802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="22040" checksum="94B0E318C23CC7C4" modified="2022-10-12" version="1">MCIPFQSIPPPTQGTKKAVQMLQILGLSQFVVGLIKLFVLQQMTGFYEFFTIFILYMAWAQLNYCNCVIAVFYFLQNLLMIFIFFGMNVELSISFFQPTKIGITYFIFFFYYIGACFVAFLAYREFKILQYDQMGGLIQTDQQSDQTQNNNQQQQYQQQQYYNQTVQPQQQAQRGQFQAFQGRGVQIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S637</accession>
    <name>A0A8S1S637_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0040396</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S637"/>
    <dbReference type="OrthoDB" id="307815at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8135736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="152" mass="17899" checksum="4801E80EC0024524" modified="2022-10-12" version="1">MNKVNTKSKQQVQNDFDSYQSDNSYQSDGDNQPIIMNFLEDIKGHLDGDISFETNEHDIYDPDFSQNISHRIKEHQDTAFQKQKFKSNTQSIKSCNSINIICSLEIFQQQLELKHTQSLPDQQNLQTISDSKKQQLNFLWEKIRQKNKDKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S6A0</accession>
    <name>A0A8S1S6A0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0030340</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S6A0"/>
    <dbReference type="OrthoDB" id="306128at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8134504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="838" mass="99232" checksum="514372347361D5B4" modified="2022-10-12" version="1">MYQQNWQGDRYQDEIFLDDVEVYQKDEEPSIFDIVYREVGCILLKYTNLNLITCPNTLHYITLQFNNDEPFSFLFQPNYILVEQLAVNPALKDIAEQLQIEDLINHNQNDPEYFQDYYQHLIEEIFKSHLNQAQEIFLRQIELLQEQRIKPQVDQELQSIFQDKQFQFPLKLDFQNSELPENQIKYKLFESLQLLQSNYLNTQSKGNKSIKLESSETVQQLSGQKTQQENVNITPFVQPLVISSLNKEKIKMIINWLIQEKIAIPEIILCAELKNRGNGMNLTFNPNQQMLNKCAFYYDLFCEAQQNQSETKNIKEENTEEVKTCQEFNIGKQKTKITEYHLLIRMLDLVVQQQESLKEKIIQLVKYSQLKNQIKRLSRVQESDAHYKIVKQFIQKLETILCIYDEYENDKQNQFKNQVDKKSQINSDNLSENEDENKPIKCQKKYYRLLKLVVKLILIESLEKEIPIPEILTCLWFKKHKEKIVEGQIINQNSVQILPSKQRLIPSLQLENRLKLKQEQVQLQTLLDLPDTATKELDDFENFISQFTYDIKYGRDVILIEKILIVVNNKKDSIQDQLIQVMNDYLKIEKAKDQLREKLILLPKLRTNKTQINDEPILENIIKIIDNLQLYISSIQKSKPYIITKQINNRTKKQLQQSSQNQQIQQFQKLDKEELKVFILSGLITLIDNEVSLPELISYLGIKNKTQKFSLQINKNYKLQEYITIQMQLNQVEITDQIESKLLIKKIKIQEGENIPIIHNKEQVSEPQLIIRALRLLSNCDMTKQLSDIWQKPSVISLKNQVYKNCLKKNKNDTTIGQIKNLFYKIDNKYEIFNNNQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S6L0</accession>
    <name>A0A8S1S6L0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Poly(A) RNA polymerase mitochondrial-like central palm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0040067</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S6L0"/>
    <dbReference type="OrthoDB" id="299395at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271">
        <property type="entry name" value="POLY A POLYMERASE CID PAP -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271:SF40">
        <property type="entry name" value="POLY(A) RNA POLYMERASE GLD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="1">
        <location>
            <begin position="35"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8135127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="335" mass="39023" checksum="FB53BA9B9ECC377D" modified="2022-10-12" version="1">MEYNYSQDFEDQLLNLINQEIPNILYLSLDALATALKMTKLKGNLYPFGSFCNGFESEIKDLDCVFLTPCDDKPTSLLRQVQAGIRAYNEQNLIPSLKINAFISHAKVPIIKLVDTTNNVEIDLSVNNINGIANSKLLYEYSQLHPKIKQMGLLLKLWGKRNRLIKTGSLSSYGMIIFMIHFLQEKYKVPYLSDIKLTAQHLKNLEQLENNPYFRIGLKVDLPELTQASLQQLLYEFFSYYQPQGEFQQKNICISIHRIPIQNVHHNFTLKIQDPINLRQDPSKRFELQCLEFQNAFLKAKKLIQQHDIHAFDYNDIAKNKVKKNNKLHQQFQQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S9I7</accession>
    <name>A0A8S1S9I7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0050415</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S9I7"/>
    <dbReference type="OrthoDB" id="300452at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8136926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="191" mass="22423" checksum="3ADF491196D09F93" modified="2022-10-12" version="1">MIHLPTIDLISIPKSNENEKNEASFDFRRFKLKKITKMNHSLQIKLNIVNKDSNAKNQVAFGSSQERRKPSGKKFPPLQYDLNFKSVDRHQASPKLRTIRSTRIKSEVSQQYCERFPYSFLKTETIEQTIQPTKLNCLQKLIKKVRSPKIELQSSIMLKQDEFIPMSSKELKESLISLMHKYGIRQNEIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S9X9</accession>
    <name>A0A8S1S9X9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0050485</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8137066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S9X9"/>
    <dbReference type="OrthoDB" id="297041at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="624"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="132"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="289"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="624"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8137066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="655" mass="76800" checksum="0726DC5814D48429" modified="2022-10-12" version="1">MDNKSKGSSKSRKELGDELSLKGVSLFKLDVNYDHLKQFLEGVQADLDNHTVEIEGIKKLLGPSEIILLVQEQFQLLSDSLHFSDKDIEKELSTQLSTYITQIQGKQKYGKGALEKIITEMRGKLFSVISMIQHLYREKKRQDQRLTKLENDMLTKFDTKECKEKMRKQKNQLEQQIEDLQKNNRHELDTVEKKLSTDIQKFEEAVRDVERKTLWKIHDCQDLLSKRTNEEFVNEAIRTSEDKLLKEIQQMQNNDQLIKFQQDIAYIRSQIKGIDDTVGEKQSKLKIQLNEMNDLLKSKINLIDKLVEQNKQQGDKIIDLNSRLATAINKLDVSNQLDQHRQKIKQLEDGIQRLNDEAKERVEALKDMNLNAENVAYLLSKIDPHKICTLEGDIKKLEEMSIRNNVHWETLDAEFKSRYDSFQFFSQLYQQQQQQQQQSTPEESKYVTKDEINRMFEQFQQQGNLEDDKIRKIQKDLQDIGHKVESANEIERRVNRIFRDVDINGLMKQVKTKANEDDMKRELFNLETRLGQAMETINYLRREIDALQVQLKKSPMNFQQSASPPTDQIIGINTKKLYPINCLSCSTTNQQRVKGTDGKYYQADMKRQTIDQQQFYDLQQDESQQFNQQHYVQRPQSAVVNKQSNGAKRPISAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SC36</accession>
    <name>A0A8S1SC36_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">1-phosphatidylinositol 4-kinase</fullName>
            <ecNumber evidence="2">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0050128</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19877"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.67"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SC36"/>
    <dbReference type="OrthoDB" id="10264149at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00893">
        <property type="entry name" value="PI4Kc_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000016">
        <property type="entry name" value="PIK1p Phosphatidylinositol 4-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF22">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="5">
        <location>
            <begin position="526"/>
            <end position="798"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8136394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="814" mass="95490" checksum="2247782BD2DCAE78" modified="2022-10-12" version="1">MQQEEGANCFCFQKKNKGVLPKTFKKTNEILRKYKNNFLDISDSDILEVIIRLYVSEDKQKAISMLSRCRINPNFQSNNLREDLEYFIPQMVNFMVFHQQLSDENLKQFVLKASSLDFFFAHLVYFQMKSLSQIITPHETVTINVVKKFLEYFLFSMAQNYSGNLLIATHILQMNLELSNLKDDNSDSDENSPKKSQLQAVYQGTTKQQSMKVNEAIQNYGTADWIKYQNSHPYDNRVKNHKEIELQDYESIFNLEKNEKTLDTAFLSNIQFWNDIMRICDELSKVQTKKTEVLQACLQKMNKNLPASVYVPFVNNQVRNYAILKIVPKESRVFSTKMRSPFSLTLELYRPEIEGQSNHKFIEKKISRPITMSQYKLLQRTQSQLFNEDHSIIENRRTFSSTDAQKQIQNDFQQIKFFQMNPQVHHPVIQGTSQFYYQHPTDENEEEQIDQQKLKPEISSTYSLEENVQAFSIDEESSITQEKNGEGNWLTSIIGKNSFIQPNRGLVLTTQEHLEMKQAIFGENSLDQFERIKSQSIFSQLQTWNIIHLIIKTGDNLKQEQFALQLISQFDQIFKKEGLPLKLRYYEVLSMGPDCGIIEMIKNATTIDSLQKHLRKEYTQFKNFSDFFRSFFRHKIDQALENYVQSLVAYGLVCYFLQVKDRHNGNILLDKEGHLIHIDFGFFLSIAPGKGVEFEKNVPFKLLSDYIEVLGGVQGNLFKENFRKLFFQGFKACQKHQKEILLLAEMMYTGHGTTLPCFSKGEQTLKELQLRFNPKVQSSAELYVHVQELIDKSLDNWRARWYDKFQYFAQGIFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SC98</accession>
    <name>A0A8S1SC98_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0070254</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SC98"/>
    <dbReference type="OrthoDB" id="287576at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8138671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="139" mass="16360" checksum="278AEE811B42BD98" modified="2022-10-12" version="1">MGLCSSSQQKQPRPLIRLVKVEMDNKDVNYLQTLNLWRTIDKQIVLLKNNHQISSKLLNQTDQINTFLILNQGQNLKKNLQTFFKLFDDFQISFTQEIMQNESIWVNYSCIFKNLDQLCENFADIGFCSKVQTQSLTKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SCI1</accession>
    <name>A0A8S1SCI1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0060032</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SCI1"/>
    <dbReference type="OrthoDB" id="290593at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011:SF28">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="141"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="238"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8137320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="492" mass="58188" checksum="8C774BCD90AC0A4C" modified="2022-10-12" version="1">MLYENLKQVFCQKQKKKEYVEQISKIVGDLKFFKEKREIFDSILYYQMLKKLNLIETKQEEVLFHYGSNNVELFYIILQGKVAILCPKNRMISHKQILDEYHQIDIVGSPKSSNDSLKDIIIQQAYSNFLGMKNLKQFDQDAIIDEYNHNPNNFQVDKALDQLIKKRFPSLKIVNMCHQGDQFGEIALIGHVRRTATVLCVTDCLLLTLTNNDFQNILQQYHQQVRNEKIQLLRNYSLFRSLSDGKLKGMIEHIIVQKFSIFSVIYYENTIPEYIYFIKSGEVELLKVVNKNKQISISLLQVGCVFGHQDIGHQQFRQYRAISKSCQCELYLIPVNIVLQFDQQNENNEVEKEFHQSRLKRLLSDRISVHDRKKQNLSPQSIEERYKGDKDYESFLSYAFATKRVLSPKHKKLISEPFVPFFDINHSKQKKFRQHTSLSKNQSVEKQTIINLDHFNNSKNQLITTQVNYHRHLFSPRTTKRHLDTFMPFKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SCV6</accession>
    <name>A0A8S1SCV6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">EXPERA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPENT_87.1.T0060294</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SCV6"/>
    <dbReference type="OrthoDB" id="58557at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000247">
        <property type="term" value="F:C-8 sterol isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047750">
        <property type="term" value="F:cholestenol delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004769">
        <property type="term" value="F:steroid Delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007905">
        <property type="entry name" value="EBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033118">
        <property type="entry name" value="EXPERA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14207:SF0">
        <property type="entry name" value="3-BETA-HYDROXYSTEROID-DELTA(8),DELTA(7)-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14207">
        <property type="entry name" value="STEROL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05241">
        <property type="entry name" value="EBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51751">
        <property type="entry name" value="EXPERA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 13 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="7" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="10" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="4 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="EXPERA" evidence="15">
        <location>
            <begin position="24"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8137788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="188" mass="22180" checksum="30742125AECD9434" modified="2022-10-12" version="1">MILGSDNLILAGFIIASLFLIKGPNFQYYWFVFNGIWIHLYLDGTVGYWQKHQWLFQKYSELDLRYPLQEPTLMTISAIEFFIMGPLCVWIALRIKFQKNEILTNFLIGLTSAIQIMGTIFFVANEWSRNFKDVCQYGCFTYTFANIFYFWIFFVAANPVWIIVPLQQIITAYKNLKNLFEPKKSKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SE80</accession>
    <name>A0A8S1SE80_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0050419</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SE80"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="185"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8136934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="354" mass="42631" checksum="9615092473FB1729" modified="2022-10-12" version="1">MLIQFNNVYSCKLRQSFLDQLYDYEYNVVSVIYSLLFFNQNVTQLTVRKTNYQLCKELRQMRTPNDQQSFGLRQDNLDQMMFQQHNPLVTQKHQRAKSSLQNNYKQGTPNTDLEELEGDKLFDQFFKLNNSRSDSDFMRESIQRETKSIESDFLKFVENAIEYHKQSELHFRPLIKKNPQLDQKIKELNLSNRFLANELNRYKQKEHVLKNKVKLMQKEFQSEITQLIQKNEWLEELLVKQKIDNENSLLALKKSLEMIQGKCKCQKDVKSQVKQQFDLQATHSSSELSSNTEEEDFQYDFFKRRKSLSKVIYYILMFQNSDDHQFFQSKIKNQKDYSRDFFLRKKSAQDPYYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SFY0</accession>
    <name>A0A8S1SFY0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0060009</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10035969at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035934042" description="EGF-like domain-containing protein" evidence="7">
        <location>
            <begin position="19"/>
            <end position="2169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="729"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="827"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="933"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="988"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1014"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1052"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1076"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1522"/>
            <end position="1543"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="39"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="84"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="123"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="169"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="211"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="260"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="313"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="360"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2147"/>
            <end position="2169"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1473"/>
            <end position="1500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8137274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2169" mass="252548" checksum="7BD0B5F302E19859" modified="2022-10-12" version="1">MIFRLLFIFVALVQITYQACRKDGCWSCKRYQDCCISCGCGWSLKYQKTIDTFGCDNNKCDDYDDWKCKKCVDGYYLKDYTCFLCTYPCIECESRTKCKKCDSGYKLSNDQCIQSCTLPLCKQCDNIPTTCDVCVDNATYDSPNSKCLCNNSYYSDGKQCKQCISICGSCLNLYNYCATCKSGVSNMSPNPINGVCSCTTGYYWNSSSCEQCVAPCLTCENEANKCTSCNDESLMVNDENECICRIGYLSSPNNPYQCEICSNNCFTCADVSNKCTSCNENYELNEDTNTCYCPDGFYEINNKCNECDKLCSKCLSKTICSECIENPLVRFSNQQCICMDGYYFDVNQSLCLQCELTCKTCQTQKQMCTSCNSELNRTLSNNKCICKSDHFLNENQLCISCKSKEAIAIEFCKYKDCTDGIWSYGEECDDANNLSRDGCFNCLKEPQYYCINEIEKQSQCEKCQEYCQICEYDYFYKIQKCKQAIDGYYIENSIRINKCQDKCLHCESNASQCTKCRFLNISKSNINCQLCEFKQGYYSDYDNNSCYSKCGDAILADVEQCDDGNRINGDGCSDICQLEKSFDCINGVCIKTKNPIPQGQENQIYDLYTPKRQVLVTYDQELKLNQIFDINDEIQISLNQQANFNYSIKNNVNFEIDNLINFSFTIDFDVDRSISNASLIIKYLTPSLFRNAQNQTQEINLLEIKISDIVIISQSVQGLTTNTVNSSNYIIYSLLALLACGLILGGIDIYFNLIDTLQYLSYLYYINTIFPFNVNSFFENLNFAQFTIVQDFINLDQIFQYDLEYDLEFHSLTVPFKIKKQGMYSCFILNFTSIFSVFIISLVFYLVANLITIKYLSSKKQLLFQSDNKSSIRMYFSKLQVKLKHLAFQWALITIREFFYSDLLRIFMTTANEYSFTLALGLKGLDFNSTFGLINSTTTFAFLGIYICITFLSFKIVDQKRYAIEQAQYNIKFGSVFSGLQIAKYQKLYNPFLLIKKFLFMTFLIYLYEYPIIQVLQVTLLSLVTLFFLIFYSPIKDKLELIKQISCETSQFITLLLFTIFCINDLLKFLNADQKLIIGWVVVSNVLTALFTQLVINTIQQWKYLYIKYQFIRKIANSCSRRCQAKAPTQAPHDVFLMTNIQHHFKLIVTQTIFLADLIETTISLSVLFVLVMYWINSQISSNNETIIEIMKANITLTGIDLQQTISSIGFLFNITKLALNEKEISQQNSTLNTVIGTLTVKERSQIAQIEEITSIYIPNLPYYVIQLTSDSLSASNNTLNLYNFKSTPNCTNNPKFCQLLHNSYDDKGNFDLFLYMKNQKKDQVIFYTLFPFNIQLTQICGLLSNDSLYQCFILDLADYKQPEGILYTTLFTINNSVVSDVTDDCLTVEMKCSAITEFNNMDIDYQNTQNIVEFLESIDKADWMNQMRPLTRYYESSNPIVSLSMVDIKVYFYQSIHIGTYDINDEFYSEYKEDLINQTKEEQDKLNALQQNYDRWQLLATFNNQLYKNLVETKQEDYFDFFLFFQVFILFLLLTSILVNIITQNEFDQISITLNQVSMMMQMFFELDYDTLHKDLNAFKEYQKSSLTELNELLQIFDEFCWLIDFLNQLNKKVHDLEKLEQGLQIFQKRNSIECLELILMKLVSEHSRQKDYETAITYNKQLISILEQQKKDTQLIKSEEIMRINYKLFQSLNQLLDLLSKLLIRNKSRDRNAFNQAKTLFSNIMDFADTLEIKFTLQIKLFFKKCKLLFNLRKIKEAEQCIDQAQLILQQNYTLSKNASYQNKTFNQDKKLLQVVQQKIYFYRGLIYMSSGQLQPAIQQLMLALDYGNVYQTKLRVKTIKMLSTLLAKQDKFKSDSQILKQLQYFFQNQKRQFIFLIDSTQRMAENQLEVVETIKQIFQIMNDDDLIQVSTFDEYTHVLIESQSKISILETMGKNYMEDGFFDYLSGRQTQNQRKLYQGILESLNQQLPKKYTNYLIVMTFGENYAEQNDKLVELLEKLREGFWHFILCSTQEKKFMNQRNTFYRLTDEARDGTYIELFANFEEGLINMFDEDFDLGKQEDLAREVKEMEVKANTLQEKSQQDRYLEFSEMVVNSFKMMEKKFNEMESKFSKVLQYKNKENTTSNIRQKSVSVGKIYAPPKISAGKISLPKHQPPQLKKSILKTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SGZ7</accession>
    <name>A0A8S1SGZ7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0090164</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SGZ7"/>
    <dbReference type="OrthoDB" id="302782at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="53"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8140591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="292" mass="35446" checksum="18D33A39B94C4BC7" modified="2022-10-12" version="1">MLSARDQLSFSRKTIKHYSIASQKPIRYATQYYGDNELSSSIFNSDYELIRTKEKYKQKCEEIDMKKNQLHSNHLDLELRAEKLRQGENEIKYMAELLKKKIEQIKRQEQQIKENQITFVDKFKDKEQSIIEQQKLIQEKQDQLNIREQQLIIREQSLKEKECLVQQQINYYTQFNERLKINLEELSKKEQFYIQQISKMNSAIETLLYHENHIRDLEFKLTQQIQGLKDFDNQIRIQVQNCKQQEELIDQQEQGILKSQDLINRKLLWDQKNITKQHLQQISNYMSDQNYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJX1</accession>
    <name>A0A8S1SJX1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0080212</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJX1"/>
    <dbReference type="OrthoDB" id="286986at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8139637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="176" mass="20568" checksum="25300DA0ABD4B1A4" modified="2022-10-12" version="1">MKASKYQIENRDYNIITCVDFNRQQQSLPPLRKLGQSQDHPFPRKAKFELAQEQVQMQQTSSSKVNNSQQDIKQVQPGSMMNVPSVKFDFISFTQGKQQPFHDLKQELKILHKKKGGMGEIADLGGLCYPNFNREYQDKFKENQTIFRKQKGDYPAVTDPKFSYGPFMKPFKKFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SPY8</accession>
    <name>A0A8S1SPY8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0090133</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SPY8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8140554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="183" mass="21191" checksum="E89CA157293DF75E" modified="2022-10-12" version="1">MENLAVAQTYFPPQTTFVSIYDQQISSPFQKSLLVQDSKTKEDFILLGTVKSQNCYIKYFESNIIGLCLHLVTQSYLMQILFQSIEVFGHNLTQFPSDTILYPVTHYEQVDSVHYSLKCKAKTIQNYQIQIQINNLYVKFPLIEKVKPPYSTQRLVLATTQYCVRCIINIKVIPNILCSIIVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SRK6</accession>
    <name>A0A8S1SRK6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0090450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8141122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SRK6"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8141122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="140" mass="16782" checksum="B81EE12EAD11BE06" modified="2022-10-12" version="1">MENPSIALVMSSETRMKEICKLRNLILQKRYLKNGLMLFYQQFGQEISKQMRIFHCAAGISRSFDIVRTLFDFSNIYINQYREIQSIQIFIDNSLQDKMHKIKPQKVLRRIKQTRPYENPNKGFTKQLEKSNQEVMEQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SSX9</accession>
    <name>A0A8S1SSX9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0110140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SSX9"/>
    <dbReference type="OrthoDB" id="10322165at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="186"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8142439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="338" mass="39717" checksum="6753B83ECA95EC12" modified="2022-10-12" version="1">MFYKPKLISPNRTQSPVVVHKQSISQVNNPLLTQQQYQPQTYYASSKTTPIKNLEQKFELIKQNINITNQCQLSPERNIQAVNNDFENQILRNDIVFKDQKIVALQKALDLSNDDRIKLRQALEQKSNLCVNKEREIAKLLATIHQIEFKKSESQIVKDLQFQIETLKTFIQENALDKSNLKNEDTNQFKTKVANLQEQLAQQINQNQSLQQYMKEIMNQNKELSQKYTEKCIELQKLEMQLDQIKILQNELKGKAEEIEILKGELSNDNTTIQIKEQSTFDQQNEIQKFLCQIKQQYLEDNSKLDIDDQDNMNVYRHLYSFQSTDDLTKGELQKINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SUA9</accession>
    <name>A0A8S1SUA9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0100486</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8142144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SUA9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035877734" evidence="1">
        <location>
            <begin position="16"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8142144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="104" mass="11981" checksum="33972458B5ED96CC" modified="2022-10-12" version="1">MIVFILLICMNTSKSFKIQTLDPAYQVAYQSVKIEEEISQPNQCFNFGLWSRYNPLSSISHVGPVGIFATNFSIQLIQSMMRQFSCITIVQIMRKIKQRNISNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SY37</accession>
    <name>A0A8S1SY37_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0150377</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SY37"/>
    <dbReference type="OrthoDB" id="305544at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="47"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="383"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8146501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="479" mass="57816" checksum="9FAD990E49EF75AA" modified="2022-10-12" version="1">MYKPSRSLRQSQEKLYTHNKPSPQTRAVSFANTMEEPLRQINRELDRDLQRQKLKVYETELLEAKDQQSQICVELNEQRLRNKQQQQVIENLEFKLQIERKDSLIYQQQVQRLEEQFNYLQDNYNTQKQQIIVLQESIEQIQKKNNLEKEMRNEKEYLLEKEIKFQQNEIKRYQEEIQKQREHILQNNQQQNANEFIQILENKFELVQKEFIFYKKEKEQEIENNQQLIDSYQNQIQLLSQENKSLQDKNQIVSTTEEHQQQIKSLKIELESQIQIAKQLNQQIAILQQQLNQTNVICQNLKEEKQSAIKEKQAIETYYLDQLQKQDVQLSQMIKSSVQNEQKAQIQKLQIIIEQKDLMIRELSMQKDCNCNKQIKNEQKLIIRDLEKKNVTLVMEVERLNKILKQKLTELENQTSRSCEENHKKYLEEIDIWKNKFLSINKQYHISQEQLMVVKTELDGLKKAKQKENIDPILTNKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SY54</accession>
    <name>A0A8S1SY54_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0140051</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SY54"/>
    <dbReference type="OrthoDB" id="292972at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="5"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="358"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="5">
        <location>
            <begin position="388"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="6">
        <location>
            <begin position="650"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="708"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8145053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="776" mass="89405" checksum="1753B050F7089500" modified="2022-10-12" version="1">MLYIFGSLLISHLVTCVVLTFYQLNDSRLINTIMLSFQFLMLAIHFIQKIKQIQNEKIQLSVKVCIKISLLIIIFQRKQNLVQSFLAGSYMQYLLKKQSIVLKLIDALICMFYFIMWGETNENTITGTLLSLFMVLNICMKYYIQNKKEKESGGQISTSKLPSPGLIKNQTSPNYSRKASLHEYQSEPSQDLQINDFGCMDQYQKIFNMFPDGIIMVNMKKQVIFINKHLKKMMGGMNKKAAFYNLLQMKNVQQQINELERINSLFQEQQIILNEQEFDNKTYFKKSTFDESRFDDQSELKSSYSFQQFETVKKLLDHLIENPKEKNGVIKMLCQFPQNEKSLFQVSFKRFENKKSEFILLIVTDMTSMNLVTFLQRVITDKSNFLSTVVHEFRTPLQSIKLMVQQIQPMCSEEMVQKYLLPMLSQLDCFTNLIQDLLDVSVLNVGKFKLDIQQLNLHHLITETYNIMVVQAYAKNNDLVINLQGIPEKICSDPYKIRQILINLLGNAVKFTYNGIITISEITVSDTGLGITAANQNKLFTAFGKLDDPTNSNPQGIGLGLMISNVLARKLSYDQKGLSAVSEGEGKGSKFSFQVYDHSFNEDKQKYQQESVVRQQREYFQAPLNQIKQCDSFRMSGLELSQYSRCQCPEILIVDDQPFILQMLKIQLEQSGYRVNLAFSGFECIKKIEQFQHGPSCACKNYKIVILDIDLPNLNGIETCKRLISMGFNGYILGHSGYSGGQEEEECLKAGMVGYFVKPLDIAKLQQWLEQNRIML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T107</accession>
    <name>A0A8S1T107_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0140138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T107"/>
    <dbReference type="OrthoDB" id="310807at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039273">
        <property type="entry name" value="TEPSIN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21514">
        <property type="entry name" value="AP-4 COMPLEX ACCESSORY SUBUNIT TEPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21514:SF0">
        <property type="entry name" value="AP-4 COMPLEX ACCESSORY SUBUNIT TEPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8145229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="319" mass="37079" checksum="C8862568FAC27829" modified="2022-10-12" version="1">MSSILNKFRKQPQNDIPAGIKIENTLFGQQNGTYQPPLIPAVQLPLTTQPRQKGRPGGGWDDESEPQQQQQQQILSNKPTNNINLINDTPPKQNENKANIDVLLPPQSYNNNIQLSRVAEYKKGQLLTYEELEIGGFEFEFHLVEEATKLGGIKLKQSDQVLKEFGRACQTLQEQLIGSILLNKIYSEENWKVQIRCIYAIQFISQQYQNYREFFQINQNYLKIETDQQILQSAIEQTLLEINGLQQQKQKEFQFEFREPPKQQQNIVQKQPNLIEIANNEVQQKQPQQQKLDLKSLKIKQPQVQQQSQQQLQQQQGIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TCL9</accession>
    <name>A0A8S1TCL9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Zinc finger CHCC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0190220</name>
        <name evidence="3" type="ORF">PPENT_87.1.T0230144</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TCL9"/>
    <dbReference type="OrthoDB" id="307899at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.40:FF:000004">
        <property type="entry name" value="Lactobacillus shifted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019401">
        <property type="entry name" value="Znf_CHCC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13156:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13156">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 13 KD-A SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10276">
        <property type="entry name" value="zf-CHCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zinc finger CHCC-type" evidence="1">
        <location>
            <begin position="62"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8149658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8152619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="111" mass="12714" checksum="52BF9CB225B9CACE" modified="2022-10-12" version="1">MLQKCIQLAKSAQFSQMVKSYTSLAQHKWLDYDETHKRSLQTHKSRAEEFIHKIPVIEVDSDVVRCLGGTHINAGHPQVYIKLDTRTEGTPQTCKYCGLQYVKKGHGSHHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TCQ8</accession>
    <name>A0A8S1TCQ8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0190027</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TCQ8"/>
    <dbReference type="OrthoDB" id="301615at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="355"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8149274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="672" mass="80174" checksum="3F523AD979F80C8D" modified="2022-10-12" version="1">MKLSPIIQQIKDNVKKGLDIENSLYMLMMNAVIYTLSDFDVKYITFPNKNYKQNCIKFLEEVKKQPSNIDIQSIYQRMNFDLTGDDCFENSDEEKQNFFNKEQTIIQKGRKPTAYEDSLIQSLLNHLNEKEELLKKRFNNKKQHPENFKDYLDQMSPYELWKIIQDLKGGYYFFEVFENQKPEYGINYSYIDKMMMCSKSFEREYNQFKTHLRQQQELHYQTLKQEMNKLGPIELNTISEPICLIIKEDPEIECNCNQRGARQALRQWSYINQVIKLFKSNSDQEIQQYNYKGYFRNLIFNLEQYTNQVNQQFDYLIKINEILKSKVASLRILHQQLIQESQNLTKAQRLQDEEIIRLKQSLQKLQLEKSQINTRLINTENSLLKIREFSDQNNIINKTRLVAESLEQLEFENNQAIKMYKFQQICQLTQTENSFEYQLCTICRGENAIFQNIQQLKIPQITFEKLEMKQIEKTENSQLQNKFTQTEFKLPLNVIKNDQIRYLPSPGIQRKRGASTSYHLANENSILLRQISKSPPKQIINPVFKRLFEDSLNKKVRQSQLSSRIQGEEKNQWEEMLMLLNKLNNDQNNKDIQKKIEKSIESYKDQFDAKDKLPQLKLKDRPKVSEHLIDTLMKELQENKKVALQELVDQFQSILTVNKLNKSRDMYNKITI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TN72</accession>
    <name>A0A8S1TN72_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="14">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PPENT_87.1.T0240044</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20 14">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 14">Membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="14"/>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TN72"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000118">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000084">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF150">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 13 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="35"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="257"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="325"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="870"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="902"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="974"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1012"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1039"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1079"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="16">
        <location>
            <begin position="11"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="15">
        <location>
            <begin position="102"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="17">
        <location>
            <begin position="838"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="11">
        <location>
            <position position="390"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="390"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="390"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="391"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="392"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="392"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="524"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="565"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="587"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="620"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="699"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="700"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="701"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="786"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="792"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="812"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="815"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="816"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="816"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAD8153127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1177" mass="134797" checksum="53C989E1D813F44C" modified="2022-10-12" version="1">MERKLFFGVEQQLENKQFNYPTNFIQTSRYNLITFLPYSLALQFQRMANIYFLIIAILSFFKSISPFSPINSIAPLLFVVSLSMLRDGYEDYQKHLSDNELNSSPTTIWTNGGFVKKTWKDVLIGDIIKIDELEIISADLVVLQTSQDGICFIETSSLDGEKNLKPKQAVKETQTIECRQGIIECINPNQLLYNFDGTLFLESKKIQLTHKNFLLRGSKLKNVKWVIGVVVYTGIDTKVMRNSEEQKNKMSNIDKLINVRIIYILIMQAFICLILAIIYGINCDINKINFDYFSRNFSGYESYNGDDKIYNPDIPNCALASLMTFAAYFLLLNTLIPISLIVSLEFVKVGQGIFMQKDKEMYTAENDKYVKVFSTTINEELGQVQYVFSDKTGTLTCNKMEFKISVCGNEIYGDVSMFDKHEMSTFVGNQILRRQSTLHQRRSTVVNEKAGIEYTFSGAVIQSIITETDPIKNPNIDLNVDTYIIRNQGDLVKENLMLLATCHECVLELQDDGTFNYQGPSPDEIALVDAARRLNIIYKGITMGMMEIDVLGVKEKVELLFSFEFNSDRKRMSVIIKHKGVIKLYTKGADAIIKERLAPNQPFLAGIDKKLDMFSRKGLRTLCLAMRVLDERQFNQFSQAMNDTLGGNDTEKQQTELINQIERNLTLIGATAVEDKLQEDVPETLADFLKANINVWMLTGDKLETAENIGRSCNLLQEQMDVFFLTPGCDILSIFNQIADHVIQKPDTKRAMIIEGIVLAELSENDNLTKYLILLAPHLHTVICCRVTPKQKADMVRLVKNELGKITLAVGDGANDVNMIQEAHIGIGIYGQEGMRAVQASNYAIGQFKCLWKLVLYHGRQNYIRISEMILYFFYKNIIFTIPQFYFAFFCGLTGTSVFDEFFVSFYNTVFTFLPVVIRAIFDEDVFYTQQRKQTILGSKRITEGEEENDILRQSYPLLYYIGQKNTIFTSEKFFKWFSIGIFQGLACFFSFYFELNDTTFIKQTGYNNDLWFFSMSMSTAIMILVTLKLALNTQFWTIITWVAYLGTSLGTYFAYMWVSNIIPKSAIYGTTQMLFSSYAFYLSLALSVLSMFILDLLMFTIKASKDTLLNYMKRQARQKQQLDSAKILKLEKKLKEQISEMEMPHFELNQKRSLINSNLSQSQSGLKQGSSKDSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TNF1</accession>
    <name>A0A8S1TNF1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0240271</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="311768at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052591">
        <property type="entry name" value="CML21-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23064">
        <property type="entry name" value="TROPONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="153"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="507"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8153533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="829" mass="98581" checksum="1267A6026189F6CC" modified="2022-10-12" version="1">MGQIFQKHATQFEPSLIVKLKSITMRELFDCVDTIDKYYPQNCYLNLEQFDDIFATLTDNCIDLFHRLADQTGEDGKLSVHTFEALAAFAIFSGESFETKCMFVFRLFDFDLSNTLEEQEMVSTLQCAVRAMCKIAGLVIPSVSMLEKLGYVCFQMMDEDKNKHVDFDEFYEWTVQYDELQEFMLLYSGTQTYQYALKRMINVMDESEQQFNKWLRECKAPQVPAIKLQQNLYQIYKGIDQLSIDTLFYMIIEYTLRSKSIGQEDSSQQQKDLWAWKADHKKNQHLLQNISVKKSDYLDIIRAWNVYSITDVNQLSIINIKDLKLLIWLYEDEEPDYFRINQEMSIIDTNHNNIIERCEWMQYLCSEESMQRRQTPQVLMKKVFDNYDEEQCGLIPIIHIDKLLRDTFKQQMKKMKDFKIQFNNXTGEDGKLSVHTFEALAAFAIFSGESFETKCMFVFRLFDFDLSNTLEEQEMVSTLQCAVRAMCKIAGLVIPSVSMLEKLGYVCFQMMDEDKNKHVDFDEFYEWTVQYDELQEFMLLYSGTQTYQYALKRMINVMDESEQQFNKWLRECKAPQVPAIKLQQNLYQIYKGIDQLSIDTLFYMIIEYTLRSKSIGQEDSSQQQKDLWAWKADHKKNQHLLQNISVKKSDYLDIIRAWNVYSITDVNQLSIINIKDLKLLIWLYEDEEPDYFRINQEMSIIDTNHNNIIERCEWMQYLCSEESMQRRQTPQVLMKKVFDNYDEEQCGLIPIIHIDKLLRDTFKQQMKKMKDFKSIQSVNSLIQQLKVEFLKQVKKNNQSHFDWQSYYQFLVEATKTHSSLNKLLNLNRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TPQ3</accession>
    <name>A0A8S1TPQ3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0250287</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8154270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="293026at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8154270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1444" mass="171843" checksum="09E59FED55080092" modified="2022-10-12" version="1">MLHAKPKTQTQQALFQDLKSKKSSTSFSPINTLFGNQQTINIPNNLSIPLYKGHFVNFHHEQIRINDNCSLSGIQDGFLYFVVNEKTMQFYFFSPLHDDLNYYYNTDDKKYKFPIRFKEQLINHLDYITAVGFQQVDDVWTMAIASEFYIFLYTFTGDLDNFQMLKQGFNVNGEIINQVIFYQDNLIYGGSSGTLTCKSIDSAQNYTTQFKEKIKKQFNRIFKDLTPWSSQQGLIQLEVQEEFNICYGLFEKLDKDENVEDTFIIIYDIGLQGELFQEIITIKQSYLFNSNLEFKNAFDYHNLQFYHVFFERQYQTNKIVLIITTKDQLQLYLQFELQDRTINEINEYKLRISNSNVTQQQFIKLKPEFQVIQVKNPYMKIDTVIPYGVCLDEGRIQYKQKEMLTIQQNQILKSVYKEEQLSLFHLDNYIIAQFLDMAHIQYFRSLEQQITKYNSYNQIQYSIYRDTSKDAIEKIDLHGQNGHGIPKSTYNIGKMTVNRLFNHFDINEAQLYNLPEHFICIADHTIEFIVRMRPIDCFFTAIKVTHYDFIDSIDADFPLEKLVRAFGIEESCSQILQIIIQEEQNFYINVELQHQYQLDLQKAYQRIHYSENIQKFFDDNEKIPIWVTGQVYVRKGAIIKEKALKAYFALIKRSLINEDRNQKSKKVSSTTSLIWKIIAPLTTKKFINEKVYNLECNKPIESYSIKQLQTVFKQIEKLIKLFENESNYYQQVKNQRHDLQIEEQNQFQNYKNGFSKYIYETFEQSTRLDISRSTNYSDQSLAVSVNVDVEYNQMKVLFSYCKQIKQLLQFLIYFFGELQGIRNIINSYQNKTQLFDLSIKSVLSGDTVGNLTLKQLMIHIIKSDKTKFRETAQFMNYHFPDFFSDQDFKISLVQNLFDEILTFAAQKNLIKLNPNEQIHEKIKRILKLSKTPQITIDKVLEKKEIFLYYKDLQPELQDQLLEALKLIEEVAILIDHSYMTQCFQNYMYPFATFKFYVQYLAQKCQQQEKDQSNNEYCNCIMDLIQQFTNLIQYYLNPPKGLNKAQTKEILIESLQVLNQFQLIKPTEAIANNIIKYQLKEFIQYIDYNQEMDLQCNNDLEKLQMQKKQFLAKAVKGNHEVYPQLIQILLKLAQFSLSQCPEMNLEDIQIKLPIKKRLRYVNKAQEFIRTFHKNTQGIDINEVEKQAQDLSKQLFLQDIMYDFVISNEDENQSYHTEKRKILMDILDCKIILQFFEDNNIYFGQLLCLDYLEHSQGIQLCTQDKVEQLWINFLLSAYEESNGWPTDVLKRQMKLLFENLINNNKYIQLQKMTQTIELINTKQCQEQQITQLSTWLFFEILLSHDIREIDLARIYVKHYLDHIQILNNPLYQNLQIEQIQTYKLQLIQQILILFDALKKLKYDQQQTSSLINQFRNAFENDYRNLKDDELYDAKVIEQIKIRIDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TTE7</accession>
    <name>A0A8S1TTE7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0250270</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8154236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TTE7"/>
    <dbReference type="OrthoDB" id="308941at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035727929" evidence="3">
        <location>
            <begin position="21"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="328"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="496"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="271"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="344"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8154236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="592" mass="69155" checksum="415D326E12BC1E4D" modified="2022-10-12" version="1">MKTFIIITLIFICNSLNVNATSELEIGVIQFEEANGSQIYNQLVRFDTPFAKVPQVALALVEHTSTTALFAKVKNITQNDFIIQFFSAVTLNATYRYLATTDNEVYINCSNFRATQQAIFKYPQFQSQVNAIAFLVGYHHNGFVNISINQNSNIQANLSINTNDVQQTAIGMCLIVGSNLQFDDDADTPKFIKDKQDHYHLDQVQQNSGQSKQKIEESLTDLKTFFDQNQNRKEEEDEQFTDVSEELEPQIIEDLEECQQELNDNIVEEQSISDDFSEEEEDDEYFWNKQDKQQKSSVDFELQLTLLQDALKKKPQIQQQTIKELIKKTSSNQKQQKQKQVIKKQKDDVQKKNKQDDDEQNIKKNDNFKNVNTQILNNEEIDMKLQQKESHVRSLKKALQSVETAKEKELEVILPQIELEKQLSQDQELHNLKIDNKQQEILEVSKPIGYQSPLIDDIPKIEEIIEVKQKEKKFTQKLDGYMQSFSQDTYTQKVQKKKQEDQINKNESNQKEIMEQVQLIQLTGQSHSAKVVYEASEDSLRKFEEDFERRKQEKMKEIKQQLIPENKPTKVKFLSLAQEDEQLRSYKDFFYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TU49</accession>
    <name>A0A8S1TU49_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0270079</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TU49"/>
    <dbReference type="OrthoDB" id="307646at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF32">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="13"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8155414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="469" mass="54538" checksum="F0E52CA0CB943130" modified="2022-10-12" version="1">MQTEEKHSKCGEYVIIEKLGQGYHAKVKLAEKDGKKVAIKLFKTQHNTAQNIKSLANEINILKQLNHPNLVNLIEFSDALPYKKKNGTIIPRVCIILELAQRGELFQYVASSGRFAPEITRYYFKQLLSAMIYMTNKGICHRDLKLENILLDENFNLKVADFGFAKVMEKPKQKTNLGTPGYTAPEITQNREYNGTQADIFSIGVIVFILHAGSPPFSTATETDPFFKLIIHNKRDEFWNCHIKNRSDIFPQDFKNLINGMLAPNPDERFTLDQCLQHPWTNGAVATPQQIKIEFKKRYARIQAESQASQMKLKAQKQNIIRKPIGKFRSIIIGETEQEDFTETIEKQQLNLENRFLNKGQVIGLPNEILLYLDPNFILCYLLKYCTKFNSEVKKIHNSKYKINFVTNEEIDETIEYSVEFLDCENELIKVNITKNSGDYFQFNQIYKRIKNVLQENYKEYFKEEIQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TW34</accession>
    <name>A0A8S1TW34_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0280184</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8156204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TW34"/>
    <dbReference type="OrthoDB" id="298012at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="614"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8156204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="646" mass="77193" checksum="5BEE1DE61C09D77F" modified="2022-10-12" version="1">MQGNQGSQKKDIEFGSCPFSEAMKPPKGHEQYTDKSKCPFQQMQQQPEIKIADGSAEKKKEKKEKQPKGGCPFMSSEKKRNPPLAHLEEQYDTYYISPLNYLLDTRGLWMLAFDSKEVKKGPIKDRRKVFDTYPTYLKSTLFHDDENTKKLRQCEVAQRFFVYDKFREKGNKLLQKQEFDEAIRYYERALGCFRYLEVVEPPEEESDEEEANQQQDTSNMTEKERKELEDMKKSAKQYRKEQKEFKKQYKSLMTIYTDENVKYNGVEHIQDEADKEMCNSIMYGLYLNMSVCYMKMSHFDLARKILDDAGVIQKENSQYLFRYSQAILYDKWSTYKDLLKAKELIEKAISLSNVENIFKQGPGILKLMGLENAKEIYVEHAHRVMEALKKKKQWVQEIVEPLFLRAKEIDEIEQEMIEDGKVPYEEGVTDVVDPEDMIQQPNETDKQFLYRVCMPQLTQEHQEYEIVKEMVNKYYRIIEFYAEQKKYDQVKIAKQELQRLLETVQTMSFYMNLDLIDYENDEILKELVTKHQINFTDKKYVRRLIRLCRENVTEIFGQGKFNFEVFEYAMTDYFKKKREQEEREREEYLKQHPEPVKPKQESSFLKKTLFSTEFWMQMLVLLLVMGAMFYFNNNTGFIGKLFSLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TXR8</accession>
    <name>A0A8S1TXR8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0290245</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TXR8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8156914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="77" mass="9265" checksum="662A10C5BF7AF2B0" modified="2022-10-12" version="1">MRFRILISLSIIKVGVNYISDKGQPFYQQQDYELIIQETLFHKEEQERSVKQRQFQQEIKCINQVMNYKKVGQQSVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TZ93</accession>
    <name>A0A8S1TZ93_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="8">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0290289</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="8">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8157002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TZ93"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07834">
        <property type="entry name" value="STKc_MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000046">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000098">
        <property type="entry name" value="Mitogen-activated protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="40"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8157002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="425" mass="49275" checksum="9F9037A17145604A" modified="2022-10-12" version="1">MKPCQLPDDIIQHYSNFRNKKSKLKVSTILGSRFEIDDKYEILDNIGQGAYGIVVAARDNTLDAEDNLVAIKKIEKAFEHKIFTKRTLRELRLLRLLQHENIIGINTILLPKSREEFEDIYVVQELMETDLAQIIKSDQNLADEHCQFFLYQLLRGLKYIHSANVVHRDLKPRNLLVNSNCDLKICDFGLARALIPDLKAKAGVLTDYVATRWYRAPELLLSWRNYTQSVDVWSVGCIFAELLRRKPFLPGMDTKNQIELTFEVIGTPSEQELNMIPKEKYRTIAKGLPKRPGKDFNKLFPNASNLAIDLLKSLLTFDAKKRITVEDALKHPYLSALHCPDDEPIAVPVQRIDFEFEEYNMTLQQLKDCIYEEILIYHFKEFKDEYETKKRNSSSIINHIIKNENSKIIDPDADDDDNDSDEEPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U3Y9</accession>
    <name>A0A8S1U3Y9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Potassium channel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0320171</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8158813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U3Y9"/>
    <dbReference type="OrthoDB" id="6128189at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016286">
        <property type="term" value="F:small conductance calcium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015449">
        <property type="entry name" value="K_chnl_Ca-activ_SK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10153">
        <property type="entry name" value="SMALL CONDUCTANCE CALCIUM-ACTIVATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="282"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="3">
        <location>
            <begin position="254"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8158813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="462" mass="53640" checksum="F0BC4CBB69DD6741" modified="2022-10-12" version="1">MKGRTGGLTAKMNNQNQNRRQQENDQLVRKADQNKISLAEIFMANSQQLGGLEDGKVTKMYFHKFLFMEQVCFAFNLMGLGISVIQYDLEYEEFYNDDPMKDISLWLLWLVFVSSCLLVFLTVYRYQAHIEWMKSRKSMAPGDQIWHTDDWLPMLIELAVYCIIPLPFTKGIRVNFYNSIQGSEAYYHVNEILSLIMICRVIYLYRTLLTLTFWYNNRTQRVCNLYACESNYMFVAKSLLRTMPYTALFIALISLICIFGYAVRICERPLSRNDSTSNNLGVFQNALWNIIITITTVGYGDFYTRTDLGRFVIFIVCVLGIFVVSVMVVTLIESLKVTSLEGHAITVLERVALREKLKHEAALVIILTAKAALGYKNGTLTRKQHHQLIVRLKQTLINFKMTHRQHKTKQDENSLNEEITNQFSLLKNDFKQLMERQQVLISTNNLIYQKLGFDNNKSIVNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U5E5</accession>
    <name>A0A8S1U5E5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0340137</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8160070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U5E5"/>
    <dbReference type="OrthoDB" id="295621at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF9">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="567"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="600"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="407"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="446"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="412"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="458"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8160070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="639" mass="75124" checksum="89856AC9BF20B48C" modified="2022-10-12" version="1">MRKQFSFKPLSTHNDNQNLQTKSDSIGRFRSIIPIRKIQATCQFLKMKQDLELEFPQIPIQFKNQIPKTKIFTTKPHFNTHFPIKEDLSQFSKKIQQTIKTSFKKCLYQNKSNKVIYSNELSTVLYFNVTVTYEEQETFYQFIRRLGNIEKTQQDSSKQFKAKNLLKIVGVFEKYDTRQIFTFKIRSFYKMYLQNQIEWNYQHFYDLYPNSQFVDNICDKKNKLISRLTFQFDQKNELFKLHQQFCGILSLEIINNQQLNFIIENDYEDDQNQTCIKLIQCNKKIKLFKSHLQCIVNRLDNETQNNLLTEQEEHSFTLSYQNPQHFIRHLQFKVPDQNSIAIRFYNNYYEILNGIFEDTHEILNLQDNITMLEFEEPQENHKTTKMLQSVQQQQIQPKKLNFESIMKKSKHQPSITQEISPRSDHSQISSMRTPREGQLLPVLMKPQLENNKIKLRTSPPSYDDNISNSNNNQQYSTHHSKTVSQTDMVSINTPRSQRTSLAINLSLRTSMTKNSLKTRTLVVEQFKKSQQKANQLAGLLIQENKYNELVELFNNNPNLRIDERISNGNTYLMLAAQGGNLDICELILSKGASVNLQNLQGDTALHKAFQYGNFSVADLLVSYGAQVMVNYKGKTPWQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U6V6</accession>
    <name>A0A8S1U6V6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0310303</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U6V6"/>
    <dbReference type="OrthoDB" id="287129at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8158366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="126" mass="14801" checksum="9157596E80C23D11" modified="2022-10-12" version="1">MSFNNQNYMFEEFKVEEIQFIPEIRQTKSIVANPNKINKKYSICSYCLAYQENEDNTPYQCKNITCGKFVKIPVQYEVFQCPTCYANILHQPFSSIICSQCKKIVKWSDQLLNQASQLTQGIKKQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U6X1</accession>
    <name>A0A8S1U6X1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="6">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0320316</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="6"/>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8159099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U6X1"/>
    <dbReference type="OrthoDB" id="2420415at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061136">
        <property type="term" value="P:regulation of proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044635">
        <property type="entry name" value="UBP14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43982">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43982:SF6">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="3 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="8">
        <location>
            <begin position="94"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="382"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8159099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="950" mass="111929" checksum="5A10E723ED9CE56C" modified="2022-10-12" version="1">MQQSQLLKLIDDGLLQQELKNGYSQDEILNAWQASSGNRSIFQQILQGMKNQSFIISPITQQFNDDIQVAMAASLTLHQQNIPEGKIRIDKIPCGLLNVGNTCYFNSLLQTYYFITDFVSPIVKCKFDHINLQNIVDKRVVNSIQLLKNLQNLFVLMIGSDRQFVDPKQVILSIYDDFGKALPIGDQKDVGEFNNYFLSRIDEGITYLTQSQQKGQQQQFQQYYPGQSVIQNPTNNSHQQNKSFISQKSLLFRKSSVIAENSVMFKLFYGKFAPQIIIQGQGSKDCQQEIFNYIQIDVNSKSLIEGLEKQLNQVVEYKNDIDEYQQAIKQFWISQAPQILSFQIQRVEFNKQFCSFAKSNSPFLFDKEIYIDRFLLSNKKIARDIQIQNQQMNDRLKKIDEELEILNQFNGNVQIIQNLENTLQFLKLQNPNEKQNPFYVDNNDNKAVINSISQYHQYFIKKRDSLLKEKKETELKISQSYNTLKKQKYILQSILMHEGSPDAGHYFVYIYNIDDQKWYVFNDILVQEETEENVLKYAFGDQTNSKSAYLIQYVKEDKVKYTQNLNKKQKLFSTSIDTNNYLNDVYGSLLSQQQRDFLYQDNQKLKKEIIQQQLVTQIEIIVSIYHQYLEIANEKQIKYQQKQYKLLQYKPPLIENFAMYLKMKPDEKMFRWVILDYAIKKMMPTTDGLFGKPDFEENLKIQILAEVNKFVYNKPIQPIPQNQEYQQLLDEYKNVLKLQCMIAYVLEQYNNNNLKETVIALHFYMKLAKLTNQQSFFYNLALQLKPLVLLGIICKIFYQKIICDQDILVFKLIIAFQMQEHFLYKDLPLWSKQLQNLIGVALSIYGQNEKQTQDVLKPLIQNKKDFEMCSQLDTSFQNIDQYYQSIQSIYLNKQFESDLDDYLQDNSMNSLQTFLQIKCIFILFDKIQKSNQILDKKDIVKIIEENPQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UCP8</accession>
    <name>A0A8S1UCP8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0370207</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8162114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UCP8"/>
    <dbReference type="OrthoDB" id="299152at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="92"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8162114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="334" mass="39728" checksum="28CB55FC2B167B1D" modified="2022-10-12" version="1">MSQYERIRPIVFQKAPEVKQVRSNSLAVRSSPSKCQLDLSFDLQVNSTTNNTNQVLKDINNYKKLYVSESCQNNNKENQLSGKHFSFKADESQYLKQRIKQLESQNNNYVTENKKLAHVLDQQIQLNQSLQEQQQSKNQIIRKLEDVQKMSKYQQSNNTDLKQINEQLIISRKVVNDLEEKLQIVLNENQKLSELNERFQFTENQLKIEVEKYKSKCSILEGKLKQQQDESKCLELQRKIKKQNDQLELLARENYQLKQQLTQNNNQISQQEDKQFNNDKFKETIQELECENQYLQQMIGIDQQKMNNLEEKLNLLTKENQRLTEIVKNRHKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UD61</accession>
    <name>A0A8S1UD61_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0370288</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8162274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UD61"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052603">
        <property type="entry name" value="EFCB6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20875">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20875:SF0">
        <property type="entry name" value="GH12158P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="178"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8162274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="246" mass="29173" checksum="5F768D1DF28A7306" modified="2022-10-12" version="1">MLQQEENNELKVLFIIQNNNYEQIIMQQNNSKVIVLKQNQEAKLVNALYEMMYLNKQLEERKIQLEQHDDFDLNQFYSFFDTFNQKKITRLDFELGCMQLGIKVKKQQINLLFQRFSEDNSFLTYQEFVNVISCSNDPLVRIVTKISIKTMTKFKELIAQILLTEEKIQLVKERLAENSEFSLELAFLFFDKLKVGTITIDEFREVFESYNIQITNQEIESLISIYTKKESRVSYGSFISGMNPIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UK10</accession>
    <name>A0A8S1UK10_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0410058</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UK10"/>
    <dbReference type="OrthoDB" id="293868at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="70"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="315"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="352"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8164487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="390" mass="47221" checksum="158B509F32046A1C" modified="2022-10-12" version="1">MLSQKTLQLLRKYLDGYLKLENEIENKREQLAHNLYFEPYTIFCRLDYKKILHIDINDLKVYFLDNNIIDCEDDLQFIIRKIDIDKDQYISFSEFSRYILPHRDQDLNSLANSRQSYQLLDNKLPSLVEKQLIEVFLAEIELNKRMLQQMQQLKAQPDWDKITAWESVNSGRRWDQKGLQQYFDESKIQYNFKDIENFIYRNSKERDYRINYSDFLYLVFAHEDNNVNYKFFTPQKNQKLISDKKSNLLPKTNFRTNESTNLKSDKFNKHLQSQSHHNQIQNHDDIEVSEFKINEYEDQNILQQLLNKLKIQNQLKGHFLKQLFLQIDENKKGFITLDDLMNYFENILHTPKQIEELIKLVKILDKNKDGIISEKEFCEQIVRHVYIQQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ULE7</accession>
    <name>A0A8S1ULE7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0420109</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ULE7"/>
    <dbReference type="OrthoDB" id="312951at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="116"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="321"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="489"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8165234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="517" mass="61012" checksum="E0135005403DEE41" modified="2022-10-12" version="1">MNQSWKMNASIETVLLKDKGSATALLVKMRSTDVAQELYDLYNYFQDIQKQNKNLQDSITTLRSAITNLLSLSGRKCQGGEFDKVCLNDLEMIQSIMNRVRVDIIKQSEDKPNNDLNGQQNQIDNLQKKIYFLEDSIQQLMTNYNRLSNINNQLFEQNYTQINNLNQLQQKYTQQNKKTSLILFENQLKSVNQNSINIEIDCQQFREQIININNQRQQLIELMQETLARFSHIQSENKVQQNMKNQSLDVNDFKQIINLIKESQTSINKYVEGIKKFVDKINLNPQNLNNLAIQDLLKKLSQNRECFVYIQNVQNMTLKILDQISQDYFQQQEKNKELEDLLGKLKKENIEIKEQLQDQINQLKEYNKKEQQLLSNQMIVKQCLNISNKYNNIFKEFNDQVKKEINRFSNLILILFPNVNEVSNISPDQDLLQDIQSFINSIDNQDIKNDDDSQEQKWQRSIDLFKKIYKRIPNNSIVNQYIIKSVESIVEIKGIIQEYKENVKKFKKEIEMSVQTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UPJ1</accession>
    <name>A0A8S1UPJ1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0460069</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8166791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UPJ1"/>
    <dbReference type="OrthoDB" id="295416at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46539">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ATL42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46539:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ATL42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035806078" description="RING-type domain-containing protein" evidence="10">
        <location>
            <begin position="16"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="381"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8166791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="437" mass="50937" checksum="13A86497208F1E1E" modified="2022-10-12" version="1">MWFILQFLQFQMIISLETVIQISENAKSWSEVQMSSPNNTYILNIANTVTYPYYAINVELHKQDDTFSLHYGIGKPPEFSLNLSEITEVQGFDIYGYLENRFTHFILLSSQDFHKGQVYISTSSEWTQNYNITVTATYDKLCPNNCNNQGSCQDGKCLCNKYYIGNDCHQQATYIEQNKEIFLNLENTVKYAYVDLEESVNQTMSLIVKTNSSEGVDLYIMKTRVLYIPALEDLNRKYYDLLVEGNEVSYLYPIEFGLKDRNDISTQIQKRIRFIFKQKSLVEQNIQLSLDLINQRTQNTISTTTVIIIVASIAGIIMILSLSFAIGKYFRNKKIKETLAALAMIRQMQHEKKYSAKSFDDEILEQLPQIEKDNIKNTDVCPICLDLYINKPDLRSTKCKHLFHRECILSWIYINKNCPTCRCDLKIHMNHHRNQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UQA4</accession>
    <name>A0A8S1UQA4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0460102</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8166855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UQA4"/>
    <dbReference type="OrthoDB" id="297252at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8166855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="199" mass="23446" checksum="D4DBE3ADFDA96266" modified="2022-10-12" version="1">MNICAIRHIPYSNKNNRRQIVVDQNRAKSQQGSRLNSATHYRCSTQQSNNQTPNQGKLQFYEIQPYVGQLIKNQQQQFVSTQLTKSPSFRVKNYHPQDIIKTQTQQSSQNQLQSNIEKIERMNRMNFLGDLLQQMDGKNQQTRQSIVTELATVPKNKIDIFNIKNQQEQNIEELHFYLVESQQRIKQHACVIEQQKYHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1USN3</accession>
    <name>A0A8S1USN3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0420222</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1USN3"/>
    <dbReference type="OrthoDB" id="283003at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8165456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="273" mass="32611" checksum="6D2DE27AC7D3ADEB" modified="2022-10-12" version="1">MKICFNFTKQTLCNRSFSQADVPFVAYQAGLSFNMDLNSPFRWTPKIQKIEQTPEFLKKMKYFQRLTIMEDHHYSELIDTFLFQALKNTDFDYYYDYKCESNPYCPFYGNIPFLIYNKVNKYPIIPVMRTKPINAFTLVAKEREMLITEAVAAGLWALTNSQVQKLDRVRMLQTDGNHWRMLELCKNEKFKKTGIYCQENDSYSKIYYDYEVQQVILGLIRYSLNQMDEKEQALNLIYDFVDERRYLTHEGAPPKATAKFWKFVPQSIKNFFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UUP1</accession>
    <name>A0A8S1UUP1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0480109</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000048">
        <property type="protein sequence ID" value="CAD8167943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UUP1"/>
    <dbReference type="OrthoDB" id="307112at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="217"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8167943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="226" mass="26702" checksum="7FC04DDC05B6B0FD" modified="2022-10-12" version="1">MNQTKISKVDILYQRNNQAILEMPKDYRKGHFMIRKNEIKIEKEEVQPPKKKDADQNIPKDYYQQRKKNILIIRDDREKQPYQSQKFLDDLPQAQSLILFKNRDKYSLSFVSQKSEVYKHMSEEFQNKIFKDIDKGLDARKNQENRDKVDIRVKKQIAGYDDVLSGSDIEDPYSSKKKQTKKNKKEKVSHKLDYQSDGENPKKKVKKDESSDDDQSSDSVQDSSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UVG4</accession>
    <name>A0A8S1UVG4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0440167</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). The elongator complex catalyzes formation of carboxymethyluridine in the wobble base at position 34 in tRNAs.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the ELP1/IKA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UVG4"/>
    <dbReference type="OrthoDB" id="40048at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002926">
        <property type="term" value="P:tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056167">
        <property type="entry name" value="A-sol_ELP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056164">
        <property type="entry name" value="Beta-prop_ELP1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056165">
        <property type="entry name" value="Beta-prop_ELP1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006849">
        <property type="entry name" value="Elp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056169">
        <property type="entry name" value="HB_ELP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12747">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12747:SF0">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23925">
        <property type="entry name" value="A-sol_ELP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04762">
        <property type="entry name" value="Beta-prop_ELP1_1st"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23797">
        <property type="entry name" value="Beta-prop_ELP1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23936">
        <property type="entry name" value="HB_ELP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017233">
        <property type="entry name" value="IKAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="ELP1 first N-terminal beta-propeller" evidence="6">
        <location>
            <begin position="18"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="ELP1 first N-terminal beta-propeller" evidence="6">
        <location>
            <begin position="144"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="ELP1 N-terminal second beta-propeller" evidence="7">
        <location>
            <begin position="324"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="ELP1 alpha-solenoid" evidence="8">
        <location>
            <begin position="593"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="domain" description="ELP1 three-helical bundle" evidence="9">
        <location>
            <begin position="989"/>
            <end position="1135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR017233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8166476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1172" mass="136391" checksum="99658165F416BA68" modified="2022-10-12" version="1">MKEISIQCQQSYEISQPLAFTFDELSKILYVIDQESNIIKYEYNNKSLSIQTTYKVGQQIQNTSQILSFQYLMENDQLIIVYRDGAIIKVQKDDAEVVGQFECGVLGAAWNPNQEQLVVICGDGKLVSFDAQIEPIKEINIDGILDQLVSISFKNDGKFFSLNYAIKDGRICETYDVQLEKFRSPSKSDPEGGLVQAIFEKPRNLVNTVSWQPNSQFIAGIQEKQVIFWEKNGLRHLEFNIFEDSTNVKWSPDGVILAVQQGNKITVHLRQNYKWYAKRAFKFEKIIDYTFIENNTLVVFNQNNVSIFWLNYQFNNATMGLSTDYDTLLVSDYKKGVIPPPLCHYQLKVDSQIDLVYFDQLIYVYSFDGTLTIFDNANQLGKIKLDIEYAHKLICYQDKQQDVIFCHAVFVVHEAESDKIVDVVISPEFEIINIWRKEFSQSTQVTALTKKGNQIILHSRFGEKFLYDQSQEESLSEFDLANKEQNVYDVKIQQHNEWLACLKSNSKLTLNNQLVTTECTSFAFFDHFLAFTINTTDQFHNLYILDLNKPIQIDKKSLNISNIERGAKILAIVSLDRLIVQIPRGNLETTAPRVMALHLCKQLYENKQYKICFEMIRKHKLDMNLMFDFSGELDVQIIEQLSEQYLQLFIQSLNNEISFELPYVLTAQEYKHQKVLIVDKTLGTSKINYVCQKLIDNMKEETHILTIVTAMLKKEPSEVEEALLKTLKLKQIEQQHEEVPPHLNPDTHKPYKKHHKIKSEQVLEYICWLADANKMFEVALGTYDFDLVKQVAQFTQKDPKEYLPMLERYSQIKDPIDMKSTIHIELKNYDKAIKVLSEGNEEQKQKSIELIRKQNRFRIALEVYRNDQGMMKKVKEALGDYLNNQKQYHQASLAYESAGLYEKAVQASSEILDTKRILSFDPKEDYLKNYNQILLAAGRWKDCGQVQEYLKNYEQAIHYYCKAEEWEFVAQCLRHQQINIDDHLQLAFSLKVNHLLNQQHLFIQKLERLRIVQDQKKEHGILAPSQVNADFDQMSDVSGKSGMSKSSYTMSVTTGVRKRKPKEKSFLNRNIKEGSPVEEEYLIEFLKDIQAKSADLINSIKKFQNYLIFFNQPKLSLELSAKTKEYLNTIKPEIKSLLQQQFEEQNQQAIDLYPYKSVVPSEFNQALDDLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UXX1</accession>
    <name>A0A8S1UXX1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0490062</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UXX1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8168389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="526" mass="61627" checksum="2049065B8FAC290C" modified="2022-10-12" version="1">MQNKSYPSTHYTKSSLSKQSIHQQVKPQKSSFTRWLQRLFCIKEINEQQYITNMMEISNYDESQQPTMKIISKFSQYDEYFSIQDPIRIISGSDSQFPFVVKFNEEKLCEIDDQYHQQIPELNEQVVVLITQNPSLALINYIESKASYFIIYLSTPNPVIQDYIQQRSKQYSKPELIIGKFSIKKSLSLVDQYIFMPDYSTEGRQQVVDTVSQLLKLIPNEIDKFTFLIKLQGNPIQDIFSISSQMNEAFIQKYESEFWINKILRLSQNRNAIVLKQRDQKKTGFEKSQYMMLSNNINQKKGFNLTVENTELIMNLTKYQQGNVITHVLKCLIYQKGIEGRNNNTIDQAKLFCTLIFLIKELKRFSQYDQEDFISLMKIIQQSSLLSCCLCINLMKQEDLKELSQIKMHYKRFLEILICSGIITNEQDSYVLQSIFNKCLNCPIYDISIPKSEHLNSGENYTQQLIQNEDIGDKIYSIDYDFAKSPKSYQIVLGLQQDLNYSELQIEPQKRFPQQTTTSISNSLNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V938</accession>
    <name>A0A8S1V938_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0600200</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000060">
        <property type="protein sequence ID" value="CAD8174048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V938"/>
    <dbReference type="OrthoDB" id="306901at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8174048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="60" mass="7076" checksum="7B2045BAEF9C8D70" modified="2022-10-12" version="1">MNKVIDKIENKNLDPIIVMKNREYLIDHLVIEELVKKTKTLNIEEDLDSTIIICNSDYED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VA59</accession>
    <name>A0A8S1VA59_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Calcium-dependent protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0570213</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VA59"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000526">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000708">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000771">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000660">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="11"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="298"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="334"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="417"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="453"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8172672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="496" mass="57735" checksum="B8304AAFDA7B4A48" modified="2022-10-12" version="1">MTDKRIDKYIFNVKDRIGQGSYAEVYRGTNEKTGEKVAIKMLSKSVINSDDYLREGLIQEIKIMQKLKSPNIVTLLDVMETNNNYYIVQEYCDGGDFDELLKKRKLLSEKDAIKFLVDVLNGFTQLIKNGIIHRDLKPANILIDKQTYKLADFGFAKCVDNFKKTMMASMVGTPLYMSPQILDHKRYNSKTDVWSIGFIFYEALFGKTPWTARSPAELLKNIRTQPLKFPTDKNQVSQETQDLIIGCLQADENKRLSWEEIYKHPAVSQYFTDFIKGNSKLEDRAQYLINDIRQMICKEKIDIHKLFADLDMSKDKALDVNELGKFLMKVDKDITREEIEYIFNKFDDDGNNQIEFEEFKKWLEDNQIITNGNAQQQQRNGGSHKKLSILPHQLKSQGSIEERANYVIEKLKLSIQKYKINLLDLFKKFDKSADQRLDKNEMGLLLKRIEPNITQEEIIACFDFFDENGDGEITFQEFQTSLSEEVGKKRQSIDNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VAL2</accession>
    <name>A0A8S1VAL2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0590213</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000059">
        <property type="protein sequence ID" value="CAD8173611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VAL2"/>
    <dbReference type="OrthoDB" id="426737at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="276"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="326"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="354"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="7">
        <location>
            <begin position="456"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="789"/>
            <end position="826"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8173611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="934" mass="110895" checksum="0CE7ACFE94CD14EB" modified="2022-10-12" version="1">MFQITNNDELQEPLETNMNKSSKVLLWGRRCQRLSFGIENPIWKFKAFKIIILVSRFLFLVTQQIFRKDLKNISKSEFNLIHDSSSDYNFFKYRGYLPKDKPSYLLMKIHLWFSCIIQQQNTFLQNNHKKWLLKPDDTIMIVWNVSMIVIVTINVFYVSLRISFPEIRELSLIGKELIFEQIPVFIFFLDILLKFNTCIYFRGNLISNRIKIIKHYCAEGLIIDLLLVVPFFIGQLLNFNHLDFVIVLKVFQLSGLFSSLFNRLELTSRQTAIFDLFKMISFMILVAHFSACIWHILGQWGQWGHEDGKTWIKVINIQHESWLDRYVVSFYWSIVTMTTIGYGDITPVNLTERIFVIFMTMISSATFAYTVNNIGGIFQDFSKQSVQLKNNMNELNRFLRSQNVSDDLQIKFRRYFEYLWSKPSQKVIQFADLIPRSLKDQMIVDVNIKIINQISFFQNFSQPLLNKLCMNLEEKQIQSNDYLFTRNKQSQYLYILVSGEIKLQIQLRDKIKLLQNIDVPSFIGQLDFFYNQPYSYDAIASKTTKILHISRESLHNIYKDFPFDYEKFKQISEDIVQKKQLSHISVTCNTCQSNTHFINSCPFLFGTPNKAKAIYNYRKFTPTDRKPCFQRHNHSRKMYALKHQFIVLESILNYMMKNEEFIHIEGFKELQMQTQQIQNNQFTFNQIPIGLSVHHSNNSVLPPLLINSSSKQSQSQRVLQSVGNLLPPQVDDSPRTFRNNQIINTTNLRNYHKLSINHGSQTELPKIEDEVPTIGKQQKRNDIESLCVINDNQQQQQQQQQQQQQQQQQQQQYKQLNQQHFQQQQQHQQQQLLPQIQINQIGLRRKSVRLKLEESSEDENDDSDKIMGQYIDIFQQFERMYEFINYYPHNNISIVINKYQERIFGLQRQIKKRKSRRQETLLHLLKQAQGKQRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VAL7</accession>
    <name>A0A8S1VAL7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0590210</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000059">
        <property type="protein sequence ID" value="CAD8173605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VAL7"/>
    <dbReference type="OrthoDB" id="311673at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007783">
        <property type="entry name" value="eIF3d"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12399">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12399:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05091">
        <property type="entry name" value="eIF-3_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016281">
        <property type="entry name" value="EIF-3_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8173605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="515" mass="60219" checksum="70427EB1CFF7CC52" modified="2022-10-12" version="1">MDSNLQNLIKNYAPLSDFCPDPNYQFEEFKDRLFQPFSRQEKISRCCDFSKQTQVQKDLEDEYETVGQKVVKKPVQKKIGFVPQQQQQVVYQSYYQTGKKSKMSQAAKRVMVYKKCRFKDSISIKTDWISIHETQKQNIEKVQYSMIDIKEIQSIGTIKKYNKEFDKVRPNYEKKISIIGGEVISGSVQDDKYFKQLIAERQGTEEIPTIYTTDKLLFAVQTLKYSIYPWDILVTKKGNSYIFDKCPQNRSELTYLELQTINENITVDMPEDEKTVRNYCEESTIAQLSWQLLSTLNQPVLFQNSLQNDEETEQNDQAEKYLFSEDLCFKYLEVSVKDRIEKAAKGEEVRAGQERFKVVVRTTVEAENNDGQPVLVKSLNECETPPDWKKSLLSQAGITTNTAQVYNTNNIAKWLCQAQLLECEEIKVGYLARQNQKDQDKHSLLLVETFTQRDLAAIINFKSYEIWQSVRYLVDYLKTQEDGVYVLLKQAYKQSVRIFNVRDNDELQEEDEDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VBG0</accession>
    <name>A0A8S1VBG0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CBM20 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0560181</name>
        <name evidence="3" type="ORF">PPENT_87.1.T0900214</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000056">
        <property type="protein sequence ID" value="CAD8172226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8188113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VBG0"/>
    <dbReference type="OrthoDB" id="550577at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001070">
        <property type="term" value="F:starch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05467">
        <property type="entry name" value="CBM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002044">
        <property type="entry name" value="CBM20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15048">
        <property type="entry name" value="STARCH-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15048:SF0">
        <property type="entry name" value="STARCH-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00686">
        <property type="entry name" value="CBM_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01065">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51166">
        <property type="entry name" value="CBM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM20" evidence="1">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8172226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8188113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="183" mass="21033" checksum="6EFBFC5767AC47D5" modified="2022-10-12" version="1">MAIKLNFKVRCETTLSESVCIVGSVKELGLWNPSDSLQLSTNPDIYPFWVGHISVDVNENQLIQFKAIITKGHQVNWEDNDNRVIQIRYQSQSILFSFNSQLLQVIQIQSLYDLSDDESIKFDKIKKINLQNVMNPFDQEGFTSESDQESDRLSNLDSHGLSPIMKSLSIQYQEESFELPVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VBI9</accession>
    <name>A0A8S1VBI9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0600120</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000060">
        <property type="protein sequence ID" value="CAD8173893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VBI9"/>
    <dbReference type="OrthoDB" id="10251809at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14733">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051568">
        <property type="entry name" value="LZTR1/Attractin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46376">
        <property type="entry name" value="LEUCINE-ZIPPER-LIKE TRANSCRIPTIONAL REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46376:SF1">
        <property type="entry name" value="LEUCINE-ZIPPER-LIKE TRANSCRIPTIONAL REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="377"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8173893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="530" mass="61355" checksum="61D6F85F9A6AD4EF" modified="2022-10-12" version="1">MDYCIRKTEMTDNKEDDVTFASYEWENIKATTASQLPTCRNCHTATTFKHYMIIFGGKEGEGRKKFCNDIHILDLKRLNWTSQIKVSGQIPDVRMGHSAQNYYDKIIYYGGWNGYTVLDDIIMMTPSEQMNVVCIDWQYVKSENTPPKRQFHTANICGDVMYIFGGGDGKMWLSDLYKFDLVKCYWAQVETTGQKPQGRLQHSSVIYDHKIYVFGGEPDRSHQLNDLYQLDIGNNVWTRLQPKGSTPSPRVSASAVMMNNKIYLFGGYDGQQWRNDVFMYNIIENQWEYIVINDQEVLPQFRGQTKENISSQQSSPPRPRCRHSAIAYKNTIVIFGGNDSEKSYNDVYMLKQQSTIKLAESTLKQDFSSILFSPILSDITFFVDNQEIHAHKIILASRCEYFKTLFLNEQFNLGDKLAITETTINVFKAILQYIYTDEVLIDSYIVYDLLALADKYMLQRLKNLCEDHLIKNISLKNVIEVVNLADKFSAQELKANAMIFLLDNKQKILNTQDINMLSKEILIELLKFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VF85</accession>
    <name>A0A8S1VF85_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0610166</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VF85"/>
    <dbReference type="OrthoDB" id="10315991at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="252"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8174559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="525" mass="61553" checksum="B6DC111818A64DBD" modified="2022-10-12" version="1">MKKSNENERIAQKIYASQIQKLQGKILEKKLSKVCSKQKFESQSQNISNNNSPVMGRQQSFENLQKVNGTFINPTLQVSQQKLSSILNVIAGQQTKPKIQSLFKNEVMDTVKITHKLSKNQSMIQIDRQSPQLTNNSSFHRPNSSVKIKNINSLSYEQSQLQKIKDNIKQLLQQRDNNWQQDQKEMTFMIKLNQSLNQLLSIVFQDQQNTQATSTQDTNSGVQLDHFFKRQITILQQSYNQQMEKKKLENILTSIKRKHENVLIEYNQLLEQRKSEEDKVKNLESQIQGLQSKIYTQQMEIKNEQEFLELKYLVQGQFEAIQKLLQREQLTKIFLKRVGDNSILEIFDQFISNAEQTNMDDTEGNLNIDVLPSNKLISQKKQAILLDHMSLPEKIISQYEENYKFQLNLCDSLLADDSRVNDSEESSFGYMGKEQATEISYYFNNAEQLTQVQNKRSRIQTISKEKLKINMMDVQLAQSVFKHQKLVEQQQQQQQQQQQKILPDYLMDNENEESEENFCYQEQQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VFJ6</accession>
    <name>A0A8S1VFJ6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0630017</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VFJ6"/>
    <dbReference type="OrthoDB" id="307701at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="99"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8175361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="192" mass="23291" checksum="CF61BF9BEBD6500A" modified="2022-10-12" version="1">MFINTTHTPDKKNSYLNKFNKKRIQTTDKENKVQNLSNLSTNDDCKSYCDSVDYFRHENQELKKLVFQLKFEMKSQLESCLIEQKHNLKSVVQKYKSFIDEMTTQKNQVSSECDNWKQKYEQQQQIIEQQRLQIYNQSQIIQQNLSTIQNIQQEYQQELIQNQMDVQQIMYLKDQTIYELQQIIQQQNQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VH77</accession>
    <name>A0A8S1VH77_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0640055</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8175873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="295848at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19757">
        <property type="entry name" value="Bbox1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF73">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="874"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="983"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1020"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1064"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1095"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1298"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1343"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="476"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="9">
        <location>
            <begin position="1490"/>
            <end position="1536"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="741"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1387"/>
            <end position="1439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8175873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1774" mass="211187" checksum="4E9260F0649E0AE3" modified="2022-10-12" version="1">MFKKQISSWTQFNSPKVLNNNLNSPSIKSDQQSKDEIEEQQDMIIEENFIKNVGSNQNQRFESDFTVEDQDKRNILHQTALRQDNKVLEILIEDYKILLKDNKKKFKYQQQKKIIPQKMEDDEEIIEIDFDFDDNDGLEIQEQIKKYVQQKDMFGCSPIQICCFLNDPKLQNNRQECLRILIENGSDVNCINPHSRWAPLHWCAFYGDQVSVQFLLKKKAHSFLCDNNGLYPMDLAGKNGHQRVLQLIIDQIVAFLTECQQFWINELKGKNESEYIQFQEICHQYEAELQNPLLYTKILYWCCLYKINHHIKHILINLPRIYIMFPIKSLEGQTCLHACCSQNNYEALKIIIKFEILDLSMRGPDSNKKKKPNKKFRDRENYYSKSLISYLEQICRQELPGITHILENRDSSIYKERYYHELERYQIQDLKYKEELGKLIMRFMQQNELKNDKQYYDKTKELFCKNVLSIDVKDNHGNTALHLASLNGEQKIIKFLLNKYADPEAENNEFFRARQLTKDTATQIYYDGLIKKKKYAQNQNLVHQSIGEIFEKHKQKREKQKVSFEDQYNLMENIVAKSKFYKSQLKTKRQIQAQKSIKEQKTLSLFNENELEKSPDFLKSSVQRENQNFLHKTAKPKKNKNQIIPLDISNSIQQNQKNNSFDCLEQLILFSSQLFVPDIVLKFDHSVKTNLIDIFKSHNLQEIEEQKRIIDQQINFQLDLLQKAEFEIYMMQSFISQQQVYIMLRLKEEKLERLAEEMEMQIKLIDSYDLEQFKSNQRNKFEPFRSSQRQKIVYDHLIKSINLESLLKSKLVESCYAMHTSGGIQIIKRQWQKLSFGFIPQPISQIKDYLNEGRGRNFTSLTTMRLYFGEQISFYFAWISYLSCVQLIIGIPGLALQLYAILYDFHSDLIPYWVLLVTIWSTVQIELWKRKTSEINTRWGCIDQMLQTESSYYEGPLKDKFSGDEEINFITRKLTKHQQITKMAIYFILFIFLLAVFLFCSLAIVYGIDLLKQELIIYKGFIFLLGALQAVAIQILNILFLYFSKWYADQENHKFELSYQKSLIYKNVFFRFINSYMPLMYIIVTSNKYNLEDIFYFIIPLVLVKKAYYIVVNFLIPVLLLRGRIRNYFQKVKEITIKQKKIRLANKQHLQYLDEIEQFWDYDEFKKNYKLDIQDNIDVDLLESAARPVLKIESEEVLEEKILEETKNHKFKYMPFETKIDVDAIELNSLKDDFEGTLDYFMEAITDYGYFILFSAAFPIGPFVGIVMNTIDIQVKLYKLIYMTKRIKSERVPGIGQWLNVLEFLSATGVFTNFVLLYFKHRYATLRMFTDQVDYVIENDLELWYFMSCVIAISLIKILVRELIPDRPDWVIEEIDKINHRELVEQQQKTQIKLKDLDRHYKELKDENKKLKKEQGEIEQSTEIKLKQLDTEIAKLESQFFSISKYKKLDKIIQTEYDMIVQSVMKIRYYQIDNALIIKRMLQFLQANFSNLSICQECGKNPTILECIECTEQFCAACYQKLHTITQQTQHNVNLMRKKNEILEQIELNVVIDKPNQKMIPNELSKQLKSQIKVSEPKRCWKKIEYFFIPLQLQNGQPKLNELYNQFGDYYLRGPGLEFRDFKIYVEKIVPIKELILIEDSTLRIEDKIFLNRIAFCLFRKKKNLAEIQLFLKNCSTLQIGQLEQKVLLFFDLLDLNEDEEISKDELENQFLLSFVQDVRTNKTILEMIDSFFIDKIKKRDKKEITQKFFNKVQRNNDFQSFLESLLQVQGLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VJE8</accession>
    <name>A0A8S1VJE8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0660048</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000066">
        <property type="protein sequence ID" value="CAD8176881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VJE8"/>
    <dbReference type="OrthoDB" id="304489at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8176881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="167" mass="20000" checksum="B22E973F72517EB8" modified="2022-10-12" version="1">MGKKKPDSFLSQMSKLFNGEKIIKLTIKRNLKEQYPQLRKRLKRKQKQQLLNEIKTANDVNEIIIRSQTQKLTISTSSNKQQINKFADSLSNIYRLYCHDQQKQNKKKPVLLPTQPNAKSKTQQRLKAKKLRNKERRQKRNELQKLKKKELQRQKTFASKKREDKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VJL1</accession>
    <name>A0A8S1VJL1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0670056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000067">
        <property type="protein sequence ID" value="CAD8177167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VJL1"/>
    <dbReference type="OrthoDB" id="308029at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="768"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="768"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="781"/>
            <end position="790"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8177167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="790" mass="91730" checksum="CEE3C025FA309C8E" modified="2022-10-12" version="1">MLGKKQSSFIGFNPFNKIVKENPSISTKIINKLKSLFYINLNPSSCIKRELISQQTIYVPVDLEIVGAQTIEHDSIQICNSQNSIKQRRNKFMSFDDHINNQQNEKVQQNELSQKVQQKENKHMKKFNQLQYSFDPVFPEDIDIAQTKQNNIIHPKRKLSSQQYKKRKLDQKKQQVGIKFVFKDNIQDQIQDSINESRIKEVPEQMTDDSLKDNSLLVSDLKKEPKNKEMIIIQLDDEKQKQIEPPMFTINEIIVNQSKGLKKSQSMINLNEQESKSITYSPKIHENSQSRKHSDIINKINETKQIDPFNDQVKKDIIKNEKLTEFSVQPQQNVINFLVQGSEKQDIIKIKNSNTQLLKEVSEEKKISEQKERNINNFSDTPLFGFGKYREGQQEQVIQNKINVHSSIFSSYDSKDLNLQNSSNQSQNLQQNQFTFLQNKSNISVIQDQPSNSNGNNQKNILNRQEENKQNNGIYNNTSNNQNEQFRPMKQLFQNLIFQPQSILGEQKKEENKYDQSEDNQPNSIGLNFQLKSNNESLKQQTKNEQNKCNFSSSDQLIKQVTTPVKQIGNPFLQQSPKIDQEQIISYFSNHSQQQNRNNQDDPAKIQTSQFSDDLFKLQNNGTSMFNPQSAQSSLFSFQQLSNQQNQSNQFLTNFNVFQTSVNNNMNINNNSMNNNNNMEITNTSLPNQQFQFKQQLLQTQNPLGSFNQPQSESNNFYPQIQQQISSGFNVIQFNSTNPKQQNEHQNQLLQLPQTNCIFTQDSSKLTNQSQSLNSSFQSRNAKRKQRIDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VLV7</accession>
    <name>A0A8S1VLV7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0690040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VLV7"/>
    <dbReference type="OrthoDB" id="323142at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8178200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="123" mass="14781" checksum="ACDFD29CDD5A85A9" modified="2022-10-12" version="1">MEQNQLLVVEINKFCQWKDLIDNCGMLYKRFLRMVIVLYLQFYTIDHSTELYIKSKELQVQGEVQECQYYFTQIYLPDNFYFPKMAILQIYCNQILILKIGIAYLQKVLSLIKVEILETFLGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VMF0</accession>
    <name>A0A8S1VMF0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0690151</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VMF0"/>
    <dbReference type="OrthoDB" id="441412at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098719">
        <property type="term" value="P:sodium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000244">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110:SF86">
        <property type="entry name" value="SODIUM_HYDROGEN EXCHANGER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="9" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="121"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="237"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="275"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="354"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="387"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="11">
        <location>
            <begin position="750"/>
            <end position="864"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8178350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="994" mass="113178" checksum="8DB0AD3505A34ABE" modified="2022-10-12" version="1">MGAEDTHSEGENIMHQDSLFRDEEKMEESANVAYLLTLGILLGLTLREVNKKTKFPYSPMVLTCGLLIGLLQSSLGYLGESASILSHMHPHLIVYVFIPVLLFESAFNCDWYVFKYQMVNILLLAGPGVGWGAILLGIVFKLFLFYSDEDMNWYQAFTLGSVLSATDPVAVVALLKELGASAAFNHLIEGEALLNDGVAMVFFIFFNKLSKAAAGIGGGVTFGGVLLNFFRNSIVGPVLGVIVGFFGALWARRIVGDDVEVGWLTFVFTYLTFYWAEFCFFKTSGLLAVVSLGLFWSAFGKTRIRSESEHAVHTVWSFVQYSCDTTVFLLVGMIVGTEVIEERYIYTSDYFRMILFYFFMIICRYLMVISFWPLLKKYGYPMSQPELIVFVYGGLRGALGLTLSLMVGCDPDLPPRFRHLSVFFTAGMAALTNLVNGTTCKALVNYLNMIENPVIKKKVYKRYLTDMIVNQEDTIKELEGDEHYAMADWNQVKALVGSQEYLQEVVQLENEIKQIQGGNMSRISYEGLTDSETFGEVRYRVLRILKGLYYSKFEHGQCDEDSVRLLVESSDVALDHTGSILNIWEQLFQSFTSFSSIKYFFKAKEWTFLGGYAQQYITKHLGFVYDVTTTFISCASEVLTLQQHMPMNKGAVKLIMDEIRNEITKAEIYLGSLNDTFPEVIRAIQTKRASHTVLMHQRHHLEENQKNGLVDDKEFNSLKANIDARLVELENHSFDWQIPTFNAFAMEFPIFTGVPKEELDTIVKSTYEKRYQQDEIVYEKGMTCQNIYIVQKGTVDDEVGTHILKKGLGSLLSYANLIGDGKCMTTARAASETVLHSLNLNVLRSLMQKSEIFEQKIYINSIEYLIKLFESQAGPLAQMEAKRLGQFLRSKTQFKKFDVHSNVEFMFGGYIIKGEIQSCISDDGEGIERYRDYCYVPPRESDFTVNKPLICLVFDDAIDIIKQDRLEQFNLENHEEIVQERFSRIEIQNQQQEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VN72</accession>
    <name>A0A8S1VN72_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD domain, G-beta repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0670001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000067">
        <property type="protein sequence ID" value="CAD8177059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VN72"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="201"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8177059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="293" mass="34288" checksum="7CAA31BE40A9CFBE" modified="2022-10-12" version="1">MQGSFQLKEALADIFGQVKDIDEQIFGVLLEKLRKEKVQDCFEYLSDQINLGSQILQQQGNTSQTNSEQKLYVIVNEIKQITDILRKLKDHNFNSQDFSSEENEEFKQGLINRIQDNRMIIKFLQFLVNLTSIDNTLIQCVSNSLHMLVQMKVELKNQSLENIRIQNTSLIGDISGVKLNGAQLFNCKWRNIKIHELNKLLDDHSKCIRSVCISPDGITLASGSQDNSIRLWEIKNKYCSDYRFKEIQVKNTRYPFFNTPLKYIQYYGLPRLQLKWKLIQHILKYYEIELLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VPD0</accession>
    <name>A0A8S1VPD0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">60S acidic ribosomal protein P1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0670231</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000067">
        <property type="protein sequence ID" value="CAD8177482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VPD0"/>
    <dbReference type="OrthoDB" id="2194681at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05831">
        <property type="entry name" value="Ribosomal_P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696:SF10">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="70"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="75"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="95"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8177482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="122" mass="13118" checksum="E22202C3B4086DF0" modified="2022-10-12" version="1">MAKAAKDTRPIADQATSEAACTYAALILYEDNQDIDSTKLAKIIKASNLRVEPIWTKVFEKALKGKRIGDLLHGSSGSANSGPQAQTTSTPVVAETKKAEPVKEVKKVEEPEEDVDMGGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VQP9</accession>
    <name>A0A8S1VQP9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0710042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000071">
        <property type="protein sequence ID" value="CAD8179031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VQP9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8179031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="57" mass="6790" checksum="92A830B0C10454F2" modified="2022-10-12" version="1">MKVNLKKIESLKLSEFNFRIDIRECSFNFVFTDSIDKVEQNDNIVDIYVKTKINDQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VTG9</accession>
    <name>A0A8S1VTG9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 5A</fullName>
            <shortName evidence="4">eIF-5A</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0020091</name>
        <name evidence="8" type="ORF">PPENT_87.1.T0680105</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Translation factor that promotes translation elongation and termination, particularly upon ribosome stalling at specific amino acid sequence contexts. Binds between the exit (E) and peptidyl (P) site of the ribosome and promotes rescue of stalled ribosome: specifically required for efficient translation of polyproline-containing peptides as well as other motifs that stall the ribosome. Acts as ribosome quality control (RQC) cofactor by joining the RQC complex to facilitate peptidyl transfer during CAT tailing step.</text>
    </comment>
    <comment type="PTM">
        <text evidence="4">eIF-5A seems to be the only eukaryotic protein to have a hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eIF-5A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8132828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000068">
        <property type="protein sequence ID" value="CAD8177816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VTG9"/>
    <dbReference type="OrthoDB" id="436535at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045901">
        <property type="term" value="P:positive regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045905">
        <property type="term" value="P:positive regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04468">
        <property type="entry name" value="S1_eIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000080">
        <property type="entry name" value="Eukaryotic translation initiation factor 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000034">
        <property type="entry name" value="Eukaryotic translation initiation factor 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001884">
        <property type="entry name" value="IF5A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048670">
        <property type="entry name" value="IF5A-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020189">
        <property type="entry name" value="IF5A_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00037">
        <property type="entry name" value="eIF_5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11673">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 5A FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01287">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21485">
        <property type="entry name" value="IF5A-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003025">
        <property type="entry name" value="eIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01376">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0385">Hypusine</keyword>
    <keyword evidence="2 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation initiation factor 5A C-terminal" evidence="6">
        <location>
            <begin position="84"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8132828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8177816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="161" mass="17651" checksum="20E82B9E30CE467C" modified="2022-10-12" version="1">MSDDENQFEQGNAGSSHTYTESAGSLKKGGYVMLKGHPCKITDVSTSKAGKHGHAKASIVGKDIFTNKTYEDSAPTSHNIDVPFVTKKEYTLMDIQGDGFVILMNEDGSTKEDLKLPETEDDFNLVKEIREQFEAGKDLLIAVLSAMGEEKIVSCREAQDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VV71</accession>
    <name>A0A8S1VV71_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0760037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000076">
        <property type="protein sequence ID" value="CAD8181260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VV71"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="91"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="242"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8181260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="693" mass="83937" checksum="346FFFB205F71A08" modified="2022-10-12" version="1">MHEQYELYYTTFNQERERIRKFYTSLKSEESNRLLKSQSLNESLKKAEGFKNEKKLQIKQKAHKFNIKAKIIQRKNNQMQEHQFLGIIKQKNELVKKEIELEHRLNILNAEQTEQIRQNQIYKSMKQKIYLERVEQHQLKIEQEHQQLLEQMNNKFQKVEQYIQITRESEKQKFRSLSYKYLNYKQKAENLIQEKTENTFQEALDNMSKREQVYEKYIETKEKERKKIVRRNKLELVKFKRNKKRQESIEKSQKKLQEQERQKRILNKSTSIPKIIITEHDDQANNDVEVEVYEVPYSEEQTQTYEEKVITSKRNLKEEQSICTNINESSMQFTLPEPKIIESPNYKKPRRKSEKERQYQEIKIESEIRKREIRQRIEFILYQLANPYKLILINTYVIIRQILFGYFSSIMNKFELALQTQQELFNYILSTQWKDDSEKQQLGVIFQKLQTTCNIKLDIDEFFTKEKQEQLKSYLYEKQQQIRKINKNLQEILNKQFQGQQSPSCVIAQLPPRFKNNKESYLQQINYFKSNLIELKSCHSKLCQNIELKQQRETITKQISLLQIAENFKFELNCLESELMQNRLQLSEQFENIRKQIEIIIEDNLLQNLVSYCTAITKANILLKKQLIIVKQINSFETKDFYLQMMEIDKENKQTSYSQDTDITSQQSPQKNYDQHQIINKNIRNQECLCTIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W1G6</accession>
    <name>A0A8S1W1G6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cation-transporting ATPase</fullName>
            <ecNumber evidence="12">7.2.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPENT_87.1.T0820188</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.-" evidence="12"/>
    <dbReference type="EMBL" id="CAJJDO010000082">
        <property type="protein sequence ID" value="CAD8184198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W1G6"/>
    <dbReference type="OrthoDB" id="425043at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140358">
        <property type="term" value="F:P-type transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000023">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000068">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006544">
        <property type="entry name" value="P-type_TPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047819">
        <property type="entry name" value="P5A-ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01657">
        <property type="entry name" value="P-ATPase-V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630:SF8">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12409">
        <property type="entry name" value="P5-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="167"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="190"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="355"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="395"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="898"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="930"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="961"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1017"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1048"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1092"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="domain" description="P5B-type ATPase N-terminal" evidence="14">
        <location>
            <begin position="28"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="13">
        <location>
            <begin position="226"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF12409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8184198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1130" mass="129672" checksum="1306FD85E455C6CF" modified="2022-10-12" version="1">MKQPFIYHNYRVQFKHPINIQQEGLISEIISCRISLFKTILFTFLSIITCGLLYLVTRWDLRIFLFFRAKKCVPKSATHFLIIGQDKSQTLVKSNCSKDGNIFIEYRLYRYYFTENAFDPIETKYQTMIQQDIRQQSIDRNQQLDTFGYNNTEIPDKGILKTLIEEVLSPFYIFQFCSVLLWFWASYQRYATVILITSLISIFITLYEQRRSFYRLQQLSKFNIPVQIIDEGQVKEIESINLVPGDKLVIKDSMIMPCDAILLNGQVIFNEAMLTGESIPVLKTELPNNKEIYDPLDSGKQFTLFAGTTCMEIKGQEVIALVTQTAFNTQKGQLIRSIMFPVQNSFKFYADSMKFVGIMAILAVIGFIITVPNKIEYLLDGSISTWEFINEGLDLITITVPPALPTCLQIGISIALARLKNSKIFCISPQKVNISGKITIMCFDKTGTLTEEGLDMYGIRMIENQRFSKIVTSIDANTDVNFIKGMATCHGLSQVKGKLVGDPLELKMFESTNCELIEEQDGRIRIRNIDRINVEIVKRFEFSSKLQRMSVIIKDNGQYFAYMKGSPEKLRQLCNNQSIPFNFHQVLDFYALNGFRVLGMAQKQVQSIDLDRQEIESNLNFIGFIIMENKLKPITNKIIKQLKDSHIRSIMVTGDNVLTAISVARQCGLIENQRVYLGELSEKKYNGKYYVSWKDFEYNQNELNEDTLEPQQQIISNLNEIEKDIDVQQDFEYLNQRSFSKNLVKNKSLLEDPPPVTIQLDNISEIYTDGDFMEEQPWNENENYILAISGGAFMHLNKYGSQATLNNILERTIVYARMRPEEKANLILQLQKHKSQPLVGFCGDGANDCGALKTADAGISLSQAEASIAAPFTSQIQDISCVPILLSQGRAALTTSFCCFKFMALYSMIQFIQVTILYLKQSNLTDNQYLYNDLFTIFPLSMTMGLVQAAKINKYVPGSSLISFSVLGSVIGQTIIQLLFQLGVYLLLLKQSWFIPNNELNKEDLNDETMKICFENTTLFIFGNFQYLMIALVYSNGKPFRKPFYTNFYFIGSTIFLFILAIIFLLMRIEYIDEIMGFIFQSYDDQNIMPESWTILLGIIAIINAIITLIYEKMVVPKFLFKKKVHSIQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W4F9</accession>
    <name>A0A8S1W4F9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0810058</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000081">
        <property type="protein sequence ID" value="CAD8183557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035817903" evidence="2">
        <location>
            <begin position="18"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8183557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="360" mass="41514" checksum="36606F7D6248056E" modified="2022-10-12" version="1">MVHHKIVLFALICLVSATTVTNNQGVMDSYVNPGLQSSIDELTQSKIGHMVIDLAEVTMRVTGAVDSLVEGVKAFGRQIEERLNLENTQWEVVESEHNAKVISLSSQANQAEFDINREKKVLNSELIPRLHELDDILLGLKAKQTDNAQSFKEAEAARNSQHDSFVLTTTEFTDALRNIDEALDLLTDLFKSGEVRAAFIEITDEQKHKVNKALRQVKANTENFGHQYSSFIQALTNLTEGINFKDRQVLKEMCDFLNSLRLNIFDALTKAYKDEDSQKQQDELRRQQLTSEKXVSYYIYYSYILNIINKLQFKFLHLPKRFFIFIQIILLNFTYFQLYSIIIPQQPINFHFYKPDSYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W4T8</accession>
    <name>A0A8S1W4T8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0800217</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CGI121/TPRKB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W4T8"/>
    <dbReference type="OrthoDB" id="282986at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013926">
        <property type="entry name" value="CGI121/TPRKB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08617">
        <property type="entry name" value="CGI-121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8183362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="164" mass="19533" checksum="A2760F5C8AA3DFF3" modified="2022-10-12" version="1">MQQYFIDKDKSIWILLAQHIKDQQLFNKEIGLNENCIYALVNPEYLVSLQACLIAIYRMLKKKEYKLSSQFQRDLHYLLTPESQRNDVRFGITNILTQRMLCIFLTPVSEEQLKSFELDELTNLYQYCNIPQMIKYYKISDQELKVDKDVRGAVYNSIALKKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W8P4</accession>
    <name>A0A8S1W8P4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0870023</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000087">
        <property type="protein sequence ID" value="CAD8186354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W8P4"/>
    <dbReference type="OrthoDB" id="301104at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="342"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="154"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="344"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="360"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8186354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="379" mass="44466" checksum="458C64744422405C" modified="2022-10-12" version="1">MSQQFYQTYVPNPFIPTFNPTINSSRIDYPSTNSAKQVDIKESSNDQIQQNNVITSSFSLNPQTNKSIVLKPILTNQDEYQRKINDLEKSHKKELRDLEFKMQQQLDSLLQKRQQDDQFKQLTDVRQQIAELQSTLQGNTYGNPYSQQQQQQHLFYSQNELDRLEKQLKKKKNKIKLFKTEMDSLKSELQLRQQKITELEIKLHYSENKPIIQQTPLPKVITVPQSIPQPTTIIQDSPELIDRIRSLEFERDSLLQELRRFQSTFLPVQKQQQYAFPSVDEWNRLMHEYKVLENENNKLNTIILQKDDLIKSLEQRISVELTKQSALQAQGHQPRAISVLYDPQAQQQSSKSQQLYPIAVQNTEQQSNIEPTLQNPNAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W9D1</accession>
    <name>A0A8S1W9D1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD domain, G-beta repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0880007</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8186838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W9D1"/>
    <dbReference type="OrthoDB" id="5594999at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="264"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="312"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8186838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="551" mass="64647" checksum="B8CF5ABC62F27B2B" modified="2022-10-12" version="1">MIQQKQLDYVTNFSCSQHIGQPINTILLDQSLLIEKRLLCSYCVENIDQNLKTIEIEKALEITEQKVREKSRNDLDFVQSQIINLHKLQSNACQMRSYINSQLESIIQISSDWIKELKTFNNQYQMQIMIKELDNLLNYQNKETERDSKLTITKLHQIAVLQSYQMSIYLEQFSSFQEYQECQQILEMMTNIDKIRIQHSIQEASKTKIQPFTFEIIAQNSIKQPEYCQAIAINQDNTLVLVGCEKEIRVFELIQNKLVQIDILKGHNDSIFTLDFFKKQKNSFISGSRDDSIMIWLYQNEMKKSWSCSQQLVGHTSSILCLIYNQKEDLIISGSDDKTIKFWSYNQNCENNNKQLWSCSQTIRKHSSYVYALSLNEDQTKLISCGLDKLILVMELLKTQYSYSWIVLQEINVENDGYRLCFIENNIFAFQPRSYSKMDIYEITGNSEGQFLKTKEIDVKGYSQSCDNLFPSKFSIQKCILMIKNGKNVNLIRCGNYCNGNIVSERQFFLEQVIEFDDNCIYGTLSDDGQYLLTWDYFTKEIQIRCFQSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WA68</accession>
    <name>A0A8S1WA68_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0880059</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8186924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WA68"/>
    <dbReference type="OrthoDB" id="307080at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044661">
        <property type="entry name" value="MED15a/b/c-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33137">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 15A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33137:SF4">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 15A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="411"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="308"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="330"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="356"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="411"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8186924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="646" mass="76612" checksum="CEED0BB3FD867343" modified="2022-10-12" version="1">MNNNSNTKLDILPKLMTSHHKLLEPAYLKNTQFKMSQNESKLLPVIQRSSMMQPNSSSKQLIVQRLIGNLEFSSRHFGAACDRGFQGLSKYHYSQKFGHSAVRLEPLNRNIIAELTDAEDIDEIPKDERTYYKRIYKFKNMPILALIKSFEIEWVREFEDEVNKKSTKSLQLSFQTALAIFNTFPDPLEFFQKNLQLLERSSVALKQKGLTQLKYKVKNFEEEEFQRMIVVKNQSGQGYFRIYFPTIQLYLQEYSHNQTIDLSPLRLYEIVKNNFFEMASIVEQFDLEKFKIYIQDSENKGILEQLDDQTKKSDENILKPEQKSNENILKPEQPESQEIEQQSQKQINDQGQSVDQQQNTEQQQQQEQKEQQQQQQQQQQQQQQQEQKEQQLQQQESTQQQQQESTQQQQQESTQQQQESTQQQQETPKQQQQETPKQQQQETPKQQEQVLITKPVNTDITIIIIEEKHEFPEQILQKKWIKKVVPKDNIYYTVDFLPFQILIREKRSQKIIKMYSPSLKELDFILFQLNHKNLLQILEDHVKTTFDMPPEAFDYKDFKKGTKITFLLDEITEKPLEITEEQLNNGLLSSQTKEIEVNNKDLQIIIEPCMLEIYNQLKCSTIKRPCTSKELTHILEKRYSIGYRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WGM2</accession>
    <name>A0A8S1WGM2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0900067</name>
        <name evidence="3" type="ORF">PPENT_87.1.T0900080</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8187835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8187861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WGM2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8187835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8187861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="239" mass="28081" checksum="25C5E6067F4BEBAC" modified="2022-10-12" version="1">MNLVSLYSHNTKILVFCFKSGAIGFNTQILKINRPQNQLEKRMNLKGVLFLEKIVILLSVFYFYFNLQQIYKNKDQFAPNFNPILECQNNCYLDFSFVISISPISQTKYQQFFFERKTDYLLSTILRAQNPNILSYLDSVLQKSRNLCGHRPSDPYTRFSISQVYTGIIKTDRDVRNISSSPSILFLKNQWFNNQHQKAKRNIHLQKKVNKFNFNQSPAKIGLVPQRDNNEVESQKVQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WQW0</accession>
    <name>A0A8S1WQW0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0980112</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000098">
        <property type="protein sequence ID" value="CAD8191160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WQW0"/>
    <dbReference type="OrthoDB" id="289584at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8191160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="170" mass="20032" checksum="D0EE3C81C757C06E" modified="2022-10-12" version="1">MSNIFFEPDDLFNPSTLNNLDEKSQYEVCDEQQEGEKLYYSSNSIQNQKAQNKNYQEAKNIPKNMGVLIKNYFLNNYKDLVRQNITIKKFISKVNNKKNYTRKDLKVLFSNEEARFICKEYLSSFQIIKDILKSTKISDSEVILKYVKKLFIGTQDPQSLSSLKYSNQEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WWD9</accession>
    <name>A0A8S1WWD9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1050025</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000105">
        <property type="protein sequence ID" value="CAD8193752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WWD9"/>
    <dbReference type="OrthoDB" id="301067at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8193752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="545" mass="64695" checksum="52A41584D66371F5" modified="2022-10-12" version="1">MYRFKLCLSYSRLDQSLSAFLKKYKTNQKNVIQVDNKLLEFDKSTQRQPKPIQPQLTIPQDDQLKQKYLLQRVKVIDLNEKDNYSHFNRAQQLALDKQVKQLGEDYKVEAEVLQLQSKGELLIDDTKLPKQFLATLDQERKKGKRMLRRFFVNGEHRYMKENIYKFRNVAEWKKYMRFKKPMLIDLKMEQFKRYPGTDQGKYPIQDKPGFTKWFRENHTQKTVRAIEYLVRKGYGKLPEAFTNKRGHKSKSKRVLAKIELEERLERLKQEVHEESNWETVAPGPALFVQETMMEQLLKRNPIKDLGLNTHIKIQTHIPEGEVQPLPEKFPLEKTELIYYLERWSFFRSFGAYELLKDCVTGKLQQQDSVPVKSGSLNTYYQTLPKHIRENPNVKNVYRGLEFANNQMTLAEKENSLNYAARLSCQFDELNQEVYDQGSSGYRPMVSRFDEERLMQDQENEHVDTAINPDVVFKYLEQRAHTPRHVAKDDVPLPDWAVDKSERVDDIPVDYYINDDGFWDDYIKAKEEKHLKDSPINGGKKYFRHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WWY2</accession>
    <name>A0A8S1WWY2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1030106</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000103">
        <property type="protein sequence ID" value="CAD8193100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WWY2"/>
    <dbReference type="OrthoDB" id="285613at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:004958">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24073">
        <property type="entry name" value="DRAB5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8193100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="307" mass="36755" checksum="245D999FB3D748DC" modified="2022-10-12" version="1">MNIESQDFRYRDNKNYQSLSKLQILVVGDGQTGKSSLVNILTYRKYPDKMQGKVCKQPYKTQGCDIRMALRNDFTQKQQFLLQILDLSGDKAQRQYLDVYISRLKLNGIIFCFDVTNLKTLDNLRKWIQRLTIKSVIHEENEEVLETDNSEFSRQSSIAIQDEQDCLLNYNSYKGIPMDEILQIPFIFVGCKMDQIPMDKQVRLRQQISERIQQVYKRSNTVILLSTIKPVTYSDQYKQLEECILRIKQKDKLEEFFIREKIQSKMMKLDESFWKSTIQTPFEQKYQLFCVYMKHFWQRLMCKKRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X3D1</accession>
    <name>A0A8S1X3D1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KHDC4/BBP-like KH-domain type I domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1090011</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000109">
        <property type="protein sequence ID" value="CAD8195240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X3D1"/>
    <dbReference type="OrthoDB" id="5989967at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22386">
        <property type="entry name" value="KH-I_KHDC4_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000070">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055256">
        <property type="entry name" value="KH_1_KHDC4/BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047889">
        <property type="entry name" value="KHDC4_KH-I_second"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031121">
        <property type="entry name" value="RIK/BLOM7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15744">
        <property type="entry name" value="BLOM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15744:SF0">
        <property type="entry name" value="KH HOMOLOGY DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22675">
        <property type="entry name" value="KH-I_KHDC4-BBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KHDC4/BBP-like KH-domain type I" evidence="1">
        <location>
            <begin position="169"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8195240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="323" mass="37787" checksum="202F114171D7F424" modified="2022-10-12" version="1">MSFQEISQQIEDMTNFLLEQSDDSLVNASPTNREQINLIIHDQKVKQRISAMLKQKSRQYPHQYLTVELSSEDNYEIELDQDDIKQVCENFGEVKQVYTKVNGAIIIKFNSVFDAFIVYKLLNKHQIKELDVTIKIEFTSQQDLLEIMELQDSNQQKFTCRYDVQIDNDKDFQVARKIIGAKGCNMKKIIDQCLFDCDTKEQDLVKLRLRGRGSGYKEGPEKRESQEPLHLCVSSKHNHLFLRACQLVEQLLNKIYDEYKMFGLSKGKKQLNYGIKKVQQPQKSVIKPNFMPELPNFNFSNNQFIDFNNENMMGYSNLPNQLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X3T1</accession>
    <name>A0A8S1X3T1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1100043</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000110">
        <property type="protein sequence ID" value="CAD8195627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X3T1"/>
    <dbReference type="OrthoDB" id="428832at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051826">
        <property type="entry name" value="E3_ubiquitin-ligase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765:SF434">
        <property type="entry name" value="GB|AAD18119.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765">
        <property type="entry name" value="RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="9"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="115"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="164"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="302"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="365"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8195627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="412" mass="48302" checksum="736BA53E275E23A3" modified="2022-10-12" version="1">MYCDSISDGLVALGVFDGSLGIVSLVLTIMLEGTQEKIYTIMCTITEFWWIWLIVSIIYKKLSVGVVCSVKEVLMLPDYQFSRYVAAILSLLQFTMTICLLNSDDIKRFTGEMLALAMLVPVFCFFKGIALIFVLIQQIFLIFTGKEKNQYFTLIQDQQSKKQVIFLGIVFLIMTSLYYFLKSLFVFTIYNNFDDLNTKIFTIMIIIQSLFLIIFSSFMTCQLITILKQIPVNEDPHLMNPNQKCYQNWFILIKSIYFSDKMKCLRRITYFFWCIQCIYGLIQFPFFKLIQITQQQLYTQTSFMYFDTFLLQGLIVTQIILQMAKKFHQIQDDEDPFLFNIKFSNEDSSKVEKKQKIIDKSQLQCGICLEDLKVGQIRQKLTCHETHIFHLLCIQKWTQTQNICPICRTPCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X486</accession>
    <name>A0A8S1X486_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Essential protein Yae1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1090107</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000109">
        <property type="protein sequence ID" value="CAD8195415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X486"/>
    <dbReference type="OrthoDB" id="293330at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8195415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="13598" checksum="7E196D31337D3260" modified="2022-10-12" version="1">MNNDDQDDWYDSNLNEKEIERQIAQKDEQIMINKLENEGYREGLVRTTEEEEQNLINKGFNDGFIAAKHQFKVEQTIDLVLQNSKLFSQNIVFKCEQLKQLQNYDQVDLDQLLGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X795</accession>
    <name>A0A8S1X795_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1130104</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000113">
        <property type="protein sequence ID" value="CAD8196709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X795"/>
    <dbReference type="OrthoDB" id="301459at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031464">
        <property type="term" value="C:Cul4A-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14255">
        <property type="entry name" value="CEREBLON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14255:SF3">
        <property type="entry name" value="SULFITE EXPORTER TAUE_SAFE FAMILY PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="280"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="331"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="360"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="403"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="432"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8196709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="478" mass="53485" checksum="DD38001762D5DBF5" modified="2022-10-12" version="1">MPGNNLCPSKELYSYNCTTLSHQNLTTNCGPFSSCKQIFSSGQFGCCRIGYLAANAAQIIVYIIILPIIGIGILGALGGGVVKRPFLEATLNFDSESAGNMTMCLQFGSQLVNIIIIFFQRHPEDPDRPIINFEIALIYCLAIPLSQALGTEFANYLPLGSLLWIQNIFFVAICPILFLFATKELTLQTQKEQDNEILLKSALVPLEGLKGQQQLQDQEAKLYKKFYDDQCNKFPLPAILITFGNFAINEAIILTRTTPGLTSPYFNDPKKNNKGECEAWNFYMLLVLFAVNTVITLVVYFIKRKEEYTKDAVKFFNCERFYTPGTRFWKIYAAGWVTGIVAGFFGMAAGLIMVTTMAEFGLISAVAGGTANYCYFIICFQLFATVLSNQKEENNFGVGDQFFFYGLGMISVLIFTNLGYYYLKKYNIGHALFYIDFALVLLNIAGNLAWGIETSDRHNYDYLMKPPLSCSTSQKGDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X8V6</accession>
    <name>A0A8S1X8V6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1150083</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000115">
        <property type="protein sequence ID" value="CAD8197392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X8V6"/>
    <dbReference type="OrthoDB" id="308067at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8197392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="679" mass="81366" checksum="5A1FA2F0B1004175" modified="2022-10-12" version="1">MNQQPFTIQNSIQYLQQYQGLLNYTQIKLTDENNNRYKYPVLTLNCDHTRFALNLETVLQSINQQGYFTCSCNNITIKHPRQLQKDIRWNSNEEINEIFDFGVVIHNYLFHQFLRAKKFDQVLQQNFVLQQIKILMDSQNSINISQILQRISKNNQNQIIFKVICLLDFVQINFPIRFIQCQHPECYDLASLLIYQQENSNNNSQNDQLKQFKCKQRQCKVKVDISTQEKIFQNIYLDKELLKLIKKELPTIYKYIYNPITLKIQQDDVIVDPQIDQIQKQEFKQQFSASDKDYQDYQKQVNTIAYTILQRTPGVQIEQNLRLHRYKLFQVLVMDQNIEQPARCVNCPISKVMNLKSIISNFQSQKRKYKQNIRPIKCVLCEKEYSFNVNISKIVYFDTNLFNAQEQGLLENVDQIIRYNGKKFMKEEFMSKQKLDLSDFKQYLQQSFQCQRFTFFNLYCNYNSRNIIKLPLILKNCPEQRIIEFESFYNRIEQIQTNDYEQKCLQFCDCYYCSNNRFSFKEFPFNVYFHEAFYLALIDFNNQNRNQNILTYDFEFNCIIEKQNNLQIPQESIPIDKQYFKQGFVSLLDDEQYQELFQIKGIKGNFQYQIVQSQIEVGLATLTNTQEGYEWSTKKEMINQKMKNNTIANKWGFEIIKTETQFVNNEQKILKGKVSSEQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XA67</accession>
    <name>A0A8S1XA67_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Insulin-like growth factor binding protein, N-terminal</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1170032</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000117">
        <property type="protein sequence ID" value="CAD8197977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XA67"/>
    <dbReference type="OrthoDB" id="282383at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8197977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="546" mass="63084" checksum="397C35840893860F" modified="2022-10-12" version="1">MIVLQIVKDGQCNGIQLFGGYEVFGKNTIITKLISPPPHYAIMITLEFWKIDNWDNEQFYIYVDQNLIFSQIYGESNTNSNLCGQPLGSSWSEEVQQISVTKNHNYKTLFILMTSNLQGIRDEWWGIRNFKLYYYPCPPGCQTCTSEDSAIDCLIWIVQYQSLLDVLYGQFQMDGWMILEGFQNKTSCSILPLLGGFSKTGQKSKIIKSINLPVHCFVKIKFSFVFIDKWENEYAQFYIDDQLIWYYSHKSVAEEITICGNSFGEKILNQEIILPHKQQFLQMQFTTTLKNSPSQQSFGIRDIQIFINCPFGFPFDFSCEGICGNGEIDRAEQCDDGNIQPFDGCFNCQYSCIQGCSNCINGICYECQEGWIFYEFTCTEDYFINNQDQYDDEQQQQCLNNCKLCIYGVCKECDIGYYLINNQCKTICGDGIIEMCELEQQECLNCQLNCTKNCDVCIKGKCHQCIIGYTFDYIEQYCQPICGNGVVSNDEECDDFNIENGDGCSSQCKIEQNYICKNYQYSFSECSYEKYPSFQLSLIIRLLFLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XBY0</accession>
    <name>A0A8S1XBY0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Inosine/uridine-preferring nucleoside hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1190170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000119">
        <property type="protein sequence ID" value="CAD8198776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XBY0"/>
    <dbReference type="OrthoDB" id="432381at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016799">
        <property type="term" value="F:hydrolase activity, hydrolyzing N-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001910">
        <property type="entry name" value="Inosine/uridine_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052775">
        <property type="entry name" value="IUN_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46190:SF1">
        <property type="entry name" value="SI:CH211-201H21.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46190">
        <property type="entry name" value="SI:CH211-201H21.5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01156">
        <property type="entry name" value="IU_nuc_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inosine/uridine-preferring nucleoside hydrolase" evidence="1">
        <location>
            <begin position="30"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8198776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="350" mass="39223" checksum="5A3D19C24D3F53BA" modified="2022-10-12" version="1">MAEQLKFTHPFPKLLFDDSHIQFKKYSKYIIDTDAGSDDAHAILIASYILKYIRTDAELIGITAVAGNAALENVIKNVYITTRIGHFGENPPKIYKGCRTDTLRRFYRDNYFLEDGLGGQQLRLLTELGLQDKPLEYFHEKQHACDFIKDSIYKYGEDLCIICIGPMTNIYLTLQMYPDIVDKLGCLFAMGGTYMGVGNAANSVAEFNVQTDVEATAAVAMAKFKQKMLLPFDVVLAYTLDKQNGKVIFENGAKTKKSQFVESIFKAVSHDGNYILCDELAVMVALVPSLIIYSLQKNISIVSDGQARGQVIIDWLTLMRGNQKSVVSILTAFEWDSVIEIATKANQDQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XE94</accession>
    <name>A0A8S1XE94_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1210090</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000121">
        <property type="protein sequence ID" value="CAD8199259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XE94"/>
    <dbReference type="OrthoDB" id="310405at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38019">
        <property type="entry name" value="KDA ANTIGEN P200, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38019:SF1">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="170"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="549"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="612"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="682"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="549"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="565"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="612"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8199259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="723" mass="88123" checksum="FBCC544E6E052AB2" modified="2022-10-12" version="1">MKVSLQDFGFHQPNAPEINTPRSLEACRREGIQPNEIVKIPFEEFQKRYKMKNLDHKGIEQFYKHYEERREKKFEELIKSRHIVIEDEKTGVWSSEGLSKSKVISKYGQQETSSLVEKEKQQLEKIKKKQQKEIEGMLEYELKMQEIRLENEKKQAEEIAKQQQRDLEIQRKRAEQEEQRRQKELEKQQKREQEEQIQRQKLKDMELREKERLKLEELKQKEREEEAKKKDEERKAIQEKLKKQNEENQKIQQELLEKRKQEMELKAEQRRKALEEQKLKKKQEAELARLQNEERIQQAKQRNEAELLKLKDDFERKIKLSEIKQKQYEEEKKKKFEQQRKEAEKHSELFQKVIEQNNQMEQEKKNLYLKKIQEAEERRKQIEEELEKEKELKRQLEKEKEQQRKQVLLLNEENNKQKIEELVKKINEKEDILNKIQEERNEQLQDKKTMDILKRFDKRENVERIMRKQEYDKQKLQEKIVEKMQRADKLSEDLDQLLSQRHNLRREIEKQKREIMIKFEKVREGKLPPSEIQKQLGMTETDLKFTTNQTNPYRTFSSTRPKTNQVKERPDKIQSVRPNKIQSAKPEQIQIKEKIDIKPTSPIIEQQIHKTTPKFHKKIDAKKKSEPIRAEPPSPQILIQLNKMVQKHNQDMMRLLMEEHANENLRDEKLNQAKGEEKAKLEKEYALERVKAQLKIEKLKSNQEEEQKQYKLTHNIFENDIIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XHA1</accession>
    <name>A0A8S1XHA1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1240132</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000124">
        <property type="protein sequence ID" value="CAD8200433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XHA1"/>
    <dbReference type="OrthoDB" id="1717591at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23172">
        <property type="entry name" value="AUXILIN/CYCLIN G-ASSOCIATED KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23172:SF101">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_124, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="118"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="178"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="267"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="118"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="153"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="198"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8200433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="431" mass="50958" checksum="6CBA50948C7F8045" modified="2022-10-12" version="1">MKQIPIVYYYQNEPKTQFMFFFQGEELIVKNILQQFPFKITTHHIRFKIFVQDIEPKTYAWFDISNPNAKVPVVNDTVFVKILTLQHLSYSRIAKKMVESLFQDQKVGDLKIQQNPIQQSYQTQKQQSNSSPKLQQQQNQKQIQQQGGERYSNKQFSNLSNGTSQGGDLLEAFEKFDYKPNPHHTQSQPISHTDLDINNTNLNLNQPKQQQNNKDSLQFLGFNPQSQQPQAKQGEDLFEFQTNIDKQPIVQKEQSQREDLFQFDVPKQSIQQQQQQQQQQQQQQQQQQQQQQQQQQQYEGLTDKQIVDLRVEQAAEGLQKIWQEEEDYNVLRQQAKDEVEPKILNWAYKNNVRNNLRLLLSTLNDVLWEGTDWQCSIGDLMTDGKMKLKYRQALLIVHPDKHNQSPPVQRYIAERVFYELNQAWNDVKNKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XN26</accession>
    <name>A0A8S1XN26_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1320031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000132">
        <property type="protein sequence ID" value="CAD8202760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XN26"/>
    <dbReference type="OrthoDB" id="298084at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF122">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN SKYWALKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="1">
        <location>
            <begin position="240"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8202760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="410" mass="48228" checksum="01710D98F1C704C7" modified="2022-10-12" version="1">MEKCAKHQDDIKYIILEQNSVKLACPECRDEKLEQGLQLEKFILIQKVLKLPEVLLSKIDIDMPFKNFFSNIVKITNEDLNNFKIEWIKQMSDIKDSIEKLINETNNYFEILSNLLSSYRIELKKIIKFEIFEQYIQANINNENFNQQTSAIIQKTRHEINTQIELKIQEYINQMIKGNNDEIKKNIEEQIKDYKNKVINLKIPSSKEILDQLKSGIEGFQKAISEKSHQVNPFYIKCSNLLTNTNFQDILFRIKQQQNPNNQIIYQGSKDGFQTTTFWNKIQNKSNLLMIMKTKNSQCTFGGYSPCQWIKSPQPQFMSDDQAISFLFIQFPNLQLQIYPIKNDCKHEAIFVNQNLGPQFGKNDLQLFAEFKEGNINIGQHYFRDPKENLQTFILGPKLTEIMECEIFQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XPK0</accession>
    <name>A0A8S1XPK0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1330089</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000133">
        <property type="protein sequence ID" value="CAD8203221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XPK0"/>
    <dbReference type="OrthoDB" id="336906at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF519">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE MPK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="5">
        <location>
            <begin position="8"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Dual specificity phosphatase catalytic" evidence="6">
        <location>
            <begin position="160"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8203221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="304" mass="35926" checksum="FBC151159898648F" modified="2022-10-12" version="1">MKAIQMMEFYNMMNDNRHLLIADLRKLEFFEQKQIRGSFCIQNDVSMMTSHIEKHDTNAKKNEEKYHTQPIRRMVIVNDDYQKIEQEAQLIKDLIQSFKMNEYKIYYLKTSVDAFLDKYPFYTFSKTILQNYENLDNNEDQQKVLFAYSQFPQQLQENKLYLGSNLNANSKKQLDALQIKTIIDLKKYEPDAQLSSWKKEQYNYINYPISEGGEQFIDFYQITDLIESQDAPILLCCPDGYSITSVVAIGYLMTLKKQNFNISSLKVFQIRGNTTVNKQLYSQILSYDPKTIVNKIIQQQLVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XSW8</accession>
    <name>A0A8S1XSW8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1360003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000136">
        <property type="protein sequence ID" value="CAD8204073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="317423at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2319"/>
            <end position="2337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2378"/>
            <end position="2399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2430"/>
            <end position="2456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2468"/>
            <end position="2489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2514"/>
            <end position="2540"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2651"/>
            <end position="2671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8204073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2772" mass="323091" checksum="EB81B3FCFDDFFF22" modified="2022-10-12" version="1">MFKGLLSYYLLFAFEISNIYCLRTMNLQYPVYTFSIQNNQIEMENDLCGIFGVWSRYMPLSNIVQVGNIGILDSNCFHLLNAQEQTTKRINFLHYECLDFETKQIHKYVQFIDNSGIDHIIKIPIDDTFYENVWYYIEFRLYPTEKLFKIHLQQQGQQPLSQNYQIEKPFYDQYLIVIVGGDLKIQNNQQLYTSQNENLSYFPGKIRVQDFYQSYVLEYCEDYNEFIEENYGACECRISYKIPINDQIISKQDKLFFISENINCETFLLSGWIKINDIHTYVKEFDYKFLKLFGNFMKPQLTDDNLAAFQLTYRISSNGNQIIVSTYSYTFPTVNIDFSKNSFLIEEVFNFASNIKLWHYLVVRKYKTNINIQITFYEGYRQEKFEFNKEVKQFNLLQFKLLYGNLFQLSSNYLKLQIQGFQFQNCPDFQEQPIDCHATCKECDGPTQNDCLSCFESSNRIYLPEFKQCLCKYGMIDQGYICITQQSLNLTTTSDQFTYNGCKYGYYEMDGICEKCPSIISKNLVTCLECLQNTKEWLSTGCCEIIIYSDNEGNTLKQFEEFGCHFLSGNKVLDEDICLSCDIMEPDIKYPYPIPRLAYCYQPYYRNFEKKCILCDIKYCVYCFQYFESDQTKTTLDQVFDYDIVGEEIKTGCAQCIDGFMYSFEKEQCIYQNPSIPNCLRAYVTKSGSEICTLSSIDDFSVAPEIINCQHLILNCKHCIQTPEMIIRCVICEDGYKASAITGICTKCSFLENSKVCVEWNQYNLEPWKWFIQSFRIKFLNLQNFYNVGILRIPVEYVLECLDGYKVINNECKKYCNDNCLVCKANTLKSYFYCEKCGLNKYKQPIRFQNNGICIACSSLCQACEERSTEEIQNINSQFVRTSESSIYTLKCLQKVPLPNITIDPILSIAYYCFNEDCDRLLEYTVLYFFFLQVLSQIFDYIEAYFNYEYFNEIGLQQIALILKMNSEYADRYYPTFSNDLKEKVFSLQLVQVRLEGDLQPINFSPSFKILNCDEITIKQIVYNIQTKLELTFQNREQSISVNIIDISFYSDQANPIQLQIIEDNNLNYNLQNVLIYDANIYNSSIFSVTQTDLEGIINITNLTLSNCSFQNSTLLFFSNFNKKIIIDNLTINSCQFYNSSIIRFNFTSFYFADIIIKNVNIQNSLFQDSIFLYNNANSSVKINQFLLLKSQINGSRFISFSKDFQFNNALLKENLLKDVQLLLQLSLSFLNSKIMIDNFKILKNEFFNFQIFNTELNQVMNQVHSTLSYFQFEDNLPDPQENQLYLFIITCQHLLISNVLLKNTQNFRFFSFIAIPTIRIENIIYENQYQSEKVPFTIECLQNSATNSQLLQVQGFSNIMLQYIQIKNQISIDKSIISIQSNPLVDFNQIEQILFREVTFIGNILLKINLGIFFSLFEIYSEKTQKITIENIIFQENIFHSYEADPSDNSASLAHIESGESKIRISNILCSNNTLTNSSNSFISIISDEISVEKFQTFNHNYLDVKLWQKYYQLQIHSQFNQNQIIYLIKNTYKIDTIGGGLSITASQIQFNNGLFNNIIASSSLIFKINLKGNGIVIIKNCDIAYAQAQLTSTTQVNGAMTIDARRSLLIIQIENLNLKYVQNKLSSSIFSITPSLIKNKITLKQIYALNCFSLLNQLLHLEFDYKNAENNIVIIENLHILQTEAAFMLYVQSVGKLNRIDIEKAIQDNAILNMIGCQAYIKQVLIEGVLSSSILKIVDYAKIVLTNFQFQYISTFYPLHLIDLSQTNVLQSDLMLNNFSIQNGNSFQFNKYQLNYYQNNLNVDQQIKQCTLISDYVTDQSSDSESIHIFFEEMIQNSNQNGSLIRINSITNQTPITVKKILIQNNNCEHCLNGLLYFELVDFKIIKIQQIQCVLNFIKRNGCILAISKNSLDRKFIIDQSTFISNQGTQGTGVQGQNIRVVLANSRIINNVASQKGGGIYFNPILSSFLLYQTVICQNSAQIGGGVYLEGNSSLSNYNFIQSLMIQNSAQVSSNNLYEVPSHLALQINYQVMHSLKQEIDNQIIYLLQLHPYKIISQNQVITTNYLTIPSGQMLANYELYNPKLRKYISYLKEISVIFKNSRNEKQHSIINSTCNLTQEIYDIQSQIALDSLQLYSIGYNSDTNKFDIGSTKFNFDPYQQESKKYEILVECKTDYQYKILSYKMRVNSFFCQLGEFYISEGCQACQSEQGFYSVTYNSTKCSIFDKTKFEAITTNQILLKPGYWRPNYISDYVELCYKNIYQCKGGWSVGDDICNKGHIGGLCEECDNFNIKGDGQFFKNQQQLDCQQCQELSKRVIAFLLILIWALLSTLLTIRSIEKSNLLFRYLKLKLKFADILFNLDQDHESILLKLYLNYLWIFSLIFTFNINFSFSLSFLKQSSDTTYFMANFFECFLSEIEGIELIYSRIILTVGLIISQVLIIQFGFALFSFFTAQKYKSRIMSNTILYLYIQNYATLIKQFFSTLAIRKISQIDYIQGDVSLLYGSNNHFNWIYAFIIPGSALFGLIIPLSLYIFLYLKKNDLNKIKYRSHIGYLFNEYTRKNYFWEWIKLWNKTIIIIILIYFETDISLKASSLGLCLLIYQYLSQHFKPYNLQKFNLLDIQTGQLCSSAIFFAAVKYICDQQENYTLSSLIQTIIILISLILSYPFIRGILKVYFKKYKPGVFEIMLTICKKCYPNSKFTKYLSLRLIILRQREKKIKSHFQKMKQAFKKRKQNEKKQQKIVLNSNLSKNNTMNLLLIQSQKKEFDNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XU06</accession>
    <name>A0A8S1XU06_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Acyl carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T1380023</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Carrier of the growing fatty acid chain in fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl carrier protein (ACP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000138">
        <property type="protein sequence ID" value="CAD8204650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XU06"/>
    <dbReference type="OrthoDB" id="448946at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000035">
        <property type="term" value="F:acyl binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000036">
        <property type="term" value="F:acyl carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003231">
        <property type="entry name" value="ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863">
        <property type="entry name" value="ACYL CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863:SF28">
        <property type="entry name" value="ACYL CARRIER PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="11 12" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 12" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 12" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Carrier" evidence="13">
        <location>
            <begin position="55"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8204650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="142" mass="16281" checksum="EC81A774E3FEFFDD" modified="2022-10-12" version="1">MIRGLRLLQMRPIFCFAADKTPATTQGKQQQAKPIQVYKDGQLITKNYVQLKKQQDIEGYVLTLVKNYYRTTNKSALTLDSELEQHGLDSLDSIELSMQIEEDLGYVISAETLPVLNKVRHYVNYIKQVEQYKVENNSAPLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XW16</accession>
    <name>A0A8S1XW16_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1410086</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000141">
        <property type="protein sequence ID" value="CAD8205651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XW16"/>
    <dbReference type="OrthoDB" id="10336580at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8205651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="41" mass="4959" checksum="8ADE942617064CDA" modified="2022-10-12" version="1">MISYEVISSKIQFNSWNSMFDAIILVFDEKYLNIWESHEMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XY84</accession>
    <name>A0A8S1XY84_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Choline kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1430160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XY84"/>
    <dbReference type="OrthoDB" id="3649325at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004103">
        <property type="term" value="F:choline kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004305">
        <property type="term" value="F:ethanolamine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14021">
        <property type="entry name" value="ChoK-like_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603">
        <property type="entry name" value="CHOLINE/ETHANOALAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603:SF93">
        <property type="entry name" value="RE24176P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01633">
        <property type="entry name" value="Choline_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="288"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8206356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="401" mass="47400" checksum="66F32A2B11200CB6" modified="2022-10-12" version="1">MNSRSTHLTEYAIKEIISEKFASWKSVSPSNISITRLTGLTNITYKAQAMIDTTPQTILFREFGNAEGFVDSQQERIIFKTISDMDLGPQELECGNSWRMEEFVKDGVHPNNQKMAEANFQFKSMGVLQKFHQMEIPIPNNGSSSIINKTLSGEMKANVIKKIEKRELYTDVELKQLECMENFINNEEEFNYLNKMTRKENNEELKFCHNDLNQLNIFNTSNKDKEIIFIDYEYCSYNYPSYDIANFLNESAINYQYEEAPYYILVDDNFNTAPIQAHFLALSYILNKECTQIEIEQIQKLINEKTNKNKDELKTFIELVKQILEQRLSEQEIVELFHAISYLKRRIRRQQILSNLNWIWWSILLAHEKNSLNFEYIDYGFLRFKMLEKLLELEKARKNTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XZH6</accession>
    <name>A0A8S1XZH6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1450080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000145">
        <property type="protein sequence ID" value="CAD8206836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XZH6"/>
    <dbReference type="OrthoDB" id="70770at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8206836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="353" mass="40912" checksum="01127D67634A3752" modified="2022-10-12" version="1">MGNASTCCSHPIVQNKDALIPYQKNMLVNEDCDVHEWEADDFVKIGLQSHPVKEHKLFDQPSVIRRMESVEAPKLGQRLEAFPEIENQIVRTVLSQLPPLNIPQDMDRGEENPPIKFDNDFIYHGEWKDSQKHGYGKLLWPDGSYYEGGFVNNETSGFGRLIHSFGDYYEGIWKHDQANGYGKYHRYRNQATYEGNWVNDKQQGQGKETWQDGSSYEGEYFNGKKQGRGMFKWGNGNMYIGDFKNNKMDGHGVYYWKSGKVYDGEWKENRMDGEGSFNWPDGRKYKGGYKNDLKEGYGVFEWSDGRYYKGEWKQGKQHGKGVLRMDQSKEITAEWVNGKMLTDKQMEQQATGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y1D8</accession>
    <name>A0A8S1Y1D8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1480013</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000148">
        <property type="protein sequence ID" value="CAD8207593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y1D8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="108"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8207593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="333" mass="39051" checksum="ADB70B38418D6353" modified="2022-10-12" version="1">MQDIQEVNQKSFYYELKNGDPILQSQWCTAMAIDQNNEMVLMQQNGLIKCFQFKHNVLKILAIIQNPKEIVKVVMFLKNRSTFLSTKGDSIINWPMNLMQCCKFINKQKLHSNIINCLILNQCQNLMVSCSGDQTIKFWQQQSNLWFCQQTIIKHKQNVKGIAINQKGDQVISYGDDMYIIVMKFNYQEQIWQVQQKIKGEKRLLGLCIISDNIFTFQYELSKQLCIYTNSIKNLHFRKTQEIPITLGKYFSYSLSKFIPQKNILINFSGSQVNLLKINQVNQNNKLIYKLIQIIDFQSINSGIISEDGQYLITWDYLSKQVNIRSYQEINDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y1Y7</accession>
    <name>A0A8S1Y1Y7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1480113</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000148">
        <property type="protein sequence ID" value="CAD8207773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y1Y7"/>
    <dbReference type="OrthoDB" id="299034at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000535">
        <property type="entry name" value="MSP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00635">
        <property type="entry name" value="Motile_Sperm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50202">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="MSP" evidence="3">
        <location>
            <begin position="4"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="150"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8207773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="207" mass="23838" checksum="B2F0B5A0AC67671A" modified="2022-10-12" version="1">MEQLLLIQPEENLKFELENNQYVARIVLQNVSTIFVAFKVRLSNQNDYSVKPNIGVLRAGNNVTLLLTTGNTISINKDRIQILAGPISNNEDVIEYFKKQQRLNQRIIKCCTDEFSSLQSTVNKSTIFPQEINDFKVTTIKKEYVKPQKKQQQSKDILNLEEQIKKISEDLKVIKEKQNDGKITVGIKQFLISILISMIVGVYITYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y7H2</accession>
    <name>A0A8S1Y7H2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1540136</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000154">
        <property type="protein sequence ID" value="CAD8209461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y7H2"/>
    <dbReference type="OrthoDB" id="322861at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8209461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="164" mass="19873" checksum="0956334FF79616F2" modified="2022-10-12" version="1">MIEMKDKLYLKIINFNFNLAEASYADLEKLVQILKGNTLNNWNALKNSYLLKSEKVMNWWRQEIKDFNEKLKKEIVSLYKFQQNQQEENFYECKEDLYEILFTQKIQMDKFSKGLWIYLKKEKISDCLKLLSNMDSQKLYYQNIDNNIKMNDLQNNILKIAKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8E6</accession>
    <name>A0A8S1Y8E6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phospholipase A-2-activating protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1550089</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000155">
        <property type="protein sequence ID" value="CAD8209643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y8E6"/>
    <dbReference type="OrthoDB" id="538223at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010992">
        <property type="term" value="P:ubiquitin recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015155">
        <property type="entry name" value="PFU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013535">
        <property type="entry name" value="PUL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849:SF0">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09070">
        <property type="entry name" value="PFU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08324">
        <property type="entry name" value="PUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51394">
        <property type="entry name" value="PFU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51396">
        <property type="entry name" value="PUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="93"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="212"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="PFU" evidence="5">
        <location>
            <begin position="349"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="PUL" evidence="6">
        <location>
            <begin position="477"/>
            <end position="746"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8209643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="748" mass="85160" checksum="7B0B653804AA84DC" modified="2022-10-12" version="1">MEALQYKLSQTIAAHNGIVRSISTQGNELLTCSSDKTAKLYEMKDNQYQQVTLISFFEKYIYATCARVNGGYAVGHDNQIYLLDNEGNPLGILEGHDQQVCSLKSISENLLISGSWDATAIIWDISQMKQLYRLNGHKYGVAVYGDENLNFITGSQDGILHSWSKETKIKSVQAHKDIIREILLSPLGGYITCSNDETIKLWSKDLELIQTFLGHKSFVFTMKIHMDQVISGGDDRLVIIWNLDGTPQQTIQLPDTVWTVAINNFNDIIIGTSDGKVRVFTTDKNRYATQAEIEGLEQEASLSNAKQEGGMSEEEVQKLPGIDKLNTMIGKKEGEIRLFRNGNKPEAYMWSLATKNWQLIGDVIGGKGANSKKFFPGDKYFEAGEYDHVFDVEDDNGITKLLPYNEGESFYDSAEKFCLREGYSKHYLQQIVNFLKKNTSFGQSQRQKKSELETMKEQYAQQQILQQQQAKKQIDFQYIPYTQCTYYENMNLQGLSKKLFEFNAQMSEELKLTEKETLIFNKGIESLGQVQVQKTVNIENSVSIIFIQKLLKWDAQYLLPVYDFFRLFCLHHSSEQLFAGLEKGMNLFLNIFTIVNTQPINAVLVRLALQTLCNCLKHNTNSCAILYHLRIVKDIILSLLDTDEKTVLLLSNLILNLSIGIYQRNGLNDQASELLSEAIITFLQYKQRDVETIAKLVTAIGNLMKSPAKQIREQCKKITFEFIQSLVIDTNNQDTLKCLEEVKLSMLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y9B4</accession>
    <name>A0A8S1Y9B4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1620027</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000162">
        <property type="protein sequence ID" value="CAD8211076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y9B4"/>
    <dbReference type="OrthoDB" id="286294at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8211076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="541" mass="62558" checksum="D4793AD0D1157424" modified="2022-10-12" version="1">MKILIVNGYGKCIKGFRSLEHYQQIIKEVLTGKKEMIDTELEFFFADRDSIDDFLYEIDSSFVRVECGKMFDSIDLIFFEGDANLRPWSPNAYKYLILLRMCMRSNKILFASSFAMQGLVFLIASNVECQFSVINGLNGGQLGDLSKMKKPLNEIRMSDFFLDNVTGDLYSFNYDTEEWISKGNAGLHSRRAAEEFKTIGKYIVKAPQYKVSRMKEIDQLYFSKETEIVCSLRKNSMHNYLFNGLPFEFVVPFKNSWDVHPFNFVNPKKTFQTLADCEKGPLIIQVSDNIIATQFSIRSKYKETVQIMRNFMGYQLTKLCSGRAQTIPIEIASIKQNDNAMDIYLEHLGRSKYHNQKTIKFNVITEFHHAGFAAKKSNQLDVVVNNAIGKRKFKQTIQKLNPKELDRLLEQNSSISYQPFLVDKNHQPSIYSIQPQHNVEEQTLIFNKTGAEIIQFLHPSIDKNIFPNAKPLWVPGYLSQSRLQKSQLKQTQNTTGEEVQSTPFLTEQKQIITPKIPRTASQPNFRVIQKDKWITNKNFKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YC22</accession>
    <name>A0A8S1YC22_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">KATNIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1630018</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000163">
        <property type="protein sequence ID" value="CAD8211309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YC22"/>
    <dbReference type="OrthoDB" id="304622at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026704">
        <property type="entry name" value="KATNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027859">
        <property type="entry name" value="KATNIP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21534">
        <property type="entry name" value="KATANIN-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21534:SF0">
        <property type="entry name" value="KATANIN-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14652">
        <property type="entry name" value="DUF4457"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KATNIP" evidence="2">
        <location>
            <begin position="146"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="KATNIP" evidence="2">
        <location>
            <begin position="379"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="KATNIP" evidence="2">
        <location>
            <begin position="469"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="KATNIP" evidence="2">
        <location>
            <begin position="735"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="319"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8211309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1067" mass="123714" checksum="E35540FD69884980" modified="2022-10-12" version="1">MSVQRLPLEKQATPRHHSSKPIQQVSSTARTITKPPIVQQEMNPDNTILSFMKSLDGKQAAILNRSIRDGEKLHKPLIKPRTYIAQQKTIPTSNVERQILNNLETRIQKLPQYEQELVLQILASIENGQPKHEELISFFKSKRIHIKIHSNYGNKTQVGLTSIELLNNNKKYVKINCIYSDDDSHNTINNLINGHNLTIYPEQMWITNFKQFPITITVCYLLQEESEFLTSVKIWNFNKNRKELDKCVCELEILQNDQILWSGQIARGVANTFSEYAQIIELEPQPQQIINTIKDNETRIKNQNDTTQPFNQSLQSINSKKSDENKEINLSKASSQQKHAKPKLIFDPFDDDKKFKKIEQKQEPSSVKTKQTMHQQRFFKKSINLFSQKQLTEEDSTSPQPNFKRGVAHIAAGTISTRTKNISIPECPFGSKLIIKFLKNWGDLYSIGLTGIEIFDYKGKKVKINSVSGFFRNAAVLFDDNYLTQDDKNMCIERIDKNMEITIKFNDTKLSMIRIWNYNKGRTYRAKCVRNIEIVLDTDSIFSGEIKQANGTCGIDNAEYIMFTNNPQIMEKIQEQDWLNKLHESQIQQQKHILENTVKLSRPDTATFNKPKGEITAQKFANNNLNSQFNKDYQHQKSDSIPQIIRQESESKLQQHPKRLFSSQNQINVKTLPTVNVKVLTLYLIRNWGDKFIGLDKIDIHDKFGQSMKIKKYKVITSQSETVVNSDERWQLNNGKIKIQFVFIQPTQISRVAIWNYNKEDELEKGVQMINIKGDNQILNSKQGIYLRKGHGLSDVNEPQLIELPYQRRIEIVKVNYQFERNIYQDYETTQLPQGTKIVIKLLSTWGDLHYIGLNGIEIFDPNGEQLYPKLFAKPFSVKELPQMEMDVRTPDKLFNNINITLDAKQMWLSPFVNSYTDRLKTNINSSVFDQISYQVNKLILLFDTPQQISAISFYNYSKTPVRGVKECEISIDDNIIYQGYLNLSTLTQNVRGIMGNNKTTVLFTRDERLIDQIGTVEIRESIIGSETLLINENQKLKYNSIKNKEVRLQQQGLYKELDRPTTTQVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YFR3</accession>
    <name>A0A8S1YFR3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1600064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000160">
        <property type="protein sequence ID" value="CAD8210692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YFR3"/>
    <dbReference type="OrthoDB" id="293033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035874038" evidence="1">
        <location>
            <begin position="17"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8210692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="307" mass="35579" checksum="FF66A00D3E3F7D99" modified="2022-10-12" version="1">MRRLVLGLLCVVLMTARNVILQTADHGVFTEVFKTYAKSSEFGGRLFAELLMMAQKGATLQNLNDHVEELRNYLSDDQAEDEKYLMINNKNRNLELRLSVESINARIKVLNQQQQIIEGLFENDQFAYQKRLGDQNVILELIDQLIQRLNAMSQDGHSLIEKDNLLDEIKALGRGKHVEVLAQITSHLGNEYIGRIMELLSKLRDAVLNSLEADEVKEQENIRLYNKLSGEIRNSFAESTRQYQELDAHYQSQEYELNVKMEGVKDVGQKLQDRNDDRQFILDRVQEAQKLLNDNLDRIVRWSIKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YJY1</accession>
    <name>A0A8S1YJY1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1650089</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000165">
        <property type="protein sequence ID" value="CAD8211882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YJY1"/>
    <dbReference type="OrthoDB" id="290592at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39867">
        <property type="entry name" value="HELICASE ATP-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39867:SF1">
        <property type="entry name" value="HELICASE ATP-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="142"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8211882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="938" mass="112113" checksum="9F178EC8CA843F61" modified="2022-10-12" version="1">MSNILQRMVTKEVKRRQVDSRLLNKSQQTLRQLIPDLKSPIKSPQVSTALEQFKSKFTEGKYIFQIEQQRKSQLDEVFQRVQIPIKRDFLDVLLQQFETELWRVCNEQNLQYLSDRYLSYQLSNNKFLKHLVQFVDEQREKAELALGQLAGKEATINELQNKYAQLQMMFQQLSHNKDDGQTKLNKNESVKRQKELYMENVRLKNEIERIQDKLKQIENQTNVQKLQEQYQELARKSSEKINELLNENQIKEKQLQKLNLHYQNIKSQYNKLENESKAYQTVFEANKLHLDRLIKENEIHTNLMNRYREIACMQREDFEVRIMYYKEELSVASKAKDKLMQLQSKLDRFQIEKSKDIEPLPNNNYEKEKTGLEKFNDLFSNDKTYQRLTYQALQEEKGRIVTRVGHSIVTMEQHFNAQEIQLEKFTIGYCPFINLVEHHKKTLEQEFSGLQYKRPNLQLLVTLRVILDSKWNEMQIKPEKMWTHLPEFAYSHLMNFKVDMQTKSIKKIDQDVHRIDDQIIKFIVDFSNPVFEKNWECITFQEFLNDFTSQDEIYFYLYARNLLFRGPECSHTTAFYEPHHYIPLLQAEFVATHLLSQYETATLQQVKKVLREKAVNKTITKNLFIVDASFVLRILLEFYRVERRNRYKMFKIAFGSRATNISFKQFRVVLISNYPYITDLELATLYREAYSFTGTGVSIDSFYTVASETGFFVKHLKLQNLTTQPKIVNKEFVFEPDTQPFLIVNEAYKRFQAYQNKFEQILIDFGLEELFAQFKELDNLIQQKFAPLQHNQKSLSQIIESYMSVFRNINNLICNRLYLQDQIQQGQEFLIKHEEKMKLNKEQNKDAQFYSGDNFNKQYLRREDFIPDIEILKGQMDCLEHLGSYLESYELVKKRRDYCMTIQAKKIQKFVKKKMNKFYSFVSSLLSAKFKASLKQGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YKR8</accession>
    <name>A0A8S1YKR8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T2140007</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000214">
        <property type="protein sequence ID" value="CAD8214440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YKR8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8214440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="36" mass="4332" checksum="AB91311F59DB1B3A" modified="2022-10-12" version="1">MRRINQKLLRFKKIKKKRNCRSKQNGIRISGCLQTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YKX2</accession>
    <name>A0A8S1YKX2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T2030013</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000203">
        <property type="protein sequence ID" value="CAD8214283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YKX2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8214283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="570" mass="67842" checksum="4C7547D9063F5E1A" modified="2022-10-12" version="1">MELLINIKQTQQFDLIISGSEDRTIKFWIKKNEWLCQLTIDDCSNRVLGLSLNQQKNRVVSCGNDQPILIIENQVRLQSGKQYKRFKLNNNNMFKFSQRVKEQISVFGMNPNNEQFTKTRDITIKCVQDNFSRFSYQYINSKCILVSKNGEYVNLVRKQQNGGFQTEQSIHFGINSLSILILNKELKCLEHNKQAKVHAYKINAEKICSRADRHLKTLNKQFEKRNTLFQQYLFQLLQARKISQQLDQRGLNQINSNLKIIVFNSNKFYGEQITRFRIDQQFIQFEENLKKIQYSQKDSLQEVIQTFSSEIYYSSRLLNQNWRQENHKLQVALIDLNEQAVVPNRLACIDFVAKYPIKDTKFQQLKQFQLDYVDKTLKYEEQQQINLHEKASKAIHANLVQQLFSTMLLRNALRYRAKQKLINLKTKNWYQLEQLEIIEISNLISKTKKQDILNGSIRNEFKDYKLNQKNFLDSFKTFQENLIAAFQQIYEEIQIYLNTVETDQYPNNSPDTESENLQQQQNQKAMKQTFKYEIINSVKDYRIENRCIRFQLRIILFLQLVILIQIKLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YMA6</accession>
    <name>A0A8S1YMA6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1670030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000167">
        <property type="protein sequence ID" value="CAD8212214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YMA6"/>
    <dbReference type="OrthoDB" id="293403at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8212214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="89" mass="10688" checksum="C80CAA519D6F9C31" modified="2022-10-12" version="1">MQQFHQNSLSPLIIIGDVDNLEEDLKSKHTPEKSPSYLDFNNQKQENTEKKENNLMIKKFSQHASDFTDLLYLWNDYLRNKFEKNNRLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RZM2</accession>
    <name>A0A8S1RZM2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0020092</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8132830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RZM2"/>
    <dbReference type="OrthoDB" id="291186at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8132830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="218" mass="26049" checksum="AC0EE86294EBBFFA" modified="2022-10-12" version="1">MNKGNSKKRTSSFNKQPIIYTHDRCDTQQKQFYCPKSKKDVKNLISDTKLKSSYENTMVIMPQNVSFKNVLTELAHKSAKKYIKKHMKKTPSTSLHTPADFTIRKTEQQSYLMKQQQSIIHQQSQQTSYRSLLQHNKRNQWIDLINQKSSNYIQSDQISTTEYKTQLDIMTTNNLDWKYPYTCYKEQRIHRDVSHIENLEFDCWKELLMNRLILRQAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S0J4</accession>
    <name>A0A8S1S0J4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0030494</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="309148at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="124"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="327"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="663"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1271"/>
            <end position="1895"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2063"/>
            <end position="2131"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2194"/>
            <end position="2228"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2278"/>
            <end position="2363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="13"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8134771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2410" mass="287609" checksum="55D54F67E90743DD" modified="2022-10-12" version="1">MNKKPLLPRQTKPTSSSPPSNSGNTLLDRIKSLNQRQSDQVKQLTHTSSFTTDRVKNRTRANYSEYFDSRKLPFNSTLQPSRSSSCKKKRHFYDEKVESDGKISQLEDQNHQINDFIANIPLCKLSKDEQIEKLELEKQNLHKLTAGYQKKAEERKTIMKQLYSILKTKDQEIEYLRKNYENQILIQQQKLEQFKHECHQQLDKADGRIIKLEGNLQCALQYRQKRLNDLLVKEEKMRDILSKLIKKQESKEKIDEVKNILAFIAREKSRFETPKKQNSQHEQIDNIQNEHQKLVSLHENVMLQIEKLNYQVSQCLQTSQYGQKDTIKELSQNLKEMINQFSLSKNNLKDKDQKIIQLQNDLMKFKKLSENKKDNDTQKYIQVINELKLEQSNLEKKYQQLIIQEKFGEENNQELQNLNDQLNVQIIKQQSQIQLLHKQIDDQNKVMMTNEKIIQQYQQKEKQLYEELDNQKEQQEEINQLKISLISLNNQKDHLLENIEQLQNEIISKNDVINQAENQINQQIRQLNKGQQDLIKYENYNEELQQRNKVLQEKIKKIEDNNLQQILQNKLQDINNKLELLKNENESLVQQIQILQDEKDKFFDQLIQLIKQKQNNLLYKSQSPKNAISRQSSKFGDQLFISDGNNKQISKFQQELDKFDEIIQSINSKLIQVEQQKNEFRNLNLEQQKQNSQFLLQISNLEQENKKYSQMLQQWHQQMTGNISVNLDQNQDIIKLVKQELKKLKKLEDEYSQLKQIHSNLNKVQEELNLEIEEKNQLLEQNKQQLEIEQQQLQNAIKENLELKQQNQTQKLQEVNKQLEIQIENLKNKEKEYQIKLENLKTQNENQDKKIIQNQNLIQQLNDKENSHNQQNQQLHSKVNDYFQQIQEKSQEVEKFKMDLLSSENRVKQQELIVNELKNEIMLQINENNNQKAQQQQILELQENLQTQKSLNQDQLVLIQKLKDELNELNQQLAQKDQLLDSQSNDNIQQNQLLQQQIISQQDLLKQLEQQILENEIVKKEQLDKFNETSTDLEQFKLNLKQTEIEKDQLSQQYEDSQIQINELLQKIGLGEQHNQKLKDQIEQQQQKLQDNLTELQFKQEEINEMAMKEQASKFELDCLKAKCLSYELQIEDQEKLIAQQNNQTQLQSEIQEQLLDIQKNNEELQQNLNDKQQDYDILQNSYYDIRDQKQKLEEEIEDKDQEISILNRDLKIYKEQIDQNKALINQLQDQIVQFRTSANVNFAENCKLNEQVQMMKKELENYQIDSKQNLDERSDKIKDLEQQIIDLQNELENQQFQIQYKDSKIEELNNRLQETLENLDDVKQELEITSRQLKSEQVQKEEELKCKQQLESVIQQYKLEVQQYIQTINQQQENLLSLHNKIIEYCNEIENLKNQNSTMQIQFEKQQKEIEVLNENLNQQNILFEDQKQSSENSEFQINYYQTQYNEIKIEYDQLNEKFQDLEQENEILNRNQSNNLQKLEQIQNEKYILQQEIAQLKQSLATLFEERQNNQDRIQELIFKIELQKSEDAKKEQKIEMIANQQNDLQQHLIELQEQNKNYQQNIEQIQIQLQDEKLITENLKLQNEKDQELIDKLKNIEIVQLNQQIDNLQEIVKLFEQKEMQNQELSNQMSNLRQEQQIQEQQVQQIKEENEKLKEEINDLKKNQINLLESEKITYEQKYHQFQEEVKANEISYQQLKLQEKQLIQLNEAKDEQISNLTVLKADFEQKLDQLNNEIKLNQEEKQIQQNKLQQLNEQQLKLENQRKSQSIELEQLKEQNKDLQQQIENNTCQFNEKIDQLTNQINVLQQKNQQQQNEIKELILQNEEFQDQQLSFMEQLQTEKQAHNTQLSENHQLQEKLQNELENAQQLKGQVQKLEQELYQLQQAMIDKDESLVRWREQCCSQYMQNEQIGQSQNTQKDNNNESQKMNNRLMQESLSNPLEDQDNDYRVEMKEHENSKIFDLEFDDKSANQQQNILPAVSENVSEAYDLAIKKSMGLSKDQIIQQLQDQNRIFQQQIENLTKENIIYESPCKDKVIIEDLQQQIQQLKNQQLQRIILSPLEVLIGEHLDEKDELNEEEKNYLLQEKCKGLELEIQMIQFDNKETQKINESLKKENDSLQLQVNILNKTVKVWQETTTNIHQQYDQKQLITFSVLQKALNQEQQWGQQINQIGLSSECESIFRQVCQTVYGVPSLQKQLEDIKKQLDKEKQEINSLINEKVVLSQQIIDITSQHENSSRRNKMSEPNSALKINQSIDDDEFSLNIKQIILKYKKEQEIMKIENHQLTLQLKQHEQNEEIYMKQNKLMKDENRTLSEKLNQMQDELQKMAEKVSNSENGALAEHLKKNIQKFFEALQQEKKQEFQSYSSHLLKLSGVQFEQQICESLINKLSSVIDKKKSSGVFGGIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S1H4</accession>
    <name>A0A8S1S1H4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0030132</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S1H4"/>
    <dbReference type="OrthoDB" id="303581at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="33"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="144"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8134098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="200" mass="24223" checksum="06995C67FEB483CB" modified="2022-10-12" version="1">MYDEADGEYIEETPADDGLEDVEVIVNKEKQYQQELLQVLKQKEDEHKELRQELKNLICKIQNIQLKQYQEESPMVDPYLREYMQELSEQQLFDLESQRQELKGEKQGKIEQLRQLQQAKNQIKLFFTQTEISSHLQISYKNMLENLNQELFQYMQERADLEDEQFEFDKRLKSLRSQIREVTEKNQKIIQQIENSKQIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S320</accession>
    <name>A0A8S1S320_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0030407</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S320"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8134638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="193" mass="22839" checksum="058391788DDF6FAE" modified="2022-10-12" version="1">MDVSNAELLILKQKKGTRLASFSEENYSIIWTFGENYVLYPERKLIGFKSKLNSIMDIVYSFHLLCQSDTYLQVINYHDNIIAYSFDFKYQLIDFLYVRKYEKFATICQGDIISYAVQKLIIIYKELCYQLIVNFIDVLMDQSNFLMFLKISQLMNINTRNANSQIEQTFLLVAFAESRKQIKAIKIQEDQYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S494</accession>
    <name>A0A8S1S494_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0030267</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S494"/>
    <dbReference type="OrthoDB" id="293248at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8134362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="172" mass="20633" checksum="5716F0BA2E457C6B" modified="2022-10-12" version="1">MNITKQKLESLFDLSIIIEKIKILDEQQSNMFHLTKRPNYQLQKQSNTEFIPHIINDFEIKRKFKEIQNDSFLSWKPNIIRTNQKISQKRPSQIQKVSPSPKPLDLSVEKKIHSTTFMIKQNNKIRTKSTDQRIKLRKIETKDIEAQFLETRNLINRFNRIKRKAVRFNILT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S920</accession>
    <name>A0A8S1S920_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0060259</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S920"/>
    <dbReference type="OrthoDB" id="300043at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035795266" evidence="1">
        <location>
            <begin position="19"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8137741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="390" mass="42975" checksum="30DC22BAB96ADC37" modified="2022-10-12" version="1">MQILLLLTILCLLSTINASCELVNGQCPSECNQVKGECVAQNCFDFTAKLGDSQTNPFKGSIAYIDGTIDLNITITYTDPKFSDATIKGITKGENTESCLTLKLYKYQNSDHQIRDESSQTINYINDGSSTRGYQFTIPSKDFVKELQLSDNGTSFIFQGYYGFDFNIGSSLQRVLYFDFLAGIDRTSTSNLDTSFGSLSQKVELNCTGECVTSTTSSLKFCSDEACASPTDKADLYLNDQIWLKHTLTKDGTQVYYLTNPEIHFTGDKLFKKANIISSKLNQKGYAMYLIKVEIAWRSVTISAKATISSTAGSRILDESSRLLQENQGSTQGLTSEIECVKDKDSNECIDCDESLDVNNIDEPQEDGCPTGSSGLSLFFGSLILVTLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S9N2</accession>
    <name>A0A8S1S9N2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0050346</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S9N2"/>
    <dbReference type="OrthoDB" id="291199at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8136788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="128" mass="14962" checksum="C510291D0FE68122" modified="2022-10-12" version="1">MQQDKEISKNWLRVDPFDTPVLKDAPQHQPERFSQYIELKDENDNVIGFQVDEQQYIFDLEGGWRDENGCHYDKDGQPAGWFVLHPGDIHHEHFYDQEGIYVPSDNENSDNEKVLGGDAKQIQKDEQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SDV5</accession>
    <name>A0A8S1SDV5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">S1 motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0050379</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="412781at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:003532">
        <property type="entry name" value="Pre-rRNA processing protein RRP5, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045209">
        <property type="entry name" value="Rrp5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23270">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 11 PRE-RRNA PROCESSING PROTEIN RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23270:SF10">
        <property type="entry name" value="PROTEIN RRP5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1 motif" evidence="2">
        <location>
            <begin position="108"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="2">
        <location>
            <begin position="445"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="2">
        <location>
            <begin position="536"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1128"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8136854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1457" mass="169181" checksum="5BB3EB98195764BD" modified="2022-10-12" version="1">MKKKVIDALKDDKYMFNPAPTYKNEKGTEDKLKKKKQREKKQLQRRAQLAEYISSDDDGPQKQQPILDQDDYGALGDLDQDILGYFQMKSQKILNMTQKIPDQIFDIGSLHLGCVSQIGPNGAYLIINFTRNKKGFVDQHNLRDRKSVNGGDYKVGEYVMTKVIEEHKNNKVQLSLHPNVINDQLSVNQIVVGMQISGIAQTWNEFGTTINFGTEQFSGFINEQKLKCGRVYLFNIKEINLKEKIVICDFEQREVQLQNKKQISKYLLTPGNIWKCNIAKSITGGQIVKLNKFGVLGYIFQDYQVEQEKNFQCRIIGFDEPSRQIYLSSKQEHIDNTIYIPPYEVGQQYQGVVINQQLYSGAYLFNAILNDDELSTKKSKKAKKGQQPNLGPICMLNKTQIPADQEIIDHIQRICIIKEINYFDHIGFVSFEVNSQPKISDLKVGAIVKGVVKQVLLKEDSYSVLLKINDNFNAILPSIQMSDYPLTNPPKFRVGSKIRVRILQIDEQHNNIIVTMKPTLLTDIKVFKTLDDINVGDTLYGFTIKKLENGILVKFFQNIVGFLSNFSLDGQNPDDIKDGQIIKVYVKYLNQSENKLLLSLKKIDPKQKQVNEGQTQNVPLIKQVKTKLNLGEKVQCIVSAIKNNVVYVNLKDGKFGRIHKAFFECYRNEDRLFKLQSEFENLNVGSQITAQILGENKENNKQIFDLTCLSDHIELEKDVQKDSNKIVYGIIKHIKSDSKSPLVVQIYYNYNVFIDGWDINVEQPDILNNLESQIQQGHPVKVKLYEFDKQLKGIIITEDDQVKQQQQNINDILYTKQIYRILSHHDSYIRVQLKNRQFASIDITELQELWELNLFEQFPVGRWGVCRIIAKDKVYWASARETFLNDKLWNKCLHPVKSNTISYQKMILSLKYDVRTQLYKKTPLAVGQVAMGYVTGSTYNGTFVKLNYETTCLIRGFKTDIKYRPIVLMITQLNKLQGTVDLNLLKYKLEDKQLRIGDIVQGLVVNIRNEKAAIKILGTLQQGELDKKDAEGLEGEDGWWANKLFKIGQQIQCLVIGQRQNKNNQTVLRLSNLQELIKTEGKRQIELGDIQQDVIELHESFEEMVKQHQIKNNVIVEQQNQNKEIEEEIGQEVNEGEDNEDEQEDVEEEEDHKDQEIEEENDQEEIREEVQNLESRAEYEKKILTNPNSSVIWIEFVAYAAENEGIESARNIIERALRVINFSNELERLNLWTAYLNLEFNFGSEDNLISVFKRGCQNCDGKKLHIKLINIYRKAEKVDLTVELSRSFVQKYKQSCKSWMEFLQSLMEWQKVHDDENPQYSFKDTLNRAMQCLKKTKQVKFLAFYGRLQFQNNQIEEGKTTYETILDKNSTRTDIWSQYLDLVIKYCQPDVVRSIFQKAIHNNKKPRKIKFLFKKQLEYENQYGDSATIQKVKEQAEQWVSQFMQRDAQQEDEEDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SF55</accession>
    <name>A0A8S1SF55_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0080291</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SF55"/>
    <dbReference type="OrthoDB" id="312082at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000616">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="11"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="35"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8139781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="503" mass="58350" checksum="01EFB172373AF79B" modified="2022-10-12" version="1">MLSLSIFNDRYTAQKKLSSGSFGVVLLGLDKEENIDVAIKIEKEENQEVKSLEREVQVLQRLSNTEGVPNLYWYGQQDDYNIIIIQLLGKDLAYYMKKKRRFSVKTTIQLGIQIVKVLERIHEKGVVHRDLKPENILFGQDNESSKIYIIDFGISKIFQDKNGRNIPFRNGNSFIGTTRYASIAAHKGYELSKKDDLESLIYVLLYFIKGQLPWQNMQNVTDEERTTKVGDMKMTIDPRELCKDVPIEFPIILEYIKALQYSSKPDYSYIYKLFQKSADGLGIILDYAYDWDIPSQRKICSNDMIKSQEMHRSNTLNQALLPPNQQEMKKSLEKGNKNLMRQSSNNNFLTPPQNNKLQLPLLRRSDSQQPSVASNQGSAMLSTYNSRRPKYEQSLVVLDQFEEVKKSIDSIQLEIGNQNKQQIEEYKFNRILNQSLQNPQISNIQQSNYYSETVGWKDSIFEENYLDDPLSKKYGLLKNNGVDIGKKVQFKNNNKFKQSKKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SFA0</accession>
    <name>A0A8S1SFA0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0070154</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SFA0"/>
    <dbReference type="OrthoDB" id="293577at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8138516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="333" mass="39291" checksum="559BAB9A5C0100CD" modified="2022-10-12" version="1">MIIKNNEQRTSINKSQTIIVNPIRKSQSIFLTTSNQNIMSFKEISSKQQQLNRVLNYNINYSQSVNTSMYSIECSTTKSKIRNRVNQGDFCSKFMQLDKEIIEVYSEFEKNYRSINQQDKLEHIQYQTNKYLEVNPKSIKQLIQTHDGLLQSDLMQGDQNHIKINRFKFNYFRKKLKGKVSPIHVFFNVPDRVQTSSLKTFISIKAEFPTKFNAGYIMHSRFAKIFSEKNQHYFSEEYLFITRYSDVDFEFSINFSFGNSQIIKSPIKQQHEPSELIQDIQPLIQKPQARDKILGNLSVTDYQQDTFSIIRKIIKKKAKSMRQSIIDHLKEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SFT5</accession>
    <name>A0A8S1SFT5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0070293</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SFT5"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="129"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8138748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="508" mass="59305" checksum="D356F3E7BA6C7C35" modified="2022-10-12" version="1">MIFQIGQSDGRKFWCTNNIPNFDIDEVQNVKIANFYKIGKTFGQKCLRQYFLIKQYLFYSDVGNFKNIKGYVRLDGVYLQFQTVNQEYQIYLRNNGFQIILCTDIVEQFQEWSQMLSLKCILTTFEKDYKIGALIGNGSFSTVYKCWNHEGELFAVKAITKQSSKQLKINKQYELQLLSEINALREFNHDNILKLHQVYENTEKLYLVTEYINGYELISKATSKIPYSSNELQSFVYQMLMAIHLIHSQNIMHRDIKPQNIMLRNGQLNSPILIDFGLAVSTQVKDIPFPSCGSLGYSAPEIIRFEETKKQYNGQCDIFSFGVTLFSILYGYNPFKGHDQKSTVKRNADAYFEIPSSQYPPELGNLILLMTKKYPKDRITAQQALNHPFFQVKFYLQIYLPMSIITKQQYEMSKNDKNINVHTSLEMDWQFGLNNQFLNSSCSPQNQKNRTSTVNSNPQRKLTIPSLDQIDEFQLDVFEDSIETSHIEKLKMNQNHFYNQKNLLNSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SKV7</accession>
    <name>A0A8S1SKV7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Aurora kinase</fullName>
            <ecNumber evidence="11">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T0070421</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="11"/>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8139004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SKV7"/>
    <dbReference type="OrthoDB" id="302269at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14007">
        <property type="entry name" value="STKc_Aurora"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="33"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="363"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="425"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="162"/>
            <end position="163"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="9">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8139004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="525" mass="61399" checksum="FAE44E7619165C7E" modified="2022-10-12" version="1">MSQPVLNKVRERLFFLSPTVQAGWEPESKREDFEELKILGQGAFGVVKKVTHKKTNIVYAIKIVEKAMLKKTNMVEQMQNEVKIMYSLNHPYILKLYNHFEDDINVYLILEFVGGGQLYAVLWRQPQKKFDEKTSAKFILQACLALENIHSKNIVHRDIKPENLLLDEKQDIKLADFGWSNFLKPNEIRQTFCGTLDYLAPEMLEKSRQHDHQIDIWAIGVLCFELLTGLSPFAPQININNQTFVEKTTKDNIINLRFQFPTSFPPLAQDLIKMILVKEPGKRSTCQQIKEHQWIVQNTQHVKIPASLTVQQEKKIIQNPEIESLQKDGKLVFTNDQIYSFSRPDSIIVKDFLQGDNTNNKLVEALNKKMKEKDSKIQALEIENQKKQQEINEHLSTIDLMKKSGQASQEIQSMQHKLKLQDERYAFMKQQYEQQLQKNIELEKKIRELESQSNQIEQMKSKMNKYKEEAEIFKTRAKKAEEQVQQMVISGINEKEFQEVPKQQEQVSNVDDLLKKLQNKYDTKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SPG7</accession>
    <name>A0A8S1SPG7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0090031</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1 10">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Endoplasmic reticulum membrane</location>
            <topology evidence="2 10">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the OST1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SPG7"/>
    <dbReference type="OrthoDB" id="310030at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007676">
        <property type="entry name" value="Ribophorin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21049:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21049">
        <property type="entry name" value="RIBOPHORIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04597">
        <property type="entry name" value="Ribophorin_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="420"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8140354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="449" mass="52011" checksum="9E68B4810A0E23A5" modified="2022-10-12" version="1">MLLLIFLALVSSLRIPKNFVISNIERTVSLTDRYINIQSKITFQNDGVIPIEKIYYTIHNALSDRLYHVNVEESNAKKLIEDVDVKYKQNATVYEFTLIRPIEPSSKLEVELSEHFYKRFKPLPKEIGLEDEQLVQFMDSVYFFTPYLVKNQITYYETPKIISYSLKSATQRNQLLEFGPFQDIKPFSTQIHTIHLENNTPMTIFTKASKIIEVSHWGNILIEENYSIENQGAKLKGEFGRVNFNKYNPNVGKHSLKQLSASLPYDSWGVYYRDEIGNISTSNAAKGKLNGETQALVQLRPRFAIFGGWNSNFTLGYNLPTKKYLEQDGNNFRLTLNFSFSVKEMVSDKYNFQISLPEGAYDIQVDLPLYVERNDTLLFSYFDTVGRPTIILDKATTSQFDNKQIIITYKFSGVSILREPLMIFGTFLFAFLSIIYLRRLDLQTLKLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SQC9</accession>
    <name>A0A8S1SQC9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0100489</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8142150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SQC9"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8142150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="188" mass="22850" checksum="4B6BC16337E733D6" modified="2022-10-12" version="1">MIQFFITDSIEVLYYHCLYIFAWYPEAQQEIREEILSACTEEQILVDKVKQLKNNLYLLMKLKDQEIQLYLDTLSQRSQSIKNQNLIIKHFLQSVSEQHFINLEDFYLKRWLTPKQILDNIFQDKIESYFTLPSFLDRSKELQRMTLMKTKFIIQPNEFVVPKWTLRFLYQLEPSNCVRLIKIQDNHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SXH4</accession>
    <name>A0A8S1SXH4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0150306</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SXH4"/>
    <dbReference type="OrthoDB" id="8119704at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46118">
        <property type="entry name" value="PROTEIN ABHD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46118:SF4">
        <property type="entry name" value="PROTEIN ABHD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8146361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="370" mass="43302" checksum="E01F04BD7B9CC821" modified="2022-10-12" version="1">MYRFRQLWKFSTLQFHKGVGEHQNVSQNVVLRGVTLKGRYNDLPNMLFFPEACDPVENWIPFFSDPSNKILDYRNVHILSPRNFGTSDRHYSFDVEDLANDVVRYMYYNKITMATLAGHGFGAKVALAAGCYHPERTTGVFCIDYSPMDQRFHEAFTEFRGFIKKLSGINTKEMTKSQIESFLKENIECVKWRSIFSDNLIKLPSGQWDWKFALKFLNDNISFNKADSLAFWPVKAGLYTGRAHFAFPEFSRWVHLGTNTLPMLKVCPQVRGFGHDVHSVQGDNNTLNHWIYEFKDQSFVFASRFTKFLSMYDGVHLLLKDRTEVGKEFVPSIIYSKKDPNHVYSDYSPAHYYHNWRFNNVYKNLDNQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SXI7</accession>
    <name>A0A8S1SXI7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0120242</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SXI7"/>
    <dbReference type="OrthoDB" id="301897at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="484"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="576"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="635"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="293"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="378"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="500"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="576"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="635"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8143464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1129" mass="131781" checksum="860AB677328C3FE5" modified="2022-10-12" version="1">MDSTLSKINNMAEKAMLELQKQPLITWNPSCLNTFKYILDKLKSCVEEVSFNLMSKDLNSEASLLQKLSTNYSIVFDKVLEINSRKIVNAPELPIEYCQRQPNSTRTVLGGTEVQNFKIYSQKQSDFIEAEQTFKMNKQKVFRKMENKSTSTSDDAMIKLFQGNIQNETEKELLRIQEENEANLIDLRKQLKLKGMWSEITSSHATQKLNDLNRHMERETFKNDLYITDQIKQANEIKDDEEFDIKKEVKDKILLVQRKTAERIMRAAERKIRLEYDSFVMKKTAGILVKKSKTQNFQRIEELERLIKDKNEQIVEKEAKINQLFQKFEEQEKKFASLLKQIDNYKVTQKIEVCNTIVSKNGKKSDVEVQVSMMDPRVELFELRLQEMMEENDEMKDELKLLHEKALEDKMQSLNGPKQIEEVLLLLLQSDLSPDNKIISLNTLLQYIKDNSAEDIARLAPNELYRIVRSKLKFNCESPLEKLDELSGDEDDSYEQQSITKKRGRNKKKRISHIEKPFDAQIQEQIDLDKIATQRRLAQSETDDESSKIQKKQIIQLNQIDDITKKTNVNKLIEQVSSPKSTNRSNITFRQKQGYLKTNRNNSAINNDSSKKDQNSSSGQFGIQNSRQTLTQLETKSNKQSFTNFSAKELTNQKGEKQHKKPSIMDQSDIQCDFYKQNKTKDIGIQVSFKQETQIPIQQQQQKPIQLPIQQPQQQEQKEKIEEQTKKLNESQIYRAKAESISQKITQTDDFFLWNLFQKMAGDLNFTEDQLKKLETVFLNEQQFIHFYEKTQSQKQISRNSTLQVDTLGDYKRDSLKNLDKSIGIRNSIKLRTSSQQNIYTNNEILDNFAYQKNEIGEDDQQKNQNTQPTSWHSPVVSRQQSQIVFSGIQNLNNNFGQINQPWSPNGIGNGLRSNSPMNPNSSQGMRSVSQLINTGDNVHIITYGTVSPSDQINKNKHRTTSSSQKLLDDMLKSKTQILKSPEQQQREQKEQQLFFSVFGDELQNDEEFDLEVIRQNMGKPLEVIQDHMKEESIKKIFTQLAQKEKNEWKDKVFLIISNYCNKSVQTITYFDFKKYYENYMRVHKRCGDECIHIKRFLARIGFGFISKRKILNMSKQSVSPFEQLPKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SYX5</accession>
    <name>A0A8S1SYX5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0140229</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SYX5"/>
    <dbReference type="OrthoDB" id="306474at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035746730" evidence="2">
        <location>
            <begin position="21"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="53"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="256"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8145410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="357" mass="40804" checksum="D5ADBF32B3BD3F90" modified="2022-10-12" version="1">MKTLFLIALIAIAFTARVQERSLSKITTDLKKSKYGSALLHLVELHSMAGGPVQELIDAIEELINDLEEELEELEFNFQQRTNEHNSMVIGYQQDIQDAVIDVNNTQDTLDNLLFPRREQLQVRIEQIQENQEANRKNYDEAVLTREQEHEDFEFQIAELNDATAAVDDALALLSSLTNPSLLQIKRFQNSLKNIEKKIKSRSKMAPMIKALITLASNQNFSDQGIIGQIVDALNEFRNAIVDSINAQTAAEADAQTEHEEYLEQLDAEYAEFQRQINRVNVDLTATNEKIDQLSEFRDQREADRKQYTAELELENNTYAQETDIYTNLKNEFTRELGISEQALSVVQSADFSNIQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SZ96</accession>
    <name>A0A8S1SZ96_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0150273</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SZ96"/>
    <dbReference type="OrthoDB" id="287711at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="285"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8146295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="662" mass="77641" checksum="AD971B17CD88266A" modified="2022-10-12" version="1">MCAYVITFPIWKGQQWSAFRGHIRLSNEQVLIARFNDTASIEQELRFLEQHQRVKSKIEGKFIKPVYNLLPMIVQVIKTPTFTDVLFDNCVDWQLTGGNLQHLLRIYFAYDIYKGQPPRDCTLENIMRNGDSIVVLDFGLRKRVENYCAYWNPMILKGKQCLSQYQWSLGIMYLVMTNGSFIINEINKRISIWLNGGKLDLVSLVTVKKQSIISLIQQLLNPENPIPWEQIPHHSAFRDDPKCKAILKLYEVRSMSDLKIRPVSRLSNKQSSRKTQRNSLVLHESTSNSNQSTSNQSLISKISSLYKNKFIEEYKQRCNSSVKNNKQINNFFSNNKMKLKFATKQSDNTVQLSCDFRSLRTGSNLSNFGLTQRIDDSDDKKLNRRLSQQEIEYDKIRSRVSSIRMAIKNYKARSLRHLSQDSNVPVQNSEEISHPDNYIQLIKRPQVEIEILTQNYLRSLECINIIGQTVAKSIEFLDALQNFWIIPLFLVFKRMLQLRLEVEKFLEAQINSFNIGQWKEVCNSREYLQFLTRIKSDNYLVRNEMILLMNASHTKADQLDKTKREKVLWFLNDNLDSNIQPICLSYMKDQLLLAIIEKRGLQREPIEWLKLQLSLKASIIITQLPVLVCENNEFSYERYLQYQDSQNYAQIQKYCEQLKIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T0R9</accession>
    <name>A0A8S1T0R9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0130150</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="301971at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="170"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="236"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="279"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="352"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="384"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="414"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="446"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="476"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="507"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="557"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="591"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="642"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="665"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="704"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="737"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1281"/>
            <end position="1300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1372"/>
            <end position="1392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1413"/>
            <end position="1434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1440"/>
            <end position="1460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1472"/>
            <end position="1492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1498"/>
            <end position="1520"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="916"/>
            <end position="950"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8144282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1543" mass="181158" checksum="B950618154A1D645" modified="2022-10-12" version="1">MINQEESNNQISDPNEQVSKYQIVFSTEQETNKQECIIKLKDQNIKFFIPETDEAQELCALLDNKKQLQDSQYPKYVELFLQKYNEFEDRFNAFFTCSPRQFKISFDLNQLIITIVYWSTFFSVLSESIAIENNQIIKNDTIRQQIAHQLPVYAFLYTISSQICQQIKNYLIWIFLFIGLIIIPAFVLLGYHLNIFGNYDGFSQDYIGIGYCILSFVIFINSLINRGFLEYIRKVSSAVIATLFNCSFCVLVSYFILFKIIAFVLLIQSTQVEYIIDNISTIIAPSTFIVIFLFSIFLLKGLFNLPRKTIKYDLYQLSLQKYADDDIKNEFSNYNMNKRSRQLFKKLKLQKFVPIMITICYAFIEIFCYANLIIGLKSYDFEQIITFGFLILAIPIFIIFGICISNSRFKKQNLYLYIPLIFGAIFSVICIKIWSLTIENQYIKSISRLIGICPLIISLMWLTVGFFKSEKRNQKLFVMLFSCFSFALPFGILLTFSVYFNDKGLEIGAFVLASAGLIPIILLIIYYIIVVLIYLVKLPKQAENLQFVVYEYINLNSYAVWYNSMCFIISFYLICYFAWNEPITATGTKKGTIIGLLSIHTILFILTSHALSLEVKEQTQQKIDQQENLTYTQKFINRMQDFTTVGILLPFIVLLPISQSVESEVTKNALLANTIGIPLSFLYYKFQISLKQDSFIYQQFIQPLVVIVGWTFIIGPIGTIFPILANYFENSSTEFALFAQLAVAYSILIIIISITVLSIFYSVLLNKEQLEMKKKEVLRAVMKEFSENGVYATEDISSLLFVRYVQHRNCIKLEKDLIIGDPVNMYEHPEPAENNNIKFILVTKQDYENKKKLEYIQERKRLKIRKSLSDNETDMVSLTSADTINQKNQEEQHGCLYQVQDCLFSCLLCKFGYYDLEDMEQNEINKQKDLQELKKQKQCFEQKIELKRRIRQRHSSILQQDNKYLDQYQPIKVDSRRAERFWIAVYHYFFNKGDLSNILEILKLEKDYEILKQYQSFEENNGGFILSEEDFALLIYDNYRDENYSYLEIIQKLALQSYYHLVTDHPDFYQQITKFKDVSSVIQEQNFIKIERIKEERKCLNKILVPEQLQTNKLNEILSSEYKDLDQKYQQKILEIENNYKNKIPHQWEKKIWEICKQIHIAIWITPKKYTQKLMNQLADLYSKIAPIEPKANPINEDWSRIAQKICRVLVDEFNKFDKKSNEQEFHFKLTITNLLAAFLRVYDLYGLITLSFDSQVGWFGESSEFKPIKMVDYSAIWSEYNFFFFLALVMSIAYIVLGLQASKQIADNSFGFNEDGSIAGIKSLRFWLSKTIQIISGQFIFVMKTYIDAFICDYSEYPYTLVRQPSVECMSDIHFLYISLAIVGCGIYYPLSTYLQPTFQFMDRSLDLKYKSNFVVLYIQAKLLILGSSSVFSNLQEQAYQYQMLFSFAVMSALIYFHFRLDPSYIKWFNIVDRSLLLILCYFYFGAFIILSSKSVLAGWIISLIFTFGTLLYLIYYLIQQIIKYRRIQQNQIRPIQSQEQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T4P5</accession>
    <name>A0A8S1T4P5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0150406</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T4P5"/>
    <dbReference type="OrthoDB" id="307314at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8146559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="115" mass="13642" checksum="4FFBF84FCF170359" modified="2022-10-12" version="1">MKQLNQYQTESLTKMGRENIKNKLKCLIPNYNNQKLIVNKEKLINQSSTNTHDNSKISVLIKETNQMNLKIVSKKWLMKKRLLQQQLPILDRPQFVIKPQIILKLFVNCQTKQKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T597</accession>
    <name>A0A8S1T597_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0160147</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8146978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T597"/>
    <dbReference type="OrthoDB" id="291144at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035739355" evidence="1">
        <location>
            <begin position="16"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8146978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="131" mass="14402" checksum="ED74EB09087DB7E1" modified="2022-10-12" version="1">MKSILFITLLFITFAADPEQCLKEKCPNEYAACQKEVFGCASAAMKCKNQCGGDDAECMLNCDLASKNAKLIALAECGHANCQDVAFTYCDIQICVQSFQSECMTSQGLKAYQCAATFLQRHSECQCITEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TAC1</accession>
    <name>A0A8S1TAC1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WD-40 repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0180005</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TAC1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002141">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25173">
        <property type="entry name" value="Beta-prop_WDR3_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="414"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="462"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="504"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="546"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="588"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="630"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="677"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="719"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="761"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="803"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="845"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="887"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="929"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="971"/>
            <end position="1012"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8148319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1070" mass="120202" checksum="029C0FB9535C34AC" modified="2022-10-12" version="1">MSFYQPKVIEKEKNLLCEQHLQQIEYVNLDSQLSTNQRLLCKSCCQQNPSNAKIVDYQQLKQDFDNQQKDRISQYQQIVQIHIDNIEKVQNIIDSVKTKFIQSLDDQLMLLKYWINQLINSISQYSFLEELDFLTQKHRPITNPKNIIESIQKINSDCKFKLDQKQEQFKFLPEIQKSQEIFEDLKNYCRKLELEINSQWMKLSTQQEEQVYKCKEDLYEIIAQSQDLDSSIMTILIKIFNNEKISDCIGFLSRNDNHELYQQYFNEEMNLQCVLRNIKQIYNLMNNINQHDFSQKDYSKQLYQEKYLINKIQQDKKIIEFLKFFVHLTAFDSKFIQCGSNALSLLVELKADLDNQCFENIRIQNTILIGGNFVRCNLSGSEFNNVDISGVNLNGAQLHNCKWKNIKIHELNKLDGHVGPVSSVCISPDGNTLASGGGSIYGDGDKSIKIWDVKTGQLKDTLKGHTNFIMSVCFSPVGNTLASCSWDNSIRLWDVKTGQQKAKLDGHTHYVMSVCFSPDGNTLASCSGDKSIRIWDVKTGQQNAKLDGHSEPVMTVCYSPDGNTLASGSWDNTIRLWDVKTRQSKAKFEGHNDTVRSVCISPDGNTLASGSDDSSIRLWDVKTGQQKAKLDGHSGFVVSVSFSIDGNTLASSSGDSFGKDSSICLWDVSTGQQKAKFMGHDLKVNQVCFFPDGNILASASEDKSIRLWDIKTGYEKAKLHRHDSQVNQICFSPDGYTLASASEDKCIRLWDVKTGQQKAKLDGHSNGINSVCFSLDGTTLASGSDDKSIRLWDVKTGQQKTKLDGHSGQVQSVCFSPDGNIFASGSDDKSIRLWDVKTGSQKAKLDGHSNGINSVCFSLDGTTLASGSDDKSIRIWNVKTGQQKAKLDGHSEPVMTVCYSPDGNTLASGSWDKSIHLWDVKTGQQKAKLDGHTSTVYSVFFSPDGNTLASCSGDKSIRIWNVKTGQQKAKLDGHSEPVMTVCYSPDGNKLVSGSLDNTIRLWHAKKFQQNSSNQESAFNYITTLIISQKAIFQAQEAKILKGEFINQSGIDLKTLFKQKGSCFLESLQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TER8</accession>
    <name>A0A8S1TER8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0200230</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TER8"/>
    <dbReference type="OrthoDB" id="305682at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="343"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="70"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8150378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="515" mass="61936" checksum="28E9D4A48F762164" modified="2022-10-12" version="1">MLQNSCFDDNVGMEIENQLGTRDESKNYDENQRLNIFQTLKKQDYLEPEIQLNESQQSPQFILQYKNQRESTQYQQNSENENSAKQNEDEFQVNINFTPKKNECLFTELNNLIVQAQERIQNLSQLSPNTNDISQCNGTYSEIKQQIFEIEKVYKSKDDELSLLNLQLDKEFNSCQNQKYLDLPTNNQINQSFEIYKRYHQIQEIKKNKLQKFKKRYHEKYNLLKSDLNQLKSIVIQLFQKNQFTLENEQCFLEFAKMCYTKSKQIEYIDNSNQELQSQQQESLKQRNSQYLDSFILTLMKQLNINQGDIEKIKLQIIQCIINQKDLIQQYQIQLETIQTNQNLSTNQQINKLKKINDELENELIQLKQHKEQQQKVLDQNKKEIQELNYTLEQIEKMDLNQQNSKLLQYEQISMKHQQKLISITSIIFQQLIESVSYKSQIKKNLQENFQHLNTYFNKANENINKQIWKQDEDPIKYLYYHLQTVQKMMDIICAEYSKMIREIQQQRQELISQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TJ81</accession>
    <name>A0A8S1TJ81_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0210136</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TJ81"/>
    <dbReference type="OrthoDB" id="425490at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8151029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="296" mass="35104" checksum="EC5036A47D99358B" modified="2022-10-12" version="1">MSEKKIRKKYHDKIDFKLDLPDTNNKKEKARQSAKNTRQRKKAYIESLEQKCEVAQLTAQLNQNKKVESKPSLMQLMEQFLIDRKQQFDMLEKAYQEGNEASIKVITNSLRFKYGISGQGRLMAINKVFELLIDLLLPMHMRYLFWVASEDVDIFKRERQSGEFYSFDMSDSQINCLNSFGLDDDQILKIQNMQQIIQEQRSHYDNLIIEIYKIQQSIIKESEKCQTIVDEIWDVLEPKQAAGLLITLDKASLFYKNRVVLNNLERRKYILIVQYQHFKRGQKKKFKDNNNVLILT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TLV5</accession>
    <name>A0A8S1TLV5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CHHC U11-48K-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0230226</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TLV5"/>
    <dbReference type="OrthoDB" id="304195at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022776">
        <property type="entry name" value="TRM13/UPF0224_CHHC_Znf_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51800">
        <property type="entry name" value="ZF_CHHC_U11_48K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHHC U11-48K-type" evidence="4">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8152758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="149" mass="18013" checksum="E8D054AB48ACCDB2" modified="2022-10-12" version="1">MQQQQQVCCPFNHLHKMAPNRLIFHISRECQERKMNKHMYRHCPYNFLHIFRGEDMEQHIMICPDKKEPDAPIQLDQNNMENQYLQGDPNWGLKNPNNMAIEMAINNNQVQQDSSLIDQFNNLKLQRQDDIWDQLWKQQNNNEDDFDMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TP61</accession>
    <name>A0A8S1TP61_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0250172</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8154070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TP61"/>
    <dbReference type="OrthoDB" id="10494753at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8154070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="99" mass="11671" checksum="601E080DB634F588" modified="2022-10-12" version="1">MEMNTWQNQNDIADSETIPLQSEPLMGAICQRLHQICTTSIQIRRRIIQKRRRNAIQNSRDQNKIENSKETKQCLSQLMLIEQLCNDLQYVKELVSLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TQC1</accession>
    <name>A0A8S1TQC1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">FYVE-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0240050</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="303837at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00065">
        <property type="entry name" value="FYVE_like_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000344">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057352">
        <property type="entry name" value="TPR_TmcB/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25474">
        <property type="entry name" value="TPR_TmcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="609"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="669"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="702"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="752"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="796"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="819"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="851"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="881"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1565"/>
            <end position="1586"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1763"/>
            <end position="1785"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1866"/>
            <end position="1886"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="2090"/>
            <end position="2109"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="8">
        <location>
            <begin position="14"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1503"/>
            <end position="1525"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="332"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1504"/>
            <end position="1516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8153139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2150" mass="253395" checksum="D948914BE8D9EA63" modified="2022-10-12" version="1">MKQFITFINIPEVRGKIPKCQICQKSFSFTTREHQCKRCLRAVCEGCSPFKINHIKKDGKESTKPHRMCKICKEESEQIKNFVEQNHIAYGVDTLSKQWLGGTLSQQDYKNARESAKIRFDKTEISGFDNINYSLKEFYYLVGKDNQKLQNVCLTFCSKNPEVQFSAELVCLANFLLCFSSEASTYQLLTIIYKQKPLDECIVSILTYCVKGYGLGEDEKQLLKQFLQSRLKRYLITYSINLFNFDTTLFLITQLIKKYDSFIKGLSAIFILASTQLKNTNHEDLELWILRNVRRKDAENKLNSMQMPTPKQEIESRDTSQSVRSIAFSMIEQQDQSQNNELKAEISNLKLQLALKDNEIESYKFQLQQMQSPDDSKKDKYIQQKNDEIAILLARVDALTLENKQLSKKESSYLNQNSLQAEQQKIIQEQQQQIERLKTKLKDTTFENRAMSMSLATSAQSIMSGKEVQDLKQQHENEKQQLFDQIHTLEQNKAQLQRIIDSQKQLNQRVVDYISSMTINTNVIELSIKPPLERSTKNDNEDYKFLIDTKNQQNQILKQLLMANQQQIQELNKGINQSNCQFSEIQILQVILCLYRVVYLILKTWSDYRWLTILLCLIQYLQYTYLNFGLKVQYVWQNKAYSDLINLLIKYFTITISVKNIGIQNYLSVFYICFGLIVLSILLIMLLAFVIKNQKLQKPPLLLLKFILKIILTIGFWPIVRMQFGLLACEYNKQGIYVMMFEQSQECWTSEYYIHAVVAIIGLIVTLLFSVLMAFITYESRNTNQDASAMRNGRSYSILLVYIFVQIMVYQLLPTPEYTIIIIFVFLIGSSYIFISTHLEQPYYDNVIQKIWSISSAVNLWTIIMMFYSYKFDGQTFQKTVQAWLIGIPLIIGILAFRQQKTVDLFVANLKKFKNGMQIILLCEYLVFLMKRSEKCQRSQLLLDSYIELHKEICDRPDCSVKQKIELANKFKENPIYTDRNHIIIELIIQIYHEGIKKFPDDTPLRLSYIYFLFNQQRSYSIIMIELNQAMDFKPTLDYEFHIFRYKTIIEEESEKNAELFDTANQLNQTKEFIEYLEIVTLKVVEFWSALSEEVPDMSKLMVYGFKIIKLQEEIDYKWNKLQDNKSLKLYNYLSRFYQLVLDDQEQADNYQQLYWTLQKTKQQELSELEEICNQSTPTLVASAQFQKVNIYAVNKSFCSLLGFSKADLIDRSINQIIPNIFIEKHDQCLELFIENKARYSKDFITQSVYLLTKSNYILPIFSTVKLLQAQNNQLFFIAQYQQSFSPKQLCYLLLDIKGYIEHISSSCISYLRLDIQRIKMRKINIKELFPDFHQRKDEFLSKSGAKLYIKSHGMKYSTKTVDTTLSCYDQIEFQCYLSYIQFPCLKDSNKYGMAIKLEQLKINNVESPCMNLLPIQQKRTKFPVFQYIPPTVYYMDYLSLTQIEESINNSHVEENASKISKIADYFLMAKKMTQSIKHVLNYEDGIKTKRLWDNQIIDIQDNNNEDSEEIEEDDNEQMKKSQKQEKEIEIQEFQHYFKLFQSKSSIKKLLINSHISKTLKLTNLFIQISLFILYTSGLIVFTFNLNDDTKVSSMIFNLSLTNKRLSSCLMINAVLQDLKLIRFGEISNMRMDLDEFFTYNLKLLDQEIDELISIHSQLSIIDTYINEEYQIYETIYFTQNVKLFSSDGTSQNYTYKEAIDQLIAKAIQLNQNQISLFDDSNVDYFYYNYNILNSIIQSYQIPLNYLYYSIKSVAENSLDNKIAFLILQTFIQFITYLLIAIYLIHFSRQLQQIYLFYFEINEQNIQKVIQNCEKFLSILNVGDDEVDEEYDENLQHHNHEKEEIKMLNKKTKKVWKNSNAKFKKLMITAFFIFLNLQVYFVYQYYSAQQVVEDTYYLCPILNTTSLLESQYRLGDNAIREYILNPNQNIFSTSPQTFIQSYLVKLQDVNAEMQTKFTQNVDLFQQDFINVFQQLYIQNPCPLLGQLQNFINEDYCNTILDGTVSEGLSIGLTKFYENLRILLIDYELQRQQNQTEIQNQYYGILLLSTNFSLEIRVMQKIIIRYTQRYLIEKLEKSISDSFNNLSITRGTLFICFTFYIFIISFFLWIPSNTSLIKSMQKSRTLLLLMPMQILTNSTPIRRYLRAVLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TUI8</accession>
    <name>A0A8S1TUI8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0280258</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8156315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TUI8"/>
    <dbReference type="OrthoDB" id="311990at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF9">
        <property type="entry name" value="IMPORTIN-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8156315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1023" mass="119164" checksum="96E1DF0AFBD319A3" modified="2022-10-12" version="1">MASEIYDQLVTVFTNTDNKIRNETEKQLVTNLIDNIQNFQQIAKVAMQQDKMQEQAASLLNSVILKMLTNSIQLSIHHANSILAVLMSQYTTLKCKQSLIKCMSLIVRKDKSVKTEIENKMKEYLRQDQHWQFQIGILFFKILLDSLELQTYSSIVQTGYEWISEFFNLTAYIITKLTEQPKELADDFITTIRLYTQVVSDLCEKVFDKNNSQKEQTQPIQNILFQLNSFSGALIILLSYSPQIGKQIQNCSIINTGNDHFDNQLNEIKCQVFKIYFLTLQVLLNTRNKNEVKKGAFGPYLTTITQLLIYSLLSYTNTESIHQIYNKPYLPTMMTYIMKLLANLGTQTEQYQIFSDSKIALITDAIYPFLITSQKEYQQMKDQPEEFVNLALDSVDKQQSDVPKTAAASLLEALCDHIDGSTTFLANLAIIISQHAINMISNNPIQLKEQQVTFVQALQDKKLFKEYSPVDRIESSLVILTIMSYLIQKRLDIVLLMEQLLSDNLLFFQNVQDQIIKLRLSLFFGYYCDNLFKKDTQSQNMNAYLQIMISFVQPQEPVVLYQSIDALKDIFEDDDLKIKTKDLVVLLFPQLCNALQYSTYERHFETITNLLKRYPYQFLQNDNLLVGLIQVLTQRVIIEQQKINSGDQQRHIYLNRCWNVLRSLADQDEFSEVLGKLEQHLGQLYSMLANCDKIDFDEDLVLFISGCISKLSIVTELQMQILPYFQNIIKKQQGRLCNLFETLNQYMHFGRNYFINEAQQSIYFQLAFQNLQNEDNYGDIEQGEGALLIQLGIQELHDDLKSNILSQILFQTITILQKPDINEHLKSRITGIILSSLYKIPEKTYEVLSEVFPAVYNRILDTQYSPGYDIKLFIITMSSLIMKQPNLLTSNLLTIMVNNLIAQEKYEKDQKFKQNDYMDDELDDEDDSDFNDEEEITQAQQQTEQFLSSIVKLDEYSLFKQTILCFKNLYPQIIDQLRNGLDQQIINKINEQLQFIRVETQDGEELRKFRQLKNCNRKKQFNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TXP1</accession>
    <name>A0A8S1TXP1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cache domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0270288</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TXP1"/>
    <dbReference type="OrthoDB" id="298276at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="413"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8155779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="560" mass="66091" checksum="335C3084BDFF6FBF" modified="2022-10-12" version="1">MIKHRRLFTFCLNNLKLRTQLIILVSLIIIVIVSYVLSVNLIHYSLFKQYFSLISNQIFEDNSFKISEKSIRSYQGYFDRVFYLNGNSLVSFHRLYHMTKKQVKQHSLELNTDYQMFYGGINKIPDPIRIIKGYGNSDISYSCMCFSNITEFQNPLSLDELIGMKIQEQTQAYASILYQGNILSQSFFYSYIIKEKTNAVYPCLNRGKGIYNYDPEKREWFVEVRHNFNKKQPYTEYSQSFTQPYLLFTDKKIGLSLVLPIVDENLELIGGVGTNFLGAQIVQIIKSQEFGFQVIYLVSDSGIMIMHPYEVGVEQLPLYIYNQSITGFNTSDWERMNSNKQSQNDSLTCPYLHKHSLSLNCLYNSLYQQEMIIGIQEIPGYSMKLIMLLSSQEYFQFYQHFQEDLDKKLLNQLWLHISILLGLFLFICCLLYIFTYTLFYPIQQIQALTINRIFSKNKKKLQMPFYANKFMSKQITLLCKSFQFVQDKLEQLSFNKTKSCRDLENYQYPQKKFSLKKYISKEDNFNKQSNLYKEQKLNANETLTQEMIIMMLRNSQKFSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U0D6</accession>
    <name>A0A8S1U0D6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0300129</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000030">
        <property type="protein sequence ID" value="CAD8157393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U0D6"/>
    <dbReference type="OrthoDB" id="296958at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF37">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT PRKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="382"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="9">
        <location>
            <begin position="641"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="174"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="743"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="36"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="180"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="743"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="763"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="411"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8157393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="892" mass="104202" checksum="2B23CBBCA2B9649B" modified="2022-10-12" version="1">MKKISSQIQSLMNRNSFQEPSLSNDIKSSRSNPKTAKTERPQSKTKKNDLLDYFKKERNTITQMVHSVKNTLQGSKSIDILHTQSQKSERQKNGQLNFVDLNVQNEDGNNTKRQILKQKNLSLAIISLENQLFKQSQTTKQSYNQSPQNQIKSPIIKQKDQYLFDMQILKNTKSSQDEYQQNSQIKQNKQSILSDDPSYHRQSIEDMLYNKKHFNHVQTQKQYPHKQCENEKSKQINQIHFKTEICSSQIQSKYQNADETLNYDQDQQLKIILYYKQQKYHYLFKYQTKTTDDLFNYLIQQIASIEKQFTKLGGGTGSTDGDSIQQELNKICQFQTVSKNVPYDYYLTLNELTLDVFKGITLQIQPFYSQTIQTKRVGLKDFTLVKCIGVGGFSRVYMVRKKDNGKFYALKLIDKKFIFENSKEIIVQNERDIMSRMNNQFVTPLHYSFETKYYIAFVLEYCAGGELFYHLRKLKRLNEQDAKIYFAEICLGMAYLHSKNIVYRDIKPENILLDLQGHLLLSDFGLSKPEMTPDDFAYSFCGSPEYMAPEMLMKTGHNYLVDCYCLGALLYELVTGLPPFYSHNTQDIYNSILTEQIKFPNYIEISPLLKDLIHSLLQKIPEDRLGHYNGIKEILNHKWFQDVDFDAVLNKKVKPPYKPYPLKYNFDEEEFNKGDAEFRKQFQINLQKEFQNVDDANYLLDNFYYSRDAVYGQEKSRRTNVVNLNQLQNQALIAEPFTINQQTKIQSPQSESNNEQLFQQTSDESKPINETKGKNDIQDNSKKQEIMTKSSQLQHQTAKLGHTYSKTMQQAQSSLQELKQIKLLIDQSRAQQTSDRITTMPDQNNKKITERFKTEQFGNLASPKTTTASSSLHKMSNFQALFGSEKHKKKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U3U1</accession>
    <name>A0A8S1U3U1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0330149</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="289913at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045111">
        <property type="entry name" value="Vps41/Vps8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616:SF8">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 8 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="1244"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="1331"/>
            <end position="1365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8159450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1386" mass="163083" checksum="2221F54C0CB3A0E6" modified="2022-10-12" version="1">MEIFTFDNCDHSKEFQLLVQYYSVFDEDLESSNRTKSIVDLLDAGLEIQEKFRKKAKQEKQTQKNFKTPLDFLRDSLKNKIDNTLSFQQILIFSNPQVIKNFGKPKVVHHNGEFLIVGGSLGNILYYSQSQMDIIIQQQKIGVVTALDSLNELVAVGYESGWISIINMKKKKHQYTCQNITKSKIIVIKFIFQSKNFFNVITSDDMGIMRIVNFRKGILGFDYQIQLTIPRQQYPMLNIMVLYKNETKEFMGDQYEKSSRIIGLCSLRKFYLIITYKDDEKKLKSIIEIENPHFSGPEKPFYCYLSYGYGILPPPNQEQEQQWKEKKLIILLCWNCHLFILVKGNDQARYMTLKPLIFKQQIHAAYFVEQSIIEVITTNQNIYLFNTLHLEMHMVPKGIEQIPYFIQKKNWPDSPFTSLYQVDIKNKDDGIELKQYPLQNQIYALNKQTRQIHILVEGSIVTGSINNLEQSIDIFIQNKDWAKGMLLILALMDGSMKSTPIKFNQQVLKNKVLEIATHYIKVVLEEIKTTLDKHKTLIYTPTSLLGHQQDVEKVKTLLSMLVDFLLRVSSDILFNQIYCIIKKFLNGDISILCNELSLYLKNGKVKMIDANEQTINEILLYYIKKGEQQCIDAFIQKIEYSKINTEQLIQQCLINKLTTSLSYVCTRSGDFLTPLAKLWGIIQQDIKKKDPQLVIEQGKNIILYLEYTLHIEELIKEKSDQISLWLFEIQTLFLLMSIDFAGSVQLCYRFFISPYDQFLDKERLKKYYINMTQAFQKLKVIKDQNDLKFQLTQLQIKTLPDAIAWDKMINQVGRSIQASILSKGYTEQLQNKQLTELFLDLVSEHRHLIFLPLQCLPLPNILHNDEPTLKYCLIYAYNQFIINLFKKYPAVDKEDVLNSMASDGQFDWVKMYLNEELKIYSRTLDFRIKIKNQLQNFNIKVKFEIFTWISNVLQKDPPIPQIDSFKDRILYHIGSLVSYSAVHTRSLISKYFQANDISILNKMGELPKLQLEYLEQIMQADRKQYQSNTELLKLYIKLICQIKPEKVIKELQEQEYPLDEMIVVLKQYPVPDALVYLHERSGAITEAISVKLDDFIQKIQQKAFLSKSEFYCYFLEICNICLRNQKLDELDESWDMVTQCILELSQNRYTPQPYPSYLEEYIPLLLKKWAECSHLDAFLDKITTKYMRLSSSKLKMTFMSMYKHFNFSSTVYTKLVDINFIKCIKNMRALVHLQLKGQGYLPGCYKCNNYYEYHQQLESIILLACGHTFHYNCLNLDEKDQYHCPVCLNSPKIAMAHQLQQLKSKKYLLKTENQQQQLYQKNQNPLIINIIKQGQKKQQEIEQSLKFIQNENEEENRRMKQFEKLKLFEQQKLIRLERVCQGVILY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U5Q8</accession>
    <name>A0A8S1U5Q8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0330115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U5Q8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8159403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="580" mass="68765" checksum="AEA8092D5AF427A3" modified="2022-10-12" version="1">MQILENSDAIVSDDDSLELDCRIIVPKFKKEEYDTFQTETSTSVMARVKQIRSNSQEFRFMQSNQVSPQGSLNSTQKLIQCTLLNLDEESNKLKVNQHLLPQYEYRNTLEMIRKDNIPQSSAGSVEHVNNPQIPNIQEQNLIRMLTQQIQPFQKQPIIRQNKRQLTQTFKSCPLEASPIKITRFSQRQIYVTQLVNTNQQPLQEKLSLVQLENKEKFCKLSLSLFRSKTSTIKYTKQNIQLKVKMEREEKIVFKNHKIQQKKFLLFKINNQKMFQQIIKKKKQHFINNNSLEYNDILIQLFIQFFMFIYFILFECNRYFQIMNLRLKNSNYLVGNNDITRLRRARLIQPSQPESKSLATVKMNLSPELKPKKQKTIHQDSVQDENIINQKQNEKNKIYQAKLQELLSLRKSSLTPLLKQQINRQQTIIKHQPDFMKDHDLYSKIRRQSIENLQTQEKSKNFVLKSQQECTQKKRIYTNNLKSSSYINGVPILNRSVSQFTCLTPKKKDNNSFSYLQKECIKKQKFEGLINLFININIENLVIAFNSIKSKAPMLPFIKELERRKEDFQQKRFFIQLVKAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U7E8</accession>
    <name>A0A8S1U7E8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome b-c1 complex subunit Rieske, mitochondrial</fullName>
            <ecNumber evidence="4">7.1.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0320035</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a quinol + 2 Fe(III)-[cytochrome c](out) = a quinone + 2 Fe(II)-[cytochrome c](out) + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:11484"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="7.1.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="4">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="4">The Rieske protein is a high potential 2Fe-2S protein.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.8" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8158576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U7E8"/>
    <dbReference type="OrthoDB" id="297702at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008121">
        <property type="term" value="F:quinol-cytochrome-c reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03470">
        <property type="entry name" value="Rieske_cytochrome_bc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014349">
        <property type="entry name" value="Rieske_Fe-S_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006317">
        <property type="entry name" value="Ubiquinol_cyt_c_Rdtase_Fe-S-su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01416">
        <property type="entry name" value="Rieske_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10134">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT RIESKE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Rieske" evidence="6">
        <location>
            <begin position="151"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8158576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="260" mass="29868" checksum="730F49ED788663FB" modified="2022-10-12" version="1">MISRVLRRSFIGKAHFGLVSEYNSRVNQKLYKGVQVSEAPQFFTTSARPGNFGDHIDFKVQMDNWFDENRVHNEHETEIKRTQIYALNAVYYGGLLSFARLFAVGLIGRMNGWKRYDRDTYLEMDIGDLPPGEVMQIVWNGTPVFIRRLTQQEIKDEDNLPKETILDPSSEVVLTNCGNTKVLVVSALCTHLGCIPIPYLGAYNGWVCICHGSVYDKYARVRQGPALQNLPFINNSIYDDVIVCIEEMKFPREPSQRYWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U7G0</accession>
    <name>A0A8S1U7G0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0330288</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U7G0"/>
    <dbReference type="OrthoDB" id="10252009at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000236">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF519">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE MPK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="5">
        <location>
            <begin position="7"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="6">
        <location>
            <begin position="67"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8159717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="204" mass="23252" checksum="3B0DD5C5E5CC3143" modified="2022-10-12" version="1">MQQHCNLILNERGTLWLGDYESALNLEFLKSKGIRTVITVAAGLNLKYEGIVHHKIEILDIELTNISQYFQTANDWIERGFNIGGVLVHCMAGVSRSAAIVIAYLIEKKKMTYYQAFTFVKSKRPQINPNKGFTNQLMSYATRNSQPPIPKSSRANHFQQTQDQFENNFYAPVHRKSSAGVIQQNPNFYKSITKSMSQSKSKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U7P5</accession>
    <name>A0A8S1U7P5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0330113</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CSN7/EIF3M family. CSN7 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U7P5"/>
    <dbReference type="OrthoDB" id="10265275at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045237">
        <property type="entry name" value="COPS7/eIF3m"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350:SF5">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 7/DENDRITIC CELL PROTEIN GA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0736">Signalosome</keyword>
    <feature type="domain" description="PCI" evidence="3">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8159399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="149" mass="17952" checksum="052E5AEC5EE8189A" modified="2022-10-12" version="1">MSLIDYIEDDQTYQFTQYMNLIKTDEDKNLFDRFAYGVCDPQIKYKNEKQQKKMIILTILQLCQRNKCVTFDKIQQACYINSRIEIEQLLIDLIQRELIIGSIDDYKGCLNIQRCISRDVRKSDIPEMKNQIEIMYQRVKHLKEQLKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U929</accession>
    <name>A0A8S1U929_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0340313</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8160392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U929"/>
    <dbReference type="OrthoDB" id="285787at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8160392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="218" mass="25364" checksum="32531D7F93A17050" modified="2022-10-12" version="1">MKTKMKKIFPSNTQNYNSVPQLEHDNGSLYKMHQSTISKPPLSIVPSEANQSYYQIIEKNKLLYLDQYSQYNEMKFMENMAGLRPKSEKLSLLENFLNMFEDMVSCLIVIQNTLLLIDFEAPDFAQIYGYSPNTMADILNKDIKTLENLHLELEFQSSLRFLETVLEALQFNPEQANKIHQLDVKAWSQKCYNHLNNYIGIQQQSNASKQSLLCPCFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U9N7</accession>
    <name>A0A8S1U9N7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0370040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8161815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U9N7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8161815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="210" mass="25515" checksum="43FBA0ED7C8C23E7" modified="2022-10-12" version="1">MYLFQCLKLDFKNQIYVRKLIFCYSQIEENHIQLFQITLTISPLMEAFRFQTFFDSLNGNRKYVIEASNNFKKDNINAYFFDNLKASRSAQLQHISVFLYVSQQNLFKYIYLNSHFQLANYLQILKNNQTLKYLIFIIMQFQDCSSLTLMILVLHVPFKWHILLLKRIIFLQINYYLSFLFYRGVSFFQSLFLIHKPLSNQFEYMKNFNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U9Q5</accession>
    <name>A0A8S1U9Q5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0350069</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U9Q5"/>
    <dbReference type="OrthoDB" id="1918685at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00024">
        <property type="entry name" value="CD_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051219">
        <property type="entry name" value="Heterochromatin_chromo-domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22812">
        <property type="entry name" value="CHROMOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="3">
        <location>
            <begin position="5"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8160603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="191" mass="23134" checksum="EF57173B0A827BB4" modified="2022-10-12" version="1">MEGLFVVESIVGMRKNLSKIEYQVKWLGYSKEENTWELQENLIQNFSDLIYQYHQNEFYQKFKGFIFKYKEIKGNIKIDKPFQIIREFETHFLIAYYPRKNKFVCSSLINKSEAPLNLLQEFYCKQQDQIGYNEYNNEQIGCILEYYQKQSYVVLLESKGRIFINNTIIIDKQPELLLDYFENMNWITIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UCJ8</accession>
    <name>A0A8S1UCJ8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0370063</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8161857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UCJ8"/>
    <dbReference type="OrthoDB" id="668540at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010608">
        <property type="term" value="P:post-transcriptional regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF12">
        <property type="entry name" value="MATERNAL PROTEIN PUMILIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PUM-HD" evidence="3">
        <location>
            <begin position="189"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="247"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="283"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="321"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="389"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="429"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="474"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8161857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="540" mass="63715" checksum="5E0B3F0FC52A125D" modified="2022-10-12" version="1">MNNTLSDTLASLSNDFQGTENVEGQLSDQQKVKFVNLGMMTPMSNTLTYDFEQITLDTSPKESIVNLINYENIDTIYEQEENKCKLQVDLFIYIQKKYQSFKLSQDQIRKSSKHNTYQPQQTMSQLNIGEQNLDTHINQQSPETSPMNAQTLKFIKELWNSEKNETEYFKDIIDDQKIPIIEYKLPSLNIYSPLMDFNSPIYKHDIEMDLYITSLVDSLWGNQIISRKLQKMLETGSNEQKELIVQKLERVSPEIEKDIFGNYVIQKIFECTNNNLQYRIFIKLKPHFYDLSKNTFGCRVMQKLIEYTYNRNDLQLLVLQQLQSNMRSLIYDLNGNYVIFKMLETFDKQKMKFMIPLIEESFNYMGQQIYGCKIIHKIIQQYSQQQITKIIRSSVQNYNILSQTEYGNYVLQHILQYWITSQEKILLVQLVLQQFFQLSINKYASNTVERALEAINKQDLISIMKWLLCRSPNQYTSNFVVLANHQYANYVIKKFLILSDHNVLKYISDHLNQNQSEQIAIKSTVHGQRINSFLDKQIQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UCQ6</accession>
    <name>A0A8S1UCQ6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0370212</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8162124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UCQ6"/>
    <dbReference type="OrthoDB" id="415459at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051252">
        <property type="term" value="P:regulation of RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000003">
        <property type="entry name" value="Zinc finger CCCH domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041367">
        <property type="entry name" value="Znf-CCCH_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF18">
        <property type="entry name" value="PROTEIN TIS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18044">
        <property type="entry name" value="zf-CCCH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="5">
        <location>
            <begin position="17"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="5">
        <location>
            <begin position="80"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="17"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="80"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8162124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="216" mass="24899" checksum="3E4EAB110D91CB2A" modified="2022-10-12" version="1">MSYPFGNQFSAGMPNAKYKTSLCRHYQATRQCAIGAKCQFAHGQEEMRSINDPIPSALIAVLTVPPPYQEIMKPMMPQQPAFKIPCKYHAQNYCKNGQNCQYSHDVDIQQPMIPNIINPYQPQYPQVDMRQEIKQEDPMTTVFIIILQTLEQIFSKDDIIQKLKIAQDQVKCGNLTVGSECIKLIMTDPERTDDEIEQYTTIYNNACYYYHQLQQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UE76</accession>
    <name>A0A8S1UE76_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0350246</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UE76"/>
    <dbReference type="OrthoDB" id="293074at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000048">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="95"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="9">
        <location>
            <begin position="350"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8160936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="631" mass="73297" checksum="1B962713142C8C83" modified="2022-10-12" version="1">MTRAINLKLQDKLILIEMPNDANLSDLFKRIKEHPLTPNPNQLIGLKTVSGALTLDYILSRPEDRSLFPLNKLKQNEWLMGIYKQQTGQLSLKDFVFEKCIGKGGTSEVYLIRHKANARLYALKMIKKHYITDCRRLEQVLREKKILSNVLNQSPFIVPLYATFATREHLCFLMEYSPGGEMFFHLQNYRFTEEEAKKYFCEVICTLEELHKHKVLYRDLKPENILIDIKGHIQLTDFGLSKLDLMNEDVTHSFCGSPEYMPPEIVNRQGYSYPADFYTLGCLLHELLLGLPPHYSQNTDEIFQKIINDELELPDDLTEEVTQLLIDLLEKDVSKRIKDFKVLKKYQWLSNVNWDTIKNKQLQMPIEIDIYETHIHGEFLKVDVTEFNQKNETGDLKPQDDLFEFFNYINPIYQDQFLIESKQQNIKNRATSDLIMEKKQKLVLNEKQQNVNPNQIKKCNSVKQSLKSASSPRINNQLFSTGHHSTQNSPKKQLQLNLQDIDNQLSMKTPKQPKSLTGTTPTAATLALLRKSFQTLLKKAPSLRNQDNNPTLRTLLSERSLMDRNYIQKIIQLQHSSPTNRELSQRPPQQLQKRISSTSTHLKSQLSQLSLFTLSSQITQRGSISHRPKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UHB0</accession>
    <name>A0A8S1UHB0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0400287</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8164230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UHB0"/>
    <dbReference type="OrthoDB" id="297712at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40515">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 157"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40515:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 157"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="40"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="269"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8164230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="464" mass="55237" checksum="5D3CDA6F105C89EF" modified="2022-10-12" version="1">MEQFNGKPQDNILITQPLTKQKYPALFHDERFGKDFESNYSKLQEIMDNLQGKVEQIVKSQEDDFMKAYKDQMNELQVDLKAMKRKIDEESLKQKADEKKRILEEERDYFRQEALRLDNLNKEQLRTIEELKFKLKITIEEKNYYEGFVIDSKKENKALKFELLQLYKQKMEDQKAINRIQQQQQTQQITKLNSKKQSDNRPQTQEGIRSEANLLIKDDFNSEFLRREQSSKGGFRSQLSTKNNDQASTQYDFFRREISTQQQSRFHPENQMIEERNNQIAELKQLLQKEKFLCQQLKCELQRQNSQRGELEVVLLDCVNQLKKDIASRQTVQKQPFLGNTNKTPKVAEQIAFEDIDYRQFTHQDKKSLLKKFLSSEQFLDQIYQLTFNSQMASTSQLKLNEKWKQDANDATKKFNNFKIYKFRNITSQPIVKQVISKQEQNILSDLQEKVDQGQELVDQIVQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1URR5</accession>
    <name>A0A8S1URR5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nephrocystin-4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0460108</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8166867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="313446at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097730">
        <property type="term" value="C:non-motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058687">
        <property type="entry name" value="Ig_NPHP4_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058686">
        <property type="entry name" value="Ig_NPHP4_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029775">
        <property type="entry name" value="NPHP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058765">
        <property type="entry name" value="NPHP4_C2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31043">
        <property type="entry name" value="NEPHROCYSTIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31043:SF3">
        <property type="entry name" value="NEPHROCYSTIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26015">
        <property type="entry name" value="Ig_NPH4_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26190">
        <property type="entry name" value="Ig_NPHP4_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26186">
        <property type="entry name" value="NPHP4_C2_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NPHP4 C2-like" evidence="3">
        <location>
            <begin position="599"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="domain" description="NPHP4 Ig-like" evidence="4">
        <location>
            <begin position="901"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="domain" description="NPHP4 Ig-like" evidence="2">
        <location>
            <begin position="1157"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="433"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="487"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF26186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF26190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8166867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1342" mass="157182" checksum="4576DC11251DF9DC" modified="2022-10-12" version="1">MSEILDDEQAYLKRWYKFAKIHKTIPPPKKDTHLESLCYRIKIRDVYNIPPKKDVQQSIRILLNLYNSNTKEFFGRTYESPFLELNYSQANQQLEHPEVFCYFHLRQIESIQIAAEIVLFEKKDQVIQNRLIVGWCLGEIGQELKYTLKRGSSRFLISNQTNVETVGQTMWMDIVENKEFTQIRNLVPEGCLCGYGDEVLGVHGYQIIQQLSLMQEQIAYVTSIEILIPTSVDLLISEYLKKYKEIKYKDDGPNDVHFLAKRLVFTFHNGWNYTNTRGLNNFITLEESSFQKSIDNQKIDYKKLTFKGALDVNGIYQEPKLGVFIIQLEYDVEFMVTNLGKQRTTVLCGWLPLIPKSNFEEEFIVGPGRSLSNQVLVDLSDIKYPITISGKINFGSIQTQNLPGPIINQQVFQPMDAPSLVKLPEQGGQQLGMDDDQRKIKQLENTLKQLQQDRQVSATNLAMDFENKLKIDKQKEQEYQKMVETIKKQHEEEIQKILEQYEDLKKMMTDINQTQQQYLTKAQPTSASLLGQQQVLQDQKELEKWRIIESMTSVPRKTFYKTQQQDLMVEAQFGGQALSRGDIAFLSQNGVRELFVQHTDYVENDEIKQKSLQYELADHRQSITVFIQLLSFKPRITMKSKDLEPTVYHYSEESRIPSRLFMIVNFYDFPEYITEPLIYENGFDKSSYTRCLEQSIPFLLIREQFLKTGASYKEPVFKFEIEPSSIGYPKAYKYFCEYLFNKCITIEIWDFDSMMIFGTIRLPLRQLLRQGQDASVVMRKADIIDKEFKKIKGELQVLIKCVGKEDKVTEALDNNSQMKRKVISTKRVEPLHVLEDEEKDLIKQDPNERIKERIKRIRRQEVPNDPNNNSTKTLMEITKRKDLSKNAFVHSVIQSHLSDEKVIHPFFGKVEIIELEFRNPYATGQNFVISIDDQEVKCSGIQEIHLIHQPVEWKYYVDLYNHSKPQEFNMIQANQNNFTMFLTAKEKVVLLFKYQTFREVNQEFTELQKTEELFTIKGKQFLTQYSYPRQVAVFINTIEGQCVGGMRIKIFPHNNTIDHVYRFYERDNRQVSIQFPALYGYTVPSPDKKPILFCNNDQLVVEWANEFEIRISLRMPEVNKQTVFYILAYQDTYFKELIANWQIKLTSLAGIGIEVPLGQVQNTKLTVHVEISRQVKFYSSHPICVWFPQPYDQLVNLIPGKINIANLCVRLFTEQIHKVMVTCVDEQSHELVRGWILNISPTVPPISQVFDIQAPRDQMKTYIVKYANKQNNEVLLIFKSSNKQILKLIETRYLFKAQQIQDVTLQIHRVGEMDKAEVKVFIGDEDRSRDTFDCLLFRITYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UXV3</accession>
    <name>A0A8S1UXV3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0500127</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8169033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UXV3"/>
    <dbReference type="OrthoDB" id="301912at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="338"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8169033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="774" mass="91832" checksum="63DC137A6B1D97D4" modified="2022-10-12" version="1">MNQVNMDKLLYPIMMSIRSLRTYFKQEDDNNRIILDCQNKVEAFVLSIMHFLLDLIVNGSDHTSISFNKNAQQLQRELRNKLDCIQDFVQSKDVANLKKNILKFYDFKEGFSESESEDDGVSLQIKLTGKMKEFNPEIQSLRIELDQKEQTIEALKQKLKYRQSSMEQMAHNHHKEVSVLKAHFVLNPQPEDPQSIFDIKYFDSTQMLDPEIVALMNEKINDIKNQYERYVKQFMDKFQKQRVQNNIEENIKQPSLQDFTSKDLLKIALDKENNPYIFFKNIQDLKSINYILNVLSNQQNQYGIDYDKINKAFKTKEEDLKKIIDTRIMMEDCQAQINAIYLVDLEKKQEEIQELNQMITQQDIEYKQQLKLIDDLIQNNNENSKQMILNQQQKIDHMRSENHNLSTMLLNQKQHSQQLKQQNQFLMNNLKHILNILLKKQSQSIQGLDKYDLIKEKSAPIKDILSKLNNLEGYDLVTLIQEVRFILYQIESVKVVNQILGRGKLNKQTQTINYNQELIEYKEEKKTKDKKEQKIVLKHLEEIKQETIKKNKLKIASTQTDIMNEFNSTQYHENIQDQKTIISQSEKCGTVSLNMLLKQLQCSIEQKPNSIRDLTPEPSIEKHNSVFDKLYIQQKQENPRWHQLKPLIEKLTEEEFNEVINTLGIYSSRQQQQQTQNTTMEIPLYDISRVQQSMIQKSSYKKRRDSSVDQTRNVFIELDEQRENALKLFEQQRIDNYRRRIKRLVPVQPSQCLQVEEKLRPKSQIRSTQQKPGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UZK8</accession>
    <name>A0A8S1UZK8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0530144</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UZK8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004963">
        <property type="entry name" value="PAE/NOTUM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21562">
        <property type="entry name" value="NOTUM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21562:SF83">
        <property type="entry name" value="PECTIN ACETYLESTERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03283">
        <property type="entry name" value="PAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035910193" evidence="1">
        <location>
            <begin position="18"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8170608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="411" mass="48440" checksum="E215AB79F2CB9624" modified="2022-10-12" version="1">MFFFYFSSLIYLLIAQGEEESFDDNDFLRPPPDDEGSLAHPLHRTYLQDSYCMDGTQAAAYVFEGSTDDLIMYFYSGGICIEDSTKFLKYGDYAYIDNCTYRNTTFYGTSNGYPEEFNANQGLMGNNKYQNVHLRKAYKMFLMYCDGNMWYQQMNPQVFKGALLQMKLIPKRIILAGSGVGGWYLVNKYNELRRVIKDLYQEEEVEIRILLDSVIFDISRNKDIIDAYTEATKRAGITMNDIFSFDALRKVDIPTFIVHSQYDWWQLEICDGFECIGKIHLDKCTPKEKKQIEKIRLNILQQLKDLMKSKPDWGLWAISCVFNELVIWTESWNHPKFQIPMMKGGLLSDKFQDWLENRVDNNVHYDVVSWPDNKPCSNIYHNGKIDPQLDEMMRNYEIKESRRKREEKYDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V0F9</accession>
    <name>A0A8S1V0F9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0540035</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8170948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V0F9"/>
    <dbReference type="OrthoDB" id="286529at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8170948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="230" mass="26898" checksum="770C7BC1803C7749" modified="2022-10-12" version="1">MLKIEEYVCNLKESKTLFNDALRIDTHQSSESYDNTQFQNPIYNIVPPEYFQSVTENKYSNIFSSYIQSPKHEELQSTIKQAPKKRTFSFAESQQLNKDPSCNISADFGLNNQIPIKGDLKNYSKFSQESNLKNIETPNKDRFQLQDSMLVMNNSIPQSKLSSPQKKYVKVQKFQNTNYSFDAKQTQTTQITNISLKDNREFEQIFKECQNNFEKNKARKTEEYIACNIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V7H9</accession>
    <name>A0A8S1V7H9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0560010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000056">
        <property type="protein sequence ID" value="CAD8171952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V7H9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="166"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8171952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="221" mass="26583" checksum="F2D74D6A8F18A926" modified="2022-10-12" version="1">MLVGKRRQFEDESIEIKNPTYALRRRITQDKNLETLCQARKRLTLQNLAIIQEKYPQIQQSDILRLLEDFDNNIEMVCEILCQKTDVPQKLEIFDTDFDNYKNNLKEEMLKRLQQSQNNQLAQQVIQLFFDVFEQKLGEKYQQNIQRLDSENILLKQAFLKQHKRLEKIRVEGENKEKQNQNVIKQLDEENKQQQQINNNLTILLIQAQQNNTVNYLQYQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V9E3</accession>
    <name>A0A8S1V9E3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0580264</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000058">
        <property type="protein sequence ID" value="CAD8173195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V9E3"/>
    <dbReference type="OrthoDB" id="10475686at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8173195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="129" mass="14777" checksum="C62A07DFC1C702C2" modified="2022-10-12" version="1">MTECTSTNYQNCLILSENQACLDKFSTFYNPLTGRFRTTCVENGQTNCVYTKLICQSGFECQTLANPQQGCFEKCTTNKSSIFYCRQDKSQYCNENQACYDPTQSIGSFTQTLNEIQIKYSFKFLLKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VN74</accession>
    <name>A0A8S1VN74_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0700163</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000070">
        <property type="protein sequence ID" value="CAD8178884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VN74"/>
    <dbReference type="OrthoDB" id="292381at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8178884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="137" mass="15787" checksum="491B0DD695169BCF" modified="2022-10-12" version="1">MDSNLNVIQDEDDFQNLHNKYRELVTFSPKTKSQINQSRNLKTLKLVPFQLISESNTSNEGNKSTNHTSSLQPIQVVQSLNILQNEQPLSRRTCALIEQSTKSLSPTKSSCELASEYTFKLYEGHKKVRFRINERIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VQA8</accession>
    <name>A0A8S1VQA8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fungal lipase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0700170</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000070">
        <property type="protein sequence ID" value="CAD8178891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="310710at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041682">
        <property type="entry name" value="AAA_14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13173">
        <property type="entry name" value="AAA_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fungal lipase-type" evidence="1">
        <location>
            <begin position="111"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="531"/>
            <end position="651"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8178891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1485" mass="175547" checksum="3F3917C7878B07D1" modified="2022-10-12" version="1">MNIQNFQEFFPNDYIHLLLAAFSAEETGTKGGQCPLKISDDVKFKQETEIYKFMKQEKEVDMNEHLKFWKISEIFDNSKSGYLGLLLENKEKRQLVLSHRSTNFKLSFCSNLFKQSGVQCDIMSVVIGVFSTHQAQAYQATQQAVQKARDLKYSLSFCGHSLGAWLAELSVYYCHQLFNPPFPNVKAVTFDGPGSFEMMQQMDQQTIKGQSQLFYPEQFDIVFYLSAPNMVNCMNSHIGIGYRLQQENFASIQLPKCINKVTGMLQTYVDSLETMIGHQLKCIIPMFDHQTGKPKLEHYSRIIKWPSIKYKNYSQQSKIHLDTILLMIYPIGLKILLVKIMTKYFVNMSNYVTSCSVFVGLIEYFRGNINFDQFNKIHEILNKTNQYQPKVKNQEEFDLQIKGGYYMNNPNEYERNTCNPKLIDHIDEHLINIKINTQRIDNSSQERFLIMHYNQAIIQRLNKYQYFINILRDQIRMIIRDYQEDFEQFLNENPYFDNKKITCFFNRLFLYDENYVQRKELEQIEKAFSQSNVVVITGPSQFGKTTLAYQYVKKLKDEINAIVLPFNAENNSTLYSDLQYYHHHLYQIPNQTFTFEYLQQDLLAKIQNTNQQLIIIFDNVKSLDIIQTLITNLKQLHRIIITTVNNQIFSQTNYQLLNISALTEQECLNYINNSIHKRKLNDEQKKMLIQSSNSIPKLLHIYIAQLNNPDNVLEQTEIILKNLNQSNKYSSLIEQIQDDFCIKILKYISWLYPNKIEMHLIQQLLQTTESNKENTIQKGLIKLSKLGFISILGNQCIMIDTVIQEECRRLQQSQEYLLEILKQFYEWLPDIQTEKHTINAQEIAQYYYIHVKEVITLIEQKHLFKYFLNNNFDLFEYATMITLKLSIVYQFLIHDYQSSQKYLDLFGEQYYQAMKTMNSINLQALTIAIINNTQLYLTMNKKQEALTNSVLFQIIETEIFEISPFLKSNMYNQLAILYQQYQQYDLSNQQLEKSKKIKCLNQSEDDLRIQKILMIQAENFLLQKYYNQAQILYKKALQHLQLIENNNTLREVQLIKAQLYYQFGRISFKLKQDSQETQKYFDVAVKIWQEKLNNIESIEMANFQYNIGLVFKQMKRYDESLNALENADQQKKKIIGEEKNEQFAVISYQKGLIMIEMKKIDQAEQELIKAQNIMQISNNFKLKSKIDLQLALINEQKGLKFEFIKALEELISKKRNHYAYIHPSINNDILSLADAYEKILKDTAKQTVLYQLIYFITILHESQIQCYQQKNPITDQIEQAKFQANYKQINANWLRKNNSQNIIKRFQPNFFELDLQRTLILRSGIITKEEFEIKQILQTSTLNYIRQYTFEGKWERSILSGFTLQGVQNFINRKHLNNQLRGELNKNQNLKITLMLCFEAINIGIVQSNFEYISFANRFITKIIRYQQMQNFLEDVIQLHPEYFIDGKILKSLVNDNTILQENLGNEEKKISSNKIISDIIFNMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VRR8</accession>
    <name>A0A8S1VRR8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0740044</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000074">
        <property type="protein sequence ID" value="CAD8180314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VRR8"/>
    <dbReference type="OrthoDB" id="304544at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="169"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="221"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="408"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8180314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="470" mass="55986" checksum="51690E9145044857" modified="2022-10-12" version="1">MNQKSFFTWFQLLLKRDQPIILDQSNWLGFVSEVLDVICSFSMNKIDLKGDIFQRQQIQSIIELLQKLINILKNFDENSINELLKFIFLISKEIPELEQLNLQIDEQDQYQMFQELNLNDNEIIQLSFSKYELLQIIEHVEDKYKQTKEQLEQILPQFEKAEENLKLSIIAKEEENKILNKQIEELIAEIDRLNIINAEQQIVVNQINEQHQCNPDHSQLLQYLQRQHSVIQEQEQELEELRKIFAKKQLNNIFQDKENYNNIQTLESRLFIYSEKLKELNQQVQTLQKDVVKLEIQKSLVEKKLEDTTLQKDQEIQKLKQEIDDIQLQNQKLQSEITTRSKVLSIHSQSSQHLLAQELQESHLKIQNQEKNTDGIQTNAVQIAQIEEQFLAEKEQYHQIIYNLMADIKKQEIMYNDLKKENNQNQQNYQHLLKIKYETGNLTPKIKKLELQNELLKKIVDAFIQTNSQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VTK3</accession>
    <name>A0A8S1VTK3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CDT1 Geminin-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0720105</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000072">
        <property type="protein sequence ID" value="CAD8179563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VTK3"/>
    <dbReference type="OrthoDB" id="284032at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014939">
        <property type="entry name" value="CDT1_Gemini-bd-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08839">
        <property type="entry name" value="CDT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CDT1 Geminin-binding" evidence="2">
        <location>
            <begin position="142"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="144"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8179563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="315" mass="37337" checksum="94CCD7EF4D8E39F7" modified="2022-10-12" version="1">MQDFLFISDNIQPTIKKVKLDLFNSFEDETTSTIIKKQDPSFIPFLSLRQKFGVHQKGGYYPLPKKYVSLLDLCNKIEMNYLRNQCQIQFTIQNNFPEIIQVLQLLNLDPKLYKIKVIQDGIVIQHPFQINSNKSDNITIVKSSQEIAQLYDLINKRIQQLREKLDQQVQIKHKEHLSIINCEDFEQFGSQFKIYHNDFNLNDCPDIKPIKLKKTIFSNELIQFHLDMLNFMTEHYDKRGVSFMFYDHIIIILSVHFNKVKSEIEKALKNIINLIPDKIQILMNHINKQRWMIQLDRASLTQQNKKCILNSFLCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VW62</accession>
    <name>A0A8S1VW62_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA/RNA-binding protein Alba-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0750186</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000075">
        <property type="protein sequence ID" value="CAD8181071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VW62"/>
    <dbReference type="OrthoDB" id="1699369at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014560">
        <property type="entry name" value="UCP030333_Alba"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31947">
        <property type="entry name" value="DNA/RNA-BINDING PROTEIN ALBA 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31947:SF36">
        <property type="entry name" value="DNA_RNA-BINDING PROTEIN ALBA-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/RNA-binding protein Alba-like" evidence="1">
        <location>
            <begin position="17"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8181071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="106" mass="11967" checksum="14736754926830A5" modified="2022-10-12" version="1">MDQQKKTLIDPKDKTFQLRGSAKPRTSVFISKIFLKKFDEVDIHALGDAISGAVRCAETLQRQGLATIQKIETLTQTLENGKKQKIIVSLKVTQDGKKRINEEIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W271</accession>
    <name>A0A8S1W271_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tyrosine-protein phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0820141</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000082">
        <property type="protein sequence ID" value="CAD8184104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W271"/>
    <dbReference type="OrthoDB" id="10252009at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46653">
        <property type="entry name" value="SPECIFICITY PROTEIN PHOSPHATASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46653:SF1">
        <property type="entry name" value="SPECIFICITY PROTEIN PHOSPHATASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="2">
        <location>
            <begin position="8"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="3">
        <location>
            <begin position="75"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="330"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="494"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="634"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="753"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="337"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="370"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="494"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="518"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="531"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="634"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="753"/>
            <end position="768"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8184104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="791" mass="90985" checksum="E10C5F31D4B54756" modified="2022-10-12" version="1">MAQSIIVGAIKIKDGLFIGDEYASQDREFIVNNKVTHIINCSGTEVQNKWNTMNIKYLTFNWLEQDNEVLFDEKNENVNKIYAFIEECFQQGESCLVHSLRGQSRACCVLAAYFMKKYSWTLYKTLEYLNSRRPDLEIRASFFYQLNALESKMNKSGPKRTASWNELTQDEKNPQQLQEELIIRNTFLNSQNGPVDDIYTNLQSKLHVLGKVYNQKIKWKDQINKENIQLATLVYCGSPDFVPTSEIILKEDSDCQSILRGVQKTSLKQPAKQQQIQHNIQKCSNQHKQTIKQLQQEDCDSFKQNAQMSNNQSFQNLLMHCAQKQKQNQQLEKKTPLISQEQINSPQFLNQTSGLRSTSLQQNDEKKNQIDVTNNRPSSVKQKDTSPSILTNFQEFKNQVQQSLHYFNQQSEAIFNKDKHLMNVIQQQYHNNNNYLNRNQSFLASQQKTKLDQLISPTFISTMSSAKHFEIQKTLTQSISQLQSSYQKFQQLQMKNQMMKSSQSTSQIQQQNNNKNQAELSTTNITNTSKIQDRSISHIKKGEELDSKNRPNSAEIKDQYKSNSTQKKESLSPFQKDPIKKMQQFGPPLPQSNSQIYLRNVQCRRQAASTLRNQQKPNNSFQDKYLNQSANQVQNNSSFNNNSNSNIDTDSKMQQKAAQGLVSDVNHLKKLISPQCLTKSQAQFLKNQPIKVLQELTEKTQSIKQIKGKESIQSAGHTLMQKAQTGNTKTLSPAIKNDEKYKNSNVCNPQNLRQKIRNSSPGLNKDQVSSSSFSYSNTNTTLPGKNNWKML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W9N5</accession>
    <name>A0A8S1W9N5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0850210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W9N5"/>
    <dbReference type="OrthoDB" id="288122at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8185667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="310" mass="35956" checksum="D06296680B5CD6EF" modified="2022-10-12" version="1">MRSPSLFSPQKQSRYEKSNSSTGFTTFQATTQMNTKQLADYRRDIRTNVSSREPFTQPQEKSSSLFASWSKAEKVVLEQQKVNRKSPKKSKLLGGLNYEEIFTNKGPQTKHNKDKVEQLFKRSPKNDYFDNSFTSFPKPARQNTLGSGYKDSPQKSKEKLERFKNWDNRLDVQLILQNKSITNKISSTGDKPGFYKYVNEAQKGLFDNNQSNFLYSKTNKNTLFDNQKQSFSFKDTFNPPKQTTFQRNHSPQLSNRYVEKFPLTDIVRMTSHFQSLSTQEVNSIPRGYISELNNLADILQRVVKRSNNIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WAA7</accession>
    <name>A0A8S1WAA7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0850028</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WAA7"/>
    <dbReference type="OrthoDB" id="299441at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8185325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="87" mass="10531" checksum="E04638EA3BF8480B" modified="2022-10-12" version="1">MNRKMPIIEEFYFVKTKSPLIMPIAGDPCNLFNETTLDCSCSKQNREEQYLRKFKNVMIEEDQQKQKFLILQKELSLYFETLRKFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WAX2</accession>
    <name>A0A8S1WAX2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0840132</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8185039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WAX2"/>
    <dbReference type="OrthoDB" id="301398at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8185039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="160" mass="19279" checksum="FB316476DE8C12FF" modified="2022-10-12" version="1">MQNSQKSNEQMKFDSFVLKNQIDNKWQYKQKKKKRLLSKIAFGSSQERQQNIRSPQLLDYQINYLSVLPHARNVLISKNERTQRSITEISKSYHYPINVKFPTINQQHIQENIKPCLFQVKEYTPKLYDDLPYTSDQLKEFFNQLTSKHKVKKPDQYFIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WDX8</accession>
    <name>A0A8S1WDX8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ATP-grasp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0820152</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000082">
        <property type="protein sequence ID" value="CAD8184126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WDX8"/>
    <dbReference type="OrthoDB" id="283371at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013651">
        <property type="entry name" value="ATP-grasp_RimK-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621:SF0">
        <property type="entry name" value="BETA-CITRYLGLUTAMATE SYNTHASE B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 MODIFICATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08443">
        <property type="entry name" value="RimK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="2">
        <location>
            <begin position="112"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8184126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="311" mass="35658" checksum="E60650487963602C" modified="2022-10-12" version="1">MLNENPIILYMKNPDPIFYGMMDDLVIQTLKKQNATIIVTELEELSVIIGEEFIFLRNGEQIQFDAFLGYGYMAPKHYLDYMYFNFAAYTAGKTTLYSPFTENILQDKLLQYAKFSKCQVPFPRCSASFSVQQFKLNLSQFESKAIMKEVIGYEGTGVKLSCNKIQSTEIFCKSLWNGEQAIIQDFVSDSVGRSIRVLVIGGRAVSVTEFQDNIDFKSNGYSDDFKIESKMDCQKKEEYFRLAEKTCQAIDPNLTIGGVDIIDSRKHGLVVLEINQWPEITFSEDVTGLPLFDIFGQEFIRKIKKNKQDKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WFE5</accession>
    <name>A0A8S1WFE5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0890055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000089">
        <property type="protein sequence ID" value="CAD8187395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WFE5"/>
    <dbReference type="OrthoDB" id="286354at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="86"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8187395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="466" mass="54299" checksum="6C11D0DBE4142389" modified="2022-10-12" version="1">MNVYCQMAGYHQDEPLELLCLNPECISNNKVLNCIACIEENHSGHSVIHYKKFITLLQKQGSQLLNEIDFKLQDSTNMQNDMLNKIREIIKQLTELQDIVNDAINKQKEMLERKLQYYEQFACLLPTQEIFNQAIKSHDQSLLQKLMTQFFLKVDYNQKRQEFVARSNYLIDQSEETEGENILFRKLREFINSFRQDIDNNYLTQNSQLSTSFKQKKHSQINNEFKRSHQRIASYKRVVSAEKSPGSPDDRISITQSLSQFYYKPNTIKKRVGEQIQLIETQNNYTTTYSFHSECFGQGLQITSKQVKLTTDNEKRIALIKPSIYGSGLAREQRITLLIKVCRNSNQRYPMAVGICDQSKLQEDNFIFSGSAEHVPGSHNKDHGCYLLNANGYIRSMIGLDMSKSSPNLRFYSNSTLELTFKPFHKQLIINRDQQVQTTIPLEFHKDQKLFFCVRLADLNDCIEIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WG20</accession>
    <name>A0A8S1WG20_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0910056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000091">
        <property type="protein sequence ID" value="CAD8188273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WG20"/>
    <dbReference type="OrthoDB" id="301578at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="280"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8188273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="312" mass="36906" checksum="A5775347E3EF2038" modified="2022-10-12" version="1">MVKQQKKSNITVIPPQPDFQEQEIELLQEDLNDDLIKTLSKANTNSIATIKEKEKKVVEFKSRVLELVEIFVSNTKNPSIFIERIYPFIKMISDFKNPQKIQYINRVCKLIHQIIQKGDLGEHIDNMKQCCETLITYLHKCQDKSINKRFFELLDLILKQISQHDKKAVSDIIVKGLVLMRKNSNIQMQNIRKLIFNYQFTLIPVILKLCQLSEINENYERSKYNFSHLLFTLIQNEQIKKKIISKDKIKQAIVKLAQHTLENYAQQNYKRVAIFRQILLSYKSLELNELQEQIQTFQSEDQNIQHIITKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WGF3</accession>
    <name>A0A8S1WGF3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0890202</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000089">
        <property type="protein sequence ID" value="CAD8187680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WGF3"/>
    <dbReference type="OrthoDB" id="305942at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="108"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="302"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="119"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8187680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="622" mass="74783" checksum="D669E57C15272A9B" modified="2022-10-12" version="1">MNKQKLAQEKQYRDQAKYRTLFSQEPQNPFGEKDVEKMKQQYAQILKEQILDSQRHKEQQKTIDRQETQKQIQYESPFGKDRLIHLQKLNRELLKPIQDIDEFVKKKNQTDISRPKTILKNQSTERSNQQYSNDNSVEQVPQYNFDSPNQQENKEYWQNRNQNQILPTQQTQRIRDYQSSQSLGLPEPILQNNSNLLMKNSKNINFPIQLPPKTPGIVYDKQNNPILSDYVSIYDQQFSRLHKSEKSDFMKELNQLRSQFFVQQKPQVQPFKQMANLQQSPPKFTQDVGIQDDQYRQELMERERQEEQIRQQEYLRQLEEQKKREQEEYEKQLREKIERELEEQRRLKQQEIEIQTEKLVTDTGIQHVDQNAVTAAEYMKTLKSLSGSVIDMKNQFIQGQQKLNYELIQLRDKAQRDIEEKLRIQKELKELEQRNQAMKVWEGEKQRQLMQALEKNAPIPQYKSAYMDPKQINPTVKKVIRFKDQTQGYEYEEPNGVMSKSNLMPLNGGYDQKHLHNLKANEPVLPEELEVFRQEVAPQLKYSNPITRQVRMDDLNDQFYDYKYQRQLEHPGQDRIERSKYETQIKNDPNQVLLENDRRLHELRIIDPRGDNKQIQELYDYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WGP4</accession>
    <name>A0A8S1WGP4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0890012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000089">
        <property type="protein sequence ID" value="CAD8187319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WGP4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8187319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="155" mass="19150" checksum="A5C32F9B96484088" modified="2022-10-12" version="1">MYYSDYVIIIEYFLIQIGNQSQFLQLIIFKIFSQQLYSCISYNSIFGSIMESNNLRLEIMVLKDQEMYPKNCKYYKNFIYLRNYCQCSSRKYCLFNYLFLRKLKEPDESSFCTYFHHQQQANQQKRARSIKIEVQMNFLKYMQFLLYLDRIWRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WHD2</accession>
    <name>A0A8S1WHD2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0910111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000091">
        <property type="protein sequence ID" value="CAD8188381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WHD2"/>
    <dbReference type="OrthoDB" id="285012at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8188381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="149" mass="17484" checksum="B78B49A36034E5DA" modified="2022-10-12" version="1">MNLEQIQKELDSINRQTKIINLQCKLYTAYHSFMYRFMNKDNFKVYTFRNHYGSYMALGMAIHNTYHLKQSILKQCIHLTKSKNPTIIKLAAIPITTVYFGVLFGLWHIPSFTGELAIRIYFGMQSAVFDFLVDKESTFQDIPLRSIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WIE0</accession>
    <name>A0A8S1WIE0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TROVE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0910165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000091">
        <property type="protein sequence ID" value="CAD8188485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WIE0"/>
    <dbReference type="OrthoDB" id="427368at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005697">
        <property type="term" value="C:telomerase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003720">
        <property type="term" value="F:telomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070034">
        <property type="term" value="F:telomerase RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000722">
        <property type="term" value="P:telomere maintenance via recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052652">
        <property type="entry name" value="Telomerase_Complex_Comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008858">
        <property type="entry name" value="TROVE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44791:SF1">
        <property type="entry name" value="TELOMERASE PROTEIN COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44791">
        <property type="entry name" value="TELOMERASE PROTEIN COMPONENT 1 TEP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05731">
        <property type="entry name" value="TROVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50988">
        <property type="entry name" value="TROVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TROVE" evidence="1">
        <location>
            <begin position="8"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8188485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="561" mass="66195" checksum="B98799169632B523" modified="2022-10-12" version="1">MIPKQFQFFLDLTCCQQRNDPKEYFFSITACCLLKETKFWETSDMSQDKLIATMNIIADIDPEFIFQVAYYVRNQMYIRSVSNFILAFSALHPKTKPYCSTYMCPTMLIPGDLIEVCQFVQLISNKLKSNNQLNGSTNIRKQLFFPTILQKQFRKKLTQLNIYQLGKQCSDCSRKKNIKIYQQILNPDIKNKKRENRLLNLKQIREVLKYQGKDVQEQFEKRTIQRTNQRRKNMRNHKNDYIIQENLTVFDINFLHLKDIIKFSHVKEPRSLVMSILGAKYPKTQEEFEKEFKGEVKLKFDPEKAGQRMQIPIPITWDRELSKGLNSKRQNLEDLIQKNQIPYLALLRSLRNILKQGLSAEAHQKVVQKLSNKQCVENAKIFPLQFFTAFNEIDKWQTNQVKNQEPKKERAKPYEKQKQVKIEEKEEEVTVDPQLIESYKDTIEKAIKIVVDKNLDTIFGVTYVFFDVSASMRSPISGGKKYGSINSCMDCAFVLAHLIRIKCENCQFYLFSSPYRTEHPYTKVNFDDDTLFEGVKDKAKSYNKIINYLYMINHSIQFLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WJA0</accession>
    <name>A0A8S1WJA0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0940069</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WJA0"/>
    <dbReference type="OrthoDB" id="346907at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000654">
        <property type="entry name" value="Protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="14"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8189523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="589" mass="69049" checksum="E59C1A8DB368B796" modified="2022-10-12" version="1">MNEDWTTKKVDKYVVVNKTLGQGAYGIVYRGFLQEDETKQVAVKTIKIATISDTPKMIELIKREIAILQKINHPNIVRLFDVARTNNYLYMFLEYCADGDLKDYMAKKEEKRLSELEAVIFIKHIVEGFKRLYKQKIIHRDIKPANILLHQGVAKITDFGFARVMDSEMNDPAYLSRLGSPLYMAPQILEGQPFSAKCDVWSVGVMFYELLYGRTPWSAENAYSLLENIKKQSLKFPPKPVRSQKIKELITLMLRVQEKDRISWEGVFEDPTIKIDEETIKENMKNILKEKDEISKSISLNKLYIDQNLVVGYLAKNPINTLENSNQQTLEASAEARSQSAKFSSQFDSDSNADIIMSNYKNEKKRRDAMLKYNNYFLFERNIAFFFNFVIQKIIKLQTKLQLANDNYFRLIYLIAKNQMVHLERVNQQLQRKDHDKFDKETWGRFLASQEYQKLIQLISMDMKHSLDFYTEISKKCNIVQQEELANGKATGAVQSLIKNFLSIQNNNFEANDTFHMLYRDIIVENLKIIKNLNSKETEINLLLLYLLICQNPYEEFKDINYDFNTFYEEIENLTLQEVQEKLQKKGQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WKW7</accession>
    <name>A0A8S1WKW7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN motif protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0930143</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000093">
        <property type="protein sequence ID" value="CAD8189320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WKW7"/>
    <dbReference type="OrthoDB" id="287158at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF263">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="407"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="411"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8189320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="426" mass="49134" checksum="5738DF1A93AA7416" modified="2022-10-12" version="1">MIDNQTLQSILNYQNVTEGYSYGELSQLKNFKTVLSNDKIYFGQVFNSKKHGKGVLWSKTGHIYEGEFNNNRKHGFGMELFQQKSSYCGSYLNGRPEGLGKFIWGNGESYDGEWLNGYKHGYGTWIGLKGDSYSGQWAYDKPHGQGIHKWINGEQYQGEFKECLKHGFGEEIFSNGDRYIGMYQNGTPEGEGEYFYSSGAYFHGQFLNGLKSGYGEYRCANYSYKGYYQNDKKNGEGELIYADGSRRKGKFYNDLFENNETTKLTINTQPENSPQNLYNITNFKTPFYLSQTKPIQKELNSSSINKTVNITRNIQESKFQKQLNLLPIMNNKLRSNSTKQSNQDSERILYINQYNNNKSVNSKQNSRKTSVVTQPIAITQTIYRSKNQQDFSQKLESTIRKFHYKTELKGTKNQNHRSVSSSKQKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WPT4</accession>
    <name>A0A8S1WPT4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA polymerase delta small subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0980148</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase delta/II small subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000098">
        <property type="protein sequence ID" value="CAD8191232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WPT4"/>
    <dbReference type="OrthoDB" id="3763at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043625">
        <property type="term" value="C:delta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07387">
        <property type="entry name" value="MPP_PolD2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.50:FF:000011">
        <property type="entry name" value="DNA polymerase delta subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.430:FF:000008">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007185">
        <property type="entry name" value="DNA_pol_a/d/e_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040663">
        <property type="entry name" value="DNA_pol_D_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024826">
        <property type="entry name" value="DNA_pol_delta/II_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041863">
        <property type="entry name" value="PolD2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416:SF0">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18018">
        <property type="entry name" value="DNA_pol_D_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04042">
        <property type="entry name" value="DNA_pol_E_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA polymerase delta subunit OB-fold" evidence="6">
        <location>
            <begin position="51"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase alpha/delta/epsilon subunit B" evidence="5">
        <location>
            <begin position="203"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF18018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8191232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="454" mass="52042" checksum="0B883E406496843B" modified="2022-10-12" version="1">MEVETNTDMSFILQKLKINSEKKFKHQRIGLEIKQSIKFYLSPKNKQIKTQYSHIYYMRYLNLAKRFQINSEDSQFIELIQKIQNQQGTIKAIGTVFKEMKLKPYYFCQNQNKSLQDYVSNEDVCYLEDRSGRVKLQINNALLCLPNKKDKIINVSDLVTGITLMIEGQIVANNIVKVEKFYLPILPETPMLKQLNSTSYLCLISGLNYNALESTTKYRHMIDYLQGNLYSGEGSEIPYQISQVIFLGNLYCKLEETTDQQILQQDFKGLYNKLQLNIKGVDELISQLISVTPVAIMPGVNEPVSQMLPQTPLHKSHFPETFDKSNQLVFLTNPTEFTLGDIKILGTSGQNIQDIKKYSLVKNQEDVDILEMTLYYGHIAPTAPDTLISFPQQDQDPFVLQELPNIYFAGNMQKFGTKMVTDNVRIVSVPAFSETGTICLINLNTLECFPVHIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WQ86</accession>
    <name>A0A8S1WQ86_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0970194</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000097">
        <property type="protein sequence ID" value="CAD8190940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WQ86"/>
    <dbReference type="OrthoDB" id="674604at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="33"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8190940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="254" mass="30162" checksum="E3124067CB6D7418" modified="2022-10-12" version="1">MMKSDHFISGSYDKLIIIWKRNQNNQWICQQKLNGHTNAIFCLVLNNNEDLIASGGQDNTINLWQKQNEWFCSQTIKDHTKRVLGLSFNEEQNRLISCGQDYCILIIEQSEQNKQWNVIQKIKVKNYQGVRLCFIDNNTFTLLYPWGRDYMHVFELNTSNKQYSNTKDIPLKIKSGFENTQFPQQYIKSKCIIVNKNASNVNLIRKQQNGEFTIEQSIQFEKYQIFGYMTDDGQYLITWDAQSKEYQIRKYQEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WSD2</accession>
    <name>A0A8S1WSD2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PSI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1030022</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000103">
        <property type="protein sequence ID" value="CAD8192934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="430340at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002895">
        <property type="entry name" value="Paramecium_SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01508">
        <property type="entry name" value="Paramecium_SA"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SMART" id="SM00639">
        <property type="entry name" value="PSA"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035751590" description="PSI domain-containing protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="1934"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="124"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="661"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="1557"/>
            <end position="1602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8192934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1934" mass="205127" checksum="BCC58DE8C7697C7F" modified="2022-10-12" version="1">MRVQMIIGILLLSASATDVDVSNQCTCEKLLSEADCKSLKGCNWFATTSICSKLDLGANSTEGYCGLISDATTCAKTKGCAYVDSICQIFAGCTAYKGATDAECQAISKYCNSDGQAFCVDPGLCTTYKTSEDCISKASSGGSGTCVWDQSCRDQKCSEADPTFNSDAQCNNFIEKCVTTGKGCINNLGLCSSYSGGIESCEGLIGSDGYCKGVDGKDKCEVKACTDADTTLTTDAACAKYQIGCVTNGYGCVKSPLGLCSTQTGDDVICANKIGSDGKCKGTTEGKNCSAVECKDAPETYSTNDECKKFKIGCVTTGKGCISQLGNCSSYKGTSITCDGYVGIDGKCKGTSDTEAPCSVKVCKDAEATLDTDEKCEEYQTGCKTTGKGCVQTLSNCSSYTGDSTSCDGYIGIDGKCTGGTSSGSCYPKKCSDAPSTLITNQECSDFQTFCISTGFGGCMDVGTCSSLSNKSQCDGKSKCSWSPQCVKNVICSDLKSESLCNTVSLQDNKTCWWVDSNCVERTCNKAPTTYDSDLKCDTFLKGCLTNKKGCVSQTATLCSDKTNGTTDEACKSFHYGCVTNGTACVSISAACDTYLGTSTEICSKFKGNNQQKPCWWKSGPKCVDRQCIDADDTYTTDDKCEQFLKGCITTGAGCVQSSDDCESYTELNQASCLTLSTGCARREKCTVELTCAQITNATSQINCDDILPKQCIFIKNACVPLGNCNTYTGTSIDECKDLFNLNGNKCYWSTSNKCSDRECTQIKSPQNLKACTDHKSSCAIKTGGSECQEFACNLAGTPNPATEETCQLYHPECHLGYVSGQAVCTLPLACNGYGPSNTASCSTLIALKGQTCKSGQNQTDNCQPAECSNFHTKETECKSAKIALAANNLKPGTGQYQYCFYDSTSQCVERSCNNAKQHLGGTVTSFDQCNKYFEQCVLSKPSAGSICVDGYVDTNNFGCKKLEGTQDDCLILQGKYKLSENVYEYRLCQKHDQEGVANCLDRTCDQKTDGYTDAICQTWLPTCKTNGVSCVDISTSCTTLEGTSDSCKLLTGLVFGSKCQGNTSVKGVCVARRCQDNGTAKTDADCKKFLKGCITTGNGCVDENTPCSALQGNQDLCKLLTGNSKPCWSLSLTTKGACIDRTCSHNTTAKTNAECDTFMKGCVTKNPGCIETTAECTSYQGIQADCEKYIGNGKTCTNDATATNTTTCKVKDCTKLSADTYSQSVCQAYGVQCHYNGSKCDVAQSCSQLKGNLVTCTQYVATDGPCIGTALQTETPTSCSTAKCADAPSTLVTDAQCETYKKGCKTTGQGCASSITCGDVQSSSICSAVKIDDDFICTWSSQCRDIESSCSNFTSSGSSVCTSSKTTSGFGICIWKDNVCTEAKCEDLPLSVSSETDCIAYSTTCTFGGTGNGCATKGECTTYKKKEICANAKSTDTMGSCAWDENIVTGTQIKGCRVKECQDAATTLISDSDCNSFIEGCVSNGAGCMKPIFTCDMLKSQTKCIKDSQLRPCLWINNACRQYYNCTDLSLTTVAACQAVSKYCTTDENKCTPLKICANYTKQSQCTIGTDGFCGWVTNTKKCQLFTQCSDLVSKINTECQSYNPKCISNETTCVEKGECSTYADSEAACTIGGTDGTCYFDKDTTSCRLRQCSDASTSTSTHNGCFSYQINTTSKCTTNESKCIPLADCSSYTKQGGCVIDSQLKACTWDNMAKACKSKTCSDTKKTKSSECASALAGCISDGTKCIEKGKCSDYSTKEACSAGGTDGACVFSPTSSSATTGVCKLYTQCSDANSDQIACQSMSQTCKWTVATGTTPSSCSNHTCDTAAKGTAKCVTIPSFDGKKYTVCQPQGGKCVVGDPSSLTQDNCYKMSQYTYSWNAQTKKCIACGAGSTSNNNSTSVNNSMDNTSNTTTDDHGQYLYVLPILISFIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WVY6</accession>
    <name>A0A8S1WVY6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1010154</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000101">
        <property type="protein sequence ID" value="CAD8192435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WVY6"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8192435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="104" mass="12351" checksum="03CBEEC36F4F3067" modified="2022-10-12" version="1">MHKKISLCNGQFKTIRKEQLQCLEHHPQSLFKNIINKLQNHIFSSTAQPQILKKPMFIFQKTILKVKLNHIFGYLQIAFLFLFTDLLDQNMRKVKILPIFIKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WXC9</accession>
    <name>A0A8S1WXC9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1060015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WXC9"/>
    <dbReference type="OrthoDB" id="303435at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="427"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="432"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8194082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="544" mass="63722" checksum="EEAB16608A5C10E7" modified="2022-10-12" version="1">MNLAISSSSEEESSYNAKEQDRILKNSNIFKQKANSHLNCLKHPNKKAKYYAQLDKRTLFCSKCALNLALKGHKIEESAGTELEFQRQMRINQFQELMKQTLQACNFKIIDFNNLQISQKQLLEDQKANCIRFFEQVIETAQQLKQTYIQKFQHDHSILEQNILDCQQAVTNYEKQLQQFQIDIEKNHSNIVKKMEMKPFEDIMQRYEKRVKNIQQLLSEMKLDNSVKINQFEQSQILTFMNKMCYNILLNDTSQSIKSRSNSINNNNNPSIDMKAFDILENDDIYQSTFSNTIKDNNSQNPPSPISKLPIYKNNGSNPISTIQNSRRESNSNTPESWNHTNIQNCDQQSQIALQANSHNVSLIEQPILINQQQQQHCVQDIVKQTNDNPQIQSSEQQKNLEITPLIYQKSQSILPQSIKIYNSQRNQYDSNNNNNNNNNNNNNNNNNKSQDQKQRKKQTSVDYFEQKRFYIINQNQQKPQQQQQEDSLKDRIFKELSGHSSESVYNQVLKINNTQQKIKKGKENTQIDWTGSLKYKQNLLIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X4G4</accession>
    <name>A0A8S1X4G4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Dynein heavy chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T1090119</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000109">
        <property type="protein sequence ID" value="CAD8195439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X4G4"/>
    <dbReference type="OrthoDB" id="292742at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.490.20:FF:000005">
        <property type="entry name" value="Dynein axonemal heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.280:FF:000030">
        <property type="entry name" value="Dynein heavy chain 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.720:FF:000001">
        <property type="entry name" value="dynein heavy chain 7, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878:SF68">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein heavy chain AAA lid" evidence="9">
        <location>
            <begin position="2"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain C-terminal" evidence="10">
        <location>
            <begin position="141"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8195439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="452" mass="51649" checksum="6BE883A0CB0B296D" modified="2022-10-12" version="1">MGLCYFHAIIQERKKFGPLGWNIKYEFNDSDLETSKTVIKMLLGENDTVPWDAMLYVSGNINYGGRVTDDWDRRCLMTILRKFICNEVLDDNYVFCENNTYRIPEKSVVEDYIKYVESLPMTDDPAVFGMHENANITFQLRESDSILETILSIQPREGGGSSEKTPDQIVLELVKSIQDDLPPLLSKEESNKELWQINPEKNLIPSLSTVLLQELERFNILLSTMARTLQGLAQAIEGIVVMSQELDSMYYSLMNNEVPKVWNKVGYLSLKGLASWIRDLKERVKFMSEWLITGGPNSFWISGFFYPQGFLTGVLQTHARKTAIAIDNLVFSFKVQEFEKEQCSIKPVDGVFIYGLFLEGAQWKKKCLADLNFGQMSVLMPVIHFLPLQQDKYQQRSDNYSCPVYKTQTRAGVLSTTGQSTNYVLAVDLPTLDQPPDYWTLRGTALICALKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X4W3</accession>
    <name>A0A8S1X4W3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1110143</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000111">
        <property type="protein sequence ID" value="CAD8196127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X4W3"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000334">
        <property type="entry name" value="Heat shock factor protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF206">
        <property type="entry name" value="HSF-TYPE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="3">
        <location>
            <begin position="9"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8196127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="267" mass="31495" checksum="AF2E35C7C13F968C" modified="2022-10-12" version="1">MSKSRSQSSIPAFLQKTYDILENSQLLDIVGWNEDGSGFLVKNVIAFQDQVLPMYFKHRNFASFVRQMNMYGFHKSRSDQKENEFIHPHFKKDQRNLLKKIKRKSGEHGDDQFTIMELKPHRNQNISDKQIQQIMTKQQELEKVCKILIEQNNKILQCNQQLRNQLVQEKFNGNKKIQKLKDYFICQQQIQGDCLQKRSLEDLISSDNEELILPNIKKIKGEDSDSTFDKMEQLYGQPPLMLTTTLVENDGMIQLLDDHQFNDIYFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X6E1</accession>
    <name>A0A8S1X6E1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1060072</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X6E1"/>
    <dbReference type="OrthoDB" id="301928at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8194196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="92" mass="10537" checksum="30641CA29ABF1841" modified="2022-10-12" version="1">MGCAQVVNHSNDIQEPIFERKSSKRTTSKQNTSCQDSPIIFPLNLPDGFGKYKKNRQNTMSKTKLRNAMRIEIQLDTSKNTILQRRTISPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X7J6</accession>
    <name>A0A8S1X7J6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1140100</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000114">
        <property type="protein sequence ID" value="CAD8197075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X7J6"/>
    <dbReference type="OrthoDB" id="421226at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="300"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="351"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="379"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="3">
        <location>
            <begin position="524"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8197075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="913" mass="108581" checksum="50CAE53023F56B48" modified="2022-10-12" version="1">MNFDQINSEPDEPNLDTIQNKEKFKQSPKELIKKRNKRNALSDYNKLQQFNEQLIVERQIQNRGSMLSFFLKNIYVKRFLNRLLLRKKIKQQYQQEIFNLIDDKSAYYTTISNRIQQFPSLLNLDFFQNVTNLMEKKVYFVIKKYISIIPIIQPENYAKITFDVIGTTARLYFLYLIPVDLAWAQYAFLFQSLSFITIIMITILIAEFLFNLNVAYYQEGNMVTDRKQIAKNLISKSYGLEIFSTIILILALIFPDIQTQKQDSTPQNLIFLLFLVHKVNIDRIVTVYETAINLSKKFSSYLTLVKLLMFFFYVVHVFACLWYWVGKYNKNNPENWLYRAEILDDSWDQQYLVSFYYACVTMFTVGYGDLTPKTQIERVVTILFIILSSVQLPYSVNTVGNIIQQISAYGEERKNKLRTINSYMQRKNVPYTLQNQIRQYLDYYWQLQASEESEDEKQIIQQLPENLRDTLMIKANHSFFNKVSLFKDNFSIAFKQKLLKKINHLVLQPGQIIEIPDEKQDCPTLFYIEEGTIEVFLNQKHKQCIRKVNKQQALGIDNFIVGTITTEIFKSIGLSKLLILQRNDFINVLSQFPEDFETFCKLKDDILFNQDLQLFEYNCIACGMNSHKVVHCPFIHYAPQKDFIIKKFNYYKNQKRRDHLRSNNKTKRYFQLSELNFLKKQISIISNKITTPHLVQNKQQQKSLTQSKNLLSNPHVKSNSQQKPLDMQPLRLIDYGEKPMIIYNLRGNTQNTCRINQKSNTKILFINAVKKVIAIQRWFGRNDLSMIDRFETSFDCKLIRELIVDLKRAKEVNQKDLDSIEMLLIKTKNLNKYERKRMDQFELAREFKVYFSSFNLSKIVERLNFTKYQKYYIRKLQKLLPYLKYPGNFIKQYKVKLKHKKLKRSHNNNVLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XFE6</accession>
    <name>A0A8S1XFE6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UvrD-like helicase ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1230019</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000123">
        <property type="protein sequence ID" value="CAD8199930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="3156807at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039904">
        <property type="entry name" value="TRANK1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21529">
        <property type="entry name" value="MAMMARY TURMOR VIRUS RECEPTOR HOMOLOG 1, 2 MTVR1, 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21529:SF4">
        <property type="entry name" value="TPR AND ANKYRIN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8199930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2238" mass="266994" checksum="8ACBCC656770B445" modified="2022-10-12" version="1">MNNNQYKEDQLKLYHENLNQYQSKLHLIYEQFNQKTQQFQYFHQFIIHFLSQYSKEIKYKEQSIQAVNINQIFPNRLWEFDITIKFIRNMTNIYNIQLLMIMSYLLQGRFNEENKEFTNILNLNCCSLKLPFQYAFSASLVSLQSLLIIVLILPQKQYITNSLDELVEIQFIHKMCFIDIIQDSYRNNADKIKKCQTILDEYLHQYLKGNDLLSNIKQIKEKQKSCQSSTKIKIGIKTKNKNLLIQPRSFETIKLNSHLYIFDNQSNTWMTKKLYESIQKKNMKINAQNENYYLIKEYICLPSAIQISKLIQKLPKFKNKISEQQLQAIVWQGITFILGRSGTGKTTCALLKLFILDALFNLRQELKIQNYIIKSQIKQQIQSQFIPKDKLTLKTLFITASPLLAWQIKQNYLQLIDNFQELIKEKFSNINQSHNIEEESLYEIFNELQDSQIKENNTDTDEEEIDDYEKMMGRFQKLSEITEYPAFLTLRKLIFSIDASLLNPYFKFSQTQHCSAQWHNEQIGIVSLNQQSQYNNEKLQMKIKDYDDKEFIVYNNTQEVTFDLFLNFIWPKIIKKLEHSNQKIKQLDPALVWYEIMSKIKGHATSYEYPNKYMNYDNYSYYHKVLSDEYTKLLYKAFEHYELIKQNLGYYDLLDVVNHINYELKYGNDILENVHYLILDELQDIPNAIFILLNQIADFGLICCGDNAQNIQKGTGQQFVEFRNLLNESNLKKKYRNNYISTFKLFQNFRFHDQILQLTNSIIRMIELLFPYKIDVFDKEERSYLQGPKPIVIQSEDIQILFNYLKKNSKIESNYVSFGSNQVIIVRDQESKPKVPNSLQQTLILTIYEAKGLEFDDVILFNFFTDSDSTCDDWNILKNFQIKDVFIKMQQNPNIFIYHEFYETIQMKKLFLIQNTQQQNISDNIDRFVNYQTICQELKLLYVALTRAKRQIIIYDQNYSKRKTIQKLWDDLKLIEIIYTSQIENVQEFEILFSQQFDNKNSWRNQGLNFFRVNNYEQAKKCFKFAKEYQLEQKAQAYQLATQATLIDKGENLFYEAALIFEDLNIKNRAAQCYFSAKKYKDAYRLYKQLNSKMEIAEAAYFCKEYEEAGQLFLEVKDIRRSIESYIQQGNYKKVIELIIKYKDDLSQEEYQLYLNKYFPIVLQEIFNQNELQNEFLLYDEQSESFSVCNSDLLQSCEKLEFQLKNSKLSRNENKSSLSESFQVINNDSFDHLSIYDPDDEWIQNDKVQLITSIASIDVRSSNDNKILLLNKVDGSNLIKNKKKKVTFNESTLFQIIQLLIQISEDFKIHIQEKLGQQQLDYSHIIKLTQIDTIKFILNLLEKFQNYQLCIYICNQFNLLDQLGKYLVILASKYTPLYKNSIGVDIQMIRNTLQRKHLLDQASIAHQALQNIFEAMNSEILNFKYEDYLDYNNSFGLKCYQDLIGFGFWKQIIFKMNYKHSKDLCLSFNNHSDLIIILQNMEKQLNQDQKYQLIKSQYLLQIEQFFIDKTIKIIQIDQIFEITKSIAQGELINFKLINQIIYHSKLKEQNLNFEEKLRQLESVILSYLLCCGFISLQDVTLEQQIHLINIIYFCINQIKSICWNQNLIDAIQFLFKFSFPQGEIMNNYSQYVLLNIQSKLIKNIKPQKIYFADSSFEYLLIPFELLIIQIQNYFGRTNIQEFKIFDFQQDLQYNYQIPLTFIIKTIKHQQLQSVLKPYIQYNINQQNKNKQQLNFINQNSFDYDEYSILQVINQNEKRVFYEYLLKTNQRVVNLPLSISTSLINQCVMLFQLAFKCMGQNQHAYLILAINLCNFSNNLPLAIYSIKSIEDKLQNEKYLKYIEFLECQNYNITEDMVECFLDYCYQCEYNLYLDEWNDHLIRIGLKLILAQKFITTIVIPDYYLDYLNQDISNDNTLTQNTQFPQKNTEILMEYFNCLYNYIQSCNSPYYDQSGYLLLIVIILNLSTISQELQIIIQTILAPQNVKPQLKNILSLLNQSVQTRRSKLISQSLINTGYSEEKLISIQVIQNNQNDNQDIKIYQECLDNWNLHLSLAEQLKVNGQKILYFYIKYRQIIKQNSKEEIISNPIILRFIQHYQLPSSFLKCINYDQNWKNQLIQSYKLQDELLRTRQNSQTMRYLQEIRYHLESILNLQIELKMGHQIQDQLMNVQKLFREYQQVKQNEQIVQQLMLDRNREMLKIKWQKLQAGIKVKNKLTNISQKQCIKILEQDEEEENNII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XHR1</accession>
    <name>A0A8S1XHR1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T1240045</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000124">
        <property type="protein sequence ID" value="CAD8200288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XHR1"/>
    <dbReference type="OrthoDB" id="285969at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001436">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="10"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="134"/>
            <end position="135"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="8">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8200288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="360" mass="41775" checksum="8D8F6AED5EC9E037" modified="2022-10-12" version="1">MIIAESGQLYQSIKLLGSGSFGSVYLVKELNSKQEYACKIISKAHIQKYDASSMIQNEINILVSCNHKNIVRLHSYWEDCNNIYILLEYCSKGHLIDPKTPFNDDDVFQVFHQILDGVNYLHQNNIIHRDLKFENILIHEDGTLKLCDFGWAIKVDQKSVPNVMCGTIEYMPPEVVSKSSLDFKLDAWSLGVILYVLLYGKFPFQGDNQDQLIRNILFDQLIIPSRNDINEDLTNLIQALLIKNSQLRPSIEQIYLCKWMKLNMKSHNIFNHYENEQLKLKVKQKNITLKVVNNSLYSTPNKKMVNSNYVESKNDFDHKPTSFGKTHNSCRTIKSISINPSPQIRQIDKFFNFLENIQKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XJS7</accession>
    <name>A0A8S1XJS7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1260142</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000126">
        <property type="protein sequence ID" value="CAD8201089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XJS7"/>
    <dbReference type="OrthoDB" id="285626at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035743915" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="17"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8201089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="101" mass="11445" checksum="D5D26CFBA74E19A5" modified="2022-10-12" version="1">MIKFLLFITIISLVFSIQETTTHSDCLNQYCNVEVEACRKDQTCKKQISNCYQTAMDDLSPAGIQRSKAAYDLCLSTNSYAKVQNLAKCKQRNCPNKLLII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XKG9</accession>
    <name>A0A8S1XKG9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1270099</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000127">
        <property type="protein sequence ID" value="CAD8201354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XKG9"/>
    <dbReference type="OrthoDB" id="301434at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042392">
        <property type="term" value="F:sphingosine-1-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01610">
        <property type="entry name" value="PAP2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969:SF28">
        <property type="entry name" value="DIHYDROSPHINGOSINE 1-PHOSPHATE PHOSPHATASE LCB3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="123"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="286"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="323"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="353"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="8">
        <location>
            <begin position="79"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8201354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="410" mass="48146" checksum="B90BE86B76956EE2" modified="2022-10-12" version="1">MQFNSKKNAILGIILFCIYIILDLYIDETLLQYTYKLIFSIKNPLFIEFSYLIKTTSLLLCEIIFLYLYLSFNKKLDVLLLILFSCVLTGTHAFLKEIYNQGRPYMFNSDVQTLECIAELGKPSGHAICIVSYMISLYQADRFENEEEMKLPLFKSILYTIIALIVCLSRVILGVHSIGQVIYGIIFTLCMYMIYLNIVHQQIKKFIHRHIKYQSHKLTIFTAILFLVFLTLSLSADFYSRNYFAQQPDLYYKYMDAVQECRVRNGLEKLNDESGFILQRSTSHSYGYYFIIIGALLGLVIFRGVYEEGLYEVQSKFRTRLTLFIRIMIFYLMIFSVQYGMEYFEPKQYESYLIMIVVGHIILGLCVTLIYPILMYFLGLEIYGSMQCVNKSTLFIDYSHIWESDSMIQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XP40</accession>
    <name>A0A8S1XP40_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1300103</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000130">
        <property type="protein sequence ID" value="CAD8202222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XP40"/>
    <dbReference type="OrthoDB" id="293946at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8202222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="133" mass="15595" checksum="7174D79A69C22AAD" modified="2022-10-12" version="1">MKECFNRQTQIIMRRCDSLKNESKNLMQEIRCRSFSLAQNKCGNSSIKENIQLHNYLETQILSTAKLNFRSAKYVKKNAVSSYLQNPAYYNKLLSSVTKKRMSLQSIASRQFTIKQEKSIWKIVNQSDFHGFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XP98</accession>
    <name>A0A8S1XP98_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1320108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000132">
        <property type="protein sequence ID" value="CAD8202910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XP98"/>
    <dbReference type="OrthoDB" id="301607at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8202910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="304" mass="35394" checksum="59E4353DA54EEAEF" modified="2022-10-12" version="1">MKNFATNPKNIGGMKMLNDNMFLHHKALIDAKPAIKIKPPRPHVNNIKNVNPQKPKEIDLPMYKNKKEFDQVMKTFQTVIGMKKGRIDSNAPITMEFRRNISGNATKKEKFDRDQHILNLQSQYRRIASAGSMQERKKNSNDPIAHPPRFFRREGETMANIKIDKLLHHFTKEQRMLEETPNSELSQKIEALNKKKVQLSKPKQPRAQSAKLPSQKSEPSSRMKTRNADDITTEVKEIPKVSTNSEEDLEQLRQMLIRIIFHHKIFKTADLETLFARVLNQNPQIDLIRAEILFEMIREQLKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XT33</accession>
    <name>A0A8S1XT33_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1360133</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000136">
        <property type="protein sequence ID" value="CAD8204287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XT33"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035777287" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8204287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="260" mass="30579" checksum="21A6682E64FE3DCD" modified="2022-10-12" version="1">MLYLKTLKILGFFLQLLFQHQEQKLLLKRINISQKKCLLLLNIYKIVQQAHSCCKFKVSQTLYKSIISIQSNPLVEFNHIEQILIREVTFIGNILLKITLGIFFSLIEIYLKKTQEITMENIQYQGNIFDLYETDPIDNSASLARIESGESKIRTNNILCLTKLQQIHLIHLFPSFQMKIQLKKFQTFNHNYLDTKLWEKYYQLQIHSQFIQDQITYLIKNAYKIDTIEEALSITASQIQFKNGLFDNIIASRGLICEMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XV63</accession>
    <name>A0A8S1XV63_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1390099</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000139">
        <property type="protein sequence ID" value="CAD8205025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XV63"/>
    <dbReference type="OrthoDB" id="10339812at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8205025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="132" mass="15453" checksum="E0430A3913EBC6BF" modified="2022-10-12" version="1">MKEHQGLMTSEFKPIKQKYFLHEKEQLKQDPFVQTFFFLALMIPSIILQADYIELDVSEKGGIFTMEQTWDPPISIQMFSKTDILLLDVQLSLNGKEIEEFTIYERLYMNTQYNLTSNTNYSFSSSQIDGTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XVG3</accession>
    <name>A0A8S1XVG3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1350105</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000135">
        <property type="protein sequence ID" value="CAD8203934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XVG3"/>
    <dbReference type="OrthoDB" id="289189at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8203934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="222" mass="26481" checksum="2A99FBBDF33C4A18" modified="2022-10-12" version="1">MKSVTPVVSSNTIDVVENTISPHKTNRLSNREKRSMSNKIKDLTYDNQLFDKVDNKIRAYLEKNQVNYEKTLVKLYKLDSLKSEKAKRMLGELNQTKRKQQLSAVQQTKSIERNNSLPSSKQMQMDLKQIQQELKKINPEIEHKILMNISTFKFNQSNCARLEEEEWERVALYYPYKIRPKMTNFEQELDRLNSYYSRNENSLNQLMQIQKDLLHYKLLQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XWM3</accession>
    <name>A0A8S1XWM3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EML-like second beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1410144</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000141">
        <property type="protein sequence ID" value="CAD8205763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XWM3"/>
    <dbReference type="OrthoDB" id="674604at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002141">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="235"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="277"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="319"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="329"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="361"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="403"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="445"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="487"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="504"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="529"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="571"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="613"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="655"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="697"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8205763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="814" mass="89850" checksum="DFB45751B5CC77A8" modified="2022-10-12" version="1">MGCSFLKQGVLVQKTQLLSFPKLFPTGADIFDEVKDVDKKLFGVILEILRKEKVKDCIGFLSDIRNRRQLESQILQQAGNFTKTDTQQKLSVVRKDMKLIIDVLQKLKDHDLNQKDFSSEENQESTLNLIKSINDNKQIIEFLEFLVVLTSLDDTLIQCGSNSLHSLVEMRVDLRNKNFEKIKIKNISLEGANFVGCNFSESQLNNVNIGGINLNGTQLFNCKWRDLKIPELKKLNGHIYDIYSVCFSPDGITIASGSSDNSIRLWDVKTGQQKAKLDGHSFGIYSVCFSPDGNTLASGSHDYSICLWDTKTKKKKAKLIVHTNTVNSVCFSPDGNTLASGSDDRSIRLWDVKTGQQKAKLDGHSFGIYSVCFSPDGITIASGSSDKSIRLWDVKTGQQKAKLDGHSFGIYSVCFSPDGNTLASGSHDQSIRLWDVKKGQQKAKLDGHTSTVFSVCFSRDGKQLASGSADKSIRLWDVKTGQQIVKLDGHSHIVFSVCFSSCGKKLASGSVDRSIRLWDVKTGQQKAKLDGQTGYVYSVCFSPDGNTIASGSSDNSIRLWDVKTGQQKAKLDGHSYDIYSVCFSPDGNTLASGSDDSSISLWDVKTGQQKAKLDGHTHYVNSVCFSPDGNTLASGSDDKSIRLWDVKTGQQKVKLDGHRDTVRSVCISPDGNTLASSSNDKSIRLWDVKTGQQKGKQDGHGNCINSVCFSPDGNTLISGSWDNSFCLWDVKTGQVIKSSDKNYKDILAQFKAPIFQNNPQPFSNYSILVISQEIILEASGTLILQGEFINHQGKDMKPLFKSKGSCFLEDLKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XY07</accession>
    <name>A0A8S1XY07_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1430128</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XY07"/>
    <dbReference type="OrthoDB" id="310722at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8206296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="473" mass="54626" checksum="8C88E8664E8CAD9D" modified="2022-10-12" version="1">MIRVQTLPPEQIQQFDIEINFHYIRDLVEKCTQKLLKENVKLYYDEQCNYEINSDQSLQEARINQVIYVKVLENQSESISQYGDVICWFRQDGIDLEQGVQLRSDFTFEEVLDDAISKNIIQQMPKYIKVTNHGQTKILATLIEMQMYKLIEQNNKAPQLTIHFTTNDQEPQVDFGQFNQIPQMNSQSQGPKFIFQQNNQIQGIQNFQQFQNNTSVPPIFQQKPFFIQPNQQQNTIQINQPQPIIQIPNKTQNLNHTLQSQQIFQGNIGQSGFIPQGQINIPLSQNSTLNKNSQGVQQQINPLIPPFQIPSDKNPNLTQPNQCKILVPPDKNQIISQTNNQSTIFSPPSNKNPIIGFPSLHNQQQTNTMFQNLESDAIKTLESMAQKCVESMITIGTFNFQINQNGNFVVYTSINPKLSGYFLENRGKNMLLQNEKNLNWETRFGVSIAQKENKGFIIKWKNNTYNSLSMNFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y028</accession>
    <name>A0A8S1Y028_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T1460088</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000146">
        <property type="protein sequence ID" value="CAD8207173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y028"/>
    <dbReference type="OrthoDB" id="5546453at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013233">
        <property type="entry name" value="PIG-X/PBN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040039">
        <property type="entry name" value="PIGX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28650">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS X PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28650:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS X PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08320">
        <property type="entry name" value="PIG-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035923158" evidence="11">
        <location>
            <begin position="18"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8207173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="352" mass="41473" checksum="7497FF8A8EC05761" modified="2022-10-12" version="1">MFILALFCFVSSQIIIGQLPSNINEQLTIKVDAKSIRISYCLSSDQYKNGLKKSEFNYPNPKTFTQLNHKLLLQYITSEILNEMEHKSALFYHENWRCKQYTIYDYFLEQFEISINNAKNRILTILKQEKQDSSEFKQSQDWTYQLQLNSNNQQQQIQINYKNQSNGPKYHYQRKLIGAGLHQFIETKITFDKQPSQNVVIVDELDTFTYVDKDELDKLPYEAKLMTIVDIERPNELSTQHIILSKHKGTQEITYIMPYHFRYHLASDTHYKTSYVPNIPRVYLESQQGDSLIKSFFNGNYQQAQISTYEQHLTYQIPVGYLPYKNVVVMITIGITLGLALHISNLIRREKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y3Z1</accession>
    <name>A0A8S1Y3Z1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trichohyalin-plectin-homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1490104</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000149">
        <property type="protein sequence ID" value="CAD8208037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y3Z1"/>
    <dbReference type="OrthoDB" id="309278at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="154"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8208037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="400" mass="49487" checksum="F57B7AF31071EDA5" modified="2022-10-12" version="1">MNKYSSNNPTGEWSRLIQQQNSQSDQQQQVLKYENYQRKLQYRQELQTAMQERNKKNSDSLDNFQYADQIQQSLQRQDFEQIQKLNQLKQKEAEFAKYSLEQQEIKKILQKQYNQNEKQQINEQMIKNQQNFKEELIEKQYKKNQIIYELNQSYKDMEDKKRIEQNKIKLFEKQSMQEEVNKLQQDEEQRRNFLNKLKHNYHQNEGLLEKYNQIYQQKEREKYEIDNKIIKEAAEQKRQQDEQRLIIERAIRQKKNKETYNSIMQQIEYKREWKEREQQQKSQELEYFGVVNEHYKRKDQDDLQRKLEKQKQYRQDLIMQIEEQQTKKKRDQSMSTVEMKINQNYYNNEALNVGIVPGIFVDHNRLKQQKYLDSNQTKRQNSQFQRQRNLSAYFPSNQIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y7Q9</accession>
    <name>A0A8S1Y7Q9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1570107</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000157">
        <property type="protein sequence ID" value="CAD8210095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y7Q9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8210095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="34" mass="4090" checksum="D6ED51E844E030D5" modified="2022-10-12" version="1">MKTILSYNHLKSLEEIDQFIQNSFQSEQWISLDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8A2</accession>
    <name>A0A8S1Y8A2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1530079</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000153">
        <property type="protein sequence ID" value="CAD8209127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y8A2"/>
    <dbReference type="OrthoDB" id="276422at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034245">
        <property type="term" value="C:mitochondrial DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006390">
        <property type="term" value="P:mitochondrial transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000041">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046950">
        <property type="entry name" value="DNA-dir_Rpol_C_phage-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002092">
        <property type="entry name" value="DNA-dir_Rpol_phage-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029262">
        <property type="entry name" value="RPOL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10102">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10102:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00940">
        <property type="entry name" value="RNA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14700">
        <property type="entry name" value="RPOL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01311">
        <property type="entry name" value="RPOL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00489">
        <property type="entry name" value="RNA_POL_PHAGE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase N-terminal" evidence="4">
        <location>
            <begin position="183"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8209127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1035" mass="119890" checksum="86FF3FAED85E7624" modified="2022-10-12" version="1">MIIIRALRNTFCRASISTSTTTSFPQVYRQKSQLLEVSESCKIINQLPHELIEQSCNIITNHFFPLQENYKRIIPKIEEVLKNPQLIKGTIFQGQDSQSIAEQIYEMVILIVDFADKLNIGFLTGDIHPLTFNIIQREIEITQDVDLLNENLRKSLFNIGKQYKLPLNGIKHPDNENPRNLFERQLIIEQVSNELALDKFQQVFDDLQRMSKAQHLFAAQKYIVEWFPKLCALVQQEQIQCFSDPNKEQQGYHQYITAIQSEKLAIIALSELMKEFIEAAVLFHEEKTTLNCYQIRVTRLANRIGEQIMKQHLYEEEQKNLRRKSKEDIQKMIEEGTIPQDQSEEEIQNQKELFYKQAVVKAGKLLKTQEEKLRERIRNNRIYLAKYGQAMPKDIASQIGMVLVHFLVDSIKFRNENNFWVPVLVQGYKKVKQDIEVAIFNVNQLFLHHLSAGMHKDNSYFMHLDRALPMIYPPAKWMDTQIGGYYLKPTNLIRIQDQSLQEDAAKRANLQNLYDILDNISKVAWRVNNKVLNIAMQVWEQGGGIAEVPKRQHDKTYVFEHQIKECKSYEEKYQLLKKIQSQRDLYSLQCDFTLKLGVALAFNGCSKIYFPHNMDFRGRLYPIPPHLNHMGPDIARGLLEFSEGKKLGKSGLRWLKIHLANKMGKDKLSMADREDYVDQNIDSIIKCAEDPIKYQDWAQLEDAWQSLAAMFDYVAAIKSPNPEEYVSHLHVHQDGSCNGLQHYAALGRDVEGATQVNLANTSKPGDVYTHVAGMVERRVEFDAKDPLSKDHIIALKLQNQIKRKIVKQTVMTSVYGVTFIGAREQIHRQLKDKGIIGDDDHLQYQASIYLARITLDAIKDLFEGAHKIKQWLIKCATIISNENQPVSWITPLGLPVIQPYRSLNKYDEVKTLISNVSIEADAANLPINKVKQRSAFPPNFIHSLDSTHLMYSSIECLKQGITFAAVHDSYWTHACHVDKLNELLRDQFVRLHSQPLLEDLRDSFQRRFPHLEFPPIPERGQFDLEKVKQSVYFFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8G1</accession>
    <name>A0A8S1Y8G1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1550030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000155">
        <property type="protein sequence ID" value="CAD8209538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y8G1"/>
    <dbReference type="OrthoDB" id="290858at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00195">
        <property type="entry name" value="UBCc_UEV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBC core" evidence="1">
        <location>
            <begin position="1"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8209538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="164" mass="18963" checksum="891C56135A3518B6" modified="2022-10-12" version="1">MMTAVIQKKRLQYEEENLIDNANQLPPNCSYKKMSDLAYVVQYIGEKGTPFEGAYIVVDIDLSDSNGSLGFPEQCPKITFRNNFQHLNVDSNGKLHTDIINKETFNNGTTLLEIFIQINYIIYHPSYVEPINKNLLEIKVLGFDFDDWMQGQSMKLRDLKYKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8G9</accession>
    <name>A0A8S1Y8G9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1560072</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000156">
        <property type="protein sequence ID" value="CAD8209850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y8G9"/>
    <dbReference type="OrthoDB" id="429991at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051291">
        <property type="entry name" value="CIMAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580:SF28">
        <property type="entry name" value="BOREALIN N-TERMINAL DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580">
        <property type="entry name" value="SHIPPO-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8209850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="255" mass="28603" checksum="C632663369BADCDC" modified="2022-10-12" version="1">MNTSFEQINKSPLNHSVSKQLYSFPKANRFKEFKDSACPNIYTLPTMMSKRGAGIGYGQKSDLIQESITPGPNNYQIKTTLNASNGWTMPVGRDKSNKYDGIFLGLIQKTPGPGQYEFQDSKSPIKYSMRMRTESQKEKDRKPGPGQYNLPEALNENGKYKISKFRDSGAIILSPPKAKSSRFSPAHELTPGPGQYQHTGDIDPKGLYYCSKFADTKSTVFTRAKRELTKIRVESPGPGAYKLPSEFGYYEKPIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8V4</accession>
    <name>A0A8S1Y8V4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1540061</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000154">
        <property type="protein sequence ID" value="CAD8209317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y8V4"/>
    <dbReference type="OrthoDB" id="282161at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8209317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="61" mass="7161" checksum="FDB82AFB704467B5" modified="2022-10-12" version="1">MYLPTFYKLGYEAWTAKSKRFMGILPAVGCWAVWALYPNLYNTLYTDFIPPPKGVQRRVQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YBP5</accession>
    <name>A0A8S1YBP5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1580064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000158">
        <property type="protein sequence ID" value="CAD8210217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YBP5"/>
    <dbReference type="OrthoDB" id="299557at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8210217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="179" mass="21211" checksum="4612FCE6D2CC5834" modified="2022-10-12" version="1">MQQRQQTHAITEEGRKSSSDIISPLREMYQIRRNVMKLEKQRTDESCHRKSQEFIKHISQSHRDVIIPQHLKFEDNLDGSRATINSINSVAQHYEIIKPNLHNIDWNKENNQNLSNKNKHHQICESNKHKTKFIQLDNQRQQINKTISQMSKCNRQLANVLKAQVDKLFDTIKTDLNYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YDC6</accession>
    <name>A0A8S1YDC6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1650041</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000165">
        <property type="protein sequence ID" value="CAD8211790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="297376at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="517"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1322"/>
            <end position="1343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1365"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1325"/>
            <end position="1335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1385"/>
            <end position="1394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8211790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1394" mass="165344" checksum="98CE842EC84762E4" modified="2022-10-12" version="1">MGACCAKKADREAGIQQQIEKTIIEQQKLREEEEEIQFNQGNKDYSTKRSIHVVQTTMYTNQERQDDAMVQIIQEEEKQPEKPVKKTKSKPTKDDINYFKKYEHVVHVKAIIVELIKNQVSHYYSLMMRVDYKDYIGEVKMNGILITSSEFHYYDIIPEKFSDANQYSRFSTFFSNDNYFNADKESINNFNAFEKLMPVERIVRIKQVEKRVRRKQTIFAEKESPSPVKNKVEFFDSRQQEMLEVITIVDIEEQERFQCLLFHILVQQQFPELNQYLVFIQNTTFLFDRPPQFDAPNIYRIALFYDKLDYSLKDLQVALTKDNKELSPHLHKKLTLNLLQILYNCYSKYIFRLNFTLSTVFYCAKSKGFKLESFGRLKSMLPFHHNQEMLLKSVEEKKITAYRKALKRDLFALCDLILYFKRIGQTTLRSIQAQRKKFIKMMDGGNDKDDQEQNDNYLHMAEERFSHNSDKYLFEFVKMVLFTTKVDSAETILQNLQSIIESLQKQISTYEYKLETQNDDDNKQQQNQNESQKKNEQVEVINLNEINLSQSDISNEENTNDNYLLSFEIDFQNLYHNLAKNKDKLSENESIYKDILYQSLFYQNEQYYYQLNNTLNFQSLSKLQQAQIIILNAFSTIKTMKDDSWEVKSQELSQCIEKAILIVNSINEDSYDNFHISILFQILSISQRKPLVILNQLQRCMTRQLELKQKLNNRLRGKNSAKGLIEQKKYVMVQTMYAQSYLNSNHYFEAITKIQKVVGFQLKNFHTASLLYGYSLMISGEIARLSSQPISAMLNLEMSLTIYNQYFPESIIQQTPQDEYIQQQKQKKEADQQCIQQEIPQYLKERKFENLSTSNLAKVEFLLGMSYFDSHDQDNALKSLRRAQKLMMRSYGIFADEVIALVLYQLNIYVLQDDVGAVMKIAMKYSSEMNKQYKLGKQFRRKCIAKLQFIIGCIFQFCGSYLSALRFIERSNRTLSNAKLNNFIIQSHYQAKKMNIIEQVKRAFHKIKSKQQLRGQLDLNQFQSLNEVLGSSFQNQFFCQPTKYHASILFKKYSKYILQVQGLIRIGENDKAIHQIEELLKQASKMKKDELYAYMKDKLVLITIESTQTKDQILNVQRLIEIHDRYLQFPFKQIYKLRSYIILISLYAMMKDLKKFNETQSQIDDFILDIYQIFNWQVAAPIDNTVNQGKFERLKNRNKVKILFQIQQIMDLIQYFYTIRKKLFDAFDNQQQLKDIGIQVLKRLTGQTKPIFQKYFDKKQECLYGIQSKVKIKNIEKEIDIQKLNENQQQTKIEVHLQPEQVDDQQNHKLVFDLRSYEEIVQSRNNKRKSSRHQKQQSQEVQIEKKEISEIKEIKESKHKRNVTEITAATLNKNKKKQAYKLNPFETTTKNSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YDF6</accession>
    <name>A0A8S1YDF6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1650056</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000165">
        <property type="protein sequence ID" value="CAD8211820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YDF6"/>
    <dbReference type="OrthoDB" id="291040at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8211820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="263" mass="30924" checksum="3A88944C9D364C92" modified="2022-10-12" version="1">MEPQFEINQSIQFQLCQTQSLWENGVIITIFTNKTPIQYVVQTSNNEKLYLNEQQIKAIKSVTKTHQVTYNIEFQRILSQLIMQKNEDQLIEELKSLLKRITPQQVMQEQNGAKIIYTTYQFLIMRKPSILRNYPFAPTNLVLKQLKQLVYAIKKEVLKNSNLIQLQTHSNINQNSFKHIKLIKQTQKLSRKMTQIYDDSEKEEQPTAFYANLLPNKSAFRPYKSDSMNHQLEQLSIQTKPSPKIDQIKTIYFKPIIIQGLLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YDU8</accession>
    <name>A0A8S1YDU8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1610112</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000161">
        <property type="protein sequence ID" value="CAD8211017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YDU8"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004771">
        <property type="term" value="F:sterol ester esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002168">
        <property type="entry name" value="Lipase_GDXG_HIS_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23025:SF3">
        <property type="entry name" value="HORMONE-SENSITIVE LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23025">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01173">
        <property type="entry name" value="LIPASE_GDXG_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="3">
        <location>
            <begin position="415"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8211017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="654" mass="75479" checksum="5E149FA685B8A976" modified="2022-10-12" version="1">MQFPKTHLNDFISSDYDRASEIDDYTLFNNVCYKSSQAQVPTEIQIQIRNTDLCQSQEQSLQATRDIIDKLNYIIKILKTIKCESEEKQRIFNNANRHFFILLKGLQIVDELLSNCKGKESKILLELKQNYVQERQIKALKADDVNIRMLQVPYHYLQNKFLPKMLQHLKLHEQHMFASQSLTDEIKVFGTEILMLNLIMPSAIDTDKNDIMSLNSTHPVWKKLECITTRKSLGDPEQIRQSFDKFVQSVMVGHALVNDCKKTTSKWGKIFSVSLNSISAAEKGMKDHMSQLRADTAYQAMNFIELPGVKHLINLGLPKISTNLKFYILPVVLPLTIQEANNQIRQGIYDQISNQPIEYVNQEIHRDDKFLFVKNKARIRIRLLYNQHVVEQDNIFSFLKSFFVKQNNMIEDKIIIHIHGGGFISQSSSSHQCYTREWAINLNVPIFSIDYSLAPQYPYPIPLDDCWQAYNWILNYSEKIFGITPNKIIVTGDSAGGNLATALVGLTIKYKLQIPNGLILNYPAVDIRFQYTPSYLKSINDKILSHTILGICIESYSAHPDSQLGIDPFMSPLLLSYDILKYFPPTRLFCGDQDPLFDQVFRLAQRLQVVQRDVKITIYENLSHGYLNYNTIKGMSEIKQCILDNQDAFFELLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YH27</accession>
    <name>A0A8S1YH27_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1700061</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000170">
        <property type="protein sequence ID" value="CAD8212708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YH27"/>
    <dbReference type="OrthoDB" id="292834at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8212708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="62" mass="7548" checksum="FED70661314F3217" modified="2022-10-12" version="1">MNTRLPSNNMYNIQRPFKRNINQNLQDAKDYYYGLLSGDNEFQKECKKVEYHNPKDLSWTQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YL63</accession>
    <name>A0A8S1YL63_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T2250003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000225">
        <property type="protein sequence ID" value="CAD8214560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YL63"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8214560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="23187" checksum="5C55424D7A3863BA" modified="2022-10-12" version="1">MTTALDFNDTSFNKQKMSTTNFQIYKFYERVRYRELLCKKIKRFKKFQLQCQEKIGIKFYFICSQFISCITFYFLFSAFIAIQLMHLEIHIDFRFINQKYKWQITQDTYFSSFQENKKQTILIQCQATNCLFYKKLVSSYRIYKLISQHEMLEFLHFKKYNLGYYMKIIQFISEPRFILSQESFLILF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YMY1</accession>
    <name>A0A8S1YMY1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1870020</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000187">
        <property type="protein sequence ID" value="CAD8213827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YMY1"/>
    <dbReference type="OrthoDB" id="409374at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8213827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="417" mass="48635" checksum="F101BC6A570A2E83" modified="2022-10-12" version="1">MNEKRLKQYSKKKCGDLIITCDEECDDGNQVEYDGCYMCKYSCPLNCSKCQFGKCKQCQPRYELINGYCKYNCDGYEIQDDQENRNCINRINNLIENGHYQHNLFNNENSKYTLVSSLTCNLQDFGIFGYFYNQCRIPVIQYCKESLYDKCLECDEYYKLEYNKYKCTPLCNDGIVIEREQCDDQNNIQFDGCYKCQQSCLLECINCIENKCYQCLDGWQLIDYSCYQYCGDGQVAKSSMEQCDDGNYNDGDGCYECKFECFPFCRSCADRNTCLVCEQYFELYKNSCKPICGDDYIIIGLEECEDGNDIPYDGCFNCQFQCEKECQICQLGLCIECIEGYIILKDYCEVYNQTQIMDDEEDAQIQNKCGNGIYSNDEECDDGNLVDDDGCSILCEIESNWFYLICLIIIFQQSQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RXC7</accession>
    <name>A0A8S1RXC7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0010433</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RXC7"/>
    <dbReference type="OrthoDB" id="288452at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23244">
        <property type="entry name" value="KELCH REPEAT DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="335"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8132082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="496" mass="57492" checksum="C656A8FFE9751179" modified="2022-10-12" version="1">MSWITINSQNEGPGGRWGHSSIALDDKLYIFGGFDGNYLNDFYSYDFDNNLWQTIVQNGLVPEPRSNHIMFINNGTIYIQGGGGLSKTRFGDLYSFNKNTWIKQKINLIPRTYHTGCVGDNKLFIFGGESGRDLDDLEIVDLINNTQYTVKVECTKKPEPRRFATLQFYNNQLILIGGCTQSYSLTPSIYIANFKENDNYISIFWSLLKTEFPKWGQSCVQFDDLFYLFGGRDDKDSDELYSFDLQNNQIQIIKNENKIKARRRHSASVIGNSLVIFGGFNGKYQNDLCFQELPLVNSIPLSVELLSKPYEEISQHQFENYFQSENGIYFYNDQYDCLIQTDEKCFGVDQQVLINASGYFRELFYGDYAEGQLLQLDLSYVDNIAFGIVLIFSYKNNLILPILNKEELFSLLELCDYLDMPQLKEKTQYYIAQTVDKNTLQEIYNLSIEHQNNQLQHFCAFKVSKLKMPLQEIDNLQSQQVNNMKKIRKLSLVNEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S1X5</accession>
    <name>A0A8S1S1X5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0020129</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8132904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S1X5"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14003">
        <property type="entry name" value="STKc_AMPK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF150">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE STK11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="12"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="9">
        <location>
            <begin position="268"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8132904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="547" mass="64513" checksum="8E8B30BFA79D754E" modified="2022-10-12" version="1">MEDEEYKQVQNYILQRTLGQGTFGKVKLGYHIIADEYVAIKILEKSRIEKQCDLTRIQREISILRKVCHPNVIKLYEILESESCVYLVMEYVKGGELLPENIAVRYFQQLVFATEYLHSQNITHRDLKPENLLIDENRQLKIADFGLSFISETKGEYLKTACGSPCYAAPEMLLGKIYEGTKSDIWSCGIILFAMLCGYLPFEHENTQQLYELIKNSDFEKPEHLSKNAIDILTNILVKDPNKRFNFEQIKQHPFFQLQTSIPTKNLSLNDQTIINKMIEMGYQQKQIIHQLQSNKHNIITTIYYLLQKKYNQPHRRSFFQNIQNIGNLKLDINQKRFTISKLQSSQQGSPILEKIDKIKQSIIQKLENSPYLKTQNSQKFKKFNLSVQSKRVSIQIENQRQKTNSVQEKHYDYPYTYTPNIQDQKSRIQSRKNSKNQTPFKLIQRRDLEQVIKTDINEEILSNQKGIYAQVRQRNKTQPNKNEEGQNYFEKTSKLNTSHQMVEKTQQQSPYINMNFINHRQLFKQIYNGNLSDFMKKKSEFKARIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S5A0</accession>
    <name>A0A8S1S5A0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0030161</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="293715at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043601">
        <property type="entry name" value="Rspo_Fu-CRD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332:SF175">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15913">
        <property type="entry name" value="Furin-like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035828276" description="EGF-like domain-containing protein" evidence="6">
        <location>
            <begin position="17"/>
            <end position="3950"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="3698"/>
            <end position="3715"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="3807"/>
            <end position="3826"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="3832"/>
            <end position="3851"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="3863"/>
            <end position="3890"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="3902"/>
            <end position="3924"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="3377"/>
            <end position="3388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS01186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8134154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="3950" mass="454183" checksum="C7C58B3CB38D9827" modified="2022-10-12" version="1">MFHLVFFLVVCSYVYAQTKLQLQGPLPLNIDCKVSSLIENGFIGNQYFYNYLSQNGALLSLNKITFSLWMQVHSDPQQKDRQIIFCFFDGNLQSPNINLMLYYKPEADGTYLYLENQKVNTIIIKLKIAIVNKLWNFLFVSIDQSSENCFINLKFFLTGDTSTNPQFFQYAETLINQKLIYKFNDRSRITNEKLVGFSSKDQKACLNIANFYYINGWTSMDKEIYVDYDAELKFELKPYLQNEIIVSNTFPDILLRKNTIPIEFSGNIGMNFYKNTQIVYNFFEQLPSLTIMFWVKPQNILSSFQFLSFTDDVLQETSIGFGVSKLYKLLFYQVYTKTPYCDLPQNKWNHVTIGILDISFNDDFKQLNQRKLMRIFIDDVERNLTIITNIKPYKRLVLGPVFMDDQGLEIIDIQDIKIFKGFGIRQTFNHDCLLFVGDYCAFCKSGTHYCKEQDPNDDTKIYHCPAGYKEENYKCSKILIPNCLRFSTSTSSCNICEQNYELINGQCQLKTLIVSSPFKCDDSNAQLCSFNSALNVILKDLDNKQISKLCSNNLYSSTQLKCAGLAVPSCTKSQFRSQCAQCNNNNTLTEIKDCKPVCLTNLRFSGPNNICVSKCDRYFFKVTCIGRNPRSEYYCNPSKNCSSTELDLGYYCLPKDQSVVGFTTFCGKTYQKCHSSCKYCYGNLMTNCLGCFKDVYYNPYMTSCIPDCSTTKNNKFYYSNKYNWTCELECPPWLYTENLNCVKECSVGFKLFQNQCFKTKLIDTYETVKEIPDEVAPKQIDILQYCPQFCKSCTSDTLCTSCLNQYPMDQDMCVQSCYPQYLEIIDNVGSCRASCNPQDLIYENNNINGYQINQCFKEKCGTIRANKDYQTFLHQTDTHRCMYPCEDGYYGNTLLLQCLPCSHTCATCEQSAIFCTSCPYFKFLDSNNCYDSCQGKYENYLNSKCEGSCSSGYQVIEQSQNIYACVESCGSNYPTYLYSLNNRCYEVQPVVGAYCEGFNCYNCNYKCKTCSGPNSNQCLSCKPKSYLKNNECVKQCYPQFSDSLNWLCVDACPTGSFTTYGPEIVNGNSVIVTYCSVTCLFNQYQFKELCYNSKPLETYCLLRTNYYFCDSCASVCKECQNAYSTSCTKCSPNAFIYGTTCYTDCPSIAPYKDLITLKCVSSCTYFAENGYCVYQCSDSYYQYPALKTCYQMGCPMGTYNLQYTKLCFSCYTGCATCKNGNSSGCLTCSDGYFMNGGTTCINSCIIEPDLVQDWVNGRCVQQCPQGTYLRTLDNGALACTQDCPKYYYSNICVSACPSQTYLDKQICKSCAGPCSECFGVKVNECTKCDLGYYRDETTCYSVCPDLKPYANLAEQTCVAVCPDYLYLKKNYCYSPCPTFLYYYELNDKKECVDQCYSKSYLWLGECYECNSICKECFGPNNGNCLECELPYFLNEQTCQDTCPQFYDLIDHVCKDACPVHLVIQGVNCQNECDSGYLVYNQVCVQLCPTFAYLVDDHCYDCNLYCSTCFGPTADECYSCIDNYFLDESSCKSVCPLYYNNETNKCMADCSNMFEITEKKECVSSCPSNYIICESKCVLVLQDGYYLDGNQCQQCDPKCSQCTSATVCQACAKNFYLEVKSCVSFCSNEYLFMDPISRSCVTKCPSTLYHLESFGKRFCVADCNYKLYSQCVSECPKGMYSKDSVCTKCPQYCEECESATICSKCLFGYYLQNGQCQLSCTIGKTDRKNIKCVDECDASLFEYQNECLENCPTDPILYNNKQICAHSCPQKTYQNQQECKDCDLSCALCIGPSDKDCIICSDGYYLDNQICSQTCPQLYDEVNKQCVLTCPTNLYQDNNKCVESCGTYQYINNCVSSCPALTYVQNSKCYDCSENCNECNSLGCIKCALGTYLNDGLCNNYCPYYYNYVNNECAQLCPKDTYIYIDHCYSQCPLSTFQYKNDCLLACPQLTVLLNNQCLQCPERCLICKSEYECSKCESPYFLFNGSCVIHCPLQLPFEDITNGKCLSACPPEAYERGYECVKECNLVIYQKQCLQKCPTGYYGNDYCKPCRLECKTCSSFEVCTECNDNFFLEFNQCDTLCTKIKDIKSKSCVDTCNLFLYKNVCYEACPQNTYENINECVLNCNDGYYGSQDYKCKKCSQECASCINALECTKCNIGYFLHNKKCLDQCPGQFFSNPVNNECSTKCPIQTFTFQNSCLFECPSEYFNDMDNYKCVSNCGSQQYLSKKNCLKCAIECNCTNRSTQDCSCQKGYYDDEFYDDCQKCPCDECTAIDNCLICKNNLQVPKCSCDRILNEDWCITCQIAKVNIYFTDDLNQIIVYFGYLISVNLINPFQPSDCSLWFDNSEIFGKNSQCYLSWNRYAVHIILKPYASVNIGDKLSFKNSFYRDIDQGICDGQFIEQFIENKVKEPQNLQKPYILFDIPSFVSTCKIIFIKQILLHGTGQKIQNVLSWTLKEMEDEEQYLQMDAFLANQQNEITIPIGTLQPNVTYTXMVIMVLKTINAKNVHKNVLVAQMLQNVLNVILDIFFIIKNVWINVQDNFFLTLQIMNAQQNVQYKHLHFRTHVFLNVLLNILTIWIIINVQAIVDLNNIQVKRTVQNVQQNVINALEEETIIVSNAQAVFNQLKMAFAQATSDNSCQKCLHKCQTCENGTDCIKCRGTNRSTQDCSCQKGYYDDEFYDDCQKCPCDECTAIDNCLICKNNLQVPKCSCDRILNEDWCITCQIAKVNIYFTDDLNQIIVYFGYLISVNLINPFQPSDCSLWFDNSEIFGKNSQCYLSWNRYAVHIILKPYASVNIGDKLSFKNSFYRDIDQGICDGQFIEQFIENKVKEPQNLQKPYILFDIPSFVSTCKIIFIKQILLHGTGQKIQNVLSWTLKEMEDEEQYLQMDAFLANQQNEITIPIGTLQPNVTYTITAKYVNFLQRVNFTTFTFTTLPIQAPYVFLSYEPLIARVYVFDCHVSYSDLKNEFNVAIEITGSNNESYISLSQQINPIYEIPLNESLLPKETPLLFTASTGSSIIKETINLKSKIIDIKFLQKNRFIGLDNQINARGFDRNIEDEVLSTIGITYEWQCNNLNNLQPCKTDENQIMQFPSRRIVNIFADSQNTTLVFFAKASKGDRWTVKEQLIVITDFNIEEEFYINNEDPQNYINANDEVTILLKKQQKYAFIIQQHKILQSMKISGLSLKFRLAGLTTDFKSPVYIYLVPGNESIAFKLNESPQKVFLQIEPLIGESLDYFNYTLSNLQAGNSVSFYYYFENSILENDVNAQSIINGIPLITNSKETSGSFQLPNGITNNSISVLCEISSNYGAKSYLKQDVYVVRKNYEMQQLYQSFTNKTNLEDLQSLHTMIALIQLEEQQVCLKQCSGVGSCVNKKCQCPPGYYFPDCSGNQEQQIQFNKFMTDVMQQLIKTPIKNNNDMKLFSQSLYFLTQFSSNNTISLEDCSIILQSYIQNTNSRLEQINQYSINLQYQSTDDFNYSQIDIRSLINKQDLHTALKSTVFMWEKTLFTSGSNVYQLQNHLNNFINNIIELTIFALSPDEKIDFSIDTASITIKKSKTLPYNSSRILQEINNENSTIYDIVQAIYIRNFYAFDGYFPYPQQIYPLYVYQIRYSNRKENIQLIQPISYRFGIQNDTTNLVCLQRNAKTYEWFNQNCTLSEINSTYYCNCTTLSPTTICNDYDFIYQGNSGFRINLPNIFYIIYIIQLIILGIFVSKSKMQENEQQTENTKFGRVFKLAKNKKVNIIGNQVYPVEQEKDTRELITKPQLNNQDNINDKFHFNYFWKYHCLTQLIFKQQVYLSPFMRSLLILLRWNSAVIVAEVLFLFQFDYQVSLWIILASIIIMRISESFLKKQIKYVVIMKQIIVVIIIKSLILLLLVFTILCGLYFYLMIDNQTNLILSYFGAILIDILLLDIIIFSSKRFLGENKKKNIRKKIKELKQLAKIQKQND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S6E8</accession>
    <name>A0A8S1S6E8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0040462</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S6E8"/>
    <dbReference type="OrthoDB" id="10494777at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="103"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8135866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1118" mass="131260" checksum="3F9500BDAD7EBA99" modified="2022-10-12" version="1">MNCRFHPQTPISLICTAPHRCEINRKMCVECQQDHGIEVKQTIPINKFRQMLNGKVKDYKLDDTQELNKQRLDFKYMLSKAVEMLKQLWEELSESIRKIFEMIEKENKSLTDLITQNENLTESSYSDLEKLVQLLNGKIVDNLNNKKNSIMTKVKKAKDLLEKEVIIFCQKFKEEMREILSIFQVQNDEQIILQEGSQKLKVAICDHGRFIYSNLTIQSNQNKEIQYLQDGSIIRTDQIRYTAGKPEILTNLEQIKHLQWFGQLRENNHKVGIWTATWQGEKIKDVGGQYSNEGKKQGKWQELIENYSMQSNAFEIGEYDNGTRIGNWKYLYDNKEMYKSQIQYGIRNGGNYNQQGNKNGKWQEIWEGFWKDSQVFYNGEYKNGKKVARWDIVFENKKIHLFACLVVVDHMMIEEMGLNRVIGLKFRMDFLEILNWCIVVNIKMARKLLDGIFVKLKKGFYLVLNIRRCKHLIYKPQQKNSSGGGSYDEGDNGIKQGNWVELSDALFRDSQVTYSGQYKNGKKVTKWDIWYQENGHNQKNLKIGGGSYDEGGDETKQGEWVEISDGFYQESQVTYNGQYKNGKKVDRWNICLIEKVYQMIYNPKKQEVENMMHDNFSDSNQITYHGEYKNGAKVGIWEILKMNKTDYFELKLENQFHIFIQDSNIILRGKIFNGKRAGKWDILYGKIMIGGGSFGSQDEKSHGIKCGKWVEISEEFNCNSQVTYSGEYINGQKTGRWNIYQFKENQFIWCGSQIYDFQGNLIQKENNNASIIYMGNLQNGRKVGRWNILFQKNYEDKQIEKMQRYQIQQCVVFSGGGQYEEGVDEVKQGNWVEISDGYTDRSQITYIGEYKNGKKIGNWDIKYNRFENGWKNEKIGGGQYDKEDNGMKSGEWVENIENFTQDSQVTYQGNYKNGKKIGRWDVWYKKNGDNRENLKMYQKYFQSIIFVSLFSGGGQYDTEGNGIKSGEWVEQIENFKWDSQVTYLGNYKNGKKIGRWNILFRKQFDDEYKLIGGGQYDTEGNGIKSGEWVEQIENFKWDSQVTYQGNYKNGKKIGRWNILFRKQNEDQSKQIGGGSYDEIGDEFKQGNWVEISEENIIRVKKLEIGWRWIYIIIRKVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SEA6</accession>
    <name>A0A8S1SEA6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0060450</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8138100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SEA6"/>
    <dbReference type="OrthoDB" id="303788at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8138100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="329" mass="38936" checksum="F2578C726AF97C55" modified="2022-10-12" version="1">MRNTRCLEYLIDQFHLDDVIPCKDQNALICSNENATIIVIYQQNQLKHYEEIEGRIKLIFQQELCQFKRQYYLQQLELPTKIALNKRGDILLIEINKTKFNLYYKKAQLQNWTFFYHNYTQTVNLQIQDYKIYGSYTLKSFHSQFSCFLLQKKENTTTIVLNIKNTPQIKKLYQQQIEITDCILNEVNNLIVGREFNQLNIYKWKKIIPFQQINLPNGLLYTKFDSFNNLILLTKAHLEIYVQQIGQTYQKVAGIPIINNNQISDIVPIDAYIIIQRTYLGAEVAQVMLIEDNNVQNVGKITCRSIITSPNHSFYLVIKDNQIEIYRHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJM7</accession>
    <name>A0A8S1SJM7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0080114</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJM7"/>
    <dbReference type="OrthoDB" id="286239at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8139469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="426" mass="50619" checksum="39CDEED8DA931CD7" modified="2022-10-12" version="1">MNANSFLTTKCTSFSFTQYKIRSHSIQHKSFDNNHSHPSQKDLLEKQKQQRRSVHVTSQQQQNVNINQLFTKPIIEELMWRQSELYMNQFKIQLSYIVDNGNLQQFGAIICMTADILLKIKDYNNACYYYNQYRILNTLTKNHAEKAKALIGLANCAQEIKMNSEALTLLKKALQYAWLSKEYELQIYEKLALNYYYLGQSEEQYYFHERGLLGIVESDESPIKQFSCESLKDQLKKNNIEVKTLCPQLLNYMNLPILSRNELLSRNPNSIRGIQVKKQVIYTVTEQIEIILQKNEEFQYQINTPKYQKLIKYQNNKKGDDFRVVNSSQSTYSKERRQYDLNNTTIYKLPYEVQVSNRIRNPYIPEDINAKLHEQLRNANRTQKFGLRNKVILNHQNREDMHYRKNGACLYRQLYAIFSYYSHLFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJT8</accession>
    <name>A0A8S1SJT8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="5">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0080154</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="5">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJT8"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07852">
        <property type="entry name" value="STKc_MAPK15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000238">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001058">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="13"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8139549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="494" mass="56801" checksum="1376BA6672989E6A" modified="2022-10-12" version="1">MSEKIDSNILKKYEIMTKIGKGAYGIVWKARDIKSKRIVALKKVFDAFSNPTDAQRTYREVSFLKQLNHPNIIQLIDTYPAENQNDLYLIFEYMETDLHIAIRAKILQPLHRRYIIYQLFKALKYIHSSGMIHRDLKPANILLDSECKIKLADFGLARMVCTLEQDILTDYVATRWFRAPEILLGSKSYSFGVDMWSIGCMIGEMILGKALFSGTSTINQLEKIIEVLGIPTPEEIQSLGGQNQLFDKYPRNYKSNLKTILNSDEDELDLIKKLLVYDPNKRISVQDALKHPYLKEFYNPKEEITFKGQLILKLQDDKQYPISSYRDLLYKQNLDLTKILTLVNKQKSLIQNIQSNRSDALKKQSSQQQFLNQQFAKSAFMKSNSTVNIEKSVNKTATATLHNSNSKQPQNSNKSSSTQIKNKLKQICLQSADKCEVVVQKKSPNSKISQQPKLLIQSNSKQQILNNVTNTSISPSQSQQRLQIVRQQHQQLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SKH6</accession>
    <name>A0A8S1SKH6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0080320</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="309210at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1088"/>
            <end position="1124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8139839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1317" mass="158016" checksum="5719D167F4F5D916" modified="2022-10-12" version="1">MNFTINSNIFKIQNHEQMNQFINFLKLNGQFTNGEFHFGDQQHEKKFTIFQQAFQTLKENFPEFKLKFKCHKGNQQGSIDIDQKMKSEDTRQTFQTRTNDQSISLDQYRTFENQQPQMQVQQEVKNIESHIEYKKDESLKLQQSEGSDEESEFDNFQDNNLKQTISFEQNDVFQLEDNEYITELHSDILRQLHNSECRNLQFNSEVANQDVVDSEIKILGLDTAQIIKLQNDLILKIVQKFFLQEWNQLINSLNLEQKKMKMNLYRLLIIQDIEQVKKGILDFINKISYIDIEFCYYTNYTMIAESKINEFLRRYEIYPFQHFDNFYLVSKTQNVSQLQEISQKINNNYRGIFLHFSSLRPYKQEIQYNKDLALCRSLYYEKNLVEQFKQICMNQYFDEQYFFLEMNFSHVKQDRDLFEKQIKKYIQNNLKCPLLLKFTTQCNQKDEKIIDKLFQDEIEAEQLNIIYLNKEGTFLIFGNPEHLLDIFKTISAKQETQKTFITQTTLFVDRQNASWPTFSQYKPFDLDALPQIMKELGFKKQQSQESEMIFQNTEKINFNHFLKIMEMICVIRQQNQNKEKIKSINNKFLLNPYKAQLNRLDNELFSLSQEGCSKGKKETFSVTIDIVQNNIKQFSCSKIIELKPKFLDSIINDYFYQDFQEKLVQRNQQIQFIKIEEGKYQLSSQNSQVIKQFEEYFSKFVTQKLILNQELLSVFQFSNYMKQEFSKKGVYYKLEPDGIIFISKSMDSINNVIQSLQNYRGFIITMHSLKSQNLEEKLENYQLVQARIGYMKYILGDFLQNINHHQQNVKIMEIEFKEEQEISAFSIFHLNFDKQRINFNSQMMDSATILLQEDIISMIQHLYFFELKTDQETGKIIFLNIIKNPKIYVTFKQQYSDYFITMLGNQKQYKELYENLIQLQNNQKQQQNIEFSIYIDQFCVFQNIIQIQQSKKKFKQDFEKKGFEVDDQKIKWIIKSKKPTQSFQILSILVNKMIEEDESRYYQDPNDKNDNFLSNISAIKQEGSSFQSKSFIYYYQDFFEDWQSKNLSNSIYQDETMKKLQQITINKNEIYLPQKQQLSQQTILSFQNEDFNKQNIQVQQQQQQQQQQQQQQQQQQQKQQSTNQIKIDNILQNQNQYDNDDSAQMSSSQISNSQSSQIIKESYQFQSEISPTFEKKCLLVEFQKNLISIVPPLDEKLVQHLTQNLKNEKFEYFEPQVNQDYINYFHKIAQQTITLYIIQKQDKEYHNEFSKKLLYYLNYEVLIEKFMSHSSLIIICCKANKAARYQIPNKDKSYQICLKDSNQVLPFLWIRRKTIQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SML7</accession>
    <name>A0A8S1SML7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0090240</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SML7"/>
    <dbReference type="OrthoDB" id="285444at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8140706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="72" mass="8371" checksum="AA5604CC68BE5AA2" modified="2022-10-12" version="1">MDKKLLSTPSEKLAADAADEMTCQNHMNTYTTCLQKHSLTNNYKECVKYMDQFRFCLLMAAKVQMESQKKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SPA4</accession>
    <name>A0A8S1SPA4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0110092</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SPA4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8142343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="613" mass="70606" checksum="E0409934C3460F0C" modified="2022-10-12" version="1">MYSCISIKNELCFWHQKTQTCKAIINLKNKIAKQEELIQTTCQIIGRTPTSCSLLNFQMPCGGSQVGCDYVNLETAQCNQVGLNKYACLNLTSQSCKWVLNQKLNIYQCQEYTPFGLCSEQPQQVNALVCSLVGHNDPCTYNKFSNSCQWPLEQEESCDMIGLNQYGCAQIENCVFFNGKCIKFNEDLNLNCKDADKAHYKVCAQIKRDQCKYSELKKGCISTDLFDGCQAKGINQLGCNAKDPMCSWVENNCECVKLLKEKIPCYQIQNHYDCQQRNDCYYVNSYKSNIDTDVIKLGNQGRCKEKQCSDRSKSECEGQIVYGHICYLDKQGICQSAHDCKDIKNAVQQCSNYLIKGSPCMENINNVGECEILKNCQQLDMINCQRNLDYCIYNSDKCMNKQCINYMDENNCPKLNCYWNYIKKRCLEQISCELNESEKVCNESHNGNQKCGWFKLDGYQHVCTSGCRYLYQAHVNCQGTQIRDSVCINYKDVCIQCEEITDSCLCLEQQEYCTYDINRNICQSNGCQNYNQDTCPTSRCYFNLNKNICIQQCRFRYNNQECELLNDCYWDYIENQCLEYYKSPQPTVVNPSIIPIEELLIKALLVISLLVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SQH0</accession>
    <name>A0A8S1SQH0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phospholipase</fullName>
            <ecNumber evidence="6">3.1.4.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0090228</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14445"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="3.1.4.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the phospholipase D family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.4" evidence="6"/>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SQH0"/>
    <dbReference type="OrthoDB" id="419078at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004630">
        <property type="term" value="F:phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09138">
        <property type="entry name" value="PLDc_vPLD1_2_yPLD_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09141">
        <property type="entry name" value="PLDc_vPLD1_2_yPLD_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016555">
        <property type="entry name" value="PLipase_D_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015679">
        <property type="entry name" value="PLipase_D_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896:SF76">
        <property type="entry name" value="PHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00614">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009376">
        <property type="entry name" value="Phospholipase_D_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="7">
        <location>
            <begin position="363"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="7">
        <location>
            <begin position="794"/>
            <end position="821"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR009376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8140682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="958" mass="113016" checksum="B79981E4DA8D6F72" modified="2022-10-12" version="1">MSYLRRNKIVQQDSNLDQQQKQQFQVEGLNYQVFHQYIENKFTDSKQFKFLNGNALNQNVYEFQISYQNQKWRKTSHMFNLIDNIVVQDNFLYLINYLKEVLEHSEFNQKLKQFLQFSDVQVNKKYLSCYVDKRSGGRHKRANCRERLTCCHQQFQRRYLTLTCEGICLSYEQFGEQSRIRDMILFDQSFQIHYGKKQTDQKYGIIFKNNTRNLLVRCYNEFEFVDLVVQTQIVFNQSQSVKKNPFDSFSPIRNRNFAKYFIDGHNYFDQLRQDIEAAKEEIFITDWWLSPELYLKRPSHENEKFRLDKLLQQKAIEGVKIYSIVYNEPKLALTINSQYTQTKLNNLHSNISVVRHPNSVIPMLWSHHEKIVVIDQQIAYIGGLDLCYGRYDTQSHPLFEEYKGQFPGCDYSNSRISDFRDVTNFKQSDINREETPRMPWHDVQIKIVGGSVRDVAKHFVQYWNFVLIDLTKKNEYSILQLQDNLESLNRWDRYKLKLSQISSRLEHFLLTKLHLSNENNQYQQQQTPESSLASPNINNQNVQIQLVQSQIQIQSNQSEEKQQYIKEDKQRTKSASFYMVKEGFQIEEDILNAMNKQSQIEDSNYLFQAKKRTPSFQQADKPQINNEKIKLDVRKTSKSGSCSIQILRSANKWSLGLSKNHTENSIQKAMIHLIQHSSYYIYIENQFFMTSLAGEPLLNPVGLAIVQRIKQAYYNQQQFHIMIVLPLLPGFEGEIDDNKANLMKLQLHYEYYSICRGGQSLIEQLKDIPNISQYLTISGLRNHGVNSKGQPKTEIVYVHTKLMIVDDSKVLCGSANINDRSLKGSRDSEIAILIEDSIKIGEGRRFAYDLRRDLYNEHFHITDYRKPNDPDLVKQIQFQIRNNTILYRQIFACYPDDCVLTFKQLKQFKSSANLQSYFTLQHEIVGFGVEWPLEFLKEESLKLPVFAKEVLVPDINFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SSJ5</accession>
    <name>A0A8S1SSJ5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0120428</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SSJ5"/>
    <dbReference type="OrthoDB" id="444540at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051581">
        <property type="entry name" value="Ca-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34524">
        <property type="entry name" value="CALCYPHOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34524:SF6">
        <property type="entry name" value="CALCYPHOSINE LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="30"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="66"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="211"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8143835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="278" mass="32770" checksum="99F03473AB026650" modified="2022-10-12" version="1">MAYYGVGLPTAIQELVFRFRNHICKANPYCTLSRLKAIFQQYDKNGNGKLNLEELDNLLKSVGMFLKIIELQALIKYFDKDRDGFLSFQEFLTFIREEMNERRKAMVLKVYDSQQNTKITSDYLRQIYKINNLTDGMKTFINNICVGDELTYEQFLEFHEELSLNIASDETFTSILSKAWNIDEKQEDTAFQEQLQNSLKQIKERVIERAGGKIYVMNVFKEFDTNKSKDLSISEFIFLLKKLELDWNKAAINRVYRIMDQNDSGSIEEQEFINFFNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SSW7</accession>
    <name>A0A8S1SSW7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0100198</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SSW7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8141572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="244" mass="28660" checksum="34BB0C55373D59AF" modified="2022-10-12" version="1">MTKICILTKGQLLGEEIVINEDGCCEYNSIVLSNEATVMVIHKHEFFQKFPEECKQWVKEEYYKKSQFRKIFQSKSMESVFGIGKPKFITVKQFIDRKNYPIKINSKQYEDRVEIGSLIEKKTSVYLQKYQIEPQKDSTATLFGYNLYQTPKLGYIPQYDKCNNIPLKAFKEQYLKKLCYKKRRLEFLRPPPLTARILLNLQTNQCEQLNKIQHTHTHTQSTAVNSFTPCLSSSPNYVNKLDIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1STD0</accession>
    <name>A0A8S1STD0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0110271</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1STD0"/>
    <dbReference type="OrthoDB" id="27537at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001784">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002022">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037867">
        <property type="entry name" value="Swd2/WDR82"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19861:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19861">
        <property type="entry name" value="WD40 REPEAT PROTEIN SWD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="108"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="260"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8142656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="341" mass="39114" checksum="95F0EE27A6256DF7" modified="2022-10-12" version="1">MHEEQEVEITTDQLQSMKMCKIFKDHQKDINAIDFSKDGQYLVSCDDQALNVYDVHQGKKMRTLYNKVKEIDLVRFTHHNSCVLCVTKKEPYDIYYWSLHDNQILKKFTGHTNIIHWLDLSPVSDDFLSCSMDGTLRLWNLASEYQTSDGLLDLSAKKTFSVAAFDPSGQVFAVVFLEQYYCISNNWLYLYDFKKYHSGSFQSKKINCSQVKCIKFSNNNKYILCSTGDGTILILDAYLLDTICELSDFSNQGSMIEAGFTPDCNYVISGSETGTIHIWSLPLGTQIARLEGHQKRCKVVKFCPTHFLMASGCRNLVLWIPDQFSIDSMNNNNSRRSLMSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T196</accession>
    <name>A0A8S1T196_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0130231</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T196"/>
    <dbReference type="OrthoDB" id="292043at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="391"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="424"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8144442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="467" mass="54943" checksum="BC1D95B60E8D96CC" modified="2022-10-12" version="1">MSEIEELTKQKQVLQSQQAILENWIILKKNLQQKFKNPSEAFKGLVKENAQELQIQDFADYAKGLNLNEIFKDSHLNEETFTKIWEQWEFNYKANQHKLQMIDEKLEVLTILEDKDAKMFKGDPQKKKLIVSQIQGCENLEDLYKKLGEMVQNSTDQKQKDEVVPNESDIFSQFLAKKSQIQFNELSISKMQTKLAEIPQQSDQSDSAQKQKLEQPLVESQIIVIGKVTLPKKSYLDSQHSIIHKRGNYSTNQQSKTFIYDSTNIKILSPKLTRPKEQFRSPKNFQQPSNFDENDQIRRSQKTKRLKGDLQNYIGEIFHYDNHQKNPYEVQQKGLQGFNCKKSLKTVQHRIDLRPTESIESQNIQKRVQNIRNKLDEYSNKRIRRYSPQKPYTSGMLIKNDTTPERRYSQRRSSPYTPGNVHRLNVDRLKQEKETVQQQNSDKKPFVKEEDEVPQKGLDRQKAFISD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T4W6</accession>
    <name>A0A8S1T4W6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0160381</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T4W6"/>
    <dbReference type="OrthoDB" id="289559at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000683">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="126"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="426"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8147420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="477" mass="56106" checksum="A9A49B3ABA307829" modified="2022-10-12" version="1">MKKSTCSLSDNSKTSFFNDGNQLFTTLPYENPQFQDQGIFQMTYQKKITIVTIYMSDTILKISDEKQDKYIDIENMCLRGVINQKTKQYGFRISKNGQYAEFFGIHLELLNKIKLYMIQPDFQSKYKVLNKLGNGYLSQVYKVQNNINGQEFAVKIYEKQNLIKSNNYERELCMNEIRILRQIDHKGLLKLYEIFEGEQNIYIVTELLEGGPIKKYINNQDLSDQEMIKIMKSLFVSLNYLHKQNIYHTDIRFDNILLRDPQDLETVCFINFGKAVQIPQKKRTQNPVTFQEEGALKFYMKKDIYSLSIIMLSILTKKFYQENQLVDELLMKNFKNISYIEYMELSPPFQRFFEMIFTEKYQKQIDSLSCENILELEVFKLLNKPRNSKILISQQLANVFARRTSKNAPRLSQREKLEQSLNIIKLPPINGDSPTSTDKTYSASPQSDRFISNQLTNILKAKHLNKRKNALKLNIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T7I4</accession>
    <name>A0A8S1T7I4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0160332</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T7I4"/>
    <dbReference type="OrthoDB" id="203073at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8147346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="352" mass="40544" checksum="2328CFB79F2A95D1" modified="2022-10-12" version="1">MGTCCTNTTQEKESFTAITAPIYINNENLEIGMQTKIKESNETPSSPIRSEKIDEENYDSIKSYRGVLINNVNVYLHSLIAQIFDEQTSLAIKIFNSLGPFPFPIIKDQTLTQVGPVQIDEGMIYQGQWLDSRRHGFGKQLYSFGSIYEGEWNNDQQHGYGRMVLPNGYYYEGQWINDKAWGVGKYVMIDGTTYNGDWVEDKQHGKGIEQWNNGQRYEGTYQNGQKTGYGIFEWPDGSKYEGELLDGMPHGNGEYCWKDGKKYKGEWMLNQMNGYGIYTWPDGKIYKGNFERDQRNGYGELDWSDGRIYKGQWKNGKQNGEGTFTYNNKVRKGVWQNGQPIKWGQSEILSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TK83</accession>
    <name>A0A8S1TK83_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small-subunit processome Utp12 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0220031</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat PWP2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8151642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TK83"/>
    <dbReference type="OrthoDB" id="3142434at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027145">
        <property type="entry name" value="PWP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007148">
        <property type="entry name" value="SSU_processome_Utp12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19858:SF0">
        <property type="entry name" value="PERIODIC TRYPTOPHAN PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19858">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04003">
        <property type="entry name" value="Utp12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="484"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="Small-subunit processome Utp12" evidence="5">
        <location>
            <begin position="759"/>
            <end position="864"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8151642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="866" mass="99017" checksum="C0F7E40298A3CDB2" modified="2022-10-12" version="1">MITQFEEQSLLTSPTTSKILQFTSDNILICGSGNRLMEYNLYNNTVKPIIQTRYNVKYFSVHNQQYYSSVDDQYNILCNGKHIKSKNQQIKQIKHSPDGKMIAIAYLNKLIIYTLQMEKIKVHSNKHKINGICWSPDSRFILSWGDDNSVIINNVFKLRLYTDILLQAHKFKIASAFFLGTNVQYIMTIDVLGKVCLWKFVTDYVTEQYEARQKFQKTKAQHHLGVDNDQSEYSIEELKYMSDFEKNVHKGRFILEKKINIKEKLVKHFAAIKSVAYHSSNMMIVGMKNGVLTLFKIEPSEDPFLIQIQSFQITQTQIDNLAINKNGNWIALGMRNTGQLIVWEWRSQSYILNQQSLSYETTCCTISQDLTTIAVGTLGGIIRLYDQTSNFCIAKFNDQHNSKVTGIKYCPSKQGVLISCSLDGTVRAYDTIRLKCFRVMQPEILNQLQCLDVEPSGDLICVGGFDPYEIYVFSLQTGQLVEVISGHQAPITSIKFIQAEEKVLLISGSWDKTIRIHDLYARGMKEGSGGDSMLHNSEVTALATRDNQIAVATMGGELVIWDVNESLILFTFDVHRDVQGGRISRETLAAQKNQNLKYFVTIEFSKDGQYLIGGGNSKFICLYDMKYRILIRRFIISNNYSLDGMKLKVNFKTIDQENKNIEESDDERQKDNLPGAKTFDVSKRSSKRVPVSVFNLQFTESNREFIVSSSEGVSIFGSAKRRQYFDPLDIDETVSLEEIQKQINQQNYIEALILSLKLNEASILDQVFNSIPVDSIITTVPAIPESLIVRLLETIIRSMQINRSVESCLIWIKQILIDFAPLIKGSTANKKVQNMLQEVLKQIRLDFNRIKNPIRKCINILEYLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TL20</accession>
    <name>A0A8S1TL20_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0230046</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TL20"/>
    <dbReference type="OrthoDB" id="93876at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12431:SF14">
        <property type="entry name" value="LD15323P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12431">
        <property type="entry name" value="SORTING NEXIN 17 AND 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="1">
        <location>
            <begin position="55"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8152427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="591" mass="70515" checksum="DDE0FD28A8E351D0" modified="2022-10-12" version="1">MKQILSQSFTQEVLPTLFREQSERPILGQKQQVQKEKQGKDLYALIVDQQMIKQIEIVEIFKEEDHESIYKKHIVNYVIQIKTDYFDYTIPKRYSEFEKLHEQLHSYLKTMPKFPQKTLIQTNSRKIIAQRQDMLQQFLQHLLKHLDHSLYIFLDFLNIKEQFQKGGFDQNVKLHQFSRDENALKDLLDTSPKKIQPKNEPEQMIIHYLKKLNEKIDERGKLFQKMEKYCFGKIQLVSPTMLKVLFIGDDERELDGIMQALSIQNNDIQSHISCINGFQFFRKVLEYDYNPNAEQALKQFGEITIKQFKKIKIQSHICGQQKNCKQDSLILLHKYINQNQLKDQMIKYLIDDDECLQEYEYFVQTHINKMIDEIKVNKDSNENLQEFLPTKSNSTEHAILEQESTIEQLYEISQMNLNWKFVQEFENHFMYHLSGQFVKTIHPLNCSIDKAIIIFTDLKIQWFHGMVQKDILEQYDQFKSLIVEYYVWEDKSTFKKMAFISEQEIIKNNDLLYQITIVPSPKPLPNHYKIKCDQKGQKMSLIFLKKKTDDSTEIIVYQNIMDNTFRTALTPVILKEIPWFNHTITKLKALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TMF4</accession>
    <name>A0A8S1TMF4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0240057</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TMF4"/>
    <dbReference type="OrthoDB" id="331948at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="8">
        <location>
            <begin position="95"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8153153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="307" mass="36624" checksum="C32513A4EDC50F7C" modified="2022-10-12" version="1">MLQEKINNGKQKSKKNYLGLGLVVLVQFLLIWGLYTAFIYQNQNSLSNCLRNLAIALWLISEICYIRTQFSDPGEILQKTVPLKLLHDQQLLQYYQNICNKCNSWKPPRAHHCRRCNKCIFKMDHHCEWTNNCIGALNQKYFVLFLFYVFCYIITIFSIHIIGIYSYIIRDQQNIWNLITNNKFKIFEVFLFIILCTFFSFFVISMLWDQIQVIRDNQTVVESLQGKFGRQQSFFKNMKQFMGNQKWYYWILPTTPILKLNYAEIVYGEQLVNLGTSYLEDIIYDEQNPKSAYFAEFMVDNYKKKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TPN4</accession>
    <name>A0A8S1TPN4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0250116</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8153958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TPN4"/>
    <dbReference type="OrthoDB" id="306901at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8153958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="60" mass="7132" checksum="7392EE12663BDF68" modified="2022-10-12" version="1">MNKIIDKKESVMLDPVMVLKNWEYLKDHLVMEDLVKQSKNENEEEDLDTTIIICNSDYDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TS88</accession>
    <name>A0A8S1TS88_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calmodulin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0250344</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8154382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TS88"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000003">
        <property type="entry name" value="Calmodulin A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="6"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="117"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8154382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="159" mass="18065" checksum="5C6E5B88B278CCED" modified="2022-10-12" version="1">MNLSEEDIEQCQRAFNDLDDRGEGEIMADDLEIALEMVGLKQKPHKVHKLISEIDDGNRGRIKFKEFLGLYAKLKYAGLQDDDQDMIDAFVAMGGNEDTTGNVDAEKLIRIIKNEFELTIDIEGLIKEVDTDGSGVIEFGEFKELLKTNYLQEDENDYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TUA9</accession>
    <name>A0A8S1TUA9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0270220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TUA9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8155643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="241" mass="28039" checksum="C33D3F2186A733FF" modified="2022-10-12" version="1">MKKHPIEQIHSSVYDKVVKSLTIFFMLISLLISIGLDIVHGVVDFYSNEDQYQAQNATIGYISDNLIFKPIKQVYFRDLDCDLSEEIQQLFPFAEYNITRWFSQLICVTRFVDIRLSTECSDTEYNCGPYCIQTSEGSQCPISKLTIIQNEIVDEDADQNLESNRDLYIQRENSSYGIFKIQVLIRGYPCLNPLIYITAGLTNNSEYIDPENCESSDFDTDIHDLIFNQQQLLYYEQDQFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TUV6</accession>
    <name>A0A8S1TUV6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0280034</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8155908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TUV6"/>
    <dbReference type="OrthoDB" id="318892at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8155908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="203" mass="23603" checksum="D5B71C2206B8C3E9" modified="2022-10-12" version="1">MQAKGDTTIDMEMMMESTEHLHNLKSDSHKDQITDQSGENQSLNQKISQIDLEFQKLLCQLIESKLSQNTISQVHDLLKRLSSSLILEIPKGPQILNSLYQIILVRKSTIKRNFPFSPLSSVLSRIKKLIKKVKQQILKRLNLFQKSKDHQINHLSSFHLYKSQCKRTDDQTESLTRQLSFEQYKEPIYKQITIIQGQGLIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TV08</accession>
    <name>A0A8S1TV08_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0270040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TV08"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8155337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="41" mass="4972" checksum="AA7B6C20E87A238D" modified="2022-10-12" version="1">MAHQKQNNMTYKTQIEKLHTTSISYLWHTQQNMLLESYSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U740</accession>
    <name>A0A8S1U740_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Inhibitor of apoptosis-promoting Bax1 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0340046</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8159892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U740"/>
    <dbReference type="OrthoDB" id="7933078at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006214">
        <property type="entry name" value="Bax_inhibitor_1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291">
        <property type="entry name" value="BAX INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291:SF47">
        <property type="entry name" value="TRANSMEMBRANE BAX INHIBITOR MOTIF CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01027">
        <property type="entry name" value="Bax1-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="161"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="192"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="283"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8159892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="309" mass="35985" checksum="14F7D5F45FE228AD" modified="2022-10-12" version="1">MYNNSNQDYYQYNQNNQQQYQQQQYQQQQQQYYNPQAFDTQDGKVQKMNDNYGYLADFQDAYIRADFVKKVYTLLTLELLITLVMIALGLYTGMAFWLVQLEGGFLTECFYSGYGPIICNTGYYLYPAPTWLFYVSFFVALILQCMLYCGGNIARKAPTNYVVLLLYIIFFGFTLTTFCILMAIYWGQAIVWQAWGITFVIVFALTLYACKTKTDFSFKIGAIFILCPTILMLVIMSCIWWSAVIYILLCTLFIVFYGFYLIWETQLIMGKGKLKLSIDDYVIGSLLLYATIIQLFLRIIEILAIARGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U7V6</accession>
    <name>A0A8S1U7V6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">40S ribosomal protein S14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0350228</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U7V6"/>
    <dbReference type="OrthoDB" id="308517at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.80:FF:000002">
        <property type="entry name" value="40S ribosomal protein S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_uS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_uS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018102">
        <property type="entry name" value="Ribosomal_uS11_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007176">
        <property type="entry name" value="PRK09607.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S14/30S RIBOSOMAL PROTEIN S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002131">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00054">
        <property type="entry name" value="RIBOSOMAL_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8160900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="168" mass="18212" checksum="3360B80186CCE53A" modified="2022-10-12" version="1">MADNKVQPAVQQTKAKKGKKNQGDDQPKEVVATLGPNVAGNELVFGVAHILSTWNDTFIHITDLTGRETLARVTGGMKVKSDREESSPYAAMQAAKDVYEKLKTLKINALHIKLRARGGVDTRQPGPGAQAALRALARLGLKIGRIEDVTPIPTDSTRRPGGRRGRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U8T6</accession>
    <name>A0A8S1U8T6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA mismatch repair proteins mutS family domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0360086</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U8T6"/>
    <dbReference type="OrthoDB" id="295033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032301">
        <property type="term" value="C:MutSalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF148">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="5">
        <location>
            <begin position="636"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="355"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8161270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="707" mass="83082" checksum="7EA06488528C9026" modified="2022-10-12" version="1">MKTKKLNCFDVELCDNALFDNEQLIRPKSKPKSNSELTPLEVQYQEIKKDYQQDLVLLQRGNFFLAYQEDAHFIRKYIDLQWKIPNYLVGLNEASVIEHIHTLIMQTDRKVVYCEQSVFQMTQNQIRDRFITGIYSKGLLGFGILGENIENIETNYLVSLYKDEAIVLDTISKVAYKTSIENLNFIKITEIITNEKIKTLALKNTAKGDSAFEQLEIFFKERFINGISQITFIDDSSLFSKYCLISDNAIKQLFFKPLIQPSTKEGCRKLQMILRKIPIEGIKDKQLAQQDMRNHLIFRDSFLGEIKRVGSLQHQMARVFNAPKDCLILKQFLNNIKYILMQLKQKIYINKQNYKSEQLKNLKELNDNILQKIQQEFIWEGENLDILVPQNQERTLYNQILENLEKTEDEANDEYQKIKSQFYDNDPKIKYYGFEIEMPEDYKTPSFMLFSNKRQGYRKYTTQLLQKKQQQHIKLLDQLKKFIHLFYQQVIENIIKHKDYFQEIINQISDLDVLIAMSKYLENDQHKCLPIQSDQNQLILKDFCHPLIQNCIPNDLNINKCIILTGYNGAGKSTILRSVGLLVILAMLGCYVPCQQMIFKTFNEIHCRMGAYDTIRESTFFVELQEVRRMIDTQNSLLLIDELGRGTSSIDGHAIAYSILKYLLKQNSMILFTTHFDITLDQVMYYMDNYQLQKGQVIKELTSKILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U9F9</accession>
    <name>A0A8S1U9F9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0360125</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="296318at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8161348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1593" mass="186450" checksum="D1C870DE99DE3DC9" modified="2022-10-12" version="1">MSHFARLVELLNESPKNQQNSELIQLVQILIQFQFPQHTYQMICNSFSLLIAKTGNEQIKQVVEALLQYINKKGSLKSIALNLLVTLIQLTKYEIQSFSYTLTQDITTTLMKLLKSSDYRILLCNVLYFLIESQKIEILKIALKFAQNEKLEILQQTGMQTLIYLMQEEVISEQMYLIAQKSDDSPLFYKYFSRYLFQQQNIVALTKKDKLKSQVQLQPREQAQKIFQLFDENLSKFPSNIVGNIFIAYKKYLLHEFKKLGLSIVRGSFEISFSYLGSMALDDPYLHTVYKKTVNLCLYMVQQVQLNERKDLLEYFFDKISKIKTQKWNVYNYKLLLKGINFLFDTLGSNVFDDKRSKPSEFIDILTPIFKINDIFQHPMSIDKPICISVSRNLFNTFESLFQENKKWQQSILSALLNYAKITLAEINVKETNQLLNQLRVQCALLSKLFSLLDLQLHSISSELINHLWETAKSFLNKSQQLRRTGWIIIAELLMVPYFGTTNKVKEIGILVEKNLTSKEDIKDEQQLLSYCSVGLVIISILNSNTLSKQIKDTTRKFFAQQTILMFNKLQQCKFDKQSPFVIASKYIFYQAIRQIIPKYYSNELNTLVQFCLEDIINGNSIWNQVNVHKNNHYVDELLGIPRSEFQLVLAASKFLGHIISSQSFTLKNKINFIRYVNGTLQTQVINIKEVQQKQSKQQCYLFCLYQIALKIYQANTLVSQEFNEQIFQVINICQCVDNQIQQLCAYLYCSMLQIKDVDQAEQLNQLLSQKSQANYTYLVLEILKKKLTGVQLQVQNLLMKVLSQMTTDVAKVIQLFQQIDPSLLKNVWVFLVFAISQSDKPIQITQFKILSGIILNYVRNGGVKDNYFNIIYQECRCRLQSKQFQILQLDFALEQDTEQTVTKAINLLLKKPSLKIAVFIDQLTDKYNMPSLVQPILSVLNNLFSNQRVHSTNEEMELRNLMKNIFIKVYNQPIAELLQLLQPYILDEVIPENQDRIPIHIKTRIFLLKRLQKLFKQCDTSNQVFDFLFKITTSIYEEAKEISFDMFQHFFYKEKDLLNFAAQIDSILCDTIRRENQNPPSVNLTNMKLISKFAKIVQDEFLATKLIQLLSKPLLNDGRKKLVPSQFFSEKSQLNILIQRIVSLGSIYRKIPNYFDQSLVDQLKCYVEELLEDIFVILCTPRSNVREYQSYNFVYNGKKYSYDQVFLHQSLPKLLRISNLDKPIHFTLIGYIITQYGAFEQNENMFKKTIESLTKERILQTRVYRIKGIQLLTEVINKNPQNQLLISGIQCLLPIISAEAPYSLVVCQQFYQTLIHHQIDLQLIVEPIIQIYQLTVDQNVKLNCLASLLKISQQNNQTLDQINIIFWDFLLTQDYDFMRCTQLIQFYKDKIYSIKFSGYIQTILQSISNTQKMRQEDYYLLLNLDGLINNKLDNQGYYLIIDKHFTNLLSISLSLIKQIKQNIQTILNCMLPAIYHKIVNKESETYLFIGKFLLLTYLLSNQKQLLFQTIFRMMDLFTDDTLDSSIIEASKQNLKIIVMNTQLNQMRLPDDLSEKVQIIYDDIQREIQEKQQREAKQRQPQQSGQIVLKMFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UAD6</accession>
    <name>A0A8S1UAD6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ERV/ALR sulfhydryl oxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0360078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UAD6"/>
    <dbReference type="OrthoDB" id="17199at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016972">
        <property type="term" value="F:thiol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017905">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxidase"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51324">
        <property type="entry name" value="ERV_ALR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERV/ALR sulfhydryl oxidase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8161254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="41" mass="4937" checksum="E7303F520EFF5246" modified="2022-10-12" version="1">MLFFNVRRQFFPCKECAGHFLNMITVLPYEGTTRVQFMSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UBI2</accession>
    <name>A0A8S1UBI2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0340095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8159986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UBI2"/>
    <dbReference type="OrthoDB" id="311339at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="361"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="378"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="397"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="415"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="439"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="457"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8159986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="468" mass="50571" checksum="315BACECDD3C50B8" modified="2022-10-12" version="1">MGTQQQKAPQTKQITQFQIEIACLKVKGYVELNRDRRTNQALMKEKALNEMLRSPSRVMVEEYQKFQQLVQDVRFIETCNIVLRYCDIVKDQSIRIFRCGGDHSKIADLMPYIESILFAADNLNLEQVMEFKDLMIYYFGPGFNDTNKLINVDQDLKKLYKTPLPDAYEVNEFALKFAEKYGFSEEQLNASGHQFSSKILKPAQGGILVDQQALGQQQFMPYSNQGFGVPFGSHPESFSQPGNQGFGDFGTQGGFGQPPIGGFGNPPQGGFGQPPIGGFGQPPTGGFGQPPTGGFGNPPQGGFGQPPIGGFGNPPQGGFGNPGTGGFGQSQPGGFGQSQPGGFGNQTQGNFGAQGGFGTQPQQNGLYNQSSNQFGNPPAGGLGTTGGNGQANGSSNPYSCLNQGPQIMASQSMKVPQQYQNPQQPFQPQVQPVIPPQQFSGQQQKNNNPNSQALDDLEARLRDLDKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UGA2</accession>
    <name>A0A8S1UGA2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0380245</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UGA2"/>
    <dbReference type="OrthoDB" id="302055at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8162799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="294" mass="35191" checksum="F2E596045325A67D" modified="2022-10-12" version="1">MQNSQEQLNNQKMLRKSIQKNKPQQKCIKKDVGKPAGIDVIKLETPKVWSCLNVERQLIEYFTQIGRHVYLSLAGKIKNSQKDGLSDKALAKLQKYQAIYQNKSIYSYIEHIAQSLFNWKCQKLSVVYFFRNDRTSYNKYQKIYLINEKWNQIIKNIKFLLNEQFYFELISILKMFESNYTTEQICDKMIEQSKELDQVPQIIQNFFILLNKEKKIEQQIHGASCAQLEMLKTIYLNSLDNHKDYLQIIIEKLRQIMYENWIPQFIQQYDNVSLEVQNKIFNDESNFIFQSDKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UGY4</accession>
    <name>A0A8S1UGY4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0410024</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UGY4"/>
    <dbReference type="OrthoDB" id="10463842at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8164421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="240" mass="27892" checksum="3E703E0566CDEF90" modified="2022-10-12" version="1">MEKVNKAWNFFVQNLSFKSNPEQVKVSSETVQKEDINSSIDCALGFSNSLLHNNSSSSGMLSSSSSQYPIVINFIILAKICSQFLPKFSSIQKDNERKPIIIPYQDDHKQLFKEKNIKNVYQCKRENKYSLKQYPMAKLITEHTDTIICSDNTCMLASFHENMHRVDQGIYKKGQASQVYVECYEYLPTKQIQFSDAQDIIDELKKERYELFNNNCQHSSNKIIQKLDLKFNDSINLERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UMD0</accession>
    <name>A0A8S1UMD0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0410270</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNase II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UMD0"/>
    <dbReference type="OrthoDB" id="10261598at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004531">
        <property type="term" value="F:deoxyribonuclease II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09120">
        <property type="entry name" value="PLDc_DNaseII_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004947">
        <property type="entry name" value="DNase_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10858">
        <property type="entry name" value="DEOXYRIBONUCLEASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10858:SF23">
        <property type="entry name" value="DEOXYRIBONUCLEASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03265">
        <property type="entry name" value="DNase_II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035847044" evidence="4">
        <location>
            <begin position="20"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="369"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="369"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="385"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="421"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8164889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="479" mass="55923" checksum="ABC6BB0E3BEB12C4" modified="2022-10-12" version="1">MHIILQCLVLAQLILITLSQSCLNSKGQEVDWWFILKMPKNSKQKWTGLEYFYCDKENDCKEFNLQDDDLDSATSPLMQTIKTIDFTDDKFISILWSDQPNSEITYSNHAHSKGIMRAGLEDKKNAFIISHSTPRFPKLNEYGQIDDKVEDNFARNGQHFLCLSTTTDKIDEILAESYYEAEIQIYKVSQTPTKIFGRGYYPNIYKLWSTKKPGKGESHHRYSKTTLYSHVRFTTRENQKFKIFSKNEDFEQDFYAKFVAEELQQDLIMETWVRQKGKGLDDPVCKKYKTSSNHQVSFHGKNKHKEVTFTYLYTKDHSKYGITLPRDPASTSKGESFITTDYSHPSLKGQIFTSVKNLQRSKILNVDEDTVIKEDEDEEEDEKMEDASPKPDDSMDVDHPKSEEKLLGRKRKRDDSEELEPPPKRMPTEKKNKDKYVCVSDLNRQESQWFRGGVVYCFYNENLWGHIDKAFVGKQLCKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UPK3</accession>
    <name>A0A8S1UPK3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ketoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0430068</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8165672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UPK3"/>
    <dbReference type="OrthoDB" id="421309at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05353">
        <property type="entry name" value="hydroxyacyl-CoA-like_DH_SDR_c-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051687">
        <property type="entry name" value="Peroxisomal_Beta-Oxidation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45024">
        <property type="entry name" value="DEHYDROGENASES, SHORT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45024:SF2">
        <property type="entry name" value="SCP2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="3">
        <location>
            <begin position="9"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8165672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="310" mass="33684" checksum="9560C1482443E781" modified="2022-10-12" version="1">MSDLRFDNKVVVITGAGRGLGKAYALFYGSVGAKVLVNDNGCDLDGKNTNPKFADEVVATIKSKGGIAVANYDSVLNGDKIIAHAIKEFGRLDVLINNAGILRDKILAKLTEEDWDIVIKTHLYGTYSVTKAAWPIMRDQGFGRIVNTSSGSGLYGTLGQTNYAAAKAGIVGLTLTLAKEGERRNILCNVLVPVGASRMTETIMPPDVMSGIDPNNLTPILAVLTHESNKEYNGGIYECSGGFFSRVRWQRSEGVSLDLPVKISEVQKNWAKINDFNGKNEYPTGNADLFNKVQENLERLEQQAAQKPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UT20</accession>
    <name>A0A8S1UT20_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0460038</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8166729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UT20"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8166729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="40" mass="4947" checksum="8E682B92ED30E0DA" modified="2022-10-12" version="1">MKEDHFKLKKIVQKVKEMMILVFHQEENKLLMKNMFVQVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UTN6</accession>
    <name>A0A8S1UTN6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0480090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000048">
        <property type="protein sequence ID" value="CAD8167905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UTN6"/>
    <dbReference type="OrthoDB" id="304153at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8167905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="465" mass="54167" checksum="BE2ECCCE8056F561" modified="2022-10-12" version="1">MNNQLHNLNCKFCKINQNKFIQKQIHEKYFEQFNFYFAKPITEILANVPVDHVILFKDQLYLHDENEYMKRFYKREEQDPRIKLLSDFYYEQNKMPQPNLCKVNAHKFMEKRINKLLKLQQKAQNNQNQQPITKKMIPDHIFSENYVESVSDSYPKEQISWDSKSHNIYDISQTALQQSQSKNKTNVQLPILNISNDQNKLYNCENDQDIDNIICEINTQTSRQLSSITKIPFKKKPSSSTNKKKNQSIQQPQQPKIPIPPLKLQESAKSSATTVPNNKSTSIQSLMKKYDQILQKRFQVKEILTERNEIRNAAAFGSSCGIATNRGDVGSSKKQITDEMLLKLISNMKKSKNKSDNYQFHASYKSVVSHPQTGPAQSCTFEKENEKKKKYQVYLPKQIKKNPSQMSSPTTVQNTCSPSMLLRSLSNPSMAPSPIRRLEIKVNLNKLIQQQEQQIGYWTQRSKVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V0Q0</accession>
    <name>A0A8S1V0Q0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0520140</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000052">
        <property type="protein sequence ID" value="CAD8170093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="283111at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051175">
        <property type="entry name" value="CLK_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE DOA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646:SF11">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="39"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8170093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="194" mass="23085" checksum="769E131FF78F6157" modified="2022-10-12" version="1">MEWSRSREREKIKKQREKEETKKEKHYKLQKDEIFNQRYLFVQAISDGTFGRVVRVFDGGENDFKAIKIIKSVKRHIQSAKIEYGILRTIHQQNQYHPGNEKIVRAYEAFSHKDNYCIVFEDLGLSLYDFMRGKYFGGFELKAVQEILYDCLQGLDFMHTSGYTHTDLKPENXLSESFQKLLNFLQNQIIVQFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V1J2</accession>
    <name>A0A8S1V1J2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0540179</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8171210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V1J2"/>
    <dbReference type="OrthoDB" id="302770at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12422">
        <property type="entry name" value="RRM2_PTBP1_hnRNPL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000566">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15592">
        <property type="entry name" value="MATRIN 3/NUCLEAR PROTEIN 220-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13893">
        <property type="entry name" value="RRM_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="259"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="102"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8171210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="454" mass="53263" checksum="52E0804418B61CCC" modified="2022-10-12" version="1">MALTKVVLLCIQNKKQLTITHDQIYKHFSQYGQIEKILIFEKTQIWKVFLETKNKETTQNLIKQCNNNILNSDSSLRMQLYPSNLENVTFSDMNSAGKDYSKFKEKRDSYNSTDDEQTSVGSQGEQYPNLNSTKNSPQNVQQSIYQYQQHLQQRLYEQQVLQMRLQTQSLDLQIPDYINTLASVQWVEQFVQLGKLLQTQYLLQYQLIDSYINYIKQESVNALSEIQNQQRQQQIQQQLQSQINTLNHIHHVDQQEKSRVIVAKSFDDSVTTLDMLYNIFSIYGNIDKMVYQKDKSTLLVEYHLQKCADQCIEKLNNVLFQGQTLQINYSILQEITFLDELEELGLVEKTFYEERFIGSEATNRYKPKNSFIAAAPNDTIFLMNLSNELLNIESLQPLLETDIVDYKFYEDPKQKHSCALKFKTVEDAMYFLAKNHGKIMSDRKILMTFSKKSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V3I7</accession>
    <name>A0A8S1V3I7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0540068</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8171013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V3I7"/>
    <dbReference type="OrthoDB" id="10249433at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000117">
        <property type="entry name" value="Monoglyceride lipase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051044">
        <property type="entry name" value="MAG_DAG_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11614">
        <property type="entry name" value="PHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="2">
        <location>
            <begin position="73"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8171013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="384" mass="44570" checksum="B98A79E2CEECCD2D" modified="2022-10-12" version="1">MSYSTIDERLMDSPKRTESTDIQSDSQMLKKGFNPSFLGIQQTSKITVSRRYLDTKTHGVQLYYQEFTPQFVDAQVIIVHGFGEHSGNYQQLTDCFILNNFKVYLYDQRGFGYSGGIRGQATVEQMHMDLDTVLQLVDRSIPLFIFCHALGASMIISFCLMNPSFQFQGLICSSAQLRVPAKYGKIKMLTLKLMTKLCPELQVNTYQNLSYASKNNHHIKKLATDHLMHPYMSIQFAYNVLLFQQFILPNATKFKIPILLLHGKEDKVASHLDSLDFYRLIQSKEKTLKIFEQGFHELHNDSEWSKMKIIITQWCTKMLSKDIKMSFMREHNYGVIVKQYRSKIRIIITILLVLFRKKYACLKASTKVSIIIFIELLLRVFISN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VDY9</accession>
    <name>A0A8S1VDY9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0600182</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000060">
        <property type="protein sequence ID" value="CAD8174012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VDY9"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08215">
        <property type="entry name" value="STKc_Nek"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000097">
        <property type="entry name" value="Probable serine/threonine-protein kinase nek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="4"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8174012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="365" mass="43374" checksum="A00457FAB8E70236" modified="2022-10-12" version="1">MEYYRKIELKGKGSYGQAILVQNKQDRKFYIMKIIDASKFDIKEKENALNEIDVLKNLHHPCIIEYRESFVDKNKYLCIVMDYAEEGTLYQRLEQQKQRQEHLKESQIIEWFTQICLAVKYIHDRRIIHRDIKTQNIFISKGEIKLGDFGIAKSLINTEDLCQTAIGTPYYISPEVCQRIPYDYKSDIWSLGCMLYEMMALKHAFEAKTMEGLFLKIISGKYQPIPINYSQELIQLLKDILNTDPQKRLNINQILDYRVVRKSKKEFIYRKTQNIHKIQFISGQINKNESHFGKIRYYQSEQKFNGESLADQIEKMRMDLEEQLGVEKFILIYNSLKNEEVPSKFCNKIIIDKINQLLELEQLIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VEI8</accession>
    <name>A0A8S1VEI8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">folate gamma-glutamyl hydrolase</fullName>
            <ecNumber evidence="3">3.4.19.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0590021</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n) + (n-1) H2O = (6S)-5,6,7,8-tetrahydrofolate + (n-1) L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:56784"/>
            <dbReference type="Rhea" id="RHEA-COMP:14738"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:141005"/>
            <dbReference type="EC" id="3.4.19.9"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.9" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDO010000059">
        <property type="protein sequence ID" value="CAD8173256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VEI8"/>
    <dbReference type="OrthoDB" id="64220at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034722">
        <property type="term" value="F:gamma-glutamyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046900">
        <property type="term" value="P:tetrahydrofolylpolyglutamate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000054">
        <property type="entry name" value="Folate gamma-glutamyl hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015527">
        <property type="entry name" value="Pept_C26_g-glut_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011697">
        <property type="entry name" value="Peptidase_C26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11315:SF0">
        <property type="entry name" value="FOLATE GAMMA-GLUTAMYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11315">
        <property type="entry name" value="PROTEASE FAMILY C26 GAMMA-GLUTAMYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07722">
        <property type="entry name" value="Peptidase_C26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51275">
        <property type="entry name" value="PEPTIDASE_C26_GGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035788739" description="folate gamma-glutamyl hydrolase" evidence="4">
        <location>
            <begin position="17"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2 3">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR615527-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8173256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="340" mass="39615" checksum="2C718C243D6D3735" modified="2022-10-12" version="1">MLRIIITSLFISSVISVTKPTIGILTNPSDLKDYDKSLYSYFPSSYVKWIEQAGARVIPIHWDSSYDEITIILNQINGVLFTGGDVDLYLNNTQPGFTFNKFTDTASFIFQKVIQFNKAGKFYPLLGICQGFQLINYIASSYYEVLTRMNDDLGKQRLLEIVPEEDSFVLKSIDKPILDYLKNVDGPYYSHNWGVVQQTYEKAYSLGAFFKITAYSRDGVNQKYIAICEGREVPIYGYQFHPEKHQYEWITKATHDVQHITYSQQLAMDFIGMARKNDNSISDEELAQLIIYNYKQINRMEIPNTSFSQVYLFDRNRNETKSDQQQLGIYQTIKYFRKRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VGD5</accession>
    <name>A0A8S1VGD5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0630093</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VGD5"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="43"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8175513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="523" mass="60942" checksum="DDCBD30D23533FD8" modified="2022-10-12" version="1">MFQKDSLQDRLKCKIEFTQDEEKVEIISLKSSIDDTIRFEDKYEQQEFLGQGAHAIVKLAKRKGTNDIFAVKIMRMNNEEVYNNVKRTFFNSRCLRHQNIIQEYELYINEKYYTANVVMEYCPYPSLEQILKDRGILKEEEVRKIIKQLLMAVGHIHSKGICHRDIKPDNILVNIIGNCELKLLDFGVSSRFIWKNQTYQMMTKTGNIYYCAPEIYHQSNYSKEIDLWSIGVIMFQCLTGELPLQNENMSDHIELLGKPEMWNFKNKIKGLSLNAQNMISRLLLNDPNKRIGYLDALNHPFIEKNRTYTTLSMLQSIQMIDDEEEDHDSRDEIFLKKCQSLQTSCSVNQQSNIHRALKTLPLGQEYQQIVLEDLMLELENVHIIQKSNSDKFTGFIQFVNSLGNSQGVTISKYIEVNTINQCSLGIGSTQSIESSDHNIQDYFEELDDIQQSDVFQNDIKENNFQQNFYTQNSFINQVRTNIEKSLEVDYQQDTGLNNKINSIISTLDALGMNQFDETIEDQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VHI6</accession>
    <name>A0A8S1VHI6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0620232</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8175219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VHI6"/>
    <dbReference type="OrthoDB" id="668540at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010608">
        <property type="term" value="P:post-transcriptional regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF12">
        <property type="entry name" value="MATERNAL PROTEIN PUMILIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PUM-HD" evidence="3">
        <location>
            <begin position="122"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="184"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="220"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="363"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="402"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8175219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="475" mass="55651" checksum="975279804AC28188" modified="2022-10-12" version="1">MTDDDFETEQNLLGSILSITDEYEYQDQKKLNASDECNQTQTQIKLQRQYKFQTTDTFNKSFEQISLGSDQICSPIQPEGKLKYKNSGVCTSFSSDNQTSLENSMIFGNVRCFDYFQKQSDQFSPIYYSQNNPIWPQYQKSNQILDIQLDIDIENMCGNQVLSRKVQKIFETGQSNQRQQIFCKVLVNCQEFSKDIFGNYLIQKILEKGTTQQQMQIFKQLLPYVIELSKNNFGCRVIQKLIDIISKNDQLIVSFIQEIKQNSKCLLIDQNGKYVILKCLETLSIDIFRFILKPTEELGIHMCDSQYGCKIIQKLIDNYPTQVDTLIENCISNQHLLYKSQYGNYIIQYAIKQSRYLDIIANYILNNLENLCFNKYASNTVETILEYLTPKLKNNFVQILMKLSDKNGMFIFLNISTNPFGNYVIKKLLQIFDQEHKQQLLNLTKQNQHLLYYIKQSEYGQRIYILLTTQLNDQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VHU2</accession>
    <name>A0A8S1VHU2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0670209</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000067">
        <property type="protein sequence ID" value="CAD8177438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VHU2"/>
    <dbReference type="OrthoDB" id="1668230at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27001">
        <property type="entry name" value="OS01G0253100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27001:SF931">
        <property type="entry name" value="OS11G0664100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="497"/>
            <end position="767"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8177438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="767" mass="91149" checksum="9B864BCA65C9AA69" modified="2022-10-12" version="1">MEPQIKEFVSTLLKTQITDNLTLLDKEKKLNRQTGLICFLQGYCLEIGFQVQPNMQQAISHYLYGIKYYADPLCAHQLEQYYNKNKNKILASFCKCLYYLLIDLIYEIKHQNKKVECEKYNEAIQELAPTPDLPLNQLSNFLQALYLFRENESQQHYDSLLQNYEQMGAKIHAYPILIKQFTQQTKNPDKFPDIIYKQCQLVNPEPFLNQCLYNISLYGMIDGSHLNSIRQIVFELSFLHRPQPYAEVIEQLYTRGTQYVVHRILIFQKRAKVLKDSELSEEDIQYVASQNSLISLYILGRRQKDKSKNCLLCKAKELLKNPWPETPYIMFVYMFYQAKLELHHQTHDGYTKLLDQNLFEECKQYYDRNVLTFKQVRTLQFYSIRRRLDKQRDKITFKNKQIQAMFDQKRVQQLDYSRGNFQVLQQSQIELRRSFVNQSPEKKGIDAIKQTQSIYVNSGNKRVNFDRTIQGPQILPQLEEKQMQKSRFIDLENKIRYTKLNLIQFEDISEVQFISKSNRDGQLSLGKYQGQVISIKTLAYMNYDQINQYFKYIKKFTNLEHQNIRTIIGYNIDDLQEQDLSGQIRIITYKYDYNLRTFLQKNKISTKEKWHIAVQIIDAIYYLHQHEIIFCNLHPNNILIDGETHLPLLIDFDLQFDKEYLDIKYMAKQVIEEEMPLFTDKTDIYSIGCILLYLFFGCEFQFQQSYHSTTLTLEAFQTLNPHQSTNVDFLIPPGSEALQERARVITLKCLNGDIELLDLLQHFYDYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VLQ2</accession>
    <name>A0A8S1VLQ2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0690129</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VLQ2"/>
    <dbReference type="OrthoDB" id="306237at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8178324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="356" mass="41791" checksum="7BEC59FD44E5D59D" modified="2022-10-12" version="1">MLKSNKLTSESALTTNRPTSRIDTSQLNRGYSQYTQRQSTNKKLWIKINKVTEDVTDSKPYIAIEKQRNTNSCHIRPSLSRKTSSDIKNYIDYNEIKHFIKEVDHNYEDEPFSKKDNQKLTLQYQKEKRLNHLNLILATFRDRIKSRNISNDIESNNQQKFNNKIKIRSKQIAIIKQQSDNQIGFKISQLKQQNASQQLIYSNYLKSFRASSDYILNQIPHMPSQQISKLTDDLGNLENYKQYNIYNKSISLTSDRLENTKEYFQRPQISMKQILRRNQKNPDEIDNQLFIDESIISETEAKLNSLYGESISIQKNLLQNQKSNPIKVINAIKKMDQIKSIINNVNANKLMNKFII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VNP6</accession>
    <name>A0A8S1VNP6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0680134</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000068">
        <property type="protein sequence ID" value="CAD8177845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VNP6"/>
    <dbReference type="OrthoDB" id="341421at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10527">
        <property type="entry name" value="SET_LSMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1410.10:FF:000035">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1420.10:FF:000021">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015353">
        <property type="entry name" value="Rubisco_LSMT_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF137">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09273">
        <property type="entry name" value="Rubis-subs-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="1">
        <location>
            <begin position="18"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8177845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="449" mass="53389" checksum="E151542EA3774AAA" modified="2022-10-12" version="1">MMIHQDPYKNLIQWLKDGKAEVSKVQIEVKSEGYRTLKASQFIRQGEWVLFIPRTHYLSLEEVKKSCLINRKMIQLNYIPNNIQTYFVNHLLQENRRQNSFWKPYIDVLPKDVSGFPTYFDAEQDALLKGSPTLYTVMNQRRIFREEYDNLKEAVKEFQRYGYTYNDFIKFRILTISRSFPVYIGENEQQQLLVPLADFVNHDNNGFLQYGYSPDADGFFMQAVRNIQKGEELFYNYGQWSNKYFFMNYGFASLTNPMNQFDFDVCIDRNDRLFNMKVDLTGGNICWGNRLVNETDHDTFRQSLATVRFAQISKLDDFLKLEEDVQNYNQFWPGWHTTPKTIELEKATFKAFKELLVSELANFASTIEDDQRRLNDPSTPEFRRHIIMLTMREKQIIKKNIEVCDMMLSVIDKSSEELKNLMRYYNFREIARYVNSEIIPMKLEEEGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VT47</accession>
    <name>A0A8S1VT47_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0710021</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000071">
        <property type="protein sequence ID" value="CAD8178989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="302631at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="250"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1016"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1196"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1274"/>
            <end position="1298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1452"/>
            <end position="1476"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="925"/>
            <end position="952"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8178989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1515" mass="179414" checksum="4CA2D5FD5820CE4C" modified="2022-10-12" version="1">MNKFQDQIIKQNQKIRNTLYQNLIILGQFFYTLIWIFIAIQILGEQREDLHFKIFYYFSLPTISILFNIEQVIIQSITIIFSLTFLLTVLFGGNQKYSKKILDILWDAYPNIWQLVIIQNSGHCLLTDNTNLILGIINIILAIGNSQLTSLYTFSQNTTQKLIKSYFERSLLPFCYSVLALFAGFQINSLYLSYLYLGIQLIFDLYFGILYYVKPHMQYLNMKTQKLVVLCSFMRFPLELFLFYYQGDEILYLSLFSLPFMIQIHFILWDNRFMNLIYRTNMFFQYICLDKFREIDNLIECYIEYYFEIIDYKKPFNRVLMLSIFCNHMNMCQNLKCFCQNRNFHESVKITTQLQLDIKEIQQQINYAVAEQDIQMSFKELMLLFLDNYIKHVRYTAMGETLILKLSLYESQNFQQRLFQAKLRFQYLKIYYHKYHPQFLERWATCTLVTQKLQQYIGGIINLNFDLSLSLQYFKLLENIQTKICKVIEELLKRNYQNPVPYLLHNSTQKYFNQCDELELLIKQLIQLNGVSLEVRELQDDFYSQIKVDYGFKNIRKKTTIPFINQNQTAFVICDYEGDLVIKDYSNNFISMINVSSQIKGSAIEQIVPVILQQYHRIAISQMINTGQTKLVNKDQYTLLVKSEKYILEVDALLRFDYRQLEFLRFIGFIQLRDKDSILLLANDKGKLDSYSRSAGILLKLDDVDINSLNYCYLSPCLLEDKVEQEFDGFLLIPKRHYRSDILGYMRYFIGSPDECYIFLVNATHQKKMYTSVTIQCLKVNSFYQLKELDFKIIALDIMIRQIMILKQVNKSQYMASAESVRKIQTFKKTFKFHQTHTIEFTEGEYNLIYEDDSEREIQVQTKNQNILPEFIPSLDSIRTQNEKLLLTFEQYRDYHNTLSKDKSFEQQQKFDTLEGSKSELDTYSKQKRNTNQLIEIELNAAEDQNIEEQLQIILATVKTRQTRKELLEMKNQMKNKHKASTWHGNGNVQSQKQLIRNIVHQFQISSLSSLLSLKIVSCFLVLINVGFILGFVINSIINIAGIQMDSNNQTYPLILFQDINLVLLGIQLEDWHNEDYELAYNFKVLGAEQYQNLSIHVNDIQLDYHTFLQELDEEIIKVQENGIPIIYYKKYFLKNYMVEKLRTIQEAKSVPLFGDDSQFLFDNYPEIAQQLWNLKSKTTRDVIERIDQLDQMSQLLLILSIICNLIGILIYLTILISIIQWRNQLYSTLFLFNNIQMLITQLSDSISRQIVYTLNNKGYDEEKNKKIKLVWQIYAALTFFVLAIISLGFYIPLFLYIDSVNLQSVDVNQLQTELAMMQLKQSIILTWLSINQTEHMYNTSLIHNTTIIQYVIDECTPSEYFVLYDLPFDSKYDYFPEIQQYSTTSLCNCSLLQNQLKNGYYGFQSYVSQHLYDQDQQDEIMEAYQIVFEYQIKKKQEFDDYMQIRLKNTQLTLQIILEISVIIIIIIQTSLLLLIHRQIKKLVKSTKSIMYLFPPQILANNKYTLQVLIQGSNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VT72</accession>
    <name>A0A8S1VT72_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0720050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000072">
        <property type="protein sequence ID" value="CAD8179453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VT72"/>
    <dbReference type="OrthoDB" id="305429at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8179453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="146" mass="17621" checksum="88697D4E12E07FCC" modified="2022-10-12" version="1">MSKFKHNVEDVMNKLEQQIKENRRRTRTLSLAKQNSFNSQCRQRLNTMHHIADHFLLKTIYIQPQQDIQQRKKIVNQRLKIQQRKYYQQLFYDKTLHKNQKTEVQPFASCITLSQLNQNAIPLPIVFKQKKEIKKLTKPNSKFNLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VTW9</accession>
    <name>A0A8S1VTW9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0760051</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000076">
        <property type="protein sequence ID" value="CAD8181288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VTW9"/>
    <dbReference type="OrthoDB" id="294939at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8181288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="490" mass="57982" checksum="DA1C672B6D54DD13" modified="2022-10-12" version="1">MIRNNEILTKNDRQNSHLDSYRAKFQIAGQTILNGDSSFTQEHTNRNIYETEKTNHQSILQQIGYSVSQELNQQEYQKKTKASPVSTDFQSALKVSIKDSQQISIVPRGQSIEFFNNNDQFITTSESPYNPQNIRKTFQYCDGSYEYINDNIQSGSSDHTPKPIKSMSLSNKTISTNDIPTMIRVKFKRIRKCFQIIKALFRMKTLCNQKQSCWNLQIDILKRNQKILKYNESITIIKIKQWTQMVFSKMISIIQQKNLEKQKLNFIVKFQYQQNLQDYPQTMTSVEIDQAIVFIQNSFTFAMSNLVVMTTGKYLLNELNLQMHQDQYFEYRKQFNKFVSERANYLTKEYTQMNEQEKQLVFCECIIIHNLIPSLLKLTNSLEQLKCNIPNIEFLIRCIISLFQYFFIIHFSDYPKIEMKKQNIKYTQYQLIKNGSQLILNQNQQLNSDEFINGIIYTQEQMQLILQKESWFQKNKKKMNIISQNLGFVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VV50</accession>
    <name>A0A8S1VV50_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0730137</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000073">
        <property type="protein sequence ID" value="CAD8180065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VV50"/>
    <dbReference type="OrthoDB" id="294627at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8180065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="290" mass="33530" checksum="CF83C1745D34DE55" modified="2022-10-12" version="1">MRQQQPYFIRPPNLSSRHSTNKISFLNKEDTPSNSQGVRRKTPIIAQPLHSPTHFKDLHRINPIKRIPTLNSPLHDGSISKYKIINDLSVYKLPTTRRIKSDDQTSNIIPVKKQQSQHINNIKLRTDRRKNFTPLIKMKTEPNEIATHSISKQDESQDILFRLHKQSIPLEPKDKFASILGDKIVSSIKKIDKKTENFIELEQDSQFFLETKENKKNKEIPALSQQTKERILDLLCLSTQDLKKKFAEQSRVGNSKINTRQPNKLVIPKTGKSKFPLDFFNALLPNSILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W208</accession>
    <name>A0A8S1W208_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0800126</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="293398at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1696"/>
            <end position="1718"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1719"/>
            <end position="1750"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8183191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1763" mass="205340" checksum="890C9140776C9974" modified="2022-10-12" version="1">MLLIAMLTQTKKQKDFLFDLKQGKSIQVELPEKQDKDFIILYIRPLQDEKKSDLGEIQLKSKDEEFKLNEFYETALFTIKSEDINLKCISVPCTGRITIINTDAIELGNKNTELILTQKQPVQVISMQLPGKYSRMVGEFKLEDDETDKIKIEVFEEKAKLYGDEVQLTVAINKKDCEKCKLLAVLSGDLNIKEEATVNGQLYLYKDEEEIEINQEIQDYLFERDENNYQLKLPQQFKILQIGIFGDMLPLLKVHKKDNIYFSSQEPIQLQQRGQAFHIILRREDIEGDEVTINLSQGGSMKYLMVVKLIDEAILYNNQLILSKIQDKGKHNYLFKTIESEQEVGIKLVYFEKGDNSIRIAECQNGCQLSDEDNHNLRFHKNEQKPAVYITPHCQNKDKEGFCIFQLEISSNNKNIYMMTTQNDNNQRILKSDVAYTNYMEQSQEEHLILKYQKSDDDEELVFTVNTHNIVYMISRDSSCYPVRIECAKHFGGVDHLVVLRGDQLKHTQYYITLTAREATIFQFMAKVTKQADINIKVLKEDETYRGVLQLTDGQSKIHYFRVIINFNQVDQNIEVIDEQQPTIEFAIHSSRMQVALTLNQGNQLPSLKSFDLITFNNYLSIQPQDQFYQNSGNYTIGIQNMFETFLDKEIMYTFTYSTSRTVKTLHVGQQFVDLVKGFKSKYFSFYYSQNTTIFYISLQSKKNNKLTLTVQNDIKPDNHSQFAKSQDSSTLKLSESALNSLCLDGLTQQDKDDDKYVMAICQAYIIIENHGNEDILFNLNVWNPQSALELKDGQEYTFNLEYLFQETFLYYKVISSQTDVQLHINSHYGYTKYQIQILDSQDQESNSLYAKEEYKTQHSKSIHSDAFKFCEPDCVLKILLQAQDLQYESDRQINFDDTVYVTITQHYMDLKSGLPIQISVNKLQPRKFIFSEINDISPQSRFKLILHQIYGKGSICLTFNDEDHINLDTCEFNVLGNVLELTQSQIQEKLTALNLTRNPYIMIQIYSLVDNSKFQLSLEISNDMNNHKLIMGIPTRIKLDIQEEVQYQYFNIQNNDDLYFKFIKVQGTSMIQISRCLDNIKKDCEEEIIINEQLLAGQFYNQHIIHKNDKQKYCELCTYIIKIKTIGINVDLLVVVTSQLNFVQLPQNIAFTDFLENQNDYNIYHFSYDTDHQIEVQINQYNGDTQMWIGYNAVLDSTLYHYGPYDIIKQQKLMNTSSSISYYQAIIPPREHLEETNYTPYANGSHTLAGHYNDDDLYIIVKNNQQKPSNYSILVSQSTTGNGQLLQDGIITFAYLSKQTPIITLYHQYRYLKQAQLVIKMTPYGMRLQGLEYFKIEISNETEPTNFTLLTPISSRNDQYTYILPILEGLLTIKIHSLLEIKGNENNTIQYVFNREGDIVPIRIYQFREIYNYGHNYLNRIDLQISIVGKEILMIDEKSSLNDQIYDSQAKYYESYIPHEGKLAVQVYNCLGELKVSITQNYNEFIDGIYNGSKTTIEGQLTDIILPVIPGPVYFKFESIKSVYKFTTQVYKAQDFAPYGQLVMGGDGQINYYFETYDTDIITVKFKPLKCIGCDLNQEMNSLIKYSISWGSNVEYAHVIGLCQYSWYANYHINHTDNFQQVDIGLYALNHTDQILFNIIVDKQSKHPQLFIAIRAQVLVFNNLTIQDYELYYHVAQIGMPNISLYWYKHRFNELIVGVTIFLAVLISLSCVLCKIFRKIKKLKKENLNLQLEKQMEEAKEKVQNQTKYETLQDENNDQSNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W2T2</accession>
    <name>A0A8S1W2T2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0830194</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000083">
        <property type="protein sequence ID" value="CAD8184688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W2T2"/>
    <dbReference type="OrthoDB" id="292656at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8184688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="70" mass="8111" checksum="5C55C2F5FFBAAEDD" modified="2022-10-12" version="1">MQRLLKPIQIQQPVLQRMDNARSDCSADEDLSFIEILRAEAQNPMIKNFSQEDSIVDDTKDLEEFLNSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W5T5</accession>
    <name>A0A8S1W5T5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0830216</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000083">
        <property type="protein sequence ID" value="CAD8184731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W5T5"/>
    <dbReference type="OrthoDB" id="306004at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF274">
        <property type="entry name" value="MYB-LIKE DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="3">
        <location>
            <begin position="65"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="3">
        <location>
            <begin position="128"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="4">
        <location>
            <begin position="157"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="3">
        <location>
            <begin position="191"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="4">
        <location>
            <begin position="195"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="245"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="286"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="245"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8184731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="477" mass="56638" checksum="27F838F120A1833A" modified="2022-10-12" version="1">MQSSENLLIEDFHQNFLNFYAKTKDITNVRFNYMRELYNLGVDTFPDNQEVYTIQQNNKLNLQSQQNKLKKNWTDDDKKVLIWLVGKWTAFNRRDIKSISDDDWNSIASMMPRRDAFKCKQKWLQMLKLPLQQAPWTLPEDDLLRSIIYDYQNLNKGNKWSQIATTLNKISESNIHRNGKQCRERWNNHLNPFINRNPWQLNEDLDLLQLSIQNGKKWALISKKLKIPRSENSVKNRFNCLLRKERSQKAGKKDEEESESEESKISNFPSAEELNHDEIKLIHTIIKKIEWRIQQSENLRENEQKDIVKEEHLDIIKKIKVESNLPLKRDLRHSQSNPNNQDKINLQVKECQLTEVEMSTLQPCLINKEKNQIFFVSPEQLHIYLNRSEISQQSTFQENNLSNQNSIGSYYYDPKLLRSYVMMQSNEYPQIQSGVYQQRSIINQNMLGNFQSLNNYPYPLPMSSYPSQANLMGQLQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W903</accession>
    <name>A0A8S1W903_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0850069</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W903"/>
    <dbReference type="OrthoDB" id="303600at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8185407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="201" mass="23822" checksum="7F243EC8025AA1BE" modified="2022-10-12" version="1">MYNNKFPIELIKKKVQENTISYKFRWNNGTISIEPMIQLTPQLLELVHQYELKQYYEINKKVKLNQTEIPSEPLRDFPSILTKDETKISLLKNEDTSKTIQKAPIKSLQESNYESNTILPQDIIPQIQQSNPKQYLQKTFVKHTHLPRQNKKCIIKSITRNNGDIMFLINDDEIKWVKLEDLKRNSPITLCDYLLSKVRFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WDL4</accession>
    <name>A0A8S1WDL4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0870215</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000087">
        <property type="protein sequence ID" value="CAD8186715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WDL4"/>
    <dbReference type="OrthoDB" id="294251at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.750:FF:000003">
        <property type="entry name" value="GTPase activating protein (Evi5)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000085">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF9">
        <property type="entry name" value="GTPASE ACTIVATING PROTEIN AND CENTROSOME-ASSOCIATED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="101"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8186715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="367" mass="43685" checksum="876F6AA7B3D21E82" modified="2022-10-12" version="1">MKNNTINPSKQNQCIFDLLPEASPTFQTEFKKQQPIKQETQIMNTQDQTDKYGFKSQKELKQKNDPKTFNARIQKWRIMIDIFDKQEKPDKKLLKNRTRKGIPDGFRNLAWPYLAGVPQAKIDSILYLKCGKYDEFLKSQEFKFEQQIRLDVLRTFPDNVNFQDQTVLSESLVNVLKALSVAISDMGYCQGLNFLTAALIMVTNDENAFWILFRLMTKYNQAEKYKNPSYLLREFFILDSLINQYYPTTAKIFKKNNIDLFYFATEWFITLFASTLPIDLFYRVFEIFLLEGEKTLFRCALTIIHFKEQKLIQLQNNFENSLRHLKSLDDFFTLDNDQFINVMLNKFKFSRQLIQQLDEKYKKQKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WFE2</accession>
    <name>A0A8S1WFE2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0900167</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8188033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WFE2"/>
    <dbReference type="OrthoDB" id="10261027at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="96"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8188033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="364" mass="42966" checksum="C722F33FF245CB0E" modified="2022-10-12" version="1">MICHKKYLIFKEPYTIELHSNKIVLQNIAQKITHILQLEQGSVVDWKLDKKDCKVLALGIQIDNKWEYFYMKQQNLTLLKDFLDGKVQYHDIFKLYLIIGFLGKGAFGKVFKCQNNYNGRILACKSLRKSSKYNEKDFINEVQCMQNLKHPNLVQLKEFSIEQNYFYILMEYVEGETLKNLMKNNLLDEKEKMIIVQQLLSLVNYFHSEGYIYRDFKPENILFIENNVNKLKLIDFGLTIKIDEIVSNKYQVCGTLGYIAPEVFQRTYKYDFKSDIFSLGALIYELFSGIYLLQNCDSQELQNMSKKFQINKEILSNIKNQTIQKLLYGMLQEDPDQRLNSREAIDIFKQGYQNCDELLTTNTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WJ65</accession>
    <name>A0A8S1WJ65_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0920090</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000092">
        <property type="protein sequence ID" value="CAD8188785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WJ65"/>
    <dbReference type="OrthoDB" id="303432at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050930">
        <property type="entry name" value="MFS_Vesicular_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506">
        <property type="entry name" value="GH10249P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506:SF26">
        <property type="entry name" value="MFS-TYPE TRANSPORTER SLC18B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="298"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="327"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="415"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="447"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8188785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="480" mass="53828" checksum="D7E73B9E17CBE715" modified="2022-10-12" version="1">MKFLSEFSLLNIGNLICCLGLSVIAPFYPTYAEKFDVSGSLLGLIFAINPIGGILSSLIVGKILNKNNQTNLLSLGMLIQSIGMFCYPFLTFTTNRTLFIIISLLGRFISGFGAQTFVTPLYAIITQKYADSINQKMAITEFFSSFGYLCGPVIGSTLYSIGGFSFPFIVFGCFSVIIAIILKLNFEKQEKRAINKVNESVISDIYDIDCSSKSLFIDEENDVSIGYFDLIKYFPVSSSLFVILALCITFTFYYPTYAIFFEENFNIPPQHVGFYMSAVSLAYSIGALTISRISVNKSIAIFTGMVFVSISQFFMGPDPIMNISPRILITVIVKLINIYKLILFKRMKIQFNYYNLSIQINNSAQSIFGFFSAPPYIMALPKITEELQNRFQKSDHDKCIIELNINCYNSLASGLFNAVISTGDFLGPVFSGVMAEFFSFQRSCSFLGLYLVATTLLFLPNLFISKKIKKVQIKSYKEYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WKK8</accession>
    <name>A0A8S1WKK8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Leucine Rich Repeat family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0970044</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000097">
        <property type="protein sequence ID" value="CAD8190644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WKK8"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052394">
        <property type="entry name" value="LRR-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8190644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1090" mass="127874" checksum="55C3EF5EA95F0454" modified="2022-10-12" version="1">MISHDDTNNKQLSQPIETFRRLMLPHKSDVGLGQQSWTKHPNTTKNLQRKLYITNKPEVNINQENQNTSSSFEKNIADELLKHEVCDWQFFMQKDQEIIQQINTSDEFPQEPTYKAKTLESTTRNSQLQKGNERYLYFQRMKQGNKLLPNLSTQDFLSSVRNSNKLNEFAKVYKQQPQNLYLSLNLSQPQRVPKTFGLIQNTQQLVSVNTNRCLRSREDCRAFSFAMQTQQSKSITSLQMNHNIFDPLQLRDMLLSFPTTLKDLELIDCKLHYQHMDVLMSYINKNQISKINLEKNNIRDQGCKIIVKYLMNNNTLQHLNLNNNQITESACMSLSNLLKQTQRLLELYLGYNHLNSSAGNTIWKAMYKNTSIKILDLSHNNIASLDCASSIAKAIARPYNELLHVDLSYNKFTYLQAQVIADALIKNETIYGFHFEGNQTELVVNQNGFLINNIQQKKILNDKLTLLYKQKQYFKLLDQPKKDIQTITNQYEEFVMPYSRQRKIRSTKLNVQKVNQINKLDTCWICEGWQEIKFEWTAYKSGSLYNEPIFIHFDFEDYKPLLMTHLNHEFFLVKMCPPNQNIHYFFTNPILGVQQPALDQPIIQLEIPLSSIPFLYNDEILVDGNIIESLNVLKTFNKQQLFDKYMPLVQCKPREPLAKFDFSPYLNIKKHDWSVESSIFRNFQPDTPQLIDQCFEFDFQNSKVTRLVKENEVGEVKECLRDLYRNLFHVYKYHASGSINTPIPCIMIQDFIDFLVQTSLMDGMKTNDIDISFTSTSAAKDVAFPQAYEKGIVRCQLLEIMIRLCNDKYIRSGIYTSMVDSINAIKQQCQEYFSKFDQSQQWRKTRLWSQKCDILLNDRLAMLKSLYKYCCKLSKKPQQFKFEYVSFSDFKDFISECKLICDELSERECYLVYLQSMVTQKDELYSSKHYQMNFHEFIEACSRLAEKLSIIRGDKPIDLDDRRAKDLHTKLDGFLLYVYLNIGNQMKTILSQNDPDIKGVDKCMINDFKSLKQKLEDSFTDGDEPPYDPKVELPHLSSQITNLLGNTFGGKKQTLRQQIKQQKKEEQKFALINFVQYFKSIQQINKDQDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WMK5</accession>
    <name>A0A8S1WMK5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Calcium-dependent protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0950007</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000095">
        <property type="protein sequence ID" value="CAD8189851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WMK5"/>
    <dbReference type="OrthoDB" id="288147at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="23"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="390"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="428"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8189851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="461" mass="54807" checksum="98A9A6A25BADACA2" modified="2022-10-12" version="1">MLNLSNLWFAWSKQSKLKDRYEIEGRKSLGPSKYGLVIKYREKKKDGQIRTVRVVPKTKVINQSRFKQEVNQLRYLNHENITRLIELFEDDKNLYFVLEYCDGGSLFEKILLRYHLKQSEARYLFQQIINCLNIAHRSAICQRDLRPESFHFINKDINNLDIKLIDLGFQLIYVDDYIKKSNNQIIDTSRQGKLYFTAPEIFNGKLTEKSEIWASGVILHVLLTGELPFDGKDENEIYQNIQDNTIQLKTKGIVSDLIEKILQPQENRFQIFQIQKHKWMTQQISEEEEILDINFSKLKSLTQSNFLQKIMRGYVADQVKQGFAENLRIVFDQLDITGSGFIKIDSIQKSLEKHQEFEEILKLLKYVQTEDGTINYLDFMNQILEKRVFVDEERIYKTFKMFDLNNKGKISNENLWEILSKLENYKYITKEYCSLLISEVDRDQDGEIEYLEFIDMFFKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WN12</accession>
    <name>A0A8S1WN12_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Synaptobrevin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0940112</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WN12"/>
    <dbReference type="OrthoDB" id="248747at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd14824">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15843">
        <property type="entry name" value="R-SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051097">
        <property type="entry name" value="Synaptobrevin-like_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136">
        <property type="entry name" value="SNARE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136:SF168">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13774">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01270">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Longin" evidence="5">
        <location>
            <begin position="6"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="6">
        <location>
            <begin position="125"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8189609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="212" mass="24791" checksum="4914FAD4B0A1D908" modified="2022-10-12" version="1">MILYTLISKGNLVLSEYTELEGDFTEMARIVLKSVKPNQQKQIFSKVYDGKKQNEYVFCITCISNLRFLCMIHNSYNKEKAFAFLDAILQEYQNCSKNSEFNNGQFSSQLCRMMKAFNQLDQKDALTNLEDTVEDLNESAFLNLRKMIEREVRLDVLVKKTEELTKNADIIFKFSRNVHRASRGNEFRFGGKILILSFVIFAILSIYIYYQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WNU1</accession>
    <name>A0A8S1WNU1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Polyadenylate-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0950149</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000095">
        <property type="protein sequence ID" value="CAD8190135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WNU1"/>
    <dbReference type="OrthoDB" id="5970at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001755">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001964">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001288">
        <property type="entry name" value="Protein CBG15138"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1900.10:FF:000012">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002004">
        <property type="entry name" value="PABP_HYD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48033">
        <property type="entry name" value="RNA-BINDING (RRM/RBD/RNP MOTIFS) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48033:SF10">
        <property type="entry name" value="RNA-BINDING PROTEIN SQUID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00658">
        <property type="entry name" value="MLLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51309">
        <property type="entry name" value="PABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="9"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="87"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="166"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="PABC" evidence="6">
        <location>
            <begin position="436"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="350"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="367"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="419"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8190135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="516" mass="59517" checksum="196BC299CB2F913F" modified="2022-10-12" version="1">MIQSIPHDRCVYVTQYKPETTLEQLKQAFSEFGKIELLQQNAKSNGVFIVFDSHDAVNKATSKTVTIGGKNLDVNEYLDMDKLDEEANIFIDGISPTTTQLQLKEKLQKYGKILNIKVQMSDKAGVAYIQFEERKSAEQCLNDQKNLGMHITRAIKKGKQQQIKRDQLFISNIKSQLSLDQIKPQLDNYFSEYGKIELIQVNVNPKNQTYFAFVRFENSEDAYKAVKTTKPFQNQIINIQWAVNSNDNIEANLYTKNLKPTVTEQQLREAYESIITLDDLQLLPPNKSNKLSAYIYLKSAEDGKKVIDLAFKTKKIFDLYDKLTVQISPMLSPAEIIQFKKIQSKPTYQPYANSMPFPPYQQPLQGYPNQQNPPYGAYPQQQPPYYAGGYPPQQPPYQQGGYYQQQGNYNQHRPKKHPYPNQYQNQQRQHQPRIPDLQYLQNLLAQGNEKKVTDTIGILLFPLVNQQVEQKLAPQVTGILLDFENYGLEDQLKMLQNAPYLSEQINQAVDLIRKSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WRT8</accession>
    <name>A0A8S1WRT8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1000060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000100">
        <property type="protein sequence ID" value="CAD8191882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WRT8"/>
    <dbReference type="OrthoDB" id="295628at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="126"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="340"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8191882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="583" mass="68287" checksum="96AAA3D8A65B2031" modified="2022-10-12" version="1">MSESPHQLKAKIDFLHQENQQLRNNIEDLQTVIKLNKQSMRSMIDQVVTIPRHNQQTSIHENENTVKALKLVITEQQKEVDHLMSSLDKVQKERDFAQGKALISEEITYAAEKSEKKLIAELESTIYELNHTISQQAQKISQLEKQVDQLDDTTGVVVRYRDVIHPNSATILLHEEMETLHQLIVRLQLQVNYLKSIQLKLVTLINKVITNLQMKNQGEFIDLDEDYEKAQHEFLEILAQIQQSRLPTVELPVRINSIQSPMNTDMMHSPILSPFPNKAPQVQKKNYQTLNQTFDGAIGINDSYQQNGRQEDLQKNISRVPKLNLARAFQIQQINAKRSTQQIKLTEDQLQNRVRKLELELEETKKNLSREMILCKHYEIENDQLKRYIKQIEANNDILVKSNLNLNQKWEKIQKSFMYYKTFFVEHKDQFAQLQILVQSEKEKAQSQEDQNEFGFNSVRVSHFRKGQTQRKKTLLDQTITSYLPMYQESLMEIENQLGRVNQSVTYNQNNRINSSCAIENNGESPTIYFDKNDSDNENTEWRNYLKREWTILATQVHSAKPKNLVQQIKQSQPIQKLQNIKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WWE2</accession>
    <name>A0A8S1WWE2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1060147</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WWE2"/>
    <dbReference type="OrthoDB" id="10056939at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="5">
        <location>
            <begin position="115"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="4">
        <location>
            <begin position="117"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8194344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="213" mass="25289" checksum="EC41EA401E1C5BF6" modified="2022-10-12" version="1">MSQSLTIHQELRKQFVLQQVSNLESQLTKWVSHNCPNEDILQSHSDEAHSQSQTHINVIHFQGLLMYLKSLSENPSQLEFHTLKSLQQYFDYMSQILQNELKLSYQRQTMKISDKTIEKKSQKFSKKSNLILKNWLNKHYFYPYPSKEQVEQLAEKCNLTQKQIQIWFINARGRIGNKMYEEKKFKNIVKSKYLSLNSNKASKEKLEINQIDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WXM6</accession>
    <name>A0A8S1WXM6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Xaa-Pro dipeptidase</fullName>
            <ecNumber evidence="8">3.4.13.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Imidodipeptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Peptidase D</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Proline dipeptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPENT_87.1.T1040045</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>Xaa-L-Pro dipeptide + H2O = an L-alpha-amino acid + L-proline</text>
            <dbReference type="Rhea" id="RHEA:76407"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:59869"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:195196"/>
            <dbReference type="EC" id="3.4.13.9"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the peptidase M24B family. Eukaryotic-type prolidase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.13.9" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WXM6"/>
    <dbReference type="OrthoDB" id="10261878at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102009">
        <property type="term" value="F:proline dipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01087">
        <property type="entry name" value="Prolidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007865">
        <property type="entry name" value="Aminopep_P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052433">
        <property type="entry name" value="X-Pro_dipept-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48480:SF2">
        <property type="entry name" value="PEPTIDASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05195">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01011">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0224">Dipeptidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminopeptidase P N-terminal" evidence="14">
        <location>
            <begin position="14"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8193357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="480" mass="56374" checksum="19C5480985C513BB" modified="2022-10-12" version="1">MNTDPIPPPREYVIPEDFHQNIRTRFLNFFRYTVGDKARNSVIMLKGAYKINKHDEDQQYRVEQESNFHYLFGVDFLNCYAIIDVDNGKSIVFVPQYDSNYKMWNVVLNNDEIKQKFKLDEVLYNEEIESWLNNRKPSLIYYFYGIDDYSHHSLPIPDQPFLQNYNSDYDELYYILTECRVRKTPQEQDIMRYICKISSEAHELVIKNIRKGNKEYQMEALYQYHTFINHGCRFTPYECICASGTNGSVLHYEENSKTINERELILNDMGGKFYGYCSDITVTFPSDGRFTQKQAIIYNAVLDTQRQVHNSLKVGVNWGDMQLLAERTITKHLYNAGLIKGSMEDLIKNSICRLFFPHGLGHMLGLRTHDVGGYNKGTPPKIPELSQLQFRRDLDVGMIFTNEPGIYFIDFILQEAYKDPNKMKYLNKERIQEFMHVGGVRLEDDILLTANGPEILNDVPRTIKQVEACWRGEDWRQIND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WXX0</accession>
    <name>A0A8S1WXX0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0970076</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000097">
        <property type="protein sequence ID" value="CAD8190706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WXX0"/>
    <dbReference type="OrthoDB" id="299647at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8190706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="340" mass="40055" checksum="13835A435CE8818B" modified="2022-10-12" version="1">MKNYKKVGISNTKHLNNEIGYYKECVRFLPSERVDDYCTSDAINYDRLREAGMKRNKALQFCTANHCRIDSTQVKHGPPGLKIFKTKIDKFKQLNGWQMVGRLNYVETMQQEYSDDSKWQIQVQNKEESKKLEQLKQFYSDLDIKYSFNDLNLDQTITKLRSLSTEQNYKSRVPQHQIKDSKNNIMYYEMAEYILQLKKPQNTLISDLCAFDDSLPLARQEYYEIQPKRIENAAPLYSPQNCEKRSKLLKELTRAYTGNVTITEPSEELKKVKLLIRLIIITVNMQKVINKKMESLKVQMKMNNQSQGKQNKDVVQRLKEQVDNLLKKKEQIKTYSSTIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WXZ5</accession>
    <name>A0A8S1WXZ5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T1040115</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WXZ5"/>
    <dbReference type="OrthoDB" id="1413014at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000195">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FTSH 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076:SF97">
        <property type="entry name" value="ATP-DEPENDENT ZINC METALLOPROTEASE YME1L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076">
        <property type="entry name" value="METALLOPROTEASE M41 FTSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="13">
        <location>
            <begin position="250"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8193497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="648" mass="73651" checksum="1B5ACC7D92B44867" modified="2022-10-12" version="1">MKGPFLWGFVYKSLFNFRKPSFKFLHSPFSLNKDDLIKKSVNIPDHKFDFQPFIQPDASFLVNQQNIQNLRLLQKEFASDPQNPEKAFKYIRQLNRMQLYDEALRIFKQTDDFRENATQKQHQQLQEQYGIAVTNLKSNQYGDKRRSLLLPGMIQLLITAAILYQMMYFFQPGQSKSKNEKNQKDVPEQGAGRIDRFYNILRNNQTIQEERNIPTRFNDVLGIDEFKEELEEIVEFLKHPKKYTDSGAKLPKGILLVGPPGTGKTLLARALAGEAGCAFFYKSGSEFDEMFVGVGASRVRDIFKAARQKAPSIIFIDEIDSIGGRRRAQDPGYSRDTINQILTEMDGFKQSESVIVIGATNFEQVLDPALKRPGRFDKMIHVPLPDVKGREQIFSYYLQKIKYDIQKVLPSNLARQTSGFSGADIQNMVNVAILNAIKYDRQIATTDDFEFAIDRIAMGVGRKNMHVSDKEKLMTAYHEGGHALTSLLTEGAMPLHKVTILPRGGALGFTSMLPEKDQLNYTRKGIIASIDVAMGGRAAEDLFLGKDDITSGCSNDLAKATDLAYMFVKQLGMDDKISLISIQSDRVKTSDQFDYMVDMEVKKILEESYNRVKNLLKVNESKLKDLATELVKRETLSAEEIKKLLQIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WYQ6</accession>
    <name>A0A8S1WYQ6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0980048</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000098">
        <property type="protein sequence ID" value="CAD8191036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WYQ6"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="385"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="767"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="822"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8191036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="896" mass="104837" checksum="937D346E2248F111" modified="2022-10-12" version="1">MKIGIQQRFIICNLIASILFLILGLTQLINTLDSGLFIIVDLIFSLTFLLLDYKQKFICLKGDYYLALTQLVMQIVQIEMTIITQQKGIFIFIILKLLALTKVLRVQLLQKLIYFIVLIYSIIRFNEYQYPISYLGLIFTPILMLQEQSDENDLLMLLKDILPLPLLVIDKSSRKPMYHTKALENEYKYNTSHSEEFIECLNHFFSDNKIKLQMLFDNSKDFLYNPQTRFRSIFTEIMDNCQYLAQSPLFQKFKDLHEPNNDNDEQMEEQKKQQFDQVHDFMIDTPKYQQFNRIPIQLSEETPKNQKSKIMEEFLIQSQSQRNICVNELKKKRLKLFFNHCFWNKNEAFLLIFQNKEIEKSLTQFQQQLIEQKQICENKDMILATVFHDFKTPINGIVAILETLESKQDLNPQEKYYLNIIRKNVYLMLYMIYDIQDYARIEKNQLRLCISDFYINELIDEVIETISISAEQKGVEIKTYYDIPFYQVHSDPNRIKQIIMNILSNSLKFTEKGSITITVQSLNTDKSQNIKRSNSSKNISFLNANQSISQIRKSLQGKHPTMSFNKLVYTISIEDTGCGIADSVKPQLFNLFATFSSQKIENKNGTGIGLMVCKKLVSLLGPLDTIDLQSELNVGTKMTFQIYAKLQDNTYRSSNYVSCFKQENSSQHLSNFQNDESPNAKDKLYQQSLFVRSSYLRLYTKPIDKAETEMYEPSLEDADQTKSKNILQFQSLSQKTQLQKCLQQKDPLEVQDPKFIIQQLLQSQKFGILIVDDQAFNLLAFKILLQNLIPQVDVIEAYNGQQAIIKLQEQQKSLNIKYVFMDLQMPILNGWQAAEKIRKMIDNKEIDNIKLIALSGFDDESQQEKCEKSGFDAFLSKPIRIEMISEVFYQLEKNFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X0Q0</accession>
    <name>A0A8S1X0Q0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1110014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000111">
        <property type="protein sequence ID" value="CAD8195888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X0Q0"/>
    <dbReference type="OrthoDB" id="311206at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="437"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="525"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8195888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="742" mass="87043" checksum="AB1E8DD683FC3789" modified="2022-10-12" version="1">MDNLLENKCSKHDQEFLAVRIVQDNKDQNKYLCPQCLIELINPKELILISEAKILIQDKKSQISKNMEEQCKQKINLATNLKNSIQELRGPLEQIFTKINEEIDQFKNQTEKKIQESEIITPENLDTDLVFLTDEQINLTQSKTYENDLQLIKSFLNQFQFCEISSLSKQKIIEIISKCYDISKLQIETEDERNQKTKQTPSLKFLCQSHDQQIIMIQLDENSKFQYSCVQCISEKQGKYITLRQLQQSLEAYNQYSEQSLKYCQQLREQHTNKIIKICKQLCGKYIQTFNLQIQQLEVNLKSFEKIIQANLQFKDQNIYQFEQEQLLQKLEPIYIDQNPNHYFKIIEKQLELDKQLYGKFEQQMQELMQFEMSSSNLIKLEQLDHQLILKKIENQIGQQKSNQQNIQNDQEVVNLENEINSFLSKKLKQCVLNEQFQQLFQNRDKLQNDQQQLIQNIVAIQDKLKEQQKNFAKEQGISQQYYENKIIYLEKIKTQLNSTSFSDQIQKETQIQTNETIASFDNDIKKYKQKIAQNLQNIAQLQEKNQALQQDTLQSILQNPYQPQFNNSYKLAAYLVVNHKLVQSPYGEFGIADQGEYGLAVMQPPLPKDNISTFVFKINKTNSWAGVGICHLQTAQGFSYDMNKNYTQKNHNAYLACAGGYRISSYQGYIVLILYITRKYGVNNFTYGEHSIIHCLYNPTNCQLILYHYNDGKTYQMDIADNNLDMSPCVILYGAAEIELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X386</accession>
    <name>A0A8S1X386_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphonopyruvate decarboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1090095</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000109">
        <property type="protein sequence ID" value="CAD8195391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X386"/>
    <dbReference type="OrthoDB" id="16262at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033980">
        <property type="term" value="F:phosphonopyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032923">
        <property type="term" value="P:organic phosphonate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03371">
        <property type="entry name" value="TPP_PpyrDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07035">
        <property type="entry name" value="TPP_PYR_POX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000100">
        <property type="entry name" value="Putative phosphonopyruvate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000101">
        <property type="entry name" value="Putative phosphonopyruvate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017684">
        <property type="entry name" value="Phosphono-pyrv_decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051818">
        <property type="entry name" value="TPP_dependent_decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03297">
        <property type="entry name" value="Ppyr-DeCO2ase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42818:SF1">
        <property type="entry name" value="SULFOPYRUVATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42818">
        <property type="entry name" value="SULFOPYRUVATE DECARBOXYLASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="5">
        <location>
            <begin position="37"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="4">
        <location>
            <begin position="256"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8195391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="404" mass="45469" checksum="A1CBEB1097E4EBE0" modified="2022-10-12" version="1">MFCRQLTRRFFNAKSREPIEPPYLELQRDFLEPAEFYQELSKHGVTFYAGVPDSLLKDFCAYVQDNALPGQHVITVNEGTAVSMAAGYYLATKKIPCVYLQNSGLGNIVNPYMSLAHPKVYGIPMLYLIGWRGEPGKKDEPQHLVQGKRMNGILTEMGMQYDVLPDYIEGAAEALDTAFYSMKTRNGPYALLVKRQCFTNYKLKNVAKNNYQLTREEALTEVIQNTRPLDVCVSTTGFLSRELFELRQRLNQNGEQDFLTVGSMGHASSIALGIAQVKKSRQVFCLDGDGAMMMHMGALAQIGNIGPINYKHILFNNQAHDSVGAQPTSNPLVNFTQIAIGCGYKDAFQVQTKEEITKGMERLNRNDGPILMEILIKPGVRKDLGRPTTTTFENKEKFMHFLTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X4U7</accession>
    <name>A0A8S1X4U7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1140064</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000114">
        <property type="protein sequence ID" value="CAD8197003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X4U7"/>
    <dbReference type="OrthoDB" id="294165at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="51"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="184"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8197003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="317" mass="37927" checksum="1844CD75F9A4C02B" modified="2022-10-12" version="1">MQKRDKSPIIHVNLMKENIHTKPHSLNKNNISNKASLNLVKVLATRRSDMNSLWEPPEDSKQHSNRFDNYFQRHSTRRSNVQTSRERERDFSDSPQQSPSHHQCIRHINKTQQRKRIVQFLCCLTKCDTHLLCRECKYKIFQCKHDQIVKLTEFIEQPILIFLKKDNSQSELIGNLILQQMQLFNKVKYQAEQSKQQLQEYLENMQDQLKMKCQVIQTEFNEKVDYQLKQIRNDIDQLVQTSLVYKDDFDNRLNQNDDFIESVKLFKQQSEDPYEQVMNQVNQLQQNLNKIPQLNSIVLELKTDITIKDFNYNKLII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X7A9</accession>
    <name>A0A8S1X7A9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SF-assemblin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1140162</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SF-assemblin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000114">
        <property type="protein sequence ID" value="CAD8197191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X7A9"/>
    <dbReference type="OrthoDB" id="298686at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038835">
        <property type="entry name" value="Giardin_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008374">
        <property type="entry name" value="SF_assemblin/giardin_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37027:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_148, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37027">
        <property type="entry name" value="KDE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06705">
        <property type="entry name" value="SF-assemblin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="34"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="218"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8197191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="256" mass="29567" checksum="73EFD6BE70E05DA1" modified="2022-10-12" version="1">MSRVKQSAYVQPSPSSGRIQKISEKLSTIQIGVENERFQKLEQAEQRIQQAEDAFNEFQEQIFTKLNGLRDQLGKLQKQVEDDRLAKEQANEAKNRDVQALTKKFENAIENEQQTKKEGEAKVLRLTEDKTALLRTEVQKEIAQRVDAIEGIHQGLQNDLPKIQEAIREEANERDEADQNVMKSITDELVKLSNLINVEKRNRDESEQSIFEMLKDIVNRVKVELDQEKRTREQSEEHLLSLLEDTCNKLSIAANL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X856</accession>
    <name>A0A8S1X856_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1110013</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000111">
        <property type="protein sequence ID" value="CAD8195886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X856"/>
    <dbReference type="OrthoDB" id="2104158at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33487">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33487:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="520"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="383"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="599"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8195886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1188" mass="139491" checksum="07E63F2BACE1AE3A" modified="2022-10-12" version="1">MAHKKQQGQAKPGVKFQDQPASIQQVENKPQVIYKNVTEQDQALKQFNQATVLIKFRKDTAQSLKSLLTLLRDCMQQISSLQSKACAYRIIDLGLQKVQELRELEEMDPTLQDYMDKILKQNTRLLRILELKFKVQAGVLTIDQWKKKIEEFSQDKQARCLVIIESLRFSPPNLSNTVKQTHVGNQLKEQLVIRIHNQQKKQLTREFGLKYGLQNENIFNYAVAVLVYNIHSGDIIENWEINELRCKFLIKVESILSNTLDKLNQEEQIEQRILTKPKEEILADPKMEDYYHLQGISKQVGVINNQEALTYLQKREERMYMMARSRNLMAKVNVSMGQITRALHIFKYAIEKLQSYTLELSTNENGEEVENLPWDQLKPQGVVEDKKGAKKAPEKKGKEGKESKDKPIADEVPAVKTDAQIKLEETILQIAFIKCLLLDQIKTELTLLLYRHNRHDEALEYMETLLNDCKQLNDNYHQRLIIEYQARIQFKKGKQVESIKKFKEAIEIGQKSFNQDPQMIVLYGDLGKIYYEKNELQEAQQQFYDAYQMAEQLMEMINYGYGVIPNWNQKCVQEKIQICQDLCVPLEIEQEILKKTDKQIKTTKKDQKKKDDGKVVRKGADKQTKKYNIDKPQMPIKGLNQQSNFNFIQSTKLTYTTVDIDTINNTQYQYNCYLKNIELWQRASLRYVDIMITLNQANENSNISIDSQESSVVSDSESDKSSIKDIFDINVINEIVNKIHTSISKSINVPISFRLELYYLQSKIYKMRFVQLLLNLQQKYFMKYLSGKNKKYFDFYEKRPHRDLVRNKYFLLIPPFYQLLKEQGLQYLMQAKESLLKAISVIRGEAVLFEYNRKREDILVAMAEICLFIREYRVRQGYRYVKVDYLQSLINTKNTEQNKIDLEQQLISMERSDRLEQLDLELEAQSYLKYAIELVKARQEQQENFAVVSLTPLPDVKKLALEIYTEIIEQDYQYKKNTILFDVSSMDILQFIQKVGREAKVMTFSGSYLQRMISKTHRFFKLHMQQYQKCLITLLEVAPKNHVNSVFIDEGTIVTKLLYNPVAHEFKVLYILGGINKEKIITSLSKEEKDKLIISDEKNVVYGSIYITDTNISILLQDALNLQSKMKESDQQSQKMKERDYKHHKKAFYKLIEQLGLYFFQKPSIIIIQILVQFICKMAKLLKTQKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X8Q4</accession>
    <name>A0A8S1X8Q4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1150076</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000115">
        <property type="protein sequence ID" value="CAD8197378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X8Q4"/>
    <dbReference type="OrthoDB" id="293541at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8197378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="287" mass="34517" checksum="96B53B89C691C18B" modified="2022-10-12" version="1">MHQFLPNQLIFDSYSSINYWNAYQNLQNSYYNNLYAYQQMAYSQPFPQYPQKLQQNFEQVHYYEDPISTQGTRRSIVEYQDVIPQVTNQTLERYIMMIVNEDETFKNILTQLKNSNQIALVKLLEMLAQKQKCQQKSREELVKFCLRKALKFIFQRIQEKYDKTKTNLKSAQKIFISMAEKETQTTIMLPFRKNSKNKTMNSDFLKQIFSSKTFVTYYKEFLSCLEQEIQKDSKKKIASLCNKIMNHIQDDKRFEFDVKRLPWSYSNIEKVKQAAQEMLLYSNFEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XAA3</accession>
    <name>A0A8S1XAA3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1160115</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000116">
        <property type="protein sequence ID" value="CAD8197792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XAA3"/>
    <dbReference type="OrthoDB" id="302049at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8197792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="226" mass="26380" checksum="A5207BEA4F41A6E1" modified="2022-10-12" version="1">MNRNGLEKICNQATNFWKQQFIASSKQDQDISTNAGSSILEEQSIFEISAKKTEKYLFPDIERLNLNKEKCEQEFTKKQEENYEAEASCSQCSECLSKRRDKKKSKIRSSKSTKKRFTISEEAFIMEQSRNGISFTDIAQQIPGSTVNQVKHHLLKKLKICSKNKDFQGDSIAEIILKIQQLQQNENLDNDSKIQELRQHISLIENHLHQTKQMILQKYMILFQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XGA2</accession>
    <name>A0A8S1XGA2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1220174</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000122">
        <property type="protein sequence ID" value="CAD8199839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XGA2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8199839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="83" mass="10242" checksum="203A53498A3E87A0" modified="2022-10-12" version="1">MYLKTYQKGILSKLLYNDFQIIIYIDTILIQFSSLYSFLNQQCIQNFYFQQNQLSFLYLLYRIIVLTGKFYEQNNYYPNKLIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XH55</accession>
    <name>A0A8S1XH55_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1250077</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000125">
        <property type="protein sequence ID" value="CAD8200680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XH55"/>
    <dbReference type="OrthoDB" id="293905at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8200680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="250" mass="29746" checksum="72C6D3878D384B83" modified="2022-10-12" version="1">MKISDQLKSSLVQCVPEQNQSLYDILSSQQGVDQFQYLVKGFQEKSSEDYMKTVPTPINRQVRSSQSKSQFQQFPIQKSIFEDPGLVQLSYFQFANVMDEFFLEINETQIIEWFQLLQINEKVQIRDFYIIVLMFLSRDSKQQFDYLYQYGEYLFECLKQTNNKILGLTLKIFVKLCGMQARQSQKLFAELKMKQNEEYTFEDFEVFCFAFMNPESLQGQGCQQKFIKQTHQNKQDFNLQQIIKPKIDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XJT8</accession>
    <name>A0A8S1XJT8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1260161</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000126">
        <property type="protein sequence ID" value="CAD8201127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XJT8"/>
    <dbReference type="OrthoDB" id="71437at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF8">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="321"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="416"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="450"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8201127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="527" mass="61633" checksum="EC9B1D56CE0599BC" modified="2022-10-12" version="1">MQQLRHSIECTQIDHDHQQVQYVCIDPSCTQIEKGMCEVCRYGNHLQHSCIEVKQLDELVIKAYEQEKEQNNKIYKTSCQRIKLSIQLLQQKLLQIMQELKDICKMQREESLLELLVETYVKNEKLNLEEYNAVRDLVIGNLDCFFGQYFWKKEIYTNNFKSIQQYSDNLLKQTILFTEDISLFQQYGIQLCNVQIQEFKEYFKQQNSKYQDILNYNEILKKEQFNKIEPIEQFYKKQLQKISSVDFSNKLDLLAIGGSDKYVLIIDFKSANCQQELLISTQQVYSVRFSPDDKYLIAGGSKPFEIYCWIPNNWSLPPIILRGHTNYVYRLIFSEDSRFLLSSSADKTAILWNIDKKLQQQIYKGHTSNVYGCAISIKSNRIATIGGDEFIIVYDQLSANILHQWKGHQCEYGGSSINYLQNGQLMISSGYDGLIKLWNSNNYELIREFIGHSKNWVWSISISQNQELFGSICNDYTIKIWDIKQNKPLLTLQNENNGAPGSEIILKQNQFVIATSTQSIKIWKLSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XJU1</accession>
    <name>A0A8S1XJU1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1250114</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000125">
        <property type="protein sequence ID" value="CAD8200752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XJU1"/>
    <dbReference type="OrthoDB" id="302368at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8200752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="69" mass="8278" checksum="5E99BF90F914C82B" modified="2022-10-12" version="1">MKQLEYKAYEVYSGNLLIDYWSNKQNEHSYPKLEMKKINKKMSKLQIKGLKRKNKEDDQIIDKIVALCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XKQ9</accession>
    <name>A0A8S1XKQ9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1280008</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000128">
        <property type="protein sequence ID" value="CAD8201474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XKQ9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8201474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="77" mass="9355" checksum="49DD4A1150F8774B" modified="2022-10-12" version="1">MPQKKIKSNRIYKISHKNQIKLYDISTNLLFRYKEENNVYGKDKLNFLTLQFRTYLYIKPQNFSIHFIQILNITRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XKU3</accession>
    <name>A0A8S1XKU3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1290001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000129">
        <property type="protein sequence ID" value="CAD8201741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10043504at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="24"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="140"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
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            <begin position="174"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="208"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="242"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
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            <begin position="276"/>
            <end position="309"/>
        </location>
    </feature>
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        <location>
            <begin position="310"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="665"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
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            <begin position="699"/>
            <end position="732"/>
        </location>
    </feature>
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        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
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            <begin position="767"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="801"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
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            <begin position="869"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
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            <begin position="937"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="971"/>
            <end position="1004"/>
        </location>
    </feature>
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        <location>
            <begin position="1005"/>
            <end position="1038"/>
        </location>
    </feature>
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            <begin position="1039"/>
            <end position="1072"/>
        </location>
    </feature>
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        <location>
            <begin position="1107"/>
            <end position="1140"/>
        </location>
    </feature>
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            <begin position="1175"/>
            <end position="1208"/>
        </location>
    </feature>
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            <begin position="1209"/>
            <end position="1242"/>
        </location>
    </feature>
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        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8201741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1278" mass="150158" checksum="7244FD8AE5447B55" modified="2022-10-12" version="1">MIQNDENDSKNEIFINEFKLCQASINNFRQLVSLIKLQILINQQFLLRLMKGDIIQQCIVSQKRNHKIKIRKCIEKQLDNKRNNMNRFEEALEYYNSAIQKNLENGDYYHSKAIALFKMNRFVEALKFFDLAILKNPENAKYYNDKAIALIKMNRFEESLEYYDLAIQKNPENAQFYYHKANALHSMYRFEDALVYYDLAIQKNPENAKYYNDKAIALIKMNRFEESLEYYDLAIQKNPENAQFLSNKANALQEMKRFEEALQYYDSAIQKNPENAQFYSNKANALYEMKRFEEALLYYDSAIQKNPEDADYYHNKAFALYQMKRFEEALEQYAQAIQNNPEDANYFHNTALALQKMNRFEEALLYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFLSNKANALQEMKRFEEALQYYDSAIQKNPENAQFYSNKANALYEMKRFEEALLYYDSAIQKNPEDADYYHNKAFALYQMKRFEEALEQYAQAIQNNPEDANYFHNTALALQKMNRFEEALLYYDSAIQKNPENAQFYSNKANALCQMYRFEEALDYYVSAIQKNPDNADYFHNKAFVLCQMYRFEEALLYYDLAIQKNPENADYYFKKANVLQKMNRFEEALEYYDSAIQKNPEDGDYFHNKALTLCQMYRFEEALLYYDLAIQKNPENANYYFKKANLLYRMNRFQEALDYYNQAIQKNPENSDYFYGKANALQELKRFEEALLYYDSAIQKNPEDADYYHNKAFALQKMNRFEEALEYYDSAIQKNPEDADYFSHKANALYKMKRFEEALLYYDSAIQKNPEDGDYFHNKAFVLCQMYRFEEALLYYDLAIQKNPENADYYFKKANVLQKMNRFEEALEYYDSAIQKNPEDGDYFHNKAFALQKMNRFEEALLYSDLAIQKNPEKADYYNGKAIALIKMNRFEEALEYYDLATQKNPEKADYYIGKALALENMNRFEEALEQYDLVIQKSPLNQECLNNTELSEKSFCEIQQPLLFQDFNFESIQPSKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XL76</accession>
    <name>A0A8S1XL76_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1290097</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000129">
        <property type="protein sequence ID" value="CAD8201925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XL76"/>
    <dbReference type="OrthoDB" id="302095at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8201925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="109" mass="12542" checksum="E8409674F235A548" modified="2022-10-12" version="1">MNQTYLRSKRQYIKSSLQTRKLLAELVLIQGLKIKNAAKRLQIKYATAKSIILYYKQNVIKKQQNCKPAKRCSYASIKSAISYTIVSKLAGQDVNSRCIHFLNMEVSKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XQT5</accession>
    <name>A0A8S1XQT5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1330187</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000133">
        <property type="protein sequence ID" value="CAD8203413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XQT5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8203413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="17071" checksum="A8DC74B7DDA42E7C" modified="2022-10-12" version="1">MIGHTIFLHNYRFIDSILVFSIEYRIKLINVVVLVHTMILHNIMNLLYIHQIQKLLNYTQILITIYFIYFQQLKNFWSLHLLMISVILRDQESKRIIKYLNLNITYLAKYMNKENEINVLLKNFLFQATNASLKLRNVTFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XR61</accession>
    <name>A0A8S1XR61_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1350132</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000135">
        <property type="protein sequence ID" value="CAD8203986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XR61"/>
    <dbReference type="OrthoDB" id="5980302at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="63"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="108"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8203986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="331" mass="39028" checksum="99C5E70EB1407DFB" modified="2022-10-12" version="1">MSKQKQSNSQIYNFEPIGCQIKRKQQCQAFAFNTTNTLLFVGFQNSLEIYSFSDGTLKYLRQFKKHKEKISSLNYLKRSQNIISSSFDNSIIVWSKNLLSSSKYIQKLSQHNEEILCLVIEPIQENWIVSGSHDETIKFWIQKKSQWIVQKSIEGNYSPIWSLSMLESGVKLISLSSNYIMVFKYCNSKWILDQKIEHQTFGLRVCFINNETFIFQPEYQNFFYIYTLNKKGEYITKTKNPSDEEAEPDDNLFPALYNNNKSIVLLKNMNDVSIFKVQRNQNKKVMDFKINQIIKFQVTSVYGTLSDDGEYLITWDDHSKLIQVRKWIKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XSS0</accession>
    <name>A0A8S1XSS0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1360051</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000136">
        <property type="protein sequence ID" value="CAD8204147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="361494at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050349">
        <property type="entry name" value="WD_LIS1/nudF_dynein_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44129">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1225"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1360"/>
            <end position="1393"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1527"/>
            <end position="1568"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2345"/>
            <end position="2379"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2387"/>
            <end position="2429"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="3335"/>
            <end position="3376"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="444"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8204147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="3509" mass="413113" checksum="3301A9F752547552" modified="2022-10-12" version="1">MMKILKGQNMQKAYIFSKLEFNLKSTLETKQNKELKQQDNNQQQEISISALILEQKTDIEVLMYQDLKNKSCIDTILKQFVKLRDRIIANFASQQNSLVISALILQTQLYNSFFKLNLSSYQDIEQCKKEILELYQQYDQNQILNNIQFKNTQDQIQAENIEQNEYLILISENINELNNQIMNINRIIDNMQQLSKYVHQNCMGFEFFTEDHFQKYFLDNVKSIIKELIIQLQLDQLLNQFIQNNLDKQQNSGTQIKNIINFTLKDFATIFCYNQITSEKAVQIDKDYQELLDNLEQEMIVERKIYDLSKDYIVRQCFLFHLIKIQSQVPEKELLLLCQEYIKRIWMIEKDESVRAFLKNKEVIEIQKLLFSHDLKTFSSSMKEEMNQKLKELDDIEQAIRFEGNQSKRDELSKQLTNQYEEFEEFLDNISEMSQQLDITLIFLKELQKNIKQIKKKIDDLQESINEIGNDVRKLRGKKYDELLLIRKEKVLSQAYLLEVDSVYIPLKTQESIQLQGKSKTLFQVKNFLSCLLKRLDKTKQKGVNQFIYDEQQKCVMLIKGQAGSARKIEAYLWKQKLDEKDWIPIYKSLPQLKNPKFNLLDQTLESENYGFDKLQLKDFKEAISSNKIKLVLILDSYDEMKQDCIQSNLILTNRLIQDRNQVDQIKKVKFIITTRKEILTSLGYQTWFYGESLQTLKEVEIMNFDKQQSENYLKHYVKLSIKIRIKSSYDFVKQIKQQNLDVKEFLEIWNNILDQVDDEDGINENSTMLLTDEQIENILEIIQKQPAFQYVQEDQLIILKKELKDLWSAGLFNKTIQNLKIQHFLQTPFMLEIVVQTLPNLSQKYKGSQEIKEMFQKNFLILKSKENLSKMKLSKFIYLEQKKVQIIIEENTYKQSEIQQDYENQQDLKSLQIILEKLESQHFFEKYSITDISLIKNQIINISEDDLKIVIAALKMKIFTIYEFYQNFIECYHEQQLQKLRQLGKVENVDSFTIDLLQFSESLAIELNNISRLKLKNDYFDQNNEDDWKIQYFDDLEDDYRKLVRSSALINVKGKSYTFNHKSLQEFYVAKHINNLIDKLQFIDNQLTQKSVSYLEKSVFNKKEFNISLENYFGSLRILKENLKLKNENNLKLISIAKLSAKKTFKVASSNSFCLLSYLQVYLGEQDFSGIHIEKTTFKGLSFYKSKLKKSIFNEVIIDSCLFDQANLEEAKWTNMICSEKPLLQGHKGSVVVIQYSNNGKLIASADSQKVKKFWDDATFQQEGEISDISGLPKFLVFSDDDQLLFVGSDTNRIESWLISDLKKIIKSNCVINKQEKIIKMQLSQNEKELLAVDERGLIQFWDINLIKTNFNEKDGFILKSSDSKIKFISFFKNGQMLASVSDDKKVTLWDVIEQKVEAIIEISFPITYLAINQTSEFPVCVSNESKSENLSVWNIENINQPYLLRTSSKYHIDNIYFTTDNQKSIENLEDVILIQNSSNISEQEEYYQIINCTCIDFSPDENLIATSSEEQISLWSLQTNTPITSLENDSLVNVLRFIEQGKKLLSGNYDKGVKLWSVEKFQLINFFQISYTQNITISSNYDRMAIQTNREYKEVVIIKLDQLQFNSFDDNGMKVSISQNDQLFVINYNYTGTTYYVIKLDNDQQVEFESSKNNFEQCVFSHQSSLFASFNKDQQLQIWTYEGVKFSLKHEERFGYKISCMIFSFDDKYLAMTSQTDQLIIIWDFFKNSIYTLKESINEVGNYNYQLSPDICFSCDSLQVAFYNSDEIKIWNFLSNQTNSIFKDDNSKVRTIASSPKQIYLLHLTKQLLHSLFTGELTKCLCFIYDGKYLISARSILKLWDISNFPQIKLCAATDSFQGYNVEKICCLHKTQGILHCCSYRRILESSDQSKIEYQFSSDSRFLVGGIDKKVIIWDNDKNYQIEFSYPFPDIIQSVTYCQDQERLIVTIPSGIYILNIKSTNILEVIPNLKSAFYAKIFQNDQYLYTKEDKSVKIWRNDGDNQFTLIDYYNFQTTEKIVFSKDGKYLAKLKDDYFNKSIIIYPIDLIGKRRFILNKDQSTIRISNHSKYQLYGNDKLSKLSYFESDVTIDEFTELGYFEFSPDNENIVFQNDKNYTLYNINSKQNISQFSKYKDYQSIYSYSNSYSVQFSNDGKILVLAYEQIILVNITDINKPQFIGIIQDKITSLNPKTSSKYLASTLGENSIQINDMITRKFIKSIKINITRYYNLKDLVILLNDSKVAYLDYNKFKVQNLIDDKTEYNYSYTEDCDTIAISKDRKNLIFSSGNQVYFYQNINQQEFNKLEENIKFLNFSFSQDSKYLAGCGTNNIIYFWDFKARKVMAKFEGHSEKVYVVQISQDNSTLASCSDDKLIRLWNLNVNFNRITQDGHENKISKIMFSADSLLLYSSGAIDYTLKLWDMEHKVLIISKTFDYLINSFSITQNQDYLIITQGETVELWNIQYIQAIQKSLFEIIPGGCPSYNFKIKGNIQKIQLLSNDKQFVRQPCLHIKTEIWEKNDKIDESAILYEYEKIEIRLYQISQDFKYICSVDSQNELSLGELSLDSKLNLIKLKQYNNDKIINLLFSNDSKILSSVDSQNIVFRILDSDQLLCSLNLSIEPDDFFQYFTDDDKFYYTNFKNKIYLWNVNHLVQDQNFQISQLELNLENVDIQYFTISNNLKHLVIGLNDSQNNNILRVVELNQIIILKNIHQNSKIKLLLFSDDDMLLALANELNEILVYEINQFTITNSFQCHQAPKIKMQFFFYKVPLEIENNDEPQQMDNLDEQIIQIQNNEQENVEGQPQQIENIQEDKKDIIQRSILSLISFDQDYSIVINYLDNLNTHNQVQQLPNKSVNEIKQVVLLKIHRHLLQLQMITIIVNSVLFIIGIKISIAYLKTSKIVDFCFDQNYLVYSDLYLYDYQNDFGFFQREEIKINYNNYYPNEPFTFLKISSPNSILFLGDNQYMYCWKFCKEENQVQLILLKMTQYNNFDYTTMFLRQDSQIFCLMNNKILKLDFLDAIEKLKSCLNYYSQKDQRYEYQIKKLEAPNIQFKHCRFSSDLSLCVSIGQGRLFVFQSERNYGGSQYQYKEYFKYLILWNIKTQKYDTLIEPYTTKIGYNRYPILFTDVAVFIPNQNIMVSNKDRTIEFRNCQDFDSITTIATLDNERRIKNLIVSQDGKLLISICEIGIFKLWNISEINQITLQRVIYYEKELVHYRFTNDICCYSLYQRKEEKKYYLESLNLTKYPVIYAFQSFKYFNTFLIMQQETIIALGGGQLVLWNYKSNQNKSIIENNDECFTQIIFGNNSNNLVAARGCVIYLINEITEALNFTSISELKGHQGKIVCLSILYDNQMIVSGSQDKTIRFWSIPQLATVYILLGFIEIISKITLSPNCKDMAVAMEDGSIRLFELQFPENIHDLKLDEINNQSDYTHCYKEDKKDIIQRSILSLISFDQDYSIVINYLDNLNTHNQVQQLPNKSVNEIKHLLQQCVIYNWDQNKYSLFKNIKDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XST9</accession>
    <name>A0A8S1XST9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1360076</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000136">
        <property type="protein sequence ID" value="CAD8204177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XST9"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051438">
        <property type="entry name" value="RNF_E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46016:SF1">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46016">
        <property type="entry name" value="ZINC FINGER, RING/FYVE/PHD-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="21"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="233"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8204177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="418" mass="48928" checksum="5A013E913B1466BE" modified="2022-10-12" version="1">MIRERQFEVIKTQQVNQHLLCAICREVFYNPIRATCGHTFCGTCLVRWIQMKKSCPLCRHRLERNYQFDKDILATKIVGDIEVKCLRCQLWEGTLAQFKQHKKSQCTYISTNNEIHQDAIEIGDDDEEFTFTSLVEENDPQIRQQIIENIGQQILVQIDQSQNPEQQQLPQQNQDLNINHQDPPQETNINNNLISNHLQNANNNEELNIELSQKNADKIDTNKSPNDENQINNTSLQDDLINQNNLIQQVEQQINQINELNNQQQEQPQIPDSNSNEIEVKNQVNNKNHSINNDVIEKQKNIESIRVDNIQNQIQNDIKDDQSNQNKETNQSLIEQFKINDPDVEIINPTIKYINNKDLKNIKATNKFKINILQNNVKIAEPMMEEINDNLFFEFVDHMKKEMVKKLSDIQYYNTLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XTG7</accession>
    <name>A0A8S1XTG7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1350089</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000135">
        <property type="protein sequence ID" value="CAD8203902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XTG7"/>
    <dbReference type="OrthoDB" id="290175at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140933">
        <property type="term" value="F:5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03672">
        <property type="entry name" value="NUDIX_Dcp2p_Nudt20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000045">
        <property type="entry name" value="mRNA-decapping enzyme 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007722">
        <property type="entry name" value="DCP2_BoxA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044099">
        <property type="entry name" value="Dcp2_NUDIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23114">
        <property type="entry name" value="M7GPPPN-MRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23114:SF17">
        <property type="entry name" value="M7GPPPN-MRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05026">
        <property type="entry name" value="DCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01125">
        <property type="entry name" value="DCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="7">
        <location>
            <begin position="82"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8203902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="276" mass="32924" checksum="AF4ED0CACAB1CB56" modified="2022-10-12" version="1">MDQTRESLLCRFIVNLDQEEKKPDRLFFHLQNAYWYYLDFLNPEDKMSQTEFYSWLLNPLSEYNEIRGNLKHYLKQFKQYQKHIPLYGAILLNETLDCVLLVMNYNQTVYSFPKGKVNKNESGVECAIREVWEEVGYDISKKISDKDYLEFVCEDTGQPQRMYIICGVSEDHKFTTSTRYEIGSIQWVQIKDIQRGDSAYVYLNPFIQGLQKYIQHKKQLEKSKIKNTNQTNESDLISVMIEKTQEILKSFKTQLKQEQPRQLQQPQFQQFKLIIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XW35</accession>
    <name>A0A8S1XW35_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1410147</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000141">
        <property type="protein sequence ID" value="CAD8205769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XW35"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8205769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="195" mass="23398" checksum="A976CA3774CAA54A" modified="2022-10-12" version="1">MVFAIQFSVMGQDNKKNNVMMRVKYYLMNALIVNFDVISIMLIFLKEFFMNVLTILQIDQRGSGIKSYMNNVMMAILLMVTIEVDYMYQEKTYSTLNANTLNPITRQSNSQIKLIINIILKFHSLQQSFFFRYTRIFLDYSIDESHNEDYQIKLEQNYEKKQLIRFQNLNFRYKLNSKFLLIIILFSLIIFLTYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XWI2</accession>
    <name>A0A8S1XWI2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1410117</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000141">
        <property type="protein sequence ID" value="CAD8205713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XWI2"/>
    <dbReference type="OrthoDB" id="285741at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8205713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="164" mass="19418" checksum="45494439D9FAE420" modified="2022-10-12" version="1">MLFQDDSDFYIKPNAFLKLLKQGKPKTTEMCKRKPQNVVANKKQLNILHFSKPMFLLQIPEESKSNRNFSNNTCRSWNTQSHTPDRRKILLTSPKESRLGYKIQNNQDYEVPYEIHTQMPTSQINPKYVIQQKQGCGLSRLPNFKLQIINQFPNLRQQMMMKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XX60</accession>
    <name>A0A8S1XX60_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ADF-H domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1420125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000142">
        <property type="protein sequence ID" value="CAD8206013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XX60"/>
    <dbReference type="OrthoDB" id="10249245at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030042">
        <property type="term" value="P:actin filament depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11286">
        <property type="entry name" value="ADF_cofilin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017904">
        <property type="entry name" value="ADF/Cofilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11913">
        <property type="entry name" value="COFILIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADF-H" evidence="1">
        <location>
            <begin position="2"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8206013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="139" mass="15987" checksum="A9890007FA335D7F" modified="2022-10-12" version="1">MNVGTNVSDDCVTEFNNLKLGKQYRYVIYKLDKDNNEIVVDHKGARDSTYAEFVSHLQNESRYAVYDYHAQTDDVPPRQVEKLVFIFWSPDTNQPVKQKMAYAAGKEALKKKLNGLSKEIQANEPSEVEETEIKKIVLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y013</accession>
    <name>A0A8S1Y013_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Dynein intermediate chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T1470018</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein intermediate chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000147">
        <property type="protein sequence ID" value="CAD8207321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y013"/>
    <dbReference type="OrthoDB" id="366230at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036157">
        <property type="term" value="C:outer dynein arm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036158">
        <property type="term" value="P:outer dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000924">
        <property type="entry name" value="Dynein intermediate chain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001354">
        <property type="entry name" value="Flagellar outer dynein arm intermediate chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF7">
        <property type="entry name" value="DYNEIN AXONEMAL INTERMEDIATE CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0243">Dynein</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="571"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="582"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="613"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="644"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="664"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8207321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="681" mass="77158" checksum="908A65CA6BBE28A6" modified="2022-10-12" version="1">MADYYTYQRKRRDLGKPCNFQDSEIKIAGYTKTVAVIPNYVKRNPNHIDLDNIAEYSEHSVNTERVSTGDKVMYHKEGGWPAGIDPMEQQDQNKYRRRFEKDAAFAVAVKELSTTVEKCILQNNQIDLFEEYFLDEESEHQVENLSTKTLMLFKDQSEGVKRSVSEISWHPEGPIKAAVSYAISRFQQMPEGMLKSSFVWDLQNPNNHESELETNSPITNLMYNPKLSDQIGGGCYNGLVAVWDVKRGKQPVLTSPVEKSHHDPITHFQWLFSKTGTECVTTSTDGRVLWWDTRKLNEGPIESLNVTEGLNPNDPMIGATVLEYNTEAGPTKYLIGTEMGSLMVANKKPKKAVEITARYGLESGRHLGPVMSINRSPPNPKFFLTVGDWSAKIWVEDIKTPIMRTKYHGSYLTDGCWSPTRNGVFFLTRKDGWMDVWDYYYRQNEIAFSHKVSETALTCIKINSNGGAHHNAGKLVAIGDQDGTVTLLELCDSLYQLQFREKDVMNEMFDRESRKEKNLEAIKKQQDLMKKVVPKDNKAAQQKWEQRKAELIAEAEQQFSQIVKKDEEAKLNDLSEFSPKAQKKDDKKQQSPKDDDKQQQQGDGGQQQDQQDQGDKDGGVKDGDDGQQKGDDDQQKGDDGQQQDGDDGQQQDGDDGQQQDGDDGQQKGDDDGDGQKEGEQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y5H4</accession>
    <name>A0A8S1Y5H4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1550068</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000155">
        <property type="protein sequence ID" value="CAD8209606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y5H4"/>
    <dbReference type="OrthoDB" id="291414at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="468"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8209606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="574" mass="68816" checksum="65B934936AEDFC7E" modified="2022-10-12" version="1">MKKSIIRPSNQLNSPSISHYRSQTNGSGMIDQSRILEETSEKFQRTWNILESVEELKSVRQIVKQVTTEPCVQCECQLGGTIISGKVYKVSPMEFYYCNIGTRGQKSPLNCTMFCDGEFQIMISFNSPFPTKFNCDQVIRSKQWAVRHNGEDKLHLAVVARKQTDIKFLINFGQADSFRKLVRMTSDHERIYISDDIPKTPQFIVNQNKVLAFKKVNKFEMEVSRSDRFLQILKIRNQQKKSVLEENQMKNLEFKAKEWAHNEYKLITQALRNRHKKREQKYLWFELLYYIKFVDTLQTFLQQRRKQLMKNKMMVIGLQLRIKSFKDNRQKQRGDIQHRVIGDTVMSLMIYCKQTRRRLIHNCLVSVLPLLKLRASLFMFKKKAMITSGKLQLIKINLNQFIRNVRDYKSKMIQKWDQYTLKIHSIPSLQKMDKRFITWIKYLYEKGYQVYFQNQFITLLMRDRYRSHIREQREIKKYRLELKAAKLQLRFARESVDILTLRQRIFRLNNDIFAMQVKNQFFIHSDVERFVNSILEQSSFIFLDEDTNQLNQEKQSRIRVSLKRQKTMRISKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8B7</accession>
    <name>A0A8S1Y8B7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">peptidyl-tRNA hydrolase</fullName>
            <ecNumber evidence="1">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1570059</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the PTH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000157">
        <property type="protein sequence ID" value="CAD8209999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y8B7"/>
    <dbReference type="OrthoDB" id="311283at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00462">
        <property type="entry name" value="PTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001328">
        <property type="entry name" value="Pept_tRNA_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018171">
        <property type="entry name" value="Pept_tRNA_hydro_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00447">
        <property type="entry name" value="pth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17224">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17224:SF1">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01195">
        <property type="entry name" value="Pept_tRNA_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01196">
        <property type="entry name" value="PEPT_TRNA_HYDROL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8209999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="188" mass="20984" checksum="7EEE9EAFBF310551" modified="2022-10-12" version="1">MPYLLPKIRYAFCVQRVPNGTHLIVGLGNPGKIYENTRHNVGQQFVKHLANEFAIKMNDGSNGSIGSFQHIILFNPLSFMNSSGHQIKKLADKAKIATSNIIVVHDDLDNLPGRCKIKQGGSAEGHNGLKSIIQYMDDKFIRLKIGIGRPNSKDPAIVSDYVMSKLDYEPSQQAFKQGIMLARQLFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8C8</accession>
    <name>A0A8S1Y8C8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1570064</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000157">
        <property type="protein sequence ID" value="CAD8210009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y8C8"/>
    <dbReference type="OrthoDB" id="9984778at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051834">
        <property type="entry name" value="RING_finger_E3_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931:SF3">
        <property type="entry name" value="RING ZINC FINGER-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931">
        <property type="entry name" value="SI:CH211-59O9.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="423"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8210009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="469" mass="55134" checksum="BF5EDD1849A1CE05" modified="2022-10-12" version="1">MGNSSRHQNQQGIQSSQQTKQINKSESELIKYLAQSCSTFPSQITKNPEFKAQKVNCLRCDHILFDDGLGRTNKCEECQIPYVIKDGVITLLNHSQDIQCCPYGFPLQHLDSIVLRVNQNCINHISYGQLMSQIILPFFEKRERIITSGACFNIDQFEFRVVGCGSQLQGIVGTGTKIYCYESYTDRILYKLKIYTQRKNFQDELFHYFFSHPKENQLIQDSYIKINNQEYFVLQCTEQSGRLHLYTKMECISNVPRLSQVQFIILKQPIEFSHNNRNYAMQQVLNYVIHPYFAGLTRYLEKGQILRIGDFIFEVCLQEDYGFVIPNQTHIQITDQIDQRMNLQQFQSTQNYNIISRSINNQNNDRQDQIESLHRLLNTFIQLNENGTIEGNLGCEEHEIQQLPVRKINLEQIKQLDEDHMKCLICLCEYEEEDQVKTIPCLHYFHDDCIDRWLKKSRHCPICKNELEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8F7</accession>
    <name>A0A8S1Y8F7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1570079</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000157">
        <property type="protein sequence ID" value="CAD8210039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y8F7"/>
    <dbReference type="OrthoDB" id="305606at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="221"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="293"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="318"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8210039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="374" mass="42483" checksum="53CAF7D24311DCE8" modified="2022-10-12" version="1">MSLQQDMTDITDNGLSTNRVELVKYPILNPVIPPAQYVQMNDVKEAITIVKQEPETSRENFLVEYYKKILRQHLTILGLFLFGLMEWFANIVVDFGYYDNSFRWTFYFFVTTLIIIALGLPITRSIRAVVQHQNAIFHLQTILIGFVLIGIANQCSGRHSTATIWWIIITLLILICHSIAQFMLVKHNITHYYTMFMKFAIMGSITLNFIVGLCASSLDSSFPIAEAIIIYSIYYFNQLNEHLLKNPNELPEDVSIVQRIQIINKIQALFNQEAQSNQVIEQIKDTHCVINRYIAIVISSSVAYIIILILSIVTGNALFIVIVVVVSVSLLSVVLNTQVASRTLQQEDVVFAVCLTYLDLASPLKNILRGILSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YAJ6</accession>
    <name>A0A8S1YAJ6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1610003</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000161">
        <property type="protein sequence ID" value="CAD8210799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YAJ6"/>
    <dbReference type="OrthoDB" id="309994at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019784">
        <property type="term" value="F:deNEDDylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000338">
        <property type="term" value="P:protein deneddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044613">
        <property type="entry name" value="Nep1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46468">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46468:SF1">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="5">
        <location>
            <begin position="242"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8210799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="451" mass="53579" checksum="DA4B2216FBFD4F1F" modified="2022-10-12" version="1">MSTTSIQSDKEYKGIQKIQSLSPNKSHTYFQQTQKQHNYFNQPNQQKKAQNNNDNQKNSYILNKKKSPEINEKNNLSQQQNKNKSFLLEDSNYQIYDCISTFQLRYEKLLLCENLLFYYKSCCDYQENQNKEYKKMDGKLYQTNFDENKNPKFKIQEGSFSVNLSKQKFVLSENGIEYLGKSNTIKNSPIRDYDDGNNKRSKSAPNTKIVIIDVSTGLQVNKNYAIRNNKNELLSCWCKYNQQFCKKDNLILSNKGRLTSSIIDGYVMYLNLESERIYFKMQQRNRQEINRILLLPSTLTTNFGKDYSDQHSKLLFETELLEFQELNYELERIYSYIGMPVNQNNYHWYFLLFDLKQKICTIFDSLQQPNINMNIDQKLLSTLEKLLKIKDCKRELSLFSGQQFDGYSCGYHVCQFMKYCNEKQFQSNVKYNYNQSEITEILLKVIQQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YAU9</accession>
    <name>A0A8S1YAU9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1510075</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000151">
        <property type="protein sequence ID" value="CAD8208584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YAU9"/>
    <dbReference type="OrthoDB" id="10290560at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000586">
        <property type="entry name" value="Rab family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="195"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="206"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8208584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="228" mass="26290" checksum="AAE22855D349AD90" modified="2022-10-12" version="1">MSNDYNYMFKYIVVGDTNVGKSCLLLQFTENKFCDHTESTVGVEFGYKTIKINDQLIKLQIWDTAGQDSFKSITRSYYRGAIGGLLVFDFSRRSTFEDIKKWLKEIKTYSCDKIELVLVGNKSDILKKDITIEEVEEYAQQEKLEFYETSAKTGKNVDVVFESVANKILRKIETKQINLDDQMLGIKVNFNGKMKKKSKSQNNQSPYYNITLNGQQSESNKNDKKSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YB98</accession>
    <name>A0A8S1YB98_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1610049</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000161">
        <property type="protein sequence ID" value="CAD8210891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YB98"/>
    <dbReference type="OrthoDB" id="354826at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001468">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="19"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8210891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="425" mass="49397" checksum="4219C4A0B2434E3F" modified="2022-10-12" version="1">MDPKSLIDDLNDILLSEYYCFIEILGRGSFGIVIAAYSSNLDKIVAIKVTPYFEQEIESCLLQECSHPNIVKLHRVLKANNYIYLIMEKLIGQTLDVILRDRSLKEIEVQNIMQQVLNALVFLHRKGIIHRDLKPENIFICANNLVKLIDLGLGYQIVCRGIIGQQVGTPFYIAPEVIIGGEQTQAIDIFSLGIIYYQMMCNNKHPIWQDGVSKKDYYKLISQDFSIIYPYHLSEMAQDFIRNTICHNALDRMTAEQCLEHPWMLGEQSKSHPITNREIKLTYSFTQKFLIIIKALNIIKYMKDNCQEQMFYLKTQESDEINEILLNHHIPMTSRVQTETNINFNHHRVQNLKKSFALTKTTSHQDFTTLKNRQLNKYNATNRYQTQLDLHLSIPKVNSQKKIHVHLPSISPQQTKQSIFSFKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YCB9</accession>
    <name>A0A8S1YCB9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1630063</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000163">
        <property type="protein sequence ID" value="CAD8211399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YCB9"/>
    <dbReference type="OrthoDB" id="5979581at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001631">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="20"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8211399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="329" mass="38883" checksum="4E715F5941D72D5B" modified="2022-10-12" version="1">MARSQKSLSIYRENITFSGYLIQNKINKGGFGSIYKGVNKSTNQEVAIKHSQFDLKNEYQIMTQLSNVVGIPKVYSFEEQEKWSFMSMELLSQDLHTIKCKLKQFTLKCICFIAIKVLEILKQVHQMNIVHRDIKPTNLMVKSLKDPQIYLIDFGIAQDQSKGDTRQELEGTFLYDPISVHKRQKYRFLDDIEMLAYTLVFLYNGHLPWKKYGNEVLVNGDALKQKEQYINSKAMSQLPFADLFGYIKWNQHRKKPDYEFLINLFRNILRENKIIENYQFDWIEQATPQMKRQSKYLIIEDLNTDLNEEEQQCQGVSLLSELVYKFFKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YFG2</accession>
    <name>A0A8S1YFG2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1660096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000166">
        <property type="protein sequence ID" value="CAD8212123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YFG2"/>
    <dbReference type="OrthoDB" id="302584at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="127"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8212123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="191" mass="22544" checksum="9096071DC667C256" modified="2022-10-12" version="1">MQSQPKSGSIYHCLSPQNIKRAVNKIQCREQQSNPCVRLNNQDNRLKQFQTLLNSYMQSKSPSITKTIQSRKSLNEPSINNIARMSVTPQKSDISITRKQNTEHKEFLLCQSISSQTSDFGPQNQFKVQLQQIQQKVRERFKRYEEERKQFHEEKKLFIEEKKQLISRIQQLEQQVQELTKKKEALDQSFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RWL6</accession>
    <name>A0A8S1RWL6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0010292</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RWL6"/>
    <dbReference type="OrthoDB" id="2687506at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="57"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8131800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="225" mass="26519" checksum="E29016F8C1C3A010" modified="2022-10-12" version="1">MGSNTLNLLVEMKADLKRCNLNGSELDNVDIRGVNLNGAMLFNCKWKNLKIKGLNKLHGHRNKVCSVCFSPDGNTLTFGNSDDSISIQDNKKLNYIQYICEQLVCNIKKQKYFIVCWQQLIPFIFPYFIFNQLVLQIYHPIHLRGLISFLQQRKISLFWITYYGYEYFINNLGQLHNFIMNKFSCEIELDDKFIKQIQFQSQNDEGYNFFQYINQCNLKLSSRCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S1Z1</accession>
    <name>A0A8S1S1Z1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0020547</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S1Z1"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF206">
        <property type="entry name" value="HSF-TYPE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="3">
        <location>
            <begin position="6"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8133667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="280" mass="33694" checksum="F85B7EC61B52467E" modified="2022-10-12" version="1">MQEKQKPKLFLEKLFDLLESGEAYNIIQWTVDKDAIKIINRQQLISDILPNIFKQTSYKSFTKQMNLYNFKSTKDENGFTIFINPNFTQSSLNLTQIMAEKRTIKKSKKEDNKKRSELQHEHEQLKQRLMALFKYQVTLQNEIKTQLEIHKHLQMRVAIIKKCIYTRKENGLKRRRKLYNFLNSFFTHLKSSVICQKFLILNHDLICQCDDNDEEQCLDFQNSRSLYQTPYLTPRNLTIFQQNSIRQLQDLFYDEDNKPKSFLQLTPEQQKQFIETIWDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S4Y3</accession>
    <name>A0A8S1S4Y3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0030419</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S4Y3"/>
    <dbReference type="OrthoDB" id="299319at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8134662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="76" mass="9336" checksum="22298C566E644069" modified="2022-10-12" version="1">MRPIPWYVRLGIDVTIFTVPILMLASYPRLGTHFLNNRRRKYRRRNFDEIQYRNDSKLSSEEVINFRKRFGFQDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S5B3</accession>
    <name>A0A8S1S5B3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0030490</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S5B3"/>
    <dbReference type="OrthoDB" id="290880at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010992">
        <property type="term" value="P:ubiquitin recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849:SF0">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8134763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="544" mass="62177" checksum="F4037708EB00EDE9" modified="2022-10-12" version="1">MFAQSGKISEDKCKIHNNAYDCVSLNYTDRSQTFKACSQCKQQRTKEQFISFKEIQNKLQTQISNDLVGEFKLKLTPLFDETEQLRTKLLQLADDIYKILLNSIHNLFVNCLFKQLPNNLFTTQDIFDLMTPKTIDDFTSIIDQQISFQKGEDSSASLLTKVLFNDQEARQFLDALTETQGVKQKLEKVHSEFVQHKNDISKKIDQISKQSVVEVPKVDLNEPVLQFLIEKNKQEQEYLVEAIKEIQGVTDFLIEPNQEEKQENPQVKSIISINQQLLAYSSQTRIFIFDKTKNFQFIQQLDCENDISALRSVTLEDNKTYIIASVEENEESFQLQFWDWETFDKWKVINAHNKYIWQIINLPKNLIASCSEDETIKIFDAQTQQQVQTIAVHQASVNDIALINPNIIVSVSDDGTLVASNYLNGDLVGIVPQDSEIKSVIALDGIRFVTGNSSGEIIIYSFVQSIEIQQVIKAHDSVVRFLSRIDQKLFVSSSWDGTHKFISIIDGEIKVINGPKKNFSEPLLWIQDIGCLVASGGGILQKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S6V9</accession>
    <name>A0A8S1S6V9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0040226</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S6V9"/>
    <dbReference type="OrthoDB" id="303755at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8135443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="204" mass="23822" checksum="8CD2FA8E771340DB" modified="2022-10-12" version="1">MNSKHPVELLKKKIDPEAIMYKFRWSNNSVSIEPMTQLTPEMLVLVHDYELKQLGHDPQEPKPQKHIKQDTQIINQNNNGKKLEEQKREKRSIDKLEKVDKTEKIIEKTPEKNSEKQKQQQIQKDVDNVLNNNTGNGPHKPRKSKPCQVKQVRHDNGRVDFLVCFEDQTDQKWISLDEMKEKAPVAVCEFLLGKVKYGGTMNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S7G1</accession>
    <name>A0A8S1S7G1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">MSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0040541</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8136018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="442692at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904158">
        <property type="term" value="P:axonemal central apparatus assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031549">
        <property type="entry name" value="ASH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033305">
        <property type="entry name" value="Hydin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053879">
        <property type="entry name" value="HYDIN_VesB_CFA65-like_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058536">
        <property type="entry name" value="Ig_CFAP65_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057470">
        <property type="entry name" value="Ig_CFAP65_7th"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23053">
        <property type="entry name" value="DLEC1 DELETED IN LUNG AND ESOPHAGEAL CANCER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23053:SF0">
        <property type="entry name" value="HYDROCEPHALUS-INDUCING PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15780">
        <property type="entry name" value="ASH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22544">
        <property type="entry name" value="HYDIN_VesB_CFA65-like_Ig"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24507">
        <property type="entry name" value="Ig_CFAP65_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25249">
        <property type="entry name" value="Ig_CFAP65_7th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HYDIN/VesB/CFA65-like Ig-like" evidence="8">
        <location>
            <begin position="173"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="HYDIN/VesB/CFA65-like Ig-like" evidence="8">
        <location>
            <begin position="465"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="HYDIN/VesB/CFA65-like Ig-like" evidence="8">
        <location>
            <begin position="783"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="domain" description="HYDIN/VesB/CFA65-like Ig-like" evidence="8">
        <location>
            <begin position="1550"/>
            <end position="1646"/>
        </location>
    </feature>
    <feature type="domain" description="Abnormal spindle-like microcephaly-associated protein ASH" evidence="7">
        <location>
            <begin position="3075"/>
            <end position="3146"/>
        </location>
    </feature>
    <feature type="domain" description="HYDIN/VesB/CFA65-like Ig-like" evidence="8">
        <location>
            <begin position="3182"/>
            <end position="3262"/>
        </location>
    </feature>
    <feature type="domain" description="HYDIN/VesB/CFA65-like Ig-like" evidence="8">
        <location>
            <begin position="3596"/>
            <end position="3698"/>
        </location>
    </feature>
    <feature type="domain" description="CFAP65 seventh Ig-like" evidence="10">
        <location>
            <begin position="3730"/>
            <end position="3805"/>
        </location>
    </feature>
    <feature type="domain" description="CFAP65 fourth Ig-like" evidence="9">
        <location>
            <begin position="4057"/>
            <end position="4150"/>
        </location>
    </feature>
    <feature type="domain" description="Abnormal spindle-like microcephaly-associated protein ASH" evidence="7">
        <location>
            <begin position="4367"/>
            <end position="4442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="917"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1961"/>
            <end position="2033"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2086"/>
            <end position="2113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2255"/>
            <end position="2303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2802"/>
            <end position="2852"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="3312"/>
            <end position="3344"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="309"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1825"/>
            <end position="1859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="931"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1961"/>
            <end position="1970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="2003"/>
            <end position="2018"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="2255"/>
            <end position="2269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="2278"/>
            <end position="2303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="2820"/>
            <end position="2836"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF15780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8136018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="4672" mass="534785" checksum="76A22E00468A1326" modified="2022-10-12" version="1">[encoded data, 4672 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SCN9</accession>
    <name>A0A8S1SCN9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0060338</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SCN9"/>
    <dbReference type="OrthoDB" id="291630at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000184">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="207"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="FCP1 homology" evidence="4">
        <location>
            <begin position="376"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="83"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="546"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="791"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="85"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8137876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1039" mass="122920" checksum="4948095CB4C9E1E4" modified="2022-10-12" version="1">MRKNQTTIKTRPTRAISQAKDPKESQFPIIEQKPTKPSSVMRSSKKSLDFNRLIKNPKLNDSKLEEALEADLGFKRMEIKEAKLQKSSPQKPNLQLASETSQAEGEQRASTPPRKNRKEGLEFNVRSESFATLEPMHHAEETLAENLRIFIKIYDIMNQLLNSMKKNTTLELINQINQYMELTENNEFQYLEELFQEHVKQIRQIFLILRVGVILFIFQIVVLVNCFFDLHFYLSSISNIKNILLYNIQNLSHLSDILLEKLKEKPEYPLYLQLYSQLQFNINKKNNISKKHEYWTYIKQNIELLQNLYKSLTKSQRDLYQFLIIFLRSLQNYTIKESLRNLQEFISFYFTIQQSYQGLLGIPLICVAPSPYLPYTNPKTYTLVLDMDETLIHFTDQTGHFLIRPFTHQFLQEMSQFYELVVFTAGLPDYANWVLDQVDKNKNITYRLFRQHALQYSNQFIKDLSRLGRDLSKCIIVDNVPDNFQNQPENGIFIKTWYSDQNDTALAELGPILKSIVMKKAEDVRLALKEVRKMLTQNQVEVEYRIEMLTSELERSSQTLQQKSIECEEWRLKSLGLEQKIKELQAHIGSQNQQFEDLTENLKQKAIEVEDLRSKYNRVQFITIKGYEEKMEQLVGEIEKLNQLLMQKAQDCESSRQQVMTKIQELQEERQKCFHLDSFIKQIKSEKLEALHFLNEKNKELEELKERFREADQYRQKSEILESDIETIRVNYMNIFNQKLEEIDALQAQIDTQRLINENTIRIAEEKYAQQIQVERLNIQRQIKESVATSIVQMENQVATLNNLLEAKQNEIDKLKLQRHNIETEKMNSMLIDKNIKLQDQQIKLQQLERQLYEKANTLDQLTQQIQNGQLIPATSQSLLERSYKQLQDDMHKQNIELTMLQQRCLELQEDVQKKANMKASYEGQINTLLIEIANLKRDVYNKSRINSQHSDNQRLIQIENEKRVIKQKYEQDLKIAEDKIRYLLSIVDQRVLEQQLQYRGNLNPNPVLQQIIEESSSRIAQSNIYYHEDSDVLNDEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SFQ7</accession>
    <name>A0A8S1SFQ7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0060331</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SFQ7"/>
    <dbReference type="OrthoDB" id="309339at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="210"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8137862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="470" mass="55886" checksum="FBFCAC676F9E7E81" modified="2022-10-12" version="1">MNSDILLTCRFSLHNNKQIEGFCPYPQCNNFRLFCVNCLLDYHQDHTNECKKLYEYPQWAEMFNQINLENINKLESILEIFVILKQFKAWYETQYNPLIDNQESLNVQMFEQKITNIVNIKNITNIIDQSIKPQIEKLKTYLEYIMNQKGKINNLVSYDEFIQSNILSQSSLSNRSQRNYEETKSLYWAGKYQECINKCEAYQKFNITDVRFSLLKGDSYLELKNFEKAIENYQIAIEMDDSSYYAFGKIGDIMLNTGKLYEALKYYDDCTKINNQQQEYYFKKAKIYQKLNQFKQGLECVDHLLSLNSQHIEALQMKATLLQQIDLKKSGIDQFQQQLQFSAPHTQQFLQHLFKLGNQIQNRPLQGQILSKEEINFKYQQAYNQLEKNPQITLQICKEIFEMEKEREKNRDIDIDRDKINFQFIILSAHALKNLGIYQEVLNICELVKKIDPNNQEIESLEQIIYTLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SFZ5</accession>
    <name>A0A8S1SFZ5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0070309</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SFZ5"/>
    <dbReference type="OrthoDB" id="294435at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="386"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="434"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="458"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8138780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="628" mass="72411" checksum="465491528504367F" modified="2022-10-12" version="1">MFIEQYQETINQQLKLCKVVPITLNPKNVLRSLLAAEDKLDDEHLYQNYCKKQELIINNMNEENAYVIMNICSLIKLSLDKRKDASKEELQEIILDQIKTDSINWPQFKQIRWPEYLEYFSNLSKSLINHSFKLIESIVLDLTTKPFECSEFHQYMTDQLEQTMSVLAVEMSDFVRVPNSNSLYLELKKYFNSIIQNIHLYINRTSLFCNTQMEFLNETLTKYHTLANVRTYQVLQTETSQYIISQCHVSLKEMFFYRIEFLIFEKDLVKSIIRHFYEYSKLNLGVYQMSTLTQTFQDAIHMHQLLDENQSVQVNLDSKLFDQDGDCLKYKTWLYQKKSIQSQQMLTGSSVVGGSIGAGLGCLIVDSVDPKIPWEEKLKRAGFSSVATGLVGAAMRIPVIGTILSQALLFYAIKLTANNKVIDKNEKMKNLAHIGVQSSVGIGSAVAGQILIPIPVLGAIIGGTVGGVAMSLYSKFFVPKTKHSIKLMINDLINRQLDDGLFVYDEQVIKIMRINQQHYFGCKPEKYTDSQWLTILMVYLVNEVHYLSNIQGLEKIRELQEQMTKKPSDEIKCVKQIREIDDDLEQQEIISVKLEKSRNYIQQQEINTLQIAPQIGEFINGMIANFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SHA2</accession>
    <name>A0A8S1SHA2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0060472</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8138142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SHA2"/>
    <dbReference type="OrthoDB" id="270720at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8138142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="337" mass="39312" checksum="A2F2B8B508E8990A" modified="2022-10-12" version="1">MGPCSILCCKKDDNQVVEKQIITEINHALTTEDRKVTEFQEKITSIIKIQAHYRGHSVRKKMNQQESKQFIKESSLSNRICIIEAIKEEDQQLIKQTEKNQEPVELPEIEMVDGSKYKGQWMKGQRWGLGRHQWADGSVYLGEWKQNFAWGRGKLMHADGDEYEGEWQRDQADGFGIYIHSNGARYEGQWKDDQQSGKGIEIWKDGSKYDGEYKEGKKHGYGHIFFADGSQYVGQFFENQIHGNGEYTWFDGKSYNGQWKNNKMDGYGTMTWADGKKYEGEYKNDQKHGFGTFYWGDGRFYSGQWFSGKQNGEGEYTTTTGENRRGYWEQGKRVKWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJ64</accession>
    <name>A0A8S1SJ64_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1349 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0080029</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJ64"/>
    <dbReference type="OrthoDB" id="42525at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009784">
        <property type="entry name" value="DUF1349"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015987">
        <property type="entry name" value="UCP022704"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35332">
        <property type="entry name" value="REGULATION OF ENOLASE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35332:SF2">
        <property type="entry name" value="REGULATION OF ENOLASE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07081">
        <property type="entry name" value="DUF1349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF022704">
        <property type="entry name" value="UCP022704"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8139299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="189" mass="22313" checksum="2CCAA463B425E723" modified="2022-10-12" version="1">MDQTNLNWQWLNEPKDWKNQNDKITIVTEPNTDYWSKTHYGFIRYDAPTYVTKVKGDFVFTCKVNFSSKVLYDQAGIILFEDKENWIKASSEYHDENYSTLGSVVTNLGYSDWGYQRVSSQVKELYYRLKRKGQDFNIEFSYDGKEFHEIRVCHLHKETQETSVGIYACSPQDSSFQAEFSDIKLELIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SS37</accession>
    <name>A0A8S1SS37_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MIF4G-like type 2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0110313</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SS37"/>
    <dbReference type="OrthoDB" id="10252707at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005846">
        <property type="term" value="C:nuclear cap binding complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000339">
        <property type="term" value="F:RNA cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027159">
        <property type="entry name" value="CBP80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015174">
        <property type="entry name" value="MIF4G-like_typ-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12412">
        <property type="entry name" value="CAP BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12412:SF2">
        <property type="entry name" value="NUCLEAR CAP-BINDING PROTEIN SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09090">
        <property type="entry name" value="MIF4G_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIF4G-like type 2" evidence="1">
        <location>
            <begin position="476"/>
            <end position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8142740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="691" mass="81167" checksum="013CE1FEFC681D59" modified="2022-10-12" version="1">MLQIQHQTPPEINRIRQLICRAGDEGADTFKKDLQKIVDVLGEYYQQNKQEVKDAMIQSFIQCAKLLPLKSGIYATILALLSTKQITQSLTDQIILGLLEHLPQIAQEETPILGQTILKFLSELMNVGLLNTVSFVECLYDLEGGAENEQSPFYLQILLTTIPHAMIKAMEKNQIEFKNIIQNVEVLMTKRKTEDRLFHLWQSIRNFLNNQEKDAHTQIFQQFLKTSYPRPYLLFPDEMTQVRQLRRGFKIPIVKRTQIWRPQQILVLNDEIRSQNNNKTDIQIEITRQWIYETIDLFKTNRQQIVFQFQLYQNCIKLSPSQEAQFRQTLIFTLLNMVIEVIPRKDQLSTAFYSGLLIHLSQRNSDNIKEWNETIEQFIDLMPQNNILIIEQLSDCLSHYFCNSNFKLNWDKFFGKYNPDETDSINNYLVIMIFRKLFLLVSVDKITDLVPKNIIDWYNQLQDITKPLDIEQISLAEKINSYLQQKLNGIEMLEQLKLITNPNPDIVIQTFLECLLQCISKSITHLNVLSKRYLSFLLQPNIIKPEKLGEIMLQTIFRMWNHSVFHLKVYLKEFLNLEVISNLQIINWLSDIIKQKSDNFKIYNVLIAINDVFRKQTKLDQVQDCTYENVKEINTILSKMIEQEQDIIIQSSLENIQLQILIAFKSTNGSQSEKLLKEIKSTQIKQIIMSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1STZ6</accession>
    <name>A0A8S1STZ6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0140055</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1STZ6"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000099">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF803">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="10">
        <location>
            <begin position="108"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8145061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="444" mass="50498" checksum="2B976FF197F48BB5" modified="2022-10-12" version="1">MYKNQKYFWDALKVRDNRDPTYSVNLPSHSPKIPFKRPFQAETINRSMENNKGRYSEKHLDQSPNQKLKPVAQPAYFSQEGLLLTRNTKLPNYEPTRCSSNENGIIRAYAANTNQGIVRDYNEDRVSIILNIVKPQNRQHENWPRCSFFGVYDGHGGSTCADFLRDNLHQFVIKELDFPWNPYEALRKGFAAAEQYFQEFAVSQFNKGIPERSGSCAIVALLVGDVCYVANVGDSRAVLCGGNNKSALPLSRDHKPCDELEKLRIQKAGGKIYQTQQQQDDQQVFVGPLRVLPGRLSVSRTFGDIEAKLERFGGKPNVVVAEPELRSFKVQEDHQYIVLASDGIFDKMSSNEVVDIMTKELDSNSNIHQGCSIGVEQVLKESINRRTLDNITVVVVAFQGEQMKRLKNEIVKRNQIKKTVQAVLCNQEDQENTPGLINTYKRYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T231</accession>
    <name>A0A8S1T231_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein dpy-30 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0130438</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dpy-30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T231"/>
    <dbReference type="OrthoDB" id="417678at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22965">
        <property type="entry name" value="DD_DPY30_SDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007858">
        <property type="entry name" value="Dpy-30_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049629">
        <property type="entry name" value="DPY30_SDC1_DD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037856">
        <property type="entry name" value="Sdc1/DPY30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23356:SF16">
        <property type="entry name" value="DPY30 DOMAIN CONTAINING 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23356">
        <property type="entry name" value="DPY30-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05186">
        <property type="entry name" value="Dpy-30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8144862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="64" mass="7427" checksum="1A81229AC9CF190A" modified="2022-10-12" version="1">MSSEEERMKQLQSLPIRNYLDQTVVPVLLQAMTEVAKVRPPNPIEFIANYLMQNNPEKAQARQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T5A7</accession>
    <name>A0A8S1T5A7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0180334</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T5A7"/>
    <dbReference type="OrthoDB" id="9909019at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="132"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="8">
        <location>
            <begin position="84"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8148971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="283" mass="33969" checksum="0D4D21E51B8CB12C" modified="2022-10-12" version="1">MIAEVMPEALKKYFPLILAFFVLFIYFTFVYFVFIQISKQCLMEWIYIGIGTFSIIMLFWALYRTSRIDPGFIPKNTLVEYDETKQREYCLQCRIKRPERSHHCSKCKRCVLNMDHHCVWTANCIGLYNRKFFLLILFWGSVGMFSATLLGLTNIQALWNRIWEQDSFDFNKIKAGFIFLMTFSQFLNGFGLYYFFWNNFKLIAVNICTLDQIILNIEVQNKRKYHTDLTIYNLGFWYNFNFYFGKNPFLWLIPIGKPLGDGYHWDKKTSFREMTETTLQIME</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TGL1</accession>
    <name>A0A8S1TGL1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L18a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0210090</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8150937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TGL1"/>
    <dbReference type="OrthoDB" id="303909at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L18a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00273">
        <property type="entry name" value="Ribosomal_eL20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028877">
        <property type="entry name" value="Ribosomal_eL20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023573">
        <property type="entry name" value="Ribosomal_eL20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021138">
        <property type="entry name" value="Ribosomal_eL20_eukaryotes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10052">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L18A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01775">
        <property type="entry name" value="Ribosomal_L18A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002190">
        <property type="entry name" value="Ribosomal_L18a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL20" evidence="6">
        <location>
            <begin position="19"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR002190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8150937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="192" mass="22417" checksum="6DDF605E200DC6BE" modified="2022-10-12" version="1">MVKNSAQDPQDNSLQMKTRQYLVSGRRLPSKDDPNPKVISMRIFARNTVAAKSRFWWNLRRLNKLKPSHGQILAVQELFERRDTNVKTYGIVLKYQSRTTIHNMYKEFRDTTLNGAISQLYQEMAGNHRAQPQTIHILRTSVLTKSADIKRGKTNQYRGDSIKFPIVKTVPRASHKKFRTVFKAKRPNLYRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TL51</accession>
    <name>A0A8S1TL51_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Protein disulfide-isomerase</fullName>
            <ecNumber evidence="4 13">5.3.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPENT_87.1.T0230348</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>Catalyzes the rearrangement of -S-S- bonds in proteins.</text>
            <dbReference type="EC" id="5.3.4.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.4.1" evidence="4 13"/>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8153000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TL51"/>
    <dbReference type="OrthoDB" id="72053at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02995">
        <property type="entry name" value="PDI_a_PDI_a'_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02981">
        <property type="entry name" value="PDI_b_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000023">
        <property type="entry name" value="Protein disulfide-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000027">
        <property type="entry name" value="protein disulfide-isomerase A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000042">
        <property type="entry name" value="protein disulfide-isomerase A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005788">
        <property type="entry name" value="PDI_thioredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005792">
        <property type="entry name" value="Prot_disulphide_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01130">
        <property type="entry name" value="ER_PDI_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01126">
        <property type="entry name" value="pdi_dom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929">
        <property type="entry name" value="PROTEIN DISULFIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929:SF240">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13848">
        <property type="entry name" value="Thioredoxin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10 11" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039963210" description="Protein disulfide-isomerase" evidence="13">
        <location>
            <begin position="18"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="14">
        <location>
            <begin position="4"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="14">
        <location>
            <begin position="346"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="11">
        <location>
            <begin position="51"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="11">
        <location>
            <begin position="393"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR605792-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8153000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="483" mass="54202" checksum="049DB774CB891AF0" modified="2022-10-12" version="1">MKSLLFISLLLATSLCAFQEEDNVMVLTTDTFQDAIDTFKFIMVEFYAPWCGHCKKLAPEYSAAAAELKRIGGDDYVPLAKVDATVESSVAEKFSIQGYPTIKFFINGQAIDFEGGRTSSEIVAWINKKSGPPSTELNSVQDLERFLERVSSTPIIVFFGTSSDDNDFETFKQVAQSNDKITFAHTFNPESAEKYSVSGKIVLFKSFDEKRNNFDQSVTKDNLENFINVYGNPIILPFNDKAINIIFQQRNNAVILFTDDSDTGVAAFNAFAAVAGSFKDRIKFSFSKPNDGSGLFHRLAEYIGASTTNIPNVMLYDQLGGNGKYRFEGDITTESLRTFLTNFFDGTLTRYMKSEEIPATNDEPVKIIVGKNFRDLVINSDKDVLIEFYAPWCGHCKQLAPIYDGLAKKLLVNPNIIIAKCDATANEIEGVNIESFPTIKFWKNGQKDQIIDYNSGRDEANFISFLKENTSHQWVDLDRVEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TLY0</accession>
    <name>A0A8S1TLY0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Calcium-dependent protein kinase 1</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="ORF">PPENT_87.1.T0230234</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cell membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Parasitophorous vacuole membrane</location>
            <topology evidence="24">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="29">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TLY0"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020002">
        <property type="term" value="C:host cell plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020005">
        <property type="term" value="C:symbiont-containing vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000398">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000632">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000557">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 26" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17" id="KW-0969">Cilium</keyword>
    <keyword evidence="15" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1032">Host cell membrane</keyword>
    <keyword evidence="16" id="KW-1043">Host membrane</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0519">Myristate</keyword>
    <keyword evidence="12 26" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0564">Palmitate</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="27">
        <location>
            <begin position="130"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="428"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="466"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="502"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="539"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00060437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00068067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="EMBL" id="CAD8152774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="568" mass="65267" checksum="47A151F95C135E6D" modified="2022-10-12" version="1">MGATCCKSNLQTQDELSVQSAAFGDNDQGSQKRVVKQTTASKRQAYIPAQTFGLGLKQNLSDDGKEVYEEPKKVSMKVSKELEQLLERQNSDTDRKKILEQQSLQKMDQKLHINVDVFVQLKKGQISDHYITGKVLGEGAFGKVWQVTHKMTNLNRAMKQLKKTSILKEDKEKLFSEMNILKYLDHPHIVKLYELFEDDKNYYLVTEYCSGGELFDRIKSMNFFSEKKAAELMRQILSAVWYCHNQKIVHRDLKPENLLFVSESLDADLKVIDFGTSRKFETGKRMTKRLGTPYYIAPEVLLENYNEKCDVWSCGIILYILLCGYPPFSGRSESEILKRVKAAQLKFDHEDWAHISQDAQNLIKNMLNPNPAKRLSAEEAYNDKWIQNNAPSNVINQKALQNLQQFHAKSKFKQAVLTFMATQIITQQEQDELNKTFKAIDKNGDGKLSRQELIDGYTQVTNNQELAIIQVDHIMELVDINRSGEVDFTEFLIAAMNQEKFLSVQKMEQAFKVIDLDGDNYISKAELQNVMGDIDDEIWIQILKECDNDNDGKISLEEFSSLLQSKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TNA8</accession>
    <name>A0A8S1TNA8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0240240</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TNA8"/>
    <dbReference type="OrthoDB" id="308362at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8153473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="186" mass="21962" checksum="702C2C415E180E74" modified="2022-10-12" version="1">MKTTDYQKYLILQELYSVHSDRISEQFKQKKQQTRTQSCLFSQVNEVKCIIEEDTYAFVQLRKMLLIILSTSPQLISSGQYGCDHQSRLEKSKDNQLYFYLNDSHHEYSIELSIKSNLVPIILQKLKEDYSGIDLIHLGYIPYPIEDCDPDALLQKVLQEIQEQQQVLKILNKDKKIFFTLYKDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TPF5</accession>
    <name>A0A8S1TPF5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0250026</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8153778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TPF5"/>
    <dbReference type="OrthoDB" id="286637at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF35">
        <property type="entry name" value="LD22662P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="159"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8153778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="363" mass="42827" checksum="830B63238B49A0BA" modified="2022-10-12" version="1">MLKRMQSKEDIIKDQYMKTLTCFLQDSTLYDCLSINNLVSVIDQSFSLFDVFNVFIETHIDEVLFWNPDVAYYDGVFTQTDLIRIILKCYQNTINGIPNVWGNSKNQVQPIMEEEDEDRTSTPVSKTQTIDQEQINKLLIDLKTISVRDWFNSYGENLHQSSLVQADMADNLNDAMKKILKQGVTRIVVIDTESRVIVGILQQKDILAFLVKGFSQYFHLQLSQKSHKIEIINENKQQSEQHELEISYFSERILQLNDKLPFDTNVYDVFYKLIYVFKRNAIPIVDANNQYLGLIDRRDFLFILKYQVYDMLNRQAIDLLNFIRIEKSKYAGFCICNKELFHMKQTLKEWKTYFSHLEVVQYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TRG4</accession>
    <name>A0A8S1TRG4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0250198</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8154112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="687730at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051266">
        <property type="entry name" value="CLCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10579">
        <property type="entry name" value="CALCIUM-ACTIVATED CHLORIDE CHANNEL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10579:SF43">
        <property type="entry name" value="ZINC FINGER (C3HC4-TYPE RING FINGER) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="171"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8154112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="338" mass="38799" checksum="661F74540EA851CF" modified="2022-10-12" version="1">MNQKQQLQSMFANSSNQQQQQQQIQNQQQQLNFQNFSQIPMQQSSPNFPILSQPTHTNNNIFGGPPQQQPQQQFYAQPNLQPSLFGTNNTGNYNQPIINFQDDEMIEPKKEDAKQIANYDLNEKLSFEVKTLYKMGKLINSRTQYLPGIVSIKAQDQIITKNPENQRVGVDLICLIDKSGSMDGQKIAMVKQTLSVLLDFLSDCDRYQLITFESSAQRLTPLKRVTDGNKQYFKQIISQIYSGGGTTIGSATDLAFKQLKERKYRNNVTSIFLLSDGQDGNAVQRIQEQIKTVQEVFTLHTFGFGQDHXINNQIQSKNYDQRQKIKFKLFKYFISLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TSP6</accession>
    <name>A0A8S1TSP6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0260289</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8155053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TSP6"/>
    <dbReference type="OrthoDB" id="294979at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="101"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="232"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8155053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="478" mass="57198" checksum="81A24535FE38F58A" modified="2022-10-12" version="1">MSESQLIEKLQQYGSKKGKYENELLNSSQECSYKISTSPRFHKFHQSPVIHQSPANVSSSLEHYPFDKEEIQIKDPQGEIRFLLNIIDQLQYDLQIQYNDSELIKKKYQELQYQVQKMQSNVFKEQEQYETKQYCDQLERQNKQLSNENNTLKKNYQEIQQKYLDCQKDLDNLKLNASELLIQNEGLYSLLNSLKEKHQFDIDSQAKHFELINQKKIDDEINIIQKQYRREIETQNSISDSYKKKIEDLTNECEIKSKKLSQQQEINLKQELEIEILNGKLIRYQKLEQELVDIDTLYQQKNRLCQQLVQDNTKLGEELKSQKQQIQQLDSSFKLQQNLLEKERSDYVERIQQLQKQLDELQNVNNEFKKKELEQQRLLNKLSEYGDENMRLLEQNTFILDKTNDTETLRVNYKNLVKRYYQLKSENDGLVYSINQHITNSAILQRQLQNAIIEKNEVITNLHYTQQQLLNLQYSISH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TUR8</accession>
    <name>A0A8S1TUR8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0280085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8156010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TUR8"/>
    <dbReference type="OrthoDB" id="296767at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013887">
        <property type="entry name" value="UPF0592"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35397:SF1">
        <property type="entry name" value="ARMADILLO-LIKE HELICAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35397">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08578">
        <property type="entry name" value="DUF1765"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8156010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1002" mass="119166" checksum="D4B60B7A7C27BC3D" modified="2022-10-12" version="1">MGGVCGNHHHHHQHNQFAKSPIGPHRTGCSFMNPERMKFNIIFENLRLQYPTKSCHLSIKLAGEILMETPNHIDLDGKHAWKKIVETQIQANILELHEKKLEFYLLEEKNVIATLEIPIFDIISGPIHFDYSIGKGRLSFDMQMAQILQIDINPIEIDCSYVESIKDKAYVFNLRLVTRKMYFTSPNSESFYNPAYLRGFNSSLNNFLQEMMFRTTWNSSSELPIFSIELPSNEMYNSSLQVCIWSINKGNEFQSPGICSIKSLKEKCEKEVFIDQNLFAETFIALHKLIQEDDEEDFQMYQQFKTTVTKSLWCKGNKVGTIQCKFNVKLPRFLKQKLVGIRTENGCTLGVNICSSKSVAQIAEITNIFQKLYESMFKMQSLSHNDPNRTILQHQALTISQQLLIEVQKADKDTNSKLFYYKNQEDLFKAQEYFIKIAEQITKYIDKLDENLREVCYEILLVIIDRGEFRLHSLGYFEECKQLSKKQLELKNTVNLHYSEFMLQTLNWVLLKVPIKTSQQNERKFMMRFLVLSFIRIYNFKDQLLKCINKSNDPQLVEWRGNEFQLEEQDVFINEQVAMIFDWQTYFYNYLTQNQQYQLSQSMCDEQWKLTLGKRSLIYQYFIMDYCTYIQYILQKNNIQWQHVPGYKQLLKSFLSELKLKESYTASFFDCVMAISRNTNIINIIVMILFNKTNLYQSDQVIQVFDLLSLIINQCPQTLTVFDYPFFLNGIRIVLAQSEHAIAIATVLELIYTNFLKFPIEFRKAIIDLLFEPICFELFLHWSKTVRTVFMSFLLYRICHQYRNNKISVMDEELFEQQYLIASKPRKNFSFYENRKEEKQLIADYIYLKYTRFMMNIEQVKIQLKQFPIHKDLQVLQNLKQKLAQKNQYHQENQNIIIQEQRQDDKEEIPSEKQIIFERRNEYRNPTKKRTIILSDNKYKYLKVALNEFSELTKQYTRWRYQSMNMSGEELSPEEKGQAYLNFQVPQIKILVHYDQKEGRQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TVK3</accession>
    <name>A0A8S1TVK3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0280174</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8156188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TVK3"/>
    <dbReference type="OrthoDB" id="29851at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000029">
        <property type="entry name" value="Heat shock protein 110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639:SF4">
        <property type="entry name" value="HSC70CB, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639">
        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8156188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="550" mass="64504" checksum="7F1D26887EBB3B8F" modified="2022-10-12" version="1">MQEEYLGYGVDLGGRYTLLAKCQLFSADVVLNEGQRETENVIVFQTHERFFGNTAAHSYRSNILNSVRGFQKPHTFQYKGKSIKMEPEQQIGAFLRKLLRNCNRTIVISYPDFYTQFDLIRLWRAIQISKIKVLAAIPENMAISTYVNYCQNNVLQKEMMIIDFGHSKFTAFLLSQEQVICEVFDRNLGTFQMDNYLAEWIINKAKITIDKQSKYYLRLLDTCENIRKRLSANKDTHIDIEQLIPDVDFTYNLTREQYETIIQPVLESIQNLLFNLYHQTQNYQFNLIVTLGGGSRIPIIQQCIKTIFEGKQIQSTIHATEAICQGCAVSNTLITQRKEQLKKEQSTHHYLNLYEHYPIFIGIKTQDQTNNQMHQINLREYFVLNGECQKFSHCLTKQLDQFKSYEISLFYDPPPIGCQSLIKTYYITNTKIIMFYYDDYGVVQMTADNQIALPIQDQRIGLIEMIEIEDQLELDDSNVQLTHDLRYQCERRINEVKQQYKNINLSVPQLLKVGELIEESEQRVLDQSLTYQDVQNLMNEFEIQYKNIIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TXI0</accession>
    <name>A0A8S1TXI0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable imidazolonepropionase</fullName>
            <ecNumber evidence="4">3.5.2.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0290282</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-imidazolone-5-propanoate + H2O = N-formimidoyl-L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23660"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58928"/>
            <dbReference type="ChEBI" id="CHEBI:77893"/>
            <dbReference type="EC" id="3.5.2.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallo-dependent hydrolases superfamily. HutI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.7" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TXI0"/>
    <dbReference type="OrthoDB" id="194468at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050480">
        <property type="term" value="F:imidazolonepropionase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019556">
        <property type="term" value="P:L-histidine catabolic process to glutamate and formamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000007">
        <property type="entry name" value="Imidazolonepropionase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005920">
        <property type="entry name" value="HutI"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01224">
        <property type="entry name" value="hutI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42752">
        <property type="entry name" value="IMIDAZOLONEPROPIONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42752:SF1">
        <property type="entry name" value="IMIDAZOLONEPROPIONASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0369">Histidine metabolism</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="11">
        <location>
            <begin position="65"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8156988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="411" mass="45294" checksum="CF9C20F9DE256416" modified="2022-10-12" version="1">MLLSKAKQIVCISKTGERFKFANPDLCILENASVAINEQGMIVDIGDIDEKYQNYKIVDCRGRCVLPGLIDAHTHPCYAGDRSGEFKMKLEGATYMEIHNKGGGIMYTSRCVNQSSQEELEQILESGIKRAIRNGTTTMEAKSGFGLSTDGEVKQLLTIEAVSKRSPIEIVQTYLGAHALPPGLSEKQAEQDIINNQIPQIKKLIDEDKIHPEFVDVFCEKGIYELESTKNILQEGAKIGLIPSFHGEELNHLESAKLGADLGAIAISHLEHLSKEGIQAMAEKKVVGIILPTTQFILKLQPPPVREMIDSGVPTALATDYCPNAYCMSLPFTMSLACLNLKMRPNEALCAVTLNAAAALNRHKTIGSIEIGKQGDFVILDAPTWEHLVYQIADSPIWKVIKKGKIIYEYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TXR7</accession>
    <name>A0A8S1TXR7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0280229</name>
        <name evidence="3" type="ORF">PPENT_87.1.T0280230</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8156257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8156259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TXR7"/>
    <dbReference type="OrthoDB" id="307490at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8156257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8156259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="127" mass="14961" checksum="F48CE8DD1D97E214" modified="2022-10-12" version="1">MGSSCNSIKCEQDSIDYDIPEQEGQIKRITLNFVVKQQYFGFKNEDILQIENKSLYIPIEYQQNQILFHSILYQSEIAKSQTLESSGKYQPKKNNSLTQVNNKNIQKEEYNQKLQKSRLISYQNLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TXX7</accession>
    <name>A0A8S1TXX7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0290338</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8157100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TXX7"/>
    <dbReference type="OrthoDB" id="292008at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035726297" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="16"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="505"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8157100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="559" mass="64792" checksum="F2656A7BD89198BF" modified="2022-10-12" version="1">MIVLIILQLLYSAQNTKVSQPNITTYSEMIPSANFSNKVRQTKQFGYTKYPGVFAISLKETFDNNTEQSADLNGLEIEEKQFGTFSGCYSTQRSIGINFNLQLQLDQLITQPWMHFTDLATADNKFKAYIVRNDYSIFQIDISSGLGEIVLHPKNQSFKDLINNQTMVDQFYMPHPYLIDDEGSNKIYAITEFGGVSFSTDGNIGDIQMESEDIQLRKQIYNVHKNSRAQLIIVACGSEGIDLYDIQNNGKIKHRRQLTPEQLGIEDGIIIDVDSNDDETKIYLLDSESGLYIFDISDINNITQMMSILLPNTKCFDHYQNTFFLIAKTNTQLYYAVEIFVDFQTKSYYYNNLYIDEMIINNVNVFEHYAVLIGDDGHKIIYHSIYNKFINPAVKQQTYFQEDNLLKIKEYDFSKFYNSSNRIMIGIAKYAFKYIEITFEDPKITCLSNEIVSKSYIVTLNSTQCQNKTLHADNSPYSLCQLTHSFVYQSEYNPGNKSTYAQYTLTMYALVVGLIWLVIVISYLYKKWGYKLEFLKWRKQKLIVTQPFNQELEMPNQNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TZH8</accession>
    <name>A0A8S1TZH8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0290218</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TZH8"/>
    <dbReference type="OrthoDB" id="312578at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8156867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="146" mass="17417" checksum="87FCE034020D71DD" modified="2022-10-12" version="1">MKIHNTWFAQATYIGIIGAFVFATTRFTLPKLNIFENFMISIQITTQFIHFQVYASTFIFIHLILSIQRLQSLFYTFLDRLKELRDIYQSSLILISQLNQNKPKKVHLKMNQTSFIFLQQFQFKQLSSNLMFTWTIFSRKIINTIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U0P9</accession>
    <name>A0A8S1U0P9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0310055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8157894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U0P9"/>
    <dbReference type="OrthoDB" id="293714at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="96"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8157894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="165" mass="19858" checksum="42F2CFAB8B603BF5" modified="2022-10-12" version="1">MSQSHLKCTCQSYRQTQTLREDPGLIEKLKERQMELEEKLMDFDHKKGNLERENLMLEDNIKLELQGLKENQVIQQQLTEELRDNKILTYKKQNELHILIQNVHENEAEKKQLMNEIEFLKQSLLHTNENQIQLGKKEEHIKLLLNRISELEQNITQLKREVQFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U1N7</accession>
    <name>A0A8S1U1N7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Ataxin-3 homolog</fullName>
            <ecNumber evidence="4">3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Machado-Joseph disease-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">PPENT_87.1.T0330275</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Acts as a chain editing deubiquitinating enzyme that binds and cleaves 'Lys-48'-linked polyubiquitin chains, with a preference for chains containing four or more ubiquitin molecules thereby modulating protein degradation by the ubiquitin-proteasome pathway. Probably by regulating the IGF-1-insulin-like pathway, regulates lifespan. Regulates germline DNA double-strand-break repair and apoptosis in response to DNA damage by recruiting E4 ubiquitin-protein ligase ufd-2 to DNA repair foci. Interacts with key regulators of transcription and represses transcription. Acts as a histone-binding protein that regulates transcription.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Forms a complex composed of deubiquitinating enzyme atx-3, adapter ubxn-5 and cdc-48.1. Forms a complex composed of deubiquitinating enzyme atx-3, E4 ubiquitin-protein ligase ufd-2 and cdc-48.1. Interacts (via RRDR motif) with cdc-48.1 (via N-terminus) and cdc-48.2 (via N-terminus); the interaction with cdc-48.1 is not required for atx-3 enzymatic activity. Interacts (via C-terminus) with ubxn-5. May interact with ned-8.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U1N7"/>
    <dbReference type="OrthoDB" id="10063692at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01767">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.10:FF:000018">
        <property type="entry name" value="Ataxin-3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033865">
        <property type="entry name" value="Ataxin-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006155">
        <property type="entry name" value="Josephin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14159">
        <property type="entry name" value="ATAXIN-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14159:SF0">
        <property type="entry name" value="ATAXIN-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02099">
        <property type="entry name" value="Josephin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01246">
        <property type="entry name" value="Josephin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50957">
        <property type="entry name" value="JOSEPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Josephin" evidence="21">
        <location>
            <begin position="6"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="UBX" evidence="20">
        <location>
            <begin position="297"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="active site" evidence="19">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="18">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <feature type="active site" evidence="19">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="18">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <feature type="active site" evidence="18 19">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00063584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00069055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00082365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR633865-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="CAD8159691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="369" mass="42806" checksum="C0AAFC621F98892C" modified="2022-10-12" version="1">MNQQKQLIYWEKQGYDQLCGVHCINSLLQGPYFNEIDLATIAQELDRQEIELLGKTGHRYKSQNVAEDGNFSIQVLAEALKKLGDLQIESVDSKINQNQDLSQESGFICNSQAHWFSIRKIENIWYNLNSTNKRGPEIISDFYLSAFLQSVKENGYSIFVVKGVYPPPQLDNIDQNDRQKVLTTQFIKQVHDRRVKKKNYQLNIGGSDEIEMKKALKASKGEKYESTDEEFEEELQQPNKMQSTPKFQGQGIQFGQQKSQVNYPNFNIDPLDAEYRSIILMTLEQKFNIQLEEGLVILFQLPNGQNKQYTFSYDATVEDLYDFIFFESRQQSMRFTLICPIQKLQLLDVTQQLLDIGIDTMTQIIVKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U1Q5</accession>
    <name>A0A8S1U1Q5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0330013</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U1Q5"/>
    <dbReference type="OrthoDB" id="2942533at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="56"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="90"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="124"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="158"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="192"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="226"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="260"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="294"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8159215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="367" mass="42446" checksum="B4C165793A622F60" modified="2022-10-12" version="1">MDNVNANNSQLDNDELAINYQKSYFANNQCEVVGIRALQIETSEQADQLPIYQENFETLINKGQALHKLQRWNEAIEYYDKAITLNPQSSITYHFKGSILSYMNKIQESIQCFDQALKINPNDSVVLLLKGDLLLGQNKYTEAIECFDIIIKLCPADSEAFILKSKALHALTRFQEAIECYDQIIQISPLNSEVYYNKGDALCQLNRLEESIIYFNKALSINPNLSEAYYKKGNALISLHRYKKAIECFDQLLLINPKDRLMLYSKGYALTKMNRYFKAIQCYEQQIQLNPKDLQAYFLKSKVLSQSKKYNEAIQTLRKAYLISPQPFIMKTLADSLLLLGRKAEAKQFYLMAGYKKKFINKKLKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U296</accession>
    <name>A0A8S1U296_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0300220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000030">
        <property type="protein sequence ID" value="CAD8157539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U296"/>
    <dbReference type="OrthoDB" id="288980at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SPRY" evidence="1">
        <location>
            <begin position="297"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8157539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="420" mass="48539" checksum="2AAA05E99B877314" modified="2022-10-12" version="1">MLNMVKTYKQMNKPDDKQNVKKYSNIQYPECNLGGNHSGEILSLICLEKECQQNQILCCCICQEELHKGHQLKPLKLLLCEYDQKQKEFQSITLKQSEKDLLVNKINEQEIKSLEQTQDLQQQIVQKLNKIQDHQKQFFENLRKLVKLREIDDGNTTLVIENIMNNQKNSQQFRKFVKELLDALIIQDLPDQDLGYEELNKAFDFHIQDQSDHIKQLEKYMFTGVQQQIEFLIQKTSKSVFQQSYNFQFLPNNKHPQVEIIEQKIVKASNSTHGYKFAVMNPSLDKNQVTVFGFKLNNVHTSNWLAVGACHLSIVQSKQFGFAFQSLGHGGYLVSSNGGAWSSTTANQNNVVKCFKFGKGDVVVCTYDPKNEIMIFTKQKTKTTFKLDIPKSDNDLFPCVLFYYALDEVEFISSDAINKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U3R5</accession>
    <name>A0A8S1U3R5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Calcyclin-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0320113</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8158713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U3R5"/>
    <dbReference type="OrthoDB" id="164025at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044548">
        <property type="term" value="F:S100 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06468">
        <property type="entry name" value="p23_CacyBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000040">
        <property type="entry name" value="Calcyclin binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052289">
        <property type="entry name" value="Calcyclin-binding_UBL-bridge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037893">
        <property type="entry name" value="CS_CacyBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007699">
        <property type="entry name" value="SGS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13164:SF3">
        <property type="entry name" value="CALCYCLIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13164">
        <property type="entry name" value="CALICYLIN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04969">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51048">
        <property type="entry name" value="SGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CS" evidence="2">
        <location>
            <begin position="68"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="SGS" evidence="1">
        <location>
            <begin position="146"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8158713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="216" mass="25213" checksum="2912560413180958" modified="2022-10-12" version="1">MSELEELQHDLAEIQQVITTLKRKSNVDYLNNRIKYLENSIKILTPQKVEQPQQQQQQQKEQDNLQYQGITKYAWDQEGNKVKVFLNLEGIGQLPKENINSQFTSNSVDVKVKGYKGLNHRFSIKKTFDELKDKDCSIKVTNNSIVINLIKKDQKNWDQLNFKEKLIDTDPSKVDKQDPQASLMNMMKEMYQNGDDDMKRTIAQAWSKSQTEKGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U556</accession>
    <name>A0A8S1U556_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0330007</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U556"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8159203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="135" mass="15841" checksum="D255BAC3AC13B4B7" modified="2022-10-12" version="1">MFQEQIDIAQCSISLLINQINGDILDYASIQSYQFSYRVTLFLNTRNKRNRINFAENLNVKHVQIDEQRIVQLLVNILNNTLIFTQKGGYIGLQIQEGDHFALFLKFSIMQMAQKNQHQVELQIAYMILLNLELY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U5R2</accession>
    <name>A0A8S1U5R2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0310086</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8157956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U5R2"/>
    <dbReference type="OrthoDB" id="293886at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8157956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="300" mass="35028" checksum="5E2AA7F0756C6508" modified="2022-10-12" version="1">MDSRSTGTKRLISVGSLSNFERAEFISSKTDTKQRLGELFIYEPTKLEMFGLRQQIVSTRIPKFDHPKPMFELDYQPEKTLTHVASQTECQKCNHSLSRIVQPKQNMESLHLSAPSLQPYEKQEPKVIVPKQLQSHIARQKKKDLPPILQYSFIQPPPVFTQSAKAVREQKSFGKLSQGRISKKFDSELVKVIQQELSKQLHKIEDEIQEKGYSVPLKYQNLKRTKQMTLSKKIKLEKQSQILNEKKTESQFFMQKEKEKEIRSIIFFKQPEKSQSQIRLQKEENSNIIFGYSNRLDVQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UC69</accession>
    <name>A0A8S1UC69_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pentapeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0370001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8161747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UC69"/>
    <dbReference type="OrthoDB" id="10486437at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8161747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="327" mass="38224" checksum="B5B9E298BA58C30D" modified="2022-10-12" version="1">MALKKMKDSKLDDTSELTKQRAAFKALLTQTEQMMKKIWKVLSQSIKYVYDQIEKENQSYLNLINENTNLAESSYTDIEKLVNIVERSTLNDWNAQKNSYMKELDQTKSWWDQHRKGFIEKSNQGIEYIQKLVKQELEIISLFFEMLTYLQAIDESLYKKIHQILTREKVSDILGFLSKTNNQRFFESLINTQANLKDVKKNSVEYFGSYHKFNKEDFSSETYEKARSDLIKGMKDNKGIIDFIKFLVLLTSIDGKFIQCGSNALNLNVDMRNESLNNIRIENTSLIEVNFVRCNLSGSELDNVDISGLNSNRALLFNCKWKKLKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UG34</accession>
    <name>A0A8S1UG34_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0380205</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UG34"/>
    <dbReference type="OrthoDB" id="305312at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8162719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="244" mass="29592" checksum="D277992204CCE72D" modified="2022-10-12" version="1">MENPLLDSNIIDKNNRDYVLFAKVSKNDETLRILIEEMQLKIKFTSDNKTYLKCQTCELLYVLQYHNNLQMSIFYKKHQKNQKDNSSNNCQQCQQFINGECFKKCYKCNSKQIICIQNEQQRLQCQICFSELCKNCDSNLEIFQQFPDTCTQKNVTNCQRYFYVITMIIMSFIFLPIFMVLNFQKYKFQCQFLQFHQYILTKYKPVIILFFYQVFLIVYLVKVFMILINFVVDKNKERIHYYFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UG40</accession>
    <name>A0A8S1UG40_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0390016</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UG40"/>
    <dbReference type="OrthoDB" id="300835at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="145"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="491"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="551"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="679"/>
            <end position="727"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8163073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="744" mass="87813" checksum="BBA170EB773D0B76" modified="2022-10-12" version="1">MQNQQLGQKLSNISSMIQQYQPLPQNTSTITSIPPPTPQSIKQPQVLQSLINDQKQFQQDVSGLIAKLDQMQYQNSPRRNQPTINGQSQVQPFQTQPDFVVQPTLSSPSSRCAPFVKQQDTKKAKEQNTSILRQAEQLEYFIQANNELKKALAQSNNKITEQQKHLDQLIKQNDEQKQQLITNEQNKIIQQKQYNDQIQNMQISHQRELQQIKNNENEQKQKIESQWQQKYNLKSNELNQATKLLNNKINSLQKDVNDKQEQLNSLIFQTKELSNQLEKRVQDLSVKEELLSDKQFELERLQNQFNQALEKYEHNIKDMIQNHTQDMISQDQVIDELKQHNIILSQQIIKIEQQNSKDIENIKKNHLKELNDKLDEIVQKNLKEKQQIQNHYDEIIYKLKNEKNNLQQTKDQQQLFLEQKVQVLSQEYKTAVETVKKQQEKILMLNDQLSQLEIQAKNFNRENINQIAYLNQKLKEMSSIQPQNQNINYYYNELKTQNLDLRTQLEEKKEKLDELINNLNQVIEINFTYKTQLSEFQEKLSNVSYNNEKENEAYFNQIKQQKITIAELSKKVDQYAKNEFNLNNQIKELKNNIEQLGNLKLGNKGDNFDKEKELNKHLIQKTNEYKQQIVDLENVLQGLQEELQQKNQLIFIHEKTINELKKNGKEIKQQIMSSQMSGIFLNEKQVQEYESQIMQLKDETNSQKVKIESLQSQLKQVRKDKAHLSMTLMNSGMLNIQSSVTEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UG67</accession>
    <name>A0A8S1UG67_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0390173</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UG67"/>
    <dbReference type="OrthoDB" id="341421at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10527">
        <property type="entry name" value="SET_LSMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1410.10:FF:000035">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1420.10:FF:000020">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015353">
        <property type="entry name" value="Rubisco_LSMT_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF137">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09273">
        <property type="entry name" value="Rubis-subs-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="45"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="2">
        <location>
            <begin position="163"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="Rubisco LSMT substrate-binding" evidence="3">
        <location>
            <begin position="394"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8163344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="572" mass="67789" checksum="38CF623901E1E39A" modified="2022-10-12" version="1">MPPKSRKSDLISNQLKEEGNTELLNREYYKAIILYTKALQFEENPICYNNRAQAYFYTDELELALEDCNKALQLNPNYIKAITNKAQVLYEMGYLQQAIECLQSINNHTPESQILLNQYQSQSLKNILDQGELERQKTLLEWLKQGKALFPKIKIECYTDTYRGVNAKQTINAKELILFIPKSHMITLEMTKEIPVAKKMIQFRLDLLSPKHSFLSTFLLQEKYRPNSFWKPYLDILPQSYPSFPIFYNNNDLEWLKGSPFLKQIKDKLTDLKKDYNDICAVAPEFKQYLFHEFCWARMTASSRIFGINIKGVKTDAFVPLADMLNHKRPKLTSWCYSDEKQGFIIETDEKIERGQMIFDSYGRKCNSRFLLNYGFVVDDNDANEVIVTVIADLNDPLLQLKEESIEEQLQWPKTFRLMMDTDEPTIMEFMSYIRFLVIRDETQLQLLFNQRESKYFKPTKTQPLSLQNEFDMWEMIRRISNNALQQYATTFEQDKEILQICELTINERNCLILRMGEKEILKFYQQLSENMKQLLSNFNQLEINIFCQKEENCKYLNYINKLIIFLNQDDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UIR0</accession>
    <name>A0A8S1UIR0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Endoplasmic reticulum-Golgi intermediate compartment protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0400294</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERGIC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8164244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UIR0"/>
    <dbReference type="OrthoDB" id="270930at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045888">
        <property type="entry name" value="Erv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012936">
        <property type="entry name" value="Erv_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039542">
        <property type="entry name" value="Erv_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984">
        <property type="entry name" value="ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984:SF25">
        <property type="entry name" value="ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07970">
        <property type="entry name" value="COPIIcoated_ERV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13850">
        <property type="entry name" value="ERGIC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum vesicle transporter N-terminal" evidence="8">
        <location>
            <begin position="4"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum vesicle transporter C-terminal" evidence="7">
        <location>
            <begin position="131"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8164244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="320" mass="36554" checksum="CB6F13BA7565E5C3" modified="2022-10-12" version="1">MKLLKAIDLYGKVPKGLAEPTSSGAVVSIITLIFLGLMIMNEMIEYITIDVQSEIIVDQQLSKDRVQVSFDIKFVRAPCDFLEIDQQDAMGQSLSQQFMELKYYRLDSSETRIGEYIRNQNNWIVIEDARTAVAEKQGCEVVGSLKVNRVRGKISFGPHRSHSYIGAVGNLHLPLDYSHKFVSFTFGDQNALKKVKSMFKQGQLESLAGTQRIKKYELASQSMQHEHFINIIPTHYTLLNKQTYSVYQYTANHNEVRSHNYANVQLRYDFAPTTVTYWQTKEDILHFLVQICAVIGGIFTVSSMIEASVYKVMRNVLKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UJM4</accession>
    <name>A0A8S1UJM4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0410142</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UJM4"/>
    <dbReference type="OrthoDB" id="366230at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005858">
        <property type="term" value="C:axonemal dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:003559">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:003672">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF12">
        <property type="entry name" value="DYNEIN AXONEMAL INTERMEDIATE CHAIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="113"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="321"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="75"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="117"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="132"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="150"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="330"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="340"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="362"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="374"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="384"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8164655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="890" mass="102559" checksum="C6EA96DB3CAB9FFB" modified="2022-10-12" version="1">MQSIGSEGLKAGVTKSNIRSNVTMQKRPINLGGGKTQLGPPKTNMDTQQSAADNIVVKYKDTVVTPKELVTKKFLQLAEQQNREEEQRLKVLQKQNEAKVSQSVAESNVKSFYQSADKSSNSDVKISSSNNPERSSFMESKQSEGTYSDSDSKRDQSEQQEKKTKQKKGLKEEDLEENIPINLSETCTMQLLFIPSSFINNEQGKKSGVIQKTQEYEKYMVEKIGSDNYTKRHAQTFNYNQKHKIQQSNKIEKNEYGAFAAIWDLYDASITDQLNEFELLQEDIKSSIEKEYKQHLKNPYFLLPTELEAIKIHTERPVLGKDYSEKQTNKTKTGINTNKQSQPLNSLSGGYTNQQLDEKMQDSSSSHTGTKNLQSQQVNIQQQVTRSHGKTSNPMPEQQQKQYEMKQDEQDLKEEDYMKELVKRNNTQMSEAEQVIFQSNLVQTNLKYVERILNQNLFHKQYIQYRNYPEVKFEKMTLDEDLKQRGGAFKRAFQNKQEDEEVVEKKKHEPIVQLFQYKCALSDQCKVTQADWNSVNKDLLAVSYSNQQTSEGHIMFWTLKNPTYPERIITYPSKINCCKFSNSQPNLIAAGTIDGIVAVWDIRRKSDKPVTENKELPGKHSDSVWEVQWIGKGAKGTDKVESLVSISSDSRIAEWSMKKGLEYTNLMNLKRVVQSSQKENLQEGVNFRLSAGFSFDFLQGESSMYLAATEDGTIHRCSKSYTEQYLDNYFGHSGPVYKVRCNPFMSDIFLTCSADWSCKLWNWREELPKANFQQQNLQDEVLDFEWSPYTSTLFASVCKDGRLELWDLTKNNMLDPFATLMPQDQQIWAAKTMVRFAQNSPIIITGDAHGEINTYRLYGYEDNDPLVQQERLEKLFYPTGYVKGQKEQRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UKA6</accession>
    <name>A0A8S1UKA6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0410103</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UKA6"/>
    <dbReference type="OrthoDB" id="310174at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="163"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8164577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="182" mass="20882" checksum="C801EAF7B23DAA3D" modified="2022-10-12" version="1">MDPIFYTFLGMGLGFIGSQVVMNNFFKNKQNTNPQQNQATKKYLPSTPDMKKAFQQNQIPLQIKRTIELDDQIRKKVKIQSSPAQFDKDDELLMAQDQESNKSHQINNSDHNTLPQTPNFQNKTQIQSNIKLFVTADQIQQIVTSPLILKSNKFSASQNNHPEITQSISQSEQQVENNEKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UKT4</accession>
    <name>A0A8S1UKT4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0410205</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UKT4"/>
    <dbReference type="OrthoDB" id="290301at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8164773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="201" mass="23399" checksum="A868F966585C09C5" modified="2022-10-12" version="1">MSTGLTKMNPYCQINRVQLRNGSVGPKIQPKRRLDSDLNSSQITPSYKHTPTNFERCNLDVFEDKSKPTDSSMNSTQLRKSPSFRMKNYRHITPGARDDIRRQTNFQSSAFKQRVSAMELLQFQLGTDDEQQQIRHTQPIKKIVATPSSSSQTTSVPKYNIDQTILEDNKDANLEEVHFFQVEMQQKYKKWLENIEKKLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UZG8</accession>
    <name>A0A8S1UZG8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0520112</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000052">
        <property type="protein sequence ID" value="CAD8170045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UZG8"/>
    <dbReference type="OrthoDB" id="305493at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="31"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="139"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8170045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="336" mass="40518" checksum="0A0D77C987F5A3B5" modified="2022-10-12" version="1">MVINEERTKSVTPEKQLETTPHLSSILKSRIKKNEENANYLNNNEEQLNNNQIKSIELKFKIKLQEQENTINLLKQHIENIENKHKENLSKMRDQLIKAQDEIFKKQEEQIHEINKLRKSYDDKIAQYISQVDSETQFNNSLQSKLDELRNVHQIKQQESQQTISQLRAEIERRKFQEEQILIDAENQQIKLKKEIQQEFEKNITIIRNDCILQIDEFKQKLTGKVEQIQSLQQQIQQLQNTLKEIEIVWQKKLQYEQQQYEELKNKYTQNKDECENLKKELKNLQSEISIYQKENELFQKEKLSLTKQLNQQMEKNEKMDRFIYGSKKSQTKIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UZQ2</accession>
    <name>A0A8S1UZQ2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA mismatch repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0510201</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the post-replicative DNA mismatch repair system (MMR).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000051">
        <property type="protein sequence ID" value="CAD8169693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UZQ2"/>
    <dbReference type="OrthoDB" id="10252754at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032301">
        <property type="term" value="C:MutSalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.10:FF:000019">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003734">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF148">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="6">
        <location>
            <begin position="978"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="25"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR037677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8169693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1111" mass="129967" checksum="6D30A486E2D21035" modified="2022-10-12" version="1">MRKIQKDENDDMFVITKNQKKKSTKKETKKPGKLKRLDEEYQPEQEKVSSESELQPSSDDDKYNKKKPHQSKSITVKKDKPKSKQKLKQFEQVEEEDNNNNYEPVEQPLAKDAEEFFKTAELLLPSFVQPEFIRDAEGRRPYDPNYDPSTLDIPIAQYQKLSPMFRQYWNAKKAHYDSLVFFRCGRWINVMYNDAIIIARMFNRHLGFWGRDRPCLTVYDSQLPIYQRTLLEKGHKIMLVEQLEKADVANKEDGEIVKREITQLISRGTLQDLNDMDSYESRNLLVLVCSNAPVNLKGHKYVYGVSIVDCTTNNFYLDQFFDDEQSNQLRSIIYKTKPLEVILSKATPEIEKMMKTICNPIIITSKKEFKECEYIFEQLKVEYLEMRKTQDKKNKQSINSSFEGIILPSDKIEPENQIETNINQGLYKDQADDIIIEQSNINGDYVLSDEYPSLFVELEKQYYFEKKLAQDEDESTYYSYYFTLQSFYIQLCYMRQLLIHTSVYRRGKFQFLDSNFNQKLNLYLDSQALESLEIFDVNLQTKVSSKGSLFEYLNKCVTPFGKRLLIKWVQSPLLNHKHIRERQDCVRDLMDFIEPCDEFQRRIKSIPDLERNIIRCFNTIHSHKLKAVPISGGESFGKIKLKEITQVIKHIKAASETLKVFENYLNKFKSNKLKKILSYKENASILNSALKELEKCISIQDGEPQPLKGVSLEYDRAFDKMTDIIDSLEQELKKWQNKFKCPQISYHHGKIRYQIEIPDKFLESGQKPKELVITSKKKGFQRFQTDFIEQQIFNLKVVEDELSQKLIPFINDYFTKFYSYRKEFLQLISTLSEADCLISLALVSKQYQFNTFPQILEYSPDEDEDQEFILQEAYHPCLLQNRDINWVPNTIKFINSVDTLLLTGPNMSGKSTLLRLIGIQIILAQIGCAVPAKSFKLVPFDRIFCRLGASDRMLEGKSTFFIELEETKTILEHCTSRSLVIIDELGRGTSTYDGVALASAVLRYLSQKIKPKTVFATHYHILLDEFALCKNIQQSVMKHYQEMDRVIFEYKLISGVAEKSFATNVAKIAGIPNEVIQNAKKMEEKITKEESKINRNREILKKFNQIIQQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V137</accession>
    <name>A0A8S1V137_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0530067</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V137"/>
    <dbReference type="OrthoDB" id="307006at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="361"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="102"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="295"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8170454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="438" mass="51647" checksum="4ABA0B45EBCDB5C5" modified="2022-10-12" version="1">MYSNRIVRTKVSSDFQNTCSSSFKRQQFHTQTGSISPCRITADNFCTAKTYTPAQSQNDKGLKKVQIEAFIAEIEKLNDVLKKKVNLIHDLTVKLNTNEAHLQEKRDEQQNLKTTYQRQIEEQQQKIEELLLTNQQLKQTNIQQQQELENLFLQIEQYNKESQKLKETLKNSQTIYNEVEVKQLRVEINNQKQEITRLQIQILEEKKEANLLKLQNEELNNQSQLKHLENCLNDQQNTIDNQKQCIAQLRKTINLLEQNISDQFTSENSYNQLVNAFNSYKEQQKESETKLHKLLKSKESEIMDLKDNFQSQQVQLEQSNLLVISLQNQIEKLKNMMKLKIEDFDLKKFENDSVFQQPLNQHNKNQSKKISKDHIGTPQNHASQHQQINFIKEQCKHIPMSSQNFEKELSSYKNKMNSPSNIKFRQEVKEGIFNKKIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V170</accession>
    <name>A0A8S1V170_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0530087</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V170"/>
    <dbReference type="OrthoDB" id="300915at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="13"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8170494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="214" mass="25201" checksum="9CAD85B16BEC2A1B" modified="2022-10-12" version="1">MSQRKKKRDPTPTRVALVCLKTTQNNIQINNQSLLKIFSKHGSISKILIFERGQLLTKVFIEYDEVNSAIDAIKYLNNTKILNQISCNVYHSRLKQLKLDTVPYTKGLDFTNLPTNIIEQDEQIEQQLENNDKIFNNNEWQDFQFENRSTDEVSDEEEPQFSEEKMKDIITKLNQIDEEINQTIETKIVTALDKLRQTQQFHKQQENQLIQINQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VAQ4</accession>
    <name>A0A8S1VAQ4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0590204</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000059">
        <property type="protein sequence ID" value="CAD8173593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VAQ4"/>
    <dbReference type="OrthoDB" id="292483at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="238"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8173593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="744" mass="87542" checksum="E958C17BC578F786" modified="2022-10-12" version="1">MIQFFVPNLSSKEFQIIIIQVILCLIFYVLRVKKINNILAQLQEFFNLSEIMNDLVNLMKVLLVIVSVVHNFGCLWHGIAHFNSQFSWLDAYNLRDQQNGSKYNVAIYWATMTMTTVGYGDITAKNDLELLINNLTMFIGSIVFAYSVNSIGILVTNIYKNSIEYSKTVRLINKFMIKNKIEFDLQTKIRSYLEYIWKEEQEQNDDQVGDVISKLSKQLQEELQFQLRGNILRKCKIMINTFSESLIKNLLQFMEEQSYSPDERIISVNELDDCALYIITKGDVELIFEGVEMKEKIKRNSFKNYTQFDSFGEFSFFSGNPRTATAISRGFTRVFKIKRQVFLDILKCYPDDQEKYFVIREQLNFKDYVPLQLRCFSCQSNTHMIIDCNYVHYCVDKERVIKQESYPIQQERRQGHQRYKQKFNSRINLNINQVKAANYINDISFKITEDYESPTQAMYDIFEDVEIDDQNQNIKSLMDFKQQSENNQDDDQSQQQSIINFKNNLSKTALQTLQTAGFGIQNIQKKEVQMIQEEEDSSDESKNQIEPIYSKHNIMNSNLRQARKSMKSSKRISTDNNKHYRPSIDMKIIGNHIQSDLNIKHQSNVSNNIKSNKSQALRTLIHQHNPTIITTLQNLPQSTFQVNQVIQGFEKMQIYQFYQPKYNYDQVIKRYAKLQKFFGKKRVFPEFSVYSFFYEAIQKGAKLRRIGEFQKVQKSPRMLNLTLKKTLRSNYALSRQLGVQNVMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VAW1</accession>
    <name>A0A8S1VAW1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0590234</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000059">
        <property type="protein sequence ID" value="CAD8173653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VAW1"/>
    <dbReference type="OrthoDB" id="292402at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="358"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="390"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="464"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8173653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="510" mass="59494" checksum="71D29CE83AFEB427" modified="2022-10-12" version="1">MKLAMAPYRRRSSKSLQEVPSNAEFERQQSNGSLRIDPGPINIEVRENNTKVVDWIVLITPICYTFVILFLISWIYITTPQIRYPYIAHNILQKAHKIQTMYPIVDITEFSQFSYHIRGCPPDFEEATLGIWEGIENGNVCDRSNKNCELVQSMEPQRLKLWKQKIYCIKYDKTAQWRNGNQCQKQYKACSSYICVKQQLQCPITDLQTIHQQGDIQFGKNQYKIVRDERQTPLLFFNISSSSTCLNQNNQPQFKSKEYYPLSKYPESGCDELGDYTSITNILDSGMVMEILKENDIPLEQLPHFDEYIQNTDEYQLQILRGIKLNEINECKKLNTEVFNNSSKKSFRITRIMRRNNLPLITGCVILIIFSILTIKFHNNKYLTFINKRITMIVNIGAIIIMIVTLIYSSFFLYDVLASNGLKQINDNLDVFISNQCINIKGILIALDQIHQYSFKIYNSNLSFIYAAFIGSIIYLIVQSLLYIIQYISSNTQVICQNPWSSRINYEIRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VDN3</accession>
    <name>A0A8S1VDN3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Calcium-dependent protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0610169</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VDN3"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000737">
        <property type="entry name" value="Protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000631">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000660">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="9"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="291"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="327"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="446"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="482"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8174565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="564" mass="66443" checksum="13FB7BBB8FC891C1" modified="2022-10-12" version="1">MKQIDRYQYNPKDMLGMGTTGTVVKAIDAKKNQYAIRIIKKNSITDDEGLQSALLDEMKLMQQLKHPQIVQLVEVIQTDNNYYVVMELCKQDLQTYLQQVHKLPESECIKLLIDILRGFTELISHGIIHRGLKLTNIMLTDKGYKLADCGLTKCMENFKKDQLLKDQDFKFHSPQVLQNSKYTSKCDIWSIGMILYNALYGYTPWSGAKIEQLLHNIEHKWLDFPDDQTSVSDEMKQFIEGCLTIEEANRMNWDEIFKNKLVKDYFKNYQTHIKEEQIKFLMNELRQKIVKQNLNISYLFQEFDQNGDQTLQFSELVQLLHTIDKNLSRQDCQVIFKKLDLDGDKSVSLEEFEKWITDNNTKMAVISKVKGRQNQRNSATKQITSLKNIQNNPVKHKDINPQGLTITQQSILNQSQSTPHLNQMPIQAYMFDPDKIIQKLQLTIQRYNINVHDLFSKFDQDFDSLLSFQEFAQLLIKIQRNAQPQEFQAVFKIFDLNDDNFISFIEFRQILNLYQQNNQKLDSYFFNTGYQYSQYNNQISPFQQMGTMSYNIYQKQNKTYPDML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VE93</accession>
    <name>A0A8S1VE93_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0620053</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8174901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VE93"/>
    <dbReference type="OrthoDB" id="317561at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8174901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="130" mass="15986" checksum="62E7BB447084869F" modified="2022-10-12" version="1">MQYSNSKQQCSTDMKQRLLNILSEKNKKVIAQQKQYSNLDQYSLQMKLSQRSHKLPIEMTKFEREQWLFEMLNNQDLEQKKVKEQGLKDIQVKTTKLITRYQYKEIKWNRKREDLQKEIDLLRLLLQQQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VEY7</accession>
    <name>A0A8S1VEY7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0610055</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VEY7"/>
    <dbReference type="OrthoDB" id="311388at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8174372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="116" mass="14031" checksum="7B2855CB1E1B39F2" modified="2022-10-12" version="1">MKCIQLDQNFRLYLGNLPDNVDKDHLHNYNKQNILELYLILHIQLYKNNCYRVKNIVLCNLEAFKKQKQHYDALVYILSEYENFKSLNVDRDTKNVCKCLFCEFETDLETQIMQMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VGK9</accession>
    <name>A0A8S1VGK9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Myb-like DNA-binding domain containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0630139</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VGK9"/>
    <dbReference type="OrthoDB" id="306004at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF271">
        <property type="entry name" value="MYB DNA BINDING PROTEIN_ TRANSCRIPTION FACTOR-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="112"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="2">
        <location>
            <begin position="140"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="174"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8175603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="303" mass="36346" checksum="EFE41FB16CE04C88" modified="2022-10-12" version="1">MNSSQSSFTDLNDWALVYQNLQTIEPEYLSLKLKLHFKTLPNFDDFIKLYESSKIGCDEVWNQNEEIFLQLVVLSLNPRCIKNRFDWEKVQKLMPKQRSLSELCFKFQSFYKAQLPRQPWSSFEDKTLLKIILDNTSRCKKKWSSIANQYNLICKSEILRNAKQCRERWNNKLDPQINREPWSKSEELHFLQLLLQNGRRWADISLKLSMITKYKRRTEFALKHKFKQLQKHYGHKAKRLNKTDFEISPRWNIQEIDMILSKIEILQEKSKKILTQEYSFCEFLSLDSEFSLVIIKNGCLIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VN49</accession>
    <name>A0A8S1VN49_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Anoctamin transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0700017</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000070">
        <property type="protein sequence ID" value="CAD8178610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="295848at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19757">
        <property type="entry name" value="Bbox1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF73">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="877"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="986"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1023"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1067"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1098"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1256"/>
            <end position="1279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1306"/>
            <end position="1327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1351"/>
            <end position="1368"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="481"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="9">
        <location>
            <begin position="868"/>
            <end position="1384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="91"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="1388"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8178610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1782" mass="211440" checksum="26DC41E601EAE6A7" modified="2022-10-12" version="1">MLQQQKSSWAQYNSPQQQRQYSSPSSPSIRSDQISRQDEIDEQWDVIVEDNFVKRVGSNQNQRFESDFTVQDQDKRNILHQTALRQDSKILELLIQDYQNLLKDNKKQERSKMQRKISQIKNNENEDEEVIEIDFDCEDYKFSEIQDQVKKYVQQPDIFGCTPIQICCFLNDSKQHQNRQQCLKILIDNGSNVNEVNPHNQWRPLHWCSFYGDEASVKLLLQNKAFSFLCDNNGLYPMDLAGKNSHTQVLEILIENMVILLKECQLFWIGELKGKNEVEYIEFQEICSKYEAELQNPLLYTKILFWCCRSKFNKFIKTILQKLPRIYIMFPIKSLDSQTCLHACCFSSNAEALKMIVKFEILDLSMMKPDPGQSKKQLKREKRREGFHFKILSNYLEYISKNDVSGINLLLERKDTSIYKERYYHELQRYLLQDQIYKYELARVNYMLLNLKDQKADKLFYDQTKQTFCRNVLNINVKDNHGNTPLHLASLNGDQNIIKFLLNKFADPEAENNEFFRAKQLTRDFTTQLYYDGLIKKQKYMISQAQLLRSIELIFQKHKQKRLKTITNFEDQYNLTENIQQKSKYYKLQLKIKIKGKQKSKKKLQTISQIDQSESAKITDLRTSNAKENSKLIQKPKKTKKVTNKVIPLNLQPQNQNKKSKSSYDHYEQLILFSSQLFVPDIVLKFDQSVKVNLIDYFQMKKSFQLGDQKKLIEQQINFQIDLLQKAEFEIYMMFSFTSEDYIYIMLRIKEEKLERLAEEMEMQIKLIDSFDLEQFKMKQRNKFEPFRSSQRQKIVYEHLLKSINIESLMKSKLVDSCYAMHTSGGIQIVKKEWQQLLFGFIPQPICQIKDYLSEGRGRNFTSLTTMRLYFGEQISFFFAWISYLTCISLIIAIPGLILQIYVILYDFHSEILPYWVLVVTIWSTVQTELWKRKTSEINTRWGCIDQMLQVESSYYEGPLKNKFSGDEEINNITKKLTKHQQITKLAAYFVFFLILLAIFLLGSFGIVYGIDILRIEVKSYKGVVFLLGALQAIAIQILNILFHYFSKWYADQENHKFELSYEKSLIYKNVFFRFINSYMPLMYIIVSSNDYNLEDIFYFLIPLVLVKKAYYILIDFLIPALIIRTKIKMYFSKVKEVSSDQLQFRKQKGKSLYFLDEIEQFWAYNEFNNNKMSQAQRSSQDNSIEVAFLESASRTVLKVESEEILEEKLQEQTQNPKLVYQPFETKIDTDAIELNSFKDEFGGTLDYFMEAVTDYGYFILFSAAFPIGPFIGIILNIVEIQMKLYKLIYMTKRIKSERMPGIGQWLNILEFLSAIGVFTNYVLLYFKQRNATLKMFTNDIDQIIQNDLELWYFISFVIAVTLIKILVRQLIPDRPEWVIEEIDKINNRELVEQQEKAKQKLRDMEKHFKELRDENTKLKKEQADLEQVTEVKIKSLDAEINKLEQQFQNISRFKKIDKLIITEYDMIVLNVLKNRYYQFDNIMLTKRLLQFLEAKSCIIQICQECGKTPAILECLECQENFCAQCYYKVHTVIQQMKHNVKLKVKKNEILEQIELNVVQDDKYQKVGVSEQSKLLKQQIGIALPKRNWKKIEYFFIPLQLQSGQPKLNELYNQFGDFYLKSSGLEFRDFKIFIEKILPVKDVLIAEDPTLRLEDKIFLNRIAFQLFRKKKHSAEIQQFLKQCTILQSGQLEQRIFMFFDFIDINEDDQISKSELEGLFLTSFVQDLRTNKRVYEMIDQFFPNNVQVRIKRDISQDFFNKVTRSDEFRTFLEALLQVQGVKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VNZ4</accession>
    <name>A0A8S1VNZ4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0650106</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000065">
        <property type="protein sequence ID" value="CAD8176486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VNZ4"/>
    <dbReference type="OrthoDB" id="278212at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.280.10:FF:000013">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003428">
        <property type="entry name" value="MAM33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10826">
        <property type="entry name" value="COMPLEMENT COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10826:SF1">
        <property type="entry name" value="COMPLEMENT COMPONENT 1 Q SUBCOMPONENT-BINDING PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02330">
        <property type="entry name" value="MAM33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8176486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="238" mass="27853" checksum="12500F79BD0DD768" modified="2022-10-12" version="1">MITKIPLRIARTLLPMNYSGLRLYISESIIEKNHLYQLIERGMIFETMVLKNTQSNSDQTEIKGFKVQDLEDDLKLELSKTVGNVQILICIECQNKEETFEEELLETQKFTVLNQTETLVQNFQTSYLNHIPFSIYLTKGNGIISCYECSAYRSKIQINMIQIIDDIEEHKQIPRQERGITDYIGRNYIIDDTLQNDMLNYLKTFGIDSELAQFVENLQSDREQALYLKWLQNKINSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VPP1</accession>
    <name>A0A8S1VPP1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0650166</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000065">
        <property type="protein sequence ID" value="CAD8176606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VPP1"/>
    <dbReference type="OrthoDB" id="300931at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="90"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8176606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="285" mass="33853" checksum="94539D18CFAD1684" modified="2022-10-12" version="1">MKSLKERMQKIDKICQKLNIPDQSKGLFKRKKNNSCIMKEQDKNINNVSMISSNAGCIISRTQLKKQQSIQMLQCDTVRSQTLSLKQLKMIHEQDQIQCMQSQKEELQNQINQLVQMKKQIQDKEDKKKKQFITQDTSLLPQMLKLQQEKLSIENEITQIDSKIEQVRYSNINKLQVLQKRNWKEEKKSFEQKWLDLEQNESKIQIDDKLYNLFIHDSPPQEQVDQKTIDTLTKEVEDLLCLKLPDEQDDQQLQQSQRSKLNQNYQSPLQIIEEEQSIELSFQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VSE4</accession>
    <name>A0A8S1VSE4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calmodulin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0710064</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000071">
        <property type="protein sequence ID" value="CAD8179075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VSE4"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000001">
        <property type="entry name" value="Calmodulin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="95"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="131"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="168"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="205"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8179075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="374" mass="43626" checksum="B84FBB6A9AD521DF" modified="2022-10-12" version="1">MRNQRKSQSPFRTNQSIMRDSPNTQLDESSISTNRIPQNRESIRARMKINQSHNNALAQGGQIRKQIANERTSAKGGNPNEFNPKKFVTKDLKESDIIDIKQVFDYYDSEQAGILSPNDLEQLLSSFGYHPTKETLYEIFSELDEDELGGITFEYFLGILNQDKSKSERKDTIRRIYRKYDKNNKGFITLQDLRQVVYKDLKEEIDEEVLAEIFKKTDSNQDGKMTFEDFYNVMTKKVAYLIIMSCFINLTQRAKRTLSIDFQFQGALPKIQLTRSRALTFNKNHISKNSFSISLKMNSISPKVNSRPVMNDFHFFKLQNCQKTQPLQELQFLKKSTNKKTIKKKPSLDFSVSGFETTDIEREDIFLQSYVRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VYM1</accession>
    <name>A0A8S1VYM1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">P-type sodium-transporting ATPase4</fullName>
            <ecNumber evidence="12">7.2.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPENT_87.1.T0780205</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>Na(+)(in) + ATP + H2O = Na(+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:14634"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.3" evidence="12"/>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VYM1"/>
    <dbReference type="OrthoDB" id="3352408at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008554">
        <property type="term" value="F:P-type sodium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02081">
        <property type="entry name" value="P-type_ATPase_Ca_PMCA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000061">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000045">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000191">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF369">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0915">Sodium</keyword>
    <keyword evidence="11" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="318"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="351"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="818"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="846"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="886"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1030"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1068"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1100"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="16">
        <location>
            <begin position="49"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00035029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00067200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8182430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1180" mass="131509" checksum="2BB6AE1D54DA69DC" modified="2022-10-12" version="1">MADDDPMTQQEQLKDDGTEPYGKFRIAKQTLCSIAYMCQERVFAEEVDKLEEIGEKLIEEGLCTDFKQGLTMQDQAERERAFGHNRKPKIEPKGYCELWLGALNDFTMKVLCVAAVISIIVDVTTADESYRQIAWIEGFAILVAVIISTNANAVNDYQKERQFQKLNEVADERKRVTVVRNGKKCDIHMSEVLVGDVVQIFEGMEIPADGFVLEASDLTSDESAMTGETDPIKKNVLSECVNRRNQLKEEGGQNTAGHHDVPSPIMMSGTRVLSGEGKMLILVVGDSSCAGKIAALLRQDEPEATPLQMKLTAIAEDIGKFGLISAILIVCVMCLRFGIERGVNDDWENYMVVTIIGYFIIGITVVVVAIPEGLPLAVTLSLAYSTKQMLQDQNLVRKMAACETMGGASMICSDKTGTLTQNKMSLVNVWNDDIIEIDTYSEKQQLTSYFPQNFQEFFIQCAVVNGSAMLRPEPKGSKTEIALLEFIERCSMNYEEQREKYPASTKFPFSSQRKRMSMVLELDGGRRRLVCKGASEMVLAACSQYHSKGNGSIVPMNQELKQKVEKSIETMAGRALRTICLAYKEISAREDLTTKDNKGVYAVEQSDLTLVAVLGIKDILRQEVPRAIQLCRRAGIKVRMVTGDNKMTARAIAKECGIITPGDDQSIVMEGPDFIAKIGGVVCTKCRTAICPCARDSTTAKKENKDVRVDTIANPQEFDKIYPHLDVLARSRPEDKYALVTGLIERGHVVAVTGDGTNDAPALKKADVGFAMGIAGTEVAREAAAIILLDDNFNSIVKAVMWGRNVYDNIKKFLRFQLTANLVSVGLTLIGAAVLSQEILKPIQLLWVNLIMDTLGSLALATEPPTEKLLYRKPHDRNEYIISKKMFKFIVGTAIIQIAVVLIVVFAGDSFLPEYPDEYDLTAFRGIKKRYKYSDHLCKTTYDDLKKYSDESGRPLTLIPDITESRDVKKDGTCLSSECVCHEKKCYEIMCEECDFETSCTIIASGRLNTPEGGRDYAVFFEDSHEPSRHFTYVFNVFIMLQLFNFLNSRRFNDEINIFEGLSKHSAFMIIVPVIFCIQILMVTFGSKAIGLYGNFGLKIQQWLIGIGFGCISIIGCFFLKFIKEDMCCECGSKEINPMETEHKIIALKGDHSSGGLARRYSSLRHDQNPFGAAHQQHQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VYV5</accession>
    <name>A0A8S1VYV5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP-dependent RNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0720005</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000072">
        <property type="protein sequence ID" value="CAD8179386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VYV5"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000390">
        <property type="term" value="P:spliceosomal complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002840">
        <property type="entry name" value="Pre-mRNA splicing factor ATP-dependent RNA helicase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF85">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="3">
        <location>
            <begin position="19"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="4">
        <location>
            <begin position="205"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8179386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="609" mass="70640" checksum="A41DA98D6BFD2305" modified="2022-10-12" version="1">MLNEFRMQLPIFQFREKIIKSIRENQVIIIAGETGCGKTTQIPQYIYENDPNVKIAVTQPRRVAAMTLAERCSLEKQTKLGQLIGYNVRFDDCTSKDTQITFLTDGMLIREFIIDQQLKRYDVIIIDEAHERTVQSDLLLGLLKNLCRKRKQLKVIMMSATMQIEKFANYLETEAIHIIEARTHTVDVYNVPIRQQDYVESMVNTILQLHFTQPEGDILAFLTGQEDIEDVKEILLERMKISNQEKQLDVKMLYSALPPEVQLEAFQKSIHRKVVLATNIAETSITIDGIVYVVDCGYVKIRSFQIGKAIDTLLLAPVSKAQAEQRAGRAGRQRQGQCYRLYTQQTYERLAKYMLPEILRVNLLSVILQMKAIGIQNVLTFDLIDRPDMELMLANLNQLVKLKALDSEFNLTQHGKNMSSLPLEPQFSHFLIKAYECGIYDLALNSISILQVENLFYFQRGAKESLQKILSKFKIANSDHLTKANILRKYEETQNKKSFCKENNLNHKTLQKAISVKQQLKDYMKRITKKEFEKEDYDKFKQVLSEALMFKHAVYSPSDQAYKLKQTNQLAYIHPESVLFNQKPKYVIYNEVILTKKVYLRDVTEIDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VZ14</accession>
    <name>A0A8S1VZ14_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Saccharopine dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0760094</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000076">
        <property type="protein sequence ID" value="CAD8181365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VZ14"/>
    <dbReference type="OrthoDB" id="10059875at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004753">
        <property type="term" value="F:saccharopine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051168">
        <property type="entry name" value="AASS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032095">
        <property type="entry name" value="Sacchrp_dh-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133:SF22">
        <property type="entry name" value="ALPHA-AMINOADIPIC SEMIALDEHYDE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16653">
        <property type="entry name" value="Sacchrp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Saccharopine dehydrogenase NADP binding" evidence="2">
        <location>
            <begin position="16"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Saccharopine dehydrogenase-like C-terminal" evidence="3">
        <location>
            <begin position="135"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8181365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="492" mass="56162" checksum="396EF56847C75504" modified="2022-10-12" version="1">MLSLYRNQLIGQQKRILLIGSGLMAEAVIDQLLKRNDNFVVVASAHVEDARKVIQNKERCSAHHLDVTETEELRKFVKNSDIVIAYIPPQFIVPIAKICAEVGKSMITSQYTFPEIRALEEECKKKGIIILNEIGLDPGIDHLATVKVRDEVYAKGGKIIEYESWCGGVPSPEFCDNPFGYKFSWSPFAAIRNINNDAKYLENGVQKYIPASELLYSTEIIHVNPSLQMEGYPNRDSLPYQELYGLKDCQKLVRGTLRYQGHCVLMAAMKSLGFASEEIIKVDREMSWFEYLLSNIRFESCSTNYLANHHITQLANTIDQKVFTLAQLETLLTKVFNRVFSQYYYKDKSEEQLYKDAEQITYTLKWMGIFDPKNLILNNVTHVHNFAAHLQTLMNYRQGETDLVAMQHIFKIVYPNDPRVYVKKSTMVKIGHRNGKSAMAITVGVPTAVATQLILDGVIKVTGVHMPNISEINTPLYEELKKEGIYCEEEDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W0S6</accession>
    <name>A0A8S1W0S6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0770109</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000077">
        <property type="protein sequence ID" value="CAD8181862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W0S6"/>
    <dbReference type="OrthoDB" id="10404569at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8181862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="100" mass="11866" checksum="35230B9EEF8A67B0" modified="2022-10-12" version="1">MGSKPTVPESTLNFLFTQSIAVDEDMANFTISLLNVYKLSILMQRLQVNRHQMFLGISKQKLQKMVKNRLFIQKRMEMAFSMKNHVNQYQINQRIMLRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W0T1</accession>
    <name>A0A8S1W0T1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase C1A papain C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0780149</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W0T1"/>
    <dbReference type="OrthoDB" id="190265at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000117">
        <property type="entry name" value="Probable papain cysteine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="2">
        <location>
            <begin position="17"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="2">
        <location>
            <begin position="276"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8182347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="512" mass="58117" checksum="4491D88BFAFDADCE" modified="2022-10-12" version="1">MIKLFLLGIVICNAFKISENFSWADVDGKNFLTYTRNQNSPQFCNGGWAFAVTGALSDRIKIKRNAAFPEIVLSTQVLISCDTQSDGCSSGTALNAYQYIKDNWIPDETCTNYVARKEECNEMAICKNCLEGMGCWAQHDYYIYTISSYGTLYGQIDIMQEVVKNGPVSCQHGQNNHYVEVVGWRTSGQSSYWIVKNTLGPKYPSLYEVPIEEIQECSYGEVIDTWTEGIRAKTQSNKKIEKRSQQKRKSYQLDMLQFLQLDQLIITPFSSLNADVPTQFDWRNVDGVNYLTNIRNQHIPVYCGSCWAHAVTSTISDRINIKLGNKYPVIILSVQSMLNCMSGGSCGGGLAQPTFKHAHLNGFTEEHCHTYEAINGKRVRCSDEDQCHQCNEDGCEPVKHAKRYFVSEFGYVKTARDMKIEIFNRGPIVCGVYATQELDDYEGGYIFSQKTNKTILNHFVSVVGWGIEDGVEYWIVRNSWGTYWGEMGFAKIKMHSDNLLLEHYCSWGVPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W1T0</accession>
    <name>A0A8S1W1T0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Deoxynucleoside kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0780018</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W1T0"/>
    <dbReference type="OrthoDB" id="567086at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019136">
        <property type="term" value="F:deoxynucleoside kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01673">
        <property type="entry name" value="dNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003847">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002624">
        <property type="entry name" value="DCK/DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050566">
        <property type="entry name" value="Deoxyribonucleoside_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031314">
        <property type="entry name" value="DNK_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10513:SF35">
        <property type="entry name" value="DEOXYADENOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10513">
        <property type="entry name" value="DEOXYNUCLEOSIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01712">
        <property type="entry name" value="dNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000705">
        <property type="entry name" value="DNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Deoxynucleoside kinase" evidence="1">
        <location>
            <begin position="9"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8182089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="232" mass="27507" checksum="DD9D24D2A3081950" modified="2022-10-12" version="1">MKTNQYTLISIEGNIGSGKSTLLKLIQQKYPDLRFIPEPVNEWQCINGDPSMNLLGSFYEDPTRWAYTMQVYAFYSRLKHWKEVLSDPINPEEKHLILSERSIEADKEIFAVNGHKNGLINNLEFALYEKFYDWLCEEVFGKKIQKQMIIYLQVDPEVCHLRMQKRARDEEKNTISKEYLTQIHNRHEEWLLRETHKNTSILVLNGDKEFESDLNQQQKMFNSIDNFLKGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W8E0</accession>
    <name>A0A8S1W8E0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Inosine/uridine-preferring nucleoside hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0840159</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8185093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W8E0"/>
    <dbReference type="OrthoDB" id="432381at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008477">
        <property type="term" value="F:purine nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006152">
        <property type="term" value="P:purine nucleoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.245.10:FF:000018">
        <property type="entry name" value="Inosine-uridine preferring nucleoside hydrolase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001910">
        <property type="entry name" value="Inosine/uridine_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023186">
        <property type="entry name" value="IUNH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12304">
        <property type="entry name" value="INOSINE-URIDINE PREFERRING NUCLEOSIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12304:SF4">
        <property type="entry name" value="URIDINE NUCLEOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01156">
        <property type="entry name" value="IU_nuc_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inosine/uridine-preferring nucleoside hydrolase" evidence="1">
        <location>
            <begin position="8"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8185093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="311" mass="35143" checksum="66A30BC155D6119D" modified="2022-10-12" version="1">MEAEKINIWLDCDVGNDDAMAVILSLFHPKSNLLGISTCFGNTSLENCTNNTIRLLSSVGRTDVPVYKGSEFSLKSTRATTKMHGTQGLYAVDKLISSFKPIEDIDLYDLMKQILGDKQFVIVITGPQTNIAKLLRDHEEIIPQIQEIVFMGGTSGFGNVTPNSEYNIYSDPEAAQFVIDTCKQHSLKLTCQLLEPIQQRIKNINTNFSDWCLEMLKEYQAAYKAQEFDYPPIHDPVAVFYVLHPELYVIKPFFCAVDCESKLCYGRTVIDKKGIYGLEPTVQFARKVVVDEFWNQMIEAIKIAAKNSKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W8N0</accession>
    <name>A0A8S1W8N0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0850243</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W8N0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8185731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="96" mass="10802" checksum="9DF003E809B59C8C" modified="2022-10-12" version="1">MNYNKALDNGSGLPDEISDLQIISLFIIILNSMINCLSLEGLSVELIIPNFLESISIAPGFDYQGQFTVKELEKINKSQFDINWMVFNTKDSINTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W8U4</accession>
    <name>A0A8S1W8U4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0850039</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W8U4"/>
    <dbReference type="OrthoDB" id="1711136at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045194">
        <property type="entry name" value="MGRN1/RNF157-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22996">
        <property type="entry name" value="MAHOGUNIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22996:SF0">
        <property type="entry name" value="RE60872P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="253"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="291"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="331"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="409"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="4">
        <location>
            <begin position="523"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="376"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="489"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="388"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="489"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8185347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="594" mass="70667" checksum="A2A962C5241EF05A" modified="2022-10-12" version="1">MKEFEKLDEIKINTKNNLLKILQTSFSNNMIQMPESQMGSLRMRPSQLRIEMTDDQQLSTRRRNFMSPTALPLFRELQQQTTRQHLQQSIAPLAMKPISSQSQITKSLLFNLFLQGFFINYYGYLFWQYSFRYVLLQLWIQDLLTMFILIYFNYRKGEDCIWQLIEAMFLFFLKVFIVFYYEIQKFKIYFITIMMISLSILVLFMRVVQKLKFQLQNNQQIIIQFIKTLLCVQMTLIALKWEDKIEWVWSQIFIILWVFLVVLALLLFISFIGCIETFINLLKKQTSQNYLIGNIWIFINIIGYTLLPFSFLYRLTQLYDNEEPFGEDETIQLIVLNGIYMLLLTVYTTFFVNNIRMFLKELQGFEQYSTGMPQQPIQIQQEQEQRNNDNGPDSNETLSKKKRKQPFRFIKLNAPLYLVKMSCTFFAIFDKIHFNQTNQKQTEQLSQRALGQQSSNRMEFQKEIKANSNSLDEKINNLEVQEQQKEIIEEQPKENEQSKKCSEIPQKSQNSINEDQQSTQDKCLICYENQPNILFIPCRHGGICEKCAEDIVLKTNQCYLCRKNIKQILKINTEGGQLIVNEISNIKQRRQIQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WB15</accession>
    <name>A0A8S1WB15_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0840157</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8185089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WB15"/>
    <dbReference type="OrthoDB" id="431948at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071044">
        <property type="term" value="P:histone mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071040">
        <property type="term" value="P:nuclear polyadenylation-dependent antisense transcript catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071039">
        <property type="term" value="P:nuclear polyadenylation-dependent CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071036">
        <property type="term" value="P:nuclear polyadenylation-dependent snoRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071037">
        <property type="term" value="P:nuclear polyadenylation-dependent snRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06142">
        <property type="entry name" value="RNaseD_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045092">
        <property type="entry name" value="Rrp6-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12124:SF47">
        <property type="entry name" value="EXOSOME COMPONENT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12124">
        <property type="entry name" value="POLYMYOSITIS/SCLERODERMA AUTOANTIGEN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00474">
        <property type="entry name" value="35EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3'-5' exonuclease" evidence="2">
        <location>
            <begin position="41"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="716"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="717"/>
            <end position="730"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8185089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="736" mass="87412" checksum="C478F5B928C7940F" modified="2022-10-12" version="1">MQEQLENIHVIQKIYSAQGNTYEEVISNFHNLQKQHLTKLFKYISNENELNQFQLKGSVIGVDIEHTNEIGFDGQISIVQIKDDEDVYIFDVIEIGLENQKLINVLKLIFEDDKIIKVFYAGSTDVLWLKRDFQIYIQNYFDLKEIADMCKLKGGNSLIYLWKQYCDHQVSKSYKNNMQTSNWAERPLTQEQLIYAAYDCYYLPYLRYVLLQELTKIDIKQQIKIFLKFNKTVNKDYKKPILSNSELAKVIQKEYNLLMQIVTETNDTSPIYQLLQTQPAAVQLVQFIALEIMKVRQEYAVHHNDPVESLEENEQVILIAIHIYQRLNIPNIQDYKLTYPDYQNYQNQQYQFTLEYISKQIDKDWEVYFKSFCKGLYQSFLYDINFIKYGLTTIFESLQVLQIPESKNKQNQQKIDQKKQKQSEFVEKFTRKKPAYGNCKIYTKENFFLCNCDEKKIRWYLKQGLADLICEDPPTIQLKFDPSGFKEEGSDKAQYVTIERVNQCVICGKENELLKFHIVPVVYKKHIQGKRSYDVVCLCLACHHKASALNDKLKIIIAEKYNINVKQNKKDSLLLMVKNLIKSVMAYNKKKQNLPKEAYQKFQIQFNNQIEQLKNQQLFNQINQFDLNNLKYEDNLININDESFSGFVVYLKLNEELFNKNEDNPDQGKLIVEQLKTEEEIDEFLVFWRDNFLNEMKPQYLNEAWAIDHKFKRTFGDKSQYNPNNQLQQKQTDDQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WD26</accession>
    <name>A0A8S1WD26_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0880042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8186891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WD26"/>
    <dbReference type="OrthoDB" id="315691at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8186891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="446" mass="53090" checksum="776BF4944ECE0D54" modified="2022-10-12" version="1">MTFQLKLPLIKRRTEQEQTNIDFKQASNKSQDVVNIQETIRSQNQRKSSSLYEISNQGSSRTTDRTKSIENSLVGEASIKQEKSNYVMKEQQKIQGQIDMRKGFAGRYDRNGEWEHYLPPIPSVKERENKQLQQLVKFTIKFVDKVPKLKLRQRLKSCFLAIKALVRWAKLVQSLIQKKRLNSFCYNNCLKEVIYNDQIKAIKFKQWTQMVFSKVFNQIKKERLDQYQNLNIIDGHCQQLSPQENDMNILKLRHLIMLMLEGLLIMTNKSNLLKELIGEMYLSQFEDQRNSFYLFTSIRTLYLQNKRDELPQMQRAMIYIEFLIMKIIIQNIQILINQDKILNTENNTLSFLLKLLISFLHYLYLNLFSKLEIIKQRIKKQLIQKQLIINQSIQLIDTPSSRDCQDLIIGIYNYESLKPILESQRWKLQMKSIFMKVIQNLEQQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WH09</accession>
    <name>A0A8S1WH09_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">H-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0920016</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000092">
        <property type="protein sequence ID" value="CAD8188643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WH09"/>
    <dbReference type="OrthoDB" id="306321at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019019">
        <property type="entry name" value="H-type_lectin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09458">
        <property type="entry name" value="H_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035805800" description="H-type lectin domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="H-type lectin" evidence="2">
        <location>
            <begin position="42"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8188643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="421" mass="48005" checksum="BACBFB1951C49B46" modified="2022-10-12" version="1">MKFILILFLKALGQIKYDSGQNTDFYSASQPSFACKNSYQLSKTINFNGAFQNIPQVFIQQSLSDIEPGNVEFRLSITSVTLTNFIVQIQCPSSKRIYSLQFRWFAIDDQRVQVISAFNIDNPTMSKTYQHLNANAENGIIFLTSISIIGPIDFVLSLSSITKTSVTVDISNPSGKFVNLKQIGYQIVLGTSEIFNMLGIQTVTTTSYTSQNFNLQTNKWILTPIVGFQFDNNQYLQFRWTYTYTASTVSCKVDSWDKLVKHTHLTALMSYVINTLYQPLQTQTIRISQKIDLEALNRPTVYAELPQTNEVFNQIGTFTTIIDKSISPVLINVIIKCSFAKKIKSQFNKCNNCSFQKFYLYSHNCNNQINQISYFSRYLPTNSAHQELTIIISDKRIQINQITYNQVAAVNNILDIEQLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WKC3</accession>
    <name>A0A8S1WKC3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0900144</name>
        <name evidence="2" type="ORF">PPENT_87.1.T0930088</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8187987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000093">
        <property type="protein sequence ID" value="CAD8189210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WKC3"/>
    <dbReference type="OrthoDB" id="282249at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8187987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8189210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="256" mass="30342" checksum="E4874671E47156D2" modified="2022-10-12" version="1">MEIIIHTFERFFGPVDWKRLKTDVLCQSLVEEGFSPKEAKELSWLYGHRNIGRNFGFVLGVGVAYCCNSWITRWTYNIPYLRLHRSSLWVSRALVVFGTLQLFDYYSTARRKGGDSGLGHDLMWVNTRYTESKERFIRNFEVLNRKLSDIEKEILTARQIQLTSPIKDRRWVYNPYIHGSDEEAFWSNVRRNFLDKLPPWERYDVQQQIAADNKEKIAEGEQIRLKPYKLTDDVDTSGVKFGFRRNPFVNNWTPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WSB0</accession>
    <name>A0A8S1WSB0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1020057</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000102">
        <property type="protein sequence ID" value="CAD8192593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WSB0"/>
    <dbReference type="OrthoDB" id="306716at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8192593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="288" mass="33850" checksum="0DF8B0B393E447F6" modified="2022-10-12" version="1">MILQANFHYEKDNKEKLASTSCKNGNDLYTNLLKLMDDIKYKIIYLDNTFHKVFAISKVELIKLPDKQDDLENFIDVVFHTSCQKGINAIAFHLRILEITNQAITIYKSEKILQILQEVYSQKGQDQQDKQEQQSNCYALRDLDYQNMIKKLNNTLYGKENFQDLTKEQQQEQEQIFSLIIQRPNSNRNTLCIFENNNEIVKVLSNFQIDKAQLYETNLAWAGEKREKAKPKLFLNAQVIESKKMKNGFTFETTIKVDHNQKSQNHLIFVLNQKVLAFQFPLKFGDNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WU19</accession>
    <name>A0A8S1WU19_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1010032</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000101">
        <property type="protein sequence ID" value="CAD8192221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WU19"/>
    <dbReference type="OrthoDB" id="293424at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="214"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="253"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="326"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="9"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8192221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="358" mass="42621" checksum="4C1FBF3684EE2667" modified="2022-10-12" version="1">MKQKLQKIKKMNCSVCLSDDCQLFTNKICKCQFCYDCVLNWIEEKINLKQQVNSISCLNHKCKQIITLENFRQSITNKQIQEKYMEIMLKNYFQQTQDIRPCPNNKCNYYAFLPTNRCGKEFSCQQCNFNWLDKQYLSYDKLFLLKIKDTKSDILSSVFEFISTQECPKCDSKILKNGGCSHMTCKKCNFYYCWRCNNQLNDNVHNEFLCFIRVLCYLLMILFNFAVTFYYLGWLQYFIMIICYPLYGLGLSLLYNSYLILPTTIIATLVYTYKQWPKLSPVRKQRFRKSQIISIIAQITISIILAIATYYNIFNITLYQALSYHYYQFIIIISGGSVVLVILLFNSFFYQNWLKFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WZ35</accession>
    <name>A0A8S1WZ35_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T1040071</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WZ35"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08530">
        <property type="entry name" value="STKc_CNK2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000097">
        <property type="entry name" value="Probable serine/threonine-protein kinase nek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000957">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF6">
        <property type="entry name" value="SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="6"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="468"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="475"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8193409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="487" mass="56688" checksum="782EAD59B7B5C5D0" modified="2022-10-12" version="1">MSLKDFQILQELGEGAYSKVYKIKRIADQQEYALKKVKLQSLSDKEKQNALNEVRILASVRHANVIQYKEAFLEEQSQSLCIVMEYADDGDLFQKIIESQKKGVLMSEKDIWNILIQIVKGLKALHDMKIYHRDLKSANVFMNSDGTVKLGDMNVSKVAKKILLYTQTGTPYYASPEVWKDQPYDSKSDIWSLGCVLYEMTTLKPPFRAEDMSGLYKKVVKGFYPKIPTIYSQDLSNVIRALLQVQPHLRPSCDKILQFQAIVNRLDDKILIEEEGAKFLLQTIRVPRNMHYLTDRLPKPNYNPIRMTKVDKYQFIQTLAVQKQNQENFEENHSMNFDFLPRLNNKKIDDSYENSVISKNNKKVDDSQLEMYQNNSNKNNQVAAIYNPPVKKVGRISNKQSKVDPLKQPKKLDIEGESKPLIIKSTKHGTEERTPLLPLLPSIKKEPQKLEEEQQLKRIKQTEELIQQLKDRQQKQRRKQKLRNLDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X5E9</accession>
    <name>A0A8S1X5E9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1110105</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000111">
        <property type="protein sequence ID" value="CAD8196070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X5E9"/>
    <dbReference type="OrthoDB" id="309806at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="29"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8196070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="425" mass="50392" checksum="F10A621D22D3159E" modified="2022-10-12" version="1">MDSSTYKQKESISKLSNFDNSDNQYNKNLVMLTTQVERLTLALKKKTNEVESLQNQIDTQKSTQYPQEMEELITTVEEQTKEIEKWAQKYQTLQQQYQQLQQQTQQLQQKNQELEQVVQLLKYPSQQEIKSTESLQSKRFYQSEISRKENKCEDDENKFISQSKLLSKQDEINQLKLQLGQLKQELLEEKQIHRELLEAEEARVRDQERKNLNQYKLETKEQLLLYKNQIQALQDMLDNKQSVQKQDNFEEKNRELNLQILDKNKKIELLENLITQKDSQINLLQQQLEKYQKEQVQLIKQQQSQKQLTLPSSSSTYRIVSTQNSSLDNNYTSIINLNKTNSIQDQINQTMAQINKSIQQVDQMKQTNSINQKMFQDQFNNNNVQSSLRISQTPQMQQNLSIQDKIKALTSLTQQQNSQNYNKYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X8P5</accession>
    <name>A0A8S1X8P5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1150071</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000115">
        <property type="protein sequence ID" value="CAD8197368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X8P5"/>
    <dbReference type="OrthoDB" id="305356at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="84"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="135"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="458"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="84"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="97"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8197368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="522" mass="61688" checksum="E096B8D80599DA2A" modified="2022-10-12" version="1">MKADSFHSLFSDKSAKYNQFISSQLFVGYKVRGKDQQKKQYIISSPSDDQKMSNNFTASQSYVNAMKALQDKIKQLELENQSLQNLSSNDSKSNLKSNERKLKESSVHMKSSENLQIKELEQKFMQQEFISKMRIEDYECKITQLQQNLLSITQQADQKYREYMEEIQNLTQQLRIQEENNNNYRNKVNSQNQIIQQEKQNYSNIQFLLEQEQNEKRFLYQNNEKLQYELSNMKEKLFEADTYIEQYINCYNDSKFRKLEEDNNQLKLQLDHLTTENQQLNESIQNLKSNLNLKTKELEESEFRRVKKSSESNIRIEQLRQQLITLSTKSNLTTISPRVTKKKNIKQVSSKEMSVKPSKIRDLRQIKSKSLNKLMDPQFLTQSQSMQKNNFMHQKQQGYDSQSSGVSRLIVKTLEENYPSSQKNEICNNAVIIGDEITYTDQQSGRKNQKYIEAVQRIRFLDELLTALNLQYQDIEEQIQQQTDMTIKKEKRQKLLEIIDSISDVNKELNDLVTIRKLASQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X9Z4</accession>
    <name>A0A8S1X9Z4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1160169</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000116">
        <property type="protein sequence ID" value="CAD8197900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X9Z4"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051746">
        <property type="entry name" value="Kelch_domain_containing_8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46260">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46260:SF3">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="143"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8197900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="554" mass="63798" checksum="1FD5EAEA14301EEC" modified="2022-10-12" version="1">MFNIQNSPNPSEIGQSQTFQLQAPCMTHQGQSVTNFCKQPDCLMPLCPKCIPQHLDKHRQQGQFADLITIDEMHEETLQLINTQLTNLNSAIQLLQQFKDSKTDAHGKLHTKLSNSKLRVQQMVDQFFEELFNELDQVTQTDKTHLDVELQQMERSLNQKIQEYQQIDQKLRTPKYLKQIIQLQTTNFLQQTQQQRVECEAFIEEVSKHTLDIQIDEQELYFLKIQLAQYASIKNAEYYKNQQIQSSSSIPKNTVYNHPYLASELQTAKVEQSPYKRLEQQLQQVGISNFTINLPDFYDPLCNQKYLHYFTDKSIFILNLEWVQNPRWQEIRLNCQVPIKSTSVRSQQGDIFVIGGYIGNQLSSQIFRIDFKQQTLISTDQLTYSRHSSAGVFLQNKLYVLGGQNQSEDLTSIESLELQLNKSRSVLETRVTLPKMNKGGPNLSICTFRQYIIKATPLEIFDTKLLKWISLNHIINKGVGMVAINNSNVLIFGGGQYQLLKMVDQQNLSFVIENLPNSPQFGQIQNGSLIHQGKIYAVTSTCNFIIGTPDGWTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XAE0</accession>
    <name>A0A8S1XAE0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TmcB/TmcC TPR repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1170038</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000117">
        <property type="protein sequence ID" value="CAD8197989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="312072at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22249">
        <property type="entry name" value="UDM1_RNF168_RNF169-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057352">
        <property type="entry name" value="TPR_TmcB/C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25474">
        <property type="entry name" value="TPR_TmcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="70"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="105"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="222"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="280"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="931"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1132"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1318"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1533"/>
            <end position="1553"/>
        </location>
    </feature>
    <feature type="domain" description="TmcB/TmcC TPR repeats" evidence="3">
        <location>
            <begin position="450"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1259"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1259"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1276"/>
            <end position="1285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8197989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1594" mass="188086" checksum="C2E1104DBD6B22B1" modified="2022-10-12" version="1">MKETMSQFICSGLVYKLFVFVELMQNIYFVMHPILQFELDIVFVQYFRQVIQYSQLTYILDAMDQNQSIILLYTAFGIQITLLFLIGLGVNFVKQKKKITTITSYSFKAISFYALILNTFLHVCFINIFLVFLICYPNSQYSQDVECYSGLYILHTVMAALGLFLYFIIQYYFSTFYIDLKPFSDVPFAQPQSRYDYMKILFKCAILLFITIDYKAENPQTYIIFVLILYFILLMFRIYEPQHFQLDIYNFLIACDVIQFSLAFSYTLHKFLDNGAPDTVGFYFILASIPFIYYCAIQIGHKKNHFFQVKGIKKLSDPNQHEQLFNQLIFIIDRRDNLQNYLLLEGILVQHMETCQNSNCQCQYLSESKSKELDDLIWYKFIIQMIDLSITKFPKSCKLHLLQSYIYNNKLNNKFKCYYSLQHIKSLETTLKEEFQSYRDLHTLEEIMQEEDQKAEIIIDVLQVVKFQKYFGQFQNLLEESVKRHYEFWCELQENNPDIQKLFTLGSQITQSVEDVKELYDKLIEINPNHINTLRIFGQFQNEVINADNLGLRILEKATQIENAQLAYSNETQTEKYGENSNTCIMTCSAETKSLGLIQSCNKEIEKLGYHKYELINQFITRLMPKCYAENHDKFMSKYLETNESKIVGKERTVYCQHKNGSIVPCLLMFKILPQLKKGLRMVSFFKVLENYDSHYYILFNPTTFLIYGLSQNCQDSFGIPANFSYGNNAHSNDFTMEILILDFNHLVEKLKISQENIQTAFPLQLDTTKLQKQFIMEDSVDRLSLMEEGEINNNNNLLNQNGTSEQLKSSVKKESLFKIYKISCTVEKQQYTDLEVAILKFTEATAFQEASKSGNYYQSEIKLKSKQTKYSSSATKKKADQQINEDYLNEDRSSVNSVQSSLNDDMRRLKDFKALMSEKKVPRNIRTIQITMLSLTLILLTVSIVDLVLKGIQNTSFKYSSQILSDVYWRTNQMSTITFHSRILEMLGKGEYNNSDGFTAQSLRYDLLSRLKIVQQLQFDTLKVKNDLLKQNVEVPIQYNYNFTSLLIDNSFQETELTLTDSIREALSHLVILIGTDLQNFKIGDYITPAQQSFFFINYNGLRNIRLGLEERAEQFYTFFQDLSSKKFGTFLALMIISIIILIITHAIVIPILLKVDRANNKVLSMFGLIPPIEIKELSDKCEKFLKEYIEDFNEKKELENRINNKNQDENKKQQQQDKHYIEMNNEEHIDEKQKEQMEKEKELQRLEDEEKERLRLEKMEEDKRKKQEEENKQKRYLRHKKKKKHKYNIQEIELTEDERTRIEKLERSNEKNDSSALIKFMLFFILFTGFFVAQLVLEVTYLNSITFLFEHLKLSHQRYYIMMYRVVYGIEDIIQEKQINIDNVWMQQLYHTQIYDAERDVFESYKSFPLTGFDTYKQYYDWFNFNDMCSNITLMSSLGVTITESGCRQVQNGILEKGLRTSVINLALYTNDSLKITGNNTKYTIINGNTFQIINDIVKYIRPAFNTLNDVYIADSLEYINYAQNIEVVKFIVLIIAWIILFFIIWMPYLTKLSIQIWMTKGMLNMIPMSIIQKNEKLKYRFLQDNIMTMVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XB50</accession>
    <name>A0A8S1XB50_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1180032</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000118">
        <property type="protein sequence ID" value="CAD8198248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XB50"/>
    <dbReference type="OrthoDB" id="299709at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="104"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8198248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="220" mass="26272" checksum="8F04B6232AC7E3AF" modified="2022-10-12" version="1">MNEQKLREMMKIYTVSKQVNKKAEKNFIKFSSQYYKLHKNDMTPDLLNEVNSLRTQIKSDRVAFSNQNSPQVSPRLTTNPLFNFKEEKPKLNSIYSDDKHQDIYKLYIEEISDLKSQLNQANSIIKQQKIDYDRKILELNLKLESERARIKDLNQQIQEMKSNQLTKSIVPMLNYSFDQKKQKQNSDLNKEQSRELFKTEICKALEKYNNNKQDQSKEYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XD35</accession>
    <name>A0A8S1XD35_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0960053</name>
        <name evidence="4" type="ORF">PPENT_87.1.T1200048</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000096">
        <property type="protein sequence ID" value="CAD8190309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000120">
        <property type="protein sequence ID" value="CAD8198875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XD35"/>
    <dbReference type="OrthoDB" id="6508832at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23807">
        <property type="entry name" value="UEV_UBE2V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000026">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="2">
        <location>
            <begin position="8"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8190309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8198875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="140" mass="15622" checksum="8C6A45279B0367E9" modified="2022-10-12" version="1">MQGAPEVPRRFRLLEELQNGEKGIGDGTVSYGLEDAEDMQMTNWNGTILGPYNTAFENRIYSLKITCGPQYPKVAPQVRFVTKINLPCVQPNGQIDINKFSILKNWVSKSTLESILTGLKNEMSSAANKKLQQPPDGQTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XDG3</accession>
    <name>A0A8S1XDG3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">H(+)-exporting diphosphatase</fullName>
            <ecNumber evidence="2">7.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T1200062</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.3.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000120">
        <property type="protein sequence ID" value="CAD8198903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XDG3"/>
    <dbReference type="OrthoDB" id="5210at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009678">
        <property type="term" value="F:diphosphate hydrolysis-driven proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01129">
        <property type="entry name" value="PPase_energized_pump"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004131">
        <property type="entry name" value="PPase-energised_H-pump"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001960">
        <property type="entry name" value="PRK00733.3-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01104">
        <property type="entry name" value="V_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31998">
        <property type="entry name" value="K(+)-INSENSITIVE PYROPHOSPHATE-ENERGIZED PROTON PUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03030">
        <property type="entry name" value="H_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001265">
        <property type="entry name" value="H+-PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="74"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="97"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="143"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="289"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="330"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="370"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="416"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="447"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="552"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="624"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="718"/>
            <end position="741"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8198903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="744" mass="78970" checksum="F77FB65CA8A9D777" modified="2022-10-12" version="1">MEFVTALVFIFIACACGIVWAIFNWMAVHKLEIHHKHEGLTELLQGAEQEKIETLLEIGEHIQDGAQAFLKEEYTDCSVFLAIMAVLLIFISPWSSLAFVLGAATSMLCGYLGMAIATAANFRTAFSAITSLSNAFQMAYRGGCVMGFLLVSISLSILTLIIIIYNAIIVRSDDNNFEDLVTMFDYVAAYGLGGSTFALFGRVGGGIYTKAADVGADLVGKVEKNLPEDSPKNPATIADNVGDNVGDIAGMGADLFGSFAESTCAALVVSSTQLRVVQGDTYVIEIGQLMYPLMVSAFGIGICILVSAYAVYISKVNHINKIESTLKFQLLLSTVALSPIIIGIAYWCLPADFVMVAADNSIQLSELKPWHAFLCSLMGLWSGLLIGYFTEYMTSHSYTPVREVAKACGTGAATNIIYGLALGYLSTIVPIVAIAITALLSMKMLSFYGVALAALGMLSNLTIGLAIDAYGPISDNAGGIAEMSELGENVRESTDALDAAGNTTAAIGKGFAIGSAALVSLSLYGGYLTRIQTYKVGDQFPFAEGAKIDDPIIFAMLLIGAMLPYAFSAFTMKSVGKAALQMVEEVRRQLHEHPGIYAGTEEPDFRACIAISTKASLKEMIPPGLLVIVTPTAVGLFFGPYAVAGLLPGALISGVQMAISASNTGGAWDNAKKYIEAGFYRNEAGEVKKKGSDEHKAAVIGDTVGDPLKDTSGPSLNILIKLMAILSLVLAGAFCRTGWLYKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XDV6</accession>
    <name>A0A8S1XDV6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1210071</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000121">
        <property type="protein sequence ID" value="CAD8199234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XDV6"/>
    <dbReference type="OrthoDB" id="304976at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="613"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="652"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="139"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="237"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="393"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="522"/>
            <end position="613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8199234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="731" mass="87178" checksum="4F9BE87085745422" modified="2022-10-12" version="1">MNRAKIEETNRRYQEYKAELRIPILPSSQQDNYYAQSLHKLYPEITPSHVIKPHQAQAFNPEKTRLYIESLQKQNQEINCELRQQAHDIICLTQMVNTLIDENRSLSLLLEQKDQEMHSIVETMTINEAEEISDLRHQLRLLEDENSVLLKHVKEQRQQLDEKIEDAQELEKQFQNSKKYCLQLEQQIEKYKLSEISLSEQMQILKENCKTEIEMKSSYQFENQKKDSVITSLTFTVEKLNKQLLQLSSDYEKLESDKNELHSISNLKIYELNQKLDDLSNELSKYQITNDTLIQEIIQQHKEITDKQQLCERLADQNDDIMNKLSMEIVKQKKLYDNENRLQEHIGQLSIQNIEYFNTIKYQSEQINTLINQSEEDLNTQKQRFDQVILKLKQDYVNELKEKQLEIKELEEKNTSILKEWNNVKNEYEILKIDYEQNQKEISDYQQQKRIIEQLQLQNRQLKLQLEENKALLSEFVSHKDGVQADIEERDKHIQKIINQKDDRIAELERDFQIYYDKCAILQQHQKNNEHLEQLNFTYREQKELFEQDNERLKQEIQKLQGQINNYATVQKQQKIKAEKQISEENQLLRKQYNEIANELQKLKDQQANMNTLPIQPKQPSEAGSRVRQGGQSQDFQQTKQNQVYLQPQQLIQPPSPHHSQHSPVLSRRNIQESDDGSVKGGGNTQEKKMQFSFTDDLMKQQQQVKRQRDRENSYHSNMSNAPYRKKSQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XFG9</accession>
    <name>A0A8S1XFG9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1220064</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000122">
        <property type="protein sequence ID" value="CAD8199621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XFG9"/>
    <dbReference type="OrthoDB" id="301578at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8199621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="312" mass="37026" checksum="A074833B1F7066F5" modified="2022-10-12" version="1">MVKQQKKSNFTVIPPQQDFQEQEIELLQEDLNDDLIKTLSKANTNSIATIKEKEKKVVEFKSRVLELVEIFVQNTKNPTIFIERIYPFIKMISDFKNPQKIQYINRVCKLIHQIIQKGDLGEHIDNMKQCCETLITYLHKCQDKSINKRFFELLDLILKQISQHDKKAVSDIIVKGLVLMRKNSNIQMQNIRKLIFNYQFTLIPVILKLCQLSEINENYERSKYNFSHLLFTLIQNEQIKKKIISKDKIKQAIVKLAQHTLENYAQQNYKRVAIFRQILLSYKSLELNELQEQIKTFQSEDQNIQHIITKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XFU6</accession>
    <name>A0A8S1XFU6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1220176</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000122">
        <property type="protein sequence ID" value="CAD8199843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XFU6"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8199843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="58" mass="7108" checksum="3691FF5E5418F3F6" modified="2022-10-12" version="1">MYQERNWKNIRKFKREKLDRKQKILQAIIEIKAFQILPVENEKTIVKQGIVEIKQLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XH44</accession>
    <name>A0A8S1XH44_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1230148</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000123">
        <property type="protein sequence ID" value="CAD8200149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XH44"/>
    <dbReference type="OrthoDB" id="319737at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8200149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="201" mass="23255" checksum="0AA60D7CB1328AD0" modified="2022-10-12" version="1">MGNQIEIGLINRVDNRSGVKTGIWEIYWIYVEKNNKIAGGEYDKQGNKIGKWIEQSDRFRYGNQSTSEGKYNKQGVRVGMWDIYLKNDGKNNKIGGGEYDEQGRKIGKWIEQSNEFQSVNQTTWIGEYNNKGVKTGIWDIYQNNKGNNNKIGGGRYDEHGNKIGLWIEQNDGFWTDNQSTYTGEYNVWGSQRWKMGNILEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XIU2</accession>
    <name>A0A8S1XIU2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1250071</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000125">
        <property type="protein sequence ID" value="CAD8200668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XIU2"/>
    <dbReference type="OrthoDB" id="430293at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="1">
        <location>
            <begin position="46"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8200668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="171" mass="19816" checksum="41ED00E9C7FC7807" modified="2022-10-12" version="1">MSNQNQQFALLQSSQQPLIDEQIAELPILSFLNKRRPIKAKAQLTVENNDFEIEIINTKVVQNSFVVYEIKIKKGTLFWIFQTRYSLLESLSSKLSKNLKSNLPKFPEKRLFGNLDNDFIVQRGKQLDSYLKALFKLGRSENTVREFVRQTQKAAFMINDPTAVKLFNLDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XJI7</accession>
    <name>A0A8S1XJI7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Band 7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1250053</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000125">
        <property type="protein sequence ID" value="CAD8200632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XJI7"/>
    <dbReference type="OrthoDB" id="190994at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043202">
        <property type="entry name" value="Band-7_stomatin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264:SF19">
        <property type="entry name" value="AT06885P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264">
        <property type="entry name" value="BAND 7 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="Band 7" evidence="2">
        <location>
            <begin position="45"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8200632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="306" mass="35142" checksum="A1A4D668D7673391" modified="2022-10-12" version="1">MQQQIIIGSIVCFASFLLIILIIGWDVVEVTNWGLKCNSISKQCDKQIYAPGRYLVGPFNSFFNFPGSRLTIEFSDDKRAQSQPLKTRTAEGLTLSLHVSFQYQLIKNEIAQLYALGGLNYEATFIRMARDTILQAAGKFEAPKYWTNRRNITEVMQNQLQDELKKAHANCVSLQILDIELPDQYEDSIVQTQIEVQKKTMKQFEQKAQMILNDILVMRAENDQEIYAIHAQAEADAFTITQAAQATANKLLLEAESKGYEMIQKNLELSLEEFNQYLYWISVLKQKKAKLVFNPNTVLTYNMNRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XKU5</accession>
    <name>A0A8S1XKU5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1290029</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000129">
        <property type="protein sequence ID" value="CAD8201795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XKU5"/>
    <dbReference type="OrthoDB" id="294878at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8201795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="550" mass="65031" checksum="03F37EC3912ABED8" modified="2022-10-12" version="1">MDELLSAVMQRKTKINEVLETIQKLKVKNVISQSERSTIYAIIRKLKEEEKNRTEKENWIYNEEQMNHIFVKTNDIMKLINESFIELPYQPPNSLIQFLEQYYNLHQGFFQQMNQEYQLKIQYKQAIKQGNINLITDLIKALLASSRKILKDNIDDLAYQVNKDLSPEKQKIMKKLIPDPLVEHLRTRSSTQNGKKDGTVISMTNSVIQNEEPIRYNDKKEYQQIKEKLKQQQRRFILQPKIPNQEIMLLESPVYQLKLKYQQYNNINIEKNQFASLFNNVNLDDLDDQNICVLDYQTIKRQQQNFEEFKLNFDSLYDKIKMRNSYIQQSVMDYNQYISKKCINSTMMLSSQCNNNLENEFFNVIKDKYEELTSLNAEFQQNMCHYHFCRIYLKPHINPICPCYNTNTSKYELNSNPIKNKIVIRKSVQSIKKSTPIQQNQNTFTSKNLIDQLKKERTFNQSIILEDSSYQDIYKYNNSSGISPLNNSILQIRKLDQSQNLNGSTQYKLYSHKLSSDITSFKQTSLHHRAQSANGDQLIIPPLNLRNCAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XN56</accession>
    <name>A0A8S1XN56_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1320064</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000132">
        <property type="protein sequence ID" value="CAD8202826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XN56"/>
    <dbReference type="OrthoDB" id="2096344at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044458">
        <property type="term" value="P:motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052803">
        <property type="entry name" value="Cilium-Associated_Jouberin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44499">
        <property type="entry name" value="JOUBERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44499:SF1">
        <property type="entry name" value="JOUBERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="475"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="850"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="850"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="864"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="970"/>
            <end position="979"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8202826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="979" mass="112534" checksum="98601187719B8209" modified="2022-10-12" version="1">MSRSNIPRQLPPLQQKRVGEGEMPFGGQQQISQPAEKKVDFANSNFEQNDEQQDNEEEKLQPAPNHDSGMTYTNFMNDDIIYNCYSLGDRIGARINRLLQCDPPRLTFVDRLNKLEYDQIVNPFSLSEKDKAQITPDRTVVVKIIGTSQLQMDTNIQHPFIRMHVINIKNGCYLYKPKSEDNQPREFTNAVYNYESNCITNILDGERERVTCELELLTAFSTNFCDMRMATNSRAQWNEEILINIPADEFYKSDTIILFELLDFHPIYLQQHNSEFLDSDNFYRIAWGYTRPNGLCRLHLGISKIQLYKYLFDTNKLKSVSRRESIPLVYFDFLWLDKVEYDAVLNVYLGTEPKPQFIEIYGKSHSNNVFEIEKGDEEAREKVKHSVVMDKIIEQRIEEITDQEKINLRRRRYLGDKEDHIPDKIAYKFASAPMGCYRLAFNKYGNYLACACTYKNSKTIIKLFDVETGQHFTTYKGHRNIIHDLQFSQNSQYLITVSSDYTAKVWSVPQHSGDIVSEEESDLLLICTLQHASFVYAGLFLMDSKMPVVATCCFDSRMRIWQFESNQQLQIIDEPIQQEIISHDGISFDQFENLGNHRHPNCLVYDGAKCLYVGDSLGNIHFFDINLSGGSYIARRLSLIVIQEISGNPINQIQLMPPECQDLLIHTRDNCIRIVDFKSGDGKFHDHEVINGSVNNRFFGSQSVKIACRSVSSPKGDYVLSGSEDGKPHLWSNLTQTLPTDIFQFNVIGPVADVAWNIGYHMIAIAGFGDEHPILVYVWEREGDLNFIEAKKLQDEIKIKHREQEDIVFNIQTKQTLSRFQQSIQAQGLFQPQFAQSSQIPFQPQSIFNQQQPANQSKANTQTGPFGQSGPFGQSQQQQGPFGQPQQQQQQGPFGQSQQQGPFGQQQQQQQQQQQQQQQQGPFGQQQIPYGQQQPSQNSGPFEQSQQQGPFGQMPFQQQQVPPNNPNTPYNANYQMPFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XNJ5</accession>
    <name>A0A8S1XNJ5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T1310111</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000131">
        <property type="protein sequence ID" value="CAD8202583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XNJ5"/>
    <dbReference type="OrthoDB" id="286238at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15505">
        <property type="entry name" value="PHD_ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000750">
        <property type="entry name" value="Chromatin modification-related protein YNG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028651">
        <property type="entry name" value="ING_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024610">
        <property type="entry name" value="ING_N_histone-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333:SF42">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01408">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="10">
        <location>
            <begin position="162"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="7">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="7">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="7">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="7">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8202583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="214" mass="25120" checksum="6669B18934C9EBFA" modified="2022-10-12" version="1">MSNIEEFIDLISYAPIDVRRAFSKVRELANLEKKYEETLESTQNLVFKVKSVDSSGENGQFILQLKEQIQRNHLQLLALHRERIAALEIIQQNYQKYIATLDIEGEKFLEELKQLPREQEDISRKSQRDVKVEPPVMVQKSDKIKKTKRPLQDEQVEEAQQHTYCVCLQPSYGTMVACDSKNCQIEWFHLSCVGLTDVPDEKTKWFCPHCRMMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XPT2</accession>
    <name>A0A8S1XPT2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1330093</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000133">
        <property type="protein sequence ID" value="CAD8203229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XPT2"/>
    <dbReference type="OrthoDB" id="286020at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8203229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="92" mass="11089" checksum="4595AEE67040E984" modified="2022-10-12" version="1">MEKWKQTIYQPPSLFLERKRTALYRRLDPLLVAPLTKFDHKAYDQLFKSQANQHLRVRNLSLQSDRVQELPKQLGLEDIVFKLQKIRKLRNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XSI1</accession>
    <name>A0A8S1XSI1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1340145</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000134">
        <property type="protein sequence ID" value="CAD8203728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XSI1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8203728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="216" mass="25398" checksum="3853EA83683AD04E" modified="2022-10-12" version="1">MIAQMVLANFKIKNLTLYAIIKWRINKDTIPLLSDDGLIFRYEIVSSLEQKFFHGVKLKNIWFILKIQNNMELWNHLQQNQKESINILKDIELYDQCALCEKQLNKEDKQFINHYGGEGLCMSFSNEQLEQQGEEIDKAKLINDLTQSTTQSVIANYHNKAKLYFVRNFTYPFGHKVGKIREYSNVCHLVILIEDWILYNCQICDSVQYCNTCGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XY00</accession>
    <name>A0A8S1XY00_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1430120</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XY00"/>
    <dbReference type="OrthoDB" id="307589at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035907171" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="358"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8206280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="516" mass="61129" checksum="61F47052E1FCC302" modified="2022-10-12" version="1">MLIILTSIILLLSLITKQISQILQESSEIIFDLYSKRQQDIAKQTLEIFWVTYIHSVSQPLISLHRLYNFQQRLNNLKVNSQEDNENCYNQPLIEIDKPCICYLNKSSIDSNQNDPKNMLKNLMQFQTALPTHIFGVQQYMGFFEESQEQNLFNYPCLDFRDYKLYDPKTRPWFIEAEIAYNQSNFQQYSVRLTGHYLTIPYNDFRFSIALPLINFQKNMMGTLRVHIQSKILKDTIYKNSNKTNLILTTNDGMLLISSLFNNSGFELGTDYFYNETKTGFNLEDWQAITQKNLSNCEHVDIGFTCRQNKIDKKDYYMLHTTLEQYNLKIILYQTKYEYQQYLQEHQKQMIIQIQEGLIKGLVFLIVIAIVISLISSLFLGKMLKPLQQIAKIFQITKHFSSQSITQRFLKFKISNNYAGIFKKPGCFISKDIFLFCQCIEQFVINFSTVKKKSNSIYERFDQIKYPQKDEKDQINFLDYFQQQLLNNKFQELDINQKQIRNLIKTCLVSSTRKEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XZM4</accession>
    <name>A0A8S1XZM4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1450110</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000145">
        <property type="protein sequence ID" value="CAD8206896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XZM4"/>
    <dbReference type="OrthoDB" id="299726at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12431:SF14">
        <property type="entry name" value="LD15323P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12431">
        <property type="entry name" value="SORTING NEXIN 17 AND 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="2">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8206896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="210" mass="25113" checksum="990F6774D43BE0E4" modified="2022-10-12" version="1">MQDNEIDILIQVPEFKKNEDQVQYRVHFLNQKSNTEFENNYRYSELKRFHQTLETLKTELPTFPKSYWWKSVNSNFQLIEQRRQLLDSYFQNLIQIKLVRESLIYKNFVLVALKENNKKVQKENKEKRQRSENQIRKNTKQLGQFITPIPNPVNLPPTPEDKRSQSMEHIPKTCQQRNERKKSSKQQQSPILNYGGSKIFRGVLSNAAKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y0N9</accession>
    <name>A0A8S1Y0N9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1400034</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000140">
        <property type="protein sequence ID" value="CAD8205204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y0N9"/>
    <dbReference type="OrthoDB" id="304580at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8205204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="183" mass="21373" checksum="ED7DC64E612AEC20" modified="2022-10-12" version="1">MLITTKEHYVMIKSAKMRTDRETKQSTQANTSSTNKSYYQDERSRLQQLIIMSMQNPKNMETYLTQFLDQKHPISNSFQQNRTRVLSQPHLGNKPTRFQKPLHNPYISRRQSHIPKVDTSPKSPTRILSTNSRYLLYSFNGDCEKKRTKNTPISKLSTSALIDYHKQLVLKAQKLIGSNKKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y2A9</accession>
    <name>A0A8S1Y2A9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1490131</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000149">
        <property type="protein sequence ID" value="CAD8208091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y2A9"/>
    <dbReference type="OrthoDB" id="1278353at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000465">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001155">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010926">
        <property type="entry name" value="Myosin_TH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06017">
        <property type="entry name" value="Myosin_TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51757">
        <property type="entry name" value="TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TH1" evidence="7">
        <location>
            <begin position="3"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="211"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8208091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="511" mass="60418" checksum="269DDDDB0E995A5C" modified="2022-10-12" version="1">MHQDNRKMNILKQIKIETNDIYLLDAEKYFKKQCTSLEIILISRMITKINKRNNEQLRMIAISNEFVYNIDKKSIKRKIAINKIFGVTISKKSSEFIIHVPTESDLLYKSQENRDIIIFYLAIALKMHNIDGIKIYFIEDEILQPYCMHHQTLDKSKQTSLHPKCQITIIYPENFQLNYINTMNKSKTTSNDLFVLFVNDPLRLRVKLDEYQKSVVLAQGSLSKIYLLHNKYQKQQQFSVLKCVPINSVDIDLLELFLKNYEKLPFIEELELCMLYNGNVNLIFQFVKGGDLYQHLKDINNFQEQQVKQIVAQIALALSDLHERGIIFGDLKPENILIDERGYVCLTDFGYGKLRMYQEYKKTQAINFTIEYSSPEYIKNGELTRMSDWYSLGILIYELLVGITPFYHNNFEISLKLILKGEIHFPKNIVCSSQCKDIIQKLIKHDSTQRLGFLMDFKELQSHPWFHDISWEDLAQKKYDTLAYIPQFNEISQISDRYFTMEQLNEDERTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y6W3</accession>
    <name>A0A8S1Y6W3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1530031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000153">
        <property type="protein sequence ID" value="CAD8209033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y6W3"/>
    <dbReference type="OrthoDB" id="293109at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="127"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8209033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="272" mass="31680" checksum="B0C69778FEAB974E" modified="2022-10-12" version="1">MDKLDKIIAQLDELEISQKHREELLKRNQATFLLKLPKVILIQRVCGFLDDNDLYRFTATCSTLRKIMFCPLGFKLLMLSRNANHLVGGPKHQEIQLKHEKIDIITCGNLSTNNNIFETEEDALAQLEVLKAVKDFLKSKLQDAQITIKKLEDMLEDAQATLKYEKSVNLKLQSKINILQNQLSISELQRQDVKENLAELNSKYNKIITQMEQDRIILLEDKEKLMGHKKVLIEEVYRLRGLVSQMEENQNNYREALRQVKTFMESVEVKPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YBK9</accession>
    <name>A0A8S1YBK9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1600012</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000160">
        <property type="protein sequence ID" value="CAD8210588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YBK9"/>
    <dbReference type="OrthoDB" id="285119at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035739632" evidence="1">
        <location>
            <begin position="19"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8210588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="275" mass="30999" checksum="4C6BAC8C3446E0F8" modified="2022-10-12" version="1">MHSIQIILIISTASLIKSTTLDLNNRCKCSELIYSSDCYNGFSDCSWNTHQNQCVDVACSDIEWSNHCIWSSNRCYWLNKQCHDFTSCEAIPGKDQSECIAANVYCPASNGINCLSMQYQQKCSDIKDPDTCNDYYSPQGKCMWKDQNCIILQSCNELWTNKTKSCLLNACYFDAQSFMCKDMTCSYYTMESQCQFGAPTPGPYLNNLIPCEWNTQTGQCQEANPVDLNANNCYSNSAMTYHWSSSKSSKGGCVSCKSSILSIFIGLMMMIIMIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YEC6</accession>
    <name>A0A8S1YEC6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1560061</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000156">
        <property type="protein sequence ID" value="CAD8209834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="310950at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035822100" evidence="3">
        <location>
            <begin position="24"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="225"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="319"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="496"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="637"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="683"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="816"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1048"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="225"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="267"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="319"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="371"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="414"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="503"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="637"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="691"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="832"/>
            <end position="849"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8209834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1454" mass="172175" checksum="939A3FB3BF4CF857" modified="2022-10-12" version="1">MHTLRTSLLLCMLIYINYAGSEALSLLNQMQPKFNEETDLGSEDLIYKKATEQLKQQKPYLQIDEEDTKNEVSFREFYKSVRSGKHEIVQDEDEINMVEPEHKEIEIQSYLQELSDGLEPQTENIAETELQTDPEKLGKFKKYFIKEEEPKVEVKIETKSENNQPIEKKMTLREKLEMKKKQAQQPSVVAAIQIKQEVNNQEQLTNQTDQNVGKTTLMKKIEEFKKQKQSKEIQEIQETQQSKEEIVQEEQNTKPVQENALRKLLNKKAEEKKEKEAQQQIQKSVEERQDTLLGLTQEAEIEIKQIDVVNEQDIDFLNEKESLESENDLKQSQDETNLQELEQEHDESHQEYIISDENISNNDENTEQQKEQVQSQSEIQQQTQIDNESSSFESDQSDSIVQEQDQNEQEIENVEQINPSPQNLEQENDNNNNNNNNNNNNNNNNNNNNNNNNNNINNNEQEQEENSQITKLIDDDQINQEIQQDDQELQQFEVKQPDDQQQELDNQSQNEQQSEQDNQDQDEKQIIDQSNQEINIYDQMENTLSQGFVMENNVETSNYLEEVQEAEKLRKKKEVEEMIQEEERIKQELEQKKLEDELKLNELEEEQKQLNDEKQKEEQQIEEVKKQIELEQSELKDFEQEKDVQSQIKDKDINDIQEVQPQQEILQEDLNIQQDNLNQQNVEEQTTIQQDKIEPKEIKLEDHTIQSQDEALDNGFNIDEYERLQHRNEDKTEQNDSNYSQSSELFHQNDYTSQILNDQQEQVIEQSVPTLSINEQQTEEISYEPQIQISKDVTIDEISNEQVLINQEQDGLIDQNYIPELPNPENSSIQEQDIKQKLQEEENEVKEQEQQQLQADNEINQDNIENLINNDSSQEELKIESYNENENIDNSQNQVYTQQQENSNIESDTPNLIPVDDSQSNIYQYEQEAENEDNEVIQFDVYHSSSDVNTQNNEQQIINEINIESQPIVNQQEVEENTNVLSANKIHSFSLLQQDMKIDDTIKIEYIDLEKKQDQSTLNHQKRQQNSLLQVEETEMDYNEEQFEIFMAQKKQQEQMEKEKKRKKLKAREEKQRKLEEEQYQQKLLQQKQQQEILKQQQQLIELQNQQLLNKKKYKMSTKQNNQVNVDSIPIKGDNYIQLQKQQSQIQILNQQIDTEFSFQQILSKVEKEQRQGQKQLRSNSKHVKNTDLNFFQLTEKQQQNKISLQSQDLFEAIKQEIKECDLNNYTINLCQLCQSDVVYEINDDNWYLIGIAYGLKRHFQNLLNTVQYHFKNAKIYTSFNKEQQVCLTLGDQFEIENLEEILNYYNNQTLPKNTQFLKIPQKIRSNFAQMKVDLNKHKFMNFSLNAAIKNQEHHNGFTVTEQQIKFSQDFDYIQQGSILYPGQSIPYKLNVQSNGIENMQLDLNPQIYEKILENYLAKNYLQQIQITYRDILKSNFVVIDNDELLELEITIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YFX1</accession>
    <name>A0A8S1YFX1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1590027</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000159">
        <property type="protein sequence ID" value="CAD8210374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YFX1"/>
    <dbReference type="OrthoDB" id="10352539at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8210374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="190" mass="22221" checksum="EF992E44AA63538C" modified="2022-10-12" version="1">MSHDPQIDLLNIEQTKREFSQIPIKNQLLYKFETEFIAEPWDAPIIPVKQKNQNTQFISFKQPKSHIQPRQLNTEIPLTPQRPISCQQKNQFVDLGILVSTPTSTKIIQRIIISAPRKSSRKNSNIQYRQKFIQSMLVTQKQNSLLQNLMYSSSDYCNFISQINPVSQKNKINKEPQFTKCKFKLKTLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YL12</accession>
    <name>A0A8S1YL12_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1110159</name>
        <name evidence="3" type="ORF">PPENT_87.1.T1740034</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000111">
        <property type="protein sequence ID" value="CAD8196157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000174">
        <property type="protein sequence ID" value="CAD8213147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YL12"/>
    <dbReference type="OrthoDB" id="756370at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="448"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="493"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8196157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8213147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="711" mass="85635" checksum="68635B8117B40896" modified="2022-10-12" version="1">MKHFQPDSCLQHNKKAIFLNQSEVDPQFKNLCLQCVSELKNELIVIDDFQQSIQELIINLIKQREQLNQNNLQCLIHLKDCILQFKMQQIISIEKYIQCIDQQINIMDEFQKQIYDYQIPIDNVDQFLSKYTEIEQLHINYEGIRDVIMSNLNSIQIQDFMPQFQEIINNLIFESQTDVVVTLSHIKSHIFPKNYDKEKLKIFCEKHNRQILMLDLNKQKTLQNRLACIDCIEEYAIKFTKLENFEQQWKDYQNTIQQENNKFNERITNTNKTILQYMNDFYKLLQNELKYTQKQLTDHANDYRMKTRQPIFKTIFQFSKEEIFEIAEELSNQNNQEKLTKEFESQYHNFKIQSDKILEDQEIIIQQLYKKMQKQIQESYYNIIDQHNINQQKIKPFKYEIMKQNCIKQQESCYALAFNNDSSILIAGCNTKIKIYQFINEELKEKQIISEQKEFTCLIFMKKRNHFISGSKDGSIIIWSIDNNQQWYYQQRLEEHNEKILCLILNECEDLLISSSWDKTIKFWNNTTKWISTQTILDHNEQVWSISMSPSQTKLISCGNDRIILILEHKKKENFWIIVQKINVEVRGYRICFLSDNSFVFQQWISNLMNFYQYDETKNEFKKKRDIIVKSGNSCDYLSPLQYIDNKSLLVNKNGFHINMIRRNEEETLKTEFTINFDTNYIYISISRDGEFLATWDNKTQEIQIRKFLID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RW51</accession>
    <name>A0A8S1RW51_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0010171</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RW51"/>
    <dbReference type="OrthoDB" id="427480at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13969">
        <property type="entry name" value="ADCK1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045307">
        <property type="entry name" value="ADCK1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051130">
        <property type="entry name" value="Mito_struct-func_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173:SF28">
        <property type="entry name" value="AARF DOMAIN CONTAINING KINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173">
        <property type="entry name" value="ABC1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="146"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8131573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="486" mass="56839" checksum="4C1AF07D11E9EE41" modified="2022-10-12" version="1">MLRKAILLGAFTTGCTFFMPPQQKKDLQGFTAGFINSFSAIKTLIESLYDYQVELTKYEYNTEEYHQQRSIIHQRVADRILRLSIENKGVYLKAGQYIGNLERVMPREFTQTLRVLQDQGPQVSFEDVKIVLEYEFQKPIQELFTTFSHKAIAAASLAQVHQATYDGKDVAVKVQFPQLRVQYRYDLMIIHNIAKLCDFVVSNTSTSQLNFSDLFSTFRKALEKELDFTLEVQNAEITRNNFKNNSRIYIPQFYQYSQRVIIMEFIDGVKINDVNKLKNPRECANILIDMFGQMIFKHGHVHCDAHPGNILIREQNGKQQLVLLDHGFYTDIDQEMLQNFRCLWNNIAKFNYKQVEQYALKLGIKKDHIEFLPLIFFYRTISSKKKLGDAFSIEERQYLRNKDLVTLENINALLRSMPPEIMFIIRAANLVGIHNALLGGTTRDRLLKFTEYSVKNSEKSVWKQKFEWIKLKIALFLFEFFGLIPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RXG9</accession>
    <name>A0A8S1RXG9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0020081</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8132808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RXG9"/>
    <dbReference type="OrthoDB" id="668540at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010608">
        <property type="term" value="P:post-transcriptional regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF12">
        <property type="entry name" value="MATERNAL PROTEIN PUMILIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PUM-HD" evidence="3">
        <location>
            <begin position="161"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="185"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="221"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="257"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="295"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="331"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="400"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="443"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8132808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="514" mass="59423" checksum="1DC56CDB6273F8C9" modified="2022-10-12" version="1">MSAKVYEAEDDLLGSILKNDDYEEVFSNNNSPLRKQSFESDSVIENVSTSEKHYFDSFNRSFEQISLGSDLAQSPMSNRALYPYCPLNHFKFSRQDDRQSVSISFDGPSFSTQESSINYSKIGNGYQYKQNNRFVDYQVPYSPGLLRPQGIYQPGFNYQQLQNGISFTGYQQERQPLHEINDDIQLTNDLDNMCGNQVLSRKVQKIFEHGKPDQRELIFNKVERSCVQFSKDVFGNYVMQKIFEKGTPIQQQRLFNKIRPHAYELSKNNFGCRVIQKIIEIISNNESLQNQFIDSIRLQVKSLLYDSSGNYVILKCLEVLQKEKIEFFLQPIEDSTLFLCSSQYGCKILLKALELFSPIQTDKIITTCLQHQQNLCEQEFGNHILQFAIKNSNYQPLVINFVLSNFEKLCMNKYASNTVEKVVEASTNELKQHIISILISKSQINGLATFVNLSINPFGNYVIKKMIIMSNHNILQPLIDILRQDQYLITAIRSSDFGQRIYQLIEKTLLMQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RYV2</accession>
    <name>A0A8S1RYV2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Anaphase-promoting complex subunit 4-like WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0010678</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RYV2"/>
    <dbReference type="OrthoDB" id="439331at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024977">
        <property type="entry name" value="Apc4-like_WD40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47822">
        <property type="entry name" value="CARBOHYDRATE BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47822:SF2">
        <property type="entry name" value="F-BOX AND WD-40 DOMAIN PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12894">
        <property type="entry name" value="ANAPC4_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Anaphase-promoting complex subunit 4-like WD40" evidence="3">
        <location>
            <begin position="55"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="194"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8132570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="375" mass="43501" checksum="2A61C42CF21412AA" modified="2022-10-12" version="1">MRPKPKNNKEQQNVEEQQPQHVIKEEKPISDKYQVKYNSKTQLRLDEKESFVLGVPGTKIYSVKWDADDKYIACACENGTVRIFNVRKRQLSYLINSLMPNIPFSYVKWRPQAQQFKTRNIFVTGNTKGEVQHWHMTSGKCLGTMKEDNSDIYCIDYNQDATKLAVCGLNPIIRIHDEEKRVVDVRLGVEQTQPPGHNNRVYCVKFHPQNPNMLISGGWDYRVLIWDIRQKKPEANQIYGPLICGDGIDMRESFDGQQLLTASWSQEKQLQTWDLRTCKLICNFDWNSQIKVSNQPCQLYSGQFSRQFDNQLTLAGGSGENEVRIFDSQDFDNAQICIHDLCREVNTVDWAHKDSRFAFSGGDGYLRIFEIQQIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S182</accession>
    <name>A0A8S1S182_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0030082</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8133998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S182"/>
    <dbReference type="OrthoDB" id="70770at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="22"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8133998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="356" mass="40799" checksum="47753933F7B6E22F" modified="2022-10-12" version="1">MGQTCSINTCCSKEELQTIRNSSRSIEIQSKKSKVSQQKNQEQDKVEGKIMKKKSDTVSDVMVSSQHSKISIKQIEKNLTTQNQSQQLNPLVLKTMGMKERLQLPALQLSNGATYEGEWLNGKRDGFGKQQWPDGSVYEGEWKEDRSCGKGKLIHADGDIYDGDWLEDAANGLGTYIHLNGAKYSGEWLNDYQHGKGIEIWPDGARYEGDYYYGKKQGKGKLNFADGSCYEGDFSDNEIQGFGHYLWPDGRFYVGQWSNNKMHGFGEIKWADGRRYKGQQYQDDKKHGKGTFFWDDGKKFVGTWVKGFQNGVGIFFQSDNSFRIGEWNDGKRIRWYNQNEIDELEIQGILDDLRNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S2N8</accession>
    <name>A0A8S1S2N8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0030322</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S2N8"/>
    <dbReference type="OrthoDB" id="311772at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8134468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="249" mass="28523" checksum="BD211A96F0ABBA0A" modified="2022-10-12" version="1">MLILIVLSFAYAQIQDEQQLTNAACVIFSRYYLQSNQELAGRAVGEMIQLQQLTKEDAVMFAVASLVESCLESISTQETMEIIQQLQSQKINLEAHQHLVEKADLKKYGSKKKSAKKNEILKIIKSIDDMVNQQKREHEGGDDGNDQSETGRKRQRNKGKQQELPDMSIFELKEWLVLSSFVLVGVLLSYIICCVPERKKKEKKDKTKVKSKDQNDDKLSPKKEEENSQEEDESSPKEVAENKEKKKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S3V1</accession>
    <name>A0A8S1S3V1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Profilin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0020453</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S3V1"/>
    <dbReference type="OrthoDB" id="10259541at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41752">
        <property type="entry name" value="PROFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41752:SF1">
        <property type="entry name" value="PROFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8133534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="146" mass="16611" checksum="C55EEAE57F3B3F4C" modified="2022-10-12" version="1">MDQGLDPAMLLAELKKQDEWAKAIIFDEDLNVITHKNCAATKEELTLYLKAYDVRDNTIGAGFVLLGEQYEVHRWHPPLIYGRRGDADVGEGISLARGICNKLNGKKVYLLITYELPIVSARAVPQQINFYNQFIGELEKFDIKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S5F0</accession>
    <name>A0A8S1S5F0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0030511</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S5F0"/>
    <dbReference type="OrthoDB" id="305802at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="187"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="236"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="380"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8134803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="448" mass="52570" checksum="F98BB2FC2D171789" modified="2022-10-12" version="1">MGSACTKFVCKDTLDSDLMSLESKMRTLNEMSKSTKFSSLKLSITYTNLTNQRVSETEIRDQDNPINKINKSKGRMKSFNFQEDHQQQFWLSQVSRVTDSPVTVKSETRRIKKKNNPQRELIQIQNLDLSFYIQRISYQLENNEEQSQRSILKSPLNKSFNSLSEHKKIKQIIAIKKNKTQSFEKKFNLNLLFYVCISIILYYFYQNSQMGSCQIQRVESDTISLKDSSSKRIQSKKSNNVNQNVQSSNIENKLEQLDQKVEDEDNLLTVYSRIKKDKRLYNFQEEESQRKISNRGDVESLKQASNQKRRLFRTSVTHSHFDESNQTSQPHNQSLQAEKDDDAKSVKSILKKNSKIKDSVSGSPETRSVRFARGTIFRTKSQRSLRHSKNKKHNRPDIKMNESINSFEHRSPNWKRHREQERMQIIKVPAIFSMNQNIGVNTKLFPYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SCA2</accession>
    <name>A0A8S1SCA2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tubulin gamma chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Gamma-tubulin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T0060233</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome, suggesting that it is involved in the minus-end nucleation of microtubule assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SCA2"/>
    <dbReference type="OrthoDB" id="10249382at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02188">
        <property type="entry name" value="gamma_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000004">
        <property type="entry name" value="Tubulin gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000003">
        <property type="entry name" value="Tubulin gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000012">
        <property type="entry name" value="Tubulin gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002454">
        <property type="entry name" value="Gamma_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="12">
        <location>
            <begin position="48"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="13">
        <location>
            <begin position="249"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8137715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="759" mass="86622" checksum="39160868EFF834BF" modified="2022-10-12" version="1">MPREIITLQVGQCGNQIGIEFWKQLIKEHGINNEGILEEFALQGDDRKDVFFYQADDEHYIPRALLIDMEPRVINYIQTSQFSTLFNPENIFISKDGGGAGNNWACGYSQGERYQEELMEMIDREADGSDSLEGFLMLHSIAGGTGSGSGSYILERLNDRFPKKIIQTYSVFPNQNETSDVVVQPYNSLLTLRRLAQNADAVVVLDNTALNRIAVDRLKIPIPTVSQTNSLVAMVMAASTTTLRYPGYMNNDLVSILAGLVPTPRCHFLMTGYTPLTIDRHISSVRKTTVLDVMRRLLQTKNIMVSTSTKNGSYISILNIIQGDVDATQIHKSLQRIRERKLASFIEWGPTSIQVALSKKSPYIETSHKVSGLMLANHTSIHSLFDRILKQYHKLRNRNAFLDQYKKEPMFKDSLDEFDESKECLESLISEYKAAESKNYLDWGNDDNMRIEEQHEGVQQLTKKEEFAIDTGIKSDDLIMHSTQGKSKENKDDQYSQCSNKSALPQIISIEDELLIQSTLNSSVPLKLRKILMEDDAYYEGEWMQGKRWGQGEQRWPDGSIYIGEWKNNKANGYGVLTHSDGDVYKGDWLNDQAHGKGVYINFNQAKYEGEWVKDRQEGHGIESWPDGSIFEGNYKQGKKEGFGKLSYPDGSKYEGNFQMNNLHGQGKYIWPDGRIYEGDWINNQMNGKGIMKWEDGRQYEGEYKEGQKHGYGTLIWEDGHKYVGQWVMGKQEGVGEYFFTNGISRKGVWKEGKRIQWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SES6</accession>
    <name>A0A8S1SES6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0070069</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SES6"/>
    <dbReference type="OrthoDB" id="310099at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8138346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="222" mass="26524" checksum="22CF5D9DA9D59BF8" modified="2022-10-12" version="1">MSFPQQDYLAMLKAKTPPYPPVQQSQQTPMIYPNPQFLPPLMNYPPYYMMMQQLQQIPQMQQLPQMAHMRIPMIPQTHQLQNIHQPVMPFQMQQQNPTQQKIDHDIPVTVKGTKVKLNLKIIKDLPYEEDSILDYDEIKKQSADPKYINLKRKRRLLDQQEAPLMQTRRRSEQSQYLEIREMLKVDDKEMTIILEAYPDTKTLLQELRRGNLKREIEKLLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SF74</accession>
    <name>A0A8S1SF74_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0080301</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SF74"/>
    <dbReference type="OrthoDB" id="298185at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="144"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8139801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="202" mass="24298" checksum="42302A01FD18A41D" modified="2022-10-12" version="1">MLSERKVKLTLHLPKLESCVQFESDIYTTTSRSRILSITKAHKSEKRILHKHTVTQMNTSNQLHLYTPKYESVQAKRLKNIPYQLKYFIPNLPNKNFKSSHLMNKKLLETPQNIIQNKDKWQLNKYKTRMNNAFSLSQSKRMQNKHLKTEQQEEQNEIRRQNQNYIENEKVKRLNKQRIETQKIIDQYTSQFNSSIKLNHIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SHM8</accession>
    <name>A0A8S1SHM8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinesin-associated protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0070022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SHM8"/>
    <dbReference type="OrthoDB" id="10265679at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035869">
        <property type="term" value="C:ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016939">
        <property type="term" value="C:kinesin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044782">
        <property type="term" value="P:cilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008658">
        <property type="entry name" value="KAP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15605:SF2">
        <property type="entry name" value="KINESIN-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15605">
        <property type="entry name" value="KINESIN-ASSOCIATED PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05804">
        <property type="entry name" value="KAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01297">
        <property type="entry name" value="KAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="497"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8138252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="583" mass="68204" checksum="70DD226913630405" modified="2022-10-12" version="1">MDSEDLLEKLLEAFYDEKIEPKKEAAKKILGLAARQENLELLISHEQFLSTISRTLRDEHKKSTELTLYLLCVFYIVSNYLEFHQILSEHQIGDITMKIIESQIQRFDLRYAELMEKQGQPQQLQELRKLNLMIAKQEKLFYVGFTILMNMAEDPIIENKMRKRKIITFLLRMLERNNFYLLIVTLLFLKKLSIVNECKMQMVEENCIRKLKRFFSADNNVLLQLSLGVLKNLSFDSEARLQIEQNGFIPDIVKMLKIPNYRFVSIVLLYLLTLDDKIRLTFGFTECMTLVVKLMLHFPEPVIGKELISLAVNLSTSSRNVDHITEQEFQQIVQRAITNSDTLLFRFVKGVIENSTNPEVQTCAKGFVRHFMKLLVTQGKEEDIKFEIIGIMSALDLQENWIKLLNEPFIEFLHNNLAVGMVDDDIVLETMMLVSSIASSQNSAEKLFKSRILNALSQVYCEKGDDDEFVVQYLYCLNQFLFHKIGISQILEQDEILIQIIQQLNDKNKKVKQMSEEVLDILREYDQELCEKIKEIKFGEYNQLWIEHLNEQELMLQDGADMAFEDEYGGNELDPKMWDSDND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJE4</accession>
    <name>A0A8S1SJE4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0080406</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8140008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJE4"/>
    <dbReference type="OrthoDB" id="264354at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00060">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46210">
        <property type="entry name" value="FHA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46210:SF1">
        <property type="entry name" value="FHA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-CH-type" evidence="5">
        <location>
            <begin position="245"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="4">
        <location>
            <begin position="392"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8140008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="502" mass="58676" checksum="2DF86A2F24658F60" modified="2022-10-12" version="1">MGSCQCRKFCESEQVNSNTHLQEIATNDDYRVNNQEGAAKDLFIKIKTWQKESFSLFDYENQSHLKEQNFQISKGGYLIKNNNELQWIDDDVDWNKSVIKQEQILFRIDKNDGIFSIINKRGECKQNYEDDKQEQTTKYHGDNKDFQQSENLDQLEGDEFHQPNDIIKKYSHHQKISMSQRTNTKLMEKGSKLWLVVRSIQQMMSNNGIKLKEGDVIKMGRVKFKIREIQLNQRQLIQDSDSAKSSQSDAITCRVCCSSAFSRSNPLVNPCKCTGSIKYIHLNCLKKWLKSKFQTKQSDHCIIYMWKNLECELCKFNYPPIFKSDEGVFDLIELSKPTEYPYILMEITQKRYEEMQENNMSEDTQWNQCNGVYIITFDNNNGEVKNIRTNELKIGRANETEIRVNDISVSRNHGILKLIEGQIYLTDNKSKFGTLILIQQSVIPLIPELNGIEMQVGRSVLQFTLGNNNANQQQQQQQYLESEIFDKIIGRNLEDEELDYLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SS75</accession>
    <name>A0A8S1SS75_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Calcium-dependent protein kinase 1</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="ORF">PPENT_87.1.T0110333</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cell membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Parasitophorous vacuole membrane</location>
            <topology evidence="24">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="29">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SS75"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020002">
        <property type="term" value="C:host cell plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020005">
        <property type="term" value="C:symbiont-containing vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000398">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000632">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000557">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 26" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17" id="KW-0969">Cilium</keyword>
    <keyword evidence="15" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1032">Host cell membrane</keyword>
    <keyword evidence="16" id="KW-1043">Host membrane</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0519">Myristate</keyword>
    <keyword evidence="12 26" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0564">Palmitate</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="27">
        <location>
            <begin position="130"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="428"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="466"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="502"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="539"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00060437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00068067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="EMBL" id="CAD8142780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="568" mass="65146" checksum="E7DFB0F2A1A27530" modified="2022-10-12" version="1">MGATCCKSNLQTQDELSVQSAAFGDNEQSSQKRVVKQTTANKRQAYTPAQTFGLGLKQNLSEEGKEVYDEPKKVSMVVSKELEQLLERQNSDTDRKKILEQQSAQKKEHKLNVNIDIFVQLKKGQISDHYITGQVLGEGAFGKVWKVTHKKSKLDRAMKQLKKSSILKEDKEKLFSEMNILKNLDHPNIVKLYELFEDDKNYYLVTEYCSGGELFDRIKKMNFFSEKKAAELMRQILSAVWYCHNQKIVHRDLKPENLLFVSDSPDADLKVIDFGTSRKFETGKRMTKRLGTPYYIAPEVLLENYNEKCDVWSCGIILYILLCGYPPFSGRSESDILKRVKAAQLKFDPEDWAHISEDAQNLIKNMLNPNPAKRISSEQAYNDKWIQNNAPSNQVNQKALQNLQQFHAKSKFKQAVLTFMATQIITQQEQDELNKTFQAIDKNGDGKLSRQELIDGYTSVTNNKELAIQQVDRIMELVDINRSGEVDFTEFLIAAMNQEKFLSVQKMEQAFKIIDLDGDNYISKAELQNVMGDVDDEIWIQILKECDSDNDGKISLEEFSSLLQSKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SUS2</accession>
    <name>A0A8S1SUS2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">methionine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Methionyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPENT_87.1.T0120381</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Met) + L-methionine + ATP = L-methionyl-tRNA(Met) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SUS2"/>
    <dbReference type="OrthoDB" id="5844513at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036464">
        <property type="term" value="C:cytoplasmic ribonucleoprotein granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017102">
        <property type="term" value="C:methionyl glutamyl tRNA synthetase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07957">
        <property type="entry name" value="Anticodon_Ia_Met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00814">
        <property type="entry name" value="MetRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.20:FF:000001">
        <property type="entry name" value="Methionine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000037">
        <property type="entry name" value="Methionyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00098">
        <property type="entry name" value="Met_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041872">
        <property type="entry name" value="Anticodon_Met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023458">
        <property type="entry name" value="Met-tRNA_ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014758">
        <property type="entry name" value="Met-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033911">
        <property type="entry name" value="MetRS_core"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00398">
        <property type="entry name" value="metG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001100">
        <property type="entry name" value="PRK00133.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765">
        <property type="entry name" value="METHIONINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765:SF1">
        <property type="entry name" value="METHIONINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19303">
        <property type="entry name" value="Anticodon_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methionyl/Leucyl tRNA synthetase" evidence="14">
        <location>
            <begin position="264"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl-tRNA synthetase anticodon-binding" evidence="15">
        <location>
            <begin position="686"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="182"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="182"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="221"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF09334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF19303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8143740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="831" mass="97064" checksum="D9E161B81D57A6AF" modified="2022-10-12" version="1">MIFAFKKLSGKTLSVLKNNIQVKLDFSKCCDTLQINENESVYGNYSIINYLSKVKRNPYQNERLWWVAHQLKEVIQIKKNDVVVEKKKQVKTENIKTLNKILEQSKYLEGEELSEADIIAYIKLKRVKPEVKTQGKLLKWQNTVGALVDPILNSMENEFKPQIAAIEEEKKKKEEEIKKRIEQHQNAKKDTKGDQKKGNQKQELPQQQQQQQQQQQQQQKQQKEKGTKQKEEIPQDYLKQFSAQKIVRTNLGQQILPQKGKRNVLITSALPYVNNVPHLGNIIGCVLSADTFARYSRLRGYNTLYICGTDEYGTATEVKAIQENMTPQQICSKFFEIHKNVYDWFDIDFDYFGRTSTPVHTEITQDIFNHLHPKYTTQKTTPQTYCSQCKMYLADRYVSGTCPHCQYEDARGDQCDSCGKLLDPVELINSKCYICKNKPEIKDTKHIYINLPEIQERLETWIHSTSKTGLWSQNSYNVSKAWLKLGLKERCITRDLKWGVQVPLEEYKDKVFYVWFDAPIGYLSITANFIKDWKQWWMNPDNVELFQFMGKDNVPFHTVIFPSTLMGTGQDYTLLKTISTTEYLNYEDGKFSKSRGTGVFGDNAQNTQIPNEIFRYYLLTNRPEKSDTVFTWNDFANKNNNELLPNLGNLVNRALNFIYKNYNKAIPQVELNQLTVKDEEFINLFLNKFRTEYLEAFENVEIKEALRKAMELSSLVNKYIQDEKAWEKEQKESKRSDVILFVAANAIRFVSALIEPFMPAVSAKINFTLGFNQRTEKDDKLFEHVLSYDNNISALLNLVKPGQPINQPVPIFREFKPEEIENWRQQYKGQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SVJ3</accession>
    <name>A0A8S1SVJ3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tubulin-tyrosine ligase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0120365</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SVJ3"/>
    <dbReference type="OrthoDB" id="202825at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_125, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069">
        <property type="entry name" value="TUBULIN TYROSINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8143708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="583" mass="69019" checksum="69CED88E5B5E12EC" modified="2022-10-12" version="1">MFLSVSGSGFHMNYRQEVRLPKLQGHKVQAYSMPKYRQLNSPEDKPISPDRFLKYLNNKVQSQAGVYRNPVAVQSSMILIAKQMKFSSDQHTHRQKEKSIKIIEQSNEYKYNLGLGNNHELVQRIMDTRVDWQQTKDNSSMFVNFRWQQWNRGYKYDRLILNNQYKQMVNHFENHPELSNKQYLFRNLCSYSEQNKQNVYDSVPITFVLDFKDDSVDQQFTQFVKFFDKYAPKKIDQMAKKLVEYKKKLKIPVQPMNDKKIQPIPNKIPKTQNGNQYLWLLKPTFLNRGQGIHVVNDLDTIISHICEYQEGHQTIEDEDAKNNKKNNNIKANSFVIQKYIEQPFLINQRKFDIRVWVLVTQDLHCYFFKEGYIRTSCENYTTEDVSNQYIHLTNNAIQKYSEKYGEFEDGNQLSFDDFQNYLNKQGFNIDFQQNNVTKMKQLVWMSMQSVKRKLNLHQRKFCMEIFGYDFIIDQDFKTWLIEINTNPCIEESSGILKMLLPRMLDDAFKLTIDVIFPPKQKLEIVESTFPVNKYSNTESLWESIGTLESPKPASKQSALLLPNQLSSQIQQFQTIIPIYQGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SVX0</accession>
    <name>A0A8S1SVX0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0120278</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SVX0"/>
    <dbReference type="OrthoDB" id="302334at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="200"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="2">
        <location>
            <begin position="131"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8143536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="219" mass="25440" checksum="C652FD81BBC90464" modified="2022-10-12" version="1">MNYYTTLDGGSKLQDIIEQIRQMDINTSETLKQRNVKLQNNESIIMIDKQLTISIDGINDSIKQARILLNKSSNLSQNDILRFKNLIDEFQNNSEKIRKALEIKQVFKIDIPETKIDFTSKSNVEMVQMQKQLVSKQNETIDKMIDTTGRMQNNGKQINLALDEDKVILRRLNDNVEQTTHEIKVTDSNLKSLLSYANDCCLWTTIIIQFMIFVFLVIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T1G3</accession>
    <name>A0A8S1T1G3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0130459</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T1G3"/>
    <dbReference type="OrthoDB" id="2624652at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035935778" evidence="2">
        <location>
            <begin position="16"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="143"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8144904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="261" mass="30264" checksum="4D9A8E3268E4FA92" modified="2022-10-12" version="1">MIFLITLLYFFLTSSIDIKSIKQYKRLKRYKYFFQLLTFSVPNNHLTTLIIVKLKLNLNCNILQIINIDKSLSLSTQLFQESELYNNCYLQDDFHEINQFIYKIFLNNSQIIKNLILQLNTSTNQILNLNQFTKQLPKNYGQCFGHSNNVRSVCFSPDGNTLASGSRNNSIRLWDVQNGKEIQSADKKYKDILAKFKAPLFSNNPLPESNNITILRISQTPLFQAQGALILKGEFINYEGYDLKPLFKSKGSCFLEDLKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T3C0</accession>
    <name>A0A8S1T3C0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calmodulin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0160138</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8146960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T3C0"/>
    <dbReference type="OrthoDB" id="302610at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000527">
        <property type="entry name" value="Calmodulin-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="6"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="42"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="79"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8146960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="165" mass="18915" checksum="07371BBCCD839CEB" modified="2022-10-12" version="1">MDPAEKRMKDIKEAFDQFDTDNKGTISTKELANILKYLGQDPSDEELDNYMKELDPESTGTIDFLQMMKILTKAVKDDDTIDELMASFRVFDVDNTGTIPTAEMRYILIEMGEKMTAQDVKDILKEMDPDDSGMCKYVDYVKKKYEDLQVAKAKAAKLKAKKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T5B6</accession>
    <name>A0A8S1T5B6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0160041</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8146766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="266718at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06606">
        <property type="entry name" value="STKc_MAPKKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF56">
        <property type="entry name" value="MAPKK KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="67"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="359"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="367"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8146766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="408" mass="46688" checksum="46542D126BCF00E6" modified="2022-10-12" version="1">MGCCFTKKKNQLKPTKAYEYMNTNNQNIKQQELEQSFIQDVEVIIIDNKEEELERNSLKSDQDIHQWEQGEVLGQGAFGKVVMGLQKNGQIMAVKQVFIQNQNDDKVKQLQKEIEMLSRLQHPNIVRYIGCEQKNQFINIFLEYVSGGSVQTLLERFGCFRERLIKTYLKQILLGLSYLHAKNVIHRDIKGGNILIDNSGRCKLADFGSSKQLSDITHDTVGSICGTPNFMAPEVINQEQYGKKADIWSLGCTIIEMATGQPPYSEYKDAIAVMVRIGKSTQPPPIPSQLSSPEAKDFLSKCLQIDPKQRATADELLKHPFLEEPKSNSLLKKTTSYKFNQRQVQKYHYQNKNSFLLDAENQEQEIQPVSPQFQGQQGQSSTETNLITVKKKKEKPQYQLDKKXYKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T628</accession>
    <name>A0A8S1T628_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0170224</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T628"/>
    <dbReference type="OrthoDB" id="292444at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="122"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8147950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="637" mass="75034" checksum="0635EF23B3E2F85D" modified="2022-10-12" version="1">MSRKRIEAPPLTDRSQFRRISQKSVDELNQTLHARINSMVGLDEQLSTKIKQLQVSTFTTKDLTSIGSSLQDMVTTLQKVSFNEIMYNQLLRDNEKFKNEQSYLKAQLFKLNNLNSKFQQEHQLLLEKIRSQEVDINNLNKKLTAEQKLNEELSKKLKRSEQRVQIILESNITIQKDQLKHNLVEVLTENEHIKKDAIQKQKDFQKLQMVNNSLNQKLSRLNNRFQIRSKVQNEEDFIDQEKMVITFTEIPKNFVFRHVFQNLECKCNNLQFKLEFIQSVLEFSTEDFLIHINSVNPETKKMQLTWLFNYMINYKEFSLAHNVFIIKLSKLMQIYSYDEMHNFFTTLSSFFKVSHIYLWLRDFQTGFFINQFDGKQKKIICTKGAFRDCLDTRESVNKLTAHKHIMYQNDMGEDIYQDSTYIFPIITDAALPAGLIEFHHPIQSNNFSDIQYFASILGLYTKIQIQKIDEEIHKTSLLKQTDILQSQFLKLMKAIDKFHFCELMKEMQCKVMQLSTSEIVFVENNGLWKYHNEEIKKLDNLAGVCGQVAISKQPAYFANISKELNFNQIVDMYSIAPLFIYPIVHNNETHALIQVSLTNKQIDKYRFKDPLVSLSSQSNQARLFCDYVTTAYIHKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T6H6</accession>
    <name>A0A8S1T6H6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0160348</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T6H6"/>
    <dbReference type="OrthoDB" id="10252009at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000129">
        <property type="entry name" value="Dual specificity protein phosphatase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF511">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="5">
        <location>
            <begin position="18"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="6">
        <location>
            <begin position="79"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8147378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="327" mass="37623" checksum="75A7BD6B96EB4DBF" modified="2022-10-12" version="1">MLQPRPPFPSGQMDLILGKIENYGALFLSGIEAALNPQLMQQNQIGAVLTVGTELSNLKFDCEQKLIMLHDTAYDPIRRHFEEAIHFIDEQRKTKNVLVHCFVGVSRSATLVIAYLMQIYNYSLQAALTFLISRRPQINPNPGFMQQLQQFDFELNRRRQQRPASTQYVRCYNERDRRESMEVRSTTRQFPNQSPLKRVNQKAMSTKSLFLTPKKSEAQNYFNFTPIVKPNNSLEIKQITKSAQNLDNCVLRQSNYYGNNTKKLAETAINGFKSASSFCKTFSNHFPMSKNQMLAQTQTLLKRTQGSGLGHRGRQPIRAQTKIEYNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T6J4</accession>
    <name>A0A8S1T6J4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0160353</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T6J4"/>
    <dbReference type="OrthoDB" id="305911at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8147388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="152" mass="17848" checksum="436039E1E12DDEFE" modified="2022-10-12" version="1">MNDLFKEIDDTNSDTSIIDFNFESDDEEIIDKNDQPIQVIQDQVEIVNQEEQIILEKGSSIQGQQNCQEQNTFEQQGEQNVISLPVQHDHLKPKLIKYKKHRECNLQQGYQILKTFGYFAHTSYKNVQQKKEQAFQKITNYIISGVEKIKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T6W0</accession>
    <name>A0A8S1T6W0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0160045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8146774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T6W0"/>
    <dbReference type="OrthoDB" id="314948at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035836759" evidence="1">
        <location>
            <begin position="17"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8146774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="150" mass="16886" checksum="DFAF9F5EA90F5620" modified="2022-10-12" version="1">MLKTILFCILLLSVFAGHVRKTHKELHNKNPRKFNSAFLEFENLGQKDYHLSNKEAQHWAQITADLNSNTKKRQSLIEAHSEYIPGVVGNVADLSNNVGVYSYTVTDINGNILESETGDHLAQKLNAAYLEMTSKIQDSSSLKLQDNNQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TBU5</accession>
    <name>A0A8S1TBU5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0200010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8149940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TBU5"/>
    <dbReference type="OrthoDB" id="10354745at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8149940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="337" mass="38712" checksum="86C01499184ADEF5" modified="2022-10-12" version="1">MQNIQSSLEFFPYELIPKMTTNNRYCHAIAINHNNTIVMVEDRGNIKVFNLKNESLKLIQIIQQLGFSVSTINFFKSQQFFVSSSYMGDIIFRSSILKSTSKFLLKISENITQINCLLINSPQEDLIITGSADHAIRFYQSKSKYPLSWVCSQTLIVHLNCVQGLSLNEEGTKLISCGSDLKIIIMEGSNKQNWIVKQIIQTSWKGFRINFITNDIFIFQQGLRNELDFYMFNSQSGLYSLDKKLQVKGGQKSCYNLFPSKYVKSKQLLLVKNGEYVNFIRIINQPSSNQDLECTLVQAINFDTGLTFGTLSDDGQYLITWDYNSQQIKVRKPKRIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TD43</accession>
    <name>A0A8S1TD43_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">START domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0180272</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TD43"/>
    <dbReference type="OrthoDB" id="291971at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00177">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051213">
        <property type="entry name" value="START_lipid_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308">
        <property type="entry name" value="PHOSPHATIDYLCHOLINE TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308:SF56">
        <property type="entry name" value="START DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00234">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="START" evidence="1">
        <location>
            <begin position="86"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8148849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="271" mass="31263" checksum="7A041102528F0030" modified="2022-10-12" version="1">MEQLCLNKIIFKSKTQQIKFYTNLIGISNILFQLLYRTQYNFEVIQLQILIYHSNKLSIQQTCKQITKQSQNLQANAIELAYQTIEEGKWEKEIEQDGVVFYSKPGSTGWKITRSELIAEIDPKKAVDVLRNYTRFQEYNHTAQEINLIKKIDDNISIQYILTKPNQVLQQQRDIVTLSRASSLPDGTFFVVSKSIEVPEAPIKEQYVRAEIILSFFGFKPVENGQTLVTMVQSFDPKGDAPKDLLNSLANKVVNYNKLFVENLKKATIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TEV3</accession>
    <name>A0A8S1TEV3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fungal lipase-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0200255</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TEV3"/>
    <dbReference type="OrthoDB" id="424277at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051218">
        <property type="entry name" value="Sec_MonoDiacylglyc_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45856">
        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45856:SF25">
        <property type="entry name" value="FUNGAL LIPASE-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035933288" description="Fungal lipase-type domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="Fungal lipase-type" evidence="2">
        <location>
            <begin position="97"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8150428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="296" mass="33680" checksum="99D2D85EE2654C35" modified="2022-10-12" version="1">MKFDILFVILILTVTTHAQDDDDFIIDSNSGYDETAATRYWYFCAASYCNPNVIVNWSCTTPCSKTPYMDDVQIFVNATNENAGFSGYDPKHNEIIIVFRGTLPWSIKNWFEDLNYIKTSFPYCTNNCQVHRGFYYSYLGIQDQVLNAAKRLTSKYPNAKLVITGHSLGGSLATHALVALTVNGYKVDHFYSFGSPRVGDSAFFNFVKSIYPAAKFRVTHDHDPVPHLPMEVQGFHHINTEAYYKDFLIFHKDVKICNDEIKEDSRCSNQNLVDLSVDDHCNYLGYNLAIGVLVCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1THM4</accession>
    <name>A0A8S1THM4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0200346</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1THM4"/>
    <dbReference type="OrthoDB" id="302814at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035743911" evidence="2">
        <location>
            <begin position="21"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="53"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8150572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="357" mass="40384" checksum="7F1A56869A8F7B70" modified="2022-10-12" version="1">MKSIILIALIAVAFTARVQERNMAKIQAELAKSTYGSALLHLVELHSMAGGPVQELIDAIEELINDLEEELETLEFDFQVRTNEHNALVVSLEQDIQDAVIDVNNTQDTLDNLLYPRRNQIQSKIDTVIGYQEENRKNYDEAVLVREQEHEAFEAQVAELNDATASVDDALALLASLTNPSLLQIKRFQNTLKNIENKLRSRSRMAPMIKALISLASNQNFSNQDVLKSIVDALNDFRNAVVDQINDLTAQEAQDVIDHEAYLEQLDDEFSEFGRQIDRATVDLTATNEKIDSMVEFRDQRAADQKQYTAELELENNTYAEETDIYTNTKNEFTRELGVSEQALALVQSVDFSNIQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TMR5</accession>
    <name>A0A8S1TMR5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0250239</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8154175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TMR5"/>
    <dbReference type="OrthoDB" id="10251809at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14733">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051568">
        <property type="entry name" value="LZTR1/Attractin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46376">
        <property type="entry name" value="LEUCINE-ZIPPER-LIKE TRANSCRIPTIONAL REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46376:SF1">
        <property type="entry name" value="LEUCINE-ZIPPER-LIKE TRANSCRIPTIONAL REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="377"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="300"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="300"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8154175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="530" mass="61561" checksum="D325C668CC6724A2" modified="2022-10-12" version="1">MDYCIRKTEMTDNKEDDVTFASYEWENIKATTASQLPTCRNCHTATTFKHYMIIFGGKEGEGRKKFCNDIHILDLKRLNWTSQIKISGQIPDVRMGHSAQNYYDKIVYYGGWNGYTVLDDIIMMTPSEQMNIVCIDWQHVKSENTPPKRQFHTANICGDFMYIFGGGDGKMWLSDLYKFDLVKCYWTQVETTGQKPQGRLQHSSVIYDYKIYVFGGEPDRSHQLNDLYQLDIENNIWTRLSPKGSTPSPRVSASAVMMNNKIYLFGGYDGQQWRNDVFMYNITENQWEYIVINNLDNQSNFRNQNKDNNTSQQSSPPRPRCRHSAIAYKNTIVIFGGNDSEKSYNDVYMLKQQSIIKLAESTLKQDYSSILFSPILSDITFFIDNQEIHAHKIILASRCEYFKTLFLNEQFNLGDKLAITDTSINVFKAILQYIYTDEVFIDSYIVYDLLALADKYMLQRLKNLCEDHLIKNISLKNVIDVVNLADKFSAQELKANAMIFLLDNKQKILNTQDINMLSKEILIELLKFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TMR9</accession>
    <name>A0A8S1TMR9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0270022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TMR9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8155311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="199" mass="23899" checksum="8DDB30BE948229FF" modified="2022-10-12" version="1">MIIIQNILNFIELTLVLFNYIYFYLMLNVSSSVKNTMEEFYQIQNGTILQELTKQKYQNCQQLFINLVLTIMIFITYAAHYQFDQQLSQNYLQLEQQVITLISQPLQNMEIFRKYFGVLITSNFSNHILSICEVDQTSKNQNSKEYIKYFMNLVQYRINHNCKLKLKKNCLSKKNQIVKYDQEIRLHGRVLMQQVLKIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TNW3</accession>
    <name>A0A8S1TNW3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0230280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TNW3"/>
    <dbReference type="OrthoDB" id="299178at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8152866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="319" mass="38154" checksum="C85D516E99578F56" modified="2022-10-12" version="1">MNQQSFVQVNLEHDQIKKRVNSLKFQLKDMDQKNYIIQNKLKDLRQLSPNVKKSPIAQLQEQLGLDRKLIIEQFKPIIEKEGIYVRNERLKTESYLQNSRNEEIQQKKIKVKQIEDQDSQVVRRKAEYHEKKINEVRQRQLQEMEMNKLIVHKKIVEINNFKKYEQQLLGEITLAKQKEQSLNSRFCKFVLNSDNSIILPTIQKTNSRMSQKSNSKQRPILGSHSVKRSEQFLDRFQRSDTKNNNNSFIDQEQYQRLKSELRKEDQNELNIGCQTSFIMTDKNQQSVQYSQNFDSYCSNNQDESFEKMLKNKQKQKCKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TQU2</accession>
    <name>A0A8S1TQU2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histone deacetylase interacting domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0260315</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8155105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TQU2"/>
    <dbReference type="OrthoDB" id="288083at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013194">
        <property type="entry name" value="HDAC_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039774">
        <property type="entry name" value="Sin3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031693">
        <property type="entry name" value="Sin3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346:SF0">
        <property type="entry name" value="SIN3A, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346">
        <property type="entry name" value="SIN3B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08295">
        <property type="entry name" value="Sin3_corepress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16879">
        <property type="entry name" value="Sin3a_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00761">
        <property type="entry name" value="HDAC_interact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0678">Repressor</keyword>
    <feature type="domain" description="Histone deacetylase interacting" evidence="2">
        <location>
            <begin position="266"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8155105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="872" mass="103040" checksum="D223DD8E0CD0ED48" modified="2022-10-12" version="1">MSEDILSRSNMGEFGEEFDQQLYERCEQFCQKLRRTNQQLYKQFSNIVNQNTKAAKEQLGQMLSEEPEILKEVNALLEDDVKFKTQKERLTEFINNESNKSKALETILKQIQNGHLDGHQNKDHILDLFSNEPQLQQQFLQSFKKKHDQIPPKKKIEENRNYFQKLLDKNGVVDMNELLFWDKFRMRVIQQYPNDFQQFMNEFMKIIHLYSECIITQMEVMELINQQEWLETEYIDDIRSMLSTRVIARRIQTPLFKPLKDTDFAQVDRVTRSYVRMPIGYAKANNNQEILNHSWVSVPFGSEDQSFLIMRKNTFEEQLFKSEDERFEFDVNIQQIKRTINLLQEIIDSNKEDPILIAKVINMRILQQLYRNQTQDQNEVLQIFQTKPAESAKILIKRVRSKLNELISARNNKAKQIWENVSLNNFHRSLDHRSFYFKKNDKLVINGQRFAREIEEYAKNVLIKHLGSKEHVFVKRKQDQIQYTFENSFLNSLAQMTNIRPTLPKQMPLITSIKDNVRFSPLISLWMGNEQILQECGQFVIHYLQIMGGNNSLDKRNGTIFMIKLINHFFQVPMKPMQISQLTLIDTKLKNEIQELEIYDIHYNPEDATISIPVIENESPGLFLNLQPRVKSTTYVTQNLYILLRFLHTIYQRLEMAYVISQQNKLGQDKRYNLFKSALFLSLKAKDFKYEDHLRSLFGKHGFIFSTLEKVFHDAAKQLAICASDVVTMSFFEKSQSVEGDLDKYFSVVSSIVNGEGIKVSDLEASVFCKFQELQEEEQLLKLHHAFARHKPEKKPIYRIACWHKACFISILPAYGGCNQDVCKSCKQPFLMRNMKRGERRMMINNSIEWIVDEFSHFKPINNWGEDYLKCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TRK8</accession>
    <name>A0A8S1TRK8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0250245</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8154187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TRK8"/>
    <dbReference type="OrthoDB" id="289265at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46809:SF2">
        <property type="entry name" value="GH21273P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46809">
        <property type="entry name" value="STROMAL CELL-DERIVED FACTOR 2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIR" evidence="1">
        <location>
            <begin position="182"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8154187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="235" mass="27821" checksum="000B4E3618D7720C" modified="2022-10-12" version="1">MGNCVKEYTNRSSLSDLPRKEIFQKDFSNHNVGHKRKKSRLLSQVQSIVIKEDPETEQDQNSSFELNSQFQIEQSATIHLIRLPEGLKMEKHHQIKLYNLHTQRYLHSHAIKHEHNKSNEVSTCNNCNYEYDWWQIIQINNKTYIYHPITKSYLKCLQTINNMGEVGCLQHDMDEWEIIADDEEIKQFSIIKLKHICTGLYLHTQPYQSKIENQHIVSICKPEVANALWRITDMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TUC5</accession>
    <name>A0A8S1TUC5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Insulin-like growth factor binding protein, N-terminal</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0270115</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="28293at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1081"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1125"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1149"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1178"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1210"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1243"/>
            <end position="1275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8155484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1297" mass="149713" checksum="F980E3BABEDACC11" modified="2022-10-12" version="1">MFLLITYLLKITYQQTLFYSAFTDTTLTNAENWSFIGINPLDVIQLCGPYSIVGGYEKFALGIAATKFLLLPPHYKIQISFTLFIIDTWDQNEYFHLYVDESLVYNKAYSYEQAFNNICGQTGNHRSDSFYEIQFEFPHTGTTTFIQLTSTLDQIPFDESWGFRNFRIFLYLCPSGCLTCSQSDTQTECKTWIITNTFFTETQYDSFSTDGWNILNGDYTKQQCSMIPSTCGYGVCGKDTILTLNLVSLPFHTRMKIKFKYLKIDSWEYNDYAILQVNQNLRWKQRLSELNQYLYQICGGSAQDTFINVELTFPHYLTSSTITISNTLDQIYSDESFGIRDIEIFVQQSICGDTIIQENEECDDGNLDPFDGCFNCVFSCVDGCSLCHKGICLGCYSGWLYLRYASTCEKISYINSASQIIAKSDTICYDYIEGCQECLNGICIQCQKGYQLKQESNDCQSICGDNIVTIDEECDGQNCIQCKFICPNFCDQCEFGVCQVCQQDYYLQNNQCIKQTINLCQTQCEVCIDSVCYKCQYGHNLVLGECQEICGNNSLALYSLDECTCNPFCLDCRFGYCYQCRQSYYLINNTCINICGDMIVSEDEECDDGNEIEFDGCFGCQFSCPLNCKDCQEGICLDSCQYGYQLINNECITICGDLIIAGTEQCEDNNKINYDGCYQCQYSCPLYCNDCINGQCLMCDLGYILYENMCTNICGDGVLSNLEQCDDGNQNDGDGCSSNCFVEINWICKQDDVCNYVKYPTLISEYIMQKNQYQYIKITFSQPVQTLSNINYIDTINASIMDLNETLFNITIMEVQPALFNKTSEVQYVMQIEIFQSLDYYPILEIKLTEQLYNDNQASLIFMIDYLQLKQPNYITEEQIQIASTFQQINEVSVKSLCLLTILLMLLGNALSFWGILDALQEQSYLKFINVLYPQTLIIYFSSSKVVSVQPALDTITLYKNEGKLLQFPYLQSYEKFEFYEINADIAEGFRAELFIFATLFGIYIGTSYGVKILQKLELSEFLSKQPKLIRLIQKIKQKVNAKMKDAKGNGLKSTLRACTWDLIFMALLELKAPHYFESPRSYICILIAISILITAIIILLNQVEGLKSWKKQNFHQFWQQKRDIFQVTKKFLILYILVFYQYEQELQTLILTLINAFYLVYIINFKYIDDNLEYVKTIVMETSLTIFTASTFVNWNIVSNYLLYSQRIIIGWIQMSILVSVLIIFIFIELFNTYSLVKKKIIESREKKNRQKINQKQKQNQEDLEQGIQKQKSTIIKEKTIFSRAHYTSKVQPQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TVK1</accession>
    <name>A0A8S1TVK1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0270146</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TVK1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8155527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="50" mass="5822" checksum="4BD1E515A787F499" modified="2022-10-12" version="1">MTAHKSRQDTINMINQGEFLEMYSIGGKKKYYIKQSIQQSDRITLSGRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TXY1</accession>
    <name>A0A8S1TXY1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TNFR-Cys domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0280259</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8156317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="298369at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1090"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1181"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1263"/>
            <end position="1282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1288"/>
            <end position="1312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1347"/>
            <end position="1367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1373"/>
            <end position="1393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1405"/>
            <end position="1423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1435"/>
            <end position="1456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1498"/>
            <end position="1526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1498"/>
            <end position="1525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8156317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1540" mass="178914" checksum="5FFEAD90A2A11C5C" modified="2022-10-12" version="1">MQYTDAALYYVQNFKAFILICDEYCDHCDLSTSICSICKLNFVLEKSRCVCKLILQGGNCQGTATGLYQYYTKDLLQPYTSKTQVFTDLNGFSYQFFADYNTTQRNRQIQIDGNNVGGFFIQNEYIQYSINQVISTPTDFTIEFILYLLGPIGLYSNIQIMVDSYIIGQITNYGDINNLLFSQGQIYNMQTCLVSGYSSCYKYNLILSLFQIDHLNTIQIKGHFNITDSKQAWAFSDLKISKMVWQSSSCTNFMYNNECKFIKCPSYAPQFGPNTCKDLLETYKFSEYILKLFYDYGSIFFEDNLSIFKKNIPSNTPQVYKDNFIFGGYQAWQTDQFQINLQCNPHYKARLFVKLLLIDFGINNLNNMTVYFDSSYKVYNWQNSVAVSLTEEKGDQLIKDYIVTISGTDFEVNHNDQELLVSFNCNAFNTAYCGLYDLFVIVALCPINCLRCIDSVTCLEIKQSLTVYYDCPQGYFQQSGECVRCISGCKVCNDKYYCNQCYDTHIYYNKQCFCQLNGLSFGDCQSNCHPLCQTCIAQVSSEQKYDQRTFNRKNVNLQCVSCDLSQGKVMNIQSCECQEGFYLDKNFQCLKCHDTCKNCLLQARFCIQCQDIQNRILEFQNCICKQGYFEVSDSYTCMKCADKCKTCEHFETYCTSCYVQQIRTLKENDCICSVGHYQYQNNLICSACNDLCEYCSDYNICTSCYDIQFRSLQIDKCICQNGYYQNNNQLICSKCYYTCSECNSSNEFNQCTKCPKSRIRSNIYLNVFECDCKNGYYDNNELECIDCTTYQYPPITHYCYSKCGDQIIQWNEQCDDGNLEPRDGCNQCYLSNNKCITDICLKCHLGECQQCIDGYYLQNDFTCQLCSPHCLTCISSPNQCIDCKFYTPQKQCIICMSTAGYTIIDNQCISICGDGIRTKEELCDDGNLDNGDGCTSFCTVEDGYICDQICIKIDYIDIILTESLFDKIYQSSRTIKLTFDQIVNITQAPLKDSISMITKIQNYKFITKNIIDKTQYTQEFNKIDIEITIELSESIESPDIIIIINNNTEVISQNHFSFQSKNQTITLLQYQALSDSEISNTKNLVRFNSYIIYLLIGFAILAFLLGGLDIFWNLLDTLQVLSYLKYLNVIYPYNLETYFQLFGFAEFDFLKSNFSVEDLISSSIEIEDPALKFKEEGYTSVFFVNVIAIFIVIMTTIATYILFHLIFYCIYYYAKKISTHQQISNYQSQDEPNVIVFLCFKLTRTMQKYFYNLKIQYKTGILRAFLTISYDLNLAIFLQLMSYSKSSILLILSSLFAFIFLFCEMIFIYFGIIVMSNKRFYFDLQEVQLKYGALYEGIELDSNPYSLYYNIVLFTKKLLFMFFLVFIYDYPLFQIGFISLLNAIFFTYIIYNKPLIDKSEYYKQIITEILLWITELLILLIGFGQQSEFFVYESYIKIGWVIVTILTLLIFIQLIIDIRQHMIFLIENYEILRILVNKIKQLHQSIFSKEIHINNDESQSLVGSSSRGRLKQSTSKLPSNQSSQKQVRKLVTFKMNDSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U001</accession>
    <name>A0A8S1U001_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0280217</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8156236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U001"/>
    <dbReference type="OrthoDB" id="10334827at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18773">
        <property type="entry name" value="PDC1_HK_sensor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="375"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8156236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="512" mass="61277" checksum="70D19A70C5AEB8DB" modified="2022-10-12" version="1">MFKRQWKKLRIRTQISIFLFVAQIFILSLIIGLLLVNLNFLEFYYEKASISILVQQELNLQTNLMNYSITDTKLLLYRSLNQIYRVKYLFQKIEMEQIKFLFPTFNCLQNFTNLDNYAFQYSFCYGEFGQAYTEPNQIEFYKNLTSSLTILLHLLDQDLDLSICTTTQEQYFAVWPGDKYIDYKPQTRPWYLSHIRQMQNNPNQDLFFSDPFVFWTWNVVMISQTLTIQSQNGGYQGIAATDLNLTLFQNLNSHFKRSNRFFTLRDGKILISNTNFTFGNYIYNESTIGLNYEDFIQIQHYLNNGNYTSNCLKLHKYLGYDIFCRYNQVFQQDQAIFARNLGISNLDLIIIFDILQNQEQILKSLDEIKERFLEFSAYSFFFIIIIIFLSFLINLIVIKYITRPLQNVVIQVQQYIRSSGFIFDRGNTRKIIKENNQDDMSRTLNKVFNNFQIKNNECQRQQLMISQYPINCYNFKNFYKISDLQLIQNSYLKQQEIIEMVLRLLQQQSQIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U1T5</accession>
    <name>A0A8S1U1T5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0310307</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U1T5"/>
    <dbReference type="OrthoDB" id="295039at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8158374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="236" mass="28096" checksum="D1334E70ABB70108" modified="2022-10-12" version="1">MLKKYLLQFNSKDYFQQKRVHTKDSEHTRHFEQHNYLKRSIATETKPALKPIDMEETRKEELTKFRSISLYNKKQIRMPTSAEKCCSNEKIRVFNIFNSTPRNVKPSRSQQQLNGFKEKVVYSSHNRQSSAAKPPISSRINDSQELFQKVSKPNMIPLRSFFKDNQVKQQQKNIKLELEKHANNNILELLLLTTGELKKKFEDEHKLRDSNSRQNSEERVRVKVRNSHKFPKDFFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U4S5</accession>
    <name>A0A8S1U4S5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0340025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8159850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U4S5"/>
    <dbReference type="OrthoDB" id="285923at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8159850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="150" mass="17578" checksum="27183644FE30D6FD" modified="2022-10-12" version="1">MNLCDNSGWDIEEQCYLDTLFTGYLPKLEKRISENSSHRIRTLSQPKQFNHSINSLHHHRPTQSQAFQMSSYSANFYTPSTTKTRIRFKFTPSLTQDEQSKQKNNSQSPIKKKPKYTIINMSQLLSVTPKVKIHQDSLNKVLERFSNKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U581</accession>
    <name>A0A8S1U581_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0310004</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8157796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U581"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8157796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="42" mass="4860" checksum="B8BDDB30917E820C" modified="2022-10-12" version="1">MSQSGSRIEASYNVYQNLQIISYYAVNVAKVVNRLKQEAIFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U5V2</accession>
    <name>A0A8S1U5V2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0320304</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8159077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U5V2"/>
    <dbReference type="OrthoDB" id="310806at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093:SF18">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035744657" description="EGF-like domain-containing protein" evidence="5">
        <location>
            <begin position="22"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="716"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="451"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="528"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="669"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="455"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="473"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="532"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="551"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="673"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="690"/>
            <end position="699"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8159077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="770" mass="86618" checksum="0FC6D58C465F6BBA" modified="2022-10-12" version="1">MFLSILLLSLINCQYEPVLIARFGMNKNKLVKDPAPKFECVDEIDEADEQPKEFSIKVGTLYQISDGTWYLKSTDPTSGLTNLLNLNDITRVKYVPKQEEDKKKKTEDKPEQVLKEFEWTPLIAYESFPRNRGGHSMHAIGDIIVLFGGCFLDIKCFDDIFLFDARTKEWTSPRIFGIPPVGRNGFGALVNGARLYIFGGYTLQGLTNDLFVLDLESKSWNQLFWPGIAPTPRAGHKMVLTAIGGIVFGGFMGEKYSNDVYILDIVNEKWLKPVVSGDIPIGRESFSMISHHGVAYVFGGYATGVVLNDVYTINEDLTWEKKEPAGKVPSPRQGAAFAEYDHRIFVAGGCNPKTFECYNDLYSLDTTTNHFSTVNAFKKKNLKAVEFAGMVFAGQLLIHFGGCKLDKTCSDSLTGVLLISNEQCPPCRNGGVCRVGHCNCQQGWQGIDCTLKVLCRQNCLGQGTCLSNGYCRCYPGFTGSICQLQIPCPSNCTDAEHGECQLDGKCKCKEGFSGQTCGEDPKEEKKKEEEICKNKCNRHGKCNYYTGLCECDYGFSGPDCSVVSSSDDYKSIWQLLKDDDNEPETGTTNVNMQDFISTGHKFQKQQNYYVSSEGEEDNYDENLDYNNLIKKQRKFNSLQSNNDQNISNYQLTMVPPKTEQEEDPYQLMLLNECPKRCSEHGVCIENECYCWLGYSGDYCQASKIADFYLGWRKKKVMHACLISFFVGMLLGYGVVLLMRKRGNNDEFRNMSEVPSQKIDDNEGEVDEIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U8W9</accession>
    <name>A0A8S1U8W9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Myb-like DNA-binding domain containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0350210</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U8W9"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019185">
        <property type="term" value="C:snRNA-activating protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001006">
        <property type="term" value="F:RNA polymerase III type 3 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042796">
        <property type="term" value="P:snRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051575">
        <property type="entry name" value="Myb-like_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46621">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46621:SF1">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="62"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="66"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="110"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="110"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="6">
        <location>
            <begin position="113"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="161"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="165"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8160864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="227" mass="27227" checksum="53A77F83D9ECBE0B" modified="2022-10-12" version="1">MYYNQSNPVYVFVPCYIPYQIPPVPKFDITKEEQQHSDSEKEQTKTIQEEVQTNASGIVRGKGQLWTEEEINNLVSYYKKYNGNWKFIIKHLKGRNISQCSQKYRKLIDQEKRTKKKWTIDEDKILLESFAEFGKQWIKISQKLPGRTSKQVRDRYVNQIDPAINRDNWTDEEDEKILQEFKKGGARWAIISKMLNNRSENQVKNRFYYTILKKYQGEQHPYLKVQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UBD4</accession>
    <name>A0A8S1UBD4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0370229</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8162158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UBD4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8162158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="78" mass="9058" checksum="7BB9BADF4134F498" modified="2022-10-12" version="1">MGCSINKKQSSSNQTNNTKITNDNFEQYLKSMGSLAESNEIRRAKASYQLQKNPIILRRMNKSKEKLDELDQDKMFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UC18</accession>
    <name>A0A8S1UC18_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0360067</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UC18"/>
    <dbReference type="OrthoDB" id="291346at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8161232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="356" mass="42948" checksum="4347BF4AB823A6A0" modified="2022-10-12" version="1">MDKDHFNVMNSKMNVQMNHNYFIDLITKANQTVRSLNQKLKRKQMRDKMDREYFNVLNGKINFQINDKEYIDLITTKANQTVRPLTLRLRMKQMKAKMDEEINISKLKHENIQQQQNNDDYYKNIFFLHKKRKNKFINKSMQNQDETKSPLTQRNFTHYSPQRQTQQFNKTMLPSSPMIQTTKIKVQQYQDYLKLNQQINQHKGQLNVQILNLLEQKVHIAKSLKQSCTKSGVDSYEQALKTIQVLKNNITQKVIKECNSIYEEKQNHKLNILEKTYAYFLRVLEVIQQMDDQIYQYMLNYKSKNPMNDQNSILEDINQMRDDAYNEKVRTFSKYHDIDQEKKRDRQKLNKVKAYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UGK5</accession>
    <name>A0A8S1UGK5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UDENN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0390244</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UGK5"/>
    <dbReference type="OrthoDB" id="206724at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032483">
        <property type="term" value="P:regulation of Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005112">
        <property type="entry name" value="dDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051696">
        <property type="entry name" value="DENN_Domain_GEFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296:SF21">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00801">
        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00799">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="1">
        <location>
            <begin position="5"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8163484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="634" mass="74835" checksum="2B131EF304C010A7" modified="2022-10-12" version="1">MTLFSEFLIIGYQPEEYKETEIIEYFHINGSSLPDYLRNVGIMCFTSVFDVKLEQEQQKKQDDINSKNYLHQFVITDQSGQLRYCTSLVVFESLKSDFYTPFAYVIVSEQSNLIRQKSYMRALYNGIFDRVTRYRDRGWQMGSQKLSNQQLFEFYLSVGITNLDGLYFVPKQIMKTRQEVITDLIINNPNHKNNLYNKDISFNALFSKLSIKSIIKVVQSIILEKQIILFTTQVWDLATITEAFLQFIYPLQWRCIYIPYLPAEILDTLHVSVPYIIGVHCNLKERVLTQYDCLDKILVDLDQDRVIGCNLILPFPEQLVETLINQSSKFSYSDDSELLQVQCCFLKFQLQMINNIVPYFIYSDTLQQKPKIEDIFDKDLYIEQFTPLDQEFYREFVNKTMMFQRFIEESYQYLHQKQFLKQNIKANIFIDLLLLINTSGYFNIYKLQNKDFEAKLFSLINESKQLIPESEKSIHSLFSQYQKQTNDYLIPQKKFPILKIDKSKIIQIDAIQNSIHQNSLMQRSTQSQKTDICNENKQVINILTIKVHKINPISKSYIQNDVTAQIILKYPKKSLSKLKKTLADQLLKLAPEVNVEQKPIQTSQMLSVRQSKRIFNTEEMGYVSMYRTKTYNMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UI80</accession>
    <name>A0A8S1UI80_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="8">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0410272</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="8">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UI80"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07834">
        <property type="entry name" value="STKc_MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000046">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000098">
        <property type="entry name" value="Mitogen-activated protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="40"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8164891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="419" mass="49188" checksum="60B659F80FD1976A" modified="2022-10-12" version="1">MIIESNVPQKIIDLYKPYKKQFESAKHVLQGHTFYLDQRYMPIDIIGQGAYGCVIQAIDKLTNKPVAIKKIERTFNHRLFAKRTLRELKILRLIRHDNVVDLKQVLLPPSREEFEDVYMVMDLLEADLSQVIKSDSILNDDHMRLFIYQVLRGLKYLHSAGILHRDLKPRNLLLNRSCDVKICDFGLGRAIAENIQNNIMMTYYVETRWYRAPELLIGYQNYNSAVDIWSVGCILAEMILRRPFLRGDSTKHQIKVIFELLGTPNEQYIQSFPDLKVQQNLRKIIQEYGHQNGIGLNQIFGHINQDLLDLLKGLLKFDHRDRLTAAQALKHSFLQILHSEEDEPISLPVNPLEFEFEKYELTKEQLKDMLYEEILLYHFSEFRNQYFQKLQSGQSLISHIVNNENARIIDPNADDDFEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UJ57</accession>
    <name>A0A8S1UJ57_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">60S ribosomal protein L21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0250112</name>
        <name evidence="6" type="ORF">PPENT_87.1.T0410067</name>
        <name evidence="7" type="ORF">PPENT_87.1.T0460210</name>
        <name evidence="8" type="ORF">PPENT_87.1.T0600204</name>
    </gene>
    <organism evidence="6 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8153950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8167063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000060">
        <property type="protein sequence ID" value="CAD8174056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UJ57"/>
    <dbReference type="OrthoDB" id="307981at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.70:FF:000001">
        <property type="entry name" value="60S ribosomal protein L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3260:FF:000001">
        <property type="entry name" value="60S ribosomal protein L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001147">
        <property type="entry name" value="Ribosomal_eL21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20981">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01157">
        <property type="entry name" value="Ribosomal_L21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="111"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8153950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8164505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8167063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8174056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="158" mass="18334" checksum="BE01C2B782672215" modified="2022-10-12" version="1">MTHSYGLKKGTRSKFAKPFRGHGNISIRKTLQTFKRGDFVDILVDGAQHKGVPFQYYHGRTARVFNVNPRGIGVSLQRRVRGRYVEKRFHVRADHLRPSKCRQEFVKRVQENDKKKTEAKKNKQVISTKRQPVQPRGATVVIPKQTTFQHPKAFVEII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UKE0</accession>
    <name>A0A8S1UKE0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0410131</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UKE0"/>
    <dbReference type="OrthoDB" id="305118at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051834">
        <property type="entry name" value="RING_finger_E3_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931:SF3">
        <property type="entry name" value="RING ZINC FINGER-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931">
        <property type="entry name" value="SI:CH211-59O9.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="188"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="255"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8164633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="302" mass="35880" checksum="F2321A906118ADDA" modified="2022-10-12" version="1">MDVEQIELNMAPQISDQNQGNEQVQSSQLALNAVMQDARQSYLLKKGRLQFLTLKRLTQAILIYRILYVLLCFIPFYKCPFECNNTLLNILLLICIGLELLKIVETIYQLRKVSFYLNLFTLYDAQQVIPFSLNDFQTIFALRYALDKLHKKAFYFRITINIIWVIYYIIYVAFFFTNAQYYTSEQNVIVSFMLIIISLDSVFFIFPYVFHFIGWFCFKCYDAIKKITSKNNKLLRSLKIEKYVEGQQLTDENICIICWDSFKKDEYYSRLKCNKNHIFHTSCIKIWIKTNITCPVCRSQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UQE5</accession>
    <name>A0A8S1UQE5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0460179</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8167001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UQE5"/>
    <dbReference type="OrthoDB" id="306606at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006913">
        <property type="term" value="P:nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027038">
        <property type="entry name" value="RanGap"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113:SF12">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="311"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8167001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="403" mass="46302" checksum="816AB79CCA887268" modified="2022-10-12" version="1">MDQKEKEIDEEQFKKEFEEKVEKRKQWLLEELKSCGKFLDVSAKKLDDLDALTIALYLHECQTELTVFHIGANQITDRGFIDMLTGLQWHDKLKEIYIGNNEITEIGIQGLIEISSSFKQLKILSMSSCSIDDSTFIQLCYALPELINLQQLQLPANNISDYGLVCFSTLLATNSLPNLTVVHFGNNPLTSKSLVPFFQALKKNKTIKILYLNDIGMELATIKQMACCLKKNKTLEDLNLGNTGFSDSAASILWPSLKYLKKLQLWNNHLTDKAIYEFINVLEREDIGLTYVGLQDNEIEEYDLVEDLNALLKQNEIIDKLTEQIEHKEEVEEVDEDKQVKQMIELAKLEEIEEKLGELEKLNEPQEEKQVVVNELQGILKTKKWAGEIEDPEDKTLKHSVME</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UQE6</accession>
    <name>A0A8S1UQE6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0460128</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lin-54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8166905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UQE6"/>
    <dbReference type="OrthoDB" id="6283463at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005172">
        <property type="entry name" value="CRC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028307">
        <property type="entry name" value="Lin-54_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446:SF34">
        <property type="entry name" value="PROTEIN LIN-54 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446">
        <property type="entry name" value="TESMIN/TSO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03638">
        <property type="entry name" value="TCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51634">
        <property type="entry name" value="CRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRC" evidence="5">
        <location>
            <begin position="152"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="92"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="96"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="131"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8166905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="281" mass="33263" checksum="896D90C2E27AD4E6" modified="2022-10-12" version="1">MARDYLQRQNSLIRGTSFISQNDEPYRPPQLQKFTSNLDEIGNEYDYRPIPFSRQTSNFFLYDPLTPLGPRRSITQQNNPLPLLSLQSQKSQIIDTKQKQEQRPKDLEQMSQEESDIEEDRVKQKIKKQQQQKDIKSREIQKKIKKRNSELEQQPCFCRNSGCLKRYCRCFHSGRMCLKDCQCVEGCLNNNDHLEERNNAIKHVNEKCHRNKNVPKDALFKLKDCFGCNCKKTRCQTGYCECFLRKSKCTMDCQCENCENGLDEAYLESQQKRKNYRAKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UZR5</accession>
    <name>A0A8S1UZR5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0500114</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8169019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UZR5"/>
    <dbReference type="OrthoDB" id="284523at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8169019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="60" mass="7399" checksum="9490ED070F742A5F" modified="2022-10-12" version="1">MNSEIIEVCQHQKDNILVKLKNGEKKWFKFDEVLKQQPKMLAQWLEKQVFFREVEEDKLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V3Z0</accession>
    <name>A0A8S1V3Z0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0560119</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000056">
        <property type="protein sequence ID" value="CAD8172158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V3Z0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8172158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="72" mass="8568" checksum="2E5BC25BC65CD1CB" modified="2022-10-12" version="1">MMIQVSNAQIQSIITMLNQYALMNLVNKRDYIAYNVQKIEFMFLILNIKKNCLKCLNIFNKLKLNVMIQLIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V625</accession>
    <name>A0A8S1V625_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0540251</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8171352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V625"/>
    <dbReference type="OrthoDB" id="311022at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="382"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="211"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="237"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8171352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="528" mass="61656" checksum="68A4B4F91B61ECBA" modified="2022-10-12" version="1">MSVRALYQSQQDVGRNIKSSKKKYTWKFSIDDKDHTIELFVSGLSGKKKVVHNGKTLREEASVLGSFNYSMTIQSNLLTISNNGDLYDLRINNMQFSHLYHNEKTKKEFQFEDGYNEGNNQEDYYSKLPKPSKQNKPLYKEQDQGYDFGLVDDIERMAPKKGLEEQNEFNTHSNYQQKLNIQFKPKDDPYAKFNEPKPQQRQTQSFSNKPQQPQSTFNQQQIKNDFADFGNFPKPQTTTTNENVFGNFGFGFNDTQQQQPKPQQQQQQNQFQQDFKPNPFGDFQQPTQQTQPTQPFQQQGQSFQQQGQPFFQQPPQQQVIEQPKPKPPLQTNLLDFGDDPIQPQQQSQIPPPQQQSQQQLLYQQQQVQQQQQLQQQPLYKNPINQPVQQNPQYAQAPQQQQQQQQQQQQQKVLPQDLLDAFDDPEPVVSQPNSQQQPQFNITSLYQQQPNVPIQSSIATQSPPQQQQFRQSMPNQYQPQPFNQMPQQQYQNPQISITQLYNQPQAQINMNVPVQINVKTQSTPFDDVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V9G9</accession>
    <name>A0A8S1V9G9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0570082</name>
        <name evidence="4" type="ORF">PPENT_87.1.T0570090</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V9G9"/>
    <dbReference type="OrthoDB" id="289935at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044157068" evidence="2">
        <location>
            <begin position="22"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="58"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8172416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8172432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="243" mass="28002" checksum="161604927D9E9038" modified="2022-10-12" version="1">MLKVAVCTLLVLSITAIDIKASVWTSHDQKMFAQIKQSGWGNFILNFGELHLSTGGILAELNTEIAKLIDELDEELAEVHHQYARRTDVHNREVARLEQEIQDKEREVFNAHDFYDNVLIPQRDRFAAQLEQLQENIAQNRKTLNEATVQRANDHADFEAKVAEHNEAISAIDESLRLLSQLSAPSLVQIQKVQKNLTKIQQTLKRHSTFQTFIKTLFESLWIKIKKLMKNIYLALCNIFLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VAI4</accession>
    <name>A0A8S1VAI4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0560002</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000056">
        <property type="protein sequence ID" value="CAD8171936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="307628at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8171936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="278" mass="32496" checksum="DA8FF9DBB4C77746" modified="2022-10-12" version="1">MKKKMADLQILXKSLTIIVTDLQNTLPNLISISYQIILGIDETFQQQPLALINSPYTSQEFAFIQSSWFLLPFYGFQYDGVQEIRFKKQYFSNVNNLWYDMNVGNFQSYATGCCCMPTLWHKQSWMKFEYITEFKPYEIGFISIKYFKDSKVDYLQSFKIEIQGDLQKATNLGLTRIIIDKAEQQTNLKIHTKCQEKETMKIILFIPNSTQNSITNPFFHSCLDSFEEVVITFTLISTSVAYQDLIVDISETECLISQLLFNYRFSQVDLFSIQKLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VCM7</accession>
    <name>A0A8S1VCM7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0570209</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VCM7"/>
    <dbReference type="OrthoDB" id="308861at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04369">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050935">
        <property type="entry name" value="Bromo_chromatin_reader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22880:SF225">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN BET-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22880">
        <property type="entry name" value="FALZ-RELATED BROMODOMAIN-CONTAINING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bromo" evidence="4">
        <location>
            <begin position="21"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="251"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="358"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="541"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="808"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="397"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="856"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="263"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="541"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="813"/>
            <end position="832"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8172666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1149" mass="135011" checksum="59197C896C9132E9" modified="2022-10-12" version="1">MSSKKEYGIEEHIYETLLDLYKLECSEAFRFPVDIHLFKCPDYYQIITDPMDLSTLTTKYKDGAYKTFNEIKEQIDKIIKNCRKFNTDPNHTILKTCSKFEQCFNTRWNKLNEIRIQLEIPDDYQLKVEMNKLDSIEQRKNKKKEPASLKEDQVGRKPRLPKNEIKTYNNNQFTFTKPQNEFVVNKFKIKSSSIQDDDQPYIDSNTSIKNESQNFQDNQFESKIQKKEVIESLPPTSFKVSISRMTSNDLGQNQEKIKETPPIQLNNFEPLQRQDSTKSTLSFKIPIPKLNQPQQDTIKDPLDFQIPNIAKSNQTQQQQQPIPKQIQLPIQQTNQSISTESQNQVQLQQQQQKIQIPTQQLTSQQPQQSINNQQSNTQLLQQPQVQQQPQLLGTQQKQQQQQQLQQSQQLLQQSQQLQQPQLSQQSNQVQIQKQQLQQQNIDDQKAIPKKVEIKKEQNEFAQQNSVASLDQQHPEILPKISINFNNPSKNTLLESKTQSYETKITIPKQQPQQLINTSSSQLPSQKQFEKSIPIIKGTTQKLEMQNTPNTPKGKRKIIDSDDSQDSSKIEQIPIIKKPSEKKIEYNETPIQHSISGPSSEKKIPNIGSHTFSLNEQDNKVQPKENQSFKLAPTTPPAIQPPQKIEAPPSKYVFKIDVNAIQKLENDHNQKKIEKNKKYALKLMSILKKHIIARQPNIEILNRCLEVPSKTTLNMILMWLKQEFDKIPFQEYITIAKFVELAYQSIQKFKCEEEPQNEQLLQQYFNKEKEQEQNRLKKIEQETKVQQEPIILEKIDIEMLSKSLRIRSKKKKVNDKEKKDRDTDKQKDSDKQQQKPVLHQQQEIQSFALENPKKIKLDQQQQQIQDIDDDFEEQTQAQDWWKILEKKKNECKAIEEEKKEKEDQEQEEKFFVRTLDQVKVNYTLNLGEKKGIGLYPTIVVEKMFFFLLNHQSTYFKQNKSLLTNFFRKLKENQTEKKNDFYQQFSKYNYYVDENHTQLMDLYKIDNYAEILIKLTLKDDGDFDPYQLENKLIINLGINNLLSYTTKHNFINYSSINKQFQLKLAQYPQSISETILLSKLYEMNEIFVESLETTKMNDNGGCGVFLWNQKKQALNQEYQYSCHLQIQVPFTCIQDLCLSLNDTVILNGQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VHA2</accession>
    <name>A0A8S1VHA2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0640070</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8175903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VHA2"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000592">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF206">
        <property type="entry name" value="HSF-TYPE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="3">
        <location>
            <begin position="5"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8175903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="295" mass="35280" checksum="E64467B9BC90D9D4" modified="2022-10-12" version="1">MIQKKCSKFISKLHNILENQMSDVIHWNEDGDAIQINDIEKLVKTVLSVHYKQTNFFSFSRQLNQYGFLKQRVGKNIYIFKHVHFKKTKKQLKTIYQREDLINIVSSHKIKSFKIYQKEFEDDNLVNINQQNSLTASLKELQNQQKIICQQFSQQIELYSQIKQIIIFLKKTIYECDHNIDNYCLRAQLHLVKAMNLIPNDEQHQQEIELIKQILEQFSNIYLEYDNQNSISQRSFNLSVKSHTPLPFYQNQQQNLKQPRLVDSKNTQLQQNISNYLIWLQQQYHEVSQLQGNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VIR9</accession>
    <name>A0A8S1VIR9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mei2-like C-terminal RNA recognition motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0660051</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000066">
        <property type="protein sequence ID" value="CAD8176887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VIR9"/>
    <dbReference type="OrthoDB" id="417481at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007201">
        <property type="entry name" value="Mei2-like_Rrm_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23189">
        <property type="entry name" value="RNA RECOGNITION MOTIF-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04059">
        <property type="entry name" value="RRM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Mei2-like C-terminal RNA recognition motif" evidence="3">
        <location>
            <begin position="133"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="28"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8176887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="257" mass="30696" checksum="0A0E3C035B6154D1" modified="2022-10-12" version="1">MQLAFSINPSCFGQFVRPKPNVQSKQSQKQDSDDEYKQHDLQSTTNIKNQLIMKNRSITICSNTSTLPETLSTSFHNQDINQSVLEQNKEDIFYKTMPTSFQQIQFKNKRKYEPQVQITQYQIKLDQIPGDQRTTLMIRNIPNKYTQPMLLENIDINHKDNYDFFYLPIDFTNKCNVGYAFINFLDSKFIPKFFLEFHSRKWKLFNSDKICEITYARIQGVEQLQGHFQYSTIMQEKDKRLKPIFKKYQSDQQFKRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VJ98</accession>
    <name>A0A8S1VJ98_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0670016</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000067">
        <property type="protein sequence ID" value="CAD8177087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VJ98"/>
    <dbReference type="OrthoDB" id="284091at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8177087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="76" mass="8783" checksum="550A4B135975B929" modified="2022-10-12" version="1">MSKKMRLNQICSLTGDQLNHLLSSPSEEKTRKTLKKVTFDNYALYLQFRATDCPNQVNSTLQQHHFKRSNSANTQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VK22</accession>
    <name>A0A8S1VK22_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0620141</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8175046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VK22"/>
    <dbReference type="OrthoDB" id="301999at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8175046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="481" mass="56850" checksum="B7C1B4AE2D50D492" modified="2022-10-12" version="1">MKNYYSFAKLFAQIGQSRLLQNPINSRIQAKKVSQYYNNPLPINEIENLLKSLHYFIPNEVIQIMKKSHKFKQVFGKQEFTQYFKSQSLDSIQKADLILLCYKLNLEDDLWMKCLTMKIDDLTPYYLSETMWALKNKSFQLSENQEKSRKLQEEIISFIPSKLNQFDFYSLSLMLQSLTKFHDVNWDDLAKTFLKLINNNDHFSLCYGLYALAKQRSRLTIKINDENLIQIVQNEMRNFSQNDLLLVLWALQKLNLGSEDFWNYAFTILQTQKLQTLQSIAIQSQILSQLFKVDEVSWNKIEKQAISLLKSDEKSLVTLFQTFSINNKGSQQFWEKICFQILQDITKFKLESLIKLASHSDIPELSKKIPLDWTKLTISQRCLIFNKFAKLGLQIEDKYQYILEKIDQVFGKDLVLVLYTIYRYSPQLNKDPIINQLLISMDHLNLIDIEQLLELKEIPQEVNIKLQKLFEIKMNKKQKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W0I2</accession>
    <name>A0A8S1W0I2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0780162</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W0I2"/>
    <dbReference type="OrthoDB" id="283793at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8182373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="305" mass="34302" checksum="30EADA5D942DDCD6" modified="2022-10-12" version="1">MATPQSLSPAPMLSLGDFSAKKLVEDDFSTGKVTLKVKASANGGGIANYKGTLDISKSLTPGQETKFQFPYNNYYFWFATRKEQTKVHVDFGKFNIIKQQNLFANVQFYNSSSKSTIRFGSVYEGKKCLSHARFEWLNQDKVNFLLRSQVKHNNWVYVIASQLCLSRQEFDKLDFLVGYQTQNYDVYLRHLTNQIPSFPLFSNFGFGKFLVDAVYRQNSNQYGVEIEYTPVSKLSFQLAASTKFQGADVKARLNILNKTVGLSAKGKFNNTFSWTLATQVPVDGSSPSKCGILPIPLGITLETTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W1D2</accession>
    <name>A0A8S1W1D2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0820005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000082">
        <property type="protein sequence ID" value="CAD8183834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W1D2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8183834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="249" mass="29524" checksum="F699ADA85ADFBA63" modified="2022-10-12" version="1">MSQNFCIKNLQSKIFQQLFQTISQNKSKQSVLNYGYYVGRLWSRQNNDFKQVRRSTQVKFFWISILIQKQPLEYNIIHSQEIMLNFQQGILQDRKSTDPQCQAMKKKQKLRLWFMIVQMNHLFFLHIDKWIEEFVFQAGANVKIALIGHTQKLTEQYDIAYQIQINHQDLEFKDKMNKLLDNISISTESILFLYLGLIPLNQQDQSPISNLNLQDNNDQVNVIEFNHQKDQLIEVLASNNNQEVKSCEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W2L6</accession>
    <name>A0A8S1W2L6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0780246</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W2L6"/>
    <dbReference type="OrthoDB" id="307728at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8182512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="197" mass="23092" checksum="DCFFBAEFFBC5EE95" modified="2022-10-12" version="1">MKLIKFDKFDKPCLQSQHEIKITTNLIRTPKQSQSQTFITFKSSPSNSNKNSIHRQSTHLIQHKSQFSQSYKSQNQAFSQQNTISTVQECISILSDFKSTSPLLLSNKKVTQKTKDKMNQISIIIDQMLPQDNKEEMRRQKNMNQEKIQQMMKLLMQEKQQKYQAIKSGDSLIKQQQEQIQSLKTRISLYRPSHDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W3I1</accession>
    <name>A0A8S1W3I1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0740232</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000074">
        <property type="protein sequence ID" value="CAD8180656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W3I1"/>
    <dbReference type="OrthoDB" id="290214at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045913">
        <property type="entry name" value="TBC20/Gyp8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20913:SF7">
        <property type="entry name" value="RE60063P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20913">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 20/GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="443"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8180656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="882" mass="104459" checksum="531E0540A36F9113" modified="2022-10-12" version="1">MLKTPSLKSFSNPSERNQSEQYIQCYTNKIVRQVQQLNSSYYEEDQFDQCQNTNHIQQFEDYMQNLVEIISNPPNSQYLSKYYVLSTENETITFISETHPETLMDYIKLRHQNNAPMNEDEIVQLAYAILNGLSNINILYLSPSYIIKKWKIINYMEQMVHQNQRQFLESEDKLYLAPELYKYLDIELNDCDDFNKHLSQEINAPKASVYSLGIILLQCIYFEFKKDNTNNYQDILKNSKYSWSLRDLIQQMLREEDERSSIKELLSNPLFYEHKQIEIQPKQSNQMSLAVFFQFVKNKFFNLDWADQFFSMEEIIYISDLKEGIYPIGELKSEINSQITQIIQNCPNNCTLEELIATLTKIANPQQQEENQVIQPKKQQFLFRKWFKKQEQKSEDMQEQVLAPQEPTYMAQVLLLKLISQASEKAFQTFISKVIHFMGGSGLIWQNIRKAFWPRALGFESLEAQRQIVQLTLELCEYLNASKEKDVIQLEKDLNRQKYSKRTQDAIRITIRGFINQSVSNCYIQGMDSISYILLEAFNYDSELSTICLNEIYKKRIVNLPPEEEQVEISEYFGQKLQEKILLYNWTLQYFDPGLSNHLKETDFKCETHIVSWFSTFYAREFNLENVMKIYDYFLISDESFEILLACQIMLELKSTFEIKDSEGMLSCLKNLQNNIQLDNCLQNSLRFSTQLHKTFFILTHQDEEICNQMKEENEYLQERPWEHPLTFKQLQEQLTFSISIHDFQQLLREQKSSFNVLSLDMRSAKDYEQACISGSLFAYYDKKKINNIQLLNYFELRGLVEEQNIYYIVVICDSEKQNVKEIMDYLLKQRIKRLVLLKGGIQAALLDAQDIIKVKQKAVLPPWIKLQIDELKKKNIQSKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W6D4</accession>
    <name>A0A8S1W6D4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0820105</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000082">
        <property type="protein sequence ID" value="CAD8184032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W6D4"/>
    <dbReference type="OrthoDB" id="291946at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03693">
        <property type="entry name" value="EF1_alpha_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03705">
        <property type="entry name" value="EF1_alpha_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000003">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000005">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="8">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8184032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="336" mass="37042" checksum="F47640EF30FC1204" modified="2022-10-12" version="1">MITGTTQADIALLMIASPAGEFEAGISQNGQTKEHILLAYTLGVRQLICAINKMDERSVNYSKGRYEEIVKEMTIYLKKVGYNTDNVPFIPISGWNGDNMLEKSINLQWYNGPTLIEALDAVTPPKRQTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLAKGMIIQFAPSGITSEVKSIEMHHQDHPQAIPGDNVGFNVKGISVKDLKRGYVASDIKNDPAKECNSFNAQVIIINHPGQIQNGYCPVLDCHTAHVACKFDQIISKIDKRTGKVIEEEPKFIKSGDSAIVKLIPTKPICVEIFSEYPPLGRFAVRDMKQTVAVGVIKSVEKKEIKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W7B0</accession>
    <name>A0A8S1W7B0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0770046</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000077">
        <property type="protein sequence ID" value="CAD8181736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:002297">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF98">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="1"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8181736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="773" mass="92481" checksum="72A740C809CCCBD7" modified="2022-10-12" version="1">MQDNQTYIQKTYLQNKNEGLNAIKMLAQIKNQFILQVIEYLENEEQQLVIIQEYCDQGDLKTFTLQHIGYKMQESIIWSLFLQMAYALLQLNELGIQHRVLIPENILLLVRTEGYQIRITDFHKSSRQTLCQLPCAAIYLPYEYFFEGHYTQKSHIWQIGHILYFMVSQKLLFPTTTLENQVKEQLRRGRIQINIPDIYSPGLIKLINVCLTIRENQRPNILQILRMKEVKDALTRPYYEFSLDQRRHLMYWINSSETAISNPVQFTFNEFDLHSPRQIQRDVVVKKNFKNTKLPEIFQQNQNAKKSSTDRLPESDNIRTETKRKEDLYLQQQLLSKTYTNKYFQGVVKPISFHKKKQIINNTELPKFSIPYQKQLDPVFQNLLENKKQLCDRWIGESEYMFLRSQIKFNKTDKLLNYLQTRHPKVDSSQLHQLIYYFIINXDFHKSSRQTLCQLPCAAIYLPYEYFFEGHYTQKSHIWQIGHILYFMVSQKLLFPTTTLENQVKEQLRRGRIQINIPDIYSPGLIKLINVCLTIRENQRPNILQILRMKEVKDALTRPYYEFSLDQRRHLMYWINSSETAISNPVQFTFNEFDLHSPRQIQRDVVVKKNFKNTKLPEIFQQNQNAKKSSTDRLPESDNIRTETKRKEDLYLQQQLLSKTYTNKYFQGVVKPISFHKKKQIINNTELPKFSIPYQKQLDPVFQNLLENKKQLCDRWIGESEYMFLRSQIKFNKTDKLLNYLQTRHPKVDSSVILQQINDIADLEEQRSFYKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WAS2</accession>
    <name>A0A8S1WAS2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0850123</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WAS2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="102"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8185515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="435" mass="51877" checksum="157A72B3DCEDEB84" modified="2022-10-12" version="1">MSDYEFGHLHQSTLDKIIPGNILENLDEYLNQFPQRYNNFIQFMRQKKLQQIDDQFSIKSRLSERSGISSISIQKFKDQYEQMLMNQQQSDQSNGFNHYRSQAQSQTGHQQQTQMKQSHKILRSESKKPSSRTYFGKQSHPQRVSHDVQIFKERVSVKPSISKFDTFYDSIKLQKILKIAALSKNISYKFIMCYFWEQMIEDCNIIMTNYNKYILQIDTYRGILRQLGVSKQVFIEDDYQAEIFFEQQQVEYGSMFEKYSFISLKESQSTNIYFSYKKLDGSIQKTINLKCQFRQCIYNYSSLNQISYNTALRILKQSSEYLTDQIKNKKKVIEFPKVDIKSIQALRTNKKSIKLETYINNHSKYKFIYSSRLMMNILFLFGRLLKTNRIFNQNVNQICLRQHSSYINASTMLIRIYNWIKIVINLQKLFNNKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WBN9</accession>
    <name>A0A8S1WBN9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">cathepsin L</fullName>
            <ecNumber evidence="10">3.4.22.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPENT_87.1.T0870187</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="11">May be involved in extracellular digestion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Specificity close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity.</text>
            <dbReference type="EC" id="3.4.22.15"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.15" evidence="10"/>
    <dbReference type="EMBL" id="CAJJDO010000087">
        <property type="protein sequence ID" value="CAD8186661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WBN9"/>
    <dbReference type="OrthoDB" id="190265at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000104">
        <property type="entry name" value="Cathepsin L 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035765977" description="cathepsin L" evidence="12">
        <location>
            <begin position="25"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="14">
        <location>
            <begin position="36"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="13">
        <location>
            <begin position="118"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8186661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="324" mass="36253" checksum="0E99D8764A890415" modified="2022-10-12" version="1">MEKSLISVGLLLLIGSGLYLKQNGEDLYELQKVKQFNDWKFQYNKKFSSEQEEMYRYLVFQQNAQLIEAHNNDKSGKYTYTLETNQFADLTEQEFVQRYLSLKPKNSNKSKSFDYVPNGQARDWVEEGKVPPIKDQGTQCGSSWAFAAVGVLEINGNINFGLQSSLSEQDMLDCSGPYGNQGCSGGWMDSGFEYVRDHGIASASDYPYVGKDQTCKTSIQRDFKYVTGFIDVDGCEELQTAILDQAISIGVDASNWSFYKGGIFNNCKQNLTSGSILVGIDQNGVWKVRHQWGSKWGENGYIRLAPGNTCGVCAAASYPVLQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WCW9</accession>
    <name>A0A8S1WCW9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0870058</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000087">
        <property type="protein sequence ID" value="CAD8186405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WCW9"/>
    <dbReference type="OrthoDB" id="440673at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09804">
        <property type="entry name" value="Dcp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010334">
        <property type="entry name" value="Dcp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16290:SF0">
        <property type="entry name" value="DECAPPING PROTEIN 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16290">
        <property type="entry name" value="TRANSCRIPTION FACTOR SMIF DECAPPING ENZYME DCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06058">
        <property type="entry name" value="DCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8186405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="124" mass="15099" checksum="4E180F2D1EAE024F" modified="2022-10-12" version="1">MQRDQLNFSYLKRLFPTLEQILYTGKFTSLNEFDKCTQLWHQAGFQGPFFLIKLTDQCVFIILNQNSTQDFIRTIKKGLTFELNKGTQWFYFHFQDEQQKVFNIWFQEQQDFENFKVCLEKIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WEZ6</accession>
    <name>A0A8S1WEZ6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Histidine acid phosphatase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0870232</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.2"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000087">
        <property type="protein sequence ID" value="CAD8186749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5821688at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050645">
        <property type="entry name" value="Histidine_acid_phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567">
        <property type="entry name" value="ACID PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567:SF211">
        <property type="entry name" value="PROSTATIC ACID PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="725"/>
            <end position="747"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8186749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="757" mass="87482" checksum="43343F9CAC732C51" modified="2022-10-12" version="1">MYQLVFSFISFVLSQDDLISVQVVWRHGARNYFIPRGDEAVLTPTGMRKQYVLGKWLRERYIIENQLFLSSKFNENEIFKESSDENRTLTSAYCNLQGMYPDGPSIPHFQKENESLLLPPNQGAQISKEIGDDALPHKNQLIPSHTKQKPLLQEIVIQCPKMNAKNKETQLYKEVNDATAQLYDDFNTQLNLQGEETVNDIIEFSHWKDTFIFNRQNLNNQILLYNGDPLPPNLKAKTLQQLDDLANLAQSLERFQTLDQVKIQSTPYFQRLIENFDSALNGSQYFKYFGSSAHDTTLGCLLXGQPQLLLINVSKQILIRIIYMYQLVFSFISFVLSQDDLISVQVVWRHGARNYFIPRGDEAVLTPTGMRKQYVLGKWLRERYIIENQLFLSSKFNENEIFKESSDENRTLTSAYCNLQGMYPDGPSIPHFQKENESLLLPPNQGAQISKEIGDDALPHKNQLIPSHTKQKPLLQEIVIQCPKMNAKNKETQLYKEVNDATAQLYDDFNTQLNLQGEETVNDIIEFSHWKDTFIFNRQNLNNQILLYNGDPLPPNLKAKTLQQLDDLANLAQSLERFQTLDQVKIQSTPYFQRLIENFDSALNGSQYFKYFGSSAHDTTLGCLLSALNLTSAQCQADIYLQKNIKQNKQFVTILISELYNSINNGPFVKVLYNGEYVPIWSDFAKTCEYARFRSIITAYLVDYKKQCLQSDEETYPTSYQTPKWAYIVFIKVILFLIMVLSAILYLRQKIGQKDQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WH12</accession>
    <name>A0A8S1WH12_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0900109</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8187917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WH12"/>
    <dbReference type="OrthoDB" id="298460at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035931647" evidence="2">
        <location>
            <begin position="16"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8187917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="360" mass="41238" checksum="259E3725F4F20C3B" modified="2022-10-12" version="1">MKLLVLLCIVTLTFADQRIDFFNKLSQSEFGKTILQTIQVQENNVERVLQFIRQLQVDINAAQTEHTNYLQNRNGQITQYINEADQALAKAKQQKAQDEAQLPLKEEELNDKRSQGESKFAEKQRNLDRIASLTAEREESKKAYDQRRSEIVGLLAALNQGKKLIQQIKTGSVFTQQEIFADIEHHHKKFIQRFPDRRGFNSLVSLSLAMAQDSTLKSDQSALERVVQVIEDLADSLFQLQKQEMEADDAREAAFQQAIARLEIANQSLEGAVAYLHAQILRLEQSLLELQNDIATQAQMIVNKQNEKADWLNIQRDETKSYGKQSENRKSQLDLLGETENVFSKGPLTPEQQSFLQHLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WI27</accession>
    <name>A0A8S1WI27_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0910053</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000091">
        <property type="protein sequence ID" value="CAD8188267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WI27"/>
    <dbReference type="OrthoDB" id="308616at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8188267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="294" mass="35017" checksum="792ADD2D164E737C" modified="2022-10-12" version="1">MKINFELTPNNCVTFYERQQKQLIDYCISDDQTNIFAIYHPRNIIGWRTKDLYKFYNITSTLNELSLICIKNNYICVASFKSSTVIVMNIKQEKEIINLRHRNSNMKYITFSKNSKYLFTINSEKILTIWNFNREEQLSQINLEQNFKYFVINQLSLITTIKLYNKNQRIRKRLNQDQIKDQKIKHLQISLCDHLCLEIVKNFQINVISLHTQKLIRKKQNLMGLSCINFINNNKFFYIIFENGNILLFQTSTMRILGIITNIYNYTNIIVSCFNNKIAILANNLFGLKLWIVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WI93</accession>
    <name>A0A8S1WI93_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0910139</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000091">
        <property type="protein sequence ID" value="CAD8188435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WI93"/>
    <dbReference type="OrthoDB" id="290656at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035813542" evidence="2">
        <location>
            <begin position="19"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="88"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8188435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="351" mass="39710" checksum="C4E24B6BE8C5C118" modified="2022-10-12" version="1">MKVIAVTLVLLLAGNAFAGDLIHKYPIIGKSKPSLVSILTELESKLTTGGSVDTAVNFLDSLRASIDSEQIRHDQLYTDQRNQCSAELELRTKDVKDAEQVLNRATEQYENCSTSKKKADAELDNNLDSQKATDTDIKILDSIRQAGAQNYNAKKQDHIDALRSIQESLKILDSFQSGGASLAQMSEISLRMIQDAVKLKTTNIMSQISTIFAQMLTQNGGYIEVFERLKQLLQNLEQNLYTNLQKISEEEDQSIQEYDDRRVHLVEYYNNLKRTENKLRDHITTMDICQDQQSAIIDSARGKKVRNGEIFDSATRMCNDFASEYEKATSVRKQELDLVDRVRSKIIERGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WKK2</accession>
    <name>A0A8S1WKK2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0950118</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000095">
        <property type="protein sequence ID" value="CAD8190073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WKK2"/>
    <dbReference type="OrthoDB" id="299023at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8190073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="335" mass="39641" checksum="1E0456B220013C86" modified="2022-10-12" version="1">MIKQLKKDIQGLPNMDYEDNSINLYNYILNRKQEYPQLSNTITRPLNKRTRTFSFDLQKPSPFDIQKKQLRFGSRQGTRLRIKKEQQIRRRSCYCTNCGYDNTKIIHLMYKGAICHHQLEYANQQNKKKQFIQEAILFSNKLTKKLNLLKTRFSINNTYIKKRCNSCDTHIPYEQLIKAKQIHQQLLQRKDTHTKTQLRHTFLVKRQQTINDKLLLIPIKTLKTVQDTQIPIKSLPTQPTNSPLKAPKKKQLNKTFVPVTRSILPQLTPTSQFTLSSNKSIDFQLKYQLNQSIKKLQKSAFVSKKNSLSNITNPYRNISQLDYLLKVRRRQINNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WMH9</accession>
    <name>A0A8S1WMH9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0940010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WMH9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8189409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="286" mass="33395" checksum="40B65BD4F2805C31" modified="2022-10-12" version="1">MIVPKLQNVTEIMGLAYNFNFQIQFNTKYVLPKQGVIGTTVSVTYLMVNALLYQERIKLNVCNKMYIALVVIELIALLKILITVKQILKIHPVLHLLEGMEHVIGIIQPINAWLSILAINYHNLHPKQDLYLVIGMLHYVQLQLVFNKCTFVMKLSSQNYIQVCQLQNNVCTQITDVLSLSYPHKIKQQYGFHNLQIMEVFVILVLLIRFLLTIFQQVNIINMLIQKILLNGLLIYYKFKKSNLLFYISYRAIVIMPQVNIVFEYITYVPNNLVHKFLAIKFVHKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WNK2</accession>
    <name>A0A8S1WNK2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0940216</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WNK2"/>
    <dbReference type="OrthoDB" id="282886at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8189799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="269" mass="31376" checksum="110401E5F35A6C2C" modified="2022-10-12" version="1">MSRPRLPTVHNEASLNKEPIETVYAIANRYHTDWGLKEMPDMKIYPDVCKVKRLTTKPVKPPKRGTFIEDLQEYHSKLPGPKYEYQSFWSEPTEQKKTKESSKYIEQNQRKTIISDIITDEKRYPRPGPADYNKEFKSKSIENEPIISQRLKQQHRTNYFEDLISQQMNVPGPGAYNTPSRVVSEHVIKKTKSRSPVVHYPGPNSYSPDDPTYFTFDKKSKEQSSRKPVKNLKKYNSSRPGPVLGQSDWNKGWMSKVSKAPQFSIYYDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WPR1</accession>
    <name>A0A8S1WPR1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0980133</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000098">
        <property type="protein sequence ID" value="CAD8191202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WPR1"/>
    <dbReference type="OrthoDB" id="415460at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="343"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="387"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="444"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="489"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="521"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="8">
        <location>
            <begin position="627"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="88"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="157"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="88"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8191202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1252" mass="146303" checksum="832FB0C546D8E593" modified="2022-10-12" version="1">MISRDTGFQDLESGIQSNNLSSGQLVDLDQNSPINGQQLDPTLEFSQLQQLEALRMKRDIFDNKHKQALNLGNKSDINEEDCLISNFSGKRSEPQGSISSISCNLKKQKPVTSKPPKQRSNKTIQFQQKLEQDDFVIGSPKNQSGTNILGIKSDAKIVKYQTFVQQLEEEQEQNEKQKRAQMHHDTLKQQSLVLRGKSREMKTGLNEVWSQKALIIIRLVSRFIQQLKTKTERIKFRLITQRIFEVICDNSANFEYMLINRLIKQKPSLGLVIFHHFSSRALTILNTIEYINDFLSKYIKVIKPDSLFKIVWDIILLLFIVVNIFYIPIYISFDVRSSGLFEWIFDLLPSWVFIAEILLNFNTAYYDKGLMHEDRKQIMKHYVKGNFFWDIIVVIPFLISYLDIPFVRYTLLLRLTRLSPLMTSIEEVLNLEDSLQIVLDLLKLIFFLLLTGHFCGCAWHWVAIIEYQDYGQELTWLTRYEPDAMNYEWFDRYIISLYWSVITTVTVGYGDIVPVTTVERVFVIVVTLLICGVFGYCLSNIGNIFKQITDKKAVYKQRIREINQHIRKRGLSYNLQLKVKKYFEYFLKVKQEEDQHAEQFIEQLTKHLREEVLTDIYSKTLKQSRFLRENFSEEILNRLCQIVKETKLYPEQVLFQRNDSPKALWFVLSGAVEYVADHQNEDEHYYTETFLKKLTQGAVIGEREFISQTPYEYNARATKFTQLLVVDYQQFYLILQENNDEFEKYCLAKDNLLFNSNYKAFGQICEICGWTHRFIQCPFVFLQPNKNKIASSFVSTKTNKRLSFPYRTLAKTNWRNNMPEVQEAALGYIVLNNIIPEKEINDNYLVNLGFELNERDEEPSKIIPNKKNSMSKDPKLLSQYDNTLQQQPIQQPSIQTVLQDQKSQNQTAIFHKDGQKVNLTNESFIDWDQGSQLRRSMNRIKFQGLDRGRTLQSIKGKQHHQNNGGGVDILEESMIEEELKIQNTNIGSQKFIHSKLKMVKSKHNNQNPIGIRKVSWLEFDSGPLQHQDGKAAQQLFNKQISKGSETNFDQSPPINQQENDIKQRIEARENSIISKKTNNNIDITRRKKRRKTTQLLQFFQGMDNGDTKSTKRVEKSILGNDVYSSSVYTTGGPSNTALMDSNNKDNNNAENNSVHVAHSFDGIQKRIQDIVHVHFEMDLDRYKSSKFYFPDYNVEVVLQKISVYYDKVNDKKFDMREMKRTKTNLTLFDRIRQAKNPTIRGSFVDKSLQDND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WTL0</accession>
    <name>A0A8S1WTL0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1030125</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000103">
        <property type="protein sequence ID" value="CAD8193138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="295267at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8193138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="282" mass="33355" checksum="B74B00EA6B6904C6" modified="2022-10-12" version="1">MSSTIVNKMEEAMNYLYQILASQSEVQLPKNYNQQTNICSTIIVFQRYVSNTQTIFLYGYDSNSLIIIKQQHYKLDEFIVNVPQYFIRVQQFLSSILIQYQQVCIHFINYASLNECDSIQIKQEAYNLLEVPYPQSKIIVWIEYQKNLQFPNDCLQIKFPDNQLALLKIQSNLIKNYEMYEEFTIKQITQIQGIHKQFKQKIELCEKQVVTFNFLNLIKSITLLQDMPDQIQYQAQLLMNLPSYTGQDVMIMIAKLKLYKQNAILSKVINQQNLXTKYNTKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WZ12</accession>
    <name>A0A8S1WZ12_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1040114</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WZ12"/>
    <dbReference type="OrthoDB" id="304484at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF13">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="16"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8193495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="608" mass="71263" checksum="B3730EA98C84EFE4" modified="2022-10-12" version="1">MGANSSKQNLEILNEYNFLNVAQDDGYGEIQLFSNKIKRTDQMALIKFYDQSDMEKRANLIQMRKQQNNQYFTKILDYQVQTTEDLCSNINLLHVGLEYLEKSLQDDILNRRANDRTYNEGELWYLTQTILSGLKDLQMNNLQYQDLHPNNIRIKLNGEIKLLELCCLANQQSAYQKVFTQLREVEYLSPEQLYVLENERQNDKQEYSIEKQNVFILGVIFLVCITNMDVKKYYNNFTFNSELANQKLNSAFDKYNYSDPMKHLLKDLLQLNTYHRPTYLEILDKISHVDSSRISPNLYALNMQDQAIKSTLHFKNQSNFTQEQIIESQPQSLYEQHNLINDSFNRQVTSNFQANIESAHITEVNKQNYDFEIATHSKYESNHQLTPLTTQEGLDKIRQLKERFQLMKGEVHQQQAATVPTTFTHQDESDTYRIPNQNIQNPYQPNFLDMDPIIQRALQKSREVMSKCNPYQTVDPYVPILSSSNASFLPQPGIPSIIQPIQNSQLDNSQFNQYYQTSQQNPQFQSQFSQYNQINQVQPPSISLQQSQFQKQPTFSQPFSPNPQYIEQTSPLKKPSVSQYVQQQTFTPPSFNQQQSMYQPYVRNDGFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WZU8</accession>
    <name>A0A8S1WZU8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1080007</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000108">
        <property type="protein sequence ID" value="CAD8194882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WZU8"/>
    <dbReference type="OrthoDB" id="2335338at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="249"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="283"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="385"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="419"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="453"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="487"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="521"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="555"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="589"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="623"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8194882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="706" mass="83188" checksum="CF9B9063AAD7C5EC" modified="2022-10-12" version="1">MNQKFQQKLDFKCKDHPQTSAIFWCKSIDCRENRIFCINCQKQQKHYQHFIDDVDDINQLNQFLITKSKYSKDLITECQLQYKSTVQSYEQLISGLNYKFRIQEDKINEFEPNQALTALDSLVLFDDMKIHIKSNIIGPLQKIQKILDDLYMQLELQTIKYQISDQQRATNQIDYQKGSFFLYLFFLAISLNIINKSNDAIQIFDKLLLIDPFNLEFMYQKANSLNKLNKQQEAQQIYTNIIKINPQHELSYQEIGDILREQKSYQKAKEYYEKCIQINNNNSASQFGIGECLKETYKYNEALEYYIKAIQISQSNLLYLQSQGDCLRALERYQEAIEAYNVILSNQPKNADASAGKGICLLYLNKYKEAEVLLKNSFTLKLDCILGYYGYGLYLYFQDQYQDAIKYFVKALDINPQHIHSLNWKGNCLRLINDYQQALEWYQKALDINPQYVQSLNGKGECLRLMNDYQQALEWYQKALDINPQYVHSLDGKGDCLRLMNDYQQALEWYQKALDINPQYVYSLYGKGECLRLMNDYQQALEWYQKALDINPQYAYSLYGKGECLVQMNHYQQALEWYQKALDINPQYVQSLNGKGNCLALMNDYQQALEWYQKALDINPQYVPSLYGKGECLRLMNDYQQALEWFQKALDINPQNVYSLYGKAYCLLQYGQYGDAQLLLENAYYLSPDNKIIDELLLQCINSLNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X1K0</accession>
    <name>A0A8S1X1K0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1060197</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X1K0"/>
    <dbReference type="OrthoDB" id="291654at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8194445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="255" mass="30301" checksum="95B1D9ADA74EC06D" modified="2022-10-12" version="1">MIKDSESVNEIIEKINKGSSVPIQKLQPNFRKKKVATKYQFSSIAKFAIVIQSIFLLFGSLYLASQKVIYRIPMECYLIYNNSLCKIILTGMYVIVFLRIIRNMIKVYKKKEKQSKYFFHSYIFNCDITIQTFRYYLGIKTYILLLFAMQFVIENYLLITVTQIWSNQINENSGTYFMISILMQILIFDITYEYFMIYSGRYGVADCKRSGIVISKQNIFDAICKLKLLSFCYKFYKQTKENYYIYTRKKGIPLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X3W2</accession>
    <name>A0A8S1X3W2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1100103</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000110">
        <property type="protein sequence ID" value="CAD8195721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X3W2"/>
    <dbReference type="OrthoDB" id="292525at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052798">
        <property type="entry name" value="Giardia_VSA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23275">
        <property type="entry name" value="CABRIOLET.-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23275:SF102">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_170, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035779113" evidence="1">
        <location>
            <begin position="16"/>
            <end position="710"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8195721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="710" mass="75574" checksum="DEF492D4FA8BEBEB" modified="2022-10-12" version="1">MRVLFCIALLLVVKGAIIVTCPKNGYGCVTYGANIVIQTTRTNSLCRNGYYWNNQACMPCKNADQVVGGYYTCSAYYEGTSLTCISGYTLINGVCVQIPLGCTAYGLNQNSDAYVCTACTSDYTLKAGVCINNRYCSSWDDNAVCTSCQTGYFLNWDLKFATISANLNNQSDWINTWQTYYKFPGESFCSVCSQTMANCKSCNSAYTCSQCNDGYYWQQSISSTQLTSSNAQFAGSCVSCLTSGNCSTCQGSSCTQCKTGYYPTKDTTNTVILSCNLCPNGCSTCTSSTFCTDCYASFYKYTDNLCYPLNKCASINTQLTTIPTTPKCSSCIAGYALDPSGTGICYQCGNCDSCTIPSGQQTASLQTSCTTCWDTYYGIKDTNGIVTCKPCTQASDSFLRCQGPVENSSLTGVVPTQCQDGYYLYTLPASGTTQAQVVCVPSNQTQQCVTIVATSATQYTCATCTIQTKLYTNSQCLACDAGLTNTLPQGCTACTGSPTNSIACSACAVNYYFSTGTTTTCTACDVNCLQCAAGPTCTKCAITYYVSGGGCTQCGVANCATCNAQTPSSCQVCLDGYFLSSINTGTATTSYCLKCPFECATCSAPGQVCNSCISGYVLQNGGCVTLSSANCAEGYTTSTLTNQQYPTTQNGCAICKYGFYNFEKRCLQSVQPYAGAVYVQKNIGIGSNPGVSNYETIIYSIFLVLMLLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X8B2</accession>
    <name>A0A8S1X8B2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1120132</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000112">
        <property type="protein sequence ID" value="CAD8196446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X8B2"/>
    <dbReference type="OrthoDB" id="296002at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8196446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="277" mass="32866" checksum="B17894874AE210CE" modified="2022-10-12" version="1">MKTTNEPSLLKYRIIEQKNKKIVLEADEDQFTQFANALSIIQKFHDDIKSQFHPSSQPQRRTKNVVSIQAINSDSEKSNNQSFANANPDIKEDQDFRRRIPHFFLSRFKKWAKMMEQDAAYKYLQNVQESKTSKQQRFELGDLQRCFQVQVNEPKESKLCKNLLKDLFISFLQNEATLQIIHYNKISSIEQKHKYISEIKNMIQEMYELKPFDSYLSSEKIKGKRNAQNNNNQSPIKEKQEDLREEFQIPEMHEKYSNESFKKYSNGPSLVKMNSFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XCN3</accession>
    <name>A0A8S1XCN3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1200074</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000120">
        <property type="protein sequence ID" value="CAD8198927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XCN3"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004771">
        <property type="term" value="F:sterol ester esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002168">
        <property type="entry name" value="Lipase_GDXG_HIS_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23025:SF3">
        <property type="entry name" value="HORMONE-SENSITIVE LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23025">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01173">
        <property type="entry name" value="LIPASE_GDXG_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="3">
        <location>
            <begin position="444"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8198927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="684" mass="78639" checksum="DE5ACB9B122286AE" modified="2022-10-12" version="1">MQSIEDQIKSCMSMTNLTQDITLELRTEEQSEQLEQSQQSIQIQQEWNDNQEQFKQTQKQSLKQDMIQSQQQIINKVFNCPIFNQVAISKELYQNLSETYDLLNKLHLKAIDLTKRFNSQANFLPRFFQAIKITFIGILQLEQIGLITNDKEIISKSINEEYNINVNKKRFNEKAVCILLEFVITHILSQISVLFETIEVRREKQELRLLVIYLSKLQTCLEQLPGAANLDINDIFCYDRGHYRWQSLNSITQEILLGSREQISKSYGKVAEGLLLANAMISKGSEFNNEAAKNVMQGFGALYYLVAKKDAKLKADHFMAEPNKNLAFKAWNLGESGFLSKLLPLLFPMITYNKKIYIPYLFKRLVEDNILRQYKENKINHIQNDCGECINTNLPKCPELFKKNKETIPVRILCHYNLQEIQTGSFVQQSLKLIQGEKVFDKIIIHIHGGGFVSMSSRSHQTYTRKWAKNLQVPIFSIDYRKAPENPYPFGLDDCWQAYMFIINYIDKYFNIRPKKVVLVGDSAGGNLVAALTVQIIKSGARIPDGILMAYPALCLDIKQFTPSLLISLNDPLLHHTVLKLCISSYVPQQFNAKLDPLMSPSIASDEILGRFPPTRIVLGTNDPLHDESFRLANKLLKLGKDIKITEYKWMPHGFLQFDVVQGMKESEQTVLDAQNIIQDLLTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XK89</accession>
    <name>A0A8S1XK89_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1280055</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000128">
        <property type="protein sequence ID" value="CAD8201521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="194468at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009190">
        <property type="term" value="P:cyclic nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47455">
        <property type="entry name" value="ADENYLYL CYCLASE BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47455:SF1">
        <property type="entry name" value="GUANYLATE CYCLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Guanylate cyclase" evidence="3">
        <location>
            <begin position="51"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="3">
        <location>
            <begin position="289"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="1009"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1263"/>
            <end position="1283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1300"/>
            <end position="1320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1265"/>
            <end position="1283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8201521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1341" mass="155821" checksum="23236E3EDFF03BF9" modified="2022-10-12" version="1">MINQQNVNLQSNVKVQSILGFIPALVFQHILENQIKGIKPHVPEIQSFKSVIMFADICGFTNLTEQLSKIGPEGSEVIAFAINRYMELLIKSISKSGGDIFKFAGDAIIVVWPPPTGLNDKDEQLKTLLRQGIQSALDIQAKLNDTFILENIKLSVKIGFGVGDINILYVGGVFNRNEFLATGQALIQAFESEHCATKGGQVIISKDVYEMVGQYYNCLKVEDRQSYFVNSNKGAKVQNISNALLIKSNISPQSFESIKSQLIRYIPAALQQYIYVDQEKWSSELRILTIMFINLSIDLSAALTSNGLSHIQQVIETVQKSIYAFQGSLNKILMDDKGSTLIVVFGLPPMAKQDDPARAVMTAITMKSELNAINCGCKIGISTGCTFAGVVGTSGSRREYSIIGDAVNLAARLMQIACKDDQHSILLDVETSKEASYKMNISFYRSVMVKGKADKVNIYYPEINLELNKIRIHNPFFPRLLDNYLFDINNLRLIQRRIQLFQLQNESNVLLQISGNQGSGKSYSVKQCIDLFQENFNFIQIRLCSFLQKEQQYLYAIKIIFQHFLKKIAIILNKSYDIYLFNEIFNQQDQLLQIFDFEPISQIQLFDTQFKDKNDSLETLDIQMIKESIDLLFRKFQSITNYNKQIFVLDDGHNIDEDSLKIIRHLMKHNQFILLIFIFRHDYFEQFQFHEQHQNQIQMIEDGLDSITSKLNDINNSEHISFKNISLNDYQILLNDLFHINKIRCSKNQEPSNSLDKNILHYEYIKNQLIQSNTQEFFLQTIYRKTQGNPLTFITLIEKLLKCNYMIVDNKQQDVVIVNDSLLNILNLDEFIILEAPLNKFRLNSPYLDKVGCLELLILKVASIIGDIFDVQTLNKIQPFKEVIQKENLIKILNSLEELEIIETMELNDLNKYYRFAKPFLREIIYQRLLFSQRRELHKYYAQALQEIPSQFEIDEKLEAQRLEHTWILAEQKWNSQSNIKPRGLELSHKAKRSIIIKSIQTKLIAKTNNIKLGLLKKKSDKNVTWAERYCLMTQKELKYYYSEQDYQRQPQNALATIILSSIYQIVPLNDKEKVNQKYAFEIRTGNWFKKQKLMQRRDFYFSANSDESMEEWTIYIEFMRVKATYDEFVNNFGKIQFPIANFKEFYDMSLGTEINNYQQKQSQNKNSKSSLIQTQKRNTVLSINSFFKQSVVEKKQKQNREELKILISKFLNRSQLLLFSHIFQNSIQNRNHIIIGQNTKAMLNFGQFFIKTISVYSQQQQQQENDTIQTEESPQTRPRASVNISEQLVLEKQAESISDSIRNTKRKQPNEQFYNKRSKSSEFLVQFKENSNLEQQQQQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XLY7</accession>
    <name>A0A8S1XLY7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1290050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000129">
        <property type="protein sequence ID" value="CAD8201837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XLY7"/>
    <dbReference type="OrthoDB" id="312042at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011:SF28">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="63"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="197"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8201837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="418" mass="49341" checksum="80408D992A78FCBB" modified="2022-10-12" version="1">MQPQNWNNAVIQNLQDMIITQADRNIFLRQSQKLKVLLTIPPKERTDEHIKQISVLVQSVKFISRYREKPYYNELCRNLYVKTFQQRKTIFKQGDTGTCFYVILSGLVKIYADEPTCFSGYFKKREVAVLGKGNCFGEIALFFGSQRTATVIAESDCDLLMLDKDVFQKYIQGDSEDLDLQSTNLKDVRRFLNTVQQFSLFTPEEIAQISTKCHKQIHQQQTILLKQGVVPKQLFIIKKGRVRVIKKIEVGYQYYELDELESGTVFGDHACLTDCKSEYTYITSIASEIIHLNGFDLKQYVSQDKLDKYVESIHFYPESETLFKMQIEDLQWKQYKKQIVQNVVNDKLNSRGFDRRMRLPELRYKLLDAPDQQIYQRAFVLQQSCRTPQHKQLFRREIESLRNQISPKRMKRFEQNYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XPA7</accession>
    <name>A0A8S1XPA7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1330018</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000133">
        <property type="protein sequence ID" value="CAD8203079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XPA7"/>
    <dbReference type="OrthoDB" id="331341at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039905">
        <property type="entry name" value="CD2BP2/Lin1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13138:SF3">
        <property type="entry name" value="CD2 ANTIGEN CYTOPLASMIC TAIL-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13138">
        <property type="entry name" value="PROTEIN LIN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="255"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8203079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="321" mass="37859" checksum="9BAF8A0F4A77A870" modified="2022-10-12" version="1">MRKIENYTGEQARAYKKFAQKAKGSRADFEADLNFNEAPSKFVKSNESKQTLKSIKDQIDYFGYEIEPFNMKNERDVGKFDDGGYFVFDKAKDKSVNDAWLDQLDQSNMFQELQEKAQAENSKKIQKQLKEANKNYERRMQKIRGDGFFSSTKFTSKAEAEEFYKNKQQKNEEEEVEVEVEANQKVEEKEKEQQVIQPEEQEVKLEQLRIDQLKEILYELLEENENANQAMKRVRQKLANNQKKVFKKNVRKDNSQQNDNQNQKVQENNNEAKDEAKQTLDILVDICTIIVKKSNDPSIYNIKKEELIPQKVSRLEEDDIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XPH0</accession>
    <name>A0A8S1XPH0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T1320138</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000132">
        <property type="protein sequence ID" value="CAD8202970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XPH0"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14003">
        <property type="entry name" value="STKc_AMPK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14335">
        <property type="entry name" value="UBA_SnRK1_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000740">
        <property type="entry name" value="SNF1-related protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF82">
        <property type="entry name" value="KP78A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="23"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="9">
        <location>
            <begin position="296"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="383"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="455"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="514"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="577"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="384"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="521"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="577"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="594"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8202970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="620" mass="71066" checksum="2FB48828E19AD663" modified="2022-10-12" version="1">MNESDKENKNGIRLNNRRIIGNYQLIKTLGEGTFGKVKLAVHLKSQEKVAIKILEKERIVEVADVERVSREIHILKLIRHKHVIQLYEIIETPKHIFIVMEFANGGELFEYIVKHQRLQEIEACKFYQQLISGIEYLHKLCIVHRDLKPENLLLDFNNSIKIVDFGLGNTYKKGELLKTACGSPCYAAPEMIAGQKYDCLMVDIWSSGVILFASICGYLPFEDQNTSALYKKILNGEYQIPKFVSNEGANFIKAVLTTDPKKRITVEQMKAHPWFNLYQTQSKISPGIIVGYNRMPIDDSVVDSLSQQGYDKEYIIKCLDANKHNDVTTAYYLALKRNLINGIQSKADINSSVFEESLLEPKQRPKKPPMSNIVATSIFKQNRSGSVQQNGQTNNNSQNRGKSVPQAISDDQTQMLVNYAHKMKYQPINPQQKFAIPTDVTQIISLQSVDETLERPKSISYHVPKKTPTNHQTSEHNKSKTGVQRDSVSPQSKSKQKKSLQSKQVNSSFEYVSINNYSTKQPEERKKSNSKHTTKDLFDLTNYQKKLMIDCNQKPTSQLWQLQNNFRQNSRLYEIPTSTKTSNSVNKRSKVIKQENNNKSTNQKSTLNNSFNFDKKSYYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XSD6</accession>
    <name>A0A8S1XSD6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1340084</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000134">
        <property type="protein sequence ID" value="CAD8203608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XSD6"/>
    <dbReference type="OrthoDB" id="63989at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="119"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8203608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="448" mass="51904" checksum="E5423B67FB370EB5" modified="2022-10-12" version="1">MNQSVGENQQSQPSINYGNVKYSFKCLRRHLFKDKVYHVHALQNMITIGEHFNDPNPKYKLELNLKNTVFWKQENGQNKAFGIRYKKNVKYFEASSEQLTKFRQAISCLIGFHSLDEHYKLEKVIGQGSFSKVHKVYRMHDDKAFAMKQVSSKQKNDKENMQLLENEIGILHSLNNESILKIYEVFRIEEHQYGIIVEFIDGICLATLIEQLKKHQNKLKEYDIKIMLQAILKALVIIHQEQVIHRDIKPQNIMISQSSFRNVKIIDFGLSIKNQLQYNRCGTPGYMAPEIVNMKKDQQKAWTSLCDIFSLGVVFFKLLSKGISCFQGQTSDQVLANNKKCQIDWSIVQQYNYSKNCISLLKAMLAKDPDERITAYQALQHPFFADLPPVLSTDFAGNSMTFKSKCIMNQTIDKPNLESINSLNDLSGLQKQQIYVRDLRPSIISRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XT11</accession>
    <name>A0A8S1XT11_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1340116</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000134">
        <property type="protein sequence ID" value="CAD8203672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XT11"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8203672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="77" mass="9271" checksum="A19AFA0FBAD39F87" modified="2022-10-12" version="1">MYIFYKQYKQIRGGGLYNKNCQKHGKWTLLSDNFFMYNLITYIGSFQDGEKVGQWDIMKIQIQDDNLIFEKIGQKIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XUH6</accession>
    <name>A0A8S1XUH6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1340117</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000134">
        <property type="protein sequence ID" value="CAD8203674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XUH6"/>
    <dbReference type="OrthoDB" id="10387684at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8203674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="291" mass="33826" checksum="7620820D375478D2" modified="2022-10-12" version="1">MVSTCDSQIKIWEFQRGVLKLVYSLQGHEKFVQILYIRNLNTYLISCSDQIIKLWKLFNNWQCLETISLGQVNMTCIVTDSTEKLIIFGSADNSIQTWQLNQNNNLSKTCSLMKHSRQVNLLSLNQSETLLVSCGVSCPQDRDQIIVWERTKSQENPFQFKYFVRQNLDIKGPQIKFINNEQFVLISSDNNKVLLFEDCKGELIQNFGKDVELSTIGNSERLFPIQYNIQKKLLYFKIKSCIYILDSNLQLQSFLKSNGENIQGCVTYDGKYLVYWDGKNKGYSIFEISYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XUZ2</accession>
    <name>A0A8S1XUZ2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1390054</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000139">
        <property type="protein sequence ID" value="CAD8204935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XUZ2"/>
    <dbReference type="OrthoDB" id="303271at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8204935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="215" mass="24881" checksum="4753F33CE42AEA86" modified="2022-10-12" version="1">MIQQGNGFRVTNSQSKIKKAGEILASLQNSIKCKSYTPQHRTIKQMTHYPLRTQVNQNTNPLVQPRNVFNRQTSINCQQRPSLLSVITSESNASADEEYEIWDNNQDLRVKKMTMEESKPSVGQFLQLYKARFGGFPGQQQSIKNEEVIKVAVGYASSNKQLFKKYFPKKQIVFEDDIDYSQPYNIEDDHHFQSFNSFFQNKKSNTILKINSSRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y119</accession>
    <name>A0A8S1Y119_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tyrosine-protein phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1400095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000140">
        <property type="protein sequence ID" value="CAD8205324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y119"/>
    <dbReference type="OrthoDB" id="10252009at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46653">
        <property type="entry name" value="SPECIFICITY PROTEIN PHOSPHATASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46653:SF1">
        <property type="entry name" value="SPECIFICITY PROTEIN PHOSPHATASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8205324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="300" mass="35383" checksum="550B5EB325DA7210" modified="2022-10-12" version="1">MKCPTRIIDGLYIGDQSAAHDLEFLITNKIKKIINCAGKQIPNHWESIGIQYLTYGWIENDYQYAIDHSRCFSFINDTLEAGEAVLVHSINALNRSVFVIVVFLMRKFKWSLQKTLQYVQNLKNNPEIKQNVMQQLLNFEKWLLISKLNNNQDSNQNFDEVVARNTYLNSQKPQECEPRNKREVGSKKVQWNKIIISQYIPPYYQIQFKNVLKQELESLNQVQNQNTQLPIKPRRQTIDNWMNKKLSLNQIVDEKKLNLFNIKSDRQQIRPQLRPGTAPQKRLVKNTWQSQSHRVLVKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y159</accession>
    <name>A0A8S1Y159_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1430056</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y159"/>
    <dbReference type="OrthoDB" id="293762at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8206152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="186" mass="21724" checksum="336AB1974CDE5E97" modified="2022-10-12" version="1">MAQLSTNRFSLPIIGQKDAKSKQMPVTRTMRPSLFLNQIDIIPNSLKTEAEATDRSISKPIHTQIESDPLIEVSYHFKLPKLATQRAQKIQQSLPDLPLVNQESRNDSPIDYQRPSKRTRLFLRRQDSKLDVPVLKFQRKRVEFKKSLVVIDLETGIKDRQELNEVKKPLRRIAHQKTRSYQKKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y357</accession>
    <name>A0A8S1Y357_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1430052</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y357"/>
    <dbReference type="OrthoDB" id="305049at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8206144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="175" mass="19834" checksum="D3925A7810A7BBE7" modified="2022-10-12" version="1">MQQQISTRQTNQIRPSMFLNNLDLNSVLLSPKLISSDLEKSPLINQLKINKKRDATKLPVIKEKSAISISNKESLENTLYNNSSSQLTSMKVTFQSGDNIKLPPIQFTSPQNVTKHKKNFTEGNLIKKRVEFRASILVIDFINQVMKKDKIDGSAKPLIRKGMKRQQTKLLPTLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YBI2</accession>
    <name>A0A8S1YBI2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1610089</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000161">
        <property type="protein sequence ID" value="CAD8210971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YBI2"/>
    <dbReference type="OrthoDB" id="302695at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="92"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8210971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="232" mass="27420" checksum="F6452670C9C207CF" modified="2022-10-12" version="1">MQDQFLNPIYLSPHQLVDLFNNSQPDIKFEDESKIVSKNNLDSPPEQTQEFSFSEFQQINSNNSIPDNNIDTTNFILTKDQYVQLISQIELNKRLNQQYHQLLKENQEQLQKRKQLEDQNSEMLDSNFQIKREIQEIKQKLFITQSALQLRKDKQIIELKSQLKELQHKLSEKRDTLQSTTYTENSTVESEKSNSQYCNTSRLVRVDQSSSHHQSIKPYKSRNNSLIKLIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YDF5</accession>
    <name>A0A8S1YDF5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1560085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000156">
        <property type="protein sequence ID" value="CAD8209862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YDF5"/>
    <dbReference type="OrthoDB" id="10679515at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8209862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="279" mass="34013" checksum="AF040929E9799C42" modified="2022-10-12" version="1">MCINKSKYIPGLQVRQQFVKIQHVAQLISQKFQHALVYQFKKKPEKHVKQFFVVVQHVLQFGSQWFNKLSNQYRNLVNINYQINLNIIQLDKLSNEQQQLNKYHNQYHKLVDKFLLLNLNKFPSCKIHNQLLFLNKQHNQHHNLNHKHLLINLNIAQKNNLNKNQYKFSKLHNLHHKWNRKNKLKYLNTNQFHMLNNEQLLFHKLHNLNHIKININCLTHLNTYLNCKLNNKLLYLDKQHNLYHNQINIDYSINLNTLNHNKKDNQQQLFRKFRNLNHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YEY4</accession>
    <name>A0A8S1YEY4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1680001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000168">
        <property type="protein sequence ID" value="CAD8212370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YEY4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="370"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="405"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8212370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="425" mass="50479" checksum="DC501DA968609BE5" modified="2022-10-12" version="1">MDQQIAAYSIKIIVKYHYIKVSVGSKFIMMLECSDYKAENVFANQNQNACAKRVLKGVLQARKVMYQINEVFFKIGQKFLMFQKIIVSWIQTRGICIEKECENLPFTQDYECKTYLRMSLIYLCYTNKWNSLCKKVVSCHTAQDFLKIYQQSQDYDNLLDCVRIKRIQQCVLRKIKDVKISQACDAYDFEIGFYLIEYQYFIWHNDNSKQKQCSHAPIDYRLTYCHQYGNCIVQQKDDLKWHLIHVKRYWKNIFTNYNIYRCIQLGASAYYLIALISKYYQNCQKLKNHTIKIIIYAQRQVNFAQEISILQVVLTIFGKILERKSIVDSSNTDLLVEFGKLDQLIIFLNQIKFMIKLFNSKYKEQGINKIQLTNLAVGIISLTIAGFLFVFFLNLPKDEQRKSPYQFISFYQIMIILQILFSKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YGC1</accession>
    <name>A0A8S1YGC1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1730003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000173">
        <property type="protein sequence ID" value="CAD8213030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="310156at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="226" mass="26590" checksum="E36B71D643B21E3A" modified="2022-10-12" version="1" fragment="single">MPNQMNYSPFILRGGGCGMTKTKIALQTQNNSIQTDAYDFFLSFNHYIYIIESKANVAANEQESQEIMIAIRWFIFQEENIYKINKNVQSVKKSYNLILDGIRRLLISCQVYIRSDSFKCLYILQITTSLSKVIFSFHLMNDERFMKCETQNYFLDICDELQQQIKIERNDLIQNQIELFLFLTKTSFEIAPNNSKEKEEILKGSLKALTLSYLNHYLREPAYFTX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YH66</accession>
    <name>A0A8S1YH66_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">STOP protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1710003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM154 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000171">
        <property type="protein sequence ID" value="CAD8212738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YH66"/>
    <dbReference type="OrthoDB" id="365640at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033336">
        <property type="entry name" value="SAXO1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31516">
        <property type="entry name" value="STABILIZER OF AXONEMAL MICROTUBULES 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31516:SF17">
        <property type="entry name" value="STABILIZER OF AXONEMAL MICROTUBULES 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8212738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="280" mass="32081" checksum="9D486ED0F93E9200" modified="2022-10-12" version="1">MSEQDYQNNSRYSTLQSNNYQSQLLSTHFGKLLDKNEAFFNHLKGDCICGRCICGQCKCPKMLLSNDFKKGQESLYQRNFIAHGKQKQIPLIDHNVTYKSQLPMDINTIQRSTYQAHADAKPAESLKPEQKVFLIPFSSSSAYRLDYTGGGMSSLKINPPHHPTVVDIPMTTHSTYQDNFYKKPIPREEFLTTANKSSFKSPISPELPFIHQSTNSAFYKPYQTGKLNRIDPEQNVRMIPAFDGQFNSTTQKDFDDKNPKKCPSRVFLNSLYKQKLQQIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YN55</accession>
    <name>A0A8S1YN55_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1900003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000190">
        <property type="protein sequence ID" value="CAD8213917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YN55"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="143" mass="17120" checksum="6D40BEA02551E341" modified="2022-10-12" version="1">MLEISFMGNQQNLRDQKQRSYFQKESQLSRLKKGMWSYRVNLITFIEIQNQIGIQLKMDQPQNPQSSIGHLQKESQRQKIVIVFQYLKNRFLILSDCIKIKVSLLTISLSLERMLNVTIKEFRKKGRKLDLRTLKQDKFFNPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RWE7</accession>
    <name>A0A8S1RWE7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F5/8 type C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0010268</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RWE7"/>
    <dbReference type="OrthoDB" id="313433at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40682:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_48, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40682">
        <property type="entry name" value="F5/8 TYPE C DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="172"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="220"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="345"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="418"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="526"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="276"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="223"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="418"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="441"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="462"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="486"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="541"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8131752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="554" mass="65261" checksum="937B58055351A6F4" modified="2022-10-12" version="1">MEHQNYCTTENGVRVCNVSSTLPNCAAENILIQERKALWMSLAGLPQYITLDLSQVQERPKFIKCFGFDCWHDYQSNPSVIELMVSLNGENFITWTTLYPELKQGIQLFQIDPLGTRYQFIKIIIKETFGASKTYLNQVYLLEEYAMISTESVTESQQQTENNNSQYKQQFEKEELEHHERSDRMDRFDRIDRNERNEKQDMKINEYHDLAQKIHSMGREVASLKQSHQQGKSSQSQTKQGFHKSNITEEVPDSFQRLTSTERMVIDIQSKNLNEINILKKEVQDWKDKCEQLESQVQRLFKLVTHIKEKQQEQEDSIHKYIQQNQSEVSFDANKKQVYNNGQVTPQQNQQSQQMTPTQQPQQQQHQINKFEEVIDKKISELSGNFQTLFQQQESKFKNQQKELFKSFKDFIYQEQQQEKYKDYEKKKPVSRGRQKDKSVSKSSSVKSSHSSSSSSIINFIKSKKVYSKSRQQSKSHLKSQPRSRSPMDSKENDRYENNRLSPKQRKVASLLGILQEKLQRRAKKIEQLNLSQRKTRKRSSIQNSMDKSNSSDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RWZ1</accession>
    <name>A0A8S1RWZ1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0010373</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RWZ1"/>
    <dbReference type="OrthoDB" id="305605at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8131962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="116" mass="14021" checksum="916B7E4EC83C9627" modified="2022-10-12" version="1">MQQSHFYYDMFKIISLLFQQDLINKQEKDELKFMVLESQSELMFQLQAIYRSSVKGKQLHMVHAIKNYIDSHRQGSPRNSSPRSSRYLEASRMWSIYEENEEEERDNEIKLIGIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RY92</accession>
    <name>A0A8S1RY92_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0010608</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RY92"/>
    <dbReference type="OrthoDB" id="302303at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8132432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="307" mass="36367" checksum="A5EACA421095DA15" modified="2022-10-12" version="1">MNHVFSQHYDLSHKQVGTNKKIKKMKSKAINQNTSNHQNVSPSQNDEVKYLLELTCSKDVEKKQQNEHSRPSKRSSCLMGYLESDVFNNSFKIVSIQQQKHEIIVKCIPFKEANLLDIKKDFPFYINLDQVSTNIYSYDTQRTLECVQQKEVPLEKFEFIINPNQNIIGCLRIVNDSWLKMFGINQDMMIHNLLRNQSFPFGWGLENQFIQNQISNTFSNKKLRVQLVCYNGSKFNAEIQVRSEIETNSKNQFIQRYLIQYFINRENLSLSQVEQNFQMYFQLKDLTQELMEKFIEQTNKQCQIKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S047</accession>
    <name>A0A8S1S047_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0020152</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8132950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S047"/>
    <dbReference type="OrthoDB" id="298018at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8132950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="262" mass="30767" checksum="D0E92246D9DC33E6" modified="2022-10-12" version="1">MQNSKKKQIFCNQFELFEDKKFKQIPSLKLVKVEQTNHKEQIFNESSAEDIFEQMGNKESNQDQLKKVNKESKVNKNDDWKSYYNSRLKEIKLERPELNHNQLTQLISEEWKMIKKQFKKVPQPTNNIDDVQNKKKIKQNKDNSKFCQEIIYEKQESDGELDFNPFECAKFIENLRSKKFKLVINDMTYELPGTAIIEAVDNDSVLIYLTENNNNNNNTNTAYGTPNEKVNANLEEIGSDSEQSICKGNDDFILQNNIRSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S6M5</accession>
    <name>A0A8S1S6M5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0040187</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S6M5"/>
    <dbReference type="OrthoDB" id="290643at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8135365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="338" mass="39429" checksum="103979A600EEE971" modified="2022-10-12" version="1">MKIVMRSSKLEQLMKFKGKHDISCQEFKQLIMDTRQQFTQLNGEQLNCVVDLMKHDQLVDDDNTDYLLTAIKNVEDQQIKYQLLDKIILPTSNFSKEQVKQIEQIIENCQQYAMGLSLMGKMVAFYDYQNIQIMNKFVKEFDVTKTNAEQLGAACIGLALGQYKDKQLWDDIAQHCLKLDKDFHSYMRIATGMGLTNNGNVEFWNDFIEQFRKQLKTINEDLFTQSLQVISQQNVVSLELCEAEIEYYIINSHIRIENMMSIYVSMARKQLGSQQLYSKLQQDIYEMQLSGDDLILYVIELMQVKDRLPNQTLNVLVAKAYSESQNQELIDKLTNLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SG19</accession>
    <name>A0A8S1SG19_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0070307</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SG19"/>
    <dbReference type="OrthoDB" id="301873at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="129"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8138776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="570" mass="68138" checksum="5F3A68A4A4CB586E" modified="2022-10-12" version="1">MSQEIYVSDDGKLKLLNGREENMRLVNSHTRQVFTQISDFQYNIQQPVIEYSSQRIRTQDNTQILQTQQLPPPKISNQQVLRQNVSTLILEHIVFNKIVNKLHLFLFQKKKQKLHGDWNVNIFIQLLLIQREERKQKLLKRNNTRIEQLESQLQQLRRENDQLKNQMMQMRNNYESQLDQLRGDVTLHSANAADAASLQAEFFAMRTQLEDQIAGLRRQISDLELQLSQSQQENDRLKQLLQNRDSEIQQLRLQISRLQTNAQDTSQLRDLENQLRLSKDESSKLERLLQQANQDIQSQKNKCSVIEQESYTKNNELIMQIRDYENKLALISTDIERLQIQIRNNDTELNEWRMRYTELESQGTTVIQEKVTYLSQEVEVWKSKFIKTNHEYNKCKEELTMCQAELESLKKSGSRKEVVFTQKLLHQGLVILQLQLDQLDHNLKLKELVNRNDSFILSKYNQNHNTQPFHLCLLQNNKIIKQGTRVNVFLVEEIRAIFFESLYQSSMKDQASFISRRLRERFGCYWFVFMWDERRENANPFYTNNMDLVFQFKWLDWHVLIYGIDPKMYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SG61</accession>
    <name>A0A8S1SG61_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0080057</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SG61"/>
    <dbReference type="OrthoDB" id="294019at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8139355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="149" mass="18357" checksum="8C64975D44115441" modified="2022-10-12" version="1">MRTKSCLPEISFKDYCQKYLNDSFITSERQTKNKFKLDEMKSVQLGKEKQLEFQNYIKTAIDQTKQVRIEATKEATKTFGNPKQWLRAQKEYFKQPEEKHKTYIECYQFQMPTQINFNKWKYKERLEQIRYRSYSVRKTPNSQRRQYIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SHV1</accession>
    <name>A0A8S1SHV1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ER lumen protein-retaining receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0070057</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SHV1"/>
    <dbReference type="OrthoDB" id="7694678at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046923">
        <property type="term" value="F:ER retention sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006621">
        <property type="term" value="P:protein retention in ER lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000133">
        <property type="entry name" value="ER_ret_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10585">
        <property type="entry name" value="ER LUMEN PROTEIN RETAINING RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00810">
        <property type="entry name" value="ER_lumen_recept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="66"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8138322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="216" mass="25178" checksum="C4B9980A32C23156" modified="2022-10-12" version="1">MFNPFRYIADFLHLVSFLILILKIRKTRNCLGLSYRTQEIYLAVFCVRYIDLFMYFVSVYNTSMKLLYISSTVFIIYLMKFKKPYCLSYDQASDDFPHYKFIYTGAGVLALLVHTDLAPFELGWSYSIWLEALAIIPQLHMLQKIKDVENITSNYVGALGVYRFFYIVSWIYKYSLTGDVCWTSLLGGIVQTILYADFLYYYIISLKSGKGMSLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SPR8</accession>
    <name>A0A8S1SPR8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0100074</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SPR8"/>
    <dbReference type="OrthoDB" id="284447at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8141359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="128" mass="14759" checksum="B6BBE5BCDF51632F" modified="2022-10-12" version="1">MKQQWIGCFLRILIQVDFIIKIIKMEREERFLEICKGIRSLDGESVNSTIEFIETSCAKEQRDIVRKLLRKGYSNAQIYSEVNRVFGTHSILKVYCWDVQDPKKYLGMGVVGLGIGFGIAVLKRAIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SSY8</accession>
    <name>A0A8S1SSY8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0100208</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SSY8"/>
    <dbReference type="OrthoDB" id="306880at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8141592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="452" mass="54246" checksum="1637EA868AE5D2F7" modified="2022-10-12" version="1">MKQYQSQPQKSPSNREKSNTVFTQMDSVENGCLNENKQHDQYFNQIIQMLQKRQEFQEQEYRIKVASLQKRIKYLIQISSQLTQQNTQLKLQYSDLVKEYDIMKDRIINYEGIMKTKNSDYQLSLDEVTKLKTEREKLIGLANKYHERSKIRQKQIIDQENTITILQKQVNDLQKDNLILVSEKQNLLLQFQQNPQFANQQQNILQQEIVTKTQQNIDLSNKNEIQQIIKRFLNDYCIHLTYINNETNQLIVLLKEYHDINLDINNQSKYNMLIKQFDSLINKYNQMLEQQIKFQYQFNISTQDTLLLNNNNNQNFYNNNNNANNTILSSKRNSYNNFYQNNVKQDILKTSKFIKIQQINMFLTQIVESFIIYIFRVRNLNHQILQSDPSHSEQKTTLKKVIIMIIAIIRMQKKKKYLDYKPMVIELPIEETMYQLLEIISTQQQLIEKFQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1STN7</accession>
    <name>A0A8S1STN7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0100356</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1STN7"/>
    <dbReference type="OrthoDB" id="278280at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032378">
        <property type="entry name" value="ZC3H15/TMA46_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12681:SF0">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12681">
        <property type="entry name" value="ZINC FINGER-CONTAINING PROTEIN P48ZNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16543">
        <property type="entry name" value="DFRP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="92"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="167"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="92"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="167"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="257"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="51"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="257"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="297"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="333"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8141884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="354" mass="41939" checksum="0862E028936F9CCE" modified="2022-10-12" version="1">MSKKAEKKKQEKIIEDRTFGLKNKNKSKQVQNFCKGVAQQVKHNGVSMSKLQAEEYEKKKLEKQLEEDEKLIQSLYKTVEQARDEESEEEVDPKSILCEYYKQGLCQKGKKCIYSHDMSLEQKTAILDLYTDQRQQLTDEWDTCQTWDEKTLKDVIEANEKTYKSQIPSAKVCDFFLDALEKGKYGWKWVCPNGMTCHYKHCLPQGYVFRRKEELKQQFDGDIEEEIDEEIQKLQKGGTKITKEVFEKWKIERAEKKKQEAEKQKQEEQKKKGAKQTGGNNQMTGRALFVYDPSLFVDDEEAENQYEREEQIEENEEEDEQQDNENKQKLYEGDDNQQQDQSEDEDQQFKQQQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SVZ2</accession>
    <name>A0A8S1SVZ2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2HC/C3H-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0130149</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SVZ2"/>
    <dbReference type="OrthoDB" id="265955at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026319">
        <property type="entry name" value="ZC2HC1A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049899">
        <property type="entry name" value="Znf_C2HC_C3H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13555">
        <property type="entry name" value="C2H2 ZINC FINGER CGI-62-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13555:SF68">
        <property type="entry name" value="ZINC FINGER PROTEIN 474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13913">
        <property type="entry name" value="zf-C2HC_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52027">
        <property type="entry name" value="ZF_C2HC_C3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2HC/C3H-type" evidence="7">
        <location>
            <begin position="214"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="369"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="525"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="369"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="387"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="410"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="454"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS52027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8144280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="544" mass="64376" checksum="6074B6EB9CD1BBFA" modified="2022-10-12" version="1">MNNEQFEQLEDEINIQAQKRRIGNVLFYVRKKQNVNLILQLILIQIREQEKMYQNFDLIIQCIILQFKFKEQQLSVELINLTQFIKRCQRSNYQQIKLNNLFKNQRNKIENLFMKYINQKFKKIKINKNQNKNQLIKKLKMFQVLTFKHKINKKEIIIIYLIIIQNPLSQKDLQIYNKISIYLIIICRYEKTNQKTNDNNLNYLNGGGFAVRPKSLLCTICGREFGTASLEIHQKTCIKKYQNDLINMDPGHRQQMPTTQQVLQKLNQEKQVRQQGGTKIGQKSQQFLQEQPQQVMALVGCRKCGRRFNPDRIRKHESVCIGPEPDIQKIKEQQQEQNKRVAKYLRPKQTGKWKQEHLEFQQALREMRKVAQQEKAEGRGNPFGHQQYGQSYGMSNKQQPTKYGKTNQYQQNFHNQPQSKQQQQYKQTHSQQQQYQQQQYNQKGYSQSVKSTHKQPNLEQQQQTKIIQKSPISQMPQKQYQQPTKSSYNDVGYGRPACNVGGGASYQIAHSNVSTFSLFTQDGKPKNYDNYQQNNPYAKKNGLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SW96</accession>
    <name>A0A8S1SW96_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0120348</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SW96"/>
    <dbReference type="OrthoDB" id="299319at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8143676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="76" mass="9248" checksum="3AD0835A34AC4989" modified="2022-10-12" version="1">MRPIPWYVRLGIDVSIFTIPILMLASYPRLGTHFLNNRRRKYRRRNFDEIQYRNDSTLSSEEVISFRQRLGFQDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T2F5</accession>
    <name>A0A8S1T2F5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0160008</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8146700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T2F5"/>
    <dbReference type="OrthoDB" id="532484at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029147">
        <property type="entry name" value="CFAP77"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28617">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28617:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14825">
        <property type="entry name" value="CFAP77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8146700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="216" mass="24974" checksum="5C4EE91EF70E242E" modified="2022-10-12" version="1">MYTTPPFSHNYSNPVLMKDDVGKPKPSTYNLPKQDFVYGLPLIRDKEGAKEVTMTWKFHQESQDRIPNRDFAELNKMSVFNGSLNAHDMYKFRQTHDARLQVKKGTNIQAIELPEEEFRYGRKNRPSTPMKLVMGNSYGIEAASMTLDKYYKRAGSQESKMTNTIVRPNKASQLFHESNHKKLAVIKGTEKKEPFKMEKFKTVSARTDTNLISKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T656</accession>
    <name>A0A8S1T656_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0170239</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T656"/>
    <dbReference type="OrthoDB" id="1926878at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049945">
        <property type="entry name" value="AAA_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050311">
        <property type="entry name" value="ORC1/CDC6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763:SF26">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13401">
        <property type="entry name" value="AAA_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="58"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8147980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="386" mass="45417" checksum="0E456694F4DE5897" modified="2022-10-12" version="1">MKKKRQSLQHDLQNNKKQPSSYDDLDNTQSNVTLLIKRMKFREIEEYEIQQFITSHNEQKFLMITGQPGCGKTMLLTKCIKQWQNNYTTIYINAMQCKNYLEFLNICKKQLNIKNSSGKQQPRQIIMDKLKELNTIIIIDEFDNLFKVSEKEAFDLFSLSKYAIIIGISNDIEFLQTQSVRYKFQLPLYKNLILKPYTNQQLQELVLDILKQMEILKYDENAIKLLTIRAYNDKGGDMRNILDIIKRTLRDNNEITTDAVNKEIGMSVIDNKMKSVVTTLTLHQQIILLGITALLKRDSVQLDIDLQDLIRKVNEIKYKMSLPLSVDFEEDLNLLKDYNLLTIKEVKQQKMGFKYTIKKIQCKYTAEELKYQLSDMDAFKNIIEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T8Z3</accession>
    <name>A0A8S1T8Z3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0170037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T8Z3"/>
    <dbReference type="OrthoDB" id="290466at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011:SF28">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="45"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="178"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8147584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="507" mass="59310" checksum="006744C83325ACCD" modified="2022-10-12" version="1">MTSFSNFDEKTSLINLCVKTLQIAPSRRTQSDLALLLQLVEAITFFKNLQSQNPDIVLKCMTVLSYKTVKKEEILFHVGDSGSLFYIILKGSVGVYILLPSPEDFKKFELKEVNILKAGNSFGELALLNDNAKRTATIIAKEDCMFAVMEKHHFKTILGAQEQQKVQDRISFLCSFPFLQSWSFREIKTISYHFEPIQVTLNQAVIKQNDYCTNFYIVREGKCVLGPGEFFGEEAFLMDDGKTFILKLFNIFQVRWARVKCSIICRSQCGQIFRISREDIIKRFWDEKTQTVFLSLLQSIHQFRLNKMSYYDQIADDKLKQLNGQEGIDQFVKPMLKVKVRNNCALIRKETKQEKSDQEFQFYKKQEKKAFEQFKKLFKDRNCSYAQVKTFFDQKTIGSQNYIDRDSYFKLFFRFPSQLKNSEISQSLNQPQPSTARTQYRLTTQPKEENEKSPQFSTKQRTLLILSKLKLNEVLPKSKTIHNNNSPLNIQRLELRRFGTQSYLQMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TC39</accession>
    <name>A0A8S1TC39_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0190099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TC39"/>
    <dbReference type="OrthoDB" id="283883at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000814">
        <property type="term" value="C:ESCRT II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016689">
        <property type="entry name" value="ESCRT-2_cplx_Snf8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040608">
        <property type="entry name" value="Snf8/Vps36"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12806">
        <property type="entry name" value="EAP30 SUBUNIT OF ELL COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12806:SF0">
        <property type="entry name" value="VACUOLAR-SORTING PROTEIN SNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04157">
        <property type="entry name" value="EAP30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8149418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="248" mass="29248" checksum="08E16E7967E7AF4C" modified="2022-10-12" version="1">MIYTGKKQRAAEKETKENKLGQLQKSQAKQVEQLTLIYDVFQKNLHQFAGKYRDEIQKNPDFREKFNELCDKMDVNPMMSKKTIWSDLGFGDFYNELAIKILDICAKKYSIYGGITKITDIIDEFRKVYGMKVSKSDIERAINTVSSLGGCHIHNKYVYTVPIEMSPDFNLLLEVAEEERFVNEKIMQEKKGWTKERFYQKIQVLQREGLVWADKKTQTKIVDYYFPSLLNTYFDPLRSQMIIQQLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TFX0</accession>
    <name>A0A8S1TFX0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WD-40 repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0200006</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8149932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TFX0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25173">
        <property type="entry name" value="Beta-prop_WDR3_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="399"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="441"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="483"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="525"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="567"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="609"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="651"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="693"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="735"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="777"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="819"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="861"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="903"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="945"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="151"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8149932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1065" mass="118701" checksum="6D884F55CBF8D60B" modified="2022-10-12" version="1">MNCDLHTKNPITLICIAPHKCQCQRKLCVECQYEHKVDQKYTVPIKIFEEMVIKKIKEYKLDESSELKNYRLSFKNMLSQIQSNVNNILEELSESIKLIYDLIEQENISFLSLINQNTNPAESYYTDLEKLVQMVEGTILNDWNDYKNSYILKLEETKNNCEQEIKTFMENSSKEMKKIKLLVNIQLNQENNEVNEMKETLYEILAQCQDIDESIYKVLIEILKKEKITDLLGFLTKTYNQKLYESFIIQHENVNDAQKKIKKITNFLKKISDQPFSKANYSSVTYEEARQDLINSIAENKRIIEFLKFLVHITAFDEKLIQCGSNGLNLLVGLQANLRNQNFENIRIKNTSLIGGNFERCNLSGSEFNNVVISGLNLNQAQLFNCKWKNLKINELNQLICHQKSVNSVSFSPDGVILASGSDDNSICLWDVKTGLKNAQLVGHDRSVYSVCFSSDGTTLASGSDDNFICLWDVKTKLKKAKFVGHDGGVYSVCFSPDGSTLASGSDDNSICLWDVKTGLKKAKFDNHLGWVYSVFFSPDGTTLACGSDDNSICLWDVKTGQLTIKFDGHENWVKSVCFSPDGTTLASGSNDNSIRLWDAKTGQQKAKLDGHDNWVKSVCFSPDGTTLASGSDDNSICLWDVKTGQQKFKFDGHYGWVNSVCFSPDGITLASGSYDYSIRLWDVQIGQQQSKLDGHSDDVNSICFSSDCTSLASGSDDNSICLWDVKTGQQKAKFDGHDGLVYSVCFSPDGTTLASGSGDNSICLWNVTTGEQKAKLDGHDEPVVSVSYSPDGLTLASGSADQSIRLWDVQTGQQKAKLDGHNEPVLSVCFSPDGTTLASGSDDNSIRLWDMTTGEQIAKLDGHDEPVVSVSYSPDGITLASGGYDNYIRLWVVNTGQQKAKLGGHSDCVRSVCFTPDGTTLVSSSNDNSIRLWDVNTGQQKAKLEDHTQPVSSVCISHDGTTLASGSYDNSIRLWNVCTPKAVQLSNNSYEEILLSQQTTAFLNPFYDNSKTYITILHITQNPYLHAQGALIYKGEFINYYGLDLKSLFKSKGSSIIEQEQQTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TG30</accession>
    <name>A0A8S1TG30_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">STOP protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0220326</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM154 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8152195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TG30"/>
    <dbReference type="OrthoDB" id="365640at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033336">
        <property type="entry name" value="SAXO1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31516">
        <property type="entry name" value="STABILIZER OF AXONEMAL MICROTUBULES 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31516:SF17">
        <property type="entry name" value="STABILIZER OF AXONEMAL MICROTUBULES 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="240"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8152195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="268" mass="31369" checksum="2DE176A498CE4DE6" modified="2022-10-12" version="1">MNKSPHNGFKTPDKSASKLSQYNGKCVCQICTCGKDKCPPQQLHVNPSHFDTTNRKSYVKWDVKKTTPIVPEQYKINYGTQSFVSSYQRDYTPQKTPERQTRITQETTIPYHRPFVSSTEYNQRYQPYQTLPIQLKPKYFYQENPNKIQASTTYSENFRPQPISNQHQIKQHDSSFTPTKIQFNSSSKYQEDYTAQKGNRAAPIKHNNQSPFGGVNMEKLSSYQKDYQRLDESTYKKPQCPIRMLPVPDRNKSPNKHLMFSAQKNDWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TH08</accession>
    <name>A0A8S1TH08_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0240197</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TH08"/>
    <dbReference type="OrthoDB" id="293207at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8153411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="98" mass="11467" checksum="99F21BEFB9ED8AF6" modified="2022-10-12" version="1">MADEQAEFFKEVLKLISNLRYENKITEDQKSELKKLLMDDEDFVNQLQEEYQVEGLKGIVNIIIGDTISDCSFRDLIDFNQEKRKRSQSLTIQTDSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TH88</accession>
    <name>A0A8S1TH88_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0210178</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TH88"/>
    <dbReference type="OrthoDB" id="294794at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="250"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8151113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="274" mass="31381" checksum="48AA33E58B27E56C" modified="2022-10-12" version="1">MGNSNKAQQNVIHIEFMTAKQKVKKDKALIKNKVDQSYNEHLQDIICIQDLTQYYQVDFRQTILTTDETIHVKKFVDLVVNPNSKTPSNLIQISDFFKQNVKSFQIFFNNQMFSQEDQKYQFNYEPLYIEVIYKPKHWMEMAVEKFKTYSSKIMSALGFISKFAYIAIPLYTSLSALQPFLLPTLKTAKVALKSTQNPQAQNAQSVVKFLINTLKRASFIAAPILSVVSKYIPMSKTIVSGIGLYLGKGMLWNVIKLGITIFCPFLAPLLMIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1THF6</accession>
    <name>A0A8S1THF6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0210213</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1THF6"/>
    <dbReference type="OrthoDB" id="293713at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18870">
        <property type="entry name" value="NUDIX_AcylCoAdiphos_Nudt19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039121">
        <property type="entry name" value="NUDT19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12318:SF0">
        <property type="entry name" value="ACYL-COENZYME A DIPHOSPHATASE NUDT19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12318">
        <property type="entry name" value="TESTOSTERONE-REGULATED PROTEIN RP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="7">
        <location>
            <begin position="311"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8151183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="596" mass="70377" checksum="38840A70241F497E" modified="2022-10-12" version="1">MGIVNNRDVSFFKNGQPLFVNPNTYIKNNLQPNDQITTVNGQQNMVNQPYRQPANTTPTLPQNLQNQSNPQFNQQFNPQIRATNIVTNQEQTQFKNGMLFGINPQFGQQQNFSQIIQPSQFTNPKPQQQVFQQTIPQSQTISPNINLLGQKAPQIYKQDDDIFASQNQQQNHQEEVDIFATEFQFSNYDEQRLNYLKNKNISPGDKQLKFERDNYIFTIDNFGDELIIQKNDQSFYLKLNSSFQMKQKRQIDIQSKDFGMDSTLGYIGCVIQQRQQNIFEIIYGMISKFKIIINQLYLKNFKSIIYNKNEYLQKNASLILVRDDYKVLVLERSKEISFGGSYVFPGGILEEVDDKIAQFDSQLIEQNHQKYYCHQTQGWYDSFLIAAIRETIEETNIQLDYKQLYQKIKPFLRIITPQMIQKRYDAQFFFNLNNYDTLDINKIESTSYEWNTPIGFLEKFIKNQIQFQSPIFLQLLILQQLGSYQNIVNYIDSGDQYKHTYPHVFQFSNKYVFNYPDQNYNLQELMETEQTDYLKSEFSVKYNHIERSDFRFELEGNQKKLTGHIGKFMNSPLALLYGQIINDSQFMRLNIKEINQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TK58</accession>
    <name>A0A8S1TK58_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EFHB C-terminal EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0220386</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8152313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TK58"/>
    <dbReference type="OrthoDB" id="2096280at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057428">
        <property type="entry name" value="EFHB_EF-hand_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040193">
        <property type="entry name" value="EFHC1/EFHC2/EFHB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12086">
        <property type="entry name" value="EF-HAND DOMAIN C-TERMINAL CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12086:SF12">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING FAMILY MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25325">
        <property type="entry name" value="EF-hand_EFHB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EFHB C-terminal EF-hand" evidence="5">
        <location>
            <begin position="340"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8152313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="416" mass="47473" checksum="2042CF9FAA14FBB5" modified="2022-10-12" version="1">MSGKTRDYFGTLKSAGRTVLKEDSAGDCIQPVQGQVPETPPHIKKYRKSYKHQHGCAILHPGLVDAPKPQGNWLYGKKTDPSDKAGDLFKQQPEGIKELVNEINEQKYASHIKEPLGTMPTRNYNWPEESKSDGFAFGQKIPPSEFTAKEVVFPPDAQRDDESARMMYLKSHGNFEAGEQKNREYKWNVNPSEFRFGKKDEREQEQMKKILQHELTQNQYPKTTIISKNQEDWKNYNEDPLGKPKNLAQINSRMPAIFGETKKDEQWTAGQCINGQPTLKEVQPDIDLGKATKYGFRNQTKQGDETRAFGVPTIRNDIIKTGMKSVADPQNYGDEVPAVALLFPEKFSYMGLTDQDFLMLRNKKDIKQIFESIGIKYGIGKFEGVFNRAKEIQNTFDDKVSVKAFQLAVQEMHHID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TLT0</accession>
    <name>A0A8S1TLT0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UDP-N-acetylglucosamine pyrophosphorylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0230211</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDPGP type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TLT0"/>
    <dbReference type="OrthoDB" id="532420at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003977">
        <property type="term" value="F:UDP-N-acetylglucosamine diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039741">
        <property type="entry name" value="UDP-sugar_pyrophosphorylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11952:SF2">
        <property type="entry name" value="LD24639P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11952">
        <property type="entry name" value="UDP- GLUCOSE PYROPHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8152728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="454" mass="52957" checksum="FCC3D4C4BFBE00D2" modified="2022-10-12" version="1">MGICTSQNDAQKLENSEEDYQKLDFNRVSTYQKLENIANNIATQQQIDTIFSSLIQRMIASRQTHILNMLEKMDNKKKQQFMTKLERIDFEFIDSLFHHCYKQNQSDVFSCNVIQSINSQEQNYDLIKKHKVGIVLLCGGRSSRLSDKLLSDIGLPSKKCVVQIMMERLKKVIQIASENKEIVDYPIAIVLSDQNSDKFQQYLKSKRDFGFQSITFLLEKSQPVVDIKGQVVFEYENQVIMTPEGTGTIFIQLNSFINKFPNMEYIHFFGLDNLVGLPLDPIMLNIICKQKADALCKVIETNQILDDRLFYSNKQFKTIKEQDQIIKENQIQMCLNDLYLSVSFLNKLKQNHEKALKLNQRYHSIKRGDNIQFEKHIQDIIEVTDTTILYQTKDYALLIDDPKKAVIQLSNVHKRYLNLEGTQDKDLVEITPQMSYCKREDLKKIENITYPLII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TMH3</accession>
    <name>A0A8S1TMH3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-CH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0230347</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TMH3"/>
    <dbReference type="OrthoDB" id="304293at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8152998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="259" mass="30632" checksum="7AC14FAB121F30CA" modified="2022-10-12" version="1">MTIELRLHSPQLTKTIEIEDHKKETNYRSLQVQKSLNLFSEQHDEQITMIKKKILNQQLSIFNDELINANYQQNSPEHEQNNLQISLNTLRNNDEQSMQSLSNKNCYFCSQDKNLIQICPCSYAHQSCATNYIQTGSIMKRISCQQCKQKYHLKGCIYFDFKKWKEFKFSLFFEFLLMSLVVILVTYSAIKIGEQFNQNTNQISTYTKIILLCTLGFIICVVVISKILQHFKAIKFEVQQYYPRNIEYNIQYLKLLVQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TNR8</accession>
    <name>A0A8S1TNR8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0230329</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TNR8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="328"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="464"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8152962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="773" mass="90808" checksum="31C8BDA138BB34A5" modified="2022-10-12" version="1">MFQSPKNAISTAKFQQKVMNLETNTSQFDATSTIIQSMYLQIDELIQTIQIRNPKQLKQLNNEENTFTYQNQLNMLKADQESTNLQQYYLAIESEEISNALYAHPNNSLHQQAQMKKTIQSIQLKINQIKQTLNVNENDQREFESKAQAIQEKQQNYASFLLKLKEKQQLNDSVHKLLDQLGQQQWLKLRQLFDVDIIKIAIPFEHIIKLQQLLLKESENLIQVGKYKGVLEDKKQFLMEVQLQSPQINLVLSFDQILLNIIHQPKDQFISKQGLLIQIVKCLNILHSNFCCIIQNLTKLNYSQKQFEEQSKELLQVTAQIKTFTSSKTQLLIEIDKMKKQLKDLQSQLQNSLNTIDRDIENEVQNLLSLYLKDKEILMRDIKFKYGKQYHDHMILDVRQEFRKICQGQQFEQTNKIEYAYNRMDEIQIQIIKNKDKIIQLLKQLKEHKLLIPNTKTEESQIDKKEFLLQLQNLKIILLQLENKIQKKSQRNSEQLLQDLQVEINKLKEFIDQNFVFQKQRSDSIDTIKKTQKFEHARSQSQIPIISTLGSEISIPIQSPKNVVLHIQNPQDIFDSTDDPIQIVKKPFSEWNSYQLSFNLNQLKSISGISGQEQSFITQKESSLHGSILEAMSSNRNKKPEIHLIVFLKLKECNKKYDPIKNEDPTKYGYRLYRIDNQFNIYSSEENENMNSYQKLEMRLEVSQLILDDCSQNALKFRMIYEPSMRGIVQDSLALKHLNLQHLPFKLQGNSVIVNGLCLNEQDLIKLQLLFKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TQW5</accession>
    <name>A0A8S1TQW5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0260094</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8154690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TQW5"/>
    <dbReference type="OrthoDB" id="299997at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8154690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="262" mass="29866" checksum="28C1DD6392768BD6" modified="2022-10-12" version="1">MGNQKSLEEKVKSSMVQLHKMGFQLKQNESTISELKNYNPKKGQGTAVRDAILFGFTKILNAMIILKNCEQLDQYKFVHIVLTDGEDNSSSTSISNLSAILNKVKDICRTLIIGIDMKKEQLPYISKECEYYDVRSSEIESLFHRISVSLGLKVTTAVGAIVQNDVGVIAVSQKKELSLNLRRCKYMIMLTIDGSPSMNDGEGWWLWKTTRWSLARKAVKNLMKSLEDEDRICASIFDQNFRNITETGSLKLQFEKLFYKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TUM5</accession>
    <name>A0A8S1TUM5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0270285</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TUM5"/>
    <dbReference type="OrthoDB" id="298552at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8155773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="471" mass="55323" checksum="95A5A55D474A2BCA" modified="2022-10-12" version="1">MNPLQKIYLQALINQSHQESSQQVKQEENSQKQNVQYLQKQQLLQIIQQCQTQNQPIQQVSKPPTLIEVQNQVTSYFQKIILQKQTINLKNVLEVQLPYFMIIMSNLIIYGLENCQADKEIIKDLVSLYKTNPLKISMSCSLFQTVALLFRSEVIIYDQSTKYLANFLLFRDDFCDIFFNALYCYQISQGQAVSQQIGFTQIHQMIKKQQDDIWTAFTFKLLSQQQQKIIKDQFLLQFNRYTQLLNNFFTKSNLIMDFVAQSVKQTAKLHNDQIFQEYLLIMGSFKATTQDFLKKAIQVFFEDTPDLDLLQFSSNTIKIHKQMNCQILFILPMLLQLESQYLDHIIQNGLPEIKVQNSSNYYLKMIEKLKSKCVYNGEEECNCKKCQCIRRNRTSAKESQKKKREALEKIGPLQDEFNKLQKKVKHLETENQYMTTLILEVFKHPSVEKLASQFLEPLTKIIADSESMEEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TVQ0</accession>
    <name>A0A8S1TVQ0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LITAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0280087</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDIP1/LITAF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8156014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TVQ0"/>
    <dbReference type="OrthoDB" id="5599753at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006629">
        <property type="entry name" value="LITAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037519">
        <property type="entry name" value="LITAF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292:SF6">
        <property type="entry name" value="FI16602P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292">
        <property type="entry name" value="LIPOPOLYSACCHARIDE-INDUCED TUMOR NECROSIS FACTOR-ALPHA FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10601">
        <property type="entry name" value="zf-LITAF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00714">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51837">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LITAF" evidence="6">
        <location>
            <begin position="66"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8156014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="151" mass="17271" checksum="AAE825079BDD4259" modified="2022-10-12" version="1">MQYNQINNQQSQNFAYPSYPEQYPNQYPPPQQPYIDNNIIVSDPYNTQQQFGNQAQQPYIINGPIQVQTPIYPSAEGMPFPIPIKCQYCQQIGTTRVQSRIGDGTICASILVLLLCWPLFWLPCCLEDCQDRVHLCQSCGKVVGTKKYELC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TXF0</accession>
    <name>A0A8S1TXF0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0290211</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TXF0"/>
    <dbReference type="OrthoDB" id="294171at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="2">
        <location>
            <begin position="76"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="BZIP" evidence="2">
        <location>
            <begin position="77"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8156860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="331" mass="39383" checksum="DB4D10CC167AD589" modified="2022-10-12" version="1">MNFIDQDDCNIPFDYEDDMIFPPCYENIETPEHSKSTDLEQKFKNDRKERILKKRIEKKHKTKSESNNNLSKEELRKLRNRNSAQQSRDRKKQQFDSLILENQNYQMLLKQKEDQIQLLMEENNQQRLKIQYLEELNQNYKCMNCAQEASHEMIRVNVITKQKMMNYGLLSLLAITCILSIVNNDYEFSPKPISLHQISHQKYDFLAPKQQFSNSTQLAIYNNLAIPEHQFNNQTLFYNCTGNEKECQTFLNIIKAENANNLYFVNDASDQIPATQDHHFDQGENVYLIKIKQDDEDNFMIFRAKCQITESNKLLFERDSYDSFNNNSLQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U0U7</accession>
    <name>A0A8S1U0U7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0310085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8157954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U0U7"/>
    <dbReference type="OrthoDB" id="303519at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8157954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="143" mass="17064" checksum="DCAC34019F5F8402" modified="2022-10-12" version="1">MKNKGSFIINKKSSIKSSKLFLFENLLFEDVPLAAKQAYLECLEKQINDLDVMRKQLNENIQKQEMIKKQQLQFRKEIENIQNDQKLDHNIINQSMLLSQFITEQQKIDTCLIEQSYLKDLEYLQNQQLKTSNQFVPFENEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U2P2</accession>
    <name>A0A8S1U2P2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0300295</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000030">
        <property type="protein sequence ID" value="CAD8157689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U2P2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8157689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="231" mass="28063" checksum="30798CC6DA7F89D1" modified="2022-10-12" version="1">MICQNESQWKYEIIELINSSFRLAQVDSSKTKQNMNYLQQNPIGTMADENLSTRTQCEDLSKTILMLNQMKNTFKQDYKENLKILRQTTIDEKITNFKIDFSTLLFKMEFNQPLFLNEQKYNINMLQSIYRYFLNEELPEELFIIATYHSIIEKSDDRTKKEVEQNLNKIFNELLIETQLIQKQKQISQNVFDQFNKYYKNFINKEKSKYISLRTERRRMRKKQAFQYKQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U2R7</accession>
    <name>A0A8S1U2R7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0310270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="303797at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="997"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1216"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1328"/>
            <end position="1352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1575"/>
            <end position="1598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8158302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1638" mass="193741" checksum="0164515FF53BB4C8" modified="2022-10-12" version="1">MQFFSKLTQYFITIGSNTERRVMYIDYGNMTMIIYYFIQLIGYIYSQFESKSIKYYENDYLAQISQLSSIPFMLIIIHYDPLTIFAYYSVFITISAIFLISLLQILLKPLPFNSPINKFIKFYFQNFQWFFLTPFNECMVGVITCGRQSYLAQHSIIKPSECFSTISPHYLIISFTGMIMISFSGILSVYCFRNYEFLQRGLLRKFSSLNFVTIFLHQLLIILSFWKLMYSDYYIIIHACYNLIMLCILIDVVTNIPFGFTNETVFYSKILFSSLFFGILITIWIFTNKDDGIIFLTYSIFLPIIFAANSAVFSNYFEQQSLKFCEFINYPLSEQPLEYLLLLSHPLTMTQTSICQALKYLNIHCNNCEDLKCPCNQDLSKFVLSNGPFDNEKLYIWIQYQFQKLIKISMRKQYSMENFEQLTIKFSTFLQKYRENSYLSYKSIQDIVINFNRTQKNNKQYPQFFMSLTKKIQSDTKKELEQLNRSKFMVTKMEFKTLTDLNLFFFYENDLMINIKQQVYYCKEFWILYQNGTLKDFNALLLQSKKILNQIEKVQQIYRIFQQKREDSEYENILTFRINLLISISCLDDLNTQLKIADKLQAIQKESLFTSKQQFHILKYINSEAISVASLISFQSFGMIDLKISNNFISFFGYDHNDQITQIDQLLPIQIGQIHDGLVEKFLMVGQSSKIYNTQESFIKNKDNFIENITMCLTISLPLQADIHYFFILSHLLKQHTYEKKNQDIYEKGYLLVDVNFYVFGISENIFKKITQFSQNDIINLDQIQQKLTLFQLIPDLFSKMQEYYKIKQLQNSNLANYEVLFYKHGGSFHIPNKQFIDNYEQKTKNKNINNILTNKIINNNLIEIEKSFNFSNISQCQFSIEYSVVQNLLNPLINGIMSDFLYYKIEIEFQDDSNNSKSIFQILPSYQDIKKEQINSKNKSPNQSQVMLQSYESEDSNNQIHQKSQKASELKSENSLYNQVTMMNEILSCNKMPKPILNLILLFVLQLFIFIVMIIFLAILFNQKSKIQSNCVKINSIDLDFLDGYSQMMSGSRHVIYFRDFYQNLSDQKLMIDKNNIEFSLNNKLLISWNHINLGITRLINIYKIHSQTLNQGSEDDLNISIINSDLKSKIVQQVKDQSTYYQVMFQIFFMSKQTFTQNLSNFLQNSTDPYTTQISRSLVYYNYFDVSQLVQAKLESCKSYNYYINEYIDRITSYFFIALYLIISIIAIIQFIFYFQIKRQISFYLKLFIQIDGQDSNKCIQKFDNIHKILNNQSIVLSKKNYEKIIAQPSVQYDNLRTLNFNNQVNFIQPQKSKEKQIRIDNHISYFYHIFALVLISLIPIVYALAYQLYYNLISVNVTPINDQAIQAQQMRLKFITTINRYDQFLIKSYFESYCKLSQLNSSFKCSANNIYSDNQILQQIDMNETIIQEELTTFQQLDFSNFFFNSINSNQFNSEEKQSILSKDTCLLTNCDMKKDLFQERLFQENLNSYFFQGIIKLHQIVSSLYVQFDLIYKGLNTTEAKLLAIENIMQDNDYLIYILWGLDAIQYQIAQFSQYFVNTSLTILEDFTSNLLVNVLQSGIGMLLMIVTIQILFIQVQVKRQIIAKQLFKIVPLQIIFQKNIQRQLTIFQRKIYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U4S1</accession>
    <name>A0A8S1U4S1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0350023</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lin-54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U4S1"/>
    <dbReference type="OrthoDB" id="6283463at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005172">
        <property type="entry name" value="CRC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028307">
        <property type="entry name" value="Lin-54_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446:SF34">
        <property type="entry name" value="PROTEIN LIN-54 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446">
        <property type="entry name" value="TESMIN/TSO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03638">
        <property type="entry name" value="TCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51634">
        <property type="entry name" value="CRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRC" evidence="4">
        <location>
            <begin position="58"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8160511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="159" mass="18160" checksum="2988F9CC5454934C" modified="2022-10-12" version="1">MRSFQVSKDSSFRILCPSNKIEMEEETVETQIQSEIKNIYRPIKIILPPPNLPKIVTNTKTCNCKKSQCLKQYCDCFANGQVCSENCNCVGCFNNSLNNSQRKEAKLQIQARDPRAFKQAFKGCNCKKSGCQKKYCECFQNNQQCTHQCKCEGCVNCLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U5J9</accession>
    <name>A0A8S1U5J9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0320223</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8158917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U5J9"/>
    <dbReference type="OrthoDB" id="311339at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8158917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="249" mass="29344" checksum="A3A18E915689394F" modified="2022-10-12" version="1">MGCHQAKIKEQKQITESQIKIKIESLKQYAILKKNQDISKILEQEQQLNQLLKSNLRNKDQEYALFSKLSQQIKYLETINFIIVYCQILSDQVLKILRCQGKTNSLQELMIYIESVIYASDKLNLKQAEEFNLYFLFYFKLDVNNLRNVNPKITQMFQSLFPNSYEINDYAKSFANKYGISEIELNQVGHQFILNNIISQQINNSNNFDFSSKNPYENLEINDENKNNNFNLCSISVKHNKKRILDLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U633</accession>
    <name>A0A8S1U633_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0310167</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U633"/>
    <dbReference type="OrthoDB" id="287236at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8158096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="98" mass="11632" checksum="88059BFAEBF15BAB" modified="2022-10-12" version="1">MEVNQNRKYFRVGSRKVQQECNYEIVTRTGSQEEDIIDLGTQLQKKMCLNEDESHFIEEILYTDPCEPEMNFDEKILIHQEVTFNPQQYLNNENSIIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U738</accession>
    <name>A0A8S1U738_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0320338</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8159139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U738"/>
    <dbReference type="OrthoDB" id="312927at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013694">
        <property type="entry name" value="VIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45737">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45737:SF6">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08487">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13768">
        <property type="entry name" value="VWA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51468">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VIT" evidence="2">
        <location>
            <begin position="14"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="1">
        <location>
            <begin position="369"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8159139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="525" mass="60387" checksum="56A524A313DBB6B1" modified="2022-10-12" version="1">MHENESKLSEVNAPPLFICFDVVNKLTISLQSVHYIVNVQPGFAIVEIQQNYNTQHYEVPIELEYMFTIQKDSVVSKMVAELGDIKVFGIVKELEEAKKEYQQGIQAGKTMILGEQDKQITDLKKVKIGCLAPGKSLKITFEYIQPLKVYLNQFWMLQLSPMVDSSYLTIGCLAPGKSLKITFEYIQPLKVYLNQFWMLELSPMVDSSYLTVYQLKNQSVNYPQLYNQIQKSLNFQGLQQNYQQDITININLQKPITFVKSPTHQILINNDDNQFVKENFTKNQIIILDKKYPNNFLPNKKFELLFSSDDVNSPHSFLTHTNNDALQHIKYCATLTLIPKFNEVPLDDAYSSYINGLNLSSETKINRGNYYFFIDRSGSMGGQRIQKAKQSLILFLKSQPKNSLFNVISFGSQPKRMFENSQVYNQKSLNQVISQVNSMEADLGGPDVFSALKNGKDSKSHLETYNAFLLTDGQIIPDPESITAIKKMKNQLYKYYFQLPSKIHQTFRLNVLTLRNKQKYLTNSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U759</accession>
    <name>A0A8S1U759_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">P-type ATPase A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0340057</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8159912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U759"/>
    <dbReference type="OrthoDB" id="10256233at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140358">
        <property type="term" value="F:P-type transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006544">
        <property type="entry name" value="P-type_TPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01657">
        <property type="entry name" value="P-ATPase-V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630:SF8">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="348"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="865"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="906"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="944"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="979"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1018"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="12">
        <location>
            <begin position="220"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8159912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1054" mass="120146" checksum="83B4D0FA34994D69" modified="2022-10-12" version="1">MSYRVNNAEIEGQHPLLKVTPVRFLRIRLILTIIISIMTGCMFALALRWSKKLYNWFLFTPCNVEETGTHYRVDTQNGQQFTLEKQQMNGIYYFTFRLLKYAFDPKTQCYQPIEFETDGKLIKDIIANRNVLTQNLKIQYYGKCQLQIPIQPLPEFLFEHLTGPFNILQYFAVAVWFAQNSITFPILILAFTAIAVIVNYILYRRSRSLLQKLANIHQNVILKSDTLKTVNGSELLPGDYIILQEGQQLNCDCAILQGDVMVNEATLTGENVPIPKTSLPDHSAKFNFETLNQHCLFEGTNIVKVNSTTENVAIVLRTGFSSLRGQYFRNVLFPAPPSQRFYIQAAKFILSFATIIAVVYGFMLIKYIPMEFKTSLLVLRFLDNIVWSIPPSMPIFFNICKTASLVRLSSIGINGSNADKIESAGRIDTCCFDKTGTLTTLGLKAIKVWTHDQSLEQIANYILACCHHLLLINGELQGDPLEIEMLNFIGWKINFEGKSFFSVKKDQQEFEIIKIFDFSSARQMMSVIATDGTNFYLFSKGSPEMINQQSQDKKQVVLDEVKKYASNGFRVLGLGYHLQREELETQLNIVGMFVLENPLKDDTPQVIQILRNSGLDIKVISGDSPLTTIYCAKISGIIDINSEVIILDYNSSKRQIVIIDNDNQTNYDENAIENQNVIDEVIRNHKMTAITGKFLEFISKFLTIVNPNRDTSISMTQNTSSNQIFTENNAIEYDDTKMKEITMKLISKTRVFARQKPEQKKQIVAMLQEMGRQVMMTGDGANDCSAIAQAQVGISFSESDASYTAPFSSKSTSLKCCVQVLAQGKAAIMTIIEVFQYQLSVNGIKFAAVTIMFLEVQNFSEFQTVYVGFISNIPLLIFLCISSPATELAEYIPLDDQFSYQNQIQIYTNIFFAVLGLCINYGILNTTDRFFEYSKPIEKFQREGYLNSIMFLSLMYYFMSFGVSIYISNPFKVKYYKNILLTLWTTLGFVVAIVSFIFPQRATWCDVIDIKEDTFEGFNWVVLTVVIVTSFLGFFFQALLQRYIPSMYKITHKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UD55</accession>
    <name>A0A8S1UD55_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0360307</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UD55"/>
    <dbReference type="OrthoDB" id="284654at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035947609" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8161679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="169" mass="19926" checksum="0EE03FE7B517460D" modified="2022-10-12" version="1">MFDLNIFLYQVFVLLICVQSLCTEITNQQECNTNDQCYWSYWKPSLCQSICNLLADENRCNQAVDCKWQADSSSCFVKKCREGKDQNECNLIVACYWINSICWDSSCIQLQAEEAGKCPIAYCIWDEKRKLCYDKKCSDYDNEYDCQQLSFCTWFKYKCIDNGNKKLDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UEU0</accession>
    <name>A0A8S1UEU0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0370251</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8162202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UEU0"/>
    <dbReference type="OrthoDB" id="291460at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8162202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="305" mass="36576" checksum="11F544FE4352592D" modified="2022-10-12" version="1">MNPINIQIAVIPLDKNIKKKKQNSKNKDLPRNSIVHTKEFMTSFIFPKNINQSQYFRTKENIQFALTTFNENTLGELGINIFSIGYSVSQTEVRQEKMEYHFQARDFELVDISTIFHMLRLMKMDKACTNLNKYLQRYEQIASIPCSTQFEINQVEEFASKYPYECYTYRHERHETVILKYTHNIKFLHLMGITIDMIEIYLRETKTLPCAIRVNNYLQIWNQVFEAVSNDQQIFEMEVQNFNGNIFYVKIKQVQIFIEQEGKLYGYLYWIYLTDTNQNLAIQNYEKQLEQLYPKPKECLYKQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UJE4</accession>
    <name>A0A8S1UJE4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0400113</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8163882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UJE4"/>
    <dbReference type="OrthoDB" id="304653at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035891468" evidence="1">
        <location>
            <begin position="19"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8163882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="76" mass="8015" checksum="D2411FD9E869E2EC" modified="2022-10-12" version="1">MKAFLLVCLIATSMMVNAIEIPKDQVLSGQSDSPSFLHVQTFQACQYCCGGKCCLSPANCVKGVCNNVVAPTCVQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UL65</accession>
    <name>A0A8S1UL65_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trichohyalin-plectin-homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0430057</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8165650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UL65"/>
    <dbReference type="OrthoDB" id="310299at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="87"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="390"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="265"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="459"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="94"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="390"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="400"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="412"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8165650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="511" mass="61871" checksum="01B0551B71FAAFC9" modified="2022-10-12" version="1">MSTVTNESKKKEAIDKALIQQQREQLKQTVLERFIKDFGKNNKNKIQIITQIVNDYFTKTRVTDVTLKTLKQQVQQAIQNAGSTTQSQVEKSIQESQITQQQQAQHVPQQKPPSSQSRKSNQQQQQQQQYNQDVYSEISSKAPKSVYMMEGDEDDEWATLVKFDTELYKKEKELENVRKQEFKKKIKSELDRQINEKQNKKQEDVQDEDAYVKLHHYQLNVYDQREKEKHDNLQSKIYNEKLQRDKQVRDEQQRKKLEQKKEKELDSLLVKKIREELELEQKEQLNRRNKERERFLRMMKENEEYRKKALEDAKLEKEAETQMQQQYINLQNQLEEQRDLERKQREEKMKKVMGMFAEGVVRDQKELIKQEDEKMLRNIITQNEREKIEEEKKKMKQLDQRQQLRSFLNSQIEEKKRRQEEEEELNKRQAEIWKQDLNNYNDHEKKKFDYIKEVNLRHADILKSQIEEKQSKIKKKTNKMNTAELLQNKDKLKVIAQEVPELGEKVKKIEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UQ46</accession>
    <name>A0A8S1UQ46_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0440161</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UQ46"/>
    <dbReference type="OrthoDB" id="435860at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006913">
        <property type="term" value="P:nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027038">
        <property type="entry name" value="RanGap"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113:SF12">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8166464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="510" mass="56781" checksum="1EF78F7686C72705" modified="2022-10-12" version="1">MPQQENQQQKGSSSTFNKILWIIVGVLVAYNIKLYLNRSSSVHFEINGNEITMQNDALYLKDSDLQTLSKKLSENPQTTKLNAQLSINQFSSFRDFFASLEKLNNLNELQLSLGNNPLVNEQDQAKSLSQALSKLTKLHTLSLGLDSIFNNESREIILKGLSKISGLTQLRVSLINSDLSGDGLKSLYPLIELRNLKSLELLLVGSKLKEEEMKHLQTILHKLPKLNKLNINLYANKIQIDGAITLSGGLFQQKQLKEFGIDLYFNNITANGTESLMGVVGSMENLTSLNLGLEFNYITNEGGIQVGNALNKLKNLKDLSVNAATKNFGWDGYEAIVSAIEKLPPLNNLELIIGINKCGSPGAELLKKALFKHDRLKSLKINFLENYIGDAGATFIAEGIQYQKNLQQLSVNMNFNSLSDRGALELARAVKNAKSPKVVDLRVSQNQITDKGIKDIFVLLEQALPKIDQLEVELLNTALTNATRDDIELKYGKIDKLQIRLNTIPPSNWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1URE0</accession>
    <name>A0A8S1URE0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0460262</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8167165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1URE0"/>
    <dbReference type="OrthoDB" id="287504at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8167165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="152" mass="17824" checksum="B14BD75784F3B935" modified="2022-10-12" version="1">MQTQIQYYYPNLNQNGQFDLDFMNEEELFEWLHQRLLILIITKDVQIGVKAIYHWCQKGFECGIPMVNQILIVLAGLQLRINPKMVDDITQLIQDKEYKQKLIDQLSQAQQKQVNLEVSNSPRILKAKIDQKESGLFGNILTALRCWDKKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UUU1</accession>
    <name>A0A8S1UUU1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0480144</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000048">
        <property type="protein sequence ID" value="CAD8168013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UUU1"/>
    <dbReference type="OrthoDB" id="302471at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8168013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="320" mass="38655" checksum="004DC73C9DCCEDD5" modified="2022-10-12" version="1">MSRDIEFDNEWQALLKEIKLQMQIQNVLEDKLLQMITKFGTVRDRVLILISNQQVQEINQNELLLQIYSHLFEIIHYQNINITELELQQKYEIEKIIKQYEEKLMIPKYVPSSLSYINQLDNNQEKNLNLKQITCLTEENIEKQTQQKLYTQDDDQKVFKGFVMYDFKKQYFLMLIRKGFNSDKVWIEERSKIEKFILSRQKKLDITISMSTQFAQYFIEYDSQTKFYFILLSNNRIPHSPQFELLSRIRGFISSESNLQKQRKNEIESKYLCIVNDIIDAQERIYIKAKNRQFNQSIATIIQSKNNIYEEKKYHSLQQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UWZ2</accession>
    <name>A0A8S1UWZ2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0500198</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8169175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UWZ2"/>
    <dbReference type="OrthoDB" id="318955at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="238"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="310"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="361"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8169175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="381" mass="45317" checksum="9EFCB8FEA1811E3A" modified="2022-10-12" version="1">MTALQEQITLQEPSKYNQEEQIGSGNTNTQLKNQKEESQYFYRLLITQLIIIFISTFLLSNKWFFYMKYTPKSKVNFHTYCETYGRCQQTYEYPNQYNNQQDILNNGGSQENTVYTNLYYCSFIMYPLFLILQSVFRNYQLFKKRQRLFHYLSYPLMSLFIQVCTHQISQTRWYIGFDYFRLLLIYLIGNVSFFYLNQKYFKWQLEIKSTAQKFLNYSIIIISLKGILDFISFNSSNYIPHLLLAFYIQVYLLHLNKNLFEQNNKYIIHNQSLLCLTINRLKSLFDVQDVYKQVAETHFVISSKMMKNGIIGIIIALILRDFLFSNFTLFVLSGFLLILVWDTSLVSTSSKLTLKDQYQAASLFFLDILIPIRNIILVLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UZX4</accession>
    <name>A0A8S1UZX4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Calcium-dependent protein kinase 1</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="ORF">PPENT_87.1.T0520201</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cell membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Parasitophorous vacuole membrane</location>
            <topology evidence="24">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="30">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDO010000052">
        <property type="protein sequence ID" value="CAD8170195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UZX4"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020002">
        <property type="term" value="C:host cell plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020005">
        <property type="term" value="C:symbiont-containing vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000398">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000299">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000383">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000828">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 26 27" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17" id="KW-0969">Cilium</keyword>
    <keyword evidence="15" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1032">Host cell membrane</keyword>
    <keyword evidence="16" id="KW-1043">Host membrane</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0519">Myristate</keyword>
    <keyword evidence="12 26 27" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0564">Palmitate</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 27" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="28">
        <location>
            <begin position="26"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="330"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="368"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="401"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="437"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00060437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00068067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="EMBL" id="CAD8170195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="473" mass="53924" checksum="3C4F05E88CB45D3B" modified="2022-10-12" version="1">MDQEVFLKKQWFIMTHEGKIEQFYDINLKKVIGSGTYGSVVKATLKGTKNQRAVKVIPKSKVKNPDRFKKEIDILRQLDHPNIIKLYETFEDQRNVYLVMELCEGGELFDRIMDKGYFSEAEAHEIFLQIMQALNYCHTNGICHRDLKPENFLFLTKADDSPIKVIDFGLSTLFEDPISQKASGNQKVTMKTKAGTPYYISPEVLKGSYDESCDIWSAGVILYILLSGVPPFYGDSDPEILDAVQKGEYTFEIPELKQVSDSAKDLISHMITTPEKRYKASQVIQHKWMKDGSKNTKQLKLNFGQLKNFNGSNKLKKVALTFIASQLNEQEIADLGKLFKQLDKNGDGVLTIDELREGLIGMSDVQAKELGNIIKSIDTDGNGTINYTEFLAATMEKSLYMKEEKLYQAFKMLDLDGSGKIDKHELQTVLGKSEKIIDEKYWDDMIKEADKNGDGEIDYNEFIEMMDKFSLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V3M1</accession>
    <name>A0A8S1V3M1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0560054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000056">
        <property type="protein sequence ID" value="CAD8172038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V3M1"/>
    <dbReference type="OrthoDB" id="303184at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="195"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8172038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="443" mass="53294" checksum="8D6393E3A388BA3A" modified="2022-10-12" version="1">MDDNFKKLIESTQPESVSTIRLCKRLGYRPQDLTYKSIDEFQSVNNELRQVKYDHYKARVLKIVNEINNVRSRNKTTGLSELPSQTNVQFSSIVDTIEEQSQNISSKDRVLQNLIYQELLRQKKFAQYYQQNNKIQDRKTKLPTQSSQQITIQSKPSTQLLKTEQSNKKCETHREYENKDKFFITSNEKFLVKRQQILQQEEIQQEEQIQKLKEKEKKAIERIQQFKSELKQHFQKSNLEEVKNKKEQLDNEQYQMLITKQCEKLQKLRLKQSQLNKPFKQQIQIRNKTQSLVFDHDLQQKIDNKIERVVNLKSQLLSERQKIISEKLKINQDDVNFNKYHNEQLKIKEENKRIGKIQKNHKQNSYQIDLKHQLLEKHKKESVWEQVNRVSHLFSNPVEYDGYRTQMKLLLNKQRETLDPKYQNRIDILYQLLPSQMAEKVLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V3R5</accession>
    <name>A0A8S1V3R5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0530071</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V3R5"/>
    <dbReference type="OrthoDB" id="1668162at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051425">
        <property type="entry name" value="Formin_Homology"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725:SF1">
        <property type="entry name" value="DISHEVELLED ASSOCIATED ACTIVATOR OF MORPHOGENESIS, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725">
        <property type="entry name" value="FORMIN HOMOLOGY 2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FH2" evidence="3">
        <location>
            <begin position="687"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="565"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1076"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="485"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="593"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="616"/>
            <end position="680"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8170462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1132" mass="129121" checksum="C1C87954CA594FC2" modified="2022-10-12" version="1">MGLEQSKLFKRQPCTLQYKYDDHVVELKYDPKQQIGYQKPLRFQEIIQINPALRDLDQDILIQLQFLHEQALKSIIFDQLKKCDDLVLLYQATKFIVNCTSNECLELINHYNIISRITQELQKDLDKQILIIIIIHNIVGKLPSIVLEQKHIEWLFRHMNPYNALNVSLILQILADICVNQDKCIENIIEILKNLKEEAKWRFATEPIFKIMEVQQNIYLIRDACTFINALAETHSDDEMCELIKQQIEQYGLTVIYDDVKLKLKNQEFQIDHCSYQHAMQFLYEQKYLPHYSQVDNFYFNNLYDFDPKKPLIFTYGYFDEIFNTDPDYFELQKNQLQVQLDVYDSLIKPSFNPNKTILERQNPLFISTITKKGSEEGLVGALKGLWQSRGSETTNQQIILNAIKKANSLIEIQELLIKLNDESSGVTILSRLIDLLTKKFDQIMADQKKRDQMRKEREEGYKQQITYLQLEIKQRITQDNQKDFKNLNEKLNLLIEENKRLQDQSQQYSQLKNKFQDMSSQLLEAQKVMAKAQQVSELEAKLEEYASEINRLKTTHTQPQLCKIQAPEGGSEPPPNPSENITSIQTVSQKAAPPPPPPPPPPPPPSVQSTNNSALPPPPPPPPPGGKTGAPPPPPPPPPPGGKNGAPPPPPPPPPPGGKAGGPPPPPPPPGGKAPPLPNAKPAVPTKPKCQPSVPLKGLSWNILKPDQIGNSVFQGMNENEIKFDIKSLEEKFASKPAKQIQQSIGVSDKKKEVQKISLLSGERTKNIELILGKLRMSNEKIKNALLECDKSILTLNVIESLLNVVPTDQERSLYANPEELDKETLQIADLFYLELCQVPAQGDRMQAIKAEFVGMDMCNECRGKLKQLKKGFEFQKNDEAFKLLIKCTLAIGNYVNGDSARGGAFGFKIDAISKAADIKSADGKETLLMSIVQECEQIYEKQGKGQFFSNDRMDLLDFMCRLPVSQMIIDINEIKKLQKLVQNAIKSQSKLPNDRVNRFEEFSQQLLLEITDLSQLLSTCEQLYSDLCIFYCENPKTLQSDVFFQSIQSVWNNCIKSKQQIEKIQQMKIKEEQRNKLDQQKKQQQPITNQGNMHESLPRRVGALQQLQQSLPSDAVNQLRLMKQNKNENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V5A6</accession>
    <name>A0A8S1V5A6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0550098</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V5A6"/>
    <dbReference type="OrthoDB" id="289935at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035793840" evidence="2">
        <location>
            <begin position="22"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="58"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="261"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8171583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="368" mass="41579" checksum="13AE7FBBE8CB836C" modified="2022-10-12" version="1">MFKVAVCALLVLASTAINVQSSIWTSKDQKAFAQIHQSGWGKFILNFAELHLSTGGILSELNSEIEKLVDEMEEELAGVHHEFNRRTDVHNREVARLEQEIQDKERELFNAHDFYDNVLIPQGERFAAQLEQLQENIVQNRRTLEQATVQRANDHETFEAQVVEHNDAIAAIDECLQLLSTLEAPSLAQVKKVQKNLAKIQNSLKKHNQFQIFVKVLLEITVDSNFADQGALRDIVVAFNNLRVELVDSLNQITADEADQVAEFNAQVIQLNQEHAEFQRAVVVKNAEIEANTTKQEQTLELIDELDADLATLNGQLQAENDDYAFATDVYNATVAEYNKEINAANQALELLNQPRFQDYVKSQLKGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V6S4</accession>
    <name>A0A8S1V6S4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0590042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000059">
        <property type="protein sequence ID" value="CAD8173298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V6S4"/>
    <dbReference type="OrthoDB" id="299695at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8173298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="124" mass="14602" checksum="9FB773AEB4CEFB36" modified="2022-10-12" version="1">MRLNTLPDVLFPNPIQHPNYKISFLKKVKEEILNNQFQNEPMRMSRQNMTIRSLKRGSIRVVSASIVTSQNNTQTNFRSGSSKFKMIKEHSQIEEMDETFSRTRTPSQLVRRLKRGLSMFEKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V7U2</accession>
    <name>A0A8S1V7U2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LisH domain-containing protein ARMC9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0580081</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000058">
        <property type="protein sequence ID" value="CAD8172837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V7U2"/>
    <dbReference type="OrthoDB" id="293022at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097542">
        <property type="term" value="C:ciliary tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040369">
        <property type="entry name" value="ARMC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048957">
        <property type="entry name" value="ARMC9_LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14881">
        <property type="entry name" value="LISH DOMAIN-CONTAINING PROTEIN ARMC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14881:SF4">
        <property type="entry name" value="LISH DOMAIN-CONTAINING PROTEIN ARMC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21051">
        <property type="entry name" value="ARMC9_LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8172837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="483" mass="56372" checksum="9006D68F7B075FAB" modified="2022-10-12" version="1">MKSGNSNQVFSQVPDSNEINEYIKEYLKYSGYSNTLECFEAEIKSKQVSSKMSNKQQQQKQLNDDIPRIFQLLKTDNIKTKREINLEKEQKLFNKKYQQILQAGRQIFSVSINLLQLLHSLKETAKNENLSESLENYKIQLGKYHKVIINEGKPEGNELITEQVMHEHKTKLFKNYQDKNVDGMIEVLLSLRVNALQIAPELRKNLVYELIRNDVFNIDATDKFDFIVQLLDINNQSLRHAIASLISVISSTLRGVEYLTYNGGNMIIIEKIIKILKEQENGSVTQRFCLAILQKASIKDTVIPTYVHNEMIQWIISLIQKSINTKIHIFCLDFASATLANIIHTPYTLTYLEKQSRFTHQIMEQFLKFIKEQIQVSVMMHILICLSYLSKENFAKQMEECRFIERISEFVEYYSVINTENEAAEIDKKTILDLCAHMFHPKDTSLDNSETLELNELKTEDRIREYENEQGELIFECFQDEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VAL0</accession>
    <name>A0A8S1VAL0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0600153</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000060">
        <property type="protein sequence ID" value="CAD8173954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VAL0"/>
    <dbReference type="OrthoDB" id="300375at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF98">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="4"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8173954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="460" mass="54256" checksum="D9DEAE907D12F298" modified="2022-10-12" version="1">MQNYEILEKLYESKTTVIHKVRDVKNNNIYAMKEFMGFHNQGLNEIQLIKKCQIPFVIKIKEAFILDDNVIVIMEYADQGNLKQFIGQHQGVFIQERVICKILIQLVFILIYLRENQICYKGLNPENILIHQDQIRIADFGIDNLILHQKPSYRPPELLFHNKFSYKSIMYQLGLILYELTTQRHLFKFELIEQIKAVIHNGGAIVKIPSFYSKQLRFIIKNCLELQESERLDIRQLIHNENFKFILNQKYMTFTFEQQSIFDKWCNVQSQNEQIISIDIPINENENKDRQSNYTAYTQEIKQIHKSTIIETKIQYQKSIHKSNLQRQQDEIMSLRPLSSQTKMTQSTPILKTNNFFGKNNFIIQNNRKIKSSIRRGQQSNQLYSAGNQDTCLKSQDIIDNLIKLKMEQCIETIGVEETVQTLDFLRQGGSINEFLSKYQDLKHQCIAKHLQDIISFSQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VDB5</accession>
    <name>A0A8S1VDB5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4485 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0610182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VDB5"/>
    <dbReference type="OrthoDB" id="78101at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055310">
        <property type="entry name" value="CEP112"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027831">
        <property type="entry name" value="DUF4485"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18871">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 112 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18871:SF2">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 112 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14846">
        <property type="entry name" value="DUF4485"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4485" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8174591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="216" mass="26071" checksum="25D5F5E3BA33B1D5" modified="2022-10-12" version="1">MDEEFIKVMLSIEETYQNLNKHQKIRVEAWTKKLCQVTTNEVWKKNRNLYARILLNQVQKGILSEPFDKRPPEGPLPKLNRYLVLSDQKENARPYTNKKDSLCFEQQQQQQQSVECPNLQEKMLKLLIDCRQYLEKKTDHEAILLKEQQDQFFIYLQEVNQPYILEQIDEKTEENEDQQKDTMQFLQYLDNFEKKTEKLRVETQKLLHGNNIQYNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VDH3</accession>
    <name>A0A8S1VDH3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0610202</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VDH3"/>
    <dbReference type="OrthoDB" id="298562at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="689"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="153"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8174631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="841" mass="100586" checksum="0360CF05A0587340" modified="2022-10-12" version="1">MQKIKQEDDEEFNFKRQFEIQQSLVEPKNLSKFKDILNQTIDLNKRKNKELASYNRNKISEESTNYTQQQLDFIQEQTDEVLTYQQYLKTKLDEILKAKLLTDRKINYYQDNLRHIKTKPDHVQKELDDQVKMMLKKTQNLTHQFQKEIDQFRSQSEQNQEKYSRDMENCEQEVKELQLKIKKCKQKNSDLLKQIRILTEQEEQKKHKLQECFKISEYIEFFMDAKLNVVDQIQGNQDQLQELGQTLQQQQMDKTLTRHEILNQVDEFYKTQDIAQLTNQIISTYHYLLEHQNMQTNEYNFFTEEKTLRQQQILQLNNELSQLKSICNIQLDDQTNFGSYNNTIIEGPQIIHENYQLNYHLHGYQLCLITIYQKLIDMIQRLCINVIWLSETTKSIPKSLDNKCMQYLKNFTKTDFSLYVPLATQSIPVNKSINSKHFGQYVCIGKRNGSITSEEIPSEQKIESMTRCPNEQLEKFKMHKDYEFIINQWNDLIRNDFITQTLQLDINFDNIDVTLSNHEIICQMYDQYSKLIKEADIQKHFQIQFQYYRDIMRHIYKNNQNFNLKTLTLKQKFNNEVTSPYLLQIKQIQLKRLQEQVNNSNLDDENNFKMHSNRTINASDDRKYHEYFIHQSSKIKKQFPALHDNLVTKTLQDIDIMKQKELNENYQQSDQISSDMDYLNQERRNLKGILNKQKQKPQTAHSQKSKTESHETSSTKTKIIKQVDLLHKIHKLSSQTRKLETSNSFNADLTKYTQNVKRKISDYFNSKLDMDFCHKEESRILQKPNQFEKTLSASRSVDPYSMTPYSEQIFSLDRIDSFRQIQDSTQLGSSKKDKAFLIKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VGF6</accession>
    <name>A0A8S1VGF6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Transmembrane protein 230</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PPENT_87.1.T0640147</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Involved in trafficking and recycling of synaptic vesicles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasmic vesicle</location>
            <location evidence="5">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Secretory vesicle</location>
            <location evidence="3">Synaptic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Golgi apparatus</location>
            <location evidence="6">trans-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Late endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Recycling endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TMEM134/TMEM230 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8176057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VGF6"/>
    <dbReference type="OrthoDB" id="307916at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044234">
        <property type="entry name" value="TMEM230"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008590">
        <property type="entry name" value="TMEM_230/134"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15664">
        <property type="entry name" value="C20ORF30 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15664:SF6">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05915">
        <property type="entry name" value="TMEM_230_134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0770">Synapse</keyword>
    <keyword evidence="9 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00007743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00024003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00024088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAD8176057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="122" mass="14181" checksum="6A1DFE3C6DE18736" modified="2022-10-12" version="1">MNLKPHKIGQNDEFELQNTSKEDSHITISSLKEQQLFHLNAIPKKIIAITIMLTILGITFLIIGLLLLIKQPSNYDQYLPLLFIGILMTIPGIYYSYKIIQFAYSRSRQEQEQIIQELPIDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VHJ3</accession>
    <name>A0A8S1VHJ3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">RNA-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0640120</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8176003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VHJ3"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13948:SF3">
        <property type="entry name" value="FI21118P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13948">
        <property type="entry name" value="RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00641">
        <property type="entry name" value="Zn_ribbon_RanBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="10">
        <location>
            <begin position="213"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="266"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="378"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="511"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="59"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="75"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="512"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="531"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8176003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="645" mass="75220" checksum="96C11735D5446051" modified="2022-10-12" version="1">MDFHHKNNERNNKYGNEKSNYDRNEKHNDRNNDRNNDRNNDRNNERNNERNDRYDNKNDRKRKDRHQKRKNRKYSSSSLSRPSSSVSVSVSQSESSSSSEKVPRYRPKNHDNMVLLLENLPEDLTKQQLDSAFNEAAMEANVIPHEEISIINAFGQAYIKYCTVDYARKVLIYLKGKIQIGEHTLNVDFYDDTSGRQRRNRHELKNSLINQPPTSYDWICDKCGYENFAKRHKCNKCLNPRNINCKILSVMMAPPTGFTDDSICNTSLMIKAKSIADATDSDILDIFAPLAAVKDIRLVKNKVPKDPRMKEQKDFAFVEFFSVEDAENVYRYVTQNEVRLHGDQLIIQYSRNNRASRYEDHSKPFGFYPNNQFVMQPQGPLQPQIPSQIQSQLNPQQQPQQSQDIIQQQQQTQMQIPMPIQMQMPIPPPMPIHQIPQIPSIQHNQPQQTQPIPQLPIQMPEVSIVQPIVQAPYQAAQLAQERIILKQEQKLTLSTPPMNLMSQLPQKIFNPKPQQQQQPIQQQSQQQKKQQKVEETEEEVKSDKEDEQPSPPLPTKTINKVQPQLTEAQIRKKAELELKKWEKQQQTKKPEVKVPPSKPIINQVEAQTRLILYICPICRRKFPSEEVMNYHSLNSEMHKQKLLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VI49</accession>
    <name>A0A8S1VI49_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">cathepsin L</fullName>
            <ecNumber evidence="10">3.4.22.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPENT_87.1.T0640226</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="11">May be involved in extracellular digestion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Specificity close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity.</text>
            <dbReference type="EC" id="3.4.22.15"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.15" evidence="10"/>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8176213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VI49"/>
    <dbReference type="OrthoDB" id="387093at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000104">
        <property type="entry name" value="Cathepsin L 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035762957" description="cathepsin L" evidence="12">
        <location>
            <begin position="18"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="14">
        <location>
            <begin position="29"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="13">
        <location>
            <begin position="112"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8176213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="309" mass="35719" checksum="119DCD358CF7F5EE" modified="2022-10-12" version="1">MKQYLAIALVTLLMTVGYYNVSEENKSEFELWTTQNNKLYTESEKMYRMGIYYSNKRMIEEHNLRDDVTYKMGENQFMALTHEEFVDLYLQKNLNIPMDKIQVNIPEVQLEGLTAVDWRNYSSVKDQGTCNSGYAFSVSNSIEAWYGIMKYQKIYPSTQQIVDCDFNSSGCYGGYNGYAMEYAFRVGLSTNQTYPYVDREQNCKQTRNGTYFISGYSFVGGSQQQLQQSLSKYPVSVGINADNWQFYSSGVFYNCSSNATTHYALAVGFDNESNWIVQNSWGSGWGEKGHIRLYQQNTCGILNYPWQIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VIM4</accession>
    <name>A0A8S1VIM4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mei2-like C-terminal RNA recognition motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0630147</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VIM4"/>
    <dbReference type="OrthoDB" id="417481at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12277">
        <property type="entry name" value="RRM3_MEI2_EAR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007201">
        <property type="entry name" value="Mei2-like_Rrm_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04059">
        <property type="entry name" value="RRM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mei2-like C-terminal RNA recognition motif" evidence="1">
        <location>
            <begin position="93"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8175619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="208" mass="24753" checksum="130B7FC6C5AADEA5" modified="2022-10-12" version="1">MKRKLYKLSLFKEIDEFNQKDDDGNYSDDSSISDEFMNSTLEAYQSQIFISSNQTFSSIHSEQLQFIQGVFDKSEESEQSFDFNITNIDSDNRTTLMMRNIPQTYTKEMILMEIDPKFKNKFDYFNFPFDGTTNPGYAFINLKSKSYLRDFYTYFNGRKWKNIPSNKPCYLKYAKIQHKKFKQINPQIYLQQSSVIKLVQNQKLQCNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VJJ3</accession>
    <name>A0A8S1VJJ3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF676 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0640088</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8175939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VJJ3"/>
    <dbReference type="OrthoDB" id="273452at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000343">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022122">
        <property type="entry name" value="DUF3657"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044294">
        <property type="entry name" value="Lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482:SF5">
        <property type="entry name" value="DUF676 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482">
        <property type="entry name" value="LIPASE ROG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12394">
        <property type="entry name" value="DUF3657"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF676" evidence="1">
        <location>
            <begin position="498"/>
            <end position="680"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8175939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="759" mass="89617" checksum="1A555D2B57485E45" modified="2022-10-12" version="1">MSLRSVVEIVTHVESFRNVDLYYQGVYFLRITIHNDAPQDINKIYAHPHDPCESYKTYPQEAQQNVNPPKYYETHIFRPASIVTSNSAFYTKAFFIKFCEEEIELNDICNFRIEFDAGPKKEQSLIMQVDLMFFDCLNSQKDQPKQEPLYTKQDKEDYAIPDGKIQATAKFKIKNVLQPNHQFVPIIFEEQNFCQANMVVHTITLDYRFRTHPIQLFQFARLKFEERNQLLEGKVPNTLDKNKNQSKPLINSDQISMYETIQKQYKDMKGLDYYSMQQEFVESLKGYYEQLYQHYNLIYSKCILEKQRKHFKKYLYPPLKLIVPEYITSETYQNIKGQLTRKELNQRIEEKFHTADPETIIHSILNETNLISCQLFQMWIKVLDLYRISPRFCVALLQFDYQKIIKNRWQQFCIKSQTQQLLDKNLGIEHKSKSEKMRQETKVQELGVEDLNSYPKIETQPIIFEEFTPREDKDKNEENIVDADITNSEYDIMSYRGIHLIVLVHGFQGNSYDMKLFKNYISLSHPEAMFLCSSINEENTEGNIQEMGEKLATEVINFISENCPENTLGRLSFIGHSLGGVIIRASLPYLDKYQDKMYTYISLSSPQLGYYYNASKIVDAGMWVLKQWRKSKCLEQLQMTDNRNIEETCLQKLAQAKGLAWFKNVCFFSCIQDSYAPYDSARIQLSKEALEDQRNKPYVQMVKALLRHLENTNVYRIDVNFEIQEKNLDTLIGRTAHIQFLECQPLLRMIVSLYDQFFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VKK9</accession>
    <name>A0A8S1VKK9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="7">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0690227</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="7"/>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VKK9"/>
    <dbReference type="OrthoDB" id="292964at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02674">
        <property type="entry name" value="Peptidase_C19R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF24">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="68"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="442"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8178498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="503" mass="58987" checksum="6113DE064A769D8C" modified="2022-10-12" version="1">MGICQTKPKVNEPQTKMKKMNTEQRLMPKPSRILDVNYKQIPNKFRRQATQRRVQQINQFDTDTQGLFGLTNLGNTCFLNSSIQALSNTQPLTDYFMSQLHIKEINPENPLSSQGNIVKSFAQLIRLLWQKVDQYQYDDMKVLKPTQFISTIGNYNPIFAEGTQEDAHEFIAFLLDMIHEDLNRIKKKPYVEQAKFIKFPTNEDAEQEWKKYLSRNQSIIVDLFQGQVLDTLSCLTCKSSRYCFEPFMYLSVPVLNRECDLLECIEEYLKKETLKGEEGWNCTNCKSRRDSNKKIDLWTMPNILIIHLKRFKFNSQFRAKIRSLVKYPIQNLSFENLVCTKQIEKPTYDLYGIINHSGTLTSGHYTAYAKNRDDLHWYHYNDSLVTPVQQPENLSDAYLLFYFKNSVEEFKRQTLEGINVSQKRKTIKNTIVIKQTILNMQSQQSNSNISDNKRNNNNNIAPSISSQAQKKMLFRKQTKHFQIVKAQEQPSFQQESYVIQEEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VPV1</accession>
    <name>A0A8S1VPV1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0650187</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000065">
        <property type="protein sequence ID" value="CAD8176646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VPV1"/>
    <dbReference type="OrthoDB" id="27140at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000061">
        <property type="entry name" value="GTPase-activating protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000031">
        <property type="entry name" value="TBC1 domain family member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF337">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="41"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="476"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="476"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8176646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="636" mass="74237" checksum="734750F9C66D06C5" modified="2022-10-12" version="1">MEAITQLKKFIGHHFNVKSFHKYMINNDISDIETLIKFCQLDNTGQRFNLWRLFFKIYSLEDTVYEKIGKLTKTRDTYKQLEQKYSRSQDVISTTKIEEQSKKGPLGGPLGGPLGGGSKIKTATQNPMEDAELRNEIRKDVERTYQEIQFFANKKILQILTSVLFIWSKENSEISYRQGMNEVAASLIHVYFQEALYSDEIDKIQGATEEDKQILLEFNSWEYAEADIYTLFQKLMNDAQHMEMFRPNYTEAQKIKLQSKKPSAILTRVSKIQDILLKQVEMPLFRHLKLLQVEFQIFLLKWIRCMFTRELHLIESFKAWDAIFYDFYEQKTETLFFVDCIAIAMILYVKQPIMEMEDSSQCYQRFLKYPPVSNLPALLESAINIRAILLNQKSATNLDDQNESNQPSIYSDKQFNPVFFAQITDKVDGVDAKVVKITSEKPQFRTKGDVDLVYKDETSPDKSNQSQQQFINPLQPKQISQQQKPQQQQQQQQQQQQQQPTSQTIQSSNSSSITTNFFDSSLSSTNTKTIKTQFQVEEQTQQVKKDKASIPMKNNIQIPIQQAEEMFNTLEDALKFMKQFKDHSNYQDLIRNLTEMKDIFETSLNENSLTQRQQQQNQQDDNGISGKVAGFLKKKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VU11</accession>
    <name>A0A8S1VU11_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0750103</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="9">Microtubule inner protein component of sperm flagellar doublet microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RIB43A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000075">
        <property type="protein sequence ID" value="CAD8180910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VU11"/>
    <dbReference type="OrthoDB" id="308880at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008805">
        <property type="entry name" value="RIB43A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14517:SF6">
        <property type="entry name" value="RE41410P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14517">
        <property type="entry name" value="RIB43A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05914">
        <property type="entry name" value="RIB43A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="105"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="107"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8180910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="143" mass="17441" checksum="2D8953F600706321" modified="2022-10-12" version="1">MKRTKETIFRQHPDWQQRQGISGLKEFDGEDLQYDARNKYNKEQQKQWIQEQIEEKKRKQEQEKAEEMAYAQQTLEINRFRGMLQDNFAARKTDIGVATKQTNLQFAKEKKDKEERDKQERLQYEKNERDLLLERGRKQPYNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VU41</accession>
    <name>A0A8S1VU41_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0720202</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000072">
        <property type="protein sequence ID" value="CAD8179753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VU41"/>
    <dbReference type="OrthoDB" id="308675at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="297"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="317"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8179753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="329" mass="39006" checksum="39D5BEA641C9330F" modified="2022-10-12" version="1">MKFHQLRLKDVSYSNTQLKQEDRQIKTEFGNLSTSRAKGAVIKQFVTEDNDTGRSCNTEKELFTKMQNLEFQNGLFQMQMQHIHKRKNIEDFTIRQQLTQIQQDLQCIQNRVQSVKNKSSRKKISLSSSSSSSEDVGRLQIGFNYRNNQDQMLSNYKELYEVCNQHEKLNALFSNYCGSIKTIEIDIVQQNFQLLIQQLIKMMIDILKKFNSIKIDQLKNTPDKQQCNFEFGTFNKEQKKTLRTTESPSFKRNQDLEEMKLKLNDHYDKYEQEKENKGQDINKKQDKDLQNQKKQIVHNNNNNNNNNKQRIDLIPSLKRKAKLRQQHNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VX12</accession>
    <name>A0A8S1VX12_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0750014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000075">
        <property type="protein sequence ID" value="CAD8180755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VX12"/>
    <dbReference type="OrthoDB" id="339469at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8180755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="93" mass="10900" checksum="170870C385301BD1" modified="2022-10-12" version="1">MISNKYKQPNQQMEYSKFIEITFSARYRLISNGNVAQFILSCLWNYYQSPLYGNDETSLQNQLYFLIMPLTILLPLTLIQNMFVFTKVSAIAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VYY0</accession>
    <name>A0A8S1VYY0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">H-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0770113</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000077">
        <property type="protein sequence ID" value="CAD8181867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VYY0"/>
    <dbReference type="OrthoDB" id="308347at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019019">
        <property type="entry name" value="H-type_lectin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09458">
        <property type="entry name" value="H_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035834772" description="H-type lectin domain-containing protein" evidence="3">
        <location>
            <begin position="21"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="domain" description="H-type lectin" evidence="4">
        <location>
            <begin position="37"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="552"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="271"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="602"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="571"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8181867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="734" mass="85751" checksum="C03110FAF18CE3EB" modified="2022-10-12" version="1">MKKEFLIVMTLFMCINGIQQSNGILDLQNLKAHKLFTKNVQFDQEFDEVPKVVVSIKQIIGEQYELYTRTSNINLKGFDLQIISSSDIDDIKINYLAINDNSLNIVCENYQTKKDQIIVPFNLEHSQVAAFITGLKLQKNENVYFKLNSITNSQIVFETKQKAMGLCLVIGDQDKIAITKQQITTTQKDNKLEFNVENIEDSIKLPLENQILSFDDIVNKLSVETKEEQQQTQIQNEEEQYLDIDSIQIETIGMNIIQQNIEDDVIQSVEIEYLQTQKQIQEDQLLKIEEKQIERVQEKIAETVNEQMQLEQLEEKKFLAQMNQVNIENVQNIEDSIQQEIAIQEIYQIVPQTVDELDMDALYKEFTLNQELNDDLVAYNKNIISQQQQQQQQQQQQQQDTIQVIESVDIVQESLVNQIQSQVIIDTKQVETQQQIPEQKQLQIDVPLFDEDLTFDNIDIELDQQKVEKQQSASPAQHIIDIYNAFANEETEKLNQKENQEHPFVEVETETQPEEMPDELDDQLAGLRDGLEGIDFSLFFDEFSKPNELVANQEPEQKSEQELIQNKKSQQKYEEPKEFKQPKEKIEDVMKDLQINNDIKTTEDEKQLITQLKQKSAELKQKLQDQYETVPKLEQAKKQTEVSIHPIDEQYPKIESDSKIDYEVESKYMEWSMSQDQKIKKIGLDLTEDDLKNPRFSLLQMDQKSELDIQKQINDFLGLDDLSFVMIKQNLRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W116</accession>
    <name>A0A8S1W116_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0770176</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000077">
        <property type="protein sequence ID" value="CAD8181962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W116"/>
    <dbReference type="OrthoDB" id="306476at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="75"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8181962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="185" mass="21640" checksum="1F69890CA6CF9B78" modified="2022-10-12" version="1">MITVQIRTPKVKLSKHRIQNSQPTILNYSNLNSFHDFFNNSQHSNEQRQRIFNSRCKTENTQMEVSIKPNKQKLSSQSTTISLASSTSPNQGMKNEKGNSNSSFKIQQQQNSVNSFQENGTNQKLKQQLQKIVERTNKMMNNYQKVSKKWEQREFELKQEISTLKKIIQKQNVQLQKHNLNPICL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W7E9</accession>
    <name>A0A8S1W7E9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0840216</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8185190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W7E9"/>
    <dbReference type="OrthoDB" id="435695at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000008">
        <property type="entry name" value="Putative TBC1 domain family member 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000147">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF9">
        <property type="entry name" value="GTPASE ACTIVATING PROTEIN AND CENTROSOME-ASSOCIATED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="1">
        <location>
            <begin position="83"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8185190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="344" mass="40537" checksum="33793859E8E327CF" modified="2022-10-12" version="1">MNQNKENEYLVDVQSYFTNKCITSARSTSLHEDNTMKFSRNKNLFEQKQQENEKELKKKWSDIAKDWQRNKRDVKVFQLAEFGIPISARKQIWPLIVGNKLQITKELYNILQIQSKTFKMDSRKPLGRLSSFNHIKQDLGRTFKNESLQKIFHPGGPLSENLQTILEVFCLYRPDIGYVQGMTYVGSILLTYFDDYQAFVIFSSLITHPQLISIFMLDEQNLQIIFQQFEKLIKLNIPQVYKLFKQYNINCTNYLLDWILTLYAKPLNPDIVGRIWDRMLFNGTHILWKCGIAILKILAPKFKDQEKTLQSLKNPDIKDDDLLQEMKSVNYPFEIQEVLGNIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W842</accession>
    <name>A0A8S1W842_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0840166</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8185107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W842"/>
    <dbReference type="OrthoDB" id="285315at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8185107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="100" mass="11765" checksum="F2E52951782839DC" modified="2022-10-12" version="1">MIKITSPLLNHRFKMVRVQCKSYDNVELIRDQIMDVMNEYLQENDGKLINVKVISNNQIAVYFVTGIEMDDTNWEQQISRGPLQKFTILSNSIEIRPSRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WA10</accession>
    <name>A0A8S1WA10_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0880126</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8187058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WA10"/>
    <dbReference type="OrthoDB" id="62528at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051493">
        <property type="term" value="P:regulation of cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010441">
        <property type="entry name" value="CH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052111">
        <property type="entry name" value="Spermatogenesis_Ciliary_MAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12509">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12509:SF8">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06294">
        <property type="entry name" value="CH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="2">
        <location>
            <begin position="15"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="817"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="832"/>
            <end position="844"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8187058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="844" mass="98580" checksum="EE1D80701460CA6A" modified="2022-10-12" version="1">MSSNTNTQTFQPVGKALPREIIKWIQGLDLSYSVKDPRRDLSNGFLIAEIFSRYYPGRVQMHSFDNSQKDERRQNNYQQLELFFKKNDIVIPNHKGFVEILDNDWNALYNFLVSIYSFLTQRKVINPPLASYVNSKEFATSTQNTQTFLLKEKGIEKLQEEQKQEDQFQKEQLSEKHAPLSVSSKRTNFMRVPSKPISQNLENLNYQIEVKNISYRPVQGSLLKLKEEQQQQEKQQEKLNSNNDRQIQDSFQKTPMDENKREATSTREKQLDKSIYDILNEALTQRQQFPQFVQTVQFKQNATALQSFIDLIDIYPDDFVNGFFQELITQKEAYINFIFKDTNEVWKFFKFTFQCIQNLPVQRVHVCIDLVNQFGARSLQKDALKTRSLFLEFFLPEICILIKQSVFFEKKQLLIQMICSFQEPMFKYQVIQTMKQNLSVEHFMQCLAVFSGFEQEQSELWNDMKRYGYIYFLSASTSLSSSALAILCNAAKANSQVKIDKHVMALAKNRWWQNRCLCVILFSEMIRGVIKTPNYQNLIKSPQQGQKFFSIENDRIISDLKAQVNHFSKGIEIASLPIDSDILMTQSLIHIVDLLGDSKILLELFVKIVLEISQESRQWALFSQDQQDEYDFFIPSDKSFKFKLNINSQYVKQVSFSILLHLVELLNTQKLLNLPLGHFELLHWCFQNTDFKIQNMESCEQIAKVTKEYVYLGLSDPTILLYANDLAKQLLDLQFKAEVQIENHDKIFANSIRLALKAENDQVLNNIVEFIENLVAESGQSASDGLKNFIRSVYDELEQMNPSPLSNPKISKCFERVNDRPYQSPHPDVSDVQIQDEQQSDFQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WL25</accession>
    <name>A0A8S1WL25_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0940003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WL25"/>
    <dbReference type="OrthoDB" id="323880at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8189397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="114" mass="13763" checksum="81F548CECD8EF3AB" modified="2022-10-12" version="1">MHVYEMNSVNQQFTKTKDIIVNGCKINQGGLFHNSLLFIKSKQLLVNKHGNCVNLIRKTQNEEFKVEQSQVFILISTKMWRQFLQKVHLLFLQYPPLWFFLIKYFYDLYFLPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WNV6</accession>
    <name>A0A8S1WNV6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0950036</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000095">
        <property type="protein sequence ID" value="CAD8189909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WNV6"/>
    <dbReference type="OrthoDB" id="293593at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8189909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="551" mass="64660" checksum="574602BFF0D1AAF9" modified="2022-10-12" version="1">MKKNIISNEMRLKSAVQFKFQEFMNLKPAPSITTRLQTGTTTSRNVEDFAKPLFSEPKFLGVLRRVSSQVSLDKPIEDVYQSQNQSLKAVSSERTASQKIIVTSENMQTQEIKRRMKFDPQIRQQVSILKIKILKNVPLKKFLLYLVGKEKILFELLRLKQMPQAIDIMQSIIEMALGTKNIIIFIEILLFIAETLENSNQIDLAIHFYNQVRIGSTYGKQTLDIYKMRSLVGLANCCMEFQCYDCGVKFLKKCLQYAWINNNQEFENIVYQKLGMLYFYLGNIEKATFYHERSINYDYELENSPLRQLSCDTLKNYLNKNFPKNCAENINNLFLSKLSLKFHFDIAQLSEDLIKGSELTPSPRIFLSSQDCSRRMSISTEFRELANLKINGQKLIQQILTQQEFDFEVYTPKHSFRNESSCFDFLKNKKIHPQDVFHDQKYKNNPFVLNDLGVEPNKHQLNFAEYLSKYKLSLQKKVDIRLQQKFDINIEQKLQYAVSSNYKTFERKNKILLTHKNQENSKKNKQLDETKKFNSISTLYQKLINKLLTVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WTE3</accession>
    <name>A0A8S1WTE3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1000146</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000100">
        <property type="protein sequence ID" value="CAD8192041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WTE3"/>
    <dbReference type="OrthoDB" id="4833301at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="164"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="517"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8192041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="672" mass="80154" checksum="10E349A7E5962453" modified="2022-10-12" version="1">MLNKNGTYTKSKFSSTMIVQSVDNRVRYSQGPEELGKRTKLPLDITLFVMAVEVERLQRENAQLKSDLQSNSDSNLDRVQYESQIRDLMEKIILQNNSQSNLLIDIEKLQRKVQDLEDQLKRQQQSQKEFDPNLRAKLTQAELQLAAFQKQFNQQVNLFGGQSADQVMRELEELRRKKQQFEDLSRQMNGRNVNELLRELDDLKKKQTSFEDNSLLRQQLLQYQNRIRQLEQKLSEMDQYINTIESDLNRFESENKRLTISMSSKIQSENNETTLLRNEITKLNNDINKLWSDNETLVLEIDSLQMKLRNLNDDELRRLRNEITRLQEQNNHLESQIMSLQSQNQSLISEISQLNMTIEQYQYTINDVNKQKYIFEEYKEKIRQFELKFQELQLQNERLRQQDHKVAVLTTEIERLNNLIKQLNTDADEWKQKYQRIELALMDYRQIEKTNRDAVTKNELLLDEIERLKKILEQKQYDMDQLIKQYDVIQSQISTYQTNVNASVNRIQEYELTQRETSTILQDNERLKRQLQQYQQQISTLQIQVQEQTTKNQQLIAQQQQSYMINNNNTEIDKLKRQIHEYENKIAMLSLELTRVRNTKQNSNSYDKSDKVMELLTTIVMMSAELDNLRGITYEKSTVQSQRLQDNISKSIRSQSINNQQANYSYSNWKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WUJ4</accession>
    <name>A0A8S1WUJ4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1010162</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000101">
        <property type="protein sequence ID" value="CAD8192447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WUJ4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8192447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="161" mass="18849" checksum="DB721855E04E9CA6" modified="2022-10-12" version="1">MVYLINNYELCDDGNNGNNVGDDRFSSMCLIEDNQNCNNQFFQSICFPKTQILLTYLNHTNSNQYAELSFSNQVNTIESNVNSIDCIHIKLSTKNYSVTIKEVVGINQQVYLFLFMKFKLNFLIQQLIKYYQLISIQNQRILIILKLPTIKNISYFNLLQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WUT0</accession>
    <name>A0A8S1WUT0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1050081</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000105">
        <property type="protein sequence ID" value="CAD8193864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WUT0"/>
    <dbReference type="OrthoDB" id="270720at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 5-kinase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8193864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="377" mass="43503" checksum="57654418E9DBCA26" modified="2022-10-12" version="1">MQLNNQMDAIFQQEKEQILKEFESKLHRLQLQVQNKQYQDGSEYFGELNNDLRWGQGVYYYFTGDVYAGQWANDLFDGDGTYIFASGERYQGQLKEGKKHGFGTYYYLNGNSYTGNWANDKKQGRGIYVYAQTNEKYDGEWNNGLRQGYGTFTYQCGVYDGEFNNNVKEGKGVLKYKSGAILDGEWKKDKVEGEAKLTLPNGDYYKGTWIGSHMNGYGRYQYQSGGYYEGWWKQDLFHGEGMLTYENGDIYRGEFQNGLKHGKGIYLHQNGDQYEGEFKNDMREGTGVLRMKNGDIYEGEWSNSLKEGKGIYYYSNGDRFEGNFLGGKRSGHGILYWSEKQFVNGFWVDDIMNGQGVLMKENGDKLQALFKNNKLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WZI8</accession>
    <name>A0A8S1WZI8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="27">Dynein heavy chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">PPENT_87.1.T1070164</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000107">
        <property type="protein sequence ID" value="CAD8194792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5593012at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.710:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.30:FF:000002">
        <property type="entry name" value="Dynein axonemal heavy chain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1220:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.180.20:FF:000003">
        <property type="entry name" value="Dynein heavy chain 12, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.100:FF:000001">
        <property type="entry name" value="dynein heavy chain 17, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1120:FF:000001">
        <property type="entry name" value="dynein heavy chain 2, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.20:FF:000001">
        <property type="entry name" value="dynein heavy chain 2, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000063">
        <property type="entry name" value="dynein heavy chain 6, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.720:FF:000001">
        <property type="entry name" value="dynein heavy chain 7, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.280:FF:000001">
        <property type="entry name" value="dynein heavy chain 7, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.490.20:FF:000001">
        <property type="entry name" value="dynein heavy chain 7, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000049">
        <property type="entry name" value="Dynein, axonemal, heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000362">
        <property type="entry name" value="Dynein, axonemal, heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041589">
        <property type="entry name" value="DNAH3_AAA_lid_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46961:SF8">
        <property type="entry name" value="DYNEIN AXONEMAL HEAVY CHAIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46961">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 1, AXONEMAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17857">
        <property type="entry name" value="AAA_lid_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0243">Dynein</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Dynein heavy chain linker" evidence="16">
        <location>
            <begin position="996"/>
            <end position="1408"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain hydrolytic ATP-binding dynein motor region" evidence="17">
        <location>
            <begin position="1541"/>
            <end position="1866"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA 5 extension" evidence="21">
        <location>
            <begin position="2031"/>
            <end position="2175"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain 3 AAA+ lid" evidence="22">
        <location>
            <begin position="2400"/>
            <end position="2491"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA module D4" evidence="19">
        <location>
            <begin position="2556"/>
            <end position="2806"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain coiled coil stalk" evidence="18">
        <location>
            <begin position="2820"/>
            <end position="3170"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain ATP-binding dynein motor region" evidence="20">
        <location>
            <begin position="3199"/>
            <end position="3421"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain region D6 P-loop" evidence="15">
        <location>
            <begin position="3665"/>
            <end position="3782"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA lid" evidence="23">
        <location>
            <begin position="3814"/>
            <end position="3953"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain C-terminal" evidence="24">
        <location>
            <begin position="3961"/>
            <end position="4267"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="922"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="2835"/>
            <end position="2883"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="3047"/>
            <end position="3116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF12774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF12777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF12780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF12781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF17852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF17857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF18198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF18199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="CAD8194792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="27">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="4271" mass="495813" checksum="3941A652B832BEB0" modified="2022-10-12" version="1">[encoded data, 4271 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X0W7</accession>
    <name>A0A8S1X0W7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1000064</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000100">
        <property type="protein sequence ID" value="CAD8191886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X0W7"/>
    <dbReference type="OrthoDB" id="295027at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8191886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="82" mass="9348" checksum="4FACFB8104D2D6E7" modified="2022-10-12" version="1">MEKKQESGQNTLMSLWSKRTSIDTDLNETPSVYLDMEYSQDIQKYNFVIGILKPGQKRIGLIAINKNKLSIAGILGIKKFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X6I8</accession>
    <name>A0A8S1X6I8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1060113</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X6I8"/>
    <dbReference type="OrthoDB" id="1711136at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047126">
        <property type="entry name" value="RNF141-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109">
        <property type="entry name" value="RING FINGER PROTEIN 141-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109:SF5">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="196"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="225"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="267"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="4">
        <location>
            <begin position="500"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="471"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="479"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8194276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="565" mass="66351" checksum="61CC5F548C3588FD" modified="2022-10-12" version="1">MIQMPESQLGSVRMRPSQIQIEDDQQVSTRRRNFLSPTFSQNLPLFRELQSRQVQNREQDYSNSIITLTDDKVISCHAQMIKSLMFNLFLQAFFVNYFTYMHWKYSHRYVLLQLWIQDLITIFILIYYNYRKGDDCIWQLIETMFLFFFKIFIVFYYEVQEFKIYFITIMMLAVSSLLLIMKLVQKIKAPTKTSTALILQFLKSMCCLQLLLITLKWESKISWSWIQIFLLLWVFLVVCALLLFISFVSCIETLVNIIKKKQQCHYLAGNLWIFIMLIGFTLFPFLFILRTAEFYEGNQLISLTETAKYIVVITLFSLFLLIFTLCFYRQIKAYLKDVQGISDQNDIDQQQTVQSPNSRQLNSPHQTKKLEFVKLGIPLYLIKLSCTYFAVLDKASFEFKKRQKSHQDLESGRSNLQGSTMRETWKKILQKPAASNSMIINKKKDELDLDIQKPAITDNVKVKREEIPVTVSKQEQEEEPVRSIKSIKSDGESSENQEKCLVCYENTPNIVFVPCRHGGICQQCAEDVIQKSNECYLCRKTINQILKVQKNVNKQLEVKEASMLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X846</accession>
    <name>A0A8S1X846_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein 135 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1100138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000110">
        <property type="protein sequence ID" value="CAD8195786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X846"/>
    <dbReference type="OrthoDB" id="291792at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026749">
        <property type="entry name" value="Tmem135"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12459:SF15">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12459">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 135-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8195786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="204" mass="23667" checksum="8AC473FA1A4096C1" modified="2022-10-12" version="1">MSINNRGLSKEFQERIINDLGIVECNDFHDGTCLYASFVRLLQLIPKSYKYYIPIHLIPFLIFKRKRVIQRPLKTISVAFYNYAKSVLFVSLYVAICKYALCKLKNIRQKVDGLNPALAASVACSALFLESEGRRQEIALFIFPKSLETAWRLLKKRGYVSNIKGWELFLFGLAMGIINYFYHYDDAAIKSTYLTIFNNFWGKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X9L3</accession>
    <name>A0A8S1X9L3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1160099</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000116">
        <property type="protein sequence ID" value="CAD8197760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X9L3"/>
    <dbReference type="OrthoDB" id="298853at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="9"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="300"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="303"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8197760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="391" mass="45259" checksum="2E7B18A18FE6F961" modified="2022-10-12" version="1">MSEEDEVICEQCQTVPTQFVILDCEHKFCLYCISQAIHKDDSGVPCLKCNTPTVLDHESLQAVQLTKESQSQASKTQPNYNVKPFTTEKRQYVQPIQEDLTIKGHPCLEKLNLAVERSLDKIRNIQFDKQQVKDKCQTQKNDIDTEFKALHMYLDSKQEEFHNQLSNIENTYLNELTKDEEVQQLDIEEMSIIKNEIKAILKSQDSQDIYTFKQLVATVDGIVQDKQLSIKQLNLFKQMQDELQQLSKRQAQFYQDFRRFFNILQPLQVSLVQNYASPIKVEYGGSITSDIQEKIRELKNRNSRSMNTSQLNNSRSQNSPPKNTFLNEEINISKKLSREQVIIMQQRKLGTSPMDAKTVLQSSMKHNNKSNSKFNKILNSPLTLAGFSYLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XJ91</accession>
    <name>A0A8S1XJ91_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1260060</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000126">
        <property type="protein sequence ID" value="CAD8200927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XJ91"/>
    <dbReference type="OrthoDB" id="3531824at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="15"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="392"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="308"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="367"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="392"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="407"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8200927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="530" mass="62866" checksum="EF3367906FE9A79B" modified="2022-10-12" version="1">MEYSQLKQTQFGPYEIKQDKIGSGNAVTVFKALNLDWNTDCCVKIVDKSDQSQMKQYQIEKQISSELKWVFHKNLVRIYDIIEKDNYIYIFQELCDQDLGRYKKQLDREQILDLIIQVASGYIQLMERNIMHRDIKQDNILIKKDKNGQIIYKLADFGQSKKCDEESNRITTRNQQGTQSKKAPEINESDYSYLADIFSFGLVLLELYLNIQIDKIQHKNCLLNYLKIKSFIQIKEMNDENKQQFEKIKNIFEYNKDDEIIKIIDQLLKYNKEERICWQNLLKKAQDIKKIMNDTQGNIQKKITLQDQQPHQLQQNQLQPYQSQQHQNQPHQLQQNQLQPYQSQQHQNQPHQLQQNQLQPYQSQQHPKVSNFQTRQTPKNQQQYLCQIKGNGIQNQPQSNLPVQYHQPQSNLPLQNQPQSNLPVQYLPQSNLPQQIHQPQNHQLQNNQQVGNNFCQTKGQITSFQNKHIKIQQNQQIIPLQNQQPKILYKSSTQQQFCNQGTINRQPSNNMAFIYSPQNQQIQKIIYRAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XJG3</accession>
    <name>A0A8S1XJG3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1280091</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000128">
        <property type="protein sequence ID" value="CAD8201590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XJG3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8201590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="481" mass="57621" checksum="59D2E05D09A4389E" modified="2022-10-12" version="1">MTQRFEQIQFNSDNFIINYHIIDEGQEINQFYIYFSCKIDQTIYFKKFSEENTIYDAKCIFSQSLQKQKTSISIFSQDTQLKDDQLIIHFKQQELFIYETKLISFHTRNGQVLKQKVYKACMKVKEMKNDFQREISQQSCKYEFNQSPIDDEDKLLSDITDQTEIVLDVDFQHNLKLINNELKTQQFNVQFYQTEIVSSIFSKFLKGNLQYYYNGQLIDINKSFEDNLIQNNSCVYYGKIYNFTFKFTNNNQIIKIQVKSLNTKIEDAVQPILKENHPQNFQFSCNNRLINLEKTFSQEKIKEGVIQIELKSLIVNIYDKNKIYKYHVLPNLTIEKLNKLINNNQNKWLFKLDNDDILQDNIELSTLKNSNGEVELIYKEYIKQNTILHQYQDDVSIDFFVTQNIKTIENSSLVIPIEWNDQKFFETVKLDQKVEEFLEDFKKQKKLSKIFFFQDGEIIDINKKFEDLRDIEYSFFEIISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XKT3</accession>
    <name>A0A8S1XKT3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1270096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000127">
        <property type="protein sequence ID" value="CAD8201348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XKT3"/>
    <dbReference type="OrthoDB" id="320200at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8201348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="497" mass="58620" checksum="0ECEEB8501F3B0B9" modified="2022-10-12" version="1">MNKITLKFIDKAIEQKYQLEHQQPKRKTHLKILLIFFIMLQIIKLIIAIIINNYKAVYPTLGMMGFTTLSKFFNIDKEYHQRALIIYINICFSIYVIFFDPQSDPPTMYFRGANQMVINIINIIGIEFLDSTITIILLYSLRIFHLILNTSSIDITSIIFGFGANISLIVMVYFYHKAIRSQFLLSKIDQRWENILKQIIHNQQFILINYQVEKLQFQSVSSTFSKTIQSQEEVIKFLREAKVDNGSLEQYIFHKLQDFSQQYIEIINHSINVKFDKQLLQVYFSIFFGNQPTILIQLKQSQLQLKNKDVQKVCQLYLKLLTVFIKIIKENIPFDYNQFHNLANKIQFQNKITSIWNQQMISKTISLKKSILKIQKFCNPNTKIQLISSDLIIYTIPKIFHLLLACIVDSSISELIIIKYETVETQLKLIKIQGKFNIRKLNSYTIKIKNCLLLICKEINVQQFCINIELNDEILQPFTNIIMPQLHFNYSKKKLTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XKY3</accession>
    <name>A0A8S1XKY3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1280073</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000128">
        <property type="protein sequence ID" value="CAD8201554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XKY3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8201554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="94" mass="11734" checksum="52C67162EAD8D0FB" modified="2022-10-12" version="1">MKIHKFSKKNQKLNSWAIGHNFEPPDNYLLSNFRQDNQLMINFQYGIHESNKFQKVIGWRMKKTAQQQRHKQYKRIYLYYNRDQFKIFEMMKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XSK0</accession>
    <name>A0A8S1XSK0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1350021</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000135">
        <property type="protein sequence ID" value="CAD8203768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XSK0"/>
    <dbReference type="OrthoDB" id="282957at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8203768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="177" mass="21242" checksum="4740532129E1EEDA" modified="2022-10-12" version="1">MLKIFRQANLFKPQFMRRYDQANSPLHIAIGDTSELRTKLFPEKRRIPGRKGKIILAIGVLFQVWGIMHIVEVRRQFKRKELELKKMQRKSLPFYQAMQDIRYLAAEDKRNILIEELFAEHGSDYIKQITDIYFQKDVWVPFKKRAAHQYTRATKDPYPYFDIPGSRFLHGYDVYNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XW53</accession>
    <name>A0A8S1XW53_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1410106</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000141">
        <property type="protein sequence ID" value="CAD8205691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XW53"/>
    <dbReference type="OrthoDB" id="292412at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="528"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8205691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="566" mass="67664" checksum="E94D1AC01400D943" modified="2022-10-12" version="1">MQKSWETLCKNLRSPFLQHFLNLQTLIDELSQQIDQPQISHEIAIQIQEMISQIVQIIDIEHQNLLDGFEQSVLLFAQIDSEDRVKQFETLNLIQELFNKLALSYFYNDDHIDEKKVQMPLNDGTSTCSDFSDDNFEVEDFEAQVAQELFQQIKSQLQDPEIEQKCYQFQFTIFQEELNIMDFFNDSLELQIKEYDVIVFLNYSGLLQKLMNYKQDNQLEFFVGQLYIILNRIKNDEIDSLIEANCFELKYSRLSQRKFLRCKILSNLYTKLVYEKLDRWRNTQHIQEIQGENLKALRRFIVQHKAPLSRWEVSILSIQDVQVNNENILDKFQKLVIALSLPYDLLLENFTILDKLYVNLQQFQKWFKLSSIPFTVSEISQLFKEVSQTYGYYTNQGIKVIQRIDFYLFLETVYALNQNEKSIKEKPDIKSIKYNDEITIQQYNRHLQLKVNDLQLQLNQKNIIIDQLKKSQQEVQQTSRINTKSNNEIKDNESEIKIELLHQAEINSIKNETQQQLKIVELKWKNKTKDLLFELENKDKQIDQYRQQIEALMKELEWVKTERKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XXV4</accession>
    <name>A0A8S1XXV4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1430095</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XXV4"/>
    <dbReference type="OrthoDB" id="21225at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="154"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8206230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="295" mass="34020" checksum="427C042A0500E8BB" modified="2022-10-12" version="1">MEFQYKKKSIAIKKEIKLSLEITNHLINILNDYEQHLQITSSDQGTEFKFKLIIKENKTTDGSLEQFTIGFMLKQKSKKTVKNEINNDQTHNNSILIKPKSLSLFNSSKVIREPSANTPIIYPINQPKTFKTPSTLKRVRSSFANRILMDFDQKILIVDDEPFNHDTLILMMKNMGYKQFLKAYNGQQAIDLVKEKQGEISIIIMDLDMPIMGGMEATRILVDMMLDQKLDYIPIIGCTAHDDKETIDKCIQVGMLYVVMKPVFVKTLREAFQQITNIEDGRKRSLVCFTSQLQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y1N3</accession>
    <name>A0A8S1Y1N3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1460130</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000146">
        <property type="protein sequence ID" value="CAD8207255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y1N3"/>
    <dbReference type="OrthoDB" id="5337378at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="321"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="352"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8207255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="520" mass="61962" checksum="67F43523C8F8742C" modified="2022-10-12" version="1">MNIMEKVALGKYIHVERLIIQIQQFIVLKSFLQIKRFITQPSKVKLRILLKQQISELDKNSLYQINLVQIFDIFYEGYFIYIVMEKCDEDLQIEFDKMNENNEWYTEEQVLDLIQKIITGQHQLHYLKIIHQDIKPENILIKYENKGQVNERKIYKISDYGIGRLIKELTKKNDLIRTGTPNYSAPQMFENHIATEKSDIFSLGVLFYQICHKSQLPYDCSSMQKRYQSLQNLKTNSFQAPLLPYNKGNIFNNLIQRMIVYREDERIQFQDIIHHSVMKARGIEDTVVFNNESTSFQSEKFYDKSQYLEKRRVDQSDYKAYWSYFIIDSNFVIILSLKLIMMRIFLQLKLNVYLIAIYQLQYALALIYIRPFEMHLNTLEENDILILIDKLSKIQKAIKNFQAEDVTKNKYLEKQIQEEYHKFKKQFYSFSNILHSKFLNKTSFEDSQFFHKALMSKIKLQVLVSNITKLKKTMSGMLSKDLQDLLDQIIKLETLFPVNTYKSDIDLTLLFSINIQQTDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y287</accession>
    <name>A0A8S1Y287_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RING-CH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T1470016</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Lysosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000147">
        <property type="protein sequence ID" value="CAD8207317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y287"/>
    <dbReference type="OrthoDB" id="305150at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16495">
        <property type="entry name" value="RING_CH-C4HC3_MARCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45981">
        <property type="entry name" value="LD02310P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0391">Immunity</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="207"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="9">
        <location>
            <begin position="80"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8207317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="342" mass="40268" checksum="15FC2821C92B87B1" modified="2022-10-12" version="1">MNKDDKSFKQEKIDISESQVAPLDIIQIIQQKYARRVNQCNDQIIINEIPESSKNIDSTLQKQDSQQQLIFQKDIYARDSFKSVGCICRICEFSEYKDNPLIRVCKCIRSQKYVHEYCLKKQIIKKYRNNLHQAKCEICQDTYQMELKIEKIFAPKTAWSQSKDKLPLFCLLVFLIVMIVVVILLGTRLTEANQITDRKNFLDSQSFLIIFVIFAVIIILILLWLIAMIVKGLLIVERILFWQLQEFKPVKKSILNKNILISELRQSFRKSKLFGEQETMQNRTNKHQNTFNIQKINHNQDDQFIDVGEISTQRNQPKQVTSNVLTIQTDPRQTKRSRFSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y2W5</accession>
    <name>A0A8S1Y2W5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Small nuclear ribonucleoprotein Sm D3</fullName>
            <shortName evidence="9">Sm-D3</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">snRNP core protein D3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T1310073</name>
        <name evidence="12" type="ORF">PPENT_87.1.T1490093</name>
        <name evidence="13" type="ORF">PPENT_87.1.T1500090</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the snRNP core protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000131">
        <property type="protein sequence ID" value="CAD8202507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000149">
        <property type="protein sequence ID" value="CAD8208015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000150">
        <property type="protein sequence ID" value="CAD8208336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y2W5"/>
    <dbReference type="OrthoDB" id="309485at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01721">
        <property type="entry name" value="Sm_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000002">
        <property type="entry name" value="Small nuclear ribonucleoprotein Sm D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027141">
        <property type="entry name" value="LSm4/Sm_D1/D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034099">
        <property type="entry name" value="SmD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23338">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="10">
        <location>
            <begin position="5"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8202507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8208015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8208336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="122" mass="14202" checksum="8C5768DED2BB95AA" modified="2022-10-12" version="1">MSLGVPLKIMHEAVHHIVTVELKTGEMFTGYMAEAEDTMNVRLDEVQMVTRDGRPMSLEQVYLRGSQIRFVVIPDVFKYAPMFKKIRANAKSKNMQQIREKARQVREELVPRIKQGLEQQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y338</accession>
    <name>A0A8S1Y338_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1510001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000151">
        <property type="protein sequence ID" value="CAD8208446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y338"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8208446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="194" mass="23352" checksum="42905538EC7A2E17" modified="2022-10-12" version="1">MHDLLEYQIRFAKTMSKNEMTQVQYMQQGFYIKMKEQKTNRQMNFFIMMIKTELIKKILEVAHFSNKKFQIAADLYKVLVHSKNFDTQIFNIIIEILSKEENQNQDCLEFLKILKQNYAQNNNQIGNLSLLQKQKQLDAFEQTINQITRLINIIIKHDFHSINEFYTGTRQYIIQKIYQVVRIIEFLKFLVHSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y4Y9</accession>
    <name>A0A8S1Y4Y9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1450158</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000145">
        <property type="protein sequence ID" value="CAD8206992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y4Y9"/>
    <dbReference type="OrthoDB" id="10350613at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8206992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="97" mass="11507" checksum="09311D01B33F4404" modified="2022-10-12" version="1">MATFETMKFFENTKTIHQIALEYFKNESIQFLGNLMKTWEQSFENQNGMCKDNLEFCQTIEKLSDETKKIEVSLNSTPQFKKYSLKKTEVQFLKDSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y688</accession>
    <name>A0A8S1Y688_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1520056</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000152">
        <property type="protein sequence ID" value="CAD8208833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y688"/>
    <dbReference type="OrthoDB" id="299122at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8208833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="210" mass="25166" checksum="26E29290EC204072" modified="2022-10-12" version="1">MNFLRPPLTSFIKTLADPQLESEQFMIGNESITSKKDVIRQLQEQILYFKEAHNFTQSEIEVIRQFESLLESKHKRRTNEIHYHSQITDLTRKRKSELFRMKRAKKRAKIKVYAKKLNAARNRFRILGQFISNEEIQIIDETFGSHVFSDNHNFNLKIGRALKNKFGYEYYDKSQVIKEIKRLKRQQDQILSRIQGKKQIFCIKTKQLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YA32</accession>
    <name>A0A8S1YA32_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP synthase mitochondrial F1 complex assembly factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1580092</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000158">
        <property type="protein sequence ID" value="CAD8210273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YA32"/>
    <dbReference type="OrthoDB" id="16535at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010591">
        <property type="entry name" value="ATP11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13126:SF0">
        <property type="entry name" value="ATP SYNTHASE MITOCHONDRIAL F1 COMPLEX ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13126">
        <property type="entry name" value="CHAPERONE ATP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06644">
        <property type="entry name" value="ATP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8210273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="189" mass="22888" checksum="A15E5A9C66CF6B80" modified="2022-10-12" version="1">MLNNFLNKYKFAFTYPCPRKLREIVKMSLFERESKDQVISLWMEYHKEKQNNVAYVVSKDEYEILKRNTKESPLFLLPIKRKGGHFQLIGQSQTNSILFTFLEEYKRSGSFSSPYFILTIFEELLSQKQVALIRGDIMDYKIDKDEATFLTNQFLQFYMTPELYEKYIYTLNHKQHEFNYDDFKNHFQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YDG6</accession>
    <name>A0A8S1YDG6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1620105</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000162">
        <property type="protein sequence ID" value="CAD8211228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YDG6"/>
    <dbReference type="OrthoDB" id="282931at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8211228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="102" mass="11851" checksum="5D68FE1B8AD57C99" modified="2022-10-12" version="1">MESAFIFGCLFGMGIRMTVSRVIRQPLLYKCHQYPKYMLYTGIAFSGFDWIRRLSLETLCEKEELSEFLVHTARINQLATGAESVGDYKKEFVQIAVDEHIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YDK5</accession>
    <name>A0A8S1YDK5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1650081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000165">
        <property type="protein sequence ID" value="CAD8211870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YDK5"/>
    <dbReference type="OrthoDB" id="303690at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="624"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="843"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="942"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1177"/>
            <end position="1204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8211870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1235" mass="147076" checksum="7F65D2AEE0766968" modified="2022-10-12" version="1">MIYDFMLILTLTQSLQTMINQLANSLLFKNYQSVSFYQQYYNGYLNYRSNYNQAVQFLYLHRQHQMHCQILRCPCKNDVRKLSEKEISQIINLYYKNLLQQKFSEENALQYILILIDISNNTIQAHQTILNQKKNVQNGYILEIFSYLEQVLQNKLQTEMQQKQQVKFIEEMRLLKFEGLIVQQELQKVIIEKYKFYESLKLLSIQNFQKLYDSLYKLKEKVQDLIKKSRDLKIYDNKQLNNIFALRICHLISFLTQNLREQIDNQMRIDEIIQNEIYREDEFTNIAFTSNFAISLVASLNEKESGEITSKITLKQLQFLELDEHFNLNSVSQILHPSFRASHIKLVDQYLQKGKSRIIGTQIESMISPDANTIIPVKMILFPYFPKQNLNKYQLIAYLYKINQKNNAIILLDYNFNLMCSDKQFKQLINQYQLQLNQINLFQMIPQLRENLIYFHSSPNNAKPLYFQNQIIQIMSRFTRFYNNNKSCNICKDLDSFKQLEKLEQFSITSVNSISVDKISQVDYEISLQTIQYHTEDGLEYYIYYRLLIDLTQFDSEQKQQIHEYQEENKKEFIKKKSKSKSKQNDNVVRSLHSRQSTATIVKSSDDLLVYISQKTLPTAIKQFMFLSLINMCISIIGLVVIYILLLQKRNEQDICFNRLYYGTEFLDYYGIILKGSRHTVFYRDFNNFIPYNQTINIDQDIQVSITTSDKISFTLWIYKENCQNLINLYENYKTVLNSYQDEKLINFKFVDYQVYTIQTQVIQTQAAYYEIMNQLFYLAIKTFAGDPSLYLKGTLDTFQQINTRSILFLNFNDVCDITQEFIEACLQNSQEYNNAFDQQVQIIFYIFYIGILVFYVILMLTLAQIIYKIKQILQIFQRLDNRDIEDELQRLEFVAQNVKNEEFWFNSKFFQSFYEMSQDVSKTQTQGAQNIKLDDQFNLPMYYIMQTIIFVLLIIYFVIFQIQYNSYSNQINPIVNTALSGIQIRLQFIKFINNWDCYNFKTFYNYYIDVNENTPNINGNIRGDNKILQLLELDKNDIIQSYLNLVQEPFTIITKDLPDNDKASLLLNDICLLVGCDTETELFKQRFLVKEISKFFNVGLIQLYQQTFLATQNLGLNDNNETKQEFLKDFLDVLFSREYFMYIYWGLDATNFQLRLFSDYFIQIAKDQLTSQTEMIVILLFSLGIFISILLFSINGLLIHQIIRNYFSIRYSLRFIPMKVMINKNIIKQIKLII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YDL7</accession>
    <name>A0A8S1YDL7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Vta1/callose synthase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1610062</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000161">
        <property type="protein sequence ID" value="CAD8210917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YDL7"/>
    <dbReference type="OrthoDB" id="311985at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044538">
        <property type="entry name" value="Vta1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039431">
        <property type="entry name" value="Vta1/CALS_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46009">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VTA1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46009:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VTA1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04652">
        <property type="entry name" value="Vta1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vta1/callose synthase N-terminal" evidence="3">
        <location>
            <begin position="9"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="257"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="48"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="162"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="219"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8210917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="359" mass="42573" checksum="66E7227A26AE532B" modified="2022-10-12" version="1">MQQNPEFKQIQALIQHSQQLGSFNITFGYIAKAYVAQKLHALYKKTQQQNYLQMLQQLINELEGLKAQNPQLQNKEENKKHYTEFVYKFFYDADTRERDGEVSQELLHMFGSVQKSIEVLQYFEPLTQDQQEKQKYAKFKMVEQHKKIKDPTLIKPKPKDPIEEELEQIAQQQIQKSQHQQQNQIQQQQQQYQDKQAINQQPLQTVKPVAPQIQTPQNLPQQQQQQQQQQQQQQQQQQQQQQQQQLLLQQQQQLKQQQQPQQPYQPQQPQQPQIPQLSQIPQQPQSINQQQIQSQQQPQTQQGVTQINKLTPIEQSQTRNQVKSVLQQAISEVDFKKYQNAKDQIQKSLSMIQQLLDSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YEF4</accession>
    <name>A0A8S1YEF4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1710067</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000171">
        <property type="protein sequence ID" value="CAD8212866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="290842at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035734047" description="EGF-like domain-containing protein" evidence="4">
        <location>
            <begin position="17"/>
            <end position="3351"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="467"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="775"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="875"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="926"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="1176"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="1237"/>
            <end position="1274"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="1301"/>
            <end position="1333"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="1543"/>
            <end position="1573"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="1604"/>
            <end position="1641"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="1668"/>
            <end position="1700"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="1749"/>
            <end position="1778"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="2060"/>
            <end position="2088"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="2316"/>
            <end position="2349"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="2382"/>
            <end position="2410"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="2438"/>
            <end position="2471"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="2474"/>
            <end position="2504"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="2916"/>
            <end position="2949"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="2952"/>
            <end position="2982"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8212866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="3351" mass="387444" checksum="92FF02430E0B01A2" modified="2022-10-12" version="1">MFLLGLFIGYFSIIDAQWRIRYEYLFSNVNFQERIRTDQMNYPFVHFTSSFMIPTQFDRTANFISCTNPETSYITLNSQYPQVQQYYCEIRKHFYSSSYYYDHYQSSFLKGFLSFDLYFQGTWTNGKVTFSIDLFTGEYLYNSPSTYALNSQFCDEIAYDIRTVNFTIPYNVNGDFLFGIQNTNENGQVSIRNIYQMYYEIICYPSCKSCTGPKQNECTACYFGTPTNNVCLPCPSDQYYVKNVGCRLKCDSIQPLCFNGFCNACPSNFQLGYVIVNQKISNDPNSYKDFLQWSKIYDPKFIDNSVDVFHYTKNGQNILLYGIFKQSSGIMRQVSISNVWGYYMKVKIMIIIFNGFPMDCGIQFKFMESSFFYFIFLMQLQQKTYEFDNNTLVGSIYKNSSGIQLHKFKEFQETSLGPYESYSSSYKYIIDGVFDLPKYTILFSAIGNFSDTSFGWAFQSIWFGTQRCTNNCQACDVNFKCTYCSSQTFRYRDGTCISTCNQPHQNIISDYCKDLDDETPYSQLLIREDVNHFELDYYPEYTLVSQRGQNFLKGPDIYFSYLKGLRIFGGQFIWAQARFRRVHQNLGPHHSISIGFYVFFGPLFPSDGKFIYQIDQQDPLYTSTLNAVTFQNGTKQQIIRKKINHSLNHLILELECFGENNEVQQAYCGFYNYYLAIHYCQPFCLQCTDEKTCTNWNSTYNPSLIQFSQDECQTNQYFDVYQLLCVNCEPSCSTCKSKTVCLTCINPTYTLTKVGCFCKLNQIEESNQCVNCPIECNQCSSSSYCTECLSINNRKLLNGQCICIDGYYQKDQDIICSLCDKFCGTCFGPTSNDCLTCNIEVLNIQLIESTCKCPTNSFYQNSYNTCTFCHTTCLTCLNDSIDGCLTCDSQQNRQLQGLKCTCTTGFYTQNNICIQCPNNIDVSLLECYKYCDNGDLIWNTNTCNTCGQGFNLISNECVPICGDLLVVGYEQCDDGNNILDDKCYNCQFQCPINCQTCDINTILPCPDICGDGLITGDEECEDGNDIQFDGCFDCKYQCQTQCTKCIKGKCSECATLGWQIDVSSEPWLCKEVCGDGLRVGIEECDDANFYDFDGCHNCKFLCRIGCSFCDYEQRKCLSCEFEGFQPSDYFCQPILNDGLLVWDPYGFFYERAHKSNRCDNCYSECGSCGGVFFKFECQPLECTSCVNKKCQACISGQTLSTNNICQPICGDNIKAYNEYCEDSHVLPYRGCQNCRPKCQDSCSKCDIIYKDYQYYGIGCLQCKDGYKLNDFLCYSKCGEFLFSYDKECDDGNLIPDDGCHFCQLNCQKSCLFCIKGVCLDCEEGYELIQSRCHPIINSFEQYKYSNXDNGFNLISNESVPICGDLLLGMSNVMMVIIFQLINVIIVNSNVQLTVKLVTQTLYDLVQIFLEMDQLLAMKSVRMEMTSNLMVVLIVNINEVCGDGLRVGIEECDDANFYDFDGCHNCKFLCRIGCSFCDYEQRKCLSCEFEGFQPSDYFCQPILNDGLLVWDPYGFFYERAHKSNRCDNCYSECGSCGGVFFKFECQPLECTSCVNKKCQACISGQTLSTNNICQPICGDNIKAYNEYCEDSHVLPYRGCQNCRPKCQDSCSKCDIIYKDYQYYGIGCLQCKDGYKLNDFLCYSKCGEFLFSYDKECDDGNLIPDDGCHFCQLNCQKSCLFCIKGVCLDCEEGYELIQSRCHPIINSFEQYKYSNDFKCLQSHKNWIMKNSNFGQNDVNNYINLQNSSCKFCLINCEICNEESCISCVKGYYLNEQQQCQSECGDGILTQDEQCELNEQYCFNCKYIHPQYCQKSINNVCINCDDGYYFDELEKLCKLSCGDGGDGILIYNEKYQIQSKIDCKFQCSEDCLVCKNGFCQQCKDSFNIWNGQCHKVEKLIRSQLECKEHIQGLCIQCYEQYVLNLQGECIPSCQDSCVICKFGQCYECKESYYLYQNTCYPENECSLYFISDFSVFDITCRNLQSIEIQRNNQQSLDSDEISNRLQFLFGYDFDLIDSQFFENWLFLNKTLNGQQIQIVEDEIIQINIDNNDSDSYFNLQELNCNTDCLLCFDTQCIQCQIGYLIVDFSCVSNCGDSLTVGNEQCDDGNIYEYDGCYECQYQCQKYCEICERGNCIKCISNFVFDSLQQCRQMEQLSITQNQDCRIIQNNQCILCEHGNLESITGICILDYYLENQCIGHCNICLNQKCQQCEYGYYGTYCLEGDGSNLPIERCTDFNQQQSQICKQFQECNQNCIYCVNKICYLCNEGTYLFNNECIISKIDKVAYKSDIEPKCGDGIVQIYEECDDRNTKQFDGCFYCKFSCDTNCYDCFQGICQTCQDGFILNQNHLCDPLCGDNFVVPFSIEQCDDGNFKSLDGCYKCKYECLSNCLDCNQILCLKCEEGFETNKSYCVPICGDGIIIQQFEDCEDLNDEPYDGCYECKFQCRQNCKLCSQGVCLDECPEGMIYVGDICMPKKCIDNCQICEKGDCFECNIGYSYDGLKNQCIQDKLSDSSLNVSMIEMDENLYICRLTECVFSPAPILQVILINQTFSRQYVEIFFDQQVKLKFDEEELKFQFNFTNLKQGDYIITLHQKQPINYEINFASYIVEIENLASINEKPFLQIFCSSDVINSNNQTLQNKFASIKLNYPQILSNEQKQTSQFMQNTNKVFMYGAISVSLVSLFAGDSSFFLETLNVLQQQSFLKFINVDYPENLYIYFEASDMLSVSSYFSQLNLDYYYNFISQKTEPLQVNGKFELYDVDPDLIISLLPQVFQCSGIVLLLFLSRLIYRLFHLIMSKKQIYDYFNSKSQFLIKSFLTILNTLRIQMKNLINLRRNLNSNYIKIFFSLNAWDILFKSILQIRYTQFKQAREYIQLIGSFLLIYTYGKRGSQRINMILNQXFEDCEDLNDEPYDGCYECKFQCRQNCKLCSQGVCLDECPEGMIYVGDICMPKKCIDNCQICEKGDCFECNIGYSYDGLKNQCIQDKLSDSSLNVSMIEMDENLYICRLTECVFSPAPILQVILINQTFSRQYVEIFFDQQVKLKFDEEELKFQFNFTNLKQGDYIITLHQKQPINYEINFASYIVEIENLASINEKPFLQIFCSSDVINSNNQTLQNKFASIKLNYPQILSNEQKQTSQFMQNTNKVFMYGAISVSLVSLFAGDSSFFLETLNVLQQQSFLKFINVDYPENLYIYFEASDMLSVNSYFSQLNLDYYYNFISQKTEPLQVNGKFELYDVDPDLIISLLPQVFQCSGIVLLLFLSRLIYRLFHLIMSKKQIYDYFNSKSQFLIKSFLTILNTLRIQMKNLINLRRNLNSNYIKIFFSLNAWDILFKSILQIRYTQLKKLGNIFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YK18</accession>
    <name>A0A8S1YK18_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T2340003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000234">
        <property type="protein sequence ID" value="CAD8214655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YK18"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8214655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="258" mass="29257" checksum="A6E02BA020B04646" modified="2022-10-12" version="1">MPIQINGRKQENQQKLNQIQYKRCNNQQRNKVRFNMQSIQAKEMNYEVQKAKIQWQIEGVIEGTLGRQTVDFIMDVIDAQKLGKGLFVSASIQGVCILKVQQNLLEEQFHIVGIGLTAFMKSISVGGVILSDCLSQSEKVYNFYCSENWKCYRIRQCGMELGAAGGPIGVVIGEIIGGFIGEVSGNLLGKAIDHFTQFNLQIDFNQNKKSKVEDGYLAYLGTPLKISWKYVKDNIKSLILMAQTDTHMAFLRYWDLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YL03</accession>
    <name>A0A8S1YL03_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T2260001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000226">
        <property type="protein sequence ID" value="CAD8214569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YL03"/>
    <dbReference type="OrthoDB" id="406844at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="250"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8214569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="459" mass="53789" checksum="A00DA29AFF45D026" modified="2022-10-12" version="1">MQQPKIIENEKDFCCSKGHKLPVVTIALDEKLSRNQRLLCTECLENIDIEGKAVGLKKMIQLIEENQVKKMENVENIKTNQIKLIESLHSIVDQMKSLELEFIILKSNQTDSNIYIQEIWKTNLCWTSKFNPKLEQFNQFDAYNQCQQLLLTVELGSHKSTQNDQQQQINIENINQEIQSVNTKTNIPPSQTNQSSNQLNVKPFNYLPIQEVTTLNFMQKSKQFISGSLDKSIIIWKQNQNKQWSYQQKLNRHKGFIQCLIINTNEDLIVSGSQDCTLKFWINKNEWLCQQTINDHSKEVYGLSLNQQQNRVVSCGYDQYILIIEQSEQNKEWKVIQNITIDNYGFRVCFIDNNMFTSTPIMNNNNKYFTITRDISIKSGSDMYASFPQQYINQISILVSKNGEYVNLIRKKQNEEILTEQYIDFGIDSLHGTMSNDGEYLITWDYQSKQIQIRRYQEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RZE9</accession>
    <name>A0A8S1RZE9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ERV/ALR sulfhydryl oxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0020357</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RZE9"/>
    <dbReference type="OrthoDB" id="17199at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016971">
        <property type="term" value="F:flavin-dependent sulfhydryl oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039799">
        <property type="entry name" value="ALR/ERV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017905">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12645">
        <property type="entry name" value="ALR/ERV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12645:SF0">
        <property type="entry name" value="FAD-LINKED SULFHYDRYL OXIDASE ALR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04777">
        <property type="entry name" value="Evr1_Alr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51324">
        <property type="entry name" value="ERV_ALR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="ERV/ALR sulfhydryl oxidase" evidence="2">
        <location>
            <begin position="82"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8133346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="204" mass="23951" checksum="33FF7F2899DE2545" modified="2022-10-12" version="1">MEKNYIVLIGVSIFFLILFMVNTFNQYEQVVEQIELEKQPFAIDLMDVKVPSDWDIVKPEKEELENMDEDEIEIINEKKFSSGITREELGRNGWTLLHMISATLPVEFDEKFTFKINVFLNLFGQFYPCKECAGHFLNMTTILPYEGNSRVDFMQYLCMLHNEVNQRLNKTLFNCSIVHERWGGDCGCSSKLEESIKKYSRDQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S414</accession>
    <name>A0A8S1S414_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0030078</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8133990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S414"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47026:SF2">
        <property type="entry name" value="FLAGELLAR ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47026">
        <property type="entry name" value="PIGMENTOSA GTPASE REGULATOR-LIKE PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="254"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="107"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="217"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="255"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8133990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="282" mass="33768" checksum="80970DD5A69CBA31" modified="2022-10-12" version="1">MSSFQRPTSSRKNTNTLPPQQGDLEEYIQILYDHQKSCEKAGKYLEADQAKKRLAELKKELDSKNKVDVKDRHCSEKQEIEKAHLDEFNQFNTFWDQKMIEFDQEAQKVKEQTLQRHEDELRQFSEELENSIPIKPKDSAELLALRKTEEQLAKQENYMEAHLIQQKILAQERDELEKWTVGRQQKIRNLISQLRQKQINELNALTQRILSGQEEQRKIRSQELEKLLQKYQNVRKELESQQVQEITRLDKSCKNQSIMQQSRMNQSRMQQSYAKDENQYIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S4C8</accession>
    <name>A0A8S1S4C8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">asparagine synthase (glutamine-hydrolyzing)</fullName>
            <ecNumber evidence="2">6.3.5.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0030283</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-aspartate + L-glutamine + ATP + H2O = L-asparagine + L-glutamate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12228"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.4" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S4C8"/>
    <dbReference type="OrthoDB" id="409189at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00712">
        <property type="entry name" value="AsnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000031">
        <property type="entry name" value="Asparagine synthase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006426">
        <property type="entry name" value="Asn_synth_AEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050795">
        <property type="entry name" value="Asn_Synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033738">
        <property type="entry name" value="AsnB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01536">
        <property type="entry name" value="asn_synth_AEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006949">
        <property type="entry name" value="PRK09431.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772">
        <property type="entry name" value="ASPARAGINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772:SF2">
        <property type="entry name" value="ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001589">
        <property type="entry name" value="Asn_synthetase_glu-h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="11">
        <location>
            <begin position="2"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR001589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8134392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="589" mass="68572" checksum="7D98A5C1A16E76DC" modified="2022-10-12" version="1">MCGILAILNIRGTEVEVRAKAYELSRRIRHRGPDRSKIIVLEISPGLYHVISHERLGLVDLSDKGRQPFQLMDDENIIFMQNGELYDYWDLRPELEKRYRFSSNSDTEIVGMLYKEYGAGNFWNHLNGMFATVLIDMNKKSFQAGRDHIGIIPLYYGYDKEGSLFLSSELKGIHDQVMDVKQFPPGHYIDESYEIKRWYNPTWHNFDHIPTGQINLEELRDKFVEVVRREVQGDAPFGLFISGGVDSSIVAGIVAKLIKKGEIDLSKRGMRKVHSFCIGLEGSPDLHFAKKVAEFHGFEHHSFTYTVDEGLDYIPEVIYHTETFNNTTIRASTPMYMMCRRIKALGIKICLTGEGSDELFGGYLYFHKAPNRVEFHQELIRKLHDLHKYDLLRANKACLAWGIETRPPFMNKQWVEYVMQIDPKYKMINAFEPQMEKYILRKAFEDLEHPYVPQEILWRQKEQFSDGVGYSWRDGIIKRANQLITDQEFSQASIKYPVSTPRDKEQYWFRQIYTSYFPSESSVLTVPYARSIACSTEKALEWDEAFKKNTDESGRAVLAVHNDAIKELIQPDEDRSTEDISKVQEHFQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S705</accession>
    <name>A0A8S1S705_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0030529</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S705"/>
    <dbReference type="OrthoDB" id="346907at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13990">
        <property type="entry name" value="STKc_TLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000698">
        <property type="entry name" value="Serine/threonine-protein kinase tousled-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974">
        <property type="entry name" value="MIXED LINEAGE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974:SF23">
        <property type="entry name" value="TOUSLED-LIKE KINASE, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="337"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="31"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="116"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="246"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="37"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="88"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="366"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8134839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="613" mass="72144" checksum="309FD18034BFD86C" modified="2022-10-12" version="1">MHQQLPPEQLDRLICLEARIHKKSKEEIIQEQFLQMESEKQTDENDRNKQNMDSNKKSDDLKCSQNSENSPQKLFTSQNLSPVQKKSIITKKHSQQKKKKKTKEGQQKDITQYLQYQKKQQKAKKCEQKLMEYSIQDSPFKNKSPLPSDPVLEKQLKEKQNQVAISEIKIKELETHNSELRETISSLTEQQLRIQDVVKQFAIENEKLKKQQLQIQLQTNRVRLGEYVVQREKTALVDVWIDGQELREAKEQSKRLEILKAEYENKKKNSKNKEEQEMRTLNVKLNFLIKEEQQLQETVDRLETEKNLHIKQLKRVYEEEHARFTKNNDYPLIGERYQVLSLLGKGGFSEVYKAYDLQELREVACKIHQLNSNWSDHAKQNYIRHAIRENRVHKELNHPHIVKLYDSVEIDKSSFCTVLELCDGPDLAYYIKKYKCFPEKEAKLLIGQIISAIKYLNNHKNKIIHYDLKPQNILFHLNELKISDFGLCKVLEDDNSKLQLTSQGVGTYWYLPPECFHMGDQPPNISSKVDIWSIGVIFFEMLFGQKPFGQGVSQEKILKEQIIVKSQSVTFPQKPIISNECKEFIRGCLAYNQIDRLDVHQASNHSYFQKKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S7D6</accession>
    <name>A0A8S1S7D6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mago nashi</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0040529</name>
        <name evidence="5" type="ORF">PPENT_87.1.T0040530</name>
        <name evidence="6" type="ORF">PPENT_87.1.T1120118</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mago nashi family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000112">
        <property type="protein sequence ID" value="CAD8196420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S7D6"/>
    <dbReference type="OrthoDB" id="6495301at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035145">
        <property type="term" value="C:exon-exon junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11295">
        <property type="entry name" value="Mago_nashi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1560.10:FF:000001">
        <property type="entry name" value="Protein mago nashi homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004023">
        <property type="entry name" value="Mago_nashi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12638:SF0">
        <property type="entry name" value="MAGO HOMOLOG, EXON JUNCTION COMPLEX SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12638">
        <property type="entry name" value="PROTEIN MAGO NASHI HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02792">
        <property type="entry name" value="Mago_nashi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8135996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8135998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8196420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="151" mass="17747" checksum="95AA425EF5AE2A2E" modified="2022-10-12" version="1">MQTNQNDEFYLRYYVGHKGVYGHEFLEFEFRSDGRLRYANNSNYKQDVLIRKECFVSESVISEFKRIIEESEIIKENDSKWPAPDKIGRQELEIKLGNAHISFTTSKLGSIHDVQSSQDPDGLRVFFYLIQDLKCFVFSLIGLHFRIKPVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S8K9</accession>
    <name>A0A8S1S8K9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0040363</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S8K9"/>
    <dbReference type="OrthoDB" id="305726at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="109"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8135700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="332" mass="39738" checksum="19D47DBADE9A605B" modified="2022-10-12" version="1">MNQYDRIRPIVFQKATEVNYIRSNSLAIRSSPLKQQLDQSFDLQTNQTKNQTNQTLNYKQLYVSESCQNNNKENQISGKQFSFRVDETSFLKQRIKQLETQNTNHISENNKLAHVLDQQIQQHQQLQEQYQSKNQIIRKIDDVQKMNKYQLTNNTELKQINDQLIISKQVVNNLEEKVQFILQDNQKLSEENDKYLFQDQQLKIQLEKYKSRCQILESKLKQAEDESQCLELQKKIKKQNDEIELLQKENNDMKQQLDQNNNIIQLQEQNKSSSQNIDKYKETIQELECENQYLLKVIDIDQQKMNNLEEKLNLLTKENQRLTDIVKNRHKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SBT5</accession>
    <name>A0A8S1SBT5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0050232</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SBT5"/>
    <dbReference type="OrthoDB" id="306465at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035893523" description="EF-hand domain-containing protein" evidence="1">
        <location>
            <begin position="17"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8136602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="102" mass="12036" checksum="066D5B4EC4A7C168" modified="2022-10-12" version="1">MVRRVIVLLLLGIVLGLQPYEADFLMFDQDGDQMLKYDELRAQFPSQNPYNTADFVAQFFLDYDSNGDKIIEFKEYSAKREQQKLELEQQQQSISSMYHDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SC46</accession>
    <name>A0A8S1SC46_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0050278</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SC46"/>
    <dbReference type="OrthoDB" id="299394at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8136692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="102" mass="11884" checksum="8A05FD74F6D46943" modified="2022-10-12" version="1">MNDSLRSLSTNDSLEGQTRKKKKSNATSKKTNRPQNISYTEVVDLLNPSNLFINKNLEEEFNAIELIPETSYFPHFKFILDENLTCTETKWRRLPTFIKLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SDP5</accession>
    <name>A0A8S1SDP5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0060325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SDP5"/>
    <dbReference type="OrthoDB" id="284439at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035947606" evidence="1">
        <location>
            <begin position="19"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8137850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="307" mass="34336" checksum="BEDF0268C853B0F4" modified="2022-10-12" version="1">MKNIQLILLIVLFGFASADIIINKSKRCECPSFIKTECDKWYDCKWAETSCQKKECTDYTTEDKCNGECQWKGGKCIDEKKECEDMPTEESCSNFAGCGWKDNKCIEFTQCSDFIVTKAERCSVLQGENGERCQPKGVSVTTLFYKHLAVAAGFQCENKVYVDCSKFVTEATCKGDATATAKCQWKSDGKCYAFELKTCRDADGFNQMCDPKYCKKDGQLCVNRSCSDITTQALCTSLPKVDSNKSILCKWGADNKCTTATDATHLNEQTCNDVTFGSYHWATNTCQQCASNWILSIMSLIILVVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SEC7</accession>
    <name>A0A8S1SEC7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0060465</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8138130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SEC7"/>
    <dbReference type="OrthoDB" id="300737at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="74"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8138130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="516" mass="60998" checksum="4D483F9A4B9524F0" modified="2022-10-12" version="1">MSKDYKQLAIQYHLAYMNLKQRIDLSQRAFNTIDFKVQLEVYYQQKLKSVIDKYEDHKYDEVFQYIITNCSNKYQAQEQLITDMKNAREEFSKNINNYQQQFLNLHDKKEKSINKLKLAQQKLVQSQSFYVSITKVFERNVLTIELVNNNVDQKLGHMKTIQDLMKNKENTFGNYKIAVEKFNQQYNEILPEMELFVNQIKELESNVAECIRQLFQKIALAEVSALRNRQYDLDQVIKYLDKVDFTKINTVFKFTDEAYAPAKVEQFQTFSTKIVNNYLHKFNFVGQDEKNLQDQEQQQQEETLLQEQKQKIEEFSTTLQNKQDLSEKLINSIEQSINQNTAYTILHSLSQLIKAPVSIGKRNLQMIQSLFNKLLDFIPKEGPNIHKIQKLSTFIYSQQEEVISLFQILCQNPIFSKKTDYWEWLIFWQVNQTCEGVEDSDRQLIIFQQFQNTVQQMVQAKIPVQIINTFTSDLAPYYGLKAQQIEEIKKLLSKFILAQQSNPSSKLQIQSFDSVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SER4</accession>
    <name>A0A8S1SER4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0080185</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SER4"/>
    <dbReference type="OrthoDB" id="294198at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8139611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="341" mass="40405" checksum="C258807A17998FF5" modified="2022-10-12" version="1">MSSKPKYLLKKVTKTNDQQNNMFMTQTLDQDEAQFYKDGKICSYYIVGNPDLFKKKSFILKQSEQSNIPSKPSQRVIQKHQRHNQDSDFIYEDMDASDVEKEVERVIQQQKQPEQGVKMALTKPDQIYAHRENKIMVAFEQQQQKWQQRVIQSAKACGRDPKQSLFHHIDAARQRYEDRKLLDLIQSDSEKYGNHLWEMQLRAAPGELNNKNEKAVFEIVRKNKAEQVYERPEGFKKRFEEKKDVIEKIIPNLSVKELEIVGQNKLIKESISMLNLAKNQKNVKFNNEIPLNSKRKIILSAPFEKKKQKEEPLYYQDVLEMNYNKRDLRQSGQLDIYKQIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SIK7</accession>
    <name>A0A8S1SIK7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0080205</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SIK7"/>
    <dbReference type="OrthoDB" id="346371at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042410">
        <property type="entry name" value="WBSCR13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44321">
        <property type="entry name" value="TRANSDUCIN BETA-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44321:SF1">
        <property type="entry name" value="TRANSDUCIN BETA-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="252"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="62"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="62"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8139630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="474" mass="55908" checksum="245377F4F7D2811A" modified="2022-10-12" version="1">MERILFEYTSKIIWPEYNYMKILIEFSVIYILYTIYKVSVGKYINYKAYTKITRIEKINTEDEVKKQKQKTQEKQDKQEKNQKQGKRKNSEKEQQQQSKDVQKQQNKLETQQQKQENHPHISYKELLKQERHNKLLQNKEKSKSSNKITHPLYLSSVKGFSDYITDFAYNNRYLIATGFDNSIKLFEMEQFFKNPDQPKYLFHTLNDMHATAICISKKEEIAYFSANNQLFNLEFYPKAEDKQYFKVIPKTKNCHKSDINSLHVDENDKILISTSSDTHIKIWNMKGDLLKEINTSYGEHYSSCFMKGFLVVSSWSVDTSIYEIKFNKDHTFRTLEKEEVLRGEKTSVLDASLNEHGTLATLCNKSQCRVYKINTERKIKEDAKLLTKVEHPNISAAAVNENILVVAGDGSIHVYNRNFQQIDQIDNAANGAYIKRVRIHELHSSLKNKNFYVCFVWGQDEERVNVFDFTKYHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SIS9</accession>
    <name>A0A8S1SIS9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD domain, G-beta repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0100028</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SIS9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="213"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="258"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8141271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="476" mass="56202" checksum="018544B3F43D6AD2" modified="2022-10-12" version="1">MQIRCTQTSNHSQQINVVCIEKDCQNLRFCCPSCIFQHAHHLISLISLERLNEWIKQRIETIFVVQNSISEFKLELNDILNLNKKQLLEIGDSRIEVLIKGFWKFDDLVRLQFSLLQQSIYNIRQIVKDVTKCFKDGILQQQSFNQSKGLLNPQIQDIKLKDFNQPMTQIVQNRDQCRAIAMNKDCSIVLGGYDQYIKVFEHKIGKLKQTQILNEHSSNVITLNFMKKSNHFLSGSCDKIIIIWQMNQINQWVYLQKLIGHDSSIFCLLINTNEDLIISGSQDKSIKFWQKQIKWQYSQSITDHIDIVRSLSLNEGQDKLISCSDDQFILIIQQSLKDKIWSVVQKIQVDYNGFRLCFIDENLFTFQPYCKQQMYVYEMDRNTKLYWKIQDIKVNCGSKNCCLFPQQYIKSKCILVNKNGKFINFMRKKENGEFFNEFSIQFEVDEIFGQLSDDGKYLITWDSYSREIKITNYKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJN8</accession>
    <name>A0A8S1SJN8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0070410</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="302310at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050505">
        <property type="entry name" value="WDR55/POC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44019:SF8">
        <property type="entry name" value="POC1 CENTRIOLAR PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44019">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="64"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="106"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="148"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="193"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="231"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="273"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="409"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="451"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="493"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="538"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="576"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="618"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8138982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="701" mass="79367" checksum="F542D029EFACD038" modified="2022-10-12" version="1" fragment="single">MEEPKGIVRHIQTLQDPQLSRSYRGHEKTYCLSFDPQMRQLASGGNDSIIYVYSFKPQGRPFKFNGHKGAVYSVQFSPDGQIIASGGEDRTIRLWKNSVLGKCTAIKGHIGSVRSLSFSQDSSMIVSSSDDKTIKGWNVLKNNFMFSLAGHTNWVRQAKLSPDSRLVVSGSDDSTVRLWDVNLSKELQKFKCEDSIYSIDWISDGTTICAGQMDGKIKLWDARSMRLIQYYECSKKSVNTINTHPSGNFLLSGDDESNLKIFDLRQGRLAWSLYSHSQPIKQVQFNYAGDYFASAGLDSNVLVWQSNFDENMKPCSLNYIQSQQIIKQNXIKYYIQLFIQFKFKLMEEPKGIVRHIQTLQDPQLSRSYRGHEKTYCLSFDPQMRQLASGGNDSIIYVYSFKPQGRPFKFNGHKGAVYSVQFSPDGQIIASGGEDRTIRLWKNSVLGKCTAIKGHIGSVRSLSFSQDSSMIVSSSDDKTIKGWNVLKNNFMFSLAGHTNWVRQAKLSPDSRLVVSGSDDSTVRLWDVNLSKELQKFKCEDSIYSIDWISDGTTICAGQMDGKIKLWDARSMRLIQYYECSKKSVNTINTHPSGNFLLSGDDESNLKIFDLRQGRLAWSLYSHSQPIKQVQFNYAGDYFASAGLDSNVLVWQSNFDENMKPCNVINFVDKNLSNNDSQTLSSSNQFKVPQTTFKANKQQNKXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SQR4</accession>
    <name>A0A8S1SQR4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0100141</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SQR4"/>
    <dbReference type="OrthoDB" id="297711at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8141487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="401" mass="48095" checksum="7ECEB311468A7C1C" modified="2022-10-12" version="1">MYRFSLQSPNQILVTPEGNVLLTGIYIKYKKEIFTLSIFNPPETEELDIEKLNVWKCGILLYILLKGSLPNKFKYLYPYSVKDSNKSILFDSEDPTIPFLQRKLLIEMLNEQPEQRLTYNQILHNQWLIGNSNNVTPRQVINREIIQFDKYLLQCIQLDQYLKEFIETYTYLNIRHESILQSFSKFDLHKSQKILLKDLNYIKQVLKNEALILSILHFSESKNSFIYEDCLQQWENEIIMLKIENLQPCFPNQEVKKQQVVNYLQPKRKKYEDGLILSDTSQMIMNLTKDIYQFNEFIQEVQKGIKEKIKDQMNNQQVFIKSEEIKQEFNELLNLLKSDQEYQTNKIQLNEIKQIQQILGNNNLLNLVFQKEIVQHLEQFEKQDQQRRYKQLLNKMINKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SRW7</accession>
    <name>A0A8S1SRW7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0110267</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SRW7"/>
    <dbReference type="OrthoDB" id="305335at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035852423" evidence="3">
        <location>
            <begin position="19"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="168"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="363"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="168"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="187"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8142648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="426" mass="48240" checksum="C6D727007E452D3B" modified="2022-10-12" version="1">MKVFAIMALLLIAAYSQTNEIDVVLKMLADLKNGTIKQLQELESDWATTQYQKQDIVNDLQRSASTQRGECNRRDQEWANKERDIRITLSYINWLEKRIKENNERLQRLDVNRCESNANFINDIKNSKKTLNLIAFLRRAVKNAKETDLPTLLQSAQFIQLKSFLAEDGEEPEVTSEDNEPPHVYDEVTETPEETSVTEDVEQEVETSAAPEEGVEAADHEIEEAQQVDETEQPVPEHPLEAEEKATNEQLAKEAAAFNKIIEKGQKAPAQGGSGKDYSQFTVAQQELLNFLDILEDQVRGSFGNKQDNQVNSAMGYSDFKGLITKENETFRGHLAAEDVNLEKLQNQLITILQATAACKDRLKKIQNSIDLANEDLANAEAHFKSVSETLQEEQNTFDDVYRIYSSQVGSQSTSYKRDVQSKITN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SV94</accession>
    <name>A0A8S1SV94_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SANT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0120048</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SV94"/>
    <dbReference type="OrthoDB" id="272624at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039467">
        <property type="entry name" value="TFIIIB_B''_Myb"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15963">
        <property type="entry name" value="Myb_DNA-bind_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SANT" evidence="2">
        <location>
            <begin position="39"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8143117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="137" mass="16217" checksum="DB8E71166A5DACC1" modified="2022-10-12" version="1">MASLSSESNTKDSETLKELLKQRPRIQKKQQQTKINKSNSNPIWNPEDLKKLEKCVSILGTDFDLIQLLFKNRTRKQIMNKYKKAKIQYDRKHKEKFLRIKEALNKEQEVQRHRLGSIDSIDLNIALDIHELSTKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SWA4</accession>
    <name>A0A8S1SWA4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0120353</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIN1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SWA4"/>
    <dbReference type="OrthoDB" id="313535at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031567">
        <property type="entry name" value="CRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008828">
        <property type="entry name" value="Sin1/Avo1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031313">
        <property type="entry name" value="Sin1_PH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13335">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX 2 SUBUNIT MAPKAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13335:SF1">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX 2 SUBUNIT MAPKAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16978">
        <property type="entry name" value="CRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16979">
        <property type="entry name" value="SIN1_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRIM" evidence="3">
        <location>
            <begin position="166"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="SIN1-type PH" evidence="4">
        <location>
            <begin position="395"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="74"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="74"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="102"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8143686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="516" mass="60350" checksum="E087A383801AEE8F" modified="2022-10-12" version="1">MEISNRKDIEKLIAYSKSIQVGLIEDDFNDQYTPPEKYVYDSEPIPEIKLEELETNLNEDEMLKLLKQNVQSAQMKDSISSEPDSLESQDSIERRATPPKRQQQQVQPPIQVPVQKSQTTVDMTEYNKVPEEVLQFLDGAKGRKSMLGAAIKNTKGTSMLLPSQGKPIKIYFFNTQQYIQINVAKNTTVLQVIRMSITEYQNSKNFDQKLLRYPNNVDAYEIRFVDDDFEFKAEMAFGAIDKKKEILSTQTNIFSLIEISNYKEQTNDMQIFEKINPNMVLIEVVIIDQKPEKNVTTKVQLDKNSIVADIITQISKKNKQINQVNFLIQLNDDDTILDLDLRMPVVQLQNKKIQIKQKTWADQAEQQSINTQRRDTITEQDDYVDAPLNHIQAFKYEKFTVVKINERGKRQDRILGIDQFRIYNMNKKENLGLFERILNNKNTGTKFPERLIEDIVDIRIANQFLYITFKQENDFKELKFDTLDSKIALKIYKKINFIRDFQITTQKVRTYSQKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SX29</accession>
    <name>A0A8S1SX29_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0130317</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SX29"/>
    <dbReference type="OrthoDB" id="302214at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PH" evidence="7">
        <location>
            <begin position="255"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="430"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8144623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="738" mass="86509" checksum="352EBF166C2D12D2" modified="2022-10-12" version="1">MDLRWLLDEDNLTTADQCDHLNISFISSPNRSQLGKTIKNQYSAVEFADTLGKIFQAIKEKNLEKVQNLVNIHKKRSYNLGGDPEFKWDIINDLSLNWSPLHHSIFLGCEAIFLWFLSMGGDITSITYDGYSALILAVLSRSELMVNLLIAQPNLNINHISRKGSALHIAIQQNQISILFLLLQHPNLNLNIYNDKLQQPIDIATGIAKIMLEKELQLRTLIYQNQSKSQISILNQEDSQLNTLNTFIINRPQKPPVYKGYIEKLHFSHLYSYQRYIVIDPDTGVLIRFENKENYPLNPKETIPLQNIYNLQIIVKDRLFIDNSIQLLIEYNQKKIYFLFSTQKMAQIWYDSIKTALGYSKYIKYKLDQYKQKKEGKKVFNQVNSMLLDMNNQSIEIDTLTKQDQYKEEIIEFKQKSEKQLFNNLKFSDFQILEILQKELIGKIYKAKYLKDNKMYVLKQQHKEKLIKRSQLDQAISKVKVLRIINHPFVINIKGLFQTKDNFYYQMKYYENGDLSQYIGQGNLLSENVSKILIAQIILAIEYLHSLNIIYRDLKPINILIGKNGFLRLVYLGHNNDILNDRATLSLNRFPAYLAPEIIKGEKLTQQVDIYGIGVLLYEMLSGYPPHYIQNTEQMLERIQYCPINYDRIKSKMSIKILKKLLQKDQTKRPNLKDIKNHVFFADINWNKLLIKNILPPKLIKNNRQQFISEQKISEIIIDHDYIDDGEKPNYVDNWSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SXJ7</accession>
    <name>A0A8S1SXJ7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0130442</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SXJ7"/>
    <dbReference type="OrthoDB" id="293384at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="148"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8144870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="478" mass="55656" checksum="A8AB80D8AE7664F3" modified="2022-10-12" version="1">MLPKETIIEQAKIIILQKKPNLNIEKFMKLDFSLLCWQTVVVKLTIHQFEDGLRQYIGSYFTLVEGKAKLQRIQIENQQVIQELELLTDQPFYYGLDYLANDKEFFQILALSHCTLTIIKDVRIQQIIKNYYSLCEYQVLESLSKVYFLQQEPSVLISMLIHESSRIKLQKGQPVYKIGDSVKGFYLVLEGEICIKKLVELQTQSQDEISNTKKSRKKLVIQNILSKDQQFGEMELFMKVTHRNELAECYSLECDLLFVPTHSLVEAYQTKLQDPHFSLEKLFEQFQEYINLKNSKQNEAITKYIQVNKPRVPPKPEMKPTYPLFMNLQPIQSFKNPAEKVQPPLQIIRTSSMYTQSQSQTKKVNKQPESVFVNYSLAKLNRQDGLMTVSQYIENIKLQSTGKSGISAYQQKKKLKQNALSVVTEDQQKLIDYVRQLTPLYISKKRNSVGLRKSLPTSIYQSAGKQSYMMLKPDFSKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SZ11</accession>
    <name>A0A8S1SZ11_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WDR59/RTC1-like RING zinc finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0130269</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SZ11"/>
    <dbReference type="OrthoDB" id="288435at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061700">
        <property type="term" value="C:GATOR2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016239">
        <property type="term" value="P:positive regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16693">
        <property type="entry name" value="mRING-H2-C3H3C2_WDR24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037590">
        <property type="entry name" value="WDR24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049566">
        <property type="entry name" value="WDR59_RTC1-like_RING_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46200">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46200:SF1">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17120">
        <property type="entry name" value="zf-RING_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="275"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="WDR59/RTC1-like RING zinc finger" evidence="4">
        <location>
            <begin position="702"/>
            <end position="748"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8144516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="749" mass="87315" checksum="66F8CCA5DCB23CCB" modified="2022-10-12" version="1">MDQELDLQTSISSIDIDYQGRNLVVGGREILKIIDLDQMHKKMNIKQNLRRQGTKLAIGYVEFNRKKPTLLLAASVQGQFQVWDIDKQKETVYRNHQAAIHRMTWHNENCFISGGQDYAINYYDYRQPVAQPIFTLQRNEAIRDIKFNITRDNYFMAASETGQLEYFDIRKQQENVTEINLRGRKQDPFTNLIICLDWTKDNVLASGSNDKEIKILQVENHTDIKEKEVIQHIDGSSHIKWHVQNNSLLSVSSLNRDISVTTYDIRTPFRPMHIIKGHNDIITSFVFNQDEQYVFTGSKDKYLRMQSVRQAYQVYNNCPIFPVAFNQYNDLLMKLESPFQNQNESDKINHYNMNLQLNQKQSSNEDFNLYKSKTQNTSQNNLIQNSLQDIQKATSGTPQNSQSQKNNVRSIFYIEAIDQRFDPKIPFDEELKYFQKNYNSKKDFSYNSNIALQIDKPEIAHYWKALEHLVNDFKKLSVECGPNISMLNCPIQISNKGNDQSDDDQKSPLEQMTQVTKNNKQHEIKQLDSQDLQKLYKYFEKYPAAFRQELDSKNIVIEDQNHLKIHIERIPYYKNLLRTMTPIGYLLTDKKHMMQVISNSILQLIQHVIDQGDLPSGFWMAACAKNYVQFPDLIAKKWTQSYIELLVAFGKHTLAMKAALESPVEQFRTSNKYVTNDKCLGPCKNSQITGAFCNICKTDFICVICFKPVRGLYLWCQQCMHGGHMKEMKNWFSNNTQCPSGCGHNCFDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T0G7</accession>
    <name>A0A8S1T0G7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0130257</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T0G7"/>
    <dbReference type="OrthoDB" id="306495at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8144494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="236" mass="27829" checksum="0E7D32850AADAF98" modified="2022-10-12" version="1">MGISCSSQKSAKYQKSKQTQQQQNINKGKSVHKVKGSKQKDIFIADDLCFILRRLDQLHQSPNKHKKNGTISSLFEKQQQLLEWKLECDIAFIIDLTMKENKKRLRLLKELLSEKERHQQRFGIVYFGHNKAQFDLIDDCIETTYDLQRQLQTLKLDEWINIIKSLRNQIVTSKDRQLSQIYILSEQKQENIDQSILQIVDNLLEDTCTKNSLSFQCHSYKIEECDSLKQMELSFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T179</accession>
    <name>A0A8S1T179_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0130216</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T179"/>
    <dbReference type="OrthoDB" id="290533at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053083">
        <property type="entry name" value="TF_kinase-domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44305:SF2">
        <property type="entry name" value="SI:DKEY-192D15.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44305">
        <property type="entry name" value="SI:DKEY-192D15.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="23"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="394"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8144412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="508" mass="59644" checksum="207B0CAD455AD866" modified="2022-10-12" version="1">MGNKVYIIKSADNSIFNQNQSLEDQFESVGGQSFQHIDRVELYRLKRENTKYATIFEKRHSNTATDDEGPLLELHEQLMLLKHNHLVKYYGLCIDPNLTSNLQISRWFYEAVYKTLKQVCGHHFSNKSYIPERQLWKSLHEILLALHFLDARQKWHTHIQPDSIYLDKNDSIKLIPKGVLRLLSGYQVVLTGKGQALLSPQQMEGLRQKLEIPIHDIQKSDVFSLGMTFLELMTLKDSFECYNYDLHNPYIKDQILQERQLDIQNIGYSTDLVRIVLTMLQYEEMDRPTFLELLNSHEVIQNLENSRNNPLQPTTHTPKQELKIFQNRFSSDSSSLQIVQPSIPITNNQQQIQSSPIRIQSQVAPPQYLPIHNPKQQEVDHQRQEQILREQQIQQHLQLQLRQQQQLLQQQQQQQQQQSLLIEQQSQQILLYQQQLQKLKNLAPQPKIKEQQQPKLQDYMIHPLPIPPIVLDPNARNSTVDEFQESQELNNRINSALALSRQAINKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T4A6</accession>
    <name>A0A8S1T4A6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0160283</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T4A6"/>
    <dbReference type="OrthoDB" id="674948at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14811">
        <property type="entry name" value="bZIP_u2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47416:SF8">
        <property type="entry name" value="BASIC-LEUCINE ZIPPER TRANSCRIPTION FACTOR E-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47416">
        <property type="entry name" value="BASIC-LEUCINE ZIPPER TRANSCRIPTION FACTOR F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="7">
        <location>
            <begin position="127"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8147250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="354" mass="41659" checksum="531A7BC9B0E59BC9" modified="2022-10-12" version="1">MKASYENIPTDTLIQLINSSYELDQMTNKWMFGEEDHHLVSSPHNDQIRKSRLQGKHNEQIIMNNQDLFNVNYSKSVYVNKINSLIEIAPTPQESSFAIKKDQKAYIKAKKLFEKDNGYYQKEQDLDDSNKIKLAKNRQSARDSRKRKKIYVELLEMKVAELSKQIEVLQKNLDQQSLFINHCVKIPSLISDFNLSYQNQFQNLSKNITKRQLQILDEEFGVNSEKRDYLCNVIFNTLVDHILPFDLKKTIDAAINNLGIFKVGEQQEIKQLIEFQQQFTQHYKKFELAAYDMAKSYEYIKREAEGLEKLKLQLIQLLGPQKYVKNILDIQQLQQQIIVKEEDINQKGHTKFEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T5X5</accession>
    <name>A0A8S1T5X5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0160247</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T5X5"/>
    <dbReference type="OrthoDB" id="294148at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8147178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="168" mass="19946" checksum="A4FCA5B9E750AA5E" modified="2022-10-12" version="1">MKPNDLLQEFTDFRRKLKADIQKTEFSKSPSKNYTKTQSSPQKPNSGFKQKINFSKHRSQLQERCNTISSTQNQNYYSQIYNPQFQCITHEKFNSQQQNMQSLKSSKFTNKKKQNITDRILSYTQLNNFCFTLQQIQDEDIKELPNEYKQLLIQLAQLIQEKIGVNDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T6I4</accession>
    <name>A0A8S1T6I4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0170299</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8148100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T6I4"/>
    <dbReference type="OrthoDB" id="309407at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8148100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="219" mass="25612" checksum="474B68998D63AE6A" modified="2022-10-12" version="1">MQNPNFMPILLYTQPTGYPQYYPMYYMPIMPNYMIPQYQSSQEPEINQIISKSSDQSSFSNQNFDPNNQLNFQSIKQNNNEIAVENNEEIQTNTESNKPKQKSGKKKINLLQKSTNIQKNYAKAIVSYACGQRVLIINTLGKKKGIEFLKIINQLKNKLKNINHIKNYTQKESFLSMFRILGNKFLKNEAVSYIYHSNIQQKSCHLKHLKMIEQNLLKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T9Y4</accession>
    <name>A0A8S1T9Y4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cilia- and flagella-associated protein 91</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0160362</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CFAP91 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T9Y4"/>
    <dbReference type="OrthoDB" id="567787at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026720">
        <property type="entry name" value="CFAP91"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032840">
        <property type="entry name" value="CFAP91_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22455">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 91"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22455:SF10">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 91"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14738">
        <property type="entry name" value="CFAP91"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CFAP91" evidence="7">
        <location>
            <begin position="71"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8147402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="634" mass="74410" checksum="6CE08EAEA9B3D21E" modified="2022-10-12" version="1">MQEAYVLHDQYRNQSMQNYISGTSRPLYFKRPLVPTMTDTPLKLSQPPVQQIKEQVLPAKVTIERVKDVEIQTDYRDAEAQTDPYTPAHDQNDDQTIEILALQHLKWDKGLPASIEELESIEWNREKVWFENSLPPISDEASFKLRRALMKSQEEREWKKKETDIKITQNERLYLLQEALKEREKDVEEKNAQRIESIKIKKTELKNRMVAKIQKRKIKILRKMQKSRKEIDNEDKGREIIEDYANFASKVYAGITREGLSLDKIASKYEVQPVALNTYKGLTELSTTIKPSILETTVNVAQFIKTIEKDYTRLEIQHKIEVNKARNQIFGTNKQDQSNENQADHIGNKNIIIRPATPTYNQEFENGDIKRQEMKFENYEQFKEEATKRQGEDKRNSAVVLLQRLFRGRAMQNIMYEGKEKRTALIEELLTVAKIPDLPEAEQEEILMQQHEEKVKNAALEAIQGEVIAETLDMLSKELLRIKQEKKIAQMMKSAEEDRRLREIQEAGTRQAEQILRDREDVQYNQIMRVHQGTVDTYLHWIFKNTIEQAATRQATIMTNLRKQKMNAPVEHFERKYNSDETIIKDLVQQFLIPNVQRSKLQKQIQIEERRFNEAAKKSIQATLSSAAQELNQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T9Z3</accession>
    <name>A0A8S1T9Z3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL22</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L17</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0160373</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T9Z3"/>
    <dbReference type="OrthoDB" id="304509at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030684">
        <property type="term" value="C:preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00336">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.470.10:FF:000012">
        <property type="entry name" value="60S ribosomal protein L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_uL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005721">
        <property type="entry name" value="Ribosomal_uL22_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01038">
        <property type="entry name" value="uL22_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593:SF10">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="158"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8147412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="184" mass="20815" checksum="4D3C88E2BD2E0BEB" modified="2022-10-12" version="1">MGKQYAREPAVAKKSCKAKASDLRTHFKNTYEVARAIKGQTLAQALKYLQDVLQHKRCVPFTRFNGGVGRTAQAKEFGRSQGRWPEKSVRIVLSLLQNLAANAQVKNLSNEKLIINHVQVNRAQKGRRRTYRAHGRINPFLSSNAHIELWAAEKDENVKKETNNQQVARQSRKQVARSKLSIGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TH51</accession>
    <name>A0A8S1TH51_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase M14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0200227</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the peptidase M14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TH51"/>
    <dbReference type="OrthoDB" id="10253041at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050821">
        <property type="entry name" value="Cytosolic_carboxypeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040626">
        <property type="entry name" value="Pepdidase_M14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756:SF11">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18027">
        <property type="entry name" value="Pepdidase_M14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M14" evidence="5">
        <location>
            <begin position="182"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="493"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8150372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="590" mass="69074" checksum="46790748D8EEBE9B" modified="2022-10-12" version="1">MKKLLKTNKDFGNQYLDFTQLFEKKLKQPTINNDCKKDLIDQYRQRNFKHEPQPFYESSEIIVYGSFENSNIMAVQQEGNELNILLNGDTNTNGCTQWFYFGILVKQPQQLTFRILNNRRGKSLLKEYNHIRVYDNNKWNSETTKELYYYRTNINHPFYHSQDSSIANLQQNFPLYTLQFNYNFTQNKNIVYFALTLPYTVSNLYHLVYHSALKHKVLCSTPLGLPIFKLKTKNKISKVVIILARQHPSESVGSHICEEIIKLLDAGHQVQNKYRFIIFPMLNPDGVFLGNSRCNFNGIDLNRKWDMPNQTTEPEIYNVVKHIKKYKVAFLIDLHGHSKKLNQFLYGCATPIKHISDYMRVKQFSRLLQQGCDLFNYLNCSFSVTPDRMSTARVAMWKKFQIANSMTIETSLYGALKKPFEKEEFQLLGQNILNALYQYDEYQSDPHLYDTQEINQAIKLIKKDGIDDKESGSDSDAEQDIIVIRNSRLKVTSRRDSTPLMTKSKPKQQSETPIQQSTLFFKNEQTYSFKRIPSYQMKRKVSRMNSFQSQIIDEPLSAQLPTIYNIPSKQYKGTLDLIQAIQVKSLSRQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TIC9</accession>
    <name>A0A8S1TIC9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0210043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8150842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TIC9"/>
    <dbReference type="OrthoDB" id="308246at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="63"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8150842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="374" mass="44668" checksum="F405C77E17A8650C" modified="2022-10-12" version="1">MSCDCVHLRSELENIRNKQSILLKDHQMQLELIRTKTKEQYESLLETQKQGFIGVQKINEQEINKLKEIIAIKNAEIETLIQTNSKYRAQLNQEEQISNLKIQNLKKESQDILSLINKYQEEINQLKQEQPQREDLLNEQHQLRIEEIQNYYTKLLKQHGKEKQEVLIKIDELVQKEKKLNEQINNQLSQIQLLKLEILDKTSILDQNEKDIQKLKVELLKLQNSQEQNLNENQLQQKSLQNNYDVIVQEYENELENQKCQILQLRSSIEQLTIKYNELKEETYKQNLIIQSQNNTIREQEALLDKLEIHSQTNNMQFSQMMENQKKRLEQAYSYQLENLKKSFQTQIILLQQQQADESLMGRQKLIRRSIADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TK29</accession>
    <name>A0A8S1TK29_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0210414</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TK29"/>
    <dbReference type="OrthoDB" id="320070at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002895">
        <property type="entry name" value="Paramecium_SA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01508">
        <property type="entry name" value="Paramecium_SA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8151552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="376" mass="44096" checksum="18CA811F25B625AF" modified="2022-10-12" version="1">MNLFGIQAQTRQSVRITLVIMFHYQQSYKMIVIHIAILLLLISLFEFMMGQQEDTAQLQINYKGELSAWNETSSQWADKSCQTAPATSDYYHDSNCRSYFINKCTVSQYGQGCVIVPASCTTMTENQYYFNKNEDPSCWTWKNLCNQIMRQFLQTQAKLIQLDMHSILLQMQSQQRLLLFYSVKKYYLHVAQMENIFLLEGLAFKLKVWLFMLFHLLDNFVNGLLLKGIVKQKVALLLPQLLKKCANYFTKFSTKIDGGFISKSTCAGALIEAGRKIALNGTVCFWDEQLKNIDILRISIETSCKSLIWDIDQKCRLLIYNQRISFFWYLIVFRNLQKWRMRTGYDDAFIQSLNSLDPHSLLSQQFIYIFYISQYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TNM4</accession>
    <name>A0A8S1TNM4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0230309</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TNM4"/>
    <dbReference type="OrthoDB" id="10251809at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093:SF18">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13415">
        <property type="entry name" value="Beta-prop_FBX42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="4">
        <location>
            <begin position="615"/>
            <end position="649"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8152922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="662" mass="76914" checksum="278D91E4FE7923DC" modified="2022-10-12" version="1">MNQGKIYCYGGSIDIEGTILLDDFLEIDVVQLKFRQIKSAPTGRVEHSMCMYRGQILIIGGRTQKKIFNDCKTYTIGSDKWNQIEFEPAHRFGHQCTVYEDTIIVTGGSDGQIILDDVWLLVDLRTWIRLDIKNPLPIFRHQAALAMKEYLIIFGGCTFDGKRCNDNFYALNIVTLKWIELPKLSRHPYPRVQHTMLCLLHQSREDILIIGGLNYQDLSILNFSQMANLVDLQPQSSLMSYRSHTVEREEFLQDIPLEVQNIEEGTSFLELGKYYEWKIETNQEIKFTPRTGHSVVQCQENLYLFCGSDDTTIVNDMHCYNIFKKQWEQIAPKGIAPSPRSGCKGVAHQHDIYYFGGYTNRRGEYFNDLYVFDTKLRQWNQIRTTREIQPRVDMSLVINNEKLYVFGGADGSNRFNDLHCFDIQNNQWVKLQTHGQIPTPRFGHTAEVYKNQMYVFGGWDGFKTLDELYTYSFASNYWYLEKVRNKPPSRYRHSSTIIGYSIYIFGGVDAAMTRYNDLYEFNCELKEWKFIETAGNTPSARTFHQLCSYETNIYLIGGNDGTKKNNDIFSDLSSISQLEIQSTIIPKENGLISILKNQVNELGQRLREEQEFNLCQICQSKDINSVFLECGHRFCCYECSNKLELCKICLKKPNRIIKIYVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TT10</accession>
    <name>A0A8S1TT10_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0260349</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8155173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TT10"/>
    <dbReference type="OrthoDB" id="190265at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035760643" evidence="2">
        <location>
            <begin position="23"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="4">
        <location>
            <begin position="64"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="3">
        <location>
            <begin position="145"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8155173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="360" mass="42290" checksum="B04BE0F6F547AE63" modified="2022-10-12" version="1">MIKYWFFLFFNNLILINQRNEANNLELTVYWNLSIHKSFKCVRDFKCRNEMNNEHLNEHDQLIFHNWMFEYGKSYDNDFTVTHGMQIFMRNKKNIEKHNHIGVKYKAKLNEFSDQDYDELALKMFMHLDFNDDDLKFCNHHFFSKQDIKELRNHPILNQIREQAKKGDSLDWSYTIQTIRNMWKLERKTQLNYQRLILIDCCVGDKNNGCNGRSPIGAYKFINENGALKKNEYREYDATQQDCQKSGGNISKGQIVDVEKVNDPTVEQIKEALQDGPVTALMYADKSWFEYGGGIIDTCSYVGAIELSHAVVIVGYGKDHYQFKNSWGSGWGENGLFQVQRNGAPECLDKIKKISYQIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TY30</accession>
    <name>A0A8S1TY30_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0300323</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000030">
        <property type="protein sequence ID" value="CAD8157745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TY30"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8157745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="110" mass="13521" checksum="FE9DEAFC4CFE9506" modified="2022-10-12" version="1">MIINHLILIIVIKKIYFHNIHAVLKFQSQFVLKQIYYRLPIAFNLKYQQQDEVKIKQSIRIHLATKKYFCLNQILLQNKYFQIFQLHIKILQYQSQIKTFKLYHQISKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U648</accession>
    <name>A0A8S1U648_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0350055</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U648"/>
    <dbReference type="OrthoDB" id="294336at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053083">
        <property type="entry name" value="TF_kinase-domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44305:SF2">
        <property type="entry name" value="SI:DKEY-192D15.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44305">
        <property type="entry name" value="SI:DKEY-192D15.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="417"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8160575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="476" mass="54959" checksum="667340D9BDCB0719" modified="2022-10-12" version="1">MGAEQSVAQGIVKSKNFTILGYKENEIYGSFKLIESQSKQYAMVSKTCQNYEDYTNLCQILKETQSLNEIDHLVKLVEVDTQEETNLCSTFYRVTALYEYYDINLRQITKQASINCLFVLKCLADILSQLYGHNIVHGNLTPDYVLIEKIKGEIKLNNCTQLTGYNHYKRILSGDSLWYLSPELLTALGKKELRPQYNHEKNEVYQLGLIALEFWGNCNISEIYDKQNYKMNENIIQESIDEFGVRNGYVLKNVIKQMLQEDPNARPTMTELSQSFKQLFEIQQQVEEPKVQNYEEQGNQYEQTNNYQEINKETHEVQDEVLQFNKESFQYQEGTKRDIQNENSPRVQFEESPKKMEKQNVDFNSQSVKQSINQSIKEFYVTGSIAAQFGQLNLLDSNSDYKTIGITQLQDDGDVLQEKSQNHPTSPKGQDSIKPKKSVKVNTASANKKGVSIKRPKHEIMDTKLPKQAVVVSKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U839</accession>
    <name>A0A8S1U839_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0350283</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8161010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U839"/>
    <dbReference type="OrthoDB" id="291382at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8161010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="94" mass="11358" checksum="AC907367B1503490" modified="2022-10-12" version="1">MENKQSLQIHINLTQFIQKQNKEEQSAKLYEDWHQLIKLKLQGSKQDDTRKLIYGRLSETQQKSRRLTPNQTIQKDQDIIKDIQNIKFRLHKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U9J2</accession>
    <name>A0A8S1U9J2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0350034</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U9J2"/>
    <dbReference type="OrthoDB" id="248233at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000264">
        <property type="entry name" value="GTP binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050055">
        <property type="entry name" value="EF-Tu_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721">
        <property type="entry name" value="ELONGATION FACTOR TU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721:SF9">
        <property type="entry name" value="GTP-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="2">
        <location>
            <begin position="240"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="633"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="636"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="663"/>
            <end position="674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8160533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="757" mass="87125" checksum="537F175E13FD50C3" modified="2022-10-12" version="1">MQQDFQQQQTLINGIYSLWQGIDDEEQKRENKKPLKQAHPQFSQLTDKQKLRKQILNNNKKNKGKLKGEGQVQHEDFESNQTQIQQLKYETKPQFVLLVKPNNEEIQIDLEQYIDEQYAIQGNNSKVSYFKQESDFGHTEYKLRLVDPDPKRLTHLTTQMNYRLSEGNGMAYYKIGVEDSGNPLGLNKSDMLGSLRTLCSMAQSLKAELIVVGINQGTNGKTSEVIVRKGLKDGINLDIRILLLGESGSGKTSLLGVLSTGQLDNGKGLARMKFLKNRSEITSGKTERVSHTVIGFDSEGKIMNHQNQLNFNPTCMVEKLVDLSTKLITFIDMGGTKRAHNQMIQIINSQFPDYALLTISSLQPIGKPTMDFLKLIQIQQIPFMIAITHVDKMDEYFYMDKVEQLKEMITSLQIQSVPLVVRSEEDVVLFSKQILSKTLIPIFLISNLKQRTLDYFIKFLNLLPSTNELTNNSNLDSEYCIHNVFEINNQKVLGGTVLKGVIRVKQILQMGPDKHGRFFPIEIEEIQCNRVQVKSVQCGQICTIRFKQGTLTQQFGSDQNPVRRGMVLIDSKSNPQAAWDFLAEIWLFDDQKDAKKIAASFEPVINTQCTRQICKIIREEQLQFEPLKLRKQQSKSIDESSRQNQDRQQQRLPYRKQSEQIKKASSSSNLNKSYRSPRRKSSQNKESFVDEDLKKIDLFVISEKNDKPQEFIELQPKQPNFIRLKFKYFPEYITKGMKLIINDNGLQAWGFIKYINY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UBK1</accession>
    <name>A0A8S1UBK1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Importin subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0370264</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the importin alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8162228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UBK1"/>
    <dbReference type="OrthoDB" id="29145at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024931">
        <property type="entry name" value="Importin_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005673">
        <property type="entry name" value="Importin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8162228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="485" mass="55934" checksum="EAF6EEFC622D4AD3" modified="2022-10-12" version="1">MSDFKKNLYSRQKQQDLQLLKLIPDSLPDEIKQALKSSVENLTKQEADQEKFEEYIHKVNSDDIITSFIGLNRCRKLLSNPQIAAPTEQMIELFFQTRVHIKIFDIAKNTNIPLLKYEALWIICNIGFGTQKQIQTIVDNDGINILFQALESEYVEIKELGVWALANMAGENLIFRDMLLQQGVLKVFINLANKCRDNQSETNNWGTKNLTILKTLVWAFSNLAKGKKTPEKIFFKDLVYILCQIIVETEDEEILKDACWGLSYLSQEDFLMHIIIQEQITQKLVLLLQKENQSIVIPALRILGNILTGNEEQTNVVMNCGVVQVFQKLLQNNKRNIRQEVCWSLSNIAAGSSGQVKQIIRDDQLLNSIFLLLNNETSNVIEQISYIFSNCIFSAELEDIDYLVIQHGLFQKMSLLLDMNEKIVIRVTLEGIYQIQKRVQNDQRQEQYKKFIVDSKIMDKVADQLKYSSKEISSKASKVFEIFNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UFS2</accession>
    <name>A0A8S1UFS2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0400069</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8163798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UFS2"/>
    <dbReference type="OrthoDB" id="299636at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="10"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="161"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8163798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="256" mass="30272" checksum="33C7485673AF7E58" modified="2022-10-12" version="1">MDKIALQGEINSLKLENMNLKFQLKTLLEERQGALGNNEQQINYDERILQLQTTINELKNKCDDLIYKNEVLNDDLEYERNKSQNLTLFNKRDFSSPNFQEQINNLKKKLEVDFKNNNQYSDELVKIIGNQYETIVQLDLDKQKLLTKLKLQTNSGLIIQDEDDQLNVNDLKQNLEQSKRQIINLEQQLHEKQQSFDELNQKYEILFNQFQQQQQVLQKNQAKPSILDTLQKSIIQTQPSYDIHQSHFSKSIIQSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UH59</accession>
    <name>A0A8S1UH59_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0390015</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UH59"/>
    <dbReference type="OrthoDB" id="270720at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="23"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="62"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="319"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="37"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="78"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="322"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8163072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="346" mass="39328" checksum="ADE86576D245326F" modified="2022-10-12" version="1">MGNSCSCQCQTKGMDEVELNTNQKQLSQSKPNAKIDMGQHQEVDGKEKNMAHLKDEDVAQMAGARNNSIEDSSSPKLMKRENDDNKPEDRYVSLVQSTKGKLTRIKLSAIEMKNGGVYEGEWKNGLRDGQGKYVWPDRSFYEGEWVEDKANGFGKLVHVDGDIYEGQWLDDMANGRGIYIHSGGARYEGDWKNDLQHGQGVEVWPDGAKYEGRYENGKKHGQGTLTFADGSFYKGDFVENDITGYGEYYWKDGKSYKGQWNNSKMNGKGITQWADGKRYDGEYKDDKKHGFGIFQWENGRMYEGYWLNGKQHGKGLITLPNGEKKEGQWENGKRIKWLNADESPNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UXJ9</accession>
    <name>A0A8S1UXJ9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0490017</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UXJ9"/>
    <dbReference type="OrthoDB" id="444540at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051581">
        <property type="entry name" value="Ca-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34524">
        <property type="entry name" value="CALCYPHOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34524:SF6">
        <property type="entry name" value="CALCYPHOSINE LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="23"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="390"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="348"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8168299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="861" mass="100927" checksum="8F03D58CC47B7027" modified="2022-10-12" version="1">MDIQQFEKELVTKLQFKASSRIGVETILLKIFKFFDLNNEGYLKKQEFLKAIAKAGVTIPDQEFGERLWMTYSFDEQLYYKDFINRVVGKQQIEEQAESRIVQTGSPLEQLRLKLQLKGALGILNLAISLPNQFTVQQLQDYLKRNNIEVDGLYQLFNGEYMSASNLINQIKGQYLNQSRQGVVSDVFYTFEQREISPQQLVNRFNAERHPEIKILNRSPNDVREEFLSGLSVYLQLRGIKMLTIQDFFDFYSYFGFYLTNDEIFKTILVSVWDLNQLKQQALQYGTPPQSIKQQQLNYSPQQQNIIANPQIVQQLNFSPSQDKSVKSYQQNQQQLQERQSVPKSVKSHQSNVSQMSSTSRQVELIVQRIRNRLISRGARGFIQLYRVGKILDSDHDGMLNLTEFRKAIRDHKIDVTDPEIDIVFQYFDRDQSGLIDLWGFMFVLRGEMSQLRTQLVEQLFEKYRTNDYVSLQTLRNNFICRNHPDLKNGKKSDDEIIQDFFEPLQLLHNINGGFGHENITKEELLEFFSNYSASIPDDKYFEQIIVNVFRLLQDGSKNHHAGNKQVFEPDHKRGYLQDHHRFVLQGGSVSANAPFGTFTQQEQLAQSRPSVGYNQPASFNIFKPLEDVKVNQNGSQIQQIPLSQSQISQQSQQQLQYEREQSVKSNKQKMDNIKILRNKISQRGLRGLINIQYRFQLYDKSHLNALSYQEWKNCFKQWRLDISDQILDDIFQQFQTNGMMNYSGFIKQIQGTMSLRKFNAVQQAFESLPKTTIDIVKQQFNAKDHPDVRANRKREDDVLCEFIDTFEQHHIVFTGGDYVKNPNVTFEEFIGYYNNLNLLIEDDIQFEQHIQSVWNLRRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UZ08</accession>
    <name>A0A8S1UZ08_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0520046</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000052">
        <property type="protein sequence ID" value="CAD8169915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UZ08"/>
    <dbReference type="OrthoDB" id="10310416at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8169915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="287" mass="32460" checksum="A6C1546A94EC2B09" modified="2022-10-12" version="1">MLPKKKPLSHTLKQDYIPPENCIEAKSFHSVPAINQVNSENHKFLQHIINTSNNTLEGVQGQMQIQFENDQNTYVGSGTLCIFGQGFKSNKYFLLTCAHNLVDLSENHQSIKQANKITYYYSRRNYKTYFCRLLAENFVLHPGYFFNNKIDDGFDLSLVKLTKQQIMKDAPVSTNSFWGIPLEESGATQGSVIKIIGYPSSASGELYSIDGIIKEINFHDDGQALILYDILSACPGQDGSPVYLKMGDNHWTIVGVHLGYSLTKNSFIGTGLTKYIHKWIYKQQQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V2B8</accession>
    <name>A0A8S1V2B8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0500050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8168906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V2B8"/>
    <dbReference type="OrthoDB" id="305984at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8168906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="311" mass="37269" checksum="34F199A50982A127" modified="2022-10-12" version="1">MKNQIRNLEQQYFIYCFFHFDDTQYKFYIYIYVYFVLNNIMNVMDLLRDRAQDIRLLQNNQMAIRQIIETDFFNQLSQIKKISKNDLILLQDTMKFIIRIIALNFEINNLELNLSSTARIILELYQYFGLSSQAELLQSEKTNQSKQSVFLNLVQHLIDITRMALELTIKSQTNLRLFCWGKEIGEKGNTNNSKVAQLRRVQRSESNHQDKVYQSKKSIQKLETQQIYESIPKSQRTIEDIQHYSRDTSVIRNHHQRKTELIQNSKALPCIYQMKQNQLNLYRQLKQRNSSIHNSIQTYIRQIYDKNTIID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V6K0</accession>
    <name>A0A8S1V6K0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Leucine Rich Repeat family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0570101</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V6K0"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052394">
        <property type="entry name" value="LRR-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8172454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1158" mass="136733" checksum="2898C2EECD515397" modified="2022-10-12" version="1">MNQSNSKIEKQIISPKQQCMLPSLYCDYSARKKQKLKTYFRQEQCSSISPNKQIIKTQRDLLKQIDSERNSELYSFEDEVSRSFKFQRKSRLHQRNKILNQPIQNKKLLLTDTTQIETDTPYFKDKESSISQSNDESEEKNENKIDVKLEGETGKALKQISHKINQELNNSFYHFIKKSEKMKDLILNFQQKDNLSPIHYYCLNDSIFPKRIELNKQDVKEINLSDLGLTPKYIPLIRNLLSQKRDKNIKSIDISQNKINEWSLKLLIDVFPLKVKTINLSLNQLNRRGAVLLSEHLKNFENLRFLNLKGNQIGDIGTVTILEALKPSLQIKKLNLSDNAISDSISYELKEFIIKNQSLQVISLNWNQLGPIAGLFIAKGLNYNKSIKVVDLSYNKIGQSDNNYECIKEWCSLLSNTYTELIHLDLSYNQFNEKQLQLINYSILKNSRLYGLHVEGNKCLAHVDPLGFIQFPQQIKGQQSMQPKHQNIDGVNFIPMLNENENGSDCCWICQGWMEYHFIYEPEPEESKTDSIYLHLDFLDFKPISMTTSKELWKQISNKFNSTTVLEDLTTGEIIHQLKYAFSAGKIITMTAINDAYTEDNKIKDDMKSILSELNSKFFFTSYQMCPPNQKILYFFSNPTGKGIFINPRFPIIQLRSNENIELIMDKEKVFFYPDGSQISLSQVDQVNYFQTKQMFVIDQKNQYKPLVKVIPRCLDNKYFLKRFAVDASKQKSNLIYWQKEQSAFKSYQGDTQDVLDECFQFDWTCMGIQHFLKNEQEVEKVIEIMRSHYPKLKDIYKYYSSFGYNVNKQQNGYQVETFNIPLNLIYDLTQELLPQDIQIEEVQSQVNQIKYNCDSKFIYNPEKGFVRYQFIEFIFRIALLVTKQKQKPKQIVDEVYRIFQVLTERFAAFDNQQQWREERLWTKECGNLIYIKQGFIQRLHDYVACIKNKKWTFKLKWIALTEFIDFCNQMGFQQFLSDTQLKIIYNFAMQTHQDELTQDRHLRMSTVEFTEALARIAEFISPNENDINNISINRLILPLHIKLENLLTHIFNTLRILKVNNFENFSIYFSLNPIPILPKVHELQQDQYECTTQELRIYNTLSYLKMFKIPYLNPNYKQFLEIPFQLVQNQKQKRSIVERQMTQKQKLLKLKATLQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VGK3</accession>
    <name>A0A8S1VGK3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0630134</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VGK3"/>
    <dbReference type="OrthoDB" id="291080at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050656">
        <property type="entry name" value="PINX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23149:SF34">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_106, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23149">
        <property type="entry name" value="G PATCH DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G-patch" evidence="2">
        <location>
            <begin position="21"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8175593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="157" mass="18083" checksum="15CF8A19B3100FF8" modified="2022-10-12" version="1">MAQKFKERLLQGTGVSNKKFESDYAMKLMQKMGWNQGQGLGKNKDGQTDCVQIERRADQLALGAQQYSLGQSWNDLWWEQSYSSSLKNLKPISTKNLPQPISSDEEDKFSDYEKHKINRSQKPKTYAQSVKVLIEQDSDSENDTFIAIQKKKIKKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VKK2</accession>
    <name>A0A8S1VKK2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0670157</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000067">
        <property type="protein sequence ID" value="CAD8177367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VKK2"/>
    <dbReference type="OrthoDB" id="268027at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071203">
        <property type="term" value="C:WASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019309">
        <property type="entry name" value="WASHC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF263">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10152">
        <property type="entry name" value="CCDC53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8177367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="184" mass="21788" checksum="1887CCF9C8D4B666" modified="2022-10-12" version="1">MESFIDLTQLEPIPYKNTIAVLNEYMLQNIDFLNKFGHFNENKLFEMEVVLDEIESKVLLLEKKLESIPPEFFNGLVVPPLQQQQQVAQPQAVEPQNKEYEGEWKDSKMNGQGEFRWNDGRKYIGSYKNDKKDGYGEFNWPDGKYYKGQWKDGKQHGIALYKGKDMPNERQCYFEEGKRTKWIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VPF5</accession>
    <name>A0A8S1VPF5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0680239</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000068">
        <property type="protein sequence ID" value="CAD8178045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VPF5"/>
    <dbReference type="OrthoDB" id="4869960at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8178045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="230" mass="27287" checksum="4C34AC004CA7DF61" modified="2022-10-12" version="1">MKILLSQAVMIHLLNFGINKMDGCVNITLKIILIMSIHQVLNIIEYSQNDQKWNVIQKIKVEQHGRRLSFINDNQFTFQPYIQEQMHVYQINSRNQQFFKSKQITVKSGSLCCINQFSQQYSKCKCLLVNKNGYHINLIRKLENGDFVTEQTIQFDHLIFLVNQVMMANIQSLGIRNQKKYKLENARNYEQYLIYMRQLEKNIIKLINEVSSQIKLYKTKFSLRNLLKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VRY8</accession>
    <name>A0A8S1VRY8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0690214</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VRY8"/>
    <dbReference type="OrthoDB" id="297169at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000085">
        <property type="entry name" value="TBC domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000016">
        <property type="entry name" value="TBC1 domain family member 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF20">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="77"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="495"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="495"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="523"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8178472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="570" mass="68578" checksum="EA07C3A73A11BEE6" modified="2022-10-12" version="1">MIKEKDEEIKIMTNFLQSKLYIQNMKGDMQKNEKKRSLLNVQIKYFQLFDEEQELVKICNNSYSCVNQALRFVLREKVQSQSLKQYWRVLSNINYNLNSNTCYYQNIQNIQNFPSQFQEIIKKDIVRTTQDEECRVKLSNVLNAYSIRNAQVGYCQGFNYIAYYFLQYFNEEETFWFLCNLFETILPPNYYNHLYGVQCDDFIIKEILVFLKPQLINHMEKLQVETSLFTIQWLVCCFTFHNKLSETIIDLLLLDGSKALIKCVLTYLTFLEPILLQCEDIGTFIISIENFLQNYQDREQFIKEYNRIYINNKILQFVRQDYSRKVVTMIQQRSNQIAKQLDTYLLDLKQKKCNLESPVCQYILETWNRNKKHLDFIIIQEQNIKIIQDYEQTKYRKSILDNNDQMIFRQNHICKSDDMILIREIQYTDVQDVEFVNIKQIQKNNQVSRRARSPIKDILLFPLKAFNQKEQEQKLKMNPQKYKIEPYQIQRTVKSYQPQQSPNSYQHNRARSPQYRRSYQPKDMSNSQYVGSTQRTQNEEPPTIRESITIQFLTELSKNTGLINLEFQYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VWH8</accession>
    <name>A0A8S1VWH8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0750239</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000075">
        <property type="protein sequence ID" value="CAD8181171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VWH8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8181171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="62" mass="7397" checksum="004AAF0DDD5267CA" modified="2022-10-12" version="1">MQQISQFIHKFSQEGEEKLNLNNYKGRLSKILIGNEEFYFWILTSITGRVNCLFCQRIINIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VZZ7</accession>
    <name>A0A8S1VZZ7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0780022</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VZZ7"/>
    <dbReference type="OrthoDB" id="283378at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8182097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="123" mass="14190" checksum="9B2229F3AB7EA5EA" modified="2022-10-12" version="1">MIATEQFDCLPIEEVSSMLGSIQVKEYPIFDVQKGVLTKKKVKYHNYDDEEIEIKFECEQSQYCTANEKIVKIPPKGLDFLRIRILVPSNCQEHQISLTVDAHDNHTGLIKQKIIFKLNVVNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W2X2</accession>
    <name>A0A8S1W2X2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pru domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0800182</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W2X2"/>
    <dbReference type="OrthoDB" id="7777654at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061133">
        <property type="term" value="F:endopeptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044867">
        <property type="entry name" value="DEUBAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006773">
        <property type="entry name" value="Rpn13/ADRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044868">
        <property type="entry name" value="Rpn13/ADRM1_Pru"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032368">
        <property type="entry name" value="RPN13_DEUBAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008207">
        <property type="entry name" value="Sig_transdc_His_kin_Hpt_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12225">
        <property type="entry name" value="ADHESION REGULATING MOLECULE 1 110 KDA CELL MEMBRANE GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12225:SF0">
        <property type="entry name" value="PROTEASOMAL UBIQUITIN RECEPTOR ADRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04683">
        <property type="entry name" value="Rpn13_ADRM1_Pru"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16550">
        <property type="entry name" value="RPN13_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51916">
        <property type="entry name" value="DEUBAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50894">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51917">
        <property type="entry name" value="PRU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pru" evidence="6">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="DEUBAD" evidence="5">
        <location>
            <begin position="158"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="HPt" evidence="4">
        <location>
            <begin position="181"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphohistidine" evidence="3">
        <location>
            <position position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8183303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="264" mass="30966" checksum="5E799C9BFDCBBDAC" modified="2022-10-12" version="1">MFQEVTIPCGKYDFNPETKKVAIVKAKGLLTLCLNEENELQLKWYNLDQDSKLEIERFLIKGFTFFEKVKGQNRVYLLRFTDDDQKYFFWMQSEDSSLDENYCKQFNNVINAQVLDDPIEIEQQPPVQTQTQLHSQPVQQQMDQQQQQLLQLLQQQLAQKVGPGLSLTDLLSSEFLTQISNDQEYFDALKEYLPSDQQNLEQFRQNLLSPQFKQALDQLTHALKGRERSSVIQQLDLDYRVLEQEFDGVIAFVKAIIRKAQNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W399</accession>
    <name>A0A8S1W399_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0800108</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W399"/>
    <dbReference type="OrthoDB" id="293070at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8183157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="221" mass="26255" checksum="FDDA6A774B0A532E" modified="2022-10-12" version="1">MQKSLTPTSDIKYNCSQKIHLLKQKRNKDMLNQAIQDLKNPFDPLQVSKALKFLENQMQRKALLDQIIYIREQKRKKEEFDKMSSMRGILNKVSKRLSIVKNQEVKYTRDHNDFKIMQEERNKFDKLQYENLNIQVNEKRPQLRRLSSLSFLTKETDQIKFQVESNNDQKSMFARRQTQRNATLMVNAILKKEIPVSPGSSEKMIGNKIFKKLVSQDQIFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W3M1</accession>
    <name>A0A8S1W3M1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Atg6 BARA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0830141</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000083">
        <property type="protein sequence ID" value="CAD8184594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W3M1"/>
    <dbReference type="OrthoDB" id="20368at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034271">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043548">
        <property type="term" value="F:phosphatidylinositol 3-kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045324">
        <property type="term" value="P:late endosome to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007243">
        <property type="entry name" value="Atg6/Beclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040455">
        <property type="entry name" value="Atg6_BARA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12768">
        <property type="entry name" value="BECLIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12768:SF4">
        <property type="entry name" value="BECLIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04111">
        <property type="entry name" value="APG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Atg6 BARA" evidence="2">
        <location>
            <begin position="149"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="22"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8184594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="341" mass="40164" checksum="FA47BD995205ED83" modified="2022-10-12" version="1">MEEQEECFDCKIIIAKKELYCEKCLQQQMKKEEEEVKQLENEINELQIQLNQVRDEISKMEINSIEKKNDKIELDKKNLDKEIQQLELDEQKQKNELIKLKNQILEQEHNEDNLWEHINKFTRKLYSLFESNQIADNKLSQLNNELDRLSKLDVLNDLFKISVQDEVATINGLQIGKKGEQPVDWDDISAGVGHLTLLLVYLMKKFMYTYQKIESIELNGSFSKIKIKDEYQNALCTKTLNLYLNNKTSQKDEEEFSDAFLQLYLEFQQFCKYLQDGDILKKRKINLNLPFSMNGMSVDNKILSLKQPATQKWQEWTNSVKKFLANVKALIVANAQFDLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W5Q0</accession>
    <name>A0A8S1W5Q0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0810140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000081">
        <property type="protein sequence ID" value="CAD8183715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W5Q0"/>
    <dbReference type="OrthoDB" id="300990at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="136"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8183715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="262" mass="31480" checksum="CB73DD1A2F00CFD0" modified="2022-10-12" version="1">MYPQILRCKLKQKQTIFNNNEHSIEFLKKNLINRQISNHYSNSNQDQLVYQLFQLQQKVRNSEKLFTKKELYLQEQILKTQKNFEKTMNLMKFKYQMQIEDLYSELKIKDEEIYSLNLQLQQNNLKKIGKRLFSSQQQQKKQSINEQQNSDSSRKYCQFKMPTRSQNSSRKNDSNIYQKQLRRIRIQTSEFAQKVENEKNLIQSDIAILKDQKQIIKGYLKLNKVQNSDRSQYNSPIKSNNNEINKQSSSSFGVKEYFSQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W6P5</accession>
    <name>A0A8S1W6P5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0820155</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000082">
        <property type="protein sequence ID" value="CAD8184132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W6P5"/>
    <dbReference type="OrthoDB" id="303238at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="77"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="190"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="107"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="77"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8184132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="335" mass="39417" checksum="2C37B7C7ACA385AD" modified="2022-10-12" version="1">MNNLYCQKKKRIHEVTYAQKVINSVKPDIENIKLNENLFQAQQMARHSSTLIKDIQKDVRLTLKKYDSYVKMSESNRKITDKQVSESTRRYQSESPLKQQESTDKELETLRQQQRILMTLLNNLQQQENLIVSQSFPIPEPHPKSVIDKEWFQQKTKQLQQIQNTQAQIENTSSKRLDKSDVIQIDLSKVKRSPNSKSSSAQNFFSPNKNPIKNSLTYIQNSTSSYAKLHRKQSNSPQKWGQYGVINQQKPQKNLIVSKRNEQWKQRVDNKIRNELIKKQEREIKACTFKPKILSKTPKSNRNSKETSTDFNELMFLIDDINEFNDKNKRSTIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W9N0</accession>
    <name>A0A8S1W9N0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerase III subunit RPC3</fullName>
            <shortName evidence="4">RNA polymerase III subunit C3</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0840165</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="4">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the eukaryotic RPC3/POLR3C RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8185105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W9N0"/>
    <dbReference type="OrthoDB" id="272392at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055207">
        <property type="entry name" value="POLR3C_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039748">
        <property type="entry name" value="RPC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12949:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12949">
        <property type="entry name" value="RNA POLYMERASE III DNA DIRECTED -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22536">
        <property type="entry name" value="WHD_POLR3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase III subunit RPC3 winged-helix" evidence="5">
        <location>
            <begin position="272"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8185105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="382" mass="45081" checksum="32DD67CDE5C60BCC" modified="2022-10-12" version="1">MHTFRTLYFSQLAEQHFGKAMGKVIHVLDQYGPLQYTDILKYTQFDDLKFLEIVITLGIKHQIIGYKIKKQQDNASEIKQLFQSDKEKKQSDDQKQTKNKQKQKGLIFVNHNAILGREYVSELLNIEKNYNIIIDYALKNGVFGIKNLISENIKESALEVLVNELFDKSLLQIVQVDQQEEIQEATYNDTKNKSDKKTQKAKKKIEVSTQKQQSLTQNSLLQINWTGLMDIVKYNTIMQQLKQIYGISSFQDQNLYKQKQKELKENLEFKWIEDKYSQKHSRVFNLLKLKNCPLDDKQVCDLALLNYQDSNTILFELLTDQLIKTQIIKVNDKDKQFFQFDQKLVDEKLKLHFYKGLISCKDKKLYFGILNQMNLMQFDVRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WAV1</accession>
    <name>A0A8S1WAV1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2-phosphoglycerate kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0880184</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8187174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WAV1"/>
    <dbReference type="OrthoDB" id="271259at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33477:SF2">
        <property type="entry name" value="2-PHOSPHOGLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33477">
        <property type="entry name" value="P-LOOP NTPASE DOMAIN-CONTAINING PROTEIN LPA1 HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8187174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="374" mass="43815" checksum="0E27FB157200F8C6" modified="2022-10-12" version="1">MMSEVNERKILTITKNDNVLDLVKIKVRLDEHFYIFSRFMISRMLTLSRIKKLDAIQIAKDIKKQLIDRNSLEISSNELEAIIFQTMNKFGYQNNIQKYQMVSNFYRHRTPMIIIIFGAPSIGKSLLANNLAERLNISNVLQTDIVEMVMRSINPEQFNYDQNEDFITKFKKNCRLIRRGVSTDISKCLSEGKAVIIEGSAAMPEYYLEAIDKQDPQNDEQQLQLITSFTQNDIKLKKPQRKDLLLFNQQIKLNTQFKIIFPQPDQDLEDDQQIQKHLKLCKDLNKIDQSHSMILGFLPILSRNDHLYNIENNTSKKIPKDLIEEKLNQYQSIQNELLKQRSKCILLPINLHNLEETLDTMHDIILQKILDQST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WEY2</accession>
    <name>A0A8S1WEY2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0880177</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8187160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WEY2"/>
    <dbReference type="OrthoDB" id="310129at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8187160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="218" mass="26366" checksum="5DC626D32E9DCDA3" modified="2022-10-12" version="1">MDIYHLRLENYQPKQSFYLMNKILKLLIALQMLLVFFRIFVRNQEPFFLSDCLNACMMMLTYCQKNYCLALITMFYIFSLIYLNAIFFLQFVEYKLPFFIDYTKNFTLMLIFSTLLYIFESIMLYQVYKEFKGIIYDINQEYLENVQQEQQQHLEIEMLSQIQEQSVQQINSNQQQLAEQEIENQSIQEPNIAAGSLQRRMNNQGSRTQQELQQQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WID9</accession>
    <name>A0A8S1WID9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LITAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0940215</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDIP1/LITAF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WID9"/>
    <dbReference type="OrthoDB" id="435182at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006629">
        <property type="entry name" value="LITAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037519">
        <property type="entry name" value="LITAF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292:SF6">
        <property type="entry name" value="FI16602P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292">
        <property type="entry name" value="LIPOPOLYSACCHARIDE-INDUCED TUMOR NECROSIS FACTOR-ALPHA FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10601">
        <property type="entry name" value="zf-LITAF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00714">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51837">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LITAF" evidence="6">
        <location>
            <begin position="73"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8189798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="160" mass="18142" checksum="13E9DE7A765AAF62" modified="2022-10-12" version="1">MQNNQHVRLTQEITPTPYEIPTSQVVYPQLPNPNMQIGQPIQPIIPTQPFVRQIPLQQQQFPQFQQQQLVTYNPTAISQVQHVYSKYPHMITCAQCQRQVQTQVNYEVGNGAYAIGALLAAVGLWLGCCLIPCFVQDCKDAVHFCPACQAKIGKKRFLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WJH9</accession>
    <name>A0A8S1WJH9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0930024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000093">
        <property type="protein sequence ID" value="CAD8189082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WJH9"/>
    <dbReference type="OrthoDB" id="291784at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="15"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8189082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="525" mass="61872" checksum="FF63C4D35F7185ED" modified="2022-10-12" version="1">MGQVQQSKQHFLKNWQKLDQHQSKIFGSLIEVYQNQQEQVAVISKLYQNDDQYQIELQKLQQYQQLSNIPGLIKLLEINKQEEQYLCSNFYKIDAIFEYGIPFTSQLPYYQFIQQVMHTLIELQNRDKYHGDLIPSSFLFKNQVKLFLPNINHYSRLLNGSQEDCYLSPELLEYLGRGSYRPEYNKEKSEIFSIGLITLELILQESIQKIYNFEIYQININILDDLLQKADNKLISKMLELQPENRPNYLSIYEESLVEMMQQSAIKIENQQSNYLNEISNQQIKEILPIHQSKNLHIKTQIIQSTNTLKKTNPTQRSVQIISEHKTKYLKSQLKQTQKKQVKKVNPSISEQNNHDQIKIHSKQKSNNFANLELLQFQDNNIYQNSNLISPQNQQFYPNSLQYLTPSQSPNNRYSYKKSHQSSAIKQQSLEPNEYAFLHEQIHVPYNPGSNFLDSSRQQELYEVVSPKSQRPTSTLKDISNKKLQINQTKTNQNIKESVQKKPQLLRIQRTNSISPNFQKNLTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WMG3</accession>
    <name>A0A8S1WMG3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0980154</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000098">
        <property type="protein sequence ID" value="CAD8191244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WMG3"/>
    <dbReference type="OrthoDB" id="5351233at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19756">
        <property type="entry name" value="Bbox2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047153">
        <property type="entry name" value="TRIM45/56/19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462">
        <property type="entry name" value="BONUS, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462:SF296">
        <property type="entry name" value="MEIOTIC P26, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="6">
        <location>
            <begin position="148"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="75"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="199"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="75"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="103"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8191244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="356" mass="41065" checksum="1960325A8702501F" modified="2022-10-12" version="1">MMNVSKCSKCSNVPDDILMLTCSHDLCLLCAAKSFSQQPPKRSKKFFVCDICSSNTELDANSVYELEKLHLTNALQDRSNRKPASQQKPNISSEKQLKPRSQNRQDNKENPKEKPIKYTAQTIEARVSIHQSQRHMSKQQSENSFIQHSRGMCVDHPDEEVSYFCFDCNSKCICPECIIHGIHKNHEVKTIKKSYPIVRKQLEEQLEQNNQCIIQVENQKQEIEKKQIQQQAIQDHLRLQIAQEIQALHQLLNSKQSELLEKVDNLPTIMEQQDGYINRLNEVEFKLQNFNEKINDIIDSKDECGLLNYYGSIYGQPTPQIPPLPNIKEVQLSNQIFSQINDVKKLIGGLDIDCNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WNH0</accession>
    <name>A0A8S1WNH0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0950180</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000095">
        <property type="protein sequence ID" value="CAD8190171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="536211at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035737444" evidence="5">
        <location>
            <begin position="18"/>
            <end position="1289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1008"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1053"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1114"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1146"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1168"/>
            <end position="1189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1201"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1239"/>
            <end position="1261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8190171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1289" mass="149863" checksum="8D3AEE8312770DC8" modified="2022-10-12" version="1">MLFKFLFFQCLGIVVEAQYIQNFVTSQITNNEGWAIINPYNGATTHITDCGGNIIFGGYQVFGCISSRDTQMMKYFILPPHYHLRINVMHWKIDDWPASQNFYIILDSKVHYWQVTANTGSNLCGDIGTEQSSLICKEYPLHTFNSVTVSFHSSCNTDNFWGITDFSIELDECYNGCLFCLDQTTDCIFWIVWKSYFDLQNLDNGSEGWKINQFSRFQTTNNQFQIKFLVINKGLSAINYFLLPDHNSFIIQFCVDYPNYAQSKFIIYLNDEQLLEFTNNVKYVPYRSSIINDSRNQIKLEIQSINDRIAIRELKIILRGPINLISCIDDNMEPFDGCFAKQESCQQGCLFCVNGQCLMCDNKWIYDELNNNCEPLCGDKFIIVNEQCDDGNNIEFDGCYQCQFSCPLNCYNCLFGKCQECITGYYYSNGICNQINEYLFQGTDLILRKNLNEYQISLAFGDLKFNQLHLYYQEKLIFQNGIETFESLCSPLCQVCQYNKCLKCLGNHLLLKNQCIPRITQGVFLIEDGIHLSEYLIGCNQCQESCQIQCLQCQNSYCILCIDGWQLLNGQCKQVCGDNQVAILSQEQCDSNLEDCLNCNIYCSVNCQYCLNNKECLICNENYVEINHKCQLACSKDCKECIQGKCVDDILNGEINFNDTQDQICGDGIKQQSELCDDGNNIQFDGCYNCQYSCPLNCVNCIDGICYECEQQLQLKYNQCYEYCGNGKKNQDELCDDGNIIDADGCSSSCKIEVDYKCIENDNGLSECQYYESPQMKLKFLNQTFNKYYIDISFTQPIYFKNNYQLESLFDISIDDIKTEYYAIKLESNYQPTIDTVLNFQFLLEISLFISTNNSFAQSNIQLTNEAFNENEMKLLNPSLKMLLKQYIQMNKADIDKTKKLSEYQEVMIIGQGIICVISIMSGNYQMLIEILDNLQYLAYLKYINIIYPENLYLFFESTNLISINPVLDFFSVGEIFEKYIYQDFIQSYGKLKFYSLNANLITNLQFFFIQVIAMIVLAISLNTICYISIKLFEKNITRCFLFINANSWRYQFLFKVINFIYNIFHQILILVNQFNQNGVQNILLANAYDLLFKTFLFLTSNSRNNKISLIQSALSYFFLAALALTIILLLKHSFKKIKQSMNDRYKAFYLLKQFFFCYYLICFQNDQFIQLLMLTTTNLIYLLALISVDLNLSTLVKMQIIIQEISIIVFTFTSIFHLQDYSFLISGQNQIALGFSQIYLLISGLLWIFIKYVIMIYSKIKQKCIQYKKKKQNKKFRKVTHLIFQMVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WUD8</accession>
    <name>A0A8S1WUD8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1030015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000103">
        <property type="protein sequence ID" value="CAD8192922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="307276at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="217"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="262"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="308"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8192922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="487" mass="57684" checksum="5F806DA2F16A4DEF" modified="2022-10-12" version="1">MCQQNDFSIXIGFKKIVQKIEDNKQQQLSILENLIISNIKQVESFQIEINNLKSMIIQQLDQIINNTKDWILNLKQIGFKYSEYSFFKELDLIIINQQNNQNDQINCQNQIKILNQSQITKINSKLEQFKSFQEYQQLKNILNKLNEQTQNIFIIQQQQKQQLSPNQLLFIDNTIEQKEICNAIVFDSTGKIMVSTCENKILIWNFNNGKIQKVQTLSEHQKDVICLIYSKIKNYFISGSNDGSIILWKQLNNNQWQSSKPQCEHKKQINCIILTQNENQLISGSLDSLINVWRIDFINNQLTFLYSLQKHTNSVTSQSLNESERVLVSCGDDKLIIIWQKDANNKWTFQQVVKQSIQDVGTHVKFIKEDQFILLPYKFNCLYVFQQKDGQFQEIIEKKVQFKKINYGYLNQSFFPIIFIKDKNLICLRHKDYIILLKEQNDGQLQIVQELDCQHWNIFGTVTNNGQYLIYWGENKMKYEIYEIQYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WUL2</accession>
    <name>A0A8S1WUL2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1010173</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000101">
        <property type="protein sequence ID" value="CAD8192467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WUL2"/>
    <dbReference type="OrthoDB" id="322722at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8192467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="276" mass="33279" checksum="96414CA2D4918719" modified="2022-10-12" version="1">MYKSQQKRHLQESINKVNKESEAEIQVDYDEFLEVTHKDLQNLEIIDYYQIAFEVWNILEENLIPQQSQISQESVGVHKQLENIFETNLQLPNKIFKKITIQQEEIILFANDALKEYDLTIYEFYCEFINYYQMKQIEKQKNFGKSIDTDLFMHDLLEYSIRFAKIMSKNQMTQVQYKQQGFLYQNERTEDQSLSEFFNYDDQNGAYKKVIRSCSLVQQKGANFNLRILQQKCWQKKRIKIRIAQSFSKFSSKTMPRIIIKLEIHLFCKKKIIRCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WW26</accession>
    <name>A0A8S1WW26_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1060097</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WW26"/>
    <dbReference type="OrthoDB" id="290857at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8194244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="152" mass="18122" checksum="F8BAD1C77E482C81" modified="2022-10-12" version="1">MNLEQFISTEDDWAHQSYHENDDFEFSCSPIMFGRPSNKNKNDFELDKEDYFIDAPKLEEQTDEPDFNQLLNTPSIPIKKRQIRKNRKVKNNTITNLYKRMFFEIINDSQSQAQIQKQLEHCQRMKSVIDGLEKTLINVKTLLVHKLKQRSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WXD0</accession>
    <name>A0A8S1WXD0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1070019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000107">
        <property type="protein sequence ID" value="CAD8194503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WXD0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8194503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="130" mass="15586" checksum="1360776C76C7F4FE" modified="2022-10-12" version="1">MIVENNNLIQKKSTQSTQSSQINQISNEEREQLLLIAEEFEKEQEQNLQRNLKLRLEEKQMEQFQYKDAFFNYNHSDIDDYLKLQEEYIGPWQVNFERVQHGQDFGVKVPSMTSLVKGLMDQWEVEQKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WZH1</accession>
    <name>A0A8S1WZH1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1050114</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000105">
        <property type="protein sequence ID" value="CAD8193930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WZH1"/>
    <dbReference type="OrthoDB" id="1452at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.570:FF:000005">
        <property type="entry name" value="26S proteasome regulatory subunit N7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019585">
        <property type="entry name" value="Rpn7/CSN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045135">
        <property type="entry name" value="Rpn7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049549">
        <property type="entry name" value="RPN7_PSMD6_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14145:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14145">
        <property type="entry name" value="26S PROTESOME SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10602">
        <property type="entry name" value="RPN7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21154">
        <property type="entry name" value="RPN7_PSMD6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="3">
        <location>
            <begin position="123"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="4"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8193930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="319" mass="37505" checksum="2D7BF7A3B68794D3" modified="2022-10-12" version="1">MEQEKTFEEQLEAVENKIKDSQENLGDVEVRDAMLEKASLQQKYNKYEDAIKTYIDTLPKSIGIGKKMDVHFLILQIYLKQRNLEKFKEHLNQQQILLDQGGDWERKNRLKVYEGIYCLMIRDITKACKLFLDSVATFNSSEIISYNEIVSYTVLTSMICLDRQSLKKKVHQNPEVVGVLRENQVLKSFLESFLQCDYKTLFQKFAIVNENLSHDQYLSIHRKYLIREYRVVFYSQFLESYKTVTLNNMAKAFGVTVQFIDRELSELISSRRINCKIDKVAGIIESSRADDRNQLYNNLIKQGDYLLNRVQKLSRLTDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X3Y7</accession>
    <name>A0A8S1X3Y7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1090043</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000109">
        <property type="protein sequence ID" value="CAD8195305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X3Y7"/>
    <dbReference type="OrthoDB" id="312687at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="22"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="284"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="483"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="529"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="567"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="671"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="140"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="285"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="567"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="598"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="611"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="685"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="699"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="711"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="721"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="778"/>
            <end position="787"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8195305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="797" mass="95734" checksum="1F2F7724B7FD6480" modified="2022-10-12" version="1">MKHNKQQSHYILDFCDIEDISDNESESSKQSNQQQQIIQSTPTPNRIELQIPNLQVKKETVEIPSILIQEEEEPIIRRKMDIQKQQWQVNFDELSEQEQQDSDRSQVAIEIPRDPSPPTIAVEEPQYIEQYFEDQNVIEEIDIEQEQLKMEQRKEQLKQDLITKKQQQLKQIPIVQQLQKEQQKVQEVNQLQNKNCEDKTKQIYKQKFYNPKNSLIYLRLKGREDLYKKQEYIIDEQTKPPDQDELLEGLECINNLLIGLSKIKDKKVGQVDKQQKILPLQIQKHRLDEKGTHQKESKIQKKDEKEKEIEQQQKNQKDIQKDQQQTELSIKQQQLQEAQLARQIRHEKQQQEKEKQKLLEQRQVYKILTKKQVNLGLVLYKCQLRQKQKDSIKWLRYSEIALISKGESEIFNFEKRISDQLFMQLHKANMRSVVITKQAIFDVVTIHYVEEQKEVEEVKQKEKVQVCKEQKEKENPIVEEKQIKEKKVTQNNDKKKKNQEQQRNQVQQNVDTLQQQDILQQQDIQNQQQQETKRIKNKVSYLESDEEKKDEDLLDADSIQIYKITQDRQKKLDKKEKEQKQSQINQQQVEESKQIESNDINQKQNNNNQIKEQKKVKNLKIKECPLSRLETNDEIKKKEQINQKRKEKRRQEYQQYLNEIEQEQLIEVTYAKKQKHQRDTKKQETNQQLIKTNKDQITNNQQENQNLEDQNNGKDKEETKNSNSTKRTGRQNNRNPKSPSPQNESIEPGYVSSKRIYENRDQKKKRLRVIEDEESEFSEQNAKQNQTKEQKKRVRQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X858</accession>
    <name>A0A8S1X858_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1110023</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000111">
        <property type="protein sequence ID" value="CAD8195906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X858"/>
    <dbReference type="OrthoDB" id="299368at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="51"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="630"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="224"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="396"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="77"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="111"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="164"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="184"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="641"/>
            <end position="660"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8195906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="680" mass="80233" checksum="4431C8C95A84392A" modified="2022-10-12" version="1">MSKNQEKLKKELLEQVSRDVQNAQDPQKEAFKLVQNANETFKQFESALKKPLDDEMLKQRGSSKKNVQFADTIKSSSEMDRSKSVENKKEEKPILQPQIKQNEIKAIVPDRQSRSTKKEGKVEKSTQKINTKQDKPTTAIPEKKPQQPQQKENQDSKKSNQLDQSQKQQSTLPQQQNQDRERINQYQKQVLSESESSSGQDDIKREKGNHSHSCRKHSKEREIANRYEEMIVKNQESAQQLQMQLNEQNIEKTKLITENTKLSSINDMLQQEIRNLNKQLSQSKMQIEQLDKQLYDLKQASRSELLIVKDQLQESKFQCAELENRNNYLNKAKDTFEQNLKQFESEVGRLQKINLQQTQVAAQDKLAILEKDGTIERLQKLNHQLSVDLNQINTLYKEVNEKHKSLLAAYNSLENQKITIENDLEFRKKDQQEHLSSLKEKIQKLTNQKSIYDDELSKMQQATQDAKAERDQYKLQYEQMKVLCSESEELRKRLKLREVENEQLQCENDKLKWKVRKDPNGILSDLKIMKLQAEDPNAKVSMEQLSKLDNQVRQLQEENNRLTAENKQLLKQIQQTELYVQEVKSMFDRERSFLEEKVFHKEKEINEMIIRHRHEMAEIENKYHDLQRRQENDNKTRQTHQNYQQQSYSQYNQNSVAQQSGSKVSPYSVPQLQAKIKSYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X943</accession>
    <name>A0A8S1X943_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Arrestin C-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1150113</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000115">
        <property type="protein sequence ID" value="CAD8197452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X943"/>
    <dbReference type="OrthoDB" id="284153at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF17">
        <property type="entry name" value="FI21816P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="1">
        <location>
            <begin position="211"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8197452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="380" mass="43965" checksum="12641B0D8DC1973C" modified="2022-10-12" version="1">MNRNVLFKFFDPVTLAGDPIRGECYIRITEEIPKAQIVLTFQTKMYSKIIEKKQIPYDQSNPQMIPEKLVEKAQRIQVTLDSPFRQPKQTQKKYELNGDIMYRVIRHYGTHESFVYTQELYTGQVKTGDYKFQFSIPTQYNMSSSFQYKNEDGLKQAKCGYKVTLRVDLPDEASTLMMESADVFINGRVSSECEQFRQCEGNIVQYLCLNRGTVELSLKLNKNNFIPGEQIQIEYTLDNTRSQRSISRVEARLINKLTFIDDDEIERIIENVKVFQQNLGGVNSGQKLERQNASLEIPKNLRATIKTNVIRNQYFLQMEAIADAFLTWLSVPVICQIPINIQESQLPQKINLEGWNFLPIMNVSVNAFSNLTVQQSFVPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XG85</accession>
    <name>A0A8S1XG85_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1230130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000123">
        <property type="protein sequence ID" value="CAD8200117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XG85"/>
    <dbReference type="OrthoDB" id="298162at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="27"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="118"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="294"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="422"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="474"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8200117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="521" mass="62035" checksum="EDA01CE916BE94E3" modified="2022-10-12" version="1">MNIRQPLQSYNSVERNPEKSCKSISEIETFLKEIDRLQGENQKLKDRLKQQPSISMTMENQKVLTENQDLKNQLELLQHKNEVQEKTHADALRQLDINWKQDVATLKFQLEQQEINHRQSSRQEIMQYKSEIAKLQQNLSKISNVENNNQNLVQVLENELIQVKQINNGLEEKLDQAISQFDLQSKDQEFTLMKQQTIINSQEQYLNELKSKLQIQKDQNLDLLSQVKKLKEVADNKTTQASSLSSRVKELENDFKHVSSTYQEHKAQLQQRVEELILSTGKQSEQICLLQTQQQRDQELIKRLQVNLENEQKNREMEVREIQESKAKLIQFLQQELLQQKEQNHQLRSDNQMLDKQLNELKQYYQYNFRDLECKTNMLEGECNRLNNIIAQKDQELVIIEMQMKSTNNCQEREDEKIHEVIQIQEQEINLLKQQNESLINDLSRQADNYRHLALQHKQYQKFAEDVKLNNITINELQSKCELQDKEIERLRMKILEKSQQLEKVQKDNLDYQAKLKTRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XH35</accession>
    <name>A0A8S1XH35_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1230040</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000123">
        <property type="protein sequence ID" value="CAD8199972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XH35"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF140">
        <property type="entry name" value="PLC-LIKE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="157"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="63"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8199972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="495" mass="57678" checksum="21B41E2D07307B84" modified="2022-10-12" version="1">MRKKPFQDNGIYVEKHAKALLDYQQKQDEKDINTQSKYRIKAIKYKNNFLESQEVIKALKMENQESKKIISELLLDIEEMKEREKLLESELQEQKQLTQSAIEQLEQELQESVEDRLLQEQKQFQEKLTIQSNDAIEKLKKHFDEQLQIIAQTPKLNASPAKKKKKKHTFTSTTNLRPQSQTTGGNTHLKSSLHLHDIVYLGAHNSPMASCYGWHYTQQNVTITEQFEKYGARHFKIPLHWHVQNGVPQIVIAHEENGKSNCKMSLAQRLASKPEKAVDRLKELVNLTFKYPDEVIIMKLESKLLERSKDNGTLGWSDIEVAQKLHNLLVDIKAKQRVIRFQENNVPTLGWCRDNQKNILITIEPREQILGDLQEYTHDSYIVSAQVDWQTDPSKDVTTGNVARGLVNKNNGYIQSPFLEIHLNPENSLKLDMQYRKLYNQYDHVKKRFLQYYKHCGKMPNFVVADFVDQGDLSKIIDEINTNINQNDGFKPELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XIN7</accession>
    <name>A0A8S1XIN7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1270057</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000127">
        <property type="protein sequence ID" value="CAD8201270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XIN7"/>
    <dbReference type="OrthoDB" id="2310150at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005399">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150">
        <property type="entry name" value="HYPERKINETIC, ISOFORM M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150:SF2">
        <property type="entry name" value="HYPERKINETIC, ISOFORM M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="3">
        <location>
            <begin position="16"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8201270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="349" mass="39756" checksum="F424ACA334D8AF62" modified="2022-10-12" version="1">MQYNRLGNTGLLVSQLGFGNMVNFQPEDEEVNVAIIKRAYEAGINFFDTAEFYQHGEAEKQLGRSIKILNIPREKLVITTKVFGNQAPGGLNVNRMCTLNRKHIIEAVNTSLKNLQLDYVDVVYAHQYDCETPIEEIVRGFNTVIEDGKAFYWATSNWNAAQIQEAINYADSHGLIRPIADQGEYHMLERKRFEQEFVGLYEKYNYGTTIYSPLCGGFLTGKYLEGIPENSRCGKDNGWLTKERATNRFLLKYTSNPKCVEGLKQFVALAKELSVTPAQLALAWTMRNKDIDVAITGARTVAQLEESLGSIELLKKFDKDLDEKLEKVFENAPKQEMDFRTLKPIPNRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XJ24</accession>
    <name>A0A8S1XJ24_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein translocase subunit SecA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T1260175</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000126">
        <property type="protein sequence ID" value="CAD8201155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="419440at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017038">
        <property type="term" value="P:protein import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002701">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1440.10:FF:000008">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000185">
        <property type="entry name" value="SecA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011115">
        <property type="entry name" value="SecA_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014018">
        <property type="entry name" value="SecA_motor_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011130">
        <property type="entry name" value="SecA_preprotein_X-link_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044722">
        <property type="entry name" value="SecA_SF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30612:SF0">
        <property type="entry name" value="CHLOROPLAST PROTEIN-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30612">
        <property type="entry name" value="SECA INNER MEMBRANE COMPONENT OF SEC PROTEIN SECRETION SYSTEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21090">
        <property type="entry name" value="P-loop_SecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07517">
        <property type="entry name" value="SecA_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01043">
        <property type="entry name" value="SecA_PP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00957">
        <property type="entry name" value="SecA_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51196">
        <property type="entry name" value="SECA_MOTOR_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SecA family profile" evidence="10">
        <location>
            <begin position="155"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="258"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="608"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1444"/>
            <end position="1515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8201155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1625" mass="189329" checksum="9E779AD0F4952BC2" modified="2022-10-12" version="1">MQVLIENKNWSIEENENFQSILCLLDLTIDQQQILHNQIQKFKSPEKIINFLLNADPDWDSLQKWIEKENSNQVQEKQQQSIIQEEKQWNQQQLMILKNQLSSQINSNEELNKILQYIQQTNLNKEIVANVLNCEFETFEEFIQLTQIFEGESKIVTRRPPQQNNDKQQSKNILYDNQQIKQLFTEFQSLNGRNNLQIQNLLIQLYSSFQYQQFDPTLIDFQPSPDLEYEDLFILCTKVKQYFSYYPRPVQLLSVIELYNHNNDQGRLAQIYTGEGKTLIVAMLAILLCKKKQINVDIVTSSPVLAIRDVQELTPFYKLFSISVAHNINGSQNQLKKMFPCYNSQVIYGDPHSFQADILRHEYSELGTMGDRKQGYVIVDEVDSMLIDGHSNKTLLSTPIPGMLDLTKVLRLIWDEICKQQPNISTDKKVMVIDQNNFYSVDLEEYIQKTLDIQLQDALYNFIPKFRLNYIDFMKKTWIENAILAMFHYHERKHYLIDNNNVRIIDYQNTGVVHQDNMQWQKGLHQFIQLKHNLPITPLRISTNYLSNVGFFKRYQNQLLGLTGTLGSQVTQNLLSKQYKLDFVLMPPFKQRLLKIEPGIATLSKDEWYQEILKSVQQQVNKGRAVLIINQTIQDVQKIEAYLKKYKIKSTTYVDDQQELKKVIGPQTVIIATNLAGRGTDLTTNEELERNGGLHVIMSFLPRNIRIQLQGFGRTGRQGKLGTAELIVTFPDNLYVGQLTQIKTIQDAINYYQTKQKQQMQNYFEVLVYFRDLNEQYYSDEIELEMEKLLNEDRCFNKFCEIAKKKVNMKENRAAFQSLEEKWGLYLEQYQDIGLNEEDIENWLNSDEGQNPKYLISQGLQKDDLKIFQKAAEIAKTDPQVQYYKGLSEIQSGQYKNGCESLQKAKSLFQIKIDDEKGFATASKLNRIQVEQFQDNQSLIEFQESVIMVPNENLELPDLDLTRDSESHNISAIQFVKQNDVQKDTVERKINNHLKVYNKVINNIDTILNTMSTFNPEEEVLDLSWMPVIMKDEKENVDLEQSIKDQQEVIDDGPLPKLGKIAKKKKEKSWLQYVAMFAIGLCQFVVGCAICAFTGGAAMPIGRALITEGISDMLTAVISAWKGIDIDWGNWGQQKMINIGSALVMAGPAGIKEALHLGGNAMKSLKKIGIAEFLKQIPEITAEGLKKNGFWLTDLDKEVVQNQYNTLKQISSTVSKVGNSSQLLNLAYDVLQNQVSNNVIDKEAATGIFDLINQCFQQSSGVEDFKNNFCQFAKKYIRLQMAQSKQGKSDSQIKEEVTQFSFIEGNKKYQQVKQDIEQYKQYKKAMENELKYFSTIMLDCFSRDQKIQKIVILLDLYYEKLCDFEKIKESITDKLIQSHLSQNFSFQDQTLQNACIQLQISNPQSLNQFYQKVIRPNIYNLCCLLSNQIKYKNPLEVILQKEYQKQIYAKRDQLKIDYENLNNQQQYLRQQQDLINPNQRTQNQIEQLNQMIDDFNNKRDQYQKKQNQLQEIEKLQYHELIINSIAYLKNKGMINDDDLTFKPQFLKQYQKYLENEKFDQAVQEILLSAIKNIPNEINNFQLTSKKIFSNKLKQAIEVAMLDEIKKYIFDTKMQKEDEKITSKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XL53</accession>
    <name>A0A8S1XL53_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1290087</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000129">
        <property type="protein sequence ID" value="CAD8201905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XL53"/>
    <dbReference type="OrthoDB" id="302794at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="24"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="70"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8201905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="210" mass="24751" checksum="17E5985896203270" modified="2022-10-12" version="1">MSDQRPYLLSQNKSVHHFQNTFFKQEPSPSKSHRKQLEQHGSPNHLSTSLSFSKQLANKPKQAQQDTSYVYYLIQQIKQMEQTIEELKQENQLLKSQQKIGYLDLQFQHKQMNKYHNILKSVQQVKLDNIKLRKSIEQMNLMMKIHLAPVNEMFQQLIHPESTAIAFAHKDSINHQQNDSINDSTLNIKIHANINKYQKCKTETQLKDQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XNA0</accession>
    <name>A0A8S1XNA0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1320101</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000132">
        <property type="protein sequence ID" value="CAD8202896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XNA0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8202896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="263" mass="32669" checksum="54C1F4951749198C" modified="2022-10-12" version="1">MYSNNQNYRQIIIERFNKSIYLYSKQYEIILQIKQLKMQNQTRLISVNLTKVKVENQKSRFCYLTLFIKQYYFNSVYYLRDLKKTFLNKKSQSSAFFKEHINQKHYLITSRIQNQKKNICKRNSLIFNQIQVRDVKFYCFNQHFRIIIYYQNESLIYYIENDYFRQIFNIIVINLIRVPFQNKLISKLSTLGILQEKLQKKLCYDFNCFLRRLWQQSMQINLSLQFWIIIINYHYKRMNFSWIQQYIKTFKVVKQGFEECFFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XQ28</accession>
    <name>A0A8S1XQ28_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T1320050</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000132">
        <property type="protein sequence ID" value="CAD8202798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XQ28"/>
    <dbReference type="OrthoDB" id="339325at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001337">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="131"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="21"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="254"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="258"/>
            <end position="259"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="8">
        <location>
            <position position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8202798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="381" mass="44225" checksum="C39A112B80D407A0" modified="2022-10-12" version="1">MNIRKPPREGLRSIENKENLPDNSSKPPPSRLTNYRLNNLTTYFKIRTPSTGRELAKFEDNFNIKNEQRYLSTKESQQCNTITNNTNQQQLRLSSKIQRKRIQGNLSAKDSTNHQESLYKQIPSTCDDKIYQIGPIIGKGSYAIVKIGTDRHGNKVAIKMYDKSLLKGERFNNLIKEINTLRILSHDQITKLYDVYHYTTYINLVMEYCGTESLYQLLKSTSTRTIPKEQALYIIHQLLKILHYIHDRNVCHRDIKLENILIQNKRVKLIDFGFSTFTNQPITCHCGTPSYMAPEVVSKLKYDGRAADIWATGVLLVTLLQGSFPFKGENERELFKKIRNNESNITNQDRDLQKLLSRIFVIDPYQRATAKELLQLDIFKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XRC3</accession>
    <name>A0A8S1XRC3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tetratricopeptide repeat protein 21A/21B N-terminal ARM repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1340100</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000134">
        <property type="protein sequence ID" value="CAD8203640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="312680at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056833">
        <property type="entry name" value="ARM_TT21_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25062">
        <property type="entry name" value="ARM_TT21_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="37"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="11"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="45"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="181"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="249"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Tetratricopeptide repeat protein 21A/21B N-terminal ARM repeat" evidence="4">
        <location>
            <begin position="251"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="283"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="317"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="385"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="453"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="487"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="657"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="725"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="793"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="834"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="877"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="911"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="945"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="1021"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="1055"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="1123"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="1191"/>
            <end position="1224"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="1259"/>
            <end position="1292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8203640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1383" mass="160168" checksum="BDC4037CB3AB8DA6" modified="2022-10-12" version="1">MKQQTISGNDVNESYTQGLNLMKQKKYQEAIQIFEKVLRIDSNHFQSLYEKGECLKLLEKYSAAIDCFQKALKIEPKNVDLILKLSLCLMKMEKYSDAQYNLDIALRYDSNNIDLVFNKSLCLKMMQRFDTAFYYLDKAIQIDKKNTSLLLNKSLWLQELKKYDEACQPLDQALQYEPKNIEILLNKSICLRMMKKYAQALELLDKALQLEPENVNLILNKCICLLAFNKYDQASTVLDKALQQEPQNFDILLQKGQCLKILNKFDDALQYLDRALKIQPKNLEVLLNKGICLQMNKKYDQASQVFDKALEIDNQNVELLQNKGNCLQQLKKYSEALKYLNKAINFEPKNVEILINKSECLMMMKDYQQACQSITLAQKIDSKNIKVLLIKSKCLRKLNELKEALEYLDLVLSIEPKNFEAQINKCLCLKLQRQYPQALQQIEKALIIEPKNFDLLKDKGICLTYMEKYQEALQQFQQVEKIQPDNVEILLNKGICLLQINQYDQALIQFNHALKFDSKNIDLLINNSICLYKKKEYSLALQCVEKGFKIEPKNITILKIKSDCLIELKEYNRACDSLDLLLAIEPKNIEILLKKFNALKIIEKYDETIKILDRILSIDNQHQFCILQKGFCLKLNNNYEMAIQWFDKALRFDSRNLDVIDAKGQCLFMLNKFDEAIQCMDLALQINPKHVQSLAIKGECLISKKLYDKALSILESALQIDANDVCTLSLKGKCLLMMQNYQQAIQYFDKALKFDKNHFNTLQLKSKCLLLIEQFDNAIIYADLALKIKPQNFDCLFIKGECLKRIGKYQEAIKYLQIAISIQPNHFLCIYFKGIIKNQQGECLQQIGSYQEAIRSFQQALQINQNHIQCLRNLGFKIFLILLGQCLFQQGQYQEAFKQLEKALRLDPNDFNSLYLQGQCLKHLEQCSLAIQYFEKALILKPLDINCLNSIGGCLMVMEQYDQAIEIINKVLNLKPNSVDALIMKGEYLQIKCNYKEAIVNYKAAVQINGKIYNALFKLGSKGFIVLGICLKLIKNYSEAIIYLEKSIQIDPKNLYSLYEKSECLRLLGQYSEAVQCLQRVFDINPNHIESLISKCDCLKGLKEFDIAVRLISKVIKRIPFNINLLFLRAECLKQLGRYENAIQDLNKILTVDPLHLLSIILKGGCLSLMGQYQESIFWLDKALSLDQNNVEALATKGMSLTMMGRYTEAIEWLDKVLAIDPQHLYSLSCKGGCLVNMNLPLEAIIYFNLALQVDQHHLYSLSSKSMCLIMMGQYHQAIEWLNKVLNIDPNHFYSLSSLGYNIIFIQRRMLINVELILRSQYVVQQSIVYKLFRCVLLILKSLLSIYDRSILIGYQIIQQSSIYQQQTCLFVVCKGSLFKSYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XRF1</accession>
    <name>A0A8S1XRF1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1340115</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000134">
        <property type="protein sequence ID" value="CAD8203670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XRF1"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="378"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8203670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="513" mass="60835" checksum="FA6CBA3A2DA5CECF" modified="2022-10-12" version="1">MSNEDQSIEGSFASVYKGHMFDNKNELIAIKIIDKKKFQNKKNRKNFIQDYINREKVNQQQLQSPNVVKMIDVIEDDDNCCFILEFCEGGNLQKKLEKGALPQEEALQIFKQVVNGYKELRDKNIIHRDLKPENILFSKKIAKIGDFGFSKFLDDLDQTILQSGVGTPYYAAPEIRTGHFSSKADIWSLGLIFYYMIYGSLPVEILKQVNYKEQKIDVLECPENKKIPGDIIDLLKKMLVVNPDKRMFWDELFAENLLHLIIPQNNEILDQISEQKAIIMKQTLQNENKVENIYQYFIYILDILQFIRQIIIDVQILRDAVMFSREQQLSFLIITIKYMVNELKFYQGILKGQSLFSIQILPSDFELFKKSDKYYSILQNYKKNYENQKQFYNQTKEKFDKFLISEQKNENQNKFNNIIEALEQDENNQKFCQTYNEIYSQILMHILSHYKNKPITQEENSKLFKMLIRLLHCFQPLQLSHHIFDPFKIEEKLLTSSGLEKEFKDLYEKLFNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XV66</accession>
    <name>A0A8S1XV66_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Myb-like DNA-binding domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1390058</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000139">
        <property type="protein sequence ID" value="CAD8204943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XV66"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF276">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_121, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="2">
        <location>
            <begin position="8"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="8"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="2">
        <location>
            <begin position="66"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="66"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8204943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="347" mass="41273" checksum="CA1560D3641C02E5" modified="2022-10-12" version="1">MVNQCNSDNKKKRKPWVIQEDKLLKKLVKKFQNERLAWKTISQNLKKHGFNRDTKACRERFSNHLDKSFNKADLTDKELDQLFDLIELHGNKWTHIAEQLNNRTDQDIKNKFYAHVKKIIRRLIKAAYQTTESSLIIARIQPLLISSIYCHDEEDNDKILKIDEQIKVLFKQLIRNNKKIEVGVQIDDQTIVQVKIVMNYLGEQNDIYLKKKISKQEQKILLKKLQQKRSLKLQQGLNRQSKIIEKILKKKPIFTTKNIKIENFMIYNTPGQIDQISIPFQEHKSIENMYQFQPYINPLISTASFYWSQSQNIFSIDPILSNNFLLGFQGNQNQYRMWSEKYRVTPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XVG4</accession>
    <name>A0A8S1XVG4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1390095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000139">
        <property type="protein sequence ID" value="CAD8205017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XVG4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="126"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8205017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="192" mass="23455" checksum="5257764979B1BD1F" modified="2022-10-12" version="1">MIFSQEARCTISQVCMIISYMILLKNGKIFNQYTDLQKKLIDSSFPEDDEFFKNDEPDLLELNLFILPNRIYGKRINRIKSRRNTNQQKLISLNNFKFIILIRISKMIKRQRRNYIKNYRLRSHQINNQKNYIKRLNQRKLRKKNRIEIIEIKTFDEEELTIKEKEEKEQNKTILKTAEIQKDNDIVVEYYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XW17</accession>
    <name>A0A8S1XW17_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1380042</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000138">
        <property type="protein sequence ID" value="CAD8204688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XW17"/>
    <dbReference type="OrthoDB" id="289586at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="31"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8204688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="165" mass="20317" checksum="DBE90CB755D43FAD" modified="2022-10-12" version="1">MNQPQRSLSNLGMSKSHYIPEQNRTSSAFYKRDEQSRLDQQLARYEEFKRNYKRNELKYSYYQQYPRDMNQSLVEERYQNSYNYRPNQNTKHLQTQQYEYDRDIYKKENKYDSPIRVELSYTAPSSAKKGVISDTAAYLREREKIMNSFMTQEEIQRIKRIYYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XWH2</accession>
    <name>A0A8S1XWH2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cysteine desulfurase</fullName>
            <ecNumber evidence="3">2.8.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T1410112</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.7" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDO010000141">
        <property type="protein sequence ID" value="CAD8205703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XWH2"/>
    <dbReference type="OrthoDB" id="10250117at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031071">
        <property type="term" value="F:cysteine desulfurase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044571">
        <property type="term" value="P:[2Fe-2S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000003">
        <property type="entry name" value="Cysteine desulfurase IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000002">
        <property type="entry name" value="Cysteine desulfurase IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00331">
        <property type="entry name" value="Cys_desulf_IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010240">
        <property type="entry name" value="Cys_deSase_IscS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016454">
        <property type="entry name" value="Cysteine_dSase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010611">
        <property type="entry name" value="PRK14012.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11601:SF34">
        <property type="entry name" value="CYSTEINE DESULFURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11601">
        <property type="entry name" value="CYSTEINE DESULFURYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005572">
        <property type="entry name" value="NifS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="9">
        <location>
            <begin position="12"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8205703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="411" mass="45953" checksum="629BC835D77F57C5" modified="2022-10-12" version="1">MRKTIFRFSRATYLDFQATTPTDYRVLDAMLPYLTNQYGNPHSKTHSFGWETEKAVEKARSQIASLINTQPQSIIFTSGATESNNAALKGLYGFYGKQKNHIITTQTEHKCVLDTCRYLEDKGVEVTYLPVDSNGLISLQQLQESIKSNTLCVSVMLVNNEIGVIQNLKEISRICHQRGVYVHSDMAQAIAKIPVDIQDLDIDLGSISAHKLYGPKGIGALYVRRKPRVRLQQIIHGGGQERGLRSGTLAPHLCVGFGKAAEIAQAELPYDIQHVDKLYKRLLNGIKEQIPQIQINGSLEHRYKGNLNVSFAFVEGESLIMAIKQVAVSSGSACTSASLEPSYVLRALGVQEDMAHTSLRIGIGRFTTEKEIDFLLDQLSTAVRKLREMSPLWEMHQEGIDINKIEWTQPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XXR9</accession>
    <name>A0A8S1XXR9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1430070</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XXR9"/>
    <dbReference type="OrthoDB" id="292259at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8206180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="263" mass="31800" checksum="D5DBBB762A244CF8" modified="2022-10-12" version="1">MQLPCMFCDHKGSLPELLNMALYSRYQTDFCQLQLRSPTTIRFQDQACFEELEEYFKFYYTFPYLKDNQKQLIEYYRYHRDIPRIFIKNITSQLEAYHEKQREIRYKKIKKQLGMHVQDTTRDNMIYSFKALKGLSELQTTRQLQQLVKQIKPIVYDKSGIATLIQKQKQQMLQSPQLKRLLKIDNINTATTQATKRTYHTYHTLQDVKKKGHKKQLTLCTEYSPDDRNDFYKRWSDVLSHRQLQYHKNKIKCTLLNKKKSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XZ78</accession>
    <name>A0A8S1XZ78_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1380096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000138">
        <property type="protein sequence ID" value="CAD8204794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XZ78"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="142"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8204794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="259" mass="30893" checksum="5BA79A1430011964" modified="2022-10-12" version="1">MNDIEIIKGQDILKFAQEMKKVNVNALLWLARQEQILLSDHQLEEIRKIESNHVLCPKQDENRAKDNQSQKENIYRFLSDVMDTVKYEVAIVMKLQLTSKMCQEEISKLNYQNIKKEDDNYIIKNLQKMFENNIGISKNVFLGELSNRRNRISEQFNEAKKEIMNNLKDSEKDKIYQSFLQKISLKDYKKIWKANLRVLNVYEALQNLQQQKKEKLSKRWFKFSGIKYEKPMKKLLDIQQKELSNDSPILIQMAQNKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XZP0</accession>
    <name>A0A8S1XZP0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1440052</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000144">
        <property type="protein sequence ID" value="CAD8206475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XZP0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8206475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="44" mass="5517" checksum="44654014EC92ADB5" modified="2022-10-12" version="1">MRRIRKCDEDEEVVEIEKQRLNEVNLKIEKCFVQKNRKQSNFRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XZP9</accession>
    <name>A0A8S1XZP9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1440057</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000144">
        <property type="protein sequence ID" value="CAD8206485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XZP9"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF68">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ATG1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="42"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8206485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="518" mass="59649" checksum="6B317DF09E42F918" modified="2022-10-12" version="1">MLQSNSIKERLKCKIEFGQDEEKPEIISLNIVDDAIKFEDRYETFQVLGQGAHAIVKLARKRETNDLYAVKITRMNNDEIYNNVKRTFYNARCLRHQNIIQEIELFINEKYCSACLVMEYCRFPTLEQILIERGTLREDETRQIIKQLLIAIQHIHNKGISHRDIKPDNILVNIEGNCELKLLDFGVSRRFLQKNNHIEMLTKTGNIYYCAPEIYHQAHYSKEIDIWSIGVVMFQCMTGQLPLHENSMSDQIQLLRTPEQWNFKNKVKDESLSAQNLLFRLLSADPKKRITAMEALTHPFIEKNHIYTTLAMLSSSKMFEDEDGFANKCKSLQTSLSINQKQNLHRALKTLHLGIEYQDIVIEDLIYELGNIHVVQKRNGENYPGLIQLMNSLGNSSGNNQTEVQNNNEKCNLDIGSSQSILSTENYTQDYFGDLCNIQSSVDLGNTGNNIQIEDKKKQTEINQFVNQIGSKIECSIDINLTDCIQESGLNNNNQNQLISTLDVLGIKKCNEEIENTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y2B9</accession>
    <name>A0A8S1Y2B9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Vacuolar sorting receptor thioredoxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T1490125</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000149">
        <property type="protein sequence ID" value="CAD8208079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y2B9"/>
    <dbReference type="OrthoDB" id="297237at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056858">
        <property type="entry name" value="VSR_TRX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22702">
        <property type="entry name" value="PROTEASE-ASSOCIATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22702:SF1">
        <property type="entry name" value="PROTEASE-ASSOCIATED DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25011">
        <property type="entry name" value="VSR_TRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar sorting receptor thioredoxin-like" evidence="10">
        <location>
            <begin position="31"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8208079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="294" mass="34021" checksum="6235CB15B513359F" modified="2022-10-12" version="1">MLYFIFIAIALSKDVKMNVNLTIGLDFQDNSYLDFFNNFSKHYDELQQNGLKLNIAQHLLPCYSCYTRHQFKQSEKNCLGGGRYCQYSDYVSGQIILKELLLQQCILDVLPQHYFNYTIYFGQQCKKSTMVACSQNYFINNNISIETINTCVDNSFNQSEDVNQEIQTNRILDQYKEMQYNQTQFSLYLDSSDQTDSSYQQFLKMLCQKYINVSISFCDNPIETPVETPVKQVENSNPLEQVFLFIFALIILIIAGSGGWTLLNKIQFGSSLVPRSRIAIPKLEGDHIIQEDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y2V4</accession>
    <name>A0A8S1Y2V4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1470122</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000147">
        <property type="protein sequence ID" value="CAD8207507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y2V4"/>
    <dbReference type="OrthoDB" id="300350at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002549">
        <property type="entry name" value="AI-2E-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 245"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="337"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="393"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="427"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="470"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="558"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8207507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="585" mass="67316" checksum="9EB9E84346A8A04B" modified="2022-10-12" version="1">MSSSQVGYLVTANIITAFGGGILYGLYQLAGDYLTTFFFMIIFAVALASIRISVVNNIKKLYAKNSERWSSQRIYFLHQAKFGIAFNLIITVLYSLSHPFTLLEYLKKLFTISSIPYVFVFYVIAIKLQCLTSVLLWDVLFWGVIDLLRIISYPFQRKNNKDEKGLLEWISKSKLFNTVLFASVLFGMILLLPIAIALIYQDIMIYKEDLIPKLQKYQIPNEYKYYLSADFLLSQFQGQYLSEAQIQQLSTYLNPVDVQDNHKNSVIFLEIFYGEHAIKILESIKECQDSKYCQAKVLLNNLDLLNDQIYEYLQYGYNYVNAILPQLQQFASVIGQFLIKVFSGSVAFLVKFIIFQQGVMYLLTESSFYDLISVNEKKNSFKGTLVQNISKHITGVFVSLFTIFMQHFMLSYMFLDILQIKLAFIQSFLIGLLSLTQIVDPGLTYILITGIWWLAGFLNNAAHEFCINDGLTMLFAIGFYFSSSSIYLNTFKKQLGDAYWSSMSVILGYISFKWQGLLIGPLLIGGVRTLYEILAWYWGEKEIMKDDYTIQNENQKIQNDPKAIENKGNTSKSNQSKKEGKKKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y327</accession>
    <name>A0A8S1Y327_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1430032</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y327"/>
    <dbReference type="OrthoDB" id="118550at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000020">
        <property type="entry name" value="SWI/SNF complex subunit SMARCC2 isoform c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SWIRM" evidence="5">
        <location>
            <begin position="76"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="4">
        <location>
            <begin position="267"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="6">
        <location>
            <begin position="273"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8206104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="443" mass="52837" checksum="99547D5F2E7D81BB" modified="2022-10-12" version="1">MYNRRPESRNSQITILLHKVSTGYEVVEVQTEQRKMIQKPSTQPIPPTVKKENTTTNITQETTQSKKDWFLQNWEAIIPAPARWFQIDSIHQIEKDSLPEFFHQHFHNDNSYYKGNYKTPLTYLKIRNSILQKWISTQTKYLKFTDCLNFISGDASSLLRVYTFLEHWGLINFQYNPNNLPNQGQVYQQNGTFLERVKLNFQSNQINFHSDPHYECHICDMKAYPFHQQKKENLASFQLQPLLLCNNCFLEKKYPKFLKNEDFSQLQQTQKYSPWTQEEIYRLLELVYKHKEKWNEIAKYFTKRSLTEIVKMYLQLPYSNLFPSLDKEPSIPKKPQKEHITFHNDVSNPIQLGVACFKSQLDKLKLPNIEVTQSQETEKMDENLKQMISVQMEKIEEQLLYLETYEQLINQEKQCVQTIQKKNLQMQVQVQTNNIVSDNMMFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y640</accession>
    <name>A0A8S1Y640_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1470055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000147">
        <property type="protein sequence ID" value="CAD8207382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y640"/>
    <dbReference type="OrthoDB" id="294447at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8207382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="112" mass="13136" checksum="90F3B3B52F07F778" modified="2022-10-12" version="1">MLNYIQPILDFSFYLFFGIYFSGPEEYELYYRIFAVCACLFYIFIFFPNKFKLFQVNSQLLSIGTGCVALASFVLSIFLSYEDIFFAYCYWSTLLISCGIGTFLVKTKYKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y7Z5</accession>
    <name>A0A8S1Y7Z5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1580048</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000158">
        <property type="protein sequence ID" value="CAD8210185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y7Z5"/>
    <dbReference type="OrthoDB" id="301594at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035721849" evidence="2">
        <location>
            <begin position="21"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="53"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="256"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8210185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="357" mass="40593" checksum="871D4D5990D1DDA3" modified="2022-10-12" version="1">MKSIILIALIAVAFTARVQERNMAKVQADLAKSNYGKALLHLVELHSMAGGPVQELIDAIEELINDLEEELEELEFNFQIRTNEHNALVVSLEQDIQDAVIDVNNTQDTLDNLLYPRRAQIQDKIDSVLGYQEDNRKNYDEAILVREQEHEAFEAQVAELNDATASVDDALALLASLTNPSLLQIRRFQNTLKNIENKIRSRSRMAPMIKALISLASNQNFSNQDVLKNIVDALNDFRNAVVDQINDLTAQEAQDVVDHEAYLEQLDDEHREFQRQIDRATVDLTATNEKIDSMVEFRDQRAADQKQYTAELELENNTYAEETDIYTNTKNEFTRELGVSEQALALVKSVDFSNIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YB23</accession>
    <name>A0A8S1YB23_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1570063</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000157">
        <property type="protein sequence ID" value="CAD8210007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YB23"/>
    <dbReference type="OrthoDB" id="301236at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8210007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="300" mass="35205" checksum="E86ED18371AB1B6A" modified="2022-10-12" version="1">MLLRQYIIILLFRLRNINQRNYLRIYFNLLEQLSQNNIILFNKLLNHYFLNKFEVKLNIIIVIPTQLKISISCFNIKISNSPINDYYVTFPIITDLFIHNYPKQPRNPLNNNKLASQRETALNCSTEQYNQYKMEDIIPKKEKYCKIAKELTSYERELISTQANEVVTAVKRAKHNPKKLRQVNSSLFQLNKIGGLEIKITPKRQNKISRIVLFVITSFFRMYAKIKVVHKGLVFIKTTASETVTNFNDKKQERNPTPPMQPRVISFLSVLLEEGYVLFGMNSAKISMIIKVKIDLQQII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YEF0</accession>
    <name>A0A8S1YEF0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1660117</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000166">
        <property type="protein sequence ID" value="CAD8212150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YEF0"/>
    <dbReference type="OrthoDB" id="313090at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000089">
        <property type="entry name" value="Cyclin, N-terminal domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000183">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0195">Cyclin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="3">
        <location>
            <begin position="139"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="3">
        <location>
            <begin position="239"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8212150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="359" mass="41211" checksum="CE902E4766CC99C7" modified="2022-10-12" version="1">MKDMSKKIFEKENQAEVSNNPRHQNKSLTSLVSLSLIGGNKKNEFKSMELKNNYTSISSRPLVFCKPESKIKTLTTSSPQKQSPIKPKQVETQSNELCKLINNYSEPILDYYLELNNQTPKNFLSNHTVSSNLRAKMVDWMVEVLTSYKCKDQTFFLAVRLMDSYLSKTTQKHIPQDLHLIGVTTMFMACKFEEIYPVKLQIVHEKIAHKKLTKDEIKEKETQILTTLDFSFIGITVLDVITMVLSILNMNQQLYQITLYLAKLALYDYEFANNHTYVQFACAALIVTTKIVEQIDQSLSSEIVIPLIVSTLKMDNSDAMDSANKLLNLAKGFDKQYPNLENLKKFSKFQLSDMLTTQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YES4</accession>
    <name>A0A8S1YES4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">OPA3-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1650006</name>
        <name evidence="5" type="ORF">PPENT_87.1.T1650008</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OPA3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000165">
        <property type="protein sequence ID" value="CAD8211724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000165">
        <property type="protein sequence ID" value="CAD8211728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YES4"/>
    <dbReference type="OrthoDB" id="2129069at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019216">
        <property type="term" value="P:regulation of lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010754">
        <property type="entry name" value="OPA3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12499:SF0">
        <property type="entry name" value="OPTIC ATROPHY 3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12499">
        <property type="entry name" value="OPTIC ATROPHY 3 PROTEIN OPA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07047">
        <property type="entry name" value="OPA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="115"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8211724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8211728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="177" mass="20680" checksum="CA2BAC3B9087EE72" modified="2022-10-12" version="1">MIPIQKLASLLIRTFSKPLSNQIKRYALNKHRSKKPSFVKTAFVFLGNKYHNFETFINRKSIGIKSQEMFFKPLSDEAALNKGSDLFADIFVYACILGIPLLELYKSQRDSAKKSQEQDTALQNYQTQIDNLKQLNDQFQIEYSRIVAENQYIQQSLKHFEKDIDDQVQHLQKQLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YF90</accession>
    <name>A0A8S1YF90_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1660056</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000166">
        <property type="protein sequence ID" value="CAD8212043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YF90"/>
    <dbReference type="OrthoDB" id="668540at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010608">
        <property type="term" value="P:post-transcriptional regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF13">
        <property type="entry name" value="PUMILIO HOMOLOGY DOMAIN FAMILY MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="412"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8212043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="490" mass="58225" checksum="012FA30E0A328555" modified="2022-10-12" version="1">MKRAMFLVDQKKNELIGKVGHLYSPLKDYINPTTPTKYNQIQNLLCNWQFYYFKILETTPCFMLDDIYPLFDLNEQFPIREYKEILSKYVQKETEPTHDFNSRKKELKRQFHMSNFLFRNWAILNYTQIICPSILISILNVHLFILHEKIKLVKLVKCIPVDYDLLDLNIKAFVEFQDFHIIAQKPFLHLTYSTTLKQKKNPSILIDYANLKQYYGKQINYIIPFLQGRIQVQLYIIDANLIQKGKDAHTYVYYYNAEQSTVGDYFSLESGGVCISMQLEEDIIQLSIKSLQIALPTKLTQIGPIQTRITYQIFNLKKLQQKNGLKIFRILNRLEVMLLIQKHFTTCTQKELDQILQEIESHPLDLMIDPYANYMFVSLSQSYAPHQRLYILQTSLVSLISYKQEEQMVENSIKNNIIPLTIDSQGTHLIKKIIVRFSEDRLNYIFHKLMKRLIYVINNQFGLCLLKDLQIQKKFRKIQYYYQQNEGSFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YGY7</accession>
    <name>A0A8S1YGY7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1700029</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000170">
        <property type="protein sequence ID" value="CAD8212668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YGY7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8212668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="113" mass="13380" checksum="8E61CEFE6DACED01" modified="2022-10-12" version="1">MNSQDDYNFQGSYNIIAKNICVGCQMEQQVQKLQIQKELTLEVFLTFTSTKTKKKIYQANLFNDIKSKTHYLQKEDQNYLIECLEQFEENLINQFCQKQKPLKEIVSILNTTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YH28</accession>
    <name>A0A8S1YH28_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1660043</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000166">
        <property type="protein sequence ID" value="CAD8212017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YH28"/>
    <dbReference type="OrthoDB" id="291817at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8212017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="128" mass="14547" checksum="EF90F4CF0356E23B" modified="2022-10-12" version="1">MGCCQDRPTIILKGNELKHPIKEPSIVLTKRAQILEPIKISIMGDIDQMDFIDEFPIQMPSITQIDSKLPNKLMISGDLKETKDFKSTLISDTIIIDMRSGTLLQSPLKQMSERNSSKNLNKKFLEML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YHL3</accession>
    <name>A0A8S1YHL3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1750042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000175">
        <property type="protein sequence ID" value="CAD8213219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YHL3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="62" mass="7389" checksum="F93B74E0C74BC6F1" modified="2022-10-12" version="1">MLIFDISEFISLSLKPKPMDIKRFELSWQQLKSSIAKFHKSNLRFGTRQIKNKQAFNIFEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YKL3</accession>
    <name>A0A8S1YKL3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PUB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1720045</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000172">
        <property type="protein sequence ID" value="CAD8212957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YKL3"/>
    <dbReference type="OrthoDB" id="336240at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09212">
        <property type="entry name" value="PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018997">
        <property type="entry name" value="PUB_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23153:SF38">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23153">
        <property type="entry name" value="UBX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09409">
        <property type="entry name" value="PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00580">
        <property type="entry name" value="PUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PUB" evidence="2">
        <location>
            <begin position="22"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8212957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="189" mass="22039" checksum="B33D437FBA86F392" modified="2022-10-12" version="1">MISSNQTKSLLEVIEKNEDPSAFADALGLLEKLLTNIINNPNEDKFKHIKMTVKTLATRLFNIRQMAELLTSLGFIQLDQEFYLPDEELAKLLENFNTIKWQHILAQGRVDGPQQYQKAQEIVRQQQEAQRQYEKEQREKEKIQQQMKYDRQERSLVKEKDSKANDLQFGAKVKTCEQLGINKNTGKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RX60</accession>
    <name>A0A8S1RX60_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0010403</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RX60"/>
    <dbReference type="OrthoDB" id="303646at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="328"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="376"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="526"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="338"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="376"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="388"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="541"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8132022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="630" mass="72406" checksum="3643CE05E5AA9FD8" modified="2022-10-12" version="1">MSNKKQFTGGKTSRSNSKQSTSNPLSVRKRFSSLCSPPNNQKSTPCVFCQKFKNCKEESYAPLLNKALFAKYSSSQNYYYSKDINDIIEEESTPAVVFYRDLESMIEEEEYLKRSYVKKEAIQKVKALQEYYKYHKDIPRLFMQNVYLTINKFHEKKRRLEYANIKRKLNIPDEDQPSKKKEKKKQKHSDEDLTIGQLKYLLKDLKLDTNSYLQKKVDISSSVQLREFIQQVGQKYDCLGQISGLLSIDQSNSFLLRSQDLSTITKNLKSNYMTNLKPQINKSSQLQNFDSKTTHQVSYQGSFDKININRLQFNQGEVSSLKAENSIKAGEQTKRSNQEQSPLRNSQQSQLMQKVYSQIEGFANFVQQQQNIQKQQKANNCQKSNSQRKVSGNFNTTESQFRLPSRQNSTQKQSADLLSYQVNFLRTRVASQLQKYEDDNKSKQPLATDRVQRQSVYTIGEQNLQKTASKNIQKLPQKFTQYALSTLKGLQVKNSDQKKKSTLISNNNLQAPILNAQLMHYRTKSQDNLGSYKGSPPKYPQKHDNSKDPLKITNNICVQGSTKKGGGVYNINQNNIVNIYIEDLKSSTKLKQGGSQTARINSPLKSNPKGIFEVYHTQRSNQSPVSKKMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RYB5</accession>
    <name>A0A8S1RYB5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0010582</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RYB5"/>
    <dbReference type="OrthoDB" id="302039at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035842229" evidence="2">
        <location>
            <begin position="18"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="264"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8132380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="350" mass="39609" checksum="781FA7E8036EE824" modified="2022-10-12" version="1">MTFKTLIVLLLVTMAVATESPALEKLRNKLDNNEYNSQLVELLELSLAGGQLDRVFELLQKMVDDLIGQINTANLDHASRMAAFESSIEQLEANLASLSSDVQINNRKIGEITQEISTLTSTSVAVKKQLQTVNQREEQIRDNRQKEIEALETKQSASSKILAALEDIHDRLVKAVLSNTGSFLEENEKKEMIKQVKNELGRKHPLALLLEFSSKFDEATAKRAIELIEQIIASIKEGQQNREANQTAVEQNFNSLVNEVTVVREKLEQDNQKTTSALKNRQNDLKITQRRNKQLSQNLINTQQLLETTRVQKDLYDSNFRSNASKKEGQLNSLKTALQILRDNEQALKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S009</accession>
    <name>A0A8S1S009_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0010424</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S009"/>
    <dbReference type="OrthoDB" id="284647at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035909647" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8132064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="306" mass="35587" checksum="90C9BC367F1DCD09" modified="2022-10-12" version="1">MIKLSSFIYLLVSQILCLDQWQCTCEQLMTEFDCMTQQKNRFRLGEIDQSSICFWYQEKCQNLMNYLSDVSCTSLNNQECSFVSESLFTINPVCAWYYKADGGQCTKFTRCQDYKYQSELIAEVECSNLGCNRIKNQCSFDYRGRQEQYCSEIGDQKTCDITFLKDKLTICQWNTSTKKCGGFNFEQCSDLTTEQNCDFANTFCKWKDQLCIERVCGDKELEYKSGSQICISFYSLTNKKYMQCVENNNGDCIESDPETLLPYQCNYFSRLTYLWNTTTSSCTPCLYVGQSDIIKVAIIILFVIFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S173</accession>
    <name>A0A8S1S173_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0030050</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8133936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S173"/>
    <dbReference type="OrthoDB" id="354826at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05578">
        <property type="entry name" value="STKc_Yank1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000454">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000654">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF374">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="134"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="10">
        <location>
            <begin position="393"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8133936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="491" mass="56817" checksum="0817638F8BD05633" modified="2022-10-12" version="1">MLINSTSQRKLKQNSLSQFRSPIIIPSSQRISPVASGLNSLKNLHNHKGSQIDINNLTRIIKPTKLSESVNNLQIQVWLAKAKENIKVTAETKLQLPKKHIKFPSQQFTNFKDIVHTSISPKLNRETLFSRNAFNFEFVIGIGGFGKVWKVQHKKTSLTFAMKEMSKALIVTKKSVHSVMNERQLLSQLKNSFIVNMNYAYQDRENLYLVMDYMKGGDLRYHIGKMRRFNEQSTKFFIACIVQGLEYIHSNKIIHRDIKPENLVLDEKGYVHITDFGIARISKTENNSDTSGTPGYMAPEVMCRQNHTYAVDYFALGVIAYEFMLGRRPYLGRSRQEIRDSILAKQVQIRKSEVPDDWSLEAVDFINQLLQRKPQNRLGYSGSQEIKDHPWFAQFPWDKLQNKSLTPPFIPNANEDNFDQKQIILEDEENNELIQSNILLLRDQSIQDQFQGYEFYYHANSSSTEQSSGSSTKHSRNLSERIQFADKRKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S873</accession>
    <name>A0A8S1S873_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0040128</name>
        <name evidence="2" type="ORF">PPENT_87.1.T0080395</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S873"/>
    <dbReference type="OrthoDB" id="288849at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8135249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8139986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="433" mass="50174" checksum="CE7DDDB4704CCDA3" modified="2022-10-12" version="1">MSITVEDLAANIETVLASLGDEKMVNWEVVEQTEHNLMKHFDQLFERLYTLKDSLQRVFLINIKNLRQYDAKDLVQKVKDKKLEPVVLKSLTQYAFKNDKDELLLNPSLIKGQVNSIVLPWVGNNLSVTDKIKETNEWLDQKLAELEVFKPKESQETTKIQDLQPKNEGNDKNNYTFKSDKLNEIASQIQTMHIPNTNNMISIKTYTQIVEGSVELAEPEFIRITLENRKARREVLYSDGAKYVQLLLDYVNDIENLLMKAQEEICKKIDISQQTLEQSEMLLMERGLGQHVFMLQASARQRIKDKLPKQKQVQMNATKEIIRYQIKLLNEKQDFLKNMIDKLPTTYESGQLVPMVLNLVMGDMIFEEHSYEEEDYISNLENPQLFQDPDMMELLKSIETGVVQLLGKTAFAQPQGGMMGNIPPQMLQKQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SBI8</accession>
    <name>A0A8S1SBI8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0050187</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SBI8"/>
    <dbReference type="OrthoDB" id="303855at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12621">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN CHORD -CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12621:SF7">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8136512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="475" mass="55578" checksum="2B833B667004F7B2" modified="2022-10-12" version="1">MNYLSFIDIFGVEFRQQIHLNVKSQKSVFGGITSLLILATSLGYLIYILNEWIQFRLLPKSTSTMKANLTSELQYDEDAILFEFFYWKYSEQQSDPFNQQNNILTPLGIYFEDGQPTTIFSFLSQVESLSYYGTKKFGLNQFKLSQNSNGTSKENVREMMLVFVRCNQTYLSENQTCASQQEIDHFFDSAVNYMAFQINLKQFNSQTEVFEFVKKTYYFSLDKAISTQSQILLKQTQAFIDSGIILNSIQEETFVQDAYILTTSASINFWKKIIGMDTYFNMIFRLDPVSEELNIVYPKLGEVLAQVGSIVNMLMILKYIILYYNEKLLEHTFIDQVLSFYFTDYKQIKKSKVNYDKKACSILVEQAQKRLVYINIIYELSRIQLFLQNHFGRKKLQESHSMGILLKPPNKNLNQDLEQALAQCQEENLEDENQKFHIQDFFLLSQPKQLLNQIEDQSVRKHDPIITPNLNHSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SCA0</accession>
    <name>A0A8S1SCA0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0050337</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SCA0"/>
    <dbReference type="OrthoDB" id="302289at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8136769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="343" mass="40486" checksum="CA8F5A0C55698D16" modified="2022-10-12" version="1">MQTIQLKNLLKDHKELKKQFTLKRKFDITSLPILVRNKSCECLECGGQIIKQIEEHKINNKVSPKYIKKFIAQHEKFQSFNFQSHKVQTTQNMVFLDIAPKRIQQTFSNSNEQPLISPTLEQPKRDMRKLSRLIEKYDITKTEIVQQQYCKTLPDVVYKNKNLCQYLQRQKTQHYSYIKILPIGDEEQQNSGTNSNHLKNSSQRTPIRNQVYKELNESTILRQLQTPILQKSKFQNYPIIDFADGIRKAMGKIKEIDEYSNNSKIVSTQFNEDTKNKKRGSILLKSMLDCTKLLEKNQKTLKQLDVFVRTKSLNRKLSKYEKNYSPSKNYSLSKVYLPKIIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SI61</accession>
    <name>A0A8S1SI61_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0060442</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8138084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SI61"/>
    <dbReference type="OrthoDB" id="304280at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="16"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="64"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="129"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="214"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8138084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="823" mass="97669" checksum="EAE71D19C7F4BFF8" modified="2022-10-12" version="1">MRATLFQQLTKIRAESAKSGTNRNKPILDTSDLLPRSSFHRKRRQDDQNNSFNEYSCSFRFPDKERYEQEIKQLKNNMHHLENENIRLKTRIHQMEDNQLKQEKIIQELDRIGPVKAPLLQALNFSTAQTALKKEIINIRQELQKKDKELQTLKKTQRLSQITELQIERAAFEEETIRLRQQIDSLFKASLFNLQQNNVDQIIQERLFALVAQIQQYLMQRRELEKVIDRLKKECLKYRSQQIDAEYRSRREIKKLIAQLKEETQNNQKENNKELKQNPTKADELQILTDLMFAKQEIKSKDNEIERLNQIIFDLEVQIKEISLSQNQEAQDLQNQSQQLDATSKSKQMKLSSPVFATEILQEIALPIKKSIVIQEQQSVIKQPPKRRIIPVKFENIKIIGETLKYKLMAMDIGIQQIDDYLFEGEFMTIKELKDKLRLYPFNLTKDEAFLLARYIMEGENDVYELEDLAQNPTPYIRSVFRKVLFNYKLEIVKSQFEYSEKIKDVLTRFKPFIISNIKQLHGKECIRVSKSQFIDAMASLNIELNQFEIDYLIMQGILESRSVESLNYEEMLKHQPIKIEDQQLSFLLTEITNENKQKVEMSVIMECPEKVSEIEQQEQANVSNTNFNEEQQLDINMQGSNLNQKMKNISKNYRQILIQNSDNFYLIDIKVLIKFICQIKNMSFRVEESPQFKEEELNLSKIYQYRESHFSGFSFPSSVRHQKRQTFQASVETKTPTPNQTNIENSPCELSYEIERMVKEREQLFTDVQYYEPKVTFNIDESPQRIRIQRNSAPMPNTQLYHLNQQIIDAEHTYNQYCYQLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJF1</accession>
    <name>A0A8S1SJF1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0070148</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJF1"/>
    <dbReference type="OrthoDB" id="165382at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24134:SF9">
        <property type="entry name" value="ANKYRIN REPEAT AND SOCS BOX PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24134">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN DDB_G0279043"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="284"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8138504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="388" mass="45976" checksum="032742CA614B52B5" modified="2022-10-12" version="1">MFVEDYYRRLIKQQCKLNSKEIKTDRPWYPKRQQNETKKEEYKLFMLPPNKMITLLKKNKISAQNEKIKEFLHNHSLKQIVDFGETLQSTLDMKVQRPSIQIPIPKNNNMGRQLFPVKRRPLQLPSTRVITEPSKKSSSQCRSVHKFIRLKQSDHSNEELYSIITKLNYSPLHVDTTNICKKQTQPQKIVQQQPYIKPEIPKSVLPYPPRTKKQLRQFLLRALKKIKALGLTIKYVMKHKIFSKKPYEKINSKEFIHAAKQNKIEDIENYLQINPYLVFEYDFYNMTALHWACKKGYIEIVEILLKYHSDIDGVDILYRTPLILSIQENHLDVTHLLLTNGAYPWSTAITDLKSILEENERAKILLTRVRRLQIMAKWTQSGEYLNLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SM67</accession>
    <name>A0A8S1SM67_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0090372</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endomembrane system</location>
            <topology evidence="5">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SM67"/>
    <dbReference type="OrthoDB" id="66964at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052606">
        <property type="entry name" value="DnaJ_domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44653">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44653:SF2">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="30"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="253"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="7">
        <location>
            <begin position="50"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8140968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="291" mass="34575" checksum="6DDBBC9BC9CAFE8E" modified="2022-10-12" version="1">MLLNQIGVIFFNVAFLSLILGQFKGSIQKILYILIVALEIYIYLTYKPINFYEEYKITRKFEQSELRQAYKRIMLDYHPDRNTQITSEQFNYLKSINEILKSDEARLLYDQFGVVVESNINPLPTIIYRIKLTRIFFYMIIFLIQLVNTQESKNQSKYLIFLILILGYVDFQLVEQPQYFQNSSFFASLPLFEFLNIYRIVVTIIISQQLADIRNKQINRNNQLKQLELGTINELVKEHQEFLQSQNSTKQKFLNGFLMIGLTLYSLSSFLDIQALILKTLNYIQERRQDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SNT7</accession>
    <name>A0A8S1SNT7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0080464</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8140124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SNT7"/>
    <dbReference type="OrthoDB" id="10249433at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF81">
        <property type="entry name" value="PROTEIN ABHD13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="4">
        <location>
            <begin position="278"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="694"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="68"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="705"/>
            <end position="714"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8140124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="760" mass="89056" checksum="66CBAC1766E8C0AE" modified="2022-10-12" version="1">MQITDEQKKIMAIIGVAILLLGILYLIDCLIVFQFGLLGHFMFFVGLYVFIKNTIRLFVFPGSYWLWRRNVENRYAKALAKRANKRMEDLQNLIRKLLQIPDTKEIYVEDLVPIKKTLQSFLNTFSSLNCRKELNEQQKYLFGLCQQLEQNLKDFLIDSKSLYDQLDNPQEVINYCKLTIQVDSEQCSNLLLILDNLVNQLQEIYIPKNIILEAERWIFNKTLGTVDQMRVELENTFKTRRFKVQGYDGKQIDCLYIYANEDDTENDRAVIFCQPNAGYYEYMYYESEWIDYYLKRGISMFLWNYRGYCESQGLPNTKDIMKDAESICDYVTSQLKVNQLIFHGESLGGMVACHLGRVRQCNLLFADRTFSSISKIAQVGFSKYASFLFKLLTSWDYNSAKSYVECQSYKILALDQKDEVIPFLSSLKVDVTKQIFLKEFGGSYQNDENFKQSDHRYTFCQKLLRYIPWIPKSESTIFYDIWYSQLLNKQEMLTFFEANKRIQYIMKDLQTERSKKTLDDSYSAYMNESQDIEKSFSQNQSSTNDIDEVTQLAGPYTLSEKEKSNENLLKFVNKVYECFESFETASMTLNDVFSAFSESQQYDVFLDYLLSIFLWGSYIPLKNIPKKKMNTVVCQKLAFARFTSNINKIETLIKNDKNNLDSPNGTRLIQDLELIIQRLKLIRENFKKTLKNQHQHPSHQSVQIEMKDSEERKSTSKINESLKTYLGSFIPLGCGHNGNLNQTEVEVLDFHMLQAGVVKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SNU4</accession>
    <name>A0A8S1SNU4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WDR19 first beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0110005</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SNU4"/>
    <dbReference type="OrthoDB" id="338608at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057855">
        <property type="entry name" value="Beta-prop_WDR19_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23389">
        <property type="entry name" value="Beta-prop_WDR19_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="219"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="WDR19 first beta-propeller" evidence="4">
        <location>
            <begin position="233"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="261"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="303"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="345"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8142193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="480" mass="54281" checksum="CE57C28B9D155D48" modified="2022-10-12" version="1">MQGSFQVKEAIADIFDKAKDVDIQIFGVLLEIFGKEKVQDCIQYLSDIGNQRQQESQILQQVGNFTQTDTEQILSNFRNDMKQIRDVLRKLKDHDFNYKDFSSEENEESKLNLIKSIKDNKRMIEFLQFLVHLTSIDNTLIQCGSNSLHILIKMKVDLRNQNFEKITIQNTSLLGANLVRCNFSESQFKNVNISGTNLNGALLFNCKWRDLRICEVQKLHGYCDCVKTVCFSPDGNTLASGSADKSIRLWDVKTGQQKAKLQIHRGSVWSVGFSPNGNTLASVDGNGTVILWDIKTRKKRAKLDGHRGTVYSVCFSPDGNTLASGSDDYSIRLWDVKTGQQKAKLDGHTREIMSVCFSPDGNTLASGSGDNSIRLCDVKTGQQKAKLDSHTNNVNSNGIEIQSDDNKYKDIPTQFKIPLFQNKLLPESNNITIQRISQTPLFQAQGALILKGEFVNYKGYNLRYLLKSKGYCFLEDYKKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SQL9</accession>
    <name>A0A8S1SQL9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0090289</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SQL9"/>
    <dbReference type="OrthoDB" id="299052at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="613"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="613"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="624"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="642"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8140804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1040" mass="122665" checksum="543008005C580212" modified="2022-10-12" version="1">MIFAEALGEDDEYIRLQKISLGIKEVSLNLRNMQIITDHNILKSMICSYPFQQASIELLMSAFFDYFKHKLFLLAFYRALQEVSELQEYREFLGNRSTRNLKMTINGNVNKIIEEQQKQGSIPFSKQVIEKLLIAKRFACSYLKRFFQLAIQINVDLEEMSIIFKQFIIHTRCHRFQQQLIDQFFDCKQSIFYEFPWKNKYDQELDDFCNRWTIEVAPKWVLDRKKSMSSFNYIATEKQIKIEQEDEYITPASKVESVADYVEIMQKIICKPIEMVTQHDQLQLHNKDNIEILIKFIIDPLETELIDDWGLLFQRKEIESNDYKTTFKFSTISMEYLNTVRSFKSLQFLTQKVNHSRFYLTIQEMTPKIFEQILENLNSQTIQMNLNHLAILIDQLLLICPKASIMKIIELDFLFLFLQYSSNTYIESLITDILDLTVDKYKLGYYIQEQIWKYIVETQWIDCLSNSIFQQNYSLSNQKLPSIENNEQKSQILNILSKFKDIQKPIEIVKISTLDEFIGPLGGQTYQQNSEEHLQLPENISYAQLLEHDVDAIRAYLDERRMHKPINYSRKQSRKVESQMSTYRSPTISHNRNISLNVPILPQINTPKSISNKQTEVQSFLNDSKSKRGSVEKQEKHDKSISSKTSQWAQNLQQTSRSSLSSETGFSSSKLITLYPSGNQKANPSQFEINLTDFYYNTNTYEHLINLLEKIVNVIIIHQMRNKNNGEHFSNFVFNQDLVLSLFKFYLYEINQKKDISFQCGKIINQIYFLGKKYGNVEQQEFLREIFYQIIEYLNKVIININRLNSDLNFITQFILFQTINNGFAIFEPYDATKLNRNIYKFLSETIIHLYIIYFFKSKQNTLYQYQFVEFINMIFEKAPTYLLQNILFNVGLISSLYNAYTTFYANGFKSHSYNESLFYYLIKLIRVIHNNLNKRELTVILSSLQPLDSWRCLIKTLPSSQIIKEKKLKTQEVELQKSTLESQLNNRQSVLNESRASISLTLNNRKLSQKQSTPQKLSARIQQLQQKGISSQSPSQLAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SSY4</accession>
    <name>A0A8S1SSY4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0110074</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SSY4"/>
    <dbReference type="OrthoDB" id="308885at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="370"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="370"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8142307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="389" mass="45450" checksum="39F414A3DF5FC583" modified="2022-10-12" version="1">MSQIIQENNSQVSNQAQESDQEMKKQQYQFIQSENQVLRKGKKHTIHQFYKNQTDRYLETLKSKKSNDTSEIMNNSFQAPINSEQDQEQNKDQQLFIRKNDRKQSTKQFYQKNVMKMLEKFAKKENGQTIDDKINSNQTNQNDINYSEKRNEESQIYMNNSLFQESIKLIELKKNLEIMQSKQSPLIQLTKDDILQLQNQQIKQDTTQQNINVFFNSNTNSKVSAMKGNRRNQSIQKSQPKVQFPFLEQNNLDQEINQNLILGQNEEIKLDLSKLNAINRMEKKKKKNETQSANSKRSIRSNWTNKTFKEKAEEVAVPAVGTAIVGSLGVMLAKNVLKGKQQLLGIIPALACSAYYYYKVKKQNEVEMEFEDEEDINSEEEQDSTRLEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SWR3</accession>
    <name>A0A8S1SWR3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0110369</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SWR3"/>
    <dbReference type="OrthoDB" id="302028at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="204"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="292"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="151"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="204"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="314"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8142852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="332" mass="39666" checksum="A511DFF7CEA1B6AD" modified="2022-10-12" version="1">MNQQSFVQVTQEYDQVNKRVNSLKFQLRDIDQKNFLLQTKLKDLRQISPKIQKSPITQLQEQLRLDRKQIIEQFKPIVEKEGIYLRNERLKTESQLQQSKIWDIQQKKMKVKMIDEFDSEVTKRRAEYQEKKIQEVRQKQLQEMQMNRLIVNKKLLEITKLKKQEKELIEEIQSAKTKEQQLCNKFCNVLASNDNSVILPSIQKSNSRMSQNSNSKYQNKGCSPFQKSDKVFDRLQRSETRNDNSIIEFEENRRLQLETRKKSQSIDQLNIGCQTSFLNKDQNFESVQYSQNNDSYLNRQKDKTLEKSQANQTSEERKDQKEKNKLTSNKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T4S9</accession>
    <name>A0A8S1T4S9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0180054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T4S9"/>
    <dbReference type="OrthoDB" id="308755at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="392"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="467"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="315"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="467"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8148415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="913" mass="108434" checksum="DF871E030D4D8B32" modified="2022-10-12" version="1">MQQIQIQTATQDKAAYNRNAQKIIDQLKANDLELIDATLEYALQVFNSNEPPLRKLLILRLIKDAIVIKSAKFINKYIQTRPLLTFIGNICTHKLGDSNPNRGADLFQGQDPQSSIEFLNLALELVDFLATTFPKDSKGAPTKFKIQFDELVQMGVKFPPRSSLILPFDPNKASQEPIQKPLNNNKERDETMLKLNQSKQNIYQDIEMINETLVNDSDMSYINEVLVTYSESMNVIEKQFENLINKAQQYDGVLNEDEIAELYALKDFVLKFQQYYYAYLEKECTPVAYNSLKKKTLAHIQKIQNKDFDYPPPQQQKQSRQQQIQQSQLQNEDEMLKRAIEESKIQQQNSQIQQKQFQEQQKQEEIKRQQDEELRQAKLREEQIKQEYLRAEEQRRENERKEHERKEHERRENERKEQIRQEQLRLEEKRMEQQKLEQLKQEQNRLEAIQQQQQQMKQKEIEDYWNNQFQTPNPDNQQYMTPTKLGSYTMPQQQQQQQQQQQQQQQQQQQQQQQQQQQYAQSYMQSSFQQQNGTINNNFIYSTVDQQQQQKQPIKQTNANQFEQSYNNSQIPNQQYLHGQNTREQLPMNHITSNIIKKKEFDENFDGLSQSTLTQTFFPLKMDQQIPIKQYIGKFVKYDNSQFELLHTYGLTESQKKRFKIATIKGRAALINSPQLQIGIKQALQYVPLKEKNYLKLTLYIGNKTQSNFENCKMQFEGDNKQFSMWLKPDKIVDKIEAGQQIQQEVIGIFKQYENYRLVNVIFSAEWANKQISSNFFLTTTLISFMDFKDISLSIFRTKWRQKKTSILRGETFLLNPKIVKTGQYLKKIFPKLCEFNSYQSFQDQSFDYFSDRQLKYISYKLCGIFELTNVNQNYMIKFIVLPNMQCTIQIIGNNEHLCKQMLNTLNWILGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T695</accession>
    <name>A0A8S1T695_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein kinase domain containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0170259</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8148020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T695"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000726">
        <property type="entry name" value="Calcium-dependent protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000634">
        <property type="entry name" value="Calcium-dependent protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000382">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="34"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="349"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="471"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8148020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="522" mass="61003" checksum="19F8AB7A83F9C272" modified="2022-10-12" version="1">MGCLQSKKRNRIVAMQTMNKDQVFTSTADIHQLYNFGKVLGVGSFGKVLLAKMKQNSEKQYAIKVIDKRRVKGKEALLANEIFVLQQLDHPNIIKFYEVYQSPLYFYICMDYCQGGELVERIAKQQSTLSEGQVQTIIQKICSAIVYIHDLGLVHRDIKPENIMFSEKDIYSEPKLIDFGLANKYDTTHIKRLKTFVGTPLYLPPEVIDGEYDEKCDVWSLGVMLFSLLCGYPPFYGKNRAQLYENIKSQALVFDRRHWRNISEQAKDLIQKMLMKTPKKRLSARECLKHSWFEMQFQNVKVHREKSQQLLTQTHEDNRTIYQMLKMFRGGAKFKKEVTKVLVNQMNEKELHHLKQVFQKIDVDNSGTITIEELREALQQEGSPASLEEIEQIIQTIVLEDDEENNNLEMDKEPSPLVIKYTDFLAACIDERKVLTREKLWSLFKYFDTLNVNYITKEDIKEALARHGRQMSDEKIDQMIYEIDPNHDNKISFDEFLQMMGVAGIEQTMNIRDEVKEIEIQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T6P0</accession>
    <name>A0A8S1T6P0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0160216</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T6P0"/>
    <dbReference type="OrthoDB" id="298354at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00195">
        <property type="entry name" value="UBCc_UEV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8147116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="340" mass="39987" checksum="4B21EB29F8956F9E" modified="2022-10-12" version="1">MDNAENIITEIFKNEIQVKRLKKEIQSLTQLNQKEFKITLKTLSPNLQIIVEMPPIDSLNSDKPIIFALYLDNRFPFVFPKVHILSQFTKPTLSDGRDYLENIISGPWSPSILLYEIIKMFPQFLETIKNNQNNKDYLLQLGNYQENQEFDLNIGLQNVDYLQCQQIINGKQFPRTILISESHLLNLEYQNKESILLNYYCMMNLQKIEKVQKNILLNWQMNDGSLIIQTLTSNNLDQIYNIINSYKLGQTQKKFNQDDVTLQKFESIQINELLQQMNFNEIELSKNLNKISLHNLMTSYQKAIEYYSAFSDEDYKQYVIRLQTLLGREDVQTILACPVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T8F9</accession>
    <name>A0A8S1T8F9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0180138</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T8F9"/>
    <dbReference type="OrthoDB" id="62956at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035725304" description="GOLD domain-containing protein" evidence="9">
        <location>
            <begin position="16"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="10">
        <location>
            <begin position="24"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8148583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="195" mass="22644" checksum="B1488A3B4AF676D3" modified="2022-10-12" version="1">MKLLYLISLIYMGHGLSVQISQKPTCFYVKSYTGDTYVTVNYQISGLDENKTEITIESENGDKLVKKQNTKEGALKQLLREKGTYYICFKSLSKGYKTVSFDFDMDGVDKEYAQSEQFSEMSKELSRTNRNFQTIYRNQNWISDRENAHQMLLEQTQQNVKWCASAKIGILLIIAITQICVVYYFFKDKDFSGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T8G8</accession>
    <name>A0A8S1T8G8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">LITAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0180143</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDIP1/LITAF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T8G8"/>
    <dbReference type="OrthoDB" id="5599753at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006629">
        <property type="entry name" value="LITAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037519">
        <property type="entry name" value="LITAF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292:SF6">
        <property type="entry name" value="FI16602P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292">
        <property type="entry name" value="LIPOPOLYSACCHARIDE-INDUCED TUMOR NECROSIS FACTOR-ALPHA FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10601">
        <property type="entry name" value="zf-LITAF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00714">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51837">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="LITAF" evidence="7">
        <location>
            <begin position="30"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8148593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="117" mass="12905" checksum="A3FD941A4AA74FF0" modified="2022-10-12" version="1">MQTVQDNSSTIIAPVIDEAQITIGTDDRQSQSLIQVNHHANMKRVPITVVCPQCKQQGTTVIIREVGAATIMVGYLLFLLTTIFCFWIPCCVDECQDAIHQCPNCKAEVGIGPYQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T8Q3</accession>
    <name>A0A8S1T8Q3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Palmitoyltransferase</fullName>
            <ecNumber evidence="10">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0180188</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="10"/>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T8Q3"/>
    <dbReference type="OrthoDB" id="302728at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF43">
        <property type="entry name" value="PALMITOYLTRANSFERASE APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="222"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="245"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="12">
        <location>
            <begin position="107"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="331"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8148683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="418" mass="49673" checksum="92D243E5E6A2B9BB" modified="2022-10-12" version="1">MRVQHFENSLLIGQLWIGSVSDSFMIIFVLGSYAILQYYFLYFFSEDYYLTNSWISWTCLVMFLYSYLCGTFTEPGVIRRPQPDDEESFNSIKSNKSVRNEKVGEYFQERYCARCKIMRPPKSSHCYNCNNCVKMYDHHCTFMSNCIGQRNYRYFIGWIYTLLIQCIIWYFVLLQHLYQKFSISETIDKLYKSYYLQYAALTFLMSFCCNITFYLRGCCQSILNLLIIICCCICLYSGYNFDKKYYENYFCSLIFVIITLPGALMALSVGLNQSFHLAIGVNQKEWSVLERQININSIKDTQIELNENINPNQIIDTPDQEKSIEDQSICIEKESEEDKQLRQRIQEEIENNPELKGTLSQEKIEYIFQQRRNYHKKQKMIENANQQVQTYKNSASLKNYFINLKKVLFDKISQSELY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TAB9</accession>
    <name>A0A8S1TAB9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CDT1 Geminin-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0170006</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TAB9"/>
    <dbReference type="OrthoDB" id="341730at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032054">
        <property type="entry name" value="Cdt1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014939">
        <property type="entry name" value="CDT1_Gemini-bd-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16679">
        <property type="entry name" value="CDT1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01075">
        <property type="entry name" value="CDT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CDT1 Geminin-binding" evidence="1">
        <location>
            <begin position="107"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8147522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="384" mass="45111" checksum="8F0EC9A1702A2F81" modified="2022-10-12" version="1">METNYQKETKRPQQVIKMAKLNNNYKEPEDTYEPKRRVKNQIQQLNEDSSTFNGIEYFESLQSKQLSEKKEKTPLKLESVSSILAKGSLRERFSNLHLISSIDKMLMHSKYSSLCHFFKEIDEMLLFLQSQQKPPFLNTIQELLSQNSKIKVTQQIIQQILEIFPNAYDVNWSLNEKLIRLDRSDLIIKANIKSHVQLGERLNYFKQLMFDYIQKNGEQIGLAVLPQNPFFLNLQTKPETTDALTKQIQLQTQKHEIQSQVSKEPTYSKVSQNLIEKLKQKKKLEIVQTDHSAPKKQLIAISSLIHQYFLIRDVSNMFLTNIVKYSLNSLKQFILDENQLKTYIDQLIKLCPHWIQLIDNQNGQILRLNKEIDLPSIIRSIEQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TEU0</accession>
    <name>A0A8S1TEU0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0200245</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TEU0"/>
    <dbReference type="OrthoDB" id="309116at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="119"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8150408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="298" mass="36315" checksum="E51C22DA81D69597" modified="2022-10-12" version="1">MQYQKQLLKNTLEEQQQYRQQQQIDSKRRALEQEQQMLQEQAKALEYDEFQRKQLQKIKQRELSEAYEQSINQKKQNQNIIKQKQIQDEFNLVSQAQQYQEMQRQQKIQQQYQMKQIAQASMDDKMRKIMQQKEQQEQEKIKEQLNQRDEENRITDKQKQYRDYYRQVAEHQERMQKNFAEKIGSDKQFQIENMINRGIENVQYKEEMEAQQKKQSQLYNKQMIRDSLQKQIFEKEQSTKHQNGSQIMSNGFRSVENNQIDPSIHNPLLNPIPGYNQNPYLNQRSQLGTYGNQVLKMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TG77</accession>
    <name>A0A8S1TG77_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0210269</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TG77"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8151270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="151" mass="18197" checksum="33071E27AA95CAD7" modified="2022-10-12" version="1">MNQIIFDEDQIHNNTWDEYSNQLICESPILFGIPYRRKINEDQLSVIYLDYQSNTSENQQSNEKKYQTPHTLKRIQKKSKKFNAKFKTKQISLNNIIEEFTTFVHFSAESKNEQIHYCQKMRMMLENLESILFSIKQEILDQYQKKLSMNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TPZ9</accession>
    <name>A0A8S1TPZ9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0260131</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8154765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TPZ9"/>
    <dbReference type="OrthoDB" id="308589at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8154765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="90" mass="9940" checksum="337479C9413CE3A2" modified="2022-10-12" version="1">MNNDLCESIVNHANQIYISLANLNGQLNENGSMTNQNPTSSNTSPTLGLAFIMFTLFAAYALLSIFSPQRQPVMQKGNIQPQQNNLHDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TQQ8</accession>
    <name>A0A8S1TQQ8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0250322</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8154338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TQQ8"/>
    <dbReference type="OrthoDB" id="288132at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8154338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="265" mass="31369" checksum="F21326A60147759A" modified="2022-10-12" version="1">MSKSWQIHQSLQESLCSQLNKDEILEEFLQVCNHIANEDQNSIKSLNSKLQTIKKHYAQQKCRIDHQFKYIQTLLEQKRKELITQLNNHEMDLDKTFQNLRDEVTQIQSVVFNIQNDILSNRQEILSEVDEETFQNILTQFDQQIQMAQNYKKELLSTRITLIIIEEWKASHQDVIKHLLNQSITIKDYQKQIEPHHSELSASTCSDKMYSSNRSSQELPIKQTPLIISFDQKYYMQSKQTDITKSESDIHDSCESDFLEEWAKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U025</accession>
    <name>A0A8S1U025_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0310108</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U025"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14003">
        <property type="entry name" value="STKc_AMPK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000636">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF82">
        <property type="entry name" value="KP78A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="9"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="350"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="382"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="350"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="362"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8158000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="588" mass="67792" checksum="394FE84A73E37552" modified="2022-10-12" version="1">MQESTLGNYAIGNTLGEGTFGKVKMGTHLQTGEKVAIKILEKAKFEDESDVYRIAKEIEILKKLRHPHIIQIYEIIDTDKEIYLIMEYASGGELFEYIVKNHRVSEKIACRFLLQILSGVEYMHKIGIVHRDLKPENLLFDNNQNIKIVDFGLSNTYKPNELLKTACGSPCYAAPEMIQGMKYSGYLIDIWSCGIVLYAMLCGYLPFEDQNTNQLYKKIVAGDLVFPKWLSIEAKDLLKNILNTDPKKRFTIPQIKGHKWAKIIKIEEHFGNIGSDNIQVDEIIVEQLKNLYSIDPQLCRKLVKKNRHNNITTLYYLQIQKNKKNRTYNYFKKDFEDSITSSYILNSSNLDSLNTSNNNTSRQKQNTSNGHSPQHRIMTQINNRQQQSPSPQPMKQITKPDSPKQFNILLKRDIIKRPQIRPIIPPMLKPIYQAVNNSISSVGTNGNTPIQTNRKTEDSFSLDKAKPKVINRERAISAISEYTQPSPVKIPQPKIHKGAFNFQCTTHREPSIFAAELLKMITQMQIRILQQNDPYEMLCSANLSLKFEIQIRQIQNCDGLYLLKAHYISGEWEEYQKTLNKLIQLLNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U5A7</accession>
    <name>A0A8S1U5A7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0330036</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U5A7"/>
    <dbReference type="OrthoDB" id="77931at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="365"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="424"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="472"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="518"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="561"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="627"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="673"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="699"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="726"/>
            <end position="743"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8159253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="819" mass="95934" checksum="F3C57628C1A60C59" modified="2022-10-12" version="1">MQANLVEDYFYNQIILIIKLNPQQLLIMRHQMEEVFILISLQLYSYIILQIPFYFFNKAFKFRNNLIESPHHLELLINNMEMQSKKIVINQILNCKLILKPYKIIEQGISYFSDVLMIPSNQIIQEYQIYQPQSIQYFSYITEFGLILKNSLNEQLPNNYNTSCNFISKIIHGESQDQVGKQTEQTILKFDQEKDYYDLGSHSFILDPQNQQRLQIEINCQSSEKLRIFNYIIETKSFKCQLGEFYVNSGCQPCSSSQGFYSVVYDAKECSIFDQTKFKNITENNIELLEGYWRPNYLSDEIEECFKNLKFCKGGWGQGNQICDLGHVGALCEECDIYNIRGDGKYFQNQQDSQCISCFGVQDSIIPFIAASICSFISIIITLRSIEQSNQLFKCLKLSQRFNKIIFNLEQGHESFLIKMLLNYLWIFSSIFTFNIQFSFSIGFVDSVSNTSYSMTNNLDCYLSENQTIQLIYYKIITMLILMISQFILIIIGFLCYNWHKKIGLSNFNLEIISNTLLYLYISNYGGLVKMYFSILSKRDVSSQSYIQGDVSLLFESKEHLIWIFSFVIPGIGIFSLMIPLSLFIVMYIKKGQHDKINIRKHFCYLINEYNVSSYFWEDIKLIKKTIIILILTYFETYILLKASLLGLCLLFYQLIAFNKKPYILSNFNSKDLFSALICSISIFLVAAKYVAEQENNQFSSISLQTIIVLLCIKICYSFIKSILEVYFNKYFILILNYLYIILNKISSNSIFTKKLQNNLQKSTQKNKRIKILINKLRQHLLEVSKSQLQNQRKILSSKIKKTKSWVSKQGYFLKIDTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U5L4</accession>
    <name>A0A8S1U5L4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0340049</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8159898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U5L4"/>
    <dbReference type="OrthoDB" id="286017at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14137">
        <property type="entry name" value="STKc_GSK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000082">
        <property type="entry name" value="Shaggy-related protein kinase kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050591">
        <property type="entry name" value="GSK-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039192">
        <property type="entry name" value="STKc_GSK3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057">
        <property type="entry name" value="GLYCOGEN SYNTHASE KINASE-3 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057:SF0">
        <property type="entry name" value="PROTEIN KINASE SHAGGY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="117"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="7"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8159898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="458" mass="53305" checksum="7BC41AA6BCAD50AD" modified="2022-10-12" version="1">MFQKMLNQQLKKQSEEQNRRVVTEVEEMDKEIKTLQDISNLKARLKMNQMVKQPFKKNKEEPTTSIYVGKFENQLKSFQQQTIKKQSRKPLSIEKNSTKSVLMPATTERYDYSNFTYTNLKVVGSGSFGVVYKAKVNETGEIVAIKKVLQDRRYKNRELQILQELDHQNVLKMKYAFYTPAENKDESYLNVVMDYFSDTLYSYNKSFIKDFKRMPELLVKIFSYQLLRSIAYISILGICHRDIKPHNVLVNPETNKLQLCDFGSAKRLILGEPNIAYICSRCYRAPELIFGATDYNTQIDVWSVGCVIAELINGEPLFLGDSAVDQMVEIVKVLGTPSKEQILSMNKNYDMQQYQFATIKQRDWRRVLKTKDPKAIDLVSKLLTYCPKTRFTPLQSLAHPYFDELRDQFKTIQSTIKLSASDLFEFSNEETSKMTQQQMITLIPDWYANTSKPLKTVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UAR6</accession>
    <name>A0A8S1UAR6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HECT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0350184</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UAR6"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000009">
        <property type="entry name" value="Probable E3 ubiquitin-protein ligase HECTD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000024">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF440">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NEDD-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="4">
        <location>
            <begin position="142"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="2">
        <location>
            <position position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8160812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="468" mass="55098" checksum="C69AF06A4B855FD5" modified="2022-10-12" version="1">MGNRSSNNSNQKIGQEAKCPNCDRKFGPSTTYNLLNTHIDHCLQNQQQINNIGFQMPQPQVQLGDNYIWVKQNNQWKRVQSQVNGGQIQNLSVQEIKNRSFPEKQMWFNLQLEKFRIPWQLGSDKLNVNLNDLLQSSLISARNVNLYKEVKVVFQNDKVQDAGGLLREWLTLIFKEMCKDIFSLTETNDVTYKIAKQSQYFDLVGLAIAKALFERMNICVEFDRPLVKRLIGQEISFQDMQFYDKSLYLSWKYLLENQFDENSLQQYFIICKDDEIIELKQNGADILVTNQNKQEFVDLSIQFYSEKMISKQLGQIQNGLYKYIPKDYLNIFTAEEFEMILYGVSVVDLDEWKQHTTYKTPYADTSQQIKWFWKILSEFDQDQLKKFLHYCTGSYRIPVNGFSKLESNRGMYSKFQIIPIDYINANSFPIAHTCFNRLELPKYSSEEMMRKYLRSIVLNDLEGVFGME</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UC30</accession>
    <name>A0A8S1UC30_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0360072</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UC30"/>
    <dbReference type="OrthoDB" id="289935at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035898376" evidence="2">
        <location>
            <begin position="17"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="58"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="261"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8161242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="368" mass="41497" checksum="CD6A0C330EE6D069" modified="2022-10-12" version="1">MLKLALCVLLVLGATAINVQSSIWTNRDQKALAQIHSSGWGKFILNYAELHMSTGGILSELNSEIEKLVGELEEELAGVHHEFNRRTDVHNREVSRLEQEIQDKERELFNAHDFYDNVLIPQRDRFAAQLEQLQENIAHNRQTLEQATVQRENDHETFEAEVAEHNDAISAIDECLQLLSTITAPSLAQIKKVQKNLTKIQNSLKKHNQFQIFVKVLLEITVESNFADQGALRDIVVAFNNLRVELVDSLNQITADEAQAVADFNAQVAQLNQEHAEFQRAVVVKNAEIEANATKIEQTLDLIDELDADLATLNGQLQAENDDYAFATDVYNSTVAEYNKEINAANQALELLNQPRFQDYVKSQLKGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UKW5</accession>
    <name>A0A8S1UKW5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0430142</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8165818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UKW5"/>
    <dbReference type="OrthoDB" id="424834at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000576">
        <property type="entry name" value="LacY/RafB_perm_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052187">
        <property type="entry name" value="MFSD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23512">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23512:SF3">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01306">
        <property type="entry name" value="LacY_symp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="156"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="281"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="307"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="347"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="372"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="408"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="30"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8165818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="466" mass="51723" checksum="B750EBA54EE6CDA1" modified="2022-10-12" version="1">MNDYEKKDQSQELITNKQKSWHNTNVRWIALILACLFQFGSYFSTDYPSVLAEEIKDSFGKNQSDVNLLYTYYSLPNVILPLLGGLFIDAIGIRLAMVGFFAILIVGQFLCYISAVTGNFTTMLIGRLVFGLGGENCVVAQSYIVSKWFFGSELAFALGLNITFARLGSVLGAILLGKTYIWFGNSLSASMFFCLALLILAWVDAIVLALLDKYCDKRDNVEAKIEGDKITLSDIKEFKLDFHLLTLSCLTCYSAFFLFQYNNVEMFKLIYHMSPNQASNVYSIPYYSAALLTPVFGFVIDKFGRRTHLLVLCGGLLVLVNAMFITITCDTSDTCLTGPILGQILNGIYYSLYAAVMWPCVPLCVPERAVGTAFGVVNAVQNIGLTVFPIIVGHLISDETPTAYRHMLLFLGSTAIIGLISNIGLWFVDKQNGGKMAKPSLKDNTEVQDLSIQQQIDYSISEPKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ULK2</accession>
    <name>A0A8S1ULK2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GB1/RHD3-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0390209</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="312156at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01851">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001470">
        <property type="entry name" value="Interferon-induced guanylate-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030386">
        <property type="entry name" value="G_GB1_RHD3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003191">
        <property type="entry name" value="Guanylate-bd/ATL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015894">
        <property type="entry name" value="Guanylate-bd_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10751">
        <property type="entry name" value="GUANYLATE BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02263">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02841">
        <property type="entry name" value="GBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51715">
        <property type="entry name" value="G_GB1_RHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GB1/RHD3-type G" evidence="5">
        <location>
            <begin position="36"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="486"/>
            <end position="1533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8163416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1636" mass="194182" checksum="6F70554CF0E799DE" modified="2022-10-12" version="1">MKKSFREEAMPLIFYQQETQTYQVNPEAIDIIKNIPAPIGIVGVAGMYRTGKSYLLNRMLLNRSDGFGVGPTVLPCTKGLWMWGKPLLGQTSDGESCSILVVDSEGLGAPDEDSTHDIRIFSLTILLTSCFIYNSVGSIDENALQNLSLVVNLTKNIQLKNGQSTTDLEDLSQYFPQFYWVVRDFTLQLVDRNNEQITSKDYLENALTLQKGTSDGIDQKNKIRKLLSTFFKDRDCITLVRPLTKESSLQNLENLDFDKLRPEFFEQVINLRKKILNRIRPKTLNGKTLSGQMYCDLVKSYVTAINNGAVPAIESAWTYICKNECQKAVAEAFETYEQILKENLHNRFPISNEDLKAFNRSLKEQAFALFKKKCVGEYDEFKNELSKRIKNRFAAVKQENDREGSRMCSQFIQQEFQPIDRKLKLGEYRTFGEYEKDIKMFYNFFIENGPRVGTRNQIILEFLQRALIEGSNLFIRQYSQETEMMKNVSQETQKKLEQELKEARQDGLKDKNNLLMKLAQIESEKTEIELREQVARDNLEELKIQKDQVERDLKLESENEKNELMRQIQELKGQVLKAEEFNKDLERTTLFGNSEFEKERALLEQKITFFEKLVNEMNAKEIDYQNEIKNLRKEYSLQSKDQQSKADQSVRQLQQKLNDLQEKLNEMENELIEKETNFENDFKKFEHKERSLTKQNLELNEQIQVLTRELREYRRNEEQVQLMLKNDANNQVNEMIEKVQNLEDLIKSKDDQLKQTRSQSEKDKALMQQKMEFMEVQLDEYKKQIEENKKSHEAIMKAFENSSNESTYKIDAAKMNELREQHKRELKNIENEYENTKKRLQQQIDQLNEKNNELELKVKFETGDLIKELDNLKEQLQVTEEQRNKLLDQNKMLDGQKLQILKEQEIRYQKKIKQLEQAIDESDAKIAKEVNLAQAKAEESLTQLKNFYEIERERLERRICEEKEKSEKKFQAAQEEFYHKLRETEQNYEEEIENLKDDLRDQVQQYTNTIQQYDHEIALKQQTIEIFEKHIKETKEQLISLQNNNNATLEQQMNSFTNERKSLIQKIDVLSSQLNNLQKEHMALQQKKDLLENEKARKEQQFEQSRKEWHEEKKEQLERLEETKLRLQKMNDEFLEKKIEYGRETALIQQQNEFLQKKIEDLQRQIDTQQSRFDEKLKQQKNEYLVELEQKLERAQEEKTTIETKYEKIKKQLKEAEYQYNKQASTLEREKAILTEKLGHLEIRKNELESKLKDEAATIAQQQASLREQIAQEKKQLQQELEKYKQLNLQLEQDKSEIHTSYERDKALWEGKFQFLEQQKEQAKQDLVDALKKFEMTLMHLQRARSNEKDEQENNLNELLLSVERKYQAQIEEANQTHQRIVQDYEDKIRRLQKEIKTHKDKILIDQHGKIGNQLLSEKKFAEMLDNEKRLQQEIENIKQDRDQKILEYQRMLEQERESLKVKITELETKYKEVESKRSTLIFEFEKERAKWNLDRDHLNNLKNELTDQLDRLRKKEELLLRDNEKLKNEQRATRRSVAAHNMTSNNIMTGNKYRNPINNVSTIGLQKLSPTHSNNTSTSSANISVLKKTNLADITNFEKVVPPTQQSFQNNKYYFYGNQQGQNDDSMIGQSDFQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ULL1</accession>
    <name>A0A8S1ULL1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS17 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0390214</name>
        <name evidence="6" type="ORF">PPENT_87.1.T1270031</name>
        <name evidence="7" type="ORF">PPENT_87.1.T1370116</name>
    </gene>
    <organism evidence="5 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000127">
        <property type="protein sequence ID" value="CAD8201244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000137">
        <property type="protein sequence ID" value="CAD8204510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ULL1"/>
    <dbReference type="OrthoDB" id="299793at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00364">
        <property type="entry name" value="Ribosomal_uS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.1000:FF:000001">
        <property type="entry name" value="40S ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_uS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032440">
        <property type="entry name" value="Ribosomal_uS17_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744:SF9">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16205">
        <property type="entry name" value="Ribosomal_S17_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS17 N-terminal" evidence="4">
        <location>
            <begin position="5"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8163426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8201244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8204510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="159" mass="18231" checksum="8F996C74604FC776" modified="2022-10-12" version="1">MADLQTQRAFQKQEGIFQNSKKLLAKKTSKGVRYWKEVGLGFKVPKEAIEGHYIDKKCPFTGNVSVRGAILKGIVISTKMTRTIIIRRDYLHYVAKYNRYEKRHRNVPVHISPAFGPVKEGDIVVCGQCRPLSKTVRFNVLKVIPNEIIGNVRKQFVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UZ01</accession>
    <name>A0A8S1UZ01_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0510068</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000051">
        <property type="protein sequence ID" value="CAD8169477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UZ01"/>
    <dbReference type="OrthoDB" id="76105at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19756">
        <property type="entry name" value="Bbox2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="6"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="8">
        <location>
            <begin position="97"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="390"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="187"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8169477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="575" mass="66385" checksum="B642DC7A6C9964A7" modified="2022-10-12" version="1">MEELICPQCHNIFNEFDNTPLMLPDCGHTICQQCIQSMLSNADGQQISCPEDNILAKGKTNILEFPKNCQLLKMILKQRPSIEQPEYQLNLNNFAKEQFELCGEHLEKLEIVCLTDKIRICTKCALFGNHRHHEVKSVDDVVREVTLKAENIMQTYQKILDKQQEMTESKFYEHLSDKFSIMFQDSQMSVKEKFKELHQQLEQKENKLLEQLNTLTQTLEQQTKKQIKDQIQQSLQQAELWKIGAKDRLLYFSTKTDNGELPLDLLYNQEFKGNAIIEEMDRTIKILEQKINNIRIRKIRVDFKKTEIEKCFDTMCSLTLQLNHTENNPVENSFTKFDQEEPLLLRDNSVADWTDTDESLITSITQNQSHISTQSVIQAKSPLRTQNLTQSHHSITPLKNIKQKSPNKEQSNNTLQPPGPISILRQGISPTPKLQERRKKTFKMNEKFDSIWQAFKNDNLEIVDFSNAELGDEGCQIIADQLKICKKVKQLKLARNKISDDGATVLLQALAQNGNVVSLHLSSNMITERTLDQILQLIKSSQLPKNIYLSQTLINATKSKKKVEELKKLGYIVNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V1M4</accession>
    <name>A0A8S1V1M4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0540219</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8171288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V1M4"/>
    <dbReference type="OrthoDB" id="10009520at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20336">
        <property type="entry name" value="Rcat_RBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="206"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="235"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="287"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="321"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="1"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8171288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="342" mass="40628" checksum="F2196814DC3A976F" modified="2022-10-12" version="1">MEDICIICLQNTQNFVIGKSCQCKYCYNCVFDWFEQNPEKLLKQSFNCLNYLCKEPNSIQEFLNSINEQKILDRYNDITFKNYLKSCQDIRACPNKTCKSYGFLPSSRCQEFLECEICNYKWYDKKCLSINKICMLFLKNLKNDFLCFFSEFYYTHQCPNCDTQIMKNGGCNHMTCKSCNHEYCYICRDKHNGHDSKLCSLKDNSFCIVIGYVIIQLLFTLGIHTLLIYIAKQLFQLVCIIFIFAYDLNGKLITICTMSTLLDMKYHYDIHRKESVKLYRTQTEKKINWKIFIELIALLVLDLIVILTTIYEDNINKIDSIAIYVIYPVEFLLMLFICFWYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V8B6</accession>
    <name>A0A8S1V8B6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Inosine/uridine-preferring nucleoside hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0580100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000058">
        <property type="protein sequence ID" value="CAD8172871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V8B6"/>
    <dbReference type="OrthoDB" id="432381at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016799">
        <property type="term" value="F:hydrolase activity, hydrolyzing N-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001910">
        <property type="entry name" value="Inosine/uridine_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052775">
        <property type="entry name" value="IUN_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46190:SF1">
        <property type="entry name" value="SI:CH211-201H21.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46190">
        <property type="entry name" value="SI:CH211-201H21.5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01156">
        <property type="entry name" value="IU_nuc_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inosine/uridine-preferring nucleoside hydrolase" evidence="1">
        <location>
            <begin position="26"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8172871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="340" mass="39027" checksum="5CFFBD35FC73D94F" modified="2022-10-12" version="1">MTNNFKIPDMLLGNDINQYKKYTKFIIDTDAGGDDAHAIMMASYILKYIRKDAELLGITCVSGNASLYNVIKNVYITSRYCHFDEFPKIYKGCEFDSMRKFHRDFFFKEDGFGGVQNQYLKELNVNENQQLYENQHAVDFLKESIEKYQEELCIICIGPLTNIYITLLQHPQIINQLGCLFCMCGTYMGIGNQLNGIAEFNIATDVLATKMVLQADFKNKIILPFDTVLSYSLNKDVGSKIFNVQYNKIAKLIHDIYENVVFETCYYLCDELAIVMAMMPETITQALLRGIHIVEDGFARGGMIVDWIKKMKKEDTSQVAIVTQFNWDQMVGLALKTNQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VB92</accession>
    <name>A0A8S1VB92_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0600090</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000060">
        <property type="protein sequence ID" value="CAD8173851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VB92"/>
    <dbReference type="OrthoDB" id="303946at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="356"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="165"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="356"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8173851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="379" mass="44091" checksum="350D7DD553B2BA44" modified="2022-10-12" version="1">MSDFQSRQYVTQSSQRQYTSGTKDNVRSITQDRVEQVITRETPKVQTTTVEVEQRSATKIPRMQSQQILRTQTQGFVGGQSYISNMAAIPQVGYQGNYSVQQNCVAAQSLCMNVIVVSKEEIEAPWRLECEYLQSLIAELEKRKEVQVVEKVVEKEKVVVDNSKVDFLEAQLRQLRSENDQLQMQLQSMRGEIQLRSSQTEDLASKEREFFNLRIRFESQIKDLEDQIRRLQEENGKLRKENNELLIKLRDYETKIAMLSSEIERLVYSNKIKDGDLEDWRLRYEQLESQGQTVIQEKVTYLSSEVEVWKQKFIKVNHDYNECQEQLTMCQAELEALRKSQKKEVVVSTSKVVTRTGGTTTTSSMGQTRGSRTYEQFQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VCI5</accession>
    <name>A0A8S1VCI5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">USP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0600037</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000060">
        <property type="protein sequence ID" value="CAD8173749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="289038at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000136">
        <property type="entry name" value="Ubiquitin C-terminal hydrolase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056850">
        <property type="entry name" value="ARM_UBP34_24_USP9X_Y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF827">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25010">
        <property type="entry name" value="ARM_UBP24_USP9X-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="5">
        <location>
            <begin position="1416"/>
            <end position="1752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="2348"/>
            <end position="2375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="2362"/>
            <end position="2375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8173749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2375" mass="283287" checksum="DA55C10DE70ADC76" modified="2022-10-12" version="1">MDVKKSKKKPELNEQLFVGNYIDVFHQGSKQHKLAYILQKSEKEIEITYDGLSRKENEIIKLSQNRINFARRFTLNYTGDDYRQSKTSRDYLKYSKEECEKNIKELNSIMQNNFQGLSPIEICQSVRVRQFIWLDMVLSSEFQSKELPLALEYIKTYYNFIKWYFEQFPKYFADYMRFQNNQELYILDERVSIAACLQEVCEAFCMLFGSIWRLLKQENSFFYLNYDNLQQYFEKFFPTQNFQNLTLNPNIDDWNLYQGASDIQKCIRKTWPFYLRTMLYFRQIGGLQAWEGLLKPQDNTEYNYIPLKAMNRIVLTQKYLSQYFQQSDQAQMAKRTFEWLQNRVNNMTIQDIKDTDIDQVKDLTTDHQYYFQKGFTEDQLNRLTDEIQLQLSLKFLKSSFLEKRVKGISEIKDFTEKLKFDQQTNLKFRSSINKDDLIKWISQNKILDYTLLGDSVHPELIKRSSDVAVFLCRNQAFQIDYIDKIWINNYDKHETTQLALYEFFKIVSPYLNFQGIEKLYNHISLISYSKYNENIVSMIKTFTESALSQKFHEQQLQLNPEKRFMTFNQLWELLQDRDDQLTNSVIQEQCFSAARQIISQVQQTKQFISCYFGKCFELIGSHQSVYQAISFVHYFLDKQFKDDFNGKRELIQSTDDKYNIIELFVQDIEVYMEKVRQYFKNEIPQDIIINGVQKFSQNVFFRLQMLHYLLQQTHLKITYEQSVRLWDTLSTKTKGGIQKKELNKIFITNYQMDTNSFRICPIYFDKEGESKFFKQILCNPERNDYENYTIEDFELFQVFFKQYNQTRQTLKYFNNLYRFLVNDHNFEGKSAIWQIFAKVKDQLLLEQISYFIINLYTQLNQNLDQQSDKIYQEMMDKCLDLIQQQCPSLTTRSINLLLQLFNYFQSGPPSKKSSKPINYTTFKVQLQQPNNLIKYMEFKEGDNTTINQWKQKLAEELQVAYLQLDITIENKPIEQQFDVVETFVSQAFYQLATVKVKINNKNHPKNYLSQEQRTFEILFKLLDKQESIEFLSQVWDLINRLPINIIKKRQVENCKDWKQYLDHQFFDMFYVLQIIQALLENEQWCEQFNNSDGVDLITQKFLGQPFQFQQRPLEIKCCLTYLEILSHPRIRIKELYIIEQIKSKIIESIQQLCHFIKNKKKLEVGQRKSPQQKEMNDVESRLLRKCFLYLDQDGCRLYIKNSELNQQLYTFFVEHENHELKKEYSLQLLNLRNTSESRLLIKIVLEDVLKNVIQNNKYKCDHFFEFCCNLIQQEQNLAIQKLNFEELLVYIRQKLEELPFNENTVKDQDQILIGLLKLLNVLIDRIQQLSNHQGLFEQILSYLFEKEGETRCKCKSQQSRTAGFNCFFTLLRSPQNMQKFLNEVSHLHYTHTWRTKNFNDWNIQSKFHEKSSTGYVGLYNLGCICYMNSLLQQLYMVPAFREKLLQIEDKSTCAQEENLIHQLKCLFLALKHSQKQYHNPKKFCHAFKDFDGNPTNIFEQMDVDEFCNLLMDRIELNIKSTTDEDLVKRNFGGVISNEIIGKTCPHYSEREEPFFAISLPVANKKNLEECLQTLVQGDLLEGENAYSCEQCNKKVSALKRTCIKKLPDHLILVLKRFNFDFDLMAKAKINERIEFPFELDLLPYSQQGLRQQENRANPQNGQDNPAEYYQYRLTGVVIHIGSADSGHYYSFIQDRCDFNKWYEFNDIFVSPANIIDVKNDGFGGVDRLLKTKYPNQFKDKSKSAYMLFYERIQPLNGKEELMDIEFDQKTSQFLDEIKVENRKFQIQRFIFSPEYFIFIQNLIKFELQSNQVSEQIVQTLVFFYLTCAVRENDKTFIQNNILDIQELLKRAPNTCEWLLKCFNQYHYIREFQFDCSKKMVRKFVISLIVTAIDTVQKKEGYKILYEYVDDKPKSLVASLINSWIRLIPDLKRSLKNSIEYYDLFYRFAKLNQQNSQYLISKKIVGKFLDLFMDTMQINFSKLLIPSKESVRKLDDLKVKQFTEDPNSFLGYQNIQNVDIASNYYDELLEKKFEKSMNSGPSTSRVYMWRLIAFLLKIEGKNALSLEEQNLLQFDNPILMGILEEGDCKLAIRMISDILSILSQDNQKQTEQIIQAIIKQINDKEYKEYRKYLVVLKRLFTLKDSLQQMRIHQGMSKLLEVMQKQSQYFFETDVCQQYILRIIFRNQAVYQWMIKNQRLWQWIIETNNTQSNPNEKLISNNSNSQKCIHRLHNIYLPITSQAYVKFLNWKKLQFQNLAKEPFKQNEDFDSDDDLTDKIVKVDDKIDYYDQNQWITATISKIMGDYVHLTFSGKMAPQNIDIELDCERLAPFNTLSSNNKIPGYNNQEMAIEHQSDHDNDTEEGNNNSINQTDSDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VEA2</accession>
    <name>A0A8S1VEA2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">H-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0620058</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8174911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VEA2"/>
    <dbReference type="OrthoDB" id="286359at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019019">
        <property type="entry name" value="H-type_lectin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09458">
        <property type="entry name" value="H_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="H-type lectin" evidence="1">
        <location>
            <begin position="38"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8174911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="372" mass="43078" checksum="7410385D9626C290" modified="2022-10-12" version="1">MYVLLLQLVILIQGYPILENGQLLIGAGGLACEKDTITHIKFLNVYKAYVPQVIFVLSNYTNEMQFSDFALDIHVELMKVNTTGFTIQVVCSKSCIKSVLYTWYAFGGDTYDSGCFPLQERAQVTLEQIYTSPMQHSVFISGFAGFFKYGGKKPMLKLYSSFDNQNLTIQVESTFQYVYICYILAQDMSSTTLNDTEVATLVQKGTYLDDTLIMGMESFQLFGNNLQIQMLNDKSFFPKQIELTVKVAKLYEPTYRSCPKVYTECMFQGDPIYVCDETVKLTNIWKEFKSLRVYDQKMKFKLNIVSNDNILWLSEDIDCSYDMYDQLMEFKIKNQPIERQIIRKRKMMGMLYDEQTEEDQEYSEDTILIGQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VF07</accession>
    <name>A0A8S1VF07_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAD synthase</fullName>
            <ecNumber evidence="2">2.7.7.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0620118</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>FMN + ATP + H(+) = FAD + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17237"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.7.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.2" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8175023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VF07"/>
    <dbReference type="OrthoDB" id="270728at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003919">
        <property type="term" value="F:FMN adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006747">
        <property type="term" value="P:FAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23948">
        <property type="entry name" value="FAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23293:SF9">
        <property type="entry name" value="FAD SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23293">
        <property type="entry name" value="FAD SYNTHETASE-RELATED FMN ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoadenosine phosphosulphate reductase" evidence="11">
        <location>
            <begin position="99"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8175023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="205" mass="24190" checksum="2A3BB67C99268A33" modified="2022-10-12" version="1">MDNQNSQSTTILFEEAIDFLVKIFKIFRFPQIKICFNGGKDATVVLYLAKMALEKLQISTQIECLYFKEQEPFPEIIEFIESQKKVLNLQVLECNECVKQSLEKQEQLQAVIMGTRRSDPHGKNLNLISITDNNYPSLLRINPILEWNYSQIWEFIRTYNIPYCSLYDQGYMCLGEIGKTQPNKNYKKGMKPWEFDPQHERLDRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VHV8</accession>
    <name>A0A8S1VHV8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0640180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8176123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="311989at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14014">
        <property type="entry name" value="STKc_PknB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="19"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="478"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8176123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="750" mass="89008" checksum="76BEE8779E95175B" modified="2022-10-12" version="1" fragment="single">MRMNQIIQSPFNEFKDRCFVILQCLGQGIEGEVYEARVENQSYYEKNVAIKFYTNINQREEQFIDSILEQKQNNLHEYNQFLVQIYEKIQLNNEILVVMELGQCSLKEYLSRNELSFDEKNQICHQISKSIQFLHNQNYFHRDIKPENFVKINKQFKLTDFGLIKKSFESERIPTICGTQQYQAPEIQDEIYSQEIDIWSLACVFYEIIQGEELIQGYKEKQVKYNKKDLLNNIEDNKQIQKMKQSQIPQWSDLIIKMLNQNPKYRPNILQVINEIEKIQQINIEDNIIRNPQKEQIQLQEYLNQNNNLSQQEKESIILDISKSILHLHKNGYYHTNIDLNMFQKYNNHWVLAEFDSNKRDQSFNNSELDPISIDIFRLGCLFYYILQGKIVSDIRKVKVNQNLQIKSDIDSYTTDKDTFLTKIQQIKSEKWQKLILHMLESIEDTSLSLQEIIQVCISQNVNSLIDAPTQEFPKRYFILTKRFGYGGEGSVYQATPKNEKFYNKDIAFKIQQKMKDHEIQFIDFLIDYQKNHEQNQFIKSNLIKVYERFESQGQQVLIMELGGKDLFSVLKNKLQIEQKIKICQEISQSIAFLHKQQLIHRDIKPENFIQVGTTFKLIDFGLIKKKEENVRLTKMIGSPLYQAPEIIKGSDNYQASVDIWSLGCLFFELFQGEPLFDGQTLQEVYQQILNYCKNQQQIHLKINQLQIKQELKTLIKQMIDPIPDKRPNIDYVQKELLPKFLPYPIKNIX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VJQ8</accession>
    <name>A0A8S1VJQ8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0670076</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000067">
        <property type="protein sequence ID" value="CAD8177207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VJQ8"/>
    <dbReference type="OrthoDB" id="321504at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8177207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="570" mass="67982" checksum="BB8F20EB45A5E0CD" modified="2022-10-12" version="1">MIQDEITICFMDGEKRQCSKKYDDIMEELKKKEDQTWFLIEEQLKVLIKDDTTLKYSSQLISVGPSDIISMYRSHLKVKELETKNSQLEDEIINLQDAKSELLHYGKVIPQKYIKEGLLDQELEKQRKEIAKLNKCVQELENQLKSQEEAEQKRISDFKNKYQQLTNQESTYTQQISSLEQEKFQFSEKIDQLLQEKKQFSQKIDQLEQEKEQQTVQIAQLKSQSQPFQKEYQIGTEQSLEIQCSSIRIQNQGSKQSPTSINPQTVQHLNNIYSDERKQSESYIYLYNQNKFLESLLEKVCDENRELRKIFDPNKTILFQRQSKIYQIKLDVQQFSQSIKEKKFYFRKNCLFKDNEYLIELMQNKLDTEIKINIDDQCLKIKNVYHICQKKYQALDIILNMLIAEIYAQLFVRDLQEANKTNSQNQLSQLWNFKIPRLFLAKDNSGQYSIFQECFENDSKDFKPFQSTQIEDQYISSFFKYFYLATNKNFAITECQLLVLENNIQRRLNEVVIQSIVIHSKYKNLFSQLDKGKEKIIDFERILSQNPPGAIQQYWSEQLQILAEKVWYDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VJZ1</accession>
    <name>A0A8S1VJZ1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0620111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8175016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VJZ1"/>
    <dbReference type="OrthoDB" id="299243at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18773">
        <property type="entry name" value="PDC1_HK_sensor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="355"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="398"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8175016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="564" mass="67120" checksum="E4CD036CE665E279" modified="2022-10-12" version="1">MQKLTRFNSFIHHFKCIRNMKMQTQTFILQSSIFVLVFLIIITSQMINKNMMDDIIQRITKEIEIKTNLKQINIQSQFIKYQAHYPLEQGFKFLESFNRLNQMILKDQQTSINKLLDCKQVEESFNFFSKMPLFCYKFTKTNIITSDESLINHIIGYQALLNSIFLPISISQFQKTMFFTFISDEEYFAILPPKIVPDIYLPSQRPWYLEQIQSIQQTKQNDVIVISSIYQDFETQKYEFTLTKGITDQEQHFRGIFGIDQTLEHYKELLLYKDLNIILVDLKGRMLLSNLMIEQEIDLSKEKKYIYEIGTTGFDENDWKQIVNEANQQGQGTIECSDKDIKQFCRYNTFFKQDLIIIVINISSQFYLLVFQSLAHITTDVTRAQELQDEIMSEITQFLYYCIIVAVILLLLSIIIIPIFFRPLKNIQTMIKTQMVQKFTIHYDHLFFSNRKKNKLAFFEESFNVFKQKLLDFTQSKSQDCYIIENFKYPRQQQIIISELTSFIKFQTFKDKIENLPNQNNQIVQSPKLFCDTFLNYSQDNQNNVIQISPRDVTFRIELQHNQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VN90</accession>
    <name>A0A8S1VN90_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0700037</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000070">
        <property type="protein sequence ID" value="CAD8178650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VN90"/>
    <dbReference type="OrthoDB" id="304408at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="325"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="8"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="92"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="325"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8178650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="376" mass="44536" checksum="E61CE64AC24CDEDF" modified="2022-10-12" version="1">MDQTPTSINTFTTTDKELENNLKDLLERILVYVEKYIETKEELNQIENELKDYESLSYLVGIIKVIFTKLMLKIEKKLLKLDKCNDLNKSYMFKDDEEYDKLEQTLIKYEQEIRNHISVEQQLKLYAESIQSKLDESEQIRQELLETTKNNISKLKRENQELNEKEQQLQQEVNNLKQTISNLEKENKRKSIDSNQRDYLQALINKQSNQSSNQQKNGPNQKFNLDQRQLNSHSEHKSNPKQFLKEQQELILPTQSSQKLNYYSIITNANNKQKSEVIKSMQQKDFNNIYSQIMRTKHNSLSSINDLIQNASQQDKKRLDQFQSISKNSSQNNSMIQKNRESKQIEPLELQQRSKSSKRAEDIIKYQTLESLIKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VR07</accession>
    <name>A0A8S1VR07_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0720046</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000072">
        <property type="protein sequence ID" value="CAD8179447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VR07"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002896">
        <property type="entry name" value="ras-related protein RABA2a-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="182"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="191"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8179447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="227" mass="25632" checksum="4B4CDDE5E9D84304" modified="2022-10-12" version="1">MDQSQGVNDIAHVFKIILLGSTAVGKSQILLRFTKDQFNLSSNTTIGVEFSAKVLEIDNKKIRVQVWDTAGQEKYRAIAKAYYKGAVGAFLVYDITKKNTFLEVDRWLQEIREYSDQQNLVLMLIGNKNDLEANRQVSKEEAIKYAQSKKMGFLETSAQTGHNIEFAFKQIAEEILRDISETQDDDEFEQTKAQYSKNKGQTSNASVLKAADHQVKKKNNNSSGTCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VS08</accession>
    <name>A0A8S1VS08_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0720074</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000072">
        <property type="protein sequence ID" value="CAD8179501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VS08"/>
    <dbReference type="OrthoDB" id="308468at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052667">
        <property type="entry name" value="E3_ubiquitin-ligase_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47156:SF10">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM-21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47156">
        <property type="entry name" value="PROTEIN CBG20824"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="39"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="235"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8179501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="263" mass="30914" checksum="B090D2FB47289D29" modified="2022-10-12" version="1">MKVVQNPTQYFFEQSNNPPTEYLSKINEFVSKLLNQRQCILCKNDYNMKDRLPRILIHCGHTICTACLTNFYRNRRVRCPLCLKLIKHLDSVDRLPINHTIFTRMADDINNKSKQHGGTEVIDPQQYLFTQFQQSALQSQKARQQQQNAYPQVDPDSGLEFCEFHNDRVKHFFCMKHKVTCCRVCSEMIHQKKDCIVVDLYEIEDVPAFLKEAYKLNEDNTQQQNQRREDMGFIQDGEANDGENQFNDDDLEDEFEGNSIQSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VTF3</accession>
    <name>A0A8S1VTF3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0710061</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000071">
        <property type="protein sequence ID" value="CAD8179069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VTF3"/>
    <dbReference type="OrthoDB" id="292510at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8179069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="345" mass="40954" checksum="453C1E6AAB5FCF76" modified="2022-10-12" version="1">MNQKEYVFLERNNEYVENEYFVGQKESRFHSNDQQINIQVESQLSTLSYIFDWITNDQLQELRSQILHQIKLLDQNVKTKYITTALSYILIKNTSGINETECIQQLLNKFNVKSRKLLKYLRMIKDDGQRDMNKLLQQIQYYQEKVRKYFFGQGQSLLLKLKKPNQTYTQLIEEILETNKQVMIRIFSLQLVKQFCVSKRPSRLVINLGYFAFQIQSIYILPGEYARIFRISINSLRHFSKGLINILYQFVLRITGQGIETQFKSCERSPQISQTIMNEGELKKSPEDTSVSEPNIEIEGFKLLIIRKLINEFSIYQFLFVDTEEEDELFNECQEILNFSINDKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VZK0</accession>
    <name>A0A8S1VZK0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0790106</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000079">
        <property type="protein sequence ID" value="CAD8182750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VZK0"/>
    <dbReference type="OrthoDB" id="286063at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00454">
        <property type="entry name" value="TrHb1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001486">
        <property type="entry name" value="Hemoglobin_trunc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01152">
        <property type="entry name" value="Bac_globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8182750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="632" mass="74199" checksum="CAABFC59FEA897CB" modified="2022-10-12" version="1">MNRQFQQNCPFRQQYAGQYDFLTKSENTLCKGKVKLIQQSPSFADYVNALKDQGQKKNHRHNTETIQKCPILPKIEVVLPDLQTMKSHFVLSLNKSTTHSYHSQSYIQHSNSQTPRSRIGIPTSITRRSLPPQKQTKLSTITPKSSLCIKSNFDEDDGIFLTKIGGEEVIQEIARLFHQHSQIHPIIQKIDDPAMYESKFGTFLEYIMGKPVFFNIEALKQKHIPLKIDHALYNQFKSYLISSFIQSNKGPPELIFEFSALIEQYKYCIITSEQTFAQIYNQRTENNKDETPQSIVHLADLTYQKILDDQTLCEYFVGIQMDEQAKKLGIILHQMMGWNCGVDYVLNYLRKSHQRMNLTNVHFTLFKQHLVEAMKELGLKESQIELITKRMDGYRSCIVNQDCLLDFYFQSPTLFKVQVKKYEVFLQRDPRFRNFPNVPTLLRHAHFLLKFITHQHQPLLTKIDLTTLHKNCVIQNEWLDGFRDNFFILIKNYNLDRLILQDYADLWFQLRFIVTNQQSIESIIGPQGLDQVQFKVQLNLQDNEIYSDHFRNAEYQLKSHVKKIVGFIFRNSSIYKANDLRVIHSPLKISEQTFNLFVQLIKQVMQEEKLCANLILFADQICQYYRSSICNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VZY4</accession>
    <name>A0A8S1VZY4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0780185</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VZY4"/>
    <dbReference type="OrthoDB" id="308962at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14312:SF1">
        <property type="entry name" value="BASIC-LEUCINE ZIPPER TRANSCRIPTION FACTOR A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14312">
        <property type="entry name" value="CREB/ATF BZIP TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="169"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="251"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="289"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8182401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="325" mass="38284" checksum="10F254E2249EC607" modified="2022-10-12" version="1">MQQQLQRHGSMEPLNDQNAQVGGNALDQNLLRSKNCILWLLCFCFLFCTIYLQALCMIPGLQVCSMNGIEAPVPFQQSIIAYYFFTLLFLYMIYYALENVKHSIVVYIRWPLLLYALAAIIIGVVSIISSYSLTTDDLQEGWNKMTQWRKQQYYESSSTNLLDENKRNLILISIYHFIYGFIFIVLFGLLFNYQTIMPQNWRPPFSSRLVLRQTLIYREGMIQQPKQDDEENQENKQDKPFQDYNHNEIRQQQQQQQQQQQQQQQQSIDQQPSTIFQKKNTGRRAFGKQVQNSQVQQQSQIQQSQIPPQPNPLQDQPEEGNQQPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W0W6</accession>
    <name>A0A8S1W0W6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutamine synthetase</fullName>
            <ecNumber evidence="3">6.3.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0820078</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-glutamate + NH4(+) + ATP = L-glutamine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16169"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glutamine synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDO010000082">
        <property type="protein sequence ID" value="CAD8183978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W0W6"/>
    <dbReference type="OrthoDB" id="1936100at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000011">
        <property type="entry name" value="Glutamine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027303">
        <property type="entry name" value="Gln_synth_gly_rich_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050292">
        <property type="entry name" value="Glutamine_Synthetase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20852">
        <property type="entry name" value="GLUTAMINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20852:SF57">
        <property type="entry name" value="GLUTAMINE SYNTHETASE 2 CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00181">
        <property type="entry name" value="GLNA_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51986">
        <property type="entry name" value="GS_BETA_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GS beta-grasp" evidence="10">
        <location>
            <begin position="18"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="GS catalytic" evidence="11">
        <location>
            <begin position="111"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8183978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="380" mass="43287" checksum="5AEBA5BC9E65B012" modified="2022-10-12" version="1">MTETVEHLRDFNYYKLNQNVTQVLAEYIWIDGTGERMRSKTKVYPTPITQISDLEWWTYDGSSTEQANTKWSEIYLKPVVYVKDPFRGDPHILVLCETYLPDKKTPARYNFRWIAHEIMEKAKDFKPWFGIEQEYFLLKRTGTTHIWPLGWPTGGFPYPQGRYYCSIGERNNFGRALAEAHLRACLNAGIKIAGVNAEVAPSQWEFQIGIAEGIEIGDHLWLARYILERLGEEFGIDINYDPKPILGDWNGSGAHTNYSDVKTRGEGGYKYIVNELIPLLSNTHKEVLKLYGANNEKRLTGHHETSSYDQFSWGDGSRGGSIRVPVITKELGAGYLEDRRPAANIDPYLVSAVIVDVALLNSTYVNQLKQLLVQSAKPLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W318</accession>
    <name>A0A8S1W318_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Enkurin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0810087</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000081">
        <property type="protein sequence ID" value="CAD8183611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W318"/>
    <dbReference type="OrthoDB" id="2123594at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027012">
        <property type="entry name" value="Enkurin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052102">
        <property type="entry name" value="Enkurin_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21490:SF0">
        <property type="entry name" value="ENKURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21490">
        <property type="entry name" value="ENKURIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13864">
        <property type="entry name" value="Enkurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51665">
        <property type="entry name" value="ENKURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enkurin" evidence="7">
        <location>
            <begin position="171"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="201"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8183611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="266" mass="31181" checksum="8963F150C0BDFF5F" modified="2022-10-12" version="1">MQESIYNLIPKEKTPPKKITKLYKSTIPGTIAPTGSTFGTQSLIIPVTNINGSYVIQQNRYRSESGILGKSKNQKLEPIKHKHMGNNSLDIWAEKGSIHRSIDLGYQHSDTKPQIPKLDDKPIMGLKQNKNFIATNAIDVILANPKRKQQEPDWTKKSTYGKVPTYLTQIKDTLKEQFYEEQYRKQLEQQEQDNKLQQMPEEELEQIRQGLKKKYDELNYQYQQQTHCRVFGTIGVKRRKENYEKELIQLEQDIEKLNKKYIFVQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W3F9</accession>
    <name>A0A8S1W3F9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0790161</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000079">
        <property type="protein sequence ID" value="CAD8182832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W3F9"/>
    <dbReference type="OrthoDB" id="285698at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8182832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="442" mass="51420" checksum="0A300CEFECDB24D6" modified="2022-10-12" version="1">MTTPYIYWLKESAQIQEYSVVGIIDGSGSMCEAWNDLCKAWNYFVSGFKSVYCIQYDDTAILQVSPQLTTQKGEGTNIELGFRELMKLIKSGNLLQNILIIFITDGIGEHDGIEQYFEEMNKEFEQLHSQGYQFKFHTLALGEEFSHPVAAELKMIIHNSGILSSQIDLIQNTKIDFTKVLQDIKDEVFYQMIRVDPQCQLTTFTGPVHRVTPNQVLFTNSNSIRVGGKQINLDKEGVTPEALDRFIRQIMTKLMEFNSIGKDVKQEAIQALQIIEKLKSRVTFKEDDDEIKKFIDQNIQKIEEFATGNLNLKEFQPLQAAKFMHDVQDLIIRDKKKMQRIKRKSVVHAQLTMHPQQPKQQVKDYYKELALKRLEQYEKQLNNQELYEDLKEFAKQNKVKTLDQIVYLTKTHDFQLKNIKEFIQTDEDTAIDMIDLFLISLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W570</accession>
    <name>A0A8S1W570_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0810001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000081">
        <property type="protein sequence ID" value="CAD8183445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W570"/>
    <dbReference type="OrthoDB" id="10043504at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8183445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="256" mass="30293" checksum="AE89219745E63757" modified="2022-10-12" version="1">MKLKKINTLMDFIKRKNMEFDNLINGINTYIDSLNQSFSLLKMVIRYKYSLQNERLLNLNSLQINYFNQQIHCYKLNNLYEKLQIDDNSIKLSKEFSDKCYYLKGKSYNHIQYIFEIKIQKSDITIRQINTIQSQQPLISMMQRSNFRQLIGGCLRFLNNYEDPIVWLDQALIIDPKYVNSLFDEVQVQENQIKNDDAITWLDKALSIDLKHVNSLNENGQCLRLLKKHNESQQLLDQVLSTNAKNTFSLNRKGDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W622</accession>
    <name>A0A8S1W622_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0760105</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endomembrane system</location>
            <topology evidence="6">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000076">
        <property type="protein sequence ID" value="CAD8181376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W622"/>
    <dbReference type="OrthoDB" id="294681at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027027">
        <property type="entry name" value="GOSR2/Membrin/Bos1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007705">
        <property type="entry name" value="Vesicle_trsprt_v-SNARE_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230:SF26">
        <property type="entry name" value="VESICLE TRANSPORT THROUGH INTERACTION WITH T-SNARES HOMOLOG 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230">
        <property type="entry name" value="VESICLE TRANSPORT V-SNARE PROTEIN VTI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05008">
        <property type="entry name" value="V-SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028865">
        <property type="entry name" value="Membrin-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="9">
        <location>
            <begin position="135"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="64"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8181376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="209" mass="24548" checksum="370F68465FCBF63F" modified="2022-10-12" version="1">MSEVFSSYEIEFEKYQQQICQLIRQTQINKSDETLNEISQVFSDLQNCLQQMDIESTSIAVSDRQELKSKVKRYKSEVEALKKQFRIIQDEVQSQKTKDSLFGEQDNQQIQFINTQDKLIKQTLQLEDAKKVCFEIESISNNIQVQLKNQSDILDRNINKMPEIQYDLGESNSRLSRIKSKMREHKIIFLIVFAIFLTGISIVLVYKYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W6B9</accession>
    <name>A0A8S1W6B9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0760155</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000076">
        <property type="protein sequence ID" value="CAD8181466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W6B9"/>
    <dbReference type="OrthoDB" id="288265at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="318"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8181466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="345" mass="40137" checksum="9993F95043B543B3" modified="2022-10-12" version="1">MATNEDRQYFKTGIVGFVFMYSMFKFGHYFNNNRKTKNQIKKMKQYPIKNVSEVAKEVQNQQKSNIYFIEGIPQKGSLYSTFKKIPVILRIKQRTSLFSCDSFLEPRIKFDWKEASSSSDYQIDKPLFFGLKDKQNQTEAYVSINDDTDITTALMSFGFKDEKRSLSFAEHIGNYATIFYSSLFRILGPAALIQVLFRDGSFRGVHIGYRDNEFGIKTDGFLSVLGQAVYNTETKKITIENPLALTLSKSNFIYQLEKKLQENGVAFKFWFTLSFLSGLYISRRVFLFHKKHPKILKKVIALFKKLFKRKQKREENNINNEIAKPLDNVTDEKKAEANETPTELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W7N0</accession>
    <name>A0A8S1W7N0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0860233</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000086">
        <property type="protein sequence ID" value="CAD8186248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W7N0"/>
    <dbReference type="OrthoDB" id="305883at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8186248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="16433" checksum="6C2F1A3ECA2CF309" modified="2022-10-12" version="1">MQDIDRQLDQLFELFKDKKTKLFSVDSFIAALQSADMTQNTKILQERIAQFKKQQLTKQEVLQLFQNDIKNRDICLAIFNSIQKKNQSYITVQDLQNLNQIHNLGYKDDQLNFIMKYLGKQNQITSEQFVQLLQQQHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WA38</accession>
    <name>A0A8S1WA38_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0880044</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8186894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WA38"/>
    <dbReference type="OrthoDB" id="306414at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15048">
        <property type="entry name" value="STARCH-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15048:SF0">
        <property type="entry name" value="STARCH-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="334"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="431"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="453"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8186894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="754" mass="88151" checksum="F34709513A247ED3" modified="2022-10-12" version="1">MRPNHPYTITFPIWQCDQWTAFRGRLKLSDKQVIIARWNNTIYLDEELAFFDSQHRIKPQKDIIMLKPQISLLPSILQIVRTNTYTDVLIENASQWFLTTGNLSKLLLIYFAYDLYTGCSPRDCTLENIIKNDDSIVILDFGLRKRTEKYCVYWNPLILKGKPCRSSYQWSLGIIYLVMTQGTQILNEIQKQITAWLKGGRLNIEFLITNKKQSVQNLISSLLNPENPIPWDQIPNHPAFREDIACQQILKEFQGNSIKTEVKSRSSSRTNSRDENSNQISIMPCPYGLKNPIISDQNIINQSLITKVSNLQKAHQDKVRNALGNMYSSILNRHTSTSQQNRQITQTNNSPNKYNNFFNSTKILPKSKTKPQNLGFKTFRNQNQVSTQNVDLDSKRQSSDITIENIVQPTQNQDSFNDNMRLRVSSVKVDTIRQQKPRKGSTHSVLLEEPKQQQKNHQNQVSKNQKEVQQIYQQQEILKVEEEKKIIKQQVLDQQQQHQQLLQIQQSKLQSQQQQQSQSQMLGNYDAENNIQLKKRNNTQFQQKYNRSLEAINVIGQTVAKCLTFFNALQNFWVIPLFLVFKRMLQLRKQIENLLSQKVNSFNINDWEEITSSTEFENYLNKVKQENQLVQSELSILLNSAKAKAEKLDKSRRDKVEWFINDDLNDKIKDVCNTYFHGQIYKNIKDKKGIAKSNIEWLKLQIQAQASIIISELPVLTCDKENFTYEDYLSALELTDEDQIQQYLNKNEHYLEHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WH98</accession>
    <name>A0A8S1WH98_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0900149</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8187997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WH98"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="48"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="50"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8187997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="160" mass="19345" checksum="D1CD758AFD120E10" modified="2022-10-12" version="1">MQREKRIPQKTWRYEEYSPPRRSSQSTNQNKRKRELSYYKIHKFSQNSQFTKEEDESILQYVKELGPKFVKIATYFPSKSYSMVKNRYYKHLRNKVFERKESQELNPNNQDTTQQLKNIILKPDNQKIEELNQLISSINLFPEITEITKSFIGELQKCLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WIQ1</accession>
    <name>A0A8S1WIQ1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0910186</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000091">
        <property type="protein sequence ID" value="CAD8188517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WIQ1"/>
    <dbReference type="OrthoDB" id="311131at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="264"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8188517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="390" mass="46519" checksum="DC9921175DAC7064" modified="2022-10-12" version="1">MSKICQKHKKFEEKFICIHKTRLQKEGNKFSCIKCLNWKKSDDYLKCIEDVEEEAKQNVAKYVEMQSKIINSNNEMKQKYLQSLTELCQKINQYFENQQKSITSLVEQQNQILTQNINQANSLLNERSVLDSQSLDNFIIFTTLNEANQQIIENKYVSINKCLEKQFNYEFAEIQCKVNQLINMEFKFGNIPKYEVEQFRIITADKKKLQFPYCQDHQMQKTCICTHEDCCKEQQQDYFCISCAMSNHDSHLKGNFIKTVKKIKTEQNEKLKIIKEQYEMLKQEVENRIDKELTANQQQQQENFVNSQTIRENLKRLEAQIKINPFDQNQNYIFNNINQNLLSYDQNMIKLEFQSKLDQLEQLKQHISTIKEEECQQQCQTTEEQSLTLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WLG9</accession>
    <name>A0A8S1WLG9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0940065</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WLG9"/>
    <dbReference type="OrthoDB" id="277011at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000093">
        <property type="entry name" value="NLI interacting factor-like phosphatase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="1">
        <location>
            <begin position="52"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8189515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="315" mass="36761" checksum="2F895A134DA2D820" modified="2022-10-12" version="1">MSNNQIIYQQQEEQRSLFSRLCACLQIFKDKKKRGSQNQYHHEIDTPKSSFILGQKKTIVLDLDETLVHSQFQPMDNYDFCLDIVVQSQNFKVFVIVRPGAKQFIDELSNFYDIILWTASLKEYAMPVMDYIDPDKKAIDRLFRESCTIIKGGLTKDLNKLGRDLKDIVIVDNSILSFALNSENGFQIKDFFYDTQDRELEQILPFLVWISQLPDVRPVSLQYQQFINSSPEQLNQRRNGSIVPLDQQKFYSVSRSFTMQREKIKQSSIIKTLTLSKNDEEEFDEIVFKKQINSGVIAKQDQTNDSEKETFEIGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WPT1</accession>
    <name>A0A8S1WPT1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0890198</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000089">
        <property type="protein sequence ID" value="CAD8187676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="299077at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1168"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="184"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="318"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="133"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1182"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1200"/>
            <end position="1209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8187676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1314" mass="150857" checksum="FCF0663D96C43747" modified="2022-10-12" version="1">MATDYRYSQIDNPTGAGREYGELDLVKIAKLDKLLSKIDNDFKKKQKEITKPKLVEIMKTTHEIYQIVLYEISEMLESAKLSELRQSLNKIYNGQNMILNYILKLNTNFEVQADKMLETLQKNIGQPRRKSVDQSAQQSQETNKLANQMVQLQPIVKKTVRKTYADKGTDTGDDIKDARNQANLLKKEMELKGMQEELDQDSQQITEFLKNRKIDLQAVGESKAETNLKEVLKDLGKKKHEVNDAEDEAFQNFEELQKQQQNQLKSLEGAFVEGIQGMQKQAQEKALADIKAGKAIMKNGVLVYMQEMSIQTDKTKIEQEYQDKLDKQRKENHDVQQKMIQLEKRIKDTLIERDAYAQQVGVLNGQLQKVTLEKKKLEEKQQEEKKAKDKQQQEQQAVLNKNDSEKIKRLVEDNIKMKLRIKALLDNIAALEKEMNRNFERFKIFITNNPNLSDEQKKAILGQAENLFKVNLNEKLNVQEMKELMAVDDLLRDPIFKQICGADIGAITRGVKKDEMTRKQKLAKVPFYDALFDPEFLNPDEKIIVRKVKKMVRRQKSDGEWVEEEMEVEEEVVVNAQGEELRKRDKPNQIQQSKQTQDFLEAHGGFAIGGSKINLPSKPVAKDGKEIKQGEWFEDKTGKKVRKLQGNEADEERFEVEEEYIDENGQKQKRIKQVRIKKDKNGVEYVEEEYVMPDGKKLKVAKRTIKDKDGVDVVEEVTVDEKGNKITKRQKAYKDADGNDIIEEEIIDEKGNKTKVKRKVYIDSDGNEVVVEERIDAKGNKVITTKRKNEYGQDIIEQQIIDKNGKIATKEQKIYQENGITVVEEVAFDAQGNKIIKKTRIKDGVEEVEIQNADGSVTIQRKVKNQDGTETIEEEIVDANGNITVVKKKVYRDAKGNIVTEEERIDAKTGQKVVIKTVKDSFGRETVQKQIQNKDGSIVNQEKNVQQDAEGNVIENEVVYENGQKLNVQRKIYREGGVEVKEEVIVDEQGNKKIVKTKVTKGKDGAEITETVTTNADGSKTVVKTTVDKDGNVITEIETVDAQGNKIIVKKKRNKNGEEFIEETKVGVDGKIEKSVKRVRADKNGNLIVEETTIASDGTRTTKITKLGTDQKATQTDGTYNDGKMSMDQVLQYLYELGLDKTKIEQIKNWVSLKQQIKTNKQQKLQQTQQQFNQQQEVQEEIPNQQQRPASAESQKSLDTLDDEEPEQDQADKLYNKMVNNKSGGMGELIRQVRNALGKQGNENISQEEFRDYMVKMKQVHAKCGDNCPHLKRFYEKLGFIQKKYKRRFLKMKDTKIEAKTKLPHLQNFSSIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WSJ8</accession>
    <name>A0A8S1WSJ8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1020193</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000102">
        <property type="protein sequence ID" value="CAD8192838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="311324at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="3">
        <location>
            <begin position="228"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="613"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="848"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1280"/>
            <end position="1423"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="161"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="357"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="756"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="899"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="935"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="956"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1003"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1283"/>
            <end position="1304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1353"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1393"/>
            <end position="1403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8192838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1423" mass="170154" checksum="9F1EFAA09F2923A8" modified="2022-10-12" version="1">MSYADFGQNIIDNEMKEVQQKAQDGWDIEEEDFPSDHGNQDQQQQLNNFRDYPNQNQKNNEWEIDDDIDDLSQTPAESQLKQEQQTKFQQDEQKSPEQIDMWGKFTNTVQQTQTIEKLIKESAPQKIKQTFQNLFGSQNSGSNNIIITSEQTQNSKVFASVQQVIDMKKLFELEKSELEDQMKLRRIQEQNELNEQKEELENKIKQFQIEKEQFNQQLKEAKQLFKLTELIEPQDNHEQIICKLIKENENLNNDITKLIETQEKQINDYEDNIEQMQQEIEYYKNQFIKYQTKYQNTKEKIRIEIPKIQNQLMTIKKLELHTQVTNKLTQFQKYYNTKQQDLLQYALQTTSKQSKQISSLKEDNIKLMQQIDELDELKDEHQSSLYIIKQLRVSEDKLIEENERLQNQNQDKENQNFELQNKLDNLKREYDQEKFNLNQLITQLQSQIDQINRNQVDKSSIKKLEDSLALIQEEKKTLLSSLENKETQIIVLEQQIQELNEEFTKQQNIDSNDIWKQEDRGWELDDSFQRKYDELELQMIQYRNENHELKYQLKEMEVRMLQQQHKQEAQVEQMHQLQMCLVDTEYQKNFQNAVQQERIQELEEQISIEKRKFSDEEEKVSEQNLQGDEDSQRAINQLRQIDYEENEAQREDGAWEFEDSLNVDIQEQENINEGAGQYQNIQLSQGQNIQQDHYEKQVDENSDFQDEIQQNDFYIQDDSKQNDQSDNNIHQQNDLSQEQQEQEDNQNIDQQIQQNIENDSEELSEKEGLLQNYDEENEDNYEKIEESQDYEINQNNQQNEEFYDEYQEQQQEQSVHSSQDNQVNVQEMNLNQNNLDNENIQDDYSEIKEGSQKQEEIQNEEGDSEYQNVEYESQENNDDQIQQDHQDQISSNDQIELQQEDKEGDQREYNSNEELNQEQEDQKDDVPYEIYQKNNENADQVQQQYQEQNDKVEQEQQQDDQQDDEQDEEQEDEQDDQQEDEQDDQQEDEQDDELGNEQKQEDEQQQEINSDEFNQEDQQNIYENQEINSQEKDMISQEYDQEQNNNSNKSLQQIDVEIDNQINQYQINDKGDIQKQEQIENELSNEEIYLNQQNIQNNLQIESNNQVINKTDELIIQDSNNQENQENNQNNEEIQIQNEINKEQEQLEIQYQLVVEPINNSNFENQVIEKSLGDLDVLDIHINNDQKKVVINSSNQIEKNYNIEIHSQKNALLSQDPSPSYQEIGVQEIISKPLLFSDPRSQEKSANDQEDEIQNDQEHYEIDVGLNEQKIENLENKENLIENPDEEEGNQWNVDDDIDFENELENPQVQIIEEKQSNQQIIEPKICDQSQNQQENERRNQEPLENWKVNQQFEEETPDVQFDDDQENQEDQSNNNNNPPIKNVEKLSQGQLDLDDELEEIDPWAEQQKQGENAESGWDLDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WT59</accession>
    <name>A0A8S1WT59_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MaoC-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1000103</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000100">
        <property type="protein sequence ID" value="CAD8191957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WT59"/>
    <dbReference type="OrthoDB" id="3592703at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019171">
        <property type="term" value="F:(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03449">
        <property type="entry name" value="R_hydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050965">
        <property type="entry name" value="UPF0336/Enoyl-CoA_hydratase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43437:SF3">
        <property type="entry name" value="HYDROXYACYL-THIOESTER DEHYDRATASE TYPE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43437">
        <property type="entry name" value="HYDROXYACYL-THIOESTER DEHYDRATASE TYPE 2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MaoC-like" evidence="1">
        <location>
            <begin position="20"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8191957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="141" mass="16270" checksum="3D5375F3C9E24B5E" modified="2022-10-12" version="1">MKLRRIQYFFSDFRIGQLFKLERIFTEEDVKIYANLIKDFNPIHIDEYAASQSIFKYRVVHGMLSSSLFSTLVGTNFPGAIYLDQSIFFKAPIFIDEQVIAQVLIQDIKLKKGRSILKLNTTISKMDQKIAVTGEAKILIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WUU0</accession>
    <name>A0A8S1WUU0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1020039</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000102">
        <property type="protein sequence ID" value="CAD8192557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WUU0"/>
    <dbReference type="OrthoDB" id="301579at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="96"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8192557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="242" mass="28994" checksum="953023BC2F521E36" modified="2022-10-12" version="1">MKFSRSTSTHLTNLTFRSSNPSTIQTPQSNSRLSEVNNKIDDLLKKSSTQINKQEFKFPSQEKKINQTYNHTLVVEQIQKEKKQLLTILLQKDSIIRDQQNKIAQLDLQNKNLMKQLQQFSNLQPQMEQLEEQCQQIIKQNEQLKYEKNQIQTSLNYLKCQNIQVISQELKNSLQQLHQQIKKYYICNYKEDYYNLEQSRMTLKMSDFDFYIILRECLDCILYMIKNTDHSLENYFNKNTKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WWD3</accession>
    <name>A0A8S1WWD3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T1060142</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WWD3"/>
    <dbReference type="OrthoDB" id="265776at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006615">
        <property type="entry name" value="Pept_C19_DUSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF24">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06337">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51283">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DUSP" evidence="13">
        <location>
            <begin position="27"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="12">
        <location>
            <begin position="373"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8194334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1042" mass="121918" checksum="B84378A914A9ABF5" modified="2022-10-12" version="1">MKYSGKDDNQEDSDDLQKTSISQYQANKLKQQAKEMHDMLLLLKENFEDEECDKYYILSKNWFEQWKQYVSYDQVVNDKPPDNKFGQIILQNYNNDLLETRTNECFKFYPLSSNPWNIWLKPNLQEDKDYIVISQQIQNYLNQHYRGTQILRNSIGQGKSKKVVVNLFKFNATLIIPNTIIQISQDNLIQFEKEYLQADESCVMKDLYAIIQKTVPTFRGNYNNQNGVRIWRYKNQNDPYKALFAEIKKQVQDLDFNDDQVFDFSGDPIENSQDITLQSLNLTDNDLVVIEFQQSFKPWCIRHPSVPVEGKCEGCGSISELPFPCICKKVAYCSERCKINDEKFHLPKCDPCGSDDEQVKQITINDKSVKGVAGLANLGNTCFMNSGTQCLSNTYQLTEYFITNKYFDEINEDNPLGTQGQLVRKYASLIKKLWCGDKNVVIPTSFKKAVGQFQPMFKGFQQHDSSELITFLLDGLHEDLNRVKKKPYVESKDNQGKPDFEVAKESWENHLARNQSIIVDLMHGQYKSTLKCPTCSQISITFDPFLTCGLSIPNKKQKSIQVKVIKSIVQIETKYLNFEGSKKTMSLLEFTKEQIIPEFKIDPNAELLFYTSFSNDIQGLIDPKSEINSVRKNSKRGFLVVKIINEEEKQIPTDDRIYLNYSQKALDGFGQQYKRTIFQQFYVIISKEFTLKQIHLAIFKALLPIFCDVISQNDISTPEQLRQYYEQNIQGKYYNINFRSQSAYWQQCSFCNQKNCQDCEADYKDETYQQIKTKMLENDQHNKIELIIFWIQSPFQHVKPADIYQYYQNQQYKKQMEEERNTTNNDENNDNQKTIQYGMKSQNYANNNQIQKVTLQECLIQSEQPEQLAEDNAWYCKVCKEHVQAYKSMQIYKASDILIFTLKRFKASSGFFKQKLETFVEFPVKGLDLTEFILNKNRPLDYEQELKQQQKIEEELPEHNENDKNDEKLLYDLFAVSNHFGGMGGGHYTAFGKNHLNGNWYSFDDAQVSEVDEDQIVTKSAYVLFYKRRSKQQNTQDFITQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WWI4</accession>
    <name>A0A8S1WWI4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1060167</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WWI4"/>
    <dbReference type="OrthoDB" id="1298661at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8194384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="252" mass="29468" checksum="5FCC8A4DC07BD865" modified="2022-10-12" version="1">MNSKSTNINKGNYLQNIRNMGQLNKVLKKGIVKVDENWKKIKSNQGVLLGGSNFLNKIYLDESKKLLTMAQNILFKNQEFLIFKHIYITQNKNLKGVIYKLVEIRIQLKIIILMLKSKGRAWKSNTLVLLHWLYQLLIEGNLSECAFNYVIKRMKAQLNENKQKILKENIQNINDDIEVKSVIRLDSIFKQIFLYSNLDLKYTGLQYNQLIQGIILSQQIQNELTYQWCNIDNNIMTCPSVYIGSIIMYINP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WWL0</accession>
    <name>A0A8S1WWL0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1030060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000103">
        <property type="protein sequence ID" value="CAD8193010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WWL0"/>
    <dbReference type="OrthoDB" id="293589at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8193010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="143" mass="16368" checksum="4610EA8BE5ADCF6E" modified="2022-10-12" version="1">MGSVGCCKNSPRVDTLKFDDVDSSSQESIIHKRDDQFSPRASSPGQEAVPLQINQRQPQMYQKHACISTALQSYSFKTISSEQHFSNFITFQALPSSRDQQSSKVQNIRSNFLEDLHEIQHQLSCEFQSNEQIKLPSQYRKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WXN6</accession>
    <name>A0A8S1WXN6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cation-transporting ATPase</fullName>
            <ecNumber evidence="12">7.2.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPENT_87.1.T1040042</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.-" evidence="12"/>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WXN6"/>
    <dbReference type="OrthoDB" id="425043at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140358">
        <property type="term" value="F:P-type transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000023">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000068">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006544">
        <property type="entry name" value="P-type_TPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047819">
        <property type="entry name" value="P5A-ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01657">
        <property type="entry name" value="P-ATPase-V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630:SF8">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12409">
        <property type="entry name" value="P5-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="167"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="190"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="355"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="395"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="898"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="930"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="961"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1017"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1048"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1092"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="domain" description="P5B-type ATPase N-terminal" evidence="14">
        <location>
            <begin position="28"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="13">
        <location>
            <begin position="226"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF12409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8193351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1130" mass="129246" checksum="4DE522AF4933740C" modified="2022-10-12" version="1">MKQPFINHNYRVQFKHPINIQQEGLISEITSCRISLFKTVVFAFLSIITCGLLYLVTRWDLRIFLFFRAKKCVPKSATHFLIIGQDQSQTLVKSNRSKDGMLFIEYRLYRYYYTEFGFDPIETKYQTMIQQEIRQQLINRNDQLDKFGFNNTEIPDKGIAKTLIDEVLSPFYIFQFCSVLLWFWASYQRYATVILITSLISIFITLYEQRRSFYRLQQLSKFNIPVQILDEGQMKEIQSINLVPGDKLIIKDGMIMPCDAILLNGQVIFNEAMLTGESIPVLKTELPNNKEIYDPQDAGKQFTLFAGTTCMETKGSEVIALVTQTAFNTQKGQLIRSIMFPVQNSFKFYADSMKFVGIMAILAVIGFIITVPNKIEYLLDDSISTWEFINEGLDLITITVPPALPTCLQIGISIALARLKSSKIFCISPQKVNISGKVTIMCFDKTGTLTEEGLDMYGIRMIENQRFSKIVTSIDANTDVNFIKGMATCHGLSQVKGKLVGDPLELKMFESTNCELIEEKDGRIRIRNNDRINVEIIKRFEFSSKLQRMSVIVKENGQYQAYMKGSPEKLRQLCNNQSIPFNFHQILDFYALNGFRVLGMAQKSVQQIDLDRNEVESNLNFIGFIIMENKLKPITTKIIKQLKDSHIRSIMVTGDNVLTAISVARQCGLIENQRVYLGELSEKKLNGKYFVSWKDFEYNQNELNEDTLEPQQQIINNLNEIQKDVDVQQDFEYLNQRSFSKNSVKNKSLLEDPPPVNIQLDNISEIYTDGDFMEEQPWNENENYVLAISGGAFMHLNKYGSQATLNNILEKTIVYARMRPEEKANLIQQLQKHKSLPLVGFCGDGANDCGALKTADAGISLSQAEASIAAPFTSQIQDISCVPILLAQGRAALTTSFCCFKFMALYSMIQFIQVTILYLKQSNLTDNQYLYNDLFTIFPLSMTMGLVQAAKINKFVPGSSLISFSVLGSVIGQTIIQLAFQLGVYLLLLKQSWFIPNEELNQEDLNDDSMKICFENTTLFIFGNFQYLMIALAYSNGKPFRKPFYTNFYFIGSTIFLFILAIVFLLMRIDYIDDIMGFIFQSYDDQYIMPESWTSLLGIIAIINAAITFIYEKLVVPKLIFKTKVQSIQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WXU8</accession>
    <name>A0A8S1WXU8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1070147</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000107">
        <property type="protein sequence ID" value="CAD8194758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WXU8"/>
    <dbReference type="OrthoDB" id="329563at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007091">
        <property type="term" value="P:metaphase/anaphase transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558">
        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558:SF13">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 27 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="119"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="429"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="463"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="497"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="531"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="599"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8194758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="656" mass="75949" checksum="9205ADFABE58A3BF" modified="2022-10-12" version="1">MIEEEGQQIKSQLEYQLIEAILDSLQNHMEQNAIFLAERLVYERDTEEHRSLLAECYLHENQPYKVCHILKECKSEYNRYQLAVAWFRSKKYKEAETALIGPQFSNQFALQTTNIPNGGFGHFLLGQIYEQMHRLDDAKISYYKALDQNPTLWMAFERLSKIGEPVTINKVFIEQKQKQYETSRQQSCNIYKIIGNLLKSKSNIGKSGSKEIEDVKEESLIIDNQLANKRLTQGVKPFSTTGVQTQIIHLDDSSNSQMKFSQPQQKKIGAKIIPTPGIGLQNYQSLLSQPFQLLQNQNKNYNTKKSDIQKVSSAALQSSPSLINLPQLLKLFAHPYQLWTNYSVDSIANFQKLPPQHYRSGWVLEKVARSFMDQVKYTDAERVWKEMRQIEPTRLEGMDYYSSCLWHLKKQSELTYLAHSCLQISMQAPETWIAIGNCFSLIKEIDNSIKFFGRAIQLRKDYSYAYTLSGHEFSQNENFQQAKKSYEAATSLDQRQYNAWWGQGNMYYKTDKYDDAIKCFIQAIRINPNNPVLPTFLAMSYAAKGEHNEALKYFEQSEKLDPMNGLNKYQKANSLIKLDLYQQALSELQCLSQIIPKEAAIYILMGRILKKLNRIQEAQNCFNMAMSLDMKDQAKIKGLMESLSNPNSEFNDDFDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WYD1</accession>
    <name>A0A8S1WYD1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0970166</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000097">
        <property type="protein sequence ID" value="CAD8190886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WYD1"/>
    <dbReference type="OrthoDB" id="532484at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029147">
        <property type="entry name" value="CFAP77"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28617">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28617:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14825">
        <property type="entry name" value="CFAP77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8190886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="215" mass="24637" checksum="62A6823BDE9E83D8" modified="2022-10-12" version="1">MQTAPPFSHNHSNPLLMKDDVGKAKPSTYNLPNQDFIYGQPLSRDKEGAKEVTMTWKFHQESQDRVPNRDFAELNKQSINNGLVKAHDMYKYRQTHDARLKINKGTNIQAIELPEEEFRYGRKNRPSTPMKLVMGNSYGIEAESNILDKYQQRAGSQDSKLTTSIVKSNKASQLFHESNHKKLAAIQGVEKKEPFKMEKFKSVNPKINTNLSTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WZ02</accession>
    <name>A0A8S1WZ02_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1040061</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WZ02"/>
    <dbReference type="OrthoDB" id="294692at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF30">
        <property type="entry name" value="MATERNAL EMBRYONIC LEUCINE ZIPPER KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="21"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="375"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="377"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="397"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="415"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8193389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="426" mass="49681" checksum="B2990440B32F6D8C" modified="2022-10-12" version="1">MMSLDIQNDYLEFASILDDKYYIIDTIGFGRYAKVKLAINMEDKQKYAIKIMKTDPTTSQCRSDTFINEISTQAEFDHKHIIRIIYCRMNGNYKKIDGRIQQVSYFVMELANQGELFQLLEQTQQFSEKFARNIFLQLIKGIEHLHERGVVHRDVKAENILFSNGVLKLADFGFSTKTIDEKGSRIQFEISQHIGSPEYNPPELYNIGKQKFYNPEQADIFAAGVILFTMVIRSAPFKTSKSTDPYYSLLKNNKQSFWKIFSEIADSSAQFKDLIEKMLDENPLKRITIEQIKQHPWIQGQMFGQTEFQKELKNRYDMILSQQIVKITNKRDTKFSTRKSIKKVETDNISKNVQKQCYENIELIDKINAKLQIKQHSPKEQFKQQSNKSEKLKVGIQQKQQNQYSSRTNQESKRSSPGSPSNDSDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WZB0</accession>
    <name>A0A8S1WZB0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0980180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000098">
        <property type="protein sequence ID" value="CAD8191296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WZB0"/>
    <dbReference type="OrthoDB" id="308278at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8191296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="209" mass="24807" checksum="515682C0DC988BF8" modified="2022-10-12" version="1">MQNYSSDEEISSIPEDEYLDYDKQDFEAIQFANNKQNIYEDNQENDNENDEDEELEKDEKLQKTGIKFKLPQRNQIENKKALSNLTNHKVLIETTNQLKNISKLQNRPSTSTTSSYKPIYTSSIHQSEQMQKKYNYKQKSFNTLPKRSDPVARYQQMSSQWKKTKFLQNTNGLKEGRKLNLDTRIEQQKQELKESGMFHRYIAKSQLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X0Q1</accession>
    <name>A0A8S1X0Q1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1070014</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000107">
        <property type="protein sequence ID" value="CAD8194497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X0Q1"/>
    <dbReference type="OrthoDB" id="9450131at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="331"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="365"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="81"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="371"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8194497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="413" mass="48705" checksum="FBDB4162E2BFA543" modified="2022-10-12" version="1">MKQQNLFTCQDIAHEGEVIQGFCLNLECQDSKSQFCLQCGLDPKKHTNCKKDLKGFGLIQSFITKFNQYILDLTIQLNQSYSSIKTKYEEFIKQLDNMKKQLLKISDDLNQQDYKQIKDNLQMIKEWYQYSNNQEEIMKQNQIGTQLLSIKKMIQTLDFDNKQQQQQQIKSIYQDNQITLEQGIELLNQQKWQEANQKINEYLKLLEKQSSFATFFQGISLIEMNQNGKGIIMREQAKKINNNLYKDILDYSDLELKKNPKNKFILIAKSYALDDEKKYQLAIEQCERVLIEEPKHLHALYRKSFTLYNLKENSQAMQCIDIALNYDSKYIVGYNIKGNSLNNLGKYNDAIVCFDKAIQLDPNFALAYYNKGALLSQLKKYQQAIENYQQALVYCKEDQNEFNKLIMELRFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X0V9</accession>
    <name>A0A8S1X0V9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1000054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000100">
        <property type="protein sequence ID" value="CAD8191876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8191876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="71" mass="8215" checksum="EAA9CF5ABFA75F64" modified="2022-10-12" version="1">MDQNEEQQSPMQSPQLDPIDLPDQSSPRQPESPKMDRGSPDHASQVSEDIEEIIPEEXLDFINLKYQYFTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X5Y0</accession>
    <name>A0A8S1X5Y0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1130035</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000113">
        <property type="protein sequence ID" value="CAD8196571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X5Y0"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF69">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_38, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="4">
        <location>
            <begin position="73"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="73"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="3">
        <location>
            <begin position="76"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="125"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="4">
        <location>
            <begin position="128"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="176"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="4">
        <location>
            <begin position="183"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="248"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8196571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="364" mass="43500" checksum="5E43FE5673CD756A" modified="2022-10-12" version="1">MEVSEDVQSLFSQESIDEHEIIRPNQSLQRKGRKIYLKNIQKQESQSNKLVLDVTDTISIKQKSIRKRNKIYENKKIRKPWSTQEDHQLEKSLKLYGPNWVQIAASMCNRNPSQCAQRWKRIKPQDLKKRKPFTKEEDQLILQLVSKYRRNWGKIAKFLPEKTSKQIRERFINKLNPQIKFEPFTEEEDHIILKAYQEIGSKWTKIQDLLIGRPENMIKNRFYSYLRQKYLKIKNPYYSIPQKIPINNKDQQSKIDQVQKQIEEKQFRDNQQNKENYNQINNDIQEMQSQLPIQINPYIQIPCPMLLGNFSYPQYQLYQTPVAVIYSPIQQGQLIQQNTSNTLFQQQIFMGQQVTNQLLFLSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XGC2</accession>
    <name>A0A8S1XGC2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1240056</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000124">
        <property type="protein sequence ID" value="CAD8200310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XGC2"/>
    <dbReference type="OrthoDB" id="310342at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8200310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="166" mass="19428" checksum="6B3A326A8CC5A68A" modified="2022-10-12" version="1">MSQIQSINLDQGIKESQKGILNDKFKRKIQFLQLVLCQNQTIKTAATLSKINFATAKVVLRNFRNCGFIKNSDKDYEKQIDMLRQIACIKSEIKQEKIQKREQEFKILCQKIKTIQPSTVNNVIQSQMKIKSQIRVFQEELQNQEQIQLQLVKSILLEQIKLMKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XH65</accession>
    <name>A0A8S1XH65_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1230058</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000123">
        <property type="protein sequence ID" value="CAD8199992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XH65"/>
    <dbReference type="OrthoDB" id="300213at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="47"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="170"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="379"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8199992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="432" mass="51894" checksum="3BD07A21B5B25897" modified="2022-10-12" version="1">MNEREFRMSLGAFSSNPTSAPQNAYATALKALQEKLKGVEGLKKSIENPSDNQIDQLKKQINDLQNENERLQYLLMKTNQESQMNFISSQQCEVPQIERLQHFKDYQDRLSDMIRKYEELKHEKQEIQIANENLQRQLSVYKSNEKGQIHKNKHKKIENNTNIQDFKTKLDIKDQQLKIQEKKLEFLEIEKEQILQEYTNYKTKYPPMKQHDFEKQIHQLKILLEEKDKQYTKNIDDIERNKNVREEQMSRRIQDLIGKCNEYQQIIEQLTQDYKDLSLKYQQVKIKLDYQERQHRYINRKYSQGELKQDYKEHKNVNRIDISPPLTSSRLEQSQLKQAIRECLEDMMSTQNYNCCSPDRRSENRKQQQILSPMSTSRQYEIEIQLKELNEKYENLMNQAQKENDLNNKAVIRKQLIEIAGQIKETTKFEKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XI79</accession>
    <name>A0A8S1XI79_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1250019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000125">
        <property type="protein sequence ID" value="CAD8200564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="282545at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035738548" evidence="1">
        <location>
            <begin position="17"/>
            <end position="1304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8200564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1304" mass="148539" checksum="4EC1A1B499455D88" modified="2022-10-12" version="1">MKTILLVLLSVQILLAQDPWVTHYTAFTSAEINDLDGWVVKKAFKGNTFSKCDKISLVGGYGAFGKGATALKQLTLPPHYKLKINIQLWKIDSWDNEIMFVLVDGFIWQAKWHYSEGANLCGAANDWKEAFYNIEFEVPHNSPTVSIVLTSNLDEDALNESWAFRDFKLSFQRCHPECAVCGDNKPDNCFFWTNVATNWNKQISLEGWTLDGEGKAESNECAGVQLFGGFGKLGRKANLWKRFTNLPPHFQVKVKVQMWKIDSWDNELFLMEIDDQEKFRQAFAYNEGVDLCGVDTGAKQGEGWAEKIVNIEINVPHKFPEVKVLMKSTLDEPPENESWGVRDFQLFAAQCFKGCTGCTGXTQDPWVTHYTAFTSAEINDLDGWVVKKAFKGNTFSKCDKISLVGGYGAFGKGATALKQLTLPPHYKLKINIQLWKIDSWDNEIMFVLVDGFIWQAKWHYSEGANLCGAANDWKEAFYNIEFEVPHNSPTVSIVLTSNLDEDALNESWAFRDFKLSFQRCHPECAVCGDNKPDNCFFWTNVATNWNKQISLEGWTLDGEGKAESNECAGVQLFGGFGKLGRKANLWKRFTNLPPHFQVKVKVQMWKIDSWDNELFLMEIDDQEKFRQAFAYNEGVDLCGVDTGAKQGEGWAEKIVNIEINVPHKFPEVKVLMKSTLDEPPENESWGVRDFQLFAAQCFKGCTGCTGPAKSDCTSCGQGFDLVNGECKEGIKWMTLNRFFFNDEQDFQGLQDWXFHLIINQRSIYNCGTKWHYSEGANLCGAANDWKEAFYNIEFEVPHNSPTVSIVLTSNLDEDALNESWAFRDFKLSFQRCHPECAVCGDNKPDNCFFWTNVATNWNKQISLEGWTLDGEGKAESNECAGVQLFGGFGKLGRKANLWKRFTNLPPHFQVKVKVQMWKIDSWDNELFLMEIDDQEKFRQAFAYNEGVDLCGVDTGAKQGEGWAEKIVNIEINVPHKFPEVKVLMKSTLDEPPENESWGVRDFQLFAAQCFKGCTGCTGPAKSDCTSCGQGFDLVNGECKEGIKWMTLNRFFFNDEQDFQGLQDWIPSNVFQNQNPFSTCGQKKLFGGYQRFGVRAKAERNFNLPKHSRLRIQFQFWKIDSWDDEKFQVFVDGKVVFERSFGFSTPGQAKICGAPQSTWMTYFFNVDVIIEHSNPTANIVLSSTLDQGAEDESWGFREFQLLYELKEDCVELYTECGFKGTKYEICRDTPSLAREKISQVKSIKIPPGVVVQGFDEEVYKGKTVKFSQSQDCLEEIQFSFIQKKFEIIQADDSVLAANLRRIRFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XJ61</accession>
    <name>A0A8S1XJ61_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein tyrosine phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1270003</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000127">
        <property type="protein sequence ID" value="CAD8201216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XJ61"/>
    <dbReference type="OrthoDB" id="282898at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00047">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF449">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="1">
        <location>
            <begin position="21"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="2">
        <location>
            <begin position="189"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8201216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="283" mass="33804" checksum="DA24659715C7D809" modified="2022-10-12" version="1">MKYEMAINLQEFVKSFNQRRLEKEFSYLCQITETQEHEKLFFWNEKIHHLNRYIDILPCIHTIVKSDVNEQFYINANYIRGIDNIDKKYIATQGPIHSTVNDFWHMIWTNEVGLIIMLCKLYDHNYKQCEKYWPDISNNYGPYEVKLKKSEELKCGLISNQFIIKFQQQEKLIHHYQWNGWPDLGVVNEDSFVLIDFLANQANSSILTNKMTVVHCSAGIGRTGTFLSICQIKQLLDQKEAKLSIFSIVRRLREQRAYMVQTAEQYQMVYRFSLWLIQNLKYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XKX8</accession>
    <name>A0A8S1XKX8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1270119</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000127">
        <property type="protein sequence ID" value="CAD8201388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XKX8"/>
    <dbReference type="OrthoDB" id="307942at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001434">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="225"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8201388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="487" mass="56720" checksum="68CC751E9D8FBA8C" modified="2022-10-12" version="1">MQITCTQADHQNLQIIGFCIDNTCPNQRPYCHFCLPSHVQHINKLTSQELLSGWIKERILMVQNIQKNVQECKIALDSLINVFLPYNNFNIQQLTELGLQQIDQFIKGLCQMENCEEQLFKQLKQAIDQTKSNTNEIIKKTKNQTNIKQNDNLQNRQQQPKHICIVEQNQNPFTFDLIKQYSIKQDEWCIAIAFNKDQSIVLAGCNNDIKVFKHVQGKLNQVQLLSEHTDIVNTLNFMKNTNDFVSGSDDSSIIIWQAIGNYQWKCQQKLNGHSDIIYSLLTNNTDDLIISGSDTIKFWVKQDQWLCQQTISDHTYFVCSLSLNEQQNKLISCSYDSQILVIEQQKLDKKWIVTQKITADQEGQRLCFINDFQFTFQPYCKDQIYVYDMDSNTKKYSMTKQIAVKCSSSGENCFFPQQYINQKCLLVNKNGNYVNLIRKKGNGDFTVQQSIEFGQFFIYGQLSDDGEYLITWDSGSKEIQIRKYRQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XPR8</accession>
    <name>A0A8S1XPR8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1330031</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000133">
        <property type="protein sequence ID" value="CAD8203105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XPR8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8203105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="422" mass="49464" checksum="0B604E0EA193DF74" modified="2022-10-12" version="1">MQNHKCRFCTHKCQCLYASIDQISKKGNLIQTMNISLYYKHGQSQNYFFSRHINQIIRKEKHTIYTRVEDYKILDNQEEYLRQFYHFPNCQHQLKQLIQYYKFSIDHPMLYFLELKAIIVKYISSRSQVEYQWAINQIEIEANKEGGVSPKKSKKKSTPLVKQELSCFLKELNDTQKSTTMLQLLKNVEADRTLEKDLQKQFTQSLVEQDLTKAMQFSTFLQQKELQKQKESIIKETNTNSRTVYVGNIKKQLNLQIGTPRKASNVITNNFSTGCSTHAHSNMNTNQNNGGISNNSNHRIVVPNFHFFNHNINPSTQTSNNQNTVNHNFITKTSIVNSSNGNVLKPQTIYKMQPLRLMEVPKLKIKLNHQDPKSERKYDRIRENRIDCLTQRIKTQLDESNTARGSVNPKAIVIKRRQNFQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XSC3</accession>
    <name>A0A8S1XSC3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1350134</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000135">
        <property type="protein sequence ID" value="CAD8203990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XSC3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8203990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="91" mass="10548" checksum="1A569D5AA0E4DBB2" modified="2022-10-12" version="1">MRNEKNKINQPLFLMMSQLGESEKLSCLTSGFVDSALQQPEGNVDHDIQGNQKERKKERKKETKIQGLVTRTTLRYLVKASYLREECVCMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XSP1</accession>
    <name>A0A8S1XSP1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1360129</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000136">
        <property type="protein sequence ID" value="CAD8204281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XSP1"/>
    <dbReference type="OrthoDB" id="301195at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="286"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8204281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="562" mass="65962" checksum="C779EAD78EB0E42D" modified="2022-10-12" version="1">MTEESNSIFSTIKYYKNKLKFPTFPKQSNEIQESKEQRDLLFCNSKQRKEESYRYLKEWADNRGEICEKNKAQIEQLSNFCEYQIKQSSEYLSIVYKFFNEKYFHEQQYGQFLLTKPQPALKQNQLSQQLYSEISQAISIFEAVNLKRCEKVSNFSHAISTLILKAEILSDLLDLEKRSNQLLSCINVQKKQLQKQHSLSIQKFKAITDLFPQKQSQRQRKPIFDNPIQNEQQNQNIELKQNQEITHQCEEPSTIKINDISEVLADDQIQINHLSEQSKENIFDKSQLSVQQQGSSSDAPQDQEQNNKKVQQKQFEQSQQRSQSQTLPFSKALVDNQVVKQQSTIDTYKLMMSYMATQSYTIKLINQLIEQVKQYWKHVLMMEQKRTKWAQDSCQIYKDGILDVYNVDVELPQFKLELKEYFNLINIYPGSNSFQNEKDLDEFCKSLMIKELGCQAARLLLIKEFKVNVIDKNSELPSILVLTIDKFVGCWLKYDDSIDAIDTPIFHYPIIEISIQKIGELILDIIPSKNILFMNINNGKQKSKIKFACIDDMEEFITIIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y2H7</accession>
    <name>A0A8S1Y2H7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1480148</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000148">
        <property type="protein sequence ID" value="CAD8207843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y2H7"/>
    <dbReference type="OrthoDB" id="303988at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32114">
        <property type="entry name" value="ABC TRANSPORTER ABCH.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32114:SF2">
        <property type="entry name" value="ABC TRANSPORTER ABCH.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="834"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="241"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="396"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="491"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="834"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="850"/>
            <end position="863"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8207843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="905" mass="109220" checksum="E73A72562AA0E612" modified="2022-10-12" version="1">MDRSNSIPRSNSSRYIPQSLPSSYRQITAPTKFSNSQMKFMNDLVQQQPPSQRQALNSQLEELTSQEKREIQQKLENGEVLHGLSQLPEGANMTHKFIVKVRNNDLQNQGFQIIKQSQSPQPRNSLQQCPECYNHLKKLKEQDNRIESLLVEMNRMSQFLIDKGKEIDVWKKQYQIIQKELLKPKAYDNLDKKEIEDLKRKLNQKEYENSLQQTTIKQLQVNQDQNNDNLELRAGNNDLDVDQLQKQLLDIQQDNEILKQDNDRLNSKVERLKNELSNTKNEVTTIYKADPETERLFKELQSKYSILQSQYFTLQEMNQNVQKEIQHETKIIYQSNPDIERQLQELQTRYTLLQQQFYSLQETSKIMQKEMQYETKVLYQNDPETDQLLQKKIKQYDELNTRYLLLSDQLQVYQDKLKQQSMHETRTIYQPDPETERQLQLKIEQYNQLQLRFNQLQSQYYIIEGKSKFVQKELIVDPEVERLLKMKSQQLETMTNKCILLQEQIEKQQIQIESYQTKMYQFENRKSETENYLLQIQSLKDQLRSQNADIEQFTRKIYQYEQNNEQDQILKRQFIDINALYEQELSLNQQLKRKIEEFKNVQQQQNTIQYRIKELEQLLEQKEDRIKQLSLELDQYRSDIAIYQQRISTVSIDLENTNSLKEQIQVWRDKYEKLEQKQKLLIDQEVNQWKIRYEQIEQRYVSLQSNYETIQITLNEYKENEQSEVFKMEIKRLQKTVIELRSEIDKLLVQASDNQWLQKRYEEQQLLIETYKSQIEQKQQIQKEYIYQIDRSESNSQKFIKQSSVQQINIESQSRYPYSNQQQQMKVYEINTHQPRTSENANYSSRPVESNSSKQKTSYQQTQFFETSTNNVNDINKKKIEQEFNSQRWNNNYEMVIDELTKTDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y347</accession>
    <name>A0A8S1Y347_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1490103</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000149">
        <property type="protein sequence ID" value="CAD8208035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y347"/>
    <dbReference type="OrthoDB" id="287418at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="106"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8208035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="190" mass="22598" checksum="21EB6856DE863BDC" modified="2022-10-12" version="1">MKAYRSPCQSFHTVRTSQCNFATPERLVKVQTNSGVKCSPFQKNENQFKNYQSNTVAKKKDSTNLYDYISELEQKINKLEQSQCRNVNNNYPEILQLEKRMLILLNENCELKSEALMNQKLVYENEQLKQLVQEQQMKILEFGEQYNLLFDSHQKVCEEYRILESKIQTYELTMSIRKHSSFSEYFKTSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y5I8</accession>
    <name>A0A8S1Y5I8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1460009</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000146">
        <property type="protein sequence ID" value="CAD8207024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y5I8"/>
    <dbReference type="OrthoDB" id="292132at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051242">
        <property type="entry name" value="WD-EF-hand_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44324">
        <property type="entry name" value="WD40 REPEAT DOMAIN 95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44324:SF4">
        <property type="entry name" value="WD40 REPEAT DOMAIN 95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="71"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="2">
        <location>
            <begin position="171"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="2">
        <location>
            <begin position="271"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="2">
        <location>
            <begin position="313"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="2">
        <location>
            <begin position="358"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8207024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="975" mass="113206" checksum="7FC711A9D1B1FBCD" modified="2022-10-12" version="1">MKNQDYEDYDQDYEKKEIDIMEKLHKKKMAVIERCFPDKNSKGQGLTIQQFLKVMLEHLDYNKDSKEETKKITLALIELFKEIDVNGDGTMEWVEFSNHIIELGLLRNDRSFKDVIKSYHPAENIKDEQKHETVIDRVYFFDRLKMLLVLEKESPKFKVYNSNTSELIWNVNAHKGYVLSAEFIPDQNLIASSSNDLTINFWDSSSFNLKQILSVPEIQLCMRYARWSSSQSNFLYTGGSDSIIHIYDTYDLKERGTLSGWNPFIKRDSQQYGHSSPIGDILAIDQQNTLVTGGLDGNICLWDSATHQPKKELRGHEKGVYSLDWSDWSPMNQCLISAGLDHEAFVWNTYVKEKIFLLRGHNHPLVGVKCLPRTSQVVTADISGMVKVWDVRNFLQIQTFNVPADEIQAFTLTYPKKQIVVGARKMFFYEYDEPKDQMLTDEKMCLKVLYNQTLTCFITLHPDSVKTWDARTGKLQQVYRELSVAELTTMIIDARQRKLFIGDSEGRIFTVNIKNGAKMKKFERHHKMITDLAHWSNDTNRRVISCSREDTVNIHDEDSQDAHKSCRYKMKQHHLSVNSLHVKQDSENLVSCADDGAIFIIDLVSYRQELVWKCQYELKKVMFLESHNCIVSVDSIGNVYFIGVLESKFKSKLLLQKTYKAISLTNQEETFPVTSINYHDNLLYLGDELGNLKIWNIKQVLDKVDLHQVEQKIKTRKNNDADTFVTAMDYGQEDIAQIFVIGDIMEVGYQRKAHQDGITYIEVCKDGSHFATSSFDCCCYLWAFKNGQVSKIGALILGHDANWGYRIDEISRAEAAEQEANELLEELKDTIIPTKVEKNDGQISSSKQQLLDRLYGSKQQKDQQLQQNQAEKEDKKEMALNRAKNALKIYEQFKQKQFTYQNQQNQNDQDIKKYDDSEYKDVFQQQDYDEEEEDNYNILTDLQEFKQQQLSSGVYSSKNAFKTKLIKGHHKSKFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y6Q8</accession>
    <name>A0A8S1Y6Q8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1520127</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000152">
        <property type="protein sequence ID" value="CAD8208973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y6Q8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8208973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="63" mass="7564" checksum="3597CF53BDC0DC0F" modified="2022-10-12" version="1">MKYSLFDAKQMKKLGLLCDIEKSKRKKQQKNFSIQFRRKSLKGYCFKYQIVKLWVGNVLKSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y9F2</accession>
    <name>A0A8S1Y9F2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1620048</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000162">
        <property type="protein sequence ID" value="CAD8211116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y9F2"/>
    <dbReference type="OrthoDB" id="306069at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="184"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="291"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="358"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="376"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8211116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="401" mass="46031" checksum="81E14BEBCB21A371" modified="2022-10-12" version="1">MDTPNIQAAGARANKQTFDQAPSTRQVMQNKSDIFGINAPVPAPQASRQSARPKTDIFSNECQAPKQQEVRQNKTFQSQIFSQEVVQNAPPPPARSNQTFENSQQEVKRYQKKNNDNRQVDHGKDFLFGKSDFDEYKSLKRAATLSEFKPKLNYDPQNRRNKELYGNEIAREHLPTKEVNLEISSPQKTKKQSQQQQEQNPSNRKLMENQSNVFGDSQTKVVQNQKVEKLTASTQKWSTVGGQSNNQVKEFDPDTYAKNRKEAELQSSVFGEQPVKVQPVAILEQQEESETQQAQQQQQQKVQKPKERLIPNNQTWQQTDSVKNMRDFDPTQSQVKEDVDASQKKMDALKSALDTHQFEAQQIPANQKISNKNLKQKKDIIQREDKQTKDKTTTKTQAKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YA20</accession>
    <name>A0A8S1YA20_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1510133</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000151">
        <property type="protein sequence ID" value="CAD8208682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YA20"/>
    <dbReference type="OrthoDB" id="1668230at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004694">
        <property type="term" value="F:eukaryotic translation initiation factor 2alpha kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001126">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000818">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF136">
        <property type="entry name" value="EIF-2-ALPHA KINASE GCN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0652">Protein synthesis inhibitor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="245"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="213"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8208682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="583" mass="68445" checksum="9D39D3DE1850EA1C" modified="2022-10-12" version="1">MNQGEHLNKQEDDQKFTDGDQTFINEQTDDDKRFSNTVVDNKEQSGLEALLELDNRNKQEFLSSEEKSLFQEFIVICQIRKDKYMSEQDPHLQFLMDKAVSKGVQKILKAQKIQDKEQWQKEQQRLMRLFYSDMNIGFITSTGQLKSTVFIYLHQSIASYLDNRQINLNFNKEDLKIIANYFSIDERNKSSKSSPEFTSPILTKEKSEKEQFKQNSAGSKTISQNEGEVENQLKRSLPLCHQSNWLKQEFIKKDYFKKQFLILQKTNENELKSGVQIFFVYRIKDDQIYAVKKSQLASNVISFFDVFSTVNLSEITNDQRKIREAKILSKLNHPNVLTLYDWWIEEEESGYSLYLMSEYCSYPGLKHQTNDLLNYAYYYLNPMNCKEKIEQIKTIMLQIMDGLEYIHKRGIVHRDLKPENIFVTYGIDCQLQVMLADFDQGKDVRESKLSIITDEKLNDQDLNSIKSRNTINSGTSGYATANSQINKEYDKMDEFYALGVTLLHLILAFPGEPKNRNLYTNTFAISDISDVLLLFDSWAVKLVQNRHPEFNFQQYFHLMELAKALLDKKVKEHDHIRKIIQSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YA30</accession>
    <name>A0A8S1YA30_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1610066</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000161">
        <property type="protein sequence ID" value="CAD8210925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YA30"/>
    <dbReference type="OrthoDB" id="309800at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8210925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="641" mass="76881" checksum="AAE2AB35AE1783CA" modified="2022-10-12" version="1">MYEIQDLDQKQGNTYQNDQQFKNLQQQINYNIQPKFQQNSELIQYEQPLILQLVLNKIESLGKTISQVLAIEMFKQFTTKTRGWSSYLLQNEISEQRRNFLNLQEDCLKNGLQEEWQVAREEFKKFLKILEDLLQNQEHKQLLQNILQPPPALSFIMIQPDMKFFNALENYHYQSKINFHNSLQLDNTGQDLLEKIQTYKNELSKFLYFKFIEQGIRHLDQLLNFVLIHKIQNNQFTLQLLNDYIRQLRTENDKQQLLNEIQLIIIVFPELAQYFQEIYNIINDELLYFDGRIELKELQFEIVMLIYDYIITPTRVLNRKLSNKTICVMQQLNWIYQTGSIQTKQKQLKDLKKYIKQNHSQQLQLDLDYLSDLFISTKLKLLMKMIQQINVLQDSDIKFEYFNFHAFSFQDQDIIQQCLKQEDGHFLNIYHEPSIDEQQINIQQVFFQFNHVIEFAAHCEMDLNKNAQNNSLEELKNKIKDSIQSLANQITSTKTITKLQALLNQIFWFQKLNGNTFPVLYQYNQEINDIQLKKQDIIGYEEVVPNLYLLLLNFDQEKDKDILFQKKVKEITELISEDYAVDFSDLIKEIEQFDQKNKTAFIKNAKQYYYKDFKVHLLNLVQYGTKQFQEAINYLIQQVDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YA44</accession>
    <name>A0A8S1YA44_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1590103</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000159">
        <property type="protein sequence ID" value="CAD8210510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YA44"/>
    <dbReference type="OrthoDB" id="309323at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="52"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="214"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="316"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="503"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="572"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="14"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8210510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="619" mass="73877" checksum="9F3D2EA607FB57E4" modified="2022-10-12" version="1">MNTALRQPLSSHNSIDKRENKPDYKSDLKSFSFIKSNTLNVQSQQQSQQQILLQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQLLQQQINKTPSQTTLTTNTNNKINNQIQDLETFIKEIDRLQLENQQLKVKAQEPQLIIQSDPKVAIENQDLKNQVDYHKQKYELQEKTHNETVRSMDLNWKQDVVTMKFQMEQQESKHRQALKQEQVQAKQEIARLQQNLTQISNSENKNQNLVQVLERQLINLKKSNNILEEKLSQAIQQFDSQSKDQEFTLLKQQAIITSQEQHVSDLKSKLGCQRDQNQGLNNELERVKEITEQKKQNENMLTNKIKQVESDFDHLQQTYQQHKEQLQLKINELTGMYGKSQEQICLLQQQQIREQNLIKRLEVNLENEEKNHEQEVKDIHKSKQLLIEFLEQELVGYKEQNQQLKLVNQKLENQHQEQQQYFGKNFRELECKTNMLGEECSRLNNIIKQKDDQIYRLQVQLKDLMNLNGKNQNQEELLESLQIKEGEINILKQQNESLINDLSRQTDNYRSLAGQHKQLQKFAEDAKMNNITMNEFMNKCELQEREVERLRMKVLEKSQLLEKVQKENLEYQAKLKSKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YB97</accession>
    <name>A0A8S1YB97_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1590086</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000159">
        <property type="protein sequence ID" value="CAD8210478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YB97"/>
    <dbReference type="OrthoDB" id="10252009at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000118">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF519">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE MPK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="5">
        <location>
            <begin position="12"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="6">
        <location>
            <begin position="76"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8210478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="271" mass="31400" checksum="CB018D2D6887CDF8" modified="2022-10-12" version="1">MYMHINEMDLILPSTKTNGALWLGNIKSAQNIINLSKENIRTVITVANNVNLSYPKHQKIIHKVFKIHDKENVTIQELIEMTNEEIEEAMKIGSVLVHCMAGISRSATCVIAYLMYQNKWVFEKTLKFVKSKRPCVNPNEGFKKQLISYSNEIQKKLLPQQQTQQIENPLAKLRRQLHQSPEDLIKNTQLAKSPRHEIKTLTKNSTEEEKQQYRQQLAQKLFVNTFSTTERSRQQLKTLLTNKSANIKGCISMYKQVEEQKLSGFLSDRLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YBR6</accession>
    <name>A0A8S1YBR6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1610025</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000161">
        <property type="protein sequence ID" value="CAD8210843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YBR6"/>
    <dbReference type="OrthoDB" id="282598at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8210843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="64" mass="7306" checksum="66ACC0C6988E3F79" modified="2022-10-12" version="1">MDIPLNTIILMNPNGTIQIGTQPWFKGKKIMKCYSCQSNIVFEGKYNQIKCTKCNTINQVPLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YCT3</accession>
    <name>A0A8S1YCT3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1630080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000163">
        <property type="protein sequence ID" value="CAD8211431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YCT3"/>
    <dbReference type="OrthoDB" id="299525at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8211431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="252" mass="29760" checksum="FEDA9069AFF3334A" modified="2022-10-12" version="1">MNQIDQKYSLPPLRMSEIQDLLLQSNEQHKKDIILSYRDTKKKKQKEIAELEKFKIIERKVFNSQSKLQGQTLGKNLDIFLCKEPMIDDYLKRHIIDPQRNLEKGLKDIKTGKNQLLDGRCFGDIPENKLSKKEKQSNEYWNQYNTQLSEQRYKILLDSQTGLQKKINQQNRLKALKIFVPNKYSPGYFDNHPSQILSYTEMHKHSQPQIWSPNSFYVDSFDDNLNKQLSKNNLKLSHTISKDQSKQSIKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YFK0</accession>
    <name>A0A8S1YFK0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1750039</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000175">
        <property type="protein sequence ID" value="CAD8213216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YFK0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8213216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="15039" checksum="F19841D4FD556147" modified="2022-10-12" version="1">MAEILVDEGLLRIFKIIIQFYGKVPLKYPASRSQRKAVILVQELSIKLVSFAILDTYYFLKTFRKGLKIISSNTKFPRFLQQSLTPKLQFLSFFCITNQKLKQFLFLIFLMMIDCFQQLFHLFIYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YH84</accession>
    <name>A0A8S1YH84_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EML-like second beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1890017</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000189">
        <property type="protein sequence ID" value="CAD8213896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YH84"/>
    <dbReference type="OrthoDB" id="309448at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="219"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="228"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="261"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="303"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="345"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="387"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="429"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="471"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="490"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="513"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="555"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="597"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="639"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="648"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="681"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="723"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="765"/>
            <end position="806"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8213896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="880" mass="97499" checksum="A580C8B5236FCF29" modified="2022-10-12" version="1">MYCTFQEKEALAEIFTQVKDVDEQIFDVILKIFRKEKVQDCIGYLLDLGNQTHLEQQILQKVENLMQVENGQQLYDVRKNIKQTTNVLKKIKDHGFYKLDYSNQVYEDFMQNLIKSCRNKIKIIKFLEFLVHLTSIDQNMIQCGSNSLYILVLIKVDLRNKNFLNIKIQNTSLVGANFARCNFSGSEFNNVNISGMNLNRAILMNCKWINLRIHELNKLNAHSDYVQSVCFSPDGNTLVSGSNDKSIRLWDVKTGLQKARLDGHNYTIFSVCFSPDGNTLASGSDDNSIVLWDVKTRKKIVKLDGHTNDVRSVCFSPSGNVLASGSWDNCILLWDIKTEQQKAQLDGHDSGVNSVCFSPDGNTLASGSDDKSIRLWDVKTGQQKAKLDGHNYTVFSVCFSPDGNKLVSGGNYGYIYQWDVITGQQQAKLDGHTNCVMSVCFSPDGNTLASGSDDNFILLWDIKTGQQKAQLDGHHSGVNSVCFSPDGNTLASGSNDKSIHLWDVITGKQQAKLDGHTFYVMSVCFSPDGNLLASGCCDSSILLWDVKTGQQKAQLDGHDSAVSSVCFSPDGNTLASGSDDMTIRLWDVQTGQQKAKLDGHNYTIFSVCFSPDGNTLVFGSDDNSIHLWDVITGKQQAKLDGHTNCVMSVCFSPDGNTLASGSNDNSIRLWDVKTGQQKAKLDGHTFYVKSVCFSPDGNLLASGCFDYSILLWDVKTGQQTAKLNGHNHCIQSVCFSPDGNTLVSGSHDKSIRLWDVKTGSQKGKLDGHFNRIMSVCFSPDGNTLASSSFDYSIHLWDIQNGNEISSADKNYKNILSQFIGPLFQENPLSLSNSITILRISQTALFQAQGALILKGNFVSYQGYNLRSLFKSKGSCILEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YJ32</accession>
    <name>A0A8S1YJ32_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1640041</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000164">
        <property type="protein sequence ID" value="CAD8211562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YJ32"/>
    <dbReference type="OrthoDB" id="342064at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031053">
        <property type="term" value="P:primary miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025239">
        <property type="entry name" value="DUF4187"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039727">
        <property type="entry name" value="SE/Ars2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021933">
        <property type="entry name" value="SERRATE/Ars2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13165">
        <property type="entry name" value="ARSENITE-RESISTANCE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13165:SF0">
        <property type="entry name" value="SERRATE RNA EFFECTOR MOLECULE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12066">
        <property type="entry name" value="SERRATE_Ars2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01173">
        <property type="entry name" value="DUF4187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="432"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8211562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="553" mass="66130" checksum="7BED4443CACC7AB5" modified="2022-10-12" version="1">MYVYNFIEVIILININGRTSILQKLFIGIIIGLKSKQRPKQKNKKKQTKRIKHTVEFPPLDQQLLTFDQFQELYVSNDIPTEKLRLLYQTYERKWENKQKELFFDQHKDEPWIQDKYNPVNRREFQEERNQLAQNRFNEFLKQYNMGSFNNINLTLTEMQLMDLFELNDHFYNDDPDVPFWEFQLKESIDITKAPFFGTDPNQNSVFLKNVPLPLSRADVVKALSQMAGYVSLTLSEPNKLQNYTRIGWVSFDGEEYCNRCVNDESITIKGHSLQFQKQQEKRKFIRVFKSVEYNKLLKDLQQQRELIKVLDKEKGISGNPLIEGPLKDRDDPTFNRQLDIQTLYLRRIHNYCYWSGAEFSDYRLLVSKVGYHFFRLDQKKKCNLEWQDIIQALCNRRREESSDELPLNELIDNEIIQMGLKQIKTRQKDAYPCIFCEKQFQGGTFLIKHISLKHEKQYWEHQQSLKQQVIDQQMANNHKIERISKATIQRQIFLKGVIEETQLQENKVQLPINFAPRVQQRQYKDLDSIAQDKTQPAIMFQMRETKVNYNDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YP15</accession>
    <name>A0A8S1YP15_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1720032</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000172">
        <property type="protein sequence ID" value="CAD8212944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YP15"/>
    <dbReference type="OrthoDB" id="10311287at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8212944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="88" mass="10471" checksum="A60A6DB46D04A3D4" modified="2022-10-12" version="1">MKSYKQVNYFQNYIKNSLNFWQQRLNNSSQTQTNILLKNKQRTIVDRVVIRSTKYWKQIADEFSAQSSTNKSVQSLSSQSHNQQIYDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YQA3</accession>
    <name>A0A8S1YQA3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1840012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000184">
        <property type="protein sequence ID" value="CAD8213694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YQA3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="211" mass="25508" checksum="700273624455B81F" modified="2022-10-12" version="1">MILLKMEKLENTLIKQFRRSQRGFLFGLHFSLQNYFSILQLCQLYQFMANQMVILLSSQYASLLIKLYYHLVVEITLSDYGDYIGQQQAKLNVHNSQLAKLLKKLRQKRKFNLKIIVLKIRCHKQNSTFIRHYLQSIYFLIIYTLRIYLFSLFPKEKYLSMRSINLERRIFQSLRSRFKIIFQIQRKLILRIAKLKGVNYQNINDQNILII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RXQ9</accession>
    <name>A0A8S1RXQ9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tubulin-tyrosine ligase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0010508</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RXQ9"/>
    <dbReference type="OrthoDB" id="288171at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070740">
        <property type="term" value="F:tubulin-glutamic acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241:SF147">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE TTLL7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8132232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="560" mass="66724" checksum="018EF383F6F51AEE" modified="2022-10-12" version="1">MIIYKFASAIIIFSFIYTIQVSICDYHDSIQKKLKTHYVPLLQTFMMDNTNYSKFKQAYNESSLNMIEEPSYCIESDTLKLLHPDILLEDKYVVRDLDKGNLQREIIKKRMFDSIQNLSPLSKPLTKFQHRIPPQLSYFYTFKPRLQDNYQLGQGLGCMFQKLNHIPGLYTLTSKNSLIYNYAKYMYDMKNRNVSQQCIQNASYVPHTLRLNVKSECQQFFKKLTQLQNDYKNNVKIYNGPEYLLKTDEHRGEGIRLLFQSQIENITEVYQNGEKCGNITEKIVTQKYINRPFLYKGHKIEFRIYIYLASSNPLQLYVYKRALIKRCANEYDPLSEQIPAHICNTAVTEKTHKNQTNQGDGNFEQEDEEMFIDWNLEGIEELLIEQGRISKKKRWLQDYLYPRIYSKIIHTIRSGQYSFYQDSRFSEFLAIDFLLDNELDIWLLEINYNPQILSVTPDRIKRNYKMVEDTMEIAFAYLKSKYKRIKQFIQQELYNYKHSGNRIRQVDFKNKFGNKFHQLLEDTSIDQQFSISSDNLYTHIIDESKNGKDKYFNLIEDECL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S4H0</accession>
    <name>A0A8S1S4H0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">60S ribosomal protein L34</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0040026</name>
        <name evidence="5" type="ORF">PPENT_87.1.T0080501</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL34 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8140198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S4H0"/>
    <dbReference type="OrthoDB" id="411713at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008195">
        <property type="entry name" value="Ribosomal_eL34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018065">
        <property type="entry name" value="Ribosomal_eL34_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10759">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01199">
        <property type="entry name" value="Ribosomal_L34e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01145">
        <property type="entry name" value="RIBOSOMAL_L34E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8135046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8140198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="12225" checksum="79391C3AE73F88E3" modified="2022-10-12" version="1">MGVRVTYRRRTSYNTRSNKIRKVKTPGGNVVVQYPNKKTSASTCADSNLNVVLNGLKRIRPTKLKQLSRRQRTVSRPYGGVLSAGALKNRIIRAFLVEEVKIVKQIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S7X6</accession>
    <name>A0A8S1S7X6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0050104</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S7X6"/>
    <dbReference type="OrthoDB" id="10452572at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8136346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="110" mass="13402" checksum="05BA5FD7AE2D198F" modified="2022-10-12" version="1">MIVNQIYQPVEFIYSYTIGGKQEQHLQLKNQLPQKKRNLTDEDRRKIKKEIQNEFNKKKILKKKIHKQIIIKVNHKKYLKLFNKLKARKNKVKKLSLGKKNKNHKERKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJ15</accession>
    <name>A0A8S1SJ15_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0070305</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJ15"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF69">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_38, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="2">
        <location>
            <begin position="76"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="77"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="128"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="2">
        <location>
            <begin position="136"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="2">
        <location>
            <begin position="179"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="179"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8138772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="245" mass="29732" checksum="87938C5646B32C17" modified="2022-10-12" version="1">MFQNIIQDSICSEKEISEQDLHSPHTMDLEPFSDFESNFHLSFFTEEPILECQTEVSQIALKIKEEDTNSNQQNQEINKRKQKIWNDEEDQRLKYLFNYYQGKWNEIVKNMPQRNASQCQQRWRRINPPKETKHIWTQQQDDQLKLLVQQFGRQWMKIAKFLGNITGKQARDRYINKLDQSINKEPWTYEEDMLVLDYFVNHGPKWTKISNLLVGRPENHVKNRFYSFIKRNYLGEQNRYQIIYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SK81</accession>
    <name>A0A8S1SK81_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0070482</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8139122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SK81"/>
    <dbReference type="OrthoDB" id="10264606at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="38"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="71"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8139122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="239" mass="27425" checksum="997CBA7D74D6C9BF" modified="2022-10-12" version="1">METFHNEQFLTYAQKGSLEEIKRAIVQGNVDINYQDKEGNTAMFLAIMHNHLEIVRFLIQNDASLEVYNAQGSGPLHLAAEKMNKEIVLLLVINQADPNLKNQSGQRPGDGITEIRTLINNLTAESKAFNVLKQSQKQKLQAIFEDIDYDNSKYIDHAKAVKFNKYIEDTITDNQAEKDAKDFIKSVALCNPERGVNIDEWLYSFSKLLVVDPAAFDKFIEDYDKQVEKKQKLRHQMQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SQ05</accession>
    <name>A0A8S1SQ05_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0090146</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SQ05"/>
    <dbReference type="OrthoDB" id="299052at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="609"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1023"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1026"/>
            <end position="1039"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8140574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1046" mass="122861" checksum="B4C5E00887DD468E" modified="2022-10-12" version="1">MWDQSFKLVPDQNIALVFDQALNANDDYLRLLKITEGIKYVTQKLPQATTFSSSKLLFQLMCLYPFQKSNIESLISAFFEYFQDFRFISSFFEASSDISMLQETSTQTLKQRITMLQRFNNKPKDGHDGQQFSQAVTQEIVTIKLFTVKYLKKFFELANNLQLDFQLYCLMFKHYIQNSTNQKLIENVMDCFQDLRFTIFAKCDWVNQFSEEIIIFFKNTPKLFPSYTKYKIVVIEEDSDTEIVSQTQITVTQQQNKRRSYVSSEQDPSLYIQNLCCKQDPEQPLQHNSVFEEIENIDILIQFVLDPIQISVASLWVENFPKLPDIIDNEKQIFKYKSTNNLSKYQVFRSFRALQILTNIENFKNLLGQYSQIFAKTFAFINITLYDETKNINLYHLAMMLKFLINLQPKFCIKQLIEQKIFPKLLEMSYNPSINQLLIEMINFNEDIIKLGIYYLDQLWSYLHQLNFLQLLSNYSFDTQLVVDTPTPDQTAYNDIFQVIKALSIFDPQRMQKKLLKQETQDNINSIQSKNDYDNLEAFQNEKQNKLKKAEDDLLRRQSSISMTQNNSKVHQIQKRKSNNFYLPKIQSNTSCQDGDGILINNSQSIDNKLNSGNIKTSPLMRRRNQTSQHQGKNKMKTEQDNDLYSNDGFSSSKLVQIYPTTKSVIFLSDFEKEKSQFNKNNISENHLKMLILLIDQLIVFAEKQQEMQKRLPKTQIFNINKFAELIITEKNIYSLFKVFLYSILEIPHNPTSLSVECGLLLHNLYIKVKTLQIFENFKELLKNQFQLIIDYLVKCIVQLNSIKQKLDSSSFAFKQYILVSLMTNGLLLYDRQNYMIKVDKNLKSVFRNLTDTMLHIIIIWFSDSQQNTIYQQIFTKFITIYFAGAPMPYLSNIIFKLGLVSSLYNAYTKFYQNEIKDTSYVEGIFFYILLINYIIRNAIKVRNLSDLQSALIILDSWQQLEQAQPFENPMALRNADQLINVEQLKQVEKVQPKKSEEKNLKLIKTKTQINQKVNSLMSLQKKISEPNTNPTVQTARPEQSQRKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SSQ4</accession>
    <name>A0A8S1SSQ4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0110408</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="292938at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035880931" evidence="3">
        <location>
            <begin position="18"/>
            <end position="2840"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2351"/>
            <end position="2371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2466"/>
            <end position="2487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2499"/>
            <end position="2523"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2548"/>
            <end position="2575"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2626"/>
            <end position="2645"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2657"/>
            <end position="2680"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2686"/>
            <end position="2711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2808"/>
            <end position="2840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2808"/>
            <end position="2817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2826"/>
            <end position="2840"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8142928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2840" mass="324536" checksum="AD814740155F2CBC" modified="2022-10-12" version="1">MNLLLLQFSNLVFIAFGQYSSQKVEIGCLKQLDTGSGCQELCVLGVEDCSTNPPKCEYGYFLFTLGSSDTKPPECVQCPYYEVNINSDLVNYCMDCFEDPYGWEFSRKCTYDYEVGNGKIGQGTYQKIERDYPELFFVGNSIKLGTWSETVTRSRQMKYAVYICTGCKDFCKTPDESSCYGFDHDYKKVDYDKIGIECIEGYYFKMNKCESCSIKYCSKCSSIIGSGITDNCEQCQDGYRWSSLTKSCVKCTNNCSLCITESEYNVEYQKCKACQSGYIPSLDLKTCEGCEANCLRCEYVSKTETTSIKYPSRSYLQVNDKDKYVKMCRQCQQSNDIINYDGQSCVPTQISNCDIGYYKITLKESAFMTRNSIDLDFTPYDRDTDLDPVSMCLYCKTDFVLSEDGTSCTAAIAAKKISNCIAHQFSGTNYICIKCSSGYSLDTKQGQCFLGCSDQIDNCAYCYSYDSPSDDIVTYYDESGTQKTGDTQKVYACLICDEGYYADFFTNSCLPCPSYCSTCSQYNKDYNFTSYIQQRVIVDSFKKSKYLTTMLEEPFCSLCTTGFSKYGNSCVGCTKNCVISNPSQVANNPLSFLLNACEYQRNGAFCGQCFNYYRDRSLNSELTECQTCPYRCTACRDRTQDELNRVNYYFSPSNSILLKYSRLCYAFDENIPYSYNVAYDTYLNMPIECFQLNLQTGKCVQNAQIDIQLYCQSDEYLSEYNNVLNADRKNYIYSLSYFYTTSTSSNYATNITLDSVETLTFYKSLNINVVTTLVLNVYLKSGKKECNFEKSTNFLFQFQRNAFSIRFFHVNIIGQTDSNQSPLVLYLDGILSIPWSDYLTIKGIEIKALTTNSTYPYTGLNISQNVKTKLSLENFKFTKIHSSTKMVFLAENIDKINWKKVEFSGLKSDDIFSLPYPLENIDTVSYIFDDVTFYQCNFENAILFSPNSNSNVKSIKQSIGTLGMTFQKCKLTNTVIFGEKEGSAINSGIINFGKVVFKFTTFTNSWFVNLKSQNKTIINNLDIIRCNFTEGSKLVQSNSFYLKNIYSLNSSFYTESRLFQNSYKVIDELQASIALQQYTFNNVKIIKSLCLTPVCFIKISTPINNYQYQTNISLSDFYIEQTNSMKLTDKESNSVFSALILLTSVQKIQISNFQSLDNLGITQIAVNKIQSFQLIDGVYDWSQSQYYNSQLNPTNNKIPTSKECADRKTEFKSYNEHFLYISDFEIGVMIENVVMRRLLSIDTNFIQIKSWDVEQFNTTSDVVNQDEGLFYHNTTREILKLKNIKVIGNSLVITESGTAIGSIYIQSEQDQDIIIEDCEFSHNHLHSLISDQLTPSSPVFIIQAPLSFLTIKNSIFNNNRATRVHNSVIYIVTYSIEIFNSKFENTNIRQKDWVYRLEEQQIVNSTSIQKDEDYIITILEQFFPIYSQGGNMYIKSDTTKIENSLFDKGQAIRGSGLVIETIDSGIISIINTQFTRQYNSLLNQYSSNGGCLYVDSSNSKLQLNIDQVIMSECVTRLDGGCLYLDPSTYQSSVTISNSKFNNCMGLKTSALYMPFSTSSPILPSVSITSSSFSQNNMINFLRNIPDLTSIDNEYLYTKSSILYQSYGRFTLSSSIFKEFNYVAVMNLEEMVEVEINDVTVTNNVIFINSIISITLYEISGVITLTSTKILSHSQYDPPSSNKCVNINDYYVNYESKNILYEQTCSAVDFLDSNFRSDGLPTIINQIGDNNYPIYFSSLDDFYKALTDVLKYKVDKSNYNLQFQHTILDNIRLNLDFPIFITFPNEENSIFIGCYLSTLLEGYTDLFSSITTGLFKLNSVTSNQYLTIEQITVENNKCLRCKNGLIQVLKIHQETYPVIEINDFLCLSNHAGYYGCLVLTYDNELSEVEQSIPLTDYELSNSRRILSVVDDLLKQKNVIIISRSQFIGNSAKVGGGLTIIGLDALIIHSEFTDNYASLMGGALYYKSVVYKTGSTILNIADNTVEGNVAQLAGGMFISGGSIGNVSSIEVFFQNNEGILRGDNLEEYPQSLTLSMKSNELMKTITFEDYDGDVNEDGFIDSSKSTIIDIISLDPYQLFNYKNTTTYLLLPSGQAINTYKYYYSDTDEQVPYDWQFRIINLDRYKQPMSNIHSTESCTLTGRIMDIDNYNTSKEFLSNFTNQQIFYYDQKAGAFDLDSLIVTFDPYLEDNLYLQLRFECTTIKIPVYETVSPFKILSYNQNYALYVNVKTLPCQLGEAYQLQKCKACTKSDKTYSVNEKQTQCQAMDSTTMDDVSPVGIKLKEGYWRPEFDSEIITYCLNLPQNCNGGWKPGNPSCYTGHMGALCESCDIYGVRDEFYSTSAKYKCGPCADTLTVNAIVITALSFVTLFSMILSVKGNYQMLEDFVKTQNRIAMGILVSPSQSNLAILIKLFTNYFQILSAISTFQINLSEGITNATQVMGNPTQSMAYSLDCFLVTMTSIEILYFRLIWALLMPIIYLAVFLFGYFLAVLLKKVDFKEGIIYTAFIYMFLYLQPTLVGGFIALASSREIGGFGWVQADVLYQYDTDTHYIWLAGFVAPMLICWAFLFPCIFMYLVYKLRNKLDDFDSRKKLGYFYNEYTKEGYLWEFVKIFEKELIIIFLTFYEDRVVVKGLIIFLIVFFYGGFTIKFQPYSSKRLNFIDRLSTAICAASLCLGVLIYSAIKEDLVYLEIIMIIIVGLINFAFILLMVYYLFEGYLIKFQPQLDKIRDELRIHHPEIQEKYPWSRKMLFNQAKMNEKVKKLWATLRYQTRRGIQRRRKDPQAPLFVNENSDQPVLMPFFVPEEIKQQKTINQQDSPNYQKIYPEQDGLQSEEINLNQPNQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SVB8</accession>
    <name>A0A8S1SVB8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2 tensin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0110255</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SVB8"/>
    <dbReference type="OrthoDB" id="302229at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014020">
        <property type="entry name" value="Tensin_C2-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051484">
        <property type="entry name" value="Tensin_PTEN_phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45734:SF10">
        <property type="entry name" value="BLISTERY, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45734">
        <property type="entry name" value="TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10409">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01326">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51182">
        <property type="entry name" value="C2_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 tensin-type" evidence="2">
        <location>
            <begin position="143"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="327"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="356"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="368"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8142624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="380" mass="45320" checksum="0852BD0775A3C012" modified="2022-10-12" version="1">MPFPKQEYILQVSQYLNRKYGHHYYIWNLSEHDYTRELFQNQVSMHSYIGFACPPLYELLLICKCILEWIQHEGNIAIVHCQQNKGRSAILLSIFWCLVFKTSIEESFYIIAKALGLTSPLKSQLMYIHKYLQHLLSNGQLNTQIIKVKSVILSGIPKKFENFKPLFQVLDRKGILYEEKNQIIQKNEQCIFILPNLQISNDIVFRCKHITPENDLIPIFRFQIHTGFLFKNIIRFQVNDLDFQQSFDDDIYIDLVYLRGNENNNLHDSDKLNYDQQSLEGYQMIKQLLNTCQMMSLNIKFQLFQENDIFNQIQQNQQTIQQIQKFEQQEEPSEKNQRKNSQQEKENQQVIQNPIKQEDIYEKKEIENNDDDDDDDEIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SXN4</accession>
    <name>A0A8S1SXN4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0130015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SXN4"/>
    <dbReference type="OrthoDB" id="298777at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8144089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="534" mass="62084" checksum="0AA835E86803F7EE" modified="2022-10-12" version="1">MGNACSNNKSFQNKKDKISQQSAQQQKTKNGQNENQNIIQEYECTYWHSNQHRWIQKNCKITLSRQKEIIYIIDGSIHRIEKLLDSLQRPEVFTNFEQIQYLQWYGNYDQNLKKVGKWTAYWKGKYLSQVGGYYSEDGSKQGIWKEIMKQFCGKCQVFELGKYQNNQKSGLWIYFYGNKKIGGGFYNEQAQKKGQWIELSENFNDEAQVMYKGEYKNGQKLGIWNILDNNRNMQKYNIQFNNNFCVCVFSGGGLYDEGSDGVKLGLWVEILNGFNNYSQVIYKGRYQNGKKVGRWDVQFQDVQENKQIGGGSYDEGGHEIKWGNWVEILDGFCCNCQVTHNGEYKNGNKIGKWDIWLLDSQENQKIGGGLYDEGGVGIKVDNWVELLDGNRCIAQVTQKGQYKNGKKVGEWDILYENQKIGGGQYDEGCNGIKIGRWIDLDDEFYYNKQVIYKGEYDYGKKIGRWDIEYRNDSNSQFLKIGGGLFDESGNELKIGKWIDLEEGFYYFKLITCIGKYYNGKKLAHGCLWNKNGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T1Q7</accession>
    <name>A0A8S1T1Q7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0140223</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T1Q7"/>
    <dbReference type="OrthoDB" id="285980at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8145399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="143" mass="16779" checksum="9A2C922803C2069C" modified="2022-10-12" version="1">MNKVDIIKKFSLEYSDEFLKRIEHQSLQQIIKLIFESPLAKITKPIDLKNLKQLNKPTLFEISAVQNISEPKKTRYMNTKDCTLQFIFYPNIVAISLQKHPELDQDLFQLEGKKILIPQGTEICRSILILKQFTLINDYNQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T2W2</accession>
    <name>A0A8S1T2W2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0150153</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T2W2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8146058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="69" mass="8415" checksum="2CDB11DB0F312159" modified="2022-10-12" version="1">MNNERIHIDYLYKPLYEYTKFTELQNHLSYLISKKILKQFSLPNSNINIGPPIKYEKMIKYNKFQKLCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T423</accession>
    <name>A0A8S1T423_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0150290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8146329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="192" mass="22864" checksum="DCC9FEBD53F66C0C" modified="2022-10-12" version="1">MEQPNYKSVAEYQAHLYQKNVAYQIEQFLCQKLGQEAELNLQCTFSRIPVTIPIRHQNVASIKCIFELELWLQFFESKLKNQNNFSCPGCKQYVYFQDFGIDFTLYHILTAKKKLEQGGQKLLSEKVIYTFNNGKTXINQFELQNILYKQNPQSIFLLIFVKVDIIIINYDNILFIFSFFNIFLFSFIFKYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T4R3</accession>
    <name>A0A8S1T4R3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0150035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8145834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T4R3"/>
    <dbReference type="OrthoDB" id="307841at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="11"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="161"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8145834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="216" mass="25641" checksum="999DB775660927C2" modified="2022-10-12" version="1">MSESRAFANAMAALQKKSAILEEENKILHSQLKSCQYELQKLVEELSNKDNIFETLEKKLKITITQLEEELFKKDEEILQKDAQIESLSQELHQSLIDQSRIMDQQLSDRQQLSEQQEQIKKLKQVIHKLKEPRKDDVRFTQSEKKTSFVSKLDNAVEQYFNNENQGYEQLIRRISKLEKEIKDMENEQSNKILLDQKIQLLKELQEQEESLLQYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T5X3</accession>
    <name>A0A8S1T5X3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Spt4/RpoE2 zinc finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0180184</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T5X3"/>
    <dbReference type="OrthoDB" id="248751at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032044">
        <property type="term" value="C:DSIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140673">
        <property type="term" value="P:transcription elongation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.210:FF:000014">
        <property type="entry name" value="Transcription initiation protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009287">
        <property type="entry name" value="Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022800">
        <property type="entry name" value="Spt4/RpoE2_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12882">
        <property type="entry name" value="SUPPRESSOR OF TY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12882:SF1">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06093">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01389">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="Spt4/RpoE2 zinc finger" evidence="2">
        <location>
            <begin position="24"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8148675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="93" mass="10542" checksum="3FCFE92AF6647EC0" modified="2022-10-12" version="1">MQSESEMSEYDEKKFILPKSTKDLKACQQCGMVLTVEQWNREVECPNSCNASQTKLFSGMICVLKPSQSWVMRKLGNPKNIHPGLYAIDVQAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TEQ0</accession>
    <name>A0A8S1TEQ0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0200057</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TEQ0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8150034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="211" mass="25658" checksum="6DBC90E33CFCC7B0" modified="2022-10-12" version="1">MSDFRDFDIKQLHDNIQSCFQYLDKRIYRAMNVAPISNTIIIEKPNTAIAHEYKELQNHVKQLQLKYDEVCYKYMQLLTNYQKIKITLKLQEEEIEQQCELIKEMKLIRLQQPQVIQIPVYEYLREFTRNNHSQLSNFLQILMHTKKMDLSMIELLQNIEKYYRPQNKQLFQDKYIYPKVPLKKIRERILKTLPDQIVSQNLIPERSISFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TIN2</accession>
    <name>A0A8S1TIN2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0240001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TIN2"/>
    <dbReference type="OrthoDB" id="291224at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="126"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8153045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="481" mass="56466" checksum="FD74E384CE50BE03" modified="2022-10-12" version="1">MKKTTTLQSDYSKSSFFNDGKQIFCNSPHLSMEFTQFGAFIMKYNNRQQPVTIFLHEFIMKIVEEKGERHINLENICVKKTLHQKTQQLGLRLSKNGQFIEFFGEIMELWKIIKKFVIQTDFQLKYKLGQKLGEGYSSSVFKAFKIINGQKVAVKIIDKSLVQRNNASERQYLINEIAVLRQINHNNLLKLHEIFECDDNIYIVSELLEGGNLKNSLFKFRFSEQETIILMETLFQSLNYLHERNIFHQDIKFDNILLKDQQDLTSACIINFGKSEIISQKLFSSKNLNCSDQTTVTQYFRKKDILALGIVIYSIFSKKQYQETQMLDELLMKNYENMGDLEFLELKPSLLQFFQIFFTQKGRIQMDSIKCSDILNLDVFNLTNTRRTSKIQGVPHQINNDFRRTSRFAPRNSQKDHQLITNFQRNLKLPTIQDSLDYKINQNSSYSPQTEKCISSRIYDEQASSLRPRYSLLKKCLSPNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TJ58</accession>
    <name>A0A8S1TJ58_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0220170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8151913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TJ58"/>
    <dbReference type="OrthoDB" id="305514at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038835">
        <property type="entry name" value="Giardin_beta-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37027:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_148, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37027">
        <property type="entry name" value="KDE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="107"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8151913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="249" mass="30149" checksum="A3D5DEFEA40D6644" modified="2022-10-12" version="1">MTDQLEERAQRIFGKLQSLQNNVEGEKQSKFKLILGYLQSVELAIEDVQQQKNQKFQDMAEKLQRLKSIVDSEKEARDQLDEKMRKEILQVEKNSKNLLNSFSKERLETEKRVFQNLSQQIDQLSIEIQKEYHQKSETQNRLLEIQNEQIPLFYQDIEQEIRIREEIEDKIAQQFNEQLNELKRIFDVQQKQREQREEEIIAILKKIYQNAYDVCKRNRTERERNEDLFVKLVEQVVEKIKREIIDSDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TKX4</accession>
    <name>A0A8S1TKX4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sphingomyelin phosphodiesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0220179</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Converts sphingomyelin to ceramide.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the acid sphingomyelinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8151922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TKX4"/>
    <dbReference type="OrthoDB" id="282973at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00842">
        <property type="entry name" value="MPP_ASMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000221">
        <property type="entry name" value="Sphingomyelin phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045473">
        <property type="entry name" value="ASM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041805">
        <property type="entry name" value="ASMase/PPN1_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011160">
        <property type="entry name" value="Sphingomy_PDE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10340:SF57">
        <property type="entry name" value="METALLOPHOS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10340">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19272">
        <property type="entry name" value="ASMase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000948">
        <property type="entry name" value="Sphingomy_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035739356" description="Sphingomyelin phosphodiesterase" evidence="8">
        <location>
            <begin position="20"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="9">
        <location>
            <begin position="164"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Sphingomyelin phosphodiesterase C-terminal" evidence="10">
        <location>
            <begin position="440"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF19272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8151922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="605" mass="70535" checksum="0F0543EC982C6293" modified="2022-10-12" version="1">MRNLLVIFALLCFVANTIDMGEELMKREILTKIVEHAGIEMPHDLHLLCLPCKLMMKQVQKFSSTILLSIIRKEWTLLCPKFKDPAFCEGINQTFIDQFSVHFFNNYLAPSNACQNLGACKLQNEPQTIKDYINEVMQDKLSKEEQKQWQVMAEENIKNQEDYTIAQFADLHIDVEYSVGANAFCGAPFCCREENGKPKDPSKGAQYWGTYAECDLPFRTIQDLIKFTGEKIKPDFIIWTGDSTSHDVWHQQKWNQTLPTKMITDEIKKQIPNSQLYAIYGNHEGYPADQYDMIGESTQWLRDEVADMWKQYLTQESYYQLRRNGYYSQVDEARNLKFIALNSQACDLLNFYLMDGITDPRGMLKWLISELKDSEAKHQFAVIFAHIPPGDTFCNSQWADRFSVVIERFEHVVTGIFYGHTHQDHVQHIRSKIDGRYVKTLFIAPSGTTFSYQNPSFRVFQFNGKNNSVKDYVQYRLDLAKANKDGQNAILNWEVAYNFLEYYGLESASLVHVSSLPYRLKHDDELLKKYIYSYSTGSDVLYKKHLKDLKNLFLKKSTKSFFICGAETSTLDDWYECIGFFEKFKESSFLTFKLLEQFIGKWLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TL65</accession>
    <name>A0A8S1TL65_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0240341</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TL65"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8153665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="195" mass="22243" checksum="D1A2044CBEB3F816" modified="2022-10-12" version="1">MTSGGNHILEIQDISKTAFDTTPEFFISLHYYDWRLELPHRLLVEVTLITRTVAASQLFAIRIQLFAFQDSRISVITFQQNNVYELQTGIGDRTIAFSKNQEFKDATNGSISLNCVKHIKSNRIVEVKIEQVQSQSIDFSVSIYQDSQIEYVRFNILLGTDQSLGSSSVQAEIFNPPQYGNVYGNYDIQADKQLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TL79</accession>
    <name>A0A8S1TL79_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0240348</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TL79"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8153675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="73" mass="8651" checksum="4B212F010837BE58" modified="2022-10-12" version="1">MAAKKQRPTQGQMMSTQGDVQSEKQMKLAVEYFYSKAYIKIRYINSIKHMDSEVQQKCSCFLFILNDQFIYIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TSP9</accession>
    <name>A0A8S1TSP9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Spindle assembly abnormal protein 6 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0290202</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TSP9"/>
    <dbReference type="OrthoDB" id="49058at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10142">
        <property type="entry name" value="HD_SAS6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032396">
        <property type="entry name" value="SAS-6_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44281">
        <property type="entry name" value="SPINDLE ASSEMBLY ABNORMAL PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44281:SF2">
        <property type="entry name" value="SPINDLE ASSEMBLY ABNORMAL PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16531">
        <property type="entry name" value="SAS-6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spindle assembly abnormal protein 6 N-terminal" evidence="2">
        <location>
            <begin position="63"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="209"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="292"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8156851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="595" mass="70311" checksum="9E17B1C3430F6704" modified="2022-10-12" version="1">MQDYRGNFKRIEFGIQQTPAKSTTNMNDFQSSQKLKYDQMSNKPQSSNISFINAPITTVSKELFTLQIPVKVKQMTDDWNTQLILKMIMQQSQQQAHGQILRIELTDDNNLQFLQILDLTESEFLVLKNEQSLHVDFATFPMKLADLLQLCINSQRDEKVNFYVCLETKNGESTLAVIENNEFKKLTHLSLKLRSATDDVIKVFLSNKLAIEKQDNEEKQKRNKKLTELLEEKHWELENLKSEIRKFTEDKDSAIQQLLLDEQKKFNDFRELTLSKETNFKRESENEKQFIIEKYDKIVLELQNKHAQLQQINSELSEQRSLLIQQERELKNKLTILQQENLQYQKENQEFRQQNKELDTLKFTQERQLIELRIQKEGFEKLIRDKEEYLNNKQQLVDNEKKQNTILEEQVINQKKQIEKLESRINVMSEEVNKGNQIIEKLENELSKQKDKIKLKNTVVLQQEQTVQQLQDANDLNHKQINELRREKDSLEIKLKDQENMNLDLKNKLSESQKLLESNNQMIQYLNKCLNETKVQVPAQMPGISSLKSQTFTKYTSPIPLQEDRSFRNQSVTNQQNQNNISQISNATISNKFNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TUD1</accession>
    <name>A0A8S1TUD1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0260042</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8154586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TUD1"/>
    <dbReference type="OrthoDB" id="304005at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="283"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="336"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="403"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="341"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="356"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8154586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="645" mass="76693" checksum="7F45E382DABA413E" modified="2022-10-12" version="1">MKENKNRNLQSQLSQQSLRSYEDFIRPIEDLSSTNLPSNQLHNFHTTSEINQNLSTHQNRQSQIITSYNSISLHQNRKNVNKNNDLRLMKEFIKKNKKYQTELEDKIFNSSQKQEGEVFDETKYNQNFNQINQSQDLSDDLDLLPSLTQRDQKAYNPQIQESEIHNSIMPESISNNTTARTLRNTSNYNSVHPKKQKYEHQEQFTNDKHEQLQTINEIQRKISPSLIRQSSIKNDPKFTENQQQQKQLKDFQGDNKVFNQLHSQIPEIIVHQEQKHDEQINNNYSQNSIDLPSQRTKNTQGINEKSKSSLKSSINFSDNNKQIIKDHQKVELINKSQDNQQENKRNKNKFVKQEALDESMEDESEYDSEDSEVEQFSNFHIKKQNKKKKAKVQSLSTSKLERMEDHKRYLQFLKEELKEMLDKKVNLKEIKEHINIILSEIGGLCLDQELDYYIQLYKSRVQELNQVNQESHEIRFQNQQPVCELRLKREIKGLKFAKECDRITKLKPTDIEEIIDTQRLIKIEEELNDQDVPTYQYSPQYRYESRQSPLQTNIIQVSLDVTRSQTPNKKRIYRTNPRFESELTNYNQRKKTPIRNIKKQKQSKHIQHITIKKDPLNDNFPYLNKYKPELLKSILEARISLEQLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TWV0</accession>
    <name>A0A8S1TWV0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0290057</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TWV0"/>
    <dbReference type="OrthoDB" id="305893at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="186"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8156564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="379" mass="45252" checksum="C5BF794A6A03CF99" modified="2022-10-12" version="1">MKFSSKSPTKTVKQQASISPSQVQKKVTHNYVEKTLVNKEVEYWKFKYMELEQFCQGKQSYEKLENELQIWKSKYQQLTLRARVAKGSELIIQEYQSRIQELNQQFNSNNYNSNVTFSPSKRSEQTKQNELQNKIDQQQNEIKSLQNQIEELKQQSKQHTKRDIITTTNNSNYQSQNMFANSSSLNQQYIQQTMRLELELKNIELENQSLQEQIQFYQNNLDNQSKIIDQQKLHINQLEQNLASEKLKQLKLMDQNAINLKEQEIKKYQEEIFILSSKLRECEKLKRQELNQKDEHYQEIISQLKLQIHNQIQSGEMRLSTLQNILQDKQLNSSFGRINNDFYQQQNTNQSDKVIVRLDSEMKNLKSLINEDNNKQRFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TXG3</accession>
    <name>A0A8S1TXG3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0290228</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TXG3"/>
    <dbReference type="OrthoDB" id="435593at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051345">
        <property type="entry name" value="Importin_beta-like_NTR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363:SF33">
        <property type="entry name" value="IMPORTIN-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363">
        <property type="entry name" value="TRANSPORTIN 3 AND IMPORTIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="808"/>
            <end position="832"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8156880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="918" mass="107895" checksum="472DB83D9D862C6E" modified="2022-10-12" version="1">MFSIEEFIKQYQNLSNPDKNISQEANKYILKLQQTREAFTVAQQILDQQNIQQEYQFIACQLIYRRLKEECDITIQPYLLNLLGRPLSNIALNQVCSSFAVIIVGNSQLWKGILQELIQLMKVKMPIGIEILNQIAIQSKECHSKKEQQKLQLEFQTQESILSEIFLSLLCVQDLNIFKQTISCIESWVQFSYRLFKDSKLIQQIIVLIQHALQNNALEYSDKLFSLLYEAVVYSQYNKNQITEDIVQQNLILMLEFILKIYNPQISVMFASFATKFICDFYPIIQNTQYEEPILNMMVNIVNNPQRKIVFQTFEFWSQMRQNNLIQGVGLKILQCLIDRCKVKSIKLTKQSLLGESEDEDDYFNKTDYESKDNNISTSDFREHCKEIFINIYKNVEQINQTNSFFQIILSNLVITNPESIEQIIKSEAALFCLCSIIDETDFQINNQLVLQIIQFVLQLPNNQNYDIIVKTTLQMFSGLTNQINLNNELLLSITKYFFQYMLHPLLGSLAGVAFEQISNNSELINQQLISDCVLFLEQHFNDFINQSQLNSFVEGILKLCYRIYSKGNIDCIIQLFNFANNQFQLLKNINLLTKQQFTYINTLIIAILTWINSNIEEINAQVKQISDLLCSSISEPLINLFKESQNLSNNDELYQLVRMIMKVSNYQNHSQSIIQLLQISFQIFYQNPTQKYQWLQLITIAIGRSQEYQFISQWTIQNSQQIHQFCIEQYKRWRDSDLMKIYVEFVKECTRYCQQALFQSPYLFLIMEIISEAFLTLNSYEMQREILNFFKTLSQFIEKGQDYFNQIVIQIITTLFLSISIINRAIIFQIIQILQFILKKEISSDELKELIYQTLLKSWGANKSPQQVQLVSQAILHYLKKTGDNGLNRELKLLLQNLREMGEEEIDYMHLEIVIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U0C3</accession>
    <name>A0A8S1U0C3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0300119</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000030">
        <property type="protein sequence ID" value="CAD8157373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U0C3"/>
    <dbReference type="OrthoDB" id="303940at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="229"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="86"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="164"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="283"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="397"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="249"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8157373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="585" mass="70441" checksum="2BCA7A920B4547A4" modified="2022-10-12" version="1">MMPKLSRENTISNASCLPRHLNIQGFQKFNIKSNSPRTFQACQELGIDPIVLELKEESDFKQNDLDEEIIQLRYQHYLNKAHQLFTEITQRRKEIIQRQRQKLQNNNASIESLDRSKTIKLQQSLDQSEVRRQIDSIYNRTVSFLHQKSESPTKDQFAGDVYDLNQLEGGIEKEIDRYNKHKKQKMREAQLKLQEDAKRLQLIEKWKQKDSQMLDQILQRQYAFKQKIKASSNGRKTPLQKIPQFTDPKSFKRDSDTKIDKGDTSLQKTMYRTRIDDSKIKQRREMVIQRKEVLNKLEDMELQQDFEKLQLKLNQSEKLSKQQAQIKVERIKEQYYREQQLIQQQKEINVVQSQEHLSAMISKMINKEQEFRGQLQSQLMSELEKKQLLKEKQKKIKQNQNDLYKDHDNKLKQLNDKFIKIEEQNKLRKQEQEHKILLKQELRRLKEQDKLDNYERQKRQNDYKMMTLYMKSKFIEEKNQLKQYQNEVIQKTSMEINKQEIQERQRIYSQLQDLSDNLLNYKSVSQHESRQKQDQAKFKSVKLLKKFAKLEDPNIEQHTKVLLTMLQPKPIEKDNSNRKLSVLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U0K9</accession>
    <name>A0A8S1U0K9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0310030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8157844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U0K9"/>
    <dbReference type="OrthoDB" id="295331at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8157844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="75" mass="8639" checksum="559F4AD35D5BEF70" modified="2022-10-12" version="1">MSELEAQCIRMVKFGQSNIEKTIQFFDNKLLDQIINDYSKMEQNLLQTSNNLNKSLIEAESTNTKLKQLVSLVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U179</accession>
    <name>A0A8S1U179_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PhoD-like phosphatase metallophosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0300121</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000030">
        <property type="protein sequence ID" value="CAD8157377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U179"/>
    <dbReference type="OrthoDB" id="2100241at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07389">
        <property type="entry name" value="MPP_PhoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018946">
        <property type="entry name" value="PhoD-like_MPP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33987">
        <property type="entry name" value="CALCINEURIN-LIKE METALLO-PHOSPHOESTERASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33987:SF1">
        <property type="entry name" value="CALCINEURIN-LIKE METALLO-PHOSPHOESTERASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09423">
        <property type="entry name" value="PhoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="482"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="PhoD-like phosphatase metallophosphatase" evidence="2">
        <location>
            <begin position="171"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8157377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="520" mass="60950" checksum="AD0FD09A77DF54F9" modified="2022-10-12" version="1">MITALKYEDSILFHWKGNLTSDSITMKVFTRQESFVKFRIISWDLNNIQNFSQLNVNQSNDSPITLQFQNLQPNTQYFYIFEFYDIENITSEYLMTVYNKEKDYQFTTEFDQPMFSFKTFGLIGQPYTFNFSASGCARSASISKIFLDIYQNTYPQFFFMLGDLHYRDINTDSIDEFDQAYRQVFGSSTQRYLYQQVPIEYQFDDHDFGKNDADGTSPTRNVANLLYWKYVPHGNLHNYLPLYKDEELTKYPQEVKHIDLINEENIGSYRYQIIGRTILITVDLRTFQNLEVLGDQFGNFSNERSILGKQQRQWLLAILDRVIITDWIQAVIFISSVNFHGEIWDVFPNEQLLIADKLQYIQEVLEKQVLIVSSDSHMTAMKNGQNSFIQSLGLKYKYGLQETMCGSLDKSGSCKTQPLTFGPWLGKNHYTNIEIQDTHLRTCIIVRAFEKTSQLFFYNTCDDRLKGLEGKVKCPFDVLMRVVYVIASLLVSFGFLIIIYIIVKKCHKNSMKYSLVMREK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U8T9</accession>
    <name>A0A8S1U8T9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SF-assemblin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0360050</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U8T9"/>
    <dbReference type="OrthoDB" id="310069at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038835">
        <property type="entry name" value="Giardin_beta-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37027:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_148, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37027">
        <property type="entry name" value="KDE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="175"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8161198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="279" mass="32812" checksum="88C9D7BB1FB48A68" modified="2022-10-12" version="1">MLLSTSKLTTSKLSRSQLSSPQRSTVNYLSQDLDSPSRQRIKLLSDKWNHIQNGIDKDKLEKREVLDERIKIIEDVLSQEKPKDEQRFKVLKDQVLKLQDHAHNQKSEREAFDDKKEKDFRILSDNVALSFEQEKNARGSAETKLQKQIDERFAQITLTITRNTHQYEDRSQAKIAEVLQQIQVVKNQLDQERQSREESAESLSEQIDSEINKFSDQLLIEKKVREETQGKIFRMIEDVHGKLQQDINFERREREATTEALLKLLEDACIKIDKNFRSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U8W1</accession>
    <name>A0A8S1U8W1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tubulin-tyrosine ligase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0350205</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U8W1"/>
    <dbReference type="OrthoDB" id="196367at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_125, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069">
        <property type="entry name" value="TUBULIN TYROSINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8160854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="719" mass="84127" checksum="44B689B236F46539" modified="2022-10-12" version="1">MRARGPTKEQESIGKSVDNNSVHKRRQELWEKQMGSNQVCQGYVSNTQGSAESGIKQFLSSMRKNGSFQQIESQFRPNITENETESPNKSQLKVTDTQLLTPLQKHTQKNQTTYDILSQIYELQNRKSRNQSLLEELMRKTKQANDNLTFQRILQRKKYYKRSQRCQSVAQKEYELEQLKGQLIQVKQVQVQQIQKEQTPKQQDKLVPLLIRKEKTKSPPSKDNERKKVQVPICKAKKIVIDDTRFWDIGFTKFIQNPSYQEINQLINFCNGIQVIPALHQKYYKLFVGRGNNHMMIKGIFNLRPQWSIANSIDDDTEINFVWTQKFIDLQPSEIKPIPQTIISEEINSWIDSQQMSLIKQAWDKVEGKSKKKLNEYNIDSPPILNNLQNQKELTQLNHQNMNIRIHNHLKGGNQLGDKKWLFHNFSEYCSEQSIDIWNYIPLTYHIHGPTDREFINFQQTFQHLALDKDIKNIWIIKPGEDSNRGNGIKVSNQMSEIVQHISQQGHTFILQKYIENPFLYQKRKFDIRGYCLITIMNGIKRVYWYKKGYLRTSSSFFTLESLDNQKIHLTNDAIQNKLNGYGKFEKGNKVSYDQFQAYLIEHNKQNNTSYSFDELYADMKALTKLATQSAIDKITNDDQILGFELYGLDFMISNSFKPLLIEFNTNPCIETGCPVLTKIITGLLENLMRFIIDPLFPAKRMGNDDFMNKNDFELLLSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UBH3</accession>
    <name>A0A8S1UBH3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0360206</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UBH3"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF271">
        <property type="entry name" value="MYB DNA BINDING PROTEIN_ TRANSCRIPTION FACTOR-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="54"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="114"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="2">
        <location>
            <begin position="117"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="2">
        <location>
            <begin position="172"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="172"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8161477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="306" mass="37089" checksum="5DB1768AA9D33AAF" modified="2022-10-12" version="1">MQKKTPLDRQQVLEMFFQPNYEFAQSLLKSHQFSFQQLPTVEEINSILNSVEKAYNSKRGTWTPCQDKFLNLLVLGTCLKNKEIPIELSSYQWEQISRMFRYHNWKACRNRWLEECHQKVSWTPSEDQVLIQLQKLHPNKWCEIAIEMMRICKTPYIRQGKQCRDRWVNKLDPNIVNLPWTKEEELKLFREIEKRGKRWAEISLKVFKLKRTENTIKNRYYNLLKQEENKIKLSRVTKDEKNQILVKSIIQQLEQQVNCKFPIKNEETVEQENQQYNFQIFDCNQIEEPLQLICFVKRKIVKLNEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UCD9</accession>
    <name>A0A8S1UCD9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0380098</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UCD9"/>
    <dbReference type="OrthoDB" id="301946at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8162538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="308" mass="36151" checksum="790259E1C4108289" modified="2022-10-12" version="1">MKTQRKSLIKEIKAHCPIVLNEPVGVVQPLCNKLYTKPLSDWKNVEAFIKWRDMKCQKSLALLYSKKQNCRIDLPIPQVKKQQDIHSTKSIDNKNKSREIIQHCQMNKTKKYEYVDTSLNKMYQIQDKSAQEYQSYLSKHLEDSRAMNKAISFNLQSQSRFQLFQKLRNMNKNILNSLSQQHDQIQSKQDDSKIIKIETTLRSSDEQSNLETSPITQCEDGQNNRIKNKLKSLIGPIQQNQITKNKQLTLPYFSRNQLNIKYSHFFVTNQDCENNQSSDNNSNINSLIRNKSSMLSREMKRIRIQTEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UHQ5</accession>
    <name>A0A8S1UHQ5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0370253</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8162206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UHQ5"/>
    <dbReference type="OrthoDB" id="313387at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8162206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="381" mass="44971" checksum="CE52E95AFB4F56C5" modified="2022-10-12" version="1">MAQMNKQNQTYIVNDYSRSILHGYHIDENREKKGGISQSSELGGGIFNSAEVEQRKPKQVLQKEYDSRSIRYANEDYSYLANLLLSRFSQLDTAASIATQYPLFKMLRGVTKKFMFNELEIIFFLHTIEDQKWRYDDQLISDFQPYFKQDFLSNQENQNVEGFKKLLLFLICCGYTIKCFFNDSNDQEIILITDHIQQYCQKDFKKSLLELWRQKYMNCSLKIVPRVLNKLYNKLIRIPKDGKQEFQQDYNALVDQIIQISPAYNSQEGKFIKQEVKPIQQQQQQYQQPQPTITFSQQTQQSYQQQVQMNNSGIYMQYSQSQMAPQQTQQLFIQTQASFNNQDFLDSNINENLFLNSTKIKEEMLPPPPPLLTQSSNYKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UIA8</accession>
    <name>A0A8S1UIA8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0390238</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UIA8"/>
    <dbReference type="OrthoDB" id="307563at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="124"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8163472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="227" mass="27154" checksum="DDC89D00980F62B3" modified="2022-10-12" version="1">MMYHSKGLKLSIIRKTILFNLRNNYFIELKINVIQSFYKISSSQFSIYQISYIYSQPFANKYLQANSFVKEQLKGVKVKQTKIEKHDFMKTLKMIEDQLLQSIKIDKKASCGEIQLKKTNREVELKQQKQIIQLQQKLSQAQKDCQDLKEQNHQIYTEMKKQKQIIKSLEREKQQYSKDKVIWDKKLQNQEELYSTNLRMTDDVKKVLNMINEDIDREAVVKAKKMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UL60</accession>
    <name>A0A8S1UL60_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Beta/gamma crystallin 'Greek key' domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0420064</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UL60"/>
    <dbReference type="OrthoDB" id="282595at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035822411" description="Beta/gamma crystallin 'Greek key' domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="2">
        <location>
            <begin position="545"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8165144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="633" mass="70776" checksum="7812A61B77FB6757" modified="2022-10-12" version="1">MKNMTLVLVLLVCTAVQVFGQQVVVSQSFTANDFTDADGWTVAGGLAHVTECSGTKMFGGFGKFGARAVASKVIELPPHSLINLKLQFWKIDSWDNEEAYVFVDDQLVWSRRFQFNEGEGQKCGQGGDWREMIVNLNLNVKHTGPTAVIVITSNLNEAADNESWAIRDFILSVEKCPEGCAACQADDKQENCKFWQSFASSWTALDSNQLGADGWDVAGGQAHSTQCGPAGVFGGLDKMMARAVVSKVLRVKPHYKLKIKVLWAKVDSWDNEAAQIKIDGKLVWERRFQWYEGYFGKICGCPVFEWKSMFARTEVDVDHTGEQVKVEFTSTLDEIVHESFGLRDLYIFYAACADNCAECTGPKDSDCKKCANNWALVGGKCQALPNFILLEQSFLEDKFTGINGWILTNNKAGRTVAECNGKSMVGGFDIMGIGASAKKTFEIPPHKRLRLQSTIYKIDSWDGEFMIIKVDGTDVWKTSWNLQTGGANICGQGIWWDGFTNVDEIFNHQAPKAEIVFTSTLDQDAIDESWGFRDFKLWYEPKEACAIFYSECDFKGASFEFCSKSPDFQKDNIPPQIRSIKIPPQGRVTLYENTDYNGKKVTYTSDQACIQSFDFSLIQMSGHVEGGWIEVEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UMG4</accession>
    <name>A0A8S1UMG4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HIT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0410310</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Required for box C/D snoRNAs accumulation involved in snoRNA processing, snoRNA transport to the nucleolus and ribosome biogenesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BCD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UMG4"/>
    <dbReference type="OrthoDB" id="272357at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070761">
        <property type="term" value="C:pre-snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000492">
        <property type="term" value="P:box C/D snoRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048254">
        <property type="term" value="P:snoRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23023">
        <property type="entry name" value="zf-HIT_BCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051639">
        <property type="entry name" value="BCD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057721">
        <property type="entry name" value="BCD1_alpha/beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007529">
        <property type="entry name" value="Znf_HIT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13483">
        <property type="entry name" value="BOX C_D SNORNA PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13483:SF11">
        <property type="entry name" value="ZINC FINGER HIT DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25790">
        <property type="entry name" value="BCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04438">
        <property type="entry name" value="zf-HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51083">
        <property type="entry name" value="ZF_HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HIT-type" evidence="7">
        <location>
            <begin position="11"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8164929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="263" mass="31135" checksum="BF66861BDDFD8A4E" modified="2022-10-12" version="1">MIEDGEISELCKMCGKPDKYKCPQCETKTCSLDCCKKHKQIYNCNGIRDPTKQPNKITELLVQRDYNYVNEVMKTTEIIQKKLVGITALPDAMRYKLLKHYARKMNVDVILAPKIMKKHRQNLSFYSIKDKQIHWCLELQYLNVSYVTKPISQKTILQDAILEQDLFDSKLLDQYKINTSKDSLKSFFEEGKIQLCWQSYEVLDFTEQDEYKFKNQKKRLNQVNSHQKQTLFIDPKITIEQVIKGYTVFEFPSFEIKFLLQPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UMJ2</accession>
    <name>A0A8S1UMJ2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0440040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8166240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="340" mass="39681" checksum="DB45CEE7B06F143E" modified="2022-10-12" version="1">MINKDNQVMKKKYYLNDSNLFSQNITIFKGKFSNDKNLENEIFGQQLMEMMKNNQCKLLDKISNLNVLVKVDHMMKMEIPLKLNGKKIGQWNNLYKEDKIQQILYKLHYYNLVVGVDPTIYYVMKQRLVWVEVNEQFKDNFQITQKGEYKNGKKSGQWNILIKEKQIGVAVMMKIKIKYKLESGLKQLSNLKIIHKLLILVNTKMVKKLVDANVYYFHSFQIQINGGGLYKEGGKCIKVGNWIDISEAFKDNSQVTFSGEYKNGLKYGRWDIWQKMIEEIIKIQKCTKYHIKRLILTCVSGGGSQDEDGNGFKXAQYQHALVVVNIKMVQNMVDGIFGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UPS0</accession>
    <name>A0A8S1UPS0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0460090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8166831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UPS0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="310"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8166831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="349" mass="41511" checksum="44779D00A12CF52B" modified="2022-10-12" version="1">MLDILLAKNTFVFQQAFNNTIKCVRTQVQPIIYPQLQQPKQHLYQPLQLNQSQTQQGYKKQRINIKKFLNFMQQQTIFTPQIKKQQDSVNHVELSLAGYMEKRSFNLQILKYFFLFQTFPLILCYCSFKNYFIKFSYNLNGYFKQPFYIFATIMCFSLIYSYMNHKKLRNKQLRIIQIILIILYTFSYAFFIQAIYSSTYLFYNPYNCIYENTLKNYGNDQIVIILLCLINLETCSIFSYCMQEIGELSIKNSLLPTVTFGSILSSFYYNYDILCFMIALSCVLGFYLQFTLKRMINSKKFKLLSDEGRFSAMLLSFILFVPFFDLVYEDSEQLCQITQSVHSEQKNVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UT46</accession>
    <name>A0A8S1UT46_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0470234</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000047">
        <property type="protein sequence ID" value="CAD8167664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UT46"/>
    <dbReference type="OrthoDB" id="296452at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8167664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="131" mass="15572" checksum="ED937FB96557B12F" modified="2022-10-12" version="1">MGCAPSAIQYEKVEFLKDDLILSDDLELSDETCANLINDSIRVNESILNRKTLYKEEIEDFQIIQEQQLNQKPFIIKSCFKRNNKTQQIMQKEKKVRFCKSYQININGLKFIVKKKKQGRNKKEYTIKQFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UUS9</accession>
    <name>A0A8S1UUS9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0470228</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000047">
        <property type="protein sequence ID" value="CAD8167652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UUS9"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="33"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8167652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="502" mass="57734" checksum="FADFA27CFA116FD2" modified="2022-10-12" version="1">MANRLKTQIQFSDDQSNLNSLEPIETKRFEDHYECLQTIGQGAHAIVKTARKKGTEEIYAVKIVRSGDQEIQNNVKRTFNNTRCLRHPNIAQDMELYINEKSETSYLVMEYCNLPSLESVIKKRPLKIEELKVIIKQLLFAIQHSHSKGICHRDLKPDNILVNIKENSNPPHVKLVDFGVSRRFISKGQEIDMLTKTGNIFYCAPEIYHKASYSKEVDIWSIGIICYQCTFQKLPLHSNELSDFVDLLGNPEKWTFKDSLCNIEMPLSNLILSMLNPNKENRITVEEAIRHPFFEIGTIRDVMALLNKDKIELDNCGRCRSLQNSLKLNEQWGDVIKKLQSSSNEDENINMNDLVRDFGNIHIIQRHTDKCGLIHLMNSVGSSNAFMSKLGSRQEILDKSCGLQFCGSSNKTIHQDPFGDVCNIQSSLEMKEEQQNSNNRLFQQLGTNLDYSLEVTPSEWQLTNQKQNIQQQQGTKKRGSQVNRMFNELGIKEVDEITEDQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UV77</accession>
    <name>A0A8S1UV77_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trichocyst matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0440107</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UV77"/>
    <dbReference type="OrthoDB" id="305857at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035933833" description="Trichocyst matrix protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="73"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8166356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="342" mass="38634" checksum="1AFB3AF1E1F6395C" modified="2022-10-12" version="1">MRIAILLVLIAISSTTETHLTAIKQLREKTRITPFSDYFYGMLELHQSSKLGEVVTIVQSMLDKLIAASGANEAEHSSQQANYEQQISKLENTLETTESELSSVNQQINDLTSDLSSLTQSLSFQGQQLLLLSNQITQLSDNHQEEVRASEEKFNNNSRYLAAVQEVLRFLSDTLLGNTSLIERERAIEAAKQSLGDKHPISIMIQMTSQYDDNTIRRVITKLEGIAQNVQERLDAINTSSNNSEQQYETLRSQFETTYSNLDKDKQFNQRQATAKDNDLASAKRRQQELQELISTSKELLDQTRIAQDNENTSYTSKRSHISEEISIVQSALDLLSQLPAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UXU9</accession>
    <name>A0A8S1UXU9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0500149</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8169077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UXU9"/>
    <dbReference type="OrthoDB" id="303045at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8169077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="395" mass="46618" checksum="6A1CD28546DD5701" modified="2022-10-12" version="1">MIHKNVVFGNHITANKLERLVKKQKILLKSSYDTKETKNRKICKSKSFKFEIHPRTFQETHNSPLKNQIQLELPSERSPIRLASSKPILEFFSRSQKSMTSLQLKNTIIANKLQRRWSNYELISEKQKRLNNKAPKSVIKYQYLMAVKAKKKKIQRMKMQYKFLGKKNNKVKKTRSKTQPLDPVIKMRHKRAQTEIKEYNEGLIFPLEHSLIRKIVKLIMMKKTAVKRQTCFQIPKGSMLLSKEKLIRFQQQINEKLVSQSESESQIDGSPCLDSLRAYQRRVNFEEKKFQIKIMSRKKKNLIKALQNKQTFSIINSYVSNKAKTQNSIQQQSFQNSLHQKSTQQTLCDFMSKQGDRLTTFHSTHEQPQIQQKTKRSNMLTKLFPYLQPQKNISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UZA5</accession>
    <name>A0A8S1UZA5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0530079</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UZA5"/>
    <dbReference type="OrthoDB" id="289809at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8170478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="121" mass="14918" checksum="728CC27324BAD2EC" modified="2022-10-12" version="1">MNQRKYEKQSRKSSQNKNQDQEQNPKRRWSKQDTPRNYDNINKLQYSFDYSTINNKYLQQIRNNTKIKQLIAKQKLEQQNSHLFKIQQDFMPKPKWQNYQDSLQSIINQQKYFAYLDYQAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UZH0</accession>
    <name>A0A8S1UZH0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0480073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000048">
        <property type="protein sequence ID" value="CAD8167886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UZH0"/>
    <dbReference type="OrthoDB" id="270720at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 5-kinase 8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF179">
        <property type="entry name" value="OS10G0565000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8167886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="308" mass="36154" checksum="93F0BFD9FFEF5904" modified="2022-10-12" version="1">MGSQCSQCNCDKRTFEIQECVSIQTTSSKTQKTWMIKNKMATVIQKYWRGYIVRRKVDDQKILYAFETETVESGITINHDDQTRIEKPTYTFPGGATYTGEWRGNSRDGYGIQIWSDGAKYEGEWKHNKAHGRGKFTHINGDIYEGEWENDMSNGYGVYKHIDGPKYEGEWFNDKQHGYGIELWPDGSQYEGYYQNSLKHGKGKYQWSNGQKYEGDWVENKISGRGILIWTDGRKYEGDFYLGNMNGRGIYIWPDGRRYEGQYLNDKKHGYGIYDWGDGRRYEGEWENGKQHERKIWTMVQRQTDSMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UZI9</accession>
    <name>A0A8S1UZI9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0520122</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000052">
        <property type="protein sequence ID" value="CAD8170065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UZI9"/>
    <dbReference type="OrthoDB" id="297512at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="199"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8170065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="331" mass="39499" checksum="9D3202D58CC84267" modified="2022-10-12" version="1">MNKKHLVDEMIIIREEMRNINNRIQTTNSSKIFECVPIQNRNEKIRKYCYQLVALRPKLFRKYFVSEQYIHELLNRMDYEKLLQISLKFIVKYLQNQEDEQSQINESTSPTNIKDVDRVTKIIKDIVSPIRTKIISQAQFFDHQLKCIHKSCQTQSSNQDTQSKNNFNLGIRQQSSILSSIQQDNLNSPKFNENIINVIDEISFERSSLKKDNQNMKQQFEQQENLQNQKLNQSQIKDQKVNYDISVITTPKQKELDNYKQTQNTSMNSSFKDTKRKLNNKSMLQLKHLEKLEDKRFPKKEVPYTMLSVDYVRKRRKTPEKNRGFNIITNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V0L2</accession>
    <name>A0A8S1V0L2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0520116</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000052">
        <property type="protein sequence ID" value="CAD8170053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V0L2"/>
    <dbReference type="OrthoDB" id="303197at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035800472" description="Transmembrane protein" evidence="3">
        <location>
            <begin position="25"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8170053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="298" mass="34665" checksum="CB29D507A2AC7704" modified="2022-10-12" version="1">MNLSAVRSIVSFLSLNALLQIYENQPVEFWDSNQMMSHPKLTPIFQSNQYINLLVGLASLALLDKNNKLHMSNSVLVGTSIIIQNLNLFMPKTMNMITIYFQLIILIVQLFQLLSFYYDKIPPTMPKIKQDIMKEQKQTRKPNQRKIVSNKKKEKLQQKQQESEQIQQIEGIEQEQQDKSEVESIENDFNEFMKPQSTDQTVTEIETENLYEINHADLSQISQTKEDSKLTFQLKFNGEVSTLNCTFDTFDSAMSILNSTHTSEQIKQIKIHLLLNLYDENEDCTCRLWCLKKLLALN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V437</accession>
    <name>A0A8S1V437_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0530141</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V437"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="190"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8170602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="232" mass="27260" checksum="1022E898AECFEFC5" modified="2022-10-12" version="1">MNFNPIKVQLILGGDGQINYLFGTTDTDIISIKFKPIKCAVCDIQQEHNSIIKYYVNWGSSVEYSHIIGLCQYHQYNNINKLQVEHFDQKDVGNYYLNQTEEIIVNITVDKQSLHNQLYISVRASILQFNNIPIGDYELYYHIAEVGLPMNAFYMYKNRFYEFLIALSILVVFTFSMLCCLCSFYRKALKLKQENLNMQLEQRMESAKEKVQIEVQMGYDQFQDENNEKSNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VAJ7</accession>
    <name>A0A8S1VAJ7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0590173</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000059">
        <property type="protein sequence ID" value="CAD8173533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VAJ7"/>
    <dbReference type="OrthoDB" id="307777at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8173533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="247" mass="29263" checksum="3AC3FDAC066607C5" modified="2022-10-12" version="1">MNNLYQFDRSLLTIDIPGAQPRKKSIQLNKIESLDIKQTRQLKLQQQLNIAQQNQQYGRRRYQHDFHHFCNSFVPQGENYFDGSQKKHYNLQEGEIKQMRALENIYDQPKLLTTNDIDGAMPGSLQSKVVKNMEKAQKMRLERESQRALNNEVLYLRPIMDSRLQNEKQPARTKSFSMQQTPQNPPIENWQNEINNLKQLNEFQKLPRIGENLPSIQNRVHKQHSFIEGFDREQSILQKCQPMKVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VB43</accession>
    <name>A0A8S1VB43_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0600034</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000060">
        <property type="protein sequence ID" value="CAD8173743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VB43"/>
    <dbReference type="OrthoDB" id="295031at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8173743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="451" mass="53537" checksum="17FCC36336ABB929" modified="2022-10-12" version="1">MKSRFLPTGDKVIVPDIRNTAEYGKLFEEEIFQSKIRAQREYEMKLQEFLDQVKVVDDFIFNILDIISSSLQNSKTPDPSLLSELKKLRLSLKFDDHYKESIEPLNEALLWIKASQHLFSVINQINLLRNIICAPLSQDYIRRMKNFSEDFELLLKEHKSIEVKSPFSFKQQKQPYLESAQQFDELLLRTLKAMKHIQQYSTYSMQIAAYYTEFQYHINGSIKVNQEYMELIHSLVLFYQNESSQFTPLQKALEGIPNNGIFYEQLENVKNLLHNYKKQLNLEDFKELDQIFQKYLKQQQPIFSLEQLRIDLFFILNDEEDFTTVMKSFKRVCSQSILDLDSYDDLLKELINFTSIKRDNKIAQLEDNFIIVSFVQNDKKDWQQLLKLCITKLGVFLKDAEKNQVENPQHYLNDFKHSASQLRPQKNTYSNSNLLQLYDKIIELFEINLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VBU7</accession>
    <name>A0A8S1VBU7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Kelch motif family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0570041</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VBU7"/>
    <dbReference type="OrthoDB" id="290664at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46344:SF27">
        <property type="entry name" value="KELCH REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46344">
        <property type="entry name" value="OS02G0202900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="306"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8172336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="819" mass="97331" checksum="F60BA17C6963C6B0" modified="2022-10-12" version="1">MYYYNQRIPVPLQRIEFKHILSGLQKHSQSQDEIKQKPKETRKNETCDYQALQTSDTIWDDRILQKLEKLKKQPNEFFQKIPKDPITLMNSSKINKFFEIKLLAQSKEDAQKVSRESQRQLNQIQEVAVKELLNKYPHQFNFTASADARKKKEVLDTKDKYMKIFFIRNCSTSMSKCKMNNKQLHNSEDSQKYIENQEQIQERKKNKSNLSFCTWSKLQELECPGYCNFIKQLAIKNKLQQSCSQQKIMEDKCKLHQKDFTYICIHPDCFKYKRLCNDCKVNHESRHKKNQIYQNKQFTITIQECKQQVEKNLNMVKEELELAENRAANALITSVNSNIDEIILEIESIRQQIHKLFDKYIQGLRDKFEYKVKQSIHALYDQIQGQLTQIRQFQNDINSIDTQKKVMSTDLDYQVQYQKNRLESLIYQRANQKFYGRIDDDVALHFIGVIQKELEQLVIYEKNKLSSQIESLNQMTLQAQQTKQKSIKNFQKFLSYAPEKLDEMKNTIQGTQEYSFYRSWLHYFEPGTKFLYYLDLKQQNPQITKVELEIDFLISQGSRSILASDGQIYYFSGYDNGQNDDENKTIYQYDHPQLSFIKKTRMYTLRKNFSLSCTVDKDIFIIGGQNYKEGCLAKCEKYNINTNQITLINHLTELSTLHSTCTYNNKLIVKFGGLIANTEGEDLRKQCQGLLELYFIEQDCWERVENTTNHTQYPLHTILSTSIQILNSNMIYIFGGQILQKQSFQTINQGFMIQIEEEEEFDQDQIKFQPKLYYQLQHPIPQQAYFLTQPIYYSNKIMCLLKDDQMIRSCYDKNKWKTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VG88</accession>
    <name>A0A8S1VG88_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0630063</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VG88"/>
    <dbReference type="OrthoDB" id="260091at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016717">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042759">
        <property type="term" value="P:long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006636">
        <property type="term" value="P:unsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03506">
        <property type="entry name" value="Delta6-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012171">
        <property type="entry name" value="Fatty_acid_desaturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19353:SF19">
        <property type="entry name" value="DELTA(5) FATTY ACID DESATURASE C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19353">
        <property type="entry name" value="FATTY ACID DESATURASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="2">
        <location>
            <begin position="12"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid desaturase" evidence="3">
        <location>
            <begin position="128"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8175453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="342" mass="40849" checksum="C03F5BE46E495586" modified="2022-10-12" version="1">MAPLPEPNNQNVQSEQNNEVFRFIYKNKEYDVTEYVPKHPAGKSFFDKMKDEKEDFTEYFRCLHSKKALKILKSQKVVRSNIKESEESKQYTHIKKQVKNLFEPDWTIELLLFLGLSLGLYLGVTSEWYIAIPTIALTQIVAGWQGHSTNHNRNPLLYKLSIPYGIIHGFSADWWQFKHNNHHIFTNRIGKDDDINHTYQKWQYGFLYLKWKFDSFLTSYNKIDIIYILIHQLIVFQQKIWIYVVAQYIAGFFSACILIGNHEREYKFFNKIDKPFIEHQIITSRNYDWTDWLSNLLMGGMQFQTEHHLFPQIPFYRLPYAAKIINRELNKFGYKIHVGKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VGY6</accession>
    <name>A0A8S1VGY6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">14-3-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0620107</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8175009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VGY6"/>
    <dbReference type="OrthoDB" id="10260625at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08774">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000308">
        <property type="entry name" value="14-3-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023410">
        <property type="entry name" value="14-3-3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18860">
        <property type="entry name" value="14-3-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00244">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000868">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00305">
        <property type="entry name" value="1433ZETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00101">
        <property type="entry name" value="14_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="14-3-3" evidence="2">
        <location>
            <begin position="4"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="201"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8175009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="240" mass="28985" checksum="D2346CC58FE2F1E4" modified="2022-10-12" version="1">MLSRQELVYMAKICEQAERYDDMLNYIRQVAFMEQELSTEERNLFTFAYYYCVGSRKTQMRQLCHLENKVTQKVNTNPQFSAIIVLKKRILNELNDFCNEFLILIENQLLKLHQQTELYFQYQFFKCRYLKHKVDYLQDEQERDIAINIALLQFEQIYEQVIAQLLPTNVRRLQMVLDFSVFLHEILEDNKKACEICDEALIQARKEIDKELNQQEKVQIEQVLNLIEQTRNLVMEDMKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VQB4</accession>
    <name>A0A8S1VQB4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0710140</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000071">
        <property type="protein sequence ID" value="CAD8179227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VQB4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8179227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="44" mass="5235" checksum="C0DB43FD11506FD8" modified="2022-10-12" version="1">MTIEIKTQQDYSDMDVNIEEEHTQAKVQVKVIRNKVVQLEDFLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VRD6</accession>
    <name>A0A8S1VRD6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">uracil phosphoribosyltransferase</fullName>
            <ecNumber evidence="4">2.4.2.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0690061</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uracil: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPRTase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.9" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VRD6"/>
    <dbReference type="OrthoDB" id="106623at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004845">
        <property type="term" value="F:uracil phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008655">
        <property type="term" value="P:pyrimidine-containing compound salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000023">
        <property type="entry name" value="Probable uracil phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050054">
        <property type="entry name" value="UPRTase/APRTase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001097">
        <property type="entry name" value="PRK00129.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32315">
        <property type="entry name" value="ADENINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32315:SF4">
        <property type="entry name" value="URACIL PHOSPHORIBOSYLTRANSFERASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14681">
        <property type="entry name" value="UPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="10">
        <location>
            <begin position="58"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF14681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8178242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="272" mass="30740" checksum="04D49CDF83BE3770" modified="2022-10-12" version="1">MFRNLIKIGGCLGLLSSSNVLCNQIKGKNQDDQLPHYHFLNDHDLQKLQKKHSNLSIIDKSYTEHILSVIRDVQTDIVDFRKNADRLIRILIEQAISQIDKKKHIKQSPLGYYDAHELKFQDEEICFVSILRSGNAFLIEALKVMTGASIGQILIQRNEETSQPAYFFQKLPQNIKDQQVILVDPMLATGGSASMALQILKNHGVKEENITFLTLVSCEQGLGKLFSEYPKIKIITAQVDPILLKNINYLAPGIGDFGDRYFGTVKTNQAQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VRH4</accession>
    <name>A0A8S1VRH4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0690104</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VRH4"/>
    <dbReference type="OrthoDB" id="298386at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="63"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8178299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="339" mass="39610" checksum="2977F45EAFCBAD1F" modified="2022-10-12" version="1">MSKCVILFDDDKTQCKTLLHSKKMMTCLNPDCLTYPQICCSDCIEQLHQHKGNSMTLEKSQFVNQTNKKYSEIQEQVYALEAEYQNFEKKGSNLKRLFNGDIERMRIQLNKIIDSLKEQINKQIDLSISTTKKQVTQKIEKTKDALQQFKPINISKIYNDQLKRLINAMWHTDYKLERDQLKIFFPNLQEKLRGKLSQINDSVSQELVQLSQELIQESQEEIQSFIDQRTPNDNLSTQSDESIVNKLVNLRDSLKLQMEQHLAAKNCTHKGCHQVTPVKAYYGYYYHISGYALQGELPISVCAKSKKLAEKMDRVVNDLYKLQYRDSTIDDCVQAYFIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VT21</accession>
    <name>A0A8S1VT21_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0750050</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000075">
        <property type="protein sequence ID" value="CAD8180824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VT21"/>
    <dbReference type="OrthoDB" id="285201at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032752">
        <property type="entry name" value="DC-UbP/UBTD2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039869">
        <property type="entry name" value="UBTD1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13609">
        <property type="entry name" value="UBIQUITIN DOMAIN CONTAINING 1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16455">
        <property type="entry name" value="UBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="487"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="166"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8180824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="534" mass="62374" checksum="B5901DDD6D9709D8" modified="2022-10-12" version="1">MGICTNKQYAQTSDPPQNQRQNRLYLNYQQLANINHPLNVNHKYNNYFQIIPDTTVGKGIKMTNQYTSRLSREEWLEKRKEFWESRVEGEKVYWQSIQKAIEEQDEANVQAILNACDLKLVNNSIQLLYDNSMHKYDVPVFMINEPQSFPSTNFCDAGLIQDFQETELKVKVRSNKLPQDFEIITHTNQSIAEFKTKVQESSPESNQCRLFFKGREMKNHHLIGNYQLKNGEVQGYKITSEKYIVFQRKDEIINNRPRYFNTKSQSLSSPQEANLYTEMKISDILNKKPDQITEEESFNVKINSSFLNNSLSNFLDKQNIILNPGDVTNQPVNNIMSNTVNQIKLFILFMIKIKVQNKLLIKILGNLGQSDKPIVDQTTFSRTFFTDLARQTQSVTDSWSKIPKTSNIELHKQLLREEMNIYKKKQFALNYCPNFEFGRKQLGSCQAPLDKEKFNVYSKNPQRQLFRNPTVPNFIKLLERELDGISLIPSFINIPILVWELIIWIIKCQNSIILEMVTVTTSFMLSKIKKIQPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VUS7</accession>
    <name>A0A8S1VUS7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Piwi domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0730099</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000073">
        <property type="protein sequence ID" value="CAD8179993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="445936at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Piwi" evidence="2">
        <location>
            <begin position="232"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8179993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="354" mass="41431" checksum="183A766204C67298" modified="2022-10-12" version="1">MKQYQKSFIGKPIDRNLIVRSTLKLPQISIGTMNIPNRSSEHIPNSRLHMKTKTRDTIKAVVKERPHFKQLPTDRTMPTLPSLNSPRSEDEGESSHKNLFSIRRTVIDTLSLRQSRRTSVEKDRLKEEKKEEDRPDIQQHNTDYKISQDQQIINQQNLKLSKSHDQFIQNHDNLDEVLEEPIEEIRPKKESQSIRPKNESVSSQFTQLTDEIHSVWDNVDLYLRGIQFDXINDRFFMVNSGNHQEQKQQLSNPPSGSVIADKITSSYFDYFITAQYVTQGTCTPTHYRVLENNTNFSEELFWQLTYYQCFNYQNWTGAVRVPSCVQYAHKLAYLIGDTYQGNIHKRLAHLQCCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VV67</accession>
    <name>A0A8S1VV67_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0690053</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VV67"/>
    <dbReference type="OrthoDB" id="291304at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8178226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="198" mass="23656" checksum="8ECBC8D8B3F1E22F" modified="2022-10-12" version="1">MNLENPNKLKWKQLYIKKYRDLLGISNLKVSEAQPMDNHITPSYLDHIDRVSKQRSQSMKCNDESFQSRNSSYSVNRKLSEMNSTKIYSAAEMNQIIKHAKLCGLLHNNKPQHQPLLEQNRKYFEEVRNLQKSWHYSKIRQQEQNIQFNRIPMEQHYQFKLNHKRRDLNIQRLIQEQKTIYLSHNTQAIEATVSSGQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W0L7</accession>
    <name>A0A8S1W0L7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0820017</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000082">
        <property type="protein sequence ID" value="CAD8183858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W0L7"/>
    <dbReference type="OrthoDB" id="5855668at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04048">
        <property type="entry name" value="C2A_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04047">
        <property type="entry name" value="C2B_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000351">
        <property type="entry name" value="Copine-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000409">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037768">
        <property type="entry name" value="C2B_Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045052">
        <property type="entry name" value="Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010734">
        <property type="entry name" value="Copine_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857:SF106">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857">
        <property type="entry name" value="COPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07002">
        <property type="entry name" value="Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="125"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="551"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="669"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="551"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="573"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="589"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="669"/>
            <end position="701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8183858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="714" mass="82332" checksum="F261F58314A99FFF" modified="2022-10-12" version="1">MNDLYGRSASIMESQQKLELFISCRGLANMDTFSKSDPYVIMYVKRNNQWSEVGRTEIIQDNLNPNFAKTFIIEYYFECQQPLKFICNDDDGNGQFDFIGSVETTLANIAGARDQLLMLNLSNGNKKTGVLIIRADQVRMINDKIIMQISADNLPNTRFLPWHCTSPFYRLYRTRKDTNQQLLVYESEPMKSTLKPIWKRLDIQSQKLCNGDYYMPIKVEIWDYRTSGSHEHICSTEFSVDELKGKNMVKKILFDKNKKQSGSICFNEFKLIEKPSFLDYLQSGTQINLISAIDFTASNQSPKNPSSLHYIDDQHHRMNQYQQALLAVGEILLNYDHDKKVPIFGFGCKPRLHNLNTPQTLHCFPLNGNPQDPEVFQMDGIMQAYNYAVRNVQFDGPTYFNPIIQESMRIAQTCKDMNTNTYFVLFILTDGEIHDMKQTIDSIVASSHLPLSIIIVGVGDADFTNMSILDDDDGNLHDSFGRRTQRDLVQFVPFNQFKSNPELLAKNVLQELPDQLVDYMLLIGKKPGPKNFINLGQFDINQYSQQYGLQQQVMQPNQQQQSVPQIPQQSFPNLPPPSQFQQPPPPPYTQQVYQPQQPSYQSQQLPQYPTQQQSSYPQQQQQVYPPPNYQQQQQQIYPPQQAGFQSKFIQGLANTNFLGQHINDAQQQQLNQQLPQFQYPPQEPENPLGMQPQPNQYQQVKQPPPYVPDNPYGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WAC8</accession>
    <name>A0A8S1WAC8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ABC transporter family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0860001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000086">
        <property type="protein sequence ID" value="CAD8185803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WAC8"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000151">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002273">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF456">
        <property type="entry name" value="MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN LETHAL(2)03659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="279"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="307"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="173"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="488"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8185803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="800" mass="92826" checksum="E9FCAB38E5C98EFD" modified="2022-10-12" version="1">MKEQNIKYMNFNYINETGQQMSRLKRNQVSNFENPEMSTLKNIRLQTYPDENQESEHLHNSNNPSEQSHESIDLPEEDSHKFFPSFIGQNHSRISKLFFLQYTSYALRLKNQVLDKNHKITDKHLPILSPDDDLKQLISKSQNQILEVQEITFITLMKLIFFGELKWITGKCIFAYIIESISKNGISFIMNLVIASVQQNDLKNSHFYGIILITLNFICLQSRHHAANYSMIFSTKACLNLINLVYIKLIGLNSYSFKQANIGKILNLISGDINTLEQMFSIIFPSIVVIFSLFFACLILWVRFDGIIGLLAVLLLFITYPIQVLISQFNQETLKLAKISQDKRLRITNELVEGIRLIKIQAWDKAFQKIIMTIRQKEFLYLLKILLRTVVDRLLTQTSHLWSSLLYFIILYYGEFQNEIQVAEMISILKLSCVYVVSNGIQAYIQIKVTFERITNLLNLQNFVMLKLEDNKKENNKKIEKETEKSTIELNNFCAYRQHSVSENDEPILKNLTINFKEGELWAIIGRVGSGKSTLLNSLLGQIPSYNGIMLINGQDPIMNKLKIAYVEQEPYLFPDTIRKNILFGKVYNHALYKKVIQVAQLESDFELMKFKDYTEIGERGVTLSGGKKPEYPDLYLFDDPLSAVDASVAEKIFTVAIQEFIFNYQIALYPKKQKPIVILATHQIQYAIKCEKIAVISNGELISQGSYDQIKPTLNMINSELADQLDKIKIENKTKDYQQQKPLIKKRKRVQLSEIKNLIIQESDIIKQIIKLNILISSFNYFIHHFKPIQPWKNTNFIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WAZ4</accession>
    <name>A0A8S1WAZ4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trichocyst matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0860130</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000086">
        <property type="protein sequence ID" value="CAD8186043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WAZ4"/>
    <dbReference type="OrthoDB" id="294361at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035809901" description="Trichocyst matrix protein" evidence="2">
        <location>
            <begin position="16"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="76"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="234"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8186043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="382" mass="43250" checksum="7411154214D305A6" modified="2022-10-12" version="1">MKNIIILVLVATTFATNLSAFEKIEKSRLGKTLLNTISLHMQTGEPLERVFQTLYDLEERYQNDQREDDAENKVFQSQVDGDLAVLNQELANLEQKGTELQARLDELQPQYDTKVGQRVAKLAELTLVQKVLNEATEKRTSQNNDFEAQREEFTFVSSVLAEARRLFTDNLQAPTFLQKGSSESVHATPAIFAQVASHLNSAAHKASQMKHIKTYGKAIKLLAQLSIKAQQFANQELTSRIVQLIDDLQNQLQQAFDLARKQEDAKRQQFEAYSTLLQRDINKLNSSIANLDAEIQSLADQLAATHSSQDDNNDRYAAKLQQRDDRRAEGQQAAYEYQQRRSARDADRQTVSDLIGHLNTNLRDLKEYIAQRVAAGDNDLGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WEC6</accession>
    <name>A0A8S1WEC6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0890144</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000089">
        <property type="protein sequence ID" value="CAD8187573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WEC6"/>
    <dbReference type="OrthoDB" id="10248513at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000039">
        <property type="entry name" value="Unplaced genomic scaffold supercont1.13, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10209:SF874">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10209">
        <property type="entry name" value="OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8187573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="357" mass="40688" checksum="4B573E55D4C587DF" modified="2022-10-12" version="1">MFTTRFVYRFAKSVPVVDVTAFLAKKGDTSAQCAQVAEALHNYGAICIKDPRVNDRHNVDFLDMMEKYFESRAAKFYNNEKVEDIFPNYSYQVGATPEFVEKARTHNNIVRQYTKENEALTPQPAPYDAKWRFFWPIDDSGKRTKDEDFTPPRFRPKDVPQFGERMEQWGKMMINGCLTVAEMAAIGMSIPADSFTSRMQNGPHLLAPTGSDLDRHKPGTIFAGFHYDLNFLTIHGKSRYPGLYVWLRNGERISVAVPEGHLLLQAGAQFDLLTGGYVTCGYHEVIYTEEAKKKYEENKKAGKSTWRVSSTLFSHINSDVTLQPLDKFAKAGLPPKYKPIKAFDQVQQELEAIKLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WEW7</accession>
    <name>A0A8S1WEW7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0890113</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000089">
        <property type="protein sequence ID" value="CAD8187511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WEW7"/>
    <dbReference type="OrthoDB" id="1734229at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031207">
        <property type="term" value="C:Sec62/Sec63 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006620">
        <property type="term" value="P:post-translational protein targeting to endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24075">
        <property type="entry name" value="SEC63 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24075:SF0">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC63 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="195"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="103"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8187511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="464" mass="54068" checksum="C94A846722BCE969" modified="2022-10-12" version="1">MSDQEFDPSDFAFLYFAISIIIVAMLPLTYSLIKQPFQSIKLNPKYKKAPATAKKAIEIDIQRNLFYKKKGFYWKLFFWILLVMIFINTYQQLPDPQLMKGFDPYEVLGVKSYTPIDQIKKAYRQLAREYHPDKHPDETQKYSKLFDTITKAYQCLTDPRKIANCKKYGNPDGFTGFQIGIALPEFAVSKDNQGFLLAGLFFIFIMIILITFCTAFSGMGKFDVNGILMSNRKHLAERIAAGKNKSFEECLETISQCEEIDNKDFINTIQCEHAPGILYMSIEICLQILTQLWAHYELKQIRDITDQIIVQIESIPKIIQYNSNLNHPGKPKLDANLKTSEETLTEDDYRNTDLFEIKCNLSGLIDQFIQSQTFPVLKQNQWNILLFYNNMIIGSRQVKEDQESKSVVFRIIPAQFEQKNITEFEFRVVAYADSYQLKLEQKINLKCSLYQEEFKKKSNKQKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WF98</accession>
    <name>A0A8S1WF98_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0830157</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000083">
        <property type="protein sequence ID" value="CAD8184626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WF98"/>
    <dbReference type="OrthoDB" id="309418at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38019">
        <property type="entry name" value="KDA ANTIGEN P200, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38019:SF1">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="268"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="370"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="472"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="377"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="390"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8184626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="504" mass="62845" checksum="9F5F360CFA6C778F" modified="2022-10-12" version="1">MITLINFNPADRNCFIDSPRSIQVIRQRGIAQNELFCINKEQLQAMAKKEKSLTNENLDDFHKHFEYRRQQKVYTLVKDREGLIEKEKRNKIEQSTYSLKLLRPASSTTTSKFMDQVQKRKQMEEQRQRHLQEMMLKREEQTQIREEKMQLYEEKRKRIQEEKQLEFQEKQENITKQKRQKELEEQKKNKQLQQVYFEREQERKMQLEFEEQQRRDEAAIREQQQRELKQQLEREKKMKDKQKEQEYLIRKQEEEEQELKRQIFFKQQQEQKKGVAQKRALSAKERQREAQKSQIEGLKKLREDYYKKEEKIVEKQYKYQELQKERQLTIEEKNRIKNQKIKYTAKLKEHEQEEVRREFMEKEMDIQLRLQQRSQSKSKEQYMQEEADRRTKHREKVKKMNMLQLEEKRNIYLRKIEEQDEKIEKVKNEQEITLQQQAYMNLKKKVDQQEAIKHQKQLEEYKREKIREKLKQVDMRMDQKKEEQRELDRLREQANRIEMMGRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WFB2</accession>
    <name>A0A8S1WFB2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein 21A/21B C-terminal ARM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0900152</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8188003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WFB2"/>
    <dbReference type="OrthoDB" id="10262375at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056834">
        <property type="entry name" value="ARM_TT21_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052628">
        <property type="entry name" value="CFAP70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44314">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44314:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25063">
        <property type="entry name" value="ARM_TT21_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Tetratricopeptide repeat protein 21A/21B C-terminal ARM" evidence="6">
        <location>
            <begin position="634"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="704"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="62"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="280"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="62"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="87"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8188003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="888" mass="104544" checksum="C2D28B6A482B17A5" modified="2022-10-12" version="1">MEPDPKLLSLLEKVNNPIETLTIHLTIKGVYGFTDDWRITDETNPGIFANVIRYQGEDMFGKIRPREQTEKEKYEEYQKNSKKNKKESKSPEEEEAMKKAIAQEEEEEQQKQQHLETLSEQEKLFFISEDKFKTASIQYEETHSIVINNVRQLEEEILEKRNTIQFIRQPAVTEEELVKLKKGKTKNLNNAELTQIIFKGIFDLADLLEPGCTQTIVRVFLKQEEGSDCPKYNCEKLYMKIEVNTEPALTPLVEDVPSLKIEPVIIQKIPSKFECVWQFKKDIKEAMKSLEEEYSKLYQSDQKAKTQPMISMTHQRWLELTKKKESFLNEMNTSGKYNILQERLRSSIIRVCIDKFAKEGLFAGVNKDEQDRLFAELYVFMMEQMQTTLGEYIQNNKEDDIHEDLVLTFEQNIRERDRVFKRLKESQLEKFQRLAKEYETINQVNQALKYYTNMVLVDKTSASAFAKYCLKIQRFKAAEQLIQMVRDVEWNKENELLMACLYIRRERFKEATQILKELLQREPINTLFNLIMSFIYRQQGNDNMADKYLRCTQRIFMRTMGLLTKGFQKQQPDPHFLPNFKQQMIEQQEKAKKAPVLTQEQIDSIQLELIEYFASRNLFDLAEKALVAIKDKTTQKINIIKAQIHIFNENYTDALTLIQKLLTQNSRFYDVYLIKGSLCFQTEQFYEAEETFLKALSIQSKRSFEIQLRLGYLYLKRKSWQDAKVIFEKALDQQPNSSLSWLGLGIANLRLCDPIAEECLCQANIYEPFNGEVWGYLALNCLLQKRGQQAKQCLLRMEQTEVYDLDLLLELAEEMSLCFDYESCRSILMRVYDSKIIISNMGKLLMNLGQVHSQLGRNEEAKQYYIESLQYLESGNEKEKVNSEIKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WMR9</accession>
    <name>A0A8S1WMR9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0950085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000095">
        <property type="protein sequence ID" value="CAD8190007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WMR9"/>
    <dbReference type="OrthoDB" id="10268105at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049916">
        <property type="entry name" value="WDR72-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44099">
        <property type="entry name" value="RABCONNECTIN-3B, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44099:SF4">
        <property type="entry name" value="RABCONNECTIN-3B, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8190007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1274" mass="148887" checksum="A6951C803ED05844" modified="2022-10-12" version="1">MPNFIFPLLYRNKTLQNHVVEKIIGNDDILVTAGQNGELVIWKQDLNPRIVIYPNLSNAYGKLLTMNLVRLSFFNILDRAEQCLLTIHEDKRIRIFDCRDGRCVGISAHDSSGPVQYCVPLSSNNCRFLILGMQNGLSLFDLWKMKSVKQYDTKIRSLCQLDINTVCILDQENYLHLINLSPLNNERYLNFKEFKKKKEQIKLDFSELKLCDGLKKLKIDGIQSNRIRHMSFNQEYQHLTILMHRQLYLVFDIFDTPWIMCISQSKNNQKFTKVHYSKEYLYITTRNGSVLGLEYKDIIYLLSILKEQSDFVYGFKEFQFTKIADSQFSRLQINFQELISGSEDQKEHNGIFILKTIVYQYKNKCLCQYNLTDVKLDLSLIRINQSDSDIKFNGFKWSFNTFSDLIKIETLKQGCKQVDAFNFKESGQDDLLWNLGQMTTKEFQTKYLPYINIDTQITSAFISVNDKSQIYWFGCSNGIIYGFPTIYSSKSYFIYRIKMDEKTVTFIYQKGNYLIASSESGQFIIIDYSLVVNEKMGEQQVEFIDLEPSQRIYFPSYAKKTIRIHKLENESYEEMQSNNQQQKFQKIAILMDNNVILIINLQDGGVQLKLHGIYQQVLGLYYEDQGDQWFVVVQGGESLVFDYKGRYLRTLMIEQYHSLFQVEKKLSKVKDKMINHHQLSEFKREFNKSISKVYRLLEFQQRNNYIKMQSPYPSLYEVGGQFKQWQFEAEQAAKMLYLIDHNSVKSNMKMCKFVETNIGELEWWDLKKQGDNNNQNRLFLMLHPWEADTQLSTQLDQILNYQKPTFAPLIGVQSMGYCVSFALSRGWDVSPHFTSQRAMSLMHFYLSCLSKEAQVFNQLMIQTVELLTKKKQLLDSNFKSMDLFLLAQYTIDESNEVMNAACNLILSQIRKSDQDMQEQHKLIRELLDLNKEQQGHNFSNVEVILLMLESYLTGMCQNLQQSEDQKRRVIQGLNLKNCIVNPHLCAPLLKVFVDVQQHFRNSYNPSDIFKNLFFIFFQYNTVIEYSEILTQGSQQMQLINLLKEISKQSDDMKKRMRQLTAKSIIQVAIAAPVDFTKILNKDIVNLETHLFYPSSIIEIIRNYIKSQHQNIHKQLPILMDIILKSLDPNNPNLRKVCHRSATYALQTICKTFKCVCFHQQTQKIAIGDTQIIIYDLRTAVKWRVLDGHSGQINCLEFDHTGKQLASFSDTDWKIKIWKVGSTGFFGAIIGIQGKPAKDMSVRQQPLVHPQISWSQDDNRVFLMSNGQSVVQCQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WQ05</accession>
    <name>A0A8S1WQ05_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0960025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000096">
        <property type="protein sequence ID" value="CAD8190259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WQ05"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="75"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="120"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8190259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="339" mass="40090" checksum="93E706908171050D" modified="2022-10-12" version="1">MFFSNQENTQSNKQNVPLTNKSFNYQLIKNNSVKQDQICYAIAINKDVSIVLVACSKDIKVFEFQKEVLKQTQLLIDHQDYVNTLNFMNKSNRFISGSSDKQIIIWVQNQNNQWVFQEKLTGHTNQIRCLVLNNNDDQIISGSSDNTIKFWMKKQNEWFCSQTIAEHTQQLLALSLNDQQNKVISCGWDALILIIKQSQQDSKWMIIQKIKVEQYGCRICFIDDNIFTFQPFSEYYMHIYEMNKTNEQYSKVKHIDLKGGYDQWYFPQQYVKSKCILVNENKSCVYLIRKIGDCEFKIEQTIQFKDNSQIYGQMSDDGQYLIIWDEGSKEIQIRKYNEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WV41</accession>
    <name>A0A8S1WV41_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1010034</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome b5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000101">
        <property type="protein sequence ID" value="CAD8192225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WV41"/>
    <dbReference type="OrthoDB" id="260519at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004128">
        <property type="term" value="F:cytochrome-b5 reductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051872">
        <property type="entry name" value="Cytochrome_b5/Flavoprotein_Rdt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46237:SF1">
        <property type="entry name" value="CYTOCHROME B5 REDUCTASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46237">
        <property type="entry name" value="CYTOCHROME B5 REDUCTASE 4 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="6">
        <location>
            <begin position="201"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="156"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="156"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8192225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="280" mass="33001" checksum="57D226331E0FCFED" modified="2022-10-12" version="1">MNNKEEINLYIEERQDTLIWMQDEVELEILEEVVTFILNDIKQKFQTDILTLRDEENQYYEIDQIIEFKRPHTYITIPQRHDVKTLAMNQLRQNRSIYHISNEKVLVEKYKGTRKVESVDYSIAVIKKTNQKYLDTLKGIFDDLLIQPKKRNSVTQETQQTIQLQQNQPESPKFGQQPKENTSPSWISKRALNESIKKCELKQLTLEDVSKHNTSDSAWIVINSKVYDVTHYLNKHPGGKEQLMRGVGTDGTPLFMQHHPWVNAHYLLEHSQVGFLINHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WXP7</accession>
    <name>A0A8S1WXP7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0970030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000097">
        <property type="protein sequence ID" value="CAD8190616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WXP7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="7"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8190616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="117" mass="13953" checksum="2E3D4A59055762EC" modified="2022-10-12" version="1">MEHCDHCRETINEMFALEKKVESIKKQLEIAETQVKLLRQENELLKSNIKKPNKQKHQNQSIQSQIQKSYQEIAKLQDIIRHKDEIIQNQIRNDDSVSDKTEIHSKQIDTCRKKVKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X2G9</accession>
    <name>A0A8S1X2G9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1080165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000108">
        <property type="protein sequence ID" value="CAD8195181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X2G9"/>
    <dbReference type="OrthoDB" id="301262at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8195181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="429" mass="50477" checksum="D642A818E96AC6E5" modified="2022-10-12" version="1">MILSTRILQCFSSEKKILRFIKTCGSPKEIYQIYHKNQEHFTPLLFLEALKHMNTSPVRKRFDLNDDTAFYDELTEVFKQVPMQEKWRFYVQVAKIGRNKQLIKQYGSDLTCIRIKQIAILLWGYHKNGIKKPRLVIHLIDQLQQNLDSLIPVSEKPDEIQSLKENQKQENQEDNEINQSDEDDDFLEQELEEDLQSEKRVLRDFRFKFKDMVLIIWAIQDIKLDASALLIYTFKLAINNFNKKDLITNRSLILLLQATINIKDNKQINAYQKLVIQNLAEQDLSNEGILQLLLLLRTLGQLKQSPEFAQKLAYIMLKNQEKSNQPFSSKFACIMIWNLNKLGITDTDIYNKLGLHLTKSNEFKAMDISQAILIYSLQQTKSPQYQQYQFILRSLISKANMIKLDPRSQSIIKESLEQYQPHMIKLLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X4I2</accession>
    <name>A0A8S1X4I2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1150041</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000115">
        <property type="protein sequence ID" value="CAD8197308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X4I2"/>
    <dbReference type="OrthoDB" id="282937at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13913">
        <property type="entry name" value="zf-C2HC_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8197308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="236" mass="27384" checksum="AEF44339FB811208" modified="2022-10-12" version="1">MNKLAFPTDNENNEFFDEDAISKSTIQAVNNSFDKEQLDEQIERPFTPVDTQIEMIPKEQCPHCKRQFFQGRLTLHLRSCTTEHPFVKKSKNQKTQTLQIKPALLPCPHCHKKYRNLKTHTCQTRTKALDKYKVVAQINEEEEADETLIQELSSSQPSFLPKIKQRVENILSRSVPIVKCPSCQKTFEQKAYEKHINLCIRMNSGNKFQRSIFCTNCGNKFANNHKYCGSCGKKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X4R7</accession>
    <name>A0A8S1X4R7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1100009</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000110">
        <property type="protein sequence ID" value="CAD8195559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X4R7"/>
    <dbReference type="OrthoDB" id="299560at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="272"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="560"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="249"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="272"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="283"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8195559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="840" mass="99302" checksum="CB083C210E25EA3B" modified="2022-10-12" version="1">MKQFVNPVFQPVDYSMSISYHRTEFIPKIPQSTIGQEQAHDFEKIFSSETDISSRLNDSVIQPFDNRNFYIDQSPAYRGTIESSFNNNTLSITQVIEQKKYNQEIIKYSDDDSLINSSFFGILNQDALQKPLQQKDLIYKKFQEPYQFDHNTKNAQTICDEELRAELQYQVQDLHTINKFITPIQYQQFHKPIQDSSNNKQLTYENKNQTIDLYQSSDHNKFLQQPSDHNQFLKQPNLQDLKNKDQMKYTPSTYNNTYSKPIETNLIIQNTPPKSRFISPTLQKQQQQQQQQQQQQQQQQQQSQYYQSPQNKTENLRINSPDQFETIKITEIVKVDNFKNQSLTSPSAPVLPLRHSSFINNKPETQINKKNSEVYYSQMIQPIANQKVLQRTIVNRVQDNNQYELDSQLQQYSRKIQSPNKNNTFLDQQSQIQSNRTSQQQIIIPQPQKQQYTIQSPIKQTQSPTIISKQQERLDQKELFLQAEKAIKFQRTQPKAILPEWQNQQTKQEQRNNQIFSKNNGNKQDDLQVLCVICEDLIPFENVDSHSIRCLAKSKQQAKTQNAHILIQNLNQKLEQLKQNIIIQSKQVENDLVNTEEMINCIKKIIQCSYNYKKLKYYNELLEQQYQKYQQNFDQKRFIMILTLQRVLAVSQEKQLKLQFVEESQNAQLEDESYIQNEFIRLNKQVGIEKANQEQVKFEQELFKKIQKDDLENMKYLRAQYLEQNVDNEVLSQINSEWDVRNPDTKTNRVTNSIVSDIQYQRDFDGFAVRKEGSSPTMIQNEDQKKAFYQLAVQIKLNLPHNHPGKNLLLQDAYEKAVSLKVSRNVWEKFIIEEIRSIQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X4U4</accession>
    <name>A0A8S1X4U4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1100087</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000110">
        <property type="protein sequence ID" value="CAD8195705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X4U4"/>
    <dbReference type="OrthoDB" id="1668162at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051144">
        <property type="entry name" value="Formin_homology_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45733">
        <property type="entry name" value="FORMIN-J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45733:SF8">
        <property type="entry name" value="FORMIN-J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FH2" evidence="3">
        <location>
            <begin position="705"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="589"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="755"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1085"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="472"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1001"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="589"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="601"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="701"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="756"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1085"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1107"/>
            <end position="1117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8195705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1150" mass="131975" checksum="EF40DEEB9478F857" modified="2022-10-12" version="1">MGNNIPKDASFEYQIINKLGTISITYEDLKGNFTFPKMPNKKTVVLEEFRKFLDEIILLPSDKHIIEQLPLKIKWRLVHRHNIEKEKLGDLIPHLEESEKSEIQHLVDECVAKPSTIALQNLQRRLENPEEDEWNQFLVYRGLFKLLEVLEQCEINARSTKNYKLCVAFLRFIYFINQQEQASDDLASIPGSFRIIFANFHPVHIEMSCLVLEIFAGENGLIYKDNVEDILESFAFYRKDHSLKYRLEIFVRTIFQSDNIMMVVNILGFLRNIQPHIRNELESSIISPYNFTAILKLIEQRINEYNIDACTYESIQNKLIKYQNIDHFFKQKSPFLKNPVLLNESREDQSKTENIQITQLPQEGEAQFYLEKHDEASFQLQQQNLLQIIKEIRNFEFDSERKPSKIIEFSDQKLFDIVKQQSLQLDSYGKFLSILNFMSNLKVRDMWELSEQALQRLLIDEEDDEIIEMPKHDQIYEDYEKAQQSIRDLEKQIKSIIQGQKQAQMQLTQREQEIIKLNEKITQTNSLQQEYLNQIDKLTKELENIKAQTVLKEAQNIEQSKEMENLKNQIKNLEKLNQTIQAAVPSNNTQQIPIQPSNTPSLPPPPPPPPPPPVKSAPLPPPPPPPKIAAPPPPPPPPMKAGPPPPPPPPGAPRPPGGPPPPPPPPGNKAGGPPPPPPPGAPQPPGGPAPPPPFGAPPQPQNQGLKQKQNPNVPMKPVQWAIISQKAQIKDTIFENIKDLDVKLDIEFLEKEFSKKQQTQQADTNSKTQAAQPQKISLLQPERQKNMELVLMKLKIAPNLIYEALIKCDEKILTLPTLESLDVITPTEDEIGTVQSFDGDKELLGNPEKYILKISQLKGFSIRIKALKFSCQYVEYVTDLDAQITSLDVFNDLLSMGWVTQLIEYSLAVGNYLNGQSAKGGAWGFKLEQIEKLTDVKGQDNKSNVLQYVIKQIKFNEEIDLERFDIITKLPISQLNTNLNEIKKGLNMVSKAIECKSNDENDKIVDTLKDVQSKLQEMTKDLELKIQKLDTDYKKTAKYLCENPADASDKFGEKLMKFLRCIKQQKDKIREEEIKLQKQQQQQQQQQNTKKQQEQQQQQKNDIIPSSMKSSQTKAQTNKMVIRSSILQAQRPSMMVNNLRKKSQFQKLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X578</accession>
    <name>A0A8S1X578_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1110167</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000111">
        <property type="protein sequence ID" value="CAD8196171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="310318at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="180"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1271"/>
            <end position="1295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8196171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1308" mass="151978" checksum="5D83BF62E350414B" modified="2022-10-12" version="1">MNSSSIDYSSIRIGSNYVSLSGVNTNICWVLTQNNKLYKGLIKSNGELYMNFLDITISKYYFQINLITNDFGMLSSLEDGELQFTLLNSTSLELTDIVINYQIESQFYNPIVQILSINNTQFTFCLIYTTQKNAHIVYFTYNQDDIEQTSELIFNGSYSNAFLSDYQNYLFLINSENVYLVDLVQNSSIQEIFNANIQTQDDPQPISYFSTFQISNNLLHIIVLQQQILRSFFIDTNYINIQVQSISYILNEYQKINKIELTLDNIFIVTDTFIIDLELKESIKATGYQLIQSGTYAFFYLLRNIVVVQEFFNDQTIISTYLINEPSIFISQDIAEQLESMVLPVKLDIQVMSQLNNKFMEYAFQIQLVQLDVTENQQPIQFHQYIDDLILIFPQDKVIDRSQAFLGPNLTLSISSSNSNYLYAGIEGTRFGEWKVYDATYSIACSFSRQSQLYCTQYINDTIILTIENPASSQQRFNIETHLHLQKCSCIKTAQYINVLMQFKNSVLVQQFDHSLQATQQITLPFQMEILGAEILKDVLFAITIDGKLIATDIFEKQQLFTISNYNFKEIYINPYDFPHYLFIDNLDELIILAYNGKQSYEFINSIQYPIQNYQSNAIGILSNGIYLAFCKDNNNFIYFYQKINIYLQNSQQNYFDVYIEGYKIYQNPKIISTYTSTNFYLIFDQQRYFQLIQFSGTGTSYTALRSSKFSILQGLKINQLSSILKQQPGYQVQIVYLFTNTEYSINHPYLSYDYMLAPRFQENDYIGKQHYLMFRAHNQNFTSYYKQYFSIIYENYLLLPRNLHHKQTYYIQNKGEIKINPRTLFYGNIQNYTANCDECKSFNITQPIAFSNSQSINISVENILNSNDLVYLTDQQNNLYQFYPETNQLSMLYNLSDQGHQQCNFLFIEMQNSFPVQICTGKSTIIYAYNISSNQIIKHNLIEVCCFYYSSYQNGMLTVFSNGKFQGLDYFVFYFWISNNEINLKQIFNSNGVCHYCLISPLYLSMSQNFTNQPCQIFGLISTSIDGSGNNEIFLLKHCISSTMSQIFGFEPNQTITSGNIYFQSSTWYLIQTKFISFSFSQITKQFGSSEQISDNVIQVKLIVSYFNVVELYMVLFNQTTGNLISFVLDQGFDIDFNETVASFQVSQGSNGLLMIESFSKKNTFPPQIYTYEAHKFEIQYETKKLNRMIGFEIIQAQSSEVYSVLSGNYYAIRNKTNSLLYYVQNYLTLSVDLTGIENTSIEITSYNPQNQINQSLFIQNLYYRNNSNNNETPINNNTNIPDNPIIPDNPNSTDSDVXKQLILILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XAX8</accession>
    <name>A0A8S1XAX8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1170009</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000117">
        <property type="protein sequence ID" value="CAD8197941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XAX8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8197941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="152" mass="18419" checksum="B662CD52BD1A22D5" modified="2022-10-12" version="1">METTLIPQIQGQTQLEFWSYYVTYSEVTSFPWIQQEPEKGRWKSQLLFQYDPGDYEGVWKLHVITFDYNIMFYEAYGTSMYINIFFFQVNKKLKNLDIILEDLELLIKDMNQKYKEEGFPNFSLIPIKDIFNKYITKESISQEKRNMKKQQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XDU0</accession>
    <name>A0A8S1XDU0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1210024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000121">
        <property type="protein sequence ID" value="CAD8199145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XDU0"/>
    <dbReference type="OrthoDB" id="422186at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034498">
        <property type="term" value="P:early endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028662">
        <property type="entry name" value="SNX8/Mvp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46571">
        <property type="entry name" value="SORTING NEXIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46571:SF1">
        <property type="entry name" value="SORTING NEXIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="1">
        <location>
            <begin position="165"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8199145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="535" mass="64043" checksum="A9317072F31A1482" modified="2022-10-12" version="1">MDRASFVNQIDSFIETTILAQGYEKTEFLQYLNSKKTNGDKIELWNMDEILIAGGEFVALKQQQQTNTEMTKYPEYQNNHQNGKLYPGFNDYQSPPSYIQQPQFIKQGTLKEEQECLMQTPQPPLPQQINNQQNVMITEVQRYKVYPGQQMQINQNSFLYKHPNIEVKIQSYQEQPGSLFSKSYILYNITLDPYGYFISRRYSDFDKLREYLIKNYPDYYVPPIPKKVSSSTKNKIADFRQLALEKFMNTIIRQFWFDNLVDGFFSCQNDNDLQNKLKQQKQRQYDIQNLTTLNGQIECQISSQIEGFFSYQQGIFNKDLECYNNIRQISKKIVNLNKQLSQEYKLLADQFVILQQRNRNQNEQIPEKVDEIYYKHLNQMFLTWSKKQEDIGLIVTKNLDYFYKYQNQVIETLKEKIKYRDSLRDETLKQLQKIESKIDKQFKELSDSQIQQKTQTQDVFTEKQQKQSLYPKESKQFENIQDNFGYINNNIYQQIQIIMNLNQYQTTQTILNMQQKITQIYLDMTKENEQLFVRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XID4</accession>
    <name>A0A8S1XID4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1260034</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000126">
        <property type="protein sequence ID" value="CAD8200875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XID4"/>
    <dbReference type="OrthoDB" id="309481at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="116"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8200875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="234" mass="28565" checksum="08163A93B547F2E2" modified="2022-10-12" version="1">MYQGTNKQIDQLKQEIEKTNIKIKKLEIKLQNEKKELKEKRILFNGVKIQYQQYSKLEEKQTNNFYKIQEKLSKCKEEEYQKNIELQNDKYQISLLDYEVDSKAFQKAPFTQKQQFIEFREQDEIALNELDELEKEKKRIKNKQKQRIELTKKIEITAPKLQQTERQLRHNQYQKIYYEQMLDDVKYIIEQKQTYISDLEQSIHEYSLILQDLNSDLQTVMLNNLNFHNCSQKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XJP5</accession>
    <name>A0A8S1XJP5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1260136</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000126">
        <property type="protein sequence ID" value="CAD8201077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XJP5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="216"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8201077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="337" mass="38934" checksum="4469C1F1FED8685C" modified="2022-10-12" version="1">MYQYVEGTQFMQNLQNLDWNIGYEQLTIGEQLAQELLTATNIKSVNINGNEIVKGNTFPVVYNEAQLNKSIIQLLILNGQINIYGPQLNFQIIDYQFQYIQNQAFLNRYNLTSGTISFDYRQRIILNSEQLVTGMWNCTSILNLPQNQQFINCLRKFINQITQKGNKRIKFTKQDSFILVIFDVQATCILVSLGDPILQLIYKPKVYRLWENFLNKNSFSSDLQFSINEYDQLENQISTLQNEYSNMLKLLLEPSNQLIDSVPNKTSLLILSKSNHQTNNHNSIASHQFQGLLQVSSQKLFTGRSHLDTKRGYHLSVGQLSETSFLQDIPLINQINQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XL80</accession>
    <name>A0A8S1XL80_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Carboxypeptidase</fullName>
            <ecNumber evidence="6">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1280022</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="6"/>
    <dbReference type="EMBL" id="CAJJDO010000128">
        <property type="protein sequence ID" value="CAD8201488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XL80"/>
    <dbReference type="OrthoDB" id="433625at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000408">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF3">
        <property type="entry name" value="RETINOID-INDUCIBLE SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035883154" description="Carboxypeptidase" evidence="7">
        <location>
            <begin position="16"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8201488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="469" mass="52884" checksum="17E0307D5EAD3494" modified="2022-10-12" version="1">MQKIILLAFISLGFSRLSFKEGPYDYSHIFKSEGTFPQGTKCKKVNAQQLDPLFDDTQIIYSGYLSVYENSNSALGFIFYGSQKAKQESELQNYPTLIWLNGGPGSSSQLGNFMELGPLLMQSNGKLKKNLYAWNTEYNVIFVDQPIGTGLAYVEKQSEVPTNQKQIGQQFLYALNQFLFGAKGCVEQQQVRGLAQSPWFVFGESYAGKYVPTIAKAILDYNLNTQKKIPLKGVGIGDPFTDPYAVIAEYAAYSFNLGLIDIQERMQVDSILAYGLTELNKGNTLAARDAFEESLDIIITQSGDMNVYNVLQYGNYGVTEEKVEKYLNDLQIVNKFGFSEDWKYKISNADDGPVQKALKYDFMLRDVVATVEEVVKQIPFLIYNGQNDLICSTPGTLRWVYDLKYEKIEEYRQKDFDILKITGTERIVGYQKSAGNLELVLINNAGHLVPTDQPEASLQMVTTFVNKHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XM16</accession>
    <name>A0A8S1XM16_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1290071</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000129">
        <property type="protein sequence ID" value="CAD8201877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XM16"/>
    <dbReference type="OrthoDB" id="10249433at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005645">
        <property type="entry name" value="FSH-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF197">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_38, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03959">
        <property type="entry name" value="FSH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine hydrolase" evidence="1">
        <location>
            <begin position="142"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8201877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="298" mass="34191" checksum="B928B24F99D7672B" modified="2022-10-12" version="1">MQLNKLFFPSSKPTYTKVSISLLPNVELLQIQSGNSEQCFRCSVPQNRQVPVLLVKHENFLDDLNNKYIMFFHSNSEDIYNCYEYLYPIISTFNVNLILVEYPSYGIYQNQSASADIISEDAIAVYQYFINKRRIKKENIILMGRSMGCGPACLLASQFNPAALITISGYTSLKEVAQSFIGSVLAKLIDQKFDNLKIISNVTCPMLVIHGQKDQFITCDHSKRLISQTKSKIKQYHLSENMTHNDFSIQDDIIQPLRNLFEKINFIQIPQQLPKDQNPLGIIVLRKQHSKKQTNLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XN05</accession>
    <name>A0A8S1XN05_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1300176</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000130">
        <property type="protein sequence ID" value="CAD8202327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XN05"/>
    <dbReference type="OrthoDB" id="1872379at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="25"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8202327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="64" mass="7868" checksum="411FE25D5C9610B4" modified="2022-10-12" version="1">MNNLKQQENEFKFYNQALEKDWDCYNAFMCRSQYYMDEQKIDDALRDFKQAIEINPINSIVQYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XSH9</accession>
    <name>A0A8S1XSH9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1340031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000134">
        <property type="protein sequence ID" value="CAD8203502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XSH9"/>
    <dbReference type="OrthoDB" id="285661at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8203502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="332" mass="38840" checksum="799D83142ED047F8" modified="2022-10-12" version="1">MGNCQCQSADIQKTEEMQPLEFKQENFKSSEDQQLQFCQAQQILQQESIKSNNETSDDTHRPNPQVVWQTQTQFTTYNLIVQKVMDELGTYEIDDEDGYFYGVYEQKDGSLYRGGWHQGQKFGYGCLIYQDGSIFQGRWRKDKANGKGRMIYTDGDWYEGDWVDDLKHGNGKYVHCDGTIYQGEWKNDFQDGYGQEQFIDGSKYKGQFKNGKKNGFGHFIWVDGQSYEGNFESNYFCGYGKYVWTDGRQYEGQWLNGSMDGNGIMKWPDGRKYQGQYFNDKKHGQGVLEWPDGRKYSGQWELGKQHGIGEYFNGQINKKGQWNYGKRLKWLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XT24</accession>
    <name>A0A8S1XT24_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1340126</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000134">
        <property type="protein sequence ID" value="CAD8203692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XT24"/>
    <dbReference type="OrthoDB" id="311215at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8203692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="584" mass="69836" checksum="26BFF2E31F2A7208" modified="2022-10-12" version="1">MYPSRFINEQIYTNLILKSKQLEKRGQIKEALDQWNQKILDNLQDIRFYESKAAILMRNDQQEEIIKCWDFGIENNKTNILFYKAKINALQKLGRNIEIIECINKWIHLNNNCSLVENEISQISSYKFEFVNKCDEQDWIIKKKLLSLSSIREYIDYTNILKKLGKEEEILQCFDWGIMNNIDDIDFYYAKSMYLESQGKNQEAIDCWKFAIQKNYKSTQFYDSYSILLTKLNKIQELIDCMQFGIEQNPNQITFYDNKVNALIELGEQQQVINCLDYGISQNKYNINFYNYKCRILKRLERYEDCIECRELACELDNLDRLEEQLECWQRGIEIKKDCISFYQHKFNILWKLKRYEEALQLWDYGIQNNNQNEKFYYQKAEALEMLERNEEMLECWDQGIQINPNNVEFYKEKCYALMKFDKFEVVNKSWVSGQEDSNHSQNQLSDNEENSLKQSENAEKALKCWDLGLSNNPHILEFYNQKIQLLQRLGRIKEALTCREQGIQNNEDDPEFYMMLAEDLLSEKNIIESISSIDQGLKRCDDTRLYATKCQSLKKLKSLRKALIYFRLSDSGMYLGKKLKYHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XXW1</accession>
    <name>A0A8S1XXW1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1430100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="12"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8206240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="214" mass="25514" checksum="D8CE6E81EFBD8F3E" modified="2022-10-12" version="1">MNYSNNQSQRVQNQTLXQKQNLQKQPYQKSSQIDNKQFESKQQIKSKADQLQKIEKNAKDFDLMFKELESLINNVHNAIEKFPNQGGKENYFDQNLIQTILIEDREDDEAEPIDQDYKKKKYMVKQISKQEEQCLDILNKQDQNFFSLGIKLENALGQEKMNKAKNIIKQTLEKMNLEKMELQYGPNYEQLLPFLTLEERKKYTQLLFTYILCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XZH7</accession>
    <name>A0A8S1XZH7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1440017</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000144">
        <property type="protein sequence ID" value="CAD8206405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XZH7"/>
    <dbReference type="OrthoDB" id="308574at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="296"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="244"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8206405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="700" mass="80778" checksum="822F468FB3CE0C8C" modified="2022-10-12" version="1">MSLHNYSICNLPDHDDEYLNIVCIDEKCTNRQLLCCNCIQEHSKCKCIPLKSFLRQFQNQLNQTNLDTSKLIYNDLILHFEQIEATLEQSQKLINEIFNKTKSAILDAKSSIQQINTSDTLKQSQSLILKKFESEPSKENFSKVLAEIELFKPNSDKYSFTVKKSSTQLLPQSTESAANGKIYAQQYQSSANTFTEQFLQLHEVLKKNITNFFINAPTRVTEQEIDGKRFDRFKSHNIFTNVSLGNQQQQLQQQQQLQQQQQQQQQQQQQQQQQQQQQQQQQAKTIIQIEEDQDPFETSKEIKSNQKNKQNNSLHMAPEGEGTTSTKPIQLTGKKDAANTELQKKHNNNGIAQQKKSKQDQIIQKQPELPPYKAISQDDNLGDGETILNVQELKEFQFMSAGQEINKMIYLNQQMIGGIGGSKFMVFDVSSQDSEKPQRIHTIQIDAEYTDLAYLELDDQNGQLYLATKKGNIYTYIKEGTKNITFRQSSSQIHILDKPGVLYIQVSVADRLLYSVGEEKIVKVWSLKTMRELKRLEVQDLPTAFHLDQKTAFIGGSNFIQIWEQSTGTISTMKGLESKVTKILTNQSKMFVSMLEKIKVYKKIGMGEYLLNHELHLRQIITMQVLRTWPMLVISYIDDKSIGRVSLYNYVDLQPPEMLMDKYAQSCAVREYNKINYLAFGLQTGVCLIYKMEQRFQQSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y0F8</accession>
    <name>A0A8S1Y0F8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1420062</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000142">
        <property type="protein sequence ID" value="CAD8205912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y0F8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="301"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="195"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8205912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="576" mass="67478" checksum="F8EDFB157DE86523" modified="2022-10-12" version="1">MSFSTTCEFDHSKNIHRKFNTLHISSRKHIDPLVTTKPFETRNHGQQFEQHFAHYLKGDLNLQRLIQTLNYPRLNAEQQLQPKHRMTLKIRQQTEQRKNYLKSKIVKVRNLIKEKVAIKKPHNEEIRQGNAQTLTIVQTILDIANHKENSGGYQEKLIPKTKNLKQYRLNYDNVEPVCKLQQQVIQSIPTRSYLKSVLENNAQLYLQELQDQAEQHDNNINKIVRLSIQTKQNDFTLFHKAAKTDRRNQDGILTHADVQLYDYLYNKPKGVMIQKHPKYIQNVIDSEIDFRKNFGRKVLTHRHKRTHYNQDPPDNPDDSCASSLQSACEIKLDNYYAETREWKRKQYLQLPRRKPSIRQLINQKLNDNKHDDNLIFNVQQIMFLKREAPQSANKFNSNRKDTRAQVPSFENLQARIRDFSYSRLSNVVATQPQRSEPTYSPYKAQGLQQKITDTLGPRTKSFNSGHKWRESFQSPSISSQSPFKFVQSPPQIKYSIETESYTKPSDSFKYDSFRTSSEQLKKRQGRSLADVVQLNEIVALRNKIESSYVSRSSLTSTYVSELVKLAQAITTTLKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y1E1</accession>
    <name>A0A8S1Y1E1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1460040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000146">
        <property type="protein sequence ID" value="CAD8207077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y1E1"/>
    <dbReference type="OrthoDB" id="305556at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8207077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="554" mass="66613" checksum="33827AB858C0C236" modified="2022-10-12" version="1">MKKEFKKIQIHLFEQYQKIQDKLLLKIPYCNHFKIAKIFKINEINQGDSIKIDYYYDGLNGEQVKIIMKESEGKLEGFIKDFKLELQDVLMAKQVLQCLIESFHWTANFGQFLDPFRENLIFHQEQDFFLLIPTFTVITKYIFYNKFEFSWEKFVNYRLNVYQNVTHIFFCEFLDTFYPYVKELDEDKKFYKQQFYAFQLKRQNIDELFELKEKYAGVDSLYSEDLIKKKSWRYSFKQSMILYQLDSNEELRKLFLDKKKIDQELSKNGKQQPNIKTQTENLLEFLRALPDIRSESHDVLTSFAQKIKQYLDSKEEFKQLKNNVAAVFRPNYKHVVNNNKMIDFAKQQQQEKQKIDKQKIDIEQNNGYFDFVWQSDINSLNIISQYIQSSFNRNGGDNQCSMKLGAEGKQLILKYKFELLEQKNCYIVRTVIPEIMFTIETADDIMFLVNVIQKEQGQTNLYVDDNRRLMLYSSIVMGYKIEILSAIVKIIDIHQIYFYLILEFYAKNIAVGRSVRELKYQHFVRELLHYVQDYENDFENQMKNIMSDNVQIGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y3C0</accession>
    <name>A0A8S1Y3C0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1430092</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y3C0"/>
    <dbReference type="OrthoDB" id="5337378at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="12"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8206224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="569" mass="68161" checksum="17803A12208776E7" modified="2022-10-12" version="1">MASNIKVSHYSINTDEILGKGSFGIVYACQSDNFKNKDLCVKIIHYKAQVFKEIQLLELLKKTSNPNLIMIYDIIQDQNQIYIFMDRCRGGDLKSLILDYSIRKKQFSLEQIVDMIRQIILGYQTLIQNKIIHRDLKPANILYEFKEKDKITLKLTDFGLGKILDDINIKQYMTRVGTPAYMAPQIVFGDKFSTKCDIFSLGIIIYELAFLEIPSQGMNPFIRNQFQKSLKTKPFVCPDLCQQQPDYLKCYLQDLINKMLSFDEEKRPSWDELLKSDIMTIINEALPIETKLKNEEPNLSIIFLKYERQLAYQKQYDQIQFCKLQEFKYKLQKKDELLRKSYLFIFMMISKAQLVMNLQYQFQLIVKKQFPNFDAYHFQLIQTCFIGYIYKILENAFGFCNQQMDQINEIFKQQFQNKLLQMSQRFYQSNDIEVQEMKKFTYHFLLQAKVIFQVAENDLINSKAKHQYPKNIEKFYRLISQKELTDFEIYAKWFYFFWNQHLRKFLQYDITKQNDIRYQSLLILIEKFLNLETDYPFSKYEKFDESQVLLSENSTKEELDKSIKLRVYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y7L4</accession>
    <name>A0A8S1Y7L4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1540053</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000154">
        <property type="protein sequence ID" value="CAD8209303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y7L4"/>
    <dbReference type="OrthoDB" id="283021at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34560">
        <property type="entry name" value="POLYKETIDE CYCLASE/DEHYDRASE/LIPID TRANSPORT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34560:SF1">
        <property type="entry name" value="START DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8209303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="327" mass="39238" checksum="4C1F8A8E02DAE51B" modified="2022-10-12" version="1">MSIKKHLLQLPNLVIEEEEEPEFDCIIPSAIKLIQIEISPNKQQLSELQQLKYLIDDKQYFSAYQMKQELFHSYSNDYEYLNYTKDIFELIDDDIEEIKELTKWFELQDGWSIQQQDSRMQIRYQQLPNSPRVVARIDTEIKIPLDIFISLIYETELYPQWFPFCKDSKTILQPDKATKILHMHSSPPVISDRELCVKGWGVNNLEYDGSVLILSHSIDKRTKLLKRYGYIYQYIFFEFLFQKNQNMLELIQMYFVQKLNQYLLIITQQVQQFVLIQNQNTYQTSYQIICLENLQVLCLINQYQIQKNIKDLYGKRKRMKVQNSMIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8K4</accession>
    <name>A0A8S1Y8K4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1490005</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000149">
        <property type="protein sequence ID" value="CAD8207864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y8K4"/>
    <dbReference type="OrthoDB" id="6612291at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000818">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF12">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="196"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="231"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="316"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="350"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="383"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="407"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="446"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="473"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="71"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="17"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8207864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="512" mass="57831" checksum="DFAAA2C030484335" modified="2022-10-12" version="1">MTSQVIPLQTQEDENLNSDNSQSVISKNSSVRQQPIAPVPMPSNKLRKRGTVQLENEVEEEVNSVFHLSVILVAIQSGNFSLGYSLSYLSMSFTTLFSQITLKGTEIEEQGLFSAVLSIGQVVGALMTQPLLKYTTRNQSLLIADICGILSILQVVPNREVILAFRFCYGVCLGISTIIMPVYVKELCPQKYYETFSVMAGFLVAGGLLFTNFIGLGYINDDLRGEQSYYWQIVFAIPAVLHFLRSFIVTVIYKMDSPVGLVQIKMPLSAKQIIEKIYQPQFIDQVMLKCQLRVQYNEEHQEGLLSLFKKKHLKTLTIGCVLVFVYTWCGLFALFSYSSQIFGIMSDGDITLNTIFTLIIGLVQFTPAFISKYVYGRWGKRPLLLYGLIFLIVCQILIIGLSYSDALAATIVKFVIICLFAFQYAMTLGPITWSMTPEINSSEGTYFCFVTLYAWQLLVLYIFPFMLDGIQMSGAFIVFGILTILSTIFFYFYVKETKGLNHKEIDRLYGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YA84</accession>
    <name>A0A8S1YA84_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1500125</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000150">
        <property type="protein sequence ID" value="CAD8208404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YA84"/>
    <dbReference type="OrthoDB" id="283324at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF8">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0810">Translation regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8208404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="202" mass="23914" checksum="1A674EE78FF20378" modified="2022-10-12" version="1">MSDKFHKLSSKWTISEKYNASYNIDYKRTIVDICSFSTVEELGFLMKKTIYSKLSDILSEPQKCKIFKQFNDENADAQIEAIQFFKNDIKPWWEDENNKNGGEIQFDISTQYYAVYDQIYLDFLLQIIGQATEELRHINGLRVLDKINAKQGNRIRIELWMDIDPKDQDESIGRLIEWIDSTLKKHKIDIDQTQFNKVSHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YB88</accession>
    <name>A0A8S1YB88_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1610044</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000161">
        <property type="protein sequence ID" value="CAD8210881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YB88"/>
    <dbReference type="OrthoDB" id="3045089at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047126">
        <property type="entry name" value="RNF141-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109">
        <property type="entry name" value="RING FINGER PROTEIN 141-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109:SF5">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="289"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8210881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="355" mass="41854" checksum="FF3DEB9F22D25697" modified="2022-10-12" version="1">MAYNFVIILYYTDLQYYIFGNMFLLLSFVIRTKESCRNQITHEYSSIIRFLKTFYRFSSLVASVCISLKLSKYVEWTWPQAFWWYWMFLSALVGTCFTLIIVLISKIINEIFKQYQTVPQEYKSLLWALYISIISSMLSVMWIINTFNKLGLDLPIKIGEVLFYSTISFNFIIFGAITKLLWDSIIDFCVYLLQSQQTEIESQRELPTCQISQQKQYERKKTIQQIFLKKLSSAFFRQTTKSDLIQQSQKMTELMTERGQTKKILQTVEIESKQLQENKNKINESNQKCIICCDNPPNAVLMICGHGGICYKCGLEMAQKSKECFLCRQQILFIYEILSFNDDLMKVVTITKFNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YD55</accession>
    <name>A0A8S1YD55_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1620054</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000162">
        <property type="protein sequence ID" value="CAD8211128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YD55"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8211128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="88" mass="10899" checksum="D9931A789D99AB1A" modified="2022-10-12" version="1">MGIVYHTLQSKQQYQQLCQIPVKGQREFSYHLFPSVFIKSLNIQLQKFEICQYTKNHTFSVKQYFRLQMGLNYRVPKQIYKWNFERQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YEX8</accession>
    <name>A0A8S1YEX8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Translation initiation factor IF2/IF5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1630077</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000163">
        <property type="protein sequence ID" value="CAD8211427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10250831at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071074">
        <property type="term" value="F:eukaryotic initiation factor eIF2 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005092">
        <property type="term" value="F:GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.170:FF:000002">
        <property type="entry name" value="Eukaryotic translation initiation factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001:SF7">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation initiation factor IF2/IF5" evidence="6">
        <location>
            <begin position="17"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8211427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="120" mass="14196" checksum="B0F7D2852E16FBED" modified="2022-10-12" version="1">MIKTVLVNIPSYIEDSNYRYKMPKPQLKIEGRGNGIKTNIMNLHEIAKALNCHDQYPLKYLGFEFGSQTSYKFKNDNDVTCIINGAFQDDQIRKQLDKFIEKYILCQKCRYPEMYMKIKX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YGG4</accession>
    <name>A0A8S1YGG4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Calcium-dependent protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T1590100</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000159">
        <property type="protein sequence ID" value="CAD8210504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YGG4"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000771">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000660">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="11"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="331"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="402"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="438"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8210504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="472" mass="55573" checksum="D652EDD2088D1EE8" modified="2022-10-12" version="1">MNEKTIDKYKLLSTDLLGAGSFAKVYKGIDTTNNRQVAIKMLPKKNILNDKYLMEGLRRELSVMQKLKGNNVVRLLDTLESQNNYYIVQEYCKDGDLGTYLKKQQYLTEVGAIRILTDILNGFIELLQQGIIHRDLKPANILVHQNTFKLGDFGFAKCISNFQRDIMESVVGTPLYMAPQILMRESYTSKCDIWSIGCIFYECIFRRTPWIANSVPQLLNMILKCPVQFPSYISAEAKDFIQKCLEVEEDKRMGWNDLYRHQLIYPKFKDHVDKVINMEAQAEFIINELRQIIVVKQIDLLETFKKLQIQDNQFLTFKELQLLLNEIDPKLNRQQIEFVFNMIDVDMTNQINFLEFQRCLQQHRVRLSLQEIKPDIVYENSPLIQIPKWKIELKKIKDTIEEEQKSLVKLFKQFDTDINQVLDLVEFVKFLRFFNSKINNEDASLIFQYFDKNCNGGIDFLEFKSTFESQIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YHC6</accession>
    <name>A0A8S1YHC6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidase C51 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1660063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000166">
        <property type="protein sequence ID" value="CAD8212057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YHC6"/>
    <dbReference type="OrthoDB" id="299748at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007921">
        <property type="entry name" value="CHAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051705">
        <property type="entry name" value="Gsp_Synthetase/Amidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30094">
        <property type="entry name" value="BIFUNCTIONAL GLUTATHIONYLSPERMIDINE SYNTHETASE/AMIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30094:SF0">
        <property type="entry name" value="BIFUNCTIONAL GLUTATHIONYLSPERMIDINE SYNTHETASE_AMIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05257">
        <property type="entry name" value="CHAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50911">
        <property type="entry name" value="CHAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase C51" evidence="1">
        <location>
            <begin position="35"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8212057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="206" mass="23783" checksum="3AC36ADAF297D727" modified="2022-10-12" version="1">MNKQHPPWGSQIGVAHGGVIGYSNSEPNYDFTKPHLYEVHYKKEESGLRCDIFMGYKYQCVEFARRFFVLNYKTMFTDVQKAPDIWDLETVEDLTKESGTFPFVGFKQGGNEPPKFGDLLLAPQSEHQPWGHVAVVVGVGDGYIDLAEQNYEDAGWITETYARRVKVECKDGNYFVTYIRVGQEDKFNDSWDKDEIIIGWKRIIFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YI28</accession>
    <name>A0A8S1YI28_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1740001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000174">
        <property type="protein sequence ID" value="CAD8213091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YI28"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8213091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="166" mass="19815" checksum="F7C4339B6A6FA637" modified="2022-10-12" version="1">MKNKLLKALELNFIISIIVFTKQMIYLISKAYFNYPKLIFEIQYQKFYCFNSKTGIREIISSIGYLVSLITIISQNLRRNNHKIILKDLRNKQHDDIILTKKVLFLIVLTIMQSCPLIQCILLTCILIWYLGQIINIYFTRNKLDLYIIIWMEALVMVFMIYTIIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YJ06</accession>
    <name>A0A8S1YJ06_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1800004</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000180">
        <property type="protein sequence ID" value="CAD8213483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YJ06"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="149" mass="17390" checksum="E013F22BB8753A62" modified="2022-10-12" version="1">MMSECHKQYKTYLEYTQIWENKCATLGACTSYDGININADDNLLILKNLYIFQIYYQKIRAQTCEDNRNNQSGCAKNVEDCLYYQTKSQEKITFALCTLPEDQASDTENKDCVKELPIQETINANWIVQNVQIENAAIYQQPTQLLHYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YJ94</accession>
    <name>A0A8S1YJ94_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1620103</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000162">
        <property type="protein sequence ID" value="CAD8211224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YJ94"/>
    <dbReference type="OrthoDB" id="283235at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8211224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="341" mass="40406" checksum="CE2D7A7D216EAC9A" modified="2022-10-12" version="1">MSLIVRQRVGMHDESLTHSLQSKNIFKKKQSSSKCHQKKKLICLVSVPKSFKEHVYIKSNVELKLALSFNPDNCLGALKETPCTYNYRVSRTDQLDDVYFYTLELLDQPNYSIDQIIHLLSHKQDSHAIKAAELVQERHKILQEYTLQPYISNEDLLEVEKIAQEYPYQCTIQKVNKDSSSLSQRIVNEQFYNLMGVSREMMLNHLHETKTFPSIFDLGPSLKLWCDLSSNSIQQFQSFETYINTYEGAQYKCTIEQRQMFKRTQVNPNQIVFIEFWLFKVDEPLKSFLMNPQRIYQNQIDYFNKKNTQKEYEAFLQSMKYKQTPLYKNKNPCGYKELTWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YJU4</accession>
    <name>A0A8S1YJU4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1850012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000185">
        <property type="protein sequence ID" value="CAD8213748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YJU4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="185" mass="22707" checksum="B49D81078D6E1809" modified="2022-10-12" version="1">MITIKDFEQFQPQASNHQQKNIIKQQKYEFWESQYYKSILKKFKIVLTKDNEIQYFVDGQLQKIKEEKSYDIELEGWNNLEQIQNLEWLGSYGQKNQKVGKWKATWIGETLHNVVNGTQMMEKIRIMEGTDKQLSILRYLHESISLRIWRIFQRLKVKQLGLFIIIKKDWWWKIQFERLKEWQMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YL08</accession>
    <name>A0A8S1YL08_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1980004</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000198">
        <property type="protein sequence ID" value="CAD8214158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YL08"/>
    <dbReference type="OrthoDB" id="289272at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000210">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="285"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8214158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="619" mass="71809" checksum="FB90230E3A5CD9D9" modified="2022-10-12" version="1">MPPKKQHHKTQSSDISAQAIINFLSNRDPQNGRQSPTKQLDQLKMKTQTHQSPQPVKVTQQQQTLLQNMMRQSQRKLQQPETTKSRIGLMSPKVLSQIHMKTQPHSHQNSQVNLKESMQQLMSIPLSATSKVVSKEKKLIEELLAKTKNSKQQQFFPKSPSIEQKFQTHSGSQPLIFLEKFKPTTARKQEMMTIVLQYKNFRQKYAIDISMNKIQWLIDFVKQELGIPIQGIRTTGISIPIDFILSKPEKPLNLLSGFPIQPLQIEPIILNPNEPKAARVTLKDFEFIRCIGMGGFSKVYMVREVHTGQFYAMKLIEKKPILQQNKQQIIQQERDIMSNLDHPFIVGLQYAFESRKYLVFVLEYCFGGELFYLLRKVKRMNEQEAFFYFAEICLGMKNLHDNNIIYRDIKPENILIDFDGHVRIADFGLSKPGMVDQEVAYSFCGSPEYMAPEMLLKSGHTIQLDLYCLGALLYELVTGLPPFYSRNTDEIYQRILNAKLTFPQNLSASIKDLLNGLLAKNPKKRIESIDTILRHPWMIQWGDKNLYKDLLSKKIEPPFKPDCFAFNFDEEEFGKGEAEFLQYIKPLQQNVMENYPKDIILKNFYYNCMQESTGGTMQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RTU0</accession>
    <name>A0A8S1RTU0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0010024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="289941at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1669"/>
            <end position="1693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8131428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1759" mass="207663" checksum="18BDFA5AE8938A2A" modified="2022-10-12" version="1">MNASYNNNTPVFVIAPYYESYTIDLFNSKDVLENTSFSNVNSTNQNLTFQITPFIETSQEPIESPFCPVDEWGPLYQPARFYLSQTAIELLFYNQTDDKIVQVQNKLYRDLFDLNENFKNISCYSIFQLRKNNQFGVDCDINKNATILIFDLVFDEQTLIIINFTYNNYYQYIYEDEENENFLKKCQYRKTINANILDVANEIKFEYLQYCPSLFNQKAITNEFLIIYHERFNRHNQILELQAFKANIQDLNSCKMEQNTMIEQIFINSLINGFDNSTWNIGFLFQSSPTICKCNVQEIYTSHYNIKCNSTLIELYQPNQIRLVQNLNDEVVLVTGTNIAILVIYLERSYQSENFFTISYKQIFEPGYLLVDMSITSQYLAVIMTDSPFVQQMSPFYSKPNLYCSKQASGETKNILKIFYLNAEYKTVYYQTNSFNQFQSFFQSLNQIDFGNFILLYNFFDKKLNIITLFEKQMNVSYLLGQDNLLQTYNQQIAPIIISFTISGGYFVYDQFQDTYNYNIVTFPRYIILCFIKLEDSSFQLIAQVHKLEQKEQNSLEQCPISKDILVFQEIINSIDLFEGIMGPFLNFNYVPNYNISRFYPYYQNHKAVIKQNQFYDKHNQPRSVFNVLSAVNKNIFVTSNIFGIVEILLTYQNNNQSIEKLETSQSIIFNKTCIINAREYQSFEKQFPINIEIQQCTEVRYRDLHKYLQQVDYYKQFEYYDIAVQYNNSIIVICQKIETLSGRERCQNITDMQKQNELILQIVILQGPIKNERVSYFFTILYNNTQKKLYFYVLDVFLPSQFNPDITYHNCSCYNTLETTNTIISFRYTYINQQTLFVLFSSSQIVVYTPGPSKQTKDDKLYNFTSTLTNVVLNITQCQNNNLLGFEITKYFQSKLAKQYLIVWCQENQNGNFTPYILQYIITNINQINFWRRLPHYDTILSSKTKILISGLILYVPVITKSVYNDYFQNYYIYNLEDYTARSLVEKIEIEKKKNELIITYPISNLMDSLIAGSSISLTLIITEKKFEIYYLNQKAEFIFHLQQQFIPVYNNSPFVLVIGVFQPQKTKTMFRNYTLIYNFTSTQISFAYDSSETYKVEINKGQYIEAGGFYQYLFLVNQSIFNGPIASYNHSSNQVNLSIIPSPYLQMNKYLGSYMNEVSQFGEITQIIDVGDEIYTSLFNQQIKNISNYNELIIVLTDYYVLFYQKNSIPDENRSCIYFDYTQNQQTTNSSFCSIRLIYYHQFKPFNSFLFCQMQLNGVNVLIIVCQLKYFHKLNGNLSFQDKENIQQYIYLEYSYTKLTNNISITTYVMNNRQFCFCYFNDLQPFLYNNQVYQMIKIGYQQLNSYFGIYQIIITENQTIQFIQYNSYPNQNNSQPFQYSEDFALSIIEYQQILFLYLIVLQPNNIFVQVINLTTGFPAYGPGKNLIFQEDEDYVRFEKAVFIQAINHFQPHIVNESQMDQTLSVGNNNQIYEITFNVITFQAKLQYTFYQYLLCHHNFLSRPQIELSYQNDTVFYIVSQCLSNQFEYQSVQVEMTYLLNELNTNIVYTSLFIRNYSRNYSRPVEILEPYLVNPQELHFQKIQLYQSDAQENTSKSHLLITNSTYFLIDLILYAENGYYLYYPMQSANDDRDHSVYLNITAYNSYSSCQAQFEIGWIQQDSQINNVAWFYVTLSILLLAILIVIIVVIYGFCQKQSQIGKKDQNLPLERIGDDESEYGVELQGAGYNQLMLGDVFKDLLRDNKSKKQTISYTDELLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RU21</accession>
    <name>A0A8S1RU21_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0010083</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RU21"/>
    <dbReference type="OrthoDB" id="6154712at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052779">
        <property type="entry name" value="WDR62"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45589">
        <property type="entry name" value="WD REPEAT DOMAIN 62, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45589:SF1">
        <property type="entry name" value="WD REPEAT DOMAIN 62, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="330"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="443"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="685"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="748"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="823"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="961"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="685"/>
            <end position="698"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8131486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1059" mass="123328" checksum="B6D1CA909AE525E3" modified="2022-10-12" version="1">MNYIIGMSVTQKHQISIQENLAYAAGSIVVYYSYKENQQEKYLIGKAQVHAILISRDGTHVFTGEQATKNPAVNIFKLTDEREINISQQLKGHKFGITQIVESPAKPYLVSLGSEPDKGLFVWDWNQGIKLTVNKLSKPVNTIRFNDNGRLLISAGQGHLKYWQFKNDGSIIAYDNMMDPKPFLLNEQFMHKNFIDIHVTQYSVFALTFDSLITVFSLQTKQFEKWMDLKATNSYSLTSHSNYLITGCSDAIIRIFNLNMQHIVTLSKPPNLGNYNIEKGITKLKVQGDQFADCLAVQIYQNYLVAAYSDRTMFLWDIGNFEKIVVKRSFLNHSASINDLQIMTQQSSIEATFFVTASSDQTLRVWHIYEQQPLGLRRNAYCKYLSKIIYIGDQYQHFKANQQAQLQIRCVRVSKDGNYLACGDSAGILRIFSTQTFTLYKQIQAHDQDIMTLDFSEYIDLLATGSRDRIINIYDDQFQKIGSLEEHTSSVTCVKFSKNKLISCGLDKSIIFREFNGQKFKIYHQEQISKIYSMDVSFNNLYICLEKKISIYDIPTGKLKQSFETDANSKILLYSKDDQNCLQIATYSDKNIKIYNNKFELIQKFQHDQLTSMGFAMDNKHFITAGHEGCIIIWKLNKVFKKKQLGLIGKKLKQVFAQQDLKSSKEWTINSLWIEDELRGFLNNNKQQQQQQQQQQNQKQEEEQVELFKFESDNEMDPKSSKQIENALSPLGNSQFNYKDQKFDKDDIKTIQNQLLIVQNNLNKVNEKQQNKYQIHNNQETIQEVNQLEEQSQLNFFYEKSQYQQTSRSQSVDSQILKKRTREEIKQDKLKQLNKKHDMLQQNLITQESQVDQTQRDTTVLETKIIEQDTQIINSQLHSNLNSYADYQQQQNKQFIPTEASLQINNATQTQEHIKQIIGPISKDSINLNNSYAYDQHYHQQLVDPTQQQIFQQTNLARCHIRQIQVELLDLTNLLNQIKDQQVKDNVLREIDDDLNNLQKSIQLIRKQEQQEQQTNRIMEQSQAQLSEEDIKKIVINCSKEMFENYSKVLFEQIKGKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RW26</accession>
    <name>A0A8S1RW26_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Vacuolar protein sorting-associated protein 8 central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0010485</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RW26"/>
    <dbReference type="OrthoDB" id="289259at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045111">
        <property type="entry name" value="Vps41/Vps8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025941">
        <property type="entry name" value="Vps8_central_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616:SF8">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 8 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12816">
        <property type="entry name" value="TPR_Vps8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vacuolar protein sorting-associated protein 8 central" evidence="1">
        <location>
            <begin position="481"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8132186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1234" mass="145478" checksum="63E9ACD45D8E31FB" modified="2022-10-12" version="1">MFKFVEYKNFEQFHQFLNSHPQITYQEIIKHPLQEYQFENEFVNPIIVKQSVKLPFQEYINIMHCSNRFVYLGSQKRIQVFDIRTEFKLIQKIEIQNSLVIQETEDCLFIGQLEQILLIEISKDFSIIGTYKLGQVQVPFSFLKVINESQKQLDWYEIVIADLKGNSSKIKCNIFKLFNKRNVQFSIQELTQNGSIIDQKGKNFIDSGFSILYELLIMQGSNENYAFLLYQMHNRISIIRVNKINKETPIQLYNLQNDQLDAGWACLSTCLDNNSQIRNSFCCSYGNVIHLFMYEIFGGSITVDEIGVKQLETEKKLNGLECCDSDIIVVLFDDGFALVHSTEFKKCENKLNYFNQSLKIKKFNGNFMLGLTEDTFEFMALQDCRTLLQNQADQLEFRKAMQNAIRIMNGDLLMIKIAPSDQLSELLQKLAFTECLFLGKQKNLTVQSLSEIVKFLIKAKLEHYLFTIISDVTSDLGYKDVFVNTIESLLRQHQIPYIPDEQLYELCKMYQNQLNIDMIHHLVCSTDFNRISSAILITCCEQMKMYNEMIYVCQMSQDFVTPLVKLFGGNQLQEQCQKYILSILEGKSFEGKNLTDEQFTIALRNLIQFLFVSENLLKFINQDPKGAIDIIYQFYIGKAQACIENSRAEIILKLQVDETETTLFDFDQNNVRCSVHRKMYLSVRKLEFIKSDIWISYLTAKLLIQKFPFSSQVAYDTIYTLCRNYEFLLSLNNDILLINQFIIRVMMSKKLTEQQLSDLEFSASIVKKFSLALVFIYSQQQQYKKAFEAFKIVNSQIRELIFPWIQQLVELRPMILNQQNDEFILEILKYLITIDEQQTYKLIEKCFHQKHFQILSCFQKDFEDQKYFANFISYLFQSQKVFLINDQTKILYLKRLCEIQPKQVLQAIKSMNFPLDEALKLTKEYKINQAAAYIHQISGSIKQALDIYINSFLKKIKKSIVLLMKKQNLDNNCTDKLKYKLENIIQIILEDLKQNQSNYYDDQNLWIYFVDLYINQNKIRTFIMPNIPQSVKDLLSLYLSEYLARISDRVQVSQIFDLLNLKYPELPVSVFRQTIYRVVSKFHDDLRTLKETQLIQSNEKYQIIQQLKEINQSAWICNHICQRCQNHIVDSELAIAFTCSHVYHDYCVLKGIYKIPFCDACQSETSYKIQKERILKQYAITQQISESKFLQQMSVNKITVISNQQIQMQTRKDKKQLIKRFDFELQQKYDYSWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RWZ2</accession>
    <name>A0A8S1RWZ2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0010635</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RWZ2"/>
    <dbReference type="OrthoDB" id="310155at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="473"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="600"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="441"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="479"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="600"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="630"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="649"/>
            <end position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8132486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="736" mass="82170" checksum="A1D4FA06BAC3B97C" modified="2022-10-12" version="1">MNIFKQKKKEEPKKKITVTIEQLTMLFMKSKEYINLDKQKKINEAIQQETKLLNMLKAPQRNRKDEEIQIIKIVNILNTIEAYDKAIIQISNIDQFKSQIVASQGDPSKVSQYIPYIASVCYYIEKTNDLRGAVDLKQQLNEYYGCDIATTFEKSVDQNAKYLFGILNLQSPSVQKYAQDFSKKYQVLDVPGLNYQSQQQQQQAWNPAQYPQPQQGGPQQGGGYPQQQQGGGYPQQQQGGGYPQQQQGGGYPQQQQGGGYPQQQQGGGYPQQQQGGGYPQQQQGGGYPQQQQGGGYPQQQQGGGYPQQQQGGGYPQQQQGGGYPQQQQGGGYPQQQQGGGYPQQQQGGGYPQQQQGGGYPQQQQGGGYPQQQQGGGYPQQQQGGGYPQQQQGGGYPQQQQGGGYPQQQQGGGYPQQQQGGGYPQQQQGGGYPQQQQGGGYPQQPQQYPQQGNYQQSFQNSQQTSYQQQIPQQNNFYPNSYQNHYGLQQSSNPYGQLQFSQQNIYQNNPNVFLPQISQPQIGLNQKQNSNFNYPQQQHSIYNAPSQKQDNNNIGFVPLQQQSQFQQQQNQSQQQQIVVDPFAIINQSNNQQMVELPPLPKQSTIQSNIPLNQNGDKFAFNNQAPSDEQVPFQSQNSQNQNNTGNFGQNNPLGNSLNSRGTQLTDPLSDQNQNIFETTTIGQDMAFSLPIMNNKKNEEVPLGINHDLRSLEILHNFKNGVPQSINDSINQLQLAGLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RYK3</accession>
    <name>A0A8S1RYK3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0020229</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RYK3"/>
    <dbReference type="OrthoDB" id="297037at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8133098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="495" mass="58268" checksum="8584D7754E88F520" modified="2022-10-12" version="1">MNKYTLNFKDKEIESNYQNITQQNFRVFTLTIMTLGLLVVALTKIIESILLNEYQTIYKYCLFICYLLIQYGIMKKHTKYIRFAIILLNHLISLFFSLQVSEQDDSYNSFLKGANVMGANFLMLISGEFMDAAISVITIGIMKIILVQIASNLLLFSTIISSVLVILYTIRYLYHFHKAMRNQFLLALNDSHWEKILNSIAFKQPYIVLSFIEDTMQFTLKSEAQCNHFFNRQFDGSNFIRDSIYKGNKLEKFLFMQIQKYRVDRASIFNKTLVVEYLRKMIRIECSIYYGNKPTILLLMRDFLKQKQPDTSIYEIRHRNFIRLILKILSHKDRLSLLKCRLIERKLLILQLYEDLRLSKLNESEINIYNLAQIIHNLYTNLSVKAFVTGNSNINTIQNIFLLILLIIAENSQGQQLSICLTNEEESQRWLHISGNFQIEKVKKALKSISDYIKLISQSQIMEQFKIELNLNQYILIPFQVNQINARSLKHIDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RYL3</accession>
    <name>A0A8S1RYL3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphodiesterase</fullName>
            <ecNumber evidence="1">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0010679</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="1">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RYL3"/>
    <dbReference type="OrthoDB" id="286557at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="2">
        <location>
            <begin position="559"/>
            <end position="895"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8132572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="895" mass="105432" checksum="B16AD3C493718051" modified="2022-10-12" version="1">MRAIQELDTLLRETETGKIKLSLTLKDLAQLHHLQSRLQECKFLNSIYSEESRIKQYLSRAVTVNLQIQEELCQIIETKMESITSNFNHLDKLILYILNQTMNKTTMKNVLRQFSNIILGSNNFTLLRFQEKSVESVSTDEFIEQQLTEQQKLQLYQQFHGDQLEIITFQPTGQLKQIFKCSSLQIIRVPQKLNAQTHSSLIIYETQECAKLPLSKPHQIKLICDQLITIGYDYLNENYHKDDLINSVRAIGQFLIKNAPILFQECIKIEIERKLNTKCEIIQSTKHSKDTSFAILENQTKCFIPVMSNHVVQFYLHMENVSLDIITFADFMSQLWQHFKHSLRELKDKEFLYQIIEVSTPTKLVIASDLHLNIIYQSGSVPSSWNFLDLSNQQIDCYQNLTEIKMEENYKSMLLSSLNEVQKSKKGAIDAGQIIYHDKYVDIAIYVKKDEKNEINQFFIVFDQPKKRFESQRQSKSEIKVSETLASLKQEKIEKACLILQEQLNLNLQTEEIIDMLKEHALMTIIEQKEQITEDIRQTYVLEYYRNSEQVQTYRRTISEPDTPFATKTLIQEQQLTNAKKMELFQLRPDDLENLNNWCFDITVQKDQIKHYTWALFHLLNYFDKYQMYKETFFQFLVAIEERYNSRRNPFHNFEHGFTVAHACYYMIKNKLLDEYLDQTEQFAAILSSLCHDIDHSGRTNGFEVARMSKLAVRYNDESVLENHHAAMTFKIMQREKYNILSNLSQEQFQKVRRFMVSNILATDMKKHFDLVSSLEIKYKNGEMNIINMETKRILSGLIVHTCDLTQPTKRFEITKKWSIRIQNEFDNQVEEERLLGLPVTQHLVCQNLPKQELSFIRNIIQPLYTLTSSILNNGLSVALKCLEENEKEWTKLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RYX4</accession>
    <name>A0A8S1RYX4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0020292</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RYX4"/>
    <dbReference type="OrthoDB" id="311528at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="407"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="407"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="424"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8133216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="517" mass="61103" checksum="7323BF6EAAAB8C1D" modified="2022-10-12" version="1">MADNDDYEIQYYKYLEKIGMTPPNVKVRQTPQKQNNSGISQRLLFQNSSQKKELNYQRINSIIVLNNQLEINLTNKQNQSKILNISSNQESKSPIFDIQTSLIQPNEQKNEKGLSQIDEFADEYQQETSNKTNCYDSNIRQEQNNNNINLDFSSIQLKPLITNKFHEDNQNQTEQDNQQLQQCQDNDNQISLCNNQDLNIKQNQQSNQSCQHDPSIKESNQELSKQNSQIKIIIDQNQDEEIDQEQLQQQIRIQNENIEKINIVDLDVNNKQNLESQPQIEMNPKIETPPKEINMQEQKQDEYTCQIQSKNVDINYQESSDIQLQIQNNDIGKTQIQVSDNHLIINQTMEIEKIQESQINQEQQIIKQYQNNQVSHPLENKLNNDQQNNINQQQSVEQRLIQKKKKKQKKSRIINKKKKKEKKNKVEQNEQKLNDEKKQSYQIIDCENKQILQIEEKPNNEQIIIEQEQKNPICNLLQSLIKGFYGENIKINWNKYTFLKKKIQKKRSILLKSKLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S0P6</accession>
    <name>A0A8S1S0P6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MYND-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0010569</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S0P6"/>
    <dbReference type="OrthoDB" id="283753at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="5">
        <location>
            <begin position="100"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8132354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="281" mass="32932" checksum="093E7A5E3111CDA8" modified="2022-10-12" version="1">MKKQGIMSKTDQYAEFTYRPNATSPPSMQEFYESVINGIIKRFQLGLYQGEYPYYNELQSCLHEFIRLMNFKSEILVEVFQSITNLNLSKETFPNVVVPCASCGMFIYIVQHCTICYSAFYCDKTCLKNGRQKHESECQQAKMTPSIIYPLQVEIHCGSSLGDDVKIYFQHERKLSLLYRGQKFLNSMYKILLKNRWLKNNGYNIIENETSIQLQSTKKIDMKPTEDQGDPNYSDEDLYLKMPCVQEIKVIFLNQGAILQDIIKETYNLQTNFIHIYYFPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S2C8</accession>
    <name>A0A8S1S2C8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">dCMP deaminase</fullName>
            <ecNumber evidence="4">3.5.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">dCMP deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0030254</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.12" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S2C8"/>
    <dbReference type="OrthoDB" id="6710946at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004132">
        <property type="term" value="F:dCMP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009165">
        <property type="term" value="P:nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01286">
        <property type="entry name" value="deoxycytidylate_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015517">
        <property type="entry name" value="dCMP_deaminase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035105">
        <property type="entry name" value="Deoxycytidylate_deaminase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11086:SF18">
        <property type="entry name" value="DEOXYCYTIDYLATE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11086">
        <property type="entry name" value="DEOXYCYTIDYLATE DEAMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="6">
        <location>
            <begin position="153"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8134336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="299" mass="35084" checksum="274C466B5FDC533F" modified="2022-10-12" version="1">MSIIGVLGGICSGKMLFCQLMKELYDYQIVQFDQEMDLNKTAMNIYNNWNTPTIITAITTKEQIRVLKKRNYFHLIFLDAPSVIRYRRYCEKHGQIKFEDFVKLDDKQKYDLDFLELAKNCKVYLKNNTNTIEEFTNYLKQQKTKIIRPFRPDWDVYFMKIAYTVKQRSNCMKKSVGAVLVNNNKRILSTSYNGTPKVMKNCNEGGCDVCKESKTLDLILYKCMCIHAEEGCILELGITQTRNTTLYTTLFPCVWCCKALLQAEVSRIVYCEQGNWDLSLQIIQNLIKYDQIDVEQYIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S2Z8</accession>
    <name>A0A8S1S2Z8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0030068</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8133972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S2Z8"/>
    <dbReference type="OrthoDB" id="299705at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="106"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8133972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="212" mass="25251" checksum="7622BED85542DA80" modified="2022-10-12" version="1">MSLNCPNKQFNSEYYTLLNGLEQSFFKEETDQKDLMQLILSIAHLVEYFDLKKDPLKDYFLEKMQFILSRPFTICNLKKKEDDTSNIEMKKSRFSHFSQNVQKLEMQSLLQSNSEENEERIKLLQEDYEKQLKDQTLLVQMDLNHQMGKLKNKLLHRKNKSLFSPSCRINNEEDQFKRQNRQNTCQSPNSQNSQMEQFCLDDACPNVTLQNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S6M9</accession>
    <name>A0A8S1S6M9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0030400</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S6M9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8134624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="136" mass="16165" checksum="5A1FC083227930FF" modified="2022-10-12" version="1">MMQSIQNQMNKIVLKIQKQYQQDLILLHQSEQKKVRIGIKKKRRQSQIKNFLPDTPPQFLQTKNLKTEDQAPITSILQKEEQFQHQKQHLFLMGALSDHQLSILLNLLITLNYYIQYHKKSIKSFNQPQQLVLSID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S8V2</accession>
    <name>A0A8S1S8V2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0050180</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S8V2"/>
    <dbReference type="OrthoDB" id="292172at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8136498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="210" mass="24364" checksum="BB19552E597CC43E" modified="2022-10-12" version="1">MNFILILFIASIKALPLYLAVFADDQQESKVYMRLKVLDAVKILMNRYPQDQDVQYMYYELINNKTYRSPPNLHITTFYIGDNKDAEQSEYYKNFTVNLPQEMKIYAVALLPKRVIACVVKRQDYTVPIENKFPHMTTLLGNWTAVDSNVLMASLFDDYGPLNNIYYSLFEQSEIKVYSTLINGKGEKNLPAYVVKMPISIDGQTQYGFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S913</accession>
    <name>A0A8S1S913_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0040464</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S913"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8135870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="137" mass="16353" checksum="F0BFC2C2FCABFE4E" modified="2022-10-12" version="1">MMDNILVMQYQYFQLKLIHHLYFRMQKLIIQKFNQAVNMQKQLFLLHKQNHHKIQTQIDNFQGKCSYMLTQKSTENKYPFFSVIINELLVYGYVLFRVIQLVEGELQSIKFAVVSLDGNKKPPECIQADESFLQEMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SAF9</accession>
    <name>A0A8S1SAF9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0050116</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAPRE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SAF9"/>
    <dbReference type="OrthoDB" id="2119228at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1430:FF:000011">
        <property type="entry name" value="Microtubule-binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000028">
        <property type="entry name" value="RP/EB family microtubule-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004953">
        <property type="entry name" value="EB1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027328">
        <property type="entry name" value="MAPRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10623">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN RP/EB FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03271">
        <property type="entry name" value="EB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51230">
        <property type="entry name" value="EB1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="11">
        <location>
            <begin position="4"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="EB1 C-terminal" evidence="12">
        <location>
            <begin position="166"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="251"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="264"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8136370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="275" mass="32098" checksum="CF1EA9902FFBDEED" modified="2022-10-12" version="1">MNQEISRAELLQWVNDLLKTTINKIEQLGTGAIYCQLIDAVHPGKIQLSKVNWRAKQEYEFVNNFKILQQAFTKLGLQKPIEIEKLTKCKYQDNLEFLQWMKKYVDTHYVPKDYDPLTKRGNQDFEDPEKQIRNQSKSKVLTKNNTKDQQIIQNLSKNSSFSTIQILNNDFSNKENRQASMTDLLLQIETLKCERDFYYVKLKDLDGLMEQHLQQGLTADQICKGIKEILYNTQDKSIVVQQNGDLEVTYSENNEDSKSEICKTEQNQQDDISIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SCP4</accession>
    <name>A0A8S1SCP4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0050208</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SCP4"/>
    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17953">
        <property type="entry name" value="DEADc_DDX46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22387">
        <property type="entry name" value="KH-I_DDX46_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000079">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="10">
        <location>
            <begin position="505"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="537"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="726"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="172"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="259"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="348"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="6">
        <location>
            <begin position="505"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="83"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="120"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="173"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="191"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="283"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="310"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8136554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1159" mass="135223" checksum="CAB8BEA02F1ED602" modified="2022-10-12" version="1">MGKDKKKSKNRSRSNKKKEKKEKKEKKSKAKKDSIRKESIKKKESIKKESQKKNKKQKESKKKDKKSKKKSQSPSPINAQKKQAQDEQKLKDDEQKRLSRLMKAKLIKFQNSDDDDDDDKKEFVYKDPEVIEPILPPDEQLIYDWSQRNQEKQIQDALKKCRLVGEKLFYDSDGIEEDDNPEEQFQDENGNDNRQDVKMQEEDEHEVQKPVEKVDKQQQERFLIDKKTLNNDSDSDSEDENASKVKFFTKNLQSISKLKELQQQQTQQQFSRPQIKRKWREEQYDDQEEDPLEAFMRQVETEATQQADEDERRRKEEEEKEKKRKQAIEEELRKLNAQKVITYEDIIQNSINQNNEKKNTNGMDIEQVQEVQNQQKEELDQQVNEEDEEDNKFYEQFMKELKKKELEEKMRQREDEDEAEDVDLEQQQMLALLEEEPDIFDKQKKLEKKELKPVDHSTIDYQPFRKDFYREVSELVQMTTEEAKKLRQQLGDIKVRGKDVPKPIQNWYQCGLNDRILNVLIEKKKFLNPFPIQAQAIPCIMSGRDFIGIAETGSGKTLAYLLPLLRHVLDQPALKDGDGPIAIIMAPTRELAHQIYVNCRWFTSILNLNVVCCVGGAGIAGQLSDLKRGTEIVVCTPGRMIDVLTTSNGKITNLRRVTYVVIDEADRMFDLGFEPQICKIIQNIRPDRQLVMFSATFPKNVEQLAKRVLRKPIECIVGGRGQAGGNIEQIIEFMDESDKLYKLLLLFQEWYTKGSILIFVEKQTEADDLFKELLKYGYKSFVLHGGMDPQDREFTIHDFKKGIRTIMVATSVLARGLDIKHICLVINFSCPNHMEDYIHRIGRTGRAGQKGTAITFFTPQDEHLANDLVYLLEKSEQQLPEKLKEYQKSFMEKVKAGEAKIYRNKNRAGGGFTFGPEEEQKFQDFRTQMRKKFGLEGLMMDEQSSDDEKVLEEIAKGKLSEEERLKKQEERDRVERERIMQLIKDPALKSQILSEATKAANLCINSGGSREQVANAAMDAIKRVLKQHSQVNKSIEGGIEEAMQIINEFEERERNNHDFVSYDFEINDYPTQARLKILSKEFLNMIHELTNCQISQRGSLVEPGKKPLPGQKKLFLRIEGENEYFVMSAYKEIKRMAQDLTLNQIQDNYAGIQTNSKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SGQ6</accession>
    <name>A0A8S1SGQ6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphodiesterase</fullName>
            <ecNumber evidence="1">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0070475</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="1">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8139108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SGQ6"/>
    <dbReference type="OrthoDB" id="286557at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="2">
        <location>
            <begin position="571"/>
            <end position="895"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8139108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="895" mass="105224" checksum="FB7AFAAFD5984136" modified="2022-10-12" version="1">MRAIQELDTLLRETETGKIKLSLTLKDLAQLHHLQSRLQDCKFLNSIYSEESRIKQYLSKAVTVNLQIQDELCSIIESKMESITSNFNYLDKLILYILNQTMNKTTMKNVLRQISNIILGSNNFTLLRFQEKSVESVSTDEFIEQQLTQEQKLQLYQQFRGDQLEILTFKPTGQLQQIFKSSSLQVIRVPQKLNAQTHSSLIIYEAQQFAILPLSKPHQIKLICDQLITIGYDYLNENYHKDDLINSVRAIGQFSIKNAPILFQECIRNEIERKLNTKCEIIQSTKNSKDTSFAILENQTKCFIPVMSNHAVQFYLHMENVSLDIITFADFMSQLWQHFKHCLRELKDKEFLYQIIEVSTPTKLVIASDLHLNIIYQSGSVPSSWNFLDLSNQQIDCYQNLTEIKMEEIYKTMLLSSLDEVQQSKKGAIDAGQIIYHDKYVDIAIYVKKDEKNEINQFFIVFDQPKKRFESQRHSKSEIKVSETLASLKQEKIEKACLVLQESLNLNLQTEEIIDMLKEHALMTIIEQKEQITEDIRQTYVLEYYRNSEQVQTYRRTISESDTPFATKTLIQEQQLTNAKKMELFQLRPDDLENVNNWCFDITIQKDQIKHYTWALFHLLNYFDKYQMYKETFFQFLVAIEERYNSRRNPFHNFEHGFTVAHACYYMIKNKLLDEYLDQTEQFAAILSSLCHDIDHSGRTNGFEVARMSKLAVRYNDESVLENHHAAMTFKIMQREKYNILSNLSQEQFQKVRRFMVSNILATDMKKHFDLVSSLEIKYKNGEMNIINMETKRILSGLIVHTCDLTQPTKRFEITKKWSIRIQNEFDNQVEEERLLGLPVTQHLVCQNLPKQELSFIRNIIQPLYVLTSSILNNGLSVALKCLEENEKEWTKLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SGT9</accession>
    <name>A0A8S1SGT9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0080243</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAD-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SGT9"/>
    <dbReference type="OrthoDB" id="295956at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004174">
        <property type="term" value="F:electron-transferring-flavoprotein dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735:SF3">
        <property type="entry name" value="FERROPTOSIS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="6">
        <location>
            <begin position="7"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8139685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="393" mass="45001" checksum="A1E2F4D3896908CC" modified="2022-10-12" version="1">MRNSKKTLLIVGGGFAGMTIITQTYKQFNIVLLDQKSYFEFVPSVFNAFIHPESIFDLTLQFKQSKFGVLFIQGKLTHIEDNIAYYEGGQIEFDYCAITIGSNYTNPIKSTIPKLSDRFIELKKTQLKIIESQTILIIGGGTVGVELACEIKASYKQKTVALITKGKILSTMPKSASEYAKKRMLNLGIEIQENYKGPSLDSNFDLVFNCKGNNYESVRLNDNYEMFDPKKQILVDDYLKIRTNQNIYCAGDICISSQNETKTAFAAEMQGEIVAYNLKHPNKQIKSYWIPNTYIISLGGWKAVFVFETFTFGGFIPYLMKLFIEVVVVNDFRGIIGFNTIHQIMNYIVYVMLYIYMIMQLLFAIAPLGSKIKQDQRVELKRIQQEIEEFKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SIY3</accession>
    <name>A0A8S1SIY3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0080048</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SIY3"/>
    <dbReference type="OrthoDB" id="305830at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8139337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="27126" checksum="CCBB271506D56044" modified="2022-10-12" version="1">MQDLIQNNLRQDVQSIDNSNMNDQFAKKMQALLEYGLYDQAFERVMKNKQQIQESFQLLIELARLFNTFKQHKLALEIIKLSNVFDQQLDMHQDQQIIIFKQYYLETLIELVDIQEALKETKNIYNEQVRKIIQDELHLIESQKRVQIHQHNHHNNQINEHQHQQLNIEIPKEKQQPIYRLIGRKTFILLFILFLMIYYSLKKKSDIKQVLEKAKIIFRQLLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SKJ1</accession>
    <name>A0A8S1SKJ1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0090054</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SKJ1"/>
    <dbReference type="OrthoDB" id="432483at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="322"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="370"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="402"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="537"/>
            <end position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8140398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="892" mass="105869" checksum="E6BBECAD90BC7286" modified="2022-10-12" version="1">MNQTNLTDNSHISHCQLMIQEESQSGCNNSILYHSIHGQNQAVQSMKLEECQRIEDIPIQNEKPRQFQVQKKQFSQLMVHFNIHHFKRKMLSYIHPNKELSKNQFFMISDKSSSFKSYPKKQQQKTSIIQQNRAVVGFRSIIKDFKNTLIFQIKKKFQQLIEIIPILDPYSTKKLIWDGIITIMRIYLLFWIPILIAFKNGTLEDLNAYTLNVCSSAFLIDLAMQTFTIRFDKGFPVKDRYQLIQYQINFWTAIELFSFIISLFFSIQFHQNNFSMSVVEDDGWTKILLLLLIVQTKNILQFIENLQCVIKPSKSTNSLIELVKLICLILLIQHIFSCMWVIIGTYEHLTDQSSWLDKAKLDINQPWHDIYLEAMYFISVTTFTVGYGDITPQNTSEKCFTIIYMFFCTLQLSYSVNTIGSILIQLKENNEEIKQKMTAVTEHMRNRQMSRGLQFKVREYLNYYWQQENVQKKNETAEIIKLLPEELQKSIQREGSSNLINKCQFFKEKFTPSFLNQLVEIAYVQNFQPGIIINDLQNIYIIEYGCVEIIQNQTVVGQLNQFQQFGLKRVSLDLINETKYRTKSFTNLLIIPYTSLVQLVAQNQNELEIVSQANLSIINECMICKGYHATELCSQVHFIPDNEKVIKKYLYNSTQNRGRHKRKPKDQFQEWKNELKFFQDSAEQFQQDCNNEIELLFPNNEKQQQINVCTPRSLFEITDEKPKSMVLNMNIGQRGDFENKEISKNDRKQEIKCEINELNLYYQFEAISEKYKQIQFYNEKVQKDIFILYKRLEFLLGLGECQIDRYKSYKHYYKNWNIDRIIAEHNFALNYMQNRKNEKINLEQISKYLLFPKMYLQKYHSEKPSEILQQPKVNKNIRTSKKKKRIAIHPDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SNR5</accession>
    <name>A0A8S1SNR5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0080428</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8140052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SNR5"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000099">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF803">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="10">
        <location>
            <begin position="86"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8140052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="478" mass="54025" checksum="386D59A57374FF13" modified="2022-10-12" version="1">MNYKSRDAPSFLNKYLQRDINPKRPFESQNKSYQKYDVSPLIKQPVRKTLELNFSSLEEHSMKKQAILPNYEPTKCSSGRNGIIRAYAANTNQGIVRDYNEDRVSIILNIVKPQNRATENWPKCSFFGVYDGHGGAACADFLRDSLHSFVIKEPEFPWNPVGAIRKGFEAAENHFLAYALDQYSKGIQERSGSCAIVCLIVGDVCYVANVGDSRAVLSSQKGKRVTNLSIDHKPETEAERIQRGGGKIYQTQGINEEGTQVTGPVRVMPGRLSVSRTFGDIEAKFEQFGGNSKVVISEPDVKIFKINQDHDFIVLGCDGIFDKMSSGDVINIIWQDIQNNNKSNLHSVLSTAVDSVLKEAIYKKSSDNVTLLIVAFQINQQKEEQKEIKYTYSNSVERIEETYHINSRPRISQQITKRNDENFSQFSSLNYCNQAASIHNSNKSALVQNFQNRLLKQQQSKKSCLDEVTNKIKTSYII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1STY1</accession>
    <name>A0A8S1STY1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0110366</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1STY1"/>
    <dbReference type="OrthoDB" id="301936at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="515"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="564"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="519"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="532"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="571"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8142846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="731" mass="87086" checksum="141F3243804C569A" modified="2022-10-12" version="1">MYQTQTQSQKQIEEMIAYSQSNLSIILNMEQFMKYFRANDPFAIDFLQRNVKQMIDILFIDYDKVQTDQLAIFSDLINKIFEIIDLKLEPIMKQIEIYWEVIFDLRWRALTPDTQWGIAHKLFQLIKDRDKRFVLDQFQRPNFLVSFLPLIHINSVAQILIIFYEFGFHQQQLDFLKVGFDRFQDFDPCSAINFTFIIHEIMIRILNDNQIEYVIKGEIQRKMFAIISRDDINAITLKNAAHILSLVSNYYSMDMQNIQLDEPETQEITQNFRHTEFFEYFDVKIIAQILQQSFQNKTKVGLLIIKLVEVIDNLVRITDIQLWEKVDKANIMELIFNLVHKFNKQDIFISYVDNMISFIFERALNDYHPYWASKLILKNKIHEKHHRLIDKQIYQFESKLSLKLIQDGEFLPYYDYLKQIEEELMISHNWRTLKLHQMKQEEIHKNKLGEDPSSPQIEEKLIVSALDVEDTKYQEQDGIDESANNIFINCQSNSPSKQNYQEITKNIFETIPITRADSESNSESSYNESEQSVEKKFVQDKDFDRPIDPLSQLKEVLSELGLKSNQQNKLSHSDQLDREKVYKNRNLSNSLNHEEQKNKLMTASLKDDYDVPSPILQRRSSLQPVSNVKKLNKNFEEFHIDFLNNRKILKKDDNSILKVDEIDFEKVPSKQIIQVVFQKQNQEILLKTKLVVEPKEEETNLFPKFEKVEDIKFRQLQQKDLHFLEFLKNQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T129</accession>
    <name>A0A8S1T129_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0140122</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T129"/>
    <dbReference type="OrthoDB" id="10559465at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="108"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="128"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8145197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="551" mass="68370" checksum="96CFFBEEBA61C83A" modified="2022-10-12" version="1">MDQNDDDEKRKRIEEKKKIFLEKKKAKELEQKKQKEQEEQQQQMKKQEQSLENNVKKQTDNQSILDENLQQQLNEQQKQKEQQNKSETSKKQVEQSTQIMSLQKKENQQQQQNNQKSQKYDESNSKVIQIINKQNNNQQKTFTSDQKAKNKLIEDNNRNQKEDIQKKNDEPKLDQIRQDKDQQIQEDNKKQLLVINKKQDNDKQIKDQKQIKENEDKQKLKYEAEKLEKDRIDREKKEKERQEREKQDKIERENKQKQEREKQEKEKQERVRQEKEKQEREKQEREKQERVKQEREKQEREKQERDKQEREKQERVRQEREKQERDKQERVRQERVKQEKEKQEKEKQERDKQERDKQEREHQERINREKKQQNELQEKEKSQINCKIQIENINSLNENYQQIQNNKQNEKEQNQQRIDIKKQLKDQQQNNSSCEEGEIIQHSVNQNVQHFENIQDQNNIVISQQFVEQAHIRNENGIQRIKKRHCKIILQDLREKIYKYNQKDNKKKQKRLKIYKKAIYQFIQNLPEINEDQENKTQRFLDVFNYFDNII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T2L7</accession>
    <name>A0A8S1T2L7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0150088</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8145928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T2L7"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000085">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="220"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="73"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8145928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="519" mass="58504" checksum="02C413F64EDAC38B" modified="2022-10-12" version="1">MNPVKVFPLKSNEQQNTQSTSSSPQLKKKQTPKLIEKPFLLNMSPILNGQSPIKGKFNVKRLNVNPKQEDKFQFTQQQRPSQSTSSSPIKRVINGATKIESQSPMKLPNLRRADTQLNNNIQIKDETIQEMKSSKRKDTTTDGTSTQQIRGSIIKLISESTPDTRPSVSRAKLGSQTIEQDIKTKVSNQIVMLHGRSTSQATLVNTFILDNEMQDYVNLFYSKSQAGQNGQGQTKTNQDSIIVTNNLGGIKNRYIFSVCDGHGVYGHYVSQLVKKLLPNIIDQQIKSNIGMQEKDIGENHYNSITKAMTQSFLKMQKDLSNCGIDITFSGTTCSLVLISGPHLWCANIGDSRSILIQQQNNQKWKTIELSNDHKPDLPNEYKRIISSKGRVEPFISENGEMIGPPRVWLLHDQIPGLAMSRSFGDYVASTVGVSCEPEIIHYRINTNCAFLVVASDGVWEFFSNEEIQKIVVQYWQPNMTAKKLNEICDHIVKLSTQRWHQEDEVVDDISIVIAYLQKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T2R0</accession>
    <name>A0A8S1T2R0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small GTPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0150017</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8145816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T2R0"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8145816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="183" mass="21315" checksum="B79227300A88EC16" modified="2022-10-12" version="1">MNSFKICLVGESASGKSSILSILQNKPFNQNISQTIGCDYWRYTQENKSFEIYDTSGLDKFKYTTINSMKGSNLIIICFDCSSNKVDQHVEKWLKIVRTYKSVTPIIFIGNKIDKPIKQESKYINRLNQYYPFYFTSSKDSQKVKGVFNEIVNHIIQSNLIVERVQIRPRQDDQIKSSWCCFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T3R9</accession>
    <name>A0A8S1T3R9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0150250</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T3R9"/>
    <dbReference type="OrthoDB" id="291705at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008506">
        <property type="term" value="F:sucrose:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19432:SF35">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 45 MEMBER 3 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19432">
        <property type="entry name" value="SUGAR TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="325"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="367"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="401"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="469"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="499"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8146249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="520" mass="59597" checksum="E736CCDAB52493DD" modified="2022-10-12" version="1">MNSLKNLNTNEQVFLQKIQIYYLSFLFYKQYMSSLIMYEDEAIDHYNEYLMSKRKEVTEIKNYKFNGKFNSKPTKAFKLKNKKIHYLTQSQILALVVLTFAVEGFAIEMVFISPLFLKLDTPELLDSSIWLFPPVINLILYPFIRYYSESKSHQLKNAMIYFIIVSIMGIGLLADTKSLYVDSINKKTSVLIISIVSFTVMDVGLEMLGFACSALLEDYVNPKQQQKVQQYRTFVDSFGKFTGFLVSSVLAFNFIYLNFDNFIENLSFAYLVGLIMILIGFGMVLISFPRKGLDLVQSNHKMRFSINSFLPGITFLFQLPQNMKIFLLSHFFTCGSQLFVSVYATLWSGITMLEEGPQQYNEAIRNIVFDIGISWGALALCMSAILHLLTTLFLKQLGNRYTSTIYMIVNALAGASLLYTWSVNEFYSIFITLPFWGVQTAVLSELPYKLANQLEDDQVRDTIKGLLPQMLNLTTFFSQAFMFFIIPLGFLLFSSVDDISVSMFMSGIFSLIGSFLVCFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T619</accession>
    <name>A0A8S1T619_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nop domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0150165</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP5/NOP56 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T619"/>
    <dbReference type="OrthoDB" id="6780543at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.90:FF:000005">
        <property type="entry name" value="Nucleolar protein 5, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.4070:FF:000001">
        <property type="entry name" value="Probable Nucleolar protein 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045056">
        <property type="entry name" value="Nop56/Nop58"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012974">
        <property type="entry name" value="NOP58/56_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012976">
        <property type="entry name" value="NOSIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894">
        <property type="entry name" value="NUCLEOLAR PROTEIN 5 NUCLEOLAR PROTEIN NOP5 NOP58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894:SF1">
        <property type="entry name" value="NUCLEOLAR PROTEIN 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08156">
        <property type="entry name" value="NOP5NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00931">
        <property type="entry name" value="NOSIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Nop" evidence="6">
        <location>
            <begin position="284"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="410"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8146082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="468" mass="52772" checksum="18D6E3252A567EC5" modified="2022-10-12" version="1">MLILIETPAGFALFQVANIKALNKIDNIYDYLQNEKQAKKLITPFAFQQFKDTQEALVATSKLINGKIPKKLSKFLEKNVISQEVQDQIAVQDKKLAKQLQEQLGLNCIQTPVTEQLFRGIRSQLTNLIEGLSESELKNMTLGLAHGLSRYKLKFSTEKVDTMIIQAIALLDDLDKEINNYMMRLREWFGWHFPELGKIITDNLIYAKVVKAIGMRIKTSSTDLSGILPENLEADVKQAAEVSFGTEITKEDEKFILCLADQVIELTEYRFQLSEYLKNRMQAIAPNLTTMVGELVGARLISHAGSLVNLAKYPASTVQILGAEKALFKAIRTKHNTPKYGLIFQASLVGSAPAKLKGKVSRTLAAKTALCIRYDALGEGQDAEFGITNKSFLEKRVHQLEEGVNYRDVKAPQRGKAKPIQSQTQYQEEADFQPQGSNWMQKFQKGEDKRQASQEIVQRTQQKKVKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T9L6</accession>
    <name>A0A8S1T9L6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0170200</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T9L6"/>
    <dbReference type="OrthoDB" id="305378at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="108"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8147904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="231" mass="27259" checksum="B4E01894A083854C" modified="2022-10-12" version="1">MQSTNNSQRSNYLQNSSSSVRRARIFDTSQSSERRNTPSIIPISNSQVNRSPQIQTEEFNVQGLQQSQVSEVQINDLNKVNQFQKLPDLSRCKVKFIKRENQYTQTEADDKDLIIRQQEELIKQLQRQIQQQSNTNMEFSFHKENMDQQLHSPKYNCVTNNQVNQSIGNNTLMTNSPYKKQQRQQQNQFYFTAIPQSNNKSYILKPHNTDKQVLEYTKQKSNKDYFFSQMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TCN0</accession>
    <name>A0A8S1TCN0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0220224</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8151991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TCN0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="5"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8151991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="195" mass="23561" checksum="9DF9B8A567C3D7C9" modified="2022-10-12" version="1">MNKEVQDLQEALEYQVNVILRLEDEQLLLQKENNLQKEKLKELNSMLQKYIGQQLFVLKNTPKQNYILNRILKFKHKRMKSFLIIHYVIPLSLQSINHPLFNNRINPILPKCQIKQNLQQNSLLKESLQKNQNFLEIIIKKIRIKTIKKKKKQSQKIQKNQIHNMKHQLKILLIQLNNYNNQKQLLILYHLQQRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TE54</accession>
    <name>A0A8S1TE54_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Calcium-dependent protein kinase 1</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="ORF">PPENT_87.1.T0190138</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cell membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Parasitophorous vacuole membrane</location>
            <topology evidence="24">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="28">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020002">
        <property type="term" value="C:host cell plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020005">
        <property type="term" value="C:symbiont-containing vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000398">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000557">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17" id="KW-0969">Cilium</keyword>
    <keyword evidence="15" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1032">Host cell membrane</keyword>
    <keyword evidence="16" id="KW-1043">Host membrane</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0519">Myristate</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0564">Palmitate</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="26">
        <location>
            <begin position="1"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="27">
        <location>
            <begin position="238"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="27">
        <location>
            <begin position="276"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="26">
        <location>
            <begin position="287"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="27">
        <location>
            <begin position="312"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="27">
        <location>
            <begin position="349"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="27">
        <location>
            <begin position="622"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="27">
        <location>
            <begin position="660"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="27">
        <location>
            <begin position="696"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="27">
        <location>
            <begin position="733"/>
            <end position="762"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00060437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00068067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="EMBL" id="CAD8149496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="762" mass="87615" checksum="7F8F390C6F6E195F" modified="2022-10-12" version="1">MKMHSHFLFLGSGTVGFKSANFCFIQIFLNQFASSQGPDLSGHSKKNQRILFXHNQKIVHRDLKPENLLFVSESLDADLKVIDFGTSRKFETGKRMTKRLGTPYYIAPEVLLENYNEKCDVWSCGIILYILLCGYPPFSGRSESEILKRVKAAQLKFDHEDWAHISQDAQNLIKNMLNPNPAKRLSAEEAYNDKWIQNNAPSNVINQKALQNLQQFHAKSKFKQAVLTFMATQIITQQEQDELNKTFKAIDKNGDGKLSRQELIDGYTQVTNNQELAIIQVDHIMELVDINRSGEVDFTEFLIAAMNQEKFLSVQKMEQAFKVIDLDGDNYISKAELQNVMGDIDDEIWIQILKECDNDNDGKISLEEFSSLLQSKYLDHPHIVKLYELFEDDKNYYLVTEYCSGGELFDRIKSMNFFSEKKAAELMRQILSAVWYCHNQKIVHRDLKPENLLFVSESLDADLKVIDFGTSRKFETGKRMTKRLGTPYYIAPEVLLENYNEKCDVWSCGIILYILLCGYPPFSGRSESEILKRVKAAQLKFDHEDWAHISQDAQNLIKNMLNPNPAKRLSAEEAYNDKWIQNNAPSNVINQKALQNLQQFHAKSKFKQAVLTFMATQIITQQEQDELNKTFKAIDKNGDGKLSRQELIDGYTQVTNNQELAIIQVDHIMELVDINRSGEVDFTEFLIAAMNQEKFLSVQKMEQAFKVIDLDGDNYISKAELQNVMGDIDDEIWIQILKECDNDNDGKISLEEFSSLLQSKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TFC4</accession>
    <name>A0A8S1TFC4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0220163</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8151905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TFC4"/>
    <dbReference type="OrthoDB" id="303625at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8151905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="461" mass="54501" checksum="DCD0E3D8E43DE53D" modified="2022-10-12" version="1">MDHIAITNILKSYGFKGAEPQYKTINYSIVPKHLSLEDHIEKPQLEILQQLNEEKKCRELVEIHGITLEKTKQFYADKIKEYNDIIDLKHIKNKRNQSQSVEKSNNQAFLRFAGDEGKITNFHISINSKNLIPLLNEINKRSSNEQTIQQSPLSCKRSNRYNQNNTLKTSNNYTNIKYNLQTIKSKQPQNIIQRLEQKLNNFDSLNQSQVSPMNQVNFNEDLSLDMIKKRKSFSIDLRFKRRNSEKKKTNTYVKQLKQEIPKIDLSQLISDSFRQNISSSCQYDDEQEDQQRESQFNYNNLELTQIQYPSPESKIEQFKSIIQDQTEKEQLPDIYKDIKINQNVDFEQRKKILKRRKQVVHDILNKQKPKFLHICGSKFPVINSEQNWNDYNNLRVRIIERELQQKMNQLRGPKDQLQDFENLSILSGPPINTNYITNNLKSDYQDVKVTKIMKQLTALKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TFV4</accession>
    <name>A0A8S1TFV4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0200399</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TFV4"/>
    <dbReference type="OrthoDB" id="298557at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8150667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="446" mass="53416" checksum="8B2C494DE26BFAA3" modified="2022-10-12" version="1">MKIFWLRPCAGDSLQSTMVYQKQKQKTIKQISTPQRESTKFPSHLSFEKAKTIIENVNNKILSDQVRTLLNTPVKNWMQLHNNLNNLEKCYDFFNIKVNSLFQQLVDQIFKKQNENYQIKTLIITLDYYMNIQTFLLFLYWRLLRNSEDLVLIEKTLYQIIKIKYICLNVDFLMEHFIHFLFDNRIKLRQVKPYEIIPNPLIGHEEMDLFQIDPTVLSNAITEINEEFYSFLQFDNLLTNLDNGKQSQFLSEYFDRINSFSLYIQASILQKKDRKKAIDYFYEVCLELCKNEDGEGVFLLFTLSIERLDQDLLHTIQYISPIQQQILIDMTKTFVCNKHIYTPTNTKKLYPIPSFYHFWTHLKKLEQAAQQSKNLKYIDQIRIMLLDLMFIRQSNYSRLLYRKIQSDPLLKYFLLKGHQTELSQILEIPIGNWNQMQLKLMKMASI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TJR2</accession>
    <name>A0A8S1TJR2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0210327</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TJR2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8151386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="174" mass="21134" checksum="D1DBBC686CD3B37C" modified="2022-10-12" version="1">MLEKMLKFQVVTKEEKMNSLQPLLPKIQINISDKQHNYTETIDTIGNRQCGSYRNYSQKRNQIKSFRRSKVVGYSQMIDNYKNASRQSQENPFSEELLIKQLKNNRNQEQFTERQYTVMMDYQQNKYHTIEATIKNIRYKSQPKKLECNEMDMKITPYIHQNKRFQCIDYYYII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TKG0</accession>
    <name>A0A8S1TKG0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C3HC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0230237</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TKG0"/>
    <dbReference type="OrthoDB" id="310062at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012935">
        <property type="entry name" value="NuBaID_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07967">
        <property type="entry name" value="zf-C3HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C3HC-type" evidence="5">
        <location>
            <begin position="7"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="645"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="230"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="421"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="489"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="651"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="661"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="686"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="712"/>
            <end position="724"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8152780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="724" mass="87304" checksum="1432000CA063C52B" modified="2022-10-12" version="1">MYKQYFDEYCERLKTYDFLNWPYRDSKLKPSKMASIGYICQEDCLQCYYCQSKLIIDDQMLPNDVILERAINKHDQYCQGMAIKFKITQEFLHSYGMAYYNECLASWKDPIEVKKTYLDKVPQNLQYLLQNPKQVLAIFGWIKQDQILLCKYCGRKCQILEEEFDVFSEHRYFCKWANDGKNIKYGYLIVLQQLRDQSTQNDQDANDRDIEQDQVRQTLVTQCENIISINLLTNKELQKAKAELQTFQDQLIKRKRFDIKESEREMEEFELEIQKRVKVQKEQIEERNKEKINMINKQDSEEEEELDMAVQEQQIQNTNNKMNELNNHNYQQDNQIVNELDNKIEQEDNQKQEFNKEQIENLENQNQIQEIKEQEKNENIQTNQIQEESIQQQTFVQNNESNLGLIQQPNQTEQLLNQQQGDRLEQDIEIHQEQRKNENIQSSEEIKELQEKKENVCINNILDQQHNKNSDFEQHNNQQINQNDNQIDEKQENQQMNICQDQIEQNEKQLQIEKVQQSQNQSQEIEIEQEKKESNNIVDLQEVNQKIQKPLQDEKIKEFEIKNNVIQDDQMISEQQQVDIIDKEKKTNQQDDKNNIEFQVEEQSEKNLETLKNGLKLTGQNDDENLRNQEENKIMTIINLEDQLKSRQQEDQDIEQEQEQEQQQQEQQQQEQEQQQQEQEQEQQEQEQQQQQQQQQQQQQEQQDNQNQIDIQKNENVDQDQSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TSR2</accession>
    <name>A0A8S1TSR2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0290212</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TSR2"/>
    <dbReference type="OrthoDB" id="425490at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8156861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="310" mass="36861" checksum="1C7B23C43C4E5AA7" modified="2022-10-12" version="1">MSEKKIRKKYHDKIDFKLDLPDTNNKKEKARQSAKNTRQRKKAYIESLEQKCEVTQLTAQLNQNKKVESKPSLMQLMEQFLIDRKQQFDMLEKAYQEGNEANIKVITNSLRFKYGISGQGRLMAINKVCELLIDLLLPMHMRYLFWVASENVDIFKRERQSSEFYSFDMSDSQINCLNSFGLDDDQILKIQNMQLIIQEQRSNYDNLIIEIYKIQQSIVKESQKCQTIVDEIWDVLEPKQAAGLLITLDKSRIEQFREKKVYPDCVILTLQKRAKEEIQRQQQFSYFEMKKEQKQEELGLNNYFLGDYNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TV10</accession>
    <name>A0A8S1TV10_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0260178</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8154859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TV10"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8154859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="262" mass="30479" checksum="E302B81392C2DBAF" modified="2022-10-12" version="1">MQEEQSKKVDPMDAFRKKEDYREACQKLDGLHSQNKENDETKYLMLFKKQETSKKDLCVIIRVYNGNMLWMQKIDRAALDEMIQKTDIKGTYSSFCELIRFAISENSYDFVQFESQLDIVLYFSLQRHVQLKGQIPLDQGLPITNDEDTRKIQFQFVCDLVDVLKATQLQFTKEIEVNTTRKKKNEQMIDVEQPPLQKPPPPPKKNLLAIGQEILTTPEVLLPRKGPYGIPFYGNQYVQFPSNADLINPNKKKRKPNGGKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U149</accession>
    <name>A0A8S1U149_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0310248</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U149"/>
    <dbReference type="OrthoDB" id="304931at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="260"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="298"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="382"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8158258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="646" mass="77258" checksum="8371AB605CD42026" modified="2022-10-12" version="1">MQQENEPTSISISRRAAYNYIKPILHEDSLVQTPTTSFRSCFKQITQKRSIFKSQHLTNEIIVNQFPLQESFDEIGKYQSQYFLTVNQIASILNSTFHTIICIYVLILQFFHQTEQLILLRIIGMILLFFHLILCLLLKQNILDASQLATLIFYIVTFIFENQILIIYLFISIFHIQTLLNVIKVSIIIISKQYGFTQFLLLEIIILIYTVHIYANFLIIFMQESIDSYSFWFIYLKSINYSIKILLFDFQIDDESTKNQLANTFFIIFNINYYFLLLKRYEKHKKSYFLYDHFQMFINNRCIQINISLLEMFNFVWILLKLQFQDQFLKKLENHKIQIYVDQLKKCQIIEKIFSEEFIRNLAQEAKIINVFKNEIIPNPGLYIVIQGGMKVQIQAEYEIKQILKQGDYFGLIEMILNKSQNLFLKSIKEESLLIYISGEDFHYKIKEFQEDYEIMRYIHDQLLFTQSTTKIKQKCYFCQEYHIHSQCKLINFKPEFDLEKLKLDQLNDRRYFQRVNTKRTFQIRNISESSKDSNQFEESFEIDPISSDILNDRQIGKNILGSLSQMIVCNSKISQEGIPQIHQTQSFKVQESQTQQNQLNTTQGWRIFDQENKNICIDNLKNFQFYDPVYNIETIIRKVNKMLEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UB32</accession>
    <name>A0A8S1UB32_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0370179</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8162058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UB32"/>
    <dbReference type="OrthoDB" id="306763at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050907">
        <property type="entry name" value="SRSF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23147">
        <property type="entry name" value="SERINE/ARGININE RICH SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="613"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="693"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="704"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="87"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="489"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="711"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="723"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="736"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="749"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="784"/>
            <end position="807"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8162058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="807" mass="94759" checksum="49F177C8FA5EA35D" modified="2022-10-12" version="1">MDQEINHFNLPDIRDKDDSDDQEDYNPFIYHHQQEGYHSFEKMFQNHFDIVNNRWITIPRTKIYLLRSAMHKLKKWLLYLRQEHHMIQSIVKQNDDLKTQLKKNQEQNSNHYLVEQQRTNQLYEEIRKVRQELKDKVIQMNDQIQQLKDTIAEKDKKIQRQELTIQRLNEVSNGYENQNKGLQQQINNLQKDFTYFNNIKRQGFIDEKALIGGNMTTFELVLASKVKSKILQFLSLKDYQNLMICNRKLYLSLISHNTIIPHAVNSLSYKFNHQYKLLQSVNEEFKSVIKQSDDPKLREIMIKQFELKVNFSEYIIPTLRECQSLIDGDNFIGLKNNQHNKDLMDLLEGYLTQQAQSYQAALQSLVQEKFPWNQFFPKPQATIQQVQLQRKQFPTSSQMFAQQIITRLYDLSTVCAQDSSIFGMFLSQLQRCLASLFVYGQHLTQEVNDLQSLNYYIMTKFFFLLKEKKQIEDQYEDFKTQLGLYSDAKRFLNEKVKDLEKIIQDKNEKISSLNQQIYMKESQLKQNQIMVQKNNESIRLYQDKLKILAREIMEVRRKYKNTCVEYNEIVSRENGKALKKLSIKIILKKVSFIQNKMSSSSSSRSQSPKKNKKRVYVTGYSLKEDQHDIKKLFKKFGKIEEFAWKGKYCFIEFKDSEDAEKAVKKMNKEEVKGSVLQVEMARGNKPSKNNGLCYSCGRSGHFAKNCRHRSSSSSSSSSSSESRRKHKKKHKKKKSSSSSDSSSSSSSSRKIKNKKKSTKRKSKSSSRGSSKDSQHSESSAGSIIDEKKEEKNPIEEENQGKEGDGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UBC4</accession>
    <name>A0A8S1UBC4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0370224</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8162148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UBC4"/>
    <dbReference type="OrthoDB" id="305634at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="535"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8162148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="679" mass="80490" checksum="9B6BA22703714504" modified="2022-10-12" version="1">MIKKTFSGISLRDKQKFTFKEQNGLLCYTFTQIVLIGKGEAKIYGLSNNKKIQVASLNEKQNQCKCKFIMDENNFTHLLCIGDESITINVLGYRLENQVQELQENNQHENLIIRDQSRVLKQKEVDSNEFELLSKKEKKEYKKIQNQKQLLQNLNIFFDMFKSIQKWRMRNQLKLYFLLLSIFSTLLIGIILVVTQNLVYKEVVLTTQQISTKLTDNEISNQFETQMHLFFEVNHKNYRALDKIAQLFTFVQLELSLITQKSDQCPVNQTQNYRKSNICYNIFNLKSGQELNQIQQKDLYYHLKHQSLLQELLFLFDPEQFIIGNWSIGYSKQSYFSAYQAIGYYSNFNIATRPFYTNHIKKANSSEYIFSDVFFNFENEYKVTISKNLTNDITDGIVATQFEFKIIKKFFQENFLILNKDGLILIGNVQMYFQPNRTNVFFFNESLTGFNENDWNSVKNYMDYNISEPNCTTLNSLYLCRYNKILLMDVMIFAKFLRFSNLILIILKNIEKDKDLEQQLIWIEDQKNLQITQLLTYQLIAALGTSLIAILIVRYICRYMTYLERLAHSHFQNKPVDFQVYSFLREIKKHSQSQSTNITLNLSDSYYKLISQLTARPFIKNDECKNFESFQFPCSKKQRNLPKWKSSILELYDKQGISKQKCKALILNLLRLSYQRHKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UCZ0</accession>
    <name>A0A8S1UCZ0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0360272</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UCZ0"/>
    <dbReference type="OrthoDB" id="9984778at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:001411">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46539">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ATL42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46539:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ATL42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="315"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8161609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="388" mass="45867" checksum="4DC355D1CDE70A27" modified="2022-10-12" version="1">MIILTFLNICSINCILYSFTAEHQYVLIEIFSEAWTFMKIEVAEEYVIQSSLQKNYTQYLQMYYQVEQGKQYDLDIFIQPEDTYKINQVEKRDCPNNCSQKGLCQKLECQCFEFIAGDRCQYDIEPIFDRSTYNIEMIPFQSKIIAIYLQQDDIVDDQQQIRNVRFTVSPSINATIYFLFPFEIPYKSLKNSKLVFDGIIDQEGQEIDFSNKFESYHLRSANYSFVFMLQNPYFLTLNFTIKSELIDNDNQFQDFILVIILSIVSFIILLVIIYIVKKRCQYMQEKKQKGNQSLNSQKGLLHLMGIVDPAKDQECIICLDLLDQKPCRITPCKHTLHEVCLNQWLQKQQNCPICRETFLDEEGYPKYVKTRNQTTTFLNHHISQYGIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UDY9</accession>
    <name>A0A8S1UDY9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0370119</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8161949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UDY9"/>
    <dbReference type="OrthoDB" id="668540at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010608">
        <property type="term" value="P:post-transcriptional regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF12">
        <property type="entry name" value="MATERNAL PROTEIN PUMILIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22493">
        <property type="entry name" value="PUF_NOP9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PUM-HD" evidence="4">
        <location>
            <begin position="55"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="115"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="151"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="188"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="224"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="19"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="25"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8161949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="391" mass="45403" checksum="F582F37B139ABDDE" modified="2022-10-12" version="1">MDPEDDQEIIDSIVNALESDEERSFSPSQTRSDSNRTYSPQIKRQQQTQYTIKKIQSPIIGFKLDCWAIQPFSLDDDQYLDGDLVLKCKDQTNSRKIQGLFEKGNDEQRDFIFQKLLPGIVTLANDIFGNYVVQRILEQGNQQQKELIFEHLSQQILVLSYNTYGCRVAQKLLEISYNTQKFEQIFKVISTQVRNLVIDTNGNHVIQKIAELLKSQKSDWLIEGVLGQIQKLSNDSHGCRLIQQILEISSISQLNDIYKELLSIQEELCLSQYGNYIIQILLLRGPIDVIYKIQNGIIKNLEKLSCDKFGSNVVDKSVNISIYMRKEILKVFIHNMNIFYKLSNNCYGNYVIQNFCKQLEYNQLLKLSYDSSQFNTQFGQHVYQTLQKLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UHS1</accession>
    <name>A0A8S1UHS1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Vacuolar protein sorting-associated protein 16 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0410183</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the VPS16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UHS1"/>
    <dbReference type="OrthoDB" id="1792at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016534">
        <property type="entry name" value="VPS16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006925">
        <property type="entry name" value="Vps16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006926">
        <property type="entry name" value="Vps16_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12811">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12811:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 16 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04840">
        <property type="entry name" value="Vps16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04841">
        <property type="entry name" value="Vps16_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007949">
        <property type="entry name" value="VPS16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vps16 N-terminal" evidence="5">
        <location>
            <begin position="3"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="Vps16 C-terminal" evidence="4">
        <location>
            <begin position="547"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="707"/>
            <end position="737"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR007949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8164731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="884" mass="103548" checksum="C1AF5F17D84F4750" modified="2022-10-12" version="1">MDPSAHWKELNRKYYKFHQFNPITGEQKSLDFFYQRIAVSKWGGPLAATKNYNQVILMRTDDLLKDSIVFFSNNGKIISKAQYKEIDRIALFDFLEDEQLLLLKANGSYYIVDPFKGTKKSYDLMDQFKQQQIHKGLIVNNGFVLMTTNYEFFYIPNAYEPQVHKMKPSNLTQEPEHWQVIPPQKTQSGKVELLITNPDGGIIHIIEDEKWKIYYNKDKIVNEIDKKLPDLRNIKMISLSSNYKNLALLQYNNARWVVTFISDFFDSTDCKQIPIDLKEEDIKKEESAEKDISKIERPRRMLWCGDDCVVLQLQQYIVLVSQDSYIKVKMSNSHFALKQEIDGVRILTQKKNEILRKLPEAYVNVFLPLSVKPGAQLHSAYESFEQKNPIEDDELRSKKAELGEAVNDCIKSGQFEINPEYQMKLLKAASYGKTFLGNQLIDPNLLNETCKYLRVSNALRRNGGIGSRVVTYEQVLQLIKNPDLFITLLLRYNLHYLAIEISRFLKFQIKQKSTIYTHWACCKVESQMDDDQLCQIIKEKIKEEKGVSFTQIAQKSIEIGKSQLALKLLDNEQSLSKRIPVLIWMANYQQGNNNSYYEKALIDAIASKDSNLIYLVIMKFLKTDMDETYKFGILSQNPVAQAHLIYYLRNFDDKYLQKYLQYLKKYDECGLLAINQAYQQNNLTEKIRFLEFAQKFFEEESKDSFYSKILQEQIKTLNDLKQEVEREKKEKSKVIMDERPLNSIMEAFLSKDNIQLAQEFAKTYKIPERRFNITRVKALINNKNWEELERFINEKNKKNVSIPYELVADMLIKADQEERGLQMIMKMPDPEESCYMLLKIGQQRHAVQVAINNKKSQLIQDIRGSINDNQARAQLEMYLSQNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UIJ8</accession>
    <name>A0A8S1UIJ8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0400264</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8164184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UIJ8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8164184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="518" mass="61028" checksum="F8788CFD57EEDB67" modified="2022-10-12" version="1">MSKKKRVSWFFESQQESLDAQNKSSLKINTVEEHKQDQRMPSQQIAQPMQVVPQIPQTMPYSQIDLESNRTRKVSFIPFIGRVAESTPPKLSTIFEVQPSSKKQEPQQNKIPVAQKIQLQQSIQKQPTIQTQLIQTPQQQPFRTPKAIQMEENSVKQSYRKNVKPIEIDIVKDLKQLNSLGEVNDATLKRVITKEEQEILLIQDLLSQKISKLQQKLIQLNEIEQRQLDSKMNNNSKLNQMQDKKNSVENQLYEPKQQEQKGSSLEIRATLIQCLGYKVTHINYDKKQYQIIFTYQQANVQYSFQNQKQIDGNLEIQDIFNEFYRELQENRLVLSGLKIDYKQKTEINKGEILQLSLKHQFSNSNKMANEIIFSAIHQMPILLFKVDELEQQLLFSNKIWGTLWKVDSEKGSIQILFSHRCRIDNKNLLSNTKKKVIRFEINLRTFEIQYQFYKYFDEVISPFNEKVSMRKKSTEEISFGERIESMVSDEFTEVIQDYLSRKPNLQQLVDWLNIIQLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UPC1</accession>
    <name>A0A8S1UPC1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0440042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UPC1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="223"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8166244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="299" mass="35655" checksum="A1274F7A200B3EDB" modified="2022-10-12" version="1">MILIQISKFHLSYNKQIYKYQKSFKSIKIKVLLTKQNFQLLIINIVIVVLMTMMVKYFLLKNFFNYIISHLNQTQKAGYSVFGIYNFKLKIKNKLESIDLFYDKSLDSNSALNKRNQLLITQFFNALKSFFVIALLHYQVNQSQKYFNIIYKSEDLSNIQLFISKQDFILLFIYSQSVLIVSDTNQDILLLKEIQLKLKSQNDFRCIFPKLYLQQNKNENKSVLYYIISYLIMIQIMPSQFEHEFIRSQCFMGDCRLQIISRRKQQLFNRQVVSLIIFLNNFNNKNAIYILIISSLSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UTQ5</accession>
    <name>A0A8S1UTQ5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0490147</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UTQ5"/>
    <dbReference type="OrthoDB" id="293914at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8168500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="289" mass="34198" checksum="A2372E1C4E0A0EE5" modified="2022-10-12" version="1">MQQLLYQHPLVIAIIIGLDSIKAFAIFKNDWIIADQKYLYQMSKNAQKLINKLEIQETQYIYTTSDKIIIQTKEGWIHIINEDFSKLNFFHLNIFSFCRLCIHQDLLFSINSNQLETRNINSLDIEFYEYQSEIFCTSIKVINSEFDQFIIIVLDNGIIDVLKNKTQIHKYQLSQNKQNPIFVYDCIPIIQNKELVIAISTNQQSIIILKNQVEQQTVNLSFSPTTIFSYFKTKLSPLIAFNKNRFALITNKEICKFNFTDITNYYFQKYCYLSTNEQIIIIDFDYLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UWM0</accession>
    <name>A0A8S1UWM0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0480112</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000048">
        <property type="protein sequence ID" value="CAD8167949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UWM0"/>
    <dbReference type="OrthoDB" id="286314at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8167949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="131" mass="15921" checksum="D02ABF67002A26DC" modified="2022-10-12" version="1">MDNEIQIENKVKRIKERYVYEAILLVEQWRSLYQQTYIENGRQLKLTLDKAAEIVGVPRKTLEDYYYQLKRAETLVDLQNFKNCKIGVIRRIVKESKKQSQKITHLIDTNEFFLQENQTNYYRKNSFEYDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UYH6</accession>
    <name>A0A8S1UYH6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="4">PPIase</shortName>
            <ecNumber evidence="4">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0470150</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="4">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDO010000047">
        <property type="protein sequence ID" value="CAD8167496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UYH6"/>
    <dbReference type="OrthoDB" id="271386at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01928">
        <property type="entry name" value="Cyclophilin_PPIL3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000093">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF2">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="5">
        <location>
            <begin position="6"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8167496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="164" mass="18562" checksum="904E93A374AA8686" modified="2022-10-12" version="1">MSVTLHTSHGDIKLELFCELTPKTCKNFLALCAKKFYDNTIFHRNIPGFIIQGGDPTGTGKGGECIYGKYFEDEIVPEIKHDRRGIVSMANAGKDTNQSQFFITYSKQNHLNGLYTAFGKVIYGWEALDLMEKETVDNNYKALNEIKIFKTTIHANPIADIEQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V0H3</accession>
    <name>A0A8S1V0H3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">H-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0520096</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000052">
        <property type="protein sequence ID" value="CAD8170013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V0H3"/>
    <dbReference type="OrthoDB" id="300512at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019019">
        <property type="entry name" value="H-type_lectin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09458">
        <property type="entry name" value="H_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035918906" description="H-type lectin domain-containing protein" evidence="1">
        <location>
            <begin position="17"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="H-type lectin" evidence="2">
        <location>
            <begin position="48"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8170013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="422" mass="48594" checksum="50B411AF4191BBDE" modified="2022-10-12" version="1">MEKFLFLLILFKVALSYIYYDTGLARDFDYTFDSSFSCKTGYSKTAIIQFSNQFEKIPQVFFTHEYFDQEMADLGFRLSITTITTTSFTTQISCNQQRVFTLKLRWFAIDDQRIEVISNFNMDTPDHKTFSIKNPNAQTGFIAITSFRYLGPIDFLLSISQITTNSVTVSITKVAGKFTNLKQIGYFVVVGVAEAFINLGLKTVTGGFSSGTLPIQSNRWFAIALQGLNYPNTRNMRIKAIFTDTSTTKSYTWGTWDEIETPNSHSQIWVAYQFTTSYQPLECFSIRTSRKQAIDLSILPTFFLQLVQPNQIYITNGNYEYIVDKSITPLKMNIQMKCENGKKVQADFNKCNSCSIQKTYSFTYNCFNQMNYIGFFPLFQQAFLQYNHLKINIQSSLLEVINVVYDQTITEKTIVKIQILNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V1D5</accession>
    <name>A0A8S1V1D5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0530127</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V1D5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="282"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="196"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8170574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="317" mass="37945" checksum="F74E5C98AA022481" modified="2022-10-12" version="1">MNQDSICQTHNQPTIGYYYDQEDMPHQFCSGCSLVNSPYRRYSYQDMINDKSKINDQKQRFMQILTELIEKQLEYIESINKKFKILIEEIKSECIVFGQKLEQMRETVQNEYPLRKGQITKLLDFQNYNINLVNNEIYTLCNQYFEDHYQNIYNTSQGNIQSLFTKMQNGIPQLLNTKLINNSDQKNLALYNLELNQFIDKQVQSLKNKIQEICQENAQLQDNTKRDQFYFKIESDKYYIKDISQLFIDIRNYIDISSQNIFYQSDIEKQLNDFMSAKKFEAQNNDQTENNNQTENNDSIINFSDDDDFYIQNEQIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V4U2</accession>
    <name>A0A8S1V4U2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Vesicle transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0570043</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="8">May be involved in fusion of retrograde transport vesicles derived from an endocytic compartment with the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the SFT2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V4U2"/>
    <dbReference type="OrthoDB" id="73614at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011691">
        <property type="entry name" value="Vesicle_transpt_SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137:SF6">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="30"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8172340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="158" mass="18002" checksum="2922CB46F13BEBA8" modified="2022-10-12" version="1">MMQIFDIGGPMKPKNENESLFPSLTYKERLMGFAFCSILGYFIQILSFGSFIGILGGSPNKFALTYSLGNILALFGTAFLIGFKKQFENMIDNERKMTSIIFASSLVMIFLSVYLFKSKFLVLIFLLTEFCSYTWYVASYIPFARDCIKGCLRNIIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V4V5</accession>
    <name>A0A8S1V4V5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0570180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V4V5"/>
    <dbReference type="OrthoDB" id="308652at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8172608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="457" mass="52799" checksum="E7DA1CF8DB41C783" modified="2022-10-12" version="1">MFCSKLNSKASDYLNILDVSSCSNKSRGSFKENQPDNRNFTPKNPNNRPQTARLDYSNPQKVKQFLNNLVQDEINKATTQLFGQNVLKKKENQISDSKGDGQFSSRTGNLVKQNTPKNNETFLKSTFIAPEQNRELDHRYFQNLYYQYNKKNDQSSSNSKGNLKSCQNTISQLNKQNNQKHSTPIRQNTINNENINTINMLLNSNQKNTSQHLNTKNQNSSSKKSYPQMINKSYLESFNSLKQKLDNTNQSKQSSSKKNIEKVINISPLNSPKLKGDISNNQFNDQTFQQTIIKLLFEKEQLWYDIFHSIENSTYSNLIQKSDQLQKLIILINEKQNSEYLFKIEPKILETLLYESQANFILISESQDQDGNEDHNVKNLIQYILNSNLYVFQVLQGTMTNNQIQNSIKQRIEIRSQNSRKQSTKGSQGLQKNNFIIKSILGLILKGLTKQFVPKFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V7K1</accession>
    <name>A0A8S1V7K1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">alkylglycerone-phosphate synthase</fullName>
            <ecNumber evidence="2">2.5.1.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0550229</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Glycerolipid metabolism; ether lipid biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.26" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V7K1"/>
    <dbReference type="OrthoDB" id="5332616at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008609">
        <property type="term" value="F:alkylglycerone-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025650">
        <property type="entry name" value="Alkyl-DHAP_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-bd_oxidored_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46568">
        <property type="entry name" value="ALKYLDIHYDROXYACETONEPHOSPHATE SYNTHASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46568:SF1">
        <property type="entry name" value="ALKYLDIHYDROXYACETONEPHOSPHATE SYNTHASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="7">
        <location>
            <begin position="141"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="7">
        <location>
            <begin position="496"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="517"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="242"/>
            <end position="248"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="255"/>
            <end position="258"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="307"/>
            <end position="313"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="454"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for enzyme activity" evidence="6">
        <location>
            <position position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR625650-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR625650-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR625650-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR625650-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8171819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="947" mass="108264" checksum="7377465652EA6152" modified="2022-10-12" version="1">MIRIQQLQSQIVEPAGLKGQYYEQRPQNKLEWFKNNGWGYKDTKFVLEQDGAVRLTGNKYRFSNQRMMKFKEWAEAKVGIDLSLNCEAQNEIPADTPIINEQFMNAIQGQFNEISFDNAQRILHSHGHTMQEIYELRHGKLARIVDCVVFINSHAQAELLVKLAVEYNVMLTVYGGGTNVTWALQCPKEERRMIVSVDTSRMNHVRWVDRKNMTALVETGVTGKDLEKELSRYGVVCGHEPDSVEFSTLGGWISTRASGMKKNRYGNIEDIILSVKVVTPTGTLSQSLDYPRVSSGPDLNQIVLGSEGTIGIITEAVIKIKAQPEVCKYESILFHNFALGTEFMYKLSRSKVWPASVRLVDNNQFQFGMALKTMPHSKREEFMDKVKKYFITQFMQYDPDQMCLVTVVFEGTKQEVEFQEKKVFELAKFYKGFRAGAENGERGYFLTYMIAYLRDFAMQFQFIAESFETAVGWKNVPAVCENIQRRIVEECSKRGVEKEPFVSFRISQVYDTGATIYVYFGFVYGGGTNVTWALQCPKEERRMIVSVDTSRMNHVRWVDRKNMTALVETGVTGKDLEKELSRYGVVCGHEPDSVEFSTLGGWISTRASGMKKNRYGNIEDIILSVKVVTPTGTLSQSLDYPRVSSGPDLNQIVLGSEGTIGIITEAVIKIKAQPEVCKYESILFHNFALGTEFMYKLSRSKVWPASVRLVDNNQFQFGMALKTMPHSKREEFMDKVKKYFITQFMQYDPDQMCLVTVVFEGTKQEVEFQEKKVFELAKFYKGFRAGAENGERGYFLTYMIAYLRDFAMQFQFIAESFETAVGWKNVPAVCENIQRRIVEECSKRGVEKEPFVSFRISQVYDTGATIYVYFGFGYKGIADPVKCYSEIEDAAREEIMKNGGSISHHHGVGKLRKQFMQRQIGDTGVEILKKIKQQIDPKNIFGNQNLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V7S6</accession>
    <name>A0A8S1V7S6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VPS9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0580015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000058">
        <property type="protein sequence ID" value="CAD8172731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V7S6"/>
    <dbReference type="OrthoDB" id="288948at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051984">
        <property type="entry name" value="Alsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003123">
        <property type="entry name" value="VPS9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46089">
        <property type="entry name" value="ALSIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46089:SF2">
        <property type="entry name" value="ALSIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02204">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00167">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51205">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VPS9" evidence="1">
        <location>
            <begin position="312"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8172731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="500" mass="59177" checksum="79A9AFBD108CDC05" modified="2022-10-12" version="1">MGNESAKSVTKDSSELLLSQHTKTIESSQVENSKIFVQENKLSYCIVCCNCFGLIKTQLVEIQLTWIDYTLRKLNRSEYKHQAWSDLIKQRLQNIDIKNFEDQQAIYLNKIRMIISSYETNSSMYQNKISNHYENIEQSKINIFEYYEEEANTIFEDRKKSCINDKQFFREVMGMINQQIKIVDHPCGLILQTFQNYIVNYTGPQVWFRDSFRRDKYKDQLIKSTELCDEIIEFLKFFVNCIDSYYMIDKFVQSTHLLNCFTSTNNIAFVINILFLDERIYIKILKSFSELYAFENYQYQLSLRILNQNTIADFDVHLQLQLNSNTQISFEFQQDYLKGNPSIQFSEYEDFNQPYQSAINTLKQLEQVQSPANQLKIISKTFKQINFCIHDYYEKHPELEALKSYGTDDIVPILTYVIVKCNIQNFGIILKIIEAFTPKQIMTGILGYYLVTIQGCYQRISQYEKFQQVRQTLQTTKPEGETVHQNQFQQKVLSSDQFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V8U6</accession>
    <name>A0A8S1V8U6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0600155</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000060">
        <property type="protein sequence ID" value="CAD8173958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V8U6"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8173958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="402" mass="47756" checksum="585F54B946742367" modified="2022-10-12" version="1">MSSQFQQGRSQSIRFLQTETIYEQPFFTPNVDILSISENLYLEKIKKQATSKDQSPQKRQESPKKPQMYQPKLVKHQEIHKDVKVLQVNYNEKAIQPIELISKPTTEHKQLIGRAKVLLLQQRSFNMKKYFKIATLAIIAAIRIKKSLNYSLWKKYQIQLKNITFFLKLELVQTERINAWCKAVCAKSLTSVNQQLHNFIDYQNDKEKVDFSMMKSINSLQFLLENLAFFTQPKYFCEELKYYLLSQVFLDHKSYFSKFVSNRTQYLHTKNRQIKQEELTMILVESIIVDQILVNIFDSNQKCLFLLKLVASLLQYYFIKKFNKLKQKKTIIQKFSAHQIIQLENKIKLNLILNPLNNEFLIIGLYRDEEIQNLLNYKNTTSQQMDLNFERFCCNLYTTILA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VJT3</accession>
    <name>A0A8S1VJT3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0660016</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000066">
        <property type="protein sequence ID" value="CAD8176843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VJT3"/>
    <dbReference type="OrthoDB" id="308066at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00195">
        <property type="entry name" value="UBCc_UEV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBC core" evidence="1">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8176843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="690" mass="82024" checksum="45D68B7957406BAA" modified="2022-10-12" version="1">MILYKLFENKQCISIHITENPTPGVEVYQDKEDGLKIHFILSGLASPLENQIIPFWIELPQNYPFSNPFIKIKKIIFHPLYFNDRAECCDCWYQSKWQPSHTIKSLINDLIDILLMPRVSHPNVATSILNHSKFEYFETALMYYKEEKQWEQSEVNFEEIVNLPCKLNHSRPIVEVIIDKNLKSDEMLICQQCQLSIGDIKRIDINQLQQNLVRNRRNRNVIIEDLENKFLTQIDNFQKCIEKIQDLDNQVFQELLYLKNDWIVSRLKFKQKLCSLTILEEIELIKYEPKEYKKQVKQEKQQIDGLFLQKIYSCIDKLVLQQNRQNLMEVIQNIFNKPQLKFKFQNIRYEFIRPNRMVNYTFSINFDNTLMVTSEIFSNSMVQIYQIVKGQNDGQYQLSYLRTQSFRYRIIYIKFLFKQRFTFVITTEYRGLFIMYPIQYNGEEAINWDFKQYIDDCEYVNCLEVRQSDDLIVTGHKNGNIMFFFSTTNQPIQIITESNTEVWGLSFNQSGNMLLSCQQNNEIFLMMEQKEGWSIKQKILMDGFGYRISFITDQIFAIQTRTPKPHNQIYIYTSDSKGQFIKSGSLFDNGGNQPCEIFFPMQYIPSKQLLISKNGYYLNVLKLAFSNQSRLDIERCRLEQSIYFGTNFFYGTVSNNGEFIISMSQVFPQKYNHSLEILIGTFKLKYQQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VL37</accession>
    <name>A0A8S1VL37_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA replication factor Cdt1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0670153</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000067">
        <property type="protein sequence ID" value="CAD8177361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VL37"/>
    <dbReference type="OrthoDB" id="296876at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032054">
        <property type="entry name" value="Cdt1_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16679">
        <property type="entry name" value="CDT1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA replication factor Cdt1 C-terminal" evidence="1">
        <location>
            <begin position="245"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8177361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="360" mass="42405" checksum="14D6917D2FF28F7B" modified="2022-10-12" version="1">MKNKNINAAPDLNQNLQKKVKLCEDSQCTTLFNENSNSVEQFPTLSKIASKYLSKPSLRERFQLINDTGNLQASKYYSLIQILSAIDSQLLFLKQRRESLFWPNIVKSCYDSTQLQVTTRQLLEILTVWQQSYIINWEKFEKQNGNYELLFKFPDKQLPNSQELNQRKELFKEKLESYLQLNGDFIEPYKLPQKPSLSNINLKQEKNISGKNDCVQFCQFINISRIFQDNDKSNESKPISNVSQKLIDKLKERKLLEKQKYEESQDDKQQSSKKQHVLIATMLHQYYQQRDVSNMFFSNVLKYIHEHNLNVLMSNDQIKIIIMNLIQSVENWLQLIDNPGGQILRLNKEIDLPNIINQLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VMK9</accession>
    <name>A0A8S1VMK9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0680102</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000068">
        <property type="protein sequence ID" value="CAD8177813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VMK9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8177813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="60" mass="6960" checksum="F0971AC548F30A57" modified="2022-10-12" version="1">MGCSIRKAKPRKIEDIDQIEQMIQSDCLGMDQKFVLIKNPIIIRRNCSKEKQKLSDSKPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VSB2</accession>
    <name>A0A8S1VSB2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0750087</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CFAP97 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000075">
        <property type="protein sequence ID" value="CAD8180878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VSB2"/>
    <dbReference type="OrthoDB" id="291385at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038791">
        <property type="entry name" value="Cfap97/Hemingway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029488">
        <property type="entry name" value="Hmw/CFAP97"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23035">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 97-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23035:SF2">
        <property type="entry name" value="KIAA1430 HOMOLOGUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13879">
        <property type="entry name" value="Hmw_CFAP97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="164"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="179"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8180878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="226" mass="26803" checksum="8221C29BD57325C0" modified="2022-10-12" version="1">MKGPKYVEQDIIKKVCQAEQFQQHKFKLINTRSDPYLSRRHVLQDNDKYQQIRANKFYTKLQVDLQKEQRLFKENAKILNRIVDIGNQKTYSSLPKRSLTRQSSANSIKSLNLSYRKKEALKIVGENEKLMQRLQRTPSTFRNKETFLKDYKKTVELKNRISKYSQQNQQKMGKIVQRLTKTTTNPKFTKSNQSKTSAPSYKNSALSQLRIEPDRQQKKLQFPRIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VUM7</accession>
    <name>A0A8S1VUM7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">60S ribosomal protein L11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0730035</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000073">
        <property type="protein sequence ID" value="CAD8179865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VUM7"/>
    <dbReference type="OrthoDB" id="1734943at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1440.10:FF:000004">
        <property type="entry name" value="60S ribosomal protein L11, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_uL5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_uL5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057266">
        <property type="entry name" value="Ribosomal_uL5_euk/arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031310">
        <property type="entry name" value="Ribosomal_uL5_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003258">
        <property type="entry name" value="PRK04219.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00281">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002161">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL5 N-terminal" evidence="11">
        <location>
            <begin position="35"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL5 C-terminal" evidence="12">
        <location>
            <begin position="92"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8179865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="200" mass="22566" checksum="120339C88BE0C627" modified="2022-10-12" version="1">MSQQQGKGGKAQQTKTDGKPAAPAKVVKPQETKENVMRKIRIEKLMVHICAGESGDKLTKAAKVLEDLTGQKPVFGKARYTVRSFGIRRNEKISVFCTIRGDAAKDILFRGLRVKEMELKKRNFSDSGNFGFGIQEHIDLGLKYDPYTGIFGMDFYVILSRPGLRVAQRKSRNARLGTAQRVSKKEAMEWFKQTFEGNVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VUY7</accession>
    <name>A0A8S1VUY7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0730130</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000073">
        <property type="protein sequence ID" value="CAD8180053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VUY7"/>
    <dbReference type="OrthoDB" id="296892at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="209"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="335"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="254"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="344"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8180053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="738" mass="86352" checksum="9DD52BC8D6BC3223" modified="2022-10-12" version="1">MNKAFQRKHSDNSILKGKTQHHTQQRSPLSTKENDSKFKYPEVLRRKESKPLQVQTNQSDMRSPSAQSTQSPINKSILITHRSQSMFQDAQYIDYIITNKEVNLFLKKHFLHEHMHTNSSRNTNTKLICDSWDKVPVTYFIKALFDEYPEIIEDHHLNEKLNIKLKTLIKDLISEDGQLLSLNKLQIHTRHLGLLGLLLTALRDIQDAPTVNHNTPHNQSISHEQDISNTQQQQQSKFHKMASEITQSAENRINELVNLKLQQFTIKQKELEKQIQQLKQKATENFDLFQHEAKNKQVITNLTQQLQQKDQIIADLEQKLIKKWMNSSIEEETHANKIAQPKASPIKIQSPSQSPFNDSNQYLQRMISNLCTVWDLGCVNEDYRFAQILTAQENNQMPYQHFQLIPIIKTIPGFNDFIFKSQNNNHIITNFIQNCMNNDNNIILDLYYEIITLLPNFKISDQDQYLINVMQLFLKLTVSILQIKNVFNINKLKEWGSIKRQLTEQQNQFVKNLINQIVQINQYSIKQIPQSKLWNQQQIYHDQYVSEYNNRLIENQVINNILKKQLNQIHNKQNTKLSLIIRIIQCFYYNDVEELIGLMQSIQGETDVDIYLSKLILDCDCINLILFPSLFNPKYVLKSITNLLQFKCPGQFYLLYTKQVCQIRNIDLLIELLTKMHNTMNSDVVIKLWDVLGAIAKSAALKECKEGLQFIIRLYSNSHQATILHNIQQVQKNLAIPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VVA0</accession>
    <name>A0A8S1VVA0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0770077</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000077">
        <property type="protein sequence ID" value="CAD8181798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VVA0"/>
    <dbReference type="OrthoDB" id="309946at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="961"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1085"/>
            <end position="1105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8181798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1224" mass="146790" checksum="C4AFF139A1D1EF6B" modified="2022-10-12" version="1">MHTLFKETKDIKQLELKVQVFKEFQKNNQIVNKVGDLIYFGHIYHHLANKCKNKYTEYGMRCFCTMKITYDSKLQKDIRKTIQAIRNHRSSFTKLMIKSLFEILIQNQPRNIDLRFIYAQFLYFKMKNRIQAYEQIQQIQKKQLNLHLQLRIALMISMIQDNVTDINSLAYHSVLDFEYVVEMEETVSQIYQVVLRVLNLNSLFWRKLNKNPQLEQALIDINKDISEEMTNCNKLWKNLMKHEVRVKQQDVITTYLNKRLIWKFVYFWYRLFILNKKIRLTQIECVSSATLHQEIINEDSDSDYEIKAYDQFSNSKPFNHMSLIIHSLLNGQIIKCSQSCKEVLEYENLKSIYQLIPEVFQRNHQNAIKQFLYQGKSKNLHKSRKIFVRHSQGYIIQARKYLKWQLDKQNQINFIAMIRPIIRLEQINFIILNGDWEVDAVTRPLFQILQQKTCLLLLCTGLFQFSKYGHYLDSDDITRFKLNSKSKSLNMTQSKYQNMNDSIHFNSLTNSSKINTMDKYHIESEDQSFAFFGEDNIEYYNTDNTFTNKRLFKIVDEQHVKLTLRLPKKLNQLNQEYEYLKTSTFQDVIQQTDLSTIYQNKNRLIFRNSENKIKINKLLLFRRLYDFNQRVMKQMFDNLRDLFEKYCSQNRLLDKIIKLDATIHFTKTISMDHYTIIKINKYEIFEYHQRQFRETHNILTESRIRQSKPSLVEMYMDPRIFGIQQQEVDSNRIDQNKSFLTEGQVFPEFVQYHPTEVVLKLENNIFFKQDNNKISKNIKEISFFIFFNRIMITTLYLLTTIAYFKGPSLTQHAQIYSEIQKLQLISKLSLCVIQTYEYTLDFYLFNLQNASDIDVEQYLLKQSDLLQQQMSFINQSLTSKDFIEIIFGYQMLENPINDLISQFMAILNQCNKSNWQFINSQFNFLDEFRLIIIPDIQVGLLNSYLQQYNNQMNRLYEYQQFAIFEIIITICLIIFFIIANISLIIKIINKNKVLLNIIQTIIKSLHLLSQQQITTSIQYITWLKLQLRFLIDLETMKFLNSKATQTSYCDQLTKYENQEQNSQINSKANLYIGSSQWIKIKITFFLYYFFFSLIISSFVFYYLLFLQAFQNGALMIFNENEFVKNRNHLYAMVSIKELYIYQYINNSNIAIEGIKNNVQDFIDQIEDEQDQIYETNIDQVFQMFYGNYCQILLVLIQIKWSIYQRIKSISFNIELNCIIIDKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VWN9</accession>
    <name>A0A8S1VWN9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA-directed RNA polymerase subunit</fullName>
            <ecNumber evidence="12">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPENT_87.1.T0740018</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="12">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="12"/>
    <dbReference type="EMBL" id="CAJJDO010000074">
        <property type="protein sequence ID" value="CAD8180262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="270392at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02733">
        <property type="entry name" value="RNAP_II_RPB1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000019">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.390:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.274.100:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007075">
        <property type="entry name" value="RNA_pol_Rpb1_6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF37">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04992">
        <property type="entry name" value="RNA_pol_Rpb1_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 12" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="14">
        <location>
            <begin position="228"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1594"/>
            <end position="1748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1649"/>
            <end position="1681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1682"/>
            <end position="1695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1696"/>
            <end position="1732"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8180262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1748" mass="200680" checksum="A19EC991B2E1A2F9" modified="2022-10-12" version="1">MRQSGADFTYSTAPIAKVRRVEFGLMSHELIKEWTGEIEIKELQTNELDGTPKQNGLNDLRMGVNTNSQKCKTCGETKYCQGHFGRIELNKPVYHVGFLQIVKKVLKCICHSCGKLRQPQSEDAWLSFQKAKEHKLNRKRLNEIVKLLGRIEKCETSKHKSEENQGSEEFCGEKIPTRIQAINGQIKIQYNTKEAAKELTAERCLEIFKKIRDEDAIILGFTKDSRPRDLIIKYLLVMPPQVRPAIEMNPARIAQDQYTQIYKSILQKNNEIANCSSDAERVRLAPELMREVAKIIDSEKAGKIKMKSTQPLKSIRARLKGKEGRFRQNLMGKRVDFCARSVISPDANLGMDELGVPQIVADQLTIPEEVTEYNLERVIQLAKTNKIKYVIVPITDPRSKQRKFQALYFDFTDTEEQIRQKINQGVIVERCLQDGDFVLFNRQPTLHRMSMMGHRVRILPYSTFRLNLSVCTPYNADFDGDEMNMHVPQSYETIAELKYLAHVPRQIVTPKSNQPVMGIVQDSLLGCCLFTQRDTFLTKDQVMHLMMWNEQFTGELPMPAILKPQELWSGKQIMSMIIPQSINTERGIREDDLRKPNWNADDKSLCIQRGNLVSGIFNKELVGQGAGSVVHLCWLDLGAEKTLEFMTSCQRIVNNWLIMHSFTVGCQDIAPHINLVAETEKRSKEQDDEYIKLLQIFLDAKKIQDNRYHQKGKRIMDSFEYSFNMKLNKVRDDINSKVTETIDQIRNCMYKMIWAKSKGEASNLAQITSLVGQQNLESKRIQFGFAYRTLPHFSKFDYGPEARGFVASNFFKGLKPTEFFFHTMGGRDGLIDTAVKTSRTGYIQRKLIKAVEDVFVRYDSSCRDSVGAVYQFHYGEDKMAAEFIEHQEIKCVNLSDVQLEEKYELIKEQWFGSEIRQKYQNILTEDVIKDIEEDFEGQALLKGEFDEIKKIRNELRRLFLIDSAPKEELFTHYYLVVNIERIISTIKINKKISDREKCRLNPIHVTQQVDELIQKVDKLLSYDLEERRDCINLFRSHLKVSLASKDLCCRHRLTPEAFQELISEIIYKLKKSMAHPGEAVGAIAAQSLGEPTTQMTLNTFHKSGVTGDKNVTLGVPRLQELLDASKKTKTPSLTIYFDPPLEYAELELKDDKDKKAYYKTEQLAHPHKQDDFTQSMLVQMQGRILGQTFGQYIIKSEIYYQTDPNNPLHITEDQEDNFTDGIFDTDEAKYKWILFLHMDHQKILSNLETWDKIRDSIPKILDETEKKFGISSQNTIVDPDSMNHKYIQIKYYHDYDDKKKFKPERGKGNFDDEIAFDEENKIKTYVDTPYTILKRLEETIRGYSLGGISKITRILHSQIEKQFYINNKTGGFLKQANIDGKGKWKIIEKYLETEGTNLKEILRLEHVDQKRTTTDDVYEISQVLGIEAARAALVGETRKIFNHYGIYINYRHLYLLYDWMTHRGRLTAVNRNGINRIPEVSVLRKSSFEETVEILYDAAVFSEVDHMRGLSENIIFGQLCPHGTGSFELMVNAKNVKEFKLKSSHADKFTQGGEYLAEQSPYDQNLQTPQIINTPLPGVVSSYINSPYSPFPTKSPGFATPVNREYTPDMSHRSPFYSSTPLIPNDPYQLSPVDSISGLQAEPRKSDPVTGSHSPVSPHYTSHTNSPSPSYRSSERATSGQRSSSPILNSSRSPNYTSSIYNSPLSPSNTGSGRGQTGSPQTSQNIYSTYSPVYQPGGNPPNHYEQEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VZA1</accession>
    <name>A0A8S1VZA1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0790059</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000079">
        <property type="protein sequence ID" value="CAD8182660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VZA1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8182660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="55" mass="6752" checksum="F78028E5800EFD31" modified="2022-10-12" version="1">MQLKIVIDKFTQIHFNTRYHQKSIKRLNPEFYIQSNQNFIMNLKQETFGLSNLEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VZZ8</accession>
    <name>A0A8S1VZZ8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tetrapyrrole methylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0780020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VZZ8"/>
    <dbReference type="OrthoDB" id="289942at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11648">
        <property type="entry name" value="RsmI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008189">
        <property type="entry name" value="rRNA_ssu_MeTfrase_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46111">
        <property type="entry name" value="RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46111:SF1">
        <property type="entry name" value="RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="1">
        <location>
            <begin position="210"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8182093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="489" mass="56692" checksum="945F002928C97FAC" modified="2022-10-12" version="1">MIRFATRLVRQFSSKIDEIIDIGKPSLSKGILTICPTPIGNLQDWTPRQDKALFEADVIACEDTRITGFLIKMIKNKKLSNEQLAIPENPKEYDLDDDDFSIQDVIFDSTKQLQLKLPELNQQEITAADFNFYKQKKIEEMKERVTQEVKKQKEYLSEQDPLNFMGKQDYEEEMNPSEFEVYGLTAPFMVYLKTKIAVAKKRKGRGILISCHKFNEEKRIDRIIAMLKMGLNITLVCDAGTPAISDPGYQLVNKCIERNVKIEVIPGPSAISVALSTCGFPTENFSFLGFLSKEQHDRDENLRFFIKQPRTLVLFESPSRVHQTLLTIEKIFGETQQLWIGFELTKKFEKKIRGKCREVYEMLTDPKIIRPSHLKGEVTIIIAPYTTTYNDELKAEQFSTGNKQNEFYEEEEENIENLIKNVEALQVAQAFGEKFHGTDREFNEVLQKALRISKTKATKLIVEVRHMQRIEQNLKKIKERVGEQEEYFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W3W3</accession>
    <name>A0A8S1W3W3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0820159</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000082">
        <property type="protein sequence ID" value="CAD8184140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W3W3"/>
    <dbReference type="OrthoDB" id="1711136at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047126">
        <property type="entry name" value="RNF141-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109">
        <property type="entry name" value="RING FINGER PROTEIN 141-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109:SF5">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="370"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8184140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="434" mass="50980" checksum="C4408D986371C801" modified="2022-10-12" version="1">MNRQQEQINSQETEKEFSNEVIRICIFNCACQFYMLIFSCKLIWLFDSKYIIIFAWFMDLITFVIVMLDRNIGKVLSVVHFLEKVLTISFKITIVIHFEVKEIKLYYIPAVQLFITIGLFLFQVLHHDQSKRKHVIIAHSYSVLYVIQLLLLTLKWNNLFGYTYYQTFIIAWTALGINTFLIILLLITMVENCFLKHLPQINGTIIFWFIFYIFGLTIIPFFLLKTICDFYEGDTIYSNIQLASIAVLVCILIYLFLFIFFTLKIKKQLIVVLQLDHRQAITPKRPLQETTQKKWRKLKIPIMFIRISASYYKMLTRQSTIDTNTRNTSSIPICSDVLRPDSLNFRQSPCQTMMKQMKSIPKDQDSGDFCLICFENEPDVVLHPCNHGGICNNCSENMIKTTKQCFLCRSEIRYALKINQKDGEMLEATDVQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W455</accession>
    <name>A0A8S1W455_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0840200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8185158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W455"/>
    <dbReference type="OrthoDB" id="298306at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8185158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="123" mass="14448" checksum="AE880911B2D630DC" modified="2022-10-12" version="1">MYQRTILEDNENIQEDCKPKRYAASLEGQNFKKKQTNQASFQEWVGLIGIIVVTYLLIILVWALCFIGIDSNFKVTVGIFMVLFVLFVIGILIYWYYGLKSREELIRSMMLSKWQDSSYEINN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W5M8</accession>
    <name>A0A8S1W5M8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0830149</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000083">
        <property type="protein sequence ID" value="CAD8184610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W5M8"/>
    <dbReference type="OrthoDB" id="297072at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8184610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="123" mass="14360" checksum="DE513B0373C23399" modified="2022-10-12" version="1">MINTQSEDDEQPVSNVQEIQKPQKFSLFDLIVGLSIFSILFFQQCMGEILFPIGFIFLMNGILYKCNRRIDQFMIGLFYVVQLFAVWALTYHDFEECRIGYVLVVIYVVVIFCFITSSMIILT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W8M8</accession>
    <name>A0A8S1W8M8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0870013</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000087">
        <property type="protein sequence ID" value="CAD8186334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W8M8"/>
    <dbReference type="OrthoDB" id="10346461at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="25"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8186334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="336" mass="39789" checksum="1866615C6775C7DF" modified="2022-10-12" version="1">MEKTQQLDRMLKEKIDENLKQVNDIKVKELYIQNLQAEKTQLELQKKDQDYQITKLTNNFKEIEKELWKLKSIELNEQLQQNINQKVSKSQLIIQGKSFNNNSMDIEGQSFGKNFKQLNNNDENYSVLEYYIKQNKDLNRDYQTLLFKLQNFDQVSNQIKNSQNVINQNNEILLKIKRIVNNPNGISIQELKNIVNSNETNDFFNRYFKCCDGQNELVLCKQRICIYHLNCLYPKLIKQGQLALNNCDCGQPFPISILRKVNSVESKCILDQILDRQLQKLISQMIKEKRFLLYKCPNLFCNFQWFFRKDKSSSNQGSLSYCPNCQINVYSEIKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W9S0</accession>
    <name>A0A8S1W9S0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">V-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0840193</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8185145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W9S0"/>
    <dbReference type="OrthoDB" id="1719357at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15843">
        <property type="entry name" value="R-SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044565">
        <property type="entry name" value="Sec22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45837">
        <property type="entry name" value="VESICLE-TRAFFICKING PROTEIN SEC22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="211"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Longin" evidence="11">
        <location>
            <begin position="6"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="12">
        <location>
            <begin position="148"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8185145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="27206" checksum="4EE3E5AD7E28E688" modified="2022-10-12" version="1">MIFDTIIARANDGLLLCEVQSNEKVQLSLEVKRQIKLILKRGNLSSDVQYQQLDSQQSNVKAVYKTSMGVCYVVFYDFSFNLKLACCYLDEIEKGFQDELQIRYGTSNVNYRSKLETIESNDSYCFQQFERFIKKKKQEFQDQSSSKNLERLKMEMTELKKIMNENVKLLFDRDEKLQDMNEKAKSLKEDSKLFKDKAAELEWSIWFKKNSVWFIFGGFLLIFLFFKIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WC48</accession>
    <name>A0A8S1WC48_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0870153</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the KTI12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000087">
        <property type="protein sequence ID" value="CAD8186593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WC48"/>
    <dbReference type="OrthoDB" id="9972657at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002898">
        <property type="entry name" value="Protein CBG21524"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013641">
        <property type="entry name" value="KTI12/PSTK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12435">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08433">
        <property type="entry name" value="KTI12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8186593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="280" mass="32757" checksum="0C46338888AB5772" modified="2022-10-12" version="1">MPLLLICGPPASGKTQRALEIQKFVKEKLNKDSIIINEENLHLQKDDAYKDNTSEKMTRGFLKSNVEKYIQAGQLVIFDSINYIKGYRYELYCLARAAKITNCLLYLNVPLEICQKNNQMRDNKFNDNLLIDLYKRMEVPKQKDRWECPYFEVRFQEKTPIEEMIEVLFFAEKTAKDPVATKKEQQNDGNFVHEQEKRVQEILDQILNKQIECIQIGNGSIKFQGTKSSLILKRALSIIELKKLRLEFLNMLRITPVKTLEQTNEAFINFIQAQVERLGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WCS5</accession>
    <name>A0A8S1WCS5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0870155</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000087">
        <property type="protein sequence ID" value="CAD8186597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WCS5"/>
    <dbReference type="OrthoDB" id="299163at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08368">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8186597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="994" mass="118217" checksum="352CC53198B6D6E4" modified="2022-10-12" version="1">MELFLGLTRQQLSDDCYIEFPKVCNEIGTKWLIGYNHIITIDEDYEKYKDGIRKEQAQDLLIQDINTYKIVLEYEYNEKYGEKTFQSLNLGCQYLLLSIFINSGRVLQFSKLIYQCQQNNFYEALKSNGRNHIKNENQNIKLNIKCQEEQQFFKEHFDEQGIPKQEGIWKIEIPQTNEEDLYYAIQILDDRFYTLGLCSIFAINTELEFCNYKSVLWLEFLKDQEGKKYNFVSYNQEQQRWFPVNVMDQEYQIIGYSHICNPTEVLAYQDGLTDGQVNYLFLADYDQNCLKLRRFLNQEYQNKDFDNLTSQVQLLLIDLSFRNWPLIYYKQSIDLALENKIQEAINQSYIYYIPSESVNSHYLIRKDEIYTFLNQMSSTYQLKTENNNMFLQNPNLNNIVGVKITEDNFSFILKEEAETKIQIEDLITLLKVYYDPIQQVDDIAFSLEPEDNDEEMMRPFQNKIYSPEYLDRTNVGIVLYEADFLLKQMSLGIEVLEREPLITKPFVYGNLDIQPLYKLDPEYYAKLFLKIGKLKLNILEDKHNNVHKLEIEKIDIAVEARILNQQLNEEVIQDENNGAQRFASQFTQLYDQISQKYPIFIRLRQILMANYLAKYMYEKGIPINYEFIENIFYQKLISQEQLYRKSPTLKLYQEDEYHIIDLRGGIDGNSSQIDNIMKNALNQLDENQNLQVVETNSEIIDAKFQRCDSVSSISTQASLEVINELKKKEKCINCKKDLEQCEIDQNKICKACNNQKCSKCYKISNEILLKIQNEICCKECFKCYQCNKQNPNKIYDQKYYHQNCFEQAKKQFELKKQMISYIKKTFGTKQKQADNNDKQSYFQTELYPFINIRDYSGYIIGYNHPIIKSQAQVNLKQYKLPYKKAEEFLIKDLEEFENQLNQFYKVHNYTRDSNFKMLMLFILFILKNFDQVLNLANKSNYRDLFVEIPKLQITNTKGVHVLRGKIEKALDFFKQHFDQNGNAIQNQEQKIKNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WDB8</accession>
    <name>A0A8S1WDB8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">G-protein coupled receptors family 2 profile 2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0820026</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000082">
        <property type="protein sequence ID" value="CAD8183876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WDB8"/>
    <dbReference type="OrthoDB" id="299724at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 157-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 116"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05462">
        <property type="entry name" value="Dicty_CAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="14"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="6">
        <location>
            <begin position="15"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8183876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="325" mass="37815" checksum="4598714343E8274A" modified="2022-10-12" version="1">MTYDENTINQIEEVLIRIILITNTLSAVGATLMILTYFKFKELQTFAFRIVFYLNVSDLILALSNMLLVKKDFNNDDPILCLIQSYLINASQLSSIIWTAIIAFILYQSCIKNQNEQSFNICGYLFIGFCIPFSISIYPFFTHDYGFAGISCWIKEDIKHPVRSTIVRYINFYIPLMLIILYNSSVYIFIICKLYGISQQQTKFIYKMISYPLILIICWIFTAFNKTYEEIQDQQQIWLLYLSKALSGLMGFFNYLGYGFTPQVRDQCARYCCKRNSKSVSVELELKNQQSLDTSQNTEEIKSRNSVNSQRDSVQGELSFIAELY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WLZ6</accession>
    <name>A0A8S1WLZ6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">T-complex protein 1 subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0940171</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WLZ6"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03337">
        <property type="entry name" value="TCP1_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000017">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000069">
        <property type="entry name" value="T-complex protein 1 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000005">
        <property type="entry name" value="T-complex protein 1 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012719">
        <property type="entry name" value="Chap_CCT_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02344">
        <property type="entry name" value="chap_CCT_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="528"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8189727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="551" mass="60797" checksum="EDD349C7E0AD61F9" modified="2022-10-12" version="1">MQGAPVIVVNANTKREQGRKAQLGNIQAAKAVSDIVLTTLGPRSMLKMLLDPMGGIVMTNDGNAILREIDVQHPAAKSMIELARAQDEEVGDGTTSVIILAGEMMVAARPFIEKNIHPTEIVNGYFKALEDSLSILDEIAQQIDTEKKEEVMKALQSCIGTKFAFRWGTLISDLSLQATRIVLRGGNVNKLNLEIKRYAKVEKIPGGTLEESCVLEGVMINKDVTHPRMRREIKNPRIILLDCTLEYKKGESMTNMEMTKESDMTDALQQEINEVALMCNDILKHKPDIVITEKGVSDLAQHFLLKGNVSVIRRVRKTDNTRIARVSGATIVNRPEEIQETDIGTLCGTFEVKKIGDDYFAFFVDCQNPTACSIILRGASKDVLNEMERNLHDCLAVAKNIYQDPKLLPGGGAVEMEVSARLLEKANKVEGLGQLPYKSVAYALEIIPRTLSANCGADTVRVLTELRAKHSETGGLYFGVDGNTGKIAKMNDINVWEPLSVKKQVFKTAIESACMLLRIDDVVSGIKKKQQQSGRQGEEEPQETFGDQRDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WSU9</accession>
    <name>A0A8S1WSU9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0990047</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000099">
        <property type="protein sequence ID" value="CAD8191465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WSU9"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000334">
        <property type="entry name" value="Heat shock factor protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF206">
        <property type="entry name" value="HSF-TYPE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="4">
        <location>
            <begin position="6"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="117"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8191465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="249" mass="29872" checksum="E3C916FE4B620A74" modified="2022-10-12" version="1">MQEIKQIPSFLQKLYEILESEQKAIGWNKEGTTFQILDSSLLTEQIMPQYFKHRNYQSFLRQLNMYGFKKLKNKQGKSEFQHSQFRRGLKNNLLNIKRRNQDDIKQSLECLTKEFEQESYLNEHQKLRKQLVELQSNQRSLLEEIRRQMERNRNLQRETQDITERINTVKSYQLRKLNKLIIIIQKLPEDAQISKMALMKEKIIRKLELIEEENVSIKEISLEKQDSYMYQEPNLTGVKSPAGYSPKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WU32</accession>
    <name>A0A8S1WU32_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pre-mRNA processing factor 4 (PRP4)-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1010037</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000101">
        <property type="protein sequence ID" value="CAD8192231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WU32"/>
    <dbReference type="OrthoDB" id="540662at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030621">
        <property type="term" value="F:U4 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.110:FF:000015">
        <property type="entry name" value="WD40-repeat-containing domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014906">
        <property type="entry name" value="PRP4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040608">
        <property type="entry name" value="Snf8/Vps36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19846:SF0">
        <property type="entry name" value="PRE-MRNA PROCESSING FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19846">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04157">
        <property type="entry name" value="EAP30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08799">
        <property type="entry name" value="PRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00500">
        <property type="entry name" value="SFM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Pre-mRNA processing factor 4 (PRP4)-like" evidence="2">
        <location>
            <begin position="418"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="544"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="647"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="689"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="731"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="773"/>
            <end position="806"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8192231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="848" mass="97366" checksum="5966A0DE66B239F6" modified="2022-10-12" version="1">MEQSLFFLSAEMTAMKAFNEKTLSIYQNMQFLDKKLKMKIPKLNLAITTHRLIFTNHTNIDQLTVNDVKFVYFVIPLQHVKTIQPKTGFLSSKIDHINIETNLGEIALYGDNLEGPLLQIKGSIEKKEWLQVQEQQQTQSVYRGMKSEIEKKEKQTQQTIDTVQSTFKGGFEELFKNATELKEISQYIKQNIKKGENNEIDEILSKIGQSRLDKTEDQFYEKLADQVYKLCVELFPKMGGIISLLDVYYYFNKKRNSDLVSPEEILKAGLQFQKLNYQAKVERYDGISVIESTQYNSDKDYENTLGKYISYEIGLTAEQLAKKLGISVMICKIKLKNAINQGKVCVDNRIEERQRVTDNLSQISQLSTRAPTIINQQQESLPFISYTTENVDRQQKQQEENERKQKLMAEARKLMVPTQESQIKSQLRQLGQPVTYFGENAADRRQRLRTLLTDHLMQYGRLPQFFKQQNNHNMGNLENEYFLYEGVEQLRAARIEIAKLSLHNAALRVEKLRLRRLTVDALQEDQEVLEEMQKVNHFAIQMSQFGDSSGISRSTISPNQQYLATAGGSGECKLWDIQTASLKSSFLGHLTKCHSIAFHPQSLLTLSPQSFANVATASADLSIRLWTLDLEQQEVENLGMLQKSIVLKGHEDRINKVIFHQDGKRLISMGFDKTWRLWDIETQTELMVQTGHSRAIYSGALHPDGSLLFTGDLGGFGMAWDLRIGKGILPFVGHVKGILASDFSMNGYHIATGGDDNFIRIWDIRRRNVIEKIPAHIKLVSDLKFQPNYSKFLISASYDGNIKFWNSRDWSLHSVYETSDTKHTSVCITDDIKTMISTGLDRKFVIWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WUN8</accession>
    <name>A0A8S1WUN8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1050066</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000105">
        <property type="protein sequence ID" value="CAD8193834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WUN8"/>
    <dbReference type="OrthoDB" id="5592486at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8193834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="232" mass="26247" checksum="17C06673216D1C9D" modified="2022-10-12" version="1">MIVFCANSLPQVISNQGVRQFQVILQTLKPQLILNQCGGDIETRSQYFKKQLSKFNEPFHLIGYGIAGLDLRYVLSQNNNIRAKSLITIGTPHRGSILSDLYRRKRIEDDVIEPICRVLGVRQNYFEEINSENIRDFNLVATNKDVIKYFSLNAETEVGQMSQIYQASGTIIESEKEYPVESGSDGIFAHNETKWGQHVATLDCDHGAMIGIPNQKNGEQTVDIIQQLIQDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WUS3</accession>
    <name>A0A8S1WUS3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1020025</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000102">
        <property type="protein sequence ID" value="CAD8192537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WUS3"/>
    <dbReference type="OrthoDB" id="392073at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF8">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8192537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="363" mass="42679" checksum="A6CEE92ECCA7F637" modified="2022-10-12" version="1">MKQQKQFTCQDIEHEGEDIQGFCLNLGCQDSKPQFCLQCGVDPMKHSNCKKDLKGFGYISSFITKFNQNILDLTTQLDKSFVQVKQKYEEFSKQLEKIKLQLLKISQYLNQQDYNQMKENLQLIKEGYQYLNNQEEIKKQNQIGTQLINIKQMIEAWDLNKIQQQTQYIDHQSILQQGLQLLNQQKWQQANELLIKNIKNLEKQLSFAKFFQSISLIEMNQTGIGINMRDQAKKINSNLFKDLLDYSDQELKQNPQNAFILIAKSYALNDEKQFQLAIQQCDKVLLEEPQNIHALYRKCFSLFDLKEYEQAIQCIDKALISNSKYSIGLSMKGVSLEKLQFCLFFFNKQQKPILNQFFSLLFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WW10</accession>
    <name>A0A8S1WW10_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1050175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000105">
        <property type="protein sequence ID" value="CAD8194018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WW10"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="345"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8194018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="622" mass="73581" checksum="45F9F09A3467F7C6" modified="2022-10-12" version="1">MKQKSNNIQIQSQDRYFAYHKNQQYQIILCFKEKSSIIKDNYFFLQNYFQQEKEITFTEENGSYIYRGNLEVPESNRWEDLKLKIKITSQNNKVIVINLYQWDFNFYFNQSSIEKLINQSDNLVANENLKTLLGVDSTFQGTNSELNMQDILSAISHQKTQIDNLSDQVEEQFKINQKQLTGLATYIEQQNQKVMDILQVQSQAGNLLNLPSLINSDLCVQEKKIAEYIQQLLDVDKSIQNVFTQFNPKIKLTLIMHEHILNILSQNREFIQRINPLILQKLYLISRIISNFQIMAKSIKGQSGNNLSNENQQKLKSICKQMLKYKNLQKQFQNYFEQINFQFKNDELENQIKNLKYNEIQYQTNKKKLQQQISLLKKQVEQEQQKAEQFEKQLKKYQSQNNNNEELKKKNQQEKQNSILREKLNQAQKEISSLQIEILQNYQIQIHQGDQQENFLNKHTILDQDENCSILKFKLDAFKGGLLILALYINKLFKGNSYFGLVDKRNSQGYFNFMGKEIDQQFSNDLPVFFLDQKAFKITSNIIPTKTIFKNFQEEYCVLIFDTSLNSLSIYKKDGEVLSYKVILPYKEIEYIPCIIMNQNDKDDNRKDQLASLKFNFINVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WYG5</accession>
    <name>A0A8S1WYG5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Bifunctional polynucleotide phosphatase/kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1040195</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WYG5"/>
    <dbReference type="OrthoDB" id="19045at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046404">
        <property type="term" value="F:ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046403">
        <property type="term" value="F:polynucleotide 3'-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000078">
        <property type="entry name" value="Bifunctional polynucleotide phosphatase/kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000737">
        <property type="entry name" value="Bifunctional polynucleotide phosphatase/kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006549">
        <property type="entry name" value="HAD-SF_hydro_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013954">
        <property type="entry name" value="PNK3P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006551">
        <property type="entry name" value="Polynucleotide_phosphatase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01664">
        <property type="entry name" value="DNA-3'-Pase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01662">
        <property type="entry name" value="HAD-SF-IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12083">
        <property type="entry name" value="BIFUNCTIONAL POLYNUCLEOTIDE PHOSPHATASE/KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12083:SF9">
        <property type="entry name" value="BIFUNCTIONAL POLYNUCLEOTIDE PHOSPHATASE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13671">
        <property type="entry name" value="AAA_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08645">
        <property type="entry name" value="PNK3P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8193631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="386" mass="44376" checksum="ADA9B08A6A9722DF" modified="2022-10-12" version="1">MKQKKAQDWDWNKKGTLIYGTIDFKPQPLVLGFDMDETLIKTKTGKKFAKDANDWQWWNPKVIPTIQEYSKQGYSIVIFTNQNGIEKGHTKESDIKTKIESLQKELKIPLAAFIASSDDNYRKPRVDMWTDFQELTGTKADMKKSIYCGDAAGRVKGKIKDFTDTDLKFALNLGLIFKTPEQLFLKEDVDEDYSKSLGFNPKSIPKDGFLFKEQNVNKFTKPDKPEMIIMIGAPGSGKSTFVHNHLNEYTRVNRDALKTKEKCLKVAEQAIQEKKYIVIDNTNPTVEDRAAFIKLAKDNKYPVRAFFLNVSKDLCLHNDTQRDTNNFREHFSKKVGKMPINMIYSKIKPPTQDEGFSEILTINFLAGPFKNKDDEDAFYSFVHGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X0G4</accession>
    <name>A0A8S1X0G4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1100046</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000110">
        <property type="protein sequence ID" value="CAD8195633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X0G4"/>
    <dbReference type="OrthoDB" id="16262at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031956">
        <property type="term" value="F:medium-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000008">
        <property type="entry name" value="2,3-dihydroxybenzoate-AMP ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000003">
        <property type="entry name" value="Long-chain-fatty-acid--CoA ligase FadD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201">
        <property type="entry name" value="ACYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201:SF30">
        <property type="entry name" value="AMP-DEPENDENT SYNTHETASE_LIGASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="3">
        <location>
            <begin position="34"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="4">
        <location>
            <begin position="470"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8195633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="572" mass="64485" checksum="CC21FBF121905C73" modified="2022-10-12" version="1">MLQKLIRYYKSYYHGVSSIPLTYKTIGQQLQDITEKYPDNIAIISQLQGIDMKYSELYTRSKELAAAFIALGLEKGDRIGIWSPNNVEWALTQFAAAMADLILVNINPAYQSLELEYTLNKVGCKALIMKSSFKHSNYVAMIRELAPELDTPGHLQSKRLPYLKSAIIIDNIHKHGFYNFKDIFSLSNADHLHEVDSRMEKQDPDDITNIQFTSGTTGLPKGASLSHINILNNGKYVGGRINYTEKDIVAIAVPLYHCFGMVLGNLACINYGSTMVYPSDGFSAAATLEAVTNYKCTSIYGVPTMFIEYINEYEKHNLKYDVSSLRTGLIAGSLASEALMKQIINVLGVKDISNCYGQTECSPITCQNKPSDSFEIKTTKVGCPLNAEVKIIDSNGNVVPYDTPGEYCARGYSVMKKYWDDEKATRETIDKNGFLHSGDIATMDKDGYIAIVGRSKDMIIRGGENIYPKEIEEYLSHMNGVEQVQVIGCNDEKYGEEVFALIKMKKDAEEITGLDVYQYCHKKIAYYKIPKFVKFVNEFPQTITGKPQKFKMRQDINKELEDPKCVELYQIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X3L6</accession>
    <name>A0A8S1X3L6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GB1/RHD3-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1080162</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000108">
        <property type="protein sequence ID" value="CAD8195175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X3L6"/>
    <dbReference type="OrthoDB" id="2135133at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030386">
        <property type="entry name" value="G_GB1_RHD3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003191">
        <property type="entry name" value="Guanylate-bd/ATL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015894">
        <property type="entry name" value="Guanylate-bd_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10751">
        <property type="entry name" value="GUANYLATE BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02263">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02841">
        <property type="entry name" value="GBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51715">
        <property type="entry name" value="G_GB1_RHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GB1/RHD3-type G" evidence="5">
        <location>
            <begin position="44"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="481"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8195175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="602" mass="70024" checksum="72309E05A09B95E7" modified="2022-10-12" version="1">MIDLDQIKLEQINDYKAIQLITFVSQNNKFELNSEAAQFLSQLRGNIAFVVVCGKYRTGKSFLLNKLIDVQNEGFSVSPATNSCTQGIWLYTKPLINKQSNLQIYFLDTEGSESGVKSQTHDAKIFALAILMSSTFMFNSIGCIDEQSIIQLHLTTLLSKNIQVQDHDNSNNENILGYYTPKFIWLLRDFVLEMKDGQNRKITPKEYLELALHDDRQYQSENSKKVRKTLLTCFKDRNCFQLVRPVINENELQKVNQLPNNQLREEFQQQVIQLREYLLKNTSPKQLNGVNLNGRMFCKMIETFITNFNKGKVPIISTAWEHVVETECQQGLVQAKQLYETNLKKYFGSDEAKSFEEIFQILKKIRDESFQTFHYIAGIREKNNHFEEYKKKLLDFMTEKENLAIRLNDDMNNTKNEEVIAEVSQKLKENLNQETFTIDNLEVFFQHFNEFLTAYDKKSAGTEKANTLIHFLQNHHPIIVKQLVNSVVQKYSKNKNQAEEEKAVQQVKEKQLLDNIKILEQNIAQHETKIKKLDQDKVQLEAQKKQLSDQLSKLQTENKTIRDNIKDVDELQNMIAQKNEEIQGLKKIITSLQTKKKKGCCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X636</accession>
    <name>A0A8S1X636_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1130118</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000113">
        <property type="protein sequence ID" value="CAD8196737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X636"/>
    <dbReference type="OrthoDB" id="297634at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8196737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="623" mass="73894" checksum="EFAFD06EC10BD424" modified="2022-10-12" version="1">MNLITLNFEVSQIEKEFRESKQKLFSTILVYQKWIFGLINFLSFVSCLISKNWIIASISLGTLILILISIKVHKKYPYVYEIAIIVYILLINAYIALEYQFASIDWQYTEIFFISFTFASLLNLMNFIKRALLLLITVTFHLIFGIRHQDINADTYIRYLLYVILYVQFMYQFERLLRIIYLDYQKCQQFLNKISKFKDVQSYSVKYDERKSLILIEKNNNKRILKQDQEEFLKMITNMQLSMQNQKSRVAQIISVDNLFSPKKQSLKQLLVYYTQESNTDMNDTPSKTREDIQIYGFYQQKVFALSIFRSIDVQPITVLLMKESKSESQEEELRLRMRNNQKQLNYIQQIFDRQIKKSLIYFRWIKNFANKQTDGYLINKSLKMINYSLIKGYTDFLNLETFSLSKMLCPGIKQFDLILLIKDVFAFYDGYNKQKPESMSYIQQYEIINNLSSNSIFSDFKYLKLLLFNLIHYTSEKSQTITVILDEVTTEFSQTPIIRIKIQYQAPNLTKAQLHSLPILNPQSLEELKHNSTVPLDLDLAVSLILIRRLGPFDRMKLVQQKRKCNYLEFYIFQQLMEDYLLIPIKSMKPSDKIIVQEYSKIANSFSTSIQIESAGESFQFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X6H5</accession>
    <name>A0A8S1X6H5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1140041</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000114">
        <property type="protein sequence ID" value="CAD8196957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X6H5"/>
    <dbReference type="OrthoDB" id="303309at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="286"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="184"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8196957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="414" mass="47711" checksum="C396BA1020BA7AC6" modified="2022-10-12" version="1">MNSYLISKYSQSSHYHYKKGINDILEGKRCQLLIKLKDLQCYDTPQEHITKYYHINQLRDKLHMLGEYYKYHNDIPRLFMIPAIIPLNYFHDRKRRLAFFRIARLIAQENHNNPDKPQKGIVGDSPIPLTSEQITPQDPSSSDEILSKNDKILQGISFLIQEPDSIEKIKQQIQSMIKLKKQPNTSSTKINSIGSNTTTSKSNKQSILKQTKQPQPQKSKSPSLKIQSASIQLIDVASPRDQKPSKHISPKNIQQFTKSKPKSPSVNMASPVQQFIKLSAKMKQSFNKQQCSSARNNTNIPNSANQRKSSVTHILNILKSPNKNNSHQIQHISSTPRDQESKRSFNNKKVHQLELQVMSDLQSLLQKKTCLTHRSSMTNKNQIKKKLNTRELNQFKCIRLPLSNRDNNNNQQKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X926</accession>
    <name>A0A8S1X926_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1150103</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000115">
        <property type="protein sequence ID" value="CAD8197432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X926"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8197432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1005" mass="119395" checksum="07C96BC7925F9419" modified="2022-10-12" version="1">MFEEIQLSGIVPKEIASHSNITLIGNQVEEHFKCTDSSNTQFIEDIIYVVKVTNGQTVSIHKYDMKDLFHKSIQLTSEYLNFSHLITQNGCVIMRDYDTPYLLVVTQTGQIFYKNLISNASSIIKNLMVDNQRFMCLAKSLIYNNQLKFYVISDADQLHFITIDVRRGEIVKSKYQLSFGTKFLSKFFGTQTNDWKTAFQIDEKQIIHYSFQTQFHLLQIKGIDISSRHLQIEDNIKVKQIEVFQHKPNGQITLCYLHQNQLKIYSLIGNQLRINCIISQLVFLQNITFNISSIQNTIILIQGCEIYSLSNENNTFELVNQNKNPILGFINLNDQLLLFYKNHISVINQVQDQTSQKQYRLRQEIYKFKKDVENQYKLKDEELIVQSISIDSYIKNKFDQFQTLLKFMHRNLDQKKLIQLMKQQSNNFSIENFMNQLMDDLEDIYYYLKPTIQMLNLDFNQTQNQLIQQLNQLDQFLMCVYYDEFQPQQDFKQDQSVKLSLLKSLVINQLKDYYYMYLEIYLGLAVLYNIYKIQFELQNDHKLFYDTMLLLTLCQEKSYISFYDLLKKQFLIVSNDISLQKLSTIQNVIYRFISLIYSEVFRDFPIPEQLLCEAFIQNQKKSLINQNNAKEIIRYCKVSQQIGQQQDNIIQFINENEDQYQELKLRISFYLQQQQQNFQIEDEQEIILKKLIGYAIKQEQYQLLFQILNLNQTKQINDQLSLMIDSDQLFKCQLNARTTDQIAELLIQYSREKCHNLKKAEKVLQYYSFLMKQESKDKAFTLLFEYIVNLEILLFTLKYKDVARGVKTQLDYINILLNQMELQSKQDQLPYSKQLICKLSNQNYEQFKNPNIINDKSEIDSESPKNIRIVNQEQIIKFKKYKEIQNFVCTNYTPYAQLNKVIEQLILNGNLNQLINVFKLSLDQNFEKNIVFFYFSNEGQREELLNEVKPFLTQKEFSIMLAKICVVCNLNFEKCKALFKMAQSQEYFLQYMINVDKEQIASMII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XAR1</accession>
    <name>A0A8S1XAR1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1170058</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000117">
        <property type="protein sequence ID" value="CAD8198025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XAR1"/>
    <dbReference type="OrthoDB" id="303947at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="16"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8198025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="377" mass="44511" checksum="D005D46B02B5EE41" modified="2022-10-12" version="1">MKRASQIQIQKEAIVFSSDDSMNSDNSFNSSIEEEEAETDTNTIFHLKEQMAQFFTENPGLQEAIKFEQEEKEENAEKTEQNKHKQTLISKSEQQQFVHGWKLLGKDVIRNSQGGQTLPTLETINHRLNIFKQKVDKVDYLEILQRETQQQQQKQEDQVQVKQDTKQQPRSQSENPNRNENEEQKLEEEQDITYFSFKKKMQLALHKRQQLQREEEERKQRQQKEKQKKVNEADQLANDTPNQQTQNTLVGSKMEFQTHVGQMLYQTYLQTGGDKESFKKMAAIFLKNYHKKQKENLDKLKVFPPAIPKFTSEDCKHLNLLLPDFSTQLYAPNKQNLFQGVENYKFPANSPEQEFINLVDQIMSGKVMQFWKEMHQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XB70</accession>
    <name>A0A8S1XB70_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Malic enzyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1180061</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the malic enzymes family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000118">
        <property type="protein sequence ID" value="CAD8198304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XB70"/>
    <dbReference type="OrthoDB" id="25140at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004470">
        <property type="term" value="F:malic enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05311">
        <property type="entry name" value="NAD_bind_2_malic_enz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000095">
        <property type="entry name" value="NADP-dependent malic enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051674">
        <property type="entry name" value="Malate_Decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015884">
        <property type="entry name" value="Malic_enzyme_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045213">
        <property type="entry name" value="Malic_NAD-bd_bact_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43237">
        <property type="entry name" value="NADP-DEPENDENT MALIC ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43237:SF4">
        <property type="entry name" value="NADP-DEPENDENT MALIC ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00331">
        <property type="entry name" value="MALIC_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Malic enzyme N-terminal" evidence="7">
        <location>
            <begin position="15"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Malic enzyme N-terminal" evidence="7">
        <location>
            <begin position="283"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="Malic enzyme NAD-binding" evidence="6">
        <location>
            <begin position="433"/>
            <end position="657"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8198304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="687" mass="75815" checksum="256D2AD06E323435" modified="2022-10-12" version="1">MQPQQSLKNLFLRTNGLIKIKPKVRVTDNYTLSLVYTPGVGHICKVIQKDPDQLYDLTITGNSIALLADGSKFNLPRSSKMIPNLEAISALYKAFYDVDAYPIILDRNIMTDVSDYVLVMDNLSPTYKTIVLIDIEDTLASQILTAVENANLQCTVIISNSHNLREQLYDAALVKATLDARAILKPSQCEQIKKSITTIDFTNLPSYLTITLNKAYAQGLQEIYNNKWCHHTVSNIKPEIFINKLNDLYIYGEVAYYNKWSNDHLLQNNDFNQNSLELHKRYNGMITIELKFNPRSLEDLFRIYETSYQQDELAQLRQILIDDPTKLREITFKKNYAAIITNGTAILGLGNIGPSAGSPVMEGKSVLFNALGGIDLMPICLKEKDPHKLVRIIKCIAPIFSAINLEDLRAPDCFPIEEEAVHSLHIPLMHDDQHGTAVVSLAAVINYLKLTKKNVKDLKLVINGAGAAGVAICKLLHGHGIEDIVMCDTSGIIYEGRPQNMNNFKNDLAKFTNKNKIQGSLADAVKGRDLFVGVSSAGALTQDMVKAMNKDPFILALANPVPEIMPDEAIEAGAFIVATGRSDFNNQVNNSLAFPGIFRGAIDTRAKEINLEMKIAAAYAIANSIKESELSTTKILPGALTAEIPANVARAVAIAAMKTGVAKVNVDPEKVFDETRKLIMEGHLAGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XEH4</accession>
    <name>A0A8S1XEH4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1220018</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000122">
        <property type="protein sequence ID" value="CAD8199540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XEH4"/>
    <dbReference type="OrthoDB" id="286193at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="32"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8199540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="261" mass="30706" checksum="CDC7DE2032ADF567" modified="2022-10-12" version="1">MDLEIVINYEQNQQVVDDLCEQFDELEESNEKYHLYMNRGSRREMLDRYQVLNNHYAIFDGHGSSHVADYLQQNLLQRLQNEMSDEEYKKVFSDIDNNLTQYMQSGSVAILLSIQNKTVTITNLGDSKAIVFRENSFEQLNSIHIPQSKEEYQRIHKQGGLITKRQNISRVSGQLTVTRSFGDFYLKKYIISEPEIFRYQITENDRFIVMASDGFWDELSEQEVLQIIQKLDQNKNLAKQLYQAISSDYIRDNVTIMVFPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XHA9</accession>
    <name>A0A8S1XHA9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1240158</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000124">
        <property type="protein sequence ID" value="CAD8200471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XHA9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46818:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_125, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46818">
        <property type="entry name" value="DOMAIN-CONTAINING PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="114"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="708"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="708"/>
            <end position="721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8200471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="730" mass="85414" checksum="A06A48CA2FE71624" modified="2022-10-12" version="1">MFQDRYQNLFASPNEIATLTIDSNYIKKGSLRRIFKNVQYDTYENQQLKEFKNYLDSIPVEYLKEVQNDSVLLRFLYAHKFVKEDTVNYFVDHLNWLNNPETMNLEDIPQYSKLVQIIGRDEMLRPILHISLNLPLDDLFIKAITNKLIIMEDYMFVPGKVESWILIVDLSQTNQTIKPEKIESAIKHFVTNFPMSLEHIYFLEVNLILKQIGDNVLKLNEIKKLNLEDKITITDKVKYEKEKIQFLSPEYVHLFENDTQLNHSLQQNPQNQFSIFQQQPSQSSLLFPPSPNQVIRNQPNQSNQIPQQPIQLILNQPQPTLYGNPQKLSNGAELVGPLYPMIVYASTQPQPYLPEYHKKEFQIQTSTQVDPHHPIINNNQNNYSHNIPNNQNFLNTQNNPQQYQSDYSNPDYLLRTKYDDIIDQFNWNKYGVTQQLPSVEPEKVIHDVNMHISTPIQQHKPLMPTLQGTQQTIKKNDLNINQDIYSTYNNHFEDPILNNKDHFPLISEHHNFTHQDYQPDPINKYNKIDPPYDSVSPFNQYNKGFELDPLPYQKIDSYPPIHNQIEKKYDFGPIPNRPSEHSYPNSQIIYDKPQIPDRSSFDTPITTMNYTQPKYQSYVPQTLNNNQLNYLPSHPQTIPQSSIPQNSGYSPYYPQQPFSPIPSSTNLYTAPNYPIPTIKPSQSNLEQPKIVEQDGIKFQDETMVVGDNDHRQTKNSNRDPALDQQQCTIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XHC5</accession>
    <name>A0A8S1XHC5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">FAD-binding FR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1250020</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000125">
        <property type="protein sequence ID" value="CAD8200566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XHC5"/>
    <dbReference type="OrthoDB" id="432685at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06183">
        <property type="entry name" value="cyt_b5_reduct_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000021">
        <property type="entry name" value="NADH-cytochrome b5 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000042">
        <property type="entry name" value="Putative NADH-cytochrome b5 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001834">
        <property type="entry name" value="CBR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370:SF185">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 5" id="KW-0274">FAD</keyword>
    <keyword evidence="2 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="647"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="8">
        <location>
            <begin position="683"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="299"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="369"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="509"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="741"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="743"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="758"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="760"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="775"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="832"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601834-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8200566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="945" mass="112473" checksum="834F2F9E38937B91" modified="2022-10-12" version="1">MSKKQEKLKKELLVQVSRDVQNPQDSQKEAFKLVQNANEKFQQFEFDLKNHQMMKCQNKEVHPKKMSSLQKHSIHLIFYKYISQKQFILYIYHKLYIYLLYSKVQSRNRQIKINRKYKDEKLILQPQIKQNEIKAQLPKIQNQPQIRKANYKTYIKYQSQIREINSSNSRQKQLLKENQDLIRITNQRYLKNNNQSHIINRQLWSFLQKKFKGREIANKYKEILLKNQESAQQLQLQLNEQNRILKKQNQLQKILNFQTQMICFNKKLETRINRQIQIINYQRLIIRVQISICRIRKQNNYLNKAIDTFEQNLKQFESEVGRQQKINFQQTQIAAQDKLSILEKEGTIERLQKFNHQLSIDLNQINTLQKEVNEKFKSLLAAYNILENQKISIENDLEFRKKDQQEHLSCLKEKIQKLTNLKGIYDEELKKCKNQFKLQRLRDQFQLQYEQMKVLCFESEVLRKRLKLREVENDQLQFDNDKFKWSVRKDPNGILSDLKIMKLQPENPNAQVQEENDRLTAENKQLMKQIQQIELYVQEVKSMFDRERSFLEEKVFHKENEINEIIIKNHHEMAEIENKYQDIQRRQDTMKTKQDKPVNPINNNHIVNIINLIWFNNQALRSLFIMYLNYKRKLNPIFDLLKYYQMIYIYIFAALLVVALIKFIWKSLINPKGPTALEPKHKREYLTCQLIEKTKLSHDTYNFKFALPSKRHVLGIEVGQHIILHEQIKTREYPEGELVERKYTPTSPVDQKGNFDLLIKIYRANEHPKFPDGGKLTSWIENMIPGESIHITGPGGRLIYLGYGNVQINKMPQLYRKKYKKIVMIAGGSGITPMYQIIQAVATNNNDRTQLALLFANKSEQDILLYNELKAFAALKKLTLHLTLDNPPAQWVGFSGFVTKDMTEQAFGKLDNQTLALTCGPPMMNSLARTNFQQLGMNSDDIFEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XPM6</accession>
    <name>A0A8S1XPM6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1330010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000133">
        <property type="protein sequence ID" value="CAD8203063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XPM6"/>
    <dbReference type="OrthoDB" id="1726119at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8203063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="192" mass="22513" checksum="32661BF8E8A11160" modified="2022-10-12" version="1">MNPNFIDARIYRGIIGFSFNKGNLSLDVNNISYAQFDFEEVLKQDKQNLKALKGLTKACKMQCNYELGLKYALKVIMHEPKSPLGNFNAADCLKFLGQYNENTLQYINQAIDQEKRRYEQRISIYFRNKGKIIFYKGEILFGLNRVNEAREFVLKALEINQNYELAIKTLSIIYLDLGLERFDEILQRRTNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XUZ4</accession>
    <name>A0A8S1XUZ4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1400111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000140">
        <property type="protein sequence ID" value="CAD8205356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XUZ4"/>
    <dbReference type="OrthoDB" id="296687at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8205356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="285" mass="33003" checksum="EF80BDD988FCC072" modified="2022-10-12" version="1">MQLVRLTTNAHTPRNVPLRSPEKTQSITIQLPNYIFLGPKDNIKIVKFTKNISSTNIKQIRSNSPCKDQNMNLINNVGQIKKMHRKTTSIQITTQNPDLFIKLQSQDSSTTHKSSNIIALQTLSTKHKHHRSELTQDTNNEQLSTPNLTVPTLKYVEQSSNMSQGHLFNKSNKLRGQITFQNSFYYEGEIVKLSDALIIEGQGNLFTDNSKFYIVYEGRWKNNSFHGKGKYYNQYQIESSNDWHFNWKMVEAIFNKGEIIEGKIDFYQTEDTIAYKMENPKMKIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y0C9</accession>
    <name>A0A8S1Y0C9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1470093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000147">
        <property type="protein sequence ID" value="CAD8207451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y0C9"/>
    <dbReference type="OrthoDB" id="310886at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035856112" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8207451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="123" mass="15101" checksum="9CEFD391D6911407" modified="2022-10-12" version="1">MKHFIVFLIMFIIIHCQGQSLEQTIRQRYQKKQLINQTIFTQNQYQTRLLQQDQDDLVEHQEKIQDGEMLENQDNQKIKMKKIVKKRRIILKPIPPPYNEKEIHRKKRKVHKKIQKCKKIIQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y340</accession>
    <name>A0A8S1Y340_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1430037</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y340"/>
    <dbReference type="OrthoDB" id="299973at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="203"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8206114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="272" mass="32567" checksum="CAD38E1FAC48CD74" modified="2022-10-12" version="1">MLINKLPLLEQTQSPKPSHKTKLNFENTSYLQGDKFYKQPLVQKQKRQLSIMKSQNEIKKQIQSITDDSVSKRIQQPKTQLEFQTIDKLQIEFETKYIEQNRVIDDLKLENIKLKMQLEEQEQQIIYLKIINSDLRQQIEISSTDIQIKKIEQEYKDTYRRMDETLRKAIDENYESQIKMKNLQIKSQQLEQFTFMFKQQLTCKFCRKELNNTITVIPCAHNYCQRCVSGYVGRCFACNDDNKVQATYHNEYMSDLINMYKIFENILSLLRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y3L9</accession>
    <name>A0A8S1Y3L9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1430164</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y3L9"/>
    <dbReference type="OrthoDB" id="425490at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14811">
        <property type="entry name" value="bZIP_u2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="3">
        <location>
            <begin position="196"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="88"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="157"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="217"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="366"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="88"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="159"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="185"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8206364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="471" mass="55078" checksum="5C4C6FD54495A960" modified="2022-10-12" version="1">MADSFNSRQSGQFYNFVGTAGTADFQVGSCQEQQLSFGKNLLGYGDQQQGGSFQNNYLQYHAMDQDMKNSLDQFYEMKIEDADNQLIQEESSNQSRQKQSIQSERNSRSQYSGDQEYRLESAEQINSIFKPEVKLQIPQNQGKSIYREKIESLLEKRTSNNNSVSPSITPRSQKSQQKLERKRSQNLQNETSNEITDPTQAKLAKNRESAKNSRERKKIYQQLLEKQVSELQEENEKLKDICKNQAHSMEIVNKKTQKFQTFLEQQQQMFEKLELCIIKKVSDDEIGIIMDALRYRIQSNSKERNDTARVYFDSIAEILLPMQVKYLLYACSHSKDMFANSDQDYTEWMKGNFENTNVKFENLTKLKKFQNKVQQFKQNISNSLNNIKNEIKSIQDQASKLDQIWDALKSVMNPIQLSTMICSLYHNLYRNELQTSTLFEQLRNSQAEEDDFQFKIEEEINFGISKMVKRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y6J2</accession>
    <name>A0A8S1Y6J2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1550090</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000155">
        <property type="protein sequence ID" value="CAD8209645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y6J2"/>
    <dbReference type="OrthoDB" id="15189at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05471">
        <property type="entry name" value="pepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000308">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034164">
        <property type="entry name" value="Pepsin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF51">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="367"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="6">
        <location>
            <begin position="44"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8209645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="403" mass="46382" checksum="4264796E771CC6FD" modified="2022-10-12" version="1">MILYLLFQLISAYVKIDLLKSSPNHYNKHLRNDLLINNFKNMQYTGIIKIGEQDLELLFDTGSSIFWVFSNTCPSQSKRSSFDCQSSSNCLMTQEQYNVQYGQGEIGGNKAFDQLQISNNMTIKNFQFLIVNSQSNIDNLRADGICGLGLQEQFGFNSLINMLYDQHLIEKRVFTFFLNSIPEQINNSSVLFLGGYDTRYMSSDVQYVKLDKSDSWSVKLNNIQMNNKVLINDVSALIDTGTSLIVIPTHQFTQLLSNLRDQYKQFCQYSQYQIKCSCPDGDFSHFPDFEFNLEGDLKLILSPNDYIYMDVSVCVLAFTKSSNNYWILGDTFIRKHITIFDIDNKQIGFAKLATFEKQSKQIQINDYLYIFKVVCVCICVAIALIWIIRQVSCLGEASHEMYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y998</accession>
    <name>A0A8S1Y998_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1580041</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000158">
        <property type="protein sequence ID" value="CAD8210171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y998"/>
    <dbReference type="OrthoDB" id="1711136at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22696">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22696:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="292"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8210171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="346" mass="41494" checksum="12608E751D4003AB" modified="2022-10-12" version="1">MIILKIIVKDRQKFKMNQYYYQMLLPRIRIQFTLSEKTIKILDLSLLGLAILTCYKLYKCDSKRNEEKQKLKRFQYLYTADECHLLKIDSKQITVQGQINNDNQFLQLKSYQEQKKGQNILSNIYSSESIQIFDPQTKTEIIGKITKDCQFDLDLFNYHRQWKYQTFVNSESNFIVRQYKKIINYFFGKTLVENINGIDLNQYFIFKGILKQQESELMLEVDHIGLTPNLIYQQIKKNLEKYQIQEFILCMILTSLMYYFYQKMKEFMQIKVLKKELFQNKIGEIAEDSDLCITCMTNVRNILILPCFHLICCKECLDQIAKTHYQCPLCRTQITQLKQVYIDNIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y9Q4</accession>
    <name>A0A8S1Y9Q4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1620104</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000162">
        <property type="protein sequence ID" value="CAD8211226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y9Q4"/>
    <dbReference type="OrthoDB" id="329563at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007091">
        <property type="term" value="P:metaphase/anaphase transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558">
        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558:SF13">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 27 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="119"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="427"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="495"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="529"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="597"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8211226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="654" mass="75737" checksum="CF5CF73A1761B180" modified="2022-10-12" version="1">MIEEEGLQIKSQLEQQLIEAILDSLQNHMNQNAIFLAERLVYERDTEEHRSLLAECYLHENQPYKACHILKECKSEFNRYQLAVAYFRNKMYKEGEIALIGPSFSNQFALQSSNIPNGGFGHFLLGQIYEQTHRLDDAKIQYYKSLDLNPTLWMAFERLSKIGEPVTINKVFIDQKQKQYEISRQQSCNIYKIIGNSLKNKSNLTKSGQKEVDDVKEESLIIDNINKRQTQGVKPFSTTGVQTQIIHLDDSSNSQMKFSQPQQKKIGAKMIPTPGIGLQNYQSLLSQPFQLSQNPNKNQNTKKSEIQKVNCVALQSSSSLINLPQLLKQFAHPYQLWTNYSVEAIANFQKLPPHHYRSGWVLEKVARSFMDQVKYTDAEKIWKEMRQIEPTRLEGMDYYSSCLWHLKKQSELTYLAHSCLQISMQAPETWIAIGNCFSLMKEIDNSIKFFGRAIQLRKDYSYAYTLSGHEFSQNENFQQAKKSYDTATSLDQRQYNAWWGQGNMYYKTDKYDDAKKCFIQAIKINPNNPVLPTFLAMSYAAQGNHNEALKYFEQSEKLDPMNGLNKYQKANSLIKLDKYEQALNELQSLSQFIPKETAIYILMGRILKKLNKIKEAQNCFNMAMSLDMKDQAKIKGLMESLSNPNSEFNDDFDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YB39</accession>
    <name>A0A8S1YB39_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1510129</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000151">
        <property type="protein sequence ID" value="CAD8208674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YB39"/>
    <dbReference type="OrthoDB" id="300202at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="159"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="244"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8208674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="347" mass="41559" checksum="47A16830A1677598" modified="2022-10-12" version="1">MIFESIIYNQRHIFEFQYQLLDYQYQLNFKTQKNQQWFQNYISLYQSRGAPKNQVFYDGDKKNSIRTYNDNSNTRQGFKIGWDIDQTRKGDSKIAGKVGQDQRNSYIPSYQAQTYVPVVEQNQDEEYMKQLYIQEMNKKQELAALYKQPDQYYQDALDNYNNGKQYSNNNNPNTYEQPQLLQQSNKYDHYQQPNDDEELYSKYKQGNSGSKQQYNGKPPIAQYNPITGIAYENAYTHQRVQEPQLQQQQQQQQQDDALSQFSQLQQHKVTSMKNKTSNIFNTDIQETENINNNRQNSRIQNKRKELDDAQYKGYGQFYKNPIEPIPTQYREQKFVNRNMIDNRIFPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YC01</accession>
    <name>A0A8S1YC01_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1530012</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000153">
        <property type="protein sequence ID" value="CAD8209004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YC01"/>
    <dbReference type="OrthoDB" id="10528194at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8209004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="247" mass="28553" checksum="8C8E5B5A07D06D70" modified="2022-10-12" version="1">MEKSKQLVLYTVFGSIQVASIVGLNNNKQIFKLKTLVFTDSLTINKDESITIIGSQGQITFWHNLNQWKSFQTLICHNMAPIYSLSLNPSDTLLISCSYDSTINVIRKSDQQWVIIQIIQVEVHGLSLCFINDDYFVFQPIDLKVQVYQYQRYEDNFVKVKEIAIQGIEYHLDNSFFQYNAAKDILIKSNAQFLNLISGFENVEQLTKSSLFFLGNRISSTFSNNGDYLIVINNFEKKFIIYMLKES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YEC4</accession>
    <name>A0A8S1YEC4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1640039</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000164">
        <property type="protein sequence ID" value="CAD8211558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YEC4"/>
    <dbReference type="OrthoDB" id="293439at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="270"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="309"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="350"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="424"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="455"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8211558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="510" mass="60330" checksum="F97E0997B3D22E4A" modified="2022-10-12" version="1">MFVLLIIQQCYAQFNILTTIGGKNISIYENDVQNHQFSVQEQYQCHPSELFEEDQKQDSRIFYIQNFQSQAINLSIIPISLRPRDMQLINVTNQIEQQEITIEGSSISEYKINYECYQNWAFVELHVRYGKNILNFRYIKICEERLYYHDYPDLILAVMALILTYIGAIYGQKRFKLKQTQAKQQIDELESSESFYKARQIYSPQYSQPEQIIKMGEFQTTSDSAQIISTKGKQTNLIVRIVLYMFFVIGLLFTLYLWEEISIFEIPIYGIYFVCVQIAMTDLFIYIVSSSISPYIKLPLYGRVKLINLFTYMLQFVYTFFLVLTDNWVLRSFSSICLLFQLIRFMKFYNLQQLLSVYSVIFGISFIIQYKFQIEFPKLHFPNTFFLENPKLTCSDRNLLQLMLPCSLLTYTRYFRKQTRSHSILFKLGYLSYIIGVCLSLKSNGNQILQNLIDNFLPSIMILLTINVYGVAKREMKQLYQGIEPGLHNRAIQQQYSYELTIDKYNNKNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YMU3</accession>
    <name>A0A8S1YMU3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1680029</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000168">
        <property type="protein sequence ID" value="CAD8212424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="15304at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19670">
        <property type="entry name" value="UBR-box_UBR1_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003126">
        <property type="entry name" value="Znf_UBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38924">
        <property type="entry name" value="ASPARAGINE AND ASPARTATE RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38924:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_10, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02207">
        <property type="entry name" value="zf-UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00396">
        <property type="entry name" value="ZnF_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51157">
        <property type="entry name" value="ZF_UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBR-type" evidence="8">
        <location>
            <begin position="47"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="1324"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="UBR-type" evidence="5">
        <location>
            <begin position="47"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="916"/>
            <end position="978"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8212424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1437" mass="169622" checksum="53DF69451C8C6141" modified="2022-10-12" version="1">MSKTLTIEQIFSNTNQEIDNDYIFNNFISSNQQDRKEFDLSKNPQQSLCGAHVSSQNISFQCFDCAPDPHHMYCGNCFQLELHQNHRAFFKKEASGCCDCGDPSAIANKESFCKLHKEQVIDIANEIQKVKPDIREKILNFLQESFKIYFSKCEQAASLLSNIPNPFLIQCCHISHIINYKQVYKLINNKYNIKNAYQSILAANHIQDIIIDFLSWLCQDRTSFALLIAGFLKSQFSVGVTYFEQIIRYSELRNVLDLDNDHKFEKLLYVLNIDEKFRLFTQEVFLTQQNDLWRIYEIQSFDGAVIVEEYIQAFETFIQGGVFYTKEKNQLQLFENYFKKINTCNEKYTAYSIKAAKYFSMNSQFVSEEFQDIQFTSPTIKQILDMNTKLLNILNTCHLGNINSYYLMLDFFSLKFQYNYFIKSFNNTLSILFKNYQTQQLSIEEIASLNPDSFFLTALLKSYSFIKGFSSRDTLYQPLISETQIQLNLLYIESRQIYNIKQCLTKYLASPQITMMAKSNLINILFYWSYEFIRTNKNNKEQYNYLSEFYHNNNIHNDIQKDQMKTIIRLNYCIYRSGSLIDKFFIILLTYLYQQNVFQSSNQFKMFLLTTLKQNEQDLQFNLFNIFQRCVHNFISLLYTPLNDQVSNSYFAISKEQLESYDMAFIKFYFILYKDEALNQMIGIIQSRKYFSDLSQNQYFIQIIARIIVSDKSFSCVCQALYSQTNLPVSLSFTLYQILSNLFILNHYQEFSEIKSTFEQASFSTFNLEQFILNFCELDENINKLKLKSHQQILEYFQIQSQNQIDRTNNHTYFNPLLFLNNQSIAQQIGEHLSSQKREEYFIKFGNSIEQLIQFDQLKGVNLELMEFFAQDKNVDLIIQQIQPQANSSTEIILIERLLNINAYLKKDNKQICESLKQLQMNKQQDQQSLQFILNKFGNSNQILEEQVEGLQVKSLQNVKMNKMKAKFQQKIQQFQQNDIVQLDDQTNQNEKYDTCALCRQDLDENQYCCIPILIQANPYLKHFPIYPPDIRNEIMKDQLNLLKISIISCKHKFHDKCLIATYSCSQKQRLLPLWFQLQCPVCKSTSETRLSINKNKSIEQYDEFNNWLLLQCQNFGIKNYLKEKYGESVELKLKEIYLSLLCDLLMQLFMDSQEFIRQDKHIVFINILDYLFQISKNITINHFVFQNQQSIFLNLINLIIELIIQNNHQQKSLNDFLQDNQDQIKSKIFLFDLPLQIINQLIKCFALNTEQQQNQIVQVNQYIQQFNRIAMIDFVKNKITENLGFKFKSFYDNYFNKPCCFCKGFSNASKSSDQFICLLCCEKVCDVECKKLFSKSGNMNVHAQINHQGNTIFVSLKTGQIALLSTPLTCFGYKNLYQNKFGQLIKITNIDTQWEEYYINNKVLTDVAEIIISNSLKQIIKNQELNYNQLDIRGFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RW39</accession>
    <name>A0A8S1RW39_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0010526</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RW39"/>
    <dbReference type="OrthoDB" id="311195at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.132.10:FF:000001">
        <property type="entry name" value="leishmanolysin-like peptidase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000050">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001577">
        <property type="entry name" value="Peptidase_M8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10942">
        <property type="entry name" value="LEISHMANOLYSIN-LIKE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10942:SF0">
        <property type="entry name" value="LEISHMANOLYSIN-LIKE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23106">
        <property type="entry name" value="EGF_Teneurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01457">
        <property type="entry name" value="Peptidase_M8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035713288" description="EGF-like domain-containing protein" evidence="9">
        <location>
            <begin position="18"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="466"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601577-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601577-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8132268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="562" mass="62426" checksum="DDE979C243FCA0FF" modified="2022-10-12" version="1">MNKSTLLLLICLSLITALPKSRKLNQNTKRKLDESPIDISGTWEPLRIKFEYVTDADVETKEFLDKIFSISGTFLSKYLLIRRSSDKIQLPQDAPTQFQEFFEMGNELLSVEYDADLVFFVTTMDDSNDSTLAFCAPIIFDDKTQRPIFGAIGWNMAYSEVSTLTNAGFESQLATAVHEIIHGLGFVEDLFNKFYDSVTGEVYPDGGMVQEDDVVKIITPRVVAFARKHFGCEDITGVPMEQEGGDGTAGSHWERTLFYNEMMTGNDMVSDFVLTDFTFQLLQDTGYYRLAEYSPDILTWGEGQGCSFYDQMCESEFPEFCSDEGSTGCAITHNGIGVCSSDDLSNSCKYYQIDPNLDCRNANSIGYADNGATFQDFGYQSMCVIGGFAATNGTPQDFSCYEYSCDGKFTIKVNGQSIDCSSGGVKTLEGMSGSITCPENFEQFCKNKDECPNQCNKRGFCMKGLCTCYGNYYGQGCELENCTKIRKGTECVDSCPSGYYLNDNINYCIGCPGNCASCSSYNACTECKDGYELRGGFCDYLEESSDSSHIKILILVLIALMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RYD1</accession>
    <name>A0A8S1RYD1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein uL15/eL18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0020185</name>
        <name evidence="7" type="ORF">PPENT_87.1.T0680215</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000068">
        <property type="protein sequence ID" value="CAD8178001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RYD1"/>
    <dbReference type="OrthoDB" id="297604at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.100.10.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000039">
        <property type="entry name" value="Ribosomal_eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17135">
        <property type="entry name" value="Ribosomal_L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="5">
        <location>
            <begin position="2"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8133016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8178001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="178" mass="19995" checksum="4EC432B46A4DC212" modified="2022-10-12" version="1">MGVDAYKKTRVQRNVQRKVTSTNLYLKLLIKLYKFLARRTDSNFNATILRRLQQTRTARYPISVSRLVKQINTAKDKTRTLVVVGTVTDDVRLLTVPKINVCALRFTETARKRILAAGGKVLTFDQLAQQNPTGTGTILLRGPRVREELKHFGRAAGLPGSHAKPYVSHTARRGKGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S152</accession>
    <name>A0A8S1S152_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0030040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8133916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S152"/>
    <dbReference type="OrthoDB" id="443402at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="3"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="154"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="334"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8133916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="515" mass="61382" checksum="9E277806CE164B4C" modified="2022-10-12" version="1">MEIEKWKTQCRYLQNELDSMKQQLSDAKDLIRLNKEQLKICYNPQLFDQSSECKAALVLLKYVQEENEVLMKKVEQLSDERNLAFDKAFISDQINESNQRIESSLITSLNKTITELKKKLNEQVQISNEIMGTIYLEKHLTQPEQLTLNFNNVIQQMNSMLLKQKLQIEELVEQKNDLAQLNFSLSNEILKIRTQRFTPRNQTKTFFSQYEDPSKFINNLVQLQKRLFFDDDSQDEKMHMQQSAVFSPLFSPSLPQKVKRQQKSQIPKDFKQEPEKHIPKLDLTKAQQIQQFNIKRMQLLANKLANEGVEQKIYMLQDELLRTKNKYHAQLVLNQQLDLQLTEWNIQMMELQNVNETLIKSNQMYEEKWSSIYQQFIFYKEFYLKHKDQIQHNLQSYATSDDQQQFSVFEFEKALNSSKIYKKNPSQKNHLNNNPALSIINYQEDTIQQSSYRLPTDRQEEVNKFLTQQYKCCLMMMAKEVISLDYQHIKPIQTDNNKVKIKRSYSNTIEYFPVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S235</accession>
    <name>A0A8S1S235_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Prenyltransferase alpha-alpha toroid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0020523</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S235"/>
    <dbReference type="OrthoDB" id="5428259at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005953">
        <property type="term" value="C:CAAX-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004662">
        <property type="term" value="F:CAAX-protein geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02890">
        <property type="entry name" value="PTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045089">
        <property type="entry name" value="PGGT1B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="Prenyltrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774:SF4">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE-1 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Prenyltransferase alpha-alpha toroid" evidence="3">
        <location>
            <begin position="5"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8133642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="297" mass="34008" checksum="226B92EBB705CA79" modified="2022-10-12" version="1">MQQQLTIEKHEEFIKKTLYQQKLPCKMDSLKALILYCCVTTLKLLKSEYQCEPIVEFLKHLEVQPHQYRASTFDDSEPSIVNSYCAISVLKQIKQKIEVNKESALNYVRSLIQEDGNIRSSVSSNDADLRMVYSALAYLDLLEIDTSEFQQTVGKFILTCQNQDGAFGLRPNLESHCGASYCAVSSLIILKLEIPNKSSLIEWLVMRQCKMTGGMAGRINKIPDSCYSFWIGWNLRLLGLDLLDKERLMEFLQHCQSNYGGFSKYPQSMPDPIHTLHSLLGILENEDYDYVHAILLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S276</accession>
    <name>A0A8S1S276_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribosomal protein S9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0020555</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S276"/>
    <dbReference type="OrthoDB" id="10254627at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_uS9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US9M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8133683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="700" mass="82392" checksum="44341DE390F382A1" modified="2022-10-12" version="1">MLRLVSRMRFCVAQRNFFVVPPMYPEKEDLAYRPKITSYDDSQKQLHAVYKKKLSKIKARVTQRVREMQPKQEIKLESNSKNGQDLLQNVLKHVYIDEETNCPLGLFFDDFVYDIKMKRGQQYEAEYNRKVQGLLSQMRVMSETELLQTSQDLLVKSEAPEYIAKARKVVFDRFLEGIKKLPEEERKQILGDDAQDFDKKLTDEAILNKYIDTNSVNAVAEEMAKENLQKDLQDFQIYDPKYNDKNQQLTFGKAYLKELDFYESGCTVEEISDDERLLKMRYIRPSDGQEALQTEQSIVDKVNLNFVKRAYLKRLADKSQEPKEPKKLINAKDQEELELKRLRKYFRNLDKQSLTLQMKAKQIDKRINDPVKFQDIVNNIDFLLLSAPKFIKDLYKDPNNKIKGYESVFQDLEKTKGQSLFWKDFKSSFETIFPEIKLNKQQDTFVDIKKQPYNRHVKELFQEKVDEVKQDEYQFSIELYKEFQQPSNLEFFHHYLTNIFKLNREQINDGLQAFGDNTNPYTYEVPSTAMMADDVDPDFKDVVEEEVKIEDFKEKSAIQIDQLIEKDYSESSLLYYVGRGRRKSSICYVQLKKGVGEVTINGKNLINYFTHPFYRRTALRVLELAHLSCLVDVNVVIRGGSQSAQSQCICSALSRAIVKMCPSIKGIFRKLLLTRTDSRQVERKKLGLYKARKKYPYSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S2G6</accession>
    <name>A0A8S1S2G6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0020227</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S2G6"/>
    <dbReference type="OrthoDB" id="291621at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035882876" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="499"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8133094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="571" mass="66536" checksum="34A62FBDD8FC92B1" modified="2022-10-12" version="1">MNFLLISLIIQFISYTLGFTITEIEEPLSSIKIHPNEHRTLILDDAKDAILLSYNHSRGCEVTPLQLQFETQEFRIIQPQDFTEKDFESDTILNFRQFVSMVHIKDGMLAITSDSIAYLLKFNYNTVFEHDFEEKGQKFARILWKADLQTIAPSLVAKNELPQLLFAPSYNLAFLLFSDSAQLFSMEQMESQTTSLDVYQVTDWRKRGYRGLTKEIDGLVFSAVGRDGLDVYKIIQKDLRFIKNLKLKDFGLNSSIEIVDFAIVKHGKLDGYYSMFLLDRECGLLLIDVHKDQYVNFGLRSWINFQSGGISVDTRNGRNVFMAYQSMNHHYVLEYNVDLDNGNSFQIRKKKIDHRIIDLDATEDFVIVQGVNQHLILFSNGYDFVMNNHQDQVFKHLGLRDFAFFNQSYKLEDMDDITKEYQYDDFFFGITAQSAFLTRFQMQPIKLNCFYHQTDVGQIFTYILQYNTTVEGVSDLVVRHTQKVNIEIVKTYFYEAEYYLLYSIGFLLGSVLLMGLGYIGYWFYSNKREHQILVEYIEKTAREKGIKQINGSTIQKGDETSVFQQNKSMLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S2X7</accession>
    <name>A0A8S1S2X7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0030366</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S2X7"/>
    <dbReference type="OrthoDB" id="320598at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="357"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="36"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="130"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8134556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="380" mass="46255" checksum="E12D07A2EB6BB1F6" modified="2022-10-12" version="1">MILTQQNYDGYKEYDINRKLRDEEKQTRMMKQEIMKQQLIEQQQLQEQRKKELQLQKQKEDEEQIQLIRKKQGEEKFQQIQNREATKQNLQHQNYLIQNHQKLLIDEKKQETLKIENDIINNAINGLSLEEQMRRQKREMQKQIVEQQMKELQQRKEKEKKDKEFEQLIYKEQTQNESHRIQKQEDSYRNYYQKLSERQNVLQDIYRQKVDNPKMQLDYIIDQQVKDRQNKEENEYLTKRQLQQKQKEMYQNGLANQIQEKNEKKKLEDNIKEQRKLEILQASIQYEEQQRQDRLKKREVQQQYHTQLSTLSQSQSYGSGIMNTEISQSNIYNPLTNPISSQIQNPYILRQIQHNNSSLQQSPSMNQSKLASFGKSSLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S3X9</accession>
    <name>A0A8S1S3X9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">40S ribosomal protein S14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0020483</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S3X9"/>
    <dbReference type="OrthoDB" id="308517at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.80:FF:000002">
        <property type="entry name" value="40S ribosomal protein S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_uS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_uS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018102">
        <property type="entry name" value="Ribosomal_uS11_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007176">
        <property type="entry name" value="PRK09607.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S14/30S RIBOSOMAL PROTEIN S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002131">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00054">
        <property type="entry name" value="RIBOSOMAL_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8133594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="166" mass="17929" checksum="E281B5D6DDE16693" modified="2022-10-12" version="1">MADAKPQSVQQPKGKKGKKTGEETVQQVVATLGPNVAGNELVFGVAHILSTWNDTFIHITDLTGRETLARVTGGMKVKSDREESSPYAAMQAAKDVYEKLKTLKINALHIKLRARGGVDTRQPGPGAQAALRALARLGLKIGRIEDVTPIPTDSTRRPGGRRGRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S5R2</accession>
    <name>A0A8S1S5R2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0030567</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="27537at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050349">
        <property type="entry name" value="WD_LIS1/nudF_dynein_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44129">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="NACHT" evidence="6">
        <location>
            <begin position="1285"/>
            <end position="1453"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1952"/>
            <end position="1993"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1994"/>
            <end position="2035"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2041"/>
            <end position="2071"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2085"/>
            <end position="2118"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2563"/>
            <end position="2604"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2756"/>
            <end position="2797"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2804"/>
            <end position="2838"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="37"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="534"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="622"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="803"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1095"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8134913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2943" mass="346404" checksum="4F6841F5DAB76608" modified="2022-10-12" version="1">MVLGLFKLIDIPIQSCCSSLKSVQNLTKRIGQEKLRGGGSSICSTQQSRPNKPNKQEENQSQFELPSNFAEQLRKHSNTIYEKSNYTLEKDSKKQLNASIQWFIFNKYYINSLVYNEDFAQKYSEKIRDAVALIIQAIPNFITMSCFLLHDLLQILNELLRFLVSQKFKSLIEISKQKKVKKILIEPQLQKTFLDYISKAKQEIKALNKQIWETGISFELRMAKAAILNFETNEGETIFKQLVQHTVLSFINMQPSIEFVTASFDAGKYLFQLAQSKWRNKQYEFYFFFEELKWYIIKRLHENCTLDSVQNQLSQSYLKNIQKSDNWLVHFCWVRTLSDLIFYRPLVEKENLFPNMQNKYEQNNLWDNEINTGYLLRSQFDENIAQVVFFDKYEKKGIYEKKGIYQRFRNLESFQLWLMYEYNPNAKVETFKSLLDYVRITSFSDQSKNDDRMNNRILFGNPNIKNLKNNINKLNQLIDNNKKIIEGSNNQQLVNNNLIQSAILCQEIYNQFYLLQEQTEYVSLLIKYTNEKNEKNGKNETNEKNGKNQKNQKNEKNQKNETNEKNQKNETNEKNETNQKNEKNGKNETNEKNQKNETNEKNETNQKNEKKITLEEYNFNQFAKYLVDKQEVQNNLENLNKIIEDFLKNVKERMKHQQNKETKLLDFQILNNKTESKIEPIQFKFEEILKEIFSFTISKQFIQGISKQYKIIIAQNQRDIDQLVYLLQGLQKVVKYLLLKYQSLKQQVQILLDNEDTQKREIDQKHLKNIEQNKYIRGFFREELEMLQQLTSSPSKLFDSDKINDMMNTISQHIEQAKNYKETLLPNQYIQYAAKIRLLLSQLKYIENVEDQLENLTKILDMYKKKKKEFIPSTDNQQSQKSSYERTVSNVAQNTNSHHEILNKILEKENEINKFYEIISRKVEEFQNYQEYIRFINDHEDQDYQQIVTFENDTIDKFEKETLEHIIKDEFYNLTITHEEIYSNRFIINFFECMANILNQNNPNDFNELSTTYVKDSKFLKQDSSDDYKVRECLFFNIMKGQIIIKEQHVVKFCQLIIQQLWMEEKNENVRNIFKDKEMIDQQKSLYSQGLNSLSKQLQQEFREKLKALQKLEAEIQHEGVESEKSLLKKQLEQKSKDFHDYLNNIEEMSSQINLSFLFLQEIHKDFQILQQKIDKIQLSVDEILIDLRVLRGKKYIELLKTRKKKVQNDQQITEMNSIYIELMTNRSIYESTSNQQNSKYRLKGEIKNSYLLIESQDESNQMNLSGDVNDFLYGKQNKQDQHFDVMLIRGQAGSGKSRAAKKIEEYIWKQYNSEQSNWIPIFVSLPSLKDPKTNLFNEALESDYYHFDKIQIKEFKDAICNERANVVFILDSYDELKDDVKKQNLYATNEFAQQFNIEQNRKNLKLIITTRSEILQEKYFLRWFTGQKIESLLDIELKQFTDQQRANYLQQYSILNIKKKIFGLYEYVQQIEGKKVDPEDVKVILNQFEQLLNTFQNDQTQEQNQKQQFLTQHKAQIILDKISSISYFKYVKEEQKIILQKELIGLWSKQKYQDSINNLQMGKILQTPYMMEIVVQVLPNFAKIYSEASQIKEMFKQNYMIIKRREQSSQNKILKDNKEKKDEQQLKQLDLFVERLDQIQFFQNFSILDNLSQISSYKLTDEEKKVVISAFKLKKFTIYEFYENFIQYYHSQQIQKQYRLGNVHNFESLAIDLWDFSESLALEMTVHDQTYFSHKVQGVLKLKKAQECIGPEQNWSDQYFNGELEEQQYKKIIRSCILLSCKGNVYSFTHKSIQEFFVSKYIINLFDNHFEKKKLQLKDLEQSLYNKDIFNTSKIHFQKVCEFITNFFQTNEDKIVNQLKTILNLSKNNENYKRAASNSVFLLSQFKTYMGEQDFRKIYIADTKINGLSFFASDLSSSRFSGVEVDGCNFNLSKIENSTWAIICNEQRTLAGHENKKITSLLMSPEGKTLYSSSEDQTIVVWDYTKRKQINKLQGHQGIINCLSISSNGFYLASGGTDNTIYIWDCTNENKTQIKTIQTIKDHQGSVNSLYFSLDQTILASASSDKSVLLISDYQKQEQTKKRFIKHKEKVMQVVVFPDSIMLATRCSDCTVTIWNIGENVPNIIRVFLKLPEPITCIALSHDGTYLMTGCKSVENQGFIKAWEITQENQRMESENFQFPIFCLSICPNSNLIAAGSKDQIILWEKFQFIDHKNTNPKIIQCKGEIISLVFSMDVYYLLSLNSKQKMELWSLKNQIVKHSFQLISAFLIGFIGKEHFVAVQTSKKITVEKDLDMIQICEIQSGRSKCFQLQDIITAGQIFKQPLHIALGFQNGVIGIWKWTSESYFDKFDQEHDRKIINISISSDDKTLISIDKYQTIKIWIAESKQCKSTINDIQNPINKIQIYAQMMIYVYQQEKDDIIRFHDTDKNEIIKEFSGYSGRISQLVFSQNNQRLLFIITNQEGESKIGLINFLQESKTYKGDGCEFSQIVISQYDEKFAGIGENRSLNIWDFQDQKLQESSISQKVGITSMTFTRQSNLISGQEDGSIILWQKKGEETIKMFEGHSDFVQDICFSPDGLTLLSCSQKDNVVIWDMEKYKKRTTIDKQANQVMFFPTYPSEQLLLLKNKQTIAIWDIIEEQAKTLPSLFESKQQQRDFKSQTEINYYLEYITDVTISKSGLQIVCGLSNGDIKLFKQDQQQWIELEKTHISKINKIQFQPCVSSQEDENFIASGSDDLTIQIWKNDGNQYNPFKKLDNHKCEVKALTFTSNGKQLASGDSNGSIIIWETEQYQNYNTIEGGAEIIAMAYSPDNSILAFGMIDKIIKLWDIKNEKAIQQIQSINEQIGSIVFSIDSILAYSFGQEINLCKSQYGQDIGNNENEKQYFPFFKFAKEPILQAEQCFIQNSTIDDDTIEGQENKSMIWLFQQKKAQVKEANDDINKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S792</accession>
    <name>A0A8S1S792_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0040505</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S792"/>
    <dbReference type="OrthoDB" id="303704at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8135948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="133" mass="15471" checksum="38838996D420810D" modified="2022-10-12" version="1">MKILDDIKQQVEKEMLKFDPRLAEKKYLKVTQSIKDLFLKAVVKEKQTIKAAAHSIGINYSSAKAIWAEFRNKNQKKKKKNKEDSDQKNQLVKRCSYKILNGCNKIKKFFMEIKSSVSQNLSSTHLINLESHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SCU9</accession>
    <name>A0A8S1SCU9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0060103</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SCU9"/>
    <dbReference type="OrthoDB" id="285802at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20528">
        <property type="entry name" value="CYCLIN_CCNJ-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000165">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0195">Cyclin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="2">
        <location>
            <begin position="80"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8137460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="344" mass="39922" checksum="06CC18F2CFA0F2A0" modified="2022-10-12" version="1">MDYQQNQCQHIDLIPFNNKFFCRSCGIHMPEDGSIGIKAMNFCFPGYLNPIQNLKKQWNRAVPIGSLPNEYQKQRLSLIDYMIEWSEKLKLSMNSLFLAVQFLDYFVSQKKVDPVQYRLYGATCLMLAAKSIELDERIPFISKLRRYTYLPYATLDFRRCECQIIKQLNWNLQCTTLIDWAEAVISLGIVYQQDELAQSENILKEKSTNILQQQTNQQIKQDIFKEHLDTVFKQPITAHKQINEKVQEQLIRLCVSVLKDGSYLDKDPAELTVSVVGCARKASGLKTSIPKCLNELLENVEPKFQEGLTDHFIKQVKQPTSVLGRAFTTDLDFFSLQNYKHRIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SF01</accession>
    <name>A0A8S1SF01_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0070104</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SF01"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000085">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="220"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8138416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="519" mass="58519" checksum="81C22067CB1ADF38" modified="2022-10-12" version="1">MNPVRVFPLKFNEQQNTQSTSSSPQLKKKQTPKLIEKPLLLNMSPILNGQSPIKGKFNVKRLTVNQKQEDKFQFTQQQRPLQSTSSSPKKRVISGATNVESLSPMKLPNLRRSDTQLNNNLQIKDETVSEIQSSKRKDTKTDGTPSQLIRGSIIKLISDSTPNSRPSVSRAKLASQTIDQDIKPKVSNQMVMLYGRSTSQAALVNTFIVDNEMQDYVNLFYSKSQAGQNGQKQTKTNQDSIIVTNNLGGIKNRHIFSVCDGHGVYGHYVSQLVKKLLPNIIDQQIKSNIGMQEKDIGENHYTDINKAMTQSFLKMQKDLSNSGIDITFSGTTCSLVLVSGPHLWCANIGDSRSILIQQQNNQKWKTIELSNDHKPDLPNEYKRIISSKGRVEPFISENGEQIGPPRVWLLHDQIPGLAMSRSFGDYVASTVGVSCEPEIIHYRLNTNCSFLVVASDGVWEFFSNEEISKIVIQYWQPNMTAKRIDEICDHIVKLSTQRWHQEDEVVDDISIVIAYLQKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SH75</accession>
    <name>A0A8S1SH75_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0080272</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SH75"/>
    <dbReference type="OrthoDB" id="10258888at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24124">
        <property type="entry name" value="ANKYRIN REPEAT FAMILY A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24124:SF14">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_25, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="312"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8139743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="409" mass="48824" checksum="ED988F3B67FE4474" modified="2022-10-12" version="1">METHEKYFYYTWKDKPKQSQLCSNDKKVNVLCQYSEQLLQSKQMNSDQKHLAFWQLKRKNSIVEKYSQSQINTTRIKTRRNSVVVGYESQIKTERKSSQTNQISQVSNTLTNKKQFQEDYIDELSRRRLSRFLSNYEICNLIHKRSLSRQDNQIYAEDIDKEIVTAQIKSIQNQQIRFQFHQSNKQIIPEKKYDNQINDMINMKQSTFKSVFQQIQHLTNEQSKKRMPITCHKMLKSRTKLVSCSHKVRERFKNIIKQVVFVAKYKCRVNFWFEYPIKYNNSFYEQIIEGNYDRIIFTLSQEPYLIHSRNKDGQTPLHIACICNNALLVKLLIKNCSNIEALDNKQLSPLYYAFKSNSNECFKILLNFKAKPWSPPGGKLDQNQMNVVQKNLLMHARLIYIFTIWKIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SH97</accession>
    <name>A0A8S1SH97_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0070454</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8139066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SH97"/>
    <dbReference type="OrthoDB" id="5979581at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000616">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="10"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="336"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="345"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8139066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="450" mass="52814" checksum="9A0334B9E343CE89" modified="2022-10-12" version="1">MKQLKINNQFVTQKKISQGSFGIVFQGIDQKNGNQVAIKVERPENEHLHSLDKEIEILSRLSNIIGVPQIFYHGWEDSYNVIVMELLQKDLASILKQRKKFSLKSVFQLSIELVLILEQIHKQGVLHRDLKPENMMLDDKNKVYLIDFGISKIYIRKNGILMPFKDRLPFIGTSRYASIAAHKGFELGRKDDLESLFYVMLYCLKGNLPWQNIKHVPDDQKTQKIGQMKENIEVKELFKDLPIEFIKIQEYLRKLNYASEPDYKTIVNLIQQAAKHANIVMDHKYEWETQLFNYDKNMNRYGTLQIDELPQKQYEKFSSNLLNCNNLLAQTPSKFQQLQPPTTKQESQQNSYSNYGSGNQNLSIYNSMGIIYQRSIEEISEEVQEPIPDELKSHNKIYLQTLPDQLKKPQPLFKTTQFDDYEYYKEDNLLSEKYHSLSAQITTIFQIHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SMK8</accession>
    <name>A0A8S1SMK8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0090235</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="327952at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="237"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="261"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="863"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1044"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1162"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1407"/>
            <end position="1432"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="806"/>
            <end position="833"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8140696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1466" mass="174121" checksum="FB4B6FDEA7DE490D" modified="2022-10-12" version="1">MNFTYQNFVHVLSIQTKTKIGVIKMLMTIEFVQNVALCFPKHGWQQLKYEDKMLSSIQQLTNYLLFLNRNTTEYLFEVFIISLSLIYFILLILYFHFPKSQKVSQLITITLNIFQKIFRIPLIVIYIEFMNHHSNSFLEITITLLIFVVFLIFLIATTYFQRDIAINFSKIYFPLNQFYTPYLQVIYILDLIRILIFVIMNTQTGLIIFYILTIITKIIAQYHNILQQNDPENFNQGLIFCNLIISVFLLLGLSNKINENLIFNLLLYSMTLVFYLSIQIDLFQFIKSNNIVHVIENKLQLNFNQFFLKQVVQLKSDNAVYINFIKQIIQNEIQQNHYLQNETNALILFDYLTKNQQSYFQALFILNKFECQQMKLSFFYKIYSQLFKNSIQNEIMRVNKSTLMIQQLNRDLLIEIEYQKRFSDILNKQINLYNNLIQGSKGFAETENKIIQVSQSILNTEKWLIKNKIQNNHTNIIYLKIQISFSSIIMQNYVQALKLKKIIKKILENDHKTLEALQIMKSKAFVITVSMIKQKGIILNVNKSMLQFFGYNKDFKLEILEQLMPEAIQPFHSQFIERFLQRNSLKNHYLQNSLEVLQQQENGKIQCLEINFTISNDKSDFSLISLLKKVNQDKGYILFDSSGKITGMNDYLEQFNGLYSQMKQYNYIQYFSTDLFYIIQNNEYLNEKEITFIQRLNIYKIDKQYQNFIQSQVKFNNQNNTPNLDYIIVSDRSDQYQLLSQKINYSNHDTFQFDFLDPQLQEKLIQIINLNITENDVALKCKVKLSKIELKGNIIQYQLELIPLEAKLENEEVENNNDEILQLENIKAKSQNDVSIVGSEQKGQYILHDILLKQSSSQPFISIYFYKIILVIQVVLFMIIQINQIDIDFNTNLKYSQYIKAPLQINQFYNKAFNFGFNQIIYQKLNQSEYLNNSFSQDKQINLQILRSNFGKLYSDLVNIDQNYNQPQYNITLLKNQITFNNQASFSIILRENTIALLSYSKETLEYQKSLLFFRVNIIQAYENTIYLIDEFTSILNDYSTNILVFWQTILIIQIILFIVPLIINIRNWYKFEKRHKYLVQIISRISEDQASKLVEIKQQQISYSETEKQQINVNHSYYTCKSPLRLMQSTKYQSYQNLRQRDTQLLYEKIQNKSFSITMKLGVALFFYIALVAFVSFGYFQIENSESHHRPIENLIKTYVKFEVQLGYLLSFAAILKGQHIYEEQLGKINDPEIGDFRNYFQDDKVPKYFQNISQSYQKKIISIFSSIILSDYIDENDKSELYDLYKGDFCDYLQDILPFCNITIPLNQFDMIYGWFYPYENNSEILRKGIIGFISNLDSLFKNDFEIEISQGIYQLNISNDIYYYKEYNNLVIQYFFNISQGFELFYDKIDIISQNLIIEKKQNILVYFYTFGLTFLLVYIIITVIQIIYAQRRYKNCILGLVTLTEDLLNDKTNLSLLKKLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SPT9</accession>
    <name>A0A8S1SPT9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0100394</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SPT9"/>
    <dbReference type="OrthoDB" id="299264at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="232"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="30"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8141960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="617" mass="74394" checksum="05C5673C298B9BB8" modified="2022-10-12" version="1">MSNEEKSQQNEQPQKRQRRPRQHTNEEESNQFWKNNQYFTQGQIDDDDDEDSEEYYPQQSSSVSEEISHENDACDYIDSLDQWQDCSDSKEQLKQQQESPSKSIIKLMRKNLEQFSTMRGLHTQVDIATLSRLGETSYVYYNEEQKFKNEFQDVLNRYYKSIPYSKQRRGDDDQIKCKTEFHGDLFDFEDKRYFIDSEIQQLQRVLKLYKTQKFDSYLAYRKLTRDQNLDFEQSIKKQLELLRQKKKQIEAQLEEEKRCFYLSIDQQYILKLKEYTKCLTPRYIQSQSNLKYDQMLQNENPSFQIYPYDKQPQWLLQYQETFNIFKKKFQQLQAPLYFYSFQDANALKITSHFILKSIKQLEEVSKNKLIAKIFIDLINALFNIDIPEADLLFNTELLNEEIINILTSQEKQILLDQPEQYKPFPTPDDPGEETKTKLKFYPNFKTEEYALYGRPVFEFSMRFLYEIYFRIQRITQISTKITYKNKEYVIGNLIMECCYKYLEDEISYAAWLTICKSIFNEKDSIEMQSLILLVQMFRRLFYQYDEWTIELLINWIYKILGDDSKILNDDVQISNVSIQDVLLKLKNNSELQNAKMTRIQTVDGLMIIHVFEFTQLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SRE6</accession>
    <name>A0A8S1SRE6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nephrocystin-4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0120181</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="313446at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097730">
        <property type="term" value="C:non-motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058686">
        <property type="entry name" value="Ig_NPHP4_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029775">
        <property type="entry name" value="NPHP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058765">
        <property type="entry name" value="NPHP4_C2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31043">
        <property type="entry name" value="NEPHROCYSTIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31043:SF3">
        <property type="entry name" value="NEPHROCYSTIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26015">
        <property type="entry name" value="Ig_NPH4_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26186">
        <property type="entry name" value="NPHP4_C2_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NPHP4 C2-like" evidence="3">
        <location>
            <begin position="694"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="domain" description="NPHP4 Ig-like" evidence="2">
        <location>
            <begin position="1130"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="485"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF26186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8143355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1309" mass="151930" checksum="5DF689B04F1D2CB0" modified="2022-10-12" version="1">MSTEDINSIKKFLKFQKMHKSLPSSIPIQMGLDSKAFKLIVSRIGGLRRPQNPVLNLQFSVAANFFIQNQQGEKCFFGRTYNSPRLNVEFGPDGVNYEHPIIPIYYHTRILNNKEMPLLIILEIQSYETQQDLVLKRMHLGWCELQALTITTQSTKLNFRKGSPRILLLGQQVNFESSGISVTCDLVQSEEFQISMHLSPENALCGLGDELPGLKKSFVGGDFELQRSMVVFMSKIQVLIPDTMEADIYRQVQKYRTYKYRTADDYKGDIQIINKRLIVAFHNGWTFINSRGTNNFVPLGETRMLVNEFVDQKQIQYKQLAYHGVIDINNVCSTDPTVNSGMLVAQLEYEAMFLVSNLTKETMQLTLGWIPIYINNKFGADELVEEDFIVGPGKTLDGRPLVSFQNPELSIKLKGSLNLSGGGFQIQDLYMGAASTQQQQFPQQQQGLQQQPNLNLNQPHQQQIQNKQEQQVRGSDIDIRTQVKMKDFEQQLLRLQEENERLRRLAIQQTSDPQKQQEYLQAIEKLREENRLLQFTQARQFEGLEKLIKDQKAVDRTAAPPVVLAEAKPQNTVIKPIDSYAYPVPSATLKPITRTDVTALVDDPISMDQENRLMALEMQDERKDQIIVIQPVSMKQILGPYPICKSVQIGIIFYDLQTFKSPFLTLEYYNDERNFKYNLENGNPLRLLYNNEVFSCKFNIETSLQRYANLEQDFARYLFNKNLILDIWDSESLFLFGSIRIPLRGIMRGGKQNAQIMADVEIQDPQSKKVKGTMQIVIKNIGKIGQQDMSKTVPLENTQNGFQKKKLVSEKPVNRMEVPAQAFDGNSIQDRIKKIKQSNLQEMIKNKQSQQRSLNEVLKYKEIKKPSLVRTIMQGFLQNEKVVHSFYGKCEIVPLYFKNTRPKPEQFTVNIEDPESRMLEYQEFFLIGNPAEWKYWVTNNKYPEPENYDLIRMKENQGKTQYYFILDPNQEVTLLFKFISFRQYDYRVDDHERTPEYIKKYMQSRHITILLQQDGKMMDGLRIIVEPHQQPIDHVLRFYERDNRQTRLVIPRLFNQPLQQQPHVHITDPDVMIEWSSDFNSIRLNLRIGEPQSVKIFNILLYSDQYQHKIIANWKIEVYSLSTIDIEVPLGQRVCTKITFPCDITRIIKMSSSNKMISFVKPFDSEITMLPGKINLVPINILALQDGKHLIKLNGVDIHSQELIYSWLVNVHAEPQPPSATYDLRCIDGRESRHQVNYKNQVNYTTQYNIVSSSEYVKIIDHSISTGPNEIVQFKIRVLPGSEELVRIFITDIDERIFDVIVLNIKYVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1STJ1</accession>
    <name>A0A8S1STJ1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0100326</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lin-54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1STJ1"/>
    <dbReference type="OrthoDB" id="6283463at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005172">
        <property type="entry name" value="CRC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028307">
        <property type="entry name" value="Lin-54_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446:SF34">
        <property type="entry name" value="PROTEIN LIN-54 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446">
        <property type="entry name" value="TESMIN/TSO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03638">
        <property type="entry name" value="TCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51634">
        <property type="entry name" value="CRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRC" evidence="4">
        <location>
            <begin position="191"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8141824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="332" mass="38695" checksum="F492A19AA1C1C648" modified="2022-10-12" version="1">MSSEFIQDGTPFTKLFQRLEKIHKSPVFNAECIHNQDEIYIKDQSPEQVCKKLEFTPCLKSPNQFQPYSPLNHQTTKKANIFIISPARQINFDHTKTEQRLVNISSPLSSLKVPNDLLCEEEEILSDDQDIHSQFRSPPKSPHRKLERATKLKFINFVTLKKPLKFTSDQVNNRKRTSSKRRKPLTEQKPKIIVCNCKKSKCLKLYCDCFAAGVPCGKDCNCCSCHNNDDHTKERENIIQQIMERNPQAFRPKVESRSNSEDEIDQKPRHFKGCNCKKSNCLKKYCECYQMGVKCSELCKCDDCKNCEMPIKKESRKRVKHTHSCFKNVEMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SUX8</accession>
    <name>A0A8S1SUX8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0100406</name>
        <name evidence="8" type="ORF">PPENT_87.1.T0130379</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SUX8"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000586">
        <property type="entry name" value="Rab family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="208"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="214"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8141984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8144745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="252" mass="28933" checksum="61FC9FA1FA401C5F" modified="2022-10-12" version="1">MSSTGNLNDKFDTSFVYLFKYIIIGDMAVGKSCLLMQFIDKRFRSKHDVTIGVEFGARIIRIQNVAIKLSIWDTAGQESFRSITRSYYRSSAAAIIVYDITKRNSFENVVKWLDDARENGNKQITFLLIGNKNDLEQERQVSFEEAKQFAQDNEIGFMETSAKTNYNVDQMFTKLAEIILFKIQKGIIDPKNESFGVRVGSEYQKYLASQSNDSKQEEKEKNQKIDPQLDGKYIQLNDPAVETTKQKNKSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SVF5</accession>
    <name>A0A8S1SVF5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rubicon Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0120093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SVF5"/>
    <dbReference type="OrthoDB" id="1918044at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901981">
        <property type="term" value="F:phosphatidylinositol phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052428">
        <property type="entry name" value="Autophagy_HostDef_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025258">
        <property type="entry name" value="RH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45971">
        <property type="entry name" value="PHOX (PX) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45971:SF1">
        <property type="entry name" value="RUBICON, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13901">
        <property type="entry name" value="RH_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01175">
        <property type="entry name" value="DUF4206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rubicon Homology" evidence="1">
        <location>
            <begin position="108"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8143207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="301" mass="35499" checksum="F9A3AAB4D4B839B1" modified="2022-10-12" version="1">MSWINQNQVTINKPNNLLGLFSQSRDSLHHDPSEQFTKLPPHLYTKHKGKKPSQILYDEIYKEIIETPNINRFVQPPPQYELKDQNFKCWQCQKPISNGFLTIGANCDQCFFHGRYFCNDCMSVVRMPIPWKALDSFDLRHYKVSKLAQSEIDKLYDLPILEITPTSKLLQLNKTLFEFLVLKRQIHLLYDMICDPKLVQTLLEKRMNLCLKRNCFSLKDLYEIYNGSLTKVIQGYYVILYKHIDLCKSCQKRGHICSICQHMVPIHAFDIKNVTYCDTCLKVYHRECAEQKSCPNCLQYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T000</accession>
    <name>A0A8S1T000_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0140364</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T000"/>
    <dbReference type="OrthoDB" id="298777at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8145673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="498" mass="59407" checksum="B49E2E6E27AC4EB6" modified="2022-10-12" version="1">MNNLVEQKSHLLDEKFEQYDDGTYEYDGILWDYNINPPRKIRTKFLVTFTESKQIYYTQDKICIRIYQYKNISDKHEVMNNLEQIYYLQLIGEYGKNNQKIGKWRATWDGEILKDVGGEYSEDGKKQGQWKEINKNFWREVNLQFYSLAQVYEVGQYVNDQRNGTWKFIYENNEIGGGKYNMDSQKNGKWIDLSSEFWSESQVTYSGEYKNGKKIGIWDVWFYDDNDKKKNQLIGCGLYQEKQGESIKIGRWYELSDEFCKESQIIYSGEYKNDKKVGRWDIFYRRKAKYQFKQIQFLVVEGHMMKNQKQQTQIRLDNGLNWAMGLRKILKQFLQVNIKMVKRQVSGIFNMKENICKIILLIQYDKKYSGGGQYDDKVEKVDSIKIGKWIELFDGFSNISQVIYNGEYINGKQVGRWDILYRKKNDIPFKMIGGGQYDENLYKVDSIKIGVWIELSETFYKYSQIIYNGEYKNGKKVGKWVKIDLMANEQIQEIDYDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T2J9</accession>
    <name>A0A8S1T2J9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0140404</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T2J9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8145736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="50" mass="5825" checksum="E9457D31DF22FE21" modified="2022-10-12" version="1">MGRSQMTNNGNEQNPPSIHIRNFKVENYRFKLKLSVIEIHVILLGSKKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T5T3</accession>
    <name>A0A8S1T5T3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0180174</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T5T3"/>
    <dbReference type="OrthoDB" id="287063at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8148655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="308" mass="35223" checksum="D08F28292A117E1E" modified="2022-10-12" version="1">MSQLTESPNRLNEQQSIPHDEIIDKSQKLATSSPDKENSQELFRLQKQQFLQKMTQDNSTRRQKKIIGDIKDVVNVNKQIVNFGLVIPGNICEDDIQFQNLSNETVIIGIQVICNNTEFDDLDEYVYSARKLNGYDYNDRFMLACPAQKQFSMKIALKVPNIKEQKGLFGTIIITASYSNHSKIQGQINTQIQSQITLPSIECPKTLMNSAYNLPVIQMAFKLGKKLDCKIPFRNNSLIGLPLEMEFLNKNDNEILINPPTLSVQGNSQFLVTIYIKSKQEQIIKNVLIVKVKNSNVHFSYPFIIKIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T688</accession>
    <name>A0A8S1T688_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0180240</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T688"/>
    <dbReference type="OrthoDB" id="288334at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8148785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="120" mass="13724" checksum="A5DF6C7FD40B5DFF" modified="2022-10-12" version="1">MFCCSGKKKQREQIVSTKNKSKILTEFSEEINDEAMTLYQSPYCQNISIMFQYPADPNSMRTGSNTKITRSVERLTNALPKIVPRKTSFNSFKYSKTNQNTKFVSTKKGFTLLVTNTNPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T961</accession>
    <name>A0A8S1T961_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0170087</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T961"/>
    <dbReference type="OrthoDB" id="297346at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8147684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="279" mass="33332" checksum="96C467A64D4C2EC5" modified="2022-10-12" version="1">MNPKKLPKIYRNDLENSFIRKFERSFISQSLKSSQVTRVRLYKQKQPRYQINENLKQLSQWNELKQDHKKLKYYPRGKSSESSIEGKNGKKVSQFFLLLPYHRPTQQRFNQCRRMWKCLILGVQIILFYSKLYREQLISKTASCPHIPPRRSSLKSLNYPTSPPKRKFRSRTQGEGKNQPNGFGDYVKAMYESEKQKKELCSKSVITKEFRFNDSRIKRLMTSKICDQTLDLSRNRCTSNKDTNQLPILMQIAPYKEENIRIKRVSSTMIRRIRTLRLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TAY8</accession>
    <name>A0A8S1TAY8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0170164</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TAY8"/>
    <dbReference type="OrthoDB" id="294928at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="126"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8147832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="395" mass="47323" checksum="B650107D17CE3838" modified="2022-10-12" version="1">MYELNVHKCAKFRTFENKADTRRALVFLKLLDNSCPREEALKFCKKRICRFDTTKKLEGPPKFRLIRNATTSRGWIFPEEIKYDLGKNYERPKTEEDRAFQKFFQSNKVKIVQEQQHQVEVVNKFMKEIDGQLNMKYNNLKELKKKVQDVRVKQEEYRNTPVNYLKFKGQRFIQLYDVFKKHYDNDLLGFEKQFPVTVQEKTKRNEDFFVRANKKLETDNLQKKFIKLQNEGEIIAKLTQSEGNKDQGISIKSFQHINDVKNLAMEFNKTQYVFDDEKLKKMKRNSLHLLKLNNKYRKNKNQKDTKIGDFEEKLERIITNRIDSITRSGNATNVDYDQINSLKQKTETARNKEFYKIKSRFTPRSSKMNQRAYTTQQSTRKLVLKSNRSHNQLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TB55</accession>
    <name>A0A8S1TB55_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0180281</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TB55"/>
    <dbReference type="OrthoDB" id="9332038at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14133">
        <property type="entry name" value="PKc_DYRK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF17">
        <property type="entry name" value="HOMEODOMAIN INTERACTING PROTEIN KINASE, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="100"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8148867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="433" mass="50624" checksum="9481833D95A9FEA5" modified="2022-10-12" version="1">MFNLTLSFSQFGIQKTHFQLYDCQNESLKTKQLQRDKQPIHLVTTKIRQLLQFTQTISVIPRTLTQPNAAVYNDGLDNENYDLIIRLDDVIINEKTGNQYIAKELLGVGTFGSVYRCVKNNSNFAIKIIKNINAYNIQAMKEIKLIRFLNKKQEHEYIIKLIDYFLFKNHVCIVFPLLGFNLEELLQETKYQGLSLHMIKNILKQLISGLEFLHSLNWVHCDIKPENILFTDNTAKQIQIIDFGSASELNVNLYYYIQSLYYRAPEIILGYKKTCAIDMWSVGCVAAQLYLGYPLFQGTSSFDQLSKILGLITITNQELIQNSPKFNDYFIKENQNYVLKHLKKYENEQTLNLHDPGIQNITNIQELYLEFNKSSLRTIQEMATMEDFVDLLKKLLEFDPVLRITASQCLQHPFLRDFQFNINDILKQELQYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TC54</accession>
    <name>A0A8S1TC54_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Parkin co-regulated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0190100</name>
        <name evidence="3" type="ORF">PPENT_87.1.T0190101</name>
        <name evidence="4" type="ORF">PPENT_87.1.T0190108</name>
        <name evidence="5" type="ORF">PPENT_87.1.T0190109</name>
        <name evidence="6" type="ORF">PPENT_87.1.T0230269</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TC54"/>
    <dbReference type="OrthoDB" id="300918at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019399">
        <property type="entry name" value="Parkin_co-regulated_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21207:SF2">
        <property type="entry name" value="PARKIN COREGULATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21207">
        <property type="entry name" value="PARKIN COREGULATED GENE PROTEIN PARK2 COREGULATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10274">
        <property type="entry name" value="ParcG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8149420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8149422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8149436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8149438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8152844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="256" mass="29205" checksum="00656D6BF5C052BE" modified="2022-10-12" version="1">MLQQQQTNKRIKGHSPQRSPGKFCSNLLNISNKFAGTGGMCSSLAPKKSQAPQKVPKSGIFQALPMIEQTLFKKYYDRGDLPIAVNFSGAVRKIVWKCEPECLDYHLYLPIFFEGLREVEDPYKFLAVNGCEELLQKGETKILSVLPQLIIPIKKALATKNHDIMCITLKKIQKLVKSGQMIGEALVPYYRQILPVMNMYKNKRLNIGDKIDYSQRKNENLSDLIQETLETLEKNGGEDAYINIKYMIPTYESCMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TFS7</accession>
    <name>A0A8S1TFS7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0200371</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TFS7"/>
    <dbReference type="OrthoDB" id="432483at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="305"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="353"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="383"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="6">
        <location>
            <begin position="520"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8150613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="880" mass="103993" checksum="7AE6CDF93EA285BE" modified="2022-10-12" version="1">MIQEESLPGCNNSNLYHSIHDQNQTVQQIQCEEFERIGDIPGQRENSIQSEVKKKQFSQLMVQFNIHHFKRKMLSYIHPYKELSKNQFSMISDKSSSFKSYPKKQQQKTSLLQHNRAVLGFRNIIKEFNNTLVAQTKKKIQYLIGIIPIINPYSTTKLIWDGIITIMRIYLLIWIPILIAFKNGTLEDMNAYTLNVCSSAFLIDLAMQTFTIRFDKGFPVKDRYQLIQYQINWWTAIELFSFIISLYFSIQFHQNNSSMLVVEDKGWTKILLLLLIVQTKNILQFIENLQSVIKPSKSTNSLIELVKLICLILLIQHIFSCIWVIIGTYEHLKAQSSWLDIAKLDIDQPWHDIYLEAMYFISVTTFTVGYGDITPQNISEKSFIIFYMFFCTLQLSYSVNTIGSILIQLKENNEEIKQKMTAITEHMRNRQMSRGLQFKVREYLTYYWQQENVQKKNETAEIIRLLPEELQKSIQREGSSNLINKCQFFKEKFTPGFLNQLVEIAYVQNFQPGIIINDLQNIYIIEYGYVEILQNQTVVGQLSQFQQFGLKKVSMDLINETKYRTKSFTNLLIIPYAALVQLVAQNQNELEIVSQTSFSIHNECIICKGSHKTELCSQVHFIPDNEKVIKKYLYNSTQNRGRIKRKPKDQFQEWKNELKFFQDTAEQFQQDCNNEIELLFPNNEKQQQINLCTQNSLLEINDEKPKSIILNMPIGQRGEFEQKVENKEAQKSDRKLEIKNEINNELNLIQQFESISQKFKQIQLYNEKVQKDIFLLYKQLECLLGQNECEIDKYKSYKHYYKIWNIERIIVDLNFASNYINRRRSEKVGLEQISKYLLFPKIYFQKYRTEKPSEILQQPLVNKSIRTSKKKKRIAIHPDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TI42</accession>
    <name>A0A8S1TI42_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0210301</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TI42"/>
    <dbReference type="OrthoDB" id="301479at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="257"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="315"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="504"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="539"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="163"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="317"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="335"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="507"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8151334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="653" mass="76764" checksum="F15C52FA0327EAB1" modified="2022-10-12" version="1">MSGSISSSDSEETSFVLTEQERILKSSNIFKQKEQSNNKCSKHPNKKAKYYVQCDKTKQFCSKCALTLALKGLKIEETQENQHEVLRQQRINRFQELLQQIMEQCTHKSQEFSNIETISSKQLLEHQESCQQFFDSVIQTANQLKQTYLQKFQNDHHMLLNSIQSKANQIKQIDAQIKQFQIDIEKNHNNIVKKMDMKPFEDIMSRYEKRVLQTKEQLQECTQEFQAKPIKFEQSQILADMNKMCYNLLLLKSEDSSTSNNHSLQIKLENSPKIRQNTNNSPVDMKVFEMLEAEDIYQSTYSNPIKDHTQSPIRILQQQQQSSQQQIQKSKSKSNTYSNPTSTIQNSRRESRTNTPESWQQTNYIQKNNQLITQNERESYKHNISVGEQLNSQKCLNKQLEMNEKYYVQKNEKQKTSSEFDKNNEQSNQQLINNLNQLYMQKHNKDTDRKSFEDGLQLTPKHQKNMTAAGPQTFKLLTNHASQRSQYQYPQVNNNLFEQKSQKEFSKTNYDSLPNQKSLTPLHQEPQSRNNQQLNQKIDNHQNRRSNSKQPKQPSFDQIEGKRQYIIANINSQQYTSQPSQYVQNEDTLKDRILKELCSHPGESIYNQVLKLNSQQKQKNQKLISKENVEQSTTVRNKNNNGFLCVKKQSYQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TPU4</accession>
    <name>A0A8S1TPU4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FHA domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0250166</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8154058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TPU4"/>
    <dbReference type="OrthoDB" id="5855668at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00060">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd09487">
        <property type="entry name" value="SAM_superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000141">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050923">
        <property type="entry name" value="Cell_Proc_Reg/RNA_Proc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23308">
        <property type="entry name" value="NUCLEAR INHIBITOR OF PROTEIN PHOSPHATASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="2">
        <location>
            <begin position="17"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="1">
        <location>
            <begin position="185"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8154058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="366" mass="42494" checksum="B128F2F54BD2FE89" modified="2022-10-12" version="1">MDYDDLYKVLPKDPKKWKLDDVTIWLKFIGLQDLDVNFRQNSVDGALLSTLDDNDLKEMEIAESSLKIKKLVQWIKIGFKEYSEYLKQKEKDQTFDSQKQIINQQYDENNASNNLVVSNQFKRSSIKAAIFEDQIQSNQSFKQTVQEVNNVQEIIKSTQMKQECSPYLFNINNGNQRILIPKEGISIGRNPDNTLVLKEEYVSRQHCKIFYKENNGSFYLQDLGSSSGTYVQLTNPTLLKEGLILHMGLGQFLISKIKIQGNKCSVFIRVLEGIMENKNLDFELTQDQTALIFGRQMSMFVDDTHLSGQHAQFTYIEEGLVIEDLDSRNGTWLRLSPPHKQSEPVKLIDGRRFRLAFEQFFEFHTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TQ42</accession>
    <name>A0A8S1TQ42_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0260161</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8154825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TQ42"/>
    <dbReference type="OrthoDB" id="305644at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="30"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="175"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="47"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="192"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8154825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="404" mass="46876" checksum="F791C74A44A88A3B" modified="2022-10-12" version="1">MAEQGRYSKLSEEEAAAQFELRKQQLLGNLQEQYQNANGQKNKKQSKSQQQQINQQEFIVFNPTKKQQSQPSKKPSKKLQFVKEELPIRNPTPPPEQEIDYEVQQPVEPIPIEQSPLIDKPQKPSIKKMLLRDIGNLDLEIQRLTRETKDFQNEKLLNKSQLSQQPRNLKESITKINSRPSSSQQQQSKAYFELRQRPSSKESRISDASYTKKAECFKGVIRSSSLKKLPQPPKTQYNFLQLRSASEKKINQSINKTNTIESENKDVSNKIVQSERKIPKPIIQINRSQSKQDTPTTYEKKQSTPVKSILKVQFNSIPVECNSTKNNSSSYFHQHKESYNSQISFSKNKCVEAQIEEAVQLYNNLKSLMQNKTTVLQIRKDGNLIPRKTADFFYHKEMQKPTLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TQ94</accession>
    <name>A0A8S1TQ94_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NlpC/P60 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0250149</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8154024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TQ94"/>
    <dbReference type="OrthoDB" id="202825at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000064">
        <property type="entry name" value="NLP_P60_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47664:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_14, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47664">
        <property type="entry name" value="NLPC_P60 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00877">
        <property type="entry name" value="NLPC_P60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51935">
        <property type="entry name" value="NLPC_P60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="NlpC/P60" evidence="4">
        <location>
            <begin position="37"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8154024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="624" mass="74482" checksum="2BA18C8C8575690D" modified="2022-10-12" version="1">MDRNLQSCFQQFQVKRNQNFLYQKYVNKTNKEKIPDEELRQKFIAQAYKYVGVPYHSRYHDETSPHFNAPLYLDCSGLIRQVIYDLREYFGFTLGKWNQAYQYDLLDKEIELQDMKPGDLIFLSGTYYDKSCRAFQHNIVHVEIYLGGERTIAARWQKGTVQEFETFKFESQNYYDIKYHFKSIQPWLEGRCESFCAEHPWRDDRNIWVSDKYSVFAKNLQQVLPADFVHKQRVYVGQGNNAELIKSYFRNKNYELMDTLQDAFKNDYFIKWVQCPQSINYYEFKPGQQIINHIPKASHNLGLKISMLELLRENQCEDVLPKTFRLNQITEVIEFLKDNEEGIWILKPYNLNCGRGIKFISDIARFKQDLHQKRQYQIGDYYKVYVENERNKLKQEIQDHLGIKENVDPEQQLKYDDINPMSIIQKYIEKPLLLDGRKFDIRCYALIAQVKPYTVLFHHGYARLSIFEYSLDDIENEQNKIIHLTNNAIQKTHPKYKEKKESSIWTMDHLEEYLQNFYHYQEDQIKSIREQIKQISYRIFKAGQKKIQAGGQKGEFELFGLDFIIDSNLKVYFLEANINPALFTENPNLKQIIPSVVEQTLDIVLAIQNNQQYQLGTFEELIIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TUC9</accession>
    <name>A0A8S1TUC9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0260341</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8155157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TUC9"/>
    <dbReference type="OrthoDB" id="293963at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8155157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="439" mass="51138" checksum="EA2E51CD33399715" modified="2022-10-12" version="1">MNNNICDYHPEQRITILCLYNQCKEDRLGCQKCLLSLHKSHIKSCVLISEIQKKQHCVELFANNQNEQQIVKFSEDELIHKDKLNSISNQLQQSLKRYLKQIKLNLLEQESKQKTIQMKHNKEILNSYFNSYEFEKLKEQLNKFKTGQSTEEQINDFISQIIKDRQNLQNVCQNQLTQGSKQNGLPQEFKDMVVTEVCNSLESIKNFMRKDDSIFFYKSQLSQEEDANIQETIKGKQFIFNDQDEVQLIYGSCTLSKGIYEFQIAIEYIQNEQSKQEEQQEGLASIVNNSPQLYNPYSQHTIGGHISNSGIFAQQEYQHLHRVYIGVVEDLVKDQMATYSIMYQTQQCCAVDIVRGAAIGSFHMKQQLGEKIEPIQTLFHKNMKKGLVLAFKINLDENKLILTDSENKTRYEGQLYNMTGDNIKPYILIYKSNVKVTIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TUI9</accession>
    <name>A0A8S1TUI9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">cholesterol 7-desaturase</fullName>
            <ecNumber evidence="9">1.14.19.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T0270165</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>cholesterol + NADH + O2 + H(+) = 7-dehydrocholesterol + NAD(+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:51644"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:17759"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.14.19.21"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:51645"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>cholesterol + NADPH + O2 + H(+) = 7-dehydrocholesterol + NADP(+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:45024"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:17759"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.19.21"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:45025"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Hormone biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Steroid hormone biosynthesis; dafachronic acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the cholesterol 7-desaturase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.19.21" evidence="9"/>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TUI9"/>
    <dbReference type="OrthoDB" id="283855at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0170056">
        <property type="term" value="F:cholesterol 7-desaturase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050584">
        <property type="entry name" value="Cholesterol_7-desaturase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045605">
        <property type="entry name" value="KshA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025982">
        <property type="entry name" value="SieB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21266:SF32">
        <property type="entry name" value="CHOLESTEROL 7-DESATURASE NVD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21266">
        <property type="entry name" value="IRON-SULFUR DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19298">
        <property type="entry name" value="KshA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14163">
        <property type="entry name" value="SieB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="447"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="Rieske" evidence="13">
        <location>
            <begin position="57"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00026095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8155544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="545" mass="63991" checksum="7F681ACC7A5B42E1" modified="2022-10-12" version="1">MEWVRLEIIVPAILIILYYLYYRKFRFYLIAEKAQPNVLRHKLARGTPPPSYPNGWYRLCRSAELKIGQVEEIKFAGRHIAYYRGTDKVVYAVAAYCPHMGANLGIGGQVKFKSCIECPFHGWAFDGKSGLCVNSEKLDPKIVSTYCYNNIEKMDPNKKGEYLQKIEEGEIKIKTYLVKELRGIVYIWLHCLEAKPWYEPVSEQSDHLQLRGESVNFVNCHIQEIPENGADARHFDYIHSSVNDYIPTWIVSFKWTMKSLPANDPDFFKKMEHSKEKVRKYQKNLFDQYLSNKELRQYMNVLCLDAYLVFFNKWKFNVIWATGFQMGPATVALYVLSDVFEALYKQAIQPVERFNLKVFHTLYTTSSLPYWASAYILGGELRQVIADVSLWNNKIFTEKVAYNLKGDADPLLLKWRQWDPENLKKVQMLQNGEYEFNKLKMLQQEQLFIVLIVLVIIAIIIAHLITKCQGREKEQQVKDRLRKKFIKNNIETLNDMNEEEALLLQETFKNTLENSKQGKIAKYDARMQKIQEIRQKTVKFILTHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TY52</accession>
    <name>A0A8S1TY52_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0290041</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TY52"/>
    <dbReference type="OrthoDB" id="10330043at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8156532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="183" mass="21602" checksum="C075335CB1F49BCA" modified="2022-10-12" version="1">MKNIINSFSPFINRNSNIRNENIEFKKQLFHFEPLNLNTQLINQIRIKVERTLSPISNQNKHALFLTKINVQKKALKKIKLKQNIDYKLLQELDFNAKLQYLQPLVLPSTNRSHKKDNNQSKRQQCNDILQFCNSQIETERKIQSKINIRLPKIHKSIDINQSRSSLNSWTMNSSSSLFQINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U1W4</accession>
    <name>A0A8S1U1W4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0310327</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U1W4"/>
    <dbReference type="OrthoDB" id="301378at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8158414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="394" mass="46289" checksum="947F06741A1ADDD4" modified="2022-10-12" version="1">MGTSQKFIHRMRIPNYQFSQTTQANDYRAILNQLNKLHQGKQLDQKQVSSLIQQLKPYLPQFENQEIRELVSICFQQQINDENYAQKVFDRTTQLYLEQQASLTETLDILSLLVSGNYSKQFFQQQFNFVAAVSQAPDLNASQIGLAMFIYGQVSQYTKSTNKEVEQSFILNFKQQIDHFKSGDLKHFSFGLILARIQRKDIFDLLEKQTLVTEMEFQDLIRVGTGIALFGNGSPEFWKLLEEQAIKNIPTTEPQNITTLFMLYKQFGHGTFEIFKLFEQQFIERYDQFNNLLKLQMFSSFAKIRYPSSAIFKLFFRDIVGIIQSVNITAVQMLIFDCQKIFPQFPKQVQQFFINFILKHYQKFNPVIKSKLYDSFQEQQLLTEELEVALLKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U2A8</accession>
    <name>A0A8S1U2A8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0320054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8158614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="308762at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8158614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="242" mass="28129" checksum="1027CA487A66DC78" modified="2022-10-12" version="1" fragment="single">MQFLVKSSGRVGFFGFATAAATHKDIAGKNFQKFQSKYAEYVTQFNQKLEAIEKENKAPNVAPQGKAFEHPYNNPHNPVNFSGIKSSELFYNFIGPEQVSPHYENFLVARKYLLLTYGGLIVIGFAAGTTNLHWIAKSSFLPFLFWMQIMYFYLEGRKSFMKPLLARFYRRVAGNECFQLDQYYHENMQLKIRTLLEAAKGQIEYGQLHKEFRDVKAELINTFLMNEQLNLQRHVVERSQXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U2X8</accession>
    <name>A0A8S1U2X8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosomal RNA methyltransferase FtsJ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0290291</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8157006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U2X8"/>
    <dbReference type="OrthoDB" id="20105at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016435">
        <property type="term" value="F:rRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008650">
        <property type="term" value="F:rRNA (uridine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01547">
        <property type="entry name" value="RNA_methyltr_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050082">
        <property type="entry name" value="RNA_methyltr_RlmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015507">
        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920:SF13">
        <property type="entry name" value="PRE-RRNA 2'-O-RIBOSE RNA METHYLTRANSFERASE FTSJ3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005461">
        <property type="entry name" value="23S_rRNA_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="5">
        <location>
            <begin position="40"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8157006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="244" mass="28623" checksum="7AE32884E9886F5D" modified="2022-10-12" version="1">MLKLKNLYSFSVSSQKKTKRWYMEHINDFYVKESKRLQLRSRAAFKLMQIDEKYKIYKSNQYVLDLGAAPGSWSQVVSEKCKVVAIDLLEVLPIQNVKFIQGDIMDKSIMKQIRNHKFDVVLSDMAPNVSGEHEADHQGITTLNQIALLLSTFTLKTDGNLVMKTFVGSEEKLNYDFFRLFFKEFYREKPLSSKQESSELFYIGKGFSSDLIKQILENTEKLYQYPEQIQSRLKNLIIDYKMFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U5X5</accession>
    <name>A0A8S1U5X5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0320320</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8159107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U5X5"/>
    <dbReference type="OrthoDB" id="286734at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030258">
        <property type="term" value="P:lipid modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049941">
        <property type="entry name" value="LPLAT_7/PORCN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906:SF4">
        <property type="entry name" value="LYSOPHOSPHOLIPID ACYLTRANSFERASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906">
        <property type="entry name" value="PORCUPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="98"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="337"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="377"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="412"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8159107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="438" mass="52399" checksum="733B74A0334F069F" modified="2022-10-12" version="1">MLSFDYLLKPFDQFDQLVMNSIKDLNISEDKVRLVSYFLFCIPIAIIFGNIKSPRIRLIYNIFWGVFFTLFLGREWFVYINITTILVYFISTIPTKNVVIPISITAFVALSYVHLFRQWFDYLSWKIDFSVIQMTVTCRLIYYAVDLQYGKAYDTGFWTYYSYVFSFFSIMIGPVPYHNYLNLLNKKEEYSNIKFNQYKSVITNLVKALLFCAGEIWIRPYVDYDWYDSPQWNELNLIEKNLLQAIIGLVARMRYFAGFKFTQAAMDATGITYNEDSQQFDKFITCDYSYEKDVSAIHKTTKWNTTVQIWLKYCFFDKLSIIFDVSTAYYMTYGISALWHGFYLGYYQTFFWWALINQNCKYVYRSRDKFQWIPAPLRSIIAFTYCTFTMNQIAMTMGLLDWDKGMRFNNSMYWSGPIAILIVFFFFFLTQYGQKRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U6F0</accession>
    <name>A0A8S1U6F0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Calcium-dependent protein kinase 1</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="ORF">PPENT_87.1.T0320224</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cell membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Parasitophorous vacuole membrane</location>
            <topology evidence="24">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="29">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8158919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U6F0"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020002">
        <property type="term" value="C:host cell plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020005">
        <property type="term" value="C:symbiont-containing vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000398">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000632">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000522">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 26" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17" id="KW-0969">Cilium</keyword>
    <keyword evidence="15" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1032">Host cell membrane</keyword>
    <keyword evidence="16" id="KW-1043">Host membrane</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0519">Myristate</keyword>
    <keyword evidence="12 26" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0564">Palmitate</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="27">
        <location>
            <begin position="62"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="360"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="402"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="438"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="475"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00060437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00068067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="EMBL" id="CAD8158919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="503" mass="58397" checksum="EC1C810DEF725C54" modified="2022-10-12" version="1">MGSYCCAQEVANSNETQIETRPIDFNSTDKKFSCHATKKITLLKVSPDLFIPMKRQDIYSVYSLGKALGEGAYGQVSTVTNKRTGMIRAMKAIRKDSLFEEEEQKLFQEMNILKDLDHPNIVKLCELFQDEKYYYLVTEYLQGGELFDRIQKAKTFSEKDAAHIMRQILSGVAYCHTKQIVHRDLKPENIVFTSKDEDAQLKIIDFGTSRRFESNKKMTKRLGTPYYIAPEVLLKQYNEKCDVWSCGVILFILLAGYPPFYGKKEVDIYQKIVKAHVSFHTAEWSRVSEPAKQLILKMLCKNVDQRISAQDALNDAWMIQHNQTNLVDQQFLKNLSQFSAKSKLKQSLLTFMACQMIQQNEVEDIQKKFKELDQNGDGTVSKEELIKAFQEKLLSKDYFVESLDEQMDKVIKQIDTNLSGKIDYTEFIIVCLQQQRMLTEEKIKQAFKILDMDGNNFISKDEFQQVMEGVDDILWNEFLEECDEDKDGKISEEEFIQIIMNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U702</accession>
    <name>A0A8S1U702_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0310328</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U702"/>
    <dbReference type="OrthoDB" id="303146at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047126">
        <property type="entry name" value="RNF141-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109">
        <property type="entry name" value="RING FINGER PROTEIN 141-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109:SF5">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="189"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="271"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="4">
        <location>
            <begin position="416"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="373"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="373"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8158416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="480" mass="56378" checksum="293C011BEC0357CF" modified="2022-10-12" version="1">MNQLQLSQAQSPRESNFDYEEQPQIQSIQLHQSRAALSIITNIQILVAIFQIFLILFNSYIAFDLSYKISLLYVTILHFLQIIKIGVYNNKKLYNNQYCCIQIFWGILSISYYGCLMYFLENNDFPIQYPILMHLIFTLTWFVLVLHNYFKNKNEKSFTLVLTVLFRFFLILQLMLINLKQIEWIDWQWIYIFTILWIFLALICIFQIIFLFDFICKATQLLQERDPINREAINQQIIGSLWINLLALCISGLPTFSMICYTIKLSTGQNSYNSLSITLSVIYSLVFIVYTMYYRISLSLFICQIQQQRGIDNNIGVNTDNQQRYQLNSPSSKHKNHLIKSKEQILTQLPQYLIRLSQTYFLPAIDNDNLPQLKQSQQQQIQQQQHSKKNKTDQEQPKLSNRISLTEINSDKDTQCFNCYQNESCAVYMPCGHGGLCVKCATEWFTEKQECLICRKPVESVVKISQSEQNKVQVIDVLAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U971</accession>
    <name>A0A8S1U971_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0350061</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U971"/>
    <dbReference type="OrthoDB" id="10263345at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990745">
        <property type="term" value="C:EARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040047">
        <property type="entry name" value="VPS50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13258">
        <property type="entry name" value="SYNDETIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13258:SF0">
        <property type="entry name" value="SYNDETIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8160587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="881" mass="106017" checksum="24022EABFA7858F5" modified="2022-10-12" version="1">MFNKKKDYIKKKYKDVPLLNIGDVNRILQQYKSDEDKFRMFTSILEIQKQHTTRTHESNTEEILRKNVQRLIEFYGDVDINHDALNNIYKNVDENQDIQTQIEKIEYSNIFQGDMNSQMISIQRQIERYDHYLNVIQTKLSKNVFTNYSSYLQALTHIDQIGNISQELLMKVRSSREKMQKTQQIVLKQSKQILEKRQSILNIQQLIETLKKIKDCYALPYERFVLSMQNGYIDASIYLPTIINIEIIDGLQLIPKNYKEDRIEYVRQLTCKGIMDQFINYDEKIYKIHYESYLYMEKNNIVQPYETIVHLLIFRAFEAKLQIVFDQISQILSIDFYFDNLTDSLNQISKDQLTEFYRELCCQTIHFFMNFHLIIRFHLDYSQNSSIIELRKLIYEKVFDRICDFINQTTSWPRMNKEDMICFFGMLTILISKCEVFGGQNLQKYKTFIEDKFSNYLESRSNDKQIKEQLINEDCKKCMKITLNQTQIVLQIANKQQKFHIFEYKDPFPDTLNIFKSIEYKKIIDLIMGSTVSFKKQTNISSLIYQGKERTLLLTQTINQIYIIIQQQLLYIHFFQQHKDKLIEELIFLINYYIYTLAIPMILDEHKRYIFDDTLNFNYDPKQEPDQYTQQFEHNSQVIIYREKYGDLIKYLKKIQIPEDLKVKKSQTFDIMDRVVYIESIIFVINEFDSIKEVFQEFKPFYSELNSIIGQLQELVIRDCINLELYAQLVQCKYELKQNDKDDQQRIQLIDKIISVNEIKLQGLSQHQLTEIQKLYYDELIYLFAVVYSRIKKVNNIGREVMLNDYYRVSKNFNIENSLYESYIKILNSNQADTIIEYMNNHQKQFPYKMLMGLFNAQGLQNCKKQQRKDQLFKIFQHYQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U986</accession>
    <name>A0A8S1U986_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0360310</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U986"/>
    <dbReference type="OrthoDB" id="311002at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8161685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="857" mass="102660" checksum="602E95B89E414770" modified="2022-10-12" version="1">MSRLLDIKYIYKNNYSILNKIYINEFITTFQCGQSGQRKLQFQIYSVQALKEQMKFQEILDFCEMTQKMFKEQFNQTISNYGIKKGTVNYYFIKNELDKELKYLIEQQSFALEKLDKFDQAIDLWDIAIKIDKKNQYNYEQKSIILERLGRYYQLIECWDQAVQFNKNNIHFYHQKALALNKMNRHQEVIQCWHQGVINNINNLQFYSQKSIALIKQNRIEEAFCCWDFGIQNNKTDAQYYIQKTLVLESQGKLQEVIECWDIGIKFNIHNEEFYVQKVMALEKQGKMNEIIDCWNLGIQNNKTFSYYYEQLSSFLEKIGKVGEMMNCWNLAIINNKNNLYFYQQNAQLLQKLGKIQEAIDLWDQGIKSNRNSGEFYQEKAILLEKQGQYLQIIDCWNQGIENNKKNQYFYQQKTIILERLGRYYQLIECWDQAVQFNKNNIHFYHQKALALNKMNRHQEVIQCWHQGVINNINNLQFYSQKSIALIKQNRIEEAFCCWDFGIQNNKTDAQYYIQKTLVLESQGKLQEVIECWDIGIKFNIHNEEFYVQKVMALEKQGKMNEIIDCWNLGIQNNKTFSYYYEQLSSFLEKIGKVGEMMNCWNLAIINNKNNLYFYQQNAQLLQKLGKIQEAIDLWDQGIKSNRNSGEFYQEKAILLEKQGQYLQIIDCWNQGIENNKKNQYFYQQKTNALEKVVDFESALRCWDEGIQNNKQKKFYYKQKANTLHKLGRIDELLQLWDLGIKNNVYDDYFYRKKANLLDQLGRSDEVLECWNIGIDNNKNQIIYYEQLQSKREMKDQELMQILNLGIKNNMSNITFYQLKVNSLQNQNKSDQVIECWQQGVNSSYSVFKKEFQFKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UB96</accession>
    <name>A0A8S1UB96_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0370210</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8162120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UB96"/>
    <dbReference type="OrthoDB" id="425067at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000457">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF5">
        <property type="entry name" value="MAJOR FACILITATOR-TYPE TRANSPORTER HXNZ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="182"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="304"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="374"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="397"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="430"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="461"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="58"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8162120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="491" mass="55644" checksum="FC757E4FF7384350" modified="2022-10-12" version="1">MNEIEQQEASHYSTINELKSEIQVVSQKEDHSIIKSETIINFDDTLTQIGFGRFQIMQYLVSIILGVMEGAQITIFTLMIPILKKEWDISESIVSLQTSFIFIGFLMGSMLSGQFTDRYGRKLPFIFSSLFTVLICFTTILCTNVYQLLIMRGFMGIFVGFFAPCCVTLLQEITPNNLRGQMTGSVTLSVAIGQLYGFFMASLILDGINGSWRLLTFLGSLPGLVALILSLLYIQESPRYLLLEGKYYDAFQTLKLMIKTNKTSEFNLSIENEHKLVIWSLYMNSIAKKTEHASFKSLLNGDRLQVSLVLWSIWFLLCFAYYGNLMTMPQILYQLKDNQSQLQQLVYACLSDILGAILATLIIDIKGFGRKNSLMIGFIVATAFSFLQLYYYEEYFAILAILQKLFLSMNYIFCYQLTTELYPTKLRTTGLGTAVAIGRLGVILMPWSCMIISQYSIIAPFLLFSIASFLGSIFTCFIPFDTLGKSLDNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UDW3</accession>
    <name>A0A8S1UDW3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mitochondrial cardiolipin hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0390048</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the phospholipase D family. MitoPLD/Zucchini subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UDW3"/>
    <dbReference type="OrthoDB" id="412219at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016891">
        <property type="term" value="F:RNA endonuclease activity producing 5'-phosphomonoesters, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051406">
        <property type="entry name" value="PLD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43856">
        <property type="entry name" value="CARDIOLIPIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43856:SF1">
        <property type="entry name" value="MITOCHONDRIAL CARDIOLIPIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="6">
        <location>
            <begin position="105"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8163105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="291" mass="34423" checksum="FC6AE1B63C6F4EB2" modified="2022-10-12" version="1">MDKIGVDQNKVKLNKEEIQHLFFPNKRNFHIYVEFLKQCQKKLIVCMYQISHKILVNILIDLSLNGRDIQIVTNSSNDDNKVKSTLLMMIQSSLEKIKIAVYEKQLCLMHSKYCVIDDQIIMTGSANWTNNAFRKNIESVVILKNEKEAQLYTCEFGKVWNQSQILRLKGQNLEFSPFINMEQMNFIKGKKQKCKFNKIIDLENSHLQDQDKDEEVKDEEVKEQSDESIQYYYSQKQKKMDNNSKRIKKNNQQSQRYNLQYNDFNEGNLIIQIDALDKQNADQIDEIEIML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UDZ5</accession>
    <name>A0A8S1UDZ5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0380125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UDZ5"/>
    <dbReference type="OrthoDB" id="75724at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46818:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_125, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46818">
        <property type="entry name" value="DOMAIN-CONTAINING PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="101"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8162564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="280" mass="33202" checksum="0B3E1D8C0AC568AA" modified="2022-10-12" version="1">MDQSYPLIKPNQEILNYEPTADEKKTKIGKYTIRRLFEGELDEQDNAAINKFLEEAGPFQNPIWTRPQILRFLQANNFKKDQTVQTMQQYEEWVKTLPINYDQQVEQFLKAGIIYIQGRDHSYRPIIVLNAYKVNFNEMSLEQYLRGLTYFLQVVVNDMMVPGKVENWVILIDLDYKGMIGLQINALKQVMSYLQNNYRSRLYRMFIFNTTMMLNVTWNMAKLFLEEITQQKIIFVKGDVKQLFQSVNKEQIEEKFGGTQTNRVEFWPPSIISANFRKNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UKW4</accession>
    <name>A0A8S1UKW4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0420250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UKW4"/>
    <dbReference type="OrthoDB" id="5337378at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053083">
        <property type="entry name" value="TF_kinase-domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44305:SF2">
        <property type="entry name" value="SI:DKEY-192D15.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44305">
        <property type="entry name" value="SI:DKEY-192D15.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="91"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8165510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="618" mass="73877" checksum="718A064DA7323990" modified="2022-10-12" version="1">MPFPTLQLTNKDCLNALNIQENELKSIIYKKGSTILLKNQKVKFFSQIHQDIIYNFYHCLQLNRIKKLIKLQSMSWTSLSNQSQFKDIYIILKDEYYGEGCFGQVYSCRKIDNTDPTIYCVKIIHIYQENQDIAQKSEIEDIIKTTISELDKNNPYQINLIQIFDIFYEGYFLYIVMEKCDEDLQIEFDQMIENNEWYTEEQVFDLIQQIITGYHSLHNYKIIHQNIKPENILVKYENKGQVNERKIYKISDFGIGRLIKELTKKNDLIRTGTSNYSAPQIFDNQIATEKSDIYSLSILFYQICHKSQLPYDCSSIQKRYQSFQNLKTNRFQAPPLPYNKGDILNNLIERMIVYREEERIQFQDIMHHSVMKARGIEDTVLFNNESTNFQSEKLYDKSQYLKKRRVTSILSRLKRLQRLLELFYNRFKLCHYFINKINNDENFQQLKLNVYLIAFYQLQYALALIYIRPFEMHPNTLQENDILILIDKLREVQNAIKNFSTEDVPKYKYLEKQIQEEYHKFNKQFQLFSNILHSKFINKTSHKDFLFFQEALMSKIKMQVLQSNITKLKNTLQGMLSKELQDLLDQIIKLEILFPVSIYKNDTDITLLFSINIQQTDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UTM9</accession>
    <name>A0A8S1UTM9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0490201</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UTM9"/>
    <dbReference type="OrthoDB" id="289147at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23107:SF0">
        <property type="entry name" value="IP09280P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23107">
        <property type="entry name" value="SYNOVIAL SARCOMA ASSOCIATED SS18 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="197"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="262"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="299"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="325"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="348"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="476"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="476"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="512"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8168608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="527" mass="62827" checksum="BD1A6F143FE1FB8E" modified="2022-10-12" version="1">MELEKQLYRIHERILSGLLIRKIFNAGSYLFPILTILGLVFLLLLQFREQYNQFLDQENHEHFNNESIYIFNITDTELKEKNESYLSMKFTINKTFLYMSDEFQQKYNFTVVTHFIDVDFYMKGFNTILCLATDLETMFIIDFLDFYQENDIQLMNQHTNETWSWNVQQFESNNVVAYDERIYTTVIQFIKCVLGTFLQSIVASIYMKMSIICAPILIIYMVSCMQICQNEDIQAQALVGAFPWVGQYLTILNRNHKLKQELLNAFIQMLILFYLVYFFQFSGYSGSIQLFAKSYPRGLSENFFSSFLLNEFVSIIFLRTRSSLYFVPKYITLTYLLFIYYFESTIYGYYNLAFQICIFSQFAIISIFVLHFEIAALEWSTISPYTPSFDRPRVLYCPMFNMNWVNDIPTLWTMFFPLCGRRFFQIQNLALVDKNYILLNNLLNQEEPIAIEDNAPAQVPNIQVQLELPQQQEQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQEQQQQQQQDNILGNDQPQQQNQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UTS8</accession>
    <name>A0A8S1UTS8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0480110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000048">
        <property type="protein sequence ID" value="CAD8167945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UTS8"/>
    <dbReference type="OrthoDB" id="288911at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8167945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="359" mass="42593" checksum="4752A177211FB588" modified="2022-10-12" version="1">MSLSQLSGKQGINYAKLRHKKSPNDQQSFGLFEDMNRNNLQYIESLIQKRKPSVDIKHQRAKSTLDNKQHSYRHGTPINDIEEFEGEKLFDQFFKLNNSKSDSDFMKESIQRDSVNKNNESDFLKFVENAIEYHKHSDVRFFRHSVNKQSHLEQKLKEITLSNRLLASQLNQYKQKEIDLKSKIKMMQKEFTIQYQKLVQQNSWLEELLAKQKIDNQNSLLAVKKTVEMLQIKCKCQKGNQLCDKILRDVKNQERVRQQYDWEDESKPTNSSSELSNNTQEEEEDSTDFNYQLKNRRKSISKVCHNSIQSSEEQQEQLVQSFLNGKSKNLREFPKDMFLRKKQTPDPYYIEEENTINSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UYM4</accession>
    <name>A0A8S1UYM4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0500262</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8169293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UYM4"/>
    <dbReference type="OrthoDB" id="2019833at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="644"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="domain" description="SPRY" evidence="3">
        <location>
            <begin position="656"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="55"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="435"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="491"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8169293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="767" mass="90672" checksum="02154A8818182D81" modified="2022-10-12" version="1">MQSELDKKLCQKHKLEILTIDLKQSTADEDKYLCIKCLMEKIDIQNMALVEETKIMIKQMKSEQLNSKIKEYQRKIENFRQIQTQVKELRFSINNTLDKVQCNLDQKITLMEKELEESESKTQITSFEEDIKVLSKNYKGSFSFEVPKEFEKSMDNNSYIDSVQQQLESIIKCPIFNQINESLESTKVEEDHKEIKQIQSLSKKEENPLKTPSLNIQCNKHGKEIIMLNLNPEKTKLSRQACVECIQSNDPIKYTTLSDANFKWNEYLGQTNDQIKQFKDLRCFKQRQIIETLEEIKEKYNSSISDIINRVNTQYSMFSQHESNEFNENMIYQMNAEQINELSEILSQTDKFQALTEKQFNIQKEDFNQMEIISTNFQKLLQNDIFAHNKIDKIINEQNSYIAEIKGQKSEIFQEENHISNQQSIQQLNLQLHHLQIYQDILNEAQNQYEFLMKTISNLSIEFKDLQLQQFNQYVSKIEKDFSIMKKLTNFDKVEAELKQNQQDKLQLQNQLTESEKTNLQNQQTIIELKQKDEEQIQIIQDLKNEKEVLRIKVKELEQQKSEFLVNLKEKQGQLERFQKSNEKNEEIVKQLQQTLEQQFSQQLTFSTTYKHNNCQVTQNGKVIDNNSGSWYCCMCDQMIPKNGIILFAFKIIEITYIMIGIGFRDIMQSKGYYNCYSIGGGTYNIYSSGHCYNHDQQDKNNKQIAFEFSTNDIIIVEVDIQKKYIKWTKQSTNQSFTLTIDTSKDLYPCVHLYNKCKIEILNQLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V2P7</accession>
    <name>A0A8S1V2P7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0540170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8171201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V2P7"/>
    <dbReference type="OrthoDB" id="307697at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="273"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="408"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8171201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="458" mass="54239" checksum="C169A7D99A4211F0" modified="2022-10-12" version="1">MSEFQQLTQQKVSIEQNQEMLQNWISLKQNLRSKFKSSQDAFKTLKKEGSLILTIEDFQEYSKGLDLSMLFKDVELNEGNFGRIWENWEYKQKQNEHKLQIINEKLKLIQMLENKEQNNETKADVTSNKSIQRLISNCESLDQLQEKLDMLVEEGQKQKNQQDIVQNETDCFSIYLSKKNEQQAEELYISKLQLKSNEDVEGHNQIVKKNIKSYIESQPHHRNKTELSSGTLLQSTLLNKGSLSNIPKSYKQSYITEPLLQNSPQKECITVRAQPKQNQQQINKSPYDERQQEERKSHKINRMKGDLQSYISQLFFHEREFRQNNIIYHKPSKQDQKGLAGFQNFRQNLKNQQEKLQFVELEDSNTSKRFQDIRYKLDDFQQHKLSQYNYITKTQPNFSPEKTDRTSNSKPISNQTNKANTKYSPGNIHRLNLEFLKQEKEQNNQIIQDKQIESQILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V481</accession>
    <name>A0A8S1V481_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0550183</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V481"/>
    <dbReference type="OrthoDB" id="303936at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1160"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="160"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="426"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="809"/>
            <end position="1112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8171741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1198" mass="143473" checksum="7D2F28B36D72A993" modified="2022-10-12" version="1">MNFDKSLKNRPTLSFTGFSPSKSNINIEASRKNLQSSSFGSPHHAQSLEAQLKQEFEAQYNERILTMRSFYDMKYQTLFETVKEALDKIMNDELIDTMRQDITSQEFVYQRVREMFEEIILSEREVLIEKLSSQYAYLKMEFGKIEQDKRKLAIELQIIQDEQADKEAQAQNQVQQAQEQISYLQQNNDQLNNKVSQLEQKLSSANQTAKEVQQLQTTAKEYNILKEEYEQLVQTNEKLQLINKQQVQEIEQLHKISENLQLKVQQLNKIYQELEGEVISYKKKSSDSESKTQFTISNLQNQLEAVTLQFTQQKSEYEKRLEKEAQTYQNEIHKLSQKLQKKKEKSTFHKQNAKQLQIRLDQLIRDHAEQLQNETQQFQRKINELQQQNQFQLQQAQKSLDDLKESHDKNIYNLRNQFEQINKQQCDDSDKQKINLLNELDRVQSELESVVLQQQQQIESNYIPKIQYEQQVNELQKKIIETNQKLKEQDALMKKQQDEFENYKEQWNRQQNKLHLDVKDRNEIQEQLLAREETINNQNKQISQLTQQVKMLESDYQRLQERSKQQLEYIEELKNDVSNRQSEVLKIQKQLKQQKDELMEQYRQLEGENKSKNDKVQLLNSQLERLLNESSENEKLLNYKSKELQQLEDELMQYQNFKRNAQQLSEENEQLRIQIDKLINIQELQEQKQEEIKRQLDTEQKQKNQLILNNESLMQRHQKYVKTIKEQRKYIKRRIVSELQKIKQEHASLEQYLRQIMRMKNNEDNLLIASIVTKVKDLWNRKEIQHENEILKIRNEIFDQYSGSASHLRTAYEQTLDETQKQFEQQIENLRDAKEKMEDENKELLHELQKVQVILEDQSALITNLKNENKTLKSNGMLIENELSTTLKNMNDMKDSFEDQIQELTKQLKKYERKQQHQQDLKGQIDILQNELEKLRTLNRSNTQETENRIAKLVKQQQSEVQLLINQFQREQIKLRQEQTDLKDQLSQNEEIIHKLYQSLDDVNSIVNQKDKQLQNLIEDIEQERASDQQKITDLQNKLRREEDEMKVTRQKGQRDIEKIQLQIASAESEISQFKIENKQLRIQCESLAEELEKKTKQFDQLNREYEDQKIIQNQEIADLHKLLKIQVQNDDMSNYYSESKELALRVKELQKINSQFSDTNYPRRQESSVQKFDSSKKLNSSNNLKPRLANYTPMTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V6T4</accession>
    <name>A0A8S1V6T4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0590047</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000059">
        <property type="protein sequence ID" value="CAD8173308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V6T4"/>
    <dbReference type="OrthoDB" id="313072at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="307"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8173308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="398" mass="47357" checksum="CF0D7701233CACAB" modified="2022-10-12" version="1">MIVSEQENIQIGILYDEKIIGITVKRYDMIKQYLCKYFSLPEKETAVKINEKICDSNENFDDYLKDDKLLEIHLFNINKLIDDQKELQTIKNSQIFNRLSIIENKSQVACQTIEQFNQFDNINQVDQNERVHNEVCSQISYQNQFSSQIIDKIVTCIHCLQQIDKDMIQTPCQHFFHFECLDQYIQNTLQLSFTILQCLCRCKLSLKIINMLGEQDKCKKYTELFLENQLFQIQKTYSQNIGRCIDNQDCKFWFFLQTNTNSQIHKKISCFQCSNQIDIKQTINSAPNQQISKIFQQVQLVKSYTQSNHIEDKIQDQEQEKEEEKEQVQKKFEQQDISNDIETQIVIESNKSCHINFQITDDKKVKEEQPNNNLQKQNEQEIIQDQEEKNLEQQIYNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V7P7</accession>
    <name>A0A8S1V7P7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">E3 ubiquitin-protein ligase CHFR</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Checkpoint with forkhead and RING finger domains protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">RING-type E3 ubiquitin transferase CHFR</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPENT_87.1.T0590068</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">PML body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CHFR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000059">
        <property type="protein sequence ID" value="CAD8173350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V7P7"/>
    <dbReference type="OrthoDB" id="1305878at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00060">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16503">
        <property type="entry name" value="RING-HC_CHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040909">
        <property type="entry name" value="CHFR_Znf-CRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052256">
        <property type="entry name" value="E3_ubiquitin-ligase_CHFR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16079:SF4">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16079">
        <property type="entry name" value="UBIQUITIN LIGASE PROTEIN CHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17979">
        <property type="entry name" value="zf-CRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FHA" evidence="15">
        <location>
            <begin position="23"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="179"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="299"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="299"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8173350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="601" mass="70362" checksum="0A923D5200320FAA" modified="2022-10-12" version="1">MFAKLLSLNTNTSSSIEISKPIFSIGRDQKNDKVFSDIKVSANHLTIEYKDSQFTVTDYSSNGTYINENKIGKGNTVTILNGDKIHLLPIQKVKDHEVIGFEFVVCINTEQQASKLSIESIDRQVSDGKREVKNENILNTKLVENQVESQIINEPSKQKKEQSPPPVPKIDGLEDELQCTICNDYLFEAVAANPCNHHFCGSCLSNWFKKQTYECPNCRSKLTGVMQARTINNLVEKWLKINPHEKRTEQLLNKMKEENLIYKNPQEYINLSAKFNQDQQQKKIQLNRNFEDEQDIYSDEENDEENQEENDENDSNDDDEYLSNDDNFDDNKQNQQIQQQQQYIPLQFQNNGFQGFNNLFPQNNLNGLFPQNHFQLAHGQNLFQQQFIQQNKVCKSCNGKIWKNFQCPPQQQHVGCASCARLMPKLDPSDDDNEVLQMQCCICKAYHCTFYYGDCNNAALQKFMLVKNIQHQMVIPYNYVNSTEYNRLLKHLGGRPQTVIFNFMMDNYISKGYFYFQQNKLTFNNPIQEINVKISQNSPICWLCHRKLINFIIFRYVQCMKYDEPLFMREDCYYGINCRLQQQNQVHAQKYNHFCEQTKFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V8K0</accession>
    <name>A0A8S1V8K0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0580243</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAPS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000058">
        <property type="protein sequence ID" value="CAD8173157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V8K0"/>
    <dbReference type="OrthoDB" id="299011at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007587">
        <property type="entry name" value="SAPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634:SF8">
        <property type="entry name" value="FIERY MOUNTAIN, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634">
        <property type="entry name" value="SIT4 YEAST -ASSOCIATING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04499">
        <property type="entry name" value="SAPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="735"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="795"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="126"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="735"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="746"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="803"/>
            <end position="814"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8173157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="854" mass="100027" checksum="160CADA859060464" modified="2022-10-12" version="1">MYKKHSTEKLVRKNASPTHEKENNSYARKYSRKLLSRQSNNDEYENVKENILTTYNSNTNTINQSESVTQLQQIIQQQKRQIQNLKMGCPRCLTKQNTDLELRNKILVQNNQILSLLSRETQQSDYTELFKINQMLQQQNEELKQNNEILIQNQQEILQENLILKNLFAETSYNVNAKENSSCNFIQLSLIELFSLSQSQGRFSSILQLNSQVQSPSHAKQHHEKFFARFFPQQQQLDSLLADPDCELETILSADNILQEIKGMGANKFADHIIKHPEMYQRMINYIISVDDELIDKKTQIQYPFLCSEILGSQNQKLLDFLFERPEEGIEADAEMIRVSLYDQLISFLEEDVFNMTSAGYFSKAILAIIKKRGFDVWTQIINNKQILSNLIKHIDCKHIAEIIEKLIILDTTQEFTEETYLNERKTLLQRIIILMQNKYYCSEIVENICDILIEITTKNMSCLYYNNPDIVPFIDQITRPELLFEISIKTQKQQPLQVLINLVEMSSKESKKEEDDEVIHNNEKDFSIFQNIVPELPQNLLSKRYCAANFYNSNQEQVQPFGLHKIMLLKLIQTFVQTNDITVIEALLNAGLIQVLETICIQYPSNNQIHIITEKIIKSILDLNDEKLTENVFEFGQLIEFIIGYHTEQNDKKGYLALLTSLSNYLFEKSYNQNILQYYKDNQQWMNFVKNRLFQINQKEKPFLCNVNPKAKSEVADDQESDIMSILQKFNDSQYGQQNQGEQQEQDKETQKNEQELETNNQQDDGGLEDDENIMMDMNVDQVWIEDILQTKKENQDDEAQGNLNQKSLQSTSDNDKEVIHKDYNISSLFNHSLFWKIEYDDKETEDILNNYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V9B7</accession>
    <name>A0A8S1V9B7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0590130</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000059">
        <property type="protein sequence ID" value="CAD8173447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V9B7"/>
    <dbReference type="OrthoDB" id="294404at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098554">
        <property type="term" value="C:cytoplasmic side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098553">
        <property type="term" value="C:lumenal side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042500">
        <property type="term" value="F:aspartic endopeptidase activity, intramembrane cleaving"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033619">
        <property type="term" value="P:membrane protein proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007369">
        <property type="entry name" value="Peptidase_A22B_SPP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174:SF23">
        <property type="entry name" value="MINOR HISTOCOMPATIBILITY ANTIGEN H13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174">
        <property type="entry name" value="SIGNAL PEPTIDE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04258">
        <property type="entry name" value="Peptidase_A22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="25"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="63"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="85"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="164"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="221"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8173447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="336" mass="39522" checksum="46A40E4CC253C292" modified="2022-10-12" version="1">MALLLIALFTSMSYKEKAEKIIKILVYIICVFTSFHFFNEIFGKFRNTLTFLAEKIYCLKVRDYLSIILSTLLIFFYLYYEQPWFVSNILSICILGSLIKLFKITSLKNALQFFVPLMIVDIFCSIYLSQTVRYEWDSVALRYFNTPLSAQFPYFRYIYKKKCAWVSIFNLLFPGFFLGYVNRFDKHKQTYVYEIIAFFGLLVGLIFWVLIQYIFSFPLPTSIFTEVLMILTTALVAVQRNEFNIFYTGNFYDQILMDPFKNDIALQEPTTLEMFGLGSTTQVIKKDTTQQQDLVSRGDQGLIFNSQLFVGLASINRPQQQQNVLQSKSQYQYHNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VEZ3</accession>
    <name>A0A8S1VEZ3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dynein light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0620223</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8175201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VEZ3"/>
    <dbReference type="OrthoDB" id="290485at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21450">
        <property type="entry name" value="DLC-like_DYNLL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001372">
        <property type="entry name" value="Dynein_light_chain_typ-1/2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01221">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01375">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8175201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="20424" checksum="F67450F19EB48400" modified="2022-10-12" version="1">MFQIVKADSGIDAKLEFEISNIVKGAYERFNNQYDRSKYISDYLDERYGGCWRVTIGKSFTSCGTYYLSQLLRLSYQNDQIEIVRTQGDSEFEIIQKDLGMNQAVFDSILGIIQNAQQTQKNLSAQVEYISDCVEQKHTGKWAVICGYDFNSRVPYVNNNLVCVAKKGIRYTVLMISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VF49</accession>
    <name>A0A8S1VF49_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0610145</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VF49"/>
    <dbReference type="OrthoDB" id="292564at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8174519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="528" mass="61926" checksum="9B549B9EE07A252B" modified="2022-10-12" version="1">MGCVSSDENKALAFDEFQFLKTGTLSIELTQLIFVVNNLIKEVHQLYKQVDSDRIILFRITKVSLTANQTLIDAFGFWTRFVSICNLGQGKLNGVYYKLEEDGRSLSYNLGLNGDLKLQWFGEVLNDYFKAIIDITVCIPLILEQLKFLQDKINHLGSNLKEKNQLFKYNMLLLKFNVAFTKIANIRIIKYNEEIEIFQQKYLQIADTADEQGLKLYQKFGAKQLNDRQKYRMKVKINIIMEQCFHGEMRSEIEQDLQNKQKEKRKALKPAWTFGDDIGIGVKKFPFRVRWTGCNCVDHSFFIISNYLENHSKHLLLIRKLSVVLRYLTQSYKTEEDTLSRAWFIFCTHLTPKERTIIQSNSSILNGLKKFKLKNPQAEKCRQYFIEYISLLDQDYLSQLQQQWQNYMDGQSKVLSLWSNNQIFSTDEFKALKVNDKYYALTSMSKNVYALQSYYPIATQIYNSVYQKKLQKGQAFLNQLSHIYNSSGYNPLPFDNLYTTNTTEDDEAKVKTKEIKFKYAFTKFAQNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VFR3</accession>
    <name>A0A8S1VFR3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0610246</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VFR3"/>
    <dbReference type="OrthoDB" id="9973183at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBC core" evidence="1">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8174719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="152" mass="17976" checksum="E6B6AEF14E8779FF" modified="2022-10-12" version="1">MALKCIQAQLEQLQRFSIEDPFINIQGGPIDDKDLFIWQGYIFGHKNSPYEDGMFQLQINFSKNYPFKPPKVKFITPIYHPNIDKDGNIQIDILYNNNWSPTITITQILLCISALLKDPDPDRFVPTIRESYQNEKYHYQTTEREWTQKFAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VJS1</accession>
    <name>A0A8S1VJS1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FPL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0670240</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000067">
        <property type="protein sequence ID" value="CAD8177500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VJS1"/>
    <dbReference type="OrthoDB" id="294052at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901096">
        <property type="term" value="P:regulation of autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039272">
        <property type="entry name" value="CLEC16A/TT9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019155">
        <property type="entry name" value="CLEC16A/TT9_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21481">
        <property type="entry name" value="PROTEIN CLEC16A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21481:SF0">
        <property type="entry name" value="PROTEIN CLEC16A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09758">
        <property type="entry name" value="FPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0072">Autophagy</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FPL" evidence="3">
        <location>
            <begin position="27"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="433"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8177500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="651" mass="77516" checksum="2A211F342876E80E" modified="2022-10-12" version="1">MQVIQDVKKLHKELQKPEKSHKYKQIIEELSEYFLFGDHQKEEIFDYFAEQNILNLFYQKLKSANHQLTIFIIERLSMIITNLQNPLNLNYVLSNPVLHEFINYNYDFNNPEIVDYYVNFLKTIAIRINRDNFYLYFNQRYCTFPLLWQAQKFINYPDELVKNTIQNIILSLSKLSSDPKSGKQTESVIFNQTKIMVQFKLYLTTSPFIQVYIKYIFQIQQMLESLNIDSQDQLDKLEDILMFFNDLITECPFLSTLLEKLILDKLILPILDSLLLNRKTSFILGYEVGLFTIYLFLTKLPLVYPIMSYFCEDQIFIDETIQNIKLVKQSQQWIYDTKNMEQEFQNIFQNELDDNQKDQYIGNKKSNLIQNPYKNQFLELLRSKHNTLLYLYLSIWFVAKQNNYQIPSMQIINIINLKEEIVFSKKLLELIILLMINEDLKELKQLYQDLKNKLVSIISNLKVTNKRLSEGLFVNWDIIENFDWDNFTNSQPVVKMEDLKPYVDNSKLSEFKYVYLWILVKCLVKDFKKKGVENQYQINQEIKIYQACELIQLANSQNYLMIDVNQGYLIHTQIQQDSESGIVKFVQPLKAIECSFMGDILILECNKLCINQFKPYQIKIQQDVITSVIDRIYQAQQSMSQLIINKLEALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VQ31</accession>
    <name>A0A8S1VQ31_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">cGMP-dependent protein kinase</fullName>
            <ecNumber evidence="6">2.7.11.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">PPENT_87.1.T0710095</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.12" evidence="6"/>
    <dbReference type="EMBL" id="CAJJDO010000071">
        <property type="protein sequence ID" value="CAD8179137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VQ31"/>
    <dbReference type="OrthoDB" id="290187at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030553">
        <property type="term" value="F:cGMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004692">
        <property type="term" value="F:cGMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd05572">
        <property type="entry name" value="STKc_cGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000068">
        <property type="entry name" value="cGMP-dependent protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000089">
        <property type="entry name" value="cGMP-dependent protein kinase 5-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035014">
        <property type="entry name" value="STKc_cGK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF37">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT PRKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0140">cGMP</keyword>
    <keyword evidence="15" id="KW-0142">cGMP-binding</keyword>
    <keyword evidence="21" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="23">
        <location>
            <begin position="98"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="23">
        <location>
            <begin position="216"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="23">
        <location>
            <begin position="339"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="469"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="24">
        <location>
            <begin position="729"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="21">
        <location>
            <begin position="193"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="498"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00024113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="CAD8179137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="815" mass="94083" checksum="9E0DB3D992548374" modified="2022-10-12" version="1">MDSKNQWNQKSSVQEFGIQKSSLAKPMHQQVKPAQQVLPKNEQDQNQLVRRVTKRNIAIKKSIATDEGTIYETFEPLMKPQTPQDIGFIVKCFQSHFVFSSMNETQLIQLAKSMFYCRLVIGQTIIKQGDGASSFFVLEKGKINVLVDGISRKQLSQGNGFGELALLYNAPRSATCVAMEECFLWGIDRHTFRKSVENVMRSEQEKNRQLLEQVKFFNQLTKDQKDAIAGVLILQKFNQNEVIVNEGDQASSFYIIVEGKCGVFNKEDVQITTLNPKDSFGESALKHDNQIRMMTIKAIEKDTKVVALGKDMITQILGDKVQYIIYKNICKWALNRSNLFGKVPGSIQDKLLEGVIYRKYVPGQKIINKGDKVGYLYIALEGNVLDEQKKIITNNILNEESLYDEVANTKHQQSFVMEMEGHVAVIDYDKFRNTYGSVEKLSENDQNNIYSKEFQQSNDLVKQFQLKELIFLNKLGSGQFGSVYLCKNKNLDTLFALKYVTRAHIKQYGIQKHVQQEKAVLEMMNHQFILKFYRSFKDGENIYFLTEYIPGEELFDAIREIGLLGKYDSQFYTAQMILQMEYLHTVHQIVYRDIKPENIMVDQFGYLKLIDMGTAKSLKNVSPPKTFTIIGTPHYMAPEVISGKGYGYFSDLWSVGCCLYEFLCGGLPFGEDQDDPFEIYKEIVKNPIHYPQYITDKTAKTIIEQLMNKIPECRLGGSYSSLKNNQWFSDFDWNKLLQRQLQPPLIPKKEKFMNESQIEEQLKKNILVIDQIQKDTMGSQKVFAQPKDTEWDSTTYYNNYFIQMILYQNIQLSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VSC4</accession>
    <name>A0A8S1VSC4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Golgi apparatus membrane protein TVP23 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0720139</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TVP23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000072">
        <property type="protein sequence ID" value="CAD8179631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VSC4"/>
    <dbReference type="OrthoDB" id="2151161at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008564">
        <property type="entry name" value="TVP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13019">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13019:SF7">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05832">
        <property type="entry name" value="DUF846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8179631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="224" mass="26054" checksum="BBA0C8E2B3CD20B0" modified="2022-10-12" version="1">MESNSLDFKINIQPKMLTEHVEPDVDLTVDKYDAVYQNTQQEEEVETAHKDLLIQEYDITEAAHPFYCVAALALTSIPGLSFILLQDVIHAYPLVIILQLAQFLLLKNYLGLKLIGLRWWIEMNINGEQKWMFQTQSQEQSNKVDRYFFWACLIYGSIFWGIMCLGDVFGFKIFWLPLPIICFVLSLTNLQGFYKCRGEHKKKLQQLKREMAKGGMNIVGMIMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VSY8</accession>
    <name>A0A8S1VSY8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0680017</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000068">
        <property type="protein sequence ID" value="CAD8177656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="298975at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1464"/>
            <end position="1486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1592"/>
            <end position="1617"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1815"/>
            <end position="1835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="957"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1163"/>
            <end position="1189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="969"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1167"/>
            <end position="1177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8177656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1874" mass="220522" checksum="1C59643DD9CDA9C3" modified="2022-10-12" version="1">MNWKILGDFISLIIQTNLSPSLNNQFFTSFQITFYYLQLTYFLVSENHEYIYRLINMLSRISLITPIFIDSYSINMLIGIIIILINIVPYIIITYRKITNKNNNISKSILQMSTMIVNYYFLYFSWFLYLPQLYYVGWNYIYSSASLLILASIVLILTILSLIISNVYFINFEFNEQHLRKHFSYSDLLAQLLMIPMALLYLNNDTTTQLISRILHGLILIIQIYDAYFSLPLGFSASGFILNRALMTHSVIFTFSSIKALSTASPYSLATIMLIMQPVVQYLFQILFESKRSNVYISSNITKNQYYDLLYIEDFFELSQLAQKNKQIEIEFIQKLVLHTNRCNSTKCQCRKIEPQKILQFNQIVIMISCLFKGSFEKYKHHQLNLRVFEIFSLKFLTFINKYKHNAPKSYQELKILFQKKRDYSFYFIQMCLVLQQILQAQLRKDEDYNINKDTRVSNVKLQVSKSEHSIVQDLYQMEQVKNNIIPLLLQVGQFKQQFWKSFKEGKFSEYSQIEKEVRKLQQLRDNLILQFNVYQPIFYHNGRTFNVQFLKFNALINLLLFNNLRKYFELEKERKEILQFEKSMNSFEITNINFFKGEAISVKVCIAFGPNIGKVMNKVISPLIPKFFGFGHFQHPLNSFIDYTKGNINQLMPAWLEPVHDEMMQNYIRRGVTARIGKYFQTFAKLYDKTLIKCQVYLAHNFSQELEDDFTMIGCLKSLEEEQPKFIGDDGKRLKNVAFKGYQYVLFDLNGNIMGITRGIYKMIEKMQRLVVNKGTYHEIDQFKSSNNISKSDNSSQESESYNAFDQKWSNIELSIDDFYGKVFIWMILPFISREIESTGIEFLMNGQTPPKNRYQNLTDLDISNLLVQNKETYLFIPEDINLFVQQYEKILQKIVDDVRVQSNNFSSGSAYKAQKSDYQEIDQFNTVTIFNEKLCVFFYDEHLKRHQTLNFGQTRQSEMVKVQGKPSSRSSIQEKQSSNSESQEDSEQLKTRAERIYQDRYSRYIEKFSPQDFHPVPVMYSVSYEEYRYRKNEIDHKQQIFVVELVVNEQQLLNTEKGYRRQLRDTIKQIYQNYQSKKQLLEQQTDEEDSISVQANFSEQKSVHDKEITNYQFPSHPSHYFDEAFLFDNKEVLITDQIFSPKQDDKGDFFLLSNRSKQSKPLLEVTERNSKDSNKKNKASIQSEHSVGDAQFKGSKLNITEKEIAQSVFIKFSQRKKQQEFYNENESKLPQESKDSQQQIFEEYQVKYTENLKLFETQYNLVQSQLNIISNPYSYRIQKYLFSATFILIVCFIILISTSTQGQYDLTNCLNLIELMITTQTSFSQITHSLYRNEITNQLQIKDMEILKQFYNNQFFGQLQELINIQNDYAIKINQANLNVEQFFDIENRILKNITLQEVIQLSLGQFYKLKQTNNNHSFIVDFSMTFASVANLKEIGELPQKAHQQCYQDNLNDHQYSQNILIVYMVITFFLVMMLQFTQIPLISKLRRNHRTLYKEIIKLQPLEVNEEIEIYDGVVNIFKKSIYEWMLIDFVQETQMFDINRQRKSIAHTPGLQKINNPDLGQSSNSNKKNTNKLMEKLKKSKMNQNKYISILMAGLLVILAYFLVVFLIIFMLSKDIISNIDAIFQSKLAQSSIINLINNVDLLAYSTLSNNIYDNIMNISQFQTYSEVLSDNSTDMFFEKYKNQLLSSFVDESLYNNFNILNEKNICTQEVGIDCSLTDAISLNPGIIANYEQGMKSLLTQVSTIIAQYPQFYETENIQNSEQILIEFYNNQYHILYIDYGSEILIKAYKQLISIEKDLFNQLLDVYKKLLLIFTLSVGLFGLIIILFLGKYLLKMQKDSIDTCLTALLLVSPKRYLSKQLGLVIQKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VVS0</accession>
    <name>A0A8S1VVS0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Kelch motif family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0730052</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000073">
        <property type="protein sequence ID" value="CAD8179899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VVS0"/>
    <dbReference type="OrthoDB" id="286745at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093:SF18">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13415">
        <property type="entry name" value="Beta-prop_FBX42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="313"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="321"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="344"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="371"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8179899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="512" mass="59734" checksum="18DA34BEFFD48164" modified="2022-10-12" version="1">MLSNRSNKDTKEVKRISLIPSRQEQQQEQEYKYIQLVKTVNVENQDPKWSELKVDGKNINHRAYTSITIHNDFLYLYGGYQVQLGIMDEFYRMNLKSLSYQWEKLIYKENPGPRTRHQMCTYMDRIYIFGGQIHQSVSTNSMWYFDLNSHTWIKCKINQSYPPEIDNHTAIIHNDNWIVFGGFFGGTVGLHSNYVYKYEFASNTWQRIQPQNSTAPAPRDGAGITIHKNILYMFGGSNGHQRFNDLWKFDFQVWTYIPVSSNILPKVRSGHVMSIHDDKIIIFGGIHDITWELDDLFVFNLKKMEWISVDEDSARKKDKQQLSPNKDNKQDLQNSRRQFRKSTRTGSIKKTIKRTTLSPLKKPDQSEDSVDSPTRNLSQAQSNQKTLDEKKKKEIQQKKMALLKIFEVDEGQKQQLRDNSPTSEKMRNSLFLVGNPKADLKIRQGKLTEFGKPLVSRFLQPLQSMQNSIVGKKPCARDGHSFTKYESQLLIFGGDRHQMSFNDIYSLDLTKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VWX3</accession>
    <name>A0A8S1VWX3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0780172</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VWX3"/>
    <dbReference type="OrthoDB" id="439961at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="16"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8182388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="186" mass="22108" checksum="ECE90FD5ABC9D045" modified="2022-10-12" version="1">MAKSQRSKVMRRWRSLKREHLQNVKVNQDLHELNQKLEATIQNRFQRPTQPKNKFLHPDDPTAQIPKFKPQPIIDFRSQSLPYSATQFVGARRKPVQEVVFKPEPEVEEEIEMNDQPQNEEVQHDEADDLINAMEGVRVKSKREIRRENKARAMEEIIPLTKNKQIKKNRRKELQPARKSKKIITF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VXC0</accession>
    <name>A0A8S1VXC0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0740160</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000074">
        <property type="protein sequence ID" value="CAD8180512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VXC0"/>
    <dbReference type="OrthoDB" id="128536at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="7"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="6">
        <location>
            <begin position="132"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8180512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="592" mass="68729" checksum="056CFA39ACC5F099" modified="2022-10-12" version="1">MDKEFQCCQCQHEFNLQDRSPHVLPSCGHSICQLCVTQYLQSQLKLICNEDNIECQVDREFKFFPINQSIIVLLRKKRTVQRCHTYVTPEDAIPRISAEDVSEFSGDSQQNIQLNKNISQSHSCEILPIKENQGDLCPIHSKPLELVCQEDGEYICVNCALFGNHKFHNYKPIDAYVKEMEQTFYDITTIYEVVKELSSKIEQKIYAKEFETKIVQSKDAKLQSIDMKFNELFNELNLIKEELKSKIANNYNIQFDDNKRKIESEFTQLKDQADRWLSQTGHQLNDFFVEKIQNQKLKMDAKELGNSKENGSQLISKIQFQFAQMDTHVKLIGEQQQFYVPIENIKNQFPQENKQLDQLLLDREDKLTDSDIIYQDIKDEKLTQSMYISQQPTQPASPQYNVGKNHYFSQTLTPGQFQRKDRSVTMLQTEPSEVSAPNTNRNKLSSSKFAKVQKFNKDPKLHDKIINTLAMFEKFEMIDFSTLDVTEQLIQAIDEALKNKKIVKVLKMSKCKLTDDLFAKLLSVIEESSVTALHLQSNTLTEKALDYLLNIVKAKKHFECKQIYLNGNISITQAKAKKKIEEIKKYGIQVTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W1S8</accession>
    <name>A0A8S1W1S8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histone deacetylase</fullName>
            <ecNumber evidence="2">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0780087</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W1S8"/>
    <dbReference type="OrthoDB" id="424012at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09992">
        <property type="entry name" value="HDAC_classII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005522">
        <property type="entry name" value="IPK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF5">
        <property type="entry name" value="HISTONE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03770">
        <property type="entry name" value="IPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="9">
        <location>
            <begin position="35"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8182225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="643" mass="73785" checksum="379ED76752767B2E" modified="2022-10-12" version="1">MQQIQEEFAKLKIDTSITGFIHCQEFDKHFSNNEHHENPQRTQSIDQTIQDYLQSKNIKKQVEQLSQFKQCDINHLKLVYDQKYIDFVEGLFKDVGDKKITKDFVHLGDTYLCKTSAITARKCVQAVLEGVDKILTQEWKNAFCSVRPPGHHSGHKSQPNGFCIFNNVAIAAKYARQKYNVNKIVIFDWDVHHCDGTEAIFYEDPNTLVISIHRYDNKTFYPGSGDPNKIGGKNAEFKNINVGWNVNWKTDDAPGYDDYVYAFDRLLGPIIKQFGPDFIIISAGYDSAMGDPLGCIKNTREGYQYMTEQLSQICPKVLAVLEGGYNLDVTADCALATFQQLMGVPQEFPVNIQPLKCGLSAVTTTVDKQKEFWTCLSSKELIEYQRKYFGQAADLISGGHLSAFEIKDNLIIKTTKKAEFKFYSTLTDLSNPYYEENQRLIKFLPKLISFDDKTCSITMENLTYGLENGSILDLKIGYKTYHPNCSAEKRKKEMKKAAQCDQNQMGLRVGGIKIRDQNGELTINKHGSEAYHLIRNEKLMNDYIEQVFRSNLAEQPNREALQGCIKFIQELVEVLQTIKRMFRSTSILIIVDNISKKYQIKWIDFNYVMKLSDDDENPKEQMDNNIIGGLKYLISMLKQIEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W2E2</accession>
    <name>A0A8S1W2E2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0780119</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W2E2"/>
    <dbReference type="OrthoDB" id="292008at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035922698" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="16"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="496"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8182289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="561" mass="65823" checksum="9DC477027C7752E4" modified="2022-10-12" version="1">MIIVLIQILFTQTLSNRITNPPVKIIDESFTSNDFYRQASEIIEIPYGAYPGVFAIDLHQRVQGEISNKSINLGDFEKVEEDLLEEDGCFSIQRNIEISMKLKLWLDKKGLTRDIYCTDVALAEKFRKAYVVRNDFKILQVDLNQGLFKLSLDPIQTSFANMLDYDDENTDYHPYLVGDKKSNKVFIFTTNGGLSFEANSKLDIEFKVENQIKKRDKIYNVHYNEKHQLIFVACGYEGVDIYKINDGNLELLRDISANYLGIDSQIVDVNSNGEDRLYVLDLERGLKFYWIKNLMELVYEFTITISHAHSFDFYDNTFFIVAKTINKQDFAIEVFVNQVTYDYYINNYFYDEMTINDVNVFQYYAVLIGDDGHKIIYHSIYEGFLNENLVSHTTFYQPNLIKVKEFEFEGEEYSNQRIFAAISKYEFQLINIIMNMPKIECKYSDAGVRQFYVKINSTNCAQKEKENNLNYQFTLCNVSHSFTFTTTELAGNYNTYAYYTLTLYVLIVASIWVVVLLYYLIKKWGYKIKYLIWRKKIVDATAPYNESFDIKETELPEKQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W2Q7</accession>
    <name>A0A8S1W2Q7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0830173</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000083">
        <property type="protein sequence ID" value="CAD8184658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W2Q7"/>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001800">
        <property type="entry name" value="Rab family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8184658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="210" mass="23705" checksum="9EFF573838E7DF6E" modified="2022-10-12" version="1">MEADPFIKIIVVGEQGVGKTCLLNQYCYERFEANSPPTIGCDFTTKVTQTNGQTVRLQLWDIAGQEKYNAVSKLYVRGALGCLIVCDIKDPNSLEETLKWKSIIEENSDQNNIPIMLVQNKCDLVSDDEKTAYYHTLQGFAKTNKFFSCIQTSAKEGTGLKQLFTELIQEIIDKGLLVNQQKNFKQSTNVQLNSVQSQQNQQQKKDKSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W4D1</accession>
    <name>A0A8S1W4D1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0750096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000075">
        <property type="protein sequence ID" value="CAD8180896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W4D1"/>
    <dbReference type="OrthoDB" id="294378at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8180896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="336" mass="39018" checksum="CC62F1AA7AC70842" modified="2022-10-12" version="1">MGQGCCQKSITEPYLIQPPELMYPLTFKHSKTILIAPEDTQPQNIANIIFLEPSAYKDDTEVIFSKDAILCKIKPQIENEFVRHTISKFGKYRSPQYPNNEKITTYPPYQFNETNIIYVGGWKTGQRSGYGIQYWPSGSLYEGEWKNDKANGHGRMIHQNGDMYEGFWQQDQAEGQGIFYHNDGAYYQGEWKQDLQNGKGKEVWPDGAWYEGEFTKGKKEGRGKYIFTDGSYYEGEFVDNQIQGEGLYVWVDGRKYKGSWLQGKMNGKGEFIWPDGKRYVGKYKNGRKDSYGEMQFQDGSYYKGYWKEGNMHGAGTLKQQDQEKIGEWFDGKFQRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W7B1</accession>
    <name>A0A8S1W7B1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0810142</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000081">
        <property type="protein sequence ID" value="CAD8183719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W7B1"/>
    <dbReference type="OrthoDB" id="313124at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="317"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="473"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8183719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="498" mass="56729" checksum="7F58E7D99D2DEF18" modified="2022-10-12" version="1">MLTKISQSSTLNQQNQLKQQNYLNEQQLSQIVTKIEIVQRPLASGESLEIKQNGIPLINKFKEWFRQAFFHHYAIILNTIQNGQFILHQLKEGFTSKEITDEQRQKEYYTVYGSYDSSQLVKQFTVKELQDQNPQEYSVVSNSCIHYVNRVIDLINQNLKDVNTHVLERIEQKNLIAMARTFIQNAYQAVKQQKIKETLKQKLKNPTLHNEMLYELIRSSKNQNKMQIFGNCVIIAIAQILNELTNLIKPLSVLMITFGLYFIWSQSDESIFQKCVKSLHLLFFSLIGMPLLRYLCDFISDLCKKLQIIKIGTRETLFIGGCTITGVIGGAFIKYGYMPNPITSLSNQIATLQNNCQYLKPAVEITAAYISGREQVCISGCAYATELCSVSSSNKLYSDQICTLIESKETQPFFSGLYEKICNSKVVKTISTESNSNSFIKKIKTGAGILYESVCAKIPIVKQFLITQASNPIVVGGLVGFGIGTYLIYKYWKLRNQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W988</accession>
    <name>A0A8S1W988_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0850057</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W988"/>
    <dbReference type="OrthoDB" id="299926at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="158"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8185383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="199" mass="23112" checksum="BBD573219A87682C" modified="2022-10-12" version="1">MISPLSIDKPSFDLQKRKPFSPTEITQQQNLLQRKLQQQQSDIISTIQPKNAHLNNNNIQHGLQKLLKTSPYGQLIKQKSQIQIYNQDSISYNYQSGCSPPFQTSHNFFQTDQKQSNSNTQNSIQRRIPSNYRVTLHKKSFDNSSQLISCRLKDQQQISALQQLLQRTAHILQNYKDEIVKYEVENAKLKEKIAILKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WFA2</accession>
    <name>A0A8S1WFA2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0890194</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000089">
        <property type="protein sequence ID" value="CAD8187671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WFA2"/>
    <dbReference type="OrthoDB" id="292008at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="488"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8187671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="540" mass="63063" checksum="33EB68C6225D64F8" modified="2022-10-12" version="1">MLLILFSLVFAKRINGPVHKKLFEQISDTNYVHNIDSTLLLQYNSYPGVYSMELGEVLDGEYRPIKENVTLDPFTLPGCYQYQRRLIHKHLADQQIESDEQTFTDMYIDGDVVFVTRSDNALLKFEIILDLENRKIIGLEFKEKYENGGISQQHQLFCYNKVCIILGEEGQNFIYKDNKTSSINLTSIIGEFREFPKDVYFDEKSKIIYICYENSIVDVLQFTNDKTIEKVKTIIAGEKIVQIRTNPESTILYLLDQQVGLLSYQIVSLLEYKQTSFQIILKNCMAFDFYQNTFFIVAETQDGIPYALEVFSQEDTYYFNKIYSKDMDIYDVWVGEHFAILIGAEIHRVIYHSIYNKFIEQTEIPLFFQDINLVNVEEYRKWDNTSYNDQAIEQEPFQKIPLFYKQSFMVGISNNQISLFSIKSLLPWVQCKPTTTNNTQYSLKMNSTSCLSKQRENDHTAFKQCIILHNFTITGKEIYIYEEDQQKVIIFGSILIGTIIILILFVVGVILYGNYKINQYNPIQPDEQELQNVQVGNPES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WI35</accession>
    <name>A0A8S1WI35_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trichocyst matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0900064</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8187829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WI35"/>
    <dbReference type="OrthoDB" id="296964at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035840439" description="Trichocyst matrix protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="82"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="256"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8187829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="357" mass="40773" checksum="C040E189A551E0A4" modified="2022-10-12" version="1">MRIVIIALVICLATCAKLRSQSPQKLQAELQKSNYGRALLHLVELHSMAGGAVSELVDAIEELVNDLEEGLEFLDFNFQRRTNEHNALLVQLNQQIQQAQIDVSRSEDVIENLLIPRKEQLEVRIENLEEYQAQNRQKVDEENLTREQEHEAYEAQVAELNDATAAVDDALALLSTLNNPSLAQVKKFQNSLKKIEQSIKPRSKMAPFLKALITLASNQNFSDQGVLSQIVNVLNEFRNAIVDSINDYSLQEVQDQENFEARIVQLDAEFAEFQKQINALNVDLTATLEKIDQVTAFRDQRRLDQATYEQQLQLENDLYADEVQIYNDTKNEFQREQAISEQALSLVRSVDFTNIQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WNW6</accession>
    <name>A0A8S1WNW6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0950041</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000095">
        <property type="protein sequence ID" value="CAD8189919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WNW6"/>
    <dbReference type="OrthoDB" id="68483at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14008">
        <property type="entry name" value="STKc_LKB1_CaMKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000206">
        <property type="entry name" value="Serine/threonine-protein kinase Ssp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF77">
        <property type="entry name" value="SERINE THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="253"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8189919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="589" mass="67909" checksum="048C7BC6871F0A55" modified="2022-10-12" version="1">MNNNKLNLLEVLRKKRIEQQNEQIPKTPPILKQKSKEEIRKYLQQNPKTNLISLFKKKREDDNTPCTRQKLLKFYDSQGRSSILSPICAANHVPQKSVSDESTNALQITIPKRMSLISPRLQHLNSQTSILESPNEIESFIVGGSNASKLDLDEHYLRIENVDNPRIMRSQSQCQDEAQMAIIPNTLSVPMGKGPRRLQSFSVGDPFRNVINAAEQRRSVRLMRGLSGIHQPKETQILERSRDEAGRKKMNNFVILHELGRGAFGKVRLIYNEIDQQYYAMKIADKNKLKRKLLTKETSAYSLLEQEVAILKKVDHQNVVRLVEVIDDPEERKLYLIMEHVKKGSINSKQYWKSEGVNIDWDEGEKPPKISCEKIRKYLRHFLLGLDYLHNFARIVHRDIKPDNLLIDEFDNLKIADFGVAQMYDSSSADLIQGDVGTKAFLPPEAFKTSQVKGKPADIWAAGITFFILTQGEHPFPAKNVQQLKEAVLNNEIRFSKDTDQDLADFLSCCLKKDARQRYTLEQLMDHPFITMNGEEPLIEQEFSDENFFISENEISKALSKVTIRATVRVFAKLKQKLNQSRQRIKRQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WRN6</accession>
    <name>A0A8S1WRN6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cyclin-dependent kinase 2 homolog</fullName>
            <ecNumber evidence="2">2.7.11.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cell division control protein 2 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">cdc2-related kinase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPENT_87.1.T0980047</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">May form a complex composed of at least the catalytic subunit CRK2 and a cyclin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000098">
        <property type="protein sequence ID" value="CAD8191035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WRN6"/>
    <dbReference type="OrthoDB" id="284433at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010389">
        <property type="term" value="P:regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07829">
        <property type="entry name" value="STKc_CDK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000611">
        <property type="entry name" value="CMGC family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000737">
        <property type="entry name" value="Protein kinase putative (CRK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF254">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="18"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8191035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="310" mass="35780" checksum="B35511D3FBFD4BCD" modified="2022-10-12" version="1">MQVDQEESIQYNKFMQNFSKEEKLGEGTYGIVTRAFDKKRGKVVAIKKLKLDSCDDEGVPSTTIREIAILQKLKHGNIINLLEVKYFMQEKKILLIFESMQCDLRKYLDKNQTLSLNTIKLIVYQILLGLSFCHSRRVLHRDLKPQNILLNETMTLKLADFGLSRVFPFPMPKFTKEIATLWYRAPELMLGDDNYGTGVDIWAVGCIMAECLIGRPLLAGDSQVDMLFKMMELLGTPTDNSYVGLSKLPHFKVTFPKFIGKDLIEVIPVLEHDRKALEILQSMLQFNPGKRPQAKELLKHNWFDDIRNNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WRZ4</accession>
    <name>A0A8S1WRZ4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0990105</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000099">
        <property type="protein sequence ID" value="CAD8191570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WRZ4"/>
    <dbReference type="OrthoDB" id="286797at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8191570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="268" mass="31855" checksum="12B268E4371FD91C" modified="2022-10-12" version="1">MKKSPPHQKKEKSLEKQGDQDKQEKFEKSHKTLQPLDKVRIPRNVNFYLHFLQHPEITENDIEWVLQLRHWDANKMKELLSKIPNQPYSLADKKTIFSEKKHDVQDIKNKDNVASFMHLLNHRLGLSASGSLADFETGLRRYQDNDDNLSKREKGWWYIPKKDRHDFPEFPKPYKEQMELKKSFSTGKFNTMKFTYSGFEGMQELPPYTMKFKQGNCGSVKHLFGPDVRMSQGQWEEGLRDSYNKRIPQQNRNKEESKNKKKLDPIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WTL2</accession>
    <name>A0A8S1WTL2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0930021</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000093">
        <property type="protein sequence ID" value="CAD8189076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WTL2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8189076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="122" mass="14609" checksum="3717E34CC964E1FD" modified="2022-10-12" version="1">MDEKITTLQQRKFLYYFQNNNTVSQKFDSQSEQVFKNQQTQLIDIILCILITERFYIIEPIIIFQSFWVKQVDFQNQCDFLVGTTVKPFKRLSCNLFLAPEMFIQKIQWLLKDPSINLSLID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WUY7</accession>
    <name>A0A8S1WUY7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1020036</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000102">
        <property type="protein sequence ID" value="CAD8192551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WUY7"/>
    <dbReference type="OrthoDB" id="295447at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8192551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="117" mass="13684" checksum="835AE3110951EA28" modified="2022-10-12" version="1">MGSVDQTQFTFIKRRHFSKKRKQESKPRNYTYITSDAKKLFLQLFLFQSYKIHDAANKAGIKYSSAKTILFAHRKQYKQELIRDKLAANSIQTKCCGYKLKQSNQSDPIQLITRVGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X4M5</accession>
    <name>A0A8S1X4M5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DBF4-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1140019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000114">
        <property type="protein sequence ID" value="CAD8196913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X4M5"/>
    <dbReference type="OrthoDB" id="286781at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8196913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="288" mass="34017" checksum="74F6B00C09618122" modified="2022-10-12" version="1">MAGEKLQSSGKRKCQLKCPDHQFNLQPKSTKCWLKNITNIDFCPLYNDALSCYENIVPVFNQDEQQCLPGFILEVDRNDYIKSMQKYKEDHLKKVQEYTTKKTKLQIPKPGRKHRYCGVCRKPYVDYLDHIKSADHINSFNRHEFVHVILKIVNEDYKNKDENKIQNDICFDVATSVIPKKRGPKPKNQGQQIEPKKRGRKPAEPSISKRLKTQPIQEQYNPYRPQQTPYFPQHYIFPYPTQLNQMFAQLPYQQLVNIPFQIQLGQDLNIERKNEDMNGQIEEGPRFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X5B4</accession>
    <name>A0A8S1X5B4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PPENT_87.1.T1110080</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDO010000111">
        <property type="protein sequence ID" value="CAD8196020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X5B4"/>
    <dbReference type="OrthoDB" id="10009520at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031090">
        <property type="term" value="C:organelle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20335">
        <property type="entry name" value="BRcat_RBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000051">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="423"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="467"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="197"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="201"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="141"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAD8196020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="511" mass="61043" checksum="1A79611DAB246E00" modified="2022-10-12" version="1">MIPSEEFEEFEEVILQKKSQSDAQYTIKQATQNFNQKLPTPQEQHISPEKQHNLCKDSLNESEILLELEEAPGLKKQYSDTSYDCKINKLKSFANKIIKNNKNHHLNQELSSNKNQQQNPKSILPVQSKGKNDERFSIIKFEHHDQELKELYRQLEEVELKILEKKKRLKVLNPIDENEQFEQDQQQIEIKVKQSNEEDTCLICGEDNKEIIQVLKCQHGFCQDCYEQYLNDRIIYAKVINIPCPQEGCSEIFPESVIENIVSLTKFKKYLRFKLQIEIANDPNKKLCPSVDCDYYVERKNSHNDFVMCKCGTFVCLKCGQIAHFFQQCQQAIDQNFIIALNKYNIKSCPKCKTNIEKNEGCNHMTCRCKYEYCWVCLQKYYEHHYKFWSPRGCAIWSNGKFKTHKVVNHPDLMRRIFFLPRLILFLFRIIMILLKLLSIALFKSFSKPFFRINKKLYRSRKPRRCFFRAIYFFFVQLFIVILVIIIFPFYFLFYRIYKEIKRLCHKGCSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X6G7</accession>
    <name>A0A8S1X6G7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1140036</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000114">
        <property type="protein sequence ID" value="CAD8196947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X6G7"/>
    <dbReference type="OrthoDB" id="318647at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8196947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="152" mass="18196" checksum="D1506FCFDC527DF8" modified="2022-10-12" version="1">MNNQYENQIPNGIEYKIEQIYENLLPPAHFRSNIKFYDEEKEMRRDCESHYFSNYQIENIQYQLEIDKIKNEKEGSAQKVLPKSQLLDPQPYVKSQYGCKILKTKASNTKMELHLENDNKNNQKQQIYVFLGQVTGKFKTRLSNYELLEYNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X6P5</accession>
    <name>A0A8S1X6P5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1140013</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000114">
        <property type="protein sequence ID" value="CAD8196901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X6P5"/>
    <dbReference type="OrthoDB" id="668540at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010608">
        <property type="term" value="P:post-transcriptional regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF12">
        <property type="entry name" value="MATERNAL PROTEIN PUMILIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PUM-HD" evidence="4">
        <location>
            <begin position="96"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="158"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="194"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="263"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="335"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="26"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8196901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="439" mass="51297" checksum="E0B0D6764BD991CF" modified="2022-10-12" version="1">MQETEQSEKDMIGSLLDDDESECSEFRSSITKSQQHSQSPIKSFKDLKESPVLNEDPTQYEFPSVDPSNFYITVQPTFIEQRQHSLSQEFHSYMLPQQPIYYPCQDGYFSQKRTKKLQLQTEIDSTISQQCQDQYASLIIQQSFIQGNEYQREKIFKGLVDDLFLLSKHKFGNYVIQKIIENSNQNTRTLMFEQLNSHILELSYDRFGCRVIQKLLEFILIQQKVQLINQLKPYALILIFDQCGNHVIQKIIDLVTDADFIIDLVTNNVDKVVSHPYGCRIAQKCLEMFPNDKLQALYISLIPLCERLSFCQYGNYIVQHMINSGPPKGFEVIGKFIKQRIIEVSQDKYASNVAQRYITYAQDEDIASICKILMNQTVPPMLLILINNSFGNYVMQNFYSKCNDKQKEQIQIQVSKYEEHQFTQFGRHFLQYLARLHAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X7A4</accession>
    <name>A0A8S1X7A4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphodiesterase</fullName>
            <ecNumber evidence="5">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1140020</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="5">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDO010000114">
        <property type="protein sequence ID" value="CAD8196915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X7A4"/>
    <dbReference type="OrthoDB" id="342865at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="PDEase" evidence="7">
        <location>
            <begin position="280"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8196915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="609" mass="70632" checksum="644783947F826404" modified="2022-10-12" version="1">MAEEQHLNEDSKKNVGQMGAKVVPRGSKEISNILGDRRSSYNVDGIDHVVVDEITDQKQVGIPLIDKIVIILVVLFTLLVFINFSFTSNDAKEDPDIEKTLFITKIIELVILILFVLEISFRCINVGPITYFSDLWSVFDALIIVASIVLIILDLNLEGEAFTTISKVLRGIFRFLRLFLVFRKFNQVKKINNAGTRYVVRSPVEKVIEIMRDLADQFEDPDIIKQLNWGITHISNNTVYEPIIEGKKSEALGWLNQPQQQQQISQELKRSMSSDIQFTDESHLPEQLKQDFEQNILNLDYDYFSLFQKYDSSILTHLMCYYFQKEQLFSNLRISPDSFKKCVDKLALSYHKENLYHNVIHAFDVTHTVYFFIQRCNFKEIGKLSKLDYSILLLSAAAHDVDHPGLNNIFLSNTRHELAMTYNDKSPLEQHHASTLFRYIRETDLLVHFTLSDFKYFREKSINMILSTDNAMHGKDYNKLKARLASNDFDPGSKDKGICFDTLLHAADISNPFKPMKNYEQWTFRVLGEFWLQGDKEKELGLPVTMLCDRRTTNVAKSQLGFIDFMVLPYYNTLSQILPQLSEFIEQITENKKAWAEQIEHYQTLLNTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XB83</accession>
    <name>A0A8S1XB83_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF4378 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1180048</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000118">
        <property type="protein sequence ID" value="CAD8198278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XB83"/>
    <dbReference type="OrthoDB" id="304761at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="155"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="15"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="439"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="571"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="155"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="191"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="211"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="234"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="244"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="323"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8198278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1014" mass="118639" checksum="0D0ADAD5F88B4739" modified="2022-10-12" version="1">MNEFNDFKQLRQNYSNALEKNIQKLQGRLKALNNNIINSTSAQKITMTKNPTEVKSLRSSCLFPDNNLASPTDRFENSQVFANFNRYSSLDVPQANQGVQKSKQSPDVFENIFLKQSYIPYEQKQIKQANKTKQKNTQSNTLKITKKVEKNHFTHQMNSQSLQNIKKKDDISQDKTLKRNPEKKKVLIPSCAQSKTKNTSRSPQKQRVKQNKQQTPSQNLNKPLLNDILKKFVSSKTSLSRTISKSKSKSKSKSKSKSPEKESTSFKKKQKSRSREQAIHHYNEQITSQFSPIQDKFQMRNIDYSPSLLRQVRTLSKKQGISKSKQLLQQQQQQQITNPSPSKVSEIHTLRLKHLLREKSNKQDKKARNQIRTTESPIQIRDYKIDVPQIKSLEDQPKDDSKPSSVRLNTLSIQRSDEKDRSVLMNIFNELKNLSEDQIQQFQQILKQIRVERKDEELQTSISYLNQVVIENQKANNQTNQKNNILESHLKMALEEFPKKSHASSPMSNSSFIFKKEFLTNSQIDEICQQREKSIKLRVNSQIQAFQNLLQQQKISPRSFHHNKQLVQSWFSKQADQLQKFKQELQKVQKQSNTLLKKTEQDVIIVKDLKKDQMLVLSQLQQISFDQSEIKDESFLDQSDLQFSIPNSPKIVTPNTKFLNQINDKNEMKIVQQQSIQSSQQKQNQFSKPFQQDPKRKDFEISTFNEELFFGNQITRPLQVSPNNESKQVITDYREPQVQIYGLNYSLRVEEETEKIIEQLVDEASNSLNKIELIQQYAYQTNAGILTNLGVINNFINLMCFAILENNQEEFIKRMNTPYGRKPISRLRQLHGQDDYEGHQEIQPLYQIKQIMETQNQYEKIHNQAIFDAFNDALNTLRPFYQDEGIPYPWCIETNISQRLCYGIEDIPNILERARQKVFDWSKTLCGLLSDEIPVISVRQLNYEQLIIMQECQGNDSLAYINEERIQNCLSIELQEGEKKWFQIAQEYSEVLMEISDYLFEELTEEMVAEIFMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XEG7</accession>
    <name>A0A8S1XEG7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1210087</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000121">
        <property type="protein sequence ID" value="CAD8199253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XEG7"/>
    <dbReference type="OrthoDB" id="306838at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="27"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="288"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="396"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="293"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8199253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="492" mass="59337" checksum="B00A6EE51EDF7EBD" modified="2022-10-12" version="1">MKQLLKPSINFDFNDVIFEDTLTQKLSSKQNKAQFNHQQRKDEDMRNQKTNVRPWSSQRQMTDQYQGDQNQQLNGKNSKQNKQKQHFTQNMSAKQMKFDKNQVCTTSPCIQSNFYFPQQQHNILKKCTTNSQQQIIQQQLDNQNNNKLQQQNQDIIKNAGIFDWNDNDFENQFYITNKQYESIEKLNQEKFIQKQQKPDVIQISIKQNQEILKQQQKEILQKFDNIEQLRMNEKLKNNQVISQLVLLDTVKQNDNIKEGKLIEQTPINNIQKFIHYEQEYDEESFEIDEENKVDDKQNKTQKKLQENDVKQSKIKLDKVQDNSKKVKSNESLIKTSSQNKKQIIYKAKNVKERKQELINMRSDLEKYLLQNNEAISKMFQELQDTKDKERIMIEINTKRQEELSQARLTLKQLQEKFSRQQQIIEDLNQKEHYANQIIQKLTENKQKYNQQWEHQMEKYMACKVIARFLKGKRDRKLYTEFKRQSFINRLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XK29</accession>
    <name>A0A8S1XK29_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1280019</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000128">
        <property type="protein sequence ID" value="CAD8201485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XK29"/>
    <dbReference type="OrthoDB" id="293381at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8201485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="87" mass="9716" checksum="D5DC6B42FA36C231" modified="2022-10-12" version="1">MGNDIQRVQQLGDSIRQLREDMDALSKIAPQVEKMIALSSTLESTTKNINENMSKVSKTVSDVDEKVKKAKEIMDKIPGGNKLTSFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XL49</accession>
    <name>A0A8S1XL49_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carbohydrate-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1280108</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000128">
        <property type="protein sequence ID" value="CAD8201624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XL49"/>
    <dbReference type="OrthoDB" id="61321at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09620">
        <property type="entry name" value="CBM9_like_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8201624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="201" mass="23090" checksum="6D4D732094B1A5E1" modified="2022-10-12" version="1">MILIFAIIIVISNAVIDPITVENCKWGPTQYKLAHQNEATTENVEFTYFTICKSLNHKLVIDFFNVDSYIDAPLTKCNDPLFQHDVDEIFIGTKTEYLEIEISPNGALFYATIGNPNGSCSNLITTYRQCDEISYFAEYVTPTAWNAHIELPISMFTQPIYANFFRVNKMKDRSTHYMSYNLINSSPACFHRPDKFIQLIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XLC0</accession>
    <name>A0A8S1XLC0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA/rRNA methyltransferase SpoU type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1280143</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000128">
        <property type="protein sequence ID" value="CAD8201694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XLC0"/>
    <dbReference type="OrthoDB" id="270651at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18096">
        <property type="entry name" value="SpoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051259">
        <property type="entry name" value="rRNA_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43191:SF7">
        <property type="entry name" value="OBP33PEP LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43191">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="tRNA/rRNA methyltransferase SpoU type" evidence="4">
        <location>
            <begin position="8"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="173"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="204"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8201694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="236" mass="27364" checksum="5C9CB1D912F9B599" modified="2022-10-12" version="1">MQQQFEGYLLLHNIAKPKNIGTIIRNAAAFNLNKIFMIGKQGQTKKKFQIADGFSFFGNHGTFEQMNFHLFTNLKEAKDYFVQNNIFVCGVEIGEGSQNIVTNPFKGNTVFFLGNEGTGLIPEHKAICDHFVYIPQYTQKTASLNVGVASGIIFHHFALWAGFQEQQVEGEKFLDPEKQKEEREKKKQEMIEQGRERKKQKIEQQQQQQQQQQQQQQQQQQQNNHPQNKNDNNDNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XMI7</accession>
    <name>A0A8S1XMI7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1310003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000131">
        <property type="protein sequence ID" value="CAD8202375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XMI7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8202375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="169" mass="20309" checksum="C9F2F3610B50940E" modified="2022-10-12" version="1">MVHPDVYEKKAIALEKALLSIYYGRYQYNFATVINKSQVTIFLVKRQYKPLYIYITIQSELNEQDQLIKLCNEFIHKMIYIYIHSYIILNLAQALFKQRMFEQVIESWDEVIKKKERQQEYYVEKIKAVNEQNGYPEAIQICNIVINLQLDNEIDIQNWKSIKQSKSNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XMU8</accession>
    <name>A0A8S1XMU8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1310005</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000131">
        <property type="protein sequence ID" value="CAD8202379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XMU8"/>
    <dbReference type="OrthoDB" id="310676at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8202379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="407" mass="48608" checksum="7CCB7655D97DE2FC" modified="2022-10-12" version="1">MNKNIENKKNNIHIYEQKGIFSKIKQIIVILIGEIRGSFKYLRIGIQNNIHDINFYQEKAITYFTICQLKNLVEQKMLKMFHLIFQNVSDQDEYLQGRELMIKLELLQYSNNINLEKQLNFGIKEFKIIKTNSIKSFYTLNNGIINNEGWREIADCWGQGITYNKNDYSFYLEKVQALEQLEEYQEILECLNLGLKHNKYQLLLYRKKGEILEKMGKDQEIIDNYDQAISIMVHPGIYEEKAIALTKQQKWKEVIQFCNCDKQKLNNYFFLVKIQIQALSELNEQDQLIKLCNEFIHKNDMYIYKFKAQALFKQGLFEQVIESWDEAIKKNEKVQEFYVEKIKALNDQNRYPEAIQNCNLVINLQLDNQIEIQNWKMNILEKQSNIREALKISRIIGTIRKIYQKQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XRZ6</accession>
    <name>A0A8S1XRZ6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C1q domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1350078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000135">
        <property type="protein sequence ID" value="CAD8203880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XRZ6"/>
    <dbReference type="OrthoDB" id="65833at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40131">
        <property type="entry name" value="C1Q DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40131:SF1">
        <property type="entry name" value="C1Q DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="43"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8203880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="529" mass="61932" checksum="70AF5F82ABCA7B0F" modified="2022-10-12" version="1">MSSNTLLTYLQNQITQITLNIEHIQKDISLLHNQFEQKIFTAKRELNSRDDSTQIQIEQIKQQFDYTIKALEQKMLHIDENKMDQNEAYQMMESNRKNIQEDVDLLKNEMAYQKKYFDDLLNEKIRRLEISNLTETVNQFKDQLKQYYQEEQKQLNEHQTLTKSLLNANKKAFFQELERIVQQVDSMKIEINDQQQLFIKKPELETRINQIHLQLEQKSDLIEVQNALNTQQIDIAQRFQEFKDEVRTVLELQGNEFYQLINKKANHSEVMQLLQGKIDMETLLLHSEDKVPLKDFHQHMSLIENIAVDLGKKQDTNSFLEYKEANNLLIAEIQKDIQKKMSKRDFKDLLDKKANHEDVSKAFNEIQSILSSKFNYEDFNRFQSNQNTLNEQLCSQNIIGRWLFKGNFLSPGSLIPWNIQAINTLTENFLWDKDKPNIIVVAPGIYEITLGFFAKKKPKIDIMVNGETIINAVNNSSYVVHHSSGKLKDTKTSVTGLTMIDFISLPARARINVAYQGDLGEGFLCLRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XTS6</accession>
    <name>A0A8S1XTS6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1340077</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000134">
        <property type="protein sequence ID" value="CAD8203594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XTS6"/>
    <dbReference type="OrthoDB" id="1919336at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09487">
        <property type="entry name" value="SAM_superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="3">
        <location>
            <begin position="140"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="34"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="78"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="197"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="45"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="87"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8203594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="417" mass="48873" checksum="CD0215E311269D53" modified="2022-10-12" version="1">MLRKKSQSKGNTQKLQVEVDIMEQRIVGLKQILQSQKESAKEKMSTSQGSTRWSAASTNVPLRNYGKHVLEKHREVAKTFQQKNKLSKVPSRPSSRSSSFSKQSKQSETQSQQNQSYLTQSLNFAGKNSANENLKRALQVQQQYSNEVVKFLNSINLDKHAGVILENGFDEMELLKEISVDHLKDMNIPPMDARKLLNRVQKLVETEERKLLQSQKQAQRLKESQSHIQEITNSEIYDEEEQHRLFQEAVMEYRQSQQQQKEQKEQAIQHTEEILIEDESLGYISSRQNKKEEQQRNKMKFLLSGANEWKMFDIDYEQKQGTDQQPVIIDKTSCYQCYRLFDQKFGISNNGKEFCTKECLESFKLQNLYLCRSQSCAKQIDIDQAYYEIGQWFCNQDCFKQYQWESVNIRGRTHISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XVK0</accession>
    <name>A0A8S1XVK0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1370140</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000137">
        <property type="protein sequence ID" value="CAD8204558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XVK0"/>
    <dbReference type="OrthoDB" id="308820at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8204558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="521" mass="61468" checksum="6A4F638FDAC6A2EE" modified="2022-10-12" version="1">MGCCCQKQGQKGKNNIPIQQVPIIVNNQEKQLDIIIPNPPVHLPDSPLIKKQIKKKLDIREINLLESQIMDQLQSRTSEISNQSEIKKFEELIQILEKESTQFANKKGSIETQLTQSFIQDESNLQNYSYNQDFIGVKVTNKNICDIENKYDFSQIKQFTEYQGRITIVRLIQKQKSTICTYIGKIDQKYCCVKLIPMAKRIDIDKWINRVQIAQLSNDDHRFDISFQFYYYYKIETIMDEPQYCNCFIISSLEQYNVYTFSRHPSVTISEKAQVAFDIIPIIQGALNSNQELNSNQSDSEKVSELIFKRMKTVKRNNILISRLQNQERKLKILLSDWQLLLPEFEQIIPNKQLQEEDQLIEIMKTKISQINESVYNNLQEEASLTELVPKYFLKLLFFKLNFELLNDVNLDGFINEAEFQSKCEILRQFENDVEKVLQQNIEYQIKEGISIQNEPNQIKFMNKKSQQMCNLFQLNPDQNRKTIYEIYLIINKLKCEGMRSHFRDYNQTISNQNNVNQQNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XZB0</accession>
    <name>A0A8S1XZB0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1450037</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000145">
        <property type="protein sequence ID" value="CAD8206750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XZB0"/>
    <dbReference type="OrthoDB" id="307020at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8206750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="140" mass="16699" checksum="3991F05A8BA3DEEC" modified="2022-10-12" version="1">MNFYTLEFEDLAIQDLREQEQIQVNEEQICDYNESSNLELNKDYTFDGYLQEEPVLIFNIIEESPKKKNKSIFKKSKKIRKSDTETDFQSLKKSNTLISQNDVAKLQQCQKLKTIISQMEAILKQTRIHILNQYRRKQTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y1L6</accession>
    <name>A0A8S1Y1L6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1460070</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000146">
        <property type="protein sequence ID" value="CAD8207137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y1L6"/>
    <dbReference type="OrthoDB" id="284619at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009967">
        <property type="term" value="P:positive regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12362">
        <property type="entry name" value="RRM3_CELF1-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:002159">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000383">
        <property type="entry name" value="Sex lethal, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="16"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="108"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="274"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8207137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="378" mass="42565" checksum="7632C74601EA2F37" modified="2022-10-12" version="1">MSYVPSFVSTEQPLYLKLQISQIPAAWDEIKLKNFLSNQGYTVSDVQMLKKDDPKSKGAAIVKFNKMQEGEKATKQLKEVAVDGMQPLVLKWAEGEKERLGVAEETSPKIQIDGLQKDCEEAMIKEIFMKYGQVTDVQIQNQGQSSISAIVSYTFKESCLLAIKNNHNKNQLGDRPLDVRFLGQPATIAKSQYPETPANEEGDPIRDITKSYTRWLDQKKKPIYIHWETGEIVRSVSFGSIIYSPDGSKEIITKEKYLNDLATFNSKKTGPPGANLFVFHLPNEHKDSDLMDLFSSYGNVISARVMTDPKTGKSKGFGFVSFDKQESAQKAKEAMDGHLIDKKKLSVTFKQGDGTPCQPLQYQWLPSIQQVQFDPFDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y246</accession>
    <name>A0A8S1Y246_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1490066</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000149">
        <property type="protein sequence ID" value="CAD8207976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y246"/>
    <dbReference type="OrthoDB" id="512616at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15571">
        <property type="entry name" value="ePHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13771">
        <property type="entry name" value="zf-HC5HC2H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="5">
        <location>
            <begin position="247"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="180"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="370"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="388"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8207976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="478" mass="55852" checksum="35453A58EF6E313B" modified="2022-10-12" version="1">MNLYLEQAAEELDQAISDADQLLALTKATLQKFTNYLKRKQQEKMERRFHLNNITEDSNSMKQLDKQSYNQMKILEYFNKFHKDVDKQLRDLKEMIPSNPQTYVIEELNQQEQINKNIEQKAPPKQVQNEQKYFPLFKQTRNTGQSIQNSFIQNQPILGQSQTQSQLQLQQFQNLGQFQSQQNQNTQNNQQKVTTQVQNSNGNQLEQQTETIKPILKQKQEIKESTITFGRLQKFQYQKLIYPKQNEGNCVFCSLEANDQNLGPLLLIPSNSNSSEKYQLHEMCGLWSQNLVFFSNLEQCDPKNIDEEVEKSKKTKCFLCSRSGATICCAVCPQSFHFTCLMKSSQTGKLIENQFKFFCDKHKYKARLELQNTSDEEQQPKKKKKVQSKLNTSKSIIKNSPKRGKSPSSQQALLQQCLKKKDDDLKRQWITSDLIYFNLPQWASIEQKLNDLDQSLQQNKLNEAKNNIQSEIKQEQIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YA11</accession>
    <name>A0A8S1YA11_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1590082</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000159">
        <property type="protein sequence ID" value="CAD8210470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YA11"/>
    <dbReference type="OrthoDB" id="286463at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="102"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8210470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="184" mass="21268" checksum="FCD2F798D67564D9" modified="2022-10-12" version="1">MNQQNLDKDEIALLQKPWVKNHTTLTNDDILELYRLFNLYCNPRTRRIDLKDVMITAQHLGLVEKSPIVANTLQQVSDQHGDGGVDFEEFVRELTNKLGNLFDQKGREQLFTLVDIDGKGTLDKDDLKKISDELHLNFSQKDIDEIIHNVAGYDAVDITAEQFQKYLGKLTNRRKIETEVLRTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YL64</accession>
    <name>A0A8S1YL64_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T2360003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000236">
        <property type="protein sequence ID" value="CAD8214679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="298777at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8214679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1067" mass="123375" checksum="EC8F2F6DD855FB9E" modified="2022-10-12" version="1">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</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RWE1</accession>
    <name>A0A8S1RWE1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0010252</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RWE1"/>
    <dbReference type="OrthoDB" id="290465at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8131720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="99" mass="11545" checksum="D295CADB967C1DDF" modified="2022-10-12" version="1">MFIIKTRKNSHPQPFSYYEIKILEVKSQPSQKALVSEQNQQNYLKLRLGTANTICVQGRSKTLAHIKKEKVIPFEMNYNLAYKKIFSQKNLFHLMGGNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RYI1</accession>
    <name>A0A8S1RYI1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0010664</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RYI1"/>
    <dbReference type="OrthoDB" id="5979581at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="10"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="314"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="314"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="325"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8132542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="426" mass="50036" checksum="3B660F1CBF0F2C14" modified="2022-10-12" version="1">MKQLKINNQFVTQKKISQGSFGIVMQGIDQKNGNQVAIKIERPENEHLRSLEREIEILSRLSNIKGVPQVFYYGWEDAYNVIVMELLQKDLSSILKQRKKFSLKSVFQLLIELVLILEEIHKQGVLHRDLKPENIMLDDINRVYLIDFGISKIYIPAHKGYELGRKDDLESLFYVMLYCLKGNLPWQNLKHVPDDQRTQKIGEIKENIEVKELFKDLPNEFIKIYEYLRKLTYASEPDYKTIVKLIQQAAKHSNIFLDNKYEWDIQMNNYDKNINRYGTLQTEELPLKQFEKFSSNLLNCNNLLAQTPSKFKQLQPPINKQESYQNSNSNNGSGNQNLSIYNSMGIIYQRSIDEISEELQEPIPDEMKSQNNICLKTLPDALKKPRLIFKTTQFDDDEQFKEDHFLSEKYHSLSAQVTTIFQNHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S110</accession>
    <name>A0A8S1S110_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0010645</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S110"/>
    <dbReference type="OrthoDB" id="162894at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05579">
        <property type="entry name" value="STKc_MAST_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000604">
        <property type="entry name" value="AGC protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000633">
        <property type="entry name" value="Protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000340">
        <property type="entry name" value="Serine threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE GREATWALL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="314"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8132504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="704" mass="81700" checksum="281CAF7F66DF0533" modified="2022-10-12" version="1">MDIHNQTCRLNAEQRKKLVQLNLQVARLCEKAYMKKHQIHLRQGLKFMKELRLRKNEGIQCCEDTRQVYQEYRILLLLISYGEKVVNQELDAKYHLITQNEIMAAQMSIDDQSILDLIEQMNSLINQRLDYCFKLKIQNFGSISFRVSKFSKDSPSSVRDSHRLNSFSDYISLNLTEETNESEKRTRSRPSTPARSSVSIMGNLKKSGKISQFLQRQQSAQTIKNQDESEQEKLSHFQTKKKSCFAQTASSESELDISIENSDQQMKTIESTEQILFEQDCFFASQKGYFSDSEIIKLDAENRNQERNICLKDFQFIRQIGQGAYGGVFLVKRIATGDHYALKIINCSNRPFERLLTQLKQERNIFEILTGEYVVKAFYSFQHQSSLCFVQEYMVGGDFAKILMIEGAFDENIARHYFAEILLALEYLHSNNIVHRDLKPENILLDQNGHIKLADFGLSELGFNKMMVKRKASQRDILKQDLSSSPGYIIQRGASFKKSRSNNSQYDKGSEEERRRIVGTPDYIAPEIIKGISSSNKTLDYWSLGVILFEFLVGIPPFNDESVDKIFSNILEGKIDWPDIGDDPESQISECVFDLLKQLLNPDFNQRIGHESIDQIKKHPFLHSINWNTLRNKPGPIIPRIPQAQQQQFENVQEKLQKFLQRGEKKHSQIVKKLQDELEYLERVDLLIATNEKEVLQIKQQYNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S2G8</accession>
    <name>A0A8S1S2G8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0020571</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S2G8"/>
    <dbReference type="OrthoDB" id="308916at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="339"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8133715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="385" mass="45980" checksum="A270A7EAFB6B3E74" modified="2022-10-12" version="1">MIIILVFLYLAHSLYLLEYKSNNLMINLDTSSTAIISLQGLISQIYDEDNDQYCPYGYNQCWMALQNNNTDTQERVQLKIKNFSIYDEEMNTYNTEFITFYNPPIDFEINHLNINFLTLSPLTQFFNSFVYFGYSLCRSGQDAYLKTRQNENLQKNRLNINGIKSQIYHQEDSVTYSIRISYLECFDQVFDISNYQITILDGIEIIILPIELFQRIKIFSKQNEFYEYQSDDENNFFYRDTNSLRVEFINPIKFYNVKEQPLLIMPEDYIFYNYENLEVDFLKLIGNSLKSEIILGSSFLKNKIIHFTSFTQEIFIEQLLEDKCNLQLNQKDVSIYQDLVKLIIILIFSIFIYAIFRRIRVKYLLKVQNKRVDQQLILNETQILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S6B9</accession>
    <name>A0A8S1S6B9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0040131</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S6B9"/>
    <dbReference type="OrthoDB" id="304926at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8135255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="69" mass="8471" checksum="F812C6F7FF345BB7" modified="2022-10-12" version="1">MNKVKEHIENNFNYQDMIEEEVEMNEQYLYEDMSFTQKPIENKNFTLSNILNQLNIDDHNNLQIEECHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S6Z4</accession>
    <name>A0A8S1S6Z4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0040545</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8136026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S6Z4"/>
    <dbReference type="OrthoDB" id="21225at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="148"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="402"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="790"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="847"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8136026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="916" mass="105825" checksum="9C39B6CDDBF20B2C" modified="2022-10-12" version="1">MRSINNSIDTPSIKVRFNNNERVDEMKTTKTIKKMNIQDELSIIWEYYMYMIMAILVEIIVLIFNNETKILNIIRITIFILIFIISLVYIKKMQQNLLLCLICLIKIFQTSTICLNNEVYLIAIIPFAYDQITINNKVAIKLKVLEQILIKIIGVSLICIFYKLPLAITINLCLMILEIYRNRIIFMKTEVNQSLINYKIPIDYEQVAQSVRVENTQREDIWKKRFYIIPLSILLISKETLKITYKNQTLFKQFCDSFINEEELEDLILNKLYFSLLSDHQEFVQLSSKNIIKLKQKKSYSSNVFHQKSCMTQLSSYDLMQKRTLIEILTQIKSDMYNLQKDVIELFCQQTEISGAKYQLEAKIILSDKDDEFFVTLNDTTKQNEIQQFIVKEEFKSKIIESFSHELRTPLNSAINFLEASISNNTIDDKLKLQTLEPAVNSLKLQSYLINDIIDYQNYNANQLELYVTEFSVQDFLSELTSLFSIQFDMKKITFNLDLQKNQLQYFSSDQTRLTQILVNLLQNSLKYTSSGVIKLKFSSVSNDIVKIVIQDSGIGIAPNVLEQVRITLKYVEESKDFQTFKAWKGFGLLIFALLHQSLCPPTLRSIQIESTVFNKGTKIKFYILNFNYSSKQNTIKQSFSAKNTLRQSQSVKKSLQSNSLSQILPMNGTIYLAQDIQQVNQHMKKSERIMNSVSNTLDDFSELSKSPKIQFCSPDANISQLIQIQPVLITDIKSSQKISIKNKNPLFQSYKSQKSQLVNLNQFRKMETQYREDIFKTLAIKKNKCNCNIILSVDDEIFNQKSVERLLQQIGFDVKLAFSGDEAINLILNLKPCSLGCNILTIILMDYQMPRKDGIATTKELKKLMSDGIIPEIPIIGLTAFTGQQDIKNCLIAGMSDVLSKPLKIQELKDVLASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SF85</accession>
    <name>A0A8S1SF85_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0060240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SF85"/>
    <dbReference type="OrthoDB" id="311773at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="182"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8137722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="323" mass="38151" checksum="8BE37C6D8DA37A67" modified="2022-10-12" version="1">MTKILTKQLIIQKAKTDVMENIKSINLWGNDLEEISFITQLKNLELAQLASNKINTLKDVVKCSHLKDLNLRNNNISNIEEFQLLKQLPNLKALNLLYNPVTQHPNYRQQVLKYASQLEKLDDIVISQQERRQLQQEEEKENQIKQNVQMDYQKIKKIESKIFKKKSKENKSKNKKSEDSQQELNKNQENNKSQKGIQQFFYEESQEQQEIQIYDQKEFQFQNENKNIPMLVGGKTHSQFQINDKIQEEEEIPFQIQKSEQITKIQQTNQNSKTNLNFNGKPDSFETSQHILCAILSLLEELKQKDIEILKKKVESKLQRDMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SIM7</accession>
    <name>A0A8S1SIM7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0070225</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SIM7"/>
    <dbReference type="OrthoDB" id="309189at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="273"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="378"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="487"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="302"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="328"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="393"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="411"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="445"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="487"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8138642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="683" mass="80384" checksum="973B30793ADA9B26" modified="2022-10-12" version="1">MLDYIPFTKDKDSDTKVQNNIGGGHNSILKNQKYTLKFLQNKQNYAKINKVIDYQSLLNSPEQITTRSLDIQQYEYFLRKDGLENRLKEYKISKGQQTTLTRKQLQQSIKVENVHLNENSDHQKKLELALNRSKFNRVGSIGESYRSQDSLSSIQKITRYQQLQQQTQYLLLQQQEEQNRQKSNQEDQSLSSIKVEEQIVDISTEKHEEVVLNEPIENDNSIQYESQQQVEIQENPQQLQINESYAISQFSVPQQYSINNEISLINYEDDEDIEEEEVEEQVQSAKKQTKNKKQPRQKAQKQQKQQQSNSKANQSIQSVKKSDGIRSNKISRQQSQMSISDLDRIEQIIQQNANDLDEAEELQRDKMRKQIRELEERIQKTKERQEQFQYNPEEYKDMSGEYLQTKKKEQQQQQEQQPQQQQQQQQQQQQQQQQQQQQQQQQQQSNQVQPHQSVNTPKKQGKQEIRPNNQTQLIISKQAKQIKVPETQSQAVLPNINQNQRIQEHPMTAKSPRQDKKNQDINELESQRISQHSIELERSVVESLKDLEKQQKYSSPIVSDPMIKIENKLKQMQEDMEVHQRKQSDLDFADFLLTSGNVFDTNYEKYKIFQVDKKPDDPPGHFGEEDKNLYQQMKYWDAQNTGNSKKISTVADIVRGICTKLTSDANLSEADIKTQKTIDSDWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SKA3</accession>
    <name>A0A8S1SKA3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0070331</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SKA3"/>
    <dbReference type="OrthoDB" id="10252009at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000118">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF519">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE MPK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="5">
        <location>
            <begin position="18"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="6">
        <location>
            <begin position="78"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8138824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="299" mass="34325" checksum="5BDBC0E11857C23B" modified="2022-10-12" version="1">MKKQQLLVNVNEMDLILDKTTTQGALWLGNLKAAQNIKQLKENNIQTVITVANNIIVSFKNSLNISHKIYKVEDTEKAQIIEYFDEINKEISTGLNSGSVLVHCAAGISRSSACIIAYLMKTYQWPYEKTFYFVKEKRLAINPNPGFKKQLIQYSKDLQANSQNQNPNFKETKNIKDSDPPILGYSNSWVKLNKKKFSNPHELVNQLSQNLVKSPLKKIGQLNRTSTPEERQIYRQFLAKKLFVNTFSTKNSVSTTNYQSEKSTQEQKTIEKQPIKIKALLSMYKKVEINVLESERTQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SMI3</accession>
    <name>A0A8S1SMI3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0090413</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8141050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SMI3"/>
    <dbReference type="OrthoDB" id="285459at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8141050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="211" mass="25629" checksum="21935576AD3C65C6" modified="2022-10-12" version="1">MSDLRDFDIKQLHNNIQSCFQYLDKQIYRAMNIAPINNTIIIEKPNTTIAHEYKELQHHVKQLQVKYDEVCYKYMQLLTKYQRTKITLKCQEEEIEHQSELIKEIKLMHLQKPEVIQIPVYEYLREFTKNNHSQLSNFLQILIHTKKMDLSMIELLQNIEKYYRPQNKMVFQDKYIYPKVPLRKIRERILKTLPDQTVSQNLIPERSISFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SQ54</accession>
    <name>A0A8S1SQ54_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0100056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SQ54"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8141327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="138" mass="16586" checksum="375C590155FC4A86" modified="2022-10-12" version="1">MKNNTQQSTVSPKMTMYKNAMKNKHNQSTRLGEQLQKNQSLAQFQEQQQNQRNKDQEKLKDILEQNRKVIKINQIQSNIQHLDQQQKSFTTQNQSFSEVFQEQQIDNKKLYLPIQECKQRFKENVNFDFETPRFFGMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SRT6</accession>
    <name>A0A8S1SRT6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0090473</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8141164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SRT6"/>
    <dbReference type="OrthoDB" id="536211at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8141164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="148" mass="17379" checksum="041ED5352EB85D38" modified="2022-10-12" version="1">MLNDQFIIQNVLISLQMYKLQVSLIKNHKNQIYESGYYADEINATCLKQCRGYIKANEKQCDDGNIIKGDGCDYQNELENMYIIVKGGSIIRLNFLIIQCQIMENPYQRVQVLLKFTLLFDFSNYILLLSLLLMKVFKLKTIQHFINH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SSC7</accession>
    <name>A0A8S1SSC7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0110352</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Fe(II)-heme o + 2 A + H2O = Fe(II)-heme a + 2 AH2</text>
            <dbReference type="Rhea" id="RHEA:63388"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:60530"/>
            <dbReference type="ChEBI" id="CHEBI:61715"/>
            <dbReference type="EC" id="1.17.99.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:63389"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Porphyrin-containing compound metabolism; heme A biosynthesis; heme A from heme O: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SSC7"/>
    <dbReference type="OrthoDB" id="1726137at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120547">
        <property type="term" value="F:heme A synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016653">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006784">
        <property type="term" value="P:heme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003780">
        <property type="entry name" value="COX15/CtaA_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023754">
        <property type="entry name" value="HemeA_Synthase_type2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23289">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23289:SF2">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02628">
        <property type="entry name" value="COX15-CtaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="210"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="322"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="350"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="381"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8142818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="413" mass="47480" checksum="BC9D68A4CA8BFA72" modified="2022-10-12" version="1">MLLSRIRTFKNLTTTLKPLQNNRYYKFSNAEAAKNAKFDRNAHFEDPVNEWVDPSKRWLLGGWLLLCAGGVYFMVLLGGYTRLTHSGLSMTKWKPIEYTYPRNQTDWEKEFDYYKQFPEYQHTPIDLDKFKKIFIVEYLHRVSGSTLGAIYVLPLAAFTAMKWIKPQYAKRLGAIGGLGLLQGLIGWWMVKSGLDRPKPEYQQKPRVSTYRLTVHLSMALSLYSLLIWNAATLFRKPQHLQITPENYKTHLKFRGLGIGLIHLVAINLLTGAAMAGIDAGKVFNTWPTMNGQIVPANLFKASPFYKNFFENVVMVQFNHRNAAYLTYAFGSYITYLAYKSNLPKQARYICYALYGVLNYQLLTGVVALLNQVQVHSGSIHQFNALNVLTASLLLCHNVRRPSLPYRNYIKQFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1STM8</accession>
    <name>A0A8S1STM8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0100351</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1STM8"/>
    <dbReference type="OrthoDB" id="303345at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8141874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="170" mass="19937" checksum="A14A9B8B769C800C" modified="2022-10-12" version="1">MDQDQPKKAIKRAYCKKVKPQILFRSESEKMRQLLQIIDDNNSLENLDKQVALASSNSQLKNIQEQKQSSQTQSSDKNTQNTTPIQTLIQPSTEQTPKQTLEIKIIKNAQPQELINETQKKKLKPDENYEQRFCQMKCELQSFLTYWIMKLKSSVIDTQQMVEQLEQFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SYA4</accession>
    <name>A0A8S1SYA4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0130152</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SYA4"/>
    <dbReference type="OrthoDB" id="2121326at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45663">
        <property type="entry name" value="GEO12009P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45663:SF11">
        <property type="entry name" value="GEO12009P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14561">
        <property type="entry name" value="TPR_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="63"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8144286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="356" mass="42022" checksum="E05308A50909ACD1" modified="2022-10-12" version="1">MIRKLFRTPIFKSLKFYAPALYMFSQQNQDPKFSEEIMNKLKNQAQQFYDNIVVDNKLQHVDVIDTDHFNQLVQESNQKIIIVYCFAQWSLECKEFKEVLTKNMEKYSDVSTIINIDIDKHPNLITQLQINSVPFAGVVYNNQFVDGYFKNGKFDRFMSIVEQISRILRGENIEEMILEQMSGLFQSRNYQGLLTLTKEALARENIKKEHPKYLLFQALAYVLMEDSNKIQETLDLFDKQYKDDLEDMNTKQLYQLVQDQFQMIKDLQSLTPEIKRILERLNDNPQDKDLYFDLALEAINAKNYELAIKTLLNIVKMDKNWADKKAQKKLIELFTQLGNQHNLVIEGRKQLGKLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SZP8</accession>
    <name>A0A8S1SZP8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0130365</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SZP8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8144717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="86" mass="9850" checksum="07D06BFB9D063CFF" modified="2022-10-12" version="1">MIKLVNCFGIDILCFANSYCGVLLKQNQKLKKACNLGLIISAFSFQKQKLLIVRNKPLILTVSIILMQGFKVCRFIFIFFQQSMVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T2I4</accession>
    <name>A0A8S1T2I4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0150063</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8145878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T2I4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8145878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="74" mass="9012" checksum="0D775FC2863B51C9" modified="2022-10-12" version="1">MKYLKDKQKERHYILEKVQLSIKASEDKEVNLKEFMYLKIQMKLQQSNNTVQECEIIQNQNINEQAQREIIKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TA44</accession>
    <name>A0A8S1TA44_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0190025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TA44"/>
    <dbReference type="OrthoDB" id="307579at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="386"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8149270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="520" mass="61039" checksum="7C7B72A52BC5D101" modified="2022-10-12" version="1">MKNQRISKKLNNCEIAINRIKSKFRMSTILIIYSFFILAFTFANLAICLHMQITLTSNQIEEISNQQLKLQNSKALNIQSQETTHLFQIAFQMAISKMEKLTTLNLITSIQGVDTVKNISSCHVAQQFDDSIQQVTLCLSNSATQEQQTNYSKQFISFLNVFESLQQIFDFPIISKQLYLVSPYDHFLTAYYPIKLKNETKELVQYDWFINYTTEIQQSQNPSFFKLIPYVKLQNYEYLFAALTILMIDKNHQINGIGVQIINFPLLSRILFIENLNIILVYEDGKIMYTQSYINNGLEKEIRYIFNETVSGFNYSDWELIKYSLNLNYSIQIYNRLLNQNVYIKAKQIPDTPLISLILTNITYENEMQKLLHEQIDSIQNWQLTLISYSTLASILIILLSLIPLRSLFKPTQVVIEMMIKYLLGKFDYKLKDQVIRENEIQEMNNVINQFYQAYQRVDITLNQSQYVKNEHCMLIEKFFYQQNIGTMLNLSFENLKNNNEQISLSEFQQFIAISNFVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TB48</accession>
    <name>A0A8S1TB48_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0190221</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GPAT/DAPAT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TB48"/>
    <dbReference type="OrthoDB" id="429813at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004366">
        <property type="term" value="F:glycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006072">
        <property type="term" value="P:glycerol-3-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05236">
        <property type="entry name" value="FAR-N_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09071">
        <property type="entry name" value="FAR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07993">
        <property type="entry name" value="LPLAT_DHAPAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000458">
        <property type="entry name" value="Fatty acyl-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033640">
        <property type="entry name" value="FAR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022284">
        <property type="entry name" value="GPAT/DHAPAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045520">
        <property type="entry name" value="GPAT/DHAPAT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041728">
        <property type="entry name" value="GPAT/DHAPAT_LPLAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12563:SF17">
        <property type="entry name" value="DIHYDROXYACETONE PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12563">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19277">
        <property type="entry name" value="GPAT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03015">
        <property type="entry name" value="Sterile"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="6">
        <location>
            <begin position="599"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8149660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1138" mass="132035" checksum="922F814A590B7954" modified="2022-10-12" version="1">MLQEYYNRKVLFITGCTGFVGKVLLEKTLRCLPNVECIYVLIRQKKGSSLMERFKREILDSQCFDRLRQIYGGGFEKFIIEKIIPIEGDMLKEGLGMAEHDKNIIINNVNIIINCAASVDFNARLDDAIQINVRGPQRFIALAQQIKNLENFIHISTAYVNSDKAGYIEEKIYDPNQENLEQLVSQLLKTPVSILEKNVKDIIGDFPNTYTFTKCIAEKLLVQNRAPNFPLTFVRPSIVGASWKDPTPGWIDSLVASSAIFFFVGLGLIKTLNGDECLIGDQVPVDYVADFILATGAYQNGRKEVSVYHCCSSAKNPMTWQLAKEVNALFWTKSPSSQQFSKPNLTFYKNEKIYKIMSKVKNAPALLYYQVANKIGNKEMKIQAKRLRKIIDRAESINDTFKPFVINEWIFDSSNSNVLIKFLSESDKLNFNIDIEQLNWRQYLERFNWGIQKYILKDQTRELNEQSTDVLSQRNQQSYFSDIEWCLSHGQDFKTKTTKEQISLVMNSQRVQSVIKQLVEEKSKKYQQTTLNLEKLHQNIEKQGIDICEVMFANYNMRIIRIFGWFITKVFRQIYEKVQINEPALLELQNYDQKTRGPLIFMPTHRSYIDFIMCSYVFFSYKIKCPHIAAAEDFLSMALIPTILRASGAFFLKRKQLEDNILYKAIFYEYVQRILIEECYLEFFIEGTRSRTGKTLNPKFGLLNIVCDAVFDKKIHDATILPITINYEKVLEADTYPYELLGEEKVKESLLRVIKAIKILSSNFGRIHVGFGKMISVKEWSKQQGLDSFENIRDRKKGVETLGYEVAYQLIEEMVVMPTGIVSTLLLMNRRGITEDQLIKRFEWVLRQITKRGAKTSITNNGQSDVAVRNSIGFLQDLVDKKKKNVFELTLIPKQEYKSILLLSYYRNQLAHIFFSEGIVCCALNGFGHLLSHKEGVSIERLWEESDFLLKLLKREYVIRNRITSKEQMTNFIQQMIDKNVLEKVQEKIRVNQQFGEQASQFTCSIIWPLVECYWATIVYLYQLKRVSDTNTTFELSELITQIQGFAEQMYGEHIMEHYESCSIETIKNAINTYQTMRCINISKKDDSDQVSIVFTDQELLEVEAQVKKYLKNNYSKSIQNPIDIARSLLDYTIIPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TBA9</accession>
    <name>A0A8S1TBA9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0180424</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8149118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TBA9"/>
    <dbReference type="OrthoDB" id="68483at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:002298">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF77">
        <property type="entry name" value="SERINE THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="28"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8149118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="327" mass="38558" checksum="4FA623854F75B3A9" modified="2022-10-12" version="1">MDNEIYEANFRNKVKHRKENGKKFINEYELLDELGQGSFGKVKRVTRYFKETEEQEELSKSDYAMKIFHKTVLAHQRTCFYESNSNNPKMTNLLEFADNEINIHKYLNHPNICKLYEIIDDEDDPQQKIYLIMQLGDLGCIMNFCEITFKYIRNQAILDYLKMNYMQAAKLLFKQIAQAIQYLHENNIVNRDIKIDNIICTTLYPLDQCCKLIDFSTSRIADKDSIFYDCAGTPGFRAPEVQFCLNDGYSPFKLDVWSFGICLYIYTHEKLPFWGEGDLETDLLARDQPLQFEVQDDLLINLINSCCAKKPQDRPTIQQILDHEWFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TBN3</accession>
    <name>A0A8S1TBN3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">MIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0190004</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="76898at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23263">
        <property type="entry name" value="beta-trefoil_MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013662">
        <property type="entry name" value="RIH_assoc-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000699">
        <property type="entry name" value="RIH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015925">
        <property type="entry name" value="Ryanodine_IP3_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13715:SF99">
        <property type="entry name" value="INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR-LIKE PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13715">
        <property type="entry name" value="RYANODINE RECEPTOR AND IP3 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08454">
        <property type="entry name" value="RIH_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01365">
        <property type="entry name" value="RYDR_ITPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2600"/>
            <end position="2617"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2649"/>
            <end position="2670"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2694"/>
            <end position="2714"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2720"/>
            <end position="2744"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2765"/>
            <end position="2789"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="12">
        <location>
            <begin position="113"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="12">
        <location>
            <begin position="368"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1023"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="2266"/>
            <end position="2291"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="2957"/>
            <end position="2984"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1034"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="2266"/>
            <end position="2283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8149228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="3001" mass="350735" checksum="68FCB905266AB2D1" modified="2022-10-12" version="1">MSNKGESLKFGQVISISPLANQSLFVYSDGLVKTDAKLVEISPDIMAAGSLYYYSLYRIYPDFITTKLQMAINFKEETMQDDNSKKMQKLKDLQTKIIADYKSNIDVSTKYKDKPLSFNNLIQFMHVATNRFLSLSSKEAKDEKENFRLELVEYPSSKTVFQLQPAFVHQKESNSEVYAGDSVYIMCDHIYLGIKPKLRATLKYGVMDTYEYLQDRSIYAVPNLKLLKKMTDQLAQQQDVLEVTPTIQKFQKVASLAKGQSIRRQTTLKQEEEVRPIGQTSEVNISLEHQSPWVLTVFCNQNDNEQIISFNDVIWIHHIEENSLLTLGRNQDNELIITFQKQSNEQQSEFNGDSNGMFQIENEDLNKGGLVEWGQSLRLRHFILGKYLAINYEPIKTGQLLLYLSDQPTPNTLFQFLAVPTMGSNAVSQKYVTKDAFMKMKAVGKSSQWVHLEQKKILQANSKYKDTVVEEDKSVIVIRLTDNTEDDDVLKLYKANYSDVLETNFLITGFPVLRRGLTKLKSNKDMTKEAQKQQMIKTYKKLTTTIEKLEKFCQNSLINTQLDIKNTKVAILQRQKILREQYFIDLLIEIMKQITNEDELRQYSEYQLAQHKAALEKQMKTTIAVSQRSHIGSDISPLRQKDSQINSLNQIAVGRSAASQLVSSHAIQSQQRTQARKKKMEMAFLSEKMSVLKVIYRLLRSICQDNPENQFYIYKLFPNFLYQIKYFKEATDCIITVLSGNEQILINLCDHIKLNEKLKARQQVDSDESQLSDEMDIQFNSSADEKKNILLYYKNLTEDSQGQRNIQYLQFFRCICRLNDQSISVNQEMIFKFIKQNENFKSSLFMPIIIESTKMKICVDQTQIAYITSIKDPLIQNYLIEQLFLYADLAYQRNFLWKSYLERIFPTQFVFNQIFDEDKDQSKQSLGNSDQVFDKSMQINYKSAFCNMAITIYVDHEPFNKAIVPNLCRLYTETKPISKSVQSINKLTKLASTYTPLVEKTFKFLNELYDQINLDLDQKKEQQRSKGLQINQDPEDKKRQARNKDQQNEQESFINPLVLDITKLISIMIRFDMLKIMKKEDLYVNIVPKLIALLEYDKNNLLYSYILKSVRESKIKKASEGINLNVIGKVTAQSQFTKLAKDLALSILGIPPKQQGSLEDEFSDYDTTVIARNPIVSSLISIITYMDTLGVQETNSKNQNQQQIQNNFEIEIQKEVCGILLYFQDQRQDFYVSNFIQFYKNFTTKFQQFSWNNTLLKDKFLKDLESELVQALPPFLKTGIEAIDKQAQTSIEAIYKSNDMNELIGVGNLLAQQKKFQIQNPQLTETMDLDKLTSGETQAELQLLPTLIVTFFVTNDFGLQNELIELMRRSFSQVSEMMYHLNRIELLFDKVEIGCYQFLEVSIQELKVITECSEVWLVDFLASPQGEVKEIQKILKILADLQLLLKQGTSIDHKSRVINGTDQISVSRQKIFNFLNAYEPLLNFIRDTQQQLVKVLDDPFYSMETKSYVIQLLKALFALLTDFCDKNDENQKIMHKNASLFMDELQHDYGQMNLLSAIYRDNKQLCQKIDETLLKKIKSFIYAYGRQARFLHLYKNIQTYKNNPIIEVQTKVLQLFIPQHISTEDYTDINEFYLYGEFKAIEGRKVLEFTFERSHETKLCDQPFLYHSEILQVLLATIIGEESFKINSPKLKKLFKLHYLLELLLQPDDFLSEEANILEGLVDVNLGMISGIKHIKPQIIEMANLLYVNNKVTDLYLFIKEQHKFTEFFAQEKSRLVKIQSLQGDFEQYLQYFFDYVIVLMKYYSKKVITKDSGSEYRERTDYMELKDIMNIILGMCNTLIQHKLKVNQYNNLVEFVHSLDEQTLTDNAGQISQSLQQQEFAKPQVEEENQTIFTQKKKDSPLIHNEKDEKLKRTNNKTDIDQLNQEIVDWNTKKAWEKFLEECQQLQSTKKTQSIEMRILSQTFQEVHKFIPDAMIKELGLQNFCFKDFLKKLIRFIEVGIINKANKSNLIYTLQLLEDILDSKDDLEKIQNEFDSCQATRMFLNLLADFQVYPFDDDLLIQLFKFALKLSKNGNQRIQTTVFNYFSNFEKSEMIFLKMNQIIYEVINENKKTQQVLMQQKSQKDKDQHQKQLYLSSIQEQQNEDESTSKEAQEARRSKMSILIYLLKYFQLICEGHNLQIQNYLRQQYNSRNNYNIVQAMVELLCAFENDLQRDSFEVIMNTLETLTELVQGPCSQNQQDIIDSLFLDVASNYLNMHIESKKKKDQDGVQEKEEHSIRERSNSLQSKSTAKAGAKVKTIQIDRWMLERLKYKCMVLVSSLLELNSDTNAIKRILRSLPINVLKKNLIIIYKKHQKMYKKLGYNKESLGHLEENPHDSTKPPEYHELILETGFLIYFLINHYANLDTKEIDPATRDELEDIKESQKNSDKEDPWAQLQNSLIGQLASLVFSLVGGILEMINEWREYAQQQLMKQFKGKESGEEEEKRKAAKKLAAQQLFNDSIMFFQKNSATVEVIREQQIEKIIFYKLSFCNYLPQETKTEFHETVDRDSTNSKVSALVENMPNFMDVAKHEEELTQFFNQNKFVALFAKYVILWKDLCFLLTLVLNVFIVLSYYEGDSDDPDQRFYDRLNSPKFLKMLSVEETMSIFQYCGIGMIVCSCFVVSFFILKKGPLYVKEAWNQSKDLIKEDQIWIIRFFLKLYMVGFCLVKVLLNIDMIYYLAYGVLAFIATLVHPFFFAFHLSEVVLRYPTLRNIIKSFWEPKVALGLTFILVLLMNYYFTLMAYLLFSDIYTNGKCDSLLVCFLSTFDYAFKNNGGIGGWFDGNFPQDPANYSYGRFFFDQLNNILIQMISIQIFSGIIIDTFGELREQQLAKEDDKENICFICGLDREIFDMKSDDGFNKHIKQSHYMWNYVFYMAYMDNKNKSEYNGIETYIANKRKNQDNSWFPIGQSLELQEELEDQQMIEERKIQSLQQRIDGIKQQSEKVLQILNDIQEQVGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TC50</accession>
    <name>A0A8S1TC50_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0180386</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8149044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TC50"/>
    <dbReference type="OrthoDB" id="288536at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8149044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="104" mass="12274" checksum="9FA7D369E43212D1" modified="2022-10-12" version="1">MATESNQPKILEESRTMLILLMRLPQQKLQSVLFESSEKALDQLDYAFHTIPQDFLDSSKTQIDEFRCRVNRMIMHHQKLTNQARMNKLRQALGCLKKIKKNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TFY3</accession>
    <name>A0A8S1TFY3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0200392</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1 10">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Endoplasmic reticulum membrane</location>
            <topology evidence="2 10">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the OST1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TFY3"/>
    <dbReference type="OrthoDB" id="310030at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007676">
        <property type="entry name" value="Ribophorin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21049:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21049">
        <property type="entry name" value="RIBOPHORIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04597">
        <property type="entry name" value="Ribophorin_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="420"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8150653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="449" mass="52328" checksum="886BB4A6A190FB45" modified="2022-10-12" version="1">MLLFIFIALASSLRIPKNFFISNIERTVQLTDRYVNIQSKITFQNDGVIPIERIYYTIHNALSDRLYHVNIEESNSKKIIEDVDVKYKQNATVYEFTLKRSIEPSSNLEVELNEYYYKRFKPLPKEIGVEDEQLVQFMDSVYFFTPYLVKNQVSYYETPKIISYSLKSATQRNQLLEFGPFQDIKPFSTQIHTIHLENNTPMTVFTKASKIIEVSHWGNILIEENYSLENQGAKLKGEFGRVNFNKYNPNVGKHSLKQLSAQLPYDSWGVYYRDEIGNISTSNAAKGKLNGENQALIQLRPRFAIFGGWRSNFTLGYNLPTKKYLEQDGNNFRLTLNFSFSVREMVSDKYNFQISLPEGAYDIQVDLPLQVERNDTLLFSYFDTVGRPTIILDKTTTSQFDDKQIIITYKFSGISILREPLMIFGTFLFAFLLIIYLRRLDLQTLKLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TGS4</accession>
    <name>A0A8S1TGS4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0210369</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TGS4"/>
    <dbReference type="OrthoDB" id="284650at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8151470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="126" mass="15401" checksum="6A307FA005D3EE03" modified="2022-10-12" version="1">MVDQLKDLLYLIKQVDFEASNFEELYQTDAEYFSETVYRDIKHLEQQHYDLIYSSSLRFLSNVLQCLQFNFEIDIYEFDIRDWAQQGLDHILKFSGLRRSFRQPISCCQRYCKCISNLFKKNRQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1THB0</accession>
    <name>A0A8S1THB0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0230132</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1THB0"/>
    <dbReference type="OrthoDB" id="308569at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8152595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="309" mass="37082" checksum="93FA5F1A6DD7F6E8" modified="2022-10-12" version="1">MRDVLQKMMFHNFQFILIIIYICLMNTIVLGIPNEHQRKVIEDLKRDDKLYDFHPNEKLNKKMKIGPYPQDQIDCNQVSICKYCKSNEAYLINTKNIHLIGLHFGYKQEILDLFQSLKFIDEDSELWFAFSEKSICIEKSQLQIDISLYSIFNQYQIQQNSFEVFPQLFSIQDNIKLNNIKMMTKFDRDNRNDWNYEIISLNYTGSCDSDILFCEEFYQEPSNVHLRVKGKLKIKEEIGFEIIGYKNNKQINIEDIVLPNLIFDQLIKQFFGDRFIEQIEYDMNKQYKIEVIESLKDHKLELELMIEEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TJU0</accession>
    <name>A0A8S1TJU0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Tyrosyl-DNA phosphodiesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0220310</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tyrosyl-DNA phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8152163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TJU0"/>
    <dbReference type="OrthoDB" id="47785at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017005">
        <property type="term" value="F:3'-tyrosyl-DNA phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09122">
        <property type="entry name" value="PLDc_Tdp1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09123">
        <property type="entry name" value="PLDc_Tdp1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000071">
        <property type="entry name" value="Tyrosyl-DNA phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000081">
        <property type="entry name" value="Tyrosyl-DNA phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010347">
        <property type="entry name" value="Tdp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12415">
        <property type="entry name" value="TYROSYL-DNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12415:SF0">
        <property type="entry name" value="TYROSYL-DNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06087">
        <property type="entry name" value="Tyr-DNA_phospho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="429"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="431"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="11">
        <location>
            <position position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR610347-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR610347-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR610347-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8152163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="532" mass="62838" checksum="C79548E94E12908C" modified="2022-10-12" version="1">MKKRTLQDLSEEQDQINSSYIEINSVQKELYLENQKRLKEKQKEKEQIVVIEDDEIKQQIEENEVQLSFPIGTFCLNCLTQYPTDEKQQHHLPFINILYENAYIDVKTQKPKKTLESALMTCFGFEDELLEPIVKSGVQLFIVYNNDNQNKKLEVIEKYNNYPNWMVIKPSKLSSSMYGGAFHPKIWILKFPKFIRIVIGSQNLHVGDWTIWSQAMWIQDFKLGKSELDQTSQEFKTMLREFLYEILPTSHKFEDLLKIKYDDYDFKDVNIRLITSIPGRFVGNQLYKYGMMRLQSVLYQELCNNKMEIPKQVCVTYQTTSIGQLDNNYIDFALQCCTGRVYKQPLTGEQNNKKVNQMILNQQEEEQSKLKLIYPTADYIENQTHGGVDFANPLHFKKQLYENPKFPKHLFYKYQGSDHYYWHTGNIPHLKVMIITGLDEDINDYTSIYIGSHNFSQGAWGKMEKNATQLYIANTELGVLYPPKKNSAQLKQQIIEQLSFKFPPLKYEKDDVPWIVEAYYEKLYKEHQQQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TXN1</accession>
    <name>A0A8S1TXN1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0300263</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000030">
        <property type="protein sequence ID" value="CAD8157625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TXN1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8157625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="110" mass="12570" checksum="822947D21F1F2C81" modified="2022-10-12" version="1">MNTAAFNQPNPNTRQTQNPAPTFQPLPTTSTLNKGSFPNQNQNMIGNHNMIGTPNVMGTIANLQLQNPIQPIKNQDNNQKMELVEQLNNVVREAKERRAKIIDFYEKYKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U1E3</accession>
    <name>A0A8S1U1E3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">LITAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0310226</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDIP1/LITAF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U1E3"/>
    <dbReference type="OrthoDB" id="310679at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006629">
        <property type="entry name" value="LITAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037519">
        <property type="entry name" value="LITAF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292:SF6">
        <property type="entry name" value="FI16602P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292">
        <property type="entry name" value="LIPOPOLYSACCHARIDE-INDUCED TUMOR NECROSIS FACTOR-ALPHA FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10601">
        <property type="entry name" value="zf-LITAF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00714">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51837">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="LITAF" evidence="7">
        <location>
            <begin position="103"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8158214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="189" mass="21634" checksum="CE173C9618ABFCE1" modified="2022-10-12" version="1">MDQENAKLAQDYQVQNNIPLLQDQQQENNQYYSPYNNQVQYVQQQVAQQQQLSNSYQGINLQGDQYQVNQQLQQQNPQLQQIIQPYQPNTNQQQITGIVIQTPQIYNLIRNASGFRTPTLISCPYCNKNAVTNVHFKSGDDTFCMAVLLCLCFGLLCLIPLLSSDCKDAYHRCSHCGRTVGFTPYQACQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U799</accession>
    <name>A0A8S1U799_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0340335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8160434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U799"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8160434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="64" mass="7434" checksum="398CC8C965562750" modified="2022-10-12" version="1">MPKRSTHYLLPLQPLHLVIILICCINLLSLVIRFYCNCQLHHVIEIMKNINFIILWSFSSIGVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U975</accession>
    <name>A0A8S1U975_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0360324</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U975"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8161711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="191" mass="21550" checksum="1D226138084229E4" modified="2022-10-12" version="1">MTSISVGGVILSDCLSQSEKVQNFYCSENWKCYRIRQFGMELGAGGGPIGVVIGGIIGGFIGEVSGNLLGKAIDHFTQFNLQIDFNQNKKSKVEDGYLAYLGTPPQDKLEILQFQQHKLTLIWLFQDIGIQFNEYKEKDPDDSCKSITFKLLAITEEYVNDDEILQQFFSHETNIIDLVKVKISLENIKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UAV7</accession>
    <name>A0A8S1UAV7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0370139</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CEP135/TSGA10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8161978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10254663at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051877">
        <property type="entry name" value="Centriole_BasalBody_StrucProt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20544">
        <property type="entry name" value="CENTROSOMAL PROTEIN CEP135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20544:SF0">
        <property type="entry name" value="NUCLEOPROTEIN TPR_MLP1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="259"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1219"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="31"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="624"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1068"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1187"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1225"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1236"/>
            <end position="1248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8161978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1296" mass="155956" checksum="D1FDE01C342FEA39" modified="2022-10-12" version="1">MDQERKFLLLKKRLDALHYCQPLGIDSVPLVERLLNDLVKTTEGFQQLKKQLEETKINAAKFEQLLLPLKKENQRIVNENNQLHMTMIQIKEECDTKENKWRSQVRMLESERNDWKFILSQKNNAEKKLQEELFKKNNENNLSSSNQPDKTLKLIDQIQDLEKKLLQVQQDSRENDNQKVLLEEKCKHLEFQIENREKEILRLKSKLDIQGTNIEKLAQEHLISETQQKAQRTQTQLDLLTEENTKLNDELTNLRKELSRYRKKPSQPQQQSPNKSDKKNELNQIKQRLSSIEEEKEKNQEQFKKILNQLEQENQQLVEKVQQLYQQNEQLQLKAQNAEQVVQAYQTDKMVFSKAVNNLKNDKESTVQTLRNLEEDNQKLKYQLNEMDDQLQIQRSEYQQLQREKELYDRNYKKLNDEYEREKDFVQQLRQDKRDLERELDQEKQIIRELKIEFERMDVSKQNLDNLLQKFKQEQKNQQQSHQQNPNQGQQLIIDQLRKENDLLTKEVQTTENLIKQIRKDLGEKDRDNYELKQQKRLLNDQINELSQKNANLDSQIKLKLQEIAQLEQEVEQYKREQSKMRLNQQNILEQKQKIENDTVGELKLLQQQNNSLKLQLSDLGIFNSQLEEKLKIAQELLNDQRLENDKLQMNLRQQSDETFKYQQNLLRNKDKEMDYYNLTNEIERLRDQERKILIEKERFQSENLKLESVINQQSKQIEFLNKLKEEAMKEARELRQMINGLKTNDHEIKNTSIKLSEKVNNYEMTLQDINEQLQKEVQRSNKLYNELTESQSQISSLKLQKTDSENQIRQIKLLCESLEKQKEELLQRLQQKNSTKIEDERQVLQLRELNENLEDKVNQISEQNVILKRSLEQLDLERDEVQNQLDEVRETNISLQQQIKQMNAEIQKQQSKLSEVSTKGERGYEAAQQLEEKLRDQIYKNQKLQNENENLKYTLQIKEKEFHEMRSDIQILSKDNQSLNNQLVKSVQEKELQSQQIQFLQQQEIRLKEQIKMFDMEMHDLQLNYSEVCAENQRLTVSIQQISLQHEEVLQRGVELEKQEDFFKVQMQNASKREDQYQIQLQQYEREIERLNRKSEEYERQIRDLIKDRDQLGKEYHSQRKISTGFQAAQEDFNRQIAQLEREKQYIENANRDLQKQVDLKDAQLSWEQKRVNEMEQVIIQERQLQMNTLQEIQQLKDQNEQLLQELEKAYQPQKYSQNYKNESSYVSQQSQQSGIKDNSQLDQSSGWERLKQESENFKKTLSQAAISSQNLKQKMKSLQREYEIQQNPQQQRYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UB99</accession>
    <name>A0A8S1UB99_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0350269</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="8061355at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03263">
        <property type="entry name" value="ABC_subfamily_A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000335">
        <property type="entry name" value="ATP binding cassette subfamily A member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001253">
        <property type="entry name" value="ATP-binding cassette protein subfamily A, member 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF36">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY A MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="92"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="171"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="713"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="753"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="797"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="835"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="863"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="902"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="949"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="319"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="1007"/>
            <end position="1239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8160982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1327" mass="152368" checksum="E76DDB0C7798C086" modified="2022-10-12" version="1">MNSQQQIISLIKKNLLISYRNREIFIEALLPIIVSLMLTFREYLSEMKQLMPLLYSLATASTQRSMLIKLVEEKSKRYKELQKIMGMNEKSYLIGWVLTGYIRVIIAVIIFELSLFLMFPIFGIKWDDQFNESFISLTWPYFLFSIASMNQIYLFSSLFNEVKIAGEIQSFFQIACVFFIYFTFVESAANSFSFYFFLSIFSPQCSVAFTYIASMKPGIPGDILSANLFPSEQIMDIYSTKYEGAQMAVQAIIYFLLFLYFEQVIPNEYGVAKEPLFFLKRNKVQIKENDLFAQLKSEDNDVSSAIYHEEIKYSVPPSIIIKNLQKKFGTLKAVDNISLSLYESEIFCLLGHNGAGKTTAISVLTGMISKTSGLVSMYGMNLDTQLPKIRQSLGLCTQKDCLYEDLTVEENLKYISSIKGKVDKYEIFEILKKTDLIGERHLQVKVLSGGSKRKLSLGMSLVGNSKIIFLDEPTSGMDAYSRRQIWSILERIRQDQRTIILTTHHLDEAEILANRIGIMSKGQLLAVGTSNFIKKKFGEGYNLKITFSDVQLRNQIYEKVNKYVPECFLETEHSNENQYVFNIPFTSKNTLGQLFQELEQMSVQIGLSMKTLEDAFVKIGMEEEQAQLTFRRQSEIQIAKQSLGIPASDDDNERDNQEFLSYPVEIKDKDTQNEDIKSIPKCLSNDPQYSFLNQILAIFLRRYYTVVRTTTNYFSIIIPMIAFINGLVVVAYVEFQDEIFKILPEYVLNHFKINLLASCCVIAFCFNATIYITQPVLEKEYFLKQSLQGMGLRNLTYWLGTFFFDLIIFFLNLVLFLIVSALLDLNIVFDNITRSLSCFLVFGPSQILFAYIMGFVFNKLESALKLFTIFCFFILFCLPNILVAFTFFFYKELEQPVFLKNVLYVFEILFSIISPFYAFFSAYLFTANNYLEVESFFEAPYLLTTTESYLLIILGQIVVFTGILFFLENKKSFINHNQIQQSVNENIEEEVQNERYRVLQADNNDPIKSKFLEKQYTKGKPTLQQLTFSVKKGEILGIIGPNGAGKSTLINIFSGINTPTAGQALLNDYEPKQQIMSIMQHVGVCPQFDCIWENLTLVEHLQLFGRIKGLQGKELSAAVSYFIKTMQLDLFLNTKAGQLSGGNKRKLCVADALIGGSDITFFDEPSSGVDPISRRFLFNTLKRNIQLRACSAIMTTHTIEEAESLSDRIGILIAGQFKCLGSPQDLRQKYGSGYQISVKYIYPQVTILIQNQFSYAKQIEEKREGYFMYSLPKEGFSFYRSFNFFQSLQQEGHIEDFQIEENSLESVFIHFSKIQQEINEKDGIAID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UBV6</accession>
    <name>A0A8S1UBV6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0380211</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UBV6"/>
    <dbReference type="OrthoDB" id="10252009at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000134">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF519">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE MPK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="5">
        <location>
            <begin position="19"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="6">
        <location>
            <begin position="78"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8162731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="159" mass="18379" checksum="0DB4D1D042531312" modified="2022-10-12" version="1">MQVSELLEYVPESISLILEPTQNKGALYLGNITSLFKSTQKYQIKAAISICEDLDLKEVQLEQHLRIVLNDLDNSNIKQYFDQTNQFIQSNLNKGNLLVHCMAGVSRSAAIVIAYLMWSQKMTFQNALIYVTKKREQVYPNKGFRQQLIQYEEELKTVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UBZ4</accession>
    <name>A0A8S1UBZ4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0360209</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UBZ4"/>
    <dbReference type="OrthoDB" id="432483at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000876">
        <property type="entry name" value="AGC family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000210">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="42"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8161483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="366" mass="42330" checksum="4412D657AE593E82" modified="2022-10-12" version="1">MGNCFNQDENAKTSLQSLKKVDQLQSQSLQDLDINKITLKDFLSQGQIGRGQFGKVFKVKMKNSSQEYAMKVIKKSEIIQFGLVEHTMLEKSVLIRSKNPFVVKLKYSFQTDQKLYLVMELVNGGQLSKILSKYHQNRFTQIQAQFCAAEIILGLEYIHETLKVLYRDLKPDNILVTQDGHLKLTDFGLSKQYDTQDMKFFTFVGTPEYIAPEILLKSGHNSSVDWWSLGILLYEMLVGYTPFRDQSNNLRVIENKIIQSEILFPDFVSNEAKDIISQLLNKDPNNRLGSRSVDQIKKHIFFSQINWDDIYYLRTKSPILDGLTKILQQKQTENKGVPKKIFETPQSQIRQTNGQFEGFSVNHDEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UGU6</accession>
    <name>A0A8S1UGU6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0400202</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8164060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UGU6"/>
    <dbReference type="OrthoDB" id="285716at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8164060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="200" mass="23894" checksum="CA45168529285278" modified="2022-10-12" version="1">MYSVSQISKSDPTRYGDQFKPTARKMTHRLLSHCDNELTLKKMDFALSGSGKQIPDYNIKSTQIETKQIEPFKIKDFIPQINPTIKGELSRLNKNNFLDFLLIQKAGKLGDQKFFDLLIQQYNFDPFKKTLKMHDNKQQIQYLQKRDEREEQIALQIKQFYKHMQHKRRGTINEQPLDRMIIEKNNYLIQKLPRFRAKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UGZ7</accession>
    <name>A0A8S1UGZ7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein kinase domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0400311</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8164278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UGZ7"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000537">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="56"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="10">
        <location>
            <begin position="312"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8164278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="371" mass="42860" checksum="9ADFEA73DB4172F5" modified="2022-10-12" version="1">MGMCQNKPAEYNSKIPNVTSEILDEEDVEDKIGETTYLPIEEENGNLKTKPTLSDFELLKVIGRGSFGKVLLVKRIADQQLYAVKILRKKLLQKKKQQEQALQERKIMSLINSSFSVKLHFAFQSPSRLYMVMDFMIGGELFLHLRKRQKFSEEWTQFYAAELLIALDILHQQKIIYRDLKPENILLDKDGHIVLTDFGLSKLGYERNEITYSFCGTPEYVAPEILYQKGHSFVVDFYSYGALIYEMLSGAPPFYSKNKREMLKNRCEKPLEMKSCFSLQAQSLLKGLLTKDPGFRLGSNGIQEIKKHNFFSGIDWELVEQRKLQPPIVPRIKNLEDLSNFAPAFLQQPLIETPDSQQNVQFEGFTYQQGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UME8</accession>
    <name>A0A8S1UME8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0430019</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8165574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UME8"/>
    <dbReference type="OrthoDB" id="309893at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8165574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="264" mass="31546" checksum="66AA17B725B19661" modified="2022-10-12" version="1">MIIANETQLLSSQSQQNIEFQVQNTVNNQIHIYRQLRANDSQIQNNSFHYDDLDPQIRRKCLNLRNKIHFENQKDELEFEDIIKQWNQSLQQNTRLNQMVTEQLNQQYQNNQYQTDFPVSKIHILDYEENQPITSFSLFTSKDLIIENLILQDIPLEKSTQTDYPNQKDDKFTQTDLIDIDKDDTENVVEFFNSSFYKDLKNKQFLIDAQLISLIQTIFLMHNERKIQINEPIKQQLDLLMKKIKKKDQINYNTIVIIKDSVDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UN49</accession>
    <name>A0A8S1UN49_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0420058</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UN49"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8165132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="60" mass="7080" checksum="41F06A41C8CD7862" modified="2022-10-12" version="1">MQANKHEEQTAQKISRAIQLINDPDLQMQFKPNKKLIILQKIAEEKIRRNVIILKIICNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UP47</accession>
    <name>A0A8S1UP47_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0400290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8164236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UP47"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="368"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="407"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8164236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="484" mass="57703" checksum="C993728E08F8413D" modified="2022-10-12" version="1">MDPLQIYKGKTIVIEHIVNDITYTILFYIYGYLQITWYTMYAEISFYVSDQNKKENIHKNNQWICQNYKNIVKFRLFFIFLVQIAVSIFTGLRLEIDFPDFAILCYSYLLINFIIFIFEFALYGIKLQNCIRYQLTQLYYEKKQKIMNLITLKEELQKQLEQKQVDGQFQQIDQIKKIDQFSSEKSSRRQSDDLSQVIVPSQKDRQKKKVTFCKNNTSLMFYQLESHHNIKITKDINEVIPEQPENFEREFYGLENNNKSFCLEESIKQIDWIGDKKDQEIIEKVRQKKLLIVREAKEKIKGEHEQIIISQKSNIKKQIDLSNLSDQSQDQTQYTQIVQQIDVFEQQKYLEEQQLSQKSNSLEADKNILYQIMLLIFLGITYEVMFGIMCIIALVSEAIYSTPTGTIVYLYVSSTLQFLSLFNVLKLFKDFRSQQTKSFIWIQKIGSKKNDLDLNYSFIIPKEYKQDDDMLRKLESRINLITLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UR53</accession>
    <name>A0A8S1UR53_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Spindle assembly abnormal protein 6 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0460002</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8166683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UR53"/>
    <dbReference type="OrthoDB" id="284211at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10142">
        <property type="entry name" value="HD_SAS6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032396">
        <property type="entry name" value="SAS-6_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34230">
        <property type="entry name" value="ASSEMBLY ABNORMAL PROTEIN 6, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34230:SF2">
        <property type="entry name" value="SPINDLE ASSEMBLY ABNORMAL PROTEIN 6 N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16531">
        <property type="entry name" value="SAS-6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spindle assembly abnormal protein 6 N-terminal" evidence="2">
        <location>
            <begin position="28"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="165"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8166683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="209" mass="24678" checksum="8400CD2AC362C933" modified="2022-10-12" version="1">MLKNTPSKEILNYQETQEETDSGGYTLIYEQELVSQILVEDGDQQQTQETLNVRVLIFGNDQRLERIKIELSCENDLFFHFIHDINEEAFQKIKDEQQLTASFIDYPAICIKCLDKAHKDPNKYSAVLRITQEGNAFIEIIQKTEYKNVELIQFQFFSLPEESIRMTITKKYQKIQQKLQQTENKLQDINDVVKVKNPQLLLQIQRMNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UR65</accession>
    <name>A0A8S1UR65_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0460012</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8166693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UR65"/>
    <dbReference type="OrthoDB" id="423343at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8166693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="337" mass="38958" checksum="86F64AC742CB7AF2" modified="2022-10-12" version="1">MGQCSCQESIPITNEVSPINLFQKPIIITEEQVNINQATHSLEVDKAFSQAKTKQMEDSEELEFHRPNKAEAWETSKLFETISEKVRELQNELPPLDLEEREGYFYGVYELKNGSIYQGTWIEGLREGKGAQIMKNGALYEGYFVKGKSKGQGRMIYPDGDYYVGEWLDDQLHGYGEYYHADGTKYKGNWEFDIQHGYGEEYFKDSSIFKGQFQKGKKFGEGLFIFPDGSQYEGQFQSNYFSGKGKYTWPNKKQYIGQWEQSKMNGQGIMIWQDGTRYEGSYLEDKKHGFGTITWPDSRCYSGQWLNGKMHGIGEYTSSTGKARKGEWLNGKRLKWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UTK7</accession>
    <name>A0A8S1UTK7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sperm-tail PG-rich repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0490191</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UTK7"/>
    <dbReference type="OrthoDB" id="300759at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051291">
        <property type="entry name" value="CIMAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580:SF28">
        <property type="entry name" value="BOREALIN N-TERMINAL DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580">
        <property type="entry name" value="SHIPPO-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8168588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="372" mass="43062" checksum="8C4C4226633EA075" modified="2022-10-12" version="1">MNEQKDQIDQENEKEQDQQEKDSRVDDEQKEFYSQDTPQQQQQQIEDQPEIRQQIVQHNSKRVDSQEEIKTTTQRKELNKRIETEKQYGQKTTSERTFKTLEQIYSKRNTQSLLHLRTSEQFDTKIIHDQINRSHSKQQYTFSKAERFSKAKESYCSIQFYDSQIQTQLNKRAAALGYGNKSDFTKKDKYIPGPTDYNIKLTLNPGVKFGYGRDETMIKGIHGRPNKNPAPNLYQVKDIVTTYKYSMGERTSAKHIYLQSTTPAPGRYDVGGLNAKGNYFFGRFKSSGAPVISPSTTIAKRLKRIPGPGTYDPPGDIGDLRTYLPSQYSTRSGFRFGFQERQTQNIKNKQFPGPGTYQLPSEFGDYEYPPLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UTN3</accession>
    <name>A0A8S1UTN3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0480029</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000048">
        <property type="protein sequence ID" value="CAD8167844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UTN3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8167844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="64" mass="7294" checksum="0E04AB7B15C4BCE1" modified="2022-10-12" version="1">MDQAKILIPEVKLKTLLIKALISKKNNIIPSEGQNSFFRVGETLSLRSQFSKSDKIKIQFDKES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UVU0</accession>
    <name>A0A8S1UVU0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nuclear nucleic acid-binding protein C1D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0490046</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Plays a role in the recruitment of the exosome to pre-rRNA to mediate the 3'-5' end processing of the 5.8S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer and homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the C1D family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UVU0"/>
    <dbReference type="OrthoDB" id="10261072at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000178">
        <property type="term" value="C:exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011082">
        <property type="entry name" value="Exosome-assoc_fac/DNA_repair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007146">
        <property type="entry name" value="Sas10/Utp3/C1D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15341:SF3">
        <property type="entry name" value="NUCLEAR NUCLEIC ACID-BINDING PROTEIN C1D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15341">
        <property type="entry name" value="SUN-COR STEROID HORMONE RECEPTOR CO-REPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04000">
        <property type="entry name" value="Sas10_Utp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 6" id="KW-0698">rRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8168357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="187" mass="22176" checksum="8C43F0033E7E0DE6" modified="2022-10-12" version="1">MSIQTTLQSTQEELTKLQKLIQELSEKGNLDEITQGMTQKEQIEFNLNLAYTLSSIYYSYLKLNSVETSTHPIMNELTRIQEAFQKYMPSQIKQNDQKQMSLDKEAAKRLIQPNLKTLDDNSNYQHKQKKLIEEDRNNTTRFEQPMDIWKNDKDQQDQNKQKLIRGNLIPQTGHLKWKDQIEKLMKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UXG4</accession>
    <name>A0A8S1UXG4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutamine synthetase</fullName>
            <ecNumber evidence="3">6.3.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0490009</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-glutamate + NH4(+) + ATP = L-glutamine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16169"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glutamine synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UXG4"/>
    <dbReference type="OrthoDB" id="1936100at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000011">
        <property type="entry name" value="Glutamine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027303">
        <property type="entry name" value="Gln_synth_gly_rich_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050292">
        <property type="entry name" value="Glutamine_Synthetase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20852">
        <property type="entry name" value="GLUTAMINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20852:SF57">
        <property type="entry name" value="GLUTAMINE SYNTHETASE 2 CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03951">
        <property type="entry name" value="Gln-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00181">
        <property type="entry name" value="GLNA_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51986">
        <property type="entry name" value="GS_BETA_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GS beta-grasp" evidence="10">
        <location>
            <begin position="25"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="GS catalytic" evidence="11">
        <location>
            <begin position="113"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8168269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="398" mass="45859" checksum="23C2967CAED6A9D1" modified="2022-10-12" version="1">MTETIENLRDFNYYQLTKDVNNSVVLAEYIWIDGTGESLRSKTKVYQTQIKSLEDLEWWTYDGSSTDQAVTRFSEIYLKPVRVVKDPFRGDPHILVLCETYLPDKKTPARYNFRWIANLIMEKAKDHKPWFGIEQEYFLLKRTGTTHLWPLGWPTGGFPYPQGRYYCSIGERNNFGRALAEAHLRACLNAGLKIAGLNAEVAPSQWEFQIGIAEGIEIGDHMWLARYILERIGEEFGIDINYDPKPILGDWNGSGAHCNYSTVTTRSEGGYRYIVDKLMPILKENHLEMIKLYGQKNELRLTGRHETGKYDQFTWGDGSRGCSVRVPIITKEQGQGYFEDRRPAANIDPYLVSAALVDVTCLNSEHLKQLNSVFEDSLKPLGQQIFQQQKEQFQQQNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V443</accession>
    <name>A0A8S1V443_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD-40 repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0560108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000056">
        <property type="protein sequence ID" value="CAD8172140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V443"/>
    <dbReference type="OrthoDB" id="163438at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25173">
        <property type="entry name" value="Beta-prop_WDR3_1st"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="215"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="257"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="299"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="341"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="383"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="425"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="467"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="509"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="551"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="593"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="635"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="677"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="719"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="761"/>
            <end position="802"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8172140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="888" mass="99139" checksum="C4A0E061E9CB83D0" modified="2022-10-12" version="1">MQCPFLEKKALADIFTQIKDVDKQIFGLILEIFQKEKVEDCIEYLSDNENQRHLEQQILQIGNLTEVERLSTGSSDMKQITDVLKKIKDHAYNNKDYSSEENQEQTQNLINSIKNDKYIIEFLLFLVYLTTIDNHLIQCGSNSLHILVQMKIDIRNLHFENIKIQNTSLQGANFVRCNLSGSQFNNVNISGINLNGALLMNCKWTNLRIHELNQLNGHTNYVNSVCFSPDGNTLASGSWDKTILLWDIKTGQQKSKLDGHSGHVMSVCFSPDGNILASCSYDKSIRLWDVKIGQQKYTLNDQTRFVTQLCFSPDGNILASNNDINSIFLWDIKTKKKMAIFNGHTSYVMSVCFSPDGNTLASGSYDKSIRLWDIKTGYSKTELHGHTSGILSICFSPNGDTLASGSYDKSILLWDVKAGQQKVKLVGHSHYVMSVCFSPLGHTLASGSYDNYICLWDVEAGQQKEKLVGHSHYVMSVCFSPSGLTLASGSYDNSIRLWDIQIGQQKVQLDGHSDFIRSICFSPDGNTIASGSDDKSICLWDVKTGKQKIKLDGHTSYFMSVCFSPDGNTLASGSYDKSILLWDVKTGKQKIKLDGHSDVIRSVCFCPDGNTLASGSSDNSISLWNVKTGQQKAKLDGHTSFVMSVCFSPDGNTLASGSYDNSIRLWDINIGQQKVKLDGHSDFIRSVCFSPDGNRLASGSNDKFIRLWDVNTGQQKAKLEGHTSSVMSACFSPDANTLVSGSQDNSIRLWDVKTGQQKAKLDGHTSFVMSVCFSPDGNTLASGSYDNSIRLWDVKTRQEMHLRDNQYQDIFTQFQLTISKKNLQSDIIIQHNLVVNTNITILLISQQKIFQAQGSLIFKGEFINQQGIDLKTLFKQKGSCFMVSLSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V5X2</accession>
    <name>A0A8S1V5X2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0560129</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000056">
        <property type="protein sequence ID" value="CAD8172174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V5X2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8172174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="120" mass="14156" checksum="CBCFE22167A6B1C2" modified="2022-10-12" version="1">MKISFANKANIVLNKIICLFLTDRISNYQNISFPIFDPVYFEYCKDTYQLVLLQFPIIILKIVQRDLNMEKNQQNIKSERIQLLQSEQSVSTLLLFLNIILTSFKIHQQEGLLLISRVQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V901</accession>
    <name>A0A8S1V901_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0590060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000059">
        <property type="protein sequence ID" value="CAD8173334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V901"/>
    <dbReference type="OrthoDB" id="10310953at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8173334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="152" mass="17721" checksum="DF4C60DC9A3E7A31" modified="2022-10-12" version="1">MSDDEHFSLHSFDPDNNIDNDIDQFYKKLSLQFYGENKKKKVSLLMSGSTKETSKPSRDTLPEVQKGFMYMTPLSTRPGSPRVGRIQVQKRATNQNFSGSPSKTRPLQSNKLLQLLQSPYNSKNTQKQAFIKKQIDMIRRMQLQELKEHYYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VCY6</accession>
    <name>A0A8S1VCY6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Srp40 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0610126</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VCY6"/>
    <dbReference type="OrthoDB" id="5599646at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007718">
        <property type="entry name" value="Srp40_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05022">
        <property type="entry name" value="SRP40_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Srp40 C-terminal" evidence="2">
        <location>
            <begin position="125"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8174481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="201" mass="23483" checksum="9B93697CC403A0B5" modified="2022-10-12" version="1">MQRDLTPQDVLPLIVEYLKSIGLKKSAKQIDSLYEHDETPMANKDLQKIIKYYVEGHSKLAAKFVAEAENDEQEEQEEETEQQKQEEKNNKQVTKVLSDKRTFTQMNKIPINEEVVDQKEKKRFFQKCDEAVFDKLPDHLRDNTFEAKKKFGEGDQYGEFGNDKLKFTRGDNFKKEKGKLKNRQFQGMGSINLNSINSIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VDC3</accession>
    <name>A0A8S1VDC3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">L-2-hydroxyglutarate dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="6">1.1.99.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0610102</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(S)-2-hydroxyglutarate + A = 2-oxoglutarate + AH2</text>
            <dbReference type="Rhea" id="RHEA:21252"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:16782"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="EC" id="1.1.99.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.99.2" evidence="6"/>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VDC3"/>
    <dbReference type="OrthoDB" id="498204at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047545">
        <property type="term" value="F:(S)-2-hydroxyglutarate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43104">
        <property type="entry name" value="L-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43104:SF2">
        <property type="entry name" value="L-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="8">
        <location>
            <begin position="5"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00036066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8174455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="422" mass="48309" checksum="B5AF68CE2DDEFE0B" modified="2022-10-12" version="1">MISFDIAVIGGGVVGGMLTRSLSARYPNHKIGLFEKESGLGLHTSTRNSAVLHAGFYYASNTVKASLCKEGNKTLTQYCLDHKVNIRKTGKFLVARNSAENERLAQIKKQGDINGVELELMEVDRALKIEKYLKVNDKNFQFLYSPTTSVADNKGLMKSIHEDLNQCKNVQVFTNHKYTDLIENKDQQTTFNVLKMSNEKQLVQAKYFVNAGGLYADVIAKQFNFCKKYVIWPFKGSYLINNRPIPEANAIIYPVPPLAGNYFLGIHTTITTDGHLKIGPSIFPALWREQYNWKDNFVFSEFSEILSLNVKNVISKDMKFYAQSLLNELKKINKTYMIKEAKKLTTQLDQIPENQLHEYFDRGKPGIRSQLFDTEKRKMEQDFIFEHDKNSFHLLNVASPGWTCSIPMSEYICDIIQKNNYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VHS7</accession>
    <name>A0A8S1VHS7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD-40 repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0650246</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000065">
        <property type="protein sequence ID" value="CAD8176764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VHS7"/>
    <dbReference type="OrthoDB" id="10264588at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="189"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="231"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="273"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="315"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="357"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="399"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="441"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8176764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="608" mass="68882" checksum="980B522914A80BE8" modified="2022-10-12" version="1">MDQTFQDQEALADIYTQVKDVDEQLFGVLLEIIRKEKVYDYIRYLLDNGNQKHLEEQILQQRENLKQAYNEMKLYDFRKNIQQITDVLKKIKDHKFNKKDYSNEENQESIKDLIKRIRYNKKIIEFLYFLVQLTSLDNTLIQCVGANFFRCNLSGSEFNNVNISGMNMNRALLLNCKWNNLRIQELNQLKGHGGFVRSVCFSLDGNTVASGQDDNSIRLWDIKTGQQKAKLHGHTNGINSVCFSPDGIKLASSSFDNFIRLWDVKTRKKMAKLIGHTNFVNSVCFSPDGNTLASGSSDMSICLWDVKTGQQKAQIDGHTSGVLSVCFSPDGNTLASGSSDKSICLWDVKTGQQKAQIDGHTSGVFSVCFSPDGNTLASCSKDLSIRLWDVKTGQQKAQLGVHQLSVNSVCFSPDGNTLASGSWDKSIRLWNVKTRSQKAKLDGHDEPVTSVCFSLDGNTLASGSWDKSIRLWDLKIGQEDKSLDKHNKDVLIQSKISFQKNSPLSEDISIYTTKVLISQKAIFQAQGALILKGEFINKSGIDLKKLLKKKGSCFLKSLQYKYSYNKFLKFFNQVFISSTLHLHYIRQKISIYFSKVFLSFQKNVGNIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VJX2</accession>
    <name>A0A8S1VJX2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0660054</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000066">
        <property type="protein sequence ID" value="CAD8176893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VJX2"/>
    <dbReference type="OrthoDB" id="305154at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8176893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="118" mass="13809" checksum="254C7D0DF51A1B8C" modified="2022-10-12" version="1">MTQVASIQNLLIDDNNSTLNQQKGLGIAKKIKKKEKGCFCKQQKQKKIYKYISPDESSYECDGWDEEDQFNRQSENRRQNKDITDLKCVAWNLELFIKNAKQQLAYLEGTMQQNLARN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VND5</accession>
    <name>A0A8S1VND5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0640253</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8176266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VND5"/>
    <dbReference type="OrthoDB" id="288581at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8176266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="154" mass="18248" checksum="488EEC822EF82815" modified="2022-10-12" version="1">MDCQRTYLNVNQRKKNNNYPLYRLDAIFERCEDGSSIGSNLQKRIGIDIFEDANQLKKVQTKLDITNLKQNFFNNSQNISNQFFEQKKNLQQNLEIEWTNSNKVLQTNFINTEDINILEVDEINYLELNASPKIKRFKTKYSQKISKAIKKFIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VS87</accession>
    <name>A0A8S1VS87_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0710029</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000071">
        <property type="protein sequence ID" value="CAD8179005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VS87"/>
    <dbReference type="OrthoDB" id="10252009at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000118">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF519">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE MPK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="5">
        <location>
            <begin position="17"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="6">
        <location>
            <begin position="62"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8179005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="238" mass="27115" checksum="2F1935E7FD6E3B1A" modified="2022-10-12" version="1">MLFRGINNYKSMNIILDQTESQGALWLGDYTAAINQQLLKQKNIKTVLTVASGLNVKYPPNSDIVHKVYNILDIESCNIKRIWGDTYQQIDEGLQKGSVLVHCAAGVSRSAATVIAYLMRKQAMSFQEAFQFVRLKRSVVCPNFGFQRQLKQFERELQNGNGKNDTEITQQQQLTQQQQQLIKPIMQQQQLSNLTIQPHSISISEKKTMVRRNQLTPASKFPKPAYLSNVKQAQSLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VSU5</accession>
    <name>A0A8S1VSU5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0740047</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000074">
        <property type="protein sequence ID" value="CAD8180320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VSU5"/>
    <dbReference type="OrthoDB" id="289076at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8180320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="386" mass="46302" checksum="939BFD85A358DDD5" modified="2022-10-12" version="1">MKIYKSFAFDQNIPQTDYEKYINPDDLLNESFEELKGEFKNYRTKKGKDIIKQEMEMRKQLQQKKKKNQSGLIVQQKKHEDQDNQKDMLGNIGLLIRQQERMCKQIKKEVQSPNFGQDYTTMKQKIQKTDVTKQPIFSQEDFAKLIQREDEDELIQTQTRTMHKPREGFKSRFIEKQIPMYKNMEQSSEIDSKKQMDSKILTKSNKTIKQFGLQQSRESPSNAMFEGEAKRISKSYLYKTWLKGEEAQNKKFKKPRRTESYHKAAAQREEIKSQLKERAQVAFKRYESMGKTWEKFGVFSQDILDFRMQTLKEAKIQRLLLEQQEHDLKKIEQNEQRDPDMLTRLVKVITKPNSDNKEEVKTNFEYHEQRLKDMRTRTRQIQRAGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VWI0</accession>
    <name>A0A8S1VWI0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0750158</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000075">
        <property type="protein sequence ID" value="CAD8181017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VWI0"/>
    <dbReference type="OrthoDB" id="342730at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="311"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="68"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8181017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="417" mass="50480" checksum="7B94D665973F1196" modified="2022-10-12" version="1">MSSKDKQSVKSDKSKDKKKKSTKLDKKDSKILPPPDNDQQQLIARVISRSGDKESKNLANCDDDPQMQTFNNNGNFQTNNSLQQHNQFENYPTVNKNNNQFRTNKFMNDQNTQQYQDHQYSKSPLQYQDQYYSPSYTQPYYPNQQFTQQATFDYRYERLKEQVNQSLLPKRQEILEQVQKIDNRIDEIKYQSQKIENITREECEYIIDKLKSAESQKLQMLYHDKNELLRDIDQIETLQQRISQLQEFNSQKNLDLFENIERLSRKRIKKQIDIYPDDLPQDLIQFRQLQQQNQMLQKLMDFKNEIIWKLFSDSQIENKRIREELEKQANQEFQEWYNIVDQYQQELNRYKLQCTFCGINFDYQLINSDCNQNLQDAKIQFDCEQIPPEQFIGTRRHFFSKNRQMQQSQIQMEDNKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W1D5</accession>
    <name>A0A8S1W1D5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0800001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8182951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W1D5"/>
    <dbReference type="OrthoDB" id="2335338at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050498">
        <property type="entry name" value="Ycf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE SPINDLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="82"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="150"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="184"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="218"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="257"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="291"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="359"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8182951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="416" mass="49329" checksum="B4C47274145318E0" modified="2022-10-12" version="1">MSNNSLNENENAGAIAHYSRSQEFKITYRTEEALLEINNALNLCPQFAEAYTLRSGLYEQLKLYDKALEDINYSIFINNNNAESYYQRATIYRRKKNFDLALQDCQRCVLINPNFAIGYSRMGTIMGDLKQKNSELSYYNQAIDKDENCYYAFMCRGLYYFDEQMFNDALRNFNQAIKINPKYALAYYNRAILYNDIGEKDKALSDYNQAIQLDPKYVNAYNNRGNLYDDIGEKDKALSDYSQAIQLDPNCAMTYNNRAYTLRSELYEKMKLYDKALEDVNQSLSINNNNAESYYQRGTIYWRKQLFDLALQDCMKCVQINPNFAMGYSRMGTIMSDLKQKDNELQYYNQAIEKDENCYYAFICRGQYYYDEQMIDLALMDFNQAIQINPNYSLAYYNRGEYYYTQNIRHFIQRYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W1K5</accession>
    <name>A0A8S1W1K5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0780052</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MT-A70-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W1K5"/>
    <dbReference type="OrthoDB" id="10262526at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036396">
        <property type="term" value="C:RNA N6-methyladenosine methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007757">
        <property type="entry name" value="MT-A70-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829:SF2">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE MT-A70-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05063">
        <property type="entry name" value="MT-A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51143">
        <property type="entry name" value="MT_A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8182155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="539" mass="62808" checksum="6050BD9BD89CE668" modified="2022-10-12" version="1">MNKDKNKKVKGDQNRSRSRDKEKNEVLRFPPINQEQFRAVVIEFMLSDQFDYPISGGELYTYINTIFLTKTSTPYLRNLQIEPILNQLSDAGAINLSKLAVGIYLQDFILSISKEKLGQMNIQYSKDKPKQMPTVEEWIKEEIRSITSGQTFLKIDHLLDEARNSKTNVSLLPKIAAQKKRLIENVGNISLKYEDNVVQLALERKRAALLKTENYDEEEAKRKFTERGLQICENFTRDKCRNVQCQKTHFRKILKANTQTKLGNCTYLDQCPEQEQCKYIHYILDGNDVDWQKRVQTALSNHKSMAPQWINCDLRIFDFRVLGKFDVIMADPPWDIHMNLPYGTLKDKEMKALRVDLLQNDGVIFLWVTGRAMELGRECLILWGYRRVEELVWIKVNQLHRIIRTGRTGHWLNHSKEHCLIGIKGNPQIIKGLDCDVIVSEVRETSRKPDEVYGMINRMCPNGKKVELFGRPHNCRPNWITLGNQLPGVYLKDEGIRQRFMEAYPSVDISENNMNDNLNQMTNQENLNVIYNNHIGKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W4R3</accession>
    <name>A0A8S1W4R3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0800153</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W4R3"/>
    <dbReference type="OrthoDB" id="290926at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37028:SF4">
        <property type="entry name" value="ALMS MOTIF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37028">
        <property type="entry name" value="UNNAMED PRODUCT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8183245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="428" mass="50657" checksum="BC74C86200F1EF3C" modified="2022-10-12" version="1">MTNQSRASAMLIFVDNSRTLIHKLHELFKHMNESEALIIYICNEINFPQLSDQIIMVSYLNEFSTYCRTVGLLGLQESFKQQQSTLEETLLMHLITLKREQLIELSTRIVRIWNINNRDQKQGSSQENQQRKMSVSDMYTKSSSQLNHLYEQHILNEENQIVKRDQQINHLMKECTFKPQISKKSQKLELNSPAHIRLNNYAMDSKIKLQIVQELNQKQELKDCTFKPSINIKSIGNNQSVENPFDRLYQNALTQRNKTPRNNEDSQFTYRPQLISSPIKLQQFEGISVEERLYNHHFEKMNNVAMKQEELQQIELEQCTFTPVINQYGNSRQNEKVYERLYNHSSVKSVSDVKDHSISHINKKSESLIKKPQSENNSYVTQFYIESNPFDRLYQEHQRQEKKKKSNQIQYYQSIPFKPILNKKLSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W7M2</accession>
    <name>A0A8S1W7M2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0840044</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8184863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10474951at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EGF-like" evidence="1">
        <location>
            <begin position="163"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="1">
        <location>
            <begin position="266"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="1">
        <location>
            <begin position="395"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="1">
        <location>
            <begin position="559"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="1">
        <location>
            <begin position="662"/>
            <end position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8184863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2062" mass="240034" checksum="94E8847CEC6E277F" modified="2022-10-12" version="1">MVYLQFLSAIIAIQMLVLNVLLLKQDQPVYYAQIILQQRMDNVNQKWFKAIMNNNYHFSLKISAQLLIIQHQLKDANYVQLRIVDIVLSTRRMIQTNVHQDEFLSVGCSLCEDNFIFDFTVGLCLHQKPNLQFCLRSFINLDGTEICTLSSVDDFRVASEIINCEKLIINCLQCVLISSLQIKCVICAQGYTSSVTTGQCYTTPYNNSKISIEGDGLQMNGWIQLIQSFLMSFLPNSYHYPTPYSYLITPLPIVCMDGYQISQYNTCESYCSSDCLDCEGTNDINFKFQCKKCSLDYYRLPLRSKYQNQCLKCSPLCAICQPRDISEINQLNKHFIITDSNLLYTYKCLYPAEHCQFSQLQNEQALYNNYITNLNRQQRFCQKNEIFYGLLVVFECYNCNLNACLECAVTETGLACILCLDNFTAKNGQCQLKVVQSNYEQQLSFFIKNKCLAPYYITSTKGCKLCPIKNCRYCFEYKKNDLNKCTLYDNFETFQEDEFLSVGCSLCEDNFIFDFTVGLCLHQKPNLQFCLRSFINLDGTEICTLSSVDDFRVASEIINCEKLIINCLQCVLISSLQIKCVICAQGYTSSVTTGQCYTTPYNNSKISIEGDGLQMNGWIQLIQSFLMSFLPNSYHYPTPYSYLITPLPIVCMDGYQISQYNTCESYCSSDCLDCEGTNDINFKFQCKKCSLDYYRLPLRSKYQNQCLKCSPLCAICQPRDISEINQLNKHFIITDSNLLYTYKCLYPAPDPNIIIRQPQLTPQYCFSQNCTEFFQYEFEINYKTVQEHLTTVLPQFFYENDINTDYLNQIGIRIMRIMFLYLLPLDEQQNIQDYEMIKIESKLKQKIFSLSQVRFNIRKADILNNIFNFSLIINGFDVIEIQNMIFSINKNFYFKFENDESINLNIEDTYFLGSQDNQQIISFLTFAKDFNNLRLINLNFNNLWINDSIIWEVSFSQLGGKIEISNIHFINCTFINSSIFHFFGNISEIQIKDITFFQCTFLRSSAFNFLVEYQTNFNLQILQLNVQECIFTQSNLINNYQTQLLYMKEISIIQSNFTISNIIISNSQFIMIESYFKFNLLSNSFLFKAQQITQENELEIIFLSNQFINNQFKNSSIADISSYIQSNSFSIYFNNTYLIKNINTNYSKLESFLFNIHSQSLRIFDLKIIDCFNLQLFNLYYINKIFFDDIQLINSIQINKIPLNIECMNSQQLNKMFLKIQRFQLLNLMNIFVQNYFFVDQTLIYITSETQNFQDFQEQLYFYNLTFTQNVLLKSNAGNIYTFLTISSKHKQNITIHDINFTENIFHQYTNDLSQSSSGLTVIDSYLSKVEILNFYCFQNILINTSNSFQFINSNVTLIDNFIVSNLNILSNYLLEKYYNIQFQYQIKEQFIKLASHQFLTLLNKVGVLKLTTSMFILNNGKFDNILSQDCALFEITTKDQGIISIQNIILSNIEADVRQDLGIHGCITILAQNSLLKYQLLNASFINVFNKFGSSIFTIFPSNKYNKIQLKNILFYNCLSFLNQIIKLEFDKLKKEQNFVSLHNITIIQSINAWIDFFSNIGPLSYQEVSKLSSDNAIIYLVNCHITIFKFQIEGSIISSVFKILDCYNVRFQQFNFYNVSILFPFDLIYIGQVISEYITVALSQINIQSISFSQNIKDIELKKTLLFIKNSNLYCNPIFSVEIQYQIFNLQNIIEQIIENNIPVDSLISFFSFTNYSKFELQTITISNNDCLNCEHGIINFNIRNFKQIKITNLLCYSNKITNKGCMILRSNSTQSNLAIIKNSQFINNFGSQGSGIISYNINLIISKCIFLNNYASSLGGAIYFESSKNQLKLIETTIMENSAQVAGGIYFYGDNNLNEKNFIKSILLLNKAYEYSNNLVEVPNHLGLSLNNKEMNFYQTKEIELITNYLLLNEYITIQQGKKLKTKYLLIPSNQKIGNYCLYNPLKLDCYEQINEISISLRNQMNELLTNVSDSICEISQTILSKDGLIIKEQQFLSKLSFDNQKKIIILKINQTCNVTYVQKNLLIQYPLYLFLYGLYYRFYYQQEALKEQINYIPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W8L5</accession>
    <name>A0A8S1W8L5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0830218</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000083">
        <property type="protein sequence ID" value="CAD8184735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W8L5"/>
    <dbReference type="OrthoDB" id="305557at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="25"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="151"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8184735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="480" mass="56994" checksum="71446DCE542B6241" modified="2022-10-12" version="1">MQGLIAQNGSDEETEFIFDPHTIQLKETRSKISKANEEIKQLNEQNKELIIQLNRQNELLELKHKQNQQLQAEANKFKEQIDDREHKLLQSMKEKQQLQNELIAMHEILDQTTQRNDQINSEVFALKLTLEQIQQTVTMCKEETTQGLQRELKLKGTIDQLNKNLKQQEEQLRKITNDANKKDIQFKFEMQNKESIIQELRNQLQQQQQQNNEIINELIEQKNQNIQNSVYGKDYKPELSKITKDNEELKNKFKGLTYQLNIQKEKEQSYIKEITTLRQMQSNLSTEIERLLNKINEQKLELLNQQQESQNVIQEKDKNIYQLMNQLSLLKNQDQKINLHKSISHNGDEKLLRDIQLKNDQIAMLQNENNQLVKLIESLKNQQIDNQGTEKIKIKQLESELKNLQSFIYDSPLVVLFNLLEDRLSKQQQKSFENKNKIKEFWATQLKQWTEGFEELKQAFSGLKIVFKYDLKYANQTSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WAE7</accession>
    <name>A0A8S1WAE7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0790130</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000079">
        <property type="protein sequence ID" value="CAD8182796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WAE7"/>
    <dbReference type="OrthoDB" id="5772781at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775:SF3">
        <property type="entry name" value="SORTING NEXIN-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="2">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="2">
        <location>
            <begin position="120"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8182796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="277" mass="33249" checksum="DEED2D899516C4EC" modified="2022-10-12" version="1">MLVKRYRVQINHLLQNDNKVYYQITVTKVNNENETKITINRYSELKDFHDKLHKNVTLLKLQMQLPQFPGRSLFSKTNQNEEKIIKRKIELETYFNELFSIDKILSLTPVQQYLPIENLQNQQMNISIRIENYVIYDEVVIYILRFKNNQANDEWIYKKRYSEIKSIHDALLDQGFKNKLPQFPTRKLFGQTNENPETIEKRKEDLENYLNALFCTQEIQESQIIQFLISDSKKYHEKNLKLEQLKKSSTLKAKGGDLNKRIDEFPQRKNQQINENQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WAJ3</accession>
    <name>A0A8S1WAJ3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Metalloenzyme domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0840067</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8184909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WAJ3"/>
    <dbReference type="OrthoDB" id="113620at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004619">
        <property type="term" value="F:phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16011">
        <property type="entry name" value="iPGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006124">
        <property type="entry name" value="Metalloenzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004456">
        <property type="entry name" value="Pglycerate_mutase_ApgM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00306">
        <property type="entry name" value="apgM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31209">
        <property type="entry name" value="COFACTOR-INDEPENDENT PHOSPHOGLYCERATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31209:SF0">
        <property type="entry name" value="METALLOENZYME DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01676">
        <property type="entry name" value="Metalloenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10143">
        <property type="entry name" value="PhosphMutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006392">
        <property type="entry name" value="IPGAM_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloenzyme" evidence="1">
        <location>
            <begin position="1"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8184909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="409" mass="45629" checksum="2FEA54CFF3D64EC3" modified="2022-10-12" version="1">MKILFVIIDGVADVGTPETQFKTPLQLAEIPTMNQIASTGLAGLMDPVEPGLSCGSDTAHMSIFGYDPFTFYRGRGAFETMGSGIDMQVGDIAFKCNFAYMENGIVKLRRVDREFPEWGLPLIDALNTQLKTPIQIQANHATEHRIGLKVVSNDYSGELTDEIIGTDPLKDNLPLRQAKPKVQSEKAIKTAKIINELSDSIHQMLSEHPINIQRQQQGLPQANIVLLRGCGQRIKVQDFQEKYGWKGAVIAPTAIIQGLGKTIGLEWHHVEGATGDYNSNFINKAKKAIELLENNYDFVFLHIKAVDDAGHDKNKDLKIKYIQKVDEMLKYFVNNIKLQDAVIAVTGDHTTPYAYGDHTFQSVPFIISKLSLLNEKKNDIQFNEISCLKGCLGRFCGKQVMQIIKQYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WBF8</accession>
    <name>A0A8S1WBF8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0860197</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000086">
        <property type="protein sequence ID" value="CAD8186177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WBF8"/>
    <dbReference type="OrthoDB" id="300702at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="622"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="350"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="622"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="660"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="685"/>
            <end position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8186177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="704" mass="84710" checksum="157635E73047E9F0" modified="2022-10-12" version="1">MQLLKLNQFQPLSKEDKPIPGNIVICLQLGTNYVTFKSFALKYSEDCQHYYINFLLNYYEAFYTKDNSYHLEFQMCYFELLPQFDRIKNIPIQRGYKLKNLKENFNIKQIIDQVIAITSYKEMYFLYIIHFSTRKCYILDPQTHPQSRFSDHEIVSKTILNYFQQTFSVECCLLKEIKQDTTALIRSALLLDFIFKYMDESNNVEKIENIKIRSSDAKQLLEKIKWFINKCYNENIYITKEQRQQPKLMDKLLSYDEWKQQMNSDSFEKVNKIKDEFKSRNESIIKFNQPTSPDSSISILSLMGQNQLNQSLSSSQSGISLNAQSFLKQQLYNQEIPRRDHKINEDVITKQEFENLLRDAKQQIRQEVLEELKREQDEKMQQYELKLQTKYNPLDEQIAQEYLTQVKFQRYKDYLDYLMEYYYNNDKQEYYKLIDEYNQRLKDTALNGMVEVQKQEILKLQAITKKKILEQNQQLEIALYNQKKELQDIIPIKNKLQERKDDYQRFQIQQNKHEYKFQFANVNLTIDPFEFKTDQQVDEIRKQKTILDEQDLSRTEIKEMQNAWKFETSKSNLSRSSVYNYDEELEKLQKMYQNKPEPYQSNRQSNRVPSLQSYPQIQSQAYSNQVFSNKDPTSQRNLKNNDPISKQQSKYDPNSKRNLDLSNPQSEYSRLQIEQSQKNLDRSIRQPSKFDSLSQIEQFQEQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WCB8</accession>
    <name>A0A8S1WCB8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sel1 domain protein repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0850206</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sel-1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WCB8"/>
    <dbReference type="OrthoDB" id="272077at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050767">
        <property type="entry name" value="Sel1_AlgK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11102">
        <property type="entry name" value="SEL-1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11102:SF147">
        <property type="entry name" value="SEL1L ADAPTOR SUBUNIT OF ERAD E3 UBIQUITIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="669"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="670"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8185659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="720" mass="83568" checksum="6FB340A2D15C0FCE" modified="2022-10-12" version="1">MNLGIDQIIPNLYSTIGNPTQSNILEDCVLEFQKYLEKVIPNYLQDSKAKKFVFKDQMTTNVITSLLNLTNQSDFLKPYEKQMTTTTLNKLYKIVQEISEYEKIRILINQTILNIERDTNRLLQDLQDLFQLLFSSKSCGKMTLLQQFDQYVRKLQQYTFFWENLILLENQQATIKIQKDQFAKETDILNKIEQWKISIIQERNQISSSKPSSLILDQKKIIQTQSLQSKNILKALQQYSGLALQKNQLQSIQLFEDLAKQGNPLAKAILGQLILEGQICEGNYEKAYEYFSESADQNCPIGIYFMSKLILEGKVQNKVFGEAQKSTSKFDITNNSRCDECQFAISMLKIASESGHVESMIYLGDLYYQGYKLDDFTLESDYSTSEIYFKEANKQDNVEAKYKLAKLYQKMYKISIYKNRIQLVYPLLLEAKNNDYLPAYYDLAKILIEGIKDELQPNQIMAELILEQGAMKGNIDCTRLILESKYNNLVNNQINISEFLQLLDSLDQIQNKVENLTFYYRGKIAQTENNVQLAIQLFNLGSNLGCSKCKQELSLSSKKDLIKKKEQEKQEKQEIFQANLGFVQLVKQSDWRLRFRKQSIFSNPFNASQIENDQSTVQARMTASIIKTIDINDAEDKRKRVISDYTGFSKQQVSDQSILLSNLKLKPNDQHQINSNNQIQEQKQRKSSQFTKKEKPKFLRHLSQHAIHSQSQNVLNIQNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WDF0</accession>
    <name>A0A8S1WDF0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0820047</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000082">
        <property type="protein sequence ID" value="CAD8183916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WDF0"/>
    <dbReference type="OrthoDB" id="6612291at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015149">
        <property type="term" value="F:hexose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045263">
        <property type="entry name" value="GLUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503:SF8">
        <property type="entry name" value="FACILITATED GLUCOSE TRANSPORTER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035862170" description="Major facilitator superfamily (MFS) profile domain-containing protein" evidence="7">
        <location>
            <begin position="24"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="317"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="343"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="402"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="7"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8183916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="461" mass="52099" checksum="6DD322B05834F965" modified="2022-10-12" version="1">MNKLLLIILNCCLGSLFMGFCLGQMNVISSDVYNVYKIDDTFTKGLMQSLLTIGGGVGSISASILMGLFSRRKCLQITDLFGMMAIMIAFMDESKYALLTSRFFVGLVLGLNGVLVPVYINEMSPKEKAGILGTMNQLFITLGILTTFLMSSFQDFSLPIPFYKMMLYLPIIPCIIRASALSTVFKYETPVYCAKHHLNQQLHKVVEMIYKDKAEKMYEQFQSSQKSTERRMTINQLLSSKYRSRLFIGISLASLQQLGGINGIMFYSSSIFDQVTGQASKKVFYLNLIVGFIGVFTALLATVIIEQFGRKPILKYGTLWCFISLLMLTFVMSNSIGNTSFGSYLIVICIFSYLFGFGFSLGPLLFIYLTEILPDLGVSVSGLMNWMSGGLVAQMFPIIASYDISYCFALFSIFNFIAYMIIQQKVIETKGLDKETVDKYFENQKFDYIPYQEAYQEDRFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WE41</accession>
    <name>A0A8S1WE41_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dynamin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0880053</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8186912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WE41"/>
    <dbReference type="OrthoDB" id="317793at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin N-terminal" evidence="1">
        <location>
            <begin position="430"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8186912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="930" mass="109808" checksum="3212C6919CDDC05E" modified="2022-10-12" version="1">MDQYQPNEAQESQLAKTIINIVKNQKNIISQIKDEFPQIPQKSTVFIFLGEFGLQFLNFYAHFLTKQVIQVNKYYKLVKVEDQGKIYYLLNFPMILLYDDQNDEIAQNLCISKIYAEMALKQMEKCSFNLILDNQQIKGDDDLAKLDNVIKDSIFYFGSYYFNEDNFFLLRHQVENRWDYLQFSKSNGFFSYFSQKQQIQTQNVGQNEILNSIIKSGNIINVTKVKSEDVINYGKMITYLQKQYDSLKEQLNLNIRQFQQLLLYLQQMPILFYNTDATNEIENYLKELYQDFEDKRNLLTEKASQIVNVYKYINKVNKQVNACMINKRQEKVFSPQMKQFKEDNKIEQLKIKPLLSDDLNVKLNSIIQFFIKYLNTQIKNDKICEVQKLKLNLKQWCAYCHESLENTLYELKYITQRIQKGPEKEIENGIYFIGITKAGKSTIINSLLNPHKIVREEFLDQKCYRIDMETKFKIGEGYNSETQKISGVYIGKKEELNQFFDQDESNVFSLEEVLDQNQNQNCKIPQKCFIFDCPGFDDNSNQLMRIAHRISLYNYLSKTKNIIVFFVVDISQQNIEQIKNTFDPISDLLENKNALEKDFKKWTILILTKAKDGSRDQYLKFWKEVYKEYLDQDYSFYKKLFNDDDRCIEFPKPSKDITKNIKNLVDSIPQKLQNQLQQNERFHPTFKLVIDDKVWRLYEQCIPKAKMKLEQLINFLIEDLDQYILESDDTINQKKEQMQKIQNILFRQITQDTQITQENCEQILKGISDWVKGNHHLKHNDFFLQLYIEDMKSILTISKYCQNNNIAPFEIKIDNLVNKIKTILKMIEQTIQYYKNWKYTGYATSFITGIATCGTSLALLEVGVVANLARQFLLTRGSLWVATVSSGSLSASTSGLSVVLNFIESWKNTHLKKIYKNELEKQQQQEQQNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WFI3</accession>
    <name>A0A8S1WFI3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0920217</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000092">
        <property type="protein sequence ID" value="CAD8189014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WFI3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8189014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="109" mass="13062" checksum="A46123BC4AD676B2" modified="2022-10-12" version="1">MESKLKSLWGEVQKRGEGRENHNCLIGKSIYYSIDKVQQQILRNTQPNQRIRLKIQMKRAEIVQEHFQAIILFFQFSYKQRCQNYSKIIILSENECLIIQKDGNLYQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WG04</accession>
    <name>A0A8S1WG04_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0890160</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000089">
        <property type="protein sequence ID" value="CAD8187605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WG04"/>
    <dbReference type="OrthoDB" id="8693905at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06612">
        <property type="entry name" value="STKc_MST1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000421">
        <property type="entry name" value="Serine/threonine-protein kinase PAK 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF2">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="12"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8187605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="525" mass="60994" checksum="140AD7C92F409E64" modified="2022-10-12" version="1">MNETQINPENYYRLECVIGEGSYGQVYKGIQLDNGKVVAIKIVPSSGEIESLKREIQILRDCRCDNIVKYFGSYHSNGQLWLIMEYCQGGSVIELIKSLQNPLPEEIIATILYQTLKGIDYMHSHKKIHRDIKCGNILIDHLGNIKLADFGVSTQLVHTMADTDTVIGSPFWMSPEILLKSRYSKKTDIWSLGITAIEMAEGEPPYAHIHPIRAMFAIKNNPPNSLTDQSKWSREFNQFVKRCLILDPKERPSTKDLLQDPFFQKYCRSREYIQQFILKYKKQIESYKLSKQQKQHEQQFQFSQEQVGVPLDTLIECEEEEDLGTMIINNVDAIQMNETGTMLEHQYEDADIYEKAINRVSEYGLSSLNQQRTNTIIEESPILKKKQIEDKINQLNQEMQNEIKQVKLKYGEQINSLQKALNQIVVQKHETHKSVHEQKGFALSTNYFKQLEQKNKFSAPSTPVQSQSKYRLDQENLNLENQLKQTKYQSTNLQKLQLQIQNRINDVPKLDNSQKLIKQLINRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WH95</accession>
    <name>A0A8S1WH95_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0920068</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000092">
        <property type="protein sequence ID" value="CAD8188743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WH95"/>
    <dbReference type="OrthoDB" id="312599at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000885">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="28"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8188743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="621" mass="71994" checksum="E5D3502DA0A39614" modified="2022-10-12" version="1">MLVQQQQRQMPATPIPGNARKVIENFSYALSDAIGKGFSSIVYKGRNDETNEVVAIKVIDKKGLKTPLHYQLLRSEVEALSQLNSPNIMRLYKVYYTENNTYLITEFCDSGDLGQLVTKTGIQKEQNMQKVFYGIIQGYKQMKQRGIVHRDLKPANILLKGSVPKIADFGFATTPQTVLTMPNVNVGSPLYMSPQAFKNRYSEKSDIWALGVSLYELLFGQVPWQAGSERELAQRMATVPVHFPGHISDECKDFIYRCLIVDESRRATVEELESHIWLSKQELQPIKGNGFSSMFRSNQQSNQQQLLQNNTVATPLGDTQNKQSIQFQTRESNYGKENAIIVKQSQQSIMQQPLKSIKRVQESRKESQISQQMNQEYSNQREEEYWSESKKNDYIILAQLNFCRYLYRVSALMDKHRFVQTPYLREKLMFLVNKNIMIKLNCLNEVMQGQNTLGLEKFQQYVQNPQYTKLCTAIHQYNQKYTLQFNTVWLSLQNPDQKSQLAMDKKFDAVFDENFTEYESFYIILNSILRNAIKEIEYSLESKIIVQDTQQLLPIDIEGGVILLDYLITYFQLATLIQECFENPYEFAVDSKIEQIADGKPVRLTYGHYIEIRNKIKQLDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WLS8</accession>
    <name>A0A8S1WLS8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EML-like second beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0970188</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000097">
        <property type="protein sequence ID" value="CAD8190928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WLS8"/>
    <dbReference type="OrthoDB" id="674604at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF8">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="219"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="261"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="303"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="345"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="356"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="387"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="429"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="471"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="512"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="554"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="595"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="636"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="678"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="720"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="762"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="804"/>
            <end position="845"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8190928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="922" mass="102400" checksum="E5B8BC6ECF0327FC" modified="2022-10-12" version="1">MQSSSQKKEALADIFDEIKDVDIQIFRAILEILRKEKIVDCIGYLSDNQNQQHLESQILQQVENITQADKEQKLSVVRNDMRQISDVLRKLKDHDFNNQDFSSQENEESKLSLIQSIKDFQQIIELLQFLVHLTSINETLIQCGSNSLHILVQMKVDLRNKNFENIKIQNSALVGANFVRCNLSGSMFDNVNISGINLNEALLFHCQWKDLRIQELNQLAGHTKSVFSVCFSPDGKTLASGSSDNSICLWEVKTGQYKARLYGHTSQINQVRFSSDGNTLASCSSDKSIRLWDVKTGQQKNKLDGHTTHVNSVCFSHDGNTLASISNDKSLRLWDGKTGQQKAKLEGHTSAIMSVCFSSDGNTLATCGWDQSIRLWDIQTRKQKAKLDSYSKYVQSVCFSPDGNTLASCSSDKSIRLWDVKTGQQKAKLDGHTSYVYSVCFSPDGNTLASCSADQSILLWDGNTGQYKDKLDGHTGYVYSVCFSPDGNILASGSDDKFIRLWDVKTGQQVKLDGHTSCINQVCFSPDGNTLASGSKDNLIRLWDVKTGQQKAKLDGQTTHVNSVCFSPDGNTLASGSDETIRLWVVKTGQQKAKLEGHTYPVYSVCFSPDGNILASGSYKEISLWDVNTGQQKAKLDGHTSYVYSVCFSPDGNTLASCSADQSILLWDSNTGQQKAKLDGHDDEVRSVCFSPDGNTLASGSRDKTNRFWDVKTGKQKAILYGNTSKIKSVCFSPDGNTLASGSADNFIHLWDVKTAQYKAKLDGHSNTVYSVSFSPDGNTLASCSADQSIRLWYVKTEQYKGQLDSHGDYVRSVSFSLDGNKLESVINDKSIRLWNDKSGEEIKSSDKNYKGIPAQFKAQLFQNNLLPKSSNITILRISEAPLFQAKDALILKGNFINYEGFDLRQLFKSKGSCFLEDLKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WRR3</accession>
    <name>A0A8S1WRR3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">COPI associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0980058</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000098">
        <property type="protein sequence ID" value="CAD8191055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WRR3"/>
    <dbReference type="OrthoDB" id="313388at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013714">
        <property type="entry name" value="Golgi_TVP15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28128:SF3">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_46, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28128">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08507">
        <property type="entry name" value="COPI_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8191055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="148" mass="16797" checksum="E79226B910C61399" modified="2022-10-12" version="1">METKTWILILRWVNIVAAVVIGAMGIYQLVTFQMFSHFHFYEIWSIFTPIYSLIFCGLLLAIEFKRDFIADQFQFMMTCFGRGIFYIFLGSVVSYQPSGESSSQQVAGWIWGLTLWLVGIFYVVIHFVGPKSATAGLKDAVSAEYGTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X0F1</accession>
    <name>A0A8S1X0F1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1060085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X0F1"/>
    <dbReference type="OrthoDB" id="303555at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8194220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="189" mass="23057" checksum="A79EBF79EC19A1FB" modified="2022-10-12" version="1">MTSYYFEMQEFEASYHEVNEAALIFYDDTDGSHQDTDKFMNWEENQFKRSKSSPSKRDYKKNICRNILRQAVKSMRRGKQVEFLQMELQQETSHFIDYYQKNLHNINGFRSLKNQLIIDQKDTPQLKLWKCVFQSYMVWFLNQRASLFILNGEANNFKEYLRFKNEVMLFYSQFPEKWYSNEPAWKTCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X5W5</accession>
    <name>A0A8S1X5W5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1120068</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000112">
        <property type="protein sequence ID" value="CAD8196320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X5W5"/>
    <dbReference type="OrthoDB" id="302927at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8196320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="98" mass="11467" checksum="C86F1C7A09805E99" modified="2022-10-12" version="1">MQCLYTSTDTSIDDVYYEDEPSISQIKEFRETFFINRLNSEKKESRKVSFNELEVVLLFEESDPPLMVSEDLILYQKQTGTNLKLSRYPTTSILKKGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X7Q1</accession>
    <name>A0A8S1X7Q1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Diacylglycerol kinase</fullName>
            <shortName evidence="5">DAG kinase</shortName>
            <ecNumber evidence="5">2.7.1.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1140169</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.107"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the eukaryotic diacylglycerol kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.107" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDO010000114">
        <property type="protein sequence ID" value="CAD8197205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X7Q1"/>
    <dbReference type="OrthoDB" id="242257at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000069">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037607">
        <property type="entry name" value="DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000756">
        <property type="entry name" value="Diacylglycerol_kin_accessory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255">
        <property type="entry name" value="DIACYLGLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255:SF121">
        <property type="entry name" value="DIACYLGLYCEROL KINASE (ATP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00609">
        <property type="entry name" value="DAGK_acc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00045">
        <property type="entry name" value="DAGKa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAGKc" evidence="6">
        <location>
            <begin position="87"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8197205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="418" mass="47604" checksum="48F88540C34A0811" modified="2022-10-12" version="1">MQYQQFSESEQQQSTLNLGLFYLFYNSGSGSVKANQFCILDCADITLNIEGKDVQISFFSIQNVTSRNQGYSKLKEDLRINNKQIPIIIAGGDGSMMWVIEQMISQNIDINQIVIIPLPCGTGNDFSNALGWDTDIPGNMLENNYKILKQYIRCWQRGHQCFFDIWDITIQTQQDGYFQEIKKNEKGYIKQSVKDLDGMNTNSLEKKMSNYFSIGVDARIGYGFDKNRTTNRHINKICYCLQGIQKMFLKNPRVNQVVQFVEHFNHKEQKTLFKTVNCQDKNALTVPGNPATLVCLNINSYAAGITDIWKNAKTPQEFLQKNKLYSEKTSFSDGLLEFISFDSIVGIGSERVLSGNATRLAQTNGPLYIKFQESNDNTTVNSYIQIDGQFFCVVNPLSVEIQPCRILPQGKIKILMKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XB44</accession>
    <name>A0A8S1XB44_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1180046</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000118">
        <property type="protein sequence ID" value="CAD8198274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XB44"/>
    <dbReference type="OrthoDB" id="296603at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="196"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="328"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="363"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="441"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="6">
        <location>
            <begin position="111"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8198274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="540" mass="62732" checksum="BFADE200EC3496B5" modified="2022-10-12" version="1">MLQLSMQIRNSKVETRKSYQQKKFQLKSSTIKKILFLLYFVGIFQLENKQIIVDLMNISLLMIQIWRHNTKIQISNIDRFDLTICFCALISRQTIPQLQSIILSLCFFSFKNNLSTILKSVKLLKKIKNLSITIIFIILLTSYLFTYLYHSYEQTYGLFGTFSNTCITMLEILTLDSWGSRGRILEQQVGYTGIPSIIICAYVFLMNYFYMNILIGLILETLQLEQNQQLVQVNFLDEHEMKEQKINKKLSDNTHICNLMLGQFYQQIVMVFSIVSVIFNIYNNFMIVDNMGKLFDALDDILYSIHFIFLLFLDKPRIQYERSKWERMLLHFIAGPLALFLSHDLACLCNLCKLATTPIVRGIFSGTISIFPILFPIITVLLAIILLMAVYTSRFYSSFIGFKGETYYTTLQQAFYTFVQLLTLDDWDANVLPVFESQQIIMPYILFPCFIIISNVILLNILIACSCKYFKEIDYSINCQSLNEAHPDNLLVKLNKVAELQIPIINCNTPNTIQESHISQREGLVQICYNNKIINALLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XDA4</accession>
    <name>A0A8S1XDA4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1200083</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000120">
        <property type="protein sequence ID" value="CAD8198945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XDA4"/>
    <dbReference type="OrthoDB" id="289604at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8198945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="272" mass="32364" checksum="EB246859B25DE8F3" modified="2022-10-12" version="1">MKHFNHIIYLTGSQQEIEFCAISDTYKYFHPETMEEFIPQYSDSKPNIQTSIYYVPDQTIAYNKNICIFQKFAQISYYQLSDNCHIKQGINFDMPNIKFASFLFVASSDPLIVYFYNQLVVNDQYLMQISNVQNQLGNSQLDYKLRQFWIQVIESHNFPVNFKRSQLIQVDFQQYNGVLKVSQINNNVIHPQVLKHAYDMNKRMMLSKYNNIRNLIKRKLKNFQMQESIFIALQELTKDKALVQKHDDSFQILIDYNRVGQRVYNYVESTCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XEE3</accession>
    <name>A0A8S1XEE3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T1210037</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000121">
        <property type="protein sequence ID" value="CAD8199171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XEE3"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08215">
        <property type="entry name" value="STKc_Nek"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000097">
        <property type="entry name" value="Probable serine/threonine-protein kinase nek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="40"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="302"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="381"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="311"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="331"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8199171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="644" mass="75698" checksum="F949AE576590D37E" modified="2022-10-12" version="1">MKQRPTTAKVPKTIDQREIQQLHDLDSRQHQLIGSNLSQFTILNELGKGSYGVVYKVKSSQDGNIYVLKKINLTHLKSKHQAEALKEAQLLRKLKHPNIITYYMSFIEQDNLCIIMEYAEGGDLQKLLKDYKEKRKFMQEETIWEMSKELSSALQHLHENNIIHRDIKTLNVFLTKDKRVKLGDLGVSKIFNSDTALQGTRVGTPLYLSPELVQHQPYDYKVDIWALGCVVFYMAALEPPFQGENLIALGYSIVNRAPKALPPQYSTRLSQFIWKLLEKIPALRPSIQEVNTIYFQQRIQPQRISQPQTSNPKSYAMQAQETEKMKKTRKPQQQDLISNGSDTIYMGDVIDMTQKQVENEQISRALQYQQLLKDQQEKLEKERVEKERIDRERQERMDRERQERLEKEKQERLERLERQEKLEKERIQKLEKERQQKEQQEQERLKQLEQQEQKHVQQQVLKIQSNHVELYIQDIDDKQFQEERKNSFSLEQAPKFKLEIKHSKPQNNYKQNVQIRPLSAQARGSSFANISCRAKLIPVQQNQSVKDVKKFTILDLNPNSNQPSMHQDQPIQQEPYIKCPNSNGGQNTVINNVNNILNNQPNKTKENIRIKSALILQYSNKRQQQDQQSNQIVKHYTLKDLVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XF51</accession>
    <name>A0A8S1XF51_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1220032</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000122">
        <property type="protein sequence ID" value="CAD8199559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XF51"/>
    <dbReference type="OrthoDB" id="296519at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8199559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="209" mass="25059" checksum="B0B70A34E6AB1140" modified="2022-10-12" version="1">MRKRLGALHADSLEQYIQQSQYSQELLYQTKVNEKQFDLKEFENTDKDTNTSFQEEQQDQKEYFNINILIVDNSNQIGEVFKYCNTLKQPKSNYHLEYCSKVEQSNSKQVHFHFWIINQESPKYQELINVYFTIADAYIYIYHKYLELEFQLFTEKVKKLNNNQSSIIFKVANSKRVSSMNNLQENQMIIEITLQSLDEVLERVKSQFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XHB8</accession>
    <name>A0A8S1XHB8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1250015</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000125">
        <property type="protein sequence ID" value="CAD8200556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XHB8"/>
    <dbReference type="OrthoDB" id="299883at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8200556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="234" mass="27157" checksum="4EC48D40A2F5D0A0" modified="2022-10-12" version="1">MDFKSRFKGKMTIMNKEGVIGMVNKPSIPIAKNNSYLSQKNELSPTIQNLGHNTEGIRETKSHIFTNIQTSPNKRNLTNYGQKFSNAGQQIIKQNHVKTEHHYYLDDPKKQLNQLQFKMHQDFNQLNMDEYEIVAIPKTMLGQLRQQLSVDKYGLNKSFGTDKKIPVELSQLSPQITTKCTSYNQTPLLKKQNTFDQMLRPPFNKQRSEKMEKKNQIIQQAGIGTLRYNSYSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XIJ2</accession>
    <name>A0A8S1XIJ2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1250079</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000125">
        <property type="protein sequence ID" value="CAD8200684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XIJ2"/>
    <dbReference type="OrthoDB" id="298116at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="604"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="714"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="774"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="896"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="732"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="774"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="908"/>
            <end position="923"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8200684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="957" mass="110496" checksum="AEEF6EF441BDA924" modified="2022-10-12" version="1">MSQQIQQILKQQQAQQFLAQQFSNKPDKIKLSNLIPNQINDNFFNKKTNEIMDKKPSSPRSNNQATKPSQKPSQQIAIHKSNMSLQNKSHIVNEQFQEKLNQFSKRRDYSPNSQLSNYKKNEEFLQRRPSSLVNDEYSATPQINRFIAKSPTPSEIQESVASLLQNHQTISSPKGSNSQKPKTNPLQLGPYLSIQQQQQKSNHKMKGHNSANSVSYNLKPNILKKCDYGMNHTPTKVTTTTLNTEHSKSKSVLDEQCIRMIYYDKEKENDMNLLNIGTPSTYLHSKISIEQQSVGESLNSKFHQAKILTQQSPYQNTSGSNDHLQESFPVRTCINHRTKKAKYFVDEHQKQIFYCSQCAITVASQGKIVQDLSQLIIKSQESQILSFQSSSVDSNRHSNFSDANFKERELSGFLTKLECSHSQRADILNQMQSQIEKINQWYESQIGQCHKSKQVMQQLMNDACNNSIQILQNQRQESLKQLSNLYLVLQQHQLDAQNIKQDIEKNWTEVLEDIRMDPFRKIMDYYQSEFVKMNDFQTQLQSSTISVKSLSKTDIQSTLALILQNFQLSESEQQLILHSNSIFNQTIRTQPTPPKKRIDTVDSQIHQSNQQQRVKTDYSGSKTQPQNNETPSNFYQYFNDSNIYQLSASKLQSQQQSQQNPNKNNLYISFENKDIFMNILESEKNSKQSLHEDKQSATSNPIMILESYRESKKNISEDDGQLSEHNSHKSTPHSPASGQTKQSVQRTATKLEEHVQIPKDAFQKQKSTFRTLFDNTEDQQNSDVIQNDSSVSPAKSEKFRSILNKISNNQSKTQQFYQQMIQSSQKFVLETPDIKKYELQGVQCLQYLQDTETCKKAILSNKQSQLMDSVQQMNLCDDQQDPSEFENDEDKEVQQMFSSKEMKHFSDQKQMTYPSESSQNNSHLEIRKDQYQKTLFCSPQFKDQDILNISESSGDSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XIR6</accession>
    <name>A0A8S1XIR6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1260124</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000126">
        <property type="protein sequence ID" value="CAD8201053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XIR6"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45969">
        <property type="entry name" value="RING ZINC FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="64"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="613"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="688"/>
            <end position="732"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8201053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="751" mass="90063" checksum="8BF22AB8FFEC3C48" modified="2022-10-12" version="1">MMHHDNEDNIWNLYTKQLGLFLSSKITLNTEQLFIQCISIYYSLRSCMVLSYTRDQKVVQRIKILVDAFTLVQILITFYFNVFNIMHWFLNQNSHKIKNGETQLSNYLICDLITNGDYKTLSIKRILYPEYSNQIKQEHNLMFYELTQIDSIYMMISLLKRIDKQNYIIEMLPIYPGNVNQFYSISYKFNSNDNASIIGFSQYYNKIRNSILLIVLKQNQIKLIEYSLFDKQFRDVYIYSLSENNVPSNWNIWKSGYIIITYEQFQVLYSIGEILYEIQEIYRFDSQPLQMLTNFDGIDSNYILQQNINGIKLFIIEENKYQFYQKSVRLIQQYDLPYSNIAFHIDQIQLMLIYDQFETINVKIDYLRMDQFQQCQFNIEELPKMNLQSIQYQQRYEIESQQQINYVQQKQDCQMPCDLIYESLNISLIPYKSECIIESFYNQFINGCNKFNSCYVCMKQIGCEWIEEECQIQQNQYSLSFNQIRESSKWFNNKIMNCGQEIEFNSTYYGNVSKGSIFTWFYDANIYQQFSLLFNLQLDKLTNKNFIQQSICLGNDTQQLCDQIMISDYKSDFIFKGYYFRITIVILEDIVVNDLTVTFQQQDPYMDRQDFKIQKLTCLLLGAIILFGIIIYIANKRMNYLISLTLQDSKQSLDNDSLSNMMENMIKEKILIKQYFSKQILNYDEDKCPFCIEKYEIKQEIIQIFCGHTFHLECFEDWIRINTKLVRCPICNQTIEYFLKNKDQFKKSIDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XKL0</accession>
    <name>A0A8S1XKL0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1280156</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000128">
        <property type="protein sequence ID" value="CAD8201716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XKL0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8201716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="342" mass="41207" checksum="1197FC0F9B4C52F8" modified="2022-10-12" version="1">MSKIRKNFLIKFLNDSIFQQNFTSTCFKEYEVDNSNLQLNEQPILLFQGIFISFSFFFYFELVKKSKQKPIFNRVDSDLSGDETQKKRKAKILHFYPRHFSVFKQSFKVSFIFSIKFIQEILSFFFHRKYHPNQKLSVFGTMNQNKFADTILIFGNIFFRLNQNLWNLKQMLQKFLHISIHFLSFYPPKSAPINSYDFNQVQEISYPPFQKSFQSSQLISRVLSILMIQQHHTMKGSDICVRIYSYDLLGYSCNSFVLKNKEKFLRLEYINKQDLDFIDIKVILIIYSFCLENTFRFKVLSNKSDKHFQFILNKKTKKIQRQGFFQFELGYKNLKCYQFIRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XKV4</accession>
    <name>A0A8S1XKV4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="18">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PPENT_87.1.T1280057</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 18">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>Na(+)(in) + ATP + H2O = Na(+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:14634"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Membrane</location>
            <topology evidence="18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="18"/>
    <dbReference type="EMBL" id="CAJJDO010000128">
        <property type="protein sequence ID" value="CAD8201524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XKV4"/>
    <dbReference type="OrthoDB" id="3352408at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008554">
        <property type="term" value="F:P-type sodium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02081">
        <property type="entry name" value="P-type_ATPase_Ca_PMCA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000039">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000061">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000045">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000191">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006408">
        <property type="entry name" value="P-type_ATPase_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01517">
        <property type="entry name" value="ATPase-IIB_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF369">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 18" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 18" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0915">Sodium</keyword>
    <keyword evidence="15" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="302"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="345"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="798"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="832"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="875"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="914"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="954"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="984"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="22">
        <location>
            <begin position="48"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="20">
        <location>
            <begin position="160"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase C-terminal" evidence="21">
        <location>
            <begin position="823"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1043"/>
            <end position="1066"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="CAD8201524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1066" mass="118696" checksum="106F20BEDE79E864" modified="2022-10-12" version="1">MSYKQQRDVEAQFQSQLTIDDLSDLFKLNSINDGSSIQKVKQFGDDTGLAKKLNTDLKKGFSDKNQIEKSKQVYGDNTPIEKEPTTLCELIMECLGDTMLQILLIAALVSTVIGMINEGVKTGWTEGATIFFAIFLIISITAGNNYLKERQFRQLRRKLDDGKCQVIRDGKVTEIQTKDIVVGDLLIFNLGDLFVVDGLMVQGSAVKIDESPMTGESDEIKKLPYFEMVQQQHNQLNVNQEAARGHVSPFLISGTKCLDGTGQMIVLAVGQNTVSGKLKQLLIQENPPTPLQQKLEGVASDIGKLGVLVSILTFIALMGHLGYDCYQGKFPFLSIKTLQIIVESFMIAITIIVVAVPEGLPLAVTIALAYSVGKMKDEQNLVKNLSSCEIMGGANNICSDKTGTLTQNIMQVVALWTENQTFKDQVHTNKNKIKKETIELMCESICYNSNAFPEKDPQTNKWIQIGNKTECALLECSDNFGFNFNQFRPSDKVLRQIPFNSKRKKMSTVIFNQKSQYIRVYTKGASEIVLSQCNKYIANNGIEQMLDPQLRKNIYDNIIQKFASESLRTIAIAYRDLDPQSHGSNIRGQISQLTKIAQNIPEDDLDKDLVLIGIAGIKDPIRPDVPNSIKQCHASGVKVRMVTGDNILTATAIAKECGILPTNREISEWEVVDGKKFREFVGGLKDEIVDGKTVKVVGNKENFARVSRDMKVMARASPEDKYILVTGLIAEGNVIAVTGDGTNDAPALKKADVGFAMGITGSDVAKDAADIILLDDNFSSIITAMKWGRNIYDCIRKFIQFQLTVNLVALFMSFLGAVVLKESPLNTIEMLWVNLIMDTFASLALATEPPNITVLERQPYKRDDKIVSPTMNRTIVGGSIYQICVLCAILFLLPQYMDLSMPAELIGQKFHKNVVQMSIFFQTFVVMQVFNSISCRQLDYKTINPFANACNNPLFWAVQTFTLVIQCVLIQYGGKFVKVSHLTLHQHLLCLGFGVGSIIFSVLVKMAIPERWCQFVELFRENEVQSGDMDTSLTSVLRRKSTNRLGNSRKSMDQQGSQIRMSAQRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XLF9</accession>
    <name>A0A8S1XLF9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Insulin-like growth factor binding protein, N-terminal</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1290127</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000129">
        <property type="protein sequence ID" value="CAD8201961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XLF9"/>
    <dbReference type="OrthoDB" id="304690at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8201961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="592" mass="67860" checksum="6E3ACE5C8E186E8B" modified="2022-10-12" version="1">MYDYLQIKESWGFKNFKLFLYLCPPGCLVCIQSDSEFDCKSWVLTHTAYTEIDYTLFITDGWTISYGDVNKQQCFMIPTICGYEVCGKDTVLSLNLINIPTHSQMKIKLKYLKIDSWEVSDYVQLKVDGELILDQQLSQLNEYLYGICGTTQKEVFVNIELTFPHSITSARIKILNTLDQAFSDESFGIRDIQIFIKQSLCGDQVVELQEECDDGNLFPFDGCFNCMFSCVDGCSECRDMICLSCYEGWIYLEYEQICEKEIIQEYSLITILELQTQFDCFPPIDNCQVCLLGKCILCQNDFFFNQFNDKCEQICFDQINSYYELCDEYQLQPFCNRCQIKCNDHCLNCNQGICQVCENDFILENNYCIQIGKVNLCQPQCQCQNICGNQIALYTLEECVCDLYCEECEDGICYQCNNYFKLFNNQCISTCGDLIVQEHEDCDDGNNIEFDGCFYCQYSCPIECIKCEQGICQDKCLPGFYLINNICSTICGDSIIAGNEQCEDNNTQEFDGCYLCQFSCPLNCEECINGICSNCNFGFKLIENKCTNVCGDGIMSNEEQCDDGNQESGDGCSQICQVEIDWICNQVPKSQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XSY0</accession>
    <name>A0A8S1XSY0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dynamin GTPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1360008</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000136">
        <property type="protein sequence ID" value="CAD8204083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XSY0"/>
    <dbReference type="OrthoDB" id="5061070at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1240:FF:000056">
        <property type="entry name" value="Dynamin-related protein,putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019762">
        <property type="entry name" value="Dynamin_GTPase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003130">
        <property type="entry name" value="GED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF21">
        <property type="entry name" value="DYNAMIN RELATED PROTEIN 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01031">
        <property type="entry name" value="Dynamin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02212">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00302">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00410">
        <property type="entry name" value="G_DYNAMIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="5">
        <location>
            <begin position="282"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="4">
        <location>
            <begin position="881"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="761"/>
            <end position="784"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8204083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="971" mass="112961" checksum="AFD183DDD88A8B46" modified="2022-10-12" version="1">MQKLLFTEEGLQIMRSRNSDPIKQNTDSKGISIRPKCLRIQSLSRQCNRVRVGTLKNTFNLSSPENLIQVLPFTRQKHKATSIPQVLSVDQLYGRQQEDLLQMAASQLHLPHGSGKDGMGRHHLQSIRLRTGDWYQWKNIPMLACVVDDFQKKLKLQQTSEEEEHMKVEKRQIQHEFEMKKVDQIQRKTKQNLNQVLEQKSLTYNIDLDEFWKQMKENSKYFQHHTDEMAKSRASKRERLEKFQKKFTDKKKQHIMIYENNQLWKNQYHSLMICTQAGLSSELNLPSIVVIGSQSVGKSSLLESIVGREFLPRGKGIVTRRPIEIQLHQVLDAEQGWFEFMDKKGEKIFESEDIRKLIESETDKVAGKNKGISPVPIKVKYFSKDILDLQLIDLPGITKNPVGDQPQDIEQKVLDIVMPFIKNQNSLILAVSKASDDLATSDGLKLARSVDPNGIRTIGVITQLDLMDEGSDALNDLLNQTYPLQLGYVGVIMRGQKDIQKNKTIIEQLKDEKTFFENHKLYKKYSEKMGVNFLVKTLNMNFIQHIKKALPIIRETIISLVQIKEFDLKQYGDYDNLETKENKNLLVLTLISKFSSSYKDMLEGRCLDITSKELIGGSRIIYVFNETFRRTIQRMNPFDVLSDDEIRTAIKNANGIRPTLFVPEGAFELLVRQQISRLRMPSIECSHIVFEELRRVVNQISIPEIDRFDTLANRISEVIENLLNKCLRQTEEIISNLIDIELGYINTSHPDFVSGMDMVHKDEQRQQTQVQQQKEKSPVQSENESNRFFNFWPFKNNKQTELYDSKLDNLNKKRKSNINNQKQQKLQQIEESQILQQQSVINKIVSVNQQYFVNDPKRQLPQVPNTIKITERPSKREQTEMDMIKDLLVSYFNLVKKNICDSVPKTIITFLVNQSRNFCERELIGMLYKQELVDELLQENQFIQKSRAETKQSLISLRTCLNLLNDLDSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XSZ7</accession>
    <name>A0A8S1XSZ7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1360106</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000136">
        <property type="protein sequence ID" value="CAD8204237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XSZ7"/>
    <dbReference type="OrthoDB" id="308429at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="27"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8204237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="193" mass="23185" checksum="46A24D42CB6BFB44" modified="2022-10-12" version="1">MFQIKSDNRLNRYESIEIERPKTSLDQNSLERENRELQIQLQKFKELYQQQKHYFQCQQEQFLDLKEKYEESVSKISELEDQLYQQSENQEQTQLCVSLLQDLQGLEYLVNKMKLLIPNKQALNLIGQIQKTYEQLRNKESVPCEQQIYHKLLTALDKKLSDIINNDKENSNMNMNIQESQPKLKTYLSQKMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XV13</accession>
    <name>A0A8S1XV13_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1390064</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000139">
        <property type="protein sequence ID" value="CAD8204955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XV13"/>
    <dbReference type="OrthoDB" id="313052at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00106">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000170">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF75">
        <property type="entry name" value="CENTROMERE-ASSOCIATED PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 3" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="5">
        <location>
            <begin position="3"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="371"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="475"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="97"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8204955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="710" mass="82857" checksum="351E2AB7611D8532" modified="2022-10-12" version="1">MENIQVVVRIRPSNGAERDNNDLEIWSVQNQDTITISNDRFNDLVRMRKFVPGQRVEFTFNQCFDSKHTTKFIYQQQIQRITLSSLQGINGTVFMYGQTGSGKTYTMMGYDQEEGILKQGLKDLFGEIAKQQDRQYFLRCSYVEIYTDQVYDLLATQERLSETLLINEDFNKEFVIKGAIEEVVTNINEIMDILQFGESNRHYASTVMNHCSSRSHTIFRLYVRCVPNYIGPNSVITESILNFVDLAGSEKINIHDSMLKKRGTSAGGSGGTQYKDRQNESKHINKSLFFLTQVISLRAQDKNDQHIPYRNSPLTKILRSSLGGNSRTAIILCVNPCYSQFEQTLSTLRFGTNAKKIENNVSKNIVGFDNDESLNRVIKDYEIKINELQKARVDDKQQQEMMLKIIEKLEEQRKIFRQQFTASDHIQAMINKELNYQWTHLHYHGVGVLWVPEKGNRELEVKQDVIDSFGTIKKYQELQEEFIILKSEKQSLENQVTDLKQKQQETIIQTKKRYANQKAKTRKYKQLSKDLQDEKNKLQKIALCYHNMIEMDEMMLLNNDTLDQMTENLQILMQNIYKVKLKKEIKLKGEYQETLKLVTSKPIKYPKYLFDTSAVNVLFWDATDNKKIEEQNKSEIESFQSYFQSQLNMSSPSYIELQGGSQQLKEPLKHICSHEKSSQSKIGKENYEPILKRNLSMEVSDELNFRSYFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XY70</accession>
    <name>A0A8S1XY70_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PiggyBac transposable element-derived protein 4 C-terminal zinc-finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1440074</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000144">
        <property type="protein sequence ID" value="CAD8206519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XY70"/>
    <dbReference type="OrthoDB" id="304889at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032718">
        <property type="entry name" value="PGBD4_Znf_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13842">
        <property type="entry name" value="zf-Tnp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PiggyBac transposable element-derived protein 4 C-terminal zinc-finger" evidence="1">
        <location>
            <begin position="303"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8206519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="366" mass="44062" checksum="BC118E83F142C2CD" modified="2022-10-12" version="1">MNSSVADIMDMAMLCLFRILETQSQIQLSEQSEHKYTSIIVFQRYISEIKAVFFYAYDSYSFIIIKQQHFDIDQLSSDLNKYYEAVEEFLISILKSNQEVCLHFKNYGPDFQEEKVDIMQPALRLIELPYLKSKIIIWINNQKIVQLKDGYLMIQYSQNQQVLLKFQQDIIKDQEIYEEFIRRQIKQVDDFHKEFIEQLELKEQYVKMYNIYSLIKDVTLIHDIPNSIYYLAKFLVNLPKFTCQETLVMITKLKLYQKNQMYLLNQKQPTKKNEGYKQLFITQLALFEDEKNLHFPMKGSHCQKCKVCLKQNNHKIHKSSFICESCHNYYNINVALCTDKCFKLFHLNPSYYLSRKRRRRQVKIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y132</accession>
    <name>A0A8S1Y132_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1480086</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000148">
        <property type="protein sequence ID" value="CAD8207721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y132"/>
    <dbReference type="OrthoDB" id="291551at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="342"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="375"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="436"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="501"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="501"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="522"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8207721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="531" mass="61864" checksum="D1A2942A95866BB3" modified="2022-10-12" version="1">MQKLNTYEATPWVAGYQCCIVIYTFISFGLILVSVFNQSILMDSAPQFYSDWDNKFIEDLKIQNTCDEGYINMLNFTWPGTELGCDCTQSNGTGKYKYTNYYYMECSEFMLAENCQPVEPIYEKQFQHWPFIDQTSVKSFKLCAKQSTSTAIESFQIGEANEGYKKCGNVFQIPSNELCPINDFTISTDQNGEAIGTTGLYLKIFRVQQDELPLTGFTIGFNSICYSISDIYQFQYLDKMINYHCYDNDERYVRLTNDMTYSDLLMVNEVQEEYNQLYKNSVIENRNVNLFYRYLTQFYYNSSELCQAEDLEFIYDLNSYINDNVMDVQLAAQVIVGIQAGMFGFLIPLISAISLVGCNFKYASLNRKSSLYKDLFGFWLGVKMIGEILCTIILASDMGYQQQMMDHFYDFINADCSDKFSLQEMSDFYNEYEIKVYNYVLLNFILCIITNVLDFYVTYLMCRDKHNDGIFLKKSTNSHLKNFTSTHPEELLNRTKVQPQSEIKLQNQQNRQFDDVEIPGDDMPRPHKPEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y650</accession>
    <name>A0A8S1Y650_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1520106</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000152">
        <property type="protein sequence ID" value="CAD8208931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y650"/>
    <dbReference type="OrthoDB" id="289886at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052788">
        <property type="entry name" value="RING-type_E3_ligase_ATL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45798:SF97">
        <property type="entry name" value="ALCOHOL-SENSITIVE RING FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45798">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL61-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="90"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8208931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="137" mass="16804" checksum="DC73B0CF6740775A" modified="2022-10-12" version="1">MNERRQQQFKDAYERVQEIKKRIHKTQIQQPRFPWQFMFLYNIYMNLNLKQLPQQIPQNLRIVKPRKPKNWFDDIPILVGVDEITQAEICTICLQEINDKDLIKILKCNHYFHNQCIKEWLIIKAECPTCRDKIQQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YB25</accession>
    <name>A0A8S1YB25_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Armadillo-type fold</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1610088</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000161">
        <property type="protein sequence ID" value="CAD8210969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YB25"/>
    <dbReference type="OrthoDB" id="292266at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001611">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="15"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8210969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="490" mass="58432" checksum="6CC2773EAA7C7D8A" modified="2022-10-12" version="1">MDEIKLVESDDELQYQPQTLKNQKQNQPKSVQEMREQFRLQIRKERIEEQLEKKRMWFIPKQRLQSSQLNFSINNLFNEFKIVYAQKEMNEISQQLHLIANIIDDRNRIHSLYLTQRNFLQFKDLLNDESYYIDTYTIYLQLTQDDEYFQLPIEDIQWSIERSLDLLIENKQIDDLANLILGYLGNVSSKLQQIQNYLLNKNTIDILCQLMKDSLLHNVCYLIQTICSNIPEQYKSQLIVILKHLKINKSQEILQTLFIINKQCDQYDKEINYFEIFQLLFNYLNEYLINENDFESFYSALLQMKYLSDFQQLYSIFQQFNILDIINKLINYKYQKIRTAVLYILQNLCKQGHNVIQQIFSQQELIKKLINILTTDKPINQSLVIRSIQHILKYGNQQDFTVLINLGLAIHLKSTIESAEPYIVLQGLECITSLMNYGLSLQTQLNVPNPILAILQQNQLIQAIEDLQYHPNKEIFVQSQKLITEFFRTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YDN0</accession>
    <name>A0A8S1YDN0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AraC effector-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1550043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000155">
        <property type="protein sequence ID" value="CAD8209564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YDN0"/>
    <dbReference type="OrthoDB" id="297115at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010499">
        <property type="entry name" value="AraC_E-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029441">
        <property type="entry name" value="Cass2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053182">
        <property type="entry name" value="YobU-like_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36444">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR PROTEIN YOBU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36444:SF2">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR PROTEIN YOBU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14526">
        <property type="entry name" value="Cass2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00871">
        <property type="entry name" value="AraC_E_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AraC effector-binding" evidence="1">
        <location>
            <begin position="5"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8209564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="171" mass="20081" checksum="EA5FB5B61ECB4365" modified="2022-10-12" version="1">MNPIPQKEIVQIPKFTVVGLRRRIYLAKEADQENNTFSLTYKEFFSKKLNDSIPNRAYPGRTYCLYYDFKDFQDRSQFNYYILVGEVVTEVGAIPEGMEIYQVEDSSYAKFQGGPGPIPEIVKQTWSSLHKIKPEEWGGKRTYKTEFEVYREGQEDLKNMKFELYIGLVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YE86</accession>
    <name>A0A8S1YE86_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1560046</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000156">
        <property type="protein sequence ID" value="CAD8209804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YE86"/>
    <dbReference type="OrthoDB" id="308560at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="110"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8209804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="343" mass="40185" checksum="0615E190AEDB98C6" modified="2022-10-12" version="1">MSDIVIQSDRKTFSYEILREYSIKQKEYCTALAIKKDNSLVLATADKAIKCFQFRQGQLKQFQYMNMFRKDITTLNLFENKSLFIIGSIDSSIRISSTNLISSSKYIQQLDGHSSWISNIIIHPIDQDVIISSSNDSSIRVWYNIQNWVCCQIIKEHKKEIKGLSINQQGNQLLSCSDDLQIFIMEPKNKKNYQFWQIIQKINIDQSGYRLCYINDNIFTFQQNSNNHLNIYSKDENDFYIKTGQLEVGGVGQSCDFLFPSIYYSNKQILINKNGCTINIIRYCNQINLNDENNCQFVLEQTIDYGDYSYGHIWGMCTQDQQYLITWDCDSSEIQIRKYQEKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YGX3</accession>
    <name>A0A8S1YGX3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1700018</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000170">
        <property type="protein sequence ID" value="CAD8212648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YGX3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8212648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="163" mass="19619" checksum="733B6C5B02E672BB" modified="2022-10-12" version="1">MKNKKKFPKSAYQRERLKSLQKKWSQKHSNIYAPQNEREQELRIPLIQVLFNMPLLNQSLQNLVKSQLLSLSCRLAIDQVLLRIQKILITHFKSRYQTRKYYIIKIIQMNLKDQLLSPGYIKLINLDYIHLFNHQTVSEDHPIKENNYGNKDDQKVYFIYQSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YH21</accession>
    <name>A0A8S1YH21_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1870014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000187">
        <property type="protein sequence ID" value="CAD8213816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YH21"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035816819" evidence="1">
        <location>
            <begin position="16"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8213816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="172" mass="20292" checksum="34EFCD0A22A03451" modified="2022-10-12" version="1">MFFFLFLLRIVLLNAENNFEIIYTAFSEDNFDKEGWHLQNSYSTTLFRKCGNETVFGGFSAFSNTLITYNSSLPPHYQIYASFLFWKIDSWNKEEKECVLIYFDDKLKKECFSVEQDYQLCGTFSEADFDKKVIFDIQMIHSSKNIFIIITSNLNQGAENESWGQKNLKLKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YHI2</accession>
    <name>A0A8S1YHI2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CP-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1620122</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000162">
        <property type="protein sequence ID" value="CAD8211262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YHI2"/>
    <dbReference type="OrthoDB" id="10266128at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04178">
        <property type="entry name" value="Nucleostemin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00882">
        <property type="entry name" value="Ras_like_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1580.10:FF:000002">
        <property type="entry name" value="Guanine nucleotide-binding protein-like 3 (nucleolar)-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:005957">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014813">
        <property type="entry name" value="Gnl3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050755">
        <property type="entry name" value="TRAFAC_YlqF/YawG_RiboMat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089">
        <property type="entry name" value="GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089:SF30">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN-LIKE 3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08701">
        <property type="entry name" value="GN3L_Grn1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="7">
        <location>
            <begin position="131"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="69"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8211262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="425" mass="48195" checksum="AFD26175EDFAC526" modified="2022-10-12" version="1">MAKFTNKPKGRTSKRLALNKKYKIEKKVKQHHKKLKKEARKMSALGQIKKTSSKEIGIPNMYPFKKNVIETLKRKKDQEEQEKKIKKLQQKEEIDLNLAEVKSNLYETKAQVVEEEQIQQENLNQLTKEHKKYISQVKKVVEAADIILIILDARDPLACRCKHMEREILGMPGEKKIILVLNKIDLVPPGNADAWLAHLRREFATVLFKANTQQQQSNLSSASIYKKTLSQRQDLADDLTSSSKAIGADKLLELIKNYSKNEGVKSSVTVGVIGYPNVGKSSVINSLKRSKACAVSSTPGFTKGLQEVVIDSQVKIIDCPGVVFDSENKESTLLRNIIKIEQIEDPRQPIEEILKKVSKNELLLLYKIQTFNNVNEFLCQVALARGKLQKGGIPDLECAARIVLQDWNQGKIKYFTVPPNQIEQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YHY9</accession>
    <name>A0A8S1YHY9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1060214</name>
        <name evidence="2" type="ORF">PPENT_87.1.T1730002</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000173">
        <property type="protein sequence ID" value="CAD8213028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YHY9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8194475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8213028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="49" mass="5658" checksum="E99B517185C57843" modified="2022-10-12" version="1">MINNKLIAKSPRQEPQIIPQIKAGSLRRILFSLFYLTVTMTKHQFIQGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YPR7</accession>
    <name>A0A8S1YPR7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1780008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000178">
        <property type="protein sequence ID" value="CAD8213394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="319054at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8213394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1721" mass="201870" checksum="0EA47A9F745CDA14" modified="2022-10-12" version="1">MKQTISFPFLIVICFEIQTYLCWSITILPTFYSYKQFEVKQSNSLDGESACFGVWSKYVPLSNIVQTGEYGILYSSCFHQFRIQKKVDSNVYFLYFECISFEEKMVKKYFQFLDSDNRVTTEQISIDLISYEGVRYFNGFLASPLEKRVTIYIQDQQMNQIFSKDVQIRFPFQENIFTQIIGGDFKVEQTSKFYLFENKILSYFPGQIYYLQNCFYDIPDQFFELIQSRQQKPCNCKNNQITDLPHVKIQNQDIYEFLSQNTNCQQFHLAGWIKNLEINKISNEFQFLLFKLMGNFQHPQLIQENLCAFQLLYKLSTQINQIILTTYSYTLPVVNIDFSTNPYLKKVEFDVTNDIQLWHYILVEKSDQFISITITFYQGQTQEKFSLTQEVVQFQMDFNDLIALILIICRQVVIQHAKNLMVQQKMIVYHVMNFQIEDMYQNLKFVYVNMELLIRIMSVLIIKHYNQIKLKSSNLINNVNMGFLKLMMIALNGINNYKQQCIFNPKSWIHTLLCQTSLLVHQDGSVTKYFEDENLQYLFAGDELIYCPNCDLNVKAPYDLIEASLIVKNLCSVSQSINENCYLCINDCKKCAILPTTELCLDRTPNASFANNTCYAPNYLNFYKKCVTCKLENCQYCFDYLASDPTKSTLENLESFSLNDEEIKLGCAQCREGYIFEFTTSTCINQKPTQQNCLRSYINLEEREICTLSNDFTLSFEKINCQDLILHCKQCIKTIQTTIKCLICEDGYIVSSITGVCSICPIFNSKQCQQDNLLEPGKWQFQGFVIQFLPPKTRINYDISRPNTIVIECLQGYRILENECQQYCDEFCSVCETVKQLPFSKFQCSQCKRNYYKELIRVQADRKCIECPSLCQVCLNRSTNEINKNSIYTFRCIQKVPFESKIHIDPILAIAQYCYQDNCNYKLEFIYVLLYQITQDEVECFNINDFKYIQYNYKYLNEIGLKYIPITINLELYCNFGRIEYILMNSFKENIISIRLSELIIKGSINPIIFNNKVIFQVQKYDQIVISNAIFLILFQIEMILRNRGQSINFYILNTKFYSNQSYPIIISINADSFQQFNMQNFTISDISIGNSIIFDIFCEGLNEDITINNIKLQDCKIINSTLFYFQNVNRNIFIKNFTIDSCIFIFKMNQNSISNVFLNELVISNCLFQNSKLIYSLDTTTLNINNLSIINNQILSSQFIVFNYNFYCNDIMIIDNYFLQLQLIAQIQSIQSIIEIQMKNLYIKNNHLTNFSVFITQQKQLFNNIDFLLLNFNFEDNTPSYKNENILITINCVTLLIKNFFLKNTTNFRFLFLLDIPYIQIDNQCMIIQFRNIKCLIKLIAQEFVFLSHNYCKFLGLIELLFRIYNVDLSLVSIYSNPLNTSNQNEYIYIKHSIMKGNILLKQNLGILFSLMEFYSKNQLYIAITKLTFEENIFHQYQTDPSQTQASLMYFDTKQSIIILKDILCIYNSLTNSTQSFLSISSDEVQLENITVLNHNWLNKEFWIKYYDILFQGEYNENEISYAIQQSFKIQNIGGIFSMFVTKFTINKGFFSYIQAESNSIFNIYLQGEGIGIIKNCSIIHAQNSLISNYENDGAITINGRKSLLKIILENITLEHVYNKLLQSFFLIYPSLKQNNIKFLNIIVKNCFSLVNSIINFQSEFQNADKNKIYIENLNIVQNEQALLIFLHSIGKISSLEFQKVIDDNAMMNFLGCQIQLIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YPU2</accession>
    <name>A0A8S1YPU2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1860020</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000186">
        <property type="protein sequence ID" value="CAD8213802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YPU2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="150" mass="18110" checksum="E9E9F5F96765CBF0" modified="2022-10-12" version="1">MSDLYHLILLLLLKIQKESIKTKEDLSHLMTLFLKLGQITAAFDKLWEEIISESEQKQSEIQKEYSIKQYYCISSQFNEQKLITQNKHQYVTSFLENINNNSPFRQDLDERMKIYYIFERKLLSQVQFRAIFNTKEAMKQRFSIQIQKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RXD7</accession>
    <name>A0A8S1RXD7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WD domain, G-beta repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0020574</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RXD7"/>
    <dbReference type="OrthoDB" id="303927at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001434">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="409"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="454"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="53"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="242"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8133721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="674" mass="80084" checksum="7EBF0FF0EF6040C4" modified="2022-10-12" version="1">MFQVYNIEDQDTGSIEQNLKQKWIPLQQKDTQIAIKALITLKSHIQKQMIAFIESSLKYIQDLDNQVQELQLKSKQIGQLVYELPYQVYNNPNKLNILLQEQLVDCERIKQLLKNQLQNLVNSDILINCFQTIQKINFNDTITSLNRIFHPHQKQKDGKWICDQHQQQIRYIELKGEISNKLACENCRQYNKYGQYITIQNMKNKWNEYIIENLNSFNLAQDKLISKSNIIYQQIQSLTTYFQKLIEEINQQLNNQTKLENDQLHQQFQMMQQDWSEFTNSQLIDVAYIYAQKNYIELIEQEFMAKDIQISKMMNQTIGFCNMLVVQFESFLINWNNPLINQISNKDKIEQTNQAVPLNYKLQEQYNIKEEKCLSFAFNSDSNVLVVGCNRFIKVFQFRQGQLTQTQILDNHKDYVRCLYFMNRSNQFISGCNDCKIMIWSCDSNNLWYCQQVLEGHTDYIRAGVIMNNREDLIISGSDDRSIRFWSKKDNQWYCKQILNGHSNEIRSISLNVSSTQLISCSTGTEIFLTQYIQNNKQWCIMQIIQTNKCGYSLKFIKDDLFTFQPYDQGYLLIYKRDQENRNFQIIQELKINSKDNRWDFFSQQLIEEKSILLHKNGNTMNIIKINQKNNEFSVVQSIQNSDVYLYGAMTRDGKYLVTWDSDSICIRVRKYQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RZD2</accession>
    <name>A0A8S1RZD2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0020088</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8132822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RZD2"/>
    <dbReference type="OrthoDB" id="286302at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8132822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="60" mass="6995" checksum="E836F3187FB71AB9" modified="2022-10-12" version="1">MGCSLRKSKPKQRDDIDQIEQLIQSDCLGMEQNFMLIKNPIVMRRNCSKEKQKLSDSKPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RZK9</accession>
    <name>A0A8S1RZK9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GYF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0020388</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6415790at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003169">
        <property type="entry name" value="GYF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02213">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50829">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GYF" evidence="1">
        <location>
            <begin position="114"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8133408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="239" mass="27963" checksum="CE51E6B9A4D7B98C" modified="2022-10-12" version="1">MNYKKETLELLGYENVECFIPKNLLNDFPQYFTTEQQPPLVTEISEMTENGLIFMSALSQNNTPQVSSQLLRSVQSKSSQGKIRIFFPQKWPVVKTNMSPFSFSRYSHFQSLIIRNWYYLDNSNNIQGPFSCVEMDNWYRKNLLNPDLLISYKSRDLTSFVKVKDLLKDSENPRCNKILKQMYKRASSAINNKQRISNDSPMSKASTELSXISNRKFENLTIIIITVIQKYSEIKFYIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S1B4</accession>
    <name>A0A8S1S1B4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0030065</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8133966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S1B4"/>
    <dbReference type="OrthoDB" id="309740at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="2">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="249"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="361"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8133966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="417" mass="50603" checksum="52A87513E4E66211" modified="2022-10-12" version="1">MQDQQSINIQINSYSYESTTIFYQISVKVGIKQWIVQKRYSQFEELNRILELTFKQLPQLPKKNFITFLVGKSIEQINERKYGLQHYLQTLASRIEILECEAFQSFLKLQQTEQFIKQELFKENIIEQQQQESPTQIKQEIESEQKLQPIPESLFISKRDQEQQTSFIENQVEQEKSNSIEQFENQQSSDIQQKESNENAELIVEMKAQLQEYYDQISEKDLIIQNFNQKELETQEYYNQQIYEKQYRINELNGQLNQYQETYSELQKQYQNMQNHMEELNIKFQESQQQSELLEQQLHQQQNFIQQQFTSIGYENLNQIQLQQIIIEQQKKLDEQIQVNKEKEFEFKQELDKHYLVEVQRDELFQENQLLKEQLEEELLNRNEQAETIEQLKLQLENLQMQLKEKQEILEIKNELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S231</accession>
    <name>A0A8S1S231_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0020567</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S231"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF32">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="130"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="543"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8133707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="562" mass="64662" checksum="03E353BC5823AAFE" modified="2022-10-12" version="1">MISELNSFFDCPDNSGKSNKIWQPIIDLEIYNNDIIVFQEDCRKISKKQKQLKLITIQLGNEHLYHIKDQKISQLNITVAVMTTYRHDLFGNIIRISRNGKFVDLLLDEYIVFKNMMAQRCLQTTFHDEFGVTKMIGKGSFAKVYFATKKSTGVNYAVKAFNKEFMQDQFKGRESLENEIKVMRRLNQENLVKLYETYETQNSIYFILDILKGGELLIKVKQQPLSAPSLQKLMYNLMKALCHLHSKKCMHRDLKPENLLLKEKDNDTDIVIADFGLACFLNEDILFKRCGTPGFVAPEILLYKEGDPFYDEKCDVFSAGVIFYVLLTGRQPFQGTDYKSILRANKNCEVNYEIKQIQSAPIQLVDLLKKMLYPDPKIRVSSEECLKHPYFKEIFKEQDLIDVQENLRDYDKDFIYGLGKQQGPPSQVGSMQLQQRQPALNGRIDTIGSFSNVSNNGSVTRLDQKPQLQQSKFSQFGNLNKQQPQSDQNSPVMKKNLQSDLRKTALKNSFQQQINQLSKDDDCVNDEAAHLEDAISKLNSKTPKMGGLKKANSYKIPKSSME</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S2K4</accession>
    <name>A0A8S1S2K4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0020486</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S2K4"/>
    <dbReference type="OrthoDB" id="79452at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45808">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 68F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45808:SF2">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 68F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13716">
        <property type="entry name" value="CRAL_TRIO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="1">
        <location>
            <begin position="217"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8133600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="431" mass="51413" checksum="C7B14208D377743A" modified="2022-10-12" version="1">MIIDNEKQQIRDLFANDQKYVQNEKKLQNDFVVQMQVAKYDSKNRDDPLLIHAGLTEQKQSVFIIQGKLQTDNNVKSITYYMISQLQNQKNQEFFIIYINTELKTYLNLSKFYSIFKEFPCVYFENLQKIFILHGNIFIKSWFWFQSSVQIKILKSKTIYLEKVVQLLNYQFFKKQQFEKMPAYCKDSSYQQLQQAFIGNQQQDICFQQCQNQLYDLELDQYEFTNKGIPIILDLCITKLLQDPQNIQQEGIFRLCSAQTQDKQFENYLIQKQYSEILDFENVIVIANFIKRVLDKLKYSVVPYQYYNQIYSMKQYSIEEAQVLIKQFPNLNRNLFTLIILMLQNVAQYSQINKMGPSNLAIVFGISLCRPQQYDQANVQEQYLKIKIVNQFIQFIIENASQIFPNQKISDFLLETGVEQNDYQQEEIQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S3R0</accession>
    <name>A0A8S1S3R0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0030011</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8133860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S3R0"/>
    <dbReference type="OrthoDB" id="300951at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="493"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="625"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="493"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="512"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="631"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="653"/>
            <end position="663"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8133860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="789" mass="92626" checksum="A33CE8E8A16DFA8E" modified="2022-10-12" version="1">MIQRKETSVSQNNEKDEKHSKLQKQPSSFFKSFSSSKKGMKDGEDFQFFMRTQDCSFYTILYMLVNFSKVEEDQKKKGFSLLANSKPLMNMETYNDLSVLHPELIYFFSVISVRDLHDFYIDFYASFDTNKNFTLEKKELTKMFTVVLEFLGHKKFKVFSDRTLIEADAFEGKLTQKLIEDISENFLLPEDQSKVDYLLIFPYILSFYLEYSYQEIKNKQKRLEIMKGIETIKGKEVEIKNITSLISILKEMGIDYLKEKNTYKLSYRDVENILLKFKNIQKWITFDVIQQILQSLSKIMQVDSKTQEETKKIKVHKIIDFLALKLCVHQNCLKIKQQKNIVNQSVFLEILVLWNCAQNSQWTSQKQFTLKFEKYLNSHYYEQQTFSRTELQKCLEGFFKKFFQLMPKQQIYQIIQCHKLFQDDNIVRNYKIQREDLIQSLFSMVCCEGFKKLRQYVKIKFDGIIKSTGNNAQYSNYGKIMDQESIQGGISSQRQLLEQSQQQNQNTSNNVKKKETKKEDSEHSEHSESSEEGNSNVKATQKFKTAVRAIQSGNAFIQEKNQGKDILKACLIMDSFLKRQTQRIKSHCKETKSLNSSFIKQDLTKVETQQNLMENEDQSLLFDPSAQINQKRKDQKQSEICGIKKEKEKKGWETSTKQNEKPQTTEIAEIIQNQEKVKVGQNPDLVFTESKQKHKDRNQISNELQMVLGKSNDDFGPNNFKKAIRLQSSGEVSQSARPLIRQNEQQDNLVIIDETALEQMIKTNQNKTKIISKTQNRKSTRDDCQCRLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S5U5</accession>
    <name>A0A8S1S5U5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0030248</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S5U5"/>
    <dbReference type="OrthoDB" id="305194at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="86"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="335"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="398"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="529"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8134324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="602" mass="72497" checksum="35C3B0BBDE02FFD0" modified="2022-10-12" version="1">MYRIGDQMSKTSQSRENSNSNLEECMHNIQSNLKMMYGKDEEEVHLGSYNVNAVSGFHSQFLSNKDDQYDSQRMHYTSSQSNDFMLEDKNKQIRQLNKELADLRKGQQMTQFEQYEHYQKIIDSLKKENVLLQQRVLNNEAVQQLNSERVIEQQEQIKEYEQKLDQQNQQISSLNLQLKDTKDQLKLYTTKSQISKQEQQSQSKHYTQLQQENDELQRRYIKQQLENENMKVLIEQLQNDIDKRLETQNDSQVVEIIEQVLQTISQFVREYDYELQKSEYQYKFSNELKEIIKGFITQRDLSIWQNIDRLSSLIISLFEQLTFDVKQIHSDNIKIKNMTLQIESLEQMTVQLEQTISQYEIISSQQQNYLRFNDDQNKQEKEELVNVLINRTLNDQQINCYDKMIKNLEEVNQAQQNEIKELKDKLSIQIEQNYQYQKQYQEEQHQQKIYESKFKKQHYDSLFLKEIMNKLVILIGNEMTTKTIEQIQELQQKTNDQYCDEIDIKSKLEMKEQDIKLQINENKLNMNDMIEKRREIEVLRNTLRNCQKQIEINKEKIDILVRDLDHNVMQEKSQQFAISYLPKTFKKENENLAQKLQKTMRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SAT8</accession>
    <name>A0A8S1SAT8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0040528</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SAT8"/>
    <dbReference type="OrthoDB" id="1919336at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051965">
        <property type="entry name" value="ChromReg_NeuronalGeneExpr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46040">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46040:SF3">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="5">
        <location>
            <begin position="54"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="3">
        <location>
            <begin position="54"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="26"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="128"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="128"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="142"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8135994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="167" mass="19926" checksum="1AECAD3BB2A9BAA9" modified="2022-10-12" version="1">MQDKKEQSKFENFLIRFKALIESYQTITPEPEEQPKQQVTKQKKQKRQKDPLAPKMPKSAFIFYFQDKREKFQQQYPNLQFQEITKLIANEWKDLPKEIQQSYHDKAFNDRSRYSQESQLYCTQTGKKFNGKSLTKTNKQSAKNEKIQQKVDQDEENDSFGADIGQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SBT1</accession>
    <name>A0A8S1SBT1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nas2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0050457</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8137010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SBT1"/>
    <dbReference type="OrthoDB" id="304649at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
        <property type="term" value="P:proteasome regulatory particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000435">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040815">
        <property type="entry name" value="Nas2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035269">
        <property type="entry name" value="PSMD9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12651">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12651:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18265">
        <property type="entry name" value="Nas2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nas2 N-terminal" evidence="3">
        <location>
            <begin position="9"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="106"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="112"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF18265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8137010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="257" mass="30910" checksum="9A8929745F583776" modified="2022-10-12" version="1">MLKQKLVLLQQQRKELENHIEQLNQQLQVYYDKGYHKSLIDEEGFPRQDLDFGELSTYKNLRREFNEKNNDYKDLMKLLEQTMISYHQDLQNDPNLNNEIEAYTQKWREQQQQHSKQQQQQQQQQQQQQQQQQSSSQQNWKNEYSKQNENDLIKPFAYLEDVIKDSPADKAGFKINDFLIRFGIIDHSNHNKLQNLYEYIKNQQNKQINVKIVRLLTPQVSVINIDFSKESYIIMDLTITPQVWNGKGLLGWKMNMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SEN6</accession>
    <name>A0A8S1SEN6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">33 kDa inner dynein arm light chain, axonemal</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0070036</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the inner dynein arm light chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SEN6"/>
    <dbReference type="OrthoDB" id="273640at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019347">
        <property type="entry name" value="Axonemal_dynein_light_chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13183:SF0">
        <property type="entry name" value="AXONEMAL DYNEIN LIGHT INTERMEDIATE POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13183">
        <property type="entry name" value="AXONEMAL INNER ARM DYNEIN LIGHT CHAIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10211">
        <property type="entry name" value="Ax_dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0243">Dynein</keyword>
    <keyword evidence="3" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8138280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="238" mass="27762" checksum="46BC33639DF74F47" modified="2022-10-12" version="1">MIDQQFIKSKNATLVSTSGKPIKDKKKGRDAAPQSITNAQTEDILNSILPPREYTMEKQQLWIQCVSSTPAKREDVILLQENLDKKLQQRQARETGICPIREELYAQCFDELIRQITINCAERGLLLVRVRDEIRQTIQAYQTLYESSIAFGMRKALQAEQRKTDYNNKIKQLETECQDLSKQVEKIESTIEDMQRQDQEQQEHEEAKHRDQVNFLKNANKVYKEELEKFLTGANVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJN7</accession>
    <name>A0A8S1SJN7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0080417</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8140030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJN7"/>
    <dbReference type="OrthoDB" id="310791at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="430"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="209"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="440"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="452"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8140030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="472" mass="58305" checksum="F50FBE6C82F46BF0" modified="2022-10-12" version="1">MAYYREEPLQKGDYHNRIPNHNNHKTIKPVYNDDHKNNENYLQNPKNQQSYMEIEVQDRNNKQQYQGNNNDDDPFPDNPYYNKAALDKVCNKSIITYKDQPNRFQDDLKNNELQRDNYNQINQQQQNEPITKYYNNDQRDRYQEVKNYYYNQNHQQKSILSSKLYSRDHLDQQQEQQKQTQNKQLVNQQQIYQYTNEDLQENPSFGQIQDYQQSQQRQQQQDYQQPQQRQQYQQQYQPIQQQFSYSHQSSQEFKNQNTQQYISNSTRQQTINKNEHPSQREVQQQRQCVTYQTQSPQRFHQDLSIQKQQQYQQRFQYLEELKSRIPKDDMFYSQTDKRMSQTEQYQRQEQNERITRINPIAAVSRIQKPNQINQDNQHYSKDQYQQYQQQKREDYLIQSNRNNSNQYLNLYKSNKHYDNYQNVQNQQINNYNQKTRTPNHYREDSYERERQQFIQQTSHIKTNSYTNNNKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SKW8</accession>
    <name>A0A8S1SKW8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">LSDAT prokaryote domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0090125</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SKW8"/>
    <dbReference type="OrthoDB" id="310870at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041482">
        <property type="entry name" value="LSDAT_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18171">
        <property type="entry name" value="LSDAT_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LSDAT prokaryote" evidence="4">
        <location>
            <begin position="251"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="161"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="180"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="190"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF18171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8140538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="452" mass="51254" checksum="6D3C8D3051CAB843" modified="2022-10-12" version="1">MDNQLIAFVGDPAVGKTSIIKYLKGIEFDEDYSPTEQIESIDVTIQNNETITIYDTPGNATERKQVIEILKNVQIVFVCFDVENIDSFDSTLQWLDDIKKQINNSMQVCLVACKIDSDNRQISQEQAFEFINDYPNVEYLEVSVKTGENMDMLIDKIPITTPQGDGDLQQSYRTQQSNKQSEKPAHSPERQSQTYKSQSSMLNKEEPLWKVYDFLFPTGRVAKLVKISDVNAKNLSIQDILFKLQLNQSYPVVNLIGAKETNKGKFYAGIARACFNTDAVIVDSGISTGIEKYVIRRGVKLIGVAPEQEVKYPNQNTGFIDPFEISNGHTHIFLLNNKEKMMVFGLEGVFKINLCKKLAEGRLAKNGQREREQEGFLNELQQAVMANHPIIVIKGSPLCDQYFQNYNNPHANYSNASFGQLVRKGHFYGLNSLDSEDIAQYVHFFLTVTPYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SLI3</accession>
    <name>A0A8S1SLI3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0100044</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SLI3"/>
    <dbReference type="OrthoDB" id="285608at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8141303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="156" mass="17293" checksum="1363834032D644B1" modified="2022-10-12" version="1">MNRIANGVIGLALKNQVYFSTAKKAKMELTIRTPYKTILDKFDGFSRIVAKTNEAALIIQNRTPAAVYILPPGPLKIKFTQDVKGVTGDFLHLGGYVFVNPDNTCEINLMDLVDRKEAKVDQFDKADVKDADTVAGRYAGKIRRAAQRTFIKKATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SP88</accession>
    <name>A0A8S1SP88_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Alpha-type protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0110082</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SP88"/>
    <dbReference type="OrthoDB" id="422053at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04515">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00198">
        <property type="entry name" value="vWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004166">
        <property type="entry name" value="a-kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056861">
        <property type="entry name" value="HMCN1-like_VWA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052969">
        <property type="entry name" value="Thr-specific_kinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47763">
        <property type="entry name" value="ALPHA-PROTEIN KINASE VWKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47763:SF5">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_25, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02816">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25106">
        <property type="entry name" value="VWA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51158">
        <property type="entry name" value="ALPHA_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Alpha-type protein kinase" evidence="7">
        <location>
            <begin position="494"/>
            <end position="739"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8142323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="903" mass="106752" checksum="2842452D681F70D7" modified="2022-10-12" version="1">MNINNVNDICNNTIFCLDDDCQSTHERKYVGICLEYLKYNVQDEEYIYKNKKCFNDNCPFYHFNLEKVHLLLQISPINGIWCFNLCPQQICQQSQCQFIHRKWAKGICLNNFTNICKNKNCQNRHLSWDKLRKEVYIQYNVTQINPENICENDKCNCIYHLDTFQNMCLNYFKGECPNKKCLKIHCDWEYLNQISFQKQQLLPCTTLIAQHSFINFLQISKQTEIQQVCDKQKLIKFQREIHQSNIIDVIFILDLTLSMKRWLQAIKMQIANIIYQFKEKINGYGVRIGFVGYRDKCDEQEQLVYRCLTENFDEIIKVISKQVAQGGGDQAEDIVTALEQGLNLNISRHQDSILCTFLITDAPCHGIQYHSDNISDDYNYTVEPGYLENVILKYKEAKRLSFFTCFKIRDSTDIMFQKMQDVFPDILITRKSQPNDFPELVKFAIESSITQSFNRSIYQKKPKSLYTGAKFTKPKIINYQYNQTQIKKSNFWKQFNEIVDQFQRQGVTALEINQEPEITQINIKNYQNQRNNVLDLNNTCVFKIFDALNNRYMVAKLSKEHVKKYQENQLNENDIKLAEEEAKTKYYVAAYANQLAYLFRQKTKNFDEIPPIFYVSPILYTLEVPFYGVHQLYAETWIDSAKFQWKKYSINGVFTSPQFYYYSAFSHFTYIETEGILVILDFQGSDNIFTDASIQTQDNQIPILEKDQKNNKQIGITTFLQKQHEKCSILCQKLNLNRINFSIANPQIDYHVWNLQDHQMISITCETCSQLRLYSLDQYQQMKEIKCDICRDLEKQPIEAICRCCSQMYHHDPNEDLLSLTVYGYCKFCKSNCILKQKRCLYCLKHCQLNLKQIGNLNQYICKNGYQYLQGLQCQLCKAQYNFNQILSEKDYNDGNYICCQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ST30</accession>
    <name>A0A8S1ST30_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0110094</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="311102at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035855866" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="16"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8142347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="319" mass="36964" checksum="056602A5DF21B369" modified="2022-10-12" version="1">MQILIILLIITHTYICKIISSCQIYSDNPEACIEKTTDYKCNFHYFESKCILENSISLGCIENLNQGACISQQTDLYGKEVQCVFNKKCQPVLKTELQNQGCRTTYNKYACFNVVNADCEWKNNQCQPLEEIKETAYSCEESYQKSVTFVTCSKLQNLLCISSGFTGEYGCITVQEQQLKTLQCTEKGLTKQACISIQTEGQECIFKDQMCRNIEESEINTCQMNLNRWACLSIKNPKISCQWIENSCYPYIYLNENQQNEILEVNISVCQSIKGLYRYDRKNTKCIQIIDFQXLLKIQTLYTIFVHKQENSFLQMNIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SUZ4</accession>
    <name>A0A8S1SUZ4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0120437</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SUZ4"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052591">
        <property type="entry name" value="CML21-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23064">
        <property type="entry name" value="TROPONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="181"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="588"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8143853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="816" mass="96238" checksum="15561E86A22DF31E" modified="2022-10-12" version="1">MIVRRDRIHHQGKTNRDFKMQDSLKYTSNNQNSSLDGDRIATLSIPKNKKFSEILDQKSKRIVQCKDPVEETKQSAKKFYQHHLIGYPRFSQLEEKELKKRVFCGDSNTLFFDDEHRKSQESPTHTKQEIKEKLAQQKREENTTGIQETQLVGLENKNTLEGKVDLEKVRDIRRAIRRRYANRKNFQKIFNLWDEDSNGAVSVKNLYNMIQRLGININIDEARVLLASADMDGSNDLGLDEFLDLIFNDKDALNVNLKALPALTEDEKDSLIKNQETIDFLRKDAMQARDRRHQNQVNLILKNRLQQLGQQLTIQDELQKGYISYGRFERVIKKLEIDPSILSEQDVKILYENFKNQDDTFDYKKFLNHLKSFQLKEEVYDDPYSNKNQEPVDKKQLLLKSMKYQEDQLITLFDITRVDAYSLEKMKRKTKNLMNKIQRYIPSHTKFEEFLKNKIQGNNVNVKEFSHVIIQFLESVNERYQKFDLESMLSVLQFGQYETQKSDEIVRILFNETDQEFYDRAQLRQKGPAPPEKVSKVDQPLQAIENKEQDIEKQIQFYNQDAGMTWYIPNQSKELSDVLMKVENQLFNKTERAYKLFKDFDKDKDGYISQQDLKQKLEEMNILNGQEIGILINYVDPTNKGYATFNEFHDKLRAGMTIFDNAGNQLISINSQPGKTFQSAAKAFLPELSRLTEEFKKPFRPQTNHTDVRPSTRFGATPAFKNTFENFVPPKTSPMFMTQGERFSKNREQFIQDEKAQNNQKYESKLNRIRQYHQSIDQRIQSAQEQRDQKDASNLKSKQMAQWTYEHKAHLKNDYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SVF2</accession>
    <name>A0A8S1SVF2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0130010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SVF2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8144080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="225" mass="26607" checksum="EB870C4E6AAA0CFD" modified="2022-10-12" version="1">MDQINNKFEELHSLKRFNALDCNSILILIRKKEVLITLGQILRLCENLLNKVIDLQQYKLDHYYLISNRIWLKLTQNNKLLTIAHEDIEYQIVFTGFQCPIFENQQNKQSASTSIIQIMIQIINVFETNFHHKRIFIKHKIGQDNYIEYIILIIIITIIVWSFQNKINIEKTMEYGISKLFYTILHHFNFHEGFFKEDVILGLISIIGEIGIQQFIMFGCISPQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SYQ2</accession>
    <name>A0A8S1SYQ2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">cGMP-dependent protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPENT_87.1.T0120321</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SYQ2"/>
    <dbReference type="OrthoDB" id="100546at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004692">
        <property type="term" value="F:cGMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05572">
        <property type="entry name" value="STKc_cGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000154">
        <property type="entry name" value="cGMP-dependent protein kinase 1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000123">
        <property type="entry name" value="cGMP-dependent protein kinase 14-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000089">
        <property type="entry name" value="cGMP-dependent protein kinase 5-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035014">
        <property type="entry name" value="STKc_cGK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF37">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT PRKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="15">
        <location>
            <begin position="109"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="15">
        <location>
            <begin position="227"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="15">
        <location>
            <begin position="348"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="481"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="33"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="510"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8143622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="807" mass="92562" checksum="82ACDD333F66C9B2" modified="2022-10-12" version="1">MGNICRQNPINDKNDLEVTSSQPPPIQQMPPSKPEEVKTQAVEKQEEPAQSIDQAKQENEPKRKEQKKMAKIAAVIDQEVIYENVQKQEKVKSPFDYQLLLNAFGNSFIFGQLQPEDKVKVVDKMFYCTVHDGELVFKQGDKASSYFLIERGQCQIIINNEVKKTLKSGEAFGELALLYNAPRSASVKAIGDCAFWAIDRNTVRKAIESISQKDYDQNKEFINKVQFFESLTDDQKAAIPSALINLNFKAGEIIVNEGDQADSFFIIKKGEIQISRGGKELRIMKAGDSLGEQALQQNSVRGATAKAIKEDVTVLALARDDLTRILGDKIQLIMYSNLQRWAFERHAVLNKLTKLQVERIVSNMQQTQKKSEEVIIEKGQPCREIIIVLQGSIKYGKEVLEKGQMFGDKFLDQGENVKLGEPVVMKDEGMIAIITFKQFFEIIGGSLEQIFAKNEKAHDRFIKKEEGQKQDVFKHFQLDQLIYVKKLGQGQFGNVYLVHNKIDKKIYALKCISKAQIIEQNLEKHLAQEKIALETVNFPLIMHFTRSFKDNTYIYFLEEYIRGMELFDVIRDIGLLNTYDSQFYIGSLILCMEYLHLNNIIYRDIKPENIMIDEKGFMKLIDLGTAKNLKSKNGRTYTIIGTPHYMAPEILTGKGYTYSVDLWSIGVCLYEFMCGNVPYAEDADDPYEIYEEIQKKQLTFPSVLKDRKAKKLIEQLLSKTPELRLGSSYASLKNNTFFERFDYDLLINRELKPPYLPPKNKLHSDKDIQKAIQVGKLITEEIKNDLATAQNVYKPEKARDPNWDKDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T0C8</accession>
    <name>A0A8S1T0C8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0150004</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8145803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T0C8"/>
    <dbReference type="OrthoDB" id="306401at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8145803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="267" mass="30990" checksum="2F40A7D28EBE4C8B" modified="2022-10-12" version="1">MSTKQLVYQGLDAVTVSLTKVPEDFLQQVQVGFQIILGPKEVFQNFLFLDSTNDYSKLIEEKQNAWFISPFTGMGYPYTDAVRLDKQYQFQGNSLYQYSLATWWSAFTPCSHSHGFLSQVLTTKFYSLNLYQFEVKQKSQLGSTTSISVHFLENNQILNTTGEFYVTLEKDLTDIRIDINVVCPLGKWIESNFEKCLDCPIQKLYKQNYKCSNTLKELVFKIQYSKQAQAYSELLINISENLCDMKQILYNQKLEEIIMFSINLTDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T1K9</accession>
    <name>A0A8S1T1K9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0130464</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T1K9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8144914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="668" mass="77715" checksum="17D55E0582867301" modified="2022-10-12" version="1">MINETQNKLIYLDFFQWRQGIQDLEIDKLNEIRMKLVKTIIKIAITQDYCLKYYYDGIVLRKQYYFKIESELIVDISQNPGILTNMEQIKYLQWQGEYGKNMRKQGKWMALWNGEALIEVAGYFENGLKEGLWKQIFKSYGDQGQVYESGEYFQNQKIGRWNYFYQKNKIGGGSYNQIGLKNGKWIELCDQFQDNCQVTYRGEYKNGKKLGKWDIFYQNKYIGGGSYDEFGNELKNGTWIEISDAFYSNSDAIYYGKYKNGKKIGGWFIEDSLYSIDCGSYDEQGQGNKIGSWIELFDGFYNHSSVIMSGQYQDCKRVGRWNFEMIGETVCGGTYDDNGDKIGQWIELSDGYRSSSLITYNGEYKSGKKINRWDINYASRKIQQIGGGSYDEFGEGIKIGKWIEISVNSSPGKEITYQGEYQNGKKVRRWDTLYQNVMIGGGSYHNEGNEIKIGKWVELGDYFMNSQVTYNGEYKDGQKIGKWDTLFNNTVIGGGIYDDYKGYKIGKWIDLDQDFRIDKQIMYQGQYQNNVKISKWDILYRNQSDVFTEIGGGSYCEKGNGIKVGRWIELSEKFKRHQEIIYIGDYHNSKKVGRWDILFGKFQIGGGSYDDNGDEIKIGKWIELSSKFTDREKIILIGEFLNGKKIGSWVEKTKTDRGITQKEIIYDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T391</accession>
    <name>A0A8S1T391_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0150132</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T391"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00106">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000083">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF15">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="10">
        <location>
            <begin position="12"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="415"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="720"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="649"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="728"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="742"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="115"/>
            <end position="122"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8146016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="774" mass="88971" checksum="FEAC548403BDC931" modified="2022-10-12" version="1">MKPRDKEGSNENLRVVIRVRPPMAREIKDGKFISTVQAAPDNQQLCIFDYHSIELVPDEDLEAFVQNPANYTIHQFTFDYVYDQESTQVEVYETTAALSVDSTLQGYNSTIMAYGQTGTGKTYTMHGFSFTPNSDQLGIIPRSLHSIFTHIQMKSNSSTTFMVRASYLQIYNESISDLLRPDHQQLNIREDKKRGVFVENLSEWAVRSPPEIYQLMRRGNAKRVTASTRMNDTSSRSHAVFIITVEQIEETPDGKRAKVGKLNLVDLAGSERVRVTGATGIRLEESKKINQSLSALGNVIAALTELKQPKSHIPYRDSKITRLLEDSLGGNCKTTFMAMISPAIDAFSESLSTLKFANRAKNIKNTPMVNQDQDQGALLRKYQLEIQKLKQELDERSKLPLDNMVSELERERQKALEDKQEVQSAYEQRSKDLDQERQLRKQLEEKISSLNSQMLVGGQKIEETPQFQNALEKQQKLIREQYQEKLQELEKERQNIEEDKAQTDKYKQLLLKQRDIMIALTNRLNERDETILQLQEELDAYEKLQKELEDIIQTKQSRVEQLEDIINKNNLEIPNNFIMNTNLNTINTKQQLLLSEMPSSKMLLVADPSSNSYIQTLPQQSTIHHQSDAMSQYELKNLIEINTKLQLDLKISKIEQERYKKEVTSLQNQIQKYENDNTIDQAKKSVDIILVLLTKPANANSLSMVAQELQKLQNILSEKEGSPKRTIQKNQQESPNLKNQEIISKLISKPSKSNNLLIPQQKKMKQKSDDELWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T767</accession>
    <name>A0A8S1T767_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0160297</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T767"/>
    <dbReference type="OrthoDB" id="301636at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="684"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="50"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="61"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="78"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8147276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="732" mass="86425" checksum="96012558D09D0941" modified="2022-10-12" version="1">MIKHHFKLKFPKQDVVQTKSQTKSTFYIARRIIQSTSDRAINILNTKGIDKINNDKKPTPTSSRAYTTSHYRSYSTTPTNLKGQQSQNLDEQAQPFNTERQQSISLLSSEFKINTQPKSNRVVSLYQISPKEVPKTNIINPIFDQEKHYYQSSFRSAKDTQLNKDDLIKINRYHNDLIDENPIIMKQESKKKQSIFINITYQEYERKSKQIFKKHLQGTQKQQIFLQKVLKNIKDSQSEKSESKSLVRKYSKIWKENFSQTQFQEQQKYSKSSQQFKLYFNQFIEKCLNKFRLYQQITEFLDLIEKIQQINLNLFESVHDLRKLYIQNHNIKVSHTEIIEDLKFRYKKEIISFGMIDILTEVKDDEFQSQYFLPDSQGDIAIKPIEYFQLEITRNNNLYQICPIISKLDNHTKNELLSVESLDMDKIIYQNNIDEFVPNIQNKNILLRKFNAQLIYKKYSDILIGNYQNNNDIIDLEHNNYLQQQQQQIPLNLDKKAMMMNLRKQTRDAKIQARKSVLGFQNENLRSQKISINPKYQSNLLVPEASELQLKRRKSKSLQNLSEIEIDQEDEIIKTNESDQGDIISKYYKDKPVKYKQQLRLQNLQIFDYAIKQEHSGIVVVQLMIEHNLLDELIDYLRQNPNFSLNSRNAIGKPLIMIAAQSGNKELVQYMINQNVLLNVKDLEGNTALHYAVAYGYYDIADLLLLNGANPYIKNRNDQNPWNQNQQLLQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TGN1</accession>
    <name>A0A8S1TGN1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0200219</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TGN1"/>
    <dbReference type="OrthoDB" id="302740at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8150356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="426" mass="50735" checksum="B63B86702A183EA9" modified="2022-10-12" version="1">MNNFEQDFYQFVKDQLAKKRRQKDSSPNTPQDETDEMEKNRLFNQNVSKVLQQERKKMKINKVTREQLFDRRFKDFANMDTFLDIKQFLKHLSNQKIAKAEKKKKLKTLKKIDSDSDNDDLFDYAEIIQKSKQIVEKSTVIRHSPDIKGYLIQEDQPNSPKNDIEELKTEDDKFYNGIDQLFVDIKTKSEQLALQKKYEQERKNCKGSIQIIKIPQKQLEENLQEKEKMDELISKQAKQFKEQTEKVLTLLEKTTKQLYNNKQKKGPQRYIKKKKKLTEPIQQLSEISEQRTTFHQFYPETSRSTNKLPQLSPNTSKIYSHRTFLSQAALPDSRKISQILEREEPQDKIKNQFKQFIQKIDRAKASFDKSYQNDKELLSIQQRIIKNMNKIGEIPLESLKILNKKEFEISNLKLSKFKKLRPMQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TGZ4</accession>
    <name>A0A8S1TGZ4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0210407</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TGZ4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8151540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="44" mass="5147" checksum="84C903592519BD0B" modified="2022-10-12" version="1">MIWWILRITNFQIISVPYTNGSSVQKFDIFNANLSIQMINGSWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TKL7</accession>
    <name>A0A8S1TKL7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0220063</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8151706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TKL7"/>
    <dbReference type="OrthoDB" id="306605at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8151706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="469" mass="56482" checksum="9D9136993E74BE1A" modified="2022-10-12" version="1">MDQSFYQPYQLKKNSIMNYLLLSDQLDYQYDCLELENDQWTSISILKGFPQYIKIRIQDYYPLKIFFNVGAFKCNIYFSFLTSQPSEKNHQKHVVIKQQKIIVIKRPEEHKNYLFFSLLSDYSTSIKLKAWFQSSQYDRCYFLCYDGKEQFRMRSGFQNNNNTDEESINTNKNNITSVNIKSAERSLINESVPPLKSFSDNRRINSVKHRNEQISLKNKILLVQQKKLQLLSQNVIQKYQRMLVQSYKSLNQSRMMKKYINQWIINLNVLKFIEMLYAHFKINKILRCMNNSPKYYTQRWKQMNEKTSSLNMRVLMQANLTLSIFSKQLNEKISKQISELILITLNDTLFIKKQAQLQQSFMNFYLKVKLIQEFYFTQRNKFYLQMEQMIQNIRQQSPQILISKQRMKRIMFEFYQQLCTKHKQEYQKFSELEVPNVTLLTSVFAIFSKPKLNIMKNQTLLLQIIEQTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TL73</accession>
    <name>A0A8S1TL73_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0220173</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8151916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TL73"/>
    <dbReference type="OrthoDB" id="312707at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="145"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="281"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="339"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8151916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="371" mass="43025" checksum="FB32209EDFD23AC7" modified="2022-10-12" version="1">MQQVSPEVQTIFDELLKPVLERIEVLKDRDQSNELQIKNLFDQMSELKTLIGNLKPIKVDEEKKIVKQDPKKENHQNNDVKKIDRPSTAVTTKNPVPAKVKEPKPITAPKPLSQSTIVEKTTAPVDIEKNQPKSPKEGNQNKTKQQQQQQQQQQQQQVPQPKPKSAVQTQQKQQQIKETKEIKETKETKEQSVKQTPQQKEEKKIEKQPAKSKTAQPVQPIQAEKKEEKKDTKKTDNKTSQKQNTKQPEQKSTDKKTDDKKIVKKGKQTEDTLNKNEQQTDEQFQQQQQNITKTQNNSEQKQQIEQPQQQVVEQKEFIETKTESPELQNIEPKESIVEQSQNQDQLTQNQDVQEVNEILPEKIENQQQEVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TQE8</accession>
    <name>A0A8S1TQE8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0250286</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8154268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TQE8"/>
    <dbReference type="OrthoDB" id="285377at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="145"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8154268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="487" mass="57922" checksum="35F9349EAC509A0A" modified="2022-10-12" version="1">MQNFITRPSRTNTNEFGHRHEDSFDYNEFKNLASSLITSQHQKEWNHLPRLTQILLNQNLTVMFDGVIIKEQCTLYPNVLKIDNHYIQLSQYQIKRKQLIHNNKKCYLLLLGNDIMLFFSSFDIQKHWYNQIKQFCIMKNLLVKYRLQEQLFQDFYSIIKKKNRQQFSAYIINKQQPYLEHTFGLLHESKNHSILKIKRIYEDTNNYVMITERFEGEPVFNFLLENIQFMEVQVATLIYQILLLLRYLNEHQAYHGNINALNILINRESQSLEIAVIGFIYYPFKQGDDISEYLKWVNLNKYCWSGFEENQIPGIHSDLYQLGVLLYIITFYTKINQDLKRKSDPYKLFLKYMKELVIKYELIDETIEVLTQMKTPDQQFVHVFSSSQLDLIKKLLSNCSLSDALTHQWFVNAKQKAKPHNQRQGLPTLKTIIELKEASDGEASKSQTKRFSIQSDNYDSMDECPTVSYLMRNILDYEKAPCKTHHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TUV3</accession>
    <name>A0A8S1TUV3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0270014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TUV3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="345"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8155297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="467" mass="56746" checksum="5A5E8B14618FB656" modified="2022-10-12" version="1">MKETLLENEFKEPKVMIIHLNFIFFQVNKQICILERNSAVYKSYFQVEYYQYTLPQKKRYNSTRLSYLFQQRLRNFKINFQADQGFIKEFREYKKNHTPAPGVYSDQLLITEPGQSVEFNKNPQMINSFISIFQRDISHFQFPRMNDNSPDLQRTISRNKAYQRMKKLKQKKPNGEQKMTERMSTFYISNNNMGNKDKIYLQRLDYYRIIDSSNEIKKLEQPDALDNNWSKILQIQIINIQRAIWFKEIKRRQNQKVKIVNSCKRMKYQLQFEYYFNRKNKFLNQIYYAQEYSPSRLPQLKSFARIKQDEFMKLKQRKIELELKLKQKELETKQNQKQDQNIDQENTIFTQSQPQDSKKWKGVLKKSQMKEAERVKKEKKKKTLLSKFEQLTISLRVDNVQIKKKLLKKKSEVITFQKQKDNIKNEKMQWQTKIEKSNKRWKSGRINIIIQSVPEAEAKIKMKYQMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TW89</accession>
    <name>A0A8S1TW89_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0290012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TW89"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8156478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="128" mass="15359" checksum="08641AFC2183536A" modified="2022-10-12" version="1">MNLQTKRQDIKQQKVYKWDSIERKLMKINIQIAIKNDNLIYSYLGRNLKKEYLQDYETQPVLYQNMEQIKYFNWQGEYGPNKKKIGKWKAILNGEAILVGGFSKFQKRYYTTQMDLSQVYGNSLQKIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U203</accession>
    <name>A0A8S1U203_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0310130</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U203"/>
    <dbReference type="OrthoDB" id="292979at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8158042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="278" mass="33489" checksum="50E08719B9A231CB" modified="2022-10-12" version="1">MVKFVQILSLKNMLSKLITCKETLLRQTSFLDSSLNKNQCLNQIHSQEMQVKKNNEILKLLESKCKSLKTESQEEYFKIMEQTIQILDQQLQLINKIMKKQNNRNQIKYSSLRSNILEKAANYFYEIWQFDTQNIDYLKKYLDIVKCFKGTLCDQYLEGLCKYAQYTQDYEELAYIYTQIEKNKFSQTQKRCAMTLFMLSQDELYYEHFLLNESNLSDQSFEIRFHLFATQYFQQQNCYHTTKFHKQQCEYMSSEYQRSIAQSNLELQLSFMKNNSNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U5T1</accession>
    <name>A0A8S1U5T1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0340109</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8160014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U5T1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8160014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="205" mass="25229" checksum="F8E92A839F4DA95A" modified="2022-10-12" version="1">MDLVNQAFKQLYIQDECYENIKDKVDQFPYDEVYSLMEFYVQNYLDRVQNNLYSYSYNDYDSAKFYMEQVNSYWMRIQNIALELGKYQNTIDQNFKIEYFKIIRQIYHQREICRIYKFNQSIEPYFCSSIFQYFKPKITTHQDLMTNWLTYSQYEQGVDEIKDFEKRLEFAMTNLPDYEKKIITKITSKLGKNIINLMKKKKKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U6S3</accession>
    <name>A0A8S1U6S3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0340264</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8160294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U6S3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8160294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="353" mass="42593" checksum="8F435E5F6F3BC369" modified="2022-10-12" version="1">MELRKKQEIDFRESLYQEIDKIQKVFVFQCKFRFILPIQYPRQQQQSIPLILQEVSQQQQKTSLKQNLTLTQQTQSHMLQNRAQKQIYQLSIDLSYTQIMFAVIKHRWLELIKKIILNSLFNYRIHIQVCKFKLDQCGYIIKNGDLKYLIRCIFFELFQKFSYSLCHSELQLLIGDSHMSFYNQQNDELYYKLSLLFVESQNTKKVLKCFKFSYDLEYNSRKEFQKIILLKDNNRSNSNNNSNTGLLDYFGLAVNAAKHIQKSFQQQQAKYQPQPQNFYNEKKFYLINDDPQNSFSRRAIKKEVAPPSPRKKINHPKPIFYKEYQHNKYKKQLKILLRSQQQANQSEHIRIIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U7S9</accession>
    <name>A0A8S1U7S9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0350172</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U7S9"/>
    <dbReference type="OrthoDB" id="295999at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398:SF0">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8160788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="672" mass="78067" checksum="475614FF9C74B6BE" modified="2022-10-12" version="1">MIRKFIKKVDLLVDPYEFNISQKEKSLTPLGFILNNFLGGFISIVLLVFASFYIVGVFQKSIQNQNTIYAGTQSDQNQEFALSHDNTIFSIELSTLDGKILNDNTNVNDYFSLTAQYIQQQRIEGKIGFTRNYTALNQSKCSKEKVKQFNFNSASMTEEQKENLICFDGNFTLQGQFYEPYFAYVKLTVSVCNNSTRKTCKTSKEIENFINKGVNVDMFIKGSKIKQSLNSSSPADLFSTNIFWRLTTGIGDNEDIYMQPLQMDLKKIQFFSDIFNIENSERLYNGSVVERQERRQEPISISSGIGLCTFYLRASSDSSFYFVIQQDLPSTILSAVSETTALFLAVMQIIKIFYRVYNQHKTFEILMNSVFKFDLKSVQKRKLSQVNQSQTPGKVQQSEKNSKKQLQQIFDYTIKYSFYQYILYIIRKYFKSCKKIEDEKELVISQIAKSKIEFDLDLTNILKKLYEIDCLKLFFFDDDQLMLFNSFCSPVFQDQMTDQKELFDILTASYKQTRSINQLQQQNQANIDNNNQNIIKYAATSEIPINIRLAKLTRFFKAQLGAENAEELVDNFQKVNENKNKNCQLNDQLIQFAKQNKSLFNLVQQNYSAILHQQNEEEQRYLEDQIILDVPNELEYGRKNSEIVGSKDQGQSLKDLDIQQSPDCFQNRLQQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U9E3</accession>
    <name>A0A8S1U9E3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0370005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8161755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U9E3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8161755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="91" mass="10674" checksum="121DD9367E39523C" modified="2022-10-12" version="1">MQNANLDICYYQVNVFNVQSLVSSYMKLIQVVSTGIHNLLLLDNHQLILWMHKILLQLQIFILGSQRDTKHLSYYEMKIQDNATYAIKLCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UAA2</accession>
    <name>A0A8S1UAA2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0360068</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="302167at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="273"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="963"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1158"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1262"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1474"/>
            <end position="1493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8161234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1540" mass="182150" checksum="D374603A1EA9A574" modified="2022-10-12" version="1">MLKLFEPYYKGLIFLLSQFLQFEFAQPQIFQLVLIILNQVQPIAFNFYPLYNSEKQFEPTLQSLLISSRPYLFIVSEQSKTIIVCFCFAFQQIVITFTIYLIIKQLNQKFHEIQVSNLILQYICNYYYLVSSTLYCWLLEICMMNITKLYGIIFGTIMIIEIVTITIISDIVYESGLILRVSTQLPLNNNNVKYFIKPLRIFQIFIFFFLTNQKLCLLLHNILNIAIQSLHIYQILELKIYTKEKCALTSLVIASIGIIIAIESLIMQSRINYNLWLLISLIMIYLLQFMYKRQCLSLQTVDVRKLKFLFSYIENLNGNNQKNKLQMTLIIFEHQQNCLKNECICKKDINIKQKILQLIEMKFELFLEQRTKKLEQLTLDLAQILFRRRQFVKAQQVIFRVFQSKDNYQSKYNQFNISITTLCLLDFLKYQIENDVLIQMMERMILTNPNNKLIADSLKQFSINENSDQYIQMKLKKTILDKSLFYYNFIEHSNKLQGDINYPIKIIKQLQQFRIELTERYQQFPTSSNQNVLKFYLLEVLNDYIEAFDLNKNKALNDEKYQLFHDSVYNKLFGQNPSYIVAEINKELDVIIVRQSKKAMIKFQSIYGQKRKDSLELSVNDIIPEYVLKQHRTLIEQFFDEGTNKYYQQQNLSFLKLQDKIMLPIEMMIGLNQSSSQAFSFLIFFYEQMDYRSYLVINQSFDIINCSDNISNQILGNNQYSTGFISTKSLGTIFNIIPEFDSMIQSNVKNFFNHNIRLLTSNAKQAGQRYLDYVCNIIIEKKFKNENMCYMITLDNIRANNNRNYIDSRLDTSIFQRQQTNDQNIIQKLERMKEEIDEDIDKAFPYQDIYDISLIKKVNPLNQSETQHVESKLYMTDLLVDQEQTNPMQQQFKPNQKLFSPKESMNEEFINKKNFTFQNEIRDFASGSSITMIKKSKFYKKFEIIYSLCKSLKSSSKLIKANYNFILFILLVQVYVIINLSLISNIRSFINDMKLLSVRYDVTGPLESFSVSRFTQINYREMLGRKVINSSYAAQLQIFPRNNLILSFDKMKKSMGDVLLRSEIDQISNITYLPIMLYVNTNNTGQEYNVSKRSVIIITLNYQYEFKLRLDGAGGSFDNPYYYFTYKDYQIVKSMFDNLNVVILDITLERSNQEKQKWMFGYFPFLVLNFMITLIIILQQIRHNNEKNAIMKYISSIDLNVLEQEKLRLSYLQQIVIQDMDIVKGFSIDIDSIDNQLDKSNIKQKSFFSKKSNTKLINNKPILGLTLSIGHFLMFAAFSIYLFLTFRLYFIKYEKTAVFYQQLSDLGVDIPAVYAQKEILFRVKFRYPFLNSTDIDQTYAVIFNALNAIKKYQQENLNLSNKDYLFDEYLIDFFTSINTGNLCEFQSNELKQQTQSFCHQVMQGNLVQGFSQTLIYILNSVGVQQSDTQNFTIQPASTLLELEGSTILTDIIEIMKDQFYNNLVDSAQSLILNQQLFCIFYLVFEIILVIFYFTKIDKYNYQQFLYLKQSVYLFPRHTLIFDDSFYRNFRSIVNEENLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UCB4</accession>
    <name>A0A8S1UCB4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">60S ribosomal protein L12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0380126</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UCB4"/>
    <dbReference type="OrthoDB" id="285098at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1550.10:FF:000011">
        <property type="entry name" value="LSU ribosomal protein L11P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_uL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_uL11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_uL11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020785">
        <property type="entry name" value="Ribosomal_uL11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_uL11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00359">
        <property type="entry name" value="RIBOSOMAL_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL11 N-terminal" evidence="6">
        <location>
            <begin position="13"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL11 C-terminal" evidence="5">
        <location>
            <begin position="74"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8162565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="164" mass="17547" checksum="1D504001EA1A1F7D" modified="2022-10-12" version="1">MPPKVDPNEVRLINIKVFGGEGGPASTLAPKLGPLGLNPKQVGDKIIAESGKWKGIRVMVNLRCQNRNADVTVIPTSSALLIKEIGGYERDRKKTKNVKHNGNLTLEQVIKVARAIEEKSLAKTFTGTVKQVLGTAQSLGATVDGQTVKAIIGKINSGELKVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UCK7</accession>
    <name>A0A8S1UCK7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Histidine kinase/HSP90-like ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0360202</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the heat shock protein 90 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UCK7"/>
    <dbReference type="OrthoDB" id="307928at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16927">
        <property type="entry name" value="HATPase_Hsp90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000005">
        <property type="entry name" value="Heat shock protein 90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.790:FF:000001">
        <property type="entry name" value="Heat shock protein 90 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.80:FF:000001">
        <property type="entry name" value="Heat shock protein 90 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11260:FF:000001">
        <property type="entry name" value="Heat shock protein 90 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00505">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019805">
        <property type="entry name" value="Heat_shock_protein_90_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001404">
        <property type="entry name" value="Hsp90_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020575">
        <property type="entry name" value="Hsp90_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003555">
        <property type="entry name" value="PRK05218.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11528">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00183">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002583">
        <property type="entry name" value="Hsp90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00298">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histidine kinase/HSP90-like ATPase" evidence="8">
        <location>
            <begin position="31"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="220"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="682"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="232"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="686"/>
            <end position="695"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8161469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="710" mass="81759" checksum="04D3A46C2367C9E1" modified="2022-10-12" version="1">MSTTQNKEEQFHFNADIQQLMGLIINTFYSNKEIFLRELISNASDALDKIRYKSITDPDSAGLNVEPNFKIKIIPDKNNNTLTIQDTGIGMTRDELINNLGTIAKSGTKAFMEALNSGADISMIGQFGVGFYSAYLVADKVVVLSKAVGQQQYRWESQAGGTFFIYDDSENPNQLTRGSSVILHLKQDNLEFLEEKRLKDLVKKHSEFIGFPIELQIEKTHEKEVSDDEEDNKEKKTEEGEVQEEKSKDEKKKKKKIKEVTTEFEQVNKNKPLWMKKPEEITKEEYANFYKSLTNDWEDHLSVKQFSVEGGLEFKAVLFIPKRAPFDLFETKKKKNNIKLYVRRVFIMDDCEELIPEYLGFIKGVVDSEDLPLNISREFLQHNKILKVIKKNITKKCIEMFQELSENAEDYKKFYEQFSKNIKLGIHEDSANRTKLAEFLRFHTSKSGEDQISLKDYISKMKEGQKDIYFITGESKASVGASPFVESLKKKDYEVIYMVDPIDEYVIQQLKEFEGKKLKNCSKEGLELDQTEDEKKKFEELKSQFEGLCKLIKDILGDKIEKVQLGQRLEQSPCVLVTGEYGWSANMERIMKAQALRDPSMSSYMMSKKTLEINASHPIMTELKKKADKDKSDKTVKDLIWLLYETALLTSGFSLDDPAHFASRIHKMIKLGLSIDDTTIEEEDEKLPSLEKKSETANTEATKSKMEEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UFA4</accession>
    <name>A0A8S1UFA4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0380259</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UFA4"/>
    <dbReference type="OrthoDB" id="299496at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF25">
        <property type="entry name" value="MYB PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="2">
        <location>
            <begin position="116"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="116"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="174"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="2">
        <location>
            <begin position="194"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8162827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="335" mass="40397" checksum="9F57AFF601DCC29B" modified="2022-10-12" version="1">MIPPTWNEFLEFISDIQDNELDKPLLCQFIKIPNKCMTSIQIKQYFSKDFQKAKIKRSCKWGDREKYFLIWCVSKIIAKLQIKFQEVYLYQVFEKLSNILDLPEEFLLTKWLSLLNYKLKLQPWKQEEDDLLIELRQQYSGPKDWTIITREFIKRSQTVRYPKQIRERFNNVINPNINKNEFNKDEILFIFREAQNKDKNWAGISKIMPGRTDNQIKNVYNSIIRKISNEMRMNYYSEVDEQVIQNLIIQQNNYDPKMISSLLEEFKRKKPCVKTESCSDYSQTSEPYFQGVPFQMACPYISYSPFQFQNIYFCFPQFYLLPHQSQIATQNFNET</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UHA2</accession>
    <name>A0A8S1UHA2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0400282</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8164220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UHA2"/>
    <dbReference type="OrthoDB" id="270720at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="27"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="52"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8164220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="365" mass="42025" checksum="23F0D9BCF38F71EF" modified="2022-10-12" version="1">MGNQCASQCQCQQQQKEQIATEIIIAKSDPNSLKSKRSGSKQSIKQTYAKIQQKEVKKDTETEEHQNKVQRQNKAAIQIQKNWRGYLVRKKKTLKNSVKRDTKKNYLGEQVESITLPKNSSSFELETRPAYKFKSGAVYEGQWRGTQREGMGTQTWADGAKYIGEWKLSHANGKGKFYHIDGDTFDGYWENDKANGYGVYTHANGSKYEGEWKNDLQHGHGVETWFDGSKYTGIYFEGKKQGKGKYEWPDGSFYEGDWFDNKITGFGTYFWADGRGYTGQWLNNCMHGKGIYTWKDGRKYEGEYKQDRKDGYGTYTWADGKKYEGQWYDGKQHGKGKYIFPDGIIKEGIWKDGKKVKALDNTSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UIX5</accession>
    <name>A0A8S1UIX5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0400003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8163702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UIX5"/>
    <dbReference type="OrthoDB" id="190265at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8163702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="168" mass="19692" checksum="B5D0DC2AEADA35F4" modified="2022-10-12" version="1">MGVDNLQYGLGTSKIAGCERFVFNIAYRIIPVYVDATLWQFYSNYFTINAQKVGSQQHVIEIQIMQFLLLVFHLMEFGRSESHGELFGVQLATLYFYKVILVAFVFCHNKFTFDKNLIIFYKRICFVNLIDSRSAQEKQMNIRKSQLNYLLVLLSEENKFLTLLQLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UMI7</accession>
    <name>A0A8S1UMI7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Leucine-rich repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0400008</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8163706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UMI7"/>
    <dbReference type="OrthoDB" id="277458at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050576">
        <property type="entry name" value="Cilia_flagella_integrity"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973:SF8">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT SDS22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8163706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="638" mass="75484" checksum="FA78A989DD79A66D" modified="2022-10-12" version="1">MHIQNHPCLQIRIKNHLKLMLPTQLVFLKKQHTSQLKQINHLFLVNCLKLQKKKTKNDILCYSQEFQDIPSVEIDERYRYLVKNGTIKKIENEGNIIFSVLQQIQGIWVCYRRPMERANNLEKLSLDYLDLTHMPLLEGEEKKLRILTYQHNRIQCIQNLVSLPNLLYLDLYDNQIKEIDKLKQIQKFKVLLFPKNQIRRIQNLDHLTKLEVLDLHSNRIANLEGLSKLKSLKIINVGNHLITKLEALEELNTLIELNIKMNQIENIDHLQVLPQLQKLFMSQNKINSFPCIFNLSELSLENNPIQTNKYDYFRYKCQTFETLKILDGKPIDQIKQEILFAEIPKSEPIKKKIVNLQQIQQQQKSKKPNQMGLEIHGVTSEFTTKEEIVPFEQIKRNQQIIKIHNHNYNNNQTFQIKKGQTKKYILQSKLQQPQRETVPSLQNSLTSTVAAQEDDILISIEKQWTAEYRRIQMLEKNNQFNKKSCLQHQLVEGGHAEVEDDVFLLIYCTASAMVLPTQNFSQIIQIIHFQYRNNFEGQLHQQSIKIQSIQMKFKKQQYQIIQQIIALSCFNFWFIDFHLQFKQMEKTSGMMIGKKLNHCSLILINLCKFQKNCKILKIYNNQKVSKRIDPIFNNFINV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UND8</accession>
    <name>A0A8S1UND8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Arp2/3 complex 34 kDa subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0420108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the Arp2/3 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARPC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UND8"/>
    <dbReference type="OrthoDB" id="312975at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1460.20:FF:000016">
        <property type="entry name" value="Arp2/3 complex 34 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007188">
        <property type="entry name" value="ARPC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12058:SF0">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2_3 COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12058">
        <property type="entry name" value="ARP2/3 COMPLEX 34 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04045">
        <property type="entry name" value="P34-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU364015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8165232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="326" mass="38110" checksum="85CDB972ECBAA43D" modified="2022-10-12" version="1">MMLIDPQNKLLQTQIMDLIMKKDPRIVVAKDYNYSCTKLQYKENGRLFLSFTCFNFNEIFSIAGNYMIDKYYKDYTKEDPDVGFHLTFSFNVQSAKEEPKIQKNATEAEKAEIQEIKLQIRAENQKLFEKVTKDFSQIRRNFYASAFEQAFDQINKGQTASKFKYQSRENEVVYAIPDQDALNIFYEISFSDNVDKTLANLIITEIIDAKKNVKMAPPISRSNYQSSILKSAFPEVQNIKVDPNSQLITMTLFKAQHFSKNIEQLSTFLQGFRQYLHYHIHASKTYLHSRIIKRISQFQRSLQLCQFEPEVEKKTEDIFTSSGVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UR48</accession>
    <name>A0A8S1UR48_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0460226</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8167095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UR48"/>
    <dbReference type="OrthoDB" id="299750at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8167095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="616" mass="72616" checksum="15BACD1DAA7E2597" modified="2022-10-12" version="1">MKTKIVSLFLKREHLILLSKMLQLSENVIESDSQILKLDIQNQQSENKILKTERWPQLDLDYFQNNFLQIANLSFSYFKLHFPANSAIHIKDHIKFIQILKQINYGGVTIEDVFKQQRSRLQIEVTKITKSIKTVDEPIMPKIMNYKIDLEQIQTIQDKYFYPKNSDFLNPYLILNLKMPLPKSTARPLNKELYQSITSLEDVPSFQFMNSTLIQPILNYDMLKNSFEYRMNKLMQRNQQIQYTIKRYQLSIKNYDNINDDIQDSSLQEIKKIFCSSIKQIYDKKNKDIVLEQNKKINIDEQISKQSSPHQTEVFKENDSLLYKPIQEDEQQYQDVVTCTNYNERSQDQLPDFDPESPKLHSLRLTPYGFMETTYQQTLSQKMLPLDHQHQLTQSPLRAIPKYKGSYKSLDLQQSLILSPSTLKYPKKIFAYQKAQKLNQQNSQNDPNTKVILDLPIQTSENLEQSIETQTLDTQKAILTNRNTQKTCLDKIPLKPIAIQQIRLKKLKDKLIDKKETTLPYVRESLPRVQEVLMFQKKNNMKNQLQYQLILLTRSEQILTSLMSNGQIRGTELYQQLLQPIKLTTSQKFLSPKSNTLISRTKFKKIQQETKMFNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1US39</accession>
    <name>A0A8S1US39_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein kinase-like domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0440230</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1US39"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000737">
        <property type="entry name" value="Protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="10"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="292"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="328"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="760"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="372"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="801"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="884"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="415"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="373"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="394"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="886"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="947"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="977"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8166602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1053" mass="126005" checksum="ACB3A29A069B5E63" modified="2022-10-12" version="1">MSRNIGDFTFNSNEYIGQGTLGKLHQGINKKNGQEVVLRLLQKSLLKDDNIMSAALAEEMALMQKLKHPKINPIIDVIESQTFFYVVCPYRQSNLQTILQKQIKLSEAETIKLINDLIDGFSELIKQGIIHRDLRPSNILYENGSYIISDPGFIQTIESFKRDNKTKPQQFYYSSPQVLENKANYSNKCDVWSLGVIIYECLTGVLPFQTSSVEEYLKVCRSKKIEFQQGSMTGGMKSFIEGCLVYQEEKRMDWTKLLRDPIISAKLQNKNEEIGKDNKKYLMTQIRQKIIKKNLDLQEVYERIDENHNGLLDFDEFTAFISMIDKKVTREDCYYIFQQIDKDGSNSISFDEFKVWISSNNNRMAFFKINKPDNVQNNKLNNNPIQQPKNSKLNQPQEQYQQNQVNQQIPYQQPLNYQQQQQQQQQQQYQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQLISQQQLLNQYPINSPKQKQLYTQVQPQMFPQSQISSSGSFPAGAGFGRSDKLERAILIIRNSIKLKQIDILSLFQQSDYDKDNFLNFLEFSQLIWSLNNQLTVEEISDLFKVFNLNSDEYISFVEFRLVINLANEKKQIPSQQIPMNQPFQQNNNNYNTSIQQPNIQFRGNIQQPNQNNYLFQNDQFNPPQNYQNHQYVPNQGINNNKIQKKQNDALLLEMLNQKQAKKDQKKKEREREEAKKRQQNQFLPKEDNHQLQQVQILRTSKLKQQDKQKSILETPKYPKENISSQNKIPSSRPSPQNDKRKAEQPLITYSQPKNQEENQNQQKSSTQNVNQQRYLTDQYYNTIGSKLDQELAESTNIIQTSSIPNFNNKPYRKVLDEKIDKFYYSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1USB7</accession>
    <name>A0A8S1USB7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0460212</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8167067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1USB7"/>
    <dbReference type="OrthoDB" id="302318at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="104"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8167067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="208" mass="23924" checksum="88501C5845619DE8" modified="2022-10-12" version="1">MLPLTQNIYHRQSTSLLQKHFETRKNSPVTIIRAPALPNKQPISGSTLCIRTPYSDIKLRMFTQQAEESKFNSTMKQDDSQNQQVSSLVDIKTDKLSCTKTTVAESIEEEKQQVKKNVVKQSNLQMQKQLLDRDLEIIQLQRTIKILKDQNKKLITENSVLTKENIQMKTKIDSLEKRIEGSPTHARISEYISALTEVDKIKIESLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UYN7</accession>
    <name>A0A8S1UYN7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0510261</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000051">
        <property type="protein sequence ID" value="CAD8169811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UYN7"/>
    <dbReference type="OrthoDB" id="295512at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035942025" evidence="1">
        <location>
            <begin position="24"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8169811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="266" mass="29992" checksum="7EE16543CBE89BAE" modified="2022-10-12" version="1">MEKSYFKIQTIILIIGLFSYVKGSQLVTFEDMCTCEQLVYENECLANKICNFSNQECKTIPCDQLSAGNCHNNHKCALVNNQCVVFKSCSSHNAKTEEACDNLNHSCYFDDNDQTCREIQNINLGSCSEDLFSLCLIANEGLCIRKGGVCQEMKICEDSKSDDFQCFLAFPACEPKIDKCQSHLKCSESEWLDCRIAKEKINGDKYQLCMKGSNGCVDFDPSVQTKDTCWNSSSLFYHWDGKECSRCSWDSIIISVLSIALLILSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UZR2</accession>
    <name>A0A8S1UZR2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-dependent RNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0510211</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.48"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RdRP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.48" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000051">
        <property type="protein sequence ID" value="CAD8169713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UZR2"/>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057596">
        <property type="entry name" value="RDRP_core"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05183">
        <property type="entry name" value="RdRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0696">RNA-directed RNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RDRP core" evidence="2">
        <location>
            <begin position="4"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8169713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="407" mass="49157" checksum="F6C1E9909D84F342" modified="2022-10-12" version="1">MKSNNLNEQNCYFIKKVQEKLRKLEIQQIQKKYNILVEKAERLFGIVDQQGILKEGEVICVRKINDKLSYLEGQLIIIEDCIKSKQMILKVIGLSSKEVQRRFGDKKEYDEYINCIIFSNKQYLSTQFCTNTYFVSWDKRLIPKIQRINNKNKILQDLDFPKLEQEDIIDYLSDCLNNQITEYVECNHIGQYQYLDSNENIFENLDQIMMTKMQQHQGNDQNKVNIDDRFIYRFYWMKDENNYEKYVEQCLGQNHIVQALKCLNYIVDTFNGWMKLYNIKDEYEIYSGYFENQNDDENLDSFQELMILYLGQLKIELDNILSQIKIERQIQIWILHFLIIYLPNEMNIRNQQLERIVKCIKNQVQKFDECKFLCSRLETKSQQWFKGCSWFFFGKEILDFEQSSKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V145</accession>
    <name>A0A8S1V145_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0520226</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000052">
        <property type="protein sequence ID" value="CAD8170243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V145"/>
    <dbReference type="OrthoDB" id="1305at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="381"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="530"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="459"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="382"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="546"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8170243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="763" mass="87458" checksum="23223C1160636C70" modified="2022-10-12" version="1">MINQNTESSKWDWVPATQTLEITINSNKVQIETTPSIVLTHPEGDAYPEPYVTLVIDTEKQLKYSEELGWNWHKSWNLPNIVVRLEIKDSQTQELLLSPKNLYAQLYAVKKVQEGSRVILQDVGLRGTSKLELIDGQAYFSALRFASTSYNNEGVKFHLLMSVYIQNSDTEKPQILNSTISPPIFVDSRKSAGNSEQKKLYLHAEPFTFDLLDKKYFKRENKRKTEQDVEIENDFESLFNYLTAPNIRHKVKHPLFLIIKFSTCVSLYYNPMSVEGNIKIIMEKLQSQLTKDIITKQLSTAGEKQFYLLIEENNDQNKNKKVTEAIDFLNTGCLEIVRKEDQIPSKFIKLGDSAEILENYKQCFDQLLKIFKQLTQKFCESDSNESDDNYSDSEPQSPQKKVKLNEYQNQPSASKMEKLEQEIKKPIIPPEQNPIQLQQQQLTDIVIQQALNQQQQKPIQQQQQQQQQQQSQQQQQQQQQQQLQQQLLLQQQLQQQQQQQQQPTKNKPNDIINKPITTQAPNLTQQNPQLQNKKQVGQPSSSFTAPQPTQQQLQPPSIQHQQPYQKSSHIPGIQPINIPAQGLQGAPQHMNPAFPYQFSQPLDSNALQQVMQYNQLQQLLLLQQQQQLNQQSFPPQTGMMGNPMQSQLLNYMSSLGGLGMNQGQMNPQSLMGQNQNFQQLQQQLLAQQLSSQLGLQAGLPQQLGAGMGMNPLQQQQYLQQQLQQQMGLGSFPNFNPLMGLYQGQQHNIKKPGEGSGIIKQEPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V1N3</accession>
    <name>A0A8S1V1N3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0540224</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8171298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V1N3"/>
    <dbReference type="OrthoDB" id="304289at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="217"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8171298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="276" mass="32740" checksum="C3553978B51DEC6E" modified="2022-10-12" version="1">MDRLNPSSKDCIVPLIEENSPCLQINKPEIDPHFLLPQFTQMMQNPDFFLQTVQLEQIKQMQSSFYSQMNNIPLIQEEPRVMDLFTQQLLQQQLMLNAALLNQFQGIENINYSQTKDLGDNKIKRKHIKKSHNPQFNNGHWSAQEHQVYLTFLQQHRDVMESSELKKTNKIFKLMSDIIKSRSPSQCRSHHQKFNPYSKYLVNNNIVRRKMKEQMLSDSKQQKQSQQENENVEQSQDSNQKQFQQQQQVLNQKMESQQQLMESNVRDDQQQLSGQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V4A6</accession>
    <name>A0A8S1V4A6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">DNA topoisomerase</fullName>
            <ecNumber evidence="3 7">5.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0530179</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the type IA topoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="3 7"/>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V4A6"/>
    <dbReference type="OrthoDB" id="430051at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031422">
        <property type="term" value="C:RecQ family helicase-topoisomerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03362">
        <property type="entry name" value="TOPRIM_TopoIA_TopoIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.290.10:FF:000001">
        <property type="entry name" value="DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.140:FF:000003">
        <property type="entry name" value="DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000380">
        <property type="entry name" value="Topo_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003601">
        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034144">
        <property type="entry name" value="TOPRIM_TopoIII"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390:SF21">
        <property type="entry name" value="DNA TOPOISOMERASE 3-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390">
        <property type="entry name" value="PROKARYOTIC DNA TOPOISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52039">
        <property type="entry name" value="TOPO_IA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Toprim" evidence="9">
        <location>
            <begin position="14"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IA-type catalytic" evidence="10">
        <location>
            <begin position="176"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="615"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="625"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8170672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="873" mass="101861" checksum="61A12F63BE20B267" modified="2022-10-12" version="1">MNNFKTSTKNDKIKVLNVAEKPSVARSIANVLSKDHRIEETKSPFNKLFKLDYRLKDVEVEMWITSVSGHLKNLKYPQKYQNWEKWDPIIILKEAEIENNISNDKQNLELNLKNFASICNRLILWLDCDREGENIAFEVMEVCKESNPNIQVYRAHFSAVTYVDVRRALDTLKYPDENLSNSVIARQEIDLRIGASFTRFQTLLLQKQFNLTSIVSYGPCQIPTLGFVVQRQKEIDSFIKEQFWFIQSEDDQKCIYNWDRTHLFDELIVVLLFERCYQDCATVINVQQKDVQKWKPYPLCTIEFEKLASKKLKISAHKAMELAERLYNKGYISYPRTETNKFPPTINLQTIIRDQQNHPIWGEYATNLINFAFEYPKAGNKDDKAHPPIHPVKMMIESEAQNKTEWDVYQLITRHFLACCSKNAKGSETTITLQINDETFSKTGLVIKEKNYLEIYIYDEWSQSQVPNYKCGDSIKIKLTLQKGSTSPPKPMTEAELIGLMDKNGIGTDATIHEHINTIQDREYAIKRGQIIKPTRIGLALVESYEILGFTLHQPFLRAIMEQRMNEVALGKKDKSQIVQATIDEMTIILKQLQEKQKLIIKTFGQYLEALKNYDEDNNNDDNDGNNNNNNNNNNYYNYNNNQHRIQSDEADETTQNQQRIIKYTKNQKNKKSEDLPKNSTSFPSICQFCNSQCVTNKQLTGNLIKICPKLCQIQEDIPNQSTTQNKDNCFQCKLCQNFMKIRYSKIKKSNFLGCSSYPQCNQAIFLPDIIKSIKIVDKRCAKCESTLFSIQFNENYNQVHQTSFFCLYPGCQQIIKTDWKQQNKDSNQNSFVSKQQNNEKYPFNNHQQNRNFYNKNKSNINKNSLIIKIEKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V4T6</accession>
    <name>A0A8S1V4T6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0570139</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V4T6"/>
    <dbReference type="OrthoDB" id="305783at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8172528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="357" mass="41944" checksum="092B95C3A3C82B72" modified="2022-10-12" version="1">MQFQNHQNQRQSRNRIFDSPISESLSLSRNLSTNFTPVQYDENQKQFIKTSQFSISTPVDQSIQEAQFQQPIQAEIPFGIQNTPQFFKQNNKVIRRELEKYIDESGQLKIDEGQSDEIESSEESLKSFPQNNISNFERKENHKQQFQTTNHENQQEYTLKISDLKSEQILKDSQIQENQLDSNSQRITQNIFSVQKTQIDQQSAQSIQSVKNENQDLSISNVQISQQIHQQSFDQSNISQWSIDQIEILIQGIQVTKYSRKTLFGKKRRILQVSPDLKLIIFRNLEHKITKIYSINKITKMEKGANTETFRLYKPKSDILWLCFSIFLDERTVDLSVKTQEQLRQILDCLYGLIQKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V5V2</accession>
    <name>A0A8S1V5V2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0540147</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8171169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V5V2"/>
    <dbReference type="OrthoDB" id="315136at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8171169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="194" mass="22956" checksum="0C45D9FF684D45F6" modified="2022-10-12" version="1">MNNFHYYRETRIVQKSIKQRSAQKVNQFRASIKSVQPVDSVIYNHQIQREIRIKTDLQLGQELLGTAQNKRFSQLLNQVNEKQQKLTSKVKQERYKKQPTFPPLKGFSQHSQIIEQQLDTPTFRNKKGYLSKTLEIENQSNLIDFQINSFCHPTFDESKQQSPNKEKKNVSFPKCIFFCDVKKARNFFQIPSQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V8H2</accession>
    <name>A0A8S1V8H2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0560184</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000056">
        <property type="protein sequence ID" value="CAD8172229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="309409at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050349">
        <property type="entry name" value="WD_LIS1/nudF_dynein_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44129">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="NACHT" evidence="3">
        <location>
            <begin position="521"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8172229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2972" mass="353817" checksum="47D85CF0B113D1E6" modified="2022-10-12" version="1">MKKNYQNPISQSFDKENTRLNVLNESNNDHQSILYNQINQSYTFQNNINKLRGGGNSQPNPQIQQNRNLNDIFDEQDIFQYLKYHTILLQRILGNQINQNGRDQLEKVVQWINSKGIINQLFNQCRLDERKIAIVMASIDILLSCVINTPIQLSYNLLQFTQNLFQILYYQDKVIKSNIIKNLHIFSDNLQLINQKYQQQKGLEILFNYQFCLIKDVVDHIQDRKAYNLQKCIFEQNTYLKQNDANNTLIQDAKKFLQSDNPRQLDIFYFFQQSKLTIIRKLLNQQQIIEIQQIINYLCKVYQLCIQNNQNWRVHLGFLQMLLELISQKNSFLEDLQKFEGDDITNIMFNELQNQLIEFQIEDNINIWEHYIKSKWDSTIKCYLAFSFMTIKKSQIAKNVESACQKFIDLFLNSDQDENTISVLKNEQLNVIEEAFEQNLKQNQQTYLYKIYGNTQIEKLQEDFINIDLKVQSNINNDDVKKQQQSNKSNYLFREDVDQGVKQEQIYIVNDFLWTENNNTDVLWIKGIAGSGKSYFLKRLQLFLLKHYHSHSKQKKWIPFLISLDALKKLNNNNIWSLILNADDMEFRDEFMGILSDDLENGLVNIVLLFDGLDEMKHQIQEVNLDISLFQKKLGKNVKIIITSRREVQIEKQFIGLQFQNLTEVEILKLDESQINEYLKQYCIENTRQYLYQIYNDFIDQEKNSKISRIMFNEYWQAYFFEEVKTLVEQTNNDKYEIIFTDTLINNFLSKQQKIFQNNNNPKGDLLKIQSSSKLMKIIKENQIFDLIQTIQILQVILQVLPNLNQNITNKLYRKNRFKCILLQLKNEQIKYFNELEQAKLFSEKDYVERLVVILEKNQFFDEFLVEKLDKNIMQISEVDFNLIKNAAKQTQLTKYQIYKLFVSKYYEIYIKKLKDSGFNNINESFTKELNKLQMFLAVQMIKHECLLLDTQQVQNIFQSYLNQNNLEDKKNLILLYSTFITKIQNGSFIFQNKSIQEYFVGKYILKLLKDFSRNFTGKSIEQGLEQSQFNQNEFNLSLLQFSQVINDIKPELLNYEDIKKILLKIIILSRNEVFIRAASNSILLLNFLGIELIGEDLSRIKLSKTNISGLNVYGSNLDESEFNEVKIDYCNFNNTSLQRVKWTQTPWNGVIVFRDQHQEKVSSILFSPNGQFLLSYGSSQVNIWELITQKIFKKIKYNQNLCYWRFSSDSTKFYLGTETFLQIYCIYGNDITLLITIPLQLGQLQLRNFPFTFYNKEEKLVYRRNSNFYSYNLKSKQQWNKSKIADNSIKLTSIKLISNDKILIGTKDREIKIWDGIIKNISKTITKKDHQSEIVSLSEETFDLKINQQEIFDKKRQISLLISASEDQVIVWLNDHGYGYKQVKALHSQTINGQLPRYSIFNKKHLVYIINEYIVLYDLNQINKLEEDITFCDSNCIAFINYEWEQFRLLFIAKENCIEVYEYLNICIGEIPLNQCQPLSMDIITHNLMITYNKDNTIRFWSICSYYQNEEQKFVSNNMDPFRKQNPFSRSRVLQRSIKGFVQNLKQSYKYNENEGTCEVAVQENEKQINIYKVQIFENQIKYHLIKLYEFQEDQKIGCYALCNYGKKICYCVDNIITIKDLQNDDQTLLEQIQEKDIYLIMFSEKEQYLASCSNDNSIVIYRFCVDKKQKFYMIKNKKCLDLFFVKEDDNVLVIQTQTSIIWYKIIWNIDSQQLLVQVFKYHDFEYGVQNGIFDQQMSIVYLIPQQNYNYINLQQVQKEYLAVKQGENIILKPLIVGDQAKVVIFNEQNKKIEFVNEEYIIFSEESSNILEIKNVSDMQVIRKLEYCAFAIREREYLIIAVETIKDIQVIHILPEQIFTTETIKKQIQGKLHHFTLSNSGYFLASTYNQEITIYYITKGPQIIQISKLFIEIEIKDIQISDNQQIIIQSQDGLYQYKLNVEEESIYSIDNYFGNNQLVQNNQSYKIEILIQGNQFIENYDYKDTKLNHNDKLIALKIYDKIYFTSLQRPQLQLILEAKLFCTSIDSKYFAISNDNQIKYYDWQDNIKNIYNFDYKQDLLYLRFLQNPNQILAISSKDIQIIQCQENQIKKIRDGLYQYKLNLEEESIYSIDNYFGNNQLVQNNQSVKIEFQVNQLLEYYNYKDTILNHNDKLIALKIDDLIYFTSLQRPQLQLQLEAKFFCTSIDSKYFAISNNNQIKYYDWQDNIKNIYNFDYKQDLFDLRFLQNPNQILAISSNDIQIIQCQENQIKKIKGRQYASTRASSNLDIAKIEKSIIQISKPEINIELYNPEDYDFNAYNPQFTSDGQYIIYEQNKKVIFQSLFNRHSKYSPEKLEGQYNVSVLPESNYLISFNQQESPLQAYQNCGYRKLEYYLNCWDVSDLSNVENLFTIPILQHQTKKISNKQWFISEIQNNILCLYDIKEFQQGIYDTILHNNNQKIHYSGICPNETKYYTVSTEDWSITIWMMKSCIPLKQFKGHQYQIFFIGFSEDEKYMISRDTDQIIVWNLENEDKLIIKDPNIENQRFLFYCSNFSTFGWNKFNKCDFVKLHHSIGITIQSQGQLFIYDLKDFKSYELFYKLIVHRQEIKSFRHYVYSNGSKIALYQKQQDEKFDKIYILDVKSKQIIDTNNLQRDEIFYGYHNIDKYIKIKIDKEHEFYGLNQKNGYIFVCYKDSNLLSLYKVEDFVNEHGQPIKEVFIHLKIDLNDYNSSFIHLCQVGPKYITYYYNSQLRVIDLERYEKQLKIIKLDKSQEFLNIYKQDYILLKNREEQTLNIYNLRNGQNNKVIDRNIDKMIVAFKNNGEQLALGWDDGSISIYYIYTQVQYDFSIKNKQAIKHILYTQDDKILISCSQDNVISFWEYQYKKEKLIQSIQIDQNIESIVVSPNNLDIALELSGGLIQIITIDKQKCWNQNQKYFGFYKSYPQLRQFLTQNCLVKNSIIQDQNGNSLVHHLGEKMKQEFEAQKIQDEQQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V9P6</accession>
    <name>A0A8S1V9P6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CSC1/OSCA1-like cytosolic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0590195</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000059">
        <property type="protein sequence ID" value="CAD8173577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V9P6"/>
    <dbReference type="OrthoDB" id="293862at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF83">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="365"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="400"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="454"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="492"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="559"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="606"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="636"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="3">
        <location>
            <begin position="184"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="2">
        <location>
            <begin position="357"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8173577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="756" mass="86561" checksum="69F5AFAA87712126" modified="2022-10-12" version="1">MLSQVLQVIGAQQTEEQQMEAIYDDFQRPPKIQIAMMHAAATKFAKTDSNIDLDKTNVCPCCGLPAVIEEIPLCSQRNEFSFNGSGIALYFDFLVFSGIIVFTYIAISCGYNIYVNSHGKRCSHINSSFADQCKTDFFNQFSLTNDHEQLDETRIVILFMTLFYRRHINKIAMELDDAAILASDFSILVENIPRDAKEPEIQEYFSQSFQNETVELRKLCIAYEIQPYLKLNTQKQLKESVLTKVLELEAEGKQIPKSIPSRVQLKQEIDEISKELDYMEDHRATIFGFSGICIISYNYEKQADNVCKTFKATRFQVLLDQLGFEQNQFQQFRNNNLFVRKAPEPGDIIWGNLGITIKEQFKRTLITNAMTLFLLAIGFGLLLALSYLQNVINKNISKGSAAEATIVTFIGFASSILILIINKILAKMIIRFAELEQQATRTDYNISVAYKMGVAQFLNTAILTLIINLFIQENISSLDQAIWQTGGLNSDVMLIFITNSIMPWLTQLIDINYFYKLYVRQKIIKQGENCKYTQNEANKAFEGPSIDLSQKYANLCKTLMFTFLYSALLPIGVCFTFISIICLYWAEKYLLVRRDSKPAPTGSAMAEAMVAFYIELILLLFSLGCTFWEWVNYDKVHVLTWLQLGFSTLYYLIPIKKICGCIVDLGIDNATVESYDEKFLTFYDDYDRRNPVTLDAAKEKWIKIQQETVQPNSQQKTNNQTKQYHKINHIGTVSTKVHPLNTANSREIDDLQQLQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VA88</accession>
    <name>A0A8S1VA88_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0590109</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000059">
        <property type="protein sequence ID" value="CAD8173413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VA88"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8173413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="168" mass="20403" checksum="34C180A926F8ADC3" modified="2022-10-12" version="1">MILQLQKQFKNCILMPISKKIKIQKVSQVQMFSRINIILNFNIQHFQIHSQQYQNHKYLYHLLIAGMQKNLYLKLTQFRFITYYFQAILVKFAFFKNKQLDGSVLIHYNHQAIVIQLNNYVQILRKIIKQFIQVLDIVDHPLFQFIEKNREINKFNGQQNKEQVKKME</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VGE2</accession>
    <name>A0A8S1VGE2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0630098</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VGE2"/>
    <dbReference type="OrthoDB" id="6730379at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="7"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8175523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="241" mass="27912" checksum="5B4A886C01DB4899" modified="2022-10-12" version="1">MSKSNSTKVALVCLKTSQNITNQSLLQVFGKHGKITKILIFEQGPMQTKVFIEYEDPEQAQDAINCLNNTKVMNNIICNVYPSRLRELKLDNVPNTKGMDLTQKLPFSMPSLWDLNVNEDESTLQVSEEKLVDIRKKLSMIDDEISRTIENKLDNALIKLYQQTKCLRVLMGDYQLDEILHKTSKYGKIVYLKQAQQEVYVKYDSDKECLQAYEELKDQMIVQIKYNFESIPGYVMVNSNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VJD6</accession>
    <name>A0A8S1VJD6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine carboxypeptidase S28 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0660043</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000066">
        <property type="protein sequence ID" value="CAD8176871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VJD6"/>
    <dbReference type="OrthoDB" id="330834at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070008">
        <property type="term" value="F:serine-type exopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.980:FF:000005">
        <property type="entry name" value="Clan SC, family S28, unassigned serine peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008758">
        <property type="entry name" value="Peptidase_S28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11010">
        <property type="entry name" value="PROTEASE S28 PRO-X CARBOXYPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11010:SF11">
        <property type="entry name" value="THYMUS-SPECIFIC SERINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05577">
        <property type="entry name" value="Peptidase_S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035712451" description="Serine carboxypeptidase S28 family protein" evidence="5">
        <location>
            <begin position="22"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8176871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="462" mass="53632" checksum="21012A948C556031" modified="2022-10-12" version="1">MYLILSALTICSYAIAPHVLTQGPFTETKQTFQFTQLLDHSDPANNQTWKQRYHVYSQYFNPTKGGVILYICGEWNCQGVGDNSFSFQLAKDLGAIVIALEHRFYGQSQPFGEDSWSLENLSYLNVHQALDDLAYFILQMKRLQLHNIDSTLPWYAIGGSYPGALSAWFRYKYPHLTVGNLASSGVINTILDFWQFDDQIRKSTSKSGEQCPLYLQLLNSYVDKNLKNFNTKQAFKESYRCGKMTDNEFRWFWADTIVQMIQQGQRTEFCQTLESLPSVERMAEYIREISLSQGDSYKQYGAYYLRNETIDENSQNRQWYFQCCTEVAYLQTPPQNKNSLRSYELTLDWWREWCNDAYSQGELIWPDVRVTEAYFGGLNLNVDHLIMTNGGEDPWQRASLTNKTKTNSKVITYLIDCDDCAHCVDLGAPSVNDPAILTQTRQTIKNTFKQWHDQFWSKILIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VK52</accession>
    <name>A0A8S1VK52_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine/threonine specific protein phosphatases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0640188</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8176139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VK52"/>
    <dbReference type="OrthoDB" id="282592at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033192">
        <property type="term" value="F:calmodulin-dependent protein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097720">
        <property type="term" value="P:calcineurin-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043360">
        <property type="entry name" value="PP2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45673">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2B CATALYTIC SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="1">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8176139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="332" mass="38317" checksum="09EE041AEF01BD77" modified="2022-10-12" version="1">MDSFDLFPLACVVNNKFLAIHGGISPDLKTLEDIKKIDRYHEPPRSGLFCDILWSDPVDNDQGSLENGWRGNEVRGCSWFFGVDAVHRFLQRNNLISVIRAHEAQLDGYKMHRWKGSSDFPVVITIFSAPNYCDVYKNKGAVIKFENNTLNIQQFKDTPHPYLLPNFMDVFSWSIPFVTEKVTEMLYMLLRHEDQDQESGDEKINQEDIEKFKQITQSEKIKNFEKNKSLQKQLSKSGKEKLRSRLKFVATMMKMQKTLREENESIVQLKGACPDKRLPKGLLLQGKEAITDALQEFNYMKLADSINEKMPNIHIPQQSIQIKKPGQSSKKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VKL4</accession>
    <name>A0A8S1VKL4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0670167</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000067">
        <property type="protein sequence ID" value="CAD8177377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VKL4"/>
    <dbReference type="OrthoDB" id="307910at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="824"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="725"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="738"/>
            <end position="757"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8177377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="902" mass="105872" checksum="A57D70554257D52E" modified="2022-10-12" version="1">MGACSSSKPITKQSNQASNRQIQEDPIISVVNYILEEPAQVEIENSKQENLNQVNQDQDNQYQENQLSDIDHLEINEEDNHNFIDEEILLDNTDDTIQKPADTVNFINTGQTLMQHRNQSDVKVLQKQRIFQTDIETQANQLLESVYQRKSDMITSYSKLKRQEEALDLGFASVGFPDESMDCEAVYEILQPGFIFEVIVNKKNIVKGRLDVILQKFLAFSDKCITSQHQVRSADFQQFKKQKHLLSQLTQINVMRMKGVIFEQDVIEDFSIFTYYINFEKIQVGDHLIAVLIDKHKMTMSLKNKSIMGGKQWSLKSLQNPSDFDNWLYFRLGYAKQIGVNFQYQSMNEEEKKKAQEQINANLKFKEETGILKMDYQIEQDGFFPNKQNYPFMMQSLGLSQNTSFTNRNNLKSFMKINLDHRRRLYQIIYFFQTFKSICLARCPIDLCSQVEESKQQLKNNKIIILITMNSSYQYNLLSCNQINIFYQFIMLPKFQNSFSLDQNLQTLIESFNQSQKNPGAISRNRISNNYPCIPSAIRQSPGSQTLNLFLEKVTFILLSNSAINTTFLNDQQDIILKLLFNNKILQLVIIDRPLREQKEDDQSFNLYLQSVYPYQQQHKLKVDFKLEDSDEIEVNGNDNVVTILTKAQIKQQKNEQNKKQIQCVHQDITNKAETQIVQIQFKFLREWYLNLGNNIFAAKKALQEYFKSLSIPKIKFTAFDNQAPSTEQPIIPPQQDLNKKQSLKTEVKQEPEKEQEGVEQPPQIPEKDLQKLFPLVSEEFVQRQRETLDKKNCTSEEKIKRLFEEVRIKQFKQQTYSADKNFFKCPYHKCQKGYKRPNQLKNHLKQNHQKLSEIGFTIDDNGEFKYNERILDYCLLLWKVYPNFVKSVINEMRSRKEQQSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VM59</accession>
    <name>A0A8S1VM59_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0690085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VM59"/>
    <dbReference type="OrthoDB" id="286600at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8178280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="500" mass="59160" checksum="621EDE143D8330E2" modified="2022-10-12" version="1">MKKLKSKLESTNTDSFGISRTDFTRTQKSHRRYLVNTSIKFKTSSSELNRMELQEEEDQKIVNPIYLNKIRQQKKSQNTFKRTDYFHLKKIPSINSQDICQKSNRLQQLSSCIIRCYSDEIASLKDIQTIDDIEFDNYVSRYLNEASISIDILIKKLQYLQQYKQNREKSEQEMKEIMQECFKQKNVCIQQRFCYIFGKMEQLFFNPISGAIYFKLCKRLSDNEQYYRNKMKAYRGLGECLLRVRPKLSQLYFTKYLMSAWKLNEKNHELYAYDLLGKYYFYVGQIEKAKQFHEKMIGGYCEVPDSRVRVLAQSRLEQGSLSNRINREHQVVDIDVVTSDDECYEIVLTQPQPVQVTFVGSSKYFQRKIPDPKIVKEEANTNIRQKKPKFNSQILESGGQFDMSKLVISNPHLNIGVIKDRVLLSHMTPNRKLEMYQYLCLANDKNAFNNVNHLSGLYDKFEISKINKYLNKLISLMNIVQQWLINQQRVKTNIKKYPLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VQC6</accession>
    <name>A0A8S1VQC6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0680131</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000068">
        <property type="protein sequence ID" value="CAD8177842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="297598at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057352">
        <property type="entry name" value="TPR_TmcB/C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25474">
        <property type="entry name" value="TPR_TmcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="95"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="180"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="225"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="286"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="309"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1059"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1246"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1354"/>
            <end position="1375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1588"/>
            <end position="1609"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="3">
        <location>
            <begin position="607"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1004"/>
            <end position="1032"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8177842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1649" mass="193218" checksum="98E0BB598B54B18C" modified="2022-10-12" version="1">MKRTSLFEDDKTNIDKIQHSFKIMFFEIVHNLILGDNFPFFIYVIFILIESLQVFHFFFIDEFSSLWKVKSWTSTLNSFFSYFMIQPYLKNLSFQTYILVNYIVMGLIMLLLIVVLFIALKSASLGRMTGTLMILKIFFEVLNYVLFMPILQLFLTIFNCDNTTHIHKIYTDQQCYNGNYLLHVLLCSIAVIILIAVSTLVTLMFFESRFQINNPQCKITGREDLIFVIFKVVIVLCFTILDVQNQRLLIIIILTLFSIKLFHSFNKSSIYLNIYFAKLLNGQHMIILWTMMMVVLGLILENTDYDGAPQLWVICTPLLLIIILVRKEYRYEVMMSDSNKFDSLDQSLQLLQYLTKLVGYYDKDESIASLLDGYVEYHKVTCKRDDCPCSPKNMNDKRIEKFKKGVKDLQMKNQYVILIYIVEKTYVLSLTRYPNCIELRISHALFLFEKMQSTIQALQELALADQEKPYLDQQFLVFRLRKIIEEQMFDSGNLTNVNEFTAENNLREIKMLIEKSTQLHLDFWIKLREDTPDLGKLYEVGLRMLYVDKVLDDQWKRIMKINFDLPPTLLMMYSRYLIDVLNDREEAEDVVERLKNLQTLEMDRGKIINNLKDFVNESTALISISAEDGNFSRILGLNQSCAKMFGYTKSELLDRKINVLMPNIYAQYHDQFLEGYTYSQESRVINKERLLIGKHKSGYIFPFFIYIRYIPSLINGSQFFAAIKQERYFKNQAYMIVNCFDMCIENISAQFISLFHIDLNYISKKKTQVSDLIPQFEEFKQELMSKQGYETTVQYKSRDQFIQQQFQVYVSEIIICVNRFINNQNKEGNLVQTNLTLNVPQSQHHKKDERQAQLFGYIIKLEQIRNEKSILQTPSEYGLASTRKQLNSNQKFLFQFDSKEIPGMKERIEQQKKQYRKDNNVKLPDQFIYKFVGEYISDQNFQMSAISISKQDETYEFSQKNDDDKTPSSKVEDKNNDLSLGIKTMILIGGQLFDSDEYKNMDFEEEEDGQSKIVGQMPSKQEEDQEQENGNTNVYKSKKTFVQFLKESRSANQSSLTCFRWNAALLIIFLGVLGLLNYILSQQLFLQVYDGYMNVINSNQRVALGQRILWQTVELQLLNQLNPFVDQTKRIAEQRQGLNQSISNFQELQFILQSAVVEGYQIDLIRTNSVKMTGRDQSGTLSTQLVDLNQATAQILSKALELLQEYNPESQFFISYNLLNNYFDAVVQSANIYGSQLKEKLNESSIIITLFIVACTFAVVSVPLITCMFSLVSQNQEIIMKLFLQIQINKVKKLVNICEGYFNSLQVGEEDDEIHRDNEGFMDDDEVQDEDEEYLERHKKRKKYKYDLKNKRNFYLQFAISMVMLLGYFLTHFLIGDYLQNAFRSLVDEIEATSQLVPNITFSNNVIRQMIIDPNFEVQGNSSYLISSTFVKELYDLNTKIQKEHSSNIQYHNPTYSTHFDSLMKSGACQYLFQIIDIDINICQSFIEGIIDQSLSLALSRYFEDIRSQLQEYHNIMKDPSYIISDTLYNFTSNSTYNKALSLLYTNRQTHEINTLQDLYLRQIFSFTTDLFLQCVQKDVDTNTTTSVAIFIVFLVVLVLIYLFFWWPTATKINNEIKKTTLLLANIPLSLIKRNKAIREYLNRMHEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VRA8</accession>
    <name>A0A8S1VRA8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0710166</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000071">
        <property type="protein sequence ID" value="CAD8179271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VRA8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8179271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="302" mass="35754" checksum="0BCB00DB57EFA7E5" modified="2022-10-12" version="1">MVHIDLKYSFHTYPIKKVVAADQINLQKFLTTVKGQTRLVNYIPNQLDQEVQESKINKLKKNLKTTQQLYFNFYLRDHHLAIFKLLIYNKAEAVGFIKSKCRENYRINSTQRLESFNINLIFSIKFNMINGMRKNSQIYLIYWFFYWQPKIGYFANHGHIQIIMRFLYYFKVALILSVPSISGELPALKAQIMAYTNLLFLNYLKFRPQDVESQFAQILIVIRQQIKIVLKKCHSYQVLLFYQLQYSLLIEYLISQKERQNGMIPSQLKKGLARNLLNYDCSLIKGIDQIYGLPTETTSIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VW05</accession>
    <name>A0A8S1VW05_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0750063</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000075">
        <property type="protein sequence ID" value="CAD8180837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VW05"/>
    <dbReference type="OrthoDB" id="304203at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="143"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="283"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="322"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="398"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="424"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8180837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="478" mass="53922" checksum="71BB3C3FFB7329C7" modified="2022-10-12" version="1">MSLQELEKSLELQDLTQAFIYKDLISPSIANAFCYTLQQMPAAITIYFLEQTQNQLHVATFGVGTIFYQAFGFSVMNGIASGLQALASQAMGAQKFQFCGKLYYQSIFICLILIIPVAIILYSSEDIMLFIKIDQELAEATGLLNKGMISVILFEGIFAQTKAFLNAQNLYHLSVYTHFTSCFLSLLFNYFFISILEMELLGGIIARNLLEISNNLIVVYLIKRLNCCKETLIKFEWKLLDGSFKFFKTALPIGSIFWYESICFELFQIQVNYLPSLLLSLNVLMYTVALLVYQFSYGISIAATTFAGNAMGAKSAKLAKQYTIGSFVLLGGFLIICLILIIGFQQQIIQFLTSDVEMEELFIKFLPVLLIQIVADGVQAIVSGIVKVIGKEHFASQSMMFCYIVFGQPVAYIMTYVLDYELAGVWWGMIVAASTYSFLQIYIMLKSDWHHLSNEINDKIHSMGAHHIETELQLLDKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W0N1</accession>
    <name>A0A8S1W0N1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-actin-capping protein subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0820027</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000082">
        <property type="protein sequence ID" value="CAD8183878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W0N1"/>
    <dbReference type="OrthoDB" id="310148at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008290">
        <property type="term" value="C:F-actin capping protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001698">
        <property type="entry name" value="CAPZB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10619">
        <property type="entry name" value="F-ACTIN-CAPPING PROTEIN SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10619:SF0">
        <property type="entry name" value="F-ACTIN-CAPPING PROTEIN SUBUNIT BETA ISOFORMS 1 AND 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01115">
        <property type="entry name" value="F_actin_cap_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0117">Actin capping</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8183878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="505" mass="58772" checksum="80CBDD053614A66C" modified="2022-10-12" version="1">MESKEKILSCLNILKRLPPTQMQKNAAALASLIPDYAEELYQKIDKPLDIGQDEKGNQFIQSEFNRDGDSFRSHVTNQYYPPIDDAVYPSEALRKLEIKANAVFDEYKRLYYEGGLSSCYFWDKEDGGFATAWLIRKNVEKSKGIEDGSWSSINVIDIKTDGKSKWTYKITTSVVLEMNIVQNQDVGKFNITGTLTKQKEESYEAPAGNKDLELFHIMKIGTLVEDVESYLRSQLDSVYFGKTKDIGIGRRIQIEIWMIQLNSIMNFIKNNIKPFLLTSGIFGISGYGYLYYTAYQNKKELQIKKENEVEFKDNTRPQNQYFGLSYGFRADQIVLDRLDSGDLVFMKFECMECISVSDIIKCEYSQFTKPYPHYDSLGIIFRDNRNVYVLYNRFGDIVIEDYAEFLQNPFWNEITIRKIITKHEIHKTLKPLFYNEDYKQLFANKPYIPIVLKSTGLIEQKYQDNLDQVYIEDLDVDEFPYFINGTSLGPKINVRTKRNKDLDAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W1R8</accession>
    <name>A0A8S1W1R8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA repair nuclease/redox regulator APEX1</fullName>
            <shortName evidence="8">APEN</shortName>
            <shortName evidence="8">REF-1</shortName>
            <ecNumber evidence="8">3.1.11.2</ecNumber>
            <ecNumber evidence="8">3.1.21.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">APEX nuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Apurinic-apyrimidinic endonuclease 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Redox factor-1</fullName>
        </alternativeName>
        <component>
            <recommendedName>
                <fullName evidence="8">DNA repair nuclease/redox regulator APEX1, mitochondrial</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0780082</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="6 8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="6 8">Probably binds two magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.2" evidence="8"/>
    <dbReference type="EC" id="3.1.21.-" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W1R8"/>
    <dbReference type="OrthoDB" id="498125at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09087">
        <property type="entry name" value="Ape1-like_AP-endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000130">
        <property type="entry name" value="DNA-(apurinic or apyrimidinic site) lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020848">
        <property type="entry name" value="AP_endonuclease_F1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00195">
        <property type="entry name" value="exoDNase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00633">
        <property type="entry name" value="xth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF6">
        <property type="entry name" value="DNA-(APURINIC OR APYRIMIDINIC SITE) ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00727">
        <property type="entry name" value="AP_NUCLEASE_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51435">
        <property type="entry name" value="AP_NUCLEASE_F1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 6 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="10">
        <location>
            <begin position="91"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="38"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="5">
        <location>
            <position position="239"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="7">
        <location>
            <position position="310"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA substrate" evidence="7">
        <location>
            <position position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8182215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="348" mass="40082" checksum="4501C55A5DF2F692" modified="2022-10-12" version="1">MDQRRGRSGKKKNSPEKNDEAQEEFVVKSKGKQQKGTKQTEDNEEKKGVQKIEKQQNIKEVEKKVKQINQAEQKYELGDGKFSTPTIKISSWNINGIRAVTKHEEAVQYLSDNKFDVICLNELKADQIVFDKENLGAKFGKHYFLYLNFCKSKGGYSGVAIASKVKPISVKCDIGVDKHDQEGRTLTAEFEKFYLVSCYVPNAGQKLERLDYRTKEWDVDFQNYLEGLRKKKPTILCGDLNVSHHEIDLANPTGNKKTAGFTQQERDQFSNYLQKGWVDSFRHLHPKEVKYSFFSARNNCRQENKGWRLDYFIINKEASDAIIVSDINLTVHGSDHVPIECEVDLRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W2A3</accession>
    <name>A0A8S1W2A3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T0820156</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000082">
        <property type="protein sequence ID" value="CAD8184134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W2A3"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05599">
        <property type="entry name" value="STKc_NDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000570">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000192">
        <property type="entry name" value="Serine/threonine-protein kinase cot-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050839">
        <property type="entry name" value="Rho-assoc_Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988:SF76">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_135, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988">
        <property type="entry name" value="MYOTONIC DYSTROPHY S/T KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="83"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="13">
        <location>
            <begin position="362"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="440"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="491"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="451"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="502"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8184134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="512" mass="59881" checksum="AE342B3A74447AB9" modified="2022-10-12" version="1">MAEVKLSNQTKDRVAACKSYIERKYQLSLQQEMEKKMIWQQLQQNMQNLNFTPIEQELIKKEILHKEAIQLRKKRQKITVMDFEPIAIIGRGAFGEVRVCRDKETKEIVAIKKMKKSEMIFKNQLGHIRAERDILVQANCPWVVQLKYSFQDEKYLYLVMEFLSGGDFMTLLIKKDIIPEKEAKFYTAEIVLAIEAVHKLNYIHRDLKPDNILIDENGHLKLSDFGLCKHLGNKQNEILSIPYTERKQDTQQQQTRRQLAFSTVGTPDYIAPEVFSQKGYNDLVDWWSVGVILFEMVIGYPPFYSDTPQKTCQKIVNWKKHFAIPKEPKISPACADLIKRLMADSTERLGDVNLIKKHPFFNGIDWDNIRNIKAPYLPDKSKITANFDKFDEQDPWYQTTDKKGREQNHFQGYTYKRTHENEVSPIKRALEELENIKPSGIRANFDKKQRSQSPVYNASPHLNQGQRSQSPSIKEQLKQTYVQYFGQYLSPLNKQKRPETAQIKSQVANKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W2S3</accession>
    <name>A0A8S1W2S3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ShKT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0810015</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000081">
        <property type="protein sequence ID" value="CAD8183471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="301824at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002895">
        <property type="entry name" value="Paramecium_SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003582">
        <property type="entry name" value="ShKT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01508">
        <property type="entry name" value="Paramecium_SA"/>
        <property type="match status" value="29"/>
    </dbReference>
    <dbReference type="SMART" id="SM00639">
        <property type="entry name" value="PSA"/>
        <property type="match status" value="33"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51670">
        <property type="entry name" value="SHKT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035920105" description="ShKT domain-containing protein" evidence="3">
        <location>
            <begin position="18"/>
            <end position="2691"/>
        </location>
    </feature>
    <feature type="domain" description="ShKT" evidence="4">
        <location>
            <begin position="754"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2648"/>
            <end position="2671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8183471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2691" mass="291691" checksum="512503229D223C4F" modified="2022-10-12" version="1">MNKKIFMLSMMILLIESQFSREKCTCQELLSEDDCNYNILTKCSWDGKNSKCNIFTTTDNPIKKYLQYCDSYNENDCSQKLGCAWLSNKCILFTGCTAYAKKDNSECQAINRRCISDGTHCVEIDACNTYKKQISCVKNANKKLCYWNSQNNSCVDASTCDKLPKIFAIDKECRDVISICTVIPSGGCVDSGNECSDQKLEIQCFWNKLKTTACYWDGFACKDRICENAPSSFTTDDACIKFRTDGTCTTQANGGCVTRTTCADAKIEESCIKNSSGDDCYWTGTTCFDKTCVNAPITMTTNSACAGFVTGCITKSGGGCVANGACSVANVQAACVKNSSNFDCIWDTTCKEKTCANAPTTNNTHDLCTSYLSTCTVKSGGGCQNRTCANAPKTMTTNDACEAYLTGNNCITKSGGGCVTNTTCAAITLEAACVKNSSASTCFWDTASSSCKDKTCLNAPATNTTHDLCQAFLNTCTVNSIYAGCVEKTCENSLAQSYCDNDTSNRTCIWKGKCYKKQCVFASSFIKTHADCQAYHSSCTLSNSGTGCVPLPLKCEAITIEAACQMKSNKKPCGWNGSQCIDKGCSTASKTFTTTTQCNEYLSICVANNPIIVNGVSKIEGCQDLPKSCTSRKSVENCEISRFGYPTCLWVSFTEQCVEKSCATASNLETAGALQGEFTFSGCQAYLNTCISNNSADGCIVKPSSCSSLVQSNCYIGSKVGGDCYWNGNSCVDRICSNIIQTTHANCNAVYYPCTVNNLGTACQSLATNCTSYLSQENCKITEKYEYCIWTGFSCRNATCADAPDSSSYDSDSECFTYPTPSEKCTVIYKVGGQGCVKKQANCSDYKTQTQCHKTITNLTVDDDCKWVIDKCYELSIFTFGTCRQFKGTQRMCQEYKDGCTNISSAESSVTCTLDCNLKRGSGLTFADCQALDYTCSVKKDGSGCIYIQSTCFGYESSIINCFRSSASGNAGYCVMNDFSQCQSVNQASECRYVTGLIDMDHRKCQLYHFDCTALNDGTGCQEYKRACSDYETANNCSISAQGKCFFDGIYCVRFSNCASIIGIGLNNIICSTYHPGCTANANGTACQEKMPVCDLYFMQNSCTISQAVATADKCVWTGTQCITITNVATDCTYVTGYELTDPICASYHPDCTANRQGTACQERLTTCEQYITQFNCSTSTFYKCVWTGNQCIHVTNVATDCAYITGSKLTYPICASYHPDCTANRQRTACQERLTTCEQYITQFNCSTSTDTVTFECVWTGNQCIHVTNVATDCAYITENGLTDTICASYHPDCTANRQGTACQEKLTTCEYYITYFNCSTSTFYKCVWTGNQCIHVTNVSTDCAYITGSKLTDPICASYHPDCTANRQGTACQEKLATCEQYITQFNCSTSSFYKCVWTGNQCISVTNVVTDCVYITGNELTDTLCATYHPGCTSLKDGTGCQEAKPNCKDYTSFNKCFLQTTALPSCIWIDNSCYPITDANCSVITGYGLDHAQCQAYSTGCTSISDGSKCQDFKSTCEQYPGTTLGCTKTYPQKCYLYGSSCIAIQNVETDCAKITGSAGAITYEICQLYNVGCSANRARSACVQLQAQCSGYTSAMTSCYKSRAGLCIASTNTDTACVAASTATTCDVVYLGAGNYSSANCNEMKAGCTNNGTTACVAKTCVNAVVTFNHPNCNNYLNTCTVNSGNSACQTIASKCADQTQVSCQYSFEGECVVVGTSCVRKTCDNAAIDVTRDDDIECSTYLQSCTVARLGGCQARAACSSYISSLQCKFNTSGGKCFWNPTLRICVDLNCVNIEATTLFDTHNECVVVDEILACTVRARDGAAIQGCMARGACSSYTIEEQCKTNASGDLCVWNTNANLLAPACQDKSCTTASTSTTTHYDCYAYYETATVKCTVFATPSNSGGNPILGGCQQTTDCSNYIDIEQCQINANGDPCVWNGTSCADKSCATAPIDYDDDTKCRTYITNKCTVSDSGYGCVDIPVTCETMKQNQCYYNKAGDPCYWTGIECIKRSCENAPDTTLTADDCNTYLTGCTLDNIKCKTKICEDFEFATDYLCKQSISTCTTNGKNCVTRGSCLQALDKSGCILSSSGEQCEWIPDDDINSAYCTIKTCSTASDSLITEEQCQQYFRNCTTKKGGGCVEKSSCANANVESACTTAFNGQICVWDSIKQLCRDYDCQDIDGTLHDACQSRKSDCTAGINGRCAKIQNCEQTNVRAACIVGLNGPCLWIDQYLNQDGSKGACFQYTSCQSLSWKTDQQCKQINDQCTTDGSKCVAITLCSETNINGGCVTGYDGLCVQSVLELNSLEPAVCQPYKSCEKVFYITHEQCQQANGNCTSNGKTGCIDLTECSSYQSQLGCVINKTGLIVQSGIVKSTGLCTWDAKNNQCRDQDCSDYIRTTHRECFEILSICTSNGETCIKQEVCSSYSTQVACETAVGSDGICFWEQASLKNNNNAKCRLLICQDIQNSLSNNVCLASLLSCVIDNSNICIPKANCSTYTSKSACNYGGLDGICIFKQQTGIDSISGIGTCTLMRDCSAANDDQNACQAAKDRCLWIPELIIQGLVTPSKCSALDCTLNWAKNGYCNRILNWDQTSQQICKLTNGTCVAINPITLSSSECLKISGYTYTWNASKNQCQVCKQPKPNSSQNNDTEEEEKNENTESTSSNQILSIIGIMVIDYLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W847</accession>
    <name>A0A8S1W847_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0840114</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8185003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W847"/>
    <dbReference type="OrthoDB" id="425067at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000457">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF5">
        <property type="entry name" value="MAJOR FACILITATOR-TYPE TRANSPORTER HXNZ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="182"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="304"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="374"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="397"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="426"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="457"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="58"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8185003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="491" mass="55711" checksum="EEA994625102BDF2" modified="2022-10-12" version="1">MNEIEQQEASHYSTINELKSEIQVVSQKEDHSIIKSDNIINFDDTLTQIGFGRFQIMQYLVSIILGIMEGAQITIFTLMIPILKKEWDISESIVSLQTSFIFMGFLVGSMLSGQFTDRYGRKLPFIFSSLFTVLICFATILCTNVYQLLIMRGFMGVFVGFFAPCCVTLLQEITPNNLRGQMTGTVTLSVAIGQLYGFFMASLILDGINGSWRWLTFLGSLPGLIALILSLMYIQESPRYLLLEGKYYDAFQTLRLMIKTNKATEINLSIEIEHKLVIWSLYMNSIAKKTEHASFKSLLNGDRLYISIVLWSIWFLLCFSYYGNLMTMPQILYQLKDNQSQLQQLVYACLSDILGAILATLIIDIKGLGRKNSLIIGFLIAAIFSFLQLYYYEKHFAILAILQKMFLSMNYIFCYQLTTELYPTKLRTTGLGTAVAIGRLGVILMPWSCMIVSQYSIIAPFLLFSIASFLGSIFTCFIPFDTLGKSLDNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W8M1</accession>
    <name>A0A8S1W8M1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0870008</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000087">
        <property type="protein sequence ID" value="CAD8186324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W8M1"/>
    <dbReference type="OrthoDB" id="431454at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="171"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="273"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="307"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8186324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="357" mass="42107" checksum="7D375E343ED16F79" modified="2022-10-12" version="1">MNQYHDVTIKCPNTEHTQNVTLVCFDESCKGERLYCHQCIKNGNHISHPQNQEELPFLFEHIQRVEKECADLIHSMNQQFDLVNHDFQLLIDGIRSKYQITKQQLLNLNSKQINSYLSQSIHFRQFEFTTIQLVYQLTNEIKSQIQKLLSDLQLNELNYYQFSKLNLNKSEELYQRGYKLYLNDKKFDEAIKIFDQALALNSRHQLSLWCKADSLKMLGYYNEAIIWTDKALQLDPRHYNSLYSKAESLKMLGLFNEAILWADKALEVDPKHCHSLYTKGDSLRLLKKYNEAMKVIDYSLQINPNHLDSLRSKGACLQDQKHYQEALIFYSKALAINSNHEWTKNRKNECLKALEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WAI3</accession>
    <name>A0A8S1WAI3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0880119</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8187044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WAI3"/>
    <dbReference type="OrthoDB" id="291504at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8187044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="263" mass="30892" checksum="3BDB605BF1CF8E08" modified="2022-10-12" version="1">MSNCIIKEEEVNVHTPVSISKTTLYKLNKKKDLEENLPFQIAYSKAVSKFLQALHILQLAQNHSQNISVPSYPFVLQYSYNEFLVHLGGHKINVQNTPILNDFQQQLHIQQCVPRLISQQSPSIKLTIQKRKGISNQEQKDTKNIPKNYCKAIITFASKHQTLCHQILGDQLKVVKFLDRITVYKKKLMNIRTFSGLLSQSEDPIEEEFNKTFRILSRIFIKKYAINYIFNQKIVQHNWHLRYRQQIYKGIKNPKNFSHIKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WCK9</accession>
    <name>A0A8S1WCK9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0870260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000087">
        <property type="protein sequence ID" value="CAD8186803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WCK9"/>
    <dbReference type="OrthoDB" id="304338at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8186803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="18459" checksum="E5960203D5C3F4F9" modified="2022-10-12" version="1">MKQIKLIDNLQAFTIDYKITVRHALFLSYGMYLTYQYYSQMRTCEDQIRDELLNQKSQIQQQYQQLYSKSYEGILDCTLDKGDWIFSTENPKIDEYFNQKLSLNYQYQTIMPLYLCAGIGFPSLAILCSYLQNRDNRFIYGLAAAALFSGIKLIIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WHC4</accession>
    <name>A0A8S1WHC4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0910106</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000091">
        <property type="protein sequence ID" value="CAD8188371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WHC4"/>
    <dbReference type="OrthoDB" id="310228at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="313"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="69"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="171"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="282"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="326"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="350"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8188371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="361" mass="43152" checksum="A6E997C1E24A462A" modified="2022-10-12" version="1">MDQYQNAFQMLGQNMFSKAPPSGYGNGYQPMIQANSNKQKFDQLIQLMQFQEQTVSDYNTRMKGKEDHIYSLEQQSMQLQRELVQKDKQRNEHNQINYKNYQPTFNVQNTLPIESLQTDLQSVQNQNQNNLLQAVDNPEVQPQQIQRRTSIQSNQNQTRPQSYAIQGNQKLLNMQKQMEEQNQYIQQLSALVSKKKPVIDSDKDDSLTQLLNERDDLQKQQILNEIKQLKQRVDEFDHSRQQMIPQQFQQQGFMPQQQLMQQQGMMQFQNPYGMYPQMYPQNIQQQQQLQQQNDELDDNEMNNEILMKLIDQQNRQKKRGSRNRQNSHDYNHRDQRKDRRNTQKRKQQQDDDDQNNDGDSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WI52</accession>
    <name>A0A8S1WI52_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0910068</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000091">
        <property type="protein sequence ID" value="CAD8188297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WI52"/>
    <dbReference type="OrthoDB" id="286734at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030258">
        <property type="term" value="P:lipid modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049941">
        <property type="entry name" value="LPLAT_7/PORCN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906:SF4">
        <property type="entry name" value="LYSOPHOSPHOLIPID ACYLTRANSFERASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906">
        <property type="entry name" value="PORCUPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="363"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="402"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="433"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8188297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="461" mass="54576" checksum="2C96683AFB0D299F" modified="2022-10-12" version="1">MLLSFFEWLGGLIGLPEDQARMVFGFIITIPLGFLFKTLKSAQLRKYFGLTIGLLLSYMLYQEKLISVIIQNIIVYEMTKRISLNKIGIKAKWVFYETLIYVAVHHIYRQYTDYGGWKLDITTILMMNTVKWTSFAYNYEDGLRKDEDLNSDQQERKLSKMPSYTDYFGFMFFFCGCLAGPSFDYYDYDIFIKRTNVYQNIPSTFSETFRLFRNALIYSFFIVVVYPRWSLESLLSTEYEEACLLYKILWLNLTITLHRTKYVSGWLFSESGLAAAGFTYNGQQDGKDRWLRIRSIDPTIDLTTNPKDKIELWNISVQVWLKKYVYLRVYPESVLKSSAAKSQKAQLITFAVSAFWHGFYPGYYISFFHWNLIGMINKYIHKLGQNTNVLKIWDKNIFFRSVRFYFVNSLFNSFGVGFQLMNVRDNLRFYGSVYYIFNLTTYILFAFFTVTQFGQKPRKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-06-18" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WIN3</accession>
    <name>A0A8S1WIN3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0900202</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8188089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="270970at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007009">
        <property type="term" value="P:plasma membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012968">
        <property type="entry name" value="FerIin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037721">
        <property type="entry name" value="Ferlin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12546">
        <property type="entry name" value="FER-1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12546:SF33">
        <property type="entry name" value="SPERM VESICLE FUSION PROTEIN FER-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01202">
        <property type="entry name" value="FerI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1523"/>
            <end position="1545"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="8">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="8">
        <location>
            <begin position="183"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="8">
        <location>
            <begin position="1321"/>
            <end position="1448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="855"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="858"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="883"/>
            <end position="900"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8188089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1552" mass="177978" checksum="BAB051AA7447E31C" modified="2022-10-12" version="1">MKNKQQQRQEYLVQVQVIEVRDLKGGDKTGACDPFVKISVGNLPPQVTTTRKAANTAVFNQSFTFTGLLMNSIEFESFEIKIEVYDLKQFGQNQLIGIYSVGISTLYRNPAHEIFNTWLPLVHSKQGLEPQGFLLASAYVISPTDRPPVHDMNENNVEEEPDEFGGVPDDQLNPEQLQARIERKNRIAVVSKPEIRGKSYQLMVNIAKAEDLALMGYPTLDSFISVRAGGTAQITSVMKNQQKPQYLSRLMFPLHFPFFNDKIVVRVWDKRTMATDTFIAMIPEVPTENDYFNINFLQSKGGTMPFRWFNLYGIPQDERPGGFQQAFLGYKKSIIGTDFMGRVLMSINLTQSEKPELMVVPLTTQREPPIDKYLLRFDTYEIQEAKDCGEKIIVKFKVGGLVFKSNEAYIKTEKKSKYKQKAQSENKEIQGITYTWGKQIQTKDEKLDLPADPQQIPDLIVSLHNEKGKRVAYLRIPYSDRDLEINRPKWYTFKSVKQTDNNDIPHSFFLVNIIFRNESTVFKIPKIIVKRDQQVYYHLYAYFYAGYDLCPDLYSEEVQGHFVMKIGGQYLELDKLQQGKNPIWNKAFEKIVKIDQNLEFASNIIITFTNQKKTGSWFSNNHIGDICIPALVCKYEGDPIFAFHHIVNQGQSAGRILAAFKLKELGKNKEAYSEQPTPIIPQEGFTQKWAQFPVFGVRNLKQKMKDPKLIVQVPIPFNMVDPTIENASYYNSEPCDIIQAQKDLLENDKSWFGGGIQDPVFLKLVNLKVYLPEDIKFYPQLEIQLSDSFNKNERYICSIPLIDVAQTKDQILIDEARNFFEQGRNFDDIQLNAGKNEDLQEDNENVVVNNDVASQKQLDQKDTIMPKETQPKETIKQSIAPEKMSQQKQSNQSGKSQKYSTMSKLNVMSDRPDLKIKSNLKNKGIQNVEVTEMTHLTLELEDIIEKKKENREKEKKLLGMIKTYKTKYKKFDQLKLLIKREVEKLKGVEIKETNYFKGTNEVDEDEGYDYGREVLKGPYEEKLAPKIPFRRFALYRLSTTKYGLIGNPTDAIIKGDIRISDHCGTIIDNQKLSKKSKMSKTSKKKSSVKDVPEKPDDYSFNLFDEGFLQFFNQPYRLKVRLYILRCLNLAAQKQDIDAYHKMAGLSAVCSADSYPEIFIGESGHDNQDLIKHITDQDRPIENTLSPNFFRMYELDAELPTDWKMKLHIKSKGMFDSVIGSIQIDIEDRVVGEEKLKQRIAYTVFKERFETEKEALKYDDSQNAERRKGYLSGQITDLQQRIENFDKKFKVPVEYKDLKIDGRQQASQGTVEMFLEVLPIDIAREIEPAPLQAPLPQEYEVRLIIWETFEIPKVATKKVVDIMVTVAMDASATGKDAEVTKETDVHNGSENGDGSFSFRMRFPLVIPCPFPRLKMTVYNFSPFGSNESLGEATMSLKPIIKRLSRDGKYEMPPTKIKLSHPNYPGQNRGQVLIQMKILTKQQADGQPVGDGQDEPNEDPYLVKPTEGRGLADFFKGAGFGMGNFMLYVKIFAGVFITVVVVMILFIKPGILVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WLJ3</accession>
    <name>A0A8S1WLJ3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tubulin-tyrosine ligase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0940101</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WLJ3"/>
    <dbReference type="OrthoDB" id="290395at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_125, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069">
        <property type="entry name" value="TUBULIN TYROSINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="759"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="759"/>
            <end position="775"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8189587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="782" mass="92557" checksum="3391EF7B56F46AB9" modified="2022-10-12" version="1">MQKDETYIKTPPIRKLNNGLAIKQKINELNKKSSIRFSDLTKEQASNQKISYRRQSQPRKPVHRIQQAITIQRNLSQEPQTQGTYSVVFPNNSPQKKDKRKVQTTNCDQLEEQKNSQVRIVKGFYPSDINNTKQILTNILQGMQQRVQAEKSFGIMGLQVVPFNKIMEQNTVLTQQTIRQKAFSHDKEVDKSQSFTEEIEKKIKCKIPQKCKQKQSINYNNASPVPPKIIAPQSSSTQIQNNNFKFSLTDYSSLSREQLFQTKLQNYQHFLFLVSTQSGFYTVPQDSLIDLKFKIGRGNNSLLIKEIFKLRWWWNCYKYTELIDSESNFIWTQIKVQNYMDTQNQSKNDIKQLLRQKINQPQSLIQKAKKIQIDYLLRIMNDDSDYKQIERNQKAYLKLINQKGYTLLKVHPNLKIHNHIEKNYHLGNKKALYHNLKRYYELTKQDIHSIIPMTFHVQKGSRDKVYLQFLEHYKKLSKGSTWIVKPGEFTNRGTGIIVCQNLTEINKIISKKQVHQNGKPFTYLIQRYIEKPFLYNKRKFDIRCYFLITQLNNIFRAYWYEDGYIRTSSEEFDLDDPANLYVHLTNDAIQKYADTYGKYENGNKLSLSEFQRYLENQPKKYNFYKDLYPQLIEIATISIKSVYCKLAPHKKEFNFEIFGLDFMIDSQFKPYLIEINTNPCLETSSPVLQRIIPQMVENAMRLSIDTIIPPPDANVWPPCKKHLIFYDNLLENNKFQLIFDEREDSEELIKLYGGLLEHDEIDEMDEEEEEYQSDDESQKFEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WMR4</accession>
    <name>A0A8S1WMR4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0980184</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000098">
        <property type="protein sequence ID" value="CAD8191304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WMR4"/>
    <dbReference type="OrthoDB" id="295687at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8191304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="187" mass="22222" checksum="17E6F358AA005FC6" modified="2022-10-12" version="1">MIQKENQTFKYPSINYFNPNSSFRRYVKDETQIQCIIELKMEDYQRSSISTEGSFDYSQSLKINKKSKIHKQKDKQLPQKSETKNIPKNFGVLLKKYLCSKNVDNQAIKAFLKNGENKKNFSRQDFTRLFNDPLAADLSREYFSGFQIIHDLMISEKIQDVKNHLKYISKFYNSTYNKQELDELKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WQD0</accession>
    <name>A0A8S1WQD0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPENT_87.1.T0960088</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000096">
        <property type="protein sequence ID" value="CAD8190379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="431720at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008331">
        <property type="term" value="F:high voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000282">
        <property type="entry name" value="Calcium channel subunit Cch1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000082">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000087">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000093">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000117">
        <property type="entry name" value="Voltage-gated Ca2+ channel, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050599">
        <property type="entry name" value="VDCC_alpha-1_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628:SF7">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="3" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="266"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="316"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="341"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="404"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="503"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="908"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="934"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="960"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="991"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1020"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1059"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1128"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1238"/>
            <end position="1261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1273"/>
            <end position="1296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1305"/>
            <end position="1324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1330"/>
            <end position="1351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1363"/>
            <end position="1385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1462"/>
            <end position="1489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1544"/>
            <end position="1564"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1570"/>
            <end position="1592"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1612"/>
            <end position="1631"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1681"/>
            <end position="1699"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1719"/>
            <end position="1741"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1761"/>
            <end position="1779"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="15">
        <location>
            <begin position="285"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="15">
        <location>
            <begin position="941"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="15">
        <location>
            <begin position="1237"/>
            <end position="1497"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="15">
        <location>
            <begin position="1552"/>
            <end position="1793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="172"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="186"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8190379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2147" mass="255408" checksum="03C9122EEC34D6D5" modified="2022-10-12" version="1">MQQVNAVKQQKQVSVFQQPQKGKQTQKKNKQDTQGDIKQEQFDQNKSKQQQASIVLDKNKPESQFSKGTQIMKESLKSSQKSQTLRSDKKRFTQNRTSQNNQTLILLQENQINEKPARDEESVSEQYNTYNVEQEHSMIILNEALTRQASKKAFEQLLKENESYRLDASNFGDFVDEGQHSNKQQQQREEQEIQDEHKKNNQIIITEESDYQEEDQEDIEMLYENLAYEESQNYNIELKVNHNFKWLLMEIKYYQDNNKLLNIRKILFLLIVGFEICCYHLLQWTAFDLYEMFLTLTNIILYLNRQSNKQIQQYQYVLCALYFIDSLIHLAGQGFKKYISSFWNIYDFLILILYFLFLIENQYIPFDVSTFRLIRIPFYVSKISNTLNIMLMSIQEAVKQIVENILILILFTILIALIMLYAFNGVIRYRCMNEELGIFSLEKFEEDVCGYVECEHGYVCARQLSNLDTPTHFDDIIFSYFQTIRTSVVNDWTISMYVLMKQFNPLVCILYIFIIFAINKFYFQLLIAVLKVSYHKTQEYYVKNPIKPEFYEDQISVNLYKLKNIGMWQYYQTSYKNYLKGKCGRLNSKSFSNNSQGEPNTKGQKYIFPEIVNQEQTKLLSARQKNEMINERQLIQKQYTFSSWIIFILFPDHKLQNQMKNLIQANELYFNEPQLFYTFLSIDYLSLNKQIIKGSPNQFNSIQDLQISIVQSKLPTHNKIKRSSKIHIHQNYVMDTKQQQTKDNSKKEKPIFPIRKSNFQKNYGQIVQINEENNISVRDLSDDEMNSNFDHSRFEKQYQQDIPQNKNQKNLQLFYTQRFQQSTQVSKSIPQLSKFILIDGIQLPYQQIEKIINAEMKTEIQEIKNYEEKYQIKISQISEDFYIQKWSGNDLIKNYKADSVINFINRGWNYYFAKGLIFYFEYIKFWLGIFITQQIVIVIIDSFVCIDTLLIAFSDYYDSSISDFFDLSVNILLVLEMLIKSICIKGYLCKISNILGCLVITISAIERILTLTIYQDEEVLIESFTYIRILKSLFFFRVIKYNTFAQNMMMISYQTFPNYAVMTLLLFFLILNYAVFSLQIFNFPNSGELALFHYFGNIYQSWIAVYDISTSDDWYGVVILSTTYSASYIAFLFCISLVFIVNYFGFGLAFVIILDGFASYLENVEEIEEEKIDEQDNNQTHNSIKIKKDFTLNNILSSLIQPEENEKFKNIKNSYSLYLISKKNRFRKLCFTITENQVFQLLMIIILWGSLINLIVITYTDQYRHEQWNICEIIKLICNLIILIDGILQIITYGLLSEDGYFKSIWVFIDFVYVICYFIHLTTYHEALEWILFLGYLRPLKVTSTYGFFINERGAIMKSLGDIISIFYVVLLFWFIFAIFGMTIYRGKMGYCGEATNYGISKEECEEEKEEWIVYQYNFDNILEAMQSLYVLSTFDLWAQTLFICINSAPETTGPIRFNNEWISYLFFFSFTFVGTLFFLQFFAGVIFVNFQQNKSQLLNPDLTLDQELFMKLSDIIMQDTPNYSKPPKKGLRLRAKKLLDNRYYIFITKYSLLLNLLILMLLYESASNHYLNILNGFHHFFSFILIIDCIVKLFSYHIKRYFDDIWRQMQLIFVICAIIDFWLDIEIGLFIRYVRASRDDPYFVWLRLFILHRCLRMTLNIQQFTRIRKLLNVIYFNKMALLRILGLFVTVLSCYSYIGCELFQNIETGFMMNDQQNFTNFFFGLIVTFKCTIENGWRFIYQDTLQHMKEHHEPWIIAQIYYYSLIFVGARVLLNLIVCELVQEFEKFYDTSSSCVETYVETIDTFRNLWCKYSEEFQGTKIQTKYLAHFLLELGEPLGAQKGDNIWDAAKHASEFELKKDFDGCLTFRNLLYEVFKATFKKSIFKISTKAGKQIMKNNDKKIKYRLYNQRNPHYRREVCSQIVDLQSNFNILQEYLYLYMAIKAWKAFSEHFFKAAEQIRNTIEYTESDVNIEIQKFSKIFCQGQDGDDIYNEQDDNDNEQVLQFNPVIRQKKRRPSAEHLRRPTDVYILPYYDEQRSIDKRAFDIDVKNDNSKYLLKPTDQKQQNKLKSISVIKKKEQLIKYNEYNKRRDAIDKQIQSRNKYLYGYHDKMILRQPVDDKQVMSRLDPMKVPSNYNIMKIGSRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WTT0</accession>
    <name>A0A8S1WTT0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1000023</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000100">
        <property type="protein sequence ID" value="CAD8191815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WTT0"/>
    <dbReference type="OrthoDB" id="447251at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="243"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="301"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="333"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="7">
        <location>
            <begin position="438"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="750"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="754"/>
            <end position="773"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8191815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="919" mass="108858" checksum="900372536A8BAD71" modified="2022-10-12" version="1">MQIKQNVIQSLINQNSAIENLVQKEGLQLIGDQSYGQLNDSEYNKKKTQIILLKDQQYIDAEQLKRLEEHNEDQLNQQQKWIILLLFKIIRPEEPKKIIWDLIGMSFIFIQMITIPLILTFSFEISGGFAIFNDIMDYYFLIDILLQFQTGYYEGGYFVSQRRQIALNYLKLWFWLDLISSFPYDDMLSLFIDESNSQSLKRNTQIIKIMRIVRFIKVIRLMRALKLKKIINQIEDTLFQDKTIISIISFFKICLIILSLSHWLACIWNAIRFIEETDNNWYTQYVINFDQEKDNDPDFWFNQYVAGIYFSITTMITIGYGDISPKNTIERSFGIFVMILASGVFGYVMNSIVLLFQNMNESLEDLLNKNTAAIKYMKQKEVNKKLQSRIKNYLEWLIEDEQLQKSYAHDTLEKLSLPLKNQLTQIVHGKMMNQIKFLSKNFSSLLLKNLAFTFQEEIYNPEDWIINQNDPINDQTSIYFILNGRVSVCFPKSKGVIELIELSKKQYFGEISFFGNIPRCASVKARNFINLFRLSRKSFLEQCEIEDIEKFFQLKFKIEFEQDYEDLNLFCYVCQAANHTAKNCPNVHYVINKYDRITIIEKYNEEIKLFINERRRQRKKCNTLKYLNNKRNQQVIQQITEEAYHIEKYINFHQTKPSVLRIKDSFRTDSIKEYTNFTPNSNLNSIARLQNYNAELYAENVRQQKELMKKNKLKSKFVQLFQNKKEQRQSLSNQSGNKFSKLVMNLTKLKKQQDQQQQQQNQEQENSQISISRLSSPSTIKPKLLRAPTMVARKLNEGKNTRLQRLPRKTYLGQICGFEKRNQQFIDVIMEDSMECSAQPLKQSSVIKIKKKKTIILQKSTESTIIESNQFPDVYFHKQLKRSQSSDQLKSNSDLEKYSKLFLEDMIYLVVNYKLQNCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WU65</accession>
    <name>A0A8S1WU65_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S ribosomal protein L31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1000096</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000100">
        <property type="protein sequence ID" value="CAD8191945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WU65"/>
    <dbReference type="OrthoDB" id="10253501at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00463">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.440.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000054">
        <property type="entry name" value="Ribosomal_eL31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020052">
        <property type="entry name" value="Ribosomal_eL31_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002258">
        <property type="entry name" value="PRK01192.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10956">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10956:SF0">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01198">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01380">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01144">
        <property type="entry name" value="RIBOSOMAL_L31E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8191945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="13990" checksum="31EB9535D7618BA8" modified="2022-10-12" version="1">MGKKEKGKPNPLGEVSRDYTINLHKAVHKETFKRKAPRAVSHIIRFAQKNMLTDDVRIDPQLNEAVWARGIRNLPRRIRVRFQRKKKEEDDGKGKYYTLAQYVPVDSFDNLKTEITKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X1E6</accession>
    <name>A0A8S1X1E6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1110057</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000111">
        <property type="protein sequence ID" value="CAD8195974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X1E6"/>
    <dbReference type="OrthoDB" id="282679at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8195974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="232" mass="27626" checksum="F644281B570105D4" modified="2022-10-12" version="1">MVYKIRNKSFFWTRAGWKNNWHPKNFNAPRPSSSEFTIGIRCRYDHNSFLRAYHSYRKISRHCKQYFFGNRELEELFQMGLRTFFIVPHIAECQVTQIKHGGERRMVDQIDRDFELVSYNSHPYQLFTYSVWNQYLANQQEAYEQRKNGGKAIEDQVIDHISELVKDEKSKLGPGKQLSIERTAEIVMNVMRQLRAAQQRPNLNNRRADGEFDDFLEQRRPFTAPNNQSATH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X3B2</accession>
    <name>A0A8S1X3B2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1100094</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000110">
        <property type="protein sequence ID" value="CAD8195712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X3B2"/>
    <dbReference type="OrthoDB" id="287263at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8195712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="82" mass="9526" checksum="D0DA9BD1C2895384" modified="2022-10-12" version="1">MSYQYREQESQNTLSQNSNNLQDTDLEDRIHCLPENEFCCKKMVNHTFVIGISKPGEQRIGLQTIQKNKITIKGRLGIDEKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X5L8</accession>
    <name>A0A8S1X5L8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">H-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1120157</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000112">
        <property type="protein sequence ID" value="CAD8196487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X5L8"/>
    <dbReference type="OrthoDB" id="304609at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019019">
        <property type="entry name" value="H-type_lectin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09458">
        <property type="entry name" value="H_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035721167" description="H-type lectin domain-containing protein" evidence="1">
        <location>
            <begin position="16"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="H-type lectin" evidence="2">
        <location>
            <begin position="50"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8196487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="427" mass="49159" checksum="1AA6D79A02D599CA" modified="2022-10-12" version="1">MLIIQFVLCIFSADADHLFEQGVIQHFNYVSNPNLILHFSSAKFRQEEVFIPFSKQFSQAPDVYLNVKQIDLAYTFPQGYTLDITSISTLGFKVKMVCESPELFFGVIFNWFAFNDDSVQVISNLNITYPNSQYTHSYMKNCKIDVALSNLVSFYAEGPQFNNVTVYNQIIQGLSLTTDTVTISFYTYNVKQIGYQILLTSSDLFLIGPTITNISPYGSSQIVNFPQGWETQNCFSNLLGFKHDGTDHNIRLDQRILYSPNIIVGFYPWHETIIQSIQHNYFCINDAYFDLAIFDGLMQTQFFDKTNQIETHIEILEINYNQNQNVGEEITIPQCIQSLKIIYYWKCIGYEQLKLQLFCTEVWCSSSKVQCNVDKTNIVRLSASFLFITASEQYIQITKSSASLTATQIIKQQNPFQKVLFKVEMVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X5N1</accession>
    <name>A0A8S1X5N1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1130003</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000113">
        <property type="protein sequence ID" value="CAD8196507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X5N1"/>
    <dbReference type="OrthoDB" id="287158at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="402"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="402"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8196507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="438" mass="50835" checksum="0609E3BE7EF0B739" modified="2022-10-12" version="1">MEICFDTIQDQLAKHSDLAKIVDFNALSQKPGFKIEQCNNRIYIGQMMNGRKHGIGILKVVDSLKLYIGEWVADQKQGLGYEKFSNGAEYYGSYENNKQNGYGEYFWVNGEIYKGHWIDGKKSGYGEWLSQNSWYKGEWKNGFVEGRGVYQSEKGDIYTGDFITSMKHGMGEERFSNGDLFRGNFKNGKPDGYGEYFWKCGSFYQGFFLNGMRHGQGLWQSSTSSLCDKYDGAYQFDKKSGYGEFTWVNGTTYKGQFQDDVRQGYGEMYSKGILIYQGDWERDIKIEKLGVRLKFQQKQQTQNRIAQQSKHLNVVEIKELEEEFNGSDPESLLQEDKKIKIKKMEKFRLTTLNVDSRTKTHRISPMKRSLDLQKCQASTQNKKNRAKSQTLCKIQVIQQNKQRSTSFQNEVQKTNKQTTTHIKNSQPKQSRIQLEKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X6E0</accession>
    <name>A0A8S1X6E0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1130150</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000113">
        <property type="protein sequence ID" value="CAD8196801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X6E0"/>
    <dbReference type="OrthoDB" id="308612at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="313"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="133"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="313"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="325"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="342"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8196801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="442" mass="51057" checksum="A779759D3648DA83" modified="2022-10-12" version="1">MSEKGMKVDPIILIQKIIRENTYLTEIDNKLDVDDFHNPLNVEETQSQLVDSIFGSCNVKSQKASKMISSSGNAKPTSNQPYGSLFRSGVSNYTVSDEDEIQNNSNEIKDGKQCNSKIDINDINYLKPPDLKHQKYQISYEELNQRCQQLELNLEVERKKNQELNDIILCQNERIQELTEIEKAAYEQMDSRLKEISELQAKNIYLEAQLQEFKDSQEKELKTRIKGYMDSNNSSQMNAMLQIKEKQIQLLQGQLSSLRAATQYRSQGSYHSTQSHDNIQDNKVVNQIINNTNNNQIIIKNEIFQKEMTSLIQKPKTSTPKKQQIINQDSQSTQRSKKGLNHNKQNSKDSITQPPTSWLDHIQKKSDDKLKILNNQPSEKELQQATLNNSINDNVNKYLHADLKITKVNSPAFQRKTTYQLALDNDNQDNKDFIKQKSFQHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X9I5</accession>
    <name>A0A8S1X9I5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1160067</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000116">
        <property type="protein sequence ID" value="CAD8197696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X9I5"/>
    <dbReference type="OrthoDB" id="512616at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13771">
        <property type="entry name" value="zf-HC5HC2H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8197696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="527" mass="62114" checksum="267770C1A02D9DFF" modified="2022-10-12" version="1">MNTKIETIPVLQEKCKFHQNEQANLICLTASCLNQGFFCFQCKNHHEGHNVIQGIEFFDSLKQLVSFDGSQKSSNNVNQVSARIVTAMLTAIDELEALNQYFSEILGSIRLQFEKYCQISNDVNVINNFLIFSQKESVEPQQFKDKFEECLKRLCLKLQKLHIKSTVTCQSNSLFVPDERLFQEYLEPLISTLKGIKLQAESSFTKPLYYNKLDLTFKNGLMPQVNSFFEPVIFETDQPKEEDKVLERLWIDKNETMRKMLMMKQRTKEEQVIDDEPFSYGNGRLEKVFAVAGSKVWFLDFIYSKFKPTSKHSKIEPKQACVICKCHQKFARINEFLNCKYGDLYGPYHLEARLLDSFKMAFDKRTNKDYLIDRGGLYFHDLCVYWSSLVECDEKKGTIDFESLQEAVKISQETYCYLCKRKGPTLKCNNENCQIWIHYYCWKELEPSQQYLDNQKFRMLCYQHVPAKYRKPAWASQNLLLDDHQLEKKESKEFRQSQFVKFTYQESGQSRYIQKLEQQATKQQSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XA77</accession>
    <name>A0A8S1XA77_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1170037</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000117">
        <property type="protein sequence ID" value="CAD8197987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XA77"/>
    <dbReference type="OrthoDB" id="286892at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035936772" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="432"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8197987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="480" mass="56100" checksum="042B8C26603CD1F6" modified="2022-10-12" version="1">MFFIQFLLLNLINTEQIQKQEIKRYSIIHGRVSTIQIPIQDVFNVEIHHLNKTLNKTKEGEEPSVLFADFCQPKNPSIYLDHIQSINVCADQCKIIIQNNKIYSLLPGYLKVLEMNDEQGFTFFSETLLQISQNLPEILITINIQQDNKLIILDGVSLFIINIDENDEINKPIKLHTSIEVVTQIIYYDNLIYVFGNNTLEIYIIQEKLVELISSINITKFEGQNQNIIGVKLKSPNHFIWITESSLGYVQLDAQFQPIQFNTFMRLEGRIIDVEIFEDLTYVIQKLNNKINLQYVLEYEGGQLKQNLSLTTKVRQIFSTQNYIIILQENLIQLAFAKIETQQLFSKNILENTNKILKYDQGQLIVQQQNEIVQFEIKQQQAQLVCEPNQNYSTGTYMMELRTGYFVNETKAYHNQLVEINVYFEIFENGNVGLYVGLTIGLAMTFMTMIIFCVFFYKLKAKYQLVREQYHKNSTEFTIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XCL6</accession>
    <name>A0A8S1XCL6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T1190052</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000119">
        <property type="protein sequence ID" value="CAD8198542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XCL6"/>
    <dbReference type="OrthoDB" id="123929at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00106">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000226">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF15">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="9">
        <location>
            <begin position="15"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="436"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="93"/>
            <end position="100"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8198542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="601" mass="69540" checksum="6ECA6E01839AAB9C" modified="2022-10-12" version="1">MQQSKLNKSTLQQQSIKVYVRVRPLLKKELGQECIIKTDQQKYVVIEQSTHILKQEYDQVFDEKVSQAEIFKFISPTIHKALQGYNTTIMAYGQTGSGKTHTMFGSDWEYSLNISLGHQSTFIDDLQTDQNHAGMIPRTIYTLFNNLQQGYYVYCSFLQIYNEKIYDLLQDHKVPQALQIHESKTEGIYVEQLTEYAVNNLYDCLTLMKRGEKNRMIRQTTMNLKSSRSHTIFQLLIETSKADSGGQLLRSKINLCDLAGSEKINKQDSMTNAHLNELKSINQSLTTLGKVIQNLSQNNKNINKLPIPYRESKITRLLQDSLGGNTLTNIFVNVAPNIYNIDETANSLKFAQRARNITQIVQANSINATDQELVNKLLKEIDYLKQLLNMKRKGLNSNDIHFKLMKIQEENERLRQANLSVNEVEKLMQENRKMREELQKLQSQSHENSESSHLMKHGSEEGFSGSEVDKQYNTYQQTKLSNNGLLIVSNPSMQKTEVTVRYKDRGNKMQTKLGYVEASDQMLQREKRLMSERKRVIERLELLEQLQFKNNLNSSLPLNGKIPKPSKKYSYNLLNRTVEEERISLPKLKIAERKLIHDQLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XF01</accession>
    <name>A0A8S1XF01_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2HC/C3H-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1210161</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000121">
        <property type="protein sequence ID" value="CAD8199401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XF01"/>
    <dbReference type="OrthoDB" id="300917at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026319">
        <property type="entry name" value="ZC2HC1A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049899">
        <property type="entry name" value="Znf_C2HC_C3H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13555">
        <property type="entry name" value="C2H2 ZINC FINGER CGI-62-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13555:SF36">
        <property type="entry name" value="ZINC FINGER C2HC DOMAIN-CONTAINING PROTEIN 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13913">
        <property type="entry name" value="zf-C2HC_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52027">
        <property type="entry name" value="ZF_C2HC_C3H"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2HC/C3H-type" evidence="8">
        <location>
            <begin position="713"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="domain" description="C2HC/C3H-type" evidence="8">
        <location>
            <begin position="831"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="131"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="513"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="631"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="90"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="529"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="569"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="631"/>
            <end position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS52027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8199401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="871" mass="101629" checksum="63BBF9FE130528CC" modified="2022-10-12" version="1">MQDLRCTIADHNKKKFVSITLQKEAEKRLFCFQCQSDAIGGDFTKSQLVSKLKLCDLDEVMEGNAYSVYNWPADDEGIKVKDFLVGYKSIEQYRKDIEDFFLQMKQKLNEQVENVKQLLVEALNATQFMKDEKLKQEEPEKKDAKKDDKKETKKDDKKETKKDDKKDTKKDDKKDEKKDLKKEDEKIDLTPTIENLKKHYDTIYDLTKIKTIIKENVKSDPNLLSAKINDYLNDQKRSEQIINMRKVFQGVMSGKVGINQITLHKQVFESYWEQIQNSIQQWPYKFGKPQWKLTPSKADESKLIKQLADDFKECAGAGLQSQIIYSDISFSEGQHGCIVLFKEFDQKRAGANGNFYVGVVPEDSKDKSPYGHGYRKDLFSQNGEGLNVRIGTDFNNVFKMPPKLLAVKLDIDNNYFEICDGKRTVVYGLEKDSKLKDQKWRIYFWWNIAADRIQLIHILNLIKPSKPTQNMSKQFKQNPQNSFNKLFNVQISVTSRLKIYDVSRTSKPLQNLSNIPIRTQEDQIKKTQTKSPLRQNFQKNPLENKSSIQNLDEKSINKGKSPLSRNIKTSEQQQQLQSDKQSTQQKQTIQFKNNEQDQLNTNQQNVLKSQQNIKQQDVLIQQKQQINLQKQSVQQKSQNNQQQLTRPKTTTKSQLQLKQIIQQDQQINSTQQDQLLKMTHKTKSQMNLNQKQEQPIKNRQQQIFQRKQSIEDKLLQCPEGCGRSFNENALEKHSKICKKVFQQKRKVFNSQMQRMIEVENGGQDRGCQMKKQVHIQANKLGQKQQQLVKNEKPKWQAQSEAFRAIIRQAKGQQLTQEEQVQLNSAMESVQDLVQCKFCNRKFNSEVAKKHIVFCETKTKQCGKSGMQKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XLP5</accession>
    <name>A0A8S1XLP5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1290041</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000129">
        <property type="protein sequence ID" value="CAD8201819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XLP5"/>
    <dbReference type="OrthoDB" id="297651at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF1">
        <property type="entry name" value="RAS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8201819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="631" mass="74510" checksum="2B337E1901C41D93" modified="2022-10-12" version="1">MDQSNTKEVHLNSKGLDQFPIILQVRYLNLSNNNIKMIPNKIVELQQLEILILNHNYISAFPKVQMPRLHTLEIANNELKTTDFSNLSLVCLDIQQNQFEIYQHLSSIQQLNMSWFLLIDMDSKQQNLLRNTIGQLLRNHREFNFTHFVNVFSSFTNTVLIKAIQYDERSVIPSICIYHPEYLMRMYHPQPIILAGKLNIKIFKILLDHIDPCSKLIRFVFLWACSKLEVEMVEYMIEKQIFGVWEEKMLVISQNVGTPMKGVNGDTPLHFVLRKHCRNQNEYENQSKLIQILMRYQNPNQKNSQKVAPIHEILQVPSIRSFQSCSEEYFDYNKKMGRQKDSLMHQAASLGYVQALQYLSGKVNIFSVNRQNKTPKQKCSPSVLILKYARKLELQDLIANLILVQQHLSVSVRNFHADFHIQINGEDDLYQLFFKDMILQDKLIVVILLNMLQFKQLYNLKFTSQRVIKPIQYINNCQHNFKPDIKQFKSLLSDQLSANKRMIQNPIKQQEVIIKNSIKSSSICLDQIEDTSHDLYDIMILYQMIGINGDLSNRTVLLDYEDFQQKQFRKTCFQQKINKNYFTGRQQSPLPVRYNYDNYITYRKIKSNNNYVKEVREYSHSANQIERRRIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XM09</accession>
    <name>A0A8S1XM09_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Homospermidine synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1280140</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000128">
        <property type="protein sequence ID" value="CAD8201688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XM09"/>
    <dbReference type="OrthoDB" id="2151234at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032095">
        <property type="entry name" value="Sacchrp_dh-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16653">
        <property type="entry name" value="Sacchrp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Saccharopine dehydrogenase NADP binding" evidence="1">
        <location>
            <begin position="21"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Saccharopine dehydrogenase-like C-terminal" evidence="2">
        <location>
            <begin position="165"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8201688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="482" mass="54833" checksum="91409DD86C145688" modified="2022-10-12" version="1">MQLINRDFYQFSKKVNLPKTIGVLGYGAIGKAFTEILLKQHPQANIVILDKHDAFFPKEKRFKTIIYKQTRDNLTQTLDVMGLQQGDTLVDLSTNIGFQEIWTLCAQKGVQYLNTALEVWEDSEDANSCPQNADEAYKLTLGYIKDNAKKSPFWLKGPTALLETGFNPGIVSHCVKRGLEDCAKHYIENSLRDYNLNDLRRYLNQKNHSKLAQALGVHTIHCSETDNQLMSEIPKDVKTKFYNTWSCRGFLTEGLVPIQVARGSHEDTHIPNCYTIRDGKTIVSKIPSVNIWAKSWVPNEDITGVLIPHGEAYSIQDYLSDPETGYSPSQYYVYDYNPLAKEFIRNLPLDATIENTHPDMEVIHPMNYPSLKGWDKVGALLIMKNNRAWWTGSIMDEIDSSKIYNGMFGPTVLQVVAGVYGGFLWTCQHQNIGAHFSEDVDTDDLIRISEHLMGRFISIPVDLTKTKIKDCYKLQSFLCQQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XNM9</accession>
    <name>A0A8S1XNM9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Calcium-dependent protein kinase 1</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="ORF">PPENT_87.1.T1320165</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cell membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Parasitophorous vacuole membrane</location>
            <topology evidence="24">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="29">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDO010000132">
        <property type="protein sequence ID" value="CAD8203016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XNM9"/>
    <dbReference type="OrthoDB" id="286046at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020002">
        <property type="term" value="C:host cell plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020005">
        <property type="term" value="C:symbiont-containing vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000398">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000199">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000938">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 26" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17" id="KW-0969">Cilium</keyword>
    <keyword evidence="15" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1032">Host cell membrane</keyword>
    <keyword evidence="16" id="KW-1043">Host membrane</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0519">Myristate</keyword>
    <keyword evidence="12 26" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0564">Palmitate</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="27">
        <location>
            <begin position="106"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="404"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="443"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="479"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="28">
        <location>
            <begin position="515"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00060437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00068067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="EMBL" id="CAD8203016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="553" mass="63705" checksum="4BF1675C4F09B56E" modified="2022-10-12" version="1">MGCCKSFPNGCCTSKSLENQTNSVIPKENSEQFQNEFIQSILYLDNNCNVHKEKEICFPIKNSLYTKDITDVEIKQEYMKTSSKQWNIKQTMFVRVNSNKNINDSYLIKEMIGQGGFGKVYKVVHKQTGMIRAVKMILKEKMKQEDQERLLEETAILMDIDHPNIVKLYEIFSDTYSYYLVSEYCEGGELFEKIKLVSILTEKEIANFIKQILSAVSYCHQKGIVHRDLKPENILFDQKHDQASIKIIDFGASAKLQNCEKLQKRIGTPFYVAPEVLDANYDEKCDIWSLGVILYILLSGYPPFMGCNEQEVLIKVKKGEYTFDPNDWGKVSNSGKDLIRRMLLYNPINRISAADALNHEWIKNNKAKGQINSLTLSKLQDFDSKNKLKYAIFQFITVQVVTNQEKNDLLKIFQEIDKNGDGTVSKDELYQAYLKIHKGDKLAAETVVEELFPQLDANGSGIVDFSEFITATINKEKSLSRQRIEQSFKLFDLDGNGLITKQELNELFDEEIDEEMWQEILDQCDTDNDGMINLNEFINLLENKISKNPLFKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y300</accession>
    <name>A0A8S1Y300_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1500116</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IFT57 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000150">
        <property type="protein sequence ID" value="CAD8208386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y300"/>
    <dbReference type="OrthoDB" id="423881at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030992">
        <property type="term" value="C:intraciliary transport particle B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905515">
        <property type="term" value="P:non-motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019530">
        <property type="entry name" value="Intra-flagellar_transport_57"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16011">
        <property type="entry name" value="IFT57/HIPPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16011:SF0">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 57 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10498">
        <property type="entry name" value="IFT57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="141"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="270"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="164"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8208386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="394" mass="46030" checksum="610036FAAB0CEECA" modified="2022-10-12" version="1">MSEQQAGQEQLDVTAMMDELYDKLILLNYEQTYLKQKGGKPLNRAYFVNQTNSSEQFTQFKTLVKWLFQQNDVQTSDFNKLDDPVTLSQNIINELKNIGIEVDFPPLKLKQGFGEYVVYVLLQLATKALQKKKFQYKKAKIEQQSQTRQDEEPVQETGSVSSDSDPEVASDEEPEDVFTEQGFQKDEDKMVIESNVNPKEWAKEVERAAQKIKIVIKPDAGEWRQHFDATKQYSNQIKTILPEARIKLERMSDELGEILDRITKREYNINENMSDMCNEFKKKNEEYKNIELQYQNYTNAKKEMNDQYKQIQEKFETVQNKLNEHGSVSTDQSPVIRIKASLTKLRLEIKQMDLRIGVLSHTILQRTFHDAKAVQERDYHENGLILNESDELTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y419</accession>
    <name>A0A8S1Y419_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1490127</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000149">
        <property type="protein sequence ID" value="CAD8208083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y419"/>
    <dbReference type="OrthoDB" id="291219at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8208083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="431" mass="51589" checksum="FDFC453A8427969E" modified="2022-10-12" version="1">MKNKQYQIRVTLDSTPSTYQTNETLSLWENLQRSKDSPRKSKIRIVRDPLENECPLQQPNPFLFNEQLKHSFRDYLEGQKTALNLVNECNHIISQSHKMDQLKLFTNVNKFRSQLKQRSSVRLSMNQNNSQSEDLLQRKIKIQERFHKIKTLIQQRMQAKNKKESLRDVQLFKHLFETCEKNVNKIKSKNLLSEKADGIREYDKIKYSKNPQGSLRVRLDNDSNQNKCNNLRSKTLPDQQTIHLTRKKEREKMNLKLTEFLEHDQNQEKNLLLKAKRNLIRELKNGNQKFHQFEPNRYNITDPLSKDVKFYAYMFMKPKNDDILQKKNQKYKMLCKTERIQQNNQRHYLANFNKLQSNSRDESLEEDSSFNSMLEINIDNLYKRSTDLQNMILNTKSEKFVKRIRQFQKVQSLTTQIIKVNEQKLSNTKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y6A5</accession>
    <name>A0A8S1Y6A5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Golgin subfamily A member 7/ERF4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1510077</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000151">
        <property type="protein sequence ID" value="CAD8208588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y6A5"/>
    <dbReference type="OrthoDB" id="161885at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019383">
        <property type="entry name" value="Golgin_A_7/ERF4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10256">
        <property type="entry name" value="Erf4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Golgin subfamily A member 7/ERF4" evidence="4">
        <location>
            <begin position="58"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8208588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="160" mass="19274" checksum="70A38CC07B66E826" modified="2022-10-12" version="1">MSYESCDLQTDSRYPFDPRANAFELAKMETIRRKQHPLILITRVKSNGFHYFIGPSKSYSNEYPIELEPYISKEDFEAFINEVNNVIIKYWPCFFTFILAFGFSIVSCFLSLYLPRVCIEDVERFLGYVLERWNESWEEKMIKVEYKRNLFNSELQFYKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8T0</accession>
    <name>A0A8S1Y8T0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1570060</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000157">
        <property type="protein sequence ID" value="CAD8210001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y8T0"/>
    <dbReference type="OrthoDB" id="10264376at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002098">
        <property type="entry name" value="MGC78960 protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050630">
        <property type="entry name" value="WD_repeat_EMAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720:SF53">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 4 WD40 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720">
        <property type="entry name" value="WD-40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="182"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="502"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="668"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8210001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="703" mass="78761" checksum="1E3B2AF1D02FCCD9" modified="2022-10-12" version="1">MNLRPQPGHQNKNGINPITNTPIDYAQRGNQQPYYKQPYPQPPIQYTQNPQQYQNPYYNQTLPQVVQQPMPEAGQKLPLQSDAIELDHMNGFSGRYRDVIHLHPQKESTIVYALAGLIVIENLNDKHQQLFLRGHDMDISALTLSKTGRYIASGQMGSRNAKTPEAPVILWDFNARKPMQIFRGLRDEITNLSFSSDDKFLAATASNNNLIIWNCQDYTIVHNKLLEVPINLITWCPPRRSSTKHSQYLIVTSQGSNIFLNTMDFDVASMQYQLKQAPCQLPSSGLVRNYTKAVIDANGDYLYVGTHAGEICIFSISSQQGGIFKATIPISNNGVLSINIFGKNLFVGSGDGKVKKLTGSDTRWNLERELCLEGRMNSITIDPNGNELLAGTSNGRIYRINTAHLDSSVHTEGHQSSIVGLSVPLNACDLFVSIDYEGVVMVWDMNEMLVITRCIPINMNRVKGSSVYLDPDRTIISGWRDGFIRAYMITNKPVSPIKWEIVNAHKGAVTSLYSDQNYYLSGGEDSIIRVWSKTARQLITQISIHSKEITKVFPDLMKPNFIHSCSADKTIYTYDLKTDKKVIQHQSRNGVLLDMTQRKDNELELITCGINMPIQFWDIDIVEPVQSIDVMKLNSIAISPDGKILATGSDSGELILISILTQSILGKYLGHSSGVSKVAWTSDQKQVITTSYDGTVCLWNIYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8X6</accession>
    <name>A0A8S1Y8X6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1570085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000157">
        <property type="protein sequence ID" value="CAD8210051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y8X6"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8210051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="196" mass="22533" checksum="89D1FC0C00CFE882" modified="2022-10-12" version="1">MRQSNNDKNLPPQGNSIDKQFYYNLLSLEKAIIKEPQLDAVQQALAMYRKCVEYFDNIQDPIKYYFLDKIQQALSETKTLMLIINSKSEIESSKTISPVKRSSISDLPPPLLSDQQIMDKKLRSKQVTLQIKLHEAADQQSEMLNTMVTDFTTNTTSTDQKVRQDLNQQADIIQARILKRQQSTKMKGCSSMQILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YD58</accession>
    <name>A0A8S1YD58_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1660032</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000166">
        <property type="protein sequence ID" value="CAD8211995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YD58"/>
    <dbReference type="OrthoDB" id="296075at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8211995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="227" mass="27510" checksum="613C4AB387A3F4BB" modified="2022-10-12" version="1">MFEQLSSTITNRPLTFAKFTFKKLELEQDEQLRPILNGIYQNQKQGSMLTTELWSKKQCRQYLKQTNMQTDKGILKRFAHWEWDQNQNKERFKNLIDKHKQTTYTNKSELVTKLQLGTIEGEKRLRQKIIRDWKIQKSYDIQRELVKYKDQEIYKQKGIKYHQSSQSFGQLSLRQQTKNNQLNHIITELSELQEDTKIEKKKFDQVTQQKRNTNRLQKISFEQFLQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YJ53</accession>
    <name>A0A8S1YJ53_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1640051</name>
        <name evidence="3" type="ORF">PPENT_87.1.T1640052</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000164">
        <property type="protein sequence ID" value="CAD8211582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000164">
        <property type="protein sequence ID" value="CAD8211584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YJ53"/>
    <dbReference type="OrthoDB" id="299788at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="18"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8211582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8211584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="254" mass="29725" checksum="7F8AFDE95D96BE94" modified="2022-10-12" version="1">MNIQDNLTILNHQLHISNNKLQLQVEVLSHQNNLLKKKIVEQQNISKQIQSLKNENNQLKQDILALQETLYNLQTQKNNEIEQSYSSMLLAYSKENEKELTYLKKEYKDHIFRLQQEKNQLFEQAVSMGIQNQTAKNLELQTTKIIQIAQKLEKILYESPQNQESKKEKVNSAREIYDNLINQLNTDLNKNQKEYEKNDKFYHTLAASKNSNSTKSKDKPINFTKLLSLTRTNESPRSQAYLNALSLQTGNKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-06-18" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YJX6</accession>
    <name>A0A8S1YJX6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chorein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1700041</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000170">
        <property type="protein sequence ID" value="CAD8212687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="299244at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8212687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2252" mass="267282" checksum="2D0185BE25467A7F" modified="2022-10-12" version="1">MQFLKSYLTDYINTYVYEYFDNISKEQITTQLTKGQIQLNNMQFKPSIFKKLNLPFIIKESMINNISITLGKQSSIKIDTLNIVIEILNLYGNFIMESAIKLQILEKFRNSLIENLLKGMAAQDKQQQDPKKKFAFNLFQNIEIELNSLNITFLDKYCTQNSFGFKLEHFTCKSSMNPNKPKLRNIEIKNLLLFWQDPITQLNAYATLEDVAEQESDNLSFQLGIIFDQLTFNCSKIQCNAIYKFSEYVSQFTIRQNKQVEKSSNNEDDQKVKGEIKNILKSISLLEQSTECQDYERLCLLVQSQSINDLKQIQFEIMKYDKGEEIVQLRKKKLGWFGGMVKNDKKMGEKIINILDDLDQDPNFNLKTLQLKFELVVNQCQIGLSSNYRGQLNKLIINFKNVNLQMNKTNQLQLDFTIKEGEVLLQYQDKNNYLVSKNKTFEEKYSLELHYTNDEKKESLFIQTCPFQIVFNEQAFEKYYDLFYFFVVSENQLPKLTKKAKQQIQDIVSDKKEKDIQIKLEKVYILSTNGKETFLFSPGSIDFNYKTQNQSIYNIKIFDTTLGFSEKINSCIDFIHQQYCFNSMIMSRDFIKITNCDIDLVIQVQDNQINTVLELGDMNFQINPTIYKYFYQFYGTHYQQPLKEMKNQLRNNEENQQKFCLFSNTYIKNQIKCRMTFQGNFINLYEVEKDDKIMKIDLQNIQFKQFKDNQHYILELKTNDQIIKLAYSNEEDIQLVVNKCQMSRNDDYYVQNILEQNLNNSITNIQNQIQQQEFLSYIQIKLNSIHVVVLGNQNQPFINIHLTYLNFTSLGNQLQSKLTISFKYFTIIKEFNQQMNNFEDKIFLDIQSDENTTLIEYCQTLNENSILKLYLNYCILNYKAKTIGLILEQYPIELKKDWNLTKFIINSYLTYYPDLQIICNQMSVEIRKKQILFKQSELKSIQFQQNYISIMFMQDDQDENCLQVNAIPKGILIVDNENEMTINIRLDEAQAIFAQQYTLQTSKLKRKNNKKVLLSSCNIDFGWRVLFQTNVIKHFMNFRTNQFKFTFGTKDICYLTKQMQKLSTQISEFYASHDLANYLIQYRVKTYQLTFGVESQMQTVFIDDYFNQSLPIFGLTFNQPIWKLELNLDHINFHTRNLFEAVNYHSTKLAWEPIFEPISLDFSLYIQNQQPRIILDFNISDIVNIDISTEFIQNLLKFKKLVKQFENTDLKQNNEPINQNNNQILTYETYEIQNACGLLIIAYGQDQEPIQLINQQRSITKCKGIFQFEIIGVYSSIKIENWSLQVQKQKIIIEKGFSLIIETIIDEFMNSSKTIITSSILLINLSPFNLQIKIQNQEIISLTKSEKQQQIQKFQKYTNIYTIPNQLQKGQFCLIENNLISESYNYADIAQKIKKGNSEIVNLNNKFFRLKSLQDPIMNDRSIISITPVLEFINQTPFIIRIDKHCPQIKQFLHFILNSNQIVEDCEINPQKEYLFSFKIERYYQSIQYSFTELMKGKKVVLEDEKWQLNVDRKYLSLILIIKPKIDNYGRHQITIQFNQSYLINNTDLDILIYQGTEKNLEILGGQKYNTSFQQIVLQNIQEKQFIVFEERNSNLKSQSLALSEIIKLETIQIKKKVNNLLYFLPIQIEKQFIQYGDYTIPFFQIYYKYILYNRLEQPIYLCYEDKTTILESLIKKPLQFWKQEHQNNPDFSYECSLKLIFNDQVLQTERFNIMIQQQFVLLIRSINLRIRKFIKITILEKNHEYFLIIDDIQDKQDYPFMIINESKSLQIQLKSDNEKLSSGDSFPLANSLQSDQDFTYPVNLKDQTMNWNNSIQFLLKLDQVGLSGIIEINGQKYDYGVVKVGNQKQFIFKDFQKQRPSQIVQQIQQEKILYHFDLKMQIIGCTISYIENLKTKCKELLICSLENIIMVYSSNEFNQKQINFKLQKLQIDAQSKKMSQQKTVLFSEVSQNLNSIEFELKLDAMTTESYYSLDLVKINLGNFQFCMDKILLDKYGELQAQFYDFFNSQHTQKQKGNYLNKLFNKKKVERALFKNQLKQTRIFVRKLILCPMKMKISYLKDNYQLEKQHFVKENFTSSPSNLFHRILKQYIFEDKKFFFNMVGQYLTNKLMINLVKLTNWNEIRIKRAIYEPTRVYKVFNQQQANHQYMLKQVQKCDCNRELSKIKFNIISSHDVLLKGQNKILFICDQQIFFIHQNTIQQFLEYTDIQLFYSQADILHLYIIIEKDQLALLFEDQNNLQTAIQNLSRFQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RXK1</accession>
    <name>A0A8S1RXK1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0010478</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RXK1"/>
    <dbReference type="OrthoDB" id="308200at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="147"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="46"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="73"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8132172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="818" mass="97449" checksum="62929BCBB1E6CD72" modified="2022-10-12" version="1">MQQGNHTPSPQRSNNGLQGQQGRSQNQKKEFKLSQKKMINIPIKTQRINKNTQQISSQRSPINMSTKTTKQTIETKRSVSPEEFKNKRRKSPQQTKQQSSKDKNNIKQNNSSPLKKYIKQKQKSMQIKENEIKLKLKDNEYKKQCNLDKLKKKTKKIIEKQKKQKNKCLKEKVQQKQKVIEIKKKFEEITKRFEKFQNTQIEQFQHQIDQKQQQNQQKEEDIDNLYEYYLNQAALTIQINWKEYLKRQKLKRKFIQSQKQSIDWEINAIMSQQTLNSDEGLLFCQGGEKQVHISNIRSSVVSEESLSIMNQLNQELDNWNIYIQSIVKNQDLSQINRQMQQQIINIVQNHLKKTESQSEKRSENEINQERSFNSYRKKLSDELSKSSILQLQQQALKEEGKLLGFREEAIYLRYETEKKSKYCDNKQSLDEWLNKELEDLQNTRKAIEICHKREISALKKIQRDILIASEELDQDSSENQQIQLISKQIDDQFNQVTKQKQENIQQEDIQKTINFLTGEIFNDIIDELIDEVQNNRENFYIFFSDLVLNKDIISIAPTSVKEIQSYLDALFKYILENYSIDLLKNLNLPFGFTPQKRMKLIHGYDDDENNEEIDQNIAYPLSDTIFIEFENQRLQEFDLNDQIAFAIRELEHIHNKAIFDACNEALNYERPYFLNSGIVYPWQKINQRIIMPNQLEQTLEQSKKKVLDWSSFLCGFLPLEENQNQNIKEEKQKIIEEKKLSMKQQQLNLFENSTGSEFNSQFDNLGLIRDEQLYKLLISDIKESENKWYLVEDDRVDYLMELSDQIFEYLIEEFQKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RY25</accession>
    <name>A0A8S1RY25_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutathione S-transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0010542</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10257659at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051369">
        <property type="entry name" value="GST_Theta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43917">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43917:SF8">
        <property type="entry name" value="GH16740P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="4">
        <location>
            <begin position="87"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8132300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="328" mass="38369" checksum="2918515E1FF4B382" modified="2022-10-12" version="1">MSLILYFSPSSPPSRAVRSLLLLSKVIFQGKIVDLMKVEHKTPEYQYINPNQYLPVLIDGSLKLFESHAILKYLASKKKLTQFYPKDIQNRARVDCYLDWSQTGLNVIETYTNQCYLFPMLMKKPIPQNKEQMYQDTIETLKFFESIFFQGRYIQNQPSITIADLKCIADLTQLLLTNLDFNQFPKIRNYLQEMFSIPEIREAYAEYLDXSMLWDQICKNGIDHVTEWFIRLLTTNDTVITYQSHQFIKLETVQILYELSNTKIMSNIDIQQFVDLLQQAGEEAGLMSLDQEELDEFVPLEICSEFIKNFLNGFKTLMLEIGFSNNGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RYF7</accession>
    <name>A0A8S1RYF7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0020208</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RYF7"/>
    <dbReference type="OrthoDB" id="293044at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8133056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="187" mass="22046" checksum="2937010E44C44CA0" modified="2022-10-12" version="1">MSHQTPNRFHLPSIVSKEVSPTKDTTTKRTLRPSLFLNQLDVLPYSLKTEGEITDRNNGNKMYTQFESDPLIEIPYHFHLPKIVTTRPKRFQRSLPEIPIIVQESRNATPDEFERHQKRTKLFLKRQDSKILAPVTKFQRKKVEFKKSLIVIDIETGNQDKQDISETQKPLRRIAHQKTKSFQIRNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RZU4</accession>
    <name>A0A8S1RZU4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peroxisome membrane anchor protein Pex14p N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0010399</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RZU4"/>
    <dbReference type="OrthoDB" id="307291at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006785">
        <property type="entry name" value="Pex14_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF1">
        <property type="entry name" value="RAS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04695">
        <property type="entry name" value="Pex14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Peroxisome membrane anchor protein Pex14p N-terminal" evidence="3">
        <location>
            <begin position="14"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8132014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="285" mass="33159" checksum="B60F885AC8EC63CC" modified="2022-10-12" version="1">MSKQTKQEKEKDEKDKRIAVAINFINTPNLQNVPRSELEKYLKKKGLSDPEIIEAFDKAKQQKEEIKAKEDEKEKDLVTKLQKYGGIQQQNQISEKFINPQELKEIVEQAKISNLISIRNRGVTTIYEQILGLNNLQILIISNNKIQIIPQSIKNLSNLKVFHASCCNLQDHNIENEFFELTSLQELNMAKNQLSNIDRFTTLSELVLLDLSNNEIFRIPQNIDKLNKLKLLSLRDNHLTYIPIQLQKLTALKKLYLDQNDLDDPHEVLKQNNPELIFQQGLFEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S052</accession>
    <name>A0A8S1S052_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0020497</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S052"/>
    <dbReference type="OrthoDB" id="297005at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8133616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="211" mass="24889" checksum="9341A08691897977" modified="2022-10-12" version="1">MGVCALTQKGSSRKISKPEVGLILEGVTKESQINESYKTLIGQTQTVFENLINNKIISRNISQTEYEFYSKQTEELSLLNTYLQNYYNNYYRIVVSNIKSENLYYFENLNLKFIIQLYLAMKTIKNNGKEWWNKQTFYSLEKLITNRFDIYSKVVEEGRITPFLNFILLLMKLSIKLMQMEGMTLQEKEIQPIFDTKGSMLYNQIKLELLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S1M9</accession>
    <name>A0A8S1S1M9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPENT_87.1.T0020427</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S1M9"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000315">
        <property type="entry name" value="Calcium-dependent protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="14"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8133482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="320" mass="36625" checksum="FB9D8423B7714A3A" modified="2022-10-12" version="1">MDSIYKTANIKDFYKLDKILGEGSYAIVRKAIRKSDNIEVAVKIIDKVSLESDDHLAIQSEVEIMSQIDHPNIVKVLEVFDDKQKLYIVLELMTGGELFDRIVEKELYNEKEAADVIRPVVDAIRYCHSMGVVHRDLKPENILYTTPDPDATVKISDFGVAKVISDELMLTACGTPGYVAPEILTGVGYDMAVDYWSIGVILYVLLCGYPPFYEESNEKLFEQIKSGKIDFSGEQWEKISKEAKDLIEKLLKVDPKQRYKADQICKHPWITGEKALTKDLSDVTEKLRELNARRKLRRAQLMVLATTKLQRRIQQHQQKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S6J9</accession>
    <name>A0A8S1S6J9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0030390</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S6J9"/>
    <dbReference type="OrthoDB" id="422206at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049680">
        <property type="entry name" value="FLVCR1-2_SLC49-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10924">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10924:SF6">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 49 MEMBER A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8134604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="197" mass="23476" checksum="4CC9026578E5CF34" modified="2022-10-12" version="1">MNKLYPTDNIHHQYVEYPQRWLQLILFLCALLSNIMFGFSLSPIVKEISLVYQVDSRYLQFLTISFTVFSVIMIIPGNILNEKYGVRMSIMIGCALTFIDSIFAIFINVSFWFFFIGQLISLIGFPFRLISASKFVANWFFPENRILIMESLHQFLMQVQEYLLEFHQLFQETFRYTIIQQMIRQNQDVLQLRVQCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S877</accession>
    <name>A0A8S1S877_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0040380</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S877"/>
    <dbReference type="OrthoDB" id="305875at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="71"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8135717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="201" mass="23270" checksum="1A7D85179BAAAD75" modified="2022-10-12" version="1">MNSNTKITLTIEQLTELLMNPQLPNIQSSLQSLSEFELRNNNVDLMKIVSFVDQDEFQAVSTSTSSKNSQTQQMIDKINELELENIFLKNQLEQQIEKENHLKQQMNQCLKESQKLKTDNERLNKQMDELEALINQNKVISNRLDPISKSVHLDMKKALIVKPLTITQPLRKFSGTSGIVTQHPFKNSKDLIRIKTERSYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SCA8</accession>
    <name>A0A8S1SCA8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0050316</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SCA8"/>
    <dbReference type="OrthoDB" id="285248at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8136742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="315" mass="36207" checksum="05AA16E65B6806F3" modified="2022-10-12" version="1">MRRPNEVEYNKTALMANWYEDRLAPQQLQRDPNAQKSLRTVEEGISIPGDNGCLLPLPRVNRNPPYITKEIIVPDDGYREFRTEFQTKFDAKNIQNMQLGDCRALVKTNGEKVNFPECNPTVAQTAGARSIKQLDQTNMQQILVDRAVHQNYTDFGSTFRNHPEQHNKFYSITTYQQSFQLPENNVKEILTDKNVKPIAAGCRERDPFDQGLKMTSVITGEKYRDSVDPKENTEVQRQWVHCGDSGLKTAQNNLRSSSIQNYKKTAYQVHPYDIATSLPLGDGVYTLHPKYTDPGQFRHIRSDVTRIMNKPITRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SEB9</accession>
    <name>A0A8S1SEB9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0060460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8138120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SEB9"/>
    <dbReference type="OrthoDB" id="301662at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035913392" evidence="1">
        <location>
            <begin position="17"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8138120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="350" mass="38237" checksum="419B00C48CDD50B2" modified="2022-10-12" version="1">MKVTITLLVVLALANAGMVQRFPLTYGGKRSIMNIMVEVENKIKSHSPLDTIRGVLDNFKSAVAQEQGTHDEVYGAQKAECESEIAYRRAEVEDATGTLKIANGILKTSNILLKKTQATLGETENILNTVSLHIGLINDARKEDTQSYNRGAVTFNDAINAIDDSLDLAVALAKGKASLIQVADMTTRLMKASVATKMNKAFMTPLAALAQITQEEDAAGAAERLVQLLQTLRANVEEAWASYTAENTNALALFTQQKDLYLASQTRLDASKGRLNTKAENLQGVISVQTAVAQAASNKKQRNQTLWDDAADLCHSFDVEYEAVTAGRRQELVLVQELERLAERRAAEQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SFR2</accession>
    <name>A0A8S1SFR2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0070264</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SFR2"/>
    <dbReference type="OrthoDB" id="433924at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00024">
        <property type="entry name" value="CD_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051219">
        <property type="entry name" value="Heterochromatin_chromo-domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22812">
        <property type="entry name" value="CHROMOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="4">
        <location>
            <begin position="3"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="77"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="89"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="107"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="117"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8138690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="297" mass="34560" checksum="2BA6B58D6959BEEB" modified="2022-10-12" version="1">MMKEPEKIIKKQHINGQLKYQVKWKGFDETTWEVEENVKKYKELIEDFNYFCLTGDRYDDKKLDEIRQITAQAQPRTAIKRVAGPRPPDPNKKDKKIDKIDQKYSEISETLSQSQLENGNNNNIQFSSNTNNNTQNNNENSISNISNQNNVVSNLQTALIVNIHQQNHKTSSIISLKQQELTQKVTQHYANQDDKLAMIKLQITEDNCVFQLQWKQRSDGITPLPEFYSYDQFKLQAPLFFIKFIETCLLECQTNDNDIKFEIAGKDLSEKTNLIKGALQKREERSKKINAQPLLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJ93</accession>
    <name>A0A8S1SJ93_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0100116</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJ93"/>
    <dbReference type="OrthoDB" id="282152at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13749">
        <property type="entry name" value="Zn-ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003618">
        <property type="entry name" value="TFIIS_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239:SF12">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51321">
        <property type="entry name" value="TFIIS_CENTRAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS central" evidence="6">
        <location>
            <begin position="118"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIS-type" evidence="5">
        <location>
            <begin position="254"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8141441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="296" mass="34904" checksum="77777417342D78C6" modified="2022-10-12" version="1">MDQIDLSQILKKLIEWKNSSPTTLYNLLDLIGTQNFGQNRIVHQKFLQAIQKLAQLNEIPPNSIYKSYDYQKVIDKAKSILKILRQSLQMEVEKEKPEPMKKYNLFAGYQQQFTGYVDRIQNLNLISNLILHIFKNWDQSNKEQLISIDKIKEVTIGIEKEIYLRNIFKDSKKMAYEMDIKTLVQFMKRDKTGDVLIRVFNKSLSYQQAAQLKSEDWIDEKTKLNQKAAQKEELEINQIGFYKEISKREMDGVEGKQCKGCHQKKVYLVDEKQTRCADEPTTKFFECFNCGDKFRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SMU3</accession>
    <name>A0A8S1SMU3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0090476</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8141170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SMU3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8141170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="180" mass="21782" checksum="5B68295362DEC2AC" modified="2022-10-12" version="1">MIEKEMSLNCNQKHKQRIEKVSSDPQLNKSDKLLCYTSLLNPKFDDKQINFNAIFQVIQDKYKFKKQQFENILNTNLGQIEQLQRDPCIFKIKLIQQPVKIVQFTNEWIQILNNFFIKLLNIVYLIQFIIQLEMTRQYLKINKIYFHKLINSINDNMLIILLIKYQIQNKRNCQSRILRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SNX3</accession>
    <name>A0A8S1SNX3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0110133</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SNX3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="177"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="155"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8142425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="330" mass="37993" checksum="EE2D6E6071E8294D" modified="2022-10-12" version="1">MSSTNRSYYQLNASNILSTKRVPSLTPTKNYPSSISKEKITKQVKDKAISGNSQQKSDVFNLPMQNALFLQQQNAILSARKATINLSDSSDKLRKISPNDKSPINPRLSTLQKNKQISISQSQQAIAQVLAKMQENDNISYMNKQLYHNPEIESTQKSTINSREYTNNSSKHKTEDYQKIQRENFILQRKVQELEQKISALESVNQQLLTLVKSCDCHQTQLRELEITICNLNYLKETINPIQIQNITSLDMQSNTDLKKKLSYSQDYSEIKPQNLFTNHNSTKMIEQFIEQHQLNQQELNVFKDLTKKMDDKKTHIPSVLKVLSLVINV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ST59</accession>
    <name>A0A8S1ST59_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0100248</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ST59"/>
    <dbReference type="OrthoDB" id="292650at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8141672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="185" mass="21785" checksum="8A6C65B1C55E6C7B" modified="2022-10-12" version="1">MRNKNYQSFEPFKTYQSIKLSTLIQDIHQAGRNLYTSSEHTSNFDSSLPCLKKGEQRKFVNRIYQSYQMEKKAVQQGLLTPQHLNKYLQPRKKHFDLKKQINFLKKTKFQTCIQKLRAANIILNGQKSQQGKNEIIQETLESKKGQQHDIEFIQKMNECQLQQRMGKLLKLKSSPFLFSNQITEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SUQ3</accession>
    <name>A0A8S1SUQ3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cysteine protease</fullName>
            <ecNumber evidence="4">3.4.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0140199</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase C54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.-" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SUQ3"/>
    <dbReference type="OrthoDB" id="433762at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019786">
        <property type="term" value="F:protein-phosphatidylethanolamide deconjugating activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035973">
        <property type="term" value="P:aggrephagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005078">
        <property type="entry name" value="Peptidase_C54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046792">
        <property type="entry name" value="Peptidase_C54_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624:SF49">
        <property type="entry name" value="CYSTEINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624">
        <property type="entry name" value="CYSTEINE PROTEASE ATG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03416">
        <property type="entry name" value="Peptidase_C54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase C54 catalytic" evidence="5">
        <location>
            <begin position="57"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8145351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="406" mass="48580" checksum="5BF64BFA0B765998" modified="2022-10-12" version="1">MDQRQENYKEVLIDWIYNLKYILNAYLNNKNVPPGHPIYILGHRIDIDNNDIEDRINKIKQLVQETIWITYRRNYPPLYQSNYISDTGWGCMLRVGQMAMAQMLKKHLKNHGDKRDDDYDNILLAFADNDSSEYKEFIEYLNKKDQQKVHNFICPFSIQKIAYLAKKEFNLDPGEWYKPNYILFLLEQLHNTIPIRASENLKLSVFNDSCLFLDQLMNRMFETKFDTNKDLEEQLEKTQLKSKNSLAIFVLTRIGLDEPNQKYLKILDEIMELPYFQGIVGGTPKRAFYILGRINDHYIYLDPHYVQEAENKDQIIENKMFNRTSYSCKYIHLLNQKHVDTSMGLSFYIRNQIELLQFWRNMKQIKQQSEDFFIFLSDFTPEYVDYSKQLEESNNKLNDDDVVFLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SUZ3</accession>
    <name>A0A8S1SUZ3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0120309</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SUZ3"/>
    <dbReference type="OrthoDB" id="289272at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF110">
        <property type="entry name" value="DEVELOPMENTALLY-REGULATED PROTEIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="382"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="9">
        <location>
            <begin position="641"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="133"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="230"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="138"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="411"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8143598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="875" mass="102133" checksum="BE84692C4BB10086" modified="2022-10-12" version="1">MKKLSNQMQKIVARNSFQDSPQKLEKKNIKAITGTVKTERPIEKAKAKLLDYFKRERNSLAQETNKIRSKLQGSKSSDNVYLQTLKSDRVKTSQPNIAEMRVQTEESCNSKKGTLKNKNLSLAAVSLEKKELAKHPLTTKTSYNQTPTNLNKPQKKKQQQKDQYLFDMLKNAQMIQNQYQKYFNQKLNKQSIISDASTNQRQSIDDILINQKVTKGTSQIIQSKIIRKTDNEQKSLNQGNSKTEQTPQPKQKTKTKNEDILEYDPKQKLQIILYYKNQKYYYLFEYANQSTCDLFNYLIQQIASIEKNYVQQDGGTGSTDDDQIQAEIKKICQFCTIQKKVPFDYYLSLPDMSLLVFQGNTLSLQPMYSQPQTTKKVGLKDFQLIKCIGVGGFSRVYLVRKKDNGIFYALKLIDKKFIIDNKKEIIVQNERDIMVKMKNQYIIPLHYAFESKFYIAFVLEYCAGGELFYHLRKLKKLNEQDAKYYFVEICIGMAYLHSQNIVYRDIKPENILLDLQGHLMLSDFGLSKPGMAAEDFAYSFCGSPEYMAPEMLMKTGHNYLVDCYCLGALLYELVSGLPPYYSHNTQEIYNSILTETIQFPDYVQISNQLKDLILSLLEKDPTERLGQKEGVIEILTHPWFNDVDFEKIINRQLPTPYKPESLKYNFDEEEFNKGDAEFRKQFAINMLNEFQNVDTAKYQLSNFYYQRDQQEEYNNRNYIVNVNKLQTQFLQKEPISPPKQIINKTKHSPQESKKSLKTNIKSETHDYFKKHNLLTKPSQIMQDSLKTSHGYSKTQQNSQISIQEFKKLKLLFDQSKQLLSSDRLTTIPDQNQKNQIERFKTEQFCNLPSPKTTTHSTVNKLNKYQKLFGSEKRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SV91</accession>
    <name>A0A8S1SV91_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0110230</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SV91"/>
    <dbReference type="OrthoDB" id="10659416at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8142574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="86" mass="10218" checksum="87C757AF7EE40527" modified="2022-10-12" version="1">MDLPMETLSQQQELFQILLGKSRTKRLINQCQRLILTKLINQQRSKFKLGFQMKLISHFHPLLKSINFITTIMLQQLILVKIHFVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SVD8</accession>
    <name>A0A8S1SVD8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Oxysterol-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0120104</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SVD8"/>
    <dbReference type="OrthoDB" id="14833at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000019">
        <property type="entry name" value="Oxysterol binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF148">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8143229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="428" mass="49160" checksum="46763A8C5FF5A45B" modified="2022-10-12" version="1">MFGWFKKKKPESNSAQQEEKDESDYVDDETQVVTNEDKELEAKGFDPIAQVQEQDKENEGIADPDKLEYVGYHDIPGDDILQLVLNFKYFPNDEDIRHPEGYEKGGRICADKKVVSKARSVGKEMVKQIGKKILSGSLNLTKVSFPIRVMIPKTALETSVHGTSVFPLYITKATMTPDFLERFKLVITATLSSFFWTNTFLKPLNPILGETLQASYNDGTQVYCEQIMHHPPVSYFLVYGPNKKYKYYGYYLVEGRAGLNSVTIINKGKRSIEFNDGQKIDFDFPNELYSGTFFGQLRQESTNKITFTDKANGLSCIIDIGKVKKKTSDYFQADVNFKGQKVSTVFGTYIGFINFDNVRYWDYRYVVPHKIKMDKQPLESDHKNRSDLQALKAGDIPLAQTNKEILENLQRNDRKLRESYEKQKKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SVN5</accession>
    <name>A0A8S1SVN5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Leucine rich repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0110320</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SVN5"/>
    <dbReference type="OrthoDB" id="304294at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050836">
        <property type="entry name" value="SDS22/Internalin_LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46652">
        <property type="entry name" value="LEUCINE-RICH REPEAT AND IQ DOMAIN-CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46652:SF3">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8142754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="307" mass="35538" checksum="76C4A83908377072" modified="2022-10-12" version="1">MGDIDIKENDKTLSLLNQNLTQITINKQSCNRSLQITNFNASKNSIKQMIGISIFHNLRVLNLSHNQIQKIEGLTNLKQLCALILNNNQIKLIQGLEKCLELNTLVLSNNQIINVQGISHLNKLEKLQLSHNQIEDLENCICMNLEQLNVNNNKIQEIPKFFSQMTKLKRLDIGKNDIKDPQQMIVLRDLKLIHLNIAGNPCSDKAIQIAIKFPRIRFINNLAAEKVLEQHLSKVPQKIREKTEKVQKKISQPMVSYDLEQEQEAVIIKKPKRSQIVKVEKNKQIDKKQKQRIKNINLDQETKIEKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SVX2</accession>
    <name>A0A8S1SVX2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0110254</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SVX2"/>
    <dbReference type="OrthoDB" id="2414662at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004838">
        <property type="term" value="F:L-tyrosine-2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006572">
        <property type="term" value="P:L-tyrosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005958">
        <property type="entry name" value="TyrNic_aminoTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01265">
        <property type="entry name" value="tyr_nico_aTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45744">
        <property type="entry name" value="TYROSINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45744:SF2">
        <property type="entry name" value="TYROSINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000517">
        <property type="entry name" value="Tyr_transaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5 6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="7">
        <location>
            <begin position="73"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8142622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="417" mass="47319" checksum="E76534DD4B126F37" modified="2022-10-12" version="1">MQQVERSWDMLKPSARAQVSLNPIRNWVEHVVPEVEKAGKQEGRPEKKIVFLLGDPTAYPKFQTPNEYKVTVANSVGKIDGYTDFFGDLKVRTQLAEVLSSHHRKLDADDIILASGGSGALFYATLALAKPGDKILMPRPTFPLVKAFADFYSIQVVFYDLNPGTWQVNIVELEQIYEQNPDIKFILVNSPSNPMGSELSPQALTEIVNFCERHNNLPIVSDEIYENMIFEKREFKFISDYAKTVPVLRCSGLTKKCLVPGWRLGWLALYGEGDTFKEVKKALRNISNILLMPNTICQAALCDVYKKSLDIIPEKMDELHARYKALHHGLHDAYGISVGETKGAMYSTLIINIEEFSDIKSSIDFAKKLQQEQNVLVFPGELFYGEKFVRLVICCDLEIIEEACQRIKKFCLDHKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SYL6</accession>
    <name>A0A8S1SYL6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cysteine protease</fullName>
            <ecNumber evidence="4">3.4.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0120296</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase C54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.-" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SYL6"/>
    <dbReference type="OrthoDB" id="2960936at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019786">
        <property type="term" value="F:protein-phosphatidylethanolamide deconjugating activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035973">
        <property type="term" value="P:aggrephagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005078">
        <property type="entry name" value="Peptidase_C54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046792">
        <property type="entry name" value="Peptidase_C54_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624:SF49">
        <property type="entry name" value="CYSTEINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624">
        <property type="entry name" value="CYSTEINE PROTEASE ATG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03416">
        <property type="entry name" value="Peptidase_C54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase C54 catalytic" evidence="5">
        <location>
            <begin position="47"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8143572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="312" mass="36151" checksum="41A1C500CE10149B" modified="2022-10-12" version="1">MKQKQGFGQSLMQMMTNTAFQLDKMLSEEIKIDELMYIFGQEIQNTEAFLQKKETLIWFCYRANIQFEGKAISDQGWGCLVRVGQMMLANALMRESKILNINKTKTIIIHLFDDNQEYSTIAPFSIQQIIKRASINLNMKIGDWYTGPKIMSVIEDLNKNNKNFKQIHIVNFLEQCILESQIDLSFKKPHLLIIHAIIGDKSLGQLEIHYLQSHMLISQFAGAIIGKHNKAFYLIGFQKNNAIFMDPHYVQESNKVEMECNLKCQPLKQLNGTIALAFYISNYNEYIQFKQQVNKQQGSIFSIIEDTGQYFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SZ54</accession>
    <name>A0A8S1SZ54_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0150253</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SZ54"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8146255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="231" mass="27776" checksum="4A61B401D0B98BBA" modified="2022-10-12" version="1">MILFEKFQATNLLMVQYDLIYEKQLQNHKMIYLKESIIKRSVQKRISIKRIRSLAKNQIKNFNLFLLLRFLNLGLINKNFICMLQQVYLLWLRTEKKCIPTINLAYSEMKVLKEQNIKIKQISNFSKDVLFKIKDINQTEDHYKANCLAINIQINKYYAVLKKGSMINMFNNRLFIFSELFKQNAVQILQEEGPFLILFKKCLSKFIENFVEQQFQHLLYLKNLLQVLKFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T424</accession>
    <name>A0A8S1T424_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0160243</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T424"/>
    <dbReference type="OrthoDB" id="309730at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="142"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8147170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="170" mass="20247" checksum="93112089842C8373" modified="2022-10-12" version="1">MDEEILDNLQVIEDDNEISNFPLFQQKQIGEFIKKVLDDNITTQKAFRDKFNRCLSLFKTLERKKMKKKRIQVTKDDIIMTLQAIDFQEIAQSLENVQLQQSSQENAIQQENLLDQGEEDDELREILYFENSEEEQKDEENNNNNNENIQDNQDQLSEQIDEENNENEQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T7T0</accession>
    <name>A0A8S1T7T0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0160225</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T7T0"/>
    <dbReference type="OrthoDB" id="9332038at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14210">
        <property type="entry name" value="PKc_DYRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000380">
        <property type="entry name" value="Serine/threonine-protein kinase ppk15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF22">
        <property type="entry name" value="DUAL SPECIFICITY TYROSINE-PHOSPHORYLATION-REGULATED KINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="284"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="69"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="597"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="641"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="598"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="613"/>
            <end position="625"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8147134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="689" mass="80130" checksum="2DACE77162C22627" modified="2022-10-12" version="1">MKSKCNPQDKKVTVMINKNNQNISFDLNSNNKPKYYISNTTCHKDNQKFFNCSNMSPIVVETSTPRKRLISGKRTQHKANSSNNNSNNKIQNRQKKSSSNQRTNLTVKTGSSIEALKELMGKNYFKQLLQINTDKFKNAKDISFIETKKSNSSEFGFDNRFNKNYQQLNQQGIKTTTNKSGSKDRYSLQKVFSKVIDQEKSSSPTKKIDWSKIKLPLTPKECIQQFGQYLTDFEKQEIHGFSRIYCIGLTSKKIDQKQTNYNDGFDDSKGEYLFSINDHIGYRYEILEIVGKGSFGSAFKVFDHKRQQVQCLKIIRNKKKFTNQALVELNILTYVKEKDEENCISFEFLSINLYQLIKNNNFQSLSLELIRRFAIQILNALNFLSKHKIIHCDLKPENILLKQANKSGIKIIDFGSSCFENQKIYSYIQSRYYRAPEVMFGIPYDTSIDMWSFGCIMAELYLGYPIFPGDDEQEQIAYILEILGMPDNEVLEIAQRRKVFFTDSLPFQPLCLQNKGGKLRIPGSKTLSEVLKCNDNNFIDFLQQCLVWNPKKRISPIDALMHSWILEGLPSQIRVQHVQYLESQQQYFDINDKKQETNLKQGQQGQQKLQNHHISEPEMNQENKKPNQIRTFSREKELLINVSQNPTPNNKKTSQSFHSSKNSNKQQLNFIQQLPGTTKYANKKTHYFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TA67</accession>
    <name>A0A8S1TA67_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0210122</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TA67"/>
    <dbReference type="OrthoDB" id="288603at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="362"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="370"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8151001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="537" mass="63360" checksum="5D8F773B204249BA" modified="2022-10-12" version="1">MGSCQGSTVDIQQDLRMREIMTKFQVPMPYIDEYKIFQTLKQVEIIQHNSIPHFDLIYSKMGDSSNKMKNLVKKWRMKHELHNHPNLLSIHNVSIVESMKLFIPYTNIILLIDKPLQPLTRHKKVILKQPFSQDEILFLLDCVVSGLAFQQQNKIYPSGFDIDDIITVKTPFNTEIYKLVDRYQKPIRSNLFQDFIQLYDDKQTLNELKKTAFFSPAQIEAIPKKDKYLKHNRYKSDVFNFALILIYLIQAKHPEIFDWKKWTIDDFALRKTQDDLLVTNFDENLLHIIELMLTVEEDKRPDFVELDEMVTKLFSDLQMQRLQEKYQIQDFFIIPIDKPSDFNIQILNDNIYQSKQPQQLIGSQNLSQHQRNDSQKVKQSDISQASIQIQKNEINNNLFDNNIVVTQGKLHYSNGFYYVGQICNRRRHGVGTYYNQDNQKVSEGSWVFDIPDGDVILYNHPDEEKEMNHINMSRKDWVQYKGQMKQGQKHGIGVLYFSDGSKFMGNFSFDQAHGRGQFEYQNKKQIIGTWQHDIFNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TBA4</accession>
    <name>A0A8S1TBA4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0180419</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8149108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TBA4"/>
    <dbReference type="OrthoDB" id="371850at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="47"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8149108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="348" mass="40787" checksum="48D52BDFC78795D0" modified="2022-10-12" version="1">MMEKQNQEIEIFDIEKQIIQMNQYEKSILSQHQIQNFIRSVIRIKDIQVLSHLGKGSFGQVFKVIYKNKIFALKQQLKQQVLQDVKYIQSELSIMKYLNHPFVVKLHHSFQTQHYLYMLLEYCDNGDLTNYMTKGTIIQETQAIFIIAQIILAIEYLHSLNLIYRDLKPENVMITKNNYIKLIDFGLSKEGFNITHTFCGSPAYLSPELLNGQGVTQSTDIYTVGLLLYEMLSGYPPHFSNNIRILLNGIQNEELRIPKNFSGSVKNFLEQIITKNPNDRLSIKQIKNHIIFAEIDWLKLQQQEYIAPKLKKKETNKLTQTTRLIDHDYPQDGERINEVFGWDYSCQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TNI7</accession>
    <name>A0A8S1TNI7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NF-X1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0230283</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="392140at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031380">
        <property type="term" value="C:nuclear RNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031048">
        <property type="term" value="P:regulatory ncRNA-mediated heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06008">
        <property type="entry name" value="NF-X1-zinc-finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000967">
        <property type="entry name" value="Znf_NFX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057373">
        <property type="entry name" value="ZNFX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF341">
        <property type="entry name" value="NFX1-TYPE ZINC FINGER-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25396">
        <property type="entry name" value="ZNFX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00438">
        <property type="entry name" value="ZnF_NFX"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="NF-X1-type" evidence="5">
        <location>
            <begin position="1095"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="5">
        <location>
            <begin position="1123"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="5">
        <location>
            <begin position="1204"/>
            <end position="1221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8152872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1535" mass="181815" checksum="D3CACFA8B11D4792" modified="2022-10-12" version="1">MYNDDQPSILQLENNVQDQELSLWIRDCFIQQFDIEKILYRLDNDKNIKKQLKYLNPTHQLFDYYYYLIQTMSEFVNINQNIKKYLEYIAQSQSFQNQISERCHLIITQAESIDNLEYQIEQLFQIFFLLKDIDMNILKQSNISQIFTLQKIIEINFPECNYNALYKNFDQFLCLLQDSAEMQKYNNIPSYPQPLEFIQFGGENKQMFGKTKNHIESTQNYLNYLFNFQREDYIYNIREDIHYLSEKGFYKPIDVDTIDLYQDIRLISFEINNFDILWKISLEQFNLDGRKIKLIDWNYSKKLHLGSLICITNVECHPLLFGIITHRSQKQDDYNIKQRINLQFRFLGSKSQIMEFLNLLSQQTIFMECKTSTQAESLIYQLESIKKIQSLNYIDSFIQKNLFFKNSSQNFDTFLQQQQWSQLMSTLDEQQQKAIQYIVYENLALIQGLPGTGKTHCAILAVRILIKNMLEKDKPIMIVCQNNHTLDYFLEKIIEYIPINQVARLGGSSESAIVQKCLFKTKSKIDFDQVEFQELKKCLHIIFNRLIQYDYTIDAQYITRFWPQLKDKLINDFLLKIDLNNSQIDDVAQNQILNSWINVKQLIDSILQFETQLFQVHQNFQKEQIIEQIELNNIFGQQTKQSFNQQDQDQQTLNFSLQNESPTSPFEQFNSQNQNESNLHYNFKGIEKIQECLQDKTLNFWELNYNDICEIIKYLKYLKYQEDCLLFEKIYRKFKKMSQTLNDLNDSFHIKKLNEYEIIGVTFSDAAYYNNILTQLNSKVLIIEEADEVLKSDITTILTNNVKRLILIGNEVNLANSFNSYHQKNIDLFRRLNPNTIFRENKIPQVILSTQRRMEARINDFVSMFDTKNYLDDQILIQISNKKEIIGLNSNIFIFNHYQLEQIDSKSKENAEEAEMIFQMVQYLIQVGYKESQITILSIFQKQVQGLKKKFMNQNQQQVKVETVNNYLGEENDIVIISLVINDNHHLYQKKKNQQKIKIAFSRAKLGLYVFGNFDFLQINSDKPQILYELIKDFKRLTFLEKLITLAEIKNFYSDTITLKCRTHGKQSYVQKSSDWFKFKAGCCDQVCNQNFDKCNHTCQEICHLGNHPENNCQEQCQRILQCGHKCQQLCRVQCTCTQMIYVTLPVCNHRALINCGQDPAEVLCLQDQELQFLNCKHLIHYQCYQREDFLYKCQHACDNLLQCGHLCSKKCWEICQPCEQKCVKRRNCGHLDECQNLCYQSCSPCNQKITIQLDCGKHSIQNKCFQIQNHILKEIQAPPFFLKSKIGSHYLIQIMQMLKNGQKEPNFIFKQVEDYKCKNPCTKPRKCGHIFECKNFCSQECTQCEQVITLSLECGHKYQLLCKDQNNLLQNQLSKNEQKILSQCQHQKNQLSFETYQSCSQIIRNQIGYKIKCQQVCNRQRQCGHIDPCINKCGQKCSPCQYIITKTLDCRHELQIECSSENLQCNRPCTKIRECLHYYPCSNYCSQPCSPCQVEILINLPCGHQKLVKCYNKENEQQRLVENEQNCEYCQNYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TRP7</accession>
    <name>A0A8S1TRP7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0240320</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TRP7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8153629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="189" mass="22557" checksum="4B6A0DFDE3AFC6C5" modified="2022-10-12" version="1">MKSITQLKVYVELTLASQVYQIKSNSHQNKLNRQQKIFWKNKNNKQIQDQLIIYKFKNLQHKSIDFTKTKASKFRKTNSIAIHFRTDIAIPNNISLSFTDQRQLGQEIYLKLLKLSIHFSQILNYRKTLNIQQTFQSNAIPKLRHRRRIMKQQKEGTFVNKEQIQNFFSNKNKLVKDVEDLSFDRGKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TUW2</accession>
    <name>A0A8S1TUW2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0280039</name>
        <name evidence="2" type="ORF">PPENT_87.1.T0280040</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8155918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8155920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TUW2"/>
    <dbReference type="OrthoDB" id="299318at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8155918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8155920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="80" mass="9129" checksum="58F42A101C80F5B7" modified="2022-10-12" version="1">MENSDQNNPPLISIINPKYSIPFNKPIYFKIKDLIAHKVNPIKPKLPLFLKNIHVTTNQKLFVIAVITTYVVQTTLEVIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TW53</accession>
    <name>A0A8S1TW53_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0270021</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TW53"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="50"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8155309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="128" mass="15616" checksum="AE8C46F03D2AF904" modified="2022-10-12" version="1">MKKDYLILQISYLIYVQLTNLNQFDQQFFKQYFEIVIVQLKEIQQNIYVKQKQKQQIKVLIQSINELNQKIHNEQQLKIKKIEILKFNKLQSSNLVQEMINETEQNEILVQEEDLHVNQILQLKIIWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TWY3</accession>
    <name>A0A8S1TWY3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0290072</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TWY3"/>
    <dbReference type="OrthoDB" id="299663at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8156594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="208" mass="24459" checksum="AF721213AE7544D9" modified="2022-10-12" version="1">MDERKKIKYKKPTKVKRWDEALLLSSSPENTEDEDVLRTEQEINKVTGGTNFTKADMEEIKQSPIYCVCNTDQNQTFYDWYNEAKIQKATLKTDLLVDVMDVMNNTDKQKSEKSKPVQKRTAQLAKKQMKLIEKKKNELMKQDKDDKIKTVRGKIKIDKEVFPGCYTKFFDKEDEDDYEDSQDNPIYSAIDADQIYQIIMQQKQAQDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TWZ6</accession>
    <name>A0A8S1TWZ6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0280116</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8156072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TWZ6"/>
    <dbReference type="OrthoDB" id="297709at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8156072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="195" mass="22872" checksum="77B4144FCDFB9587" modified="2022-10-12" version="1">MKKVKGGDFNFASRAQKIDKLEFPQSTEERFIVKANKDGVGFQWKTYDEKLLARTIDKQTFDNTVAEATRICRNLWREKQREEHKDPTKAYQPLLYVSVFLILLAFVFLLVLIYGNRDKLALLYVAVSILCFAALLTLIVVAKTWSLEPQFMDLEKVQMNKVTEYLNNQNSQIYQTKGYKWQVEPNLYWIELVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U6I0</accession>
    <name>A0A8S1U6I0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0340302</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Organophosphate:Pi antiporter (OPA) (TC 2.A.1.4) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8160370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U6I0"/>
    <dbReference type="OrthoDB" id="287797at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000849">
        <property type="entry name" value="Sugar_P_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43184">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY TRANSPORTER 16, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43184:SF12">
        <property type="entry name" value="SUGAR PHOSPHATE EXCHANGER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002808">
        <property type="entry name" value="Hexose_phosphate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="269"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="298"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="339"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="369"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8160370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="395" mass="43897" checksum="C6AC012DDF533E52" modified="2022-10-12" version="1">MAQPGLDNYFNSQYLGYINFAFLMSYGLSVSAIGQLGDRMNLKFFVLIGAFSTCLIFCSIGFFLHYEYKHPYLFMILQILNGISQSTEWSGLLAILNNWYMGPNKILILGYFSASPCIGNIIGDLYSGSLIGKHDLPYYAPIYMSGLSLFTISIINLFALQQAPFPNETEELVEHQESMSISLEPILNISKENSNDSGEYSLPSSHFFKESEQLSLSYFDAWFVPNVAMYAFAFGCIKSVYYILGFWLPEYLNRNQVPDVAWITAMTDIGAAPGGIIICLWIGTVIMIIINFADNLAIFGYLILVFIIGFLIGGTYNNIAALITVELSNQDQLKNNKKATATIVSLIIGYASAFSAINQLIVPLVNDALFLYCGIMSIIGGIVLSPLLIKEIKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U765</accession>
    <name>A0A8S1U765_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Malectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T0330223</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the malectin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U765"/>
    <dbReference type="OrthoDB" id="303132at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021720">
        <property type="entry name" value="Malectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039155">
        <property type="entry name" value="MLEC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13460:SF0">
        <property type="entry name" value="MALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11721">
        <property type="entry name" value="Malectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035830804" description="Malectin domain-containing protein" evidence="12">
        <location>
            <begin position="19"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="275"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="Malectin" evidence="13">
        <location>
            <begin position="21"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="312"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF11721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8159587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="350" mass="40261" checksum="512592BC28BF0D0F" modified="2022-10-12" version="1">MIQLFIIIALAFSKPLNPKKVLYAINCGAPSNYKSPSGIQYDKDIQTDPETVVADYSLNSEVSSQQIKYTREPEIYLTERHAYKTFSYQIPLQKEGTYTLILKFAEMYFDTKGKRVFHVKFGQQRVIEKLDVVAKVGKFASNDEYIEFEYIAGQVFHNNVLCVDAVQDGKLQMNLEKTKFDNPYIHGIVLYAGGINETDYEEYQYMKENWEKVINEEKIKEEVERQKKSEAKVKRKERVKIRNDHLNEVENEFEVDDQQQNTSIPVAKKGGLRSLITSPIGTAGLFISLFVGMSILNKIMSAISNAKIKGIEKDIITESKPKQEKQPKEKSDEKKNKPKDTKEKSKAKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U8R0</accession>
    <name>A0A8S1U8R0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0360230</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U8R0"/>
    <dbReference type="OrthoDB" id="286241at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8161525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="339" mass="40294" checksum="D41E472BB1EA414E" modified="2022-10-12" version="1">MLKKCTDNPKIAETLKQLVQERGQKRWPEIAKVLESMYDIKIEIPFTLKAIYQDIMNPRPGLNTEQLKLLVTTAMKYRSVIRLALPEFQKVSGIGISRHKFVSRLYRFYKNTILAGIQIFIRNKMDYRKRLAKFPGQSIGRILQCKELTGEDDFTQRLKLMAEQLEILLNCYLQLEDERDYSLLYDILTPKMNKKTFFLLLNCISFMDDLRCIQQNVIKSDDELMPDYWLQPTIKENKELLVYKKLFLDDMDYKFRMYTDSQDVQTEIDFKFFLNNAYMKPKSVVTQRSAKLKTVRGKITVNDKKYPSDKTIIFDQPEEESEQEGYEIDFKKHRLLNKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U9S9</accession>
    <name>A0A8S1U9S9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">USP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0350089</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U9S9"/>
    <dbReference type="OrthoDB" id="420187at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02257">
        <property type="entry name" value="Peptidase_C19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP" evidence="1">
        <location>
            <begin position="83"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8160643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="423" mass="50392" checksum="5C04CEF93A9DC538" modified="2022-10-12" version="1">MKNQFSYGSKYNSQIQQTPFGNSQIIKTNNQQSKSRLPIFDDYKIQQPQSFSQFNNFSIKQLQTQQKIDQTQQQQYNVVQLPKGLNNIGNNCFMNAIIQMLYSIKQFRKAILDLPVKQLQTDSISFHLQSLFQELNFSKIQYSINPTQFYHEILKKHNQFQFGRQEDAHEFLLCLFQTLGSECKNQQNEQSFFDDIKNFFEDIFLVNNRDLEQKIEKEWKQKRQLENNIITNLFVGLFSNKIICKNCQFEQIHYEEFNVLALSLEYYLESCLLNELIKFQFKPQIITNYKCQKCQQDEHQLIQKIAKLPKILIIQLKRFNYLNSAQRINTNIIFPLDNLSLIEQCTPNIRSCSYDLQTIIHHSGTCNNGHYYATCKYIENNSIKWFRFDDSIVEEAQLEKRQYDTYGTPTPYMLIFEQKQYAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UGZ1</accession>
    <name>A0A8S1UGZ1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0400306</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8164268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UGZ1"/>
    <dbReference type="OrthoDB" id="63989at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000131">
        <property type="entry name" value="Dual specificity protein kinase TTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001747">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="24"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8164268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="624" mass="73803" checksum="7D6E6FA6D4D31730" modified="2022-10-12" version="1">MNSLINNMAESDQFVIQAVFPDQYKTIVLIGRGSYAKVYKAETMNGQYVAIKVIDLTDMQKEIIPYMQNEIKLLSESDNRNVIKLFKSHQTKDRLILILEYCSLDVEIMVKQYFKGKLPDNLVIIILRQLVNGLSYLHMNKIIHRDLKLENFAIQLNYEDQIALQKNDLTVFERATYKLIDLGLAKKLGDLQAQTSTWAGTELNMAPEILNEEKYSFQADMYSLGVCIYYMLVGKYPYLDPTNRIPLQDLIKKENADFSQIANQQLKNLVQKMLKFDPKKRLQFQELYEHDFFKYREGDLSNPIDLELNQNQAIYSQQMDESIMDNFQQISQKEEIKQQNDLENKFQQVIIEEHQQQINNYIKNQESDIIIIQSKVENSQEINNINPVSIYDNSKAQDNQIMQLYHIRNQYNTILKLADYVEQMIPLVKVTKLSFYSMESNFNMYIEYLRMVSKQLFIKLQQKFTFYQPTLGSYDRYKILERQVKGEDQKRLENIRIREGYSTGIISWLKKSIFKKIQCEPSDSLSFNQQYQSINIELYKELLRMLQDSFYKYQDNSDPKLKKIKCLLYLSMIYTARSRVLFNVEQQNIDYKAEELLLAKAREEPDILSEKVTSILGEYKLEYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UH17</accession>
    <name>A0A8S1UH17_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0370126</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8161956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UH17"/>
    <dbReference type="OrthoDB" id="291146at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001267">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="9"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8161956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="539" mass="63688" checksum="9A995BA550F0321D" modified="2022-10-12" version="1">MSSKVVENYSLLEVIGSGQYGKVYKAVNIKNNSLVAVKVVKIEKFKEVPKLEEFTMNEIQTLARINNPYVVKFIEMLKSSRNYYFVYEYCNGQTLEAVIQEQGVQTEKEALYYFRQLVQAFQSLIQDNIMHRDLKPSNIMLHNGQVKLGDFGFCKALNGPQDLSSTMVGSPIYMAPEILKGQEYSIKADIWSLGCVLYELLYGICPFEEKTMAQLMLAVEEREIQFLDNVNVVSQTTKDLLLKMLTKDPNKRINWKELFERELTYAERTKNNEQYTNQIQQELTLNSQRNKESKAFKYLLMERNKIFYLYKVVEEVLELSKEERDETRESQSIFCAYLILKYICYLIEMIRMNLVDKFNVSAFPHLVKKQELQNDITKTFEYKSFCTLIQKEVETARKMFKHYQDEADKFYKSQNNGQHIYQGAEFSHFESEIQRELQSSEISITFMKKIVLKYCEDLKSQFLQQFLDKNPQLGNKQLLHIEKTLESLILDEFFENCMDIQQIDLEQQNYFTILNKYSKGDLLQVITNKMDYIRHKLYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UIR9</accession>
    <name>A0A8S1UIR9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0400299</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8164254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UIR9"/>
    <dbReference type="OrthoDB" id="282796at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8164254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="189" mass="22024" checksum="6C610AAE6E36C8C5" modified="2022-10-12" version="1">MAARQVYCRLQPKVNNYHNDGTGRDSYISKFNGGQMNQTLQSQIAPQANHDFEKMHYLTKSNSNLGRSGNLPPQIPIKTTKYWGDGTGRDYFVIVNDGGQCNPCYWWDNVDIQFQRQLRNYQKTGPLRISSACSSKQQDRLAEPKKQLQIKQRPHTPNIKQAHKIYQSYQTLKYQSKARNIRYKTYNDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UKG9</accession>
    <name>A0A8S1UKG9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0410265</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UKG9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8164884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="412" mass="48222" checksum="0F20B4300DDA0488" modified="2022-10-12" version="1">MRNRFQACSFKNSTNSLIFNTPLTLNKTRSCQSKEFGYANKKMIYVTIILPTFQPYQNIISKLKNQNIKIQQRKQIIMRNKRHLIQKKTILEIMQKQEQQPKKRTNRHHNTVFYNHQASSPTQPNLCSNDTNSNPSKENSRHSIQLHRYNSLSPPKLYIDKRTTKLQTFSQRLKTFYLPITAPFDSQTILKLEMIQDGRTKVIKQQGSLDDFQVNIRLYKFEDDDKYTDEQGKDKQTKLFTLNQEEDEEEEQIDNDNLRNQKQLSNNQQKQAYKNILKFNRRSLQSLIDKQQLGKSRIKEILTTEYSQRAITDNLSPMNNCILGYSPISSIKLLDEKRKRPVHKLLPLDINPVSQFTFSNMIPHLPLSIISPSIQSTKNSNAKRKLTRKIINAGGVKKHKKNITFGQLLIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UKL0</accession>
    <name>A0A8S1UKL0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0400328</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8164312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UKL0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8164312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="159" mass="19306" checksum="A49DC601769CFCB0" modified="2022-10-12" version="1">MNIQTIFTGKNYLNVVIQLCEHHSETNMTYVSTKKEKPVDQKNFQLFNLVDWLKVENMELNILRGTKESYDEYTEDTYSTQIIKSEINKEGDIFEFEIQIQKFYIRILFIRNQIRIYIRQIENTLNIIFISQQRKVRNKRQQCLYLTQQINQSIEFIFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UNQ8</accession>
    <name>A0A8S1UNQ8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0400220</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8164096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UNQ8"/>
    <dbReference type="OrthoDB" id="305792at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23327">
        <property type="entry name" value="RING FINGER PROTEIN 127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23327:SF51">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR OF YEAST FORM ADHERENCE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="346"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="12"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8164096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="364" mass="43005" checksum="D0A65A0578E4AB7C" modified="2022-10-12" version="1">MKLQKIYQECKCPSCQLLFEEPITIVCGHTFCRECIVRSVNLKPQCPECLYPITNIINKIQENFLVKSVVKEINELFIEQNKKKVKPRLFDQIIAKQFQENSIKQYLIFETKSLIIPYTNQNVHLNINQFKDLNEDQIYKILKRDSLILLTNPNKNHSIQETATLVIVIKVVINSKLIDLSVQVKEQMKVLQIKPETFRIEKEIPLNVAKVQEINEEPIFFTLQTDQQIKYLIDTIRQQLASHLPQLIDSYTAKYFEEFFHRLQLFEILRSNILRNEKALKHISFVIPCILHLTDQERTRIFNANNSIERLIQISQVLQRFQNITNPLMVFKIPGDKERINNKTELIIIVLLLFFAFYYRVIGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UXR3</accession>
    <name>A0A8S1UXR3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0510052</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000051">
        <property type="protein sequence ID" value="CAD8169445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UXR3"/>
    <dbReference type="OrthoDB" id="10255449at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8169445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="499" mass="58167" checksum="78C2FD828406A20A" modified="2022-10-12" version="1">MEQEVGKICLVEVPIEKTFLFQDQITIDKNLTTLREGLSYRLAQSFMHPQQKLFIVCPPSYALKELDTKINNKTLFDGKGYIKAVPKADYPSLVGQQSGMRSAIIQQNGKRYRLKGCGNGNDGFTLRKFEGDIENAFEIRGCCFDHTVKRELFMNRKIEQILKKYNIETGNISIGYWQYGDIESINQNLEKNLQNQIPLVPKYCGIYETIGDFRLGTHLLQGLRLIVNSNIADWCVDDIRRLVTVQSRIVENRKSLNGNPWDLNNTQYRERPEGTILQEDDYQNNRVFDWEKEKDNINIIENLIGNSMLQNISILYQRIGYEVGKIKRIFIDNDISWGYFRDHSKFYYHSNAHLDNFVIIQNGSNVLAPVDFDLAFCKEEFINIDIDFDAQSANSSEFSENKNYGKFDLEQWLFYQDQERIGLELAIGGMDMILASHMFIEKQKTDVEEFFEILLRDQMRIGYLEGMQLKEDSSLFYQRMDKIQDIIKEALNLTQQIVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UY54</accession>
    <name>A0A8S1UY54_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0500182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8169143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UY54"/>
    <dbReference type="OrthoDB" id="307868at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8169143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="280" mass="33078" checksum="335B9B743FB4C873" modified="2022-10-12" version="1">MISTPNCIESKIYEIQTNTHTPKSEHEEFKQILIKLWIQISKSFNEDFEEILQLGINSDIQDVVNAILIIEEARLEQIKSDRYQIQLLKDEIQFLKEQRNINSGNDEFKEVQQQYRELSELVIQLKQSNEQKDHQIMEQTNLNQQLQIELNLLTSQINNLSSALVESKEEKILYMEQSQKQIKQLMEKDLQQNKMIQILQIQKIELENMIKKTNQRNFIESSNHQISQTQREEDQARILSCSPRTTHIDFSKIVLQSLQNKKTEMVSGQSLTQIPYRFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V109</accession>
    <name>A0A8S1V109_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0530052</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V109"/>
    <dbReference type="OrthoDB" id="298898at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8170424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="757" mass="90762" checksum="6FE4C4CD593376EF" modified="2022-10-12" version="1">MIFKIRQLFSTITEPATGGRQWLKFFQNSTGIQINIERSLKNEATENLNYYVRQNYKTFNQEQLFQAYIKKDLQFEIQKQVQMEVLERFVADQMNFSENMLITYLNKEAQLLPAFYSVIAQNLLLNGLKIFDQLNIYQQIEYMRLCQQAGFLHPDIFQKFIQNLKIDDEFIESLSLLQFRNIMTLFDYCATSPYDLGLQPGFIDYLCNKYIQKLSNFELVASEIMKYEFSQLMINHHFSFLSEEQYSEIKIAYAKILERMFNKNYNFVVHRNLNTKDVAKKLLWIYKPDDFKLIQKSLNHLISNTIQQGEFKADHMIYALPLYFKYFNPQLYYLLLKQCHKQNLNFKISKYDLKQIMYLLNSNALKGLRSIMADHTILDDVDNFIKNTLTSYVNNIKDEELLNFEEIFNQNMEQEAELDFNDAEYAEILPYFQNNNKNQETQNHVNQRQFIVNLTQKFKSLQNTLYKNGFQRISNQKINKRVSILEPILSYIEVLYYVSIFNPEFQPQTKLFKTPQILKLVNNLIYLPNLLENLIQINQIWPNLFNEELKSIIIANKKKIDQVYNTCQDCQEFIDKVEGQIKLTEQGKSRKLQYRNKNYQGLLQAFNFARPKPHNKLSPILKNPFEEYTIEETYSNQDFNIYWKLTQRSENVIQHTHFKELMWQALKLDKNQWIMNYSDFPHLVDFACTKQKVVVFLDEYPRLIRGLHKVYTIETERAAEYFSRLGYQVVFIDYNVYHPDNEQMLNILKQKFVFRYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V1P7</accession>
    <name>A0A8S1V1P7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0530199</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V1P7"/>
    <dbReference type="OrthoDB" id="306134at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8170694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="65" mass="7798" checksum="EEC0EA446F94F1AC" modified="2022-10-12" version="1">MSDQQQLKQEVLSNEVYKTCFKILPVNASQDEFKKCMGNYIQSYEIIQQAFEQFFKKPKFPQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V3L5</accession>
    <name>A0A8S1V3L5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0550064</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V3L5"/>
    <dbReference type="OrthoDB" id="291626at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001816">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="17"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="414"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8171515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="466" mass="55319" checksum="3F47B29EE4F77972" modified="2022-10-12" version="1">MDQKNNLQPGLIIESRFKLLKKIGSGSFGIVYLTYDLEEKEYCATKFESRNLQMRMMNREILILKQLKGINGFPELIHYGKDSQYQYYISTLLGENLEVLLQKCGGKFTLQTVLQLSIQLIDRLEVFHSMNFIHRDIKPENFLISKEDTSLVYLIDFGLSKYYRVTDGRHIEFTQKTGVIGTARYASINTLQWMEQSRRDDLESLGYMLIYLVKGELPWSNVKAVDKDDKYEQILQIKMGLSLNQICFNLPKCFIHYFQHVKSLLFQQQPNYSHLRNLFEKQLLEYNHQLYDWELKTIEKQQQQDLQIDPNSIPDIRQQNDLDIIPPVDEEGQVIRLKQEYQNHVKFMKQLHEKYFDHNSQKQIQQIQLQKTTSIEHKSEATSKQNNYQNSNHTLIQIESQTYIHNSVQQFSKRKDSKSYTSFKQSQEHKENRKFRQSNDDYDLDMANEDAANFDQNNLEVNYFSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V431</accession>
    <name>A0A8S1V431_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EML-like second beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0560146</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000056">
        <property type="protein sequence ID" value="CAD8172198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V431"/>
    <dbReference type="OrthoDB" id="1068471at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25173">
        <property type="entry name" value="Beta-prop_WDR3_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="257"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="299"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="341"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="383"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="425"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="515"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="557"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="599"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="641"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="649"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="683"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="725"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="767"/>
            <end position="808"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8172198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="885" mass="97229" checksum="C0F502D27505CCD4" modified="2022-10-12" version="1">MHDSFQVKEAIAEIIIQVKDVDEKIFGVILEIFKKEKVQDCIGYLSDDENHKHLEQQIFQQSDNKKKQCEIIKNLKQINDVLKKIKDHDFNNQDFSEESKLSLIQNIKDDKQNKEFLQFLVHLTSIDETLIQAGSNSLHILVQMKVDFRNKNFENIKIQNISLVGATFVQCNLSGSEFNSVNINGMNLNGAILFNCKWNNLKIHELNQLVCQTYYARSVCFSSDGNTLVSGGGNGFGTYDDSVCLWDVKAGQQKAKLKGHINIVYSVCFSPDGNTLASGSQDQSIRLWDVKTGQQKAKLDGHTSGILSVCISPDGNTLASSSYDQSIRLWDVKTKKKQAKLYGHTSTVYSVCFSPDGKTLASGSADKFIHLWDAKTGHQKAKLDGHTNYVMSVSFSPDGNTLASGSADKFIRLWDAKTGQQKAKLDGHTSEIMSVCFSPDGNTLASGSSDNLICLWDVKTGQSNAKLEGHRSAVYSVCFCPVGNILVSGGGNRFPTYDGPIRVWDVKTGQQKTKLEGHSSAVFSVCFSQDGNTLASCSADKFICLWDVKTGQQKTKLVGHSNYVYSVCFSYDGNTLASGSADDVICLWDVKTGQQKAKLKGHTNIVYSVCFSPDGNTLASGSADDFICLWDVKTGQQKAKLEGHCCTVFSVCFSPDGKTLASGSDDNSIRLWDVQTGQQKANLNGHQYTVNSVCFSPDGNTLASGSSDMSIRLWDVKTRSQKAKLDGQISCINSVCFSPDGNTLASGSFDKSICLWDVKTGQQKAKLDGHTSTVLSVCFSRDGKILASGSWDNSILLWDIQNGKAISSADKIYKDILAQFKAPLCLNNPQPESNNAIILRISQIPLFQAQGALILKGDFINHEGYDLKQLFKSKGSCFLEDFKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VBQ2</accession>
    <name>A0A8S1VBQ2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein kinase PLK</fullName>
            <ecNumber evidence="8">2.7.11.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Polo-like kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0610051</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.21" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VBQ2"/>
    <dbReference type="OrthoDB" id="408964at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13118">
        <property type="entry name" value="POLO_box_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13117">
        <property type="entry name" value="POLO_box_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14099">
        <property type="entry name" value="STKc_PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001669">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.30:FF:000013">
        <property type="entry name" value="Serine/threonine-protein kinase PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.30:FF:000016">
        <property type="entry name" value="Serine/threonine-protein kinase PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000091">
        <property type="entry name" value="Serine/threonine-protein kinase PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033701">
        <property type="entry name" value="POLO_box_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033695">
        <property type="entry name" value="POLO_box_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000959">
        <property type="entry name" value="POLO_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00659">
        <property type="entry name" value="POLO_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50078">
        <property type="entry name" value="POLO_BOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="32"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="POLO box" evidence="11">
        <location>
            <begin position="438"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="POLO box" evidence="11">
        <location>
            <begin position="538"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="333"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="617"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="360"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="389"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="619"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8174364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="639" mass="72357" checksum="628FDA5908750075" modified="2022-10-12" version="1">MDDKTNQEGAEQLVIFEEKITTVNGQVAIKKYQRGKFLGKGGFAKCYEATNLETKRVLAAKIIVKSSLTKNRARQKLISEIKIHKSLQNTNIVQFEHVFEDHENVYILLELCSNQTLNELIKRRKRLTEIEVQCYVGQIINALKYLHAQKVIHRDLKLGNLFLNKSMELKLGDFGLATKLEFEGEKKRTICGTPNYIAPEVLDGRVGHSFEVDIWSLGVIMYAMLIGKPPFETPDVKTTYRKIRLNSYSFPEHVLISDPAKSLITRILNLDPTQRPTLDEIMAHPFMNTGGTIPKTLPLSTLACPPSASYNKQFQPSTNTSNLKMSTNVLPQRLTETTPNNPKNVQKQTNGSSDRFPLQKPSSSGNIMDDNFGSSGLNNAQNVGYGGTQRPQSQKPNDIRNSQSQKTLSQPFGGTGMQGAQSVNNLGVKQTQAKQEVYVKKWVDYSSRYGLGYLLSNGATGVFFNDSTKIVLDTKTLQFEYMERKGQDKQDICETHNLNDYPQDLHKKVTLLQHFRSYLEGEPQTAQSEENENKQLVYVKKWMKTRHAIMFRLSNKIVQVNFTDKTEIILSSEHKMVTYVNKTGERSHYPLATALDSQNTEMSKRLKYTKEILTHMLNGGQNAGDSNSNQRVNANWQNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VFG3</accession>
    <name>A0A8S1VFG3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0620200</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8175155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VFG3"/>
    <dbReference type="OrthoDB" id="305216at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="168"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="62"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="176"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8175155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="236" mass="27962" checksum="4224DD1B510DDB90" modified="2022-10-12" version="1">MQENQICQTDQKEENRQIKQKLLEYWVKLQQALDEDYEEILKLGSESDPNDIINALFIIEDARQEQQKLDKQQLEILTKELQQEKEKVIELQQQNQHINQCNNKTDNQLETRQMEIKINELSLALVQCKEDKIIYMEQAQKEIQYLKEQELELKSKIQMLQYEQQKKSSFLDNEDDSKRLSKDFADDDIPRQTTSPQIQKVKLDYSKLSQGSNFKKLTKVDQVQGISQASFKFGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VGH9</accession>
    <name>A0A8S1VGH9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0620057</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8174909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VGH9"/>
    <dbReference type="OrthoDB" id="308281at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8174909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="198" mass="23461" checksum="42A60D8F4D0164A6" modified="2022-10-12" version="1">MSTSRKLENEYNHCIYKMMYTLAKRCVKAITGESVDHQKFAALIGKQFGIITKLEEERSIQFKFSESLQELTELLGLEENNDIIRNNQQKQKVQIQQQPKRLELSRKNLNPVKNLSKSIDQNTVKQVGIIRPESDLRESKESRDSSNVKEMKEINQNIIRNSFFIQRRSIQTENGCQKTLEQKEREFLERFRQRLEDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VHN6</accession>
    <name>A0A8S1VHN6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0640145</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8176053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VHN6"/>
    <dbReference type="OrthoDB" id="9332038at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14210">
        <property type="entry name" value="PKc_DYRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF22">
        <property type="entry name" value="DUAL SPECIFICITY TYROSINE-PHOSPHORYLATION-REGULATED KINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="152"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8176053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="562" mass="65553" checksum="95C345FC85A6F031" modified="2022-10-12" version="1">MKLADRKKFSVHLDMSALKQIIQTAHPLSTKRKIDTTPTSPNTSGLVSDKYFKPVQPLHTNNNLNNTNTIQNIILNFKNKANKEKENFSISTMTKFPLLIDQFIRLFNLSKSELLEVQQMKQVYYYKQSKIQQDGQNGEYLCYAKDHIKYQYEIINLIGQGSFGQVFQVLDHKTQKTFALKIIRNQDKLKKQALVEANLLMMIKERDPQNKSNIVRIEEQFIFRGHQCIVFEKLEFNLFEVLKQQKFRGLDYETLRKFSYQILVSLNYLHKLNIIHCDLKPENVMVQDMKSKIIKLVDFGSGCIDGNQVYTYIQSRYYRAPEVIFGLKYGMEIDMWSFACLVSEIHTGQPIFPGDNELEQFNLIMEVIGAPTTEFALKCPRKKHFFDENGQPKKTIKTYRKPQSISLQEILKTTDEDFIDFLQKCFTWDAESRLKPQDALNHPWILSITPKENSSKLNNKFFNDTDSNSAFKKSKTQTNTILKEFEKKLSKDNIFLKLQQTRTLIANNTFENKPVLSNRYVNDKPTSSDRIRSNIAHSIHQILSCRQHNASNTQFIRKPSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VJU8</accession>
    <name>A0A8S1VJU8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Signal peptidase complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0670257</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Dispensable for SPC enzymatic activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SPCS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000067">
        <property type="protein sequence ID" value="CAD8177534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VJU8"/>
    <dbReference type="OrthoDB" id="263893at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005787">
        <property type="term" value="C:signal peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009542">
        <property type="entry name" value="Spc1/SPCS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13202">
        <property type="entry name" value="MICROSOMAL SIGNAL PEPTIDASE 12 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13202:SF0">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06645">
        <property type="entry name" value="SPC12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8177534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="94" mass="11124" checksum="9B93424969DDC7B4" modified="2022-10-12" version="1">MAEGQSDTFWKSLYIYFNEMDFESQLFVKKIYKIIILTVAIISFIIAYFLERFSVCVYSTIGASVLCILVLAPAWPMWKKNQPNWQQANWQKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VKM8</accession>
    <name>A0A8S1VKM8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0690237</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VKM8"/>
    <dbReference type="OrthoDB" id="304414at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8178518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="350" mass="41850" checksum="7F2E6A4458FEA374" modified="2022-10-12" version="1">MQLKQRKRIDISDDPLFFNDEPVLKLRKLKLKKMADLETLIHSQNQRRESFDQNLNCNKLLQQSKNQSLDGGKIIDYVKVQNRELNQVKLQQKFLNRSRILQDEPQKNKEKELPFVEQYLFRKTVKNIIKRSHIKHDNIRASLNKVETFLSGIDTTRDQYPQISPHSQTKNTQIKTCRNEDNSCHSIKSQQFNVSESPLVRDRSKSLMQTIEQNNEIRNMRRAKVNEIIYKTEETRERSMAAKLSVYSEKPDSIQPTKKQLQQLRYKWVRKLKYKIELHNNQMKQFMDFLDYLKSSDIKVKSEDQQYLKYYRDKLLNGDHLIEEDFQMMINDQNTHIIGLLIRGYIAIEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VV12</accession>
    <name>A0A8S1VV12_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0770022</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000077">
        <property type="protein sequence ID" value="CAD8181688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VV12"/>
    <dbReference type="OrthoDB" id="301196at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8181688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="317" mass="37342" checksum="0BF45DAB30314E8A" modified="2022-10-12" version="1">MIKRNQQLKKQIAPSRNQNNKACLKPQVFQFPQLMNKTSTFTEAIRFQGQIQQPFTPDVMIRQSLNKPRMIEQPKSKNYYGVQRFSAKIVSKQQNVKEPVIIKITTNKNHKVIQQLEEQSEYQLSELGTFDDLIQQIQERVLAKNSNILQLAQDICQIETIDLRGLQCIPQLIKHLVISSVQQQLQISQILSILFTLSEEIMTRDDLDESLMEIIKVYLQKGYESNLQMIVVFLKRCGQMIYYIRANKQKVLSELIITLLIKFNDCNQFEESQLRLAFNIILRHKQLRLYIKDNQDIYKLKQPSLIDQLQRFKIKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VWQ1</accession>
    <name>A0A8S1VWQ1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0750018</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="EMBL" id="CAJJDO010000075">
        <property type="protein sequence ID" value="CAD8180763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VWQ1"/>
    <dbReference type="OrthoDB" id="9909019at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="8">
        <location>
            <begin position="82"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8180763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="320" mass="37705" checksum="24D9565BFF149423" modified="2022-10-12" version="1">MIKKYLNKFFIYFICFSIVFVYIVNVKVVWIDLIESSSYRIPYLLIFTILTTLLGWSLFKTMFTDPGRVPQNWGFFLNDPEQKKRKFCLICHIFKPERCHHCSACNRCVLNMDHHCPWINNCVGFQNRKFFMQMLFYVILDSYCAVIGLGYGLYVEFKNITLYLNSEGDLHFIDALLLLCAFGISCLASCLITMFFKFHLELVLSNRTTIENLEKKRNEETGQQNDDFNQYDLKPYYNWVQVFGMSKLSWFLPIQMEGGRPVGDGILWPKNHHNDSLLLKDHVSNPIYNGNGDNFQMQQYNNQLLHSNNQQFRSSSTQHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VX44</accession>
    <name>A0A8S1VX44_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0700121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000070">
        <property type="protein sequence ID" value="CAD8178816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VX44"/>
    <dbReference type="OrthoDB" id="302010at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="483"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="487"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8178816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="504" mass="61078" checksum="1156DBC5609AA28D" modified="2022-10-12" version="1">MKYKKFLTTDCLNQIVKNIGKAQIRYLQNRIMIAYQLKLEFVGLYITFQEDSPFFQIYFGPNINNKKQDYHEMIKLIQDRLSQPFDNFNPIESKKKILGLFSQYNDNMKIKFELVWKYIQFAYILFNEHKWIMNDEINKHRLYSYFVRDYDYVTKNELVHPSLWLKSDSFLILPALVNQSTFFDYIFSVDSDKQKKVLKEYNKISNQLAIYLFEIRDILQEFAKQKLQIQIYPSLLNFNFIRIDNQKPYYLNHQHFERFVEQNSKVYAETKKNISYFEEYLEKNNQIQLRIFNFYPSDEIGSGLTFVQNKHREEYPKEFQEYWQSLYKQKYPSKRDVESLQIKAVSLEYLHSLNCNVNSSFDYIQHIIDPERMICKQCKQQIDDYGSLFYDKTFYQFYKCLKDILLSPYYIDIPGVYICPISLKLIGIKNNFMISSQILNQKGEHLITLYNQTLKFELREEFDAELEDDDEYYEIMNGIREKNQEQTLDDFNDDEQDEAKLNEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VYW1</accession>
    <name>A0A8S1VYW1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0720015</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000072">
        <property type="protein sequence ID" value="CAD8179396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VYW1"/>
    <dbReference type="OrthoDB" id="294299at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8179396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="308" mass="36450" checksum="DAE6AC3A9EB1A110" modified="2022-10-12" version="1">MSKELVNLKGNSVQLKMDMPIQQFRYFTSIIQNNILNEWITTKKVDKIKEKLQQFISDTYQSEYIIIIAVKPAYNLSHKKNRYLMIKIEDITIFIYQAKPVDYFQFSQIKQIQTKLQGVTKENNVSEPILISQKNISEQEMDSLQKVKLCLNEIIILDYENWETDLVIAAKSKLHQLFQTGFWHIYCAKKITTLVDLKTNERYLEFQFDKKNSQYKLIAFQKKGPNFNLLEKVKLKIREIISFTVLFIFFVTLTSCKDETSGKQYKYQIQEQFCSHRQEVIGFCAIVLFGLVAQRMIGKRNQKKNKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VZ07</accession>
    <name>A0A8S1VZ07_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0790207</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OSBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000079">
        <property type="protein sequence ID" value="CAD8182924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VZ07"/>
    <dbReference type="OrthoDB" id="14833at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000015">
        <property type="entry name" value="Oxysterol binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF148">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8182924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="881" mass="103485" checksum="E02CAF12D988453A" modified="2022-10-12" version="1">MDIRSTIYLPSKQISEGEIQVKGKTLGRWKTKKFIIDEEQRIFALKSSQAKKKLKPFYLQYYKLEIREKKKDKISFDLISKAGGKSVTLGIQNEQQANVIINLLQKLITKEEPIQKQQYEESQLKFQTEIPVENTQQSAFEKIDYEIPCFFDTSEIRENISQIRKGQQRLERFQVVYKQQTCTIYKNVENHHQFITFIEFNGDCCDLIVQNLSDNNKINQWNPYVSQTESIVVKELVEDKSFQICEIRQLTNLFVFKREYQYLRHHLKIDNIEFIVQKQIDQRPKTKCNQGSLKYGLWSIEYKNNVTKVCYVTEQSVLGLSYPDEDSYLTQVFLQQITNINKSYIQKTTKQDQISTTVEISQINKVITLEQQVQGGQSQIQKQQTDQQQLLSQNKVAQENQQYNQEENDEFFDCEEEDAIDEMNDKLYIVETVHNKQLIQDDVVKDVYEQNARRQSILPIQIDIQDNNEQEWVEKKMKLVLTGGYCSLSINVKHQLQPKEKGQQRQFLTSQEGGHYIFRRDFIREEKNGGLKVINEEKLAAQKAVIKFLITRIGASLLMGKSITGISMPVSIFEARSNTERVCSSLGFAPIYLEEAAQSQDIYYRIKQCAAFQFGFIFMYISCEKPFNPILGETFQGFYDNCPIYCEQISHHPPICAIQMYGRQYKIDGQLELVANFHSNSVVGRNVGTFKITFENPHQEILLTFAPGSLNGTTYGDKVLNYLEKQFIIDMKNKIILETTFNPDKKYCQYFEIEYVNQLDYLCGALCDVTDSAIMRYQKEGHRKYKGLDLKNDIKQVRHKIKGIWINELKVDNQKLISIQNDYPVKMQLAQFPIPSDSTFRMDILWWKLRNFDQSQQWKEKLEILQRQDRKQREQKTKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VZG0</accession>
    <name>A0A8S1VZG0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0760010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000076">
        <property type="protein sequence ID" value="CAD8181212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VZG0"/>
    <dbReference type="OrthoDB" id="4405280at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8181212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="77" mass="9102" checksum="AEF3A4DB269FC947" modified="2022-10-12" version="1">MYLNYQMQYLDMLRFLYIKAIMHFNQYESKKASCSTKSETIQSNCDNTVITHQVNKATYKNYCALDRTKQGMQGSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W1G7</accession>
    <name>A0A8S1W1G7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0820025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000082">
        <property type="protein sequence ID" value="CAD8183874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W1G7"/>
    <dbReference type="OrthoDB" id="283949at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035730870" evidence="1">
        <location>
            <begin position="20"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8183874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="77" mass="8618" checksum="728BB6AF60B2AAB4" modified="2022-10-12" version="1">MNIKLIFIFLVLLAQGAFGVVTKVTECQAKCDKQVNGCIYSCAQTQLRQSLVTQERCQKLCAGVNKECKNKKCYLDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W4V9</accession>
    <name>A0A8S1W4V9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Poly(A) RNA polymerase mitochondrial-like central palm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0800232</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W4V9"/>
    <dbReference type="OrthoDB" id="273917at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031499">
        <property type="term" value="C:TRAMP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043634">
        <property type="term" value="P:polyadenylation-dependent ncRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045862">
        <property type="entry name" value="Trf4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092:SF48">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092">
        <property type="entry name" value="POLY(A) RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="2">
        <location>
            <begin position="308"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="155"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="190"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8183392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="625" mass="73143" checksum="0C209AD9FEEEAF32" modified="2022-10-12" version="1">MQNQSTSCQMNECKNNETIKGTKQFRSKNNQIDNQKEKSQLECRKDSEGTNQGNSKAILKKKNEEFDENTCIKNIMSLSLSIINEELQFNCQQQNQKEQISEQCKVIQNCDQNDIDSFQLPPTAQQFPTQKQKSHKKAKNRKNKTDSSLKLPKNQTLTQKQYPQQPQIQQQSKEKQHNQLTPQGSHKIKQQLTQQKQKKQLSSTQIENCEQENKKDSADFDTQFSHIGARSTTAGNSQSESDDCLQQSYENKQIELSAQKLSSREKTKKKNYNNNGEKVGDKQYPHSTQTTPIKRKQLNIVKSKTYDIDELSNYIQQKSYQNRIRKQICLNRLNYLIYTNYHLNMYPYGSFETGLDLEISDVDVGIWGSQNLTYIQIVSFLQLLNTTLKQTPFLIKSKLIQSHMPILKLELNPKSSFYDDDQHIKQNWSYFHLDQNDQGKIIQVDISWIYQWSNVYNNPHLGFASTTIIKDWISRFVWYRDIMLILKHLVKSKNLNDAHTGGISSFCLSIMLAAIYMCKHYTQNDKKQILLDFLKKYGTQFDPLKEGIYIDSYGQQHPFIALEECPPYNPLTIYSPINYQVISQKVHRFLEIQEEFKQLYEHLSKSNKIQDYFDLPLQVNQPLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WA08</accession>
    <name>A0A8S1WA08_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0850213</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WA08"/>
    <dbReference type="OrthoDB" id="25896at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001129">
        <property type="entry name" value="ras-related protein Rab-44 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8185673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="233" mass="26649" checksum="FBA53141D2FA10A0" modified="2022-10-12" version="1">MTEPKAQYDELYKIILVGDSSVGKTSFLIRLTKNVIKKQSQPTIGVEYAAQSILLADVDKIVKSVIWDTAGAEKYKAITTAHYRKSEGALLFYDLCDRNSFDNVLSWRQEILQHTDDKIMIMLIGNKLDLLQDNPQNRCVSVEEVEQICQQYNMLYNETSAKEGTNVKECFEQLIRKIYESKSQNIEEEIPGQRYEIDDIKSEKKTQEYAQPNILAVPIKQNKDTSDTCCGTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WC73</accession>
    <name>A0A8S1WC73_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0860166</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000086">
        <property type="protein sequence ID" value="CAD8186115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WC73"/>
    <dbReference type="OrthoDB" id="295797at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8186115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="110" mass="12389" checksum="079261345BF3325B" modified="2022-10-12" version="1">MGCCQNLPLKETETIVMPQLSIKQEFSPKTLQMEPFSLNLIKGDIDCIERLGSTCADNILGEKQQSRVERQQSVQIKGEVIIDLRQAPSPSLLNKDRSSRNLNNKFIGML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WLZ5</accession>
    <name>A0A8S1WLZ5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycoside hydrolase family 5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0940123</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WLZ5"/>
    <dbReference type="OrthoDB" id="1887033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052066">
        <property type="entry name" value="Glycosphingolipid_Hydrolases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31308">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31308:SF3">
        <property type="entry name" value="ENDOGLYCOCERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035729513" description="Glycoside hydrolase family 5 domain-containing protein" evidence="2">
        <location>
            <begin position="16"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="3">
        <location>
            <begin position="58"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8189631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="483" mass="57097" checksum="05150271CFD7A64B" modified="2022-10-12" version="1">MQIYQTLILIISVFASPITLNERRYFQDQNGAIRIFHGFNVVYKQAPYLPKTEGFDYMESFSEEDCQILSKNGFNVIRLHVSFEGAMPKRGVVDEEYLDKILMIVRMAAKYNIYVILDAHQDLFNRQFCGNGFPDWAIQKTDFPFPKTMQMRFDSKGYPILDDCYQQKFIFFYSTNDIGRNFEMLYSNTNGLADYYGQFWERVAEKHKNEWNVIGYEIMNEPAPGNFQNSMVDYIWPGHANKKYLIPFYQLIHNYIRKVDKEKIIFFESDFLDVLGAGFDQNIGGQEYENKEVFSYHVYCGIEKISSLICNRIHQLMYYLKMRNVNDLKIGGFLTEFGAVGDDQKSKDILDFILDKADSYQQSWTYWQYKGYNDHTTMSTMYEEGLFYEDGSFQQLKLSALKRPYAYQICTDFIETNQYYSETREFFLSFKTKVGCQTKIFIPNEINSSQVFDYKCQNCQLLLIQEQHYEVVGEGNIELYFQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WMW4</accession>
    <name>A0A8S1WMW4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">P-type ATPase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0940212</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WMW4"/>
    <dbReference type="OrthoDB" id="294083at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF19">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="88"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="349"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="813"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="846"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="893"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="929"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="958"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="5">
        <location>
            <begin position="784"/>
            <end position="1019"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8189795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1071" mass="124660" checksum="3D64C0F23E7DD8C3" modified="2022-10-12" version="1">MQSLDGSSRDIINSIDSANKITAKQFMKQTYADTEARIKINKLQDLPPEETQNQSQIDQQGKLVTIKSTLTINALKEKSKFQIIIESFLYGFANFPLLYYIILLIASIQYQVIGFYNQLYLGLQIVFCFLITFYDTFMNKINLYRSIEQADQQQIYVYIEDEFRECSIGQLQPGHLIKLLPRQKCPTDGILVGTTGVSENIPLLEIGGQTITRRAGVANFNVGRQLEGKLEFDSENKKVRVTMKMETNQFCLDFQQQNKIQILQANNSFDEIFILVTGQTLQEQKQEQSSAYEKTKINPKLGFSLHIQNIIIILFTLLLSVILLAAHFIQTKYLYLDDKVNEEQMKTYLQFLCTFSWGIPIMQIPLMLIINRISKFQAEHDRLIDVDISGKRIEISNSNALANMARAKFILTDLNLLTRQSLVVNNILLNKQSDEINLNDLVTVTPIEDIDDLNFCQQEAKFALLSFQTNKKSEEDIATQKRILDYGLSLGINGTQRIIQDQEQNQQYFKTKFEVVTQKYLLIGYEQILKEDIKQYLIVFRSKFTDCKDLLTSEIQLQITSLLDAKDNLNQMNIIFRKIVSMQYLDKITNMVNQHKKIDDETLKQIKSDVEPVLFIEIIEDFYDCSQEIMDIRECQYKIWINSHIKDPQILESDPTMVGLFEQTTPQIMICQNGDELQVALDQIEKNSQIKQFGKEVNIFMNERPVLLIIDEATLQTGLKNQLLNEKFRNFFLKIPLVLLLNSKPHLNYLLSSIIKSHELICITNYITTTPSMQICLENQTETAFDIKMPTFSFLPKILIVHGRLNNIRQGSAISLINYQSFILFFIQFVYVCYNRFSQNYLLNEFIHFIMMFFTICYIFIFFSMAQDFPFEILRSQPVYHLMVRTTKNPYKMLLTQFIQGGFHGTFIVISVIYTFYGSNDQNGYVHGYQLQQFMILTISLLVIAFKPIIDQYKIVKSQIYLAMAFIFITVMIIYQNNEQMAYLFETGVKTKDLCALVFYPICALSPNLIGQFIQRLLSSNNLNQCLYKMEQKHEEDSENQKLRDAKNYIRLYQKEELEQQEDIVNSKKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WSX6</accession>
    <name>A0A8S1WSX6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1000044</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000100">
        <property type="protein sequence ID" value="CAD8191857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WSX6"/>
    <dbReference type="OrthoDB" id="2310150at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005399">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150">
        <property type="entry name" value="HYPERKINETIC, ISOFORM M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150:SF2">
        <property type="entry name" value="HYPERKINETIC, ISOFORM M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="3">
        <location>
            <begin position="16"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8191857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="349" mass="39679" checksum="7E934349E9D773BA" modified="2022-10-12" version="1">MEYTRLGNTGLLISRISFGNMVNFAPEDEEVNTEIIKTCYEAGVNFFDTAEIYENGKAEEQLGRSIKKLNIPREKIIVSVKILANLEQGGNPINRAFTLNRKHVIEGVNASLKRLQLDYADIAYAHFLDQDIQVEEICRGFNQIIEDGKAFYWGTSNWKPSKVQEAFNYCDKHGLIRPVVEQCIYNMVIRNLVEKDYADIFSQGYGSTIYSPLQGGLLTGKYNNNQIPEDSRAGKDNGWLTKDKAHAVFFAQFLGNDDAMTKLKKLGDLAQSLGYTQTQLSIAWVLYNKDVSTAIIGAKNVQQVKDSLKAIELYKKWDQELEQKVEAILQNKPKQDFNWQTGKPIPDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WTA3</accession>
    <name>A0A8S1WTA3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1000129</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000100">
        <property type="protein sequence ID" value="CAD8192007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WTA3"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF51">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="483"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="5">
        <location>
            <begin position="69"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8192007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="586" mass="68354" checksum="6B3FA5EDDAFDA540" modified="2022-10-12" version="1">MIIPLLLLFPIINAYFEQKLTRNVYQYQQFKINEKVTAIAPKKCPQDLLNYTHSDTYVSQICFNKNVYYNVEVIFGDNVTKNNTFQLALDLTSPWTWYKSQDCMSCKKINTLTYEIDDDCKHNSKNNNCFNDQFNKSFNAKKWNDVKVHGQIYSQNSLIQGTFQTDTIQMLAYNSSSKMTISQYISYEWGNSTPPSLVSITGLQMLQVQLINNELPILADGVIGFGFGYTETDEERAKSDADFVEKLVQEKTKLNLTKQLFALYTYESAMNFSEMVVQVGGIDEKYIRKQKSNGTWIDRLEKSGYYWMVEINKIELKNLEGRDVINTELPIKKAFFTLNSQFIELPYDVMKALTLNLQQFHSNTACDLVDSDMYILYCKNLPKSIQTDYILTFTFGNNQISINNSNLLYRECNSSSEDNRIWDCLFNIKFSQSEYVILGEPFMKNHYIVFENAPGNNTRKIGVFQAATHMYYPENPSQYEWPLFNAILFIFLFGLVSVCSITFLKSLCKDIFRSFKYRKAQNPEDQQSLRISKDIDYVDYSTQEEVQSQMKNIEMSDQKKIEEWNKQQEQFKFGEQFTNSLESNSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WUZ8</accession>
    <name>A0A8S1WUZ8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1040186</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WUZ8"/>
    <dbReference type="OrthoDB" id="27140at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000061">
        <property type="entry name" value="GTPase-activating protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000120">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF337">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="71"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8193613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="481" mass="56941" checksum="08BA093388B4D77D" modified="2022-10-12" version="1">MNNNIGEDDDDLLISGARNNKKGQINLDLSNYSCNIPDITPSFDLNNFKKYILNENLNINEFQELILNCQLSSMKLRFSVWRLFLGIFKINDSLEQKINKLNQNRLDYQNLSKQYLQAETKKESKRSIRNPLLQNQQEQQKPNVWNNFFEINHLKSEIKKDVDRTHQDKQLFQSLKIKNLLSNILFIWSVKNPTISYRQGMNELAANVIEVYFTEAQGFNNLEDNDDKKQIAIFFDTKFAEEDIFQLFEQIMNAHVDMFKHTPESQKKQQLIIQNRIQKIYDQYLKIIDITLFKHLKVQDVELSVFLVRWIRCMFTREFHVEDSLKVWDAIFYDYYLTEDKQWLLLVDCIVIAMFVYVRNQILEKDDPNACLKRFLKYPPVENLAQLIQAAFSIKNVLQSANPEQALLQDQFLITFLGSRDVSKSPKIFQNVKNPEFPDENNKLLEAINLKQQDTIEVQINQAQLLLKALNQEIQYRQINQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WYE7</accession>
    <name>A0A8S1WYE7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Insulin-like growth factor binding protein, N-terminal</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0970176</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000097">
        <property type="protein sequence ID" value="CAD8190906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="319522at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035820804" description="Insulin-like growth factor binding protein, N-terminal" evidence="1">
        <location>
            <begin position="21"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8190906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1123" mass="131728" checksum="B574562957B7492F" modified="2022-10-12" version="1" fragment="single">MINFLILFILGLNHLHYVLSSYVQRLNEEQQYQTYPFNSGTQNYNKFDAFSYGVWSKYLPLSIISQVGLIGMFDSHCFLQNSIIENTNKEINLLYYDCVDPQTNTIEKKVQFLSSDNQMYNYKYEINPLVYENYWYFFGLNIVPSEGSFGFFIFQGENLITSEQLNIQGPFKHQDLTIVIGGGLIVEESQILWIQEIQKLSYFPGKLAILNPSIDNLQENYDNFDYFKYFQQMDNPECISKTNSLKNLPNFDISKLNHEVFASESINCDEFQLETWIQISNIHREDQSFIYQLLKISANFENHYVKNENLSPFQLFYKFSNSQINCDYIQLFISTQWSINNIKLWHFLYVSLRNKVIQIQITFYEGLDQTLYETQFLVHQFHEVQFKLQYGNILKSSTNYLTRILKNMKFSNSASEVDIFQGCHHSCEQCEGPTNKDCLSCSKESKRIYIPQFKVCVCPFDYIDDLECMNTENYNLSLDPEFEYHSTSNCPQGQLNKDGFCLKCPSFIKDTIITCLECIQNPKGWQNDPYCQTNLYIDKQGSPAQYKIDNLKTYYIFDGIDIKPQQLGLYQNRYKYQYQDKYQDQFDSVYLDFEKTNENFQRFYSSSKKTFESYFCNLKQCEICIMLITKQICKKCSNISRLRNGICEEIVRGVIKKNNCQAPYYITSENLCNLCEIENCQFCFEYSSNDLTKCTLYSEFQTFQKDEFLKLGCALCLDGFIFDFTLGICIYKESTLPNCKRSFINPLDQQLCTLSAIQDFGVAPEITNCQKYISNCKQCIQTPQSIIKCIICEDGYSSSLTTGHCRICQVEYAKLWIEGDFTKGDAWMQQIQSFLMLFLPNKYMYPKSANFLHITPLAIKCIDKYELNQNFCSLYCDLNCVSCKKEEQSYKGFTCSKCIQDYSMEPLRTIQKETCVICPLLCLVCQQRTTEEIQNINPAYEIDDQNRKYTYKCIQKRQDQNIIIDPYQQIAKYCLNGICDQIISYQLYNNCEDLSKIFDGWRSYTDDLNFEYFNQIGTQKFNLIIPIQDLCIADKLYEESLTNELKKGIFSLFWVELTFLGNHSPSTYQPMIEIKNFDSISFKKLTFIIFQTLELIIINDDAPTNLILTDSKFMANNQDQIXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WZL8</accession>
    <name>A0A8S1WZL8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1040189</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WZL8"/>
    <dbReference type="OrthoDB" id="304710at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8193619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="127" mass="14967" checksum="8F3D7407B33E8972" modified="2022-10-12" version="1">MDHFEQPSAKLLYLINEMNRLNMITQLEKCTLKQFVLQEHQGIYDLLKQYPKSDTELDLAEAIIILLRGAPNSEQQQVQDDSEYQADLQIQEDLQSPLGNQLMWRKKGQQKKNKHQDNKPFELNKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X0W9</accession>
    <name>A0A8S1X0W9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1100137</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000110">
        <property type="protein sequence ID" value="CAD8195784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X0W9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8195784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="41" mass="4905" checksum="4F77FB56E7E6A7CF" modified="2022-10-12" version="1">MFENDIQNEESLKRKINLQNKNDDKANENIQNSQDQSELIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X3R9</accession>
    <name>A0A8S1X3R9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Insulin-like growth factor binding protein, N-terminal</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1030005</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000103">
        <property type="protein sequence ID" value="CAD8192906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X3R9"/>
    <dbReference type="OrthoDB" id="300641at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8192906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="404" mass="45932" checksum="E1DA495635663DC9" modified="2022-10-12" version="1">MVWNNAIYKKSWSISKPHYAVTIRLNLTYGDQYNGNFYYTIQGIKSVPHPKPSSGGQNFIGKNLNEITKYFEIFQSPFTSSSLDIEFQCTDTTADPRDQFCAISDYFIVVHYCLPFCQICNDGVSCVIWESGHTSQNCNTNQFLQFDSNSETYSCITCNQPGCLTCKNLEECTSCDFSKGLNNGVCFQCNKFCETCKGSLKTDCITCVSDFQRFIFNNQCQCLPGYYDDDINLPCHPVCGDLLVVDGEDCDDGNNNPFDGCHNCKYGCDAFCQECIQGVCYLCQNDLQIINNKCVGICGDNITSHDEQCDDGNIISFDGCFECQYQCQAECTDCKFGLCYDCSIKGWQLFNNYCIPYKFKKFYYIKQTIAKIVNMSKQMKNKSLNRTIIFLGKVNLQILQKQIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X617</accession>
    <name>A0A8S1X617_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1120113</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000112">
        <property type="protein sequence ID" value="CAD8196410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X617"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8196410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="95" mass="9745" checksum="414D5674A038AE9D" modified="2022-10-12" version="1">MAKGAKSRSQSKSGKKSGSATKNIKKQVQAASPAKVDVTVAAPKTPVASAKKDSAKKSTQKSTQKSTQKSVKKSAVKNSTKKGSAQKGKSGKKAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X6J9</accession>
    <name>A0A8S1X6J9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1140061</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000114">
        <property type="protein sequence ID" value="CAD8196997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X6J9"/>
    <dbReference type="OrthoDB" id="302653at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="60"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8196997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="189" mass="21968" checksum="BC79001296700F12" modified="2022-10-12" version="1">MGTITIGNCQCQCYINYVSCDCSCDQNEIRTNRSNRFTEQSTRNRSKKRSPGDSSDRQSKFSSTRKPTSRSKSSNSDKSIYLLSDYCNITTESLKQKKKRKPHPTFIKSRSLYIFEADTDKNLSNNVEQSSNPLSLSDIGTVNRPKMQSMDKGVLKKPTEQVQQKKYVKWNLPQHYLRQNKQYQQEFYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X6M4</accession>
    <name>A0A8S1X6M4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EML-like second beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1140001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000114">
        <property type="protein sequence ID" value="CAD8196881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X6M4"/>
    <dbReference type="OrthoDB" id="414519at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002141">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002195">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="313"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="355"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="397"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="439"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="458"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="481"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="523"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="565"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="607"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="617"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="649"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="691"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="733"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="775"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="817"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="859"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="869"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="901"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="943"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="985"/>
            <end position="1026"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8196881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1106" mass="122706" checksum="C80ED7E672855214" modified="2022-10-12" version="1">MHSQQHNQHYNKFQIYTKSQLLFYRNAQDTFINFAVFNFKLEQQKFNINNKKKMWCNFEDKESLADIFAQVKDVDEQIFGLIFEIFEKERVQDSIGYLQDYGNQSLLEQLKIKGENITQAEKKQKLSQCVQNDLKIILNLLKKMKNHNFNKKDYFSENNMRSINNLIKSIKENRVIIEFLQFLVHLTSIDNTLIQSGSNSLQILVEMKVDIRKKNFENIKIQNTSLVGGNFAWCNLSGSQFDNVNINGMNLNGALLFNCKWNNLRIHELNNIKSFTYSIQVVCFSSHQNILASCSFEEILIWDVKTGQQKAKLDGHKYGVASVCFSPDGNTLASGSYDKSIRLWDVKTGQQKTQLNGHSKQVNSVCYSPDGNTLASGSDDESIYLWNIRTKKKVVKLVDHNNAVQQVCFSPDGKRLASGSDDCSIRLWDTKTGQQKSKLDGHTRTVYSVCFSTDGNTLASGSYDKSIRLWDVKTGQQKAKLDGHSNCINSVCFSPDGNTLASGSDDKSIRLWDVKTGQQKTQLNGHSKQVNSVCYSPDGNTLASGSDDKSIRLWDVKTGQQKAKLDGHSNYINSVCFSPDGNTLASGSDDKSIRLWDVKTGQQKAKLDGHSNYINSVCFSPDGNTLASGSDDKSIRLWDVKTGQQKAKLDGHSNYINSVCFSPDGNTLASGSDDKSIRLWDVKTGQQKAKLDGHSNYINSVCFSPDGNTLASGSDDKSIRLWDVKTGQQKAKLDGHSNYINSVCFSPDGNTLASGSDDKSIRLWDVKTGQQKAKLDGHSNYINSVCFSPDGNTLASGSDDKSIRLWDVKTGQQKAKLDGHSNYINSVCFSPDGNTLASGSDDKSIRLWDVKTGQQKAKLDGHSNYVNSVCFSPDGNTLASGSDDKSIRLWDVKTGQQKAKLDGHSNYVNSVCFSPDGNTLASGSDDKSIRLWDVKTGQQKAKLDGHSNYVNSVCFSPDGNTLASGSDDKSIRLWDVKTGQQKAKLDGHSNCINSVCFSPDGNTLASGSDDKSIRLWDVKTGEEIISSSHNCLAQLKIPLQQHSQISEASNYITKLLISQSAIFQAQGALVLKGEFINHQGIDLRPLLKSKGSYILENQLDCNKKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XD24</accession>
    <name>A0A8S1XD24_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1190133</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000119">
        <property type="protein sequence ID" value="CAD8198702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XD24"/>
    <dbReference type="OrthoDB" id="10254720at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555:SF170">
        <property type="entry name" value="FI18122P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="1">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8198702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="357" mass="42725" checksum="37AF9247175D4099" modified="2022-10-12" version="1">MQDYIKIIDAQVMEESLINYTQYQIKGVRKGEEFLINKRYNDFVILKEVFNRTWPGCFIPPIPQKKSLGNLDSNVITYRSKYLSYFLKKILKIPYLWLGEEMSIFIKGTIEQLQEMAKNKTLQQIISKYEETFTFNDNQQVYDIDQFYSSTCHIIPMIENFSTMAKNLMKEKETQNSGFVMFVEHFLPQFEKLQQQNHSVFSNQETQEQFQLSKKCCTKNEYNKVYEYTKIVERELIACKDALIQRNTIVQKVKDEDEKLYKLNIQLQELHSKTLLFGKKEEKVAAIQNQIQQQQLSVEQLKQLLFLINKLISQQEIPNFYRNTKEKYSRLIKVFSDFQIKYHQAQAEFWEKIQLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XEF5</accession>
    <name>A0A8S1XEF5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1210168</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000121">
        <property type="protein sequence ID" value="CAD8199415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XEF5"/>
    <dbReference type="OrthoDB" id="302468at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050222">
        <property type="entry name" value="MATE_MdtK"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43298:SF2">
        <property type="entry name" value="FMN_FAD EXPORTER YEEO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43298">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN NORM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="214"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="259"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="308"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="341"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="381"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="413"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="446"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="494"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="506"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="522"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="550"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8199415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="573" mass="64826" checksum="D91992916CD17A22" modified="2022-10-12" version="1">MSQVHGSSILNKENPNKAPDHLGSNALTDLLSSTFYTVIGYVCLDIVFGISLHFIAQHNDPNAVAGLGLAFTTVNVLLIPMALGTNQSLNVHSAQALGAKKPKLAQSYFTLTIFIHLMYFIPFSIILILIKPLIAKTINEDDRDATSDSSQIYLYYLLPSTLFAILYECMKSFMIANKVFAVFMFIQLGTAILHILWCYLFIDYLNIGGGVAGAGLAIICTEVLNCVFCLIVICATKYKKQVFSDYKFKFPLRKKEKKLFASFLRGSIPIIGHIYADYFVFFLLNFIAVGFGSDQLNAQLALSNTSNFYYKFPISLSLALMTFVGNEMGAKNIKRAKRYSWVGLLLYIGFTIIFLSALTVFKQNWAEFYSGGRQEITDLILEVYPIFVFGFFVIDGLQGTLTGILKGIDRKDFVTYSTLFVYYLIGIPLVVYFAYDLGLGQKVYGIWFAFGIVNAILAILYLILTFTTNWSNMSKQICQRIEDQHKMQNLNDSLNKDFEEDPENLKKKRKVKSSKVQQSKKKKDENQVDSVEKKPKKERKQRRPADDEQQYSLQATEPTKITKQEVDTVPIMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XEZ8</accession>
    <name>A0A8S1XEZ8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1210182</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000121">
        <property type="protein sequence ID" value="CAD8199443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XEZ8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8199443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="207" mass="25301" checksum="3E2881AD7FF05798" modified="2022-10-12" version="1">MQMQKQQRLQSNRFKEYCQYTTYIQQWKNQVKLLEKDRTAGHQQIFKVAQIQNGKISLTMYYHKQSNCQQQFQDNKINLNNLQCYKNFLFDQNEFILSIIQQGNSYVLQDYYTRISFHLRYPFIKYTLQAINKNKNKKEKFIRDQLQICKQIQFLSQKNIHLITNIQLTRQGLIIYLSYQIISFVEQQVIQRFDDEYELGHQFNIKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XGX5</accession>
    <name>A0A8S1XGX5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mitochondrial cardiolipin hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1250017</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the phospholipase D family. MitoPLD/Zucchini subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000125">
        <property type="protein sequence ID" value="CAD8200560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XGX5"/>
    <dbReference type="OrthoDB" id="5205528at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016891">
        <property type="term" value="F:RNA endonuclease activity producing 5'-phosphomonoesters, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09171">
        <property type="entry name" value="PLDc_vPLD6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000078">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051406">
        <property type="entry name" value="PLD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43856">
        <property type="entry name" value="CARDIOLIPIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43856:SF1">
        <property type="entry name" value="MITOCHONDRIAL CARDIOLIPIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="6">
        <location>
            <begin position="104"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8200560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="355" mass="42381" checksum="10EE56DB3CF3BEA0" modified="2022-10-12" version="1">MNKERQINSKHSTADVQHLFFPNEDNFSRFCRKLKKCKSTFLGCIYLLTHQSIIDIIIQLATQGCRVDIIMDQNSEEFEERKQITINKLLVMSGFKVNVSLIESKGLMHTKYCVIDNKLTILGSANWTYQAFSNNFEHITIIKDSKTAKQFTEQFKNIWDQAKQAKFIESQIIYQPNQNCIEFKQKNRISPRLRISKRKFQMNFRKYKSKKQLKQKNKQNIKEKEKEKDIPKFQMIQIEQNQIQIQQIEEQKTKQVINQKELKDFLENLKLNEEKKQYNINQNTINNDAIKDTKYSLIQQESNPWQIQQQLFDPNIINNQVKRNETQNKDQNIIIIDEDDDEDVQIITDISKYFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XH39</accession>
    <name>A0A8S1XH39_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1230141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000123">
        <property type="protein sequence ID" value="CAD8200139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XH39"/>
    <dbReference type="OrthoDB" id="297768at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12621">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN CHORD -CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12621:SF7">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8200139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="529" mass="62272" checksum="6D4DBC2C14D8E824" modified="2022-10-12" version="1">MFKNFAQGIKQIDRFGVIFRPSVIDINPEYKSIFGGIATLLLYGSCLAYFFYQIIQWQRNTLLPKITSIQTSQSSKYYYMNDIISSFYMRKNYRKDEIDPFDPQNIILQPILSKFKNQQLLESKSFKYDQKSSRYNNTEIILQDLELNLNLENTLDNPQIDYILSFGTCIQEFLLEGQRCANKTLVDIYMQQHSHALLMNNYVKEYNPKTMKLENVKKQSLTMLNNDTTMYFQNQIRISKTKIDNGFLFPSETQQEFPVDIIMISQSIDIDSFQTIFYRATYLVLAYSLSETSQEQFIEYPKLSEVLADIGSIVSIILVLSHLCLLINEQKLEKECIQKIITMYYPETVNIKFKYNWYGKIIDVLQNDRSIDKVEFLKYYNEIKRVAIKKLNIANIIYTQAKLQFLIQSTYSQIQIKQAHKVGIRLKSFPKLQDQTNIEENQKNIPIGNYQIHDISNMESTDQNDNIISSSLKKISLNENPKFLFIKPQNDCHILNDEDFHLFTFGESIIKDNELNKQDCYFEKNYIDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XPZ8</accession>
    <name>A0A8S1XPZ8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1330006</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000133">
        <property type="protein sequence ID" value="CAD8203057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XPZ8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8203057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="80" mass="9339" checksum="4451D34C1D56B3CF" modified="2022-10-12" version="1">MSIYGIYQKFQVGQIISIVQMLSIHLLLNTFFLQVIEQLIFQIFKGGINQDVSIIFKPPSDLKIKELIPYLLTMFKYHSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XS77</accession>
    <name>A0A8S1XS77_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1340049</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000134">
        <property type="protein sequence ID" value="CAD8203538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XS77"/>
    <dbReference type="OrthoDB" id="1688044at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003958">
        <property type="term" value="F:NADPH-hemoprotein reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06207">
        <property type="entry name" value="CyPoR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.990.10:FF:000023">
        <property type="entry name" value="NADPH-cytochrome P450 reductase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384:SF17">
        <property type="entry name" value="NADPH--CYTOCHROME P450 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="2">
        <location>
            <begin position="11"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="3">
        <location>
            <begin position="201"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8203538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="582" mass="66758" checksum="956A4B6223301AAC" modified="2022-10-12" version="1">MQQTIDTREIINVYYGTTSGNSSRLAFQFADEAIQHKFIPKIFNLKEFNPDVFIQTRLNIFFVSTYGVGGPTSDAIDFNNWLQSKDRKQNELQGVHFSVFALGNTKHEHFCGMGVKTDARLEELGATRIFQLGKGNSCDETTDNDYSVWVESGLFAALESLSPLITVPANQVQSSIYKIKEFDNIDVLIGETLSFQAQKYKDSYSLKVIEIKELRKAPSAGNSTLYLSLESLNIPYKAAQNIAIYPENTEEYIQEICSMLNLDPKFIFQIETKGNHPFPTPISVHNYLKKYCDFLGPITKKQLYTLSLLVDDPKVKEELKFVSSHKGREQYDTNYLQTRECFFNLVKKYNIKNIPLQQLIELCPLISPRYFTIASSPLKYPNKIEIIASELLITKERLGLCSQFLRNVKVGQEIKCYLQESAFTLPQNPQAPILLLGIGAGLAPMRAFIQEKDEFIEQNKLDQSPFKGPMTLFFGCRTMNDYLFKEELEEYEKNGTLNQLKVAFSREGQQIRITNYLDVEFLNQFLESGGIIYVCGSTQMGRDVQIAIENMFKQIRKVMPYLAYRKVNELEEQKIFVKELWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XU37</accession>
    <name>A0A8S1XU37_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1360057</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000136">
        <property type="protein sequence ID" value="CAD8204152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XU37"/>
    <dbReference type="OrthoDB" id="10567222at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050349">
        <property type="entry name" value="WD_LIS1/nudF_dynein_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44129">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="128"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8204152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="155" mass="18752" checksum="361505668062A306" modified="2022-10-12" version="1">MVPLSFNKIQSQVPEKELLVLCQEYIKRIWMIEKDVSVRAFLKNKEIIEVQNQLFSQDLKTFSSSIKEEMNQKLKEMEDIEYAIRFEGNQSERDELSKQLTNKYEEFEEFLDNISEMYQQLNMALIFLQELQKKVKQIKQKIDDLQEQIKRLEMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XV23</accession>
    <name>A0A8S1XV23_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1400131</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000140">
        <property type="protein sequence ID" value="CAD8205396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XV23"/>
    <dbReference type="OrthoDB" id="10410094at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8205396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="489" mass="57850" checksum="7CCC636042D5E71B" modified="2022-10-12" version="1">MIGSYIRTATFILIGLIKSSIQCDSATLQGCLLDDSIETIKMPLLDYSAYGYGFWFQYLSTQTTLYESLTTFPKIEGLLILREINPDNSQYRVFFYQDWTNKNANQEKYLYLQYQIDNQMIELAKFVFDSFNFDGKWIHIQISYQYPYQRQIVIYDIWNDNCQIINYESNSNLEMKETIITQGGELKLQLNQNDEIKQFAYFPGRISKVQFNFNKDYIIFNTVQQFRLFLDQQFIPQPYCQVNTYDVTSISNYIYSYGLIQLESWIKIDYNLNINYSASVFGVEILFLPHLNSYQKIVDLQYCLLQAWTSDLANGVYFQTFNQIDPDFYPNLHQYFDKSDIAFHTLKQDLRQWHYVSILYGFDEQQVPVSNLKMWFSDGDIYDVQYHNRTYHFDGANLKIRSGQLFSAASDGMYITTESSKITVCLPESFILMKKCHFSCLDCNGPYLDQCISCDQKISEFLSKFYLRIQCKELNSIIIWIFSNQRIQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XVW1</accession>
    <name>A0A8S1XVW1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">OTU domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1410025</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000141">
        <property type="protein sequence ID" value="CAD8205561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XVW1"/>
    <dbReference type="OrthoDB" id="18915at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071108">
        <property type="term" value="P:protein K48-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22749">
        <property type="entry name" value="Otubain_C65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1300.20:FF:000006">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019400">
        <property type="entry name" value="Peptidase_C65_otubain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12931:SF15">
        <property type="entry name" value="UBIQUITIN THIOESTERASE OTUBAIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12931">
        <property type="entry name" value="UBIQUITIN THIOLESTERASE PROTEIN OTUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10275">
        <property type="entry name" value="Peptidase_C65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="domain" description="OTU" evidence="2">
        <location>
            <begin position="160"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8205561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="359" mass="42692" checksum="497B8E9A244352C5" modified="2022-10-12" version="1">MTQLLYYFVGIIVVFLIVFILVKKYCKSNNPENQHYNFELEPIGPKGYQTEIQIQNNNEAHCKINIISDQMSSNRQMSSINSKIQQSIQLNTPSHFKADTQRNKFVQDTINYLYDNSCRKRNVGNNFKYFLGEYYQSSDFLQQYNVKKLVSKQQLKQICTAYRAVRGDGNCFYTAFGFQFLEILLLKYTNDQFYKFLNENQIPFQIKLQNENIIDESNQQAFRNEFFYRLERLKLIQDINIRKQRLFEEFRAYEKENEQIDGCLYGLTTIFFRNLANKAVELNQLINHNFDSQILFQWEVECNDNEIVISSLANFLKLFIQLIFFTNSDFIVNKYGMEDDQKIILLIKPGHYNIGIKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XYK6</accession>
    <name>A0A8S1XYK6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1400079</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000140">
        <property type="protein sequence ID" value="CAD8205292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XYK6"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45735">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45735:SF2">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="6"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8205292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="189" mass="22172" checksum="A258F5D84EE3A4D4" modified="2022-10-12" version="1">MPPKGNQIHVSNIQSLAKNEEVEQLLSSIGPLVEWTPKREGEVKFSCTAEYYDEFTANIAVETLNGYKFQGRELKVKLHTNLSTYKILPYAIEKVNYKTSNTYSMSLPLEEFYTSLTTTKKLEIVIDLKHTFQNREELLEKLLSENPRMTEFILKIQKDVIKEFKANNANNDDISHYNHYQQFQNHQRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y485</accession>
    <name>A0A8S1Y485_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1490142</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lin-54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000149">
        <property type="protein sequence ID" value="CAD8208113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y485"/>
    <dbReference type="OrthoDB" id="6283463at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005172">
        <property type="entry name" value="CRC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028307">
        <property type="entry name" value="Lin-54_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446:SF34">
        <property type="entry name" value="PROTEIN LIN-54 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446">
        <property type="entry name" value="TESMIN/TSO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03638">
        <property type="entry name" value="TCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51634">
        <property type="entry name" value="CRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRC" evidence="4">
        <location>
            <begin position="84"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8208113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="222" mass="26460" checksum="C48DC9A8A12AAEF9" modified="2022-10-12" version="1">MQIQEFLDDLKLPYLQKFYSICSNISEFDQQKQSPKLRKWDSQIEPEQISEGYPIQCETNNIHEENHKVEEIVQDEKKEIQEVELFLCNCQKTGCLKMYCSCFHNGRVCGKQCRCEDCKNREEYKTQRMKAVDNVNKKAHRNKKIPKEKLFETNEIWGCNCSKTRCVKKYCECFIRGKKCTVECNCNHCDNGKDEDLVNEIKKQNEKPNIQKRIRKERQPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y4H6</accession>
    <name>A0A8S1Y4H6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1500010</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000150">
        <property type="protein sequence ID" value="CAD8208193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y4H6"/>
    <dbReference type="OrthoDB" id="6360546at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043014">
        <property type="term" value="F:alpha-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060285">
        <property type="term" value="P:cilium-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.170:FF:000002">
        <property type="entry name" value="EF-hand domain (C-terminal) containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.170:FF:000008">
        <property type="entry name" value="EF-hand domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.170:FF:000017">
        <property type="entry name" value="Protofilament ribbon protein of flagellar microtubules"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006602">
        <property type="entry name" value="DM10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040193">
        <property type="entry name" value="EFHC1/EFHC2/EFHB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12086">
        <property type="entry name" value="EF-HAND DOMAIN C-TERMINAL CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12086:SF9">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06565">
        <property type="entry name" value="DM10_dom"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00676">
        <property type="entry name" value="DM10"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51336">
        <property type="entry name" value="DM10"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="DM10" evidence="8">
        <location>
            <begin position="91"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="DM10" evidence="8">
        <location>
            <begin position="254"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="DM10" evidence="8">
        <location>
            <begin position="422"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="546"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8208193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="617" mass="73271" checksum="B3D79B1440F564FE" modified="2022-10-12" version="1">MSLNSQFPKQVPFLPGHVFSDPFKENHHKTQQFSYINGTVQEKTNFIHEEFDPNLLDSMKFSTPPNLTYGRRQPPENDYIPRIQPPWLKYDRAVLRFYGYFQESVVENPNENYRIRKVIIYYYLSDGTIHITEPRIPNSGIPQGLFIKRQKIPKMLGKQDFYQWEDFQLCQNVNFFERVFRIQDCDSYTREFYEYMGKPLGLPEQLPKDNFEGQRETKELKINPPDTKEYKEYFEVKLGGGHPNGGLNKYLQNDRKVLSFDIIWDDSSIEGQLNYYTLNYYLADDTCEVKEVRKQNSGKDPYPLMLRRQKIPKQPILTHYPGMTLKKEEYYSPQDLICGNTVKIYGRECFIYGCDDFTKEYYLNVLGIQQKPAVLKQERGKKFYHPVPPYNGYGYEEDSLGSVYSLQPKPPKKDVRKNFTQDQFILRFEARLISEVREENSRRFIISFYCGDDTIQVYQTSERNSGIWGGKFLERSRQKNPLTNDYYTEKDFQLGAIVQFNVYKFQLIRGDEFTINYMKQKPDVFKEADISQIIAKLRLFADNYPNFDSFLLDLMKKLDKQLKGYIDFDELSQGLKELGFNLTLQEQYILMREFDKNGEWKLCMRSLWEGLVGRRNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y4J7</accession>
    <name>A0A8S1Y4J7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1510063</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000151">
        <property type="protein sequence ID" value="CAD8208560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y4J7"/>
    <dbReference type="OrthoDB" id="299433at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8208560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="198" mass="23138" checksum="C8969A5310B20011" modified="2022-10-12" version="1">MKTMMSTISNISHNYRASCKSEQSITPLRVPYNGGSQQNCLSKKSCTNTNFIESVIIEYKQLCAWVIREINLKIDYNQMMSNINKQLFQFERFLAKKLDGYTQEIQALKLVHQENQVSIHKLSSTNQDLKNKLKQISNNINEDFLSNSPLKKRHNYSIELITKQPLRNISYSLQEPSCDKENREIFPKKKSLKPKFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y6H1</accession>
    <name>A0A8S1Y6H1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1550075</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000155">
        <property type="protein sequence ID" value="CAD8209615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y6H1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8209615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="515" mass="62490" checksum="9043B1F087DE1390" modified="2022-10-12" version="1">MKEALEDNRFVQYHQTNHQNKSFQTLEAIEFVIQKQEIKDELYKAIIKAQDVTNPANQNSERAKPETVDGDSLQVKEQFCNDDFKNKLQLFSAEQIEEEQIIDYNFTNICKHLLQDQKYEQYLSIFLKFFQNTNLNMEQKEDLLLFVIVIKLNFEKLPQIENNYLEKRIIDYYILLKDRIKKELDIEYQNQFKNSENYENINCYQYQNQVLPNGTIERQKKQRKIDHSLILVDKLDTIKNKDRLNESVDMLLTISLQVKEMFQEEIKDQEKKDYYMNKLQKFSQKKIMKKFEIKQFKDNQYNFISQYLIRQENEESLKILLSCFIEYTFQWINLLDFKQNGKSQKFKQKENSQTFKEIQKFLKQKIIEQTTIKLGIQNYFQNKTYQKFDFIEFVINLLKIYQELKNLEIQEWIIDQQQQEIDLQQLDAYQQQLFHFLKSISEMKLDRYKQKMNINFSLKTFKKLISCDAKPEVLNAIKAAKRKPKQFLNELILNDEEIKDFQEAIEKLRKKAKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8S7</accession>
    <name>A0A8S1Y8S7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1540047</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000154">
        <property type="protein sequence ID" value="CAD8209297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y8S7"/>
    <dbReference type="OrthoDB" id="297737at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8209297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="482" mass="56939" checksum="9FA6CFF2C2DBE302" modified="2022-10-12" version="1">MNENKKNRVFSFQIKNTSGVLSEVLDSQLSTTRFQGIESSHNSLKPNFFFQESEQIQSPRISTQRSLSRLHINSLSPKNSYIYSRKSILTAINSQLGMLYHFEKQYAECLKSNYQIGVIESLFNLMVIAVEQDNIDLLITFMRLCALTFISFQEWTKALLFFQHCKFLSEFTRQLDVIQWTFLQIGILCKYIKKYDIGKIFIKKSLEYSWLIKDLDQEIICYEELGKLCFLNYELQTAKSLHEKSMKGTFEKEQSSLRQVSKKGMEQILNMFPNQEKKLGLHIFSKAVNFPLKFVQCQNQQYFQKRSVKNQYLISNDFECIPNQRIIEIDLTLDDMLQSFLKGVNFSFEIPIPISITEEYESIAEYNSMPSTQKMKQTQLATIIKLHSQKVRTTKNKSPIIRLDQSQQKLPLEQIIKQRLEMKFDTSYTHREKIRNRKFVNNLEQIRLTHEKNSITLSLEHQKRELIRYAQKLLNQQQITFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y969</accession>
    <name>A0A8S1Y969_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1560020</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000156">
        <property type="protein sequence ID" value="CAD8209758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y969"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8209758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="710" mass="83712" checksum="842F1471E1E3A13B" modified="2022-10-12" version="1">MGISCSNYQYILRGIDHQQQGQQNENNHKQITKQVFDCEVWKMLSRRWFQKIIQIITITNENERKYFFDGQLLRIELRNNQSVGFLNYTNLEQLYHLNWSGKYGLFNQKVGRWITTWKGEMLRDAGGWYSDDEKKQGLWIELTKNYRNKAEVYESGYYIDDQRYGIWNYIYNEQLIDAGCYNYQGRKNGKWTELSDQFSYYSQVKYTGEYKNGKKVGLWEVWYQELNTYKYNLIGCGSYDGDGNKIGQWIELSEGYKQFEKITYTGQYKSGKKIGRWDIWFDKGQNKIENTIGGGLYDKNGTKIGKWIEVSDGFQQYPLITYIGQYRNGNKIGRWDIFEHGEMIGGGSYDEVDSIKIGRWIEPSDYFGYDWQTIYNGEYKNGKKVDRWDIEKRDDKGTHFKKIGGGSYDKEQGSIKIGFWCEFDDHNNFDECITTKGEYKNGNKIGRWETLWKSKFDETDKLIGFGSYDELSSNKFGKWIEISDTFQYLSQVTYIGEYINGVKVGRWDIYYQKNSRDTKNELIGGGSYNDEKHGLIKIGKWIELGHSFNISSQVTFIGEYKSGKRVGRWEIWFQQNYGDQKFQQVGGGSYDEEQGYIKVGRWIELTDNFNKKSQVAFKGEYKNCRKIGRWDICCIKYFGDEQNQLVGGGQYSEQQGLIKCGLWIDLCQGFLTSYQCTLTGQYKNSRKVGIWYITFKVEDEVFWHSQLDQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YAA8</accession>
    <name>A0A8S1YAA8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">MIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T1610106</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000161">
        <property type="protein sequence ID" value="CAD8211005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="76898at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23263">
        <property type="entry name" value="beta-trefoil_MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014821">
        <property type="entry name" value="Ins145_P3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013662">
        <property type="entry name" value="RIH_assoc-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000699">
        <property type="entry name" value="RIH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015925">
        <property type="entry name" value="Ryanodine_IP3_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13715:SF99">
        <property type="entry name" value="INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR-LIKE PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13715">
        <property type="entry name" value="RYANODINE RECEPTOR AND IP3 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08709">
        <property type="entry name" value="Ins145_P3_rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08454">
        <property type="entry name" value="RIH_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01365">
        <property type="entry name" value="RYDR_ITPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2314"/>
            <end position="2333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2457"/>
            <end position="2485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2530"/>
            <end position="2551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2572"/>
            <end position="2592"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2598"/>
            <end position="2622"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2643"/>
            <end position="2667"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2721"/>
            <end position="2742"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="12">
        <location>
            <begin position="368"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="2142"/>
            <end position="2165"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="68"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="694"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="2146"/>
            <end position="2165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8211005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2886" mass="340190" checksum="ADD639491338EC8C" modified="2022-10-12" version="1">MSQPLRYGQIISLKPSEMDHMFVISDGHIKTKAQIFEIKTKKVASGTLFFQSLFQIYPQFQTSKIDQALKLERELQKEKYQKDNMKSNKIKEMEEKVFQEYKQNIETAEKNKDQIFTMAQPIQFLHLSSNKFLACNYFEADEEKENFKLELNEYPSDNTCFRLMPAYFHQTRADGLIADEPLYIIMDKIYLGMKPYLHMSESTKRYNNHLILASRTMFNFPNAKALQRKIISGQTKSKQSRIDLNDLQEPNTQIMLMALDYKKKQDLELAHQLASAESIQKKEINVSLEKRSPFLISVFSNTEEDENKIYFGDVIWLHHIELNAILVTVKNMSDEIIVSLQQTKNDQLGEFIGNTNGMWYIESLNMKGGPVEWGQIFRLRHFSLGKYLAVQKEQDRDTDTYSCFYLEDKQSENSLLQFSALPSSINENEKYVTKDGFFKLKNNKKWIQFIDKHPDSDSSIVMLRLSDNCKDDDVLKCYKANLNEIQETSFLVSCFPIMRKTLSILTDLATKDYKKIYQDKNIKFPKKIAQLKKVLQDLTRFCSNQFLISSQNRQKVLKEQYFIDILLEILCNISNEGEIKEYLELTRKRKGHGFSDTLQRSAAQKLHSAAFQVKDSLLQDFGQVASRVQQENDKKQLRQYIGEKFEVIKMIYQLLICICKDNNQNEAYVYDRVDKFQYQAKFFKETTDFIISLLKNNEQLLQNLTEKIRLSEQKQRRIRHESIKSYPQFRLDSSLLQRDPNNIVLFYKKLVEESQNSKDYIEFFRTICKYNNKGLSVNQETIFKKCQENPKFNNALFWTITCTDNQMLIEKDIEKKTMFQLNKEELESFVAQLRLYSDLALSRNFQWKGILEKKFPKQFTFEQIFNNELDPDVRSALCNLALTVYIDHEPLHLQIVPNLCRLVNQQAIKQQQEFHDHEIFKQLIPKVMTQIHDYKKNLIDDLTKGSQVSKKKKSEAQLQLDIIKLAKTLIQFDVVNLINRKDIYSEILQPLITFLEYDKNNYLLSYLMNLQREETLKRAKNSNKIISTMKGMVTGAIDVGRALVNVVVRKKQDLEEEFAEQTLFSKHPITASLLTITSKIQSLNTEKGEETYQIDIEQKMEVCELLIFFQEMRLDFLITNFLAFYAVKQKKKSNKLKYELEFDLLQVLPPTLRTGIEEPTKQGAKNIIQWLGDIISRMGRFLNKKFTNFTKEQEMPDLDTLMSGANKDLVKEAVLPSLLIVFHVSSDCLLLDKVVEVIRRCFSQKLELFEKMRDLEILFDQTEINCYQKMVQLLSRLRTLTESSEVWINTFHNKSSEKETSNESKELHQVQHITKDLDSLLYKHTTVTPNLEIITQDDFLQISTSRQKVFNFLNGHTPLINFLRDSQNQLCDFLKGGYRADYQQTVSELLKTIYQCLTNFCKGNRPNQNLLAQHFELFVDEIEIDFGQTTLLCVIYEDNKFLCENIPDSRIKQFLNYIREFGRQSRFLELLKTIQICKGQSLVENQLKILSFLLPHQKEENKKAHEYLLWGQFTKDSKGIEFNFFNDGQDKEQKHQPYKYHCKLIDLLLQTTQGEESYKLNTPKLKQIFSLGYLVNLVAEENDEFIEGYYPNGTKDANQLKIKVILFINWIYIQNSLTQQEVMIKEQANFNKLFNKEKERLLKLSSYPNRDEYLKYFFDHLVPLFENFVNKIYSQEKAQRDDNYDVLKEILETIAMMSDGILCQYLTKNQYNALYRLYKPFEENTNRLQMNFSSAIKDQNDVLSSNKKSVYEPPPPEFMKSSLKKNQVMTIFNKKEKSFLTEQFKTPNSNDKSKMSRNKFSQTSQQLWVNKPNWFQFLDMCEQSQVLQDKIKEELHTLASAIFHIDKLVSDKMNTNQSNSVNLDFKGFLKKYIRFVEQGIINKAGKKNLIYSLQLLEELLTINDLEEMQNLFDQQNATRMILNIIADYKIYPFDDDFFNQLLSFGCKLLEGGNPKVQKTIFNYFSTYSRSENIFSKLNMVINDHIEELNIKYKIQNEMQKEEKQQQILQLDTEPQVPQDQVNDHTQEMQFKQKLRESLLINVLRFVQLFCEGHNLDLQNYIRQQFNSRNNYNLVSSIIELLYMYHLELTNENYENILRCLDTLTEFVQGPCCQNQQTIIDSKFLEVANSLLATQASKTKSKEHSSKSFFSQDEQSNQKNSSSNLSVGKIKSGKSFKNNRINIKKYMLERIKYKVMVLVTSLLELNSDSLAIKRIMRSLPIEILKKNLTQIYKKHKKLYGPTYTQDALKHIDEDPEANTEKPEFHEAILETGFYIYFLILYYYELDTQEIDQETSTELQKAKNSLFNKNLFMDSLIGQLFTFAFAIIGGVLTTISQAKNYLQKAVERIGQKIDSTEIDKKNQKQKEINQEIFRETIQFFAYNSAHVEVVRNNQIERIQFYKPPYCKYLPKERKKEFHETVNRDSTNSKISDLIEQTDSIIEVCKHEEKLAVFFSKNKFIAIFANYVILWKDLAFILTLLLNLFIILSYADNENTRSQTGDEIESIQTIRRNRISNPILLNSNLTVEETESLFFICGIIMIVCSTFVVLFFLLKKAPLYIKESYKKQDLSEYGVLINSLFQIFNFTYSIARVLLNIEILYYLSYGTLAFLATFYHPFFFAFHLTEIVIRFPQLRNIIKSFWEPKLSLLLTFILIILFNYFFTLFAYIFFYDDYSGKCESLLYCFLETFDKAFKNNGGIGGWLDSNQPQDPGNYNYGRFFFDNLYNIVIVIIMIQIFSGIIIDTFSSLREKQMQRDQDIQETCFICGFSRELFDRKSEAGFKLHTQYEHYMWNYVFYISYLKEKEPTEYTGIESYIAKKLKNYDNSWIPINKAMVLKNMVLESQHYETEKLQDIQREMDYIKKTSFEIYKEMEELNIQFLNKQLLNQEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YAB2</accession>
    <name>A0A8S1YAB2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1600013</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000160">
        <property type="protein sequence ID" value="CAD8210590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YAB2"/>
    <dbReference type="OrthoDB" id="289498at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024395">
        <property type="entry name" value="CLASP_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567">
        <property type="entry name" value="CLASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567:SF87">
        <property type="entry name" value="CRESCERIN-LIKE PROTEIN CHE-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12348">
        <property type="entry name" value="CLASP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TOG" evidence="2">
        <location>
            <begin position="183"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="2">
        <location>
            <begin position="540"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="2">
        <location>
            <begin position="801"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="402"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="455"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="413"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="455"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="504"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8210590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1007" mass="115995" checksum="E757FC94AC1336F9" modified="2022-10-12" version="1">MLNELIQLFCSNGTHSSELLYNATLQLNEFLDKKDVVRCFPRVSENLGDQLPNLRKASHQLFQAYIRVNESFDAVLSAMLSRGLVHPNFLVRQKNVNSLQSLFLSEGQHLEAGSGMVKNMIEMLLGKLCDCNIAVCKATEQTLITLISLNQYKQCHAQLPMGIQSQVEHFVEQMKETNQIRQELRLREPISNGLPFDKLEDLKDTQWQMRAEAIQRIYEELQDVVLTPLQVEVLFEHIVNLVNDHNFNIVLTTLQIMQRCLQFNIVKSSNIVNIYTKLGDSKSAIRTAVRQVLVMYLQKIGDGELLVQILQCPNKNTFYKEESLDLLIDLVQRNKMLLSQYLELAIKEVAPFLEDQKQKLRSKTIETLTQLATINQPLTKRVFQQLGITEDLFNQIDVSTASSNKSDLPQFRFKKEQRDPKEQYAQSFPEPKHQQQFDIPIFQSQQQGYVPTIISQPQKQQRSAQKAESHPQSSSIEFRQAQQEQEYSQPPATQQSKPFRIVKNPQQDIPDQDTGLTYTKPFQRKPKAEADKTFQPIYLDSVQPLDQPESVLKTLLNELRYDDWNRQFEALNNLRRLAKHNTELLRKSNNFPQILQEMVKQIENLRSGVSKNALISLQELSDIYKKDLDCVMDQALQKLIKKAIDLNTFISEEVRKSTISLLQNCSEQKSISLITQVYQSKSIAIKVNICYALNNLLDPNKQHFEKMLQILCLYACDQGQEVRQVAKEGLLSLINQMDKRELESLIIKLAPDGECKRIMTIINGESTTTSEFRKFGATQTKEARTPELRNRTPNTKKLSADFEQMPNYMNQAELGKDWKQKKEAIDWLCNFAKKEAQALNGHKYSNKFLDLLCKLIDDGNKNIALYTCEQFIQLVEPLKLPIQNNYVNIWNGVFKVVSSTNNQVRQSAESLFHELMIHIDIQYSLPQIQHLILYGPAKSKGIAITMLANFVQQFYDERPQLVVKYLVPLAKKLQEEQKPDIKIASQKLFQALVSTCPNDVAHLKLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YE43</accession>
    <name>A0A8S1YE43_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1640004</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000164">
        <property type="protein sequence ID" value="CAD8211488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YE43"/>
    <dbReference type="OrthoDB" id="9992270at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40135">
        <property type="entry name" value="MITOCHONDRIAL PHOSPHATE CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40135:SF1">
        <property type="entry name" value="MITOCHONDRIAL PHOSPHATE CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035754041" evidence="2">
        <location>
            <begin position="26"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="21"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8211488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="67" mass="7884" checksum="12C7AEFA59D621C4" modified="2022-10-12" version="1">MNFAIGFVALCFQGLVLYPWHETISEQVAKLENNIVRLEQMEAQLKTHMERNQELIKQRIIENGKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YEL1</accession>
    <name>A0A8S1YEL1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1720016</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000172">
        <property type="protein sequence ID" value="CAD8212916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YEL1"/>
    <dbReference type="OrthoDB" id="10693404at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="126"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="168"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="126"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8212916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="374" mass="44268" checksum="D924AFA57C805ADD" modified="2022-10-12" version="1">MDYENLLQKNPTFYQQSKTHRSSSANQNSQKQGCPQQIKPKALLKKLEEILQQRQQQFNQVKLKKVNQCKSNYTERISGKKSVQILLDDEKWKADLQNFVTQKKERKSCYPNMNSLQPSAQNLFDQFSQDSNSRNPTITNRSKKGEISPKKGRANSIMDRQNSNDRDKLKLIQLNQELQIQNNHLNEQLRFEQNQNTKLSIQIISMNDQITLLTKYSIFLFRKQLEYRDKENDDIIRSLKSQLDMIKPEYYKQQESIANQERQLIAYQKIEKDMLELEQIIPQISTFTTLFIEQNVQISELQDLLTLQNNIIDKVLNKKDFPLNNLILGREPKKPKQYNKDLPFESSQNLDNLIKLTRQQVNLLTERFIHELFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YGZ9</accession>
    <name>A0A8S1YGZ9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1660033</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000166">
        <property type="protein sequence ID" value="CAD8211997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YGZ9"/>
    <dbReference type="OrthoDB" id="199930at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050754">
        <property type="entry name" value="FKBP4/5/8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46512">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46512:SF9">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8211997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="317" mass="37040" checksum="2CA6988C79B4DF57" modified="2022-10-12" version="1">MNQSSWFSIEDLELEHQTSQVNPPIIKERKLPEKLNMDSILSQYKQIEINSNLIKYIVSESQTTENNKQINVKSKIKLKCEERDLEGNLANKGTNNIQATKISMASSIPYIQGLRSAFLTMKEGDVAWFKLSNEQMYTEQEIASGISVQNLQKYFKIDILEVTQPELPIDITSLENRLKQLELFKIEGNQFYQKQQYQSALLRYQRGLNFLEKWPKKFEDNPVAIEAKRNSLLVLSSNKAQCLIKLQDFKQAISVLEPLIGQMRNKEYEVKNYYRLITCLKEIDEQVKADFYFGQVSHMCQLSEDQKRLFKSIKQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YN16</accession>
    <name>A0A8S1YN16_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T3000001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000300">
        <property type="protein sequence ID" value="CAD8215048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YN16"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8215048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="65" mass="7592" checksum="7688AC2AA4630797" modified="2022-10-12" version="1">MSQSKFQKLIKNVNVNHFMANSFYDEIKCLFALQNVLNNFKVQISERKLLLKKQLQTESQEQASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RYE9</accession>
    <name>A0A8S1RYE9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATP-dependent RNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0020209</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RYE9"/>
    <dbReference type="OrthoDB" id="4310724at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 6" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="11">
        <location>
            <begin position="2"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="33"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="459"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="252"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="621"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="5">
        <location>
            <begin position="2"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="252"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="341"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8133058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="730" mass="85769" checksum="631DD710EFC45696" modified="2022-10-12" version="1">MNWNQFEAIHQEVIQNLENNGFTTPTPVQVEVLNNYQKHKHIIIASQTGSGKTLAFGIPLISEILKNINNYPAKQIIALILTPTRELAMQIYKHLKAITNLSIGCLVGGMSKEKQRRVINAAPLILIATPGRLWDFIENEENEKIKKLNLIKFLIIDEADRMVELGHFPQLDNIMTKITTPSQITHDKERIISMMKEQSEAAYLNNQKITFEVEKPVEMSYEDFIKLQNGKGKIYIDNDIIQNDIEEINKDDEVEQEQNKDEEQEQENEEEEQEEVEEEEEQEEVEEEEQEEQEEEEEQEQDMSDQEDIKEEEIENEDEENIEDEIDVSNDDDEENEKGKKQIKKQQKQKQKKRPQNFQQQDYSLKTRYELRTFLVSATLTHQFKTGSKFQFLKSSKNDQKNQADKLLIKQMKATVPKLQSLLGKLKIKEQPYIVDLTQQLVFPEKLLFFKSIMEEDDKLLHIYHWFKNNEDQTFIIFLNSITYANKVTSMLKVLGLPAVTLHSQQQQRQRLTKLDQFTAKKSNIMVCTDVAARGLDIVGVENVIHYQVPFTADTFVHRCGRTARINKEGKTLVLIGPKDMARFAKLESDLLSSGIKFEDFKLTFNQVKKSREMIKQAQRLEKEDHSIKKKKNMKEALQKQLKNDDFDLDEKELKQELENIEEELIKKRKKLDHEKMQYQQLVSQKNKLDGRFERRRNGLFLSPEDTLQLAEKLKHQKEMLQLKQQNQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RYS7</accession>
    <name>A0A8S1RYS7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">YbaK/aminoacyl-tRNA synthetase-associated domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0010663</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RYS7"/>
    <dbReference type="OrthoDB" id="308744at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04336">
        <property type="entry name" value="YeaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044786">
        <property type="entry name" value="PROXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007214">
        <property type="entry name" value="YbaK/aa-tRNA-synth-assoc-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30411">
        <property type="entry name" value="CYTOPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30411:SF9">
        <property type="entry name" value="MULTIFUNCTIONAL SER_THR-TRNA DEACYLASE PROXP-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04073">
        <property type="entry name" value="tRNA_edit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YbaK/aminoacyl-tRNA synthetase-associated" evidence="1">
        <location>
            <begin position="27"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8132540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="160" mass="18080" checksum="4B0671E295F18591" modified="2022-10-12" version="1">MQENTQTFEAIQQLLNTNNIQFKLLIHEPTKTSEESAKVRGVSLDSGAKAMLLQNKKTQLFILCVMSASKKLSWKLIRKLLDCKQLDLANEQQVYEITKCVTGAVPPFGSIFKVQTYLDPSLITQGDQINFNAGLRTHSVAMSIQDYLKIENPIQLQFCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S002</accession>
    <name>A0A8S1S002_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0020490</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S002"/>
    <dbReference type="OrthoDB" id="291490at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8133608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="543" mass="65179" checksum="143040150D54E9E8" modified="2022-10-12" version="1">MLSRLCAQKFTTQIHGVPIKLLEAMKFDIPKVSEERYKNIRTLTCYLQDRLHDSQLMDFVREVQLIKEKEYTNTLQLKSSIFRLFNQYQVLDLVPLALRIDLQELQPEHFYDDFDRIETEIKSEYTCFVQNRASLFQPQNKSLTDFIQYKRKHHKLKIEQDEKVPAFSQIIDDSLINTLLPKQEMDSYEANSKEIVQNQLVLARNSRLMNKHIKQKDESELRTPLQLKQIDNVEGIKETNQVCLYNLPYILDEKFKQDCKEYFEQRFGEIESIEYFEYCNFKKQIDQIATNKSNENKDFYQSLDQLSSTKKLLERVKVNKNKKLYKSYAIINFKNKESKQNALYQDLRIFGIKFKDLQLRIDDADHKKLLYINNLSKFSNVKYFVEFLKLHLGIQFEPLDERIQLQNNIVCLILKDFQETKEAFDKLNSLTFSRFKMNAFHYPDGLKYMGSRIAEHFNSNTALLILDQNLQKLRFEMDQYWKEVQQEKQEREIVTKESQQPIRQEIHSTDMQQCIIEMIEESYQEEEFQLELFGLVWDEEAQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S0L7</accession>
    <name>A0A8S1S0L7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0010549</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S0L7"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019185">
        <property type="term" value="C:snRNA-activating protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001006">
        <property type="term" value="F:RNA polymerase III type 3 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042796">
        <property type="term" value="P:snRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051575">
        <property type="entry name" value="Myb-like_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46621">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46621:SF1">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="6">
        <location>
            <begin position="5"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="10"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="60"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8132314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="313" mass="37474" checksum="76F5E454C86A9806" modified="2022-10-12" version="1">MKKVQPGRTWTKQEDNQLLQGVEIYGRDWDKIAKVMNIKSKPLLEERYKNLINQKPKPIWELNEDILLLQMVDKLGKDWEMVQKVVKTKDIASCKRRFAKIRDSCLNLEGEDKDLVLLNQYWYKEDEELLLFLYELYNGDWSEIFKRIPERYPKYIQDQFKQKGLANQLHKWNNEKDARLLGYAFYGIPLSNFEQETGFSVPQCEERLKKLLYKISKSLQQPKKTKEQQQFDEIMQFSETKRKPNKSHENNSMNQLDNQQLNYYSEFQAALNQSKSQKQNQIQNRIAQKLELETEYQNDDDFGIVLDEDFVSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S2C4</accession>
    <name>A0A8S1S2C4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0020536</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S2C4"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025663">
        <property type="entry name" value="AKAP_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14469">
        <property type="entry name" value="AKAP28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="176"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8133655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="471" mass="55875" checksum="436D53E1FCC07926" modified="2022-10-12" version="1">MTDLLERSIHLINNFVSQWTKKYLYYTSYIEETTSGHTFMTVFSLPTPSNPIPVATVKVYFYIPDLTDPSEKTMSFRFENDSLIHLVNRTIRISQMEKWIETILARKDRTCRIMFLGTEFEQTRIINKRMDEVLYEKQPEQEKENLVIQPELLEHDQELVNQFTITEEEKIREIKMLTELLYQCFRQIDKDDMGVISYEEGGQLFLLMGLHLGKHQLEDTLNRLDVSRTGILQFKDISSFGIEILHSIYCQNQALKELREKEEESRFEAQWMLSTDLRNVYKKIVEQCKLKDEDEIHSVNQNVFQQILEEQQFFTEDEIKQIMEIIGQKDVEYQTSDKVLEETIFENLVKGLLEAQRSKMEVFLLEHFKRQDKDNTGLIKINELMIALKNCEKIKLSKVQLYSLQSYIKRNDKDSVNYKQEARKIGAIIKKFFNIDLMEQRSRLPQHTKIDAYIPLKTPEQIQEQIKKVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SCD7</accession>
    <name>A0A8S1SCD7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0050163</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SCD7"/>
    <dbReference type="OrthoDB" id="1405469at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0652">Protein synthesis inhibitor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="104"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="309"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8136464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="344" mass="40220" checksum="4E421CA0110156A2" modified="2022-10-12" version="1">MFSPPKVHPFEENYIQKVLMDIERSPLLQYEIPQDLQLFKSQSLLCDDDQQSPFQGARNLPLQRFNSEVFHDFTGCQQQIIGDNNKFLKSRQFRADLSKFSGDFEVIHQIGRGSKGVVYKVLSKVDGLYYAAKKVPLDDNIQTLTLDGQVQFNCCYQHQGFLYIIMEHCEYSLKQRLMQRVYESEIRQIIVDVCEALQNFPQPHLHIHGGNVLFSKQMKYKLSDYGYSNNTIHQAPEKIKSKLSDIYSLGILLMELMLEKELNQISSLILQKFDNLSYYSVSLKQQIKKMVSIIPDHRPDIQQLINFAKVHLDQELNLLQEQNAELQKQIDEIKPRSRRRIQSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SDX9</accession>
    <name>A0A8S1SDX9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0060003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SDX9"/>
    <dbReference type="OrthoDB" id="197906at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8137262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="285" mass="33338" checksum="A5F2D2D59A0D41F7" modified="2022-10-12" version="1">MAFTFGTASYFLNKSSNIEIDFNCYCEKHELQSMVTECTKFFVRKANIQPQIAFQEFVQCYIKGETVLLRPYQQICNSKMNRLVFVINFNKIFESFEDKSGFDYFQLTKLLCSDFPRDIVVSSLSLYANLDEELLFKKTFTLLQFCKALLFELLFENILQELNTKLNNNGIIDSILPILKKYYPNNDEQLYLPNPAAIFECLMKFSNEKNKACLHPILPFTVDMFSTQYLNGGIVEFSSLKQQLFKNTKVDVQLTKLINLIENSNQRIQDCSRLDEDENKKKKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SE87</accession>
    <name>A0A8S1SE87_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0060440</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8138080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SE87"/>
    <dbReference type="OrthoDB" id="316357at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8138080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="204" mass="23977" checksum="0C7E75594160C70B" modified="2022-10-12" version="1">MQQTPQTQMIYPNPQFMQPYMNCVPQYFMMQQLQQMQQMQQIPSMTSMGPLGMPLLPSQPHMQPLPQMVMPFPIQPPIPAQTKTDQNLPVQVKGTKVKLNLKMIKDIPQEEDSVLDYDEIKKQSGDRQFINLKRKRRLLDQQDAPLMQTRRRSEQSQYLEIREMLNVDDKDMTLIMEAYPDTKSLLQELKNGNLKQEIEDLLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SFA4</accession>
    <name>A0A8S1SFA4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0050515</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8137124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SFA4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8137124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="91" mass="10922" checksum="423F93F48BB5DF13" modified="2022-10-12" version="1">MKNQELLIILMKIGMINLENYQVLIKNVELIVQLYILVHMMMQFKFQYLKQLKKKFIKAAWLDNIMGALKGLQYYSIEYPKLGQHIFQPLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJV5</accession>
    <name>A0A8S1SJV5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0070247</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJV5"/>
    <dbReference type="OrthoDB" id="3045089at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:001171">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047126">
        <property type="entry name" value="RNF141-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109">
        <property type="entry name" value="RING FINGER PROTEIN 141-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109:SF5">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="98"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="361"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8138664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="427" mass="51010" checksum="F41C997365F37338" modified="2022-10-12" version="1">MRDDNTNNVNMKIWQLMFLQQISQSYQIIYLSYLIWKYDLLYLMSQYWMLDIFMLTALGLLRIKFKAIVNNLIMDTLIGSVLKLLLLINVKYYQQFQIQYFSYAIMAYYFIRVLVQNIRKISNNQQISQYSFIIQGIKLLFVLQIMLVTMKHTKTITWNWYSVFSISWGLLIISFIVYFVFLLSIGETIMDYVRNRTTKTQLIGGLWFSLYMNSFSILPMWFLLEVSWYEETNYSNTTSTLFLVLIVYNGIILLGLILFFSYIKLYIIEIRFNTTNQTNQTRQKQKFIQIDIPYKIVQISSTYFEIIRYQKNMITVQHSNQSVIHQKTKLKAFEFVGKTEQLQSPVAERELELIKQIEEKCQICYDVEPNIVLLPCQHGGICEECIIKWLEKQKNCYICRQKIEKYLRVAKTHEEGKFTIRDIAMCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SKW0</accession>
    <name>A0A8S1SKW0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0100025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SKW0"/>
    <dbReference type="OrthoDB" id="71437at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="121"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8141265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="355" mass="40944" checksum="DA9CE5AD8BBD87E1" modified="2022-10-12" version="1">MLQHPKQNTNIYELLPEVSIKMTHISYANAINQKKTLIIIGAGRKIKVFEIPYINGQPGQLKEIQKLKEHSNDVVSLKFFNLPQLNDSFISGSSMVDNSIIIWSKLPKQDGSICYGLQQRLKGHVDSVTNLLIHPIREDIIISGSQDTTIKFWSKDHQWACQQTIKDFSRPIKDLAINYEGNKLVSCSDEKFFLIMEPDQQSQWIVKQKIKFPQEIIRICFITNDILTVLQNKGTQLHFFIQNQHQHFTKSLDHLKIAEPGDSCFGLFPPCYSSNTDTLLIKNGNKVNIVKFKFPISKEGVKKMDSQGDWTCKLEQVIEFNDNFLFGSMSQDGEYIVLWDINSFNIQIRKYINQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SKY2</accession>
    <name>A0A8S1SKY2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein SDA1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0070437</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Required for 60S pre-ribosomal subunits export to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SDA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8139034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SKY2"/>
    <dbReference type="OrthoDB" id="2196187at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027312">
        <property type="entry name" value="Sda1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012977">
        <property type="entry name" value="SDA1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12730">
        <property type="entry name" value="HSDA/SDA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12730:SF0">
        <property type="entry name" value="PROTEIN SDA1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08158">
        <property type="entry name" value="SDA1_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SDA1 N-terminal" evidence="4">
        <location>
            <begin position="78"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="545"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="545"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="559"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="588"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="613"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="651"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="719"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="740"/>
            <end position="759"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8139034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="759" mass="90490" checksum="1C7B52103F72944B" modified="2022-10-12" version="1">MNQQENVIQSISTKSKEYKEECRQLLERIVALQLKVFKAPDLYVKEVLSVVELYKQQFELVKQNPGQNNGKFSIIAQFLSKVAIYYKTDLKFYIEDLMELINHFQNTLNQGLRKKIIFCLLRLRGKNLIEPYRMIVFLMKLFNCQDKDLRRIIFRFIIKDIKQFNKKHRNETLNKQIQNFIIDLIKKSQENIAKRALQIMIELYKKNIWKDNKCVNIIAQGCFNQNYKIKLLACYFLIQTTETHVEIESSEDETDKYIQKKGKNKCVKPTKAREHRVEREKKMADKKQRKKLSKLNSSGGFFALDQIYSPQDFAERLFDNLKKHDSERFSVKLAMMHLISRLIHRHKLLIIPFYTFIQKHLYPNSKDVGKLFAYLAEAIHDQIPKEDLQPTIRHLIDQFVNDRCQELTLTMGLRGIYEILQKHPKILDKDNITYLIHYIKYKNRNVSQAARSILNHFKDTNPQLLDKRLRGNKWKSTEELDGDFDDNFIQQITIGIDGAELLQQEEEERLNKKFDTPIYCDRILTDEDFKRIRALKRKKEAEIEQNKLQEKQQKHQYIEQEEDNDEDFIEDEDDNVDDDISEISGDSAELDDENEEWEDESIEDEDIDDDDIKPDSKITKEQNKKRRQSLSDLEEDSVQEYYSDESQMSDENPHGYVREEDLMRYRKTKSEQKHEKLEELKNGTKEKWYQGPNKKKSEFASTSNKEKQKNKPLQMMRAKRVEQKNKESRIKSKIKDLKNKLGHVRSGKQAQRLKKQKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SPM7</accession>
    <name>A0A8S1SPM7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0100297</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SPM7"/>
    <dbReference type="OrthoDB" id="287461at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8141768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="186" mass="21424" checksum="3012484D825F4E84" modified="2022-10-12" version="1">MQFSLSDKDCQSELPQGLKNANFRKRKPIDSDTKTNLILSVVKDHLPIYQAAIIHKVKYSSAKHILRNYYSDTANYFSLQKKRRRKIQCCGVSALIEINSGNIALNSQGFSTIPFIKNGVNAQIKQKVIDHLGVSIYKQIGNLNFKRLNKKVRIDPIKQLNNIEKIENKVKFLIQTLEKQHQEMIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SPX9</accession>
    <name>A0A8S1SPX9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0090128</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SPX9"/>
    <dbReference type="OrthoDB" id="286637at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02205">
        <property type="entry name" value="CBS_pair_SF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF35">
        <property type="entry name" value="LD22662P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="248"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8140544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="384" mass="44859" checksum="4DB73F48E6DF88DE" modified="2022-10-12" version="1">MNQLQISTQEISKYKDTITQFLKDNTLYDCLAHHNQLIVLDQTFTCWEVFQIFVEENLEETLFWNSDISNYDGVFSHSDLIKVLLKLYENAYNNKSSLQQSGPLFNVIEEESEDQEESIQKIQSQKQQPSELNDDQKLRLIYEMKTISIRQWNRILKDDLESSIGFVQGRNDERLYDACVKIVKSGTSRLFVVDPETLMFQGVIHQKDILSFLIKGFTQYLGAILKQQNNILSQHQISIKVFFSTYNRSKEIITCKENETVYQVFYTMMHNHKSYVIPIVNNNDQYLGQINRRDIILIIKNGLFELFSQTASQLLVFLSNEKARIPAYLYYSKQFFSMDQTIKEVVENLVLSDSSALICLAENKQIQAIITISDILRFILKDAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SQ42</accession>
    <name>A0A8S1SQ42_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0100185</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SQ42"/>
    <dbReference type="OrthoDB" id="313274at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051637">
        <property type="entry name" value="Ank_repeat_dom-contain_49"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24180">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR 2C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24180:SF45">
        <property type="entry name" value="POLY [ADP-RIBOSE] POLYMERASE TANKYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="340"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="121"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8141546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="421" mass="49669" checksum="1685A08EA5AA438C" modified="2022-10-12" version="1">MRRVKTEQSTLIELIKKKEPQTIQLQQKPKNRKISQLGYFNSLNVSNAKVKLSILMRDILIPTQSGDKNLQYKIKNKNRSIIAEDKKPIRNNNKPSRFIDIQENQIFKKQYYTSPKQVFENITLRNNISNQRANVQKIKKNLKLQQQTFEQQQIIHDLIVLPSVINPEQQSKSVAYIKSVRQYEKQEQLYHIEGSLEKTIEDFNIRMFGSRTVKKLINSVKPWVNQLQQKKQSKYKRIRLEMVQLLTHIHKLKLTLEEFSKGDVFPKVPYQSQRSEKFFSFCKMNKGDQIQQLLELEKYLVYEYDPSFMTALHWCCVRNCKTAALILLKNGADPDAQDMIGRTPLFLALLHNNNDIAQLLLYHKADPWNKGILDYNEATTNNFEGKTILQQARRTHILLRMTPPAERQFIWQSKQFLMLCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SSP6</accession>
    <name>A0A8S1SSP6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0100173</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SSP6"/>
    <dbReference type="OrthoDB" id="288932at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="598"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="637"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="699"/>
            <end position="722"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8141524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="872" mass="101160" checksum="D255F73D56CDC69A" modified="2022-10-12" version="1">MINEFLNITYQWCGDQHKIIQGYVLCDDHISECKKYTQQFNCPATFSDENFKIMAAKLDNYKLVIIFSNKFLIDPSKCSFLISTNNKNVPYYPKQMSYGFDPQENYKITLINNQNKYNLREQIFINTFERQVTTGSIIPILTCPKQLLSNIWEYFIEQDCYIDTLEELQEALMMSQRQVQLGNAIIANTLINGCALFQKYIQIKPKFSKKEIENDIMNLTFTDLENSISQFKGMPIMISQNGRFVQQLMILVKNMISQFFNFIEETVDLLLENDFDPQELSYVLEGQIENQVGKFTIQMHEFALVEQADFIILFEKIKETIFDIHKDIEQFIVSTSTFVNEQMQKVNQIFQDYPALANVRTFQAIQTDASQHIVRELQNYFLIEKSNFWRPEFINHFEFTKKIIISYLEYKEEKINNPAKTTDFFTSLIRMGLDPKMPIQTMQIIVDKNDRDFVKEKDELFIEKINIITRGKRSAFKIYLHSKEQLVLYMIKHQIIIVSFDYFNQSQWTEIEKFSNLNMKLSQGVQHILETNIEYVKNIKLYEQDVCGYQIDSNGQSKLIVNFSMNMDEQLKIIKKQFCKKLDKAQKGGTAQPTDPTMLAAGAIGSGIGIFIVDCLDDNISWQKKLQRAGYGTAETTAFAALSMNLPFVGFLVGQTFLFYSVHKVFSNKVLSMQNKLKNMGHLGVKTSMGIGSAVAGQILIPVPVLGALIGSVVGGVGVGLYHKFIVPSTRNSLLGIINRLEQFIQPNGQLKYEKSVIKMMKINPTHFFENQPQTLDCSDWLTLVSINLVNEVAYLYEMQWEEQRKKLFEKEDNAHKIEQIINLENEEDKLVEKLCKWEICRHYIYKENISTFKYAKQVGIFVAGMISNFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SY92</accession>
    <name>A0A8S1SY92_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0120216</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SY92"/>
    <dbReference type="OrthoDB" id="305389at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8143412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="170" mass="20345" checksum="BF992F597D5BB906" modified="2022-10-12" version="1">MNKTKQKQNSLFDLSVIIEKIKNLDEYQSTMFLINKRPNYELLNQSNTQFIPHIINDFEIKRKFKEIQRDSLLSQKPFMIPKTLNKSQIYSKQINTTPNPQEFSIEKKIPSTSFKVQKNIVNKRTKSTDYRIQLKKTVTKDIEAQFLETRNLIKRFNKIKRKSVRFNIQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SZ09</accession>
    <name>A0A8S1SZ09_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0120382</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SZ09"/>
    <dbReference type="OrthoDB" id="10255969at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03263">
        <property type="entry name" value="ABC_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001253">
        <property type="entry name" value="ATP-binding cassette protein subfamily A, member 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001574">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF36">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY A MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="665"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="730"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="756"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="793"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="823"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="850"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="306"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="893"/>
            <end position="1113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8143742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1230" mass="141717" checksum="E0FC83008794455E" modified="2022-10-12" version="1">MNQLKTLLRKNFTLHFRTKQFWFNLYIPILSAIISILVAYKDNVTCYFLLLFICLNNNGVLRQSLVLIVREKSDQFKLLQQQMGLSSNAYYASWIITFFVLTICVSLIYLIPFIVFRLNKVGYGYLRIEMLILGQILYCCAIASLALFLSSFSKNYNKASELIGLLNVILTFVVISNFAPYPKLTFLGQLPTLFIPQTAFQMLLLSSNWLTNNDQMFLNPGIYIICLIFQIIGYTLGFMYFDQVLSGSKKLFFLCNYCKQNTQIYTDFEDELNQSPNNQDVEPYQLYRPMLNIQDQQVENQGQPFIQVENMWKKIDNQYILKSLNMEIYQNEIYCLLGPNGAGKSTLMNVLSGLSDRSKGKINILGQSIDNKQFKNQIGICLQKDILYPELNVYEHLKFYGQLRGILDQELQQQINQILEICLLTNESNIYSYQLSGGNKRKLCLAISLIGNSKIVFLDEPSSGMDALTRQQIWDVIKQMRLNRAIIMTTHHMEEADELATRVGILMNGQIVTQGTPDFIKLNFGVGYHLQMDFQDQNQLLLEKPRIQVQLQKIDSYTSVIQCAPTSLKSVLPVNKIHLFHELLNYLEQNSQSKFSLQLNSLEDSYLAIDYQYGTQNIDLDVQRVFQQRPKIDFKAQLIGLIQRKLLVLLSSSDKQMRYVFPLPLIYLAVILGMQHSFLFVFFYLGIKALTSTLYVMIQIEDKDTKIKQYMYASGVQIHTYWLSNVLTDIIPSFIESLFTTILLLIYDVGHFGQHFFAVFFLITIFGVSMSCFANWVSILYKNKQGVYVNSPLLQFFVFFLLFFIIMLFIIQPQSPIGVNMIAMLLMIISPFAACYMGFAMSPILLNLAIFSYFSCILYLIIGGTIYFLILYSIDKNEYKQIQANMDDTDAVIDVQELTKKQGELETVHKLSFQIKSKEIFGLLGPNGAGKSTTFNMISKFDQPTTGQVRIRDIKTNLGLVPQFCPFYPTLTIYQHLIIYGTLKGQENLQEAIEKYLKALDMYEIKNRQVKFLSGGERRKVQLGIALIGGSNRLFMDEPTTGLDPKSRRIIQQILLQSTINNDASVLLTTHSLQEAQSICNKIGIMVNGYMITQGTLNELRAQLGEQARFSVKCNEGRKEQVHQTICLQLSQQFQLQPIFEYRDNYLSYQIPSGYFKLSNLFQYLQVELKQKQLLITDFSIYQPNLESIFAFYASQQIIGVNQNLFINNGDQFNFWNTLRTITAFFCVLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SZN5</accession>
    <name>A0A8S1SZN5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0130355</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SZN5"/>
    <dbReference type="OrthoDB" id="10250504at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8144697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="205" mass="23815" checksum="147FB5CA7B1355F7" modified="2022-10-12" version="1">MDNLQLTSFKEQTTIVLMPYHLKNISKSIQNLIPKMIFTCRIHNSIPSIVIAVKKVNIVSEYLETFDDQPQMFIDIEYTVTLLQLHSGSLVKGRLVQQFKSHMIVQIFNIFNVLIPEDEINEKSFIWNENFNAFMNKQNEILENHIELQIKITEVILNEADQKEFQIKGSLKERKQTGQIEYLENSIGDQTKIEGIIAKSLDCIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T0L6</accession>
    <name>A0A8S1T0L6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome b-c1 complex subunit Rieske, mitochondrial</fullName>
            <ecNumber evidence="4">7.1.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0140033</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a quinol + 2 Fe(III)-[cytochrome c](out) = a quinone + 2 Fe(II)-[cytochrome c](out) + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:11484"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="7.1.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="4">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="4">The Rieske protein is a high potential 2Fe-2S protein.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.8" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T0L6"/>
    <dbReference type="OrthoDB" id="297702at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008121">
        <property type="term" value="F:quinol-cytochrome-c reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03470">
        <property type="entry name" value="Rieske_cytochrome_bc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014349">
        <property type="entry name" value="Rieske_Fe-S_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006317">
        <property type="entry name" value="Ubiquinol_cyt_c_Rdtase_Fe-S-su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01416">
        <property type="entry name" value="Rieske_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10134">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT RIESKE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Rieske" evidence="6">
        <location>
            <begin position="151"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8145017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="260" mass="29812" checksum="14701EB013FBA15E" modified="2022-10-12" version="1">MISGAFRRSFIGRAHFGLVSEYNSRVNQKLYKGVQVSEAPQFFTTSARPGNFGDHIDFKVQMDNWFDENRVHNEHETEIKRTQIYALNAVYYGGLLSFARLFAVGLIGRLNGWKRYDRDTYLEMDIGDLPPGEVMQIVWNGTPVFIRRLTQQEIKDEDNLPKETILDPNSEVVLTNCGNTKVLVVSALCTHLGCIPIPYLGAYNGWVCICHGSVYDKYARVRQGPALQNLPFINNSIYDDVIVCIEEMKFPREPSQRYWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T4E1</accession>
    <name>A0A8S1T4E1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0160304</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T4E1"/>
    <dbReference type="OrthoDB" id="293148at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001236">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="67"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8147290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="353" mass="40783" checksum="5FECFE1B349323FB" modified="2022-10-12" version="1">MICSYYLCNKLISLDSLMNGLTIQCLWCPNAFYCCEICRKQDSKHQQLCGGSQQEVLVDKSNIIKEMQLLEKIGQGSFGTVHKISIQGQLYALKKMNKNQCSKEVRIHKHLNHKNIIQFHYFIEDEDYIYIIMEYASEGTLTQNRENCNIQSIFIQLCQAIMYLHQKGIIHRDLKPDNILIDKNGNPKICDFGLATYESVISSFSGTFEFMAPEVIKNLPQTQKIDVWSLAAILYWLLENKPLVQGNQLEKMQQILDFTSPKFTKITNPLIKDLLNQMLLPDSDNRISLQGVLNHKWIQSINTIEKEINDISTQPSVQRQQQIEIQQQMQKQKISILQKVVSLFGCGGREKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T6K4</accession>
    <name>A0A8S1T6K4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">succinate dehydrogenase</fullName>
            <ecNumber evidence="5">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPENT_87.1.T0180295</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a quinone + succinate = fumarate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:40523"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate metabolism; tricarboxylic acid cycle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T6K4"/>
    <dbReference type="OrthoDB" id="316616at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.700.10:FF:000001">
        <property type="entry name" value="Mitochondrial succinate dehydrogenase flavoprotein subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.80.40:FF:000002">
        <property type="entry name" value="Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.100:FF:000001">
        <property type="entry name" value="Succinate dehydrogenase flavoprotein subunit (SdhA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE 2 FLAVOPROTEIN SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632:SF51">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9" id="KW-0274">FAD</keyword>
    <keyword evidence="8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="FAD-dependent oxidoreductase 2 FAD-binding" evidence="15">
        <location>
            <begin position="9"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarate reductase/succinate dehydrogenase flavoprotein-like C-terminal" evidence="16">
        <location>
            <begin position="186"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR630664-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8148895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="322" mass="35918" checksum="2342D682B28C5D46" modified="2022-10-12" version="1">MERYAPTAKDLASRDVVSRAMTKEILEGRGVGPEKDHIYLHLNHLPAELLHERLPGISEAAKIFAGVDVTKEPAPVLPTVHYNMGGVPTNYKTEVLNQVNGKDQIVPGLLAAGEAACASVHGANRLGANSLLDIVVFGRQAANLVGEKWKPGQKQPDLPKNAGEAAIARIDRLRHHEGSQTIAQVRKDLQRTMQKHAAVFRIEKTLQEGVEKVKEIYSRKDDVRIKDKGLVWNSDLIEGLELENLLLQGKMTIEGALNRKESRGAHARDDFPDRDDKNWMKHTLARIQDTKSGDVQLTYRDVITKTMDPNEFDTVPPKKRVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T7G7</accession>
    <name>A0A8S1T7G7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0160322</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T7G7"/>
    <dbReference type="OrthoDB" id="10437360at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8147326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="281" mass="33240" checksum="EAD4F08CF679A0FA" modified="2022-10-12" version="1">MLTPYFINESFIDQLFNQNPVSLLITRIQPGRVGFGNGIIKGENFNFQIRRLISFTYQNQKKNYYQLSFSKQFTRFSMFFQNPKSQESYYFAGFRVFPHNSFSAFIGNTKNKGYLVTKQYLDVKILNNYCLSYFLDALKISQNFLSQATIRIFYPNFAESDIRLRTVWKDRYLINMKYDKQPQIEMGLALPLSNSLYFSVISNIFFNDQRINQITQFTQHYKGFITKMGIESNFVNRISTSPYIQFEMVLNQDPCIILKGQYKNQKPSLQINFECDSLKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T8V2</accession>
    <name>A0A8S1T8V2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tubulin-tyrosine ligase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0170151</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T8V2"/>
    <dbReference type="OrthoDB" id="10255472at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070736">
        <property type="term" value="F:protein-glycine ligase activity, initiating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051437">
        <property type="entry name" value="TTLL_monoglycylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45870">
        <property type="entry name" value="TUBULIN MONOGLYCYLASE TTLL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45870:SF2">
        <property type="entry name" value="TUBULIN MONOGLYCYLASE TTLL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8147806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="723" mass="86120" checksum="77B4C05427A02676" modified="2022-10-12" version="1">MLDNRTSYFNVQGNKASFKQNIKLFQSLKSKLQQAKSKSKEPIKGQLKILKRLEDTINEPQMHQRALTTQKRLAAQFEEQDQRNEEINPMRYNLMQLLVLMGKKEYYFDKLAKDDFKLIRVINHQKENVDDHIQKIKKFLSNKVRYKYRSCFKMQFQIQLELLKYVPKKKSMAIKNSQKDYEIDEDEDNSKKKKISARGYQDFSNWKKKNNVAQNQKVFIITNGYHALRDSLLDRNWAENEDAYSPFFDLKWTCGLRSDDFLNLMDFQQINHFDSNLCLCSKFGLARNIRCISNWESFFPRCYDLGDLVDFSDFIEDFKISKIHSILMEFDQNYICNQNKKVITEPYEDSEHKNVLRLKDFKNNTIRMIEDNKNQSFIMNYRDRFKIRLCIMILNKVVCSLSSMIKNILKDEFPFVNPGEWLQLIKDPEKPKDVLIQDQIEMILKVNGYEDQEGDKFIQKYGSQIKDLLKQLNKNPQNSLQGTRNIWIVKPEYSSRGRGIKCLDDLNQILDSVNKETMNYVAMKYIENPLIIKNRKFDIRQWVLVTELVPLKIYFYNECYIRFSAEEFDINQFHNKFAHLTNNAIAKYSQKFYESDIKGNMWTQEEFQQYLLEEFGWDIFGEKIQPKFKEIVINSLKCCCDQMKNRKRSFEVYGYDFMIDEQFNTWLIEVNMSPSSDTTTPVTAQIIPKMLEDIMKVVIDNQNKTKKKVGGFQLIYNSDTKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TAK0</accession>
    <name>A0A8S1TAK0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0170066</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TAK0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8147642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="352" mass="41117" checksum="5914AFFF00338E0C" modified="2022-10-12" version="1">MNIYLSISINLLIGSDSIISLSLTNSNTNYILAGIRCSIALLCVICHLLIYKKFKPSVFTLCQQLIHQIQMSLEIRQAFSSLDSYRFYIYLLLLYQEQSNQQHKYLQYTSIVYCMIQIQINNNFIHQYILTTLILILIILIKLLSLKQINHKQATSHEVLHSYITVPAEQIMTNRKIDLMQKDELICITNQIKQGIIYLSQSLDLLYINHKASKILTAQTDDQAMLQFQKFILQGLEQEESLTKQHKEIQCLTSQKQLPQKADSKMIESIIASAEQQFKRYSSLSEKLLEQQFKYKKQKTNLLSKHDFKQLLLSLFNESQISSLQVFLLLQFRTQEFCQSQQINSSFQYQQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TAX6</accession>
    <name>A0A8S1TAX6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0180225</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="298369at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332:SF175">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035930058" description="EGF-like domain-containing protein" evidence="5">
        <location>
            <begin position="17"/>
            <end position="1682"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1241"/>
            <end position="1266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1333"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1408"/>
            <end position="1428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1440"/>
            <end position="1463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1495"/>
            <end position="1516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1522"/>
            <end position="1541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1553"/>
            <end position="1571"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1586"/>
            <end position="1604"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="155"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="631"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="746"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="793"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="842"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="883"/>
            <end position="934"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8148757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1682" mass="195402" checksum="F6DFFA73F2EC2EDE" modified="2022-10-12" version="1">MIVILITLSLMNRVYSEQTFEKLTYYLDTTTLAQNLTCQSLTKVYQSTTTGTPRVMQFKINTAYGYNSLLYTFDLHTPFTSITDIIFVESGQTVYKLQYNSVRSYVSNFCSITSSIQYVQFTSNTQMNEQQVTLLVSTNSAITFQIKNLNIYAERCQLYCKICNNDKFCSECMPDFILTSMGKCVCNSQFLTYFQGQCVTQCPVNHIFNGLTCLQYNQIINLLYDDTASKFIMYPDYNTTQRECKITLEGKKVAGLFIQNEVIEYQINTPITKAYDIIMETEIYLLNQPYNIPLHLFIKFNNNLIGQALILYNYQNKAQVIGAVNQKQCNIIGFNSCLKFTFILNVQNISDLQQIQINLNIPLEIKNAAWAISYIKVNAIELTPIICPLKRFKNECVDECPIISRAQGNECISIIDDYKFSKILSILNTDRFALKQNLDINNICEYLQKPILPSCQFYQKRYLQGGELIWKNTQIKLDISSIQPHYKLKLFFKAILIDPVDSQQSLIVSIDDTKFILNKNSPNSYCFSSVATIGCIIQEDLGQTNADYLINFEQEFDHSDTQLEILFTCNIKQNINSYCAIYDIIVLQANCPENCKFCQSDSICLQPDPYLEVFYDCPSEGYYYSEGACLKCKLSCKTCNNGNYCITCKDQYFKYGNLCICQMNMVEAFSTDCTNDDCHPSCSKCQKKLNKNQNNQLQSITQLCAFCDKNEHKVFNNNKCECFLGYYMDQQNYPNTCQLCNQTCQTCSDAITCITCFPEQNRIPQNYQCQCKQGYFENGFDTTCIKCISICKSCLYKEEYCTKCYTEQYRQLSKENTCICENGFYEDNKTDVCLKCSDYCNTCSDYSICTSCSDFQYRILDLHTKQCICQQGYYDNEQLNCLPCHYSCSKCNNSNLISQCTACPNSRQKSAHNIDTFECKCRKGYFDDGYLECLSCNNLINPPITHYCYSQCGDLIIQWNEECDDGNLDPRDGCNQCFLQNNNCIDNICLICQHNQCLQCKDGYYLNNDYACSQCSDECLKCELQQNNCTQCKFNTLSNEKCLNCSQEQGFIQIDNQCFSVCGDGIRTYQEYCDDGNQLNGDGCDQYCNVEEGYVCNLECQKINYIQILLQEYKFDSIYNSKRTIQLKLNQEVKISVINTIKDFFNVTSSTPNLKLTIISINDYSSMKNDYFNITLDFNIELDSSAQPPLITITILNSTFFTNQQGYSFKTNNTTIQLIDFIKQDQFVIQNSQNLAQMSSYFLYILLGLAIIAMIFGGLDIFWNLLDTMQLICYLKYFNITYPYNLQYYFTIFGFAEFDFIKSYFDFEYLITQYVDTPEADPKFNLEGYSTVFLINIISVLIVFLTTSATFIIIRVILYFIHKVTKEFTENMILIEREKINIFTFLFYKLANNCQKYFLKIISEFKSALIRTFMASAYDLNIAIFLQFKDIQFQNPILRLSSIFAIIMFFIETYFIYNCFLLMSKDQVTYKLQQTQQNYGSLFEGLRLERNRFNYYFNLFIVIKKALFIALLVFLYNTPCLQISLVSLLSLTQVLYLLLNRSLEDQNELIKQILSELILWLSEILILSFGFNEQSNIFNYSQMTNIGWIIIGFLSLIIFVQLIIDCKQHFQFLDEKYQLLKNLRVWYQQYFQQDEGQSSLNTEFSQFSIIFHKKQKQLPNIPQAQGSDKQINKRRIVSFQLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TC24</accession>
    <name>A0A8S1TC24_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DDT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0180362</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8149014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TC24"/>
    <dbReference type="OrthoDB" id="298907at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018501">
        <property type="entry name" value="DDT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02791">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDT" evidence="4">
        <location>
            <begin position="5"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="171"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8149014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="483" mass="57688" checksum="76D26BED1F2D62E1" modified="2022-10-12" version="1">MSLVNTLQIYEFCRTFASQLNLRTRYTVEQLHQSFESNENSSLAYQLVIGLIDKFCLTYLVQASTQTNSAFLNVIGQIKNYFIRRSILMNIWPTIFTLITRIYRIRAYIKDEIELEYQQLKEHLQQEQFKNWFALDKQKLILFIIDTIFYESEFLIQEIDQRIKIQEILSNNDWRKELAKKETELKRLQQSVGKVAKKKQKEQVKTLQLIAGLEEEIKLYTKASTVSNKQLFHNQAIEKISNNLYVFEEEKRCIYQENNEEDWEVIQGAQMIELIGQQQNKCDQMKNLVNEGYFNLEDCKLEPIALDNTYGIEQISQDSMAIELFQNCDINIVRQILLALEKDYSEYELNYWKSCWTSAEKRKIWQEKVQNADMIELTEILSKIPHQLSWVNKKHGQTDSQAKYKSSRLYWFYQNKLYSMPYQQLSKQEPSLHSMYLLAITLYDKLKSYIHRKMRTMDINQIQQQQQQSDLESTLDDDFQELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TD48</accession>
    <name>A0A8S1TD48_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">P-loop containing nucleoside triphosphate hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0190112</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="392140at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031380">
        <property type="term" value="C:nuclear RNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031048">
        <property type="term" value="P:regulatory ncRNA-mediated heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000326">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:005858">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057373">
        <property type="entry name" value="ZNFX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF341">
        <property type="entry name" value="NFX1-TYPE ZINC FINGER-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25396">
        <property type="entry name" value="ZNFX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ZNFX1" evidence="9">
        <location>
            <begin position="328"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="7">
        <location>
            <begin position="516"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="7">
        <location>
            <begin position="789"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="8">
        <location>
            <begin position="937"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="873"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="27"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8149444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1965" mass="230476" checksum="1B514C74C3A063A1" modified="2022-10-12" version="1">MQNNRNQNNQNNQNYQGKNNKKRSKGQQQQHQQQNQYQSSKQSTSQQQYQQQPVQQLQRNTKGREFRRIKQERIERANQRKLEKQNQEQFFTWFSSISNNTPTIHQIVSYFQDLGNLDNLENIFSNLRIHANQCESYMILMRILGESPYQEYTDLILRSQSFNTQITEKCTLLLIYQNSIQYINQELEIMYKLFSNLKLNDYTTLKQSSISSLYNLMKIIDLYNPQLQQQASKTLTNFDHFLKKIYSTAKLLEWKQTPNYPIALEFFSYNKTPNQLFGKLEILPKENITFERYIDYHYNLIREDYILELRDAIISLNESGFECIDKEKTNNIGLYQNIELLSVGMNNFDIIWRIQVEQFNMDGRKQKHINWAKSKKLASGTLICLTNIECYPLIFGIITKRDEKQFQYKNGKIQLEFRFLDQNTSVMQLLNLFSQKTILMQYEGLIDPILYYLEALKQKVSLPFQKMIIENKNEVNYPSYLNKNYHFDLDLSYNNKYQGIKFNIFQQPWPQFNTSMDQSQVSAIKLMLQKEVALIQGPPGTGKTYCGALAVRLLYENLQKNDFPILIVCYTNHALDQFLEHILKFVPIEQVARLGGASKNPLLEQCQIKSRQKVEFEFRDFNKLKKEISTIIQRMLNYDYKIRPQDIEKYWPELYNSIISNFFQDNDLDQDYYIEENGDELLVKYWINQQKPEENVIVKGYLNGMHKYFKMEQFYKKPQEFVNLQKTPGLIQVQHDSSDDDLDEYLDSEDEAIYHQNGQREFKFDSLGIDQAIKHFNETHQNQNLLHYQYQTIENIKGYLNMQKSNPWKLNEIDIKEILIYLECLKYQNDSIKFAKLYKQFIEQSNILKDLYDENEVQILNSMKIVGVTVTGVAKQTNKLQKLNSKIMVIEEAAEVLESHVASILTSNLQHLILIGDHYQLKPSIKNYYLQEKFKANVSLFERLFLNKIPSVTLTQQRRMKTKFADFIRLIYGESYQDHPDIQKQNQIEIQGLEKDLVFFNHKWLEAQDEKSKENKQEAQMIFQMIQYLLKCGHQQTSISVLSLYLKQAQALKRQFQNAGQHKIKVQTVDNYQGEENDIVIISLVRNNNYNNLGYIKIDNRINVALSRARLGLYVFGNFDFIFNASTPGSTWQKMILLAKSKNCLNDYINTKCIPHGNLQTIKQPGDWSKIKPKLCNKKCLKNYSNCNHLCNKDCHISDIHPADSCPFQCDRIIDCGHKCKLQCRDRCKCNEMIDLNLPCNHKVRALCGTDPLSQKCQEKQEIQFSKCTHITKFICYEREKYLYKCQQICQKKLLCGHICNKICSVDCGICVQECIKVRPCGHQNKCKNLCFQDCSPCDTPVENKLKCGHKTKFPCFSQKQDILTYNCQEPCIKKRQCGHDEQICQNKCHEQCSPCQKLVSRKLKCGHEIDLRCHDADLIVQNFKCLSKCQKPRQCGHNYVCNNQCHQKCSPCLQKIVKKLTCGHQIEIECCKVNEVQQCKQECIKPRVCKHVQQCKNLCFEQCSPCEQVIEHTFQCSHKHNFYCHSYKKSINNFNCNEPCLLKRSCGHTSPCLSKCYQKKCSPCLELIKKKLDCGHIIQLKCYEQNEKQKCHEPCNRIRNCGHIFPCKRLCFETCEPCQQDVLKPLDCGHQQLIKCNIKSQNIICKQDCIKSRQCGHKYQCTNLCYQECTPCKQILQQKLNCGHMIEVECCQSQKIQLNQQCQQQCIRRRDCGHDFPCFNMCFKKDDCTPCQFKTYQTLDCGHQFLILCYQKAQKIKVLCDQSCNKKRPCGHNTICNNKCYEPCSLCSGQCEITLQCGHKRMVNCYQKNDLSFLKELKCMFPCNKIQPCGHNQICTKKCYQQCDPCQVEVKLPLKCGHLQKAICYQNPDTIECLQTCKLTLNCGHPCKSKCGKVCLKCEEPILKIFPQCQHQKLIKCYENQQQLVCGQQIPFIQSCGKHTIQVPCFSRSTLNPKCQKCNDCIIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TDU1</accession>
    <name>A0A8S1TDU1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0200307</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TDU1"/>
    <dbReference type="OrthoDB" id="300659at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8150530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="237" mass="28251" checksum="2F1F389D15613831" modified="2022-10-12" version="1">MQNRNIIIFSPNQQELQENQQSIENHTYQAQSINAIQYQYQNMQQPQIQRIKLEEGVQKQPLIKIRNDIQINLERQGYFQKIFQSVSDDINANNQQYNLYPYIKDDQVIFQFWKFEFKGQGNFQDRYISGILKLKPDHPLSAPIFYFDPIHTDQGDEVIQNESVYGDHSLCIPLFSYWKKTTKEQEILQAIEQNFNNPSWSQSDVPANPLFAYQSEQEKQKIQNRQAQFLPFFEKQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TGW7</accession>
    <name>A0A8S1TGW7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0240166</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TGW7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8153361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="66" mass="7919" checksum="E74431BA209CBC99" modified="2022-10-12" version="1">MSYLSNDNNKITIKIIDRVQVICFFLRNLFGRNQINDKGIMYQNQQKFEIQQKKFIAQKLILVLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TJB5</accession>
    <name>A0A8S1TJB5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CHCH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0250252</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8154201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TJB5"/>
    <dbReference type="OrthoDB" id="310526at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055304">
        <property type="entry name" value="CHCHD2/10-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13523:SF2">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13523">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 2/NUR77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8154201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="132" mass="14106" checksum="08043FEDC8EAF54F" modified="2022-10-12" version="1">MPRRSGSASRSRPSPSRSTATAARPAPQVQAPPQTPAKSGGMFSGLGSTLMTGMAFGAGSEVAHQAIRSVMGGSSHSQPAEQQTQEQAPQQQQQACQSESQMISNCLQTNSDITRCQPYMDIFKECQKKYNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TL75</accession>
    <name>A0A8S1TL75_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cilia- and flagella-associated protein 157</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0230076</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TL75"/>
    <dbReference type="OrthoDB" id="441129at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14845">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING 166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14845:SF0">
        <property type="entry name" value="DUF4515 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="416"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="4"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="190"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="301"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="418"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8152487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="440" mass="52641" checksum="55742E43ACF9F797" modified="2022-10-12" version="1">MAKKKQQIEVVEFTEAEKKALEKLDHSHLLIEIQVLQDRIVELQKKIYELNENSRKTNDQNLTLTLKMESDIIKKESEYNMLLQQYDDLRKQTTEEIESVIQRKDDKIKYLEKEKTEQEIKYKDELQKINEELKKWSHFKDEKENLIKEIHDLKEEMTKKDYAHLDEQSKANKNFLKQTQNLTNVNNAKIEDVQRNANKLAEEKVAAKYDGILKENEKIKNELLEYDRTLQIYQKDKETFQQENINYKLNLSIKEEQAQEYQLMNHQQIIKIKQLKDRIEYLKSYIANEVTKQTKEIEIMKHQSNTKVQELEIQLRNLRNLLDQRTKELKTVKALAQMILDQRSDIEQFFLQAIDQVKNEMKIKNKNQKNSRLPDISQKSQSLEQNKVDINDLDLEEKEKLLRILFSKMNQGIPPINWKSQLGHSKSQVDASSFRDNQYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TNB9</accession>
    <name>A0A8S1TNB9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="18">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">PPENT_87.1.T0230233</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 18">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Membrane</location>
            <topology evidence="18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="18"/>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17484">
        <property type="entry name" value="MFS_FBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000180">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000009">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039309">
        <property type="entry name" value="BT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004324">
        <property type="entry name" value="FBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00788">
        <property type="entry name" value="fbt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF5">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03092">
        <property type="entry name" value="BT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9 17 18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 16 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 18" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="9"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="102"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="318"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="348"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="384"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="532"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="555"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="747"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="779"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1278"/>
            <end position="1300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1306"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1356"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1382"/>
            <end position="1403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1410"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1443"/>
            <end position="1461"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="20">
        <location>
            <begin position="508"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="19">
        <location>
            <begin position="597"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="21">
        <location>
            <begin position="1243"/>
            <end position="1439"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="15">
        <location>
            <position position="838"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="838"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="838"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="839"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="840"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="840"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="934"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="975"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="999"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="1030"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="1110"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="1111"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="1112"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="1191"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="1197"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="1217"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="1220"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="1221"/>
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        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="1221"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="CAD8152772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1482" mass="168706" checksum="A8A1F01BA9F8D0F2" modified="2022-10-12" version="1">MIKQSHSTYAVICLVAFSQGVINLSELAISYLLKEDYGMNPTEMTYIQGIISIPWVIKPIWGLCTDLLPIYNYRRKSYLFIFGLMGFVLFYALSIYGTKDAVAGVSILLSIQICIAFCNVVAEALLVEISTGNDSSTNNVSLFFGFKAFGTLMTSYLSGYSLKYLQKQQIFRITSIFPLIIAGASLFLNEGQHKDTDIKKQLQELHQFLNIKAIYKPIVFIFCFMLQPSTSTAMFYFQTVYLHYTAEFLGKIKFLFALANITAVSIFNRYLKNFSFKSVFFVTTLTYSVVNALQIIQVTRKNVELGIKDEVFSLCDTLLMQLIAELNMLPILVLACKICPSKIEGTMYALLMSTINLGQLVARQIGGILMYFLNINEQSFENLWIMITMTSVYILILLPFLSTIKEKEMIESKDLYNKQDIDEFDIVGKGYQRLSLSFAHSTNLLIEMVERVDDDKFLDRKLEQECDSFNDKIDTRSLILKAIDIFGKQKVSDDARIIEITGEKQPQSIPNIVKNQKYSIITFIPIVLFDQFRYFFNLFFLLITLSQFIEPLKVGFLFSYVAPLVFVLTLTLIKEAYDDFKRFLRDREANSQLYSKVLQNDVISIKSSQLRVGDIIEVHANQRVPADCVLLRTSEKSGTVFIRTDQLDGETDWKLRSALKLTQIGQFFGSTLIVEAPKLDIYDFKGRIKIDEDKESVSIDNTLWANTYVAAGKVTCVVIYTGRECRSQMNQRKSRLKMGRLDQELNFLSKVLFGFMCILAGGILILTPDNDVLINLLQFFRYVLLLSSIIPISLRVNLDFSKLVFSMKINSDKDIAGAMARNSQIPEELGRVHYILSDKTGTLTQNCMTFKKLALESNTYTTKDLNVVNKILKRQFSANPVPLHDIITGKTKRNSRRNKDIVLRDLIQCLSLCHNVTPIIEDGIRSFQASSPDEIALVQISEQMGIQLISRNSEQMVIQTPNKIETFKIIYEFPFSSERKRMGIIVQSDSIDGYFFYLKGADQIIKNKVPEVQRGFLMDEAEMLSREGLRTLVMTQKYITSEEFKKWQIDYEEAKSSMEDREIKVNHVLEQLEQGMEFLGISGVEDLLQEDINHCIDQLRNAGIKIWMLTGDKVETAQCIGISAGLKTPQQEMFTIKEADSLNLQNLLNQFSSKHNSILVIDGVSLTLAFNDHFDQFMYVTSRAPCVICCRCSPTQKAQVVEAIKKNTNQVTLAIGDGGNDVSMIQAADVGIGIVGKEGKQAALASDFSIMKFKDLSTLLLWHGRLAYKRSSVMAQFVMHRGLVISIIQAIFSFLFYGIAIPIYNGYLMLGYATVFTVLPVFSLIFDEDITKNKALEFAELYKSLQKGREVTTKTFLIWLWKSIYQGTVIMALSFLCFQNTFLQLVTVTFTALIVIELLNVITELKTFKFITLISQFISISLYLCCIYFMRDIINLSEITVNFVINVGLLVLASWLPLHLVEKAIHKYYPSEADKIMEKIKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TQA2</accession>
    <name>A0A8S1TQA2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Splicing factor 3B subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0250266</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SF3B4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8154228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TQA2"/>
    <dbReference type="OrthoDB" id="10259687at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048026">
        <property type="term" value="P:positive regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12334">
        <property type="entry name" value="RRM1_SF3B4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12335">
        <property type="entry name" value="RRM2_SF3B4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000059">
        <property type="entry name" value="splicing factor 3B subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000121">
        <property type="entry name" value="Splicing factor 3b subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034158">
        <property type="entry name" value="SF3B4_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034159">
        <property type="entry name" value="SF3B4_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052084">
        <property type="entry name" value="SF3B4_spliceosome_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48030">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48030:SF3">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="19"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="106"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="237"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="246"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8154228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="261" mass="28959" checksum="AD2AC37EE362DBE7" modified="2022-10-12" version="1">MSLALAGVVPPLYERNQEATIYIGNLDQKVTDDIVWELFIQCGPVVNVHIPKDKISGEHQGYGFVEFKSEEDADYAIKIMHMIKLYGKPIKVNKASQDKRTQEVGANLFIGNLDTEIDEKTLYETFSAFGHILSTKIMRNPETGVSKGYGFVSYDNFESSDGALTAMNGQFLGTKIIRVEYAFKKDAKGERHGSQAERLLAANRPLAQKALLGFVGYMPTELRIPLPPPPSIQIQPLQEGYKPLPQQLPVPLPPPPSGMPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TXD7</accession>
    <name>A0A8S1TXD7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0290195</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TXD7"/>
    <dbReference type="OrthoDB" id="289272at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF110">
        <property type="entry name" value="DEVELOPMENTALLY-REGULATED PROTEIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="394"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="9">
        <location>
            <begin position="653"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="140"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="231"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="34"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="140"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="152"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="423"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8156840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="902" mass="105023" checksum="DAD18242AF8CE6AA" modified="2022-10-12" version="1">MKKISQQMLTLMNKNSLQDKPFNNDLKKNKSKPKTVNTERPQSKTQKNDLLEYFKKERNTLTQVANSSKGILQGSKSIDNLQQLSQKSERLKTGQFNVDVRIQTDDGNSAKRQILKQKNLSLATISLDAQNIDKMLKNQLSKQPQTTKQSYNQSPQNQIKQPIQKQKDQLLFDMHLLKNTVCSNECQNYLNLKQNKQSIQSNTSSNHRQSIEDMFSSQKLLKGSIQGSIQYQSQKPKKNESESKSNNQSHIKTEYSSGSNQQFIKQKCKEDTVQYNINKQLQIILYYKQQKYNYLYEYQNQTTDDMYNYLIQQIACIERSFTKLGSGTGSTEVDQIQQELNKIYQFQTVSKNIPYDYYLTIPDLTLDVFKGITLQLQPLYTQPITTKRVGLKDFTLVKCIGVGGFSRVYMVRKRDNGKFYALKLIDKKFILDNQKEIIVQNERDIMVQMENQFITPLHYAFETKYYIAFVLDYCAGGELFYHLRKLKRLNEQDAKFYFVEICIGMAYLHSQNIVYRDIKPENILLDLHGHLLLSDFGLSKPDMSPDDFAYSFCGSPEYMAPEMLMKTGHNYLVDCYCLGALLYELVTGLPPFYSHNTQEIYNSILTETVEFPQYVQISPVLKELILRLLQKDPTERLGYNGGIVEILTHEWFQDVDFEAIANKKLKTPYKPEPLKYNFDEEEFNKGDAEFRKQYQTNMQKEYHNIDTANYVLKNFYYSRESQVGLEKTKRTNEVNLNQLKNQAIPIESQSPDKTLKVLQQQKGEYYSQQSVLASPQDGKRINRQTGKSEVYDYFKKHDLLTKSSQLLHQSTKLGHSYSKTMQQAQNSLQDLKQFKLFVDQSKALICSDRTTTMPDQNHKNITERIKTEQFGNLPSPKTTTNSSLNKLNNFSKLFVSEKQKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U0R3</accession>
    <name>A0A8S1U0R3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0300081</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000030">
        <property type="protein sequence ID" value="CAD8157297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U0R3"/>
    <dbReference type="OrthoDB" id="433501at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044640">
        <property type="entry name" value="RU2A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552:SF6">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8157297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="218" mass="25427" checksum="0FE964A5621F0B4B" modified="2022-10-12" version="1">MKINSELIQGSHHFINPLNERQLDLRAKKITAIENLGATLDFFDHIDLGDNDIKKLGNLTLLKRLKTLNLSNNRIIKLTDVSDSLPNIENLILMNNRLTDINELYQLKHCKKLKRLILHGNLITQQPDYRYKVIAILPNLKILDFNKVTQSERQKAEESFKPDELTDYMNLINLKDATLDKEHIKKLLENAKTFDQINQLEMLLKQQQVKHLSILNEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U1Q1</accession>
    <name>A0A8S1U1Q1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0310344</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U1Q1"/>
    <dbReference type="OrthoDB" id="270720at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="20"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="50"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="86"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8158448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="354" mass="40056" checksum="183DF50CAF25DAAE" modified="2022-10-12" version="1">MGNGCSCQCQAKGMDEVELNTNQKQQSQSKPNAKMDSCHNQEADVQEGNNKQKKEKIGAHLKDEDVAQMAGARNNSIEDSSSPKLMKRENDDNKPEDQYISLVQSTKGKLTRTKLSAIEMKNGGVYEGEWKNGLRDGQGKYVWPDRSFYEGEWVEDKANGFGKLVHVDGDIYEGQWLDDMANGHGVYIHSGGARYEGDWKNDLQHGQGVEAWPDGAKYEGKYENGKKHGQGTLTFADGSYYKGDFVENDITGYGEYYWKDGKSYKGQWNNSKMNGKGITQWADGKRYDGDYKDDKKHGFGIFQWENGRKYEGYWINGKQHGKGMITLPNGEKKEGLWENGKRVKWLNVDESPNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U532</accession>
    <name>A0A8S1U532_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0350058</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U532"/>
    <dbReference type="OrthoDB" id="302751at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00060">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46210">
        <property type="entry name" value="FHA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46210:SF1">
        <property type="entry name" value="FHA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="1">
        <location>
            <begin position="343"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8160581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="455" mass="53303" checksum="89962A825DA38620" modified="2022-10-12" version="1">MSSTLNFSQILNNHLNNIMNPQSFIQPFNLQQLQRNGTATNGNENEMNIENFIPNNISSQNGNDQNIVALQTIKSEYNRDSPHLKINVYKQHQQTNQKTYILQDDKIIGGNQIHKNEIQIIRRRKTSRSSENNLDIGLTPIESNIDQVPCKISTEFGFKYSSRITPQILVFLSLKQFNSNFAALPSNVFKLIHQFIKEQPKFYIQDCGTSLKTLVRIQKDNPRIMNENNKYLIGADFYFHVVQLSTYNLKFEDKRQQETNTDYFYQTLVREHTRDRARIHGLSKEEQDIFQKYLQNYIQMKKKGRKQYSLENCNRPFLKIQFDTPIVKQMAIFIARPGGSKIFKIGRSQDCDIIVNMNTVSRKQTQIKFNVNQWEICDGEGIKQSANGTWQSLQQYENPLNPTQQKISQPLRIEDKMEIKISENIFRFDMVGFGIQKRIKLNNQLYKDLTNIDLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U5C8</accession>
    <name>A0A8S1U5C8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0340042</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8159884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U5C8"/>
    <dbReference type="OrthoDB" id="341421at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10527">
        <property type="entry name" value="SET_LSMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1410.10:FF:000038">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015353">
        <property type="entry name" value="Rubisco_LSMT_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF153">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09273">
        <property type="entry name" value="Rubis-subs-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="1">
        <location>
            <begin position="45"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Rubisco LSMT substrate-binding" evidence="2">
        <location>
            <begin position="550"/>
            <end position="644"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8159884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="697" mass="82275" checksum="B2F24046A7B1E131" modified="2022-10-12" version="1">MQFDQSKTNEIQQNPKYKMLLQWGIENGVLMKGVHFPASFGNVTGVVASEDLPSDTAIICIPQSLIISPQKCKQSILNTVYNNHPEMFDEEENNEAEFNILTFYMFNEKKKGELSFYYPYIQAIQTNNTLLAWSNDDLKNIEDPIILEEFQLIKQDFLGLWSKAKLIFDNAQDVFGAPRITDKSDFYWAIECVTSRCFGWALKSTCLIPIADFLNHSNQACTHYMVHQGLEKGNDLKSVVFQQQYIIKRNQINLSILGIEDSREQQKWEDQKNKFVIQNKQYIKDLSIIQDLDRVSIQEKRNIINMIHQEQILQDSQMNIWDLISINSSDSEDNDSDEEVKMAKQQQFEILKIKELAEWKIREEQKRIEKQFQQINLKNQPIITVTLNPEKKNKVIIRGLPQYQIEAIKVKQQMINGRLRKQQDESTQSKDNEKEIREEQQWDWLDEYDKEAYFCITTTVPIKKFEQVTVSYGNRSNRFLLCWYGFALPDNVYSSYNFRLWLNTEILEDQEKSIDQILNTIIIKKLIHEEESIIDKIQYNGYEIPISSLSKEFRIKKDKLNMDIISFLRLLLQLKYGNEKDLLSTIPVSIDYEILVMQFYNSLLQYLMNSYSQDLTQDLKELEQTFEYPKRFAIYINKERKEILIKQSIIVTEAITILKKFKDTQNLRDSYISNLTSQRISIIKGLKEYLKLLNEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U6G2</accession>
    <name>A0A8S1U6G2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0330271</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U6G2"/>
    <dbReference type="OrthoDB" id="300840at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8159683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="241" mass="27992" checksum="ACA9AE11A99F32C9" modified="2022-10-12" version="1">MSILVRVEGASKDLRIMYPEPEEIQLNFCTLIDLEFTSFSNKEEVIVQFKLKEVVALKIQTQEVLLQLDSIRKRFMLQCLVYPGEHMVWVSSMNNYVVTEKSLDHVLMGKKPQLETITEEDDAVSPIKIHGKENKPIGQKQCHSKKISPVKTKSPKKKDQSTKRTKKSTLRWSQYMVQTPKSPLKGDYVDALLAQLIQQNNRKSKSRKRRPSSSYPLRPKIDSEGMIDRQWLEEEMINEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U8G7</accession>
    <name>A0A8S1U8G7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0340238</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8160242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U8G7"/>
    <dbReference type="OrthoDB" id="305764at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="212"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="255"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8160242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="323" mass="38212" checksum="BE54120157BE0245" modified="2022-10-12" version="1">MATNINGILSCCQDFGIYCITLCISEVTALAVLAVGICQLIRRKKIYRQAKKRKNIIYILIILCLTCNILFNLAQKTLLVFYFFQLYLYYAFLIYIVFYFARKTLSIRTFKPHQILQLKIYCICLILLFTIFLIWNLYDYITSLSQSYSLCQKQIFHIIRMIGFIVNLVLIAVAHSLNLSIKKNLELKSTILKNFVNFEENCKKINRQRINFWVLVYVTFFGQLLYFIEDIYFLVQGPTETCILYLDIIQSEARIFQYCLLLLVVLIQVQLPFSVTIYAFWFQKPIIKQESDSIEFEEDYFRTIKLQQKNDSNSSEQITTLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U9K1</accession>
    <name>A0A8S1U9K1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0350039</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U9K1"/>
    <dbReference type="OrthoDB" id="2127950at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00652">
        <property type="entry name" value="TBP_TLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000814">
        <property type="entry name" value="TBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10126">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00352">
        <property type="entry name" value="TBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8160543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="312" mass="36222" checksum="2B2F338FE41CC8D5" modified="2022-10-12" version="1">MNLYYYFDDGSTEFIRILGTLKPEGVFKSFSYIQINFFNFYFLNASREHLGNLTLFKYQNYDYIISISLDFIIFLQISKKSKHIKKNKTDVHEYTEEKKKEIEKEYLQIKKNNQLQESYEPKIQNVVSTVILKMKTKAGEEIDKLDLDNLAHTSQNCEYKKSRFPALIQRIKEPKSTALIFEAGKMVITGTKGQNEAEEAAKKFRKQIEKNNRITIEIGDIQTSNIVANSQLPYEVNLMKINDDRSLSGSISYDRSAFPGLIFKMQNPKLAALIFYSGKIVFTGAKNETQIRDAYCQLFNILKKHSQEKANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UB20</accession>
    <name>A0A8S1UB20_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0350224</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UB20"/>
    <dbReference type="OrthoDB" id="300402at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8160892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="155" mass="18279" checksum="D01F206BAB97B404" modified="2022-10-12" version="1">MKLAILPDPIHRQYFANHVNSLKQIKSRKNKIEEDSLGVIRQLSTIKKIKDQVSAFEAIEENKSIAYNNQKIQQAIMKISSAKHNLPKLHIKRKEKTEISDEIAKENKRLKKSIENVQNSLTYRTKHQNIRKSYQQVLNEYCQSRLQQQNEQLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UD06</accession>
    <name>A0A8S1UD06_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0360282</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UD06"/>
    <dbReference type="OrthoDB" id="287831at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8161629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="295" mass="35437" checksum="E1CAEA4F3A2D8A7B" modified="2022-10-12" version="1">MKPQKSIIKELKTYQQDLYKSGREGKQFSINDFPNLSRKPIEWTDCDQFIKLRQMKCDKKLAIRLCQQYKIHRTDQLFFDTLHNVNMENIGYKKIQSSTSFLLRQIRQNQFIKQKYRMEFQDTQIDNKMSSILDDSFQKFQKSLDKHLEIDKDQSHKFNLKQMTKEELYSKLQQLQFISKFKKYYSKIKSIHGSFTFQQFHSSFQFSHTFRKLSSNVSIKQQFTKLQNNQLNIQSFKLIKQNKLGRASFNPQVNVAKEIKSMHHIGHLNDMPELQKQHKRHFTNYDITGNIVNLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UDS4</accession>
    <name>A0A8S1UDS4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0350161</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="296539at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058584">
        <property type="entry name" value="IMB1_TNPO1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041653">
        <property type="entry name" value="Importin_rep_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057672">
        <property type="entry name" value="TPR_IPO4/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18808">
        <property type="entry name" value="Importin_rep_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25574">
        <property type="entry name" value="TPR_IMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25780">
        <property type="entry name" value="TPR_IPO5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="IPO4/5-like TPR repeats" evidence="9">
        <location>
            <begin position="109"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Importin subunit beta-1/Transportin-1-like TPR repeats" evidence="8">
        <location>
            <begin position="489"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="695"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8160766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="695" mass="79060" checksum="989198BD9A4C6D85" modified="2022-10-12" version="1" fragment="single">MFTNLRSTDNTIRQQAEQELYTQVAANPIAILEQFLIAMQNKDDTEFVSILLSKTIFENEENIGKLEWSHVEAVLKFCVSELKQENKLSHLKRMCDLAVKGLLKFTKYDEMIMLLFEIGRSAQTLNQKLATMYYIEIMCEFALCDEQLIKHSQQLNEIFSAFLNDQSPQVRAATCQGISSFLVSIEEESLLNRFSNNAVVLLQQFAQVMQVDQEASVQALQSINELLENHPKFMKNLYGELLNIYTQLMGSQTTISLKKSALHGLQTLCQIAPAFIRKSDQFKTSSILMIMKMLTEVDRKDWENTFDDNCLQLNDLSSVAEDCLGKMVRDLGVKYLLPIFVPLIMQALRSPVINEQHAGLIAMATLSDKAAEHFQNELPSIMELILPLSQSQNKLIIYDLLTCLASLCQEFTPKIQINYGSQILQLIVTCMQQKISQKIQYISIACLVDFTRELVEDKEAAKNVLTPVSTFLIEQLYSVIQTNLTGSIDQQQQQILEQALSAFSALATSLQEHFTQYYDQMMPYMMQMMQTVTVNEVKSLLLECIGCFLVSISTTRKEQCKTDSNQLVTHFIQQQNKMESDDPAHTSIFFFYTQVATALRCEFGQYLDAIFPLVERAMRLDVGFSINSQAEGKNITKVKLDLKFLGMKSLSLNTSALEQKVEGAHTLVNLAEQCGKSFYPYITKTIVLMKEFINX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UDZ1</accession>
    <name>A0A8S1UDZ1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0380115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UDZ1"/>
    <dbReference type="OrthoDB" id="306646at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8162554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="262" mass="30991" checksum="CF03D0D9A2302CBD" modified="2022-10-12" version="1">MKRSSNKLQKKNASQPQTENRGKPSQLKLLQLNAPEIWTNQHKCLQIIKYLTDTGRHNFLHQAGKHSNKDVDHENQQYNQDTKIYFEQAQEIAEKLFNIQCSSKRCIVFYFRYINEEKITYDFSEYIQNKDFVEILSSIQLLLTKSFIQDLIIILSSHNSINSITDMLLENKGAPSNYSHIIKNFFIQKKIDTMIEQKTITPDIETIRNEFEDFLKDPQTKYLVRVFICDILSIFYTQTTDQSNLDEQFEMAEVNDFDNIYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UL50</accession>
    <name>A0A8S1UL50_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cysteine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cysteinyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0420059</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.16" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UL50"/>
    <dbReference type="OrthoDB" id="438179at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004817">
        <property type="term" value="F:cysteine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006423">
        <property type="term" value="P:cysteinyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00672">
        <property type="entry name" value="CysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000027">
        <property type="entry name" value="Cysteine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00041">
        <property type="entry name" value="Cys_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015803">
        <property type="entry name" value="Cys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024909">
        <property type="entry name" value="Cys-tRNA/MSH_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032678">
        <property type="entry name" value="tRNA-synt_1_cat_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00435">
        <property type="entry name" value="cysS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890:SF3">
        <property type="entry name" value="CYSTEINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890">
        <property type="entry name" value="CYSTEINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01406">
        <property type="entry name" value="tRNA-synt_1e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNA synthetases class I catalytic" evidence="12">
        <location>
            <begin position="53"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="576"/>
            <end position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8165134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="682" mass="79275" checksum="B5390929005BCCF5" modified="2022-10-12" version="1">MIKFATRTINQFMQQQKDLFAHYKQPSIKEDSYVTGLKLANSLKGSELVPFIPINGRQVNFYYCGPTVYSNSHLGHARTYLGIDVIRRTLRDYFNYDLLSVMNITDIDDKIINGAKQAKQEFLEFTKVWEKDFFSAMEALNIELPDVITRVSDYVPEIVTFIEKIIANGYAYDSNGSVYFDVQKYLADGHTYPKMRPEMNADLLQEGEGESQANKQSEKRSPNDFALWKASKPEEPKWPSPWGEGRPGWHIECSVMASAILGNPIDLHAGGIDLIFPHHDNSLAQAEACFNCDQWINYFIHLGHLNIADRKMSKSLKNFLTIDEVLKRYTARQIRLNFAIHQYDAVMNYSEEQVEQAISKDKAYQEFFQNTKIYLRESQVTGPQKWTQNDFLISNSLRSTKQVIHAALLKNFDFPTVIDAIDKLINQVNVQIANKTVRAPLIQSVVQYVHFILGLIGLNYQTQNNSSSDIKFIMAEVCSIRDQVKLAARKGEFEAISNIVTQKQFQYEKNLSNGIVDCINQFQQQVLAASQEKNKQQLFQLCDKLRDEQLFELGIKIEDKDKEQASIWKQYDPKELKLEREQQQKLIQQKEQIKKKEEEKKKQEELEKLEKAKIHPKDMFLSQLDKYSEFDEKGIPVKDKEGNELSKKQKKVVQELWEKQNKIHNQYLESLKKQQQQQQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UPB6</accession>
    <name>A0A8S1UPB6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0460039</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8166731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UPB6"/>
    <dbReference type="OrthoDB" id="299284at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8166731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="123" mass="14021" checksum="6087F87F61908FA0" modified="2022-10-12" version="1">MFCCSNAKKKLAPPTQNRMNNHQNSSEQTLNINDEAFTFHTTIQIQQINVTHNYKITQDLQTIRSSRSNSRGTKNNDHVAQSVLVKPSRKSPFLTFNKKGTDSTTKYLSTKQGYTLLVTRNTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1URL4</accession>
    <name>A0A8S1URL4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0440135</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1URL4"/>
    <dbReference type="OrthoDB" id="20534at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045234">
        <property type="entry name" value="Unkempt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057444">
        <property type="entry name" value="Znf-CCCH_AtC3H23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14493:SF50">
        <property type="entry name" value="RING FINGER PROTEIN UNKEMPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14493">
        <property type="entry name" value="UNKEMPT FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25512">
        <property type="entry name" value="zf-CCCH_AtC3H23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="71"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="71"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="316"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8166412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="473" mass="55880" checksum="2FEB7FB59BBEC9C5" modified="2022-10-12" version="1">MLGSRRVRSHESSLTNLDAYNPNSPVRIQQHINHHQFKTQRCQIQHQINQKKFCPFFHDETDRRRDLKYYSYKCQLCPQADNCPQGDECQFAHNKVEQVYHPNRYKTKYCTHIKDCDYGFYCSFAHNDQELIIPVKLDGMVQDKNFWMYQYKTVWCPLTINHDRASCVYAHNAQDFRRDPKKLQPKECPHWNKTNQILNYDKGGCPDQEECQYCHGWKEFEYHPLIYKTKPCTQTNCNKKLAECAFYHSDQEKRIRKQLADNQWVMEEPNIHVEAKRQPYKPTSNYLGPIIPNYIPQDYMSKEKMEIGQPFCQQSITNTKTSDSNSRRGSDFSDGSKMNKKKHNIQQQFQPQKKHRTAPTTPDQKQLHIMGNNYTIKAQQNVQSNSHLSYSKKLYEEILKLNEGECIYKILQGFKISEQMLMQMGDQQIKQLNLSEQQIIQLTSALAAIKIEKKYDEHCGDELLSLISNQGKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UV31</accession>
    <name>A0A8S1UV31_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="5">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0480191</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="5">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDO010000048">
        <property type="protein sequence ID" value="CAD8168107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UV31"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07834">
        <property type="entry name" value="STKc_MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000815">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000098">
        <property type="entry name" value="Mitogen-activated protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="41"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8168107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="427" mass="50101" checksum="D8BB0BB3509594CD" modified="2022-10-12" version="1">MQQEFKLPQKIIQLYAKYQNKSQKVKKFQLQEHTFMLEDNYSPVEIIGSGAYGCVIQVDDKNAKVEKDRQVAIKKIERAFEHRLYAKRTLRELKILRLMKHENIVELKTLLLPKSREEFEDVYMVTELLETDLAQVIKSDQVLTDEHIQLFLYQILRGLKYLHTAGILHRDLKPRNLLLNRNCDLKICDFGLGRAMADPSSSNNANIMTYYVETRWYRAPELLVSFKNYTPAVDMWSVGCILAELLLRKPFLRGDSTKRQVKLIFELLGTPNEAYIQSFQDEKVQNNLRKVIKETGPKQGIPLEQLFKNASKNALDLLRKLLTFDYRQRITVQQALEHPYLAQLHFEADEPSAQLVNQLEFEFEKYEMTREQIKDLLYEEILLYHFPEFQTSYEAKKKSGQSLISHVVNNENAKIFDPTADDDLDRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UVJ5</accession>
    <name>A0A8S1UVJ5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0490008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UVJ5"/>
    <dbReference type="OrthoDB" id="305569at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="25"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="135"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8168267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="243" mass="29599" checksum="8CD73E4E80E50324" modified="2022-10-12" version="1">MRTSQSENITCTALKHLKNQKLYEIILLKERVALLQKESQNIEQRANPLKSKLLKNVEESKRLYEDIKQKEQEYKLFIQSEKEKEYNAKRQKKNFIQFSSNEALKFARQHVQQEKKEQAYQIKQLHQQDKLMIKKNKDQQMNEKIKKKCQVLNEEKQAQQKIKEFQQIKQENFQKITQFQKLLIQQDLYSQEQEIKALEEQEMRFLQKLQQSKSQQLQFKNQVKDAMELNVEDYKKKYPKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UY79</accession>
    <name>A0A8S1UY79_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0470088</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000047">
        <property type="protein sequence ID" value="CAD8167376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UY79"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8167376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="90" mass="10974" checksum="50EEE38F84D2742B" modified="2022-10-12" version="1">MKQFQQQLKNLDEIIQNNKNSINFMRKIAQEYQIMLKRMIQEFISDFLILQIKKYILKSSLNYEKLLRFKSKIKTSSINQKVEQIRSFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UZ82</accession>
    <name>A0A8S1UZ82_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">U3 small nucleolar RNA-associated protein 25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0530020</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UTP25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UZ82"/>
    <dbReference type="OrthoDB" id="10264378at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010678">
        <property type="entry name" value="UTP25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053939">
        <property type="entry name" value="UTP25_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053940">
        <property type="entry name" value="UTP25_NTPase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12933">
        <property type="entry name" value="ORF PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12933:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 25 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06862">
        <property type="entry name" value="Utp25_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22916">
        <property type="entry name" value="UTP25_NTPase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UTP25 NTP hydrolase-like" evidence="6">
        <location>
            <begin position="266"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="UTP25 NTP hydrolase-like" evidence="6">
        <location>
            <begin position="318"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="UTP25 C-terminal" evidence="5">
        <location>
            <begin position="468"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="33"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF22916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8170360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="647" mass="77226" checksum="C7ADCB0699896D3F" modified="2022-10-12" version="1">MGKKNKKLRNQESDPIVKQIRKKKPQSIKNQEQVEEITDDEEISEQQNEDDQIFEHVVDFKNTNEKPSHYQNLLKKLNIKQQQADDNYGCSESARLNIKQENKSVYAKMKKYELVKVPNLNPFSHFEYDYQELRKTICIGDIEIEQPEQLNAFFKEKTTKLINTLNNGIEFKKYELNKQLKNKTLIDLNCKNEDYNIETLSQRQLIKDDKFLLPVVKDSEEEKELLQSNLIYSYFDYIETDPDEQNAEKFRKTYITHISNHLDKEKESYGFTRCKVLILTAFKGDAISIINQLIQTDKEVVNKERYENEFCDEERLEEDDFRIGIQFSRQGYIKLHANFDKSDIIVTSTLGMRLIIGTEDQKHRETHFLSSIEILVIDRASSMYMQNWSYLEDILNATNLLPNHKNITSDLSTIRPYCFENLTKFYRQNIVYTDHYFQELNFLRKQYFSNYKGCISNKIYYNQLFQKSVNFQQEFRQIDCSNTEQQIEQRFNQFKKLWNDIENNKSSQFKKTLIFVQSYFDFVAIKGHFKRINSNCECVSEYTKKNKVQSIISKFKDGRIQYIMITERAYFFEIIQLRSMKNIIFYQLPQHSIIYKQLIQGIENLGNKTQIITYYSKFDAYQLETIVGTLRAEEMISEQNKRTTFIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V3T5</accession>
    <name>A0A8S1V3T5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trichocyst matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0550089</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V3T5"/>
    <dbReference type="OrthoDB" id="299305at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035913684" description="Trichocyst matrix protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="231"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8171565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="359" mass="40360" checksum="67FBFAC9FBB571AC" modified="2022-10-12" version="1">MQKLVILTLVVALAASKFIDTHTTLAQIDANPFGNVVLSAIKAHIQAQTPANEVNMLLNGVAAGLQQDQNDHDHAFELDTTTNNRIVEDLEKEILYHQNQISSNTQLRDDTIEALAVSEEDIRVTIQDIATNEQTYAREEATRNQQHETFVAKVAAIDDVIDAIDEAAKLIQHLSLGASFVQVKSKYENIHKRLTDNTSHSQLLQPVVAALTELATHGVNQKALTKIAQLLSEIRQQLVSEKAAKTDVEERQAAHWAEFSAHLSNEHTRLVERKAQLEVQIQEQKDTIEDAQSWIEFHTLELENSEERLAGQQAWYAVQSEIYETQTAERAAQNEIVDRLQEHISEKLSTTAQFISKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V3Y0</accession>
    <name>A0A8S1V3Y0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">STAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0540144</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8171163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V3Y0"/>
    <dbReference type="OrthoDB" id="288203at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="64"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="204"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="227"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="355"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="378"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="419"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="6">
        <location>
            <begin position="483"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8171163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="578" mass="66695" checksum="381EE814A7741DBC" modified="2022-10-12" version="1">MNKTPLLIEMDQIKEEESHKVSGVRQSKFSIVSRLSVQDLQSAWYYIQETWKSGMSQAALNMPFVLALGANSGVSAAMGLTTAFIAAFVNGFFSGSNHSIYMPSWTITGWNYVLTQQYGIQILPLATLVSGLMIYLISLFKWHQLSDYIPQYVIEGFYLGSGFLFLILYSDYIFGITDNHTNVGQKLYSNLIEMYISYYERGDLYNVIYTFLIFMFLQFGYKFHRPFPWVLLTTSFGLLIGNLYPTEQCLRYVYGNTRLQFNFIQINELNLGFESILEIIQLCIPIAICVTIQNLLCARGAQSLTEVKCDYDQETFCVSFANVISGLVGGIPCCASSRMYTLNIRLRDINKWSSIINACSIFIFYGIASQFFMNIPLYVIAGQILLVVYQFPPWNYLIKLYQTKRRLTLFKCILISWICYNYGVMYSTIIGCLHSLFLFAQKMSGAASEIIVNTDHDPRRSSYIEKEQHFEDDLPKVDGSYTLYRFSGSINYININNHINQIKQLAFTDYIIFSFRYVSVFDEQAIDKLAIMIENLMKSQHEIYLTGLHKTMIEEMEYNSFFKEFFILCHDKVKTLGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V4A7</accession>
    <name>A0A8S1V4A7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0560148</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000056">
        <property type="protein sequence ID" value="CAD8172200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V4A7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8172200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="229" mass="27336" checksum="2D427C443200FDF2" modified="2022-10-12" version="1">MINGSYFQVGIGYRDIMQKNNYHQCYYTGTYLIQNNGHIFSHHSKDLLDKNLSFRFTNNDIIIIAVCIEHKYIKCSRQYNPQLTFVQLDIDTSQELNPCVGVSQQSKIQLLDNMAILRQIRFKYIFKYLFIIQNNQIQEVKQLYFQQKQIAIIQEHKLYYNTAQNREVTRAIQLYCRYSPYKLIFLSIQIIKCKANQNLWEISEQINFGESIEKLVEEKKIKLVKEVFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VB69</accession>
    <name>A0A8S1VB69_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0600049</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000060">
        <property type="protein sequence ID" value="CAD8173773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VB69"/>
    <dbReference type="OrthoDB" id="304909at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="841"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="42"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="167"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="387"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="490"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="845"/>
            <end position="879"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8173773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="879" mass="105437" checksum="A39B850648EFE82F" modified="2022-10-12" version="1">MDHSPNRPSVSPLSKTRMQSEQTLKLQDLKYKEKLLQMDKINAKLQSQLDETLLELEKANKNLHLQNQMKETQDNEYLRLLKENELLKGDLQFKLQENELNKQKLAQQNKQYQSSLENLKEQYEQLQQSLMNKYIIEMNTKLQDQKQQYDTFSKLQTERMKENEHMEKQYQEQILDYKQSIQEFQIKVNEQKQQIETFQKQSYIDKQMIADKELQEQLLLKKVEQLQIELSRLTTDIDISKEKYLRLEKQYEQNITNIVQSKDQQAEEQTSKLYQQHEQSIQNLTLQNQDIWKQLQEQKSINQVQQKEITRLLHTNQQQGQDLIEKDKKLKDLSLNTNQSSEQCIILQQRLLQNEQALQLYKSQLDDFKQTRGDFEQRMMERSKGILRQKEQEFQQALQMNEQKIDDLTQEIERMENEYQNQSFKQLQEKLMSESHVKSLLVKIAGIEEQNSTLLSQLEETRQELLQRSTLSENLSVQLNKEKTYNQTTLRIQKNQMSDLQQKLKEYQVEVDRLSCDVRERDDILENQRMQFINEKNELEQQIKIIFEKMSQQRQRIQELDDQNYQQQCQIQKLTIERDKLDKDLKSLTSTTNEQIRKQKEQIDNCNKLIEENQLTILQNEEQIQNNETQIAELQNQLEEQYAQNQQLTKDVNELNDELEETRQDREAKIQEMDQWKRQFKQNNVPLSDYDQVKKEAELYKNKSMDQEQLINKLETQQQANNKEIYHLQGELSQYKSNLQETQSQLVNKKKENDNMSTELENLKREQQRTLNQLKEIEQRDQQNSELVIKQKKEIQRLNDDLETKNRQLGNMEQQLNKKFDEVATKQRELEELKRKFQQDAFGKMTTSPSQKSVMMASQNMAARSTAKGLTTKISEQQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VDF0</accession>
    <name>A0A8S1VDF0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cation-transporting ATPase</fullName>
            <ecNumber evidence="11">7.2.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T0620119</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.-" evidence="11"/>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8175024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VDF0"/>
    <dbReference type="OrthoDB" id="289856at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140358">
        <property type="term" value="F:P-type transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006544">
        <property type="entry name" value="P-type_TPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047819">
        <property type="entry name" value="P5A-ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01657">
        <property type="entry name" value="P-ATPase-V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630:SF8">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12409">
        <property type="entry name" value="P5-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="387"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="417"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="857"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="900"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="931"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="974"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1006"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1045"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="domain" description="P5B-type ATPase N-terminal" evidence="13">
        <location>
            <begin position="50"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="12">
        <location>
            <begin position="253"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF12409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8175024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1082" mass="122469" checksum="2E99A6F6D1C0678D" modified="2022-10-12" version="1">MIKISDSINYQTTTQGVKDGLDISSSDLGVSSMEFLGEFDDQYKLIAIYYRINKLKNAFFIFLLLITGGLVYLFYRWFLSFRLAIRYSQCNFTQMTHLLINKTKNDIRIATDQLVEVQQNTCKLPNSSNARLFDYELSQFYIDCQTFQLNYLKNSFSHLTCAQIATATNLNEGSEIYGKNIMNVPIKSIPLLILDEILTPFNIFQIFALVVWAVDDYVLYAVLIFVLTFFQMVMQLREIRANLFKIRNMILFSTTVKVCQFDTIIDKCSIDLIPGDIVIVEGNTKISCDCILIDGSCVMNEAILTGESVPINKTALLKVNNLFRQKENDNSMLYCGTFCLRSYSNSNMPVKALVYQTGFQTLKGGLARSILFNENQSFSFQRDSLKYLFVLAFLGIIQSGISLYLDFTNDEAIGQAIINALELLTIIIPPALPTALAAGVSLAMNRLEKQKIQCIKPDKVNVAAKVNICAFDKTGTLTELGLDVVGFRPIKGMGFDKKVQIIECDEISIEGMATCHSLSLINNEVQGDPIDLNMFLQTGWKYTENSITFQDKSLTIIRRFEFQAELQRMSVIVSDHKLYCKGSPEMIKTICQKVPENYKTILNRYTSKGYRVIALAYKEIPKNLDSEIQSGKRELFESQLIFLGFLIFENKLKEQTSRTIKELKASNLKPLMVTGDNPLTAINIGQQCFILEQNQKIYLSQIKDQDIVWQEMSIQDQVINEGSSYSIDNIQSKTLTTEDILISSNSYQLCITGDVFEQLQYQYNQAINKEEILNLFSQIFIYARMKPNHKGDLMILLKQDKKNFIAFCGDGTNDTCALRQADVGLALSTEDASLAAPFTSSIFDVSSLITLIREGRACLVTCVECFKFMTLYSCIQSAMVLQCYFWNTDLSLYQYLYQDLWLIIPLAFTMDLTNAFPTLARYRPISDLISIPIISSVTVAALASIAMQVGLVQYLTTQDEFELDEPDEGAPGQINTILILLSNSEILAVAIAFTQGPPFRQQVYKNIAYIVTVTLGILGHIFVIFYPQGFQNLEIVVITSLRFEWIIIGFSLIIGVFIILYEKFVTQALVKKYSQEDVNLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VE23</accession>
    <name>A0A8S1VE23_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0600202</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000060">
        <property type="protein sequence ID" value="CAD8174052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VE23"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052788">
        <property type="entry name" value="RING-type_E3_ligase_ATL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45798:SF97">
        <property type="entry name" value="ALCOHOL-SENSITIVE RING FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45798">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL61-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="133"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="197"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8174052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="242" mass="29013" checksum="042DE9897E343101" modified="2022-10-12" version="1">MFNNSNLQFIITQFQFAIIQWHKNSTKQIEFSYFIQLNNIISYFCKFKSQLFTTLFIFIYQKVISFDSKNYWFFKSKIFRKAHFQESIMKSNLQIDYQCEQTQMNAYELIKLVLREEEEEEHDKDDDESSNKAIIILIFLCIGLLISGIIFCLCKYFYERIKQKRLAQVRQLNESQYQQQNIQLETLCLQNRELEDCPICLMPIPSILLVSTSCNHKFHQACLTLWLQVYKICPTCRTIISQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VJN0</accession>
    <name>A0A8S1VJN0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0670217</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000067">
        <property type="protein sequence ID" value="CAD8177454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VJN0"/>
    <dbReference type="OrthoDB" id="299358at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8177454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="236" mass="27682" checksum="D4D35920463C29BF" modified="2022-10-12" version="1">MKIANLISKLHKSIYQFSDINTKLASIIFTNVALQSNFDQKFPGAKQIFHFNEQQKESIVKTKKNQQEIIQFNDSNYLGLYVNKIYNNPINRATRFEYAYVEFANKDYWQHMYEIVGTMDPKSSTLIIRIKEDVLKECLECNLDTLDQHKGLFIQKIVNIGMITLFQITLKYEKTLQKEGITEFAFCCLYSKEDIFTYGQKTLLYKSKGSIHKQLGQRFMIHRPEAVIGEVYPKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VJS0</accession>
    <name>A0A8S1VJS0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0630194</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VJS0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8175692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="216" mass="25795" checksum="9CCF57E7F5911672" modified="2022-10-12" version="1">MSLQSRKSIKHEKFLQTILEQSNQFIYIYLVIQFFSLSSLYNSNSIQIKQPPLQLIIPQINQHRFKNLLILLQYFVLLGKFNELQFLILLKLSLLFLNILIIEGEVLSGQIYLYCVLGQTRRNKNCFFQNYMPYASYLINALFQKSTFFFFLIFFTLSTFEERVALEYFKSHLREYFCIILKSNISTKKLNQLNQLVWMAQKLIQQVSLLYTDNDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VKM0</accession>
    <name>A0A8S1VKM0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0690232</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Organophosphate:Pi antiporter (OPA) (TC 2.A.1.4) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VKM0"/>
    <dbReference type="OrthoDB" id="287797at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000849">
        <property type="entry name" value="Sugar_P_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43184">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY TRANSPORTER 16, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43184:SF12">
        <property type="entry name" value="SUGAR PHOSPHATE EXCHANGER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002808">
        <property type="entry name" value="Hexose_phosphate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="109"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="145"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="317"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="347"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="373"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="414"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="444"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8178508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="481" mass="54474" checksum="35D4AF911D680987" modified="2022-10-12" version="1">MFASSIAEVTKKRILMFVLCFFQYAFIHCCRSTWSYVSGRMVGEDYFTKQYLGYINFSFLFCYGFAISILGQFGDRMSLKVFILAGTFTSCLVFTLIGVMIQIEVKHNYVYLILQIINGISQSTAWCGVLSILNNWFVTDKKVILMGYFAACPNVGNILGDVYSGVLIGKDDLSLYAPIYLAGFSLFIMNVLDTFLLENAPSEDIKRQMVEEYEKKKQANQQQPQLMISMIEKKYEENLDQYLDYQRKSSRIQYPNGASQIVIKQENEQINQLNYFNAWFVPNVALYAFAFGCVKAVYYILGFWLPNYLDSKNVKDVAWITAMIDLGSIPGGILICLIGYYYNKRAVITVPSLWIGTIIMISVSFSGSFKHEIAGYMCLIFLTGLFIGGCYNNISTAMTVELSNQPELKNSKSATSTVVSVIMGYAAMFAAINQLIVPYVEKRLFVYCSALSIIGGLFLIPLIINEVKRNKQMSQSQLKTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VRE9</accession>
    <name>A0A8S1VRE9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cullin family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0710188</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cullin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000071">
        <property type="protein sequence ID" value="CAD8179311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VRE9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cullin family profile" evidence="2">
        <location>
            <begin position="163"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8179311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="240" mass="28906" checksum="45E7732E2A24F437" modified="2022-10-12" version="1">MQESGIYDYICKIKIKRMHQYSKKNNFNLNFYQKVYKLGKFHFKLEMDICSGMLSKQLLSINSTIKADNSQQSSSQTDEIKLFFKYIFKLRNEQLKYKSILNKKDRVQIIRLLVNNKQIKKMKLNSITLIYQDQWKKLKIFVLINWKKNLSQKFIQQIINHEKSPKILAIYIDIIIRRDEGRNEIETDKLLTQIFCLFQLLYQRDIFFSTLLRTIIQKIAQFLVQTSLTLKIEINQKQMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VVL3</accession>
    <name>A0A8S1VVL3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">COP9 signalosome complex subunit 3 N-terminal helical repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0770085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000077">
        <property type="protein sequence ID" value="CAD8181814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VVL3"/>
    <dbReference type="OrthoDB" id="304051at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055089">
        <property type="entry name" value="COP9_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22788">
        <property type="entry name" value="COP9_hel_rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="COP9 signalosome complex subunit 3 N-terminal helical repeats" evidence="1">
        <location>
            <begin position="42"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8181814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="335" mass="40125" checksum="16EFB817102B65AE" modified="2022-10-12" version="1">MYLEKDEYDIPNEQQIYASFQSDSDQLKLQYLASSQSEYENKHIVFLSKYFLEIDFKKISIQTINIQAICLSKFTQEQKTHNPNIITKILNHIKKQLQQQNFLNELHIAFLESCIMSYNYKIGYQFIKSKIFLKGSLEKKSQIIIEYFYLSGLIANAQKDFDEAFRCYKIAHKFHPTDVFTIEAQKMESLLCFRLGLELRNWSHPTNQLLVTNLQSILQEKEYILIKESNLDIQDKDINICLKEWSLQAQLYQFLKNEQELHSKVTFNLINNHFKIQDYEYLIDLLLQINKIHNMFLINEEKHYIEFNYNNLNYKEINSKLENRFDLLKSLNNTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VZE2</accession>
    <name>A0A8S1VZE2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0800210</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VZE2"/>
    <dbReference type="OrthoDB" id="284828at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8183348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="524" mass="61032" checksum="F5159F3A9E6CAB6F" modified="2022-10-12" version="1">MFTSESVQEHILYDKIYQYLWVKSETSSPPSVLIPDTVILIRSMPIYWYFTDKETGEVKKKMRKNVTKENIKETWLNQVGRSGVVGYLLHFIENLDLNYPPEKLKISGQIQIVYFDEKGFENFMNSNLELPFGILQRFVEAADDKNSQIQAFWSKSVTLFTKRVTKKSYLNKSMNIYERLCTFEGPEYLSEATQVKDFQSQRISEQIEKMINHLDAISFGKLNISQGTFYFKVDKQAKCWFLFCGSLKFDDDKHYKNYPKDLYTQSQIQIPKSVDGIMSVYSQRPLQLNKESKCIKCGNIDKENNFIDIPYHYLLDQNEESLPADQWPNEIKKEAKTVKVAGANAGTQALLNTITQVPLVFQKIHEKLNYTNFDQFKNSDGFLHKTMQVCLDCYIVLVAHQEKVQKNCVINKISQNIRIRKQSGGFTNAKQLKAYLRSEQKEQQKKISQIKTTESNTSKQTQKIRPIQQYINMRLQSLSPQQKYSIEIPSTADYLSFKTKSSNYDSNLKIRNLYNQSLQAKLNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W3P2</accession>
    <name>A0A8S1W3P2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0800169</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W3P2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8183277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="160" mass="19251" checksum="9C148C60C9955A0C" modified="2022-10-12" version="1">MNKKINSNMEFFIQFNQKNPVQFKQKIIAQVPQVLEQENYHYGLESQQKLWEEQMLQNLHIPEDQLKTNQDICEFMYHQLQQPQQSKFKDTKKNVSYTELNEHIENILFLKFVESFSPQINCDNQQEQPQQSQNNQQIIQMNKKNVPTDQIYENSPQSQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W5Z7</accession>
    <name>A0A8S1W5Z7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome biogenesis regulatory protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0820024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000082">
        <property type="protein sequence ID" value="CAD8183872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W5Z7"/>
    <dbReference type="OrthoDB" id="304496at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8183872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="159" mass="19093" checksum="EF8B9920352408E8" modified="2022-10-12" version="1">MTSNINILFDKPKLTLTALDTVEQYDFDIIVDKVSEQLKYVSQRLIEGQQESDLPYDKENIILPRQQTYIQKVKDSKFAKKKKNRMVWDENKKDWALRKLKKRDSVIPPIIEATKQDAYEDVFRKKEMEHELKKSKKQLRLVQRDKKKQKKIQTVKKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WA18</accession>
    <name>A0A8S1WA18_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0830215</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000083">
        <property type="protein sequence ID" value="CAD8184729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WA18"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13983">
        <property type="entry name" value="STKc_WNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000918">
        <property type="entry name" value="Kinase, Wnk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000427">
        <property type="entry name" value="Wnk protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050588">
        <property type="entry name" value="WNK_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13902">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="38"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8184729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="798" mass="93190" checksum="A8923E7E3C8F061C" modified="2022-10-12" version="1">MENNQIMFMNQDDQVYIFSDEDDQISQIIEQDSTGRFCKYNEEIGQGAYKSVFRGYDNQSGCEVAWNVFQLNSVPENERRRVRQEISILSSLKHDNIIKFIHSWHNKSKKEIVFITEIINGGSLKNYLRRITRPKLKVIKNWCRQILFGLEYLHRQNIIHRDLKCENLLIDTNNNELKIGDLGLSIQLQQSFTTSVLGTPEFMAPEIYQEHYDTKVDIYAFGMCLLEMVTGVKPFCECKGGTGQVIKKVIEQQKPQSIDAIQNDKIKAIILECLKPPEERPSATQLLQTHFNLQVTDNDNLPVPINEQFLLQLRDDSKNSSILKCNLSNKIMLTIGSSKFETNSEISIHKQTQLLGLVKKNLIIESEDETEQIFNKYYKDYICLKNDESLQDLEQRQEKELQMLKQLHLQQKQEYLKKTSNNVNVKSSSQPFQSSGFLSLGSYQNFFELQQDSICPKQIIESPCLERNSNNRLLKLEMITDNSSQQQSFNQYIIRSQQLTIQQNNQKYKINQFKYLSQENHYDWPDPTRNDLMYLSTIRNQNKLRSISQDYKYTTNLPKKTYYNKETFINKVDDIPIKNNKFETVRESLYIDDIAGTRPQIAKFKTTRETNPVEPIYRIPSYQGADIPQPNKFIRDTLNISDIQGTVPKIKIYNPRNVDPYTFIEGSKPKQLRHLIHPQEEQDMKRTSIRQVNPLQPFYKWGEEIIGPVEGSQPYNHCKDRNKLKSLSLYTKDITGAQSDTVKKIPKESQKITNNTQDIYRAQANTIQRGMQTKRQTNPLQPAYQLLGQSELYNLQFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WH96</accession>
    <name>A0A8S1WH96_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Insertion element IS150 protein InsJ-like helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0890122</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000089">
        <property type="protein sequence ID" value="CAD8187529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WH96"/>
    <dbReference type="OrthoDB" id="311540at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055247">
        <property type="entry name" value="InsJ-like_HTH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13518">
        <property type="entry name" value="HTH_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Insertion element IS150 protein InsJ-like helix-turn-helix" evidence="2">
        <location>
            <begin position="22"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8187529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="206" mass="24169" checksum="E5CB5309C0EF5561" modified="2022-10-12" version="1">MESQFDGSQTKKKYAIITPAVRMAFIKRVQSRQSTIKQAAQEFGIKFSTSKAILQTYKREGRVGKKKTRSRKSTLKNESKQEKQDEKQQPINSNDQQSLQKPQPIDITQMNFQQQQALQFQILQLSLSMNPYLFLQQNQYLQQSLQQIPNQQLFYYQWQMNNLRTQAQPQQFGKILDQRQMITPVHSSKLLDTKMDEIGATLKDFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WJF9</accession>
    <name>A0A8S1WJF9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0920101</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000092">
        <property type="protein sequence ID" value="CAD8188807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WJF9"/>
    <dbReference type="OrthoDB" id="306798at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8188807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="496" mass="58636" checksum="267DC8A461CB0687" modified="2022-10-12" version="1">MLNKFTLQFKDKQLEEKYQNYQLISNRLPLFKHLTLGLSVAGIIRFCQILISGDSKLWFIYVLFIIGVISLGTFVLIKKKSIRIALVIINHLIILTSLEIDNQCSPHYYYLRGASMMCIHLVILLQGEFIDAFFSIIIITTIRLLTIFLQDSIFPYPSMMIAIILIGYLLYIIYKNNLAFRSQFQLSCLDNQWDQVITTLIDDPYLLINFNESNLSFKFKKSNKFQFNCENELDLKLFLRNTKLIEGKNSLEQFLFKSIQDCIFENTNQQLKQQIQIIHDKKEFTLQYSIFLGVQPLILIQVLKFCKIQIIEDKKFIKLESSYQFKYKKLLSIVYKRLNKMMKKKSTFIIIHSMYKQILYFYLIELCEQSSQQIIYTFKLQSVLKKIKMIYNYRSIQIYNSISSNIMGNKSILCLILIDVIEQLTTHNIKISCSQLNENEYQIKIEGVINDKAQNVIYKRLKTQEWFFKKFTIDQSCIKLILWKDLPEIEGQGITK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WJK4</accession>
    <name>A0A8S1WJK4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0920121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000092">
        <property type="protein sequence ID" value="CAD8188847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WJK4"/>
    <dbReference type="OrthoDB" id="298589at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="430"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="437"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8188847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="559" mass="64932" checksum="9D76B75C1DD4065F" modified="2022-10-12" version="1">MKLSLSTEWLFFHLWKKNPDTNSSCPGVFVADTIIYRWAQPYFWYFTAKDGQILRKTKERIFVEQIEEIFQKTGDISALYLQSQQNKIIFEYFEKGEFVQFLHQREKALNALLQKFVEPKSSRNSMIKVSWSPQFCLLSRKTNNNDLKNNKIPIEDRLVTFEGPEHLSTTDSIASPILSADIEQICLNIVKHIQDVSGGNIQISRMVLFFKIDEKNRLWLLFSSGVKVRQKFINEETVSDFPKQKERIQSPIMILQTQLKIGQTSKFSVDTQGFVKQTGSSCNNCSLQGEMYELTVQQYIASYDQGIFDEETLIIRQKVAKNPVKKSDNEEDYYVEGSLNIPGLLLKIWGKMDIEKYKNLRNNMSFMNLKLQLCLDCYLKFTRIHVESKSEREAKQTIVTIPPLWQPSKTKIQNNQETLTLVSSTKQNKMYHTPAKQVQQPSFQQQQKQQSQYQSQQQLQQQQSQQSQLTIQQPQSSIVIPKLNLPSKDISERVIATTHHTDRQSNFPSKRVAKIYNNLQGVSLPHSQSSLNTTRSTYTHSQRIVSIQQLKEILQTEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WLK7</accession>
    <name>A0A8S1WLK7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mannosyltransferase</fullName>
            <ecNumber evidence="8">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0940106</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Endoplasmic reticulum membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the glycosyltransferase 22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WLK7"/>
    <dbReference type="OrthoDB" id="416834at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005599">
        <property type="entry name" value="GPI_mannosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760:SF4">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03901">
        <property type="entry name" value="Glyco_transf_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="2 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035774591" description="Mannosyltransferase" evidence="9">
        <location>
            <begin position="21"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="160"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="291"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="343"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8189597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="491" mass="58025" checksum="F5317FDCAAA0D00A" modified="2022-10-12" version="1">MYIFFFALRVLLAIIPQALPYADEMWQGPEIAHNDVFGYGWLTWEWTRDQPLRGPIYHSLFSLLYWILSKLNLDHPQIIAYGPRILQCAISAIYDIYLIKLLTLEGIRNKLYIILVNYTLWFSLNCFSKTLINTFETILFTLALYNWVQYLQTNHNKNNLLCRLLVIVNVMARQSSIIPWIFIWPYHLITAKTNLKGRFQILVINGITLLLLIILSISVDSLYHGKLTSTFYNFLEFNLLSGMSSFYGVHDRLWFITQGLPYVQLGWVISFIIGIYSYINKGIFSAQPLRLIYYMIFTILMLSLSAHKEDRFLLPLFPIIIYFICLGLEYLDKIKWKKIKKIIILLAVISNISFFVLMNTYQDVGALKTMSILRQRNATEVQFFTQCHRTPFYSFIHKNISMTFPDCSPTNDVTSLETYQLHQDSAKYIQQKLQQSKLPSHIVIYNYLMNDNVKKLLDKYDQIESIYHQYYTDTYFEDQKNIYINIYELHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WLV4</accession>
    <name>A0A8S1WLV4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Heat shock protein 70</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0940151</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WLV4"/>
    <dbReference type="OrthoDB" id="2401965at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11733">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000036">
        <property type="entry name" value="Chaperone protein DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000014">
        <property type="entry name" value="Chaperone protein DnaK HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000004">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000003">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00332">
        <property type="entry name" value="DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012725">
        <property type="entry name" value="Chaperone_DnaK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02350">
        <property type="entry name" value="prok_dnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="626"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="633"/>
            <end position="665"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8189687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="665" mass="72228" checksum="C0AB7C8FE5334873" modified="2022-10-12" version="1">MLSKLLRSTSKVNQHTVNAFSSKVMKGEYVIGIDLGTTNSCVSIMESGTPKVIENAEGMRTTPSVVAFTADGQRIVGAPAKRQAVTNPENTVYATKRLIGRRYDDPNVQKDIKHLSYSVVRAQNGDAWVSLKSGQTYSPSQIGAFVLIKMKETADAYIGKPQSKAVVTVPAYFNDSQRQATKDAGKIAGLDVLRIINEPTAAALAFGLEKKDNKIIAVYDLGGGTFDISILEINAGVFEVKATNGDTSCGGEDVDSILSNWISQEFKAQAGVDISKDKMAVQRVREAAEKAKIELSSTTQTDINLPYLTADASGPKHCNLKLTRAKLESLTEDFLKKTIKPTENCIKDSGVDKSKIDEVILVGGMSRMPKVQKLVQDLFNKPPNKSVNPDEAVSIGAAIQGGVLKGDVKELLLLDVTPLSLGIETLGGVFTKMIPRNTTIPTKKSQTYSTASDNQTVVSIRVFQGEREMAADNKLLGQFDLSGIPPAPRGVPQIDVTFDIDANGIVHVSAKDKATAKDHSITIQSSGGLSEAEIQDMINKAEKYKDEDKKRRELVDLKNEADGAIFNTEKSLNEHKAKLQPNEVQEIESAIQNLRVLLTENLTANDVQRLKDAVENVKNSAMKIGQAMYRNTGGASEQQQQQSSEQQGEQQQQQEGGENNNNKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WPL4</accession>
    <name>A0A8S1WPL4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0950156</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000095">
        <property type="protein sequence ID" value="CAD8190149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WPL4"/>
    <dbReference type="OrthoDB" id="10413893at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035827973" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8190149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="564" mass="68291" checksum="1DBBA9FBC50E0CAF" modified="2022-10-12" version="1">MYLIFIIELFLIQRSNSNTCQNISISPNQFYYSYYSNLLILEANDENFSNVQSINMEIVKKSFAIPFHQNLIAIQIINPFQMKNKIQDKLICQLFKVDEYSINCIESHSIFFDEKDEFKLVIQIQTQNMSQDYCDYMHFDETKQIFILLCHNYPMFKIYEVNIHNETSLLQQLNLTIQFQEKCKRAQYKLNDQTIIIAFHKCNHWMILLTDQSNKQIQILFDYQYILMKDVLMDFYILNEIQFCETTSTIFLYFIEQNRYASIQITRSKINIQQYFIYIASNLIQKLIINSKCSLTIPIIYNQEYSENTTNVKKSEFVLQNLYDFNDINYIQSYLFLKRNTELIVDITKYINQTYQIETTPLIFLEQLNLFYQINPTNKIIQFYRISSLKKILKPREKYIFLIDEEQQLIGNTDNIQCKKIDYKDKDLNNIFDIETTFNCKMKFNCKIEIIQQNSLQLFNFDFLKNRTNYLSIGIKRMIINKNTCIIKNLKYKFYYKIYDLFTFDQDHIIYEIDNYLFVYNCQRKRVLLQIVLLSQQVYDYFKYQYRQVSFLQNLNDLLIQNKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WRX9</accession>
    <name>A0A8S1WRX9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1020120</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000102">
        <property type="protein sequence ID" value="CAD8192708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WRX9"/>
    <dbReference type="OrthoDB" id="285389at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8192708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="341" mass="38526" checksum="026EFCCE5D0FDDEE" modified="2022-10-12" version="1">MSISLSDVKLFIVKNKEPIIINISQGLETIFHLETLAEQSPININLPLVDSKYPLKVRVNNKFAQFNPSELDSFLYYKLLLQNGQLVEGKISSNDDYALTFKIRQIANPMTNNSSMLSNSQSVKNKLYSIERGTSEYQSASSLRHSIPEVSFSQAQRFTKEYLNTTEYNYNLPGSIGSGRKASFGYGSKQIAHAYVQRNAEQNPPPNAYFLGNPIFFRKENKTNLPQDERWKNQNVVTLPGPASYETSNPVGTDKPAATIGIRFKPVTTFKDSVPAPNSYEVNNKLIEQSRFNKISFGYGQKIDFTKNNQTPGPGAYDQKSVFKRNTNQIIKNLTQLRHND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WT00</accession>
    <name>A0A8S1WT00_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA polymerase delta small subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1000065</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA polymerase delta/II small subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000100">
        <property type="protein sequence ID" value="CAD8191887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WT00"/>
    <dbReference type="OrthoDB" id="3763at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043625">
        <property type="term" value="C:delta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007185">
        <property type="entry name" value="DNA_pol_a/d/e_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040663">
        <property type="entry name" value="DNA_pol_D_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024826">
        <property type="entry name" value="DNA_pol_delta/II_ssu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416:SF0">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18018">
        <property type="entry name" value="DNA_pol_D_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04042">
        <property type="entry name" value="DNA_pol_E_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA polymerase delta subunit OB-fold" evidence="5">
        <location>
            <begin position="51"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase alpha/delta/epsilon subunit B" evidence="4">
        <location>
            <begin position="202"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="262"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF18018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8191887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="459" mass="52661" checksum="0BA9F2B6E6DF1452" modified="2022-10-12" version="1">MELENMAQPSFILNQLKQNQQPVSKSQKLELDIKQSNRFSLPSSNQNIKAQNSYIYCIRYLSMSKRFKIEHLKDSNFVEAIQNIQNLNGRVKVMGTIFKIMKLKPYYFQQYTEIKIADSIQNEEICYLEDKSGRIKLEFTNCQLNLPNNTSKTIKVEDLITGIVVMIEGQIVGNNIVNVQCINLPDFIDPPIYKPLNQTTYVCLMSGLNYDLENNTTKLRHLIQYLQGNLYFGYGNEVSSNISLIIIAGNLYGKQRDAKSCLTGSIKQLQDFKQLYNQLQENIKGVDELLNELANIIPVAIMPGQNEPVSQMFPQSPLQRAHFGESFENNTKIKFLTNPSEFNLGNIHFVGTSGQNIQDIKKQQNIKDQQDIDLIEMNLFYGHLAPTLHDTLIQQQQFTNDPFIIEQLPNIYFVGNMKKFGTKRLSNNLRIISIPQFSESGIICLVNLSTFECFSFEIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WWA9</accession>
    <name>A0A8S1WWA9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1010157</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000101">
        <property type="protein sequence ID" value="CAD8192439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8192439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="148" mass="17525" checksum="072450D8E470ADFA" modified="2022-10-12" version="1">MIKKFEDEQNKVKQEFNQNLTQKKQEIQEALNKPDQINKVNLEQEKQNKIELKKVSEGGKVVADTNNSGWCFCFCEQVIPKTGKIQFAFQILSGSYFMVGIGFRGIMRENPYLXQRLFISNTNLRIQFLDIQFFKQFLIMYLTNFYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WWE4</accession>
    <name>A0A8S1WWE4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1030025</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000103">
        <property type="protein sequence ID" value="CAD8192940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="409374at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035805160" description="EGF-like domain-containing protein" evidence="5">
        <location>
            <begin position="23"/>
            <end position="1448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1040"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1133"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1260"/>
            <end position="1280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1314"/>
            <end position="1333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1339"/>
            <end position="1359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1368"/>
            <end position="1390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1402"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="40"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="70"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="129"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="232"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="282"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="328"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="372"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="417"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="472"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="518"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="715"/>
            <end position="743"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8192940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1448" mass="165993" checksum="3E350E1F6BEE5CC1" modified="2022-10-12" version="1">MEQRKNKKLLVLILSICCLTLGSTLTCTEGQYISGSQCLDCSSGCKECISNTSCSLCKEGYMLSSYSCYSCGNGCQKCLGLSGSASRILYRSGGFRILATPTQTECITCYQGYYQQGSVCIACDKPCLACSSNSTNCTICVDSINQSTPTCQCNESYIMNTSTYQCTSQNIQQTCEDGYYLDITQQCQVCIFPCLKCTNQQECIECLPGLIQFETTCKCPFGQYEEIDKCVNCQLPCLSCQGSASKCLACAPQNQVVNEIFQCVCDYGYSLIEQNCELCQTPCLTCDYRVDYCTSCMYEFQVINDGKCVCKFGYLQNDNNECQQCQSPCESCENAINECLTCIDTNQYVNLNKQCICKEGFYLDFFNINCVPCHYTCQSCDNQSCLRCLDTTLQLEIGSSSCNCPIGYYYENTKCLQCQLQCSKCIDQSDKCLECADPNSTLIEYQCICNTGFGISKTNLQYCIPCKYPCLECSNDVNKCTSCFQISLIYLVNQECRCPQGYFMQDFDCIKCANQCQECQIDSDNCINCIDLSYEFMNNGCFCPYGYYVDTSLKCSQCQEPCSTCEYNYDFCLSCIDPLAELINNSCQCQLHSVQVFDNNNNLLQGCYKCNEIYLNCDICNQSICQLCLQGYNFNENQECLNTICGDSIKVDNEQCDDGNDIEFDGCFQCQCEFGWIQNQLGCQSICGDEIIVIEEQCDDGNDIQFDGCYQCKYDCNQYCNECIEGICMKCKEGFILEENQCIITCGDGILNIQTEQCDDYNNTPRDGCYNCYQEDGFICYYTNQLLFQSCERCSDYNCIQCVIQNQKQICQKCIDGYYIDSFQGCSQCDQICVECIIYPKNCIIFNSSFYQLKDCNQNLGLYYDYELQDCITKCGDGIINGQEQCDDLNINDFDGCNNKCQIESGYLFDQISLSFINEPLIQVQSQTSSNNDFSIYAEQFQDTINCTGTTINIELFNQSEYNYTINEQGLNCDISIQYFKTVEYLNLIHIFIKFKNHFTKRILDEIPIKEIIIVPKRQVYANEDQKEQGKTMAAVSKSLTSSIVVFAPLALLIGGFKFIWAILDIMSWMNNFYFLNVNYPENVRLIFQQAEWSNLINFPQLNIFNQPSDEYYFQAQIKFTEKDVDPLFINNIQIVIIFLLQVLITYIICFLFRKLMQTLFKKNIKVKNPSSTQIFQLQNNITNIFKQENQIKESKVNNQISIFEIPNKFKSIYISCIDCERSFLANLLKTLQISYLDILLAIVLQITNQQTSTNLIVKINIILAITFIGIVLLLIHLSYSISVQHHLKLDNHYFSRRYSCFYEDIKTNSKISMTYSFVNMLRKTIFIAATVLLYDFPIYQTSICFLSCFLNIILLFLCNPFHNRQQYILNFIPDFCICIIVGITIIFAFQDQYQIVQDDNIYILGWIIGVCIYLSILLQLIFLIKELIESIWKKIKELYLFLKNKYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WZ01</accession>
    <name>A0A8S1WZ01_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1090005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000109">
        <property type="protein sequence ID" value="CAD8195228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8195228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1304" mass="154578" checksum="69A316A531A084B8" modified="2022-10-12" version="1">MIIFFCIITIKACKVPYELQNLYPTKGERLEITMDKFISGNNLTYYLQTHQQGIMVQNLIKVKQVQKNKPSLNIDMKTNGKMPLQQLQKINISSNMECFSITYTEFYNIILDCQQNQTLFLFTLKDSQLIQIYTQNSTTPNQTKISTIVTNQQTYIIYGQFLIKNNKDLSWTILNLTVLYEQEKDSFINTHNLQENSSNFFASQRNSTNQTIFIQLQRNILSYVLSEDKMLILTKNMSFYYEIIKMAYYYSQDRCFYSDTLDIQIFYSKQQPILQSISENQYIQILISKQFIVIYNNCLINLIKYYFINLRQKIFNTVIYLDKFNEELFIFGQDIRIFKLVVLFLSLASNENNGNFTIQGLEVYDHFKIKLCKVLINYTYLNELDSNIYLKYQYEQQSYYNLLQFPFVYWITHLSGPLVQVIVNVSNPQLGNFSDKTLLNDNQQLQQKQKYYMVKAFSVNLFQECKTKNFSCIFVVAVSAQTIDIYQEGKLQPFSSTDIGQMKINVTQIEITFVVLNRQFYFIICLSFGQNIIYIYQYDSKLFKINNIILNTEPFQQFQLLTNAVVLLLNTNQIAITTFDNKSLIFFNFQIFQEISIDAIKLNLINIFINQQYQSRVLYINNKNSFIIGYIMEQFKFLLISIQLVIYEIQNLQVVNSLLIISWIPQSEKVVNFDVWNVYNATNSRNQGRMRSINYELDKMYINYYSDNLFYYVQIGLNLNVYNPNLPQHSSLYQRILFDGLYFTSIAIDQLAFLCFNQSFYLLSPILIQTFQNNTLHQNFFTLQIFNLTFYSQFNHSKSIHEGNNSVTIIYDYLYIDFNQTLINVNKFQYHVEIFRNNISFSGQALQFIITNKSQYVTYNLTNNIGNPQNQNQNTVYNQIIQYNKTQFFLMNNSAILNYSSNYSRNFGYYNACLASATQDLTIYAFCQDRQSHYQIISMDLSNLQNDIIPNQIINLTNFSYKQNVWIRIQNDQLYIWDGCQTIFHQFSQITVVRSIYLQKYLIWILDHSIIIYVYFTLIHKDKLFYKFVDRFSKVNNEIENFILLESITQQFYSFETIPRIVRKKKEIIIMISNPNFNLVVSFLLVINKYNIAASQILFQSIIQTSPPFEKHNFTISQLPQYDQGLLLFMYSNKSDINNQQHMIIYYNISDYSNPNHLTPLLFQGGYNFTLNQTLVNDFSFIAYNNSCGQILILTNNGSTLQLQLNIFSIKINLTDLRKNSTLNLKAYNFSNSGSAEIIVKNERSKAYLKVYPFISCLELQLLFYQHLHLFIIINKKIIKKKKLIRKMNYDQSKYYDYYYYYYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X3Q9</accession>
    <name>A0A8S1X3Q9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1080164</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000108">
        <property type="protein sequence ID" value="CAD8195179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X3Q9"/>
    <dbReference type="OrthoDB" id="299094at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="101"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8195179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="239" mass="28485" checksum="A8146A5D64A36A40" modified="2022-10-12" version="1">MKLNTQQLSEIKQELKKFSTAPIQKSYQLIAQVYKSQYELQSIIESQITKSFVRIGQKKYTDEDDEKYAKAIKICRKLVKQWKQVIISNDLSTNNEDNQDVQKSQQKQIQSKNNEPQIQKKFKNDEQKQETTIVQIKNQKNVKSNFKENQNSSSEYVQDDKRKKAIDGLTKYFYLQTKNEQIEWSKKIEQTIYDLYKHSVQIYMDKVKSIVRMIDRDSEFREKLLEGEFNLISEITNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X4C3</accession>
    <name>A0A8S1X4C3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1030124</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000103">
        <property type="protein sequence ID" value="CAD8193136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X4C3"/>
    <dbReference type="OrthoDB" id="296632at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12422">
        <property type="entry name" value="RRM2_PTBP1_hnRNPL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000566">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000977">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15592">
        <property type="entry name" value="MATRIN 3/NUCLEAR PROTEIN 220-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="13"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="193"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8193136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="360" mass="41795" checksum="6F724746A05029F0" modified="2022-10-12" version="1">MYNNSRDPPNQILLLILNSLPSSFPLNNQFLHEKFNQYGDINKILIFERGKTTKAFVEFHELKSAIQARRQLNGSNIQGGKMIIHFSRLKNLNLEIVDNSRGTDYTQASSNSQNSDSILNSRTDENIQLDLTNHISQTQSPRANSSPIRNEQINRLLESDDEDDLTIWKQAIPQNISEFHPEIQKLLQQRQSRLLRIINFDSKITGKMIYNVFSKFGNLEEILYQKSLSRAFIKYQTVNQAIIAKEYLNNIQFFDSQIRIYFEPLQSLQPTSFQDEYMIYYHDTQQFNTSPLSSNLLITCVQDPSEISEQIQMFAKAKEIKLGVNSIHLTMYSNADALKIIAVFSDYEFQNEKMNIILKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XBY3</accession>
    <name>A0A8S1XBY3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="8">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T1180065</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 12 8">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the PPP phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDO010000118">
        <property type="protein sequence ID" value="CAD8198312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XBY3"/>
    <dbReference type="OrthoDB" id="287303at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07414">
        <property type="entry name" value="MPP_PP1_PPKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000004">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031675">
        <property type="entry name" value="STPPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF300">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16891">
        <property type="entry name" value="STPPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="9">
        <location>
            <begin position="123"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8198312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="320" mass="36420" checksum="97EAFF354F2BCBD0" modified="2022-10-12" version="1">MSKDNKGEIDVDNIIERLLSVRGSKPGKNVNLTEAEVRGLCIKARDIFISQPILLELEAPLKICGDVHGQYFDLLRLFEYGGYPPESNYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRYTIKLWKTFTDCFNCLPVAALIDEKILCMHGGLSPELSNLEQIRRIMRPTDVPDTGLLCDLLWSDPDKDVQGWADNERGVSYVFSQEIVQVFLKKHELDLICRAHQVVEDGYEFFSKRQLVTLFSAPNYCGEFDNAGSMMTVDESLMCSFQILKPAEQGQGASQQNKAGSAKFVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XG44</accession>
    <name>A0A8S1XG44_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1230011</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000123">
        <property type="protein sequence ID" value="CAD8199914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XG44"/>
    <dbReference type="OrthoDB" id="288574at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035768155" evidence="2">
        <location>
            <begin position="17"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="322"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8199914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="407" mass="46606" checksum="99A2B9888C54B94F" modified="2022-10-12" version="1">MFLFYILLSFLHLVHSSEICKSLHVSTNLKKSKDDIISKGIASKQWNRKAKNPIIVPATQNIDYLYITSDDPNLSLENFQLDFDDHYFADSTTRLDHRPNGQHTEYAVEINYKCKQYGGTLINYEMTFFVPNCGDAQIYWKKFCGNPLTLREGFAVDMIYKNYRQEIIKNSEIINASYFDSDLDNYVFNVARDINVIKFILQMKQSNPTNDPIPEGFITDTATLKAIPEVNLTLPYVDIEMENVTKAWFEGDLVNGGLITEKENVLTLNFKCIGLGTSKIEITFPFQYFKDITLVIVKDCNYAFKVQNVINAKYQESDSITFAIYIIIFLVIIGLFYNLCIGRKKGLANIPMLITIIDMILCITCLQKTCVGKFIRKRFGLAGESFLPVDETDESFSSSQKQFYGSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XH98</accession>
    <name>A0A8S1XH98_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SEC7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1250108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000125">
        <property type="protein sequence ID" value="CAD8200740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="18431at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23022">
        <property type="entry name" value="zf-HIT_DDX59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015403">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032691">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HUS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046455">
        <property type="entry name" value="Sec7/BIG1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF375">
        <property type="entry name" value="LD29171P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20252">
        <property type="entry name" value="BIG2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09324">
        <property type="entry name" value="Sec7-like_HDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12783">
        <property type="entry name" value="Sec7-like_HUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="1">
        <location>
            <begin position="549"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8200740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1599" mass="187917" checksum="5A06DF7905EB202C" modified="2022-10-12" version="1">MNRQYVLPKYQEILQEYMEKVRKNIPKQYPSFKDFLNKMIDLVKYESLPKLGKHNVVFRLTINMRQTKLTQLALSTLFRLVSNGFIDGRIEDTSQEFYTDEFSELFNDSNYNKGEKRTIMDVLINSVTSCLFDKDEIIIQGLKLLVAFVLNPYCYVTSQNLTRIVRTIIFSYTRSRTQTVDRNTKNMLYQIINYTFQAVEGEVFTIQRNKRRMSIKEDSVQKLTQLVMLQLVDMVCLNRTSLNFCIQSDIQNERNQPAGYFGYCIMCRKSANLYCKDHRVPICSFICKKQHQEYVEQAQSTYSGAVKQFEENLDCALQLYDSLCNLLLNKTTLEQAKNQQIILECLLYILETPDFVLSKNEKFIKTTKERLCNQLLKYCLETEKTLYQFSFRIFQRLVNIMRKSIKHEMAIFINQIYLNILLSANSNVLHKQTALESLCSILERPKTGLEFYINYDCHTKHECLMSKIINTFYEIIVVSIYQKAEYQISTQQEILLKYLAIKALSYVIEGLNKIFDKFIITPSEEIGSPQMEDQNANVNDNTTVMYINPIEIQRQLKQEIMKGCQIFRKNPDKGIKYLLDAQIIQNDAKEIAKFFRENQQQVSKDAIGAFLGGHQQLNIKVLSEFTDMLKFKDLTVEQALRYYLDQFTLPGEAMQVDRVVQKFSDRYYKENPNSTFKSSGSIYTYCYLLVMLQTDLHNPSVAEKMKLIDFQKLARSINDGDDLPQDYLTQTYNSILKQPLAVREKEKSRVFMKESLTQSIRKKQDLFQREKEALLKQGSELIKTKQDSHETLYQIINQDMAYLIKPFLECIGKPSFEMFLFVFNNDQMEQASNQCIQGLVLFIKLCSFFSIPLQDYMNTLIKATRLNNQGQISNKHINLIKQILQTVPLIGNGLRENGWKSILKMISRLDEMRMIQQSKDNQDGQNISILPELLLESDLIDKIFVQSKQLDDEAIQEFINALCYMSKQEIYQTHPRLFCLQKLVEVCDYNMKRVSFVWTKMWNIVKDHINEVAVKEKKVAMFTVDSLKQLSIKFLQKDELYDFQFQRDVLKPFETIFLQSNLEVKEFILSCINHIVLNHKHNIRSGWRMIFGLIALGLKEENDKICKIAFQILSQIMEHNLDLLQDVFIDLIQTLKVLAGKNQEDMALASIDFTIKCLGYLSKQAQIIPKLNWNEFEEPEATVRNASTAVQLEKIWIPLLGVLSQLAGDKRNKIQAKSMEALFETLQQFGYAFSAEFWKMVFSTVLRPIFDEIQFTFQQNYTVANTNNDWFKDSCKKGFSLIINLMKRYFQKLRGLLPEFLKLFENCIQNQNVKLAKYSIHSVKNMTLKIGMMFNEEEWEQIIQFIDRMIRLTTPTKLSSFANQSLTSSRMRNMIEDCFTQCTSQLLIIQISIEILQHFNQKLNLQQLQTIENTFLYSYQFAVQFNSQIEQRYLIWKQGVLQDMKFLPGLLKQEREAYGCMIIIKKYKLKNHNILDIDSLIQPIDVFIKKHEMIRTNTQFPEELDQQMQKLKLQEIERETQNYRQLLDTYILPTLCELNVEHLKQNNQQLFLILLKTLSYSNDSIICKECIRCQKCLQQEKSNLDQQLLQLVQKLYDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XM28</accession>
    <name>A0A8S1XM28_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1280072</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000128">
        <property type="protein sequence ID" value="CAD8201552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XM28"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8201552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="97" mass="11582" checksum="64FAE71873AAB72A" modified="2022-10-12" version="1">MLFGELEGICKQKQLNLGITQEWLPDKKWLAKILFILDEKNEIFQIYYLLRGLDTLGEGKNSQIFFDDLINMWQKTKLRKLNRNCKISKLDCQKKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XQG8</accession>
    <name>A0A8S1XQG8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1340144</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000134">
        <property type="protein sequence ID" value="CAD8203726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XQG8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8203726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="265" mass="31903" checksum="9C6BACACE448706A" modified="2022-10-12" version="1">MICSNLEIVENNEKLTSKDLVQKMEAYCLEMQQCIESFFMRIDNLLDQIINDSFNSFNQNLEQSQQAMLHQQFILFPSLAIPRFWNQQFGNELIHSISSLLKFSIENQIKKNVEGLNLDNRAIQKENDMLRQRIRIKLEKLFQRISFFNKIQITRQINFRERKNSFGLQNVLLVWICDQPFPKIQFWYMLQRYYLKRLSRINFRDRSWGFKLKVNDIIKITFDLKQQIIKWENLKSNQSQTLTLETNYGLYSCFRLASSKVLLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XW21</accession>
    <name>A0A8S1XW21_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1400118</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000140">
        <property type="protein sequence ID" value="CAD8205370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XW21"/>
    <dbReference type="OrthoDB" id="306493at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="72"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8205370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="258" mass="29604" checksum="91F38E77CA015D32" modified="2022-10-12" version="1">MIVVVASNSQYVSFATIVRLMLIIIIYCKCVWIKNKSIKRGIIISLFKIICSIGFESYTAVKIENSMNYYTIYQAAIMAVLIVNSILLLLLMYFGWRIYVEINEKSFVQTSPDGFRMMEQLSAIQQQEQQEKSIKKEEIKNPTPDQPELEDKGSVNISINENMSPAMLKLKSSLSPSPGKYRSGKILDFLEKKISHAKNSQKELSNGFSREFNRKFNTDQIQQISLDFGDQEDISSVRNPIDVEIQNHNFSKDIKFIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XXL5</accession>
    <name>A0A8S1XXL5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1430053</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="412814at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015918">
        <property type="term" value="P:sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00433">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053958">
        <property type="entry name" value="HMGCR/SNAP/NPC1-like_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011356">
        <property type="entry name" value="Leucine_aapep/pepB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053956">
        <property type="entry name" value="NPC1_MLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000819">
        <property type="entry name" value="Peptidase_M17_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45727">
        <property type="entry name" value="NPC INTRACELLULAR CHOLESTEROL TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45727:SF9">
        <property type="entry name" value="SSD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22314">
        <property type="entry name" value="NPC1_MLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00883">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12349">
        <property type="entry name" value="Sterol-sensing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00631">
        <property type="entry name" value="CYTOSOL_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="296"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="324"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="401"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="468"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="689"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="715"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="750"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="795"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="6">
        <location>
            <begin position="264"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8206146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1428" mass="162916" checksum="A10065292A4172E0" modified="2022-10-12" version="1">MTGIMSLGLYNLTVLTDPQSLWVSSSSRTYQEQESSSKNYGPFYRTNQFILTYRNENWVNVFQKEGLQVIYFLQNIIRNRKVQIGGKNTTLDDLCYRPISAKGCYVPSPMDIWLQDPKLLEKDHDIQFTTLCTESIDVNQTNIPCSDKNGIPIILESVFGGINCENRQNDTQPCDHCYIQARTMAVTYLLKNDEFTKQNAELWEKDVWMDTLDALNKRDYKKLYDNYNKSLMVAPKEELLDQYKVAFMAERSVSDEINNETNQNAWIVVVSYLMMFAYIGLAIGQFPSKIYNGFTLGLGGIFIVAVSMISSIGLVSYFDIGLTMISLEVIPFLILAIGVDNMFIITHQFKKQKHQTIQERMGNTLEQVGPSITIAAICETLAFLVGSLTKMPALQSFCIQAAVGVFIDYFLQITMFVAFLTWDEQRKKHKRYDLIICKQDINYQLREDRKLIQTFFKKTYSKLLQKPFCIIITIIIFVALFVISCVGLTKIKVGLDEQVSMVEGSDLFNYMTLEKKYIEIGPLAYLILENLNYQDPHDLELVANLSNSLSRLNETVQPPIYSWVASFNLFTRESAEWTQACETQDIALYDLPTQLKRFLGVRINSPCCQRYGICGETFEADIVLNETGYVKTSRLRFQHRPIHNSAGYILSLEQTRQVIDKVVKQANLKEGQKVYPYSMPYVFYDQYSYIRAVAITNVLLALATIFFTMTLVQDVICALIVVLFVFLIAFNLIGTIWLTNIIFGGFVIEINAVSVVNLVTCIGLAVEFVAHIIIKFRLCQGQRWDRVREAMSTMGTSVFVGIACTKFIGVAVLGFAPSTLFKLYYFRMYILMVILGAFNVYDSQLILIGTSSSSNLFRIGRTLISNQVNQNQISYKQVRKQFEYHFECKWQKQRSYMTIILKYLIIMQKHILMETTPHIEIKHTTQLSTKEVYVLYYPSINQSTDPIVKALQFYFKSKELLKDINSQKSVKIYALNDDDPFEYVIMQNATNPRQDGANLARKASNKITKQKTEENTISVLLPHNYTINDIAEFTFGFQLANYRWTYQSIKEGGKSSQFPIHYLNLITPITYEVYLSEVEQKWNLKYLHHLVKPILRLRELVPTRPNIATTGYMKNYILNIVKEFGDKVTSTLIEGDELLTAGLRLIHAVGRGSIHKPFYSCLSYKGNADNDKYFALVGKGVVFDSGGLSIKSTSGMEQMYDDKGGACTVLETFRVIVELGLPINVVCSTAWVENSIGPDSFRVSDIIQSYKGITVEILNTDAEGRLILADAMSYAQDKYPIQEMIELSTLTGIIKSALGRYCGVFTNRKGCYQLLQKVEKITKEPFWILPLEDQHRELIKGSVADINNSSSGRVGASAAAAFLECFVEKKVRWMHWDIANVAVTDKNEGIYTKGGTGFGVMSLIYYMTWDIVSKELKEQKEENDDDDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XY33</accession>
    <name>A0A8S1XY33_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Hyaluronan/mRNA-binding protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1400097</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000140">
        <property type="protein sequence ID" value="CAD8205328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XY33"/>
    <dbReference type="OrthoDB" id="312043at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006861">
        <property type="entry name" value="HABP4_PAIRBP1-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04774">
        <property type="entry name" value="HABP4_PAI-RBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01233">
        <property type="entry name" value="HABP4_PAI-RBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hyaluronan/mRNA-binding protein" evidence="2">
        <location>
            <begin position="60"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="230"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8205328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="388" mass="45757" checksum="4CB519F6D84F9A73" modified="2022-10-12" version="1">MSNNLFNVLNFSDTEEAPEQPQKKNKKNNNKEQVIPVEQVVKENTQHHNPAPKTKGVTSEPHPKDRQSGTGRGKEQRKEGGGRNNWGNYKDDLKEEKYVAKERKSDDAPQEGKTEQTNQPVQPVQPPAPEKTLADYYQQRGANVDEILKQVEVKQQAPKKIDEEALKKEKLFVVKTREDEKREQEQKQKKTRQQQQTYRTELNTQAAEYLGFTSQPQEKNERRGERRQYEQPQQQQQQQQQEGEEQGGRGERRGERQDEQQEQGQQKGERQDRGEKQYRGDRLYKGDRQYKGDRQERGNRQERGDRQERGDRQERGDRQERGDKQEGGDRQDRGDRQYKGDRQNRDNNTRDQERGDKARRGGDRQEKKPQQRQQEQGIQLDEKDFPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y0T3</accession>
    <name>A0A8S1Y0T3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1480024</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000148">
        <property type="protein sequence ID" value="CAD8207611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y0T3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8207611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="66" mass="7699" checksum="D953EED42812A18F" modified="2022-10-12" version="1">MPFKALSLKFQTNLLIARQYILKLFYHNYFGNISRNSQGCIKSSFTKLKITSYINQSVSYSINLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y4N7</accession>
    <name>A0A8S1Y4N7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1520083</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000152">
        <property type="protein sequence ID" value="CAD8208885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y4N7"/>
    <dbReference type="OrthoDB" id="191686at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051480">
        <property type="term" value="P:regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051001">
        <property type="entry name" value="Calbindin_Ca-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19972">
        <property type="entry name" value="CALBINDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19972:SF10">
        <property type="entry name" value="CALBINDIN-32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="27"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="69"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="127"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="169"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8208885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="210" mass="24198" checksum="A7EAA16C669B2DE9" modified="2022-10-12" version="1">MRSTTRRSEQVITSQYVTPQGICDPEQAKAIAKKLFDLYDRDRNGIIDGQEVSPMLVDAYRGMSKAFNPTQADVDTFVQVLDVNRDGKITYQDVENYALRILAGIQPLPSYSIQLPPQRQYNRMVEERLEVARRLFKKFDVTQCGFLTRNEVPGLLRETYKQLNIEFEPTPQDVQAWMDMTDTDCDGKVALQDFEQSIIRSLQEQGISLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y7B5</accession>
    <name>A0A8S1Y7B5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1540099</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000154">
        <property type="protein sequence ID" value="CAD8209391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y7B5"/>
    <dbReference type="OrthoDB" id="287993at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45665">
        <property type="entry name" value="AQUAPORIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45665:SF9">
        <property type="entry name" value="AQUAPORIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8209391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="259" mass="28702" checksum="2272D6A58305588C" modified="2022-10-12" version="1">MQRFESDVNVEGESQAAYTRFESSPSVGQLSGTHQEKQTIIKSTLIASIGSFIFTYGILASAGNNMAILVIFFLSVLMTDRLTVFNPAVSFIDLLMARIGFQEFMFNVIGQVGGSLLGAMVCFFVLEDFTNAPYLDAKIGDNLIGSVEGELFGSILFMIVLGLQEDDYLKFSDDKLEHALVVTLGFGAARVLSSEGQSLFNPAFAFSLELFECIQDGSWGRFGFLWVFTCTPFMAAVVAIMFNSQIYKRFYDKKIQYGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YBP7</accession>
    <name>A0A8S1YBP7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1600032</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000160">
        <property type="protein sequence ID" value="CAD8210628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YBP7"/>
    <dbReference type="OrthoDB" id="288201at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8210628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="346" mass="41396" checksum="3CA003F657641BFF" modified="2022-10-12" version="1">MIEFAKLSKQEKEKYIKFLVDNKIEFQQSDQGFTASFKVQNKENVDPTRKSIEQIKRASPHQRTPTRSVSPLRRKFTREDAINLIQEIYSKKFDDASKQLKLKNVVNEDFGVYVMRFAQNKQIDIKDCMQIQDENVFLFKKFLQKEYEEEDLMYYLFLRAIVEKEIGQSIYQSRKSLDLQKQYLSICQVQSIISTVYENQVDEVQELLEYFRNHFDNNKISAFDFMALALEKYNNDRKPQDKKILINEQVINELQRFFYQEVEECTEQLFDQVKLIVNDLLQSIFRQDKRIWINRVQNAKPEDVLYLEGLQKQFKSLSANKQDIKLFSRKVLQTPQLSRCFGKLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YDH8</accession>
    <name>A0A8S1YDH8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1640101</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000164">
        <property type="protein sequence ID" value="CAD8211671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YDH8"/>
    <dbReference type="OrthoDB" id="294538at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8211671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="107" mass="12300" checksum="F25A044EE1B86FF5" modified="2022-10-12" version="1">MKKDSSTKRPYEKVSKIKRQNLVKLVFHQGIKIKHAAKHLKINYAAAKTVISQHRCNVILQNVQYKSNQRCGFTKINNINRSFSLISKLAGEIIKATDHTIQELKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YEF9</accession>
    <name>A0A8S1YEF9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1660079</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000166">
        <property type="protein sequence ID" value="CAD8212089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YEF9"/>
    <dbReference type="OrthoDB" id="299608at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="160"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="245"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="367"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="160"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="182"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8212089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="440" mass="51849" checksum="FD7820A323295534" modified="2022-10-12" version="1">MNQQIHFKFILQYLEFSSKFDQQQYELSIMLKRGTLAAQTIISKMQIRNPERTSNMCRLPIDQIAEFQQKMVNKDGVYIDYNIKFLFYMGGNQLGDCLINLAYFLNGEIDQDQKVKKPLTSTTDKRAALLFRVIRQPLQSQFESQEYPQVNVPMPVPTHTPISQQVPQTARNSSSRKMEFPNRTTRARSQSPPKEFKSTVLVTKTEEQTTSIKQPSDNLTQKFLQLGEKFSQEKLQQVVPPNEDNDNFKEITEMLTQQEEELQKQIEQLDNHIKIDSKLRKTNNFDYNNNTANFANVNNNNNQELQLLKQKYQELDEKYNKAQDEHQHLKRIIRQSGEQLKKQNQLTQSGYKQLSDNALNQSTVSNQEQFERTIEKKNQIIKDLSNQILELEKNTKATVIEQLRNQITRLEQQTQEKQDQWQIQEKIYKEKIFQLLQNTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YG44</accession>
    <name>A0A8S1YG44_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1690031</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000169">
        <property type="protein sequence ID" value="CAD8212561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YG44"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8212561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="228" mass="26598" checksum="CA8E9D6591066CC3" modified="2022-10-12" version="1">MIKAIQLQNHFTLIINYLLILTHFDVEQVKLNLYHICHVENVLQIQTNILQKLIQIGVKLGMNKLLQTLLLFNQIQEKFWRPYFDNNLIDECYNLKENCFGQWDYGDNSCFLDDIGSLCEGKGKYSSAQQYTCRSCENIRFNLLQIIGFSIWSLITIIPRVKGEITTQYYFLESPYLINIFTNYIQIISSLTTLKLASYLYCLNGVGTPIQRISSSMDSFLIIQFLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-02-22" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YKR0</accession>
    <name>A0A8S1YKR0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T2140002</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000214">
        <property type="protein sequence ID" value="CAD8214430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="2">
        <location>
            <begin position="219"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="196"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8214430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="403" mass="46290" checksum="75D5E8C252E14F56" modified="2022-10-12" version="1">MKQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKLNIELIIKQQLNFYLKDRQIEVKLKEKQEQLERFQQSNQKNEEIVKQLQLTLLNLQFSQQLTFSTTYKHSNCSVTQNGKVIENNEDSWNCCMCDQMIPKNGVILFAVKIIEMSYIMIGIDFRDIVQIKGYRNCYCIGGGTYNIYNNGHCYNHDQLDKNDKQIAFTFSTNDIIIVEVDIQKKYVKWTKQSTNQSFTLTIDTSKDLYPCVHLKGKCKIEILNQLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YPM5</accession>
    <name>A0A8S1YPM5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1820018</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000182">
        <property type="protein sequence ID" value="CAD8213622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YPM5"/>
    <dbReference type="OrthoDB" id="300641at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="138" mass="16080" checksum="802450ED04BA960E" modified="2022-10-12" version="1">MLICNNLEECNSCDFSKWFNNGAFIQSNKFCETCQSILKSDCIKLVSDIQRFIIFNNQCQYLPGYYQDILQFHPICGDLLVVDGEDRDDGNYNPFDDLMDAIIVNLDVMIFVKNVYMGFAIYAYNDCYRIKNNCFGIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YQ43</accession>
    <name>A0A8S1YQ43_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1810012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000181">
        <property type="protein sequence ID" value="CAD8213574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YQ43"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="176" mass="21206" checksum="E7FCCC1667B41AB6" modified="2022-10-12" version="1">MNGSHKKNEDLSQIFDQIKGVDERIYSLIIWMLGKQNSQGWMEFLSENENLRHLEEQISQSDNSIQEEKESRMDVMRNNIKLILYFIIKIQDHDFNKEDYSDEGCLEQRRDLIKSIRDQMEIIRFLKFLVHLTAYVQLQSVIDAAKSMRYINQFIFKKCYKTNTHSFHFLQDQKMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YQA2</accession>
    <name>A0A8S1YQA2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1980006</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000198">
        <property type="protein sequence ID" value="CAD8214162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YQA2"/>
    <dbReference type="OrthoDB" id="297088at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8214162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="248" mass="29350" checksum="CFC83348C52BB9DE" modified="2022-10-12" version="1">MNQQVQTKAASQLQFIVPQKAQLIFDVNQAYQAYSLPKIQYRQQLPELPDIPSKSIKQFDEKQEQTKIKNDNQIFKEWNVNYQEDILKRLANNKQLKAQQNTDLLDNLRSISKQPPKKKTRMHKTKFKIVKNEKNHPIVEQTMCQYISQNIQTQVPGSANNQDYDASAPFFIPTLEFAEQLNEEKQTFKKKYGFDDVTLRKNKLILEEVILLRLQKKEKIRQKNQIISQVKKDNDIDQQLKNKINIQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RZ67</accession>
    <name>A0A8S1RZ67_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0010264</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RZ67"/>
    <dbReference type="OrthoDB" id="283739at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="119"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8131744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="447" mass="53044" checksum="8C991D769771F672" modified="2022-10-12" version="1">MLQQKKQQIAHLNPILWQPLEKLQLTHLSQHLIYSGIIWIRSEDQQLHEEQVSLYADRLQIQQLFCNLSQTIIRRNIQTIKLVQGKNNTEIFFNRSADLDNWFNMLKRYTIQLNFSKDYKLNKRLGYQDPQTQIYQARCFANNINYTVKVIQKNNKFDPKQIQQEINILRRIKHKNIINLVEVYEDQYTIFLVFDNYFGTEMVYRFEDLLNSQEPQYARIIYKLLEILSCIHSLGIIHGDIKLENIYVKSDNLMDICLANFNQAEFIDNQEKGIKGSEGYQSPELLNGKPYDQSIDIYAVGIIFYYFIYQVMPFSESQEHNDNKSGQIEFPQVRNTSYAIDLLKQMLQVEPKQRIKPNVAMNHEWFIKMKVQEKLFKQKSFKDFTLPTILEKSDIFTQSPGQQPKRESIGVFEDEFLLDSLSDRMGLLKNAFNPSKMNHDIFQKKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S344</accession>
    <name>A0A8S1S344_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0020346</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S344"/>
    <dbReference type="OrthoDB" id="4033880at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03571">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000006">
        <property type="entry name" value="Clathrin interactor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276:SF45">
        <property type="entry name" value="CLATHRIN INTERACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276">
        <property type="entry name" value="EPSIN/ENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="2">
        <location>
            <begin position="14"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="279"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="190"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="218"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="294"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="331"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="358"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8133324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="559" mass="63896" checksum="DE33DD9DAB105230" modified="2022-10-12" version="1">MNLQSIKEAAQQLKDKLTKSPLEQTLSEATSNENWNTPTKLLQEVAEASYGYTSCDTIMKFIWKRLDSDNKEWRRILKTLNMIEYLTKNGAPRCVGEFRDNIYKIRSFSDFFLVEQGSDKGLSIRDKTKQLVDLLSNEKLIEEERESAKKIRERLAAAGGVGAIGSNTSYQGYGSSSYESNKPKNYGQESNSSQQTSYSSSYGGLESGGGLDKYKGQSNNNVNHNNNTTNTNNANTHNNNASANNNANISFNGIQWAAPQQQQATAAPFSEPVMKLAKPGEKWDVPGPKPQQPLPQQQQQQQQQQQQQQQQQQQQQKQPQQTDLFDIFDTPQQVPQQKPQTAQPLQPPPSQQQNNQNEWGQFQNATPISNPIPQVQTQQKAQTNLLPNDIFAPAVVQQQQTAVSFGQYQPPQQNNQQQFYQQSQQLLQQQQPQAQQSIQNLYPMQQQQNQNPSNLYQNYQINNYQQQQYQPKEQYDEFNFGEFVSATKPKSNNNQTDLLGMIDLKKEKEELEQKKQMPMAPQMNQQPQLYSQQVGLDGFQFNYQNKAPVYGQYQYQQSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S5H9</accession>
    <name>A0A8S1S5H9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Parkin co-regulated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0030196</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S5H9"/>
    <dbReference type="OrthoDB" id="429500at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019399">
        <property type="entry name" value="Parkin_co-regulated_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21207:SF2">
        <property type="entry name" value="PARKIN COREGULATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21207">
        <property type="entry name" value="PARKIN COREGULATED GENE PROTEIN PARK2 COREGULATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10274">
        <property type="entry name" value="ParcG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8134224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="315" mass="36298" checksum="EB45801D16B3C047" modified="2022-10-12" version="1">MLPKIQGQAVGRKENLASQQIVEDHLKNILKKQENQNYKAWKTPYAPKNPHSPFGDFPKEYLPKEKANQQKVIEPVHETSQNALITNKYQGTHQGTGGRTSSQLQKKEEYRPAPPKAGAFDQRTISVSDFRRYYDRGDLPIKVDHQGSVNRIIWKISPEQLDYHHYLPIFFDGLREKMDPYRFLAILGTYGLLENGGNKILPVIPQLIIPIKTNLNTRDPSIIEVQLKVLQKLVLSGEMVGEALVPYYRQLLPIMNLYKNRNSNLGDFIEYNQRKRVNIGDLIQETLELFEQTGGEDAYINIKYMIPTYESCILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S5Z7</accession>
    <name>A0A8S1S5Z7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0030433</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S5Z7"/>
    <dbReference type="OrthoDB" id="307059at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8134690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="274" mass="33432" checksum="7350EC652FB14A32" modified="2022-10-12" version="1">MQKVHIAAFTDDKVFINLLDKEIERHVKEYFEKDLHNNENRNLTSNKQKNKQYYSFSILHKNNVIVKDHKKIILKTFSNNTKFCDINQNQFNDIPLSERLNKRYNIVQRNCYFGVKPQTQQTQRSEFKSINEEIFQSVPNKTQKNNRMDMIQERIDICKEYLAKEQQRLKMVEEQIEKNQFLLEELVQELEIVNSNKTIFNSIKNLDQQVYKNELNCINYRKVTQELYEFIQQIQIKYFKKQQEFIYPFIQNFKNELNFMLHEYESQIQQNQVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S603</accession>
    <name>A0A8S1S603_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0040005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S603"/>
    <dbReference type="OrthoDB" id="10452639at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8135003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="164" mass="19887" checksum="1BDD0EA6DB579DB0" modified="2022-10-12" version="1">MKLNYDQFVKKYFPVQQITNLKESSSLIFVSKQGQIIKEQNFTENEWLLWREHFRQEIELQNILNEYEPNYFLKILDWAEEFQEQLHKVLIIYENQKDSQNLELYIKNNTISQEKLINIIIQILGIGMALQRRKIFHLNITPKNLIYVNGFIKLTDFVLFLKIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SA87</accession>
    <name>A0A8S1SA87_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Arrestin-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0050080</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SA87"/>
    <dbReference type="OrthoDB" id="297372at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF17">
        <property type="entry name" value="FI21816P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin-like N-terminal" evidence="2">
        <location>
            <begin position="13"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="3">
        <location>
            <begin position="187"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="368"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8136300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="393" mass="44584" checksum="94C9FA25F659EF7C" modified="2022-10-12" version="1">MGNQQGSDYGGVYIRTDKPFYFAGEIITGNIYLNIFKDGYPGGVVLLKVSGKEKCHWTESYSHTTTGADGKQEQHTEYVTYSGQTQFYQHRVAIWTFNTPCLPVGQYTFPFQFQLLSHLPGSYFEKGSDFQAKISYSVKAEIESYNKSYKNIKHSQPLVVREPLKQNMTALMGQMEVKASTWCCIDQGTSYIKCVFDKNHYLPGDTAYLWADLDNSNCKLDVKRIDARLINYLTLRDKTGREKVITRTIVSQQIQGIGAGVKAVENDRKQIQLILHNKNSQDGIRPSSNGQLVRSVYRLQVQALLSGCTCCNKHPTVDVPIEIVAPVPQVYNQPMAQPSNWNPQVFQPQIVQFTDSTMYLKDQNQQSNYNYPQVPLAPPQPPNQFGQMQQPMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-02-22" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SET8</accession>
    <name>A0A8S1SET8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0070074</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8138356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="180" mass="19446" checksum="C06D34A94009ED0A" modified="2022-10-12" version="1">MIKFSWXAQSTLTLCSDDACETPLNTNELVKGETIYLKQSITQEGFKGGKWKPMDAEITVVANGVTKSVKPKKLDKGQNGEQMYQLKLNFLGNKVTIGCEAQMGETTAARILQEAADDPSVVGSTSVSCIKETSSSTCPTEEQVLTYNSENCSGVSCDDDESSNAMIFKVVITMLGLLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SLI4</accession>
    <name>A0A8S1SLI4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0090276</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SLI4"/>
    <dbReference type="OrthoDB" id="434730at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005740">
        <property type="term" value="C:mitochondrial envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015227">
        <property type="term" value="F:O-acyl-L-carnitine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902603">
        <property type="term" value="P:carnitine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006839">
        <property type="term" value="P:mitochondrial transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF4">
        <property type="entry name" value="CONGESTED-LIKE TRACHEA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="5">
        <location>
            <begin position="2"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="5">
        <location>
            <begin position="87"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="5">
        <location>
            <begin position="186"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8140778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="272" mass="31259" checksum="D69230B88C8C589E" modified="2022-10-12" version="1">MKNIGIDLFVGIVSSCVNTMVGYPMDFIKTRMQLQQTKQSTMQSIIKIIQMEGIKAFVFAGSIYFTTYEQIRRQFEKYDNLPRNSYLPLYQTFLAGSTAGICSDLFAIPFEYTKIQSQKQEILKGQKVRGPLFILFETIRKQGIKQIYKGSLLQIIRDFVGCGSFFLAHSETLHFFTPEGKSRNEASQTGIFVASIAAGFGYWVISYPLDIIKTRYQVDNQNVLNIGKQIYLQGGILQFYKGFKITVLRSVFVNIFQLYTYENLRRACHKYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SLM2</accession>
    <name>A0A8S1SLM2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0090301</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SLM2"/>
    <dbReference type="OrthoDB" id="296718at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8140828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="269" mass="31991" checksum="60C5B444EB4D6498" modified="2022-10-12" version="1">MKELEFVELKIKEVIQQLKMKQQIESTIEKYLQQKNENTLTELEFQFVAQYLSQVSKKDNKEVKLDIEFKIQYESAQYIESLLQNVQQKLEVLNPYRNIQKIDSKVQINQFMQLLKNEILTVKPNEIIVTTLQAVRNCKLIGFMQPCINGIDQMQEQAMIFSIHQGYNLVEFIYQQQLKLNHNQLNVINNQYYIKITPVLLEQNKEYTIVLQSKAKVHLDQFYKPAIENPYLKFLKVPETEYKKIDQKLEIITNHGQFPILILETFVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SNT6</accession>
    <name>A0A8S1SNT6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PSI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0090431</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8141086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="287087at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PSI" evidence="2">
        <location>
            <begin position="42"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="2">
        <location>
            <begin position="366"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="2">
        <location>
            <begin position="579"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="2">
        <location>
            <begin position="657"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="2">
        <location>
            <begin position="1470"/>
            <end position="1517"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="2">
        <location>
            <begin position="1701"/>
            <end position="1741"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8141086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2401" mass="276547" checksum="7C4F64F21FCEBA18" modified="2022-10-12" version="1">MGCNPNLSQKLCIKQFGNMDGTTSECIFDTFCHTIYDKYTTRCSNKLSKSGCLSITNPDELCKWENGQCQNLSIEETKNINQVFQDLELSASVCSRISGYLIIHSTLLWDLLSYKSDLFTEAQNLVKVEAGITVDTTKIYEDQKLGDNYIDNNGDYKWYTTPTQLSITNLKINDYSRYGCIAQEILNESRFLTLFSLSGIIRGVNHIYCNYLSKHPTNPVKTVFTNYECQQITDIQLQDQQYIDQNQIVCQSMSGMVCQRYLNVNQKCQINYYKEYECYNIKTIQVQQQCNSLISLATHFDCSQVKGSACYLDYSNQKAECSQICVQYKTKINCENDTRCVWLGSNLFNLIDSSNSIFIVCAPINKCDQLGLNQDLCELMATPCYWDINLGRCKTIKNIEQMKCQNCNTIFCCTSIIMFDQFCIWHQNNCLNVKDQKLYSHYLSFPETTLMNYNLCVSQSGKYYYYNIEQQKCESSQVNLYSPCVDFEDQNYDVDDYVCNNGVNDRINKEYCLNLINSNTRWDYFKQKCLHVTNTHKSCNLMKLVNPNLCKEAIVLDNKLCLYDSQSSTCYYKDASVLHCDTKGINQVQCLSITNEYCAWMDNQCIYLQPSKINYYNCYELQKVSPSVCRLSIYQSIQCKYDEDSKSCIISYINISNCDQYANSYACRMSSSLCYYNNVIKQCQDSTPFLNQFNCNTEFISQSTCMGITKPGQYCFWGSINSDQTIKCRNHEKGFYSNCNTFDGYSNANICVNLTTNIALNLSDQQYCEVVDNVCTSLVTEINDIDCGYTRQMNVYRCMGYTTEQCYFQNQKCTILNGPQEYQDIILNQLLCQNSNMQICTKITTPGQVCYKQNTHKCISMYLSFGDNCENLSLLDMTIYNPQLCTHSTDACYYDKITNKCKIPELNDIFDCDQIGASQILCLLQTRGSCVFVNNKCSKLIDYTISCDYRNRMGCTYGELNCVWDQNNKQCKLSTELCPQSYEDIKSWRICGISSGICFAGRNGCSSEQVTVPCWLSLRYDLCISQPGYCQWIDDRCTDKEYDCEDSKTIQECTSHKTKTCVYDKGQCFEIINFQNYDCNDFQYTTLNFCWHYYPLCVYKDNTCQMINYNAKSDMIDEDFAQYQCSSLTQTQYGCALQRAMPCQFQDQIWFQYCSSIYTWQLNTCDYFDQTEVRSFLACESIENCGFKMTLKGEIFCHTIPLQCETLTPFCVNDIPGINCYLDNDVCLTYTGELTCSAVASYQVNRQLCISIMQNELNTEECYYQESTNKCKKRNTIEISDCTQYTELYQCTFVEESCLIVLDLDGVFQQCNISQNNTYDINLNYYGCIQLTGNLYYFDLNLQTCLIYDESKYNSIYICNQLNQQSCIQILNDLKCVWVNEKCEEYIRSYEQIDCQFKNRQTCINSIQSPCIWIGDNYECDISDNIGNQCNDGTYSNCQYSENYCKSISQLWNGVTCYDYSSNLPYSFIRCNRLGLSRSLCLENTAELTCFWDGQNCLEANLKTINCTDDVSFQTCEQILTYNQICKWDNGQCINKVNSQNCNDYITWTYCKQAIIPCSYNLGTNTCDQLIEVDQSCSDTLSYSACQKVINQFCSWINNTCQKWYRSKYSCENLANQWGCLNTSMFCTWNGKCENIGKDFQLVSCQDIPSGFNKVSCSKYSMEPCKYDDVTQKCNIKLTSEYTNSMQTDQSNFIQIRLIYECTQFQQKDDCIGSRIGYCQWQNNICINCSTSNICNNSTCFNNSIKQCLSISNVQCAWRNDQCVDWQTDENVTQTQLSLVTEICCTTLNMAIKYDQNLSSCIPLDLKLDNCNSKGLSKYACLKLINAPCIYYNNQCSLYQLLIKPNCSDYKDVTQSVCQMIQHLSCKYDSITNTCKDINTNSDTCNTLGLSLNGCTNIKTEACYWNINTKSCQNFIIKKGINQCDNQTQLNSLSCSQLNYHNNVCSYNVENRTCQEKFNKYFNCTYPGLNKMACVSLILEPCQYVDNKCQKIMSLKKSCLELSNVNEIACKMNTLDSCAYDPISNNCYFTLQQLPCNYHGLNYNNCVHQTNCKWSYDITQCICSIQQPKTQCQLTEINCKSNTNCIYDYDIDNCRLKNCNDLNECIGSINGKVCYKDAQQQCLNASQCEDIINLNNLSNCTDFVFNSSNCIQILNHCISASNLKELCPNSDCSNTACKYDNFQCRPLTCVDRNEEDCYNYSNCIYLDNQCQEISTCSTITDSTICIQTTINKFKCSWEMYGVYENTYHCTNTYCHLYNASPTLCNGNEINDYSCIMDQFLQCKTCESITDECTCLNSNGSCFYSNGKCNSILCQSFLTETTCTNSNKCYWSFQDKVCRKFCKQLILQQDCEALDYECHWNLYQYKCEQGIAVVPDLSVKINVQDIHGELISYLILILILII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SRQ5</accession>
    <name>A0A8S1SRQ5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0090477</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8141172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SRQ5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8141172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="96" mass="11514" checksum="9919E59B91A69C56" modified="2022-10-12" version="1">MRKVIQYKQIILINNKTYYHLMELLNQYKFKLQTSSKNRVGQLRLIKIEVFLYHLIIQIILLFSNFENGKQDHLRTFDLLEVLYVVYSLTTTGMNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1STG0</accession>
    <name>A0A8S1STG0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA mismatch repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0100310</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the post-replicative DNA mismatch repair system (MMR).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1STG0"/>
    <dbReference type="OrthoDB" id="10252754at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032301">
        <property type="term" value="C:MutSalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007861">
        <property type="entry name" value="DNA_mismatch_repair_MutS_clamp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF148">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05190">
        <property type="entry name" value="MutS_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="7">
        <location>
            <begin position="975"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="130"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="785"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR037677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8141794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1108" mass="128800" checksum="A2FD65ADCB728EAA" modified="2022-10-12" version="1">MKQTTLQRFLTSSQKKNDTTNLNNNKVSNVNSQVEEKKIEIQYIKPATSIKKEEQIKVIKEQQIENSQDKPQQLSRLKRNNIIDSDEEQVKKEFDQDELKIEKFSFVGSQEKNKEKKSLAVFIKQDNTKNITKSQQKQKKSEYDKSGKTLKQSTKLEEEEFDNSQENKQGNIDFKHPTDKINVYEEFDDVTPKWAAIGSSRDKSGKLHGTQDADPTTLLIPQNEFNKLTKCMQQFWKYKSENFDKIIFFKLGKFYELFYEDAYIGNKYLDLNWMGRKMHTGFPERAVHKYKALLLEYGYKVAIVDQIETPEQMNQRIAQNKKSGTGNTDKIVQRQISEILTKGTYLYEEGESQMNLDEKVLLVIRKKILSNTIEEYGIAIFERQTNKISLAFIENRDKNYESLKTLLLHMRPVETVIDQHNLPPTDPITKMISGSVIKSVISHLTTSKDNWDEKKALFRLEQYYQNGFPPAIKFYQNNQVVLQALNGLFTYLNQILILDRVLGCAKFKLYDEEFSLQQCMILDSQAIYHLEILQTTTLVEKKDYSLFGVINKTVTPGGHRLLRRWVCAPLYQVDQIRERQTMVSDISQFRKERDLFRQSIKQFPDFERRCSRIYEYSIKSESKAVFYENLSEQRLKEFKTLTKMLRLAQEEITLFGQLNINFRSERLKKMMTFEANGGLLPNVQEQLDEFEQYIIWEKEKDKDKEIPKPVTGVLESYDQSLAEVELVQQKLDDLLQKIKQTLFKNNSNIQYVHTKFRYEIEIPDELVEKSKPDDFEFTSSRQGYKRYITKEIKDLVTELEQAEELKKQQLTAFGNFIFKHFQSNQFVWDSLIKILNELDALCSLSVFGDTSDGKMTMPIFTTEKIKLVIKEGKHPCLTNINFVSNSIDMGEKLSKFQLLTGPNMGGKSTTLRMVCILAILAQLGCSVPCEEMELSPIDRIFTRIGAKDYLMEGKSTFYVELEETLLPLKYGTQNSLFITDELGRGTSTYDGVAIASAVMHYLIKKIQCRVLFATHFRILVEEAKLISEVTNVHMACYIQDGKVIFLYRLKEGACEASFGINVAKVVGIENSIITKAEDMANFFENKVQKNTEQTLQKFNQILKEYGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T1R7</accession>
    <name>A0A8S1T1R7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0150282</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T1R7"/>
    <dbReference type="OrthoDB" id="290840at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000093">
        <property type="entry name" value="NLI interacting factor-like phosphatase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="2">
        <location>
            <begin position="51"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8146313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="308" mass="36007" checksum="BCE9D462F61B52B8" modified="2022-10-12" version="1">MNTNDIIYQQQQEQQERKPNFLEKLCSCIYFFQKKTNKTNEKYQLGIDTPKSHSRKVCVLDLDETLVHSQFKADNGHDFSLDIIVQSQLFKVYVTVRPGVENFIDTLSEYFEIIMWTASLKEYADPVMDIIDPSRRALTRLYRESCTPIQGGLTKNLSKLGRNLKDVIIIDNSQMSFIFQQENGYLIKDFIADKNDNELETLLPFLIWLSQQNDVRPVSQLYRQYVMNESVQRKHSKRQILSQSMILNNQNSNKKTDSKAQRTHTVNHCDVEEIELRAEANLNSPNIEMKLNDQTNDESRETFEISNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T2Z1</accession>
    <name>A0A8S1T2Z1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0150082</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8145916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T2Z1"/>
    <dbReference type="OrthoDB" id="289682at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="112"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="145"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="178"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="300"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="10">
        <location>
            <begin position="557"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8145916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="608" mass="70171" checksum="E24A5F283CBC357A" modified="2022-10-12" version="1">MQSLNDGESHSTTLEPSKSFKSRAIFDTMEVSTKQFYMFDNDTGEIIDLRTSQIVEYDQIQFQKENAWQDFWKESQQKTLELIKACKLGQFSIVESIIQSSNFIDINAKDSNDQTCLHFACSSGNFQIAYHLIQKGSYVNEQDCDGYTPLMVSVKNNHLDLVDLLLYNKANVDRQDFQMNTPLHIALDNNCLEIIKLLLKYKADPYIQNADNINALQLISNTEQQQIFKQFGYEKQYITEKMISSNKETKESNSSHRSSISRIMKFLGQSVPTNSLQKQTYQARQSVVPIGGQNIGPDSFTYHKELGKGSFGIVYLVKKKDELNSLYAMKVLRKEKISQKLLPYIQTEKSILSVIDHPFIVKLHFAFQTQYKLFLVMDFCPGGDLTKLLDLKSKLPEHVAKMYTAEIILAIEALHQNKIMYRDLKPQNIIIDNKGHCMLTDFGLAKTDIENEDTKSFCGTPAYMAPEVVNKKLYNRSVDWYQLGTITHEMLFGMPPYYSHNREELFENIKNKQLKFPQNNLSKEALSFIQQLLERNPSKRLGAKKDSEEVKAHPWFQGINWDDVMNKKLPVPTPSIMNQANQTLQVNFDEDSQIQQRKTIDNWTFISD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T5T1</accession>
    <name>A0A8S1T5T1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0170158</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T5T1"/>
    <dbReference type="OrthoDB" id="306099at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000089">
        <property type="entry name" value="Cyclin, N-terminal domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0195">Cyclin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="3">
        <location>
            <begin position="127"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8147820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="363" mass="42048" checksum="862EE2BEBE33E906" modified="2022-10-12" version="1">MPLKIKQMKSKQLSSSAASESVSDFSSELSLRSGKVPTVTKLEELASAIEQKSSESENQEEVENPEEIHSPLEFKLDLSMIIQKNGKTEEFYNIVLQNYLGKQNKPIEYLFKDENGFDDEERIRFLNWLVEITYAYQMNYSTFFIVCSILDEFQKKTQNISKDNLHLTGVSCIYLASKFNDVIPLRLDQAIDISHNRLGRTKILQRELEIFQCLDHSLNFSNGFLFLTLLLRLYLSKKKDKENDIIEIAYFTLKLCCFDYELQSKYYQSEISAGCLSYAVMLVNSNDQGIVSEHITTSLLHLLKSANVVKDKQIVIVVKHIEGLIDKYFTDIQFKGKIQKLIEYNQTQTELLKSYLQIRNIDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TBA1</accession>
    <name>A0A8S1TBA1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0170255</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8148012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TBA1"/>
    <dbReference type="OrthoDB" id="302734at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="3">
        <location>
            <begin position="474"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="1">
        <location>
            <begin position="474"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="138"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="138"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8148012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="553" mass="65222" checksum="C74691D9D0209C61" modified="2022-10-12" version="1">MDYQMQPKELLKRLHQQLKSAVTLQSKIQNLNYLLSSTSDFLDTFAQSNSMKIITQYLKDALKSIQINEDLIDKILALLLKMNLSEEILKESEIVQPLLKIKDKKLFDNIRSNMIEQIQQKWSRCYIKFKPLLEEWKRNNNQKDKQEDRKKKGKRNQSSSSSESSNKKKKNRKKFVRFQRDDLLLNFKYFKRDDEPNQRGMTMEEVVQFQKQYAGELKRLNQINGAYNIKHRESLMEGQEHKMQLDNIQQMIEQIQFIKPAKLFLQSQVTYDTNYIETAIQQKRTESKMSAFYNKDSIPDQPGYNEISNTQSGLQPKIIHLDPPKREDLMYMVQVICNRGLKELEEKNKNANKKQVKGILKKQPDEQIKQKDVLNQAKQDLQPKMQQLVQMVEQKHQHSQESILTLLHDIIQKLKDIGEEKYIKNFKSILETKLNDKQTIMSENDRIKAQQDQQRLRLEQLQTINPAQALRLRAYKTKHCHNFHSSIGCTRGDNCNFIHDSRYPGRPAPTLQYPNFLNKTLYPQSNMAILVPPLLQQLNPAILLGRKKKYESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TBB7</accession>
    <name>A0A8S1TBB7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0190273</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="306704at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="241"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="265"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1066"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1237"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1342"/>
            <end position="1359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1563"/>
            <end position="1583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8149760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1627" mass="193203" checksum="BB4B963AEB41F7AC" modified="2022-10-12" version="1">MQDHFYCLTNSFRISSINLLIKSLDFNYDNTQIWSEIFFILMLAMQLLYLFCFIHLYCSYRVKVQNKQQRKQYKLFKQREQQNEINSIINVIAVSEIYITFATKIFKYLFHYPIIQLALRKILTQSNQYSNLNTYQILILIITYLVFILNILFYFLNESHDVQFSFKKLDYLSRFYSNKTKIQSLKIEILIILVSILDNLEINNIVLIIFEIILLIIILTLSYRYPQYQETLINQIQIQSFSLYLCIILCILIFAEGFHQDCSELILIIIPLVFEITNLIFIKQENKTQTLSYENKNFDYLMMTIYNQCQEALGRNGILLNEIKYRLPSNLNLYAFSTNHLINCQKINGCFCTCYKKQNDHFITRQQFKQYIKELIRFHYELKLLSLNKNNSQEYAKSQFYYILYISQVIKQPTKAFHEIIKLKNNMYSSLNLLDQMYTNSIELLVKSDFLKMIENKNITNQKYDCFKVFNYNKCVNQWKEQFSQVLQKQKQWYVTLLNRNLTQLIIDGKHLQNLISIQEKQLKYIFNTNPLSQQCHILIHLFYKYINFNKRRIEIPTIDSSIAFKFQNSINGILFHKEASLVYLTLLDTKGIIKNYTKTFRDAICTIDSEIMNNSINNFIPDIIAEVHDQYLDNFVERGRINIMKSDRRFLLVKNKLGFIFPIIAKVRLETDLGCDFGSSALILPTYQNYHYIMLNKYGLVEEISDKLYNEIILPILCIDLKSIKSLDCLKLIPKLIKSWKQLNQTNVLEQELKNPIQSYMVIPKKRKKQQILLSSQVKEKSTIYEYLKQQDQINYNYFDNFKQMYMFRITFKIVEFYTFQETIYCIEIQTIKPVRESQRIEIFEKLIEKSDYLNMSRRIIKLSPDKIEIKKKKCKKLEITNQKGSLGFISFQPEQQTIYNLETQKLIEDDYMIKQKEMQIRLTNPLYILEIEECQNQISHPNSQDKRLQDQQTFRMKSSVAHSFGALNHFCSSNSNGISQAFGSDQNKLIVHSSAILEALNENKESVKSKNSDELIGSIDNFLFDQDQMKSISSSQISDIKVKKTQLKHNLFDEQTKQHWTIRLFNLITFALLMIFNIYYYYVLKQENQIIEQALETYKISNNFQVQINNFILAFNYSNNNIFNYTLYMKFCASRFQNDISKFQYYSLNIEQNEIPYLQINEFENITNINLFYSLGYFSQYLLTQSNCQNQEYFLEFIARNFITIISSNSVLNQTVIKNGLDQFMRAQQSTKVSFYFTLISLCFAFIFYLLLLIIINKAKLKIVELFNTISKGQINFLIEQISIIGNFLEKIDFINEETTEQQFDQIKSKIATTPGIKIKTSQKRLSIKAKYNKSQENTAFTYFIYSLLLLFSYFIISSQYVGSLIYQSSFEQQTIYSFEQLLLFKSSQSYLLQQKSFQKLLIKYLQINKEDMLFLNKLEEALQYMEEQFDILQGFIQRLNDQKSFSNAIFTNTIKKNGCQAFQFKIANNIEDSQFFNEVICNNIDSLTSGLTISLVEILQNFQQFYTINKLNDMEFKSYLFNMDINLENDIINVIYTSHVLTLLHDFMANEQQQEISLNYLVHTLRFIFGIILYLILIIISDKNIRMYLHKELIKTKQLFLLIPLEILCENANVLQQLIIRNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TCD8</accession>
    <name>A0A8S1TCD8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tyrosine-protein phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0180171</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TCD8"/>
    <dbReference type="OrthoDB" id="10252009at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46653">
        <property type="entry name" value="SPECIFICITY PROTEIN PHOSPHATASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46653:SF1">
        <property type="entry name" value="SPECIFICITY PROTEIN PHOSPHATASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="2">
        <location>
            <begin position="7"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="3">
        <location>
            <begin position="73"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="325"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="325"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8148649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="411" mass="47846" checksum="D39B8B1DB5F22A4C" modified="2022-10-12" version="1">MEYDYIGAIKIKDGLFLGDQFASQDLEFIVTNKVSRIINCACKQIPNHWESIGIVYMSLPWIDNDTQVIFQQEELINQVIKFLDDALNNGESVIVHSIRGHNRSIAVLCVYFMKKYRWTLYKTLQFMHSRRPDLEIRANFFNQLLAIESKFQKNGWGAKTYNWEEVYSQGDPEEMVLRNTYLNAQPQGVAEFKDHDQKPKTQKLRFAEKITMYIPPYEKIIFNKKKQTQPIETKSCLTGKTLQYEQQIQQSPCPQTNLMSQTQPLQPIQPVHQRPSSQDVKRVVAKSDSSKLQQLPEATQSLKPKKQPNTYMDSGEQFFLKQQQKINQDSTQGVVQNDRRPQTAPNQLRAPRLQVTPYKKNTTGSQRQDLTIQPNTQFKPIGNRQRAHSPNAVYNANPYVQKQFKTKPWKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TCL5</accession>
    <name>A0A8S1TCL5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0220203</name>
        <name evidence="8" type="ORF">PPENT_87.1.T0220204</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8151949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8151951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TCL5"/>
    <dbReference type="OrthoDB" id="2017893at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF519">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE MPK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="5">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="6">
        <location>
            <begin position="15"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8151949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8151951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="299" mass="35302" checksum="172D043AEF8C624A" modified="2022-10-12" version="1">MKQYEHIILEDSENENIYRYFNSSFEFIEKGRQSGNVLVHCMAGISRSAALVAAYLMRKHNMSSKEALQQLERKRWQVYPNDGFVKQLLLLEKELNHQQQEIPYDWNAKIIYNQQNNIKSKTSSSDRLITKINKFNDNESISNEYQKIRKKYIDDEKSLDLLQKSTQYQLQQQRKTQFEILNNGINIQIQRLQQLSNSNSNRIINTLEPKQTIITENQQKKRALMDAINNFSKNSSVNKGIQVETSLYVPKKQEFSHKYQAATTRDYPLTNDFKAANNYQQKLDNLLNQFSKKSTYQYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TI80</accession>
    <name>A0A8S1TI80_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0210321</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TI80"/>
    <dbReference type="OrthoDB" id="308817at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8151374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="101" mass="11825" checksum="C864AB34B46C9336" modified="2022-10-12" version="1">MYKELEAAINNIDEQTEKQLQNTITKLVKAFSLCVENDKTDCKKYSTLFDPLKQSLDQEKQGLYKCIDENQITSQQCIQTFQKKSIQIIFQQLSKIEKEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TPU0</accession>
    <name>A0A8S1TPU0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0250069</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8153864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TPU0"/>
    <dbReference type="OrthoDB" id="283719at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8153864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="90" mass="10284" checksum="A387F393F212FB46" modified="2022-10-12" version="1">MGTCQTRKRKSNSQNTATTPVTQFSNKIISYSQNCIDTKPQPKFVPQRPSIVEYTQYDPTNPHRQFNVLINGVHFEVINSVEEDILGQLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TPV4</accession>
    <name>A0A8S1TPV4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0250171</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8154068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TPV4"/>
    <dbReference type="OrthoDB" id="304618at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="72"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8154068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="140" mass="16368" checksum="2CC8F4402EFCEED7" modified="2022-10-12" version="1">MINQECDTKIKQQAIKSALKKELPVEEQMFRRYDRKGRQIAFGSKYEVTIDEHVQYLQAILPVSTPRDRTKSPLSVPLSVRSPRSKSPLMDKAETATPIQNSQKQLEFTKKEEESLKILEIKYQKQKRLSKQSKQCCIIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TQ47</accession>
    <name>A0A8S1TQ47_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0250124</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8153974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TQ47"/>
    <dbReference type="OrthoDB" id="306508at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="93"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8153974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="174" mass="21094" checksum="708EB8538AA5A0B6" modified="2022-10-12" version="1">MNNNNYQFVGMSYTQGSSINQTQLSHSFARFPLQSSIMSVEEQSPQFAQLAQSQIYIQENSRFQEEENYKHLNQMIQQPQKQLEQQTGHKRMSSWQQQKSPQQQSQSKSLCASPLRQQYRQEEPQMYYHQPYQPCPCELEQLWQEFLQMRMMQRQFRRQTQQCCHHQRGHFMPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TQJ0</accession>
    <name>A0A8S1TQJ0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0250291</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8154278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TQJ0"/>
    <dbReference type="OrthoDB" id="290053at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003428">
        <property type="entry name" value="MAM33"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02330">
        <property type="entry name" value="MAM33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8154278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="264" mass="31031" checksum="0D41EDD8D758C36B" modified="2022-10-12" version="1">MNKHNKSTGIKYMAKTIFSLKRELDSQSKNMELIQQVTKEIKDFMVSKKYDIRSYHQLTQIYKYVQEQEFAITIFPRNKLVREKQKKVLALHELDRFDEYFNYTKLDSPALKHEEEARREDLVNSRLDTLNPLGLELFAQSPVQAVLTIQTPQIDQVMLLTILISSGNIEFKDVSVFDNINLRQEWHSKLLNIVEQYTDSYRADVLDLSDPFQTNIVKYLNAFGFEAEDAYYLELLAILSEQTNYLNWLNDLHNIVLVNQIHSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TT40</accession>
    <name>A0A8S1TT40_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0250221</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8154139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TT40"/>
    <dbReference type="OrthoDB" id="272512at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8154139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="40" mass="4923" checksum="7DD5D182DD1FB669" modified="2022-10-12" version="1">MFFKYQQSYMSVKEYSFGLITTNLKSIFVERENENNRSYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TUH2</accession>
    <name>A0A8S1TUH2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0270255</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TUH2"/>
    <dbReference type="OrthoDB" id="296164at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8155713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="59" mass="6914" checksum="357B473ABEFCD799" modified="2022-10-12" version="1">MGLCQSTQSTLKLPRIPAQKHIQINFDKIKQYEIQYTRQEQYSKKQSTEGFSPLNNVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TVF7</accession>
    <name>A0A8S1TVF7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0280180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8156200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TVF7"/>
    <dbReference type="OrthoDB" id="308740at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8156200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="258" mass="30146" checksum="2138F508A24CF439" modified="2022-10-12" version="1">MDQNNNQEVQNDIQYPFQAMLSEDQFHLFENVNVKLIGQNGKEIEFSPISGNLLITNERLCWYSLPQNNESQTNEQQQKDIQIEKTQENTSLVSNMNGKSFMFNYETLVSHGQQGDQFMCFLGSQEEEEMELEEEQEALIELIQQQNPAEIVNLGKGEYQIYFDLSKQSKETQNKINDIFTELATANVEMEDEGDEFMDEMITADNIDQDGNIIVKKQQQQEDQEIEDNDEDYEDVDDDDEEEEDNQFVNKKNNQEQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TVM6</accession>
    <name>A0A8S1TVM6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0270168</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TVM6"/>
    <dbReference type="OrthoDB" id="304904at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8155547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="167" mass="19200" checksum="AD503DE5DAC97F97" modified="2022-10-12" version="1">MSTDISNSPKPKTQSMSCSKSEIVSNFTEVIYRGPKSEIIQSKNIQDVDQNDIQLNKTQSGNYLNIIIIGLVILGLVAGLVIGYKIIKQGNVQDEEQMNVFVNIVSKSLLNNLEQHKFLIIDEQFKQLKKFYEKQLTNILWQKIVNHIQQQPNVILSQDKKKWILKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TXB3</accession>
    <name>A0A8S1TXB3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0290242</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TXB3"/>
    <dbReference type="OrthoDB" id="387093at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035743144" evidence="2">
        <location>
            <begin position="27"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="4">
        <location>
            <begin position="29"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="3">
        <location>
            <begin position="123"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8156908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="327" mass="37563" checksum="96E3A0C317EA26A8" modified="2022-10-12" version="1">MKNIIAATLTTLLIGFGFYHVNLDSASDFERWALKHGKSYYGDEKIYRQTIYYQNKQMIDQHNKRNDITYQMAENQFMTITNEEFIRLYLNTKSSEQLNDQDKVKRNNIHKSSEPINKENKKWPTDWRGLIKVHNQGSCPSSYAMAAVSVVEAAYAIEKGISLTLSAQQIMDCNYMFTGCDPNFDRNTAYEALLYIRQYGLYNGYQYPYTGQTQSCQINTGGPWKIANYWRGPYNDWYHFQSDLDKHPYEVAVDASKWQFYGSGIFSDCSSASSNTNYYALAVGYDSSNIWFVQASFGTSWGESGCIRLAPGNTCGILNDSHEVELY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U065</accession>
    <name>A0A8S1U065_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0310067</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8157918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U065"/>
    <dbReference type="OrthoDB" id="289646at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8157918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="132" mass="15446" checksum="090D3D0CE5A2C491" modified="2022-10-12" version="1">MGSSCKSIKNVKESNDFDIPEQEGPIRTVGDEPIPKKPYCCLSNELTEQSDNFGEIEIPKHTGMPQNQIFHNSIIYSREIVRSEQFQQNRKHRPKKRLNKVSPNTETHYEKQIYKQLRMNILSPDGIIRKIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U2U2</accession>
    <name>A0A8S1U2U2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0290276</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U2U2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8156976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="63" mass="7540" checksum="0E6B35034941B2A1" modified="2022-10-12" version="1">MGKDKQKKQNSKNNLKSKQKVPLKQYQIKKFQTSKQHNEKPQERNDVMKVQIQKKTISKKKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U3J6</accession>
    <name>A0A8S1U3J6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0320038</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8158582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U3J6"/>
    <dbReference type="OrthoDB" id="10069349at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23804">
        <property type="entry name" value="UBCc_UBE2S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000031">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="8">
        <location>
            <begin position="10"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8158582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="199" mass="22934" checksum="CF80D951ABEB2E74" modified="2022-10-12" version="1">MIQEPQINPQLGPIIAKQLQMIIKQPIDGVHVIFNEQDVFDIQADIDGPVDTPFQGGVFRCKLILPPQFPQMAPKGLFNTKIFHPNVSEKGEICVNTLKKDWNPNQWSLKNIFEVIKCLLIVPFPESSLNEEAGKLFMENYDEYFKRAKLLTNIYASKQDANILKGNNQIVIDEADKKNKVNQTQQKKENDQKKWLKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U5I1</accession>
    <name>A0A8S1U5I1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0330074</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U5I1"/>
    <dbReference type="OrthoDB" id="283627at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8159323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="55" mass="6288" checksum="D44A5BF31326DEB0" modified="2022-10-12" version="1">MNNCLSTPKTKQQKCPQAPKKQLNLTRIDDQSIRSVCRILFVEETKPEITLTQTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U7K9</accession>
    <name>A0A8S1U7K9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">KOW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0330082</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U7K9"/>
    <dbReference type="OrthoDB" id="28901at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032044">
        <property type="term" value="C:DSIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032784">
        <property type="term" value="P:regulation of DNA-templated transcription elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06081">
        <property type="entry name" value="KOW_Spt5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06084">
        <property type="entry name" value="KOW_Spt5_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09888">
        <property type="entry name" value="NGN_Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000053">
        <property type="entry name" value="Protein RNA-directed DNA methylation 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041973">
        <property type="entry name" value="KOW_Spt5_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041977">
        <property type="entry name" value="KOW_Spt5_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041978">
        <property type="entry name" value="KOW_Spt5_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005100">
        <property type="entry name" value="NGN-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039385">
        <property type="entry name" value="NGN_Euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039659">
        <property type="entry name" value="SPT5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11125">
        <property type="entry name" value="SUPPRESSOR OF TY 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11125:SF7">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23042">
        <property type="entry name" value="KOW1_SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23291">
        <property type="entry name" value="KOW4_SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23290">
        <property type="entry name" value="KOW5_SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03439">
        <property type="entry name" value="Spt5-NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="KOW" evidence="5">
        <location>
            <begin position="230"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="5">
        <location>
            <begin position="495"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="5">
        <location>
            <begin position="629"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="278"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="64"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="305"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8159339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="806" mass="93194" checksum="A51A79DBC8736DBE" modified="2022-10-12" version="1">MSAKNDDYRSDQDDKSQSSIDSIEDIQDSSESNQFKKSKNKKLLKKQKNQKKKRLNKQKFIDTEASETSENESDDDVKGEISKGQQEKYYKHDELKRKHHKDIVRDIEERYENGDVLNVEEIEKSEDEIEKPGLRDPKFWRVSCNKGKEQEAVTSIMFKHNHLIDTNPLEIVSVFSLKKFPAAIFIEAYFEQHVMRAIEGLVIVRQSPPELVESEQCPNMFQPPEVEQIDIEEGQWVRVRQHNIYSGDLARVMQVDQERKLIKLKVVPRQKLLMKSIEEDEENKKKNSKKNNSNADQSRFQMKEEEGEPPIEDNEPFQRGSKFKFLKRVKYLQEQKKFIRGPKIPIQMSKKSNHDDNDEPTSQFFYISIRDEWTSAKKDGFEIITLPVHQVLTGNIKPTVEDLQYFYPDVQDYRLILQKLHSSLKQVVEQKSKIQVGDYVTLNQNQDQTKASRYKVVQILEDQNILIVIKTIKNKTTFETKQTEYRLSINDAKLAFKLYQQVSIVSGPNIGLSGTIIKMDDLTAQISTEAGRIVDALISDLQSQKNVIKKMDIEENPDNGAEQQKTGFRRNDLVKFGLIDNDIGCILNISNNEVSILDLTNQIKTINKLAIRNSINTRNNVVKNMYCNDIRQQDLVVILDGFYKNNKATVLHVYRDYLFLFNGKFDNTQGVIIEKANNCGLVSSSKKPEPVVVNTQSQIPNEDWKNLRGQMVTIRKGQWQSYRGMVQEVTSRVATIQLSAKNLVVKVPLECIKSESSNSHLQVGKTPQHHPGMSTRVWDDLEGVQQSVMRGYQSPYITYQTPMRND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UBS4</accession>
    <name>A0A8S1UBS4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0370300</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8162298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UBS4"/>
    <dbReference type="OrthoDB" id="421226at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="350"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="396"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="430"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="579"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8162298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1074" mass="127342" checksum="196714A02423164C" modified="2022-10-12" version="1">MQLQTQIDTQQSHKVQQFMITDEQIGEIHHFVNNSLSDAYYSISQQNGQKQLEPIQLSNEEMRSQRISIDSPDARLILQASARQQKDESQHQLTLFQSWNIIKFIRRISERKRINTKFSQYQYDLINDKGSSFDLKLFKESSLHFKPIHRGLRRQNQSENLSKPSLTQQMKSYLIRQKQNIQDIFKRVEQYLQKIPLISPESRWKLIWDCFVAFSRLYFMYLIPIDLGWTEIPVIYGKLYPVSILALIILLLDYLISFNNSFYQFGQIANQRAIIAKHVITKSYGLESISLLILTIYLFNSDSNTINVLDNWADVFIALFYVQSRNIPKLIAQIEEVLNLSKPVSSMLELLKLILVLFFVLHCYSCLWFFVGEYSHNHSVQGSWLELYHIEHETFAVQYLFSFYYSTVTMFTIGYGDITPVSYIERIISIFYMMFCSIQLSYSVSTVGTILDQISAYEQEKMKKVHTINTYMQNKKIGYELQYQIREYLNYYWQSQSQVESQDEQNIINQLSQNLREQLMLQANSIILNECPLFQNHFSDQLKNKLVRQIKQTVIQPENIVEFDQIFPNLPSGQIFMCFIEHGEVQILIQNDQKDSIYSHQQVSVISKVGKGSSLGIYSFISGQKSIEKFRSIGFTKLLLLSRDDFLRILPDYPEDYERFRNIHDGLLFNQDYIKQMKCFSCQSSTHRVMNCPLLHYVSDREFIIKKHLFTKNHDRQHFKRNVKRFINQFHALIDQEEISDIAQSYSSCNSKWTEFYDEPPEEEEEPKSSQINKIAVIGTQSDLTQSPIQYHRPTARFNNQDIRTSKIDEIEAEDNKLKIIPIARGIKRRKSNILQVKQPEAKSSTQNTNSRRLRPSCTIEFSRFRQIQVLQKFRKIVQLVIKLNAMKKKKSGKKSLLNKFLNIQQQTQYLRLILEKVMIRINQIDQGKKEILKDVDVQNAFLLSIKIKMLISLQEEYFINKSLFQQMDTCKEFQYYQPQNNFSEILEKEEFYYQQGKLQPNLVELRKQLVKYLMYPQMYLEKYKYVKLQLVNLFTQRRFKDDNNRSQILTMSKRNKYRGKTFRIPKVNQILPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UBW6</accession>
    <name>A0A8S1UBW6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0370316</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8162328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UBW6"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8162328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="153" mass="18299" checksum="AE742032C69D6A84" modified="2022-10-12" version="1">MLKILLQIEREINNFNYLTQCAFALFRLLYSYLSVLLLQNLLDIYLQMMHQNLFIYHFDKQNNKILFFFNFYEGAFQSSPSYLQVYLSNFQTELHNLVANTLSQTGSVRTHEQRFQLLIVNIHLTQDSRLKWRTALQPFNVLPLQLEEVLTIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UC63</accession>
    <name>A0A8S1UC63_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RyR/IP3R Homology associated domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0360249</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="300855at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006816">
        <property type="term" value="P:calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013662">
        <property type="entry name" value="RIH_assoc-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015925">
        <property type="entry name" value="Ryanodine_IP3_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13715:SF99">
        <property type="entry name" value="INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR-LIKE PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13715">
        <property type="entry name" value="RYANODINE RECEPTOR AND IP3 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08454">
        <property type="entry name" value="RIH_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2396"/>
            <end position="2417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2500"/>
            <end position="2528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2540"/>
            <end position="2561"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2623"/>
            <end position="2642"/>
        </location>
    </feature>
    <feature type="domain" description="RyR/IP3R Homology associated" evidence="3">
        <location>
            <begin position="1867"/>
            <end position="1967"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2117"/>
            <end position="2147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8161563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2771" mass="330688" checksum="0CA54EC3C6B3D236" modified="2022-10-12" version="1">MDYNFQLTQVCSNDEILIQCNDMFLSSDGFYPPFIKIKSQIQIENMIFHIFPVSFDNDEIQILNEQEIIQYSQDIVLQLSKSNKYICVIVHNDKSILSLTNVIDRTSLFRIQPCYKSQIQNSNKVSFDDDIFLMYSPENITLQNLEQQSDLFVNINQNLLQTSDFILQATKQTTFKLKKHLKDNQSTSLCYGSLIKIKQIERDSYLELTTYENIQQNPHINNLHMTDVQTQYQRIFSKNKTVFWRSGERNQQNIDLLLYDIWRLYHPQKINQQIKQGDQFYLQNLSGNFYMNGDQICYNYQDASLYSYIEEESFNNKIFYILWNNQYLSYKIFNQNNFNQIQDKQLIEYKSKKSKLDLLQYDLISNVDFIHQNWLENIVQLIQQINLKYQLHNDQINLFIKNQSINDGKFDELFFQLQQLEIALNDLIIFCVADEDKPKIIDKNAIYYLKMNQYNQDLLRKKVIVQSLIEFIEIVKWQYEYTQIIEQNKIQLNNDIPIEDQNLQFLFQLDTILNQIFLFFEAFSSNNDYNSQIIFLAYFKLKQYLGYNVGAFYCLIRLFENNQNLIPVAGIMPFIGESEKEYDQSLIKRSTRINTILTKQQNYIKSQTLIDEIFERANYCLQILNKRFWGNIMKILSVLCLQNDKACFNNQINIGKHIIQGPKILLQFVIKEDNLLYIQIDENLHELNEIYVQSSKINRDFMKDIIECKNFLDSQLNLIGLLCQERNYENIQFYRTKLPQKILINYISYSGNAESLRCRLIIILMNLYVDCYPYQPKQIPKLIFNLNKLYGLQSNGFNAESSQLELQEFKSKPLLKQLTRQFTINDTKTIEKLLTITQELVKLNLIKENQINLSLSVCKTLQMFYKLYTFNLPQNKEFHEKYFDVNLLIQAYTLLPQSLEQNQPLQEQVQQYQQFQEQKEIFNQMSIILIDFLQLYFQQKETKILTSIIKTSQEFDYLDFSQQYNYNRYEKIKQNTFLLLKHKQSQTETQLKAFNLMESLYQPKRNIIKRLQKCLFLENQKEVTYYQYIKQMILQLKELESADGKQQLYQNCINSLINLNKQLINSDNLLLLQKILRILNFDYILLDFLESYQQICYFITQKFIKSNNDNRYILEINNEEEQILQFCTQSFIILKYYCNGNYLNKLRILSKFNQFDDFLKCIDLGQYDLLQEIYSESYENIESASSRIIYLLLDNLNLGSLKVLKSLIIQQNISSSKNLFEIIQQIQNSKKFKQQLGFQDSILENILQQNKYQTFQKQFQIGNLPYIFQIQGIQFILYLLKYSNIQGCKVLADIFPLQQIIKYCIQLKDIFRSTFEDLNIAILNTILKTQLIKLFNELLNYKEIIKYEDEIQQNIFQFIEKEMKIFESKVVVLDYQMKQTNDIQKIYDYINTNFKFKIQKKSKVNQQIFQQIQSGSENESDNDLKEDEQLLECQKQMAYFQLQFNFDCLYFNNYAILLLNVFLNLAQLIQEQIENIEESQSKTGNRASSSFARQMSISQAFADSIVNDMSQSVKLKPFNNLKIKVDSFLKQIEEYLKLNLSKWEILENGHFLIEKSQQLKIIFLRKLEFQNFNLDQIEQKKKDQIIDPYCSPIYCNDKRIHELILLKNKSNNKFIDKQINSFFINERILANENFDMYFQIYKQQLLMNDDYEIINENFSNIILPLINCFQSSMNNLNKNYKFIKKILKFLWHMLDIHTYIRDDYNQTVRIYEMNKKGKYHSKSEQILQIKAIKAFQNLLVNQGFFETFLQFCNQYEKIKIVQQSELHLINKKKKEKLLYQFLLTSIRLVRGIESANQIAQQKFMQAILKTKSNYMLKIFSNIFERYQQQDEILKLRSLIINDINRVYFAYEFNEIHRLEQKQNVMKLGILQVQMKLLQLLAEDHFRPFQDFFREQIGFAQNYDIIQHLMNILLNFFNQESCQALSQDDYILILNCFDCLKENIQGPCPQNQEKLCQPQFLRLCCKLLGFDDSQIKFLSLYQRFNKIDQALLNQEKERSRLQTQEQLRKNNYIQDKTTIQQDIIQYYIQYPIYCKQLVTSFRKSNSLNYRLSMLSEIKYRCATTLESLTDNEQNESDILKRISMVINEKILIHNITIQYEKYKILHGSGKNYTNLVFSHLLGEDAKNIEQENEKKKNEQKNKVQIQDNKSYWYQVEQEVTIDSAVKDFWDSFIIETGFSLFNLLAQVYQMKDQDILERIYEQIDNTKQQKKKYKSIIFYNLYKAIFSASNLTKNINQLQKSTSLNDKIFNPQKFKKAIGEYEAQQKKLGYRALRFFATRTGSIEILLPSKQLKKVMFPLVPHCFALNNVIKEKFASDIDRLSQQRKVESVLSNSSHIIQKLKTEYKFQALYKRNFIINLLASNQKLWKQIIFLVIVLENLMILFSSDSNLQKIFGDILFGLTITQIILQFLCFFIFIVKKLPYLKIKSQEIVFEKQINRIRKREIKYKYLDNSQSIVLQEDFVLEKTDAFLLEQNKWRRLYFNITDSLTIYKCILLDYEMIYFLIFTTLAFIGLYINIVLALLLLDVFWRFPTLTSIVNAIWRPSGSILLVLSLYIIMQYYYSLTVYFYYSDEYSPYCQNLLQCFSFILDVTFKTDGGSVGYVSSSDVYADENYRYSILNFWEFVYVFVVISLLYSIITGIIIDSFGVLRDEAEELDNDIKGFCLICGIDRGTLEKKAKHKKGFRFHVKYEHCVWNYIFYISYLEDKKKADYNGIESYVDSDLKKESINWFPINKSLSIPDEEEEQEELSKFKQTLDDRLIEISNKIDQLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UGZ2</accession>
    <name>A0A8S1UGZ2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L41</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0400227</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="4">Component of the large ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the eukaryotic ribosomal protein eS32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8164110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UGZ2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007836">
        <property type="entry name" value="Ribosomal_eS32"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05162">
        <property type="entry name" value="Ribosomal_L41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU368055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8164110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="38" mass="4939" checksum="7B98960043AD5AF9" modified="2022-10-12" version="1">MREKWRKKRMRRLKRKRQCDKYINIQSNIFLIQFLSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UQ75</accession>
    <name>A0A8S1UQ75_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0430189</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8165912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UQ75"/>
    <dbReference type="OrthoDB" id="294901at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004694">
        <property type="term" value="F:eukaryotic translation initiation factor 2alpha kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001126">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000818">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF136">
        <property type="entry name" value="EIF-2-ALPHA KINASE GCN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0652">Protein synthesis inhibitor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="195"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8165912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="492" mass="58711" checksum="5A045FAC1E58392E" modified="2022-10-12" version="1">MSSKFYIEEVFDEMQNEKVDIPNTDKNKIMLTLEENLKEKNDFLLSEDFNQFQQLLVISLITINFSSDDPFFRHITMVVKAIKKAIKKRLTQDTNYFQSIQEYKNQQSTLFKIFYQDINIGFITSTLQIKSTAFIFINKAIADSFMNKKISIEQKNERARLTLSFFNINIQKELNVQQDFKNQFLRNSKFKNEFIVLNEEFDQQKQSTTYHVYKPMDDQIYFVKRIQLQGIPINVTFQDIFNTKSSNQDYLTIQEARILQRISHPNIIRHYDWWLESKNQQLFLYTQIEYCIYPGYISQTKNLLSYSYFYMNPMPLEQKSKLIIEIICQVISGLEYLQQKQIIHGDLKPESIMVTKSITGDIQVMLADLNHSYSYFEDMQKQFKQDQAQYKQQSLQALGVLIMHLILTFPGEPTLRNNYITKFSNNDIEDSLSEFDKWAQKSVKNRHKEFSFFLYKNFMVLAKSLLKKQKFNELHEVRTFIQNNKQFDHLQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UQW4</accession>
    <name>A0A8S1UQW4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0460186</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8167015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UQW4"/>
    <dbReference type="OrthoDB" id="293710at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="206"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="144"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="216"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8167015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="431" mass="51025" checksum="082CB7CDB3E5EF96" modified="2022-10-12" version="1">MRPSFGKKVKQDDSDNKFREKVYRSMYPSPCSSYDPNKWLQNNDISWLSHSLEQDIDKILQIAAPQPIKPQNNRRASANDIREEFDDKIGKQKRNTWYVDKQHEDQPKNSKLKINMSSIICEFPIQKVSRQSISDQPKTPNCQKSFMEAIIQRYQQQNEQQQDDQFEKEKKYLFQSDKKQQIIEPEIYDNQIDDNLLNEEEQQNISITEQNPIQQKNKKQTKPKKVGRKKKSIMPAPKRKYKQTKKQERIQELPQPAQTELYQEKTKYIRPSQTIDQPIFNTKQCWARMQFRKEAKKYEKYQTQEFKDLLHSLPGCYIHKCEGDLRIDKIRPMQNAKGKFFQSFEMWINPLVLIGPIKMNNSVKLHVRFKKGYSGQVYCDSALQETLPLNQVDGKTFHKQKLMLANNSDHVIKLVCCLVNITIRGPSKRCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UR58</accession>
    <name>A0A8S1UR58_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0440066</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UR58"/>
    <dbReference type="OrthoDB" id="2019833at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SPRY" evidence="2">
        <location>
            <begin position="653"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="38"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="439"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="516"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8166282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="762" mass="90297" checksum="F06EB40DF304E665" modified="2022-10-12" version="1">MEQSNMKICSKHKLEIVLVDLEANVAQEDRYLCVRCIIERMNKKKINMIDEAKNLIKEMKMTEQNQKIEDNKKRLDNFKIVGQLLRDLKQNIDSIFDKIFNNIQSKLSQIQSDIENIESQTIIFNFEDDIERLSQFQEQQIHSQSIQAIEGDHKFIEQIQKQLSSFINSQQYNKLMNDQIIFVNENVAQDLQTNEQPNEMIKAQDYKTPALKLICEEHTKEIIMFNLNVNSNQLTNYQRWPVQNVFHNILHHTLLQKSLLANGINIKRKIKLIVIKSKKLGVQYIKLQNDQIFDELTQSLINQQQSIEHMNQNQINGKSLYQLTSNQINEILIQLSQIDKFKSHKDQKSNLEKIDKIFYSDLRSRLEHLIQYQQINAQRINYLLMNDNKVIHEIDNLLKSTQQEDVGNKEESADILQIIEKARQIMEQSFNFQFSQNVFDENIGQYKKAKEEIQNLRQILSFKIEKYQGFQQIFNELNNYSEKIDKDYLKFQQFLEFDQIIIEIKLNQYQKEIYVLTSLNEKMKIEMEQQIKLKNEDNKKLKEQIESIQKGNLEQKSQFENELRILYQTYQQINQENLELKNQIKVKDDNFKELKAKSSQQQMQLTFSQTNKHSTCQVTQNGKMVQNGKYCLCDKVIPKNGVTLFAFKMIELSDRCYIGIGMREIIQKNNYEGNVTYGQGSYTICSNQYCYSHHEKDKDAKNLSFDFTVNDIIMIEVDIQNKYIKWTKKKYNQSFALSIDTTYDLYPCVWVMSGSIIEIQDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UR78</accession>
    <name>A0A8S1UR78_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0460022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8166703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UR78"/>
    <dbReference type="OrthoDB" id="293935at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8166703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="234" mass="27183" checksum="EA8BA315672AA9CB" modified="2022-10-12" version="1">MSYDISQQFIEFRKQIMNKTLTTPATNTSKIQTISTPSKKQEFSVDILKQSICKNVKRLKVKSAISNIPLSPQKQKPTSPIKKQPYKSPLRLKQNEQIRSPNKQLYSSTSKSQQSQINTFQLPQTYRQSDYIDQQSQIKLYSSKYQKIMNEEQGLYYSQLQTKLDQLSSIIGNQDDRQRNLLEIDNMISQSKQINAKSRVQKHLDSLQFESKALQSDLNTIKTKISRFSDFTDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1URC2</accession>
    <name>A0A8S1URC2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0460275</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8167191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1URC2"/>
    <dbReference type="OrthoDB" id="312927at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013694">
        <property type="entry name" value="VIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45737">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45737:SF6">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08487">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13768">
        <property type="entry name" value="VWA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51468">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VIT" evidence="2">
        <location>
            <begin position="23"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="1">
        <location>
            <begin position="340"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8167191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="867" mass="100912" checksum="69E92AAEE302C7CF" modified="2022-10-12" version="1">MFNPQIDNNKNWKEYNNLKQQTYPILICYDSKTKLPIELKQVQYTVQVQPGLAVVQICQIYSTEQNKDQCELEYLFTIDQNSAVSKMIVELGDQKVYGVIKELEEAKKVYQKGIEDGKTMVISYEDQEISNIKKVKIGCLSPGKSLKIQFEYIQPLKVFLNQFWKLELSPIIDTSYLTVNELKNTHKDYAQQYSFVKQCVNIQAIQLNYKQNITVLINLGKPITYAKSPTHSILLNSDINMREKQIDLQNQQNQLSVTLDSNDPQNMTPNRKFELLFSCDDINKPYATLSHTNNDALQHIRYCATLTLIPKFNEFPIDDAYQSYLNGLNMPLQAKIQKSCYLFFIDRSGSMEGIRIQKAKQSLMLFLRSLPEDCFFNIISFGSSVKKMFNILQKYNQQTLNQAIKQVQEMSADFGGTNILQALVQGIYDENYSKNKYNPETLNVFLLTDGEDQPEKIIELVSKNQRPETRIYTLGIGNCCSEYLVQILAEVGNGKCQLVGDSEDINSKVIDLLEDSLTYYLKGFSLIHNIGKVSQIIPDPKSITQLKKNQELTLQILFSKQHKEENLEFKIDCFDPQMNKQISYEVKINLKSSKENEYFHKIAAHKLIKYYEKSIFQKAKQTDTVMINQKTLKDQDIIELSLYNQILCSKTAFICEVCQNEKDCLKLIKQKLIMSKRQEEIVNIPVSSSLYSRFYQFITSSINRSQPQQPEMCMAPIFKDFNDNFGIVEQKCQIDQQEFSKQANRFLPKQSIQCVLKPKILNEDYQILSYFELVDCMQANGCFLLEIDMEQKLKLYKIDNKYHLQDPCWQTVISLFYLELFFQDSKSSWQLIYNKAINYLQKKGVNYYQIKKECISDYGKLFQNEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UXE1</accession>
    <name>A0A8S1UXE1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ethanolaminephosphotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0510024</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000051">
        <property type="protein sequence ID" value="CAD8169391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UXE1"/>
    <dbReference type="OrthoDB" id="196717at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1760:FF:000036">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014472">
        <property type="entry name" value="CHOPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414:SF37">
        <property type="entry name" value="BB IN A BOXCAR, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414">
        <property type="entry name" value="ETHANOLAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015665">
        <property type="entry name" value="CHOPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="250"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="339"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8169391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="377" mass="43363" checksum="FF88BCB4C6BF34EE" modified="2022-10-12" version="1">MKYLNYDFIAPSHLHYLKSYRYKGTDQSLLYNHILSPLADFCLRFVPMNVAPNVLTLMGLACIILPHILYFFVMGDNFAGFIPNWLLWLTALLHMLYMNFDNLDGKQARRTKNSSPLGMILDHNFDSMIILLQGTSMTTAMQFGNTIFSVILYIIPSVPFYIIAHEEYYTHEMNLPVINAASEGTISVAVVFAATAYYGCDMWVQKLPWFFNYQINQFVMLMFITSLIIIMPAVFLKIKKFTSITSLLKQLRYFFLFNIVILYSIIFSQSNVIQNHVRAYMYTVGFTMSKAVGVVALNHVSNQKLPEYQNSIYIYVIIFLNTISGQILGQTIINEGFLIQFAATASFLIHIHFLYNVARQISEALNIKIFQINSINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UXY8</accession>
    <name>A0A8S1UXY8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0510143</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000051">
        <property type="protein sequence ID" value="CAD8169581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UXY8"/>
    <dbReference type="OrthoDB" id="289007at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8169581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="191" mass="23010" checksum="7406AE97383F49E4" modified="2022-10-12" version="1">MNSIPKQIKNILRVEIILISVLIMLNITLYFLNIAENKLKTCQLSFNAIERCYNLDQQLIYKQRMTKNQCDYCTVLLWLMPSNIVMLFMMTLIIINKGRYYIKNFMNKLHLVEIIYFGLQFVYNLFILFYIFKMPINQNYRTKLSPLIILFIESLINCFICILIRIDQIYTINYLRETNKKETQMQIQLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UYD9</accession>
    <name>A0A8S1UYD9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0470128</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000047">
        <property type="protein sequence ID" value="CAD8167456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UYD9"/>
    <dbReference type="OrthoDB" id="296632at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000566">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000977">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23189">
        <property type="entry name" value="RNA RECOGNITION MOTIF-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="13"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="196"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="105"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8167456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="363" mass="42037" checksum="DA36473687A4149B" modified="2022-10-12" version="1">MNNNSRDPPSKILLLVITQLSPTFPLCNDLLFEQFAKYGDIKKILIFERGKANKAFVEYYDIKDAIEARKDKIGKYLAEGEGKLTIHFSRLKNLDLEVVDKSRGTDYTQTSSTNSDVVKHSNTDDPNILRQQLDQFTRTFTQTPQRNMSPQRNEEINNLLNSDSEDEAKDVLKQKTSSSPEISEDVQNMIAQKPSKIIKIQSIDERVTAKMLYNIFNKFGNIEEILYEKQFKRVFIRYQTVEFAQIAKEYLNNITFFDQQWRITYIPLFQLQPTTIKDEYMIYYNPNGPKVIAPLSKTIILSGVSEPMEISEMIRLVVKVTEIKIISSDSLEISMLNISETLKIIAVFSDYEFKNQKLVISMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V017</accession>
    <name>A0A8S1V017_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0520227</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 9">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000052">
        <property type="protein sequence ID" value="CAD8170245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V017"/>
    <dbReference type="OrthoDB" id="10252009at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030837">
        <property type="term" value="P:negative regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000004">
        <property type="entry name" value="Protein phosphatase Slingshot homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043587">
        <property type="entry name" value="Phosphatase_SSH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45864">
        <property type="entry name" value="SLINGSHOT PROTEIN PHOSPHATASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="5">
        <location>
            <begin position="95"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="6">
        <location>
            <begin position="158"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8170245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="255" mass="29586" checksum="6E3733ED0ADC9963" modified="2022-10-12" version="1">MIPPPPPPPPPKQIEKEDAFRKKLLEKFGNFEGSTTDEIEKKLKKSEDIDETCFSEADGTGVLENQPLDTSNCTQCERCQKWIKNESYQHHLICHSSRILDWLYLGAAINSENEIELDLRTNVTYILNAAAECQNFYPQKYQYLKLHLMDTIGENILVHFNEAYEFLEKCRKEGKCALVHCQLGKSRSATIVIMYLMKHLGMNLREAFKFTKEKRYIVNPNIGFIRQLLQFDEQLYGKKSITAHEINPLCSVEFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V5E1</accession>
    <name>A0A8S1V5E1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0570150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V5E1"/>
    <dbReference type="OrthoDB" id="405996at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043812">
        <property type="term" value="F:phosphatidylinositol-4-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662">
        <property type="entry name" value="PHOSPHATIDYLINOSITIDE PHOSPHATASE SAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662:SF2">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-3-PHOSPHATASE SAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC" evidence="1">
        <location>
            <begin position="120"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8172550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="806" mass="93881" checksum="75A712CF9AD67C81" modified="2022-10-12" version="1">MIKPMKMYISSEKIHIVPDPQICGFDKSLTIDKQYFTIQVNQTMIESVIQEIGIFGIVGVYRFYGKLNLLYIAEANQVCKINGQIIYEIVSISNILQNQFSSMEQQSIKNIETYFSKCTYFSLQYDLTIPLSQQHQPEQSERSMRFWWNFHGYKTFLANSIPKCWCIKIIQGYVGLGLCEIKTQQKQKLTYILISRRETLRGGTRYNHRGLNNDGAAANTVETEQLIEYQEKLYCHLQIRGSVPVFWEQIGIRAISQITQTTEQTKMAITKHLMNLKKYFGSLLLIDLMAVDKPNEAIISQTYKEMINMINLPQIQHQQIDFKQYCKNYKFEKLNPQIIQNKDILIQQSYTKFQNGKLMNIQNGVVRTNCLDCLDRTNVFQTKIAFFITNLILNDLGINIPKEFRCETILDLSDQEKVHDLIKLIKILWGESGDNISRLYAGTNSSTTKIQKTGGSSWTGIIEHGLKGFHRFYNQNVTDEEKQNIYSFIVGENQQQNKQFLGMVEQMQQQSPIIHKNLLLMVIGYDAQNQTIKDFFHLIDSRQIPQFIIVLFQNIVKICQPCNYIKDQMQSSIRTQLGQYECAYASQNENQYSFIFTKQCIQNADFKDYILKQDDKELGIVQTFNYEDQRFQIINLNVNCQELLMYEKKIIEGIKNKNINFNISILGGICNSQILIEESIEDFCSKNKKDPISLLFQKDQLCQCFEISQLLKNYQEPNISFLPKNGSKSWANRILYGPLESQDAYNILAYEESISNGIRLLITIDFEILGLNSQKIINNEEEDDDIRYSQQFYQVTEENDDFEIIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V6Q3</accession>
    <name>A0A8S1V6Q3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">IFT80/172/WDR35 TPR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0570142</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the IFT172 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2186662at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030992">
        <property type="term" value="C:intraciliary transport particle B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.470:FF:000008">
        <property type="entry name" value="Intraflagellar transport protein 172 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.470:FF:000030">
        <property type="entry name" value="Intraflagellar transport protein osm-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:003383">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056157">
        <property type="entry name" value="TPR_IFT80_172_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15722">
        <property type="entry name" value="IFT140/172-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15722:SF2">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 172 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23387">
        <property type="entry name" value="TPR_IFT80_172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="IFT80/172/WDR35 TPR" evidence="9">
        <location>
            <begin position="624"/>
            <end position="751"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8172534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1733" mass="198407" checksum="807CDAF5A5E6033B" modified="2022-10-12" version="1">MQLKYSCNIMPALDGMQKVSGLAWSYNGQKLGVCTADRKILLFNDQLGKEDNFPTKPSDKTQQKSYVVRAIEFSPDSTKIAVAQSDNIVYVYKIGAKFKEKKTICNKFPASSSITTLIWPAQRENDIIFGLAEGKVKIGFLKNNKSQIAFTTESYVVSMATNRDGDVVVSGHLDGSVYSYNLDTQQSQKLVVHHSIPYALAYGQHVAAAGNDQKVSFYDNYGNLVQRFDYTQDERCKDFSVGACSPNGDIIVLGNFNRFYLYTYNQKRSQWEEIGVKHIENYYTITALCWKNDSSKLITGSLCGSVDAWDISMKKIRFKGKYDLNYIGPSQIVIQVLSTNQQAVIRSSQAEISKIDIKKERFVVAKTHETLIVGDLATGKTSEVSWRGSGNEKFDFTNENICMVFNAGELTLIEYGSDRILGTCRTEHMKNSLISAKVSYTGQNKTKVIAFLLDLQTLQIQDLTTKSIIATIAHDTKIDFLELNSHANRLIFRDKRRQLYLYSIKNAFKHTLLSFCNYAQWVPESEVIVAQNRNNLCVWYSIENPDKVTLYTIKGDVEEIERIPSTPTQAGSTSVIVSEGQSKVAYKLDQALIEFGFAIEGRDLEKAASILDKLELTSDTEANWKVLAQLSLEEQNLSVSEHCYAALGDVARASYLRGINRLISKYYEETGKKDGISYYKVQSKLAILDKQFNKAEELLLNHNEVNEAMGMYQDLHKWDDAIKIAEKRGHEQVKVLKENYYQWLIETGQESKAAELKENEGDFINAINLYLQGGLPAKAANIVFNCNMSFPQDLLERIAQSLAQSGMYEKGGEFYEQMEDDQQALNCYIKGNVFKKAVELAKRKDPKLVKQLEESWANYLVENKETESAINHYVEAGKFQKAIEAAVYSRSWTKAIQLLQNQSAEVSRPYYRQVAKHYEDVRQYDFAEKYYIKANSPIEAFEMYVKASKWDKALSIAREYLPEEIVQLYLNQGKKFEQAGKYKEAEKLYLTVQEYDTAIQMYQNLSQYESVIRIASKFTPQKLKDIHLGIAKKLERENVLKRAEQHYIEAGSWHLAMQMYKTHNQWEEAIRCCKMYGSEKETCQQAKLWAESLGPDAGLKMLQRLNLVDALIEYQSDRHEFEEAFKLANLHAKHKLQDVHFKYACHLEDERRYKEAEENFIKAGKASEAINMYEHLGDYTSALQVARQYEPQSVTQILISQAKFFIEKKDLQKAEQAYIQGKRPELAIKMYVDQGNYPLALKIAKSHAPHMVAELNNKYGNVANTYNMTGEDLYQSAQTWEEQRDYLKAIEIYLEVTPQNTQSEDVMTRAWERAIQIAANYDKDKYPRIVQIVCKRLIEIKKLETAAYLYEQVGQYQEAVTTYVQGREFEKAKQVAQMINNKELNTKLMDYITKEQRKYGASTGQANVMIETGDVAAAMEMLAQKNDWGQCLQLADKHGVEYLNKYLMRYVKITMQQGRFSETIQSLATYGMPIIQQNYPIYENLAIEIFVECDPKELKLLRQALYGFIQGLEAAHELKSETGKKFLKFGIVAHLLNLRNQYAQDGIVKLHAQTCISLLRYCDLVRIDQLYLDAGRVAKKTNQLGLAFVLMNRYLDIYEVIEDPDNNNGLGDGAEFQNSDLPSPYDVPMPEKNLISDKEKEEIRDWLLQLSVNQNQEPVLPTRQCDCGYQIYDASLKCFKCKQTWEPCIITGMPLLKNQTINCQSCGKGALKDAWNTYLQAYPTCPWCNKHAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VDQ7</accession>
    <name>A0A8S1VDQ7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">60S ribosomal protein L12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0620096</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8174987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VDQ7"/>
    <dbReference type="OrthoDB" id="285098at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1550.10:FF:000011">
        <property type="entry name" value="LSU ribosomal protein L11P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_uL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_uL11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_uL11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020785">
        <property type="entry name" value="Ribosomal_uL11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_uL11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00359">
        <property type="entry name" value="RIBOSOMAL_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL11 N-terminal" evidence="6">
        <location>
            <begin position="13"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL11 C-terminal" evidence="5">
        <location>
            <begin position="74"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8174987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="164" mass="17587" checksum="763B42FCEA1A0E2D" modified="2022-10-12" version="1">MPPKVDPNEVRLINIKVFGGEGGPASTLAPKLGPLGLNPKQVGDKIIAESGKWKGIRVMVNLRCQNRNADVTVIPTSSALLIKEIGGYERDRKKTKNVKHNGNLTLEQVIKVARAIEDKSLAKTFTGTVKQVLGTAQSLGATVDGQPVKTIIGRINSGELKVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VF65</accession>
    <name>A0A8S1VF65_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0620164</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8175083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VF65"/>
    <dbReference type="OrthoDB" id="305936at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="81"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8175083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="275" mass="32396" checksum="0F564849391CB869" modified="2022-10-12" version="1">MNQNELNHLIQKCYRIEKELGGNAEATKKVKKSREFDVIKDILIAKIFQISKLQDQRDEKMAKYGNDRDQIAMGVQIRDEIHEVERMIKQMGESLEKMSRNQKKYPAQEIELRSKILQKYRQNVDNLKLREQGERQEDTDNEKPLLLKDLQQQLYKNKPNREVVYQITKDEQEALDRFAQNDQIIDDKLVIIIDEVDNMKNKAQAIGTKIDQTKGQLKILDKQVDKTNLTLLNSNKQLKELLIKFRQPNKFCLDITLFLLFLGIIGVIVNMFYSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VHA6</accession>
    <name>A0A8S1VHA6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0650189</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000065">
        <property type="protein sequence ID" value="CAD8176650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VHA6"/>
    <dbReference type="OrthoDB" id="310762at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="80"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8176650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="145" mass="17459" checksum="78926C0F9E8992EA" modified="2022-10-12" version="1">MIFYCCRMQSQRSKWNTNNMKRTNLWSTQQTETIKKEDLSEEEEVQDNEDDQELELFKKRMQSAPPRYYIIRLSEDEDQSSSSHQLSQVEEQLEEQQEDDDTTRLSDESSIVQDYDQVPDDRPNIKPKSLEPFSFKKPYYRTDQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VHP6</accession>
    <name>A0A8S1VHP6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">cathepsin L</fullName>
            <ecNumber evidence="10">3.4.22.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPENT_87.1.T0640225</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="11">May be involved in extracellular digestion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Specificity close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity.</text>
            <dbReference type="EC" id="3.4.22.15"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.15" evidence="10"/>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8176211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VHP6"/>
    <dbReference type="OrthoDB" id="10253408at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000104">
        <property type="entry name" value="Cathepsin L 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035721843" description="cathepsin L" evidence="12">
        <location>
            <begin position="24"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="14">
        <location>
            <begin position="33"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="13">
        <location>
            <begin position="117"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8176211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="313" mass="36057" checksum="3BE636226930A876" modified="2022-10-12" version="1">MIKYLSILLITSVLLIYINNNTQDNITNLQQQFENWKIKHGIQFLDGENQYRFQIFKTNLQKIDQHNSDQSQTYRMGINQFMHLTQEQFESLYLINFKKENYVRDQPEIIQLRNSTLKASIDYRNYTVVKNQGQCNSGWAFSVTGTMEVYQKIYQKKNVSLSEQHIIDCDQLSRGCTDGSNINGYKFAIANGVATESEYPYIGYNQTCKRLNGTYHAVRYSSAVGEYNMKQALNSHPVSAGLDAQNWQFYSSGIFSNCGITLNHYAVVVGYEETGNWIVKNSWGLGWGENGFIRIKSGNTCGIVTYSYQDKYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VI27</accession>
    <name>A0A8S1VI27_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0630041</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VI27"/>
    <dbReference type="OrthoDB" id="284155at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="7"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8175409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="264" mass="31413" checksum="B80B0B7116E69BAD" modified="2022-10-12" version="1">MSFLESYDFLLTIRHLELGQIDIWKQKENGQLAYCKILQSDISKQELHERSNWEHENVIELLAAELHKQNNNSYISIYYEFYDSTLQDYLINQELPIAETEIWNLFYQILQGVMFIKSKNQTSNLCTTCIFYNNGQIKIMEGQSYNMQQQLGLVLFELCTRESQYLILEDNKTINMNNLNYMMNECKYSKLLIQKIFDLVNSKDTDQIQIIYNQLSPYKDNILQLKPFNFSITINTSLTDRANKAIRNYKEVLKRVNYMYQLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VQI3</accession>
    <name>A0A8S1VQI3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0690120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VQI3"/>
    <dbReference type="OrthoDB" id="294535at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="648"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="648"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="675"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="685"/>
            <end position="701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8178315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="727" mass="85155" checksum="694EBEDD0EBC71D3" modified="2022-10-12" version="1">MNNQQTISNNSKQEQQPEEESYDEEDDEDLQEYTQTTNLKQLFNNQDSFMQQINILNEQATVKIKQQKHKEALKLLQQSEQMLEFAASCGRVIDRNLIIIILYNQACAYQWYSYLYYYSQWILDKCSKYLDGVIYNMEIGIKEDEQDLQTLSNVQEKQAQLIKRQAFLVKAYLQYTAILSQLGKHKQALLNSQKAAQTMRELFKIANSFCQQWLQINGSQGTATTTSQSNVSVRNESSHNLDKKKKSQTYQLKDEIEFGRFVIDNAQDVLKDMIKQEDMSSIRLDQKQLLKETKRQLYNWRNKPDNNEKSNRKELKLVTQNEEYQSLLGIQNLAEWIKNFNIGSIMHMAPQIYEDFTQFGEMIFELAKRQLLEKVIYLSISYFTIATELRFVELEKAKKHGVKDDKIDTEEFKLSELYHLKSIEIACRHITYQSPYISHLITSYHKHYNMNLDVIREESLQSTASEKIIEEQQQSNVKGKMLQIQINKEITNTKFEKQDQSPKLTGNFIKSFLNSRSPPKQSQQQSVKNLISETVKIQTNLLEQMINKKRGSDSSPTNQRNSIKQQNQADFDFSIYLKKQSNPCNSSNLNTDTTQDIINSVMTIQLQPYKNNNIKNNLNNMIKQSSPTNNTQLKTLLSDACRTERQVMTEQQNSYTPISYRVNNRSPDSSYLNAQKQRQQQLHQNKTPQNRPRTNTEQQYLQPSFQLKLETIQQLLRNKAINNPKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VS99</accession>
    <name>A0A8S1VS99_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0750082</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000075">
        <property type="protein sequence ID" value="CAD8180868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VS99"/>
    <dbReference type="OrthoDB" id="282161at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8180868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="83" mass="9780" checksum="338CD7C78022F6DC" modified="2022-10-12" version="1">MNQTFFLDFQTPKISNNFNQIMYLPTFYKLGYEAWTAKTKRFMGIMPAVGCWAVWALYPNLYNTLYTDFIPPPKGVQRRVQDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VU16</accession>
    <name>A0A8S1VU16_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0720192</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000072">
        <property type="protein sequence ID" value="CAD8179733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VU16"/>
    <dbReference type="OrthoDB" id="314364at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035807647" evidence="2">
        <location>
            <begin position="23"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="46"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="273"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8179733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="384" mass="44619" checksum="C5666B95C30C3D55" modified="2022-10-12" version="1">MKILVLCLIALAIADKYDQFRAFDEDEFGRTLIDTLQMQMSTGEPIARFIEIMRNLETSIENEQKEDDKANNEYQNQCTEDIKVLQQESANLERRTVEIQSILDELEPLRSYKQGQADAKNAWKIETEKKLADLVKKRETEKAEFDKKVEEHDYATFVIETVRRMFSDKIQSFLQLNNESQWQKVRDYFINASEQAKKFEIKKSYSKIFNVLAEIASSAQSEDFQNSPTVNKIVNLCDLMLKQIEDSKALETKAEQKRLNMFMLEKGNFDKDLTSLNNALAQLNAAILGLDNRIQDQKRDLTDYNARLSAKNKQSEDRGGECREKAYNYQLTREKREQKRQLVSQIIGAFSANQRDFSEYVKLRGQAGDFRGKNFQILGNPTED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VV13</accession>
    <name>A0A8S1VV13_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0720143</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000072">
        <property type="protein sequence ID" value="CAD8179639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VV13"/>
    <dbReference type="OrthoDB" id="289272at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="245"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="10">
        <location>
            <begin position="505"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="57"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8179639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="646" mass="74282" checksum="B962139CC8E0E5ED" modified="2022-10-12" version="1">MRQRSGQILSPARVIQNFQMSTKNYTSKNASQSPLKFIQRNLSQIILKDLVKQDSSRKINNHGSITERVHSQQRSNSRDKVKPQTKQAKAPTEINLPFQQLTKGINLILNQKPTNKNKLILEKGSMTRRNSAEDLITNSIQIGGKSSSIDKLPKSEIQIYVHYSSEVIQNYKFCANITTDKVLNMLKNKSGNYQVVGFATLDENIGFDYYLTIPFLPLTHMIGKTIRMKPIFGISPPKQLNLSCFLFLHVIGRGGFSTVILSRSLIDGSFIALKLISKTFVIQNEKQDLVQNERDILIETTNKGSLFTSKIEFAFETKNWIIFGIEFCPGGEMFSYMKRVQKMTESQARFYITEVCLALGFLHHQQIIYRDLKPENVLIDITGHIQLADFGLARPNMQPEQNAYSFCGSPEYMAPEMFHNDGHNNLVDYYCLGALLYEFVTGLPPFYCEDKNIIYTRLLNEQVQFPKKLSPEIKDLIKLLMIKDPNKRLGSKNGVDDILAHPWFQDIDITKYIQKQIDPPYIPDLTKLSFKSPTTNDRILFEQLNREQKLINQFIPMFETSFFYQQKRYQCYEFDKNSSTQVTKDTIDSLVRKRTKNSQNSQQSLLSQQSQSQLSSVLGSNETLKNTKSVTNFKVYQNLLTEPSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VWD2</accession>
    <name>A0A8S1VWD2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0700010</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000070">
        <property type="protein sequence ID" value="CAD8178596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VWD2"/>
    <dbReference type="OrthoDB" id="289272at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="350"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="379"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8178596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="737" mass="86475" checksum="CB74BEA6626CE2DC" modified="2022-10-12" version="1">MPPKRSHHKTYSSEISPSNLLAYPTHREETKISKSPTLQFDFKTNQISIEQNSLLRQNGSSSNLQQLQQQKIIRKFQQQDLLISKGFITPQYLSSNPRLFQSNLKSKQQSNHIKTQPYSNKNIQYQVLGIETEEYKQPSFISQFQQKFQDTLHTQQVKKNSLSLKQKTEIPFLDLQQKQQIENLYSNQFHKSQGSFNIQNTQKISFHQNQTHSKSNGLAITPILQVHTPKEVLTEFHQLESLTARKKPILSLVLQYKLLKIKLQIELSTQNIQHLVDIVRQEINNNFKNCKQKIIGIQTCNLSIPTDYILSMTEKPLLILEGCQIKPLVIEPIFEIENEIQISRVSLKDFEFIKCIGMGGFSKVYLVKEIKTGKYFAMKLIEKGPIIQQNKQKIIQNERDIMCLIDDPFVVKMYFAFESRKYFVFVLEYCSGGELFFLLRKIKRMSEKDAFFYFSEICLGMKTLHSNNIIYRDIKPENILIDLDGHIRIADFGLSKPNMIETEDAYSFCGSSEYMAPEMLLKSGHTFQLDLYCLGALLYELVTGLPPFYSRNLEEIYQRILNQKLKFPPQIQLSSSIKHLLTNLLAKNPKDRIDSIDTLLNHPWMTQWGDQNLYKDIIIKKIRPPYTPDYFNLNFDEQEFGKGESEFLQFIKPLQKSMNENFPKEIILKEFYYNKNKNINEEEDQTNYKLEYKQTQKQNSKGKNAEEDSVPYHKVIKDYLLKLNQNQNQHEYKLNQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VY58</accession>
    <name>A0A8S1VY58_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinase domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0790078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000079">
        <property type="protein sequence ID" value="CAD8182694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VY58"/>
    <dbReference type="OrthoDB" id="304098at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8182694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="306" mass="35874" checksum="DC866C234C92A900" modified="2022-10-12" version="1">MNSNSPKLINQNSQSDNEIDSHENKHIKQDCVQIQAKSKKIQKRQQEQIRAKQIQIDQISQFKFDSSLQYQMNLREQTIYDFDFLKIDKDLLSNQNQIIIEDKKSKKKFLIKPIQSESDIIQKLFQLNSQFILTPENIFSYQVQTQKIDYLAKEIKIIHLKFQLLLFQYCSKSILHFTHELSNAKIKMIISDLLQGLISAKENQLVLNEITLDSILYCEVNRNFKIKDYTNATIGDNQSNVKELGICLAQLIQQKNSKKKLTKLQINLNEWKTNNKDDPFASITLQMIMNEKSLDEINNQLQQIDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VZM3</accession>
    <name>A0A8S1VZM3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0760048</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000076">
        <property type="protein sequence ID" value="CAD8181282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VZM3"/>
    <dbReference type="OrthoDB" id="309150at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="2">
        <location>
            <begin position="239"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="83"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8181282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="390" mass="45863" checksum="00F9B947A7DDA85C" modified="2022-10-12" version="1">MNNILCKHHPDNQIIFIIWNQNKLQLVCDECMGITHQEGILKDDKWKKLAIRKAFKEPEYFLQQFKLDDKNQSLMKSIAKCPESQLRDLIQRIEANLKDIQITLDKVINDLKGEVCNILNNKSNFRKQLEKVSEYSKFKELIESLNNTSQTITHDVVNQVETKLQEIFINLEQDTSNLNKEIQNFSVSTTINRIDMFQGIQQFKEQIKQFLNLIEPNNKFIVFESYFKDVLLNKISLDAGKTIKSTNLIFHENKQNLNHYLFWKCVQNKSNLLMIFKSQSGNIFGAYSPLQWVYEDKNIDDHSKTTFLFSYTHKSIHKLINQDKQYSIYFKKENGPCFSEDLYIKSDFQDGFSRLGTSYSSENSKNPSTHLFGQITPNIIECRIFQIIFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W0J3</accession>
    <name>A0A8S1W0J3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0780177</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W0J3"/>
    <dbReference type="OrthoDB" id="296368at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="323"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8182393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="633" mass="74632" checksum="6DD666083B2D6467" modified="2022-10-12" version="1">MQINKLNKTQVIEEKQNSRIYKSMQMGSNNKNSIQDYQVHLRADSTILIPFIDACMIEWNESAISEAANNNSGNYETNFLHFKHFLEDQCVFLKNNYDLAIKIIDYLDSKKLIVGYIKNSEAQLQTDDKARSTTNIQNTIKNIRKDIYRFRKELKDIMAICAEAETPLILNEIGNLKIEILERYNQLVNDIIPGILFDDISIEIVQNYYINQSNAQSDEVNHFNLIKSYVLSPFIPRFRAYVYQIYEIAQSEFNQTQNLLNKLQQIGKTASNQQNQSVQLYYDLPYLIDSYKVNLNQQCSLLPTTLQDDKDYWNYYQSNVQLKEKINNELINIKVKNEQEQQEKSCLQIEFNQLVEQQDYFHKLFLFQKQIPILIDQLKDINQEKDKMWKELPEHKVIQQERLTQMLQTCINISKSINDQFPHGRIPFKFQQVSMTADNLAKQIQDLMNFNCHQNQQNYVNNPSLPDRIKELMKWEYNGESIEQAQGENAEINSYLTQIFEFNSITNKPPQIEGELNQQLEQCNPDNINEMMELNENYNIWKNMQAAGMFLNEEYLYTTLKDSEGLPQYAKKLQHLKTFFSINQQMQYVGYITDVLSLIQLVQTKQLDQAKLFIQQSKNIPRIYKQNLLQALC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W3A2</accession>
    <name>A0A8S1W3A2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Dynein heavy chain linker domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0810011</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000081">
        <property type="protein sequence ID" value="CAD8183463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="299699at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46454">
        <property type="entry name" value="DYNEIN AXONEMAL HEAVY CHAIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46454:SF17">
        <property type="entry name" value="DYNEIN HEAVY CHAIN LINKER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein heavy chain linker" evidence="2">
        <location>
            <begin position="715"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2256"/>
            <end position="2332"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2402"/>
            <end position="2450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8183463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="3485" mass="419606" checksum="DC60F4E9BD44283C" modified="2022-10-12" version="1">MNQQYLRKKLKIKTTTDTPLIPKIMSTKNEQSQFIETNRKRYIKAFSPQRFQLPQIVQPQLSQITPLRQRGKSESVREYSIGFLGPNDFVRLLQTDQNLGQEFCYLNNYGNPYEWKVVNYDDRNMDYYMTISGRGIIIHHSNFEEFVSIKQFENDRKLFYRLQKIEFFRNYLKQKTFTRWKKLSIKNKAQSISKELTTSYLLLDQSLKRPIEEIHKYTQRIKGLVFFTPTQMSPVNQTKFIQKLQRSLNEFQKELAKNINLILKIVVDACQYSFSIFKEDQGYRQNLELIRNSQMEQKPLIIGDNSTQSMQFTMESNLRQFQQKLIKFTRMIDYIQLESMIILMYNSIVNFLKVIKNANKHLKVGTTQFSWIVVEIHLQEGTIVFEPSLKGMLDIFVQTIDKAIDIIEQGIQSTRKQQELQNYGVGSSSTDLRQDLREYGRINLKQYDYIEELGEELKFSYKQIEHYMLKIQILLDEFAKSEDQLLNAEVLKFQFQLISEMQTEMETIENIVDIGFLRLNIQLFKLQMQTFFKNKIQAINQNVDMELRGKCKILQETIQEWLEKLKLKPVTLKQYVIHQENYLLIKSNYEAIDQQIEELHVYNRIYIHQDQTIPIKDIWDKIVSQYQLFQYKYYDLQNQYGLVQDKFEKEFRKLTPFFHQKVLQFCEQFENNKDIDDINSISQLQGQLITLELEYEDLKQYSHSLQIEQYHNEQLHEIKNKYVQFKQLQIEIHKIKNLQQEWMREHFLDLNLSIIKTTYQNLLHQIEDCKIFGQSQLTQIKTQLINELKLYNVIEIILQIKDYDQNIVAQVCKKDRQQDKNLLQLILQICNNPSHPVFTTQYLQQSDINEQQDNLQQLLIIIQYENQTMNSLIKLEEFWKNHKLLIQKIKGTKDQYSIINLSQLNEQLDENILIINNILGQPNIDDLRNRIDLQLKNIVYLQEYINQLTLLQDTQYYLTNVLSTQINQKNQSKEMKMYQHQEKQWKQLIRLIQQQKSLEVWIKDENEKKFLKQIQQDNQNCKSIIQQLNEQFDKKKYLFPRFNFLTNSQFNKVVSEVKHPAAMQQYLSLFFQNIHQFDYDQNKEAVTSLYSKENDQIAVKYCPMKGEIEDWFKTVEEGMKQGLRQIIKNALKHADNYQQIVQYPIQIIYICMTITYTMMLDDILQNDDKDWNELVEFKQYEIQEQIKQLHSEKQSIKQQLRQIAIIMILNNQKHLIQEINQFKINSDKDYFYLKQIKYYYDDENIIVNFLNNKIQYQFEFFGDEFNYHSWKQSEQSILLMNQAFQINRLPHLIGHYSKYYIITQYSQILGRYYKQMQITSHDYDFQYLLQCIYGSIQSNSLFIISGYQYLQQDDRLILQSIFNQICNALWQQLQIQDQEGRQLKLQNKHFLTLISDVHYPILSFKNIHVYQPDIMILWQALIQIIYNQDTVEILMKLIQTLISVFGNDLFNLARFYSFNQVVKTLRTIKLNDLFDLLQYHMNNADKLLFNSIVNEYFQNREENRYEMEKLNSLLNENNLILVYGQPNNGKSKLIQELFQFKFEQENTNQNENNLLQSTKQNNILYLELDAYDDEDLFNILQHSQQYRYYHFIINFKQKQVYFKQENFVFFNSLIYSNRNVIFESNDLSTQDPASLVNFTLFYYTNSISNQQFLIQELKYINNLELQTLLARNLINQIEQYYSLLLNFPYSLQYVLRCSAKVLSIYYNEQQYSKYTQLDVENLSTYTTYTLFCLILDQENKSRVVEQIQMVIQERKLDQLFNQKSSYNNTILQYYFNQQELYAIQELQSHILFYGNISCNKSILAKLKSSSGLYFSTRTKSIELQEFIDKKLMTYRKDRQLYLSPPFGQIKHFIVEDINVSSQCRIFIKQLNECNYMFDKKNNFQLKYISNFYLLGTSQEQLIDKHNQFKHFVFINTSRSPLIQSIYTEIIIEMTKDKVKQYLELGYINDGIFKLQHKFKQCWDKQWCFHYLYDNNTIWYRILHPLLVLNDPQQFVNQFYQNIMRVIGGQMDIVDLDNLDKFVKELNFGDYPIEIFVQQTKQMYDEIKKENVQNLFLHESHFEKIICIMRGWQYDQHVVIQGRIGSGKNSFIRLAQLIMQYEQKYNDLGLNDLLQNWDQQNHITYVININDNVYEFISYPIQYCNNLCLLKNDQIQQLHLCILIENMKELEDYKRILYKCQIVRLFDWPKIFQQDIATQILNDRSIDTNLFCKVFEQSQGYIQQFIDQLYLFQKQYQQSKSENQRLIIIYQKVLNKIEEANTEFSTITAQYNKYNQEYQEITNQMDIINNEISKDKSELGDINQQINKLNENINKCEQKIIKQQQQLNQQFQQYSKQRLQHFHIIQEFPFLQPLSMELVHLVDLPQLELYQIEQINMKYPQFSQQVQTIYQIAINICNYQQTLIQLQNNIHNDKVALEEMKNKQELRTVMISQQEQQINIYQSKSRILTDELDKITKIFEATRKCLKYLNNYQVQWQQNIFNLQQQQIQLIDINFVQSICQSYNQQIKQKLIDNLNLQCKLDYENMQLLLNLKFQNQKNIILIIDPENLSQEYLQQFYPTAYVNTFHINNINEIKSNYQTQQCFIIKDVIINELLNSQQWRSFILQFQQQQETKIYFISYQIKYENKAQSLLRTIYFKKQDKEIESYALLNILQKENPDIFDKLIQQYDEENLSLINLGSLLDDDRPIMDSHYEQQLSLLISSQFNSIINLDIITQIQELVDQQEQQKQYNGQFNFEIYHPLMVRFLLIYKSLQQSYVFDRRYYITMQQLLDKLIQVMDQIKGDSSERYWIISNHFTLQIMHLIEHSFKLEHHLLMKFILFTQIDLRNRTISYDQYQYIIGQQVQKDLTKYPKSLNVSFIKQEQWDDIRYIMSLSPSFDDLHSQILRYPKEWQKWLDDTFSIPNEYDEKLQIFEKLILIKAFKPKQMRRFIYEYIKDRSTRHSTQIKTNKKYQSTEKQIIYVQSQLRKLVEDINIPIYKGQKELNQQYLFINLSHYPLEVQEQIIQSKYPHCIIENFEEDLLLDKTLLNCQKSFLQYDEQIRQNIKKYIPKVDGNTSEMKKFTYSLCYMHFFIIQRNLLLNLTSETYTVSDLNLILKFNLPYYINSNINSMFKVITDGIYNVPPEDQLTIESIIYKHCNQQVNTENYQYLHFQTLPIGSDVWLDEMINRIPDTNDTYSIGYGNYNFAIYNQQNQQIIQQFQLLTNNNIVQNKQITKVKLVEPLSLIKLEQKLTRKYSFQKNDSKPFSQYYRKTALNIINKNKQNIQYTDAIDQYINIQIQKYNILIEELKEYIKNNPHKYNMDCPQELYQYLWYIPKSKLLVHNLIEMINSNVYQLKLLRDSQNRRYFDLSYLYIPQALLEYFKLQFSRKNGLNPWNLNIYTEISKCIEINHVQQQQLNDGSYLLGGLECQNCQWSLDKHKLIECGNSPSTLIPYILVSTLEGEQPQELQIPIMDNDLILMTIDLQSDLDQDTINIRKARIVIKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W6H4</accession>
    <name>A0A8S1W6H4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Branched-chain-amino-acid aminotransferase</fullName>
            <ecNumber evidence="8">2.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0840108</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:18321"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24813"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.42" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8184991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W6H4"/>
    <dbReference type="OrthoDB" id="1732691at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004084">
        <property type="term" value="F:branched-chain-amino-acid transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009082">
        <property type="term" value="P:branched-chain amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01557">
        <property type="entry name" value="BCAT_beta_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.10:FF:000017">
        <property type="entry name" value="Branched-chain amino acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.10.10:FF:000027">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018300">
        <property type="entry name" value="Aminotrans_IV_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005786">
        <property type="entry name" value="B_amino_transII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033939">
        <property type="entry name" value="BCAT_family"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01123">
        <property type="entry name" value="ilvE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009897">
        <property type="entry name" value="PRK13357.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11825:SF44">
        <property type="entry name" value="BRANCHED-CHAIN-AMINO-ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11825">
        <property type="entry name" value="SUBGROUP IIII AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006468">
        <property type="entry name" value="BCAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00770">
        <property type="entry name" value="AA_TRANSFER_CLASS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5 8" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="4 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8184991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="398" mass="45194" checksum="ACD7CB65689F280B" modified="2022-10-12" version="1">MLRNISKVSTFQTHFKQIYSFSYYANLDLSCPHPSFQSRDLTITHTMHYITKPPCDPKIMTFGAFHTDHLLEIDWSEKMGWSRPQIVPFKSFSIHPFAACLHYAIECFEGAKAYRGANNTIRTFRLDCNMYRMKQSAKRLSLPDFDGAELQRCIEQLLKVDRDWIPDRPGFSCYIRPTLIATEEALGVRASSRARLFVVLCPVGPYFPSGLKPVRVFCNTSTIRSAPGGVGGYKVAGNYAPTVLPLKEVQKIGFHQNLWMLPDGLVQEMGVCNLFFFQKNEKGEKELVTPMLDGTILPGIMRDSILEITRGMGKFKVIEKKLYIQEVIESIESGRMIEMFGSGTAVSIQPIEAIGYNDKIYEVKYDTKLNAGELSHELFDLLTKIQTGGEDHKWIKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W7I7</accession>
    <name>A0A8S1W7I7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">60S ribosomal protein L37a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0640152</name>
        <name evidence="3" type="ORF">PPENT_87.1.T0770091</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8176067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000077">
        <property type="protein sequence ID" value="CAD8181826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W7I7"/>
    <dbReference type="OrthoDB" id="308782at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002674">
        <property type="entry name" value="Ribosomal_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050522">
        <property type="entry name" value="Ribosomal_protein_eL43"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00280">
        <property type="entry name" value="eL43_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L37A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01780">
        <property type="entry name" value="Ribosomal_L37ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="107"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8176067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8181826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="16399" checksum="1193FA8D02779B72" modified="2022-10-12" version="1">MTKKTKKVGITGKYGTRYGASLRKIIKKFEISQHQRYFNTFTGAHSLKRQAIGIWRCTQTGLQIAGGAWEVNTPAGLSAKQGMLRIKKLKEDAEVEVKDEKKDQKQKQQQQPKEQKEQQQTQQQGQKGKQQQGGDKKRGQAKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WAM5</accession>
    <name>A0A8S1WAM5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0880237</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8187278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WAM5"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF263">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8187278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="212" mass="24377" checksum="3192C6B13E70AA62" modified="2022-10-12" version="1">MGMTECKECHQASLEDQLPQPFQLSIRGRSHLISITSYQGEVKTLSVEPNLEIAYPLTQEELVEYEEQLKAPIKQRLQELEPLQIRPLKSYEYSERAYKLKKTQSIYLGQWNNGIPQGFGIIINPIENSYYEGSILGGKPHGFGRKIYSQGNYYQGEFEYGKISNQGLVVIGKEKDDTKELNQNEYLQSINQNMKIDSIQTERSQVMSSIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WFS9</accession>
    <name>A0A8S1WFS9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Arylamine N-acetyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0930035</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000093">
        <property type="protein sequence ID" value="CAD8189104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WFS9"/>
    <dbReference type="OrthoDB" id="3349226at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2140.20:FF:000008">
        <property type="entry name" value="Arylamine N-acetyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001447">
        <property type="entry name" value="Arylamine_N-AcTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11786:SF0">
        <property type="entry name" value="ARYLAMINE N-ACETYLTRANSFERASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11786">
        <property type="entry name" value="N-HYDROXYARYLAMINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00797">
        <property type="entry name" value="Acetyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8189104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="286" mass="34457" checksum="E94BC617E580DF13" modified="2022-10-12" version="1">MQQLISQEQYQTYLKGIGLPKDIKLSVSVETLYLIANRQLIYNFYQNTELHLKELKPISLEFVDILQRLCVQKRGGLCYEHELLLYYVLKYLGFHVELIRCQVREPNSPYNPDLPSTHAFLNVRIGDETYLVDPGFGTRSYRFPIKVNFQDLTQTHELFAQEHYRIQENETHYQFQHHIDENWVTYYDFEKPLKYASVEDIHNDYLNLFTCKQFLGVRDSRFVICRNTEYGRDQYLWFRLEKEFTAFKKVLKFNEVSKIEFKSYDELKEDVKKEVGFELPDYELLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WHH8</accession>
    <name>A0A8S1WHH8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tc1-like transposase DDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0910137</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000091">
        <property type="protein sequence ID" value="CAD8188431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WHH8"/>
    <dbReference type="OrthoDB" id="10006939at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564:SF1">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="1">
        <location>
            <begin position="2"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8188431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="114" mass="13475" checksum="FFF9E2FF45C79C54" modified="2022-10-12" version="1">MQQVRFIVTTLYNQLGLNAVLVLDNSPIHKSKLVQKLLTAKPYLFLPPYSPQLNPIEKIWNIFKQFIRKNQQVPTQNLHRMIHGHFNSITNEQLENYLWSLLKELKKSLDSVDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WI63</accession>
    <name>A0A8S1WI63_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase M20 dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0910073</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000091">
        <property type="protein sequence ID" value="CAD8188307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WI63"/>
    <dbReference type="OrthoDB" id="3064516at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051458">
        <property type="entry name" value="Cyt/Met_Dipeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270">
        <property type="entry name" value="BETA-ALA-HIS DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270:SF4">
        <property type="entry name" value="CARNOSINE DIPEPTIDASE 2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8188307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="430" mass="49447" checksum="3E62209D60DDE3BC" modified="2022-10-12" version="1">MRHFIKQFSEQSIVPSLSNFIRIPNLSPQFDNQWQQNKLLEEACNHIIKWIENETIEIQKEIQILQIPNSPNCISIKIFGNQKQNKTILCYGHYDKQPHFVGWKYGPTTPIIENNRLYGRGSADDGCVPYAIISAIKALKEFKQNYHDCYLIVEGEEESGSHQLIQYIKMLNISKIDLMIAIDSGIVDYDRLWITNSMRGAITFDIKVFQTINPFQYLRILLNKLEDSLTGEVNSQFQTIIPKSSLEECQTTAQLVPLEFPMTCQKMESDHVQQYINRAWKPSVSYIGGLEDLDQDNDELIIRISMRLPPNKDAQEASQQLVQLLTTQWSQVEIIQAKTGLAVKSFSKELKNILNEASIEYFGNEMRMFHEGASIPFLNSLYQLYPESEFLILGVLAPDSNAHGPNENLNIEYFNKLIGCLTYIFTSQNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WIY3</accession>
    <name>A0A8S1WIY3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0920007</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000092">
        <property type="protein sequence ID" value="CAD8188627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WIY3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8188627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="114" mass="13815" checksum="F6750D85F8232258" modified="2022-10-12" version="1">MIIIQYQNHFLILFKLKNIANVIKKFIQELDEEILQCMKQYFSQNWRQKEQQKTSYKISKNKIMIHFLSCLNIIQILVLVIQFIQNLKKTLMIINIDEIVMISQDKMKRDILLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WJN2</accession>
    <name>A0A8S1WJN2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0920180</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000092">
        <property type="protein sequence ID" value="CAD8188940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WJN2"/>
    <dbReference type="OrthoDB" id="282544at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8188940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="222" mass="25952" checksum="C591108B530B6039" modified="2022-10-12" version="1">MSVRKIHRDPGPQTEGYKGVFKGVSENLNVGVGKYAPPIALEQREHHYNIGWKPWQQSNETVYEVDYKPNQEIVKRENCANMHPPTIHIDHINVPYKKDTHFNSEYKSYKDYQNPPRAKPIRDKLTLMATQIVMGDDKPNYKTAQLIAQEPKPPQRQLVYHPKPEKYDIITNHEANQERIKKASAFDYWNPITEKKHQSFNTSEIPQYRLGSRDPITGRFIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WMN2</accession>
    <name>A0A8S1WMN2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0940042</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WMN2"/>
    <dbReference type="OrthoDB" id="18487at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053215">
        <property type="entry name" value="TKL_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45756">
        <property type="entry name" value="PALMITOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45756:SF1">
        <property type="entry name" value="PROTEIN KINASE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035717738" description="EGF-like domain-containing protein" evidence="2">
        <location>
            <begin position="16"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="910"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1026"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1060"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1087"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="181"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="253"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="361"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="403"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="449"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="567"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8189469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1137" mass="129352" checksum="2C99DFC79ACDD190" modified="2022-10-12" version="1">MFIIGLLILIQITNTENSYCSCDNGLENGIYCFKTIQNCLDCSNYIVNEQDICTSVTTCDQTTQQQKDCKTCIDNYLLSNGQCWQKILYCSLQIDATCQKCQDKYLLKNQDDKTQICINSNEILNCKYYLQNGKCNKCDNNYHVSINSTICYQDIKYCDIHLDDKCTSCITGYSKHTNSTFCFPDILNCQITQTFYIDETQKTYCNKCNDGYQSTENKQCSLDDKNCLLFNQDLTICLKCLNDYILIGKKCEFCPIKFCSTCGDSQIFKKICSQCQSGYVKGSNALSCIGQCESQTSTMTNCVSCSNSTCSKCSDGYFINPQGKCTACSAKYASCEQCIINKCTSCSNNYYYDNSKQYCFLCSTFNTGCQTCLNTMQIGTTNNIICQKCESGYVLTSTQQCTKCGQLFENCDTCINLGNNFQCNTCLPGYLKLTDTSESTINPTYYCLDCSGSIINLLNCYKCEVQSDTSITNQYICKLCKDGYYLEEQSCLKCPNTCTQCTSLTTCTKCQTSLILNNGQCTDDTTSFQLTSSANSNDALAYGNILISKSSLGPFPFIGTLNSKGFFCQSDKQCNMYGKCLLISCKCIQNISGSRCQFTTTSDFIKLQTELLTYLNTQTIYDPIFVDIIQLISDTTYAVTESNIKSFQQALEKLIALKFDLKYFTTIGAISKNLFNQENIASQKLNNLDNSLFNSILKQSESIMISEIQNTQGPFHKQIIGKFDLFSDDSYSTFKYNFVKSYGTCDNDKKLLTPQIYFTEENLAEIKILQSDAQTPLIFNQMHLYYQNHRSSGQTINSTISRIQLIKSGKILPVSGMNMVIVVPKNDQYYADLNENNVCVQWNEDKQIWENTKATLLKCKYYTVCQVNNLGDFGTYVQLVRNVTNNSSGLSSIFEIPQEFKFVKTVQNSAFAISMTLIGLFLTFLTLLCILYKRPIKKKKQKKYSDIYETVQTGNVLVQNPLNQVKTRKKSKNFWSVYPINAIFTASSLEFSLQKLSLYIIQIQMYVTFTSVYLSSLPFKFGMLTAYYLLVIIQTWICNYLYGGLALAFRKKRKGTVFGFYWILWVILFLGNMIIGIWQMYGLEFKFDMYLLVGVLIEFILDSIFCDLFLTYLYQSLFINEVVGIFKIIKFKGYYEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WQ71</accession>
    <name>A0A8S1WQ71_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BAH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1000042</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000100">
        <property type="protein sequence ID" value="CAD8191853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WQ71"/>
    <dbReference type="OrthoDB" id="306997at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04370">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46364">
        <property type="entry name" value="OS08G0421900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01426">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00439">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BAH" evidence="5">
        <location>
            <begin position="76"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="332"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="384"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="432"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="603"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="806"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="384"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="432"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="444"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="609"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="621"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="807"/>
            <end position="830"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8191853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="882" mass="102567" checksum="D79A0C0A80B94477" modified="2022-10-12" version="1">MIKSQYCSQVADYSRQIKRQSPESSATIKKQIYKRLQKRQTIRELYKQKSWKTLVKVGSQIKGLQEEYDTIKVQGQIISVGDSVLINSGDQHDEDYVGTIKQIISIKEPTTAKLICLCKIQWYMRKSEIIKSQPKCSEWISEQELFITNQQEYILAQSIITSCKILSCSEYQELDEIDSTIYFNRLEWDLQKKQFSNMDAIQQYCFCFQPVNPDRQYIQCDSCKNWYHFECVGIQNGTYDQNEFHCRKFFINQTIIAIQIGKQMQTSFKFRAPCNLQYFQQEIIVENEIPELDTFEEFCSNIPDQDNQDINFSKQSQTHNSQLIRIDCQQTHESKSSELQQSIDSREQKSKSKTKSIQSQENEHITYMQFVEFNPHSIISESFNQRTDTQQSQKDTMAEPPAPQNKNTTKFCFPSKGSGLNNLKKQLCFNQDSQNNNKNQSNSEQTVLKNQSLSENKNNIEDDLRKQITQLCKEKTQMQLQYQQQMKQLLEQNQLLEQQLILQKQQTQSLNSDKQILQQKINILSKRQSGDKNQIVENCNFSESVPSKLTNNEKIQYLIEKLNQKCQENARLQYEILLLQNNIELLETSKILQLPNNNIFSLDPGKITNQNHSPQPQSQNRAQSLSKDSRTSLLTQQKQPLNITINYPAESKNIVNLSAMISPLSKCAQIIQQKQLNQKIQKNNPQFQEDIQSQKIHTKNSTSVGELMSMKSDAINYSSIINRILRKESYSISSPDGRATQKFKQALNEKLNNLQQDSSKSYGSLLFSLGEHKLKEANNCFRQISPKREKKKSKQLFGFINQQNSRKQSDLLKSEESHRSDLGRRSENKNGIDPNNEIIQFMKEMQNKKSDISKNIKQQNTGTLLTRPLIADIKQAKTNRQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WQZ3</accession>
    <name>A0A8S1WQZ3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0980127</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000098">
        <property type="protein sequence ID" value="CAD8191190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WQZ3"/>
    <dbReference type="OrthoDB" id="298737at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035927953" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8191190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="214" mass="24828" checksum="16B10D4FA4EBFAB5" modified="2022-10-12" version="1">MRLVFWIIVFLSLVQAIPKKQISIHNKYNTPINAQDVKLSEVDLALYQNDMQQIGQKNHLKSEITNWMQAKTSLLEQLSAFYNCFEIFLSKQQQIGILLVCVICFAFYLMVKSEDRNMKQKKQNTKKKQKNCQQDEIVQILTESLIAKEQCPLPQVSTINGSLHLEERVNKSADQMKNNEQFTLHKRSNSQPIIQPNLPDYSAEEINSLLKDIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WV89</accession>
    <name>A0A8S1WV89_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1050003</name>
        <name evidence="2" type="ORF">PPENT_87.1.T1050004</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000105">
        <property type="protein sequence ID" value="CAD8193708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000105">
        <property type="protein sequence ID" value="CAD8193710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WV89"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8193708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8193710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="134" mass="16002" checksum="453F3B7641E06C32" modified="2022-10-12" version="1">MQTILTKLFQELKLFIYNKNQGDVYSVFVCNIKIIKLGQLKEIISSLNQLVDTINPQAIEKSEQNLHQYMQVQFKIMGLIQIKNFVLFQIRNIIKLRIQKKRNFRNLKNLANYINKFQNDILIQINGQIQNYRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X7S8</accession>
    <name>A0A8S1X7S8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1140097</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000114">
        <property type="protein sequence ID" value="CAD8197069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="302675at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="924"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1116"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1237"/>
            <end position="1260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1492"/>
            <end position="1513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="848"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="857"/>
            <end position="867"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8197069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1553" mass="183804" checksum="69932B5935F19D0D" modified="2022-10-12" version="1">MITKVKLKILLKSFFTFYQLLIVIPLIEHLDTHLFKNEDLLIGIVTLIPLTLCYIFFFLIHRNYLLPQKNIYLRRYSYYNILLIVFDISIMIAIQIVDELISSILIIFMSLLLIIDSFIIQPYSYQVNLKYISATSFTFCVSTIKILSFKISQEETFYCFIILIPLITIIFTQVFQRLVNNQIFEHQLGLAKLLAIIDLINDKKTNHFQMISIINHIKKQYKIMIDSDNIYVKIQQTVIQLFDIVIAQVKVQDSTILEQIQLYKILYITNTCKRVSLGYIELKIFIAASQRQSVYFSIISNLLQEKMSKRKKQVNDQDKRLSLIDIRKAELMYEDSINFFVNILDMKIKFWIDLEKGYQRIEEFALSAYNLSEKIWYIRDFFFQQFSIEPMKLQSRLTHFNILELKLLAILYSAILNDYHATLNIEQKIDEILQNEKNSQNEYINNITLINEDLVMMAVSFVKQKNQILNKNRGQIAKFFGYENEKDFMNIISIEQLMPQYIQQYHETLLKKYLKKGYSTFFDQSKEVYIKMKTNFIQAVNMNLLHMYEQDSDYIITGALQKVKQMYDFIIFDRKGKILGISEQIFKILGSGFSIEQLLNKGYIYFWIPNVFEIISEEKGQLLESSDMQSRSMLKQQLLFSINNFNQLCQDHELSRTNSTVKNKESQKFYTELNETEQMISVGNVNSIHFLKDQNVSLINEFLIKYNQSKYVDQDNLYSMKYSISFNVIGGDILPQFVIQINEIEKVNPKKQGQTFSMSFYSTIKKSTINDKITESCFVLEHEMNDNNQNLYINPPVINKMSRIQDSKIEDSSNKYPIYNQSNEQFKLISPRGNKEILIGIDDYEDEDDDQKFQDAPKSSSQQHNNQPDVVQAIQQYKELDQEFKENAKQSQSSGTSDRTQMSVFNILKNLQYSQRYQTTLIKVFINTLFLFTILLFLIVLQLVISRSNTNNLQYQIPLVGLPDEFSRIYNTFTILGQMDIEIKILNMTHGDYYDHRIEQDSSILREKLLVLIVEIEKEFSKLEQQGKLNQAQIKIVSQYSFDQFNISILQFNQMVNEYTEILDQLFDIDNKEDVVKEQIRLMFFKANLNNLLDFVSNLINNIVNEFFTNIENYELLYLIFLFIELLIIIVAILLQFALWIDPYVYKQKILLMIQRVQEKEIDLLLLKYKSYKEILLNDINRWKQVNYFKEFFTCRISQLKKIQNLKQQHEINKKQQKLQQRAKINTRIQETQYSVIHIYFMLLFLWLVMTSFVLSSYLYQYINITNSYPELNLCMQFVRFKQEFDATMIISQLMKNAPLLSQYQSQSGIYSRNPEYLKKDGFFKTNNQQLLSQFKSSYIAGKNHYSTIYQNIVASTKISDTNKNLLIGLYQNDLCIHIAQKLPFCSYENGKFTYFPNYPIPEEINNNRERLKQGFNGIYQEVSSLFASKFVKELNGDLETNSDEIKNFLHTPEYSQILLPYFYDLNQAIELFYDTIIATSVDILELDYQNYLIYTIIYGFSVSTILFYVIIYKTRRLQRQIRAIRQALILLPHESLLDVQVYSAIKRFEQGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X937</accession>
    <name>A0A8S1X937_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1150147</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000115">
        <property type="protein sequence ID" value="CAD8197520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X937"/>
    <dbReference type="OrthoDB" id="436852at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="551"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="220"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="554"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8197520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="634" mass="74085" checksum="483ACA8A4BFE2C54" modified="2022-10-12" version="1">MKINQPKFKTKKPLGLTLDYLESQQHQKEILPEAQEINQFDDIFNQYQQIQQTKELDQLNQSKSSNIEQHQNDQLNSSKPINLSISIYIIVIFYSDVDNQNQKVQKIIFDEYYTQNNGNTDKSDNIQIQQDKLSNINTKLIQEPPAPKNKDCVKQSFSQKGSSLSGLKKQIQIAIPDVEHNITSSYEQTMIKQQLKQNQMNDLELKSQIFQLQNDKDRIKNQYQLEIIELNALIQELQNKLEIEQAEQIQLKQQNQKLTNLISQIDNQNIEKQDKEQLRCSHHNNLNDKEKIQYLIEKLNVKSTQNAKLHYEIIKLQNNVEMLETQSILHLNNGNQINNQDKPSINQVPQYLGIEVQQPTQVQNKKETIKASVIMQQKQSFLNLSANHSQLDQNKNMNFSNMITPQANKNEVSNSIHKNLTLHQTKNGYFKEDNLQQIRKIQSTKNSTSVGELNIIRQDPTNYSNILQNMMKKGAPNSPNKYIHATQKFKETLDSKLNNISDKQKSYASLLFSGGDMKKSRHKDAYCRQYSPHINEDIQFKTRQLFSLKPYSNSRKESETIKSEESHKLSTDKAKYQNKYEYSNYQKKKDILQMVKDINNQKIGISAKINYKKKCDATYEQQSNLRQYNKNNLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XD04</accession>
    <name>A0A8S1XD04_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1190123</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000119">
        <property type="protein sequence ID" value="CAD8198682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XD04"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8198682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="82" mass="9758" checksum="D3616B09F6344C6A" modified="2022-10-12" version="1">MLLITIHQKQIEYLEDKVGINSNDLDSPYQILLGIDFVASLQKFIESLDTNILRIMEYLIQSNCFMKRQQFLYEMVVDRQEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XDR5</accession>
    <name>A0A8S1XDR5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1200141</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000120">
        <property type="protein sequence ID" value="CAD8199059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XDR5"/>
    <dbReference type="OrthoDB" id="2135133at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34726">
        <property type="entry name" value="GBP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34726:SF3">
        <property type="entry name" value="GUANYLATE-BINDING PROTEIN N-TERMINAL DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="415"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8199059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="526" mass="60892" checksum="DEFB741690CB5F20" modified="2022-10-12" version="1">MSSPQEGDIKLDQDAFRSGLQILGNLLKENLDLLETFAELFRKKQSINEFFDLYEALHYELKIRCEETLTIISQLNNPTIIEKSNEIPNIITNFQKPSIQEYNIIDTQIYDLVMDLNMSSSWKLLVNNNDLKGARVAVLGLYDKGKTHILNQLTNQNLPTGSKITTKGISLKQCIIDKNNSFVLIDTAGSYAPENLKNLKEKQQIENAILEISFELTDYFICVVNDFTSIEVRYLDQLARKLSTSLKQFKEIIVIHNLKDITNPNLIQHVWSVQIEKILEGNIQKSTVEAINPFTNQLEEKSVMWFNTETSRHICIVNDNSDLGKSINPWTYSLLKYWLKLVVVPHQSKILKYNDIVEVCQLKMSNLLKKQIKLTIQDTHLPNEKRIIKQQAISPKFNSTLITPKFTSTPIDIDNEEPEEYEPQIDIIQGTQYIILIDLPGVQLEEVEIYRQNVTTIIRGHKKISLYTNKSTYLKQERKYGPFDLRFKIPDEYDPKWKYYGMNNGVLGIIYTLDSEDSILPKKNQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XFQ1</accession>
    <name>A0A8S1XFQ1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1220151</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000122">
        <property type="protein sequence ID" value="CAD8199793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XFQ1"/>
    <dbReference type="OrthoDB" id="311376at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013906">
        <property type="entry name" value="eIF3j"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08597">
        <property type="entry name" value="eIF3_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8199793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="170" mass="19762" checksum="06956DB5A08F185A" modified="2022-10-12" version="1">MSDVEDWEQYADEEQEEKQEQKQEDKSKQPQTQQQKSEVQSKESDMNNVFDLFGGDKPVKKNIGQVDLKKIGENFAAALQNVGPNYTTEFLKTLLTKLEGKLLLNHYEELFKTVEEIYIKKRKQFEAEEAIKKKSQQQGPPKGISFGKTKNIIEDEDEDFDNVDDDDDFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XGD0</accession>
    <name>A0A8S1XGD0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1230013</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000123">
        <property type="protein sequence ID" value="CAD8199918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XGD0"/>
    <dbReference type="OrthoDB" id="10390353at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8199918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="186" mass="21884" checksum="88796404BA576CD6" modified="2022-10-12" version="1">MSSNISNASVKTNEITYMIAEYNFEEEMLEIAALQQTFSDQFDDLVGINIQALEEKQKKDQKLNKMKFRIRKSTKQIRKGNKINEIQVKKQSIVERSPLNTDYFNNHYFEIKKYQGEWQKQEQEAFEEQLAIPLYQNGFQTQKLFYKDQQDIMDINRVDLSINRSINNLNKNSMNLSSSSSNLSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XS34</accession>
    <name>A0A8S1XS34_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1350098</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000135">
        <property type="protein sequence ID" value="CAD8203920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XS34"/>
    <dbReference type="OrthoDB" id="312831at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001303">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="30"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8203920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="391" mass="45590" checksum="7C2F9725BE22E953" modified="2022-10-12" version="1">MQSTLPRVRFQSEYPVKKVVSPLIELPIEYQLVEKAGQGSFGEIYKIYNTLTRTLHACKVEFKDTRQQNQQSLLVKEYNTFQQLKGIPQVPQPYQLIQQQDYKLIIMPYYGSGNLEELRKKQIHEKFSESCIIRLAYCLICVIEAVHERKYVHRDIKPENFLVGSFGDHHTVYLIDFGLAKPFVDAQGIHIQQADNKGMVGTARYTSINSHLGAEQSRRDDLEAMCYVLLYLYNGQLPWQNFNCQTRQEKFMKILDAKQKFASGQLEINIPPILKKIYDHSKGLRFDEQPNYGKMKEICKEGFNKTRPYCFDWCVVDCKIKPFEDINDGQSELASEFSTIRKQLKGQTTDQISTNLKKILPCEQIKEEDEESISELPNVQKITKFYQNKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XT45</accession>
    <name>A0A8S1XT45_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1350102</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000135">
        <property type="protein sequence ID" value="CAD8203928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XT45"/>
    <dbReference type="OrthoDB" id="301294at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00582">
        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CID" evidence="1">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8203928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="254" mass="30210" checksum="3DF2E7238E3878B6" modified="2022-10-12" version="1">MDLNQLECLLQQLTEAKDQIIKTANYCSAYPGSAQQIASMIENEFTQTRKDKKRIYLFLIHQIILDESQLRRQDNPFLKAFGGKLKKLLGDFCIVDDIKDLESAYTMIKRWERDLIFHPQFIQKLRSILQPKYDQLKQQQETFNALNQTDASANKQIEQSHKIYKHLQQLADIDSKTLEFNCNYDKFDEKTTSNQDLQNLIQEGEQCREQLIQSIIHLQQYYIEMAVQGENLQSEFYQGMQKKIDYYKEKKKEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y1H2</accession>
    <name>A0A8S1Y1H2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T1460084</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000146">
        <property type="protein sequence ID" value="CAD8207165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y1H2"/>
    <dbReference type="OrthoDB" id="28255at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="4"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="428"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="557"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="625"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="7">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8207165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="660" mass="78338" checksum="BD40BB66AEEAD7B3" modified="2022-10-12" version="1">MQDFKDMKVIACGQYSQVFSAKLRKENKLYAIKKYIKKTLSRQRISPNIAKEIEIMNLLGDNQYTTRLINHFEDKESIYLITEYLNNGNMQQKLLKDKKLSEQETLFYAKQMIDILEYLHKNNVVHRNFKLDNILIRKNEKKQITIKLCDFGFSTYIQHGEGIFNGNDHFGPEQYNLQPQTEALDIWSFGVILYQMLTGKFPFEPKPEQLKGKKKQDVLQHNIVNGIFSQKNLSEGVQDLLIQIFQINPSKRITISQIKEHWWIQGIQRPNDKINIISILEDEPETQEEQQQPIQQCIDVEEQITQIRLSVKKKIQHNFKIQDNVINNNEISEIQILQKINKIEVCESEEINLSQMLNTIKQQLTQINNENMDLMSELQLRSSGTVHLSDERESVVSYLNYDSKSRQDFCKIQMQQIPRIEGEQFCLKQTLEQQMEQLQQIKENKESIDKYKEDIINNNNYINELMQTRLLLKKQIQDLEEKRSFMKLEEGIIGIQQEIEKKLTQLSTDFNDRITILEIFNNTFTESKLEENRQQYKQHKSIEKKYDLSLLQNEEFPEKKQKKIDQLKQQIQETREKQNQIINQISVNSTLFNQKDEESIKEKLNLQMAKKDEMNIQLQYLQQQTDILLAEIKEQEKGLKELDQKKLEIEKSNQKSKIVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y2F2</accession>
    <name>A0A8S1Y2F2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">V-type proton ATPase subunit H</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1490135</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase H subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000149">
        <property type="protein sequence ID" value="CAD8208099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y2F2"/>
    <dbReference type="OrthoDB" id="10263554at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004908">
        <property type="entry name" value="ATPase_V1-cplx_hsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011987">
        <property type="entry name" value="ATPase_V1-cplx_hsu_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10698">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10698:SF0">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11698">
        <property type="entry name" value="V-ATPase_H_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03224">
        <property type="entry name" value="V-ATPase_H_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032184">
        <property type="entry name" value="ATPase_V1_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="1" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase V1 complex subunit H C-terminal" evidence="2">
        <location>
            <begin position="323"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR032184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8208099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="438" mass="50951" checksum="89D54421E8C47FF6" modified="2022-10-12" version="1">MFKQRRYFCQSITFYQREQYLNKVMKDCEDCFKQLDQHGKSIKRDTKDMFIKFTQGKLQEQKKLLETDSVGVFSMVLDIIENLIHDKPLMSYILTTIEAIISDNQRLFKQFMRALTPQVLPKLKQFLFLDGYDPMAYEAAAKIATMIIAEEGGNDAKEWVILFLGGIGNKLKIADFMIMPICVHFLKHEELAIQFIKSGGIRIITNLLSKYSTDLQIAYYTILALWLLSFTTESISLFNDPTIGLIRLIIESVQKISREKILRVSFACFRNLVDVSPQCIELMVDNGLIKVVDLLLKGNLKDQDLIDDIKYVGEILEKNMKILTSFEKYVKELNAQNLTWSPVHTEKFWKENVKKFEENDFLLIRKLAEILKSNNNQNVAVACYDLGEFCRFHPFGKVVLEQLNAKQEIMTQARNNDQQIREHALLSLQKIMLHNWQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y2Q7</accession>
    <name>A0A8S1Y2Q7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1450131</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000145">
        <property type="protein sequence ID" value="CAD8206938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y2Q7"/>
    <dbReference type="OrthoDB" id="297509at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8206938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="183" mass="21808" checksum="E9675447F84B9A16" modified="2022-10-12" version="1">MNQQEQTNIISADPFLQTFFLINNLYNQQIFQYMNSQVQCVPKVECREENTKILEDSNNDKKIQQHYYITSNQNQPLVRTSKGVINKGYTHRNDVDTSQRVNGHWSQQEHQMYLQFVNDHQDILKSKYDKKSKKIFKLMSQSIFTRTATQCRSHHQKFNPLQKSKRKEKNNLRPIPTVITQEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y4A0</accession>
    <name>A0A8S1Y4A0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1490159</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000149">
        <property type="protein sequence ID" value="CAD8208147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y4A0"/>
    <dbReference type="OrthoDB" id="318921at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8208147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="743" mass="89611" checksum="86ADAD0F61CE9EB5" modified="2022-10-12" version="1">MQYLFIIFGFHILQIYSKSKTCEIQQIQNKITCHEYILNCFLLEGNEEDYYQLKSIDKQIIQKSFTIPYKNIPISYNIFNTLDYGKLQEKMMCSLFLNSNYYITCMNFDTLNYEKLNSNLQERIKIETTISSQEICEEIYQLDENFILLCFSQFNLKQYSVSLLGETILLLEYDVYFQVKDQCKKKIQKVVGKNQFRIAFFQCSLWKIILINHQEIVIILESKMNEGNPQLKEFFKIDDVQYCEGGKSSKSLGIQSLYIIEGNNYLQLRWESNQKITQELFKSENQILKLILLQTCQNVMLITFQEENNQQPFQKLDNEIVLNQAFEVEQIHFFYNFLFLQNSTELIIRIDKHLIQKYEILNTKLIYMESYNLFYQIDKTKELVIFYRYFPISAFLQPTQKFVYFIQLFDGFEKQYMMCIKIQYEKTLDKKTPELINLQIEKDCSQNQKTILSIIDFQSFQQMPFSLYNNYTNGVINLSIWNEGEQENLCYYQLRMYNFKGKIQLKSMYTFTHVVFQVEDSIYFHDCQLKKIQLILNTHQYEVFQNQEYYFLIDKNKKQIRMIYIFSGITQQIVTHSNIEIISAQQIGEQIIIYIKNQQSPILISKKILEKIQNNYLSQKLKQPGEILYYQEIKSYKFIQYQNIFAFEQNEEVRCFQFSDVQIVQIKIYVKAHFMIFAVQIQTNSILLYHFDQFEIHQFHNYTLEDYSFSSPLRYFNCEKLIFLHNFIEFYSFQNQLDGYQRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y5V6</accession>
    <name>A0A8S1Y5V6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1500112</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000150">
        <property type="protein sequence ID" value="CAD8208378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y5V6"/>
    <dbReference type="OrthoDB" id="297107at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8208378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="179" mass="20801" checksum="629F2E526E47FCCB" modified="2022-10-12" version="1">MQKTWLEIPKRPLDINFDIKTIRNQVKDKLVPPFQYNTNSGSKQFNSFRKESLPTIPSFDNLQAKIRDFSYTRLQSTSSLQSKITSTKKYDDQSPKKTISSMGFRNNIQSYRQQSIIQQPIELNYRTQQKRSLVENLQLNEIVSLRNKIESSYSNRNSLSANYVSELVKLAQAITNALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y667</accession>
    <name>A0A8S1Y667_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1520116</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000152">
        <property type="protein sequence ID" value="CAD8208951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y667"/>
    <dbReference type="OrthoDB" id="290816at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="19"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8208951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="549" mass="64450" checksum="BDEA1FE39A7C6BB5" modified="2022-10-12" version="1">MLLSSQNLDTKTIAEKYVLLKKKQIGTGAFATVFEGFVKGNAQEKVAIKILQSLPKDSTNKTIETLKILYQREREIHKQIDSENVVKMIDVIQCDQSTYLILEFCEEGNLNKILNRQPKRRFNQERAYQIFCQIVEGYKSLYKLKTLHRDLKPENILFSKGVAKIADFGFAKIIEEMDLAADQTVVGTLLYQAPEMMVSSKYSSKVDIWSLGVIFYEMLYGTTPFNEQHPNKLHKKITTEPLKFPQDVQINESYINLLKKMLKVDPEMRIRWDEIFLLLSKDQNNQIITDENFSVATTHMSDSDRFQTNYKKVEQQINKIDEIKDYFDYVQRIFSFSNNNTARLFFTLPDKLQISPSQQLLFSITSTKYLLNQYNFYIQILLGQLPPSEINFKENDIKTFQSDQAKYYSVLNYLKTNHETMNQFYNEKLLPQFIKLKQADNTNQLIKIIDELVQTNQRNKKSWDSFNKVYMYFIAEITKSIKSKQITDEATLRQIYTLQINMFFSLHPLKFIQNEFEPKNLENELIQSSTQKLENKVTELNIIYSAVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8E9</accession>
    <name>A0A8S1Y8E9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Arginine kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1560054</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the ATP:guanido phosphotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000156">
        <property type="protein sequence ID" value="CAD8209820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y8E9"/>
    <dbReference type="OrthoDB" id="430219at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004111">
        <property type="term" value="F:creatine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046314">
        <property type="term" value="P:phosphocreatine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07931">
        <property type="entry name" value="eukaryotic_phosphagen_kinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000006">
        <property type="entry name" value="Arginine kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.135.10:FF:000008">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000749">
        <property type="entry name" value="ATP-guanido_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022414">
        <property type="entry name" value="ATP-guanido_PTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022413">
        <property type="entry name" value="ATP-guanido_PTrfase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11547">
        <property type="entry name" value="ARGININE OR CREATINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11547:SF64">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_51, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00217">
        <property type="entry name" value="ATP-gua_Ptrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02807">
        <property type="entry name" value="ATP-gua_PtransN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51510">
        <property type="entry name" value="PHOSPHAGEN_KINASE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51509">
        <property type="entry name" value="PHOSPHAGEN_KINASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphagen kinase N-terminal" evidence="5">
        <location>
            <begin position="21"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphagen kinase C-terminal" evidence="6">
        <location>
            <begin position="134"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="137"/>
            <end position="141"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="301"/>
            <end position="305"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="333"/>
            <end position="338"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8209820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="394" mass="44987" checksum="E5FE5550C5B38B6F" modified="2022-10-12" version="1">MSQKIFEKKDVRKLYLDDQGNVDYTKIQARWEEFKKNKVSLANKHFTQQVVEKAKTLSQEDQQRLLDIMIEGLTYNDSSVGIAATRPEDYDLFLFYLEPLIREYHKIEGQTKQEHDWNIPVGEYVLTNIDPKLEKVSMRARVARNVVGYSLPSSMDKDERIKFENQMVEVFEKFGFPGKYHSLTPGHKNFISDQEADVLRNKHFLFINMTHDNCLMSNGVASDWPYGRGIWISEDETKMVWVGEEDHLRIISIVQGNDLGKVDQSLNELLTGIEKSGLKFAEHPTYGIITTCPTNMGTGKRQSILGKFPNITKGGKDEANLKDIAKSLGLQARGIGGEHSNMDSEGTADISPSARFGVTEAIVTKRLYEGLIKLYEIERNETNEKKRQNCCNTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YBA7</accession>
    <name>A0A8S1YBA7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1630041</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000163">
        <property type="protein sequence ID" value="CAD8211355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YBA7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8211355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="37" mass="4411" checksum="A8A819B486C67DA5" modified="2022-10-12" version="1">MSKAKYKKDQRCKGCVLQRQMNGSRLILFKPNFSHLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YBD1</accession>
    <name>A0A8S1YBD1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1650039</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000165">
        <property type="protein sequence ID" value="CAD8211786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YBD1"/>
    <dbReference type="OrthoDB" id="1939479at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019784">
        <property type="term" value="F:deNEDDylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000338">
        <property type="term" value="P:protein deneddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044613">
        <property type="entry name" value="Nep1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46468">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46468:SF1">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="4">
        <location>
            <begin position="173"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8211786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="385" mass="46374" checksum="D81BB71A7EE1B03D" modified="2022-10-12" version="1">MKSIIDNILIQNKMQFIEINTKHHQFDRLILQTHIRFYCQSNSYIKHESVERIKGKCYKKLFDFNKNPKFLIQEGIFRINIKKEEYLLDGEGIEYCGKSEIIKIQGVFIQGELQEIESKQKSKSFNLQENSQSRAKIIIIMDQNNQIIRSKSLENDNEKLREMYQGNRILYNQSLSDRDVNILRNNSKEWLTSSLIDSFVEYLNQKSEIKVNMLQLEERMKIPRILFIPSYVTSSFGLYEDETTYMKKAEGLLQTELLFYKELNYEMKQIYTKIGFPVNINNMHWVFLLFDLRKEEILLFDSLKSTKKKRATPGYLSKAIGRFLNLDIRKCYLMNYIQQKDNYSCGYFVSSFMEFEYKLQFNKNCRYFYDENKMKMKFRQLMRFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YBI1</accession>
    <name>A0A8S1YBI1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1580034</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000158">
        <property type="protein sequence ID" value="CAD8210157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YBI1"/>
    <dbReference type="OrthoDB" id="277011at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000121">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="2">
        <location>
            <begin position="220"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8210157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="421" mass="48466" checksum="26E68938711C7AF6" modified="2022-10-12" version="1">MDFNISNCNLLQKNQPNHQSSPKKLSNSEVNAAIQKYFQIVKRKEGKNPNPIISNAQSSKILGQLVTKVSPSLDIHVPIKPPTPLGTQSSRPSSEKKVYHRPQQSVGQSKDLNQYKLLTTLFQKPKTQSQQVTPKCSGKGSVSVNKYFQSTKKLQGEFQQYYLTRVKNAFSKPLRDDYFSRMYREHFLQTYQGIYITQYLQPVDPNDLQNKQVHLKQKECYTNKITIVFDLDETLVHCNESIAMPSDIILSIQVSPQEKVKAGINIRPGAVKLLELLVNDFELIVFTASHPCYAQKVIEYLDPQKKLFSHSLYRDNCIMTTGGMYTKDLRIIDRSLSQLVLVDNASYSFGWQLDNGIPIIPFYDNKEDRELESLLKYLKGMQGCKDVREYNRNHLKFQFFQDPSGPGLVFEKLFEQKMEIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YCE5</accession>
    <name>A0A8S1YCE5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Acyl carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T1660113</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Carrier of the growing fatty acid chain in fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl carrier protein (ACP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000166">
        <property type="protein sequence ID" value="CAD8212146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YCE5"/>
    <dbReference type="OrthoDB" id="448946at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000035">
        <property type="term" value="F:acyl binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000036">
        <property type="term" value="F:acyl carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003231">
        <property type="entry name" value="ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863">
        <property type="entry name" value="ACYL CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863:SF28">
        <property type="entry name" value="ACYL CARRIER PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="11 12" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 12" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 12" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Carrier" evidence="13">
        <location>
            <begin position="41"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8212146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="119" mass="13865" checksum="B4461C2EEF4AD4EC" modified="2022-10-12" version="1">MNRRLVQIFNKQFSQVPKFFTSNEQPMVLRNEKSGYYVNPEAVARRMIKVISLHDDVKNPSAITLQSTWTDIGLSDMAYVEVMVEVEREFEIEFPDTDVECFRTVNDAVEYVARSFFAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YCH8</accession>
    <name>A0A8S1YCH8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1550032</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000155">
        <property type="protein sequence ID" value="CAD8209542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="422728at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1681"/>
            <end position="1700"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8209542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1733" mass="202923" checksum="4937F234B124893D" modified="2022-10-12" version="1">MLILILLFQLTLSQQYDQEILELEMEKQYEFYGKVPGASTSVLYPVHFYKFHVENVIPNEDICVVLRALHDQSSYPSLYISKVNQYPTIYDELCGNKGMDVCIIEEENLIANTTYYLGVYCLQDCDYELTIHYEEEEVLQLGEAVIVRFDNETESILKIGLPNSMDGIDRILIRAHYIEDRNTILNESFHFYINEGNETPSPIQFDYEAEGIWRIGKGIVIYKNSTNLRGNLTVLMLGVPNTRLYFISTVYIGIREIGLFERIDDLVIERKTNYYKLTIQQEYLEFLDGNSLSFDIHPFEGNPDVYISPCYKGECTLDYKQYPWQSQLDLGYESITISRNNRLINGNGSEFIIAINGVDEFSSYSFLAYLSNYYSRILSIYSFESGFLVKNELAEYNIGIFNELNQTFTITAKYPTGHGMIIFKKCMKNNTELSKVTNSYAYLINAGPDNFYNCSITKEQIEKIQIGQILKSGSSELYSAENTLQFYYNKSDCESNVTMLNYENIQTNCQYVVGVYSNVEYMNYQIFIKGQEQHVELTESTTHRSFLMEYNTDLYSFTINEQQEIIEVVFQITAITGEYDAYASRLYDKPNSTFYDRYANMELDVLQYKVENETQSLSGQYYIRIIAKALLRYSITPIVYRKSHEDINYIHLTESISYTHLQTKIDQATYFNFEYRQKGPLYIHLNGIYGYFICYIIGSNGIIGDKYPNNSRYDFAFRSSQHTFIIENPQQYYYIQVNSISLGLNESYEIQYTSSSSLMELFYGEPLITQLDNQHQSFYYYQSPYALEKLFIIKTQITEINEDNNLQIYISLNNKFPNHQNYDYTIYYSSTYLTLSNITENTIVYIGVYSIGYNEYSLLIRGVSGIIELLDNTVQQVPIPSFEQYQSSNLYFLVPKNINNTIQIQAYTQFSEIILFCTIQDYAVVQKDLRNNNHLIFPNSSSYDIKEESNASISNKLLIINREQLEICQNYIQGCVLLINVKLDLQSLFSYIQGKSYNNISTVNGEDGYFNIMISTQYSIIKNGEMLIGHSGENIMSYYYFYVDKPVEFIQIAVRPIDNCDPDIYVNKLINNTISYPTEDTNTYKSMSYKSDILIIKESDNIGSYMIGIPGFKKKCTYELLLNFADIELYYISNGQFVTHQINQTVYYFYQHIQNVSFRIMIQGINNIDVAINTYSSYSDDNDGLYEVLPYYGTDDIQILQEHNDNLFGGIIQITQSNKHFCYYCTYIIALKPLKNTDIQLLIAYNSIALDIEYGRIYYDQCIESCKYQVKSGDLNIYVYSQNIELQFYDQDIFIYSMNLSNSQHIIPINGSYTLKIVNSNLIASYWLSLQNEQNLINLHLGKSYKGNNTIEQNVNQFTFTPITIEQWYTLIVNSNSKIKVELFYLSKNITEFILINPIQEWSIRESQLELQYQLPSVDYYYQINLYCQSQYSITLNLEGLKYIDSNQHYIEQIKSTQIYNIYGIKGQELLIEKIDCLGQTESNISSYQFKNSSDIYFNVTPTLFANYTKNGLSIKKNIYSLIPHLQYSHDIWYFNKTIAYQIKQEDQTLTITVDTMKRNKTGILDLRMLIYQLHYSNQEQFLNAFGCQMDIESLKYYTNSQLFHIKQMQTIKEENDIVQFEIPIPQDEKILYGLIVFQAFYQGYDTPYTYFYKTNLIYNETQTMFNINSKDQKQNINRDYIIIMVIVTCLIMLMLFLGIRKIIYRRKYVQQKKLEKMKDVKVDEEWEQVKGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YDP2</accession>
    <name>A0A8S1YDP2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1650086</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000165">
        <property type="protein sequence ID" value="CAD8211879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YDP2"/>
    <dbReference type="OrthoDB" id="4977at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005869">
        <property type="term" value="C:dynactin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028133">
        <property type="entry name" value="Dynamitin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04912">
        <property type="entry name" value="Dynamitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8211879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="243" mass="28096" checksum="75A34A2413B3660A" modified="2022-10-12" version="1">MQSDYYESDNIEVIPEEHNDRMAPKEKVTLKEQIEFLQQQTTLLKQSMELEQELQVDNPIAYLNAIENNLESLKVTNLQQRIQCLAILGSGAEKCVTLKCILPQSDNLKRFQQLEKQVNLLETIVGQKIYPYNTTIMQDLNEVQQIINSIQGENKDIKLGFTQSLPQIDKINEIFRQVQLLVGIADLLPNIITRLEQLKNAHDQSIQYQNQLKELQIKHTKVLDMISNNRTRIKVLCAKLAQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YJS0</accession>
    <name>A0A8S1YJS0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ADP-ribosylation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1960006</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000196">
        <property type="protein sequence ID" value="CAD8214099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YJS0"/>
    <dbReference type="OrthoDB" id="284243at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00878">
        <property type="entry name" value="Arf_Arl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001915">
        <property type="entry name" value="ADP-ribosylation factor 6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="29"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="128"/>
            <end position="131"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8214099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="181" mass="20613" checksum="69CAC4D4CDE2091E" modified="2022-10-12" version="1">MGCLSSSQKEEDVSVALQGQKKLKIVIIGLEGSGKTAILQYLRNRKFTETQPTTGLNVETIQYKSRFYLIFDVGGKVRTLWSHYYENLDGLVFVVDSTNQEKQQEILKELKKLSDDVKTKIVLLIYLNKSDLPNSQFMELGVDIQQSNSDVIMQRCSAKSGEGIWEGIDKMNRLLDHKFQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YND9</accession>
    <name>A0A8S1YND9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">26S proteasome regulatory subunit Rpn7 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1700064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000170">
        <property type="protein sequence ID" value="CAD8212714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YND9"/>
    <dbReference type="OrthoDB" id="1452at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045135">
        <property type="entry name" value="Rpn7_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10602">
        <property type="entry name" value="RPN7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="26S proteasome regulatory subunit Rpn7 N-terminal" evidence="1">
        <location>
            <begin position="45"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8212714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="118" mass="14228" checksum="940D2C9B01D1982A" modified="2022-10-12" version="1">MLKQGMQSQRRQHCVKSKKNLKMPSKLTLKPYPNLLELVKNGCSFSYILNHLKQRNLEKLKDHLNQQQAQQNQGGDWERKNKLKIYERIYGLMIRDIQKLANYFQNLFNCQFIRNYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YNI0</accession>
    <name>A0A8S1YNI0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HTH La-type RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1720076</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000172">
        <property type="protein sequence ID" value="CAD8213012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YNI0"/>
    <dbReference type="OrthoDB" id="307741at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006630">
        <property type="entry name" value="La_HTH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05383">
        <property type="entry name" value="La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50961">
        <property type="entry name" value="HTH_LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="HTH La-type RNA-binding" evidence="5">
        <location>
            <begin position="391"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="240"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="370"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="240"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8213012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="489" mass="58463" checksum="88672D3BCC56BB1F" modified="2022-10-12" version="1">MLVTLIYILQYNNQRFFLKIGVFIIKRKIQIKFVQLLLFSLYQSQEKFSLYICYIRFIRFIKEGFSSINKGDGFLLLLNKGQFSILTQRGIKILTYVVLFYNCYIISLNKITQKRQKYNRSIKQVNILSIITQQNQQSSSEENDKKLQKNNSYFKIKTSKSSIQNLIQLLYLINIQRKLYKQINKKQGQNKVNHFRIRVVFQRSSNQKNIISKHNVICNLIWRGRNKKQAKEWRKIFNEGSQQSTHKQISQQNNLSTIQSNSHKNSQENTQEQQLQQIGSIFSTIQLNEYINLPVQYIKDDSTLIIKMTKYFLRKVEKNCQQDKKLIIQINRNQTLIIYYRELKELKIHFLAIESQTRKQIIKFLSQDSDKQQQVSLQQLNNKIDDIQQQEKEFVIDPFGIGQQINYYYSDKNYSKDNFILQHSAKDPEKFMKFKLLLNFPKVKLKIKSEEELLKIVELFLIKTKEDLVNFESKKNQEGLYMIRKKQIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YNS9</accession>
    <name>A0A8S1YNS9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1710056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000171">
        <property type="protein sequence ID" value="CAD8212844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YNS9"/>
    <dbReference type="OrthoDB" id="298721at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="700"/>
            <end position="731"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8212844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="791" mass="94553" checksum="909EFC0B49A9748E" modified="2022-10-12" version="1">MGCMNASGYISGPIEPFYQSNKSNFEAFLIEQLTLLGFQESQIVISKTIEIKFIPYLKELQTSIQQDIHSPFHNNIYLIPVLTNQLREIYIKTSEFLIRFQDYKLNTLINKVGLKYQALLRVGDLFPKQLNLRFYNNQISKIQIDPNIIIIVINQIDIGYALLQFIKDLNQIYLLVFRYDENDNQNINLFIETNSLQNCSVIIGVDDKEDRYNTIINLYTKVIKKQKKNQNSDSHSRILTQFLNQEDQEQFWDDGTLIFNFLNSTQTHISTNTSIITIQNYKIITINEFNTKDQQFHTQKSLQDKKHIDQYIYKSIKQIMIKELIQALQLKSSQFSQYFKLKLSTQKIYKDLKLQQKIYMSSQLKLQNVNEIESKTQEILTIIKKASYDTLIIKQSSKFQLPNKIRIYQILQQIFIDQDINQVQIMINSILHIQWSLGDEIINSQFNIKNQNYQNYKYKWKMIKFYDNFKIINLAQPKKQQVYFQAKQLIKCLIRKSDQEIVNKITNQIILDKWNLNEEYYPICDVYQISFINSTSVDETSQYQEKLDKIVVSQENQYLIIFLMDYQDPQRISQLDQIISLPLQLDIKLIILSNSEFDNDNFEQLLNKKKIEKRIYQKNIQFWFPIQSQINEMPFSQYLEIFYNLNQKENVIIINNKQLLCHKITGFHLITMLQERNYQDDDKKQYNYYQSKNQQRIVNNEQLKKVRQQLKQNIEKEIKQQQQLSLQNEQDHQTNMIFDFKWAKEKTIKLDGQQKVSVVSKQYLKPIFNNNSGIQFEIFQQIISNKQYDFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RZN1</accession>
    <name>A0A8S1RZN1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0020414</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RZN1"/>
    <dbReference type="OrthoDB" id="296708at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8133456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="218" mass="24966" checksum="39374D03638E86FA" modified="2022-10-12" version="1">MGSSQCCTVNTNEQASELAIGMKETSQSSKGPNRSSDGKKNKNKNQPLSPMEKQKVTHGQFRIDDPQTPAFADNKSIHSKQESEQQSNKDIQLVAEIVMQLDDIHQKIDQGNEDIFISQTSLKKKRKNDSPLCEFQPKNSLKSFDSKRLNKLKSISEHKQNKEIINKEELESQRSQSADNRSVKSILKQEMKYSQYKKGQFSNNDIHSQKRVHFQLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S1M3</accession>
    <name>A0A8S1S1M3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0030157</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S1M3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="168"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8134148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="227" mass="26682" checksum="F3E8E0504BD9020B" modified="2022-10-12" version="1">MTSIFQLDQEYRILFEQIMGMLDQLSSISLEFVSSKLTFLEKYSQTLKTSQMDARQHRNNQIKNLIEYVKMNSKSKVRSMIHSPFNRTFHHHNSLDMQDIHINENISVIPNSCVSQKCLSDTKLFHTKTIKNILQKIVSKKNDSPQKTFTDPLLKLLENALSLLETHQQYYQKDYNELKKEYDELLQNNPQIMAKVSLQNKSIKIKENLNSLIETQKSFKSYLNSIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S233</accession>
    <name>A0A8S1S233_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TORTIFOLIA1/SINE1-2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0030202</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S233"/>
    <dbReference type="OrthoDB" id="298726at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001544">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033337">
        <property type="entry name" value="TORTIFOLIA1/SINE1-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057600">
        <property type="entry name" value="TORTIFOLIA1/SINE1-2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31355">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN TORTIFOLIA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31355:SF7">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN TORTIFOLIA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24714">
        <property type="entry name" value="TOR1L1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TORTIFOLIA1/SINE1-2 N-terminal" evidence="3">
        <location>
            <begin position="21"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="610"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="469"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="610"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="636"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8134236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="775" mass="90373" checksum="CABB1CE515B0969D" modified="2022-10-12" version="1">MNSSALNKSRRQQSSYDSNSQLRTILLASLAKLHSQSTAQTGFSELRQLIGQLSQNQFVILTNLLDSNDLSKQAKKDYIKVFGLLSEIRGNDIQEQIPRVVQIINKRISEGDSSFLQVISDTFANINEFTIQTSPNQYDLFFQITELLQTNFIHNNKVAQLISAASLSRLIKTSKSQLLELVCKQYTQRTLEILKSPHCQTQQGLLESLLSIILSVEEIFEPNLEDCLNTICNCLQSSEWNSRKIAVDILYTLSVIFPHYFRANQQYSNKISELRFDKIKHVRDAASIAISSLKESQILQQQSQREHKSLFKQNANKSFFEKKHDIQIVENKPKEINLQKQQIFGYDPQLKRERFYSNEKRQSPFQILTESNVLASPHQEQIQQMESNNKINTFGSSSKKRQVNLSQEIQTSNQLINSITPKKQQTDLIKLYDSAKSKQLAYYEQIIAKKIEESEQITKQEFSKINSRIDKIELLLEKMNETIENKFQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQLQCLKKKVQPVEIYEDQMIPCLLHQENQQQKDQLMYDSLNEANQEQLQNSKMKDLFFFGQKCELITESKLGESNFEFNSRKMSEADCKERENHQRSTSEASPQRIRDQSILTNDSKQTKEQTSPLPQLKDPKIQEQQKIMQRVEEQLKKENLNEAYCTALTSLDDQIIISTMMKTGPCTERLDSTQVEFLLHKLRTLDWIENALQHHLARMPAILLKSISTQLNNIQTTEQVRRIQNLIDKKQTLTSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S2E3</accession>
    <name>A0A8S1S2E3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0020471</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S2E3"/>
    <dbReference type="OrthoDB" id="303233at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="31"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8133570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="311" mass="37897" checksum="86AB7E36171A6CA8" modified="2022-10-12" version="1">MASQKFMEFLSSNDKLRMNDMKLIEQEQYYINQLKDQREKMELLKIEFLKKDHVILELQGHLEQLQDECEQLREIYQEHVQNQENEFYRFNKLQVEKDNLIKDNEELKDVIQHLRNENETLNSLQFKNDDQQRQLQEQLVLLAEENARLKQEIAKLDELLFTYEPLKEENEKLNEKLRYYKHEKHKLSQELKQGKQEINKQIEEFKSGYVKDLKNEIQRLQEYKDQYQSLKIQIQDLEQKYIEVINENNDLKLEIKSLYEKEESEIKIKSEIDRVRQDLMSVRYQETLKLNELFNGMISLSSLANSRDHFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SGG1</accession>
    <name>A0A8S1SGG1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0080117</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SGG1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8139475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="104" mass="12533" checksum="3B8EB5E4F464D4A1" modified="2022-10-12" version="1">MKSIQIHSMNLNKQIDQQLVQLQESIGKKLQLQKKLLYKNCKILIMNLLILLKIRNLTSKIINDTYILYLKIQERYQQILYKWNNQCKLLDLNLILQIYSKDNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SGN3</accession>
    <name>A0A8S1SGN3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0070419</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8139000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SGN3"/>
    <dbReference type="OrthoDB" id="294945at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8139000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="336" mass="40341" checksum="4DB0660380675095" modified="2022-10-12" version="1">MSLIVRNISSIREEWRQLMKKKIKKTKRKNQYTIPYKFDDDIQNTKQKDDSLLIVQNIPTTIPNNNIFRLKTNIQFSLNINKDYSLGSLFVDPHSNNYKIIRIDKRKNLFILTLDQIDRPITPPEVLYSIFRDRNTELAQAFMQYNNRNLEPTKCKLYTIQEMLDFEQKIQGMPYAVYVNIYKDGIWKTFQKIFNQELLQILAISEEMLFHYCNETKLIPISAYFDQNDEQLNVFYKIFKGIVGQQSIKREYMNYNGEQFTASLIVQCFFIYDEEKDMLFDYTYFVTDCDKKWLSQERVRQNELDYFNIKPNSCSTEESSYQEPLKRCGFKILNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SPM2</accession>
    <name>A0A8S1SPM2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Palmitoyltransferase</fullName>
            <ecNumber evidence="8">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0100047</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family. PFA5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SPM2"/>
    <dbReference type="OrthoDB" id="288061at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF23">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="180"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="217"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="134"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8141309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="286" mass="34140" checksum="84B87640211BB24A" modified="2022-10-12" version="1">MSLLPTFILFGVLLFTYSIYYFTNLKPNFNGFTYEIIIMHLIILLFLVSIFRAMFLQPGVISTEIIDETWKQWEIFQQQEKERLTEQKQRRSQKSVKTFKTENDEDRSVVNLDAEEIIEEEDNITKDLNQKGVQKRFCKKCCIPKPPRAHHCSQCNTCWLRMDHHCQWINNCVAKDNYKMFFCMIFYASCLLIWVTISQYKVFENVIEYDVSDLELYIIVLHFYFVFLLAVLISGFFIFHIYLTIQNKTTLEQLEDKPDKRKYDQGIWFNCQSALGSNILVWLLPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SQ65</accession>
    <name>A0A8S1SQ65_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0100403</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SQ65"/>
    <dbReference type="OrthoDB" id="303637at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="283"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="185"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="283"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8141978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="311" mass="36904" checksum="81878C9F69B06FF9" modified="2022-10-12" version="1">MLNLRELQELRQLNDERKALTDRYGVGYNQPLSARNEYNGKQTLSAWDPITNKPQTLIVNNGSDPIVLDHNIPRQEITKNIVFQKGFPFQNNQPLTKNYQVQIQDIPTSISKIQTSKLLPQNYMHEYHHRQRPYQLKSVEEILRPWKAKEIMYNRELKNLQEQINKGNYTMTDKRTLKVDNVAKKLELQNEVQKLEAILKNKDEEITRLKIRSQEIEEKYNELSEENSTELNKYQKELEEWKKKFKDLNNLYHECEEECTMKETEIESLKKRKLSVIKTQTVQKVGTKPQKNMDSPRAIISTVSDKDSDYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SS19</accession>
    <name>A0A8S1SS19_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0120020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SS19"/>
    <dbReference type="OrthoDB" id="443402at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="3"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="154"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="334"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8143063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="515" mass="61127" checksum="EF03D83A0E80ED54" modified="2022-10-12" version="1">MEIEKWKTQCRYLQNELDSMKQQLQDAKDLIRLNKEQLKICYNPTQFDQSSECKAALVLLKFVQEENEVLMKKIEQLSDERNLAFDKAFISEQINESNQRVESSLITSLNRTITELKKKLNEQVKMSNEIMGTIYLEKHLAQPEQLTLNFNNVIQQMNSMLLKQKLQIEELIEQKNDLAQLNFSLSNEILKIRTQRFTPRNQTKTFFSQNEDPSKFINNLVQLQKRLFFDDDSQDEKMHMQQSAVFSPLFSPTLPQKVKRQQKSQIPKDLKQEPEKHIPKLDLTKAQQIQQFNIKRMQLLANKLANEGVEQKIYMLQDELLRTKNKYHAQLVLNQQLDLQLTELNLQIMELQNVNETLIKSNQMYEEKWSSIYQQFSFYKEFYLKHKDQIQHNIQSFATSDDQQQFSAFEFEKVLNSSKIYNKNLSQKNNNNNNPAISIINYQDDTIQQSSCRLPTDRQEEVNKFLTQQYKCCLMMMAKEVISLDYQHIKPVQTNLNKVKIKRSYSNTIEYFPVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SSI3</accession>
    <name>A0A8S1SSI3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tim10-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0110065</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SSI3"/>
    <dbReference type="OrthoDB" id="1551503at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050673">
        <property type="entry name" value="Mito_inner_translocase_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13172">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0811">Translocation</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Tim10-like" evidence="4">
        <location>
            <begin position="27"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8142289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="95" mass="10928" checksum="AFED93583E7F92B6" modified="2022-10-12" version="1">MHGPDISDLDMPDLTLYPSTTGYSEQKRCFKQCIKDMSIPYLNIDEESCSKNCLSKYLQAQIFLSQKMTNTLTQLVTQDPSIKIAFKKPYKLPEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SX91</accession>
    <name>A0A8S1SX91_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0150271</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SX91"/>
    <dbReference type="OrthoDB" id="10251809at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14733">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093:SF18">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="383"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8146291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="541" mass="64100" checksum="508B6023C097DFDD" modified="2022-10-12" version="1">MNRRLSSEQDNLRIKRALMRQQINQSHRVDREFEIDERFHSVSMYIKHDELEIDDCEYYDHQTWERLDRFDQVVPNQRNNCSWVIYEDFLYIFGGFTFNGRLDDVHRYSFGSNQWQRLNTSGQKPSARENNGAIQYKGHMYVFGGCDGLLWLNDFYSLNLKTLQWKKIEPTGQCPSERFGIACGAYQTKMLIFGGCDGNHYLNDAYVWDFEEQVWNKLQLIGDIPSARSCPSFSTLNNQIYIFGGFDGVNRLNDFYKINIFTGKVKRISQHGTIPCPRYFHTSEVYQNKLLLFGGFNGQARLNDLYEFEFGSKTWKKLEVHEPPKGRSSMVFQLYNDSLYVFGGYDGDELLSDIYKLEFKNAQVPRSSFIQDLRSLINNPQMSDVVFIVEDHQIYANRCILGARSEHFQTLFFEEFRDKEQIYIEITECCYQTFMDMLLYIYTDQLDICLNTNRLLSLIILSDQYLMQRLKYLCEEQLIRKINCNNVIELILFSKKYNCRLLRIQTMKQLVDNITTIKRRKDFIKLAQEPEILLEIIQKKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SXE1</accession>
    <name>A0A8S1SXE1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0150291</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SXE1"/>
    <dbReference type="OrthoDB" id="298034at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8146331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="625" mass="72870" checksum="4897E1AC67C76739" modified="2022-10-12" version="1">MEQPNYKSVAEYQAHLYQKNVAYQIEQFLCQKLGQEAELNLQCTFSRIPVTIPIRHQNVASIKCIFELELWLQFFESKLKNQNNFSCPGCKQYVYFQDFGIDFTLYHILTAKKKLEQGGQKLLSEKVIYTFNNGKTTYYAISKLDKQTKIKIPGSSPIIRLPILKNSLKVQDLNPGQQRIHEQINYTISQIQQYITQRLFQGKQQGQQINQMKQALRGKLDQLKQTFRQSQNLIELGVKSLETQFIFGLKKLQKDGKNVGSVLIVYYPHLGIWADYEIKTTPEKHFQFEYQLYVKEQGLEKENIIYIIGGRVQNLVPTNQFVRIRFPKDPFSQDQQASIDYLPNLPNAGFNYLGGCYNNNVYIFCGQSRTPDQSGIIIDKIFNVGYVFKNGQWNQLNQKVEKRYDGSCTIINHQKYDKCLLLYGGAEQLPDGRMGYNIKEQQHIVQVFQFKQEKFIGNGFKLKFSDNNQDSYQKSMLSSPVFSVPFGAHSELLIAGDFLKKNWKEKREVYIFDWEDGTIKINLLQTQALEQQLLSHIKRNYNYSGAEFLQPIQDFEGAFLFGNYFTIHQEETYIDQKTTIQNFQLFEYKVANGQVTTRPYMQRDVSIQLAIEALQKLANQENKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SYM7</accession>
    <name>A0A8S1SYM7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0120306</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SYM7"/>
    <dbReference type="OrthoDB" id="283847at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8143592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="181" mass="21658" checksum="4F775057D9D3015B" modified="2022-10-12" version="1">MNQLRNQRGTETDEVYIDPERGIAINARQMTTEQHFKYYKSSFSTLRPDLTEYEYEAFAKRLRVGESFLNHMRVFLNHESGRVTNLYPVSARLDKMFNYQNQYFHLRPPFILGHRSNANRNWADASKVVNYVEKQLLKITKYGLDYPNYYAPKTENELKQREDDIYERFIKEMRKPPIAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TFK5</accession>
    <name>A0A8S1TFK5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0200248</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TFK5"/>
    <dbReference type="OrthoDB" id="292767at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8150414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="193" mass="22183" checksum="3627F99BCE8D826C" modified="2022-10-12" version="1">MGSAQCCKKNVQDEDVQEVVILEDSNQQSAKLQDVQQAKHSYKKTEECGTPAFPELRSQQSKRSEQCQSLKEIQFVGQVDKDEQILKSTCTLGRKRQKNDSPIVAFEAKNSLKSFDSNRLKKLQSVTEQQLNLKNISSPRDEDSFMSDSQRGSRQPDEKSAKSILKQEMKYSRFRNQQNQVDSTLRKVQFNLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TGU2</accession>
    <name>A0A8S1TGU2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0200254</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TGU2"/>
    <dbReference type="OrthoDB" id="302624at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8150426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="86" mass="10047" checksum="B1A69D510EDC456A" modified="2022-10-12" version="1">MGCGSIKSVQETNQPIQMKQKSLKLKTSSTLPEEIKKKVFRNKDGKLFYIVEEDNKYIQVPLLNSAEQNSLLNRRRQMRTQSDQLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TID9</accession>
    <name>A0A8S1TID9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0200384</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TID9"/>
    <dbReference type="OrthoDB" id="5979581at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000616">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="14"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="345"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="345"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="360"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="374"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8150639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="460" mass="53994" checksum="9FC0A599065297E9" modified="2022-10-12" version="1">MSRDSQQKVFLNRFVVKKKISSGAFGVVFLVFDKQTNQEHALKLEKEDNEEMRSLEREVEILKQLNEAEGTPKLIWFGTEDEFNLMVIQLLGRDLSFYFRQQKKFSLKCSIQIAYDCVQILRNIHNRNVIHRDLKPENILMSKDNESLYIVDFGISKVYCVQGEHMPFRKDKTFMGTPRYASIAAHLGHEIARKDDLESLFYVILYFLRGSLPWQNLPVSENERTKAVGDIKQNIDLNDLCANQPYELVEIMQYIKNLSFSDEPNYQYILQMLNQIAENNQFILDGIYDWTEGLMKSTKKFSSLESKRSFEFNKIKEHSPGPIMKSSSKLSNQIIWTINEQPQHGQFPNSQQHLLQPPDPNKKPGQRSDFRHLSNSSSNSNVGSFSSMKIKYLPSQIEIEKPSLFPKWEKTYIQQDSQYQEQMDENNFEIPLEIKYKNYTKIQLLEIYNNGFNKFLENTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TJP5</accession>
    <name>A0A8S1TJP5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0210320</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TJP5"/>
    <dbReference type="OrthoDB" id="287615at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16449">
        <property type="entry name" value="RING-HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23327">
        <property type="entry name" value="RING FINGER PROTEIN 127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23327:SF51">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR OF YEAST FORM ADHERENCE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="7"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="86"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8151372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="409" mass="48048" checksum="AF6770056AA0C3B7" modified="2022-10-12" version="1">MKFILQCSICLQNLKNPVSLSCGHTFCQNCIQHSFDTQEFCACPLCRQPALLSNNKTDELLPIVQQIYEQEGTQSLLNEFPSLIICLCCGLTPVNPVVLSCQHMFCQKCIEESLKEELLCPACSDYCFNIKVNTNKKYKDLIDWYLKEFKIEEEQEIDQVQQIESIFGIPIFHFDQSVIIYGQTDFQFLEFRYQEMIKMVSSGSGNFIVSNDLINGDLVQIKSIKKTNKGFQVQVDGISRIRIKQVYTYIDGVKTQYQNWNTQQLWLCQCEYVRDQIFKENCFQQYKYIVATLEKLYQNIKLEIRDIFQTFMKKITTLSNSESSLILLASLNNNFEQEYYSTDIEKRIQQIQQHFAILEQKIKGLYDKDDQIQHAKLKYDSIEIVQYPEFDVTTQYFLNLFNIKKVLCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TKY7</accession>
    <name>A0A8S1TKY7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0240303</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052394">
        <property type="entry name" value="LRR-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8153595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1580" mass="188105" checksum="33680571E7DEF5F6" modified="2022-10-12" version="1">MFFEKLRIYNTKEKLIERIIQYKREEELRRNSPIREKIKRTISTCQTQRTAFNCNNRISVSPKIQCSLDIEKIKMPFSQERDGHLTRVTEIPVRQHLATPQPPQKSIIDHLNELEEEKMIRELLDNYKPELFDDMMENDIQEIERFNEDAHANSMKQFSYQDYSPQRMNMIKKHQHHHHKSNSTRNNNNNKYDQQRQLYLQQLEFKGDIYQEQNTDRGRYLKRFRLANKNLNKVCQEDFRKVVKSQNKLEDFAQFYKQPPNLYMQLNKSSHQQSIFQSGLGLVQQCNHIQIANASGQIRTNQQVKVFSEALKTSQCKTLTHLKLSHNKLNSLKIQQLTKSFPQHLQELDLMNNGLDSKACQLLSKYIQKSAIKKVNLENNRIGDLGSNALCLAIQDHDYLLYLNLSKNNLTEHCTIELSNYIKKTQVLFELYLHFNSINSAGGVNIWKAFYKNSSVKVFDISYNRTASLECAQWMAKVIIKQYPELMHIDISYNNFNEEQSIEIKKALDQNQNIYGFHYQGNCPKYSVDSTGHLRDRIQEAIDIQRKIEECKKNPQTTLKLVDDNEIQSLEIEDPHNHHNYHQQKISNLQSDAELHRNFRFRRIQGMKPIRQNYDKDSELDSCWICDGWQEVKFTWTPGKSGGMNNDPIFVHLNFESYKPILMTLHNGEYSAYRMCPPNYKVTYFFSNPVLGIQTTAKNQMITQTPSDDPLYQTKQSFLYNGDILIEGNRMGFVNEMFTEDKQSIMDRYFAKIFSKPREEEKTFDLTKFLTKREKQFWSYELSIFKNYQPDNEEIIDECFEYDYGSSKINKIIKDPLEYNEVKEVMREYYPYIFASYKFYASTLIGATIPCISSNAFFDLINSTSVISDKFRTGDVDLNFISTSNVKDINYPNVYEKALVRYQLMELLVRIAVDKYIRTQICKSVKESLRKLFEEDGVKLKLLEIDRSQDWRDMRYWNEQCDTLLKDRLPMLKMLFKFTSKLNPKQKYYKHLWLQFKDFRDLINKCDLYCDIFVERDAYLVFLLSMQTSVDELFLLKHFQMEFYEFLEALARCAEKLSLIRTQDQLGIDERRQQPLFKKLDALTYLIYVRLGEFIKSYFKDSEDLSDFDKCMQKQYGQVIKQPNPDEEEEVEKLTSQQEEKILEEQLKTIVVPPQITVVQQNKKTTGMTFLQVIKQAQQQKQLKNLFNLTNKNNQNDYIFYNNVDRIFQIVFNDTEFFNLILCSFRIQINQGITLFKFQSTITKNNRIXDVDLNFISTSNVKDINYPNVYEKALVRYQLMELLVRIAVDKYIRTQICKSVKESLRKLFEEDGVKLKLLEIDRSQDWRDMRYWNEQCDTLLKDRLPMLKMLFKFTSKLNPKQKYYKHLWLQFKDFRDLINKCDLYCDIFVERDAYLVFLLSMQTSVDELFLLKHFQMEFYEFLEALARCAEKLSLIRTQDQLGIDERRQQPLFKKLDALTYLIYVRLGEFIKSYFKDSEDLSDFDKCMQKQYGQVIKQPNPDEEEEVEKLTSQQEEKILEEQLKTIVVPPQITVVQQNKKTTGMTFLQVIKQAQQQKQLKNLFNLTNVVQYFRLKEEEQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TQR7</accession>
    <name>A0A8S1TQR7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPENT_87.1.T0240130</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="431720at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008331">
        <property type="term" value="F:high voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000030">
        <property type="entry name" value="sodium leak channel non-selective protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000525">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000113">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000117">
        <property type="entry name" value="Voltage-gated Ca2+ channel, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050599">
        <property type="entry name" value="VDCC_alpha-1_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628:SF7">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="3" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="168"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="221"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="317"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="343"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="457"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="491"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="522"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="562"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="594"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="661"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="862"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="894"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="928"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="981"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1094"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1178"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1207"/>
            <end position="1226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1262"/>
            <end position="1281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1293"/>
            <end position="1318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1400"/>
            <end position="1427"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="15">
        <location>
            <begin position="102"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="15">
        <location>
            <begin position="458"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="15">
        <location>
            <begin position="858"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="15">
        <location>
            <begin position="1176"/>
            <end position="1434"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="378"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8153289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1601" mass="187192" checksum="88AC39F869B28CD2" modified="2022-10-12" version="1">MSSTYKKLGIEFKPFTKDQISDSIAQIQSMRLEQILVGESEQFDWNNLLQKPELVDCVNLEQIQPDLDENKQVGQFSAKKTLFLFKIDDPFRRSVTSFLSNKALSNFIIVITILNIVAYSIPQSKISIGIEVVCNIFFGIEIILRVISQGLLFEENTYLKDKWNCIHFISFIFGWLILIDIDHIVLTVFKAIRLLRVIRLSEEISPLKIQLEAYISSFSKLGIVIIPFIFFLLYFSVIGLHFFMGLTTMRCRITPEPEDGIWEASEEVPYLCGIYECPEHLTCGSPYQYGLEWDHKENDSEEFMWNFLRFDNLPNSLLVIFTYFCLIGWSETNYMFWKAMTTYFTAGFFLLVLVFLAFIFSNLLLATFYESFIVKSSIKNSHQKVKKQQQQIETEINQKKQQQMTDRLALMSKKRKSMKQEQIQNANFTLFFQDHQQIGFKKGISLTNISNELSQSIYFAFINNLIIILCTLSIVIDYDGIDETMLIKLQIIEFVCTLYFYFELLIFFFAKGIKNYFKKPVNYLDFIAIISQLILFCILYILDENVIINQNRIIQVFKAIKLIRIIKFSYSAKIFYHISILTRAAIQTIAKLKSIISLWIIFAIFIALVGNALLKQQLSDHETPIHYNDFSNSLMAAFSIFYNEEWHLTMYRYGRVTPISFIYHIISILLGQVIFIKLLSAVFLNEFTKQVKIQEQNVKPIEYKQAAIDLMTTFKQFTSSSQLRSTHQLTRGISNFPGNKSLIQNVRQGILRNLKLNKKITKIIVPQKSQLELDNLDFLKDQQLNTNKPQFNENENDELLHQPQAILQRSRDLSIQVDQNIVTSQQQIQTKVQVMKTLYVFNVDNQFRLHVQMIINSRIFKILGFIAVGLSAVRTMLVTPLLDPHSQLYLSLDILYIIITSLYCLFIFLHIIAHGLYGNQNAFLTQSFYNVLNFVITVIEIVVVTTSIRNHVINFISSLRVFEFIKLGAEINHSIKYAQIALINALVKMVQLSIFCFILFLVYGTFAMKLLKGYNYYCTEVDQEEYQVHSNIDCMDYGGSWQKHRLGFDNILDSALTLFVIVTSEGWSPIMEQIWSIRGENQTPEENYNRYWAIYFQVFFFIGNTCMLNMFIGLVVSTYDESKLKAEGTYELEGNQREWCDIKKSIHKLQPKVKSRKPKTFIRKIAFFLVKNQFLKKIYLVVIFINTLSLSLCYVNQGIQYKNSLEIINLICILIFIFEIVSRFIAKEFKIYFKELINIIEIFGIWICLAEKIIDLNGIGEFYFLRCFKAFSVSIQMLRNFRIIKRFNRIERLFHSIFSVIPNALSMLYIMFVFLFIYTSLGIDMFCYLRPQKYIDDFDVHFRKFTTAMFSLVRVASSELWWELLVDALHTRSPDFACIEIDDYQDFLVYGYNGCGTNIAYVYFITFHLIFSLVLLNLFIASILGAYEEHAKQEQNAISKYQLNDVLSYWVEYDPYGSGFLNYKQFWRLSSQIAICFGVSGKDLLNPANKKNFLKALNIPLYEEKNGLMGYLFHDVIVSLTKISVELKYGVKDLEKKNQQENEFNHDYYRQFVKTPYNSGQMTAIIYLQNKARTLINKKRGINFLNIEALRQELQQQAENQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TR85</accession>
    <name>A0A8S1TR85_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0260013</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8154528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TR85"/>
    <dbReference type="OrthoDB" id="289098at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050498">
        <property type="entry name" value="Ycf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE SPINDLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="329"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="627"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="37"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="998"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="834"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="58"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="998"/>
            <end position="1011"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8154528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1073" mass="124677" checksum="5EDED23E0C7D3EEC" modified="2022-10-12" version="1">MLVRSSLSPRRKCFTPSMWEGRQQQTIRRSQNRYLKTEDVDHRNVQSRNDDSKRKSGSIVNNKKNKSMTNHKASLNSSFNTKNLKTIQLSTRKSSLQNKLHPNSTTSEHHVHQYFSQSPAKRILNCEKLIKAQKFSEALKDLEEIDQPHINPEYIKEIAYFKGLIHMNCRQYDQAINQFSRAKQSGDLNGNTVILQALCLKKIGNYSDAIVVLKEFLSKKYSKLQPTYYDALIHKGKLLMKIKKLQLALQDFNQASVLENDQISFQGTLGRADCLRLSGKIAEALNVYETIPENEIILMRKIYCYIELQSLDRAMEAINLILINDPNSSEALFMKGQIYINKGQLNEAILSFEQSIKQNNSRKAVTKSLQEIAKIKIDQKDFYSAYYTLQREDHLEVDKESIMKLRQFTEGVIFLMKRKYQDGVNVFTQLINNYQLGEFIKKIIFLYRAYGFICLNKFQKALNDLIYVQNFQELDQPSLYNKIICEGIVMSQSSQFEKAQSQFQKASKLFPGKMEPHFYKSLTLIQFINKHMPKDKDKYIKNALKHLDKAVTLNDQNSNLLYHRGILRFYFGQIDLALSDLTKAVEKNEDRVAKYVYARGLVRACLDNPQQALNDFTIVINWDSKFAEAYLNRAKVFTLLGDRTAAFNDLQTYISLKPKDPDIHLWAGNLLFLIGAYEHAIKTYSHSPDIQNNVQLLRYRALCYIIQKELNYAMSDLNRIIELTGDKKSYIDKECLLALKTSTVGPEESQSMMILKLSQGSNKQVNFRLASQMLKKLIVLGSDGHVFLTSDLIFYRGIMKFYLGNYKKAMDLWTKSYGLKQQIKEFSQQSNNSLISQQEQQERLISELDELEFEDRTYNMYEYYYNVAIATILNKQKRKGKELLNQLADQLQESFETTIRDFLAALDSTQTKSATIFPYSNRLCCIFPTFKIGNLETRLSFCLPRITPPSMNPEFDNKLIEGIQSTDVDNKPEAPWIRRNPDGVIFTENVQQVELDLRSESQKSQIDHNQEEENDDDNSEQHQISINDDKITIEQRHQDDSTTYQTNYKKDIELLKQQLRLDRIVEQRLAKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TTB1</accession>
    <name>A0A8S1TTB1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0270149</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TTB1"/>
    <dbReference type="OrthoDB" id="310033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="318"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="352"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="386"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8155530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="468" mass="54823" checksum="E0BAC1BD7BD22957" modified="2022-10-12" version="1">MKQQKLFICQHIEHEADDIQGFCLNFECQDSKPQFCLQCCVDPMKHINCKKDLKGFAYISSFIMKFNQNIIDLKTQLDKSFAQVKKKYEEFSKQLEKMKLNFFEISQCLSQQDYQQMKDNLQVIKEGYQYFKNQEEIMKQNQIGTQLINIKKMIEAWNFDTEQQQIQGINDQCTLQQGLQLLNQHKWQQAYELLQKNIKYLEKKLSLATLFQSISLIEMNEIGKGIITRDQAKKINCNLYKDLLDFSNLELNKNSQNTFIIIAKSYALNDDKKFQQAIELCEQILKGQPQNLHALYRKQYEQAISCIDLALKNNPNYSIGYSIKGICLRSLKKYEDAIICYDKAIQIDPNDAFSYNNKGDSLANLKKYEDAIICYDKAIQINPNYAFAYNNKGICLRSLKKYEDTIICYDKAIQIDPNYVNAYINKGDSLTILKKNKLAIENYEMAIKYCSTDEKEFLNRIQQIKNSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TUG2</accession>
    <name>A0A8S1TUG2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0280035</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8155910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TUG2"/>
    <dbReference type="OrthoDB" id="290074at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8155910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="288" mass="34353" checksum="D021DF5EA569F194" modified="2022-10-12" version="1">MSKEQNKFKHILLVPNIVSDQAFLQRHKTFQTYVKKAHSDLYYLQLKKIGGLRKSIAEKADYGQVLQQIKSNRLQNDYMNQQKKNQGFMYNNQLWSDKYYNQIYQMTRLSQLSEVLSKQKTKGRDQLIKDFEIYFNKHVHPINDEFTEVQKVLPISTCTTKCSSRINSQKFLLATERRLNSKQDTQDQDINKIQSLQTSFHKSVNNKILNLLDEANNLHNQNLETIKKTRLFQTEQSSSRKIMYSQIQRRNEHINIQISMRNKQIHFKNKFVQERFQKEIEKLSAQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U0Q1</accession>
    <name>A0A8S1U0Q1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0330165</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U0Q1"/>
    <dbReference type="OrthoDB" id="193300at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051493">
        <property type="term" value="P:regulation of cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000059">
        <property type="entry name" value="RIKEN cDNA 6430531B16 gene"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010441">
        <property type="entry name" value="CH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052111">
        <property type="entry name" value="Spermatogenesis_Ciliary_MAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12509:SF9">
        <property type="entry name" value="SPERM FLAGELLAR PROTEIN 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12509">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06294">
        <property type="entry name" value="CH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="2">
        <location>
            <begin position="11"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="136"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8159471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="192" mass="22291" checksum="B60D51597FE8B911" modified="2022-10-12" version="1">MKNRDNLQQIDDELHELYTWVDTVPFSRPKKQIAKDFEDGILMAEVIHHFLPKKVDLQNYSLANTYQSKLQNWNTLNTKVFQNLGFQLTQNDIEQIIQAKNGAIENVLMILKSKIMNYQDNETVEPQKSSATNISINNNQSTIQDLEQKIADQQDIIAILELKINKLQQLVKLKDNKIQAFSSKIQQLGFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U5N1</accession>
    <name>A0A8S1U5N1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0330094</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U5N1"/>
    <dbReference type="OrthoDB" id="10391155at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8159363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="93" mass="10907" checksum="512366CD59554DB0" modified="2022-10-12" version="1">MICLSERKISIHQNKGLVQLPEISKSSELLIEQQSIVIYKNQQENKTLISRQGYLLYKITLNKLEGLGQNRQLFQIIQIASINIKNTLWRYFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U6E4</accession>
    <name>A0A8S1U6E4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0330261</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U6E4"/>
    <dbReference type="OrthoDB" id="285889at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8159663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="139" mass="16414" checksum="E45DD519F6793E81" modified="2022-10-12" version="1">MIDSDTITLDQIYGCNFKRVPLLYAEMEGTNPFQINEADEDLDSDKQIPDQPKQISPTQQKAQLKPLIKTRKRQQTVHKSPVNLSQFEKHIGFRNSFYKNNNKINLASRQRVEEVKNQEIGMFTKYVQRFQLQKLQIWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U6F2</accession>
    <name>A0A8S1U6F2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">cystathionine beta-synthase</fullName>
            <ecNumber evidence="4">4.2.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0340219</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-homocysteine + L-serine = L,L-cystathionine + H2O</text>
            <dbReference type="Rhea" id="RHEA:10112"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:58161"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="4.2.1.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.22" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8160204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U6F2"/>
    <dbReference type="OrthoDB" id="10259545at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004122">
        <property type="term" value="F:cystathionine beta-synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006534">
        <property type="term" value="P:cysteine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009069">
        <property type="term" value="P:serine family amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000003">
        <property type="entry name" value="Cystathionine beta-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000118">
        <property type="entry name" value="Related to CYS4-cystathionine beta-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="7">
        <location>
            <begin position="44"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8160204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="491" mass="54816" checksum="D1EAB05ADF1615C9" modified="2022-10-12" version="1">MIQDTSKDTIQCTYKRGLVEQQAPTPHSNFKFVPFKKIYNDALDAIGHTPMIRLNKIPKEYGLKCEVLVKCEFVNVAGSIKDRIGVRMIVDAEKQGRLGPGKSIVEATSGNTGVGLALACAVKGYPLYITMPEKMSQEKQDVLTGLGAKVIRTPTEAAWYEPESLIQVAKRMAAENPDIILLDQYSNPSNPLAHYEGTAEEILWACDDKLDAIVMSTGTGGTVTGVGRKIHERVPGCQVIAVDPYGSDLAMPPEINKTDIKSYKVEGIGYDFIPKALDRTEVDCWIKSNDKDSLTMARKLMAEEGLLCGGSSGANVWGALEWAKSQNFTEQQRIVIILPDNIRNYMTKHLNKTWMIENRLISYDELKESDHPLAGKPLSELNLPEIPLLNVETTTIGECLDYLRTYPAVPLQKDGKLLSVVFQKKLLVGIIDKKLNNRDLAKKVQSKDFVVVPNRLDQNQLERIVERHEVVFIEDGEKLRYATPLELLQIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U6L3</accession>
    <name>A0A8S1U6L3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0330296</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U6L3"/>
    <dbReference type="OrthoDB" id="299007at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050167">
        <property type="entry name" value="Ser_Thr_protein_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23257:SF964">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_50, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23257">
        <property type="entry name" value="SERINE-THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8159733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="771" mass="90393" checksum="FF1839584696681E" modified="2022-10-12" version="1">MIKSNGLVKIIDFGLFGIKKYQSLLHFYTNKSGFTAPELIQQRGLVVSGSQEGDIYSVGMIMYEMFEKKIPFEGLQLQNIQECLNKAVRPKIESKSLMDDIIRWCWQQEIEKRPLVDYGTIVVGSNIYETEFLDEIYIYYGNKTTNTISFKDGLIISPKKLELPEYKINRSERGDIIMAIIKEAMNLLEYSKLSDLGIINMFELQYVNQLKSYPSLRFQLMDREQPSILMDVRKSYFTMHNCYQIIKSLNNTDPSTYFKNKYVLLRYPEKKMIVQIKSVVFDQEFTYKGLCLEDYFKQKYQLRSKSNVYLETYNTQNIKKQFLIAEFCELKIDYIEQDVTIYCQQLEYWNKIINNSTSFIQFLNQYKFIIRNEQVEFKQQYLEPGNLVLNNSTKSIYQIVASRTSSEFEYYYSDFFLKNPTTFTTSMTINKLMILVTREIIELRDNFKQFLEKFDAFIKSRQYKISQPQIYDINNNLNEIIEKYVTEDAYVLFVGDSNTDFVQARITLLQKAIPNQIIILPIQDQEINRLLAMMTANLGSVPWSIKEINGLINNKRSAVLGIWKTDNSFSACLSINKYLNKYISSFGDLDQILTLLLATYYATHKILANQVIVFAEDDYTQIMEQRIQGVIQLIKDQGLAQQILKPEIIMVQVKDAIAERYFTYFERNFESYYCKNPQVGCIIPINEKQGRFSFHSQRGEQGCLQAQQLNISSANTKEIAQLYYHLIFLNFDLSSTTYLPAPLHYAKKLSKNQLQSEQYKKAIEKGFLLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U888</accession>
    <name>A0A8S1U888_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0350272</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U888"/>
    <dbReference type="OrthoDB" id="301427at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="95"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="29"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="95"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8160988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="291" mass="34218" checksum="4C5650D937C11ED1" modified="2022-10-12" version="1">MSDDDDVEIIHSGQSFESSPKQVFNYQIIPNLQSDNQKLLQSNKSLEIEIKQYKQLLEQTLETLRQSERDQNTLKLQLSQKDFQLQVIQANVEDRQKSQRIDSHKKIKDSNSNRDSFGNKQTNVSSVGGFLSSTQSPRCQCDSFHNRGLTLIETLKKIQLSFKQNEQAIYQQDLMKILQEFEENFKQFLTLHDQYAIEIHRNKLNQTEKQNQIKQPSTHSISDHFHSLEDQNTNQNPNEQIFNNDDLDTSQFESEQHIKLQFECFEISLAKVNEEEKQQWKQKLLQLINTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U8N5</accession>
    <name>A0A8S1U8N5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Importin subunit beta-1/Transportin-1-like TPR repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0350160</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U8N5"/>
    <dbReference type="OrthoDB" id="296539at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058584">
        <property type="entry name" value="IMB1_TNPO1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25574">
        <property type="entry name" value="TPR_IMB1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin subunit beta-1/Transportin-1-like TPR repeats" evidence="6">
        <location>
            <begin position="199"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Importin subunit beta-1/Transportin-1-like TPR repeats" evidence="6">
        <location>
            <begin position="587"/>
            <end position="719"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8160764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1131" mass="128196" checksum="F7FC275D62D1D6B4" modified="2022-10-12" version="1">MKMLTEVDRKDWENTFDDNCLQLNDLSSVAEDCLGKMVRDLGVKYLLPIFVPLIMQALRSPVINEQHAGLIAMATLSDKAAEHFQNELPSIMELILPLSQSQNKLIIYDLLTCLASLCQEFTPKIQINYGSQILQLIVTCMQQKISQKIQYISIACLVDFTRELVEDKEAAKNVLTPVSTFLIEQLYSVIQTNLTGSIDQQQQQILEQALSAFSALATSLQEHFTQYYDQMMPYMMQMMQTVTVNEVKSLLLECIGCFLVSISTTRKEQCKTDSNQLVTHFIQQQNKMESDDPAHTSIFFFYTQVATALRCEFGQYLDAIFPLVERAMRLDVGFSINSQAEGKNITKVKLDLKFLGMKSLSLNTSALEQKVEGAHTLVNLAEQCGKSFYPYITKTIVLMKEFINYKHSSQIQKSMAKCSEYLIAACTLETDMAQVLIQVTPMLITEFTVQSTEQHAGLIAMATLSDKAAEHFQNELPSIMELILPLSQSQNKLIIYDLLTCLASLCQEFTPKIQINYGSQILQLIVTCMQQKISQKIQYISIACLVDFTRELVEDKEAAKNVLTPVSTFLIEQLYSVIQTNLTGSIDQQQQQILEQALSAFSALATSLQEHFTQYYDQMMPYMMQMMQTVTVNEVKSLLLECIGCFLVSISTTRKEQCKTDSNQLVTHFIQQQNKMESDDPAHTSIFFFYTQVATALRCEFGQYLDAIFPLVERAMRLDVGFSINSQAEGKNITKVKLDLKFLGMKSLSLNTSALEQKVEGAHTLVNLAEQCGKSFYPYITKTIVLMKEFINYKHSSQIQKSMAKCSEYLIAACTLETDMAQVLIQVTPMLITEFTVFLNSKSYDRVAEIAEVLANCLNQVKAPILDATIIDSMFQTCNKALAAIVKTKESITYEDEEQFEADCEEIDLLLDHITNIATELISNHKCESVQTMMPTYYSFLNTKSTKSQTINSIAFFNIVLPLCNESVFQQAQNEILKCYSTFSKTADLEMKQNLLFGYGNIAKRLPNLDTTQIIELAQTIIEQPDCKHVDKVASYETALTTLGKVAMYCNIAQKEQLLTKFLDSLPHEYQDNQETHLMFIKEVQNNNPTLMQFKDSVIQTLNRIKTQDCNNPENELLCDEGRKLIQQLLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UBR2</accession>
    <name>A0A8S1UBR2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fumarylacetoacetase-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0370295</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8162288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UBR2"/>
    <dbReference type="OrthoDB" id="430630at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018773">
        <property type="term" value="F:acetylpyruvate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820">
        <property type="entry name" value="ACYLPYRUVASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820:SF7">
        <property type="entry name" value="ACYLPYRUVASE FAHD1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarylacetoacetase-like C-terminal" evidence="1">
        <location>
            <begin position="15"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8162288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="224" mass="25507" checksum="53FA0E899C55216B" modified="2022-10-12" version="1">MNLLFRQVVNPNNPKIVAIAKNYVKHVKEMGGDEPPKEPVIFQKPFTSLLYLPDGNGILQLPKHNHEIHHEIELGFMVAKNGKNIQKDKWQEYIGGYFLALDLTDREMQAHFKKQGFPWDLAKGQDNFFPITDLITKEKVKDPNNLQLELTINQKVVQNDSTSSMYYKIPDLLAYISQFMTLRPGDLVLTGTPHGVGPIRVKDNLFGTLKQGNETLAQLNLIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UCC0</accession>
    <name>A0A8S1UCC0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0380294</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UCC0"/>
    <dbReference type="OrthoDB" id="282158at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035739619" evidence="1">
        <location>
            <begin position="22"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8162891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="107" mass="12188" checksum="D597A12029658ABC" modified="2022-10-12" version="1">MKSLLFGTLLFCLSLSQLTYEECKRDCTMDWLDCTSGCDSIGNPSFCHSNCQNMVHGCLDECPKIICIDECRILLEQCRKNGTGICEVHFNQCKLICEDTYNENQDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UES8</accession>
    <name>A0A8S1UES8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RanBP2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0380082</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UES8"/>
    <dbReference type="OrthoDB" id="430695at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034870">
        <property type="entry name" value="TET_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23238">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="8">
        <location>
            <begin position="17"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8162523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="162" mass="18816" checksum="DB4D7EA454774F0F" modified="2022-10-12" version="1">MAQQKEKNKIKINHKQRQGDWICSNCNNMNFAFRDSCNRCHNIKNIKHKENNGFKSALFLTESNGDIPPISDRSNKSSGERNNIGNNKFSFDKLPSIEPILKQMTKEICIIIYQNMKNQNMITLSLNGNVNNAYRLINTIKQIVFNVEFKVLESLQFDLQLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UF69</accession>
    <name>A0A8S1UF69_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SEC7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0380239</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="18431at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23022">
        <property type="entry name" value="zf-HIT_DDX59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1000.11:FF:000002">
        <property type="entry name" value="Cytohesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015403">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032691">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HUS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF375">
        <property type="entry name" value="LD29171P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09324">
        <property type="entry name" value="Sec7-like_HDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12783">
        <property type="entry name" value="Sec7-like_HUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="1">
        <location>
            <begin position="568"/>
            <end position="756"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8162787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1613" mass="189896" checksum="D8B4F48FC5847F42" modified="2022-10-12" version="1">MSLSLSDLINQSLDKIKKAITKKQFELKQTIDEVQQLKDYYDANHLIKVYQQCIESKQVKLMELALFDIKNLVEQGFFAGEQSIGDKRVIDVILDIVISCQQEKEETVQIHMIKAIQTIMTNRKHHIYGELTTKVFQLLINIHSVSKIVIIVNTSKEACQKIVSTYFQRLEDYGILTEDEFQQAIQKQENQGQLILGKCRAMVNAEQYMRSLMTSLIDEVQMYSERKQIYDRQIEDLNQMKVIDLNLQEPNLRNVTVEKNQIQVYVTNEQNIKNGKFGWCVVCRKQASQYCKETKVPICSKECKFIHLNQMNNFSQCYQHSNNSELYCKDAQEILEMLCQLTQRDNTNPQLNQIVIKCKILSLELIYEALAQSNIILQNKPKLIQVIKEQLLESLLKNSLSTEKQLLILTLNIFIQLIWKVRSHLKKELEALIENVYFKFLDSSNSSFDHKQYTLKVFNKIMTKPRVVIEIFVNYDCSLGQNNLLKKILDMQCRIIQGRFSKQEFQASITQNQETYLKSLCLDNYYGYIKCIKEFCEQNEDSYNIVQVQQLDDQEDTTIQSQQLSQDPIEKQKQMKLEMNKAVQKFNFKPEHCIKHLIACQFMETRDPKLFAQFLWENRDLNKDKLGELFGCSSEFNQQVFQQYIDFMNFKDLQVDEGLRYMLEFFTLPGESQQIDRIMEKFASKYCIDNPGIYQSAQAAYTLSYLLMMLQTDLHNEKNLDKMTIPQFFNLAKGINDGENLPQDLLLGFYQRIQKTPLALHAKEQAKRALEQANQVDQRKRHAMLAKEAEESLKKWFKEHPNQDAYFYANSIDHVKSLLQQTWSPIFASISVFLEQTEDQQQILLCFETIQSFIYLMGRFDLDEEKDTFISFLQRYCTGIPNTYRQILGVQALIKAAIQSGQYLRKSWRVALQLVSKLEILHQVVKKIKVDSPQKESYNQEDIQNIERLFQSISYDQIDKIFNMSINLDSNSILEFIRALCELSKEEIKQNRTFLLSRMIEVADFNMNRIKIIWSRMWEIMREHFLEVGCHKNVDLAIYAIDQLKQLSCKFLQQPELANYHFQKEFLMPFEQIFSHSQAQQQYKIQLREYLLSCMCMITNVCFNSLKSGWKIIMSIVNQALQEDQQQLIRLCVQITDKIMEDVNNQQVNQEIYMELIQALIKLTKNKDINIVENAIKQLKTLVDHIALIKKNDNKFLDSLWIPVLSSLSILYSDERVAVQQQSVQTLFELLQKHGVYQSNEFWKMILRGVIRPLFDEIQFSKLQYTKQQQSKKSIASTCKMTFQLFTDLVISQIQQMQPCINDLIDIYIQLVLQTQDHISILCLQSLKKIIQNVGQYLTEDNWNILIEQIQHLLQQCSPTELLEAFNLDEDFQKPLDELLKEEIRPKKFSFKINALECSSKQSIQERCLEILEVQVTQFQNQITQQNKQLILQLFQEQYQKCRKFNTNLYMRYFLEQWAVQWNFVKRSSQEFDDLDNTTQSKQLSFINIELLAAKVIIFLSNDPFSFIEQLIQRFLDAYNGFSQPQHRQDTLNGNLEQLRHLESQIVISRLQLLFMETVFPFLKQNLKSKVVSKWLIQLLKAGLNVSGVENKEYNKVLVNLLEEIINCENNVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UH71</accession>
    <name>A0A8S1UH71_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0370158</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8162016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UH71"/>
    <dbReference type="OrthoDB" id="10249433at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052920">
        <property type="entry name" value="DNA-binding_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43358">
        <property type="entry name" value="ALPHA/BETA-HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43358:SF4">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="69"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8162016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="425" mass="48947" checksum="4617BA28B4230387" modified="2022-10-12" version="1">MKYFQNGLNQICQQIIRPARAEYTIYDLSSFQIQEETQYIREDFDIINPRQEVIKVSQYVGQNKSDVCIIYLHTANGSRMEVSKYVSMIIKNGFGLISFDFTGSGMSDGDIVTYGHREVGDLQTVITHFQSSYKQIILWGRSMGSAIAIQYMQRFNNLIIKGMILDSPFVCLLDVILQMASSRTKIPNFILKSLSTFVSNELKKQAGFDLDDINCLKKISQIKCPAIFVTSKQDTIVPPEQTETLFKAYTGIKKIQYINQQHNGIREHSFIETLIHWFKKRTPIFERLGVGTQIKQRIMHKQKNTLGSIKQVSLDLYKERSSTLEHQRVINISISPRQKIMDQKKLTAEEKYNQLLETQRSLYIDRSASAQRIQISNSIDYQMSPMPLAPLSNKIITSSQHNYFSHSKPITFTKVPHKTKDGTHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UIN6</accession>
    <name>A0A8S1UIN6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0380106</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UIN6"/>
    <dbReference type="OrthoDB" id="287414at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032006">
        <property type="term" value="P:regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015019">
        <property type="entry name" value="LAMTOR3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01278">
        <property type="entry name" value="MAPKK1_Int"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8162546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="127" mass="14928" checksum="2DF27C7ADFB17DE3" modified="2022-10-12" version="1">MQNNPVLQRFLQNNPFIEGIVLMDKEGIEIIASFQNENSKLRTGAQSLMYVVAIQQCNENLRKLSDSETKQITLVYDEWILYFEIWTHSILIFYCNVKANIVSLKQLGQDLKRILFPYNDLLEKQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UJ53</accession>
    <name>A0A8S1UJ53_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zinc finger C3HC4 RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0420036</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UJ53"/>
    <dbReference type="OrthoDB" id="300249at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger C3HC4 RING-type" evidence="5">
        <location>
            <begin position="15"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="218"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8165088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="432" mass="51150" checksum="0B69BA3042C751D5" modified="2022-10-12" version="1">MNETQDSDTNFEVACSQCQTTPDNYVKLDCDHKFCLVCLAYNYLQSQQQNPESQDIMKVAICSKCQYETHLDPDTIEAIQYVIKEIIIPLIEQNQEEHNMDNDFDLKQSETIRESKANLATELINDEKFEQKKDKYANNLQFQKVDQTKETSEIKDIKKNKESLIGKIDMAPALNERVQLYLERLDKSFKNKFESIRDIQDQKFQFQQKCQSTRDEISQEFQKQIIALEQKKNSLINEVNALETQQLLEIQKQENEYESQIQLMAKYQEELRNLKGIPEDLSKFLIEIEKIMTAPSQSLQIKSSLLSSQRQLIRIQSLTQQIEERLTSDSKMITFQQKFSPVKNDRTNEIWNKINESDKKSRLKESSISQQRSNQKSRWKETILEIFDKKEQSFERCSNNKYNPLNRTYNIHQVLNMQQQSFQIQKENLLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UJQ2</accession>
    <name>A0A8S1UJQ2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zinc/iron permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0410157</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UJQ2"/>
    <dbReference type="OrthoDB" id="10263369at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF140">
        <property type="entry name" value="ZRT (ZRT), IRT- (IRT-) LIKE PROTEIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035834075" description="Zinc/iron permease" evidence="6">
        <location>
            <begin position="21"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="262"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="295"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8164685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="319" mass="35895" checksum="ADE8434939136B8C" modified="2022-10-12" version="1">MLILFLIILFIAILPYKSKTFRENKPLISISQAFSGGLFLSVALLHLLPESQEKYQQSQNIGQIKKKELFPFPFLITILSFALILFIEKIVTNHKHQHQDHVDVQPTQNIELLKSDESICCSQIGACCNQVESQAQEDLLRNAISSQVKMAQRVGFNEIKNKTKIKKPNKSSNLTPYLLQLAVGIHAIFEGLAIGIESNLSRCIGIALAVFCHKWAEGLTLGLAFKKAKVTYSKAKKLIFLQALMNPLGISIGWILSQNKIIIVSIFYAISAGTFLYISTIEVIVEEFNVARYKLFKFLAFLIAIGFISSIWLLEQIDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UMD9</accession>
    <name>A0A8S1UMD9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0430014</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8165564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UMD9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8165564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="273" mass="32285" checksum="8BAD2086EB965334" modified="2022-10-12" version="1">MFLVQQFRSQVPEELLVDYFDYQNLKFTKTKIQISLTTNKEIKYISSEGSILRIDYIKEPSKKYDLITNLEQIQHLKWQGEFGKNDKKIGRWIAIWKGQTLEAVNRWYSEDGLQSGLQTELINNYWSKADIYVYGEYYNYQKQGVWKFIIQDNMIVGGLYKYGQKVGKWIDLNDSVQNQNQIVYNGEYKNGNKIGLWNILYKSKDSNYKIIGGGQYDENGQKNGRWIDLNNVFWNKSEVIYHGQYKNGLKIGKWDTNDLQNTQNNQISYSIIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UN71</accession>
    <name>A0A8S1UN71_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0440169</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UN71"/>
    <dbReference type="OrthoDB" id="270720at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8166480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="321" mass="36917" checksum="A2EC8663330AED6C" modified="2022-10-12" version="1">MGICKSQPVTTEDLPTMDSSGHKQTEIVIKEDSTPNYDENKIVIIQALYRGHKVRKSLMTTLQSPETSAPTQVEQQKFCFDPNQLYDNDQSSILMKNGAIYQGEWKDGKANGKGKYQFQDRCYVEGTWASNELQGEAIYVNGTEMYKGQWLDNMFHGVGEYIYSDGRIYQGEWKKGLQHGMGKEIYKDKSSYEGKFKEGMKNGLGIIRLADGCVYEGEFENDQFHGYGTFIWPDRKKVFEGYWKNGTKHGNGTMKWGDGRIYSGQYLEGFKHGYGEMQYSDGRCYKGKWKYGLQDGMGIFVDKEGNEKKGFWLKGKLKKWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UP88</accession>
    <name>A0A8S1UP88_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0430006</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8165552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UP88"/>
    <dbReference type="OrthoDB" id="2687506at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="54"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8165552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="161" mass="18883" checksum="47B06FACC9A6FC5C" modified="2022-10-12" version="1">MLSKYQDQEQLFNWRCNLSGSEFYNVNISGRNLNGSQLFYCSRKNLKILELNQLVGHTYDFKQVCLSLDGSTLASSSYDYSICFWDIKTGKQMELLDNSKKIIQLILKQHFSRYLFLLSISQPLILQFSIIQITFNLIKKYLDLQSQFGICRAKDFRTFLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UPG2</accession>
    <name>A0A8S1UPG2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0430048</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8165632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UPG2"/>
    <dbReference type="OrthoDB" id="302660at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001131">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="16"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="304"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="419"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="462"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="304"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="322"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="342"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="422"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8165632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="549" mass="64386" checksum="EA0DE9D98A62D5E5" modified="2022-10-12" version="1">MNFDQIESEKLYNNRFFIKKKISAGSFGVVFLCEDLQTKEYVAMKVEKDDMNAIGLDREIQMLDELRKIPGIPKIIWAGNEHNHNCLVMQLLGRDLSYHMKDLRTFSLKCVVNIILQVIQIIEGVHRRGIIHRDMKPENIMTGKDQELNQIFLADFGIAKFYREPDGTHAPFKENKPFVGTTRYASINAHKGFELSRRDDLESIGYMFIYMLKGKLPWQTFHNVNEKEKVKIVGQMKSQISIEELCKECPPQFYQYFQYVKQLQYKSSPDYRYLYSLFEQIAIQNGFKHDKRFDWTEFSQGTTKISAEQTQQETRQQILRKNKLEDKSPKNSDININRKELNQQQKGTSQSRNRSRHQSEQCVNKSGNSVNSYSSSCLNSQQSSVIINYQNSQFTQSNVRIGSSKLLSRLQERGIIHKNSDSRGSIIQQQSSKSFRERNQSQQLIQQVISQQQSQHNNSQMMSRFQEDFQDLEELEERLQKDKQSIQRNQQHRNNKPLKVKEIQHHFDQNENPTDIGGMTQLEDDACLENKVKIYNFDAPKLKQQLAKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1URF7</accession>
    <name>A0A8S1URF7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0460267</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8167175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1URF7"/>
    <dbReference type="OrthoDB" id="306499at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="358"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="52"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="358"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="408"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8167175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="417" mass="50935" checksum="4CAF6B6BEB2F4E1E" modified="2022-10-12" version="1">MINRFEDKLALGLSRTLKDQENMMPKSSLMKDEQKIQQILTVKQKEIEYKMNQEKERFKQQYELEIKILKEDLAYQSHAAEMEKEKCVKLLKDEIQGLQDEIQNIKIQNIEEISNQKSKQQRELQQQICEFQREKSQLLERNVKEIKKMEQLRCQELEQWKNRTLMDKDQTLQLFEEQKHKEIKQLTDTFTYQLQQQKQENEQSLRLQKTQYDQKLQKISDQLDVERSNCEILRKRLQEQKKQFDLDNEALKKEKQIRVEQLQIEIFQEKQKYQQLQQEMDQMKFNLAQKEQKLIEEIEQKEVELRNQYHEDMNQLYQRQIEVAQSLELQYKELDEAAEKKYNKLKSQYEELQSIMRDTLDKQERKPLRPLQQQRRIGSKDRQQEEETGLQMLNLLEQAQRRSSQMSNRKELKKPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UTC8</accession>
    <name>A0A8S1UTC8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0430090</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8165716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UTC8"/>
    <dbReference type="OrthoDB" id="297076at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="293"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="48"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="145"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="240"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="300"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8165716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="348" mass="41260" checksum="6055E01DC6DBD58E" modified="2022-10-12" version="1">MNEIFKLVCFDGAQQKKWSQCKDLDSQLQFIRDAIPQKINEATKNYDYEALEKQVQKYEHEIRKHIRMEQQIKLYAESLQQKLEEQEGINECLNNTKQLVNSLKKENQQLIEQVKRLQSENYMLKTKLIDVNDRINSQQTSPNYDKTKNQDSYHIKNSQRYQTIDFVGPKQKLNQRNMDDYSKQYAEVKTQISNTIDSSILKMLQNRRDSLKLNDAYKSSRDSSTIEKNDSNKIKSCINQQRAASQQQKTQHKQMIDPSIQQVKTTTENVKFDLKNQSDNLRAFLNTQSFLCNKKSKTNSRPDNRYNKSHSEHQNANTNQQEIQKSNKIQKEYLQFSQNYQKYLDNQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UTY2</accession>
    <name>A0A8S1UTY2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Trimethylguanosine synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0490246</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UTY2"/>
    <dbReference type="OrthoDB" id="444258at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071164">
        <property type="term" value="F:RNA cap trimethylguanosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000724">
        <property type="entry name" value="PRIP-interacting protein PIPMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019012">
        <property type="entry name" value="RNA_cap_Gua-N2-MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14741">
        <property type="entry name" value="S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14741:SF32">
        <property type="entry name" value="TRIMETHYLGUANOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09445">
        <property type="entry name" value="Methyltransf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8168698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="257" mass="29626" checksum="F3B055A065ACF562" modified="2022-10-12" version="1">MIETQKVVHRKLSILGYPLNEFFSLDSLKLKITKKNFTCQLHPGISIDIPKQIQKFYRKRFFLFKKFNEGIQLDEESWYSVIPEEMSIHIANKLKGCSPDSDVIDGFCGSGGLAIQLAKCFKTVICIDIDPIKINNITNNLQVYESIATVIQMNFLEYTHTNQDFILIMCPPWGGLNYSHQPYDLNSMKPSLEDLLTKGLQMTSKIVLQLPKNIDIQQLVSIFKDVTDKLRLELKPIEVEMMLINEQINQLIIYYGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UUX2</accession>
    <name>A0A8S1UUX2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0470253</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000047">
        <property type="protein sequence ID" value="CAD8167702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UUX2"/>
    <dbReference type="OrthoDB" id="285841at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8167702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="495" mass="58622" checksum="97813297ADFD6913" modified="2022-10-12" version="1">MIINCSHQKIGYLIEQQMIKEVCDICNDIECIESHKFILKTDLLDFQQKALPLLTQLQTILYDQLPNQIDQLNKMINQAYQGFTKSSQKKQIENLMSILQNEEQKYQFQLINFYIARDQIKQIKEKLKEVIDFELIIQRCISQFNQNQNITKEVQTINFDDDQDLIQTTNKEQTDQLFIKQIDIPILNQNIIEKSNNFEIPTTFKFKRMYSITWLNENLLGIAAGDYNPYIIFDPQMQIIKQQIFQEKNGVYDSIAIGNDRIILAYVHNIKMFQWINNQYIANKLEFEESQGTPSKLYYCSQTQSIFATNTFFQVISQNYVYQWKLDSGKLIKYKIQGSTFGPMCLINQKLIFCFSLWEMKSRSQRQNLIYFWQYEQNTPIKKIDTSQPQYAMIVRMNQLFGFGSEITIWNLDNYHLIKKITYPDKKLNPWCVCSFEQSLILGCEDGNIMIVNEQFNKWDKIMKMRAKTMKMNVLKDKCVIAALDCSFSILQIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UWK9</accession>
    <name>A0A8S1UWK9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">1-phosphatidylinositol 4-kinase</fullName>
            <ecNumber evidence="2">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0500171</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19877"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.67"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8169121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UWK9"/>
    <dbReference type="OrthoDB" id="10264149at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000016">
        <property type="entry name" value="PIK1p Phosphatidylinositol 4-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF22">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="5">
        <location>
            <begin position="395"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8169121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="669" mass="79290" checksum="77345DD2CEADD28B" modified="2022-10-12" version="1">MNQNIQISIDESFRKIICKDVQEQTLMEFLERLNQLNYDETSTYLSGLVYLFTRKRWPQLGEYLLDKCAEHIAFYEIIKWSYEAYTFKERQSSENSVTVFDQKIEEAMVNRISENQYQEKESRSNYVNSIQRFVTCLTVLSLHLKNVPLNERQLYLRRNLIKGNRALESFRRNNPGIRYCKGIILPFKGRSQMVVRIIDTEAQSFNTKKRVPYKLVVETVDMDEYEQREQQIVTLQKQLSIDSQAFLEFDVMHQFDYTVFEDFLQENKDKLARQQIINQNKKRFDQQFPPLKKELIIRRRSCIDKIQSYTNKQLYEKDFKELFKQEDQLKHSDKELDNITFLDQIVNEYPNKKGTIEKIQKQLNDPKNIIQQQEIQQLQSPSQNDLKNGPFSEQWEDKRQVIKSLSPYGQFNSYCLRSLLIKGGDDLRQEQMMMQIIKVSEDILTQVGLYVKSYDIIITSVDSGILEFCNDTMSMDQLKKSMPSMTLKEIFNNIFGDNFEEAQLNFILSLTSYSLLQYLFQIKDRHNGNILIDNQGHIIHIDFGFILTIAPGGIKFESAPFKLTKEYIELLDGQESCMFQLFKSKLLLGYLELRKNVDKFAFILEAMKIDNELPCLEKFDMKTFKDRFKLELNEQQLEEHVHKLVSQSANNRYTKYYDDFQKLTNGIIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UX22</accession>
    <name>A0A8S1UX22_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0500213</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8169205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UX22"/>
    <dbReference type="OrthoDB" id="298073at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="111"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="414"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="687"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="753"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8169205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="818" mass="94922" checksum="AEC4FEC026B5D8B1" modified="2022-10-12" version="1">MLNQKCFMVNLLQSMILSYSIYHYFQNSCLLNVIQLIIITLITLALILLQNVFNKFMLQGTILCIELDMFCLIIMNIHNFGRIPYSQIIISQILIKDLFLDFKLGVKSELFAFQAIISIFLLVYSYLRIEFDREQPQLLTTLIISLYYNSSLPQQKQTQMTSIITEITNTNKQDILNSKRNVSQFDLDQNQNSVGIRDTSTTAENGIQETSIQCLELLQEGVILLVPESNTTNFPYSIKYINNATKLLFNKETEQEVLQYIDSLNTFQLFNGEPNEDLLFVSQQQRQPSLHLFRQATETKDYQQHRNLSLDVSQVQDRNQKFKIKQIIDTLLKSKNQESIVVQTQLNQRFTIFTNQQIQTSQTYINTENNQLLELTLTLSKSQCIIIICRDVTHRQKIRYLKEYDKQKSKMLSFVSHEYRSPLNCIIQMLECVLKQDQIINNSDINQQLQIALDNSNYILNLSNDLLDLAQIKNGKFKIEKVPFNLQILIEECQKMFELKAKLRGVQLSIQYNSTLPKTLFSDRNRIKQIIVNLLSNAFKFTQSGKITIILELFKQNTLRIGVKDEGIGISEEDQQKLFKAFSKVNSEESRKLNQQGVGLGLVISNQIAQNIGSTGLNIDSKNKKNNHYSYFYFDLFIDEILKKKKVSSFKVPEISLQSQEVDEIISFNKITSNLKEDLSTQQNCHHYLIVDDDCFNIFAFKRMLQELYKNNKHLFTNEFDIDSTLSGSESITKIKTKKCCSTCQGYKIIFMDIEMPIMNGQETTKFILQSFPNQIIIGCSGYTDQLEYQKCINSGMVDFLVKPIQELQLIQILKKYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V2P3</accession>
    <name>A0A8S1V2P3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cytochrome c domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0500121</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the cytochrome c family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8169026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V2P3"/>
    <dbReference type="OrthoDB" id="283834at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002327">
        <property type="entry name" value="Cyt_c_1A/1B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11961">
        <property type="entry name" value="CYTOCHROME C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00034">
        <property type="entry name" value="Cytochrom_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome c" evidence="7">
        <location>
            <begin position="77"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8169026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="236" mass="27288" checksum="29F0EBD190F1771E" modified="2022-10-12" version="1">MSEDPNEKWKKILLNPYGKSKDDVQNEIRAMHKEQQMIDEAITREALLQTIKAKKTYRNLTNPNTNADKSKLVPVDGDLKQGAKVFMRACASCHSLEIFTGKPYAADFSFQESSGPSLAQIYNKPAASQRVYEEYTMALLDSKIFWNSYNLYMWPKDPQAMCKGTKCLQRGELLESAEERADLVKFLKGFAKATSDLYRRSFTKYHGYEWQNYREQGLVKAREVAHQRQGYEPEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V3L7</accession>
    <name>A0A8S1V3L7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0550023</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lin-54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V3L7"/>
    <dbReference type="OrthoDB" id="6283463at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005172">
        <property type="entry name" value="CRC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028307">
        <property type="entry name" value="Lin-54_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446:SF34">
        <property type="entry name" value="PROTEIN LIN-54 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446">
        <property type="entry name" value="TESMIN/TSO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03638">
        <property type="entry name" value="TCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51634">
        <property type="entry name" value="CRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRC" evidence="4">
        <location>
            <begin position="58"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8171434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="159" mass="18044" checksum="2DB4333E17962D35" modified="2022-10-12" version="1">MRSFQVSKDSSFRILCPSNKIEMEEETVETNIQLQVKNIYRPIKIILPPPNLPKIITSTKSCNCKKSQCLKQYCDCFANGQVCSENCNCVGCFNNSLNNSQRKEAKLQIKARDPGAFKQAFKGCNCKKSGCQKKYCECFQNNLQCTHQCRCEGCVNCLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V545</accession>
    <name>A0A8S1V545_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0550165</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V545"/>
    <dbReference type="OrthoDB" id="25887at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061025">
        <property type="term" value="P:membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031468">
        <property type="term" value="P:nuclear membrane reassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012989">
        <property type="entry name" value="SEP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23333:SF20">
        <property type="entry name" value="NSFL1 COFACTOR P47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23333">
        <property type="entry name" value="UBX DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08059">
        <property type="entry name" value="SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00553">
        <property type="entry name" value="SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51399">
        <property type="entry name" value="SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEP" evidence="3">
        <location>
            <begin position="61"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="UBX" evidence="2">
        <location>
            <begin position="168"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8171717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="247" mass="27773" checksum="F593D2E41C04C561" modified="2022-10-12" version="1">MFKTLDDLKKNDKDEKKQKTSNSYAGGEKSGLSIENPDDYNELINKAKEGGAQPDGNDDPKEWCKITLWNNGFQINDGEFKDINDPENKKFIAELKQNQVPTALRSKYPKGLSVKLEDRKSEKYVPPPPPKYVEFSGSGVSLGQQQFVQQQQQVKVDLSKQGQIQIDPNQPTTNIMVRLSTGNTITLTVNTTTRVTVIQQHLLKMMNLPPQKQIQLISGFPPRPICNLNQTVEEADLCDSQITQTIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V6N2</accession>
    <name>A0A8S1V6N2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0570127</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V6N2"/>
    <dbReference type="OrthoDB" id="422005at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8172504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="179" mass="21194" checksum="30CBBBE33261B510" modified="2022-10-12" version="1">MIKFIARKIDRSTSFNKIEYKNEQEYQLEYPVNIQKHLIMSEQEYSDDYQLLKQNPPGVSLKLNIGTPKIETPSKLPYVLDENHQRQKGRMKFYDDAKKYGFLVLDEDGTDVFIHYDDLQAAGITMEKMKLYKIVHQNNPQYVKFTKNGGPYFEFTLMNYMGKYNKSRKAIDLQLLNQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V7X3</accession>
    <name>A0A8S1V7X3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0580036</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000058">
        <property type="protein sequence ID" value="CAD8172771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V7X3"/>
    <dbReference type="OrthoDB" id="547665at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="120"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8172771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="438" mass="51264" checksum="FF4B4EED63F87D76" modified="2022-10-12" version="1">MQQMLIPTSSGQTSLENNTNILYECIVERRHLFLNRKYYLQLTTQCLTLSEKQNQQNPKYIYQLKIDKVFTWSVLNNKIVGFQLPYQDKIKDFFGNPNDMVNLKTKMGALVCYQKIEMFYKKEEILQTGSFGKVTKEICRFTSNIVAIKSIKCQNNQSPAIKNEIEILKKLKQGNVLNILEIKEVYKDEFNYYIVTEYLPGSNLKQHLERKTKPYSIQEALQIMESLLKGLQSIHQNGIIHRDLKPANLMYHNNQIKIIDFGLACFNGKQLEQYPSCGTTGYSAPEVLNVWNKKQAYDYKVDLFSVGCILYKLLTLDGLFRSNNEKETFKNNKTCQFTIKEKGSVFDLVQLLVKAEPQQRLDCQQAIEAVQALLDYKYFDVNIWYTNKLKQYHIGRSQCELNQEPQTKLSRQCSLTNFKYQEPQTERQRNIPKKIDQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V8T9</accession>
    <name>A0A8S1V8T9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0580162</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000058">
        <property type="protein sequence ID" value="CAD8172995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V8T9"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000592">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF206">
        <property type="entry name" value="HSF-TYPE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00434">
        <property type="entry name" value="HSF_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="4">
        <location>
            <begin position="107"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="262"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="300"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8172995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="318" mass="37990" checksum="669BC178C94B226D" modified="2022-10-12" version="1">MILLYINFNELEEQQLPLYLKRQLSYKSTKSKRNFEFSFKNILNGYGIQYNSFSQLNSGKLHYQSIYQQIYFKLNNQNPEFEDIISFNSVGNAFIVKDQTVFSDFVLPKQFKHQNFSSFIRQLNMYSFKKIRNVNNQYQFSHHYFLKGREDLLYKIMRKNGVMQRCHYLKLQNKAKQEVTQLYSNYSELKTDIEQIQKELIYFYQQFDQFQSTISTLIDSSRQQIRELSIIKNQNSICNDLLFQVLANVSKSQPEQKYHFQYPFTNSTQQTQTTKSNGSDKDELNNDNENNNNSSKEDTNQSFQSNSFREPQQQLKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V8X3</accession>
    <name>A0A8S1V8X3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0600170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000060">
        <property type="protein sequence ID" value="CAD8173988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V8X3"/>
    <dbReference type="OrthoDB" id="289862at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8173988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="98" mass="12088" checksum="E29DE51597D6AC31" modified="2022-10-12" version="1">MNLNNVYNVVEEAMDKLLEYLHQNKYICQDYNQQNEFRNKFKQIVEDSKNYQSEQTEEDISELDFYLQNPINRKRHQSFRIFNPLVFLAKEIRQHEQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VAJ1</accession>
    <name>A0A8S1VAJ1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0590200</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000059">
        <property type="protein sequence ID" value="CAD8173585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VAJ1"/>
    <dbReference type="OrthoDB" id="297683at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8173585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="392" mass="46090" checksum="2C933560C94B5091" modified="2022-10-12" version="1">MSKKSTYVSIGREIEQARMTKSSGFNDVLNEKELRNRANSLLQKPQLKKFYKNKKDGGIIKPNKMAEQLRNTFIDKLNLNNLDSAAPPKSIVSHQSSTTTQPLCFEIDFDWLDYCDEQKFKQIFKEDLKDIPDIEQYVEDNQSYLCHIFAQCACAKCTCNKCICQYKKKLELNYAYGMLTSNKFDYVPKQNKCFTPIITQNHYDNIGLPVASMQFNSTQRDNYKWIPQDRPRTAAKSMYESHNLPTGQTTNNVRIFIQQSCSHLSIGRTILQIYLRLLNFVLLTNYRNQFIWKVKDENQPTADDIKQKQFHQTIQKIYPNQLRSIYQESFIQKNQKPIRVFLKPEEILQCNAYQGQYLTTKQMDYEGKQITVKDIQQKPWKGRLIKKLQNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VAR9</accession>
    <name>A0A8S1VAR9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribosomal protein L13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0590235</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000059">
        <property type="protein sequence ID" value="CAD8173655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VAR9"/>
    <dbReference type="OrthoDB" id="311781at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_uL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_uL13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545:SF41">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L13, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8173655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="357" mass="42541" checksum="06001A56CF1C7BC1" modified="2022-10-12" version="1">MQLFKRCLVARFSLAIPKFKHKEQPYVKDIPMPKRPKLDPKDPYEKYELVKPREYEFRQALDERHTKNFYPGTTYVAVHKGGITYHLFDASRFPYGKMCSKIAYYLSGKHKPTFRNNEPGKVTDKFIIVNGCNMYLTGKKINYKVLTYHTGYVGNLKQIKFRDLILQKPEQLVAWTVSKMLPKTVYRMDKLDHIHLFRGRFHTFDDVLPQILPHHTDFEYRGSILQDMKEGDDRTLVFTSKPVSQEQIKQDLGDIKQQIMDPSELDNDLIFTPFAERPQKIKLNMTQHEYDKLNRRRKRLMQRYRKYMPIPYRKTIEKADFTKSYVVKSEKQLNRLGLQKIKPLDDDPELEDETTKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VDC9</accession>
    <name>A0A8S1VDC9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0620016</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8174827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VDC9"/>
    <dbReference type="OrthoDB" id="431454at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10098">
        <property type="entry name" value="RAPSYN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10098:SF108">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8174827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="390" mass="45111" checksum="8D8E2CBE13995F66" modified="2022-10-12" version="1">MQQTNQRMSQYLIEIASLKYQSILLMLNSGGHTIDFEGILTQRQSEDKLLIVPEEVSAQIPPRVQFEVHTTQNKVKKLKTIKMAKLPTKTQFILELSENSDYRELLASIEDLERNELKKAKSRLLKIITNSKEGIKEADTKIEQKQSSVICIQAFYYLSKVQQSEGLIEEAVATQNMILQNFDIADLHIKGQLMLSLGNLYRILLKFQEAADKFYQALILYERLNWKVQQADCLLQLGIVYALINDYESAKLITYEALEIYRDNIIENNIKVGKAYYALGKIFYYSKAFEVATEYLLKSLKIHTDFYHNDYDFNFVQIYNLLGIVYQIQNQLEIAIDYYTLAVRCYRGTFDAQLGQILNNIGVAYLGLKKFELASDFFHNADQKTSPTQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VDY0</accession>
    <name>A0A8S1VDY0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0610219</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VDY0"/>
    <dbReference type="OrthoDB" id="304641at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8174665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="849" mass="101830" checksum="0DD25F884C0C6D9B" modified="2022-10-12" version="1">MLLTILKLDQQNKIFQEILLISDQVEIRKFSEEELNNIFNDFEKIKIGFECLCNQNIEKSLRVQFFIYLKHYLQQNYRTILINHKDDILNILLFYLNKVDQQIIPLLCKLINFMILNTPDSTQIIICIMDKLLLDISYIQLITEMMSNQIVKIKLKLSGYKYNFSKIYQFFNINDYNIIDLILNFYISLTNSLIYLLEDNDYITIINQYCSFISNNPQQSNILGKFSKIIIRLIQKKLPPEHIQQQLFSIITHNYLDNQYHLDRITAYRLLKILNQIQTSQKINYQKQRELLIFIVINIIYLDLHSYQYLYLNFDDENENDYYLLKIRNFGIDMLLTILKLDQQNKIFQEILLISDQVVPFHKNEESICIKKEAHYYIISNLFYKIPIHQIDQLFLQELLQCLKQTNNIYIPIKIQVLILLKRFLLKQIIQNQSQIDYLLSILKQQLQIIAQINNPNLLCAMIDLLVCLQLFYPQKNCLNKQIYLDLKLYYSLQNKIIFKQSIIKCMVELEQLHLGDNSIILIVQQELLKAQQEQNFQKYQLKMLIDVMSFIVEKQYTYQITLYLNIIYTLVHSSLQQLKHQQASLNLLNLFLKKLLIQQEIKSNDHEISIRMIQILFIDCLEEDPDLFENIVQTYYLILLHDVADLNIALQFITKYVKNLKNQNFLSLFCLFLFRLKKGLFQQEIQQLYKQLYEDIEQDDGRTSNELLCGLSILDINQYEIKEIMLNQQDVLYIMTNKAIQFINYKNKFYINYLINFYQSMIAKKHLIFDMEQLSLNNSFLVKLHEFITQLVIKQEDTQYSIINQIQKIFEIYYLTKLFISDQIIKKFQISIMIQYQDDLNLNELKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VE15</accession>
    <name>A0A8S1VE15_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0610239</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VE15"/>
    <dbReference type="OrthoDB" id="118550at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802:SF155">
        <property type="entry name" value="DEUBIQUITINASE MYSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802">
        <property type="entry name" value="SWI/SNF COMPLEX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="27"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8174705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="112" mass="13452" checksum="A9C044B6618B9086" modified="2022-10-12" version="1">MPLIDSEVTKYQCDAQDSSKKIQKKSGHWKQEEHESYLRFLQENANHNKGQRLFKRMSQVIGTRTPSQCRSHHQKFNPQKPCDKITQIKILKTKQLVRLYMNRHKEKDEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VEK0</accession>
    <name>A0A8S1VEK0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0620044</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8174883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VEK0"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002893">
        <property type="entry name" value="Rab_B04 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8174883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="217" mass="24636" checksum="314EAF9F623849C6" modified="2022-10-12" version="1">MMNSSADKTPLFKFIIIGESGVGKSCLLLRYTKDEFVSEYNVTIGVEFSSKTVEIDQNTKIKLQIWDTAGQESFRSIVRSFYRNVTAVFLVYNITKRETLEKLDGWLKEAKENASPNIVTVLVGAQNDLDDQRQVSYDEGKSFIDEKGINLFFETSAKSNQNVERVLTAKLVFLNYINETMRKNDNKISIALSESNANTQQQLQPQKKKKNDDSGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VGC6</accession>
    <name>A0A8S1VGC6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phospholipase</fullName>
            <ecNumber evidence="6">3.1.4.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0630044</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14445"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="3.1.4.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the phospholipase D family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.4" evidence="6"/>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VGC6"/>
    <dbReference type="OrthoDB" id="419078at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004630">
        <property type="term" value="F:phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09138">
        <property type="entry name" value="PLDc_vPLD1_2_yPLD_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09141">
        <property type="entry name" value="PLDc_vPLD1_2_yPLD_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016555">
        <property type="entry name" value="PLipase_D_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015679">
        <property type="entry name" value="PLipase_D_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896:SF76">
        <property type="entry name" value="PHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00614">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009376">
        <property type="entry name" value="Phospholipase_D_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="7">
        <location>
            <begin position="382"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="7">
        <location>
            <begin position="780"/>
            <end position="807"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR009376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8175415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="954" mass="112039" checksum="CD1324202A0B4E20" modified="2022-10-12" version="1">MNQQLLSEKLQLNGLKIESCGFIKTFEYLRELKLQDEIRIEDIGAHNIYKFRVRILNQSYIIRRRVKDFQQLESATNPDPLFVFKKKEVTKESMEDQPREKQQILFNFLKQFLQNEDLNALTQDVLKFFEISEVEYGDFRKYKECTLKKRAGGRFSESRCTRCGTLWGRWSKRYFYISNNGVMYCKGPFGQRAQMREQLVFDYNFRMKYGKAGTGYDRGIKLEFATRHLLLVAPDYFTFTEFLTALNQAEKECPYRQIHRFLSFAPIKESHCKWYIDGEGYFSDVMDALLNAKQYIYITDWWMSPDLYLKRPIAIDQNDQINQDSRLDRILQKLANRGVSIYILMYLEPTIALKHDSYHTKNFLERLSPNIMVLRHPSPIPQLWSHHEKIVVVDGTVGFMGGLDLCFGRMDTQQHLLSDLDVRRQFWPGIDYANNRMKDFENVHKSGESQINRSDPRMPWHDIAIKVSGQSVSDLVRHFEQYWNHVMISQNFPKRKQHQLQGNAKNPTNYYKIQDVYQQNSQDTIFQKYKNQIKEEYQSQYPNEESKEDQSKQNTQAYNPKQRLSAIFPNKQQCNKDFIEILEKIHGQNQEEEEEFEENIVPKNKQFRSSIMIQPKLQMAYEKGECQTQILRSASNWSIGCSSANTEFSIQIAYTHLISEAQNFIYIENQFFISAVNNNPIIENKVALALVQRIKKAALRKEKFKVIVFLPLLPGFAGEIDKDSAVLKVQLHWEYLTISRGGNSIIETLLKDSNISDPSQYIEFYSLRQHTKILDVPKTEQIYIHSKLMIIDDEYALIGSANINDRSLVGNRDSEIAIVIYDNKKKESIMGGERVGRSIFAQELRTSLYMEHFGLKYDEVIDPLSQQLQEKIRFNTRRNTMIYRQVFACYPDDNVKTLNDYQTFKSSGDLSKYDQLSSQIVGHAVEFPLEFLCKENLTIKILSKEIIVPEINFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VJV2</accession>
    <name>A0A8S1VJV2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0670003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000067">
        <property type="protein sequence ID" value="CAD8177061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VJV2"/>
    <dbReference type="OrthoDB" id="21330at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02410">
        <property type="entry name" value="RsfS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="171"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8177061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="292" mass="34514" checksum="25AEDA1824CF9D51" modified="2022-10-12" version="1">MFKYVNFRSIYSMARNVRIVNKLKKTQDVSQQFSMAYEDKNDEDDVIGSLNDIKLEQNTILKEAIKPDINLIKETVLFDGDDPYYEKVVDRPDKGTVWGLQGELVQQRDILFDPNEQLDIYKLISFLEINQIKDIVHYDLEDYGKQLPQRYGIVGTMFSSNHGWKVCKELVKGFMIQYQKEQEEIKNLNLRLGNEAMNAEPDQRGIMNLKRLHHLGIGTYGKPQISGQKDDEWLMVYYKQYMIHLLTEDSRQEVDIEAKWMYQPTEEHMDEYDKLMIATKTGNRRFFEPLDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VK72</accession>
    <name>A0A8S1VK72_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0640209</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8176179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VK72"/>
    <dbReference type="OrthoDB" id="285470at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:002297">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF98">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="5"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8176179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="476" mass="56820" checksum="8DB24741CAB33B7B" modified="2022-10-12" version="1">MQLKYEILQEIYSSQRVRIQKVRSLQDNQTYIQKTYLQNKNEGLNAIKMLAEIKNQFVLQVIEYLENEEQQLVIIQEYCDQGDLKTFTLQHIGYKMQESIIWSLFLQMAYALLQLNELGIQHRVLIPENILLLVRTEGYQIRITDFHKSSRQTLCQLPCAAIYLPYEYFFEGHYTQKSHIWQIGHILYFMVSQNLLFPSTTLENQVKEQLRRGRIQINIPDVYSQGLIKLINVCLTIRENQRPNILQILRMKEVRDALARPYYEFSLDQRRHLMHWINSSETAISNPIQYSFNEFDLHSPIQIQKDVVIKKNFKNTKLPEIFQQNQSIKKSSTDRILESDKIKTEIKRKEDLYLQQQLLSKTYTNRYIQGVVKPISFHKKIQLNPNIELPKFSIPYQKQLDPVFQNLLENKKQVCDQQIGETEYMFLRSQIKFNKTDKLLNYMQIRHPRVDSSFILQQINDIADLEEQRNHYISKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VLW5</accession>
    <name>A0A8S1VLW5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0690169</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VLW5"/>
    <dbReference type="OrthoDB" id="438641at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042826">
        <property type="term" value="F:histone deacetylase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052097">
        <property type="entry name" value="SET-MYND_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46165">
        <property type="entry name" value="SET AND MYND DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46165:SF2">
        <property type="entry name" value="SET AND MYND DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 6" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="179"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="7">
        <location>
            <begin position="292"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8178384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="641" mass="76161" checksum="00854675C580A4A4" modified="2022-10-12" version="1">MANIPAHVITIHSQKYDPPLQKIEKTPKLPISEMKLIDVEELMENKNMNNQYIIVQVYQIDWKNSNQYLIVRDFNEIWLNLTIMEQNKSCNYQINDWLIVKNPILMEFGIIIQDQLDVIAINEFTYQAMINSETDQTEPIQLKEKGNQYFKLQQYRLAYEVYSMVIIYDHINKTNLILEQCYNNRAQCLYQLGYIQESIDDLNQVLKLNPQNEKAIQRQALCYLKLQNPNDAKKLFESLPNNKTDKDIVFKLVECELMIKHQSGNIDLISILNQFVNQQQFYFENINNYRHKSIERKKSNLGGLGLFVNQPIPKGTVLIVEHPMHKIQENQTLGTHQRDNIYEEIRQKAINDKQFAERLFKLYDGSNNYAVKLIKQDFEYQLQNYIVDINRIENIFRYNAHAFQLLKVFTKNKQIKYLDQSEGLWFTLSQVNHSLTPNIFYYFLGELLIVVSAKNIEKDEEILVQYHPPLNQKEYQNNLKSHNIPMDQRLVQYNQQWKMDDNFNEIKTIIKTVKTSKNLTDLTTYTLSNPNIIQQIKEKYPIKYLKIVQQVCFDLFQVKRIKEYINLKFQGLVFQFDHYPQCSSIQDFFMFLTVVSQGQEIEKEIVNFIFKVGILMFGEAFLKNQNSEDLIFQLGFKQMKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VSR1</accession>
    <name>A0A8S1VSR1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CBF1-interacting co-repressor CIR N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0740032</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000074">
        <property type="protein sequence ID" value="CAD8180290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VSR1"/>
    <dbReference type="OrthoDB" id="2159131at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039875">
        <property type="entry name" value="LENG1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22093">
        <property type="entry name" value="LEUKOCYTE RECEPTOR CLUSTER LRC MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22093:SF0">
        <property type="entry name" value="LEUKOCYTE RECEPTOR CLUSTER MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8180290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="296" mass="35999" checksum="723F00D0913CE35F" modified="2022-10-12" version="1">MGGHGGLNILPQKKWNVYNMENRNIVERDKRKERERIQKEQRKEHKEQAGNRIQKLKQQRRAASHTPSRSVSESHSRSRSRSQSQKKQGSSKINNELREILEAKQHINLFKEEEQYLLQKENNQAKIELEKEFFKDKPFDLSTKFLGQYEQIKPWYIQQRQDQHQTQLQQKVKQQLQHFKEKQDNYYQILDLSKKYQEETLISQIETKKEEETIKREEQQDSAAIKEEKRRQKKLQKLGQQLQELLGSDDSELEKKRQKSKKNEREKLRKERLQREKVERSREQKLLSTIYEFKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VU17</accession>
    <name>A0A8S1VU17_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4-alpha-hydroxy-tetrahydropterin dehydratase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0730200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000073">
        <property type="protein sequence ID" value="CAD8180187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VU17"/>
    <dbReference type="OrthoDB" id="277398at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008124">
        <property type="term" value="F:4-alpha-hydroxytetrahydrobiopterin dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001533">
        <property type="entry name" value="Pterin_deHydtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599:SF0">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01329">
        <property type="entry name" value="Pterin_4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00030497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8180187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="173" mass="20119" checksum="DDDDAE6F2D46E72B" modified="2022-10-12" version="1">MIQLFRFCSGKITSSFPKLSFKVKDFSILKAEKPTALKIDDIKTLLKMHQLGATWKLQDDNSLYKEVKFSNFKEAFSFMTQVAIFSEQINHHPEWENLFNTIKIKLITDDVNNLTVKDIFLAYAIDNIAMNVQVKSAESTNDTRILEIAKIAEAWNFNFDQFHRMIETDSKQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VVB7</accession>
    <name>A0A8S1VVB7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0690081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VVB7"/>
    <dbReference type="OrthoDB" id="299625at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40515">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 157"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40515:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 157"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="51"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="121"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8178276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="407" mass="48860" checksum="16FD9C1A5C559519" modified="2022-10-12" version="1">MQLDLYKERQQQNENSQQVKAAEAFQSLVQYMNELTDIVNKIKLKNEAEFVIAYQNHMKKIKAELKELRNKTEEQHSIQTQNKINVTNNENELVYFREECLKLYQKVEMKSKENYELRYKLTELQKTNDFLEQQIKSLMKKVKLQEIDKEILPIVQLDQIFCTTTPVNQQKYNKRKLTEYQPKVELSSSLSKHHQSSIKEMFPVDELNKENFKYNDIVDKFTKYVTIIEQKYQNQNKILNERVQKLLISQNKKSIIRSDLEYCFLECVDAVRREIQKKRPLGNFQQYQSYLDTIQDFQQFYKEDKIKILELVVSNEKLLVFIYQKLFPNHVNLVLQSIKEDKNLKTFLDESFKHSVTMNNNNNESINVNHLMTQYKIEAPKTTREVRSISLNKQLEMRKGKLLFKQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VVH2</accession>
    <name>A0A8S1VVH2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0720220</name>
        <name evidence="2" type="ORF">PPENT_87.1.T0720222</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000072">
        <property type="protein sequence ID" value="CAD8179787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000072">
        <property type="protein sequence ID" value="CAD8179789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="323984at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8179787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8179789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="156" mass="17857" checksum="C2DEDB05D4C6C911" modified="2022-10-12" version="1" fragment="single">MNSQQIVVQLENKVQECLQGVQQQLDSSFKTIDDFLDELLLVPYPAVVPQNTVKFVPPVTQISLTSIETLTNQLIQEVKPLINLTIHKQIKNNQIQLQQQLIQTQNIQQNTIINNHHQLNIKPFTYNLLQNNSIQQNEYCFTIAINKDNSIVLAGX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VVZ7</accession>
    <name>A0A8S1VVZ7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0770167</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000077">
        <property type="protein sequence ID" value="CAD8181944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10324315at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="145"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1061"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1384"/>
            <end position="1406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1574"/>
            <end position="1598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1320"/>
            <end position="1342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1325"/>
            <end position="1340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8181944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1638" mass="196056" checksum="33BF8472062B219A" modified="2022-10-12" version="1">MMFYQIWMDLKESMNTSYYTSFIYLSIGAILNTSPLLEIYLKIADNDNYDQNQLYLILDSLNVSKYMLNSSILHSIYILIIFCFNTFIIILFMILYYLKKNNVQTNIAFTVYGQIIGYLIQPYFWSINGFLLQIQNTYWEQIQNLAFILLDFIAIVQQLLFLITLILFLHYDHSQKKNFLKINSPKTLIIQIITIFFNSFLELFINSSDQLNIAFPIVLVILYLLLFTQIHLDLPVFQNKQINNYFQIIICIQFALALNLLVSYFINFFEDRYCLQVILTFTLLIQLNSKIRQFLIFRSINNYSVTRIIDQRTIEYLKQHSEYLLLLRGIVQMHQSLCSNINCFYRSKFIYKISSKGNKKILINRDSIYDNISRISNFFLKCSYETMQQKNQDQSISIQLIELLCYKLKLYSVSLTQIDKLNEDKLNLFQQINIMNIKLIISRLIKQKNEQAYKSKLPFDHLLQCESYIKETVQIIINIFGYQLQFWNYLLNEEIRLIDELFILQKISRLIKKFQGLQKQVLNLHYKQKVPIRNRQWFNYFFFKLFILNKKLKLQDLEILPDFQLNLDQILKDEYIDEKSSDEEPINYYQQKFLISGNTFIIQINQSCIVKNCTNNTFQILNYNKQSLVNSSAVILMPQMFKQYHQQFINHFQQTGQSYNLYKRKKAFCIHDQGYIITVYKYLKYIYNYTSNQIEYIAMFRQIQTKQQYLLLNEDWEIDSCTQFLQDIIGIKSLSLFILAPKTIVFSEYEKFLTKENKEFFSLNAPNLDQNLSQLFYTQPSMIRGSFSSSNGQNSILQKQDEIKRSYQNFLNFDDVINIKDKQKSKIKQVLFNLRVPKQKYELVQEFQSKLSELYKQAIYTELFKRQLIVKNQFGKYQIVKSEFIRRCKIYQEIIKKEKFKIIKELFLKYTQSEESIDKILRIEGTLKLEKTLHSIKYILIKIVRTELIKENQKSYLSNCGVTLSFQTPLCQLISSQQTKRSTNTQLSPRHQYVQTYDQFDLYSNRESMGKVLLPEVQVNQDFGQNDQYKNINFITCNSDLLKEDTNKIEKYNPQIVQYEFISRLIFILQILIVFLSFFFTSYIQNQNTIVQSVNLVDYLYEYQIIVAESYNTQIDIELFNRNYTYQDLTKGDFIGFESDEQFYEYQQKVTQKCGDYMYFVFLEPKFLKMLYENFYSNSNESGLDIQTVYQVNVYILNMLSSQKFKEDFGISETFRQYMLPQFQQIQAHSMDYLFNQGLGFQEISNSNFILFLILQTILQISNLLYLIRFQFQIQKQYNVLFRVQAMIPYREKVAIIEKISTSINQFYYVTNNEYGDLEQFSPRNKSHHSRQTTLRRKSSSRYGNEKSILDIEKKHLEKSDNKSIIKFQQLSQQYSLKKIVIKIILNLLLITLISIAGVITIYYTLKNIELFNKNQFMFNTQNAWVLPAIKEIFTSQKQVDYNFNDTNAQIILQFYFEQISSEYPVFYESNIDEILYLFDNNICNRDNDIQLEQCELLINGALTRGIREYDFLLSKLVQKVLNPNDERYENITYSTIYEFNKLYYFVFDHYTFAKEIWVKISIETLEKHDQVSLILLITDIIGICLYFIIFNEFGYVLNIKREYKFIKMFYKSFMPNVSINQQKRLRVELIKARFIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VWZ4</accession>
    <name>A0A8S1VWZ4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0750242</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000075">
        <property type="protein sequence ID" value="CAD8181177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VWZ4"/>
    <dbReference type="OrthoDB" id="298777at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8181177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="488" mass="58122" checksum="F85290EE48ABE377" modified="2022-10-12" version="1">MNINQDNQILYYQDGEAIRMQEEFRPNQTQQDVLKNLEQLKFLFQHGKYGQYGKKTGYWYVSWNGSVLNSIGGYYEEGQKHGRWNQLFKNYHNSNKIYEIGEYCYNRKIGYWKYMLNDNLIGGEQYDDGGHEFQVGKWIEFDDGYNQQKQVMYDGEYINGKKVDIQNKIYGGVRAYDLGVRNGYFIELIDGFKFQSQVIYYGVYQNWKRAGRWFIQYKDISMYGNYEKMQLQIIIKYHKFSGGGLYDQEGQGLKMGNWIDFSDEYKWNSQVIYVGGYIHGKKVGRWEIQFRKSDEREFKKIGVGKYDEVGQEEKIGQWINIDGNYSDDSQVTYHGEYKNGKKVGRWDFWFNYNDLERGRKHIICKFLQLTQNGEYQQGKKVGRWDISYKEETDNWGIFYMNLDFQNIVFKNNRIKKENKLIGGGQYDKQNQGFKVGLWIDLGDQFKKYEQVLCKGEYINGKKVGVCKVLKKDQNENDEEFKLEKSNLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W011</accession>
    <name>A0A8S1W011_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CD36 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0780031</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CD36 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W011"/>
    <dbReference type="OrthoDB" id="284331at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005044">
        <property type="term" value="F:scavenger receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002159">
        <property type="entry name" value="CD36_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11923:SF51">
        <property type="entry name" value="LYSOSOME MEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11923">
        <property type="entry name" value="SCAVENGER RECEPTOR CLASS B TYPE-1 SR-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01130">
        <property type="entry name" value="CD36"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="976"/>
            <end position="999"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8182113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1041" mass="121228" checksum="55D7DD10D6532573" modified="2022-10-12" version="1">MDKKWKIILSTFTCCTVSFLVLAIITPFVLLSIIKSQAQDEAIMSSSNYALWGQVPGDTKTYLKRDFTFFQFTNPYDVMFRGKKPIFVEKGPYTYQEKQNFTDYKFNDDGNIVTFRAWIQTVGEKQNDKITFANFVALGAWHQLQNTEPAVMALQVFSSFYFELKKFFYPTMIAQAVISSFLNEDTAKKTVLNGLSVEKQNLIWFDQEFGMGNNTAFVNWVQVVKKHTDDSFLRNYFQISQSQMNVIKKQLAPLIQGAEGSIKQLFCTKSPTQECFDAYLCAKQWATQSITNDPDAGTIPVPSVITGNTTSHGYPELSYFYKEYFLKVIEPTNEDYKNLKFTTEWALELLRNYENYSIDDPKYYTDSHLLLHIGNLRYLYEQGNKYDKHKKDDYLMKIQNRFKLESIYHSRVFYEYVKYMEEEFGVQKSINGTKGIAGLGTIVSQALYGEMANLNDNLLNYVLSDLLIKKITKCEQVLEKLDKINDIQKEKFCSLDEYKNWNEKSINKLLYVCEHQESTYWGQLQNLTGITNIQLIQLCDDEEEGFGQELKLANNKMKTKYDCKLERCTKFEIGVKQWAFSMISDNPIDEIYKASKTMGDIYPIFKKFEYTWFQVQFKDIFTDKQLDYDLARYLLSFECLYNGQMISNAFIHFVNDDKTSFQKILPFDDYNILHYLRYVMIELGLEGFADTRTVNEILFGYWPQLTVAIKEMNPAMGGDPSTPITGLNLNIPSELSQLQSVYTGKKDISKVRKYALIDGVNYLNVKVPYFDGNQTTFKQFNPWNIEVACDLGTDAFSYEPQLPAKGHIGTIITDIVKNIYLDYNETIDFHGLKAYRFRNNKNTFDKNPDYHNYKWDGLENMTTIQNAPIMNSPTHFYNGDIKLQEMIEVYKHDNLTDRQWASVWDDSYVIYEPFTGVALSALLNLMVSVEYKKDVLFPCDNYAILPVMSLMRGANWTSDQVDETFGELKSGLKLRWTLAIIFYVIAFIFLICSMTVFYFKYWVPKKMRVQQDESYQNLSSIENQDGQINRSIENQEAQMNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W1F1</accession>
    <name>A0A8S1W1F1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0820178</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000082">
        <property type="protein sequence ID" value="CAD8184178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W1F1"/>
    <dbReference type="OrthoDB" id="305670at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="185"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8184178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="391" mass="45559" checksum="A9E3A4ED80F425F4" modified="2022-10-12" version="1">MLNNELNLLVKKAYRHNLLTPLISQLRKQTIINTRFTDRNNRKKQTIRQASTIDAIQQINCIDQFTGRSSSKLTSSILIVKQERRNTASPSKIKRILSLCQDDNIQQLSDVLGEISRIELETDLKQKSQTLLLRNIRINKQQFIKFLERTYPIIICQQIAKAFKVPPIMSAQEYKQLIDKLEYLDVKSQIRLCFLIYDFNNQGYITTHDLVEILKYNNNQAVEKDLLHMIKSTKTQISNQINNDIIQNNSFILPTSLLRVKLDRSVILQQRRQSLISHDGRGRCISRLSQKNSPDLSVELDQDNNQQNKNITPIKKVVLITQINKMEQNTDSTNNLIQGLQNYRSENQKKKIKIVKDENKISTDLTGFINIWFPNRPNIFQDIIKTLTEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W4F2</accession>
    <name>A0A8S1W4F2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0810053</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000081">
        <property type="protein sequence ID" value="CAD8183547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W4F2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035742912" evidence="2">
        <location>
            <begin position="27"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8183547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="159" mass="19047" checksum="9E9A23D772F7ED29" modified="2022-10-12" version="1">MKQVRIEKQLIFHKLLLILLLKQSQPLPCNNGFLQDEQKLNSKNLLFELLYLLRRQTGKKQKNRKLQKQNGDIVQYDFPFQRIVNLGHMKVKNKDLDVIKIKDLISKKKTEINKLNQRKPKPQKKNKNFLEKENQKSGFVRNSYSPSRIAQNYFREKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WAY5</accession>
    <name>A0A8S1WAY5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0880204</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8187214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WAY5"/>
    <dbReference type="OrthoDB" id="422186at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555:SF170">
        <property type="entry name" value="FI18122P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="1">
        <location>
            <begin position="76"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8187214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="437" mass="52282" checksum="E46D1BCEC1F69B61" modified="2022-10-12" version="1">MSLQQKQNLLVKEIIEKGYNIEAFQKSIDQRHDLEQWDYDQLIEFVKQFQDQQNDYVYILKCNKTIPNAISQIQDVKATVVGYEKIQKGIFKNTSIYFQIETKPTNWVVKRTYDDFILLKTTLNKYFTVPNIPNQRKSPVDFTFIKQLRHLQMFLNFIIADSEIRNLTIIQEFLSTEQFTINQQFNYSNMNGEVNVRINQSIANFIKQSDYFLTNISPIQKKAYKLIKQLMKQMQQKNQTLIQLTDVYKELFRESKAQNTRLKDCYKNLNDLFESSQKLESNQIKIFNETIYPQQRFQYHQTQPLKELIILRDKSLNSYQEFSQQLKQKKEKLFQMGEVIKWDLDESFLDHFKIDQIKSNPKIAFQCMCQNENAQQLQLKNQYGALNQKAYQTIDQIINYTSLQIKEYLEKMLKLMTSSFQQYQTVMIEISNNLIEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WFD4</accession>
    <name>A0A8S1WFD4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPENT_87.1.T0900162</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8188023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="422637at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015910">
        <property type="term" value="P:long-chain fatty acid import into peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042760">
        <property type="term" value="P:very long-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03223">
        <property type="entry name" value="ABCD_peroxisomal_ALDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000636">
        <property type="entry name" value="ATP-binding cassette sub-family D member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000097">
        <property type="entry name" value="Probable serine/threonine-protein kinase nek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050835">
        <property type="entry name" value="ABC_transporter_sub-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384:SF67">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY D MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384">
        <property type="entry name" value="ATP-BINDING CASSETTE, SUB-FAMILY D MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="7"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="208"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8188023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="423" mass="48523" checksum="F89F5E8E556B0D94" modified="2022-10-12" version="1">MSRISDFEILNRLGEGSYSQVYKVIRKSDQQIYAMKKVKLFDLKEKEKENALNEVRILASFDDPNIINYKDAFIDDNMLYIIMEFATQGDLQNKIKQAGNQLLPETXYLKSVNNDASAITRDYVRNSSLLINLAKAIGRLVVSYKEIQQLAGYTTLVAELDEVLKDLINGKYMRTMLQSNENQPGYFAQKQSLVSMNRGQIVETENTIKFEEVPIISPNNDILAQKMTFEIQPNMNCIVTGPNGCGKSSLFRILGGLWPISSGKLYRPHIDKLFYIPQRPYLPAGTLRDQIIYPHTKLQMLRKKVTDDDLTELLRLVHLDYLVVREGGYDKCNDWNDVLSGGEKQRIAMARLFYHKPVFAILDECTSAVSMDVEATLYQTAKMLGITLFTVSHRPSLFKHHDYMIQFDGEQGWNFKKIEHSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WKV5</accession>
    <name>A0A8S1WKV5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">1-alkyl-2-acetylglycerophosphocholine esterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0970130</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000097">
        <property type="protein sequence ID" value="CAD8190814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WKV5"/>
    <dbReference type="OrthoDB" id="2363873at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003847">
        <property type="term" value="F:1-alkyl-2-acetylglycerophosphocholine esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10272">
        <property type="entry name" value="PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10272:SF0">
        <property type="entry name" value="PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03403">
        <property type="entry name" value="PAF-AH_p_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8190814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="470" mass="55274" checksum="E16409376AAEB2E0" modified="2022-10-12" version="1">MKYPYEFQQQDVPLLEMALAILMLGSFMNDLLLILIYNVQIDLTEKSQMIFSTAFIILFMIKCQKMRLTIYLLIPAYITLIISKYSSHQSYIMVFICLIFQIGYIFLVMVMPQYKYPKPSGKYLIGFKEIKLKNGDMVSIYYPTLQKSKIRSINSCRNRDYIKQVYNDFQHVMHWRPPIMFIYWMLGFLNKVRVEDIEVDSPIIEGKLPTIVFSNGLGGLKDHYSVFYKEWASNGYQVYSIQQEEVTIILNEKEIHQYKIGEATLENQLGQQILNKVKMIRHQQLNDRVIKYQQLLDYVYQQQNVDLNKIIGAGHSFGAATVHRTAQIDKRITAGIILFDPWFLPFSEDTLNIKLSVPLLSVNSETFRLRKENETPQRYSIVFNAQSKKLVIMIKGSQHLCTTDLVYKMPFELKMFTKLNNLNKILQITIAHLRLSQYFIESLSPQKTLTKFEEFSKESFRQSIHEIEYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WLP3</accession>
    <name>A0A8S1WLP3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial</fullName>
            <ecNumber evidence="12">7.1.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T0960163</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29091"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="7.1.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 12">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Mitochondrion inner membrane</location>
            <topology evidence="12">Peripheral membrane protein</topology>
            <orientation evidence="12">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the complex I 51 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.2" evidence="12"/>
    <dbReference type="EMBL" id="CAJJDO010000096">
        <property type="protein sequence ID" value="CAD8190523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WLP3"/>
    <dbReference type="OrthoDB" id="10249483at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.230:FF:000001">
        <property type="entry name" value="Mitochondrial NADH dehydrogenase flavoprotein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.600:FF:000001">
        <property type="entry name" value="NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11540:FF:000001">
        <property type="entry name" value="NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050837">
        <property type="entry name" value="ComplexI_51kDa_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001949">
        <property type="entry name" value="NADH-UbQ_OxRdtase_51kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011537">
        <property type="entry name" value="NADH-UbQ_OxRdtase_suF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011538">
        <property type="entry name" value="Nuo51_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019575">
        <property type="entry name" value="Nuop51_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054765">
        <property type="entry name" value="SLBB_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01959">
        <property type="entry name" value="nuoF_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010120">
        <property type="entry name" value="PRK13596.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11780:SF10">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11780">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE FLAVOPROTEIN 1 NDUFV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01512">
        <property type="entry name" value="Complex1_51K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10589">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22461">
        <property type="entry name" value="SLBB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00928">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00645">
        <property type="entry name" value="COMPLEX1_51K_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 12" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 12" id="KW-0288">FMN</keyword>
    <keyword evidence="9 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10 12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11 12" id="KW-0520">NAD</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="12" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NADH-ubiquinone oxidoreductase 51kDa subunit iron-sulphur binding" evidence="13">
        <location>
            <begin position="353"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8190523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="462" mass="51422" checksum="109B55FF315A031E" modified="2022-10-12" version="1">MIIRQAQLFLRLVPQPRYYHGGLKDQDRIFTNVYRDGDPYIQGALKRGDWHRTKDILTMGQDWIIDEIKKSGLRGRGGAGFPSGLKYSFMPKVNPDGRPSYLVINADESEPGTCKDREILRHDPHKLVEGALCVGFAMRARAAYIYCRGEFWVEANALQQAVDEAYKKGFLGKNACGSGYDFDVYVHSGAGAYICGEETGLIESLEGKPGKPRLKPPFPANAGLWGCPTTVTNVETVSVCPTILRRGANWFASFGRPNNRGTKLFCISGHVNNPCTVEEEMSIPLKELIERHCGGVRGGWDNLKCIIPGGSSVPMLPKEICETVLMDFDALRDVRSGLGTAAVIVIDKSTDIIDAILRLSKFYKHESCGQCTPCREGTSWLVDLLERMKVGNADFAEIDQLEELTYQIEGHTICALGDAAAWPVQGLIRSYREEIEERIEDYHAKHPVKSRQLRSHPQSSSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WNU2</accession>
    <name>A0A8S1WNU2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">P-type ATPase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0960049</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000096">
        <property type="protein sequence ID" value="CAD8190301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="296526at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="445"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="485"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1028"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1061"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1111"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1145"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1177"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1220"/>
            <end position="1238"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="6">
        <location>
            <begin position="1009"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="88"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="567"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1261"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="37"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="574"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1275"/>
            <end position="1284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8190301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1313" mass="152139" checksum="6BF9E1A7D8EE44D1" modified="2022-10-12" version="1">MTEEDDNIIAFEEDDDNQPPKAQPKISTRFKHKQVRQQEISNEQAQEASEDGPVINFTKLFKTSKEVGTAQGIKVDPTYVAPMAKSLFLNPDENQEEDDDPGVLKLEDPEDQPLPPEEGDVFIKSKSNRSQRSTPKKDESILDVIQKYDGKNIENSLEFSVHPIGKGTTEQFPASQENRDELVDTIFSQQLKIGIQSTDPKTDFYGISMLTQKGSLFQNALKMSLKRAFMTPILPFIIIITILDYTMTSVNQFQFFIIYILLLATLPTFIWYRVYKQELELEFKFDRQLISVFDFAQKRFSSRTIIRLFPGCIVQLKKGERVPAPMIILSAEGQEHAQVNFGDDVKTYKVAAPKLQEPDLTYIDGQLEFNQLPIFSENTKARLRLDLVEPIDVQLNQENLVDENGIVESSQLIGVLLHSSTRQKYLREALSFNLPLYLNPLNSEFFSIGIAIVLSITFSIAGSIWSSSNKDMHIYIREQSNYNFISLLIDRLIIFSNIVPIFVEVFRPIFLLLARKKVNGDIGMDPQRSGQLTKLNSYIALDNLANTKFVNYGQVLNEPKLRVGKATLLGGPQGEKKDNEKNNDDVKLQGYEPKPNKEIMDDQIQVNQSTAFEQIDLEYDAQCDDKERSTFVFERALLTLPYKTLNPTQLAIQKYCQLGECVQFSYDDNQRSVLLPKEVLRTYTVKLIVNTEQWDAYLYEMPLLEEKDLESDDEEASDLQQLQDRIKKDRELFEKMNSKRYIWVLQADIESTLCKEHPQLSQFMQGKAVFEYTFQEQEYQRLYKQLGSSAFKRKYIQLPPFAEEQLMTQIKPCGVFQFQTVAHHNFHLLLDLNLAIIRQWFFIRDVERDQTLQYLKQYMECIEFTKDDLLNEMNSVKSQAVPLFKQSTREFKVFDNLYKIDDKFITLIINQEVLDQALSDGEIRSQFYEVIMIASFVVFHDLSKVQIEQLITFQKDQSKNQAIVSVGLHDEDLCLCKVNTIRASVQSQSAKNPSLFSAMSHIELVNLDGLSKLILTHARQTHVRTAGFLLFILYKGFLITMCEFFFSFFNGYSSTQLFTTSFHFMFQTYFTPLYGFLIFSFYSDVNHRVIRMFASEFIKCQRDYYFNKKKLFLIFFDSVYQGFIIFYLPANILQYANNSEGIIEGFYYISLVSYLTILLTTQMQPILYLSTLSKEQIVVIMFNITICILILFTTCSSETWSTSIPQYIHHLESILDSVKSVFIVIFLSCISLTPTIILQQFETVSDSQLIRMIHYYEEKEEIEEEPKQKPQDQKLSQPVDTKQLQTKGLEQSIMIEQTSQDQINMSQDNLLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WPI8</accession>
    <name>A0A8S1WPI8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1000062</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000100">
        <property type="protein sequence ID" value="CAD8191884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WPI8"/>
    <dbReference type="OrthoDB" id="303514at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="438"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8191884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="569" mass="67152" checksum="EC66574621B913AB" modified="2022-10-12" version="1">MDIHLTNNNTLQQQDLNQSNYSSQQLYQIALQFLDNPQTLSNALLIFNQIAQQEYNIYPLLQNQFINLLLHSNPDVQNLTISLISNLIHKKYDFSSLNKQVIESLLINIRWDFTLNTPQQGFEYTSILLNAPFPDLNNLIRIVEPTSLIKYYFINKQGININGLLKTMEIFLSNHNFIFNNAIEFIEITQRIARHQNEQVRSYALKYIHHLVNLQENQTFKELIHATLLAGLMDESLEIRIQALNELKTLNPKEKDQLLILLYKNSHCDDLRKVSESVVQQLFPHFSGVIQTELINVINEKRLNECVILCCYSTISRNWRIRRSGLKTLQSIIQYYPYQDIPIIEILKVSQNCSLFEINFDVRYNGFLLMYHIVNMYQNKITQFNDQIIQNCLFHFSDDHIECQELCINILMKLIDDQHLIQELQKQLRLIINKDLILQDLQDKIKKIEDIQKQFENQKNKEEINEEIIIKYQSKIMLGIIVCFLQMIKQRFNEQSLEQISLMIYQGIQFINSSKSIVYKYLINGLKQIYMQDKELVKKMINQLNLIEILRLDGELELIDIVGTSILKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WS66</accession>
    <name>A0A8S1WS66_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0990145</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000099">
        <property type="protein sequence ID" value="CAD8191650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WS66"/>
    <dbReference type="OrthoDB" id="432483at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000123">
        <property type="entry name" value="Potassium channel KAT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47823:SF9">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_10, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47823">
        <property type="entry name" value="ION_TRANS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="147"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="268"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="342"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="6">
        <location>
            <begin position="454"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8191650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="662" mass="78157" checksum="7905BA3D0BA3CBFE" modified="2022-10-12" version="1">MNRSSHVKSGQTAFKVLNFFLRSKKSTQVVPERRHFEHDEIKKIFRRCLIRLRTIYILNQVTKEIVTYGTSSNLYDISTRDRPALQRYLFPLSKSQDQDEEKLEFPIIHPNSLLKQIWNGIFLLMMIYVATILPFRISFYLDNEIQAWYYADIVTDILFWLDLIISCLSGYYDEEGKLVKKRKIIFMNYLKGWFTVDLLSCLPLNYVLDGVISENGNMKSQNIKLFKLLKLPRMYRLFKIIRFIDMMKYLGISEIMEFFQFNYGFSRLLSLLISVCLVIHLSGCLWYYVAAFNDFDSQTWVSRNELTSSTIQTKYIASIYYAFTTLTTVGYGDIHSFSSAEMIITIFLMIVGVGFYSMIIGLLSSILSQIDYKGHKLQQQQAILNEFCIEKKISVNLREKLKETLEYSFDKNCFIWADNKYIFKDLPINLRYDIIMNIHNGVFGNMQLFQLVDDKQFLVKMVPLLKPILFLESEIIWEQNSNPDAIYFIADGRMNFKADFIVIKSTNQKKQFAFKSMIGGSYFGEIEIFLHIKRETVAQCESQCEMYYLTITSFENEIYDDFPHIMNKMNKIAIERRQKNLETIQQLQYFIDDATKKQLKRTQPKKNTVLLREYIKQNSQHTLEDIKPNDSENITDRPQISTNRSKFQDLFLITQEIEQLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WSW4</accession>
    <name>A0A8S1WSW4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1030034</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000103">
        <property type="protein sequence ID" value="CAD8192958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WSW4"/>
    <dbReference type="OrthoDB" id="433211at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40515">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 157"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40515:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 157"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="130"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8192958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="372" mass="44836" checksum="A2AE1626778FC230" modified="2022-10-12" version="1">MFDSESFAIRNSHKSFISGKNYEDVQSQASSPHKLTRINDLKNQVENYLDMLESKVTMVKERALSEFVIAYNGYMRQVKEDLKYLKEKSLEMEKYYKDNKRIQNMEQQLEWFREESVKLYTKIEIKNKDIFELKFRLQEVQKENEFLEEQIKQLMRKNKRYEVIRHTASVATEQAEQIKEQQIFCTQPRPKRIMSGQTQPKHMKQLYDIFERLANDDQNQIINEIAQYVSFIENTQTKQIQILRQKMNQAICQSTKAFATRSEYEEFFIDCVEQVRKDIMRRRQNAQVDNKLSDFNVFRKEDKFKVLELVLMNEKLLQTLHQKVFPTSYVTQLLEPTTIQTEYQDILHQIDKKRSFSTKERMIIKKGKLLYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WTS7</accession>
    <name>A0A8S1WTS7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Beta/gamma crystallin 'Greek key' domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0930056</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000093">
        <property type="protein sequence ID" value="CAD8189146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WTS7"/>
    <dbReference type="OrthoDB" id="282595at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035792652" description="Beta/gamma crystallin 'Greek key' domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="2">
        <location>
            <begin position="546"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8189146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="634" mass="70528" checksum="A39D72C5D0B6D0DC" modified="2022-10-12" version="1">MKNRTVILLLLVCSAVQVLGEPVVVSQSFTGNAFNDADGWVVAGGPAHVTECSGTKMFGGFGKFGARAVASKLFELPPHSLINLKVQFWKIDSWDNEEGYIFVDDQLVWSRRFQYNEGDGQKCGQGGDWKEMTLNLNLNIKHTGATAVVVFTSNLNEAADNESWAIRDFVLSVERCPEGCSACQVDDKPENCQFWQSFSNSWGSLDANQLGADGWDVAGGQNAATNCGGVALFGGFDKTGARSVISKVLKVNPHYKLKIKVLWAKIDSWDNEAAQIKVDGKLVWERRFQWHEGYFGKICGCPVFEWKTIFARTEVDVDHTGDQAKVEFTTTLDEAPNNESYGLRDLYIFYASCSENCAECTGPKDSDCKKCANNWALVGGKCQALPNFILLEQSFLEDKFTGINGWVLNRNKGGKQINECGGKSMVGGFDIMGVGASATKTFDIPPHKRLRLQSTIYKIDSWDGEFMIIKVDGTEVWKTSWNLQTGGANFCGQGIWFDGIVGVDQIFNHQAPKAEVVFTSTLDQDAIDESWGFRDFKLWYEPKEACAIFYSECDYKGASFEFCSKSPDFQKDNIPPQIRSIRVPPQGRVTLYESTDYNGKKVTYTSDQACIQNFDFSLIQMSGNVEGGWIEVEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WVZ7</accession>
    <name>A0A8S1WVZ7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Eukaryotic translation initiation factor 4E</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1020179</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000102">
        <property type="protein sequence ID" value="CAD8192810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WVZ7"/>
    <dbReference type="OrthoDB" id="282246at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF18">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E HOMOLOGOUS PROTEIN, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8192810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="162" mass="19052" checksum="6E4FF0CC2C0596AC" modified="2022-10-12" version="1">MLQLASDFTFWLGTFKQGQYVQEVIGSFSTAEDFWAYYQHIVRPEQQPEGNQIFLFKKNITPVWEDPQNKFGGRFIFKVRKSLGNQILEELLLSFVGADCDYDEFINGIVCHTKKGFIQMALWVKDIKHNMDAFTTIEKWLKESILQLKEDRDIEFAFHPLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X207</accession>
    <name>A0A8S1X207_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1120009</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000112">
        <property type="protein sequence ID" value="CAD8196214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8196214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="208" mass="25257" checksum="8E16DAD4CF2C87EC" modified="2022-10-12" version="1" fragment="single">MIFVENIYIMQIINYTIIHKIQNTNSAIENSFLFKLSSFNQNRLSKPPRQNRRSQFEQHCHYMDMKIYLKCSNLIIKNHSIFAPNNILQQKIQLEGIFQKQKEMFNISINQWILLNIWQFGQTFMKLNILQVILLNIYKYQLVIKQFIQIYGLISNLTKKKKIYITSFEENQRLEIFNVKINYQQQEVQLIQLYSKYNMSYSIYIYXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X3G7</accession>
    <name>A0A8S1X3G7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T1090026</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000109">
        <property type="protein sequence ID" value="CAD8195270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X3G7"/>
    <dbReference type="OrthoDB" id="432647at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000833">
        <property type="entry name" value="AGC family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000735">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033931">
        <property type="entry name" value="PDK1-typ_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF163">
        <property type="entry name" value="3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14593">
        <property type="entry name" value="PH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="18"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8195270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="426" mass="49344" checksum="75FE040B48B1E7BD" modified="2022-10-12" version="1">MNQNQVLSRLSCKDINDYSFVKMLGTGSYSEVFQGKNKTNGEETAIKVIDKVFLAKQNKSHLIYLESNALMSINHPGVIKCYATYESNTKLYLSLELMNWGTFRDFIKGRQLTTTDIQFYAAQLVVILEQVHKKGVVHRDVKPENLMMTQDKFIKLIDFGAILLFKGNDVTKEESNIKFCRKSSFCGTGEYLSPELLDNNCCGPQSDLWALGVMIYELFTGKTPFQSDIEYIMFQNISDKEPNYDLIKDQNAVDFIQMLLTKDAGRRLIEAIENDCDYSILKQHPFLSNVNYETLWQEQKEIKEQPKFERKNILRAKTTLVNDGVQLILSGIVDKHSGVLMFGQYTPRTMQLIQKHFSQELFYYNPYKKKNHQIELINAKCKLGNDGEFVITSNNKKYFFKQREHPATQWVALINNAISYNKQFQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X3Z2</accession>
    <name>A0A8S1X3Z2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Aurora kinase</fullName>
            <ecNumber evidence="9">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T1100099</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="9"/>
    <dbReference type="EMBL" id="CAJJDO010000110">
        <property type="protein sequence ID" value="CAD8195717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X3Z2"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14007">
        <property type="entry name" value="STKc_Aurora"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000673">
        <property type="entry name" value="CAMK family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="54"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="23"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="132"/>
            <end position="134"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="181"/>
            <end position="182"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="8">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8195717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="389" mass="45204" checksum="65B4D86E05623B57" modified="2022-10-12" version="1">MNSNKAFTETHCDSRSLKLNTSISKHDPNCTNMPGSSSKASSSKIQKKSHSDFQPMQQTLGQGSFGWVTLVKEIKTGKLFAMKAMKKEELFKYCTVDNLKREIKIQRKLHHPNIIKLDSYFEDKTNVYLILEYAEEGSLFKKIKKQRRLSEDEAFHYLYQTCLGIEYLHKMKIIHRDIKPENLLLDSKGNIKICDFGWSTEMDNLKKAFCGTIEYMAPEMIKSQSTNYKLDIWCLGVLLYEMVQGKPPFTGKNDQEKCVAILSGQQLKYEEFVSEDCKSLIAMILQPNPFNRPSIQGILNHKWMLSKQKQQSNNDQLNSRSISMLMDEFGHYSPLKSCPTVVSERKTNNKEWEEMKQRSQHFVFHQPQPEQPEQTFLRRVLISLGCINR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XE25</accession>
    <name>A0A8S1XE25_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zinc-finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1210041</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000121">
        <property type="protein sequence ID" value="CAD8199179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XE25"/>
    <dbReference type="OrthoDB" id="298344at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="479"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="145"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8199179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="535" mass="63981" checksum="3CBFDA4DA567A07A" modified="2022-10-12" version="1">MDRNRESMFTSNFWREINEQFFMVLSPINISDIRLFKKKFSNYVNLNCHKLLDKLLRQNNRSKLSQFNIINLKQRISLLFQERQQHSEPIQNLIDEMIDQICFTQLIEGLQQTQDLIKNMSQLSEQQQQIIYHQENQILTNLHQMIDCQDELSQHIQQLEQQLEQLDNSNSIQQLEQHVNEINHSLLYTYTSQILQEHSIKLTRTRIKNHKQELSVLKDLIILHEDYQVAESIGKATFNESSNTITVIFDKYLSQTQIDLNLEHFANLQKLREVPYEFPDQLPGLRSDDVFEMQMCHFCKLMVDVKNLKQCSYNHYQMGLHEYNEDLLICQRYQINAKSQQQYFLDLYSTNYIIENQQIQCKRYFCLKCLKHEFDSYETTQFMWICPLCKGLCSCIRCSRNDTIYKLKRQYLELNGNLEDIYQSSYFEMLVKEKRKLIKNIPIEFINLIKNKQENNEDTLTPKSNLKNKKSITKRVIQKKMKKNDQAQKSHKLTHIESSSSSVKIKKSKEIKKRSLFFNNNNNQNDPSNQQIFIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XEA5</accession>
    <name>A0A8S1XEA5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CAP-Gly domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1210058</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TBCB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000121">
        <property type="protein sequence ID" value="CAD8199213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XEA5"/>
    <dbReference type="OrthoDB" id="2130750at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035371">
        <property type="term" value="C:microtubule plus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043014">
        <property type="term" value="F:alpha-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007023">
        <property type="term" value="P:post-chaperonin tubulin folding pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01789">
        <property type="entry name" value="Ubl_TBCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045172">
        <property type="entry name" value="TBCB_Ubl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916">
        <property type="entry name" value="DYNACTIN 1-RELATED MICROTUBULE-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916:SF85">
        <property type="entry name" value="TUBULIN-FOLDING COFACTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14560">
        <property type="entry name" value="Ubiquitin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CAP-Gly" evidence="4">
        <location>
            <begin position="189"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8199213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="241" mass="27299" checksum="17B7AF7B6EA41C85" modified="2022-10-12" version="1">MLAEPLAYDSTVKLTLTHNISTNKMIDIRFDLMQTIGSIKESIEKRYGSDPKFMQLQLKDKQGNFITYMNDDVKFLGTYGAKDGYEVYIIDNNPNSVLNGLEDVNKVQKYTISDEDYDKLPVNMRKFKQQVLQQQAAGGNLGQNKPEINDNFQEAEALQIAIGSRCQILQGERRGTVRYVGQVPQMGLGYFIGIELDEPTGTNNGNIQGFQYFQCPQKFGIFVRPNELIVGDYPVLDLDEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XMA7</accession>
    <name>A0A8S1XMA7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GTP-binding nuclear protein Ran</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">GTPase Ran</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Ras-like protein TC4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T1280102</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="10">Monomer. Found in a nuclear export complex with RanGTP, exportin and pre-miRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Ran family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000128">
        <property type="protein sequence ID" value="CAD8201612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XMA7"/>
    <dbReference type="OrthoDB" id="440027at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00877">
        <property type="entry name" value="Ran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000369">
        <property type="entry name" value="GTP-binding nuclear protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002041">
        <property type="entry name" value="Ran_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24071:SF0">
        <property type="entry name" value="GTP-BINDING NUCLEAR PROTEIN RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24071">
        <property type="entry name" value="RAN GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51418">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="196"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00065175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00075751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8201612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="220" mass="25144" checksum="72DEF4B74E41740E" modified="2022-10-12" version="1">MAQQQQVQVAAEFKLVVVGDGAVGKTTFVKRHLTGEFEKKYLATQGVEVSQVVFYTTHGPIRLVIWDTAGQEKLGGLREGYYIGAHCAIIMFDVTSRISYKNVPKWHKDLTRICENIPIVLVGNKVDSKDRKVKARQITFHRKVNIQYYDVSAKSNYQYEKPFLYLLRRLANDANLSLVQAMALLPPEIPIDPEYAAQIEKERQNAENQPLPDEEDDEFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XN89</accession>
    <name>A0A8S1XN89_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Histidine kinase/HSP90-like ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1310085</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 90 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000131">
        <property type="protein sequence ID" value="CAD8202531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XN89"/>
    <dbReference type="OrthoDB" id="28737at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16927">
        <property type="entry name" value="HATPase_Hsp90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.790:FF:000014">
        <property type="entry name" value="Heat shock protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000005">
        <property type="entry name" value="Heat shock protein 90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00505">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019805">
        <property type="entry name" value="Heat_shock_protein_90_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001404">
        <property type="entry name" value="Hsp90_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020575">
        <property type="entry name" value="Hsp90_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003555">
        <property type="entry name" value="PRK05218.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11528">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00183">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00298">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035918350" description="Histidine kinase/HSP90-like ATPase domain-containing protein" evidence="7">
        <location>
            <begin position="24"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase/HSP90-like ATPase" evidence="8">
        <location>
            <begin position="61"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="250"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="442"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="814"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1127"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="19"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="253"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="442"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="814"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1145"/>
            <end position="1154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8202531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1170" mass="135536" checksum="7DCE4DBF99D8CA63" modified="2022-10-12" version="1">MHTKQKAFGLLLLFMLSGIQVMTQDLTEEQRKLLEQQQETHEFQAETGRLMDILINSLYTQKEIFLRELISNAADALDKIRFLSVKNPEILGDKTELAIRIEINTEEKTVSVTDSGIGMTKNDLISNLGTIAKSGTTQFIEAIKGGNVNLIGQFGVGFYSCFLAGQKVTVASKNTDDDQYIWESQAAHSFAISKDPRGNTLGRGTQVTIHLKQDAVEFAEESTIKELIKKYSEFINFPIYLKVTREVSKQVEEEPEQQDQQENTDDDEVKVKDDDDDDADTKKKATKTIKEKVSEWVQINENKAIWLRSKEEISDDDYKKFYKVLSKNSGEDPFNWVHFKAEGEVEFTSLIYVPKRAPSDMFDNYYGKQTTNLKLYVRRVLISEEFEDILPRYLSFVKGVIDSDELPLNVNRETLQQLKMLKVISRKIVKKILELFQDAASYDDEDEEDTEEGEEDDNMAETTPEEQQRLKDEKRKKKIDEYNEFWKEYGKNIKLGVIEDSSNRQKLAELTRWYSSKNSTELTSFDDYIERTKPGQDSIYYLAGENKEQLLASPIIQGLLKKGYEVLLLDDPVDEFTFQHLNEYKQKKLVNVGKGDFKQPEDNDEQRKKQKALKKVFQPLTDWWRKLLSESVDSVIISQRLIDDPIIVVSSESGKKATKTIKEKVSEWVQINENKAIWLRPKEEISDDDYKKFYKVLSKNSGEDPFNWVHFKAEGEVEFTSLIYVPKRAPSDMFDNYYGKQTTNLKLYVRRVLISEEFEDILPRYLSFVKGVIDSDELPLNVNRETLQQLKMLKVISRKIVKKILELFQDAASYDDEDEEDTEEGEEDDNMAETTPEEQQRLKDEKRKKKIDEYNEFWKEYGKNIKLGVIEDSSNRQKLAELTRWYSSKNSTELTSFDDYIERTKPGQDSIYYLAGENKEQLLASPIIQGLLKKGYEVLLLDDPVDEFTFQHLNEYKQKKLVNVGKGDFKQPEDNDEQRKKQKALKKVFQPLTDWWRKLLSESVDSVIISQRLIDDPIIVVSSESGYSANMERISKAQAYSSKGGSHQFGKKIVEINPNHQAIQELLQRVKDDPDQETEEMARVLYEAALVNSGYSIPNPEKFASRFYKLFNSALGIDRDAPVKEFEVEIEEEPETSSEPPQSEDGTKWEKVNTDDAQWETVSNDKRDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XQF5</accession>
    <name>A0A8S1XQF5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1320117</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000132">
        <property type="protein sequence ID" value="CAD8202928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XQF5"/>
    <dbReference type="OrthoDB" id="308412at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="374"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="26"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="179"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="264"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="375"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8202928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="482" mass="56858" checksum="0F6898556E6319F2" modified="2022-10-12" version="1">MFVSTNNTPKNNKKGNTSYNDEQRTIERLRFLLKNAIQLNDELEKKNALLLYRIEQQQNQSQQSIQQDDYKQQIVKLQSQLSEAQLENQQLQQSLKNLKQQGNQNPQNNKKNFELILQDCAISQVEKQFYEYFEKSSAILQEQMILEFQNRLEKYQTKYQIVEIHKITSFTNLDKTKCIESQQREIDILESKLNNTEKELNELKRVLESYQQQQSMKQSQQDQVHDFFNSNQFEIFTSRLNNSQGMDECLSNDKRWIDIDNNQLKTDERKLIQYEKTIENQKLEIENLQNKVSNISKKKHQLKLQNQELSTVLYQQAETCQRIISNLQAKIEKQVLQLQYYTSIQQMRNKLNQQQSQHSVQSSQQIDIRKKFTYHSPNANNSPVSIAKSQTNNRSLKKQDSSNDQTTWIIKTTDASLVKKLDKKDSQSESTTDQVLKPTKLSTHLQEVKKSLDQISNVNCTQKLLKGYYFVQNKPILTLDDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XWA2</accession>
    <name>A0A8S1XWA2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1410067</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000141">
        <property type="protein sequence ID" value="CAD8205613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XWA2"/>
    <dbReference type="OrthoDB" id="447506at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052981">
        <property type="entry name" value="Ingression_C2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47052">
        <property type="entry name" value="CONSERVED SERINE PROLINE-RICH PROTEIN (AFU_ORTHOLOGUE AFUA_2G01790)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47052:SF3">
        <property type="entry name" value="INGRESSION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="159"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="198"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="335"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="347"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="400"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="439"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="456"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="477"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8205613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="503" mass="56267" checksum="61EF2BE573090C74" modified="2022-10-12" version="1">MSLVGSGTLIVKHLKAKLTRDTEFLGKMDPYCKVILGSQQQRTREHTDAGKHPSWNQSLSFRRTNEYIAEIQVWDSDEVSTDDLIGECSIALQTYLIDTPKPAEWINLSYKGKSAGQIYISFEWFPDSKYQQQPQIPSNPQYYQQNNVIPMKPPGAPGYQQPYNQSIPYQQSSNYPPQQVQYPQSPPYAQSGLQGTLGYPQNPSQNYNQQGYAPTYPPNQQSGYQPNAQQGYPPNQPPTYPQNQIPNYPPNQPPGYPPNQPPGYPPNQAPVYPPTQAPGYPPNQAPGYPPNQAPGYPPNQPPGYPPNQAPGYPPNQPPGYPPNQPPICPTNQAPGYPPNQPPILPTNQQPNQQGYPNYNQTMQNNPQGFPNQPQNQQNPNHPNTIQNQQPYPNNQPPTNYPGDQHSNSQPQSYPNNQIPPNQQTQTIPQNQPPGQQYPNLPPPNQGYPNVPPSQQGPPQQNYVPPQQQPPAFYPGQTLPQNHNYPSQTLPQNFDPNKIPNPMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XYJ2</accession>
    <name>A0A8S1XYJ2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1370148</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000137">
        <property type="protein sequence ID" value="CAD8204574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XYJ2"/>
    <dbReference type="OrthoDB" id="2017782at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007091">
        <property type="term" value="P:metaphase/anaphase transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558">
        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558:SF13">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 27 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="82"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="116"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="150"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="217"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="251"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="285"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8204574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="411" mass="48280" checksum="9B4CC16B6F86A67F" modified="2022-10-12" version="1">MIKPKVKQMQKKQMPIIGKEMPYKNSECIMNPQKPTLKQLRLNQQMLRHIMAKVYMIKLQSNTQKLLKLIHNFLCLIIIEAIINNYNIGLIYYILLQYDRAVEEYSKAIQIDPQEFISYYNRAIAYHYLKNYDEAIKGFSYVIQINPNYAMAYFNRGNTYLELDLIDDAIEDFNKAIKIDPQITLPKNLEKIAQTNNIYSEALGYFQKAIEQNSQHAKAYNNKGFVQQHLKKYDDAIKDFSDAIKIDPKLATAYNNRGNVYYHLKKYPEAIQDYSSAIKIDPKYVKAYNNRGNAYIKLNRVDDCNKDYQLANKIDPEYKNNKWNVQYDLSMFNMPKQFDQKQIQKDINAIGIDTSITVAYQLKGLTCCMLKDYEKALNNIDINLQNKSQKAQIYFQKGCVLILRHQKEITQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XZY3</accession>
    <name>A0A8S1XZY3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1440117</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000144">
        <property type="protein sequence ID" value="CAD8206605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XZY3"/>
    <dbReference type="OrthoDB" id="307364at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="96"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="612"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="377"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="442"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="21"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="114"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="620"/>
            <end position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8206605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="649" mass="76311" checksum="1317DFFCF2B1B382" modified="2022-10-12" version="1">MDGGGSTFDLTAPVQLMQPKINVQKQQEQTINKGQKELQPQKTQNSNSKLPQLSNNNQNASKSVNQKAMQSGHQNSTKSLQKVKKIELFRDALQQATTKLLPPGKKDKDKTQKPKPLQESQQQQIEEIKKEYEDLVQNHYKDLYAHIGNEQQKSLHDLYNKRIFSALHDLESRPKIQNALERKEEQLNKQRLYKDRLLYEIKQMIIKSRKLDELLNDLAIQNDDTEIKESIEELIQKEQIQEEKMAKQTYEIAKLRDMYGKRRKLLLEVQKQCQITYDGLKIKYQQYEASSQSMMSRKKQLESLSKIVNKYRNEYQNQQNFFGASLKRGFDPMLEEILEDELSNCGDLQALEIVQQGEKKIIAIKEENEKKKKGEQKVKAKLAIENATQNINKQKNDIQQELEKKKQMFQKLRVITSIANQEDMQKYKLNMDLDQQELKNIQEYIGKEIERYERQNEELRTEIKKLKYEQENSILDAQINLDEQEREVTKQQDLLQEKEKQVKKVEKSIDEVTMSLSRIMYQLSGKGLKPKNIEIKRHALVATASTIQLRLERMLTVLSKTQEFLNEESINTNPRYNKVEDFICLNPKSYISQEINENAETNIKFVYKEEDSSDEDCKDMDEVRQQVKSRAQEDKPQVLVQPKKDKKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y160</accession>
    <name>A0A8S1Y160_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HIT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1470120</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000147">
        <property type="protein sequence ID" value="CAD8207503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y160"/>
    <dbReference type="OrthoDB" id="672793at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01276">
        <property type="entry name" value="PKCI_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000005">
        <property type="entry name" value="Histidine triad nucleotide-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001310">
        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23089">
        <property type="entry name" value="HISTIDINE TRIAD HIT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="4">
        <location>
            <begin position="7"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="2 3">
        <location>
            <begin position="102"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="1">
        <location>
            <position position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8207503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="125" mass="13853" checksum="44D25988522DDE45" modified="2022-10-12" version="1">MDAEVTIFDKIISGQIKANIVYQDDLCLAFHDVNPQAPVHILLIPKQRNGLTQLSKAQEHNKEVLGHLLFTVTKIVELVDELKKGFRVVINDGENGGQSVWHLHLHIIGGEQLTWPPGSSGSSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8G6</accession>
    <name>A0A8S1Y8G6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1530114</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000153">
        <property type="protein sequence ID" value="CAD8209197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y8G6"/>
    <dbReference type="OrthoDB" id="312317at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="334"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8209197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="587" mass="68827" checksum="9B6D382AD24E8777" modified="2022-10-12" version="1">MLKLSQNQKVKDQFQDLNKMNLKDLQLDQFIYLCHWIFQKQSLNPFPQSFEFKPIGDLIQESFIYEKHIQDKTPSYYKSLNGTQVLEINEQDLKYRILLYVNGDQQGNIAHIIFSTNQNIDTLFSIPNIQNFNTKNQMQFQQPQFSQYPQSNQPIQKQIFTQSTQINQQQVQNGQLQKDITSLQSNANQKWQLLKNHNAFSQMIFTQQQQQQNPMKKFFQTSVTNSCNFNNFPKESSSQIKLTPNQNQNERQQPITGSIQQFQDQFQNLNLQNQFPKQQDKFNSNLNISTQQVTEEEVVFCTQQINNLEENESINNQVLQSRILTNLIQQKQQNVLDCIICTMKYNLDLDESIVTPCCNKKVHVCCYQQDLDQKAKINMSFEKITCYSCGQSFNNRQDFLKKNISIALFGEIVKRRVLADIPMKCCKCQQAIKASQEILNKQVKLQCFQCNTMLCSLCRQEYHGQNQQNQQCPSLAKEIQKAFQGMPILLCPFCQLIQTKDDKCNHVKCFNCKRDLCSACSVDRIPIVAHGNHYHREGCPDYKPWIVKDKINKTKEFDKIKCQRCKESGKPCEYPMSLEEYRKLKQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YFQ6</accession>
    <name>A0A8S1YFQ6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNase III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1710004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000171">
        <property type="protein sequence ID" value="CAD8212740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YFQ6"/>
    <dbReference type="OrthoDB" id="319369at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14622">
        <property type="entry name" value="Ribonucleas_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase III" evidence="1">
        <location>
            <begin position="20"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8212740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="322" mass="38066" checksum="0E042B712BF8C379" modified="2022-10-12" version="1">MQSNKKDLAFKKLLEFQYPELFKKAMTFKQYQQEYMSRNKDNPLKYGNNENLIEWGSDLIDFYFNDFFTFQDLPNKDHKTFTSYSDIRKQKQLILSEKNLADAAVQLGLKDEMLVSINVNSDLKNNPKIQSQAIKALIGAQYFDKKKDLNLLRKLLITLYQDLIKKQLDESLSLPKQDNFKGEFKEFMDKHPEYIYKLNFQELKNKFDQNQPIYEYELVINDVLPIKRNGEQKKAVEKQVFKDALQLLKTDQFKQLLEDSIKNSSVIIQQSGISESKIESQIHNNLQFVHQLGQLEKSLQQNEKINEKEDFGRLVESFLIKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YFT7</accession>
    <name>A0A8S1YFT7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FHA domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1590012</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000159">
        <property type="protein sequence ID" value="CAD8210344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YFT7"/>
    <dbReference type="OrthoDB" id="5855668at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00060">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051728">
        <property type="entry name" value="RING-FYVE_E3_ubiquitin-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14879">
        <property type="entry name" value="CASPASE REGULATOR, RING FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14879:SF5">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SAM" evidence="4">
        <location>
            <begin position="17"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="161"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="265"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="358"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8210344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="405" mass="46870" checksum="D61BEA60CE6E03C4" modified="2022-10-12" version="1">MDYDSLYLLLPKEPKKWQVSDVIIWLQFIGLGQMEDKFVNCSIDGSILEEITENDLEEELGINQKIIKKKLMNWITTGLKEYSIYLCQMKLNTIKKSPNEEFTLLKNVNACQDKQCQSIINQYDELHNSQNKMNNQFENQLILQPLESQQNNFYCIKQVGAKIGRHSSNQILILEENISRFHAEVTYQDSKFYIKDIGSTTGTYIKIQKRMNLFQDMLIELGSNLFQVTQLEEVSNGIQLGLLVLEGPNSDEQISFHLNQDKNSVTFGRKTNADIAFPEDHHLSNLHAKFYLVDKNVTIEDHSSTNGTWLRLSPDGKPSSIYPLTLEDEMTVIRIGTVNQYLCQLDKLSINYNEYNFCTLCHENERDALCLPCKHNSTCFKCSKNLQLCPICKMKISQQIRIYKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YHF7</accession>
    <name>A0A8S1YHF7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1740011</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000174">
        <property type="protein sequence ID" value="CAD8213109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YHF7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="57" mass="6768" checksum="5FF02F63A2148B81" modified="2022-10-12" version="1">MKIELKEIKIIEQQIKKDSEARIGTIVQVIMQHKIDNQMFNREQRTGSECESSYDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YRP3</accession>
    <name>A0A8S1YRP3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T2200001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000220">
        <property type="protein sequence ID" value="CAD8214514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YRP3"/>
    <dbReference type="OrthoDB" id="10605878at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8214514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="69" mass="8165" checksum="3F48E167CE0B0F41" modified="2022-10-12" version="1">MMLYNISNIVLLQPSFQNLMNFQQMMPINQIQMVQIYLSQAKIQNGYYIFFIDRSCSMSGIRIQKANNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RZT0</accession>
    <name>A0A8S1RZT0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0010389</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RZT0"/>
    <dbReference type="OrthoDB" id="293577at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8131994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="570" mass="68019" checksum="E4FF9CEEEB2EE5ED" modified="2022-10-12" version="1">MIKSRLLQRSSSKLTQSQTSLMNQSMTLINSIRKQSSNFSLDKQTEENGTSKLMKRITQKQTHRSIDDISRLLPLNDNLINVYNDFEDNFKVIRESNKVKQEIPQTIKQEIVQINNFNILKTADGLSLCDLYPEKLNRIQIYRFKFYYFRARIKNKLSPLQIHFTFPNISQNQIYKVFLSTSVEFPTKFNCEQSTSSRSIKIKTKSGTKLFYEDYIFMTLYAESDFIISIHLVFGEFHNMNLNEKQQEPQRYFKYWDDKQSLSPKKDKILQNLDQSRYKSTHKLKEFLKGAEVSAKKMISVVQKGKQIIKEKQEERCIKMLVKSQIQEFRKVEKSILQQKLEKHIQCEYWEQIISLLKICRQAYTILQERKRILRIQAKAKLIVLRIKTKLFIEVQQFGHNPFERCNNKSLLLLKTIAIHLQELSKSRAQKIATDFLKKTLIFQTTLIVHNKMVSKVRMIIKAFKAKRFQKRAFKDRFWRLIKNYFGQNNDTQLFTTSSVKAIQIDKPIMSKVIEQYINKRKLQWSQQYKENNSSDSKEYHRPEIQIFQLPNDLEFQQIMKDYYILKKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S056</accession>
    <name>A0A8S1S056_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Carboxypeptidase</fullName>
            <ecNumber evidence="1">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0020157</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8132960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S056"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000253">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF201">
        <property type="entry name" value="CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035756902" description="Carboxypeptidase" evidence="2">
        <location>
            <begin position="16"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8132960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="464" mass="53106" checksum="AD2DD2AD41E81E58" modified="2022-10-12" version="1">MSYLIVILAILQTFAVNPLEDKTVFPDWGNYSFNTYSGYIPIGSGQRQLHYVFLESQSDPLTDPVVLWLNGGPGCSSLLGLNEEIGPFVMVDDDRVFKKNPYSWNTIANILFIESPAGVGFSINNDSSYIYNDTNTGEDNYQAILSWFQAFKQFQGRAFYIAGESYAGMYIPYTSKAILEGNKVSSLKISLRGIMIGNGLLVSDPKKRFYALQEYFLRRNFLPPTTMNTIRKICQVAPQSIKCLLAQNHFEEVCLGSNINIYNVYGYCKEDTTPDFLKSKYQTQKKVKYPYVSWFEGNRVENKVKDNGAPCTDFGPITEYYNRKDVQEALHIQDQPVLWNACNLQINENYHISEEGSYKILAQLRDDYGQQILIYSGDLDAIVSVVDTEQAILMIPNIRETTPWRPWGNKDLDLAGWVTYYDKLTFAVVRGAGHMVPQDQRQNSFELFQNFIYNLILPEHFEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S271</accession>
    <name>A0A8S1S271_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0020590</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S271"/>
    <dbReference type="OrthoDB" id="298777at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8133747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="917" mass="107608" checksum="396BF5A7166BC521" modified="2022-10-12" version="1">MGIICSKDQNVVKEIPQMITSVEENRLQIIRNDQLSVIQEFQCAIWRNLSLKWKQKKLQISLTQNNDIKYVLNGKSEYMQVQAMKNRDVLKFQASPEVLKKLEKLKYVEKWGNSIPQFKKVQQMTVNWNSQKLIEIGGCYNQDGLKLGLWKELSQYYWTKAQIYEVGEYFNNLRTKRWVFLNYNEIIGGGYYNSDGQKVGKWREIDEFSKQSYVICVGEYKNGKKVGIWDFCLEENRYISQIIGCGQYIDQCEDGTLKNGKWYELGKGFFSYSQILLEGLYKMGKKYGRWNLFIQDTKMYIFELYNNALLKFSGGGSYDEINGQCEVKNGRWVEQSDCYSYYKKMLYAGEYNNNKKIGRWDILFDFDSRYQFKLVGGGTYTLQEGLSSVKNGKWIELNDSFREQSQVTYVGEYNNNNKIGRWDTCYSDFWHTIQIGGGYYCENDSIKIGRWIEQSDDYGEKSNVTYEGEYNNNQKEGKWDIQYYDEFLKKKIKIFGGCYKYAKGLGTIKSGMWVEIRMNLSFQKFFIFQGEYHNNLKIGQWKKLQKYDRIFQICENLNFDQQGKLIYQSGKNSQIMFAGEFKNGKKVGRWDSFYKLFIHNPYKLIGGGQYQEVNNGDSIKIGKWIELSDGFYGQSQVIYNGEYKNGNKVGEWDITYRRKENNSFLYIGGGKYLYDEKVGSIKIGKWRELSERFYSGSQVIYDGNYINGQKFGRWNLRYRKDGLDNFDIIGGGLYDINKELGSIKIGSWIEVNDGFYSEAQFTYNGQYLDNKKFGRWDIMYCGGGSYSIIQSLDSVKTGKWIELSDGFFSKSQIIFQGEYMNGQKYGKWDILFRQKISEPFKQIGGGSYDFEEKLGSIKNGRWVEQTDDYGYGIGLFEAFHIGEYKQGKKVGIWVELRKLKDRMEEGFKQTNEVNYNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S2D6</accession>
    <name>A0A8S1S2D6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0020580</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S2D6"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="14"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="154"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="5">
        <location>
            <begin position="471"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="6">
        <location>
            <begin position="814"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="736"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="869"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8133733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="942" mass="109984" checksum="474BF256D7B823B2" modified="2022-10-12" version="1">MNFQKNIRKFLEKVWLNSIFIANFIQGIWWASNTLWLRQPINYFVFILQLACILIRCFQQFLIAKEYGFNLVIHVVSLLYYVTYFESFLLIESNHHLQVLTNCLLIIFTYMDYLKIAVSKYGLICKLGIPLYLIIRIIYTLINDFSLYDLESLIIIGLLFIKYTIMLQITFNKTFVEKTNKYQLTIKEERDSTPILLNQQIFPQIKSQSKGSQSPQNNFSVFLRDPPSEMKLMQNSNLSNFQKISKSSEKSDHSMSQFYNNLLNLFPQGILILNSQLSVSYMNNKCEKLLDCQGTEKVLEKVKACVDNAKIRDYQWDDQNSFQAQYQSKQFHYHLLKEIICLLQKKNIPIDVLDIMIQPQKYQQLLGTFSDTQLNFMSQVFIYEWFIKSGQNQNSSQKKLKLIIIPTSMTNQQQEYFQSSLLIQSCSKVNFQPTNEIENPILLIIIKNITQKHKFQQLREEQIIHHSLIKSFSHELRTPLNSCQHMLNIIKQQQSEEEFKKCLDIAQNSTALLIHQINDIIDYAAIQSYQFSYHISEFPINQIVQEIEQLYKEQMVLKNIQFKILISKNLISFMMCNDKQRILQILVNLLNNSIKFTKEGGCIHLSFIEKELYNINIEVKDNGIGIAEEQLNLIQSSLYSTIEFGAILKSHIEINKKGLGLSIVAKLAQGLTESQDNQLIIKSIKNEGTIVQFQVDHLELNQNIYQQSSNLFTQQTGKFNQSERLYTFNKIKPDEKGVKQQINSSSKVQDDEENEDQNQETYKATSRLNDFEIQKQILLPISPEYFPNKFISSCKNLDYSKWSQQKLICNKCTHVLVVDDIPFNQIALKMMLKHYNIEADSVFDGFQAIEKVKLKMQQHCQIYQLIFMDIEMPGMDGFQTSKQILKLTQKQSTIVICSAYDTQENYIKGSKLGIDTFLPKPVNQEELEVILSKILNIKELIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S6E4</accession>
    <name>A0A8S1S6E4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0040085</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S6E4"/>
    <dbReference type="OrthoDB" id="10264655at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20533">
        <property type="entry name" value="CYCLIN_CCNL_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21797">
        <property type="entry name" value="CycT2-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0195">Cyclin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="3">
        <location>
            <begin position="89"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="3">
        <location>
            <begin position="190"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="319"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="526"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="319"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="354"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="392"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="403"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="441"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="473"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="529"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="544"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8135163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="582" mass="68192" checksum="A317F054E375EF12" modified="2022-10-12" version="1">MSSKSYDRQKILNIEANVGLTKEQYEKKYLFHRLKERFLHSSDLSLETITPEGHENIYIPMDIQTPTQADGLSYEDEQALRMHGAQICFQACNHLKLPLTTAITSLVIYHRFFAKNSFVDYDYREISMASIYLAGKVEETVLKTWYIASTFSSVFQKQKQTPLDIIIKQEKLILRELGFELFRVSDHPHKFIESFYHFIKVDKQVAQKAWCYLNDSYMTDLCVHFPPQVIAAGALYLALRICNHPMPTQPWWILLEATLDQIEQVAATIYNIYEFEKIDFRQAKRILAKANRVAYVIQHSEIYGIPEKIEKPLETIKQISQQQEQQNNKVEQQKSPVDKKSMKKKSRSNEKKSKDKKNKSEKKKKKDKDKKKEKKDKKKKSRSRSKSNEKKQSKKDKKKNKSKKEQDGQEKYKNKDSEIVIQQDINQIETKMDIETPNDCNNNIKSNNNNIQQSSTGNEQKKDDQLCQIPIVEQTNKEPNPQAQHVINDVQQPQQLHDEKEQLKVQLPLQSKQDLIASIKAILKTQQLQKEKEKDQDKEKDQDQDQDQDQNVEQSPDEQDSEEDEQLKALKLKMLAKKRNDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S747</accession>
    <name>A0A8S1S747_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0040479</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S747"/>
    <dbReference type="OrthoDB" id="10004143at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="13"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8135898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="93" mass="10857" checksum="D66017C0F780DBB0" modified="2022-10-12" version="1">MQQEQPGSECEGYIIIKYLGYGAYAVVKLGRKGHELVAIKFLKIKNEQERPQIIQKLKQEVYILQQLKHPRIIQLIKYSDNLTYIKKNGQIVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SAJ5</accession>
    <name>A0A8S1SAJ5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">dolichyl-phosphate beta-glucosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.117</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPENT_87.1.T0060010</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a di-trans,poly-cis-dolichyl phosphate + UDP-alpha-D-glucose = a di-trans,poly-cis-dolichyl beta-D-glucosyl phosphate + UDP</text>
            <dbReference type="Rhea" id="RHEA:15401"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19502"/>
            <dbReference type="ChEBI" id="CHEBI:57525"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.117"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:15402"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.117" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SAJ5"/>
    <dbReference type="OrthoDB" id="3784at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004581">
        <property type="term" value="F:dolichyl-phosphate beta-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04188">
        <property type="entry name" value="DPG_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035518">
        <property type="entry name" value="DPG_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859:SF91">
        <property type="entry name" value="DOLICHYL-PHOSPHATE BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859">
        <property type="entry name" value="GLYCOSYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="14">
        <location>
            <begin position="64"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8137276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="308" mass="35744" checksum="0F5983E816FC0024" modified="2022-10-12" version="1">MLLEILLFVIFVGLLIGIKWLFTPYTNWKNQQKTVKKDDSIVVICDGKEFKYSTYKNTSDVQLSIIIPSYNEENRLGRTLEATFKHFNEYKYEIIIINDASKDKTLEIAKKYQINNQNFKIITYNRNRGKGGAVRLGMLAAVGEIQLMMDADLATDLNEYEKLSKELIKITQNGLGLVAGSRNHLVKDVVVQRKWYRNILMHCSNFIINTICGVRLKDTQCGFKLFTRNTSAILFRVLHLERWAFDVELFMIAQKYKVPVSELPVKWEDVEGSHLNVVEASIQMARDFLLVRILYLLNIWNFKDDIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SAK0</accession>
    <name>A0A8S1SAK0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0040498</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SAK0"/>
    <dbReference type="OrthoDB" id="10319344at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035849201" evidence="1">
        <location>
            <begin position="18"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8135934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="325" mass="39229" checksum="CB5A27118415B862" modified="2022-10-12" version="1">MLLIILLLINAFAFVHKQEQYLMDLYNKMNAIKEFLSQLEYYTALEKLEIRSKENIEFEEESLVIYKEHYQDFINSFKQQQTFNKNEQEDIKLFLRETFPEVVEKIKRIIADKEKLIEGDIKELAKLKRQQQQEDISQRFEELNYLHKKIQEVLEQNDEQIIYKTLELLESKIMIGSFKQMQSQEVDFFMKSVDKLFREKQNLNKKQEAIQLLSIMSSVISEYLIKMMNDQIESSFDLLDKELFFTESKNTHFNAKEQYINLLSRLDQFHQSIWKQMMDLPQNSYQLEIAEKQFEQALQIGNLAISYHTQMNEMIKAMIERFLQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SBI3</accession>
    <name>A0A8S1SBI3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0050397</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SBI3"/>
    <dbReference type="OrthoDB" id="307505at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="423"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="515"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="567"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="515"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="537"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="567"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="581"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8136890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="689" mass="80385" checksum="25E5C1709A86782B" modified="2022-10-12" version="1">MDNQSDFQDSKDSQLNHKYQNSNGSVLYPESFHSQKQSDQTVQSQAVNGSQLKRKSSTKNKNESFLQSNNQDKPNDYDELSISENDENSFNQKENQADQSNSQDQGNDKIEQTFNQDQSKMLENADDQDFEDLPENDQIKKSDDQSNKVTQITKEENQSNIILKQVPTEITDIKQNEEDIQSNDKILGTQEVTEKFKQVIQSPKTKSRQLSQDDPYQLNKPMMKPQQLSQQDNERSYEKNVETNIKEIYKFYSRQAQTSNKQNTFDQLHQIQQVMTLQKFMYFCKDFELIDLEINNDFIYQHTGQGANKPINKIKYKHKEKENFVVTKLILVEIFKKCSNIQELTYQEFLYALIKIADIIFPVDNSLRALYDYLGVHDPKIYRKKMVVVGKPFNTKDQSEILTQEKLCSRRLVLPAKPKPRAESYKYEPPITNPNNISLQEKPKNSAKNLQKPKEKPNNLIKWEDLGNVSKEFDPKELLLAQDLSDKEDDYYLQEYLITTTEKIKKDESILKIQQKKQQNSKKQQEQQNQADEKSLKQNQNNNTIQDHSQSVPNQQKLFKQLMNQQKTQNLQTTTTQTNQSPIVNSNQSNNQTPLLLSKQKNLIGGRPSLESQSYSQSYKAQQIKANKQQVKTVLNTSIEHNSPTTIRKENDGLNAIKRIEAEKKQREQQIFVAFMKNQEQREKKVNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SEH7</accession>
    <name>A0A8S1SEH7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0070029</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SEH7"/>
    <dbReference type="OrthoDB" id="287589at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8138266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="112" mass="13210" checksum="5C578ECF0975559C" modified="2022-10-12" version="1">MSNEYESKILFAIYRLYKSEQINEDQRGELKDLLITKNNQIMNLVEPYEETLLTIFDNHSDATSLCSQKSYKNLRPSKLDIRPAYRKSLQDISQSINHQSLSTMFKKKKFVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SEM6</accession>
    <name>A0A8S1SEM6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0060139</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SEM6"/>
    <dbReference type="OrthoDB" id="293867at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="106"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8137532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="145" mass="17321" checksum="D6B8709CB5F68D9D" modified="2022-10-12" version="1">MDFTKTQTKDINNPNKNEDDSYVKEMKSRLLIKKHKGAIGEENLGNFYIEDEQDTDRQIRFRLEYSVNFWSALLLAAFVSRSIYPFSVRFVKNRLKLEGFLNIHMAILPFIFLTNGFVLGVYEGLWAKYWFDKEVEFKRKQYIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SGC0</accession>
    <name>A0A8S1SGC0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pre-mRNA polyadenylation factor Fip1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0070379</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FIP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SGC0"/>
    <dbReference type="OrthoDB" id="1917198at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007854">
        <property type="entry name" value="Fip1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05182">
        <property type="entry name" value="Fip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pre-mRNA polyadenylation factor Fip1" evidence="7">
        <location>
            <begin position="51"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="231"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="137"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="231"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="245"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8138920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="254" mass="30857" checksum="30D3995405AA7E76" modified="2022-10-12" version="1">MSDYNKLKLYKLTKPETFSLSFYDAYKEYQEWLNNSNNPQRQPRKSHKWHSNITDYFNYGFNEHTMQIYVHKITQLTETYRKYRLQYAEKCDNSKETIFNSAQEIRRLSKDVYPIDLGGIFIPVDDKVFDFGFKFQVENLYEQYNNQSSNEKLLQNQYHCTGEALIFKSKFISDIYRKQVKDIQDLYKQYKKEHQRDLYLLNTAVKVFPIQKEDDFTNQIAQLVKNLAIKQEKKDRHERRERSRTPKKNRSNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SGD2</accession>
    <name>A0A8S1SGD2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Inosine/uridine-preferring nucleoside hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0080097</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SGD2"/>
    <dbReference type="OrthoDB" id="432381at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008477">
        <property type="term" value="F:purine nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006152">
        <property type="term" value="P:purine nucleoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02651">
        <property type="entry name" value="nuc_hydro_IU_UC_XIUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.245.10:FF:000018">
        <property type="entry name" value="Inosine-uridine preferring nucleoside hydrolase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001910">
        <property type="entry name" value="Inosine/uridine_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023186">
        <property type="entry name" value="IUNH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12304">
        <property type="entry name" value="INOSINE-URIDINE PREFERRING NUCLEOSIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12304:SF4">
        <property type="entry name" value="URIDINE NUCLEOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01156">
        <property type="entry name" value="IU_nuc_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inosine/uridine-preferring nucleoside hydrolase" evidence="1">
        <location>
            <begin position="8"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8139435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="320" mass="35681" checksum="57ED392E77EBC000" modified="2022-10-12" version="1">MELEKVNIWLDCDVGNDDAMAIILALFHPKSNLLGVSTCFGNTCLENCTANTIRILSSVGRSDVPVFRGADCSLRSVKASAEVHGKLGLFGAEKLIQTFEPVQNISLNDLIKKTAGEQKFVIIVTGPQTNIAILLRDHPELIPQISEIIFMGGTSGFGNATPNSEYNIYSDPEAAQFVIDTCKKHKLKLVMIALDLTYTCLLVEPIQQKIQDINTKFSDWCLEMLKEFQAAYKAEEFDFPPIHDPVAVFYALQPELYTTKPLFVAVDCESKLCYGRTVVDKHGILGKEPTLQFARRVVVEEFWNQMIEAIKIASKNSKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SNS0</accession>
    <name>A0A8S1SNS0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0120070</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SNS0"/>
    <dbReference type="OrthoDB" id="412870at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904294">
        <property type="term" value="P:positive regulation of ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16561">
        <property type="entry name" value="RING-HC_RNF213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044235">
        <property type="entry name" value="RNFT1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15860:SF0">
        <property type="entry name" value="LP20373P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15860">
        <property type="entry name" value="UNCHARACTERIZED RING FINGER-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="7"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8143161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="271" mass="31904" checksum="09A31827C9AEA219" modified="2022-10-12" version="1">MSQQENCSICFEDYVDPIKLPCNHIFCRECIVVALEQRQSCPICRRLCCNYINSRFLSITEPEEIMHEHQGEPCFVYRITIHQNTQTQFNFFEKRYVEMITEVLKKNLTFIIDPQYNDNCIRVKLIECVPVHEHSVYTCTVQNIQRLKIIGFSERKIQDSNSKLKYATTQSIIDNFDQETLILYQKLHACIEKIIEILSLNENAKNQILALAGKKDEVQPEQISKHSLELLQTIQMCENGLLKWYYSTDINGRLGVILKHYESYLNYCQNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SPB5</accession>
    <name>A0A8S1SPB5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Centromere protein J C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0100231</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TCP10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SPB5"/>
    <dbReference type="OrthoDB" id="10252174at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.450.20:FF:000005">
        <property type="entry name" value="Lyst-interacting protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026581">
        <property type="entry name" value="TCP10L/CENPJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10331:SF6">
        <property type="entry name" value="SPINDLE ASSEMBLY ABNORMAL 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10331">
        <property type="entry name" value="T COMPLEX PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="256"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="584"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="677"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="970"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1008"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="260"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="589"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="627"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="639"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="677"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="690"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="714"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="747"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="771"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="978"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1011"/>
            <end position="1032"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8141638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1254" mass="148549" checksum="91BF20D22511019F" modified="2022-10-12" version="1">MQQQRSNFGQQIFQNDMTNPNINPNEFMMQNMMKIFQQQMMQMMQSQSSGFDLEKFYHNKVYDMNQFYNQMKPEECQLQQSSKFINTYNPTKYQEVSISFKQETNVNTNRQLHFDDIQLTQNYRNEFHDYNLPENDDFNQYKNKQDQKQRQQRQYEQGDQRSIKQQLQNFDQSDNDDNYIKQYKQQQKKTSNFDDMSIQQANHDQQQIRSNLKKNTLFQQDECDFRQQSNPFDEMPIKPMKNNNIEDMTLRNKKQDDFTKNQQQEIQSYQKQQKQFDDQTNKKTNYSNPFDEIPIQSQKQNQNIFDDIPIKSNKNQAFDDIPIKTNKNQAFDDIPIKTNKNQAFDDIPIKSNKNQAFDDIPIKTNKNQAFDDIPIKTNKNQAFDDIPIKSNKNQAFDDIPIKSNKNQAFDDIPIKTNKNQAFDDIPIKTNKNQAFDDIPIKSNKNQAFDDIPIKSNKNQAFDDIPIKTNKNQAFDDIPIKTNKNQAFDDIPIKSNKNQAFDDIPIKSNKNQAFDDIPIKTNKNQAFDDIPIKTNKNQAFDDIPIKSNKNQAFDDIPIKSNKNQAFDDIPIKTNKNQAFDDIPIKTNKSTFDDIPIKTNKNQDFDEIPPRNNLKKPLDEANQDDQGRVKKKSFLKKGTRQFLSNAQQRSDIAKKEHAEILKENNASGTPLLQNQNNQIKPQRIQQQQQQKVEFKQEQKEVQQRPKKQELIKEQPKQQQARQQQSQVQQKSLKQKEESDINQDSYQEYQFDDNEDWNDEPPKQSKIATSYFGLKKEKDAKEKKQEKAQPQENEEEIVRKYVQDKIDNLNAEIGKFKSENEKVKKAKVKCDDQLKQLQREREEWEKQKEIEKLELEEWKEEEKKKMLREKRIQERQQKTIQNLPNRKEREEIESLKQQVQKLMEEQKQKDQKNKLSYDRLKKQNEELLQRNQELQAEVKALELRLLEQKRPNSQATIQQSKSQQIQLKSNLSGLSQKKEVPQQNNRRQSPTSFGEELQIKHKQSIKEYIKQNSEVDDDNNDEEYQNEDNSDDDQQYNDCRQEQQKRNIQQQNSKKKYDLIEMQNEGIPFTIKNINSLISEQEFNYDQNRFYQGYKKNKDIPSKIINHNVGPDGKISRQYSNGKKEVVFHNGVKREVFPDGYVIVHFTNKDIKQTLPNGTVIYYFADANTTQITISNGPNIYRFSNQQIEIHLTDGTKEIRFGDGTEKYINDSGEEQTFFSDGIIQKINSQKVKQIEYPNGNVDIIYPDGQIQRQFRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SSF4</accession>
    <name>A0A8S1SSF4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0110050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SSF4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8142259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="224" mass="26359" checksum="57886D84CF7DBCA4" modified="2022-10-12" version="1">MSELESWFIAGASTQNYITVCSEQKILGGYYKFGMGTAVQQLFMSHLIIKLELNQQNFWKLGCWENKIFYIIIDELVQKYQFGCLQGTSQCGYSYRDLQSPIKIIIPHQSETLVILFKTNVNNWKNEFWSVNNFYLDILKCVPESLLCNENSSDICKPRMDIPIELIGFLKQMLMYGYQKILCLLNQLFVLIKYLQLEDIRFQGDQNYLQLKLNSIIIIYQNFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SSZ7</accession>
    <name>A0A8S1SSZ7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">YTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0100213</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SSZ7"/>
    <dbReference type="OrthoDB" id="306690at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21134">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007275">
        <property type="entry name" value="YTH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045168">
        <property type="entry name" value="YTH_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357:SF125">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_7, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357">
        <property type="entry name" value="YTH YT521-B HOMOLOGY DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04146">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50882">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YTH" evidence="1">
        <location>
            <begin position="54"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8141602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="263" mass="32129" checksum="FC6F46EC899DB915" modified="2022-10-12" version="1">MNNHFEPYVRNKRDLKVPYISCQDYTNLEQASKLIPALKQINQSDRHLADADNAHTYILRSNNDDDIHKSIKYGIWTSSKENNEKLNAKYLEAQQEGIPVYLFFSVVRSGQFVGVAKLTSGYKEESFQYWWEIKKWKGHFNVQWLYVKDVPNKHFEHLRNSDNIEVTRSRDGVFLSWETGKQMMKIFEEFSDKKSILNDFTVIDEREQALRQYKYQMQQQRNQQQYYPYYPYQYYDYQQYQQYPQHYQQQQQYQNDYWQQPQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1STB8</accession>
    <name>A0A8S1STB8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0110266</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1STB8"/>
    <dbReference type="OrthoDB" id="289926at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="270"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8142646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="416" mass="48527" checksum="6E153AB0D9EE9E26" modified="2022-10-12" version="1">MNKNQIQNTNTRKPSNSYLKTEFSCSSQTKDLRYTTDPSPNRGNKENVVKPSYLKATLQNHLIKQDRNITPNVAKGSVISLFLKQKQEEQHTEIKLNSCQTHKKRLKYEINQQSMCSRCAIDYAIQFGVPIRYNDLDRKMLTFGQDEEHEEFQSFTQNTSKELQYISNIQTQSDGIIKKKELKDFIVKVGDIISQNMQFLKVIQQQSLTLDNPKQQVNKILDELQIMSKALINDLFDKQQTKSQGSLSISNDLPQQINLNSFISVVTQQINDLQNIKKDIMENVDNIITQMDVAPFKIIINKYSEKLKSFEITLFEIQEQYKQLPQQCEQNSFTKKMRDLNFKKKLSKLFNDLLENSVQKPNQLQDQVNNNTKFIQLLQKVNNNQNTNTIFYSSILKDINSDEKPKQIKTRYKPSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1STI7</accession>
    <name>A0A8S1STI7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0110296</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1STI7"/>
    <dbReference type="OrthoDB" id="301601at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8142706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="303" mass="34954" checksum="3CC0382B87630B55" modified="2022-10-12" version="1">MIQQYRGNSQPSRLNQVQTYSRQNNQQMIPFNMFQEFDLMSNKMMQQMDNQLANLGFGSIFRGFGDLENEMMEFSNIHRHMKNLNQMDVNNHAKNGVFQVYSSSYVQSSKLGPDGKIIQEKYFDNNAVARGANGHTISERQQGYKNSDGIDRFAHERMMNDKGRKHIRERDKTGQITTTNNYMNIEEDQVDQFENEWMGVGKNFGINQLTGGLKALQEQQKSTDFNNRPFLKDEISSNKLGKNSYSQNNNPIKQVLQQSNQQLGIKSKVPQSNQPMKALPHQKQEFTGVYQKKGIKNQYPQAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SV71</accession>
    <name>A0A8S1SV71_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0110210</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SV71"/>
    <dbReference type="OrthoDB" id="424834at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052187">
        <property type="entry name" value="MFSD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23512">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23512:SF3">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="156"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="281"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="307"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="347"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="372"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="408"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="30"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8142534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="466" mass="51815" checksum="5DBEA33C5A4DC02A" modified="2022-10-12" version="1">MNESENKDQSQELITNNQKSWHHTNVRWLALILACLFQFGSYFSTDYPSVLAEEIKDSFGKSQSDVNLLYTYYSLPNVILPLLGGLFIDAIGIRLAMVGFFAILIVGQFLCYISAVTGNFTIMLIGRLVFGLGGENCVVAQSYIVSKWFFGSELAFALGLNITFARLGSVLGAILLGKTYIWFGDSLSASMFFCLALLILAWIDAIVLALLDQFCDKRDNVEAKIEGDKITLSDIKEFKLDFHLLTLSCLACYSAFFLFQYNNVEMFKLIYHMSPNQANNIYSIPYYAAALLTPVFGFVIDKFGRRTHLLVLCGCLLVLVNIMFIMITCDSQNACLTAPILGQILNGVYYSLYAAVMWPCVPLCVPERAVGTAFGVVNAVQNIGLTVFPVIVGHLISDETPTAYRHMLLFLGSTAIIGLISNIGLWFVDKQNGGKMAKPSLKDNSQVQDLNAQQEYYYPNSEPKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SW89</accession>
    <name>A0A8S1SW89_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0130185</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SW89"/>
    <dbReference type="OrthoDB" id="309290at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="27"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="261"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8144350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="324" mass="38886" checksum="406CA316F611A776" modified="2022-10-12" version="1">MNSNFLKQIEEENRILEQELQNLLNQKRHRVEKEYEDPEEDNNPNFRSEKGDLSKIDEETDEEQPNRIEPHRLMESPQKPESIIDNIQDSINNQSEIRSSSIVDNQSNRQSQKNVDQKNKQINSLEMRLLGTREALKCLEKEVKEKNAIIQNLEIDLAQKQKQIDFLKKITHKTNTPPKKEFYNDGLQEIKNKCKEWERRYIESNGQFKEYQQYTSKLQELNQQLMYKLKQFESEKLLSKSELDQQKNINNEMSETNTNILIKYEQIIKSQQEQILQQEDKYRILNDKYDELKQNSEQFLLSSRDLKYQYEHMLNTLQSIANSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T3X5</accession>
    <name>A0A8S1T3X5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0180082</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T3X5"/>
    <dbReference type="OrthoDB" id="296831at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF179">
        <property type="entry name" value="OS10G0565000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="12"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8148471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="334" mass="37947" checksum="A32061A0FA8D0AC4" modified="2022-10-12" version="1">MGATCSNSCCTNDQEINSKGGNDKQEVVLSKNKSLKESQQRNNKETERFNADNDQGKSDASIGSMKKENLLIQSTIGKMQRVQLEEITFSDGTTYKGEWMNGLKDGQGVLKWPSGSIYSGSFLEGKLSGKGKLILEDEDFYEGEWKDDKCNGFGVYMCKNGARYEGNWKNDKQHGKGKEIWQDGNTYEGFYNDGRKHGQGILKFSNGTTYEGDFSNNELEGQGTMVWEDKRVYKGQWKKSKMNGYGVLMFPDGRVFKGHFQDDKKNGVGEFTWNDGKKMISNWNNGKQNGIGICTNGDRKIGYWEEGKRTKWLDEQEKKLHSESIQQLELVKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TAL5</accession>
    <name>A0A8S1TAL5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Beta-catenin-like protein 1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0190130</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TAL5"/>
    <dbReference type="OrthoDB" id="1898821at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039678">
        <property type="entry name" value="CTNNBL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013180">
        <property type="entry name" value="CTNNBL1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14978:SF0">
        <property type="entry name" value="BETA-CATENIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14978">
        <property type="entry name" value="BETA-CATENIN-LIKE PROTEIN 1 NUCLEAR ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08216">
        <property type="entry name" value="CTNNBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01156">
        <property type="entry name" value="DUF1716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta-catenin-like protein 1 N-terminal" evidence="7">
        <location>
            <begin position="13"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8149480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="497" mass="58800" checksum="20A619074CCF84E7" modified="2022-10-12" version="1">MDPRKKVKVQQYEDEEEMEYDERNDQQQDMQFREQNIELFDERQLREMIFQFENNYKLNSDLRMRYPNQPDKFIDSEVDLDEDLRKLLILPAYPQLYPIFYSSNAIQIVIQLLLHNNLSISYDVIQFLKELVAPDGEVEPKIILQLYDCLIRNYLIESLGELIVKNEEKTQEEVNLLHDCFEVIENMVDIKSSISKEIGIRSKIIQYLVKRVEDNSQTLDDNRLYASELLVILTQQTKENLEIIGRSGGIESLLMVLNQYRKKDPESAEEKEILNNVYDLLTLLLIQEENQDYFQSYEGLQLMIKIIQGKTSSSIRAYSLILLAINNHKENCQKLIQIGGLSIIFPILERKGLKHKDQNKQFEIDETTVQIIKQLLRRNTDITFKQRIFEKCVEKLESLKKLRNEYVEVIPLIDPEEYDNLDENQLQKIYFDLLGKGLIVIESIDVILLELIYKDLEVQIDKQEIISSVKQMLKYTGEKEDIEYYNPLLQKFEVNLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TBM8</accession>
    <name>A0A8S1TBM8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0180365</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8149017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="308635at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="113"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="438"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="511"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="614"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1113"/>
            <end position="1256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8149017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1391" mass="168345" checksum="6F902FB42AE31C02" modified="2022-10-12" version="1">MQQQYPIQHIKDKYMINMNGNNILQGSSRLSLQYDERPTEQRKREEQKQEIFYYRDLCEQLNKENLLLKSFQIENNELKQKQLLINVELSAAQQELSRIRNQFGSQQSTDKYVQKIQQDNVNLQQQINELNKYKQNLETELKLFKENRLTQTIEIQDTTFSQYQKKIKEMEEDIQILINDYSQSQEKLEKALNEQKQQSKYDIDQMKSQIQLLSAQIQGWQQRYENLELMKNELENKLIILEKENQQLVYKSRYDESQANLDVVSQFKDQIYQQNKQITNLKAELQQKSQSESQLIKEQQNLKQKLDQFQNYTNNYSQIENQEQKNEISKLKQLIEQFQLEIENLRTKQSNLYQNQTKLSQKEVQQQINSYLEEIERLKSELFFQQEQHTKQLKDQENIYQIELKSVNDKCILLQKEIYQKSQSQDESRFTFQTQLEVNNQEAQIDKLKRDIQQLIEQRQEDDIQIKFWQDKFCEQQTQNEDLMYKYQELRNQISQYLIYKTQNEYDQITIKNLEALVEEQQNIMHSLYQKELNYKETITQYQKKIEELQFKNNNESENQLQQLQFQIDDLKIKIRELEAQLNIKSTKCFDLETQIEIEKLKFSERETLFSQQSKQDKKRIQQLELQLENSQKDITLLNQKKNEVIKEYIQVENQQDQERIILLEQSLQNLQQEYLIMKEQYIGLQNQYNIQIMKDQLNQGLQDDKNKIKQLEEQLKILKEQYFELNTLKQQVLKEVVKEIIELPSQSDQILIKQLRQELQQLQSEYMILNTQKQNYIEIQSEIDKNRIKELEKQLITLRTDYDEVCNRKQSPITEYVEGSSKQDLLRIQQFEQMVQQLQDENFKLKIQYTEQIKKSDIINQDLEYIQELQKQLTSLKQENKNLQFQLNQQNESLRKSQSQEFLTIQQLEQKIRILEQNNLELSNKQSQIKVVKETIEVSKDEDIFKISQLELQLQLKTDDIQYLEKQLSLQKKNVEELISANEKMKYENTKMRQQLYELQSSVDDMQLKIKEYNQVQEEIIQYKQMISQYEQRDKFKNDSQEVISELKRKLNESENQIKRQWGEFELSKQDYEFKIKSQETKNNQQIQQLQQEFQLKQKQKDDEYLICKNNCKQIQMQNDEIQKEINRLSDLIKQKNDEIKKQNEKIQSYTVKLEQHYSNLKENQTQIQDYAQQQEKMTQLMQHCNKLENALKQFNEEKKIENLELHEQFNKKIMEIQDIIQKKDNEIQQLSNYIANQQEQIQNQENNKNLITKQNDNQQFLIDLQNQLSIVKQELLRQTTLKEEYQNKNNELILKIAEYEKGNRISIKPDYQRTEKLQGSSSFRNLATATIDFNQNDSQFQNVSQMRIRESTRLQNSKPNNFNPLNSFVNIYLQIEQIIKQQFFTQKKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TDM7</accession>
    <name>A0A8S1TDM7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0190058</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TDM7"/>
    <dbReference type="OrthoDB" id="305884at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="1">
        <location>
            <begin position="31"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8149336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="995" mass="117819" checksum="CF6B49AC89D7F102" modified="2022-10-12" version="1">MKDCYLPDPEKIKYSFNLILFTKDEQQRNQASEFLRQLEQNEPQFLLSLIHIYENDNTEVLRLQSLLLLKNAITRNWIKASIINGKRANILNEQLKIQVKQSLVKLLIQNIERKERNILNQIIQVIVKYDFPGKFQDLENYFISTLSQISQFDNVVSHPIYDFIRTLKSVQKEVSKNRLMAYKIQQKEFSQKVLPMFTHLWRYVTQLQFQDLSKGSDITCNVLLQKTSQKLDQILSYLIMPFHQEEDLQIISEIFKILLQKASELLKVYQNSDIIKTHLKTLICSLSSLQQLYAISIGYSLQDFLTILQIILLQVEPENRLINIGIASITKILQTHFYYCSNEQFKATIPQPKSQMYIQLREVCNETFQEFFNTHIIFFLERLIVINSSKRNSNIKEILEIENDQKSKTSDDKDDVCESTYILCAECQEQLISRFPQQILNYIIENTQQLLSTNYSVIKTELMDSFFSLFGKAIKIQEKQKLQTVSFIPILNYLFQTENPRNYYRYIMLGKEFIPKFCQSEFEQFFEQTIIIIIKSNDIAIKIAGFDCINQIMISKINRIDYPILLKLEQVISLLHNILKEDDISHYIQIINNFLYQYIYTSLVISTNSNQFENKLLQNQYLINLFNIKTNLVVVNFCEMFKKLLISFSFGNVPNQLIQLAIIFITQNIQNNHQDVLDLYLKLLYEFNKCMNDNTTGIQLQGLFLKNENLLLQNLENQTIQCFILQMINELILLDLLPISKNLYQILASLLIKAQTIIDIEQQVEMKNNCLNVMETIILQQIGKIDASFYGNLILYLIKEFIGFQSILLKSFQFIELKTRVINILNIFFFKEETYFMEIVKPNFPNYDQYFIQWQENSINIINKGNKKLNIITTLNFLKYISQHTLQIFIESILKESLIEIQYYIDYQNEEFREQQIKNRLQYHSLEKVRLNKNRESLRKEEIFERNLDYNKLNIQQLVLATINQVMIQFNLQGMQQIIQDQYLLGQLNLLVDEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TEW6</accession>
    <name>A0A8S1TEW6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0210052</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8150860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TEW6"/>
    <dbReference type="OrthoDB" id="305878at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8150860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="141" mass="17412" checksum="44F87F90E25EC91B" modified="2022-10-12" version="1">MSLIEKQIKTDTDEVIKQLKLLSENLIKLKRIFQGELQGQNEETKFIAENTKFLEQKVRPKIDELYRDLDNYEKKVEEIKKKNIKQGDIKYYYEEFDLQMQRYYDFYKIIWAQVYYFVQRQRILLNKDSEKKQQLIQKLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TJT7</accession>
    <name>A0A8S1TJT7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0210252</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TJT7"/>
    <dbReference type="OrthoDB" id="290889at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="236"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="317"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="6">
        <location>
            <begin position="423"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8151249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="794" mass="95183" checksum="E12C72D483F4AB15" modified="2022-10-12" version="1">MYEMQSFKNMIWKKKGLIITIRIFTFIRKMLANLHSYQFRKLTHQNFKALNDQSAYYRFYANKNYVNSNLTYMDKIRYWFNANNFLNKIKTSYKYTLFSHTINPNNSFKLIFDFILSIMLVINVAYLPMNMSFNIDWPEAEILLLILPDYLFLIHIILQFNTAYYDSGVLRSQRKEIAKHYLKNSCILDCLSMVPLIFNFDQSYFMNFFILLRLNALVDILLFFEENYNMRKNYGTYIDVIKLMSIFLYSSHIFACLFFLIAKTEISDGVQDTWIQINHIQDVPWQQQYVTAMYWGSVTTLTIGYGDILPTTNIERAYVILVALLSSIVFGFTISNIGQIFNNITEQKKTQRHRMSMITSFIQKRGLNKELEIRVRKFFEYYFQIEENRDQECEVLMQQLTEDLRKEVKIDFYKRYLMKQPLIFKTFSEEFINKICLIMHERLLIPEERFLFKGDQVNDLSFIIEGYADIIIEFNKNKQNKLSQIRRLNQSQSIAMNYFFTQSPIPYTIKSKTFTRIVQINLQELQQLLLSYPKDWEKFKKLQQSIRLNECPLIECDICKQTHQLEKCNVLFYTPNSKQIIKKIYSIKQDRSNFIRENRIQYQTFNDKLSIQEGVLSFAIDENYFQKESINEEFVQKYDFVRLRNAELNMENRSIKIAQSSKEEPKILKRRNGVRASIRQVIAESNESSDSDSQNLIQMFKILSYRSSEQIEFKNYPDQILYNEYLNIIKPDVVSYLDKQKEFEHFNPQDNITNVLQRNIEVNKNYFKLKTKKSRRLIKKSTWLDSVKPKKVQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TK50</accession>
    <name>A0A8S1TK50_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0220005</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8151592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="291007at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002895">
        <property type="entry name" value="Paramecium_SA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01508">
        <property type="entry name" value="Paramecium_SA"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00639">
        <property type="entry name" value="PSA"/>
        <property type="match status" value="16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035724320" evidence="5">
        <location>
            <begin position="18"/>
            <end position="2462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2169"/>
            <end position="2192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2265"/>
            <end position="2286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2317"/>
            <end position="2334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2340"/>
            <end position="2358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2370"/>
            <end position="2389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2395"/>
            <end position="2417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8151592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2462" mass="282907" checksum="7AF8AED9075CFDE1" modified="2022-10-12" version="1">MILNIFYYLLFIRLVVIRNSDISKQCVCGHVQSQNECKNSGICIWEKDQCILNSMKSYKVEVNNQNPCRNYSQKDCREQEQCGFYFGQCMDFIDCSIFDKDNCQESSYKCVSDGSKLCEQKLEWQVLFLDCGDREKCTNVTMCEELPIYLTNHSMCKEGLEGCTVSQKGYGCIKQMESCSQYFNNFQCFESKSRKDNCFWDSTRGKCIEKECENLPFTQDYEGKTYLSDCTTNGIHCVKRLQCIDAENKFGCVTDAQGKKCEYHQNQCKIKSCYTAPDSYKTYQQCQDYDNLLDCVTSQNKGCKNRPETCYGYDYEIDCYSIEQQDCIWHNDKCEQRQCYHAPIDYSPKECHQYGNCIGQLNGGCQKTPQLCEEILEEEFCEFNYNKERCIWLEGQCVLLECKKLKLPYYKNHNICQKASQFCTFNSDSFGCADYLCENIGEKEFCTIDSTNTVCVLNSVCIEKQCKTAPPSYDTNQKCEGWMPKCTVNVQLVSDSKILIGCVDKKSSCLLSTQDQCFTTLSGLNCKWDGGSQRCIDQEFSDADPNLHLKNDDCKSFKVFVGPCIIGSSGFGCIKWSDNCNEMISQQQCQLNLQDGTKCFWTGAYCKKQQCLDAPKNNYTNNVQCNTWLNNCIFDHIFGGCKDRPNHIACSSFPNMSIYITHQECFAWNPKCTVISSFIAEGCELKKASCYEFIRERNCKKNINGQICYWDDKEQSCMHEDEDDNGIADCHKRLYGDLTHQDCENFMPKCTLNNIVKSCSNLSYYCDYQQKQQCEITIYQQPCKWDYFNYKCKEVDCIDNITAQTEAECLRFRKNNECQLIIKSNGAYGPGCEKRPTSCGQITDSVICKLTLTLFNDRCYYFNSLCQKVQSHQCEAITESKSNDFCQLYNTDCALQSSGQGCYSFQDCAYLSNNVCNNAIMRQNLKCHLQDKCRYDDTCSNKYLSYSNCDGKKTYFGQLCQYVYQCNSITCSYYCLEQTAQMNLTFQSFSTLSEKREQCQNYSSSYKYDTTCDCCVLLTECSFQVGSQSLCNSSITESQTQCGYNQQTNTCKERRCEHLTSNQVISQLTCFNWKYNCVYDVTGCKTFSGDCTTIVFIQQCYSNSCQWQEGNCVNNVNCDLNTTAVTNHECLLINSRYCRLNYTKGQGCAFTDCNVITSSNICISAQVVDGQKCKWLSDINQCTNKQCSEFTIQSDCENSYGYISTTVTKCYWCSLYTSQCSNHSYCNSNCNSVNVLSTIQFTISNKCTVKLQRCSDYTYQEACEKTIDGLDCYWYSNVCLNICEAAIYLISSHTNSSCHSFNASCMQLNSYECNILDCYSLITSADCAIFTEKCFWDGSICQSLGDCSKYSTSTLCLNNINSQGIPCFWNDTQCLEKTCSNKPSPSQNQSECDSWLVNCQWNINNNQCMEDCTQTNISNNTHQLCESFHLNQSCTVKLDLIQCVDLPLSCPLAKKTQCYLDQNGNECYYQLSSGQCVNLTCSNLPISFQNHEKCNQSLKSCTVNETLNGCQQLNECSKYSIQEQCQIDSNNIECEWIISENKCTIKKCTTAQLKIYSAHSCQQYFGDSCTVNANFNGCEISQALCMKYTNTQCQSEGQKNLSGVNCFWNEDKSICLERICENGPSLAQSHSDCEGFLSTCQKGGCRTKNCFDYNYSLDSACASIFEDKRCVTNGYQCVLRKSCEDVATIDGCTFDINLHPCVWIDEKCYTKTCLTAQVQFITHQECNSYLSICTVKQDGGCTQKQNCSDYQIKEACQTDSENFECIWDINLQKCFSSQCVNFCGDGIVTSQNEQCDDGNDIPYDGCYQCEFQCSEGCIECLLSQCQKCDEYYTLDIQTTKCLKEKNYFDDNDLDIEIQKLQSYTNLRCGENQKLIDHLCVNQCGNGVLINQYEECDDGNNYGGDGCSTYCNIEDSYQCISQQSSLSLCTFIKAPEFNLKIVSDNMKQNQIVELTFTELVKMKDSLNFEQLIVFTIIPQTRYQLTISTISNLTTTLSYPKYLVQIEFIEPTQNPILQVDIHENSIFNSYELVLQKNQKTINLETPFVLSGTTKQQLISIVQLNDAMMYSLAGVSSLALVTGNPIILFNLLDLLQSLSYLRFMQYKFPPHLYAFLNTYTKISLQPIFNYFHIDQFLTNLNQGQKQNQIDETSYQDTINILKQMYLLNVKSCYFSVFASILAYMTCNILVSNFFFNIVDKITYRYQDNLKILKLINKFQKTIQKNCLLFKIEYFSLGVFKVFQAILHQTIFSLLIEFPNYPFNSPIEIFSSVNAVFGLLFLLKTIYPFFSITSTQIKNYNKWKYFFYESKSPFWAAQYKSFQIFRVIFFILIIVKLINYPEAQSILISMQSTFFGIYLIRFQPLKSKFELSKIIFKEFLLMLITGTFLLYSFQFSQDHLIIIGWVHIGLFCTILALNLFLDLLQQILKVYNSYLKQKQKKIFEEERKQYFNQLQEFPINNNNYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TST4</accession>
    <name>A0A8S1TST4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0270286</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18579">
        <property type="entry name" value="ABC_6TM_ABCC_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18580">
        <property type="entry name" value="ABC_6TM_ABCC_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000080">
        <property type="entry name" value="ABC transporter C family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000063">
        <property type="entry name" value="Multidrug resistance protein ABC transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000610">
        <property type="entry name" value="Multidrug resistance-associated ABC transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002673">
        <property type="entry name" value="Multispecific organic anion transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044746">
        <property type="entry name" value="ABCC_6TM_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044726">
        <property type="entry name" value="ABCC_6TM_D2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF456">
        <property type="entry name" value="MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN LETHAL(2)03659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="142"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="322"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="362"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="762"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="816"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="895"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="920"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1002"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="105"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="472"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="777"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="1107"/>
            <end position="1340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8155775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1348" mass="153881" checksum="15D7C7FCBE6982E4" modified="2022-10-12" version="1">MNQVSSSVSLKAPLLNEGEVDATRLVKKSRRNRAGVISQLFFAWVYGTIEIASKVTLENDMIEDLRFEDTSEQLYYRFMKQFEKRKNEKNGLIWSLISVSMGQCIFVFIVMLFNVGTSLINPLLIKWTIQYLMKEDKETQEGLILIFSIIGVRIISVICQQHSFYQIRTVGYDWMGILSMALLGKSMNVSYQSNKEHTSGQVLNYMQVDAMKLQWFGWYMSQIMLMPLQIAISIYMMFKFIGVAFLGGLGVILLTAIFNIFVGKKMMEYQIMMMKDKDKRTNCANEIFQQIKFIKVNAYEEYFRSKLTKLRNQEIKTLKTRFFASCLNILSVWLSPMLILNATFVIYVAIGNNLTPANTFAIISLFQSLQGPLLFLPMALNALIEANISFKRVQGFLLTKELMNDCITNSSQSQLDLQYQKGLTVNDSRTQINSQVMRTEIDDDVAIKIDQGTFYWSQYKEQPQQPAKAHTAKGQKVEPLPQIESDPILKNINLRIEKGQFVAIVGDVGSGKSSLIQAMLGEMIYKEDKPRIQINGSFAYVSQKAWIQNATVKENILFGLPFDQTKYDEAIKFSCLKEDIKILVKGDQTMIGEKGVNLSGGQKARVSLARAIYSNCDIYLLDDPISAVDVHVGKFIIYECLNGYLKDKTRILVTHALNYCQYTDYVYLMDNGTIAEQGTFADIKQSEQFKKVYQKFYKDVKNDEESQEQINEVEQLPVSELKLEKKQSSQKETPTVPQNKDEIDELMLLEDRNKGSISFEILATYIRLTGGVLFASFMVFMMLLWDGCYVGSSLWMAHWTQQASEDLANGVDTNNYFYLTIYSVLSLSYGILAFLRSWALVIVSCNQANNMHNKMVSCLMYAPQCQFFERVPLGRIMNRLTKDQNVLDSELQWTFNWMLVQVFLLLANTFLNIYTSSPWVAIPMVIYFFLCWKIQRIYMAASRELFRLEAISKSPILSYFSESIMGITTIRAFQRQSQIMNKHGNNQDLNRKIFLEQIAANAWFGLVLGLSSFMVNTTAIVFCMFYSTKNPAYAGLLMTYASTLDQNINGTVQCLGHVENGLISFERCVAYTKVKPEKGYEAAVKRYQNNQTYRDQYIPQWPKNGIIEYKNYSVQYREGLPLALKNMSIVINPREKIGIVSRTGAGKSTITLTILRILEGLNGQLLIDGHDISTISLRQLRESITMIMQDPTLFSGTIRDNIDPLNLRTDEEVLQAINKCCLTELIESRKGLESNINDHGDNLSAGEKQLVCIARAVLKKSPIVLIDEATANIDIDTEHKIQDTIQNAFSDCTVITIAHRINTILHCDKILVLDKGEVKEFGYTKDLLNQTNSLFYGIYQEALKEQSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TWC1</accession>
    <name>A0A8S1TWC1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATXR3 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0270272</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TWC1"/>
    <dbReference type="OrthoDB" id="308383at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045606">
        <property type="entry name" value="ATXR3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46655">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE ATXR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46655:SF1">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE ATXR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19633">
        <property type="entry name" value="SDG2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATXR3 C-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8155747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="253" mass="29969" checksum="A26370BD9BAB09D9" modified="2022-10-12" version="1">MMAFTYTYFKPYEYVGFLSPPIEDLEWRKVGAFKKKCKSEGRAYSSQFVWGQLIGWFKQTVAAPQASLSQDRRGTLSYPAISSFDKAGDKAYPFQQSKAQSSRSFFIQHLLDKPSYMWPPETASWSYKNTYKIYGTILFEQFYSTELEEDFLDEVLANHTLEYDQKFQLFVKYESKKFKHSIDLFDKFKDIFQPYIGDNQLNYKDMIVAPLRYRRCKFENNSKRESYIENLRSEGKSEEADLIQKFPFISYAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U0X7</accession>
    <name>A0A8S1U0X7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0310275</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U0X7"/>
    <dbReference type="OrthoDB" id="282237at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8158310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="277" mass="33440" checksum="FBF7F3268CE82E4E" modified="2022-10-12" version="1">MQIIKSRAVQQLVHRSQYNKIVWQSSNPYNKRWQYKFKNAYYTYPRDINQEHTYVKTPKDNWESVPLGWAWIQDLLHRHVPNFSCLIERRHRLFDKFNVYFLPAFSLFFYQFYPLAFGFKVLTVLPLAMLYVRARDKCGDPDFKETYLRDMLYKNNEINALFKDETLHVLDYDCEYDKGYPCPEKFPEFKNKFWQFFNTDTSMTTGYFKMADVESGAVMNLKFKTMPVPGKYRYQIGEPFYFYDLRAEITHNGQHKEVVLVDEKVALQKIRPFLLII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U129</accession>
    <name>A0A8S1U129_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0300111</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000030">
        <property type="protein sequence ID" value="CAD8157357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U129"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000077">
        <property type="entry name" value="Centrin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF48">
        <property type="entry name" value="CENTRIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="26"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="62"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="99"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="135"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8157357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="170" mass="19953" checksum="AB13FE30FB00C800" modified="2022-10-12" version="1">MASFKKQQQKTQAPQKKKVKSELTDDQKQEIKEAFDLFDTDRSGAIDCHEIKVILRALGFEVKKQEVQALMKEYDRDETGRIEYSDYIELMTRKYCERDPQDEIFRAFKLFDDDNSGKITLRKLKKVSKELGETLSDQELQAMIDEFDKDGDGQINIDEFLSIMKQTTIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U2K1</accession>
    <name>A0A8S1U2K1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0290226</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U2K1"/>
    <dbReference type="OrthoDB" id="408387at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8156876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="145" mass="17318" checksum="331A766C476E903F" modified="2022-10-12" version="1">MGTCSNRTEHKQISENLRLKAIMIFQKIDIYNKGYIDKERTLQFWKSNFAKLNTEALFSQVDYKKSGNITVQEWLAFWDIVKQQGYSEQEISDELDQLQEGKAWVQFYKVDAYIRADRERHKTQIDSIVQMEKKTLQKSKSFNYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U5S4</accession>
    <name>A0A8S1U5S4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0330125</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U5S4"/>
    <dbReference type="OrthoDB" id="298012at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="614"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8159423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="646" mass="77091" checksum="6F4375BAC3375EFE" modified="2022-10-12" version="1">MQGNQGSQKKDIEFGSCPFSEAMKPPKGHEQYTDKSKCPFQQMQQQPEIKIADGSAEKKKEKKEKQPKGGCPFMSSEKKRNPPLAHLEEQYDTYYISPLNYLLDTRGLWMLAFDSKEVKKGPIKDRRKVFDTYPTYLKSTLFHDDENTKKLRQCEVAQRFFVYDKFREKGNKLLQKQEYDEAIRYYERALGCFRYLEVVEPPEEESDEEETNQQQDTSNMTEKERKELEDMKKSAKQYRKEQKEFKKQYKSLMTIYTDENVKYNGVEHIQDEADKEMCNSIMYGLYLNMSVCYMKMSHFDLARKILDDAGVIQKENSQYLFRYSQAILYDKWSTYKDLLKAKDLIEKAISLSNVENIFKQGPGILKLMGLENAKEIYVEHAHRVMEALKKKKQQVQEIVEPLFLRAKEIDEIEQEMIEDGKVPYEEGVTDVVDPEDLIQQPNETDKQFLYRVCMPQLTQEHQEYEIVKEMVNKYYRIIEFYAEQKKYDQVKIAKQELQRLLETVQTMSFYMNLDLIDYENDEILKELVIKHQLNFTNKKYVRRLIRLCRENVTEIFGQGKFNFEVFEYAMTDYFKKKREQEEKEREEYLKQHPEPVKPKQESSFLKKTLFSTEFWMQMLVLLLVMGGMFYFNSNTGFLGKLFSLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UBG9</accession>
    <name>A0A8S1UBG9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0380136</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UBG9"/>
    <dbReference type="OrthoDB" id="293930at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8162581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="131" mass="15336" checksum="2B675B7CE3A42F33" modified="2022-10-12" version="1">MRKSQKTGSQQHIKNILTPLIVQQNSYSPEQSLLNKPSNRIGRKTSLIELHRVESESIKLRPQRVLQVVKDAKKQLLSQQNPRNEKKIKPRVETSECQYRGLDFTFAQNFSNKQIILKEFSTNGLRKRFFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UFM3</accession>
    <name>A0A8S1UFM3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0380318</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UFM3"/>
    <dbReference type="OrthoDB" id="282153at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035939202" evidence="1">
        <location>
            <begin position="21"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8162937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="89" mass="9913" checksum="545F14D38BD6CDF4" modified="2022-10-12" version="1">MKVCVFILIIGLVLCNQLNGQNNLRQEYQFAPWSLDCTNRCQSAGGVVCGYETQVCCKSGRCQKGWKSEQCGIWDRIHVLNCVPGPSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ULF6</accession>
    <name>A0A8S1ULF6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trichocyst matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0390189</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ULF6"/>
    <dbReference type="OrthoDB" id="298080at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035893957" description="Trichocyst matrix protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="104"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="223"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8163376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="358" mass="40761" checksum="856305327418FCD8" modified="2022-10-12" version="1">MMKFIALALIATTVLAVQKDTKTILAEIDADNFGNSILSTVQMYLQAKGNAEEILMLLNQLLAGLVDDQNKHDNVIRVDRSACTRIVTDLENSIAYHTAQVSANAQMREDNEKALAEAENDIRQTIQDIESNERTFAQEEANRNKAHETWVRKNGEHDDAIAAVDEATKLVQHLSLGATFAELKPKFEAVQKRLIENESHGALFQPIVTALTELASKVDQKAIQRILQLLSQLRQQLVEARSVLEDTENRQAQRWVEFSTHLSNEHNRLVERKNQLEQAIQTFKTNIDTATHFFEVHTLEVESAQETLDAENEWCALQEQTYETQSTERQRQQEIVERILEHLTEKLTATSQYLGGRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UUC3</accession>
    <name>A0A8S1UUC3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0470116</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000047">
        <property type="protein sequence ID" value="CAD8167432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UUC3"/>
    <dbReference type="OrthoDB" id="10252171at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001034">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="107"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8167432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="528" mass="61444" checksum="4079CE8CC90F538D" modified="2022-10-12" version="1">MHLSPSIQKRDSPNEILKARMEKKPSGRFTFLKTQQEDPRQQQVQNNRDRIKSNLKGQILHILNKPLPVTKDQIEELLDLGKKLDIEINTIDSEFICPNTKEFHENYDILEVLGEGCLGLVKRIIHKTTQDLYAVKIVQTQDDEIIRNMIIEFKRLIKLSHENIVKVHKLYVDFDNSFQSESKAYVVMQLIEGQEMFEVLNSLGHYCEADAKEIFKQLLSAIEYMHRNGICHRDLKPNNILCVNNKKQIKVTDFNVSKFSDSYKEFGDLKDREKIEMWTYTGTVAFSAPEIFTGEGYNQMVDMWSAGCILYSMLSGQLPFNADYLNDLIDNIKEAKISFPIELFEGVSEEAKDLITQLLQKDWALRPHPDQALKHEWFNEDQKLLESQRSLLKRLNHRKNDPRLILKQRHNKRQQSILLGSQQLIINLHSMELQQETRLQQTQQQISNMGSMRLESCPKKSQFSFTAYKVPENELSIYQYLPPSSPDKEIEKVGSFESGMLDPFMQCSGRLFGDDKIKNLEDNSDQAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UV37</accession>
    <name>A0A8S1UV37_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0500072</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8168950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UV37"/>
    <dbReference type="OrthoDB" id="284567at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8168950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="14154" checksum="51E804C9467B8A53" modified="2022-10-12" version="1">MVYGRLIYNNVKDYTPQWFKTIPYQQTVKPSFVRKPQVVSRLNADPKVKAFWRFLGRNVADNPWAWQVYIFANSVIIFSLCYYPWLWVYQFNNKKRTIDYALQQEKEFKAKQDAAQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UYL1</accession>
    <name>A0A8S1UYL1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0510245</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lin-54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000051">
        <property type="protein sequence ID" value="CAD8169781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UYL1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005172">
        <property type="entry name" value="CRC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028307">
        <property type="entry name" value="Lin-54_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446:SF34">
        <property type="entry name" value="PROTEIN LIN-54 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446">
        <property type="entry name" value="TESMIN/TSO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03638">
        <property type="entry name" value="TCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51634">
        <property type="entry name" value="CRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRC" evidence="4">
        <location>
            <begin position="93"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8169781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="242" mass="28123" checksum="CE028AB536EA1F69" modified="2022-10-12" version="1">MKPLMQYFSESDCEVNDQSICQSFRGFFDENSQINRVQSIDDQFEQISVQNSQQFSTKCSSIGEKSHKSELIDDLDDLRCSEIDLNEVNTFQPQIKCQCSKSQCQQSYCECFARGKTCNKHCGCQNCQNKQMNKKLKKIQKRLIKQKRNKQRTPRYLKGCTCGKSKCLNKFCSCHQDGKYCNSGCKCVDCKNIYKFSLKSYKSIENVEMNQMEQKNIVQFIIGSENSSKFEILNTLLKQNYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UZJ9</accession>
    <name>A0A8S1UZJ9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0530090</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UZJ9"/>
    <dbReference type="OrthoDB" id="1700726at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF33">
        <property type="entry name" value="AMP-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="3">
        <location>
            <begin position="67"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8170500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="653" mass="74771" checksum="D5B13587039D5B44" modified="2022-10-12" version="1">MQIKIEYFHPISVAKINETPIYRHPKAKNGYLLPLDNRKMNMQQQLLIQFEKFYDKKTLGTYNEQTQSYEHISYKEVLQIATQLGSSLCQQNSIIEVRDQQNNIIRPVGIYLSNTREWTLIDVACILYGFTSCSFYDTLGIENIPYSMNLTQVSVCFVQASTIGFVASSNLPYLKTIVTVGSQDPHILNKLKDQQKEVITWDDFIQRSNGNIQPYPKLNPQDPLTIVFTSGTTGEPKAAIQTHLNLTSRLAQFEHSETFGFTQDDVYLSYLPLPHTFERVMHLAALSGGAQINYFSGNIQTIARDIQRCRPTYFCGVPRIFNRFYEGIQTQLNSLPSDIQQKFELALAEKIQYYRNTGKTTHDQLDEAFIKTKAIFGGRQRIMITGAAPISAQILEYLKVTLCCQIIEGYGQTETTAASFLTDYYDPVCGHIGGPTTSQEFKLVSVPEMDYLIDQIIDGQKKIRGELCMRGPSIIKSYFNNIQSTKETIDEDGWLYTGDIGEIIDGALKLIDRKKNLFKLSQGEYVSPERIENCYLRVKGISEVVIFGNSICDYCVGVIVPEYTFLKEWADKLNVQGDHQQLCKDKSIRAYVLKLVNQQGKQDNLNRFEQIKNVYLEPRPFVQVGILTETMKMQRHKAKLHYQDIIQFLYQEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V3F8</accession>
    <name>A0A8S1V3F8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0530011</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PIERCE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V3F8"/>
    <dbReference type="OrthoDB" id="288097at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005879">
        <property type="term" value="C:axonemal microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035082">
        <property type="term" value="P:axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026507">
        <property type="entry name" value="PIRC1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20899">
        <property type="entry name" value="PIERCE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20899:SF1">
        <property type="entry name" value="PIERCER OF MICROTUBULE WALL 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14892">
        <property type="entry name" value="PIRC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="68"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="72"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8170342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="173" mass="20114" checksum="132915947C4A8B55" modified="2022-10-12" version="1">MQHPRSTSAHYNTCNSQYGNGWLLHEGKQQVKLNETTVPTYAEIVRSMQDFRRAEKLIKNKLVAQKFGLEQQKQETEEEKQRRKIDNKVPPKPFPVRESEYTKPDQGINVGSPIYETSNMQYGQLNPTKFELIEKFYPRDAKFTQGFLGNTFKFDGLNTSVAFSKTHKALDEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V4Q7</accession>
    <name>A0A8S1V4Q7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0530264</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V4Q7"/>
    <dbReference type="OrthoDB" id="437580at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000070">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000001">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF18">
        <property type="entry name" value="PROTEIN TIS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="16"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="67"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="16"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="67"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8170822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="194" mass="22026" checksum="00B4F3DC90EC3092" modified="2022-10-12" version="1">MHNAVITIPQHGNGRLYKTSICRHFELGNCSIGDKCQFAHGQKELRNPNDPILGKIPTIDSNIVITNYKTVLCKYDQQGFCKNGVNCPYAHGTNEKKQARLAPVQLKQLQENKENGDDENVVKFLNQLTDKLMKNDAFKNDKQVLVQLKQTQTMIEEKHHRDAAEQLSLVLSSPSRSSKQQKAYETVYKSLTLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V7H5</accession>
    <name>A0A8S1V7H5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Translation elongation factor EFG/EF2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0590038</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000059">
        <property type="protein sequence ID" value="CAD8173290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V7H5"/>
    <dbReference type="OrthoDB" id="294200at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01681">
        <property type="entry name" value="aeEF2_snRNP_like_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000029">
        <property type="entry name" value="116 kDa U5 small nuclear ribonucleoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1430.10:FF:000003">
        <property type="entry name" value="Elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000194">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF10">
        <property type="entry name" value="EUKARYOTIC TRANSLATION ELONGATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation elongation factor EFG/EF2" evidence="5">
        <location>
            <begin position="590"/>
            <end position="702"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8173290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="817" mass="94487" checksum="0D3C000294065AC5" modified="2022-10-12" version="1">MIQTKDLKFFSNMNKAMKSQDQIRNIAISGTFDNGQQYLYNILIKKWGVQTNTDLILTDNQQEFDQEFQSQCNIKNLYYQKKINKKEEVEDGYLINLMKSQNNYHKQTEILARLSDGAIIIINFEFYLNNEIETIIRTFLKEQNKIVFFINKIEKAFLQQNLYGEEIYINLNRIIEKITQIIQLYESDRILNPAFGQIIFGSAKQQWGFTCLQFAQLYENKFGIEHRKLAEKLWGDHYFDASTKQWSTMSKDSQPLKRAFVAFILDPILKLSQAIMNGQKDVVYQMIERIGIQLNEDVKQQDGKELLKSILSIWINLADAIMQSCILQIPSPRTAQNYKAASLCKLDKNDKLFESIRDCNPKGPLVIQICLMIPYKQEFISIGRVYSGTIYTGQQIKILGCQQKDGKNQDLFQVIVGQTFCFPQGEPAYLEQIPSGNIVGIKGIDQFIKGTCTITDIQHSIQMTPIQYQQDNLVRITISPVDPIHLTLVIEAIKQLMKLHSTISLQLDPCLILTGNTYHHLQFFMEELINKYLSNVEIKKSNYFISYRETITDISNKNQIKTPNKHNMIEGIAIPLSDNLLNQIESDNQSITFKESLKINSKNWFQNEKLKIMAFGPNNIGPNILVNKSNYEDYQHLSEIEVHLNTTWQWFTKEGALCDEELRGVQINILQYLSHADNIHRGAGQIIPTARRLFYGCQLQAQPRLQEPVFLVEIHSNIYVIDQVYKCLNNAKGIVIEEKSFSETSFQKIKAYVKGPNIFQFHDSLSRMTQNKAYSISSFDHWSLLNSDPLEDNSEANQILQDIRVKKGLSSQIQKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VAP6</accession>
    <name>A0A8S1VAP6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cell division cycle protein 123 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0590199</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDC123 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000059">
        <property type="protein sequence ID" value="CAD8173583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VAP6"/>
    <dbReference type="OrthoDB" id="360540at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009772">
        <property type="entry name" value="CDC123"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15323:SF6">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 123 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15323">
        <property type="entry name" value="D123 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07065">
        <property type="entry name" value="D123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8173583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="288" mass="34510" checksum="E0430FA66E9454A3" modified="2022-10-12" version="1">MNNNMNQFCRINWPKGLKSFKSKTIRLSQDVVNFLKEDGIFLNDAWKKPPLKDNGTKRFPELEQIINEILDDFESVFIKLNWRAPLDCQNTFQDMCFYDLYDIMMALKYSGVIMEMIEDYDELVINQNHPDKCQLVTQPSQGYLLELRKYYKLRPNTEFRCFVKNKKLIAISQKNLYLITDDESVKEKIQNYFNKIVDLIDIDKYVLDVYIDIPPKENIILVDLNPWQEHTRPKLFTYEELEIIQECQLRLVKNKDMIVQEDYSGYRESIDVETLIQQQQQEKQQLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VB52</accession>
    <name>A0A8S1VB52_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0610079</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VB52"/>
    <dbReference type="OrthoDB" id="273181at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001070">
        <property type="term" value="F:starch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002044">
        <property type="entry name" value="CBM20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052832">
        <property type="entry name" value="Starch-Glucan_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46642">
        <property type="entry name" value="DUAL SPECIFICITY PHOSPHATASE, SUBGROUP, CATALYTIC DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46642:SF3">
        <property type="entry name" value="PHOSPHOGLUCAN PHOSPHATASE DSP4, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00686">
        <property type="entry name" value="CBM_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01065">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="1">
        <location>
            <begin position="182"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="2">
        <location>
            <begin position="260"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8174420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="333" mass="39122" checksum="60D855299BBF5955" modified="2022-10-12" version="1">MSCISVQKHVNYGEVILIVGDCHKIQWNVQNGIQMQWNQDDIWSVTLDSCCLPMNYRYVVVKQDNRQIVEWEDGINRVFNSHQDVQDIWGHLRITLKFMNYLHSNITLNLYTQNGRHKIPFNRIEGECISTFQIPNKSIHQLAYKYQSNEKWERGGVRMINTHPIINNVIHVIDQGVDFNLNYNRIFENLYVGSFIYIDEIRILQDMGVNAILNLQTVEDLINKDLSEDYFDELQSQSQSLGIIYIQCPIKDCNKRSYLQNGIHAYQILRKLLDQGKCVYIHCTDGIQRSVQTIILYMVQDLNYSLEQAIELVQVIRPRSKPIREVLQKLLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VEQ9</accession>
    <name>A0A8S1VEQ9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0630010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VEQ9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8175347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="124" mass="14787" checksum="9102E609360757D2" modified="2022-10-12" version="1">MKLICLYLHSQPQLQSIQQYRCYHLRYFNIIFFKIFNLQNKCLSYSVICNIELVKNLRFSLKPFFEEVIRTFSAEGYLKFKSYASKFQGSLATSVFETFQTNPNDIQSIKHQPFSEAEEILKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VGM6</accession>
    <name>A0A8S1VGM6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0630149</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VGM6"/>
    <dbReference type="OrthoDB" id="282581at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8175623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="96" mass="11520" checksum="B0FD82636CC1DD57" modified="2022-10-12" version="1">MTSIIRKFQNETLIKNHVFSFVGFMAGLKLCDYLLFDDKQFQELKEDMEDEFWAHNGEPTQIQPYIVDSVKGGKRKSWIYIMYEKDQLIKKKDEFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VGQ1</accession>
    <name>A0A8S1VGQ1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0630114</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VGQ1"/>
    <dbReference type="OrthoDB" id="306198at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00052">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216:SF174">
        <property type="entry name" value="GH06923P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00027">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50031">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EH" evidence="3">
        <location>
            <begin position="8"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="EH" evidence="3">
        <location>
            <begin position="168"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="261"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="290"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8175555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="450" mass="52599" checksum="5CF1FCCFD23F6FF3" modified="2022-10-12" version="1">MFQYTPQEDAYYRQLWTTFDPQGLGFVEAKGTVQFFKKSRLAVDTLKRIWVESVKNQEGKLYYHEFATALRFIAYCQNGFEFNEQLLNQNRPVPLPVLEGIQQPQQLPQQTISQPQQEQFNNFQSPQNQLPQSNYFQTQPVLNTAQISLQSYVEPKLDVQKYDLPDDQYNRYELCFNQQDKTNQGFIKGDQAQLFFQKSQLPTQILNQLWSLVDIGQKGYLFKNEFIVAVHLIALCRKDIPLPQSLPESLLQLIRRDQQQNQVRSTSQTMPQISSGLPPQPQRVGSQNEIQGLNHQSNYLQNKLQNQTQQIQLANNLDQQKQIIEDKKKDIEFLNQTIQKLQSFYDYLKKEQDELQAQITSLSDQQTQKQHSINILQDSIQNQLQVNQNLQKQLIDLKQSNQLQENQAPTLTTQFQTINIEDDYDYSNYKAKKDKVVSGGNQNTENLPWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VH64</accession>
    <name>A0A8S1VH64_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GMP phosphodiesterase delta subunit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0620167</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8175089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VH64"/>
    <dbReference type="OrthoDB" id="10248777at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042953">
        <property type="term" value="P:lipoprotein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.50.40:FF:000003">
        <property type="entry name" value="UNC119 homologue, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051519">
        <property type="entry name" value="PDE6D_unc-119_myristoyl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008015">
        <property type="entry name" value="PDED_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12951:SF1">
        <property type="entry name" value="PROTEIN UNC-119 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12951">
        <property type="entry name" value="RETINAL PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05351">
        <property type="entry name" value="GMP_PDE_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GMP phosphodiesterase delta subunit" evidence="1">
        <location>
            <begin position="28"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8175089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="187" mass="22230" checksum="EEB13A18CFC7F75B" modified="2022-10-12" version="1">MQAGQQINQEYVRNLKEITKEFLCPISANIYNIQFLKFRIRDMESGQVLFEVERDPNEESIENLPLALQDEARRIKYHFGPQFFELKTIGAQLTFSVGDKVVKNFTIIERHYFRNILLRSYEFQFPFCIPNSTNTWEHIYTIPDIDEDVKQLMIDNPYDTKSDSFYFVGDTLIMHNKAEYDYSPINE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VHP3</accession>
    <name>A0A8S1VHP3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0640061</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8175885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VHP3"/>
    <dbReference type="OrthoDB" id="25149at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8175885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="271" mass="32958" checksum="A3E5AC019F8AE578" modified="2022-10-12" version="1">MLSLLVQIEGLIDSMNIINKLFLIIQSVTLTILNQKFRTIFNHKVAQQLKLIIKMCQILKKLYLIFNHQKNSSNHYFSQVQLNNKDLFSCLIYYQRNSYFQFSEIEQINEINLFSQGNEKRINTKGLKFKIKQNNPIYKFISISNKAIENQIEFDSLDNFFFVIQLKDEYILIYLLAIITSQNNKLSFYQTQVLNHNKQYYYLLFDQYYTQSCDLQFFYNYILQINILLFESYFQFNFPQYYLYLIQLFKEFLKTFWKPFKKKITFVKLPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VNR8</accession>
    <name>A0A8S1VNR8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphatidylinositol-4-phosphate 5-kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0650061</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000065">
        <property type="protein sequence ID" value="CAD8176396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VNR8"/>
    <dbReference type="OrthoDB" id="270720at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 5-kinase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8176396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="390" mass="45165" checksum="A3B3B5447793B647" modified="2022-10-12" version="1">MNPQPQNNDENLMRQQLFINENNKLIEQFKASLVEKKKGNKVQVQKFDNGENYEGEIDGGMRKGLGIYIFGSGDIYFGQWNDSFEGEGTYIYQNGERYKGQFNKGKKEGQGIYYYYLIGAEYNGTWVNDQREGFGKFRYQNGDVYEGNWFRGFKSGQGTLILANGDHFQGEWKNNKKNGYGTYIFVSGSRYEGIWLNDQFHQQGILSFSNGDRYEGIFKNGQKIREGVYKYAIDGSEYQGEWLRDQRNGNGKMKYANGDFYDGFWQKGQRYGKGCYKYNNGDQYEGEFVNDQKHGSGVLKMISGDIYEGEWIQGKKNGKGLYRFANHDIYDGYFVDGLRQGQGRYQWNDNSYYVGNWDKDRMNGKGLYMSHDGVQVDGIFDNDRYVGSAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VS56</accession>
    <name>A0A8S1VS56_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0710014</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000071">
        <property type="protein sequence ID" value="CAD8178975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VS56"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009190">
        <property type="term" value="P:cyclic nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43336:SF3">
        <property type="entry name" value="GUANYLATE CYCLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43336">
        <property type="entry name" value="OXYGEN SENSOR HISTIDINE KINASE RESPONSE REGULATOR DEVS/DOSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="381"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="516"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="8">
        <location>
            <begin position="648"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="34"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="299"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="335"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="569"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="74"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="119"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="348"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8178975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="994" mass="112628" checksum="63EF46B2CB1E80BA" modified="2022-10-12" version="1">MNMQNEQDKLVHEEQGLGEVFVPSQVVKNFNEMVSDPNVLQLPSDRSAKRSSENQGQNQAPLLTDKQTAQPLIRKKEPPPDELKKLNKQMNGSNHHSQDDVSIKSSGGGKSPVQQTTQRRVKRMNSKLKSRHHVQKQQSDLAKKILKITESTPFSIIMAIVTIYALFGDDIRILSVDKQGDDVFFILTVICMVAFLAEIILTSICKPEYIFNFYFVLDIISTITMILDLGWITDTWYSGDLSSAAQIKSIGTASRAARKAARVIRVIRLVRLVKLYKHARQQMEKEQQKKMLQELLRQAQQNKSQDQQAPQQPQIQAQSNQQVVPIVSNIEEQRVSQNDIESSRTPDKNSTGSNLKTSQTTSQAEDQIKESVVGAQLSDLVMRRVITIILAILISIPVLSLDTYQEMISSYDSGIYRIYQFRNNEQVMKSLLYQYAQFHVSEIFPIYSVDVKLEGDTNSKLHEWSYDTNPEIFEQPAYETKNQYRFSDLQYYVKAEGSDLQTYAAADLVTYNQTNAILSIFQTVFVCIVLAVSALMFSKDVSDLVIDPIEAMMQKIEMIAANPLEAVNIEEQEDLIMEELEKQQDVEKLKQKEIEKHMETYVLQRLIMKVGALLAVGFGEAGSEIIAENIKKGGSVDPMIPGKKILAIFGFCDIRNFTDATEVLQQGVMLFVNEIAEIVHSTVDSMSGSANKNIGDAFLLVWKYSPNDYHPDPDNPKNLKLKSDRYIKQKGDMAVIAFLKIITAISISKKLEKYKKHEGLNARMKDYSVKMGFGLHMGWGIEGAIGSGFKIDASYLSPNVNMASRLEAATKQFGSLILVSGILKQYLTEECQKQMRMIDIVTVKGSIVPLELHTIDMSIKNLQSKIKGLRDKFDVSQMTQKESKKFRVVNRFKREDLMKKVQNNQVSVTDLFTTDDELAAAREPYTQVFYETWKQGFEQYIKGSWGDAQQTFQKTLTMIPEHKDGPSNTLLEVIHSCGGKAPKDWKGFRELTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VS71</accession>
    <name>A0A8S1VS71_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription factor CBF/NF-Y/archaeal histone domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0740136</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000074">
        <property type="protein sequence ID" value="CAD8180464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VS71"/>
    <dbReference type="OrthoDB" id="308045at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017054">
        <property type="term" value="C:negative cofactor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042225">
        <property type="entry name" value="Ncb2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46138">
        <property type="entry name" value="PROTEIN DR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46138:SF1">
        <property type="entry name" value="PROTEIN DR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="4">
        <location>
            <begin position="3"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="121"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="121"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="146"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8180464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="155" mass="18208" checksum="7E13CB3AF1F1AF2E" modified="2022-10-12" version="1">MEQLPKATVQQFIKSTTSGDMKCSNEFVTQVMSMAREYIQLVSDQANTICLENGKKTISGEFFYKAIQKLRLEGQIPILKEIEEEIKEEVNVKNQNKARFQDEEHLKQLEEQQKELYEKAKLENSKRQIDEEDHNKPRKALNLQFEQEEGEEDPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VSY6</accession>
    <name>A0A8S1VSY6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Fibrocystin-L</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0700171</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000070">
        <property type="protein sequence ID" value="CAD8178892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="446578at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00102">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00603">
        <property type="entry name" value="IPT_PCSR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052387">
        <property type="entry name" value="Fibrocystin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019316">
        <property type="entry name" value="G8_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46769:SF2">
        <property type="entry name" value="FIBROCYSTIN-L ISOFORM 2 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46769">
        <property type="entry name" value="POLYCYSTIC KIDNEY AND HEPATIC DISEASE 1 (AUTOSOMAL RECESSIVE)-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10162">
        <property type="entry name" value="G8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01225">
        <property type="entry name" value="G8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51484">
        <property type="entry name" value="G8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="2268"/>
            <end position="2289"/>
        </location>
    </feature>
    <feature type="domain" description="PA14" evidence="7">
        <location>
            <begin position="300"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="G8" evidence="6">
        <location>
            <begin position="1685"/>
            <end position="1804"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="2184"/>
            <end position="2221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="2184"/>
            <end position="2220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8178892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2329" mass="260708" checksum="25B0C747163277AD" modified="2022-10-12" version="1">MLDASKDSTNDAVVILAVSTILGKENSIPIGSLMGGTTLYMKVLGLDQTASNNAVYVGKYPCIISDKGVNGLFVNCKTTKSNQNDHNLLNLKIMVKVQDKPDSICTFSYTYCHFSYTPQNTPKLFYVTPRSNYPRMFSYWRAKWVISSNAVEYLEGQFMGSNRCDRFSIQDLYPKQINYQDDKVVCQVSAEIQAGYYDYTIKSQNGYQINSFGLKQKKVYSDRMYNSKVVPIITGLNTNFASPEGQILEIFGFGFSPFAHQNRVKISGQSESIEVITSTPTSIKVKIPKIQKQLENSIYIQGSGLHYTRWNISGLNYNCGSFRQQIITNMAQLDERIKFDGIYPEPDVQSTFGEQYGQYFRGFLKAPFTGVYRFYISSDNCAQFYIQKTEIFKPIRPDNPIAASNWNPYRNYWFESLSYTSAIKSISQPITLEADNFYYIEVYHINEISEGHLTLSMEIESETIKSNSLNSIYQIQTTYTPIKEVIEFTLYNQNENTLLKGNYRLRFTYGIQKTPQIDTFYIYTSSELTASSSANAMKNAIQSSGAPYYITVISTKLDYNGNTLDDSVISFAGYKYTITFDSHRGSQMYRALPKIVQQTLNGGIIGSTVECIQEPADPISGSFQLSMIINGQETLFQVTDNDYDLKFDVSTTIIANNIEKLTGQSPFVWTVGQPQDGQKWFIVFRTHFEGLYDFRVSNNLLVSGNIIENEFILAGCQKQSICDLTLGDQKTPTLQSYSISGSLLSLSLQEGVDLTITTINIDIEYAGADCTNIQIINYASPYTIICNLEQLNGLNIKEAGEKQIPIIHHKDIGFSKIDQSIIGENVDLQISSIQPNSASSDGGTTITINGMGFPKNLNRDFIFQIDNQNIKPLSISNTQLIFIAPKQLSGGIGSISLQFNSKIATSLFTYDESLRIQVTSLQFYQKSPLFKGDMTITGVNFGNIIEDIKVTLIGNKSYNVKVLSITDTQIKVYLRGGMPGNYRIIVTRKNYGDSYANNDDNLFQYIIPINSVTLEDGTSQAKGSEAGGTVIKITGSNFVQGETLVFIGKAVNWICEIDETRFTSEIIYCTVPNFILKNHNQQQLHYKQHLNQLVQIVLIIVNLHMIMNQVVFKGPITQTATTNLIDNTFTYTIPNLPQGYYTTYILTENGYADKIWISIIELSITTIDNAAIGGQILTINGIDFNVNQHPNVKIGSTICTELQVISSVQIKCRMTRQSGTSAIVTLTQLPSENNSATPYTVEFTLSIKSQQNSPVITSINGVVYNINKKANVVQTGNVVLVFSGSMLNGSSVLVILEYMSNKISGVVSELSDTAVTSTFTDIPAGVYSINILIDNNYAYFIDLTQQTLIVNTDIPISNNPTISYVGGAIITFTGKGFDQNTHFTSVLLCGFNCPITQASYTTLSCETPKLLTKSNLLDTQNHLKYYYQLIDSGSSISTFFDLQQSTYYSSSSASNCFIEVDFGKSRVLKLHQLRYLPRIDIQAIILKGAVFQYTTDSILWQTILTVDQTVHTGWNIYVPAVDISDIKAIRLFDSQGVTGSSCQLAEIELKGWVLSNSNNDYIIPTLCDADIIINGYEIDTISGSAIYSSNSVPIVNTVDPQSGRFSEEAVITISGTGFINGQTTVSIDGVSCNIQSVSETQIIGQTGIKDLDFTQLNGIFQVRVNGNLAVNNQQFIYATKWSDINTWGGYEYPGDGDSVIVNAGQTLIVDVQTPKLMQVLVEGTLTFSDDLDTSMDAHYIIIREGKFNIGTDSIAHQHKVQITLHGNEEDVQMPVMGNKVLGCHQCQLTIHGKERIPTWTLLSTTVQAGTTQIKVDDQYQNAKVYLIIRINEMISFLQIKKFKIINIYNTFECPGLINFLIEDQTGHFFGKVSQAIGNNTYIGPNVTYCTRQEAWNGYWCLGRQITAMGFMSVAPDYNKRLYSPIKLDDGKFFNEINSQMEWHWSGAEPKNLRESKFVALIPSNTIVNMSNAGMNPTASEYQLSKRYLSGRPEDYVILKWQFQVPQVIQVSVGTKVILPGMTTKSQHHNLMNMTDQCGANNYFYQNNTIHFVINGQIGCKVKITLKNTLQISTRLEITTEQFFGDKFLEYARAQLGGDPFNYFIIGIKKSSRRFLDETISHQVTVYWTIVDNVEIGSTDSNYSYQYLEDFATKLEFFNPPPYIGTVLEQSTTINILSNLKFPSTEPTSQIQQTPEATNTNPGNTDFNKNNENNQGSINDNQHNDKEIALTQDEPIYQQKNNENNYNNITGTIKKSIKQTETSSNTVEIVVSVICSVVGVSIIIAALIYYKKIKLAKLIANRNQKVDNEFFKVNLTEQQLQLQNHQQQMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VT46</accession>
    <name>A0A8S1VT46_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0720022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000072">
        <property type="protein sequence ID" value="CAD8179403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VT46"/>
    <dbReference type="OrthoDB" id="300500at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF179">
        <property type="entry name" value="OS10G0565000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8179403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="421" mass="47983" checksum="13B9310D513FAD56" modified="2022-10-12" version="1">MGNCQQCTNFTLENKTAEIKTKLTKSKHTLNFANKFTPQNIEGVIKIQAVFKGYITRKQFSLLSTQQKHSSPILNHDILGFETFTNINDSPNQPQSLILKQNLIQDKLQEEEEEKSKRQVEVNQQLCQSNCNKDIQENQSQVSEFSHPSSLKKQFSFDMTSLCFIPKTKNDQLLILSQTQIKLPIIQMVNGAYYEGQWKNGKAYGFGKYIMVDTSSYVGEWVNNKANGFGTFQLIDGEFFMGHWIDNVVEGQGKYTFVDGTFYEGEWKNDLPNGIGVQTYSNGWRYEGSFLNGIKNGQGILRFPDGSIYEGSFERDVPSDGRIYTGEWKNGLKYGKGVFQWPDGSKYDGYYINDEREGYGILYWPNGQKYLGLWKQGLFHGNGQIIKPNGTSIKGKWIKGKRIQSKLNTNVSGKTKTSQID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W3G3</accession>
    <name>A0A8S1W3G3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0820074</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000082">
        <property type="protein sequence ID" value="CAD8183970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W3G3"/>
    <dbReference type="OrthoDB" id="250329at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45667">
        <property type="entry name" value="S-ADENOSYLMETHIONINE MITOCHONDRIAL CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="63"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="5">
        <location>
            <begin position="8"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="5">
        <location>
            <begin position="109"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="5">
        <location>
            <begin position="210"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8183970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="303" mass="35083" checksum="51A90341597433B9" modified="2022-10-12" version="1">MTEKEQQFKPFLIAFYSGLASMVGDMIMFPFDTIGTRIKAHKSEFLGMKQGYNLIVKNEGFKYLFKGFSTTVMGSFIPYGSYFLAYEYMNYYAIKLTKCLEKDGEKSKLNLLIPLITSPLAEAVSVITYIPFDTLRTRMQMNVPEYNYKGIFSGLMEISRKEGWIRLFQASYLYMASVVVYTTFQMWFYELLRYEILRQRDDQTLHNQPLAIHQSIIATMISTALAAAIVNPVDFIITRYQLVDSSQQQLSVKNLVLEAWHQEGKKAFLKGLGTRVFQCSLFSIFYFPVYDHFKSKYGITLAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W472</accession>
    <name>A0A8S1W472_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADH:ubiquinone oxidoreductase intermediate-associated protein 30 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0750071</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000075">
        <property type="protein sequence ID" value="CAD8180846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W472"/>
    <dbReference type="OrthoDB" id="286739at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013857">
        <property type="entry name" value="NADH-UbQ_OxRdtase-assoc_prot30"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08547">
        <property type="entry name" value="CIA30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase intermediate-associated protein 30" evidence="2">
        <location>
            <begin position="31"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8180846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="252" mass="29428" checksum="E643C0F66A71277B" modified="2022-10-12" version="1">MKIFQKAKQLVTPFTLHLKNSPIVVMDTFCEKGNESKFKVFTDQLTGGDSKGVVVFKEEDEAVTFLGQIQIDKKKQLESLPHVGIEYKYSKSVNIAKFTKILFKMRTDGQIYKIRIGVNSSYDFGQHQAYAYIVDQSQMWNTYVIPLKNFEHQGFDKEVQSLELSAEGLQLYQFDIMKESKINEKIAIQIKEFAISQEELTEQEINFKRPIFYKYLQKQDILDTGREKYNQIPQNNNQKSNQNGKSINNWFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W7F6</accession>
    <name>A0A8S1W7F6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">H-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0850011</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W7F6"/>
    <dbReference type="OrthoDB" id="10445461at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019019">
        <property type="entry name" value="H-type_lectin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09458">
        <property type="entry name" value="H_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035842908" description="H-type lectin domain-containing protein" evidence="1">
        <location>
            <begin position="16"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="H-type lectin" evidence="2">
        <location>
            <begin position="44"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8185291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="427" mass="48492" checksum="887C37A87DB84C86" modified="2022-10-12" version="1">MIYLILSIFYLSVQSYITYDSGTYKGLDLYTQSSFVCKNGYSTTKTISFSGTFEVAPQVFIVLELLDMAESNTDFRFQISTITTTNFNIIASCTSSRQVYHIDFNWYAIDDKRIEVFNSFNMDTITSKQFTHENPNAEIAIISVTSIYYGSGAIDFKLFVSEFTTTSITVDINQDSTKAVNLKQLGYQLILTTNDAVSDLGLRSITSSYTSPALNKLTNKWLVLPIVGFNYLNTLMIVQKKTYTTTASTITYSFSKWGSNPLQFAGVTIREIWISYIFTTQYKALQCSFVRISQKYDLEQLTKPKVYLEIPSVNQVYSTVGDFSLIVDKSISPVQIVVHLKCQKDKKVVGQFNKCNNCSGSQKQYVFSRNCNSNINQISFYLRFTPNVQSYQKLKISILDTSCSITQVLYNQIKSEENILNVQILNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W892</accession>
    <name>A0A8S1W892_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Palmitoyltransferase</fullName>
            <ecNumber evidence="8">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0840134</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8185043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W892"/>
    <dbReference type="OrthoDB" id="19174at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161:SF85">
        <property type="entry name" value="PALMITOYLTRANSFERASE HIP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="443"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="474"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="499"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="72"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="105"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="397"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8185043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="572" mass="67495" checksum="F8E69AA30E738427" modified="2022-10-12" version="1">MNDNVLEEYEQLELDSESHDSKLLPQPQQYVQETLEDIIKQQPIFFEQEIQQGNFNVLILHTPENLDELDSTGYALIHHAISFERLDIIRYLLLKGANPLTKSQIQQSCLMMACHFGNQELVRLFIDLGNKINEQDNLQFTPLLYAIKCNQQLIALYLIALQANTDVKDNAGCTIMHWAAYTNDVYMLKYFEGDKSYNQLDSKGKTPLIRAISNYSNDTVEYLLRTYPDIMPNYETDLKTQAIKKIVETIQFQEFIKHHNTKLIQYIIIGIFYLMIGFLVACNFWRLKSDDTYGYCISLISLFLTLISLFYSIFFLSKSTNRQEEGKKISKSLGHPKISYQFHHNNSGEHKHHILEEEINLDDIQDDLENRNSQNLSELLRAVKLYFEKDEQFINFDISRTCSKCKIIQKAQVYHCEHCNKCVKGFQFHSLYLNKCINNTNHLFYVILLLIYFLTFISNVYFTYYTISNNQISITLFFFMPMFSNFISIIELISYYGMIFGIYVISINLIIQIACIFSNLTEHELMNSEQYKYLYELVVIQEFYYMKLRKIGNIFQNIKTQIWNQYNELRIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WAI0</accession>
    <name>A0A8S1WAI0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0880211</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8187228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WAI0"/>
    <dbReference type="OrthoDB" id="304410at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="53"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8187228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="213" mass="25278" checksum="C3EBACBBCF2C5CAC" modified="2022-10-12" version="1">MRCKTYDNYDSFKTLQSPRLHPFRQHKSSKSEDIDMLQFQNMNYSSVHEVSLNNDQNELIKKQEEQIQKLQHQVEVLEKMIGNYTNIQRLYTFVSQVFNEIGLINKENIFEPKLHSLCLIQINEQKQKLKEQNKFISCLKDLVVSCSPPDYFSADQLPNLKQIWRCIKVMLAECIENKRINQEQQQLISQLTKGMMSQSQKQMMQTLTRNKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WEQ9</accession>
    <name>A0A8S1WEQ9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0880173</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8187152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WEQ9"/>
    <dbReference type="OrthoDB" id="290531at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="110"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8187152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="236" mass="28428" checksum="264506A2B0461422" modified="2022-10-12" version="1">MQTYHLLAQLRIEKPTQKSKFLKILQDHQIIDCSKTQIDLAWEHCQTKEYQQVIQDLLSKRVRSISLKPKVQQKLRTLVQTFDKIDELRLINGIYKQKVYRKICYDKEILKQIQELKLDAERKLKDFINETAQDKNFTQVFQQKLIDFQEERNKLNEQQGKEGSQRAKDRLLNRQKMKSARLNESIQFILKYDDNKDRAQTICNKLLDNQIPYYKSQLSEKQQTRIQNIMNTKFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WER5</accession>
    <name>A0A8S1WER5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0900031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8187763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WER5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8187763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="238" mass="27785" checksum="EE315548655D785D" modified="2022-10-12" version="1">MQIKCLYLLANFNVIKFQDDCGEIKFGILKTLKSVNYDQLGKEMEVIYQNDKTLTKLLSNIEKKGGPKIQILYESQQQTHQIIDDNLRGAVQLDYTNNDSIKIDEPKKVTWKKILLTLFFQIVLICNLYISQTWHLVQVILFAFICLRQILVLSKLKIKELEISLELRQILIWNSFVFLPLISLFMLNFIIQSIALIVNTVAQVISLMITLKYYQACYEHIHIVSFYILLLATILCFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WJU1</accession>
    <name>A0A8S1WJU1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fungal lipase-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0910125</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000091">
        <property type="protein sequence ID" value="CAD8188409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WJU1"/>
    <dbReference type="OrthoDB" id="438440at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051218">
        <property type="entry name" value="Sec_MonoDiacylglyc_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45856">
        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45856:SF25">
        <property type="entry name" value="FUNGAL LIPASE-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035939205" description="Fungal lipase-type domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Fungal lipase-type" evidence="2">
        <location>
            <begin position="86"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8188409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="269" mass="30006" checksum="78FCDE7562CAD8D2" modified="2022-10-12" version="1">MRSSVLILVVLAATINCAFVYNEALATEEAALSFAAYCPDTAINTWKVGYVSTNYPNIEKPLVFENNIAGTKGYIAYNPSYNAITVVFRGSSNIQNWLDNIQFDKVNYNTACNCQVHSGFLDAFNSIKPQVDSLFTKYRGLYPKAIIHVTGHSLGAAMATLYTTELAIAGYTVQLSTFGLPRVGDTAYYNYFSSFTKVTHFRVVHEKDAVPHVPPENFGFNHVDREIWYHKSSYTVCQLDEDPNCSDSVLVPSIQDHMSYMGWSSTVDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WM78</accession>
    <name>A0A8S1WM78_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0980119</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000098">
        <property type="protein sequence ID" value="CAD8191174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WM78"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8191174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="57" mass="6930" checksum="5F9A292513F29793" modified="2022-10-12" version="1">MAQSKQKNVNFAQDLRDQTLLKLQKLIMWFSNVIFIQEERKTYGYSKNYRSELDQEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WMF6</accession>
    <name>A0A8S1WMF6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">60S ribosomal protein L44</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0170142</name>
        <name evidence="6" type="ORF">PPENT_87.1.T0750127</name>
        <name evidence="7" type="ORF">PPENT_87.1.T0980126</name>
        <name evidence="8" type="ORF">PPENT_87.1.T1110086</name>
        <name evidence="9" type="ORF">PPENT_87.1.T1250078</name>
    </gene>
    <organism evidence="7 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL42 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000075">
        <property type="protein sequence ID" value="CAD8180955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000098">
        <property type="protein sequence ID" value="CAD8191188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000111">
        <property type="protein sequence ID" value="CAD8196032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000125">
        <property type="protein sequence ID" value="CAD8200682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WMF6"/>
    <dbReference type="OrthoDB" id="303731at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.80:FF:000001">
        <property type="entry name" value="60S ribosomal protein L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000552">
        <property type="entry name" value="Ribosomal_eL44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053708">
        <property type="entry name" value="Ribosomal_LSU_eL42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10369">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L36A/L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00935">
        <property type="entry name" value="Ribosomal_L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01172">
        <property type="entry name" value="RIBOSOMAL_L44E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8147788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8180955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8191188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8196032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8200682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="12555" checksum="81942583B196359B" modified="2022-10-12" version="1">MVNVPKNRKTYCRKCGSHQSCKVSQYKKSKESPFAQGRRRYDMKQAGYGGQTKPIFRKKAKTTKKVALKLECVKCKLKWLKVIKRCKTIVFVDANQLKKQQEAKKNAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WMT5</accession>
    <name>A0A8S1WMT5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0950095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000095">
        <property type="protein sequence ID" value="CAD8190027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WMT5"/>
    <dbReference type="OrthoDB" id="285861at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035934795" evidence="1">
        <location>
            <begin position="21"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8190027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="367" mass="41884" checksum="3341AB86C395F3F4" modified="2022-10-12" version="1">MILLFIIIGIIKSNAPGTLSCITYLSEQLCEEPGYCIWNGTTCQDYTQNQDCYRINEVGACRENGIYSSIGGSGLCEPLIKLENDYKNVCGITNIVDYNYVRYPIITTGFSTHSLAGQTVAQLKMSAPQQNFIYQVLSVNIQIAKNPDLQIILDLYKTYEAELVKVYIHPYQIEKALIQTLQNLRDDTTPLSPVDKQATMTKFWTLVDVYLKRLQIHKKNYQSYNYFLNFLQGSFSRLYLTIKGQGHMITISWSKYKKNGLIQIISYSPKLVGILNALSDIIFVNVLGEDRTSFTDIENMKISYVQESGTLTNIVRKLKFISDKTQIPHQLMTYTINSAICNSNERECEFSFPSPLSNSTFVFYVEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WP05</accession>
    <name>A0A8S1WP05_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0960125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000096">
        <property type="protein sequence ID" value="CAD8190447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="301332at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="932"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1131"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1251"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1500"/>
            <end position="1517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8190447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1562" mass="185190" checksum="96C735E366CA3E63" modified="2022-10-12" version="1">MQKLYLLFWPSYSKAVHSDEVYLNICGSLFSWITFQKPLEIINSYPLNILISIIYTFLHLTMFIVFSFSLYNLQIISKIFTFYISYFQLIILFHTEIHHLNQQYDLAITLTLGILLYINYLIIVYCSRNYFIISQQHMLRRYSFYNYLIVLADFLYFITFPLINDYVSKYSLLLLSFIYFIDAIIMQPYCIKINLIYIGATGALLISIIIRIVFWTESLYVQFYTITLIVPLCSHIFMKIYQNISDNIFQQKLSISLILHLGEIINHYNQTSIQKSIGDYIIKKFIDVETLNSQTILQLLEYHLRNLEKTDDLEEIQLYRILLKHEIQGTYLQSLLETKRFQIQNRKHSLFFKTIGFLISKKYDQQIQDLYSGENKSSQNYHFQIIKIKETDNLFQNCLGLFTNLLEQKMYIWNQLQQGYDKIENTVIDLTKMYKILNKLKDQILSILQSSEMELILKMQTLKRFNVVELRLLSLYYSFLLNDYQQTKIIEIYIEELIRIENSQTNSSFLNLNILNNDLIIISSSILEQTGKMIKVNTEEISRFFGYEENNKKQNCTLINLYMPKFISQDHDKYVQKFINKGNSTLYNKGKQVFCQDYEGFIFPIILSFLHINEKSEDFILTTSLQKLKSNQDYILFDQNGKILGISKYAYSMLINNQTHNSSTQSMQSQIEQSIYECYIQFWIPNIDTLIQNSNFDHDKYQINKLILELSILTNFTQYLYYFKQYQSINPNFSKEASYFEEFLQYFKQQLSNHSFMISIIQFEVQISMHKLPTEQYYYICSMSKQCKDVQSMLSFDQETSTYNQDEIIKQQHIHAQPPIIQIQPKFITEIEKITEVLPQTIRIIEKQLLTKEINQDRKDELILSNIDSQQIMNKGNSSLQFEKQKNDAIIEFDDQKISQSSRQSKSSNSQVQDQIRELQKNKGLCVSIKKIWIITTFLYISIYLLVFVMLSILSSQNQLLQYDIELVRIPDKFNRLYCSFATIGQMDLERSLLGIDYGEYISYRKINHGKVIRNEMESMMINLKNRFSILENEKRLQNLTVRILNQFSYSEEELTMIQFDMIADSYTEQLFQHIIQGLNYSTSNRLSLIDQYYKLQFIKANLINQITSSQNLIDEIIVEIQNSQETTITILYALLSFKLCFVLLCILCLIQFWKKPFSIVQLQVELLSHLSEEQIKLSINSAKQAKQLISHPYKWKRTNYLFECYHKTIFKDQQCILSKIQNNISTKKTKQNLRLLDYQFNHLGIYLKNCMYLILLLSFIISSFFYMKYGIDSTQSEILLTIQYVQFKQDLDSLVILSQLLKTNSILSGRVRELGFLDLNNQLQDPDKYFHTLELELIEKFQIKQDKAQQVNQIIYQDIINSKKISSNDKEILQTLYQDDLCSVMPDQLPFCNFTNNQFQYFPDYPMPRDEDNNREIFRNGINGIFQKLLVILKSYYQLELQGHKNTNQTETEYFLSTPEFKHYILEYFFDINKAVVKFYTTILESTTDILQNDFQNALFYYLIFGISVLIVQGTLQIQNLSKIQELVSASKFAFILMPLECLNENKCLAIIKQIIKQYDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WSE8</accession>
    <name>A0A8S1WSE8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutathione S-transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1020082</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000102">
        <property type="protein sequence ID" value="CAD8192643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WSE8"/>
    <dbReference type="OrthoDB" id="420389at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00299">
        <property type="entry name" value="GST_C_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00570">
        <property type="entry name" value="GST_N_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050983">
        <property type="entry name" value="GST_Omega/HSP26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43968">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43968:SF6">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE OMEGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="2">
        <location>
            <begin position="83"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8192643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="216" mass="25350" checksum="5D27E9C35211B658" modified="2022-10-12" version="1">MSLVLVVSKTCPHCIRVVLTLNHLKIPYELKYIDMENTPDWFIKASPLEKVPILFVGDTILFESLVIIDYINTLVPSSLLPNDNLQRALNRARAEFSQEIIEILWSLFSAKTLETFNLYMKEMRQLFSQLEIWLQQTKFISDNILTLADFAYAPIFTILPALKQFIQFDIFDGFPRLREYVDNLHALPQLQMSLVENYDLLIQKKIQSREPYFLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WSN8</accession>
    <name>A0A8S1WSN8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1030083</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000103">
        <property type="protein sequence ID" value="CAD8193054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WSN8"/>
    <dbReference type="OrthoDB" id="299436at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8193054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="418" mass="49439" checksum="49E9557B697F7E9B" modified="2022-10-12" version="1">MDFQLPKRYGLNKPTFKVMAGQHFAKFDSSLKTLKNLNTFIDLSKPIKELNYIEHRKVRQIMQHLKFQSQSRKFINHQDEQIAVISQQLEMELENLAKEDCPTPQVDLVKLKFPLQLQTKFVHESAATLETHYNLHRIKEKMNHRNITLTESDLLNKITQINFQTARNCDTKEFYHNFNSSRPRGKKEPQLSLDEKYQILKEYLKDRGIEMGRKEQIPNEGYLQFYHEATNRLIKQTKEQQLLKFRKSITLKTAPENQDHSPNKKMSFTKITTVPTTVGTTPALNAITPSNLTITHQSLIKLNVGNNDSPIILSHRHRTEKEKSPEKPYSEKWQDFQNKAEALQSYYLHEGKEFLQTLEDLENTIEKPYLRHFNQIIKVEQEQGNLEEQQKQNKIMRKRKQNLPKKLRMQKIFKSELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WT42</accession>
    <name>A0A8S1WT42_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1000092</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000100">
        <property type="protein sequence ID" value="CAD8191937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WT42"/>
    <dbReference type="OrthoDB" id="306705at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8191937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="82" mass="9480" checksum="7DF69ACF3D292E87" modified="2022-10-12" version="1">MSLHSSFGYDSLRSVIFAKAIKECEKIQNEHYLSETSSDIKSNISSDSEKHLFDKEYIMESAESLMTFNNQQFSLSQFNTQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WVC5</accession>
    <name>A0A8S1WVC5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA topoisomerase I eukaryotic-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1010069</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the type IB topoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000101">
        <property type="protein sequence ID" value="CAD8192295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WVC5"/>
    <dbReference type="OrthoDB" id="47179at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00659">
        <property type="entry name" value="Topo_IB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.10:FF:000011">
        <property type="entry name" value="DNA topoisomerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025834">
        <property type="entry name" value="TopoI_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013500">
        <property type="entry name" value="TopoI_cat_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008336">
        <property type="entry name" value="TopoI_DNA-bd_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013499">
        <property type="entry name" value="TopoI_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051062">
        <property type="entry name" value="Topoisomerase_IB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10290:SF3">
        <property type="entry name" value="DNA TOPOISOMERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10290">
        <property type="entry name" value="DNA TOPOISOMERASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14370">
        <property type="entry name" value="Topo_C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01028">
        <property type="entry name" value="Topoisom_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02919">
        <property type="entry name" value="Topoisom_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00435">
        <property type="entry name" value="TOPEUc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52038">
        <property type="entry name" value="TOPO_IB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="DNA topoisomerase I eukaryotic-type" evidence="3">
        <location>
            <begin position="180"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="520"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="active site" description="O-(3'-phospho-DNA)-tyrosine intermediate" evidence="1">
        <location>
            <position position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8192295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="596" mass="70317" checksum="7DBAEEBB05120F66" modified="2022-10-12" version="1">MGDKKIKIDEYKEIRLGGFQARSHNWWEEANPEESEEEEQQGQEWRYLEHHGVLFPPFYKRHNVGLIYNGKLINLTEEQEELCTYWAQSIGSPYENKEIYRKNFESLMQTKFEGFQLDKADFTKIKEYLEQQRLLRLNKSDEQKKQEKLERDQRELFYGYALVDGCLERVGNYTLEQPTLFKGRGEHPKAGLLKARIFPEELTVNLSTQAPVPQCKLPGHSWGKIVHRNDVTWLFSYVDSSIRKDNHKYVQFAATSRFKQMNDKRKYEKARRLKNCIDQIRENYTKKLQSDSIKERQLGTATYLIDKLALRVGNEKDEDEADTVGCCSLRVEHIKLKEDDVVAFDFLGKDSMRYNNEVKLAHQIWKNLKLFIKGKQPEDNLFSDISVDDLNDFLKKQMEGLTAKVFRTYNASFTLQQQLDLGGTLDGHSIQQKVDFYDNANKQVAILCNHQKTISKQFELTKKKINTKLECMKTYIHELEQHYKKKNPNEYDEEVQLEEGSQKKNSIFIKKFPGQHDKIKQQIQRTQEKYQKELKGLEKKEENKEIALGTSKTNYNDPRISVAFCKVHDVPIERVFSKTLRNKFIWAMATDSNWKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WW12</accession>
    <name>A0A8S1WW12_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Actin related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1020189</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000102">
        <property type="protein sequence ID" value="CAD8192830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WW12"/>
    <dbReference type="OrthoDB" id="421448at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8192830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="387" mass="44782" checksum="74FE0E210A9F5294" modified="2022-10-12" version="1">MFIPYKKNKGYKIPVIFEFGQQNTRVGYAGTDGPEVVLPSDLLMDSEGNMKFIEQNSTYQPVGFDVKNILLGGQIVDFDIWKEYVEHILNNLLFQERHFPFFFVENGNVKKETRNKIIEMLFEEIDVSSVFFHRNTMLAQYIVGKDNVLVVDCGASHTTITPILDGNVIEKGILRSDIGGEYITNKLYEIWKQQGLAFSRFKNYSQLSPSLQMYGDLEIVREIKHQCVKLYDQPIENLKFNPTVDNQAYELPDQTKLLISEEMYKYPEFLYEPTENFEGLDKALETSLEKIDSDYRKELVQNVILIGGASCTVNMVERFQRTINNLKIFGLSSRSKVQTLPKFFDRQHASWLGASIVASSQIYDQWTLNKSDYDEHGSSLIEKKMFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WZ67</accession>
    <name>A0A8S1WZ67_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1040096</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WZ67"/>
    <dbReference type="OrthoDB" id="302948at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="338"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="3">
        <location>
            <begin position="455"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8193459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="835" mass="99542" checksum="F8234C885C84DA8C" modified="2022-10-12" version="1">MIQNSTTTPPNEDSFRKPIIQIMTQINSQQMRVRNPSQESHKLQSDSHQFKITHPKSPTLTQISLQLASDRITSNNQKDLLLINEEPSNKEDSQQLSVIRKSNDYEQLYHKSNFNKSNILHPSLRLLYFSKSNLISKQILTKQQFKIIHDLSSVYIIPLKRQILNQIQQDSIIKQVIYSVYICFLIFYFTILLIELGCNQLTICNYNIFHYLMVIFQLLDCAYQILINKFCIANMFADVITILPFFAYLLDLGEYQKIFYMFYLIRIYKIIDYMQNLNCYINQYDQLFVIQISIQIHFSTLIAQTLVYMFAEQQQDYINYIQNIFAVLFQNPNIIKRYYFIIYLIRTLLLIFYVYKIKQIINYQTIPLDSYINYTPKTLKNIINYQQKNRVVKFDMRSLPLHLQQQMKREKYFKILQSIPIFNQSFSNQTILNICDIIEEDILKPNKVIKQKQCLHFLLEGQIGIVQKCQLSQKEFKLTKISNTFQIFNNIAFFKNQLQGIEFQSIGYSKIASLDQNQFQQLIRQCPQEFQKYRMLIDTLLIENQYHLINIQCFSCFKQHDITECPVVNYKPNKTQVINNYIINKDQNRVIDFRRQVRVKKRKYIAQPKIRIQDPSESQNSEEYDKVESYQKVLPSSNSLQSQNNNNFINQLELSSVPYINYSHSGQTLQSKRMDSDNFISSQGVSSSISSRKIFINEIKEPIGQSKFQKLDYTEDQQGSLFNNNHPLQRVQDSVLQTKKRLNQNRLKMDDVQQLQRYQTDIQSEIKNRDQRKNQTMVTNKIEQLDNYNRRISIENNFLELFEKFNSYDDYFPHYNVDKQINNYQKMQSIKKDQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X1T0</accession>
    <name>A0A8S1X1T0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1080022</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000108">
        <property type="protein sequence ID" value="CAD8194901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="317435at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8194901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="232" mass="27940" checksum="08FFE4F7B70B6DCA" modified="2022-10-12" version="1">MNQNSQFQQNQDFKCKDHNEKPALFWCRQNDCNENRIFCLNCQKQNKHIQHYNEDVFSIHELNQFLIDQSKQPKNLISECQLQLQLTIKSFDKLISGLSYKFCGIENKINQFKNYQTQQVLDSLIKFDEFKNHLKNNILGFLQKFQNILDDLYIKLELHLIQYQITDQQIQLNKIEQQKGFNKSLXKRNVTNKQQLINLRCVCYYQGYFLNQLIDVKYIITRVNKKYLIIQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X3U1</accession>
    <name>A0A8S1X3U1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1100053</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000110">
        <property type="protein sequence ID" value="CAD8195647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X3U1"/>
    <dbReference type="OrthoDB" id="429991at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051291">
        <property type="entry name" value="CIMAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580:SF28">
        <property type="entry name" value="BOREALIN N-TERMINAL DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580">
        <property type="entry name" value="SHIPPO-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8195647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="243" mass="27110" checksum="832AA22EE65A1BA0" modified="2022-10-12" version="1">MDKSFANSSPLNTSKSKQMYTFSKAKRWIDVKDNLCPPIYQLPTTLSKRAAGIGYGKKINLTHETITPAPNFYSLHKNQEHGWTMGLGREDANKYESIFLGLVQNTPGPGTYKFKDSISPVRYTMRQRLTSRKDQDPKPGPGHYNLPSSINSKGIYPLSQYRNSGAIVLVPPRTHSERKVKDNTPGPGSYKQIGNINPLGTYFCSKFGASKCNKFPRAQRSMSDHHNSSPGPGTYKLPSDFGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X4W5</accession>
    <name>A0A8S1X4W5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1100171</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000110">
        <property type="protein sequence ID" value="CAD8195840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X4W5"/>
    <dbReference type="OrthoDB" id="2687620at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8195840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="149" mass="17481" checksum="AAC90EF6AE64D3A8" modified="2022-10-12" version="1">MFSQEIQQHSKDKILKQGYVNFGLSQSQEIKKFLKEINSSDRLTFPAEHLKKVNNQSILCISLFETLLFSLKLTLCNLPRFLRPSIGFMNMDITIIGKMKTSFFNSMKLSIKFHLEFSKFVSHIDLLKIMKCLITHTQRIHLQKCSYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X7I9</accession>
    <name>A0A8S1X7I9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1070171</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000107">
        <property type="protein sequence ID" value="CAD8194806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X7I9"/>
    <dbReference type="OrthoDB" id="301713at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8194806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="437" mass="51372" checksum="889FBE0CF29388B0" modified="2022-10-12" version="1">MCCSSKKPKSEIDKSQISKPPPSVINNQSQVQNQQESQLVQNQNKPMEVKEVNLMVSQIESPRSRQQKLFESKVEGFKEKYAKLQQDSTEVQFNQKPVDQLKAKLLKEQLIDLQFPNNEIKGPMFLDDNISSYKERGAEFANTLREFDGEIRNMRIVHQTPYNSYSFIGHIKDKGLYFIKCWNQVNLTKFQTFLINLGKQQQKGEQYKKYHAFKVDPLKDTDQNSPIGQMYLISTLEDHNLYSFAASTQFNIDQKLQVAHQILTYIDQTDLKSSGYGNSQFQKKIKFISPSNILISTQRNRIDVKISDWQQHLKDFNDMIPDIELKNNNYTSCSDEPDDQRTYLIKLLLLLFFRLNFNQIESFGNLDGTDQLSRFIDIKQINADSNFYKSSNFSRYIQSKINLIAQPNQEKYQKIWEEMKDKQIQELSFYRLEKREN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X7X5</accession>
    <name>A0A8S1X7X5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1140176</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000114">
        <property type="protein sequence ID" value="CAD8197219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X7X5"/>
    <dbReference type="OrthoDB" id="307044at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51996">
        <property type="entry name" value="TR_MART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8197219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="909" mass="105998" checksum="CA249684F981B28D" modified="2022-10-12" version="1">MKQNLQLIWIDSKFNSTHQQRLRTIINDCSFVHSTDEAVYIIHNKKYGDKFVVIVSSSQIYNQEEYLDSNKIVFCEDSNNHLHKFLENNQLLVLVKSDILELEYYVSKNIEFLNAKESLNLSFCKQVKEKMNYYHQIEETSFRFLLINQKLQQNYLLCESELDNEILNLTSEKELKLLKDSVREKEEKKGVVFTIFEKLIYYYTRDELYKKLNYNLAESNYETMKQIMCILFDGFSKQIHDSKPPKKLYRGINFSSLLRKSYLQTLEDLNYCYTHKKSMFWNTLTSASIDFATAEKFSKGDCKIIFEISLSENNPHPFLKLDEYQSEYQNEEEVILFPQFEFHIVEYRNENGIQYFFIKQVDNNFSMALDKNKREQYWLNRINNELKPKLKIINAFYQNRINYIIKNVRNFPPEVGDVRYQLKNNIDDYFYKLVQYLSQFYQNYEPHKEYLYNLLDVCIQDIKFEFIITDDFLLKLSEFIESVSELLINQFIKIIQQIFNLEDFKGVLIKIWSQYYHQQNYSLFNYNLQNLSLRTPSFRTKLQNWRQLQMEEIKTIQVVKYSTTEKMGYELTLNDGNKVFNNVPTFIKKIDGSASFGYSDKWQGQGKSIKTTGLKVGDVQKDIKVDLANGNYNLKNFSKNKVKFIEEHIGQFGQVGGMIGTTLVEVYCGQLNKVSLDLNGVVTIVSFYSPILPMVISGGCLIKETLDQYKNLKSTFKATVQLGKMIIKTARNVTILQSATYFGTLFGSPLGPIGSASGALIGGIIGGAAIGFIDMYGQLAQFKIQVSIGKRNKSIQKNGLLENPGVNPRVEFSKLGKQVKSLILIALSDTRVIWLAINIPKDQKIITENHKYGKISIRYPYIGPYDHESKVIFYSFGVKDENLNENTVIDELNNKTLHLVCFAKKTIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XC82</accession>
    <name>A0A8S1XC82_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1190070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000119">
        <property type="protein sequence ID" value="CAD8198578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XC82"/>
    <dbReference type="OrthoDB" id="312661at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8198578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="306" mass="36151" checksum="513286683BF8E54D" modified="2022-10-12" version="1">MGNWFDCFRSDNQDNSKSSISNLDDYKPKQSDTKSQSHYDNGNLNKSPQSLKVNIKPNDDNITKPYKRKLRKLRKSSSIVDIKNSEQQLDDKHYLVLIGPSGVGKEKLIKQMNLKKIQYYNSRYSLQDLKNLANRQQQNFQEGILDEYVIVATPSLELDDDINIREIFIKEFQEYFANRKYISSFGIVVNFERTDLMKKKVLQVLKYLRKFKDIISIIVVNMELSDNVDEDKKHLRDSFKHMGCHRILFISKEMKQKQILEQIQEIQFIKTHIDLTDTIFESIDIIDQHRIQSEFISQIIQCQIRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XCA0</accession>
    <name>A0A8S1XCA0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1180126</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000118">
        <property type="protein sequence ID" value="CAD8198422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="77931at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="275"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="323"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="433"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="459"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8198422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="574" mass="67202" checksum="9C3089FA5E9AEE1B" modified="2022-10-12" version="1">MNNFXLGEFNVLNGCLTCQSTQGFYSVIYNATKCSIFDKTKFEAITSNNIKLKPGYWRPHQESDLVNDCFKNIESCKGGWAVGDDICKIGHIGGLCEECDKHNTRGDGYYFKNDQFTCLNCSNFSINILSLVLITIWVFLSAFITLTSVQKTNQLFASLKLTQNFAHILFKMNINQESILLKLLLNYIWIFSVIFTFNIQFSFSFIFVNQMSDTSYFLTRNLDCEISQSFETELIYIRVLGMFTLISLQIFIIQLTVNIFIMLTKGKFSSNISSITIIYLYIQNYAALINQLFSILAKREISRIDYVQGDVSLLFQSLNHQAWIYKFALPLSLLIGLILPISLLLFLYKKRNFFDKIQFRRHIGYLFNEYNTNSSFWEWVKLWKKTIIIVILIYFETNIFLKGILIAMCLMIYQIITSSFLPYIYPKLNKLDLSSGQFCSIAIFLAAVQYICEQQGDQILAQILQILIALICFRFSFPYLFDIISAYYKKYKENFLTYLIIILKKLFPQSSLIWKFNDIIDQWRQKNSRIDRNFKQLRKLTISKKRQEKKEQQQIYTTLSLNKVGEAKLKLLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XCQ9</accession>
    <name>A0A8S1XCQ9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Signal peptidase complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1190072</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SPCS3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000119">
        <property type="protein sequence ID" value="CAD8198582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XCQ9"/>
    <dbReference type="OrthoDB" id="282762at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005787">
        <property type="term" value="C:signal peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007653">
        <property type="entry name" value="SPC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12804">
        <property type="entry name" value="MICROSOMAL SIGNAL PEPTIDASE 23 KD SUBUNIT SPC22/23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12804:SF0">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04573">
        <property type="entry name" value="SPC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8198582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="385" mass="44951" checksum="C0C65E4331E0E8F7" modified="2022-10-12" version="1">MYSITARFNQIVFFTSWTLVVLSLLNQGTAYFFKTGKPTVDLKILSADKFHHFKATYYNGGVTDWDQVTFKFSLEADFEPVYNWNLKQLFLYVNVHHKHQEKGFESDCVIYDKIISRPDDPSSWSTSSKLVLKNQRAEYPLKDIHKQLRNATVNFEVWIEVMPYVGYIRREKLGDFEYKMPQYFAQFQQTAQFFNPDIGNNGTLRMPQKIKKREISMPSYYFDDFKGFLKQPIKITSNITQRRNTHTTTTQDSSQMTQSIKQPNKSLKQLTQANAQDYSKIINIISQFNNTNKLKHAQSANSMQYKLIIPLKEKSINMTRDSRTNSRCSVVSAQGERKRSIKVNDPYGLINNKIMSKTNSKQFLKQKAQDKLVDKLFSQRSDSVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XD95</accession>
    <name>A0A8S1XD95_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1200078</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000120">
        <property type="protein sequence ID" value="CAD8198935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XD95"/>
    <dbReference type="OrthoDB" id="63533at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01860">
        <property type="entry name" value="Rab5_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000808">
        <property type="entry name" value="Small GTP-binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="172"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="196"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8198935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="205" mass="23090" checksum="6102C63056DABF58" modified="2022-10-12" version="1">MNREVKVVLLGDSGVGKSSIVLRFCSDIFKVTHESTLGAAFMARTIEVNGINFKFQIWDTAGQEKYKSLTPLYYREAQVALIVYDITHKDSFDVLKSWVNELKAHGPKKIVQVLVGNKNDLIEDEKVSYDEANNYAQQIGASLKLTSCKENKGIQELFVSIAEQILYEEQNKIVEGKKPEKPQNPSVRVTTDDQNKKKKKEGGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XE54</accession>
    <name>A0A8S1XE54_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1210108</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000121">
        <property type="protein sequence ID" value="CAD8199295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XE54"/>
    <dbReference type="OrthoDB" id="304522at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8199295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="182" mass="20966" checksum="530554864A5E7A2F" modified="2022-10-12" version="1">MKKKSHRFINSFEKDLNQLETPPHRSMLANYEILIKTKNYLDNVFYQKLEKSSIHTQTILAASVTDNKSLVLDAERNLRSITIDHLPQDLKIDSQLRSIVEKKKKIKECSQTKLISGETQCSQTTLRKENSSSFFKKGILKNQSSQDEFLGSQKRSVSVHSGKSVRFNLSNDNIRQLMMFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XFR2</accession>
    <name>A0A8S1XFR2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Hyaluronan/mRNA-binding protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1220100</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000122">
        <property type="protein sequence ID" value="CAD8199691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XFR2"/>
    <dbReference type="OrthoDB" id="311729at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006861">
        <property type="entry name" value="HABP4_PAIRBP1-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04774">
        <property type="entry name" value="HABP4_PAI-RBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01233">
        <property type="entry name" value="HABP4_PAI-RBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hyaluronan/mRNA-binding protein" evidence="2">
        <location>
            <begin position="64"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="235"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8199691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="340" mass="40129" checksum="F9C370E27111D1B0" modified="2022-10-12" version="1">MSNNLFNVLNFSDTEEVPEQQQKKSKKNNNNKEQVIPVEQVQKENTKHDNPAPKQKGVPADPHPKDRQSGTGRGKEQRKEGGGRNNWGNYKDDLKEEKYIAKEKKTQDAPKEEQNEQTIQPVQPPAPEKTLADYYQERGTNVEEVLKKTETKSQVVKQIDEEALKKDKLFVMKTREDEKKLQEQKQKKTRQQQTYRSELNTQAAEYLGFTNQVQEPERKNERRGERRQYDKQEPVQEQQQQGETQAQEEKGERKERGDRQERGDRQDRGDRQDRGPRQNKGYKGDRQDRPQHDRSNRDNNTRNQRGGDKQRKGDDRQEKRPQQRQQEQGIQLDDKDFPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XH31</accession>
    <name>A0A8S1XH31_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1250057</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000125">
        <property type="protein sequence ID" value="CAD8200640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XH31"/>
    <dbReference type="OrthoDB" id="1723750at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF39">
        <property type="entry name" value="HISTIDINE PROTEIN METHYLTRANSFERASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8200640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="194" mass="22175" checksum="167C7404B65BAF60" modified="2022-10-12" version="1">MNQCCDIVYQNCNKLDVIQGSYEGGQTLWECTFDLLQYPKQLSGLDVIDMGCGLGLLGIQALKQGAKSVCFQDYNEDTFEKAILQQLKLNNLEENKNYSFVNGDWETLNLQKVDIILASEIIYREEYYSKVEDFIAKHLRQDGKCIMINKSYYFGVGGSVAGFKSYITKLQVVEEEIINTKKGVKKMIMKLQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XHT6</accession>
    <name>A0A8S1XHT6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1240128</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000124">
        <property type="protein sequence ID" value="CAD8200429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XHT6"/>
    <dbReference type="OrthoDB" id="10497474at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8200429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="67" mass="8023" checksum="1B2166E437534514" modified="2022-10-12" version="1">MEQSIYYIQLVYYKSHKISNQKFKETKIFVKDKNQSQEEGHSNILLEFLIQLFLADSIYKLHALVNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XJ59</accession>
    <name>A0A8S1XJ59_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1260046</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000126">
        <property type="protein sequence ID" value="CAD8200899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XJ59"/>
    <dbReference type="OrthoDB" id="287006at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8200899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1021" mass="122442" checksum="238BDCB96A5686BA" modified="2022-10-12" version="1">MKLVKPPFQIREKQWDNRFYLGKLSDHEIKKQFMIKKKSVKRPTSTNKIVVDEKSLAIRLYELQLLWEQFRIPQFHRAYFLQYNEKDLDAILRETADIKLKQSIVQKLMSLIKGRERCLNFLKTETFEEHKFSELLFHLRILTVNVIEQFKKWRDSLNRCVRWRIDEIDYLVQLRGDLDFLKEYYQDYQRDPFLLWLYKLGKYDTVHSNYHQLQKDMMKRIRDCEILLIENDYFDMQTNILSRKEAQLINKYLRESTIQMEDQLKSQELKTILSPNHKQTKTVQIQDNFTLVPQVVRNKEQFNNLLNQIKNDNDIDSSFPIRDNLFELIDYQDSLILALMQHQKIKGICISQSEQSEQYRKVIIEQIRLENLQLFPQFIQQLSNYLHGNEITIKLVHYMQNEKLIDCEYLKVALKQEGFRWKQQTNDSGRNIRYTIYYKKKEYQQQQMNQFKFNFHVHSTKLNQLMQLVYNNIDDSTNVAQKFKHYVDSRKSEHNPTQIKLKPKIYDDRVVLLDVKLKIPSFQTEVRYEYKYYRIIQDPEITNISFMEIDGQIVYALSLLDNDYYILISKDQICHEQIQISQFQPTEEDLSIPMFCYNDEIADVFNHHINLQFEHLMTIKRKVNTEKQSIEIAPDNFYFSIFYDGHVINSFKIKQHHLIKFKPFKSQINISDDIKIDKSKFMLQCLPLIQKNIESEPLKIMIKNHECICALDNYANSRKWIIYTIVGCQENQLKDVLEQLITQLTLIDPNIQEVGIDLYHEMVNGEFITNKNIQKQLTSLGFKWKIQVNEANEHTRFTKYLLKLQRETQVVDNIVIQYFCTQDFADVNVQPLIQQFSIFYQTLTQSQLVVKRNNLIPFSKYYNKDIPPELYTDQMLETRISQILQNCKYGTKNKIDYIIFNNQVITSNKHPELGTIYFIQLFDIVLCFYQLEKELNTEQIANLIQQSTSDENEQKECLGVEFIQYSKAGSNYSMEINFNMEKNITIETQNFKTISKPYFIGIIEPNWYDKHNQMIASMIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XMR8</accession>
    <name>A0A8S1XMR8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ferritin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1300009</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000130">
        <property type="protein sequence ID" value="CAD8202038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XMR8"/>
    <dbReference type="OrthoDB" id="426882at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00657">
        <property type="entry name" value="Ferritin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007402">
        <property type="entry name" value="DUF455"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42782:SF4">
        <property type="entry name" value="DUF455 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42782">
        <property type="entry name" value="SI:CH73-314G15.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04305">
        <property type="entry name" value="DUF455"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8202038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="244" mass="28692" checksum="C24AB065D8D441BD" modified="2022-10-12" version="1">MINFGKVLLRKSQISLDDIRKFMSLPIDSTDEDIPIYSYDENIMSTYDLEQSIKHIPLKDQVIHSIAHIEYNAMKSYIDTLVRFINQVPAQFQKEFKEELGQIAYEEFCHFELITQLCKYGSQPVHNNLQKRMILTMDSLLGRIAVLSIVNEGRGMDTGLNLIQKLDGDNKYEKVIKKIVQEESNHVRIGLKWFELLCKDQNPQENFITLMNNYQIPKNWKINVQKRKQVGFSDEWIKCLQNWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XN33</accession>
    <name>A0A8S1XN33_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PPENT_87.1.T1310034</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDO010000131">
        <property type="protein sequence ID" value="CAD8202431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XN33"/>
    <dbReference type="OrthoDB" id="297477at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031090">
        <property type="term" value="C:organelle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20336">
        <property type="entry name" value="Rcat_RBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000039">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000051">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="361"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="409"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="135"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="139"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAD8202431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="445" mass="51398" checksum="F1DEAFCB293F163F" modified="2022-10-12" version="1">MDHIEQLCQFEQQKPQYQQLVLPIYQQQLFVSHGPKLGKSIPLKCKGTDYVDSHQSGDPNYHQILQNQQSFNGKTVHSQMQSIISQEIKTEGLKVLVTEEEKQKQIIQRRDSGLSWQSEVKLEIKPEEQSKELKDLQECQICYIEKPKESFIAPLICKHEFCSECLSQHLTQNILKGNVLSITCPQSTCTFAFNDEQIKSLVQEKIYEKYKRFYNRQVISQNKNVRWCPKPDCENYVIGNGNDLLFCICGQSICFQCGNQYHKGMDCIQAMDAQYLQARKDNLIFDCPSCKAPIQKKGGCNHMTCYKCKYQFCWLCRGKYSSYHYVIFNIFGCVFPGGQGSNLQPFKNPMMLRVMMIIPKILLTLILISVLLALLPFFLLYLIVAAPYELSRRVCGFRLRRYRLCGKIGFGILYFFLGLLLSPLTVAIAILVSPCFLIFYLLDNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XQC0</accession>
    <name>A0A8S1XQC0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1320102</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000132">
        <property type="protein sequence ID" value="CAD8202898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XQC0"/>
    <dbReference type="OrthoDB" id="302263at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8202898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="235" mass="28054" checksum="50D979443DA4CF6B" modified="2022-10-12" version="1">MQLMQAENDHQQESSNNIKHLRYKSSYLIRNRRKIYDDNDFREVVQNFYNLVSEINKGQESVQPLLVKKITKPPLRKQTQQSKQRNASKNKQPTKELTQSKRDAHFRPDQQAQQYFNSCFSQQIMSHLLPNFDQPYCKQVPFLYDKPKPHLNFKRNQIHIQTAYQIYIKEFGSGFVESQDPTSFARKVMNSNFNCKTQYQEEEKESDKSEKLNKSYEDGKKKPLKELVREFQSDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XSK5</accession>
    <name>A0A8S1XSK5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1350027</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000135">
        <property type="protein sequence ID" value="CAD8203778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XSK5"/>
    <dbReference type="OrthoDB" id="297937at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8203778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="162" mass="18944" checksum="CFD0F1738A7B90EF" modified="2022-10-12" version="1">MKKMTQTRKKRPQKYNVVDDHKRQQIVEKIFQEKMKIKEVAQLFQIPLSTVKAVKSVYLKEGRIQKKAKRNRSKKVITTIIVALIDKNKIPTDVNEIQKYTKVLFGHTNQNGNVTEQCMKQGIDLLMQMLTQIRKNQPSIGKHSAMLSKLLKNYQQQCQSFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XT02</accession>
    <name>A0A8S1XT02_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1360111</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000136">
        <property type="protein sequence ID" value="CAD8204247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XT02"/>
    <dbReference type="OrthoDB" id="317431at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00882">
        <property type="entry name" value="Ras_like_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8204247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="408" mass="48160" checksum="F4B3F9990613D7DF" modified="2022-10-12" version="1">MFKKKQSPKTNGCHQLFTYKRTTQINTIQQTYNYNTNNQPQQAIFISNLMQKPPYNQIIYKKQIVCKQQTHWKPLQQTIQEKNCNYQYFCKYNQIKSSNNVIPYEETTKNVKPNYQNIKNVVPNDQNAQDLITNDGNAKDLITNDGNAKDLITNDENAKDLITNDENAKDLITNDETINQKISNDFNNMTISNQSFNTSTFSSEEFCEETIPLSLIIGQAGNGKTQIMRKIFDEDVRIQLFKPILQEQLRYYFVDTSSFDFDSDYDSREEQIENYKNLLREYPNRVRSILIVVNFERTDLMKKKVININKYFSKFKNLFTLVITKFNLSEDIQRDKEQLKQSFKFLQAQNIIFVGDDTKKQKLLKDLRAKSLPRLEDGYEFNSVDTLFEEEDEEELKKLQNDLLKKFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XXY4</accession>
    <name>A0A8S1XXY4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1440021</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000144">
        <property type="protein sequence ID" value="CAD8206413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XXY4"/>
    <dbReference type="OrthoDB" id="307068at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="339"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="3">
        <location>
            <begin position="431"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="628"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8206413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="866" mass="102177" checksum="B29B1FDE0ECA2623" modified="2022-10-12" version="1">MIQISSPRNDQVSLKTISEKSKKNKLNYPFQPNDISSAFEINSLSFDQSELESNKPQRNTQQQMKVQQQQFSCQPINNTKKYCYNSDIIQQQKKDLVQQYAKTLNSLLLQDNNNTTSIILQQLINKNLSDAQFAKQQDKKKKIQFNENSLYKFIQVLILLLIGITSPTQMVLENTSFIIYGNMFQITLLISLIMLHLFLKSKLKASDKYKTKNFIIYLAQNLCYEIFILLLSIILFLIHILQLKQLEVINIMQIIVCIICLVKNVSLSNHSTQAGLKILLGNFIFFHLLECYKLSLIYKQSLNLQSFFRNYILILVQCNRVQESGDNIGLALLEIVKLIFNYVLFFQIFIYLLNLWNTYFSIDYLLVNKHKQYEKLMRDLQIDSEMSNKILKQSKSYELEDIKYLSLFDQIISNQYFLLQLRRLLFQQSQLFEQFSNQSKDQLIQFMELHLIHPQQIISCDNLYEEQSIYFLIHGTIDIGIQNNQNFNFEKIEELQQGEHFGQLTFFTGQKDYLSYKSNHYALIFKLKRQNMLKCLETNLLDKEILSIIINQVINKQNYSLLKLQCQCCNSTHHILSNCPQLHMFVDKNEIISTYLSPKNQERLKYIRYQNKKKSNILKFKLPKSTLTRSDASRNIQKKQNNNTQNGERKSSAFNFDNNSDIPKEFTSEKILEQQILQHQLNSINSEHVIQEFQNDTQKKSKLFVALEQNIEHNQQMDINIGTLNLDCQDVLINPYQYIITDSKPYLDECESSNMINNLNSYKSRQMSGTNMNSMQHHQFISLTGQVLYILSMYQVTSILNNEFYNQFTNLNIDKWQNYSYFDAEYNLYNVVQKISQQKKDQNLLKQTQDYNMSIRIEKLIIQDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XZZ7</accession>
    <name>A0A8S1XZZ7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1460015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000146">
        <property type="protein sequence ID" value="CAD8207030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XZZ7"/>
    <dbReference type="OrthoDB" id="166212at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011:SF28">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="38"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="201"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8207030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="554" mass="65716" checksum="4AB917FE3D32DAE3" modified="2022-10-12" version="1">MQQFKILQELIRQLKKPFEKRSEDDLYPIERMVRKIEFFQKYFADQNDLNFSYYFEHEYATQNTSIINIDSVGDKFYIILQGQVGIYVKPNVVEDKASSTKLLEMVKLRFRQAVNNNKFKDYQINKIQSQQSDLVLIKILLPGQSFGEMSLIHNRPRDFTAVALQPCHFAFIRRQYFKDVVRIGQEKSSVKEMGFFANLEIFEGWNVNSIYSIYKMLEQRSYRMGDIVYLSGDIVNKIYLIKEGAVDLQLVFVEEREDLEVRTISPYMKQRGKSLHRKIATLSNHQFLGLEDVHSQEQKHRFSCVSSSSDTILFIIPIELYMKKIYTQPTSQLYVQEFIQIQCKYQDLRNSQLQVAINSQRQQQLQIISKMNQYQNADMLQFFQNLQGRFKTLSQESTKKFQKKSIGERSQNLSAHEKYVDRSFFTRAKDRSLLGKVQTQSLYQNQAYRQVINESRIIEKNLPNLPQFLEKHLPGLHHSKSTQVRYRRQSPQKQQQFQFHNVQTSLNEEEQQIVSNILSKGVVYHRKSNLVKRVRSTENYSYRRALRFQDQTKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y2M6</accession>
    <name>A0A8S1Y2M6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Alpha-tubulin N-acetyltransferase</fullName>
            <shortName evidence="1">Alpha-TAT</shortName>
            <shortName evidence="1">TAT</shortName>
            <ecNumber evidence="1">2.3.1.108</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Acetyltransferase mec-17 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1500009</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-lysyl-[alpha-tubulin] + acetyl-CoA = N(6)-acetyl-L-lysyl-[alpha-tubulin] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15277"/>
            <dbReference type="Rhea" id="RHEA-COMP:11278"/>
            <dbReference type="Rhea" id="RHEA-COMP:11279"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.108"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the acetyltransferase ATAT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.108" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000150">
        <property type="protein sequence ID" value="CAD8208191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y2M6"/>
    <dbReference type="OrthoDB" id="447510at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019799">
        <property type="term" value="F:tubulin N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070507">
        <property type="term" value="P:regulation of microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000216">
        <property type="entry name" value="Alpha-tubulin N-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03130">
        <property type="entry name" value="mec17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007965">
        <property type="entry name" value="GNAT_ATAT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05301">
        <property type="entry name" value="Acetyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51730">
        <property type="entry name" value="GNAT_ATAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="106"/>
            <end position="119"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="site" description="Crucial for catalytic activity" evidence="1">
        <location>
            <position position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8208191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="250" mass="29134" checksum="572B8F9B128DD532" modified="2022-10-12" version="1">MQFQFPLQKALQTNQNGISVISAQNSRRNCYLDEVIDKMGEASAIAQQLKQIITTANKFYGSDQRIYMKADGKNCQGLLKVGKKNLFYRDYCGSIKELQPLCVLDFYVHESVQRMGVGKELFEEMLKNEQIKPEKLAYDRPSQKLMGFLNKHYHLNQYVPQNNNFVIFNQYFGQGNQSNVQGKSQKYSRNSQIDQLDIMMQQITKNKMNQQQLPQQKLGYQMNPPWAIDNNTNIYNNNINSQRTNVYKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y6Z4</accession>
    <name>A0A8S1Y6Z4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VIT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1530047</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000153">
        <property type="protein sequence ID" value="CAD8209063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="312927at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013694">
        <property type="entry name" value="VIT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45737">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45737:SF6">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08487">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51468">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VIT" evidence="1">
        <location>
            <begin position="31"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8209063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="324" mass="38090" checksum="77FF1E53CFCE4C5D" modified="2022-10-12" version="1">MITNKLFKPKLESKQFNVSKKVRQTCCFYPQSLIICYDLIRKIPIPLKGVHYFLKVQPGFVIVELKQTYSTQGYNQPIELEYLFSINNNAAVTKMIVELGDIKVYGIVKEKEEAKQQYEDGIKQGKTMAYSEQDEEFPEIKRVKIGALAPNKQLNIIFEYIQPLDVVLNKLWKVEVQPMVDNNYFSLNQQSQRIYNNYFERLNRYLEKLVNIDQFEYNFKQDITVSIDIGSPITYYKCPTHKILSGNAKNEYAKQEIGEDKLKKLYLMLEDXIAYSLNLNLTLTFCIIKHFFNKLYHCQLFPVYKGNLIQQSINFRSTNIQIDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YGW1</accession>
    <name>A0A8S1YGW1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1850011</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000185">
        <property type="protein sequence ID" value="CAD8213746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YGW1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="91" mass="10800" checksum="57EA5D4865BB43AE" modified="2022-10-12" version="1">MGNLQNKYDVLPLQYMQMILQEEEIYECFSGGGLYDQKCNGMKIGKRIELDKGFYNGKQITHIGEYKTGKKFDIWDIWCIKQYIHLRGRII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YIU1</accession>
    <name>A0A8S1YIU1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T2380001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000238">
        <property type="protein sequence ID" value="CAD8214696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YIU1"/>
    <dbReference type="OrthoDB" id="2544694at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8214696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="89" mass="10720" checksum="A9D926C682160C12" modified="2022-10-12" version="1">MNFPVIQLYQRFYIESNFREVQNVGVKVFFLICKFAIIALAYFITFWKWLEITVAIPYFLQIIGSLLIFESSYFYKQDQENMTSPFPYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YL13</accession>
    <name>A0A8S1YL13_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">rRNA methyltransferase 1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T1980009</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000198">
        <property type="protein sequence ID" value="CAD8214168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YL13"/>
    <dbReference type="OrthoDB" id="270651at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016435">
        <property type="term" value="F:rRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18105">
        <property type="entry name" value="SpoU-like_MRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047182">
        <property type="entry name" value="MRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047261">
        <property type="entry name" value="MRM1_MeTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004441">
        <property type="entry name" value="rRNA_MeTrfase_TrmH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013123">
        <property type="entry name" value="SpoU_subst-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00186">
        <property type="entry name" value="rRNA_methyl_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46103">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46103:SF1">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08032">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00967">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="RNA 2-O ribose methyltransferase substrate binding" evidence="10">
        <location>
            <begin position="18"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8214168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="261" mass="29682" checksum="24E9D6269C0E5CE0" modified="2022-10-12" version="1">MYILKLGSRFSSQKSYDYLYGINPVMSALYANKRQFTQLYVNQTQQNDYINPRISNILNRAQSLKLDVQMIPKNKLERYCSNDHHQNVILKCSKLNYCNQLSDESNFVLLDSVQDPQNFGAILRVCFFLGINTVIVEKKGQCPLSPTVSKTSAGALELMNIFETDNLATFVKQRKHEFQVIGSGFESNSIPIEEFQKDQKKQKKIIIFGSESSGIRTELFKQCDTIVNIMSSRHTFPETLVDSLNVSVSAGIILQQVLNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RUH5</accession>
    <name>A0A8S1RUH5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pru domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0010210</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RUH5"/>
    <dbReference type="OrthoDB" id="7777654at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061133">
        <property type="term" value="F:endopeptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044867">
        <property type="entry name" value="DEUBAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006773">
        <property type="entry name" value="Rpn13/ADRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044868">
        <property type="entry name" value="Rpn13/ADRM1_Pru"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032368">
        <property type="entry name" value="RPN13_DEUBAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12225">
        <property type="entry name" value="ADHESION REGULATING MOLECULE 1 110 KDA CELL MEMBRANE GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12225:SF0">
        <property type="entry name" value="PROTEASOMAL UBIQUITIN RECEPTOR ADRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04683">
        <property type="entry name" value="Rpn13_ADRM1_Pru"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16550">
        <property type="entry name" value="RPN13_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51916">
        <property type="entry name" value="DEUBAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51917">
        <property type="entry name" value="PRU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pru" evidence="4">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="DEUBAD" evidence="3">
        <location>
            <begin position="167"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8131636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="276" mass="33115" checksum="8DDA99423C660A2A" modified="2022-10-12" version="1">MNQEVNIPCGRYEFNQETRKVVMQKDKGLLNLILNDENELWLKWYNLDQNRRLDFERVIFKGTTIFEKVKGQHRVYLLKITDEDSKYFFWMQNEDVNQDQMYIDQFKVIVDSQIIDNLIEFEAQIPLNQQQPEFVANQNRQFENQQRQPLININQLQLLELFQQQLYRHTKQDLSLSDILSSEFLFSVIQDQDYFEALKEYLPIDQQNIQYFKDNLISPQFQQALDQLCLALKGRERSSVLQQLELDNSILEKEYDGVIAFIQAIIKLIKEENKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RX77</accession>
    <name>A0A8S1RX77_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphoenolpyruvate carboxykinase (ATP)</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0020542</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="184182at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004612">
        <property type="term" value="F:phosphoenolpyruvate carboxykinase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001272">
        <property type="entry name" value="PEP_carboxykinase_ATP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30031:SF0">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYKINASE (ATP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30031">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYKINASE ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01293">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8133661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="304" mass="34829" checksum="96181EFF435C5DEE" modified="2022-10-12" version="1">MQKQNMKTHELLQKWGLHNTNVFRNPSVPLIYQLSMQSTEQTSTRPDSINSTGALVAYSGKKCGRVPKDKRIIKDQVTEQDVWWGDVNIPLSRESYNEVEKIALEYLGSREKLFIIDGYAGWDSNHRLRIRVFCTRPYHALFMKNMLIKPTEEELMNDFNGDVDFYIFNAGPQIIQKPIDGVTSEGCVAVNLTERKMVILGTQYAGEMKKGVFGVTHYLFPKQGILTLHSSANEGENGDVTLLFGLSGTGKTTLSADXIYSINIFILSQQQKNTMIFMLQFFQVQNLYLNTFLKRLLERIFMKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S6V4</accession>
    <name>A0A8S1S6V4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0040221</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S6V4"/>
    <dbReference type="OrthoDB" id="294932at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8135433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="807" mass="94121" checksum="24D85A5203E6F880" modified="2022-10-12" version="1">MGCIGSTSRNLQQPTFSAILTEIFELVPREESLKEFNKKSEIMKYFNEFDWAKISSQFKLEVKTLIDHIESYKIRLIDSALYVESKIKLSQNPIIIKMCMHILQELILNLDKFIQLKEIPVQNQFLEESKIEISQRSIQIEHLQTLTQVSDNFLKFELAFSNIISQLINDKMTYAMVWWSQFLETLESFSNKKANKQFQSENIQCILTFLEYIIGTKLGYSDQFEWVTKTNEEMSAVQELLDIISDPFVVMSVYRNAFLRIPQERLFVTKSQKSLFNILMKDHKKFASQPIWHKLTETFLERALNQQLYEELFQMLFNNFDEDYWKNPLDIFDITYIVLEVAKEINQLSNTELFIFTKLVDYLDTPNDKNPFESPSAPPLLKKTSEYKNFHVTSNVKNSIIKLLISLFNSIETNQFILFQQTQTIKAVLNNLRSNPESETFNDTQLCLIALLKQISSDTNSVQSFIQQILTFTYNYNLNLINNTAINIDILINLLIILHECMLKIETFNSTIIITEEMIVMLIEIAEFNHQISLKCAQILTSLFLLAGRINSNENNEINSYLIPNSNIIGLSAFFLSNSVLLSYDQPADIRTLINQFFDFIPGRLTNQQIVSIIAYLIDDSSIKQGNNKLAKSNRKVNFDQYKDKELRLCMIIKLISFMDEEVSKEYNELLYSILGAQFQTYKKKGFGAYQDLSSLLQKNTINTEIQEILSKCTSISPIDFEKIKKQFKENLSNQNNQNGIINLDDIQVKEDIERGSILNQSFQAPSHIEVQLDESFEQQLQTIKKQKMKQEKLEHLTTEQRELMEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SBW8</accession>
    <name>A0A8S1SBW8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0050247</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SBW8"/>
    <dbReference type="OrthoDB" id="205639at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001229">
        <property type="entry name" value="Jacalin-like_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45949">
        <property type="entry name" value="SORTING NEXIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45949:SF2">
        <property type="entry name" value="SORTING NEXIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01419">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="5">
        <location>
            <begin position="142"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="430"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8136632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="523" mass="62787" checksum="DBB8E98B93B0CF98" modified="2022-10-12" version="1">MEFIILGIKQGDLKYFDDSIDLQTLFPYKIHTVKIFFTNQGILGIQNYYYSFSSQSVIKCKEHRSSKMFGVNQQKLVLDSSEYIIQLTWYQNEIGINRVEIQTNKQQIQIGQKDGEKKEFKVEQNYQLGAIGGGYKQQLQFLEWQIIPLVEQQTQYQSQLYQLYLSQIESNQKRSKFEYVGKQYRVCDPQIIQQRLTNKFVQFRIVDNTEQEVIRRFQDVFQLRQILQLRWPGVYIPPLMNKSTFEDYSSEHIENIRKAIEYFLIKLSKITYFAQSVEFNVFITKTNKDSNQEMDYVQNKLKEMTQQTNIEQIDLRFKQNFEEFETKESVNEQQRQKCNDFSLLMSKLESIKVNDFQDIKKLFEQHSKNVNYLSKYILPELHLLHLNLQSEVVIQRKKSNSIDRGLQMQIDTMNDVYDYFVTEQREASVMSQCMNYIKDIEQQKNKLEQKIMSQKLKQEELNTAKIQFQSVSSIWSILVKSYANYYIDNYFKERYQLYLLMINRLAQIQLNNLKLRQNFWQSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SDF3</accession>
    <name>A0A8S1SDF3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0050509</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8137112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SDF3"/>
    <dbReference type="OrthoDB" id="306701at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8137112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="302" mass="35107" checksum="EE3D134F7E4FBDD0" modified="2022-10-12" version="1">MSSVQQFCEDLSNIDLKDPNAKEMMMKKITQYDQNFAQINFHPTPSQLSQNYKRDISKIQLPQNGSEMEKQLKTQFIADGSIFGEYQSILRQAHVPAFVVPPPPPPKPEEQAAQIFLRLPYNYLDQNANTKIESNFIQQPQNQQLSQISNFLSTIQNQNQPNQNQNQQHQMQNPSAIQANILNNQQQMDLQMQINQTPNGLDSDFITNIKKTVNILGGIEDTVQIMKQSLQDYELQHQIERKIEKQPIFKNSTLMNPQKNTQSQTNQQQYHPFERYMATEPLQIVQQSQKLRESKLQQNIHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SGG0</accession>
    <name>A0A8S1SGG0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0070413</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SGG0"/>
    <dbReference type="OrthoDB" id="309965at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026306">
        <property type="entry name" value="RSBN1/Dpy-2/CEP530"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13354:SF11">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13354">
        <property type="entry name" value="ROUND SPERMATID BASIC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035919920" evidence="2">
        <location>
            <begin position="18"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="244"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="325"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="494"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="527"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8138988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1097" mass="122921" checksum="A8C0DFD76EB7D16D" modified="2022-10-12" version="1">MKNSYLLLIMAITLVYGLEESLTLMSLEQMNSLDTSQFDCSRPQSEFIQMENSLSQWQDLMEHKDYIDHDIQVLQGIQAKVGLKASHKQVIKSLSQLKLPKTSTKIGLAEVEALKQQCGCLKNSDDNCDALLKLLSYFILSLRNVQSQCQQHPITVIKIKGKIHDLQVIRAGCGGTIDIKGDEGCDTKEQTESTTKPTDVPATDPIVQPTTDPVPDDQKDSEEPVEETITEETVDTTEEEQEDQQPPVATEETTQPEEGEVEADEKQPEEGSPDAEETEPAVAEEQEGSPEETEAEEGVDAPVVEEEADVQPEEEGTDEQTQPAEEGESTQEEVNQPEEITEPEEEIEIQVPEEGSEENVPESAEEGQAANEEEGQPESTEEGQAETAEEGQAESAEEGQDESAEEGQVETAEEGVAEEDEEGVVEAPEETVTSAEEEELQIDEPAEEVDIEVPAEEEACEEEELDQEEESFDEEVAEEVSEEGSEQEGGQVAEEQEEEQSGGEQEGEEQSQQPDAEETESSVGEPLEFEEEQNDEVPAQEETTADEETPADEETTAFEETTDEEEGSVGDEPVPDDQQDEEQEVLPEGCRQVPEDVLDISWLSTEPIAVSDLPVTESQIIGLSFYFRWLSKFPEAVPEGLNQETKFFVAGLGNALKFYVGQGGFHFSSFDENGQETYKNAGHGDIEGQWVLIYFGISNQKVTGFAQIQGQDVETIEFDVNLTLDESYQLVIGGPDGDVKSFNGQIARVFAFNDELYTETSEFTQFITNCYGIPKNIYSGKRLTIPIEKERTFVSPAAFDYDSIFENQKPMLPDEYSASGWFKWEAPNDQNIWHSGFRWTTNNQETNQNFRLLGDRGLSFFVGDEENLQGHVAFCTYSYKTSNGKSKVNDCKMVKYGNKLTEWFYVYFGYSRHLNKAYGYMEFTHEMAQMEFKDVRHYYASKIYFYLGQDKFYPPFNGKVQGFMLNFFDGSYRTSSYDYTFGYFPRPVYEYDDSKELIPSEPVQKKKCPKVVEITVDNASDVLCALSSYLSAVAQGHDPASDPKAETFCFCLVYEEENASPDLLMLAQIFGGRIKQKSSNVNSSIKKLINKQNALLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SHA5</accession>
    <name>A0A8S1SHA5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0060272</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SHA5"/>
    <dbReference type="OrthoDB" id="10467612at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8137754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="401" mass="47554" checksum="2CB5B98EB708CDC1" modified="2022-10-12" version="1">MPPCLQFYAINNLLQLLIRTNIQHQLQIKQISFESLCQIYNRDSTDLFQFQILIIFSKFCQIDKTIAYQIFKLCHIQQKLDKIKQLIDIIHYLQALQVFCKQMPQCYKNMTLNFIKKQFIFIKKPSTRACKIAIQIINDLISIQQLELDQQLLNIWNEIIIIWVDDNLNELEGSMIQIISKFIKKPNFPKEKIKNKVFLDRLFHNLKGDQIFNVIDSLQIYQILIKQFQICIADDEFFQKLMQLCKNSQNPIITSIILKIIIQMLKSKQLSKEYLSKLQQFNIIRLLPEYLNEVMILIEKINHTSQLSGFLKQKQINELTLVNCIMKIVALILKQNILIVKFKTEIAQFERIFLSEKFQTFMNNISVLKIQEKFTLIRGLLQSIISYEDYEGSDNKKIKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJ04</accession>
    <name>A0A8S1SJ04_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Cystatin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0070300</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJ04"/>
    <dbReference type="OrthoDB" id="2016588at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00042">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053128">
        <property type="entry name" value="Cystatin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018073">
        <property type="entry name" value="Prot_inh_cystat_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12319:SF2">
        <property type="entry name" value="CYSTATIN-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12319">
        <property type="entry name" value="CYSTATIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00031">
        <property type="entry name" value="Cystatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16845">
        <property type="entry name" value="SQAPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00287">
        <property type="entry name" value="CYSTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035796381" description="Cystatin domain-containing protein" evidence="1">
        <location>
            <begin position="17"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Cystatin" evidence="3">
        <location>
            <begin position="32"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Cystatin" evidence="2">
        <location>
            <begin position="181"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8138762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="253" mass="28479" checksum="A34B376B67B06213" modified="2022-10-12" version="1">MKVQIALVLLLALVSAQTGGWKKQNTTIFQATNSNIINEAQVAVQNKYQPEEQGYDFEQVISVETQVVAGTNYNIVLQYGNDEGSIQQYDVIIYSAPWNGQDQITKCEQINGSSASFTQNQATSNESQEQPKLQITKPSSLRLKVRRQDKADSHATGTWENINPEVFEQSNENIINQANIVVAQQYNPQQQGYEFDEVLEVQTQMVSGTNYNIKLLYTNGEGSFQTYDVYLYICPWNPSANQYKATLTGTYSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SK53</accession>
    <name>A0A8S1SK53_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0070296</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SK53"/>
    <dbReference type="OrthoDB" id="282513at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="7"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8138754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="416" mass="48806" checksum="F5DA2867B875F1D1" modified="2022-10-12" version="1">MINKICTPIHLSVKKGHDQVITLLFESDSNIQAVDERSWTPLHYASFYQNRNVVHLLNKYDADEDRYYAIRTKLEEKIILLSNIHYQRKQIRHQRTNIVTVLQIMQKNKIIRRLRNSYKDNNDLLIQYFIMFFEFGFFFIVIILGEEIVYDVKSACRCQQILSEGDCNQKCKWNTDKKKCYDRKCYEIEDEEACFIRNCNYIDNQCQNLDSCSDILVKFFCNLSSKCIYDDSTQNCREEIVQEQNCDSFFTKNACSNYVDSNNNYCVWIDNDVDEGYVQQPGHDYGSCKSYPFQTCDSANLVFNPKELCERNRISCMWLNDSCQQKDCLFIGNDEQLCKNSFASIKDGDKTLLCSWNALTQQCENGFDISTLKQEECYSEQTLFQLTFDDSKQKCTKCPGIGMIVMCSLLIQLIFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SK54</accession>
    <name>A0A8S1SK54_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0080512</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8140220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SK54"/>
    <dbReference type="OrthoDB" id="297227at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="3">
        <location>
            <begin position="270"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="3">
        <location>
            <begin position="815"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="156"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="452"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="495"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="201"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="158"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="452"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="495"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8140220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="927" mass="107725" checksum="907BD56DB8BB5D53" modified="2022-10-12" version="1">MEQEIIDWANTFENTKSIDDLKTGVALCQIISKELFQDKRQCELKIRVINCTKQDYQSCIRNLTFALSLLEESQQIQVKHLTGQMLYQNPLKIFQILQQFYRSKSVSSVSSKPPKAPQKQDEPLFYVDENLVQPNQSIQNTSNEEIIANKYKMIKPNSPYQETNNNQQRSQTHQTEYAENSNRSKPQNQSNDSISNLTPIKSNKESYNQKLKHELQVLENQIAKEQQNLLLKNNRENKSKHIQESQSQHEVLNQDQNCNYQQEHQNLINSEQKQQLIEWLKQIRLIKSNAQGLENKLPKICKNGVIFFDLINRLTGRDEVLKGALRNPKSLREIRHNYRRVLEYLKQLERMSYTYLNSEQSLIDGDEGVFWGLMHDIKVYYTNCGNVAITNCSQSVDTSLRQTQKQQQCQKNTQSFQQNSQSKLQQQITLSQKKQLHKQSFADAQLEITIDQPQQSKSVKPSTANRSPVGPYSRIHNMKQEYDSVNRSLNSLMKKSNIGSQSGTPVNQSLQHSRNFSSSKQQEKARSTSISKNCVTREMELYVQTYFKQKALYNNDNLFTDPIRNGNYILQLLEQAVTKNNFKSILEVEFNVDSALQAIKAIVDAGNLPAWIGKINKSSIMQLDKSAMGLYWWLLKQQTKKIEPNTNQFTLPYRQEEIDQLKIVTLHFAREYTSTIQNFNDLIFQCQTGILLCQIENEIFQQKITPIYTKPIGEKQCIANIRKALDYLKGKPIGQRFVWSEKLIYEGDQLIILGLFEDLRRQGDSYFEDGPYLKKSIVQSKGNSKIGNLIQQEPYSLIQKESSFVQPQQVNKKLIFDDPPQFEWLNNFGFKINWNNDVLEEFKDGIIICTIVQKLENIQFKGLQQKPNTNAGCLVNIRKAFNVLKEKIDMPLELVIEQEKLLKGDKKYIIKLLTVLKNIYKNKLKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SKK7</accession>
    <name>A0A8S1SKK7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0080375</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SKK7"/>
    <dbReference type="OrthoDB" id="303043at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8139946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="208" mass="24346" checksum="717DCBC94E412250" modified="2022-10-12" version="1">MKINSTGSEQRKNDQTLKNTKMEGLKLSQKNQTFKADEEIFKKKEKRNRKFFLKRSISLLQICKQSGQIEFKIGSDFLHIEIMQGGHYPLNQYNLKDIRSEIYLNDSEFQQSNITYSLNNASQSILKSCRNSLSEQNCKSLTQIQQQLKEINAFCQNIQKQNNKICQVDPQQQDSILFKQNDQILQKIKETQQILLVQHQAIKNILQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SPZ9</accession>
    <name>A0A8S1SPZ9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0100036</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SPZ9"/>
    <dbReference type="OrthoDB" id="295152at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="189"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="49"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="199"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8141287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="295" mass="34462" checksum="ECC97EF5EFA0D597" modified="2022-10-12" version="1">MSINEQLLQMMTDICSTLRQEPAVTKEQSTQEILNALKLHIFILLELVSQNKSLEYDELEKAVQKAEAEIRSHVRLEQQMKLYTDNLQEKIEQLEQEKQQIEQDRKASTLQLNFLSSKEIEKKPSNLSQQREGSPNIIKKSYIMGNQIYHRQHQSNLITYQDQQIPNKNTSQEPINRERRSANTQHISYIPGKGLPLKNNVSHQNNSIHNPPSNRKDDRNKSQQSQERIIDKSQSLLKSQNSYSYIKMYNIQKLIINKNQNDNIKQATQLTQQTKSKTIHGVCNNILDESHYKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SSM6</accession>
    <name>A0A8S1SSM6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA mismatch repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0130290</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the post-replicative DNA mismatch repair system (MMR).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SSM6"/>
    <dbReference type="OrthoDB" id="10252754at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032301">
        <property type="term" value="C:MutSalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1420.10:FF:000005">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007861">
        <property type="entry name" value="DNA_mismatch_repair_MutS_clamp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF148">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05190">
        <property type="entry name" value="MutS_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="7">
        <location>
            <begin position="972"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="68"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="125"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="782"/>
            <end position="809"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR037677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8144571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1105" mass="128702" checksum="3045FA072C26E78E" modified="2022-10-12" version="1">MKQTTLQRFLTSSQKKNDTTNLNVNKTSNSNTIVEEKKIDVQYIKPCTSITKEEVQAIKDKLIEKAQEKPHQFSRLKRNNFVDSDDEQQQKYEDQNQFKIDKFSFVCSQEKNKEKKSLAVFIKQDNTKNNTKSQNKQKKSEYDKQGKSLKQPTKQEEEEFDNSQENMQGNLDFKLPTDKINVYEEFDDVTPKWASIGSSRDKSGKLHGTQDADPTTLLIPQNEFNKLTKCMQQFWKYKSENFDKIIFFKLGKFYELFYEDAYIGNKYLDLNWMGRKMHTGFPEKAVHKYKALLLEYGYKVVIVDQTETPEQMNQRVAQNKKIGTGNTDKIVQRQISEILTKGTYLYEEGESQMNLDEKVLLVIRKKILSNTIEEYGIAIFERQTNTISLAFIENRDKNYESLKTLLLHMRPVETVIDQHNLPQIDPITKMISGSVIKSVISHLTASKDNWDEKKALFRLEQYYQNGFPTAIKFYQNNQVVLQALNGLFTYLNQILILDRVLGCAKFKLYDEEFSLQQCMILDSQAIYHLEILQTTTFVDKKDYSLFGVINKTVTPGGHRLLRRWVCAPLYQVDQIRERQTMVSDISQFRKERDLFRQSIKSFPDFERRCSRIYEYSIKTESKAVFYENLSEQRLKEFKNLTKALRLAQQEITLFGQYKINFRSEKLRKMMTFETDGGLLPNVQEQLDEFEQYIIWEKEKDKEVPKPVTGVLESYDQSVAEVELVQQKLYDYLQQIKRKLFKNNSNLQYVHTKFRYEIEIPDELVQKSKPEDFEFTSSRQGYKRYITQEIKDLVTELEQAEETKKQQLTAFGNFIFKHFQSNQYVWDSLIKILNELDALCSLSVFGDTSEGKMTMPKFTTEKIKLVIKEGKHPCLTNINFVSNSIDMGEKLCKFQLLTGPNMGGKSTTLRMVCVLAILAQLGCSVPCDEMELSPIDRIFTRIGAKDYLMEGKSTFYVELEETLIPLKYGTKNSLFITDELGRGTSTYDGVAIASAVMNYLIKTIQCRVLFATHFRILIEEAKLINEVTNVHMACYIQDGKVIFLYRLKEGACEASFGINVAKVVGIDNSIIKKAEEKANFFENKVQKNTEQTLQKFNQIINEYGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ST28</accession>
    <name>A0A8S1ST28_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0100239</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ST28"/>
    <dbReference type="OrthoDB" id="10255522at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000794">
        <property type="term" value="C:condensed nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030870">
        <property type="term" value="C:Mre11 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003691">
        <property type="term" value="F:double-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051880">
        <property type="term" value="F:G-quadruplex DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043047">
        <property type="term" value="F:single-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070192">
        <property type="term" value="P:chromosome organization involved in meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000722">
        <property type="term" value="P:telomere maintenance via recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007004">
        <property type="term" value="P:telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18867:SF12">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18867">
        <property type="entry name" value="RAD50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="119"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="232"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="378"/>
            <end position="1022"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8141654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1075" mass="127045" checksum="858DC0A0DDCD7E19" modified="2022-10-12" version="1">MAYFRPQSSQVISVRSPSRIIPMASNMGNSYVIPAAQNLPTTIIVKEKNPVSYDREKENDDEQKDELELWKKKYFTLEAQLAKYDIENEIKDELQSRILGSQEDVKKFQIDMSKIQADMDKQRSDMNDLVIENQKLLNQQVEVERLKKLVSEREDTIGQLSQKCREYQEQLQELEAFQQALQDMHNQNEGKEKMIEQWKLKYMENDQKKQKEIDELKLKLAEYNPTEIQCLKTQFDRERQEWQNNQKKLQQDLADALKQLDQWKIKYNNLDMQHQQLSSAQKESVARIDALNQEQNKLTVQVHTLNDDKQKLQIELEATKGQLKLHQGLIGELDRLKRLLTDKSAELQQLQQQSQQKDIDLNNADLTISEQNQKIKSLSLLQQENQRLSQELDQQHRLNEQLHQENADLLKNLDRFKLLEEEKMQLENKVSMLAAEIERLKVQLKQKNDKILQEQEELKRLQEQLNEIDELQKQIEDLIRQLEQANNTIDELEKKLQAISELEQKLHDANNKIYDLENKVAMLSAESQRLRYLNDQKTEQLKSAEQQLSDLNLLKEQLNQLQNKYDAQQQMNQNYQAELEKLRGQSTQADTNIAELRRQLDEQKAQNIVHKQANSEQVIADLQQQLSSLQQSYKKVSESNLANEEDPTLDLQNRITLLKQENQRLNQTIVQKNQEVLNHQQQNQQLQQDLLTQDTLKVQIQQLHEQNSLLQEQIQNEKQGRQGLEQRIQQSEQEKYDLQSKCAMLSTHIEGMKYKLEKMENVDELKKIIQDQQAQLAEMDVLKLEIESLNERIAELEKELKLWKQKYESLDQQYQQLLHIKEQMENKLAMLSSEIERLKVLNQKKQGEIDQQNQELMKMDQELNDLHNQLEDINELKTQLNSLENQLQQQIDENQNKSNEITQLKQQVAEIEGLLVNQEDLQNQIKKLEKESQSKDEIIEQFKQKITQLESKIAELEDIKYKYEDKMALLSSEVKRYEFKAKKLEDKSNELQTQVDQLTHDLNEADQKIVDLEKDLGQWKFEYSKLNSLQYKIDEYSFLLVVSFAEIEALRSQNFSLNEQLDNHQKTKVALALAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SVL0</accession>
    <name>A0A8S1SVL0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0120375</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="318111at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2392"/>
            <end position="2411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2432"/>
            <end position="2453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2503"/>
            <end position="2524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2536"/>
            <end position="2561"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2588"/>
            <end position="2615"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2652"/>
            <end position="2678"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2729"/>
            <end position="2753"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8143728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2872" mass="334997" checksum="1F77CBB2643DDDCA" modified="2022-10-12" version="1">MYDYTGGLNVIEIDNNFLNFNIFPGKISKVFQTDINFFDNLNSFLENCNAVFICNTETIEILTSIEYFNGDHIVDGVSQYEILRNQYKISGWVKQDKFGYSIIKNQILFRLSINQDYSDHNLIGNKLLYVIYIQNENQDLIGIEIHTYSYDMPIKSKTYTTSPGVIQKVLDKKYGELLNKWHFFQFEVGSNLNQGQPIYRIYFPSEDTHTQIVWDQEIHYFKNTMLYYHLGGDDIYYHNFQGYLSNWKLQTFCFEFDSTLDIPCDYTCLTCKGPTNFDCLSCHINSHRLYSDLGYSCLCEYGFYEIPGNPVCQNYLEEIQTLQLIYQENECYLEGYFNCDEQNIQCEYGYFQYNKQCIKCPLNENYKFLMDCSNCLIYPDQFASTLLCEEKYLSNSESEIFIRDWKYIDEKQLYQMHITADNKHYLQIAAGLTNYKCKSGLYMDENNNCQSCIENCQFCNGPSTYLCTQCYMGYKKSLFGNCVECENCRLCSTENNEEICLLAAFCKPLQYWKDKKCYNCGLYCQTCNQDQCFTCFNETKYYVRLDRQSCGICSIENCKYCFEYLYLGGYYYTSFESSIYLGFYDQYQTFIGCAECYPGYAFSLITNKCEIQSEDTKDCDSGVYDYQDTFICLKSTLLTTSIQNNDCSDMLNCYQCIKQYTYQIMNFCVVCQDGYYSEWLTGICQPCPESCKVCVQQHGDRNDYWKSEIKAFYLFVLNPSKEYNDFEYYGIIKEQLQVLCLQCYNNYLLHKNICYSGCSEDCLDCRIIEQNQEPKILAKCFKCRANQYGPVRSLVSGQLNQCQMCAPYCSLCIPRSTEEIKKINGYFNMDSPYNYFTNKCLLKSESQNEIINILPTTLNVINCKNLNKCNKSIVSRYNVYCSNYYTDPNDLEKKQTLSFINTFIMGELLTYEVSQLYLYLNEQTIQEFTYDLEMKCQQPCIQEAGFYSVIQTNLQKNVFHLAKVNLHVYSKSNQCAFLQSRPFYINNYKSVLFKNILFQSVLYAISYEQKVVNVLFQINNQFDTIMITFENCSFTQKKIAIPNYLTITSNRPQHTKLLNLNLYNLQVQQSKIFNLQPTDDTIISSLIVHNFKLSNSEFKNTTLFYFNALTYNLKYSILFENVSIIDTNFTQNSLFINLEFEKSYQIEHVELRNFYLENVIFKDQSTFINLLSCKNLSLTNFTLKNILMNDNSAFLLANNFTINEISLENTFLNSSYFISNQQSNTYLINQYKNTKTFEIYGIRFINNKYSNEKSFIYLIRQFETENIVFKIQNLLFSNNNYYKLDSGTQYFYFTSYKQALIYIECSSCQFLDIEIKRGVGNPEIAIMKSTNLLIQNFNVEQYDQLHNKDRFLHSSFNCISQYNYLDLYFVLYIGQFHKVHLMEILINKLIVKDYPLILFEGQSLTEQVLSGIIMIQNSYFKDNFLITTKLNVYSTIISIKTQQQTYIKFEDVNFINNWLNKYEDEVQLHSSTTILVETFQGEIILSNTNFTSNILTNSSDSVMFIKTQNLIFQDCHFINQNKLNIDLIRNNILLDLNQNNLELPFDKIFQILSLGGNGNLFVNSLKFLDSSINNSIALQGGGFYIATTSQGEIQIINSQFFSTQTILNGYSFSLGGLLYLDAQNSRLDFKLENSSIFRSYSRTEGGAIFINPSRIENQITINNVSFISSFSFKYSIMTYSPNQQSSLQSKIIFNNSRFQNNQSEFNQYIKLVEDLSTQEIYPYQIDNPSIHIYYCDFTLLNSIFSQIHYANLMQIENGRDILIENVTVSDCQVFNSPLIKISLLNNLPGQLVMRKINIQQITQYINSDILPCKNNQKEKINKLVCPIDNNKIIYKPEIEFQQDDLTYQLNCNLNMLYSEISNKFALVELKDFTQQHQINIFNFDISLNLCDNCYYGLITIQQIEFNSVNLKINEIIMQNNTCGIIGCLSILNNFDESVLDFSESSRLLSERNINLLENYVDFNISAKITNSLFKNNNASYGGGSFFANLNIYIDNCIFLNNLAQKSGGAIYFFSTQNKLLLTNTEILNNSAQIAGGIYVSDEYINQHKDFNLLLKDNIGKQFANNIFEYPIQLLFSQKQNIIQNNQILLKNTTTIIDQVLIQPYSYYGSIEQSKVLLYPSGIPIAEYQYFDSSTNQYIPYNLHFRIIATNSINEKMSNLSESTCQIFASKIDTSIVQDYNNLQLDEKETSRYLSNNKQQFNTDTGDFNFDNLIIYFNPIEEDQYNLQLAIRCKIVQIPQYSEEFPYGITSVIKNYLLLLKLRTFKCQLGEYLNIISGGCVQCDISQNQYSVSFNASKCDTKDEIKMKNIQPAMIELRPGYWRAFYYSNYIEECYHLHLNCLGGWFAGDSSCALGHIGALCEQCDLYDLTGNGQYSQSNKYACYSCEDMAGNLVNIAIVICWTLLSVFLSVNSTLKMIEDLIKYSRLRTLGKFYLFESASTSVLIKVFTNYLQIISSIATFQLSIPNGITLAFTSVGNPIESMAFSLDCYLVQASEVPIHYLRLVWGFFMIGIYIFTYLFIHLILQVFINKQQLTITFIVTTLIYIYIYLSPNLFGGLISLLSYRSISNIKWISANVAYQYETSLHYKWLIIFVTPAFLVIGVILPLFFWFIVYYYRKRLDEPKIRKVWGYLYNEYKLSSYYWETIKIMSKEFIILVLIYYEDYIPIKALLIFFILHIYGKLTNQYKPYQIGDLNNIDSLQTSICKISILLASLIFTAQNLSISEVIFPSYTILAVINFAFTLKIIYKIFFAYFEKLQVQIDSIKIYLSYRLPSFIKKNKFCAILLKNTSERKQIIRQRFLMIKAYLFAEARSLIQYKESQKQTSKTSRRKYDVGTYDNYSASPQNKNKIVRLNTNYNSSRFTLNIKQSSSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SX84</accession>
    <name>A0A8S1SX84_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0120480</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SX84"/>
    <dbReference type="OrthoDB" id="301970at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8143939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="110" mass="12521" checksum="0D5E214D4F60F597" modified="2022-10-12" version="1">MISGYLQDKLIAQSTIQEVNGTDMLATLERRTTKIYMGGQSQQQLNPLDLVQISSALPQFSLQQNIDPRLQNLLNSNYQQQISQLASYPNIDLINQVKEQAKVVRMHYQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T182</accession>
    <name>A0A8S1T182_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0140248</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T182"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000683">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="130"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="480"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="487"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="510"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8145448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="544" mass="62867" checksum="8FD99437737A3927" modified="2022-10-12" version="1">MLQQSQQIDYLYIPTSKNLQQLLKTTTFWTIGNIQNTHLIKSQLLNRFKNKNYEFALTNNGYFIKFGTKNIKYCNLLNNLYIITYTDKYFTIRSQDRSKEYQGPNEKQTRIWIEYLTRFCIIRGEFYNKFKFINQISSGNYSKGYLIQDNKNRQYVCKSFEKADLQMVIKLRDSVIGEILLLRQVNHPNIIKLYEIHEDSKNIYMIYEWARGELFQEFQKIKAKKSRFTEQQVSQIMRQIFLGLSYIHSLNIIHRDIKLENILLKDQNQIVIADFGLAAKKLPKFEFKKYGTPGYIAPEVLNLKVYNEKIDIFSAGVVAYILLTQKPVFTGSTISAILNQNTAGKIDFERPSFINLSKDAQFFLRKVLSLGEDQRPSASDCLESSFLKNQFQLNEIGTPIKPNLDIRLSYLSSSILPQQINLSKAKRKSMRQYILLKQAIITEKQKQQMLPQIAEQQNGEEEDNERLIVQESVKNIAGSFYTENQKGSQNSDDSNGDSSGESMNGSSSSESEDDSNLFLEEDDLQNLSSKIAGLGGGLQIKIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T1I6</accession>
    <name>A0A8S1T1I6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0140197</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T1I6"/>
    <dbReference type="OrthoDB" id="312660at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14003">
        <property type="entry name" value="STKc_AMPK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000592">
        <property type="entry name" value="CAMK family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF82">
        <property type="entry name" value="KP78A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="13"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="12">
        <location>
            <begin position="278"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="465"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="467"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8145347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="560" mass="65571" checksum="57705209953FA1C0" modified="2022-10-12" version="1">MSNNEQTKQVQNYVLDRTLGKGTFGKVKLGYHTICDEYVAVKILEKRKIESDADFVRVQREIAILRKVEHQNVIKLYEILESDTNLYLVMEYAKGGELFDYIVKKNQLSEPAATKYFIQLIDAVEYLHSQKIVHRDLKPENLLLDEQRNLKVADFGLSNIYKDNDQLKTACGSPCYAAPEMLYGKLYGGQKSDIWSCGIILYAMLCGYLPFEHENTKKLYEMIKYEDYEKPKNISPIAQDLLKQLLTKDPSLRIGFNEIKQHPFYKKMIIQPQQGLISKDQVVLKKLSELGYDVNSVIDQVQKKKHNSNTASYWLLMKKYSSNQQPIAKQNTQLLQQTQQQIKKNQILQSQLQQIYQPMEYRQLQSSTGGSNSREKFQQQRQLRYSLPHNKNRIDYSQFQDVHTGIQNKLNNIQMQGKQTPSNIIDYVRQKTHSVQEKAHCHTPEKVDLVIRQPGKKIQLLMQRKKNPTEQMEKSEQTDNGLKEKIKDLTKRSRNKTQPLKKINDKMERYANNYTHQLIKQNSPQDSFIVKFYKPSIRVRHSSNQENSLKQNRLLTHNFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TAX2</accession>
    <name>A0A8S1TAX2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0170149</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TAX2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035920098" evidence="1">
        <location>
            <begin position="16"/>
            <end position="1238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8147802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1238" mass="146267" checksum="2FD815BC2BC9CBF3" modified="2022-10-12" version="1">MFLFILLCITNFGLSQQQNNLICYYESDDSALVLDIQRPQLQLIEIQADSSNGDTRGFGFWSKYIANLIPKEESFFYRPFDDPNCQTEVCQFNGFYFLKLLDDDEFNFAAIILNMNDNPVSLTHDFYLFKKNSDEIQQSSVNFEANNYECIWFYTSILYSSVDQKIRFYTNYNNQLFAFDYKLMSQKITIILGGYDTDISQNFVLSWNSLLFFKGYFSLIQEYVPFFYDDNFFNNLLSNCPYQKQKTLQISEDLFRSMDPNQNINDLEVLEYLYLTKNQRYTVKGWIKQNYIEAYNYYLKEYGLTQNVMDQAVISISQQHYIFNRKTGDRMIDFYYTVDFENNNETVITFRTEFYRIPLQVPLYEDEELRKFDALKIKKDYFYEKTQQWHYLVIDKGRNPVNGATLQLRLYFVNEEPLVYNFGTQNYNSQFSGSIISISFKVSAYSLSLKSRCKLEKFFFMSGHIEDNTDRYDCDSSCKDCNGPTKYHCISCYTELNQFLTQFHSCECLYLNEYNQKSGICEPIERSQSLSIMKGEYTDQSCQFGYFQVQFNNQYFCLQCPQKQEKHILCGDCFYKPITWYLKPICTFDYLQEQNTYAYIKSQRSNIQIDIYYINNEMKLQLVEGASEFCESTQLGCQFSSEFHLGVQIRMKCKTNRYYDNFQCLNCIESCIVCQSKDVCLTCIENHYFNYQKKMCLSCPKECTICQNAPTSDFGYQCLTCQEKHTVNHLGQCKPCGIYCEFCKEDFNIQTQEYFIRCLKCIDNKIMSIRFNGVDCIIIDIPNCQQAFITSKTKLQYNALTYNFQPSNDLSDEQPICALCQWNYLYFFDQHTCMEMDQINCQIGLTKRFIYNGIDYGVQGVCLISDSTSSFSIQGEICNNEIPNCYWCYTKKIKTNAYCLKCHQGYYTSRLSGQCLLCPDNLHCKTCYHSTIGYNDEWKNNIIVLNYYLQTQDNNSYFYWNSDQSQNLDDYQIICTSCYSGYVLKDNKCIKYCDDSCKSCIYLNDKYVCQTCGQNIYHNLLSLVQYQCSVCPSYCELCRERTDDEILSVNSLFIKTISNSIYTYQCLKPYENDETLFYDSTIGQFISCQNSEQCENVLTFELNLFCQDEDYFDQLQLIQDTDLKNQFKKQNIMFSQLLQSNIQGNSFQIFDIDSIYEIMNKKTIKRIKLILQSNIDQVCSIPQLSYITQAFSQNVFSAMQYIYIQLKIVIFNQHLNLILKKNCKQQFLKNYTLQILQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TB04</accession>
    <name>A0A8S1TB04_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0180356</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8149008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TB04"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="RCC1" evidence="1">
        <location>
            <begin position="11"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="77"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8149008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="125" mass="14406" checksum="53DB62EE0A841D0B" modified="2022-10-12" version="1">MHFNFAISQQSQIFSWGNGQSFVLGTRNENSQFKPKDITSIFKNEKLFQISLGASHVIAYTSTDLNYNTQKVDESIIKIQDQNTKNKKPRKSNSRDMSKERSLSMKRSVQKFDESVIPVKDLKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TGB5</accession>
    <name>A0A8S1TGB5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPENT_87.1.T0220367</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8152275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TGB5"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000315">
        <property type="entry name" value="Calcium-dependent protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="123"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8152275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="479" mass="55671" checksum="3625FD3FF7BFF4AB" modified="2022-10-12" version="1">MDVINKDSFFDDPDTSVFWNVTEYPTKDAVLDLHDTMYIDQDSELHKGTLGISNHYLLYFKKQTIKKWINLDNATIEYIKHQKVGLGIKITKNKQYFEFFGDVEPWYNYLKQFCIQRNFSHHYTLLKKIGQGNFADVFKAVNKNDGSEYAIKCFRKAKLNESVDRLAIIKEISIMKKIQHESIIHLYEVFEGDEFLFLVLEYLKGGELHQYMKKSPPFSEEKCSKLIFRLLKAVSSIHEKGILHRDIKPENIMLRNKEDVENICIGDFGLADYYSQSGQYLFTRCGTPGYVAPELLQDKIYDFKIDIYSIGILMFILIAGKSPFDGKDYDDVVMRNYYAKVKFEDCKLSDIGMDLLQGLMNKNPIKRLTAEEALSHPWFASENLTKICQFKIRKQNQTTQKKLAYFDTNIKQYSAILTQFSPKSSVSNLSPYCVTKCNIEDSPQTPNTPVILKPINTPRTSGGYKLRQVRRFQIPLQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TNF6</accession>
    <name>A0A8S1TNF6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">P-loop containing nucleoside triphosphate hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0230258</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="392140at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031380">
        <property type="term" value="C:nuclear RNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031048">
        <property type="term" value="P:regulatory ncRNA-mediated heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06008">
        <property type="entry name" value="NF-X1-zinc-finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000967">
        <property type="entry name" value="Znf_NFX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057373">
        <property type="entry name" value="ZNFX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF341">
        <property type="entry name" value="NFX1-TYPE ZINC FINGER-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25396">
        <property type="entry name" value="ZNFX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00438">
        <property type="entry name" value="ZnF_NFX"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="472"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="7">
        <location>
            <begin position="1517"/>
            <end position="1542"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="7">
        <location>
            <begin position="1546"/>
            <end position="1562"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="7">
        <location>
            <begin position="1625"/>
            <end position="1646"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="7">
        <location>
            <begin position="1876"/>
            <end position="1896"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="7">
        <location>
            <begin position="1904"/>
            <end position="1927"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="7">
        <location>
            <begin position="2042"/>
            <end position="2063"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="7">
        <location>
            <begin position="2213"/>
            <end position="2230"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="7">
        <location>
            <begin position="2266"/>
            <end position="2290"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="833"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="1202"/>
            <end position="1229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8152822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2296" mass="270821" checksum="9E64082FC8144896" modified="2022-10-12" version="1">MYYYEEKRKQDEIYTPKYNQKDQYYQQSKCYQSNQQPQKVQRNTQQREQRIIEQKKIERKNEKQIQKQKKEQFNLFFQTISDKDQNKVIQELYNQNNLKDLFFNLRICANSYDSYTSLIKNLREAYQQNFNVILSCSNFNTIIFQRCTLLQLYQNSIEDLDQKLIDLYHLFFTMKDLDYQALKQSSIASKYITPSFKVKQVQYVINLINFQIKYTPPTNFQNALEFFPNSGMHSQLFGKLEILSKENTNVHRYIDYHFNLLREDYVLEFKEAIISLNELGFENIDKSKTSQIKLYHDLELLQVNMNNFEIMWKIQVKQFNFDGKGMKKINWEKSNILMPGSLICLTNIECYPLIFGVITKRDKYQSEYEYGLIQLEFSFIDPNTSIMQFFNLFSQKTILIQFDGLVEPIHYYLQALKSKTSLPFQNIIVENQNDVKYPEYLNNKQKFEIDLSKNDQYRGYIFNILNPIWPELNSTLDGSQLNAIKIMLQKQVALIQGPPGTGKTYCGALAVRILYQNLLNNNFPILIVCYTNHALDQFLEHIIKFVPIEDVARLGGASKHPELQKCQIRCRQKIDFEFQIFNKLKQKISKLIERMLKYDYIIRPKDIKKYWPELQNIIIKKFIEDNDLNYEFFINQQEQDQLVNYWIECDNSEDDTLENSQQDEMSNYFKMDQFYQESEQFKHLIKSDCLQQQQFYSEDEDLNIYLDSDDEAHSYNNYYKEANFDLIAVDNAIKQFNEKQQNFQLLHYDVQSIQKIINYVFHQKGNPWYLNPNDIEQILIYLESLRYQNDSVKFSRLYQQFIDLSNNLKELYDENEIKILNSMKIVGVTVTGVAKQAKKLEKLNTKIMIIEEAAEVLESHVASILTKNLQHLILIGDHQQLKPKLKNYSLQMKLKNADVSLFERLFFNDIPSAILTQQRRMKSKFADFIRLIYSNSYQDHPDINKQNNIEIQGLEKDLVFFNHKWPEASNEKSKENKLEAQMIFEMVQYLCKCGYQQTQISVLTLYLRQAQVLKSQFFKSGYNNIIVQTVDNYQGEENDIVILSLDIFKQKIELMQLYQEQEQVYTYLEILILFIKLQSMVVYGKKQYNWLXFDLIAVDNAIKQFNEKQQNFQLLHYDVQSIQKIINYVFHQKGNPWYLNPNDIEQILIYLESLRYQNDSVKFSRLYQQFIDLSNNLKELYDENEIKILNSMKIVGVTVTGVAKQAKKLEKLNTKIMIIEEAAEVLESHVASILTKNLQHLILIGDHQQLKPKLKNYSLQMKLKNADVSLFERLFFNDIPSAILTQQRRMKSKFADFIRLIYSNSYQDHPDINKQNNIEIQGLEKDLVFFNHKWPEASNEKSKENKLEAQMIFEMVQYLCKCGYQQTQISVLTLYLRQAQVLKSQFFKSGYNNIIVQTVDNYQGEENDIVILSLVRNNQNNKLGYIQIENRINVALSRARIGLYVFGNFDFIYKASINGSLWQKIIQLAKIKDYLNDYINTKCIPHGNLQKIKYPEDWKKIKPFFCNKKCLKMYSNCNHLCNLDCHISDYHSEESCPFQCDRVMQCGHKCKLLCKDKCQCKEIIDLQLSCEHQIKTICGTNPQTQKCLKEVQIQYPKCLHIKKFICYQRDNYIFCQQQCERILNCGHTCNKICYVDCGNCMQECNKVRPCGHKTKCKNFCYEDCSPCDFPVQTELDCGHTIEFPCISQKKDQQNHICQQNCRKKRECGHYLQICENKCHEQCSPCYFSIEKQLKCGHYVIVNCFEADNKEQEIRCIEACQKPRQCGHDQACLNKCYQECSPCQVMIPKILNCGHSIQIECYKQNQEYQCQQTCTRKRDCNHDQQCTNLCYEQCSPCIQIINHTLPCNHNHYFECHSFNISLQQFICHEPCLLQRSCGHTSVCLSKCYENKCSPCLQIVNKKLDCGHIIEIKCCEFQQTQICQEPCNKIRECGHINLCKKLCIEPCQPCEQAFLKTLPCSHQCLVNCYQQSQPINCKENCIKIRECGHYYPCTNLCFETCTPCKSVVLQKLKCEHYIDIECSQISLVQLNYKCSQQCIKTRMCGHNYPCYNICYTNNECTPCQANIQKNLVCGHQFQIKCFQRMQKFNILCDMPCKKQRSCGHNISCQNKCYEPCSPCTQLCEVVLKCGHYQSIKCFQKDDQSVLNKIQCLFPCTKKSNCGHNSNCTKQCYQQCDPCSQQVQILLNCGHFYKAFCQQDPNTIKCQELCKIQLNCGHPCQQICGTPCLRCEQKILKIFPKCFHQLEIKCYENNQILLCQQQISVSKSCNNHFAQIKCCEQDQSILECQECKNKNCKIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TP18</accession>
    <name>A0A8S1TP18_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0230311</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TP18"/>
    <dbReference type="OrthoDB" id="10460621at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="288"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="95"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="464"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="543"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="712"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="802"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="225"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="302"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8152926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="911" mass="109322" checksum="27B6422AA02E3B3E" modified="2022-10-12" version="1">MEEWTEDQIYKIILKISEEQSDSFLNTMEYHQKFDGNNRISYNQSRQLNLDDWNLRKGRCNKRNFTFDQLNSMKVENEDQISQYEHKIMVMNQEYEQCLSHIKELENVNENQNDQNEKLFDQIQRIEDELSKGRLKYTQIVNELEKKDTQLKEQITSIQNNEQINQQIRKKNQDLEELINRLKNEIHQKDTQIGKLISKIKDQDDKLQLKDLEIKNYMKESEKQQYASIEQQQTQHDSIKKEKKRTINYMTRNKSNTVLLKSETLHSRSQSHYIGQFDLEQFKEQCAASVKSQNSESEDDQTDKKTLKQENDEQQELEKLIVELKSNLEAKTNQLQIVENEKKQCEAQIITLMSEISVFKIKEKKMTLQQKQQEKRLTELQSEKNKFQLEQEQLEQRMNEIQGQHQQKMKLMSSVINENDQLKKRLSLNLGRVETQQQTDKDIIKIKQEYDDMIMEMQKQNMDLHFVNEELIEENNKINEQIKQISEQKLNDIVKIQQLEKQLEQMEVELITQKQINLKDDKNIFEDKIRIIKQELLIDYDIIDQLEKNKQKQSQNDRIMENQQNYLKMELQEQIKQLELANNQLKQQIQQYQILKQEEEIIIQNQVEIQTIIQELEQKNHGLQEELKEKEALKLKEIEIINLEIEKGVHHLLEKNQQIESQQAMIKELQLKTIQIQQEFEQVNFQSLNQIEQLQIDVYQKENVLQQQHQSISSFEQLILQQNTQLEQLKNNTFIQNQEQQIQQLYTEIHALKLQIEQQDDHQKVYESILNKQIEKLKQINLSQQTQILSLTQDKEKLQSICSQLKQIENILQDELHKMQDKIDELCIELDKVQQPSTRSSSISSQNKTLIYEQFFHLTSQSVQMNSNHQNAFDVKLKSYYSEVVNQGVPFHKWSKWLEDKLKEELNKFQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TPM3</accession>
    <name>A0A8S1TPM3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0250101</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8153928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TPM3"/>
    <dbReference type="OrthoDB" id="307716at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="370"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="430"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="381"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="431"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="448"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8153928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="501" mass="59642" checksum="52BB4FE558D1A657" modified="2022-10-12" version="1">MSAFRAFMIYDYQRSFFFLFIRRGFQVSPNWNDCRKELENKLQEIVKEPQDTFQQSSEYGMFRCKYDKKNKCYFILLSSNEVQEAYQNQVLDEIYQNIEKEPNYSKIKKEELEQKENEIIKNLLETKENEYIQKYGKVKAFQEPHESKINGQIQQQSNLQSQQQMGSSIIGTQHQRITSQIPNTSAIYNKSQGFNSDKDLSSDTLIKLFRCFMMWDYNRQFFFMFIRRGFPIDKTWSQERVKLEEQLRAKQLDPNDPKAKPKEPPELFDYRGNFGQYKAKYDKKTKCYFILLSRFKIKEDPQIKLLDALYDEIQKVDKYITLKYDVLEDKKKKQLEKIIDEYEKETSNQVNQRFIDTKVLEQKLGFLTSPERNIQRDTEESAIQQNGRVSNNETSQIQMQMLTSNQIQQTQQQNHIINSSKLLQSKPIYNQQQPQPQQQQEKQEIQNDSDPIVNQDNPDLISNQDNPENIPITSEVGQQAQRLSLYYTKPLNISSYIKSRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TRZ2</accession>
    <name>A0A8S1TRZ2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tetratricopeptide repeat protein 21A/21B N-terminal ARM repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0250299</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8154292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TRZ2"/>
    <dbReference type="OrthoDB" id="343875at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056833">
        <property type="entry name" value="ARM_TT21_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25062">
        <property type="entry name" value="ARM_TT21_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13176">
        <property type="entry name" value="TPR_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="108"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="176"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="246"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="280"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Tetratricopeptide repeat protein 21A/21B N-terminal ARM repeat" evidence="5">
        <location>
            <begin position="329"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="348"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="419"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="453"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="521"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="555"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8154292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="829" mass="96400" checksum="CDE6A1F509DFDAA0" modified="2022-10-12" version="1">MGSGATKEKKDEINEIEHHQSSQTSIGKAEGSPQIFTYKNNSQLSKAFGLLKKQQYKQAIESLDKLIEQNPNLADAQYLKGLAYLGLNDLQQALIYCKAATDIDTQHYHALAEIANIYTLERKYEEALTIFHKLVELNEKSFEGNFGIGFINLMLNNFEIADPYFKKALTIKGKDKAALLNYGHLLIGQQRFDEGLQYYEEALKVDPKYADAINAITNIYLRQKKYEQLFEFLDGAGDQSIPKVKSVVLNCKSQAYYGLKQFDKSIKYCQEVLEYDPNNIDSLYGMGMCLYHNNQLHKAMTYFNQIIHQNPYDIKTLKMIIKISSTLQLYYQLSDCCDKIIQLGLGDQSIHYYRGLALMNQKEYQMAIEDFNKTLIYDSTSIIALKNKAICLTQLKEYDQAVQCYDMILKQLKDTSEKSDLFYEKGYCHLLGQQFFSAKNNFDSALQLKPKNEDLMLKIANAYRDNSNFQPATNMYDRLIKMKPKNPIFYAEKAELQNKQGNYKDAKLLYDQAISLQGDNAQFYIQRAKIRTQVQEFDEAISDYQQAIKINDQDSELLFELGQLLYMKQNFEQSVIYIQKAINLNEDVEKYHLKFAQVLQMQDLDQEAIEHLKNIIVKHSDFDNCKNLLDNLNSSPTSFREYTNVFTYIVISMFSEGMVDENQQIDRVRQESLRILQENLKNFFPNIPNIFEIIRCLMTKKFEYNLINNPKEMRRILTLLYKNKFEQQNQCILDIIETAKRIAKLRENYLKNDDQNIDNSLQETFLQQCSEYQIGEFQCKASALAIQDSVALITGIILSYNRLIQTQQPLKNQIYDLMKNGSLNKLKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TY10</accession>
    <name>A0A8S1TY10_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">W2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0300313</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000030">
        <property type="protein sequence ID" value="CAD8157725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TY10"/>
    <dbReference type="OrthoDB" id="424572at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04197">
        <property type="entry name" value="eIF-2B_epsilon_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035543">
        <property type="entry name" value="eIF-2B_epsilon_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051956">
        <property type="entry name" value="eIF2B_epsilon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056764">
        <property type="entry name" value="LbH_EIF2B3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003307">
        <property type="entry name" value="W2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45887">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45887:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25084">
        <property type="entry name" value="LbH_EIF2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51363">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="W2" evidence="3">
        <location>
            <begin position="399"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="396"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="404"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8157725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="592" mass="69637" checksum="CE6433358BC3F6FD" modified="2022-10-12" version="1">MSEFYAVILADSYDNQFNHLTNNIPKSLFPFVDDLIIEHQINWLSKNEIDQIIVLYRNEKIAEYFNNRRRPGRKTQKIQLINILDSKSSGDALRELYSHGIIQQDFLLLFGDVITNISLKEVINKYHDQRKEDKMNILLMVVHQGIQQYDEERYLYVLESDDKLFQLTDLQQKQIIINKKHITLTKGMPCKYVIRSNLIESGIYICNRDVLKSFQEYFMFAEIKEDFIKYMTTTSDIQEEKIHLYISPKIQVSQRIFHAQSYHNACLKYLSRFFFPYCPQQQYYVDFFKYFAGRVDQKSKISDQCFIGQNTDVKQKVTITKSIIGKNCKLGIGCEIINSILWDNIEVDDNIIIKDSIIASGCKIKNSIQSQIVTIENQKNVKQLLSFQKYDDNAFQSENSDQESDSHELHQKKPKENDDQGLKEDMIDIVANCKTVSQVKEAITNVDTIKVNSNIAFENIVIAFMAAFLDTLQPDVDSLKNAYALWEEFIIRFVSETEVVCYIKSIETFCRQNQQYHLNQFLQLSYKHQILSEQVIINYQNQMATIEDDQFAKDFVQSSQQFKEYLENIILEQEQQQNQEGDGEYEYEYVYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U115</accession>
    <name>A0A8S1U115_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T0320232</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DEAD box helicase family. DEAH subfamily. PRP16 sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8158935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U115"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000001">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000007">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000615">
        <property type="entry name" value="pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF91">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR ATP-DEPENDENT RNA HELICASE PRP16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="327"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="512"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="946"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="46"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="64"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8158935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1059" mass="121904" checksum="4D4C29542F86F0AE" modified="2022-10-12" version="1">MERESRSRDDSKGRQAPFYRESLYRSSSSVSSQFKVPSGPMLFKGTENSSVQKNAQPQFEEDADGFKKPPARSEKLEEERKRNKLLNEYTAFEWENIEQESDRKWYDYDEDDIGNASQQDWGGEVFKGQKEEFKFEEYSKRKGQTVRQSEKNQEQNRWELNRMIASDVFKRKADAWDFYEEENEKRVVIHVHDIKPPFLDGKVVYTTQLTQVQIVKDPNSDMAKLAKQGSEVLMLMREKQDKTKMRERFWELSGSKMGKVMNLDRKKEQDPDRHLLNEDGDYDFKASSRYQTALQRVTQGQSDFARNKTIKEQREYLPVFHCRSELVQLLHDNRVSIIVGETGSGKTTQLTQYLYEEGYTNTGVIGCTQPRRVAAVSVAKRVAEEMGVELGSKVGYAIRFEDYTSKDTVIKYMTDGVLLRESLQDPDLEKYSAVIMDEAHERSLNTDVLFGILKKVAQRRRDIRIIITSATMNAKKFSDFFGGVPIYKIPGRTFPVDVRFEKAPAQDYVRSAIKKTIEVHIQQPPGDVLIFMTGQEDIETTCYLLAEELNKLSEATPPLLILPIYSQLRSEEQARIFEKSEFRKCIVATNIAETSLTLDGVKYVIDTGYCKMKVYNPRIGMDALQVTPISQANADQRKGRAGRTGPGICFRLYSSLNYRQDMLENNIPEIQRTNLANVVLLLKSLNINNLLDFDFMDPPPQDTILNAMYQLWVLGALDNVGELTELGRKMSEFPLDPPLSKMLIKGDQLGCTEEILTVVSMLSVPGIFYRPKDREAESDAAREKLFVGESDHLTMLNVFEQWKRHEFSPEWCNDHFVQAKSMRKVREVRAQLKDIAGKLGLKMSTCNFSYDVVRKAICSAYFQNAAKIKGVGDYINLRTGMPCKLHPSSALYSLGYAPDYVVYHELVMTSKEYMHCVSAVDPQWLAEMGPMFFSIKEDGETRASRIESEKKSKREIELSIEKAKREHEMKSEECLRRREKEDRERRLISERTATLGFRPSRIQTPLRQQISSIAATPLRSECESVISGMTSIQAKQMRMAYYDDEEEQNVGKNKIPKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U270</accession>
    <name>A0A8S1U270_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0320061</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8158628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U270"/>
    <dbReference type="OrthoDB" id="10266426at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006862">
        <property type="term" value="P:nucleotide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044712">
        <property type="entry name" value="SLC25A32-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45683">
        <property type="entry name" value="MITOCHONDRIAL NICOTINAMIDE ADENINE DINUCLEOTIDE TRANSPORTER 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="5">
        <location>
            <begin position="270"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8158628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="360" mass="41541" checksum="7E13A237C60ACE33" modified="2022-10-12" version="1">MSGKDFSQFLSNKDNQSIQESVKKSIDPNLFINEQTLKQIEAETNKHFWINYFTQSILTTFIAPLTTIYTSLQLSVIPHQNKYGDLPDESGTKTSTQLVKTTRDIQPHEKRKFELLIKSGAVGSNKPFRAPIYTTYREAIQGLTNQGWQAFYKGNLIGIIHLLANSHLKIKLLSDLDFKLGAMWHEGNQLLRQAVILGLATVSDVISNPFMLFQSRFILQNRLPNFYLYKNLLSAFKKHVRHKKEYFQGSTTYFYKNGLIFLIQVPIQFSQKLEQFQMLYLYLSYNLISYPFLTIVRRLHCQSTLPGMIPIRYSGPFHALRLITQEEGIKGLYRGFGLYLIGSGLLQYIMFGTQIDQGDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U2Z7</accession>
    <name>A0A8S1U2Z7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0290301</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8157026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U2Z7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8157026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="229" mass="28449" checksum="E0829522C165936C" modified="2022-10-12" version="1">MLDFQFLCNFYNVDTYLENQSIQYINHSNFSNITKAEILEPTSICILRELINFKYLFPYPESQICSRIIQNQFQNYRRSKSSQMFNRYYQTKTRFQETQQLFLNLFMLMNQDSIYYKLIMFLFIISSKIIYQKWQDHLQLKKWNISFSKKLNNLQMNKKIDYNTNQKQIKQQQTEKSIENQLKKLLLTYKNCKRTQSFINKQEDWKKDQKYKKRIHKIFRIRIYQQLHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UCG3</accession>
    <name>A0A8S1UCG3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RCC1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0380110</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UCG3"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051553">
        <property type="entry name" value="Ran_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982:SF1">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RCC1-like" evidence="3">
        <location>
            <begin position="2"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="132"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="243"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8162550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="404" mass="46061" checksum="9AD4318821CF4FCA" modified="2022-10-12" version="1">MKVFAKGLNFMGQLGQGNFKSLEHFALIPDLQNMGIKQLEANMTQSFALLQDGQLLHWGWQLDTMTENRTLSFYKRNPTLCKLWQKYSPLGTMLSMRGGIVQPVLLNKPIIDAPIKSFQVGGSFIIIQMNDGVCYGMGENYKGQLGNGELKYQFQFKQLQLPTKDKVIQVGCGYQHSLLLTEGGVVYGSGKRNHYQFTETRKNDQDQIYVEFTQHRQLMDIRNQKIQRISSGAHHTLLQVSDNRIYACGLNIYGQCGMNNEIDRIKEPTEVFLDLQPDESIQQIVSGQAHNLVLTSNNRLIFFGCLLHNQMGIKSKFDFEFSGQTEINLPLEPDEKIVKIYAKFDRSAVITSKGDCIIWGGQDLRYMDIPYYETYTRLIPELGLENVKIQDIALGFSHIMVLTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UCS2</accession>
    <name>A0A8S1UCS2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0370098</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8161927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UCS2"/>
    <dbReference type="OrthoDB" id="284608at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8161927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="182" mass="21331" checksum="C65AF0933007A285" modified="2022-10-12" version="1">MNLDQAFTFGQDCYNQDQINPSKHPKISKKSPFYSIVKPTTSQKNYRIKDTSVQQLAQTIQKNTNKENYNSHQCNVVLPKHTRNPTDLNLIRFDTSPNSQLEISNNSRGNNSKQENQRRLSFNTPNQTIRTSLPPVNNNEKYKKIAIQMKELIQQQKYNYEIGDYEQLEFIFQKLNLLCDQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UDK4</accession>
    <name>A0A8S1UDK4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ammonium transporter AmtB-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0380029</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UDK4"/>
    <dbReference type="OrthoDB" id="534912at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097272">
        <property type="term" value="P:ammonium homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3430.10:FF:000008">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730:SF6">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="206"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="242"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="296"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="329"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="366"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="9">
        <location>
            <begin position="23"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8162424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="420" mass="46080" checksum="8FC9A3CBC7A0B1FB" modified="2022-10-12" version="1">MTDLGIAIERMEQLKESIDIGYLCICAAFVLLMQVGFAFLEGGCLRYKDMQSIMLKIFMNTCVTIVIWWIVGYGIAFGSDSKEFVGSSVYIGFDMKNSKHLAEFAFQTAVASAATSIVSGGAAGRMTYIGYFILSVVFSSVIYPFCAHWGFGNGWLMKLGYHDYAGSGTIHASAGMGALMTTMLLKPRNQRFNPKYAEHFEPNNPIYITLACLSLWVCWCFFNGGSSLGIADGASVFMSRAIMNTFIAGASGALTVFFIFYLMNIDQTNRYSLVMICNGNLAGLVAITGPCDNVDAWAAFVIGILGGMMMVFANRVLFNLQIDDPVDAISIHFACGMLGANLVSWFDMDTGIVYGKGGYQFAVQMLGSFIYIGWSALIHLITLLFIDKIGFLRVTLEEEYEGLDLATMGCCAYEMVEQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UDW4</accession>
    <name>A0A8S1UDW4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0390041</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UDW4"/>
    <dbReference type="OrthoDB" id="303888at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8163098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="177" mass="21437" checksum="330E4C50D3FDBA68" modified="2022-10-12" version="1">MKFSVPLSHSPSNFSKQLIQQQRQLCLFKVRKQLQNLNDPFDSTEISRALRNLETQQQRKQLLDSIIKMRETKRTQEEQQKQEQLQRLWVKANQTNSKKLNTNYVYHRDLEDFESIKQSRYRFESERIENFYLRNEGKHHNFHRVSSLACLTTDLEKKKKLIPLITRKDRKNFTIVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UK92</accession>
    <name>A0A8S1UK92_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPENT_87.1.T0410106</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UK92"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000315">
        <property type="entry name" value="Calcium-dependent protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001779">
        <property type="entry name" value="Probable myosin light chain kinase DDB_G0292624"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="11"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8164583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="314" mass="35697" checksum="65E7A935C82DCF95" modified="2022-10-12" version="1">MQGHEDITKVYTFEKTLGEGAFGVVKRAKKKSNGDMYAVKIINKDNLSNEDLQALQTEVEILTQIDHPNVVKLYEIYEDDTNFYMVMELMTGGELFERIVEKDHFSEKEAAATLRPIIDALSYCHKMGIAHRDLKPENLLFSSREPGALLKVSDFGLARFVTNDEVMMTQCGTPGYVAPEILSGHGYSEAIDFWSVGVILYIMLCGFPPFYDEDNDKLFKIIKTGQFSFPSPYWDSISNEAKDLIKGLLTVDPSKRFGTEKIMKHPWLVNNTAKSIPNIQNKMKEFYGSATMKKMGNFVKLAQRWGKLSQIKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UKJ1</accession>
    <name>A0A8S1UKJ1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0420174</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UKJ1"/>
    <dbReference type="OrthoDB" id="277011at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000121">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="2">
        <location>
            <begin position="479"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="383"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="383"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8165360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="679" mass="78779" checksum="D1B9C2FA21637998" modified="2022-10-12" version="1">MDHKQKNQMKSQCYLHGYPSDCQYQLQDLYNLIRDDEDSITLERIFKMLRTWQYEVPFSNLCLLLRKANQACHQNAKNYLNEVAFHQLLHNPDIQKLFKPKNDEIYSTPPTIEGLFKRMGEQIERKRLHEKLKREGISNKEKFNFMLQLLQVGSPRKEKECSITMEKTKSVRKVIDDDFNFIKQQKKSKRKAESMQDPQNPPKFTKLPNTIFHAPTSPNGGCTIGSQCQEVEQYYSQIIGQPFNKVTLNLKYMKQQRYQMDFNLSKSNLLQKIQPNREQSAKKLSNSEVNAAIQKYFQMLKKKEGKSPSPIISQTQSSKILSQLVTKVSPSLDINVPIKPPTPSGTQSYRPLSEKKPHHRPQQSVGQSKDLILQKLQTALFQKPKTQSQQVTPKSGGKGSASVNKYFESTKKQQGEFHEYYLSKVKVAFSKPLKDDYFSRMYREHFFQTYQGICVASYLQPVDPNDLQNKKVKLKQKECYKNKITIVFDLDETLVHCNESLVIPSDLILSIQVSPQETIKAGINIRPGAVKLLELLVNDFELVIFTASHPCYAQKVIEHLDPQKTLFSHSLYRDNCIMTTGGMYTKDLRIFDRPLSQLVLVDNASYSYAWQLDNGIPIVPFYDNKEDRELESLLKYLKGMQGCKDVREYNRNNLRLQYFQDPSGAGMVFEKLFQQKMEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ULK3</accession>
    <name>A0A8S1ULK3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0420139</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ULK3"/>
    <dbReference type="OrthoDB" id="7318948at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="60"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="105"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8165294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="332" mass="38829" checksum="42CB83747BBCBBDF" modified="2022-10-12" version="1">MQELSQNTDFSYKLVSNYHQAQSSQAIAINRYNTLLLVSALKKIKVFYFKNKQLKYNQIINKHSDEVTTLNFFQIKPWFISGSRDGSIIIWPTSLMKYPKYIMKFKGHSNWINCVVLQPLNQDLIISFSKDSTIKFWSVSNTSCMQTIRLINQDIYSSSINNQGNKLIACSNNNQILVLEEISCFKWIITQRIVVQNFGYRLSFITNNIFAFQSHKQANLQIYYLNALNQTFSKMMDVQIQGGDQPCNLYFPMIYVPSKNCLLVKNGYKLNLISIVLSKEEELLECKLQQVIDFKKVLWSQIFGTISQDGQFLITWDYKSFLIQIREYVHHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UMW6</accession>
    <name>A0A8S1UMW6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0430109</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8165754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UMW6"/>
    <dbReference type="OrthoDB" id="294692at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF82">
        <property type="entry name" value="KP78A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="20"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="404"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="415"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8165754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="427" mass="49783" checksum="001E7C79619C23F5" modified="2022-10-12" version="1">MSLDIQSDYLDYASVLDDKYYIIDTLGVGRYAKVKLAIDIQDKQKYAIKIMKIDPTTNQNKLSQFINEVSHQAEFEHNNIIKIIQCSINGKYRKVDGRLCQVSYFVMELADQGELFELLEQTHQFSEKFSRGIFSQLIKGIEYLHERGIVHRDIKSENILFSNGILKLADFGFSAKSVDQTGAKVQFEVQQYLGSPEYNPPELSNTIGKQKYYIPESADVFASGVILFSMAMRSAPFKTSKNTDPYYSLLKQDKQKFWQVFQELNNNISHQFKDLIEKILEENPQKRISLEQIKKHPWMQGQISNLTDFKTEINNRYEIVQNKMKAKITFRRQQKFTNRRYQKRIEMSIPNLIPPKYLQESLVVIDDINDKLYKRLLLQAYQDQMSQKAQPTTDFKIIEIQQKTKSKRELEGSKKSSPATPPNDSDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UN60</accession>
    <name>A0A8S1UN60_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0430160</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8165854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UN60"/>
    <dbReference type="OrthoDB" id="418595at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003938">
        <property type="term" value="F:IMP dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04601">
        <property type="entry name" value="CBS_pair_IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00381">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000424">
        <property type="entry name" value="Inosine-5'-monophosphate dehydrogenase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005990">
        <property type="entry name" value="IMP_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001093">
        <property type="entry name" value="IMP_DH_GMPRt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911:SF122">
        <property type="entry name" value="GMP REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911">
        <property type="entry name" value="INOSINE-5-MONOPHOSPHATE DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00478">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000130">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01240">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0129">CBS domain</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="179"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8165854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="501" mass="54713" checksum="9685BEAC21ED23EF" modified="2022-10-12" version="1">MLSSNWRNGIQTALTFDDVLMVPQYSTIESRTNCLVETHCSQNIKLKIPFISSPMDTVTETEMAIHMAGNGGLGVIHRFMTANEQVEQIKKVKRSEAYIKNRPFVVGLNFTLNKVLSLADEWKCKTFLVTDDNLIEHNEDYEETDETGSPRYKSLPLLGIITNRDCYEQPLTKLVKELMTPREKLVTLHYLDVSKENARQKMLSHKLEKLPVVDDKNNIKGLINLKDLKIDSELKVLDQMGRLIVGGAVGATDEEITRAKRLVNSGCDVIVLDVANGHSQLAIRTVEQLKKEVSVDIIAGSIATGDGARRLIQAGADGIRCGIGNGSICITRVVSGCGVPQFSALMDVAPVCKEYNIPLMSDGGNKNSGNMCKALAVGADCIMLGRLLAGCQESPSKEIYREGKILKVYRGMAGFGANVSKAQRTGKDEPSSTTFAPEGVEGYIPFAGKVSIVLEQFKKGIQSGMSYCGASNIAELQKNVQFIQMTNAGFVESGVHGITKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UNN1</accession>
    <name>A0A8S1UNN1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphodiesterase</fullName>
            <ecNumber evidence="1">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0420165</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="1">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UNN1"/>
    <dbReference type="OrthoDB" id="302125at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="2">
        <location>
            <begin position="200"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8165342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="530" mass="62481" checksum="0534E418A2C44C83" modified="2022-10-12" version="1">MADFVKELDLMKQYYNEQIMKLQDYQECEAQQQLSSRANDLLKKLRLIKYQQLLIKNNTFQKGIRKPKKMNSEIHQNAFSLKQLPEDYIQDEQSSIQQDISNNNFQNIQNDNHPPQFTRKSQRLMTSATDQNFRINENTNSPSFSEKNIEEFKITTEDIDDLLNLLSGKKESIWLPKFLRSNQLINEQEGFSVDAKQFLLSHFTQKQTSFEYIQDYQDKVQEEELEIIDFNANFLQYCRNKLQDNMQTKIFFENTINFFKRFKIAQTFKINKSELIGEFALKVDMSYHNNLYHNSMHALDVTNSTAFFLENGLNELLDEFEQACLIISSMVHDIGHPGLNNGFMMTNRCKLALLCIYINMLDNDQSVLENYHSFLLFQIISQDQFNIIQNLGQVEQKGFRKYCLNLILDTDLTRHFQLMNRFQNYLELTETQDIDKHLLMSICIKCADVGHGAKQLKIHKLWSRRIIEEFFLQGDLESYLKVPVSPMCDRKQCIYKSQEGFLKAIVLPMFNAFVTLLQKYMIVQLCIVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UQC5</accession>
    <name>A0A8S1UQC5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0460112</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8166875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UQC5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8166875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="77" mass="9728" checksum="F408C9DAA20B81E3" modified="2022-10-12" version="1">MPQNYENYHKQLSFYTDSFYEVEIYQNVNNNLEQRLTYYHLQKVQKQQFIKLMKFENRTNPIYFKLQSSFEQYHNKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UR36</accession>
    <name>A0A8S1UR36_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0440055</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UR36"/>
    <dbReference type="OrthoDB" id="1926212at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050498">
        <property type="entry name" value="Ycf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE SPINDLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13431">
        <property type="entry name" value="TPR_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="50"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="152"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="250"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="284"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="318"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="352"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="386"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="420"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="454"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="488"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="522"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="556"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="590"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="624"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="658"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="692"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="726"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="760"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="794"/>
            <end position="827"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8166262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="876" mass="101858" checksum="2066A52EA2861E92" modified="2022-10-12" version="1">MGNKNQKEKDNDSARAFEHYSRSKKLQIAYRTEKAILEINKALNLNPIFAEAYTLRSELYEQMKLYDKALKDVNCSLSINNNNAESYYQRATIYWRQKKLNLALKDCWKCIQINANFAMGYSRLGTIMGELKEIEKELEYHTEAIQKDCNCYYAFMCRGGVYMDEKKFDQALRDLNQAIQINPQYSLAFYNRCFKLIIQKACSIQILVSKTKHQMTVIKQSNWIQNLLIPIMIEALYDFNKAIQLEPNNVIAYYKRGNFYQKIGEYDNAINDYNKAIQLDPNNAIAYLKRGNFYQKIGEYDNALNDYNIAIQLEPNDASIYHNRGLLYYNIGEKDNALNDYNIAIQLEPNDASIYHNRGLFYQNISEKDKALNDYNKAIQLDPNNALTYHNRGIFYQNIGEKDKALNDYNKSIQLDPNNALTYLNRGIFYQNIGEKDKALNDYNKSIQLDPNNALTYLNRGIFYQNIGEKDKALNDYNKSIQLDPNNALTYLNRGIFYQNIGEKDKALNDYNKSIQLDPNNALTYLNRGIFYQNIGEKDKALNDYNKSIQLDPNNALTYLNRGIFYQNIGEKDKALNDYNKSIQLDPNNALTYLNRGIFYQNISEKDKALNDYNKAIQLDPNNALTYHNRGIFYQNIGEKDKALNDYNKSIQLDPNNALTYLNRGIFYQNIGEKDKALNDYNKAIQLKPNNALNYLNRGIFYQNIGEKDKALNDYNKSIQLDPNNALTYHNRAILYENIGEIDKALIDQNKNIQLDPNNAIAHYNRALLYQHKGWQDKALNDFNQAFQLDPNFVNAYFGKGLILQDLKQFEQAIICFDKAISLDPNQINIYYNKAISLEKLNQIFQAIKYYKLALTLNHPSQNQISQKITKLQGCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UR77</accession>
    <name>A0A8S1UR77_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0440079</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell outer membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="294016at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003368">
        <property type="entry name" value="POMP_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02415">
        <property type="entry name" value="Chlam_PMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2346"/>
            <end position="2367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2408"/>
            <end position="2429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2456"/>
            <end position="2476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2488"/>
            <end position="2507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2544"/>
            <end position="2571"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2607"/>
            <end position="2634"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2683"/>
            <end position="2701"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2997"/>
            <end position="3018"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="3059"/>
            <end position="3080"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="3107"/>
            <end position="3127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="4038"/>
            <end position="4059"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="4100"/>
            <end position="4121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="4148"/>
            <end position="4168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="4180"/>
            <end position="4199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="4236"/>
            <end position="4263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="4375"/>
            <end position="4393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8166302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="4488" mass="526157" checksum="BD35A0D4A4C8D9A0" modified="2022-10-12" version="1">[encoded data, 4488 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1USI2</accession>
    <name>A0A8S1USI2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mitochondrial import inner membrane translocase subunit TIM50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0460242</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the TIM23 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TIM50 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8167127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1USI2"/>
    <dbReference type="OrthoDB" id="445750at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000551">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="1" id="KW-0811">Translocation</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FCP1 homology" evidence="2">
        <location>
            <begin position="132"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8167127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="337" mass="40115" checksum="7BA52BBC04B156EB" modified="2022-10-12" version="1">MLSKYSIRSLTLLRFPRYRFSQQQQQQKEQQDEWDERTIEAEEANPTLENKEKAFSYFRLFSRIFWWTTSALFGYNLYLNNYKTDPTQELGYQKQINDAAKYCQDQYQAFYDFMTKPAIDKLLPDIPELPFGYEIPKTLVLNISGTLLHMDYVFGVGGEIKRRNGLQRFLEKLPKMYEVVILSDDETMFTQQITQKLDPTRQIFAGAFGRESMVFEKGRYIRDLKYINRPLNRVIVLDSDPERMYQYQDNGIFIKPFDGKQNDEVLKDVLLLLEHLSKPQIKDVRAELRKFGNFDPQVKYLDEVKAREINIKQTMNKGLFGIMNQRKNPQFEQSRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UTX6</accession>
    <name>A0A8S1UTX6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0490182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UTX6"/>
    <dbReference type="OrthoDB" id="298843at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="122"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8168570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="355" mass="41898" checksum="400820EAE30A41E7" modified="2022-10-12" version="1">MEKKIIEFIQECQTNIGNCQDLSQMEEIVHQLISIVYFLIEQGQQTFEEEKLKYQALNQEFFNLQTLYNQALQEIETLKVHHKQDVEEFSLIEDRMIKQEYFIEKEKDEQINYMKSSTYEMMKSLKNQQLQLENVKKQNEDQQMEIKELRKLLQQEKQSNIGSALDHEESKLQTYLNIKGPQYSSSSCDSVKLKQQESCSSIELNPQSNQKENIQTENCSQTILRTNSIREAGLHLKYTHCHSKNQSINWVKLATIAQTQQSLSSNSDDEEAVCQKSQRRLSKDLSTECEPILFGSRNPYKDFYTLISQSVKLNLNQTKYYVVNVDMLYDQFLAEGIPFHKWYKKIKDYIQKQVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UVX6</accession>
    <name>A0A8S1UVX6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PPENT_87.1.T0490194</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UVX6"/>
    <dbReference type="OrthoDB" id="9977870at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031090">
        <property type="term" value="C:organelle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000051">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="184"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="221"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="3"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAD8168594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="251" mass="29518" checksum="A83FCCA16B1FFADE" modified="2022-10-12" version="1">MNCQICFIDKQMIKPGDCSHQFCKECIIMYLKETINNGSVFKITCPACTIEYSALIIKQYCEDLYPKYLELKQKAIITCSVCKEDIKTHKCGSKVCSKCGEIHTGYCSQRCPNCLIPIEKISGCNHMICKCKYEFCWICKGKYSKYHYRLWNLFGCPFPGSMMRTIEPLQNPMLFRLLMILPKILAFMLLFCILLLIYPFFCFIITVKFHYQQFKIKKSRILLVFLFPLTYLMHFFYKGIFAVQHKLHSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V5X8</accession>
    <name>A0A8S1V5X8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0560137</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000056">
        <property type="protein sequence ID" value="CAD8172184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V5X8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8172184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="125" mass="14724" checksum="D9CD58BDDB91AB02" modified="2022-10-12" version="1">MLTPQFSYDSNTNFFMDDHYAQQLQSTIIMNYQLQLKVGELRLLKILINSLIWLLLIRLRQNQILCLVHSDSVYVIQLFTLVKILQFVVQISSNLGLQLLKFKSTNNGFNFKLQALKIQFILDYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V7L9</accession>
    <name>A0A8S1V7L9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0550239</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V7L9"/>
    <dbReference type="OrthoDB" id="295862at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8171839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="528" mass="62868" checksum="0B1F941D204E8856" modified="2022-10-12" version="1">MLSKFQLRPSTLKSSGFTIYEATSNSEPYFAYFYTAIYDQAYRQQDLEPKSEFLREPLKITNPGSKYVFFKYYGNFRLADITKKLQEVEVINILSQLIQAIYALHRCKLMGRCFNIYNILVTNISKGNFCIKLMDFGFGCPLEYQPLQSELNSPNYDYQFDLFLIGRILFFLLTTQDIPEHYIYESQWAETQKKIDNSSYSKKLQGICKNLISPTIQNRYCYENFISDFLLLKEKQQQFMQIKKFYSESQETFSSINMEIKKRAENSQVADNIAPPYLSDQSLDNIIFFFKPEKNQIHKYLNFKLFNFTIINQAIENIKLKFISSDHISVPLSIFVLEKIKCIFFQKIFAMFEKKQFANFNNEEINNFVTSSQYQDCYILLKNLNYLQQQLMPNYLDNLLKSLQSNYPDYQNHLLYQDIKIFLYLDDFKLSFDEIKNFYRLPLNNLLKKIENLDDNKSNRQFRLVIFMCILINKIGESKNLNKHYKTIMKSLQLQEIDSPLQVNDFLVKANIEELNEQFEELRILYFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VFC1</accession>
    <name>A0A8S1VFC1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0620165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8175085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VFC1"/>
    <dbReference type="OrthoDB" id="282210at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035866300" evidence="1">
        <location>
            <begin position="17"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8175085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="107" mass="12500" checksum="D6814350BA717E47" modified="2022-10-12" version="1">MRLYIILSLYFLLTKEQMTYEECKRDCTMDWLDCVSGCDSIGNPSFCHKNCQNVVHSCLDECPKIICIDDCRINLEQCRKNGKGVCEILFNHCELNCQDTYNQKEDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VFJ3</accession>
    <name>A0A8S1VFJ3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BART domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0650157</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000065">
        <property type="protein sequence ID" value="CAD8176588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VFJ3"/>
    <dbReference type="OrthoDB" id="307461at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097546">
        <property type="term" value="C:ciliary base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023379">
        <property type="entry name" value="BART_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038888">
        <property type="entry name" value="CFAP36"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21532:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21532">
        <property type="entry name" value="PHOSPHODIESTERASE HL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11527">
        <property type="entry name" value="ARL2_Bind_BART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BART" evidence="2">
        <location>
            <begin position="7"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="113"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="188"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8176588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="257" mass="30205" checksum="796395CEF6B5D778" modified="2022-10-12" version="1">MSSDYLTVKEVILQMLASPTWQNQYNSFVDEYCIYFDDDEENSIQQNNIFKQFQTEMAVVYDSFFSSLGLDNSDELQIQVIKQILNSDGEEEIDVQQLLALGDFQVFKAEMSYQNKRREIAAYQQLVDDEEEEEDDEEEQEVEQNPLAEQAQKMLILKIKLEYEQLQEVLELKQAMQISLDTPNQKKLELISQLLEVVQARLQILEKEEEGMQQKQQMTQDNDRKAKLLAQLNDLPLIDLQKEQEIFTKIQIDVDKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VHE6</accession>
    <name>A0A8S1VHE6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0640095</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8175953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VHE6"/>
    <dbReference type="OrthoDB" id="341259at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053080">
        <property type="entry name" value="PP1_regulatory_subunit_27"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46899">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46899:SF3">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="1078"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="1112"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="870"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="871"/>
            <end position="889"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8175953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1153" mass="133398" checksum="3151DB2FC01DE7EF" modified="2022-10-12" version="1">MNQNFFDSANLKQQLYKALSKYNGPTTSTPSILSEYLSSAKTQTSNNKCNSNKVKPLLSPQNINKPIQNDSLRIKTSTLHFPQKQQVSHHIKSLSTDIKQQPQPPIRRRGRQEKMIFQSDSLQAPFLIIQNKVRDTRRFDTFRKNTLPSLQTESQRSIQNTSPRITEIPLIPILQVTEIQDSKSQLFQEQEKICFDQGNFEIENQFNNENKQASEFFNSIKRKVSITEPNDLIDHQNPTEQQEEIIQTEKEVICDEDELQISNAMKKLSVKMKFKNVVSQQVQNMFKNRELKSLFTVIISPEFSSKHEEERQKISDQFEQGNFILAKNLQPNKDSYKSKQSIKYIKEKYNFKHNHKINTIVQNQLQKLHTTNKAFQRKIQMIKDTQWTTKECDQPQQQQFSLLNKFKKQSVLSNQIGLGPVLQSNLENILPNLMDDANKITITITNILLQNTILEFNKPKFIVHMDSIIDEQVIQPISPPKKAIQHSSSLHSSFKLQKIQNSASQSVIINKDHISNHNVYNSIQNTNIIEDNSNLDFNQEYSKSNLYVRLYYQNRLHKIPQKISYTQHNLKEIEDVFYQYVSMITGSQISFQASQNFTKQIKSVEISNLVQEWQIVLERYDIPQKKKQFSFDFDNSISLLSSQSDQSASFDKILEEPNQRLQVDERHESKMQNIIQSMKNLSDDSESQIFVEDVVNDQKQISTGLLNTIKKVKNKFHCPMNGQAIMSILDQNTKNDLLTFQMMEQSKEQIQKLIHGDCDTNALKNLDSFYKQQEVRKMMYHGIEMRYENILIGRYQSQQRMNDVEHSDLVQSSILEAPQQHISSSQQSQQSQQSSELLLLQKQKLISLKQQGRSRNLQRDSLTVIIKPKINSKNTSNSPDVTNVHSKPTSQYTNQLTYNIFTQASTQYLTKPTNQSQQSVKVSSVQNLEENLSNLEMSPQSSLKSIKSKSAIDSSNIISQTNPPNIRKSQNIRDFDKITKDRMNHPKEQIELKEEIISSKRQKSSNISLLQQPILENKTLSMFSYSLRMRNQALGHSRRQNLDQLKILEQLIYDNDVQEFLEHIQQVPQMYIDKRLENHDTLLTLASKSGCKEMVKELIKKGADINIQNQDDGNTAVHLALSYGHYKIADILIGAGANTNILNNIGKNAWSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VHJ1</accession>
    <name>A0A8S1VHJ1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">20 kDa chaperonin, chloroplastic</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Chaperonin 10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Protein Cpn21</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0650201</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GroES chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000065">
        <property type="protein sequence ID" value="CAD8176674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VHJ1"/>
    <dbReference type="OrthoDB" id="184876at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00320">
        <property type="entry name" value="cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.33.40:FF:000001">
        <property type="entry name" value="10 kDa chaperonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020818">
        <property type="entry name" value="Chaperonin_GroES"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018369">
        <property type="entry name" value="Chaprnonin_Cpn10_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772">
        <property type="entry name" value="10 KDA HEAT SHOCK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772:SF0">
        <property type="entry name" value="10 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00166">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00883">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00681">
        <property type="entry name" value="CHAPERONINS_CPN10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00031971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00073031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00079398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8176674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="99" mass="10975" checksum="929CB71D4B1B8868" modified="2022-10-12" version="1">MQQFKRLVPLMNRVLIKKLEVPNKTQSGILLNTGDTKNPAGVVIEAGEGYYDHKGDFVKICVKIGDTVLLPEFGGQKVNVSGQELLIFRDTDFLGILSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VHV6</accession>
    <name>A0A8S1VHV6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0620147</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CFAP144 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8175052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VHV6"/>
    <dbReference type="OrthoDB" id="283491at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097546">
        <property type="term" value="C:ciliary base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029214">
        <property type="entry name" value="CFAP144"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33865">
        <property type="entry name" value="PROTEIN FAM183B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33865:SF3">
        <property type="entry name" value="PROTEIN FAM183B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14886">
        <property type="entry name" value="FAM183"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8175052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="162" mass="19017" checksum="B589BBB70517CFC0" modified="2022-10-12" version="1">MAKQESGGKNNKRDRSPVAEMKIWQEAVRKENQHLKVYEHFTINPHKLYIIQEKPNNSIMLQKHLEKTGAVQKPAFDVNQITDDSPPLEKDIIDKLNTMNRTPRQKYQFPQTSNQELGWHSINSNSLSPSKFTYPRKLCKETNYANDYFTMNKISPYSNKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VI18</accession>
    <name>A0A8S1VI18_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphoenolpyruvate phosphomutase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0640211</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8176183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VI18"/>
    <dbReference type="OrthoDB" id="429143at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050188">
        <property type="term" value="F:phosphoenolpyruvate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00377">
        <property type="entry name" value="ICL_PEPM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039556">
        <property type="entry name" value="ICL/PEPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012698">
        <property type="entry name" value="PEnolPyrv_PMutase_core"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02320">
        <property type="entry name" value="PEP_mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42905">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42905:SF7">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE PHOSPHOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13714">
        <property type="entry name" value="PEP_mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8176183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="298" mass="34056" checksum="FC117889FCE46187" modified="2022-10-12" version="1">MFKFLLRPSLLFSKRTTKLKNLIQSKELEFIMEAHNGLSALIVQEAGFKGIWASGLSMSAQLGVRDCNEASWTQLLEVLEFMAERTTIPILMDGDTGFGNYNNARRLVRKLEERGIAGVCFEDKIFPKRNSLGDGAQELANIQEFSNKIKACKDYQRDKDFQIVARCESFIAGWGLENALERCEAYRKAGVDAVLIHSKKSDFTEIEQFLKAWNNRLPVVLVPTNYYKTPTEEFRKNNVSLAIWANHNLRASIKAMQDTSKQIFERQNLIGLDNIASVKEVFRLQNEKGLEVDDKKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VJN8</accession>
    <name>A0A8S1VJN8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">40S ribosomal protein S14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0670222</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000067">
        <property type="protein sequence ID" value="CAD8177464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VJN8"/>
    <dbReference type="OrthoDB" id="308517at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.80:FF:000002">
        <property type="entry name" value="40S ribosomal protein S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_uS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_uS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018102">
        <property type="entry name" value="Ribosomal_uS11_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007176">
        <property type="entry name" value="PRK09607.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S14/30S RIBOSOMAL PROTEIN S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002131">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00054">
        <property type="entry name" value="RIBOSOMAL_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8177464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="168" mass="18140" checksum="A3EADAFCCFC022F8" modified="2022-10-12" version="1">MADNKVQPAAQQAKGKKGKKNQGDDQPKEVVATLGPNVAGNELVFGVAHILSTWNDTFIHITDLTGRETLARVTGGMKVKSDREESSPYAAMQAAKDVYEKLKTLKINALHIKLRARGGVDTRQPGPGAQAALRALARLGLKIGRIEDVTPIPTDSTRRPGGRRGRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VL92</accession>
    <name>A0A8S1VL92_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-directed RNA polymerase RBP11-like dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0650019</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000065">
        <property type="protein sequence ID" value="CAD8176312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VL92"/>
    <dbReference type="OrthoDB" id="510325at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07029">
        <property type="entry name" value="RNAP_I_III_AC19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.10:FF:000018">
        <property type="entry name" value="DNA-directed RNA polymerase I/III subunit, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00261">
        <property type="entry name" value="RNApol_arch_Rpo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009025">
        <property type="entry name" value="RBP11-like_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008193">
        <property type="entry name" value="RNA_pol_Rpb11_13-16kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033898">
        <property type="entry name" value="RNAP_AC19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022905">
        <property type="entry name" value="Rpo11-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I,II,III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946:SF28">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13656">
        <property type="entry name" value="RNA_pol_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01154">
        <property type="entry name" value="RNA_POL_L_13KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RBP11-like dimerisation" evidence="3">
        <location>
            <begin position="43"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8176312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="122" mass="14005" checksum="D78B9D3349632C49" modified="2022-10-12" version="1">MNSSESEDQDINIEEEELSKPILQVQSAKGMEIQENSDPFNGTFVFFDEDHTLGNSLRYILANQPNVEFCGYSLPHPSENKMNLRLQTMNETKEKVMNDGLKCLSQICDIIEDKFKQALEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VLT5</accession>
    <name>A0A8S1VLT5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0690034</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VLT5"/>
    <dbReference type="OrthoDB" id="306754at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8178190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="491" mass="59348" checksum="19FC81E45AC45486" modified="2022-10-12" version="1">MDQITLIWNKFRNNIQNVFKSKNDDIDKRITLILNFLIKEVNLNHKRNDHKIENLVFVRAFQINIGLIQCEYCFLQNKNNLNYQYSLNLIQFLEDVFYFIDQISSLFSNDQQYQEEKNYHQLRIYNVMKETFDTKSINKDIIWKINKFIMEIESALFYPEDFIDYLGNSHYQRINRIQINQIIQNIKDTQQQIIPYRSYINLLPKVTYLKKFHSQSDYIYQQTISTQFLAILGTNSITIYDIKSGIKFSTINVGYQNNNIQLFQFTKDQNYLIFAQDKPKYYLIYNMKEKQIQQMQNSQASFIECINYNNQNYLFFYDQNFKMNKYLLIEQQSFFDCKIFKQFSYLDNSTKVVTFYNQIERKWKNLTKHFLGGRNTKNIQITKKFKLLQIRASRMNHIYYTLISNSNKLIRRISSQDYFIKDIYFISQDESYIISSKLTFYEISTGKIAFEIRLDVGEEVLDVKVDDGKIAILTWDNLYIYDEINKKQQKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VS12</accession>
    <name>A0A8S1VS12_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="15">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPENT_87.1.T0740100</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Membrane</location>
            <topology evidence="15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="15"/>
    <dbReference type="EMBL" id="CAJJDO010000074">
        <property type="protein sequence ID" value="CAD8180394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VS12"/>
    <dbReference type="OrthoDB" id="3352408at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02081">
        <property type="entry name" value="P-type_ATPase_Ca_PMCA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000039">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000045">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000191">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006408">
        <property type="entry name" value="P-type_ATPase_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01517">
        <property type="entry name" value="ATPase-IIB_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF369">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 15" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 15" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="12 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="124"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="290"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="337"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="775"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="808"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="847"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="889"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="929"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="959"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="16">
        <location>
            <begin position="44"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8180394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1042" mass="116644" checksum="086190345092CEA0" modified="2022-10-12" version="1">MSRVNLIEKNSDSELVRQGEDGDFKNLFILDNIRDGVSLGLVQQLGGEQGLAKIFQVDLKRGVEDEEQVITLRNRYGANLPIVKELTPLWKLIVECLGDTMLQILIVAAIVSTILGIIENEGGWYEGLTIFLAIFLIIGITAGNNYAKERQFAKLQSKLDEGHVQVKRGGNIITINNKDIVVGDVLLFQLGDIFNVDGLYLHGSEVKIDESAMTGESDEMQKASVEVCLKDQKGKSPFLMSGTKVNEGTGIMLVLQVGYKTVQNEMKRLGESDNTPTPLQVKLEAVAETIGKVGVVVAILTFIILLVRLFIEYGTNDNLTFWEQFWHIDCLQKILKFFMIGVTIIVVAVPEGLPLAVTITLAFSVNKMKDEQNLVKTLASCEIMGGVNNICSDKTGTLTLNTMQVNTFFCQGVNYKDYQLPQIKNLQKDYLDLLAASNLYNSSAYPKKGINGKFEQIGNKTECALIEFCDMLGYQLSSYRPSDNILRVIPLNSKRKMMISIVHHNDKIYLFSKGAPEMVLKKCSKFINSNGEEVKLTSQDINNMLQIIEDYASQALRTLGNAYKLLNYHLEYDFDSIPEEYLLNDLTLLNIAGIKDPVRPDVPSAIQQCYRSGIIVRMVTGDNINTAKAIARDCKILGPDNELHEYEAMEGSQFRQLTGGLNKVIKDGVEVQEVKNLQNFQEIAVHLKVLARATPEDKFILATGLKQLDNVIAVTGDGTNDAPALRKADVGFAMGITGTDVCKDAADIILLDDNFSSIITACKWGRNIYNCIRKFIQFQLTVNVVALFMSVLGAAVTKEAPLTSIQMLWVNLIMDTFASLALATEPPSDRLLNRKPYGKRESIVNSIMYRTVIGASIYQILILCLILFISNSVFDFDESLDVEYEGRPIQRLTMFFQTFVLMQICNSISCRKLDEVSLNPFSGLFNNSLFWLINLIEVGVQYLLILFGDKFAVVCDLTAWQHIFCWIFALGGMIVAIFVRTLPSKWFNGINIFAEEEIEEEKLDETIASKLRRKISVRIGSVLDDNHENKRSIQKRLSIYKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VUL2</accession>
    <name>A0A8S1VUL2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CFA20 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0760196</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000076">
        <property type="protein sequence ID" value="CAD8181548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VUL2"/>
    <dbReference type="OrthoDB" id="303411at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="473"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="473"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="501"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8181548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="526" mass="60543" checksum="B8EAB5CA54271D64" modified="2022-10-12" version="1">MTHLPKINQENTLFDCRELKSQQSENVIFDKTTKSFVLKVGPSNLFTYHNSDDVYIKGSKIYIQIYLIEKLKFILELSNKQEYFRIVVSPNLQQAYSKKLEEVKISSAFILTNKWISLSFDIESFFKDVELQFKMLSIHSLCRLKKIYEGNQLNNINDGHCLIQKICEKRILELLPNSIRAQSEKTDIRKNQLGSQQQRRLSTISNSQEKIVQTESDSKKYSIKQKNLNFQELPKNVHLGLKTKSQTQPLNQNLKKQQVSLHQKQLSNQKNQIQTKSLSQQQPLQSLQIHNQGLNQKGQLSQISSNRSKKLTQIGGNLTKQLSQECLLEKTIKLATGLQVIEEVNINKPPVYAKSSSNKFTAQTQLQKKAVQSFLRHTQEFQNGSHFSHEPFDDSKTDNELGYKYSQVSQGVKDLYYEGDNSEYQTKYGKFSSTFTRPLGLSSYNHRLSSQQSQNQQQGSTNEQFYLSSQQVEQNFSPNQTANEIEEQIDAPEIKSKMTDDSFNSINGLQIKQEDSSEDLAVSKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VV21</accession>
    <name>A0A8S1VV21_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0690026</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VV21"/>
    <dbReference type="OrthoDB" id="10263264at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8178176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="498" mass="59483" checksum="51FB6C94911B5918" modified="2022-10-12" version="1">MLLHYAIHIVEKNQSINDSSTMTNLDFRLIKPNTGEKYYYPVFGKNCNHFVEKFIDLQDVVNGFNSQNNSYKCPHCAKVYEEVSDFVTHDFYIRIKNDFHPLIDKISVLHGILLSHIKRNKRRFGDPLTSNQIQQKIKFLLTENNEDQFEKAQQFSYLAFFDQKLKSNNQFQFWSICLLDNVNIKIPVRHKNCKHIENYRDLQNKQLQERESNQYLKCIKIGCSSLFPLISDQKLLDCLVVDFELLKTIKRSYPGNQLFVVSKLNNDEITLSDYISKSVSNKDQFISIYCNNNKLDKLFQLSFSEFQRIVLEKMATVINLELHRNVQEKINQYKHSNCQKKLKYQSGQMVELLARCVNCPIPKENESQNSETKISLLNWDIRTFVAFIVHKQNKKKIRKKLLKNYNALYVKLNLEGIWFKFNLTMNQFFMILSYIRKCRLTQKKLEISLFSYKGKEYLLENFKNLWKYTREDFQVIKEIVKFKRYIMNQFIIQILKIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W255</accession>
    <name>A0A8S1W255_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0780074</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W255"/>
    <dbReference type="OrthoDB" id="288275at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8182199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="125" mass="14814" checksum="FC3DE79C840CCE95" modified="2022-10-12" version="1">MKQNGERTIERTNQKKIIRVYKIQNGFISEQLIIKGKIAKMRQVFIILILASICFAQSQLRGRQQFEKQDVSLDEYSFMQFHYHTPPMDLMMVNHELEIQRMMGVPGVVMMNYVIEESPYNPISW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W2U2</accession>
    <name>A0A8S1W2U2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0800162</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W2U2"/>
    <dbReference type="OrthoDB" id="421226at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="171"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="274"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="313"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="337"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="361"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="6">
        <location>
            <begin position="471"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8183263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="761" mass="90465" checksum="30197B2FD7519A94" modified="2022-10-12" version="1">MWDSRQKNNQDLLVVNELSEYSSVSLKKTLLQGSQNQIEMMPLKPKPVQIVPLSEEYAYTMTQHIQLIGDLAAIDKAAVCTQNWKDTVRKQFNTQTITQFKTSYQINREIQLLTQLKRTYVNLMPIIDPQSSFLSYWKIPVLIITFIVFIEVPLIIFFGDDFYYTFHSATYIFFRSVVILIFMMDIFIEFHVAFYKYGALVKNRRRVALHYLMGSFTFDFVPLIEILITTFSYTMANVYTFHLLFLLKMYPVYEIDQRYQDKLQVYPKWKSIYMIVRMIIEIVYLTHFFGCIFFGTGMYMLNHYDNPDEGKYTNSWLTFSSGNFGTILYYDWTKRYILSTYWAFATLTTVAYGDITPMNEFEIIVAEVAMIAAVFLVAFNVNNIQQTFVDYFDEKRKYNKEIVAITKFMRSKNVSEKTEKEIRNFLEHYFKEKRNRDQYLEDQVFQKLAPQLKNRLMYEAYQPYLYQVPWITNNFSDDLMRELSQVMEEVDYSKKENIFLDGDANRDDNALYFVQIGHIEIFVNVPETEKQEVIMAHLKSGARFGDYAFVTGLQRKASARSKKYSQVVRLSRTDFMDKLKLFPLDHQRYCFLRDEIIFNNAYYELGIECFTCGSTDHMSIDCSLTHLSIGKEIIKSVRKSIIEKKLKKLTLNIQQNRNYQFQRNKKQKIRIIKTKQPEQAELDEYNSIINKKIQFEAFYSENQIIIESYTIDKIDNFVNYYPNFNIDFLISKNRKQEKLVFAREVRQRSVSQIIRKSEAAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W2V4</accession>
    <name>A0A8S1W2V4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Histidine kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0830004</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000083">
        <property type="protein sequence ID" value="CAD8184324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W2V4"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="304"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="640"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="694"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8184324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="759" mass="87562" checksum="3A38BAFE8FB7C3CE" modified="2022-10-12" version="1">MINHKTSAKQGLYILQIVYAIIECTRASLFSLILLIICICISLFQIILLARNRNLNIYAQLAINVMSVVAKQSDQFLLLQTFVGFALFLQNEQKRWVVNILTCLIQVTIICFYSQNNEVQSYFMSIFTIIFVTYFYWAIKLPGLKKQSSGTEFLPLHQQNSIKSIIQVDEMPQKSQNIIILEYQTLDIKQISNSLKDLLFYDEISEFNLLDSIKIIKVDKQTQRNLNQKIKGITLRQLINKCQILQKTDDILIIKTNVLKLEYHQIKLIFSKEVITLQFEDIKEFAKYIKKNSCFALMNKLFQSFSHEFGTLLNQIALISQNGKIQNPSMKEQFDFIYNCCVIMNNMVKDLKDFHQLRSKTFQLEIVDVNIDGLFNELQHLFKQQAVAKQISMFMINKVKIGFKQDEQRIKQILQNLIQNAIKYTNQDGAVYVTAEKYDDKRIKFSVLDSGIGISESVTQNIKQMLSNDLILTSKISEGTAGLGLGLLNSNYLTKQLSSFKQANKEFLQFSSKQGEGTNFWFYVWKYNLLESPSNQFENKSIQILEKKVFLHQKTNNFPPPPSLSNSKSCPSQFKRMSQKKHSYTNSSNQVFFPADAQEDNAIVIEEESHTIEDDPKPNHLPDGRIKFPSLNYPECQCCKLLLVDDEPANLFPLKIMIKILGYETDLANSGIQAINLVQMRNNSHCQYRLILMDINMPQMGGLETTRRIKLYNPEIVIVACSAFSDMQTKYEAQSAGMQDYLEKPINKESLLEILTTYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W323</accession>
    <name>A0A8S1W323_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Septin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0740167</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000074">
        <property type="protein sequence ID" value="CAD8180526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W323"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003047">
        <property type="entry name" value="Septin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="4">
        <location>
            <begin position="105"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8180526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="393" mass="46330" checksum="F0DCC36ECC113AFB" modified="2022-10-12" version="1">MQDFKRTKLESSYNSQRTEDFSIINGQPSVYKQQEQKITQFDEQASEISEYQENKSTSLKQPLPIIQQNIEKSEYESQIYESIADSQPGSEIKALIEQKKIQDQKPIYLNIMVVGQSGLGKSTFIDVLLKKKLGTGQIIRDSTLQIQEIQGQIYNNDLTLNIKFIDTPGFRHQYGLRSWLKLLCTYIHNQFNTYKTRQTQQYESKEKFQKLSQQDLDERVHVCLYFFSGPRIQAEDLQALKKISGLVNVIPILAKGDSYTKNEIIQLKQQFNDLINEYHIDLFKCQCNNSYQEKSQFGMTPPYVIISSIEQFQVGNHFIYGRKFPWGICDIFNPQHSDLSIIYKLLIGHYCFELINLTEYLYNKYEQKEKEKLKLQQQNQNWLRNLLRFFFNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W779</accession>
    <name>A0A8S1W779_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0830219</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000083">
        <property type="protein sequence ID" value="CAD8184737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W779"/>
    <dbReference type="OrthoDB" id="294251at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF661">
        <property type="entry name" value="GH16847P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="1">
        <location>
            <begin position="1"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8184737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="315" mass="37721" checksum="BF3F89CF043AFE79" modified="2022-10-12" version="1">MNIYSERAIKWMKVLGYLPSDGINQFLGEMEHKYNQYIMDITKSDEMLIFEQLSKYTLDNQAQTKQQFEEQKIKTVSSYMNIEQYQKNEQVYRMIEIDNNRILSKCEFLNNSCNYKIEQLFYTDNDFYKQNYNGVQNSTKLDLLKRILYIYSLLHPNIGYISGMNYILAPIIKVIQKEADCYFCFELVMEKQKHLFLQPDNEKGLQAIMNRFDELINNQESQIFKHINQLGFVPSHFFVGLFITLFTSYLDIEIVIELWDNMLAQEYQDYQFKIILEMLRVLRDKILKSNINQFLELITLKNENQVNIIKSILNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W7K2</accession>
    <name>A0A8S1W7K2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0840034</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8184843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W7K2"/>
    <dbReference type="OrthoDB" id="10255247at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035253">
        <property type="term" value="C:ciliary rootlet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097542">
        <property type="term" value="C:ciliary tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036158">
        <property type="term" value="P:outer dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033192">
        <property type="entry name" value="ODAD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46518">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46518:SF1">
        <property type="entry name" value="OUTER DYNEIN ARM-DOCKING COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="98"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="207"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="322"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8184843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="524" mass="62096" checksum="B603FA4ACFFD2343" modified="2022-10-12" version="1">MSVVELPDIRLYSHKSRTRSKRSNLDQYRSLAVKKSVDKMIKYNSMSSVLGQDNDYYPKQLEDQKKIDEAQEVHANLTELKKSCDNMKMSGNNKDKDIDKMRKDIDQMNYQIEQYNLRQKDLEKQENEYSQKLQEQQKLLSQSNYDKKIFEHMLTRMKKDQVTYQLRANQYERHLKQALSTCQSTSLKAQQIQVLHQKTMLALKEVADGIKQQNKNREKNINRFKNELKQKQDVEQKRDERIKRQQEIAEVAANDIRDGALKKWRKLLLVHKFLNSFLKSKMQIGINQYQNLEVAYQKIKASTGISDANEIVQKFIGREQTYAHLLISISDYEKKINNLKQENQELKNNFNHLKQDYSDMDKEFLVENHKQRNEQTDQDKMVIEVEEKATISGLLQNKLNNWIVKNLKKLAQTKDKGFQGIIDAIKEKLLNLSAMQQQELLNKSIRSSVLELNDQEFLKRNFRIKPKKQNSHLHSNNSQDYSILRDDDELFNELPSKDEEQEDQEEDHLLNQLREEVKGKLLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WD99</accession>
    <name>A0A8S1WD99_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0870149</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000087">
        <property type="protein sequence ID" value="CAD8186585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WD99"/>
    <dbReference type="OrthoDB" id="300214at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8186585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="93" mass="11206" checksum="6D0392C3ACAA490C" modified="2022-10-12" version="1">MFLIKPQTPTFGDKKQSRKPQLQINEINNNSRFYSVPTRQTQQQSRSSSIRKKNDLLIKKWKELYEEQTSYYMRLLKVNGIQNIELPKFQLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WER8</accession>
    <name>A0A8S1WER8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="14">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PPENT_87.1.T0880178</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 14">Membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="14"/>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8187162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WER8"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF218">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 13 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="262"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="317"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="863"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="896"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="952"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="985"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1013"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1055"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="16">
        <location>
            <begin position="10"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="15">
        <location>
            <begin position="102"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="17">
        <location>
            <begin position="832"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="11">
        <location>
            <position position="383"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="383"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="383"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="384"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="385"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="385"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="457"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="498"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="522"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="558"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="641"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="642"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="643"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="788"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="794"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="814"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="817"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="818"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="818"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAD8187162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1141" mass="133221" checksum="32E40CC11272AEF1" modified="2022-10-12" version="1">MQQESRKIIINRRDQNILFPSNAIKTSKYNMLNFFPKAILLQFMRIANIYFLVTAVLQSMPLISPLAPFSAISPLVFVLAVSLFREMLEDYRKHRNDDMINEQVALLYNNYVFQKIQWKEIRVGDIVQVLEDFTIPADLCILRTSDENGQCFLETSNLDGERNLKTKYAITEIQEQMIHGKFSDLTGELQCDKPNNRIHKFQGMLQVDLKQYPISNNNILLRGTTIKNTKWVVGLVVYTGHDCKIIKSQGKMRYKSTHIERALNIIVIVILILQAGACIALSFFTAYNYNPLNFEGKPQFIYIYPDNDKNGPVVTAVISYFSNFLLLNSMVPISLIISLETLKYLQTTWMEFDEQMQSENQPFRVLNTMIHEELGKIEYVFTDKTGTLTSNNMEFRQCCIKGIAYSNEDLLEIFKCDNIMNDLELDKYINFFLCIIICHNVIVDEKLNEFQGSSPDEVALVKAAFNHGFKFLKRTNNSIFIKVREQLLVYEYICEFEFTSDRKRMSMIVKDMQTEQLLLFCKGADNIIWRRLDMRKHQEQELQMSQVSLKKYSKEGLRTLCLTYKQLDEIQFQDWQKQYRSIQNEVALDPEAINKLKEHESILEHELMLLGITALEDKLQEDVPEVIKSLHDAGIKVWMLTGDKMETAENIGHLCHLIDQSTKCFRVNQEDQESILMRFKKIGRSIQTYEKITKKTNKVKKNQLLQQQLQWLREQSSINSQIRSVHKMKINQMSVKSEGSDNHSQKEIKRTFALIVEGDSIYYLLHSKQIQEEFLKIIPKCRTVICCRSTPNQKAEIVGLVKKNLKSITLAIGDGGNDVRVGILGKEGNQAAMNSDYFFCQFKHLWRLLFVHGRWNLYRTSYFVNYFFFKNILFTFQQFYFAFFNAFSSQSFYEDGYLLNFNTLITAVAPVYYGVFDQDLDVQDTYIKSQLPRVYSEFKYHKLFGRRAFAKWYVSGLLCSALVFFVTKQIYLIQVNSESGRVDGLWQLSISCYWSSVFIVFIVIISDSNQFTWITFIAYGPLSILAFFPVFTLAWNEFQSETKHYAYDWLGNAKFFIVIILILGTVFIFKRVVNIWQQLEYPTIIDKVRKEKQELDQVVEQSLPPGSSRQELSELEFPISIENQVQGVYNLSHNMGQQRDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WFI8</accession>
    <name>A0A8S1WFI8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0900194</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8188073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WFI8"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053083">
        <property type="entry name" value="TF_kinase-domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44305:SF2">
        <property type="entry name" value="SI:DKEY-192D15.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44305">
        <property type="entry name" value="SI:DKEY-192D15.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="17"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8188073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="313" mass="36742" checksum="F3E823A6B860491D" modified="2022-10-12" version="1">MGNALDNEKSQQIMKDFTFCQNLSHRQLGNIRILEYKQNKKIRVFEIQMGTTSKQEIESYCKRFENRKQWKNDNLVRLLHFHENSNSMMCADVSQAIIIGEYYQMTLNSEAEQKLWLITYQIVSACAFLEEKGLYHGDLKTQTIYLDESENIKLIEHSFLPNHPDAYSKAFILDEFHLLPPEQLEELKQSIPSSQMSGVKSDVFTLGLILLELAAQQSSSAYYNWQKKVIDKEQVQRMCDMLLCLGYSSIFQKLILQMISEYEIRPTFKIIRDGLQNLEKDILGGIQFYKNHIKQERMKEANKKQFQTFGQKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WLQ4</accession>
    <name>A0A8S1WLQ4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Histone-binding protein RBBP4-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0940083</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WLQ4"/>
    <dbReference type="OrthoDB" id="427795at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022052">
        <property type="entry name" value="Histone-bd_RBBP4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050459">
        <property type="entry name" value="WD_repeat_RBAP46/RBAP48/MSI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22850">
        <property type="entry name" value="WD40 REPEAT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12265">
        <property type="entry name" value="CAF1C_H4-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Histone-binding protein RBBP4-like N-terminal" evidence="8">
        <location>
            <begin position="20"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="175"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="263"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="307"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="92"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8189551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="405" mass="46708" checksum="6DD8995377E55AF8" modified="2022-10-12" version="1">MAANQDQIFDEQSQEATINEEYKIWKKNAPFLYDLAISHPVEWPSLTVQWLPKKETPPGQDYSIHKLIIGTNTGDNEMNSLMIAKVRLPRDSDVQQDPSEYKQNEPSGIGKATGESRIEIDVRINHEGEVNRARYMPQKSNIIATFTTKGEIHIFDYIKHPSQPSNNLVKPDLKLVGHQKEGFGMSWSEQKLGHLLTGDYDGKLCIWDVETNAPEPKQTFQANNLQIEDVCWHRYHPDIFGSCGDDRHVRIWDTRKPQPLSDIQTHAGDIYCLDFNPFNEYFFITGSEDKRINLFDMRNTEKPLHTFESHGDQILSLKWSPHNMKIFVSSSADRRCMIWDFGRCGRSQTPEEAQDGPPELLFVHGGHRSKVSDLDWNLNEKYIISSVEDNNILQVWQLGAHIYQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WNV7</accession>
    <name>A0A8S1WNV7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0990169</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000099">
        <property type="protein sequence ID" value="CAD8191698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WNV7"/>
    <dbReference type="OrthoDB" id="304821at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332:SF175">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="836"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="930"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="975"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1003"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1036"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1094"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1119"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1184"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="8"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="53"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="101"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="142"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="196"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="304"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="338"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="372"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="417"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="459"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="568"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="603"/>
            <end position="648"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8191698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1243" mass="142586" checksum="BEBB3BA8CB6164B4" modified="2022-10-12" version="1">MDQINVSLCEYNGNNCIDCIPTFTLSQSINYKCECSSGYYQFDSQTCLQCVSPCQTCEQNQNHCLSCVDSNQIVNSSHFCVCQTGWILNTDGITCIKCQLPCLDCVDTTNKCITCQDSIHQLPESCQCESGWILDDNYFCIPCQQPCKTCEISTNKCLTCLDTNHELNNIQQCVCKSTYYSDSLITCAKCQEPCNECDINGCINCIDINQVLDSNKQCICKSGYLQQGILCLQCQIPCSTCMNTVNECLTCMDPNQIMKDQKCLCKEGFVNKGNFCCDQYCIDCQGIDICNNCMKGFYLSTVSRCLKCIENCDVCYNQINCENCQNGYFINESRKCETCIPSCNICHNSFSCDECLDGYYLVNQKCEPCNQNCQTCNEQSEKCLTCRSNYQINSSNQCVCKVGYYEQQNQCYRCEYPCKKCLSQAICQECFLLSNLQLDLNFQCNCNSGYFWNKKNCSQCNQSCLTCVENQYYCLSCDQRLHRILDNNRCLCGQNYFESENGICISCQEQLGKSQESCKYQDCKDQIWTYGEECDDGNDVNRDGCSYCKIDHNYSCSNEILKQSICFQCSTNCIKCQLNSLTNKSSCIQCKVGYFLDKNDCVECSINCLECIDQAYNCISCKFPQQKNHKCQLCESGYYADEINGRCLNKCGDYIKVKEEECDDGNLIKDDGCDDQCKLENKYIFLNGVSILPNYPKPQLQIVGTSQIYSATRLFKLSYTTPIVVTDDFQIRDYLSFHIQNNHNVSKMDQSFNLTQDTSKINEFNQSELNLIINITFSRSSQDEILLVKFLNTSVIYSNERYSQIETEVSCSIPKVTYIDDVTIAQVQIATNSNTYILYFIGAMCGGSVLFGGIEVFFNLLDTLQMLSYLKYINTQLPYNLQTYFQLFGFAQFNFIQKVFDLSGFIDQILNTQQLKKIPKKVASDDLTSLFIINAATITTVWISLFCIYAIAKIIPKILYSQKFKFYSESAAENSWLVQVGVYYLTIKYIINRLCYTIISEFFYSGILRAHMATAYDFTFSMVLQLYALELNSPNLFISFSSFFACFASAIYIIGIYYVIQLSQMKKYALNNKAIQAKYGSIFEGIKINQFSKYLNTILLMKKLIFMLLLIFAYEFPTFQTVSITLLSISMSLFYIFFNPLKDKLEYIKQLFSEISISFTLLNITILTCDFELVYFSYQIRQYLGWGCIFFMTTLLCIQLVIDGFQQWKFLFKKYKQIRRIAKSILGVFQKTRIATAPSNIFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WRZ2</accession>
    <name>A0A8S1WRZ2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0980098</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="9">Microtubule inner protein component of sperm flagellar doublet microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RIB43A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000098">
        <property type="protein sequence ID" value="CAD8191135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WRZ2"/>
    <dbReference type="OrthoDB" id="308880at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008805">
        <property type="entry name" value="RIB43A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14517:SF6">
        <property type="entry name" value="RE41410P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14517">
        <property type="entry name" value="RIB43A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05914">
        <property type="entry name" value="RIB43A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="105"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="107"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8191135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="143" mass="17425" checksum="2B3EBBC639706692" modified="2022-10-12" version="1">MKRTKETIFRQHPEQQQRQGISGLKEFDGEDLQYDARNKYNKEQQKQWIQEQIEEKRRKQEQEKAEEMAYAQQTLEINRFRGMLQDNFAARKTDIGVATKQTNLQFAKEKKDKEERDRQEKLQYEKNERDLLLERGRKQPYNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X0C4</accession>
    <name>A0A8S1X0C4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tubby C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0990168</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000099">
        <property type="protein sequence ID" value="CAD8191696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X0C4"/>
    <dbReference type="OrthoDB" id="8775810at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000007">
        <property type="entry name" value="Tubby_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517:SF7">
        <property type="entry name" value="PROTEIN KING TUBBY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517">
        <property type="entry name" value="TUBBY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01167">
        <property type="entry name" value="Tub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubby C-terminal" evidence="1">
        <location>
            <begin position="60"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8191696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="300" mass="34548" checksum="0207C993825787A3" modified="2022-10-12" version="1">MSDQIIENNDNMARQKIINDGHEFENSSDQKLPSLIIQSPLHLPLSGEEFLPTNTDQFLNSIIPQGTILECDVKIVKGWFGRHLKYNFYHSKTQKLLLSVKKSQCPRSHQFQLSKSENELDFVGTLESNFSGTEFILYSNGLSYKNSKDKASLRKELAFIHYEYQLLKTRKGNLFKAYIPSLIEGQPSQIIPTDENTGLKIKSRFLNKRNQEYFEFKTLEPVWSSKYQSYTLPFNSRVNSASVHNFLLKQLKEDGKLSKEVAIQFGKCDGKYLNIDIAYPFTPLQAFSIIISQLDNKLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X4E3</accession>
    <name>A0A8S1X4E3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1090148</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000109">
        <property type="protein sequence ID" value="CAD8195495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X4E3"/>
    <dbReference type="OrthoDB" id="10264376at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001546">
        <property type="entry name" value="Cilia and flagella associated protein 52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002123">
        <property type="entry name" value="Elongator subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:003305">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024977">
        <property type="entry name" value="Apc4-like_WD40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055439">
        <property type="entry name" value="Beta-prop_EML_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050630">
        <property type="entry name" value="WD_repeat_EMAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720:SF39">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720">
        <property type="entry name" value="WD-40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12894">
        <property type="entry name" value="ANAPC4_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23409">
        <property type="entry name" value="Beta-prop_EML"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="EML-like first beta-propeller" evidence="5">
        <location>
            <begin position="52"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="328"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphase-promoting complex subunit 4-like WD40" evidence="4">
        <location>
            <begin position="393"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="413"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="455"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="504"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="553"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="586"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8195495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="621" mass="69562" checksum="2CB4728D74180979" modified="2022-10-12" version="1">MKQPSADILEMEHAIGYSGRIPRSVILHPNGEEFICIAGASLIITDLRDAHKQSFLQGHDDQITCLAVSNRGELVASGQKGDNSDVIIWDYHRKIQKYRLSEHDYEVVCVDFSHDDRLLFSSGNPMDKRIFIWDCQTGYIVGSVNHTPEPTTIARWGGFAKDIKGRDTPKYQFATSGNKQICLWKLDAKLGVFEKEIINTGNLVRDYICMEFSKNREEYLLLGTTSGDFCVFQMKNKFFSHQIVVAALGVMSIRFVDFTRFIVGAGNGTLAMYQITEDNKINQLHKTDLGGAVNSITVRVDGLESLISTDRGLIFRFNTNNFQKGLHSENHTGSILDMSYPPGVSDRFASASEDGTIRLWDISEYFVIAKCQANVNVVPLSLVYRDEVLLSGWADGKIRMFRSDNGQQIWQIDNAHKTGVTTLCISNNLKFFCSGGGEGDVRVWEMRTREMVSHLKEHTHKVTKVKLIANETQLLTSSRDRALLQWDLKSEKRISAHIQRMGGINSFDTVPNTTVVFSTGQDRKISLWDLNQSEVQRQFETSGNPKKGDECYSICLSPNFKYAVTGGSNCSVKLWDLQTGQQISEGFGHSGPVNTVQFAPDGKQVISGAQDGVVIVWNVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X5H3</accession>
    <name>A0A8S1X5H3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1110101</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000111">
        <property type="protein sequence ID" value="CAD8196062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X5H3"/>
    <dbReference type="OrthoDB" id="289273at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8196062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="524" mass="61101" checksum="414C6AE5F941587B" modified="2022-10-12" version="1">MRILIINCYDNKDLEKFQHFQFHVMKYLAEQKELIDTENEFYVRNRDTIEDFLYEVESSYVKKEAALKFDQLDVIFIIGDCHTRPWAVHMAKILVLLRMCLRVQKLLFASGFAMQALVYLSASNIERPINLINQNGGKLSDLRELKVQIQQSDMFLENTSGDLYVFNYETSEWIPKLNVGIHLRSAAQEFQSIGKYVVKAPIYKSKQVGSFLSKNKNTETVVSIRNVYLQHWAFKDIESRFLAPLLNRWDVHNFQFNNSEKKFICLAESDEGPSIIEYPKALACMFEIDTKYPFTNTLLKNFVQYAMYNIKISNSSKPFDSIENYHKINTKSSSILDLLKNNNNQNQKKTTLELMQIRGKNGIASHSQQKLTYAQEEQKLQQMQHTLKRRSSIAHVGFTLNKNVPIKPVEINAVGKRRIKYQQGLQHQRKISADDAYLKSQRDSENDAFNEPQIIQKTQTLKTPSQSEIRKMLHPEISKSCLENKEIWIPGYLSEYQKTQGDKRISKIGGSFQSNQNTQRKRWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X5R9</accession>
    <name>A0A8S1X5R9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1050067</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000105">
        <property type="protein sequence ID" value="CAD8193836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X5R9"/>
    <dbReference type="OrthoDB" id="302785at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="342"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="154"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="350"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8193836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="491" mass="56934" checksum="78933886420B432B" modified="2022-10-12" version="1">MDIFTEQLLQQINESHVIARDSIKLIVHLGSVIVAEKYIQSQMPWIGYQWFQDQVKNLAKKHYISNDDYDDTILEDDEPNPVNIDHWRRCQGQVALVKSQEQSPIRRSETKKLTKISMGQTQSNTDIYECQIIKMGELDEDIEFDPNIESMRQAKLRQMQIQQLKQQNDKIKNQEVQEQTRQLKRLNVDSKSKYTYDFEGKVIVQKPPDIDRYPKSHQNIQEKRNLVEVRDLIPNHKLQSELITSGKRQSAYLNSMNQFAIKTTVPQIDIIVMKEGVSFYDGKSEKKKERPLPLMDIKDNNELQNTLLNQNVHMTKLEYQTITNSQQSNNNFQNKTLQLSISQPQIQQSKTSRTFQEDQPQTQNNDSLLSFRQPKTTVNHSITSNILQKLNGSISILSEEQLDGLIVQSNDINKQKEHPLFMKKPTIDISKPLQPVPTITLELPQIPPNALSNSVSKLPRSFQSIGTFPKLLSKHPRERISKQVMNIINIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X7Q7</accession>
    <name>A0A8S1X7Q7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPENT_87.1.T1140174</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000114">
        <property type="protein sequence ID" value="CAD8197215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X7Q7"/>
    <dbReference type="OrthoDB" id="285163at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000315">
        <property type="entry name" value="Calcium-dependent protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="138"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="560"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="560"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8197215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="583" mass="67025" checksum="3A74CE896E7FE4C1" modified="2022-10-12" version="1">MADQKVVSVFDFGKTRNKFWQPCDKSVLDTQSFLVEQEFLTAGKVRVKPIFIVLGLEYLYKVTVDKLKCAPLATMHLSFIDPGADHMVQLNSDEQQYGFKLTYQTKVLDIFLSEKLLYDQWKQHLRKVCLLENFHDAFMVSKLIGKGSFAKVYLATRKENNQQYAIKAFSKSFMQQQHKGIESLLNEMQVMRKLNHPNIVKLHEVHETTNSVYFVVDIVSGGELLQRVRETGFLPAETLQRLAYNLLSALNHLHQYKIAHRDLKPENLLLRSNENNYDIILADFGLAAQLTDENILFKRCGTPGFVAPEILDYMEGQQFYDEKCDVFSAGVILYLLITGGQPFTGKDQKAILKANKDCQIDFDDSLFKSAPIQMQDLIKSMLQKKPSYRLSSTECLRHPYFKELAKQQQIKQEKYQNNLTEYNQQFKNNVRAGSIDMTLEQRSPLFTGNLNSIESITCVSNNSFAKIEMKPPSVVGASKFSQYSSRLNRLGSRDISDIPNTQLQKTESKKHIDLHRIAIQNSHRKQIIDQFEDQPVHQENSEVSDMVRLYNSTRQIRIPENLSQMSQTNKQASTPTNKAKKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X7T2</accession>
    <name>A0A8S1X7T2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1140102</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000114">
        <property type="protein sequence ID" value="CAD8197079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X7T2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8197079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="227" mass="27547" checksum="3120F9FAFFCD7B97" modified="2022-10-12" version="1">MFQENIICKCHPSNSLIGKRCIQKNQSTMYIQLDNLNMKFHLSNSHQHKFGIEFQMCKFYIYHCNQYKLGYQPNILNHIQYIDFKIRTQHMELHKANINFLGCIFLEHMIYSLFHLWNMFYNQQSNHSNPESHELHYVADEQLLQLVEQIMQLVLFKQEPNWHREQLFEVVEHFRQFRSQGLQIQPSRQYPSIQDEQFEFPPSEQFTQGEVHILHVEMSKQYPAMHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X7X3</accession>
    <name>A0A8S1X7X3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">LisH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1140171</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000114">
        <property type="protein sequence ID" value="CAD8197209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X7X3"/>
    <dbReference type="OrthoDB" id="5970631at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060287">
        <property type="term" value="P:epithelial cilium movement involved in determination of left/right asymmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055289">
        <property type="entry name" value="OFD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39063:SF1">
        <property type="entry name" value="OFD1 CENTRIOLE AND CENTRIOLAR SATELLITE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39063">
        <property type="entry name" value="ORAL-FACIAL-DIGITAL SYNDROME 1 PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="176"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="370"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8197209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="446" mass="54159" checksum="AEEF816197B12FE2" modified="2022-10-12" version="1">MRQEESESLTESQVLLFMNEKMREEGMLDRIKLQVRKEIVDKLMQEPKQKKTKRLFDLKVADCFIINFLQTQNFLCTAEIFIQECGIDKVDILQTQELLQLHGLTIRDYEQHLFQLQQGTISALEIMRSVIKRKYHIEKTDHSCQTDEREETYEQKMRNLETGFLKKINYSRLHDMQTIEERMNNLRKDVEQKYQAKLEAEIVRLREHEMEMIRQEERIRYEKMLKQMKSGADEEYEEKLKELKKKEEDALIRINAKLKAIEQDKFEKQQEIQRQLHELEQEKIKYHRHKCLDSDVVKQELAEMEAIKLDLRKAIDEYKKSKESLDQQNIKQAEMLNEYWKEKIAKERQAKKEEGIDDETDWVKYQKDNCSRLQKQREYLLSENETLRQEINELNYKIQEIQKELDKCRNELMQAKDERDIEHNYSLRLEGQIRIITQINERNSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X957</accession>
    <name>A0A8S1X957_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1150118</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000115">
        <property type="protein sequence ID" value="CAD8197462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X957"/>
    <dbReference type="OrthoDB" id="308370at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="277"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8197462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="531" mass="62766" checksum="A4D6FC7B43DEEEAA" modified="2022-10-12" version="1">MQPFQHSRDDYLNKQVNKLLTKASNIHMKTCEVKVPTRRNSMIESYILTNDIQQQSDGIRKQKIDKNRSCSNLQDYQEESNSTFKQLQFSQLSIMNKNTKLIMLRVENKLKNFRFAKENDNTISTSTIAKMQSFCLDNQCFDFYSCTDLLLLKYLIYLLLQEINSLNQNYSDQQLLLSQKCVNKDTIETLQYNVDDLKNKKHRNSIENLDKQINKMQRLIGEQLSLPNYDMFTTPQSNIKKSEFVFQPSSNTQKIKCDQSQSSFNTQKMIQFQIDELKMKNQTITKLTSRINNLEEELKFVNNQISDTNLNLQISQNSLTVKDQELKDLIKKNEKLVETTNQTIQEKDRLQDQYKSVKNDYELLQTKYNELQKTNQSILKESQIQKKSLNHTVESSQQQMKKEEQKLQKELLDKNKLIRLLSEDGKILGQYLKDISYKIQQIPTDQIPQNLQMLQRELYLNECLVNTKLNNMHLAQIDLVKFNNVNNEDKKSRKFMNCHNQLIDQMKSFQIHSDLMAMMVIQSEIIEKFMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XCL3</accession>
    <name>A0A8S1XCL3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1200064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000120">
        <property type="protein sequence ID" value="CAD8198907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XCL3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8198907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="88" mass="10252" checksum="609E6DCD886BE885" modified="2022-10-12" version="1">MGFCSSKQIQSTLSSSHQSQITEVQPFPRMSWINKYLQQPQERIIKPYNPNSLEYQYNVEVEGVIFDEIAPPDFLDDDILEPIDDDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XGK0</accession>
    <name>A0A8S1XGK0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1230102</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000123">
        <property type="protein sequence ID" value="CAD8200061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XGK0"/>
    <dbReference type="OrthoDB" id="290102at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8200061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="253" mass="29924" checksum="228689C841F7F813" modified="2022-10-12" version="1">MKQLSQTSQSYKHKQKSEPSLLPNIDILKENVKSSFALGHLRGLLTYKQVRELPKTEIQMRRDQTYGKANKKSKNKRLTFLKFGFRTHEKKNNYYLQPLIKKVKDAFGEVANDGSMGVVDLWSDRNMNRLMDAIQMTRDNLIVRKLGKELNEAEHFLMIRMEEKKIRDRIFMESLKLQFQETDTFSLKFQLSDKDKKIVQRVAQPTISSNLRYNNPNENIQTIVESIDQLHQENLEIYTDLYKQIKSIQKHIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XMY1</accession>
    <name>A0A8S1XMY1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1310001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000131">
        <property type="protein sequence ID" value="CAD8202371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XMY1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8202371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="111" mass="13432" checksum="6B4321A651604FD7" modified="2022-10-12" version="1">MATFFFPLLSIASAKIKDLRKWNYFNYESKTQFWSAQCGSFQIYRILLQIVIIEEFINYPEAQSILLSMLSYMYLIYLLKFKPLQLHLKFLNQFVEDSQLCSQLEHFYLQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XTC7</accession>
    <name>A0A8S1XTC7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1380091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000138">
        <property type="protein sequence ID" value="CAD8204786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XTC7"/>
    <dbReference type="OrthoDB" id="310176at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8204786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="123" mass="14663" checksum="D94CB6F20930F12B" modified="2022-10-12" version="1">MNDYYILLYGSIPVCLPQVIGNLQYFWKNMGNYRSDKSLLCSRGMFHIRDQKKMLSSKFTSLQNDLELKILINKLLYDAKWNFITLCLRFDIKEMIQINIKKGKSIQTIPIQLISTFFLIWKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XVD2</accession>
    <name>A0A8S1XVD2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1390098</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000139">
        <property type="protein sequence ID" value="CAD8205023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XVD2"/>
    <dbReference type="OrthoDB" id="306616at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8205023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="396" mass="46020" checksum="36472033731B5315" modified="2022-10-12" version="1">MQQPTEELDQFIQTLNNQSTIPYLQQALTQDQLLAQNARLLQQLHEQQRMLNFQSLFTQSVLQNPFINQQITHESKKIKKTKSTLQRIQSNASIKEQTPQIQEIKGNVKNAYIKKITSLLSVEGEKLLDEELQEDLQGDSSSEEVSKEQKFGLAGLLGSTIDLLSAESKNKRLRQSAKNSRLRKKVYLKLLEKKVSDLDQKIQEYKKTTKQSFEYLTQLLISHPILNSMIIGNSNAIDQIIQCKQSDQAQLILDSYLMRYGTCGIKRRDYLKYAVKNIQRNFLKGNYGLQLISVNKDVNNYDDEFNNYVEIVKKYTKLDDENLIYKVLPIIDKLLNHRKTSQQLLLKFNTEMKKLKLIQQEVEENVNQFTQQLTPLQQIELIKSVEKSATFNRSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XXG4</accession>
    <name>A0A8S1XXG4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SCP2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1420122</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000142">
        <property type="protein sequence ID" value="CAD8206010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XXG4"/>
    <dbReference type="OrthoDB" id="3592703at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1050.10:FF:000001">
        <property type="entry name" value="Putative Non-specific lipid-transfer protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003033">
        <property type="entry name" value="SCP2_sterol-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10094:SF25">
        <property type="entry name" value="SCP2 STEROL-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10094">
        <property type="entry name" value="STEROL CARRIER PROTEIN 2 SCP-2 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02036">
        <property type="entry name" value="SCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP2" evidence="1">
        <location>
            <begin position="18"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8206010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="118" mass="12824" checksum="2D855D5602508A9C" modified="2022-10-12" version="1">MALKSAALFEKMDPFIKSQGTELVKKINAIYFFEVSKAKGETPEVWTVDLKNGSGSIAKGRVGTADATFTMVDDDMMAMAQGKLNPQQAFMQGKMKIKGNMAAATKFTPDLLPKDAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y009</accession>
    <name>A0A8S1Y009_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1460121</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000146">
        <property type="protein sequence ID" value="CAD8207239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y009"/>
    <dbReference type="OrthoDB" id="291213at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8207239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="50" mass="5745" checksum="CD38F1AD34E741C5" modified="2022-10-12" version="1">MIALDKILQTLDFYKNRQYQGLQIRENLEPVQLEIGLSGIASFYEQGGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y305</accession>
    <name>A0A8S1Y305_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1480010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000148">
        <property type="protein sequence ID" value="CAD8207587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y305"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8207587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="85" mass="10149" checksum="7F33531C585DB10A" modified="2022-10-12" version="1">MDLHYLEWYNLFKLLFTSMATKICRKSNMVIQFQSLQQISDPNKNMSIIISILNSHLKLRQPGQKLFKNSLFKIIIIVFKQQYIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y3D3</accession>
    <name>A0A8S1Y3D3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1450023</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000145">
        <property type="protein sequence ID" value="CAD8206722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y3D3"/>
    <dbReference type="OrthoDB" id="297440at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8206722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="90" mass="10189" checksum="658800ADA21090A1" modified="2022-10-12" version="1">MRGFFAITRAFIAPVAKQTFNFSTQQSKIQMRIVINKINHIIRLSSTMSSLRIANQYDLTLFSLLEEAQDDSTDLDNRQIGLHLGSTVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y723</accession>
    <name>A0A8S1Y723_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2,4-dienoyl-CoA reductase [(3E)-enoyl-CoA-producing]</fullName>
            <ecNumber evidence="3">1.3.1.124</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1550061</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a (2E,4E)-dienoyl-CoA + NADPH + H(+) = a 4,5-saturated-(3E)-enoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:45912"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:85101"/>
            <dbReference type="ChEBI" id="CHEBI:85493"/>
            <dbReference type="EC" id="1.3.1.124"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a (2E,4Z)-dienoyl-CoA + NADPH + H(+) = a 4,5-saturated-(3E)-enoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:61892"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:85099"/>
            <dbReference type="ChEBI" id="CHEBI:85493"/>
            <dbReference type="EC" id="1.3.1.124"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.124" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDO010000155">
        <property type="protein sequence ID" value="CAD8209599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y723"/>
    <dbReference type="OrthoDB" id="5327538at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008670">
        <property type="term" value="F:2,4-dienoyl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05369">
        <property type="entry name" value="TER_DECR_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045017">
        <property type="entry name" value="DECR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43296">
        <property type="entry name" value="PEROXISOMAL 2,4-DIENOYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43296:SF2">
        <property type="entry name" value="PEROXISOMAL 2,4-DIENOYL-COA REDUCTASE [(3E)-ENOYL-COA-PRODUCING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00026117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8209599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="280" mass="30424" checksum="586965FB0D34A647" modified="2022-10-12" version="1">MNKTFQQNFLANQVAIVTGGATGICYGISLAYLKYGCKVLITSRKEEVLKQSCITLAQESGNNNIAYFPCDVRKFEQVEAMVQFALEKWGRIDILVNGAAGNFLVPFEMMSVNAFRSVMEIDTFGTFHCCKAVVAKWMSKNGGVIINISTTLPHCGVALQSHAGTAKAGIDALTRHLAVELGPKKIRVVGIAPGAIEKSEGFKRLRMDDSSGFGEDFEKLLPLQRAGTNDDIAPWALFLASECASYITGQTIIVDGGAVNTFPNFTLLSKKARDMLQPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y7F7</accession>
    <name>A0A8S1Y7F7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1570046</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000157">
        <property type="protein sequence ID" value="CAD8209975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y7F7"/>
    <dbReference type="OrthoDB" id="6600758at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000171">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBC core" evidence="1">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8209975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="167" mass="19283" checksum="4B16E19E19AA2D21" modified="2022-10-12" version="1">MVKNRLEIEFNAVLNAIEEEILENMTVEVDKQNNSINYKKWFVTIYGKEGTIWEGGEFSGVLNFPKGYPSDPPTYQWTLYGGQFQHMNIFENGVTCIDILNNKIGYTPATTCIEILKGMEGFLYNPNPKSPTMGSLAKIYVENKQQYEKLIKAFVKNYMIEKQKKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y7W8</accession>
    <name>A0A8S1Y7W8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1550103</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000155">
        <property type="protein sequence ID" value="CAD8209671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y7W8"/>
    <dbReference type="OrthoDB" id="291245at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8209671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="198" mass="22547" checksum="92D7D5EB42C13678" modified="2022-10-12" version="1">MKKTLQVLKAQSLNYNSIRSGLNYRMTIRDNGQKMGHISINLNKYHNTQSNTILKNLLQNQEYTILRDQTKMIFEGSFDALKVLTGVTTYYPDTGLLVYDVSANKLVLHLTKENVQCQGIVLGYVMDQDAITILDSLRASTIPLQARLENTYNYYLKRTLNVSLAVSLLVFYNAFTGSQKSKFGVFERDDKSPQLNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y9X5</accession>
    <name>A0A8S1Y9X5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD-40 repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1550127</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000155">
        <property type="protein sequence ID" value="CAD8209717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y9X5"/>
    <dbReference type="OrthoDB" id="338608at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="394"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="436"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="478"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="520"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="561"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="603"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="645"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="687"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="729"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="770"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="812"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="854"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="896"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="938"/>
            <end position="972"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8209717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1057" mass="119241" checksum="3561AC6AEA27207C" modified="2022-10-12" version="1">MNCIFHKENPISLICMASHECKLQRKLCNKCQYLHGVDFKHLVSIEIFKEKVSKKLTDYQFYETHEVKQRKMKMISLLTQTESLLKQIFEELSQSIKEIYNQIEQEYKSFYEIITDTTINLAEFLKNDFDNLVQIAEGTTLNYSNVYKNPYFELLEKGKIFLDKEVKDFITKIRQEMKEIFSFNGQTQLNIQPIQKEGQGLKIEKDIVDLLASLKDIEVKIFTQIIELLKKEKISDFLRRDETQQKLNELLGNSSTNKIIEVTRNLNEHDFNKNDYSAEIYCSKIQDIIKRIGEDNTIIEFLKFLVHITAIDEQMIQCGSNAIHLLVKMKIDMTNQQFQDIKIQNTSLVGANFLRCNLSGSEFNNVDITGINLNGAQLLRCNWKNIRINQFNRLDGHSGCVRSVCFSPDGSILASCSLDKSIRLWDLKTGQEKAQLFGHYGWINQVSFSLDGKTLASCSNDKCIRLWDVMTGQKKARLEGHANTVCSVCFSPDSATLASSSKDNSIRLWDVITGQQKSKFVGHTNAVLSVCYSPDGTSLASSSEDRSVRLWDTETGKQKSKLDHEFEVNNVVFSPDGITLAAGGYDKIITIWDFKTGLQKAKLIGHKDFILQVCFSPDGAMLASASWDKSILIWDVKTAQQKAKLEGHSEGVSSISFSPDGKTLASGSLDLTIGLWDVKIALEKPNLEGHDDTINSICFSPDGSILASCSDDKSIGLWDVKTGQQKAKLHGHIYNIQSVCFSPDGKLLASGSLDETILWDVETRQQKYQLKVHTKWVQSVCFSPDGNLLASCSDDKAILLFDVKTGQQKAKLDGHTFYVQTIYFSYDGTLLVSGGVDQNIRIWDVKTGEQKAVLEGHDGCVNSVCFSPNDTILASGSADKSILLWDVKTGQKKAKLVGHIRQVRSLCFSPDATILASGSLDQDINIWDVKRQELRASLKGHYNCINSVCFSPNGAVLASCSDDMSVRLWDVKDIIEIEESDQNGQEVKVKLNLLELQKKFRVNNVTILRISKIPIFQAWGALIYKGEFFNYQGLDLRSYLKFKGSLFLENHLQELQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YAM9</accession>
    <name>A0A8S1YAM9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1600107</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000160">
        <property type="protein sequence ID" value="CAD8210750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YAM9"/>
    <dbReference type="OrthoDB" id="674604at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="227"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="272"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="316"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="47"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8210750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="481" mass="56464" checksum="72CE1CBC689729FD" modified="2022-10-12" version="1">MSSINILCPFHSKVVNFLNIDLNVKIGDRVACDECGLIRPISIVTGLEIYNRMATQQNSQIDRLLDQEVKTLEKLKNKLYKKTEELLQNIKSLEKEIEGQIQKTIQNFQNLKLTQYQIGQTTLSQLQKMAQQISLYKNQEFYQEVIDNIQNYQGVFIKQFDTALKYVEDYKKSIEELITYQKCYEHPQEICFDLQIAHKHNLIISNCENKIRSYLFKDDKLLQLQEDKSHRGYIETICLSNSQMFFFSGGVDKIIRIWKISDKGTFELSQELKGHKDCIKSIVINSQDNQLFSTGYDQTIQIWNKCESQWQQSQSLNEHKREILQLQLSQNDKILVSVSWDKSINIYQSISKVWKMIQKIENAHQYDIFSVCIINDEQFVTGSREVIIWQRNSSTGLFTQLRILQSNLGCVDRIKFHPILKIMISQNSYAVDIWRFVEVDDPLQIQRLKGLYQGSILTNDGQYLIAYNVDNKKIIINRRLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YB99</accession>
    <name>A0A8S1YB99_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T1630036</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDPK1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000163">
        <property type="protein sequence ID" value="CAD8211345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YB99"/>
    <dbReference type="OrthoDB" id="432647at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05581">
        <property type="entry name" value="STKc_PDK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000833">
        <property type="entry name" value="AGC family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000735">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039046">
        <property type="entry name" value="PDPK1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF163">
        <property type="entry name" value="3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="17"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8211345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="469" mass="55135" checksum="98865C4B2E7B4599" modified="2022-10-12" version="1">MNPDMPVHVQNLKLEDFDIIKPLGKGAYSEVVLAKNKLTGHESALKIVDKNFLFKEKKMHHACIEREVLSNLRHKGIIKLFNAFEEYDKLYYSLEVLTDGNLLEYMNRHNFNDSIIKFYAAELLLILEYLHQNGLVHRDVKPENILLTKDKHLKLIDFGTVFVYDHNKIDIGNKLEDIRIQYQKQRSPSLNDFEKPQIKYCRDSSFVGTSEYLCPELIDYNVIGPQADLWALGCFIYQLYTNKTPFYSENEFELFNNISQCSWLQDQSIPNDALDLIKILLDKDPQKRFYGEFNDSQYNYDNLKQHPFFRGINFKQMWLQDVPFLNTTNTHKRKMSRVQTTFIENNLTSRDKYDIRGISVTKHKADQIIIQGQLDKEHGVLFMTQFSLRHASIVCQDGITNFIYINPQLNNKKTVIPLNQFTSCKLLGKGKFIVGDRNKKKYIFKQKENNVPAQQWVNLINKYIKSNHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YI12</accession>
    <name>A0A8S1YI12_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1740022</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000174">
        <property type="protein sequence ID" value="CAD8213123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="294667at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="222" mass="26098" checksum="9F26B2EA122D306F" modified="2022-10-12" version="1" fragment="single">MGFVKSFYFHIDPSAFSPYQKKRCMIQILYFLMKQKKVKNIEFHYIDLYHEVTLQYLANCFQLIKVESINLLYFNDNNLAQINRIFQGINNLKIEKQTSSNLNTKWEADKIDPNASDEKINEIQFIENPKINTGWQAVQKNQFIKDDYIQLSAEYQALIPNDLRRSLNLNRGISGLIVNQLSRIEDNQQNLLVQIETNEQIIIDAKNQIDSITKRDLYAQSX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YJN7</accession>
    <name>A0A8S1YJN7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1650042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000165">
        <property type="protein sequence ID" value="CAD8211792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YJN7"/>
    <dbReference type="OrthoDB" id="10338259at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8211792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="117" mass="13423" checksum="4B52905018625C11" modified="2022-10-12" version="1">MKQYFIACRPYEKVPASLRNSLIQLVFIKGFKIKHAAKRLNIKYPAAKSIIIYHRKNVIKQKVNITQQNQCQIVPIIQSKSSITIISSVGGSQITSKILFQYEIRNDSQYILTSIPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YL72</accession>
    <name>A0A8S1YL72_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase C1A papain C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1650038</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000165">
        <property type="protein sequence ID" value="CAD8211784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YL72"/>
    <dbReference type="OrthoDB" id="640249at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02620">
        <property type="entry name" value="Peptidase_C1A_CathepsinB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000031">
        <property type="entry name" value="Cathepsin B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="7">
        <location>
            <begin position="75"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8211784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="325" mass="36120" checksum="86EF94FB09572780" modified="2022-10-12" version="1">MFIITLITSAFGLRFQSQTFYDFVNSQQSTWVSGHNERWEQFNEATLKTQMGAFLDEPDFMKLPESTVQFENLEIPENFDSREQWPGCESIKEIRDQSTCGSCWAFGAAEAMSDRLCIQTGQQIRISTEDLLTCCGITCGMGCNGGFPSGAWNYFKNKGLVTGDLYGDNSWCRPYTFPPCDHHVDDGKYGPCGESQPTPACVKSCTAQSGRNYESDKVRSVDAYSVSSKIEQIQNEIMTFGPVEASFTVYEDFLTYKSGVYQHVAGASLGGHAVKIIGWGVEKNVPYWLVVNSWNEGWGEKGLFKILRGSNHVGIEGGIYAGRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YMU8</accession>
    <name>A0A8S1YMU8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1680039</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000168">
        <property type="protein sequence ID" value="CAD8212434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="295833at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026306">
        <property type="entry name" value="RSBN1/Dpy-2/CEP530"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13354:SF11">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13354">
        <property type="entry name" value="ROUND SPERMATID BASIC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035868029" evidence="2">
        <location>
            <begin position="21"/>
            <end position="2939"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="577"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1076"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1542"/>
            <end position="1570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1972"/>
            <end position="2005"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="583"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="611"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="652"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1081"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1542"/>
            <end position="1551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1976"/>
            <end position="1989"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8212434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2939" mass="333055" checksum="47F26ADC832396D0" modified="2022-10-12" version="1">MTNKVAVLLVLVGLIGFASSSLINVQSLAQIDVESFGCDKSKLYDAEEGLQKLGEALNFAQTGKLDQDIKELRELQMLAKKGMEEQVQVSFVQVHNLQYLYMLKHKFDSLALIEMPNKFARPHIDGMKSQINLVLAATDTQTRKACCEMLFKMIEVLLLDLLELEEECQKPITINIEGEIRKIQIIRGQCDSTPTPEQPVIPVDPKPIIPDPKTPIVITPLPPKPKDPTPKPKDPTPKPKDPTPKPKDPTPKPKDPTPKPKDPTPKPPPKPKTPSPTPQVVIDCVKGANVVGDMSASSDQAVADSVIEGKDLAGATDYGYGFWLRWLSRYPDELISGRTKNTYYFVSRLTTNQKFNDAEMGDRVLAIWHYDTGYHFTALDKASRNPNVNQNIPTGDIEGLWSYVYFSYSGRLKKAVGLLKFGASGKVQQITLDVTHDIPTYLRLVVGGKQFSYPGFNGQFTNPVFKIGEGAYVNSELELFDYNLKCNPYPKLDCTERAKQTLIHKAVRXHIDGMKSQINLVLAATDTQTRKACCEMLFKMIEVLLLDLLELEEECQKPITINIEGEIRKIQIIRGQCDSTPTPEQPVIPVDPKPIIPDPKTPIVITPLPPKPKDPTPKPKDPTPKPKDPTPKPKDPTPKPKDPTPKPKDPTPKPPPKPKTPSPTPQVVIDCVKGANVVGDMSASSDQAVADSVIEGKDLAGATDYGYGFWLRWLSRYPDELISGRTKNTYYFVSRLTTNQKFNDAEMGDRVLAIWHYDTGYHFTALDKASRNPNVNQNIPTGDIEGLWSYVYFSYSGRLKKAVGLLKFGASGKVQQITLDVTHDIPTYLRLVVGGKQFSYPGFNGQFTNPVFKIGEGAYVNSELELFDYNLKCNPYPKLDCTERAKQTLIHKAVRYSVQTAKVQTVKTSLETPFPEEYSVSGWFKWKQLENQEPWHLMFRVFATKENNNAKNLGDRDLAAWIGTANDGIVLLSTYSYNNLFGEGNPNIMNQIQHKNRIQEWTFVYFGYSFKQKTAHSIVHFKEAREERTFENVNHFLVQRFSINLAKDDHYPSWNGIIGKFRIDLCNGAYDPKFPRTATPVPSPSPLGPTPPPSKTVEPPPKTPTIQPPPKPPVKPPTPDVVVSPPPPIIKTPEVMQKCIEGQAAIGDSSSAPDVKPVADIQVPEEALKDVTEYGYGFWSRWLTRHPEQLFGGMSEPWYFVSRLTSNDPYDNIRMGDRLLAIFLSKDGYTFITNDKGTSNPNLYKQQPYKDIEGVWTYIHFSFSPLKKKAVAFVHADKQTSIIELAGITHEAPKKLRFILGGNDLKLYPGFNGQFTRPVLKIGPGSFIGSPEDVQKYALSCNPLPEPDCKKGRGVSNLIESTKQFNKGSEVTMFVADNERPFPDNYAIGGWFLWTPTEQEEWHLTFRVTINGQKHNKNDQLLGDRDLTTFLGKTGGYHFATYTYTDNFGKGNPNKYINTEHQNKHTEWHYIYMAYSRQQKRVLTILFTKGDRQVKYIDEVNHYLAPLYKIFLGKDQFYPAYSGYLANVQFFACENAYDPDFKPPGEPEVPKQPEVPIKPPEPQKEPTCQKGDTNVADTAFDSQKPLIDLVLDKPEQLQDVREYGYQFWMRYMTRHPVPMYQGKRAPWYFVARLSGRKDFEDVGKGDRLLAIYQGKSDYTFVTNDHKNNNFNTDAGRFEFGDIEAVWTFVHFSYTSAGKVVSWIQYDNNEPKTFTVAATHDKPDYLEFVLGGTQGNTYPGFNGQFTSVVVKVGQGSFIETAEQFRKFAISCNPRPLPVCNKAKEVLLIEKSKRFEAQTSEVNEFASPEEQAFPDEYSISGWFKWRATVMEPWHLAIRLHQNKGMENANAAKLGDRTLGAFVGNNGNYAITTYTYTNLNGEGDPNKYVQVPYKDQLAQWHFVFLGYSRVKRQVVGFLQWKERKENINLENINHYLTNSVYVNVGKDRFYPAFNGHIGGLRLQLCSGAFTPVFPEDPKPPQVPSTPPPPKPVDPTKPEEPVQPPKPQEPEIVRKCVSGQVQIGDMPSSGDKVVDLKVTEDKLGSAIEYGYGFWTRWLTRWPDSLIKGASEPWYFISRLTNNDPYDNIRMGDRLLAIWLGQAGYTFITNDVASGNPNLNKQIPYGDIEGVWTYIHFSYKSGQAVGVVRVGDKTQFTVIDAKHDQPKFLRLIVGGSDLKQYPGFNGQISSPILKLGAGSFVNTEDDFNKFVLACNPRPEPDCKGKTESKLIEGIKKYESTQPEFYEHVRDEKSLFPTMYGIGGWYKWEPTKQEPWHLAFRVTINDQKTNKNADLLGDRTLAVWLSDQQLYAFATYTYTNMFGAGQPNAAQVIKHQDKHTEWHYITFQYNRETREAYGSIIFKDGKLDVKFPNVNHYLVPVLRILIGKDQFYPSFNGYIADVTFVNCQDPYNPQFTPGNPPELPKPPVIPVEPPQPLPEPKCQMGDETIVDTAYDDVKPLADIEATPENLKDIREYGYSYWVRYLSRHPKPMYQGKSEPWYFMSRLTVNKEYQNIEKGDRLLAIWQGQGAYTFITNDYKSNNWNLNKNIEYGDIEGVWTYIYFSYSSITNQAVGFVKYTGKEPQSVTFDVTHEKPTYLRLIIGGSDLKLYPTINGQLTRAVFKIGAGAHLDTVDELNKFALACNPQPKEVCSKAVEQVLIQESKKFELAKATVMEIPSDPSAAFPSEYSVSGWFKWKQTTMEQWHLAFRLHINKEEANKNAERLGDRTLAAWVSPAQDGIYAFTTYTYKNLNGAGDPNLAKIVPQKQQQTNWHFIFYSYNRDTRKAEGYVQFKDRKETILFENVNHFLVPQVYLNIGKDRFYPSWNGHIGKFRMNLCGGASRPVFPDDITVPPPVTPTPKPPVSETKEICLINTKLVEVTKNNIKDVIKELEALENSEPTEKTCYCTVPKKASMFIELYGEELSNHVDNPVIQRLIAQNQVRLRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YN33</accession>
    <name>A0A8S1YN33_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1710050</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000171">
        <property type="protein sequence ID" value="CAD8212832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="306787at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:004215">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:004818">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48103:SF2">
        <property type="entry name" value="MIDASIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48103">
        <property type="entry name" value="MIDASIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="431"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="1035"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="1347"/>
            <end position="1549"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="1692"/>
            <end position="1837"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="2023"/>
            <end position="2198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8212832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="3310" mass="386645" checksum="337694CFA7E11023" modified="2022-10-12" version="1">MNTIFQVQNAQQLSLSGSKLNILSKNISNSTILIFDCDFLLFREYLIISQNIQTVTCDPNLQILLIFEEQEQEQVTKLYSATLEQSFTPFQNIKIPAIKLRMVDWCNSRICALIFEKYIILYDILLNKILTNIQAEFNQINRFLISKKLTQIFCQYQEKDNNSVCLILAIENQIEVQKFNNCVSCQIIQEQEDNHFLMVQYKTNSLIIMLAKFDEEFRIEKEIKQNLSNPLQGIIKVGYQRKKQALILLDQNQNLYIYSINSNFQITQKIQNIPNIIQFNISLLNENLIILDNKNQLILKEMPAYQQIRQSQLKQSQLKQSQLSQPQTLSLASQVKKRAIQGILTDPNILKSTEHLIQQYESDFYNLSPKFKNIINLNFSYRFLPLVDFRILNLNYQENSKFKELIPKMDTYAETQSVKNHLKQLEAALKGDNPILCEGSAACGKTSIIQYLAYKNQQPLIIMNLSSFTQISDFIGKVEILPGFKFEFQLGPFAKAVQDGLWILLDEANLASDSILRVIEDVLEIGYITIYGSSINSHEDLVDGTLTIRKHQNFKLFLTQNPATDSQFAASRNIFSPSLMSQFISIKFEPMSLNDLNFIIDQIIDQKQQELNEQLVQEISTQQLSSLIMIIYEEIKQKNMDDGLFTLRDIVQMIDLYFLSVEQTKEQIFEILPLNDCMKLISTQFIYLQKYRDIINYQIDNNQLIANTNMLFVQTKDYRESYIQNWNINQPLKFLSFHKYLFPMLTLCYKTKRAALIVGQQYCGKLSSVLIWLKILNIQQYEVLELSSSTTTEDLFGKYQPTQNGFDFILGPVTRCFHQGKVLILTNFDAPDCALTESLNGILEKKFNQLLVQNQRYQRHQDFAIIALSSDFQQLEKKITPTLKSRFLSLYIDFKVNLDDITLLFQQIRQSQIIHNINQINEKLMTLGSFKTSQNLGGQIDQISLNKIIRFLKPILESEKYSKSQFNQKQSQINFECLDFFIALSLQDNKSLFNQNIAVPIHYQELLLNDGKQNNFVITESRKRVADIIAFAIIAKIPLVLQGSAGVGKTKIISTFQQTCKLFESTSFHYINMNQNTDINDLMGQFQSTCEKDKIKFILKKGPLFLGMEQGGIVLIDEANLSDASILNFLANIAKYPSEFHDPVSNQIIKVHEKFRIFFAQNPPSYNGRNQLPETLASKTIIVEVPNYTFEEVLKICTESQPSMQNQTQTSIQKAMEYFLTKLIPYPKNGDEKQLYYNGTQFSLRQFIKFRNRLEKTSFSTNQPNWENILQLHQMILFPKDISEEYSLECDINKIGLNLYFTIKNQQAQIEISLIINKNYSKYVIAHKSLNPIQRLVLCKIAFCYYFKENILIVGETSSKTYLTKLFTQLVQFQNPQPFELIYINSQTEITDLIGSMESHNLISYQQYCLNLIKKLNYGKQINFNLNEKPIQNYLQELIQKYNQPEFFNHIIQNIGNQFHFVERGLTFNARFGGVVCLKNISLAEQSVIEGLNSLLEIESHFIVNGQEIKLHNEFFVIAIMNTTFGGQLSDALQSRLTKIRIQVPQIINYTQELDISYKKYILNKFPQQTQFNQIIQYIQNLLIQTKEFKEFFYQNISDRKIYQWMSFLNLDLDDATYQQKLALGFQFVILDKFKVNFEEVVKNPDLHEHYKEAQNKIQQKLKVKQFFNSPKIIINPTTNKILQRIYSAITTQYIPCLVGPPGVGKSAIAQEFAKIMKQQFCRVCCSDSLSAEDLFGSYAPKIENNKVSFVFQEGYLAQALVQNSLILFDEINLASPEILSTLQALFNTDEKEITIKDSKYNKEKCLFLCSMNPSTYQGRQELPQCISNLLCEVYIEAFDTDQVIGIFQQRYQNEIIQLKNSGINFKIILDLHKELAILAQQQYKSNYDFNIRFLENLLQLFNQQFLFQRSKLYNNKYELELIIVCLDIIYVQHFYQTEFSNQVLNIILNKFGVSQEEWNKRKVFLEQSGNIVRISRQIGKQFKSIFNWVISNQIIPQLSNNSLVINLQNSNIFEKLLIAINSKKIILCQGDVSSGKTSCIIKMANLLNQRFILLQIHSDLETDDLLGSYALVNTNYDQIQKQLERISIKSKFGNLNVGQQQKQMNMKYVEGVLKICCKFGYWLILDNINLARAEIVERLNSLGEDQPCLYNNEFGDSNTCIIPHNNFRLICLQNPTRVDQNQLSPAFYNRCIKINFEIDLKKNFIDIIEILTIRGFGQQFIQQDTVVLANNLLKQVIFIKEHTNALINLRNIIKAFKMIQQNGFQFYDQIMEIVFGPAFNESKKNPYFPIFDLKSLNVADSIMEKYTDLILNKLVSNSVKRIVDQPNKLIDLQNISQFLKIHLSEQEIQNYQRFIQGIGQQIQIVNMQEEDQYDLGCYKIRVKGELIIQLYQQTMTIQPLFLSIYINNKELQNLLLKSKCIFQDQNILAEAKLNSEGKLQVQLQQSKDFILVPMIKSLIGPSYEQFFKTFQNMLSKSDIECSIYLDQQVEIFFNFNLSKYTLSFIPNLKLKNISGQIALNKDNIYITQFSINFDLQLELNKYFIKKGHFSRVDNLNSLRGIIKIPKEDQIYIFQDQNCFHLFSNIDIIQSHNFASFLDLKESRIEINLECENPIQIYKLPFSIRINRILANILLQQKITLNGSIQCSFHILQNNICNLTALIDKTHLKLQLSNEQHQNNCQNFIKQIFLKYYDHNNFLIQQFVESRKATVKKLDLITNALYWHFELIFELNWNINIFKNAHIEIENMNILCHIDSQTTIYTMSCTIEILKFIFEIEIVFDFESFFSQEKNRCLAMIFKRGKNSPMFKEIINAFSSDLKNQLQIISGFNQLKTIQFQRNEGSQQLFDFDNFKKQGEQLNQQFEDEVHQIKKFGCNEYKLRFENFQSDLKQKIDQELKLNQQQNMQEAIIQKLTCSQESYIIWSDQVELKFNVDLLNPWIVSKILTLCQCNLNVEGKNQIFELKLNGLIKFMNMTLNRFQLQLTPNRPLDLKIENEPLIMQIHPISFGFGLFGQQFEDIGLLPKLDQIIRFNYNIISQQFYGEFKVVKENIKQHFVDLSLENIQLQFSSSDNLKNVQAILEMKAIFFKQEINFYLQFLSNTFNESKNSSLQLKFNCHTIKNFCLNEFLKNSVGITLTQKKVEFNIENFEFLLILQKTPQWHHSILLQIKNSQCTMLQNYITLKNLNGQLVCKQQSWMDSCISGICLLKDYNIGSFNLVFNKEVNGLEAKFQLNPKFQIKDFLSTVFNQQMNFICFQNVRLDEIKVFLGLYNNKTIFELKYEVKMFSWNLKKIKQRRNQIYNLNQKFLTILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YPR8</accession>
    <name>A0A8S1YPR8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1850009</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000185">
        <property type="protein sequence ID" value="CAD8213742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YPR8"/>
    <dbReference type="OrthoDB" id="10688904at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="753" mass="88011" checksum="BB2D9A253FB678C0" modified="2022-10-12" version="1">MNSEIINGGQFDKNGLKEGWWIEFNDSITNQSQVSYYFGEYMLGKKIGRWETKIKFDNFIEFEVMQRIINNQSGGGIYDQNEIKIGQWVELVKYSGVYKKGYKSGQWYTLQKQENDFEIAGGGFYEEGLKIGKWMELSSNNDYHFTHIGEYNQGLRINRWNILFLNSDTDDYKIIGGGSYNLNGMKHGKWVEVQNKKGEFDIFAYSGEYKNGKKYGKWEILQGSITKHEYFIIGGGSFDENGFQQGLWMDIEQCSFQFDQFNYDGLFVLSIGKYQKGKKIEKWDTIFHIDRVKEAKNLGGGQFDENGIKQGQWTEIHDTFHSEKQITYFGFYKDGVKVDKWETNYMKKGQYEFMIIGGGIFDKNGLKQGKWMELHENFDNNLLVTYYGNYEFGKKIGRWDTLLLQVDESIDNSDCYSESSSQNYNSVDVSMKQTFIILSNSFQQNNLKNQNQIEEVDDQKEGIDFIQYCQKEFNIIGGGQFDKDQTKHGFWIENYFDKSLNGTIVKGNYNQGKRVGDWMSFSYNNQNKKIGGGYFDQNGIKQGQWLELRSYFKNNCLIDFGDYKDGIKIGRWDIVQQIDNTPQYSIIGGGSYDLNGQKYGEWIDVQKLSFSYSLITIFKGEYKNGKKFDNWDIKQSLKYCEKFKPICGGQFDIDGQKQGWWLEYCKNQKFKQIIFIGQYRNGLKIGKWEQMNIDEDSSNFQIRYAGLFDENGLKHGIWYLNSKSNQSNTYKVQYKKGFRISACKKNLQSISLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RZD0</accession>
    <name>A0A8S1RZD0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0020047</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8132740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RZD0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8132740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="204" mass="24459" checksum="CD5D9CC9BA28B836" modified="2022-10-12" version="1">MQKTQPVSRDSQIFETLNYKDDSIQDLDFFGPWHKQNNSIQYQIAQIKKAHTSSTNQMANEIVERPETQNSQVRMINTSFPYKRRITRKISIDQSNQFIEKPKYDESRQVERIIQEFFTDENKEKKKKQRNHGFFFPKASKSNSNTKCNGSSFQTTQEYFDVIFFFKLRENSFKNVEIYAKFIEIAIKVFKTYKKFKYKIKTDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S1E8</accession>
    <name>A0A8S1S1E8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0020334</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S1E8"/>
    <dbReference type="OrthoDB" id="295697at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="30"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8133300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="449" mass="53033" checksum="61AF23B0E107E3C5" modified="2022-10-12" version="1">MQNKDQGLFKSVAINNIVYLNLERGLVYIPNKKVGEDAGNNGEVFKGYIGYNTDKQIECCVKKFSIPTYDKQMYEKLHQELIFGRQLVHKNIVKFYECSRSDNNFYMFMEYCNGGSLAERMSLGYEDSLMSTKIFSEVEAINIIQQIIDALIYMKELSYEGKVGAVHRDIKPDNIMFQNDIPKLIDFGMARFMDKQTITHYKGSPIYMSPQNLRNEKYDLEKNDVWGIGIILFQLVEGIYPWRMELNTLEDLQKAQEKIKNNIQFKNKVSEELQGLIRHMLQYDEQDRCDWDQVTNYQIIKPYFSHQNRQQINTINRLVLNYELFEDLQTKLNIEKLGKCFLDLTLTKINNLKEFLPKNSSLEKKIDYLYIKRILQKRKFHTFKSERALKYKLSQRLVKYLEQLILDTIDQVQIKNVQLVCQIIISLYKTDVEFKDDLSLTSLLQLAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S1Q6</accession>
    <name>A0A8S1S1Q6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0020094</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GOLM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8132834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="312045at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026139">
        <property type="entry name" value="GOLM1/CASC4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15896:SF10">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_98, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15896">
        <property type="entry name" value="GOLGI PHOSPHOPROTEIN 2/GP73-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="9"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8132834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="332" mass="39460" checksum="97BE3A2D2C9E9D06" modified="2022-10-12" version="1">MYIYILLXEEQLRRILEEKDKKEKEIQMKKHNFQEFNQKQITKLKEIISQGQNKQQQSEKEKQDQLKKQKYLLEQARSKILTQNQSRIQEQKNLEKEIENKFKSTSYQKIIEKYQFGLNSLFQFLIKETFLPIGQPSSREQITFKTFAWFTDRFNLCPEIVSKDDVLQLFRYLTRSKEVIDGIPPGMNYDEFLQALHRISIKGSVIFNKVAVNVKSQKGKLDIKTIKQIVEAEQIVDQEPQSQSPKAESIVIETPLRNKSKSNYLESSKILKEQQIFFQQSNEQTFESLILYLDVSNDKNQLDQRFRKTLEERKVPTKLRRKILTEKLGIQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S2K7</accession>
    <name>A0A8S1S2K7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0030302</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S2K7"/>
    <dbReference type="OrthoDB" id="305201at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="66"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8134428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="313" mass="36992" checksum="DB0630114A31A127" modified="2022-10-12" version="1">MYKGYPTEVKSSAGQRITRPYSQQKEYIDMKSSLKQQSQSCEKSNQSTILKGKVSNKQQVKNKMKPTQTRFQFQQPQSLQTTHRYNDSPYVKPHQQQQLFELQKTIMADSNRVGNIVNQMKFHSQTKKNPQQPQRQHKSNQTNCQEKQSQSLNGSFELKKRIFTTNQDVTSKLNMITKSNRAQSVMDEYKTTAIELQQTMKIPEPILPQRIIPYDSETEFEGYRKGDFLTFESEDESENEDFLRKAGIEHFLKNLTDKEILLLEKYIYLRKYDMMARNPKTSKRNEQFLNNNTSRTSQQYQQQFCKISRQTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S431</accession>
    <name>A0A8S1S431_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0030103</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S431"/>
    <dbReference type="OrthoDB" id="301729at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8134040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="365" mass="44404" checksum="C1BF9349B963FF9B" modified="2022-10-12" version="1">MLQFRGFMIFDLKRSIFFFEMRRDIELDFKWFTLQKEIRKKFIDQDVLEDKVLEMYSGYGKVRGKFDDFQKRFLILIAQNSVEDIYLAELLNEIFAILIVQEHYNKFFKEELEFQGFFEVEKKIQEKEYYLNQNSYSKHVIPSLFQQIQQYKELSSSNDLDQLLDEQQQSDSFSQKQIIYKGFTIFDTFRQCFYMLIKRGFANDNLWKMQRMQIQQFLLQRSKNPLQHFFLNSEMGQFLFEYDIQAKNYFILITNSSSAQHPQCQLLQKLKALVQRIPNYQKLYKPELENNLKQQLLGVIEAEERIYYQIYCPNWIEKEKKINRKIIKQRGLTEMSFKNDGIMCFDEFSLTDYLSQRDKFVPDFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S6G7</accession>
    <name>A0A8S1S6G7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0040047</name>
        <name evidence="3" type="ORF">PPENT_87.1.T0040049</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S6G7"/>
    <dbReference type="OrthoDB" id="439265at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="HEAT" evidence="1">
        <location>
            <begin position="32"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8135087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8135091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="58" mass="6294" checksum="7436AA2C30F0926D" modified="2022-10-12" version="1">MSKDSVANIRSNVCKTSILLAKEKGGNVVEPLKKVLQSLCDDQDAEVKYQAKSALESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S716</accession>
    <name>A0A8S1S716_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN motif protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0040262</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S716"/>
    <dbReference type="OrthoDB" id="287158at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF263">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="447"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8135515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="467" mass="54474" checksum="1FB870D1CF94F06B" modified="2022-10-12" version="1">MQQQSISDFDNLEQEHQLIDQVNQKSKSAKFKQYKQGQYYGDIQNNVRHGYGLMTYQVRYYIGFWKNDKKQGFGKEVLDKGDSYEGQFDEGKPHGEGTLETENGIYSGQWVQGIKQGYGKWRGKNNEIYAGEWKFNKANGYGRYDYQDGGWYEGEFKNYLKYGKGKESYANGDFYDGDFINDKPDGFGVYRWSDGSSYHGTFFSGVRHGKGYWLKNEDSQNYESYDGQYVNDLKSGYGVYRWSSGNVYEGEFFNDHRHGLGEMRWADGSFYKGQFHNGQMCGEGELKRNNKPIIYGYFDNNKLVKEQNQKSIPITINQSQNNSFDGKRNYYSLQQSFNQNQNGQRHQKSHFKSECIEPISKQTKRNCNTSLDDNQIQQHQNKFKISIDFDDHQGFSTQQKFKTISTTNRPFQTQQEGLLQVTNRAQSTKKVIIESVKFKRSQNSFRLQSLNQSTRQTQNSLPKQSIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S7Z0</accession>
    <name>A0A8S1S7Z0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GrpE protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0050018</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GrpE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S7Z0"/>
    <dbReference type="OrthoDB" id="201635at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00446">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.22.10:FF:000002">
        <property type="entry name" value="GrpE protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01151">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000740">
        <property type="entry name" value="GrpE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237">
        <property type="entry name" value="GRPE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237:SF23">
        <property type="entry name" value="GRPE PROTEIN HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01025">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="35"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="36"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8136178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="273" mass="31698" checksum="6D3A3B7BA17CD24F" modified="2022-10-12" version="1">MKGLRIGARVFSLISSPQQVFFRQGLHQTPIYLFSEKTKKQDTPKNDQKQEKAQQKEQKVEQEKKEASTKQEEKVESLDESHEDKLHRVESFYSKQIEKLETQIKDHKEKIHDQIKLINQLEASNKDHNSKIKELRDALKAELEEQELQQKRISKEKEQLKVFAISNFAKELLEVQDNLERAIASTTDKPEENPLLEGVVMTHSILEKVYKKFGVQKMNVTGQKFDPNFHESLFQVEDPEKEPGTICYVAQEGYVIGERVLRPAKVGVVKQQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S904</accession>
    <name>A0A8S1S904_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0050210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S904"/>
    <dbReference type="OrthoDB" id="10249433at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF197">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_38, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="201"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8136558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="424" mass="49385" checksum="D7A17085172666AE" modified="2022-10-12" version="1">MDLDFIFFPAPKETLDNGYFDEGRILWIPKHSALYPNFQNYVKRKTKKNMVIFLRQSTQQIEEEQLALIDDQNEFKKQPIPQFPNIHHSNKQNLVKNIQVEEKEIGSLEFDLIDQEEDLQDKVTKLNQESVYNIGKSKINNIKQNSFMRNKKRETTSKSSPHFLQFNQKQEPTIQQIKTGQRDGKIIGYIPCLLIKYENSSNIIVYFHGNAEDISQSYAFLIHLRNQEKISVLAVEYPGYGKYNQVQTSAEAIQNDADYIYNYLSKKIGYEENSIMIFGRSIGSGPATYLASKHKPGCLVLMSPFTSLKDAVRDYVRFVGTWVQHLIRQRFKNLDNINDVTSPTFILHGKKDDLIPYQQAQKLQENCSAKICVLHLAEDMDHISYKLHSDLIIPLMQFLRKINFFQIFFKSPKVPTKLYHNPIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SFF0</accession>
    <name>A0A8S1SFF0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nop domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0070179</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP5/NOP56 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SFF0"/>
    <dbReference type="OrthoDB" id="6780543at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.90:FF:000005">
        <property type="entry name" value="Nucleolar protein 5, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.4070:FF:000001">
        <property type="entry name" value="Probable Nucleolar protein 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045056">
        <property type="entry name" value="Nop56/Nop58"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012974">
        <property type="entry name" value="NOP58/56_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012976">
        <property type="entry name" value="NOSIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894">
        <property type="entry name" value="NUCLEOLAR PROTEIN 5 NUCLEOLAR PROTEIN NOP5 NOP58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894:SF1">
        <property type="entry name" value="NUCLEOLAR PROTEIN 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08156">
        <property type="entry name" value="NOP5NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00931">
        <property type="entry name" value="NOSIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Nop" evidence="6">
        <location>
            <begin position="284"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="410"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="444"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8138566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="468" mass="52378" checksum="600FA69E3FEEE2E8" modified="2022-10-12" version="1">MLILIETPAGFALFQVANTKALNKIDNIYDYLQNEKQAKKLITPFAFQQFKDTQEALIATSKLINGKIPKKLSKFLEKNVISQEVQDQIAVQDKKLAKQLQEQLGLSCIQTPVTDQLFRGIKSQLTNLIEGLSEAELKSMTLGLAHGLSRYKLKFSTEKVDTMIIQAIALLDDLDKEINNYMMRLREWFGWHFPELGKIITDNLIYAKVVKAIGMRIKTSQTDLSGILPDNLEADVKQAAEVSFGTEITEEDEKFILCLADQVIELTDYRAQLSEYLKNRMQAIAPNLTTMVGELVGARLISHAGSLVNLAKYPASTVQILGAEKALFKAIRTKHNTPKYGLIFQASLVGSAPAKLKGKVSRTLAAKTALCIRYDALGEGQDAEFGITNKAFLEKRVHQLEEGVNYRDVKAPQRGKAKPVSSQAQYQEEADFQPQGATWMQRFSKGEDKREASQDLAQNSKFKKVKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SH48</accession>
    <name>A0A8S1SH48_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0080318</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SH48"/>
    <dbReference type="OrthoDB" id="309119at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="25"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="183"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="86"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8139835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="236" mass="27896" checksum="42E3E5A015E75465" modified="2022-10-12" version="1">MLATFNLAYIQREINSTIQLKLSKLMFAGLIFFFTVVFILNYIDYFIQQDNYHLCQGYFKQLLIAQFFNVITTVSFLLQGLFLLQIKQFTRQINKNIKNLQNLNDETEKIIKVIKKRLSQIWILVIINIIGSFVSFVQNIYFVLMNEILRNQSERYTCYYVKIEDDPTLSNIINTTLELSEKMLSFFLPYFAALIIFWPNKIKNQISDSRQDSVEITVETYVIHTPELLDQSSSRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJM0</accession>
    <name>A0A8S1SJM0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0080109</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJM0"/>
    <dbReference type="OrthoDB" id="5979581at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001088">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="15"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8139459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="422" mass="49086" checksum="639FB29C7E63AD1D" modified="2022-10-12" version="1">MNSPSTPNKIINNQFVLKKKLSAGSYGVVFEAEDLLKKQMVAVKIEKKEKNSTLDREIHILTRLLGTQGVPKLIWSGIDNNSNVLVQQLLTKDIGAYLKEYRTFSLKTVLIITDYLLQIIKRIHNKSVVHRDLKPENIMYHQKQIYIVDFGISKLYRDNNLKHVPFKEGRSFVGTTRYASIAAHKGQELGRKDDLESIIYIDILLLKGTLPWQNMLGQNNKERQKQVGEKKMKMTPSEICVDLPIEFSKALEYIKSLHYQSDPDYDYLIQLFRKLGQSRRIEYDDVYDWTQSQNQIPANNSKQETTIKQISSLQQFERSPRVMQNKCQSLKVPSTSDILQDRKRSLQNTSQLVLLQDELNKSIQNSYELSQIANDYDSKQDYVSQDDANDTLFQRYNQLKSQILQIHHLQNIKNQNNNIMVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SL11</accession>
    <name>A0A8S1SL11_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0100055</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SL11"/>
    <dbReference type="OrthoDB" id="297431at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8141325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="240" mass="28408" checksum="9A6F22A63BF2757A" modified="2022-10-12" version="1">MSIKHQQIDEPLYILKGFKIPLLNTYFQEPYLSPINVKQQLSSRCNNQPFFESSSTKANATSREFFRFESQKVDKTQQNKQLYEKSLSPNKPKFLEDNKLKGLLNIVSDRNKQIHLKKYDFPIGGSTTNTKKIKFNFKKINIKIPQKIQFSKEDLENNKKFLINTLRKNSVIQNQVDIERPVRSKSDFEKARSKSNLKQVSPFKDLFIEEQQKKKTVRFNELVEIKMFDRIKSHLMKQLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SL42</accession>
    <name>A0A8S1SL42_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0090206</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SL42"/>
    <dbReference type="OrthoDB" id="305682at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="196"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="324"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8140638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="518" mass="62621" checksum="9B7EF72BC5C1C875" modified="2022-10-12" version="1">MQQNSSFDDKVRMEIENQFELRDESQELAEDQRLNKYLTPQKQDNFKLDDKIDDGQESPHFTCQVKNYRESTEQKHNSEKENVTIQKKDEFQINMHFTPKKNECMFQELNNLMIQVQERIQNLSQLSPCTNEISQCNGTYSEIKQQLFEIEKVYRSKDDELSQLNIQLEKEFNACKNQKYLDQTTNSQMNQSFDIIKRYQQLKEIKKNKLQKFKKNYHEQYNQLRSELQELKCTVQQMFLKNSFSLEKEQQIMEFCKVFNQKYRFIEEISFNDYNIPVLEDQKQESPKLKDEQNLNSFIQILMKQFNINQGDIETIKKQIIECVINQKEMIEQQQKQLEIIQTNQNLSANQQINKLKTINDELQNELIQLNQHKEFQQKTLDQTKKEVYELKQQLEFIQKIDYVILNTKLLQFEQTQMKQQQRQIQITSIIFEQLMQSTSFKSQIKDVLNEQTQYLKIYFNKAIEIINKQNLQQDEDPLKYLNYHQQTTQKMLDTICIEYSKMIKQIQQQREEFMSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SLZ9</accession>
    <name>A0A8S1SLZ9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Tyrosyl-DNA phosphodiesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0090108</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tyrosyl-DNA phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SLZ9"/>
    <dbReference type="OrthoDB" id="47785at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017005">
        <property type="term" value="F:3'-tyrosyl-DNA phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09122">
        <property type="entry name" value="PLDc_Tdp1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09123">
        <property type="entry name" value="PLDc_Tdp1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000081">
        <property type="entry name" value="Tyrosyl-DNA phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010347">
        <property type="entry name" value="Tdp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12415">
        <property type="entry name" value="TYROSYL-DNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12415:SF0">
        <property type="entry name" value="TYROSYL-DNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06087">
        <property type="entry name" value="Tyr-DNA_phospho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="28"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="427"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="429"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="11">
        <location>
            <position position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR610347-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR610347-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR610347-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8140506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="522" mass="61088" checksum="F220E5E9308367F3" modified="2022-10-12" version="1">MKKRTLQELYEEQEQINSSYMDVNSLQKQLYLENQKRLQKENEEKEEQLKNDGDEVKQVDEKINLCFPVGKFCLNHLSQYPKEEKQPHILSFINIIQENAFQDIQKEKPKITLEAALMTCYGFEDHLLKPLVLSGVKLFIINDNDNNDKKLEIIEEFNGHQNWTVIKPSKLSSITFGGSFHPKIWILKFPKFIRIVIGSQNLHVGDWTVWSQAMWIQDFQIGNSELDEISKDFKVGLKEFLDNILPNSHKFEDLLKIKYNDYDFQNINIRLITSIPGRFTGNQMNKYGMMRIQSVINSELKSNNIDIPKQVSITYQTTSIGQLDINYIDFVQQCCSGQQIKVTQKLQQNNKSIAQMLFNQQEEEKSILKLIYPTSDYIQNQTSAGPEYANPLFLRKQQYENPKFPKNIFYRYQGSNYYYWHAGNIPHLKVMIITGLDEKIDDKASIYIGSHNLSQAAWGRLEKNATQLFISNTELGVLYPPKKDSAKLKQSIIEQMSFKFPPDKYEKTDQPWISEVYYEQQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SMN7</accession>
    <name>A0A8S1SMN7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Fused homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPENT_87.1.T0090448</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8141118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SMN7"/>
    <dbReference type="OrthoDB" id="266718at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14002">
        <property type="entry name" value="STKc_STK36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000292">
        <property type="entry name" value="Serine/threonine-protein kinase 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001384">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22983">
        <property type="entry name" value="PROTEIN KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22983:SF6">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="4"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="13">
        <location>
            <begin position="1019"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00075375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8141118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1133" mass="129902" checksum="AE5F15C760A9885F" modified="2022-10-12" version="1">MENYHVLHLIGEGQFGKVFKGRQKKSNQMAALKFISKRGKTEKDLANLRQEIEILRRLKHENIILLLDAFETQGEFCLVTEFAQGELYEILEDDHNLPESEVRKIAQQLVRALHYLHSNRIIHRDMKPQNILLSANGIVKLCDFGFARAMSTNTQVLHSIKGTPLYMAPELVQEQPYNHTVDLWSLGVILYELFVGQPPFYTNQIYSLIQLIIKDPVKYPDNMSPEFKSFLKGLLNKIPSDRLGWPDLLNHPFIQESDQEKYERKKRLEQYSQWAGVESEVIDKRKRSLTPTREIITYDQGKDTRDVSPRRMPCNDEYWVKCEQIANDIQGATQLRCDPGFLDKLLQVLAPPKKPSLHCALKVFGQIITKGNLQEGLDVVKNQQIPIQLVTHVKSILKQGSSELLSELIKTIGLLAKATFDKNIGIDNIFIKFIGMTAQILKIGQNNELINTIKTIGIFANQASLNPVRSVLFYKELVEHNLAQETAKLTKSNVILHKFATQVIGVLIHPIHGEIFSFPWKKGYSIQLLNFNEALPLFESLKNQIVASYADIDHLKIYNEQDEQQNLTRISVLRILLQMIRVSKDVHVPKELISQALNSDEALFQGTALLILVQQYKNKNQEYQVNLQQVIEIFENNIQINPIVSLSAIQLIAELLQQETNFTNQLLNYFSQQLPYKLLNDLVNPNKKQNRTEEIRKLEGSSFGCPSYGFVDGVITLLQKLLFRYNKEQKKLTDLFQLLEKYEIHQLLFQILLNITSRNDVSPKGFVSLLILIHDSIYSNFKALGQLIFQDNSIKILCNFLKEAQLQSIQEWPSSSGGGMACVNVMSIQIIRIFILAFQEQNCDNVFKELSNQEIVALTLNLIKYLQKDHISLVISFISRLVGNNEEDKQFAQSFVQNNGLAILQKYQIMSVDSINILSQLARISKDFYPNIHSINLYNDLKQQIQSPDSNIRAKVCNLIGNLCRHSSFFYEHLLKFDLINLCIKCCQDPDKHTRKFACFAVGNAGFHSDRLYEQLRPVVPMLVELLRDQEEKTRANAAGALGNFVRNSNALTKDLIKHGALNQLLELVKTDKGSQPTRISLFSIGNLCAYPECRKKFEELQIRQIIEINLASTKDQQIQKYGKRILIKLDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SNG6</accession>
    <name>A0A8S1SNG6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0090381</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SNG6"/>
    <dbReference type="OrthoDB" id="297971at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010960">
        <property type="term" value="P:magnesium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045095">
        <property type="entry name" value="ACDP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064">
        <property type="entry name" value="METAL TRANSPORTER CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064:SF97">
        <property type="entry name" value="METAL TRANSPORTER CNNM-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8140986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="153" mass="17891" checksum="436454EC49E196EC" modified="2022-10-12" version="1">MNQSSISFIYLNAFTNIIGTIRSKQLIDMELTKRKISELDNLVRPVLFIQNDTSLFEMLMIFKQKKTKIAFVVEANKIEQTNTSRIPNYQIVDEKISKKIIGLISLKMLFEEIVKKEFHDQDNHIKFNSLKPHQIQPIGKSQNKILIEPEEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SPM4</accession>
    <name>A0A8S1SPM4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0090067</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SPM4"/>
    <dbReference type="OrthoDB" id="10390843at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="43"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="486"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="69"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="203"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="56"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="486"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8140424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="579" mass="70571" checksum="A3A61069853CFE29" modified="2022-10-12" version="1">MENRLEKNVNKLIERYKKTSQQLDQMIQPEQIISQPITFYQTRSRQSRTNFNDEESSSREKKNHSIEQMSRLRETLNAKELQEKEKQERLRILNKELKMTLKDYMQVNKDLEVKINQREKHIKQLEFELKSIQDSFTKVENNVRENRQKCEGQEKIIEELQQQYHVSHQKLLLKKDKILLLKEQLKQQEIQFDEQGQNFGKELERVQSLCEDFSSEIKNQEAQNRELEEEKMQLLEEKQQKEAQIRYFDQLIQDFRFRDEDNQKQINYLNKELQYQKAQTQQYIIKFEECQEKLQQVSESTQAKLLNLEQESKKYQDQFQEIINKKKQKANQQKLKIDQFQQIIQELQRKIDNDEKNIQIQEYDFQKLQQISNEQKYMNDQQAQKLLNNEEKIQQSLLEIQDQKQINSNLLSQIKQMEQRSYQDQRIIEQLKDDNQNLSDSLIQVQNYNRNLEQEHLDRKLQHEKQMDEVEKKIDNLVESVQNAKEELKEQKQRENDLKQKLKQSELETEKHQKKSQKYKQQLQNLIHTIKNLEDKFKQYDCQTQQIQFQSQKQGELHNQQKIKVLEDIQSLIKLHRKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SQN2</accession>
    <name>A0A8S1SQN2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0100265</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SQN2"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF30">
        <property type="entry name" value="PHOSPHORYLASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="79"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="464"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8141706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="529" mass="62049" checksum="863434B7A3848762" modified="2022-10-12" version="1">MDKKGNFERLRQKLKSDLFKNESNQKQYAEEIINQCPQILDYVHCSSQSEFKEEPTQLIDYISNQKHLEQMKHSFQNQYQIGKILGEGAHAVVRQCWQITNPDETYAVKITRNPDPEITEIMKQTFLNTVSLNHPYICKTNMLYIDPNMECSYLVMEYLPYPSLQQILKERQKLEFEEVRLIMRQLFEALSYIHKVGLCHRDIKPDNIVFDNDSNTIKLIDFGVSKRFLVTEKSCKDIKNNLLWTVTGTMPYQAPELWTGIGYSNKIDIWAVGVVCYQMLCGRLPLDQENQMESFSTQTEYTGHFQEESFIKLPPLIIDFIKRILKWDPDKRITSQEALLHPWLYQPKLMPKYYKGKSKDDIFSRSSDKPLLALVRSMQANVKIFQQSVSSVNEHFLDKNNFNKNHGQIIIGFKETSQQIRDKSIHFQQSIHSMNLNKCTLKPNTEDVKDFFDIISYCDSNSSFEALEEKQQLQVQQQKQAQQQQQQKQAQQQQQQKIAFSQRLGIIKEEPETLLVNSFIPNIHSEVQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SSB2</accession>
    <name>A0A8S1SSB2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CHCH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0120045</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the COX19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SSB2"/>
    <dbReference type="OrthoDB" id="268594at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010625">
        <property type="entry name" value="CHCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051383">
        <property type="entry name" value="COX19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21107">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21107:SF2">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06747">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CHCH" evidence="6">
        <location>
            <begin position="26"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8143113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="116" mass="13715" checksum="6B5981C0E9733C34" modified="2022-10-12" version="1">MSNRSKQNASKGPDKGAFPLDHFHECDNEAKQYNQCIQKHENMPKRCRKYQVDYLQCRMNNGLMEKEDLSKLGLGPETSWESEEQEKQFLFDKINRIKTKAMDEVQKKTEQSNKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SU67</accession>
    <name>A0A8S1SU67_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">T-complex protein 1 subunit zeta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0120255</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SU67"/>
    <dbReference type="OrthoDB" id="296019at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03342">
        <property type="entry name" value="TCP1_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000038">
        <property type="entry name" value="Chaperonin containing TCP1 subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000058">
        <property type="entry name" value="T-complex protein 1 subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.260.10:FF:000017">
        <property type="entry name" value="T-complex protein 1 subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000004">
        <property type="entry name" value="T-complex protein 1 subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012722">
        <property type="entry name" value="Chap_CCT_zeta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02347">
        <property type="entry name" value="chap_CCT_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8143490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="532" mass="58476" checksum="1745C5CD8BEDF766" modified="2022-10-12" version="1">MSSVQFVNSKAEVLRKYQALAMNINAAAGLMEVMKSNLGPKGTLKMLVGGAGQIKLTKDGAVLLSEMQIQHPTAAMIARSATAQDDIIGDGTTSNVLLIGALMKQAERLLAEGIHPRVITEGFELARKEALSFLDTFKYQQIDKAVLINVARTSLISKLTPDVANQIIEIVVDAVQIVHVPEKPIDLFMVEIMHMQHKMGAETELIRGLVLDHGARHPDMPKFVKKCYILNLNVSLEYEKTEVHSGFFYNSAEDREKLARSERKLTDDKCQQIIDFKRKVCEKNGYGFAVINQKGIDPVCLEMFAKEGIVGIRRAKKRNMERIAKACGGNSVNAVEDLTESDLGYCEVLREYTLGEEKYTFIEGVQNPASCTILIRGPNEHTIAQIKDAIRDGLRAVKNAIEDKCVIPGAGAFEIATSVHLQKFKDSVAGKAKLGVQAFAESLLVIPKALAENCGYDVQETLIQVTDEYIKNNIPVGVSINEQGFIAPIANGIFDNYCCKRSWLNIAPTLAQQLLLVDEIMRAGKQAGGAQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SY85</accession>
    <name>A0A8S1SY85_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0120347</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SY85"/>
    <dbReference type="OrthoDB" id="286296at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049942">
        <property type="entry name" value="DML1/Misato"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13391">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION REGULATOR MISATO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13391:SF0">
        <property type="entry name" value="PROTEIN MISATO HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8143674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="423" mass="49675" checksum="30AF6FE5C5B4057B" modified="2022-10-12" version="1">MILSLFLGNRAVNLGITSQYLDLQNKFQEEDPILHQFDHNKRPNSLYFGNDLDLSKSLNMINMNSLKQETQEVPIINEQEKDEPQIIKLTQKYLHEMRIDQNQIYNYKKNFENENDLFAINFDTTQVELFQDNIQTQLEKADQCDGFELIVDCETCYGEIGLKVVDSLNDLQGNSKSPKLVISIVDPCNNIKNRIINRSLTLTQYQQNSTLLVPLELKDMQIHLNQIELNQQYSFLINNIICSYKCKQEYVSMHEFCNRITSAPQNNIFNILASIPSIQDKPDDSILNYKQIKNLDKFYISQDIINLNSEKESFGESYFLRGISPIPNERNLKSYGVDIRILDYIYQVPMLYNYIDVLPFHSCIFSNESYKFTLLKLYNELNSIKKMGFWQYEKQSLEDEQQLKYDLFQLIENYGGVDEEEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SYC4</accession>
    <name>A0A8S1SYC4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0120241</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SYC4"/>
    <dbReference type="OrthoDB" id="306791at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8143462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="294" mass="34240" checksum="D9C24EDD431543DF" modified="2022-10-12" version="1">MDFKDQSVGICYCCIFLTFKHYFYLMMITNAIQGLILMIIGFLGVLNLIELFNFSLPITITLLSLGSFLILGLIAEIFFFFRHSVIKIGRFMVVLNILTSLFDLILGVILLLICFFGVVLIELNDTQLYFSQKRRDILKVISIVIIFISFFGFWMTYLQYQLLLPLHEQIQQDIKIVPVVSLRQAVTRKFSKNFIAELTPNNKQQKRIARADTISEQSQPLQSIQLKSQKNDEMPELYLNQKSEKLIKIPDSVSPYNEYFAPSTNTIRYSNQIEDIQNLRKQRTPRSTYLKQQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SZL1</accession>
    <name>A0A8S1SZL1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0150333</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SZL1"/>
    <dbReference type="OrthoDB" id="296591at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8146415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="212" mass="25407" checksum="BFDB855B57C722B7" modified="2022-10-12" version="1">MGQMCSTGKKIKIKIYNSQEIMTILDKEQTRKNLDTISIQFRNSIFNLFQMIDTQMSLNKQKLLFETDRSNAHNSIHKFLRILINQYYIPFQQNFMNVTYPIIELCVVKILIESYLAMKTLNSKEEIWWNSKYFLFRKSVLIQEFKMDVKQQKPSIEAPFIEYLQLLIDIIEQQLHQGCHNNQQEKKNVHQITESHTETNIFYSELKRNIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T355</accession>
    <name>A0A8S1T355_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0160108</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8146900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T355"/>
    <dbReference type="OrthoDB" id="118550at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8146900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="16348" checksum="4F3E1CB3D4B1BC07" modified="2022-10-12" version="1">MKSENSGTLNQEYLELTINHPKQQDISNSNNVKQGHWTQEEHHQYVQFLLRVKGSGDSQRKGQPLFKRMSQIIGTRTPSQCRSHHQKFNPFNPRLRRNHKRRQLAKIEKPFQRTKQLIRQYFNKHKIHSDDENHNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TCS0</accession>
    <name>A0A8S1TCS0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0190231</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TCS0"/>
    <dbReference type="OrthoDB" id="295853at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="183"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="272"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8149678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="744" mass="87077" checksum="196027F49ED1BD48" modified="2022-10-12" version="1">MQNCIQPQISEIQTNHYFDLISVPPSMRKQSARVDQRPIQTSKMYFESDSNIDNTQSLHSLLQQKEAQLDSLQRKYLQIRQVKSRKPQGQHFMHGYLDDLINSFKDKVDNNIISEFINYSNSMNDIYRMQQTHLHLCQQRLQQADSLVDQLKILKEADLFNLKKIVQSLQESLQQAQVTELDSNKLYRDLQNMKLKYAKLQNQFEIVQKQNEYTEKNMQSIKQHLQHTNEETQVYKKTLRKEASQNQFNQRRVFTLEQRLEVLSGSDYKKDKDSLRNIIEDMLKENEANKRKAFQKSQEVSKLEQTVQDFKEKNQKLQKRNLLLEKQLKLLGYSAYDLNGNTMGQKTIVESNDLDDESLDIFQQVNLPNKLDFKLNNLKLRQRNLVQDLLDCGIKQAIDQLFQMDQQHQVDQISIFMDLFVQYKNLGESVNSIINLIKQLLKIDQIEVIMQKLSSDSQLFNCELIQLWLIDQQTATFYTYNQKNELVNAYIDTDEFLNVVKSSQPIYKQQAFVYRNINTAKFAKDCYLIPLLTDSRLIGIISLENLNNKKQDAKYLGLLISQLLLAIIDKVLQFKLINYQLRYKNLLFEAFTELSKQKSKYKLQKSIENHAETLFGVANSRFFFYSQGIIYTYKDDMHTTEYSSDIGVIGYVAKTQEALLIGNIKQHHSFHQSVDMTSGLPIFTLPLAFGVLQIVLNQSAQLQQNNKSVEVRKLLQPGETLQSMALMFTQICNFAYQLLQKELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TDS0</accession>
    <name>A0A8S1TDS0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UBX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0180370</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8149022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TDS0"/>
    <dbReference type="OrthoDB" id="306351at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01767">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050730">
        <property type="entry name" value="UBX_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322">
        <property type="entry name" value="FAS-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322:SF93">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="303"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="326"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="303"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="313"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8149022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="469" mass="55477" checksum="ECA0667E2311A547" modified="2022-10-12" version="1">MQATQEKDILDFMALTGCQDEQKALQYIQMGDNNLENAIQLYLDLEGQPTLSQQSSVQSQKSPQQQNQQQYLNQQDVKEVNTMIFNQNNPPPELIRQRSAPDTTLIEKYKKYQEDKRANDDGILKKTFKFGWSAITYIFKRETNHGMAFQTFIQQQQIQTDINFQMGNFQDNLKRAHEETKPLLIYLHNQQSLYTFENMINSKTFVQIVNKNYHIVGFLESPQVYDLLPVKPQPPTLLIYRLDLTEQPILMDTIHLSPESNFQELAGKLKSLKGQFNKQFIMEDQVKREFNIPQQYIPNFYGQQQQQQYSQRRQQDVQQREREQLIKQQQEAYRIAEQQAAEKKRREQEQKQQQENKLLEQQQLSEQRLLQKATILSNLPDEPENEEGIEILFRFENSNKTRRFNFNDKIQSIFDFALSQEDDCFNDPNSNIDLIQSFPRLSLKDKKDEIISDVFTDSNKVQLIVEECE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TFQ1</accession>
    <name>A0A8S1TFQ1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0200058</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TFQ1"/>
    <dbReference type="OrthoDB" id="307541at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8150036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="180" mass="20637" checksum="49935EEFAECC8B45" modified="2022-10-12" version="1">MLRDISRNKGLYKGLFLTFGLVLIIFCFFLNVSEADKTLTKVKYFYTFSARVHTSLDGKDCYEDILNCQDDSFCKTVKTTPYLGGFTLGFMVLVLLFSFGESIISRIIKKYHIWIIQLILLFLAWALILIIPILYLTTKGKNWNLCWLPIIFEFFAVIATSCSAILYYKSNESKGPGLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TH17</accession>
    <name>A0A8S1TH17_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0240207</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TH17"/>
    <dbReference type="OrthoDB" id="301636at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053080">
        <property type="entry name" value="PP1_regulatory_subunit_27"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46899">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46899:SF3">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="770"/>
            <end position="802"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8153421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="804" mass="94176" checksum="E0523E21EC87B84D" modified="2022-10-12" version="1">MIKRNLKWRINSQRENSTGKPKSISIESSPRQPIYLIRREVKPKNSQLKSPLSTYTKYVFRSNQISPVSSPNTSKIIKLEEQQINFNNNNCNQERKHSISTLSTENRSFKHKISANNRHLQTESIKLITQQFTEQNVDIIIDNEIKQQQIDIECSNIDMQHKKISSNYLFNDKKKKQLRQRMQQFQSKEQRRSSQLTDQVMKLIRTIKQPSYRYEANQRSSRIWLENACFEPVKLQENYTYSQSQFKFYFSQYFDKHLKFYRILEINNELLQLMNNHQQITKDIQDQRKAVFLDKIYSNTQIQLYKTETLFRFQNEIQTYGMIDFLNENSQLSISVDHNTSELNDKNNKEQLQIDEDEDSELFKQAKTHSLSNLIPIKPEQNTSLNNYQFQLTSIISELDKNSKNILLSVDYTNIDQQIYKDNINQIVPTFQEEFSNPIFYKVYTQHYNKNYKDILNGNFCNLNPIDDLEYEAPDLYVIEKGTLQKEDGIDKKKMLNSLRQKKNWHSGVLQKQTQPIDTISSHRATIQVFSNKQSSDIGVDSPQLPQINILQQSSNEQTINKSIRKSIHCRNALSPTSPGNRDNKFQQVDNQIKNQIDTSQDSVGEIKKITMSKSQQQLPKLMFESNLTQSKILKKEDSNKNFKDFKMVSSIKSDFVYNDSMSDSVNSNMQQINLQNKGANPQKIKLETMLLYDKMMKRNRSKKKIAIIMIEHGLLNQFQEFMDVHRNFNLNGITQDGIPLISLAAANGHEGIIRHMLNFNVNLNQIDKNGNTPLHYAMLHNNYEIADLLLQNGANPYIKNQGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TJ79</accession>
    <name>A0A8S1TJ79_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0210267</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TJ79"/>
    <dbReference type="OrthoDB" id="294199at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 157-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 116"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="213"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8151266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="739" mass="87209" checksum="45AD86E91B36BAD9" modified="2022-10-12" version="1">MQLSEKLIARVFVIILCLLSITGSTLILLKFFRTPRLSKNPGSIIQRMTIAQIIITFLMLFAQFYIESNDKITGGTFVISSNFCSFLGFIEIFFSSEYILYNVYFPINLYCSLKTQDYNFTKYFKFMEIFSLIFSFLFTLTMFFVKDVKINFVGVCGLLLNELSIPLSVILAFITLTILILLVFVITEKSSFKTTLYNKDSEKFHRKYHKEFIIVNTLYTTVFLVTYMIPSCLIFWTQLIPGDENRFYSFPIIYPLGGILLFLIRINDPIVKKYIYTIIMGMKKKNQNFKLKDKLLQKEDFNGIEQISINSGLEISVCQNKKLKYTVKPMFSEKQLQSEIILSSTLGNGIQNKLIKNNQELFIVLLSIKNVINQCIQEKQSLLKTLKPFNFIHITKYQLYLQDDYKELDQKYKNYNNLCLKDYVNQICYKRVINCYSYASQTLIHLFSQILNIDLNQLKISLKIEQNTKKLINTKLSQFYGPIFITYDNYFSIEIISKQHKLLLTKGGGLMNICKRYQTEYSKQKNGNTLLPAILGLYTIQIEEDRYINVVLKLNQIKIYYPLQLNFFNIPEEQDQLIQQDVFGWIQLSLDKGQLKLFIAEKLFDDKFQIKIEDNDFKLSKNATKDLLTILSKDIEEFCFCRNLTLSLVYFKLPTNRLDSMCKHDNSLSYHYQIIKNSQQMTPLQMNEYFKDLGQFELDSKIGFVRIYFDNFWQDWEYIYQNERCMYFDKLTEQLSEII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TMD9</accession>
    <name>A0A8S1TMD9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0240206</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TMD9"/>
    <dbReference type="OrthoDB" id="285788at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8153420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="210" mass="25236" checksum="8C57EF83E183BE2E" modified="2022-10-12" version="1">MNFHSNWRYEKMQLIKDVLVNKNVVPIYIQNRLMKELDFQIKAFANKNKNSLSPKKSCNRRIKTLDQSPQRIKFERSVSRSYNKDQDCLRCRKFNFQNKIIIQCDTGQHMFHEKCLLDLFKEQLQDIQPHFSCSCGMKLCPEFVKELNIRGIEQLVNQLYEQQLKCITYQVQIQNCLNPLCNFFWITNSQKSRKYGRQLKPLTKKYCPYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TMQ6</accession>
    <name>A0A8S1TMQ6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0220384</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8152309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TMQ6"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8152309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="172" mass="21070" checksum="FEE188792CDE71B6" modified="2022-10-12" version="1">MTLLQQKEIYVRKLSGMLRLLSGQNQILHKYLNAIDFLRKIKLNTIKLQENLQFNTKNLFRVSMTFIFSSIFWNLFTQPFFNILQFQRLRSLDQMQYQKQQLIHGEQEIRKTYFWIKQRIQRIKILIQDMLQYEEWDGKHITNLFNDILEPIKNRFLKKLCVCKLYSNSLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TMT6</accession>
    <name>A0A8S1TMT6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0240126</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TMT6"/>
    <dbReference type="OrthoDB" id="309085at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8153283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="295" mass="35315" checksum="B51FEE37CA5D9D7A" modified="2022-10-12" version="1">MKNGILLKNKLSFSGIKKLLLQLDELQELYLATMEKTQIQLQNINYYSDTKLNITQIYSSNKPFELIETLQQCLIEMKNDKSPKYIKLNKKSKQLIISLNKAKSVQQQINQYLEMLSNLVNKLNYKKECQILITSISLILKKRQKFQLKLLQKNNQLSENLNSLSQLVIKQDNTKDNVKQYLEIKTLQNILDQKVVVKTQEKKQKIEQLSQSVKIKEEFENRGVYQHNSINQQLYQSQQQMYYTNFIYQMYQQYCYFNQLKQTECNILNSQLIYNQQPFMYNYCPQFQQNFQQQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TQR4</accession>
    <name>A0A8S1TQR4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0250327</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8154348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TQR4"/>
    <dbReference type="OrthoDB" id="312324at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF18">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DST1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="164"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8154348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="440" mass="51959" checksum="AA28965ADA4B04F9" modified="2022-10-12" version="1">MGNKQSQESLSSSKSDIKLFDVKKCISIQRDPVTRKLIGVPKEWASHLDADQKYIVETNKLPEEVRTHELPDCILEIQNSIYNQKNLQKELKIDTNADLCLTGLNVEIEKEIYNSGVSRNEIENDTMNFISILEIYDGKELQFKQNFNEVTQIEFLIQNPAEKYIFIEQIEKGVNCKLYKIIDRQLNKGFVAKVFKFHENFNCYRLKREISFIQNIDCPCIVKYNETYLYSGCFFIIQEYMNFGNLRKLIRLFDKKIDESIIGYILYQIMLGLKYLHSIGKIHKHLNSKKVLINEIGEIKLLIMDYKIEYKKDKDPFWIAPETLEKQIIEEISDIWCLGIIAYELSERDPPYFNQHPIRVMYNIINLPSPKISKQRSNIFQDFIQLCLIKNYEKRTSLRQLMKHEFIIQNKDTGQQGLIQLMKKMNLNQKSKLKKSTSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TVP7</accession>
    <name>A0A8S1TVP7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ATP synthase alpha subunit C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0280082</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8156004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="9805536at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18113">
        <property type="entry name" value="ATP-synt_F1_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.150.20:FF:000001">
        <property type="entry name" value="ATP synthase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000793">
        <property type="entry name" value="ATP_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005294">
        <property type="entry name" value="ATP_synth_F1_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48082">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48082:SF2">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00306">
        <property type="entry name" value="ATP-synt_ab_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0139">CF(1)</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATP synthase alpha subunit C-terminal" evidence="9">
        <location>
            <begin position="366"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="196"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="279"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8156004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="501" mass="57682" checksum="CF9EA5CF7AA34DFD" modified="2022-10-12" version="1">MNKTGTGFKTLKLRSSHSFRPQSAYQEQIVSIIPYEMSEQAYQTSLKMDLLNIEDEISKLNKLGIQQSRLSSTHTQKIKQWISNIKMNDKCLDPQRVIQICNCSDANETVIKLYNMIQNFDFYVNKITELQCIHYLQYEQCFTNFQSNYFQKIASQGILPNRFLMSLLNLEIIEYLVQKKGDMNQSNGENILQKKLYDLKDENIKLLNEIKSLQSQCKKLQDQLSNSTTLSQSQLSHITVELPPNPASYYNKIIDEVKDSYQNLITMLRDENLQFGLKLSEVQQKYTNASNEIIELNKKVDNMKNQKDDLYKRFVAKYKTTEEFEQSYEAALKEEYSSMEISFKQKIQQXAINVGLSVSRVGSAAQIKAMKTVAGRLKLELAQYREVAAFAQFGSDLDAATQQLLNRGAQLTELLKQKQYVPMCAEEQVCVIYAGVRGFLDKVQTSEIAKFEEKFLTHLRTNFPAMLERIRSTGELSKQDDAELKSILEVFIPEAGLAMKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TWQ7</accession>
    <name>A0A8S1TWQ7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0290045</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TWQ7"/>
    <dbReference type="OrthoDB" id="292895at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001088">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="18"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="304"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8156540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="416" mass="47531" checksum="26BE514647223DB2" modified="2022-10-12" version="1">MKKLPSTYLEPKIFDNKYIIKNQLSSGSFGVVYLAIHKITREEVAVKLEKGEHETLDREVYLLTKLQGIQGITKLYWFGAEQNYNVMVVELLGKDLGYYNRTYKQLSLKTGLQLLDQLLSIFKSVHQRGIVHRDLKPENIMMGKQNTSIVYLVDFGVSKLILDKGKHISFKDRKSFIGTTRYASIAAHKGFEISRKDDLESMIKLPWQNLQNIGDKDRTIAVGEVKMSTTIETLCKELPIQFADYLRYLKNLGFEDQPDYDMLKSLMKQCSNPNTYDNQFEWSDKQQNKDSVLEVKVQGSFLAVPGNDGAQRSNTKKQSNISNLGSSSSNVVKYVPSFQDAVQLKQSSQAPIKIAASQPHLDFNLHSETVDFDDIEENMSNHPTLEQKYLNLSGFDAKFKDEKMKFSRSAINVFDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TXL0</accession>
    <name>A0A8S1TXL0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0290263</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TXL0"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="96"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8156950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="386" mass="45379" checksum="DD312200994D1B66" modified="2022-10-12" version="1">MYCTRKHLLFDKSYIITLNQDSMNIGESTQYTKYSIPLKLQSLIDWKISQEDFQLVAFSINWKNQQRYFYADQNVLLRVKNYLAGRVTFKNIDYFYKQIGKIETKDQTIIYTMQGIDNNIVYVCKELIKEKQYSYQLFQNEVQTLSQLTHQNIIKLIEAYTNETSYLIVLEYLKGGTLSQCLKYCRMSINEVEVITKQILEALCYLHGKGFVHRDIKPDNILFCELGQFQNLKLIDFGISCQIIDLQNDLQMNFGTPGYIAPEILYRKNKKRISQKIDVFSCGAIIYYMLTGQKLISGSNQQELYQNNKFFTLNNQILKNISSQNYRDLISKMITEDPDQRIDAGEALNYFKLMKIPTNTSLSTQLNYQHDHIQEIPNFRKLITKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UBT1</accession>
    <name>A0A8S1UBT1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0360174</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UBT1"/>
    <dbReference type="OrthoDB" id="307182at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="381"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8161413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="514" mass="59989" checksum="5E6EEAB370D9A7BC" modified="2022-10-12" version="1">MIAKIVQKLRSLTMKSQIILFNVLTLVIVLQVVWINYYVQTQIFSSISFSSLYQILQKQDQDKIGFIAKNVALIIELKFLSILTQMEQIKEFYIFFEKEKENIVNNYKMDSCISIEDYINDNTSLYTPKFCYQAIGVPDYVTIPQNETGIQILHDFISFLNHYSFSLDVSFSGMIQISSVSKTKYFSQFPFCFLLPWFDITSLPWYQNHLLKTSENEKEGFIFSPLNDFFITKIKEMSITSSLYSDGNMIGIIREGLIISDYLIPTVPYNVLLLDQDGLVVYFNIEQLRNKTDYFYIYDQNVTGFNKTDWEGMIQFSSQREKVILVLENKLQDEKVNVYSLQVLKNNFTLIVYTNITTKIDSQNQSTAIRETSFINFTISLFGQILLGFVAIILQIFVLLIVFKPLKQFNSIIKQYTLSQGNNVNSEIFKMIHKKTQESDALTTLQNKILNFSQILSERQGKKCDMCKIWESFAFNEKELQLDIDQIKNQFQILQNGMQEFEPKMLKIIKQGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UCJ9</accession>
    <name>A0A8S1UCJ9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0380330</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1668162at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051425">
        <property type="entry name" value="Formin_Homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014768">
        <property type="entry name" value="GBD/FH3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725:SF1">
        <property type="entry name" value="DISHEVELLED ASSOCIATED ACTIVATOR OF MORPHOGENESIS, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725">
        <property type="entry name" value="FORMIN HOMOLOGY 2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51232">
        <property type="entry name" value="GBD_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GBD/FH3" evidence="3">
        <location>
            <begin position="39"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="FH2" evidence="4">
        <location>
            <begin position="889"/>
            <end position="1278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="799"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1273"/>
            <end position="1302"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="478"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="803"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="832"/>
            <end position="883"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8162961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1345" mass="156375" checksum="1E33E46A9A7B7B44" modified="2022-10-12" version="1">MGNAISNGKLISPISIDAQIKESQLTIRFVLDQKKLQTQKNTIPKDFDHQISQLIKELKVEPKLLQQFQNTPQNFKCLILNEYKNIIGKNLLQNLPNDEINYFTQKLINPTLQDLEQIRRYLKNLQIHNQQDQLEQFNNVINLLIENLFRFYQQTQQIIQKKKPPCYLYQIEILNILEILVKIEKNQQQMLLIPDSMDIILKNLHPTHVELTTIVLEIASHLCWHTDEGYNWVLKSLNKLYEDNEIIFFINTLQKSKNAVMIATICAFIVTLTESPTQEEERKRMCQQFIKSGIFDIYKMITNNIEEFEYKADELRFEVVLQQIIEQNKQKHQILTDPNYIEEITLPMLYQRESRKIYDIGEFSNSIKIIEGQIEAFLENNKDIHIQQIQEPKKQEVKQHAKHLKTTFLTPQYLNLIQDEMGKANLTNLQRAVENIKHESKIRGTQIVTDFAEGISKYIDKLHLPTQKENENHEISQFESMKQLIEDLHEKIISLENEIKDLNFKKQSNESFVDVLQRKIGDLEKKMKSDQSILSQLNEQLASKNKDFRALQQENEQQQKTIQQLENELNQLREKLSIMQLAKAQKMEFEIPPQRLSYKSDNQDDLRQQLDQLKQELQQQNQKFFNQENEIKKFQKLLKEQNQNLLTKEILLTEKNKECRELQTINEKLIKKKYAKKVELQQLREKIQSVNYVKTQATKIQSELKTIEQYQNEVIRLNGLIEEQAFYLEQQKKQTQKFDEMRENQKVLELKNYKIEDEYKKQSINSQILQEKLQQLQLEKDNLEKKLQNQQLNAQNSIQSNNTFQSPNPILPGQNQINSLTPPISSNSQTGPAPPPPPPPPGVKTVSAPPPPPPPGGPKPPGPPPPLGGAPPPPPGPRPPGGPDPQFGGKQKHKPNVQLKQVPWTIIKPEQIKNTIWEQIDDTKLKLDYSIIENLFAVKPTSSNINTQSGINQAQKAAKVSMLGPERIKNLEIVLGKLKMSNQLIVDSLYQLDETVLRPNIVESLITAMPNDTEVGLWQDQDQSNLALPDIFCINISSVKGYDFRLLSLKFKYNYKELVEDLQSKIIIFQNLIGKTKNDKNTKVVMEYALAAGNYMNGQSARGGAYGFKFEMMEKLADVKSTDNKSNLLMFIIQKAEEDLKEELVLVDENIDDIELAAKTPLNQLNADLSELKKYSKNVDKAIKSKVSHSIQDLVEEKLQNFYQQVISDLADFENLLKQLENQYDDLSNYYAESKVTFDKFFEKFYKYKIVLRQAKQNITKIKQAEQKEQEKKKRIEQQQQQQGESQIKKKQEDNSLSGLKKLDKDQILNEVAKRRESKKNSLMLLSNVSVKQQIQPSQTETQDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UDV0</accession>
    <name>A0A8S1UDV0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0370089</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8161909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UDV0"/>
    <dbReference type="OrthoDB" id="2098626at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="107"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8161909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="256" mass="30654" checksum="027C7EB01FBD7870" modified="2022-10-12" version="1">MLFSQQQDYNPRDIYQPSSMSYGAGIFKPQNQQPYHNQQPRTRAERDYLKEVNSQQAVMQSRQFDTPQKEDMKALLHSPAPNTTLFQQEQERFLKDFAAQDKQQREYQNKQKQLQYESRRLANLEREGMIWQRNELHQQKDELKRQYHQEQFTQGKRNLNGLAYNPLTLEYAQNEQGQALKRQDEMTQVRQLVRAQNLDNRANCGYNLLTGENRKGVEEIVPDDLRGHYQNKMQERDNQLNIKHYALQQQLLSKQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UH15</accession>
    <name>A0A8S1UH15_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0260037</name>
        <name evidence="4" type="ORF">PPENT_87.1.T0400321</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8154576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8164298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UH15"/>
    <dbReference type="OrthoDB" id="298993at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036273329" evidence="2">
        <location>
            <begin position="17"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="231"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8154576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8164298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="359" mass="40153" checksum="0AFBA292CCE934A0" modified="2022-10-12" version="1">MQKIALLALVLAVTAAKFIDTHTTLAQINADPFGHVVLSAIKAHLQASTPANEVNMLLNGVGAGLVQDQNDHDHAFELDTTTNNRIVEDLEKEILYHQNQIASNTQLRDDTIEALAVSEEDIRVTIQDIANNEATYAREEATRNQQHETFVAKVAAIDDVIDAIDEAAKLIQHLSLGASFAQLKSKYDVLHKKLSDNTSHTALLQPVITALTELATHGVNQKALTKIAQLLSEIRQQLVQEKAAKTDVEDRQAAHWAEFSVHLSNEHTRLVERKAQLEVQIQEQKDTIEDAQSWIEFHTLELENSEERLAGQQAWYAVQSEIYETQTAERTAQQEIVDRLQEHISEKLSTTAQFIASRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UK06</accession>
    <name>A0A8S1UK06_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0420193</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UK06"/>
    <dbReference type="OrthoDB" id="296869at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398:SF0">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="330"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8165398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="619" mass="72580" checksum="B70E0FD05852C72F" modified="2022-10-12" version="1">MMMKKSQGFFKNLDIFGQNIQLNFNGENQYQTAVGGLFSIAIIAVIGFFFQQNIVNFLNRNEINLNTQTIFDSNPDRIELNDNNYMFAIAVEQPQFNTFPFFNITIKQRIYTRSSNGTIQKNDTFIDLIPCTQDRFQNIFKNQNFTPQFTSLKLQEWLCPQHNYSIILSGGYTSDLFEFVKIAVSDCSNDTASNTILSWKPQCSSSQNRDLYLQQERSFRIKMYMTNNVINPLQVQNMSQVFLDDESFFSFLIQTGTETDVFYQKYNITTDDNVFPYLKEIQENVYNVKKAGDFKTSTVQNNGQQYSAIYMRRSPYTFVIKRDFQDIADLLSYLGGFANIVALIFGILIKSYNKSRFMIDLANQIYDFPIKNDTPEENQKRKEIKKTIARAKSRTIIIKPQDQDLQSQLQSPKYTQNQQLILNDTIKKEEQFISKQDEIYPAKSEFIITHRQEEQRLFQQEQEQIIKQLGINDRKGYLTQQILKILNRSKPILFNYKYILSKIFCTKFFYDRNCILLQKAIQKINKDLDLCVIIDKVKEINLLKELLLTQDQLILFDFAPKQTINLLEEQQNMTRSLVRRTLDSLRSNSTSVESFNQDDSLNAYYKLFQVTITIYNIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UNW7</accession>
    <name>A0A8S1UNW7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0430285</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8166104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UNW7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8166104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="406" mass="47849" checksum="BFACCB95D8525D86" modified="2022-10-12" version="1">MYQITKQMVDFRRNQFLNQGLQTEFTIQSENGNKLVSVGSQLKNLNNIEKHRYLKLDSSFEESSGESSVMCDQFQQKFLSIEEVQQMVIDTSINQAIKLEPKALQKNNRVGLEWESQKSIIIMNVDEQAINEIDLYDIHFYELRFKDKSFIQFKKIWIGSYEGKLAIIIRMKEDKSAQYLYYNLVTSTHKYKKVFGEQIEVSLFINTQYFQLKVIEKLPNNNEISVIMRFFPIQFNNEKLLSLQDMRAEQLFIYQGYKYGIFKTNDFEKAENYKKQLISKKYQEAHISILGKKKQSNLNYKAFFEDDCFNPRFQLEQKIGMPLSPPKITPQNIIRQQEQQLITVAVIQPTISAIKISEQESSSKEYKQSEVKPKPVEKMEQKIIAKPIQLKPVAIQIERERSSRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UR49</accession>
    <name>A0A8S1UR49_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0440060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UR49"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8166272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="115" mass="13219" checksum="1B25DF6954EF579F" modified="2022-10-12" version="1">MYFHKYNNGYTFIKINNLMISNNYATSIQSDFNLKTQNLASILLQSDVVNITNLNILRGYGLKNIIIQNPKVLRIESSIITQSDEHKFLVFINILITNYNKSKVSTIYNGSLQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UTV8</accession>
    <name>A0A8S1UTV8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0490236</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UTV8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035802640" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8168678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="153" mass="18632" checksum="5245BF0B2880765D" modified="2022-10-12" version="1">MSLYSFINILLIICGLNLYNQKFNFDRDQQKEGFEPQKQQQRTFQIVLLMKTLKQLLYQHVAQIGIELKQQYNFDEGRWIIYLKMISINRVKQKFQKASNLKEMNQNKNIKRTKLRRLNTERLKNTSIKINDIEEQYDDIIFLYFITKQPLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UUQ0</accession>
    <name>A0A8S1UUQ0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rhomboid-like protease</fullName>
            <ecNumber evidence="6">3.4.21.105</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0490015</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Serine protease involved in intramembrane proteolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.</text>
            <dbReference type="EC" id="3.4.21.105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peptidase S54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.105" evidence="6"/>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UUQ0"/>
    <dbReference type="OrthoDB" id="312200at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002610">
        <property type="entry name" value="Peptidase_S54_rhomboid-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22936:SF69">
        <property type="entry name" value="RHOMBOID-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22936">
        <property type="entry name" value="RHOMBOID-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="7">
        <location>
            <begin position="80"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8168295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="263" mass="30225" checksum="28DD269AE60142CA" modified="2022-10-12" version="1">MIYNPINQEQTFFERFSFKSLTFKTTVVQFIIFFLTIFHTIYLIEKYLEIDEDESDIYSFKCVLYTWGSKHTPSIIYRYQFWRLVCPIFLHGSFSHIIGNMVVQIYYGFILELTHGWKRVSILYIVGGIGASLFSCVRFHYETSVGASGSIFALLALELIYFITAFPGIEPKRIVVFILLAPMIFLSFIDSPPQVDIAGHLGGLVVGLLIGIGYAFADAQSNIKQQCQYIFYGLVGLLFIYIFTILYQTKLEDEKICQGMNII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UVE4</accession>
    <name>A0A8S1UVE4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0480079</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000048">
        <property type="protein sequence ID" value="CAD8167892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UVE4"/>
    <dbReference type="OrthoDB" id="310600at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021258">
        <property type="entry name" value="Epiplasmin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10992">
        <property type="entry name" value="Epiplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="287"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="315"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8167892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="324" mass="36880" checksum="33918C4A395D4353" modified="2022-10-12" version="1">MSNVPNSHHPPQAPQQGAPYQPTQFQPGFAPQYAPAPMTYGPPLTSSPLRYSQPLYQPSVVAQPVYAPQVVQQPVYAPPVVQQPVYTQPVVQQPVYTQQVVQQPVVTQSVVAQPVVAQPVVAAQPIKGESRIEYVPYEKTVLEYEEVRQKIQVPKERYVTDYYAVEYQTEYVPQVFQEKYTEYVPVDRYQERVEYYPVERQVVHQQVQQVQQVVAQPVVQQPVQVVQQPVQVVQQPVQVVQQPVQVVQQPVVQQPLVQSIPVQTVRPPVYAPGSLPLAQTVSPRLPPQAQTKPQTQQPQPQQKQPQQQQQQKPKSFLDRLFDRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UWK5</accession>
    <name>A0A8S1UWK5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0500128</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8169035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UWK5"/>
    <dbReference type="OrthoDB" id="307600at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="197"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="37"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="210"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="233"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="253"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="286"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8169035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="305" mass="35626" checksum="39275618A5DEF310" modified="2022-10-12" version="1">MQSNTIKQLLLDIADIIDRDIIQTYQQIIQVSVKQVCEQSEKVKTVFEEQLEEHLNNDYEAVIQKLEASIRIHIRVEQQQKLQIDALLQKVDEITSEKDIIIKQQQEKIKSLEQVIKDQHKKLQDNSPKEIHHKKTPSAFERLSKLVSNKSQKSLQNSNNEANFKALLKICNTEKDRSLSKGRKQLSAKRECGYQRINEETQNLEPKMTATERQRDSKGEKRHRLQKSEKLNEQSQQSLSTEKLTKYNKSKKKESPLIRQKPEQTLSSQSFDQVGKSLQALQRQISNTRLQNNQSSQLLKFLQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V0F8</accession>
    <name>A0A8S1V0F8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA-directed RNA polymerase subunit beta</fullName>
            <ecNumber evidence="9">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PPENT_87.1.T0530278</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="9">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20 21">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="9"/>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V0F8"/>
    <dbReference type="OrthoDB" id="10248617at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1070.20:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1110.10:FF:000013">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1800.10:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007646">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007647">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04566">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04567">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase beta subunit protrusion" evidence="14">
        <location>
            <begin position="45"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="13">
        <location>
            <begin position="275"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="15">
        <location>
            <begin position="504"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="16">
        <location>
            <begin position="608"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="17">
        <location>
            <begin position="688"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="11">
        <location>
            <begin position="741"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="12">
        <location>
            <begin position="1111"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAD8170850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1214" mass="139422" checksum="A2FBF3C4A70CDA07" modified="2022-10-12" version="1">MSQVEEEDPRLFSDDSIQEEDEDENQKLDNDDIWKVLHMFFQTHGLVSQQINSYESFVENIIKYVRGHEGTFVVRVKPQYKEEERMDAQTEYLYQFQIVRCFMGKKNIHIDKFEEEIFPMIARLRDLTYSRQLKIDLEVTMQQRNKRDGSIEEKSKQTFKGLPFFKLPIMVRSRFCSLPKDPKDPEQIQQRIQSGECGFDQGGYFILRGSEKVIVAQERIANNVVLVFKSKIANKPWVAEIRSQPDLWSNPNNFKVELRYIQNTPVIRCSVKQFNSTQGIPLFTLFRALGISSDQEILERIVYNLEDEQMGPMLEMLYGSLQEGGEYEDEELCLRWIGNKIKKAENQDPETLIQEAKKLINKNILPHIGVSSESRDRKAYFIGYIVHRLLNASLGKTDQDDRDHYGKKRLDMAGAMMMGVFKTSFESFKQNAKKALEKYINGGRRGKGQQTRIENMRQPDDIKSFFDGEQISKDIDNALATGNWGRSKEGQVVKTGVAQTLKRETSLFATLSHLRRMNAPINPQMKLSKPRQLHNTHFGFICPAETPEGQKIGIVKNLSLMTAVSNDLQQKDKETLLKLIMVLKQDVFDFQLLQGDFQAQDIPKMTKVLVDGNWIGFTRNPEQFIQEFKNLRSAEESFIPIEVSINFDYVNKEIRIYTDAGRCMRPLFIVQDNQLKLKKSQLNQFTDWEQLLQNKCVELLDVEEEEGSLIAMDLNIMTRGTQNFKRYTHCEIHPCMMFGVCASVIPFANHNQGPRNTLQSAMGKQAMGLNSTNFNIRFDTLVHILHYPQKPLASSKAVDFITVNEVPIGINCVTSIACFTGYNQEDSIIINQYAIDRGFFRSVFYRTYKESEDKDPDNFTETKITKPVGDNYAGKLFIKLDSDGIVPPGTKVDEEEPIIGKELIIDNIMLNTANGQKNTKECSLLTRRAERGVVDSVLISENQKGYKLVKVKVRSLRIPQIGDKFCSRHGQKGTCGMTYRQEDLPFTLHGISPDLTINPHCIPSRMTIGHLIECLSSKLASIKGQFNDATPFAQISVNDIANELHKVGWQKWGNEVMTNPYSGNMFQIPIFVGPTYYQRLRHLVDDKMYARSRGPVTGITRQPTHGRSRKGGLRFGEMERDCIISHGTAKFLKERTYDVSDAFRVHVCSKCGLFAVANLENQEFYCNICKNTNQQNQIYQVLMPYAAKTLIQELISMNIAPRLKFDQSKLRHEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V0R8</accession>
    <name>A0A8S1V0R8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0520160</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000052">
        <property type="protein sequence ID" value="CAD8170113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V0R8"/>
    <dbReference type="OrthoDB" id="191686at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051480">
        <property type="term" value="P:regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051001">
        <property type="entry name" value="Calbindin_Ca-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19972">
        <property type="entry name" value="CALBINDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19972:SF10">
        <property type="entry name" value="CALBINDIN-32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="15"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="67"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="108"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="149"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8170113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="182" mass="21120" checksum="CD6D24C308C165EA" modified="2022-10-12" version="1">MDYRIGSASMSDLESAKIVAKKLFETYDRDRNGVIDTTETIPMIVDAYKSFNRSFQPTRGDIDSYSKVMDRNGDGKVTYQDIEELCIKYLCPQYSQQSRQQINKPQDPRIEVARRVFRKYDTDNSGFLNVQEIQKMITEAYRSMGLAQPTYKDVQAWLDVADTNKDGFISLQEFEQFVQKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V6N9</accession>
    <name>A0A8S1V6N9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0570132</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V6N9"/>
    <dbReference type="OrthoDB" id="3045089at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047126">
        <property type="entry name" value="RNF141-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109">
        <property type="entry name" value="RING FINGER PROTEIN 141-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109:SF5">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="145"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="295"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8172514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="362" mass="42274" checksum="19C7EFBDCC574DF1" modified="2022-10-12" version="1">MLLKYTEGKTQKKFDHLFHILLQIYVITIYYLGLNYYIFCNTFLILSFIVNIGCNKNSEDQYEHSTIIRLTIIFYRFCLIVSVLCITLKLNAYVNWTWAQAFWWYWMFLSGLVGTTITILLILISKIFSIYNRSEILNYKSEIKTLVWTLYISILSSLIAGIWIINTLNILEVDLNLKFGDCIMYILISFNVLMFSAISKYLFNDIVEFVLSLNQLESRDSSPNTPNQDQKIKKEPTKTQIFLQKFSSAYFKQIKDPEIVILKCTNQNEIDTERNTNNKIIQKSQDPQQQQDYKCIICCENPPNAVLMTCGHGGICYQCAIQMAQKNKECFLCRQNIKEIYEIAEKDVSILKRVISKTRISG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V8X6</accession>
    <name>A0A8S1V8X6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0580182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000058">
        <property type="protein sequence ID" value="CAD8173035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V8X6"/>
    <dbReference type="OrthoDB" id="284428at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8173035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="149" mass="18016" checksum="368DE9CE1F8E9BA2" modified="2022-10-12" version="1">MMQQRDFYGNDYIYKHIKQVSLQEFTDQLFNPVRHQKQMVLFTQNEQKEQQELYKYYVYLGGGVFTVSALSVIFYKKIPFLNRIEKRWARILTKALLLFGPLQVQSIFGQRAQYELLDKQFIQYHRQLQDYMFSGDIRKLKQDIQISPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VAH9</accession>
    <name>A0A8S1VAH9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0600282</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000060">
        <property type="protein sequence ID" value="CAD8174210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VAH9"/>
    <dbReference type="OrthoDB" id="306612at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8174210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="211" mass="24217" checksum="04F5A33903CC3C52" modified="2022-10-12" version="1">MLKLNQLNEHLDLNIKPNIAENNMRDSTSEVSNSNVCLNLSIQHQITQESQTQQFLQSQQTTQRSPLQNTDGLIYSNHELLQGEINQFSSLEDLSSKLNNYIDSYLQELRQPQQFLEQLSLNQSESYEEDSSESLCQRIYSKFVSQLNLSVIQSYDQISSIDEQNKDATPLSLSTPILKQNVEIPPEKSGSKQLFFQDHIHLTYSYPLSQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VB76</accession>
    <name>A0A8S1VB76_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0560111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000056">
        <property type="protein sequence ID" value="CAD8172146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VB76"/>
    <dbReference type="OrthoDB" id="322218at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="67"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8172146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="269" mass="33078" checksum="F5D535DA5093F365" modified="2022-10-12" version="1">MEMCKEDIMNLKGICLHHMMYKYYQYLNMSYKKYHKLSNQFQQQNSDLHKFSIQYQMCKQHSKQGRLNNLSQTYKTLEGKLSNYQKNYKNHIHQNKHHIYTLEKNNNLEKKYIKHYDKIVAYKSYMLRHLGNQRNQRDNPNRNLRFQLILDHNTKLLKYYQVSLHRQFIKIRKTVTMWSDIINKNIIQKKLMMQLGFYKHILRNSLQSINHQNQLQEIQINCNLQISTQNFFQFLTVVIIIRKTCLTDNNNYLYLQYSKILRIKQFFQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VDC1</accession>
    <name>A0A8S1VDC1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0610137</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VDC1"/>
    <dbReference type="OrthoDB" id="288106at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8174503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="128" mass="14579" checksum="F9A647B2AEA68181" modified="2022-10-12" version="1">MGNNCCSISISTAQTEEEIVSSNQQESITKSLGDSLQIANFKELIQDHCQFYTYQDGDILLTSRPISPRQQHVIIYLEPKIVAEEEIKFQQEGMKGILSARTYDQNPESSCSSINCKEKLQKKVQFRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VDY2</accession>
    <name>A0A8S1VDY2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0620161</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8175077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VDY2"/>
    <dbReference type="OrthoDB" id="289935at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035896536" evidence="2">
        <location>
            <begin position="22"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="58"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="261"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8175077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="368" mass="41477" checksum="7CB2EA4001FED361" modified="2022-10-12" version="1">MFKVAVCALLVLASTAINVQSSIWTSKDQKAFAQIHQSGWGKFILNFAELHLTTGGILSELNSEIEKLIGELEEELAGVHHEFNRRTDVHNREVARLEQEIQDKERELFNAHDFYDNVLIPQGERFAAQLEQLQENIAHNRQTLEQATVQRANDHETFEAQVAEHNDAISAIDECLQLLSTIETPSLAQVKKVQKNLAKIQSSLKKHSQFQIFVKVLLEITVDSNFADQGALRDIIVAFNNLRNELVDSLNQITADEADQVAEFNAQVIQLNQEHAEFQRAVVVKNAEIEANTTKQEQTLDLIDELDADLATLNGQLQAENDDYAFATDVYNATVAEYNKEINAANQALDLLNQPRFQDYVKSQLRGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VGN9</accession>
    <name>A0A8S1VGN9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0630109</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VGN9"/>
    <dbReference type="OrthoDB" id="309229at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="260"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8175545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="351" mass="42029" checksum="D1502147D4E6039D" modified="2022-10-12" version="1">MLITRIYILCLRELLGIQFMFGVSCPFNSLYLGTTCSLLLMILCIYQITVRVIEQRRIYKRKQLLFSIILASNIFFLIFTMNRDFIFAYFFCYYFDLCFLIFLVYFFTKKATDLNVYNKSKSKLVRGCTYGLFSLFSAFLFINLTVYYFWNSKCRIITIFVIKLVELLASIIFITGAYFLNKKMNALIIEQIMFTNRMTGQEKTQYIKTRNIKLKFWTLVCVTAFGQVIQLFSDLIYFIIDVDNNQDKCEFLSNNQLQNPLIDLFICLFGYFLPLFCAVYLFWYKKKKIQYVRNPQRWKAYQNVTITNCQTSRIAHSRLHKKIEITIKIIYNILQYYIIIKYLQLIKYICS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VLM6</accession>
    <name>A0A8S1VLM6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Anaphase-promoting complex subunit 4 long domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0690109</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VLM6"/>
    <dbReference type="OrthoDB" id="292688at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024790">
        <property type="entry name" value="APC4_long_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12896">
        <property type="entry name" value="ANAPC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Anaphase-promoting complex subunit 4 long" evidence="1">
        <location>
            <begin position="196"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8178304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="634" mass="74439" checksum="A2706119C44B4755" modified="2022-10-12" version="1">METQSFSPMLDRVMAQEIQSVSWSKTMDVCAILYVNNQLEICRVSEQLQRMFIIQEDDVISNIIVESMFLAYNVANKLYIYHLASHQKIIQSTLIKNSDKITQMLLVQVNAKIIKEQELQAAIIPLSTTNLKNWQNLCFLKQQQINNIIFSLTNKQIAYNFNGILHLGEIEHKSITQLFQLENELHCFSNSFVEIYDIGLLKHQQILKYIQQLSYLNELLCFLKTSILLINTQLQDIFKCYQLNIIGPLVAFGPQETINYHILQFYQKGNCPQELVQFFQKELYNTKILQKMDDNITNSFNNIQEIIQDSLMNVLSRILIILNFFIQSKNMPFYQGLHKTPFEELKQTIQNIQKVFSRFQIECHQTKLHLRNFFVWLNLCAIKAGNEQEVECENVNSPLNKIEVDLSKLFDFLKDNHLFQLKNLQCFYQEKIAPKTFFTKIVIQKNVEFKQTENITKILKNVFKDSYMDNIQQKQSKIEQEDTFVTVNSLVRQLQNYLDKIAINLKPQLINKINIDLSSSIIRFSEQSENSYMLSVLVNNSIIQYIYQLKSFQQLNQIQFNNKYIKELQYFDGKLIILSTNQQENQQQLIAYISSEDLETQNKFECHSIKDLQVSKKGLISIIIDSKKLIILSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VLT9</accession>
    <name>A0A8S1VLT9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Rab-like protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0690159</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VLT9"/>
    <dbReference type="OrthoDB" id="275177at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24073">
        <property type="entry name" value="DRAB5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08477">
        <property type="entry name" value="Roc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8178364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="191" mass="21559" checksum="6B5F50B4DCF2E1C2" modified="2022-10-12" version="1">MFKGSSIKILLIGPQQSGKSRLANYIADREDISQNGYRPTAGVRILEFEKEAPKNPKRPGSEKVIVELWDMSGDNKYDSCWPAVMKDAQGVICVYNAENPKHEQELEWWVNQISKKAGIPAAQNVVFAHHLTGKAIKGQSKLPKSLSSLTVHDTSIEEGNQTIHPAFEKFFNSLMLSIYEKQEKEENRLMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VR84</accession>
    <name>A0A8S1VR84_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0690035</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VR84"/>
    <dbReference type="OrthoDB" id="318635at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8178192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="502" mass="58002" checksum="24B30F19152214AC" modified="2022-10-12" version="1">MKLNFYLGFLHLFFAISQTTLSTIYTLLPGLDKTASWFINDFYGCQISNCKEYDIFGEFGVFGETKLPSLNLKLLRHYSLRISFKFWKIDYWNVDPFYLTEDNDEQTSQLFEGQGSNFCGSGGSQNKELPVPLIVTTEPQSLVFQMTSNLDEDTYYESYGFRDFIIEILECPEGFLFCQDYISDWKILNNIASYWLNSISSEGWLIDNNQQFSSSGSNILHQGQIMKKFIQNVPKHFKIKIILKLWVMGVKQNLNFIFKIDEQVQFININALSQQNIGCVNSQLVNINNITINNDHSSPEIRLEMYTENNQPQSRFWVVQSFDLYVAQCSIGCEDCNGGLETQCNNCFKKWGFLDGKCIPAPPLEYTNIRIYQSQGLKANFINSFQLYIEELDQTITEIGQKKLIIDKSITFCSFLISVECQEKIKIESLFRNCNQCDNAQISFSNYCLHESNIINFSVIFADTIQSEKELIINISQTKLEILQVIISNDIETEVHILKIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VSC0</accession>
    <name>A0A8S1VSC0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0700033</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000070">
        <property type="protein sequence ID" value="CAD8178642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VSC0"/>
    <dbReference type="OrthoDB" id="308594at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8178642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="99" mass="11561" checksum="7A6A3755B52B3FF1" modified="2022-10-12" version="1">MIQINENKLKINLIKKKPNLKIQTSMLPTVSESQIKSNQVFNNESEINSPEVRKFEALLLDSLTKTHRLSQKFQLFQPIMMNEGLDQHTRTKLQRPTNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VT70</accession>
    <name>A0A8S1VT70_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0730078</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000073">
        <property type="protein sequence ID" value="CAD8179951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="300532at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000793">
        <property type="term" value="C:condensed chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43941">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43941:SF1">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="213"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="72"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="242"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="334"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="424"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="766"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1007"/>
            <end position="1422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="213"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="225"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8179951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1447" mass="173294" checksum="75BFFF17B379E0F4" modified="2022-10-12" version="1">MDTNTIQSLYIPDPEEKIVYKNVRKPNLPATADIAYCEIRIAELMAEVEAQKEHSFKIQQQLDLYVTYEQSLIQYESKNSILQEENKQLSQLLQVKSRDLEQAQLKILELEQQIDVLREVDQELQRNIQLLLFKDREIQELNNKYNSLEQFLQQNKNWELINQDLRNQIINWKHDVELWKDRFTKSENERKRLLELREEGLKKYDLIENIDTQPHSPQVELNEQQTEKQKIEKSPENHSQLLQQTQVNLFEKEHQNNLLNNELNGLRQQQLEFQKRLSELTNQIGKLDEKDKEIARLNGLLQEKPKIQIVREEKIVTLENTQLDEEKIRLQGLLDESNIRLENSFNDIEQLNSRIQTLEDVNQKLQRDNRQIQRLQEQIDLLIIDKQNLQASIVQKDKVLNETLALLRQKTIQIQEYYAVEQELNIANEKINSLLKDLEDYRRQLGELEQLAQQVKKQEHICQEKDKVVKEQLLQIQQLKQNRNELQNELNDSKIRINLLTKYESRVKSQQNTIQSLRQELQDTLAGSEQWREKYNQNCSEISRLQRLLDVSQQRIIKLQEELKNFNNNFELGQIKIEGVPIIKRIEKVTIQDNNQIEKLQNQLISSELTNESLIRNRQSEIQSTQRELDALNQLLQQKRQESELWKNKYLTSEQQNTQLQNINDKYKQLVLRCEGQKATIQQLESKCLEQGQEIEQQRSKIFSLSIEVHQIDGYKIEKEQQKEKALQLYQELILSQSRVARLIGTINELQRQQNIKSQNQQGKLVQQKSDTVEKMRQKIVELEMEIDDTKDDQISELEQRIQILESELKKQLDINGQLVKDLQNVDRKKQDILSELQSLQTSESDTNQLRIAFNKKIKECEQLKTDLDKIQIVIQSYEQQIEKSRELDANQKNELQRLKIFNNNLNKQIASLLLHLNSLELTINTQRHKLDAYPEFEERINDQIAKINSLEKQNDELKLQNDILNAQITQLEQNIIKLQPLEQQVTLLKEIINNDNRDIGLWQTRFQDLENERAQQNEDYRKKIDQYKIELKDQQNLEMIIKQQQNDIEDLSQNNQKLVSNIRELQTQQDKLLRDLDNKDIEIKRLQQIEQEFRKLQDLFNQEIEKYQDLSDKYQQLQNQYDQTRREQEKLENKCAMMSSEIERLKVMLKNKNQELELNRQQIHQTEQLIDQLQPLTVENKRLFEQIQKQIQQIEELKQQIMLLQRQLDDKKKQLEQQQINEEQQQEEINRLLNVLRSKEDDIHQFRQVTKQYEIQLKDNKKEDDQWNDLQNELNKLTQHVNYLNEIIGQKNIELENMQKQNTQYQLQLLENEKLDYKILEKTVEAREKEVEDWRRRFQKLTQDQEKLYNEKLESNNKLAGQELEIERWKRKVTSNESELNLLRNIISQLNVEIERLSKSSEDLLQDNEGWREKYSKLQQQIPSMLELIPPITPGNSFRISDKPQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VTX6</accession>
    <name>A0A8S1VTX6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0730180</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000073">
        <property type="protein sequence ID" value="CAD8180147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VTX6"/>
    <dbReference type="OrthoDB" id="284525at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="2">
        <location>
            <begin position="53"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8180147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="242" mass="28762" checksum="D07356FFBBF7D6EA" modified="2022-10-12" version="1">MIKFFGIVIYIFFILSVVGPIYSFLTLQPKRAQEFWSRLAVKTLRLQFRYLPNSKQVQTNKRTILLFNHRTQGDFFIHDVVSQYSANFLARWMVAIAFPMLAIFQQLFTQSVWFFRRGGNDLNNFFKWIDNKFDKASRPNLLVYPEGHRMHESDKVGKMKTGMLQYAYDRKIDTQIIIVLGIEKAFNERKFHASLGPTQLSIKVDEIIKPDKFATFELFVEHIQAMFKKNFEETYDYQYKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VU06</accession>
    <name>A0A8S1VU06_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0710164</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000071">
        <property type="protein sequence ID" value="CAD8179269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="323984at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="82"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8179269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="144" mass="16830" checksum="A3E157A44CDA20B7" modified="2022-10-12" version="1" fragment="single">MNSEQIVVQYKNKVEECLQGFQQDLDCSYNRIDAYLDQLLLDPYPSVPIKNLNLALPIVQISIPSIQPLHNQLIQELKPIINSTIHKQIKLKQQNIDQLQQQLILLQNKPIQQDNQIIQHNHHQSNLKPFNYNLIQNNSIQQXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W4B4</accession>
    <name>A0A8S1W4B4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CSC1/OSCA1-like cytosolic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0800061</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W4B4"/>
    <dbReference type="OrthoDB" id="297739at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF114">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="298"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="344"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="388"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="434"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="563"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="627"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="667"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8183065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="761" mass="88372" checksum="5FA9238A06572D37" modified="2022-10-12" version="1">MEQPPESIFASIDNCFDNSIVFYFRILKWNFILMIIFAILHLPQLIFCITGNSIFNQFQLQLETFTVANIDISNETAFLIGVLGDAIGTLLYFLSLIYLRYNAINKDSQFKISSISPWSVEINGFPSATIDPEDLRSILQSNTHEIYLVRNFQGTLSIMKNKAFKERQIRLEKKKLEVFSKRLSQADMYVRQDFIRVLTDELKDITKELSEKFNIFLAPDDLESIKAFVVFKSKVDRDLFYEKYNQDCLIRNGIYLFQNQPQELKLRGLYTIRVTEAPDPNEIKWENMDYQEKNNIQIILMHLVVAIILLAPLIVFENMALKNARNNTVKCTEYCLDNDFTRTAYYIITGFWIFIADILGWIFLELIINKEKQIYLVQEQKVILIRIIVYLICYTLLVPILISWETIGNIVLVIQSYTSIEKSLIFSDDLDRKWIINHGLIFLWVSIFYTLKLIILSIVSGCRCIIGGIRDQEELQLIGSLLTDENDEERQRLTGQLKTPANNNTNNNQSHQTLFLLNLDESVNYNVQSRQNLDIKTGKSFRRDSPLINSNPTFKTGLRYAKLLFSIFLSTIISAGIPLLPLLGAIQIGLQYIYDKNMLLKYHCYNEKQQVKLQEQLKSVGIITLKFTHFFLLLHLIFSVLIYGYPYIYTQNGMGVSQNWNDASNKLLYKLSAVIPLTLLFIILLVALIIDLVFFGINNNWKIQSDQIGQDVSQNLEDHVSMLKEQGSAISYNLKHHHEFKDILFSQKQESIRATLNQFKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W5K0</accession>
    <name>A0A8S1W5K0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0830139</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000083">
        <property type="protein sequence ID" value="CAD8184590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W5K0"/>
    <dbReference type="OrthoDB" id="308839at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="125"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="75"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="219"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="126"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8184590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="396" mass="48010" checksum="35B7EB3A32E10A9E" modified="2022-10-12" version="1">MYKITPPSQQTYKGPKLQQSPIRQLQNNETLTEDVLQSSSEQYREQLEQMLDLKPSIRNFKQNNNNNKSDLWNTIKQIEKRMQEQEHNLQRVNNEQQEEINKLKSEIKQQKQQYEQEIEEIKYSSRIQSQKPQSSYHSYTPKNTREYSYRSVSNNNLEDLQEKNYLQQMIQDLRQQILVLQQKNYYTPIQNTDNIWEQKFKQLEKQKETELQMKDQHHIERIRNLETQLINQKQSYENELFAIRKILEEINKEDSLLRNQNQQFQKELNSYLNQFNILSQQIQQQNQQIQQYQIQIQQLSNQLQQQQPINYQNQNQQLLELANQLQALTHLLDKKNQECEYYKQRSIQNLQSQQQNPQIISYRQALTPTRQNTLQQSSDSIVFPTLNKQQILQQFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WH63</accession>
    <name>A0A8S1WH63_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0900168</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8188035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WH63"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="320"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8188035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="399" mass="47511" checksum="C1707949FC36EE98" modified="2022-10-12" version="1">MLSLSQHSLLVLNDDRYINYFQDQILFWQYIIPSSIDIKNATMKYCQMTYLVYFISNKVYRFNPYDKNLQEFEIFLNYQPQQIEICDQFIFVIDLQRITIYNNKDQVYQIQIKNYINITQLTILNNNQNGYNIYILDMQAGLLKIEWNKQSHTINQLDLIQNGVALGINNENNFYVAYKRQYKYIIAHYQIIDSQIQIVRKFQSKKIIKKIIVQQDYTLFLTNNKIDILYQNKIYKILKFGIKDISIIGNKIIGISTFDIFQYLYEPIPNYVQCFYQLNEILPQQLDYQLTFNQINITIQKKKIEFSVQLQIHKSDTMKIVIGFLVCGIVLTLILLYTFITLKQKNLLINEMENNLKKSKVKQVPLFKSLECAISPKGQHSKFQSFDQTSNFDLNKLRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WMS5</accession>
    <name>A0A8S1WMS5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0980035</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000098">
        <property type="protein sequence ID" value="CAD8191023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WMS5"/>
    <dbReference type="OrthoDB" id="306108at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8191023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="214" mass="24960" checksum="5B14CC3573FECAC3" modified="2022-10-12" version="1">MGTCASCNSKIEIVESDTLISKQSVFQKLKELNQNTKSTIFMTMRSNKKIDNENLADQSSQVSEINDEKSDIKDIHGSVKLRKRAYSFQEQRIQQVHFLINDQNDSAKVRCFRFTDPNIPRNLSNSSSSNSQFREFNAHFKINDKQIIESDESKSIKSILKKEPRQKSETKSVHFAKNMAISQIKIKSKSCHHKQHKKKKKILNQIQRYENYQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WN27</accession>
    <name>A0A8S1WN27_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0950135</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000095">
        <property type="protein sequence ID" value="CAD8190107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WN27"/>
    <dbReference type="OrthoDB" id="337735at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20558">
        <property type="entry name" value="CYCLIN_ScPCL7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012389">
        <property type="entry name" value="Cyclin_P/U"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF108">
        <property type="entry name" value="PROTEIN CNPPD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027110">
        <property type="entry name" value="PREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0195">Cyclin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR027110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8190107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="183" mass="21578" checksum="7D83913476C40E81" modified="2022-10-12" version="1">MQNLDSTKELTYEQQHELYNQQIADNKQFSSAMAYLLMDLCEEGDNDANNIQELESFNSPKVPSISIYDYICRILKQAQCSQECLIMGLILIDKLSQKWGRIIVKSNNVHRLYVVSVMLSAKFYDDRFFQNSYYAKVAGISHEEFNHLERVLVFLLDFKLIIDPLLFFTYRQRIITNYFDNPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WR59</accession>
    <name>A0A8S1WR59_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GPR180/TMEM145 transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0980162</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000098">
        <property type="protein sequence ID" value="CAD8191260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WR59"/>
    <dbReference type="OrthoDB" id="429400at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019236">
        <property type="term" value="P:response to pheromone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047831">
        <property type="entry name" value="GPR180/TMEM145"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019336">
        <property type="entry name" value="GPR180/TMEM145_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23252">
        <property type="entry name" value="INTIMAL THICKNESS RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23252:SF24">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 145"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10192">
        <property type="entry name" value="GPR180-TMEM145_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035839739" description="GPR180/TMEM145 transmembrane domain-containing protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="200"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="271"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="306"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="339"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="373"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="GPR180/TMEM145 transmembrane" evidence="3">
        <location>
            <begin position="172"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8191260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="417" mass="48886" checksum="62D03603C379277F" modified="2022-10-12" version="1">MIKYLFVLILLYLTQTKRIKQSYLIKSLKDWHYISKFGAETGEVFYSLTFRIKNADENDERTIPIQYELYLDEEWQNAIEKSECERNSVARRKETIWIPVKSGYEFKGSLTAKVRAHLWYFGFSDCEKQLRNSFPVENKVKLEVELHITNVGDTEFTLEQFGILQIIGVIAFIDVIMLIYNGNQILEKQKKYEEFSLPLFLLVITLLLETFSYSFLTIHLWIYSENGAGSILLQILSVIFQVASQFSLTMILVMLSWGWQINFSKFDNFELFLPISILIGFFQITIVGVSFIDYDSYYKDHSYEGWVGWLAALIYIGEFIYFMNGLVDTYNKQNTKIQQFILLLGLYGGVYFISFPVLQTVNLFTARYVRYKVMEIGTMLFRTSAILLLTRLFTSKKSIFAQISIENKSFLDRNKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WUJ2</accession>
    <name>A0A8S1WUJ2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1000153</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000100">
        <property type="protein sequence ID" value="CAD8192055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000090">
        <property type="entry name" value="Similarity to HELICASE MOT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001793">
        <property type="entry name" value="TATA-binding protein-associated factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044972">
        <property type="entry name" value="Mot1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36498">
        <property type="entry name" value="TATA-BINDING PROTEIN-ASSOCIATED FACTOR 172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36498:SF1">
        <property type="entry name" value="TATA-BINDING PROTEIN-ASSOCIATED FACTOR 172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="3">
        <location>
            <begin position="1177"/>
            <end position="1331"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="4">
        <location>
            <begin position="1531"/>
            <end position="1687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="215"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="216"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8192055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1741" mass="203548" checksum="D5BC3CC1747837AB" modified="2022-10-12" version="1">MEDKINKLFALFNDFKNQQLFSLALAQIVNVYNDYPACLYQILRKTNKLIYSRSQDPRLRLEHSQIAAAIIERTYKIYNERLEEYLLSSSNDSIPENLFHFEPILDQQVEDAMQQEQIRDYEEIKSNIKLKSEQIDFIHLINDGSAILGKDMKYLKRSQENKNQKLDDETLDIKQEEIMRLMGLSGNFNLSNVTRLLEFMQGDAEIPIDMHSSSDEIVESSSDDDAVGQYKRKNKTKNKQPETNLKKKPSTQKKNQNTKYCVINPFNVIYNRAVYELMNHKWQERHASALVLKSIVRQKGFLNLGFSYLIKGDEKEINGILRNSLIEYISDKNGQLNKLEYLLTRCCVLIVMDRFADYFGNQTIMIVREAACQTAITLLTDRNYNTNIWIHYPDSFMKFVLLLKDFIEEGQRVQIYEVSQSALFLVKGLLKDHLQVIFDNFSEMLLQVGQRDNEDEILELLADIWKEFLQLGLNQKYYNTVYSITMEQLKKQDDISFAGKSIFQFLGVLFHIGFKIQKQFQGEIDILKKFFFHRIVEVKEQFYKYLLAFSKQQTQKQNDHRTINFMKIIFQTGIFEEKPETFQTLMNILEQLSKHLEEEQLYEFIQFVHNFSTDHQFKKYFYLFDGMKRESIDLHYPNLSQQSQNPIDNQTYRMIKSAYLIKDINYNHQITVDLDPINFIYTFRNYINNKTDLNIEVDSTRQNGCCFDELDLKIQVSMQKLNILLKNYMQIEKEQEYLTIIQGFLKIYGDFAIAPAHSPKLRQLYPIILNQLKQLSQIDYEMDQESIQKLQDTTSDLVQNLQAFTKLSNELSLNFKVLTSDHTESLIQRIRIDKFHLIQDIASERLARQMSLNPNCCDLTSLYVKQSTQQTVQYILSQKNQLKRKDYGRKRLIWHLLKNQVDFQSWHLFFERLFLQQLNYIPNDLRFNQTLMTQTSKLIIKVEDLKYNLWGIGLIQNAYSLFIQNPEHSDFDKWAQYLLSKLLNYLPVLSNITDDDNNKIILDDQKGGILLEFDQTFKKVVILKNNLLKLCAKFIHFLSISKNINVFDQVIMQINQLLKRNENGMYEILNIILKEYPISLAPISGFLTLKAIKKLTAKDPNTAQAASKLFGFLVPILTIMDIPYSPITPQLINKFQKAKEFQLGFGKMTKVDYKLEGGIKDRSILRDYQWDGIRWLGFLIKYNLHAALCDDMGLGKTIQTLVVLANEVFKRKNENLVSLVVAPSTVVDHWYAETKKYISNAVLKPHIYDGVFQGNLIMVSYNQLLKLNQNFLNQEFYFLILDEAHILKNSKNKTSRVIRSLKAKHKIVLSGTPVQNHLLELWSLFDILLPNYLGDEEYFKRNFSKAFHTNIFSLTEDEILFDEQQIKTLRLLHKKVLPFIMRRTKQDVLPQLPAKIIGDYYCTLSEPLQSQIYQILESNSFSTDIEQQITKTQGEQKNKVCESVLKLLHKLRQALDHPILVRSVILPNKRPKKKVKTENIEMLAEQEINLLLDKQEKPESYNHSGKMIALKDILQQLGFQSTDDVPQQQTQSDQITIYTNFNKVLVFSRFRAALQLIAEQLLKAQFPGLQYLILDGSVPQTQRYPLVTKFNEDPDIRVLLLTTQVGGLGLNLSSANIVIMFDHDYNPVNDQQAMDRAHRIGQKNVVQVFRLIVKDTLEEKIMGIQRFKSAISKAIVNQDNASLKQMEKTDLLSMLESTAQGNNKEKNNEEEQIEKLSGPYSKILGQLKLDLLEQLNFDKEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WVX4</accession>
    <name>A0A8S1WVX4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1060071</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WVX4"/>
    <dbReference type="OrthoDB" id="5839at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044571">
        <property type="entry name" value="P4KG1-8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45800:SF11">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE-RELATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45800">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="7">
        <location>
            <begin position="27"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="493"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="493"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8194194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="568" mass="66501" checksum="760C6C75FDC191AE" modified="2022-10-12" version="1">MKKGQVKRKFESFGFSSSNPYDQRVRKELVLHIQNQVQIGNDFTLKLYSWMKISILKQKIHKATKLDLGDFRVLYKNQELTLNNEDLEDYGIKNHVSLKLMARKQGQQTYFINSLYNILEETRSTQEQFQNAKWAVEGAFMKGITPKLTDFGTQGTYILENQNHKPVAIFKPYDEEAFAPNNPRGMKGKMNSPGLRQGILSGEGVDREVAAYLIDQSSGHYHNVPITNYVQICHPTFHEAEEYKQFQHEKIPIKEGSFQLYIPHDDNVGNYGSGLYPSMEARKIAILDIRILNCDRNEENILVRKKKVPQANGQTRQSFDYFLIPIDHGYSFPDSFKICRDEVVWYHWGQMAQQFTQEEKVFIEKIDPDKDIQMIKEKVQLRDICLRNVKIATMLLKIGAAADLTIHDIAEMLYREDPDELSPIEKAVQTAEYNYNNNVRVPKGFFKEKIFSNNLIQKSLEFESSNLNNLERPQINQPLLKLQIIRDEEENKEINERENQNQHSDKKLIRVGSQQNIKTVKKPTEKEWNEEFYAHITYLLQQLVEHKLQEKVPSPRKYRPRYISEEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WZD1</accession>
    <name>A0A8S1WZD1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1070134</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000107">
        <property type="protein sequence ID" value="CAD8194732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WZD1"/>
    <dbReference type="OrthoDB" id="284414at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8194732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="266" mass="32610" checksum="D857E55BF8336B28" modified="2022-10-12" version="1">MDYMELKDLDFLKFVRNFYRLRMATNEEYDPPFHEAINYYANYLEQLSLDYWEDLLINYNIYNYHFQQSYQKINNSKQDIELKKFTNVQLFNLYDKLIEFYKLNIKSDFIIALSIWYPSIFSILSSQARKDLSDRRENLTLKYLSKELTNMQQKLKFEFSIHKIVDISHMLFYDVFFIQTLKQPMQAHHQLLDVIDDAFIQFFQEQYNLAEIQQNTQAKCLQITKKQQVDKEPLKKFKQDCLLTIMQMRMKNHEQYTKFIKHYLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X5W8</accession>
    <name>A0A8S1X5W8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1130025</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000113">
        <property type="protein sequence ID" value="CAD8196551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X5W8"/>
    <dbReference type="OrthoDB" id="298738at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="84"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="38"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="53"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="180"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8196551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="194" mass="23012" checksum="C78280E1E4E5BB08" modified="2022-10-12" version="1">MSSQNDEESDVQDVEIVDGYTSEYISQKSSQPPFQESDYLRDPDDNKSDNISHQESISNRQSLHQRQFFNQRTAEDEEIVKQCMEEASKINEKIMIQRKQLKDQFKEFQNTINAARSNLKDYDMPYQTAHFPLYLKTQEKFVILPAQNLNIRKGTVESFIKTNLENENSVKKAEQDRRVAFKQRRPYQKRKFES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X6T7</accession>
    <name>A0A8S1X6T7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1140122</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000114">
        <property type="protein sequence ID" value="CAD8197117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X6T7"/>
    <dbReference type="OrthoDB" id="10527451at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="363"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="441"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8197117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="985" mass="117269" checksum="E98FF5C3967C4925" modified="2022-10-12" version="1">MFDNQKITPQFIEQCLQTTDNYKQTVLIKQAVQNNQEHLFETICITIKEILVLNDPNPPFAKLQAVRLSKELMDIFNMTFVYLFEKHTIESFIQYSEFGDPNDQQRGRTIFQIDSDKTIRQIGISLSRFTSECINAWALFFPETPSGEPTKFIKLQQNMINKGFKFDTRDYYKKDFVKQHEKKQINQNGQTVQIPNSQKGVILERQKIYILSCQKLLDLVADICLKNQQWVPDLFIRYENEIIAKNNDVEMLVNQLKSINQGDLAKQIIEQAAKLKQLENDLISLNRATYLEFRRKYIQDPTKDGEKSIQYVGPVKIQEIQVLHQPSFALSSQIQQKTPNEEQLLSNQFLQTQKILIQEQSNVNLQKNDTVQLQKQLEEERNNCRYLQGQNEKLEYEKMQIQQQLSMIQQEFNNYKINSEKLIKQQKDEHNVEINNYIIERQKLYSEKQIMLSQIEQLNKQQINGIEQQNNQQITQLKREIENLKQNQEIKIKNLSIQIDQYQLKINEQQTNQENLNLQLNQYKAQLKQKQIEYQILEEKQNKESLVLQQQLIDAQKKFNEIQIQHVNKVEEFKQLDSIYQALRKENQLKNKNLESQQQTDQFRQSINIDNKNQTIQKQQQFEIDHLKKNNLFSVEENELNQNIQQIKQDLDTINKPKYQILQRPIFFKHFPQNLKFQNYQVPQRRRPITSENLDLIFPYKTPQEYYQNKKQKLRKGKEINFLQDFYDLSYLNQENQVFFKQRCLGSQGILFVNCWIELGLSYQTQVSQNKAYFSYGLYIKQIQKGKKDIKVDYLEVEKFQQFWASKQTFSRQLVQDSNDLIEILVESNVFNQQVLTLMISLSGINSFQISIPIYICQQINYSEIKIEEFKTKWKKKYTSIIRTSMLKLNKQIMKDINNFMKLIPKIIIINPNKIQDFEIGQSAIKLGGLGKFQDTEFLIKFEILPNDMIYIYISYKSDLNTNQKQKLEQILRGYALIFLENNYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X779</accession>
    <name>A0A8S1X779_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UDENN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1130089</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000113">
        <property type="protein sequence ID" value="CAD8196679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X779"/>
    <dbReference type="OrthoDB" id="285972at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051942">
        <property type="entry name" value="DENN_domain_containing_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15288">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15288:SF0">
        <property type="entry name" value="UDENN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00799">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="2">
        <location>
            <begin position="108"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8196679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="607" mass="71460" checksum="90A959E1CBEE9735" modified="2022-10-12" version="1">MNQECVECQKKDQKIKELKQKIAELEEQIETFGPEFSQVKGLDENSQKLLKENIQKAIGDLIGQDPNKLKTNQLKAPFQRTQTSNLTEFLRQPNIDEQHTYLGQRFFEHFLIIGPDLEELQQVNKHTNEIIVKPKILYQFPNVNEHLYDVVPIYTFPNGILAKKLETSVHNFNDKVLPELKQIIMQTLQLTKGDNEYSITIPREDIQDNSRMTVSEFVQESNPNVFRNIICFEINEFICLDPKTYGCWLCPIVHCFVSYYPFIKFFLKENANIWNQLKLERIEPCLSETSSHEVLKRDFIYMKNKLNTIIDRLFALRSIQVTYDQKYQLNEIQIQTPKKDSAQYLQFLWGCDQTFQNLSFKELIYLISHVLLSQRVILFCKKKSVLSHIMLTIISLMHPFNYKLPFCIFIPDELIDRLAAPCPYFFGVVGEYDNYCQRIEEAQQEDITMFDINTKRVYFSKNQSRDLKKCIAPIKRNSHLKQLYSVINNSRENAFKQIGKHKLQKLEKSGSSNQIFKENEKQYMQLLKDIAKSFQTFWHSTVTQHIPVDRKFIKTGSMAALNLQLLSEVMLSKCPKDSKRFIKELIQNPTFTGYLDELYEGIYQQNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X844</accession>
    <name>A0A8S1X844_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1100128</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000110">
        <property type="protein sequence ID" value="CAD8195766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X844"/>
    <dbReference type="OrthoDB" id="300604at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8195766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="442" mass="52171" checksum="8E6E492F42137C08" modified="2022-10-12" version="1">MITNHPAYPMKEIDKYYLQTKLPEYQTIPINNHQKKNHDYYNDNTHIWNNPHQAQSNTKIFTQLKEQQKRGEKRIKKMLELQQQDKLELQKNKIIYYNLARDEDLNRLQQRKMERGSLQNLSQQQNDVQVENIVLKPIFSRTALLGRPDHLWNRLNQQSQKHINILINPRILKMEEQGVKFISEKINKDLQIEQIKEDLRLKNMDQIKQQSTRRRILNLELEQKSGISPSRQSLQRVTTALTRQSKDVIESTLQQIEKQQQSQTIQQFHNQILTSSQQNNHTSRPYTVGSVLNQEFNSVSFNNCLTIKNEQTQIQSTNQPQTTTPVLMLSSQKSKSAYTLSHKHPLDRTEYKGLLLAQAVEQAESTFSKTLQANSSLLKETSIYLNKSKQQMIPKKRRQLKCSNLINNQKQNDSNDLKNVNNMLDSFERKLYRSRNFYEEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X898</accession>
    <name>A0A8S1X898_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T1120061</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000112">
        <property type="protein sequence ID" value="CAD8196306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X898"/>
    <dbReference type="OrthoDB" id="10252354at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="97"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="366"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="379"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="223"/>
            <end position="224"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="9">
        <location>
            <position position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8196306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="404" mass="46472" checksum="05A22342D22AE1E9" modified="2022-10-12" version="1">MMYEEYDDSPLQFIKSIRIYLNGDSSKYLDLKIAQNSNWSDIQTQLDQKFTKFKEMRLFTQQGVEIFQDDVKFFKDGQSFYASRGDEFDPHSPFSQYETIKKLGEGGFGCVTLARHRITGEQVAIKVIKMMGNAQDIELNFREAEVLRSLSHKNIVKVFNSYALKNTQMAVIMEYLEGGELAERLRQKGRFQEEEACKYFRQIVSAIAYCHQKNIVHRDLKMENLLFCSPDSDDLKAIDFGIAGLQCPTNNDHVNIGSLHYMAPEILCGKVTRVSTSVDIWAMGIILYKMLFGKVPFNGKSQQDIVTSIINKDLTLPPNNFSEEVINLITQMLERNHEQRPRITDVEYHPWVNSDIRTPQQKQMLLQIPSDGNIRKSRPSLSPRPRSKSPNPSGIVKHPNIFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X8W0</accession>
    <name>A0A8S1X8W0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4200 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1150106</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000115">
        <property type="protein sequence ID" value="CAD8197438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X8W0"/>
    <dbReference type="OrthoDB" id="298534at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051147">
        <property type="entry name" value="CFAP_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025252">
        <property type="entry name" value="DUF4200"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21683:SF3">
        <property type="entry name" value="CILIA AND FLAGELLA ASSOCIATED PROTEIN 100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21683">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 42 LIKE-2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13863">
        <property type="entry name" value="DUF4200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4200" evidence="4">
        <location>
            <begin position="115"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="461"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="132"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="305"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8197438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="510" mass="61285" checksum="3836D034A9300A25" modified="2022-10-12" version="1">MSEKLPQIHKVNKSDIQSPFRYPSDEEVFLFREQDRKKQEAQNNGDMHIWDKKTATNRTQLKHFKSYETQIDDKFKSKTAYNDKDKKIINEALQIIQERKKQRAKGVSKEPIVTLLEQKKEMFLVSKAHGIIEEEIDKLKLMSKDKKEALEQSLKMLETDNENFQKYLDTNKQQKLDAEQKADQEVKEKKAKESEIKSINIKITSLRAEKTRNEEAVNNYMDHKKFLDKLAPKEWHDAKEKKRQQLVNRLKEELIKSKGLTEKQFQQMVDNKKLDELEEFEDEYEMYFKQPSQLVEIFNDLEERNLFLIQTTQDAEQNLEELKTKFNKIKKSLDDKVFTLQQNKEQLKKNYENVCQQIKTLQLKGTEKNAKKDHKELRKQIIEVCENFPNEIQGVHDLQNKETLDILVQIELHLERQLKQIRSFRPDRVENFRQECERDWKVKQRDSNNLKLKQEEERRIKANQEKIMQPSKKKTGRQPMFRSKPFDRVIVQQEESVNNEELEDQKYFME</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XBS0</accession>
    <name>A0A8S1XBS0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Core Histone H2A/H2B/H3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T1190140</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the histone H2B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000119">
        <property type="protein sequence ID" value="CAD8198716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XBS0"/>
    <dbReference type="OrthoDB" id="305527at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000016">
        <property type="entry name" value="Histone H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055333">
        <property type="entry name" value="HISTONE_H2B_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00357">
        <property type="entry name" value="HISTONE_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="11">
        <location>
            <begin position="24"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8198716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="125" mass="14106" checksum="90F27E160375810E" modified="2022-10-12" version="1">MAPSKSPKADAAKKTVKTVKTKKGTDDKKKSRRRQETFALYIYKVLRQVHPEIGVSRKAMNIMNSFINDIFDRIALEASKLVRFNKRRTLSSREIQTAVKLLLPGELARHAISEGTKAVTKYTSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XIR8</accession>
    <name>A0A8S1XIR8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1250099</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000125">
        <property type="protein sequence ID" value="CAD8200724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XIR8"/>
    <dbReference type="OrthoDB" id="284527at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8200724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="122" mass="14290" checksum="592ACC3FE481D21C" modified="2022-10-12" version="1">MDSISTCLLLLNKKSNKESQCTKDELNNSIDLQFDSKVSLKERQIVKYLHEMGIQTKTDPVLKEQQYWNQRRIESNKKLEEFQRCPQLSFNSCVLRLLNRRESSFGNTFNKNQSILQTSLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XM61</accession>
    <name>A0A8S1XM61_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1290098</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000129">
        <property type="protein sequence ID" value="CAD8201927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XM61"/>
    <dbReference type="OrthoDB" id="427795at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050459">
        <property type="entry name" value="WD_repeat_RBAP46/RBAP48/MSI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22850">
        <property type="entry name" value="WD40 REPEAT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="113"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8201927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="425" mass="49271" checksum="BBF314E3698B1CF5" modified="2022-10-12" version="1">MNYLTPKQQKWRQIGPCITDFTYENNIDWPVTSCKWGPIVQESKEYIRQKVYFAIKTDGIYDEVTNIWKQTPCQLIVATVDIPQVKYQINHQVTFVFQQLQFYKNPHLKIRQIIVHPGDVNIIKCNTTQKLIATKSDNSNVLVWDVTKHKNQQNPKDPHAGIPEIYLMGHSQQGHSTALDWSQEYKLGSGGKDCKILLWDINDYQTRLSTSSIFTSKRELSNICGNDSIKLDKRTVLTGHQAEVVDMSFNKYQTDQLVSCCQNRQIICWDQRMDGGKCWSLDEVHKKDIHCVSWSQYDENYIASGSLDGSVHIIDIRKPIGIQEYVKEVDNLSQVYSLQFGPDRNHLTIGSEELYSVNFQTKETTFCYFGHKGSINDFDINEKSPWTYVSTCQEHEYFGGGCLHIYRLLDLVYLNEEEAYQQLNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XPZ7</accession>
    <name>A0A8S1XPZ7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1330065</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000133">
        <property type="protein sequence ID" value="CAD8203173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XPZ7"/>
    <dbReference type="OrthoDB" id="308112at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="469"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8203173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="517" mass="59386" checksum="9A021CAD7C0397C1" modified="2022-10-12" version="1">MLQMIKCILITSTMYITYAEMIYPILNKAYKTQWEDNVWINCETKSDIQPKKCYEGELDWQECYEVPKEGICLANYITTTQQDLLQFNITFNTSANNNTNQGQIVEIEINKNSLQLQLLNYSILEKVKIDSNIISLKILTSSLVLSDLSIYTPNHITHSQNELSVCLNEYSLFNYKCIPQSCFDQISNINSDTHISSISQIEQNTTNLLIKSTFKIPLIECLIPKIYLTKSIEDNTITTILSDEQVKLNENDNSILEYYLTPEQFQKCYHFETQQAIVYQCFIGVAITINKKPQYLLMLISQIEVQKSTQTYTNSIQIFEMPTSDTESTIVGPIIIFSNASNSIIDTKNNLIETTQNILDPNYQDYQITFLNGTITQNLTIIPLKLEDERQNGSIKELILSYDNNNFNENQNYNISISSSLTLNQTRRLLGQRNIIPQMQIQNSFDGTKVSQSTVTFNVNQQSSLQGGYLALIFIAVILCSLFFIIATIVLIEKVENKYFKIKQKEDKQIPTELQQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XQ22</accession>
    <name>A0A8S1XQ22_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1320045</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000132">
        <property type="protein sequence ID" value="CAD8202788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XQ22"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF75">
        <property type="entry name" value="CENTROMERE-ASSOCIATED PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 3" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="5">
        <location>
            <begin position="439"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="8"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="754"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="868"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1076"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="512"/>
            <end position="519"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8202788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1141" mass="134304" checksum="1A379951F8B8F144" modified="2022-10-12" version="1">MSLNNIFKQKQIERIKEQQLNREQREKKLEEIRKIKSVQFKARQANRVEMFEENKLSKLRETLKEGSYPILPITKHSFRDRYKEKELDGNILLRSQLSGKERARQTMLDFNQGHLATGEFNKDQPHKNHYKWLKVDDQSKQGNPMRFGKGERVETERVRGVIQNTTQKVDYVKKDLMMLTNPTWKQDDKSKWMDNNTFNIYNKKRFDPSSWEQLPINYKNQSNVFIEGFEVLGDPSRKRYKEQEHKRTEYIPTVKEDQIGTTLHISRSLRTFKADQLINEQSNFDEYSQNTKLKYYKSALIETKSIDHQIPYQHSNNFKSLDFKNLRPELKTKIFRNSLTTSTKAQTQNCNQTPLKTTQTKFETEQATTADYMQSKLSKIQEVSQNLIASDQKISINQKTLESENSKSKTQFNKQQSLDNLIKLKYPYHQENDETTKKILHEYFRIRPLNQKETSLINYIIRDQTLQIGEQKFTFDKILDSNTNQQEVYDEIGKPIIDQILQGFNATLLMYGQTSSGKTYTMIGDQQQPGIIKRTINDLFDAIDSSPTESEFRIKIQIVEVYKEKVKDLLDINKQDLKIREMANQGFYIQQITQLWVCDKEEIYNALQKSLVNRQVGYTNLNDMSSRSHLLFQMTVIMNNEIEGTSYTGQLIMADLAGSENASKAGTTGNALQEGAFINRSLLTLSNVINGLSDKQQHVPFRESNLTKLLWNGLSKNSMTSLIITCSPCISNESETLSTLRFGIRAKMIKTQPKVNKEVTIKELEIQNNKLTKELEDKNYIIDQLKKDDNKIKIIKFEELILHLRQENQLLKEQNSQYITELDMKSLDYQEIQTNLKNSLLESDRLQQSNLQYLKQIEQFQLNKDLEIHELNTQIEILKQQLQQKDESIQQLSKHLTNIQRIQTQNSQLQLHEHYVEKIHTQNIGDLLKEIDELKKKTFEEKSEIKYQNKVNESQILVFTKQIKNQESEISNLKEEVKNYQLQLMKNNDELLNLMKQNNKLQKDLENRVLKVLQLQEQYDYLLDQLNKLKNQLNYEEIGIYEENQKLHSQMQEINKKYLQEESLRRSNQLTSSQTLQAKLSRIQQLEQENYQLKLDIQNSQILKSQNNNNESVFISQYQSVNKGSMISKIVKKQQNQNDLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XSG3</accession>
    <name>A0A8S1XSG3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1340109</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000134">
        <property type="protein sequence ID" value="CAD8203658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XSG3"/>
    <dbReference type="OrthoDB" id="291052at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8203658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="136" mass="15820" checksum="A4AE19CE83CA77E5" modified="2022-10-12" version="1">MDQQDYHKIDPTIIEDPMLDDGYTRKFGAYKPKFHGTTPGYYPFECIDYFYVVLMTLFTWGICLPIWYGLIEAAKANSKQYAIVCAVVFVGFYVIQALATYYGGRMKKVIENQMIEDKLKEMQQKELEKQKLLAAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XWF0</accession>
    <name>A0A8S1XWF0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1410097</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000141">
        <property type="protein sequence ID" value="CAD8205673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XWF0"/>
    <dbReference type="OrthoDB" id="299579at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8205673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="167" mass="20016" checksum="4BD3A3701A69DB56" modified="2022-10-12" version="1">MQKLKDSTDEISAKYKLLRLALILEKQALKTPLNILKIYTNIKWRFNKTTDFVYQSNQWNEIRRNPHSQSSPNMLKKSLKHVVKEVQIKNYTERVHRSQIEGRRTSQEMEQLVELARVNSSKRNLQKKSVDLTKDVYQNIYEFGLLFILFGFIWSMYYVISQQLKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XXU6</accession>
    <name>A0A8S1XXU6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1430090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XXU6"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8206220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="212" mass="24158" checksum="E39B3F34509868DD" modified="2022-10-12" version="1">MPKGKQTKQEQVKQTEVVDKKIKKGKKAKANDEPAEQPASPPQSPIRKGKKQQQEKKPKATKERFNKLYYPETPNQQFPNQLQQNVINVIASFGTSAPLKDITNNLNLFYTNQIEEQVVHSFLAQAEETGIIYERSGQYHVAQELVPPQPQPKQVVSQNIEQEALPLQGIESAVKQHLDNDQIDFEDQIQNQVSEKKDVINDNDDNQNLFDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y184</accession>
    <name>A0A8S1Y184_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1430066</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y184"/>
    <dbReference type="OrthoDB" id="10249433at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052920">
        <property type="entry name" value="DNA-binding_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43358">
        <property type="entry name" value="ALPHA/BETA-HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43358:SF4">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="69"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8206172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="338" mass="38171" checksum="4F8137823B0ACFF1" modified="2022-10-12" version="1">MSQSCTEKLWKPIIRPPRHYYQLKDLGNQITMVIDTVTKRTDFEIVNKRKLTLQCSLFEPVRVQDRQHSCMIYLHGNSSSRVESLTILEYLIPYNIAVCGIDLSGSGHSEGVYISLGYYESQDVQSLIDYLRDHKPYITQIGLWGRSMGSVTAILSASQNEDIKVLVCDSPFSNLALLCKEIATSGYGVPGCCFNCFFCFVKSKIRKEANFNVDDLNIIQIVGNLSSQVSIAFLSANQDQLVNSKHSKQLHCVFKGTKLLKSFDGQHNSKRPNDIMKEVADFVISQLGKQSSDQPQSSRDTKLLLLHNQREKISFQYDEVHPIMTGGQISNNEQNITK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y1C7</accession>
    <name>A0A8S1Y1C7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1460030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000146">
        <property type="protein sequence ID" value="CAD8207057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y1C7"/>
    <dbReference type="OrthoDB" id="443651at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8207057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="245" mass="28400" checksum="AE84B010F166C233" modified="2022-10-12" version="1">MQSNFWWKTTKKEQIRLLLSAFLGAIICLLSTTIGNFILIEIRYNLIFSAYFGFTFIITGLYILIRTKIFMNNNTQWTLKQKYLVFFASVIIASGLICFCLYFDQKWHEGLNYFTKIPLYVILGVSLSSSICYLSIDFINLIFGLSQKVQYRTIVETPNQIISFMFISSLIGFLQGLLFSSLDIEDVENKTISFRLILFEELLLIPFILVLGSIGGFFNEYLRIKGSHLQSYTFEPIQDPFTDEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y1Q1</accession>
    <name>A0A8S1Y1Q1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1430126</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y1Q1"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF206">
        <property type="entry name" value="HSF-TYPE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="3">
        <location>
            <begin position="3"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8206292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="224" mass="27028" checksum="7E457D187E791440" modified="2022-10-12" version="1">MQKTSKFILDLYNILECEDFIPIINWTDCCKMFYISDNQKFQDIVMKQYFKTSNIQSFYRQLRLYGFKIKRNSNFQKEFHHKYFVKGQQQNLSKIQKGKSSTDEKYGEIYNVDQIIKENQDLKQQLNQMILFQKKIMKQFEIHQTIQKAIVNKMIKLTNLLNNERIHHTILNEKKIENFVNLIDGFQPELLVLIIRFLLKRIDPKTPINEIQYSPTPFPFNTQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y1X1</accession>
    <name>A0A8S1Y1X1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1470030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000147">
        <property type="protein sequence ID" value="CAD8207345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y1X1"/>
    <dbReference type="OrthoDB" id="307203at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="165"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="241"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="386"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8207345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="489" mass="57223" checksum="F498572873149243" modified="2022-10-12" version="1">MENQRIIKSIKSPSSLNSKQSTKYIQQPNVLFQQHPTQIVKKSAQQNIPPIPLKQVNNILQTRETPIFKQSQDERYSYTKDATCLMAKLDSLREIKSADSPIRNHVSPIPSTLQSVVVLSQNNAVKPQLLQQVACSSCVEPFKIYNNNNKNVNQSQQLSQTLPPKKNYESMIQTLMNEQELLVKQYNDKIKLLEQKTQELSQENNNLVEQNKLFLDQNLQLKNEATQFQLKQQQTNLTYSNEDTKKTVQFLEHELEQKKEELNQLIQNMNEILSINQKTEEKNNELSQQIKIILEQKEKEINAYFTQISMYKQTIQELEQKIVQLIKNDSLQQQTLKEAQTKIIQIEQSDKNKQNSQQQQQQKYNVKETFEYKQLVLELDSKCVTITEKENQIETLKIKNQMIQQQLAQQQQCQISIKDHQDALMQKERENDDLKSNLINKDEEIERLNQQLKFVRKEKAKLSINLMNAGMANLVMLSQGQYQQDEITV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y3D4</accession>
    <name>A0A8S1Y3D4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1430102</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y3D4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8206244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="166" mass="19740" checksum="B321421F2FA49CC2" modified="2022-10-12" version="1">MINISQVNSQVFIKQRKPQCSSLKMRYKERERAWNDRIIKSEQILHLRTKESLNKKRLPKLQNTTAIIVQKYINMQKNQQESIEIVECEQKIVETKLDQLRQLYKVYQSIQYNIRSILKQKSSCGRGTKSNEQKISPNTALYVCNYSQRELFQISFCFVKTELNSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y9U3</accession>
    <name>A0A8S1Y9U3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T1610021</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000161">
        <property type="protein sequence ID" value="CAD8210835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y9U3"/>
    <dbReference type="OrthoDB" id="1714095at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000060">
        <property type="entry name" value="Serine/threonine-protein kinase isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF288">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13540">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="RCC1" evidence="9">
        <location>
            <begin position="42"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="9">
        <location>
            <begin position="86"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="9">
        <location>
            <begin position="135"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="9">
        <location>
            <begin position="359"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="9">
        <location>
            <begin position="409"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="570"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="173"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="463"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8210835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="828" mass="94518" checksum="10C003CE982ABAD2" modified="2022-10-12" version="1">MRLNDLTESRKMLKQFKDKDVRPPRSYSDFSKSPRQLQLDTTQLYSWGLNTCGQLLKNNKNITPHYQGVTLCSAGENHTIIVNNKNEVIGFGDNQFGQLGYESNTQPKLTPIFKDQRITSIVCGAEHTFMLTAKGEVYSWGLNLKGQLGLGCFDNINQPTLVYGLLPFGNSNGKAREQQQGHKRMKSANEGDQSTSPGLKERSSSIDNVMEQLVMMKEKIKQKNPNDNQCQILLQNDEAVQQVACGALHTLILTNKNRIFSCGFGESYALGHQENQTISEFKLIQNLSHFKVEKISCGLAHSGCIIEGGKPYLWGYCKSNKDFYKQPTQIKMVDEFNNQQCVLDMKMGEMFTLFLTSKGEVYSMGDNIQGQLGNDLSGSDVPIKISGLPLISQISAGRNHSLALSADYKYVYGWGSNIYGQLTGQPSVNGKGIISPKLLFTVPETQRIVCGNFHSILLSPSYLEIILDENDNNNKEQDKLNEEVEKYRNEQEILKQQNIQLQQKYDRMKNELISKKKVKDQKVQTTESYIQRQIKKQQSKFDISALEISPERPIKHRIFQSSLDIDFNDIMLEKQISEGGYGVIYRAKWRETIVAVKMFKIDGMNENHIRDFLSECHAMEALRHPNIVMFLGACTKPPNLAIVLEYCQRGSLWQVIQNHDIHLTWEDRRKMALDAAKGVLYLHSFNPPILHRDLKSLNLLLDEAFRTKLADFGWTRTLSNYMTSKIGTYQWMAPEVIAGQVYTEKADVFSFGIILWEIAAREPPYRNITGLQVSLDVLNNDFRPTIPKKTPEVFTRLTKRCWDRDPEKRPSFKEIIKELEMMKFPQGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YCU5</accession>
    <name>A0A8S1YCU5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trichocyst matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1640060</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000164">
        <property type="protein sequence ID" value="CAD8211600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YCU5"/>
    <dbReference type="OrthoDB" id="296223at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035904540" description="Trichocyst matrix protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="119"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="196"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="286"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8211600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="393" mass="46400" checksum="F53104B65C4D2BE1" modified="2022-10-12" version="1">MKFYIALLILVVYSQQDNKQISPEDQTFLETLQQDQFGLNVLNSIQTEYQRDQDQKAQFNRIEHLLNKIEQQIAQDQQTDLELQSEASEQYYSQLLSLEEQIAKSQYDYSTLGAQIRLYQDESNRYDVLMDEKENIVNEYQSLLTQLDEIRQQEHDHFEIARDDSITVCSLLKRAKYLIKQLIPKTTVLVQQQSNLNQDYEQNTDFLQQINQLQKEAKETLQNNQPFLQIINQFVSVTTQTDAVVNTKRVNAIVNLLQQLYDQVDKTSRVQYNAEDDREQLHSRVKNQINDELHSINLQLATLQNQQDSINAQIVTESQNQKDEEKRLANLKQQLLDLSKCSDDAKVNYGVRLKERKEQLKLLRQIRKVMLNDFDAIRCYIEDYFNKHQEHQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YD23</accession>
    <name>A0A8S1YD23_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1560008</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000156">
        <property type="protein sequence ID" value="CAD8209742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YD23"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8209742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="34" mass="4203" checksum="DF4AF2F790FA1A37" modified="2022-10-12" version="1">MQDRQREFTIDFSDFQGSYLIYMEEAPAHNKDRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YDR3</accession>
    <name>A0A8S1YDR3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein kinase PLK</fullName>
            <ecNumber evidence="8">2.7.11.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Polo-like kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T1550066</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.21" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDO010000155">
        <property type="protein sequence ID" value="CAD8209604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YDR3"/>
    <dbReference type="OrthoDB" id="408964at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13118">
        <property type="entry name" value="POLO_box_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13117">
        <property type="entry name" value="POLO_box_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14099">
        <property type="entry name" value="STKc_PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001669">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.30:FF:000013">
        <property type="entry name" value="Serine/threonine-protein kinase PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.30:FF:000016">
        <property type="entry name" value="Serine/threonine-protein kinase PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000091">
        <property type="entry name" value="Serine/threonine-protein kinase PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033701">
        <property type="entry name" value="POLO_box_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033695">
        <property type="entry name" value="POLO_box_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000959">
        <property type="entry name" value="POLO_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00659">
        <property type="entry name" value="POLO_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50078">
        <property type="entry name" value="POLO_BOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="32"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="POLO box" evidence="11">
        <location>
            <begin position="438"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="POLO box" evidence="11">
        <location>
            <begin position="540"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="335"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="619"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="335"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="360"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="389"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="627"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8209604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="642" mass="72729" checksum="319A6AB909A06295" modified="2022-10-12" version="1">MDENKTADGSEQVVIFEEKITKVNGEVAIKRYQRGKFLGKGGFAKCYEATNLETKKVLAAKIIAKSSLTKNRARQKLISEIKIHKSLQNTNIVQFEHVFEDHENVYILLELCSNQTLNELIKRRKRLTEIEVQCYVAQIINALKYLHASNVIHRDLKLGNLFLNKSMELKLGDFGLATKLEFDGEKKRTICGTPNYIAPEVLDGKVGHSFEVDVWSLGVIIYAMLIGKPPFETPDVKTTYRKIRLNSYTFPEHVLISDAAKNLITRILNLDPVKRPTLDEIMAHPFMNTGGSIPKTLPLSTLACPPSASYNKQFQPSTNQSSLKMSVNAMPQRLTETTPNNQKNQQRPGNGSSDRFPLQKPSSSGNILEDNFGSSGLNNAQNAGYGGSQRPQSQKPNDIRSSQSQKTLNTPFGGTGMQGTHSVNNLGQRQPQQKQEIYVKKWVDYSSKYGLGYLLSNGATGVFFNDSTKIILDTKSLQFEYMERKGTDKQDFCESHNLNDYPRELQKKVTLLQHFRSYLEGEQNKQDMAFEEYDNKQLVYVKKWMKTRHAIMFRLSNKIVQVNFTDKTEIILSSEHKMVTYVNKTGERSHYPLATALDSQNTEMAKRLKYTKEILTHMLNGGHTGDQKPNQMTGTNWQNSGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YIP1</accession>
    <name>A0A8S1YIP1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1960003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000196">
        <property type="protein sequence ID" value="CAD8214095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YIP1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035875233" evidence="1">
        <location>
            <begin position="17"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8214095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="213" mass="22601" checksum="E9C23F5B80C30D22" modified="2022-10-12" version="1">MILLILLCLTPQQAMAQQCASTDPTICNAESNCGWWPPGNFCLKCSNQYTEGQCMDTCGWYNSVCTYCPSVPTASCIQSGCGLSTSSQCIHCGGISSSTCANYNGCYLNSEKCLSQRYSSSSNCLTPYQWVDSRCQIYACTSSKKSSCLCGTSNLVICSSTQVCVDISASTGVCQEKCNLNLNSNSCLCGGDSVECNSTTYCVDIAATSGVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YJ98</accession>
    <name>A0A8S1YJ98_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1930001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000193">
        <property type="protein sequence ID" value="CAD8214000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YJ98"/>
    <dbReference type="OrthoDB" id="312140at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="24"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8214000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="131" mass="14966" checksum="BD0030154A4751E5" modified="2022-10-12" version="1">MESTYQYNKITLTIYWAFYYPHCFSPDGNTLASGSDDKSIRLWDVKTGQEIKSSDKNYKDIFAQFKIPLQQQSHISEASNYITTLLISQSTIFQAQGALVLKGELINHQGTDLRPLFKSKGSCFLEDLKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YJF7</accession>
    <name>A0A8S1YJF7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1920008</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000192">
        <property type="protein sequence ID" value="CAD8213979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YJF7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="146" mass="17100" checksum="A2591495EED64E90" modified="2022-10-12" version="1">MDLSDIRAKISELEQRHNQVQCSLGSTFFQGAGNLPLSIPFIINRLTLVGGGPQCGDRRRNPQFVKAFYDFTENYQIHLLQEKYIDNLLAYLVKYYTNISCQYEFKLFSENNQLQLLDMKYCKLCSFLTNEPAVKLEYDVRHQKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YLP4</accession>
    <name>A0A8S1YLP4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T2340001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000234">
        <property type="protein sequence ID" value="CAD8214653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YLP4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8214653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="244" mass="28451" checksum="89077C48E19E9EF8" modified="2022-10-12" version="1">MLKQKKKKANLNGHENWVMQRNIQVFNSWDHSICLLGCKQGFQKPSKMVTSIWQIQFVSHLIDLIYHGKRKPNEMVNKIWQCESVSLLAAGCQHLVVVTRQSICMNGKQSEAVIIKKLTQFISPYINILSYCGADFSKNCTKKANLDGHIIMLDRCVFLIIEISHYDYSFRLWNVRVKIDFQSSYIDLSVYLEIQIQQLFAVLIYLSVNERCNQNNKKLKLIVVPIVSFLIYHQSSFWQCIVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RW00</accession>
    <name>A0A8S1RW00_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protoporphyrinogen oxidase</fullName>
            <ecNumber evidence="4">1.3.3.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0010170</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>protoporphyrinogen IX + 3 O2 = protoporphyrin IX + 3 H2O2</text>
            <dbReference type="Rhea" id="RHEA:25576"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:57307"/>
            <dbReference type="EC" id="1.3.3.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="4">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protoporphyrinogen/coproporphyrinogen oxidase family. Protoporphyrinogen oxidase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.3.4" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RW00"/>
    <dbReference type="OrthoDB" id="419752at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004729">
        <property type="term" value="F:oxygen-dependent protoporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000705">
        <property type="entry name" value="Protoporphyrinogen oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004572">
        <property type="entry name" value="Protoporphyrinogen_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050464">
        <property type="entry name" value="Zeta_carotene_desat/Oxidored"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00562">
        <property type="entry name" value="proto_IX_ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42923">
        <property type="entry name" value="PROTOPORPHYRINOGEN OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42923:SF3">
        <property type="entry name" value="PROTOPORPHYRINOGEN OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0274">FAD</keyword>
    <keyword evidence="1 4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="3 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="5">
        <location>
            <begin position="13"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8131572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="454" mass="52123" checksum="50C598A8E7DADE2B" modified="2022-10-12" version="1">MSHKFVIVGAGLSGLSNAFFIKHFFPKAQITLIEQSNRVGGMIVSNNRNGFICEEGPRSIRMGKYNRPLYHILNKIGLYKEFVPSIQQPYSYIYWNGKLNAVPLQMNLGTISQFIKDNGSGDLFKLIKAYPKIMLMNPNTDNLGEYLDHVLGKDLTEKYAESVFYGIYGESVYNLSKALCYTKSHMKGYDDQEISKDLSLDKDFEQVRDEKLQGANSYRFKNGVESLTKRLHDYLQETYKMEYKMQLNSKAKGIDLAKKELIVESREEQEEQRINYDYLILNAPTHQLSQIMANSNLNKIGEMFRGVKCNSLVTRNICWNQKILPKEFEGFGYLINPKQEQVILGMVADSLSFPSQYPENATNLSVMSIYDVNDHVILSELSKHLGVKIPEPKEIITKSWSKAFTRFSPGHQEEMKKIESELNTKNIIIGANNYTLAIPELVYLSYSKMKQLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RZ32</accession>
    <name>A0A8S1RZ32_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0010244</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RZ32"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="33"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8131704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="517" mass="59551" checksum="B68B71A6DC5A30F7" modified="2022-10-12" version="1">MANRLKAQIEFTDDAPVTQIDELEVSKRFEDYYQCLLTLGQGAHAIVKTAKKKNTEEIYAVKIVRSGDQEIQNNVKRTFNNTRCLRHPNIAQDIELYINEKMETSYLVMEYCKFPSLESIIKKRSLTSEELKVVIKQLLLGIQHAHSKGICHRDLKPDNILVNLEEDSQSPNVKIVDFGVSRRFLLKGQEIEMLTKTGNIFYCAPEIYHKASYSKEIDVWAIGVITYQCIFQKLPLYSNELHDFIELLANPNQWTFQEQLNTLEQPLSNLIMAMLNPNKSERISVEAALRHPFFEICTIKDLMAFLNKLNLEQDNCCRCRSLQSSLRLDDQQWGNVIQKLQNSQNDNKNDEIMLNELVKSFNDIHIIQRHGDKCGLIQLMNSINSSTALMSRLGSKQELQDKSFGFQFSGSSNKLSNQEQMDNIGKIQISIEMKDEQQNINNDNINHYNNNNKFLTQLGAHLDSSIDVMPYDGSLIQSKQIPQQDQQGITKKPLNSLKNMFGDLQIREVDEITEDQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S0H9</accession>
    <name>A0A8S1S0H9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0010529</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S0H9"/>
    <dbReference type="OrthoDB" id="409391at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051165">
        <property type="entry name" value="Multifunctional_ANK_Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123:SF141">
        <property type="entry name" value="ANKYRIN 2, ISOFORM U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="58"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="292"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="375"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="412"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="445"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="483"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="602"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8132274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="659" mass="76579" checksum="23C5867FBCDF7CDB" modified="2022-10-12" version="1">MDQDKDLLTQSLIFNIANNKQKEQEIFSFVIDGKLTSLENWMHQFNNVEEIINSKDSEGKTPLFYACYYNFKNIVMYLLVKGADPFLTSKSGFNVFHVCAQRGHFECLQILFQMFRHRQNILKWEELKITMKKYQFKKSDSQKGQLINADKHLKQVQQRFQQFQTIVSEQYQQFLDQNLQYFNRVNATIDQFQRNPIHYGSLSKYTKCFQCIKQLAQFDFESQGWDLFYDIFMEIQLLVQTSDNKIDPRQYKNYREMICNFLNQQLLQQKQTNFMSNIKKLQKEIVNQQDRDGYTPMHLASFAGDFAAIQFMLQLGADPKIKCKRKIRTPLEYASNDSVRKYLMDLNNAVKEGDDKSFTLLVNCGQRVNGKSTIYGITPVHKAIEQTHKSSNKTMLNKVLEMDADVNVIDTNGWTPLHHAAFYGELWAISTLIERGAYQQISSNKGYYPIHVAALNNQAMAVQLLIEKGVANQESFIDFQDNQNCTPMHLAAKKGHDQVVKVLFESGANIYSVDKRDWTPLHYASFYQNRNVVHLLSRYDADEDRYCAMRTSKGQTADQMTTDSEVKFAFKTLWSAARDGDLDTVRKLVTLNHPINEQSYGNKFTPLILATRGSHFLIVKYLLSENADKTLKDKYGNSALDYAEKQANQKIKELLQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S161</accession>
    <name>A0A8S1S161_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0010698</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S161"/>
    <dbReference type="OrthoDB" id="310100at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="23"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="189"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8132594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="319" mass="38354" checksum="F17BF1469C9419CF" modified="2022-10-12" version="1">METNRLRKSIKVELEHKFIRQKAEFLELVEKQLQMKQEKLKEQIEEDYQNLINANVKIAALEKKNDEKDKIIAEQEIIIRSYSATINNLQNQYQQHPQHKKLDFEEIEDHRLVEQLKNQIDDLKMKNLRLFLKLTKLLTFFTEFELYEKEFARIAVQDDCSYEQLYKPKLPNQQDLLNQLQQQEIHFQSITKQQQYKQLSEKNEELNQEMKELKKKLMRYDLTKTFDKEKQKKKISNLGEQIENQKNYIAGLIWQMQEKSNTTKCKTFNDILLKKRVLSTEGNDPVFDVNKSGRKVIVDRFKLSTLQCITQQRLHTLNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S192</accession>
    <name>A0A8S1S192_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0030060</name>
        <name evidence="3" type="ORF">PPENT_87.1.T0120037</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8133956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S192"/>
    <dbReference type="OrthoDB" id="289539at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8133956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8143097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="186" mass="21610" checksum="C0CB18763BFBE940" modified="2022-10-12" version="1">MDKLQQLIQEFFNVLEQNFFLSREFNKIFISQENAKQIAQEKKYQELTIKLLEFILSIHKSNQHSALQDRIAQTQKLLLNYHSQINQSQKKEIQPLNQNLDIPTVSNYKSNPVTPRGVQINQSKSVQNLHGPEKRQKSNQNPVQGMSKGTPNSSSQQRFFIEDIPKMGKSQVKDDQQRNYNIITGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S1S7</accession>
    <name>A0A8S1S1S7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Obg-like ATPase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0020104</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for GTP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. YchF/OLA1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8132854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S1S7"/>
    <dbReference type="OrthoDB" id="424823at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04867">
        <property type="entry name" value="TGS_YchF_OLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01900">
        <property type="entry name" value="YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.300:FF:000001">
        <property type="entry name" value="Ribosome-binding ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000001">
        <property type="entry name" value="Ribosome-binding ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00944">
        <property type="entry name" value="YchF_OLA1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004396">
        <property type="entry name" value="ATPase_YchF/OLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013029">
        <property type="entry name" value="YchF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041706">
        <property type="entry name" value="YchF_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00092">
        <property type="entry name" value="redox-regulated ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305">
        <property type="entry name" value="OBG GTPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305:SF11">
        <property type="entry name" value="OBG-LIKE ATPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06071">
        <property type="entry name" value="YchF-GTPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006641">
        <property type="entry name" value="CHP00092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="5">
        <location>
            <begin position="22"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="6">
        <location>
            <begin position="298"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="31"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8132854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="389" mass="44118" checksum="09C05B9111B735F0" modified="2022-10-12" version="1">MPPKKEQQEEKKNKLGRPSNTLKMGIVGMANVGKSTTFNTLCKLNVPAENYPFCTIDPNNAKVPVPDDRFIKLCQMHKPKSEVQAVLSIVDIAGLVPGAHKGEGLGNAFLSHIKECDGIYHVVRAFEDENVCHTELSVDPIRDMDIISTELLLKDLEFCNNRLAETEHVIKRNNNKDAREEKEVLDKVKQLLDNKKWVRTGDWNFKEIEILNKYYFITAKNVVYLVNLSQQDFMTRKNKWLKGIKDWVDGNCPGDIIPYSADLEKAIFEEFQANSLTQERQKLSMLPRIIKTGYKALDLIYFFTAGEDEVRCWTIRAGTKAPQAAGVIHTDFEKGFICAEVMKYEDFVSLGSITAVKAEGKYRQQGKEYVVEDGDICFFKFNVGGGGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S1Y0</accession>
    <name>A0A8S1S1Y0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein L19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0020482</name>
        <name evidence="8" type="ORF">PPENT_87.1.T0360223</name>
        <name evidence="9" type="ORF">PPENT_87.1.T0380177</name>
        <name evidence="10" type="ORF">PPENT_87.1.T0490209</name>
    </gene>
    <organism evidence="7 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S1Y0"/>
    <dbReference type="OrthoDB" id="304986at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.240:FF:000001">
        <property type="entry name" value="Ribosomal protein L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1650.10:FF:000001">
        <property type="entry name" value="Ribosomal protein L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039547">
        <property type="entry name" value="Ribosomal_eL19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023638">
        <property type="entry name" value="Ribosomal_eL19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000196">
        <property type="entry name" value="Ribosomal_eL19_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057259">
        <property type="entry name" value="Ribosomal_L19e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057260">
        <property type="entry name" value="Ribosomal_L19e_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006343">
        <property type="entry name" value="PRK08570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01280">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25476">
        <property type="entry name" value="Ribosomal_L19e_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01416">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00526">
        <property type="entry name" value="RIBOSOMAL_L19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL19" evidence="6">
        <location>
            <begin position="3"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="149"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8133592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8161511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8162663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8168624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="181" mass="21753" checksum="ECFDB077D2210740" modified="2022-10-12" version="1">MVNLRLQKRLASTQLKVGINRVWLDPNEASEISLANSRMSIRKLIKDGLIMRRLRTIHSRARARRYLEAKRRGRHTGTGKRRGTREARMPTKVLWIRRQRVLRRLLRKYRAAKKIDRQQYHEFYLASKGNQYKNKKVLIEAIHETKQEKVRVDKIEKEQNDRREKNKAQRTKKTQNKFAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S216</accession>
    <name>A0A8S1S216_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Autophagy-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0040195</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATG8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S216"/>
    <dbReference type="OrthoDB" id="6738456at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000474">
        <property type="entry name" value="Autophagy-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004241">
        <property type="entry name" value="Atg8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10969">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEINS 1A/1B LIGHT CHAIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02991">
        <property type="entry name" value="ATG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0072">Autophagy</keyword>
    <keyword evidence="1" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="13"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="lipid moiety-binding region" description="Phosphatidylserine amidated glycine; alternate" evidence="1">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR604241-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8135381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="130" mass="15633" checksum="E3B9B00BA4A1DE89" modified="2022-10-12" version="1">MNNQSQDNTKTQNMKDYKAQHKLEDRKKRVQQYREKYPEMTPMVVQKHPKAKIMSLTRPQFLVNQTVKFSDFKTQIRAKLQLSPQQTLFFYCGNNIISDDISLQELYNKYKDKEDEFLYLNYSDCEVFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S4T3</accession>
    <name>A0A8S1S4T3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0030389</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S4T3"/>
    <dbReference type="OrthoDB" id="297175at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8134602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="313" mass="36370" checksum="BA7DB4FD30B78D79" modified="2022-10-12" version="1">MNTQDEILLLKKCICEFLNKLLPTIKDPLILTRQRLSQFKTVLLDELKTIQDVYFYVQTLQFQEGLILRTDPKIEHYRNPSGTNLDILNLNSNPTISEKRVIPNSLGLNRGIDSLQTNVGNYTNLKIENTTTTCQQNPNVEDQLDKEIPKLGKDIFFKRPSTRKLSIKTKDTKAQENNYKPIDEIQKLNVYGQRVDTFSVLNNYSKKTSFFDKETLLNEIKESEKIIRKHTTTLNAVYSRSFEILTGDKEQNSEQLNSIQQSLRAQQDIRELQNAQRNATLKGYSIYAKKDKDIAKQRIDEIAQINDKKKLYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S4U2</accession>
    <name>A0A8S1S4U2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0030219</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S4U2"/>
    <dbReference type="OrthoDB" id="292391at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39867">
        <property type="entry name" value="HELICASE ATP-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39867:SF1">
        <property type="entry name" value="HELICASE ATP-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="217"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8134270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="893" mass="106611" checksum="78727F8D0D78E1DA" modified="2022-10-12" version="1">MKKSYSQNNSGNFDFPEVNKPRFEPQLHKISASTQQKVVIQKLTNSLNNNKIVTQSTEQPQTIRSQIQKPFINSDRFMFDLDASMVYIHREKIMQFEEVEQQRIVQLLKDVQSKVPQVNDKVNHFIALAGQFENEKVNPLVNQFIKVVEQLLQIFDEKTKEIKEYQFALKQSREQVQNNSRLIEDQSQMIKYMESQNQNAKNVTRTFQRSNELAVINVQLEKKVELLNQNLQFLMNQNKDSLATQLQQKIDQLNKTIEEQQNKLYDDNNKQIQLQQQIQTLKSTEFKHQIQLRKLEDKIQEYESKLKELTFQVSYSKEQKDQYRELYYMTYEDLQMNKLNTQTERKQMQKSFEKIRMLQDRIDEMTVKRSKEIDAPFQSRVEGDVIQLQAITKILDEDHVFQRYMMSSFLKNVQEYDSKLVDAYVDVKRLVIGRDEEAELQTLRYNYPPFTLFLEKRIVDQETQYLQIRPISNNFMGLLRAILDGMYIEFQKNTYISFQSYIISWLSTFTIENHQVIIFPEDTKRVKIEEQLHTFYLDLMVPRLDKVWEVVQFRHFLNDIYSLEELYFYLHVRFLLRRGPWIEQFDAIYGVVHYMKVQQAESIILRFLEQFDKINQTLVMRAVNERIVEGKKDRKLIQTGFVLQLLLEIFRIDRINRYKILSFAFPAQPINFKQFHKFVRGNFSKCTEGEAAELWREVYMISNGIPNLNSFFTAAQSLFIKSLILPAIYTVPLLDSNRKLAFDELNFTTMKIQEQMKELTDSDFDVLKTLIPTIYQEEMIRITKQLMPKNQIQFVNPLEYIFKFMKLILTLKYNTILADGLEEDGVNRKGFITETFTQFITQEINELNKITYKKQSFMSDQEIRISRLQKFAKKKVKKIYVIMSSVLQSRIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S6D4</accession>
    <name>A0A8S1S6D4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0040381</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S6D4"/>
    <dbReference type="OrthoDB" id="331948at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45911">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45911:SF4">
        <property type="entry name" value="MULTIPLE C2 AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="4">
        <location>
            <begin position="94"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="75"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8135718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="479" mass="55956" checksum="4A9C7A7EC29D9B2D" modified="2022-10-12" version="1">MLNLQTILRGFEENGLKQESLYKADEILAKLDVMGRKEFDRDIAEQLFQQCKPTQLNLKVAFKLADIGQTILDAIQLQTEKAKNAEQQLKQIQQNKAICENQLAETSVYSNTRYLFLTLLYVKNIPLQLKYTNCHIQLTLGVINQIAKPESSYDKVNPQFNQDFEFSVPPLISTLQIQFYIRTENTLPNLWGQTQLKIQQLDDQVIKKVELQLQDPVGRELDSSIQLEAQVVLNKHQYLQAQLQKFEQKIITLDNDLIEYKNNLEIIEKPFKAKQLLKEQNNKQLSSNIFNQQLHTSQNQQDQDQYINEIDQQQHQLNSPQENKEQILSILQSDKQLEIQEETPEAPEILQHGVIIFTIYGLITLFVCSAKPSFLDVLVCHGLMFTILMDKFEPFHIKLVGAGLLASILYDILWMKQYHLWWYSDDQNNPEWGLNAVNLLRFVLIFTYIESIDPTKRYVFTIWKIKYKVGKSRESVSWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SFV9</accession>
    <name>A0A8S1SFV9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0080007</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SFV9"/>
    <dbReference type="OrthoDB" id="303291at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="12"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="444"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="455"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8139255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="494" mass="57054" checksum="7E731AE82444D04C" modified="2022-10-12" version="1">MLNKSQNSMQICQLCKMTSQNPIQLICSHSLCLKCGLKIQIQENTSQIQNKFRIQCPKCNKMTHTYDIKNLLLESNELYLITDSSEMEKEVLNESQFSLQKNQQEAIFGRRESNAYQIQEVFSKNSQMLNVQAINNNAQTAFNHNHSNKKKAIITNEKTSPTKQFQKPAQQNHQKKQSYQMPHPEIALECKAKTQANTSNVQQQKLNDFLKPNSNTPVATPTTLSSQLLFQQTDNKKQIAQVQAVQQYKQKQKTQNTNIYVQEHLQNAINQNQSDIVFPQQIKKNNNQNNLESVYHQQVQHQISIFDKYNENNKQMLFDDKQKCTIEYNKEKENQFLKEISKDCQNSSNQTSDNSRHKRSSTGLIQEVEENASVQIIQSYFQMVQKNVQKLERDVINAANGKQIDPQIVYSKLKVLTSRIGDNDGELVQSIKVMQKLQQEILGLSTPSQHRKTSSHQNTSHRYTNSSNYGKNIEKSDQSIGAMPQELKIYKKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SGA6</accession>
    <name>A0A8S1SGA6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pantothenate kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0080082</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SGA6"/>
    <dbReference type="OrthoDB" id="498611at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004594">
        <property type="term" value="F:pantothenate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd24086">
        <property type="entry name" value="ASKHA_NBD_PanK-II_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004567">
        <property type="entry name" value="Type_II_PanK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12280:SF20">
        <property type="entry name" value="4'-PHOSPHOPANTETHEINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12280">
        <property type="entry name" value="PANTOTHENATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03630">
        <property type="entry name" value="Fumble"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8139405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="342" mass="38938" checksum="3081B8CCCFC9C0A7" modified="2022-10-12" version="1">MQNNPSFSQGLIPIVCLDIGGTLTKVTFAAKTNFILNCQTKQELKIKNEDQDYEIHFLSHPNSLEVLIQQLEQIGFIIDGKSNIQTFYITGGGSFKYYDQISNYGNIVRINEFDALKFGFKLLDSIKYKNTYFTFNNGIEYLQLTASIYPFILVNIGSGVSILKFDNEDKFARISGTCLGGGMFLGLSHLFTGINDFDELLKMTKQGSNAATDLLMDEVHLGFQSPTRNKNEKHVAVSMGKLKQNSNINQSDLVKSLLYMTTYNISQIAFLHSKLHNIERVFFSGHFIRNHEETLQCINEAFSYFSKLDQQNRHPYFIKHDGFIGSLGSFWNGIQTYKQNNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SKY5</accession>
    <name>A0A8S1SKY5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0090140</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SKY5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8140568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="386" mass="45510" checksum="03D1DD6DDED76229" modified="2022-10-12" version="1">MPIFKGLFDVQQLQIITEDSDNQYYHLIDEQFIQETIINYLEVLANTQNEKFNMPSIQAILSFFFQVKNFATQQNLFKVLSQIFLCYSSPIGVELVQEQIGSTYELQIQNYVQQRNQMKERLNHLFLIKQLSIEESNRINSHINLYNFAIGIYEGINKGITNKTILTWSISAAEKALIGYRIVSKQFQRQIHYQIADLHLILTDCFIQLEQLSTIIFHLEAAEDELMSWFKQNKHPLKAHFLMKQGALRPWIQEQFILYISWMNELPRLNRLNIFTIIRGLLLQNRKLLQIFEQNNQDLINLEINLLFYLKGLDETQQVYVDIFQLCKNNDPELILKQVTTLSAFDFNGVNILMDAQAIFQTFNQNNCFTQKLQIMIDQMISQQII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SP69</accession>
    <name>A0A8S1SP69_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0100267</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SP69"/>
    <dbReference type="OrthoDB" id="283202at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051291">
        <property type="entry name" value="CIMAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580:SF28">
        <property type="entry name" value="BOREALIN N-TERMINAL DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580">
        <property type="entry name" value="SHIPPO-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8141710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="225" mass="25353" checksum="3790D1C91109CB50" modified="2022-10-12" version="1">MINNENNSQHLKSKPLFSVMKLINNNTFRGQFSEPNQLSSSILQQTNPQLSFPRAERFQSHHTPQLPNPKYLCLTDTKTKRATSFGFGNKQLRPLDLDKRDQLNPAPGTYDIKQTSCRSCSFGMKLQSIRGFDVPGPGSYDLKLLNKTKVFSIYGKINSDIKLPKTASPTTYLPTDKIIQNNRFKQITFGIGDRPLPGLKNDSPGPGTYALKSIFEIKSKKKTQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SSQ0</accession>
    <name>A0A8S1SSQ0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Adenosine deaminase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0130306</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N(6)-methyl-AMP + H2O + H(+) = IMP + methylamine</text>
            <dbReference type="Rhea" id="RHEA:16001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:59338"/>
            <dbReference type="ChEBI" id="CHEBI:144842"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:16002"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; purine nucleoside salvage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SSQ0"/>
    <dbReference type="OrthoDB" id="272271at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004000">
        <property type="term" value="F:adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006154">
        <property type="term" value="P:adenosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046103">
        <property type="term" value="P:inosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001365">
        <property type="entry name" value="A_deaminase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006330">
        <property type="entry name" value="Ado/ade_deaminase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11409">
        <property type="entry name" value="ADENOSINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11409:SF42">
        <property type="entry name" value="ADENOSINE DEAMINASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00962">
        <property type="entry name" value="A_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="4" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Adenosine deaminase" evidence="9">
        <location>
            <begin position="3"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8144601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="296" mass="34163" checksum="FE4A56C461AF515B" modified="2022-10-12" version="1">MDKIELHAHFSGCIRFTTLQELTIRQFGQNQEINFQKCTTLEQCFILFAKINSLNLMLDDVRRIADEIFEDFYSDGVTYLEIRATPKKGQDFDQLQYLKAISDAINKAKFEIKLIVAIDRAKGIDEAEKTLNLVKKNKIQHLVGVDLCGHPGIGHFSQYKSILQKFRDLGYKITVHTGELKQQIEENNDVIDFQPDRIGHFIYFTEEQLNKIKNLNILIEVCFSSNLFTTNMHPDCHPVKQFISQGIPIAICTDDTLCFNTTVTKEIELIKTTFGYSNEFISSILKQSLNYKFKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SSS9</accession>
    <name>A0A8S1SSS9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0120116</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SSS9"/>
    <dbReference type="OrthoDB" id="304597at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="313"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8143253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="394" mass="45546" checksum="4174B44125745CDB" modified="2022-10-12" version="1">MSSYPQVGLFDNQLTNLDSLSNDVHLIQQTLHGWTSIFSQLMEISIETHKQNQSNTALLQHLMAKMHRRSKHSQPKDQIKLSPIRPIKTEPDDILKLENFSKIFNNPKKLQGTPFDSAYESLNKELEEPTRDEQYLSVPKSIQLPTEQNKKKKSKQIDLHPQSTKNQYENKQFAFNKPTHLQDVKSNKENNLNQLSINRPESKRNLDCPQLSTVLEEKQSALITPNENNFKYSIAQSYRQYDQCSTSKASQNSQRPPNYLQQQQLQSENNRSSSKSCYGANSNLQQQVLTNNINIMNSPQNFQLIQQQTTQNQSQNNLISSSTKNVKQQNSQNNNVSNKQPIEIVNQIPSQRAKSYSEQNGKKQLSKRCLDIDLLDKQYETSQELIWKVQKNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SXP6</accession>
    <name>A0A8S1SXP6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0130028</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SXP6"/>
    <dbReference type="OrthoDB" id="6581954at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10324">
        <property type="entry name" value="SLC6sbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF240">
        <property type="entry name" value="BLOATED TUBULES, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0769">Symport</keyword>
    <keyword evidence="1 2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="96"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="205"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="248"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="319"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="386"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="414"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="453"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="485"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8144099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="533" mass="60331" checksum="56D41708F01364FA" modified="2022-10-12" version="1">MQPSSQSLEGYQEIRMKFSSSWTFMLTSLGFAAGFGSVWRFPYLVFKNGGGTFLIPYFILVFTLAIPLFFLEVGLGQCKGIGMAHLLDLEKPKLRGFGYVGIVICAYISTYYNLIMAYSLRYLWESFKYPIPWLESIVEQNKPFSRDYFYDSIVQMSTSITDLNHVIWSLFIAYIVSLIIVYFCIKEGVETSGKIAIVTATSPYILLVILLIRGLMLEGSIDGIYYLFKPDFVKLFNPSVWVDAANQVIFQMSVGQAILCLYGSYRKKEDNLPNFSFAIPILTALCGMLAGLVVFSYIGHVSFKYNVSIQQLPLSGPDLAFVLYPAILAQMPMPNLWCILFFLVLLLLGIDTQFGFVDGIAGTIEDIYLGEVIVFGNKLTVQQVRLCVCSILGMIGLIYCTDIGFYLLSFVDTFGTNLSFMLGVLFEVFYFGSKERFEQFKIDLEKYGNHVPWLVEFSLTKLCHYTVVVLLIISIISQIKSSLDYSIYVVLIGWIISFSPFIAAIKIYIENRNMIDISKTGLDIELLTKQVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SZB7</accession>
    <name>A0A8S1SZB7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0130001</name>
        <name evidence="2" type="ORF">PPENT_87.1.T0130002</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SZB7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8144064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8144066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="99" mass="11883" checksum="E2E74889DCD20D4E" modified="2022-10-12" version="1">MECDNLINGLNTYIDNLNQSFSLSEMVIRYKYSLQNERLVHLISFQINYYLHSTIKFIGYKQSITKIISEQASKLTYTFNNVYQYLLLSQFHYYQIDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T393</accession>
    <name>A0A8S1T393_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial</fullName>
            <ecNumber evidence="9">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Succinyl-CoA synthetase beta chain</fullName>
            <shortName evidence="9">SCS-beta</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0150224</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>succinate + ATP + CoA = succinyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9 10">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the succinate/malate CoA ligase beta subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="9"/>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T393"/>
    <dbReference type="OrthoDB" id="1552at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042709">
        <property type="term" value="C:succinate-CoA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006104">
        <property type="term" value="P:succinyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000002">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000001">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00558">
        <property type="entry name" value="Succ_CoA_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013650">
        <property type="entry name" value="ATP-grasp_succ-CoA_synth-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017866">
        <property type="entry name" value="Succ-CoA_synthase_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005809">
        <property type="entry name" value="Succ_CoA_ligase-like_bsu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001913">
        <property type="entry name" value="PRK00696.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01016">
        <property type="entry name" value="sucCoAbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815:SF1">
        <property type="entry name" value="SUCCINATE--COA LIGASE [ADP-FORMING] SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815">
        <property type="entry name" value="SUCCINYL-COA SYNTHETASE BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08442">
        <property type="entry name" value="ATP-grasp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001554">
        <property type="entry name" value="SucCS_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01217">
        <property type="entry name" value="SUCCINYL_COA_LIG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2 9" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="ATP-grasp fold succinyl-CoA synthetase-type" evidence="12">
        <location>
            <begin position="14"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-citrate synthase/succinyl-CoA ligase C-terminal" evidence="11">
        <location>
            <begin position="289"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="72"/>
            <end position="74"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit alpha</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8146198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="429" mass="48096" checksum="D5B38A2F7F531456" modified="2022-10-12" version="1">MFRFNRIPKYFLSLHEYQTAEFFKSFSLPIPPGRICKTPDEAHKAALKIIQEGSDRNSFTDVVVKAQCHTGGRGKGYFKENGFNSGIHIASTPDDVREYASKMLGNTLITKQTGNLGKKCEMVYIVERNFLRKELYLSILLDRNTGGLGIVASEKGGIHIEECDPNYIKKFSIEMPNNVDDIDFSIYEQVSKVYKLNPIQHNQMKDILKKMFDIFLQTDATLLEINPLGIDLQGNLIICDQKLNIDDNSQFRQHAIFHMEDVRQKDWKEVEAQKHGLNYIALDGNIGCMVNGAGLAMATMDLIKQYDGSPANFLDVGGKATDQEIVSALKIMDKDPNVEAILVNIFAGIARCDQIVLGLIKGLTVLGMKKPMVLRMKGTKLDEAKKLIEESGFNMMFTEDLDEAAKKAVRMAAILRLAKEANINVNLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T5M4</accession>
    <name>A0A8S1T5M4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA-dihydrouridine synthase</fullName>
            <ecNumber evidence="9">1.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0150130</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the dus family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.-" evidence="9"/>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T5M4"/>
    <dbReference type="OrthoDB" id="10262250at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017150">
        <property type="term" value="F:tRNA dihydrouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02041">
        <property type="entry name" value="DusA_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001269">
        <property type="entry name" value="DUS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004653">
        <property type="entry name" value="DusA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008774">
        <property type="entry name" value="PRK11815.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42907">
        <property type="entry name" value="FMN-LINKED OXIDOREDUCTASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42907:SF1">
        <property type="entry name" value="FMN-LINKED OXIDOREDUCTASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006621">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4 9" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="8 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 9" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="2" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="10">
        <location>
            <begin position="11"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR006621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8146012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="346" mass="40166" checksum="2C5CD298A7AA4EAE" modified="2022-10-12" version="1">MEQAFRHKISIAPMIDITYEHFRVFFRLLTKNCVLYTEMIHENAINLSRVLKPFPLVLDYSQVEHPVVCQLGGNDPEKLAKAAQIAELAGFDEINLNVGCPSERVQDGAFGACLMKEPYLVAKCMKTMKEAVKIPCTVKCRLGVDNLDTYEFVRNFIEIVNKEGQVNHFIMHARKAFLKGLNPHENRTVPPLKYDWVYELKKEFPQINFTLNGGIKCTEHIDQVMPNVDGVMIGRTAHENPWIFSDFDRRYYGGENQNYNRKEILILYAEFVEEKRATGCKVSTQMCVKPIINLFNGEMNNKKYRQFLSNTDNHKLGFRGMINQVIEHMQEWNQEALLVRPNDYKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T9I2</accession>
    <name>A0A8S1T9I2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0180410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8149090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T9I2"/>
    <dbReference type="OrthoDB" id="10251741at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47822">
        <property type="entry name" value="CARBOHYDRATE BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47822:SF2">
        <property type="entry name" value="F-BOX AND WD-40 DOMAIN PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="128"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="177"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8149090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="349" mass="39744" checksum="F4A8C7BA721CB4D9" modified="2022-10-12" version="1">MQSTQNQEDQQQNNQSLEPNKYEFKFGVHKNQVGLKLQQQLNVNGGDVFSLRYDQDDQYLAVGMSEGLIHLYNQNKLAHTLEQFQTPVTALRWRPNYGPKAKGILVSANAEGHIIHWHAHSGKQLHKIVEENNSVLCLDFNFDGSLFATGGKDFCVRIYDDDIKSVQHQFQSADWGQRGHANRVFVVKFMPDQPNILISGGWDANVLIWDIREKQCVGQFYGPSLSGDSLDFSVKRQLILTGSHRTENQIQLWDWRTRKLYQEIFWTGSQSDDNRSYYIYATQFNKANEDYIYAGSSGLHEIKLFSLKDGQAKASILGLPKGILSIDQMNTTDSIIFSGIEGIVNIFKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TDT8</accession>
    <name>A0A8S1TDT8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0200064</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TDT8"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043169">
        <property type="term" value="F:cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000051">
        <property type="entry name" value="Protein phosphatase 2C-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="4">
        <location>
            <begin position="159"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8150048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="444" mass="50411" checksum="26EB44881D758FA2" modified="2022-10-12" version="1">MNKANRTTSLRQHKLTFLKDNNNSIQLQTRSQSFKQNISISPDKKSQISPTKQNSVFLNGQLLTRPRSKYNQQSNLSTSKLQDSQKMFLIQQAQQQQAQQQRAEQSPVLHRKQQSDKVLKYDLPKISPIKSRQPIKQLQPDMNQLDLFKREQIKQNIIKNAFTKSQAGKNEDNLTKTNQDSFISLQQFKDNMSLFGVCDGHGQDGHKCSQFIRDNLPKNINSLLSQNPSSITESISKSFIRTNNQLCNFEEIITTFSGSTTVISLIVDDTIYTANVGDSRSIICRLQSNGVKTAITLSNDHKPDLPQERRRIEQNGGRVEPYIDFDGSSLGPSRVWLKSEDIPGLAMSRSFGDKVAASCGVICEPEILTHKIQEGDLFMVLASDGVWEFLTNEQVIDMIYPYYIQDEGNAACVRIVKESIKLWKLNDTVIDDITIVIVFFNKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TFH7</accession>
    <name>A0A8S1TFH7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0200228</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TFH7"/>
    <dbReference type="OrthoDB" id="292549at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8150374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="291" mass="33219" checksum="3AD1991309F1BE84" modified="2022-10-12" version="1">MENREQDVPAKENPIAEVRLEQKLNEIEEVGEKGINEIEKYYKITGSPINNAIIVQIFICALASGSTALVNSLGPVRWLISLIPVFNLLNFIIYAVFLAFLQFGSPFFKKKPWNFLFFSIHFIGKFSFMVFYGVNYPLSKFEVFYFLCVFGYLYVLALVRNRDAGLNGVDFTIKGNFFQLAGLGIFLGFFLALLTQAGFWPMIWQPVLGVLYFLYLLLEVQRFDGSLEYLREGKNALFLGAAQIDADLLWFCPLALFHIKSKGDNIVNKAQEQFEGAKEETKKLINSEQNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TFS8</accession>
    <name>A0A8S1TFS8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">60S ribosomal protein L23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0090121</name>
        <name evidence="6" type="ORF">PPENT_87.1.T0160292</name>
        <name evidence="7" type="ORF">PPENT_87.1.T0200299</name>
        <name evidence="8" type="ORF">PPENT_87.1.T0220294</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8152131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TFS8"/>
    <dbReference type="OrthoDB" id="294753at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00337">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.150.20:FF:000008">
        <property type="entry name" value="60S ribosomal protein L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_uL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_uL14_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761">
        <property type="entry name" value="50S/60S RIBOSOMAL PROTEIN L14/L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761:SF8">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8140530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8147268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8150514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8152131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="140" mass="14962" checksum="EA72B22757D0EABA" modified="2022-10-12" version="1">MSKRGRGGQVGIKLRITLACNVGAVLNCADNSGAKNIYVISTFGIKGHLSRLPSASIGDMVLCSVKQGKPALRKKVMQAVVVRQRKPYRRREGYYIYFEDNAGVIINPKGEMKGSAITGPVGKEAADLWPKIASAAGSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TH52</accession>
    <name>A0A8S1TH52_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0220009</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8151600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TH52"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8151600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="119" mass="14166" checksum="7CCCE946FCBE3734" modified="2022-10-12" version="1">MEIQGIQIKQNNIFKHLQEKYQKLEQTQIIQKKCQHLANKLNEIKLQKMNIIKKTLFVYIQYLNIFIQNSIKRYEGAIKVLISNIMITYAAIDSGAMNVEIDKYSKLWMINQKAKIYQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TN00</accession>
    <name>A0A8S1TN00_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0240019</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TN00"/>
    <dbReference type="OrthoDB" id="301355at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8153077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="130" mass="15315" checksum="FE80E4EBA0883B08" modified="2022-10-12" version="1">MKRKSTFFKYGKHQSPDELIKHLNQCSLNPKFIENKSTADVTKQNTVVLNKIELRKSKSRDCDQSQTINRSRLQREYLRFLLTTDGPSSEFYQLEKKKLQIKLQDIYETAQPNICLSKILGQYQELVNPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TPA0</accession>
    <name>A0A8S1TPA0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Insulin-like growth factor binding protein, N-terminal</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0250209</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8154120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TPA0"/>
    <dbReference type="OrthoDB" id="317300at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035868608" description="Insulin-like growth factor binding protein, N-terminal" evidence="1">
        <location>
            <begin position="17"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8154120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="501" mass="60295" checksum="0DEE92016FED5BCF" modified="2022-10-12" version="1">MFLIFHLFYICISSFTYINDQNRYSVDTDWSNPTYSQGVVSKLCPLGAKYYMYETINTDIYYFLSSGILLEGNLFYLHYVELSNSKQKIIHHVYYKQENQWLSNEFIFNTQDYEGRWFQQYVNYDIEQKLLNFDMRWGNYEKALNQYSFQQFEYKSFNFVIGGSFNVEHPITQTMLYLKTFPGQLRAAISVYQRDMDDAWDIYTETQCSETITLLLGQLEMREFNQHQIYMIENQNLQYYKVTFWAKISKEYDPEIDQKIHILRIVANRFTTDFQATGSNSFILYYVYYSEQKQWMMIIRYYSYALPIIVQFTDNNDPFLKEDVIFIQNYFLLSTWHYIQISYDPYNLNFYLINHKQDEQIEKTYQNVYQFSNIYYRLFFGGSSEELSSLGRSFGLISYYDCNNKNIQCHYSCSTCYGPSQNECLSCPESTNRIYNEVDQTCKCKVWYSETSDFSCQFVTQNEVIVDLVDEQEDQDLEYCKFGEFIRQQVNTIRSYVQNVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TQG1</accession>
    <name>A0A8S1TQG1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0250184</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8154094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TQG1"/>
    <dbReference type="OrthoDB" id="298359at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="134"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8154094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="485" mass="57409" checksum="7B52CBD3116FDDA9" modified="2022-10-12" version="1">MKSSLYFVSSERQPFWNKNLKSLVNYDFHKLSEDQQTVVLFKQGQLFKRTTARRYKIYNQHLFYFREVIIYQRMVRSVAFQFQKINKESLLVITLSHDQSQLQLFTGNRIEEVENFINELKLNCIQEQMLINFYKINEKIGEGITCEVFLGSNQQNQKFAIKKVAKAPFGHKMRDKQAKSLAEEIFIMRSLDYPNIPKLYEVFESDNYIELIMTYYEGGHLYEKLQNFSLSHKQQLVKDIVETMNYVHSQQIMHRDLKPQNIMYKDKDPKSTDIAIIDFGFATNLKYQEHVLYNCGTHGYAAPEVQEYKEKQKMYTTQCDVYSLGIIIYEIFFGVHPFRNSNIGTLTFNDKIKVPQSLKNLLIQMTRIQPKQRFTLKECLDQDFFRENFDSQGLDGLSKYSLVDSIYYKSLNISRKNSIEQSIKQEKISKFESAKNKTVNSTAAESKNILLKSLNPYDQQFQDDLQFNEYHNCPNDFNNFLQKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TQZ9</accession>
    <name>A0A8S1TQZ9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0250377</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8154448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TQZ9"/>
    <dbReference type="OrthoDB" id="293282at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8154448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="192" mass="22173" checksum="9E69E71C5A057F5D" modified="2022-10-12" version="1">MDKELLIDCLYNKELIKQHLSQTRKAQNDILTKKPSSQFYHVQSSKYGVRQPVQIKNPLLIKSLFSSRKFSKEKDISLPICAFKILPTQTLASFQTDRIHSRCPAFKKLKLKLGLKQAKSVPDEQFRIETAPVQCRSQTSTKQSINTLLKSQPSYEKKTKKSEPAFTFNKNDWGIGGWNIIEENYDAKMFID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TRR5</accession>
    <name>A0A8S1TRR5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0240333</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TRR5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8153649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="108" mass="12612" checksum="661E64ACDC4171A2" modified="2022-10-12" version="1">MKMGDRLSQAIDLEWILKSLLMVNIKMVRKLGNFIGKTGKYLNNVRQKYQILSLNLKVEVDGMMMEEMKSKLINGMSLVKSLKMNLVTYKGEYRDGKKFCNWDVCRII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TZS7</accession>
    <name>A0A8S1TZS7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0280163</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8156166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TZS7"/>
    <dbReference type="OrthoDB" id="287627at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8156166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="162" mass="19123" checksum="D3FEA65F040F508E" modified="2022-10-12" version="1">MKKCSLALQTSLLFDEQEDFQAHDGDGLGPWFKMKEKKQLAAPLQPIRIPQIRIKSQQQQKRVLQIKTERYNTERAMHVNEDPIRINNLKQNQEYTDSSSPVEQIIKKHFSNKQSVFFPKSQQSSFVQKSDRSNRQIRVKKLKICNSDIPLINQYNYFSRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U1I8</accession>
    <name>A0A8S1U1I8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutamate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0310251</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U1I8"/>
    <dbReference type="OrthoDB" id="6718861at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01076">
        <property type="entry name" value="NAD_bind_1_Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000100">
        <property type="entry name" value="Glutamate dehydrogenase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014362">
        <property type="entry name" value="Glu_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033922">
        <property type="entry name" value="NAD_bind_Glu_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606:SF13">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000185">
        <property type="entry name" value="Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal" evidence="6">
        <location>
            <begin position="207"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8158264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="492" mass="54355" checksum="31DFC9E7580ADD71" modified="2022-10-12" version="1">MYIQQIARSIINRSCFSTATINEGTFLDQVMAYFNKAAGHTKISQDKLDFYKSTDIVIKFNLPLVRDDGTYVCVPAYRAQHKTYRLPTKGGTRLSPHINIEEVEALSFLMTLKNSILDLPYGGAKGGIGINPRKFSKREIETLMRRYTLELAKKNFIGAAIDVPGPDLGTGEQEMSWMKDAYTKFKGHQDINSVGCVTGKAINQGGISGRQESTGMGIFFATREILNDVKYCQSVGIEPSLKGKSIIIQGYGNVGSFCAKYMYDYGATIIGVAEHDGSIYNPNGINPYELAAHKTKTGGVKGFVNASKYWEDESAIYQQCDIFIPAAFEKTVNAGNADNFNCKIIAEGANGPTTMAAEDKLLSKGVIFLPDILLNAGGVTVSYLEWLKNLKHINPGRMTRRWEEQAKHRILEVIKMSTGLNINIKDSKLAKKMLEGPSETDLVHTALEQSMIEAVNNIMATSKEYKVNLRLAAYISAINKLNEHFEMAGIEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U4Q2</accession>
    <name>A0A8S1U4Q2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0340013</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8159830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U4Q2"/>
    <dbReference type="OrthoDB" id="307588at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="217"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="312"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8159830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="349" mass="41419" checksum="622FE8329381C318" modified="2022-10-12" version="1">MFFDSIFLFLPQLQFEYLYPILIKDKYTQYLYLFFIYKNQTIHINISLNFKQKQIYKMSKVNDTQTTNVPMVVTKQEINEQGIFNETSKALGWYQYFPESPIVASQEYSSHDQQMLFIVKLYIFLAGLFILQYLMVIMFYYSFEFRWSLIFLGYPTPLYWVIFSSTILLGLMAYLIKLFRRPPFNLIVYFLYSFGVGCVLAAPYADQLWWNENSLIIILYQFCMTVCTFGTIIAYKFKDSQFINASYILLIALILDVFIIFIFIMIYTELAWLIVISVLIHLIYVCLLLFETRLIQRGKFNLNLDEYISGALFIYLEVTLFVIFVGILFIIFMVVAFILLIIAFIAHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U7F9</accession>
    <name>A0A8S1U7F9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0330052</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U7F9"/>
    <dbReference type="OrthoDB" id="312635at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8159279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="433" mass="52114" checksum="ED0B5AE1B3B1D797" modified="2022-10-12" version="1">MIKSQQEDNCKIQIETERKDQKICGIVNFTFKFQADYQLEYKFEDCLIKNHYLKIDDFEDLVFPEDLNINYCYSNEQGEQIQLDKQDYEIDDISDHDNSYEYNNEGISSFYVQYMVISPNHQFIFLSVIIKILNQQESYRLMVIDIKNQKIKKFCYRNYLPGQRPQFSKNGNVAIMNISKNDKGSRYIFFDLQNDLQKVTIFKFRRQQLLRIEYDDISQCFFYITSSGKLIKQPINFEDYTIDKSKCWKFNICLILNEQLYRYVRFYLLYDSIGLLLDENGIFIVIRIGKNCKVIRNYICKSNPVYIFNKAFVVASDYYQNKLLVIDALKGKLIRKIDFSQRTDKYQYYYPPKRSYYVSYLQYYPQEENSDFEDSSMPQLEKGSKWMIFDVIRGIEKEVNKTILTQENIEKQIFTHNFVAQKYQNKISFQLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U8S4</accession>
    <name>A0A8S1U8S4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0360081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U8S4"/>
    <dbReference type="OrthoDB" id="295859at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035712884" evidence="1">
        <location>
            <begin position="16"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8161260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="342" mass="37990" checksum="70E6CBFA698ADDBD" modified="2022-10-12" version="1">MKTFVVICLVAAVFALDTNKFAVLLQTGTRGNDAVESVYNLLRDLKTENVNVQAAADKKNNTDEEIFSQVIGDLTNVASLNKQQWESLGVVRTDVEAQVRDGYQWLAWAEARLAEIVRRNNQLQDQRCWANGLFVRSLADHADAIGVVQLLQQDVAGFLTNNAGVELVEKAQTIADKLSAYSHLFQQDAIQKFQSLAEVKREGTTGEQVLQILQDLQVELESTLATLQEQEIHAAFALAKYVSDTNAEVAWLTSEQERRTGLVEKLETQLPAVLAQQAKALKLWKDSLNAVAGATADLEEKREFYASETARRAEENAIIDVVIQLFKDQVRSLASQTSLGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UB12</accession>
    <name>A0A8S1UB12_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0350219</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UB12"/>
    <dbReference type="OrthoDB" id="300613at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8160882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="136" mass="16223" checksum="F089B9B09D48B335" modified="2022-10-12" version="1">MNDAKIDFLKWLQKERNDQFTQLFSEDVLLNIESEKEISKLQKISLNNYQKLIYSGSNIQLNKVIKSNVQEINQKNKFQQTKINDLIDNIQLGENFLDQNERIDSRNYQQPQISRQPKQEFLKEEIPLRKLIAILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UEF1</accession>
    <name>A0A8S1UEF1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0390130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UEF1"/>
    <dbReference type="OrthoDB" id="286314at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="82"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8163258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="197" mass="23861" checksum="8DAE102179A9E38C" modified="2022-10-12" version="1">MNTETQSEKQDQRRSKRERYVGEALNAVQKWRDLFENGYYDEVGNYLKPTLKEAADLVGLPKRSLEQYYSVFRKIPQSIDIMRFLDKKMGNLNQFIKELKNESAITQVKEKEEEQEQNSWDNMIIEADDQLAVQREIDDEPELQQILQQQQYYENEDDYIKYLQLEEDQKYCSEYYFYDENDDYFMRDDFNNPALPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UHZ3</accession>
    <name>A0A8S1UHZ3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RING-CH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0400164</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8163984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UHZ3"/>
    <dbReference type="OrthoDB" id="264354at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16495">
        <property type="entry name" value="RING_CH-C4HC3_MARCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46065">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCH 2/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46065:SF3">
        <property type="entry name" value="FI20425P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="206"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="241"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="11">
        <location>
            <begin position="116"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8163984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="316" mass="36984" checksum="316560AE51C19317" modified="2022-10-12" version="1">MIVTINNYSGYQGIKKKEDATSTQITERYAQESQNNTILTQNLGHYQSRQQSDANIEQFNLYPNDTHMYNTPKKQQNQNTEFIEQSNEILKNSKINITTENVNIITRKLSSSTIVVKKMEEQECKYCRQVDSLYNFIRPCLCKGSMQHVHQQCVQKDIESNFMDENQRKFIKPIRCEICQFVFKIKIYRETNVLQSFKDPNKHEKLLFLSFVLIILTLITIGLVILSILIKEELRMHIDLLMGIVIAFLIVLGLMIAFSLNFIDLITNYYWFVLDRQIANKVDNGIQVDYQSLNLILLSGSLKYQWKKSQVLPLNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UJA9</accession>
    <name>A0A8S1UJA9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0400091</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8163842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UJA9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8163842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="111" mass="13735" checksum="2EC0CD7152A9D59A" modified="2022-10-12" version="1">MLETKELYYQYQQKLIIQMIQLYLVPQKKQNDFFEVKTESLEQVPQKNQNHMTKIQRQKMRQEQEIHLDTLYLKHHSKQVLSKQRKLFYQGIYPEDLDDYTELNKSISNYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UM27</accession>
    <name>A0A8S1UM27_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Brl1/Brr6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0430258</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8166050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UM27"/>
    <dbReference type="OrthoDB" id="298076at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006998">
        <property type="term" value="P:nuclear envelope organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040202">
        <property type="entry name" value="Brl1/Brr6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018767">
        <property type="entry name" value="Brl1/Brr6_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28136:SF1">
        <property type="entry name" value="NUCLEUS EXPORT PROTEIN BRL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28136">
        <property type="entry name" value="NUCLEUS EXPORT PROTEIN BRR6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10104">
        <property type="entry name" value="Brr6_like_C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01042">
        <property type="entry name" value="Brr6_like_C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Brl1/Brr6" evidence="2">
        <location>
            <begin position="61"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8166050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="186" mass="21733" checksum="3AD0271A37ACCBA9" modified="2022-10-12" version="1">MSSENQITRFSEQESLMDLKSEFMRRINKNHMKMELSERKHDDGSLEKSIKLTPMTIGDFIGIWAIAILKSLFLICFLLMAYETYKDIQAHIDISKTQVLIEMKNCAAEYEQNQCSENYLIESMSLRCKELEICTNQNIEMKVRTQQLWLQVLGNSFEKMFKELSLKSCIMISFISIATSIIFIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UQD4</accession>
    <name>A0A8S1UQD4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0460117</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8166885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UQD4"/>
    <dbReference type="OrthoDB" id="290122at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035723020" evidence="1">
        <location>
            <begin position="16"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8166885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="269" mass="30697" checksum="64C4358157DBFF5D" modified="2022-10-12" version="1">MKLIILLTFILIVNSADIKACLCSNLYTEFDCSNAVSFQAASQDLFDVKVCIWLEGQCIDIIKDFDFPCNVLDEDTCNNIPSCAWQNLQCQKFTKCSDYTYEKSILSKSCDDINCLDNGTQCAFDLIGRSEQYCEDIAFEEDCSRIYLLYKKTLCNWNGSACVGQKLSSCSSLTEKQCLQANAQCAWKNNSCVKRTCEDGQYSFYQSEGVKLCSSYLDIETDQIMYCEINEDGQCGTSNPEDLDRRNCYSQSFQQYYWNPDNEECQMCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UUN5</accession>
    <name>A0A8S1UUN5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0480126</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000048">
        <property type="protein sequence ID" value="CAD8167977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UUN5"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000334">
        <property type="entry name" value="Heat shock factor protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF206">
        <property type="entry name" value="HSF-TYPE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00434">
        <property type="entry name" value="HSF_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="3">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8167977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="246" mass="29143" checksum="0C34E6D432770CD6" modified="2022-10-12" version="1">MNTRTPIPTFILKTYQMLEEQNHSNIVSWTAQGTAFIVYNQQQMEKQVLQNFFKHSNYSSFVRQLNLYNFKKVRSNEGQIFKHKCFRKGMKSMLQFIKRRNQEDLIAPAIQEEPTIQVKEEQNLFKECAIDIKETNNKLKEDMKLLQETSSYLIDQMQNLNHFVYNQSVDIEVKFKQVGQMLHAINEELRQENKSETQTNKFLGEYKSIKDDQFQESKIGSPNPYVDYNSTALNPLDYECFIDSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UWI4</accession>
    <name>A0A8S1UWI4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0500110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8169015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UWI4"/>
    <dbReference type="OrthoDB" id="283563at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="110"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8169015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="473" mass="54975" checksum="82E73D6A33A9A017" modified="2022-10-12" version="1">MFPKSCDELFQCRLFKKKFLGTSARYFYVFLDKVVVNKESNRKRTDRVFNIENTNRIIWKYTTEQPIKLKGITLDNGNQQQEYFGEDDVLRELKRHISKLFFQAKVQEEYVAIQVIGQGNYALVLELQHLHSNQKFASKCIDKKKLQAIEQGMESVMNEIQIMRILSPHEQLINLIEVYEGDNNIYLIMDLAQGGSLYKEMKNKLTLYSRQEVQKIMYQILSGLKYIHSKGIMHRDLKPENILFKEKGNLNTLTIADFGLSVKVDSYPYLYPKCGTPGFVAPEIVNMIDKTQSYTTACDIFSAGVIFHILLLGEGLFIGNGHQEILSMNKQFQVDFGRNKYQQLDSDARDLLFKMIAQDAEQRYTATQCLNHTFFQNEMVPQLKLKIQESPTKVTFDHYLDTYNSPDQKLMNKDKKNLSIVTRTPIYAPKASTPELPKQRSIMEELSPISSFSLDQQRNESNKEDQFQFIITK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UZK3</accession>
    <name>A0A8S1UZK3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0510169</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000051">
        <property type="protein sequence ID" value="CAD8169633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UZK3"/>
    <dbReference type="OrthoDB" id="293150at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8169633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="414" mass="50355" checksum="A221ED8EFF2557B5" modified="2022-10-12" version="1">MKPTAKVFEYLNQYNYYLPQNLEHVQKMFYEQMVQHLQLGDSFKNSFISQISQINQKAQREHEFRQLIRDTWFGFVNKTPINIICPMVHQQEVQDVQDFILKVLLEPQSFEIIYVDDLLSVYEAYFQTNKGIVKVYIRNRFRMIISRLICSKFVKQLDTKYQSKVNQFLFQIYLKKIETQDQNILIQTELLTESNFYQKMFEQLFDPSELQGNFSNIIKNVIQILVREIVNVAQSLEILIQFYHLYNEKDKILFEKYDQRLNIDQNKLATEIFLKFKENQDLREAIRIVFQKINLNHSTDYKKLLKDLVSKDIYNRENEQWVCLILREYEYQIQRNRKQPPVQNDFQTYLRHQGFIQKTEKKGEQEFDNPKFNLIIHDQTNFTVYNKMNKQTKKFMQIKEVYHYIQNSIQNFNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V421</accession>
    <name>A0A8S1V421_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0550129</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V421"/>
    <dbReference type="OrthoDB" id="300199at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="537"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="740"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="846"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1057"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="489"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="916"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="489"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8171645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1119" mass="132789" checksum="1A26872126B56EFD" modified="2022-10-12" version="1">MYFLYETIFSYLREESMQLLKSRLKKNFSKQFDQTTLIFEEFYKTEYIIRVLNLQIIKLLKQKCLFYDLLIKEQVSQNQIFDFCFKITIKLCQIENNINQIYQKNSSISILNLKVFYQAEILRNYAKANREHSIIYELESQCKQTSIVLLAKQNKVAFLITKLGDCSYNVKIERASNNAKLFFQTELPQYIESLIPQGIRDYHGQNLHDFFQTGQSYRFKQNARLFILQKNYIQSAESYLDSYNSLSELRFINIILELKDMKGYMILNNQFQIVGINKNLLNLLNFSKSQIDYFQPDEMLYGLNVKILIPQFQILIEENRSVSLIEFYFIKQCYLFDLTKHTMAERFTNMNLLDRYESQIEIQSKYHNNKLSYLILTFDYMKKINVTSLLSNLIECSTKSNQKIELQQKFLEEIKIDSPYDFEDYKDLDVQLFQPELTYQQLNSPQEKSSTNELLFQQKFYNRYHDVSRVSDDVSIFKKNFSKQISMPQFYSDNNNKQKLSDQDEGTPSQKREKYNSTFYDKAEFIDKMVQKRIKNIFVRLAITFLVVSSLLGIVSLLLQTFLIENNFNNYITDVGQLSIKNDILQPMLSFFILRFIIYDKTNLLSLNLITNDQYMNETDFAKQYLKKEFDLFHIQIQELFESKLLQDLLFDRYYQVIYLQDDYTYYYENFSARTFFIKYFDYQQLIYGVYSNNELPKITTPVTFQMLNQKLLFDICSYLSDQVYNNTISRNSKQINDQIIIIACFLSVNFICLLLSNYYYRKFANQRQIFGSMMFNIEKSALESELEYSDYLINQIANHNITQFYTFNQKQKEREIYSLKKQSKTNQSSYQKLIYKKAKIDTSKFLIFIYTMFIGFLTVNLSNTLILSEFIKKYKPTATMHKNTSDLCLNVALIYSEKEQLNNQVFFPYLQDSDFQTMVDQLNQSSAQIKQYQQELMNINLANLLFRSDFVSYFLDQETQDLCQLINAQNNQNIQQICVNSFEGSFLLGIQAVLNHVSKIITQELIAQQFSQRKPINTLEVEGAYLFVEYLQTASKRAMTDFSEQVLKQKSILEKILVSSISFTLILVAIIMIALETNLKLKLKYAVKFIHLIPREVLFLDEAFERKVSMVILKDPNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V8F5</accession>
    <name>A0A8S1V8F5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0580032</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000058">
        <property type="protein sequence ID" value="CAD8172763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V8F5"/>
    <dbReference type="OrthoDB" id="285884at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8172763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="70" mass="8226" checksum="37089C7B97956E36" modified="2022-10-12" version="1">MSFTPPKSSSKFQQMINIPFVDKFQKTFSDVEMQCVQRKLDFGEMDLQSKKSINTFRINKIHKPTIGKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V8Q4</accession>
    <name>A0A8S1V8Q4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">DNA 5'-3' helicase</fullName>
            <ecNumber evidence="17">5.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PPENT_87.1.T0590020</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the helicase family. RAD3/XPD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="17"/>
    <dbReference type="EMBL" id="CAJJDO010000059">
        <property type="protein sequence ID" value="CAD8173254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V8Q4"/>
    <dbReference type="OrthoDB" id="272481at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045951">
        <property type="term" value="P:positive regulation of mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18788">
        <property type="entry name" value="SF2_C_XPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000135">
        <property type="entry name" value="DNA repair helicase RAD3, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000128">
        <property type="entry name" value="Putative DNA repair helicase RAD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010643">
        <property type="entry name" value="HBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006554">
        <property type="entry name" value="Helicase-like_DEXD_c2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010614">
        <property type="entry name" value="RAD3-like_helicase_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013020">
        <property type="entry name" value="Rad3/Chl1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00604">
        <property type="entry name" value="rad3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF1">
        <property type="entry name" value="GENERAL TRANSCRIPTION AND DNA REPAIR FACTOR IIH HELICASE SUBUNIT XPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06733">
        <property type="entry name" value="DEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06777">
        <property type="entry name" value="HBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00488">
        <property type="entry name" value="DEXDc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="15" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="20">
        <location>
            <begin position="7"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="250"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAD8173254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="728" mass="83832" checksum="AEC6A10BB491BE27" modified="2022-10-12" version="1">MKFVIDDIEVIFPYKFLYKEQLEYMQALKQTLDEKGHGILEMPTGTGKTVSLLAFIIAYLAQRPKTIKKLIYCTRTVVEMEKTLEEVRLVLKARKAEGLVDNFTAVGLSSRRNLCINPDVVNQKDRVDAECRKRTAEWVKKGQSETCIFYENFEKSSKDVIANLPNDVYSLNDLRKNGAFSIQCPYFTAREMVKKANIVVYNYLYLIDPGISSLLSKDQIKESVVVFDEAHNIDDVCIEAYTVRLNKPILAEAINNLKIVETQIQSETNEIQNRLNEEYKKLIKNLENKPEGELNQLVIPGQIRKARNFIDFMKRVINSLRKKLKDNSEALKTSHAILHNTKSFTEDIQKEGNLDIDSLKQCGERLNLLLNTLQIAETDKFRPLSTVAQFVMFLVQYQEGFKVIFQSNNYEGTLNEKLMTLACFDSSLAMKNIFTTFQSVILTSGTMSPIEIYPKILDFKPVVAKSIDIELTRNSIQPIIVTMSEDGTELTSEFTLRNNEDVSRNYGNFLLELSENVPDGLIVFMPSYSRMEEWARQWQKDKYLEQISKNKVLFIESKDVNETSSKLQQYRKCCDVGRGAALFSIARGKIAEGIDFEGHYGRCVAVIGFPALNSKDTLILERCKFLEEKFKITKNDFIEFDAMRQTCQCLGRVLRGKQDYGIMIMVDKRFAQKSKLQKMPRWIYKQLDQSRCLNITSESAITVVRDFFRQMAQPFKIADNSYFTQDTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V922</accession>
    <name>A0A8S1V922_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0580166</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000058">
        <property type="protein sequence ID" value="CAD8173003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V922"/>
    <dbReference type="OrthoDB" id="296403at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001689">
        <property type="entry name" value="Ras-related protein Rab-11B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8173003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="201" mass="23059" checksum="3597BC4E9F89E916" modified="2022-10-12" version="1">MEDINYKIVLLGDAGVGKTYILNQYVRGQQPKNNLPTIGIEFATKTVTLQDGGKIQVQIWDTAGQERYRAITKNHFRGAGGALLVYDITKEKTFENLTRWMEELKTAANKDVVMYLVGNKTDLVERQNNSRKVTKEEGQLFAEKNELYFEETSAYRGTNIAQCFEKLIEEMYKKKNLLVTQQQQEQNTQLKAIETPMCSKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VAA3</accession>
    <name>A0A8S1VAA3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0590159</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000059">
        <property type="protein sequence ID" value="CAD8173505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VAA3"/>
    <dbReference type="OrthoDB" id="303590at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="98"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="105"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8173505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="176" mass="20887" checksum="9B8873EB254E6F20" modified="2022-10-12" version="1">MIESFQQQCEQIYSENQEIIKQLNKLKQSALEKNQEILKLQNLVKGVEQEYQPIRDKIQSIKDQFQDSEFNKQIIYDSFDELENIIIRQIAEQFKLNKKSKKTQKKESPQKGKFNNENDQNTINLRKRTIKQQNIDENSEISESSEPIKAQKKKVKNNFEALDKANCLINEIFNFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VAF4</accession>
    <name>A0A8S1VAF4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Kelch motif family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0590148</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000059">
        <property type="protein sequence ID" value="CAD8173483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VAF4"/>
    <dbReference type="OrthoDB" id="284575at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF27">
        <property type="entry name" value="KELCH-LIKE PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8173483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="640" mass="72963" checksum="3AAAA101D933C17B" modified="2022-10-12" version="1">MSATQTQHLIFGSTWPQENMIIKYCSIQQSFQKVPINTGLKINNWSCAIPYKDNEILITGGLGNTNKIISNDVYIFNTQTNQLSQFGQFTQSRYVHQCVNHNDKIYILGGRQYGPDFESVLSHCEVYDGGVWKTISPMTKRRSNFGVFLYNDQIYCIGGFDGKKNTKSIEYYDEAKNIWVRSKLRMPRGLAGFHLIPLENEKIMIVGGMTLQGPSQACIVLNLKNNTCISYSQLNQIRSLFHMFRDEFGNYIVYGGMNKQNTFELFNFENKIWEEFLINMDDNVQEILNQNLRGMSSVQQICRVENYQQIIQQKQLLQIQPDDVVFIFGNDEYPFILPIPKSPTEHLNPLEVPTSLCMYSYMSVCSIDAELPNCRQILLAGGIDSKISQINKKAYLVTIIFPEMKVKVKKVGKLSYHRYAASCVYNAPYVYFIGGRGYQNDVLSLHSTVERFNIDEEEWDQIAPLNEAKCSMTANVIGEKIYVFGGYIGEGQISHKIECYNEQSQEWDVLNIELPFSLEGLSSIVIDDQDLFIFGGKGFDGSKKEIIRFNLDDLESNQNVSGQIVGALQYSRSLPKPILLDDKNVLIIGGYFFDNPEINFGEMFNFDNPGDSNNEYANQIELAILQMGANDSINLFSITN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VAV7</accession>
    <name>A0A8S1VAV7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0560058</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000056">
        <property type="protein sequence ID" value="CAD8172046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VAV7"/>
    <dbReference type="OrthoDB" id="300875at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8172046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="115" mass="13339" checksum="4716494D171989E7" modified="2022-10-12" version="1">MLCKSPKEASLCNSLCCNDLHQCSKYQSECSIYSDNHCIKLNCDTCCLLQENQHSCGTYVQCSKYFDLFYLWCLVILALLLVVLLIIKLRTKFMTARLIRIHRAFAKFNKKTTII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VEJ7</accession>
    <name>A0A8S1VEJ7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DOMON domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0620126</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8175031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VEJ7"/>
    <dbReference type="OrthoDB" id="310734at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030667">
        <property type="term" value="C:secretory granule membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004500">
        <property type="term" value="F:dopamine beta-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042420">
        <property type="term" value="P:dopamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042421">
        <property type="term" value="P:norepinephrine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006589">
        <property type="term" value="P:octopamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09631">
        <property type="entry name" value="DOMON_DOH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000945">
        <property type="entry name" value="DBH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045266">
        <property type="entry name" value="DOH_DOMON"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005018">
        <property type="entry name" value="DOMON_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10157">
        <property type="entry name" value="DOPAMINE BETA HYDROXYLASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10157:SF23">
        <property type="entry name" value="MOXD1 HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03351">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00664">
        <property type="entry name" value="DoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50836">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035942026" description="DOMON domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="DOMON" evidence="2">
        <location>
            <begin position="151"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8175031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="310" mass="34590" checksum="FFB70A250F30568A" modified="2022-10-12" version="1">MTQIIKNILIISLIVLYASAQISLNISSDNSIEKYFITSTEQLVVIGFEGQQEVRDYIFCRMNDCTCEDMVSKGPRSHPQVNSIQQTANVECIDENTITFERKENGYKPYVWNNKPQEEEKRQYQSQKRITQENTVAYTDSDMTASIKVGTELELKWKFNTDDTIEMCVCLNKKAWVGIGFGKGMNGVDMFAINIIDGAAELLDLYSTSENTPPTDSIKDITLVSSSITDTAVKARIKRKLNTGDSKDAVLAKGSSYTWSYASSSSLVMEDHKNNVAEFSITLNESGSQETSQANLLSLIFGLIVLSLII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VES5</accession>
    <name>A0A8S1VES5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0610025</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="289510at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071561">
        <property type="term" value="C:nucleus-vacuole junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034271">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045324">
        <property type="term" value="P:late endosome to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055231">
        <property type="entry name" value="2AA_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045162">
        <property type="entry name" value="Vps15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17583">
        <property type="entry name" value="PHOSPHOINOSITIDE 3-KINASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17583:SF0">
        <property type="entry name" value="PHOSPHOINOSITIDE 3-KINASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22956">
        <property type="entry name" value="VPS15-like_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="3"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="2">
        <location>
            <begin position="952"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="2">
        <location>
            <begin position="1351"/>
            <end position="1384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8174312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1384" mass="163343" checksum="74E045857EB265E0" modified="2022-10-12" version="1">MGNSLIDHRGKLNLMKSFETHNTEQDIGVRQKRLSDNKLFKTMHYKMHHQNLIVKVHLLLKQLTHNELYIHYQNFQYLIEKLEPYPNLMAFRGLINFGKLEKPSPQEMQNECNKDYLAVSRQMLYMTLEERLQSNYKLPICLLHFYTLQLLSAVKTLHQQNLYHGHIRTSNILITSLDYLVLTDFASYKPYYSNEFEVIRNVYESSIQKCTLAPEKYADALEIQYIANMTKQQIYDLQKMDMFSLGCVIYEIYTGESLFTFKQLLSYKRKEREPLIQVDGFIGDLICYLIDLDPNERYSAEMAFNTFQTSICADEIFELLQKICYTINFKGEFMYPDIRIQLFRQILSSTQFKQWNYVMPIPIQFISLNSFNDIDIVSQYPNRFSQIKFNIPQSCDTLAGQIENNENLIIKNNIQNQEDNSIKGNQKQNINELICWKSKSFDRKFLLESVKINNNKRQNEYIIVILWIFNQMRNCRFLQTKLCGLELVEYLLEFIDDSSMYKLIIPNLGLFSEDYEGQTQFYAFSILLDLLRRFNYPHSNKTESLIFQLYIWEQIKTRLQDMNQNPMLALKIGDIAEILHLFNRKEEKNLYLQIIQKKLQDDNQELSILLIQNIKNVLKYYDSSQTIEIMKLLIAQLNKIHLKIPLLEVGFDLLQYYAKTDMSKTLLTCLQLNTRNEMALFRTLRIVTKASEQQLLELDKIKQFIKEISPLILHHNRWVRDEAQQFIIIQLRNFNSLDNYLHLLPHIKKYLKQDVPHLNEEVFTQLIQKPARQSLFINQNQEFNNNSQPLSEREQYNYNNYYHEYILKSKQNLEISSQKLNNNQVLPSTSVKQRLNEFYEAAYKFKFIKQESYNSLQEFIKKDRTLEDYSNFSYDHCKIDLKKFENYQNFNKKYLVSQSSTQWINNLNFNIIINSFIQKSYQLNKSEDKFQRSIQPKIVRQFKLKNQLVTTIPEHDDIVTSLQKHCDQIRFISGSHDGTIRFYDMNTIQEDFTSGSIKQITVFKDQKLIDKVNALCFLEGTDSFIVGTNSGQVSQYKIDQRVTTFPQADSGIRRIVNYDNAFCYVTEKGSLILEDIRSRSQQHLSVNKKCGLASSLIHDNQKTLLSTLNGYLILYDNRCMMQMNIYQLLSKDDQPLPIYNLCEMNRNSLLEGINPNSDKLIGIGYQSLNNEVAFFDLKMNKNTINPILFLHCSQNKNIQMEMPKLKSVLNVEQILNNVNKISTYTQLSDQTIQKFNLNFSSIFVKEQLLQNMWKNYLDGSFQDTTSVTSLLCLNNRGYNQKLENTIITATKDRSVQSWTLNSDINNSELEFQTNVICSPYNSNRKFKQYIKGDVFIIEDHEIRNGPNTSNLIEHQDSVTDMIYLDNENSYLLTASKDHTIKIWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VF11</accession>
    <name>A0A8S1VF11_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">IC97/Casc1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0630080</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNAI7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VF11"/>
    <dbReference type="OrthoDB" id="297923at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048487">
        <property type="term" value="F:beta-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031826">
        <property type="entry name" value="IC97/Casc1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023247">
        <property type="entry name" value="IC97/Dnai7-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20929:SF11">
        <property type="entry name" value="DYNEIN AXONEMAL INTERMEDIATE CHAIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20929">
        <property type="entry name" value="LUNG ADENOMA SUSCEPTIBILITY 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15927">
        <property type="entry name" value="Casc1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IC97/Casc1 N-terminal" evidence="3">
        <location>
            <begin position="79"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="63"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF15927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8175487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="689" mass="82250" checksum="6A9900AB70694865" modified="2022-10-12" version="1">MNIQNYSNKDDKKKTLLKPRIRSSSKEATQQLTQPRIAIQNPPVYRNTTADKHRKHSVDNKKQVKPKKKKKTQKNIQQLDNQEEQKLLEEEQKRIRDLEEFQRQQWEKMTQEERDRLKNEENDRVQIEMIWDKEIHEYKKKCLEFDEWNNYISCNTQIDPRAEKDLNTIIYQLSSNNDDISLNYKQIEYISKIIYIIQIAHQEDLSQSIFNKQDHYFRYIEKLKSIISTKIYEIIKHFYIHYENYLEELNHILKHQYFMLEFNQETQQRILQKIAQKPEDRVKYEILKYSELTNSFKAAFWYCPLSKIGYRIKPIDFSQISNLQIEIPRQLAGQSLIYYITWTNHDPYVQYKQYTQYLPIGGIFQNCCVNLPKLSKKTNGWILKPTFTDSQLQPLKLAQGFQSYSCFFQISNQYYLQDASKLFVVTFQNGQWTQEGIEDLKVNIDTRSVSFSLKNQYPFMLSTLRFADFPFKTWKITSLSPDKVYLNITTQRDINIGIELDSKLSKLVKPTSLAQQHFQQPLPIIELLLKLRNIGINLIAQEEDAICLQTVFKRSEVENQVLLNLMQTLRVYSFKQSKWNQKCGSDQIIIQFIENPTFNQDFEGDDEFQWRNLEFQLNKVALLECNDKSDKLSTKWSKNTVSHANLLILIQQHQITPPELQFYIEEIQNVELRSNLRTMLKVMRLLAIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VHG1</accession>
    <name>A0A8S1VHG1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0600269</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000060">
        <property type="protein sequence ID" value="CAD8174186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VHG1"/>
    <dbReference type="OrthoDB" id="5590282at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000057">
        <property type="entry name" value="Cyclin N-terminal domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000281">
        <property type="entry name" value="Cyclin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0195">Cyclin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="2">
        <location>
            <begin position="336"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8174186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="599" mass="70282" checksum="D7E60CA808BC49B9" modified="2022-10-12" version="1">MTTQWNPYFIPKENYQQLNNISFVRFDENSKQPQNPKSILFNNKIQIQANPKPEDVKYKRNKKQSSCIPFQINIPQQTSNSTLTKVAQPSLKITLHKPKLNEDINLKDSAVWERKDYQNPQVNFESFLNQQNLSRNNSIDSNNKTQFKKNHRQYSHVVQGNSGTYFMISNIPQNKNTLNNTSNSYYSNYENCNSQREEKKIEKLNVPKFIIQDNSSNQLSERTIKKQSPNNNLLKHKKNTRCISMGEQKHLGLTLECDESSITILSDNANSTKSKNIQMNQTFLQNQNDQDSQIGGFSSETLQTLMLQEIEHFPNPDYMVSIQTQINPKVRAILMDWMIDVCSVYHMKRDTYYLAVAYVDSYLSKKSIPKIDVQLLGTASMLIASKMEEVEAKHVSEFEKAANHGYSIDQIYQMEIDVCTVLQWHLNLPTINLWIEFYTNQWDNFNKDIQKKFRANNTASYKLILKLQAYIDCCYLDIQTLNYKPRTLVASFMYLILAIEYQQFTKEQIYYEIPKNSKFILVDSQKSFNKLFTEFVQFSFGFNLVDLIDVIKYASKFIMLDFKFSDTIQDKIIQRHEDLLIYQKYNQSGLLYIENKLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VJH3</accession>
    <name>A0A8S1VJH3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0660063</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000066">
        <property type="protein sequence ID" value="CAD8176911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VJH3"/>
    <dbReference type="OrthoDB" id="288341at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8176911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="147" mass="17488" checksum="86B3A99A6E1DA621" modified="2022-10-12" version="1">MSQKKQEISTWILAHTQLAQALEDAWQEFSKQKSQEDDCNDEQEIKRQQYEKEKNIGQIVQEVWEANLEKLKQNQNQNKTSRSNLFDAECTSYNIHQEKNFGLKDITRWRLSLRDCKLEQTKIGNGIVEIQALEFNEKDQKKKKNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VQ50</accession>
    <name>A0A8S1VQ50_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0680070</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000068">
        <property type="protein sequence ID" value="CAD8177762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VQ50"/>
    <dbReference type="OrthoDB" id="10422062at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8177762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="95" mass="10818" checksum="F0376EABE1DD6B18" modified="2022-10-12" version="1">MMGIIKIKGSHEIVKQIINIVNLFIEMIAIILNFNKFGDVTNAIPNVIKIIINLNILRNLYIQISPIISVYDKKRIANITMSRILQISIISTLQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VT33</accession>
    <name>A0A8S1VT33_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0710006</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000071">
        <property type="protein sequence ID" value="CAD8178959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VT33"/>
    <dbReference type="OrthoDB" id="320105at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8178959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="287" mass="33615" checksum="D20BDD4646BDCA47" modified="2022-10-12" version="1">MEIQSDIQQQKYNTNQVITLKDVGIYKVQNLVFFKKKTAMLNSFISSSDGCLIIICKWNPIFKLKQKPEHNWIIRNYEIKFLQLNSEENQIIFALNSQIHFLSSSKSWFCEQKIQNSLNEIRGLSINPNGNQLNITIRELHNSEWKLKQTLPIIGQNFVLQTISCSVSNQLNLKHYQSTHSIATLENIQNLVKQFVKDQLNKIKQSNLIIVPMFNLKIYYQALMEILQTFYNFLSLKNLQNYECRVVQSIEFKDISIIGTISDNGEFLIIYDPTLNEIQVRENQDQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W214</accession>
    <name>A0A8S1W214_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0830037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000083">
        <property type="protein sequence ID" value="CAD8184388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W214"/>
    <dbReference type="OrthoDB" id="310600at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021258">
        <property type="entry name" value="Epiplasmin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10992">
        <property type="entry name" value="Epiplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8184388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="266" mass="30222" checksum="6725C63F7074B33B" modified="2022-10-12" version="1">MSNHPASTRPPQKAQPVAPQHPIAPSHPIAYSPPRTYAQPVGFASPSYLPVQQSFVGSPVQYVPQQVAVQPVAVQPVSVQPVQQVIKGESRIEYIPYEKSVIEYEEVRQRIQVPREKYVTEYQAIEYQTEYIPQVFYDKVTEYVPVDRFQDRVEYYPVERQVVHQPVQQVVAQPVVQSVVQQVPQYVAPIQSVVQPVYQQPQIQYASYVQPNFAPSRIAPVSYAPPQQYGPPVSQPRRNSPPAKPLPAQKPQPQKEKKSFLDNIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W3D2</accession>
    <name>A0A8S1W3D2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0740207</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000074">
        <property type="protein sequence ID" value="CAD8180606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W3D2"/>
    <dbReference type="OrthoDB" id="304039at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="281"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="478"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8180606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="549" mass="64959" checksum="4446B27E23E15434" modified="2022-10-12" version="1">MRTTPNSVKETASKASTQDYKKQQSQPTVSIPESSQLFHIPQKYCQQRFKPQPTISKSIEEKRYKSNKNNFSEINNRTNSSSKSRISAFTSNQIEDESMYSSQNALKLKKLIGDSQTLNTHLYISKSNLTCDSTSPGVRQNKSGYKKQNDLDIAQINMLREKTLMQKRAYQLQFSQSKELMEQLMNLDKQIQGTVQEQQKKLLYLKSRGLWEPFKDLFNKQFARTSLQYKSSLNLLQIQIREIEQQWQDVTMNITNNIFELMQNSAVLMSYEQDLKLNEMIEQMRRERDIWQNNYKSLEQERDILLETVSQLKQTLEKIKPQQQQQQQQQQQTKKPKDLDQLETHQLKEMAQLMQDKIQEMSDKESKLIKLVLAIKRSGIDIEKIYNEEVLNEDSISEQQNHSIRDKKQMITKLEKSFNDADNSVVNDSDESSFQYNGRLDDESIVESIRRFENRIQNNTYAQESKSSVRMKIDLSKCNQHQQKIQQLQQLQQQQLIKKQQQQQQNNINKLKIPEQDPQGFHQEFMMKFNEFSESWRLQVLKDEKKINQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W3I3</accession>
    <name>A0A8S1W3I3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription factor CBF/NF-Y/archaeal histone domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0830120</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000083">
        <property type="protein sequence ID" value="CAD8184554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W3I3"/>
    <dbReference type="OrthoDB" id="308045at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017054">
        <property type="term" value="C:negative cofactor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042225">
        <property type="entry name" value="Ncb2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46138">
        <property type="entry name" value="PROTEIN DR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46138:SF1">
        <property type="entry name" value="PROTEIN DR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="4">
        <location>
            <begin position="3"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="122"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8184554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="153" mass="18016" checksum="D752BD6CAC6815BA" modified="2022-10-12" version="1">MEQLPKATVQQFIKTSTNGDMKCSNEFVTQVMSMAREYIQLVSDQANTICLEQGKKTISGEFFYKAISKLKLDSQIPHLKEIEEEIKEEVNVKNQNRARFQDEDHLKQLEEQQKQLYEKAKLENSKNMIDDEEQHGKSRKLNIQFEQEDEDEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W4E7</accession>
    <name>A0A8S1W4E7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0800005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8182959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W4E7"/>
    <dbReference type="OrthoDB" id="3214109at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8182959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="221" mass="26469" checksum="655C90040AB5A26B" modified="2022-10-12" version="1">MMHNKINLMILNLRLQNNSMILLKMIQFFNQKDYLEHSKVQKEKYEQELNNKVNLQLYINHFEMGNLIAIKLQNYMLYCLILSEQFFITKTINEKSIEIVLLRILQDLTDNIKLFLSDSIQKQNQYQGEVNPFSAQMLLTNEYDFAKEQNQEVKLYDCKLHLSSDLKDFEIINSKTLQHKLTSIRSIEFDEKNYKSACNHFYQKLLFLIFSPYNDGIYINH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W6D0</accession>
    <name>A0A8S1W6D0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PSI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0810003</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000081">
        <property type="protein sequence ID" value="CAD8183449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="301824at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002895">
        <property type="entry name" value="Paramecium_SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01508">
        <property type="entry name" value="Paramecium_SA"/>
        <property type="match status" value="32"/>
    </dbReference>
    <dbReference type="SMART" id="SM00639">
        <property type="entry name" value="PSA"/>
        <property type="match status" value="34"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035917888" description="PSI domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="2844"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="26"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="77"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="136"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="271"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="335"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="564"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="1914"/>
            <end position="1963"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="2310"/>
            <end position="2362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8183449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2844" mass="292065" checksum="E1F859C115C26A21" modified="2022-10-12" version="1">MNNKFIILSLLLALVASQTYSLTSCTCAQLLSEGDCIKNVSLGCSWDTTKKTCGVSTTPVTPTVTYAAYCDTFAETDCPKAKPCTDCGNYAACAWVESKCTFFTGCTPFAKTLDSECQAISNRCITDGTHCVEIDACSTYKKQLPCVKNAAGSLCYWDTTNNTCVDANTCDKLPATFATDKDCRDVISTCTTKTGGGCVDSGNNCSDQTLEIQCVWNKLKTTACYWDGAACKDRICDNAPTSLTTDDACKTFRTDGTCTTKANGGCVTRTTCAAATIQASCIKNSSGGDCYWTGTACVDKTCANAPTTMTTNSACAGFVTGCITKSGGGCVANGACSVANVQAACVKNSSNFDCIWDTTCKEKTCANAPTTNNTHDLCTSYLSTCTVKSGGGCQNRTCANAPTTMTTNDACEAYLTGNNCITKSGGGCVTNTTCAAITLEAACVKNSSASTCFWDTASSSCKDKTCVNAPATNTTHDLCQAFLNTCTVNSTSAGCVEKTCENSLVLAICDKDTSNRACIWKGKCYKKQCVLASSATTTHADCQTYHSTCTLSNSGTGCVPLPLKCEAITIEAACNLKANGQPCGWNGSQCIDKACSTASKTFTTTSQCTGHISTCVANNPVTVNGSLTIQGCQDLPTTCAARKSSENCEITRVGFPTCLWVSSSTSCVEKSCATASTVGTTGALSAGGFTFSGCQTYLNTCISNNTADGCIAKPSSCSSLVSSNCQNGSKASGDCYWNGSSCVDRTCANIIQTTHNSCNTTFNQCTVNNGGTACQTLATACTSYSTQENCKFTSTNKNCVWTGLACRNATCADAPDTTSYDSDTECLAYPTPSETCTVVYKVGAQGCVSKSANCSDYMTSAQCHKTLTNLTANDDCKWIVDRCYALSSFATGACTTFKGTKTMCEGYRAGCTNIVGAASSASCTLDCTLKTGSGLTFADCQALDSTCSVKKDGTGCIAIQSTCAGYGSTAANCFRSSASGNAGYCTMNTNCQSVTSASECALVTGLTGLDHSKCQLYHSSCTSLKDGTGCQEYKTTCSGYAATNNCATSGQGKCFFDVECLRFTNCASITGTGLTNINCATYDAGSAGATADKCAWSGTACLAVTTVATHCAYVTGTGLTNAICATYNVGCTANRTGTACQEKKATCDLYLTEATCSTSAAAATADKCAWNTAGSACLAVTTVATHCAYVTGTGLTNAICATYNAGCTANRTGTACQEKKATCDLYLTEATCSTSAAAATADKCAWNTAGSACLAVTTVATHCAYVTGTGLTNAICATYNAGCTANRTGTACQEKKATCDLYLTEATCSTSAAAATADKCAWNTAGSACLAVTTVATHCAYVTGTGLTNAICATYNAGCTANRTGTACQEKKATCDLYLTEATCSTSAAAATADKCAWNTAGSACLAVTTVASECAYVTGTGLTNAICATYNAGCTVNRAGTACQEKLATCATYAANQAACSISQAASTAANCAWSGTACVAVTTVASECAYVTGTGLTDAICATYNAGCTVNRAGTACQEKLATCATYAANQAACSISQAASTAANCAWSGTACVAVTTVATDCAFVTGTGLTSAICATYNAGCINLKDGTGCQEAKANCKDYTTSNKCSAQTTSTLSCLWIDNSCYPITDLNCSAITGSGLDHAQCQAYSTGCTSVSDGSKCQDFKSTCEQYPGTTLGCTKTATSKCYLQGSACITISNVATDCAKITGSAGAITYEICQSYNVGCSANRARSACVQQQAQCSGYTSAMTSCYKSGAGLCIASTNTDTACVAATAATTCDAVYLGAGNYSSANCNEMKTGCTNNLTTACVAKTCANAVVTFNHTNCNSYLNTCTVNSGNSACQTMASKCSDQTQASCLYSVEGECVVVGTSCVRKTCDTAATDATRDDDTECSTYQQSCTVARLGACQVRAACASYKSSLQCKFNTSGGKCFWNPTNKTCVDLNCGNIEASSLYDTHNECVVVDATLACTVRATNGAAVQGCMARGACSSYTIEEQCKTNASNGVCVWNTNANLPAPACQDKSCTSAPTSTTTHNDCYAYYNTATVKCTVVATPSNSGGNPTLGGCQQTAACSSYIDKEQCQINANGDPCGWNGTQCADKSCATAPATADYDDDTKCRAYITNKCTVSDSGQGCVEIPATCETMTQKQCYYNKAGDPCYWTGTACITKSCDNAPDATATADECNTYLAGCTLDNVKCKTKVCEDFAFATDALCKQAISTCTTNGTNCVTRGTCFQALSQAGCVTSSTNQQCEWIPAVLNAANVITSPAYCTIKNCSTAPITLTSEAACAGYFTNCTTKNGGGCVTKSTCSAVTIDVACTTALNGTVCAWDSAQNKCRDKDCQDFSGTTHAACQAQRAGCTAGASGKCARVQNCEQTSVRAACIEGTNGPCLWIDKYLNTDGTKGACFRYTSCKSLTWNNDSSCKWISNKCTTNGSNCVGITLCSETNTDGGCVTGYDGACIQSVPALNSSDPKVCKPYTSCADAFYTTHSDCQIASSKCTTNGTTGCIALGSCSSYTAQAGCYFNDKGTLYTSGAITSTGICTWDTTSSSCRDQSCADLTGTTHATCSSQLSTCTSDGTTCLLKGACTSYTTQTACTTAVGSDGACYWELASATNNNTAKCRLLTCADIQNGTATNVCSVALSTCVSNGTACIPKANCSTYTSKIACNSGGLDGICVFTQSTATGAAAGTGTCALMTACTVANNDQTACQAARDRCSWTAASGTGATAVASKCATHTCATNQATNGACTRFLNWDKKTQQVCTLVSGACTATDPSTLSSNDCFLVSGYTYTWNASTSKCGVCTAVVVQPNTTDNNTNTTDNNTTTDSGYILGLSVVLGYLMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WE76</accession>
    <name>A0A8S1WE76_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0820192</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000082">
        <property type="protein sequence ID" value="CAD8184206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WE76"/>
    <dbReference type="OrthoDB" id="296629at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000121">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="2">
        <location>
            <begin position="294"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8184206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="494" mass="57179" checksum="742A1D6D8EC8C169" modified="2022-10-12" version="1">MIEIRRPLSEKNLGNRSSKDLQRPNSSNSQQKQNLQTMKSSIQLQKQNQNFYSKYFSSKDQKENIEIRSNIDRNQSAKQKQTPQDYKKLQDKLLYLENKISSIKSHIDNSQRQSKNSIASKFFSQNTSNNQNSSSVQKCVQIDFTNRGQKEQREFTPQQQNKLKSSTLINNNNEYKSSIEGTIIKKPSLSTFVTQVKAPLSENKTNFKNLKPDSAKSGCSSIKTNFKRKNSQEKTVESSFLYYISSIIKGYMQPEITRPIELIREHLLQTMQASLFQKSVKIVSNFEDKKINLPSTNKKTIVFDLDETLIHCNESIQVPGDIILPIKFPSGEIIEASINIRPYAQQVLQTLHKHFEIIVFTASHSCYANVVIDYLDPHKNVITHRFFRDSCMQTEEGAYIKDLRVIGNRSLNDMVLVDNAAYSFCLQPLNGIPIINYYDNKMDQELLYLQNYLMSLRTVRDVRQYNSQNLKLDKFAQFTDPIDLLQSLHKDYIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WF01</accession>
    <name>A0A8S1WF01_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Lipocalin/cytosolic fatty-acid binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0830101</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000083">
        <property type="protein sequence ID" value="CAD8184516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WF01"/>
    <dbReference type="OrthoDB" id="565904at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd19438">
        <property type="entry name" value="lipocalin_Blc-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.20:FF:000027">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047202">
        <property type="entry name" value="Lipocalin_Blc-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10612">
        <property type="entry name" value="APOLIPOPROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10612:SF34">
        <property type="entry name" value="APOLIPOPROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08212">
        <property type="entry name" value="Lipocalin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035774903" description="Lipocalin/cytosolic fatty-acid binding domain-containing protein" evidence="1">
        <location>
            <begin position="16"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Lipocalin/cytosolic fatty-acid binding" evidence="2">
        <location>
            <begin position="23"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8184516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="187" mass="21319" checksum="5347BC163B7D1C87" modified="2022-10-12" version="1">MNKLILVVLLGLASAQLQSVQTLNVTQYLGNWYEVASSPWVHLTFEKDAYCTRATYGLQSDGNLSVLNMERYGSPQGEVKEITGYAYIPNPEQPGQLKVHLDGAPFEYADYWIVQLGPVKNEQYQWAIVSEPSKFFMWVLARDPVEYNLLYATQVQNTVTNTLQFDGRFNQYVARPWTGCVPYSDTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WFM4</accession>
    <name>A0A8S1WFM4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0890095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000089">
        <property type="protein sequence ID" value="CAD8187475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WFM4"/>
    <dbReference type="OrthoDB" id="289947at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12621">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN CHORD -CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12621:SF7">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="290"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8187475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="480" mass="55771" checksum="8B957B50E39CD226" modified="2022-10-12" version="1">MDFIASFDKFGAVYKPKIKQEQNNYQTVLGGIISILIYLVSFIYFCYKIYQFGKNEVNPIQSIAYEYTENLIITDIGDFLSFQILGTQINPFNKTAQVLVPMIIYQGTEMTDSVFVPYENIYKNEGINFETQLYTPKNLSLQNGEGDYPHKILIGLQVCDSKQHRNCADDTIIDQFFNQNNLLIKTTVITEQYNSFSRSINNMSTELIFAIDRTTTFYQRLIMQAQLLEIDDGPLLPAYNSRWIFSDSLVQTQSLGPSYKTNFTAMFITELSMSFVTQKKVVQYIKVAQVLADVGSIFSSLMLFSIIISSFNEFLMEQELIQQVIEIYYPQFSKLQLKRLFNFGPISEVRFQGKTVDLEQFQKFYKNAKTIAKNKLTVHSNIYQTSRLQFAIQGLIAREALKKLKSQGIKLNLNYDNNTFKSPRENQVQASKSPVIEYQEIEDHINLQFIDEDFDLFEQEDNQQHEKQKIIDEIQEQNLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WHX2</accession>
    <name>A0A8S1WHX2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0900029</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8187759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WHX2"/>
    <dbReference type="OrthoDB" id="298603at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398:SF0">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8187759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="585" mass="68139" checksum="8693C4A41DA77EA9" modified="2022-10-12" version="1">MFGQQKKMLKRMSNYTIDTCDIFGQLPNFRVLEKGRYTSVVGCCMTFLIGSATLIYLITEMIILIDQAEPSVVQSEQQIFNSTQFPLYNDNFTLAITIANQHSEPIVGNLKYYNITVNQCIRQRLVNYINGSVNVTLNCTSLPIAPCTKEDFDNEIQQNFFKNTRLGVVQCLNREFLKRNPPVLQGQISGSVYQYLVIQLSVCKNSTEYQGCASKEEINNVLSAGHYSVYASDYLMQLNKPGQPYSQLINLDINSFSITTSKMLQWTYRIVETHTDDGLLLSKDRVDLNLVKQDRREYSELYNDNYLVYHYIQLDYKESVYRRTYIKLQTILSKIGGIWQLFVILTATILNPLINNLMTISIANELYRFPKNPNSIDKNQNIHINNHIVDPLSTIRQDQIPTQEEKLSKSLYESFLILLGCHKNKRHMFDKVKQEILGQIDIVKIIEKLHQIDMLRFILLNEEQNKLFDLLPKPLLQIEQDQPEDLIVDDLIQEIELQKSFRVINNKKLQTKLKDYYCSFSSLQQEKCKSIVDDKLLHMIDKNIVQFFEQIQISQQRNLNLSPLDPLYRSRIEESIIEPKIQKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WI95</accession>
    <name>A0A8S1WI95_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0920062</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000092">
        <property type="protein sequence ID" value="CAD8188731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WI95"/>
    <dbReference type="OrthoDB" id="10252354at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001436">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="10"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8188731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="350" mass="40754" checksum="5C7E5F52F5F3B762" modified="2022-10-12" version="1">MIITGCGHFYQTIKQLGSGSFGSVYLVKDLNTQYEFACKIISKHLINIYNASQMIQSEISIHSSCNHKNIVKLYSYWEDQNNIYILLEYCSKGHLINPKTQFTEDEVFQIFHQILSGVDYLHQHNIIHRDLKFENVLIHEDGTLKLCDFGWAIKVQQLPVENVMCGTTEYMPPEVVSKQVLDFKVDTWSLGVILYELLHGSFPFIGHDQQELVQNITMNQLIISRRSDISEDLINLIQALLIKNPELRPSVQQIYLCKWVKRCMKTHNLFNHYENEQLKLKVKQKNIALKVVQDVKYLTPNIKIKNSQEDIDVKLTNSINTIQSQSTNTSPKPNKYVDKFFSFIELFKNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WIH5</accession>
    <name>A0A8S1WIH5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0910193</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000091">
        <property type="protein sequence ID" value="CAD8188525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WIH5"/>
    <dbReference type="OrthoDB" id="312188at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="55"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="381"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="507"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8188525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="597" mass="71217" checksum="4A849DF905EB6C76" modified="2022-10-12" version="1">MSKCVNCQQRSGEYICIQPECVKIQGNQMLCFQCYCQSHSKHTPKNFSEVYSLATDNSKKQIQSLEQMIEKNTKELIQLEQFLDNQNQTYIAWNDQQKKSIKECIEQQNNKLQSKIKEAKQILQQQNLDSILNYCLINANQQEVDYQKAQEQSNIERQFQINEITARINFIKDLKFSLSDLSNIFVNTPLPSIPKAQEKSFVKYQQCNIHQKPQKYLCMHNDCISQKSTFPFYCKSCFLIQHNQHNYKIYAKKYKFIKQEQDEKTQLITQEQEKIKSYMQQEFNKNCELIQKQLSEKMKKYDQIKSNMLKIKEQSLENLNKLINFKFIQNNINASLLSKNIQEIKQGYSIEYKRFCQKMSIDLQSESREIFAFTAKLDQKYEKEQNEISLFHQELKSKKEEIDQQKNELKLKSEEIVKLKNQLKSKNEEVYRLENQLRSKNEEINKLENQLNLNKQINSDYYNCFEQLQYLPNSQIYIQKLIDELAKNIKEKINSWIVISMVDRQFLRQFENNFKDIKTQITAAQRNIENIVNKFQQISNSQAKYLPNNYVIQQYNLYNNTVNPYQVYAVDQMKKNQISYKSPSYYQISYSGNPDGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WIM7</accession>
    <name>A0A8S1WIM7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0910166</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000091">
        <property type="protein sequence ID" value="CAD8188487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WIM7"/>
    <dbReference type="OrthoDB" id="298777at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8188487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="539" mass="63397" checksum="F588675BCA1FD302" modified="2022-10-12" version="1">MIHHIFQISDEERAKQEFLIMELGKQEYRDLFNGWVQQKLKFYMLLKKGENVKYIKDGEILKIESIQDMMKRKIICSNLDQQRYLRWETVSDTTQTQCNAYWKGELLQAGGFYNQMEQKEGKWIDLWENFWIKSKVFYKGNYVQGRKKGKWDILLKNQRVGGGAYNEEGQKQGEWTDIDNNFKKKCQVIYNGEYMNGRKCGQWKIIYNGNYLGGGDYNQFGLKNGKWLDLHEDFSEYCQVGFYGEYQNGRKIKKWNIIYRGHKEIEQFNIGNGTYNENGRKKGKWTELHPNFSDYCKVIYVGEYHGGAKEGKWDTIQLEKENKQIKSNLIGGGLYQCGLKIGKWIDLHEKYFISNCGGCKIIYKGEYQNDQKQGRWDIMMNNNQIIGGGNYNEKGLKHDRWIDLHENYSDFSQITYLGEYQNGQKKGMWVINFEGQIIGGGEYDQSGLKNGDWIDLHINFSKFCQVTYKGEYLNGIKCGMWQTCFENNVIGGGIYNEEGKQDGQWVDLHHNFRDLYQVVCIQEYQNGKKIGTPKQIELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WKG6</accession>
    <name>A0A8S1WKG6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0920019</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000092">
        <property type="protein sequence ID" value="CAD8188649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WKG6"/>
    <dbReference type="OrthoDB" id="77931at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8188649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="892" mass="105052" checksum="61FA9B96158CD61E" modified="2022-10-12" version="1">MDNLSLRNILTIKEAELSEESCFTFGIWSKYSPLNIKGFKDQIGMFQSNCYQLINVMEYTTKSLYFIYYVCLEYSTKRIVKTIQFVNDQDQSIQFDKSLDPYEFENIWYQFQLISWPILRRLELIIIKSDKIILKEIITDIKPFKEQQLLLTFGDNLEVIESRIDQITQGMMYSLFPGQIVVYHPLIEIIPISFDFEQQIIEGYSSLTQCNCQNNEQVDLEEIGLNVIDDKIYISENVNCNYYALSGWLKVKDIVQKSEEFTYQFIKMTVFIGKQQIFNENVATFQMFYQISTLSTKIIIKTYTYDFPIAIKEIQDVSQEIETQFQIQKDNLKQWHYLFLQLEDNQFNIKITFYDYQSNIQFYEAQFSVRQFHNCQFKLQYGNIQKNSLNYLKIELRKFQFTNCQYDERISKCHSSCLDCDGSNKNNCLSCPVEQQRIYIPEFKQCLCPYSTIDYDNKCITYEDSNLLLKVKQNINKKCKAGYFELEGDCERCPSRVKADLITCFDCFSQGKDWASKSICGKYIYFPISLSLQNAILYDYSFQYYHDGSDLIICENCLHLDYEQELQNEDGIYKLFLQKFNGFESFCFSLIDFGSEQKCTICNVGFCILCGIQIDGIVCLKCEENYIVVDGLCKFNKQIPYFYQSCQPPYYTTYNRECKICPINYCLYCFEYNYNNNKESNTLSQAEILILEEGNSYVKIGCVLCQDGYIFNFNSGLCVNQVPRIQYCLTSYIKQLDEKCLLSTLNNFEVSRVINNCDDYILNCEICALYYQQVIICINCKIGFVLQEGLCYMNEESKINYSKDIWDLQIKAFLLIYQFDKSQDYPNKEPSKCGNSCLSCKNDNQQYFCEKCMSNHLEDPVRIQYGLYCPECPRLCQVCISRPLEEIKLKTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WLD4</accession>
    <name>A0A8S1WLD4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0940071</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WLD4"/>
    <dbReference type="OrthoDB" id="4928at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381:SF4">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="5">
        <location>
            <begin position="274"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="38"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="93"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="207"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="45"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8189527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="861" mass="101734" checksum="75771CA6D6135644" modified="2022-10-12" version="1">MSKFLQDQDNQTESDSDNQFKQQNSFKSKFFEELQYQISDDDNNFKQQNSIKTVFQQNNPNQSDDERQNHQKIINNISYHSKDKDNTNAQQLDQTDFQKEQNVKRKKKQQKHLNDEKNQQIQQEFIKDSAKKTKKLKVAAQLALEMQEQKRAIQQEERRLELIQYEEEEQFQKEEQIRIDKQNQYKSGFNQHIQIKCGSLKQQQKYFQETLQKQKLLEELIKQEQEQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQSYKKNKNNQAQKEFYTCSILGDFEAGKTTIIRGLINFMKKIGFSVENFGYSYVCQPTQNIQKLFVQMQSYYPVDYQNQKFIFYDRPQHYKNFLTYPNCDIAIVVVDIMQGLDSKSIKDIQYLKLRQIPFIIVLNKFDKCFEQRNIHQLAYQQLKNQTKNCKIIYNEQLKNLLKQLAKKNLKAVQYWEQAESKTHIQIVTTSGAFGIGFEDLLATIVNCLPMYPILNNQPEYLVLNNKDNEQNQSIIECLIINGHLKKGDYLLIAVDGYPTSIRVQNLQEINKEIKMTYFDKNWVSVDEVKTKQAFVRIITEKLQSIFQGCQFKIANSDEAVQEEQEYIKNQFFNSLSNIELDNEGVGAFAQDQASLFKLVQFLQQNKIQICFSNIGIVSKDQILKFVRKCQQKQKQANQKQECILAFGINIQLEIIDFAKQHQIQIISDDNCFSLIGTYQKYIKNISEDKKKQLKDQTIFPCLLNIVELIQISNPMILKVEIVQGILVIGTPLSQFSRKKANVKQIGVVKRIEQNQQALTEARISTGLVTIVVEDNPDMNQFQCQFLNKPQLVSRISRKEIDILKEYYREELTLQDWQLVRELKQYFSIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WPY6</accession>
    <name>A0A8S1WPY6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0980173</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000098">
        <property type="protein sequence ID" value="CAD8191282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WPY6"/>
    <dbReference type="OrthoDB" id="289036at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000454">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000574">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF374">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="206"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="9">
        <location>
            <begin position="465"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8191282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="547" mass="64029" checksum="15327864135CCFD1" modified="2022-10-12" version="1">MYNHYHNLQERISPKNHQINSNFPSDRFHNSKSPLAIDKMLQNPIKKNSQDRTIFNSINFNDDAQLQKTPYFLSIDSFRSRSKSPMQITIQKSPKQSEIPIKKNLANEFYLNWNKLSLEKTLKQKQLINLNINMNDIAQVFQGPQSNINNKQNLKKFENIFQQLLVKYGSTQQLQKTTIKKQQYDDNENDEELKIDTQICVNKSNFAYHFIIGKGGFGKVWKVELKKNRTLYAMKEMSKAKIMAKRSVNSVLNERNLLTQFKHPFLINMVYSFQDRENLYLVMDLLTGGDLRQHLGRLRRFNELQTKFFVSCILLALEYLHNQNVIHRDVKPENIVLDSKGYARLTDLGIARIWRPDNSSDNSGTPGYMAPEVMCRQNHTIAVDYFALGVMTYEFMLGHRPYNGKSRQEIKDQIISKQVQVKRNQIPNDWSLEAADFINKLIQRKQINRLGFNGPEEIKNHIWLQGVPWNKLLKKEIQSPFIPSPIQENLEYNISIETESQDELIIENRLLLKKNSIQNLFVGYNYDQNTKINMRLTKSTSSTLNLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WS45</accession>
    <name>A0A8S1WS45_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0990140</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000099">
        <property type="protein sequence ID" value="CAD8191640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WS45"/>
    <dbReference type="OrthoDB" id="184675at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03213">
        <property type="entry name" value="ABCG_EPDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002483">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF139">
        <property type="entry name" value="PROTEIN SCARLET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="363"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="392"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="435"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="475"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="504"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="578"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="33"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8191640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="604" mass="68141" checksum="852C4AA263DC3D19" modified="2022-10-12" version="1">MQEIEILPQIEAGRLNDTVQSAKSLRCRRVVDIAFQNIEYQVVDQKGNKREILRNLDGYCPGGEVTAILGASGAGKTSLLNILAARVQSSKSVKLKGKVLANQVEYDSETFSNFAAYVMQNDVLFETLTPREALQFVADLKYTDSELKQSRVEDTIKTMKLERCQNAIIGGPSLKGISGGERKRTSIGFELVTNPSCILLDEPTSGLDSFTAFQIIYELQLLAHEQDRTIVFTIHQPSSDIYLLFDRIMLLVQGKFIYQGPRTKLVGYFKGIGFSCPDHSNPMDFMMSIMHQESQINIDNFPIYFDQYEKQLHKPVLDEIKESSKTELVYKQVETSTAFQINLIAKRAIKGFLRDKMIVKQRVGMAIFLGLLLGYSYYGIGEDSGTLADYTSMSGCMFFLCINLTMSSLFPVVLQFSTERDVFLREENSKLYTTFSYFMGKSFVEIPFCLITPILQELILYWMIGLNTRDGGVVIAHIFIAILTCLNGNSMGLMAGCAFNDIKVATSIVPLILLPLIIFSGFFANSKQFFVWIGWIQYISPVKYSFEALATNEFEGRNYEFGDPIETLGFEVGLWESVAILIAFTVAIRLGAYMFLKMLRQKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WVH5</accession>
    <name>A0A8S1WVH5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CDT1 Geminin-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1000185</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000100">
        <property type="protein sequence ID" value="CAD8192119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WVH5"/>
    <dbReference type="OrthoDB" id="341730at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014939">
        <property type="entry name" value="CDT1_Gemini-bd-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08839">
        <property type="entry name" value="CDT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01075">
        <property type="entry name" value="CDT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CDT1 Geminin-binding" evidence="1">
        <location>
            <begin position="114"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8192119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="391" mass="46577" checksum="C948BFDAF3FE86FA" modified="2022-10-12" version="1">METNYQKETKRPQQIIKMAKLHNNYKEPDDTYEPKRRVKIQSTFNSIDNVQQLNEDSSTFNGIDYFENLQQKKSNEKKKTPLKLESVSSILAKVSLRERFSNLHLITSIDKMLMHSKYSNLCHFFKEIDEILLFLQNQQKPPFFNTIYELLSHNSKIKITQQIIQQILEIFPNAYDVNWSLNEKLIREDQSDLMIKANIKSHVQLVDRLTYFQQLMFKYIQKNGEYIGLAILPKNPFQQILKDQFEMNNSLKKEIQQQTYENEIQSEIYKEPIYSKLSQNLIEKLKSKKKPEIVLNDCKTPKQQLIALSQLIHQYYHIRDVSNMFLINVVKYSFNSLKHFILDENQIKAYIDQLITLCPYWMQLIENQNGSILRLNKEIDIPSIIRSIEYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WY63</accession>
    <name>A0A8S1WY63_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1040137</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WY63"/>
    <dbReference type="OrthoDB" id="294251at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000159">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000108">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF9">
        <property type="entry name" value="GTPASE ACTIVATING PROTEIN AND CENTROSOME-ASSOCIATED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="1">
        <location>
            <begin position="1"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8193541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="360" mass="42630" checksum="86241641EFF5BD4D" modified="2022-10-12" version="1">MKSILNQSKQNLMLHVANSIIKNAKNDFTVKDCDVPTINKIPASKRIQTKPKENALSEYNVITRCVTEQRKSSVTETSINEQKEQKRIPSYCMKWLNYKYQKKEFVQLEQQFQKILQIQPEKDVVHQIERDLSRTIKAENHFKEIRNILTAFSVYDPNIGYVQGMNYIASILFNHAKQEWIAFWLFVNLIEQMEIRDIFQLSLNAINKYSKILDFLISRFLPKAYENLCLKQVNTELYIQQWILSLLLQFIPFEYTQMYLDGLFKQGISYFYMIALTIIKVFDKAIQEEDQIEILILLTSKQYSQLKWPSILALQWDINPGELRVLLDCFDSDTNTFNKPKFQTHFSQLTTNFFQFIKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WZ14</accession>
    <name>A0A8S1WZ14_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0980115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000098">
        <property type="protein sequence ID" value="CAD8191166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WZ14"/>
    <dbReference type="OrthoDB" id="307142at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035727057" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8191166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="16134" checksum="162CE17F0725A853" modified="2022-10-12" version="1">MILYNHKIIMMLLLLRINFVLNNVVKNEIHQIFSNTIVKTKKCLFIPHSAMIVFAQSFLQKQLKSVNKFKMSIIHQKVVPILRVVFLLIQKSKSLTENLPLNLLNQKLSSIINNLLSLSLILNNEYKQINNYTKIHNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X5Y6</accession>
    <name>A0A8S1X5Y6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4378 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1120088</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000112">
        <property type="protein sequence ID" value="CAD8196360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X5Y6"/>
    <dbReference type="OrthoDB" id="304761at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025486">
        <property type="entry name" value="DUF4378"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14309">
        <property type="entry name" value="DUF4378"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4378" evidence="2">
        <location>
            <begin position="645"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8196360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="812" mass="95307" checksum="8719712DB5C37111" modified="2022-10-12" version="1">MQNNSPIKGRRISNQIKLSGKLMKKKMASEVDIIGPSDQDTSAVDPIQIVQYDNLKSHIKQSQSELKVGKSTDKIKKKKSLDRNAAIKKSQQLYQKKKKSTKIKKSVSAERVQSQTVEFSSPEYVRRKKAATFFNEKPSSIQEHRDRLGLSFLNKIGTRRIDQILERNRMSSIEHSHHQPLTPERHQFPQQLKHYMKEKKKFYSQLESVKQQSFAEKRKHIDENLRSLAEIAKRNSLSNSRSPPRDTSNKASAKPLMLSYVANRKSTSRKSGGPKSLKEQFKQLEARYKTLKSNSDGISMRESRNYEQEMKDHAAICIQKWFRGHKIRKISKQKKDKKIIKLQQFQKLKNQEITRWNNLREFLEKLQIKQDMIVISELIENLIKHAEFNKINVFTDNFEMPQIKLKPSKLIVERRDVFPTQIGQILQLRGQLIKEREQREKNILKDLLIQERISPRSFGVKEQEIMKWGEKELELLEHNKQAIKEGWQKAYDTIQKTQKDLKFVQKLGVDNFNKMMPLSLSQSNLLKNQLNDLKINLLRVSYSEENLLNPQKKIPQQDQEQQLVSYNSNQSNKTLSSYIQGLEQGQIENLQYVQTHVGYIRQYLEDIKLIVRNHYQNQFLQIVNLGIGPSPFEILRFFRLTEEMIEQSVDGGFIHQAVLGLEIFTQKEKISQEEVCNNEMERIHNKAIFDAYNESLDYFRPFGIKGKPLPWRRNVISRQIKDLDETLNQSASKVVQWAETLCGILLPQGSQIDNDILPQVREERLDKLLKQEVLENDDRWQEFDEEHTEVTLELSELVFNHLITEVIQELRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X7P2</accession>
    <name>A0A8S1X7P2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1140159</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000114">
        <property type="protein sequence ID" value="CAD8197185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X7P2"/>
    <dbReference type="OrthoDB" id="284525at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="2">
        <location>
            <begin position="53"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8197185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="242" mass="28826" checksum="AA2B4034C868EA05" modified="2022-10-12" version="1">MIKFFGIVIYIFFILSVVGPIYSFLTLQPKRAQEFWSRLAVKTLRLQFRYLPNSQQVQTNKRTMLLFNHRTQGDFFIHDVVSQYSANFLARWMVAVAFPMLFIFQQLFTQSVWFFRRGGNDLNNFFKWIDNKFDKATRPNLLVYPEGHRMHESDKVGKMKTGMLQYAYDRKIDTQIIIVLGIEKAFNEKKFHASLGPTQIAIKVDEIIKPDKFATFELFIEHIQAMFKKNFEETYDYQYKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X9S2</accession>
    <name>A0A8S1X9S2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1160091</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000116">
        <property type="protein sequence ID" value="CAD8197744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X9S2"/>
    <dbReference type="OrthoDB" id="311853at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="4">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="4">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="4">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="1">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="1">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="1">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="359"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="439"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="317"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="439"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="457"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8197744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="527" mass="63114" checksum="AF3CBC1778F78848" modified="2022-10-12" version="1">MNQLEDGEVPTNNEPKKNGASIYSSTYRTKVCEHFKKGSCIKGNKCSYMHPKELQNVTRICKYYLGQGCQNQQQCQYSHDLSKYQCKFFFAMSNCKGQNCRFSHDLWENEIAKQQWAADNDQFLKDCYQRRGTTGLGAAYDEQYKMKVFEQQKVNPNQRNYPFYPNNYMATQPQSQMPNNILKQISPQIPQGTTKLPINNDLDPISDEEQQEEINKLNIYEQQNPYQILQNNITPIPQHQPFSINNQYIQQRIPINQQNQFIYPPTVLPNQQQQQQMFQQQAYQQMKYYHQQSQNNMYMNQYHPQQPFQLQQQQPLIEDQKEQILQKQQIIQQQKQQQLQQLQQQQQIQQQHQQQQFLQQPQQLQQPQQPQQSQQPQQPQQSQQQQQQQLQQQQQQQQQQQQQQQQQQQQPQQQPQQTQQQPQQQQFEQQVNNGKLENKEKIESKQQQKKSKEQQQKKIKKQKSKFNNKKKRETIKQIKDRLKKQKNKIKTIQIPPEIPTESYKVGSISKHLGITIRKPVLQYFLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XCG2</accession>
    <name>A0A8S1XCG2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1190022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000119">
        <property type="protein sequence ID" value="CAD8198482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XCG2"/>
    <dbReference type="OrthoDB" id="10263206at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF661">
        <property type="entry name" value="GH16847P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="1">
        <location>
            <begin position="1"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8198482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="316" mass="38107" checksum="45D543CAE6DC5FFB" modified="2022-10-12" version="1">MQQYSPRAIIWMTYLNYLPQDQAQQLLFLKKQQQLYNSYLDEFIKTEHLEIFNILNNIDSDQNSLSFSEFLNIKTQVINSSMDYNEYIQNEHLYELIEKDVPRTLPNQSILKEQTNAKFSYYFFTDQYKRRKQQSNTFPTHSDILIRILYIYGKLNPAIKYMQGMSDLLAPLYLIIKNEADTFFCFTKIMAQLKDAYISTLDFTNTGIRGLLLKFEKQFQQKEPKLYNYLHNLGIHPYMYGYRWIITCMTREFYLDQIYQIWDLMLHDRNMHDFIIKFAIAILKYLKPQLIQADFKLAFDILIYSEKDVNQILNFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XEU9</accession>
    <name>A0A8S1XEU9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1210152</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000121">
        <property type="protein sequence ID" value="CAD8199383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XEU9"/>
    <dbReference type="OrthoDB" id="298484at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8199383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="67" mass="7640" checksum="3B6B91E545E93559" modified="2022-10-12" version="1">MGICHGGTKYNNGQLKQIMAKAKKSEDIAIILQETTDIYTTFNPYKNPILNRRLREQSTPTQSNVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XHS0</accession>
    <name>A0A8S1XHS0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1240088</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000124">
        <property type="protein sequence ID" value="CAD8200374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XHS0"/>
    <dbReference type="OrthoDB" id="296073at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001943">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000009">
        <property type="entry name" value="PP2A_PR55"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11871">
        <property type="entry name" value="PROTEIN PHOSPHATASE PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037309">
        <property type="entry name" value="PP2A_PR55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8200374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="416" mass="47758" checksum="56B8DB5EA1D163F0" modified="2022-10-12" version="1">MKKQDWKFMQVFGDKASSDNVSDEDIISAVQFERTGRFLGLGDKAGRLIIFEVPQNKKRDKAEYQYLTELQSHTREFDFLKSTDIEEKINQIQWLRAQGKNMYVLSTNDKTVKLWKVSEKNVTKVIKPSGKDLAMPKLQVVESGLIPSVRKVFPNLHNYHINSLTASNNEEFVLTSDDLKVYLWSIEQPSKAFVAVDLKPENLDELSEVITSSTFHPTLDNQFLYTTSKGIIKLCDMRKSGICDNTAITMTEPEDPAKKNFFTEIVTSISDACFSRNGKYIFSRDFLTVKVWDIAMTNKPVATVQVFEPLKSKLCDLYENECIFDKFSIQSTLDSNSFVTGNFNSTFHIVDRQGECNSQYELNFNKKTVVRQIPPKYFENLGSSYDFNRKVLKISMCQTQNLFAIACLNCLYFYTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XIK1</accession>
    <name>A0A8S1XIK1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1260079</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000126">
        <property type="protein sequence ID" value="CAD8200965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XIK1"/>
    <dbReference type="OrthoDB" id="296996at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="110"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="404"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="152"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="419"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8200965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="464" mass="55469" checksum="DD57DB5AD50955EA" modified="2022-10-12" version="1">MSQQKRREQLEKISNSQKEEQDKNRRDELEKEFLKCQKRGQMHTNSMMNWINKHQNKADPKAKEMYRYLPEERRKHIELTLLFEKFDNDGSKALDFIEVFRMFTKYGIFITKKELLDFFKVVDQNKDFSLNLQEFKACYLDSAARDIFAKIMKQLIERTQLTEEEINAALNQMNELNNQNNNNQNQNPIPALPKSFQGVISLLTYLNTRDEIKDKLTSKSVNIYEQINNLKQLIELSNKIDVVQKVEIERKIKIKKSSEDRLNKFKNQLKQIKDRAIQTGQMMAEEELRQKNSAFLITVLPQRKYKYNYKSENKFKKIIPNKQEELHLPRYSDQKVNQFYEYMNINIPDQLKNINNRIWTKLFENNQQLDIILQKNRKNQLESQRTDRLKQKIKQTLDNLQANFETDFDSEANRKPTLESPQEKQSTQFPNFFTSRNLMSQTSEDFRTAKTQRMPPGSSKLSFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XKJ3</accession>
    <name>A0A8S1XKJ3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Insulin-like growth factor binding protein, N-terminal</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1290126</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000129">
        <property type="protein sequence ID" value="CAD8201960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="304690at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8201960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="604" mass="69927" checksum="CE0EB933AC8A258F" modified="2022-10-12" version="1">MLLICILISITNQQTLFYSAFSDTILTQTDNWRFLNINPSNIITQCGSEYIIGGFERFAQSQAATKILLLPPHYRMKISMTLYIIDSWDLNEYFQVYADQNLVYNVAYSGLQGTGHICGQQQTYTKDQIHNIQFEFDHTGLTTYLYLTSTLDNSAWDESWGFKNFKLFLYLCPPGCLVCIQSDSEFDCKSWVLTHTAYTEIDYTLFITDGWTISYGDVNKQQCFMIPTICGYEVCGKDTVLSLNLINIPTHSQMKIKLKYLKIDSWEVSDYVQLKVDGELILDQQLSQLNEYLYGICGTTQKEVFVNIELTFPHSITSARIKILNTLDQAFSDESFGIRDIQIFIKQSLCGDQVVELQEECDDGNLFPFDGCFNCMFSCVDGCSECRDMICLSCYEGWIYLEYEQICEKEIIQEYSLITILELQTQFDCFPPIDNCQVCLLGKCILCQNDFFFNQFNDKCEQICFDQINSYYELCDEYQLQPFCNRCQIKCNDHCLNCNQGICQVCENDFILENNYCQCQNICGNQIALYTLEECVCDLYCEECEDGICYQCNNYFKLFNNQCISTCGDLIVQEHEDCDDGNNIEFDGCFYCQYSCPXNIFNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XLC9</accession>
    <name>A0A8S1XLC9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1270068</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sel-1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000127">
        <property type="protein sequence ID" value="CAD8201292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XLC9"/>
    <dbReference type="OrthoDB" id="272077at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050767">
        <property type="entry name" value="Sel1_AlgK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11102">
        <property type="entry name" value="SEL-1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11102:SF147">
        <property type="entry name" value="SEL1L ADAPTOR SUBUNIT OF ERAD E3 UBIQUITIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="681"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="683"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="715"/>
            <end position="731"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8201292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="731" mass="85348" checksum="5A4FB52869278220" modified="2022-10-12" version="1">MNQQQFIHSSRLSTEIGDEKMLLHKDKLLQKIIEQVQLLMSNTNQAMEGDQQFYDGFKYGIAHQQISDMMSQVQMSSNIDQPRRVFSQQVFTKMSQILKSYSTQQHKVTIKINQQLHYLARDTIRTIQEAFEACQQVDKVQLCGKDASFKQYDKYIRQLDQLGFFWEQLHSLENRTITINKETISSEISQYLDQWLDTLIQRFDFKQTSQRLQTPQQIKSIQASKVQPQVDHRLRNAHELFAGLGREKDQIAALQLYTKLAEENNATAQAIMGQICLEGITGPKDYDQAFSYFKKSADQRNTFSLYHTSKLVLEGKVYDKFSDKDDNNTIVSKYSSEAYNRNYDCTFALTLLKRAAELDHLESMIYLGDLYSNGLQLQDYTLEKDYSNAEFYYKQAKNKNSTQAMHKLALLYQTMCKQPLYKNRSQLIQPLLNQAKNQDYLPAFYDLGLLLYQGLQDEVEQNQVMADLIFEQGCMKGDLKCAQKLLSLRFQALRKNEVAIDDFFSLLDQIEENNKDWTITYYMRGKVYEKGVQVEKNLKKAQEQYRLGYMKGCQKCRAQLDKYQKDTIPTDNNPTNSQQSSLNQRQTINGFIEQVRQSEWRRPFQKQRQVSIGNVQQIFNNIDNDVQSTVQDRYTRMTISITRPKNQTDDRKRGVSASELPLQDIGRSILLSQMKSPEKIHQHSQFWSPRLPSQQSQAGSQITNSTTKKRFNLNKIRQNNYPSNQFENVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XPX5</accession>
    <name>A0A8S1XPX5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1330055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000133">
        <property type="protein sequence ID" value="CAD8203153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XPX5"/>
    <dbReference type="OrthoDB" id="291793at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="48"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8203153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="173" mass="20980" checksum="7A45B849F7182E9B" modified="2022-10-12" version="1">MSYFEKWGYDDIRTKELDDLCEIKHQGIQSFQDIALVKQQLELMKFQSSQLREQVQVKRENLQQIKELTKNLEVLLDEESQDIEKKIEVERKTNNELNQYNIKKQQIPHLEDLQQYIIPLNERTQLYQQIQNKRKELLGDNYTLMPPQTFKDALKENYDLKQQLHELKMKIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XSC5</accession>
    <name>A0A8S1XSC5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GP-PDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T1340079</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycerophosphoryl diester phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000134">
        <property type="protein sequence ID" value="CAD8203598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XSC5"/>
    <dbReference type="OrthoDB" id="311925at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08612">
        <property type="entry name" value="GDPD_GDE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052271">
        <property type="entry name" value="GDPD-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42758">
        <property type="entry name" value="PHOSPHATIDYLGLYCEROL PHOSPHOLIPASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42758:SF2">
        <property type="entry name" value="PHOSPHATIDYLGLYCEROL PHOSPHOLIPASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="9">
        <location>
            <begin position="43"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8203598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="330" mass="39212" checksum="D6ED248D9A3E7F35" modified="2022-10-12" version="1">MNYLFYGFIGYWALSLTFFVFPYKKKNVFKHQKYQKYRQRKSIIHISHRGGCRENLENTMQAFQHAYDLGTDCLEMDLCMTKDKKIVVLHDSSLLRMCGVEAHVKEYNYADLPKFLERVRLDFAPNDYISTSTCKTPYMPLFEDVLKKFPDVLINVEIKTPDPEVVQVVNDLILKYQRQDTIIWGARFQKQNELLKSINPSIPRFFTVEGILRVYLLYMTGLLPFFDIPDDSMQAPLYTDDFYLWKLQVANSTSEKFQQMLFHNFIWFLGLMSKPLFYHLHKRGIFVYYWVLNNEKEYERALKAGCHGIMTDCPTVLKQYLIKNKLYGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XT16</accession>
    <name>A0A8S1XT16_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1340121</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000134">
        <property type="protein sequence ID" value="CAD8203682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XT16"/>
    <dbReference type="OrthoDB" id="300021at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8203682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="242" mass="28420" checksum="AD1E563CA8726B1F" modified="2022-10-12" version="1">MADEEICQNIALGDCSNNDAQLREAFYSYPSFQNLFKLYNEKYLKLAKFLEDDNINQEVKKIQDEGEFNSQDEYYEAIQKIENNLKEIKDIPEQKQIFTIKIAQSLIDEYFKQLKNCKHQLFGLIYSSNKNNENIGHFLIMKNQQSESILNVQSENEVTKTFKENELTKLCAILGCDEFFNEERVKQVSSLSLKTQLYLLKFNNLTGLISCQNYVNGEAQQIKIQDITVKHFEGVRQYWIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XU59</accession>
    <name>A0A8S1XU59_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1390047</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000139">
        <property type="protein sequence ID" value="CAD8204921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XU59"/>
    <dbReference type="OrthoDB" id="300247at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8204921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="191" mass="22072" checksum="34DC28CF9603A39F" modified="2022-10-12" version="1">MQRSWLEQPQRTLDINFDIKSIRNQVKDRCVPVLQYQTDSAQKSFNSYRKESLPTVPSFENLQAKIRDFSYTKLNQAQSFQQRNQITDKSITYSPQKAVGLQKKINSSLGSRNNTNYQSSRQQQSPAYQSRPIELSYRSQQRRNLSDNLQLNEIVSMRNKIESSQASRSLLGTNYVSELVKLAQAITNALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XUU7</accession>
    <name>A0A8S1XUU7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1370054</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000137">
        <property type="protein sequence ID" value="CAD8204412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XUU7"/>
    <dbReference type="OrthoDB" id="291592at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06627">
        <property type="entry name" value="STKc_Cdc7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF4">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="137"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8204412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="976" mass="111728" checksum="E647B9DE454ED261" modified="2022-10-12" version="1">MRFNRFDQGRNLIQLRQTISMFSISSRRLSLFIGILIEESYFSTPIQQSGFQFTDLSQLLYSLTLVSDQIHQLNQFMANNTLDTQSTIFVSLFTFATLILLTCILYLHASLQSLLYIYIKQYQMSQLSRQLKKIGNYQLGDLLGRGSIGTVYKGLNLELGTLVAIKQVSRATLKEDQYKALQQEIYLLKKLKHENIVKYIDCIETDQFLNIILEYIESGSLASILKKFGSFPESLVAIYVKQVLKGLEYLHQQGIVHRDIKGANILTTKDGTVKLADFGVATTLSEDTTQSNNIVGTPYWMAPEVIEMSGNLSTSCDIWSLGCTVIELLTGNPPYFDRLQYAAMFQIVQRDCPPLPEGISNECKDFLIQCFQKDPTLRDDATTMLKHIWITKSWHIVQGPNLPEEVTNTIRNHIVDQSNFGTFITDSFIEQVPQLVQCQSPRKIDKSSAVRDSQLDSLRQLKLRTSHQIKQNGFWGQNEDGKSMREINELITQLTEQNDNSQIIKILELLKDTIPNDKCKEYFIHTGFTSLLEIIEKYMPLYCENNSQDQSLQILKLIFELINIVVDQNPQLLELSCYLGAATFYQQITSFEFHKELRVEAAYFLGQLTYFKEGYLLSIGGLDNLLELIDTSDISNNIDLIGLALDTIILLYDLNTISQRNLSRLLTSKFITYRLVLIAEQIQNIDVNLMIKSLNILVILAKCEDKIVKQSLCEKEVITRLATFLNQNQEQALLKTIKIFRYLSTEPNLQPHLDGFAIIPRALTLLKKDLPSQGPMIADILRIIFYLTKLNHDKQEELCLYEGVPLLLELFNLPSEQENQISMKKAAISILCSLATSSERCKQKLQENGGITIYVGLLTTNLNITKLLDTIVKLIELDQTQRIPLSKNIDILVSYFKNNTVPMYPTLVKVLPKEFGLSLLFRKQLLELLIQKDQSQLNLKHLLQILYYLLESDSIKIKDMKNMVWLFKALLSKLRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XVF8</accession>
    <name>A0A8S1XVF8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1400053</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000140">
        <property type="protein sequence ID" value="CAD8205240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XVF8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="210"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8205240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="278" mass="33509" checksum="FC046027EA232716" modified="2022-10-12" version="1">MTENTTVTNVNWADFSDDEDQVQPIVEKTSEQEIKPTVQSLEQNKEFKKQTKKPYHKIINQNGLSQKSYKRNKQKNIKRVISSIRGKKNQTKKKIFKVQCIIVMMKMNNKNNRQYKRKLQPNKHQNNKLSKKKNKQNNMKYLNKNSNRQINSNNQKSNNKKRKRQKIQMQFQMNQVLLKKKMLKQKEFKIKKRKKKNQNKDLIHTEKQSEQPQQQQQLVLQQTDQKNNHDKVDIKKVLAEKAKKNQIGTYQVDKREQNKKLKRETKNRKKNKKQDLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XWC8</accession>
    <name>A0A8S1XWC8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1410082</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000141">
        <property type="protein sequence ID" value="CAD8205643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XWC8"/>
    <dbReference type="OrthoDB" id="304370at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8205643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="130" mass="14445" checksum="8BE37EB546277CA5" modified="2022-10-12" version="1">MGSCCMNKMDMETLNTNLSSEISKKIVINSPIVKNIIGKQLEIIEIPSYTILDSVFEYEVLDNFESEDSQSSEKKRFRNGKGILKQVTAKSHGSGGSSASSSKKVSFCRTTTFFEKKSRKGLMLESQTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XWK8</accession>
    <name>A0A8S1XWK8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T1410132</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000141">
        <property type="protein sequence ID" value="CAD8205743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XWK8"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20621">
        <property type="entry name" value="CYP5011A1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000091">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF50">
        <property type="entry name" value="BIFUNCTIONAL ALBAFLAVENONE MONOOXYGENASE_TERPENE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 7" id="KW-0349">Heme</keyword>
    <keyword evidence="5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="240"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8205743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="508" mass="60250" checksum="E6A45B173683B755" modified="2022-10-12" version="1">MIFFIIIIIVVYVLYRIILKPLIPLLILKLQFGKQAYLWYAPIWGLLKYNFRSLKQNGDFFDDLRKRMRQNPKVKMLLTNFLDKPFINFVDSEYVKKMYLDHQNFPKFNLLRYDNLIDKGLLMAEGEEWKHQRNLLGSAFTFEKLKSRIPMINQIVKERCILDPKTNSFDFISWITGEVVIHSFFGEIAKGLIINGKPGQVEIVNLVADIMLQRFKNTYTQIKLMILGNKGWRILATKKEKDINNRVDMLRNTVKQLIEKRLEQLQVENQREKQKDQFFLDIYLNELLKQQQDNERNKINIDLENVLQQFLTLFFAGTDTTATMTITCLVYLAKYPDIQNELLQEIQDLIQDQDVQDNHLTKFVKMNAFINEVFRLRNPAFSPFVRLVQKDMQFLDIKLKKGWMVVQRHDIPVIRHEHFSNPEEFDYKRWLINQGNIQSDNGFVHIPFGAGGRNCIGQHMALMELKIILCHIIRKFQVSLNPDVQIKFGIRFLYGAEPENCLIYKERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XWL2</accession>
    <name>A0A8S1XWL2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1380121</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000138">
        <property type="protein sequence ID" value="CAD8204832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XWL2"/>
    <dbReference type="OrthoDB" id="626167at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="76"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8204832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="176" mass="20776" checksum="0F662A0C1FEDF427" modified="2022-10-12" version="1">MQDFAMRKQSNETAILIWNIIISISYLIKKNIVMLQFVLIKQFNGNLIFQWHIIIKKYSDAIECFDDAILLDSNFAIAYNNKGISLEKLKKYDESLANFSYALKIDSKYSQAYMNKGNIYQTNKVMYYETSKVKLKISKMKKKQQQAIDTYMQAISYCQLDENEFKRLILEPKNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y0X2</accession>
    <name>A0A8S1Y0X2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1470043</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000147">
        <property type="protein sequence ID" value="CAD8207370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y0X2"/>
    <dbReference type="OrthoDB" id="287383at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8207370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="178" mass="20931" checksum="26314E16F8E9D803" modified="2022-10-12" version="1">MYNHSNISYTNTNNMNRQELQEFRNYVRKTAQAKMDGTEDKQRQIAKTFHMDHKNLMSLNQSPLQSKKLHVDHDISQHHHLASLSDWKKFDRSTSQSFVSFDANTWKEDQNSMRQRKLQTPSLNAADLIKVNRTLSGLSTKEIQNLSGQYYHQIQELEHTVSSMLRRIDFIQASKSNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y2V6</accession>
    <name>A0A8S1Y2V6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1500033</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000150">
        <property type="protein sequence ID" value="CAD8208239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y2V6"/>
    <dbReference type="OrthoDB" id="283303at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8208239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="88" mass="10163" checksum="A8828D5924008533" modified="2022-10-12" version="1">MGITCSQPKKSSLTITISQENEDPNYEIKKLLSEERITKLRKTFPSPTKTYESYFLSQINNDKTMNINRCCDEEKFHKSSKLGVPLIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YE94</accession>
    <name>A0A8S1YE94_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Histone deacetylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1660054</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000166">
        <property type="protein sequence ID" value="CAD8212039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YE94"/>
    <dbReference type="OrthoDB" id="437693at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004407">
        <property type="term" value="F:histone deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09993">
        <property type="entry name" value="HDAC_classIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044150">
        <property type="entry name" value="HDAC_classIV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF23">
        <property type="entry name" value="HISTONE DEACETYLASE 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Histone deacetylase" evidence="3">
        <location>
            <begin position="153"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8212039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="366" mass="42053" checksum="2F9F9D2933C93E8D" modified="2022-10-12" version="1">MQLYTFIDWFFTIGSIIIAILLGIYVNGAACLLGLFFYLIYPFIIEFCYNLLSVKQEISITNMPEIPIIYHDNYNITACGIEKQHPFDSCKYRNVYRQIRQKIKAQHFTPQMLSRGTFLYLGMSKWYLLKICYSAYISTLIELHVFFLPGVFLRSFLLDSMLLATSGSVYGAKLALEKGWAINLSGGYQHASLNRGGGFCIYPDITLVVNYLKKYCNLKKITIIDLDAHQGNGYERDFLNDSSVYIIDFYNSYIYPRDRQAEKAIKCFKHIDKRTTDEQYIEILQRDLEVNLKDDMQFIIYNAGTDIMAGDPLGNCSISASGIKKRDELVFKCARHKKIPILMLLSGGYQKENTFAIGESILQLIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YGG5</accession>
    <name>A0A8S1YGG5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPENT_87.1.T1680043</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000168">
        <property type="protein sequence ID" value="CAD8212438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YGG5"/>
    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000657">
        <property type="entry name" value="Probable ATP-dependent RNA helicase DDX41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="16">
        <location>
            <begin position="111"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="142"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="337"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="533"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="11">
        <location>
            <begin position="111"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="551"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8212438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="560" mass="64277" checksum="1721F9C58B14C4E5" modified="2022-10-12" version="1">MHQQQQQQDEQKRSLVQEKMQMKQQGIDNGNMRKINDEDIQKIQAYEQMQIENAMKNSTWSTLMLLKSKEIVKSRWRPKKKQRLWDQYKIDKILKKYSIMIEGNDPPPPIKSFQDLRVDDRILKILSKMKIKKPTPIQMQGLPAVLMGRDIIGVAPSGQGKTLVFLLPALLQCVDEQMKMPIIRGEGPFALILLPSHELAILTYELAKQYCHKFQKKGFPEIHCLLGIGGMDMSSQLQSIKNGVHIVIGTPGRISDMVNKKKINMDLCRFIVLDEADRMLDQVFELEIRNILEHFTGPRQTMLFSATLPKKIQEFTKQTLVDPLVINVGRSGQINLNVIQEILYVKQEEKLHYLLDSLKKTTPPVVIFSEHQNDVDDINEYLLIKGVEVVGLHGGKQQEDRTKALKQFLNGQKDVLVATDVAAKGLDFPDIKHVINYDMPKDIESYIHRIGRTGRQGKTGRATTFVNRQQEESILLDLKYLLVESKQKIPQFLEKLKSDEDLNGSCGYCDGMGHRMANCPKLEKQKMRILTNPNKESCPDFLKGQNYRESQSAERENQMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RUL4</accession>
    <name>A0A8S1RUL4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0010230</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RUL4"/>
    <dbReference type="OrthoDB" id="290386at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10527">
        <property type="entry name" value="SET_LSMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1410.10:FF:000038">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF153">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="251"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="282"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8131676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="305" mass="36324" checksum="88E1CE2C9DED9D60" modified="2022-10-12" version="1">MFNSQKTQELCNQEKYIHFIKWCKENGALISDEVQYPSAFGAQGYTGISAKMNIAANKVIIAIPNKLIISHHKVLKSELSDMFKAHKPFFDDQITADAEFNCLALYIFYHKLQGEKSFWYPYLNVVEQHTMFEWRNRDLFNLQDQSLIDEFMYIQSEMDKSWYKFKGVMNKYPQYFGCLTEEHKDMFYWSNEFVMTRCFGWTLPSTSLVPMADMLNHSNINPATYHLINRLIEQNGQPNNRYTLKRERIDLHLHPLLSTYLVLFNLYIIKIIKRLFQLIRKCIFLSFLNYLIFILNQQTIQFLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RUZ9</accession>
    <name>A0A8S1RUZ9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0010290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RUZ9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8131796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="153" mass="18892" checksum="4E04E4D58F8150D8" modified="2022-10-12" version="1">MLSQIVQYLGFVRILYQKQELHLSYKENLQTIKEKIQNLYSNQKEVAFWKTFRQSDNQYSQRTLKHLLFKFILYIFLIISFFCSILYFDFLRFIQVFKKYSIFRKCRVYLLRSPYILINIYRDLMLNDTKQNEEIKIKINFNYLTETQLNKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RVY2</accession>
    <name>A0A8S1RVY2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0010506</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RVY2"/>
    <dbReference type="OrthoDB" id="288673at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8132228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="435" mass="52206" checksum="04A4D90B6018898A" modified="2022-10-12" version="1">MENLHPAEEIVRNILDNIEQHHKFIDFVNANSNKQKQGIWIKQYHKGEEIKPITENNIRIVELQNEYIPIKQQFHHIMVHKQFVPNLCGYHATYNLIQCVQSIKYKISPQFYDIAAFWSYVRRTQDFLKQYRDQRGLDGSVWPWRNEDIEKGDFERTYLKCCLESKPLFKQTFSNEVFDGIKYIITNDTIFYQYGNIVNGYNERLSLQKKFNLFQEFRPTQNDEMIQTYMLGVTNHWISFVAIKNMKGTQFIVMDSRNRDFFLWNQQQIKEFLQQDQLDRPKRGQKPLNQFYLDLYEQGMKDLQQLITLLISWLTGQSKLEAYVSNQKILVFLNPLIELLEISQEEYINLKFTNEKADELYQILALWSDQYRITVREYIGNVTQITQLNKILFLKALELAKCALNYQTKRGLWNQQKQSSLHRMLEYLQLVYKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S1N1</accession>
    <name>A0A8S1S1N1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0030163</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S1N1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8134158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="229" mass="26821" checksum="C1BED9F793BFCF7F" modified="2022-10-12" version="1">MSNIHYNFRFCILQVPIGHPIQQQVLKAGYQQYELEHQQTQEPFSAKQVQLETHCAILGQSVQQVDPCIQAPGEHFEHDVEQAFWHSLQTEAQLSQKQQSFLSKQYVSEGFNSQHISLNQYCLNKQVTEQYVTITELPLIFSRPKVFIDYSHTSTHFQFKLSLNYGQHYLIHSLFFKYDLGLQLKHQFEFGPEHVLHLQLQLQQLNPSQYAPTSGISSQHLLFKEYQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S464</accession>
    <name>A0A8S1S464_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0020584</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="251"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="284"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="326"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="368"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="410"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="452"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1096"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1138"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1180"/>
            <end position="1221"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1222"/>
            <end position="1263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8133739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1363" mass="152344" checksum="5E2DB17807273429" modified="2022-10-12" version="1">MPKITLSILQDITQSVSKRNSILKQKIMDQTFQEKEVPADIFYQIKDVDEHIFGVILEIFRNEIVQDCIGFLSDYRNQKHLEQQIIQGVNLNQVEIQQKFSAFRNDIKIISDVLKKLKGHDFNNKDYSSEENYEFTLSLINSIKDDRQIIEFLLFLVRITSIDNTLIKCGSNSLHILVQMKVDIRDKNFQNIKIQNTSLVGANFVRCNLSGSEFYNINISGMNLNGALLLNCKWNNLRINELNKINTQACSVCFSPDGNILASGSHDHSIRLWDVKTGQQIAKLDGHSSIVYSVCFSPDGNKLVSGSGDKSILLWDVKTKKIIAKLDGHSSDVQAVCFSCDGQKLASGSRDKSIRLWDITKRQLKVKLNGHTSYIQSLCFSPDGNILVSGSGDKSIRLWNVRTKKIIAQLKGHSSDVQTVCFSPDGNTLVSGSQDKSIRLWDVKTGEKKAKLNGHTSTVYSVCFSPDGNTLASGSNDKSIRLWDVKTGLQKAKLDGHXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXHDDNSIRLWDVKTGQQKAKLNGHTCIVKSICFSSDGNTLASSSNDNSIRLWDIKAGQQKAQLDGHINLVISVCFSPDGNTLASGGYHNSIRLWDVKTGQQKTQLDSRTSTAWSVCFSPDGDTLASGSDENSIRLWDVKTGLQKAQLDGHTSTVYSVCFSSDGNTLVSGSNDYSIRLWDVKTGKQIQTTNTNYKDNLAQFTTSLLKNNHFLVSNYSILVVSQQIILEAQGANILGKIQENYLNLKEVDFWKLYSNNEFDYYQEHQNNNNFVIFKQDIHNETVNFSRNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S7G7</accession>
    <name>A0A8S1S7G7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0040346</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S7G7"/>
    <dbReference type="OrthoDB" id="307208at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="244"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8135675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="255" mass="29467" checksum="5C07D72C8343EE29" modified="2022-10-12" version="1">MKLPSINKPTSAQSANRQLPPKQNNNAISNLEKNLFNKLNEYDDINKIDTKKAQINEVKSNKKINNSNDEIDSIAKRANTLKNYSQTEKIKDLKSTQQKQPQKDTVFITHNQQAKSEESNNDQDDSNSDSSIEDLLQKSKVLDKQKKVMLNFEDDSEVGQLNKMLFQTDIDLDEQFQSMCILKKTISQMQDSMKSMGYGINEKLMDDLNQQYEEMIKLQRESQAIEEYQMKESNTKNKMPALRSNSVNQSKKQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S845</accession>
    <name>A0A8S1S845_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0050043</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S845"/>
    <dbReference type="OrthoDB" id="310866at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8136228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="274" mass="31748" checksum="099C3D5B2BA194F5" modified="2022-10-12" version="1">MQSNNPKLGLISNQSNQKYTDTQQENDVFELQDFGDDSDQIQFDDTICQNKAIVLANLDQEPITMVTSNNRSNQSQQVNQKSQFQASYPIKQQVPQVFIIWLYVYSIKCKILIIKQIKIVLGLFLKKKENKVIPIKFLKFKMKIQTQSTCMFCQIPQMIPKNKNVFICYNCKQINKLIFAYFICGTCRLTVMYQQGISNLINCTNCMSINYVQQPNLPNTFSQVKFPTIQVQYLVNQTQTISTISNQQQGQGIQQQQQRNQVSDLEAEFKELTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S865</accession>
    <name>A0A8S1S865_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0040370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S865"/>
    <dbReference type="OrthoDB" id="1739076at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8135707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="173" mass="20753" checksum="9EA7CCE1909BCB9C" modified="2022-10-12" version="1">MINRCKYLFICYAITSGIAKRTIICRIGIKDFQILSYLNIIDYLKVNYIQKKYSNIFKLQQQKIRKLKIGFNNQQLASQQKNRKDNTLKNNIYKNTDILMFQVGGTIGDYEFTCYYKVDRQMNREMISILILQIKFTYLLTLNNNRTRIKDIRADLIIFIYLQLNCKFHKETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SB16</accession>
    <name>A0A8S1SB16_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SF-assemblin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0060075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SB16"/>
    <dbReference type="OrthoDB" id="307099at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038835">
        <property type="entry name" value="Giardin_beta-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37027:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_148, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37027">
        <property type="entry name" value="KDE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="44"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="206"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8137406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="248" mass="28810" checksum="E09D3E5C4FE414C7" modified="2022-10-12" version="1">MNPNTPFNERFAKISEKLSSIQLHHDSSKAHRIDVICGRVSGVEERIQDTITSYNRKLHSLKDEIVRLQKQIEEENNAFETQFEQRVREVAAFESRITTKLEQEISIRKDGNLKLQGYLDEKVVYLKSDIQTESKIRQEQIENITTSLENDLPKLYDLVKTEGQDREDSDNGTLRRAGDEIKRLNEGLGNQKKLREESESAIFEMLKDLVSRVKSEIEEEKKLREESQENLLGLLEDACNKIYRAAKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SD52</accession>
    <name>A0A8S1SD52_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Orn/DAP/Arg decarboxylase 2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0060168</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Orn/Lys/Arg decarboxylase class-II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SD52"/>
    <dbReference type="OrthoDB" id="5034579at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004586">
        <property type="term" value="F:ornithine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033387">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022657">
        <property type="entry name" value="De-COase2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022644">
        <property type="entry name" value="De-COase2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002433">
        <property type="entry name" value="Orn_de-COase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11482">
        <property type="entry name" value="ARGININE/DIAMINOPIMELATE/ORNITHINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11482:SF6">
        <property type="entry name" value="ORNITHINE DECARBOXYLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02784">
        <property type="entry name" value="Orn_Arg_deC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00879">
        <property type="entry name" value="ODR_DC_2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Orn/DAP/Arg decarboxylase 2 N-terminal" evidence="6">
        <location>
            <begin position="146"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8137590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="259" mass="30849" checksum="6CD188C695463F46" modified="2022-10-12" version="1">MNLKIRQINGNYLFLGSHLIKQLRAILLNLYYKISQMDYMELLIVHQKEKSKWQLNMEFLIRSLFIVIQLKRKKTYTLQKIKEFQQQVLIVLRNQSRSKRQHHRQRYYGEYLLLKRTQNKKLLFFLVNLEMTCYIQILLIKDLSKFKKMKIQLHGIHFHCGCAVQGSSSFGKGIDLAQYFMRIGRLYGHKMELLDLGGGFPTGSIHGYAIKALKKHKIILQDMKQLLKQEDIFVLIHVHFCVQNFDQKNQAWQIMLSCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SE52</accession>
    <name>A0A8S1SE52_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0060420</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8138040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SE52"/>
    <dbReference type="OrthoDB" id="76265at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8138040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="370" mass="44276" checksum="F52DCB8D2DD50CCD" modified="2022-10-12" version="1">MSDQEILLVKIFRKYFLNAYKLIFSLDQKQADRFKEKNTYYYTQQHKDCHHEHKDDDRNCCGSFFAKVYIPEKERSALEQIVFEQLSKNNIRTKLPQILDYLQQRFNSIKKSGKKDNLQWDVETEVYILNEVLREGLQFYFLKEIRLEMQKEQYIDSKQQMLVATPQTWINKYPKIELNFLSADFMYDLMNTGWSIMNKFINNNDYCHALYKELDFLERDGRFEEVQTELQRDDKTFWLTLSQMDKDSFKNLYFISHILSALPYELNSKNKDLLVQISESYQISYFGGKEKKHKKHFDSSFDKKADTGKKFTFLYIINPVTIEIDEQKISLQPDNIVGLMSRKIPYALYSNDGRAFLIRYFIDGPVTNIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SGL2</accession>
    <name>A0A8S1SGL2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0080106</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SGL2"/>
    <dbReference type="OrthoDB" id="1517790at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042655">
        <property type="entry name" value="LRC72"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46759">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46759:SF1">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="271"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="211"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8139453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="432" mass="51136" checksum="9CFA5B64E7FFCC31" modified="2022-10-12" version="1">MDQVFNHLIKRATPQPIIKVGTPWQCIPIKNDKQARDTTEVHMSNQGFNELAQFDKFMNLEVVWLNENQLTNIKGIEQNFRIKHLYLQKNKISTLEGLQNLKHLQTLSLFNNELRDLEKNLLILKEFPGLSNLDLFNNPVAEEPYYRNRVISALPQLQLLDRSIITVQEKIKVEAWYKYFKAEVKIKKKGQKKQKLLPSQIFSVGEKILYKEVDQIKAKREQEKADEIQRERTRIKMKEKTFDPNKFPTNELKEQLRQRLHDNPMSIVNEWEKGKLKKIFKSYDKEKKGLITQDKLQGLFGDLINDIANIGKVPAVSFEKFKEIMSEDPLPWDSFCFQLNHVDFQRAPEELLQKKIDEKYKEFNRRLNAGKKAEAKEFMIEAVRLEAARDKDEHKLPEIIPDDNTKIRSDCFDFSRYKYKQNFTDTAKYLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJ27</accession>
    <name>A0A8S1SJ27_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">60S ribosomal protein L36</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0040406</name>
        <name evidence="5" type="ORF">PPENT_87.1.T0080068</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL36 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJ27"/>
    <dbReference type="OrthoDB" id="302801at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1760:FF:000001">
        <property type="entry name" value="60S ribosomal protein L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000509">
        <property type="entry name" value="Ribosomal_eL36"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10114">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01158">
        <property type="entry name" value="Ribosomal_L36e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8135756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8139377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="109" mass="12576" checksum="7E1241F26CA4D309" modified="2022-10-12" version="1">MGRTGIAIGLNRGFITSSLTKKQLRRRPSQRKGTLGKRVLQVRKIIQEVAGLSPYEKRIIELFKTNQPKDLKKAGKLAAKRLGTNRRGKRKIELVQNLYRAMRKQQQKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SNH6</accession>
    <name>A0A8S1SNH6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0110058</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SNH6"/>
    <dbReference type="OrthoDB" id="310609at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8142275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="256" mass="30829" checksum="8DCA93FC592B64DD" modified="2022-10-12" version="1">MQQITSLQCQEHFEKIQFLDLRQKILKGERAKCCKCQLSNPIYIEAAFEQWIQKKQNEIQQVQNDYLSYINPLKQFYEQMKQIKKSFYSSCEHIMNQIDQLINGLNFDKISRIQDLQLFDENITLSTLQEMAQLMSEKAPILKKSKTDINLLVKLRLLNDFIKQEMNQQNNFVIKILKQQQQAKEVIENFKEQYLQNIKKYEFKEIKNIYNIKQYEICRVVTFNKDDSITITFNLLIICNFKLNYRLNTLYLTFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SQH2</accession>
    <name>A0A8S1SQH2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0100463</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8142098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SQH2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="173"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8142098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="204" mass="24080" checksum="F32FDEC4691B9ECF" modified="2022-10-12" version="1">MNSILKVVQPYIEKQPKSTIKQDSGKQIQNKLHRRIKNPKTDFNTPIAIKQSLKQNEISQRLKKLLQISPYNKLVSQRSQQQLQYQDSFKSSQQQNCYTQNQQQSQLLFKPEVRTNSLNSTNSSSIKPRTWNRAKNLLQKHSFDFKNSKSFRDKDKQTISQIQTLLEKTSTVLQQFKEELQKNNVEKKDLSRKIENFEQINPNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SQL1</accession>
    <name>A0A8S1SQL1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0090248</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SQL1"/>
    <dbReference type="OrthoDB" id="305335at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035727419" evidence="2">
        <location>
            <begin position="19"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="197"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8140722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="429" mass="48934" checksum="6BA564E35F741E82" modified="2022-10-12" version="1">MKTFAIIALLLITTYSQSNEIDVVLKMLADLKNGTSKQLEQLESDWSATKYSKQDIVNDLMRSASTQRGECNRRDQEWANKERDIRITLSYISWLEKRIKENNERLSRLDINRCESNSNFINDIKNSKKTLNLIAFLRRAVKNAKETDLPTLLQSAQFIQLKSYLADDQDEPEITSDDNEPPHVYEDAEVITDDNQQETQEVDQTNQETTEETTDTQTEAQDHEIEQHQETGESDAPVPEHPFEAAEQATNEQLAKEAAAFNKIIEKGQKAPAESGSQKDYSKFTVAQQELLNFLDILETEVRGSFSQKQDNQVTSAMGYSDFKGLIIKENETFKGHLIAEDINLEKLQNQLITILQATAACKDRLKKIQNSIDLANEDLNSAEAHFKSVSETLNEELNTFDDVYRIYSSQVGSQNQQYKRDVQGKITN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SQS0</accession>
    <name>A0A8S1SQS0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0030073</name>
        <name evidence="2" type="ORF">PPENT_87.1.T0120047</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8133980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SQS0"/>
    <dbReference type="OrthoDB" id="282273at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8133980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8143115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="69" mass="8121" checksum="7BD61C2677812882" modified="2022-10-12" version="1">MAKQWFTTLPSNYFMRQSRKGGRREILFTTIALGLAHFGLFEPLRNSYYQRGVLPTWERSVVNGQPWTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SW70</accession>
    <name>A0A8S1SW70_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0130175</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5800476at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000596">
        <property type="entry name" value="CK1 family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="11"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8144330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="301" mass="35364" checksum="8731D7FE93C59144" modified="2022-10-12" version="1" fragment="single">MELTFAQKYRFTLDKRIGLGAFGQIYLGNNIKTGEEMAIKLENVSNKHSQLLHEAKIYRILLLDGPVPGFPNLHWFGQEGDFNVLVLDILGPSLEDLFNYHGRKFSLKSVLMLADQLLTRISYLHSKDFIHRDIKPENFLIGLNKKADNIYMIDFGLTKRYRYQRTETHIQYGEQNQLVGTCRFSSINAHLGIEQSRRDDLESIGYMLIYFLRGSLPWSGIKTETKIAKYEKVGECKIATPIDKLCEGFPEEFVKYFNYIKSLKFEEIPDYVWIKRFFKDLFYKEGYIWDNVFDWAQETKX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SY18</accession>
    <name>A0A8S1SY18_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0130116</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SY18"/>
    <dbReference type="OrthoDB" id="765884at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052758">
        <property type="entry name" value="SRC_co-chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44200">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44200:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="16"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="50"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="243"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="281"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="370"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8144216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="541" mass="62018" checksum="AF3D1CD8EB300A8B" modified="2022-10-12" version="1">MEVEHNTGSDNLKQQAELHKNKGNDYFKRGLYSNAAEEYEKAIELCTNEPNYYGNRAACFLQMKKYNKCIKDCEKALSLDPNNIKFLRRKALSLQYMGLLTEAKPIFEQIVNLDNSEQSLKEHKLIKELIYNLQQTRQKLDANQYKEAIYFMEKVAKEIPDAVDILILYCECLARTGNICQAQEQLRLIQDKQGSRAEISYLKGLIELYGGNPDKAKSILQEGVRQDQNNKKCLVAYQMAKDQDNYKSKGNDCLNQNKFNEAIDYYTKALEVDSNNSKFNSIIYANRGLAYQKLKDHRKAVNDFDKSIELNEKYFKPYLRRGDSRQELGDLDGAQSDYQKVMELDQGSIQQMRQKINDITRKQKQLSKKDYYKILEVDKNATDADIKKAYRKLALQWHPDKNKENEEQKKLADKKFREIAEAYSVLSDKQKRQQYDMGVDPNDPMGGAEGFETNIDPTQIFKMFFGSEGGADFGFGNIGGGEYPGGFTTVFTTNLGGMGQNMRGGSGFPFQFGDFSQQGGSGFTGFSFPGMQFTQQQQRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SYI0</accession>
    <name>A0A8S1SYI0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Calpain catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0120271</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SYI0"/>
    <dbReference type="OrthoDB" id="445853at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00044">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.380:FF:000033">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022684">
        <property type="entry name" value="Calpain_cysteine_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022682">
        <property type="entry name" value="Calpain_domain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183">
        <property type="entry name" value="CALPAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183:SF379">
        <property type="entry name" value="CALPAIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01067">
        <property type="entry name" value="Calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Calpain catalytic" evidence="8">
        <location>
            <begin position="97"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="22"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="340"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR622684-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8143522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="920" mass="107248" checksum="F7F0F789308E5D14" modified="2022-10-12" version="1">MADNIEKNESIQSYMNYSEQRLMNEDSFLKQKQTQEEDPQAFEQSETHSQNKQIDPMLQQNDEEQEQEIQPIELNPLQIFESKKMEIIQNCQKNKNLYTDPDFPINNSSLYKDPQKPPEFAKDIKSVKWVRPHEISKDAKFVIDFEGDYKQGAFGESWFVGAVVIVGQMKKYNEKQNKQTQQLSQQQISQLEKLILDYDQFDDCGFVAFQFFKNGEWKQVIVDTLLPFDQDSKQILFTQCANPSEFWLPLMEKAYCKLHGNYETICDGHIGEGLVDLTGGIAEINSLRDPQLSKMIENDQLWQVLLQTHKLQFFMGCMNHNETKGTKNADTGTHGILENHHYGIVDVREFPKEKLRLMRIRNVWGPEGGWNGAFSDDSEEWDKHRNLREELKLVFKSKKSDGTWWMSYQDWHQHFNKFYICKVFPESWQQYAIQGNWFGKTLGGVCPLKMPSEGLPDYVQVDTDEKWFNNPQYKIKVHKETKLYLSLMLEDEKITQQPYVACNLMVIANKARLSRIWERPPNQDIVIDMNPKGDIQAQREITQYLILKNFEGKTFGNYMVVPNMIVNETRKEEKRNFILRIFSSDKIDVAEMAETLEIQQEGSWNETSAGGKRKYDNGKENPNWCKNPQYFLNLSVSTHLKIILRRQGQVKRAKGTKIGMTICRFDKAPSNSLNIIKQQKGGGTNLVRLLKQTQQQLEAPKMVGIQRKLLIGSNEKFKQSTYFNEDVAALYFHFNPTEGPFIIIPSLDQEDRSANYKLTIYSNQEVELTRLDETKNQVHIGKWEQDISDGGCHLYEDPYEKDTSKRTWTMNPKYSLQFFEPIHITITLCIAEKNWKSKTKNTVGGMIGVYLIEKSENKITTQQIVRVPNFLPINEIQEEFDLQPTKNGYYIMPTTYQSKIHGQFILAVQGDKEFTLQALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T1X4</accession>
    <name>A0A8S1T1X4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">nucleoside-diphosphate kinase</fullName>
            <ecNumber evidence="3">2.7.4.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0100439</name>
        <name evidence="10" type="ORF">PPENT_87.1.T0130408</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 7">Belongs to the NDK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.6" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8142050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T1X4"/>
    <dbReference type="OrthoDB" id="2162449at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04413">
        <property type="entry name" value="NDPk_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.141:FF:000002">
        <property type="entry name" value="Nucleoside diphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="entry name" value="Nucleoside diphosphate kinase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00451">
        <property type="entry name" value="NDP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001908">
        <property type="entry name" value="PRK00668.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11349">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="Nucleoside diphosphate kinase, NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51374">
        <property type="entry name" value="NDPK_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside diphosphate kinase-like" evidence="8">
        <location>
            <begin position="3"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8142050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8144802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="152" mass="17832" checksum="FC2CA5499DE640B9" modified="2022-10-12" version="1">MEKEQTFVLIKPDGVQRRIIGRIIQRFEDKGFSMVAMKMLIPNQELLQKHYEELMFKPFFPCLICYMLSGPVIAMIWQGKDVVRQIRSMLGEGRNPIEQKPGTIRADYALELQRNLIHGSDSCESAQKEINLWFNQKEIVDWKPSDLNWVCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T4D4</accession>
    <name>A0A8S1T4D4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0160299</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T4D4"/>
    <dbReference type="OrthoDB" id="303213at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8147280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="251" mass="29765" checksum="426FE8EDA85B1550" modified="2022-10-12" version="1">MGCGGGKPQNKNPQAHQQPPNNIFEEPTISNENNTPNLKLEYILSQKPLEYLEKKRPNYDNLINKLVTEMNIQKMEFLSYNQLLVENYRKINKEHNLGKELIESSINFMNQELEQFIKLEQSMDEQDQLLQQKSFKFLIELYLILQTMKTEIQSECYWLGHYMNKYYDQDHQITEQNDNPNAQISNLKSIELQLKQGIQKAIQKIQHRQRQQQLMKINNSQDEVMSKINQNQSGTLVHYARSQNQKYKTPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T723</accession>
    <name>A0A8S1T723_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0160276</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T723"/>
    <dbReference type="OrthoDB" id="10249433at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF197">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_38, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8147236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="258" mass="29837" checksum="3BCF3DD621136F5A" modified="2022-10-12" version="1">MLSLNKLIYLNPQPSYHSKTFDGHQKLKLLFIRTKQNKHQIPCLFIKANSDEYLLYFHSNAEDIGTCYEFTSALSNGLNLNVICMEYPGYGIYTQVEPSQQQIEKDAEDVFIYINLELRVPDSKLTIFGRSIGTGPACFLASIYQPKALILLSPFTSIKAVAKKHFYFAANLLKDQFDNVKRANKIICPCIIIHGKLDKFIPIQMAEDIYKSLASKRKTFFYPEDKDHNNFNFSYDINDIVYNFIKEIEQLNHMFSPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T8Y4</accession>
    <name>A0A8S1T8Y4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0170032</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T8Y4"/>
    <dbReference type="OrthoDB" id="283742at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001129">
        <property type="entry name" value="ras-related protein Rab-44 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8147574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="342" mass="39222" checksum="44057ED5AB1EEF16" modified="2022-10-12" version="1">MFACCGKKKKERKHASLGNLHQLNLICQDGLYFDKNLKIITEFNKTNYFSFVIPYKETANIIVQSVLNIMTVCFIQLVVQIMFVMFVQYNLSPIKCIIVIIVEMITANMSMLILHISQKYTQIHILNYEIYINQYIQMQKVDYIFKIVLIGSQNVGKSSIMARFIDQVFNDSYLSTVGVDFRIKTLSIHDKTIKMQIWDTAGQERFQALTQSHYKGAHGCIAVYDVTCERSFEDAKKFLKLVIEEHGLIPEACYLIANKVDLISKRVILSKNGSELAQDLGVNYLETSAKTGDNVNQLFLDLGKIILNLVDQKRTNAQPPENDTRLRTLDTQKIEQEQGCKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TA30</accession>
    <name>A0A8S1TA30_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cilia- and flagella-associated protein 300</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0190010</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CFAP300 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TA30"/>
    <dbReference type="OrthoDB" id="10259249at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029416">
        <property type="entry name" value="CFAP300"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31078">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31078:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14926">
        <property type="entry name" value="CFAP300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8149240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="168" mass="20097" checksum="5318B8C591391745" modified="2022-10-12" version="1">MLSQIKFQALTCKSIKLDLFDKLTEDRIVVKGHIKQCFEEQFENIQIADELRKALIMEDSEQYCVFNEADRQELLFRLFQILVLGGQLCQYEDDIQAYLDWTKYLYKNTVNARKYADKDEIYIDSYAYEIKKLENSYNSDHPQNVMYVVVNPSLRIVNIIENQWLKVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TD31</accession>
    <name>A0A8S1TD31_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Parkin co-regulated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0190102</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TD31"/>
    <dbReference type="OrthoDB" id="300918at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019399">
        <property type="entry name" value="Parkin_co-regulated_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21207:SF2">
        <property type="entry name" value="PARKIN COREGULATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21207">
        <property type="entry name" value="PARKIN COREGULATED GENE PROTEIN PARK2 COREGULATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10274">
        <property type="entry name" value="ParcG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8149424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="305" mass="35099" checksum="6AB651AC4245999C" modified="2022-10-12" version="1">MLQQQQTNKRIKGHSPQRSPGKFCSNLLNISNKFAGTGGMCSSLAPKKSQAPQKVPKSGIFQALPMIEQTLFKKYYDRGDLPIAVNFSGAVRKIVWKCEPECLDYHLYLPIFFEGLREVEDPYKFLAVNGCEELLQKGETKILSVLPQLIIPIKKALATKNHDIMCITLKKIQKLVKSGQMIGEALVPYYRQILPVMNMYKNKRLNIGDKIDYSQRKNENLSDLIQETLETLEKNGGEDAYINIKYMIPTYESFELNSLQLKISIIFQKLLKYHVNLKRVKFSSEAQRKVKQFISLSMLNYYQKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TE04</accession>
    <name>A0A8S1TE04_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Calcium-dependent protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PPENT_87.1.T0190113</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Plays a fundamental role in microtubule organizing center structure and function. Component of the infraciliary lattice (ICL) and the ciliary basal bodies.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the centrin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TE04"/>
    <dbReference type="OrthoDB" id="289693at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000726">
        <property type="entry name" value="Calcium-dependent protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000634">
        <property type="entry name" value="Calcium-dependent protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000178">
        <property type="entry name" value="Calmodulin-2 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="33"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="18">
        <location>
            <begin position="340"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="18">
        <location>
            <begin position="420"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00025692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAD8149446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="509" mass="59618" checksum="4B6B165B37E8E2AB" modified="2022-10-12" version="1">MGICSGKQQKIRQGKHQLSEIKRFAKQNIRQFYRFGKVLGSGSFGTVKVGYSVRDSKAFAIKTIHKYRMREQFYLVLRELDILTQLDHPNIIKVFEEYEDDMYYHFVMEYCSGGELLERIVEQGNIGESESKEIMQQLFSSINYLHSMGIAHRDLKPENVLFASKEKDAPIKVIDFGLSKKFRNINQKIQRMNSKVGTPIYVAPEIIAGDYSFQCDEWSLGCIMHVLLCGNPPFQAKKLDMLETKIKHSDIDFSFSVFEHISQEAKDLIKNLLIKQPKKRITCEQALEHQWFKNMKQSSHQQKLSNNQHQKIIRLLHTYANSSKLKKETLKILINQLTESQISQLKDVFVSYDKDCNGTISIQELLQIMSNLGFKESEEELVQMIKKFNNIHPFNDDITQDTQITYTSFLSALVNCKSYLNKERLWNLFKYFDSDNKNFITIEDVRKAFEREGRQLTQSKLVSLFSEILQNEEQKIIDFEQFCQMMQDNLYNSNNDSKNLEKELDCFEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TGE1</accession>
    <name>A0A8S1TGE1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calmodulin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0200096</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TGE1"/>
    <dbReference type="OrthoDB" id="288000at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="6"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="116"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8150112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="184" mass="21748" checksum="24481E03E24A49C3" modified="2022-10-12" version="1">MQQQSANQQELKQIFQLFSQENSDEINIQQMNQLIKELETINQDQQQISVPYQHGRKLVKKISSTDNFEDMQKDSSPKSPTYVSVTELKYFPNNKTTMHFSDFQELYNECMSNKDQHDELLESAFSVFDFDKNGTIDSKKIRKVFSKFKDNTSEEEIQNVLKFCGVLHDQMSLQEFKEFFKKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TUJ5</accession>
    <name>A0A8S1TUJ5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0280050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8155940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TUJ5"/>
    <dbReference type="OrthoDB" id="284661at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF197">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_38, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8155940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="307" mass="35942" checksum="64856671E0B68E00" modified="2022-10-12" version="1">MNPSDTVAICFFSLVILLYLGLLVYLHCFKKIYRLIFPSPSPMYTEKFFGDKLKYANVYNSLVNLKSDQLLNSYNLERVRAIPYVYLKNKYSETNNYLIYFHGNAEDMQAASQFMEHLMKTINTNIFVIEYPGYGVYEMQKNMSSNLIEEDALTLYDHIKKTQKLLDNQIYVFGRSIGTGPAFYVASVRNVKGLIVMSCYKSIKTIIQDLCCGFGIIIVFFFCLPDIFKNYERSQQVKCPIVFIHGLHDTLIKPYHSNYLYTNLPRSIQTKSQIFIKERMTHNDYDIDNDIACSILELFQELRYQKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TUV8</accession>
    <name>A0A8S1TUV8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0260148</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8154799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TUV8"/>
    <dbReference type="OrthoDB" id="302992at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8154799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="347" mass="41017" checksum="9AF4D3281C660CB8" modified="2022-10-12" version="1">MKRKHTLLLRKVVAEQNQMSWPFADQDLHSSNIFQTQSPDYQILDFSHKNSTNEILSRSIVGKVDTFNKQANDLEKPIRFSQPVKLSNMQKRIKRTNTNEPSSLNSSYRSKKNITNQQHVIQKTGDANFEVLDKYELIQTMEKIQKIIKNNEKYAQDQLQKISATEKILKTQHQRALNKHEKQLQVWNELSTKISTRMKNNSNTLIERQAQYRSRLDSLEMFEELKPEESKNPVITWHQRLRSQYRPQKVIQKEEQERLQLKQDLLQQIPSRELLEIPNPQKLISDIKNKNGYDKGRSVSDYNGLKLVGLSVYDQELNSLKKDQSQIQQQYYYYKVPKEIKIGEENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TWC3</accession>
    <name>A0A8S1TWC3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0280001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8155842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="303797at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="235"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1010"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1223"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1339"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1585"/>
            <end position="1606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="923"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="933"/>
            <end position="946"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8155842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1650" mass="195697" checksum="4AE0AA5687ABAAF5" modified="2022-10-12" version="1">MQFISKLSSYFVAIGTNIERRTKNIDYGNMAKILYYFIQLIGYIYSQFERKAIQYYENDFLAQVSQQSSIPFILIIIHYDPLTIFAYFCVFIVMLSTFVIVLFQILLKSLPSSSPINKFVKFYFQNFQWFFLTPFNECMIGVITCGKQSYLIQHSVIEPSECLQQISPHYVIISFLGVILVSLSGIVSVYYFRNYDFLQAGLLRKFSHINFITIFLHQLLIILSFWKQMYQNEYVIVYSCYFLIMFCLLFDVFTQLPFGFTTETIFYSQILITSIFFGALISYWMFSKSDDGVIFLTSCIIIPLIFTLNQAYFQNQFDEESLRFCSNIGQTLNEFTLEYFVLISHPITMTKQSYFKFIRYLNIHCQTCNDINCPCKKQLRTFITSNQTLDCEPLYVWVQYQFQKLIKLSMNKECPDGIFEQLTIKFVTFLKKYRSNQYLSFKNIQDVVISYKIQQKNQTNSLQFFIHLTRQIQLNSKLDIEQQNRNQHLLSKNEYKSLNDLNLFLFYEIQIMQNIMMALYLQKELWTCYTNGNIKNYDDLMVQLKKISIQDSIVQKDYNYFNSQREDYKYENILIIRLQLLISMVCLDDLNKQLSLAKKLQLMQQEQLAQSSHQFHILNYINGVAITIQQLVSFEEYDIKNISIKDNCKKFFGYENSDDTPKLKYLIPNKIRSIHKGLIDRFLFFGHSSKIYSSQDTFIVNKNQFLEKVSMSLTVLFPNQGNDYYFYVFAHLLKKSGSQKNNQIMNQQGYILIDKNFNIFGMSQSIQNKINQFSQNQINFCEIYDKLSIFYIFPELLVKLQEHFTDKQIRNIRQQDEEVIIQKNTMNFVLPNYDIIINASNQKIKNNKIDFDIKNRHILNDLIEFEKQFTEYQNTSYYFQVEFTMISKILNPLIDGIQKDFLYFLVDIQFENDQKSSKCTFQRLPSESDENQQKNTSQQKIHNQTQNQTQLVIQSFESENREFLSYKQIRASSTTSEKRSSNNLNFQITFLRQMITNSILPNQISNLQSFFLLQIILIIGIIISLLIFFDRKSSMQSDCIKIITLDLDFLDAYSQIMSGSRHLIYYRDFYTQMNDSTLLIDNKVLNFSKYNRLYIAWNHLNLGLNRLISIYEQNSKVFQQKSEESINIYIINGNLQNKIIQPVSELTTYFQIMFQIFYLSQQTQSQNISNYLSDSIDSYSTQISRSLVYYNYFDVVKIFQMKIDSCKNYNNYINEYLDSISNYVFVGFYIIAILIALSQLIFNYKVNKQIKFNFDIFVKCDSSDGNLFIRKNDQLKHILTSKSVLLRKQSFMRLLEKPFADYDQIKVQNQNSMSAIVKTFNSQDKHIVIKNHLSKFNQIVTLTFICVIGLSYGMGFQIYYNIISNNVQPYNEEAIDSQQLRLTSITTINKYDQYLIKTLFESYYNLALNDSSFIVTENILSDHLILNQLEFNTTLLSQEITEFQHLNFTNFLFNSKTFKTISKNEQNEILLHDTCVLTGCDMYSELLKQRMFNEILNPFFYQGIVNLHYILSQIYYEIDISLQYPQSNLDRFKKIQNFFNEHNYMIYIMWGLDAIQFQIGQFCQYFVNVSLDAIFNLTINSQQSIIIFGLVTIFAIIILQLLFIYYQIQRYHLSKSLLKLIPLEIIFKKNLYKQLVLHEKRSRVDLTFQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TWW1</accession>
    <name>A0A8S1TWW1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0290129</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TWW1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8156708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="111" mass="12568" checksum="A83486652851B6C5" modified="2022-10-12" version="1">MKGLGVFLIGIYIAIYGIALLTDPWTQLHYDRSIRQLGMKNLQQFVALALKVRSLYGFAHIFAGYCLMNKISSMKLIISCIFAIDIIIQIGFQSIHQIFQDVILIGIVNYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U0J3</accession>
    <name>A0A8S1U0J3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0320130</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8158735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U0J3"/>
    <dbReference type="OrthoDB" id="284724at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8158735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="513" mass="61474" checksum="1830B96CBB6FC5EA" modified="2022-10-12" version="1">MYNKKFFQLRQLTRNLDDCYHNDLEIYNQIMGVVLKPNEKKLENDKQLLKDWDVYKQRRLENIINETLKSPCFKICICKFVRNRKTSIEFWIRVAYDVFYDDSLGKEIRKKFWSKMKEQSPNLESDLQQQQWPDEWKYLEYESCLNLQLIKLYEQYSAIRLFIAQKVNQYTQIQCDIGRMPLLREEKKIRIDFSKSTWFPPIEINGPDLLEQIFISDNYYCEQMARFIVRMKDIIFPILDFFKKENENGKTINIYGKKYEFILDVVYSLKACCGPKINFTSGSLRMRKNFKCSNSYFVNEATCSVMLTSLQKEDITKDVIGDIHLNSQVGYSAFVQTAYELQEIGQNERMKRLVIIFSQEKLNFKEVKKKVIYYQLPDKDIENLINSLDYEFLQYYANLLFNKCMIVQQNCSSRKPFYQIMICMWTYVLCIYFQDELPNLVDNEVFNYVNQKLQQEFEIDLGLSPERENQKTIELDKPQIIYHEIGEPITVESDSKEDDDCIIIDKPQKYTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U1W0</accession>
    <name>A0A8S1U1W0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0290113</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U1W0"/>
    <dbReference type="OrthoDB" id="286314at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8156676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="191" mass="22665" checksum="6C22F8229BB7D6AD" modified="2022-10-12" version="1">MQQRQRKISAIFKRVKERSVQMAVEKVEEWRNLFQNGKLDNQGNLKKLSLQQAAEEVGIPKKTLEDYYQLIKKAKEIQPIEQLYDHKMGYLRQLIKQSQNFKQNVIEIESEDQQQVDSDSDNEIQYPIEQTNFDYEEYFNLGNDYNLDQNSVVMTVHTRPQIIQIHSKKVDPWENETLSCDSGQETDDEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U291</accession>
    <name>A0A8S1U291_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0320071</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8158648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U291"/>
    <dbReference type="OrthoDB" id="291456at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8158648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="64" mass="7403" checksum="1022244B4F81F06E" modified="2022-10-12" version="1">MGICKSKSKSSIDVQYQGSFAQIINKGSEKNDIEAFIREKTQSQDLFNPYRNPILNRRLRQTPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U6E1</accession>
    <name>A0A8S1U6E1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0320219</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8158909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U6E1"/>
    <dbReference type="OrthoDB" id="291611at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8158909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="201" mass="23419" checksum="D8F121C980C1CC8F" modified="2022-10-12" version="1">MRQLSSPLTTLQDNLQSRSMPRIHSKQEQRFQLPTLKSKINNLIESEEQKAPVGQFFQGLVKGKTIMESKNKIEFPKTQQNFYRLVYKAQYSFRPTKLNPRQVSMDQPAIQKKLGGPTFAYRTIQSSEEQLRKPNLEVIARQQVNYLNESHSQNESLNTEINQILYGKGQKKMILKQRLNQTKNNNDTSNIIKEESENSFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U6K6</accession>
    <name>A0A8S1U6K6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0340317</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8160400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U6K6"/>
    <dbReference type="OrthoDB" id="10069349at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23804">
        <property type="entry name" value="UBCc_UBE2S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000031">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="UBC core" evidence="10">
        <location>
            <begin position="50"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="205"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="226"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8160400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="239" mass="27934" checksum="F70A7203BD84C67B" modified="2022-10-12" version="1">MNEIFIHFYVAFIQFQAYLLTIQRIQFKIKFIKLTYQVIRMIQEPQINPQLGPIIAKQLQIMIKQPIDGVHVIFNEQDVFDIQADIEGPVDTPFQGGVFRCKLILPPQFPQVAPKGLFNTKIFHPNVSEKGEICVNTLKKDWNPLQWSLKNIFEVIKCLLIVPFPESSLNEEAGKFFMENYDEYFKRAKLLTSIYAIKQDGNVLKGNNQNTNDEADKKNKLTQTQQKKENDQKKWLKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U6T9</accession>
    <name>A0A8S1U6T9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0310293</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dpy-30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U6T9"/>
    <dbReference type="OrthoDB" id="417678at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd22965">
        <property type="entry name" value="DD_DPY30_SDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007858">
        <property type="entry name" value="Dpy-30_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049629">
        <property type="entry name" value="DPY30_SDC1_DD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05186">
        <property type="entry name" value="Dpy-30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8158346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="113" mass="13131" checksum="BE7AAC2F50237F46" modified="2022-10-12" version="1">MSNKRLSNSSQQQQQQKKPNFNDQPTVIPTKEDDKKSLNGSQINDSQDGNQKQQQSKFDLPQIPKFQSLPLRDYFDNKLSAILLEGLKEVGRQRPENPIRFLGEYLLEQDKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U8Y9</accession>
    <name>A0A8S1U8Y9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0360111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U8Y9"/>
    <dbReference type="OrthoDB" id="288779at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8161320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="126" mass="15413" checksum="411D13BD01465A5A" modified="2022-10-12" version="1">MRRQKQFEFNINEKISYPYTPLRISPSFRIKFQSPTKVQKIKFIYQKDLKEYESHYFDQFVDTHRIPTIHTQRAASQKQPQMQLRQQTPNYINLNPKDSEQENDKLSEFLRIIRGKKQVRINEHQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U9I8</accession>
    <name>A0A8S1U9I8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0350029</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U9I8"/>
    <dbReference type="OrthoDB" id="288440at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016650">
        <property type="entry name" value="eIF3e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10317">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="4">
        <location>
            <begin position="183"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8160523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="386" mass="44749" checksum="42BEBCF4A570CB55" modified="2022-10-12" version="1">MDHFQRFINPHVFLGCVQFLQLQTSKETEALLGTRLFQRLIKLGVPQEKIDQIIQQDNLNGKLTSVAELLKLRQRALVDYIQENLWTVEEAYQALIILFDQGEYEKATEIVDNLYPCVEATEKLNHLRNGFLWARLNCKLVGLFKNTNNAAVCIEAIKDLLKQEDQIKYNFKQRADLLHAGVLICFISDDTNAFLELFSGEQFLEVVHTIAPYLLYYYTVALLINQQFIGNTSLAIIGTKTNKQLKQYHLLNYVEEIIVKFQFDNAIKMIQDISAEIDQDFIIKSKKALIINACKLYFFSTYIKIFNGVQIKQFSTYLQLNEEETESWLVNAIRTLNINAKIDGDKIIVQKQDINAQNTQLLRQLRDLQPKSNMLISNLQRIINIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UA72</accession>
    <name>A0A8S1UA72_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2 NT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0350072</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UA72"/>
    <dbReference type="OrthoDB" id="10532121at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019448">
        <property type="entry name" value="NT-C2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10358">
        <property type="entry name" value="NT-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51840">
        <property type="entry name" value="C2_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 NT-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="229"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="518"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="625"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="781"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="877"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1090"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1160"/>
            <end position="1201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8160609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1257" mass="150085" checksum="1DCE32C454AF63CD" modified="2022-10-12" version="1">MNPKGYKFEVHIQIEKMEMNSSLSFQVQIILKCGNQKVQTQQLADFNQGRAIINETLLIQTNVYQINGIYQEKKIELLILLITTKGKKKAGQCYINIAELLNQDKYDINQELFLENCPDNKAKIYSKIQLTKIGKVDFEAINTQYSIVNEQSSDKKQGIFTPFKAALENTAKKYFGDNQSAQSQKLENSFSRNQIKNPMVSDVSYQLNSQKQQQNLQLSNQVQQDLQHLHEELNQQKILQMEMKDQNNQLKQEIQLEKVKKETLILQIQEQKQQMEKMIDQYIYQEQIYQYEQQLSQIQCQLRQAQKDNENLTLNYKQAMQQYNKLNEEYDQYQQNKTKELKDISNQKDLLYQALEKSNNNNCQPKNINYENQIIQLNQQLLQLKNKIDEKDNFIKSQTQNINSLNEQLLNLYNQNSFLYYNEKMLNDKIDEQSKQILNQQEQINQQITVIKQQYEDLLYQNNQTIKQLKNELIEKNLEITSYQTICQQLQQKDQLEINEIEKLKQGGQTQTDIDLKYREKEQKIQDELQKYREREEELRTKVQFLMQQTNQFQTEVMRKLFLDPQIPGTPVNLPNQLIELNRAISIKFSKLKQDLECHQFEIQSQENINVDNSQNQQVVILKQLQLKTFELTQAKLKIDNLQNDNIYLQKQLVTKSEFLNQLNQQILNQNQKIQDLEIQNKDIIQKSILEAQIQQQLSEQIIIFDNQNQELKIQLKTSQIEIQQKDDLIKNLQSNLQQLNQQNQELIIINEQLQYQLKEQIHNTQQLQYNDLILELNQKLKDLEIFNIQLQIQKQEQENQIEKLQKDLFQQKEYQIEKQKLISFPTDYEIVDQQQKEQHHDKLTNLQGHLLIEESQKSQEKQLILEQKIEQLTIQLNQQTSTLLQAQITIDQIKNEYNQTLERAQLKETEFKLQLGQSQVLNDNLQTELDSQRIQLKNLEEQIQQKNQIIEELETQIKKIQQKLQIDIQDLQQQLFTENDEWQKEKEIIQQYIDELEHRALQLTNQVKEKDNKLQDLQIQNEQLNEQLIISQYNNERVQQLEQQCFDNIQKYELQLQQQSQDNQVKLLDQKTQLNKQEIKNEIEKQLIINNYVNQLRDKDDLIQHQQQEIQNYQQVKLEQQNVIKFLESIKDNFEQKQSENFEYIKNIQRTYEQIKVQSQQQEFLINQLQNTIRELELKNQELEMEKINISNNYQQALIEAQSLNHTQDNSNEIIEKIQEEVILLKCRIGDMLNEAQEFGGAQLVDKLQQALGIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UBW5</accession>
    <name>A0A8S1UBW5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0380216</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UBW5"/>
    <dbReference type="OrthoDB" id="290678at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="133"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="13"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8162741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="247" mass="28815" checksum="01899DE5202F3517" modified="2022-10-12" version="1">MKKTPNQSSGPKFIKDVTQKSRSEHNQSFDASYSKNYAPTFSNQSRLKSYSQQANFLDDLEEEQYPNQINVAIQKRPFELALQNELQKMLSKIIAGQHLSIDDCQSIIRAIDECIQESLQTLRLKDAEVQNIKQTYELKIHQQENTILALENEVQELKHMITLNPNDSINFKLQQLEQENEMLKLQNQQAIKIAVQGRTKKQEKELYDAMNSSKSKQKAQVNNYSILLNQQYNKIYQQDKKIKSQLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UEP4</accession>
    <name>A0A8S1UEP4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0390175</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UEP4"/>
    <dbReference type="OrthoDB" id="3246549at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24124">
        <property type="entry name" value="ANKYRIN REPEAT FAMILY A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24124:SF14">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_25, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="295"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="675"/>
            <end position="706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8163348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="766" mass="89548" checksum="E0D5911DC75BEBB3" modified="2022-10-12" version="1">MNETFEQCLSMQSGKNKSQRSPIQVKFVTQRSFQTNKQTNSDNEQQRLLLDYSRQGLIIFNDGMIWNGLLHLMLNENKLKEFDTNILKKTPLLQNLSLENNQLQIFGPIEFNDVPKLSTLNIARNLLKQIDPSICKLHLKKLYLQQNYFTNISKQIMDLSYTLEVLGLGWGIFCQSQFDFLLEDQKLKVFFTLVEAMSNELTFEQYIMNYKSISSAIIVTQGDILNDSINSQNEQLLTQIKEYLNYHSLEIETSLNYMNDYKQNLMHLAVVYDEIGILDYLLNHHLDLANQIDLKGHTPLSLAIEKKYYRLVSLILQFNINVNLGGGLQSSNLHIAAQQCQVELLKDLIDLGANPAQYDIFKDTPLHIVSSYSDHKRVKQFYQVILQQPFDVNHQNLKGQTCLEILIEKNNFFTLQFCSEWNTTQMRQFNDENLFNFCSINPHTKLSLLDKSILTKSFMIQQFLIDFQLVPITYQTLMLCFLPKIRNVFLYKILKKQQLIQIRNSLILQNSNLNEEVDSIPNVSPPVKAIEKFNLSDENSSFDDDQMSNISDNAPGQVSENLKQLNSWIPKFQTIRNGNVNLKKSFYDILSNLTECIPDDEISLIHFNKDIINNGSKLVTKKNMDQIYTSRLEHKLSRGNKQNLKQHNYSPNNIRMIENRNSIFKYDNYHQKRQLAILQKQMRQIIINLQNHLRESNQQNEEIIGQYFRTQFQILDQFLTSKIGKELITIDKPLQTEIRTLRTLIEQDRFKPKTINQVIDLFDLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UN75</accession>
    <name>A0A8S1UN75_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0420073</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UN75"/>
    <dbReference type="OrthoDB" id="284854at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="370"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8165162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="708" mass="83363" checksum="D2EDE58B2ED1275D" modified="2022-10-12" version="1">MGAADSKPINKLFEHYTQINAHNNDFPECKILQHKRDPNQQLILRTINITDEAHFKKTIHQYQIRQKINHPNVLNLNNYFYEFEQQLCGQFYKVNLLFEYPQNNLERVQTINEIQLIEYLKQAISGLACMQRNQISHNTLQLRYLFLMNGIVKVSDPQYFQQNTNYVQILQNPNCLESIYLSPTLVNTIRTNNWWPKHNQYKSDLFTLGMLFLQLALNQVINDCYNYIQGKFIEDQLNIKLQKLRTRFGQQFCDFIQMMLIIQEDQRPDLIQMEQIINQQEISNKSYYQQQQTPHVIPKQIIPTQPIGLIDTINQPNQLQLEFAYNTQINNQSKRINQVQTISPIEYAHQYTIKITSSQRSSTPINKVSIQNQQIQSRDCSLPKQKTQSQNTSSRQINYEYQNKKTQLQTNNLNNTIITTDQSIISNKTILNLPPKQQINQKNSISKQINQQPELNILPIQVIQIQQDQYDTLSKFVPSEIYSKYSKFDENIDSQISEDINTQRQSNCDYSKILIDSTNLPQQIKNTLTKEFNLPCETSQITAEKVDAPEFVVEHYGNGSRYEGIKMNGMRHGQGRFYYQDGGLYDGEWKENKMHGEGTLYYGTGQPAYEGKWNEDQFHGFGTLYNEHPQMLEESFDYRDFDYVEDYWMKYSGNFQMDNKEGQGSLHLTNGERFVGWFERDLINGKGVFYRMDGKMMEGRWVDNKLVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UPR0</accession>
    <name>A0A8S1UPR0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0440101</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UPR0"/>
    <dbReference type="OrthoDB" id="448448at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18008">
        <property type="entry name" value="DEXDc_SHPRH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23143">
        <property type="entry name" value="RING-HC_CeBARD1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF22">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="537"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="970"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="1037"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="647"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="647"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="666"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="718"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8166344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1208" mass="143588" checksum="2FE2EE2196BF0EC8" modified="2022-10-12" version="1">MIYNYSKQEEDQLQPSNYIGKFLCKFQTDEGFAKRNNPKQKYKINILSIPLQKIKDLKFHQKRLFVIGGGTLQENRTVLCKVKKGDEEIYKVYNKYGYIEHVFDELWYFLINAGYVKLRVNFVDFVVENKTITLTEIQVFFTEQAINDNYELKILRYNRKNGLKKGMPKYEKYQQQQLFHQEEIKMEYQRFARLSLLLMFDFMNIQVETPALVKSDKQFKIFFTQYCYFHERYKSQPINQLMKSYHRPIIENQNYFNTYINNKHFYNKLFPQDLIKKLALQKQGGFKYISNNQPYKPKIEKIHPLFHIQFQRVAVAFYEAILSFIRRSMSSVVKQQQPTYSDYVLKTIFLSTVPLKNNYLNDELYQQQHQNFIDERENFTNNLNEDNGIELLSDEKIRIYEDSKNQILDYFYVDYVVQVDKTQKNHLNLLNQQQESYFEETKLQQKKYNEDILQQDLNNIFVINTQLQEYNTPKQMLSQLKQHQKQALYWMLLREGHISESNKDQKQKLSPLWQQIRLLNGDTLYVNTFTGKISKEFIPVQETKGGILADEMGLGKTIMALALILETHKKGQQTLIVVPKSVLLQWEKEIQTHSKPRSLQVLVYYKQQSRSQKIKLKDYDIILTTYAILASDYSIWTQINEIESEQSEQKQEQEQQEQEEQKQKQSKQQKQQQQQQQKQSQKQSQKIKQQNFQEEDQSFSSDSMSDESISYSISNTKISEEQKEEVKNQQKKKKLTKKQLEKAKKENNLFKQNYYRVILDEAHNIKTRSTLQTRSAISLQSQFRWCLTGTPMQNKHDDLFSLLQFLQVETFSEYFWWNTYVNKEENEDDQQRILAQILQPIILRRTKNSQQFEGLQQVVETIHWVELDQKEKMLYKKLLSGSQNLFKNYVKNTANQSYVHIFQIINKLRVACNHPQLALKDINLQQTPLEMVLEKIDKFFLEKTHNGNKITEEYKQNLIENIKNGSITECLICTKSQISVFSLSSCGHIYCKECFSETVIKLKNCPSCRTKLTIQDLIEVVVENENVFEELQSLQFGLSSKLEAVIKETKVIKQKKEKVLIFTQWIEMIGLLENLFKENGIIAYRITGQMTVDKREKIIKNFKEQQDVTALILSLRATSTGLNLTMASNVFLVDPWWNPAIEDQAIGRADRIGQQNQVKVVRFLCRNTIEQQINLLHQKKKFYIKRALSNNQQKEQEFEDFKFLLFQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UQY5</accession>
    <name>A0A8S1UQY5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tubulin-tyrosine ligase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0460196</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8167035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UQY5"/>
    <dbReference type="OrthoDB" id="202825at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_125, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069">
        <property type="entry name" value="TUBULIN TYROSINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8167035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="507" mass="59500" checksum="FA19A8A697A50F21" modified="2022-10-12" version="1">MFLQAMKFKDNRLEPIQLKYSPKEPRQHTPEGLSKIGLFNSTQFHRKQSIEKRKLSSQAEGKRPQSSMQRIPRTAFLKNTFATQYVFFCENGNNGALVRRILQGRGWFAEQQTSFNVNFIWKQSNKGFNYSTLTQKKVCINHLEHHHEISNKNKLHDNLKIHCQRINKNMDDFVPITFSINLDSMTLQWDLKKFVDFFIQIKKEGNQKNVWLLKPPDLNRGRGIQLFSDLRVFINQVEEFCKIRSGNQKTKSSSKGARGVTITYPDSNEKSGQAQISFTIDQSTSNDRIIVLQKYLETPLLYNGRKFDFRIWVLIDHTSKYYFFKEGYLRLASEHFDVNNLKSLYIHLTNNAIQKNHPGYGKYELGNQLSFQDLQHYLNQQRNTKVTSAGIVVKMKELIHQTIYCAGSKLRDNRKDFQFEIFGYDFMVDKNGHIWLIEINTNPCIEESSPLLQKLIPRMLNDAFRLTIDKIFPPFKTTQENFLPNLHEYAIPGYSDKDNLWDYMGQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1USI4</accession>
    <name>A0A8S1USI4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0470117</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000047">
        <property type="protein sequence ID" value="CAD8167434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1USI4"/>
    <dbReference type="OrthoDB" id="424834at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052187">
        <property type="entry name" value="MFSD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23512">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23512:SF3">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="191"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="313"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="343"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="377"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="412"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="32"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="457"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="466"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8167434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="515" mass="57767" checksum="03B234CE092CDFB6" modified="2022-10-12" version="1">MKVDDINKTILKESQMSNEEDKDNEQQQSKLKYIIMVLGCLLMFGNNYSFDNPQALQKQLTQDLDISISNYNLLYSAFSFPNIFLTLIGGLIIDFLGVRFGIILFSAIIVVAQTIVALGGAFKIFWIMLAGRIIFGCASENLVIAQAAIIGKWFRGKELSTAIGYIMTVPELASAANSFLTPIIYESYKGLTYPLFFSVILCIFSFVCAVGQFIIEEQEEEDENEDQKDDIERVSFKDIKNLNGTFWILVLICTLTLGSYTPFLDDANDFLQEKFEFTNVQAGKVLTIPYLMAAITSPFFGPYIDKVGKRRKIILITCILFTLTHFAFGIMPNGKHGQPNWFSVIPLMLLGSNYALYSCVLIPSIQYIVAQKVVGTAFGLLGMFESIALAFFPIIAGYIVEISENPEIGYSNVGFFFSGISFFGIIFTLSLYIFDKKGSIVLDFVNPEDPSELEKKMLRTTKSESSSDEDDDDESSDEDSDDDQYKMKKVCKSYASLKSKKLLTSPLLRSRSLQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UXZ6</accession>
    <name>A0A8S1UXZ6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0490278</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UXZ6"/>
    <dbReference type="OrthoDB" id="317805at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="697"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="803"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="898"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="975"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1029"/>
            <end position="1053"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8168762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1096" mass="128179" checksum="F4DF86DD72347013" modified="2022-10-12" version="1">MIAQKGFWGITKFEISIIECSIGCDSCNNNRCFNQELFFNQFTSLDFSFVDIEKRWQTQDITNIQIDECLGFKYLVSNGDHLIKYFDLDYHHAISFQVKLLIFNSQSTYIHIFIDDVFVTSIKYIQVKFVMDLCKHISMQEINLFQYEHFNTKLKLEIKVQLIQDKNYKHTFIGIRDFQFFLINRSLDDRCFDNNIDPFDGCFSNNYDCIQGCGFCIKGICYGCMVGWEFNEQIGICIPYCGDAIITYNEECDDGNENVYDGCHECKYSCHNNCKKCEYGKCLECLIEYELDFHKNQCIPKCLDGIILYQEICDDENRIQFDGCYKCQKSCQIECLFCSNNQCYLCQEGWQLQQYQCIQICGDGQLAILSNEQCDDLENSYCTNCKYQCDPFCVICDKFNNCDICQSPLINIDGKCLPVCGDNVVTPLFEQCDDGNEIPYDGCYQCEYQCSYGCIKCEKDNHCVQCQNSQFFLDTQFFQCKTLQSQIEIKPEVDENIYIQCSQNQAFINNQCINLCGNGMFDRNFEYCDDGNNNGGDGCSSQCFEEDSYKCINQDNQLSYCTFIQHPNFIIMLLSDKQNQTKIIDLSFSQEVYLQSKLNFEQIVEFIIIPYTQFGLSVVPLNNLTSQLTNPHYQISIQFYSSVINPILQIKIQQDSILNQYDLDLNTNFKEIPLGTPFVLSKITQEKVIQVIKMNDAMVYSTVSIAGILFLTGNYIVFFNLLDLLQTLSYIRYMQQKFPPHLRQFLETYTKISLQPILDSLKVNEIIANLNGSTLPNQKIQSSQSISNNVLNQFYLINAKGCYLSYILSILTYIICCLISSNKVSTWLGIWYKKNEENLKILHFILKFQQKIQLKCLNIKNYYFSLGFFQLFYSTLHQQLFSTLLQFPIYNFDSPFKIINSIAALGNLIFLIHILLKLLSITTSKIKDKQKWKYFFQDTKSEFWATNYKPFQIYRTVFYISIITKFIRYPEAQSVLLSMSSLFYLIYLILYRPIKSNFEQAKLLCREIILSINTGSFLIYSLQLNDEQYLFYGWLHISLFCFLMGFTLIIDILEQGQKVYYIRLKQIQTQEKINIQRYSENPLQKFVEVNNLDIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V140</accession>
    <name>A0A8S1V140_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">STEEP1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0490099</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STEEP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V140"/>
    <dbReference type="OrthoDB" id="418131at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090158">
        <property type="term" value="P:endoplasmic reticulum membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029704">
        <property type="entry name" value="STEEP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057965">
        <property type="entry name" value="STEEP1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46355:SF1">
        <property type="entry name" value="STING ER EXIT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46355">
        <property type="entry name" value="UPF0428 PROTEIN CXORF56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25809">
        <property type="entry name" value="STEEP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STEEP1" evidence="2">
        <location>
            <begin position="58"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8168436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="195" mass="22442" checksum="BD35A44051930E65" modified="2022-10-12" version="1">MQDEEKILEQLKELDKAQDKNPVTLASLKTLKSAIYNRDVDLQTVVKLRTLNETTLKSENIKIYFCSLCGKKAIGANIGLDTLPTRRSDNSIAINLKQIFIRLFLKQEGIKYIKRSNSVEKQYRWCCEECGVHVAYQCVSYEEGAELIQGNSDIQLSNKPYLYVLNDAIVLNQQFSKLHSEIAKLKDQMEYEQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V2G3</accession>
    <name>A0A8S1V2G3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0540104</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8171085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V2G3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8171085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="194" mass="24442" checksum="ECAC558882D9E844" modified="2022-10-12" version="1">MYEILKIVDNSISLVSLTFQSQSFYIYNYVNWNYNIYIFMIQNEQNWYYLQQQYPQQDDPYEFYKLHIQNDLANFYYKIQEKITSILQGYIYFIQCYIQYWLFFYYWFFFEQRFLYNQHIIDKFNNTQQIFQNSYKIQIKSDFISKLNQLFGLHLELWILLQFILYKNANNFNTFSQSRHKLFLFLHNILKIQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V3K1</accession>
    <name>A0A8S1V3K1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0550067</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V3K1"/>
    <dbReference type="OrthoDB" id="1918685at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00024">
        <property type="entry name" value="CD_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051219">
        <property type="entry name" value="Heterochromatin_chromo-domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22812">
        <property type="entry name" value="CHROMOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="3">
        <location>
            <begin position="5"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8171521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="192" mass="22961" checksum="3BEF11943864ACB4" modified="2022-10-12" version="1">MIGLFVVEAIVGMRKNLSKIEYQVKWLGYSKEENTWELQENLVQNCSDLINQYHQNEFFQKFKGLITNYSEIKGNLRTDKPFQIVREFETQFLIAYYPRKNQFVGSSLINKSEAPIDLLQEFYCKQEEDIGQQEYKNQQIGCILDYYEKQSYVVLLESKERMFVDNQIIIDRQPEIMIDYLENMNWITGVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V4I2</accession>
    <name>A0A8S1V4I2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peroxisomal ATPase PEX6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Peroxin-6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0570042</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V4I2"/>
    <dbReference type="OrthoDB" id="2187at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000109">
        <property type="entry name" value="Peroxisomal biogenesis factor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF9">
        <property type="entry name" value="PEROXISOMAL ATPASE PEX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="475"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8172338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="702" mass="81826" checksum="8DDF57B5FCE6D061" modified="2022-10-12" version="1">MFVLTPRQINYTNNINEQYIIQASRNIMDHFNILAYGYIYLDNHLTIMINCNDEIKDNQIYISQHLYLQLKDPQNVHFVKANHSPYQYDDKNFRYNFFPNIPICKEVTLSIINNENSRLITQKFLKALFHVPIMIKEKLIIKGTLNDEQLLQRLQLQNCEISTKCRSKITNCCQLNNLLRDQYVYIHIDKIQAEKQNDWYILDFKVTNIKFQDEYSYSHVIKSTLDLKFQDIVYQLGDYDNFKKQINDIIRLHKSISIGVESRKNLGKRILLKQIANQNGILYFEKDFGLISSLRFTEKLILKIQDSPCILLLRNFSKIEQVLLLNKQHVSKDDISNEMIKIIKRIKKQKNVILILSNEDFSTYPILKSFVDFYIKFYPQTNVSKQLIEFYQLEQNEDEISQLLRETTWRVLEKEIIQNKQNFVKELESKLKTQTISSKTKMDDVGGMEGAIKEVAKTIILPQMYPELFDELVKPRRGILFFGPPGTGKTLLAKCIACEMKMNFISVKGPEMLNQYIGQSESNIRDLFKKAKDNAPSLVFFDELDALAPARGNQSDSNQVMDRIVAQLLTEIDNLSDGIFIIGATNRPDLLDPALLRPGRFDKLMYLGIKTDKESRVKILRALTKSDKFDEIIDEIPNNMTGADFYGLVSQATIYATKRTIKSGLNEMELAIEDLREALKCIRPSVSEQDLQKYEELKKKYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V4V0</accession>
    <name>A0A8S1V4V0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein arginine N-methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0570170</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V4V0"/>
    <dbReference type="OrthoDB" id="284431at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016274">
        <property type="term" value="F:protein-arginine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.160.11:FF:000040">
        <property type="entry name" value="Protein arginine N-methyltransferase-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055135">
        <property type="entry name" value="PRMT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006:SF53">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06325">
        <property type="entry name" value="PrmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22528">
        <property type="entry name" value="PRMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51678">
        <property type="entry name" value="SAM_MT_PRMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein arginine N-methyltransferase" evidence="5">
        <location>
            <begin position="132"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8172588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="302" mass="35386" checksum="198CC1F3CD89977E" modified="2022-10-12" version="1">MDNLNDEEIFNDRDRIEPFVKAITRNKHLFKDKVVLDLNAGMGLLSVLASQSGAKQVYAMKANCYATEIMKQNNIQNVTLKKENIKEVELNCKVDIIISAWMGNMLFYGGSIQEVIYARDKYLNKDGFIMPDKGQLYLQSIEDTQYRDQKMNFWNSVYGVNMKWMKQWVAKEPLIESIKDDQLNSDEQLIYEVNLQTCKLEDLSFSNQYQVKIKRQDYVTGVIVWMKYSFTYIHLPIHVIMGPTKSPFWKPVILYFREEIPVNKGDKLQGSLAVKFESEELLHIKLSVHLQQQNYISYFKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V539</accession>
    <name>A0A8S1V539_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transcription initiation factor TFIID subunit 1 histone acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0570094</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V539"/>
    <dbReference type="OrthoDB" id="21449at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040240">
        <property type="entry name" value="TAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022591">
        <property type="entry name" value="TAF1_HAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13900">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13900:SF0">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12157">
        <property type="entry name" value="DUF3591"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 1 histone acetyltransferase" evidence="5">
        <location>
            <begin position="604"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 1 histone acetyltransferase" evidence="5">
        <location>
            <begin position="793"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="1167"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="59"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8172440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1275" mass="151300" checksum="CABDC36331ED4913" modified="2022-10-12" version="1">MINEALLDILFAQFEDVNEMNQRDKSSLDINFAMRYFKDILDCDALFAGFKPQGQNEETSGDDDEEEVESEVEQKRITQRQNLKINEPNLQNIKLNPDMDQNQYLSSNPQLRTFLRQIDLKNNKQLEQTNNTRQSQNLKYPVYAFSDFYYAASEDVRMIYDKVINDILYKYKKTKSFDGDYYDSLMQFFSVLTDQQIKEHLQQNKDSEIILNSFKKQKCLKSLEKRLSLYTKLQNESKDNYTILCQEEIVDNPYGMIQSYQNPLLTPKFYQELNELINKKHKKIKLEEDINYKKEIQQKMKNENQINFQEVEENEEYQQQIQMDQDPKKNQMEMEAQIRQKDSENLRRFFTGKFKLFEYEQQQQQDNFNIEIKNHKNQRILNKESFLYQCPQENINALKCQAQTDEPGMGLLQRIQQQLEDEKKLNEQQNQQNQQSDEGFKQVEIDKKEQEFQKALDNYSYYNKEIVEFCMGYKENFLSKSFQTYNKVEIDLQNRQFFSEKLEVLLHQKKFPEKIGTQQQLANRLAAPSIVSRQKDNRSAVSHVNLLSVEDQQESQDLSQFEEGIQQQQDFNQQFGNEDVANKELLNLIESKMKAFQTTPFYNHSQVAESFLLNDFSQVLTKHAKYHRNNIIDNLYEEKRELLGLEREQVKYFDPWPTELVPLVNLKEQKKDNRDILLEESTEKQNSHEYYKQVENLSLSKGNFILMEYIEQRPLILNNTGMCSKVTRYVYSSRALQQFNQKRIKDEGDNYNNTQENSLQANYRSTYGQMGQISNEEMTLWEFKNMLVEQLGNIGQIRFLDKNGAIPLQGQLTDERLIGIAVIENDLYRAPIFKQKLPITDFLLVRTRVDNCHYKYTLRPIDCIYLVGQIEPKVEIFTPQSRPLGTFLKNCLKTYIKKQFKMGLEVKQDELELIFQKQNQKQSQKQSHTYIRSALKECKGIQDTSNQKTWRYQKGSNDDIQVLKYDITANQVCLYESMLHSSYLLQDFGLRELKNSDKMKKTLQEYMSRNPQDFNSWVIARRIEDELNMTAWSLSQSFLNSANQTGRMLLYGIGDPTSGHGGLNYIKKPQKARGEKGQNAIQEKKPQVMGTDNDLRKLHVEDIQNISSRLGLTTEANLKRWKMINKLTAFLQQSLNPDDKDSRDAVMFRKNLELLDDKQKKQLNVIEKYAKNQRQTTKKQKEQYQKELDKQLAKLIQNLTIKVQGKYFEDFEEVKKKRTKRDFDKDDKNKDLEYNDEDIKPYLIMNQENYKLRIEAFQKKEKKIWLEKKKKKGLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V6U8</accession>
    <name>A0A8S1V6U8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0570178</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V6U8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8172604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="139" mass="16281" checksum="07AE7CE20F74D6B1" modified="2022-10-12" version="1">MNQFESTEARILYLIYTLYQNNTISLDQKGILKDYLITKENGRFMNAITSFEKSKDIDQLQQFLIQFVEMENSISDFVSVNSFKTIKSQKPCDLFKETTQSSNTNSNPYIQFSPILNQKKDGDTQFISRKDRKYSTQIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VC60</accession>
    <name>A0A8S1VC60_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0590217</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000059">
        <property type="protein sequence ID" value="CAD8173619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VC60"/>
    <dbReference type="OrthoDB" id="293090at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="21"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="163"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="285"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="425"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8173619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="633" mass="75805" checksum="7E5664786A5E8403" modified="2022-10-12" version="1">MYSSIRESPQRHDTRSFVQEIEFLKAKCLELETENRLLFEEQQQLQRRAHDIVTNDSNVESYIKENNMLQTENEKLIKLSRQRKTDADLWKSKYENQLQQILQMKSNYEFEIKQLNAELQKLGAVLSQAEAERQRQLVGISGQIESQSNQDFENLKKATNLQIEISESQIRKLRDHIDEQNNEISDLQQKILRQKTEDDIQIERLLKENELLRVKIHQQESEKQRELEHMNDNLNNFSQQQIQLLKHEFARQSDVQQCEIDKLKGLLEIKNAEIETLLGQNAKNKQMFEDQINDLRTEIQIQKQKLLDQERQSRMILETSLKDQQNQHQRDQETLKSYYQTQINNLEKEINDLKGIIEHKNQQIQIQIEEKNIQRQQLEQLIIDLRREIENQKLITFEQEKQKNNEINELDDQFQKSTSLLNENIDQQKLQILFLEGEIERLKEMLSQKTKDQESLIAQFNLHKRQLEDDIRRLKDEIHSLKQEILSITSSKNQEIQDLQSKNDRVTIQYREKLRQSDISSEQQLLEIKRLRETLSVKEQENENLANQRNLLDKDLKRARDEIEQFKQRQLALEREKFTQLEDLKNKLEASNSYQISNLKAAYNTQISVLTEENVHLKQQIDQRRMYDSSVRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VKG8</accession>
    <name>A0A8S1VKG8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">Fructose-bisphosphate aldolase</fullName>
            <ecNumber evidence="5 10">4.1.2.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0690201</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:14729"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.1.2.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the class I fructose-bisphosphate aldolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.13" evidence="5 10"/>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VKG8"/>
    <dbReference type="OrthoDB" id="36455at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051289">
        <property type="term" value="P:protein homotetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00948">
        <property type="entry name" value="FBP_aldolase_I_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000187">
        <property type="entry name" value="Fructose-bisphosphate aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029768">
        <property type="entry name" value="Aldolase_I_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000741">
        <property type="entry name" value="FBA_I"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033379">
        <property type="entry name" value="FrucBisAld_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11627">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00274">
        <property type="entry name" value="Glycolytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00158">
        <property type="entry name" value="ALDOLASE_CLASS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="8 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0704">Schiff base</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8178448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="359" mass="38940" checksum="8C7DCDFA10218DB6" modified="2022-10-12" version="1">MQQKADYRKELAETAQKICTPGKGILAADESQGTIGKKFVTINVENNEENRRAYRELLFTSPGIENYISGVILFSETVKHATKDGKNFVELLKEKGIVAGIKVDKGLGVIPGTQDESATLGLDSLANMAAEHYKLGCRFAKWRAVLKIGNGLPSQQAIQENAWGLARYAAICQENGLVPIVEPEILADGDHSIDVCQKVTEKVLAAVFKALNENNVFLEGCLLKPNMVTPGSTNTDRSKVTPQEIGYRTALALSRTVPPALVGVTFLSGGQSEEEASLNLNAMNQLTTVRKPWALTFSYGRALQNTAVKTWAGKQENWEAAQQALLTRAKANSEAQLGKYQGGQGGASNESLFVADYKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VKW3</accession>
    <name>A0A8S1VKW3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0700026</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000070">
        <property type="protein sequence ID" value="CAD8178628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VKW3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="347"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8178628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="393" mass="46830" checksum="526037C3393D92FD" modified="2022-10-12" version="1">METVSQHITQLNQKNQELSALENRINQLKQQLNLQQANNNILKTKVDKIVYSKKQKFYDQMKLKQMLQSSDVNNDGVQYMVQRNKALLNEELRQNKQNMLEEKYLQTQQMKQKLDLEMLINQKKRDQQLFQKQQQYAKISEWEFNLKLNLKQREIDKIDKIRLEEKEFKEQLAQRMLEQQQHCDQLSNEEQQLMSKLKSSQQFENSLKYDLKTAMSLSIKDYTNNASVSIPTYFKSYSSSKLLKQPLNSPYAQLPTMLKNKMSESINKSKNNNESLMKEYQFSLPSLCKPSFIEQQQQVERLFQSKSVKNSKSDLHQILNYCDPKGIVEKKSKFQINQSEVIQNQVSTQSIKQNRQKSQEQEQNKEQLIQEIQNKQEVEKLNQNQEEEEEEYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VM61</accession>
    <name>A0A8S1VM61_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0650200</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000065">
        <property type="protein sequence ID" value="CAD8176672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VM61"/>
    <dbReference type="OrthoDB" id="301536at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8176672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="78" mass="9239" checksum="8BEE79C8662D0DFD" modified="2022-10-12" version="1">MGICHSKSKLKRSGAFHCIPIDFDKLKKYNQERQNNDLNIVKQQSFCQLICQDANKFSNPQTQTYNYVQTQQIEFKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VM63</accession>
    <name>A0A8S1VM63_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0680213</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000068">
        <property type="protein sequence ID" value="CAD8177997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VM63"/>
    <dbReference type="OrthoDB" id="290333at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8177997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="303" mass="35835" checksum="F05B69032273727B" modified="2022-10-12" version="1">MDLNFYNNSFFYQCLRGPQSNRRNSTPRKRHKLLQPTLQLDLSLLHHNSNLADSAIVTVTRNRQSQDVPNVQANDVSKKESTHKKSMIMNKSSNIHRLYTQINDINRQPILLDHTKDQPDVILQYLPQGQEFINKNNKSILQAWIPTLSQEERKQIEKNKKNFPIPRNITYNEIMDGLNKLYGKGKNRRLWPGEEALLMETIRLYIDKVLQNPRLLMQSVAFTLLQIITISGLTEIANYKLCMLQLECHWKMMITRFRYSGNKELNKKHWQHWLELAQLRGHFLNLHTDNVLTQYLLKHQKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VWP9</accession>
    <name>A0A8S1VWP9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ABC transporter family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T0700050</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000070">
        <property type="protein sequence ID" value="CAD8178676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="294028at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18580">
        <property type="entry name" value="ABC_6TM_ABCC_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000610">
        <property type="entry name" value="Multidrug resistance-associated ABC transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000140">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000151">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002273">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044726">
        <property type="entry name" value="ABCC_6TM_D2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF456">
        <property type="entry name" value="MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN LETHAL(2)03659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="302"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="379"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="405"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="494"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="915"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="957"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1043"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1141"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="269"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="608"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="961"/>
            <end position="1169"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="1231"/>
            <end position="1464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="19"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8178676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1520" mass="175175" checksum="C3BFD5D8BA8EB119" modified="2022-10-12" version="1">MLNSYTNKQRKRFQENRSRYLQQAQPDESVSSNTELEPQQQDIRVRLERPSKKKVSVKIRNLDIDSDIIKEENSEDYASEKQSKDQINHQESNAVLNLIPEEEIDSPFKSRINQNKEILWKSSNQESQNVEVQLFNTPKQQQGADSPKIENAIKSYKNLNTMNSLYSPVKASQKLIINDDTIYQHYNIFQRLFLYHIFCYMKQMKKFIESSTQKISVAHLPKQAYRDSIQYNLKRVYEQIKNNDLYNFNNRALFKLFFWQDNKYVTMMIVILAMFETYSRFVMAILTETLISAITENDMNSAYLQATALAILSLLALMSKHQQQYLISNFATKIRMVFINLIYDRIIELNSTQISMLNVGKIMNLVSSDLNVIEYQLAFIYQVAVIPGSLIFTSIILWLRFDGPLGLVAIIFCAILYPLQILIQNINKKLLLQTRKLQDQRIQQTNTVIEGIKYIKMYVWETIFENKINIIRKKEFFQYLNIHVLNLLDRSFNFSVHIWGSFCFILILYACDTHLTISTIMGTIQLMSMIKYYCIFQVSYAFQALMNFSVIFSRVSEILKQQSNNITTIDQFKTTFTTYDIQHQPMQQLLMDRTRGLNRKGSLTLLSTSLVTINQYSGRWSKDGTPVISSINLDIKSGEIMGIVGKVGAGKSTLLAAILQELPYYEGSIIQSKKLKLAYVEQEPFIYTGTIKENILFGKDYDQVLYLKVLEVSCLDQDILSFRQGDKTEIGEKGANLSGGQRARLSLARALYSQADLYLFDDPLSAVDSKVAGKIFDNAIKDFIFKFQPNYRPTLIKTHQAFIQQTPAVILATHQISYALECDYVVIMDGGMITHQGQKNKMKKHILEITSAHTSNTNVDNPIRSLKIRRPSKLMGKVVNQMQKSNKDETQTLYITEDQNQSDATFDTYKRYFSYWKPFILIFIILCQNIASEIINNYYYKEMASFDEDRQRDNDQIFYNAAILVLAAYFNNIIKYFLNIFGVLTSNNTIHNQMLKRLILSPITYFDTNSSGRLINRFSTDLSLADTQIQQTITDIFEQGSQFLVSLVTIAILQPYFTFAAIFTIISTILIFRITRSVVSQLKICDLIQRSPLFDQFKITINGVTQIRINENQSWIKEKFIKLSNQSMQANLIFLYSQRCFGFYIDLFGQFANISGIFLIIGMVSDPTIFSQALLLLSTFNTQAGTLRQFMAFDSMMNSVNRMFEICDLEIEKEEPTGELEIKNWPKLGSIVYSNVKMQYRENTPLVLKGMNFYIKDKEKVGIVGRTGAGKSSVISSLFRLNSIENSGVISIDDQDIRKINLYKLRKEISIIPQVPFLFKGTLRENLDPFQNFDDKTLLNVLSDTGLEGFIQQLPNGLEHQIEPEFFSIGQKQLICLSRVLLNKKKILILDEATANVDMITDFLIQQIIKDKFNDCTIFTIAHRLNTIADYDKVLVLEDGKVLEEGHPYELLVQNPKVSTYINSDSAFAKMVLQTGHKNSSQIYATARRSYLEKNEIQSKSNCNSNILDVSLSSINEFNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VX66</accession>
    <name>A0A8S1VX66_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0770165</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000077">
        <property type="protein sequence ID" value="CAD8181940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VX66"/>
    <dbReference type="OrthoDB" id="418560at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051216">
        <property type="entry name" value="Teneurin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11219">
        <property type="entry name" value="TENEURIN AND N-ACETYLGLUCOSAMINE-1-PHOSPHODIESTER ALPHA-N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11219:SF69">
        <property type="entry name" value="TENEURIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23106">
        <property type="entry name" value="EGF_Teneurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035923369" description="EGF-like domain-containing protein" evidence="5">
        <location>
            <begin position="18"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="402"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="406"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8181940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="479" mass="55638" checksum="D431B27A6F6D2A6F" modified="2022-10-12" version="1">MIFLLIIKITIAWKSESVRENIQTCQIFQSSIQRECEQNDLIIFRLDSYFAICLISSEIGIQSVLYENSQGNNYFICVLNEQILIYSLPHTCGNSFMLLQHKNKFTNYSLTRDDQFIYIFGGLNDSKLRNTLYKYNIQDLLNNQITFSVLPNNFIQKLSVSLELWDGNMEEFQFTSNFPQLQKVKNLGYKYIIYSFQDNSQCSSSNIPKCSQNSVIVYVDECNCIKLFYGRDSNNINQNESWIYYLDELIWQETTVDSMIKFINTPIGQYLPEIKIVGFFADNTYFFYYDYQWYNNYEQLNFGSTFKQMIVYDNKTFLLNSTSILYSQNDLQNAKSQQYGIYVCQDDYHGYNCQSPDFQCPGSICFNDLLFERICVHCQGHGTCQYGKCKCDDGYNGDDCSQYEKCLNDCSNQGSCIQYYPTPQCRCKQDDKRGGEDCSTIFCLNDCSNNGICKNGICECIVGVKGDDCSILNLKFIDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VZ73</accession>
    <name>A0A8S1VZ73_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0790039</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000079">
        <property type="protein sequence ID" value="CAD8182630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VZ73"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8182630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="66" mass="8264" checksum="3588F362FDE37A69" modified="2022-10-12" version="1">MGLRLSLLKTINLYLYMVVKQQINFMYLNQKRESFKKIWKRLLNQLNIIRSQMNITFQQFIKRRRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VZX8</accession>
    <name>A0A8S1VZX8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0760127</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000076">
        <property type="protein sequence ID" value="CAD8181412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VZX8"/>
    <dbReference type="OrthoDB" id="291504at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8181412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="263" mass="31178" checksum="C37C1517E4AEEDEA" modified="2022-10-12" version="1">MSNCIVKEEEQHVQTPMSISKTTISKQNRRKVLQENQSFQSIYLKVVSKFLQTFHNLQMAQFNSQQKSFPSYPFILQYSFNEFLVHFGGHKMDFQYTPVLNNFQQQLQIQQCVPKLITQQCPSIKLSIQKRKGVSSQEQKDTKNIPKNYCKAIITFASKNQALCHQILGDQLKVVKFLERIIVYKKKLMNIRIFSALLSQSEDPDEEEFNQTFRILSRIFVKKYAINYIFNSKIVQHNWHLRYRLQIYKGIKNPKNFSHIKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W320</accession>
    <name>A0A8S1W320_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0790060</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000079">
        <property type="protein sequence ID" value="CAD8182662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W320"/>
    <dbReference type="OrthoDB" id="2942533at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="146"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="180"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="214"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="248"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="282"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="316"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="350"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8182662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="421" mass="49539" checksum="600A92533EB6C306" modified="2022-10-12" version="1">MKFNKVSQNNNNPQNKQIVKQNNIKPAQQIENSKNKELFAQSTETLITKQNQKVPLLNHCYHQKKDKNDISYNQKGQTQIKQQFQKFGNKHNPHSELKVYDKKAIQINLNNNQSYMNKGLELFNQNLFEESLVFFNQAIIQNSNDYLAYSKMGNALIELNRHQEAIEFLDKAIKLNPKDDINYINKGGALYELNLYYEAIECYNEAIKINPNDESVYSNTGFILIQLKKYKKAIDYYDKAIELYSKNASNFANKGFTLIELNRFQEAIECYDKAIKLNPNDDCFYERKGHALFRLKQYSNAIQQYDLAIKVNPRDYHLYLNKGLALYELNQKEEAIQYYNKAIELNPSDYKLYLRKGNLLNQLQRYDETIICFQEALKINPDFFFSYDDLGDYLLQVGRKAQARYCYLKAQCDPLKILKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W7J2</accession>
    <name>A0A8S1W7J2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0840236</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8185230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W7J2"/>
    <dbReference type="OrthoDB" id="299495at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8185230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="105" mass="12028" checksum="E08FFA68730CBF87" modified="2022-10-12" version="1">MISQSPGFKLTPMTPLSIHHKMEIIEDEPDDLDLDQSKISFNDNLIVCTYKPCQSVTMITKTISKLKGQPNTEWVNPSLRNEQRKRSTSILKYSQSPTRISSIYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W8A9</accession>
    <name>A0A8S1W8A9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0860221</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000086">
        <property type="protein sequence ID" value="CAD8186224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W8A9"/>
    <dbReference type="OrthoDB" id="10340424at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="469"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="510"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="580"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="639"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="687"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="768"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="18"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="792"/>
            <end position="821"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8186224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="821" mass="96230" checksum="0C128EEC3BAFA4FF" modified="2022-10-12" version="1">MSASRYISKKLQPFITSKLELTQQLEKLEKDQKEEENLSDQYFDQLGKVEKEYETLVHILEEYGKRTTVKQNGDTDQAQNLDSRIEKAVIETLSLYLLGNSKKEQLEALQEREIAKRLSSINLTLGKNQIDQQKIIDDDNQLNENEYGYFVTLKSDDTSIDVKIPATIKTFKELKQIIKSCFMLEENEIFCTDLMGNLFQDNMVLLDEIYPPLYDLMKNYQPIIGVQVVKQVKLKEIVQSGDESLLSDDGARYFIKRLRTTKPVQKKINWNLYLSYLNNFKYFIETVLFLMVLGLFLVVQIDSIKFTTSSSMITSFQKQMQISKSFLSPINNISQIINQTLTYTDDTHKYPTYPLVSGLLIQTLVKQEKLQDCSILNQHQKQMFIDNNQSCLDFSILLTDNLTDEYEFNNFNPSIYNQQFGGYVWELNLTSQQEFSENIQLLFEKNWIQYNIKSSKFILNYFNTPTKRLIQVVITTIYLFNDILLNYNSINLESFDFNKSTETDDFYDEIIFYLSILLLSLSFLDFFAIYKNNSDNPFIFLYNSYSELINKQKKLQSKKKELSSVEQQELQQKELIIGDYFIFQISVVYVVIKIPLIFDFVYILSNITILIRGTIQEIYTNALDEIQINSNVFQDASKLIVPAFILKIFTGVQILLLMGSIIRFLGNWSPYLKCYGLVMIRFNKQSSFLLLVLVFIISISAMSWSITIQGKLLYQDNFFYDFLGLLRCSLKYGMHNDLGDLGLENNYQKQISYAFETRYVIKIFKIKLQYLIILVISFIMVPIFISLMTQQVHNTKLEAEQKMIEMQKEKEEEEKQKQKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WCR0</accession>
    <name>A0A8S1WCR0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0870145</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000087">
        <property type="protein sequence ID" value="CAD8186577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WCR0"/>
    <dbReference type="OrthoDB" id="304343at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="10"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8186577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="203" mass="24129" checksum="29991A52BC2CB9E9" modified="2022-10-12" version="1">MLQSNFTSYLEGLEKQITNNELDYDEATEMISKLNPQNLMEKKELMQIKKKVQQLKKDVAKEKIKENLNSHIFLLRELLEKSRNVNNSIERYKLLSEAEEQKKITEALIYTLNQYKDHISQKFVGDCKKVIFEYEEELRQSFVSAKREEVIFITNQKSFSQSMFVSEIEQNEGVNQLTNKRSVRVKIQQYFNKMFGCLITYNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WM06</accession>
    <name>A0A8S1WM06_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0940176</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WM06"/>
    <dbReference type="OrthoDB" id="1926212at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050498">
        <property type="entry name" value="Ycf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE SPINDLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="262"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="331"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="873"/>
            <end position="904"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8189737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1068" mass="125671" checksum="AF681C2E2332A6AA" modified="2022-10-12" version="1">MINSQQKTSSTRFQNRQRSTDTYLNSQILKLQSQHQFQQLLQKAYSMHFQTQQQEFPKSKISQFVKSVIQKQPKTFLQESKNNISNVIQNVNFKNQYTIIKNHSNKQHSISYYNNDNSYQKENEDIMNYVNHLSNISFKDSINKQGNLNQVKYIKQCTENSKKIVEGKKMIRQGKIKEALNYFEKVIQTCDDPEIRFQIAIQYFDQQEFSQVILNLNHLIQEHPLFNKKAYIILSMAFKKLNLMNESLNILTICIQHFAKYFDAYIIRGKLYIKIQKFDKALGDFMIAIELQPNKAVGYIGYCDCFRMMNNVEMSIQAYTQGIKLEDQFKRSALIKRTLLYLEQKDYESAMKDISQVIEINDQDSDALYLKGFICTKKSEIQEATLAYEQAIKHNNSKKAVSKSLYELAKRLIQQKDFYEAQYQLRRAEHLDIDKRFIENIKQFTEGVILLMKTKFEEGVNLLTELISNHQIEDFLKRLILQYRSYGYFCLSQYQQSLQDLDSLDQLEKPSIYNKLILEGIMSTISNQFELSLGYFVKASKLMPNKIEPYFYRAVTLVQFYCQLIPKDDQERKNKFLNDAIRLLNLALKINENSNLFFCRGIILFSLNRLDDSLSDINKAIDKSQENVAKQFYVRGLVQVCRQQYKQGLNDFSIAINLDESYSEVYLCRAKVFLILKDTNHAYYDLQQFQESCLKVQDQILIGNLFYQMGAFEEAIQSYSNIINSEKCLQALYQRAKTFITIKELNNAIQDLQKLVEYSNDISPFVDLNVLQQLKLTSIINCGQIQLSNSIDCLNQFLKNGNEGKIFKTQDIIFYRSLFEFYLKQFDTALISFLESYQIYQTSDNIIIKQQTEQKHINRVYNQLEFQFNLSILQLLSGQNSLAIENLKQLKNNLQEKEQKNLLQLFLELLIDDQNEETELRTELSQLTNLIILSQDNRLCNIYPMIKVPLKKYKQNLQTRLSFCLPQIEIPEMLPQFDLKLLETISLLLVENKPEAPWIQRLDNNGVIFTQDMYNANDLDLKSTTRQSNGESEFQQFQNQLNNKLEKEDFVMFGQDVFKQQTNIYKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WNY4</accession>
    <name>A0A8S1WNY4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0970179</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000097">
        <property type="protein sequence ID" value="CAD8190912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WNY4"/>
    <dbReference type="OrthoDB" id="6252103at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001434">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="180"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="225"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8190912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="444" mass="52458" checksum="FF352AD61C529E33" modified="2022-10-12" version="1">MIAKQVLNRQLEFQIQEIEQYLDSSFDNINNFIDQIFIKDSLISELNNSKLELQLQLPKVPQFNLIFQSELQSNIVQEIKPLINYKTIHQVQIKQSSDQKHQQSLIQSQQSQFKQSMDPKSQFNSKPFKYHLIKHNSIKQREVCYTIAFNKDCSILAIGCKKQIKIYEFKQGLLKLIQVLNEHKDNVFTLNFMKKSNQFISGDCVGSILIWSNNINNQFNCQQKIQGHNDAIRCLILNTNEDILISSSSDETIKFWIKQNEWICQQTITNHTNWVDQLSLNDQQNQVISCGRDKQILVIEYSKQNLKWIVIQNILVEYNGRRLCFINNNQFTFQPNQGNLMHVYEMNSVTKQFTKTKDITVNQNYDDCGLFPQQFIKQKQLLVDKHHKCLNLIRKTQNDEFKVEQSIQFDDYCLYGQMSDDGEYLITWDKSSKEIQIRKYTEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WRK0</accession>
    <name>A0A8S1WRK0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0980024</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000098">
        <property type="protein sequence ID" value="CAD8191005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="190265at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="13"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8191005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="300" mass="33372" checksum="180CFA854E54FD2A" modified="2022-10-12" version="1">MNQINPDQILTSEEIKAAKDAFEAYDKMGYGTLEVEELQKILXNAKLGRQVYGETQFADITDEEFREQYLTLKYSPQDYANEPRESFSHITADPKEIDWRQSGAVTPVKNQLKCGSCWTFSTTGVLEGFFKVTTGELPSLSEQQLIDCSTTVDLNFGCNGGMPQRALNFVKRNGLTTEEGYPYQGVQGAECLVKGGAYKVKGSKVLEISEWALYQALQVQPVSIGVDAGTWKNYKPADREVFNYDECGDNLNHAVLAVGFTPTATIVKNSWANTWGSSGYIYLERGKNTCGLWNTMVVPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WVI6</accession>
    <name>A0A8S1WVI6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0940203</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WVI6"/>
    <dbReference type="OrthoDB" id="167576at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8189786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="244" mass="28831" checksum="0089CB3136E0E8E8" modified="2022-10-12" version="1">MSEILKNNLKMFNVDDSQDSRVGLGKLISLLEILNKEFLEIVTFYHKLVLQHSDGQKQLRIFLQVKEIIMKNYMLLDYVQMVNGNWMILFLIFQEYRFRILGFIIRKTWAKMNIDYTDIEGGDPREVIKNIWKSKILKILLINFCCLFQKGIFIILFNNNQQLDYFVLWKHLFLKDQVIFIIRIIHKKPLSQRKLQIINKVLNSQSQKLEIIIQMNLAPGKDKIVLCSLDPAGVVFAPKNQLVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WWE5</accession>
    <name>A0A8S1WWE5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1020088</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000102">
        <property type="protein sequence ID" value="CAD8192655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WWE5"/>
    <dbReference type="OrthoDB" id="308451at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8192655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="605" mass="72932" checksum="580172EA93CCBA28" modified="2022-10-12" version="1">MNQFESNQIGSSSDSPSNKQKEQKIIMILSFLTFKNIVFPKILNHLFFSEQKGIIKIRKTDQLLKLLTKTYNIDLIEKFSIDVPEYTEIELIVKILQLCLENIASLTDEIINSKAFFSFYYKLYDYQKKKQNNNIILEYVQQIIQQTPNIQIITEDFKYFIQFKAQMISIIQSLKLRIPLTSLLLCTRFYMNRNTQQFFIEFLITNEVQLSNFNYLMNIDQLINQNTLSLSDMNYYIDKFFPRYEDELIETNFNIFSKNLDLILEQKKYMIIDLQQLIESKKFKELYNELQKYRHQVDQADIFYQGLSQIIQYCQTSEKSIMDFSPVNIPSFIKKNFFRTHEKRELIQQQQIESLLIFNKILTEMIRFRFPFPIIISYLDLNNSNILINISKELIIQCQQDKVFNLFLALVHYNFQTLAKSQTYSKAFYHSQLEKQEMQQEEIIFQMLEIILEFKYLIKNDLEKILNEEDTKCKINNFIKYLKNKNIDQNKFEQKIIYILSELIQFDEAKVVDMNNYIQLKNQLQKTISEIEYKTTKQPYQERRLGKPPGFYQYQQYIQKRIRSNTQQQKQIKQPKDQYQIQKHKKINKLISEQKLMEELESILV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X3K3</accession>
    <name>A0A8S1X3K3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1090048</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000109">
        <property type="protein sequence ID" value="CAD8195310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X3K3"/>
    <dbReference type="OrthoDB" id="303381at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="130"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="277"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8195310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="500" mass="60261" checksum="ABB29F0A755FED30" modified="2022-10-12" version="1">MIDQTSPQSITTQKLCLRLGYDPNDLIYKSIEQFPSVSPEVQQMKYNHYKNRIMRIINEIQATKQRHKLGDQSLKQITFNQQDNNFSNFSEESVMIEEKKEKKSLSRQKILQNLIYQQLEGEKKNVEYLQKQNQKQQHKQQRKSETSFNKKKERDSYKNKSNEEIDEFEKRNQYFITCIENESRIQEQLQGRFLKKNFKHDNYTERAQEKRKQQESLFQQTITERLNKLQQKETQAQTKVELYKKDMRNYFALKNSQIINKQEKRSQLIQFEQQQYLKEQCEKMKKFQENKSQLILEEKKKYKEKKEKYIQMLRKCKESNFEQQNKHQEGFSNTYQQLIQKQEQVIQNKERLLEQKSLVLKDKLQLNLEDVEFNRAHAQRIKLNTQVKLNGNKDAKSQQSSRTSDTWRMKTQLIQKESEISLWEKAKVAASLFKDPIQYEQYVEQQQMILLKKKKSLDQKFAQAASIIQEILPKNEAEKLLMPSGLKTFIEQQSRIKTQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X472</accession>
    <name>A0A8S1X472_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sulfide:quinone oxidoreductase, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T1090097</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SQRD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000109">
        <property type="protein sequence ID" value="CAD8195395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X472"/>
    <dbReference type="OrthoDB" id="5376590at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070224">
        <property type="term" value="F:sulfide:quinone oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070221">
        <property type="term" value="P:sulfide oxidation, using sulfide:quinone oxidoreductase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000034">
        <property type="entry name" value="sulfide:quinone oxidoreductase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015904">
        <property type="entry name" value="Sulphide_quinone_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10632">
        <property type="entry name" value="SULFIDE:QUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10632:SF2">
        <property type="entry name" value="SULFIDE:QUINONE OXIDOREDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0874">Quinone</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="11">
        <location>
            <begin position="20"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8195395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="422" mass="47820" checksum="5EBF1D3285C9AE11" modified="2022-10-12" version="1">MLKLHYLRGYAFSARQVHSQIAIVGGGTAGLNVAAQLIADGHAIPQQIRIFEPMKMHTYQPGWTMVGGGLYDINKTLKPMEKVLPKNVIVSDSPVIKINPEENQIHTFDGDKYTYDQLVVATGIRTDFDQIKGARQYLEDPNSPVASIYHYKYASKMNRLVDEFEGGKLVFSEPQMPIKCGGAPQKILYLSHDRLRKRGIKSDFHFYKTSALIFGVPKYAEILTDVCQRKQINCHFKQKLVEVQDHVAIFEDQDTKELKSVTFDLLHLVPPQVPAKFIAASGLSDAAGFCNVHPNTLQHVKYKNIWSLGDCSSLPTSKTAAAVMAQTPILVKNLIRTWKKGLDPIPEYQGYTSCPIFTSQSTLLLAEFKYNSELDETFPIFQSNESRLMFYLKKDFFPFAYWYFMVKGLWGGRDGFRFFKGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X4N0</accession>
    <name>A0A8S1X4N0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1030193</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000103">
        <property type="protein sequence ID" value="CAD8193246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X4N0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8193246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="117" mass="14154" checksum="FCCDFD5ED4470C2C" modified="2022-10-12" version="1">MLIITINNGQKPYQIYEREQFENVKLIKNKLQKEKQILLPEVLEYHDQLKQQVMQPIQLWRVNNSNLNYNYLIQKIKGFVSQIELDQQQFIFYIFPKLQQLEKFHSSPNTSEAANWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X620</accession>
    <name>A0A8S1X620_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA polymerase epsilon catalytic subunit</fullName>
            <ecNumber evidence="6">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1110130</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="6">DNA polymerase II participates in chromosomal DNA replication.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="6"/>
    <dbReference type="EMBL" id="CAJJDO010000111">
        <property type="protein sequence ID" value="CAD8196109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X620"/>
    <dbReference type="OrthoDB" id="10060449at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008622">
        <property type="term" value="C:epsilon DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008310">
        <property type="term" value="F:single-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006287">
        <property type="term" value="P:base-excision repair, gap-filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045004">
        <property type="term" value="P:DNA replication proofreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029703">
        <property type="entry name" value="POL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054475">
        <property type="entry name" value="Znf-DPOE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10670">
        <property type="entry name" value="DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10670:SF0">
        <property type="entry name" value="DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22912">
        <property type="entry name" value="zf-DPOE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23250">
        <property type="entry name" value="zf_DPOE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA polymerase-epsilon zinc finger" evidence="7">
        <location>
            <begin position="83"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF22912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8196109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="153" mass="17997" checksum="B6579DAC3A6E434F" modified="2022-10-12" version="1">MKKIRNVCCKIVNISESSKEAEFEDPCFDLILQNVPCYKCYMVSEVNVFKDLWKCEGCKDDYDSQTMEKLICQFVEQQLLFYQIQDLYCVKCKQTQDYSFQKSCQCSAQFQIKLDQFEKCFIRTPTQLFDSLLQLAIEKNLHGLKTLISSINI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X8L7</accession>
    <name>A0A8S1X8L7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1150052</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000115">
        <property type="protein sequence ID" value="CAD8197330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X8L7"/>
    <dbReference type="OrthoDB" id="294423at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8197330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="158" mass="18311" checksum="B47C0679D88EEFAB" modified="2022-10-12" version="1">MFNYSGMLPKSQSLYFHPPLQVLSTRNVQTEPEISKQQHKPHKKYSVITDDKRNLLIKQVLEEGVSVKLAAQASEIKLSTAKAILKTYKTQGRIGKKKERQKRPKLNRKTVEKQLDQATLIKKQLPKLGQNFIYLPYSYQPPPLIYKIEYSNDVKSEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XCY0</accession>
    <name>A0A8S1XCY0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1200012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000120">
        <property type="protein sequence ID" value="CAD8198815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XCY0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8198815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="338" mass="40624" checksum="EEFABCA3CF341815" modified="2022-10-12" version="1">MNILILLLNNSSKIQFKKRILLVHNSQQQRKKYQNFRLIWQYQYNEIINYTKQQNEYNVQKTRTMSELYRQNYKDIVFKIINRLNLQIYFIAKVLYQEVIEFGKNTSTLPCYLMKQIYVKKIIQEQLLYLKSNSQKHLILNRQYQRSSSNSINLLSILKLFLEQENQDEIVISDKKSTVLVCLKQVYVIYIGKILIKLNKFQIRQNNRNRRTLNSQRKWNYIVSIGGKEQYFWTKKSEAISNWNINKILEICNSNNSQIKRNQFDKYNYISFLRDNSIISVSIEQNQQDQNNSINSQIMIIDYDKVELISKLTNDHNCNSICFLTSKEILCQHFQILT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XFK3</accession>
    <name>A0A8S1XFK3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1220188</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000122">
        <property type="protein sequence ID" value="CAD8199867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="436852at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15489">
        <property type="entry name" value="PHD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12460:SF0">
        <property type="entry name" value="CID DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12460">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="7">
        <location>
            <begin position="303"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1890"/>
            <end position="1925"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="865"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1292"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1820"/>
            <end position="1883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1890"/>
            <end position="1906"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8199867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2199" mass="262748" checksum="A8F84C8C5C29B9D3" modified="2022-10-12" version="1">MNEVDNKELDLSVVQHHLNKQHSGLRLKKQPHFKDKKAECCEICGIGFDRQIQQPEIGMMIITCYGCLSTCHYYCYGISTEVEKLSNSNNQGYFQCDKCKSNKSQDYPCVVCNQKQGLKKKLHNSNEFVHPLCAQFSKQIEIISFIEMKFNKLNNQNISQIEKLNTEKIKQNQYVCVYCKGSIGTIKCNKCNSFAHIYCIIHKITDSGGFRLDYSESNKDNQWQCFFNYKQILNLDNMIQTNKDKQLLKNNVQIIFDQFMKIQGISKQIKFDTLFQQYFQNDKIEEEKESILSWICFKHKNTKSYCYCKQEVDTDDMVQCETCTEWFHDQCVRKYQKDFQEQKNKDIYCCPFCIKWAKNKLNHILRYNPVSNLNQLFPPVLKLNFVSLIIIAIYCERTLNRMISKYNETEYKIVQFILSNLPFPNSLQTQLNQIEEKYKSSDIINFIFSQNIVYRDENQWIIKSQLDQNNNTVPENIQHNNNLKNKLLQVITLFKDFDECKYYQELLSKLNHLQNAFEIIIMKQKITKNTILMEYQSNAFQEFIYLQQQIPTYKKIKKEFISTLQQSQDKFYDFETKFNGIIDSSEEDNDDDELEDYQKLQKYFQLDIVPIQNYQEFFNLISQSRIKLNQFKINIEYVNKILKQIQNLSIKVSWISYIEQEVNQGLQIIGQLEDSKLNNQNNAIQILINKMEQTFCYYKDFPKHYAFLSKIKLYELYIQDLDYYQDKDDEDEDDDDSELQQITKEIQIKISIPHLQMLKDLQKETRAPQKEIQLLNRVEQQMNEYRQKLRNIILQKGLNEQLRKKYQAELQMNKLYDIPEIKELQQKLEIFQEVEKIRLSKTQTLQQLEMCLEKSKQYNFDDKIINQFEQEINKYNQKRKEIQKLLENEIYESEQLDQAKIYLQEIAQSKIVFEEKQQLQSLNKSCQFVQQLYEFYQKYKSPEQEMEIEDDKNPDLQFKINQICNPLQIIFKNDQESMIYDLEVILNKQQIILDKRINNIFNQYTNFLWQKQAKILLSQYESKQEISNQLVDSIISKKFKLQDSLAQNKLDQFIELFNKQLIILNQIIIPLQDYINKQPKEISLSEWIEGYNQITLNIKGGIWDQVKIYNVWVTIIIKFQEIENSELHTYESLKLLQEIIQMSHMPKYSAIIQIVNQKINNFNNLVDRFKEYGQRKQLFLEKKKSKQESLSSKQKFYFSILDAIDLEQKLKRINNMVHDFFSKEFCTDLEYIKQIQEDFNNCQDDDIQIYSQLYQRLTSSFIQDDFLLDQLKIKIYQSKLKDIIKGRKTVELNKAKKQYKSLMNLMEQTNNEFEEYIQQFNKLLTIGENVQNIINKVKNEQDYTFEELELCVQDMEKISNIQIENKEDLLIKYQECLQAQKEINEIQNSSIKSKEMLIQNLYEKIEQLPLQEEKQLVQTWLNQYEQLRGNYITIKQMLQKQKTFQSCQLLTELIKKLQKSNEDCVIIHPETENLLRDYQEFNKRLEQIESNPQSDNAQKEFQELQSNLRWGEQYERVRMVIWIKQINQLKSGQSSKQGFSSFRNLLNQGYEILDSALLNNQKLQVEKIKGLIIYLEQLMSKIIEIFTNGINGNQIIEGKIDISQLILELQHISNHQKQEKLKDLQPKKPFDDINQYLNKLQMKQKKKIIPSTAKDKPEHSVKKVKKSNYDNDYEKLRIEKRNELFNVMKQIPILKDRKDLNDKIKEIENQQFGEYVKNKSKYEEIMQLIIETYKELQKFPNYSKQLFSSQINLETTAKAMQKYQDGKLQKCEDKYKQVRQQDQNSLSQIKNNIKLQSQKIKLKSQTQLLTQKQQPQIMKLEKLNQQLNDSCLLIHKLQEQQQLQQQQQQQQQQQQQQQQQQQQQQQQQQIIQLQQSYKQQQSKQKQQDQVLSSISSSQDSISQGSSEFKLVPKNKSTQGEQEEKDLKTYLNKKDIIPYIYGDKLTKIGQNGSKLILPDGSTYLIDYFSHQTELRQNKFPKLIQDLKNSNYQDQEYILKEIERMAKQNRNQVLSGQIVPEKLKNLEALNKVSEDLLKDHRILEYKLVDTIVYLMHHEQMLRSNLFSNFRLEEIILKQHLIKSDEIYKQQDNYTSTLCFIITLKYQQQGIWVSNFSGISIEIIKNLSQSYEFRKWKESRKNQEKMQQKLILENSKLQFEPVTSDEEFNENNNQGNAQSLQYQESNHKAIGYIVNNGNFNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XHM0</accession>
    <name>A0A8S1XHM0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1240025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000124">
        <property type="protein sequence ID" value="CAD8200248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XHM0"/>
    <dbReference type="OrthoDB" id="296380at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398:SF0">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="327"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8200248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="603" mass="70237" checksum="975B06302A5F5555" modified="2022-10-12" version="1">MNPSLAQKFKNFCQTIDLYGAYPSLLIFGNQKYSSILGVLFTLLASTLTFAYLVQQVQELVYKQLPQTILSEHLVSETSPFPLQSQNFTLAVSVGNIQSNPLKTINKYFTISVENCQRIRQLNTTTNKIDVINTCVTYPIEACSSENFGTELQKEYFSKIRLGTVQCIKKEILERNPPILQGIISGNIYQYISIKFSACKNSTEKQDCATMDQINEELMNGYYVVHMSDSLIQMSNPENIQQNFIKMQFTSFSISTSKIIYSGFRIIESITDNGVLTNNIVEDQFLVQQYFQESSSNYNQDYLVNHFIIIDNKYTSYQRSYIKLTTILSKIGGLWQLIFIIFGIITKPFIQTEMRINLANKLFRFQSELNDLTQNVKKDNSTNKNLDKYILNSGSKLPNSVFDILKFIFGCHKNRIKSLNLANQRIQENLDIVKIMRIFQEFDNLKQILLTFNQRILFDLIPTPLINGQLDNKMKQNQHSNKPIIIDNTQQQNKNYQDRFIEAYKSFFQIQIMSEMNENIGQKIVKCLDQEMIKVFYEYQSDTESRQFQFEDRKSKNTVDRNYQDFESKLSVSPNNSNIQEVQTVKIFLNNQYNRRKFDNLQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XIK7</accession>
    <name>A0A8S1XIK7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tubulin-tyrosine ligase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1260083</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000126">
        <property type="protein sequence ID" value="CAD8200973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XIK7"/>
    <dbReference type="OrthoDB" id="202825at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_125, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069">
        <property type="entry name" value="TUBULIN TYROSINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8200973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="529" mass="63124" checksum="6A96B8FA9EB98225" modified="2022-10-12" version="1">MNSPKKNAFPTLNNLKIIQSSYGQRINKCTHLVIKKDTKKIIDTRLLSQTSIEQAKQKFIANQERQRQMQKSLPPIMKRKEFHQRSVSVSAQKTPYSKYFYFVGEGNNHQLIKKLLDERSNWVQVKDPKSKKINFKWQETEKGYEYQTINQKEQPIQMLNHFEFHSEISDKFKLAMNFKAYCDKNRLNIYDYIPTTFAIECTNDNLKSELFQFISFYRQNCPQHKLSELDKTIKQYFFSYTTLYDTKVNHYPTLFGNENLWIFKPSNMNQGRGIHVVRSLQDVLDIQSKYQGGYKEKLLEVKRNEQNEIINKVVYLNTLVTEQFVIQKYIEKPFLIDGRKFDIRAYVLMTSNLQMYFYREGQLRLATEKFDAKVQSNYVHLTNNAIQYTHPEYGKSEEGNQYNFEQAQNLFKKDFRKEILVKIKNISYTTFQTVKHKINKHKRKNCFEIFGMDFIIDEQYTPWLIEVNTNPSLEVTSKLLDQLFPRMVNDAFKITIDLVFPQEEPNCNYPLMNYKDDENLWDSMGYLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XJA1</accession>
    <name>A0A8S1XJA1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">UDP-glucose 6-dehydrogenase</fullName>
            <ecNumber evidence="2 6">1.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1270049</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>UDP-alpha-D-glucose + 2 NAD(+) + H2O = UDP-alpha-D-glucuronate + 2 NADH + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:23596"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="1.1.1.22"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.22" evidence="2 6"/>
    <dbReference type="EMBL" id="CAJJDO010000127">
        <property type="protein sequence ID" value="CAD8201261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XJA1"/>
    <dbReference type="OrthoDB" id="5059218at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003979">
        <property type="term" value="F:UDP-glucose 6-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006024">
        <property type="term" value="P:glycosaminoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000193">
        <property type="entry name" value="UDP-glucose 6-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017476">
        <property type="entry name" value="UDP-Glc/GDP-Man"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014027">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014026">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001732">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028357">
        <property type="entry name" value="UDPglc_DH_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028356">
        <property type="entry name" value="UDPglc_DH_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03026">
        <property type="entry name" value="NDP-sugDHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11374:SF3">
        <property type="entry name" value="UDP-GLUCOSE 6-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11374">
        <property type="entry name" value="UDP-GLUCOSE DEHYDROGENASE/UDP-MANNAC DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00984">
        <property type="entry name" value="UDPG_MGDP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03720">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03721">
        <property type="entry name" value="UDPG_MGDP_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500134">
        <property type="entry name" value="UDPglc_DH_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000124">
        <property type="entry name" value="UDPglc_GDPman_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00984">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0520">NAD</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDP-glucose/GDP-mannose dehydrogenase C-terminal" evidence="7">
        <location>
            <begin position="322"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8201261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="434" mass="48966" checksum="4F5A29A75E6FDA9A" modified="2022-10-12" version="1">MQIQKVSCFGAGYVGGPTMAVMASKCPKQTFVVYDINEQQINKWNTKQYPIYEENLNEYINKTIEKNLIFTNDIDLALKDCDIVFLAVNTPSKSYGLGAESQLDISYIDSCLQTIKKYPLTRKLILVEKSTVPIKTCDYINAVLKNQNICVLSNPEFLAEGTAIQDLLYPDRVIIGGSLESSQQLASLYEQWVPKDKIIFTNIYSAELSKIVANSFLAQRVSSINSISIICDKIGADVNQISQCIGSDSRIGNKFLKTSVGFGGSCFKKDLLCLIYMCESLQLEEVAQYWRQVYLLNEYQKQRFINLIITSMFNCLRNKVIVILGVTFKAKTNDTRESAALMIIKKLQEEQAILRIYDPQGKIDNVEQCQSLDGIFQGASAIVIITEWEEFSKIDYVQAFNQMAKPSYCFDGRNLLSGDLMKSIGFLYYGLGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XJB0</accession>
    <name>A0A8S1XJB0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1270020</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000127">
        <property type="protein sequence ID" value="CAD8201233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XJB0"/>
    <dbReference type="OrthoDB" id="282417at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8201233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="94" mass="11357" checksum="9147FE28A8F17A78" modified="2022-10-12" version="1">MSVNVQKQSLFKRAFLQTEFKVACYPKIELNTSKEEAWQILKQPQNGFERFALRFISKQYIVPRKPFVLSEVMKQICNEWNSFDQDTKYYYCNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XLB8</accession>
    <name>A0A8S1XLB8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1270063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000127">
        <property type="protein sequence ID" value="CAD8201282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XLB8"/>
    <dbReference type="OrthoDB" id="299768at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="411"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8201282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="721" mass="84453" checksum="9F065E86B8950D4B" modified="2022-10-12" version="1">MSKKQNSQSAPEILQYKVRPQNHYQIEQLVLNDMKKKIINDEMNSIQFYDIIQPDMNEIVTTNPKDLTKKPIKLHKKVKRSDLESNLRSKLVNEPGGKLLLQEIYEKLDEDENTQQVKAVLHKEEQFKGNRQYKPESDDLMVANLDQWWKQVKIKKQQEFEKKQLEIEKEKQVIAQLQKTVSISSFRRGAVDKRTGTTLLPAFPKLMQSQSSDDKSPQTENQNLFQAKVKGSLAKLSNLKKAELLHQDMGLVKQLDKYLQQGSTLTSQELINIEQARQKQSHEEKQTDTLIFDYTVEGLKNSLHTRIKMHLTNFLNANERAVFYEKQTNSILQQINAKLDKQKKIVTKNDHDLETLRMENHEIYNRLRSIEDIQKQLKEELGKVGNNNNSQIQKSQKSSNVDFYMATVGKQNEIESQLETMKDSYKKYKLQIKLNFKQIEKLETENSKLNKNNKELLKSIQGFLLELLKIGLDCRKQGLCWIVKAVWDTNEKLSDDNFPSYLDSKGREFLIQKAKKIIELNDLFIRAHQLFGSYHGKLETSQDNLNTSILSILSKNHFSQIDSEDIKRKTLRDRRMKSSGEINLLLKGLNREEKQSIQGTLTQIFQSKQTLNDIIYTEAQKKILKMFKTEASVILEQSFISEDKEKLIREYQQIQKDIELKEVLFKKFEDLELQRIVKEIEYKKYLQRFSVEPLQVLSALFGYVKAERELIKQGMMHISNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XUV3</accession>
    <name>A0A8S1XUV3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1380115</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000138">
        <property type="protein sequence ID" value="CAD8204827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XUV3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8204827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="63" mass="7489" checksum="513267A6F79CB6E1" modified="2022-10-12" version="1">MKLTSNVKLQINQIIRSRRTTFIKNQLFLFLQKSSNNLQNYKALRKNLQKVFGISSFMRKAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XV38</accession>
    <name>A0A8S1XV38_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1370096</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000137">
        <property type="protein sequence ID" value="CAD8204472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XV38"/>
    <dbReference type="OrthoDB" id="309700at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8204472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1080" mass="130169" checksum="87C084FBB906DA8B" modified="2022-10-12" version="1">MSQIQILNSAEFCREQIVLIEDHTIYLFRLTESFNFLNSETNNNQIFYKVLFSKSCHLEYYILIQAIDKHKSLYEKYKEIQQSIDQKVIQIYSAFNYLFFQYYNKVQIFWLGDQTFEIICFPQSIVFIDQYNLIYIIDKQNQLISFYKINKLFNYLQPTKRYVYEIPKSYQKIYTSLCYEIVYEDIKTKSNIINYEFKNECQSKEEIIVKKDNYNFPNYQDFLNLGEYQNTIKYIIQNVYLKEFCPVIEQYLEENELLYYQSKNLENYAILNSNDTLQVLFCNNQSSLEKDVRNYQVIQFQNGILLIKKKLQNTKVIYYSIGLNQQHQIYINSQIQEILQFKQYVILQTENQEDYLFLDILNARQCEISNKLKNMLNLLNKDQIQSKMIRFYFDQFPLFQIYQINKQLVIEKADQIYTYKINDISVIYTGESMKLSHNEILIYKYCVIAIHNKYNIIKQYILDDSLKLLENYQIEDYDLIRPIVFQNSKEFLVIALIQQDQNYLFIFQIYQPLKLIKILKIGKLKFHLMNNLLYYYNKDQEIQIFNVKYITISYMNLVESQKQIVLNEKIPILFKHQTEENPPDQLYLEIKVNNYCRKLNSQQNEQIIYVEEKEMIRIENIDMFYGPIDQLSLRNNPKLQLVGPFLQQQEIEECLIQQEVCYTNMVFEQTVNDEQQTLFYSLRYYNKYKILPFYSIKNLKDNEIITKNAFIFRNNLVLYFSEIKKLVFADVLHFNRTQLNQQYDKLELQGFIKDDFKQAQFYQSNSLILIKTNQSEKLLKVTQNQIAEIEINFSLSNILFIENSANQYIEMKIINKLDECYFLFNIFQLKSTDLLIKDSIKIEQDKIESILFTQIDIEIYQLQEYFKNQLLESYLIENEIEIKILQIGLSFSVISKITINLNKQNNIYDTIYDIQRIIRHPKSQELLNFEYYDNNILILSNSNNKKSFLYDLRQIKQFYDYIQKIDDNLLKLYPLNTTHYLFYFKEINQFIIGEIGFEIQRLEDLDDVETCIIKASNQVSQSQFTLIIKRVMFLSKETKIMTTSAILLGSFYLLRRNKLKQQEIQNQISINSQISQLNNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XXK0</accession>
    <name>A0A8S1XXK0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1410140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000141">
        <property type="protein sequence ID" value="CAD8205757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XXK0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="139"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8205757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="244" mass="29360" checksum="9519F0955764E35E" modified="2022-10-12" version="1">MAEIQEKILKFKCLLSNNQIDQKYLIQQNKIIYLLGKEEEEEGEEYVESNQQLELFDKLNQKYIDFQNNDEWKIKQVLIFTIIYVSSNSFTDTIISFCQKALIQLWVVEKYQRVRNLLKNYNQINLWMQILQKDWQIQHDRVAGEMQQMLRRIDELQEQISREANLNKPDLQLKEMDETTLITQTKRFVRVEGKINQMKEQVNSMGNDIKFMSRKSKMESFEGSSRKKCQIHLCAMKNSRERQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XXS4</accession>
    <name>A0A8S1XXS4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1400062</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000140">
        <property type="protein sequence ID" value="CAD8205258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XXS4"/>
    <dbReference type="OrthoDB" id="296733at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8205258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="597" mass="70966" checksum="65C69FDA4182A8EC" modified="2022-10-12" version="1">MEFFEQFNKKEITEEQFVEQLQTLTTKIQKKSTLTSEQILQVNQQIDKIDDLCFNSNVELFIKFALYLSNRGVYQLLYQLSWDKILFQKDNTDANLLGLYIIKRVYHSENHKVLILFLNDILRQQKTIEKKFVLLEIFVQILPKIEKREIHLAVIFPVILIVINEGLQKYLKYKQQQIQNDLNEDNKFTKLYMNNFQNWTVQFFKRIFDSIKKFPEIKTQSIAIRELYSIDTKYDYFDNQLSRKPEDPKLIVKHYILLFLSDLLQGILEIEQSMKDSQLDPDVIQTLLTQTNQMIYDIHDNKLEIIENYINWIRFSIQFDQELPQQYQHYSNEQTYTPAFICYLTKELLTSNSLESILTVDYKLKILIGVLYEQKKLTVLKCDIQSKLIELIVKYLNLRDVSIKLKTANHFITPFDIIIMLIMEKAGSGLVDEQPYISAWKQAQSSFIPEVWTRILEQCINKSQNYVLLSYLVNNIKSSYSQLDNKILIKNYDILIQAIFKKETGNIDAKVLLYDSATLLIQQLNLKKVDLHNIFEQKWKPLLKQYQKDLQQSIDYLETRKKEGDEQKLKDDQVFQAILGQERNLKIAYYNLNKVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XXY0</accession>
    <name>A0A8S1XXY0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPENT_87.1.T1430113</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="431720at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008331">
        <property type="term" value="F:high voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000082">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000087">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000093">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000117">
        <property type="entry name" value="Voltage-gated Ca2+ channel, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000162">
        <property type="entry name" value="Voltage-gated Ca2+ channel, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000166">
        <property type="entry name" value="Voltage-gated Ca2+ channel, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050599">
        <property type="entry name" value="VDCC_alpha-1_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628:SF7">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="3" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="462"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="500"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="531"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="583"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="687"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1105"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1130"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1225"/>
            <end position="1247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1296"/>
            <end position="1322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1445"/>
            <end position="1467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1487"/>
            <end position="1507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1575"/>
            <end position="1597"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1677"/>
            <end position="1703"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1756"/>
            <end position="1777"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1789"/>
            <end position="1810"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1822"/>
            <end position="1843"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1855"/>
            <end position="1874"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1886"/>
            <end position="1908"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1928"/>
            <end position="1945"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1981"/>
            <end position="2003"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="15">
        <location>
            <begin position="460"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="15">
        <location>
            <begin position="1104"/>
            <end position="1331"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="15">
        <location>
            <begin position="1453"/>
            <end position="1709"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="15">
        <location>
            <begin position="1757"/>
            <end position="2009"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="38"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="43"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8206266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2380" mass="280965" checksum="C2F54E564533C239" modified="2022-10-12" version="1">MFPNIIQQDRRTSIMDDQLIIHNKNIPHTPELFSRRIQKRQSSKIGSQSQIVRNQRSKLSIESHSQQRKATLSSQKNRQNNLFQEYQDLFLSESSDSNSSSAGTSKITQVQQKGAVSITNYDKVIDEQMEFIHKTLHQQLSSISKQKATSFLKSSQNSQMKQTIKSMKSIKQLSQYSKKSSFQSKLNYSTKFNRERSISTSKNVNSDTQELKSLVKILDQAGIPEQEQNQYKQIIEELEKQCAEIVPNGRNKIKQKVRKTFVQLFNQYYKEDNQLKGMDGDQMSVYSVAQNESIFFGNRGHEPQSIQNNPSNINMTSILDNVFTKTQNHGLMSDRMKILMDNFKLELSNDDSNFIAQSEQSSEIEADEDSEEEYLEQQFTQTIRDPTSQQDHQIIKYERKYKRDPIYDPYPLIWQSKEDYQWIIKDYQFYWDNYPLVYLRKTIVFIIRIITNIANKIINHRIFTIMVLISIIFNIMVFIESQSDNLSTEVQMNLRQQKQIILFFFIVENILKLIGQGVIDFFWDLQNVFDIIILILFSFHYYYPTLMIIDFSTARLLKILSVISVFSKRLQIMLLALKHSLRFLLEALIIVIIFSYFFALFGMHLFQGLFLHKCYYAESGIQTNILCGYNLQCEDQSMICFKSITNPNVPTNFDDIFYSYIEVVRLMIFNEWTEPIYLAMLTFHDFAIIYFIFLIFIIAIFGANLIIAVLKIYYSQTLLKYQHEDIQKLYANAEIILNLRIIKNYSIDFDWKTNSKKDWVRKKPHDVDDQTTYAKTILQNNNNQKIINYKHKYLSARQTKEQEEIKNRKRGIKSNISKLENLIDSFKLEKLLLFEQNKEQFMQKLYQNDAFDKEYLKLFYIQTFKQVPLYSWRIVINKYFQYNSTSESDIFSFQIKNEQLQKKREREFEFIRKSILNHCYRIKKSDYSFIKLDLKNKYSSNHLKSQFPIKQKSKQKQLLNIEKNKTSEKALIIENNINNQEVINIIPNNQMNNRTIAQVDKTFDSEFIQQMINRQIPENQEVIQNFEELYVKYRLDECKQGVVYKNNWSGNCVLQSKQKISQTIKIFHQLNNIDIYQWKRPNLQCVWNLLSKNLNILLRNRSAHIFFDLIVLINIILLSMIGYVDQNIIGQLNDCFVFILLGELFLKLVFQGINRFCSRANNFIDTFILTCSIIQSLYVYQNSGNGNIYLDLLNSSQALLIYRIIKYNNFALKIANITKKSLPSFLNLILLMITLIFCFAFIGMNLYKGKFPIETDFGLQQSFDDLAAAFLTVFVRAANEDWFGMVMLGSQYSSKIGTMFFSVISVYVLNLMTIGFILAIILDSFSSYENEDENDSGENVADLQFSFLEGNDNINEVTLNSNKVLTSHHENSEMNSNGNIIQDIEKQTIKNNNFFDLASRIKLLLEQLFAKFQFYTNNESENSLYIFSQSNYFRKLCYRCINYQLYINLIQVTFFIVLMNMCVYTYFDYENKIQKNLVVQISNYVELATNILLLIDSTLKIISFGFMKDKGSFTSEFWRLIDFVYQLCYISNFVVKYDAFQYLKVLKYTRPFRFLYLFEQLKYVNSAISKSIVDLMNILFVQIMVWLIFAIFGVMLYKDKMSYCEYPLNFGINKQECIEQGQEWINNLYNFDNMGNAMLALYRMTACDDWVYIMQICLNSRAEDQGPILYGNRWVTLIYFMLFILVGVLFFLGFFAGILFINFQTYKTQLQKQILTNDQQLFANITKIIQKEVPNYSDPPKSFIRRLASNIIKQILYERMILLTIFINTGILTFFYDEASLDLLQTLEYIYHTLSCFLILDTCLKILAFGIRRYWGYIWREIEFILSLIALIDFILYLTINWSQYYFKSTIDNQYFLILRLAFACRNLRTLLIIQQFKGLTRLLRILNFSASFLLQILCFFFSILLFYGLIGCEYFGMIEKGIYVTDYMNFTNIGKALLILFKICTKNNWIRIMIDVSERNQYCTTETSNQCGVKWIYANIFFYTFVLISNFVAFNLFITALVDQFEKFFNSQNSVLQTYIENIDPFRTTWCKYSTETKGAQMHSKHLAHFLLELGPPLGSAQDDNIWDAAKSASNFKIKADQNGYIHFQEVLYETIRYAYRDQIFRTGHSEGIKVIKQIDKDTRFRLHFKRLDILDRRYPIYEMMHLKGNFNILQEYLFLLMIFRTFKAYSLKTIGRIQEAIMSRDNSNYPKELIENSSQSEINDDIEKDKKIMESQHQISLNSEDVETYQIQVSKYIESHKANKKLKFLDQNSNNHSEGLILFMPDSQKNDCIQMAPFKHKSTIKKKQEYNFVDFEAIEQKQLHNPMLDSSHLNEISKGALTDSLINDEYKINQINYSMISPHRDRFSINSINESVGTENHHFFYGKPKMKYCQQVAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y1R0</accession>
    <name>A0A8S1Y1R0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mitochondrial import inner membrane translocase subunit TIM22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1490061</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Essential core component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. In the TIM22 complex, it constitutes the voltage-activated and signal-gated channel. Forms a twin-pore translocase that uses the membrane potential as external driving force in 2 voltage-dependent steps.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the TIM22 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion inner membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Tim17/Tim22/Tim23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000149">
        <property type="protein sequence ID" value="CAD8207971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y1R0"/>
    <dbReference type="OrthoDB" id="305651at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042721">
        <property type="term" value="C:TIM22 mitochondrial import inner membrane insertion complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039175">
        <property type="entry name" value="TIM22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14110">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14110:SF0">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8207971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="146" mass="16311" checksum="AC881BD8FF5B0B59" modified="2022-10-12" version="1">MTQEPNPFKHVTFMDTNCAAKAGMVTVAGGAMGFLMALFMNAVEMRDMDYGRTKQSTRYVLRRDINKMFGMAKGFAIFGAFYSIFECQLEKLRIRDDATNSFLSCMFSSMVLAAESVGWKGLMMSGLGGGMFGGVMYYVQIKFMNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y2W4</accession>
    <name>A0A8S1Y2W4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1500038</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000150">
        <property type="protein sequence ID" value="CAD8208249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y2W4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8208249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="64" mass="7817" checksum="FD031499CE92A162" modified="2022-10-12" version="1">MLPHQNIYLTYQILLYLCPHRNNIWLKLQPKKDSRMVVQNMKQNDKVILKMKKHGNLLRINYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y518</accession>
    <name>A0A8S1Y518_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ankyrin repeat domain-containing protein 54</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1450094</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Plays an important role in regulating intracellular signaling events associated with erythroid terminal differentiation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000145">
        <property type="protein sequence ID" value="CAD8206864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y518"/>
    <dbReference type="OrthoDB" id="294746at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24197:SF44">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24197">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="761"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="794"/>
            <end position="826"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8206864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="889" mass="104986" checksum="229B981207A771DE" modified="2022-10-12" version="1">MKTKCFSARHYTDNENKEFCNLMSQQQIEERFAIIQKQNSFRQIKRRNTITVRQNKVEDLCILFEKLIKKSNKNKPLKQKYHAICQESGIDFNELRNITRIDDKQDYIIKCCVNQISSQFDNILSKYNINSNTERHSNKNDDQLDLYGWLQVKSQYKSILKRLKFALVDLIQTSNNFKKVNNFLSSKSKINSQQVSFIHTTNNIDSKEQSTQYTSRIQVNTDKKLILNVPELQYSPPELFFEWNKAIEDGYISTGKNMHLMGYETIKKQLANGIHSKKRDKILNKCQQTGNYITEQELQYIASTSFLINKCIDRDFIQRAINCLEDNVKHQIELNISEQILQNFYFIKENQVKIRNKYFEELNKKKRQQITENTKNYERYQHFSKDSLQIRVIKGKFVQYLKEAQKKKFLKEAFDHKKQSALRKLKNSGNPLANIYVQKFKEIMLGIVRKYRERKGTEKIKQQNFLERNTSKQTFKPLFIKGQDEFQLEQVKHIFHPPSKLLTLDPEEKFQQYQKEKIIKWSKSYRLGIRNYNISQNEFMKFYSGGPQTSRQFQSQKLKGLNDHQELELPSQQQWITDEIEVQAANKIKMAIKRFLYKKKFFKVLEKKEQLKSIDIEQRIQKCMKAKRFFEELHKELNNQQNERLKDITQHSPPKLPMDKVVFIPGSTPTSPQINRLSKHKTTEISLVSSRIIKQSDLQKRETNEKQRQKLLNKFVRTRLDGEAKIKNRKLIQAAKMKNLAIAEAAGFHYSKADTELYDQNGNTPLYYSAKYGDEQMTKFLLKIGADVNQVCSNGNTPVHMAFLSGNQQIIIEFINRNGNLNILNNDNYTPLAFGTQELLQSLNLIHCVATVKQDNIKRIDNQHLLHRKIKSETYELNDELILEIKKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y519</accession>
    <name>A0A8S1Y519_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TLC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1510108</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000151">
        <property type="protein sequence ID" value="CAD8208650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y519"/>
    <dbReference type="OrthoDB" id="284464at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042512">
        <property type="entry name" value="TLCD5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31898:SF1">
        <property type="entry name" value="TLC DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31898">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 136"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="16"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="7">
        <location>
            <begin position="43"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8208650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="262" mass="31144" checksum="9E22E811D4DA6A09" modified="2022-10-12" version="1">MQQWDLVNESNDQLRMFSYCFIVWSALFILCYDRIKLNNLSSKKQIDVYNRLVSILHGQFTFWGSLIIILSQTPYELQEMNSSEMQFVMIVSAGYFAYDVIICTYFDLYDYWLIFHHIISLMAFGESILNKKYGHIIIFGMFISEISNLPMHLRHILGCFGLRQTKIYESIELLYFSLFLIFRGILSPVLLIRTYEDLHAPLLIKISASGLLLYSVYYIIEMIKITQRKIKSYRDRKRRNLTMYWFVQNPVLPSQKYVDSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y701</accession>
    <name>A0A8S1Y701_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1520023</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000152">
        <property type="protein sequence ID" value="CAD8208767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y701"/>
    <dbReference type="OrthoDB" id="10252171at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="119"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8208767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="447" mass="52258" checksum="27C56B4EB3096E62" modified="2022-10-12" version="1">MLQTIGERPQIQPPINYGTVKYSFKCIRRHLFKDKIYYVHALPNMITIGDHINDPFPKYTLELNLKNIVYWKQEHGQNRAFGIKYKKNVKYFEATSEQLQKFRQEISCLIGFHSFDDHYKIDELIGKGSFSSVYKIFRINDKKVFAMKYVSSKQTNKKENMQLVENEIGILKSFNNESILKIYEVYRVEEYHYGIIVEYLDGIALSTLIEQFKKNQNILKESDIKIILQNLLMALAVIHQEQVIHRDIKPQNIMISQQHYNCVKIIDFGLSIKNQLQYNRCGTPGYMAPEIVNMRKDQQKAWTSLCDIFSLGVVFFKLLSKGISCFQGQTSDQVLANNKNCQIDWSIVQQHNYSKNCISLLKAMLAKDPEERITAYQALQHPFFGDVIQTMYTDYVGHSINLKSKQILNPTTEKQNLDSQQSVEDKQIQKQQVYNRDIKPSILSRQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YAA2</accession>
    <name>A0A8S1YAA2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Inhibitor of apoptosis-promoting Bax1 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1600008</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000160">
        <property type="protein sequence ID" value="CAD8210580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YAA2"/>
    <dbReference type="OrthoDB" id="7933078at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006214">
        <property type="entry name" value="Bax_inhibitor_1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291">
        <property type="entry name" value="BAX INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291:SF47">
        <property type="entry name" value="TRANSMEMBRANE BAX INHIBITOR MOTIF CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01027">
        <property type="entry name" value="Bax1-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="122"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="191"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="314"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8210580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="341" mass="39290" checksum="644415B119E28C0A" modified="2022-10-12" version="1">MSNPYQQSNTQYPQQNQPQYPQQYPQQYSQQYSQQQQPYPQQQQQQYPPQQQYYQPQQPNYSYQQVQPTIQKETYYQQTQPQNNVSSPVDDQQKNPLYEYDLGESFLGNVGFDQRKKFIVKVYSLLTIQLLVTFVMVAIACFSQGFRDLLINPSSYNATPFYWSMFAVSFITEIAIFCCKKVARKVPNNYIALTIFTISFSFVVAGSCAVCEDAFSNGGTLILIAALMTFAVTASLTVYACRTKTDFTMAGGALFIMSAIMFILFIFAIFYFNIILWLLLCSLSVVLYGFYLIYDTQLIIGGKSHQLSIDDYVIATMFIYIDIIILFLRILQILMILFGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YBY0</accession>
    <name>A0A8S1YBY0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1600077</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000160">
        <property type="protein sequence ID" value="CAD8210718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YBY0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8210718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="375" mass="41677" checksum="49ACC77B3126BBC4" modified="2022-10-12" version="1">MFLVWFMNRSWNYINLNENLNNFNILNCLLIKLNKKFGTANSPDGPKATKLALTGDIAFVSGSTDVKINLKYSNLDLINDDTNSSITQVDGKTTTTPCLDLKLWKFTSNSYSDPVEVTDLPITPDFQKQWRYYSFNIPKADLAKRLLKTQNTNQFIYKGFYAIAYYAAGTDQVYYTFYFEFSVTVDRATGAAVDTSFKPLSQKATVVCAPVLKWCTDLTCTSFATPDLHLNDQFVLQQVVTTAGMNYYLTGTEVWYTGNGLNKKATITSVNNNTKGQAIIQLRAEIAWSSVTIRVTSTLSDTQAGGRRLLVQTTYDPVTGETDEITCIKAEGKEICPKCEEECSAQGFAEDGCPVCSDATSNGYILAGFITLLMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YDK2</accession>
    <name>A0A8S1YDK2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1660102</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000166">
        <property type="protein sequence ID" value="CAD8212135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YDK2"/>
    <dbReference type="OrthoDB" id="273181at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001070">
        <property type="term" value="F:starch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002044">
        <property type="entry name" value="CBM20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052832">
        <property type="entry name" value="Starch-Glucan_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46642">
        <property type="entry name" value="DUAL SPECIFICITY PHOSPHATASE, SUBGROUP, CATALYTIC DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46642:SF3">
        <property type="entry name" value="PHOSPHOGLUCAN PHOSPHATASE DSP4, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00686">
        <property type="entry name" value="CBM_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01065">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="1">
        <location>
            <begin position="187"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="2">
        <location>
            <begin position="261"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8212135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="338" mass="40195" checksum="0C65383AF2002737" modified="2022-10-12" version="1">MISISIQRHVNYGEVILIVGDCHQVQWNVQNGIQMEWNPGDIWTTRIECCCLPMNYRYVIVKQNNRQIVEWEDGISRVLNSKHDIEDEWSHIKIRIKMNSYNDSKMFVNLFSEKGRSKIRLDQVVTDQNEYEYRLELQIPSKSINQYAYKYQQDQKWERNGVRVFTKHPIINNVIEIVDSDVEFTLNYNQILENIYVGSFLYSDEIHILQKLGVDAIVNLQTIEDLINKDLQEDYFDYIRQSCESYQITYSHFPIRDCNKRSFLKKGMQAHQILKKLIQEGKCVYVHCTDGIQRSIQTIILYLVLDLNYSLEDAIILVKEIRKRSKPIREVLQQLLEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YI20</accession>
    <name>A0A8S1YI20_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1460120</name>
        <name evidence="2" type="ORF">PPENT_87.1.T1740027</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000146">
        <property type="protein sequence ID" value="CAD8207237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000174">
        <property type="protein sequence ID" value="CAD8213133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YI20"/>
    <dbReference type="OrthoDB" id="291213at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090560">
        <property type="term" value="F:2-(3-amino-3-carboxypropyl)histidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016435">
        <property type="entry name" value="DPH1/DPH2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00322">
        <property type="entry name" value="diphth2_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01866">
        <property type="entry name" value="Diphthamide_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8207237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8213133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="69" mass="8006" checksum="D79400A14741C763" modified="2022-10-12" version="1">MPLIIKGTTQASECKCFGILIITLLLNILKCIQSMQIDFQKQSKKYFTFSMNRLNEAKLENFPEIEAYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YL06</accession>
    <name>A0A8S1YL06_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">H-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1650017</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000165">
        <property type="protein sequence ID" value="CAD8211744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="300123at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019019">
        <property type="entry name" value="H-type_lectin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09458">
        <property type="entry name" value="H_lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="328"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="383"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="H-type lectin" evidence="2">
        <location>
            <begin position="44"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="H-type lectin" evidence="2">
        <location>
            <begin position="428"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8211744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="808" mass="94310" checksum="D511F86F23819732" modified="2022-10-12" version="1">MLIITFLIISICFATITYDSNYFLEWNYDIVPSFTCWGGRSFDKTISFSDSFANIPKVFITYEMIDLHWSQDYNTVGFNFSITDITLTNFAMHMYCQYERNYRFRIRWFAIDDQRIDVISQFNLIPPQNCSFYYANINVEKHFITITSFSATGPTDIQLFAYFTPPNIVTLYIPYMIGQSENLKSIGFQLILGIDEAFTKSQAILTVSNYDSLVYDPIQTNQVELYPFSGLAYLDEHLFQLVVVINDNGVGTKYIIRDWGAGYTANYHQKVRMPKSQTKLFQPILCNFIRFSQKLEYRGLPRPLFSIEFTVDSQVYTSSGQILISKSIQXLLLLINIISLFEISFRLIHIFKRFMQIYYKAIFFYFFKLTQKKYLISLQIDQFIMLIITFLIISICFATITYDSNYFLEWNYDIVPSFTCWGGRSFDKTISFSDSFANIPKVFITYEMIDLHWSQDYNTVGFNFSITDITLTNFAMHMYCQYERNYRFRIRWFAIDDQRIDVISQFNLIPPQNCSFYYANINVEKHFITITSFSATGPTDIQLFAYFTPPNIVTLYIPYMIGQSENLKSIGFQLILGIDEAFTKSQAILTVSNYDSLVYDPIQTNQVELYPFSGLAYLDEHLFQLVVVINDNGVGTKYIIRDWGAGYTANYHQKVRMPKSQTKLFQPILCNFIRFSQKLEYRGLPRPLFSIEFTVDSQVYTSSGQILISKSIQNIELNINCNCINGKKIKSKFFKCENCPLNKNSHQFQHSCYGAINLLFINARFITQTNAIQQLQIDITPQSCKITQLIFNQQIQTFVIVDIQMIDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RVV0</accession>
    <name>A0A8S1RVV0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0010119</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RVV0"/>
    <dbReference type="OrthoDB" id="10335257at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8131522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="217" mass="26156" checksum="D391DF9F01D37754" modified="2022-10-12" version="1">MKKSLTFDTDSKYNFSQKIHLLRHQRNKDKIKEAILKLENPFDPSQISQALKCLENQFQRRALLDQIILIRKEKLKMQELEKINQMKDILTKVSRKLSLIKNQQTKYTRDQDDFKILQEERNKFDKFQYENLNFQINEDKHQLRRLSSLSFLTKETDKWKVNPQQQIVQQNLFVRRKTQRNASLIVNAVQKKQIYLQSEQNTTNSLIIQNNSLQFQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RWJ4</accession>
    <name>A0A8S1RWJ4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0010555</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RWJ4"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009190">
        <property type="term" value="P:cyclic nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43336:SF3">
        <property type="entry name" value="GUANYLATE CYCLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43336">
        <property type="entry name" value="OXYGEN SENSOR HISTIDINE KINASE RESPONSE REGULATOR DEVS/DOSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="3">
        <location>
            <begin position="667"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8132326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1013" mass="115886" checksum="997A1F079EB25640" modified="2022-10-12" version="1">MQKLTDKQKGNPLVKKDINRDEEIENDGKVRKVRSPSSVADFNELRSNKSPHSIKSVKSARGSNLGINQSGNDEQRKKPPRRLNSTIMARLSKLQNIKQIPAYQKWISQLIEGWTFSIIMAIVTLYALFGDDIRILSVGKTEDDIFFILTIISMSFFSLEIILTSIINPNYILNFYFFLDVISTATMILDIGWITDLWYGEEGDIGNAATIKALGRASRVARKAARVIRIIRLVRLVKLYKHARQQYEKEQQKKILQQILKQNKLISEENQKHNNNNNNNKLIRNSSYPSPPPSGRQHFKSNDDGQELNEQQKLVTESKQPYIGLIQQQQVPQNNQIGVSQMSAQSNQQSNQFQTNVNAVITINEQLKSSQISLTSSENVKESNVGTRLSDLVMRRVITIVLSILISIPVLQLDTYQETINSYDSGIFRIAQFKDNYQIVLSLTKQYVGFHQDEIYPILAVFVLRQNVTENSPKLNDSNFEIFNYSQNHNWFQETNYAIDEYRQSDKQYYAAIDSNDILVTCSVADLVEYNKINAILSIFQTIFVCIVLAASAVLFNKDVNELVIEPIERMMQKIELIAQNPLEAVYIEEQEDLIMEQLEKDEDDEKIKQKYIERKMETYILQRLIMKVGALLAVGFGEAGSEIIAENIKKGGSVDPMLPGKKIMAIFGFCDIRNFTDATEVLQQDVMVFVNEIAEIVHSTVDSYGGSANKNIGDAFLLVWKYFPMEYHPDPQQPSKLVVRSEHHIKQKGDMAVLSFLKIITAISISKKLEKYKKHAGLNARMKDYSVKMGFGLHMGWGIEGAIGSSFKIDASYLSPNVNLASRLEAATKQFGSVILISGILKQNLTEQCQKQLRLIDIVTVKGSIEPVEIHTIDMSIKNLLAKTKDLQDKFDMSKMNQQEQKQFRVLNRFKRNQLQKDVSKDKINVADQFNTDEEIMYAREPYTKEFYQAWQEGFQFYIKGQWDLAQSLFSKTLYMVHDHKDGPSNTLLEVIHSHGGKAPHDWKGYRELTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SAM3</accession>
    <name>A0A8S1SAM3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0050161</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="300641at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332:SF175">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035775088" description="EGF-like domain-containing protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="3608"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3343"/>
            <end position="3363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3470"/>
            <end position="3489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3495"/>
            <end position="3512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3533"/>
            <end position="3553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3559"/>
            <end position="3581"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="3025"/>
            <end position="3036"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8136460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="3608" mass="419782" checksum="CDBEEDFC3BEFDC3D" modified="2022-10-12" version="1">MINLISITLLFWLAATEYKLILQGPASKKMDCKKLDKNDNGFKGSQFFYEKLSNYGQNTNLDKITFRLWFMVYQKMRFPGKQILFAFLDGYDTDSYINLMVYFNLQSSNYTLYVINEKMNSELMNILIDQQNDFFIGYWNYLLISIDQSSGDVFLNLKLFSTYNYQLQQFQDSLPSKKLNFRFGVHSRITNEQLYQIIQDYKACVYIANFEYINGWTTMDREIYFQDQIDLKYSLKPFQSEGLNVNSQFINVKLIDQKNIEYSGIIGLNIYKDTRILYNFEEQMDSFTIIFWMKPQKISSFFKLISLTDEYVKLISLGFGVNQNYNFLIFQNKLEIYTGNLNPNQWKHITIGILEISRNQYFKATNSVKMLKVYIDDLMVYLQQIQNFKSYKRLLIGPLLLESRGQEVVDIQDFKIFNGYGIYESSGDCRLFAGLYCAFCKSLTHYCKEQDPLDDNNIYQCPAGYRIENGYCKPNTIALCLRQIGGSCIECEQNTILIQGECQPQTQLNRVSPYPCQGLNVVLCQITNIVKNSELTSLQRARLCNSNLYNIAKFECTDNQVASCKYSQYSGLCFECKASFYLTELKVCVSTCFKNKQDRFNYINKCVQDCPYKYTFNHPCPTTGGKEPDLGCSLTPDCQADEIDLGYTCLSISQQEIGKSVGCRSILMSQFAPNQPLTCHPSCKYCFGTTDRQCLGCYEGIYYTPYLGMCLQNCSDNRENIFIYHNTYIWKCELKCPSNLLTQDNMCVQSCLDGYVVFNGICKSISIVDEYFLSSEIVEIEGINQIIQSWNYCPLLCNTCQNNQICNSCINKYPFENNVCKISCFPRFSLKTNNLSTCKDQCDPNELTFWNDNLYGYQIAECFLKKCGEIEADQIYQSYLHQNDNKKCVYPCDDGYYGNSQSILCKPCIVSCATCIDKSHICTSCKPLLFLKGSTCLTSCGQSFKNYMNWQCEVECSSGYTIKDYTNNLYACVENCGQLYATYLYVWRGQCYENIPDISVFCINYQCEDCHSSCLQCQGPNTNDCLSCYQNSYLLNNECVQDCGILKHDQINWKCVEECPIESLQSIGISLILNKQITICGLTCLLETFQFRNECYAQQPIETFCLQRLDYQFCELCSSLCTICSSSSSSDCQQCISTAFQYGTTCYMECPDEAPLKDTINKQCVVQCPTGFSEDGYCVEFCQPISYIYQLKNACYTVGCPIGTFNEIGSKICQDCYIGCATCYGSNQSECLSCIVGYFIDQETYCSDSCLIEPNIIEDQINRRCVQFCPIDSFLQKLINGRYGCKETCPEYYYSNICVSYCPSQTYQDGYACISCASPCSICFGSAITQCTQCDVGYYLYETTCYQICPFENPYANIQDSKCVQTCPNFLYLPKMLCFDSCPFFLYRYEIDNQKQCVDNCLSKSYLLDQDCYPCDSICKECYGPVNGNCLECELPYFLNGQTCEVTCPSFYDLTVHQCKAACPVNLVIQNIYCQTQCNQDYLQYGQNCLLQCPQFTYLNNNTCQNCNAYCLTCFGPNFSQCYSCIDGFYLNGQSCQQICDNYYDQDNKTCIDNCGSKYLIKDLKQCVLQCPIGYISCEQECLISIQDGFYLNNGQCYRCDQKCTKCTSSTSCSECSQNFYLELDSCVNQCINKYLYMDPISRSCVSRCPIGLYHQESFNRRFCMLDCTFKLDDQCVQSCPIGFYNDNSFCKLCPSECTECQSTTFCLKCQIGLYLENNWCYPVCNYKKTDRKNQICVDECNLDLFEYENQCVESCPSNPIYFYHKQQCVQSCPTSTFQQEQYCIDCHIQCSSCFGSNNDECYSCSLGYYLDNNQICTQTCPFLYDLINQKCVFQCQKDQYLEQNQCVEKCNHFLYDKQCVDYCPSLTYQQNTQCFNCSNNCLECNSFGCIKCKIGSYLNDGICDSYCQIMYNDVKQECVEICTNQEYQYLDHCYSQCPIDTYEYKQICLLDCPIKTVQYNNFCYDCPERCDICLSEYECLKCNNLYFLFNGQCVLNCPLQLPYEDTVNRICVSMCPPNTFIRNYTCLSTCNLITHQNMCLEQCPKGYYGNNICKHCKFECLACNDLDYCTECDINYYLEDNQCDNQCTNIKDLKTKKCVEICDSFLYKNICYQKCPIDTFQFESICVIKCPDGYYGSKDFICEQCPYQCFSCQNQFVCIQCKIGYYLFQNQCLESCLDQSFSNPINNKCSNECPPTTFIYQNQCLFQCPNEYLHDIQNYKCVLQCDDQQYKEKNNCLACSIECNKCYTYGNNNCINCANLYNLNENGYCLGECPKGYFKNEQNCQKCLHKCQTCINQIQCVKCRGTNRNSIDCSCQKGYYDDELQENCQKCPCEECISYQNCQICKNNLQIPKCNCDRKLNDDWCITCEVAKVKIYYSDDLNEIVVYFGYLVSVNLINPFQPSDCSLWFDNSIIFGQNSKCYLAWNRYSLHIQLSPYSTINIGDELQFKQSFYRDVDQGICSQQYIKQFIENKLLEPNRFIKPYIIFDVPQYVSSCKQIEIKQILIQGTAQKIQTVISWQLHSIDNEDYYLSMDSFLATQKNEIIIPINTLQPNLKYTITAKYINMFQRVNYTTFTFNTIPIQTPYIYLTYNPLTVNIYIFDCHITFSDILSDQNVQIDILDSLISDQKYISIKQSIHSIRKIPLDEILLPKQVPLLFIAITQSYIIQEVLILKSKPIEISFLQKNRFIGQDDQINARAYDKNIQDIILSTKDIHYQWLCTNLHDLQPCKTEENKILEFAQRRIINIQANSQNSTFVFYVKASKDNRWTIKEQLIVLLDLNIQEEFVINRDQPTDLINLNSEVTILLKKSQTYAFIIQNHKVLQYIKISNASLKFRIAEITQDSTHPIFIYLVPGTDSFGFKINEIPNIFTFSIEPKIGQSMDYFNYTIDNLNSDQIASIYYYVDYYTFKNDIDAKSLLNGIPLIFNTQITSGQFQIPSGISDDPIWIVCQIESNQGALIFQWTQIIVQRRNYEMHTLFEDLGNLVNFSSLQSIHTMISLINQEDKQVCLKQCSGVGTCVDQKCRCPPGYYFNDCSGDKTQFENSNDLIFRSMQALIVKDIIDNEQFKLYSQAILYFQQYKDIISNTSIDKCLKVLMNYITNLNNRMEQINQYSINMQYQQTNQFSYSQIDTRQILSIQDISNAILSTEFIWNKALSISNSSIYQIMHHLNDFFIHIIDLSFLAILPNEKFEISMSTVNLIIQRSNNLQNLSSHRFLQQDVNTDYYDIVQAIYISNFYSYDGYYPYPNQKYPLYDYKIRQQNRYQNIKISKPITYKFEIQNDTTNLVCISRNAKTYEWTKDNCYLSIINNSYFCICNMIEPVTICNDYQFIYQGTPPFYLKLPNFIFIFYFFQFILLALVIYKSQYGNKQQIAKSNKFGKVLKLNKFESVRMMFNNNLPSPISPTSPTSPISPNRIYPENDCIKETVQNIDQKVINKFNFNYFWNYHFLTSIIFQSKFYFSRYQRVQFIILKWNICFIVGEILSIFYFQYDIQIYILLASVIICRINQEFWKKQFKYYEYMKSILTLNLIKIILFIFTTSTFIIGVYLYFWISDQTDFILSYITTNLIDLLFLDIIIFFANKYLGQTKKKLIKKKIQEFKLAMQGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SAZ6</accession>
    <name>A0A8S1SAZ6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phospho-2-dehydro-3-deoxyheptonate aldolase</fullName>
            <ecNumber evidence="4">2.5.1.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0060065</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2 4">
            <text>D-erythrose 4-phosphate + phosphoenolpyruvate + H2O = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14717"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="2.5.1.54"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="3">
            <name>Cd(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48775"/>
        </cofactor>
        <text evidence="3">Binds 1 divalent cation per subunit. The enzyme is active with manganese, cobalt or cadmium ions.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class-II DAHP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.54" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SAZ6"/>
    <dbReference type="OrthoDB" id="2338at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003849">
        <property type="term" value="F:3-deoxy-7-phosphoheptulonate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002480">
        <property type="entry name" value="DAHP_synth_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21337:SF0">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21337">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE 1, 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01474">
        <property type="entry name" value="DAHP_synth_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0104">Cadmium</keyword>
    <keyword evidence="3" id="KW-0170">Cobalt</keyword>
    <keyword evidence="3" id="KW-0464">Manganese</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00047508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR602480-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8137386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="381" mass="44724" checksum="7D602ADDD8ED92D6" modified="2022-10-12" version="1">MNQDKEWSQDSWKNYSALQLPIYQDAQLYQDTLQKIKNLPEILNFEDINVFKSQMIKVSKGEKFVLQLGDCAEVFDECTDEHWKEKFSFYDRMGQILDAIVIGRTCGQFAKPRSQLLEKDGTLNYRGDLINSLSRDDRDPDPSRLLLGAHYTKKGIESLKQYEGQQIWVSHECLHLGYESAFVKQNQDKQYYLTNTHFPWIGDRTRLHDHAHSNFIKGIYNPVGIKIGQTINKTEFINVFKLINPKNEDGRAFAIIRLGKNKLDKLKDIIHWKQEEKLNISFFLDPMHGNNIDKSGHKIRKIEDIMYEIQQFFTILEQQNEIPAGLHLECTPYDVTECIENDEDINPKKYTTACDPRLNFRQTRKIIIFVNTLLKKLRNKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SCX2</accession>
    <name>A0A8S1SCX2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0050238</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SCX2"/>
    <dbReference type="OrthoDB" id="10336351at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035941567" evidence="1">
        <location>
            <begin position="21"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8136614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="95" mass="11588" checksum="F2CFAC365055AEBE" modified="2022-10-12" version="1">MSKSLLIIFVLLISLKTIAAEEIDLSLIRVPNYITQEDDIQLYKIRKQQLEFQKSKYMKMSKEERKKVEQQSLAFQKWENDLKRKYQYKKRQDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SHG7</accession>
    <name>A0A8S1SHG7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0070469</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8139096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="310091at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000793">
        <property type="term" value="C:condensed chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43941">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43941:SF1">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="33"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="130"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="233"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="320"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="402"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1084"/>
            <end position="1230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8139096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1284" mass="154901" checksum="365064BFBA610A55" modified="2022-10-12" version="1">MYKQNPNEKEPYSLFSPSINLNKQTDNTQSGLFNRDQQSVQQMREQIRQLKMQLSKTNLEKETAIQENNKQIRELERVQIYVTQLEARIKMLEESLIAQQTEAERKLTLQKQHFEYLKQSTIERQQTGMQRDFNLEKNDLEIQIKLLKQRIVELEEQLQKVTSIDHMSGYEDLQRQIDSLTLQLMSQKGQERQFFEKMQDLQKQLQISTSKYDQLVHSSKQEIEEYIEKITLISNQLKENQEYTEQLEEEKMTQQLQLSHQVFLAYNEIERLNIKILKINKKMKFIEESHVQQLEDRQHQFENQFNLRQQNLQKGSNELKLSYELKLQNLQLQLQEREEMIEQLKLELKQKQQRIDELTKQLDLERQKIKQQFESFTIQIRDHKNTSEKAYTELQTNSRDQIIKLQQQKQELESILNRIKSELENQKNQNSQLQNQLNQLQLEYEQMRQQYEQQITNLNLEINRLKTQMQQISGKSQQSLDELQYQLEASKQQYSQLLEQQQQLQNSVSKKNELYENEIKQLKQKLTQGANDINNLKNELDKEKEEYNLITQDYQQQFQLLEKRLKDKENELLQLKRTLQQTTESYSEQITQLELQINQLQQQLQQQQSQFTTQLKNSEKEKEKLKQTIKERENEISQLKQTIKTMEENSNTSISQLDIQLSKLQQLYQSLQQESQQQKNQFQKQIQQMTQTINELKERISEMQLEYEQNENNLNENMLKLQNTNKDLQRQIQLFQKQIQEYEIRIKFEENKGSDLNQQLESLQEELEQLKLEIKNQERDKEKLKSQLKDLQLQYEQLLKLKQDLEQKLSIVTQQHDDLTNEYNEFYMNSQQQQEQLQGNIQEKDKQIKNANQQINQLKQRISDLERQVIQMTHEIEERDTKYSELEQNTNMKLQKLNNAIDQSKKQNQEDEKLWKMKLTQLSDQHEERERELQQEKEDLQQKLEFQLNQLKKSKQETEQRLSQLQIKHDQLENSYEDIQREYNDLQDKYVIIQQQIQSLTIEIQLLQKFKLDTNDNELKFQALRDSLESQLKLLQTKYDSLLSSEQHLLEENRKLEEQNNIRIGQLEEFRLKFETQTVSLVDYQTMKQERDELTAKNFSLSQQGLKDKAEVQKMKREIENLKEDLKTKENEVEEAYNFARTRQTTGDKQKNETDMLKIENQKLQNQIKGFEAKIIVQNEEIDKLRRQNEDLQYQLQQQKALVIKLEQQLHQCNSENQKLTQLYDQVKAKYDQAYEGMKNESMFRTRESVMNKVDDGNYVKRVVQTTTLNSRMVASKYQDQEFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SIX6</accession>
    <name>A0A8S1SIX6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutamine-dependent NAD(+) synthetase</fullName>
            <ecNumber evidence="8">6.3.5.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">NAD(+) synthase [glutamine-hydrolyzing]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0070285</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>deamido-NAD(+) + L-glutamine + ATP + H2O = L-glutamate + AMP + diphosphate + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24384"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">In the C-terminal section; belongs to the NAD synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.1" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SIX6"/>
    <dbReference type="OrthoDB" id="2020662at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003952">
        <property type="term" value="F:NAD+ synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07570">
        <property type="entry name" value="GAT_Gln-NAD-synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00553">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000036">
        <property type="entry name" value="Glutamine-dependent NAD(+) synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.110.10:FF:000003">
        <property type="entry name" value="Glutamine-dependent NAD(+) synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02090">
        <property type="entry name" value="NadE_glutamine_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014445">
        <property type="entry name" value="Gln-dep_NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003694">
        <property type="entry name" value="NAD_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090:SF9">
        <property type="entry name" value="GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090">
        <property type="entry name" value="NH 3 /GLUTAMINE-DEPENDENT NAD + SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006630">
        <property type="entry name" value="NADS_GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 8" id="KW-0520">NAD</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="9">
        <location>
            <begin position="4"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00052340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8138732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="685" mass="78607" checksum="B7010C8B8A4A0D43" modified="2022-10-12" version="1">MSITRIGVCTMNQFAMDFDYNLQNIIESIEICKRKQCLYRVGPELEVCGYMCEDHFLESDTVTHCWEAVSEILLHTDNIVCDIGMPVIHKSVFYNCRVILLNKKIHLIRPKIYLADDGNYRESRYFTPWSKEIEELELPTFIQKITGQKCVPIGVAILQTLDTEIGIEICEEMWIPIPTSASQALDGAEIILNSSGSHYQIGKIKERTELFKDITKRNGACYAFCNLRGCDGNRLYFDGCSCIVLNGKVLAKSDAFSLKDVEVTTCDIDLQEVRNIRINIKSRSLMASKQKHFPRIKLNINLTQQQNYIYYRDIPIQYESEVEDSIACYLWDYLRRSGASGFMLPLSGGVDSASTAISVFFMANKIFKTITTIDDDYNSHNKVLKQLRQIVQDDQFNPKSPQEIVNKIFFTVYLGTQNSSPESKYRSSLLAEQIGCQHYEVDIDEVCNACLSAIKPIVKEDPQFIVNGGTLQEDLALQNIQARSRMIVTYLLAQLTPWNNGKQGFLIVLGSSNLDESLRGFLTKYDCSSADINPIGSLSKNDLKELLDFCYNTFKFSAIKLILEAKPSPELRPLTAEGHVSEKDMELTFDELETFAKLRKVQKLGPVSLFKKLRYLWSDISPKQVAEKVKKFFKFYAINRHKVVSITASFHAQSYSCDDNRFDFRQFLYNWRWPWQFQKIDENSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SLJ0</accession>
    <name>A0A8S1SLJ0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0090012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SLJ0"/>
    <dbReference type="OrthoDB" id="10265645at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004019">
        <property type="term" value="F:adenylosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046040">
        <property type="term" value="P:IMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001114">
        <property type="entry name" value="Adenylosuccinate_synthetase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846:SF0">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00709">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8140316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="189" mass="22404" checksum="53E377C1D3261590" modified="2022-10-12" version="1">MIVIENEIELDLRGRDFLRCDTFLLKNHSVTTHQEIKREFNKILKKKSILLKITKIYYQSRNMIIDATSLISNARKDGRRILTKGANAIMLNIDYVTYPYVTQSSTNVDGVFKGLRILLFKLLKKRRKNFSTQLSNESGKMGHEFDKYWKTSSQMQLARYCYFKIFNLNQKTFINQSYQIRYLSFGQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SRG4</accession>
    <name>A0A8S1SRG4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0130019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="674604at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="219"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="261"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="303"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="345"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8144091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="388" mass="43514" checksum="6E493A242EF73EF7" modified="2022-10-12" version="1" fragment="single">MQSSFLQNGALTDIFDSVKDVDIQIFSVILEILRKEEGKDCIGFLSYIENQKQLELQILKQVGIITNADTEEILSHVREDMKKMTDVLKKLKDHDFNNKDFSSEENEVSTLSLMNSIKDKRTIVEFLQFLVQLTSIDETLIQCGSNAFHLLVQVKVDLRNKNYENIKIQNTSLVGANFVRCNLSGSQFNNVNINGIKLNGAYMFNCKWKNIKVHELNKLDGHRSCVNSVCFSPDGDTLASGSYDNTIRLWDVKIGQQKAKLDCDSYAVYSVCFSPDGNTLASCISDNSIRLWDVKTGQEKVKLDGHNSTVYSVCFSPDGNTLASSSDDESIRLWDVKTGQQKAKLDGHNNTVYSVCFSPDGNTLASGSNDKSIRLWXXXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SVJ6</accession>
    <name>A0A8S1SVJ6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0140347</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SVJ6"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8145641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="117" mass="14134" checksum="1672B9B5DBCB225A" modified="2022-10-12" version="1">MQVSLFLYHISYEQQRIDNQIYLILVLNHLNLLSFKNKGPINLDLYTNPILQPNNYQFQHEKEQLLFRMWNSMLILIQNITYSILQSKKEVGFKKEQFPILILSLIQIFMNSKIRQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T0R3</accession>
    <name>A0A8S1T0R3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0140088</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T0R3"/>
    <dbReference type="OrthoDB" id="295539at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="203"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="347"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="378"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8145129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="853" mass="97884" checksum="D34FE0B5CDF97ABE" modified="2022-10-12" version="1">MNGRNMPIRHHYEYSPQVQYRSLASLKIITPFCIVLALCSIVIGFVWYSFNGLTEYLLVGINNIAILFTCIFSLYWSFTSKYVVNEGIDQPEVYPKSSLPYLHSLFTQLVCAIEGGLVLSLIEYDSYNQFLLVLPIQILEIFLLLYMANVSRQFNIRDYGIQRLIVYGTSVVNLLFLIFASFSLKQVILQINSQLYQISPTNIEITLVKVVLFVLFITTLIISMFNLKRIKIYFVTLAFLILCICILACCINGLLVRRVQVLKVELSTKKGCKFAMQSISEKSLNDQMECPQKYFDLELSPYLPCEQDIQSYQWETNQKINKLGCINLKCCKAVKQYVFNQINSLTIWINLIVMVGIVQSFNAAMLSEGSYKKFRMNIVGDAMIFIILVLLAILVVVAYNITPELSVNVQHSLVKQETMLNQITVLPTPVYKNFDKQEKLVGFYNLQSCESISSIMIPKIVFKSTENQKGITLAISGTNGQFVALKEYNSENLKVIIDDEINKTIFQQEFQPNDGIIIKGDIDEVEKFYNNNLHYCSDNPLSADFDFMIDHYDLPKQHNRILETLTKEQQKIGDQAFKFFNVQITIENSADFSPIADALIEVYEGKFLSQSCQIIRQLENNLHALNTDEQGNAQINNLSQGFSYTVLIYKSGFKKSCSVLDLQRRVPKTKYIFRLTKSIPKHSVRILLEWTSKTLNLDLYSVFKVGEHSCLTGALSKSCGGMQLSTISKNDQHIEVLDIYQLEPQTYTIFVKRFLTRNEALDLLKNSMISQEWIDADPHITVYLSELNYPLIDFRLPQNINIQMDKLDLTWMVFKIDGQQSDAPQTIQKMESLITNEDIKSNTTSNKPFWPEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T2M8</accession>
    <name>A0A8S1T2M8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">START domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0160033</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8146750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T2M8"/>
    <dbReference type="OrthoDB" id="10252833at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00177">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000044">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051213">
        <property type="entry name" value="START_lipid_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308">
        <property type="entry name" value="PHOSPHATIDYLCHOLINE TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308:SF56">
        <property type="entry name" value="START DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00234">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="START" evidence="1">
        <location>
            <begin position="25"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8146750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="209" mass="24090" checksum="6AD37E18D99C351E" modified="2022-10-12" version="1">MQNNYDQLLEETKTKILSIVELKPENQWTLDTNKDGYIIYTRNNPENGLKINRTETNAAVDPEEFVNFVIDMTRKKEYDSNFLEGRLIEKLDQNTFIFYARGKPPTFFIDARDFCLLSRVYKLGDDHYMTITKSIEHPQVPVVKGVQRGEIVFSAWIVKKQPNGQTNIIIIGNMNPKGDIPKAIINQGAKFQAEGVKKAVEYLTKNKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T4P8</accession>
    <name>A0A8S1T4P8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0180049</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T4P8"/>
    <dbReference type="OrthoDB" id="283845at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8148405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="136" mass="16211" checksum="05D0D35665822BFA" modified="2022-10-12" version="1">MKQQYRSQTPVLGASLFDFNIPQMTITGQYKIRNACVLKKLCPEKKRNYNNPIKLVNPRKNMKYLFLNIDINDPKYYDSDSDYQYRSMPKQKVLKQNIQRKSMSIKHTNLEMSISNLELIQRPFCKHLRSKSLKFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T6R6</accession>
    <name>A0A8S1T6R6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0180331</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDC50/LEM3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T6R6"/>
    <dbReference type="OrthoDB" id="340608at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005045">
        <property type="entry name" value="CDC50/LEM3_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10926:SF0">
        <property type="entry name" value="CDC50, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10926">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03381">
        <property type="entry name" value="CDC50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="350"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8148965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="396" mass="45525" checksum="85EABA398DF3E814" modified="2022-10-12" version="1">MKQSKQSQNQAHDDQFSGSNTPNVHGLFLPQTMTMGQMSEDRKGEDQRLKLIRASTDPYEAFKDDDFLYPTKEDEMSLWQKYSGDYIQGMPVLPTGKCSLFCNIVCCFYFLIFGLVFVGIAGSITEIRLNYGKECEGKQQCIVNFEIEKNTYGPFFLYYELNEFYTSHSDFAQSISPKQMKGYELTDEEYDVYCPDTQSFESLQRPVGFNKSYAGFMIDLNKKVSPCGIAAKFIFNDSFLLFDVNTETATSLALNSTGIAFSVDLEYKYSRTQNSQFRQWLDLDDEKIINWFNIQSLPLVRKLYARYDNDLSKGTYSIVIQNNYPTSIFGGEKFLIVTTLSSFGSKNFSFGYLLIATAGVQFICAFVVYIKHRIVEKREKHEQKIHADLKLKEKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TAT3</accession>
    <name>A0A8S1TAT3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0190165</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TAT3"/>
    <dbReference type="OrthoDB" id="303704at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8149550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="139" mass="16647" checksum="79E81C1D41F2C7D3" modified="2022-10-12" version="1">MKIIEEIKNQVEQEMKKMIGKCIEKRYAKLTDKLKKEFIIKVIRDGQTIRQASKDLCINYSSAKAIISNYRKSFCSQGNQKYNLEPRQVQVNDKYDWKKFKIMTYCEETQTNEYTMETFAQNLKQSNQIKYQLTFENKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TD75</accession>
    <name>A0A8S1TD75_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DBF4-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0190301</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TD75"/>
    <dbReference type="OrthoDB" id="303526at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006572">
        <property type="entry name" value="Znf_DBF"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51265">
        <property type="entry name" value="ZF_DBF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DBF4-type" evidence="2">
        <location>
            <begin position="107"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8149793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="383" mass="46682" checksum="DB13375BC20E9158" modified="2022-10-12" version="1">MIHSLKPKKRCQQICYEHQFILIPKSSECWIKSQITLDYCPVYNEFTGQFENIIPILNCDRQPGLPTFILEYDRTQYQFNLERYQQEHQLKIEEQRIKKTKMLVPKPGKKHRYCGVCKKNYEEYLDHIQSQEHINNFNTYRSVNLIRTLIQGFQNVINHEFSDQKQSSILFCDSNTKIPMNKENFVAYFDQERIGIRFQKQQIQDIPMLEYKIPSSKRGRPNRNKQKKEIIKKAKVQEDIPIIPHYQPNTYLDYYYLQQQYLQAQQQIQNQQQQYLCYPQPQQQYFRDQMQYCNQNAQLDLQFYQSLMQQQQHFLQLKETEERREYSDRSSLWQKLIQLTNYQQPTQNEIVQLLHTIIEQYECNPRNNQNQNLNHNNNDQQFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TEF5</accession>
    <name>A0A8S1TEF5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0200170</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TEF5"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043169">
        <property type="term" value="F:cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000051">
        <property type="entry name" value="Protein phosphatase 2C-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="3">
        <location>
            <begin position="31"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8150258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="319" mass="36392" checksum="8146AB08CE767224" modified="2022-10-12" version="1">MLNIVDKLPSIQKQHMKRIQTEVQCEEQLIKVAAKTLPGQNNKKKLKINQDSLAIKQNLCNQNDCHLFGIFDGHGQNGHLVSQFISRQLPKTIENLLMQNYINNPNLLSQSLSLAFQQVEHDLVDKTNIACNFSGSTAVLILLIESRIYCANVGDSRAVYFYKFQDLWYNRPLSYDHKPNKTFELKRIIKLGGRVEQSLIDGKRQGPFRVWLTNEDVPGLAMSRSFGDVAAKSVGVIAEPEILRYKIQNSGFILLASDGLWDKMDFESIQNILDNFDPPLTQVDMELIAQKILGEAYSKWDRKDYGRDDITLILVHVQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TH24</accession>
    <name>A0A8S1TH24_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0230090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TH24"/>
    <dbReference type="OrthoDB" id="294472at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="17"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8152515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="283" mass="32807" checksum="844BC0A4B4D11386" modified="2022-10-12" version="1">MLQTIQLWKKVKTNLQSRFSNVKNTFDSIDTKNNGTIDIEELTFELQTNHGITSNNQIMQIFNLLNISKSQEITREEFEKLWMSDVTQLSKEEKELEKQPIKSYLKPQQSSILQKSGSSQQFSNLFDSYKSNNSPTKYINIQGDFTINQFNTPPLLDKTASPLKVISPPKQDQYPKSKLEVKDDKFRDMQRQISNLFNCKTEQPNTTPLLKQNFDGFLHTIKLGGSKKSNINISSKTKSPLLSNQISLQNYAFQFNDMKMQFNSKYNGEKINNNVKNFIYNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1THT8</accession>
    <name>A0A8S1THT8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0210247</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1THT8"/>
    <dbReference type="OrthoDB" id="285132at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8151244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="125" mass="14708" checksum="53D027A07348E594" modified="2022-10-12" version="1">MSTKVKISKRGQQYELSQKENYNPFSNLSLCPKSIIALAQSSYDPQLIEFTLNQLKTPCNSKHQTHLNRMRQIADKQENFVHGSISMNSQIETIVQYSDHSFYDQMETSHRKNSLHYFDESYQHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TIY4</accession>
    <name>A0A8S1TIY4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0220012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8151604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TIY4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8151604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="201" mass="24279" checksum="136A3AC371D6A67D" modified="2022-10-12" version="1">MNLIGIIRIIQIEKQELNYQFLIQMNRIQNTYKVIENFFQFHYLQISEKIFKALIRNMSQLKIQNRLNICKGFVNMEKKLKRLQLETDVYGQHMYIKIYFLNKLQTTDGKKQGIRKETIQNYWNKNQVYLLGEYVNDQRQGLWRYIFDGKQIGGGEYNIMYIEQHPYPLDQRIYKVLDNWQNSSLLLQVNTSYQSSAKNII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TN63</accession>
    <name>A0A8S1TN63_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0230062</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TN63"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17996">
        <property type="entry name" value="DEXHc_SMARCA2_SMARCA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000008">
        <property type="entry name" value="Chromatin structure-remodeling complex subunit snf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003020">
        <property type="entry name" value="SNF2-related domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029295">
        <property type="entry name" value="SnAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14619">
        <property type="entry name" value="SnAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01314">
        <property type="entry name" value="SnAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="372"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="679"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="996"/>
            <end position="1024"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8152459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1024" mass="122894" checksum="08083F1539323C11" modified="2022-10-12" version="1">MQQEADYNQDEKQVYQLRQQIICYKQVQRNHHIENEKLMFPYSKDQWETEREKIFQNSIQYFHDKIDKNEELKSIFRDRYPKLKLDQTCDDMSLFSERMQGYIEKRRQDIEQELKKTHQNDPKYVSLKIELLFINSKDFYLKVKESILNPLLQEENQAVLQSRMLERLLLDRNYFRRDKPQRRAESKLSDKFELSMIRHEQARRKKIKQKEFMSAIFAHQIEFMEFHRKKYKHARKRSVQFKVVLEQREQQRDKQMRMEHIRRGNLETYIQVLEKLDEAKKERVVSILRQTDQFLKDIGARVKIQKGEEITEEDEVIENMNSSNGLGYELSQANKVYYNITHKIKEVITQQPLLLEGGQLKQYQLQGLDWLVSLYNNNLNGILADEMGLGKTIQTISLLCYLIETKKNFGPYFIIVPLSTLSNWSNEFEKWAPSIKKITYKGSPQIRKEISKQMRTTKWNICLTTYEYVLKDKLTLSKYEWKYIIVDEGHRMKNSRSKFAMILGQQYQSERRLLLTGTPLQNNIAELWALLNFLLPKVFSSCEDFEKWFQTPLNIMGASEKDIQLDEEEQLLIINRLHQVLRPFLLRRVKKDVEKELPRKTEYVIKIKLSAWQKKIYDQINQRGVMTFDQQSGKSGSQALQNLMMQLRKICNHPYLFMLNLDMNKVTDEIWRSSGKFELLDRIIPKLLYFKHRLLIFSQMTQLMDIMEAFFEYRGWRYLRLDGSTKSEDRESRIQLFNQENSIYNIFLLSTRAGGLGLNLQSADTVVLFDSDWNPMMDLQAQDRAYRIGQKNEVRVLRLITATQIEGNILSKAEHKMGLDAVIIQAGLYNQRSTDQERRERLQDFFRQKNKVDLFEAEEIPDDTQINEWIARSEEEFEMFNELDRQRYEQEKLIYKNFNENKDDQYYNYRLIQEDEVPEWITSKQNEVQEVKEYGRGQRERKQVVYYLPEASPILEEQQDDMNELDFKMDDQIDIEQNQVYQDDLDLPKIQKKTKKLRQIENDDGNSNNIDENEPKLKSKRKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TNK9</accession>
    <name>A0A8S1TNK9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0230298</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TNK9"/>
    <dbReference type="OrthoDB" id="299606at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001020">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001178">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="599"/>
            <end position="831"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8152902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="852" mass="99097" checksum="45509BD26AA60F90" modified="2022-10-12" version="1">MSKNNTTISLTINQLKDQWQKSCKKEIVTQFVRQPSINQIRISKNNRSSNYRTSPNKLINEQNQTPKTLMQDNISIESTITISSQIFKSDSHIDNNQIQPTPQIKLIINQEHFHIDATTKLEIEDESNSQEQIQISNMQTELSQFRFINISNDIDNSINYLDLKFQPIVFQNSILSNTFQKKAESNKITHKMYMMQKLNKFKKDEMNKKIIKHDPLKESFLLNPELSLKIQTSQSIKLIKINPIQQENLQDQQLNKLDYEIQKSIISNDNYSFVSEVVQPKIQNFRKPLQQEQDNQSVQKLNLRIETIHEIKQYFIQTFPTISLKFQKIKMETVNIQQNSIHENNKLINDQPIKESILLKSQPMKLSFNLLKKDQIPLFQLFILIGEIQINDKKLQMNDQIPIKNYPTPNLNSNLMKSDIIDIDVIKVEISQNITKDCKINLISRSNQTTQIKQFKLSNLIKDDDNKPDQHLKCMNALEFNENLLKVNYEIIQPKEKPQYPTLLQMNRMKIEKIKELITEGKTSRATLKIKPTQKQDLYGIQSRQITDFNNSEKKCILHGSLIIEEAKETQVQQRCSSIDKATQNIKISKNQFSILNRFQKIKYLGRGNVSDAYSVFDQQTGMALALKTIQKSLIQSKGISNLISNEIKTQMILNHPNILKCYGIIDDKKQIALILELSDQTLYNFIRHNTITRKEMIDILHQVISAVNHLHNNGIIHRDIKPENILISQNVIKLADLGISIRTTSCSQYCGTLGYMAPEIKLHEKYTSKVDCYSIGVLIYEMLYKKLPLQTLYPIKNIKNDQLIDLMNNLIEPNQDLRYSCSQALDHEIFNQLIYPQLIQTSILNNLQKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TQR0</accession>
    <name>A0A8S1TQR0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0260300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8155075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TQR0"/>
    <dbReference type="OrthoDB" id="406368at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8155075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="307" mass="35532" checksum="630473921AACA1B3" modified="2022-10-12" version="1">MQRAQSAAPNIDFPMEDMFKIATRQTSGFGIEGYEAPKKYVDPIKQMEERKFLTQKKGQKNRNHVTKRGHYLEDLQKLYEKLPAPNKYDIVKPWVPLKQEGRVKSAPQKRCTFIDQIFKEAKIRGIPGAGKYNVIPTLEDVLKKVEEDKKKKIAPVERPTYLNEIQHLAVINPGPGNYNPHRIEKKLKINETKPSDQIAKHKEQERKRGKSCLPDIGTYNPEPVEFTSFNKLQQLAKKKDKSDKNNFGREDRFKDPKKSKSKQVPVPGPGQYPMIVLWAGKEKDKNNPKKKNYLDITTRGISRSIYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TU13</accession>
    <name>A0A8S1TU13_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0270153</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TU13"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8155533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="120" mass="14467" checksum="754DAE9BD06BB829" modified="2022-10-12" version="1">MIQRIVSNPSKMKETLLENQFKEQKVMIIHLNFIFLKLISSFACLRQRNCAIYQTYFQVEHYLFNGSSPSSICLRDFQHFQFLRRNDNSPDFQKTISRDKAYQRSIFSQIEIQKKQNMIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TUF5</accession>
    <name>A0A8S1TUF5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0270245</name>
        <name evidence="13" type="ORF">PPENT_87.1.T1480092</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000148">
        <property type="protein sequence ID" value="CAD8207731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TUF5"/>
    <dbReference type="OrthoDB" id="292600at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19502">
        <property type="entry name" value="RecA-like_PAN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000006">
        <property type="entry name" value="26S protease regulatory subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000044">
        <property type="entry name" value="26S protease regulatory subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000030">
        <property type="entry name" value="26S protease regulatory subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="169"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="15"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8155693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8207731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="393" mass="44147" checksum="49BF3D26794471C5" modified="2022-10-12" version="1">MSEKQSVKQYIQGKLDLLEFMIAEKQANVKRQEAQRNELNSLVRQVKEELQAMQEPHSYVGDVVKQMGKDKVLVKVNPEGKYVVTLDKSIKIEDCKPNTRVALKSDSYVLHKILPTKVDPLVSLMKVEKVPDSTYDMIGGLDQQVKEVKEVIELPIKHPEIFESLGIAQPKGVLLYGPPGTGKTLLARAIAHHTDCTFIRVSGSELVQKYIGEGARMVRELFVMARQHSPCLIFIDEVDSIGGARMEGERGGDSEVQRTMLELLNQLDGFESTQTIKIIMATNRIDILDSALLRPGRIDRKVEFPNPGVDARLEILKIHSKKMNLMRGIDLRKIAEVMPGASGAESKAVCTEAGMFALRERRIHVTQEDFEMSVAKVMKKDIEKNMAINKMLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TUU4</accession>
    <name>A0A8S1TUU4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0300246</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000030">
        <property type="protein sequence ID" value="CAD8157591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="305810at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="262"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8157591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1475" mass="177435" checksum="FF3FC984FE607D25" modified="2022-10-12" version="1">MIPANKSPQEVEIFFYYQQAINNLEQGRIDDGKVQLKNLLHHKIVQSQDNKTIHYATLISLAEVCGRSNQIHEKCEALKYYHESNAIIEDCWETNRKMAIIFRQIGLISRALHLTLKALESCQQAQGKLILYYQVCCLAFLINEVDYFNQYLERIPDENKLKQQLLELDQYIKEQKISQFVNELLEERNQISKKIAQIPKEYLILDYFYQEYECDIFLDEQDINKFFKKLKRLLINNLHIDDNSEDVHQKDISILLKTFFIIKSKKQKLVQINKLKQQHEKNVKQRLKQKLNLTNEQNYQEKFSLQSSLQKDNSQSIETMKKCGINFELISIFGKNTIINNEQIPDPEQNQNKTINNNQMGQYLIQKLSTQKFYFLTLLNQLTELIYKIPEELNIDQETIDQAQNQLIKCYIWAQYFMNELTNDPKIDLKILSQLFNEFKLRIKNEKNKTKRQIIKLKNYSKLIFKLNDKLLFQISFKKCQKEQQISQTKDFKMIMSQIHNEFNILKNFSIMNSNQNQLNKNQQLNKLLEFSLPEILEFWEEPNYLIEKGDQISSQVAKIKNFLKDENRILDPKETYLKILNACIKQLYFRKTKEQCKQVFQIIKPIFEQFYKIPQFNSIIALCFLKLSFIYGALDEEASYPLYLFLSFYYKKLDFPQKELLLITTLQLKQISLCKFLELFNLNQVIIDLLNLRQKMKQDYQKALRNFTETFQDQYDVQYYLLNQINEKPGIYGHCIVKKNQMQIETQPVETGKNVAPPESYSSESEDDYNYNFPYQINFKEIKLINEQLTDPFKIGQNQYQFSIEKQKYLLNHKFTVNLGDPKISGQIKRVSKEIIKQQYFISTKEIKKTFIVYFFENYYFSPNYIQKELQENEIKFIGYFLPICYNKFPDKKLVHNFANNVILKNIPKKIDTQVYQYANALNQQIFSKSPQQQPPKFNEMRDLIVSQKLQNEYMANIYYLLQLSREDEKNHIYICIAIALNEISFFWNYFYMKIFNLAYNQLNQKVQTQDVDWFICQKKIQVHIQKYQSLAHLFEYRFIMDLLKAQQRQLEYESQPNEKNYQKVQQAYQYLLQQNQITIPSDIYQLQHNFRAILLIEKKILRKLRSIPIERIIQSISNYSCINKLIIQMKEKQINYFYNSSEGEVESISDLQINVYKYLRRFIQSNNVSGLNKLLEIIGSKIPLLMENIKDNDEFQYQLSPELRFYKTQSQEKQNEALNQYLISKDFQQKINIVMMNLCSFFEKIKMRIDRAAPQRYVIEGIYYFCHIQLNNKNLTKEKLENLYQIISLIFSTQHKDLIKYQKRYGDEFCEQDQVLRYYYHHDLVFQYLKQKIIKLVMRIYILSKKWKELIELLELLPKQFDIRGYQICLALIENQKQFEDPQLLKDALCQIDYFLKRNQQYQILNENKEYNNMYVKLYDIFLIQEQNTKDTQSEIEKIFQAKKFIINQIKILKKCKRDTLQYQEPQNFIEIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TZF1</accession>
    <name>A0A8S1TZF1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0320312</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8159091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TZF1"/>
    <dbReference type="OrthoDB" id="5588846at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46809:SF2">
        <property type="entry name" value="GH21273P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46809">
        <property type="entry name" value="STROMAL CELL-DERIVED FACTOR 2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIR" evidence="1">
        <location>
            <begin position="208"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8159091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="375" mass="44072" checksum="BE0F6ED370D97D29" modified="2022-10-12" version="1">MQQFDQEILVSEVVQIRNTKTGSYLTATGFNTQEKGFLFSSTPNINNYYVVGSDDPNNHYTLWTIIKMFDDINRINYGDIVFLKNLSTKLFLIYEFEKFSEVSNQVRVSLHEKRQENYPLILQQQGEQIFQTKSYNIQSGVQLKIQTTKLTHFLQASNKNYTSKQVKEYKEISCSKDSDYNNWEIIKLNPEKQQLFEIKPTWQLKINNQEIFDSDKIIIRNYKSGYSLHSHKNKYSSTGMQEITCFSYERDVNDWWVIQQTFQMAQKQIQDQMPINLVHQETIKFLSVDEINLAKSKNGNQVRGMPSPIQQSFIISTIDNQQLIVGKPFFLFYQPINKYLCQGPSLSEMQQTQYEVIMTDKLSTSCLWVIEKKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U9C4</accession>
    <name>A0A8S1U9C4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0350096</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U9C4"/>
    <dbReference type="OrthoDB" id="296577at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8160657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="214" mass="24093" checksum="7FA66DB9D6C8664F" modified="2022-10-12" version="1">MLNSSIYSNENNSAIKTKGNQAHLLSTAISKLGDSSFKSRNQDSKNISPVRLRLFRDSPKTYKGLEGCVVGQYHPLAPTHVVFSNDKTPSPHRSARTFRDSPERAKVKLNQFQDWNSKLDIQLQLQNNQIAKLNNQLIQQSYQPYQHKSSKSNLFSASTPSNFQKFGRQSDISSLSSRLNQIPISQIGTFQLGHQSELNGLQSSLSRIMKTSRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UBS0</accession>
    <name>A0A8S1UBS0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0360261</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UBS0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8161587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="76" mass="9109" checksum="F7B2B1B5F8396C88" modified="2022-10-12" version="1">MIGDVLFHMLPQILGTYQQNNDHNNPQICLILNGILLFVFFDFFIIKLKNLRDKRDEPHDYDHNDSSIVFLFWRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UBY8</accession>
    <name>A0A8S1UBY8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Trichocyst matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0360052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UBY8"/>
    <dbReference type="OrthoDB" id="293977at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035886783" description="Trichocyst matrix protein" evidence="1">
        <location>
            <begin position="16"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8161202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="357" mass="41186" checksum="1CF21ED6D2EA7B49" modified="2022-10-12" version="1">MKIILLIFLIGATFARDEGFFAQLRNSDFGKTIIQTLQVQLSQEHPADTVVHLLKQMKDDLNNEQRVEDEEITGQLKTCQEASSAAVAVLTIAKERKATSEERLPLLQQEQNDKKQQLFDKQGEEQRNLDRISVLQEARNVQRQEFEQRRDELVGLVSALQEAKKILSQGIGALKKNSFVQLKNHHENFLKYFPNKKGFHSMVDVLLTVLQEENTQENAAQKVVKIIDTLVDSIFQVQKEEMKADDARELDFQTQKERLLLANRRLSGSVADLNAANERIGQKILEIKNDISIQDSIIFNKSTEQADWDQTCNDTEKAHRQQTEQRNAQLAIIVECIDIFETRFDMETKSYIQRLRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UD81</accession>
    <name>A0A8S1UD81_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">4Fe-4S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0370298</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I 23 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8162294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UD81"/>
    <dbReference type="OrthoDB" id="304186at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3270:FF:000001">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit I 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01351">
        <property type="entry name" value="NDH1_NuoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010226">
        <property type="entry name" value="NADH_quinone_OxRdtase_chainI"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01971">
        <property type="entry name" value="NuoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004538">
        <property type="entry name" value="PRK05888.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004539">
        <property type="entry name" value="PRK05888.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849:SF20">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 8, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE IRON-SULFUR PROTEIN 8, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="127"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="166"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8162294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="236" mass="28131" checksum="E278DA3F63039EE9" modified="2022-10-12" version="1">MLTKLLQKSRQARFCVYIKNSVNYHVANSQDPNSKFATPQFNSSILDFDKDQENNVQMHYYQGKFMPDELDWTDVWETAYQNFTQPEYLYHFWFCGVLYCFEPEWTINYPYEKGPLSPLFRGEHALRRYPTGEERCIACKLCQSACPARAITIETEPRPDNSRRTVRYDIDMTKCIYCGFCQEACPVDAIVEGPNYEYTTYQHEDLFYDKFKLLENGDKWEPQIARNIEYLITRKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UGR4</accession>
    <name>A0A8S1UGR4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0390274</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035841425" evidence="3">
        <location>
            <begin position="21"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="325"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="52"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="125"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="325"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8163544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="360" mass="40956" checksum="7812136FF02A6B16" modified="2022-10-12" version="1">MKSALLVVVLIACLQATTVSELKEKLSGYGDHPFGSSMINLVSVNMKTGGSLNELKQLLQQIKDELIALTQLQDQESATFTRRSQVDLAKLQATLEQAQQDLDNQRQEQSSLSNELSTLQTRVKEDQAALDRNSRGSGDAQSRLDAENTDFTAKYQDYSDAILACKEAQRLLMNLRGEGASLIQLTQDTKSNLIQTKENFQKIKEILEAHTKKSSLTLFQPIIEGLAEMTTKVNPETLNNVLSLVARLITALQEGQDQLEAXEEGKGSRIKDINLDVKDWEKLEEDKKEEQQQKMDMCYDICRLIYDEILPYSLEYYLDVHVEDDDYEGEDMDDDDEDDDEELTKEEAIKRMKERRGFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UJK2</accession>
    <name>A0A8S1UJK2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase S54 rhomboid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0420116</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UJK2"/>
    <dbReference type="OrthoDB" id="418595at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050925">
        <property type="entry name" value="Rhomboid_protease_S54"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43731:SF14">
        <property type="entry name" value="PRESENILIN-ASSOCIATED RHOMBOID-LIKE PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43731">
        <property type="entry name" value="RHOMBOID PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="5"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="61"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="83"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="107"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="4">
        <location>
            <begin position="38"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8165248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="160" mass="17642" checksum="2E4702BA06B892D4" modified="2022-10-12" version="1">MPLYFYLIGINVGVYALWHFPAVDKNFMYNHFTLHPGSMIFNMVTFYFFGRTIEAYFGSKRLLAIYMAGALVGGLMQSRQAGISLGASAACNALLTYYICNFPREIILLFFIPVPAWIVGLLILYQGWSGQGDGSGIGHDAHLGGCLAGLAFYFATRGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UJY6</accession>
    <name>A0A8S1UJY6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0420067</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UJY6"/>
    <dbReference type="OrthoDB" id="285302at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00454">
        <property type="entry name" value="TrHb1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8165150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="615" mass="73554" checksum="50B109D70C685E4C" modified="2022-10-12" version="1">MISDTQKSFKDFLETYAIRKKQPEKRRQPKLNPVFLLTKYQPQGNSIQIEQNFSYAITNPKVTNMDSQISLRFKTLESEKSNMSGLVLFRKNRWRQKEKNQCLIRNSELFNSVIQKSSRFHSQQQLAPLKSQDQIIKGYLMELSRKRKVAPKPQGTDELLNYEVDQIYLDITRRDNILSVKFPNKNYPGVKQIFRNLLATAYDQQMFLDLPRLRVIHLGMQLTHAHFFKFKYHFLTKYMHLNISTEKMFNCCERIENIRPYILNEILEFEIYGGEDGIKAITKQMYKKILSDVTLAPYFENIDVATQEMKFARLFFQLIYHLDSPNYSCEVLRERHVKYALTNVQLTNFKYYLSLTLQKTGIPWKNIRQLLRRMDVYKYAIINKNDLQYYVNQLGFNQFIENFVNSCAQDAMLSELIHRRGRQRFVAHCCNIFHYFFRYNIKAITREDLHLIHSKKAIINEKIFDKFKQKAVESVKDLTDDSLVLQDFIEDWDEIKPIILGETREQQILSLGQDYLIPKLVSILEYEFVQRHLNKIYETEESEMSQSILCKLNLLLYGVRFFKKQDLQIIHKRFKITSLQYYDFAQCFKIALEEYQVLNYVHQLIEEYEQFIVSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UKH7</accession>
    <name>A0A8S1UKH7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0410143</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UKH7"/>
    <dbReference type="OrthoDB" id="3596986at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8164657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="406" mass="46294" checksum="96709FFB5C4F5A4F" modified="2022-10-12" version="1">MSNLSRSSQSSRSSSQKRNTKRPQRDKRKKNNSDEYDSQIDDEDVSQYEGMRSKNKQQPLLGPGRFNRRENKLKKPNSVYTPAATTFLYKPGAELPNLSNFINQTIEIRIACEYVNKNNQALILRQIWGSNGVYASHSDAVCIGIHAGLLALGDLKLTGTFYQGISLSCKVIKGKKTLNGQLKVPLLSRSLKTPCAHCLKPEKVNWLSQLGSPEQLISWARKMSLPPNRRLTKKTHLNVFLHEPPQSSLPENLLVNNLPPLNEGWIVWDMCNEPSQKYNLLTFLDRQTAQGIPSRTSYRLKTNCLYIETSNKKFEISMDPNKIAEEVFLDSQVFMISEIIIPQQSDIEMLQKYGVPLPKELKSLILDKLKWINFEWGNDNVIIDGQITIQGLKSFKFVPITKHQHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ULH0</accession>
    <name>A0A8S1ULH0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0410099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ULH0"/>
    <dbReference type="OrthoDB" id="297868at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8164569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="263" mass="31043" checksum="9CE2953F76397D71" modified="2022-10-12" version="1">MSLRLELLKWVNEKCPSMKASNIESLSDGRHFLCLLRKYFPDIEVPQFKKSSSILQRMESLNLVAFYCQKLDASFKLDVLKIANKEATTILNFLKFLKSILDKTPIKNSFIQEEFQTISKVLEKDNHKICEEVLVSQVPQTIDDETQTHEIKEDLDSLHKFQFQLKRLMSLKLQHPYLELELLRLLDYDQEVADMMQHYQLAAQKRSKNSKHTDYHSIHSSQFYYRGEANLLDIDYSFDQQQASKRSTEFDNLFQVSDEKFII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ULH4</accession>
    <name>A0A8S1ULH4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0430128</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8165790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ULH4"/>
    <dbReference type="OrthoDB" id="310387at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8165790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="439" mass="53079" checksum="F86E46911A7C9842" modified="2022-10-12" version="1">MKGWYNQYIISQRVQGNDDKVRKIRLFENNEYLKTQEIKDHLEYVFPQSECFSLAQLIESMQCNFIKIYKGQVLIIILEILKHLQQLHQQKMSHGRLNPQNIVIKLKNDKDNKLTIQQKKFTIQRVQFINYRIIDDVKYEINQIQDNYDIINCCISLISTYQKQMCELFNFIITNLKLYQNRVIEISQLVDFFNLYIQECVDQQTQKIQKNQIYPSTIFQQNVQTVQCKEGEEYGMLSKRQRCSTILSKFLETFSGQLVNNKLKYQNNSNEYDIDEQQVEIIDIGNYNQEELQIFYVFEYILKQQMMKIIWKHFGNFFESTKQNIEQIYIEVIKIINQNNQRILNDHFKPILEEYQNSKKINDEINKEIDQNYKFNIQYDEQKAKDHFFLQLKIQAQNYDINNIQSFIDNIKSQVKQSLQEYYDEILEYEIISLNFDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1USV1</accession>
    <name>A0A8S1USV1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0420268</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1USV1"/>
    <dbReference type="OrthoDB" id="323142at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8165536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="153" mass="18277" checksum="DC3E66A3F170D3C7" modified="2022-10-12" version="1">MNIMVKQLDYPQFKIVKNQFLCQRLINLMKWKDLMDNCGILNKIFLKIVILFHLQQRIYLHFNHVVVNIYNSIQLIIPLDYMQNQGILSLRSWIRLLVVFSLKIYIHQITYFLKMNILQIQYNSILIIKIGIANLQKVLSLIKVEILETFLGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UZC4</accession>
    <name>A0A8S1UZC4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0530045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UZC4"/>
    <dbReference type="OrthoDB" id="295715at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070876">
        <property type="term" value="C:SOSS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044818">
        <property type="term" value="P:mitotic G2/M transition checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010212">
        <property type="term" value="P:response to ionizing radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051231">
        <property type="entry name" value="SOSS-B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13356">
        <property type="entry name" value="OB FOLD NUCLEIC ACID BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13356:SF0">
        <property type="entry name" value="SOSS COMPLEX SUBUNIT B HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8170410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="138" mass="16138" checksum="D813BA53E9DB1255" modified="2022-10-12" version="1">MMNQQVFEQLNSILFKQKFENQIKNMYPTQKNLEFKFIILKKIETQITKTYKKITKYWIADETGSAFLNLHDMDESAISPGDVCVMVGAYTNLFKGMMNIHQGKNGIFKKVSEFDLQYLTQPNHSLKNWGNDQQTTQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V1B1</accession>
    <name>A0A8S1V1B1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0530112</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10002605at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037869">
        <property type="entry name" value="Spp1/CFP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46174">
        <property type="entry name" value="CXXC-TYPE ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46174:SF1">
        <property type="entry name" value="CXXC-TYPE ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="8">
        <location>
            <begin position="249"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="973"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1184"/>
            <end position="1215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8170544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1647" mass="196922" checksum="E85EDDE4F5A75C92" modified="2022-10-12" version="1">MNCSVCGLNFLRKDKGCQLKECQKCKLKYHRYCYGYNNLDEYCDPCQDNVQKPECYICGQNGLLRRVSGYNEMYVHVICAIFDPYIQVRDYHTMSFSLQNQKDKKIKEKCSNCDQIGASIKCLDCQAVAHPHCIFKEKIQKELEQIENEQWILNLKFHGNNQDPNIIDVDQQEIKNELYDIQEAFSITIDNIFEVPQISEKSAKELKDEVLLNLESIFNNYTLPIKKQQFWSDYNKIESYCQSHMESHVIFCICRNSLDNQQMVQCEYCYEWFHFGCIKKKQIKQDYYLCEGCKKWNNRRQKIDLDNPILLNLEDLIIPKEIYKIYLLDVLPLLLYMEAIMRRLPRLLLTQDDYKNLKLIKSFLASMPIKPSAEIQLDKILLKQKLQEELKQKMLSLLPIQLEKNKSFFEELSKQLRNKGYSFDKEERKILKSFILSRKQMKFKIEKGINDGYLVETTFNLLEENIIDAHKLFIHPNETLFQLINRYILSSNIKKEFKQLFEGAKFQWDIPYIENQLELTVYKQYIIMKNQKSKPLVDKLIELKKMAIKSNITMGCIKLIEKLIQESTTLEDLNMKTNLQQIIEFPFNSEKLLNQIQSHFESQLIQEFKADLESKIEKNCRLTERDYQGLYSFDNIQKAAQESTIIKRVFDQLIEIQNRCKNQEKITSQSCSDILDLMDKCLFTNEYLENYKIKLRKFKEFDNKLKDILTIIELEQIDNECKSYGFELDTQSRRKELIQAIEIIQSKPNIIDCLDQYRYLKSGELELYIQQSQQQIEFIKQFYYIAYKRQDNLQIMLQKVKEIKDLDFSNEIISQVTIPQVVFDEISQIVLSFRQIQWRYECQKLLNESNNSEAQNGKKKYNINQVIKLLNSDIQIQDHYYLMLMQKIKCQYDIWLQTMKEFELQLQGSKAPEQYLLIQLINKNYYYEPNMQSELWSFYIQMLWMQKAEEFLESKANILSLKTLINCAQIANIKTNNQLLNKLKENIKIYENLNQKYKEYQEQRLIWLRNKELINNLQTYQQYFEFLENKPDAEIMSEIQNQAKQYQGLKLKELEDDLQEVKQVLFQYNELVQKQPDNNLYIQQLFKIRTCYCHCYLKIKGFSLQLMYNMIKQQSLRCLKQRSNNDQMQERLNEVDMLVTFGGEEWQQKLGLINKQRQKNTNSADLKFIDYSLRDDFEWIGWMKESMQNVNQILNQEKQNKKQLKEEKIQNKQQQSTKADDVWEDLRKISRERLKKILNKYKNWKQVNEQSILNLEVSIFQEMNLDREKYLKEMKKILEIIKLNKQKNLSYEEMRKIKDNTINKKTENQQSIIMEKHKKQQQQQQQQQQQQSIKIVKIASQSIPQIICQQIIQNNEQDKFNKPDIHFQQNKINNQTSISNPKGLNDKSKIKEKESLMNIGELNLLLKKKSDENRESWLIRPSLLTYDHQLIQYFPRPELITVTCETYSSAKAVVEYLTQKQSQFKMIIGWVYSKNITVAGDLFNLADKLKYSRQCVGTKQGNVGLTLIYYDFLKKLKPTTKWILMRDQFENQQIMNNLQNYHTLEKFKARLCFVYYIKDYKTSPNTILSQPVDYIDVLEEPKLRIIIENYKNLQYMQSTQNNQADSIQSQNIEQITKEENKDDNAQQNLNVHTMLKEIPGIFSLLNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V4J1</accession>
    <name>A0A8S1V4J1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">LITAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0530233</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDIP1/LITAF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V4J1"/>
    <dbReference type="OrthoDB" id="323534at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006629">
        <property type="entry name" value="LITAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037519">
        <property type="entry name" value="LITAF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292:SF6">
        <property type="entry name" value="FI16602P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292">
        <property type="entry name" value="LIPOPOLYSACCHARIDE-INDUCED TUMOR NECROSIS FACTOR-ALPHA FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10601">
        <property type="entry name" value="zf-LITAF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00714">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51837">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="LITAF" evidence="7">
        <location>
            <begin position="99"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8170762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="186" mass="21858" checksum="39F3E68C0253D968" modified="2022-10-12" version="1">MSYFQPSNKKSPNYIQPDIQQTNQIQQHHGPLYQQPQVYQQYQGGYPPQAGIQPIQYGGNPHNYVVGQPPQNVVYPQYQGYPPTFNNEAIYPLQISTQQERNFSFKNLFHGGIIANNNICIYCFRPYTLILQRKIGISTFIVFLLLLIFFWPLCWLPFVLKECKDRYYYCSSCGKLIYKRQCSFNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V4M3</accession>
    <name>A0A8S1V4M3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0550265</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="303010at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8171891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="280" mass="32538" checksum="25FAB41C8A6C4E9A" modified="2022-10-12" version="1" fragment="single">MFEEIKVSEILQSDIIEHSKITLSGSASNDIYQCTDSSISLEIDQTAYILKLANSQTVSINIYHMKEIYTKPISYTCEYVNFTCPITQNGCTLINDNNTPYLLVVTQVGHIYYRNLKTNTSMLLKNLMIENIKIQCFVKSLIQKEKFEFYLMTNTDLLYHVTLDVVGGEFQKNKYQISFGTKIFSKLLNSFSQDQKIAFQLNDKQILNFSQNYQFSLLEMQKTEFQTIQLQCQDRIKSNQIDIFQQKPSGKLFVCYQLNDNLKICTINQNQLIINYVVNX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V8J1</accession>
    <name>A0A8S1V8J1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0580054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000058">
        <property type="protein sequence ID" value="CAD8172803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V8J1"/>
    <dbReference type="OrthoDB" id="308412at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="26"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="262"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8172803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="480" mass="56693" checksum="76336A9400F37572" modified="2022-10-12" version="1">MLVSTNNTSKNYIIENTTRNEDQRTIERLRLLLKNAIQLNDELEKKNALLLYRIEQQQNQSQQSIQQEDYKHKIAKLQNQLSEVQSENHHLQQSLKNLKQLGNQNNQKNFELILQDCTVSQIEKQFYEFFERSSAILQEQMILEFQNILEKYQTKYQIVEIHKITSLTNLDKTKFIESQQKEIDILESKLNNTEKELCDLKRVLESFQQQQSMKQSQQDQVHDFFNSNQFEVFTSRLNNSQRIDECLSNDKRWIDIDNNNQIKTDERKLIQYEKIIDDQKQEIENLQNKVLNLSKKKNQLKQQNQELSAVLYQQAETCQRIISNLQAKVEKQLVQIQYYSSMQQMRSKLNQQPSQHSVQSSQQIDIRKKFTYHSPNTNNSPVSIAKLQTNNRSLKKQDSSNDQTTWMIKTTDASLVKKLDKKDSQSESTTDQVTKSTKLSTHLQEVKKSLDQMANGICQQKFLKGYYFVQNKPILTLDDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-09-13" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V8S6</accession>
    <name>A0A8S1V8S6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0580185</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000058">
        <property type="protein sequence ID" value="CAD8173041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8173041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="277" mass="32301" checksum="199B7875456EC3D5" modified="2022-10-12" version="1">MDSLPAHLRQQKKQISLILQSSQNLIQLSQKLVQTSQSEINFQPGPRLNPNQIESKSGDTGDLFEXQKKKLIQVNLVQKNKIRIAFKPIIRLRNKFLNGNNPNGYSLANYKEIVCNFCLLHLDNQYSKQEDNQSFSIITLEQHQQTHSQTITTQCKHKYHYDCFQNNIKQQLDQQKSTISCICGKKINNKLLKQYTSDVLIRKLLKIQLETIYSKYQESLNLKQCNTCTFFWVKSDTQLYLPICIYCLLSYNTNDTTKSSNLSQITKQKRLRSISMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VCW2</accession>
    <name>A0A8S1VCW2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RanBD1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0610098</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VCW2"/>
    <dbReference type="OrthoDB" id="2357150at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045255">
        <property type="entry name" value="RanBP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138:SF87">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE RANBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138">
        <property type="entry name" value="RAN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00638">
        <property type="entry name" value="Ran_BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00160">
        <property type="entry name" value="RanBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50196">
        <property type="entry name" value="RANBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RanBD1" evidence="1">
        <location>
            <begin position="17"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8174451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="153" mass="18255" checksum="A4F6C1DDEC500764" modified="2022-10-12" version="1">MSLDPAEPPIPEDPEEEIIPYVGPPKLLPVAIKTGEEDFELLMTFKCKLYRFRNEEWKQRGQGILKFLKHQETKKCRILMRQDATYFIRANHLVSETIVKSMFHQFGYEWIGQDHSDGETKLEQFSVKLESEEDFDKFYQQYEECRILNKDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VDC2</accession>
    <name>A0A8S1VDC2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0620010</name>
        <name evidence="2" type="ORF">PPENT_87.1.T0620011</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8174815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8174817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VDC2"/>
    <dbReference type="OrthoDB" id="306618at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8174815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8174817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="154" mass="18349" checksum="89AE76784199C275" modified="2022-10-12" version="1">MSRLFTLEDEQLDYEQDYESVLVVKEIELPKNEKENEKDLQKLLSLQFYSKKKKSSFQLNAIQRLITEQKENFKKQQENKYINQKLKTPSSKPFPVIIVTPKCQIKTPQYSKSSKQQILIAPLNFQDSFQKIKLLVSKQQKHLLDFSKFKRSIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VGJ7</accession>
    <name>A0A8S1VGJ7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0640024</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8175811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VGJ7"/>
    <dbReference type="OrthoDB" id="308638at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="71"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="124"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8175811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="189" mass="21659" checksum="332D50C4F2B54158" modified="2022-10-12" version="1">MRFSKFFGRILLAILYVNAGFSQFFNQDSYIHYVNIRYPVFYGYALNLIGSYLEYIEILKPNNFIQYTPNLILALGVTQILLGFGVGLGIQQAGYCLAFLTVIITGYAHNPLIYSNQVDINREYLQIIFNIGIIGALFLIRKNKNNKNVTVKNTAESDKKEQKNTEQPKQQNNEKSNQENTKKRKGKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VH29</accession>
    <name>A0A8S1VH29_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Anoctamin transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0650115</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000065">
        <property type="protein sequence ID" value="CAD8176504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="296386at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF73">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="686"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="800"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="833"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="883"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="921"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="997"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1039"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1073"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="8">
        <location>
            <begin position="676"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="468"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8176504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2900" mass="348033" checksum="C5F8CA8745C3D529" modified="2022-10-12" version="1">MDTISEDTPSQSYIKDASMQEKTDNPLNQEKQEACYLKRKVKAIQNICEKYQIFLQFLLGIDLNKHQIDILQQMWGNFSMETQETQLPDAIIKFPNPDYGKSNSHSISGIEGFELIKTLIKFNKGSIEEKILLKACEGVLQILDDSKEGSKNGRRKLFRQKVRRENQFIQFENTEESKRLETSATINEEDQKEKLKDQLILKLSNRLTGSWQFDSDQNQINEAFEIDYKLLKFLSNKKVNQGGYLIKYDNEWHRTKRDTKDFSTLIRNLICLILCRQGYYTQQFLSKNGKYIFCSLFATEESLKIQAESQGLFKLLNIQFIDLFSFEPLDKSYRPLRLNNRLWKPQNYKNLSQMFMYFRPILVELIKEIDFKRIAREVNQSGINQKLFNYGLDMYDDQDCPSDQEWAAYYFFLTHLHQQINATRQNFLINNDIAALINKQITPLELYLRRAQSENQINIDMHLYKTYYQDLEEIKNQKINKLKKQVNDVIEEYKNIFNLTTNPYTKLIKVLKKEQLAKKYLEIFKESLKVANNEKKYLKCLWDLVYLDHPLNMKTKYSNPQADRSLHHKHCQLMCWKKYQLNQGEKVTIFQSSERLRLAYLNIFQSFNISLLNQKGLIKQLFCLHDNFELYGIYYSSYENLSRNREQFYQEKLFDIATQLKFHLLNPMKMKFDLLCQYFGESVGTYFYFLCFFSQMLLYQNCLGLLYYLIQIPSNLQQYLSILEIIYLVCQLQITSLYTSKWEQQLKIFNQSYGVNSQEDIDTTSNKQIGEPKRSLINDQMNVPSINELDQIFRECISMLIFILIQLLYSIIFIALYFLCAWFQSLFNKTPTINNFEIIIIAILHLILNKLYTYHAQKAIQQLVDFEQQRTIQAVKQSYNLKLYSFSIIQQLGPLLILRFFNQSFQLVCESDSCEQYIGYYFLTYIIIKSVEKLCVFLFNFYSLRRQPLFIYKFQQHDINEYIEVQSKKPSNIKDQYFNCIEENLQQNMVELTILTYFAYIYPITYFMQWLLFVVQIYADKAQYLYYYQRPWPQIDCFVKVWNNILQYIILSSVIISSYGLSNSLVFENKSQIFVFVSFMLLSYTLNFVYQVWYSETPSYLQKLNLRQKYLVKSTLEKFKKKNKTINFVDNQNLKRMPIYKFYGLKLQEQVEQFETMSSDSDLEKYYIRNEEMEEPILQDEDIIIQKPKLKSRIRKSPIFVRSSSFYEKFMIQKKKKSKNEDNIQKVFHKVNLLLNYHKSWTDLRIIQRNYYIPRKKQFIKNFDILRYNIIQKSLQTQKSQAKQFIQKFYRKKIAKLKDSKEEHDIKELEGIYEKMRKYYNKHKWLNAQKFIFIKIKKAVWFNRFRKTSIRLNSKILIHVFQKRLSAKQYPGEETNFQHFEKNNKGFEFKEVSLQNIINFSQQYTQNNVKLIPLSAGNTNLKYYSASYKKSSYFKDVIEKVRDTYIFDTQKLILQSIMMEYFEEEMFTIPEINFVRQLNDTMWLANINEKKQPQLIQFLQLKHGQKLKYLQSINEDRGISYIQGECYTRLSNHIDKIDDFYVKGYCVIVYEQSPITTLENVIKYRKKYHLNYSYDEILSFFYDSLLLLLREQHGDISTKNYLLDNKSYYLGNAIKPDTDNDLFCLAKVLISMITLTSFKNSLNSLADMEKHDLYDIIIKMLNGSSNISELLSFLKYQEKFQESFVVERQENNENQQCDFSEYLQLTLHRGNFSLRMKQYEKGLIYIHQFEQILHRQIYKCGNNASEAFQGYVINNFMKYSICHNDMEEIIQITMVFYIKFCIQMQIKTRRYNHSIYNINNIQENTKHSNQLNQQFDIIYNCLGDLIKNLDLVLIQLQININLEQITKQEAQALFGITKNQSGNVIKLKHFERIQLINQLRLNKKLIENLFQFRKTIRRFQNQFNALNSLSHYFKMEMPIAAKKIDDCIEIQMELIKSKNQLELTKELKDLSPDLYNYNQMSPKENKIDAFTFQINFSMSQFCQDDPTYFHQLLYYYFLQIVIWHDSENDIYERKAKSFLLMGIENSPTYEYFCSYLKSITFDEIPIQYIQKEQECEIGKFECVKLQKWIEQTYSYQSEYILSTNFQYMWKQVLTYSNLNQNIHNKQILHEELKINPKDLKVQLYLIRIIQQILHLDCYFPLNYRISQLKYQVDQIDRDIYSNSYIAQLQRLHHSITIPFDSWFHSNGLLQFRLYHIQSIFFAFSPKHIENLENTENSLRELARNAVSVLNLQKDNRDILMKIQQILLEDTLLLIDVNLKKQQLFQILYSYLLLKIHQNQDLVQTIKIIQKEQAINIQPSIIILMILQILLQQQQYDQMEFLIQFCKKSISTKSILFAQMHENFENDLNIMALYIPNKKIDLNFIAPYDSLYQRSHSVIFYDDINIYSRHLIHHFFYFQNFFEVIEYFEDNLNQIENNRELTHLKQLYENFLIYIRVSIGIIKQEKAQDFILSIHSQIQDDNNTLIAAINKHILIRIYLNQKEYHVALQYSYQVLQFFYAYENSKQITLGGDFQNKHYKSLSKFQGILSNFLPKDLVRKTQINQQSNDVYYCHLIDDEFICEIILNHIDILTNIHQFKFSFFQVLNLLDKIKNYYQIAYLYKLLSLIFLENMNLEKQSLVQYDQQIMNEAKEIIRERKLLINCYQSFKHQHTQQEVIVYNQQLAKLTFKQIVYQKFAKKPAKEDLLFLIQLSAEKALEFFQILSQQQKITNHIFYINIYLILAECHLNQLNFTQALANVDLAEEHTIEIYGKACHPQIGYLLLSRVLFRKKQQELQIQIVKELKQNNFFDISQIKTITLLLIQENKTITYWIDIYKRSRDVQTFFKNVKDVMKLNQNFCDNFLFSDESCSVEDIFREITRLFPSLNYISAQSYLSASHHFIYLDENNQYRQQINQLLNNHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VHR8</accession>
    <name>A0A8S1VHR8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0640071</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8175905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VHR8"/>
    <dbReference type="OrthoDB" id="300630at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8175905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="266" mass="31853" checksum="367BD7CEFBC2443D" modified="2022-10-12" version="1">MDIRLKRFDVFGLSKNQQSQKRLDSKRKSEDKSFLQLKQYKHEFIRTPSNSNTCQTQMKKMRLKQQPQINQNQILSYSLSIQHIEQLPNRSLDPKVIKIILPNNNKTIQNLKEGLTTKRNYYDNYFAHNNQNEQKASFPTIKFKNKIVKSQFQANLQFYKKQQSIPIQVKLANQDDIKKELQTIRNRYVKSRTEFENSNDHNKPLLRTSKSKFHLLQQRNQNQKLINIINNSVESQTNIDPEYLEDFQKYDKMNLNRFRDELIQNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VKB0</accession>
    <name>A0A8S1VKB0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNase III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0690161</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VKB0"/>
    <dbReference type="OrthoDB" id="301959at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14622">
        <property type="entry name" value="Ribonucleas_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase III" evidence="1">
        <location>
            <begin position="76"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8178368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="314" mass="36981" checksum="316BDDF2F9CFA396" modified="2022-10-12" version="1">MFNQLLEFKNTKLLVQALTNKIHEMEYLQKTGQPYQYGNNEDFTIGGQQLLEFYFYDYMLLQGFPNQQSQFKKPSDIIQLRQKLINDKYLSEIAILLNIQNLLNVGNQKSLKNNPKVLSDCIKSIIGAHYYDKQNDLETLRDIIHPIVVQLLNKGEQITKTQWKYNPKSSFLEYLNSYKGELNINPKLTIEWKKDDSILNQNKSLYLISLELNNTMKIQKIGVNKRETEQNVYLEALLQLRKYHIKNYQKQNKLTNKKKEIQEKQCESDLSTSLDSTINNQDLSIYCFSEKTLDQSKISFDQQFNHILEQLANE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VLK2</accession>
    <name>A0A8S1VLK2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Kinetochore protein Nuf2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0650074</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000065">
        <property type="protein sequence ID" value="CAD8176422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VLK2"/>
    <dbReference type="OrthoDB" id="8194677at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031262">
        <property type="term" value="C:Ndc80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005549">
        <property type="entry name" value="Kinetochore_Nuf2_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03800">
        <property type="entry name" value="Nuf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinetochore protein Nuf2 N-terminal" evidence="2">
        <location>
            <begin position="55"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="167"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="301"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8176422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="417" mass="49850" checksum="E7170A995EFFCFFA" modified="2022-10-12" version="1">MNSNQELVQMLKDLQIYNSIDNNFNPDHSQLVMIYEKFCDLINFDDNFTLENLTLQLFYRCKLMCKTVGVVDFQMMDLTDPDQKRMKHILTLLCKYSIWKRDQTQKWEKRDKEIIQLEDIDLQNNRKQKSELEDLIQMKYKDMDLKHKKFEQYKQMKHKAESDLLAKRNLNLKLQNSISQNQNEQKDAEKKFKNLIEKLQVAQNELSILESMIVKDPKNLEKKVNDSLRRVEALQIEVDKCQKELQIETFKIGKIYKQLYQDYDSFLRVLEQIKESEKALIQEDKNFEKSNDQIFTASNKILQENLALQKLQQNKEQLQSQKLAESEKNNSSNQYSLQSLIDLQNKLQDLQAEVKKEQQDYLLIQQMKQEQQKITSENDQIKVLIEANLKQVVTQQNKEIEQYTKNIEILLNKIEFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VRM0</accession>
    <name>A0A8S1VRM0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Prolyl endopeptidase-like</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Prolylendopeptidase-like</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0690137</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Serine peptidase whose precise substrate specificity remains unclear. Does not cleave peptides after a arginine or lysine residue. Regulates trans-Golgi network morphology and sorting by regulating the membrane binding of the AP-1 complex. May play a role in the regulation of synaptic vesicle exocytosis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S9A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VRM0"/>
    <dbReference type="OrthoDB" id="248387at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023302">
        <property type="entry name" value="Pept_S9A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051543">
        <property type="entry name" value="Serine_Peptidase_S9A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11757:SF19">
        <property type="entry name" value="PROLYL ENDOPEPTIDASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11757">
        <property type="entry name" value="PROTEASE FAMILY S9A OLIGOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02897">
        <property type="entry name" value="Peptidase_S9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S9A N-terminal" evidence="6">
        <location>
            <begin position="16"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="5">
        <location>
            <begin position="515"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00039290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00042165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00045448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8178332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="759" mass="88365" checksum="0358110CBE17B086" modified="2022-10-12" version="1">MIIKRGLNFSRLIKEASKLIHGRQYIDHYWYLKSNPESLQPIIDNETKIKNTFFEQPQIKEIFDDYYQVSQGRKDGRTHNIPELYDEYEYNGRFAYFDSGNNYQIIERSLKEGEKEVVLDLKKIQFLKQYQATLKLTKLKISDDHKLIAFGIDLLNNEDVVWFMKYIDDERVLRTKLSDCFDAAFTKDNKHLIYTQYDDKFRPYKLMLHKLGTTQEEDELLFEEKDEQFYLDLQQTKDGKYFVLLSQTKQSNEVILIDREDPKKLQLLFSRKENAINLVHHHEEHGLYILTNKNNYDYKVLKQEGDKFVDFYCPQQGEVLQEVDLFHKHLVLYYTKESESFIKVISLQDKVIFNIIIPPSLVYTNDQKNTKNMLRNIYDRVIKKDKSQYFMNQKIQNEVLGATIEPGINHNYKSGVFQFHLSTPLVYDQVYQYNLSNKQLSKLQDFSLTGKTFKREEFTCTRYYAPSKDGTEIPITLVHHKGLQKNRQNKLLLHSYGAYGLPQEIPFNIVQLNALEKGWTLAYAHIRGGYERGQDWHRQAILENKIKSIEDLLGCAAYLIAEGYTHPSLLCGLGASAGATTLGAAINMRPDIWKCAILLSPFLDVLGSLLDENLPLTKSDYLEFGNPNDKKIFDTILSYSPYENLKKQVYPAIYISAGTGDFRAPLWNVLKYTEKLKQIAIKSKKVETIGDHPLIIDINDGGHSGGAGVQSFIEEQTRYMTFLDYYVGIGNKEITKKSLTQDIDESIQLADEEDKIQKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VSV1</accession>
    <name>A0A8S1VSV1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0700140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000070">
        <property type="protein sequence ID" value="CAD8178852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VSV1"/>
    <dbReference type="OrthoDB" id="302542at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="203"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8178852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="268" mass="32204" checksum="86313F0F03E3C133" modified="2022-10-12" version="1">MSRTNDFQTKVEKIIENYLQKHKIYNMKNSLNHVDPPISEFDMLEKQMTRNNMIDDLIRKIQVLVDITNDHIKIEIEQKFIRFEQEISNLRSIIMMQQDYNPPQLTMTRSENNSFIEMSDSFLNDENATSFKKKLKEIQEQFTKKTQQLDNMLTLVIDQQLQLKQQIIQSPSTIQSNEYQMDLNQQADKILDIIQPKIQFLNKEHFEEKYQQFKKVMEEMKKNAKEKQSLLMTIVDECKRDCEKIEKKFNEHITLVNAEKKKSWIPFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VUY2</accession>
    <name>A0A8S1VUY2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Calcium-transporting ATPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0760194</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000076">
        <property type="protein sequence ID" value="CAD8181544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VUY2"/>
    <dbReference type="OrthoDB" id="3352408at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF369">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="318"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="359"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="807"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="884"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="978"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1015"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1050"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="10">
        <location>
            <begin position="54"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="8">
        <location>
            <begin position="177"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase C-terminal" evidence="9">
        <location>
            <begin position="833"/>
            <end position="1067"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8181544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1112" mass="126535" checksum="65B2D95C71D38B6C" modified="2022-10-12" version="1">MQEVLSEEQNLSLSQDISLQGQKQRISKQTLVKIVSAAQERQFAEEIDELENIEGMEGIESGLQTNFEKGLKGDDFKERNLFYGTNQSIINSSKKTYVQMVQSNLEDYLIQILLVASILQIIIGVITADENCRSLAWIEGFSLFIAVFLCCNVTAIYDYQKEKQFQNLDERKDTLQTVIVLRDGEKIQLNQNQIMIGDIIYLFEGMQIPVDGFVIEAEQLKVDESSITGETESIKKDTYKNCKYKRDQLSDEKNCLTKFDIPSPVLLSGTRILSGECKMVAAVVGESSCIAKISSLFQTEDVQQSPLQLKLEIIAQSIGKLGLIFSVIIFFILLSRFIFQRIIEDSFEKEHIKEILNLSIVPITIIIISIPDGFPVALTLCIAFSVKRMLKDNILVRKLSTCEILGDINIVCSDKTGTLTQNKLRMVNIWNDNKMDIDVYSEKLNLNKYLPHQMHELFIQSSIVNGTAEIRPQERGSQTDVAMILFIEQCGFNYQNERDLHESTQNMPFSSRRKRMSRVIGDKRLVIKGSSEIILNCCNKIHSKSKGIIPIDSTIRKQIEDFSEQMNFQAIRTIGLAYKELNGNEDLVSKNEHEVYDIETENLILIAIVGFKDVLRAETPQAITDLQIAGINVKMTTGDNKITAKAIAKDCGILIDENQSLVLEGYDFNQRIGGIVCKWCRTSVCDCPRDQSNAKLLTKQIRVDTIENGEEFDKVYPKLDVLARSRPEDKYALVIGLQERGYVVAVTGDGTNDAPSLHKADVGIALGISATEIARESASIILLDDNFSSIVKIVLWGRNIYDSIKKFIQFQLTAIIVVVIITLSSSILIKQEVFKPIQMLWINIIIDSFASLSLATETPYQGLLKRQYYNRNESLICPKMFKHIIGQAIYQIIIILILIFYAHTFIPEFKGQEDESQDYFDKLQYKYSNTYFDVNNKLHICPNHQDYCNLISYNTEYNINGSENYLSFYKKTYIPSRQFTVIFNTFVMMQLFNFINARRIQDELNILQGIFSNILFPIIFLGILSLQIIMITFGGIVFHCYSFNGLCIEQWLICIAFGFGGLIIRLILSQIPDTILGFLVIYISKKNPNYQLQIPGHESETENLRNPNQENS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VV14</accession>
    <name>A0A8S1VV14_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thioredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0730146</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the thioredoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000073">
        <property type="protein sequence ID" value="CAD8180083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VV14"/>
    <dbReference type="OrthoDB" id="2121326at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005746">
        <property type="entry name" value="Thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43601">
        <property type="entry name" value="THIOREDOXIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43601:SF3">
        <property type="entry name" value="THIOREDOXIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000077">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="3">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8180083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="112" mass="12637" checksum="41823C8F50B4315B" modified="2022-10-12" version="1">MQGHILTIKTENDWKQLVLQNQKPVIVDFYADWCGPCKVLKPKLHNLHEQSQGKWELAVVDVDIEELQKISSQYASKGIPAVYLFHKGEKVSDFLGNNEAKAKEMASKAASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VW89</accession>
    <name>A0A8S1VW89_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Autophagy-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0750107</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATG8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000075">
        <property type="protein sequence ID" value="CAD8180917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VW89"/>
    <dbReference type="OrthoDB" id="366390at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004241">
        <property type="entry name" value="Atg8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10969">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEINS 1A/1B LIGHT CHAIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02991">
        <property type="entry name" value="ATG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0072">Autophagy</keyword>
    <keyword evidence="1" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="lipid moiety-binding region" description="Phosphatidylserine amidated glycine; alternate" evidence="1">
        <location>
            <position position="720"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR604241-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8180917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="722" mass="84813" checksum="5850EB12A09560C8" modified="2022-10-12" version="1">MKNKIKKQPPQYALKLTKLNQPLDSKNLKVLNLQGLQLQQFDIKVENLLTLILDFNNLRNLDIISQFPNLNTLSITNNQIEEFNVPSTLRILNISNNLLKTIHLKQLQQLDASSNQLQFIQQDQQNNLIQLKLDWLRIINQNDVWEHLTIKQFANQQQITFRQFLDKAIELQIQYGFKKEQNYFNQKIIHTSMIQNDKYYFDLILPYYKENRHFFDNSETPLCLAIKKLKMNFIADLMSNIPIKYEIDAFHESIKQGQISLVKQFLELGIDCNGFSQKGLTPLTNAVLNITQMNMEMIIHLLLQSQANPNKLNLNGQSLVQMCIIKSNLTALRFIINLNKKKQTKLKFKMNIKNTNGDYPLHLAVNSVSILYFLLNNQIGNPLQVNYQNLTAKQMPFSQNRPLIYKLLQKEERVQVAKQLAKNDMRFRILNKCQNTQKDYSSDSSCPELSEDEMPNKPKINRKYITEIDELSEDIRQLSEDEECQHNCSSESEIQYPKDNLKSLKLVQLNHQNYQLFKFDQHVRQKSILINKINKKRLNADQFNYYSRQGQNFKEKINSLICLRQELLLLGQIREYSDLICSDFASYMSKLNQTIFNLESDFQKDSLKDYMNFQQSLQVSIVDKYPDRVPILLEITDKSKIRFSDGSKFKKYLISKSDHFYHFFQILRNSLKLSKQEAIYLFVNNTGLIKPESQVGEIYSKHHSNDGFLRIILSEYATFGHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VWF3</accession>
    <name>A0A8S1VWF3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein SEY1 homolog</fullName>
            <ecNumber evidence="8">3.6.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0750143</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Probable GTP-binding protein that may be involved in cell development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family. RHD3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDO010000075">
        <property type="protein sequence ID" value="CAD8180987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VWF3"/>
    <dbReference type="OrthoDB" id="1597724at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016320">
        <property type="term" value="P:endoplasmic reticulum membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03109">
        <property type="entry name" value="Sey1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030386">
        <property type="entry name" value="G_GB1_RHD3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008803">
        <property type="entry name" value="RHD3/Sey1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046758">
        <property type="entry name" value="Sey1/RHD3-like_3HB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45923">
        <property type="entry name" value="PROTEIN SEY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45923:SF2">
        <property type="entry name" value="PROTEIN SEY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05879">
        <property type="entry name" value="RHD3_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20428">
        <property type="entry name" value="Sey1_3HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51715">
        <property type="entry name" value="G_GB1_RHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="8">
        <location>
            <begin position="1"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="8">
        <location>
            <begin position="693"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="697"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="8">
        <location>
            <begin position="717"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="domain" description="GB1/RHD3-type G" evidence="10">
        <location>
            <begin position="40"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="50"/>
            <end position="57"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8180987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="749" mass="88285" checksum="A38BEB71BD9F0285" modified="2022-10-12" version="1">MIKNYGDRYHLIDKKALEDRKLDKNQLEEFVKSSGIIDIGKNYNIVSIIGSQSTGKSTLLNYLFGTKFDVQNRQQSVGQTTVGIWVSKDVQNNVVVLDVEGSDSVERKSGENMVENQTALMALAMSHCFIINVFVNALGQHTSCQLSIIKIIMQQNLKLFQQDTVKHIIFVVRDWDEDSNYEEASRRLNGYLLNIWNEIPKPDHYKETDFHELFSVQVFTLAYYKMKKEFGEQTSELHSKLANQKDPNFIFKNFDYEKNVRWSDMPQYLSNIWEVISNNKDLNLPNEKILISNMRCQQIKLEALERVKQLNEDLSNRVKEQFVDNFGQQCQTILSLAKKIYDTDARDYHVEVYKEKEKELKDELINKFFTFFQRQTEQLKQYYMNILSENFEKLKRESIYNLPDKLNELDLLKMKFEESLTKSVIEVGLWQEEDHIKFFNQQFDNHLKSFVEAQLATFKQQLDNIIKSECDKIVSSQVLNISPKFWLQIEIDYYQMISDKYQKYDVLLTGLRVQQKQIDDYLNKFEEDSFHNLKQVISVASGRFKDQLFQQFKAQFVRAPDGQPRNWQKLTEEEIFHFYTEARDKVFSLLDLLRIRKIKYIKQSANFKQKAKTLLIPSGQKIQYQISSDTDSDDVVLNEVFYTQVKMQLAEDIDVQYQDAIQKHKQDFLQNIPKPFWFLLLFFMYDDVLRWMGNPLFLYPILIILCFVGFCIAIGLHSLPKLAFQWVFKTLNQAVVPLIFGGISKLKGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VX13</accession>
    <name>A0A8S1VX13_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0740093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000074">
        <property type="protein sequence ID" value="CAD8180382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VX13"/>
    <dbReference type="OrthoDB" id="293911at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="52"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="205"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8180382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="341" mass="41185" checksum="B3C67CC54BE2E670" modified="2022-10-12" version="1">MSRSMRKGNTPALNSQIRPFSPNLTFKTDSLENQVLQPCQEKAEVQYLRYEIQQERQRSSQLEMQLQCEINQRVQNEQLFTNDINILRQQNEDYKLRIQELEIKFRENSIYYQNLLKESNDKVVTMSMEIERLHNYQLNRCNDNEQILRQNDQQQLAQELIAIQKLVEDLRNELITKQKNIYYLQEQLQMTQQTQRDNIGSTSKLREQEKNIDQLVSQINVLTQILEDKDNSLQQQSKQVENIYNELQLITQFNRQLNEENRNQKQALEQAYKEIERLNRIIMIKNQELQDSYHNENLDWRRNFQDLNEKYHKAQEQLCFAQAELEACRAAQRMTYLQQEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WAQ4</accession>
    <name>A0A8S1WAQ4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0850113</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WAQ4"/>
    <dbReference type="OrthoDB" id="298812at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="206"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="57"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8185495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="231" mass="26740" checksum="12B97A3CE50A8DE7" modified="2022-10-12" version="1">MQQVNVPSQYPNIPFTNQTPINKTNTSHSPNQRMLFQSSSPIQDSNSYTPVKQLGELELLYQKIHLLQNENKMLKTQLENANLQIKQLSTKINTYNSQAIFKTTTFELVVLQKALEQLEQLKTVLQKRKTSPLKNNQTLPTEETAEVISELKSKVTFLEQKNNKLCKENSELQQLILFPAQSETKEFIREGRKQLFSDPSEIRQVNRNSPIRFVNRKGSPSNPYRSPKHKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WCP1</accession>
    <name>A0A8S1WCP1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0900184</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8188058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WCP1"/>
    <dbReference type="OrthoDB" id="296534at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="111"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="3"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="71"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8188058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="27162" checksum="89AC652B2728D384" modified="2022-10-12" version="1">MDSNEQDKEIQFLQKRVKLTQQENELLRKENQLQRQSLTQMKNTFTNIISTKRIKTADGRNNSVSERFQNEDALRQVLEMQRNRINQLEKDNEQYKLKYEKKFTNNLTKQIFFYQYELIVISCNLIFFTVKYFLEAVIQTYYQLRRTNQIKNFLIYIKYIQTCSSFIIEIKNSLKIENPSIYSHGIIISRYIKKNTLFLDYSNSKQKGLQNYYKHTQPNNTYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WEP6</accession>
    <name>A0A8S1WEP6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0830046</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000083">
        <property type="protein sequence ID" value="CAD8184406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WEP6"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8184406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="177" mass="21152" checksum="9048240FB3DB060B" modified="2022-10-12" version="1">MSFRKTTFSQYSTNVLKLDNDQDYSSAKTLQKSNQTITTNYNSPQGNLDSLDNLLDQQSSKKSRFFFSQKIDSEESTRYIKTESDVQQKEVREVRPFRFRRKEVQPKIDLNDKNDVDDFFTGVEIPRISQQTQKQNPYQLVTFTDYVENLYGKDWFQQRERKFKRQITVQDFFDTPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WEW2</accession>
    <name>A0A8S1WEW2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Aurora kinase</fullName>
            <ecNumber evidence="15">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PPENT_87.1.T0880198</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="15"/>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8187202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WEW2"/>
    <dbReference type="OrthoDB" id="377346at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14007">
        <property type="entry name" value="STKc_Aurora"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000235">
        <property type="entry name" value="Serine/threonine-protein kinase ark1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 12 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="17">
        <location>
            <begin position="6"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="87"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="165"/>
            <end position="167"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="214"/>
            <end position="215"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="13">
        <location>
            <position position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAD8187202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="455" mass="53595" checksum="8A8584DE360869C6" modified="2022-10-12" version="1">MNVCSSRYCQYNLAGSQMVPIPKYRVYCPFCKHSYYCSQRCRDIDWTSGHKNQCIPQNMLNSQEIAYSDKVSTLRQMKRSPEEFELIYDYPQLGKGSFGAVRLVKDRTNGQLHAMKIMNKKDIFEYCSIENLKREIRIQRKLNHPNITQLFHYFEDKDKVYLILEYAEHGSLFQLLRRRGKLNENEALKFFKQTCLGIDYLHQQNIIHRDLKPENILLDVADNVKICDFGWSAENLGSKKRSTFCGTIDYMAPEMIEDRPHDHTLDIWCLGILLYELLHGEAPFKGKNDIEKCNNIVKINYQIIDNNLSSDVKDLITGLIKYKQKDRLTIKQILNHKWLKKNEFELEMLSIPQNRVRVNGSTMHQENQYLSKSTATSQQSFLRQNLTFQTQTENSIQDYKQQYRRSRHSDSQKKEIRSQTVKQKNETFMQKLLVALGCVKRDSYVDPSCLDTQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WFK3</accession>
    <name>A0A8S1WFK3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aldehyde dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0890085</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 6">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000089">
        <property type="protein sequence ID" value="CAD8187455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WFK3"/>
    <dbReference type="OrthoDB" id="440325at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006081">
        <property type="term" value="P:aldehyde metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07087">
        <property type="entry name" value="ALDH_F3-13-14_CALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000003">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000004">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012394">
        <property type="entry name" value="Aldehyde_DH_NAD(P)"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570:SF16">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE TYPE III, ISOFORM Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036492">
        <property type="entry name" value="ALDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="8">
        <location>
            <begin position="11"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR036492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8187455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="519" mass="58228" checksum="074121C82559DFF0" modified="2022-10-12" version="1">MAQIQDNSEIIQPIFQKLKETFNTHKTKCIKFRKAQLRNLLKGLDEMEQEFHIALEKDLGVTIFSSQMTSTIVTKVEVENQLAHIDEWSKEENVDTPFVIGPGSSKIIYEPLGVVLVIAAWNYPLYTGIPPMAAAIAAGNCVILKPSEIAPHSSQVMYNLVTKYLDQSCYAVIQGGVEVSKKITTLPFDLIIFTGSPEKGKLVARAASENLVPCILELGGKSPTIVDKDCSLNVTVQRIIQGRFINAGQTCVACDYVFVHQSIKDAFINEMKTELKRFFGENPQKSNDYSKIVTEFHTQRLKELLKDHNGQVVVGGQVNVDQRYVEPTIILQPQVQSKLMTEEIFGPILPVLTFDNINEVIQFINSRPKPLALYYYGSSSKNKKLLEQQTSSGAIVHNDSLFHLLNPNLPFGGVGNSGYGAYHGITGFKSCCHAKPVFTKSTLNIYPFNIRYPPFTKNKLQTLSLFFKFAELPQRHVVNATLFVSFLVALKLAQKKGYLKRLLEISKPYVEKVWKETNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WGN1</accession>
    <name>A0A8S1WGN1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0900233</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8188151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WGN1"/>
    <dbReference type="OrthoDB" id="308313at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="35"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="26"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="36"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="70"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="104"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="138"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="206"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="240"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="274"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="308"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="342"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="410"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="478"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="512"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="546"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="580"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="614"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="682"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="750"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="784"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="818"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="852"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="886"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="1015"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="1049"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="1083"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="1118"/>
            <end position="1151"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="1152"/>
            <end position="1185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8188151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1247" mass="147666" checksum="51B03D4D96D204DF" modified="2022-10-12" version="1">MVQFFIIAANIVDKLNRFIEAIKQYDSAIQKNPENSDLYYGKAITLEKMNRYEEALENYDLAIQKNPQDAKYYSGKAITLEKMNRYEEALEYQDQAIQKNPENADYFQGTAIVLFKLNRFKEALEYYDLAIQKNPENADFYYGKAITLEKMNRFQEALENYDLAIQKNPQDAKYYSGKAITLEKMNRFQEALRYQDLAIQKDPENADQFKCKAIALEKNNRYEEALEYYDLAIEKNPENSDLYYGKAITLEKMNRYEEALENYDIAIQKNPQDAKYYSGKAITLEKMNRYEEALEYQDQAIQKNPENADYFQGTAIVLFKLNRFKEALEYYDLAIQKNPENADFYYGKAITLEKMNRFQEALENYDLANQKNPENSDHYNGKAIALFMMNRFQEALEYYDIAIKKNSENADYYIGKAIALEKMNRFQEALENYDIAIQKNPQDAKYYSGKAITLEKMNRFQEALRYQDLAIQKDPENADQFKCKAIALEKNNRYEEALEYYDLAIEKNPENSDLYYGKAITLEKMNRYEEALENYDIAIQKNPQDAKYYSGKAITLEKMNRYEEALEYQDQAIQKNPENADYFQGTAIVLFKLNRFKEALEYYDLAIQKNPENADFYYGKAITLEKMNRFQEALENYDLANQKNPENSDHYNGKAIALFMMNRFQEALEYYDIAIKKNSENADYYIGKAIALEKMNRFQEALENYDIAIQKNPQDAKYYSGKAITLEKMNRFQEALRYQDLAIQKDPENADQFKCKAIALEKNNRYEEALEYYDLAIEKNPENSDLYYGKAITLEKINRYEEALENYDIAIQKNPQDAKYYSGKAITLEKMNRYEEALEYQDQAIQKNPENADYFQGTAIVLFKLNRFKEALEYYDLAIQKNPEEADFYYGKAIALFKLNRFEEALEYYDLAIQKNPEEADFYYVKAAFLKNIHRFEEALKYYDLAIEKNSEKSDYYYGKADNLELMYRIKESLFYYDQTIQKNPENANFYYCKAIALFKLNRFKEALEKTQKKADYYKCKAIALFKLNRFEEALECYDLAILKNPEDADQYNEKAIALEKMNRFEESLMYYDSAIQKNPENADYYNCKAIALEKRNRFEEAQQYYDQAIQKNPEIAEFFYKNKAIVLFKLNRFEEALECYDLAIQKNPENSDLYYGKAITLEKMNRFEEALGYYDSAIQKNPENADYYKCKAIALEQINRFDEALEYQDLAMQKNPLKKKQIIITKLHLKQHFLNQLQVSNYESNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WH64</accession>
    <name>A0A8S1WH64_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0900134</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8187967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WH64"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8187967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="76" mass="9055" checksum="BFFD141C2AE3BD77" modified="2022-10-12" version="1">MQFQIIMTLHFSKIKQNKQIQIIISVLCLIHHLYIQFDQFLIQVNTFLMKIITYSKLVFINALHIQLKQLLKASLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WNR0</accession>
    <name>A0A8S1WNR0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0960032</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000096">
        <property type="protein sequence ID" value="CAD8190271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WNR0"/>
    <dbReference type="OrthoDB" id="315997at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8190271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="767" mass="90430" checksum="EB5245C03CD4AE1D" modified="2022-10-12" version="1">MLIPKILEKEIDFQCQSQDLQPPEFVIIDSSLPHNKRFFCKSCGNQRQSTFKIIDFQSLRKSFDELQREFIQQNESIIRLHIESVIKLENILINLKTRQIHQFDNLLLLLKEWITALIDSVSSYSFFKQLDSLFTLQLFDNDQSNLIKNITKIHSYFQAQFDLDLFREQPEFISVQEIFQFLNNLIATQNVELQYNFRVERPKNQFLSQLIQSIRNMQEKQQHYIIEDTICPFGIFYRYKKQFYITKNNKEVKYQSLDGEIVRIDRKNKNQHIEILKKWEQIVYLQWKGQYDNNKQQVGYWTATWKGEIIKDVGGIYNQFGKKEGLWKDLIRNYWSIAQVYEVGQYQMGLKQGNWKFIYKDQEIGCGEYSKQGEKTGKWLELSDMFCDHSKVTQMGEYNQGKKVGRWDILFNYQGNNSKIGGGEYDQAGQGLKIGQWIDISDGFFEDSQVTYNGQYKNGKKVGKWDILFNWNGKNKIGGGFYDLEGEGLKTGCWVDIYDQYQQHRSITYIGEYKNGKKVGKWDIWYNYLGKNNNIGGGLYDEKGLELKIGQWLELSDGFCDYSQVTYQGQYENGQKVGKWDIWFNEEGEKYKIGCGFYDKGGQGLKTGQWIDIHDQYLQSRHITYYGEYRNGKKFGRWNIMYDKNEIGGGQYDEAGQGLKIGKWIDISGGFYEDSQVTYNGEYKNGQKVGKWDIWYKKDRKNIMIGGGSYDEEDQGIKIGEWIEISEGFDCYYEVTYKGSYQNGIKVGTWIEIFQGFNRIKEIQYDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WQB3</accession>
    <name>A0A8S1WQB3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0960078</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000096">
        <property type="protein sequence ID" value="CAD8190359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WQB3"/>
    <dbReference type="OrthoDB" id="300553at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8190359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="149" mass="18212" checksum="CB3FA9552B7F9A43" modified="2022-10-12" version="1">MLQQMRFNFQQQIRKSKIEDQLKEARKKYYNYGDNFNEQIILNQNSSSEFQQKIQFELKNLKKQIEDDENYIISLAKSKFLEEMATPTLIQNYSTINSIFFEIFIKMIEYAKRNNLFWIILQFRHSQFLNCLQSLEYSNEFQRFINSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WWF2</accession>
    <name>A0A8S1WWF2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1020093</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000102">
        <property type="protein sequence ID" value="CAD8192665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WWF2"/>
    <dbReference type="OrthoDB" id="312917at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd19757">
        <property type="entry name" value="Bbox1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="396"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="346"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8192665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="482" mass="58030" checksum="4CDDAE9A18A3E247" modified="2022-10-12" version="1">MKCQQCDRVPITYWCLDNICEKRFVCDQCSKYHGHKEKISQQIQKNKIKTWIKTLQITFLNIIHRTTCYQEFIEENFNQQENLEVLKKQHKLYFQFFQSKPKIQRAILNLNNQLNDIPISNIHKQDDPKYINKINVCKKLLQEKQLFKAQQQLFQLNPGESVYFQIECYILQKKLDDALKLSKETLGRIQENKYLDFNLQIQQAKILHKLKKYKEAIEVLKKVKEEELDQIQKQFYYLQQAKTYQKIIQYQGLGFNTIMNINNQYNLKNGSQIQISFLDRIQKQLEEEFKDFSTQNEDKKKEILEKNNKQKYFIDNFQFSLAIAKANLLRINNDELFLYDNNQNQQQNDQNQEQNNSNQQPNSSNQQQIVQNQQKFTLQDIELIYKEVLKEDVLNQKALFGLSQIYMKQIKYVEAREILYQLCQLNPKNSKAEIGLIYIKQRLLEQKIQDELDSNFSYTENCLSFIFRNFQRFSNQIFPEQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WX55</accession>
    <name>A0A8S1WX55_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1030154</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000103">
        <property type="protein sequence ID" value="CAD8193171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WX55"/>
    <dbReference type="OrthoDB" id="305626at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="95"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="609"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="374"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="439"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="617"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8193171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="647" mass="76136" checksum="B2E535210EAD4080" modified="2022-10-12" version="1">MDGGGSTFDLTAPAQFLQPKINVSKQPEQPINKPQKDLQQKTQNSNSKLPQLNNNQNAIKNANQKQTQQVQQQSPKAIQKAKKIDLFRDALQQATTKYLPPGKKEKDKTQKPKPQQELQQQQIEEIKKEYEDLVQNHYKDLYVHVANEQQKSLQDLHNKRIVTALQDLESRPKIQNALEKKEELINKQRLYKDRLLYDIKQMIIKSRRLDELLNDLVLQNEDTVKETIEELLEKEYIQEEKMAKQSYEIAKLRDMHLKRRKLLLEVQKQCQITYDGLKIKYQQYEASSQSMMSRKKQLESLSKIVAKYRSEFQTQQNFFGASLRGRFDPMLEEILEDELSKCGDEQALEIVEQGEKKIIAIKEDNEKRKRGEQKDKAKLAIENATQNINKQKQDIHQELEKKKQMFQKLRVVTSISNQEIMKKYKLNMDLDQEELKNIQEYVGKEIERYERQNDELRAEIKKLKYEQENSILDTQINLDELERDVTRQQDLFSEKEKQVKKVEKSIDEVTMSLSRIMYQLSGKGMKAKNIEIKRSALVATASTIQLRLERMLTVLSKTQEFLNEESINTNPRYNKVEDFICLNPKSYISQDINEQVEGNIKFVYKEEDSSDEDCKEMDEVRQQVKSKAQEDKPQVVVAQKKDKKPNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WZ33</accession>
    <name>A0A8S1WZ33_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1050024</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000105">
        <property type="protein sequence ID" value="CAD8193750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WZ33"/>
    <dbReference type="OrthoDB" id="292704at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="234"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="287"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="331"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="364"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="3">
        <location>
            <begin position="477"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="772"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="817"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="772"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="783"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="817"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="840"/>
            <end position="858"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8193750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="942" mass="111679" checksum="AE6E12F0FEEE9B9E" modified="2022-10-12" version="1">MNSELGSITERAPLKTQPTLPKQQLRPAFLKTVNLKTIHTNRMNSFQNRIWRDRALLVLIQVLRFISLITRSPFASKFSLIDRNMFRIIGDKAADFNYYLLHDYFKYMKPEQSSRVKYFLQQKLYKICYMKGFMDYVTNLRLIFEPESILLLLWNILQLININLNILYITVKFSFDFENYPPESYEFCEIYLFKVPYFLYLIDIIVQLNTCYYEAGYLVRDRNKIWYNFYKNNFFNNLFILVPTSIYFLAFQDSPLYLFMLLKCLSIPAITEAIIDRVELTTNYWIIYDLIRLIYVILYESHICCCGLFYVGLLNKESSWLLQNDLINETWIIKYLSSFYWSIITMTTIGYGDITPQNLFEKSFLIFIAIFSCCTFGYSINCIGQIIGQLQSKNHQIRVDLNDLKQYLRVRGYNNKLQIKILRFFEYLWKDQRNENQLDLNKFNQQLPSHLHSVRQIILQEMMIDLNMKSISKIPFFKENFNEDFISALASKFIEEKLVPFNTIFSKNDPSNFLYILCDGEIEYFVEIPEGSATILSIQTITGQDEIFGQQEFLLDQNYEISCRSTKSSRILKISKTDFNIIAKKFGYEKYCQLKDLVKFSGRFDEFHLHCVGCNKSTHMLYQCPMLTGFPNKTKIIIQYRKNQLQERKFHTRNNLKRRLSSLIFEAQISDTVLYYLLKDPKLSLQISSQYYIQTLKQQQIQKEFSNNNLNGHKQIQDEIRQTTRKPSILNIKGRKQSQYIKRLPDFLNGQYINFKSMPEDKIKKGVENVSKNVSAQHSETPSPERKQNTKMKTQEEINIFEQHRISADIAKTLSFKSMNQTSQDQDQLNSDTDSVKENSQLSQQNSSMSQTSSESPSKNSKDVSPGIDQITQFYMGTYKDIFEKFDKFQDFQNYMTHMNSSRILEQLNQFKETQNFTDFMNVNFVRKRRSKRSKLVIKIDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X300</accession>
    <name>A0A8S1X300_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1080021</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000108">
        <property type="protein sequence ID" value="CAD8194899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X300"/>
    <dbReference type="OrthoDB" id="19588at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050498">
        <property type="entry name" value="Ycf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE SPINDLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="32"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="66"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="100"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="168"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="202"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="236"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="270"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="304"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="338"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="372"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="406"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="440"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="474"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="508"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8194899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="592" mass="68932" checksum="15481119F48C02FC" modified="2022-10-12" version="1">MYQKANSLNELNKLQDAQQIYKDIITINPKHELSLLKIGDILRNQKQYQQAQYYYQKCISINLNNQHAQFGIAESLRQTYQYNNALQYYQKAIEIDQSNKIYQFYQGDCLTSLERYQEAINVYNKVLIKDPNHIEAMAGKGICLLYLGQNDEALKIITKSLNLKNNCLLGLYGYGIYLGSNNNYEEAIKYFDKALAINQQHVLSLSGKGECLRLMNQIEQALEWYEQALAINPQHVLSLYGKGECLRLMNQFEQALEWYEQALAINPQHVYSLSGKGECLRLMNQFEQALEWYEQALAINPQHVYSLSGKGECLRLMNQFEQALEWYEQALAINPQHVYSLSGKGECLRLMNQFEQALEWYEQALAINPQHVYSLSGKGECLRLMNQFEQALEWYEQALAINPQHVYSLSGKGECLRLMNQFEQALEWYEQALAINPQHVYSLSGKGECLRLMNQFEQALEWYEQALAINPQHVYSLSGKGECLRLMNQFEQALEWYEQALAINPQHVYSLSGKGECLRLMNQFEQALEWYEQALAINPQHVFSLWGKAFILLEYKQFENAYQLFNQANEIQPNQQLIDAFLAQYSNKQEQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X500</accession>
    <name>A0A8S1X500_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1140099</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000114">
        <property type="protein sequence ID" value="CAD8197073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X500"/>
    <dbReference type="OrthoDB" id="421226at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="307"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="515"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8197073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="954" mass="112631" checksum="10B7B1ADF265CA58" modified="2022-10-12" version="1">MSFHYTNYQSIQVNKDRIDTQRELDHQFTRVAARKNSMAIKTKSIGQRILNFVGKQMSVDYQERLVLKSLQAQNVNAKKFIEILLKQKHNLKNLKHKHLQIINDKALGNIDKISTQSSFKIFIDKLLKKRLLRIFKPIVECFSHCIIAIPLLYPENRRIILWDIIAILSKLYFLYLIPLELAWTNQSLMFNRYYTSTIVMLIILFIDFLIGLNTAYYNAGSLIIDRLEIFKHQIRKSYGLEWISTLILAAIFIVFKSLEITTINVTQNPSYLILLTVLSHQTSVHYKASEYELALNLSKKASSYLELLKFLLLLFYVIHLFSCLWFWVGNYSRENYQLNWLDSLKDIPILDWTDEYLQSFYYTCVTMFTIGYGDIAPQSGLEKSICIFLILISSIQLPYSINTVGSIIEKITDYGEDTKNKLRTINSYMNKKKVPYYLQNQIRQYLNHYWESIEGQDTEEEKVIISQLSENLREQLIIQANSQIFSKVPLLQKNFSIQFQQNLLKKINSLQLQPEQIIDLDNSDIKFYFVDEGEIDVLMESGQIIQKAKKDDVFGLKNLFIGNIIKNKKLKSVGFTKLLVLSRNDFLQELKDFPNDYEKFCHIKDDLIYNYKSSYIHRLCESCSSQAHEINLCPLLHFIPQKDLIIKRYQYALFQQRKVFCRRPFKSQRLENDQIDNLDFNEIKQQFIKKYHLPQQQQTTSNNINTMSFQNGTPEDFKESKESKIEYQLSSTAQVSLKRDSMLALSQIPGDHIEQIKRTINNHNRLRTNKQINNKQIDYQLDGMGILKQNSLASRRQSQGLNILNIYQLGLNNQNNNNNFKQNYAQELLTSSINEINLDDELKQRYENLKLIKSMNTFDKESIELLYFKQQNLQKYMKKGLTEFEVSKIFHDYYPQHNVDRQLRRINKNNNFQLQDLIKKYISYLQYPAEFIKTYNVSHSYKYGGIRITEEENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X5S0</accession>
    <name>A0A8S1X5S0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1110133</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000111">
        <property type="protein sequence ID" value="CAD8196112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X5S0"/>
    <dbReference type="OrthoDB" id="298840at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052849">
        <property type="entry name" value="MORN_repeat_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46917">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46917:SF1">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="335"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="413"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="343"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8196112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="738" mass="86328" checksum="B2E1D7F94721B771" modified="2022-10-12" version="1">MKEHKDGIYQGGFNAKQQKHGLGIYLWDVGSAYYGEWKNDLIDGQGILFLGGQIIIQGGFSKGAAHGYIVIKVGTRVFYSQFDKGEAIFIQEGERKITIQSSSAYITHDCTHEADCILQGISKLQKGDLQIQLFNHCIYYGNVGKANLPNGLGIEISNLFYQCGQFVEGKLNGLGRLDQQDEIYQGQFQKGIYHGNGLMVFKDQRIQWVKGIYNHGQLVEIKGSGYLDQMETPQELVFKFCKKANQQYIVIPISKEIILGYIRLFEVMQFVRLRVNTLTDIKYQQQPSVRKYSTNTNNLSKSVEEIRKLQSQFNTRNLDQQNQIYEIILDQHDENQTYRKTDTQQSRTKTQPTEEYFENKISFGLLEIPNLKNSRKTLGQQKEEQNLQIKKNNDYIQQRSQSQFEVKQQYQYQQQQQQQYQQQQQQQQQQQQQQQQQQQQQQQQQQQTNDRMLTLKNILEAQQQKYNSDFKQKKTVFNDIQSTTAQKTKQIVQQNNKNLQCLSEVVQREINRSPPSEERRSTIKPLYVQNEENEQKYLSSQKTLPSKFNMEQTNIYNDHNNKTNSLLNKFNSIKIKPNIKQETQNFGFSTKSLSSKQSIQKVQDTNDLPLGNIQNYISLVSHKNNTQVRNLQNSFEKQQSKKMDDSNYHSCNGNCTQIQSEDPNTKYIQEIKQELAEQKQMLTQLFQSLQQKQPQINKQYFKRLPNTPQRNIQMQQQPKQVYVYPTFTLVKSVSPMRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X7C1</accession>
    <name>A0A8S1X7C1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1140035</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000114">
        <property type="protein sequence ID" value="CAD8196945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X7C1"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="118"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="370"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="766"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="823"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8196945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="894" mass="104175" checksum="7A03D80FD2BC5719" modified="2022-10-12" version="1">MQFNWILIKFHSINVLNIVMGSILYLQDISQLGSLILLILNTTIMLIGILILRKKLINQNHLQQLILAILSIHVSYCCFLNRQLNVTTYLLLKFQEAYIIKKQSRIKSILYHIVYNSVLFSLLIFHSENIILYILLCYCTLIMIKKNKSNFQLENQDFELPSHLIKQTQRNQEVMNIVQDTLSNSWMIKLQNIPVGIMIVKKENLQIIFENQSLLQIFEGIKDIQSYLMNELQFKLQIKRMRKQVRESSIIMQKQQNQQQNNNFSQLYSYSKIPSQQKISNTLKNILIELQNGKLDQIYQKENHLELVGQISKQKQLQLFEDENIRKIQCKIFCGQNDQEYFIIVDDISLQSYLQKLETREKFQVRIIDSFSHELRTPLNSAKLFLDALLNDPKIKDTYKTNCIEPAANALKLQAYLIRDIIDFTQYHSHLIKYNIQEFNFENIIQEINDIFKPICLIKNLGLHINVKNSVPALINSDFDRIMQILVNIIQNSIKYSERGLIIVEISCYEKALTFCIKDQGIGIPQNKLQKIQKFLKSYNQSRDISSQDEWEGFGLLVSQMNLLKLAPQNKSQLRITSRGQAEGCQVTFKIRTTQKTNTQIFRGNTLQRTSLKCAFTVPDLCLGIQGILIINNPRQDNSIQQIPIRQQHSVANYFKPQQSTGVLNQSIQQETDLMNDEETERGYPEEQLLKLNSQSKSTNLIVTNFDTNDKQIKKRKQSLFSVCDLDACSSNRPNTLISRLIQREEQEIEMEEQFQNLRFACKCARILSVDDDIFNQKALQVIINQMGFRLQIAYNGQQAIDMIQKTEKCSESCELFYFILMDCQMPIMDGWTTTKVLMDMIRQNIIPDIPIIGLTAFNSTEDIERCLEVGMREVFTKPLNINLLKQVLLKLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X7V6</accession>
    <name>A0A8S1X7V6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1140138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000114">
        <property type="protein sequence ID" value="CAD8197149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X7V6"/>
    <dbReference type="OrthoDB" id="295173at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="250"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8197149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="280" mass="33644" checksum="4DA88805C17B81FC" modified="2022-10-12" version="1">MIKYNSENHFKFHNVKVFDKERMILYFKRMPIEQEENDNLYNGLNQLIMKERIEQTKQKVEEGKEVTSSVKMDQGELPKNRTLIIMNFDSPFDRKFIAKVFKLCGIIRRVYVGSMKVVDNNKKKLLHVGLVVFKNEYDLTKCFDIEYFQQRINNKFKSAPISTNYEKKLLQNYEFVDEKKNQILEDAQKEGYQIAMNKYGDFETNVEKPKFEKRNWVGRNKNKTKKNIDVEEIEQVIQGLDDNLPHQKKKDILKLQKQQLQQQFQQDIENLRQKQKKVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XDJ2</accession>
    <name>A0A8S1XDJ2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1210074</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000121">
        <property type="protein sequence ID" value="CAD8199237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XDJ2"/>
    <dbReference type="OrthoDB" id="308197at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="96"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8199237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="248" mass="29650" checksum="D39E5129CAEB283D" modified="2022-10-12" version="1">MKQQQISTPSTQFGLRHSLQIQSSPNIKLYQQSPYHSPYQSPLLQQQSLSQQRQQIQQNNLVNINRSLFNDAQNKKCKQQQIVDLNNIEEPWRQKVFELQRKIQQLEQQQQNQECEQDESELSQVYSQIQQLVNTIKILQEEIQNLQEKLNIKQNIIDGYDKQLIAKDKELDDQNHYIQELHNQLEEQTLKMDNKQKLLLQEINSWKKKFIEQNKGLHEIQEEQIKIQTQIDNLKNIKLILNKQSEQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XDT4</accession>
    <name>A0A8S1XDT4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pseudouridine synthase I TruA alpha/beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1210124</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000121">
        <property type="protein sequence ID" value="CAD8199327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XDT4"/>
    <dbReference type="OrthoDB" id="10256309at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02568">
        <property type="entry name" value="PseudoU_synth_PUS1_PUS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.580:FF:000029">
        <property type="entry name" value="tRNA pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.660:FF:000002">
        <property type="entry name" value="tRNA pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00171">
        <property type="entry name" value="TruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041708">
        <property type="entry name" value="PUS1/PUS2-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00071">
        <property type="entry name" value="hisT_truA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142:SF4">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pseudouridine synthase I TruA alpha/beta" evidence="3">
        <location>
            <begin position="176"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR641708-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR641708-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8199327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="350" mass="41159" checksum="E796E46310914590" modified="2022-10-12" version="1">MSRNYFKQKVLLTFGYNGANYHGLQIQKGVDFDTVESKLFEALKVSNLIMEQNGNDLSKSGWSRGARTDKGVHALCNSIAVKLCINKEFFKDDVQQNEEEIVSKQKDKAKVDYGKVLNVINSNLPNDIKVHSIKLVTQGFDVRKNARYRFYEYIAPVSLYSEKKGEEILEKVNNLVKKFVGTHNFHNYSRGMKYTDPQSMRYILSISVELFKYEEKEFFKFLIHGQSFIYHQIRKMMGIVIQIFQEELPDTFIDNTFFKNQLRIILAPAEGLFLNRISFEGYNTKFDIPQRLEIEEEDQIKIHQFRPTIVNFICEQEIKNQTFSNWLKILKEKNFNIEEEEDNNKVENDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XDV1</accession>
    <name>A0A8S1XDV1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Trichocyst matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1210029</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000121">
        <property type="protein sequence ID" value="CAD8199155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XDV1"/>
    <dbReference type="OrthoDB" id="300337at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035917584" description="Trichocyst matrix protein" evidence="3">
        <location>
            <begin position="18"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="341"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="54"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="118"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="310"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="341"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8199155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="394" mass="45825" checksum="BB8FE0E07B9C9AC5" modified="2022-10-12" version="1">MQCKLLILLTILTLSFQFDEDIDVHDEIEKLKQSQFGQTILDTVQIQLSGPEPLQNLINMLQNLNTQLEGEQDRDDRLHERYQSQCENDLSALEDIINESTSTSKLLKGQIDQLEPDKQAKLTDLARTENELKELKNELEYQTDKRKEEQVRYEKTLDNLEQGLYALNEAKKMFNTFLNVLIKNQQRFASLIQLENQDKFLNKNYQTNDEDELTLVEIAQQINKIKHNVKAEGINFLLHGISHIVNLLSKDPTAEQESMSRKVLEIISQVDNWIQKQRIYEDLSEQQRKDAFGSLAQMLEDQIILKQENLTFLQGSIESLNSQITSAKNEKAEVDVKIETKTSQNDDRDTECRNEDLDYQSSKNKRDKEREELGLVLDLVINKIGQLKKEILQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XF88</accession>
    <name>A0A8S1XF88_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fungal lipase-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1220148</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000122">
        <property type="protein sequence ID" value="CAD8199787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XF88"/>
    <dbReference type="OrthoDB" id="438440at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051218">
        <property type="entry name" value="Sec_MonoDiacylglyc_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45856">
        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45856:SF25">
        <property type="entry name" value="FUNGAL LIPASE-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035758213" description="Fungal lipase-type domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Fungal lipase-type" evidence="2">
        <location>
            <begin position="86"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8199787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="269" mass="29965" checksum="BCDA44669BB41C07" modified="2022-10-12" version="1">MRSSVLILVVLAATVNCAFVYNEALATEEAALSFAAYCPDTAINTWNVGYVSTNYPNIEKPLVFENTIAGTKGYIAYNPSYNAITVVFRGSSNIQNWLDNIQFDKVNYNTACNCQVHSGFLDAFNSIKPQVDSLFTKYRGLYPKAIIHVTGHSLGAAMATLYTTELAIAGYTVQLSTFGLPRVGDTAYYNYFSSFTKVTHFRVVHEKDAVPHVPPENFGFNHVDREIWYHKSSYTVCQLDEDPNCSDSVLVPSIQDHMSYMGWSSTVDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XGN1</accession>
    <name>A0A8S1XGN1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1230065</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000123">
        <property type="protein sequence ID" value="CAD8199999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XGN1"/>
    <dbReference type="OrthoDB" id="288674at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8199999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="110" mass="13003" checksum="42013E78B5956A3B" modified="2022-10-12" version="1">MNQTQGQNTQAIQNEELIFFDSKGLDMLIKQMGFEEVVQFDEKPKQMIIDIGNEFMNKLLEESMRVSQKRQQQNDELKNTKPKLAFQDVKFALENCLRMPQQNFNIEKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XHK4</accession>
    <name>A0A8S1XHK4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1250060</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000125">
        <property type="protein sequence ID" value="CAD8200646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XHK4"/>
    <dbReference type="OrthoDB" id="421226at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="266"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="295"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="334"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="551"/>
            <end position="642"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8200646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="987" mass="116894" checksum="4734053E0FD66F78" modified="2022-10-12" version="1">MLKNKPILCTEEPIEEDQIFIQAFQGAPNVSVSISEVSESQNDSKKVFEIPQVLIEHHTQEKQREEELLKQQEPKKGFAKLVQLFHMRQFIHQVLAKRRLISKITKYHLQVINDKASSLDDVGFRKFAITIKPLIQSLQENTQAHLGNQQRRSLLMQRTKIFIGSQVQRFGKLIQQIPLIKPDSKVKVVWDSILFICRFYLLFTIPIDLAWPSIQLLFYTLEVPTVICITLLILDFFVCLFQSFYQQGQLVTDRMSILKHQISKAYGFEIIANILLIIFYNISNQYDYGFNVFDNPYYIFLFLQFVQYNNIAQLRQQLEDALNLSREAASLVELSKLFVLLFFVIHVFACLWFWVGLYSETYLGSSWLEAKQVLDKSFADQYLYSFYYSTVTMFTVGYGDITPQTNPEMIVSVIFMSVCSVQLSYSVSTVSSIIEQISGFKEEKKRKFNIINNYMQQKNISYELQFQIREYLNYFWTMKRNEESNEEREIIDELSSRLKQRLIFEANSRILYNSPFFKENFSLNFKKDLVSKITSITIAPENVLDYTKKSQDENSLSIFFIEQGQIEVFIENEQLSELSRVYKLKEGESFGFVSFITGNPSVEKYKTIGFTKLLIITRKDFLDVIKDYPEDYEIFYSFFEDLTFNSESELLTMECFSCKSKTHKAISCPLLHFKPDREKIIKSAQFSNDQQRSKQKKRKEKCYIKATLGQSILNDVAKQVQSDNQQLTYIYEEYDEEKSPTPKVQFFNSSDEISDFKNNHEDEKIQLIEFKEETLKPPIPQKKQLLRKKQTLQDIGDIMKFNNRQKETKEKFISIVKKVNLMNDFVDKKIEDSQEKMLAQYRDHLKMIQYRKNKIQHIKHIYQNKTINELEFFELKLKMLLQTEFDILLDAQKSYKYFKPKENLYSVLSRTKISMLSSTNEMQILMQQKTKQLIKYLFYPYLYIQKYVCKQRHSDYIGETKKKQLFKRNNRISLAFIMQQHSSIKKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XLD3</accession>
    <name>A0A8S1XLD3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DDRGK domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1280064</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000128">
        <property type="protein sequence ID" value="CAD8201538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XLD3"/>
    <dbReference type="OrthoDB" id="2285710at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044389">
        <property type="term" value="F:ubiquitin-like protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019153">
        <property type="entry name" value="DDRGK_dom-contain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050899">
        <property type="entry name" value="DDRGK_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48176">
        <property type="entry name" value="DDRGK DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48176:SF1">
        <property type="entry name" value="DDRGK DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09756">
        <property type="entry name" value="DDRGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01128">
        <property type="entry name" value="DDRGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="45"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8201538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="297" mass="35749" checksum="3E4BC23E39250F1F" modified="2022-10-12" version="1">MNFDIVFLFVSILVMGGSYYLLFVKKKNKQQTVVQQGQQQAQVQQQGQQQQQQQQQQRRQINRVEVESDDESDRELDVQNAEQQAKREARKQKKQQQQQYIRQQIEMLEKKNEKRSKEYEEKERQREQQEQEEEEWRKKIEAEQKLKEQEEFDKWSSMFQVEQSGTMKLSLEEEEQKNKEFNEKLANYIKLRKVVQLEEVSAELSISTTEVVDKIKQLEILGQLNGLIDERGKYIHIRQNEIESLINYINARGRMTRSELLNESNRIIKLEPSKQDLQKIQEEEQKLLNDLQQELQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XMW3</accession>
    <name>A0A8S1XMW3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1310002</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000131">
        <property type="protein sequence ID" value="CAD8202373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XMW3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8202373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="63" mass="7234" checksum="A7D7EEDA82BAE980" modified="2022-10-12" version="1">MPYVLLPTVDWFILVLQRISKDSCTTSTSTVSHMPSYGNCKINQLQYIKLTDDAIKAFKFKSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XQQ4</accession>
    <name>A0A8S1XQQ4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1320157</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000132">
        <property type="protein sequence ID" value="CAD8203008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XQQ4"/>
    <dbReference type="OrthoDB" id="307452at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035918134" evidence="2">
        <location>
            <begin position="19"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8203008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="331" mass="38731" checksum="681AF5471DB8F69C" modified="2022-10-12" version="1">MKIAYLLLCLVVIQGIEFESFQQAIDFDPASIGSPNCQTDDEYTNTYEAFFPWITVLEEGEIEMQEDHSVLSQAKEKIIQAREFIESLDEELQQEKQQESNNQQNESVPQVQDVQTEKVEEAEQVDEDDEEEDPFGWGSGEIPQLLVHKKERKAHKNTHAFIQIMNGNKLSKFKSKISKLISMATEMQDNQDEDGQKRRQRKIELCDEIIDELTDMILFLEKVMNDYDYQNEIYELFKQKQQQIQEVATNCGQRVISYQDVSLIEEKKSQEKKKIQHQIKSKRRNPVSFLQIVIDDEESQQHQLEYQKQILQSQGMLGFLRASGKQVKLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XXI9</accession>
    <name>A0A8S1XXI9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1370040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000137">
        <property type="protein sequence ID" value="CAD8204384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XXI9"/>
    <dbReference type="OrthoDB" id="300569at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8204384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="183" mass="21658" checksum="3992D28CE575F670" modified="2022-10-12" version="1">MKVYEYQKITSNSIGINITLRDLIDQPIQTKTVKVIEVFINEGDYVYNCYIALKILNQGELIELYNRWPGQIDEVYIKENDLIGLDSVIYNIRGDFRQDLQIEYIQYGVINKRNQNFRINIIKNEEEHIPEQGDKIQLDPKMILGHKDNVFKVVMNDGKMNDINFQVMKNKYPHLVVQYLEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XY21</accession>
    <name>A0A8S1XY21_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1440100</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000144">
        <property type="protein sequence ID" value="CAD8206571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XY21"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="142"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="114"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8206571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="487" mass="56618" checksum="EDDA69FEF28BFBFC" modified="2022-10-12" version="1">MLYNSFFKTKNQSPYSGKPKQQMRQSSFEKPKKERLRLQLSSKIDQFEKEAILSVVNSKKKSVSKRFKTEQGIDIIDDFLNLDSYIPKQQSKSRSFSNAFPSNKSADRKQNILNSTSKRQKSNNQKQSLEEDQHPDNFPQQFQKIKLLGKGGFSLVWLGEHKTTKMRVAIKQIKQSNSNQAYLREIWFGSHFFDNGNPKPQFKSSIGVKSLLQYLGYEIGTTDTWIFTEVCGESLKNTLYDLKGQQIKNEIVFKMIQKPLYLSLKSDLNILKKIIKDVAHALILLSEQRIVHCDLKTENILIKKSKCLNGSYIITQTKLIDYGSSFLFDDLSQFSMATPEYMCPEILNYIQYENGKDYDAKLFKVLSNYYKPWVIDIWSLGCVILEIVYGIPLWLGNKIIVKHHDKEVIEQGLFAVKNRAFDQIIQKQAHVVKNLDHYLDNNYSGIKVDLEIRLLLREMLTIDPDKRIAPHTIIEFLQPTKERLRTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y0V7</accession>
    <name>A0A8S1Y0V7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1400070</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000140">
        <property type="protein sequence ID" value="CAD8205274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y0V7"/>
    <dbReference type="OrthoDB" id="295747at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="147"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="207"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="10"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8205274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="357" mass="42972" checksum="22E9D89BA194871E" modified="2022-10-12" version="1">MSDLKKTKKPNLSSVNLTEKQSIMKQQKSPKHYKMSFDPEKYVEREDYIKMEKKYNELVQKIQTLFLEINNRYYDFNDINEKLNRKSNEFGYTRLDPHRLIELIKEMLATYIKGIIQTEIEVQQYKLCNDDEQQDYIWLLHKSEMDMRKMIRENQQLKLLIQNLEIKISNNEKQIELLTTYTQKAMEEMKSEFTFAIDLLSERESSLNILKDEIKRQTELITSQKQQLISVQVLEHKMGHLAEKQNKEKQKIKQEYDHTVKQKEQYLKQSFELDQRAKDKRIQQLEQEVKQLQIEKNIIERDLAQKIEFSQKKIDNQNNQLAEKISQISDLQKKLIDVHHNNYLQDKANHQSTKNQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y0V9</accession>
    <name>A0A8S1Y0V9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPENT_87.1.T1430119</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y0V9"/>
    <dbReference type="OrthoDB" id="289272at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000210">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000524">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="47"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="335"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="346"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8206278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="372" mass="42619" checksum="425B5FB64395F6F0" modified="2022-10-12" version="1">MGNMCETDSSPTRISSLDKFEVGPVFDESPTIQSASPKRRKVNRDDFINLGLIGKGAFGSVYKIMKKDNGQIYAMKELKKQTLSEHNLETVNFLERFVLKSSKCPFIVKLRYAFQTSSRCYFVMDYVSGGDLHRVLKKNGALPEKIVKFLIAEIILALEYLHLELKIIYRDLKPENILLTETGHIKLTDFGLATYIKNEHTYTIAGTPEYLAPEIIARSGHSYEVDFWTLGILIYEMLTGQAPFTCEDRNVCTIQNLIMQNKPIYSPKISSNARNLISNLLRFNPKERLGAKSFDDLKRHNFFQNIDFMALSQCQLLSPLETVAQKNKLEQEQSLSPIKNEDRHQNSPLNCQPINDFTYDPHLDNEINFQKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y282</accession>
    <name>A0A8S1Y282_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1450041</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CFAP97 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000145">
        <property type="protein sequence ID" value="CAD8206758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y282"/>
    <dbReference type="OrthoDB" id="292876at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038791">
        <property type="entry name" value="Cfap97/Hemingway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029488">
        <property type="entry name" value="Hmw/CFAP97"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23035">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 97-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23035:SF2">
        <property type="entry name" value="KIAA1430 HOMOLOGUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13879">
        <property type="entry name" value="Hmw_CFAP97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="402"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="451"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="180"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="255"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="182"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="214"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="286"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8206758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="473" mass="57176" checksum="D4863F7304C03EFE" modified="2022-10-12" version="1">MNRNIPIGNKICATKEIFQNQVILNSHLLTMRPQINTSAPKQYDFLKNKKIKEAIKSQKQQEIDRENQNLISKLTNIMQQQSNNSISTLAIKKSTTVSLGPKKSLNKEYRKKELIKIVMENQQLLKRIQDQKSQYNVKDWNQERRWVEKYISNICEYPYKDFKPTKTLVQYWTNSRISESQLQKRDNFNNKKLDPLEIKSQQTKSRARQENNTESRQILINQENQQRSLFESDFQPYEPYQDKVSKIVNEIIEKPQQQEQEQKKEDNEVVNDQKNQEKPTMQEDQNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTMKLITQWRIHKQMKSNKIKVLINLLKIIKIYKPQILNNRIKKFWTIKILNNSKIEYFYTYCKIIIYIKQFQGHQQQKYNRLSNIYDIAYMLDIFIIFWIAACIHYQQYYTYSIMFQKLIQLKIHIFFTRQKDDRRYSFFFFSNQLLFCSILYQISRQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y2P0</accession>
    <name>A0A8S1Y2P0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1490033</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000149">
        <property type="protein sequence ID" value="CAD8207920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y2P0"/>
    <dbReference type="OrthoDB" id="300858at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035826582" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="16"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8207920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="134" mass="16126" checksum="44071B8A737BFE7B" modified="2022-10-12" version="1">MLLLTLQFLIYFCNCFNEEEQSTLTNPIKEYHLIECEADDQCNRCNYIQMNEIKQCQVSRQIQRMICYYQLGDDEQIRYPQIDYKACNLHKIGIFSSEFFIVVIIFAVTLFSWYVVSKEKDETEKQLLYKILRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y6I2</accession>
    <name>A0A8S1Y6I2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1550085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000155">
        <property type="protein sequence ID" value="CAD8209635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y6I2"/>
    <dbReference type="OrthoDB" id="429991at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051291">
        <property type="entry name" value="CIMAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580:SF28">
        <property type="entry name" value="BOREALIN N-TERMINAL DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580">
        <property type="entry name" value="SHIPPO-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="277"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8209635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="414" mass="45210" checksum="6AA97F374BFB4816" modified="2022-10-12" version="1">MVSAYSVGNGKRSNEAQQGPDTPGPGQYSGDKKQKYTPPSYKIPQAQRQTFQSTFTPGPGAYASSDNLLKGPKFSFAGKKQQSVTSFQPGPGAYNVKPTKQAPLYSFSGKYGTHPDDQQPGPGEYQLSKQLDAPTMKFPRSKRDDPHSEKVPGPGAYKQDRPESAPKYRFGNSQRRGLYDNELGKVPGPGQYNYQSQFESVQTKGFTLVSRKEQITQQLVVPGPGAYDPQQIKRPPSCKIGKSMRGLQFANPNPGPGEYEPQLDTIRPQSAMVRVGSATRRPLNDVKGVPGPGTYDLPSKMVEGPQVKILGHKYDPVQAQKDQIPGPGQYERPIMQRPQSAKIGTSRRQELNSTLEVPGPGQYKPKDKLGGPSWGFGTSKRPPLNPKNETPGPGGYDQGNEFGSLPKYAMKKIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y7C3</accession>
    <name>A0A8S1Y7C3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1550123</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000155">
        <property type="protein sequence ID" value="CAD8209709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y7C3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8209709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="53" mass="6027" checksum="CBA0DAB11BAE7574" modified="2022-10-12" version="1">MISVICFLKSIVEFEDLKHFLINEIFISSCSIIINVLVTQVATHPFKQVSESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y9X1</accession>
    <name>A0A8S1Y9X1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1510099</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000151">
        <property type="protein sequence ID" value="CAD8208632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y9X1"/>
    <dbReference type="OrthoDB" id="283082at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8208632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="153" mass="17467" checksum="071A6381B7D74369" modified="2022-10-12" version="1">MFQAEKGANAIPGYTGFIPSQDYDQDLLQIQGNRNHIPNYAGFVPGIRSENLFGKTFGKITLLSSTHEHHRGSDLPADIRYKSEVKEAYCDQREQRGRDINLYGKLDPETSKALSYTLSNLTHFEQTNILPKRCDSKERQSNLTYEEALQKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y9Z2</accession>
    <name>A0A8S1Y9Z2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1510109</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000151">
        <property type="protein sequence ID" value="CAD8208652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y9Z2"/>
    <dbReference type="OrthoDB" id="292236at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8208652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="479" mass="56707" checksum="38E6785039F34658" modified="2022-10-12" version="1">MNKFTLKFDDKILEEQYQKVKLSKIRKPIYLGLLLLCFCMMSSNAILSLFTSQQQTLKTYINYSYLIFMVFQAIIVYKRPHLIKYLLAISNIATGLLQFNFNESTPAQIYYDQSSSFTQLQAVAYFISDFKDGVIQVVALLFMRFMITSIKIENVDLQTVLIGIASTSFILVTLYVNDQNSRKEFLGSISEDLWDHSLSILIKKPHFRVQYNKEYMKIDLISSNQLCDFPGYDENLCEGCNSRQLLHLYQYNKYNLSDFILEQAQTIQNQIKVQYKHHSFQLRIVCVGIKNLNIVVIYEKTTSNVNEQIVPIKLRNLIFQYIQSQSQKTIYQRLFNQGLLSILLINKMKLKEVEITKMFKKLNKQFKYTMKPIKILPKNINFKFYTYSSQLKILLVQIFQIFDQISKKRISILFQDLEDCILMQIKFKQSQERNLNLLFLSFYSKNIFIQQIKSLLLFNELNLDLQIQFKKHIPYQLIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YAG5</accession>
    <name>A0A8S1YAG5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1510012</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000151">
        <property type="protein sequence ID" value="CAD8208464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YAG5"/>
    <dbReference type="OrthoDB" id="285163at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="138"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="561"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="561"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8208464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="583" mass="67158" checksum="B6B7A13E545A0024" modified="2022-10-12" version="1">MTEQKVISIFDFGKTRNKFWQTCDKSVLDSQTFLIEQEFLTAGKAIVKPIFIVLGLEYIYKVTVDKLKCAPLITTHLTYIEPGSDHLVQLNSDEQQYGFRLQYQTKTLEIFLSDKSRYDQWRIHLRKACLLENFHEAYMVSKLIGKGSFAKVYLATRKENNIQYAVKAFSKTFMLQQHKGIESLLNEMKVMRKFNHPNILKLHEVHETANSVYFVVDIVAGGELLQRVRETGFLPAETLQRLAYNLLSALNHMHQFNIAHRDLKPENLLLKSYENNYEIVLADFGLAALLQDNNILFKRCGTPGFVAPEILDYIDGQQIYDEKCDVFSAGIILYILITGDQPFTGKDQKAILKANKDCIINFDDSLFKSAPIQMQDLIRSMLLKKNQDRLSSTECLRHPYFKELAKEQQVKQEKYQQNLNDYNQYYKNNVKMGSIDLNLEQRQPAFTGNLNSIESISCVSNNSFAKIEMKPPSVIGASKFSQYSCKLNRLGSRDISDIPTTQLQKTESKKHIDLHRIAIQNSHRKQIQDQFEDQPIQNEGSGINEMARMYNSTRQIRIPENLQTVSQPSKLASTPTNRNKKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YAS7</accession>
    <name>A0A8S1YAS7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1570015</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000157">
        <property type="protein sequence ID" value="CAD8209917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YAS7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8209917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="128" mass="14664" checksum="04690FB9541DE8C7" modified="2022-10-12" version="1">MAKTSIELKQVQYTIHIQPGLAAVQICQIYSTKQNKDQSQLEYLFKIDQNSAVSKNDQSLWSNKRVRRGQKKYQKGIEDGKTIVISQEDQEISNIKKAKIGCLSPGKLLKNQFVHIQPLQVFLNQFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YBH0</accession>
    <name>A0A8S1YBH0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1590123</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000159">
        <property type="protein sequence ID" value="CAD8210548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YBH0"/>
    <dbReference type="OrthoDB" id="312008at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8210548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="359" mass="42396" checksum="76A2F3D1235A77AC" modified="2022-10-12" version="1">MQLILLMHQVASNWRLVDQSFTELQMVSNNWEINDRCIDSSERSINYQTCTQNYIQYVLLDDNRRYMYKSFNYKSYQAQVIFDAYFDDADNDIDSSLKVSYDSNKDSKSEQLLYRRNYKQSDLNQNSVRICHSTWRDFEFYTVVSTIANSNTNQFKIKICFNPRRSCVSLGIKNLLIYINSCHPTCLTCNGPTETQCLSCFGNQSVQGGKCYCIPDQQFSETYIGCLQECSRDYSIADYDKICVNDKSIRSKFTLFESESIPQSNQRYYPLIFEKDEFNPRNSDLIMKIAMGLVLLESYILMKDFFTIKFIRIRITFYFFNFQETSTIYILHNNRIQSRIIKGSSDFQVDYLVKIFEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YGC9</accession>
    <name>A0A8S1YGC9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1810002</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000181">
        <property type="protein sequence ID" value="CAD8213556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YGC9"/>
    <dbReference type="OrthoDB" id="326948at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026306">
        <property type="entry name" value="RSBN1/Dpy-2/CEP530"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13354:SF11">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13354">
        <property type="entry name" value="ROUND SPERMATID BASIC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="400"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="408"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8213556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="719" mass="82314" checksum="C846CDB713A3F4A8" modified="2022-10-12" version="1">MVAFNIGEGSFRPGNEFRAKNDAFNILIGSKQLLGKQLDDQVKEKDDGDYDNGITITSSTDDNKPQIDETKESQEYLAEYGYGFWARFLTAYPVRLLDGKNAPQYFISRLTSNSNYGNIAMGGRTLAIWLGQGFYQFTTCDKKTNNPNVMQNIDFQNDIEGVCQGLMVYSVKSLLDTEDDFHNHISKTIIPVHDLDGQYQHELVEDTISRKVNENPIYDELGGGAQSFPYEYAISGWFRWEKTQQEVWHNFFEVQISKPSTDKFLGDRTLSFWISTAQGGILHFPTYTYTNMNGAGDPNLGGNIFHRDRHLEWFFKKGFVGVKFTGGIETIEYNNVNHYYTPYFYRFIGKDKQNPGFNGKIGYVSFVLGSGTFRKTPFFKHLKDIVGFEKGQSTLVIKRDQKKRQPITDETGQQQFPSAYNQNSPQMVEEFRSEVSFTEYGYGFWAKFLTAYPVKLPNGKNASWYFMARLTQHEYYENIRMGNRILTIFQGQGYYHFATINIQTDDPVIRNINYPADIEGIWTHFYFSYSNTGKAVAIVKFGELDPVLAEFPVKHPDLKYLKFIFGGKDQNIYPGFNGQFTQVFYSTSVGAFIDSVDSYNKFLADKTSPLKGGKVSLSTTRIIDSQIDRQVNSDPFKTTVETIFPKEYAFFGWFKWTRPTTLSPWHNLFRVQINTPSTDNDLGDRTLAGWVGNGGIIYLATYSYRNMNGDGLNNLPQEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YHH7</accession>
    <name>A0A8S1YHH7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1940018</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000194">
        <property type="protein sequence ID" value="CAD8214046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YHH7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8214046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="208" mass="25235" checksum="7EDF330DF585F85F" modified="2022-10-12" version="1">MYQKDNQFNHKIGVIFYYQSYFYIHNIFHRIFVKIQKSLLVSDFRKLDQQNDLNYTEKGIVKMYIIDIYIPHVNKQKITFQMAKYSDKNINYTFNLKDYMEFVKQCLLMESCESGKKMIKMHVNQLKINVIRIKINFYVKHIHIKLILLKQEVVRILKLGIIRKFIYVTIINNNVFQWILILLNQDIVTQRQLSLIDGIQIQVHVFFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YHI3</accession>
    <name>A0A8S1YHI3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1740042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000174">
        <property type="protein sequence ID" value="CAD8213159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YHI3"/>
    <dbReference type="OrthoDB" id="305664at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="53"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8213159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="271" mass="32001" checksum="A94A0CF04DD4D3DA" modified="2022-10-12" version="1">MMQQIQVLNVNSNLVFTLNFMQQSNQLISGHSGGSIVIWSRNNNYQWNSSQTINGHNDYINCVILNKNEDLFISSSSDKTIKFWIKQNEWICQQTITDHTNDVYQISLNDQQDKVISCGMDNNILVIEYSEQSKRWIVIQNIYVDCFGWRLCFINNNLFTFQPFNGNLMHIYEMNSVSKQFSSSKDITVNQDQDGWSLFPQQFIKQKQILVSKHSGYVNFIRKTEKDEFKVEQSIQFNTYTLFGQLSDDGEYFITWDDQSKEIQIRKYREE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RX35</accession>
    <name>A0A8S1RX35_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0020492</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RX35"/>
    <dbReference type="OrthoDB" id="433924at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="1">
        <location>
            <begin position="8"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8133611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="312" mass="37558" checksum="D1D0C767DA621360" modified="2022-10-12" version="1">MDNNEEHYEVEYVFGKRLDKGNIVDYAVKWLGYDKKHHTWEPISSFSAASLLLIGRFERYLAYKNYYKLIAKKVEKVKQPKKPDEPIRVHIRNRFTQVLIKEAEIINIELDTDEIVQKKETFQINDDDDDDVQIVSVNFKPIKQNESKDLNRFKVQKVSQTLDFNRMMHIRQQQFNPIKINTYKSKYKVEKDYLNEIIFEQRQKEKEVNMLRFTEINDKFEPSQPIKLISDYTQTEGFSTQQNVYRNQQFQHRVFNQKVPIEFIQSHNLIQNELWFKCQSDEQEILFLDLQALQQNYSTLLLDYLNAFSVMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S1G1</accession>
    <name>A0A8S1S1G1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphatidylinositol-4-phosphate 5-kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0030091</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S1G1"/>
    <dbReference type="OrthoDB" id="2129491at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016308">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00139">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023610">
        <property type="entry name" value="PInositol-4/5-P-5/4-kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086:SF8">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 5-PHOSPHATE 4-KINASE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="68"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="98"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="149"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="PIPK" evidence="3">
        <location>
            <begin position="329"/>
            <end position="674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8134016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="682" mass="80595" checksum="54D3FFF7A295A42D" modified="2022-10-12" version="1">MDCLKFRKEKFYQQTLLNTNETRFSDKELNVLRKRFIYMTNGSKYINKEQFKSNLSILGLESISCLGDALFQAMDINTDGHLEFEEFVAYFDKITYGTQEEKAEISFRIIDQARKGYITYQDFSCTMQQLIKSYTIMKGTDLTDDILEHMEKRYQEIFNLIDMNKDQKISLQEYVLALTRNPQILDIFEFLKKGVTQTIKESQHKRDQMMLNEFDKLRNQTAQILNFIQDQQEKSISLVKKQSYNSKYGVDVLFDEAEVSENLIEKDDQISIQSMSNDQLKLKFKEVYQQLQKLYLDTENSLSKMESLYHEKLDQQEFQEQVLIKQVSINNNKYKKSSVQISNDIWDLVINMMVGIQMAVKSSNAVIENFISNTDFELKQCFEIISHQNRDKCKFYDYAPKVFNQIRKLQMIDNDNYLNSIGPENLLFNFMQGDLSTLTELTSTGKSGSFFYYSQDGLYTIKTISEKEFYLFKKILINYFNYLKDSPNTLIIKLYGLHKISINGKETCFIVMGNIFKTEYQIHKKYDIKGSKYKRSTQLNVDPDVARKDLDFISNNEIISIDKKRRQELIEQIEKDSEFLSNNNLLDYSLLIGIHFIESKKEFQMIENLRDFKIIKSIEEDKLFFFGIIDILTEFNTQKQLESCFKRIIQGPQISAIPPKEYSLRFKNFIRQIVSYQNNDDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S3Q2</accession>
    <name>A0A8S1S3Q2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0030205</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S3Q2"/>
    <dbReference type="OrthoDB" id="184675at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03213">
        <property type="entry name" value="ABCG_EPDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001495">
        <property type="entry name" value="ATP-binding cassette protein subfamily G, member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF139">
        <property type="entry name" value="PROTEIN SCARLET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="415"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="466"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="496"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="525"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="42"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8134242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="869" mass="99448" checksum="285195A1671F9E30" modified="2022-10-12" version="1">MTDKLQTIGNREEQLVNTDNRAEFSLGKFTPIELAWDKLIINATIKVGKTTTEKCLLNNLKGVMKPAHFTAILGPSGSGKTTLLNFLSGRLISDNLKISGELSLNGKRINDIDKFNDQMAYVMQDDILLATFSPREAFYFSANMRLTISAEEKAQRVEALIRELGITKCADTRVGNTQIRGVSGGERKRASIGVELLTNPSLIFLDEPTTGLDSSTALQVIDLLKRLAKNGRTIVSTIHQPSSEIFNNFDRLMLLVRGNIIYQGDAEQAIDYFATMGYQCPNFSNPSDYFMKLMNEEGLLVEKIQAGESDDFDDAQIKAEFEQRLEGFIQNYKNSNMIKDLETRETALIKENDVGFHIGFIQQFVLIYQRSFLNEIRNPMDVKLKIFQSIVNAIMLMLVYSDLGKYNEGLQNRFGALFFICTANAFGGIQGALHTFSMERPLFLRERINKTYSVHSFFWARSLAEFPFQIIYPSLCVIIVYYVIGLSDENVGKFFMLIFIQFLTYQYAVSYGLLLSTIIPKIEVATALVPALVIPFMILGGFFVNQDNIPYIFYPFTYLSMFKYGFEAAVINEFDNVKVVIQLRCYQQHQQNGNVAIYQLDQLQELECLPIWHFIQYQVQKNLNYNHLKACKQIINPNDFFFVFLYNYLFLNMIKKKQLESFLQQVPDFEGKPNWNLEQHMTPPSFASEIIQLILNEESLENLVCADFGCGTGMLTAGLLCCNVAHVCAYEFDEKVAQETLQTLQEMHDGAFDLIITNIKHHKFPSHKVDLVLMNPPFGTKEANIDTVFLLQAFEHSSGNVYSIHKSSTRQYLEKLAIENKRTFKVLKEFEFPLPKKFCKYHKKDLAFTQVDFIKFGYVKQQEGKQEIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S4U8</accession>
    <name>A0A8S1S4U8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0030399</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S4U8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8134622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="158" mass="18741" checksum="0951655971245B80" modified="2022-10-12" version="1">MIMLHSSRSQSNKKRLSQQSSLINITELNKSRIQNSIILNKFNHIFGSIKSYDIEKEFFIYPPDLKDQQVDDFEILAFDEEFQKLSVKNQNYTKGALQCIQDILHMIFTQSSLFYKIITILKTQGSFNWGKQINFYRYHSQKLFTKIEIRRGVLMTQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S7B2</accession>
    <name>A0A8S1S7B2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0050002</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="299828at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34894:SF5">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34894">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE RSMI, CONSERVED SITE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="1120"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="654"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="758"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="827"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="870"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="315"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="584"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="656"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="668"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="686"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="765"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="870"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="882"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="907"/>
            <end position="921"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8136146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1318" mass="154313" checksum="DA883B1AC92056A7" modified="2022-10-12" version="1">MEQLKFRMLKPILSQRKSTDLSNKRIKQDSLQSINIINSPKPFSNISYRKNHKHKALSQGTYYGSPLLGQNQLEAIDKMKSQQRNDFYGQLKSCDPYQYPTRLQELQLSPKSPREPTVEVIIPSELKEPPITLGRLSDNLSRHNARDKLHRGIPQKHDIKQLASWVDLMVLQIIDKYGDQRQNEFYEEVQNVLTLCLKELIRQISIDCIERSVLLEKIWAQYVEINSSVIKQTLDEKREIEKSHLKQIMKTHQLYQVEIDTFQLIIKNFKEDQQQLTERLIKLKDNGKYLKKTNKHLQNISRELRFQLNDVTTTNKYLLNEVENLKQYIEEAEKDMQNYQQQFKNHLIQQRIQSPSSPRKIPMNRSGTVFLSSRPPEQLKIIRAGKHKTLFHDPKPEEKVQRALKSDTDSSIASDIENMLKNQFTETDDLNSLMEIQKEQSTQAYAPVSQFNYKALLLPDTRHQIMQTDSSLYNISFKETQTELQTDMVEVIKTEEEQLMLIQDQLMKVSANYKSIQGESSNDESPRSRRISQFFQSLGEASSKMKQTVYMQREIRTSLLLVNSAQKDENNRKQDQIIVLKSINDSLSKNIDEATNEIQELEAQLDLQEKINTRLEKRYNKIKERKQQIIEKTRNLVNSFTKTQKFTNIMKKKIQEKKGGQPEARKISSLTTSQIPSQTDQHQEEQQYQNQISQQKINQKQQQNQKKINQEQLHNNSLTNQQLENSRISVGSMTSNQLAISQEGFTKKKWNIHITNADEQKQISDNESDYSQSQQSVQRSTYSVQDDFQDSISQRSLATNRSNISQVKISNKPISNYQLVPNNNHQVSKSAKVKKDKNPNSGYQNHLPLESIKMISETLRQFINEELENSDTSSVDSSFSSKLEDLNKRKLEGSQRRKNVTLPQAPKFQNRNNAESTSQINLQKSQNLVSQLSEKFSRNPKSSNAKMQKINVLKFISQFYCEKIRQFSNKNMPLHQICYEHFVNTYGFKSMAEQKLTNFYQSIFAYRENFRINLFGRFAELFSPLTLDDLEIYIQSLKNLDENEMPIQLAQVVNEKGVLISLDRALACLETQHSCLPLESKDRIQKEIKTHTYERKNKICVDLDFFISKILTGYQQLKMMHQAHFEEVFYSADMDMDGMIEFQEFQKLFLHFEINQGTNLNQKIIRSQFMERCDTVSNESGEKAMSFNRFVTFSLENNIFSKEKFQRFSKNVDLNDPIKNLNDLKENWINIQALLNYRINLSESGETQYYLSIIQKLDQALNNKELKESYWISYRILDADTKAVYLSRLVASYLPQELLCIQELMNEIFDENIQYISD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SAS8</accession>
    <name>A0A8S1SAS8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cytochrome c oxidase copper chaperone</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0050097</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SAS8"/>
    <dbReference type="OrthoDB" id="1915887at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016531">
        <property type="term" value="F:copper chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1130:FF:000006">
        <property type="entry name" value="Cytochrome c oxidase copper chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007745">
        <property type="entry name" value="Cyt_c_oxidase_Cu-chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16719">
        <property type="entry name" value="CYTOCHROME C OXIDASE COPPER CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16719:SF0">
        <property type="entry name" value="CYTOCHROME C OXIDASE COPPER CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05051">
        <property type="entry name" value="COX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR607745-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8136332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="57" mass="6594" checksum="A77DCFF578F4B120" modified="2022-10-12" version="1">MDNSKSKDNQKVEPLRPCCACPETRKLRDECIVSLGEEKCQKEIENHKLCLKKLGFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SB24</accession>
    <name>A0A8S1SB24_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0050291</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SB24"/>
    <dbReference type="OrthoDB" id="297639at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="16"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="141"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8136717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="271" mass="32336" checksum="7A34453299007034" modified="2022-10-12" version="1">MNECISIKSLDKILQQRQPQKQNSYTPQSLLTPNTSPNSRLRVLSPTNKLDSTDKLYRTSNSIKYFQHMNKRDILQKKMNLLTAKLKTNKTEVIKEDNVFSDRLLITSFENFVNETDQKLQKLCYWIGKSPQNNQNLQPIIVNNEEQIENFEQKIIKTKKNLQEQRQQLKYAKQKYQSNKQQLQQLKFELDQLNEDQKILEISTNNRSLKDEIVYNRQSILKHKQILLTVDQFINKYLKEQLLNQNCQIGEYINKFNDIYMQMQITLDQQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SBC0</accession>
    <name>A0A8S1SBC0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0050157</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SBC0"/>
    <dbReference type="OrthoDB" id="448484at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052394">
        <property type="entry name" value="LRR-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8136452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="374" mass="43835" checksum="37C55D1BFBA84C2D" modified="2022-10-12" version="1">MDQTHKSIITEILPNTKRTLERRQKQLEEEQKKKKEQETQQKEQAEKQKKFQQYLIEQERIKKQEEDRKKEEEKRSQEEIQQMNTLKGEYGNMMIDLATGDSKLDYTISGLDLRPTQIRVLVKVTENNNTLKGLSMSRKRIGDEEGQEIAASLEKNMVLERLELEGNNLGPKTLVSISKLLEINKSIRVIDLENNNLTNHDKDRNTYDYSGIYALCEAIEKNDTLLQLNLCNCKLDEKCGEALANALRDNTSLICLDITENPKMNLNDVRKCQDYLIRNKKIYDDERYREFQERQRIRNEDQTSTIQMQDKEKTITAQEGIEQRMNARRLELEQNWKEELEKEAKLKEKTVHNLMKQAKLLGKKKGKGGGAKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SE60</accession>
    <name>A0A8S1SE60_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0060425</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8138050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SE60"/>
    <dbReference type="OrthoDB" id="295274at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14811">
        <property type="entry name" value="bZIP_u2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47416:SF8">
        <property type="entry name" value="BASIC-LEUCINE ZIPPER TRANSCRIPTION FACTOR E-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47416">
        <property type="entry name" value="BASIC-LEUCINE ZIPPER TRANSCRIPTION FACTOR F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="8">
        <location>
            <begin position="106"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="94"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="106"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8138050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="391" mass="46120" checksum="2708498ADEF029FD" modified="2022-10-12" version="1">MQKLNEYQEYHLPEQFDEDQVRGRFLMPSFLPYSLSKQDVKLEDRSMVQIDSQASIKDDALGLQNIKGHVKNAYIKKIHSLLSVEGENEKIVKKIQKEPSSSSKQLENEDQKMIRNRESARNSRQRKKLYINLLEKKVEDLNQAIEQLRKSTESTFQSIHSVLEQNSSIYDMIVEQTQLFEQLKIKEQEFNELQQLLTESYKLKFNATGTKRKQYIRYCFQNIARLLLDGNYGTLLFGQSYFSKTYTIHDEEELTDYMKNLRDATGICDDKVFLNLYAIVRRVIDHKKNFSFLIKQIKLKQKELRICQQQIDDQIDDIQLNGNQFANLIHQVNKDQSNQTQPLEIPQQKQMILKMVPKYPFELMNNPFLLPNSLMIDPLQIDLLAKNLRQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SE63</accession>
    <name>A0A8S1SE63_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0060265</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6127067at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009190">
        <property type="term" value="P:cyclic nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627:SF12">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1118"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1195"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1227"/>
            <end position="1246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1258"/>
            <end position="1277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1289"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1427"/>
            <end position="1446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1452"/>
            <end position="1471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1492"/>
            <end position="1512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1542"/>
            <end position="1558"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1579"/>
            <end position="1597"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2085"/>
            <end position="2104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2116"/>
            <end position="2137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2169"/>
            <end position="2186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2193"/>
            <end position="2211"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="9">
        <location>
            <begin position="1655"/>
            <end position="1800"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="9">
        <location>
            <begin position="2265"/>
            <end position="2396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="524"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="543"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="555"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="567"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8137747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2459" mass="287452" checksum="A2CC749D3238C46C" modified="2022-10-12" version="1">MQRSYQEQVNQKDYQHKLYFKNGYPQNQQFKRDPNQLLRGLVQNQQDNQVVKTCLLHFIDWLKQGFNLWLFIVIILSASFRAEQQLFMCIIQLAGNLFMSSYKLYQQICKALRQVNEYFNQTVYVIGWDPKCHISKCPKRNVTVGNLIFLKRHQPSPMSILILNSQEEKFMVQTSEKEGMTHMTQKQSIKLTSTSDQQKNQTLIYYKRIMTGKIEFDQLDTNDNQFRGFIKLQKDPKGEYLDYRNIVNQSSMIINTDWVVGIVISHDDEDVIKMWHNCFKKSTTHFDRYLMKFSRIFSLLYFIQLAFCITIIIRFQKIPQDFTIYNTLCRIFQSITFVHPCIITGLCELTFQFFNNIFEKNKNIIISHSSELLTLTKIDHCIYDKTGTLTNNNTVLCGVICGVYFYNLINGEGIRNSTKKYRKLMTTQHQHNNMQEIQEEQEEQEENILGIASLQKSERMIQNPGSHHSVKSRLQSQHDEAISFNQDDAVFNEEEPESRNMVFNFNPHFIRQQDQIRQVKAQSIDEEISSSEKPIQGNRNNRRTFNNNSRLITRTRREPRIIERNLEQTQEAGNGSLLSPSPVTSQKDIRKEVKLCESQINEDEQQQLSESDSEDQDQEGRKDLLEIIRLGEKQADNFMRSLILCQDVKTKYSKPQNKPNRDKNSQLMGISGIMNEHPSMNPQNFLNENTEEYQESVIKFAKQYQFEFNCAGLLNKRICYEISAKGLSERYLVYKKVSDSDTFAIFMKPIQQRLRNQGAQYDRQGTERSQKGAQLKLYVKSENLGLLDKCKLSQKKRVNLEKQITHQAQSGYRFIVYAGKDVPEEAVKNKNKYQEVSFDTMEMTHHDKELIRDLDYYGLICLKEDQNPGAYAQVKTFKNLGISQWILSGDKKMQTLSAANHVGIVNNNNTLLRIDEEDEEKLKLQIKSQLLFIRKQLLKEDESQQNESTKSFISQKSFSEMKRSGISKKATFDENMILKSKKGEEPLQKVFLLVNGKSIKPIFSDDYTKSHFLFIALILKTVIAYDCSPADKELITKAVAKIMEPSNTLLTISDSVDDFNMNSHANATIQFSREKRSLKIAYQNIQVNDLQHIKQLVLYFAKLLPMHLSQLTVMCTKISLYQLIIFTLFVCVRRTLYIEEHVVYLLALQIHIIINGLLPSFEDRSSHEQIELMLPQYQPQAYKCFKSNFRWLQELFYEVVMSAAISGFMNTYIFAEFMMFFTSDPNNLAHLMFLFQLFCVFAFGTSQMIHLNVPFGKMIWNFIITLGLLTISFAIVVESDQRVQIIRNIFKWQFIIGILTGVVIIITIDQIIKQTRDLMFTPRIILNFVKYLENAMKNQSGKIVQESVKQFQMLNHIKINRQFFRKIKKVFENVSIDPFIRSLFESEKTQSFNGEYSKISLVFKNHNYEKQFRQFKVQYYYQQRREVVLVLFIVLRVSSVIAWHPFSTVEDTILNILWAIFMVIWILILFVTKIVEKKQVDLASFQIKWGTIEAYFFIPVVLILDHFLSVTLPDDKSQYYFSTFGQLLYQQFLAWDIFPPPLYYPIILTLIFFINYTIRVANFYDIQVNSDQETPTFRVEFITLYVLLFFLNLLSNYTHYNFQRFQRLVFLFDQALQTQQEQTNQILKSLLPAFIYEKIEQSEETEIEENQGSISIIFLEICEFDKILQSKQRQVVTFLDDIFRVLDKICLQYGVQKIETVGKTYMACSGLKSTEIENNKDGLNQQKKNETVIALELSLDFLKAVSEFRYEYKDQDVVKLFKLKIKIGIHYGQVIAGVIGYHKPQFSLIGDTVNTASRMCSTGEVGRITISNEAYERVKDTEFLFTTRQVVAKGKGMLTVHQVTQRKLQIKKHYSQQKYYEGQKNGRQLTFGVNRTNNSRNTQLKQTFKDISPPSSVHDHLTTKEYGRRSKIMNHPMQGSQLLKIKNKNKGAEPTRGMKKATIKEEYSSLTEIVWRDRKSKSQVIQKTDYNTNDWEETKEDEIQMKQVEKDPENRIIGVSDNDIDGPETEVKSERVKKVMDLEILKYKKLTLEYEKCNDANIVMQFEQYYQEVRSALKDIFYPMTIVYTLFKAQIQLIDSGQDGLTIYIVRMFLSLYLIILYFITSRLKKYNMHNLDLALTGLYYVSAFLTMFFVTFNNQQRFLISDNAEAYFFAFSAVNQQSLKFKYMFFYILLQQAAWFVQLLLHQHNNLLVFIVLSTIILVIVSHNNFELTIKNFNSAANENLKKEQRDSLLTNLLPSHILTRFYQNSKIKLELSDVLIDATLLFADISGFTAYSAKVSAEQVVKMLRELMTSFDKECLQQNVYKVYTIGDCYVVLGINDMEQRHPGQEAKNVLEMGLEMVEIIKRVRKEINFKDLNMRIGIHTGQFYGGIIGTDIVRYDIFGIDAVIANKMESQGEAGKVMVSEDTRRLINIHFPGEYIFTSNKEVDIPVAKKKVQAYFATKADILSRQDNSRYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SG12</accession>
    <name>A0A8S1SG12_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0060371</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SG12"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00106">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000083">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF15">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="10">
        <location>
            <begin position="12"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="748"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="379"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="642"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="115"/>
            <end position="122"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8137942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="775" mass="89114" checksum="73ACCAA86227EF8C" modified="2022-10-12" version="1">MKQKEKEGSNENLRVVIRVRPPMAREIKDGKFISTVQAAPDNQQLCIFDYHAIELVPDEELEAFVQNPANYTIHQFTFDYVYDQESTQVEVYETTAALSVDSTLQGYNSTIIAYGQTGTGKTYTMHGFSFTPNSDQLGIIPRSLHNIFTHIQMKSNSMTTFMVRASYLQIYNESISDLLRPDNQQLNIREDKKRGVFVENLSEWAVRSPPEIYQLMRRGNAKRVTASTRMNDTSSRSHAVFIITVEQIEETPEGKRARVGKLNLVDLAGSERVRVTGATGIRLEESKKINQSLSALGNVIAALTELKQPKSHIPYRDSKITRLLEDSLGGNCKTTFMAMISPAIEAFNESLSTLKFANRAKNIRNTPMVNQDQDQGALLRKYQLEIQKLKQELDERSKLPIDSMVSELEKERQKALEDKQEVMSAYEQRNRDLVQEREMRKQLEEKISALNSQMLVGGQKIEETPQFQNALEKQQKLIRQQYQEKLQELEKERQNIEEDKAQTDKYKQLLLKQRDIMIALTNRLNERDETILQLQEELDAYDKLQKELEDINQTKDSRIQQLVELLKQKDIEIPMNLELPANSFINNAKQQLLLAEMPSSKMILVADPSSNSYIQTLPQQGSYHHSSEAVTQYELKNQIDINKKLELDLKISRMEQERQKKELVSLQDQIQKYENDPTTEQAKKSVDLILNQLTKPANGNSLATVAQELQKLQKILSDKEASPKKAVLKFQQETSNLKQQEIINKLIIKPSKSNNQLNPPPNKKQKQKSIEDLWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SGS3</accession>
    <name>A0A8S1SGS3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0060170</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SGS3"/>
    <dbReference type="OrthoDB" id="411372at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039512">
        <property type="entry name" value="RCHY1_zinc-ribbon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008913">
        <property type="entry name" value="Znf_CHY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017921">
        <property type="entry name" value="Znf_CTCHY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21319:SF0">
        <property type="entry name" value="AND RING FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G08900)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21319">
        <property type="entry name" value="RING FINGER AND CHY ZINC FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05495">
        <property type="entry name" value="zf-CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14599">
        <property type="entry name" value="zinc_ribbon_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51266">
        <property type="entry name" value="ZF_CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51270">
        <property type="entry name" value="ZF_CTCHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHY-type" evidence="3">
        <location>
            <begin position="10"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="CTCHY-type" evidence="4">
        <location>
            <begin position="88"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="2">
        <location>
            <begin position="153"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8137594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="263" mass="30303" checksum="E44D316309BBCB56" modified="2022-10-12" version="1">MFLLNCLKKIITPIPNCPHYQRNCDKKAPCCGKFYPCRLCHDANYQGPNSNGCKTEIMDRYNVTTIRCRKCLCEQPPTNKCIQCGIQFAKYFCSICKLYDDDPNKDVYHCDQCKMCRRGVKEQNFHCNVCGICLSKSIQNSHKCLNQVVDNDCPICLQNLKVSTNYIMQLPNCAHFIHSKCFNQLIQSNQRNCPLCSIPIAKMTINEIEQYDKLAEDVKQLLPAQIQNQKVSIKCLDCRQISRDISSNYYLKCSHCGSYNTKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SHR8</accession>
    <name>A0A8S1SHR8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0060372</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SHR8"/>
    <dbReference type="OrthoDB" id="290533at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24362:SF309">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24362">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="21"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8137944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="368" mass="43857" checksum="70D29DB25D95F155" modified="2022-10-12" version="1">MGQEQSINEKTQEYKQFLLQYSQEREATHPQFGTIKLFTHIQTNEQICLKKYFTKSEKEFSNLVAHLRQRNSFEQTNLLKILAVHKQDQIKICSDQSQIAIIIQYYQENLTNEISIRKINNRPYKEGHIWILLQQIIDPCTYLCERNIFHGDIKPSTLYLDENGCIKLCECSMFKDGLNGYQKMIQSQDKSYLSPILLKQYKELIMLPSHDPYKSDIYSLGLTVLSVILMEEVYDCFDYVNGIVLTHSLEQKFIRIKQMGYSQILIEFIKQLLLFDEQERPSWQMLKEFIDKYRDKIYNMIPFYQNQLNNQKSPIRQSNQFNKSPLRQNMIYLPLNKSPQQQFQISQQFNTSRSDTKTKYVQISKENY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJL6</accession>
    <name>A0A8S1SJL6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0080104</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJL6"/>
    <dbReference type="OrthoDB" id="311264at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050907">
        <property type="entry name" value="SRSF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23147">
        <property type="entry name" value="SERINE/ARGININE RICH SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="17"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="5">
        <location>
            <begin position="97"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="106"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="115"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="128"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="140"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="190"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8139449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="219" mass="24481" checksum="4F7052B33B6F9970" modified="2022-10-12" version="1">MSSSSSSRSRSPRKNKKRVYVTGYSLKEDQHDIKKLFKKFGKIEEFAWKGKYCFIEFKDADDAEKAVKKMNKEEVKGSVLQVEMARGNKPSKNNGLCYSCGHSGHFAKNCRHRSSSSSSSSSSSESRKKSKKKHKKKKSSSSSDSSSSSSSRDKKKKLNKKRKSSKRKSKSSSRGSSKDSHNSESSGSVEGEKQESNNIKEDKQPQDEENQKKGGDGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SRI4</accession>
    <name>A0A8S1SRI4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0130040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SRI4"/>
    <dbReference type="OrthoDB" id="285309at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8144111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="153" mass="17338" checksum="5F33CEC38D6E2181" modified="2022-10-12" version="1">MDTKKLSKIIELIFLSIAWLSVLACLIRTDFNFPFAFFCYYLWISRDDKANSLMLMVLNGILILVDLIWLLSVGSIWTATEKNNPVWGRLHGLHVFVIFISIVNALLKVGAIVAINSYRGNQQQVAGPIGTQNPQQSGFNDMSQNRQQPYAQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SS47</accession>
    <name>A0A8S1SS47_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0110307</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SS47"/>
    <dbReference type="OrthoDB" id="304160at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="164"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8142728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="242" mass="28381" checksum="C67640E2E154D592" modified="2022-10-12" version="1">MTSIQQLSQAERPQEQQKNKSSTSSKDSESSDSSYDFVKDLNQKSSLKRTPEEEQELLSRYKGKHGLKYQKISNEQRQKLIKQVTTTGCTIKSAARELNINFSTAKAIMQIFRKEGRTSKKVKRDKTQEEFKNPEILIQVGLSKEEQCQPRQNTVQDNLDTMHYQQVEEKNRNQVIQIQQLNAQNLYLQGRINQLQQEKSLLCQNYSYLTLQYNQLQQMAQQINQNCQVRQAMPQYSKPYQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SS53</accession>
    <name>A0A8S1SS53_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0110323</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SS53"/>
    <dbReference type="OrthoDB" id="310602at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:001258">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045194">
        <property type="entry name" value="MGRN1/RNF157-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22996">
        <property type="entry name" value="MAHOGUNIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22996:SF0">
        <property type="entry name" value="RE60872P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="4">
        <location>
            <begin position="215"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="341"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="385"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="677"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="277"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="684"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8142760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="706" mass="83967" checksum="64B4C6C0260C7E20" modified="2022-10-12" version="1">MGNILKNQKEISKNLDQIIQSQVMDTPNILIKQSKSGNVDIIKLNLQIINAEIVYDSVDKIQLDLTFSSHVNFQILVYTFVTETKGAKQQRYEKSMPESTTQSFKCPSGLNYQFPSKYIEFMIIDLLRFKKMRVNPDAQYHTLIIEMKGLNSKGFQIIYFYRIDCNEQSYQCELINTKQIIIYKNRFFEIHELYGVQNTPFNPEWNPNTIEDKECVICFCNMINTVLLPCKHMCTCSTCADHILMSQKVKQCPLCRIEIDNYLTLEIKDKQKQDMQLRKFQEEQQKYLDSIKEKKEQQAIKQSSIMEQLKSKVQEEQLKQQQQKIKNMKYFNDVDDFNKDNQSNQDIFGNKSFCSYDENQSDPEYNQFNNSVKGKIQQQFDDENNFKFSNNYDEDRQKSTAHQRSPYDIGFLSSFQQQQEMKDSNQNHNQQNITIEDEICQNQTHLQQQQQQQQQISSQLLNKVDQYNLNSQQNSENQDQSSNQLLIQDFKQDEIQLQVNQDQRKESLEIIIEFQQNQIQPNQNQQSSNTQLINFLDESSEMNEEFKQENNRKFHSQQVLQQQPQQFQPKQQQQWSKNYKQSYQYSYDFVEQDLQDDFNQFQQQTRNFPKQNDFSFKTNQSQISNRSQEIQDINYYKSARQASDLQNEDQIFGNQNAQYFTQRLEQEQDIKKSASLIDLQDQEEEKKDPSHSKEFKSDQVSLIAFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1STK4</accession>
    <name>A0A8S1STK4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0100330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1STK4"/>
    <dbReference type="OrthoDB" id="296005at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8141832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="178" mass="20518" checksum="9B092BD60397DA0C" modified="2022-10-12" version="1">MGGMCQKQINIPEQENNIQKQPKIYIKGPPKLCKPSPIKILQLQTDDATIEKELCISIPKLLSQEKEFTFTEQEMPIKNVKNSLEEELLFRQESDQLEQQNNSKEEANSQKNEVKGSSKDMSKHKSILKNKIYNGSNPQSPKNQLKESQTFGSQRSIKKVTFDKKQKVVYSSFRKIKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SV42</accession>
    <name>A0A8S1SV42_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0110184</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SV42"/>
    <dbReference type="OrthoDB" id="310387at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8142484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="441" mass="53408" checksum="71C3D97605D090E4" modified="2022-10-12" version="1">MKGWQNQYEITQKVNRNDDSYLQKIMYFENNEYRNTQQIIDHIDYKFHQSQCFSLHQLIESMNYNFTKIYKGQLIIIILEVLKHLQQLHKQKISHRKLIPQNIIIQLKNDNKLTIIQTKFTIQKVHFINYSIIDDSNYQFNYIEDINQIIDCFIQFIQAFSNQQCQLFKTICNILNDYKNIINEDDLNKLIDFFNLLIQDCVDLQQQKLNKGQIYLQIKLNESNIQEIKYQEGEEYGILTKRQRNQNVILKFIEQLNVQSITYELQYHNNNNNENNYVDQIQLFDTSQLSLDEFKIFNVFESIIKQQMMKIIWYHIGAYLESWESDFCKLIEAILQEINQTIEHILKTELITILKEFNNINQLNKQIEKEIETDYKFQIKYDEQKAKDQFILELKIQSQNYSKQNFQSFIDQIKSNIKIELENYYNQFVMIEILQLINDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SV77</accession>
    <name>A0A8S1SV77_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0120032</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SV77"/>
    <dbReference type="OrthoDB" id="304018at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8143087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="639" mass="75330" checksum="F7764255FC019850" modified="2022-10-12" version="1">MKTKIVSLFLKREHLILLSKMLQITDNATDQDTQIQKIDSQNQKSENRILKTERWPQQDLDYFQNSFLQIANLTFSYFKFHFPANSALQIKEHIKFVHIVKQINFGGVTIEDVFKQQRTRLNLEVSKITKSIKLIDEPIMSKLMNYKIDLTYIQTIQDKYFYPKNNDFMAPYIILNLKPHISKTTTRYQYKELQQSITSLEDIPPFQFMNSNLIQPILNYDSLKNTFEYKFNKLMQRNQLVQYTIKRYSNKIKQYEIKSEDDHNDNSLKEIKKLFSIQIKQLSENKLNQNLIDSNLKLLNPQSFIDSSPKIIPLLFQSEIFNQIKEESQDINPFCYQQKYHNQDQDCRQTPKIMSLNISQQYLQDSANKYVETQKNLQISQNESYNKLESRLIKPSLRSLDFKQTEILNQSTGRYQKKMNNQKIQDQYDNSQSNNKFLNIPHQTIEPPITQIDVQTTDIQKTIQTNRNLQQKSLDRINLKPLAYQSARLKGIKDSNQKESSFPYIKESLPNICLSINDSHQNKLKESESKFSLQGSLQKKFQTNEKEKESLRDILLLQKKNSNLKNQLQNQLILLTHSEQILTTLMSNGSIRGTELYQQLLQPITINSSAKFLTPKSNNILSRAKFRKMQQESKMFSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SVU6</accession>
    <name>A0A8S1SVU6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0110350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SVU6"/>
    <dbReference type="OrthoDB" id="321909at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="138"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="175"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="259"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="314"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8142814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="369" mass="42861" checksum="860A53574D724F30" modified="2022-10-12" version="1">MQQISPEVEQYLSELLKPVLERVDELKIRDQQNEDQINSLVQELTQLKQLVSSLKPIKQEDEKKQIKQEQKKDNNGNHENKKQDRPTTAVVQKNEQNKVKEPRPQTGQKPLSQSVIVDKTTTNEIDKVPLKSPRDSTQKQPKPQGQQQQQPPQPKSKPQKQPEQKDTKDQKDSKPQGQEQPQKQAQPQKDEKKVEKPKQTKQNPPVQAEKTDKPKEVSKKADKPQDQKANGSQKNQTKQNDKKAEPKQEDKKIVKKTKQTTGQTGDQQQTDEQFQQQQQASQVSQEQQSAEKSLKENHSEQNEQQPDNHSEHTNNVQEVNQPPQEQHQEEQNQFQNQEQKEEQHQQPENQNQDDNQPKEESQSQQQEVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T518</accession>
    <name>A0A8S1T518_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0160011</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8146706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T518"/>
    <dbReference type="OrthoDB" id="118550at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8146706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="251" mass="29651" checksum="BB8B8C0B6113653C" modified="2022-10-12" version="1">MSQEEYQKQEEAPDANAQPTLGQVQQPIKRKNKKRAILNPQQSTGHWSEEEHKTYINFLNVHRSVMESSDQKKTSKIFKLMSETIGTRSPSQCRSHHQKFNPFSHQVKKRQKGAGRKKKESNSQIDKSNNGFIENACFPQFMFQPSAPYFPQQPFDFQMMQLQQVPFMMQYLDQANKDDEKLVQQDPSVYLQQFIMNPQQVYDEKNQFYNFGFPHPQQMQFNFNYFQQLPNELYGTNSAEIIQKDNQSEDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TB74</accession>
    <name>A0A8S1TB74_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0180403</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8149078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TB74"/>
    <dbReference type="OrthoDB" id="417811at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="373"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="6">
        <location>
            <begin position="527"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8149078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="824" mass="97525" checksum="AA1393356A21D949" modified="2022-10-12" version="1">MNQSSVIIEDNKYQSVQIEEERMLTAKACDFKKSLISKKFLQNYGKEELEDINQLFDERQLNILNDLANDNGHDSAQITTHREEINQRIQTAIENGFKRHSVMIIKKPVEIQRFKIQVFKPEDKFRILWDLFTMIVIFFAILILPLDISFTIESDFFDNFNIASITIFSLDILINFNTSYQQKGQYIYDRKLIAKNYLMAWFWIDLLSTFPFDLIINASAEEILHSDEIDEAQVQNVRDKQQQQQQIANTLKLLRILKFFRFIKVVRLLRVLKLKKIFSKLEDYVDLSNSMISLYKVIKLTFIMLFVAHWLACIWHFIADQENISGDYNWLTAQGIQDNDWYVKYIASVYWATATMTTVGYGDITPVTSSEKIFGIVVMLLACCIFAYIMNSIGGIFVSMDYNEKIIRQKMGQANQFLKSNDIPKDLQARVRKYLEYKYEKESTQVNEKEALEVLSQSLRVEVLAAVNTDLINNSKVFKQNKFEKELLLQLPFELEEQIFGPEECIFLEGDDPLEQENGQNLDERCLYFLNQGKVMLCIQKTFTCLKILEKGGTFGELGFFSNKSRSASVYTLDFVYVQKLKKKKFLELMKRFQTQNEYFQMNKHIIELNDDFTPLDLPCFGCQQIDHFAYKCPKLHFIIKYDRKQELIQEFQQAQKEVNKNYKRHDKIKYNSRGCYSLTNEVANEIKLVYNSEEKQEEDRLIQLQQHNEEEPTLAGFEFIEQSKQIQHQQEDEFERMSIMHAFFTQFNLDYIIKAYNERVDYILNQQQQQLNRIIRSNFKQFKSNKSKQRIRSMSAERAIYFEKLIEKYGGPSLFLQKLHEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TB94</accession>
    <name>A0A8S1TB94_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ABC transporter family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T0180414</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8149098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TB94"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18579">
        <property type="entry name" value="ABC_6TM_ABCC_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18580">
        <property type="entry name" value="ABC_6TM_ABCC_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000080">
        <property type="entry name" value="ABC transporter C family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000063">
        <property type="entry name" value="Multidrug resistance protein ABC transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000610">
        <property type="entry name" value="Multidrug resistance-associated ABC transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002673">
        <property type="entry name" value="Multispecific organic anion transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044746">
        <property type="entry name" value="ABCC_6TM_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044726">
        <property type="entry name" value="ABCC_6TM_D2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF456">
        <property type="entry name" value="MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN LETHAL(2)03659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="90"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="138"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="318"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="357"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="724"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="763"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="806"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="838"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="938"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="104"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="431"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="725"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="1026"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="658"/>
            <end position="685"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8149098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1267" mass="145024" checksum="87506E36CD424B00" modified="2022-10-12" version="1">MNNTEISQEEPLLENKSQRPPGLLSRTLFLYVFPLLRLANKTPLEFEMVKDLQIEDQSESLFNRMNSTFQIYKHERFALYKSLFFTFKSIFLIVNVEQFFIVYIIILIWNISLMYGPIMIRQTLSYIDNSEHTLGKSFQWLGIIIVVRVFNAITYQNSFYMLRKLGYDQHSAVSVSVMKKTLNVSFQSNKQYKTGEIMNIMQVDLQRILQLNIAVASVIFLPFQIGISFYLLFDFIGVSCLAGFGVMILGLLINFLLGRWGWKLQKQVMVAKDNRTKQAYEIFSQIKFIKANAFEDYFKNKLLRFREKEIQLIKKKNIVSGLFTLAFLMTPQLTLNVTLAVYIWLQYNLTPADTFTIISLFNILQQSASALPSFINQIIEANISIKRIQNFLLTDELMNDCIQNINDVLGNSIEISGTFFWDQIKNNQDPNKSTYIVPVNQVIDPILKNLQLKIEIGEFVTIIGDVASGKSSLISAILGEMVYNVQRQAPTIKINGRVAYVSQKSWIQNATLRDNILFGLPYDEKKYNDAIKYSCLEQDIKILDKGETTMIGEKGVNLSGGQKARISLARALYSDCDIYLLDDLISAVDMHVGKFIIEKCLCEHLNGKTIILITHALYSCQFADRIILMDNGQIIKEGTLDDIKECDKFDQIYQKYFKEQKNEQKDDQEEELEVLKLLKKKSSTQYINNTNKDQVDDLMILEDRKVGSVQSDVYKEYFKMNGGCLFFTFNLIVVITQVIARFGSQIWLAKWSGQNDLTYDENLHNLMIFSFFSLSFGFFALIRILTLSRESVNTANKIHTRMIDQLLYAPLCQFFERIPLGVLMNRLTKDQSVLDTEILWTISILYISCSNFIVLIFLYAVWKVQRFYMAANRELYRLESISKSPILSFFSETVNGLNIIRAFRKQEQFLDRHTKNIDLNRKIQIAQLQTTTWFSMNLTFTSFIVNISAIAFVLFFGSENPALAGLLMTVATVIDNSLQSAINSITQAETQFISFERCLAFAKIEHENGYKLKKDYIQNWPQVGDIKIDQLIVKYRENLSPALRGLNVVIKSQEKIGVVGRTGAGKSTVTLSLLRILEASSGSIIIDGVDISTLSLKQLRESITMILQESTLFEGTLRENLDPLHQHTDQELNDVALQCCLGELLLQKQGLDSEISENGDNLSAGEKQLISIARAVLKQSQIILIDEATANIDIDTESKIQQTIQTAFKKCTVITIAHRINTIMHCDKILVIDQGEAKEFDEPQNLLDNKSSIFYSLYLQGKKSNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TFT3</accession>
    <name>A0A8S1TFT3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0220276</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8152095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TFT3"/>
    <dbReference type="OrthoDB" id="292046at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ARM" evidence="1">
        <location>
            <begin position="345"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8152095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="564" mass="66431" checksum="F60E66E6AB43D87F" modified="2022-10-12" version="1">MNNNNIIPIQLLQQLRLPIRNKDYNTSLQSFNSFLGQVEQYIRLFNQQNLDQIKIIFGMILEDLDFLQLKPIIQIWRINFTELFLNTFNLSFAKALCQYYVSLSECSQIFKTTLFNEISKQICQIFCILCWDKDISDEIRHSKCLRSFIRMLRVNCTDDLLACLVIVTKNNKNSEYMTIKKSQDQLLQLFRNNINRELVQLIIANMCFNNDQRLLFWCQGILQVLKLTLDASSLTLIWKLLYDCPEVIDELLKYAEFFNHLYKQINTISEECALMIGIIRKICENNEQYKLQHIENIVNIFSNFLENNQIQSPKYDQQHPSIRQFIFKELFSFFGIVGNQYIQPQIIPHIISLIYQNSSDNRLLTVGIGMLTNLSTYPQILNIIQNDKLFYQIILQILEQFHDKQQLIEYTLKLMINTLTNQIISIQYAQPRFIMKLFFIWHVSPENISVNNLIIRVLRGLYVITNANAQILSQCINELQQRNNTFDFVDLCSLTINTNLQQNRLENVVEIYMLISIIPDLKLNSIKFQNTIKQYVETMRAKGELKKALQAVSHLPIEDIGLQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TGE4</accession>
    <name>A0A8S1TGE4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0220382</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8152305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TGE4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8152305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="49" mass="5949" checksum="F2684F1DF21DC590" modified="2022-10-12" version="1">MQIIQQIYQPRYEEFISDFDFQNLLILEQGAFAQLKKLRFKSKTLPLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1THI3</accession>
    <name>A0A8S1THI3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0240311</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10477991at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8153611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="146" mass="17474" checksum="81AB5D3C465DE6A2" modified="2022-10-12" version="1">MNLQKKLNEIQLVQEPTLCKGINQFNGISWDELESRFIYSTFQILLKDQEIQYIKEGSIIRQDIIKDNSKQPEYLANLEQIKFLKWIGSYNKNNQQIGKWIVTWKGETLQEIGGYYDEGKKQSLWKEXYEPYTLNQENYKIDQQDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TJ19</accession>
    <name>A0A8S1TJ19_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0220032</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8151644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TJ19"/>
    <dbReference type="OrthoDB" id="297125at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="330"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8151644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="367" mass="43316" checksum="01B1AFC3C448C6D1" modified="2022-10-12" version="1">MNQIKKLLVENLNYIFNILKPYKHQKISQFVEIQLQDFIQLFSRLQLIQTKLQQLFKMCDSLQKSGIALAQTQKQILKEKSEIQLQMQEIEKLLYDDTQTSLQETQKQVHDISELCGEAYKNLLDNQSFDLTKISHIQPPQMDDIGEWDSKLESSRDKTSEFDHIRKAFQVLDKAKLFEPSNLQMLMKCAENTDKQIYMDFFLQIINIQRKQQESNSKIVDERSPELREQILQMPICQEFSFESPLPSLREQEQPQQQFKHVKLEDLLKIEERLISDTSPNKQNFVKKQPANKSNIDNSFYSQHNIKHLIRRYPNNNILREKSPSEHISRSSSSLLVEQSQSTRQQKEVFKRKISQPTSITERNGNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TJ90</accession>
    <name>A0A8S1TJ90_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0210141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TJ90"/>
    <dbReference type="OrthoDB" id="290555at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="70"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8151039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="205" mass="24986" checksum="EE02F084EF8B476D" modified="2022-10-12" version="1">MFNKPRSNYLTLDFKKVKVKDEQISQPQTHRINTSRTILRKLRVDGCQTDRDRNSYQEVTNYTQEKLIYKQEFEDERKRKLEQLRCEVEELKLDYEQKQKELIQSRKQVNNALKGLVNSHTKYKDYQIGDVKLYQQYYRCKTETPFHLKNTIYDAQKYQNYYGKLQKANLKLNHQVQFAMIQYRKQLEESQQRIPNFLKSIQQVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TJM0</accession>
    <name>A0A8S1TJM0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0240122</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TJM0"/>
    <dbReference type="OrthoDB" id="285954at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8153275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="516" mass="61513" checksum="F5C6613CC9A9A0F1" modified="2022-10-12" version="1">MNRKFFQLRQMTRNLDDTFKEDLELYNKLLQKKYQPNPQRLNNDKQMLQQWDSFKQFRLSNIIDTTLDSDIFKICICDFLKKKRVSMEFWIKVAYDVFHDESKGKQVRMKFWRSMRKKSSSLELDLKNKKWPVAWRYLEREPCLNLEILKLYEQYMPIRPFFAQTLNTMTNITCDLRRMPMLKEEKSIEFDLSKTTWFPKIDMCGPSILEQLSSSDNYFCEQLVKYTTEMKSIMFPILDFFRLDKKKGRIINLFGEQHDFVLEVIYSLKAFCGQAINYTQGSLKKKKNYKCTNSYKINEMQCSIMLTTLSENDLNQDVMNDIQNNIEGGYSAFIQENQMYYHSEKNQRLVLIFSQKKLQFGRLKKGIKFYQIPDQQISTLKNKVDYEFLIYYANILFNKCMIVQQNCSSRKPFYQVMICMWTYVLAVYLSGEIPNLEGINVFQDNDRADSLVDIQIDLGLSSHEQTYEEPEFDQDQFQKTSFEFTNHVGGVSLDPSEEEDCMIVDQIQKKKKIILV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TJT2</accession>
    <name>A0A8S1TJT2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0220207</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8151957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TJT2"/>
    <dbReference type="OrthoDB" id="41200at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10971">
        <property type="entry name" value="MRNA EXPORT FACTOR AND BUB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="431"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8151957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="520" mass="60022" checksum="2214229562A15545" modified="2022-10-12" version="1">MSLDTLNGIEYLGAHLKFKVPGYREKADGQTYYRIVLQYTLVEPFKRSDLEYTYDNCLALCKTLTLQCPDLPQLPGIGMFGFKSNTLDAERRRVDLEKWLGALIWRCDVLGSESFYNFCELSQLINNKWLFQPKPSLKIHYGHAFPITDFHYEIDTQVLFVLTTDDSISSKMQGFMGIFGKSLKPSFHTQLICYRLDVNQDGQAVAQSLWTVQYQHRGTMIRWEEKLTILGIGFDDGHIVCLRIATNKQYLEYSEFCSIHKHTQSINGIAFNNLTGHCYSIGKDKCIITTDLANPSSILKEKEFTNELTCMTTNQYRLFVGDNIGSIYIFSFENGPLHAIHQIKQDITMHLTSIQYCPIKNYLICGSKEGYLSVFEIGKQGKEKNSNQITLLKTLPNKVVQFSISTKEIYVNYGNCVVILESTNMKYLYSLDAHDKDITKIQLFDSVGLLMTSSKDNSIKFWQYKFFPHILLNNTIIPYRKEPITELKVQIRQQGGEQNKQVQQVQQQMKPELEDLDHWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TNQ3</accession>
    <name>A0A8S1TNQ3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0240317</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="317926at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8153623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="413" mass="48234" checksum="E9A973CAFAFA8872" modified="2022-10-12" version="1">MSQFVDYEWKKECEGIIWDYIYNPPRKIRTKYLISRTNEKEIMYTLDGCSLKIDRIIGPSKELDLMNNLEQIKHLQWIGQYGQNNVKIGKWIIRWNGETLKDVGGQYSNDGKKQGRWIELFSNYWNKAQVYEEGDYVDDQRQGTWKYVQEDREIGGGQYNQLGEKDGKWIQSSSGYWDFSQVIDCGFYKNGKKIGKWDILYRKKGKEQLQYRGGGQYDEQGSKKIGKWIELSDGFYDLSQISYKGEYQNGKKVGKWQILFGENQMQHYLLIIFFSSGGGSYAEVDQIKIGKWIEQSDGFYIDSQIIYDGEYKNGMKIGMWNIYLKDKKSGQTQQIGGGQYDQNGSIKIGKWIEQSDGFYNLSQVIQKGEYKNGKKVGRWDILFREDFSEEFLLIGGGWYDEXILQIASASELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TPZ7</accession>
    <name>A0A8S1TPZ7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0240314</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TPZ7"/>
    <dbReference type="OrthoDB" id="5337378at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="17"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8153617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="518" mass="61709" checksum="FC944995D45ABB1B" modified="2022-10-12" version="1">MDQINDAAQLFRKKYTIIMDEIYGEGCFGQIYSCKKIDDSDDNIYCAKVIPINVDKERSKENEIIINNSIAKLKLNQHQQMNLVKIYDISFDSNSLYIIMERCDEDLSFEFYKLIEENSWYTEEEVYDLIQQIINGIKEMHSKKIIHQNIKPENILIKYENKGEINERKIYKITDYEIGILISKLTKKQDLIRTGSPNYTAPQVFEDDVIYSEQSDIFSFGVLFYQICYQAKFPYDCSSILQYLKNNPFKAPPFNYNNGKQLQNLIENMIVYNENQRISYSTLFTHAVVKRKSQEDTVIIRNESRNFRSQRLFDKSINFENKKKTQILLTLERLKLLLEQFLIKFQICQQLIQQIQDSDEFLFFKLNIYLIGNYQLFYALALIYIRPQKLHPSILKYNDQLMLIEKLSEAQEGIKIYHPNTKIYQDFYDSIQSQYHKFVYQFKEFYINIKRRKELPNELEEFTQQAVSRRIELTILLQNIKNLKKTYLSIKFESLSPISLNQKNVDQQSQPSQQIGQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TVU0</accession>
    <name>A0A8S1TVU0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0290216</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TVU0"/>
    <dbReference type="OrthoDB" id="433501at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044640">
        <property type="entry name" value="RU2A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552:SF6">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8156865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="218" mass="25310" checksum="2FF491959DA25E7D" modified="2022-10-12" version="1">MKINSELIQGSHHFINPLNERQLDLRAKKITAIENLGATLDFFDHIDLGDNDIKKLGNLTLLKRLKTLNLSNNRIIKLSDISDSLPNIENLILMNNRLTDINEMYQLRNCKKLKRLILHGNLITQQPDYRYKVIAILPNLKVLDFNKVTLAEREKAVESFKPDELTDYMNLVNLKDATIDKDHIKKLLENAKTFDQINQLEMLLKQQQVKNLSILNDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TX14</accession>
    <name>A0A8S1TX14_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0290120</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TX14"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8156690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="214" mass="26154" checksum="A61E4A4CABCA036B" modified="2022-10-12" version="1">MSFQFSNIKNIIKSLCYYEAIDIIKMLRKLINTEFEQEYQDTKIKGIIISSSNFRIIMNVVQKIEKIQQDYQAAIVLEAQYRNYSVNIYCHWKLKKMLQIQQLKTFRHLIKQEINYQINNPYFDEVFKDFLNNFSFQQYREYFEQELIKLQFKFGLKLYDLKLTNNIEYPEFLIMNISQEFIRNVTQSDDSFKMKMMIILSTLLDYFKIQKMKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U0X1</accession>
    <name>A0A8S1U0X1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">phosphoinositide phospholipase C</fullName>
            <ecNumber evidence="1">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0300252</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000030">
        <property type="protein sequence ID" value="CAD8157603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U0X1"/>
    <dbReference type="OrthoDB" id="269822at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00275">
        <property type="entry name" value="C2_PLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08558">
        <property type="entry name" value="PI-PLCc_eukaryota"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF36">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE PHOSPHODIESTERASE BETA-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00387">
        <property type="entry name" value="PI-PLC-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00149">
        <property type="entry name" value="PLCYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="133"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="PI-PLC Y-box" evidence="7">
        <location>
            <begin position="523"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="6">
        <location>
            <begin position="623"/>
            <end position="752"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8157603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="770" mass="90712" checksum="C3753BF9ECEB879F" modified="2022-10-12" version="1">MNYSTVLRKKSFSGQKHNQAINLLVYGCEIQKIASGNKFEKLFIYLSHEISYHLVYIINQYTNYIDLRKIRFITDDIRDTKKFTDDKSVLLQIFVNEECWYLKFANQTLKELWWQGLQYFYTKAKEDLKEKLQIQLMAWEIFTKFDEDRDGFLNKEEQQVFLNWIKFVYPDKNLIETKCIQSSFQNKLEYKQFEEIIKLLYEQSILINLNKMYLNSENLLTEQDFKKFINTAQRKQQTDGFFGFPMTADKFYDFLFSQQNSIFEIGNSDEMTKPLSHYYINSSHNTYLTKDQLSGDSSVGQYVIALLNGCRCFELDCWDSYFDKNPVIYHGFTLTSKILFEDVIMALEKFGFMFSSYPIILSLELHCNIDQQEVLGQIMRTQFKDELYIYEQGLTVLPSPEKLKRKFLIKSSGILEESYNLHQNNQVIRKNMMNLENTKLQNTIQLIQIDQDPEPGLIKEIAQKQRINNKALKNLNYENKQKQYMRHDAICDFKMTGDQVDMVIHCSMNVKHHPQFLSCISLFNSPFEFNKKRTIWNISSLSEDKVDQIFKGGKVKEIQNHLNNSLVRIYPSGLRMNSSNFDPMPSFILGAQIIALNFQTKDEPMLINQARFQQNLGCGYVLKPKYLIEGLDDINNFEFQKKLITIQIISCQLFLLNTKNTVNSFVTVKVKGNKMDENGQELQTNIQYNNGLNPQFDSNYSKINFEIKDVDQAIFIFKVFNKQDDNKCTIIGQYAVPYKNMRQGYRAIPLKDENLLVMEYCYIFAQVTIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U2N3</accession>
    <name>A0A8S1U2N3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Myb-like DNA-binding domain containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0310255</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U2N3"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF271">
        <property type="entry name" value="MYB DNA BINDING PROTEIN_ TRANSCRIPTION FACTOR-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="123"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="2">
        <location>
            <begin position="130"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="186"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8158272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="340" mass="40167" checksum="238B844E82DB041B" modified="2022-10-12" version="1">MSFLHVHQQSYHQSTEESQHSHKLNFLQIQNVDIKEYKRKWMLHFNSIPNYKVLEGMYYQLQQAKTSDDRMKNEEILLQCILISVQAPLSIKLSDEQWLLVSELMPTYRTPKELQEMSLQFLHQSAYNNPWTDQEDQQLLEIILSFLKQKKGNKWSKIAKELNDVSQSKFMRTPKQCRERWGNKLDPSINREEWSDQEDLYFLQLLLQHGRRWAEIAIRLSAITESKKRTEFALKHRYKKMILCSNTSCSKLIIGHKSSVSSDWNTKEANKIIAKISQLEKVIEPEKSNLYNCQPFNKQLKLNDSSRLVLIRGSCKEEIDLSKLFHLINLMNLPLLQGQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U624</accession>
    <name>A0A8S1U624_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cysteine protease</fullName>
            <ecNumber evidence="4">3.4.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0340151</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase C54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.-" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8160098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U624"/>
    <dbReference type="OrthoDB" id="433762at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019786">
        <property type="term" value="F:protein-phosphatidylethanolamide deconjugating activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035973">
        <property type="term" value="P:aggrephagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005078">
        <property type="entry name" value="Peptidase_C54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046792">
        <property type="entry name" value="Peptidase_C54_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624:SF49">
        <property type="entry name" value="CYSTEINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624">
        <property type="entry name" value="CYSTEINE PROTEASE ATG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03416">
        <property type="entry name" value="Peptidase_C54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase C54 catalytic" evidence="5">
        <location>
            <begin position="57"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8160098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="406" mass="48495" checksum="B29DE2D3F057F4F4" modified="2022-10-12" version="1">MDQRQENYKEVIIDWIYNLKYILNAYLNNKNVPSGHPIYILGHRIDIDNNDIDDRINKIKQLVQDTIWITYRRNFPPLYQSNYISDTGWGCMLRVGQMAMAQMLKKHLKNHGDKRDEDYDNILLAFADNDSQEYKEFIEFSNKNDKSKVHNFICPFSIQKIAYLAKKEFNLDPGEWYKPNYILFLLEELHNTIPIRASENLKLCVFNDSCLFLDQLMNRMFEIKFETDKDLEEQLEKTQFKSKNSLAIFVLTRIGLDEPNQKYLKVLDELMELPYFQGIVGGTPKRAFYILGRINDHYIYLDPHYVQEAENKDQIIENKMFNRTSYSCKYIHLLNQKHVDTSMGLSFYIRNKSELLQFWRDMKQIKQKSEDFFVFLSDFTPEYVDYSNQLEESNNKLNDDDVVFLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U6F7</accession>
    <name>A0A8S1U6F7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0320229</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8158929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U6F7"/>
    <dbReference type="OrthoDB" id="308936at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="200"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8158929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="824" mass="98797" checksum="6AE1E8643F57E4AC" modified="2022-10-12" version="1">MFQINQSILYFWFNVNANSELFHQITIEVVNLFQKIQINLYYVIKSLSPTYHYPIMQQKKQKKLSKSPQRNPLTTNTSLICKTTNISPNKIVKKRGTSATIPTDSYHIEKRDQVEKRKVSKIYKLTNQEKNNFVRKESKLKEIQRRQEIGLDFLNKIPKKKIQLLLQRQKTQGLSNFQQDNSKKMQSTQNQRLHLKQKNQLHQQQKQNEQMNRKKDMKKQLQNSRNILKKSLSQQKCKKRSITSITQKKEFTQRNSSSKTKNENTTQLTGYEGELLEKYKKLQARYSKIYSAFFDESSCVQYLTDTDPYLLPSQPTSNKQQLILTEEFSLNRDEKIQLKEFGILENKFNRMHTAATKIQKVWRGFMTRKLILDQQFEQYYYKQPEIFNEETFRSQFRFEKDSQSIQQGREQTLNQLTHQTDINFILELESGKVDKEEHKSSPKISGKFVKYQKMKWQELLNYISKLENQTQEKTINEVLKDLKLFTQQCSQDCSLSKFQIKKLEIQVPQFSQESMVMSEIRQAMCSEKLMRQILTSSIKLENESIFEEQPFHQFASKQFDEILYRNQMDELIKMRQLIVQEQQKSQHQALTQEFEQKLISPKTFEERYQTLEKWVTKQQEDIENNKINFEKGWQGLYNTFIQTEKELKFLQQQKYSQYSQISYSQQFINYSISAFSDSVYKNSIQELSNQNETLLLNNYNEEFENKRLKTHTSTSQTPDEQIKYQHLENYHETIEWNDEQLLTSQQFQQNHYVQDSKINNALTNAPLKSLEQAEIDYLHQEVQNEELNWLNYNEFKFYVLMDISNLIFNELIEEFKEECIQLQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U8Y5</accession>
    <name>A0A8S1U8Y5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">E2F/DP family winged-helix DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0360275</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the E2F/DP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U8Y5"/>
    <dbReference type="OrthoDB" id="10329226at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000360">
        <property type="entry name" value="Transcription factor Dp-1, a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003316">
        <property type="entry name" value="E2F_WHTH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015648">
        <property type="entry name" value="Transcrpt_fac_DP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12548">
        <property type="entry name" value="TRANSCRIPTION FACTOR DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12548:SF9">
        <property type="entry name" value="TRANSCRIPTION FACTOR DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02319">
        <property type="entry name" value="WHD_E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01372">
        <property type="entry name" value="E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="E2F/DP family winged-helix DNA-binding" evidence="2">
        <location>
            <begin position="154"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8161615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="364" mass="42991" checksum="B08AD265F3737B04" modified="2022-10-12" version="1">MCDQTPIEYRSEFESSPMNFGQPNMILFQPYSPLQLRINCSPNNLLIQDYYSAQPNQVQEDLFPFDQQPLQKMFELQISNDESPQIKQIVIKQSNTKQSGFQIVHVNTQQMTPQKYNNQHMNRHHALQKNISYSEKIEKKNYNYYQYQEQTPLRSQKGLRNLSVKVRDIVMELKSTTYKDVAQRLIQDLGQDGQFLDLDNPKDEQNIKRRVYDALNVMIASKVLKKEGKKVISDMQGKTRTRRNETDNQKEELQTIKSIIKEKKKRLKELFTKTLALENLIKRNSTNELKNKFMFPILTFQGHQQQIKLRMDSKVLKIMSQQRLALNADLDILVQMKMQQIYKNDDLFIPKELLDIVQVNEFCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U9X6</accession>
    <name>A0A8S1U9X6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0350005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U9X6"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8160479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="72" mass="8728" checksum="C182856AEBE55B8A" modified="2022-10-12" version="1">MMILNINNNQTKNYLKKSFLEKQLSEINQILIIYQLKIRLLQELENRDNRFRIQIQTRQVNLGISQEIIINQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UA40</accession>
    <name>A0A8S1UA40_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nuclear nucleic acid-binding protein C1D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0360048</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in the recruitment of the exosome to pre-rRNA to mediate the 3'-5' end processing of the 5.8S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer and homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the C1D family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UA40"/>
    <dbReference type="OrthoDB" id="10261072at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000178">
        <property type="term" value="C:exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011082">
        <property type="entry name" value="Exosome-assoc_fac/DNA_repair"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15341:SF3">
        <property type="entry name" value="NUCLEAR NUCLEIC ACID-BINDING PROTEIN C1D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15341">
        <property type="entry name" value="SUN-COR STEROID HORMONE RECEPTOR CO-REPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0698">rRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU368003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8161194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="166" mass="19637" checksum="E956142F2C35030F" modified="2022-10-12" version="1">MSISTTLQSTQEELTKLQILIQKLSQKGNLDQITEAMTYLKLNFVETSAHPIMNELLRIQEAYQKYLPSQIKQSDQKQMSLDREAAKRFIQPNLKCLDDNQNYQQKQKKLIEEDKNNTTRLEQPVDIWKNEKDQQDQNKQKLIRGNLIPQTGHLKWKDQIEKLIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UC04</accession>
    <name>A0A8S1UC04_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0380001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UC04"/>
    <dbReference type="OrthoDB" id="21182at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8162370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="258" mass="30452" checksum="120846976CB2B08C" modified="2022-10-12" version="1">MHLLTQQNYQIIQFQFNNYYQTQKNQNGLKNVLSSYVIQLFVIKNSEISKQCVFGNTRSENECKSSGFCIWENDFCILNSSRLYNIEINSQNLCKNFQQEDCREQEHCGFYFGQCQDFIYCNILDKDNCQESSYKCVSDGSKWVQMLECSDYKIENGRADKNQNGNYCFRFVVTKKKCKNVNLCEELPIYLTNHSIPNEGLNGRTINEQGYGCIEQMESCSQYANYFLCLKRKIIFFWMEQKHNVLKMLRKSTIYLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UGI7</accession>
    <name>A0A8S1UGI7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0390234</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UGI7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8163464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="82" mass="9631" checksum="AFEEA87903441BBC" modified="2022-10-12" version="1">MQSTPLKKKKSIIQKIVKFFNDIPDQQIAVINEASQKLPPQIKKSQSLEEKSQKNKKLDLKHKYSEELFEELHEVNRLELGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ULZ7</accession>
    <name>A0A8S1ULZ7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0440058</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ULZ7"/>
    <dbReference type="OrthoDB" id="315997at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8166268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="452" mass="54081" checksum="3F2EE0FB0D6D6432" modified="2022-10-12" version="1">MMKIVKSMAFWNGKVLKDVGGYFLDGQKQGFWREIIHNYWSQAEVYVIGEYYKNNKVGVWKYIFHNNIIGLGQYNYQGQRIAKWIELSDRFSNRSQITYIGEYQNGQKIGLWEIWYKDYETKRNKQIGGGLYDKINSIKIGRWIENCNNFRDCSQVIYQGQYKNGKKVGRWDILYRNQQSEAFQQMQNYIIKIKTKFSGGGSYDDQVQGDQIKIGSWIEISEEFYKWSQVTYQGEYKDGKRIGKWEIWFKDCHKNQKKLIGGGFYDEKSSTKIGKWIEQNLRFYYNSQVTWTGQYKNGQKVGRWDIVHEEQQMQIYIQISKYSGGGLYKEDGDSEIKIGNWIELSHNYRDWSQVTYSGEYKEDKKVGRWDIWYKDWETKKNEQLGSGHYDEISSIKNGVWIELSDIFDRYSQVIYDGEYQNGKKVGIWMELEKKRWKLQEGFKKIKDINYDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UNW0</accession>
    <name>A0A8S1UNW0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4378 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0420210</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UNW0"/>
    <dbReference type="OrthoDB" id="303744at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025486">
        <property type="entry name" value="DUF4378"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14309">
        <property type="entry name" value="DUF4378"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4378" evidence="3">
        <location>
            <begin position="829"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="451"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="552"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="188"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8165432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1030" mass="122841" checksum="3EC2540B4270A00C" modified="2022-10-12" version="1">MSGINININLNLDKNKVAKSKVDKICNGEPKKKKKKKKLNKSKQTTYSPIPWGLNQERLKKYIYAQMVKRDNLLYQEQLRKVKFEEPKQDRREILGVSFLNHLPKRKIKQMINKQKINEDEEQMYYQPKFNEQQIQEIRMYQEWKRKQIQFELQFKKQQELQRQSKIQQNLQNLNEYIKYRNHTTSVHQKKSVSFHQKKRPKSSMDDKKRELVIKYQNIADRYAQFNRDTRDNQSCLGLVLDSDQEGISQMDSIHKSTRRQNVIISEPSLDEDDEQQIQELGNLEKEMAQIIKAAIIIQKFWRGYRTRNELQYYQNFLDEDEMKLNQLTPNLNQTQSYLQPISQLEKEQSGLNSSQKRINKNGFNTFIHKLDWDEDKESQFSQKKVSSIKKLSQQKSHHKIIIQKSKQQWHLMLEQISQLQRKGSKQDIKEVLQDLKTFTLQIAKEIDGEINIDNKEKQQQQQQQQQQQQQQQQQQQQQQQQYQQPQQQFKINEIRQIMGSENLFKLSQISSPANKDASLFEQNPFYDFASNKFRELLNPENMNKLIKMREIAIEERHKNEIENIHQALQQKQISLKTFEKQKFKIEKWVTKEKEDFKQQKSNIEIGWKGLYESFIRTQKDLLFMSKLKNQQLTSSFSSENITNIRSIREINSASQSKIVKTNNFDTVTMNFSSSICESQDIRKVPKSTPSPSPHQQQKQIYEQLEWDDEMLYTSDVLDCAKIQQPQVIKVSTLIQKEPEYDDSQIQSYCILIANSIIGNEIQDMYEELKIYNKDITKSIKLQELKGIKSNISQVKIYLTRLEQYFLQSDKKTQLIQRINTPLGPTPKEILKFFHYYDEEDDYQSSDQDYSNQAILTTEQYAQLENQLMEEELMQSDDGFLLELEHIHNKVLFDALNEALDDYRPFGLNGFPLPWIKQPLELIQRNKKQETLQQILQGAIQQVIDWAQFLVGLIIDKPDSPFPKILMLDQEYLNQIKEERMIRMLNQEIYQNEERWLIYDEEQSEILVEISLMVQDQLIDECLKDLLQFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UUS8</accession>
    <name>A0A8S1UUS8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0490025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UUS8"/>
    <dbReference type="OrthoDB" id="299033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035895420" evidence="1">
        <location>
            <begin position="18"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8168315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="297" mass="32606" checksum="41C52702CBB902A0" modified="2022-10-12" version="1">MINKLIVSLCLIGLTTAVTYDSSLYCDCSELDQTDCASALGYCLWNSSDSECQDFDLDCGDITASGSCDVFDSCQWINGSCEDVEDLECSDGSTSNICRKIDECYWNKESKCSSFSKCDDYNVEDCPYGQGCTITSGSCAAVQYVTCSSFTTALTCDGQDSKTSRCAWGNDNTCKSLNTDYSCSDLNNFQNICNNSGTCIYESNTCRERKCSDITNSFACDRIRSADYKFTLCSWTNGACAVADASTLTQNECYQKTSGNYKWADNKCVQCDELNDKFSESNSYILGAVALLFILLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UUY0</accession>
    <name>A0A8S1UUY0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0480164</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000048">
        <property type="protein sequence ID" value="CAD8168053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UUY0"/>
    <dbReference type="OrthoDB" id="408117at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051480">
        <property type="term" value="P:regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051001">
        <property type="entry name" value="Calbindin_Ca-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19972">
        <property type="entry name" value="CALBINDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19972:SF10">
        <property type="entry name" value="CALBINDIN-32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="5"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="46"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8168053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="85" mass="9963" checksum="47243E2E57307C56" modified="2022-10-12" version="1">MNSIQVTEFSNRVFDRYDKNHSGFIEKDELNTLLKNLAMELNSQQPTQKEIDYMLSYLDTNNDSKVSRTEFQRLGELMVKVLGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UYV5</accession>
    <name>A0A8S1UYV5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0520032</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000052">
        <property type="protein sequence ID" value="CAD8169891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UYV5"/>
    <dbReference type="OrthoDB" id="2013972at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="119"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="164"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8169891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="382" mass="45107" checksum="802BF9D4089C804E" modified="2022-10-12" version="1">MQDQLKQQQEYNQEIKQSISQCEQLNPQFEDQLKVKDQQLLQQQHQAQTKIQQQFLQSSPELIQNSFTYQFIEESSIRKQDNCQAIAINSDCSIVIVGCYEKINVFQLKQDVLKEIQVLNEHQSIVNTLQFMKKSKQFMSGSYDKQIIIWSLNENNKWVCQQKLNGHNGWILCLLLNINEDLIITGSEDNTIKFWMKQNHYWLCSQTVTDHNNYVVGLSLNQKQNQLISCACDEQILIIEQSQQDCKWSVVQKIKVETTGCRICFINENSFIFQLYCVEYLHFYEFNDINKQYTKTKDIAMKSGGDYQFFPIKFIEPQCLIVIKTSNYVNFIRKIGNYEFKIEQSIQFGNFDLFGCVSDDGQFLITWDEESKEIQIRKYNQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V5E5</accession>
    <name>A0A8S1V5E5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 6">Inositol-pentakisphosphate 2-kinase</fullName>
            <ecNumber evidence="1 6">2.7.1.158</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0540071</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Phosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>1D-myo-inositol 1,3,4,5,6-pentakisphosphate + ATP = 1D-myo-inositol hexakisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20313"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57733"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.158"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="6">The EXKPK motif is conserved in inositol-pentakisphosphate 2-kinases of both family 1 and 2.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.158" evidence="1 6"/>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8171019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V5E5"/>
    <dbReference type="OrthoDB" id="294329at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035299">
        <property type="term" value="F:inositol-1,3,4,5,6-pentakisphosphate 2-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009286">
        <property type="entry name" value="Ins_P5_2-kin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14456">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14456:SF2">
        <property type="entry name" value="INOSITOL-PENTAKISPHOSPHATE 2-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06090">
        <property type="entry name" value="Ins_P5_2-kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8171019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="342" mass="40160" checksum="61D829673A8DF308" modified="2022-10-12" version="1">MDQYTVLGEGAQHIVYQYIGNDPTLKDKVIRIRKPRDICEISSNLILPFNNTDWTPLKKYFAKKQPYQIGDQQCQIMDNLFFNVVFAVEIKPKTFLPILSNKQENKDLCDPKNQRFFMIQLLKAQKKLQKNGKDIYQCTKMEIDSLISKYDPRKFYNGKIENLFDAIIDLADIPENNFRLFNNQQKHVQNIEEFNIEEIASIISETLLLEDLIQQLKGFFHMLQQMNLTVEETQEAYEKLKQKKLTNKQFQDIVEGKCQDPEIQKIAFKLLTFSSFQALSDLSIIGSFRKEGSGKFVEVGNQKLFYQYSIVDCDLKPLNKIEDYMSTIDELIKLRNYYDSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V8C0</accession>
    <name>A0A8S1V8C0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0580203</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000058">
        <property type="protein sequence ID" value="CAD8173077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V8C0"/>
    <dbReference type="OrthoDB" id="21204at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051834">
        <property type="entry name" value="RING_finger_E3_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931:SF3">
        <property type="entry name" value="RING ZINC FINGER-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931">
        <property type="entry name" value="SI:CH211-59O9.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="263"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8173077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="318" mass="38086" checksum="FEE4CA3C94034745" modified="2022-10-12" version="1">MLINRRYSKLFYQGLQHTQYVMLMCRAYSIFKFMMTLIFTLTINCERCELTNWMLVVLVHSIIAFVYHTYMGVLLNNIQIVMQIAESLNHMIQIEEDQDQVNELSESQYVINEELDPYSYLSQEEMEKKQKVLAVQIRCEVALRYKVLRLLGIITFWSTQILVIWALRLQLLNPEDPELYHACFRHVITFQLVFLFLTMYQYLEVYMVTLLIVICLPFLIPVMLWHKFKQKKQNYDNQQSLNQLKKTCKMLYHSEKIQGDQECGICMHVYVTDEELLILPCDPKHHFHLHCIQAWLLINSTCPKCRASFLRFKQQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VHB7</accession>
    <name>A0A8S1VHB7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0600249</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000060">
        <property type="protein sequence ID" value="CAD8174146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VHB7"/>
    <dbReference type="OrthoDB" id="301139at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8174146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="285" mass="32952" checksum="44D022DD7351D417" modified="2022-10-12" version="1">MFLKGKKQSNQDNFNPGVGLYDIITKLDKPVPIMHPDKHSLHKKNDSYPQSDSQLSFDRKVDLNYPLHSYASGIQRVRQNKIYQELKKQVRFDNKLNEFNQEFDTQIRSTNKKGKLVKLQKLQRCSKQQLNDSQSLCLGQQSPINKKSSSQQYLKHPPKLQSLESLELFLLRDKLQKIQDVPGPGSYELPSVFKQSDSRVQPFGKQKGRTKYIDQDVSVGVGQYNIQDQSIIKSVIRFDKYSKRVSIFENKQNSPTYYDNNSNLISNQSKLNLEFPIAFGSTSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VLC3</accession>
    <name>A0A8S1VLC3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0690037</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VLC3"/>
    <dbReference type="OrthoDB" id="319390at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8178194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="591" mass="71673" checksum="FA83840D6C656D3B" modified="2022-10-12" version="1">MKDNKENSAFKKENYHRHTFQYQLEQTNKDFEQIILKKQNIHSDEDYQALKAQWKEEIANNHKFDPILIVQDNSQSFIENSQILIEQGKWDDAIQLCDQGIKKYQNNMNLYQNKATALRGKGLLKQAIDIWDEGILKNDWNMEFYNEKAKELRRQSNLIEVLEFWNQQIQKEPNNIQFYQKKADCLKEQESFEQIFELWEEGIKYNNNNINFYIEKAKVLEQYSRFSECLDMWDQSIKIKINDINFYQQKSKTLVRIGKLQEAIDFWDQGIEQNKNNHLFYQAKERELKLQGKYDEIIQFWDQMILQNPSDIYYYEQQGIYIQLIVQLKVQQNKKNMKIIQLGKVLKHEGKINEVLECWELGILNNDQNLYFYQEKGNQIQELFQANALKDLNKFQEIFNFWQTLIKIDPQNRSYYEELTKMLNEVGQMGQIIDVWKQGIQNNINDIYFYEKTSQTLIEQERYQEVIECWTIGVENNKNNSIFYQEKAKALYNQGQINEVIECWNEGIQNNKHNQYYLDQKIDFLKNQHKFQELIMFLDQLINENKYQIKWYEEKCEALTHLQQFNKALRFQRIIIPKLKNFNYYCIKNDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VNH7</accession>
    <name>A0A8S1VNH7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0670052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000067">
        <property type="protein sequence ID" value="CAD8177159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VNH7"/>
    <dbReference type="OrthoDB" id="305386at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="381"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8177159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="528" mass="62595" checksum="81E3E575C02F94BB" modified="2022-10-12" version="1">MFKCIISAFKEMRMRSQIFLFEIIILLFVLVLAGAATYVELRIFYYISTHSSETMISKLDIKQINQATTLVSHYIKQKHQRRINLIEHLSSFLITYQIQSPKFTKVKQMESCINSELLIIPQYFYQTPKFCYQLHGVHDLITLPQNNQNISLLYDSLGLMNEYDLLFSIESSYFIQVVDNSDYKFDIVYPIGMLINGYDPKTRPWYVNHIKQMEVHSNELYFYSEVYKVFIEQFDYQFSITHSLFNSKKQFIGIAKTMLTTNDPSFEYVLYNIILINYAGQVLYNGMEMWQNLTDIFYVFNETITGFNQTDWYKIEQHAINRLSRDNSNQILILYNKYHKKFVHVQCEKFQKENFTLIIFTNVSSINIMEKQFQEIEQSFIFWYFLAASIIISICSLTITISLIVINSICQPLIKLTNQVSLHVLQLGNNINQQIFKMFNKQIYSLNLLNNLNNKFMNFQELLLQSQTTKCETCRLLEKMNFNKKESNINLKLIQNQNILLPEFRFVEQIQVQDIIKKSLFQGESMII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VRS9</accession>
    <name>A0A8S1VRS9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0720127</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000072">
        <property type="protein sequence ID" value="CAD8179607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VRS9"/>
    <dbReference type="OrthoDB" id="288050at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032727">
        <property type="entry name" value="CLAMP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28457:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 119"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28457">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14769">
        <property type="entry name" value="CLAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8179607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="294" mass="35357" checksum="3730EAEE1A2257D3" modified="2022-10-12" version="1">MSSPDTNSNRHQTKKRTKLDSNKSYSFYDSFVTRKDIIKLASLETQNQQREFLKTVIMRFEIGHKKGGVFTFPVYELISFQYYQTQTISKATEESQQQCKLAVFSEYLLQMHNYLKNYTFEQKSAFMSLGFYIFVESLERKITQIDSYEILKKLLRPKILLTNTYNLPIFTQEHIIEFNKFMTSYFYRYYSLYELRMTTYQEVNIFGRLKGENPVQIEKEEDKDSIQEEDEFNFKDVEVHLDDEVQPEQEIKKINEKDEEQIEKLLEQYKLNWNTRLDGSQLLQEAEKILTKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VVI5</accession>
    <name>A0A8S1VVI5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0760089</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000076">
        <property type="protein sequence ID" value="CAD8181360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VVI5"/>
    <dbReference type="OrthoDB" id="10266058at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12232">
        <property type="entry name" value="RRM3_U2AF65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001286">
        <property type="entry name" value="U2 snRNP auxiliary factor large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23139">
        <property type="entry name" value="RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="197"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="33"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8181360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="429" mass="50050" checksum="4BBC861D1BE03E0F" modified="2022-10-12" version="1">MNLEEQPKESIERESDDNEKNDKKDQVQQPEQKKKKKKNKKKKKWGEEEEILTDFDDDWQNKVKQSKILQDPDLPQHIKEKYAIYENNPFMMIISNVPLNVQLKELEEYFNTLITSLDPKINDRPIKSIEYGATKSWVVLECSSKEAKRALVTQDQVQFVNNCKIKVERPRKFLERILNPQARDGELNPEQKQEDNTRLYLGGLPTYLRDEDVMKLIQSFGITKYFNLVKDTTSNTEISKGYCFFEYENSVSTAKALKALNNLQIGDKKLKICKVQGETQQNKKVNGKDQPSNYAGSFLASCDLLRIPQVQQMLTIPQSALIPSKVVQFLNMCSVQDLYEDDIFEELMEDIRTECMRYGQIEKIEIPRPDKESGFCNPAVGKIFVKFYYQIPAKKAKFHLAGRTYNKRTVITSFYPEEQFDYKDYLING</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VW16</accession>
    <name>A0A8S1VW16_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0690231</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VW16"/>
    <dbReference type="OrthoDB" id="74764at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="131"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="440"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="464"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="478"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8178506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="531" mass="61393" checksum="631C78F7767D2049" modified="2022-10-12" version="1">MKQKLWIAIPNELKWKNEKPILKSEFQKSNKKLRYLIAYDHYILITKKPEDPPKKYLKLDFETKFEILRTQIQKKDEDDDSLGTIIGMNLMRDHGQDQMPSYTLSANEKVITQWREFFQSRINQWQFHHLFRVFKKIGKGNFASVYLGERIEDGAQMAIKAFSKQAAYAEDHGKEAIVNELTIMRKLNNHHLMRMYEIYETSNSLYVALELLEGGSLYDLIKEKVILSTKQIQQILVGILQGLCHMHEKDIMHRDLKLENILFKQPKKMETVVVADFGLATHVNEPVYLYCRCGTPGYVAPEVINIKDMKAHYSSVCDIYSLGLVFYLLLTGKPAFPGKSYGTVVKQNREATIDFTLKQLQNAPNTAVDLIKRMLEKDPTKRITAHQCLQHPYLAEMNQIMLEDNQNDFIDEGEDNDLGMRMNALNEESVKFDAFRRNQVMNSPQSSPGALATKQLKHQKNIDSSNQLQLNSPLLTGKTDSVDSIPNIGMTQQKQQNNFQASPQIKPSRFKQTIPQQKQENPLLKYTQKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VWQ6</accession>
    <name>A0A8S1VWQ6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0750194</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000075">
        <property type="protein sequence ID" value="CAD8181087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VWQ6"/>
    <dbReference type="OrthoDB" id="290672at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8181087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="135" mass="16306" checksum="E040C2768FA72346" modified="2022-10-12" version="1">MLLRKLALLYEFCQQSARQCFQFLQQDPIEQSGQISTQYQQANKDEQQLKKQQLQQDYGDLEDQDNDHHLIEQENPFEKLKPTLNFQNTIPQQNNNINSRVEKEQIKYDEEMVDIHQNSNWNNHEFDQENEHIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W0F9</accession>
    <name>A0A8S1W0F9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EML-like second beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0770013</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000077">
        <property type="protein sequence ID" value="CAD8181672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W0F9"/>
    <dbReference type="OrthoDB" id="414519at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF8">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25173">
        <property type="entry name" value="Beta-prop_WDR3_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="219"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="261"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="303"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="345"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="364"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="387"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="429"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="471"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="513"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="555"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="597"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="639"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="681"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="723"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="765"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="807"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="849"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="891"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="933"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="952"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="975"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1017"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1059"/>
            <end position="1100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8181672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1176" mass="129747" checksum="12E2F72841DF267B" modified="2022-10-12" version="1">MQGTLQENEAITDIFSQIKDVDEQLFDVVLEMLIKKKVQDCIGYLSKDENQKHIQSQIQQQQENKTQVDKEQKLSVIVNNMKQITDVLKKLKDHDFNSKDFSSKEKYGSTLSLINNIQGNSQIIKFLQFLVHLTSLDTTLIQCGSNSLHILVQLKVNLRNTNFENIKILNTSLVGANFVKCNLSGSKLDNVDISGLNLNGAQLFNCNWKNIKINELNKLDGHSGSVNSVCFSLDGTKLASGSEDKSIVLWDVKTGQQKAKLEGHTHNVLSVCFSPDGNTLASGSEDKSIILWNVKTGQQKAKLEGHRDWVRSVCFSPNGNTLASGSEDKSICLWDVTTGQQKAKLDGHSDQVRSVCFSPDGTKLASGSEDKSIILWNVKTRQQKAKLEGHSDWVRSICFSPNGNTLASGSQDNTIRLWDVNTGQQKAKLDGHGNYVRSVCFSPDGNTLASGSEDKSIYLWDVTTGQQKAKLDGHSDYVRSVCFSTDGTTLASGGQDNSIRLWDVKIEQQQIKLDGHSKYINSVCFSADGKTLASGSEDKSIRLWDVTTGQQKVKLDGHKGYVYSVCFSPDGNTLASGSQDNSIRLWDVNTGQQKAKLDGHSDYVRSVCFSPDGNTLASGSYDNSIRLWDVKIGQQKSKLDGHSDYVYTICFSADGKTLASGSEDKSIRLWDVTTGQQKVKLDGHRGYVYSVCFSPDGNTLASGSEDKSIRFWDVKKEQQKTKLDGHSGTVYSVCFSPDGSKLASGSEDKSIRLWDVNTGQQKAKLDGHSDYVLSVCYSPDGITLASGSEDKSIRLWDVKTGQYKARLDGHSGTVYSVCLSPDGSILASASKDKSIRLWDVKNEQQKTKLDGHSGYVYSVCFSPDGNTLASGSEDKSIRLWDVKKEQQKAKLDSHSGTVYSVCFSPDGNTLASGSYDNSILLWDVKKVKQKAKLDGHSGYVYSVCFSPDGNTLASGSQDKSIRLWDVKKVQQKAKFDGLSGNVYSIRFSPDGNKLAFGNEDKSIHLWDAKTGQLKAKLDGHSGYIYSICFSPDGNTLASGSEDKSIRLWDVKTGELKSKLDGHSDRVWSVCYSFDGNILASSSGDQSIHLWDVKNEKQISSTDKNYRNINTQLKALIFQNNPIPGSYYSILVISQEIILEAQGTQILQGEFINHQEKDLKPLFKSKGSCFLEDLKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W9P8</accession>
    <name>A0A8S1W9P8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0850215</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W9P8"/>
    <dbReference type="OrthoDB" id="290965at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8185677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="131" mass="15572" checksum="6D21F643305E8AFE" modified="2022-10-12" version="1">MNQRKRVKTLLDYNKIERDLPNSELTEQEKSIVGLAIRERKFNLRTRIKSVNSKAEFCKNYREYEDKMKLSIAQIVDFEYKQSRQSVSTVKHRGQFTTIFSIPKPRYDPSIVYGSQNNKRVVNIKEILKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WB87</accession>
    <name>A0A8S1WB87_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mini antigen</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0870167</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000087">
        <property type="protein sequence ID" value="CAD8186621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WB87"/>
    <dbReference type="OrthoDB" id="285118at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035823440" description="Mini antigen" evidence="1">
        <location>
            <begin position="19"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8186621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="273" mass="30733" checksum="D1E6DFF444C7A71B" modified="2022-10-12" version="1">MLFKILFTVTLSAIVVNGISFDSSKHCSCSLLSETDCINQKSWCQWDPNDKECIDIYAICESYTEAITCQPKLGCFWNMNQECAEFTSCSEYTDKQCPLGLCKVINEKCTTLQVEKCSSKTTKDECNESYDSMDSHCQWSKGSTCNSLPNIPSCGGLADFEDQCLEVGCYFENGNCREKKCSDFTQSDCLITYVSGSGNQYTYCSWNSDTKKCVEGNDVSKIVQSICNTFTLHNYRWSEDQKLCVVCDELDDNDLPNSYSIQLGIILLTIILH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WBF0</accession>
    <name>A0A8S1WBF0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0860192</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000086">
        <property type="protein sequence ID" value="CAD8186167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WBF0"/>
    <dbReference type="OrthoDB" id="295747at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="147"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="207"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="10"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8186167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="357" mass="42921" checksum="F20695A540BDE6D5" modified="2022-10-12" version="1">MSDQKKTKKPNLSSVSLTEKPSIMKQQKSPKHYKMSFDPEKYVEREDYLKMEKKYNELVQRVQALFLEINNRYYDFNDINEKLNRKSNEFGYTRLDPYRLIDLIKEMLATYIKGIIQTEEEVQSYKLCNDDEQQDYIWLLHKSEMDMRKMIRENQQLKLLIQNLEIKISNNEKQIELLTTYTQKAMEEMKSEFTFAIDLLSERESSLNILKDEIKRQTELITSQKQQLISVQVLEHKMGHLAEKQNKEKQKIKQEYDHTVKQKEQYLKQSFQLDQRAKEKIIQELEQEVKQLQIEKNIIERDLAQKIEFSQKKIDNQNNQLAEKISQISNLQKKIIDIHHSNYLQDKANHQSTKHEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WDV9</accession>
    <name>A0A8S1WDV9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0820142</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000082">
        <property type="protein sequence ID" value="CAD8184106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WDV9"/>
    <dbReference type="OrthoDB" id="307431at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF274">
        <property type="entry name" value="MYB-LIKE DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="69"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="125"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="184"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="184"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="490"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="490"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="509"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8184106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="536" mass="63413" checksum="6A079FCEC5782698" modified="2022-10-12" version="1">MNTTLQLHLPKLQQFIQNFTEDKSSIDLSKLQNTIELRIQQTSISINEVLHFYEYCQSQEFLKMHSSRNWTDEEWKILLWVTQQYCSLYSKSSEEFQGQDWNNISEIIAFKDSLNCQFKWLSHLRVAPQNKNWLPEEDKLLYKIMTKEPNIKWFEVQYEMFIQSQGIYFRKAKQYRERWNNYLNPQVNRGIWTEIDDYNLLQITLVEGLRWSNISKKLKNRTENQVKNRFKSIINKEKKIVQIPSDLLQDHQDDPLKHGLDKMTEFLIHSILSRLKPVENTKNSISINLQEQQNYNSEQSFPQIPQYSYMIPPQQYYFQQFPQIPQIQQIQTIPTIQPQLQSINPILPCQQQQLQGMNIQIPQNQSLISQQLIQQQLIQQQQQQQQMIPNFYQAQYIPQQFIQQQQQQFKFNLYSSQQNTPTNSIGTLSSQRSDKLISDQIKEEENSGSSSNKEPKVQKLVCVSAQSSINGSTSSVNSMDVIKQNFNNMHIDSEQNSPDPIQKRKHQRSQSNAFDVNNQGKIKTKRSRFFYASNDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WE62</accession>
    <name>A0A8S1WE62_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0820182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000082">
        <property type="protein sequence ID" value="CAD8184186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WE62"/>
    <dbReference type="OrthoDB" id="298344at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="159"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="448"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8184186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="530" mass="63396" checksum="A3A0E98F405F5466" modified="2022-10-12" version="1">MDKNGESIFTSEFWREINEQYFIVQSPIQIADIDILKSKSWNCKKSKCLKLLNKLIRQNRQNNRTKLFQANLNNIKTRVQLLFLQRQSTQEPIQNLMDEMIDQVCFTHLTVGLQQAEELIKNISQYSESQQLQIYNKEAILLSNLIYQIDWPDELSISIEQYQQQLNELTNNNKDALQSLEQHTHQINLTVLYTYNSQQIGEYKLKLIRQRISNYQNELNLLKQQIISLQDYQVAESVGKATFNDYNNTITVIFDKYLTSSPINLSLEYFGNLQRLREQPYDLTTQQCGLRSDDVFEMQMCHYCKEMVEVKNMKQCHYNHFSMGLHEYNEDFLICQRYQINAKQTQQYFLDLYSENYIIENQQIMCQRYFCLKCLKYDFDSYEITSYLWICPLCKGLCTCIRCERNDMIYKMKRQFLELNGDLEEIYKSSYFESLVKEKRKQLSQISLEFLKIHKNEYEENLQQKNNKRQFQINSIRKKIKKDDQHIVNKCHKLYQLESSSSSLKIKRSKETTQNTISSLDSINSQLYIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WKI7</accession>
    <name>A0A8S1WKI7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0920036</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000092">
        <property type="protein sequence ID" value="CAD8188679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WKI7"/>
    <dbReference type="OrthoDB" id="305939at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8188679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="177" mass="21176" checksum="0D17E95A68A6A39F" modified="2022-10-12" version="1">MLDQRQTLSDIKKLRNTPYIYYKQYKYVDNNQQQDSKQAKMIARPNVRHQNLSKLQQEQNDLRQSSKQRERTKTQSNYRPFSINQKAPSNSKSQPDLTDDNETIFVPYRQRNISQNSISISYDTSNKKQSFLPNMNQQDPMLQTFGKEVFIYKPKRFPTEIFQGNVSKMKKQFQQNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WUJ3</accession>
    <name>A0A8S1WUJ3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1040093</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WUJ3"/>
    <dbReference type="OrthoDB" id="303275at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="327"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="33"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="251"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="343"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8193453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="550" mass="66251" checksum="25FE6B05C98B4FE4" modified="2022-10-12" version="1">MQSPFRLYSAQKTFTCRNMDTKTSFFISKKTKQSKKQQEIDNLNEQLLQVKQSNNQLLEENKQLKQQILHIEKQLNKQEKLLILNKQNKAESKQNELDQQIRMKTEETCSIIAYQKQIKDYKAREMYNQQQIEELKRDTRRTKLQECQIECKIIKEEYDKIKFKYQALIHQIQFNQKDQLLKDNIKISQELLISQQKILELELHNKKIQQELHHSDFKRIQTEKLVFEKERELQLIQFEYSNPKKTINDLNKQWQIRLENQKLIYQQLEEEMRLRNDKINLLEKTLKEDKTAYQLKEAYYKREIDSLNIQIGQLKETIQHIEIQQMVEQSSDEQESQSEKSVKQTSQPQSKQKTIVLNKKRVQKVSLTEVETIGKEIALKFQLAQIPFSRLDSYLYSKSSKGIINLSEIDEILKQFPFDLSDDQSLLVARFLAEPEQEEWIYYDSNQTNDVVIVISIFRNLIKPFELLNPQCYDTIHQELKQIFKLNKNKLIDYLMLNGDTCDLNYFKKAFEYCDISLTLEQETYISIRMYEKYRRIINLKYQEIIDYFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WYL6</accession>
    <name>A0A8S1WYL6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1040220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WYL6"/>
    <dbReference type="OrthoDB" id="288461at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8193681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="292" mass="34692" checksum="016C9AA21E638EE3" modified="2022-10-12" version="1">MRGRILISRQNIRKNFFQKAYGQLDNNSKDPNDEMLYLKEYEKKVIENNYKNIDQENDSNAIIIAKMKSQLFKLITNINMLQKYPISNFLLLLLIMINHSYIQQPTIHMNDIAVQKDDELIQNSLKNLPRFKKMEIVGEIFALLVLILCWAFFHQSFVYLNEKVPTEFDYNGNAVRYADKNILFALPAVMTISYIIFTILQFVPHRFNYDCVGLTVFNAQEIYRTTRITLLSCKLITEFLFTYITFTMLQVVQYECEPQRMYYAFVFILPYLIIGVFYYRKLKNISTQPQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WZ51</accession>
    <name>A0A8S1WZ51_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1090018</name>
        <name evidence="2" type="ORF">PPENT_87.1.T1090019</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000109">
        <property type="protein sequence ID" value="CAD8195254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000109">
        <property type="protein sequence ID" value="CAD8195256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WZ51"/>
    <dbReference type="OrthoDB" id="295798at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8195254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8195256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="232" mass="27449" checksum="BC9B8E95CF455476" modified="2022-10-12" version="1">MNNQQKKIVDSHINLAKQLTQIAKQKSLDKFYQFGQSILQNDTLTINDYPQSEENQIEQSKEIDKLRLLIRMLLNNYNQQEFKKFEQVFQIKNEKYLNILSNFKQRVASNQNNKQQSVYNLYLLLSHLKCLILAKEERNLQLFKCQKIFFLNELKIDDLQQILTIYDSRVKNISRLEFGLLSTIKGILVCFTDGACYNEYAELNDFAKSINKMIIYGGNKIYNAEEFLLQFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X0P4</accession>
    <name>A0A8S1X0P4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CCDC81 HU domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1050139</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000105">
        <property type="protein sequence ID" value="CAD8193979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X0P4"/>
    <dbReference type="OrthoDB" id="414368at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040673">
        <property type="entry name" value="CCDC81_HU_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028034">
        <property type="entry name" value="HU-CCDC81"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14908">
        <property type="entry name" value="HU-CCDC81_euk_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18289">
        <property type="entry name" value="HU-CCDC81_euk_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCDC81 HU" evidence="1">
        <location>
            <begin position="18"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="CCDC81 HU" evidence="2">
        <location>
            <begin position="147"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8193979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="296" mass="34641" checksum="3E49A3E944807A5D" modified="2022-10-12" version="1">MNLDDIYNWILNHENNYPGKHQESQPRTISSYKPLEPKAQFHIVIKATAEYIKEKMFNGNGVNLKEFGAFTMEVISDYVKPLQHSGFKMTEDLDIQRADRKHVHTIRPCFIPDNQFKYFLQRYPGKEEVTKPLSQHSIYQKGFGMNFCNAGPIAASCYLGKDVVQSIHYSLIKAIQDLTRLGHDLHIDFGFIKICILNKDLKYKYEQSFINRLNQTDYELKMRKSDMATSQHWKTTQEEKWSKSTLNHLLTRPNPNQVQENYEKSMALKIMSLDLNTAEQTHYSKNQKIKLPTLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X1H3</accession>
    <name>A0A8S1X1H3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1060177</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X1H3"/>
    <dbReference type="OrthoDB" id="290533at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053083">
        <property type="entry name" value="TF_kinase-domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44305:SF2">
        <property type="entry name" value="SI:DKEY-192D15.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44305">
        <property type="entry name" value="SI:DKEY-192D15.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="24"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="404"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8194405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="416" mass="48929" checksum="E89AA709AE5471BD" modified="2022-10-12" version="1">MGADQSDANEMKTQQQYQMFLLQYSKERDSDHNQFGLVKLYNHNQNNSMICIKKFQTKSDTELTQITNHLRKRKIFVHPNLVQLLAVRTSNEQSICSDSSLVMVVSEFFPDTLQSELKKRRQPLKRYPESHIWLLIQQIVDPLAFLEDQKQVHGDIQPQNIYLDETGSVKLAEYNYFPGGQYGFQKMLLSKDRAYLSPILLNNYRNMNFKAQHNEYKSDVFSFGMTLLEVLLLEESYDCMDFQNGCIIQNVIDSKLQKVKISNYSQLLTNFVRELIQIEEKLRPSWNDLKQVIDQFRDKICNLIPFYQEKRQMSPTKLVYQSTYIPQQHILSSPQSKILSSPRQPQMLYQTYLQPQPLYQTKQMQPIPMQQSIYYESGQKQVPIKKITKLEDTVSKQVQQPENNSEKTTLKQSETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X1L1</accession>
    <name>A0A8S1X1L1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1060207</name>
        <name evidence="3" type="ORF">PPENT_87.1.T1060208</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X1L1"/>
    <dbReference type="OrthoDB" id="5946895at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010300">
        <property type="entry name" value="CDO_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05995">
        <property type="entry name" value="CDO_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="367"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR610300-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8194463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8194465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="436" mass="50681" checksum="7033D2F8FCE1D6B5" modified="2022-10-12" version="1">MSTEDAVFQSQTLDKRIDLEFEQDEKVIIIEINPQKVLKEFFAQFQLLNRIIVRYLNYSLQTCLEIQYGRNQLLISSQDSRVLLEDQKIVMQKNKLDQIVDTQGIWIYLNKKTLNLNIGYGEARKETIVAHYIMESVQNSIKSIKQITIWQESIPLNFLPQIISSQKGELKPIRIHTKDHFIPTLSSVILQKCQNIFDLNQGKQAKNLASEIDDLYQQASQIELHQIEYFESGQQVQGVAKFNLGDLIHHSVTIEGCKLNQILKNKQETTQKNGLDPLMTYIRLGLGHFNKDSAGHQFVLEIWPFKHYSSIHDHAGQYGIVKVLQGIINVKIYAILSNAKHLMNPIKSLTCNQNDMTWLSPKLNQVHQLNNFYPQTAITLQCYKYSKKSQEKIKQFAYVANQQYKNFNPLSDFPDIELHTIFKELVEEYSNLYKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X8J1</accession>
    <name>A0A8S1X8J1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1150001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000115">
        <property type="protein sequence ID" value="CAD8197236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X8J1"/>
    <dbReference type="OrthoDB" id="29145at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8197236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="126" mass="14968" checksum="526487B3CD8F41F7" modified="2022-10-12" version="1">MIPALRIKGNILSGNKTQSDHILKTEVLSRFEILFQHKNKDSKSSQVRNQKTNSFYLTNSVINAELDDWIILLCHMNLLKNYLTIQIRIIKQQFKEVFRGFLNLQKRSQLQIIQFNMMKNNQFQKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X8Y2</accession>
    <name>A0A8S1X8Y2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1150089</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000115">
        <property type="protein sequence ID" value="CAD8197404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X8Y2"/>
    <dbReference type="OrthoDB" id="285774at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="437"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8197404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="636" mass="75598" checksum="44495503FF575578" modified="2022-10-12" version="1">MENEQMCKNHPKQTLSWICLERNCQQRVMCSTCAVKLHDRNHKLEELNNIYENGCLATYFSNKKNSSAGLLQLINGEQDNVFENEQYDDYLQEGIERCLRNVSKKIEETKNQLIKQHEKLKEDKINERILFIDQLNELDRKLKTSSNLEVDIEYAFQQIQSFAQKKELEMANNKESAILLRKIRLLELMKDNFLKMIKSYFHNQFTAEFCHLFDMQMETLNAREVDDVQGFLDYMDSKWKQDYRNIQLSIEKFVARLNEGLKYCIGMPLENPKKQLIVRSPMRQFPINIQTPTYQHFTSPKQVEFTKSQVIYEEPSQRKNIKIEPVSQILFVAQPCKEIQRSRSQSTSIFKDQQINGIIQGPRYDNRGEVIKRSIVGTVDQFQREGKVQYDSRVTDEESSMLKSFVITQFNKKQNIKKKIEPIQLSKISKEEILLHIDKVQNNIKCSQSSLRSQEETLPIIERNLLTKQRRILESCEEQQDKWNQLEQELANRCERSHNNTLIRRSQGYREKKQLLDSLNVLQKEPNVKEWYAKLRNYNQSIEPSIEIIKNVNHVKMYSEKFKNMINQRLNNKYYDADYLVVNGNSKLKMESKEATDLMFLPQDIVQQQQNQDFNYSNYNKRDISRRSSYRIIQLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XB73</accession>
    <name>A0A8S1XB73_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1180043</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000118">
        <property type="protein sequence ID" value="CAD8198268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XB73"/>
    <dbReference type="OrthoDB" id="2620952at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="139"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8198268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="542" mass="64167" checksum="FF73FDF35EA2A221" modified="2022-10-12" version="1">MNQLFTIANHFFIDKNNKSTSFWDKDFRKLLQNERDFTLNNEHCICLYKKGKFLNQFIERYYTIQGAYIFYGYNDLKGFRKLENVYMRTAVDSNSYPYQIQLIHNQSQVILYSNSEQNYNQIRNQLEQYCILIGFHTQYTIINQIGFGSTAQVFISKSQVNGQCYAIKRIRKNSKLNPQFLMEEIQIMNELSHPNIIKLYKVFETNKHINMVLELVEGGELLKSHRYNLRDARMIFKQLATCVDYMHQKGIMHRDLKPQNILCKTNTVEIVLADFGLATWIKDKQHIFYRCGTTGYVAPEILKYKEGTKMYTEKCDIFSLGVILYQLIYNNHPFKDIDKTKVLQKNLNAEFKFDDTIKVPQSCKDLITLMLKQSPKQRPTAAELLRHDFFNEQLFEQSNSNLTMIIQETYSDIKRSGPSLSFQNVDMKFSTINNGFKQFEDEVNDNIEQQQGTDFVELSPIWTKRIDLHRCSQNQQQGNQMVFQRQYSQDIKGKKSSIFRSEFKQKSIQMLQPLLEISQLDFQDYHQIPYINHNNMLLIHMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XBD9</accession>
    <name>A0A8S1XBD9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1180094</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000118">
        <property type="protein sequence ID" value="CAD8198370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XBD9"/>
    <dbReference type="OrthoDB" id="311822at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035917891" evidence="2">
        <location>
            <begin position="18"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="104"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="190"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8198370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="261" mass="30208" checksum="BB9AFC19B126B40C" modified="2022-10-12" version="1">MLFTFILLGFVNSSCLTRNFEECLSLNVEYQESCQLSQGECRPRPEKCTEIDATYPLNCRTNECYYSKRFNKCLSQPFTRLLNEVADRGDHHDHQQDSHDGIDQQRPQPTEQGPLPPPYQPPQNPPQQPMPPQNDDQIEQPKEEENNDSLEDDEEDTYEEEEEVEEQDINQNTSDEDESWNEPEVPSQPNSTNTTNSTNNTNNSNNNDSDGFNDTYEGFQNIIEEEIKFKLSQSDENKQIEIKSSGTNHLIMAFITMIIWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XC31</accession>
    <name>A0A8S1XC31_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1190045</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000119">
        <property type="protein sequence ID" value="CAD8198528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XC31"/>
    <dbReference type="OrthoDB" id="284370at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8198528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="66" mass="7781" checksum="60F2286486E27248" modified="2022-10-12" version="1">MNTHTITYPKPLIRIMQHSKTDCSSVSSTQSYESHFTDIERPENPMIKNFVDNQEDDLDNFFDQLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XEI8</accession>
    <name>A0A8S1XEI8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GMP phosphodiesterase delta subunit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1210125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000121">
        <property type="protein sequence ID" value="CAD8199329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XEI8"/>
    <dbReference type="OrthoDB" id="10248777at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008015">
        <property type="entry name" value="PDED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017287">
        <property type="entry name" value="Rhodop-sen_GMP-Pdiesterase_dsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12976">
        <property type="entry name" value="RETINAL ROD RHODOPSIN-SENSITIVE CGMP 3',5'-CYCLIC PHOSPHODIESTERASE DELTA-SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12976:SF0">
        <property type="entry name" value="RETINAL ROD RHODOPSIN-SENSITIVE CGMP 3',5'-CYCLIC PHOSPHODIESTERASE SUBUNIT DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05351">
        <property type="entry name" value="GMP_PDE_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037825">
        <property type="entry name" value="GMP-Pdiesterase_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GMP phosphodiesterase delta subunit" evidence="1">
        <location>
            <begin position="16"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8199329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="156" mass="18419" checksum="614EFE59D5D5020D" modified="2022-10-12" version="1">MEIQKKKRELTAEQVQKIKDGFQIQRVIMKDSETQQIYWDSITEKITQDLIDITLDPKIITSKAATRSIQFSTKEKLQDLILIQDAYLHDKKIEHFVFKFGFVMPDTVNTWDSTIVNRPPEQMLPKEIQSGNLIVDIQMFEQDHLIFNVKVRIFYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XGH7</accession>
    <name>A0A8S1XGH7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1230087</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000123">
        <property type="protein sequence ID" value="CAD8200031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XGH7"/>
    <dbReference type="OrthoDB" id="312324at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06614">
        <property type="entry name" value="STKc_PAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000768">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF18">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DST1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="179"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8200031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="457" mass="52476" checksum="11E39067169B3EE2" modified="2022-10-12" version="1">MGQKSSKEKNQHTPDGQISNFQSHIQITRDPITGKLQGVPKEWAELNGLKLEIDQNKTVETKYLPASVQPSELPEAILDLINEPIMSAPFNLQHKIHIEIDPTAQLGLKGLPPEWIEKLKKADLQKADIEQNPQVMIQIISNYEEGVYRQTKLTLPTNDEFIKQVMDIKFIEQDPSLLYKFTEQLGKGAMCKVYKAIHRNTNEEVAVRVMKIGNDMQRIKVEIALMKMCANQNIVKYYDSYIYQQCLFMVVEYLDGGCLTEIIYQNFKSMKEPEIAYICGEILSGLNYMHKKKKIHRDLKSDNILMNKKGEIKIADFGFATQLTAERQHRKSVVGTPAWMSPELILKQDYDEKVDIWSVGIIAIELAQGEPPYLRVPPLKAMYSITANDPPRLPNKFSKQFQEFIEKVLDKNSKTRLTAEQALELPFFKNRNKDGVLQMIVNKKNIPLNELIKQAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XGK1</accession>
    <name>A0A8S1XGK1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T1240079</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Plays a fundamental role in microtubule organizing center structure and function. Component of the infraciliary lattice (ICL) and the ciliary basal bodies.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the centrin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000124">
        <property type="protein sequence ID" value="CAD8200356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XGK1"/>
    <dbReference type="OrthoDB" id="410571at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000178">
        <property type="entry name" value="Calmodulin-2 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF59">
        <property type="entry name" value="EF-HAND SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="37"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="73"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="110"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="146"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8200356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="181" mass="20338" checksum="321C390BE51A4945" modified="2022-10-12" version="1">MARRGQQPPPQQAPPAQKNQPGKFNPAEFVKPGLTEEEVLEIKEAFDLFDTDGTQSIDPKELKAAMTSLGFEAKNQTIYQMISDLDTDGSGQIDFAEFLKLMTARISERDSRADIQKVFNLFDSERAGVITLKDLRKVAKELGETMDDSELQEMIDRADSDGDAQVTFEDFYNIMTKKTFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XGK8</accession>
    <name>A0A8S1XGK8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1230045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000123">
        <property type="protein sequence ID" value="CAD8199979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XGK8"/>
    <dbReference type="OrthoDB" id="289458at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8199979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="137" mass="16378" checksum="46E0E2263F74FE15" modified="2022-10-12" version="1">MIFSFFYSSKNRLDRKRLPLQQTRSENQSPSNYPHIYQTQPRKQQICVQIQKNDKSDETDEEDIKSSRDCFLQIGTSEASKLINHKNRIKLRSVSMQKKQIQYMQFREFDNLDQIAEEQLQLQFQNLAVHVGQFQHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XIW2</accession>
    <name>A0A8S1XIW2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1260163</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000126">
        <property type="protein sequence ID" value="CAD8201131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="298471at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="375"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="419"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="673"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="702"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="755"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="880"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="955"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="989"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="547"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="547"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8201131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1303" mass="153533" checksum="632FFB8601A6EEC4" modified="2022-10-12" version="1">MIIFLFILQTYQLRFNITTPEGNPSEFITLQTYQTVKLKVEIGNYQLNQEKILLYITDDISHEITKSCTLKLMNRQVGELKIYVNATNTEEKSILTSIAISAIIISPARGILTQPNLFPIILSQKLIESYMMPFNLPTNKIYNLDIINDDLEKPPQNLTPFGQEFWQEYQEKDISIIALTWIPFMGNCKDQGYYLYFYNLLENATFCNLVAQEDTKVVNMVPTNGFNAKGDSCNINLECYFLENLMIEYEGNKWWQSEQDSLFYISKYALNYKILAENSDDSKTNYFYNMIQAQDESLIAVQFITSGVIKNSYPTEFTLQILYNQISQTEKQIVSAIITASNYQKVTSIINKYTFNIVYRPMDYLELINSFQFDITLYLVLYLVIGILINIGIFVIWVINKSISIIPDPPKLRIYQTFIVAFLPPITGVIRATIPIIIYVLILDNLFAPDLFSGITYNYDGLSTDFDNEQQYQKGRVGLMLVVSGIIIMKIACKLCIPKPIDEDWGIIRSNIEKKNEEKYYDFSISEEESKESPMKQDEIREAQVTSKNVENNLQSNSISRQGSLFSKQQPIPGSAPPMTKEKEPTMPKNSLFKKATILKQSSSIKDNQSLKDSISPVQLLQRQNSSGNPIDKKQSIFNQAQQSQQPQINQNQQADEEDIIDKLKDSLISKDYHYQLICIMIAALLLFFLGFSYEQLFYKNIYYFLIVLQFIESFVYWLLSEYLLEEQTLIQPLMACLEVVQFIMFMAAESFQEFVISFVIRLSLIIVFRTYINPVIRNFKHFKKKLMNFMNKWQPFFSLESEQEKQPEDNISFYLGQHTDSKFTEDQSIEYVIQMTLGFSSKVHAIFIKPIVLVFIKLFEKESQITNNYYIGFREFNYYIYFAFFVIIPQIFIDIYVLNSLEMIYGFKLFDYFDYCKYRNDFRIQKFFEHSLTYDRSIDAQYRSIDNMKFTDQFYYNGSLVIYSILLCILGTTIMIRNNYVLYQDPAIFLIILFIYIFITLLQNMMLYIWNKIDVWKIKDTKQKLNLDSLEEFLNPAKENIEQIIEVDAFKKHFIKLNKDWLVSNLELFINVDQFTQHDEFLLQTYQLLLNDERKAQKEIIRNEQMIKKQKELELKSKKKRRKTSQSQSIIKSQRDIEEIDGYQHTIRIKRSQVENLRKILSIWYYKAKQTLFLKYLVSDVLAQNQAEHCKRCGLDIELRVVELIPFEKMSNQFKYRIMGTPFNKFEWIKYYETHQKFVTLCIDCENAYKSRFLQRQNQSANDVAKLASQNFVAKAFVFKWLYAARARMLHKKSQTVEKLTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XQD9</accession>
    <name>A0A8S1XQD9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1330157</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000133">
        <property type="protein sequence ID" value="CAD8203355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XQD9"/>
    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000205">
        <property type="entry name" value="DnaJ protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050817">
        <property type="entry name" value="DjlA_DnaK_co-chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24074">
        <property type="entry name" value="CO-CHAPERONE PROTEIN DJLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="3"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8203355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="115" mass="14022" checksum="71DD52BB68D3344E" modified="2022-10-12" version="1">MKNHYITLGLDRNAQKHQIKEAYHKLALKWHPDKNINCRAQAITQFQEINEAYNILSKSESKKKYDYKLEKDDDFLEQIDKMNEEFTYLNKDDYNTLIKFINRIDKQSKRIRKHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XRK9</accession>
    <name>A0A8S1XRK9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1340146</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000134">
        <property type="protein sequence ID" value="CAD8203730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XRK9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="25"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8203730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="187" mass="22495" checksum="9B7DC3121C3A253F" modified="2022-10-12" version="1">MQKNYFLTGLILCKILNYTKNKRALRSFLLERQQLKQEIKIKEQMFQKIRRQIKKSFKNQSRQKPFFLDIYRNKVKSWKINNEISLDVTDILLNDVPKQFFTIDLDWEASQPRVENMRISSLKKEIEGFKLTYLHFQNLDNLDILDLVFGSGYVSLLLFTLALKRKKMLQIQLHLVDHHHHAIKFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XS39</accession>
    <name>A0A8S1XS39_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1360017</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000136">
        <property type="protein sequence ID" value="CAD8204101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XS39"/>
    <dbReference type="OrthoDB" id="310737at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="376"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="83"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="314"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="378"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8204101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="395" mass="46792" checksum="6BBA4D3AFE900C84" modified="2022-10-12" version="1">MQTQPNQLNTPSTQFTLRTPEKNVITTPHQIQSDIYTNTPIDSRRMIIFPQQSNNYLLGTLIPYQDVYVDTHNPIQKQPTLSMANIEENLKIITQKYQEISNQLKVENQERIDIENKFQQLQSQNKELKEELIKQKQICKQMENFSKELQQKLQNQVTMISSHSSIENQNQQNDVRQKEIEIIELKQQLDNEKLKSSNESDRLMTRFNIIFQEQKNLNQKLLQENIDLQKILRQIDNNQKISQKSTSNLLDESQLKTLVEQFYFDNNEECLLNIPNEQQLQAIKILKLISTSQNQNQQNPPIKQKEEGKTESKLNIKQELKEKQEQLIAEIKNKMGKFIEQKNVQQKDIMKFQNESEELKRQLNQIENLIFEMEQSPKHNPNKNSCVSFENNNFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XUP8</accession>
    <name>A0A8S1XUP8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ras-GEF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1390132</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000139">
        <property type="protein sequence ID" value="CAD8205089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XUP8"/>
    <dbReference type="OrthoDB" id="292372at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GEF" evidence="1">
        <location>
            <begin position="285"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8205089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="488" mass="57834" checksum="5DC80D0A2F9893DA" modified="2022-10-12" version="1">MRKQPLKLLLEDNDLEIIKAKTFDSQSQRNGTSFYSNISDEHEQFSDGKRYQCDSSYSSSSLHSSRTLADETLLQNYQKRLDPEILSEQARFVFLIGNQSTEEWLANFNEKCLRVNSFFKESLFNSLVETAFNSDDQKMKRKLVLTLDYFTNLITFTFFLLWKWIQNENQQDMSQFVQNSNSITEFIVLMITLRINEFRIEWQLITMIEQIVQPFPEKQIFKECSETQRSYLSVGLFQPKITSIRVKPNSVLPWTTDEVAFVLSFMSQSFYAGLQIKNVIVKGGLNNYFRRINTISQFIIYSVVRSPNKHDRQDALRYWIELAEKLQKNYDLEGLFIVYKYGIQLLLKDYIGTMPILFRDQSRIPRINAFYEEHIKSNFNEIQKNEQLFYIPSFHKFITQIKRLEQQVKMNNTLFDQISDILLQLVSISKRQYKLHQQTSAKISDHEEQIIQYFIKGIEKELEINLQIPLDKETLVYIELIKLAKITN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XW03</accession>
    <name>A0A8S1XW03_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1380019</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000138">
        <property type="protein sequence ID" value="CAD8204642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XW03"/>
    <dbReference type="OrthoDB" id="306500at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd15841">
        <property type="entry name" value="SNARE_Qc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="3">
        <location>
            <begin position="181"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="205"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8204642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="277" mass="32598" checksum="7567C628F89E0353" modified="2022-10-12" version="1">MRSQRIVLLLERLYSMDEKLGGNERKKNVKNPQKEFDQMIDDLKEEIEMISAKQKDRDKRNQKYGNDEEAIRLGVEIRESIQKCEQQYKSLEKSLLRQLKKPQKYNQQDLELRVTSFKVLKFALKGTKMKEFGVEEQEFKGVTLNELKNQVFEGGTHSQQNQLEYQDHLSLEEQEAMQRWNLNDEQMDKQLDEIISGLDQLHYKVQSMSQKIDQTDGQVNKLIKEVDKTNKNLNNANQALKKLTQVYRRPNKFVLDIAFILLLLGLVATIITTFTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XWU6</accession>
    <name>A0A8S1XWU6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">endopeptidase La</fullName>
            <ecNumber evidence="7">3.4.21.53</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1420128</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Hydrolysis of proteins in presence of ATP.</text>
            <dbReference type="EC" id="3.4.21.53"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.53" evidence="7"/>
    <dbReference type="EMBL" id="CAJJDO010000142">
        <property type="protein sequence ID" value="CAD8206016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XWU6"/>
    <dbReference type="OrthoDB" id="2411602at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19500">
        <property type="entry name" value="RecA-like_Lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000324">
        <property type="entry name" value="Lon protease homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000021">
        <property type="entry name" value="Lon protease homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004815">
        <property type="entry name" value="Lon_bac/euk-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054594">
        <property type="entry name" value="Lon_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="ATP DEPENDENT LON PROTEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22667">
        <property type="entry name" value="Lon_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001174">
        <property type="entry name" value="Lon_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="8">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8206016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="701" mass="80271" checksum="91F0AACD4F86ED1F" modified="2022-10-12" version="1">MQYLNLYIQPISNTVVFPYSSLQLYDVDCQIQLVHSQYIGLVSQIDDNQPNIQTISQYSQYGTLVHVVTKQEESSNYCKAFAFARFRINSFCQLSPFLIANVEILNDEIQETDTEILSTFKDAVKMYMENFSLLPNALLKQKIDEEDNIIKLYYQVANRIQIPFNQKLRLLQMNGNIERISTMIQYLNHKMTQYSTSNELEQKLKQELMSEMQNNGDNLINQTEQIVPQIQEQELDYQKQYVQSLPWGIIHPETVTISQCKEYLDKRHYGLDMAKQRILQYLAIKFLTKSTGSILCLHGHPGVGKTSIAQSIAESLGRPFQKISLGGVSDEAQIRGHRKTYVGAQAGMIMEAIKKAKCVNPVILLDEIDKINQHTVGSALLEVLDPEQNKTFMDNYVNEEFNLSQVLFISTANYIESIQPALRDRLEMIEIPSYTIDEKENIAIQHLIPKQQELNGIDGKIDFSKDVVRYLITNYTNESGVRQLERQINSIFRKIALQQVRGKKKGFYNINKGFINQCLGESTIGYKNIKLQSIGMCKGLNDGVNILEVIKIKGEGSLFQQSKDQYIVEQGITALSYLRNCFKNIQFKNWDIYIANQNCLGVCLVIAMVSLFMNKKVKSSVAFAGELSLKGFIFRQDNLRDSIIQAAESGIESIIIPSQNVNEIQNLESSIKKKIRLIDHIEEVFQLAFEDGFTYQHYPNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XYJ0</accession>
    <name>A0A8S1XYJ0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CHAT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1420129</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000142">
        <property type="protein sequence ID" value="CAD8206017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XYJ0"/>
    <dbReference type="OrthoDB" id="300981at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="120"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8206017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="719" mass="85811" checksum="95FD3F05A98D1734" modified="2022-10-12" version="1">MKTPFCVRLQILGIQQNDELEYFENPEFFEDSQHIFPLNIYAQSYGIYVIRLDYYIKKNFIKEEKIVKGKLIFTFYNYITQQFVDIYDLLCTDNNFIKLDGQFWFKDENSISPLIIRLKLQIYYQEYLKYQNLLENIELEKNNLQGNIEKAKTELRINNENFVNFKMKVNQLKQFYQIQPQYPEIDIAILYSHPLVNQNEKRVSPVQYYDDIQNFKQRISALNKKVTYLITQATKENLRQVLKYNPKIIHIIAHGENDTNKLNQYLQFENNCTDDFVYREDLQQIMKESKKSLLFLACCYAGEIAKNIQQYATTIAVDKELKMLDDAGIYYFSSLYENLLSKKTLQTSHEQAKKNVEEQLGDKNFQCCHCHSHREACKEIFGKYNDLCFKHKSQCKCEESDKINDKLSHLWGPNYKCQDICEVILNQVQEQKNKEFVNIKLDEFAQDGILRVCCCELAEKLQDDVSTFIQHTESEKFQIFSQNSTQSQIFNNVEKGNLVEINQLSWEEQYLIAWKKDAKVIYNKLTETFYSKKRQILKICAGTSKGERFVIKFAHQVSKYFKFRKLKFYKRNEIEDIQIIEIPLDKIEQLLLKKYETRISYIFIICQLQKDLFIKSIEQLLQLNQKGTIILISYQELFELDLDSNFYKIQLKDWVNNSDKKEKCDYYKILNEYGDELTQKIIETVGEDKIKKLKFDQLLDQIEIILKNQQEQINQFVQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XZU3</accession>
    <name>A0A8S1XZU3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Fructokinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T1450145</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ROK (NagC/XylR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000145">
        <property type="protein sequence ID" value="CAD8206966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XZU3"/>
    <dbReference type="OrthoDB" id="10260668at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd24067">
        <property type="entry name" value="ASKHA_NBD_ROK_BsFRK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000136">
        <property type="entry name" value="Putative fructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000153">
        <property type="entry name" value="Putative fructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051804">
        <property type="entry name" value="Carb_Metab_Reg_Kinase/Isom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000600">
        <property type="entry name" value="ROK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049874">
        <property type="entry name" value="ROK_cs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42742:SF3">
        <property type="entry name" value="FRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42742">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR MPRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00480">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01125">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8206966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="287" mass="31762" checksum="379658149CC37BE8" modified="2022-10-12" version="1">MQQSKAALEIGGTFIQVGIGTKIDNKWTLTNKKTFDTRDPEDTLIDLVTYLQQFQFDSIQIASFGPLCLNKDDPKYGSITSTPKLKWQNFPIAARLSQQLKKPFAIDTDVNACAMAEFMLGNHKVRQSLAYITIGTGVGVGIIVNGQCVHGMLHPEGGHILVAKKEEDKDFKGVCAFHGDCLEGLCTNVAIAKRLNCSITELPNVSDDHPIWELIGFYLAEACQNLLYLLSIEKIVLGGGVMNRKLLYPIIDKHLRSLVNKYVEIPENYIVEPEVEDVGLIGALLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y1B9</accession>
    <name>A0A8S1Y1B9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Oxidation resistance protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1480074</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OXR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000148">
        <property type="protein sequence ID" value="CAD8207709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y1B9"/>
    <dbReference type="OrthoDB" id="26679at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF62">
        <property type="entry name" value="MUSTARD, ISOFORM V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LysM" evidence="5">
        <location>
            <begin position="79"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="TLDc" evidence="6">
        <location>
            <begin position="351"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8207709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="516" mass="60724" checksum="1B92CE98EE4235B2" modified="2022-10-12" version="1">MLNIFKNVFSKKQKQDSQIIQFEQFDQNQLEEHQIENDFPHDFQIIDEQAMKIIEQEYDIKQQVQPEYQEDQQFKEDLMDYEVQVDDSLYGMAIKFSVCEDYLMKINNLSSDLIFQGQIIKVPKKNEQTFSVVHIQEQKQQIEYLWIPDANSQRFDVIYCNNIQNVQGQLTLSSDLILFNPFQQDYIENTHQKLRLQASISMKDINEAVYYILPNKNGHLDYIVQILLSGIGKPNFEQKYRKQLDRLKEQKQSIATVFFRHAERDNTGKLYTEEIKKENCIIMARFITEACSNYTAIVQLTQLPFIDFIQDTTQKQNLDIDVDEMQDVVGEPMGNLWASLEYVPILKEKSNCFTDTTFKYVIQSIPAIYRLANWNKLYDIDVDGSCYQNMLQQIRQIFPMLLIIKDFDLNIFGVYISSEIHKYFEGFKGNGETFLFNIDTIKNQIKQFVWTEKNKDFIFCDESGIGIGCGDKFGLFIDQSLSFGYSNPCSTFDNPRFTKDEKFGIKNLEIWAIEQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y504</accession>
    <name>A0A8S1Y504_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1450084</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000145">
        <property type="protein sequence ID" value="CAD8206844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y504"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8206844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="193" mass="23315" checksum="F759912568C2E061" modified="2022-10-12" version="1">MQEETFQFQQQQVIEYSHRKILKPLQRKLQYKSQDPEEIYSTQEPSLDCNVISIFSKRGTNFSIEEEHHRRIIQNPFVNEVPNFSKLQYQLNYTIQQQPEKTYAPIDILQNELSNQNYSNNKYQNLQNDCYQPIEESFGEDYRLIQMDDFNYLQNFQCNKIENQKNDSRNKKQIPKKLQQELTKGIHKILIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y691</accession>
    <name>A0A8S1Y691_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1540082</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000154">
        <property type="protein sequence ID" value="CAD8209359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y691"/>
    <dbReference type="OrthoDB" id="292525at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052798">
        <property type="entry name" value="Giardia_VSA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23275">
        <property type="entry name" value="CABRIOLET.-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23275:SF102">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_170, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035847680" evidence="1">
        <location>
            <begin position="16"/>
            <end position="710"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8209359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="710" mass="75511" checksum="CAD6CFECE60A3CD6" modified="2022-10-12" version="1">MKILFCIVLLLVVKGAIIVTCPKSGYGCVTYGANTIIQTTRTNSLCRNGYYWNNQACMPCKNADIVVGGYYTCSAYYEGTSLTCISGYTLINGVCVQIPLGCTAYGLNQNSDAYVCTACTSDYTLKAGVCINNRYCSSWDDNAVCTSCQTGYFLNWDLKFASISANLNNQSDWINTWQTYYKFPGESFCSVCSQTMANCKSCNSAYTCSQCNDGYYWQQSISSTQLTSSNAQFAGSCVSCLTSGNCSTCQGSSCTQCKTGYYPTKDTTNTVILSCNLCPNGCLTCTSSTFCNDCYASFYKYTDNLCYPLNKCASINTQLTTIPTTPKCSSCIAGYALDPSGTGICYQCGNCDSCTIPSGQQTASLQTSCTTCWDTYYGIKDTNGIVTCKPCTQASDSFLRCQGPVENSSLTGVVPTQCQDGYYLYTLPASGTTQAQVVCVPSNQTQQCVTIVATSATQYTCATCTTQTKLYTNSQCLACDAGLTNTLPQGCTACTGSPTNSIACSACAVNYYFSTGTTTTCTACDVNCLQCAAGPTCTKCAITYYVSGGGCTQCGVANCATCNAQTPSSCQVCLDGYFLSSINTGTVTTSYCLKCPFECATCSAPGQVCNSCISGYVLQNGGCVTLSSANCAEGYTASTLTNQQNPTTQNGCAICKYGFYNFEKRCLQSVQPYAGAVYVPKNIGIGSNPGVSNYETIMYSIFLIIMLLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y6E9</accession>
    <name>A0A8S1Y6E9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1540108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000154">
        <property type="protein sequence ID" value="CAD8209409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y6E9"/>
    <dbReference type="OrthoDB" id="283959at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8209409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="364" mass="42618" checksum="403ECD42B6D40C7F" modified="2022-10-12" version="1">MALIHNQNDLQNMFSKAGKITSEKYIVFQHRDDHPVKKFITPQPSKSPDSIKKKPLSQQESLNETEQNQLKQLLKLSQPFSKQISRQIGHYTQIGNNSKASCGQYHIKYSELDKAVHSVPHYDKTLKHTMPIEIPPKHQQDLPIDTPSIIEKPKVKYESFVDLQRQIPRADIFSGRPNPHPDRFQYLNVTNQWSKIPRQPQIKLEKQLSRDQMLIYKKKEFAPDYKPNFEYGKKQLGSCGAPFYKLEQRKDIMTKIPPYTFESYFEYDQYSKKSKSQLFTSPTAPNFKTMLDREYDQRSLLPSFMQKFTNTRMGITHLNQKMLEVNNFKDGRFLTVTSSFMPTIQKQSKKSVNKSSQNQSIDEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8H6</accession>
    <name>A0A8S1Y8H6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPENT_87.1.T1560082</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000156">
        <property type="protein sequence ID" value="CAD8209860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y8H6"/>
    <dbReference type="OrthoDB" id="354826at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000210">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000524">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="61"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="357"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8209860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="387" mass="44211" checksum="C79242A6ABBDE7AC" modified="2022-10-12" version="1">MGNICDFMKTEKKEFQSSLNQQTVLQPIQEQELAIKSSKTLDNSTSFMPEQNQKRVGLEDFIMLATVGKGAFGKVYKVKKKDNQKIYAIKCINKKLIFDSKLESNALLEKNVLKQSKHPFIVQLKYSFQTPTKLYLVMEYINGGEFFKILMKTKGLPESIVAFVAAEVVLALEYLNYQLKVIYRDLKPENILLTTTGHVKLTDFGLATLRKDENVKNYTLAGTPEYLAPEIINKKGHSFEVDLWTLGILIYEMINGYPPFTVQDRNTQKILQLILQNKPNYPSIMSDEAKNLVQSLLKDNPKERIGAQIGYQEIKYHPFFANIKWSELYNLNIKSPLKTFAETNASRSEGLRMPNIQIQETPNPPQPNLQGISYAGGDDTYNSKRLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YB32</accession>
    <name>A0A8S1YB32_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1510121</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000151">
        <property type="protein sequence ID" value="CAD8208664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YB32"/>
    <dbReference type="OrthoDB" id="285020at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8208664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="170" mass="20775" checksum="F10DAA9AF74C6C9F" modified="2022-10-12" version="1">MMQKQYELEDYLFLYERSINLLTQELSTVQEVDQNSYQHHLEDKKQIIEQSIKVDYKNLIQILIFIMFHQQPIIGELINNHVDIVNQLIKQKEELCYQNSKNMDAFADCMLIKEKRFKKLQMKSEIFIRFCELKYENCLQEKDEQSCIKRINNLFDDFNYHMKYENVQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YBJ6</accession>
    <name>A0A8S1YBJ6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1630087</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000163">
        <property type="protein sequence ID" value="CAD8211445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YBJ6"/>
    <dbReference type="OrthoDB" id="277011at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000121">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="2">
        <location>
            <begin position="205"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8211445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="410" mass="47870" checksum="00E2B7E0ED5FCB8B" modified="2022-10-12" version="1">MNLSRNGSNKFSRHDRIAYSQILDLKKNKIYPSSTKNSDQSNGYFLSLVNMKAPDYGQAQLLNNDALKNKINEKRNQKSMDNELNKFVTRVETKLISSPLKKKVIAIDTKIDIPTQIKPYSLTPQSRLQSFRIASPSQKPRSSSQNKRNEIYYLSKLQRAFDTNGSDYFSRMYRQHFHQTYQGLNCRLFSQNNNDNNKSHKLPKKHQRQFTLFFDLDETLVHCNETPSIPCDVVLEINVSKHQIIRAGINVRPYAKEMLKNLSNYFEIIIFTASHSSYAEKVCNYLDPDSNIISHRLFRDSCTQTNTSLYTKDLRLFCENTNRQLSQVALIDNASYSYAWQIENGIPILPFYDNKDDRELIDLEKYLKNMIGVSDIREYNRNNLKLHLFVDQRGPYKVLENLFGKPQQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YEF1</accession>
    <name>A0A8S1YEF1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1640059</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000164">
        <property type="protein sequence ID" value="CAD8211598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1926336at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000793">
        <property type="term" value="C:condensed chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43941">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43941:SF1">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1442"/>
            <end position="1477"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="45"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="238"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="434"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="684"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="774"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="904"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1008"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1116"/>
            <end position="1157"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1189"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1297"/>
            <end position="1423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1442"/>
            <end position="1452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1460"/>
            <end position="1469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8211598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1491" mass="176138" checksum="8B5762BFB07302DE" modified="2022-10-12" version="1">MAENIIQQKTEGDERASVYFGQEIDGDKIIYKNVRKAKIPNTADIAYCEIRITELQAELDAQREQVFKLEQQQDLYVTYEQTINQLQERNQILNDENKQLSLLLRSRTKDLDVAKFKILEYESHLERLRDVEQDYNNTYDQLQVRDKELKELTEKYGQLELKHSEQKVLELENFDLRNKITNLQNDLNVYKDRNIKLEAERKRLADLQSEVSTTSKIEVTKSEVNISQVATIQPAQDYAKYKTQLQEQSDLLNQMKNAIQEKDHRLGLLNHELNKLQETLSNKNEQISQLESQLDQSNAKLKELEGLTQQQKTKPTTIIKTVEKIVKVTTTDADEEKVRLTNELERANFEIERYKFELAQLQQKLETQEKIISDLSRDVRQIISLNEEIELLKIDKANLLNALNQKEQDQEFTLNLLNQKTIELQNLDKIKFNLTYAEDQIQTKQREIISLKRRVGDVENSNNQLKQLQPELRQAKKLIEELRTENENLDQQIKQLKANLMDAEVVINQLKKLEQRIKALQNTNSSLRQEITQTLAESEQWRQKYNQGCSEITRLQRLLEVNQQRQQILNEQNKNFKNDFEVPDIKVDPLPIPQRLSKQIIQDESQITKLKNNLVAVELQLESVYRNKILEQQTYQSQIDEINSKYQLKKGESESWKQKYLKSEQENVKLMNINEKYNVLLQRYESQDIIIQSLNEKLKQQSQELEKSRAEGFSLHLQLSQLDVLIKERDQFKQRADSYYQEIQNQYKQIPLLKAQVLDLTRQLKLAEIPVIRISQKTDAIEKLRQQNVELEQKIDDTLEDQIDELQQTNKVLRQELTKQIDINTQLIKDLSNADRKKQDILIELQLLQAIEIDYQQLKIAYLNKQKENDKIKMQSDQIEFKQRDLENAIHSSNQLGLEQKNEIQKLRERTNNQNEQVHELLIKLNSLQQLNNDYKFKLDMFPELQEKVNSQAKQIDDLKKALQNSNEIRDELTAKCTLQQVKLQSLGPLQSKIESLQLILDERQRQLDLWQTKALELEDSKSKLNQEWRKKFDAVEVQLKNQKELDQLLKQQEHDINDLINQNNKLNVQNKELSIANQLLNKDIDQRDIEIKRLSAIETDFRKLNENLLIELQRIADLTDKLQIAQNQYDAAKRDQERLENKCAMMSSEIERLSIMVKNKNIEIEGNKAILHQNQSQIDQVQPIFVENKRLFDELNNVLKSNEELKIQSIQLQKQIDELKSTINNGSNYENQLKDDLDQLEQAFRHKENEIGEIKQLLRQSENQVKDIKRDDQQWIDQQAEKEKLTNQLNYINELLNSKNTENEQLTKQNHVLQSKLLDNQKLDFEHLELALNTRDKVIEEWKGKFQRLSQDLQKIIEDKVELENRFSTVDLEIERWKRKANSNSAEIEMLRVTIDQLNDEIERLNKNTQDLLNENDSWRHKYGNLQQLIPQMVHHQPNPETQAYQVTPGNSFRIPTGNERNQGSFRNQPPPLDSRRSFRRATTIGLTKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YI02</accession>
    <name>A0A8S1YI02_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1810004</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000181">
        <property type="protein sequence ID" value="CAD8213560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YI02"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="153" mass="18378" checksum="53758BD7157FDDAE" modified="2022-10-12" version="1">MRCLIKKTRYLLIIILQEIAEKQQPKTNYLLFLQMQMKLLKKLHYSKFVFDIIFLLNQKLVTIKIRINSIKFSTFNFTNKREIFIKGIKNGAQNCLQELHDYDCYDKPKFSTLKMQLNPLRHLNMKKIGMIILNTQRNSIHKIPPKYINMLMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YLD7</accession>
    <name>A0A8S1YLD7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1970008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000197">
        <property type="protein sequence ID" value="CAD8214131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YLD7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8214131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="162" mass="19231" checksum="B49B6501A99D7808" modified="2022-10-12" version="1">MDKNQIFNSMDKQLQLNKNFIIFADIDVLPDCFAEEQFQLLQLMNSSFKIVYSIQANKPIKSQKQGIYTESKTFLIYTQYSQNISIIALFSSQFKNQHFMLIIFNFIITIKFILNVIQLWLITLITILQERLVHKNTQDVQVTFFILQQNYFNIDIKQSKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YPD3</accession>
    <name>A0A8S1YPD3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1780019</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000178">
        <property type="protein sequence ID" value="CAD8213412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YPD3"/>
    <dbReference type="OrthoDB" id="1068471at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF8">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="24"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="98"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8213412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="198" mass="22709" checksum="925DDE081DF9E983" modified="2022-10-12" version="1">MELHQPLPVKMNQDLNSGEEKIILKEHLGQASSICFTPDGTQLASGNKDALICIWDIKTGEQKTILQGHNDNFISLCFSLMVIGQHPLAKTLLSKYGILDLDDYVRQVCFSSNGAALPFYSEDESIHLWDIESGKEKKQQMDMMELFIHYASLQMASNQPLEVKINQSIYGMLNQEQKIKYQNIKRTQWISFILMIFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RV45</accession>
    <name>A0A8S1RV45_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0010361</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RV45"/>
    <dbReference type="OrthoDB" id="21530at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="4">
        <location>
            <begin position="111"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="143"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="54"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="148"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8131938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="170" mass="20070" checksum="316FEE19B2834CC1" modified="2022-10-12" version="1">MSNAKSDGENPKYMMGDEPVRFQNYVGGQDEQQVPQQDQNLEYYNEYLRLYIANVVLTNQLKELLNEKNELVNKLNKLERRNQELNSNVEETGEERKKRLRRPAQEIERHFTCPIENCQKKYGAEGSLNQHIKLKHPELVKDRAFYKSNEQSQQQGEPESLSDLKQEQNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RWF7</accession>
    <name>A0A8S1RWF7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0010273</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RWF7"/>
    <dbReference type="OrthoDB" id="285047at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24362:SF309">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24362">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8131762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="370" mass="43479" checksum="173D0B5ECCE66AED" modified="2022-10-12" version="1">MYPEYYVGQQKQTQTITKITSSYNPVVQDVPRQRLPNENPKDYMLSAYTSLIHVIDITSTSSIYRVDPFAKYLCCWTNIINQAEISAIQERTKWKHPNLICFLFYQDCSDKFAQTKEFRIYYEYLPLDVKQLIEKRSQNNEYVPEEEIWKMIIGLSDALLFLQKKGISHSALTLESIYFDEEYLLYRVQDVSPFRAKAPHLFSNEFQPPENQTNSKLNRFKHDVFGVGMIILSLALLKDCKDIYNQGVLQCDQLEDNLQKLGKLYPGRVENVLRSMVDLDNEKRPDWIEFQQILQQNKEKQIGLSEQQSNIIQQIDKQKCKRNSDLNNNNQENMEIKINSQKGDGLDQKLINLNKRIQETLSKSFSKNLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S0L9</accession>
    <name>A0A8S1S0L9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0020251</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S0L9"/>
    <dbReference type="OrthoDB" id="3256376at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14014">
        <property type="entry name" value="STKc_PknB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="12"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8133142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="569" mass="66803" checksum="FD8EF55518FD9BD8" modified="2022-10-12" version="1">MEQDQKVEDFVIRKSEFLGKGQYGVVYGCYSEKLKDQKLCAKVLQAQIEKGLIDREIEILEQLNKVDNPNLVKIYRVIKEERQIYIIMERCTGGDLKQLMDQYQLNKKQFTLEQIQEIARQIILGYKSLFDIKVIHRDIKPANILIENGGKGQIIVKLTDFGMGRMLDDITKKQVLTKVGTPAYAAPQLYLEDKYSSKCDVYSLGVILYQLAFNNKLPFSAKTIPELTQFLKALQAKPFQCPELTQEGPQEMKKAIQNLINEMMSYSEEARPGWDGLLKSKLFVNPTKPTNFENQGEMKLLDSIFLYQQQQGQQQYFNEAKFRAACKFANQNKITKTDRINHLVSLFLAKAELSFNLSQSLQEQLLTQFPFFNVYHFELLQTCIIGYRYKILMNAFGFCFNSLDVICEEMRKMYDNQALKQTLLEYQNDHSEWTEQTKENISQFLNQSNKAFIHAENQIIKLMKTNTYPHNIQNFQKLMSIIDNTEFKIYGRWFQFFWKQDLRKLLVYDKSKQYDIGYLKMLVLIEKFLNIDTEFPFEKYSEQDPQQIVLNENLSKQQLEALVQSRKNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S1W4</accession>
    <name>A0A8S1S1W4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tubulin-tyrosine ligase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0020518</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S1W4"/>
    <dbReference type="OrthoDB" id="196367at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_125, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069">
        <property type="entry name" value="TUBULIN TYROSINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8133637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="593" mass="70002" checksum="2104B057E5069C56" modified="2022-10-12" version="1">MLKPDLEFLYIKGNQKFQDVAFQQPIRQHQPKVNAIKLKGFQQFVELINKQQEQQQQLKTCSQCTPQLLPTVISYPPRIEVQTRKASEMKLFVKKSFIDTPTESHSQERSKKDIRLIPQKQFSIKRAITSKNEDSNIIFEQNEFPILIALSNNLSVQPPDRAIYKAFVTKGNNGILIRQSLKQRPWWILIDQPSEDMNFYWTQLRKQAHYEQLQKQQLEMVSFSKLQKKQVNDKLKYKIEDSEYSIFNQSVSLKVHNHLECNFQVCNKKALFYNMKNYYISRNEDPFQFIPLTYHIQTGSNDPAYLEFEQYAQTQNVNLWIVKPGESSNRGNGIQVANSIQKVKSIISQSSNHHNGMKKTFIVQKYMEKPLLYNKRKFDIRCYILMTSINNKFKAYWYQEGYIRTSCKEFSLDDVEDKFTHLTNDAVQKKNQNYGKYETGNKVSFNEFQKYLQDNYNYNFSIIIDQLKKLSTDIVKATYQHLDPNLNFYTFELFGLDYMIDQDMKPWLIEVNTNPCLETCCPLLARLISHLIENTIRIAIDPMYPPPLNKKKHIQDQKNLFELIFSTTLITDTVTQPIIKEDDDEHDDVEDDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S2K3</accession>
    <name>A0A8S1S2K3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0020605</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S2K3"/>
    <dbReference type="OrthoDB" id="10346579at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8133773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="201" mass="24150" checksum="A751B8DB9D7651A2" modified="2022-10-12" version="1">MDLFIEIKWTYETIKFWQKQNEWLCSQTITDHNHYVWGLSQNEQQNRVISCGEDKLILVMEQQNKQWIVIQKITVETEGFRLCFIDNNTFTFQPWQKEYMHVFEINSTNKQYSKTKDIPVDGGGDTSFFPQQYIKYKCILVNKNASNVSLIRKKQNGEFIIEQSIDFGTYHIYGQMTDDGQYLITWDDKSKEYQIRKYQEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S9W4</accession>
    <name>A0A8S1S9W4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">mRNA m(6)A methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.348</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0050475</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>an adenosine in mRNA + S-adenosyl-L-methionine = an N(6)-methyladenosine in mRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55584"/>
            <dbReference type="Rhea" id="RHEA-COMP:12414"/>
            <dbReference type="Rhea" id="RHEA-COMP:12417"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74449"/>
            <dbReference type="EC" id="2.1.1.348"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MT-A70-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.348" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8137046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S9W4"/>
    <dbReference type="OrthoDB" id="10262526at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036396">
        <property type="term" value="C:RNA N6-methyladenosine methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001734">
        <property type="term" value="F:mRNA m(6)A methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000426">
        <property type="entry name" value="Putative N6-adenosine-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007757">
        <property type="entry name" value="MT-A70-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829:SF7">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05063">
        <property type="entry name" value="MT-A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51143">
        <property type="entry name" value="MT_A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="30"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8137046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="317" mass="37256" checksum="732734EF143D6819" modified="2022-10-12" version="1">MQQEEQLKRKSKLVIDNSDEEDIYKKVKLEDLSNSEEEEDIVDVEELEESEEEEKGKKNKKQEIIEDEDYIYVDKLPNSESMLRDLKKQIEQKIKHYKMRYIQEFHENHTKQSFVHNDAIPICTDVRLMDFQKLKDEQLKISGQLFDVIMMDPPWQLSSSQPSRGVAIAYSSLADENISKMPIETLQENGIILIWTINAKYKVTCKLIEQWGYKLIDEIIWCKKTVNGKIAKGHGFYLQHAKENCLVGVKGNINLDLNRFRQGVASDIIFSERRGQSQKPEEIYQYVEQLVPNGYYLEIFGRRNNVRKNWVTIGNEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SFX7</accession>
    <name>A0A8S1SFX7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0070299</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SFX7"/>
    <dbReference type="OrthoDB" id="311714at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053128">
        <property type="entry name" value="Cystatin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12319:SF2">
        <property type="entry name" value="CYSTATIN-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12319">
        <property type="entry name" value="CYSTATIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035887439" evidence="2">
        <location>
            <begin position="16"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="469"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="549"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="551"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8138760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="843" mass="95856" checksum="CC7DF3A59F005013" modified="2022-10-12" version="1">MKLFTLFVLVFLVTANDKVVIPEKMKKILNEIPEIKESIVAMKLLGKDIVLEKVLEIDQVQDESDSIKDSIQFIKVGQTTYNNSGTNYTQPNSTYNYTYTNYSQPTSSYIYNNQSNGSNTTNQTNSTYPYNYYYNTSYPINNGVNNYVTYDNQSSTNNTNSTNITCSTGQFLTSNNTCENCQPNCALCNSYYGCISCYSGYQLYTNGCYLNNTQNSTTNSSDIYDNSTSIDTNSTQYDYNNTSYNNTSYNNTSYNNTSYNNTSYNNTSDNNSTYNTSNNTYYNSTSDNNTQYNNYTNSSRIGQRFNQTRVHLKLRNSFNRFENNNFTQIISYYNQSNHSQNTVVIFNNSEVIQKQSYNEYYLYQNNSLVVLNQEFDPYGNVVYRSINYDFTIVVSKQQGSLIQKQNQEIYGTVVYGYGNFNATYNNYNSESGNGFQNGSQSSYNYSKQSYGSINYDKDYNYASATFNNQSQGQQSSYSGDSFYNGTTQKSNYSNSNNNYNNQNYFSGNKNFNQNYTSYSSSYSNKDSSYQSNDDYLKVDGIGYDNYNTQISNSQSNGYTSSSNYQGDKSVYSSSSSQSNFNSSQDNVVYAQNYTLVNQSTVGYDSAYSSSNQQNDWRNYSNTDYSNGYKSQDSTYIHNDTNAYNVSDTSEYKSDSGYQSQSQNGFEYSNGYNNFASGQAHSVQITNDNTTNTNIDATINQYNSTNQNYNGQTNYGGSSSTKNIDLSVTDNQQYFSQLRGSKKLATGSWKEVNQKEFASSNGQIVEEARQAITKKFNPEQEGFQFDSISSIQEQIVAGINYNIKLRYLNADSKSIIYEVVLYTIPWANQPNQIISCSRFDQTEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SIW3</accession>
    <name>A0A8S1SIW3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0070280</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SIW3"/>
    <dbReference type="OrthoDB" id="436852at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46364">
        <property type="entry name" value="OS08G0421900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01426">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BAH" evidence="6">
        <location>
            <begin position="76"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="5">
        <location>
            <begin position="197"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8138722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="246" mass="29317" checksum="3338046420ABE364" modified="2022-10-12" version="1">MSSPNFNQETDRKLNEIFNSIKLENVKTQEALKNISFTNNLIAQVRHCQSLTLLFQNVQNLSQAHKEFKEIKINNLSIKLNSDVVIYTKEDGHCIAKTTQIIGVVRFKQYTPIIKVQWYYNKKDLKQIIGKYYDCISQRELFLSDQYDYIQPDTIVGEAQVLELKEFEQKNLSNGFIFFCRSFYKNEQIHPPIQNWEKNCKCRQPMNPDRKYVMCDVCQLWYHKECIIFNKNVQKIYVCPQCRKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJF2</accession>
    <name>A0A8S1SJF2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0080123</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJF2"/>
    <dbReference type="OrthoDB" id="406368at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8139487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="307" mass="35369" checksum="A91DDE193CCBE884" modified="2022-10-12" version="1">MRRVQSAAPNLEFSMEDMFKIATKQTSAFGIEGYEVPKKYADPLKQMEERKFSAQKKGQKNLGYVTKRGHYLEDQKKIYEKLPAPNKYDIVKPWVIQAQSTRSRSAPPKRTTFIDQIFKEAKLRGVPGSGKYNIEKPLDEVLKQVEEQKKKKIEASDRPTYLNEIQHLAVINPGPGNYNPRPISKKLKKNETKPSDFITKHQEQAKKRGKSALPDVGSYNPEPLSFTTFNKLQVNTKKKERVDKHTFGTDERFKDPKKSKSKQSLLPGPGQYPLIVQWQGKEQDKSKAKIKNYMQSTTKGIQRSIYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJG3</accession>
    <name>A0A8S1SJG3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">cathepsin L</fullName>
            <ecNumber evidence="10">3.4.22.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPENT_87.1.T0070375</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="11">May be involved in extracellular digestion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Specificity close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity.</text>
            <dbReference type="EC" id="3.4.22.15"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.15" evidence="10"/>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJG3"/>
    <dbReference type="OrthoDB" id="190265at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000104">
        <property type="entry name" value="Cathepsin L 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035887198" description="cathepsin L" evidence="12">
        <location>
            <begin position="24"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="14">
        <location>
            <begin position="32"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="13">
        <location>
            <begin position="113"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8138912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="317" mass="35240" checksum="F783B871E5300D11" modified="2022-10-12" version="1">MSKTLLALGTIALLGTLMMVNQPDQLDFASKYQTFKQKYGKRYSQTEDAYRMAVYTQNVLYAESVNLQEGKRVFGETIFFDLTKEEFAETYLTLKITEEDLNLERVPAKNIQAADKIDWTKNGAVTDVKDQGQCGSCWTFGTTGVLEGFFFTTTGELPNLSEQQLLDCSTFQDFNLGCNGGLPYRALQYVKRSGITTQAAYPYTAVQGSCRIKGGAYHIKGAVQLEATEEALISYLNEGPVSVGVDASNWQYYNPDTEKIFSNCQESLNHAVLAVGYDKDSIKIKNSWSTAWGDKGYIHLARGKNTCGVYNTNVVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SQR3</accession>
    <name>A0A8S1SQR3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AP complex subunit sigma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0120041</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Clathrin-coated vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SQR3"/>
    <dbReference type="OrthoDB" id="371463at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030121">
        <property type="term" value="C:AP-1 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016482">
        <property type="term" value="P:cytosolic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14831">
        <property type="entry name" value="AP1_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000007">
        <property type="entry name" value="AP complex subunit sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044733">
        <property type="entry name" value="AP1_sigma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016635">
        <property type="entry name" value="AP_complex_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000804">
        <property type="entry name" value="Clathrin_sm-chain_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11753">
        <property type="entry name" value="ADAPTOR COMPLEXES SMALL SUBUNIT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015588">
        <property type="entry name" value="AP_complex_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00989">
        <property type="entry name" value="CLAT_ADAPTOR_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="10">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR015588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8143105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="144" mass="17095" checksum="BB81DD1B9A14E9F3" modified="2022-10-12" version="1">MIQCLFLVSRQGKTRLTKWEKQRFLKEINSLVLTRGQKMCNFLEYVEYKIVYKRYASLYFIVICDKEDNELLILEIIHHFVEVLDKYFGNVCELDLIFNFHKAYYILDELLLGGFLQEPSKKIILKAITSQEALIEEGNDDQSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SUM1</accession>
    <name>A0A8S1SUM1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein kinase domain containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0120350</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SUM1"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000726">
        <property type="entry name" value="Calcium-dependent protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000634">
        <property type="entry name" value="Calcium-dependent protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000382">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="34"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="352"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="442"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="478"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8143680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="533" mass="62129" checksum="3DA263FB0278B530" modified="2022-10-12" version="1">MGCLQPKKLNRIVAMQTTNKDLVFTSTADIHKLYSFGKVLGIGAFGKVLVAKMRNNNSKQYAIKMIDKRKVRGREAMLANEIYVLQKLDHPNIIKFYEVYQNELYFYIIMDYCEGGELVERIQKSQKNLSEGQVQNIIFKICSAIMYIHEQGIIHRDIKPENILFSTKDPNAEPKLIDFGLAIKFDSSNLKQLKAAVGTPLYLAPEVIEGKYNEKCDVWSLGILLFHLLCGYPPFYGKNRTDLYENIQYQNLIFDRRHWNNVSDEAKDLIKKMLNKNHNIRPSAKDCLRHSWFRRKFQNPVRLQRGKSTIISMKSPQSEDQRSIYQMLKTFRIGAKFKKEVMKVLVNQMNEKELGRLKQVFQKIDVDNSGTITVQELKEALMEEGSHISHEEIEQLIQLVGFEIVEEEKDDNDCASTTKSSKQLVIKYSDFLAACIDERRVLTREKLWSVFKYFDTQDANFIMKEDIKEALARHGRQLSDEKIDEMIYEIDPNHDNKISFEEFCQMMGVAGVEQTMDFKDEVKEHLIFNQSQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SX05</accession>
    <name>A0A8S1SX05_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Enoyl-CoA hydratase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0110410</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SX05"/>
    <dbReference type="OrthoDB" id="2018133at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000009">
        <property type="entry name" value="Carnitinyl-CoA dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.12.10:FF:000001">
        <property type="entry name" value="Probable enoyl-CoA hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF54">
        <property type="entry name" value="ENOYL-COA HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8142932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="272" mass="30081" checksum="E454A115768577B2" modified="2022-10-12" version="1">MQRIALLHTNFCSYEKVVVERLEQEQIGLIYLNSPNDLNSLSEPMKRDLALAIQELDQDSNIKVLILLSKLEKLFCAGANIKDISKISLESQLKGDIFQNIFQVLESIRKPLIVGINGVALGGGLELALNGDIIVATEECKLGLPELKLGFIPGLGGTQRLTKLIGKTNAMKYILTSDSISAQEAYQRGIVNSVVKKEQLREECINIAKKISEKSLYTLIAAKAAIKNAEEMPISQANKVERQIFNSLLNTKAAKEGVTAFVEKRKPNFRNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T191</accession>
    <name>A0A8S1T191_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF541 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0140253</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T191"/>
    <dbReference type="OrthoDB" id="293665at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052022">
        <property type="entry name" value="26kDa_periplasmic_antigen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007497">
        <property type="entry name" value="SIMPL/DUF541"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34387">
        <property type="entry name" value="SLR1258 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34387:SF2">
        <property type="entry name" value="SLR1258 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04402">
        <property type="entry name" value="SIMPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="160"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8145458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="248" mass="27721" checksum="C9E9BF90A0A8A67B" modified="2022-10-12" version="1">MLILFITLLSIYGKRINKEEKKIEEHVIMNNDDHKGFTTKIPGAGTIDLVPSIGTVSFAIEVMDKDASAALEQANKLVTKAVQSIKYDLLYSNYEIQTGIFQLQIRYDYTTSQQKLIGYTVTNQLYVTTKDMQLIGKIITVGVNAGLNRIDGVTYSNNALEIQKANNEALKLAIEDAKRKATQVANSLNMKFVKILKFKYLDSYGSSVTNGYEPQAGAVQKDERSQPTPIYASESHVRVDIELKVQLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T6V3</accession>
    <name>A0A8S1T6V3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0160040</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8146764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T6V3"/>
    <dbReference type="OrthoDB" id="266718at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06606">
        <property type="entry name" value="STKc_MAPKKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF56">
        <property type="entry name" value="MAPKK KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="47"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8146764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="445" mass="51098" checksum="B616812A5D25E608" modified="2022-10-12" version="1">MNTNNQNIKQQELEQSFIQDVEVIIIDNKEEELERNSLKSDQDIHQWEQGEVLGQGAFGKVVMGLQKNGQIMAVKQVFIQNQNDDKVKQLQKEIEMLSRLQHPNIVRYIGCEQKNQFINIFLEYVSGGSVQTLLERFGCFRERLIKTYLKQILLGLSYLHAKNVIHRDIKGGNILIDNSGRCKLADFGSSKQLSDITHDTVGSICGTPNFMAPEVINQEQYGKKADIWSLGCTIIEMATGQPPYSEYKDAIAVMVRIGKSTQPPPIPSQLSSPEAKDFLSKCLQIDPKQRATADELLKHPFLEEPKSNSLLKKTTSYKFNQRQVQKYHYQNKNSFLLDAENQEQEIQPVSPQFQGQQGQSSTETNLITVKKKKEKPQYQLVIEPEQNDYILTGQETNIAENRNKFQFLEYNEVNLKETYIKPFNNKENQDNLQNELDKILGQYIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T754</accession>
    <name>A0A8S1T754_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cyclin-dependent kinase 2 homolog</fullName>
            <ecNumber evidence="2">2.7.11.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cell division control protein 2 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">cdc2-related kinase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPENT_87.1.T0170049</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">May form a complex composed of at least the catalytic subunit CRK2 and a cyclin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T754"/>
    <dbReference type="OrthoDB" id="288746at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010389">
        <property type="term" value="P:regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07829">
        <property type="entry name" value="STKc_CDK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000611">
        <property type="entry name" value="CMGC family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000737">
        <property type="entry name" value="Protein kinase putative (CRK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF254">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="18"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8147608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="310" mass="35981" checksum="6844196F8703DDC6" modified="2022-10-12" version="1">MQIELDENSLYNNFLQNFTQQTKLGEGTYGIVTKAFDRRRAKFVALKKIKLDNCDDEGVPSTTIREIAILSKLQHPNIISLLEVKYFLDEKKIYLVFDSMKYDLRKYLDKNTSITLNNLKPIIYQILLGLSYCHSRRVLHRDLKPQNILLNETMTVKLADFGLSRVFPFPMPKFTREIATPWYRAPELMLGDDNYGTGVDIWAVGCIMAECLNGFPLFQGDSQVDMLFKIMKLLGTPTDANYFGLSKLPNFMITFPKFRGEDLTQRIPKLENNQLALELLQSMLQLNPGKRPQAKELLRNQWFDDIRNNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TAL3</accession>
    <name>A0A8S1TAL3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">USP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0170076</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="289038at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000022">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF827">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP" evidence="1">
        <location>
            <begin position="1638"/>
            <end position="1970"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8147662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2830" mass="335908" checksum="A420AFE0BDD4D21C" modified="2022-10-12" version="1">MSRELQNMKLLLSEIEKIQTIQTNQQYELIVQALQGVQQYMNETKTSEGDQYLPELVSMSINYIMNHMNYPDQILIMIEKFIKSVLFYINKNIFDDKISIIFCKIMDPNCLIYQNNDLSTNWHDQFMTYSYTKNIEYKQFIDNLKVGSYLDCIRSCQSTNRVNWNRGIVEERNEDFVNVRYLNEQQNSQIYNIKSGYLMPYQTRSQRLDSMFTLQTKQAILATQNDKWILSTILDRIESSYYENIITYTIKFHFQTILDEIKDNQSQEKFQSQNNYHYEQLTACNPRLALCGDHIPRKNNTNEQFVDDSYDILYEQDYLTNKFYAILRPRYSQSLLLIKFINYFGNLGGFEQILGVQQVDILANYMHALGSIHAQLYRYFCQEYIPKLQGLRQIILHSDEQSFRNMGREKINIIIQAYKLLLDRIMKDDQRKEWLQSLGLEFIQICFNCQFLDKQIIGLKLLTDVLKQADQDQSSDYILQWIEKQGILETLFHHSTHLQLLERTKELIKIYLTIDGAFNLKHLQIIWNLMQSNDKEVRSIIFKLFNDIATFMSDYQVNYFLYQIKLLRKDLILIEHVQLLCQMNKNRINQQSLLLSQSIEILWDIIEYAGQQGKTKLEDQSKTQLIELIKFLNDKDIKLQMIQKLINNIKQQKVECSSLKVFQQIISTLLPQQKIQQFNNRYQLSQVDNNNIQQQQNNNNNIINNKQENDLDNEFMKGLIQQQKVHHTLRDSINNLYIQNLNINADDVNETTLYINKLQERLLLINTLIMIYGTAADMIDQQFLDQIWNKHILKPVCKQESEIVSNWLRDLIEKDERFSSIQLNDFYQRFLKSDSHLNENAFKCFKLILYYVNQKDYRIERQQLILNQLCDDYGNLQSMANANAQEKDEQMDFKIMVDVNEIIGIKYLWHIVFNSQLDSAIQLLVKLNLSQQEQFIQQILQKMKTQDDQIILRCLDILRELLNQTEKYGIGQQLSLDGLIKEEQFNINIIKDMGDVQQSNNITLTVYQNLTIFELRKLIAKEIKTSWEQIKLHRNNIIILDTDNGKTLKQLNIKRSDYIKVSRRNTKPIPKVPLIENNKLSPMFLKVIQNLFNQYSTDGRMNIDQFTQYGIQASKNENIKQGEKIHQIFSQFSTQGEFLTLNNFIAFFEDCCLQQQKIQLVWQNLYAFGYRNDLSLINSSDFTLDELKLPRYYLIQNQEFYQLMCNFMTETNKIAQECWKLLCSLPIFVAAKQRLINFEDFSQDTSYQLIYSLKIIQYLLQDQEYCRYFIIRQGFKQLQLILNNLQHQNSVIRKLGQLIILNIFIKHISAQFRSQYNNIFEIQKHVKQPLSVSLENINQLIEGKCQEQDNKAWQRVQETEEFLELVKQTSDLDIKLDYTNLFMYLINIPIDINEDKQLIEYVLILITTLVGFNLDNCTYLFIQEINSIKQRYQQLLFAPKQLLIRKFTINSLYLIFRCQKTKLNNIVILMLKDLFPSNENKDSQCYFDLLAKLILEQKYSDQELANYIIEKLQNYQSNESRITLSADKTLIGLLLILESLCIVDSSILTPQLIKYLYTVHLFCFNLIQFQMNPNQQSTQNYVKSKSPESRKIVYRIIIQYLKQNFNLEIIDLQTVLKQIRLYEGFEIKLIPKSNHGFVGIRNLGCICFMNAMMQQFFMTPEFRYCMLRAEDGLEEKIIFHKDNQGFEYQIDDNPFHQFQKMLASLDLSDRADFNPYQFCLTYKDYENQPVNVSMQQDAQEFLNQFFDRLDNQLKGKGWQQFIEAIYGGYICSQMICNGCQTMREKFDLFYTLSLKVKDVKSLYESFESMIQGEIIKDYKCEQCQQKNDLIKRQCLSKLPNVLIIHLQRIVFNLDLYMNEKLSSLLEFPHLLNLSKYTREALSDFEFKQDSYYQYKLKGVVVHQGSAQCGHYYSLINTNDDRWLKFNDSVIEEFDLNLLSNECFGGKQSELNQGFQVVGSTNAYILVYQRIQKETVELKFSNEEQKQEIMSKFDCEQEQNSNLIVKMDYQTMAKQFISDVLYGEIQHDNHRFMMERHIYSDEFFRFVKEIAEVYPFPRQYNLIKNADQLPQYFGIYSNYQQVKNVEQIQILLFNLTYIGIELLSRSSDHGSIKDYVLVIINLINIIPTQLFPIFQQLFIQNMQRVFNVLLCAPDQQVRQAIQQIMLHYLNVIIYIHAFTLNTSLLFQLEQDKSEDQFERLIVQFIVCLLQKLQNDVPKNASKFTQYFDFWKLFVKSSQINIFFANQIELVSILADFFMEKQSPKGLENNQCYEYLYDQKIGINNLNVIPFYSSLFQCLDYLLQNQNYELTKNDKICLESKIFYKKALKECQDFDTIESIILKRVFNNLALSREIIEIVLEEIQISNYENVGQFLKLTESLLNIYDNIQQERIQWILGYPQPNYLDNYQTGCQTNLSQQCISYLTHIGCQDETISILNQLFFNRRNYLNLAILMMKMLLTVMNQNKPVFSYIINLPPPSYLYSKYIDWFETFLNEFKEDCIKYPAITDSILFNKQAEFKQTIKLWEIFQINFKQITKLRLYDPIQPNYIIGKIMETGSVSEETQIDNVFLETHESLVYYIESNPNLQSNKTFPYTILKDNIYIQSSKIESSSNIFLIINNDENVQRSTIQYEIDENIYSNETGYNEQQQVNDDFINELQNNQFKNQNILRQRQQINQFQEDYIQQQQNIECNLQIQVDQLLKRFMINNQTNQTIHISYQFTQDPDSQINFILPKNVCKVVPPYSVKYLTTIMKTIPLIEWGNFYSNLNININNTNNNDQQQQIKQQVQTIVDIQTTDIKNQTKIEEEIQCPQCTILQSSSNHFCELCQFSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TF78</accession>
    <name>A0A8S1TF78_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0190186</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TF78"/>
    <dbReference type="OrthoDB" id="301221at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8149592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="271" mass="31543" checksum="1C7EED07ADA1CAB6" modified="2022-10-12" version="1">MQINYYFNILQQNSQIFQGAQIQTQKKKIQLQQLSMPGRQCLPKRNKKYQKLTNEERCFIMNMKQQGMGCQEISKRLNKNLKTVQSVQSSERKSEYKSLKAAITQYGLDQLLQITSIQSMNDRKLKKLATKIIKNVMPPKNKTKFAHLIPAQKTDSNKRRIIDQIVNNILDNIQKILIIRNMTSLLEPNNSSQGDTMCLDNSEFSKFSSNIIHDESEALDNQYNEGIPNNEYYSDNVPEIFQQFEGQSINFLNRSVFEKTIEESSDCYFDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TH61</accession>
    <name>A0A8S1TH61_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0210185</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TH61"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8151127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="257" mass="30928" checksum="A4FE98E45131995F" modified="2022-10-12" version="1">MQYIQQLDLSRYIPGGEALKTPCNQNKADKVKLWELAKLRCVSYCFQFTGRMTREQRFNIFCTFFRSKLYVLHSKLASDMHYRNFIKEMFDTTISIVLLMVRYECGDELYQEMRRYFRILKDAIDYLVTHYNVEQVTTIITSEDFHKIIFYLKLLNLMRNIKQRKKFLEHYPHSAIKAIQQCYLYNVIINGPVKHIKYRFAVANQDYIKYGLQFNIESSNKFVNKQRVAEICWQTLYDLNIATTIKEGIWDDLKLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TIH4</accession>
    <name>A0A8S1TIH4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EFHB C-terminal EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0200405</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TIH4"/>
    <dbReference type="OrthoDB" id="2096280at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057428">
        <property type="entry name" value="EFHB_EF-hand_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040193">
        <property type="entry name" value="EFHC1/EFHC2/EFHB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12086">
        <property type="entry name" value="EF-HAND DOMAIN C-TERMINAL CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12086:SF12">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING FAMILY MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25325">
        <property type="entry name" value="EF-hand_EFHB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EFHB C-terminal EF-hand" evidence="5">
        <location>
            <begin position="340"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8150679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="416" mass="47802" checksum="D19C14D54EF703B7" modified="2022-10-12" version="1">MSGKTRDYFGTLKSAGRTVLKEDSAGACIQPIQNQVLETPPHIKKYRKSYKHQHGCSILHPGLVDAPKQQGNWIYGRKTEFSDKAGELFKQQPQGIKELINEINEQKYASHIKEPLGTMPTRNYNWPDEAKSDGFAFGQKIPPSEYSAKEVVFPPDAKRDEERIRLMYLKSHGNFEAGEQKNREYNWKINPNDYRFGKKEEREQEQMKKILQHELTQNQYPKTTIISKNQEDWKNYNEDPLGKPKNQAQLNPRMPQIFGEMKKNEQWTAGQCINGQPTQKEVQHDLDLGKATKFGFRNQPKPGDETRAFGVPAIRNDINKKGMKSVADPQNYGDEVPAVALLFPEKFSHMGLSEQDFLCLRTKKEIKDIFESIGIKYGIGKFEGIFKRAIEIQSAQDDKVSVKAFQLAVQEMHHID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TKH9</accession>
    <name>A0A8S1TKH9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">RCK N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T0230247</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TKH9"/>
    <dbReference type="OrthoDB" id="10035564at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005267">
        <property type="term" value="F:potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000146">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000797">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003929">
        <property type="entry name" value="K_chnl_BK_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047871">
        <property type="entry name" value="K_chnl_Slo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027:SF10">
        <property type="entry name" value="SLOWPOKE 2, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03493">
        <property type="entry name" value="BK_channel_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22614">
        <property type="entry name" value="Slo-like_RCK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="204"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="228"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="13">
        <location>
            <begin position="626"/>
            <end position="767"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8152800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="982" mass="112747" checksum="B0FA21119F4674FC" modified="2022-10-12" version="1">MIYEGKQSIFSHQDQYALIRMVNSEDDATTKKHEEGKTTFTLKMAKYLSTSGIGDYLDGINVLLSFLLTVLHLIDCSFWVQGGNQDVGEGQAIITIPELICYIYFLLDFVINFYLSENKLFFTFQTTSLIEYVSIFPSLLARLNIITGYKYIYMLRVLRFLLCYKLDKVLQRLSMEAYKPIVTVMAIIMINSSVLYVVEQDYSIVEYIYFMVVTISTVGFGDVYPTTIYGRFSIIVAILIMFLVLPTQVEMLTRVYSLRSQYARNKYISKKESEHVLLLGSSQVEGFKTFLNELYHSDHGMNDTNTVILQPCAPTEEMTLQLKQPALQSKVIYLEGHPLQNKDLERCSSKDCNCVIVLANKTSRTPKRDDYRNIIHAFAVKQFAKKQKSRKGARVCLQVLQPSSKDLYFNSLGGHETDQVICVDELKLYLLGKTCLCPGINTLISFLIQSSKPSYDITKYDKNKGEWIDDYLCGMQNEIYRVPLESVAFVGLTFSQISSAIYKELNIILFALEVELESGTSVFVNPVEYLFEDYLHYGYVIANEMPNIDEIQQTKFPEYIQKNYCFPNQQRQQINKNALQQEAEYLKEILSEGIKDDSSKHPAYYQVKPQTITTGLPKVGDQEKFENHFIVCGVVTDMKYLMMPLRARSLKNIQPIVILNQDLIPTEIQLQINKFPKVYFQQGSPLNTEDLKKACIQKASALVILQKSADQEEGLSNIVDADTIFIYKTVKLLNQNINIITELASISTISFLQISRNNYVQKYDWSVSEPFASGEIYISTMLDTLICQAYYNPFITRSASVNKKHKKLYQATKLQQSNLFLINIPPKYQEKTFGELFEILLTEQKMIPIGLYRGEKVKNNNKPYVFLKPPMDVVVSSKDRLYVLSGKQPKEQENILDNEKNQGKETASQYYNIKSKLQGEEGRVDIEFSRELMKFKDKMQSFVKGLNKINSEVFNQGLIHEEFISKVRGTLKEELANLNPAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TKN7</accession>
    <name>A0A8S1TKN7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0240257</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TKN7"/>
    <dbReference type="OrthoDB" id="299729at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8153505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="829" mass="98547" checksum="9C0567854EC1386C" modified="2022-10-12" version="1">MDNQYQIAKNLLQSILIIKQQDSNLDSAYSSILLALQSQLPLKQEHYTSILIEITKKLTKQIEQYDEYYTSQMLKILQNIPKQQYIDQLQCTISILSTLINLLAKVNQTFIPEIFQLISLLIDNSKALQIAYPQLFIKTLTCLLKYSRGVIQENGLLLFEKILSYIVCNTQSQYQQLNFNVISLKYEENQHFLVQQKVNEELQNFCLFITHLPTPKLNKCYSQLIFKIFQYSNSGQYFYIPTQISLINLYFRFNEGQNIQEFTNLIIQMNSQQELRIYNELEEDLEFELKRFHSLQNEVDTSQTIAIIRKIKWLIYIIIEFQKKQQNQLQYVQISEKIIKYLVQGLQYKWNQIVVSVDQQITTLNQNLNISIKNTINKLNLELMPILNNEQSILNEIISLLNSLNENEIINKYLENKLEKYLNKLIQTIIVDDSQIIKIQITQILYTSSILFILNNISSTQKHDSYYFKNLIKQFSMLIFNQNAPYNSIFQSQLIIYSNLIYQRIDEKDDQFIEVLYLDYLMCQNSQSTVLITEISQNYIKQFNIYDHIASILYRNIQKIQESNLQVDQGNSLQTILHIYSKFDGYNQGLDIIYQVLKTIYQIYDKYYLKSDILFTQKYLQFLILFFKQWINPSLNNNIVTILRQIALSLTQFFHHSQVHFQAFQCFYYCIPILAKQELQECNSKQVFGMNDDPNVKVVDTLGTIFFEQRKSFEYILTQDNQISKLYIWMIIKELLIYDSCQGFFQLKSIQQFLKQYLVFEQEENSNSLKIYEQILQFLILYCKKHEQNPNLMDLLKQIEFFIKKCSKLDASQLKELCNHLNQKLSLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TN98</accession>
    <name>A0A8S1TN98_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0230218</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TN98"/>
    <dbReference type="OrthoDB" id="354826at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000454">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000819">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF374">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="23"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="10">
        <location>
            <begin position="290"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8152742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="354" mass="41091" checksum="04DFA2EEB9CEABDA" modified="2022-10-12" version="1">MGNCNVLAKQEEFQESMQSIRNYEIIEVLGKGGFGKVLKVRRKKNNQLFAIKKMSKAKIITEKCITAILNEKNLLIQLRHPFIVNMHSAFQDKESLYLVLDLLTGGDLRFHIYKNKRFSEQEVSMKNCSIIEFFAACIITSLDYLHQQGIIHRDLKPENLVFDDKGYLRLTDMGIARIWRPQNSSDASGTPGYMAPEVLCRQNHGIAVDFFALGIILHECMLGKRPYVGKTRQEIRDQILAHQAQIKRMDLPVAWSLEAADFVNQLIQRKPQNRLGTNNPNDVKNHPWFSQFDWNSLLKKTLPPPYKPKPYSKQNNFYDSTETPEDDNFVILRNNTLNEQFNQYCYNPEICMQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TP16</accession>
    <name>A0A8S1TP16_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0240159</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TP16"/>
    <dbReference type="OrthoDB" id="300198at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="271"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="494"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="495"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8153347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="617" mass="71325" checksum="B873D377E22BF553" modified="2022-10-12" version="1">MSASVGDNVSEREVIEFSEDDHLSFFYNIDQIYTELPGVISNYGTFIQSLLICRQLSNQIIKNSISEAQERFIQRNIPINTINELQAQTNLILNSNLNDGGIEVETCQLEEDQEPEPIFSDRWRRNRVLMMAEVETQDKIDIEDQLNNDKNKSISKSKLFDTRKNTVRLPTTPPPPNQFEAKLIYVYQEEFEDEYEDKLRNAKLYEMKKKNEIEQKKKEDKLQSQIEKKDGAIQKYTYDFDGKIILARAIKMDKLQPTNQKLKVELKEQTKQEQQRNVKKIGRRDSTKVNKPQCPEQDIPNRVIQERRDAAKEVIGDKALRVDKTSQFPYEVFTMNNGVKLYFEQKMKEGVRHQISDSAENLQIKLSGSNLLSGEDASQFASQIRLTKAEYQLITDNQILQQARQQFMPSENINIPDHDQQVTQQSILLKKNKAILNNTNHPTESKQVQFNAGISQLNPYKRDSPIQQYAQTERIQLKNVKMIENLLVTGVPDTPKSSKVDQPQIQIPNNTSNNPIDLFNQQLLTQKEQGKQGGGKTAYFPPVKLSKRSLETKAYKGSLEQLYKMPRERLVAQTGRTATNFYKQNSDGTKKNSKSVSDGFMQTFYTTHTKFSERLRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TPS8</accession>
    <name>A0A8S1TPS8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0250156</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8154038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TPS8"/>
    <dbReference type="OrthoDB" id="291316at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398:SF0">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="423"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="423"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8154038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="623" mass="72756" checksum="F360498EE83A8374" modified="2022-10-12" version="1">MNHNSMKKLRNLGRKLDFFQSPVELMIHKKKSHQSLFGAFMTILMFSFVLSYIINKFVQLGQRKEFQTLYSEVYYEEIPIFPLNSKNFSLQFAFQTENQTNYIDETIYQVNAVMINKAQVIVNQKSSLQLTRTVIPLSKCTKIGIPYEDLEDQLDNVDSENTYCLDWNRVSDLSLIGTPEQENYTYIYISFQQCVNDTINIKSPVCKSKEEIQEKLHRNFILFQLSSYNIDLRNYLKPNVPKVEEIQTTISSKVKKDITLFMQPITTLTEEGLLDELVRKDSTIRYMKSQEVIDFNSDEALASVLIRLANTENISYRIYPKLQDILAQAGGLWEVLMLVFTIMVKPFQALSYKLDVINNLFNFEGQKSQNDQEDGSKQIIKRLTIVQEGVHNNEQDTSNILNQQQIKSSIRFPRGRLKTRTSKLLNSNSAQTEKSSNQMTNSPENDKLIQKGIRYALRKLFNIATLKLRFSPFDYLKYLKWGKKEGKFKQFNYSINKLEKCLDILFIIDKLQEIDKLKMILLTKQQVQLFDFLPKPLISLDPSNLQYNEQTMYFSLLKPYKSQKEKAHEAQLVLDELLENLDDPITIKLISLMDPNIFKLLQIQLDFKKRKLSKVITPDIIES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TU41</accession>
    <name>A0A8S1TU41_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0270074</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TU41"/>
    <dbReference type="OrthoDB" id="283035at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46364">
        <property type="entry name" value="OS08G0421900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BAH" evidence="6">
        <location>
            <begin position="41"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="5">
        <location>
            <begin position="171"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8155404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="26012" checksum="5436ABCCC35F4DD4" modified="2022-10-12" version="1">MKEDNSILSQKKIDELIKEQKYSTLIQLISKKEPCKLKQYNSIKIKNQIFRLKQDVAVCANNNDVYSGKLIKIYSFKDQNEQHVPVIQIQWYYTKQDLNLDKKFMKYISIKELFFSTHVEFLAANKLQSPIEVMSFDQYTQLEYVEETKFFSRAAIDLKTMEPMPKVTEWPKSCVCRMPQNPDIQMIQCETCGEWFHLDCVNIKSEEAEQIENYKCPGCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U886</accession>
    <name>A0A8S1U886_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0350308</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8161060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U886"/>
    <dbReference type="OrthoDB" id="297939at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="547"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="216"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="58"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="553"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8161060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="628" mass="73530" checksum="A85EBFC010713329" modified="2022-10-12" version="1">MKINQPKFKMRKPIGLTLDFLDIQQSQKEQTPKVQQIDTFEEFCSQIPENQQQQELNFSKQSQSFHSQQIKSNNQEESICSEFKQSIINQEKENSQIQYIVFEEFHPQSTIISGQSESIRNSEKQLQEKNTDLIKEPPAPSNKNNTKFSFPQKGTGFTGLKKQLQIAIPEADHTNTSSYEQTMIKQQLKQNQMNNAADMELKQQIFTLQRDKEQIKNQYKLEIIDLNAKIQELQQKLDIEQANTLQLKQENEKLQLKIQQIEHQYSINIDKNQSDQFSISYQNKLSDNEKIQYLVEKLNQKSAINAKLQYEIFKLQNNIELLQTKQIVQSYNINQISDYEQISDSHSPPQFASNPPLNNYKNRESNKASVLMQQKQALLNLSVNQAQLDQKKQINLSNMISPQAKTCKASTTIHKNLTTHQTKNTQFKEDTLQHLRKMQSTKNSTSVGELNTFKQDLTNCSNILQKIMRKDSTQSPDKPIQATQKFKEALNSKLQNVPDKHKSYASLLFSLGDFNKPRIQDAYIRQNSPHINEDIQFKSRQIFAYQPHQNSRKQSETIKSEESHKMSTEQPKYYDKYEYSNYQRKDILQLMRDIKSQKLGISAKINYKKNHPKYEKQYNLCQYKGTYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U8K6</accession>
    <name>A0A8S1U8K6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Acyl-CoA dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0350145</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U8K6"/>
    <dbReference type="OrthoDB" id="435240at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000013">
        <property type="entry name" value="Acyl-coenzyme A oxidase 4 peroxisomal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000021">
        <property type="entry name" value="Acyl-coenzyme A oxidase 4, peroxisomal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045008">
        <property type="entry name" value="ACX4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43188:SF1">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43188">
        <property type="entry name" value="ACYL-COENZYME A OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="4">
        <location>
            <begin position="31"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="3">
        <location>
            <begin position="148"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="2">
        <location>
            <begin position="258"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8160734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="404" mass="45404" checksum="643B3F714EC15E6B" modified="2022-10-12" version="1">MIRLNQLAGQVKEKKAQIDPMEYLSLYDLLTPEELEVRKAVEAFVAKEVTPKINDYVEAAQFPTEIVNAVKPLKLFHHFLNKPYGHGTSYTKQGIILMEAARADAGFATFIAVQNLLLNYTLEKFGSEAQKQKYLTRTRDIEYIGGWGLTEKGYGSDASSLTSNVKKVQGGYILNGDKRWIGNGNKDVLIVWARNLDTNKVEGFIVETKWPGYHAEVIQGKLALRIVQNCQITFTNIFIPDENKLESVTDFQNGPNQVLMHSRVIVPWIALGVMRGVYEHSLKWTTTRKQFGKYLASFQLQQERLVRILSTFQASFLMVLRLSRLAAEGKATIGMISSVKAWVTDKTREVARLGREMLGGDGIISDNYVIKALTDAEVLYTYEGTYDINSLVAGREITGIGAFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UC40</accession>
    <name>A0A8S1UC40_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0360077</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UC40"/>
    <dbReference type="OrthoDB" id="17199at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8161252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="86" mass="10381" checksum="216BC7B7CEDC8073" modified="2022-10-12" version="1">MIYWLYKYLFLLKSTNQMKSIQKYWIKMKLIQLKVTLQQKSNQKRKFSDGITREELGQTGWTLLHMISATLSVDLRRNSNLKSMFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UCF0</accession>
    <name>A0A8S1UCF0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribosomal RNA small subunit methyltransferase H</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0360167</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UCF0"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071424">
        <property type="term" value="F:rRNA (cytosine-N4-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002903">
        <property type="entry name" value="RsmH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00006">
        <property type="entry name" value="16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11265:SF0">
        <property type="entry name" value="12S RRNA N4-METHYLCYTIDINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11265">
        <property type="entry name" value="S-ADENOSYL-METHYLTRANSFERASE MRAW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01795">
        <property type="entry name" value="Methyltransf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004486">
        <property type="entry name" value="MraW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8161399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="277" mass="32376" checksum="9772D3F4651407D4" modified="2022-10-12" version="1">MHKEILERVLAFQKKTLKNNIDMVDCTIGTGAHSSILLENVKNLKILGLDIDERMIKNLPQNIIAKQDNFINFYKHKTLSDTKYFDIIFADLGYNINQVYDESYGLSYKKNSVLDMRYSQSIQYTAADLLNNRSQQELREIFHNYGNIHKANQLAKIIIDNRQKEKFERANQIIDILNELSMGDQIMKTFQSLRIAVNQEINNLNLFLEKVQSQQIVDKQLILIITFHSLEAEAVQKFIMKFKKQFSIKIIKPSEDEIKENPRSRSAQLFEIINKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UGJ6</accession>
    <name>A0A8S1UGJ6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="27">Calcium-dependent protein kinase 1</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="33" type="ORF">PPENT_87.1.T0380293</name>
    </gene>
    <organism evidence="33 34">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="33" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cell membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="26">Parasitophorous vacuole membrane</location>
            <topology evidence="26">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="23">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="33">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UGJ6"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020002">
        <property type="term" value="C:host cell plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020005">
        <property type="term" value="C:symbiont-containing vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000398">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000315">
        <property type="entry name" value="Calcium-dependent protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000557">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16 28 29" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="21" id="KW-0966">Cell projection</keyword>
    <keyword evidence="19" id="KW-0969">Cilium</keyword>
    <keyword evidence="17" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1032">Host cell membrane</keyword>
    <keyword evidence="18" id="KW-1043">Host membrane</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="22" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0519">Myristate</keyword>
    <keyword evidence="13 28 29" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-0564">Palmitate</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 29" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="31">
        <location>
            <begin position="45"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="32">
        <location>
            <begin position="342"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="32">
        <location>
            <begin position="379"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="32">
        <location>
            <begin position="415"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="32">
        <location>
            <begin position="453"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="30">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="30">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00060437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00068067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="EMBL" id="CAD8162889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="482" mass="55621" checksum="FAC3BBB23F38408A" modified="2022-10-12" version="1">MGCASSDSASTDNPRKREPQKEFTGEKISSEIFVDLKDGNIYEEYEVRSTLGEGAFGCVKLVAHRKTKMPYAMKQMKKQGLIKEDQKILFSEMDILKLIDHPNIVKLHKLYQDNIHYYLITELCQGGELFDKLASEKNFTEKKAAEIMKQVLSAVTYCHERKIIHRDLKLENILLETKSANSNIKVIDFGTSRRVQEDEKLKLKIGTLYYMAPEVFQGQYDLKVDVWSVGVILYILLCGYPPFNGDDTTIKKKIQKGTFEFNDTEWRSISQEAKDLITKMLKHDPQQRITAQQALQDPWIQSKAPNNPVQVNALNNLKDFYSTSKLKNAIQLFIVTQVTTYQEKEEQLKQFKAMDTDGNGTISPEELKKHYSKFYGQEQAEKLVQEIMKQVDINQSGQIDFNEFLVAAANKEKLLSQEKLKQVFQMFDKNGDGKIQRAELQYIMSGIKIDEIQWKNILEECDKDKDGEISLDELITLMQKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UH87</accession>
    <name>A0A8S1UH87_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0390042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UH87"/>
    <dbReference type="OrthoDB" id="310402at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8163099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="195" mass="23531" checksum="FCF29676D3D7E01E" modified="2022-10-12" version="1">MDQPRHIFKRVKERSIMDAKQSVDEWRKLFKNGEIDQNGHKIKYSLKQAAEKVGVPKKTLEDYHQLLKKAEQYIDLNEVGDKKMGYLRSFLKLQQQQQQMQSEIFSDEEKNINTEYMEEENEVQQQQQQQEQQFDFEDMIVDGDIYNYNMDEQFEFVQQHVQTVLIDHQLNYDKKLDFAYSDCDDCETDDEFDNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UHD9</accession>
    <name>A0A8S1UHD9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0400057</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8163774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UHD9"/>
    <dbReference type="OrthoDB" id="298946at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8163774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="202" mass="22869" checksum="9A743C183567B84D" modified="2022-10-12" version="1">MEVNMKELNEGPALVIDGQTNVDLDSEDRNLQEGQGTNPPQVPTKPVKYVLTNSDKMTLKLAKGLILCNLIGFVFLFIGSAIIYTTLKSNWYDEMIVSFQIFIFSDALLLGEKILFFASLINKKKIVKLCPFLNVVSLLLKIVASGLIVHTLLCALIVSLILVLLQMPHNIMIKVITKRWRNIPDRKWKKQLILVRKPIPQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UIP0</accession>
    <name>A0A8S1UIP0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0400284</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8164224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UIP0"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00157">
        <property type="entry name" value="Rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000983">
        <property type="entry name" value="Rho family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="178"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8164224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="194" mass="21806" checksum="84C256C9065FA924" modified="2022-10-12" version="1">MSKSLGTPIKLVVIGDGTVGKTCALLTYTTGKFPEDYIPTIFENYSASITIDGKKVNLGLWDTAGQEDFKQIRPLSYPNTDVFLLFFAVNEQSSYINAQQKWYPELRSALNNVPIIIVGSKIDLRQNDNKCVQRESAKRMADQLGCLYLECSAKDKIGLNELFEEAVRTALRSKKPNRAQEESKQNKDKSCQLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UJ00</accession>
    <name>A0A8S1UJ00_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0400024</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8163722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UJ00"/>
    <dbReference type="OrthoDB" id="291910at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8163722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="99" mass="11414" checksum="BEE1320E1C78A05E" modified="2022-10-12" version="1">MNDTIAKEITPTDISDTEKRIIQYLEEELLLDQFTDPQLKQINFELSKKKTIFSFKSMDSDTEISEATSCSTIKSTQLQMCKSPLFRRRLSTLVLDQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UKD7</accession>
    <name>A0A8S1UKD7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0410118</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UKD7"/>
    <dbReference type="OrthoDB" id="289431at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05380">
        <property type="entry name" value="CAP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035746155" description="SCP domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="3">
        <location>
            <begin position="34"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8164607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="247" mass="28842" checksum="8EDBE8CDE5DFE5EC" modified="2022-10-12" version="1">MKFFLIILFTIGLAINECEVDNKDRVKLSGMSKQKEDELLELHNEHRNLVAIGKVRNYYGEFSTATNMNCIKWDDHLAKNAQDCADKCPSNFKLDCSFPRHYGYLTYKGEVDNGGSDWSATRIFKKLISQEDFARQIELATVQFFGCGRTQISRRNGKSQEIFVCVYNKQSNLNGEVYKYGIPGQDCLYGRKIEFQGLCKQSITDIESLKFRHQRHQDAYKYHHKDEKHHVHHNHHRKDDGFHTRAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UT55</accession>
    <name>A0A8S1UT55_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0480068</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000048">
        <property type="protein sequence ID" value="CAD8167881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UT55"/>
    <dbReference type="OrthoDB" id="163438at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="329"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="392"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="123"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="190"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="223"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8167881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="473" mass="54817" checksum="4737650180D9CF94" modified="2022-10-12" version="1">MQFQSRDPINYINREINLYGLSQTTFFKDQSSNTQRIYVELYSKVRNGDAKGVQYILGIKDDDSLINVQPINIQQIVDEKQNTLLMHAVWKDNTQIFIDLIKYTLQNFGKSETQQWVTQQNDSGVQCMHLATQKGNIEILDTILKLGIQLDQKTKLGQTTLHIASQSNQPILLYYLIKIAKVNIDITDNDNSTALHLASDQGSEQCASLLLAWGCKINQKNINGCSPLHVAARGGEIGITKKLLFYNAKTRLRNKQGKTAYQLSIENEYYDISQMILEYQEGNIKCNNLFGQKNKARINKKCRFQMYTFILMLILNLIYQILFTIGNNLFGYIQIISDIFELFLFIYIVNSDPGYQIQYKQEGQLFYQILQNNPQNLEICSECETLKAKRSVLLYLIIIVHGQIIVLVLKIILYSQYLYGSLSQQLVYNYLFQLYCLSILYGNKHLLKQPPQNFIICKHLIYYLIHSMIQEYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UWN3</accession>
    <name>A0A8S1UWN3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0500143</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8169065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UWN3"/>
    <dbReference type="OrthoDB" id="307921at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8169065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="359" mass="42458" checksum="7C8FD8B62FD8D199" modified="2022-10-12" version="1">MESCQNEKHIGNLLFGYCSNMNCNNNLKTFCDKCRCYHDEHLQDLHQLDSLDNIKLKNIEYNNQLLNFFENQKRFCLEIQTPLMLINQNNNLKIDYQNKEVLIDQIKQIVTIQSILNAVLPMFQNINKSCTQIIQEFNKLQQFNYNLDIQKQEIKEKKNILKKQKIVRKQDQKSKLLSEKQNITEIIQWQNLQIHFSQKFKYKWIKILNNGLIAQNEDDRGMVICEPMLPKKGQYRFAFGINDNAYLIWIGICHKEYLIQNNYKTNFGIQSDNKNGVYLINNYGVTNSHLNPDLNDQTNSFFFELDDLIIVYVDMDIGNIKWESKKSNNQYSMQFDTSQDVYPCAGITAFSPIFIVQEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UXB4</accession>
    <name>A0A8S1UXB4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0490148</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UXB4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="13"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8168502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="189" mass="22686" checksum="7CF27B4A6698B62C" modified="2022-10-12" version="1">MNQNQQWNQCYDYKKLKAQVIELKRQIDSLSQSKPIYIQAEVEGFLREYQMENEKLRELLKQQIEKNESLQEELKIVNSSYLQNTKRFQLHIQQLEQRFQEILINCHNYSENIDNFEQSTLQDKQKLQDLEDQEIQRKTELQKLFKGKIIKNISEINNGQQNTTSINSSQGQQQINQQTNEIYELLDND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UXB5</accession>
    <name>A0A8S1UXB5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0510014</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000051">
        <property type="protein sequence ID" value="CAD8169371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UXB5"/>
    <dbReference type="OrthoDB" id="4044674at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF12">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="263"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="332"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="358"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="403"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="426"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="17"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8169371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="476" mass="53835" checksum="D2ACF4643F07A833" modified="2022-10-12" version="1">MKTNISENSPKVNSVFQVVLMNVNIQLGALNIGYVLSYLTLSIDTLFDNLGITKEEKTSSLSLINGILPLGCVVGVIIGYFLKRKFTNKQCIHIADLIGLSSLLAVIANTNVVIVFRFFLGVANGISSLIMPVYVKSLCPEKYYYQISMILGYGVNTGLAIGQLMGIGYIKYHGVTSNWWRIVFLFPAIIFIIRSTVIYFFYNYDSPEQSLQRNDIEKAKYVIMKIYKEEYVEGQLDRYRQIAQNEQFKTQNSFLHIFQKKNLIGLQVGVISMFVQIWCGSFAVFYYSAQIFEQLSGGDLVLKTIYTICIGLFSAVAQFTTIPMLPRLGQKYILIIGSILMGAINITIAILSNYQSDTILFLIFVFLVLLIMVFAATLSPVCWGIVPQLNDSDGTFFTMDLRWIFQAILIFSFPYIVQGVNISGAFYIFGGIDLFYTIYCGFFILDSRGKTGTQILEMYFQKYSKFLVLPQTETNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V2D9</accession>
    <name>A0A8S1V2D9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0500065</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8168936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V2D9"/>
    <dbReference type="OrthoDB" id="308205at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035724326" evidence="3">
        <location>
            <begin position="17"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="202"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="77"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8168936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="518" mass="61383" checksum="A8885217F9656B23" modified="2022-10-12" version="1">MLYILALMQLFSLVVTEDPVDKLFEFQQLMKQKTKVPKQKISEYDSSDIFSEGYITVSDQSEDDEYQQYDEIQQYDEEVDETDDESDDEEMIIQQQKEQQKIEQQKIEQQKIEQQRLEQLKLEQQRQEQLRLEQQLIEQQRLEKLKLEQQQQNVENEKKKKQIIEIPEEWSDDDQIIQVPKQQKKKKQKKQDFDDDEILEVLNSAKKTKVQSLEDLEEMEKKIIKKKKKREQIPQDEFVQKVPAQNQTFIHFNNKNNYLELAPCSNNPIQQRACSLCKSEVGYQLDDDNWYMIGLQHGYKKEFLLLIEIIKQSFGDSQLYLSFSSNGSACMTSDTLKQEIDVENVLQRFSGKSWRHFIKNELFALPQKLEGRSFQFKAIYQNKQFTFSDFEIKFTSNLIQELKSLSKQEVSIRTNTVEFIGEQLQCTLTGMMKQQNDELPFSLKFKNGETISELLIPNCQIPLHNEILYGVSDLYFGEYFKETLQQKLKGNFRGDFNATTSFENGKLKVRLAMKKFNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V2F5</accession>
    <name>A0A8S1V2F5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0540099</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8171075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V2F5"/>
    <dbReference type="OrthoDB" id="302114at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="150"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8171075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="347" mass="40720" checksum="7519A2AAB1C1C016" modified="2022-10-12" version="1">MNRQSNIKSELDQLTKHHTSVDKSKERNHTPKVKCCSNKYLLNSSTKQNLNRSYNSNQKVHSKLQSKATSDIKQKLLDEILRGSHSQFSQSYLTSIIQDKIVNKPQKYHTTDDTSSFTLPEKEFNIKTPFLQIDIFTAEECKKQQDSITITKFQELLEQLELKEVELKVNVFSNLKTLFLILAKQIQQKLASQMNEITKLQNFNTELQQQQKILQDRIQEQKQITIKTEETLNSLLFTLRYTNLNNPQIIEQHTDQVETQKHQSDNKNFEENISMMSEESAPFGKFYNYYIKKQQDPQNKRALGLTLNLNSLKNPQTAPIGYQEEFMANLNEFSESWRQQAIKEQRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V488</accession>
    <name>A0A8S1V488_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0560133</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000056">
        <property type="protein sequence ID" value="CAD8172180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V488"/>
    <dbReference type="OrthoDB" id="325404at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8172180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="206" mass="24118" checksum="088D3B267DB0AEBF" modified="2022-10-12" version="1">MYTALIKNGIYIPDDNSAAISSEYLSNVALGRCFKIQSQNLRQGQVLKKISKLRLCIELDGFCWKKQFNLGLSEDRLPDKKWLLNLLYSFNPENKNLTYYQDCDNVFGLTKDDLTQIIISQKSFEIVGYVFGSAKRYEIFQNDFRKTCFESNKINQEKNNENIIQEQIKGYEWFQKNKLIPRLINSRGLAYEKAFQVVKDEMNTQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V6Y4</accession>
    <name>A0A8S1V6Y4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0590077</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000059">
        <property type="protein sequence ID" value="CAD8173368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V6Y4"/>
    <dbReference type="OrthoDB" id="283467at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00882">
        <property type="entry name" value="Ras_like_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8173368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="187" mass="21559" checksum="195CB9ACB14D8CED" modified="2022-10-12" version="1">MSQVMLVLGPSRVGKSSLIDALTYGQFHREFYPQDIDFQVKLAKVAQHRVYEVQSIEWDGKPFGLVQFYSNVADLILIVVNICGCYDQMDQIHDIYQALPIANNKQQIVLIGNLNGQNKRNEKLCQSLSTFAKLNDLKLYELDSTNIMNVQMEFSFITQPYSLFDDIWNRNQNYVTQFVSQAVSLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V8Q0</accession>
    <name>A0A8S1V8Q0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0580170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000058">
        <property type="protein sequence ID" value="CAD8173011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V8Q0"/>
    <dbReference type="OrthoDB" id="295761at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="407"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8173011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="482" mass="56383" checksum="D92E52E520E19B66" modified="2022-10-12" version="1">MFLFLIYGIFGQVIVEINDNNAYFNVHEGVRSTILQLPHAWDGQLNGTGCELRQKSIQESISQYQIIEFSEDNMEELILVSQDDHRIAIISTNLRLVIIHKNNLKEKMSYDLSKYEINYIHQIILNDNILLLITDTKAFVVYLQPLSIIEFAEWIPRQERWLTNIYGDFIYTAVSLNGIDIYHLNNLTYYRTINHVDIGQNQLCVKDFTIFKTTLYILDCYMGLIIAKMNSYENVIDFKVRKIDVQEGGIAVDTKNGINIFVGYKWKLRYAIVEYVVDDTEWRIQNLIYTNKIKDVDVNNEYAIIQGNSRHMVAFNFGTSLSNDIIKYNLRDFELRNNELIGITATQLFSSQLQLIPLKIDCQIYQDNSEIYSLVYNVSYNGTTIERHHLNFQVNLVSTYLYQHQSYLVIILITLLFCIFTLIIIMIFKWYYSLRKEERKIAKSIKGYSLSIPTSGRTNTQQSSRPFSPSNQHLIINNIQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V9M8</accession>
    <name>A0A8S1V9M8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0590185</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000059">
        <property type="protein sequence ID" value="CAD8173557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V9M8"/>
    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052276">
        <property type="entry name" value="Diphthamide-biosynth_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44240">
        <property type="entry name" value="DNAJ DOMAIN (PROKARYOTIC HEAT SHOCK PROTEIN)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44240:SF10">
        <property type="entry name" value="J DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="390"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="178"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8173557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="445" mass="53487" checksum="CD18288403D346A4" modified="2022-10-12" version="1">MLIYKKATPQELQKYRDDQVKNWKKEKDLQRLSESQVNQMLDLFLLFASQRTFTIRTKDVEAQNTLLRVNEESTLFQNVLSKMILIDEDLNFINFLKLYLQSIGEAQQEEQLKQLFTTLTAKGTITLEDLKKLAQELDHYHLKEEDLNEIMKILYTDSEKLKIIMIKIIYRFSTQQINYYTILELNATCTQKQIKQNYLKLAKIYHPDIYKGNDTNRFKLIQEAYNTLKTPEKRQSYDQTIFKERAFANQERSESSESEETTQKNEYTAKQTKDGINFTFTKKEYKEATEEKTVEEEFRKFMQEPLKINPEEVKVYENVVARQMTKEHKAQDEFIIAKENKWKIFFFQAHQIGYAQQVKEYIKGINQQWEQKKNDTEEMRQLKRQMQKRIANTIFFIFIGLSLPFFYSALARRQEIDKDFKEDLDFLYKVADQMDYLEISTRFVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VB67</accession>
    <name>A0A8S1VB67_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0600050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000060">
        <property type="protein sequence ID" value="CAD8173775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VB67"/>
    <dbReference type="OrthoDB" id="297705at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8173775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="169" mass="20267" checksum="FE778183F53C8D62" modified="2022-10-12" version="1">MDNLEQNAITQLLNLEDKEIQNAWDINSPIQIHKSRSLVQLRQKKIHQPIEQRKRIDVNHECHFHKFPKIYQQEHSFKSLMEEYFKQLNQKQLNLKKDVQKSTRLRHSSYLIEEGANRKSKHSCHEILLQDNSSNKQIKLSDLCKDIMIYKPLKKSSYFPRLQPSLHLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VBE2</accession>
    <name>A0A8S1VBE2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0600086</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000060">
        <property type="protein sequence ID" value="CAD8173845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VBE2"/>
    <dbReference type="OrthoDB" id="10310511at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8173845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="67" mass="8245" checksum="F0C217FD0A36774D" modified="2022-10-12" version="1">MEELRKSIIKQEEEKMNNKLTLLEQSVGYPLNEYMNQEFINKIPEDSFLCYERRKTIKTRYQRTQII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VEH6</accession>
    <name>A0A8S1VEH6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0620246</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8175247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VEH6"/>
    <dbReference type="OrthoDB" id="287121at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04048">
        <property type="entry name" value="C2A_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000348">
        <property type="entry name" value="Copine family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045052">
        <property type="entry name" value="Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010734">
        <property type="entry name" value="Copine_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857:SF106">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857">
        <property type="entry name" value="COPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07002">
        <property type="entry name" value="Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="37"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="3">
        <location>
            <begin position="325"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8175247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="804" mass="92867" checksum="A7F4DD9D49DBA016" modified="2022-10-12" version="1">MGSGQSKTKKKAAQLKQPKIQNEELKRQPQYEDDVIDAANLAIAIQKEDLTTNIELFVHCEGLPKMDAFSLTDPVVVAYYEKDDKWVYIDRTEIIPESLNPKFVKTFIVNYIFERKQKLRFEIYDIDDFEAIDNLERQEFIGFVECEIRDIVCAPTQSISRTIINRKSNRKRNGLLHIRGAEYDMGGNTILFQIGCQQFTTKAEVLLRMSYFTDNKDWMPIHATEPIKYQKKQTVQFNDFYLPLSKFGNNEQAQIKLEIEEYNKTKGTTQLGECETTLQQLMENSGKTLRMTKNGLIVGQLKLIQVKQHSRYSFLNYIYSGAKIQVIVALDFTNSNQIINEEGGQAELSTSNDYISALKQILGILQYYNNENRYPVYGFGAKLPPYYNVVSHCFACTGNIFDPYVYGEVDEIINLYKEILQGVVLHGPTVFSQIISQAIEFAANEKVDQNNQKYYILLILTDGSINDMQSTIEQIYRAQEYPLSIIIVGIGKEDFNNMRILDGDDPQHKLRSKIHNDDIKRDLVQFVEFNSVRDNQDKLAKETLAEIPRQFLEYMEKNKIYPAKGKKNTKAATNDFIHSKIDEMKKLKKKEGVISGKIPQFLNQQKEEFIKQLVQLGYDRLAVTNVINNDGVPCKDVNLVIEILSQKQKQMQIQHPRDGHFLSKRSEMAQKLLANVSSSDSKITHRVFCIRQRNKIDELMQLKKQESKITATNFLFKLTEQSKPKLKPVVEQKRQIIESQNNYETTKIGQSKENLCLNCDQNKIELVFIPCGHACSCLQCYKNLQYCISCKQKVQMFAQIDYKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VHR9</accession>
    <name>A0A8S1VHR9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0670194</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000067">
        <property type="protein sequence ID" value="CAD8177408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VHR9"/>
    <dbReference type="OrthoDB" id="1700726at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF33">
        <property type="entry name" value="AMP-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="3">
        <location>
            <begin position="59"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8177408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="669" mass="75433" checksum="344A09F574402E07" modified="2022-10-12" version="1">MGCQVSNISPFMSYSTKVQEAKQGQSEIRRNVKYTNQLLTIPEQGVMNLQDLFLKSADKYAVKQCLGSFIGNDFSFMTYSQVQEEAIYLGSGIKTWGLANEVNEYMNYKMKLIGVFGKNRREWVILDIANILYGYTMVPFYDTLGPDSIPFILGQTNIETMFLSADATKSLLKCKEKYKLQNLVLFDQLTEELERELKNKGYKLFRYEDVVQNGKSQIQPLAQIYPETIYTICYTSGTTGNPKGALITHANFVSAVASTQVTDAQINENDVHLSYLPLPHVMERLIVISLLYVGAQIGFYRGDPNLLKEDILKLRPTIFVSVPRLYNKFYDGIKAKMAEVTCLKKAFLDQAIKSKLSSLRSQATYTNSIYDKVFEGVKQLFGGRCRLMVTGSAPIQQDVIDFLKIAVCCPILEGYGQTESSALSFSTARWDPISSHLGGPAANTEFKLVDVPEMNYTANDIVTIHKGEKEEKLAISRGEICLRGPGVFVGYYKDPQKTAEALDSEGWLHTGDIGMITEQGGVKIIDRKKNIFKLSQGEYIAPEKIESIYNRVQGVAESFVYGDSLQNQIVAIIVPQYDYIQKQANLMQIQGGFEELCQNQKIISLFKQNIEAFGKQNQLNSLEITKLIYLEPQPLQNLGCLTSTLKLQRHIAKQVFAKQIENLYKQQVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VKL2</accession>
    <name>A0A8S1VKL2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0680079</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000068">
        <property type="protein sequence ID" value="CAD8177780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VKL2"/>
    <dbReference type="OrthoDB" id="295194at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8177780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="471" mass="55716" checksum="5452C7AF38111B5D" modified="2022-10-12" version="1">MKQAILDFINSGILITKPIDAFYTEQFENFTNECYNRLIAFYMKQQSDEKLKSAYIALMKVVQNPKVENLNLLVFCGAYIAQKTYYYQQNLIQLNTKNIERIHLDCYHIITFTLNGVIVSDQYIETNLKKYFTDKYLDYTKKTAPPPQYVKPTKFFGRPIEYTKLESDEGTEFAQTLSGIFKPPIKHKKLPVVEEQTQYQVRQQFKSPPIRRMKIDINSISPSLATALHRTSLGFEKPKIINHPILKSQLGDMNFMQRLKDLEQDGEQKLETKIQRSHRQNIVEQHNLTYPPKEYFQKNVKVDALFKNFLELKYVLDQRKETMSSIKDINDYQPRMPYIPTYTPYLQFESPQPQKQQLIQKSSFEILPPSHELDKATINKFDIQAQTTKSTFNNQINSTFFFKTNNKPQSFSKHSTQNSFFQYMNKQKEYKQKFDRFLSNESSPDRNINQINSQLLKKQNFLKKSLQRLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VKT7</accession>
    <name>A0A8S1VKT7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0700011</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000070">
        <property type="protein sequence ID" value="CAD8178598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VKT7"/>
    <dbReference type="OrthoDB" id="286708at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF271">
        <property type="entry name" value="MYB DNA BINDING PROTEIN_ TRANSCRIPTION FACTOR-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="83"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="147"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="2">
        <location>
            <begin position="154"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="210"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8178598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="516" mass="61527" checksum="69931673BB0983EF" modified="2022-10-12" version="1">MNTQKNTKNKNDHDVPSNFEMEDLKVVSQDIVIKEFYHFIEKTSFIQENFEMLSKVGDNLRLDFKDWPTAEQAIVLFKLNLQNGRKKSIKWSEIEKKLFYWIVIRYCLYKGLKDNRLLKYEEWREISKIILGRNAHQCRLKWEQRYRVPLSQIPWTEQEDQLLFSVHQEFQKSGRENKWSQIAKEIFKQSSNKVFRQPKQCRERWINRLDPNISNVPWDKQQEIDLLKMILIRGKKWSELSILYGRARTENTLKNKYNSLIKREIQKQEINSINPILFEKVQILRQNYSKKYGISTPIEEIDNFECQFILLAIKNLYVELYLQEGKIKEAQKMNKDDFFDYLPDDSLQINTNERFLYKKKLNIQKVDSNFYVNNDHSSLVIFNKRNNRIYLTPYNVLDFQNIILNTIIKKVNIDINTINNTKEQATLSNQSQFILANQQNCLIPCQQMLQNYLFVPNCFGQSLSSQTSNSFSFCTLQQSQQYDQLKSIDLSNQKYQKDMINSIGGDIKTVDSCDEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VS06</accession>
    <name>A0A8S1VS06_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0710053</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000071">
        <property type="protein sequence ID" value="CAD8179053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VS06"/>
    <dbReference type="OrthoDB" id="10258955at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8179053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="77" mass="8452" checksum="2D4A978C04A2A7C8" modified="2022-10-12" version="1">MNNIQGYGLDKPSVRLHQAPGGNSSISFGDYTPQQQVPQPLGRKQVNQPPIQQQQQDVKTSVKVRNPPGGRSQIQFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VST4</accession>
    <name>A0A8S1VST4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0700130</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000070">
        <property type="protein sequence ID" value="CAD8178832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VST4"/>
    <dbReference type="OrthoDB" id="320819at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14014">
        <property type="entry name" value="STKc_PknB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="14"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="415"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="569"/>
            <end position="651"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8178832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="786" mass="94227" checksum="7DF4647486BC1B0B" modified="2022-10-12" version="1">MIEAPNDKFPQRRFVIQTPLGEGSQGKVYLVKAINWGINDQKQYAIKQQININENEQATINNIINYQKEQSNPPSQIIKTYEIFIQNNKVFIVMETGEMDLYKYLSSKQQINLEEKIRIMKQLSLSIQFFHENLKLVHRDIKPENFIKVGNEFKLIDFGLAKKPQDRFMTQNVGTPFFQAPEQIQGQNNYTFSVDIWSLGCVFFEILKGQTLFNANNIQQVQNLVLNYHQKYLEVQLNQNDIPQNLKSLLIQMIQHNPKDRLTIQEVVGKLNQMQTEYFNNVINNQNQNKNLIFYNNQQQKMQVNQQFQQQINFAQINPINQQMQFNPQIQLQIQQQLKDQEQKQLEQNNLLQQMLSILEKQNFQEQLKYLQDQQDKYQQNISEQIQLFQIQIENTLNNQKNENNQQIQIQNNKNENIFNIIQQQKQNISDLEIKKLEQDNLISKQQQIINDFKIKIEVQNTQINKFKQNEIQQQQQSQQLQNDIQIKQQLIEQQEKNYKKLQEELIQLNQYIILYKSQENVLQMTIQQNEKRIQEQQDFIQNQKIQQQQFNQNILDKLQQIQIDSKEINKLDIQINQIYDQLQKQQNQLENQIKNIEQFKIQIQMQKETEKQVFEKVQQVKDKDKYEQSISQYKKQIDELNQVIQQQQEIMLKYEYYYVPHTNSNNKILENIKNDILFLIEKVKNKQFIAHSYLDSTISALPQQIKKTVNGLIDLFINDINNMINFGYEVRNQIKNLIDQSKLYNVKYDTIFQVELDNLKNFYSILENTQNQFIQSRKSDIRRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VT50</accession>
    <name>A0A8S1VT50_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0740144</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000074">
        <property type="protein sequence ID" value="CAD8180480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VT50"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:001086">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF206">
        <property type="entry name" value="HSF-TYPE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="3">
        <location>
            <begin position="9"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8180480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="264" mass="31714" checksum="2FFD3A73EF173772" modified="2022-10-12" version="1">MNQKNKQYKTIKFIEVTYEMLNQKENSDIIRWDEDGMRIQILDRELLQEQVLPKYFRHANYSSFLRQLNMYGFKSSKDQYGSITYYHPSFAKQKVLMRNIFKKKHQKEDKNDTNSFFIDQSELHNQIKALQCEQVKIQQKLLSSIEYQIKIKHSIKLFLQTQLSLAQEGEKNCRLFIESIRYLVKGMNCESQNTFELLLKQLFPQFVQEHPQQIQVINEMEEQSIQYLFSPTPFGRIDFDQEYISFTQTSLELEREFSGFGFEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W2Z4</accession>
    <name>A0A8S1W2Z4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0830030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000083">
        <property type="protein sequence ID" value="CAD8184374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W2Z4"/>
    <dbReference type="OrthoDB" id="308431at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="15"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="176"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="296"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="472"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="594"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="678"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="792"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="961"/>
            <end position="1019"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8184374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1120" mass="133513" checksum="DF24D89FBE49942A" modified="2022-10-12" version="1">MQQIEVICFGLAVEVERLQSLNKQLNQDIDQANLQNKNSQTLIDQQTIEIQLLQTKLDDINTDQIKLLNQYKSLQQQHLHITDLINQITAGQITLEQLKELIDELKNRSILYDEIQELLQGQDLQDVISKAEQFQLQCNINQELRDQLEQYPEIVIKLNQDLDKHKQDLFNNKQCITNMEQTILNLNQEIHILKQLIEQEQYKNSSNQSQMSLSLETQLNELRLKLSQNEQKTEELEDEVEQVNFFKAQMESQLQEILKENNKILIDKEKLTSDNLSLNTLNENLQYQLNIQISTNQDLNTQIIRLEDQVRLFKNQLAEENDLKIQIIRLDAQIQSQYERIQMQDNEIQDLNLKNQEYKQNNQQSLNKYQDLFNQKDNEIYNLKQIHIELQDQISELQNQIKNLKLQLQVDADEINQLRILHSQFQGQNDSSNSIIINFQTQIQQFNQEIQNHILKISQQETIILNQQQQFINEKLELQKHYQQIIEKLQDENKSLLDQNDQQFQSLEDQGNQILSLSSENQDLQEIIDQKEQEIDKLNLYVKKQQEIHEGNIKKNEAFEEQNRELLFKQSSFQKTIKDLQTQLEGFKLEIIEKDQLKQKKLQLEQDINKLNQLNAELNQQISTMKVEINNYQIQINNLNLQINNNEIELRSSFQENNSSENQIKELFIKIQQQEIIINQQDSQIKEDEFRIQQLMDELQKLNQKSKDYYKKFEEQEIFIKQLQVELNELSNDIKKLENELDLKEQEYNELDDEYNQIWNQLNAFKNDQSKQYSDTQIQELKQQLTNSKFEQVKLLEDLEKLKQQLVDKDSQLNQKAMVIDKFNVKSMNQINEIRLLKQEIENNQIVIENYIQKISKQEQYIQDLLKSLESEKQCQKSQVKDNTDNQRVFELQQLNMNLSKVIDEKTQKIQQQEQEIIILNEKIRETQIYIRSMEIQFNELKLKYEQQIQLQTTYQKSSFIQNSEDNFDELQQKYQQLQQKYVQLQRDYQSLLNNQTKLEEYENKIALLSLEISRYRNSSKKSTPLINREGSSSVKTSKFNLGELQSPIHEELKIEQSNSSQILIQPFNNPKIEEQYGLMVLLFAEIEALRMQDSNRQTIQDDKSKVSCLMDYYRTKKPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W333</accession>
    <name>A0A8S1W333_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0740172</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000074">
        <property type="protein sequence ID" value="CAD8180536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W333"/>
    <dbReference type="OrthoDB" id="302433at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="82"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="129"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8180536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="212" mass="24901" checksum="03034A275327DC1F" modified="2022-10-12" version="1">MDLQHSKSYAALSYQNSQLFKPKPLSFRQQMVQLSTETPRLQIQALTTPTIMEGDSNPYVNQQPYSFKSYRISTEIINGSPIDQIQLILRENENLKRSLNQKQQIIESLSRTRKIKPRLDFSDLKKNSRSLVQPQSQQESMKPKNIETKQSISQNYNKRIKLPKIEDSVQIKEDECNFTFANNFFNNNTCKNQKINFKQVFTQSHLKKKFFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W4T3</accession>
    <name>A0A8S1W4T3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ras-related protein Rab-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Small GTP-binding protein rab1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0810156</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Protein transport. Probably involved in vesicular traffic from ER to Golgi.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000081">
        <property type="protein sequence ID" value="CAD8183747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W4T3"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001018">
        <property type="entry name" value="Rab family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="197"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8183747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="208" mass="23529" checksum="D2CA478A664F150D" modified="2022-10-12" version="1">MSRQANSQDYDWLVKVIVIGDSGVGKTNILSQFCDAKFSITHMATLGVDFKIKTIDVDGKKLKLQIWDTAGQERFRNITKTYYKGAQGVILSYSVIDRQSFQNVDGWLRSIQENTNSSDVQLVLVGNKADMSAERQVTLEEGKKLSQQFNIPFFETSAKSNLNINETFHNLAQRIIQTLSKMQANDDKQNLNLKTPQPKDKPKNESCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W798</accession>
    <name>A0A8S1W798_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0830074</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000083">
        <property type="protein sequence ID" value="CAD8184462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W798"/>
    <dbReference type="OrthoDB" id="285371at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8184462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="379" mass="44423" checksum="0BA24B49E273B828" modified="2022-10-12" version="1">MFFVHETPEIIDNVQFINLKNKVGLAQEDQQFLQNLEKSRSEVKYILSHSNIKTLVENRVSLLTKKDSLDNYLKTNVDMMRLYLIKIKEHPKFSYKLTINKKQQQYKTEQINTAEEACGCEIVMVRYMYLLGFNNSATPYVLRLRYSMLSTPNNIDLNDIQEALKIISAGWMPFQRLCNVMDDLLHFLVTKQQQLIPELKMTTIFSFKLLIECNMNFFYLETFRPQKQYDNMIKVCKSSYTKLIDAYNRLSQIKDEIHPEILEYCLSGQKYFKVYGIWSILLKNESDILFDRTKNKSYTILTAECHKIANQIVQTAQELSSIKNINEYDKFTLGFIQNVVNPTLQKSLSVYSQSAKQPCQLPDQLLVLAESLDYTDQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W9H3</accession>
    <name>A0A8S1W9H3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0850175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W9H3"/>
    <dbReference type="OrthoDB" id="298920at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8185597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="393" mass="46065" checksum="18B6C3AEA255FD9C" modified="2022-10-12" version="1">MELLPINVIAQILKFLNISEIYSKLTLSKHLIQLLQNKQIQRLILSIYYPQQLMIKVQQDQYMSLIKSLSNITLQEIPFWGTETNGGLTDNSLRFWIGKSFMNTDSQQVSILRLENSFISGTLAFDSINLKQALVQYTKRFIEILGMELSQTFYTSNSVNISANKIKIYDLFLSFQYLEDFEDQTIFDGLEYLYLYQYCKNLQIVLMTSQDNYNLKDDDHQILPLAISLVQGVQICRKTFSDKIIKTILLITSEDQYIKENKKYNLKTVDELYKLINEDPNQYSPAQTNISLLQNFSDQTQFDNSFQNEYIYCTFEPKGQLQPLIWLQFVNPNTYEITIKFNQFTQRLAKSIQLKFLEIDQQEQFEYTGISFGYISIQGYVLDSNDLNILKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WAB1</accession>
    <name>A0A8S1WAB1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0840026</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8184829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WAB1"/>
    <dbReference type="OrthoDB" id="293068at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="100"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8184829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="458" mass="53802" checksum="617CEBD1E38E4C62" modified="2022-10-12" version="1">MIFDCQVIRKHLLIDKYYQLQFYEDRILIGQENRHPTKYEIALNLTNRISWKLNEKQQIIEFGIKYNNNIKWFTGKNENLQRIKQLLTNKIFNHSISTFYQSSELIGQGASSKVITILDQSKFKYAAKCISKDYITKKKTPDRLNRLISEITILRSLLNHQNVIQLLDIFEGESTYYLIFEYLQGDTLHKFLKQQSKPLSEEMIRIILAQLLQGIHYIHSNNYIHRDIKLENIVLSTKQCIQNLKIIDFGLAIPSTVFTPFAICGTPGYIAPEILQYKESSQGQFRFTSKVDMFGIGVMLYRMMTRKSLFDSENTKELLSLNRKCQLPKLSISGYSNELNNLLFGLLESNPIKRLSSEESLIIMNITQMTQELQLSQFTTFQYDQDDFEVEHSIDYANAPYQFPNKFMKYELENSFHYNEDHKHSSLHKSFKLSFSLRISQEIPKQDFVIENFQNIPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WAJ5</accession>
    <name>A0A8S1WAJ5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0880221</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8187248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WAJ5"/>
    <dbReference type="OrthoDB" id="313274at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0085020">
        <property type="term" value="P:protein K6-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF8">
        <property type="entry name" value="BRCA1-ASSOCIATED RING DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="256"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8187248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="370" mass="43255" checksum="8171CEAE8895711A" modified="2022-10-12" version="1">MKFHLISPTTTSFTPIWTSQQQKRPQIKLSELAKFTQLQLPKQEDSQKAIEYKQRQQQLLRQSKNKPLLKVLTKSQLLQGYLSMRPQQQQKKQIKSRSSSLITKLNSSSPVVSINESPKIVKGECQIMNQPMQQIQLIEEQQLSPKVLKPLKQETFAKALDLIRPETPLNFRCLVQDISYKKHKINIKAIIRDIINKLSILRKLDIKYIDRRIWSNQPFQKRNQYSFIKACKAGDLNQVKELLEQDRFLNFGFDYIHQTGLHWAVKRSHLQLVKYLLKIGCDVDAQDLIGRPALYFAIEQKDANIVKLLLENNAIPWSNQKITYSKLCNDDLQIMLLLNKARKVHVIYRMVDAKKRIQIKQRALTGLFDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WAK0</accession>
    <name>A0A8S1WAK0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0790168</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000079">
        <property type="protein sequence ID" value="CAD8182846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WAK0"/>
    <dbReference type="OrthoDB" id="288291at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398:SF0">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="336"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="636"/>
            <end position="663"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8182846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="663" mass="78491" checksum="B887E0F37EA1B34B" modified="2022-10-12" version="1">MIQKCKQGLRQIDIFGQTINLSFRQEEQYKTSIGGFLSICMIATIISFFYSNIINFFAKLNVTSTSEFTFAKDPDKLELDRNHFMFAVQIEQDNFTTNPYFNITVEQRHYHRYPNGTHYRYPNIYIDLVPCTIDHFSHLFELYNVNFTDQFEEQNLKNFLCPNLNFIHSQNMTVGGVWASTDYYFLKFSVTKCINSSNNNFTWKPTCKTQDEIESTLNYQGSFRFQVFTTNFLINPNRPKDFVQPFLATDQFYTFVPDKMFVQSDIFFRTKKVTTDQGILMYPDKQNETFAFRDQGDQREQFEISRITPNYYGAFYFQRSPYSYQINRKFLRLDELLSYLGGFTQFMIVVVGILVRFYNRQHLIVSIANDLYEFDMSLNRANTQMNFNTLLEKKERREIKKQKTKNNHQGLPTIQVMSPFSHKPDMLQNTTPAKDDSQKFENKKSIEYVQPTIQRVQTRLSILKVKAFEFFDDFKEFIKKKYIIGLGFRVILSQIVPLESIKNNDCVVLQRAIDQVNKELDIEYIIKQLHELAKLKKVLFTEEQITLFNFSRKPKIALVQEGKKRRSTKYIVNDSVTTEEQGLLRQFNDLVTSYFKIMGQDSENQTEEQIRFNQRLIILLGSELMNVLEKEISQQQHLDSENNECKEEENLISDKEIQIEKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WBW1</accession>
    <name>A0A8S1WBW1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0850095</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WBW1"/>
    <dbReference type="OrthoDB" id="298278at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8185459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="232" mass="27968" checksum="38144F1FCF600AD5" modified="2022-10-12" version="1">MAIYFPEESDRINKKAEQTKQKILEMRKIAQEQFGIVFPDSNPFQIKTFEQRDSIKELDENIRMYKKIQKNNDRVFIDFEKKNQYMFDQNSFKTLQFIKSQKQVSEEKIETSCQSYQSLVNRTLKSVLSQRFDKATNVSKKSSPRTIFQKDRSCTVRTRTITLTKRKTMNPQKVEFEGLINEQRRLLQTSHLKRYHKDKLQQDFQNLLAELNNIKIHKEETIIEKYKKKYLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WEM0</accession>
    <name>A0A8S1WEM0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0870161</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000087">
        <property type="protein sequence ID" value="CAD8186609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WEM0"/>
    <dbReference type="OrthoDB" id="282579at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8186609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="107" mass="12545" checksum="89BAA75F36C8F6D5" modified="2022-10-12" version="1">MDSEQHQYIKLKFVILEKDWPYPNFEIVVAYNDPLMFINKELQDRHGGITDIRLYLDAEKQNQLQYIFNKKIGDALSIKGSNSQDTAPIVTIYYDFKNAIKSPVQLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WF07</accession>
    <name>A0A8S1WF07_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0880218</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8187242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WF07"/>
    <dbReference type="OrthoDB" id="311870at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45153">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45153:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="273"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="380"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8187242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="428" mass="49757" checksum="A0AA610C82B2B8CB" modified="2022-10-12" version="1">MKKILQEKIDKHLQNAKQLVKTDVHEAIKEYYKILFLNKNHLQSYQDLAQLYVKIGDYSPAIACLQYSYSVKQDILVEDELQALILLKGQSLLEQGDVTNFLEGTEFEQFGYLIDFTVPSLQKAYAYLLTNYLNYTLQEVQKVIANDPLNINALLLRGKLYWAQGKIAQGNSDYWKVYSMNPELQEIKEFILYITPKIQKLTNDAKLYLVQRDNERCKLNVDKGLELDPNNVDLLLLNAYLCRINIKYDVSLNLLKKAYDQQPTNEDIKKQLAITYNEMAQVMFEKGNYEDALTLLNEALTFKPLDWGILVNRGDAFKYLGKLKESICDYNKAYEIEKNDTVAQRLAIIHHSFGVEFFNKKQYQLAFESFDKAIKLANSVDILFKRGKCLLYLQNPQMALKDFEKVIQMDPNHYEASTLVRQMTSSTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WPK5</accession>
    <name>A0A8S1WPK5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0970063</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000097">
        <property type="protein sequence ID" value="CAD8190680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WPK5"/>
    <dbReference type="OrthoDB" id="294775at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8190680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="236" mass="27625" checksum="868B57647B7CC7D0" modified="2022-10-12" version="1">MLIIINKRNQNRKTTQKLKYLDSVQEQPRRASCCCDNCGKLTHFQQIYQNSPLVVQQHYVQKQQQSLLIECKPLKKMISTSFHLSNKNTTPIKKRRHSCKCQQCGQETLFQKNTCYKIPIRQPIKSPHFKTFIIKKSLGKVFQEFSDVKKTYPLTSRSHKDVLIKSPILVNRLKTGSCLLPLQNSQSFKYQVKTNKQLVELNRFKNGNSSEKCKYLVNKHEHIIPTTTIRLKTIFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WQA1</accession>
    <name>A0A8S1WQA1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0980010</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000098">
        <property type="protein sequence ID" value="CAD8190977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WQA1"/>
    <dbReference type="OrthoDB" id="309679at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004520">
        <property type="term" value="F:DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18010">
        <property type="entry name" value="DEXHc_HARP_SMARCAL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000073">
        <property type="entry name" value="DNA helicase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010003">
        <property type="entry name" value="HARP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45766:SF3">
        <property type="entry name" value="DNA ANNEALING HELICASE AND ENDONUCLEASE ZRANB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45766">
        <property type="entry name" value="DNA ANNEALING HELICASE AND ENDONUCLEASE ZRANB3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51467">
        <property type="entry name" value="HARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HARP" evidence="7">
        <location>
            <begin position="10"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="142"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="427"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8190977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="763" mass="88126" checksum="90FCFD8C08A3A842" modified="2022-10-12" version="1">MQFLNPKKQKFQQIQKQEFNFQLLDENRVFMTGTFTAFTDNVKQYIKQNDGSFQNLDGEKGWIIPIGNYQNLITNLKSEQSEVVIKQLPQLLQKLQNLNMKEIKYFENGQLVTLKYDNQIRYEQLDKTLDETLYQYQRDCVKQGLKFNGRILIADDMGVGKTVQSLALASMYKQNWPLLIMCPSPLRLNWQDEIIHWLKLHKTDIQLINCGREGIRMNAKIVVVSYDICAKIKDNLMSRKFQICIADECHYLKSPSAIRSQVCVPILRQCMQTILLTGTPALSKPKDLFNLLNIIRPDIFGNFKEFGYRYCDPKLSRFTKGIDFDGASNLKELHFLLRNYIMIRRLKKDVLSQLPEKRRVKVRIPGETSQVKQIGALLNQLGNIDIQQLINKDAIFQESKDQSEQLLTINSILQKCYMLTGQAKIKAIKDYISTLFENEIKFLFFAHHQDVLDAVQEYCIQNEIQYMRIDGNVGVEQRHLNVQMFQNNDEIRIAILSVTSANYGITLTAASTIVFGEMHWTPAIMMQAEDRAHRIGQVQCVDCHYLIGDGTLDDHIFNKIENKMNTVSNFIDGQNQNLGALEFSADEVLVKGTSKPTLISDKGQSVLQEGYVQPSIQSLTEDDIEEIYQLLESRSKEKQEQQEAESQLKLNQKKKQVELSSQVKNQCSTLKQQLLDKFLTSNKIKKEVIEDNKEFNKIQKVIEENKQLTYGKLPQKLSYEYLQSRLNGDSKSTESGQKKLLLKLTKQQPSLNSQEDVIDQQVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WR38</accession>
    <name>A0A8S1WR38_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphatidate phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1010023</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lipin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.4" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDO010000101">
        <property type="protein sequence ID" value="CAD8192203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WR38"/>
    <dbReference type="OrthoDB" id="4567at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000515">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026058">
        <property type="entry name" value="LIPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031703">
        <property type="entry name" value="Lipin_mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007651">
        <property type="entry name" value="Lipin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013209">
        <property type="entry name" value="LNS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031315">
        <property type="entry name" value="LNS2/PITP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12181">
        <property type="entry name" value="LIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12181:SF12">
        <property type="entry name" value="PHOSPHATIDATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16876">
        <property type="entry name" value="Lipin_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04571">
        <property type="entry name" value="Lipin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08235">
        <property type="entry name" value="LNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00775">
        <property type="entry name" value="LNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LNS2/PITP" evidence="6">
        <location>
            <begin position="420"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="318"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="318"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="332"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="342"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8192203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="621" mass="71899" checksum="4D51EA53F3608855" modified="2022-10-12" version="1">MTSITQKIISKFDPRQYYLSGCIDIIVVQQHNGSLKSNPFHVRFGKYDGQDYYVDIIVNGNLTNVKMRLGKEGSAYFEKSSFSSGYQSDDTFSEKNSLFDEASNFRLSHNYKTPDDLFNSDKKNSNWLQTLKFWGKGSNQKKDDGVKIETTNNLKEFQQVDTSSLGLSQIQLQKQFQQQQSEKHIQTNDFEKDNITYSDKETLSPRQSVMELSLCGQYQIQQQNLTQTQKLQLFEQHKVSFSIFEKDSIKIINHKDLVFKIGDKFFSREAGIIQLLAKQVYNQEMIIDSLDQQKKQNTQQQQQWYSVLFGKKKGSDQIEQNTNSQRLNNNEQSQKQHQQFKSQDRLRKMSEDSVDTFSTTSIQKRRKSQRPILKPHSSILKQLGLQKGANKITYRLCIPKKNDIVELHGTIYLYDQKTKLVISDIDGTITKSDILGQLMPKLGTDWNHDGVANLYQNIQSMGYNIIYLTARAIGQADQTKDFIQNLQQQNTKLPKGPVILSPDSLFPAFKREVIDRTPELFKITALKEIRNLFIGESPFYCGFGNRITDSTAYQAVNVDISRIFIIDPESNIHKYNTDEITTYVEMNKNIHLYFPPVDEGEYQSQNFWKIPISDNFDINYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WSE5</accession>
    <name>A0A8S1WSE5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0990200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000099">
        <property type="protein sequence ID" value="CAD8191750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WSE5"/>
    <dbReference type="OrthoDB" id="77931at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8191750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="144" mass="16590" checksum="E42CFDA514886C40" modified="2022-10-12" version="1">MYKLESFWSLNSNNKALTPILIKVFTNYLQIISTISTFQLQVPAGLASVVGNPIELLAYSLDCFLFNIKDILIIYFLLNWNLIMTSSYIIVIFTIVAIEIITKQTQFNFSFIFTSSIYLFIFLQGNLIGRIISLLSYRKISDEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WTQ6</accession>
    <name>A0A8S1WTQ6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0930046</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000093">
        <property type="protein sequence ID" value="CAD8189126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WTQ6"/>
    <dbReference type="OrthoDB" id="303668at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="72"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8189126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="204" mass="24234" checksum="DD99B2525A86EF7A" modified="2022-10-12" version="1">MNQLVSIDSTLQANLERLSYLMAQKKEKKPDFRDQFQTNTNTKKIFVNLRAFVKQPQPPSPQSQNQKKEYKIPSQTITTQTQTPKTPTYRQQFIRPQSLAQLEPCIKRQEISVSNIMSERTDGKACYKNWVGLSVIERNDLFLKKKQQKLKKMRDDKDQQELSKCTFTPHFYNQIHIERPSSCFQNKSYQDIHKQRKNQNEKLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WUQ7</accession>
    <name>A0A8S1WUQ7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1050071</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000105">
        <property type="protein sequence ID" value="CAD8193844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WUQ7"/>
    <dbReference type="OrthoDB" id="294385at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8193844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="166" mass="19835" checksum="0CFB82D935E2854A" modified="2022-10-12" version="1">MQYFQILKPEIGISITRGILKGVVLIIIGKTILSYHPFDFQSDYPEYFARIPYKHPINPPIYAPHKMEEPEDREKYEKLFDNIIQISNNNREYYMFKHLYHPSLEDGFIIKEALWESDGERVLDEIRGEVRRQLRQAQNLELQNQFHITQRKVLFSKLNGQIRPSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WV63</accession>
    <name>A0A8S1WV63_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tlde1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1030176</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000103">
        <property type="protein sequence ID" value="CAD8193212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WV63"/>
    <dbReference type="OrthoDB" id="10039175at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021225">
        <property type="entry name" value="Tlde1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10908">
        <property type="entry name" value="Tlde1_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035890805" description="Tlde1 domain-containing protein" evidence="1">
        <location>
            <begin position="16"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Tlde1" evidence="2">
        <location>
            <begin position="56"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8193212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="187" mass="20747" checksum="E520A43E36228A78" modified="2022-10-12" version="1">MKYILALLFVAITYAGNCGQTCCQDLPKRSPMTFYYFINTGRFFGGSGDSSISTFGYSGAGKYRNDPTAQCLKNQGPAPSNTYTIKTCQNTMHSPPVDRPCSFWIDGNDQDKMCGRTEIMIHGCACCTPSDWSVPPIDGCSAGCIIINEENRKKLRVGDTLIVQDYDPLSENIQLDDIIIDKEIMYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X3B7</accession>
    <name>A0A8S1X3B7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TRUD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1080104</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000108">
        <property type="protein sequence ID" value="CAD8195059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X3B7"/>
    <dbReference type="OrthoDB" id="447290at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02576">
        <property type="entry name" value="PseudoU_synth_ScPUS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001656">
        <property type="entry name" value="PsdUridine_synth_TruD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011760">
        <property type="entry name" value="PsdUridine_synth_TruD_insert"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00094">
        <property type="entry name" value="tRNA_TruD_broad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326:SF21">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE PUS7L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01142">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037016">
        <property type="entry name" value="Pseudouridin_synth_euk_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50984">
        <property type="entry name" value="TRUD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRUD" evidence="1">
        <location>
            <begin position="326"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8195059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="635" mass="73975" checksum="EC510BBFAFF7C913" modified="2022-10-12" version="1">MESAVGIESFLQEGQPFKCIFKHRFTDFIVQEIDLKNQLAIISDKTKWLNFDQKQDHPQQQKNKTLPFANLSLSEEFLQQSTQLFGQEVTEQFKQLVEKVKTTGKCVQFETKVSLPESKEDRIKVHQLIRQNIPFLESFTKNVHNQKPKVEKKEDKANEKLQEQTLQKVQEQTLQDHILIKTSNINFKKYVASQTTSKAYGESKKFVKFLLMKRNIDSINAGMFISKQLGLSNKHFAIAGTKDKRGVTTQWATLQGMEIAQLIEIQERKFWNQNILMSDYSYQDQGIRLGELQGNKFIITLRFLNELTQSQQWELAKNIENLKLHGFVNYFGLQRFGSKNKIKTHQIGKEIIRKNWKECIRLIILSSQQEDEERVNQLFELDDFVTLSSIINPRLRIEKQLIDSLLQNGVHNYFTALQSLPRNARELYLHAYQSYIWNKMASMRLNQYGNKIVVGDIVSANVMDEGVADEGEEIIQQIAEDEEQQKKQTISACVLVTEQNISQFTFDQIVLPIYGHKITIPEESIVSTLLQQIFQMEQITKQDFEDASNKFFIDGNFRYFVQIPSEVEHQLFKYQNKDQDVVDSFGELLIDQGDYQGLLIKFKLKKSSYATMLIRELTKCPSTLEFQQEISGEFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X3I4</accession>
    <name>A0A8S1X3I4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1090144</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000109">
        <property type="protein sequence ID" value="CAD8195487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X3I4"/>
    <dbReference type="OrthoDB" id="306983at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000039">
        <property type="entry name" value="Splicing factor 3B subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000541">
        <property type="entry name" value="Splicing factor 3B subunit 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001513">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054573">
        <property type="entry name" value="PP2A/SF3B1-like_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015016">
        <property type="entry name" value="SF3b_su1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038737">
        <property type="entry name" value="SF3b_su1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12097">
        <property type="entry name" value="SPLICING FACTOR 3B, SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22646">
        <property type="entry name" value="PPP2R1A-like_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08920">
        <property type="entry name" value="SF3b1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TOG" evidence="6">
        <location>
            <begin position="748"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="44"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="95"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="106"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="120"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="147"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8195487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1176" mass="133157" checksum="558E2AE08180AE30" modified="2022-10-12" version="1">MTEEQGKQRFAYTAPKQILEEAAQIGEDNQTRKQNLDKVEEKFKYKQKNRQLSPERKDVFNDQGGENGRTYAEIMIQQDLENSRSEIENKIKKTETLNKEEKKLVKQQVKVEQNVSQQIKQERSEWEQESKDVSKKPQKWETPSRDGPQSSARASRWDNTNKLQATPGRAGTVFGETPTPGHMEIGDTPYKYGETPTPNRSYIKARWGERTPLAGQSGFGGGMTPHTPGTVMRTPMTPGQLGNMTPDRVYQFRLEKEMEERNKYMTDEELNSILPGPKDGYEILKAPENYKPLKSSLKKLLNAKNSIESPVQYQIPESIRIEVSATPSHPTIGQLPAIKPEEYNLFSALLQPINEDELTPEQAKERKIMALLLKIKNGTPQMRKSALRQITQSAREFGPAPLFNQILPLLMSPTLEDQERHLLVKVIDRVLFKLDDLVRPYVHKILVVIQPLLIHEDYYARVEAREIISNLAKAAGLATMITTMRPDIDHNDDYVRNTTARAFAIVASALGIPALLPFLKAVCQSRKSWQARHTGIKIVQQISIFMGCAILPHLKSLVEIIQHGLKDEQQKVKSITALALAALAEASFPYGIEAFDNVLIPLWEGIKTHKGKGLAAFLKAIGFIIPLMDVEHATEYVKAIVPILKNQFEIQEEEMKKIVLMVIKQCIQCAGIEAVYVRDQIMPEFFKYYWSKRTATDRRNYRQMVETTCEIAAKVGAAEILERIVGDLKDENESFRKMVVETIEKFINQLGVSDIDQKLENRLMDGVLWAFNEQQSEDTQTMLSGFGSIINAFGSRSKPYFSQLGGVLQWRLSNKSPRVRQQASDLIGKIAVCMKNCQEEARLGRLGQLLFECLGEEYPEVLGSILGGLKAIVNVIGMNKMSPPIKDLLPRLTPILKNRHEKVQENCIDLVGRIADRGPEHVSPKEWIRICFDLLDLLKAHKKGIRRATINTFGYIAKAIGPQDVLSTLLNNLKVQERQLRVCTTVAIAIVAETCGPFTVLPALMNEYRVRELNVQNGVLKSLSFMFEYIGPTAYSYINSVIPLLIDALTDRDLVHRQTASSAVKHLALGVQCLGCEEQLMHLLNHVWPNIFETSPHVINAVMEAIEGMRVSLGPGNILLYALQGLYHPARRVRLIYWRIYNMIYVGSSDACVAFYPTFPNDQYNSYDKYELNLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X879</accession>
    <name>A0A8S1X879_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1110137</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000111">
        <property type="protein sequence ID" value="CAD8196116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X879"/>
    <dbReference type="OrthoDB" id="291260at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001033">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="18"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="324"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="348"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="359"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8196116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="422" mass="49250" checksum="6CAF4EED02F656E2" modified="2022-10-12" version="1">MNTPIGKDLTNQVIGKVYKLIKRIGSGAFGEIYLVAKGKEEYAMKLERSDTKHPQIFFETKLYNYLQGSDPSDKGIPRIYAQGQDGDYNYIVMDLLGQSLEELFSKNNKRLSLKTVLMLGDQMIQRIEYIHTKQFLHRDIKPDNFLIGLGKKATRVYILDFGLAKRYLTKEGHIPYREGKSLTGTARYASVNTHLGLEQSRRDDLESLGYVLMYLLRGQLPWQNMKANNQKDKYQRIMEKKLETSPDQLCKGFPIEMSQYLNYCKNLKFEDKPDYNYLRGLFKDAFKKIGFEWDYKFEWIKDEQLVKTQQDIQSENKLMIMTQPLQQQQQQQQQGIRSNGIESDKKIGNTNSHHFNSNTQQLQSGQRRPQQQTSKVSQQLSVEKKRTTSQNKQFIQSKELVKPVTQVLAPKIVTTKEPHRKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XAR3</accession>
    <name>A0A8S1XAR3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1180062</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000118">
        <property type="protein sequence ID" value="CAD8198306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XAR3"/>
    <dbReference type="OrthoDB" id="304489at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8198306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="167" mass="20061" checksum="1557EC491CEFC1C3" modified="2022-10-12" version="1">MGKKKPDSFLSQLSKLFNGEKIIKLTIKRNLKEQYPQLRKRLRRKQKQQLLSEIKQVNDINEIIIRSQTQKLTISTSSNKQQINKFADSLSNIYRLYCHDQQKQIKKKPVLLPTQPNAKSKTQQRLKAKKLRNKERRQKRHELQKLKKKDLQRQKTFASKKREDQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XFV7</accession>
    <name>A0A8S1XFV7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1220125</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000122">
        <property type="protein sequence ID" value="CAD8199741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XFV7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8199741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="116" mass="13766" checksum="C9526E2528820E27" modified="2022-10-12" version="1">MLYSNYTLKQIKINFFTPQRDFFGVNFSEFMLQLFNEILKISKNKNIDGILFLQLIQDSLNYIQQSCKEAQDYLIFLHNIKLIASQIKDLAQSVYQNSKKQKSFQEFAEPIQQQIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XPE3</accession>
    <name>A0A8S1XPE3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1320118</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000132">
        <property type="protein sequence ID" value="CAD8202930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XPE3"/>
    <dbReference type="OrthoDB" id="6105938at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="156"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8202930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="367" mass="43683" checksum="51591960536EE9E5" modified="2022-10-12" version="1">MLPNLRNLQKRRPTAITPDTSQKRMLLQKSEHHDIYSLKSQIESLQKQLEYVQTNKSFVMKITSPPSCTQCSKFKQQLDLQQLNEKITKRNYDQKIDELHKQSQKVILTKGKTIETDSRYDDLLFKLKQIYQYELLFAEDNTININNIQKIQFSYRNSKEQNQQSIQQLLLQLEEQDQIIKKQSISIKNYNSQIDQLNNVISNLQNQLDNLKKQLKDRQELNKKTLQTLPNEVSPKLNIDSQQDKSILQQLLFQNQSLKEKLIQQNHQIDKSAQENQIQKHLLRIEVLETELKLSLRCCRCQNLFEKPRTYIPCGHSCCEKCYKYKCDSCDTEAVYRNRQFDELIRVYYIIKDTKTFIQECLLRLQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XQD2</accession>
    <name>A0A8S1XQD2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T1330111</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000133">
        <property type="protein sequence ID" value="CAD8203263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XQD2"/>
    <dbReference type="OrthoDB" id="354826at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05578">
        <property type="entry name" value="STKc_Yank1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000454">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000854">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF374">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="24"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="11">
        <location>
            <begin position="283"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="351"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="351"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="364"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8203263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="386" mass="44472" checksum="310C70B770F3D258" modified="2022-10-12" version="1">MGNCNINEKKEDLMENNVVSVQNFQFIDAIGRGGFGKVWKVRQKKNKQFYALKVMSKPKIIQKKSVQSVMNEKTLLSGLKHNFLINMQYSFQDREYLYLVMDLLSGGDLRYHIGRHRKFNEEQTKFFSGCIIVALDYLHQQGVLHRDLKPENLVFDSNGYLRLTDLGIARIWKPENSQDTSGTPGYMAPEVMCRHNHGVAVDYFALGVIIYECMLGRRPYLGRSRQEIREQMLAKQVVIKRQEIPPGWSLEAADFTNKLLQRKPQNRLGNNGPDEVKEHPWFKDFSWEKLIAKQIIAPFIPNGNEDNYLPSDSRRDSDDSINEEQQIMLRRNSVQNLFNGYDFDNTPVSVPSSQMVVSSTSSSRITKQPTTTNTPRSAKLAQKLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XSQ6</accession>
    <name>A0A8S1XSQ6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">PA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T1360031</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000136">
        <property type="protein sequence ID" value="CAD8204127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XSQ6"/>
    <dbReference type="OrthoDB" id="10045365at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056858">
        <property type="entry name" value="VSR_TRX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22702">
        <property type="entry name" value="PROTEASE-ASSOCIATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22702:SF1">
        <property type="entry name" value="PROTEASE-ASSOCIATED DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25011">
        <property type="entry name" value="VSR_TRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="383"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="10">
        <location>
            <begin position="56"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar sorting receptor thioredoxin-like" evidence="11">
        <location>
            <begin position="180"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF25011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8204127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="437" mass="50438" checksum="326A68932AC843D1" modified="2022-10-12" version="1">MLIFSILSYLVTAKFQIYMDGQLRILNYSLANFGHIPYGRTLGGFLVAPQELEDNQLDLCNKSNIQKLPDLESVWVVGRIGNCSATTKAYVAQELGAKLLIIISKKANLTNVMELNNDGMGFKVHIPTIEIAKQDGELLLQEITITSESRQYGLISFNDSKKVEKPEVILFISLNDKSGFKFIREFQQYYKILEKQVQFYISYEVEVSKRDKRMNFTQPNEQCMGGGRYCMQIRGEGLGRQVIEEQLRQHCIWINNATQWFEYMDYFDKNCFQVQHYAACSNENWIMKQKVIGECVENSYEKDSKKARELKENIIMDQWVGNKSLSGITYFPGVLVNGKPYHGNLEAESVTEDICSNMLDQTVCLALQQQEDDSPSNGSEINWAMIAFAVVSCILFGLFLFMCYRRRLKMQMQQELGQQVNEMVSQYIKFYETKNEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XT92</accession>
    <name>A0A8S1XT92_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1360084</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000136">
        <property type="protein sequence ID" value="CAD8204193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XT92"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8204193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="305" mass="35606" checksum="FFEEDCB0B23532C9" modified="2022-10-12" version="1">MNRSRRQELLQLVNKKRSFLSPSIGKSTNNKPFRRQSCRCSECGGVGPFQERQNAQHPQTYIRAHHCKKRKRRIQRKKGTYLTSTSLNVGQSENNTIILQNIPKLRKAKTISITKELQQTMRKGKRLLTKDDQQIKYEFFYNNELKLSKGFLENSLNSQTYENALISFINFTKSPSFLLNSYNNLINESKTSSDEEQTQKLLQDKQLQLTTPKIKTKNNSMHNRKITDGRMHPNINKTVQQFVQSCTTIRIMNSPKQSKQLKLPSLNSKREQNKYSVYLLSSPRKAMMELKSQIISQKKIKVFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XTP0</accession>
    <name>A0A8S1XTP0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trichocyst matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1370073</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000137">
        <property type="protein sequence ID" value="CAD8204447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XTP0"/>
    <dbReference type="OrthoDB" id="298080at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035794503" description="Trichocyst matrix protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="108"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="223"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8204447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="358" mass="40830" checksum="BF0FA863D0A6A39A" modified="2022-10-12" version="1">MYKLIALALIVSSVFAVQKDTKTILAEIDADNFGNTILSTVQMYLQSKGNAEEILVLLNQVLAGLVDDQNKHDNVIRVDRAACTRIVTDLENSIAYHTAQVSANAQMREDNEKALAEAETDVRQTIQDIESNERTYAQEEANRNKAHETWVRKNGEHDDAIAAVDEATKLVQHLSLGATFAELKPKFEAVQKRLIENESHGALFQPIVTALTELATKVDQKAIQRILQLLSQLRQQLVEARSVLEDTENRQAQRWVEFSTHLSNEHNRLVDRKNQLEQAIQTFKTNIDTATHFYEVHQLELEQAQETLDAEHEWCDLQENTYSVQSTERTRQQEIVERILEHLTEKLTATSQYLGGRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y2S6</accession>
    <name>A0A8S1Y2S6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1420093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000142">
        <property type="protein sequence ID" value="CAD8205974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y2S6"/>
    <dbReference type="OrthoDB" id="311333at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="174"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8205974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="441" mass="52874" checksum="496253E8294C2131" modified="2022-10-12" version="1">MLQVDTQERYKLTIAQFQRKMMVFNDKIIKFIGFQLQNNNQRAYDFFQVYFQEGGDQKLYSFQLKRYNNQQYEKILNENSQNFIVLQYVEDGLFLNQLERVSFLTFTEILQQLSQAIFQLHQNKVLARCISSNNIFIDRQVNKIYLFEFGFIPNANFQQVYKANYDIQLVINIMKDLYLRKQSENNQFEDLMRNIRNLEIQLQDLDEIQSYLQFYSLIQGGPSLNQLQNLYTSEWVQTMNQLIKNQDFLSPLPFDIQDPDQTDDDCQHPTQLKLQKLATKIFSNKYPTHYDQIGFMLRFQSVLNDQSIKFNLDEKLINKRRNKICENRIKFCQVNREKIDEKLLPHIKPEENEQLGDILKIYKSQTYKTYRELFKDKVLKVQETGKPDLTLDEAKICFLLIASLQQFDFQNVYQIYQSEEIVDKIEKIKGILEKYNPQIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y2U6</accession>
    <name>A0A8S1Y2U6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ABC3 transporter permease C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1420116</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000142">
        <property type="protein sequence ID" value="CAD8206004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y2U6"/>
    <dbReference type="OrthoDB" id="313105at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32522">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32522:SF3">
        <property type="entry name" value="ABC3 TRANSPORTER PERMEASE PROTEIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="419"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="456"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="479"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="518"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="576"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="600"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="629"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="666"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="940"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="987"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1031"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="domain" description="ABC3 transporter permease C-terminal" evidence="7">
        <location>
            <begin position="426"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="ABC3 transporter permease C-terminal" evidence="7">
        <location>
            <begin position="944"/>
            <end position="1062"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8206004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1070" mass="121183" checksum="A9D44E1609CBC6B2" modified="2022-10-12" version="1">MQPKPIKRKDSMVPQGTSMSICSSLLFMIGQSWYEMKRRPCGYCVSFFSVLIVVAASAVSQSIIDRAPLIFLKSAEGPAGQSDIVLMANPNYLSEEHEKSPQYLMSIYSNSARQQGYLIVDFLNFTRVKEVLGPDRANQASPRIEQQVNFTYLQKTGECENSVNKAQQLLSKRQEFGSEAHFNDLRDGQQLGQRCGGVQPHPNAKFVAIDTQKEKKIGLGWEYPFDELKETEVIMSRKLAETYKLNVGDYMLIYGEFYDFYASYYIENYYEQIGKQFNLTNYNDTFINQEFSDLKKATQRFYLYQVKGLLDSTYGKFPDGDADKLIIVEHKHLFNNLAYWSWDKPFLKAMYTANPEDFVDEIRINIPDRFNIYMDSNYENIQGKITKYASNIRRDLGVYPCNMDLPVLQQLYDSRFGQLFLGIILNMIIVILFLLSVLLLYTLLLISVETKTYDLGVLRVLGFNKLGVVFIVLTQALSYVLPAMVAGIILSIPFLLYASSALKASIGVEIEVTPTTNAIFMSLGLGLLIPLLSSYVPIKEALSKQLSFALDINRSKSTAVKIEVDLEGKSLPWGRIQFAIIASLFGICVYYFLPLALLSFNIGLLLSIFFFILCGMLLGLVIFAFNIQYLAERFTVYIFLFWASSAKKTMVLKNLAAHRIKNRRTALMYALSIAFVIFIFVGMSVQIENQATQTLQQHGCKLEITSSNGYLSPFEFENIILELGGNITSFAWVTDSLDSYMQRLGFSTAYMTHLGQVYQLRPKIVGVSHTLFDMGYNQFLKVEDQINSKLNPVEQLYTSRGSQSAIIGTSYADQLNIKIDIDSTFLLIVYNNTFSQYHQMRASSIITSAPALKFSSLPSVQEQNVVVSLPTFRRLCGNLIDAVDNYPMKRLLISVSNSGNIDTVYGYFKKYIDSKGVSAKIWDYRDYETSIKQSQDLIQIIFSFLTGVVMVLSFFSLMTSMTANMLEQVKEISVLRAIGNTKMSITMVYIMEAFTLVFSSSFIGLMVGFIIGQSMALQQTLFTQLPLIFVFPWQMLIIIIIIAMIAAIISVVTPSLQILSKEIAQIMRMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y418</accession>
    <name>A0A8S1Y418_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1500093</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000150">
        <property type="protein sequence ID" value="CAD8208340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y418"/>
    <dbReference type="OrthoDB" id="2158884at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07830">
        <property type="entry name" value="STKc_MAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001171">
        <property type="entry name" value="CMGC family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000545">
        <property type="entry name" value="CMGC family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="4"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="383"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="383"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="409"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8208340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="433" mass="50599" checksum="BF670D9B8BC3E745" modified="2022-10-12" version="1">MKKFKELKKTGDGTFGVVIKAEDTQTHDLVAIKKMKQKYHNFDECTNLREVKALLKLQNHPNIVKLKEIFLDNDTLCLVFEFVEKSIYQMYTQQKEMGKIISEDQIKSIIYQVANGLSYMHKHGYFHRDLKPENMLLTNNGIVKIIDLGCAREIRSRPPYTDYIATRWYRAPEILLKQANYNSPVDIFALGCIMAELFLNRPLFQGNSELEQFNKILSTLGTFTQQEWPEGCRLVSQMGLALAQFQPLQLQQLIPNASTEAINLLTQMIRWDPNKRITAAQMLTHPFFYNIEKIAPSLIFEEQNKQKDELKFFESHHKAKSYSQKDEFQTQFKQKQQLQQKIKDDDSNDLDDILDFITTENKPMPSKLTTQSAKTLDFNEYIPSSQTLNRQPRNLQSSQLQEQNKKENNSIYDFSHLQSFKPNKQPNLNSKYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y4B0</accession>
    <name>A0A8S1Y4B0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosomal protein S17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1450011</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000145">
        <property type="protein sequence ID" value="CAD8206704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y4B0"/>
    <dbReference type="OrthoDB" id="274752at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00364">
        <property type="entry name" value="Ribosomal_uS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_uS17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US17M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8206704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="129" mass="14960" checksum="8DB0090E661E71C9" modified="2022-10-12" version="1">MQTVWTLIHPTRISVQKTIKNLLPSGQEFFGTVIRDSRNKSCKVKVSYKYWHKKYKSYFGNSSEFIAHDEDNFCKVGDKVVIKHCQKTGGKYYYVRNVVIPVGRNPSDVQDDVMKELQNIRTQLVQSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YBX5</accession>
    <name>A0A8S1YBX5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1620116</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000162">
        <property type="protein sequence ID" value="CAD8211250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YBX5"/>
    <dbReference type="OrthoDB" id="10629641at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8211250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="21312" checksum="9F0C45BB297F81C7" modified="2022-10-12" version="1">MYFVNELSMYNFLIYFKFVLNNNLTSDINNLNHILIQLYKHFLLFSFQIDNFTTFLILLICIQFLSLIGKGLFHQFKQTTTNSSTIIIQESPVLQIDFLQEVLVNHPYILKGEYVFVKYKQFLFLQYLVINQKRKKSQNQQTFDEFKSLITSLQRRLIDSFRETEIATNALQEKSYMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YCG8</accession>
    <name>A0A8S1YCG8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1670003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000167">
        <property type="protein sequence ID" value="CAD8212166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YCG8"/>
    <dbReference type="OrthoDB" id="325695at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="226"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8212166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="292" mass="33870" checksum="FA19B97A86BDBAAE" modified="2022-10-12" version="1">MLFNCFNARLLFRVNLILMNWILLEVAIQVNLKLMFNEWIFHLIAQVAQIIMHLTYVSQVYIEFKHNVLLINLNTKFLIQLHVFHTLLLLIKDLAQLAFLTFPYLAQKVQLTLIIMNKLQIEAENKQVYSVLPLISCFQFLYKICEICVDHYLLIGTACKICDASCKTRELNNLISWQILLEFTVFSMFFLNILTLLLVILLALTQIKRQSIVMQLSSNIPPMGQINANFVLLHAQSANIMLIIVQIAFLLLHQVRVYNLNEIAASAIFLLILKHVSNLQVYVKLVNRIKTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YEE6</accession>
    <name>A0A8S1YEE6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1560076</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000156">
        <property type="protein sequence ID" value="CAD8209854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YEE6"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000586">
        <property type="entry name" value="Rab family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8209854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="253" mass="29270" checksum="43F74061D76CBE88" modified="2022-10-12" version="1">MLQNNIDENEASLLFKFILIGDVAVGKTSLLKQFVEKRFCDDYNLTIGVEFNIKYIQVNDHVIKLQMWDTSGQENFKSVTRLYYRAAAGAIMVFDITRRETFENIRNWHREALDNGNSRMKFLLIGNKTDLQNHREVESYEAAQYAKENECLYTECSALQGSNVENAFMELAHQIYQMAMEDPNIVNEQYGIKYVNNANSSMSTSKIVSQNAMPKKQLQKINNPNTEEFIEYYGEQNQNNQKKAQCNNCCADI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YEW6</accession>
    <name>A0A8S1YEW6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1590036</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000159">
        <property type="protein sequence ID" value="CAD8210392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="302862at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1699"/>
            <end position="1717"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8210392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1751" mass="204795" checksum="6F9B1C6E0CD5AD8A" modified="2022-10-12" version="1">MMFAFFCLTSQVLAYLSLELGQTYNDAIIPWQGEQNYKYYQIDIKNAKINKDLIIVVKQQSTIGNPDIYISSLDQKPNKNYSEFQCNSQGMDICVIPQPENKTYYLAVYCEDYCRYNLKVVYQEELIMTNSDDLEFKFNNTSWTEIIRININQLKQYPIKQNQNLEVSIQVKNVEILQESFQSYMNLGMQRPTPQICNFKGLDTFLGIQKFKINNVKANQTYTLLIEAQSGAIMYIKTRTYGSTKFINVGESIEDVIQENDYAFYVLNVTADQELFYLGKLILNIQLISFKGYTEMYVNLDENPASLEDYHWKLHDGVTDDLIITNEDLNRLNTKGYYVYIAILAKQSLATFELKTQMLNPDLMVIELNKPVFSKMSKSKIHQYKFYIKSNKKQSVSVSLRNIRGDADIIVKSCNEFWTCNFNEEEQKQIISQNFTNNITDNQYYYSFNPGNDIIIFDYLPVICKNYDNVHMCFYSILIIPGNNNVEDELTYSILITTKQDTIILKENTPIKQFVFHEFYNYYKFTVNDNDKIQRLFIQLTPIQGSPKIYSSKTDIYPTEDYNDRQGVQNLITYGDQTDLETINGTIYIGVYGETASQYIITAIVFRKQEDWGTIGKYAHQYIQLIEGQPQEIVTVYKYDVQLFKIDLSGYNNENSLTHNQIKIFLRINSGQFMIYAFDHPETDLKKAIQTGSKYLIINNEFLNNPQYLFIRVETNQSTTYPLYSYSINFRVSSQPIELVIGDNYQGFIEKSDKQTFFVNFYKKEDIYINFHVQNIDETILQATIYIEDRIMIMKDQNMILSANQIKYENCKKTEDIVSCQIVIEVQSREDTQFSLLISKESSIIKLYNEEPITKTITNQFDFFTFLLTEETEIAIFSLTANIRILANIKQLNPPNLEEFPTNDDDSLFQSLNDKIEMESSIFISLDDIEKSNCYNTPCYVAVTCTTADPLNKDNGEYTIIRQSGAVKLFEKFIYSGKINQNQIKYFKVVDVKESTGLQIFITSSEMNKLTILASINQMPTQQSYDFSSSFNFGDYLLIPPNIDSDQIVTYYIGVFAFQDIKVSIYVKMGLARFYPIFIQKPLRVHLPSNSTTFLQFNCGSYETLRILISSNSLNEYIKPKIHIKSYQRNELSSVLNTIQNEYHWQMEDYYLEITNTSENYCENCQYMIYLENNYQDILLTLTILKKDSLIQLQNNLEIKNLLKFNQFDRYLYAPKDQGNFYLNVTVFKGKIYLYEKKDDSDQEYSNLRLILEENDGTLDHLNYQVASREGINANRSLRISNLKENYNLSVYEFKVFSNFTSQTIYQIEIIDQNQAIELKIGQPQQLKINQHQQAQLYFIIPNRIDNYTDDYYSITIEITSLLHDRIIRAPAFTLKQDRYNNPDIQSNYHDFFNAQISVLQELDTITYIQIPSIAGLYFLSINPQEISNQQYFIMLGNKDFNILTPKSYRLIRTKVGEQQIQEIHIKESSKLFVQAQLCGGIVNIFGSSSRDNLIMGYYENKIDSIQNKQLIGTISIVQPNIYYLNTKTIKSTTQSDFVSYILRFDIIKEDTIVPIDHFYPGDGGEFQISLIENQLYFYFSPIQSSEKSSQNYVLESITYTFIYQQQNLSDNSSLDKCNQNSQYQQFTIRRSQHDPQQTLTQRANYEGDNYEKILATIQATVNIKTKNYEKLKLSYFYNTQILEAKIVTNNFFDEISRGLFTILFTLLLFILFLLLIKYRNFKQMIKFEAQTQVSSRQQEQQIEMNYTNLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YFW7</accession>
    <name>A0A8S1YFW7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1710049</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000171">
        <property type="protein sequence ID" value="CAD8212830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YFW7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8212830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="106" mass="12280" checksum="E42A04E05FDF0BBA" modified="2022-10-12" version="1">MVPYLKNSKAVYKLILTKLKAHVFGIKWLQQNIMRSAQPAQIFAFKFATDLIGSQKIELLLSKSLFESYIKLSCHQTILELKNAIIYHISKCVIRILQLQFNLRNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YGJ7</accession>
    <name>A0A8S1YGJ7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1590121</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Organophosphate:Pi antiporter (OPA) (TC 2.A.1.4) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000159">
        <property type="protein sequence ID" value="CAD8210544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YGJ7"/>
    <dbReference type="OrthoDB" id="308680at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000849">
        <property type="entry name" value="Sugar_P_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43184">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY TRANSPORTER 16, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43184:SF12">
        <property type="entry name" value="SUGAR PHOSPHATE EXCHANGER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002808">
        <property type="entry name" value="Hexose_phosphate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="17"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="233"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="262"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="288"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="329"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="358"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8210544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="395" mass="44096" checksum="A64B548CEF319F77" modified="2022-10-12" version="1">MGQPYLGQFGDRMNLRIFILAGTFTTSVIFTIIGVMIQIQDKKNYLYLILQIINGISQSTAWPGLLAILNNWFVTDKKIVLLGFFAAGPNVGDIFGDIYSGSLIGRDHLPLYAPVYLAAVSLFIISFLNAFFLQNAPSVDIKRLMIEEYEKNKANQLNVDNQQLTIAMIEQKFEPNLNTKLDDNKNEIVVIVKNLLRKRIIKLFYCLVCTKCCFLCIIIWMCQSYSKNVPDVAWITAQTDVGSIPGGILVCLIGFYFNKRAVIIVPSLWIGTIIMISINYSGSLKHEIAGYISLVFLTGLFIGGCYNNISAALTVELSNQPELKKNKQATTTVVSVIMGFGAMFAAINQLIVPYVESYLFLYCGGVSIIGGIILIPLVIREIQRMRLNSLKKSFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YGW4</accession>
    <name>A0A8S1YGW4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1620041</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000162">
        <property type="protein sequence ID" value="CAD8211102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YGW4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8211102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="42" mass="5332" checksum="794FD423C18DC9B0" modified="2022-10-12" version="1">MIDIMQDSDYLGYHYETEFQMDRILIKIKRRDQKVKEQFRYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YHA0</accession>
    <name>A0A8S1YHA0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1900001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000190">
        <property type="protein sequence ID" value="CAD8213916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YHA0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="87" mass="10570" checksum="E20C04C9D5FAE042" modified="2022-10-12" version="1">MKRKIENSIQNSQIKEEQQVQNHRLHRIMLGSLEELKRHQTKYKERKELVIQELLDVGKDHFRQDIFSANYKKSIVNRKIWANDELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YHD1</accession>
    <name>A0A8S1YHD1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1910014</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000191">
        <property type="protein sequence ID" value="CAD8213956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="28293at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002895">
        <property type="entry name" value="Paramecium_SA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01508">
        <property type="entry name" value="Paramecium_SA"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00639">
        <property type="entry name" value="PSA"/>
        <property type="match status" value="19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2075"/>
            <end position="2100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2120"/>
            <end position="2139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2175"/>
            <end position="2196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2239"/>
            <end position="2265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2277"/>
            <end position="2299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2327"/>
            <end position="2345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2351"/>
            <end position="2370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2382"/>
            <end position="2402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2414"/>
            <end position="2435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8213956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2466" mass="282911" checksum="C8A7C47AB88C03C0" modified="2022-10-12" version="1">MLEVKMNVKALVFVLGRMICAFQIPVNHTIKRQIIRIHARITHKKIVENKSSVAFILVNVLISQIATYLIKIIVKNRNINVYLMVQSVFRCQNVVIIKQRMDVRIKIKRVIIAFGLWVQRKNLPIYLKSHSMCNEGLNGCTISEQGYGCIEQMESCSQYVNYFQCFESKLRKDNCFWDTKNAKCVEKVCENIPFTQDYECKSYLSDCTTNGIHCVRRKQCSDAQNKLGCISDAQGNKCEYHENQCKNKSCDTAPNSLSNYQQCQDYDNLLDCVTSQIKGCKNRPVTCDGYAFEIDCYSIQKQDCVWYENKCEQRQCYHAPIYYSHIDCHQYGNCIGKLNGGCQITPQSCEDILQEQFCELNYNKEKCIWLGGKCVLLECNKLKLPYYKNHKICQQASQFCTFNLDSVGCTDYLCENVSEIEYCKIDSSGTICTLNQGCIQKQCKTAPPSYDTNQKCEGWMPKCTVNVQFVQNQKILIGCVDKKISCQLAIQDQCYTTISGFKCKWDGSSQSCIDQECTDADPKVYLTNDDCQQFQVFIGPCIIGSSGIGCTKWPTNCNQMINQQQCKLNLKDGTKCFWTGTYCKKQQCSDAPPINYTNNIECNTWFNNCIFNHSQGGCKDRPISLACTSSPNNNMYDTHQECFAWNPKCTVTSSYSVEGCEQKKDSCYEFIRQRNCKTTLNGQICYWDDKLQMCMNEDENNNGVADCDKRLYGDLTHQDCEEFMPKCTLNNIVRSCQYLSYYCDYQYKQQCQITRYQQPCKWDDLNQRCKNVVCTDNTTAQTEVECLKFKQQNECQLKIKSNGTYGIGCENRPTTCNSITDPVICKLTLTTNQHRCYYFNSICQVVQSNQCEVITESKSDEFCQLYNTQCVLQSSGQGCYSMFNCSDLSNKICNSAIMRYNQKCSFQDICRWDNDCSQKYLSSSHCNDQKTQFGQLCQYIYQCPWYCNYYCRLQTTQKNLTFSSSATLLDKYKQCQVYSSSYRYDTNCNCCVILTSCNLQIGSEPLCNLSIDQSRRQCGYNQQTQTCEDRKCEHLTSSQPISRQLCFEWKYNCVYDATGCKTFSGDCTTIGFIQQCYSLSCQWQDGKCVNHVNCELNTTAVTNRECLLINSSFCRLNYNKGQGCAFTNCSQITSSTICNSAQLANGERCRWDNYYSKCYNKYCQEYAIQSDCESSYGYISPIATKCYWCSIYYTKCTNNSYCNSSSMTLYKSHQDCNSVNVLTTIQFIISAKCTVKLQRCQDYIYQEACKKTIDGLDCYWYSNACLNICEAAFYLISSHTNSSCHTWNASCMQLNSSECQILDCNLLTTSANCAIFTEKCFWDGSNCQSIGDCSKYSTNTLCLNTSNSQDIPCFWNGTLCLEKTCSNKPTLSLNQSECDSWLVNCQWNVNNNQCVQDCTQANISNNTHQLCEAYYLNKSCTVKLDLIQCVDLPFSCPLAKKTQCYIDQSGNECYFQVSSGQCVNLLCSNLPSSYKKHETCNQRLKSCTVNTTLNGCQQLTDCSNYLIQDQCQIDSNKIECEWIISENKCTIKQCSTAQLPIYSAHSCQQYFGDSCTVNANQDGCETGQALCMKYTYNQCKSDGQMNLSGVDCFWNEEKSICLERICENGPPLAQSHSDCVGFLSTCQKGGCRIKGCFDYNYAIDTACASIFEDKRCVTNGYQCVLRNNCEDVKIIDGCTFDINLNPCVWINEKCQIKTCGTASVSLNKYLECNEYFPNCTVKQGGGCTKKQTCENYQIKEACFTDSENIECIWDDYLNQCFSNQCMDFCGDGVVSSKEEECDDGNYLPYDGCYKCQVQCPQGCNVCNGRICEDCQKKGWLLINGVCTSKCGDGYTVGSEQCDDGNYIEFDGCYQCSFSCHQKCLNCFQGLCLQCEFGYLEDGSQCHNICGDGNLIQQVEQCDDGNQQNNDGCSNTCEVEKNWKCQQENNISQCKYLILPMITLTKLTKTNSDNQEFQLSFSEKVRLNVNGISEEQFLQMIVVVLENANHNEYDVEIKPIISITTQLTDVAYKILVYFKSKIQNPLLKVTILCENIVNIQDNSLYSNEVKLVLKSPNKMSNDQKSLIQKTAFLSRIVMYIILIVSGIAFLFGNLEILWNLLDMLQQLSYMKFHNLQFPENLKIYFEIFSIGSFTPIINFFQTDIYLQNLFDYQIPIIPAKWKFEEYEINCYFLNNFQTLIILIIIGFAYFLVSFFFYKFLILIKYQNWPTIFYKSKSQSLFKFVRFIFSFQKLARKQCSYFVYSGLIRILTSNYYELTFASILQIANYNIDTTLNTTILLIALITLQFNLILIVLFFSYLSKKDKVSKKLSVLVEGINNHFSQGSKQYFTILLIKKTLFLFNLIVLQKLFGAQSLITACLSGVFSCYIYIYKPFENNYENLKIFITEIMIMLNVILFSVYDIIKFNQNKDSAEVLGWINVGGFTLILIFTLLIDIYQQLQKYIRQFRKNFINQKKNMKSKNFIFFAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YJI0</accession>
    <name>A0A8S1YJI0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD domain, G-beta repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1650004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000165">
        <property type="protein sequence ID" value="CAD8211722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YJI0"/>
    <dbReference type="OrthoDB" id="10255582at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="213"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="258"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8211722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="477" mass="55999" checksum="B6D4BB6E6691750F" modified="2022-10-12" version="1">MLWTCNSHIKDIEFICLNPNCSETKISCFQCIQNGNHISHLKDVKKIENFYELYSQESNDNTILLNKFGLLIEKIKHQFQIIIDGIQLKYHITESQYQNLNTEQINHLAFSFIEWKKDRDFIENTISGSINQLLESINICINTLKLQQNKINSLNIQQEQKILKYELINSIKDDDIFAFAFDNVSSLMVAGYSSSTIKVFEFNQGNLNFLQELNDHNSFIYCLYFMKKSQCFLSGSGDKSIILWSINQNKQFFCKQKLQGHNDSINCLIMSENEELIISGSSDKTIRFWTKNSQPSWNCQQILSVHKNPVNSISLNESSTQLISCAQNENFIIISQQQVDRLFWEVISTIQVDQWGYRLCYINNLMFTFQPKSKEQMYIYQIDDKNGQFKKTKELTIKAGHCSMYFPQQYIKSKSILLNKNGNHVNLIRKLENQEFVTEQSIDFRTQYIYGMMSQDGDYLITYDDQTREIQIRKIFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YKP2</accession>
    <name>A0A8S1YKP2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1950009</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000195">
        <property type="protein sequence ID" value="CAD8214078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YKP2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8214078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="259" mass="30507" checksum="5F9C3E5BFC39ABF0" modified="2022-10-12" version="1">MVFLLILFVKEFVEMERQIKLNNVMMEIFIHLMDVLIVSIHVLKVVLIVSRAFVLNVKKDGHSMNLHVLRISIQILKIKIILNSSNNVQTIVNYVQIGCVKNVILDSIQQTINVKLFVEMEQGLEMKCVNQSNRVVKIVNLFALRIVMFALKVNVINVLLDIHLIILSNIINLFVEMVLYLMMKNVMILIKKMVMDVLVNVKLNRIIFARIIKTHFQNVIMKNILHFSFHQKIHMININTYLFILIKWLKKRVRMFFQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YKY0</accession>
    <name>A0A8S1YKY0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T2220001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000222">
        <property type="protein sequence ID" value="CAD8214529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YKY0"/>
    <dbReference type="OrthoDB" id="270720at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 5-kinase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8214529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="170" mass="19952" checksum="394348BBD01A9986" modified="2022-10-12" version="1">MSKEFYGFHCEHARRYYDGGWYEGSWVYNQRHGKGQIKMDDGNEYNGDWKNDLIHGYGQYKWENGDEYEGDWVRHQMHGRGKLKMANGDEYEGDWVHHQMHGRGKLKMANGDEYEGDLENSKKNGNGQMIYADGSVYNGDWLDDKRHGFGEYESANGIKYSGQWLNDKQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YLJ3</accession>
    <name>A0A8S1YLJ3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1990003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000199">
        <property type="protein sequence ID" value="CAD8214191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YLJ3"/>
    <dbReference type="OrthoDB" id="1729737at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8214191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="320" mass="38149" checksum="4621A237D22F1E7A" modified="2022-10-12" version="1">MIFTCIEYQNENFNNTYSNKLSFLCNLQTQWLSINLLSQKFFFSFFTVSTFIQLISTYFQFQTKFKNQIRIKNQNKEKLCGQQDLQKIVSISKFSKCLLQRIAEVGNEKFSFVGDKDDINLKVIDLLENSLNYDLFDFYQQIIVFWRKIKKTNFIFKKTQNSELIVLINAQINLFDFRMNIKVLKIMNISIKQLLIGTQEMMKNQYPKKIKESQHSFVRFSKMKKSLKHQQERAHFQKKSRKDFRKEISALRIFCRQVNDGLTFTFHLKNCQKKSKISFVLQMMSSEYNKNGRNPEVLMLYPICLIKGKEEDLNNLIISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RWA8</accession>
    <name>A0A8S1RWA8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0010520</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RWA8"/>
    <dbReference type="OrthoDB" id="300278at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8132256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="360" mass="42835" checksum="E73C245B3CF5069C" modified="2022-10-12" version="1">MSEQLNASFLKQEEDIIQSKKEQILQFFDQQGYKPNTVFDDQLWFLPEINKTYDHLLWKVINDTIGYDYNKDDHFATETKIDAFVQTLLDEAKRIHIKIVELKKNIDYNKEEIDNFDKFSKIIEANKTEYNQENAFVISYVDEINQINDIAKFYTIKYKIGTILQNYQLLYPENSNRSTCITNNPQIEQITLIVMGKRLDDSIRDARNSGEDYQELAQIKIPIQDIYQQVRPRHFALPTIPQQYLIVNYQDQSLDAPYCENVTINLRFDLRLSYLYRIQIIKDQKQYRTEFIEKSEEKLQQQLGLMQQLLFPFKSEPKLHYVIAKGIGLQSDFKDDNKENQQILEEIRPKKERESCCLIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SGN8</accession>
    <name>A0A8S1SGN8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0080126</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SGN8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8139493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="157" mass="19416" checksum="EDD35C7F7F38816E" modified="2022-10-12" version="1">MLRRQSISRDFEISEISKLNALLFKRSKRNKEKLNQLLFFINQLFNDRQQFVKDNLNYQYSDNNYILKLKIFNSQKIELLNKIIRYLMFNNKNNINQKKKNNNFKQCSSCLKQKKSIYKKMKLIKNYQFWKCMKINGRRELRKKLQKLKKYNKNLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SLG6</accession>
    <name>A0A8S1SLG6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0090266</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SLG6"/>
    <dbReference type="OrthoDB" id="293541at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8140758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="313" mass="37143" checksum="058E7E92B6587850" modified="2022-10-12" version="1">MNHSDGAQSPPYFGMYPLPYWPTSSQLLLMMNFQNPLMSPIHNLGSPILTSHLSFTQPTMNYDVKQEQNNIQNQFILDSQNKETTQTKQPQQYTNQIQIIAQKLDDMNYNEITIENLEQLIHLLFAESPKLKKIQQQLKEKKCIKVVKILETLAKKIRTQSKSREELIKFCLRKAFREIFHKIQAKSTKTKLNLKAASKIFQQTYQAEKMKSISLPFRKNSKNKTMNNHFLHELFQSKQFQDQYKTFLEKLDTIIENDRNKKIKAIAEKSWEFIQSNRKTYTFKRLPWSMKNIEKLKETANEMLNYCDDQKFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SLW0</accession>
    <name>A0A8S1SLW0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0090088</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC23/SEC24 family. SEC24 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SLW0"/>
    <dbReference type="OrthoDB" id="49016at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050550">
        <property type="entry name" value="SEC23_SEC24_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803">
        <property type="entry name" value="SEC24-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803:SF4">
        <property type="entry name" value="SECRETORY 24CD, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="6">
        <location>
            <begin position="189"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="7">
        <location>
            <begin position="270"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="9">
        <location>
            <begin position="511"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="8">
        <location>
            <begin position="611"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="5">
        <location>
            <begin position="745"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="25"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8140466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="876" mass="100446" checksum="F23A20C30F4B4D26" modified="2022-10-12" version="1">MEERRLPPHLRRTQQQQPINREPEQPTQQPPVQQRQANVYQQGQNENHQQQYNAQQQYVGQQQYPQQQQYQPQQNFPTQQVEQQQRKPQKIDVDQIPRPYIKSEESITYQQFSTAPAPLYHPPCVHQFYQTFDEGNCGPRYMRSSVYAIGNEPSILQQTEIPFGIVVQPLMEPSLFESDLPQVEFTNEPLRCQRCKAYVSPYFQFCQGGNNYVCNICKMKNQVPPDYYCVLGQGNQRGDKFQRDELNKGSYEIIAPTSYIKKEIKNNLILFCIELTQISVAKGLYSQVISSLQSILDTIPYPDKTDIAFITFDSKIQFYNIPKNLTGEPQIIVVSEIDEANVPLPPEKLFLNIETDRDKIDYMLEKLSKFGETITQQANQLVSVGTVLSNAVQLMQPRSGRILYFGCSAPRYGIGKLPQKPTDTKLFGTDQEKLFYQPNDQAYVKLGQTCLRNHISVDLFIANDENFELATVSPVSNITGGSIYFYPHYNQQINGTELHYALYRNLTRSYAYDLIMTVRTSPGIILFDYYTGSGKISVRDLELSTLNSDQSIAIMLKQEEKILDPEAYIQYALLFTNQQGQRVIRVFNLQLQVVNKNSGNNAWHNIFKTGDVDCIATLLSRKNLPNIMVQSIKQIRQSLFESVINILHAYRKYCSSNSPAGQLILPEAIKILPLYLQTLSKRDILKYGNVRIDNRIFEIHLVLTQSMHFLTNFFYPKLFPVHDINSQIVADKYYVGTLTDDEKTVLPNNIATTIDKIKSDGIYILDTSQFIYIYVGQNVDQSLLLNLFGVNSFAELSSIELFTKIDTDYSTKIQNIIESLQQIRGGTYVPIRVVRQNSPQASLVQSKLVEDEQQLEKSYADYLCELHGAIQNKGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SMN4</accession>
    <name>A0A8S1SMN4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0090250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SMN4"/>
    <dbReference type="OrthoDB" id="305864at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="160"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8140726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="480" mass="57093" checksum="A700EC1817CDD950" modified="2022-10-12" version="1">MIQERYNHLSSQFEKLKHQMDQKVCLDDFVKYTSKIASEYATLQMLDFSIQSIETRMLFYSSDNQMKGSLITSRVKQQNKQTQVYEYQHTMEQQLEERLNRFRELITSQVRQFCDQVQAQDISKPFREQISNNVNNLHEKLHQLELFQIEIQRHLEAREFEKLIKKYTEVEQRLNLLENHSKGLTNLIQVLNQQKKIDEQEQKKKKKEKKMEDHLNQYLTIEDFQKYQQYIKEHYFTQVQGAQFLQRVNETEDNIKNLFNKDIYDKLSLEITDIDEKFGIVLESLQNKLNQQIQNQATKLNTFEMSLGSFMSKSEVIQMLDNPQAMMKMNSAVQNIQEKFKELYSKQIDILESSFKFTISNIQAANITENDKLNQNALLLKKCEQIKITLQEYFGQNNQQNYSQKKKQELYQQQSQQFKNQNRLESKFQNRPLSVGSRNTKLRRQSATPKVISASFSGSSAEPQFFQKVLQNPLVQKIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SNF2</accession>
    <name>A0A8S1SNF2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Trichocyst matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0120014</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SNF2"/>
    <dbReference type="OrthoDB" id="297707at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035816815" description="Trichocyst matrix protein" evidence="3">
        <location>
            <begin position="16"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="307"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="83"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="308"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8143051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="382" mass="42956" checksum="6DC43862990541BA" modified="2022-10-12" version="1">MKSIVLLVLVATAFATNLAAFEKLEQSKLGKTLLNTIAIQMQTGEPLERIFSTLYDLEERYQNDQREDDAENKAFQGVCDADLAGLNQEIANLEQRDTELQAKLDELQPVHDQKVGQRVAKLAEKALLQKVIDEAQAKRQEQNDDFEAQREEFTFVSGVLAEARRLFTDNLQAPAFLQKGSSEAVHYTPQVFAQVATHLNAAAHKAGQMKHIRTFGKAIKLMASLAVKSQQYANQELTGRIIQLIDDLQNQLSQAFDLARKTEDARRQAYEAYNTLLNRDMNKLNSAIANLEAEIQSLADQLAATHSSQDDNAARLEAKTQQRDDRRAECQQAAYEYQQRRTARDGDRQTVSDLIGHLNSNMRDLKEYIAMRVAAGDSDLSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SPI3</accession>
    <name>A0A8S1SPI3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPENT_87.1.T0100329</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SPI3"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000315">
        <property type="entry name" value="Calcium-dependent protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000771">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="10"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8141830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="480" mass="56816" checksum="0EC34B53213DB9AC" modified="2022-10-12" version="1">MDKLIGHYRFNMNHLIGRGAYGSVFKGKTQQGQPVAVKVIDKRMINKENEKHLKNEVESMRTLNHPNIVKFLDYYETQNNIYIISEFCDGGDLRDLIKRGKQDDNLVISILQQILNGYRELQNKSIIHRDLKPANILIHQNVIKIADFGFSKRINFENDLMNSVAGTPLYMAPQTVLRQPYCSKCDIWSLGMILYELIFQKPPIKAQNVLELQEQIKLPIIVPKIDNQLLQDLIQKCLQVNEDERISWDEIYNHELFCQNDYYMQQIQNIEEEKGQQLSESQSISNQNLLEYQRLMIKNYLEIEFENNQKLYLNIINTTRSLYNFSIFVNAQQRQYQYAQQLAQYLQGCAFFHLNLIDQSILFKEFDKFILTEKGQYCLQQLQFLKQMIQEKNIQLLNSTFNQFDLNELQKFLEYAIIQITTQSAQMNHAKLCFILMSKYIIEMIKYENEYPNKHLFQLIDIAQIDILSDQELAYRINCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SSP7</accession>
    <name>A0A8S1SSP7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0100151</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SSP7"/>
    <dbReference type="OrthoDB" id="307316at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="382"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="118"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8141502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="712" mass="84221" checksum="D926A1504BA277BA" modified="2022-10-12" version="1">MEAKNKTKPKSTKQKAKLQQIMSISFENIQKKNNQPDNQNNVIQEDQDESAQQFQKISEDEYQNQLDAYHQEQNKRFSFSQPMPDLYQVQLQQQQHQYQQLLNKQQNVPPVSPKHKQSQNQNQSQQIQKQQQQEQNINESHLTNITQQSPVFIPQLPIKQVFQQQLDKSTKKSSHQHEELFQINSQLDDSQDIPQMKKMSIKSEHIKKQSRQNNSNTNNISNILDNFLSHRQKVNDQSGLYQSELFEAPQLQHFGGGQNEQSVIQQQSLLQSIHNETMEIVKDQIQQYHFDDIYQHARKDSSIQKSLVNDKDEEDLDNLLQDLYECTTKQIPKQVIESNPKIVNQQAKEIMNTVFENPPQDKQSQINKPIPYLTGSKIVSEQKNENQSPAYKNKLQDEVTKFSTQEQNKKMNIEDYFNIKQQSSSNQQQNSNKETFNYQSIQEKLKNSIQIKEAENQNVYQQQQQMNFNTKPFQISSTDRNYKIKQSFGYELTFRQPQKHLLTISQYLFKYSKLIQEPNLKRLTEIKVETAIENNKNHQILFEQYDAQLFKDFLKEKDPEFYQLCSTNFILFQQGDSNETNSAWVKCYMLSENLISYLFSLGFTVDDVNFQNILKETYLYQEKNKKDKYQLDLDAAYFSVYQSFKLIYPDFNKLLEVVKFFQNPETNLIDISTVKLVLNKMINEEKINNVFKWPDSSIKYDQLFKVLTLLPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SSZ6</accession>
    <name>A0A8S1SSZ6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0110211</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SSZ6"/>
    <dbReference type="OrthoDB" id="313524at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010926">
        <property type="entry name" value="Myosin_TH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34969">
        <property type="entry name" value="OS01G0621700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34969:SF1">
        <property type="entry name" value="TH1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06017">
        <property type="entry name" value="Myosin_TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51757">
        <property type="entry name" value="TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TH1" evidence="3">
        <location>
            <begin position="266"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="442"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="49"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8142536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="553" mass="65743" checksum="EA0FD610CB55CD19" modified="2022-10-12" version="1">MSILFEEQLQSHNKEIETSMKILLQLEEYTEQENIMQIAKLLITQSEVIKEQLINIDEVEILCKDQQEQIDNLCIHKQQLINENQKYSSQIYELQNNYNSIQKQLDSEKYHSQQLSQLESHIKTLQQELDFLNNYNTQLISENKRLNENNQQLLLIIDDHETLIQRMNNDQIQLDLLKQEIQNQIQLNNKMAQSQDLLIEQMKQKIAEYEIKLDFFQQQQQSTLQSRMSFSLVDDLVNQEDIYQKVNETIQSEFQSIIQNTDSTQINNIELIDEFSYFKKEYCGLKDNRYIKEIIKKDREALKKDNIFCFSDHIFRINIRGEKSKRIMFITESNFYFFEEHNQNIKITRQFPISKIQQIICCEFNPILCCLKIKGQNDDYLIETFKLKDFIEFLNETLNCQIQISYQQNFQIQFKNYTHPKNLNEIGNGLYDGSGKQAAFKVSQKQGFLQMQQKTLFGFQDWVEVFALMTDVGLLLFKQAGDMHPILFVPCADAIIIKNPLKCKPNCLKIKYRDCEYIFNVTSQALLEEWYQELNRQVVSQHKLQLSQSLRNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1STC3</accession>
    <name>A0A8S1STC3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0100283</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1STC3"/>
    <dbReference type="OrthoDB" id="448960at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051637">
        <property type="entry name" value="Ank_repeat_dom-contain_49"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24180">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR 2C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24180:SF45">
        <property type="entry name" value="POLY [ADP-RIBOSE] POLYMERASE TANKYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="206"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="240"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="6">
        <location>
            <begin position="403"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="301"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8141742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="498" mass="58038" checksum="F545C288ABDE19EF" modified="2022-10-12" version="1">MNFSPQLGFLAQKFQEKQQNNRITKTDLEQILETANYSLYDIQSLTQQINEDEITYNQFLSIVGVSENNHNIQTYQQNNGYEMVSEYQQKLTSEQLQNIYDDYKIALKNQDFEKTKFILNQYRDIDLVNKIDPSQRQISTYIAVQGPDDNIALQTLELLFDFGANLNYKDQLGQSILFYICRDGRIKLLDFVLSTNTVNVNDQDRYGQTPLFYAARDNRYDIVIRLIQYGIDINIVDKLSNQTALFYAANGGHVEICKVLIENGCNPNHVDSSKKNALFFARKYNRKEVIELFNNYFNKNKDDMSKNSGGNDNSKGEAPRSQQQQKRKFRDLPKQSYKLIFTDNQGNQRELNSIEFQKFQQEYPEVANLIVNADELIDDTIINQIKDDDTWEKIAKKVLGIIWKSKGAHLFHQPVDQKKYGISDYYEIVTKPMDFGTIKNKLHSNVYSSCQEFYDDVMQVFENCILYNGETSEVGQIGLNIKQEFENQLELTLLKKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SUW1</accession>
    <name>A0A8S1SUW1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0130369</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SUW1"/>
    <dbReference type="OrthoDB" id="293172at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="80"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="251"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="320"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8144725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="809" mass="95132" checksum="517195736F130742" modified="2022-10-12" version="1">MSSPVTNPLFEFQWLRNKNTYKYRAVCQELQTQLDPSQLIKYATIILKRDPSDRFEEELLLIVKATENLQFFKEMNSKQKNIDNRLHTQCCQKMTYKAFQQGETVFKQGDKGENAFIILSGSVNIYSQLDQYVVNDLSYKSRKKFENCTQQQKRTHPISVINQAKREKMKNKFSTSVGMMKKLKRQNSKQVEDEQFHSIKLLQTQHIIEEVKIEEEKDKKILEIQQQMGLEMTAGIPPSKLDIDYFFIDGVFKHKFVIELMEGQMFGELALIWNQPRSATVVAKSNLELAVLTSQDYKSLLKDAELNKIEQRIKFFQNYFFRECPRFVTLRYCYNFKKIELRKGNYLIRKGDKVTQIYLIKKGELEVVHEQEIINQVSEEQQSELASSEQQLLLAIKQTQMKPLKKMIQQRRVIILSEGEIIGDVEIMLKISVSQQTIIVKSEKAKIYGLQMNHFNRILSDQTTLKKYFYDQANMKYQHNLSLIKQVDKFHQSQYEKVKLNQQLYLLKLKNESDKQQPSNLNNIIEEVDESQLGQSKQKIMSIHDYVQEYPIKIERPIINLKQSNAFPSLKLNLSNSPLFGSMTSRIDSKNKDIEEDYELQVASLKGVLNKIKFQVNHKSSQKILKQKKASEQDFRKQVQIMQQNNDEIKKYDRKYIDLVPENQKIKIKRQYEASEMIRQEMGSQSQHLKKFIEYQLMKTEMSQRISKSIEQSERLIGNNTPISKGQFFNCFTNSCKQTDNRILTESIQSPSPITQSEYKTQNFSTSIFVPKQPQQQKPVFIKSEHNLFRMKPQSRLLQARYSNHSVGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SVQ7</accession>
    <name>A0A8S1SVQ7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0120175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SVQ7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="51"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="105"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="127"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="147"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8143349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="203" mass="21674" checksum="02118A86C4080927" modified="2022-10-12" version="1">MSYSQPPPAQGYGTTPPPPPAGQQGQYPPGQYPPQGQYPPPGQYPPPGQYPPQGQYPPQGQYPPQGQYPPQGQYPPQGQYPPQGQYPPPGQYPPQGQYPPPGQYPPQGQYPPQTYPQQPGYYPPGGYPQQAPGQYPQGSTTTIIEIQQQPAQKPPQQSHGGGAVVGAAIGGALLGAALADNHHHHHHGHGPNVVVIEKGRKHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T1F1</accession>
    <name>A0A8S1T1F1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0130439</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T1F1"/>
    <dbReference type="OrthoDB" id="284123at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050313">
        <property type="term" value="F:sulfur dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070813">
        <property type="term" value="P:hydrogen sulfide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07724">
        <property type="entry name" value="POD-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00158">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000030">
        <property type="entry name" value="Metallo-beta-lactamase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000110">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000148">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051682">
        <property type="entry name" value="Mito_Persulfide_Diox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044528">
        <property type="entry name" value="POD-like_MBL-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43084">
        <property type="entry name" value="PERSULFIDE DIOXYGENASE ETHE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43084:SF1">
        <property type="entry name" value="PERSULFIDE DIOXYGENASE ETHE1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="295"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="393"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8144864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="474" mass="53415" checksum="F169FC7963D02D58" modified="2022-10-12" version="1">MIKILQKFNRTLFHFSKEVQLKQSETLGKGFYQQQIFTGCLAEYAYYIESNKEAIIIDPLRDIQPYLNIAKERGAKIKYVLLTHFHADFIAGHLSLQRETDAQIIMGPKATAPFINKVLNDNEFLKLGKVKIQALHTPGHTQESTCFLLYDEEGKQHSIYTGDTLFLGEVGRPDLAVKSDITQYDLAAMLYASLKQKIFGLPDDVIVFPGHGAGSACGKNISAGYSCTVGNQKKNNYALQEMDQETFVQLVASDLPKPPQYFFYNAGLNKNNQFQDLNLILKKANTKLTPAQIVQKQGIQIIDSRNSISDGFIPGSINVPLQIPFAHWVGTLLPHEREIVIVCEPGNEEQTIMRLSRIGYDNIVGYLHFDDWKSSGNSISKPTELEVQDFLKRSKLAQIVDVRTKEEWGRGILKHAQLITLSELPQNLEKLDKLRDISIYCGTGQRAKIAHTLLVANGFTRVQYCKVGYDDIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T3M1</accession>
    <name>A0A8S1T3M1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0150182</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T3M1"/>
    <dbReference type="OrthoDB" id="354826at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000454">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000819">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF374">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="22"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="10">
        <location>
            <begin position="280"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8146116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="364" mass="41319" checksum="4D2EC6364A1FD509" modified="2022-10-12" version="1">MGGCEFKFRGSNDDTQMALQNFELISVVGQGGFGKVYKARLNKTRNLVVALKVMSKVKVIQKKSVSSVMNELQIISTLRHEFIINIISAFQDRCSLYLAMDFLAGGDLRFHLCKNRKFSEPITKHIAICIIIGLDYIHSNGIIHRDIKPENLVFDNQGYLRITDFGIARIWKPQNSHETSGTPGYMAPEVMCRQNHGVAVDYFALGIIIHECILGKRPYNGKSRQEIRDQIIAKQAAITEVPQGWSNAAIAFANALMQRKPQLRLGCNGPDEVKSHPWFKDINWKDYEKKLINSPFIPDGKLENYLKSNQLDSLEDQSILHSEQVQQQFLGYEHFPQLNGGSPYISSIVSPRTPNLRIQLKKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T579</accession>
    <name>A0A8S1T579_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0150141</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T579"/>
    <dbReference type="OrthoDB" id="311815at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8146034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="167" mass="19517" checksum="86E5A4D3E85145FC" modified="2022-10-12" version="1">MNYFPPSQKNIDTKDLDRIQQDENPTQNIVNQNQFRVQFSNCQEIQDIGESIVFQQSQFLKMINELNKNGDVIKLDMKAEEFNNIFAFMIQVKDILKGQEHQININIENIKTSEQFKDLFKDMSEKQVLPLLSLAEYLDIPNLIILLAAQYSSLIKDQPASINNWNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T7R0</accession>
    <name>A0A8S1T7R0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0170157</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T7R0"/>
    <dbReference type="OrthoDB" id="415460at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="343"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="387"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="444"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="488"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="521"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="7">
        <location>
            <begin position="627"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1090"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1099"/>
            <end position="1109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8147818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1250" mass="145999" checksum="4EE436AD6C691422" modified="2022-10-12" version="1">MINRDTGFQDLESGIQSNNISSGQLFDIDQASPIGGQQLDPTLEFSQLQQLEAVRMKRDIFDNSHKNAIHLGHKADVNEEDCLMSNLSGKRSEPQGSISSISCNLKNQKPLLRKTTINKSNKTIQNKQKLDQDDFVISSPLQINGTMNLGLKSDAKIAKYQTFVQQLEDEQEQNERTKRAQINRDTIKQQSLIQRGKSKEMKTGLNEVWTQKALIIIRLVSRFIQQLKTKTERIKFRLITQRIFEVICDNSANFEYMLINRLIKQKPSISLIIFYQIQNKALKLLNWIESINAFFGKYVKVIKPDSLFKIVWDIILLLFIVVNIFYIPIYISFDVRSQGVFEWIFDLLPSWVFIAEILLNFNTAYYDKGLMHEDRKQIMKHYVKGNFFWDIIVIIPFLISYMDIPFVRYTLLLRLTRLSPLMTSIEEVLNLEDNMQVFLDLLKLIFFLLLTGHFCGCAWHWVAVIEYENYGEELTWLTKYAPDAMDYYWFDRYIISLYWSVITTVTVGYGDIVPVTTVERVFVIVVTLLICGVFGYCLSNIGNIFKQISDKKAVYKQRIREINQHIRKRGLSYNLQLKVKKYFEYFFKVKQEEDQHAEQFIEQLTKHLREEVLVDIYSKTLTQSRFLRENFSDETIHRLCQIVKETKLYPEQLLFQRNDSPKALWFVLSGAVEYIADHQNEDEHFYTETFLKKITQGAVIGEREFISQTPYEYNARATKFTQLLVIDYQQFYLILQENSEEFEKYSMAKDNLIFNSNYKGFGQICEICGWTHRFIQCPFVFLQPNKNKIASSFASTKTNKRLIFPYRTLPKSNWKNNLPDVQEAALGYIVLNNIIPEKEINDAYLINLGFELNDRDDDPQKLIPNKKNSLFKDQKILSQFDNNLQQQPVQQPSIQTVLPADLKSQNQTAIFLKDGQRPSMTNESVIDWDQGSQFRKSLNRIKLQGLDRGKTIQSIKGKYHQNNNDVLDESLIQEEVKIQNANFGSQNYIHNKLKVVKNKLNNNSNNMGIRKVSWLEFDSGTNQHLDNKPINLLKQASKGSETNFDQSPPSCSQEIEIKQRIEARENSIISKKTSNIEVNRRKKRRRTTQLLQFFQGSEMDNKSNKKVEKSVPNNEAYSSSVYTAGVPSNSALVDSNNKFDNNGDNNSLQVGQSMDGMQKRIQDIVRVHFEMDMDNAKSSKIYFPDFNLEVVIQRINVYYEKLNEKKEEFDMREMKRTKTNFTLFDRIRQAKNTTIRGSFVDKDKSLQDND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TB12</accession>
    <name>A0A8S1TB12_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0170179</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TB12"/>
    <dbReference type="OrthoDB" id="291688at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="408"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="444"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8147862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="539" mass="62963" checksum="B379AEE40CA018C5" modified="2022-10-12" version="1">MARLIGNTNIKLRNPLLKLCQPISSISKLIALKLVFIDDISNHYTINCKKVNCFYTQGKQIKIEKLVEKELNQYSDSNQLKNPKFYFYDLNGNQIIMYDLSIETYLVSTKDNNHWLYDKKYEKLLFQIIQMDASIISNNNLSDDKSRKPSLMNSNQHIIHNNLYDHLIPQSSFRSRILQKGIQDLENVYDNFKMTSRQNSDPFIITNCKFKTTHQSFSSQNRPSNVSYIKTNIPNSTPRHYLTDQEQYFIDNMQKCQDELDNFINKLKTNLSKENEEVEKIIKFYENVLYPTPKSRALEMKQISTSSLISTKLTKILGKQFPTDTKAPKAYVPQEKKMLHKIPPLLHLNIPSLQAKYRLNRSQLYAFFSLYKVLHLISGYMKIKSKQLKVQGITYEVYRNGIETIQDQAENMARGIFDIIDSRCSGFLDWGQFLYLMSSVQAKTRDQRIDLFIKIADSNKDGQLSYQEVVRLSQQTLQKFIKNSNREFLDEMSQFFSKIIFDSVGVDYQQEIQFNQLKEVINQGHPNSDLLCMFCGADS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TBY4</accession>
    <name>A0A8S1TBY4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RING-CH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0200030</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8149980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TBY4"/>
    <dbReference type="OrthoDB" id="412381at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16495">
        <property type="entry name" value="RING_CH-C4HC3_MARCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46065">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCH 2/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46065:SF3">
        <property type="entry name" value="FI20425P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="11">
        <location>
            <begin position="85"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8149980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="303" mass="35179" checksum="FE046FF1334DCF51" modified="2022-10-12" version="1">MSTNPNMSDIRFTQQSPNRNFNPLNSSMQFIPSVNLDIQSDEYISPRKDIRKKGILKTFYYQDVKRKKLIGCNIYVNQMNEQQLQLSKRGMICRICMNEEETSRFIMPCICKGSLQYVHEECLKLWILQKNGINDVFQDKIKCELCSQKFSMRMQLHNHFDKSRFWDVPKNQKICWLIQLFFIFGIISTIVVLFNKFGLSSIGIDAVMTLLIVLCLIMIVQFIASVIEFHLIEMIEEWTICNYRPRRETKQGSIFQLQSQMGQSISGSPIAKKKTAQIHPNGPTSLQIIQVSQLATSPNISEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TFR3</accession>
    <name>A0A8S1TFR3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Tubulin-specific chaperone A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0200366</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Tubulin-folding protein; involved in the early step of the tubulin folding pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
            <location evidence="2 9">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the TBCA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TFR3"/>
    <dbReference type="OrthoDB" id="296187at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048487">
        <property type="term" value="F:beta-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007023">
        <property type="term" value="P:post-chaperonin tubulin folding pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.90:FF:000010">
        <property type="entry name" value="Tubulin-specific chaperone A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004226">
        <property type="entry name" value="TBCA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21500">
        <property type="entry name" value="TUBULIN-SPECIFIC CHAPERONE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21500:SF0">
        <property type="entry name" value="TUBULIN-SPECIFIC CHAPERONE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02970">
        <property type="entry name" value="TBCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6 9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8150603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="120" mass="13943" checksum="EA8404FC3C716D1B" modified="2022-10-12" version="1">MAENNKDLKQLKIKTAALKRIQKEFFGYQKEELKQNERIQKLKDQNADEADIKKQEEVLQETVQMYPNIIGRLVESVNELQVWLDGKINDPLIDTSEEKTKALETIVEAQEFLKANNPAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TGQ3</accession>
    <name>A0A8S1TGQ3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Acyl carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0210115</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Carrier of the growing fatty acid chain in fatty acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the acyl carrier protein (ACP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8150987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TGQ3"/>
    <dbReference type="OrthoDB" id="448946at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000035">
        <property type="term" value="F:acyl binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000036">
        <property type="term" value="F:acyl carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01217">
        <property type="entry name" value="Acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003231">
        <property type="entry name" value="ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863">
        <property type="entry name" value="ACYL CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863:SF76">
        <property type="entry name" value="CARRIER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3 8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="2 8" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="9">
        <location>
            <begin position="32"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8150987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="116" mass="13193" checksum="B893531B5463BC04" modified="2022-10-12" version="1">MFVRALRLINPMGFQCRNFRFCSTIQNKELLEEVTAKVLQVMKSAAKCKVDKLSDKATFEELGFDSLDAVEMVVALEENFGFDIQNEEAERIKGVGEAIAVFYKYVVQRNSTSVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TMA2</accession>
    <name>A0A8S1TMA2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BRCA2 OB1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0260333</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8155141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TMA2"/>
    <dbReference type="OrthoDB" id="21095at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015525">
        <property type="entry name" value="BRCA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015187">
        <property type="entry name" value="BRCA2_OB_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11289:SF0">
        <property type="entry name" value="BREAST CANCER TYPE 2 SUSCEPTIBILITY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11289">
        <property type="entry name" value="BREAST CANCER TYPE 2 SUSCEPTIBILITY PROTEIN BRCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09103">
        <property type="entry name" value="BRCA-2_OB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCA2 OB1" evidence="1">
        <location>
            <begin position="234"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8155141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="572" mass="67972" checksum="FC2DE3A0DE7BF1EA" modified="2022-10-12" version="1">MISGFQTGKNKKIQLVENDKYNKIIIQFEDLSEEEEIIKSDNKPTKNDLNLLNSLSKEAIKINNQLYHPSKFAEQEFKFMSKNPRDSLSEKKISFQTVIMADEIQKEIITPIKKKEYNNNNEIFQMNTILLSKISKLPKCLIGEKFYEISKQFMKNSLLKFSSFCRLSTIPQNFDWKLIKEYFKQYDVEKDWLYEQAKLVFWKLQSKNNYTDQKLILELEYRYFIQYRLQSRTIIQEIWTNDQSLNQHMILLVVAIEQCQGKKILELSDGWYSIFFICEQSYDQIYNKIKIGQKLHVTNLKEYPIIFNTSNVKIITQYPFNKMLVTTKINSIKKAQINSKLGKQQQKYFVRSLKSLRNGPIPCIDVFIVSKSYLIKVNQKNQRKAILFNSETENEEEMQQLRNSNLCFWITVMDTLNYFDERKQKITEFKDILVHINDPLQYEMISVGQRVQIINAIQFNSDISIKLTKSSGILFELQQYQDYFNKLQIIGNKHEIQIKVNNHIKLEQIDVIEGSNDQYQNVKIEIQNSFFGEILKDQQMKILKVTQLNKINNERFQTTQMSKLINIINYNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TQP0</accession>
    <name>A0A8S1TQP0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0250312</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8154318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TQP0"/>
    <dbReference type="OrthoDB" id="303442at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8154318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="412" mass="48205" checksum="D7FC1654AAA0D026" modified="2022-10-12" version="1">MRNRLSGSSFKNQAQQLPIHSSLTFNRKRSCECLDCGLHNKKMLYVTTVLPQFQPYQHIITKLKEQNEILQQRKRMKLRNKRHLIQKKTIIDIMQQQEHQPKKRQNRHHNTVFYNHQASIPNLSSLYSIEVAQTPSKPISRSSIFFHRYNSLSPRKLFTDKKNSKVQSFSQRLKTFYLPITAPFESQLIFRLELVEDGKIKLIKQTGSQDDFNINIRLNKFEEDDKKNEDQSTQNLANLPTLQQEDEEEDDQTDDENIVNIKQLTTQNPKQAYKNILKYNPRTLQSLVDKSQLGQSKIKDIKTACPKYLTQNHVEHCHTSITNNMSPFNNTINRCSPIPSIKLLEEKRKKPQIKLLPLEIKPFQQFTLTNISPTTLSRTKPSPKKGPQRNIFNAPKKKHQKNITFGQLLIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TSE8</accession>
    <name>A0A8S1TSE8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0250379</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8154452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TSE8"/>
    <dbReference type="OrthoDB" id="291019at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8154452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="253" mass="29786" checksum="8FA5A9B340939F68" modified="2022-10-12" version="1">MFSKKIKKQELKLIDNDKDDDQQQHPNVNQHPQIEKSIQKQKRVGINVQQLAKEKQICQKKSATNNERERNFLNQDSARGLTSINYIKQKTEKPDKLMEKYVQEKIKSVDPLAFCGQVENVQDEQQRDAELLEEIRETQAKFLSKNEEKDPSFYVGGIIQVNLSLEEEAQKLAQMEDLKRDQYIQTIIQGKPKRAAPVVVHPDEEEDKETKAFRELKSKFYQKGRIYKYQQMKKTIDNIKPEIEQSQNHENIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TXX9</accession>
    <name>A0A8S1TXX9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0300298</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000030">
        <property type="protein sequence ID" value="CAD8157695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TXX9"/>
    <dbReference type="OrthoDB" id="295893at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006615">
        <property type="entry name" value="Pept_C19_DUSP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06337">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51283">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUSP" evidence="1">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8157695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="632" mass="77089" checksum="99F15A3B1D831B32" modified="2022-10-12" version="1">MKLIEIVQLIEKFQKEQNYHDIVYIISKSWVQTFKDSVKFNCKNNIPMKPINHDIVDETKSNVLQYQDEIYNSFLKDNLKLNVDYIYINQQIWSHLNTLYDGHCIKRQLRNSNQINEFIIYNFIPVYQSQQHFIEGIQQFDSSWNISQIISHLENALQNQLSFQIPKSNFIQLYTLKNENQMKFVQIVQEIKEKKIEVIKIEELSQMRDKKYIIMDIKQLKKDFFFEKTQQINHDKSETILNQEYIEYCRQGLTIFDDQQTLLSCMLKCYEMIDVIRNEILYNSTQKYQNLKQILQESYYGFNEYIYYDLLDYEFLEEKQVVITKSRYDAIYIYNNENRVIINNQKNIRQLVESLLSHPQLQCDFNSVLFECSNEYYTEYIDFTKPAYSLKEGYKFQLRKLMEQEHNINDEDKIEIRVHQCTYENIGYQQYKPFAPILKCFISQQAKFQDLHMLIANLFEEINYENYKEFRLNKKYKLYFQTDQQGLKKCTFCQQKLCNNCQVPFNDKSLNFGSRMINIYVIYDEILNIVSNYQPQYFDNYYLREYFEVDWAKDKILILNINQEQIKPYNIKYYFDSHLYQLQGIIEKVSQNEYQCYCLKQQQWYRFTKNKFQKIDNINDDLNVVKLFYIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TYW9</accession>
    <name>A0A8S1TYW9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0300191</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000030">
        <property type="protein sequence ID" value="CAD8157490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009190">
        <property type="term" value="P:cyclic nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627:SF12">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="316"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1010"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1086"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1118"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1148"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1187"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1309"/>
            <end position="1328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1334"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1366"/>
            <end position="1384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1390"/>
            <end position="1407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1419"/>
            <end position="1435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1447"/>
            <end position="1467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1953"/>
            <end position="1976"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1988"/>
            <end position="2007"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2014"/>
            <end position="2036"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2042"/>
            <end position="2059"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2071"/>
            <end position="2088"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="9">
        <location>
            <begin position="1522"/>
            <end position="1653"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="9">
        <location>
            <begin position="2164"/>
            <end position="2295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1808"/>
            <end position="1838"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1859"/>
            <end position="1880"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1814"/>
            <end position="1829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1865"/>
            <end position="1880"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8157490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2348" mass="274269" checksum="9FB852B650B17568" modified="2022-10-12" version="1">MKWLNSLIMYLYMKQSSKVQKLILQKTENNMKKIELVNGEVKSLTGYGKSYIGNKVESFTFKQHILEVLSFLWRFEILMIMVYIIISWVLQDDAASNLVVLFQSIVLLLDYSLYVTKCLLKFRNFRIIESQLNEQRYFILENFIHSKEPLASDSSEDINTYFYPQKQVLREQIKVGDIVILQKTHECPADVLLLESSQQMVHIDQNEFGISTPKITIPCQSTFIQPKSDTKGLLVEFKLTLNGQIQYSYLFGKKSSLMGMIKLKNDPKVFKFNQDNIIFSGEKLLNTDWALGIVLVAGNNCLRIYKPSKHKRQKSLFFIGMIILGYVISLLGNLIYKSNYGIHLCFNSMLLPLYAIFYKEFVQIFNYIKQPSIQKKVNPINAIEQGNNQGKDIVQDDKHIWIEFNRVKNMTEIYSQNKLSKYDNIKDFSIMMQQTNYWKCSKVNLVCLELNQQYFQKKRKIQSIYIDDKQYTFKYKQLVAVMNNNQIQLGDNYYKTLLDTGRENDIFDEDKKDYSELINSHPYSPHNQEERQNYGISTNLQFSLSKKTIDASQQKKLKNSLGKTISFRTKVVKSGGDQSSSINQLQKQQLNEQQFITQLFDDFDNQYHERIVALLLLCEIENKYNEQKKTIETYCKNPQDYILIEFCNLLEYKLKCSIEAERQIYDIYLKTQLKIIEIQGKLKQYKILSENKQTKNRIGVYSVLLKDPEEFNEDEGALLYIKIETSQMYENTHMNINVLEINSTLYYMLKQGQQPTLYLKKQLKKSEAKQFLLNKMNIMNSYTADEDQLELLYLQLESQAELLMIIGSHAKIDQSALLTIDAFSQANLNTWLISAESQFVVLRFLFDAKFIENENDLNQFNSGDERTLKFVIQSACSNLIKQQRIFEAQDIDKKYILLSCEALLTIIKDEFLLNHFAIIVWFSRGLALYDSQEISKVKLFKIAKKLNMQIGYIGSCANTQRVYNMSQISIDINPKCYTQSKPCYLVNSLRCAESILISQCISSSLLAQQILYFWLYEMILFVLINFINQGQFLTQSCNILLLLSKTFTVMIQLISHNKRFKTSEVYYKGLIPQRCLVKALFNKDSLIKIFFVGVIDATIVSIPLYATKYLYGDSNYLPISQFAFIITLELIVKIQLYFQIFEQQYKQLLGLLLLSVFIVALVTFLTIPDYSDYKVSYSDLFYSPGYIIRSLIFVYIFAFVFKFAVTSILQLYNLEMFQDTLITDESNFKCIEQTKKLRTTKQSLQLSIQQRKKFIKSILADNNQMDDSIYKALNNNINNDEQIELKKNLTFNQKEFEIRYQESMSQNSILYIIFEATIFVNLLIQINYIEKNIAIYILWGVQLFYSITIYILQYLEITPRKIQQNLQLISILSRFLFQFITIFLLDQDYIMNQIIFTSIFMIILLGYSIKLSLKFKGTIILISLISLGVVLKFIIPKYEYFPQKQIMFVIALIIICSLSSLSGYYLCYVNEYLRRQNFLLQEQLEKEQKKLNDALGILMPRFIKERMARGYIQIQEDQGPVTILFCEICDFDDIIESEKVSIISFLDSIYRTFDTICNQYNIQKIETVGQIYVCASGLKDYDIIDKQSKNSTQKMIECAIAMMESTNNLKYQDKQIIIKIGINYGNVMAGVIGQHKPQFSLIGDTVNTTSRVCTVCEPGRISLTESAFIRVRQMKYQFQVKEINIKNIYVLYSLVINKKSTFATKVDKSPIIKLVSSIQFKKLDSNDKPLINDLMTNAVNPAGFQFDIARKSSMSRNKSVYQLPVEQFGKVIQDVITANKLLQDISNRRITMHRKSRVTRFKEVNPQMMSSHDQIQQQQQQQQQQQQQQQKKDDPLGDQFGDLFDNLKIADSQKNIDDLLSKSPQNNVKSQKSLQNNNNNHLFDSTLQQEEKIEIPKLQQLIGNQEKIETDIMQELIRVKCDFGTNQARRVYFRQFKYKEMIRIYNHKLFQDTVQYCLILFIYLMIFQLPILFALMEYKQYLGVNLTIEGLKIAFYLMSCIVVPFCLRDAYKYIFQISMIIGYFICILFTIITIIINDPIERSYITLILAYTLCLGYFLRALPYFTSRHRKLLFILKYINIIFILSFSNEWNETIIFILTIEIFILFQRNQNEDLLYQNYNIHDQLLQQQQEQNKIISHLLPSHILQQYFKEQDTIPDVFENATILYADIAGFTKYSTSVDAQQVLQMLSSLFNKFDVACTENNVYKLYSIGDSYVVLGVLDANNGIREEEAYNVVNLGFQLLDIVNLLKKNIQYPDINMRIGIHTGNIIGGIVGTDIVRYDIYGSDVALAKKMEQNSQIGRIMVSEDTMKFLDATYTGEFSYVFANQIEHRNRIIQGYYIDKVQAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U1C3</accession>
    <name>A0A8S1U1C3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0300032</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000030">
        <property type="protein sequence ID" value="CAD8157199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U1C3"/>
    <dbReference type="OrthoDB" id="294710at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000445">
        <property type="term" value="C:THO complex part of transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021861">
        <property type="entry name" value="THO_THOC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13265:SF0">
        <property type="entry name" value="HPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13265">
        <property type="entry name" value="THO COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11957">
        <property type="entry name" value="efThoc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8157199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="494" mass="58982" checksum="59AA63532C682AB9" modified="2022-10-12" version="1">MSEFKQMEIESYILNFKVRHTEIIKQKLQKLHEEESADQLQPFYLSFLKNCRDYLHQKLKLTSKKVLDEDLLELLKVNIFLAELSYSICKEVPDLKSYPLTFSEDIYECLSLSELQDYFNNLYSQISTISKFQDENKYVLLRICNSMLKRLSTTHDTFLRGQVQIFLTQIFSTIHPSLRKKPINVRDRGFDEEAVKVNASLPYSFYKNFWTLQKYLNNSELIFRNKVEFDELGEEAGQVSQGVSEPQRTKNLQAVIKVIMRALQYFKENPIEPEIITVKRFPKFLTKYSLFKNQLNDPYFRKLFLNQVLLFIFVAELENQEKQFIQNLKIEVQKQLDELGDKLGEKVQHLIDTERTWRLWVTEKQCFDDGYEKLSIKASEKAKKKRQEIQKRIYDDLKYKQTDAFEYILQDQGRPYKIETNPIEPGLNYYLNEVFYQLDQSRKIADNERCKNLKDYAWKSVRVISKFMLKELKITNLITQDLKPLPNLEDIEMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U5K6</accession>
    <name>A0A8S1U5K6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0320228</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8158927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U5K6"/>
    <dbReference type="OrthoDB" id="311946at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05471">
        <property type="entry name" value="pepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000008">
        <property type="entry name" value="Cathepsin D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000167">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034164">
        <property type="entry name" value="Pepsin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF51">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="335"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="7">
        <location>
            <begin position="14"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="32"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="45"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="245"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8158927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="360" mass="41237" checksum="791E1561C3093A23" modified="2022-10-12" version="1">MDAFKLSNYGNVQYYGPIQIGTPNQQLSVIFDTGSPYLWVPSDNCSIFKCHLSKRFQTSKSSTYKNFSKPDEVEYASGGCKGFWGSDYVQLLGDSNTRVQTNILFTYQDSGFEAVQSDGLLGLSNDKEIANIFDLAYKQGQIKSNLFAMELKSINDQSLFYYDDIPNEIMNNVEWIRVLRKDYWTVKVLNIQLNENFMSSGKIKEALIDSGTSLLYLPENVVISIYQELILNNCRLLDGNVFCPCLIPRNQVDNYPKIKFYLEGIILELDINDYMLLDFQYQGLCYIGIEIESQMNLMIFGDIVMRKYIIVFDKQSNNIGFKGAQKLNNIKTTSIFWIEIIGFGVITISCILLQMKLIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U8P3</accession>
    <name>A0A8S1U8P3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0350165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U8P3"/>
    <dbReference type="OrthoDB" id="294442at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="391"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8160774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="562" mass="67189" checksum="082781D97EAFDCB5" modified="2022-10-12" version="1">MFKLLNCLKYLSLKTATSLLQLLIISIVIGLCLIVLAVNRIMMDALISEISDKLLFKQNFQQYELQTEMLKYQVNWPMIQRFQMMNSFSKIYQNFMPQVLFQNFNYPMECPIYKGQLHEQYSTLFQLPEFCISYKNQTRQIDNRQFYPFLNFLNQLIIPFTYTPISELYMTNTDENYLFASNPPIFDYPSYNPQVRPWYINHMEQSQKSNSQGFVSYVYKSYGNNQYSFTITYSLFENNPSNKNQRGDLQAVMGYDLSFNQYADHLRFQQFVFLITNLLGQIICTNSIEDIRLDQEIYYVYQQNLTGFTEKDWQQMQYSAQRQPYINNCTLQINHLCRFNTKYQEDIILHVLNLKSDFYLIIFQNVTIQKENIEMAEKTKMEIIQAFAKNISYLVGASLALLICSWIGMYLFFRPIKQMREKMDTLIRNKFSSHNWMQKIQNEFDEKNTNYLKQALKNLKSKITNIKRKKCLNCYLIENFKYPQRILTIDFYQIKNLIKKMPQDQIKEEQISQREIKIDNIIKSPENTNIIPQIITNKLYSQSLNSEIDENVQLINSMDRIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UBH2</accession>
    <name>A0A8S1UBH2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PPENT_87.1.T0340090</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
            <location evidence="1">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the dynein heavy chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8159976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5593012at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005858">
        <property type="term" value="C:axonemal dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070286">
        <property type="term" value="P:axonemal dynein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060294">
        <property type="term" value="P:cilium movement involved in cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.30:FF:000002">
        <property type="entry name" value="Dynein axonemal heavy chain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1220:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.710:FF:000004">
        <property type="entry name" value="Dynein axonemal heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.100:FF:000004">
        <property type="entry name" value="Dynein axonemal heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002141">
        <property type="entry name" value="Dynein heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.180.20:FF:000003">
        <property type="entry name" value="Dynein heavy chain 12, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2620:FF:000002">
        <property type="entry name" value="Dynein heavy chain 2, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1120:FF:000001">
        <property type="entry name" value="dynein heavy chain 2, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.20:FF:000001">
        <property type="entry name" value="dynein heavy chain 2, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000223">
        <property type="entry name" value="Dynein heavy chain 3, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000044">
        <property type="entry name" value="Dynein heavy chain 5, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001328">
        <property type="entry name" value="Dynein heavy chain 6, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.720:FF:000001">
        <property type="entry name" value="dynein heavy chain 7, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.280:FF:000001">
        <property type="entry name" value="dynein heavy chain 7, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.490.20:FF:000001">
        <property type="entry name" value="dynein heavy chain 7, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000362">
        <property type="entry name" value="Dynein, axonemal, heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041589">
        <property type="entry name" value="DNAH3_AAA_lid_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878:SF70">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 2, AXONEMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17857">
        <property type="entry name" value="AAA_lid_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="12" id="KW-0969">Cilium</keyword>
    <keyword evidence="11 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0243">Dynein</keyword>
    <keyword evidence="9" id="KW-0282">Flagellum</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="13" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="17">
        <location>
            <begin position="1615"/>
            <end position="1754"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="17">
        <location>
            <begin position="2257"/>
            <end position="2410"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="954"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="2937"/>
            <end position="2964"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="3113"/>
            <end position="3182"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="3477"/>
            <end position="3504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAD8159976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="4326" mass="500103" checksum="46ECDB1163C01D37" modified="2022-10-12" version="1">[encoded data, 4326 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UC03</accession>
    <name>A0A8S1UC03_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Arf-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0380236</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UC03"/>
    <dbReference type="OrthoDB" id="294760at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048205">
        <property type="term" value="P:COPI coating of Golgi vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08831">
        <property type="entry name" value="ArfGap_ArfGap2_3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45686:SF4">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE ACTIVATING PROTEIN 3, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45686">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE ACTIVATING PROTEIN 3, ISOFORM H-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="5">
        <location>
            <begin position="12"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="212"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="145"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8162781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="357" mass="40696" checksum="5024C5997B5CF305" modified="2022-10-12" version="1">MENQIIDDAERDQIIKQLKLVQGNDKCIDCGKKNTKWASVTLGLFLCIDCSGKHREYGVRYTFARSLTLDSWSRKQITFLQVGGNEKALEYFQSVGLIGPGCSQIDYKSPLVEKYKQELLKQLNIIRPSLIPSPVKIAQTSEKQAQNKEEESPIKEQPKQVFQNNLLQEEAIVTKKSNKIVFADNAKPQAVSGKAVQGKKLADVDFDSLQFDDPFSNPFNNDPFKSDSNKIEQQQQDEPKVIIKQTQQPTQTIPQSNETLEKLKDKNVKSISSETLFQSQDSEQNKQNIYKFNGQTAISSKQFFGEKEEDSEDSSQKMDQFKNMFNFATEKTMETFGTVKERAGGIWENLKTKFNKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UCL2</accession>
    <name>A0A8S1UCL2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0360319</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UCL2"/>
    <dbReference type="OrthoDB" id="289047at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398:SF0">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8161703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="484" mass="56131" checksum="ECCB8009B847C8FB" modified="2022-10-12" version="1">MMMRINKTFRKLDIFGQQIRLKASGSSLYQTHIGAFMTLLLFSVLIYSGSLFILEINKGKNANFNSQDAVIQNNEGFSFNSTELIFSAGLLDILGQPIPNEGNRIFKIGFYVCNKSLNDTECIVIPGTICGDRIRATSKQLNIPQAYENITYCMSDEYIKENPEIRIQGSNRYDNFTLLGALIERCSNTTDIQNCASNEEIDKYTANSNLYYSYSFHQLNKESDDSPYQKTENIDLTSLYHKVGKYIKIYFQYSQSYLEYNPFYFFPRQVYHEGVEYQKTVIDSVLYFQDATTLARIEVHLDAKKKVCFITYQTLMDVVAKLGGLFTIIKALLNFLLQPIQSILYKLFLIRFFINHQKKGDSKDVLLRDSKLKDICIEKLITSSTMRTFFYQQSKLINKYLDVRQILINPIQFTQKIEDLQDSINKIDMLSSRQIKLISRHEVDELLVQKNNEEFINSYGSINSENKIGNQDYYVIPKLKVQMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UDN5</accession>
    <name>A0A8S1UDN5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidase S11 D-alanyl-D-alanine carboxypeptidase A N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0370059</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8161849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UDN5"/>
    <dbReference type="OrthoDB" id="10254188at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009002">
        <property type="term" value="F:serine-type D-Ala-D-Ala carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.710.10:FF:000091">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001967">
        <property type="entry name" value="Peptidase_S11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21581">
        <property type="entry name" value="D-ALANYL-D-ALANINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21581:SF6">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00768">
        <property type="entry name" value="Peptidase_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S11 D-alanyl-D-alanine carboxypeptidase A N-terminal" evidence="1">
        <location>
            <begin position="146"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8161849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="395" mass="45346" checksum="E454221236ABFE22" modified="2022-10-12" version="1">MKIDNITPRQYILRLNHHPPDYRLQIQNSPVQKLNKQIDLTNKSITESIKKKIQLRKISQISSYQHYDPINFPQSNTMSIDTKQFHIFTKKNSRANTCIQQLSYTSDYSINHQKQKKPQQPQTAIKRNKRVSPIHIPLCPSNPFGPPITAQSWCIINGKDGSMLAGYNEKLKSQIASITKIMTCWLSLKFISLISLDLDNTYFTVPEAAEIIGGTSAQLQNGDQLNIRDLLYGLMLPSGNDAAITLKYNFEQNIGINFIDEMNNCAKEFGLKSTQYMNPHGLYHKFNYSSALDIGILCYQTLQNEHFANIVKTKIYFSEIIDKFGNIKEILWENTNKLLDNGFQGVKTGQTKEAGPCVVEYYQDNNNSYIIVLLNCKSTDQRWDDTIKLLEWIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UE13</accession>
    <name>A0A8S1UE13_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0370102</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8161932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UE13"/>
    <dbReference type="OrthoDB" id="19174at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24124">
        <property type="entry name" value="ANKYRIN REPEAT FAMILY A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24124:SF14">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_25, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13857">
        <property type="entry name" value="Ank_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="785"/>
            <end position="817"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8161932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="849" mass="99302" checksum="256CBA4C8F913353" modified="2022-10-12" version="1">MKQHANNYFVKKIAKPISQVSTNSAVTRSNEQKPISYLTFTNSKHKRKNETAIKLPELQPSSIRAKTEQDQTAQNELKRSIVIISRKPSATLASQRIKTSPSIQIWEPEQISQQPVEEVQQITNKFYDVLCEELLLNKKQQKFKEKRKIFCKTILNEYDKFQEKSSRMVRKIIQLLKTHREGNEEKEMKSRTIIETKMAKSSLFFHAQTLYLPEQHQFNQKELLFQQQLSAIPKRHTKYSSQLTLTQQAESLKPIKTLIEEIKEKQQDPIKQASICLVQPKHPSNNKKKIKLIIECPEEQKSNKFEFFIPDYMEQDQIVSVQLLNDQIESKDTKKSNIYSMVSKNMFSKKMNQIQNVSETTMEEFNSDKATQFTRYYLQNYALKCINQKKIKDKKDSESQLNIMKQDSKQEQQIFLSKQLLDCSITKDLQATQFLEINSFIYRCIEPYDSQDSSMISDDSLIDDKSFIDSSIDQGIKLKFLISSKLINPSIIKDKFIKFMISTISDESKNILLHQEIMAFANQIEEVIHIDYDLPQQPHLEALNRLFLRINKYDRKQFLQSFFKKFENICIGLFEDCYKHQLNKKEKIFVKLCFQQQGNSLTDNTSIKYSNMLKSRHHRETTTETVPKQQTNLMILSLPPVTIKSRSESPSNFSEPRTQKKGIQNQQVLLKNMQRKESQRFLQIQTSSKFQIQQQASKQQVLVVEGKEQSKIQKVKLTQFQNNLKQLSMDPQSLLYRNKMIENTKNVVKSLEERIFLMVEEHKLLDLKEILKLETSIDVNYQNDEGNTMLISASKCGAVLIVEFLLKNGADASIQNYNGLTAMDMALKNYQFQTADIIYKYLYPDNKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UEY9</accession>
    <name>A0A8S1UEY9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trichohyalin-plectin-homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0370276</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8162252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UEY9"/>
    <dbReference type="OrthoDB" id="310405at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38019">
        <property type="entry name" value="KDA ANTIGEN P200, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38019:SF1">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="198"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="517"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="565"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="225"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8162252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="638" mass="79061" checksum="C59B37575CEA8AE7" modified="2022-10-12" version="1">MHITPVSLLNFDQTKVRDPIINSPRSIEACRRQGIRPEQLIMKSLYDLKRENKNLNLDEQAWKIFLQHEEDKRQARVSVLIAEREEIISQQKDEYENEKFERIDLEKERKRMEMIKNRRQKQMQKALDEEIKTQELQNQLRKKEMELKERQNEQRQELEQRKKDKKIEEQFRKQQIEYQEHLRKQKLYAEQDRLKYETTHKITPQQRIQQEKELKKQKNKRDKQLKKAQEEREEKERVHQQMLEQLYEAKLQKMEQREARRQILLEEEKELRQQLIQEKQEEANYRRDENKRRAQHQLNQVYLNFQQKQLNAEQKLIAKQKEIELLNKEQDMKRDIQKLKRQEIQKKNTAQQEKRKDDLMFKFKEIEDRFKINEMDRELTLHQRRQLQEEREHYRQQLLLKNDQELEKKCEKISMQREAKELQALKVLKERDDMIKEKHLKELLRKQEQEETQERMMRIRDYEREQLKKKVEEKSQRRDQLIKEKIASEKQKQEYKKAQIMEKEQLKKDLAELLKEQQQQEPKHINVQHKKRPESAPQKGKALQEQQQKNKPIVMAPYDIMTKEKQIQELKIKQNQELLNLLQEEENKELDRQKLLRQVDDPIERMRLEKILEFERALTQDRLAQLDNKHQKELEKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UGY2</accession>
    <name>A0A8S1UGY2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0400222</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8164100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UGY2"/>
    <dbReference type="OrthoDB" id="354678at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="153"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8164100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="482" mass="56472" checksum="44B33DB14C8879CA" modified="2022-10-12" version="1">MIKTKNIVQIEKIDNTDWPIFKCDQSRNNIFWDKKNDQKLDTNQFIIKDNLFRIGKNKSVPKQYTYLLMKSGILIYQDSKIKGKIQLNLKILIKKIELTNEEDHLQQKIVAIRIQKYQNCFIYIWNPENQACTINWYKKLAQFCVSQNFEGFYKIIEPIDQGAFSSVFLVSQLMPEEQRNKKFAAKIYSQSLQQKISAKQMRDFVTSECQILKIAKSEFIVELFEVIQLEDVIILILEYIQGGTLSNLIKQNEIHELMSSEICHQIILGLKEIHRCGYVHRDIKLDNILIYQFNPIQVKIIDFGFAEKINKNQLINKQGTPGYIAPELFDLAPYTENCEIFSLGILFYILLCGNFPFRSTNYEDEWKNISSSAQRMVQRMLEKDPIRRITFSELTILLELHISNLKSKGSHLRQNLNQSANSSNIRGITYEIIEKSPSKLVQAIKDNDDDDIGLDENSLNNNNIWNLQQSCKYYNKQKWITQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UIS9</accession>
    <name>A0A8S1UIS9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0400304</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8164264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UIS9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="17"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8164264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="205" mass="24479" checksum="8D3208FBE15DD01E" modified="2022-10-12" version="1">MNTVIEQNKLKNLATYINEEERKFQKHLEKTEKTNQKRIDLEEEIHKLQLEIIKQNHMKENAEKLQKDYPQLLQERQNELINLQKDLALATAAKKAAQQEFDIQIKHLQQQIAELRREKQDLKRENLGKQEKQTALRNDLQKFGKALQSREKPPVEYKIYSSSGGFGPIYQPQEPIYQPLELSINRPIDNIYQPQEQQYQQHIFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ULM0</accession>
    <name>A0A8S1ULM0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PWI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0430158</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8165850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ULM0"/>
    <dbReference type="OrthoDB" id="163257at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048024">
        <property type="term" value="P:regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002483">
        <property type="entry name" value="PWI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052225">
        <property type="entry name" value="Ser/Arg_repetitive_matrix"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23148">
        <property type="entry name" value="SERINE/ARGININE REGULATED NUCLEAR MATRIX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23148:SF0">
        <property type="entry name" value="SERINE_ARGININE REPETITIVE MATRIX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01480">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00311">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51025">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PWI" evidence="2">
        <location>
            <begin position="27"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="125"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8165850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="171" mass="20334" checksum="E882EBEF66FB7833" modified="2022-10-12" version="1">MAGNFFKGTSTDQDSRFGDKERKLIMNKQWPEVFNRKLNMKNIDLSVIKPWIEKKMIQYIGIEDEVVQRQIINYLEQQSEDIRGPDPKVLSIQIMGYFEKNTLPFMTELWNLLVDAEGQDSGIPNQLLDSKKLEYEEKKKELQRLLERQKLLYQAIEYAEKTRKKTKTEQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UQL1</accession>
    <name>A0A8S1UQL1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycine cleavage system P protein</fullName>
            <ecNumber evidence="5">1.4.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0470151</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="5">The glycine cleavage system catalyzes the degradation of glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[glycine-cleavage complex H protein] + glycine + H(+) = N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[glycine-cleavage complex H protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:24304"/>
            <dbReference type="Rhea" id="RHEA-COMP:10494"/>
            <dbReference type="Rhea" id="RHEA-COMP:10495"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="1.4.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 4 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the GcvP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.4.2" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDO010000047">
        <property type="protein sequence ID" value="CAD8167498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UQL1"/>
    <dbReference type="OrthoDB" id="6537869at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016594">
        <property type="term" value="F:glycine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004375">
        <property type="term" value="F:glycine dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00613">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000005">
        <property type="entry name" value="Glycine dehydrogenase (decarboxylating), mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000007">
        <property type="entry name" value="Glycine dehydrogenase (decarboxylating), mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000224">
        <property type="entry name" value="Probable glycine dehydrogenase (decarboxylating) subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003437">
        <property type="entry name" value="GcvP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049316">
        <property type="entry name" value="GDC-P_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049315">
        <property type="entry name" value="GDC-P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020581">
        <property type="entry name" value="GDC_P"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00461">
        <property type="entry name" value="gcvP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773:SF1">
        <property type="entry name" value="GLYCINE DEHYDROGENASE (DECARBOXYLATING), MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773">
        <property type="entry name" value="GLYCINE DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21478">
        <property type="entry name" value="GcvP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02347">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 4 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Glycine cleavage system P-protein N-terminal" evidence="6">
        <location>
            <begin position="40"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Glycine cleavage system P-protein N-terminal" evidence="6">
        <location>
            <begin position="477"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="domain" description="Glycine dehydrogenase C-terminal" evidence="7">
        <location>
            <begin position="792"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4">
        <location>
            <position position="726"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR603437-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF21478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8167498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="972" mass="109041" checksum="361BFCB54621CD35" modified="2022-10-12" version="1">MLRSIRLIQRVANFSRLANPHEVNPKVPDWFKSSDYIEPRFIGSEPQQVKEMLKSVEANSLDELVDKIIPKEIRSEAAFQSPDNFPDAITESAMVQHLQSLANKNKIYKNYIGQGFYGTHTPYVILRNVLEDPGWYTSYTPYQAEISQGRLEALLNYQTVITELTGMDVSNASLLDEATAAGEAMFLANSWFEKKKFFVDNHVFPQSIEHIKTKAYYLGIDIIVGDAKTYDFKDADQYCGVLVQSPDNMGEVHDWSDLFKHTLKDAKLLKVIGTDLLSLTINKTPKDQGANVAYGNSQRFGVPMGFGGPHAAFFAVEDEFKRKMPGRIIGISKDANGRSAYRMSLQTREQHIRREKATSNICTAQALLANMAGLYATYHGPQGLQKIANRVNCLARSFAKLAKSLGIVVKEGRIFDTVVLHNTENLQEYLHYNAQINVRKIGQDTIFSFDETHTLQDVEDLFNHLQHFTKKKADFMSVIQKVIPYKSERAPYMQQKVFNSLHSETEMLRYINYLRQKDISLAKSMISLGSCTMKLNPTSFMLPVSFQGFSQLHPFSPLSCTQGYQELTDNVEKWLCDITQMEAVSLMPNSGAQGEYTGLLCIRKYHIMNGQKDRNICLIPISAHGTNPASAVLAGLNVVAVNVIDGYVDLNDLNKKIKENEKSLACMMITYPSTYGVYEDQTKKIIQLIHDHGGLVYMDGANMNAQVGYTSPGYLGADVCHLNLHKTFSIPHGGGGPGLGPIAVNKKLAPYLPGREHSLGSVASSLYSSASIIPIPYSYFGQLGRQGAKKCTAMAMLNANYLMKNLKNDYKVLFTGQNGMCAHEFIIDIRPIKQESGITEEDIAKRLMDYGFHAPTMSFPVPGTLMIEPTESESKSELDRFIEAMKNIKLEIEKVKNGQYDKNDNPLKNAPHTQDQVINSGWNHKYSREEAAFPLPFVMQRGKVWPTVSRINNAFGDRNLICQCPSVSDYQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UT70</accession>
    <name>A0A8S1UT70_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0430060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8165656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UT70"/>
    <dbReference type="OrthoDB" id="299332at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8165656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="225" mass="26077" checksum="C089195222AD63AA" modified="2022-10-12" version="1">MYFSNKSSSFSNAETNTELNMLFDSMIHVDQMSKMNNTKIRLYCQLTQYEGKLPNFNELLFDPSSLGRYKNFIGASSKIANYIKLLDPKIRKKFIVEEKYDDYLKQSKHTKIECERIKALIDQHNAIYTLNLKGIAGIDYTHKSNLKKSPQKLASICTARQSESTKNDILKDVDSSRIHATQRGRKVKVKPLKQKQQSELIFNDSPRSVPLQPIKRSQLYQLFDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UTW7</accession>
    <name>A0A8S1UTW7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0470018</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000047">
        <property type="protein sequence ID" value="CAD8167262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UTW7"/>
    <dbReference type="OrthoDB" id="298217at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8167262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="232" mass="27934" checksum="812D7EE28400DDB3" modified="2022-10-12" version="1">MNQTTPKSIGINYAKLRKQKGKEETLPKSMSTNLKFDIYQNHQHGILKHRSTSQHNINEYTQQQQQMQLFEQYFNHSKISFTFIEFKLINPNIFKNSQRKWEEERRKILYELNQQKEDNCILQTELHDLKRQKNTLSKQCDNLQVIIVQERLGKQQRHQQFYQHKFQELTKNFHMPTQETISSLTDERFESNETSLDSSLSNEDDDPSIYSNQLFEILQTGKHRYQFSKKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UV47</accession>
    <name>A0A8S1UV47_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0480199</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000048">
        <property type="protein sequence ID" value="CAD8168123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UV47"/>
    <dbReference type="OrthoDB" id="160405at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8168123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="130" mass="15141" checksum="5561C29EC9CEB565" modified="2022-10-12" version="1">MNNKNDQVFKEPPPYYFFGIPLHNKRVAQQFCFIFILVLIIPFSMFFGLRESLGEVSAGISAVVSIQFVIAYYVIMIIRDPENFPNADKARKQIEQRKQQTKQNQVETEPIKQSGQKKDNQSQKNKLKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UVV1</accession>
    <name>A0A8S1UVV1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0490179</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UVV1"/>
    <dbReference type="OrthoDB" id="295358at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="371"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="441"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="136"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="204"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="381"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="449"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8168564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="516" mass="60906" checksum="3D3594409517DD3C" modified="2022-10-12" version="1">MDSQQDVECDQCQTVPETYVKLECDHKFCLICLAYNYLQIQQEKQNLEELERVAVCFKCNHLTKLDQDTVEALHMVIKEIIVPLIEQNQINSKSNNDSEVVEVIDDLRDLGVQNDGSQHQSNQKNSMANERLQLYLERLEKSFKSKFEAIKDIQDQKHQFQSKCKQTKDEINSQYQQFILSLEQKKNQTFNDINSLEMIQLLEIQKQEKDLEQQIQQMAQFQEEINSLMYQNLNKSQEDISKLLIDIEKSLNTPSSSLQIKSSILATQRQLLKLSTNAQSQLSATVPNLLQDFPQSIRQKDLFMKNQNSDEKPLDSKLNVHFERQDRPNKFSPIKSEKLNELWPKNDKSHLSLEKNTIRESKDLNEFKDYREKEKDKKNQKVLTQESLNANPKQKFIYEDSDDKSRNNNNSKKLFDSQISQKNNLVSKWKDQLFDVFDKKTMKQSESPKVPEKHNYATRDVPYHREPSTHYPRENKNSQEKLSTKQQLINRACTIHDVLNMQYQNFMTQKENLIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UY39</accession>
    <name>A0A8S1UY39_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PARP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0510150</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000051">
        <property type="protein sequence ID" value="CAD8169595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UY39"/>
    <dbReference type="OrthoDB" id="283889at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001510">
        <property type="entry name" value="Znf_PARP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00645">
        <property type="entry name" value="zf-PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01336">
        <property type="entry name" value="zf-PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50064">
        <property type="entry name" value="ZF_PARP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PARP-type" evidence="3">
        <location>
            <begin position="5"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8169595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="143" mass="17233" checksum="2DB43E9321A1114E" modified="2022-10-12" version="1">MKQYIEVGYALSNRVKCQNCLQNIIKDDIRIGHVLTRPPGLGFDKKIWYHLNCLTSIKGDRNQDLDIVNIHGLKEEDQKKVRQKVEQIKKSSYQKKDQKEVKYLSKQEHFQNYVKIQRDLHFNQKLRQQAMFFEKIDQPEEQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UY66</accession>
    <name>A0A8S1UY66_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0500013</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8168832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UY66"/>
    <dbReference type="OrthoDB" id="298112at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8168832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="602" mass="70519" checksum="A4EEC8DC9171BEBC" modified="2022-10-12" version="1">MQELKSPKFLNNLVSNTQKKQNPIPPSTDINILQQILDQVQPVEILHMNYNDGQEQTNQKRNNKTLKINEDQAEQQLIDFIQLSIQDFEANQYSQCVYHLKQAEQILTAISYSNPLIHYFLIIRINLGIVFYAIGWLEQAFTCFEDAILALKKTPRTDQEGYLLSIIRLHCYLQCCIILSESDQHSESLAFAKMAIRKSSKILIDLQKYMQSQIHQQVLEEILRKSIPILKPKHQTIVQGYQIQTQTTQESYDLVYSECPQTYYQRANILYFIQMNPINLNNFIHEMDFPTNQQYLHMICLFVISLYCTSTESKFVQKVESFCFSEAENYLSRALEIAYLYLPKDLPLINQLLNVHIKFYDISKQAIDEDAKIKMDQGNFEIVLLKPIIESQKCGFVIPIIRKSKYIGMKSRTRSMKEQLFSNTKQANFNIEPEQQIYEVQNKTHKQKLRSISTTNKYKPNSSKWVNHQLAEVYLQQQYPFKSIQIKKYSQKYNNKTVELGSAKNSTNYKSKVNRSENQNYKKQLNMQLMITNINQNTNSGQTIQIANVIQLLLFNKKKNNQSINFDQIITKSPVNLFLQKQKDDKKLSTIQQKFKSVLKQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UYD0</accession>
    <name>A0A8S1UYD0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0500048</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8168902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UYD0"/>
    <dbReference type="OrthoDB" id="307760at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF369">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="283"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="731"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="793"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="820"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="852"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="886"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="10">
        <location>
            <begin position="37"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="8">
        <location>
            <begin position="149"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase C-terminal" evidence="9">
        <location>
            <begin position="748"/>
            <end position="904"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8168902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="922" mass="104952" checksum="C289436BCD3E1EA3" modified="2022-10-12" version="1">MYKVSKEQLREILASGLTQNPQDSMNEFNTTKRFEDFLKCNITQGLSDNEIKQRQEVFGINQKENLDSISFLNAFCIILLQLKSIFFLTLSLIAFILKYLSTQGIYTNEWIESLFIFGLIFLNLIISAIHLKIDDNNKKKLLIQDEQLKQVKVLRNGQEQNIIARDLVVGDIVILQDKDKIYADGLIVEQNNLEIDECYLTGEQDLIHKITLEEANQQKQPILPQKHLIFSESRVINGNGKLLVLGVGIETQASRIQICIKQENNNTPIQSTIENISSKMETIGFVGAIIVIFMLLARYMIQYHDSKVNGYSTFSNILNLIIVLISGNASKALITHYQLQLAQTVKLMLYSQNLVRNLNQLENLPFMDQLLIDKTGTLTQNRMALNCFMNDNTEELTKSRFNQFPQEFQQVFIDSCFINNQYIPETDQGSYTDKELYQISQEMRINLEERKKQVTHIIPFNSARKRLTSIINNNRVVVKGAAEIIFQQSTKFYSLKNGIVAIDDFLRDDLEQHLIQLSQEGRTIAIAYTDVNLQNENVNELIQNNQFQFDRQNLTLLGFFQFSDPLRMETKMTVEGLKQAGVKVVMITGDNSVTAKSIAIKAGIMSLDSIITEGAEFTQRIQNKAENFSKYFEDIFVLSRARPEDKYNFVKELQELGHVVGVCGDGTNDAPAISKSDVGFSMGISGTEIARESSGIILLDDNIHSIYKGLILARNLLDSIKRLAQYQITSHFSLIIILIASSAIGDAVISPLQFIWINLITDIFATFALSYCRPSSQLYHQNPPNKNGFLLDFIVYLHIIILAIYIITVCIIFINQSLMVFNLFVMTTLFNLINSRMVYLELNVFSGFFGSWMIYTSILIIGLLQFLIVEYGGIIMQTFDGLNLKQWLICIAVGIGSVIWRTIAILIIRPIIKSEKQKLKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UYT5</accession>
    <name>A0A8S1UYT5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0510028</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000051">
        <property type="protein sequence ID" value="CAD8169397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UYT5"/>
    <dbReference type="OrthoDB" id="20534at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045234">
        <property type="entry name" value="Unkempt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057444">
        <property type="entry name" value="Znf-CCCH_AtC3H23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14493:SF50">
        <property type="entry name" value="RING FINGER PROTEIN UNKEMPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14493">
        <property type="entry name" value="UNKEMPT FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25512">
        <property type="entry name" value="zf-CCCH_AtC3H23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="81"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="114"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="194"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="236"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="327"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8169397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="481" mass="56995" checksum="8A224B9E5F16CDCB" modified="2022-10-12" version="1">MLGSRRVRSHDSSLTNLNGYNQNCQVRVHINHHQFKTLKCESQHQIDQKKFCPFFHDESDRRRNLKEHTYQQIGITIYFLIFRCQLCPQANKCPRGDQCQWAHNKVEQVYHPNRYKTKYCTHLKDCEYGIYCSFAHSEQELIIPVKLDGMVQDKNFWIYQYKTVWCPHTINHDRASCVYAHNAQDFRRDPKILQPKECPHWNKTNQILNYDKGGCPDQEACKYCHGWKEYEYHPLIYKTKPCTQQNCTKKQGECAFFHSDQEKRLRKQIGENSWVIEEPNIHVEAKRQPYKNTSNYLGPIIPNYIPQDYLNREKMEIGQPFCQQSITNTKTSDSQSRRGSDFSDGSKTQKKKHNIQQQFQSQKKPRTAPVTPDQKQLIGSNQTMKVQNNVQNNSYLTYSKKLYEEILKLHEGEYIYKILQGFKIQEKMLMQMSDEQIQELNLTQYQITQLIQALNAIKQEQRYDETCGDEILSLISNQGKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UZ10</accession>
    <name>A0A8S1UZ10_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0530019</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UZ10"/>
    <dbReference type="OrthoDB" id="306198at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00052">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216:SF174">
        <property type="entry name" value="GH06923P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00027">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50031">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EH" evidence="2">
        <location>
            <begin position="10"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="EH" evidence="2">
        <location>
            <begin position="152"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="185"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="276"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8170358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="440" mass="51444" checksum="EB5FFF9604BC6AFA" modified="2022-10-12" version="1">MNNLQLTPEEDQYFRQLWSTLDPQGIGSTNAKDTIQFFKRSQLPSDKLKAIWLMAVSNNENKLYLQEFLIIMKLIAYCQNGFELNPSLLMENQYVRLPVIDGLLLPQQPSPSLGAYQQMPMAQMYQTAPVLNQQQYKPPSDITNQFDISNELYDKYSLHYQQQDRKNQGYIEGESAKAIFYKSELSNEILKELWALVDTNQKGFLFKNEYIVAIHLIALCRKNIPLPQYLPDSLQRLIAQSQPQQSIIIKDIKVQYDTPQISQSNNCRVQSGQQVQNQQNNELQTLTMQQQQLQNQLNQLVMQFQNNSNNNAQKQLIQQKENDIEFLIQQMNKLQLIFNSLKQQEQELQTEIQKLQTKPQIQKPTTQQHQQINYSQSMYQAQQQIPQYNEILNIPHNQNISSTNYPSLSIDNLNHQGIYQLKDGYENYQNQNTGNQDIPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V022</accession>
    <name>A0A8S1V022_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0520004</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000052">
        <property type="protein sequence ID" value="CAD8169853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V022"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8169853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="90" mass="10271" checksum="F8A54940D5163580" modified="2022-10-12" version="1">MNLKTLVFPFNHAMQINNQNPCRGENKNNVAYILVNVWISKIAAQSLKIIVKYHHINVSDGSKLIMMLECSDQKAKNKFANKNQNGVEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V3Q5</accession>
    <name>A0A8S1V3Q5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0550092</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V3Q5"/>
    <dbReference type="OrthoDB" id="296577at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8171571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="214" mass="24179" checksum="C641427ADB5CE2C9" modified="2022-10-12" version="1">MLNSSIYSNESNSAIKTKGNQAHLLSTAISKLGDSSFKSRNQESKNISPVRLRLFRDSPKTYKGLEGCVVGQYHPLPPTHVVYSNDKTPSPYRSARTFKDSPERAKVKLNQFKDWNSKLDIQLQIQNNQIAKLNNQLIQQSYQPYQHKSSKSNLFSTSSPSNFQKFGKQSDISSLSSRLNQVPISQIVNYQLGHQSELNALQSSLSRIMKTSRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V8W7</accession>
    <name>A0A8S1V8W7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0580205</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000058">
        <property type="protein sequence ID" value="CAD8173081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V8W7"/>
    <dbReference type="OrthoDB" id="186625at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048306">
        <property type="term" value="F:calcium-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051426">
        <property type="entry name" value="Peflin/Sorcin_CaBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46212:SF3">
        <property type="entry name" value="GH27120P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46212">
        <property type="entry name" value="PEFLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="722"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="789"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="826"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="402"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="72"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="143"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="225"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="419"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8173081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="880" mass="104227" checksum="3200F142EED0AC65" modified="2022-10-12" version="1">MRPYTSTPQIQHREPAHSAIEFRQNVQLFKPKYYVTKAHQDSNEQFLSSKTRDPPNSREFKKTIPITMVQDKERLYEELNKTKQMCNNLKLENHQLKAQLKSQETAMLKCSDPEKEEYQASVINAIPYQYKKQINEVPQSTIILQLKKQNKDLREELDDFKEEMIRVKRSAKLTKIQELEAELKIYSEESLRLKQLLHQQILKTMNPTKTEQSIEDSILALQEQLQKCVSQRNKQYQELKKAQEEIEKLNFLNQELEQNYQKSIKEKTQLKKQLTSLEEQFNKAQLNDPLFKRGQDVTQMKLELEKKVVENNQLKNDNMIKDRKVHELERQLKDVTSTFQDKLNTVTKEKESFKEKYEKQKQELIELQDKYQQLFFNSNRNLQSQQMQSGRREVAFTVQSQEDIQNQRRKSQEFTPEVQKQNDTQQQNQQSIQQEKQSYRRKVQRKLSSIRQDDIEELLTQLRYKLRAQNIRNAELELYLFRQKDTNETSLGELIQILQNKPFNLNQNESVQISRYLIEPYGEKEITYNINLTKDNESIMQTLIRAIGKYNILVGEDKLQMHEQIRQKLNEKKDDLIAFLKPKKQQGNSQQSISAQLPGSLVNRQQFKTALLQTSLDEEQTDVLLQTLYEQTQDFNFIDLDMVFQTWPSSGPIKITRSQIEQLKEDGELPDIVRECIDSLLEYANKQGLSILDLRRQIDEDNSGHVERTEMKQFLVKCDIQLNNEQYDLILDHFDLEGDGRISTNDIGIVLNKAYNQKMLAMKEQRTTKKTSKQILNLVASHFLQFMQINKLDLFQIFMTIDQDGSGSVSKDEFKQMLRTRIVIPLDQEEYADFFKLIDNSNDGQIHFNEFQQHMIPEIEKITQRPYHELLSEHITKKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VFW8</accession>
    <name>A0A8S1VFW8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0610274</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VFW8"/>
    <dbReference type="OrthoDB" id="245563at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="136"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8174769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="186" mass="22180" checksum="1E7F193ECE54DC11" modified="2022-10-12" version="1">MIQGLISIMLQSWESNCKVKNQAVKALKIDPKHFNSLYKKQSYFDKILQLKVQVSLTNITKPLYGQIKLCRLIQSIAIPYQLKVLIFQKFIDISLQFNILVLIQKMLKIMKKPQLQLINPFKQIPIIQFHYGKNFRKFSIYLGKLLQEQNYYQQALMYYGKALKLKKNHQRPKDRKNRYLDALKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VN45</accession>
    <name>A0A8S1VN45_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0700141</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000070">
        <property type="protein sequence ID" value="CAD8178854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VN45"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00157">
        <property type="entry name" value="Rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000983">
        <property type="entry name" value="Rho family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="181"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="181"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="198"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8178854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="208" mass="23286" checksum="01AA4EAB2177104A" modified="2022-10-12" version="1">MQGSQESVQIKLVVVGDGSVGKTCILLSYTTDKFPTEYVPTVFDNYTTQLTVDNQMVNLSLWDTAGQETYNRLRTLSYGSADIFLIVFSVADSSSFDNVLTKWYPELNQDELQKVPKIIVGNKIDMRDESNSKHIKKASAEQVLANQNLQYYECSALTQEGLKVVFDEAVKKALQAKSMNKPVTKTGSQNKEINNGNQKKDEPCCSIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VU90</accession>
    <name>A0A8S1VU90_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0760044</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000076">
        <property type="protein sequence ID" value="CAD8181274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VU90"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8181274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="84" mass="10084" checksum="E1F9136F0A66B7F8" modified="2022-10-12" version="1">MITDQVGLFIHKIPILKDNRFFSLLRMEFIEADQIYLMSITPNIDKDTQIKDYLTSLIVSTLQLINFQQIQRLSLYQIENHYQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VXP8</accession>
    <name>A0A8S1VXP8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0750212</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000075">
        <property type="protein sequence ID" value="CAD8181123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VXP8"/>
    <dbReference type="OrthoDB" id="306181at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="265"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="356"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="176"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8181123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="535" mass="63525" checksum="7F0D5A6D5479F22B" modified="2022-10-12" version="1">MHKFKMIENEKDLYCRMKHRAPVQMVILDSKLKLNQKLLCQQCIEYFESDFTKVIGYKKVILMIEENRKKSFDIIDNLITKNIKKIELFQNHIIKLKSKVIQWLDELIGDSKDWILNTQSTISNYVEYSFFKEIDLIIMNQESTQLDLNIFNNQIKTLNHSWYTKLNYKLEQFKSFQEYQNLQDILKNQSQNLLQQQQQQQQQQQQSQQQLSQNILQLIDDSNYQIEPCYAIVFDPKGNIVVSTKGNKIEIWNFNNGRIKLIQSLQEHIGDVNCLIYSQIKTYFISGSDDSQIRLWKQLNNNKWQSSQAHHHSAGVILCMILTQKEDQLIISSEKETIEVWQVDFVNNELRFLQILQEHKSSVTSLSLNEPEKKLVSCGHDGKIIIWQKEPNNLWKFWQVVKQSIQYVGLHVKFLKEDQFIFIPSESNIVCLYQLKNNQYQESIEKKVQFKKNTQGVVNQSFFPIIYNKEKDLIFLRHIFHICILKVQQGGYLQIVQEFDCTNGYIYGTISNDGNYLIFWGQEKGKYEIYQIQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W1J4</accession>
    <name>A0A8S1W1J4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0800033</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W1J4"/>
    <dbReference type="OrthoDB" id="10252009at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000118">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF519">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE MPK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="6">
        <location>
            <begin position="14"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="7">
        <location>
            <begin position="78"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="236"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8183011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="293" mass="33478" checksum="96AAA738A0F56AE3" modified="2022-10-12" version="1">MFRMFDPMYQSMNQILEENGNILWLGDCTAAYDRSLLDARGIKTVLTVAAGLNVSYPEGGIVHKVYHILDIESANIARLFGDTCNQIGEGLKRGGVLVHCAAGVSRSASAVIAYIMKTKGLSFQETFNYVRKRRSVVFPNYGFQRQLRNYEKDLKTIKVKEPQGIEMPMKQTQKKPQEIAMDKHEQFSIQYLEQKAKLLLKPVSGNPTFNSGYSTPQKQFQKQRMLYQSAKVGSQEPKQSSITQNSTRANSQMKRNIFEPSFNQTYQGPQIVTYQKNKLIEKMPDIKKKTFYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W3L5</accession>
    <name>A0A8S1W3L5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CPL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0800159</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W3L5"/>
    <dbReference type="OrthoDB" id="310624at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012959">
        <property type="entry name" value="CPL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040059">
        <property type="entry name" value="PUM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13389">
        <property type="entry name" value="PUMILIO HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13389:SF0">
        <property type="entry name" value="PUMILIO HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08144">
        <property type="entry name" value="CPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="repeat" description="Pumilio" evidence="3">
        <location>
            <begin position="78"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="CPL" evidence="4">
        <location>
            <begin position="349"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8183257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="537" mass="63528" checksum="C387B02E38B247E7" modified="2022-10-12" version="1">MGKKRIVEENQNEEPEEVQEVIAKADNKKQKKIAKNQNKISKDVDFISFLKEDIRFLIQHRNKENEEKQQKAEEVYNKIEGILYQIAQTKIGGRTIQLVIKCADQNIRQQIFSKLLNDKQFGELLQSKYGHYIGITMVRNMLPEFRNQFFEILLKNANHYVAQGDASIVLDRFLTREATTQQINKVKQLFLNHSEKIDNLAMKIIEKGIHNHLLSLQILKVALPNLVPEERQKVIEYFQQLENLDYLRHKDGISIFCAILNVTDKKERKNFLKNIQTYMQQSQEHLHRNSQFYLALQKVIFTYDDTKQVNKSILQDILPEWLNSIHVFKIIQSIYQPKLRDDIKFDTIGLQNTVSLKDDEIRIQELQDYCFESILNSLQSSETNILLESNINQFICYFIQYIIKYQKVQALDFIKEQIKQLLYFKIDTIFSREVEQNGNYWILTQPEAQRIAKCLIQQQQIAQGEFNEIINNYCQKIKSLLIQNLKVTLISKAIYLLLALIENTNLKEQVLQDVKKNKKWIDKLEKTQSLQILYKYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WA83</accession>
    <name>A0A8S1WA83_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0840011</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8184799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WA83"/>
    <dbReference type="OrthoDB" id="593360at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17364">
        <property type="entry name" value="MFS_PhT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="176"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="307"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="354"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="388"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="475"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="28"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8184799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="528" mass="58623" checksum="3B855131D869E72E" modified="2022-10-12" version="1">MQKDRLLEKQGALDALNNAKFGWFHIKACLVSGIGFFTDAYDLFIIQLAVIMIGMVYYHEEIRDGDTIIKKADKLYPVSETLLKQSALIGTLIGQLLFGYLADILGRKKMYGTELLIIVFTTITSAFASNTASPNGLTVVGMLIIWRFFLGIGIGGDYPLSAIITSEFANTKNRGAMIAAVFAMQGFGILTGSIVSLIVLLSFSSKLNNGDPLNYLQIDNIWRIIIGFGAIPGLVAIYFRMTIPETPRFTMEIKGDVEKGARNIEQVLKQDDTVYVDSNQDEKKQKEQKPTFRELKYYLTKWDHGKVLLGTAMAWFALDVGFYGINLNQSTILKQIGFGGGSNLSQYEILKQAIYGNLITSALGTVPGYWLTVLFVDKWGRKTIQIMGFVALTILFLLMGLCKDILGQFLFITLYTLANLFNNFGPNATTFIIPGEVFPTRYRSTCHGISAAAGKLGAIISQVWFLGLGAENFKAIMLTFAFFMLIGLGFTFLIPETKGKSLEEICQGQEQSIEMCIYLSNYSQIIPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WAA4</accession>
    <name>A0A8S1WAA4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NmrA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0880171</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8187148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WAA4"/>
    <dbReference type="OrthoDB" id="284301at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8187148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="313" mass="35416" checksum="49853B94781E6B16" modified="2022-10-12" version="1">MSLLLLNASNRIAQGFLKVAAESGKYEKIICADIFPTYFTVQRLLKFKQSLSANIELFKIGDRQDLHDVIKQANNVLYVSHDYYQTTASKKNLLVATLDLVKNRNYKTVAYVAPVEHDHQEEIDEWKHLEVEGRRFIPQLVGIRSDITFGPQSTFTNKLAQRIYNGESIYFQSTGQSCAPIFTGDLEQIVAQVLAGSHAGKLLLAKGHKHIDFKSIIHLIEDSLGTTYKANLNQSFIEKVIHPTNNCIIGQQLYCPSYINLTKLIANYKALENTGYDQVVGDKLVDIEEYHKNNKIVIDQSLKADYQLSYLVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WCJ2</accession>
    <name>A0A8S1WCJ2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0870249</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000087">
        <property type="protein sequence ID" value="CAD8186783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WCJ2"/>
    <dbReference type="OrthoDB" id="298154at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8186783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="230" mass="27487" checksum="45D80583712C160E" modified="2022-10-12" version="1">MKYGSPKVSSNTIDVLDETLSPRKSIRPYVREKRSISSKVKDFTYDNQLFDKVDNKIRAYLQKNQVNYEKTLIKLYKLDSLKSDKAKRLLGELNQTKRKQQFSAVRQTKSMQNSHPSLQRERNNSLPSSKQMQMDLKQIQQELKKINPDIEHKVLMNISVFKFNQSNCARLEQDEWERVALQYPYKIRPKMTNFEQELDRLNSYYSRNENCLNQLMHIQKDLLQYKLLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WCK1</accession>
    <name>A0A8S1WCK1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0870254</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000087">
        <property type="protein sequence ID" value="CAD8186793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WCK1"/>
    <dbReference type="OrthoDB" id="312831at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001303">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="30"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8186793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="391" mass="45842" checksum="135BA05AFAA0A1A5" modified="2022-10-12" version="1">MQTTLPIIRLQSEYPFKKVISPEIELPAEFILVEKAGQGSFGEIYKIYNTITKTLHACKVEFKDTLQQNQQSLLVKEYNTLVQLKGIQYIPEPYQLVQQQEYKLIMMPYYGSGNLEELRKKQLHEKFSETCIIRLAYCIISAIESVQERKFIHRDIKPENFLVGSCGDHHNVYLIDFGLAKPYMDAQGMHIPKAENKGMVGTARYTSINSHLGSEQSRRDDLEAMCYVLMYLYNGQLPWQNLSCQTRQEKFLKILEAKQKLAIGQLEINIPTVLKQIYDHSKGLRFDEQPNYTKMKELCKEGFNKSRPYCFDWCTQDCKLKPYNDMNDGFKEYANEFWTSKQQQKGQITDQLWRSINKISTVQQMKEEDEQSLNEFPNIQTIKSFYKNKKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WCP9</accession>
    <name>A0A8S1WCP9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0860016</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000086">
        <property type="protein sequence ID" value="CAD8185829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WCP9"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="20"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="58"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="314"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="686"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8185829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="829" mass="96898" checksum="0A807BD60E6F3B30" modified="2022-10-12" version="1">MNSTSVKNQNETMFFLSTINLIFGIIKLEMFTIISNSILFGLMFCYLWYKYKSHRKHLIFEQVIVIYYCCYQLIQRGVPGIWFNIFSIQLDQHLVLFVTLTIVIAFTCAQVQPMVEISTLQTILEQLILIIIFLYNKYNFTSLRQQTQNRIIKQIMKNVRYYAFDKYQNEIDGEKLLQSEQILNSNATLFSYRSENQKLQQQIRKDLKIKCYTMQQIDIAQESQILSSKPSVFETLGGFLEFYQKDKHQMKNKVILALEYDELTGQKQTYRLYIEQFKQFQRIYTLVALVNLDDKIPSKQNKNIDKFKMSLSKIFTHKLKTPLNTTLGFLQALVQENNTIDEQVKNSFLKPAFINSKIQYYQVQDILDYVNSVKLVTYNVSKVNLYKTLQLIYDMIELQCRAKKIQVIFTINSKPFDVNDRPIYLNTDQLRLERILFNLLNKSYRHTQIMGKIQLNVDVLSENNQVQFKINDNVQGFKQEQFEQINNFARLQNKCIHGFRNSIVKRSTFKFSLTLQITNKLIYFLSDFQCSLQINHDLEEGASYQFFISLNCQQVNFQHSFEISQKNSSFQKRSSGSLKQNLPNHISLFTKSQNQISDKVLEEYEIEVEPLANKPLQLSTQVVQNYPQNALFQSHPIQQSVFPFQKSVFQKEINDKSLTISVAFDHSFNVKRAADSIDIRQDMNQTIMLVDDEPFNHDTLKLMLKSLGFKNFISAYHGQQCIELVKKHHKSIKVIFMDLDMPIMGGVKATQILIQQMQDGEIDHIPIVACTAHDDKDTQQECFTAGMICIIAKPVFIRSLQETFLRINEIKVKSQNLVFGSKGITSSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WDZ1</accession>
    <name>A0A8S1WDZ1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0880033</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8186882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WDZ1"/>
    <dbReference type="OrthoDB" id="366230at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005858">
        <property type="term" value="C:axonemal dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:003559">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:003672">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF12">
        <property type="entry name" value="DYNEIN AXONEMAL INTERMEDIATE CHAIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0969">Cilium</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="569"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="109"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="316"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="66"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="116"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="131"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="148"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="316"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="328"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="360"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8186882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="891" mass="102205" checksum="3473E7E553BD8E05" modified="2022-10-12" version="1">MQSISSEVLKAGVTKSNVRSNVTAKRPNQLGGGRTQLGPPQTGKDTQQSSQDNVIVKYKDSVVTPKELITKRFLQLAEQLNREEEQKLKQLQKPADTKVSASVMDSNMKSFHQSADKSSNSDLKVSSSNNPERSSFMESKQSDTYSDSDSKRDQSEQQEKKPKQKKGLKEEDLEENIPINLSETPTQCLMFIPSSFVNSEHGKKTGQIQRTQKYEQFIVEKIGSDNFTKRFAQTFNYIQKHKPQQCTKIEKNDTGAFTAVWDLYDASITDQLNEFEILQEEIKQSIENEYKQLNKNPYFLLPTELEAIKIHAERPVLGKETSEKSQSKTRSGIATNTKQQPINNLSGGLTGQGGDERIQDSSSSHTGTKGVTNMGSQQANIQQQVTKSHGKTSNPLPEQQQKQYEQKQDEQDIKEEEYMKQYVQRTNSQISEQEQIIYQSQQVQTSLKYVERILNQNLYHKQYIQYRNYPEVKFEKMTLNEDPKQRGGAFKRNFQNKLEDEEVVEKKQQDPITQLFSYKCQLSEQCKVTSADWNPVNKDLLAVSYSNRETSDGHIMFWTLKNPTFPERIITYPSKINCCKFSESQPNLIAAGTVDGIVAVWDIRRKSNKPITENKEMAGKHSDSVWEVQWVGKGAKGTDKGESLVSISSDSRIVEWSMKKGLEYTNLMNLKRVVQSSQKENLQEGVNFRMSAGFSFDFLQRESAMYLAATEDGTVHRCSKSYTEQYLDNYFGHSGPVYKVRCNPFFGDIFLTCSADWSCKLWNWTEELPKANFQQQNLQDEVLDFEWSPHTSVLFASVCKDGRLELWDLTKNNMLDPYFTIMPQDQQIWPAKTMVRFAHNSPVLITGDARGDINAYRLYGYEDNDPLQEEEKLRKLLYPTGYSKGQKEQKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WN81</accession>
    <name>A0A8S1WN81_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0950122</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000095">
        <property type="protein sequence ID" value="CAD8190081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WN81"/>
    <dbReference type="OrthoDB" id="296921at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31513">
        <property type="entry name" value="EPHRIN TYPE-B RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31513:SF2">
        <property type="entry name" value="MRAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035773076" description="Transmembrane protein" evidence="3">
        <location>
            <begin position="16"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="578"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1020"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1059"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1094"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1127"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="957"/>
            <end position="984"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8190081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1158" mass="133977" checksum="463C63D0BA840957" modified="2022-10-12" version="1">MLKFLWILIVQQVNASQCECNQSYTDVCLINNNCSLNIFHQNNTINSQEIVQIENCNVQICLVKLVNKVILNFSQLIINNSKISQDDECDFIQHQQIVFQMDQLNIYNSSINFLGQMQIQFVTYNMSLYNTKILSKTMSICSQYLFISNSQLKSLMQNKTQCSCLNNLNCSNQAYFKSLFSFVGYITQNYTNLSAQFIESQWLSQFSCNFFTLKNLTYYTMLISVQQSLSLTNQSKIQGFYIGLYSYNLSIDNSSQILTDGMGYDTNEGFGCGYTDKIIGLRQQCGGTGGSHGGMGGFSTSPKSNYNQLCLQLQSKQIYDYSFNPILPGSGGGGVTYGDFFEKINIQGQGGGVIHLEVLNLQNDGIISSNGTSYIEEPFFGSGSGGSIQIRIIYFSGQGYLTADGGGSSYAEDFNQYEFARQKFYQTYQVYGGFGGGGRIRLFFFNITEMIHSPYYNICTNKIQTRPGGLTNQQNNVLIMSNQSLIKFQGSITPTGCPQGTFGNYCQQCQFGKYKLLYGQGPCQPCFNTINNNKTYYNQSGESTPFCTVECSDNKQPKEYQCLTGLAEFSQKLGGQNVIFALFVIIMLLLINIAIVWASRDKNQKKSRQFYGSYDDSTLQQMTSSLSELNNKTYLISQDIHFHVRRIYLAGNNTYQNPWQIYQETLVDSDLNQGDNQRLYRLFETFNQKALFTTFEKYMLIFFKFYYYPLYIWILKLIQVKKFQILSKLFIQQEYLFNELDPDRDKIKLKFSCSKDKTLAFIDRLNLALKIVEQSNTIELPIYLVLSGFGTFPYPFQINIHDALIKRLWQQFSFNTNEDSINLFEKFVAEFNYYAVKIDFRQSQSSFTKRFIDLLNYTNNYNYEIFRLHHAIQVDICIHIIANEVSAQSQVYLLGNANQRELEFLLKQIHIMKNLPEEFTIKLSIIFNNYNKDRIVNLETFNNLVYNINEQLQIIVNQDKDDLRNQIQQEKKKMQSKINRFKIDGMKQYKLKGFQVVKRFFTWLLSQITRYRDSRINQQFLIAGICIVLAIQWCFVIAYPVLLFIAHDMENDQIFLAEFITQSIIFPAAQIISLIILSAWLLKSRRNYGKMFLLFNLCALVSSLIEFIFGCYDLFNFDSSAEFNLFKLFPFFFILLSSHLVCLQMNFNELNKIKYFEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WP45</accession>
    <name>A0A8S1WP45_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphodiesterase</fullName>
            <ecNumber evidence="5">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0960150</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="5">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDO010000096">
        <property type="protein sequence ID" value="CAD8190497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WP45"/>
    <dbReference type="OrthoDB" id="342865at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="PDEase" evidence="7">
        <location>
            <begin position="278"/>
            <end position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8190497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="607" mass="70664" checksum="C7108226A0BC769D" modified="2022-10-12" version="1">MSEQQSLKDDRNLPLATAKVVPRGSKDISNAIGDRRSSYNIEGYDRVIAYEITQQYHQGIPLIDKAVIALVVIFTLLVFINFSFTSNDAKEDPNIDKTLFVTKIIELVILILFVLEISIRSYTNGFIVYFSDCWSFFDALIIIASIVLIILDLNLQGDAFITISKVLRGIFRFLRLFLVFRKYNQVKKINNSGSRYTVRSPVEKVIEIMRDLSDQFEDPEIIKQLNWGITHISNNTVYEPIIEGRKSEALGWLNQPQNQQIISQDSKRSISTDIIFSNETNLPESLRQDFSQNILKLDYDYFSLFEKYDSSILTYLMCYYFEKEQLFSNLKISPDSFKKCVDQLGSSYHRENLYHNIIHAFDVTHTVYFFIEKCNFKEIGKLTKLDYSILLLSAAAHDVDHPGLNNIFLNNTRHELAMTYNDKSSLEQHHAATLFKYIRDTELLINFSLQDFKYFREKSISMILSTDNAMHGKDYNRLKARLASNDFDPGSKDKGICFDTLLHAADISNPFKPMKNYEKWTFRVLGEFWQQGDKEKDLGLPITMLCDRRTTNVAKSQIGFIDFMVLPYYNTLQQILPQLAEFMEQISENKRFWAEQIEHYQMLLNTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WQ75</accession>
    <name>A0A8S1WQ75_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cystinosin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0960174</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000096">
        <property type="protein sequence ID" value="CAD8190545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WQ75"/>
    <dbReference type="OrthoDB" id="75720at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015184">
        <property type="term" value="F:L-cystine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005282">
        <property type="entry name" value="LC_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13131">
        <property type="entry name" value="CYSTINOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13131:SF5">
        <property type="entry name" value="CYSTINOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="132"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8190545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="279" mass="32445" checksum="B108ADAC9F550DE2" modified="2022-10-12" version="1">MLSLLIVESEFSRDSSQEPNSIALDILSNFIGWTYIFCWSFSFYGQLYENYKVKNVKGIAFEFLGLNLTGYLFLSAYSSAGYYKGGDSGWPFSGNITLQDLIFAFHSVGITILTILQIAYYYKEGDNPGVSLWCIIVLITLWSSTILYIVLTWIFGWEVIFKQEKLNVLYWMGYEKLFVTLIKNIPQVYLNYKRKSTVGWSIFNILLAFFGGILSFLQMLIDQINGKSANLVDMNIVKFILNWIVLGFDLIFILQHYLLYHPKRNKTKDIKDEYQQMKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WQA9</accession>
    <name>A0A8S1WQA9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Arrestin C-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0960073</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000096">
        <property type="protein sequence ID" value="CAD8190349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WQA9"/>
    <dbReference type="OrthoDB" id="288310at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin-like N-terminal" evidence="1">
        <location>
            <begin position="5"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="2">
        <location>
            <begin position="169"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8190349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="326" mass="38090" checksum="9BE06A11174C70AB" modified="2022-10-12" version="1">MGNKIQIQLNSKFYFPNQAIQGMVIIEDCEYTLKEISKLYIHLQGEETWEKGFACIESNNFNIFYSQFKLLIDINDERNQEDQKLKIQKTIAIPFTYQLPENIPGSMKREKDFKLRIDYNMSLIAETLKLDRITLNKLPIIINQYAVRQNIIKTISNSHIIRGYLNKHQGISKLECKYSGEEFSFGDSIHLEMIVDLRESKQYIQEIVISLNKRTTIIAKEPKVIIEQISKLSLPGVVQGIVQTIKKSILLFDFHNKMELSTTGQLIKVDYLIRIQAYSTLFNINNNDLRIDIPINIFQRIIKLPDLKIESNQIKDLQQLYDVVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WS41</accession>
    <name>A0A8S1WS41_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1020037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000102">
        <property type="protein sequence ID" value="CAD8192553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WS41"/>
    <dbReference type="OrthoDB" id="304156at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="641"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="645"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="731"/>
            <end position="749"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8192553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="749" mass="88058" checksum="CDF2703A2ED5D512" modified="2022-10-12" version="1">MMDTRQLEAVPVQSRNTVKQQEDLYISLPDLYIRKYVLSEKICALKKPYIPKVNMHDQTNQEILRVPKVWQQVMNDVQLKALDFSERKKWKIAVNYQLAQEAKANHQMLQKEREEIEQFGICISYSLCQMVQKAFNQMDIEEPNQKPIQKYLRIMKSDNQELDEQRYQDALQKVKRYLSTNRDIYVSLESLFPDMQQTYSESNTHDIHSNDRRKIKYTVEFDFKKKYENLQEALQLFVNKDIPLKESFEKSMMSIESFPTSLDMIYKHKAKSYYQEPCLDPEYEELLYRIGCTPEQVLNDGEKMMKKMKIFSSNQVVEQQLLPQLELKDLFRYKYNYIEKDCHFIQQNGKFIPLNAERFNSHAQSITKSNELVKQYFIYYYQKFQGDFNQVSQALSSNTVTANLYLYTPNFCADLYAWLIFNKSIPSNSKFILPQQYQKKCESIKQIQKSQQQQTQYTNILNDYEQLIQKKLPTKIQHLRSLPQIPFKQYNKSVFKELLTKIKFDDPIQIKTSGQADYSMVERLSFSNLKELVKSVNNSKGHIEKRYKNIYDIARQDCYPIEQIKKEFRKNFKQQIQVGQRMFPQTMKYNNQPPNSGLQINQYQSKPTTIEGINSTQNIIMPQQQPPPQSAMPIQTQLPLQSALPPQVAQTPPTAQQYIPPATSRPRKTKQEVPAQPPQQAPPTVQQPASAKKNNRKKDDTVVQPASVTTPANVEPQEPPKQSKRTKKQTKANDKNKQQQPEDRKDDSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WS48</accession>
    <name>A0A8S1WS48_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0980041</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000098">
        <property type="protein sequence ID" value="CAD8191029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WS48"/>
    <dbReference type="OrthoDB" id="283959at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8191029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="376" mass="44115" checksum="64F21471FE216687" modified="2022-10-12" version="1">MALIHNQNDIQNMFSKAGKITSEKYIVFQRKEEIINNRPRYFKTKSQSVASPEEVNLQPEKKIPEIANKKQVQITEAELIQQKNQLKLSQPFSKQLSRQVGHYTKLSNDNKPSCGQYHVKFSELDKAVHSIHDYEKSLKKTTPRDVEGKFGQPESIDKPIVDRPRFSRTCFTDLTRQTQRPDIFFGRPTPHPDRFTFLSVTDSWSKIPRTPNVQLDKQLSREQMIIYKKKQFAPDYHPNFEYGRKQLGSCGAAFDKLEKRKDIMTKIPPYNNEAYFEFDVYSKDPQSKLFRNPTAPNFDKMLERECDGKSLLPSFMQKYTNTRMGIAHLNQKMLEVNNFRDGRFQTVTSSFLPSKFKRKQKLDESSEEIEQFEDQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WUR8</accession>
    <name>A0A8S1WUR8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1000050</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000100">
        <property type="protein sequence ID" value="CAD8191869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WUR8"/>
    <dbReference type="OrthoDB" id="304646at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001335">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF30">
        <property type="entry name" value="MATERNAL EMBRYONIC LEUCINE ZIPPER KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="655"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="683"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="793"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="100"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="427"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="490"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="529"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="427"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="497"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="529"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8191869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="812" mass="95097" checksum="63AD8A90457AAFAF" modified="2022-10-12" version="1">MDFELNDSLQGDKKRHILLTLNGQADAEIIKFRFFQGQITMHDIKKKMEKHFKMKISWLRLYTQQGVEIFEEDLLFIKEGTLLYVSDSSEFDAYSQISVYQIMKVLGEGGFGKVMLGKHKISGEQVAIKLIDSGKLWNAEDIDLVFREAEVMKNLRHNNIVKILNCYTLPNMQVVLIMEFLQGGDLVEYLQEKGGLSEQEARIIFRQIAEAIRYCHDKRLIHRDLKLENILLTSKIEKIIKIIDFGIATVSTNFTIDKVDRGSLSYMPPELMSGQAIEIKPSIDIWALGVILYALVCGNLPFTSKNDEQTIDNILKCNYSFPPSLTLTKEYKDLIINMLNPDQNQRYSAYQVIRHPWMQKVVTQSPPNLLSPKVIATKKMIKKEVTMAGGPNRNQQLQLRGGVTMMRPAVQSNQNSAVIPIIYEFQTRNQQDPQSPRSSFLDNGTNRSVQEPLENQSQDKAVPKTQAIRKYSDFNQKLIDRIFREDSKREKVKKWKSNSPTMKGSPIKQQNQGSLKQCTSCHFNSTRTTNTSPYTISRSTLHTPNLISPRQSSENKKRSYDKLEIQTIINLSQRVEWKQLYSNQVTPQNNIQSRRNTMSASKADMLYKLQIQFKQRQKNQDIQQQKKLYENNKEMTIQKLCNIIFSSRIRKVSHFYYQIISTSISVMFDMLMTFIVYDEVITYWQIVLNCILFYKFSYQVMLIYVLIMKKDSCFLQIVILVFLRSYRSSFKIKFFIFIYQIKIKDYEFQIQIHFVTDATFIKHHFNKIEVVKLMWIILIVFQVLQLRLLVQRFFYYSFSVMFLFSYIQSKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WVM3</accession>
    <name>A0A8S1WVM3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1010107</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000101">
        <property type="protein sequence ID" value="CAD8192355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WVM3"/>
    <dbReference type="OrthoDB" id="304131at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8192355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="103" mass="12253" checksum="41A9C14F3868EE88" modified="2022-10-12" version="1">MENQLKIFQEELQELLIKSQLNMRKLTINNIQLSDLMKTSANDLHQAVEENTDKVNMQIKQCMQMQDEFLQIEALQKKVEIFRLIVEDVQKKKEQEYQSLLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WVU6</accession>
    <name>A0A8S1WVU6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1010135</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000101">
        <property type="protein sequence ID" value="CAD8192405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WVU6"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8192405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="62" mass="7292" checksum="DB7651F35CBEFAC5" modified="2022-10-12" version="1">MLLYGTLIPAIFLQIQNQQILFKQPPDQLLIIYKSIQIKNLFLKDCQNSLAKVKHYYISILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WZ38</accession>
    <name>A0A8S1WZ38_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ABC-type uncharacterized transport system domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1070094</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000107">
        <property type="protein sequence ID" value="CAD8194652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WZ38"/>
    <dbReference type="OrthoDB" id="10259368at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030992">
        <property type="term" value="C:intraciliary transport particle B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23683">
        <property type="entry name" value="IFT52_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039975">
        <property type="entry name" value="IFT52"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055460">
        <property type="entry name" value="IFT52_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055458">
        <property type="entry name" value="IFT52_GIFT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048643">
        <property type="entry name" value="Itf52_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12969:SF7">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 52 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12969">
        <property type="entry name" value="NGD5/OSM-6/IFT52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23352">
        <property type="entry name" value="IFT52_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23355">
        <property type="entry name" value="IFT52_GIFT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21178">
        <property type="entry name" value="Itf52_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IFT52 GIFT" evidence="3">
        <location>
            <begin position="6"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="IFT52 central" evidence="2">
        <location>
            <begin position="275"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Intraflagellar transport protein 52 C-terminal" evidence="1">
        <location>
            <begin position="366"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8194652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="428" mass="48950" checksum="FB52DB12C0863BAE" modified="2022-10-12" version="1">MSEPQVIFNVCKNEQGNPSQNYKKILKKYKGQYKFGLNKDDLVIEKIRNANLLIFGGSRAPFTEKEIKCLEEYFINGGNLLILFSDGGESKSTSNLNAFIEKFGIYVNNDCVVRTCYFKYFHPKEAFIQQGVINQEIVRVINGEQRETRKNKASTPYLQGVIEMEDTEINNQSGLDFVYPNGATLIVKDKAFPILSTGPMSYPCEKPIMAINQEKGKLVVIGSTDIFNDEYFEKDDNQKIFDFLLKFFFTKEVEFDKKTPFVENDCKNAPDIAELSDKLKSCLQESEELPKDVTTLFDSTLFKFDLDLIPDAVKLYETLQVKHEPLTLIVPQFETPLLGLQPAVFPPIPRELPPPPLELFDLDEEFASEKTRLAQLTNKCTNEDLEYYVKEAGDIIGITDKLKNRQKAQSVIHYVLENLINFKKLNQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WZQ0</accession>
    <name>A0A8S1WZQ0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0990048</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000099">
        <property type="protein sequence ID" value="CAD8191466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WZQ0"/>
    <dbReference type="OrthoDB" id="809632at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05288">
        <property type="entry name" value="PGDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041694">
        <property type="entry name" value="ADH_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045010">
        <property type="entry name" value="MDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205:SF42">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE, ZINC-CONTAINING (AFU_ORTHOLOGUE AFUA_7G04530)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16884">
        <property type="entry name" value="ADH_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidoreductase N-terminal" evidence="3">
        <location>
            <begin position="4"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="2">
        <location>
            <begin position="168"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8191466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="359" mass="39594" checksum="8EEDE406544E0AE3" modified="2022-10-12" version="1">MRGVTLRRYPDESFPKNIDFFDLKDFPIPTNLKENDLLIKVCYVSIDPVMRVWFSGAKTYIDTVHQGQVIPAFGCGVVAESKSKRFNKGDLVLGALDCADYCVRSANTIFKLPAVKSKNDPNLPLFLSVYGVTGITALKGLHQIPKENLPTKESKKTLVVSSAAGSTGSFALQIGKYWGYRTVGIVSSRDKKDFVLSLGADACVCYNDTLDSNGIVDTDKLTKLIKQAAPEGIDAMYDNSGEEVLDAVLPAMNKGAFIVLCGATATYYTWKNRGGLKNLSHFITKQIKAEGILYFGQKNAIMESFKEMNELVMGKHIQHKEEFINGLENVTLGLQKVFLGKNLGRVIVKLDNEVPLPKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X330</accession>
    <name>A0A8S1X330_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1080049</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000108">
        <property type="protein sequence ID" value="CAD8194949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X330"/>
    <dbReference type="OrthoDB" id="20524at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24362:SF309">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24362">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8194949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="259" mass="30403" checksum="F346BCA66338E775" modified="2022-10-12" version="1">MNTQQIQKQLVAKIYKNKKIPDDGEILKLKQLNHPNIVQIYDVYENEKDVIVIMEKCQSSLKNELKLKKEYAEIELLCFLSQILKGYNYLKNQGLEINELKPSNIMIDDNEMIKLSNYGMKYLYNYSVVDIRPYAAPEVFFSNLQTDAKNIYSVIMCQDIIQLGLIMYQLIFQQLPFSLKQNGDVIVFLQKIKKTKLSIPKNKFQKLTDMITEMIVYDHEVRIDIEKLGNILFQNFKRGSRRISSIQPIAAKADDYLYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X527</accession>
    <name>A0A8S1X527_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1100081</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000110">
        <property type="protein sequence ID" value="CAD8195699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X527"/>
    <dbReference type="OrthoDB" id="2021138at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="234"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="281"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="338"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="383"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="413"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="8">
        <location>
            <begin position="519"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="755"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="433"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="760"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="781"/>
            <end position="795"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8195699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="964" mass="114003" checksum="3E8454DFA3ABF140" modified="2022-10-12" version="1">MQIITNYDSEPFQLINTKRQIFGRDSDIKMRQYFERSPSIEGMDEMIDDQDKSAKPSLFSLSKANSNYKPPTINIQNIITDQRETYIFENLNQDQKEPGFNPVWKHGSLKIIRLVARFLYQLKTKAETLKLKLMNHHIFKYIDDKASDFNEFKSEKTIDDHSFKNIMCRNLKKMEGAFSVIKAILEYLFNRIGVIYPESTFKIIWDSIVVCFIVINIFFIPMSLSFELDKSSTLVWLFFETIPSYIFIAEILLNFNTAYYGHGVIHSTRKEIFHHYVSENFWWDLMISIPYVLSQLDIPYIQFVLLLRITRVKSMVQNVEDLLNAKESVQAVVELSKLIYFIIFVAHMCSCAWHLLGKIEVDVYGDNNSWLIHYGYYDKPWQTRYIVSIYWSVITTLTVGYGDIVPQTSIEQLFVVIIAMIICGVFGYSISTIGEILKNLEEKKGQFKQLMKEVNSYIKEKELNLQLSLKVRKYFEFYYQTQQSQKRSYQTLINNLNEQLKQEIMIDLYKKILIQSKFINDTFNEILINQLCQKVQQENFGPGDEIIDIKNKSEKKLIFVLNGQVDSYFHIQRTQKETLHSITQGKFSRTYKKGDIIGEFEFITNNDYSVQYRAFKFTQVAFINRQDLLEIITKDKEHLYKFQTLIDSLTYSQKLGRTCEICKWTHLYQNCPFTFYQPNIWKIVRQASASVNIKRLSFERKSRQRYKAISNSQMTIATILDFMILHNFIQEDLLCSKTLKRLGFKNNHRISQREIHEISSESSKTSSSNESQSLVIDNHKNVQMKNKRTSLQSQNDKNDNKFDRLRTTLNMKDSNKNNLIKPILQKMNSFGQQSLEDTNNQNSIPNIKLFPQFYKKKSSIQNIKPMLKLETQVNEVDTNKDTLKLYSNQKIPENFDDMDLMMQENNNFYPEFNIFKVIETYNKCRIRNLSFRKKRLFHEKLKSIRLQKQFNLKILTPRSSQQII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X955</accession>
    <name>A0A8S1X955_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1150159</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000115">
        <property type="protein sequence ID" value="CAD8197540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X955"/>
    <dbReference type="OrthoDB" id="320054at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8197540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="172" mass="20121" checksum="4FD82D5024795F9A" modified="2022-10-12" version="1">MEKTVIIIIMTYFQTNIFVKASLLRLCLLLYQLYAVKQKPHILNNLNELDVSTGQICSIATFLATIKYISEFQNKSTSVVIEMILVLLCLKLSYPFVIDLLRVYYKKYKILLLSNLYDALKIINCNLSLTLYINRKLNLMKQREQKLKTNLIKLGIHLLQMSKFKQNHKNLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X9U3</accession>
    <name>A0A8S1X9U3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1160167</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000116">
        <property type="protein sequence ID" value="CAD8197896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X9U3"/>
    <dbReference type="OrthoDB" id="292317at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8197896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="665" mass="78965" checksum="CD79A83A61E87EAF" modified="2022-10-12" version="1">MIKYFFGSIKLFRKQGIQDATKATTTKLDQITNPELTKDDIANFDQFYSNIVKSMVVKENGRKKVIQQTYTPNIYQILEQKIGNDHFQISAFLQFRRDQLREEEVVYCLDGLIRAKQFIQLKNSDYLQHIDSLISNKKLQFTKIECLEQILIINKQLQFLSIDSLRLIAKQLNEIGQYIEKEYELTKNEEPLEMLAQAFILAAQIEYNHTITQSWLIHVRKILKRHFKLLKFDTILKYFITIIKYQNKLQTELDSILDIEEFFGENYNVLTLENYLIIIEHLALIKYKKRSEVVIQFINNINSFTFTIQQLAQLAKSLAIMEIRLDELWKRITSEFLQVIQLVDAHFYLGEITLIFQSLLKINPLSPQDLTTIYKTIKNISIDESYTIKFQILYNFSIKGHFNFDLFQDLCNYEKNNFQFKYLNSYKQEKLFEVIKVILYNSIFYNKLECLDEKVDIAIQKALIILCHKIYEILSNPNYELKNSFQTQYELFIVSSLFQKYIENSDGLQETLINNKLENIRQDAGKKLDRKIQRNQFQIQEALKLLGFIDYFKVGYKEEAYQELVRMVQVKLIEYESDFDELNKLLHQYHIQNQQIMQEYTSLILNKQAELTIEQLQKYSHVLKKSGNQSQDLELIIIQRSLTNNSYLGVVGLDKPNYEVNFLFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XBP0</accession>
    <name>A0A8S1XBP0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EML-like second beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1190010</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000119">
        <property type="protein sequence ID" value="CAD8198460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XBP0"/>
    <dbReference type="OrthoDB" id="1850764at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="408"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="450"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="459"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="492"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="534"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="576"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="618"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="660"/>
            <end position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8198460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="705" mass="81806" checksum="FC6CEB4AC82327C7" modified="2022-10-12" version="1">MNCTHHIQCPINFICIAPHKCQYQRKLCVQCINEHRVDIQHIVEINKFREMVIQKIKDYQFDKASELTKQRNNFISVLSQIEGILKKIWKQLTESIQQLYDMFEIQNKSYLNIINENTNLIEQHRTQLDELVLIFKGQKFQDWNAYQNSFLIKLENVKNWWGHEVEDFNERLKKQIKVFFPFNQQEFKQLSMLQPIEQKEDLCEILASIKYIDVQIFRLIIQMLKQEKITDFIRFLTIDENQKYFEKYIYQEKSHVSPFDKNKKLKDGKVSFQLVTKIIKQINEDDFNQNDYSSEIYNETKKALMNKISNEEKILEFLIFLIYLSTLDKKFMQSGENSLNLLIEMKVDLKQLGQKNIQTESMSIIQNLVEQQNQLELDQIYISEVNFLRKQILNYYLIDDSTQYLLKLQGHSEEVRQTCFSSDGTTIASCSDDQTIRLWDVQTGKQKSQIEGNCGQIKSVCFSPNDTILASGSDDQTIRLWDVKTGKQKQIFKGHTDQVRSVCYSPDGNTLASGSADKSIFLWDVNKKKLISKLGGHNGPVNSVCFSPDGNILASSSADKSIVLWEFKIGQQKYKLNGHQKSVFQVCFSPDGMTLASGSADKSIILWDVRTGKEKSILIGHCDVVRSVCFSPDGLALASGSYDQVIYIWDAKTGKCKSIFRQHYREVLTVCFSPDSTILASCSGDKFIHLWNALYPQDQSSFYLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XHM9</accession>
    <name>A0A8S1XHM9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1240030</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000124">
        <property type="protein sequence ID" value="CAD8200258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XHM9"/>
    <dbReference type="OrthoDB" id="292239at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="138"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8200258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="584" mass="68249" checksum="55ADD7B96D83A3F4" modified="2022-10-12" version="1">MQVKLSFFDDAKFSKSFWEKTNIQIEEQKIEFQSVLFEKSKKTGFWTSKNYIIQDGKLIKLRPNKKNIHHVNLGISRIERIKYKNEQESDVCNKKKYGFRIIRNQKFKELYSRSKELNQQIWDFLKKQCLQLTFKEEYVIERMIGKGNFAKVYLCNKKSDKLQYAVKAFEKSKMMNSETDKQALIKEMSIMRKLNYKGLIKMHEIFEDDTHIFLVQDYLQGGELYQQIKKNQKQSEPIVATILATLLDSLDYLHKQNILHRDLKPENMILRKKGVLEDVVIADFGLADFYDPLGNYMFQRCGTPGFVAPEVLQDKIYDSKVDIFSVGCLMFLLIAGKSPFKGSTYDEVVMRNYHCKIDYPSIDTIISSAGLQLLKLLLHPRPSQRPQPRDALRHEWFMINLDKTRYSELNKSEEYIYDTKDTIKSSNTDAGSCGFMKNFIGPSNLDKPEFMTPQQNCIGNKKKDNLLFTPQITVMQQIQEQFNDSEEQMSIKNLDDDFTDMIFDDESPQSHRLPQYQLVGKLKQAVDSNNSSYYASPIIKKTPESLAKDINPKVMTIKQKIYEQQTQPKIASKIKNNLKNLEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XJG5</accession>
    <name>A0A8S1XJG5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1260102</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000126">
        <property type="protein sequence ID" value="CAD8201009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XJG5"/>
    <dbReference type="OrthoDB" id="10381304at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="402"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8201009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="561" mass="66608" checksum="D9ECFF24C4EABC3A" modified="2022-10-12" version="1">MNKQSSNYFISRCLRAKLGKFKMRTQILIINGIILSLLIPTILILQTVNMQYVEQILQHSLKRNTIQEISKHLNITSHLLNYQLNSVFEKTEITLTNLNQLYWIQLKSDIIATSQQPRMCDYYQPTMPQEHYYSILCFSRFGQEQNSSTNSNINQSLESITNLVNSFILSLMRLNDEYLPNQMYFVSSINSNQYGFLYPQFTIYPNFNPKEREWYKSHFKNLEKDKTNHTQLTNIYRYFDTQPIYSMTMTQSMLNLNKEVEGLFCSDLVFKNNLIPQLNINIMIVNTEGTLILSNYKNDIVSNSSALIKFDNEEITGFKTQDWNSILNYYYNKQLNSTCNYENLHILCRYNSIYNKDVVIQIIKLQNIDYFLILFYDIQIEQEIDDNINLLLKIVKKNLNQIIFVTVLGSFGLILLQVILIYIIFLPMYQIIRQSYFFLKKSEKSKQFEQQSLLQKINHFQEIIINQSITNDSQVNNNALMQFKFQFNTLFDRLLAQRMTINPQCQILQQFKYPRKGIHNSLDILKILKEDKIYDNYYDQQINSPKNCHLRNPFSFRVEQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XMJ6</accession>
    <name>A0A8S1XMJ6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1290114</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000129">
        <property type="protein sequence ID" value="CAD8201948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XMJ6"/>
    <dbReference type="OrthoDB" id="291229at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8201948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="452" mass="53540" checksum="03B590CCC8C5E26A" modified="2022-10-12" version="1">MLKLKREYFRNSIQKLAREEIFKKKRIVTNNNYNHESNPFNEKLNQLFESELTISDIAKQLEQLYEEYKLFPERNTLVLAQIKILSMKVATMKIQEHINDFYNSIKNNILPELIKYCSQTYADDVNTVELTNNSLWILINLTLADDCVNYLLDYGNGYFLEQINFLIKSKYEVIVHRVFELIFNMTVEEQACDRILLDSNFNEAFLHRLKRQNSEIIMNLAASIIKNFCNNFRNENLQKILDFIFYCIRFESVNGLSYMITDEYCAQFLTFYRGLCLKLFTHPNHSINFINYLSSHSSNEVLDLLLDYPIVDELIRIKNSNHQVKVYSTFSNLATHQPFHNELRVLFQDIYEQYEKSDLKMKLELGYFVSTYIINCDPTFYGDLINNGVINLLVDFFREIYDPKAIYLALQAVLHLFMNENSKQIFEKLGGFKQLEKLRQNDNIIELASRLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XNK1</accession>
    <name>A0A8S1XNK1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1310116</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="EMBL" id="CAJJDO010000131">
        <property type="protein sequence ID" value="CAD8202593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XNK1"/>
    <dbReference type="OrthoDB" id="9909019at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="181"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="8">
        <location>
            <begin position="96"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8202593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="283" mass="33476" checksum="77D1DEC460B023CD" modified="2022-10-12" version="1">MVVPNEVKDIGIACKRFLFTTEFSGFLILTLFQNELILSIGIRLLIEAILAAYFYIKCSDSSGIPLQGDDDLELSQLSISTQQRDNSQQESQIPIRNICQECKIIQPFRTKHCTKCKKCIPKYDHHCFWVGGCIGELNHRIFWLFLFFQCLLCFDSLFQFNKQLSLYSIYEEEQKDNKYQYQYFVILLFGAISFGFGIFTGALLLYHTMLIVTGQTTWEHTKRDKITYLKFYPKFYHPYNYGLIVNMRLTFFHNNKQSYWIPPQKDQIQEQCNLFDNKYYSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XP69</accession>
    <name>A0A8S1XP69_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1300163</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000130">
        <property type="protein sequence ID" value="CAD8202314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XP69"/>
    <dbReference type="OrthoDB" id="307108at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8202314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="121" mass="13789" checksum="6BDC036E40CE79F0" modified="2022-10-12" version="1">MKSSKSISSNKTTTSSTSFILNTIPISRKHNNQPSIQPLQKSTSIMKKPYIMISPKLIQEFDENNTQRTKLLFENRLSLLQTNVQSKSESQIPNTEGQTLTSRVRKRTAPIRLSMRLPTQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XPC5</accession>
    <name>A0A8S1XPC5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1330033</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000133">
        <property type="protein sequence ID" value="CAD8203109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XPC5"/>
    <dbReference type="OrthoDB" id="285381at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8203109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="369" mass="43137" checksum="02288CC6D77BD6DE" modified="2022-10-12" version="1">MKSFQCTDHKKDIVYYCNDQHCEMRCLCAECEHEHDKTAVQNLLEGYNIKELVQTFQSFKCIINDLMTKMEQELSQHLQQIKQPQATAQNLEQFDKQLSEISNIQNTLKNKIKNRLQHIINALKGQDLDQSPNVTIFDARKKEHSKSVILSQAQIQQDMIKKTRQSKIFKMNIPQSNQQETQQIIQQPNLNVDRIEVARLQQLSTQDWAWKMTSSTVFCCIFSSLRDLKIIGFLQPCLFKSNTNNYQKKSAVINVGIYNKKNLTKQPIHLEKYTLRHSKLKIVNDCYELMFSKPIAIKEFTEYSIAIWPSKVLYSHYFSIAASINPFIAFQTQDFNDNPKIKRSEEFPQVISTFRAGLVPYLIIDPRQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XSV1</accession>
    <name>A0A8S1XSV1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RIIa domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1360049</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
            <location evidence="1">Flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ropporin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000136">
        <property type="protein sequence ID" value="CAD8204145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XSV1"/>
    <dbReference type="OrthoDB" id="10067602at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd22985">
        <property type="entry name" value="DD_CrRSP11-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.890.10">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003117">
        <property type="entry name" value="cAMP_dep_PK_reg_su_I/II_a/b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14952:SF9">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14952">
        <property type="entry name" value="ROPPORIN-1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02197">
        <property type="entry name" value="RIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00394">
        <property type="entry name" value="RIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47391">
        <property type="entry name" value="Dimerization-anchoring domain of cAMP-dependent PK regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RIIa" evidence="5">
        <location>
            <begin position="19"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8204145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="67" mass="7886" checksum="D1A2D126D82D976D" modified="2022-10-12" version="1">MADVREQRIYCAEQIVVPPELPVILKHYAKEVIRNKPGDVVDFSAKYFRSLLEKRAKEHEFSEIVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XT44</accession>
    <name>A0A8S1XT44_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1340142</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000134">
        <property type="protein sequence ID" value="CAD8203722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="409374at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035922187" evidence="5">
        <location>
            <begin position="17"/>
            <end position="1475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1198"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1307"/>
            <end position="1328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1396"/>
            <end position="1417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1429"/>
            <end position="1455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8203722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1475" mass="171729" checksum="29CD9E3B99FBF4F9" modified="2022-10-12" version="1">MILLILLVYKTYQTLIYQFHANSNIRDEWQDYYSNVNFYTCGGIQYFGQPNNNGNSIVISRIFLDLEPHSHIIVDAQFLTIDNNQSPYFYIDQQSISYQSVISSQLCGNTQFEKVYTISITHQHNRRTVWIYIINYYAGGLISLKLSMIKCQYECAGCIENYHTFCLQWKLHQYSFNQKLITNSDGWTFVSSYSYLTYSLCGNCQFLYFQQIKYSTELPPHQDVLIRFFKLWNTIIIVDYLYGKQIISSNNQQIEILIRNHDDPILLLNIKTQLASDYSYIRDFEVFYTQAEIMFNNLNEGCLYQIDDKCLICQEGWIQDEFLENCHPICGDGIIQGQEQCDHGNLISNHSCYLCQYSCQQFCSICQIGICQQCQDGFVINPNFNCDPLCGDGNLIPYSNEQCELTVNGVLDGCQECRYISIENCKISYNSICLKCEVGFYIQENLCFPYCGDKLILELYEDCDDGNLQPYDGCFNCKFQCIEDCNICERGQCLLKCEVGYEFINNSCLSVCGDQIITKEEDCDDGNLQPHDGCFNCKYSCPKNCFDCYQGTCLECNYQYQLISNQCKQQLNCGDGLLQEEEDCDDGNYQAADGCKDCRIEQNWICTTRDSLSLCTFVKAPNLVINYLNMTQNKQYISIQFNQKVKIYTAQPLSETINFEISNIDKKNWNSSLYIIQDVGSDVSFGEFIFQIEIQQLLEFRPVLKIKVSCQQFCSICQIGICQQCQDGFVINPNFNCDPLCGDGNLIPYSNEQCELTVNGVLDGCQECRYISIENCKISYNSICLKCEVGFYIQENLCFPYCGDKLILELYEDCDDGNLQPYDGCFNCKFQCIEDCNICERGQCLLKCEVGYEFINNSCLSVCGDQIITKEEDCDDGNLQPHDGCFNCKYSCPKNCFDCYQGTCLECNYQYQLISNQCKQQLNCGDGLLQEEEDCDDGNYQAADGCKDCRIEQNWICTTRDSLSLCTFVKAPNLVINYLNMTQNKQYISIQFNQKVKIYTAQPLSETINFEISNIDKKNWNSSLYIIQDVGSDVSFGEFIFQIEIQQLLEFRPVLKIKVNQTVANIDDAILDNVEKSITLQYPKFLDETQKDYSQNLKNLNKYIIYCLSGVTGLSLLLGNGDLFVELMAILQFQQYLRYINLQYPQNIEIYFTFNDMITIQPLLDFIYFPKLLKFIDIQQNQAYSDGKFNVYKQSYNLIINLSCQIFQLLIFLVFILLFQWIKKGLYKWIFCSRYFYYMSSLSLYINPKVIFKFSQSFYNICMELLKLKKFMSFQGLQKAIFLNGWDMIFKTLLYTRNIETKNYIDIVQIIIASIILILYFTIFLSFFKGNSKLSKKNNYQMFEILSFVRQFFFLLFLIYVQSSQILQLGLLLMTSILQIRFLFNYRCIFNQKNYIVQMVVEISVLTFILSSFLYIQEFNKYFNEEKKILLGWIQAIILSKGIIIELIWISIDLLQKIKKNNRRQSQIVKNPLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XVT1</accession>
    <name>A0A8S1XVT1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1410043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000141">
        <property type="protein sequence ID" value="CAD8205579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XVT1"/>
    <dbReference type="OrthoDB" id="300617at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="709"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="808"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="241"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="816"/>
            <end position="837"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8205579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="898" mass="103579" checksum="6119DF00DAFD9ED5" modified="2022-10-12" version="1">MNQQISHQTKTSQTQQFIASQLSKLPDKTKLQQLIPVQLHVNCFERKHNEGIDNRQSPPQQQNYCTKPSQKPQQSNHKSNMSLQNKSNSHIESIQEKLSAINHPNKKRDYSPNSDAIRQLLKNTKQDSDINNLLSKRQQSQDQQIILTPITAKYPLKSHSPLDIKESVAQLLQNHFKTDVSSPKCNGSNQKQKNNHLGPYLSIQQQIQQQKLKGHSSTNSLSYASRNHHQQSLQDSKKSETATITPTPKKFTTTSQNTEHSKSKSVVEEQLQCMKIMQFDKENQSHILNLGSPSAFSSSEQASSIGDSIGMHLQFGVKTQSPFAKQPQSTQKYCIYHKEKKAKYVTEEGNDYLFFCSKCAVKLAGKGMFFTEIAQLKQSTVDSQTLSFQQSSLDSMNRQSSSVDIQFKEREMKVFLALLYQIQNNRQDLLNQLSSQGNKLQNYYDKQMKLCKEAKQQLSIFLEEIYQRSINQLSNSKQQTLSFIAKIFQQMTTNQLDTKKIQTEIESNWKRVLENMDMKVFREVMNQHHSKFSKIGEFQQEIQNQYIQLYSMGSMDIQSTMSLISQNFEIVESAIPLISNIQQINTTQPIRTQPTPPKKNEEQISQRIKTDFSNQKKNSDIKQQNFCQYFNEKIDTNSKDDQKLNPSFSSHNNNLYVSFENKEIFMNALEIEKNKQVQCTEDDNSSKQQAIMILQSYKGSHADIQEESLNMSHESQKSAPHSPASGQTKESVQRIIPNQEQEFVSLANNQNETVPKEAFQKQKSTFRTLFENEDSQEPNIIIKKEVTAISPARSEKFKSFLNRISNSNQKVATDNPDAKKEDTSSFNQIREDNGNLKDSQDIKDSVHQINMCDDQQHLSDYDEEKEIYDTKMRYVYQGDERKEQYQKTLFSSPMFKDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XXZ3</accession>
    <name>A0A8S1XXZ3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1440024</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000144">
        <property type="protein sequence ID" value="CAD8206419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XXZ3"/>
    <dbReference type="OrthoDB" id="289314at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555:SF170">
        <property type="entry name" value="FI18122P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="3">
        <location>
            <begin position="61"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="321"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8206419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="441" mass="51971" checksum="1485403CE46D06C3" modified="2022-10-12" version="1">MQNDETNDTTTQINDIDVQSQQKETENIQQDNQQEVIEQQTLEQQQQSNEALLRQIDDDEPIYILVSNPSEVKSSSMNKYLVYTVKGKDALGNFEVQRRFNEFYTLREALIQIWPGLYIPPLPDKLVNPTGDQLLERVRLLNIFVMKMAQIKYLYYSEEFVQVFLRSTQLDVCKQIQNLPKPNIRSQIEKFKHIFELDQVQEPDSISINKINQMQRFCKQSQQLLIKLFDQAKSLSGSKRCLKELFHSFFGYGLQEFEQQILLPCLNNEKQLILTNPDAQELKSTLDYLKDQNQISLDKIQDLISTEQRDMEAFLQGFQSRESLLHTKSKVENKLREQQEQLTKVMIAKGSSFGKSQEEQRQKIELQIVESQNDVDSYKYYYQLVTHVLTMKAIDKFKKDKHESYKRIIRDLAQLEIENNQVMQEVWTRIISLAESLRVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XY85</accession>
    <name>A0A8S1XY85_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="10">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T1440084</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="10"/>
    <dbReference type="EMBL" id="CAJJDO010000144">
        <property type="protein sequence ID" value="CAD8206539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XY85"/>
    <dbReference type="OrthoDB" id="10261556at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17920">
        <property type="entry name" value="DEXHc_RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18794">
        <property type="entry name" value="SF2_C_RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001975">
        <property type="entry name" value="ATP-dependent DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001389">
        <property type="entry name" value="ATP-dependent DNA helicase RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004589">
        <property type="entry name" value="DNA_helicase_ATP-dep_RecQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032284">
        <property type="entry name" value="RecQ_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018982">
        <property type="entry name" value="RQC_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00614">
        <property type="entry name" value="recQ_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF153">
        <property type="entry name" value="RECQ-LIKE DNA HELICASE BLM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16124">
        <property type="entry name" value="RecQ_Zn_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09382">
        <property type="entry name" value="RQC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="99"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="295"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8206539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="897" mass="104819" checksum="5FDCC0FD94898550" modified="2022-10-12" version="1">MKYGDFQRLLSEEDELIQISRAQLEQLILRNQLSEVLPPQKQSSKKIVIKQAQKKQKSYQFADVHYWTITQFDWDTNVLKALKGAFGKEQFKANQKAIINCVLAGKDVFVCMPTGYGKSITFQIPAFVENGVSIVVMPLISLIFDQVHYLTSLGIPSLNISGSNRLLTAQQILDQKIKLIYTTPEKIEKSEAFKQILGDLFNRKLIKRFVIDEAHCVSKWGRDFRPDYLKLSNVRNEYPNIPIIALTATAPEEVKEDIIDVLQIKGCLYLQSSFNRPNLIYEVRQREEFKKTVQEIKEFINSTYPKQSGIIYCLTQSECQTLSQNLIIHGIGSDFYHAGLNEKERHRIHKNWLMNEVQVIVATVAFGMGIDKKDCRFVIHFQMPKSIENYYQESGRAGRDGKQAHCLLFYNNNDYKTNLYLMDTNTEMTPQMQKYNIKKLDQMQQFCYDRVSCRRVLQLSYLGENFDKTLCNQKCDNCERDDENVEKINLTKEALKILECLDKSQLTENQLIQCLKGAQDKKKSHNSQKNIEQIFGLFKHNPRNITPLLNQLRAQNALSQNVQCYNIKGNFKNKVVYLIPNSETSISNVIISIPKEKEKKSQFLDQKSDDLFKKYLSKGNNNQNNNVINLQINQNIQKKDFTFQNEINIGTEQKKLEYDKQYGYCLEDQFNDLRERLMFVRKNIYIEMTNDASNQIINIDLVLSTDDIEELCKKLPITMIELNDPFICIAQEIKKLQYMKHFIEEVAHFVDMENINKQLFEKPKQNETIFDQVFEFQKHIRLDDDVIDITNQEFIAKQKEIEELLINGQQNDQFLDELLNFIEDDEINKSPDIVIIDDDNAINYFTQDQKSQIKQQESIIEPVQQQQTFQQDLKQTSKIEGHQQPIKLKLNLKRSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XZI4</accession>
    <name>A0A8S1XZI4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1460082</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000146">
        <property type="protein sequence ID" value="CAD8207161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XZI4"/>
    <dbReference type="OrthoDB" id="290342at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8207161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="446" mass="52801" checksum="2585159A1DDF9CBB" modified="2022-10-12" version="1">MYLTADSALQLLHEKRNKFQVEIRRKRTENTFQQKRAIFVQNKDLELLAQYALEFYNRGDLQECENYLIRLNEYADSQYSANLKSINTITFLGEIATNCDYHILAIVLSIYQKIYLPECLNPYLLSQIQRFENSELIKQFVVLLHKIVKNNPLNHLVEQCDFVSYLVEISLLLDNFEQWFKIVEVLGSYIKQPSYYFKKLVVTCIKFIEYDTDLATFQIMLTMISCEKDNKNSINYLLANAGFVDLIMDKLDKNMMLSISIISKIVEESDEGTILFIQHGLLKTLSNLYFEMRENQTAFIYITANICFLKNDYVITEVLTSEIFNTILALDEQELNKNDYIYISQMLQQLIRKYSNEQLYRYLLNKTDYVYMLGHMMKQFELREVLQNAISCVIHMAHSQSEELAKLFRYKISGKPIEYVLTNIQYLFQDINIIEQVFEFLNIKYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y0R5</accession>
    <name>A0A8S1Y0R5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1470034</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000147">
        <property type="protein sequence ID" value="CAD8207353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y0R5"/>
    <dbReference type="OrthoDB" id="433924at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00024">
        <property type="entry name" value="CD_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="1">
        <location>
            <begin position="8"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8207353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="316" mass="38391" checksum="D2F05E7684A1DD46" modified="2022-10-12" version="1">MEFQQQQYDVEFVHGKRYLNSFEYYIKWVGYSKTESTWEPETAFEGISNQLITRFERCQNYKNYHTKIYKANSHIKIRVHIMNKGCQVYKKYTSENKYALLSQTNKTESNGQQNYKLLQEPNNYKNYENRNDDKEDYDFFMRRGRGRPRKSETDREFLSYKNDYKIESQKYLQKQYPLQITECSFKYGHYKTEKEFIQEMLLMIKKKEQKKKRDDEVIIYKPQASLPSGYKSQYTDLPKFNLLNQKYYDKNSKCQVSDGKIPLECIISHHLMQNKLLVFKCKDNDEQICYFDFDTLKSYYPTLLLDYLSITSIWKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y257</accession>
    <name>A0A8S1Y257_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1470136</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000147">
        <property type="protein sequence ID" value="CAD8207535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1668230at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8207535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="223" mass="26048" checksum="FCB3CE1EA4A82280" modified="2022-10-12" version="1">MLIFIYCLLPTKISHIKTIQIXFISLDYYKQGTLKNIFNKFRNHIPLNIVRKIMEQLLNGLDHLHSNGIMHRDIQPENIIVTNFDEIQIKIANFTLSVFGEQFTRFCGKKCYAAPEVVNHFIHTNKCDIYSAGVVFYELLNCKQIQRHFDPQIACIELKKQGLNDQAVDLVTQMLDIDPIRRLNIDDCLSHKFFTDEESQVSNYKYLLNKNQVNPKLSQINNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y4J9</accession>
    <name>A0A8S1Y4J9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1520027</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000152">
        <property type="protein sequence ID" value="CAD8208775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y4J9"/>
    <dbReference type="OrthoDB" id="284527at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8208775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="123" mass="14278" checksum="B6833847FB3DC6A1" modified="2022-10-12" version="1">MKQTFNHLNSVFALLGSNSTQISPMSTTDLNDSIEYYIDQKEYQTEVEIVRYLHNLGVQTKTDPLIKEQQYWKEKKLRSQNIILDTLGNTADKLVLNPSVNRLLRKNVTQKRLRSNSPLLLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y7T0</accession>
    <name>A0A8S1Y7T0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nucleolar protein 12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1540089</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000154">
        <property type="protein sequence ID" value="CAD8209373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y7T0"/>
    <dbReference type="OrthoDB" id="297361at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019186">
        <property type="entry name" value="Nucleolar_protein_12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09805">
        <property type="entry name" value="Nop25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="65"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8209373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="14012" checksum="5A04BD82CC8D695F" modified="2022-10-12" version="1">MGKKNNNRNFSTKKNQFKKISRTKTAAVFDEKERKEYLTGMFGAKQKRKEEYIKLRNEKHKKFNKEQKAQIREEKKKQIERLQNLIDFNNSLSKTQKTESIKTSNNKEVEVTTTFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YA29</accession>
    <name>A0A8S1YA29_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Calcium-dependent protein kinase 1</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="ORF">PPENT_87.1.T1510138</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cell membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Parasitophorous vacuole membrane</location>
            <topology evidence="24">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="30">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDO010000151">
        <property type="protein sequence ID" value="CAD8208692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YA29"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020002">
        <property type="term" value="C:host cell plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020005">
        <property type="term" value="C:symbiont-containing vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000398">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000199">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000695">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 26" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17" id="KW-0969">Cilium</keyword>
    <keyword evidence="15" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1032">Host cell membrane</keyword>
    <keyword evidence="16" id="KW-1043">Host membrane</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0519">Myristate</keyword>
    <keyword evidence="12 26" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0564">Palmitate</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="28">
        <location>
            <begin position="201"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="499"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="537"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="573"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="610"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="75"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="133"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00060437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00068067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="EMBL" id="CAD8208692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="637" mass="72986" checksum="5FA19474EF94758D" modified="2022-10-12" version="1">MGSTCCHKQPQPISSQAELVCDNSNLSIIKKSNHKEESPGEPPSRLPSQSFPIPQVYEEVSALNQMKNQVPALKFSNTKGTFKDERLLKDSKESFEPSEKSDQVEERNQNLGVKQAEVEQTPIEKIVQAEKLEKQNEAQQKQQQDTGTKKSPAASSKTVPRNPMDHSPSRKYSQASVGSVSVKFGVELFVNLKKQSITKVYTLGQVLGQGAFGKVWKVTHKTTGLIRAMKQIRKSELIKEDEQKMFSEMNLLKNLDHPHIVKLYELYQDSNNYYLITEYLCGGELFERIKKMNQFSERRASDLMRQILMAVVYCHEQKIVHRDLKPENVLFSGTEPEALLKIIDFGCSRKFNSQKNMTKRLGTPYYIAPEVLNHNYNEKCDVWSCGVILYILLCGYPPFTGKNENEIFEKVKTGKFKFPIEEWDSISREAKNLIQRMLQVDVNSRYSASQALNDPWIQKHAPNTQINKKVLENLAQFQAKSEFRSAIVQYIISQMTTNKELEDLQKTFQSLDKNKDGVLNKTELVEGYTKILKSKEQAEEYVEKIISKIDKNQSGVIEFNEFLMAAINEEKIIPIKKVEQAFKIFDSDGDGFISKQEIEEVMGELNADVWKLFLDETDGNNDGRISYEEFSKLILSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YAQ8</accession>
    <name>A0A8S1YAQ8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1570009</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000157">
        <property type="protein sequence ID" value="CAD8209907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YAQ8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8209907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="38" mass="4525" checksum="76C648457819B7AE" modified="2022-10-12" version="1">MTACKQECKYVNLLRTKENSEFKTYNPLNLEVILYLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YCL0</accession>
    <name>A0A8S1YCL0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1550047</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000155">
        <property type="protein sequence ID" value="CAD8209572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YCL0"/>
    <dbReference type="OrthoDB" id="313382at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="127"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8209572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="364" mass="43252" checksum="D80B8AD7E278483E" modified="2022-10-12" version="1">MHQQRRLSPAPSSPCPRIQQQSNRMQFLQPQYIVPQQPQYTQQPIVLKTEELRPVQQLNHQSRERDKENLNPKAFQSGRLLSQHDTPQHSQRSLQQIPIGAFPSSTTNEAQIKQLREEMLKMFEFSRKDFELVKTELEQSKQELVEQKKINKTFVAQIEALNQQLVQDQEITKQLKHEIEELKKTQGQQTSEIVLFSGIKQEIIIELKSYFDNQFKLQNTQQQSQPQQQITLDTYDTYIKKYEQFNKQVNDYLLNRKLPDNQYNDTIESDNSSNQIKDKESPVRFLNQEDQQIILTKEDMDRYKMSIIHENENEEDEIIYQVDENGYVMNQDGNHLVDNYGRLIQLSEKDIEYFKSKNQLDEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YDI6</accession>
    <name>A0A8S1YDI6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1620115</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000162">
        <property type="protein sequence ID" value="CAD8211248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YDI6"/>
    <dbReference type="OrthoDB" id="306809at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="271"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="6"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="281"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="305"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8211248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="322" mass="37622" checksum="A17B1CA95A1B89BD" modified="2022-10-12" version="1">MGCGASKSQLERMKSYKQKLEELEQINGQLAKQIADLRDVKNKRKPIYIIKDQEVEELTSYKEELERKFQVIEKEILKVNKQLQIQAKAEPKKKRKTTKPIDIDDIADEEEWKKNMMNDDDITIKPFDDAQLEIESDNIGKKDINKSSQSNILIMDSQQNIKKQIANADEINNQEQQFQFDSKKIIIQNDQNNNDEQSKHQQQDLYKIKVSIQEALGEDLQAYNDEIKNKLFSQDLSSQPQQQTLKNSEIEICFNEQSLEQIAQSPEKNITTVPKKSKSSQAQTQIQVQNQQQQKAEKQLYFSKQRPSGNNQQRNTKQKYQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YHY7</accession>
    <name>A0A8S1YHY7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1870002</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000187">
        <property type="protein sequence ID" value="CAD8213805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YHY7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="61" mass="7450" checksum="19FD795A9DA1D111" modified="2022-10-12" version="1">MSRATLDLFLKILFLKSYVKEQKVCIFRENDESRFALKQNRNEWKIKYLKSLNRFSTSLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YIW9</accession>
    <name>A0A8S1YIW9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1890019</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000189">
        <property type="protein sequence ID" value="CAD8213900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YIW9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="46" mass="5418" checksum="754D00C3EC1AFB00" modified="2022-10-12" version="1">MRSLLLYASIIQLLQRIMIWNMAIQKRKQLECKLLKSVQSIACLAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RXI3</accession>
    <name>A0A8S1RXI3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0010468</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RXI3"/>
    <dbReference type="OrthoDB" id="285625at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8132152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="177" mass="20700" checksum="9B0BEB3B899622EF" modified="2022-10-12" version="1">MQEKQNEIFEILQKQAEERKIMKKQEMFQLKKQKIAEIKQDKKKLTDGLVKHPYQFQPGKGVGQFFQKSDFLLPKQHKQGLERIQSAWLPTYSHSRLISATPFDTEPFPRPPSTKEKLWRPFSSQPNKLNMIQLVPHSPKDNALTPKKNSLAFDSGEKLIPMKPFLPTRRIFSAFPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S3S3</accession>
    <name>A0A8S1S3S3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0020438</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10 11">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S3S3"/>
    <dbReference type="OrthoDB" id="298092at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040053">
        <property type="entry name" value="JOSD1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006155">
        <property type="entry name" value="Josephin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13291">
        <property type="entry name" value="JOSEPHIN 1, 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13291:SF0">
        <property type="entry name" value="JOSEPHIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02099">
        <property type="entry name" value="Josephin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01246">
        <property type="entry name" value="Josephin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50957">
        <property type="entry name" value="JOSEPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Josephin" evidence="7">
        <location>
            <begin position="4"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="17"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8133504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="175" mass="21227" checksum="98B70DBEA7157EF2" modified="2022-10-12" version="1">MQQSKEYEHQTQNYRRCGLHAVNNLLQQNKYSREDFENIAEEIKKETNLSHYTYFFGNYDLNVIERILLKESYEIVWIRQNQEINEELIADPQVYGLLISLIKEISFIEKLCRWDPRHWVSIRKVIKPDNELEFYYHDSSLKAPQIKQTPDMIQELKNLQMDKAKENYILLVKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S584</accession>
    <name>A0A8S1S584_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0030150</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8134134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="312" mass="37122" checksum="1FC873560519D9D7" modified="2022-10-12" version="1">MKITSLSEHKFTIVAKQIIKIYLIFGLQNLAVQDFLIDKKWLSLIRILIVNECKVPQTRLEIRQGVLGYCRCIWRQKLHKLDLGEKVQKANTLRYRWEQLTGYSENFTKEQQIQLIESDIKSRGVKRQGLNDFYQLTGIKLYKGRFCRISSQHIKQAIQSIDEVFAKERKHRRCGKKDAEFRLKLSHLSIYILLRAKDTLTSEGFKQKNKGLDPFEQYLCQQLIEASDICERLYLMHARVFQADRVDRYQSYMKVINRNQYRKLMFYIEYLDILRKISVEQCTNDTXMNSEISSKQIKGSTQYNRITILNQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S6I0</accession>
    <name>A0A8S1S6I0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0040478</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S6I0"/>
    <dbReference type="OrthoDB" id="541276at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="186"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8135896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="240" mass="28735" checksum="873F56D9C49A9A51" modified="2022-10-12" version="1">MNSYLKELQKFDEQTSRFYFRQIMTALIHMKKHNICHLDLKPDNIVFDSDFNLKITDFGFSKQVQGSINDFFGGTKSYIAPELLDRIAYDGSKVDVFAAAQILFAMMTSCFAFRNCQEQPYNLIKQNDYAQFWNFQNNSHQTLFPQGFPDDFKDLINKMFDPDFNRRITPEECLQHPWTMGVEANLYQIQEQFNNLQERINQQLEQQKEEYKRKKQKMRDYERAAQEMRNLLQQSQQSYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S6V5</accession>
    <name>A0A8S1S6V5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Clathrin/coatomer adaptor adaptin-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0040227</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="302453at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030117">
        <property type="term" value="C:membrane coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026739">
        <property type="entry name" value="AP_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11134">
        <property type="entry name" value="ADAPTOR COMPLEX SUBUNIT BETA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="5">
        <location>
            <begin position="24"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8135445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="211" mass="24090" checksum="F7802293C2F0CEDA" modified="2022-10-12" version="1">MLFELPDKIGKAFGNGTLSIQSSKNNKLAELINSKYTEDIVEAIRMLYCQYLQNKDLTLYMSRIINLLPREDLELKKLCQILISEFAAQPNARTSKSQGDVLILATAPIFKDLTNQNTSIKLNALKTLSSLRIQELTPMLFTAIRKIQSDKNPLVRRSVALSILKMHDMCSDDVQPDDYNDIINFLLKDTHQTRLYAIXIQDIAFRVNLKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S8F6</accession>
    <name>A0A8S1S8F6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0050199</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S8F6"/>
    <dbReference type="OrthoDB" id="301587at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035922451" evidence="2">
        <location>
            <begin position="16"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="659"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="741"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="659"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="693"/>
            <end position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8136536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="958" mass="113564" checksum="7D6AE548F252CA2E" modified="2022-10-12" version="1">MFIIFLIQAIHSFNAENFLCGLRLFYDEKTFFLIDQQNENTMPYIQTCQSPHFALDIQQTYKSSPSLNNNSYFLVGFSDAYIFAGDASEIQWLSIQEFKVKQNRTTVSIHKYNGYNGYFVDKEEDGGHLLISKENLVRDFSYGKTDAVDKFEKMGFVYIISWGGKGDVLLILKRFVHLIDQSEGQEYCQKKVALISLEQIKCNLDLNFEHLTFHTPDLYHPPIHYHMGNQIINGSLDNEYFTKNYPGLQVFVAQNLNNKVLELRIPIKNMETHQQTIENPLSGLIVTIQQENRGFIHIIIFESDEGVIINYKEDQNLAYITIGMTDSKVFVFSFVTSQFTKFPSIDLIQHVFQDCVFIIQQRNNVCENIQQSCSDVSLNIFKQLQFELESDQLIKFKSNGEYQDTPFVEMFDNRIEILNEFNVLLSSEMKNREMNHGGYIGSFLRITYKNQSINSLIINEQNENLEAFYSINDNIYSHSVQNIIGQTYVLVFTSQNELSDNQCLQLFREFIDHIQKIFIIEQIENHCEAQQRYYDVKPIQYKEIHKLLKQQAELIQTVNQDQFDQNDLEYSIDEDESISKSLKTNSQQLGFVSEVSIIPEEIHLETQSNENLKENQNLTTQDSFQKENFDNENYKQQQEIQEQPKQFNNLKNQQIYQSKKEEFDQNEKSKKNLKNLQSKQKDQKPLLNNQQRSKMEQNDKEFKSRQNQQQNNEQEDQTQKREQQLQIEQNEQYQNTFHQRDQYYDKGDRYLNQQYQGRDYQRRDDYYGRRDTYYGRREDYYGRDDYYNRYSSRRGPPLTGQSVIIPKNPNDQELGRFLRDVISKLYIYSDPCDAKSTNYNPDNCEVDNYYGSSSIKRAEPRQRDFYRSAYKYDNRYEYRACITVYSKCYFKGESLQLCGHQRNIPKSQLYLDILSIRADENYIVEFYMRGRDGRSELYTLKGTQKCLKSPLRVDWLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S8P1</accession>
    <name>A0A8S1S8P1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0040209</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S8P1"/>
    <dbReference type="OrthoDB" id="302702at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="263"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8135409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="424" mass="50305" checksum="07B82ADFD5070B64" modified="2022-10-12" version="1">MEISQDTLNFLFKELCAMAFLQQKSQNELNELYQYYTMQEARLMNQFYHYQTINEQELKLVNRFIARISEAFQLMVAKRQIIRCKESKFQPSIYNCLTQTFEGKNSLICQLIQTIYGECRLQIEILTNKNQKIEVKRIHSQDNNNDEDKLQNQFYSVQVTNKKNIQNDWQVNMKNNKKLNNYYLKVFPKLYDVISNVDKSQDEQILYFQNRDQNMDRIFNQLLQQNQHIKQSLNWSLNLDQEIYNNKIEQKLQKIKLQQLIPSNQLSTQSSPTKPSQPSNKSIKQNAPTSPFKNNSINNNNLGKMPSINVKFRSISQNVPMSETNFKSQNMFLNPQQQQAQQIQQSQQYYSSETEKSALFSKIIENYKQNQFQLNKIKIEFKPKRQHCSVLKSKLMIDLIPLHNSKLKPKATRYFLQQVPSIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SC63</accession>
    <name>A0A8S1SC63_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0070214</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SC63"/>
    <dbReference type="OrthoDB" id="302768at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035804432" evidence="1">
        <location>
            <begin position="21"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8138631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="254" mass="26530" checksum="C3DF0198A28CD923" modified="2022-10-12" version="1">MKTQILLIAMISLSFQALTATKKCDCTEIVQSADCKGGCTWSSADSSCAAKTVDCATLTSQPTCDSESTCAWSDTTSKCAAFTACADYTVTTAEHCYYKNDTCIPGTAGADGKTPCKTGSVTCSSFTNASDCNGKSPSDTTMCWFKAGACSQIDVSKCSAITDQDVCSIICKWTSAGACAAYTCGDMTTAESCDTVLLDDFSGVNICTFNTSTTKCEDAVDVSALTSSNCLDKTSGYYYWDGSACSECSGSEKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SHE2</accession>
    <name>A0A8S1SHE2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0070252</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SHE2"/>
    <dbReference type="OrthoDB" id="310097at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8138669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="538" mass="64907" checksum="1004822250D278EF" modified="2022-10-12" version="1">MEQNLKQMQELLINEIINKQYNEDDFQSFAFERFPDKDELNKWNYDDLNQLVKEYQKLQQQGINNKDKINQQNQDQNIKEVETRPLSKQSRLIPKKPSQQVFLNVITEEQAKLQGFNQQEMEVLNQIKQERCQIQFDKQVVFSEYEKIYACQYQKKCIEENLQLQFKVITKNNKKIIFSNQVFCIETQPFNWKVERTKEDLQTLEKIMSIFFPEYSGCILQYDQKQDFHLHLENALNIYYNKQKTRSLDYFLLFLNDEMAKELHLQPFSYYTQHEKKIRNFQLNQISTSSGQLKIEFNKLNFQGYQKLKKFQQQVYQNYRKFEIKFQESQQIINLEIEKMKVQLQTLKDNNNDIFIQDQFTQKIPGFNFDLFYDHISTAIQISNNSKNFISSTIEQISEQLEKSFKFLEQPLEKCYKNEQELRNIYINDFLPTRKKFLNDKDVQQQLTLYGLNNESISDYQYETISSIIRDVLPLKVHVNYDVLQKQKDQFAYLITQVLFEAQKYLSKLFKDINCICKNNFDQLTQNINISLETKQKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SMU7</accession>
    <name>A0A8S1SMU7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0090280</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SMU7"/>
    <dbReference type="OrthoDB" id="297405at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000445">
        <property type="term" value="C:THO complex part of transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040132">
        <property type="entry name" value="Tex1/THOC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22839:SF0">
        <property type="entry name" value="THO COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22839">
        <property type="entry name" value="THO COMPLEX SUBUNIT 3 THO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="178"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8140786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="370" mass="43605" checksum="B6E56F0999CE1555" modified="2022-10-12" version="1">MEGYNIELVKSKQFQDLDIDAWRMNTLILDLKRQLLVLGCTNNLLFFSITGLKLKQLKPIFIEQNDMINQIKQYDHLLFAITMNSYIYIYDLDNILIKPIKLQGLYEKIQDCSIWSIDICHQYLAVGSNSHIISLWSRKLLNFDKPDHKYWDDREIRVKFNKQDLMRTDIEFPIRYSIQNHKHNIPCVSFSPCCQFLASGSIDGGLRIYLVENGTQLYSIIFSEWVWCISWIQLNDQTKIIFNSEIDQLSQYNIVAGFKDSIKLCQIINQELKVVEDDFYKNSSSLGQRFQRLQYIQDLNLILAFPQHDKQFCLIESYNLDRIIKNQDLTIKLPAENLISADIQQIKDSYEAIIAVLTWQKNIKLYKLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SSN8</accession>
    <name>A0A8S1SSN8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0110022</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SSN8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8142217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="150" mass="17436" checksum="9E345188E9519229" modified="2022-10-12" version="1">MKSRYQGTMNWDFGHQNNMMPTLTLVQSRNAGFGSPGLLLERPQVAQDLCPLLELIKEHTLYDYMEFQKQFASMNRTNKEDQIGIIYNFPSQYHKQMIPNPDYIAISIGKQVDTARKTYIIEKSLRMCKFMVSLMRGISKKESKKLFSAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T399</accession>
    <name>A0A8S1T399_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Deubiquitinating enzyme A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0160128</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8146940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T399"/>
    <dbReference type="OrthoDB" id="415023at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22752">
        <property type="entry name" value="OTU_OTUD5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.80:FF:000018">
        <property type="entry name" value="OTU domain-containing protein 5-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050704">
        <property type="entry name" value="Peptidase_C85-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419">
        <property type="entry name" value="OTU DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419:SF7">
        <property type="entry name" value="OTU DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OTU" evidence="4">
        <location>
            <begin position="215"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="3">
        <location>
            <begin position="540"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="411"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8146940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="583" mass="69361" checksum="9404122482265D3F" modified="2022-10-12" version="1">MEQSDINTGDRNNDNSSSEEIDLELQDDETQVNQECPQEQQVLSHLLEIGEYIDYLDSNTNKWATMKIVDIQNSNIRLQSPYSSNTFWIQFSYEIIRKQWIPGVPLQNTRVEVYNSNKGWLIGIVVQLEKDQALIHHQGYKRIYDQNYLVKDIYPLGQNSNEPGIGKLLKQQPQSILRLQHRFNLYLQLQNLITMRKKYKEMETSLQSILSEQGMLIREVSGDGNCMFRAISDQLYGNEIYHKELRKFAMQYILQEKDYFQDYIINGNVEEYVEYKSKDGVWGDNIELQAFRELYDIPIEIYVCSKEPLKTGLEANPYNKEPIRLSYHGRSHYNSIKLKDKQHSALLGSLEISQYEKQMFEKLQEQKNKQSVNTNNRHGQQLSRAQFENCISNELDIIFNESKRLFQDQQLKNEQTAIKESLQQLEYEEEEEQLMDMVIKESVQDAQFNQQFPIQQQQKQTQQLQQQQQQQQQQQQQQQQQQQQQQQQQQQQQPNLESEILRKIMEQSKQEQEQQFMQQFEDTQFQNALKESEKTIYEDPMNNPIIAQVMDMGFFTLEQVIEAYAIHRDDPDMIINYLYENFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T3R7</accession>
    <name>A0A8S1T3R7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0150208</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T3R7"/>
    <dbReference type="OrthoDB" id="285714at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46809:SF2">
        <property type="entry name" value="GH21273P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46809">
        <property type="entry name" value="STROMAL CELL-DERIVED FACTOR 2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIR" evidence="1">
        <location>
            <begin position="6"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="1">
        <location>
            <begin position="135"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="1">
        <location>
            <begin position="206"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="1">
        <location>
            <begin position="270"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="1">
        <location>
            <begin position="327"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8146166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="388" mass="44712" checksum="CE915F44318CCA96" modified="2022-10-12" version="1">MDKQYDLQLFSGAIIQLKHLSTNDYLFSNRIKLTSGSKETMLGCTKNQYQEGTKFEIHLAYTDFGNMLYQPIKYGDLVYLKQLDQIVTININNKSQTTNQKEAILMEKQNEIMGSQIFVISPSELWQGSILKIKESIINQASIIRLINITSNLSIHSNSNYFKQKDLSDCVEVIGYNERDQNDDWFVNIHDINLINGIIHLLPRFSKPIFHGSLVIIRNAFLGTALNSHSILLKSKNQEVTTNSVIPRISNELWEVVLMKIEGERKHSIENQLLYGEKFSLLHSNTNQFLKYNKGQTNSDKTFQVCCSDQNLFLEEWSIIPLSQQQTQAVFCNDFFLIKNNTCQQYLTSIAEPSYITKQQYKTFLTQLPTNASVWTIEAVLNNKQNYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T4J2</accession>
    <name>A0A8S1T4J2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0150299</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T4J2"/>
    <dbReference type="OrthoDB" id="306763at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050907">
        <property type="entry name" value="SRSF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23147">
        <property type="entry name" value="SERINE/ARGININE RICH SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="11"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="5">
        <location>
            <begin position="89"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="101"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="101"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="128"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="142"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8146347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="160" mass="18335" checksum="5B8C56E60ED0432F" modified="2022-10-12" version="1">MSMSDQSKTENRIFVTGYSTKDKNEDDIKTLFSKYGEISEFSWKGRFCFIAYSKAEEATEAVNEMNQKDLDGNTFVVEIARAKKKDGECYQCGRIGHFARNCRQKRRSSSSSSRRHKRKSRKYKKRSPSSSSSSSSSSSSRDKKKKQKTKKRKQSSSSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T7Q6</accession>
    <name>A0A8S1T7Q6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0160387</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T7Q6"/>
    <dbReference type="OrthoDB" id="295329at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8147426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="165" mass="19460" checksum="D09E44AD977ED2C3" modified="2022-10-12" version="1">MKQLDQRITALEQLIGDGDLVITETIYNQIQQMREICRQQNISSKLYKMAQLIYNYDLNYCYSALPSQSKIQLIMQSEKQLQEASSNIIKIIKYRKFVDFEPIIGLQEKINQILLLQTASKLAFKDDQIVVNQNNTMMLIDDFNRYQQQLEALVLINYELRQQVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T995</accession>
    <name>A0A8S1T995_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0170206</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T995"/>
    <dbReference type="OrthoDB" id="296750at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="189"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="243"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="286"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="369"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="3">
        <location>
            <begin position="472"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="696"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8147916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="859" mass="102399" checksum="F0EE81E6AA1A3C0F" modified="2022-10-12" version="1">MEEILVSQRMGPSALTQQLSSKQESPCNLMLEDDYQLESQRDMIENKSIDMNMSRFESKTEFKKHAQFLTNRALRTIQTQKQSHFLQSFKLQLHVHRFINNLFTNSYILRNTQKQKIQDKLLEKQFISQHRKKDYENKSVIPIFLPSTNSIMLWDIFGFICNLMMLWLTPFLGAFNNYQNQTISVLQQIILIQLIFDFLAQFNRGIFVSAILITNRKKIIKQYLKSNALSDFLRLIIWGDIKYQILFTFCFEIIIVLQIILIYQKILRYLQEYYYQYIYSKGGQNFVLDLVQLIIQIYYFAHIIACVWHYVGENTYYLQNSWILENQLNEETIWNRYNSAFYWATMTMTTVGYGDFSAKNQIEMIVSSFIMFFSSYAFAYTMSSIGIILKNVYDTKQTYKKNLIQMIQYMSKNQVDESTQGRIRNYLRFQQAQEKKENQDEIANIINQLPKNLQQDLNSDIQSRVIKKMKLIINHFSKYTQQQVAKNLELIQFIPGDFIYKQGDQHEDNLYFLSTGDVILMECQTEQNLRSVKNSQYLGYYSFFTGFSPKETAICQSPSELYKISRKKFLDIIRQNQKDFEIFHHIKEKLIFSTNYVLFDHKCNFCNRYIHQEIDCPLIQYKPDLEAILKKENFNQILNLRRQVIRTKRKHHALGQHQPISQQLKLYQQDNQFCEDTDSDQEGKFGNQKSFFSEQQRSSSQLLQSEDDGHFENTPPRQNQKRATQHYGPLKLQKMSSFSKQKLGQVEQKTSQRRMLIQNEEQIAIKNYCMIEEVQLLLQKQFKPSFNLDKVCNTFRSYMPQYTIESQIKEVQKVQKKKQRRFWKQNEKLGKYTFSNNVKVLTLKILNQGKQINKNVNQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TF49</accession>
    <name>A0A8S1TF49_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL22</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L17</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0200143</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TF49"/>
    <dbReference type="OrthoDB" id="304509at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030684">
        <property type="term" value="C:preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00336">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.470.10:FF:000012">
        <property type="entry name" value="60S ribosomal protein L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_uL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005721">
        <property type="entry name" value="Ribosomal_uL22_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01038">
        <property type="entry name" value="uL22_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593:SF10">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8150204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="184" mass="20710" checksum="4C0B168BDCF34445" modified="2022-10-12" version="1">MGKQYAREPAVAKKSAKAKASDLRTHFKNTYEVARALKGQTLAQALKYLQDVLQHKRCVPFTRFNGGVGRTAQAKEFGRSQGRWPEKSVRIVLSLLQNLAANAQVKNLSNEKLIINHVQVNRAQKGRRRTYRAHGRINPFLSSNAHVEIWASERDENVKKEANNKVVARQSRKQAAKSRLAIGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TLP2</accession>
    <name>A0A8S1TLP2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0260232</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8154941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TLP2"/>
    <dbReference type="OrthoDB" id="421575at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046621">
        <property type="term" value="P:negative regulation of organ growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033276">
        <property type="entry name" value="BB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46400:SF5">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46400">
        <property type="entry name" value="RING/U-BOX SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="180"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="31"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8154941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="229" mass="27232" checksum="8D83911863FB5DFB" modified="2022-10-12" version="1">MDNPIKLSQLNSSSEHKEIVDKFFKNPIQQIQKLNQKSSSKSDIINELRQENYDLKNIVNDLKQRIQELEQQLQNQQNQQIEEENQEQVSVEKQQNEIQEEMTEEEKSIHLAFMLQQQEEMEFQNRLTQMQNNVDLDQMSYEQLQELQEKMGFVSRGLLQNQIQSLLKQCKIKQQINDCCTICLEDSGNPVEIELECCHVFHQECISEWLSREKHCPVCKRDINLSKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TMN7</accession>
    <name>A0A8S1TMN7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0240094</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="317295at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026306">
        <property type="entry name" value="RSBN1/Dpy-2/CEP530"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13354:SF11">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13354">
        <property type="entry name" value="ROUND SPERMATID BASIC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035816726" evidence="2">
        <location>
            <begin position="16"/>
            <end position="1417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="631"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1248"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="631"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="643"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1248"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1267"/>
            <end position="1280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1281"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1301"/>
            <end position="1330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8153223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1417" mass="160073" checksum="77CDE8B7DD4E3571" modified="2022-10-12" version="1">MRMHALFVLLMYAAALNFEASLMSHTLAEVDALDTSQLNCQTPRSHFDELEQTLTQWQNVLENKERIPSHIETLTQMKHLIKHKKYSQLVDKIKDLDLISPPVLSELQNKLQGLQSHDKHEREECTETLTKLCAYLIKQMNNVHQQCQQSPVTIIKVKGKIDDLKIIQSGCQQACQPTCPDEPVPEVPSEVASEEFTEEPVEEGVPPTTPEDEPTPDEPTPDEPAPEDKPTPDAPEVPQEEEGPTDEPPVNPSSEESPEETTVPEDGTEEWTIPGEEPEEPKSVPTIPDSPEEDQEETPLPPVSPDPQEFPEEYREEFIFEETPLPPVSPDPFVFPEEDEEQFIFEETLPPVSPDPLVFPEEDEEQFIYEETLPPVSPDPQVFSEENEEEFIFEETPLPPVSPDPLVFPEEQEEQFIFEETPVDVESPIVCHSSESTEKIELTATQVEPGFTSTLTITNEYGFGLYFQKLQKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVNPSSEESPEETTVPEDGTEEWTIPGEEPEEPKSVPTIPDSPEEDQEETPLPPVSPDPQEFPEEYREEFIFEETPLPPVSPDPFVFPEEDEEQFIFEETLPPVSPDPLVFPEEDEEQFIYEETLPPVSPDPQVFSEENEEEFIFEETPLPPVSPDPLVFPEEQEEQFIFEETPVDVESPIVCHSSESTEKIELTATQVEPGFTSTLTITNEYGFGLYFQKLQKYPAFADGEEFHLASLQDNGNTVLGVFVTKTGVKCVTADGSTQQSTSVQNDDFEGDWSFVFISHGKGVTGCGVKFFEEQPALRQTPASHPNFSTLEFIIGGEQDSHPSFQGQVYGHFAVSAPNLHFQDEKTYKQIVDSCNNPPEEVCELDTHELGDFEFSGFDDNSEERYDFSTWDSGYSVSGWFKWESLQDQDVWHTVFRLSENTNADLDDIQLGDRDLALYLGNELVGNRYQVTTYTYSGSGNPNLWDSVEYGSTLGQWHYIYFGYSRRTRYANYAIYLSESEMPSNQIHDVRHYLVPQRRLYIGNDGLYPAFNGKFYNWRVNSCIGDVAIDVVNPPKDIIFGYNPQPEVPQIVPEVFPEEETFWPEEEEYVPTNPEEVPFDIPSEEESIWPEEEEEFVPPPPTLIPEEESIWPEEEEEFVPPPPPEVPPVIPDEVKPEPLPEPQPQPEPEPAPAPSPKPAPGPAPPSEPQVPAVKECEATIDVTKENAADILCAISEYLAQLASGHVPELGINPSRVCFCLQYDDSDSNESFMQVEAILKENDAYTINQLVGQRRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TPI2</accession>
    <name>A0A8S1TPI2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SMP-LTD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0240247</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TPI2"/>
    <dbReference type="OrthoDB" id="300899at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21675">
        <property type="entry name" value="SMP_TEX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13466:SF0">
        <property type="entry name" value="SMP-LTD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13466">
        <property type="entry name" value="TEX2 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SMP-LTD" evidence="10">
        <location>
            <begin position="663"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="258"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="437"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="614"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8153487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="851" mass="100973" checksum="51899338E0274C41" modified="2022-10-12" version="1">MDYLKQQHKNYEVSITSLKQHVDEYLHPIKLKRKHYINKFNSELQIENPQPKQEDKQSIQQISVQKLQRKKYLKKLDIYLEFFEGIKESEQQFGQQLNDLDSKLISENEITIIDKYTEDGNDIIQQLTGIQLSSKLFLYLPFWKDYKKVLKQISQCHTEVAQDMGKVSFQLYQLKKGFLKDVEDIRKQRKKLKHLTVEIYQHYQKILNEFIQNEKDLQIFTQAHSEMLKKKDDPTQIIKSEMKVKLQMQQQDNIRLKLTDARDRIKENQIEINEFNQKMQQFQLNYEEQRVIFVKQGFQAFLITSENYASQFQTYISTLIVIIDEDYYQIYPSRQHRSPQSPQKQFLENQQQSQILELDINEQIKEIQRQTTYQLQQWQIVQKYNEELEYFFRDTIQILNQKSVDKDFSLVKQQVEQEKQEVSDVFNILYSSLISVIQIIKDSNKQLVDQIERKRQDIAKCRLDMSIGKELEICALRELKKLQSLSTDEKALEQYQNDQVDKSKTKKAAQHFLNEMKQNIQDVKSLNFGKFYATIGQTVSKVQKMSNLKEDDLREQQIKLMEDKKALIQLQGFTLKKMEDFLNKHSKLVAKMKKTLIEDLGRFAISIFTHLRMIVDETTENIQIMQQRENLENQIEQQVIQNEKEDPTYQNEEIVEDLHVSTTSLLLQKLLKVLVNDWKESEYFKGKIKKMLHKAFNKKRVNMLSEITVTDLKIAGDPPSLSEIKALRQDVNEFLCDLDLSFRGKIQIVLNTNIIINWPKEYQVPVEIKITISTFTSRIRLCFVPTYLGKSWLSMIGQPVINLDIEPTFLQKYNIAKIQQIADLIREFLIGKVKLMTYPNKLSIKIPLSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TS63</accession>
    <name>A0A8S1TS63_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0260202</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8154883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TS63"/>
    <dbReference type="OrthoDB" id="307256at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="172"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8154883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="227" mass="27096" checksum="33C1926AE2C753F6" modified="2022-10-12" version="1">MHSHQKQLIDINSLIETIENQFRKNEEMHQTISQQIHCDIERIDAQLFIQEMRISLKQPKASIETLLKKCQSMIDKLLDEEFNLDRLMSKLRSKKRRNNKASTGNVLQQIDENTLKEINTFSKCQDVVKKDEKQFTEKLNIEKTNRHTEIDPFEKLININMQIEQCLELLSNQNNLDNESDMEVKINNLEEQIYKLTENRVYNDENVEVYIVQKNNKKRMPNLQINY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TV52</accession>
    <name>A0A8S1TV52_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0270088</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TV52"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="368"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="497"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="628"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="507"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8155432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="852" mass="103715" checksum="01149ADB484CBBDA" modified="2022-10-12" version="1">MFDYDQKEFQETIKMKCKEREDQIINQYNQVLFRYQAECQEYIKNLKQQYEKDQENFNQQLIKKLIELKLIVDNEKKNLQLKEDFEKEQQKNQLSEKFIQENNKLKMELNDLKNNFNKKVSIKVQEELNKNKQYKDEIEALTQKFENTFNQLRQKILKQQNINSEMNEALQNLKNEQNNQNNSNEEIKKSKAQIQELYLQGQEDIKQLKQKYKTDKSDLEQKNKKLEEQNQRLQEKHKYFVNQSIQQQKKLNETEDNLAKAKSNFQDQEIQQKKKLKEIEDSLAKAKSDFQDKEQELLKQKRIFEDEKSKLAEDLQKQNIENNKKIEEEIRKTEENYEKQLQSQTKQFEKKRSELEINLKKAEEEGRIAKEKMKEAQELESKAKENLEKKQEQKEKLEKAKQELEKQVLEHTKDAQEQKNQTKIIKEENQQQLEKQQKVISELEINLKKAEEEERIAIYKMKEAQELESKAKENLEKKQEQLQKQKKKFQIAKQKQEENAYQIQQQRDNENKKIQEQLEQKNKEDQEQYQEKIQNLTEQYQGKEKELQKVQENLDIQAKEFKQQKIEAQKIFEEKEKQIQQMQQEISEQQQLVGQAQEEVKKVKIQSEKYQKELQLLQQRFDKEISEQEQKIKEAEQQMKKAKESQDKYKEKLKIKEKEKSDIEKQLEISKQEEEKAIEKTLSVQQEMEKYKEIQEKYQERLKIQNEEFQKEKSDLEKQLEISKQEEKKAQEEYQKYKEQLEIQKEQKQEEILKLEEKHLYEISNEDIENQLIYEEQEKKDKEQQLKQRKNSDFIYINDNIKTQLEDIKKKVMMTNQQLVNSNEELILTLKINQLLPSGNSNFLKEYQQKYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TXQ2</accession>
    <name>A0A8S1TXQ2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0290327</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8157078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TXQ2"/>
    <dbReference type="OrthoDB" id="10315986at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="111"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8157078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="166" mass="19743" checksum="061C613B48A2F30B" modified="2022-10-12" version="1">MFGKNIYQDDVPNFPEISEDYFEIKDEKTINELMDLYNSSKILIPVNGSVSNEFREKQLGLLSLFDLLDNQLKSNQCKPDIYEHNEIDCSINTPYQNFDYYNLSNIDYDQNEAQKEAAKFLSNNKNELQEKFEKMQIETKHNSKQIQHEKLTKKMELLSFQDQEYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U1E2</accession>
    <name>A0A8S1U1E2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0310294</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U1E2"/>
    <dbReference type="OrthoDB" id="313342at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="220"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8158348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="280" mass="33469" checksum="28B8187CB2D68251" modified="2022-10-12" version="1">MSKQLQNEKQQNVIQASGLQEAYEYLLRQLCKKGLPTGNVYEFSAQTILKYEHKLKNQNIQNKAQQAAKEAHEKFLKKMQDKEQKNLEEVSKYELMFREEQKRKELQHQSISQEPVQRQSVDKQSLDLKNQQIDKVDQSPPKGQKKQQDIHSSPPKQEIKVQKQETEQIAQQQQQNQQQQQNNKKGAVQNQKKDSKKQIQNDQSILKELKLEEQKEQQKQQEQQKQQEQQQQQQQQQQQQQQQQQQQQQQQQQQNKVNDINKEQPKEQQKQDNKKTSKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U4Q9</accession>
    <name>A0A8S1U4Q9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0320307</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8159083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U4Q9"/>
    <dbReference type="OrthoDB" id="300710at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8159083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="131" mass="15120" checksum="C7C7104ABA74FC5B" modified="2022-10-12" version="1">MKRNRVMSTDGVLAVITDQLLTENKPRFRKLTKKQMMQKKHTNDLDSSFEIIEKNTPQLKNKKIKITKQALDHFINKTFVVISDDEAEILINHSNSIKNETTSRSSSILSFSSSNLEQWEQAEKKVKIGEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U582</accession>
    <name>A0A8S1U582_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0320163</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8158797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U582"/>
    <dbReference type="OrthoDB" id="292705at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8158797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="636" mass="76568" checksum="AD5C3D7C0CBC054A" modified="2022-10-12" version="1">MYSQLKKSKSHSKTQMLYKLLEQTELYSLTKDIMDKDLKEFWELCQTAKNEFYYDFQENPIHKVQLFLFKIDEILNNPFNLLMKQKIKSLLIELFAQGTQVPQIFGFIQFKEDKVLLQCSLNIEQQLKTMIKQDQKCQQIDYYYKELLMNSEELLIQITLNYLYEAMMEENQLELKNNQQLDSIYNITNQINKQKFSKNGQKIIELIQNIKKRVNQFQDQITIMKPIGHNNQNQQNQQNEKQTENMVSAQENGKYLIFKILLLLLLKQDIALPDIIKLFKISYVCKQQHMEDRKGFIKVYISQQLIFQISLMVKIKQDAELLKINSSLSYYDESSIRAVKQAIKQHFPEYEYIKFNQVIKSSLELLLKKFDENYEQICATMEDSNVQIVLKKIYKICGKYFQKIEAGIITFQNAQQKFLKNNMLEIGYLLYQIQIKIDKKHNNQSIITYQKQSIEEQNNYQQINYQRSIYEKQLIQYGIINPIFFKFIQNLIQNQIPFPYMFQFLKMGCHRLLIHYRIESTRKLTLKKKFIEFVKQNYDLTMTDDRYINVELLKTIDTEKYVRKEGLAWPLLLYEQCKLIYENRERFKKKLQQLGSNKDFQEIIQQYVGQFESNSIKFKLQQYLLELNNYIKQLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U694</accession>
    <name>A0A8S1U694_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0340196</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the ATP:guanido phosphotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8160158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U694"/>
    <dbReference type="OrthoDB" id="430219at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004111">
        <property type="term" value="F:creatine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046314">
        <property type="term" value="P:phosphocreatine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000037">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000749">
        <property type="entry name" value="ATP-guanido_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022414">
        <property type="entry name" value="ATP-guanido_PTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022413">
        <property type="entry name" value="ATP-guanido_PTrfase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11547:SF38">
        <property type="entry name" value="ARGININE KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11547">
        <property type="entry name" value="ARGININE OR CREATINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00217">
        <property type="entry name" value="ATP-gua_Ptrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02807">
        <property type="entry name" value="ATP-gua_PtransN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51510">
        <property type="entry name" value="PHOSPHAGEN_KINASE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51509">
        <property type="entry name" value="PHOSPHAGEN_KINASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphagen kinase N-terminal" evidence="6">
        <location>
            <begin position="68"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphagen kinase C-terminal" evidence="7">
        <location>
            <begin position="174"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="463"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="472"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="177"/>
            <end position="181"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="340"/>
            <end position="344"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="371"/>
            <end position="376"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8160158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="963" mass="113189" checksum="3B101F04140E77B0" modified="2022-10-12" version="1">MAQQEIQRKAKAYLDKHQIEEILGDLVTTLSYHQMKNPIIYIIKYLCTVHCTPKELQEAGIQIDNREKVEQPILCLPFPNLSQHQGSIKKLTKEQFYACKNKITDQGNNFRSICKLIQDNPKSKPGLFAVDPSSYLIYSDLFDSIVKEKGYLGTPIKDMSYTLTSSILEDVKPFLEESIFVLRRNVPKQRFGSTLQNRQALTKELLQVLSKQSIINYSLHNDLSAMAQEDVRKLQINDKLFKKKDPNQAIINQIFKGLRHPDWPSDRMVLQSNDKQNIVWINREDHLKFKFLNLEKSSIIDALDTCCKMNQYLDSKDLVSFDDKFGYHTVKPQFSGLGLTFTLKFKLDPQSINKIKSNNNNLSSKIQNKVFNVQTKEKDKYFTIKSERCTGLTMKQYVEQLCQILSILFEGVIKKPETQTIEKQFKNELQIQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQSMAKLQDENNTMSPSKPASSPSHVAIDILQNQPEEQEYATLHKKRLETFSKKQKEINKTRWNKICELKELIGLLKDDTFYFQNINKKFMIVRRNLKIRRKNVEEIIGLKNQQVSDQILKECNLNGNCDKGIFQYKYKGYEYYVAIELDDDLTFIFPIIKISISQQLQKIQRIVDQFLPKDQQYAWDNQYGYLTNNNNYLGSGISITCDFPQFIQNEIPGILKELIQDDSIQNSNNQFLTLTSLDEYNLQIYRNAVNEILLKQKQHNQLIKKAADNQICESDCYINDQLWAPSKKCFEDFEALYTQYIWNQAYFNGKHVTPFKNYDKIKPDSYKLNIPQNPTIINIKCTFYRNFESQKYKHEMSIQDKIKMKTKYNEFMKNNGWKFGKTHHLEISFDSKPEEFIQNFCNHWNSLIEQGQEFQKNGNIGFYGPNLNQSLLEGFQLEIKTNKPINPELIKSFNYKPYYQKNGMINQEDAIITIISNRPCVLEFELNLIQELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U6Z5</accession>
    <name>A0A8S1U6Z5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0330188</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U6Z5"/>
    <dbReference type="OrthoDB" id="428895at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555:SF170">
        <property type="entry name" value="FI18122P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="2">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="169"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8159517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="387" mass="46284" checksum="6F455D1F5E1E33CF" modified="2022-10-12" version="1">MNQDDFEISVEVSDPKVQSGLNKYTTYSVRGMDKNGQFDVIRRFSDFRLIRQFLITKWPGCYIPPLPPRKAIGNMDQKFIDDRMNSLQEWMRIMAQTKYFWYSEEFQLFIKSNGDIEKALSQVPKLTYDEIINKYQDTFTDLSGREINRELIQKITDFHQFLKRVNPMVENFKQIAKNIAEAKQNFQEQMANFLQNQLGAYEQQVLLEFSESQDKLVIQNIKNDEYPSQLLNNYINLLQQNEFQSLYQEIKKESKQVRAFLETMIQREKYEQQKVFYEQKQKELQIQLQEVLAGKSSIKNLFSTTKKEDQIAKLQVQIDQVCKDIENMQFICDILTVLLGYIEIDKFKLEKQQNYYRLAKSLIQYEIQLNQASEEFWERVQQNYNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U970</accession>
    <name>A0A8S1U970_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0360085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U970"/>
    <dbReference type="OrthoDB" id="304561at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="44"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8161268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="73" mass="8712" checksum="9563A0FF003DF7B3" modified="2022-10-12" version="1">MNSKNYSQTYSQCYSEETEEKVQSLSNMEIRWLNLLKRVSGDDLEMLRIQLEKQKQSLKDIEKGIEQQIIEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UD17</accession>
    <name>A0A8S1UD17_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0370066</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8161863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UD17"/>
    <dbReference type="OrthoDB" id="317647at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8161863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="224" mass="26543" checksum="1AB2D105E216D32F" modified="2022-10-12" version="1">MISEYNYMYKLNDQLQGWDIEQSIDSDEGDKKEINQFPSITKTQPSDRLCLKKLFLKSPQLSPLRANPPNHQTTPLRESIQQKFVFANQFINKKQEINKVCEEQSHKVMNVNAKVQQPINIANLRIKLNGIPKQPQHIKAYTQNQLQAILHLKRRNLYLRSVLKKVCHESDERYSNHQKQEIFLGYRYVDEDMQKLQSQNNSMNLQLQSRKNSNIRHIKMKQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UGW4</accession>
    <name>A0A8S1UGW4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0410014</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UGW4"/>
    <dbReference type="OrthoDB" id="288496at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8164401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="305" mass="35444" checksum="E78E7CA680D8DAC2" modified="2022-10-12" version="1">MKLKLNAYCHFYQALINTDQLYCYKVCVYVANGYIQQQMEIIETYNNSKQLIGGSYNQNELVIAQWGGNILFKRKNEEDVKINTFCVGLIKITGDKYLCFTSKNHLLQINEDRKIEQIDQQDSPYFVCDFYLKTCLFMHNTIQQQRFLCLWNMLTQQKSKYEHISSITSVMAADDGIWLGDKDGYLNFLTYKLKLQKRVRIFSNEISKIQIMNKKIYCVSQRELKLFFQDQIVSISTFDNEISCIGAFKNKLILGTTNGQLMIQENQKIFAQHQVHANSITSISVNGNQIVTTSSDRTAKIIQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UHE0</accession>
    <name>A0A8S1UHE0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0370193</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8162086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UHE0"/>
    <dbReference type="OrthoDB" id="292734at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8162086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="94" mass="10664" checksum="589D26041B7E9B14" modified="2022-10-12" version="1">MRGFLSLAKTIVYSSSIIKPTQQIKMQFRLANQKLASQFKLCSTIQILRMINLQGVCSNSQLEEADEQDLESTISKLLSVTLKSNTKKHQFITK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UKH8</accession>
    <name>A0A8S1UKH8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0390074</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UKH8"/>
    <dbReference type="OrthoDB" id="285620at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8163146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="152" mass="17782" checksum="A50163D34AA9C59E" modified="2022-10-12" version="1">MEQIESLEGKLFYKIYMLCKGNQINEFQKGKLKDLVIQRDERIGMAMTQYLEDRDEFRLLENFHNCSCEEYSPKSYNNLSDDVSNGSSSKYKGKRPAKMQLPLKTFMINLRKNTDITTSSKGFYTSTARSGGNRNYANFNERFQQSNWSECE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UU61</accession>
    <name>A0A8S1UU61_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Carboxypeptidase</fullName>
            <ecNumber evidence="1">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0480185</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000048">
        <property type="protein sequence ID" value="CAD8168095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UU61"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000253">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF201">
        <property type="entry name" value="CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036518741" description="Carboxypeptidase" evidence="1">
        <location>
            <begin position="17"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8168095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="475" mass="54067" checksum="755D847E3206BB27" modified="2022-10-12" version="1">MNNFVIFLSILSICLCLQVQVQVDEADLVDKNELNRLFKINYDGQVYSGYLKANNEGTAQFHYMFYPAPVDALKKPVILWLNGGPGCSSLQGAFNENGPFVFKAGTAEFEMNQYSWTNFANMLYIESPITVGFSYGPQGEQSDESTAKYNINALVDFFSRFTEYKKLPFFISGESYAGIYIPTLANEIIDYNVGKPTDSLINLQGLAIGNGCTDPTECTEQADPFQIHVYKFYGRHNFISEELYEKILAVQNECYGVKDGKCKELADKVEVEVSGTKAAHIKFNPYNIYGYCFTYTPEGSKMSQKFSGMRSLNEESDIPPCADVQGLYHYLKSAEIRTLLKIRQESAEWSICSRTLGNYQVNPKGSYYLYQKILKNQIRILKFSGDVDAVVPLTGTMFWIDKLQKELYLTTLKPWRPWFIHAQREVDPDQNAGYVMDLDGLTLLTIRNAGHMVPLDKREESEIFMQKFIKGELFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UVJ9</accession>
    <name>A0A8S1UVJ9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0440182</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UVJ9"/>
    <dbReference type="OrthoDB" id="299647at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8166506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="296" mass="35448" checksum="5320F2925C94F0CD" modified="2022-10-12" version="1">MKSLKTNRTSNNQCIIGEVGYYKQCSRFLPLERADEFSTQDAIIYDRLRQAGMKRNKALQFCKTQHCKIDSTQTKHGPPGFKIFNTKIDKFKQQNGWQMIGRLNYKQFMREELSDDTQWQIQDQNKEEQQKMEQLKQFYTDLDVKYSFNEMDQQETLSKLRSLSTEQQYRSRVPQYQLKDGKNQMLYYEMAEYILQLKKPPNTLIQKLNVFDNTIPLVRQEYFDIKPKYIEKATPIYNKLNCEKRSKLLKELTRAYTGNITITEPTEEIKKVLNNNNHQMKQFYKVQMISTKKVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UVT7</accession>
    <name>A0A8S1UVT7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T0440227</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UVT7"/>
    <dbReference type="OrthoDB" id="302094at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089701">
        <property type="term" value="C:U2AF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12287">
        <property type="entry name" value="RRM_U2AF35_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000122">
        <property type="entry name" value="Splicing factor U2AF small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009145">
        <property type="entry name" value="U2AF_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12620">
        <property type="entry name" value="U2 SNRNP AUXILIARY FACTOR, SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="13">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="13">
        <location>
            <begin position="149"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="11">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="11">
        <location>
            <begin position="149"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="195"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="195"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="209"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="229"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8166596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="302" mass="34689" checksum="37E63BF5449FAEA6" modified="2022-10-12" version="1">MAGGLERVYGTEEDKVNCSFYIKIGACRYENKCQRIHSIPPISQTILFKHMYQNSPMEVAIASGNAVSQAGIEEALEKFENFYEDVFLKLAEFGEIEDLIVCENIGDHLVGNVYVKYTSELYAESCFNALQNLSYENRPLSMEYSPVLDFSSAKCKQYIDGTCQRGGACNYLHLKKISTKFKKSLFNQMYEEHPEYREKKEKEVNEKSPKKHKKKEKKSKKKKTSSSRESSRRNSIERQKMINDWNETGIQNVSQAQKMMYGNNMPAPVYTPKVSMRTSPELLQLQLQLAALKEQLAAIKMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UXZ4</accession>
    <name>A0A8S1UXZ4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Clathrin light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0490082</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasmic vesicle membrane</location>
            <topology evidence="1 6">Peripheral membrane protein</topology>
            <orientation evidence="1 6">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <location evidence="6">Coated pit</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="6">Cytoplasmic face of coated pits and vesicles.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the clathrin light chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UXZ4"/>
    <dbReference type="OrthoDB" id="302617at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030132">
        <property type="term" value="C:clathrin coat of coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030130">
        <property type="term" value="C:clathrin coat of trans-Golgi network vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000996">
        <property type="entry name" value="Clathrin_L-chain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01086">
        <property type="entry name" value="Clathrin_lg_ch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0168">Coated pit</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 6" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8168419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="165" mass="19297" checksum="2CA3A097399B818B" modified="2022-10-12" version="1">MDEFFDDPAGTIETYIVPEVAAQPLMFNDAARQPFEMSIAPNAGVTVLVSAEQQQRREKLREVEEERIKLIRERDQEERIQKQQKKEKAQQYLLQFQTQLKSDIQARKDANKQAQELRSLNKSQYKNSWDQIAQNVALKDGEYPGEKDVTKMRQAILNKRKDLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V587</accession>
    <name>A0A8S1V587_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Diphthine--ammonia ligase</fullName>
            <ecNumber evidence="2">6.3.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Diphthamide synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Diphthamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0550088</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>diphthine-[translation elongation factor 2] + NH4(+) + ATP = diphthamide-[translation elongation factor 2] + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19753"/>
            <dbReference type="Rhea" id="RHEA-COMP:10172"/>
            <dbReference type="Rhea" id="RHEA-COMP:10174"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16692"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:82696"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.1.14"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.14" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V587"/>
    <dbReference type="OrthoDB" id="686384at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017178">
        <property type="term" value="F:diphthine-ammonia ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01994">
        <property type="entry name" value="AANH_PF0828-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000145">
        <property type="entry name" value="ATP-binding domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1490.10:FF:000001">
        <property type="entry name" value="Diphthine--ammonia ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002761">
        <property type="entry name" value="Diphthami_syn_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030662">
        <property type="entry name" value="DPH6/MJ0570"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00290">
        <property type="entry name" value="MJ0570_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12196:SF2">
        <property type="entry name" value="DIPHTHINE--AMMONIA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12196">
        <property type="entry name" value="DOMAIN OF UNKNOWN FUNCTION 71 DUF71 -CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01902">
        <property type="entry name" value="Diphthami_syn_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Diphthamide synthase" evidence="10">
        <location>
            <begin position="1"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8171563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="308" mass="35217" checksum="371D4A4DEBFF0175" modified="2022-10-12" version="1">MKFLALISGGKDSIYNIIRCVQEGHELILLVNLYPKQIGIESDSFMYQSVGTNAIQAIAQAMDKPLMTREISGKPKITNLDYQSKDEDRIGDEVEDLYLILKEAILTYPDIKGVSSGAIASTYQKLRVEDCCNRLGLTSLAYLWNQDQFSLLDQMLQNNMNIILIKVAAMGLTQKHLGKTIQELYDYFKEINKKFGFHPCGEGGEFESFVLDCPLYKKRIHIKAQLEVQIQEQKDTIEDAQSWIEFHTLELENSEERLAGQQAWYAVQSEIYETQTAERAAQNEIVDRLQEHISEKLSTTAQFISKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V9X9</accession>
    <name>A0A8S1V9X9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tubulin-tyrosine ligase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0600186</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000060">
        <property type="protein sequence ID" value="CAD8174020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V9X9"/>
    <dbReference type="OrthoDB" id="202825at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_125, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069">
        <property type="entry name" value="TUBULIN TYROSINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8174020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="507" mass="59609" checksum="8269AB78F3FE1065" modified="2022-10-12" version="1">MFLQAIKVKDCRLEPIQLKYSPQVPRQHTPEGLSKIGMFNSTLFQGKKSVEKKKQSTQGDQRRPQSSFQRITKPAFFKNVFTVQYIFFCENGNNGNLVRRILQGRGWFGEQQTSYNVNFIWRQSNKGFNYSTLTSKNVCINHLEHHNEISNKNKLHANLKSYCQRTNKNMDDFVPTTFIINLDSTTLQNDIKKFVDYFVEIKKDKLLKNVWLLKPPDLNRGRGIQLFSDLKVLVNQIDEFCKTRNVNKQIMSTSKAAKTLIITYPESNEKTGQAQIEFTLDQTPNQDRILVLQKYLETPLLYNGRKFDFRIWVLIDHTCKYYFFKEGYLRLASEHFDINNLKSLFIHLTNNAVQKNHPHYCKYELGNQLSFQDLQQYLTQQKNTKITSVGIVTKMKELIHQTIFCASSKLRNNRKDYQFEIFGYDFMIDNNGNLWLIEINTNPCIEESSPLLQKLIPRMLNDAFRLTIDKIFSPFKTSQENFLNNLHEFSIPGYSDKDNLWDYMGQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VFN6</accession>
    <name>A0A8S1VFN6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0630047</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VFN6"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8175421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="43" mass="5216" checksum="175AEDB26AA8132B" modified="2022-10-12" version="1">MERLLQNVENNNNLLIQIRSLKDQDSIYTLQRKGNCKFQEFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VGC9</accession>
    <name>A0A8S1VGC9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tyrosine-protein phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0630088</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VGC9"/>
    <dbReference type="OrthoDB" id="10252009at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46653">
        <property type="entry name" value="SPECIFICITY PROTEIN PHOSPHATASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46653:SF1">
        <property type="entry name" value="SPECIFICITY PROTEIN PHOSPHATASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="2">
        <location>
            <begin position="4"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="3">
        <location>
            <begin position="69"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="303"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8175503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="399" mass="45901" checksum="DB4512E821ED1630" modified="2022-10-12" version="1">MIIGAIKIKDGLFIGDEFASKDLEFVIQNKVSHIINCAAKQLPCVFENYGVSYLKFNWQENEQQVLFQNDIVNEIYHFIEQAHANGESVLVHSVRGQSRSCCALAAYFMRKYKWKLYKTLEFLNTRRPDLEIRASFYHQLTQLESKLSKKGEGGLSSSWQPHEELDLPLEEDEKLLRNTFQNSKSSAPDSCWLDKRLFNQYLNENRPRTITWADETLVKKSQKKMKQNTAKPTVKLNNQNNINGNVYHVTVNNYVTLKEEQEKCNTSLNSRESAQMSLIKPISGTIKRQQNDSLKKHQELKQKLQGKSQSVKQQKKDPSERPRSAYAQPEIQPMPNLPKPTTGLAYRQYSPLVKANAIPKANQLIATSKNKGWRYPSPGQIKNDESIIQSIINGKPVWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VGX2</accession>
    <name>A0A8S1VGX2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0640238</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8176237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VGX2"/>
    <dbReference type="OrthoDB" id="301196at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8176237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="319" mass="37566" checksum="19ABFA238C5E5A1B" modified="2022-10-12" version="1">MIKRNTQLKKQIASSRNQGVNNKECLKPQVFQFPQLMNKTTTFQEAIRFQGQIQQPFTPDVMIRQSINKPRMIEQPKFKNNYGVQRFSAKVVSKQQNVKEPVIIKITTNKNHQVIQQQEEQSEYQLSELGTFDDLIQQIQEKVLTKNSNIQQLVQDICSIETIDLRGLQCIPQLIKHVVISSVQQQLQIPQILSILFTLSEEIMTRDDLDESLLEIIKVYLQKGYDTNLQMIIVFIKRCGQMIYYIRTNKQKVLSELIIKLLLKFNLSDSFQESQLRLAFNIILRHKQLRLYIKDNQDIYKLKQPSLIDQLQRFKIKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VMB5</accession>
    <name>A0A8S1VMB5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0670150</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000067">
        <property type="protein sequence ID" value="CAD8177355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VMB5"/>
    <dbReference type="OrthoDB" id="288626at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8177355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="150" mass="17791" checksum="CE6B7D80AEB4761D" modified="2022-10-12" version="1">MLNSKKQIKLKLQREQKPFPHLHVIHIDENLQQLCKLLDNQQTLKTDIQKQTKKVLKNSKTIITTNKQSSIIKFIIQNKTKIFSQKENYQQSPIFKSLIIKQSQPLTKPFIGSFREKKIFSVQYPRRLSTIQNKINNSYQDIQIGPWEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VQC3</accession>
    <name>A0A8S1VQC3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0710145</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000071">
        <property type="protein sequence ID" value="CAD8179237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VQC3"/>
    <dbReference type="OrthoDB" id="307950at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="8"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8179237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="346" mass="39654" checksum="545AD569C1EC870D" modified="2022-10-12" version="1">MYLLNSEYKISQSLGSGSNHIVFKAQHILTQSIYALKIEKKPGIGQIENEIQILQELFGIEGIPKLIQFGKTKDLKNFLIIPILYCSLQDLRKSNQLSLSAILAIGLSVLRTIKSFHKRGILHLDIKPENIMISSSEVFNSIEQILTPGNVQLIDFGLSQNLSNSQFLKHTFIGSLRFASRQSHKIEELGYKDDIESLLYVLAYLRNKNLPWQLPQQSANQLMDIKKIGAIKDQTYNTTLLSQSFPPQFYQFQLYIDTLTHSSLPDYDYIEELFKQMLQFNEIQISPRFQESITYIQEQKDLGNSENVKMNESISKLGADTRVVIISDLIKAYTTIKIKSIKDIHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VTR2</accession>
    <name>A0A8S1VTR2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0720135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000072">
        <property type="protein sequence ID" value="CAD8179623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="302862at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1692"/>
            <end position="1712"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8179623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1748" mass="204802" checksum="1ABEF4F1639B3B6B" modified="2022-10-12" version="1">MIFIVFCLTIQVYGYLNLELGQTYNDAIIPWQGDKNYKYYEIDIQQVIKDRDLIIVVKQQSTIGNPDIYISSLDQKPDKIHSEIQCNSQGMDICVIQQPEKKIYYLAVYCEEYCRYNLKVVYQEELMMTNYDDLEFKFNNTSWTEIIRINITQLQKQQIQQDYNLEVSVQVKNVEILQESFLSFMNLGLEKPSLQKYEYKGQDTFLGIQKFKIKKIMTNQIYTLLVEAQQGAIVQIKTRTYGSTRFMNVGEQIEDVISENELGFYVLNVTTDQELFYLGQLILNIQLISYKGYTEMYVNLDENPASLEDYQWKLRDGVTDDLIISNEDLNRFNSKGYYIYIAILAKESYATFELKTQMLNPNFMVMELNKPAISKMSKFKFHQYKFNIKSNKQQSVSVSLRNIKGDADIILKLCKEFWTCKYNEEEQIQFKSKDFQNNNFINQYYYSLNPGNDIIIFDYLPANCLEHENFHICFYAILIIPGQQNDEDELTYSILITTKQDSIILKENTPIKQFVFHEFYNYYKFTVNNDDHIQRLFIQLTPIQGQPRIFSSRTEIYPTKEQNDNQGVQNLIIYGAKGIQSSINGTIYIGVYGETASQYIITAIVFRKQDDWGTIGKYAHQYIQLIEGYPQEIITSHSTDVQLFKIDLSGYSNNNQVKVFLRINSGEFIMYGFDHPEIDLKQAIKTGSNSLILTDSIEKYPQYLFVRVQTNQSTTYSLYSYSIHYRISSQPIELVLGDNYQGFIEKSDKQIFFVNFYKKEDIYINLHAESVDQSILQATIYIEDDTIEMNQQSMIITANQIKYDKCKKTEEIVQCIIAIHVESSEDTQFTLLISKESQIIKLYNEESITKTIKNQLDFFTFLLTEETEIATFSITANIRILVNIIQLDPPTLEQFPTNDDNSQFQSLNDDIIIESSVFITLNDIQKINCDKTPCYAAVTCINSDSQNQNNNEYTIIRSSGAFKLTEGFSYTGKIKYNQIKYFKVTDYNEATGLQIIVTNSERNFIVIYASVNQMPTQQSHDYSSNFNFGDFLIIPPKKNSKQIVTYYIGVFALKEVTISIQIKLGSSRFYRLNTQKPFYQVFPWNSKTYLSFYNGQQIDFSILISSNAINQYIKPKIHVRSYQINELSEVINSIQTQYHWKMEDLYLEITTNQENFCFNCYFMIYIENNFLDIELTITIARKDFPIQLFDNIEIKNKLKINETQKYFYFPYRHNEFYLNVTVFQGQIYLFDKKNKTDKEFSNLLLVLDEKDGTLDYQKNQIGSREGIYANRTLIINVINDENDLQVYYLKVFSNITNESIYQIEIIGKNQAIELKVGQPQRFSIGQLFFIPFYFVIPNGINNSIEDYYTITIEQINLQRDKSTRAPDITLKNDRYNNPNILSDYHDFVDANISFVQDIDSITYIQIPSIAGLYFLTINPIGQNTFTYCITLGNKDYNILTPKSQRIVRTKVGEQKVWEIHIFEMAKLFIQIQLCGGIVHVFGSASRDNLTMGFYKDKIESVHNQQLSGTIPFVLPNTYYINTQTIKNTTQTDVVSYILSLDILKQDTIVPLNHFYPGNGGDFQISIIENHINFDYSPIQSSEKSSQDYLLESITYIFIYQNQSLNDNTQLYKCNYNSQYHSFTTRRIQHDPQNNLNQRIYIGEDQYEKLLASIQAIVKIQTNNYETLQLSYYYNTQILEQKIITQNIYYENYRGIFIIILSVLFFVFFLLIIKFRNLKQMTKFETLDQQPIPQKEQQIEMNYTNLRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VXR6</accession>
    <name>A0A8S1VXR6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0750142</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000075">
        <property type="protein sequence ID" value="CAD8180985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VXR6"/>
    <dbReference type="OrthoDB" id="10296517at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8180985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="126" mass="14858" checksum="791134FE80E1DEAF" modified="2022-10-12" version="1">MLTKSAKGNQLHQTITTINYRSIINSEKRQDDIPQYMNGRCKIKLRKEKNQLPYLNIREPSLPTLKSPVNKQQQLNSMSWISLIQVQNRPRDKSISIANETYFQRTRQVSFIDNLLKQLNKNNRQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W1U3</accession>
    <name>A0A8S1W1U3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0820091</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000082">
        <property type="protein sequence ID" value="CAD8184004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W1U3"/>
    <dbReference type="OrthoDB" id="416093at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF803">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="8">
        <location>
            <begin position="91"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8184004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="389" mass="43783" checksum="2B80C33327445D8C" modified="2022-10-12" version="1">MSSQQNKQKDVVNKQMIFALNPKPDFNITNPIKINKSNTYDPNYVKSEPSQKTTNQISNSNIGTFQDIEQQKQNNESKCLKKQNKIKYIATRSIAGLKAYTKTLKDNQDSLFYKLEINKQIDKNIFLLADGHGEQGAKVSQFAIKQLSDCIFDMIQSMKTQPQFIEQLNSNITAFFQQIDSNLANLSNIDITDSGSTMNLVLTYENYLISANIGDTRTFYFQRSDKPDQLGPSTLRIKQLSIQHTPEKPQESQRILESGGKIEQETQGFQKTGPLKVKSKKQDINGATITRSFGDALAKQIGIISLPEIHNFQIQQEGFLILATGSILDMIDIIDICRILTPITPPNSQAAIDHAAGQIIEEARKKSKLQPDIQDDYSLILVYIEIDLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W374</accession>
    <name>A0A8S1W374_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0800093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W374"/>
    <dbReference type="OrthoDB" id="293253at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8183127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="65" mass="7278" checksum="6A75AC569F342AA2" modified="2022-10-12" version="1">MDSSAHTVRIQPLSKKKEKDPKGNTKILSSRINFEDPTALLQTRTLLANFIRGNLNSKTNPCPNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W4H4</accession>
    <name>A0A8S1W4H4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0800092</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W4H4"/>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003366">
        <property type="entry name" value="Helicase required for RNAi-mediated heterochromatin assembly 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003992">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF495">
        <property type="entry name" value="HELICASE SENATAXIN ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="1">
        <location>
            <begin position="284"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="2">
        <location>
            <begin position="580"/>
            <end position="783"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8183125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="827" mass="97857" checksum="4AE118FA33D79615" modified="2022-10-12" version="1">MISEYQQYSQPICVLTNSAFQTAIQEGQVDNLKGYQQFKSVEEYQDLVFPLLQNENYRSLKREQFRFLKNLQDSDQFPLVFEVILNEDADPNREDGIIFDVLRELEQMNNEQSDDNEFWKYNHYAQYLIFKQSQQYFGAIYTRNPNKPNDYFTSLAIYQKKKDEIQLILPYTKEVKKLLNFENIFTTNEKLNHYKNVCIENQKKQKYKSSFIWDQKLLIYFIPISPLSSYLNETETIFNLSQCPFYKLLLSPCQQLNLVNASDFSQRARIQQQLYFQNQIFTRQFNENQLNSIRHALDFTKRFTIIKGPPGTGKTQTIIGIISIMSELLIRSDNNSQGGILLLAKSNSVVNDLIRKIQKNIEEENSIIYCFNKKPNKLKVLRFGRPEKCDKDIIRLSLEIRSQHHFFYFKTKKNFKELEDKCITSEIKLIMRQNGLKEFGVYLQETGQQSNLITLLSYIEELNFRTRNQKILEAYGNLYSQWSQLIKNEKKVYEEIEQDYLEQCHIIVTTLNSCSKLCLEKYFEKVKLRMCIVDEAPTALEPSLLIPFVKYKFIDKIVLLGDINQLNPIVIAKESNELGYNRSLFQRLAKGLKQDTLKLIEQYRQMPNLAQITSQFFYKNQLRNGIQNMQFPDWISYKVSNNRNRLFFSAPAFTESDEETSKRNDLECLAIIKLTKYLLNGSTLPQNHQKPITIISAYRAQTENIQKKLRQETFQFNQPEDNVRNLLDYVELDTVDSFQGKENEIIILSLVRSNDKQGFLKDKKRANVALSRAKYCQFIFGTSRTMRQDLKNWNRILKILREQNEIINYNQESLTNPNFFRRELMNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W7Q0</accession>
    <name>A0A8S1W7Q0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0840096</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8184967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W7Q0"/>
    <dbReference type="OrthoDB" id="10023235at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000018">
        <property type="entry name" value="Serine/threonine-protein kinase 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.90:FF:000062">
        <property type="entry name" value="Tudor domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.90:FF:000066">
        <property type="entry name" value="Tudor domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.90:FF:000149">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016685">
        <property type="entry name" value="Silence_cplx_Nase-comp_TudorSN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016071">
        <property type="entry name" value="Staphylococal_nuclease_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302">
        <property type="entry name" value="EBNA2 BINDING PROTEIN P100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302:SF2">
        <property type="entry name" value="STAPHYLOCOCCAL NUCLEASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00565">
        <property type="entry name" value="SNase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017179">
        <property type="entry name" value="RISC-Tudor-SN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00318">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50830">
        <property type="entry name" value="TNASE_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TNase-like" evidence="7">
        <location>
            <begin position="2"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="TNase-like" evidence="7">
        <location>
            <begin position="306"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="TNase-like" evidence="7">
        <location>
            <begin position="492"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="domain" description="TNase-like" evidence="7">
        <location>
            <begin position="652"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="6">
        <location>
            <begin position="698"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="850"/>
            <end position="870"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR017179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8184967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="870" mass="98415" checksum="9247FE52C10CE24E" modified="2022-10-12" version="1">MEKKQGTVKQIVSSNCVLISGPINNETGVPMTKFFTLQGIQAPEFSLTDKENPKQEPFGFQAREFLRKQLLGQQIEFIIDHKIKDQNDKVIGRIFKNGQDIGELQLREGLAQLRQQGKPSQEYENAQNLAKQNGKGIWSKKEELVKYNKIELKDVKPKQYTQCFVEDVQGLFVFYAYIPELQGLVKCSYGEVFMPTSVSQILQNRAKWTIQELILQQEVTLTVQGQDEKFQSLRVDIKKKDLDVKKELVSLGYFRLSPNAFQLINDQKRYNELKETQSQAEIKLIGIWKDAMKQQQQSVVLQGGKQTYTAKIIEVHSGDQLTVMNVNNRQQSRVLLASIKAPKYSLKETEPFGYEAKEFVRKHAIGKIVKVEVEYEKKIKPKEIEGLADEDDKKKLQQELNMIFVNIILTEDNDSNLAALIVGAGFARVQPPRGDDGVSRYIDELTGAQEAANKAKKGIHGKPVQLPKMTDLSVNPNLQRSRDAFDSLRTLRKLSGVVELVLNGSRLKLRFQEQNYTSIVVLAGVKCLPNEQNLPDYQKFSNIALQYVKENALQRDVDIELTSIDKKGIFHGHVFLGKQRINLGLTLLELGLAVTFNPVANSHAYQALFADAESKAKLKKEGLWDIKGLDLTIVKGDDDVPVRSECKLLNGELKKLVLVEIADSNTLYFQDPTDKLLGQIEKSLGSFTAIEANKLIPPFKKGLLCVAKFSVDGNWYRAKITRELKNRYEVLFIDYGNVDIVSQNDIRKLPENLAALPPQAIRCNLAYITGPSISHELGNKVGQFIRDQIFEKEVVVSFEYQDDVSKGVIAYLTKENQANKSLNILLLSQGFAKLDKTAPPLPQKLEEWVKASQEAENSSKGLWNYDEETE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W8V8</accession>
    <name>A0A8S1W8V8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0840024</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8184825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W8V8"/>
    <dbReference type="OrthoDB" id="287991at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8184825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="190" mass="22505" checksum="2A2B5B39B00A5612" modified="2022-10-12" version="1">MSSGESDSEYQEFVTNFYDLVQDINEYYATKSKENRTAKFLKQAKARRKVNQITFRMPENPDQNVSEIISYNKNQAEKLVKPMRHQTKNLENSQVEPYIFNVHLILRDLKLKQQILRNAQKQVYLNLIEYHKQKTKKSKPRNCCLHIYLAEKIYQEKMKEAATCKKFQEGPKIVIPLLKNQAQTRKGSME</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W9R8</accession>
    <name>A0A8S1W9R8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tubby C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0850226</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W9R8"/>
    <dbReference type="OrthoDB" id="8775810at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000007">
        <property type="entry name" value="Tubby_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01167">
        <property type="entry name" value="Tub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubby C-terminal" evidence="1">
        <location>
            <begin position="423"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8185697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="514" mass="61241" checksum="BBEB7CE7796748B7" modified="2022-10-12" version="1">MQSNLSEIKKYQQQREERFHQSLFKDQIQNIDSTIQDDSNNPDFVKVIVICTFTNETIATKEKIICSDNDVQIIEKDDVVEQQDLSECAKKEIVTYIERSFIECCKLLKSNYIEKFDVIKLPICSANGIRIPNEYDIRQFIKLSKIKNNNEEFNKSLRSMDTKHLFGLLISSLIFQDSQIEEALFELLFQNINLENAIKLLLITVQYESKKQHYFYKLIKHLYYFSKGLLKETYDNYLQKIVQNGMKPKQLQVEKPNFIKLITQLHIFKINSTILYPLFKQQESYLFQYYKPIIEKKSFTVHRNLDCQEGREQSDFPHIYYLKNNENQTILVAVQYEISGDILIFQEYQEKFIKYNDNYLGLIEFKNQGKEFIVFDDGYPEYMENSFPQWIGRKKRKMLIFDYFSDKRYDKPRLFDVKCYNINTNQYDEIITQEPHYDDQKGSFILKLKDIALIPSTRNFVLHKKGDNIKEQQIYLCHGKQNRHTFHLELHEGISVFQGFCISVVSICSKGLRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WEJ4</accession>
    <name>A0A8S1WEJ4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0890195</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000089">
        <property type="protein sequence ID" value="CAD8187673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WEJ4"/>
    <dbReference type="OrthoDB" id="316079at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="252"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8187673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="396" mass="46868" checksum="E11DB8F7184FC8E9" modified="2022-10-12" version="1">MIIKNQNLIDRVIDVLRYDQEDRRPLQIFLQKHDLLSQHIGLLMPNLKLIYFDLVGQNVDCEKRVYLFLKGKKAVKSQNLLQCIVQMNRQKEIEAKLTFLIQFSFFSGIYLSSIYAILDQIPLKTFQYKTNLSHVLPGIICIIKSGSVVLQSSQGPFHKQFLIITEKDLFGESHFVDEDSKINLKKQNINQKYYEAICNSQVVELYLLNVSELIHTINSQQLRLFLQKKFRQRIMQLRQSVEQNQSFSKPLQQQQQQQQQQQQQQQSQQRLRTNTQTSDSQYEHLKQKETAAIDKVREVVKNMNFSYSRTRNYFASKLGAGNILFHKAFMYQYTPHYTKKQRQSSFSTAIKQKLSQRTERCKTMSNIEMRTTMLQNSFGKYQFEQKEKQSKHNIFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WGS2</accession>
    <name>A0A8S1WGS2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0910013</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000091">
        <property type="protein sequence ID" value="CAD8188191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WGS2"/>
    <dbReference type="OrthoDB" id="272141at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14137">
        <property type="entry name" value="STKc_GSK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000082">
        <property type="entry name" value="Shaggy-related protein kinase kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050591">
        <property type="entry name" value="GSK-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039192">
        <property type="entry name" value="STKc_GSK3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057">
        <property type="entry name" value="GLYCOGEN SYNTHASE KINASE-3 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057:SF0">
        <property type="entry name" value="PROTEIN KINASE SHAGGY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="34"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8188191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="365" mass="42232" checksum="DD34D7CB052692D6" modified="2022-10-12" version="1">MINKKSSTKQATRMVTQANDQQEVVTQNQQASKYTLNQIVGNGTFGMVYLATNNQTNEKVAIKKVFQDKRYKNREHLIIQELNNPCVVKLKEAFFTQGDKGDDVYLNLVMDYIPDALSKVIRYYKKAKQLFPVVLLKIYSYQMFRALAYLEGVGICHRDIKPQNILVDPNSHILKICDFGSAKKLQKGEPNVAYICSRYYRAPELIFGATEYSTAIDMWSIGCVIAEMTIGDPLFPGESAIDQLVEIIKILGTPTLEQVKLMNPMHKEFKFPQIKSHPWNKVFQKFKPDPLFVDLISKIMLYPPKERLRPLEALSHPFFNEIRDEKFGIPNVQLPNFFDFSKEELQIQPEISFKLTPQWYSQKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WI48</accession>
    <name>A0A8S1WI48_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0900074</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8187849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WI48"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8187849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="598" mass="69063" checksum="BEC0E87865945B1E" modified="2022-10-12" version="1">MNPPPGLCDDDDNQIGPPPGIDIPQKKKFHVNKKQSQQFQQTSLESLNLELKVTVQDQQFDQIPISDDYLEPSMNNLIAFNGYLCDTSGADSLAIWDKEMKLKKEYKGLIENMPYQMVAVHDKIYLIFGDILMMIEPETNKKTILIEQALTAFCVSDHPNPRIIGVQDGELMLWRTSNFGKPNQMEPKYNNFEPKTKKMIELQKVLSEKFDDCDDLLMLQNGLVFGKQKKVGEVFIIDFVNDQLEELQTTYGCAVTCWTPISYDSVAFASNGQIVITKFQSNKQAVVQIILNSSKDDIHHGLCYTNNQLFSISKNAVVTSWNIVLGTKTKTFEVKFCPGVYSLITMNQEIIIAEHKVKKDFILLNTNLEKQFFYKARVKQVLHCCSSPNQQFLAVVQKCGLLYDEEVIFHIEIEIYPFDNKLEFQRRKEIDNLEIKTVMFLNDSTIQCILKISKSVYPLLVKWNFITNEYSESEMLVEKYKSCQLYQNRSVFSYDFHNPPSQQFNLFAPKRVDDQNQSTGISVYDENIKRLLKVQFQQQFNADVFGMPTPDLLYVLNNKNQIEVWDVVTCQQVQKADCRPSIINIYFQMLNQNGSQEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WL08</accession>
    <name>A0A8S1WL08_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0930184</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000093">
        <property type="protein sequence ID" value="CAD8189377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WL08"/>
    <dbReference type="OrthoDB" id="273771at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="394"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="275"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8189377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="429" mass="50365" checksum="9C73F866F6FCAE81" modified="2022-10-12" version="1">MQSVEQYTEQVRTLTQQCIETFDRFMDESYDRVNGAIDSIYEQMYLKPAPEQKKKDQRTSTELVKLPDAPKVKIEVIINITPDETKSFRQKRNVCNNRRCKQQYRKSRRYAQEGQIKDCVACLQSFKNQDQKEKEQIKKIIHILKNIKDHEFNQKDYSSEPFEEMKKNLIKKLQGDKSMIELLTFLVMLTSIDDQFISCGSNSLHMLVMMKVDLRNQSFENIRIKNTSIIGGNFVRCNLNGSEFENVDISGVIFNGAQMFNYKWKNIKVHELNRLDGHSSCVRSVCFSPDGNTLASGSDDNSIRLQEIKKKYCQDYRFKEIQVKNTRYPFLKPLCNTSNIMVYPDFNVQRFKIVFYKKPLPFKTILGVALDQFKNQPILTLTLNVNLFQQTSCAQYSLLLFYFIVFSFLTILKFRHYQNSNQLAHLKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WMA9</accession>
    <name>A0A8S1WMA9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0940178</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WMA9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8189741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="142" mass="16678" checksum="47DA052B99E81383" modified="2022-10-12" version="1">MPEANQWQLVQKVLIFGILSSLISSFGRADYNLPLFIFAIFLWEFEKFHTRIIYLLLFSFIIDFVYALYWHNQWSRFKILETKIDSLLHSIVILTAMLNMIIKIVVILYSVGNNNEVKHNLFPGAIKDNLINFITFKNTGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WNF8</accession>
    <name>A0A8S1WNF8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fatty acid desaturase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0950167</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000095">
        <property type="protein sequence ID" value="CAD8190161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WNF8"/>
    <dbReference type="OrthoDB" id="1461976at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03507">
        <property type="entry name" value="Delta12-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012171">
        <property type="entry name" value="Fatty_acid_desaturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32100">
        <property type="entry name" value="OMEGA-6 FATTY ACID DESATURASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid desaturase" evidence="2">
        <location>
            <begin position="61"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8190161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="358" mass="41048" checksum="C6CFAAAE7539303D" modified="2022-10-12" version="1">MSKKELEITLGQIRKAIPQHLFQKNELRFLISVVQAISYTLLLTYIAYNYIPLNWLALPIWIGYAFITGTVATGIWVLGHECSHFAFSDTKWLNDALGFLLHQALLVPYFSLQHSHSVHHAKTNHLTQGEAHTPVIIDSKMGKLYQKMKDILGEECFTFIQIFNIAVLGWPLYFLVGVSGGSARGFTSHLIVPNKLLPKKILHKVAVSNVGICLVIYQLYKWYLATSFAEVMALYIGPYLVVNMWLTIITSLQHNDLNIPHYDESAWTWLKGNLCTIDRNYPLWINALHFETGTTHVLHHLFSELPHYNAREANIYLKQILGDLYYQDTKNFWISLYQTASLVGVEHKGNGVWKFVKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WPC9</accession>
    <name>A0A8S1WPC9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0960147</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000096">
        <property type="protein sequence ID" value="CAD8190491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WPC9"/>
    <dbReference type="OrthoDB" id="10412283at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8190491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="224" mass="26504" checksum="815D1D15C9B08A23" modified="2022-10-12" version="1">MQFYNFDNENSLPQIYDDNENQGSELNYIFQSNQQSQQDQENLLSQNPILQTENSYNQQNFYQQLDQEQEPEICHQSQKSTIIARKKTKIRLKQPKKIYKEEPIAQECEKKESTQQSETKNLPKFYANSILNKLENLNTICQKQNPQIKKKIKKFDDKPSNLKTLREMLQDPIINKITIEFITSFDFLDQILGSGRLQDVKPPITYLNKLYQGCLNTDVLKQWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WQJ6</accession>
    <name>A0A8S1WQJ6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0960131</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000096">
        <property type="protein sequence ID" value="CAD8190459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WQJ6"/>
    <dbReference type="OrthoDB" id="296210at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="450"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="491"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="558"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8190459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="607" mass="68639" checksum="257309F8DFB3295C" modified="2022-10-12" version="1">MFDNRNQQQPSMFGVQQQQQQQVFPPQYQGVDPYVQRSPPKAQGTLGFQQPQQSSYVQQTPQQGSQTVYEHKVITTGQVQSPYIQQNPVAFQQVSTIKSKGSYCIDWCIYFIPFLFFIAILAFMGMIIAQKDRDFNQEPINNVLRTFSHNQALTPIADIVAPHIARSSSMHSHFQHKIETELCPEGFTLATLGEWPGINSGCICDKQIKKEGFCWARSGCDSIDSQDSKYFQTWNGGRVCQKLTVGWTKLNGNTCSEGYKKCGNVCVPTNGDASRMCPLSDLTLLSTDPTTDSDPTSLNTQVVKIGNKWYRKSYDGIPIVQFQIIPGNAEQVKNSAPCLNDITTSTFESQQSYPLLKKPATGCDQFGNLQNEFVKIDTLTAKKVHEENGILSSLHTIPGYSSYEVESDSYTLEGIRRISVNPTIECSSISSADVNQIIEDSEDIYQQRKWFSIIIFWICVVGIVLFVYLYLIRSRNFQTLNVSQIKQPKILILIAVVVAILCIAFGSLYLYNMNGDDGLRNVDSRFKSQLDNECFNDKGIKNSIQQLHNFAYSQYSTIGYWITWAFWLSIAFLILMIILIVIQSGSHGNLCINPWQSNWVFGYNEFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WR07</accession>
    <name>A0A8S1WR07_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1010008</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000101">
        <property type="protein sequence ID" value="CAD8192173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WR07"/>
    <dbReference type="OrthoDB" id="320994at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8192173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="100" mass="12171" checksum="04266DD422E91929" modified="2022-10-12" version="1">MIFGIIRRERRYKHVNQLEYQGREGFIWNSKQRSCQFNLVYHQSDEKMISVLNTGVQILTIDKQNKKIQSFDVYFGGVIRTFNYVAIHNNVQFFFWNKDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WW85</accession>
    <name>A0A8S1WW85_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1010138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000101">
        <property type="protein sequence ID" value="CAD8192409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WW85"/>
    <dbReference type="OrthoDB" id="306942at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SPRY" evidence="1">
        <location>
            <begin position="74"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8192409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="191" mass="22207" checksum="63761CABD1272AE3" modified="2022-10-12" version="1">MCQEQNEKNEEMLKQLQQSLVQQFSQSLTFSTIYKNSNCSVTQNGKLIETSQYGWQCCMCDQMIPKNGLIQFAIKIIELSSIMIGIGFRDIVQSRNYGYCYDIGQGTYNIYNNGECYNHDQQDKNNKQIAFPFSTNDIIIVEVDIQKKYVKWTKQSTNQSFTLNIDTSKDLYPCVHLHSRCKVEILNQVFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WWC3</accession>
    <name>A0A8S1WWC3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1060043</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="47802at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000320">
        <property type="entry name" value="echinoderm microtubule-associated protein-like 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000898">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001719">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055439">
        <property type="entry name" value="Beta-prop_EML_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005108">
        <property type="entry name" value="HELP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050630">
        <property type="entry name" value="WD_repeat_EMAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720:SF33">
        <property type="entry name" value="HELP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720">
        <property type="entry name" value="WD-40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23409">
        <property type="entry name" value="Beta-prop_EML"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03451">
        <property type="entry name" value="HELP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="49"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="85"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="134"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1170"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1277"/>
            <end position="1304"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1514"/>
            <end position="1547"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1978"/>
            <end position="2007"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2235"/>
            <end position="2272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1573"/>
            <end position="1601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8194138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2272" mass="254880" checksum="085A51885AD99843" modified="2022-10-12" version="1">MGNQIMGKFRKNRFQGFSDNQIDYLSDKWLQIESNGVIDPNKFADQYNVSHKEAIKIIKLMDFDDSGRVDYYKFKVGVASLCQNPMTATAKTIFDLYDEDRDNKLGPKEVAIFLTSILNNLHYYEGLDDPTPKEIEEKLQQYLVKWDLDRDGGFEYEEYIKLMQKDPDVLKVLYNMGLANKDEMTIEETCYNDIDSDIEIEMERRLLPRDERVERIKNGIEHTVSAQATDEFGLEEEQVVMEWKDQAKRLEPTKKPEGMDASPPNAILDLEYVYGYRCHDTRNNLKYGNKGQIVYHTAGVGIVLDPSNMTQKHFLEHNDDISCLDIYENLVLTGQVGLNPMLMVWNIDNMRMVCLFNDVLKNGISNCCFSNDGKSVAAIAMDDDHFMAIYDIEIAMNSRKDPKNKVSPLIASGKLTKQEIFDIKFLPGDWQIVVACMKEISICTWKNGTILAERGVWKEQQPQPVLTIAINGGYIVTGVFNGNFLCWKKNICVKSVKAHATPVKAIMTRSAGRGIISADKRGIIMGWDPQFNKVFEIDTKDLPLKLSYPPKGSPNVISVCESPDGKILFGTRRSEIAEILSYEEGQKIQCKLLMQGHFNGELWGLDAHPTQNIFYTVGEDEMLAMWDVKQKKLLKTTPNQYASKTLSVSQNGKWIATGCSNGRVFIYDASSLKKLQEITEVVDPDKEIISLIKFSPNNELLCVCYKPPFSEIAFYSTKTWKKQSKIPNCKYHVFTLDFSADSKFIQLNTSNYTIEFYDVNSASPVQFDIAKDIQWFTWTCIYGWQVQGIWPDCSDGDDVNATDRSKDGKCLVSCDDFGKLKLFKYPCPKEKSGCIKYTGHSSYVCGVKFTNSGEHIISVGGDELSIFQWRYIFSPSRLDVQDIQDEPEKVEENAGGALFEQEELDKGDMIGAVKPFLGEVQHSVPSWYKPNKARDNQEPKGNLSLYHVHGFRFFYILDECRDMLGWTDKNKVVFVSAALGVEVDPKTQQQAFFNKHEEDIVSFALHPNRNIAATGQMAQAGKAKCIDIFVWDIDSKEVLANFNQFHLRAIVLLKFSPDGSLLLTVGQDDDNSLAIYDWQGKRQICTSNIDKAKVNGAAWKDNEEFVTVGNKHIKFWKISGRNVQGKMGQQQGKFESQFSVVYAFDNQVIVSGGGSGTLFCWKGGSSDKGIKGHEGKVCSFIMDKPKKLLYSGGLDGKVVSWGYEGGQLVKKQEIINLATNPLFPPGVVAMDYNEKTQQWLFGTNGAQIFSYDPTKKQTSIVVQGHFGEELWGACAAPTGHKYVTGGGDKTVRVWDIDQKKMICVSKPFPSEVRAVDWSSDGKFIVCGDLNGFVYLLDPNTLQVMDTAKTIFTTMPKRQSTYWIDDLKISPDCKKVAFGAHGGASHLEVWTIEYPKFGKQGTKIQCGLTSALTALDWSVDSSIVIVNSGAYELKYVDVNAKKNQPSSSQANTEFASWTCKLGWPVQGIFPSADYSDVNTVCRSNSKKYLVSGEDTQHVVLMNYPVVVPKQKRKEYIGHSSHVMRVRFTCDDNFLISVGGNDKSVIVWKTDFGSAQGKVEQANLQELCKEEGADDIDMPVKKKKAQPPPKKQIQQDENSVFSQEETDEGDQFMACKPWMGAIKEPTYQYYQSKTEGHKPPKCDLEIEYVHGYRTKDMRNNLFFLANGQVLYNAAALGIVLDVGSNTQTYFNKHEDDITAIDLNPVDRMTVATGELGAKPNIFVWNAETKEVKCQFKAPLQKGIIAMAFTPNGKRLVAGAVDVDHSLAVFDVSGKGAVLWSDKSGPDVIVDLRWNTDDAFVTVGVKHYKCWKYDNGKCQGKKGQFGKGASNNLSGIAINGNDTLCGAADGCIQVWKGEAFMKPLPSKHAQICDCITVTQDYVFSGGRDGVFFVLSKNYEEILAVKVKEKCPDSVCPSVRAIYADLPNSKLLIGTLGSEIYQFSWDGGAINSQTDFQVTNLMRGHFCPNLKWTNEVWGLDIFQEDQDKFVTCSDDGTVRVWSISDRKQVKIGSTLLTADGKEEKRDLNTGDFTDQCKARVVSTNPKDEGFTAGMKDGTIRVYDAEFNQTKVFKQAKEWISDIKFSPDGTTCVIGSHDNALYAYKYPSWKAIGKKMQKHSSYITHFDFSRDGVNLHSTCGAYELLFWDVAQCKQLPGGASALKDELWYTWTVTLGWPVQGIWPECADGTDINAVDRSNTTINGKNDPKTSYHLLASGDDFSHVRILRYPSLKKSSEAVVGTGHSSHVTNVKWTKDDSRIISTGGEDQCVFVWKVTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WWI2</accession>
    <name>A0A8S1WWI2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1050050</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000105">
        <property type="protein sequence ID" value="CAD8193802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WWI2"/>
    <dbReference type="OrthoDB" id="306719at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802:SF155">
        <property type="entry name" value="DEUBIQUITINASE MYSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802">
        <property type="entry name" value="SWI/SNF COMPLEX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="469"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="368"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8193802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="513" mass="60824" checksum="B11D66A7510CDD1D" modified="2022-10-12" version="1">MNPYYSYYPNSQQNNFWQYYQYNQLPYWYQMNNQQQQQIMSYQVGCQSVPQQDQSNIQSNNLHQMNDLQNYVDIQSLERMKTQNNIEQTNLKVDQDSKKKVKKQRGHWTNEEHYKFLQFVRLHNDLFKTTVHKKLNRVFKMMAEEIQTRNACQCRSHFSKFNPFIETNILRRKLQIYIKLYKLINIYKNNICHNFSPRCFETQVKVNSKCQQSKALPDDFASQILNNDIVLVLSNYSNNEALKNLIQLYMQGVEYYKECEDRAHEYFEIKLKNIFTNPSVLQPTEQAKQSTPPEKKKIEVPKINVVQASYSFMNKPKTSKKPDNNILSFSFQEPVVDKFFQCGSTINKNSNSQSFCKIIKNTESSHSGEEEEEEIIHQKNIIEQEKEEKIKEIQNQVIEEQIIIQTEGRAKEIASQFKKQERRISQQIEAGMNAQESSIQKRKEMRKLRSQFSLHNINKTQSTPTIKQFNQHKTPQDDPNCIPEETNEIGSGKLSSNKKHIEVNQFQFNDECK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X013</accession>
    <name>A0A8S1X013_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0990118</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000099">
        <property type="protein sequence ID" value="CAD8191596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X013"/>
    <dbReference type="OrthoDB" id="304096at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="377"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="132"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="310"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="384"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8191596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="496" mass="58964" checksum="60E48C92E9B732D4" modified="2022-10-12" version="1">MQNTLFDFNQDEQNIKKIIDKDINGKTQLVFNVEKYNSQFQVLTQDCLQQCERTTSQKGAKEMIEFKAQVISHEKVIEAVRSANFVEIQNVNQRFKRLFKEILEYISDQLGNVYKQEYLKLWLECKNSKAILQDKELRLKSSETMIQIQEQEIIKLRTQFTSQNTFLQLNKKLEETINNLEKELNHLKESLHNQLKINNDLKFNFEELNKKYSKLQQSFEEVKSNYEKEINQLKLQEINIIKANKDLIELTNQLSSQIDKYKDLYEQQNQKNLDLNDQVKYWKSTHFQRLVDLQQSEQKIGSQKGYLFKYRREVQDLKRFKLQNEEVQKELSNARQTVHILNQQTEQLRLNYESAKTQNLKIQIEKPDNINQILIKNSQSTKKTNNDSIHDSPKKGKFDFGYMKKSVQQQTPKEEITQLLINNSKLPIQLKQKNRSASAKKNIQLETDTSKNNIVLTEFDQMTPFLENNLQQFTFSQAQRFPQNLKKRIKRINTEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X1R2</accession>
    <name>A0A8S1X1R2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1080006</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000108">
        <property type="protein sequence ID" value="CAD8194881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X1R2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8194881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="263" mass="31517" checksum="0A62E0B63BD8806E" modified="2022-10-12" version="1">MEYLNKISFNYPAPHIKNFREEKFCTDKNSKDYLSYTRQGYQRQMVIVWFQNFGFYESTIEQTNQGFSKKVMIEEKIKDFNGQIYFLKSLNQKMKEKIIFSFEHQVIKLGQTIKQSIDLILDTQIDTNIIGRNIYNVFGHDSVLSNDCNLFEQRIQKQFLLQSIIYLIGDQYKMKYVFKNQRIQSRLSDLMTGRVNVQFEIDFQIKFRQTEISDPSVLLSLKKHQKFMSNTQDIIPEEAILVKSENRFSIMKKRRRQKQENRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X2V3</accession>
    <name>A0A8S1X2V3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1120154</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000112">
        <property type="protein sequence ID" value="CAD8196484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X2V3"/>
    <dbReference type="OrthoDB" id="295796at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8196484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="97" mass="11549" checksum="13ABB8C813851FD5" modified="2022-10-12" version="1">MQEYERVKFIFTVLEKFIVMDLNSKIALDDLKFTFTNILKIDLFAKRLQFSITKGLKQYSLNSQQSLIQQENLRIFNLNNQERKQIDINVKFIEEGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X3H4</accession>
    <name>A0A8S1X3H4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1090161</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000109">
        <property type="protein sequence ID" value="CAD8195521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X3H4"/>
    <dbReference type="OrthoDB" id="414590at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034464">
        <property type="term" value="C:BBSome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056335">
        <property type="entry name" value="BBS7_hairpin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056333">
        <property type="entry name" value="BBS7_pf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056332">
        <property type="entry name" value="Beta-prop_BBS7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16074">
        <property type="entry name" value="BARDET-BIEDL SYNDROME 7 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16074:SF4">
        <property type="entry name" value="BARDET-BIEDL SYNDROME 7 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23349">
        <property type="entry name" value="BBS7_hp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23361">
        <property type="entry name" value="BBS7_pf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23743">
        <property type="entry name" value="Beta-prop_BBS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BBS7 beta-propeller" evidence="4">
        <location>
            <begin position="25"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="BBS7 platform" evidence="3">
        <location>
            <begin position="468"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="domain" description="BBS7 helical hairpin" evidence="2">
        <location>
            <begin position="563"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="324"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8195521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="674" mass="77712" checksum="BB6AF9C93328617B" modified="2022-10-12" version="1">MDNTLLQSEVFRLQNLKNVIRAFPNNNKLQQLLVADDQGNLYLLEANGDQFKIENKQQISTGKDINYLDLLISPATSEKDRIIVTSGSQVLGLTKKLREYLKHDTYSADLIQFAMIEGNQLFTAGEYMLNEYTYQSDKVGQLCFFLSPGKINCLQVHYLSSKAHPILGCQDSCVRVLNQDQVLYVVTMTAPVICMHVYAPQYKEISSQNRIEHRRCFVFGLEDGTIVAADLGIERANILFTFKVTSIASIKPYDFFKTGKTDLIIARQDGVVEVYSDDELRVQKKINEGITSIECGQFFNLNGPTEIIISTFQGRLLLLREGQSNSIQKNQKDIELQIKQLKTEIAELQKQQMKDNQKTGNVSRQNNNNKVQVKFKVSHKLIKNDKNGSYKLIIDCQYPIDIIVLNGQKSQKCYTIRDLKVSNYEISITQSDIYNIFVIPIDINSSQSFSINVKSLGQYQSINLMNIKNIPLSTLTLKGEFTKQDMIMWVNDLTDLQNSQEDAQTHLFRNSTLDSYLQIKFKQGHALFKSDSISAIYTCRQSIMNKANERGIQVEVKWECKNESLVQYLQKIKNLYEDYIKIKKNYEILPAIKELLVDNSFSILSKEFQQIYSSEQELQEIYKELPNNIQYLEDLLIDTYTSKCNLKSQFDMNIDNVKSLWKRDFQELIQYILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X849</accession>
    <name>A0A8S1X849_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1100155</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000110">
        <property type="protein sequence ID" value="CAD8195816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X849"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8195816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="180" mass="22421" checksum="97CC217451DC07CA" modified="2022-10-12" version="1">MKKEKKQKQIQKTMRQMIFQTLMIKMIHQKKVIHYLIQIINLFQQMQMFKFQEKKNYKDLLLLIFTIIRYKNYYQDLIFQEIQIHNFLKMVILALINIINDSKQLMSKWLFLDLKNRIQKVLYFSNNQPILRIEYDDQSNFFLYDTFRFNILIPFNFKKLQHRQIKKIEIQNQFKILLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XAJ6</accession>
    <name>A0A8S1XAJ6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Autophagy protein ATG17-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1180021</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000118">
        <property type="protein sequence ID" value="CAD8198226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XAJ6"/>
    <dbReference type="OrthoDB" id="304292at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990316">
        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034517">
        <property type="term" value="P:ribophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040040">
        <property type="entry name" value="ATG11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045326">
        <property type="entry name" value="ATG17-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13222">
        <property type="entry name" value="RB1-INDUCIBLE COILED-COIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13222:SF1">
        <property type="entry name" value="RB1-INDUCIBLE COILED-COIL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04108">
        <property type="entry name" value="ATG17_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0072">Autophagy</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Autophagy protein ATG17-like" evidence="3">
        <location>
            <begin position="148"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="252"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="392"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="517"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="602"/>
            <end position="839"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8198226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1032" mass="122529" checksum="FE13109B4449A0EA" modified="2022-10-12" version="1">MDFIIKVGIDGHQFLLTLSPENKRLQYLQEQIQNETKVEVKNQLLLTKKGVRLEDLNQFDVDQQLQQTVQQFQDEQKFYTIYLFDLSENPEQVTFPFPKYEPKMDLKQLLLSDQAKETLVNLNTNLLNHEKIFFQHLQNLKNKYIPYQQKVKQLEKIGQDLENQIQSFDIVLKMSRTTYKTTKAKKEELTKKQKESLERNLQTVKKFDDSLEQLKKIELHAALQTNSQKYLSDIYYKPESMLKWKNSCLNTQQNLKQKIDKLSQAISKSKQKIKNEKTENMTQCLSSYKANQQQYQQFIQENKSLLQSVVQSTIDEYNTLRQYMIDFSQDINDEPAMYYLSQFELSQEQLKQFEDAVIHLGDHLEKTKQVFMQFNQLKQQNMSKILKVFQNLRALDKEFEQHNEKIRQYDEEVEKMEKDFSYLLNPVLLPQAYQQALIETSRRKEFRDIFDKKIKSLMSIIDQEKDKRKKFLQQYGRILPQDFIGQLKNLTPSIQIINAFVDAELPNITGLHTDTFKKRQDNDLDQLKQQIEDLNQQCKQTHLFYQEKTTKKSSTYVELLQLTAKNSMKMFIQRAENKPLSSIDVNSDKSQRENDSKLINLKNEFSEQLNNQRQEYEKLKIKLQKQEQDFESKLVEIDTKNSLITELQQKLEFLQNNTIKLKDDLNKFVSKCENLEINLIQKNSEISKLQQQQKQLNDQLTQSKQIQQDLLKYKQSFQQQYENQNDQINELDLLNKQYLQQVQDYYTQVETVQNENEKLKQKIKMLELERDNKQIELEKQNQVYNELLEDYIEKNSLLENLNEEQIKKSQKDQKLEEDQKSLQNKVDNLDLALQQKNNEIILLKNHDQLQLLKVQDDFKEVQQQYEDKINCLTQENLEMTIQIQSLKGQIEYLNQKQKSENIIEVQQKLKLSDPQFLFDLSSVQIGKQSIFIPAQNDVYVPLLLKNIQISNISDDSLSNTSIDSSIKQKQYRVQLASIPQTLREFIIDTGLLFISEVSNITETEEYFQISVKQPNYIIGFEGQDNQIKSFTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XBB5</accession>
    <name>A0A8S1XBB5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1180063</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000118">
        <property type="protein sequence ID" value="CAD8198308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XBB5"/>
    <dbReference type="OrthoDB" id="300546at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8198308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="555" mass="67026" checksum="2A43C37EF95AD7F4" modified="2022-10-12" version="1">MKENLKTEMSDTTFRREATFSLNYISSSQIFYENRRPSTKKSLYLRTSQNQVRLPHFQTQIIQRNEQCIKTEKNNTIISGKTYITKAMEHQYLRISSENLIAPFGCQIQCTSEFQLMISQIHPFPTNYNCEQVINNRGFIVSVIIGEFIYISIITRKDSELIFNLQFNLQFEDNNQRIKTLQDTPLTDFQQMLKFSHSKSFQCIINSNKEQAGIKKNRYQDEISRSERFLKKLANQEKIITKIINDKKTQKVIFEQQWAIIIYFIKMSQRIFDVFWHKKQVMKKNRLVAFRIKSLLRQMKKKIICPKGEFLETRVLVDCFISIKAKTKTIRRIIRNKQIQILLPCLQQRAAIYCIKMKMIRLGDKLNIIKKHILYFEENYTKYKSDMIVKWDNLNEKIKDEEIKWKQAALRNKGLVTWFQVLKDKEFQQQMRKCFIFELMRDRIRQHIKDQQLIKKNRMDLKYYKQKLRVCRDFVEANTYRGEIFRLTNDIYAMHMKNQLFMHVDVEKYFHLLVSKYIILVDEPDNLPNELQIQQKQKQRQSRLTIRRITKKSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XBV4</accession>
    <name>A0A8S1XBV4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1190008</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000119">
        <property type="protein sequence ID" value="CAD8198458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="298777at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8198458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="808" mass="93896" checksum="C8C1CE7C43BD27F3" modified="2022-10-12" version="1">MEQFQECKGISLLKRDQFMYSDSNSELLNNFDQIKYLKFEGQYGQNNRKSGKWKTIWHGELLKGLGGYYENGLKIGLWNELIQNYQNLAQVHENGKYLNGQKYGMWKYIYKNRNIGGGLYNEQFKKNGIWIELSKGFCDMSQVTYKGEYQDGKKVGIWDILFLQELIGGGLYDNNGSKSEKWIEVNERFSGQSQIIYKGQYKNGTPVGIWETWNKRDYMREFQMICGGQYDEGGEGKKXYLKLLFKEKYNSNQYLQNMDFKKQYTRICQESLIQKKGIIEIEIDQQDYINNQLGKTLIQIKQNSNRQIQYIKNGAILRMDQFMYSDSNSELLNNFDQIKYLKFEGQYGQNNRKSGKWKTIWHGELLKGLGGYYENGLKIGLWNELIQNYQNLAQVHENGKYLNGQKYGMWKYIYKNRNIGGGLYNEQFKKNGIWIELSKGFCDMSQVTYKGEYQDGKKVGIWDILFLQELIGGGLYDNNGSKSEKWIEVNERFSGQSQIIYKGQYKNGTPVGIWETWNKRDYMREFQMICGGQYDEGGEGKKIGKWIEFNDVLNEDSLVSYSGQYNNGNKAGRWDIFFEEEEIGGGSYDEDGKGVKLGKWIEICDKYDKQTQVSYSGKYKNGNKAGRWDILFEEEEIGGGSYDEDGKGVKLGQWIELGDEFWSESKLVYNGSYKNGNKIGRWNIEYEYEQIGGGSYDQEGEGLKVGKWIELSDSFNGCSEVTYVGEYKNGIKVSRWDIEYKGKKIGGGSYDERGEGIKIGNWVEVIDGFSLGSEITYEGQYKNGIKVGKWVEMNMRKDQQPKEKIYED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XDA8</accession>
    <name>A0A8S1XDA8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T1200070</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAPRE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000120">
        <property type="protein sequence ID" value="CAD8198919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XDA8"/>
    <dbReference type="OrthoDB" id="2119228at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1430:FF:000011">
        <property type="entry name" value="Microtubule-binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000028">
        <property type="entry name" value="RP/EB family microtubule-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004953">
        <property type="entry name" value="EB1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027328">
        <property type="entry name" value="MAPRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10623">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN RP/EB FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51230">
        <property type="entry name" value="EB1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="11">
        <location>
            <begin position="4"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="EB1 C-terminal" evidence="12">
        <location>
            <begin position="165"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="136"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="136"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="149"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="251"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8198919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="268" mass="31266" checksum="67E94BC712B37E7F" modified="2022-10-12" version="1">MNIEISRAELLQWVNDTLKLSLQKIEQLGTGAVYCQLVDAVHPGKVAINKINWKAKQEYEFVNNFKVLQQAFSKLGIQKPIEIEKLTKCKYQDNLEFLQWLKKYMDQHIVPKDYDPQVKRGNVDLDDQTTIIPKRNPERSRTSIRRDTFSSPNQLQPLTSIPKTNSFAHIEPYQKKQPSMQDLLIQIEILKQEKDYYFQKFKDLDFFIESASQLTQEQMYKGIKDIFYYTADKSVIVLPNGEIEVQGLPPSSEEQSSSQEVIQQEIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XG98</accession>
    <name>A0A8S1XG98_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1230135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000123">
        <property type="protein sequence ID" value="CAD8200127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="292036at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8200127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="228" mass="26288" checksum="BCC8E5EC082FC29E" modified="2022-10-12" version="1">MVIQXCEEFIVFLLALITTAFSQGIYILQFFYLIGVRVVQNQKTAKLLSSKFINDFYAISFLGRNAALSTLLDSSAPFNVIIIPNTRIGRYIFPHHAGKAMSNLVKSYFFQFLCEDLPQTLLQMYFVVSQAIKRSKELQVQVYISICTAILTALLSFYKFLSIRPTNLLQDNFDELSSRLSKGYNQQIQTCLKQENDQIKTYIDLYNSPDYEVVHVEQSQEERQSLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XGQ0</accession>
    <name>A0A8S1XGQ0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinetochore protein SPC25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1240139</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000124">
        <property type="protein sequence ID" value="CAD8200440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XGQ0"/>
    <dbReference type="OrthoDB" id="303140at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="56"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8200440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="222" mass="27054" checksum="9A695C123AF0D77A" modified="2022-10-12" version="1">MNNNIEQKSQFQILQFFEYQNSIQNEIEKSKKILQTILDQKRKLYNDKILQQKEVQMQQQQQIDQYKKVIEEQQQKLKSKGNKENIQTQLGQLISYQQEQNQKIETLIEQSCNENSNYIKLQEKLIKCQNDINYLKEEKEMKEKLIIQLQQITGVYLEYDYHQKILDIQLEYNQNIHIQIKLEDKLRIHTITPKNEHFYQLIKEFNQDNQLDKLIQKIQSFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XHT9</accession>
    <name>A0A8S1XHT9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1240104</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000124">
        <property type="protein sequence ID" value="CAD8200404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XHT9"/>
    <dbReference type="OrthoDB" id="415460at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47823:SF9">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_10, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47823">
        <property type="entry name" value="ION_TRANS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="276"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="318"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="349"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="7">
        <location>
            <begin position="468"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="756"/>
            <end position="807"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8200404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="843" mass="100385" checksum="40D773638D39DD7D" modified="2022-10-12" version="1">MQVYPQKIKEDQFQQQNWSSQRQLTDMGSQLGILQSNSTLLKPNEFSKRIIRAKTVKSFIQMTTLTMSSLQQQRLNKNQELRKIIKKVGMAQKFINQLRNYAYQLKNQRSKYQYRKRGLLPFFPDDIIYIIWGLGINLCTDIAAILYPIEIAFEYEGVGHQLTFILQIIFWIDLLMNFIICHINKQLDLLHNLKDIANYYLTTWFMIDLLSVLPDFGLEGLKPIKLLRLLRFFFYERRVEYNQSIELLRKQQSLRDELIVRDEYNIDLRIKKLMKIFLEMIILNHLFACLWIWICKFNVNKNWMERSDIKYANDYTKLIYAFFWAYQTITVIGYGDLEAHNFDEYLLSVIWMLIGVGYYSFTIGNITFILIQSNPNQEFDDQLLNLEDVTINMPESIQNDWIRFTKYNIQRNPFWAEDSKRIISELPHQLVLYTVAAVHKDILRIIPFMSNDINFSGSILPHSTLVCYQKYETIYHIGQSANDFFFLIKGDIRLCDNYGETLVRVMEGTCFGEIECIENSLRRWSAHAIEESVVLMCSSNYFSQFLEKESPQFFELQQMYKRRKILIIEQARIKRQRQSKLKRGSAINDVHQSNNKRESSQHLQELRFKIDLREYSSVQQDLMLQIVGQKQARNKIIREKFINAINKIRYYVQRSSKIKRISIHDPDYKIVRNLINNRIKDCAWEKLIQKKVGQKLLKNFEKVREDENLIKAFLQKSKQESNDRLARKKLIHIIRNILKFQKKEHQGINKIDIFYNKFHQLIYENAKNEKQEERKKQKQKLESIKKCGALEDQMKNLSSNLKKLVNLQTSLSMSIFEINQQEYDIDCLIADIKKALLQAKIGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XNF8</accession>
    <name>A0A8S1XNF8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ras-related protein Rab-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Small GTP-binding protein rab1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T1310083</name>
        <name evidence="12" type="ORF">PPENT_87.1.T1500080</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Protein transport. Probably involved in vesicular traffic from ER to Golgi.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000131">
        <property type="protein sequence ID" value="CAD8202527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000150">
        <property type="protein sequence ID" value="CAD8208326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XNF8"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01867">
        <property type="entry name" value="Rab8_Rab10_Rab13_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001018">
        <property type="entry name" value="Rab family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="200"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8202527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8208326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="210" mass="24434" checksum="FD5F667CC57B4682" modified="2022-10-12" version="1">MNSNSDYHFLFKVLLIGNSGVGKSCMLMRYSENQFTNNFYNTIGVDFKTKTIALGEHNVKLQIWDTAGQDRFRTITCSYYRGAQGIIIVYDITDRESFENVKTWMAEIDKYASESVNRLLVGNKADITERREVSYEEGLELSRLYQIPFYETSAKSSINIEAAFTHITKNILNRELHNTRAVVRKTSNLRLQNRQQQQQQEKKKQEDLCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XSF9</accession>
    <name>A0A8S1XSF9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1360011</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000136">
        <property type="protein sequence ID" value="CAD8204089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XSF9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035824238" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="17"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8204089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="276" mass="32190" checksum="526FCD3C2E4D7EEC" modified="2022-10-12" version="1">MMSILTIALCITFITSTTFDLYQICDCSQLIFQYDCLSASLECNWDYDNNECYDKPCSEIYYQNACLKQLKRCYWAQSSCFNFTSCGQMLESKYNYDCQGQNYYCPQYYQYYCLSIKDVQVCPSITDPDICNYYQSLQGICIWNGQSCTLAQSCTQFFNNGSSNCPWEYCQHSWDPSYQQEFCSPNQYSDLKTQSQCAQGIQILGPYLQNIIGCYWNPIVNLCQERVPSQMNYANCYLYSRGTYYWNSKTKENGDCVPCSQFQELLIISIFITILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XU90</accession>
    <name>A0A8S1XU90_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1380078</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000138">
        <property type="protein sequence ID" value="CAD8204760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XU90"/>
    <dbReference type="OrthoDB" id="10251741at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001468">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47822">
        <property type="entry name" value="CARBOHYDRATE BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47822:SF2">
        <property type="entry name" value="F-BOX AND WD-40 DOMAIN PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="189"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="381"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="410"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8204760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="791" mass="91736" checksum="11E376580C20D2C3" modified="2022-10-12" version="1">MDPKQRKDRSTSRDKKKTNKKQTQYSYGQLKFGEVQSKSKKISLKTFFGNPEFEIFCLRFDFEDQLIAAGCSDGTVKVFNIVSGKLAFNLQGSSEGASPTTAIRWRRDVANKAKSVFLSANSDGSLMMWQAQTGKQLFRTVEQGNQLLALDIRSDGDQFATAGKDFKIRVYDDEKKEVIHTFDKADYNQPGHQNRIFALKYLEETPNILLSGGWDGNLLIWDLRDHKSIGTIYGPNLSGDSLDFRNGQILTGSYRTNNQLQLWDFGTRQLIQEIQWDKNKNDSDFYVYSCQFSKINGDTILAGSSGRQELKLFDINSQYQTCGYIQDLQEEQQICIWWRRRCSLHLFIDQFKVMNKQQYIQIMDPQQLVDNLNDILLSDFFRYIEVLGRGSFGIVVAAHSQELDKIVAIKITPYFEQENESSLLQECTHPNIVKLYKVLVAYNFIYLIMEKLNGTTLDVILKEQTLDELQIRNIMLQVLNALAFLHRKGIIHRDLKPENIFISNGNHVKLIDLGLGYQIVCRGIIGQQVGTPYYIAPELIKGYEQTQAIDIFSLGIIFYQMLCNNLHPIWTEGQTKKDYYKTLCHEFHIKYPSHLSEMAQDFIKNTITHSPIDRMTAEQCLEHPYLLGEERKSHPITNKEIIQRYKFIQKFQLIVKALQMTKIFKQQCYEGYSYLKSQNSDEINEILLHDDAPKSQRIHTETHIIVRHQKSYYKPQSLKTIHLTKVTSSQDLKSIKNKMLNSNRCYNLTSRYKSSLDIQLPKVNSQKRINLQLPSISPYQTRQSSFIFRQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XUB7</accession>
    <name>A0A8S1XUB7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">V-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPENT_87.1.T1390082</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000139">
        <property type="protein sequence ID" value="CAD8204991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XUB7"/>
    <dbReference type="OrthoDB" id="27923at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15843">
        <property type="entry name" value="R-SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01721">
        <property type="entry name" value="Sm_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000002">
        <property type="entry name" value="Small nuclear ribonucleoprotein Sm D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000222">
        <property type="entry name" value="Synaptobrevin 9-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027141">
        <property type="entry name" value="LSm4/Sm_D1/D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034099">
        <property type="entry name" value="SmD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23338">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="339"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Sm" evidence="14">
        <location>
            <begin position="5"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Longin" evidence="12">
        <location>
            <begin position="147"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="13">
        <location>
            <begin position="273"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8204991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="364" mass="41866" checksum="A48E2A155239F239" modified="2022-10-12" version="1">MSLGVPLKIMHEAVHHIVTVELKTGEMFTGYMAEAEDTMNVRLDEVQMVTRDGRPMSLEQVYLRGSQIRFVVIPDVFKYAPMFKKIRANAKSKNMQQIREKARQVRGIGTSYQIGFGIVEEMINKYFIVILNLNNKFFMPNSLKYLVIYRHETEKLIASYLIEAGSEEPLKSESTKVCFELKRNQLRIDERQKIDSANGSWFCKIDDKGLFYLVLGISTYPERHAYTLIQEIQNEFNRLGNDSLKDDASLKLHIKKPLRELGLKYNDLVSLDKIYQAQQNVDQTKIVMEDNIKQMINNGQQLDVLSIKAEDLNKNAKSFAKNSAELASIMYWRNMKLKIIIGLIILAGLLYIIVPIIIKVSASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XUI6</accession>
    <name>A0A8S1XUI6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1390096</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000139">
        <property type="protein sequence ID" value="CAD8205019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XUI6"/>
    <dbReference type="OrthoDB" id="308599at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035720123" description="Transmembrane protein" evidence="3">
        <location>
            <begin position="17"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8205019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="241" mass="28870" checksum="35B0C623ED42EB7F" modified="2022-10-12" version="1">MWKLLLIALTIIQLRAQEDELSEEDQESQFSQQDSQQNYYQQQQYGCIILTKYHLQERSQELMQLFKSISNDLDKRNAYEKLQVQLVMQCISKIQYQQLADLFNQLRQQTFKYQDFQDVFMMADLNLEDDYTLTDQEKILSKEIEQFDKNMQEQQKKYKQEQGYDDDDDDEEVDPRRRQQPQGYKLFGVDLSKLSNTTQMIIFVGLISLFSVILFLGLRFVQQHTQGPVKRVKKDKKVKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XWV3</accession>
    <name>A0A8S1XWV3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zinc finger C3HC4 RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1420113</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000142">
        <property type="protein sequence ID" value="CAD8206001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XWV3"/>
    <dbReference type="OrthoDB" id="300249at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger C3HC4 RING-type" evidence="5">
        <location>
            <begin position="15"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="220"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8206001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="434" mass="51565" checksum="C496F51DD8F7B44C" modified="2022-10-12" version="1">MNETQDSDTNFEVACSQCQTTPDNYVKLDCDHKFCLVCLAYNYLQSQQQNPESQDIVKIAICSKCQYETHLDPDTIEAIQYVIKEIIIPLIEQNQEEHNLDNDFDLKQSETIRESKANFVNDIVNEKQIEKFEQKDKYANNLQFQKVDQTKEVSEIKDIKKIKESISNKNEITPALNERVQLYLERLDKSFKNKFESIRDIQDQKFQFQQKCQSTRDEISQEFQKQILALEQKKNSLINEVNALETQQLLEIQKQENEYETQIQLMAKYQEELKNLKGFPEELSKFLIEIEKIMTAPSQSLQIKSLLLSSQRQLIRIQSLSQQTEERLTSDSKMITFQQKFSPVKNDRTNEIWNKLNESDKKSRLKESSISQQRSNQKSRWKETILEIFDKKEQSFERYSNNKYNPLNRTYNIHQVLNMQQQSFQIQKENLLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y091</accession>
    <name>A0A8S1Y091_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1440128</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000144">
        <property type="protein sequence ID" value="CAD8206627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y091"/>
    <dbReference type="OrthoDB" id="63533at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001773">
        <property type="entry name" value="Small GTP-binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8206627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="197" mass="22737" checksum="446619164300047A" modified="2022-10-12" version="1">MKEMSLTFKIILLGDSNVGKTSILKRYSENTFTEQQPPTIGLAFYKKVVERKNIKITLEIWDTAGQEKFKKIAPIYYRNAQAVLICFDVSKSDTLEGAKRWLEEIDKYLSSDCVKFLVGNKQDQGDFEVDQTFLENNHMKYIQTSAKTGYNVDKLFRRVARTLAKTKIKKMESLDQKTIITLQVQGPEEKQKKSLCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y2K7</accession>
    <name>A0A8S1Y2K7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1420043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000142">
        <property type="protein sequence ID" value="CAD8205874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y2K7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8205874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="21412" checksum="00F6436158B30289" modified="2022-10-12" version="1">MHKHQLRLIVSYLFEQYFRCGQLFYFVLMICFKTKIQLNHKQGSFVKIQLMIQKRVQLSNFISSNGYHRRFRSSDVELVQKWIQQFFWLFEYFLIFIMRISFGYGLVIEGGIHGSFEDLVEYINDTQQSQEKSGSIDCICISDNLQDNKEIEEHEAPIYKLKWILEITKVFIKIWGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y2Q6</accession>
    <name>A0A8S1Y2Q6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1420078</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000142">
        <property type="protein sequence ID" value="CAD8205944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y2Q6"/>
    <dbReference type="OrthoDB" id="288375at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8205944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="186" mass="22775" checksum="C0E4269EB6CE7A59" modified="2022-10-12" version="1">MQFFTPKFSFVVHKTFKQKLLARKEKRRFRGLNVYVPEFTGEGSIHPWLDAKRIKLLTKFYEDHRNKHRFTFKLSSDDKKKLNEVMQNYAEIHYLRMLQEKYWLDKHTEVIMNVQKEVNSLPYVLKSELDRKLSEKEMEYYDRPQLEPDSVYFEQRLRTLPEEEALNFEFAQRLFRIAQDKLAQNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y5I0</accession>
    <name>A0A8S1Y5I0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1540035</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000154">
        <property type="protein sequence ID" value="CAD8209285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y5I0"/>
    <dbReference type="OrthoDB" id="448774at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006626">
        <property type="term" value="P:protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051982">
        <property type="entry name" value="CiliaryAsmbly_MitoImport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45984">
        <property type="entry name" value="RNA (RNA) POLYMERASE II ASSOCIATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45984:SF1">
        <property type="entry name" value="SPAG1 AXONEMAL DYNEIN ASSEMBLY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="127"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="161"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="395"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="604"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="395"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="604"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="630"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8209285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="784" mass="91552" checksum="6F31AC20243BFC70" modified="2022-10-12" version="1">MISDDLTLEKIDFEWVNSQTKVAPLKKALKLLELDGNYYTDLIKAVEEKLVSIDPKYAKASEVVIDPVVKKQAQIDVEKWAENPTLEKNKRLAEQEKIKGNEALKSNDLKEAINYYTQSIQFDRQMAASYCNRALVYLKLKEYQNVINDCDYAIALQPDYTKAYHRRAKAYFALKQYDKAYLDFKYILQEEPDNNEVNGELRECRKYLTEQQINSAENKQFIEKSGGFKKVQIVEEDEEEEEIPVSKQKQPDFSKEYNEIMNKKNEITNRIQKGAYSQSIIELIELLKENQKYIEQHQFVELRAILLSNLAYCHLQLQEYSKVIEYCGNILEDNIAWDIKTKAYLRRGMAYERLDKVVLSKLDFLRVKDLDPGNLQASQALDRLSKIMSQEENNKVSKIREEEQNKQEIGVQSKKVEEPIQRKKEELHNNQIQQPVNTKKSIQEVDDGQKLQLSDAEISIQLGKMKEQGNIHYKAQELEKAIQIWGEGIKFFEANPAPSQIQLYFQLLTNSCLCLSQLNSHKQVIELASRVLIKDQNNQKALYRRGVAYSTLAQTSQKLEEQNELFENGRKDLERLVYLDSENKQAKDKLLEVQKQHADCRVKLREKQESQPKQDKKDEQNKQQQEKSEQQQQTKNQQNKQKSVNQQQIDQIGQNLTVNVIQDLLNKKQLPETATIFEKNMNTFKKATSSQIIDYVFQVDNLQQLYSTKDLPTDILFLIINAGLALKQDNSYNEKLITILRDRLPKTHKFNLCVNMISKKEKALIKELLECHSLNDLFNIYQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y881</accession>
    <name>A0A8S1Y881_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1560015</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000156">
        <property type="protein sequence ID" value="CAD8209750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y881"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8209750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="109" mass="13193" checksum="C01136E1A061DF46" modified="2022-10-12" version="1">MRSWIANLRQRKSSLRCNNEGFQIIKRRCKQFIQGIILFKDKNKKSKENLTQMMDSKKRKSEVTNRFQPEREVIEKEAELQFNYNQLLKKYNFQKDSKKLVTDLSCIIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y9C0</accession>
    <name>A0A8S1Y9C0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1570056</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000157">
        <property type="protein sequence ID" value="CAD8209993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y9C0"/>
    <dbReference type="OrthoDB" id="10348527at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8209993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="267" mass="31520" checksum="EA3EE99CF2CA11D3" modified="2022-10-12" version="1">MGSVSQSNRVIITHQEVQRQIFGIHQQNYGNQIIQDNFDNQYNQNNQSDEDIQFYQDNQDNEDEDEEESQENDTSFNNLIIQFKLIDEFIQLHQQEVQRRKQEELLWKQFKMIEQLVQAHCDYVKLNNFLEDLEKSQKQNNNSDQIELNISLFYQKKYSQSIDYPCKHAKCQTVATMNSKGEVNCKKGCPSFTIKDALFDITYNKVFNKIENINIVNCVSIQTSIEMAQILKDSMARSQMNETDKNEFCKNFSSVLCLQFINLKKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YB28</accession>
    <name>A0A8S1YB28_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cilia- and flagella-associated protein 418</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1590042</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="4">May be involved in photoreceptor outer segment disk morphogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Photoreceptor inner segment</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000159">
        <property type="protein sequence ID" value="CAD8210398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YB28"/>
    <dbReference type="OrthoDB" id="259905at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029239">
        <property type="entry name" value="CFAP418"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33958:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33958">
        <property type="entry name" value="PROTEIN C8ORF37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14996">
        <property type="entry name" value="RMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="30"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00026215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8210398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="142" mass="16723" checksum="352FFC1335B0E303" modified="2022-10-12" version="1">MINNKFDDDLDELINDINSVMDKPQNKPITSQNYQQQPQTNQSQQQKRKCLQPQIGNQQQFRMCTNLRCLKCDLQVKCHINQKWNKDVDYLIFRNYFNNMSILSKYLIDDNQYNAYNCQCTNVNAHESIAAPQNWVCSGHAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YB79</accession>
    <name>A0A8S1YB79_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA primase large subunit C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T1610039</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic-type primase large subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000161">
        <property type="protein sequence ID" value="CAD8210871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YB79"/>
    <dbReference type="OrthoDB" id="421393at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005658">
        <property type="term" value="C:alpha DNA polymerase:primase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07322">
        <property type="entry name" value="PriL_PriS_Eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058560">
        <property type="entry name" value="DNA_primase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016558">
        <property type="entry name" value="DNA_primase_lsu_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007238">
        <property type="entry name" value="DNA_primase_lsu_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10537">
        <property type="entry name" value="DNA PRIMASE LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10537:SF3">
        <property type="entry name" value="DNA PRIMASE LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04104">
        <property type="entry name" value="DNA_primase_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26466">
        <property type="entry name" value="DNA_primase_lrg_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0639">Primosome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA primase large subunit C-terminal" evidence="10">
        <location>
            <begin position="299"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8210871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="487" mass="58512" checksum="82FC4E9F1D6624F7" modified="2022-10-12" version="1">MQKLILEYEAILYIEPYLDQQTEEINKRDLLKMCVDRIKAYQRVNQLYSQFEETDQLKDQSKLIDLYSKAIKQYTEYGCYKGNDKFWIEDNAAHFFLELAYSDSDERINEFIRYQKQLFQIRCEEQLKNDETNDRLIKIYKQLLGDDQISNISKQEWEEDVPKQLRLFNQVDILGKHVILGTGKSQLRIFNNANIYQKFFKTPFQNVLYSVSSLHTYIRNGFAYIHVDELKNCLLDLFLGRMKKRMQSHQNKYQYEELKKDKDLMDMFMKIQQTDSGMLSQKYQNFEQDSLNALTVRQQAKDHFPLCMEYLIDRLESNHHLRHGGRQQIQLFFKSAGMKISETVKYMKMQFQQKISEQEFDKQYSYNIRHNYGLEGKRENYQCYSCTYNMKQNPGNDEYHGCPIKNFNSDVLEKYLKSKSYEETDIKRLIELKKGNHFQLACTEIWHLKNKESSNTLIIDNPIVFYKESLNHHKKIQLKVETEEPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YD48</accession>
    <name>A0A8S1YD48_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1680015</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000168">
        <property type="protein sequence ID" value="CAD8212396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YD48"/>
    <dbReference type="OrthoDB" id="282672at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070224">
        <property type="term" value="F:sulfide:quinone oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070221">
        <property type="term" value="P:sulfide oxidation, using sulfide:quinone oxidoreductase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000593">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015904">
        <property type="entry name" value="Sulphide_quinone_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10632">
        <property type="entry name" value="SULFIDE:QUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10632:SF2">
        <property type="entry name" value="SULFIDE:QUINONE OXIDOREDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8212396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="472" mass="54630" checksum="1C63B62278971B96" modified="2022-10-12" version="1">MLISGMQLRHSLTPRYFAGVFNKRKPTKKDLDHYDVVIIGCNLGGVLSRQLDKVSHGKYKTMVVLDQNINQITPIRQIYEQQKTAKTDFLLNAKLSLNMYTAHSDQVGCQTILPEENAIILRNGRRIGYGQLVVAMGQQVNYDAIKGFEEAWQDFDSPVFTNLDHPSWRSSNHKYTRWHYNFNHGEAYFCIPQFPFSGEVESYNFLLSQRIWEWQTANGRQSPIKRLTIVQPNERFVQYNDAGDQFFKEEIKKRNINVEYGLKLVEVNKQQNTAVFEDVKTGNRQTRPFNHLYAVAPTKPHDQLVKAGLTTQKGLLDVNIKTLQHNKYKNIFGLGDVNDVPTTNCFWAGFHQLHVVRNNIERNIAGKSLNAEYDGYSKVPILLGQNTLTFLCHKYNNENAWHNLYFSNGGFLAALRYYNWCKNFKKAFIDIYLEKNWGPPYYKIKKSFKLPEGESNDHGFLAKLLPGKKEAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YG33</accession>
    <name>A0A8S1YG33_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1590048</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000159">
        <property type="protein sequence ID" value="CAD8210404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YG33"/>
    <dbReference type="OrthoDB" id="290744at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8210404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="181" mass="21245" checksum="6F5DBA0E0BE1E961" modified="2022-10-12" version="1">MLIDNQLLHNHNHVQIKQKRQAPKTERLSLRLTNVSPFRSFHQTERDQLEQLIKQSIQNPKNVNTLLNEFLSRKQEESRARVFSQPQIQAGECSPYKRIIKQKAASRPNSKEKRIDTTPISPNSSLPFKKQVTLHIESTYSQKKQTTISKLSTNDLIQYHKQLIQRAQKMLLYAQFKRSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YHM2</accession>
    <name>A0A8S1YHM2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1760002</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000176">
        <property type="protein sequence ID" value="CAD8213239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YHM2"/>
    <dbReference type="OrthoDB" id="325447at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8213239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="22142" checksum="B5E15A1E6E0BE1D6" modified="2022-10-12" version="1">MKYLVLTFYLSRLIIFSLRLSRFSTKSLVKKEFNYEFIIICIIYILINFILAFTLLKNFGGLQYELKLNFKGFITITFYILYGPEFSSDGLFIYTVEKNEPVSKSTKSYNNLYSNGEKYRRVMKVQIIIKIHQQQVRNASDQIKAYCGFKNYYVAVHKCQPYCLQCLNETNGVAIMVQMLLNFHKKSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YJ86</accession>
    <name>A0A8S1YJ86_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1810009</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000181">
        <property type="protein sequence ID" value="CAD8213568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YJ86"/>
    <dbReference type="OrthoDB" id="295282at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="513" mass="61049" checksum="FAD26742B948043D" modified="2022-10-12" version="1">MNLFYIVVSISLEFTECQNKNELMFKIMEQTGHHYYSMIIKREQFQQNQISEKTFNQVSVLLTVMCKECLELEEQYIDYFQKVIQHLLPDNRNNQNLFFFHFMMGAFSEIYLILQQVELCIQKGLIPEDKNGNAFSICFTLALIDDQLLPNNLNAKALQIIGRQIEMWFQQSQQNTLKILQSLITKEKFLDESLQFKELMQQTQTKSIEDFCSILFKSLEALQQVVKFKNENLFKKTINSKIEHILYTAILMYLEQMEQNLEQKTKLISYWFPAVIKNKQSIPLDQKQLAQQQIHLHLNDQLKLEALDHLTSDFNTKGKVICKSISKIIINEIISPNLFTPLHQDLKSMTDAKSYQQKSEWSMLQQLDEIKDFFLIFNQKLPVNYHSIFYDSFIASFFEGLFYFIFDINKLQNISQDLYFELLTLEFQTLQSFLEKQISSNVLQMRIKQQLAEIKDISIWFSMQNKDLIATFEQYHLRQDNKKICSALYRLILLRKQDSKMKDFAKKYQKIYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YJN9</accession>
    <name>A0A8S1YJN9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T2190005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000219">
        <property type="protein sequence ID" value="CAD8214495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YJN9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="234"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="279"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8214495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="498" mass="58008" checksum="0E5508A6F4558471" modified="2022-10-12" version="1">MLMRCTQADHLNQQIIGVCIDSKCQYQRPYCNSCLPIHGQHLNKLTSLELLDEWISKRVLIVQNVEQIAQACKLEIDSLLNKFVNLYNINMVQLPKLGLSQIDNIIKGLSLVELWEKTLLNQFQQSIQQIKQIIKEILENQQLQTNLSSTYNKQVSKSFVIKEQICEQRINLNLIKPNLKPFTFEVMNQNSIKQEEKCFAIAINKDNSILLAGCDRYIKVFDFKQGKLNQLQLLSDHLNWVSTLNFMKKSNHFVSGSGDKSIIIWQMNDNNYWICQHKLKGHFDDITTIQLNNNENLIISGSYDSSINFWNKQNGWLCQQTIKDHTDYVYQLSLNEKEDKLISCSADSLMLVIEYSQKDQIWTVIQKIKVEQYGYRLSFLDDNQFAFQPMNQQQMYLYERISSNQQFIKTKQISVKSASKNCANLFPQQYLKSKCLIVSKNGQNINLIQKKENGDFINKQNILIGHNQIFGSLSDDGEYLITWDGKSKEIKIRKCHQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YL05</accession>
    <name>A0A8S1YL05_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1740029</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000174">
        <property type="protein sequence ID" value="CAD8213137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YL05"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="53" mass="6476" checksum="DA33B9448D4D3F4B" modified="2022-10-12" version="1">MNSQTKLNAFHSLRGTFAKNLDYSFEYPRMVHDRLIYLHQLRNFHEFNLSKVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YLN3</accession>
    <name>A0A8S1YLN3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T2140006</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000214">
        <property type="protein sequence ID" value="CAD8214438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YLN3"/>
    <dbReference type="OrthoDB" id="320626at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8214438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="246" mass="28760" checksum="1F6F767FEF1DD5B4" modified="2022-10-12" version="1">MKQPALGGGQYDEAGNECKIGRWIELSDKFKATCQVRQYGEYINGKKVGRWDSYWHYEKLYKHIGGGLYDESGNECKIGRWIEITEEFNSTSQVSYHGDYKCGKKVGRWDIWYQKNYHDLKYEKIGGGSYDEVGNGCKIGKWTEVIHDFSDFSQVTYLGEYKNGKKIGLWEIQYEAKKIGSGSYDEQGDMIKIGKWIELHDPFTDSFQIIYEGEFKRGQKVGTWIQKERYENHLDFKEVDVIKYAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YP93</accession>
    <name>A0A8S1YP93_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1730029</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000173">
        <property type="protein sequence ID" value="CAD8213064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YP93"/>
    <dbReference type="OrthoDB" id="308556at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="33"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8213064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="253" mass="29459" checksum="701281633F9785FA" modified="2022-10-12" version="1">MNKSNHFISGSQDNSIIIWSMNQNNQWINQQKLNGHCGTIFCLILNNNEDLIISGSSDKTIKFWIKQNQQWLCQQTITDHTSSVYGLSINQQQNKVISCGCDGQILIIEQSQQDSKWIVIQKIKVETYGRRICFINDNVFIFQPNCKEQMHVYEMNNTNKQYLKTKDILVKGGVSDDYCLFPQQHIKSKCLVVNKNGKYVNLIRTKENGEFKTEQSIEFGHQNLFGCMSDDGQYLITWDYSSKEIQIRKYNEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YQY1</accession>
    <name>A0A8S1YQY1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1930007</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000193">
        <property type="protein sequence ID" value="CAD8214004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YQY1"/>
    <dbReference type="OrthoDB" id="2104158at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8214004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="173" mass="19941" checksum="4F98F1BECE970D79" modified="2022-10-12" version="1">MAIFGKISANVLNLSLTCNDLNTGIIGNQLKIRATVTMNGLKRNEKNCLLLQPIMVLRRHHQVVENFKLPIELQLNFLICQNHSSKKNHYKNSVFTQSINYNSQISKQYWDANLCILSAKFKYVILKQKQKKDAKKKGKPFYADEPAQPPPTLIREIYEVNVTVQDHLAEFQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YRH3</accession>
    <name>A0A8S1YRH3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T2090004</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000209">
        <property type="protein sequence ID" value="CAD8214364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YRH3"/>
    <dbReference type="OrthoDB" id="10430173at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8214364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="131" mass="15067" checksum="501E5ADB21BE478D" modified="2022-10-12" version="1">MPGLHPYQCVSYALICKGVTEVLVKIEVNGTQHQCHPRFQLWGAGSPTRDEYIVHCGFQQGVSLVMEQLPDQNYKYYCQYFADGSHNKCLITAYKFVNVHLWDMVSVVIKLNGNTMKDLQVPMKYVVMEIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RVH4</accession>
    <name>A0A8S1RVH4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0010385</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RVH4"/>
    <dbReference type="OrthoDB" id="293577at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8131986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="609" mass="72598" checksum="3D20AA76B64C18B5" modified="2022-10-12" version="1">MIIKYNELRVSITPKTQTSLNSPVRNNTQPVFITSPKKQLSSIKEITNKQQELNKLLNQHVDFQTQSANTSINSFDCITAQSKFRRKQINADISSKLMYLDDEIVEVYNEFEKNYRQINDLDKLEYTKQASQKQIHVSRKSLNNLIQTHDGLMQSDLKFGNMNRIRINRFKFYYFRIRLRGKVSPIQVFFNIPERVQSSAVKMFLSTKAEFPTKFNAEYILHSRFAKIFSERNQHYFTEEYLFITIYSDVDFEFSITFQFGNTQITKSPPKQIIESLDLIQTNSSPIQKQLPNDKILQNLSLTQYHGSQKLNKILSVQSERTHKQKMAKSLRQSILEDKQLDKKSKLLVKDQILHFREVERQIKSKKLQMQFAQQNWFQLFSLLLITEYVSNSLQEQKRKQKVAAKGKLLVWAMKTKALLDVKEYGESAKERTIFKTRCVIQSFASMIREKSKIKAEFVITKFMGRILLFLTILNKHQSTLNKVIFIQRKFRLLKAKKRKFRDKFWKLIKENIADIIYDLRRQKETQFFFDKQQINIDIPQMNIVIDEYCKKQRILWMEYIQHTFLEKDQKRKIHEYAINLKEPKIYDLPNKNELIPLIEQYAKMKKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RX48</accession>
    <name>A0A8S1RX48_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0010082</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RX48"/>
    <dbReference type="OrthoDB" id="295530at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8131485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="83" mass="10021" checksum="536DEA1C88740B81" modified="2022-10-12" version="1">MPNTSLVIINHQKRSVQFSQQHTVHIVENWKEFNYTPKEIKNKDKQLKVEKIVIRTDFENYMLEKYNKIKSTLKEFTNSCSFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RZS5</accession>
    <name>A0A8S1RZS5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0020430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="285170at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002895">
        <property type="entry name" value="Paramecium_SA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01508">
        <property type="entry name" value="Paramecium_SA"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SMART" id="SM00639">
        <property type="entry name" value="PSA"/>
        <property type="match status" value="17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035783991" evidence="1">
        <location>
            <begin position="20"/>
            <end position="1335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8133488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1335" mass="149148" checksum="CC346D00B6ABB4DF" modified="2022-10-12" version="1">MKNMMLLLLNLLLVSYSELLPKVDQCTCKQILSSKDCTQLTKCQWNTASGSCEDRKSTDLESYCSLNSSNCPINGCALQQGICKPFSGCSVYQGKTHEECQKISRLCTTNGEHCINIDLLCDNFNDNQLGCVVDLDGYPCYWNNDNKKCYQVQNCNELPSSYTTHQTCYEAGQIKMLKCTAKEGGGCMDITSDCASLQKLGCVQNQLGKDCFWDGSSCKDKTCTNAPNSYTTHEQCQKYLNTCSLNNDQKGCMDMPNSCDSYINESQCIQVQGTQCFWFSITCKDGDTNCQPKAECKSWTCENAYSTYNNDIQCRQFKSECTVNNTNNGCIKRLESCYEYTTQNQCVSVLNDQQNCYWNGSKCVDKTCINAVLNKYDQVSCSRYMSQCTVFNNKCTQKTCSTYLSEKQCSNDYQNNKCAWSGTCTLKTCENASDELTSHKDCQQWLNSCTVQSDLKGCQNLEQVCSAYKIQDQCYLAGNTKYTCLWINGQCVQKSCSTASLYIYTDEECNSYLSGCMISDQKSGCVSRKATCIELQEHQCSITNSGSYCFWNGLQCVARQCTQVVYNTFKACNTFLSTCTANYDGTQYNGCVNKQNKCNQYNNEFMCIESLSEGKCIWNKKATPNACEVRSCQNSDQTTSDEACNNFLNTCTVNSARTGCIERYSKCSEYANEINCKYTKSGGECIWYNNVCTDRTCDKADKTYTSHNECQSYSKNCTTNGKGCIKIDQCSQYTTKSGCIIDNNNQLCTFQPSCNLQQCSDAPQSYSTDEQCKKYKKECTTNGNGCVLRTECSDTYIEQACVTDSNGNKCTWINNKCVAYSCSSAPSKYVTEIECQLHKPGCTVNQSGGCTNKGLCKDAKIQQACTTDKYGNQCRFSKDGCRDIICSDVAYTNHYDCAKFNSQCTSNGLTCITQAKCDTKIQSGCFLGSDGPCLWVKNGCYQYSSCTSLQFQTHEQCYEFSNECTTDGFTCIPIDKCEKLPAQGCIQGTDGKCVYLSDKNKCVVFKTCNSIEYTTHQDCQNVNKTCTTDETKCIELQDCSSYTKQSNCQLNSDQQKCYYDEKEKKCLDLQCSHIQFTTHEECNTALKTCTTNTTKCISIDKCENYSKEYCNIALGSDGKCKYDPTDNKCRLIKCTELVDNCTQISKCVDSGIGCVEQFTCDKYETEKGCKQGGSDGYCVWYLDNGEGKCKIMSACSDASTNKEACQSKSWTCQWTETATKTTCAQHTCSSKSKETGLCLPILDFFQKNYELCALSSEQCISAQKSSLGAQTCFQSTAYTYTWDSANSFCLQCGTIYTNTNNQTNNSNNSNTNDTDTDNQVQILFPMLSLIISIYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S4W2</accession>
    <name>A0A8S1S4W2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0030409</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S4W2"/>
    <dbReference type="OrthoDB" id="312108at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8134642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="276" mass="32839" checksum="2D3B578C5FC088CE" modified="2022-10-12" version="1">MDQPQYSIHQILQNKRDLLRETIRNNDLEKIFQQKRIQTQQNQESEEISILSSSSSINYRKNQSIIEIESSSEEASLIRLTKIRIKANLTPNYLISVNEYLKRDPYIFQVQKIFTLCEDAILDIKLTSSDLVPYLVEIIYSVAKYKTQIPFKPLTLLLQAFTLHQDYIILQYIKMYLQNNKQETSEIDQFCKQKSLFQDESFLFIICNNIKYANLQMLLFLCETIKNNDFIQAIQVYGVKIFDKNIEYKKEYRKIIKEIQILAEIRLSNKEILYQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S7K3</accession>
    <name>A0A8S1S7K3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0050042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S7K3"/>
    <dbReference type="OrthoDB" id="5590282at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000281">
        <property type="entry name" value="Cyclin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8136226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="187" mass="22792" checksum="95478BA2E2A56BC3" modified="2022-10-12" version="1">MEKEICKILQWHLNIPTINLWIEFYTNQWDNYISDVHKKFRQNNNCSFKMILKLQGYIDCCYLDFETLSYQTRKVVASFMYLVLALEYEQFTKEYIYYEIPKTTKFILADSQKSFNKLFTEFAQISFGFNLVDLIEIIKYASKFIMLDFKYSDTFQDKISIQRHEDLLIYQKYNQTGLSFIRYRLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SCU0</accession>
    <name>A0A8S1SCU0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0050393</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SCU0"/>
    <dbReference type="OrthoDB" id="290665at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF110">
        <property type="entry name" value="DEVELOPMENTALLY-REGULATED PROTEIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="383"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="9">
        <location>
            <begin position="642"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="86"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="209"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="92"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="210"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="234"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="412"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8136882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="875" mass="101566" checksum="AFFA4EAFFB787EC3" modified="2022-10-12" version="1">MKKLNQQMQKIAGRNSFQESPQRLEKKTIKNPAKTVKTERPQQKGKTKLQDYFKKERNSLAQVVNNIRAKLKGSKSIDNMFLQTQKSERTKTSQQNQIEQRVQTDEGNSSKRLNLKTKNLSLATVSIDKKEYQKQPSTTKQSYNQSPSNQSKPILNKSKSKDQYLFDLLKNAQMISNQYQQYFNLKQNKQSIQSDTSTNHRQSIEDMLISQKSTKGSPQPYSSKLSKKTEGDSKSLNQGYSKTEQTPIIKQQSKTKIEEQLQYDTKKQLQIVLFYKQTKYYYLFDYINQKTDDLYNFLIEQIASIEVISAKIEGGGTGSTEDQYIQDELNKICQFITVTKNIPYDYYLSLPDLPLNVFQGKILQLQPLYDQPKETQRVGLKDFNLIKCIGVGGFSRVYLVKKKDNGQFYALKLIDKKFIFDNAKEIIVQNERDIMVRMENQYIIKLHYAFETRFYIAFVLEYSAGGELFYHLRKLKRLNEQEAQYYFVEICIGMAYLHSQNIVYRDIKPENILLDLQGHMMLSDFGLSKPNMEDGELAYSFCGSPEYMAPEMLMKSGHSYLVDCYCLGALLYELVSGLPPFYSHDTSEIYNAILTENVQFPDYVQISDQLKDLITKLLQKDPNKRLGSNGGISEILTHVWFHNVDFEGIVNQTLPTPYKPEPLKYNFDEEEFNKGDAEFRKQYAFNLQYEFQNVDNANYELENFYYARPQEELKNRTNLVNTSKLVSQIVQQEPISPNKQMISKVKHSPQESKNIQKPNIKSEAHDYFKKHNLLTKSSQILQQSLRLGHAYSKTLQQPSMSIQDLKKLKSLFDQSKQLLSSDRVTTIPDQNNKQILERLKTEQFGNLPSPKTTTNSAANKLAKFSKLFGSEKRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SES4</accession>
    <name>A0A8S1SES4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alpha-1,3/1,6-mannosyltransferase ALG2</fullName>
            <ecNumber evidence="3">2.4.1.132</ecNumber>
            <ecNumber evidence="3">2.4.1.257</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">GDP-Man:Man(1)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0060169</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Mannosylates Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + GDP-alpha-D-mannose = an alpha-D-Man-(1-&gt;3)-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29515"/>
            <dbReference type="Rhea" id="RHEA-COMP:19511"/>
            <dbReference type="Rhea" id="RHEA-COMP:19513"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58472"/>
            <dbReference type="ChEBI" id="CHEBI:132510"/>
            <dbReference type="EC" id="2.4.1.132"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:29516"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>an alpha-D-Man-(1-&gt;3)-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + GDP-alpha-D-mannose = an alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29519"/>
            <dbReference type="Rhea" id="RHEA-COMP:19513"/>
            <dbReference type="Rhea" id="RHEA-COMP:19515"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:132510"/>
            <dbReference type="ChEBI" id="CHEBI:132511"/>
            <dbReference type="EC" id="2.4.1.257"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:29520"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase group 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.132" evidence="3"/>
    <dbReference type="EC" id="2.4.1.257" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SES4"/>
    <dbReference type="OrthoDB" id="448893at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004378">
        <property type="term" value="F:GDP-Man:Man(1)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102704">
        <property type="term" value="F:GDP-Man:Man(2)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027054">
        <property type="entry name" value="ALG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45918">
        <property type="entry name" value="ALPHA-1,3/1,6-MANNOSYLTRANSFERASE ALG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45918:SF1">
        <property type="entry name" value="ALPHA-1,3_1,6-MANNOSYLTRANSFERASE ALG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="33"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="4">
        <location>
            <begin position="159"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8137592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="337" mass="40052" checksum="CA949DADE4C70499" modified="2022-10-12" version="1">MKKQDVILYQLIFLVMIQVMKRKFYSNMCLLRMILATLCKYFLVVLGMLSLLIKYLSFFLCFGYQEKDLYFIVIFLQIIIFREKIIYQKNLQIFLGFIEQISMLFANLVLVKKIVIQAFPFNNKYGKQPQVLYSAISFSKFESSPYLNRHDSTLIRNSYFISLNRYERKKNIALAIHVFAFFFRQSLNQDENIQNIRLVVARGYEQRVEENAQPLINQIRILKDKILLNILLLKKTFQILKEHGLCQMHQPQYIPEREHFGIVSLEAMYNEIPVIACNSGGPKELIQNGVTGYLCDSKINEWCQKMIEIPQNKKMNKKWENEKGKEQFNFLDLYNFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SFI5</accession>
    <name>A0A8S1SFI5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0070194</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SFI5"/>
    <dbReference type="OrthoDB" id="298839at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="1">
        <location>
            <begin position="92"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8138596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="214" mass="25756" checksum="6EC46CF4DC78AC88" modified="2022-10-12" version="1">MSNTNKQHDVCIDENKENLTIQIILSEQESQEQALSNYSTQNDQQNLVNFDYQEDISAIVEHFKVSIKKGQWKDQISKNIHKTRLKKTQYKKKVKRIKEFQLEEDIKLLNLVHQHGRQFSRIVKQFPKRTVSMLKNRYYKNLRYRWEELMGIEYTQNEYESKTENQVNRKSGDNNFVKHQDLINMIPDSYSCPIICDMLSTFITKMDQFLKFQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SG10</accession>
    <name>A0A8S1SG10_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0080032</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SG10"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8139305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="327" mass="38682" checksum="3786198142452433" modified="2022-10-12" version="1">MISIVPILQKFIQEGQQYFKAYNDLEKNKNELAQVISEVTILLSEIQYFERYEGFYNYDEKQLIQQCKDKLHLSDTVLKQSPSKVNIILDYFYITGNNYKELIDCQNVLNGYKIQIDELLRKSQEIFVTVINPINQQPQNSYIYNLQIKLKLKAPRSIKSIFDKYPYHLKNAYLFQFSMGDSCASKVDVHVFKRDEFTQLYAIEKQFKTYTISRRNHCTLQMRNQLQFSPQKKVCSNSQMETPTPEDYSIIIPQKDFNSLSNKFKLYLIVNGQNGMKIIRNSKEILHIRSGQECELQEDDQIVFFQDCNENPQVSYIVQQILIKQEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SI95</accession>
    <name>A0A8S1SI95_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0070137</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SI95"/>
    <dbReference type="OrthoDB" id="286327at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8138482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="378" mass="44319" checksum="1967781FC6F886FD" modified="2022-10-12" version="1">MLFRSAFRFCSFNKDLFQRQKPLIQIDNIIKILRPFDIEINIEEMVETVKIVGKYSKHTLYKFPIPSYPQFISETQQRIMKNIPSNPKNLSIFYRMYRYPMRMALPKTVFQISKSLGKSSLSEQTLRLAACLENIQIALSLHMMIDRYHTQKPSIFSLERIIYNKLSDKKLVLGGDFFHSKASVLSANIMKQVEVPLFLSMLEEEAAKGVFYEYDLFEKIKEKGASTIVPEEVYQLAYHQCASKFAFTTILTYWLQNGDLNKLNDAFKAGVYLGLCIDFQNQMKYFVRNLPTMSNTLLKAVVNNNECNFYQDKIALPIYYMHQKSKETTSLQQLHDHALKQSEKVIEIMKDKSKEYFLKLGANFEDIEDLIEHFCRYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SL72</accession>
    <name>A0A8S1SL72_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0110018</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SL72"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8142211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="59" mass="7124" checksum="AA77BFF25E641D3D" modified="2022-10-12" version="1">MKEMRRRLNQIISSSFKMKKISWRGNGTLKKNTCLNMNYSNYQEYSYVTKTNQQLHSLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SLA7</accession>
    <name>A0A8S1SLA7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0070503</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8139164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SLA7"/>
    <dbReference type="OrthoDB" id="434160at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11732">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSP105-110-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000004">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639:SF4">
        <property type="entry name" value="HSC70CB, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639">
        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="527"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="772"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="774"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="787"/>
            <end position="796"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8139164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="802" mass="92569" checksum="DE852F46695DCF27" modified="2022-10-12" version="1">MSQLASIGIDFGSQRSVIAAALRGGVKILDNEGAHRETQNVIGFTAEERFIGEQGALQQKSNFKNSIGFFNRFLGIQGKQAFRAEEQKWLTVPTSIHESGKTLFEVTYLGQKANFTPEQVTGTMLNKLKNIIQLNDINIQSSNVCISVPAYYTESERKALIDACKIADIPLERLLNETTAIAINYGLFRKTDLDPEKPRNVAFVDLGHSKFSAFVGSFYKEKAQVLAQVCERNLGARDIDWALFEKFATQFEKQTNGLSVRKNAKGRLRLLEAIEKARKVLSANSEAQIHVEYLVEDEDFNTLIKRDEFEQLAQPVLLQIQTQLELLYNQVQNLKLQLHSVEIVGGATRIPAVQRLIEKIFKIEQVSRTLNASESISRGCAMMAAMRSPNFRVTEYKIEDCNYYPIRVGWLYDQNLNKLDQSKGLVKCFPEKQCKILFEQNNPIPCIKSFALMKTQPIQITLFYDPVPQGFDPIIQQIQIPPLVAKHKEHSTKIKILLNQNGLLNLEEVVFLEEFMEDVKVPVEKQKTTIPQKQDEGQQQQQDTQIKEEPKVEETQYEIKQKKKTIQNQVNCEVSSFYSLTKKEIDYYFQQECEMLNTDNQVHETQFKKNQLESYIYAWRENLNGKYAENATPELKDKIFKELDLQYEWLYGEGQKNTKRQYSDRYDKLVQLCGPIATIADEYRQIPEVIQQVLQYCLIYENFVNSQDLQYAHIQQEEKILVANELKTFKQWCNNTLDLFSKADKTVKFAISVQQINAKYAELQHKCQPIVTKPKPEPIKEEKIINDTKQQQQNNDNKMETE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SLB3</accession>
    <name>A0A8S1SLB3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">COPI associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0100125</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SLB3"/>
    <dbReference type="OrthoDB" id="423534at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013714">
        <property type="entry name" value="Golgi_TVP15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28128:SF3">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_46, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28128">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08507">
        <property type="entry name" value="COPI_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="131"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="132"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8141455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="158" mass="17856" checksum="176E8CA5F2E20C8F" modified="2022-10-12" version="1">MKCDIHCATKILTIVCGGLIIALAISRFTNLSIEHPQQFILTIHYLWMGAVLILAEFRFAYIHQKFKILTSFFGRGLYCFFLGTLMISAFSPLQIATAVVLFVVGLIYILLQLFSKKFGWSTEEAKNPYQIPSQNQPNAYQPPYQSNVNFQQGGPALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SM40</accession>
    <name>A0A8S1SM40_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0090119</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SM40"/>
    <dbReference type="OrthoDB" id="301748at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8140526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="227" mass="27359" checksum="6B14B1F58BA43846" modified="2022-10-12" version="1">MDLMDNQFQQKFQQLLKLKEIYSTRNVLPERIQRLKLIDINFQIKYIRQKQQNRLNQIINSSIVSQNIPKNQQTERSFSNQLNHLNIADQQNICLSCEKYIFEKKVTLHCQNKLNHNYHSNCLANIMKQQLANQCIKFQCLCKSQISNGQISKQKILELDIYINRLMLNQLYYLKTHYQNIKQCANKDCQFFWIYKQQQKQIKSRSNSPVKTYKITYINYCPDCRFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SM69</accession>
    <name>A0A8S1SM69_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0090134</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Nth/MutY family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="313508at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004035">
        <property type="entry name" value="Endouclease-III_FeS-bd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00764">
        <property type="entry name" value="ENDONUCLEASE_III_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="195"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="452"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="553"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="602"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8140556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="824" mass="95160" checksum="0071FC335461B6CD" modified="2022-10-12" version="1">MVFQDIWDIHGRCLDYCIFGSTDRRKRYCKYYQPSYFLYLYQFNFQYSQLIRWDIIYDPRYLNHENKKNSPFFGIFKYYEGASTIVNTTYFVYPLGVYLYIYMCNATPINSGISIYLNDTYYSSIYYDGTQYIGNNLNVYYTSNTVLSGCQTAKYIQLYTNLYVEQGGFTFSIKGNFTSGNSGWFLPYILMSSAHCPPYCLKCEQDYTCSQCVTGYKIVSEGNCVRLCPKTSILQNNICIKYDQVTKCKHNPIILDSKYFIQQFYDFTVPNNIKSSFVLESYTSKDFQKGDEIYWSYIETNVVYGGKYVWATARFSQIYTIDSAYHSLTIYFDAVFGCNFDGVLGYLGYSVNNQTEQQVTMNSTISINVTTPSPTLSISFQCYGPSNNVLDQYCAISHYYIVVHYCSPYCLQCLDEDNCQIMDTFDASVVILNPSQCSSTQYYDDEYLKCEECPQECETCLNEFECLTCKYPYQIFITKCLLQCQINEYFDEQNQICDQCNPKCKQCKLKNECIHCEQSKFRYLFYDQCLCYDGYYDDNINIECQVCNVLCTKCFGPSNMDCHECIILDKVEKIGNTCNCVNGYYFDKSNQTCMQCHQKCSNCFLSSEDSCLTCNNFENRILNGLNCSCQTGYFELNDICVSCPNIEDSNLYKCYKQCGDNSIIWFNQVCQPFSCLTGFTNQNNQCIPICGDLIKNGDEECDDGNNILDDGCYNCRYQCPKQCLTCNEFTILPCLNVCGDGIISGTEECDDGNLIQFDGCYECKLECQTQCTQCMRGLCYQCQTYGWIVDIDTKTCVENCGDSIVVGQEQCDDGQXIFWNFQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SSN7</accession>
    <name>A0A8S1SSN7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Lysophospholipid acyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0100163</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SSN7"/>
    <dbReference type="OrthoDB" id="286734at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030258">
        <property type="term" value="P:lipid modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049941">
        <property type="entry name" value="LPLAT_7/PORCN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906:SF4">
        <property type="entry name" value="LYSOPHOSPHOLIPID ACYLTRANSFERASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906">
        <property type="entry name" value="PORCUPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="22"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="47"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="337"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="395"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="428"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8141514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="455" mass="53784" checksum="9364BD95C783C8C4" modified="2022-10-12" version="1">MDLIDTKVIELSSKTGIPDDQLKIFISLIFCTLFGFLFKLSIRGRQTRLIAGSVFGVLITYYIYRSQIINVLFQTFVVYIMVKVLGKKSPWPVFVESMIFVAVHHIYRQYTDYGGWKMDVTTILMMDTPKWTAFAFCVSDGLKTQLSKEQEIRKIVKLPSFFEYFSFIFFFAGCVLGPCYDYFDFDQFINEDKEYKNIPSTIKETLRLLKNTLICMGIFLFAEKYFPLHFVTTDEFGTYNVLYQTLWFNIMVAIQRTKYFGGWQLAEMSIASCGITYQGNGQFQKIKAVDVDWDLTYNMKDKVEKWNISIQTWLRRYVYTRIYTEEEMKKSAGKQNFAYQMTYVVSSLWHGFYIGYYFSFFQWAIMNNVNKAIFRVSCNTNYLKIFETNPLAKFIRWYVANTLFNVFGITFLLLSVDPILKFYSNIRYVPHILLYVTYAFFLITNFGQKSKKQKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SSX2</accession>
    <name>A0A8S1SSX2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0130347</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SSX2"/>
    <dbReference type="OrthoDB" id="287176at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120330">
        <property type="term" value="C:rixosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045227">
        <property type="entry name" value="WDR18/Ipi3/RID3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18763:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18763">
        <property type="entry name" value="WD-REPEAT PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="244"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8144681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="385" mass="44750" checksum="739154DDF93C6646" modified="2022-10-12" version="1">MAINTSTNLIAVQTQNAIKLIDPKTSSILQTIESDIKTICFSNQSILIFSSKNMLSCFDYQGLELWKQHIGGESSIVFAFEYHSIYGIIYANKDFRIYTQTQELKSTLDFVAKEINKVYHYQQYLLVVDDLQNNINLFLLDDIVLLKTKPFKVFTGNNSKINDFVLKNVLATISKDKELIIFDITTTQIIFQTQLDSEPLTLDIDLDYAFVGCVNKNIYQIKYNQQSINYTIQPTIQINKRKLLIGHLSEIKSVKLFFEDGHNFITSNSEDGQIFIWQVDGNSIILSRKILTLQAQKLFIIKRPYEYQQQSEEQTLFKSKRLLQTKPLKKAKYTSNQMMVCKKRIQKVKLSFNFTTQQPHNVIDPQINQIIEENQKLKQIIHQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ST49</accession>
    <name>A0A8S1ST49_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein L17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0100243</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ST49"/>
    <dbReference type="OrthoDB" id="286337at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000456">
        <property type="entry name" value="Ribosomal_bL17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14413:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL17M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14413">
        <property type="entry name" value="RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01196">
        <property type="entry name" value="Ribosomal_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="158"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8141662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="26822" checksum="F484904137F81201" modified="2022-10-12" version="1">MGYRLSNPKRFGLARTSKQRWHLWRTQMTQLVTHERIHTTWAKARALQFVAHKTFKLGQDASKNRSNAINRLMGILTTKFAIKKLINEIVPKFKDPKEQVFKVVETKRRKSDFARMGYIEFAKNEIAKYEESQYKQLQETGKIVDISKYNKTWLIEERDFIKEKLDQAQSRFDALQADPNTPSVEIKRANQDVNFFKRKLETVEKDLWIENKNPLDMRRYFRIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SVJ7</accession>
    <name>A0A8S1SVJ7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0110189</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SVJ7"/>
    <dbReference type="OrthoDB" id="310057at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="331"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8142492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="467" mass="57589" checksum="0389CD340D01FCB6" modified="2022-10-12" version="1">MENLEGIYKFTFRKKKNQYLPEVHYICKRDDEDYFFYQLEGSNYISLEILLKSYLSQIIRLSIGQCLILIKLIYEKVIEVYRKEAKTLPKIKLENIWIYTEKGFNAEIKPQKSMKIIFLNYQDENVSIEENLKQLQQINYQIKKYCCKTEFQIRAKDGDSHFIQQTMNIIKDMFYEIEQSLYENPTEIKNTIDKYVNNETQETDYEYFQEDVQNIKRNFEIVIRNCNEEFKKNCQNELQLHRKLLNWQKKLFDYFIENYWDRQGNEQIIAILNYFEERINKKNEDKEYMTTEIEHKFFQILEQEGFLNFNSKNIRYSTKIRLERELKQMLENEKNSRETKKKEAENKKKDEIDQKFEDRVQQEFKNVENNKEFNEKENIRKQNLDIIKNFYIENSQNRYKLYDRFYYTIKFQEEWKKCILEEILPIDKKKIDIHDQELSKNLTIFFEELLVKNKIGRDNEEEKQKYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T2F2</accession>
    <name>A0A8S1T2F2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0140339</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T2F2"/>
    <dbReference type="OrthoDB" id="285923at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8145627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="152" mass="17776" checksum="7BD07F7B6F9E8E38" modified="2022-10-12" version="1">MNLCDNSGWDIEEQCYLDTLFTGYLPKLERRISENSSHRIRTLSQPKQFNQQASINSLHHHRPTQSQAYQIGSFSANFYAPSTTKSRIRFKFTPTLTQEDQQKQKNISQSPIKKKPKYTIINMSQLLSVTPKVKMHQDSLNKVLERFSNKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T9Z2</accession>
    <name>A0A8S1T9Z2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0180085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T9Z2"/>
    <dbReference type="OrthoDB" id="297815at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="146"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8148477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="273" mass="32567" checksum="48B21A83DA14EE34" modified="2022-10-12" version="1">MKVFYQKSTQPQLWDVMNYEQFTQQTNQVFQLSKGTYSIKAKSNNQPIRTTKDLQLYGQQAGIIIEPHQQSLFSVLGVHLPETNNPQSHRIVKQKVPAIKKQLKTEHDGVESFKKILHIGNSNLSNDWRQIITRIKDLQAESYYFKNHMQENYGTFQEEIDALIDEANQLQLQQQQVFQQNTELLQKIEQTKKEIIEQQEQYDTIKNQFKQENIEYFIQSKQQQNRNQIKIFKYIDEFDSFLNENKFILAYGANLDIRKKLQYFKNKYLQLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TBF6</accession>
    <name>A0A8S1TBF6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0170285</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8148072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TBF6"/>
    <dbReference type="OrthoDB" id="2136082at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="45"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="115"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="292"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8148072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="559" mass="66759" checksum="51EB1D32ECC618AF" modified="2022-10-12" version="1">MSYYIRSKLTRPQTTSNHNKPLDNSSIRESRLIKQYTTPKMGREKENLYDENIKLKSQLNEQTQQLLQAKSKIQQLERELQRFENLIEGSIKPETSSQKVQIEKYGLIMKMKTKQKQLILELQSKNKELIELKRTMRFTNYQELEIELQNYIDETIRLRQKLEYNEQMIAIQSSAEPLEKQILHLEKIVKILQRDNQELNQKQLNNDIITSKLQIQLEESLKQNEKQLNEIISYEKIIKDLNEYNKQCNDLISQLQVQNKVQIQSQQNTTSDELFKIQSQLEQLQNKYSLEMDTKIQEIRSLKTEIQRLNLHIQEMEKLIENQEDKIREIEFSIYSPVSRVGQIEPPLQNGFQIMKIEMKSQIMKKKLPKVQFSDIREICMKVRLNLFKNRVQYQDIDKYFNESEELSIHQVKNILHKPPFEIHDHEQIKLLSRYLIEDNAEDYVMYDQDRTNQTAIINSVMKKIVGKYEILSSEEEQSILQSIQQKLNPSLIETIQMMLTKKKNLLPGTCEQCDLDQALKFHEIQFSHREQERFNLICFEVSNSLDIINYERLLAYFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TBY6</accession>
    <name>A0A8S1TBY6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0190059</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TBY6"/>
    <dbReference type="OrthoDB" id="310819at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37473">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37473:SF1">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="254"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="86"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="290"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="306"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="323"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="346"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="373"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8149338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="401" mass="48345" checksum="49B8B5E7856A63C9" modified="2022-10-12" version="1">MTDIHSQRKSLQKQAYIDDLFTNDNETDYDKDIIKLYNTAKRERKTIEAHKQLLDNRVALLKQEEIRTLKKIEETRKKALEIYYLKKKNEEKLKKKEEEKESLQKKQEQQQQIRKQQEKEHKLLIQKHMNEKQQKAFIIKEQSKKIELRKQNIQKQSLTSLKEKNQIFRESRDINRVMERIQLEKREAIKAQLGKKLQSEQQLKEIKQKEIDQIETYEMDLLQKLQNTQQMQKTAFEELESALTMTAKEFAEKYLQPKQKDDQNKEISNFNSDDEEANKEKERPHISEPSQDQSSKISIQQINEINDQHQQNDSKQDEDNDQNENQNQNSKEETNQQNQNNFQQDEQNKSQKGDQNEISNLLDSPKNKVEQDQQQQQQQQQQQQQQQQQQQQQQQQQQQDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TC94</accession>
    <name>A0A8S1TC94_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0180417</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8149104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TC94"/>
    <dbReference type="OrthoDB" id="302256at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8149104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="172" mass="20330" checksum="2533EBCB09A90B03" modified="2022-10-12" version="1">MIIMNNTPQRQKTQDSDLIDQLQIIEDSNFKENQVEYQNNLKIRNVELQQLRQKSIKTPTLFISTQTQFQSSQQISSKRGSLAELHSNLPSTQTYLGQMQNRKKREPPSLKLNEQQQIKRYESLVNLIQQPTNRNYIKSQNDYTTRNKDNINFNSQQVQIQQLIKTLGSRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TEJ9</accession>
    <name>A0A8S1TEJ9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0200195</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TEJ9"/>
    <dbReference type="OrthoDB" id="292008at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="505"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8150308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="561" mass="65938" checksum="992ED467CB1505B7" modified="2022-10-12" version="1">MIYYMIFSLLINLSMQSTRIRTKESYTTNYEIEHDTIKMAHKKQTVYVLNLTNAYDVDVFGDSVDPIQTPFLSLDNKDVELLEVKNQCVGFPKHPQLKEILQYKIKDATEDSYYTDMVTLSEYSNLFLITQELVLIQIKLRCSDSGWIAQESQSVFDLKKQLNLVNPKIKTNAQFSCPKKHLNCLIVTPYGGFWVSKFSDFDSKDIPIEPYPEQNILKRKVVHKVSTFGEYLVVAVGTDGVDLYTYEQEHQDIYKRSILHILTISKQQFNVTINIIGVKIQNDKLFCLDDTQGLFIFNISNVNKPILIMDIHIPRTVAFEVYENTVLVVAQTPNNIEYIMEIFIDLRDHSYFVNRVLVDDFTYRDLQINPNYAFLIGEEAHLIIKHSIFNGFVKYNKELVRTFFENQLVRFELYSKSSEPEEQRFKETFYYVGLSRQAIHVWKFMDYHAFLLCSFDERSEHEIIVKLNSTSCDENIKRETLYQQCQADQKINVLVSGPLFYSDTLTILVFILFFLGFVLCIICICVCVRWKRLLKELEENRYQLKEMKKYGYLPEQEQNQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TFI2</accession>
    <name>A0A8S1TFI2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0190269</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TFI2"/>
    <dbReference type="OrthoDB" id="299374at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="348"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="348"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8149752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="637" mass="74752" checksum="86AB20FF69D52AFC" modified="2022-10-12" version="1">MGNSDSNASIKALHTNYMVIRNIKDDPRYGEGRIVQQKQTKQEAFQKEINLTDEDKFKKFKVLLEKKESNKYENLINVTKLYFHEESQFCGQFFQIYILLEYIPQSLKETIEKRLTNKQYFKEKELMQILKGCIDALFILQSQNITHQAIRPETISYQEQQQLVKLSDPTVSGLLSSFQLVVQEELKQINQYYLSPQLMQSLNDEVQPQHNPYKSDVYSLGMTMLHLSTLNNCDDCYDIPKLKVLLQQVQRRLQQIETTFSSQYVSLLRKMLLLNEDQRPDFIQLKYELQASQSSQSQFYEEQQVIQIQKALQVQNYEQIIETSPIQLQSMQMFMNSEERNNLKIQQSNQSIKNSQIQKSQELRQSQQSNQSIYIERNEVKELRPDISDHIKILPLSEQIAEPSGIDDNIGNQIGIDKSIHITNEPEQQNILMPVTSFETSQQYEQFIKMQQSKSNYYITDQSQKVDQNMRFINSINTKRSDIEKVIEIYPNGSKYDGEKQNGLRQGYDNGGVYEGQWYDNKMHGNGTLFYASGKPAYEGQWVNDKFQGKGTLYNEEPQMLYSEFDYRDFDQVGEYWTKYVGQFYDDNKEGQGILYLSNGDKFDGTFIQDLISGLGRYIKVNGQIIQGRWWRNKLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1THF7</accession>
    <name>A0A8S1THF7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="1">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0240296</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1THF7"/>
    <dbReference type="OrthoDB" id="2420415at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031647">
        <property type="term" value="P:regulation of protein stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF644">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="3">
        <location>
            <begin position="96"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="390"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="596"/>
            <end position="623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8153581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="959" mass="113036" checksum="67AC86BAF493A0C4" modified="2022-10-12" version="1">MQQQSSQLNKPIDNQILQQEIRNGYNEYDIRNAWQATNGNMAQFKQFLQGIKNQSFIINPINQQFNDDIQVAMAASLTLHQQYIPFGKVRQNYIPCGLLNVGNTCYFNCLLQTYYFIYDFVSSIVKCKFDHINLQNIVDKRLINSIELVKNLQNLFVLMIGSDRQFVDPKKVILSICDDFGKALPIGDQKDVSEFNNYFLSRIDEGITYLSQSLQKTNNSQSQQQIKQQLPGQSLVQFPTINFNEQNQKSLISQKSLLIRQSSVIVENSIVYKLFYGKFAPQILIKGQTLSKDCQQELFNIIQIDVNSKSLIEGFDKYMNQAVEYKNDKDEYQQAIKQFWIQQAPQVLTFQIQRVEYNKEFGSFTKINSFFQFDKEIYLDRYLLSNQKMAKDIQIQNQQMRDRLKKIDQELSMLTQFNGNIDIIQNLETTLQFLRLQNQNEKQHPFYVDSNDNKTTIKSISEYYQHYIKKRDCLQKERKEIELKISESYNSLKKQKYILQSILMHEGNPDAGHYFAYIYNLDDQKWYYFNDIHVHEEIEQNVLKNAYGDQSNSKSAYLLQYVKEDNAKFAQNSMVKLKVFSTSEDQKRYLTDGYGLLLSQQQREFLNQNNEKLKQEVEQQKLFSQIEMIITKYNQYLEIANEKQRKYQQKQYPPFPVKPPYLENYAMYLKLKPDERLFKWTILDNAIIKIMPQSNGLFGQPGINEIVKTQILEEMNKLGNNKPQHFPSNQEHSNSIEEYKQALKLQCIIAYLFEQYNNNNFIEALQALHKYMKLAKLTNQQSFFYTLAINLKHLILLGIICKIMQNKNIGESELKILKLIIAFQMQEHFLYTEPKFWNDQLKNLIDTAINTYGVNEKQKQIQEVLKPMIQNKKDVEICSLLDASSQSIEEYIQNLQSIYLEQQFENNLEDFLLDNLMTTLQRFFLIKTIQLLFENIQKSSTILDKKVILDIVGGIPQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TNC4</accession>
    <name>A0A8S1TNC4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">cystathionine beta-synthase</fullName>
            <ecNumber evidence="4">4.2.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0240074</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 10">
            <text>L-homocysteine + L-serine = L,L-cystathionine + H2O</text>
            <dbReference type="Rhea" id="RHEA:10112"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:58161"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="4.2.1.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.22" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TNC4"/>
    <dbReference type="OrthoDB" id="10259545at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004122">
        <property type="term" value="F:cystathionine beta-synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006534">
        <property type="term" value="P:cysteine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009069">
        <property type="term" value="P:serine family amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000003">
        <property type="entry name" value="Cystathionine beta-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000118">
        <property type="entry name" value="Related to CYS4-cystathionine beta-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="7">
        <location>
            <begin position="43"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8153187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="491" mass="54290" checksum="7ED9A37CF643B9C0" modified="2022-10-12" version="1">MIQDLSKDTIQCTYKRGANEQPPTPHSKIEFVPFKKIYNDALDAVGHTPMIRLNKIPKEYGLKCEVLVKCEFLNVGGSLKDRIGVRMVLDAEKQGRLGPGKSLVEATSGNTGVGLALACAVRGYPLVITMPEKMSQEKQDVLTGLGAKVIRTPTEAAWYEPESLIQVAKKMATDDPNVILLDQYSNPSNPLAHYEGTAEEILWACDDKLDAVIISTGTGGTITGVGRKIHERVPGCKVIAVDPYGSDLALPQEVNKTDIKTYKVEGIGYDFIPKVLDRTEIDGWVKTVDYDSLLMARKLLSQEGLLCGGSSGATVWGALEWAKSQNFTENQRIVIILPDNIRNYITKHLSKTWMIENKFIPYDELKETGHPLAGRPITDLNLHDIQSLDVTTATIGQCLEILKTQPAVPLQENGKLLSVVFEKKILAGIVNKKLNHADLAKKVHSKEFVLVPNTLDLNQLERIVERHDVVFVEDGDKLKYVTPRDLLGLFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TQD7</accession>
    <name>A0A8S1TQD7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0250169</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8154064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TQD7"/>
    <dbReference type="OrthoDB" id="414826at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05380">
        <property type="entry name" value="CAP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035820487" description="SCP domain-containing protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="3">
        <location>
            <begin position="34"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8154064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="246" mass="28460" checksum="4651F1D9B34BF65F" modified="2022-10-12" version="1">MKFVLIVFLAFVYAKVGCDTDNKERVKERGMSKKKEDELLELHNEHRNDVAVGKQKNWQGKFQTASNMNYVKWDNQLARFAQDCADKCPANFRIDCSFPPQYGYVAYLGDVENGGADWTAKRVFKKWAQHEDHARQIETAQVQFFGCGRSQVSRKNGMSDEIVVCVYNRKPNLHGDVYKAGVAGQECLHGRKKGYLGLCKHSATDMSVLNFKHQKKNNEYKFHQQEVKHHKGHKHHKNNEGHKKLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TQF3</accession>
    <name>A0A8S1TQF3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2 NT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0250179</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8154084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TQF3"/>
    <dbReference type="OrthoDB" id="298833at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019448">
        <property type="entry name" value="NT-C2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10358">
        <property type="entry name" value="NT-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51840">
        <property type="entry name" value="C2_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 NT-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8154084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="275" mass="32115" checksum="120CF068D27441D9" modified="2022-10-12" version="1">MNYGNSLKTQITFIPIKLSLDLGVEAEVFLNFICGSHSLTTQNGCLFQQRECNFDYQQLKIVTTMNIKKGKFQEKKAKVRVILQYKNSYKKAGKINFDISEYMNQQMQQIQDEKLLIDCPDSSATLMFQLKFKQAVLNKSKLYPDIQQKLMEPESLIKSSIIRIPNIIEVQKFDKCNNCLKLQEQIKELKTQLQSNSQRSTLSQRDSIQISKLQMLENEIKCQYQIERELKTFIDGLEQELLKQKRIVAQIMQECLQMGDPKLIELVENVIQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TQM5</accession>
    <name>A0A8S1TQM5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0280296</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8156381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TQM5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8156381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="33" mass="3887" checksum="ABD19E8614C56F1C" modified="2022-10-12" version="1">MLTLQITSLLNEKYLGQFCSFTRYALIKKFMAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TR52</accession>
    <name>A0A8S1TR52_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2 5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0240205</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2 5"/>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TR52"/>
    <dbReference type="OrthoDB" id="1902587at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01926">
        <property type="entry name" value="cyclophilin_ABH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000052">
        <property type="entry name" value="Aryl-hydrocarbon-interacting protein-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000006">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000022">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase CYP95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0697">Rotamase</keyword>
    <keyword evidence="6" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="7">
        <location>
            <begin position="29"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="176"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="213"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="8">
        <location>
            <begin position="300"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8153419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="466" mass="52443" checksum="9CF458ACD9A2F0B6" modified="2022-10-12" version="1">MAEYISLNEDGGIQKLILEEGQGDQPQQGNVCEMFYTGKLEDGTVFDSNEGRDPFSFTLGEGEVIKGWDVGVASMKKGEKAQLKIKSDYGYGQQGSPPKIPGGATLIFDVQLVDFKEKKKQKWEMSDEEKTIEAKQFKELGTNAFKAKNYSEAIKQYLEAVSYFEAETEFAHEQKLASHLNLSLCYYYTKDYKESLDHATKVIQDKPNNIQLVKAYYRRAVAHSSLGDYTEAKSDLKAAYAIDPNNQAVIEEMHEVQNKINISKKKEKDIYGKLFQQSYYEEEATPVSLVENDPSNITTYFDIKIGDDEPQRIEFSLFKKSCPKTVENFRALCTGEKGIGKAGKPLHYKGCEFHRLIKDFMIQGGDFTQGNGTGGESIYGEKFADENFSHKHTGRGYLSMANAGPNTNGSQFFILFKDTPWLDGKHVVFGKVTKGIELLDVIEKIETEQDKPKVSIVVVDCGEIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TUS3</accession>
    <name>A0A8S1TUS3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0280304</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8156395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TUS3"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002895">
        <property type="entry name" value="Paramecium_SA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01508">
        <property type="entry name" value="Paramecium_SA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8156395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="342" mass="40078" checksum="9518B38D065B790F" modified="2022-10-12" version="1">MKSQLVVIKLLYENETSNKLKTNQELQIYNIDEYQLNNVINMIIQSANQSIFLNSRISNQTCAFIENFSQFRSKTCDDLLKLYYISYFMQLIESQCTTNGIDYIIFGQCSTHRTAYIGLNTQGVKICILDQRDKFNRGIKCSNFYSRTNLDCKQQLSECITNSNICILGDPCDEYTYLNYCIQSKNQLCLWIQNSCINYSKCEDAKLKTFYECQIVAPFCTSNIQNIYHLLFVLNMKMWKPVYLVGINYGNEMKMQILQNLLIIIFVDQIIMILYLMDINVFKQFKNLSNNLCMDQSIEGSRIQITKTCESIQNTFYMLKSKKLLQSQSNYLIFILKKPQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TY67</accession>
    <name>A0A8S1TY67_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0290310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8157044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TY67"/>
    <dbReference type="OrthoDB" id="301639at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="45"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="108"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8157044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="188" mass="22117" checksum="06CDA38F32FF05D0" modified="2022-10-12" version="1">MYQFRSSIYYEPSDSGSVKSVQVANAKQDQVKYLTDQLQAVIIFNEKLEKEITSLKQQNQQLKQSVLDKDKQIQDSLDSQSKTISKLHVLLSENKKLQEIVISTNKNLKNSNSIVQQLQQENNVLKSTMHQQEEYHVEEMKKRANELEEHYQLMQNIVSDLTQQVSQLCEEKQRLQYELEEKNKIQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TZE0</accession>
    <name>A0A8S1TZE0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0290329</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8157082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TZE0"/>
    <dbReference type="OrthoDB" id="10292656at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8157082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="90" mass="10763" checksum="C984062CD65230E2" modified="2022-10-12" version="1">MLNQKKGSTSSQTSQLHQLSNVFEIIAKYSILCSEDNYIQKQSIAQLSKYEKPRQRRSQSLLQQVLEKKSELLTNYQKRKYFMKKRKYNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U3A2</accession>
    <name>A0A8S1U3A2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0290346</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8157116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U3A2"/>
    <dbReference type="OrthoDB" id="310386at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8157116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="250" mass="29811" checksum="BA42F33BFA993E29" modified="2022-10-12" version="1">MIKIELTKSGDQIISDDQDQIKIEEINDESEDKNGYNIKEAERLFKEEVALDDLKKYQEAIECQDKAISINLKNDNAWFIKGYSFHFIQDSIECYDKAICINLKYDMAWNNKGYALHQLQKYPDAILCYDQALSININLQNYNEKLIKTQIYIQLIHYLNQGRNQKPNSYIWLHWKTDQMTQIIFRNNYQSYEALLYISYNNIKYVILINCPFLYCKDFLIFITYYKLTNQSLQNLLNLFTVQAEKNIFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U5F2</accession>
    <name>A0A8S1U5F2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Isopropylmalate dehydrogenase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0320198</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8158867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U5F2"/>
    <dbReference type="OrthoDB" id="10261637at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000040">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004434">
        <property type="entry name" value="Isocitrate_DH_NAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00175">
        <property type="entry name" value="mito_nad_idh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF42">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="4">
        <location>
            <begin position="34"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8158867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="355" mass="39719" checksum="0428E1696F74317B" modified="2022-10-12" version="1">MRNCIRSAFRQNLQLRGAWSQNYLQRPGEGALKNVTLIPGVGIGPEITNSVRTVFEALHVPIKFDVLDNFNFENDDSKKQLRKNECILLGVMTEKNQKYTDNYKFYKYLDLYANITFAFSFEGIIQRHHNTDIVVIRENTEGEYSGVEHEVYPGVVESIKVTTKQASLRIAEYAFEFAHLSGRKKVTAVHKANIMKLVDGLFLQACREVAQRYSFIKYEEMIIDNCCMQLVKNPTQFDVMVMPNLYGSIVQNVVAGITGGVGMAAGASIGKDHALFSQGCRHTGRDIAGKNVVNPSAMLVSSTLLLRHLGLPNFADQICRAVQHTIQDKNIKTKDIGGNATTDQFTSEVIKSLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U610</accession>
    <name>A0A8S1U610_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0330191</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U610"/>
    <dbReference type="OrthoDB" id="293269at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001578">
        <property type="term" value="P:microtubule bundle formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046785">
        <property type="term" value="P:microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032273">
        <property type="term" value="P:positive regulation of protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008907">
        <property type="entry name" value="TPP/p25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12932">
        <property type="entry name" value="P25 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12932:SF9">
        <property type="entry name" value="TUBULIN POLYMERIZATION-PROMOTING PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05517">
        <property type="entry name" value="p25-alpha"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8159523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="705" mass="82886" checksum="7EDDFF8979AA9DAF" modified="2022-10-12" version="1">MISKMIMDRQKKTQNIDENELDSFNRYLQRDNQPLNLGTQLSYSKIKVNVGMPVITEQQNETLGNTHQSTVQQSKNASPQKKVIGIEDIRLNIRIIFKFYASFGTRNNTRLLKSNKFIKMLADAQIMPNILSNRDCDILYAQQTKNNESLTLENFQNFIPKLAKIIYPQLNVQQAFQKLYNQNLSQLSQKILQFTEFGQQIQFLLQPISNQIKEFVEPIQQQLSNLHRFVFEDQSTNISKVQWQFMQFLTQCEILPNYINQSQAQLIFDNIQIRQPILNLQEYGNYIFGLNNFIESLIIISKTVPNLDEFTQFRLLLDKIEQSEGFSQYLRKLNRTQSDKVRLFYNIEKSRVYNKQENLIQQSQMIQNTQNNLSNTNIQIYNSNKKVDESRVNVSMMKQSKYLQDIDQSYISSLKKLFEFFAQNGEPSNIQSLKSTKFNKLLQHSGILNNLITITDSDMIYSKLCGVQSIKTQQKNKQLTNGKMTFGQFQISLGIIAEKCGMDINELIQQNILPLEKQMSDDNKEQILQILVDLLQDEQIIQLYEMIQPIFDWYFQEYTKQQNSILYLSEFLKFCQDFELSNILISQTQLTSIFYSVASLNQEGQEYTDQIFLDKSQFVEAISIIAIQIYSTIPNNIQRIVYLLERIFQSQNASKIYIKQNKLQARFQNVIEDIKYRFLEKDKIHQEDEQYNFDDLVNGTIQQFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U686</accession>
    <name>A0A8S1U686_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0330025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U686"/>
    <dbReference type="OrthoDB" id="296831at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8159237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="416" mass="48735" checksum="79F165E248AF7A66" modified="2022-10-12" version="1">MIFQFLLCFHFIFRNITIIILQVVKLEWELIVQMQLILKRQNELNNEVKVTKKSESHKVSSFSQQSSNSLIDGVITLKFIERMIQSRQSALLKNQRTQGFRYSQMIKKRKNKQNKLNDKAIVIQKNWKSSQIQRKLQGSQKIIIIVKKSKSKTIIIIIKQLSYSSQQVQKEIFNSLEFGMNNANNVQSREKRPLFVFKNGATYEGEWVGNKRDGKGVQIWPDGARYDGEWVDNKACGKGSFYHVDGDSYIGEWADDIVNGYGVYRQSNGAVYEGYWRNDQQHGKREEKWSDLSSYKGDHFEGKKLGKGKYMRQSGSYFEGDWFENKINGYGEYYWSDDRIYKGSWQNNKMQMVRTYNHKKFHQGDKFQQIVTQSMDIQLEKMCIVLGLVDFQSCLTTLFCAVLRQLSSRQICERGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U7K7</accession>
    <name>A0A8S1U7K7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ODAD1 central coiled coil region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0350163</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U7K7"/>
    <dbReference type="OrthoDB" id="303248at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051876">
        <property type="entry name" value="ODA-DC/CCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049258">
        <property type="entry name" value="ODAD1_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21694">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21694:SF18">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21773">
        <property type="entry name" value="ODAD1_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ODAD1 central coiled coil region" evidence="4">
        <location>
            <begin position="182"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="583"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="86"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="381"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF21773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8160770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="610" mass="72125" checksum="7B2798EB0374DB27" modified="2022-10-12" version="1">MNQTQKSFDVSKGGEKVSDIESLKRRTYKKILMMSSMNKNIMKYIQKAFQLGPKYLTIECLVEEVKTTPLNELERYGATILDKDRLKELQELLGKIKQMIDQEVKQLSSDQLVQLIKYFVLSPVEKTYIQKIEAELAETKNLDEQIKAAQTRQKEQQDQKIEEYRQEKNGQQIIKQNLQSQQHQISVHENKLEKANQKLNEIVAHNKQLREKINQLRKEKNMVEEISKNLEKELEDKKKNVEETIKSAGQAYYYRNKAEEELTKLQKKAEAQKKEFEQECESLNEKIQHDKKFKAFIQSKKKEQEYLDKLEKQIAENQEIIRQKSASNAQIDKEYMLSASKEQEIKDAFERIKQETGIHDKKKERESKQLLTVFIELYQNNQIMSQFVKELQETVEELERQIEEKKEEIQMYSTKGATNDNQRREQKMALSNKIQQEEKKKVILKAQYEKSIETINLIKKYLEEVFQAIDVDDETIKKLKSAAITEENMVHFLGILEQKGLDAIQEYARLIAEQLKLEKGEVHGLSSQVDDLNNIIAYENANIMNYYSQASQFRQECPDDVLSFNEEENDMKQRWFQEEEFKKQAMESISKRSGPKKPIKPKFKEREQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UCC4</accession>
    <name>A0A8S1UCC4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0380133</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UCC4"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000097">
        <property type="entry name" value="Probable serine/threonine-protein kinase nek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000957">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF13">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="8"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="316"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="468"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="468"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8162575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="520" mass="60592" checksum="7D493E07E56A272B" modified="2022-10-12" version="1">MESKLQNFEILNKLGSGAYSSVYKVQRKSDGNVYALKKVKLVDIGDREKQNALNEVRFIASIHHENVVSYKECFIEDNNLCIIMEYAEGGDLLQKIQRFVKKQQMIPEQEIWQVAIQVLQGLRALHHKKILHRDLKCANIFLYENDQVKLGDFNVSKLAKNGLVYTQTGTPYYASPEVWQDKPYDHKADIWSLGCVIYEACALKPPFRAKDMDGLYKSVLRGQYQPIPVIYSQELVQLIKTMMQVHPQNRPDCDKLLQFQYVQKKAKLYGIPLISEDIEDDLLKTIKWPITRKGLQTNKSELINLNFQLPGSNYLNQHNSNHIRRNQQSNRIKSQDTNDSISLNQPNEALHQLMKQITQLEQKDIEKSPNVNQNLNKSTSKQTIQDNILAQDRRLRVSNSTHEKHEKYESVQPKCISKDRIEHQIQINSNYYAKDVIQSLNKQHYSQKLPIISGTQRQDEDQYILRKNSSNERNPSNHIKRSKHDQLKKDLSMQPSAERPTVLMKIIEEHQQLPKIKKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UGD3</accession>
    <name>A0A8S1UGD3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0380261</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UGD3"/>
    <dbReference type="OrthoDB" id="10373054at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="48"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="406"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="639"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8162829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="742" mass="87740" checksum="9E605C87F1265BD7" modified="2022-10-12" version="1">MRGQNQPYYQICSPQRAPQRIQVTTQQPSQNDKFINLKCEKVLMGFEIQRQNQIIKELEENKNQLEQQIKTYKDEQKILLLEISNLNQEMNKAQEQEKKHREQLNSYIKNQPQLDNQMKIDLSGEYEKLKEENQYLESKISYLLSINQLENKQDISSIKKLNLDQSEAQNHIPANIQKLLEQLQNNIQNYKQGNSIQTKYLNSISILKELIKSQISYLTKQDNLNKEVDYQQLEIYLTTINQDIDELKAYETPKAVRRILNNQSPQLGRSCSQGLSSDEVFSKIIGHIEHLQQAASNIPTILKQISLLNSLVRLKNDDLKLISNNNKLKQSPTRFSIALTPVKYLSPIRISPLKIQPFIMNMSAPQKVSIIQSNIHDSHQTNQRQNVIDSFPIQQRDEFQNVLNSFRDNQDKINQLEFEKLQLQEQVKLLRQKYDQLIENQIQQPQDQNLRQLESLDAENLILKRQNIDLKQQVKELIDERIKLQQQYDISKNQYQEILNQLHQEKKPEDYKKQIVLQQEQIHNLENEKLMKNDEFITLMEENDTLKKNQIQLQDQIIELLKQLNQQNIQQFDEREVESIDEQQEDRLKQINILQTENIILKDQLAEVEQQRQDDLKSFKSIIGNGADQQCIADLVTQKQKLQSDQRQQLIQINKLQQELIEQQNENNQIKQKVNELKEQKQLIQRTQDYNWSKFTSPLNNWIVFIQWDYKELSKVFNQILCTTSPNIIQNNEGSIFENPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UHV4</accession>
    <name>A0A8S1UHV4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T0370278</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8162256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UHV4"/>
    <dbReference type="OrthoDB" id="287694at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000235">
        <property type="entry name" value="Serine/threonine-protein kinase ark1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 10 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 10 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="257"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="382"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="386"/>
            <end position="387"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="399"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="11">
        <location>
            <position position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8162256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="524" mass="60917" checksum="2827C931F09D11A5" modified="2022-10-12" version="1">MFQPNQSQIKYNNQFGATSAVYFSQQQTSRDKIIQTREGSENKQLLQYPASQIVYGPSMKFNSPVHSNIAFRKTSKELNITIEDPLVSQQVRQPSPNVKKQREQMEKDWNKLIDKSKTLQENLVLLKNNISMNLNKKQPDPRLTKESTITNTGLSSRMKLQKVNVTCKLNSPFKAQPKLEVCKSLSHNAFGNLKFGSNVNKNPTLINNTSSINYVNMNNNYAKKLLDELQSQKVNTQNKIIKQTNPLYIQIENKLKLKISSFLGRGKFSDVHMAIDNRTGLIFALKIIKKQTVIDHEMQQQLAREIAIQSKLQHPNIVKMFGQSYDQQYIYMMLEFCNNGELFQHQYKQPNKRFNEKEASNFIMQILSAIQYMHNQGFMHRDLKTENILLSLNYIKLCDLGCVREIPKYEDRRNTFCGTVDYIAPEVIKDEGYDERCDAWQIAILAYELVAGNTPFSEFPRDDDAIMENILNNKFDLPHFFSPSLKDFVIRGLQQSPDHRISIDQMLLHKWIVENIKTDREYLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UJ87</accession>
    <name>A0A8S1UJ87_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0410082</name>
        <name evidence="2" type="ORF">PPENT_87.1.T0410083</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UJ87"/>
    <dbReference type="OrthoDB" id="306463at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8164535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8164537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="126" mass="14847" checksum="03E76A642CD16C5F" modified="2022-10-12" version="1">MGSICKCQAFDLQSCQNELTFASKNKRQQEQMILKNKLGVPFQDENLKVFYIIYPKNENDFVQLFKQLEVINKNEQYEIEEYYQVSQRSQNNEIDSNFNLNGVSDGSFKQMHLTSDKSLQIMDQQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UMX5</accession>
    <name>A0A8S1UMX5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0420013</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UMX5"/>
    <dbReference type="OrthoDB" id="71437at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="62"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="106"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8165042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="341" mass="39229" checksum="669D52EAEC9EA01E" modified="2022-10-12" version="1">MQQEPSQKAIRYDLLPQCSIKQKSYCRAIAINNNNSLLLAGSQSIIKVYQFNQGMMKCIQYLNKHEKIVTTLNFFNQGYFISGSDDGSIIMWQKNLISKAKYIQSLKNHSKSILCVILNNKENLIVSGSYDTTIKFWNLPFKNLNQWSCTQTLSVHTQPVYGLSMNEEQTKLVSCGYDSIILVIEYQENLSTWMIKQNIKVDLWGYRVCFINNNMFTYQPQLSTCLYIFLLDQKSQTYLKSYNLAVKDGGSFCSSLFPSTFNQEKNIILDKNGCHLNLIRLTKNKNKLGNEEQILKLEQSINLGTDLYGIANIFGTMSKDGNYLISWDARTEQIQVRLFKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UR08</accession>
    <name>A0A8S1UR08_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-formylglutamate amidohydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0410277</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UR08"/>
    <dbReference type="OrthoDB" id="282777at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007709">
        <property type="entry name" value="N-FG_amidohydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05013">
        <property type="entry name" value="FGase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8164896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="250" mass="29109" checksum="1E8F8F35828BA80E" modified="2022-10-12" version="1">MQIQNYRQYENSDVFKYGNNKRLLFTCDHATNVLPQGLEWSKDDLTNFANTHWAVDIGALNLAKYLANQTESILVHSKYSRLYCDVNRQIQSESLFRQTGDNQQIELNQNLSLEEQWIRIQYHSNYHFQVRQTIHELKPNFIYSIHSFTPEYEGKKRDVEIGILTSIFDEFGEAHQQILKNKGYDCRINEPYTGKKGLNFSIDCACLTFAKPIVGTLFEVRNDILSNPERFAKVSADILATILEVIGKWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1USD0</accession>
    <name>A0A8S1USD0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HNH endonuclease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0460021</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8166702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1USD0"/>
    <dbReference type="OrthoDB" id="1883054at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33427:SF1">
        <property type="entry name" value="F6A14.21 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33427">
        <property type="entry name" value="HNH ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8166702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="142" mass="16451" checksum="978C29E98CDEA996" modified="2022-10-12" version="1">MSENNINSNHNNITHQHHHNQRRSPSPYNPRRFDKLAREECWNNAQIMEGRNPDRWRLDAVGNPICKALNGCRGPLCYQFDHIVPYSKGGESISKNCQLLQSFVNIYKSNKENISKDELKQVSPQLNFTSEVFDAIEYAIYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1USS1</accession>
    <name>A0A8S1USS1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0470002</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000047">
        <property type="protein sequence ID" value="CAD8167247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1USS1"/>
    <dbReference type="OrthoDB" id="312140at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="35"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8167247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="154" mass="16925" checksum="12BD11471E801818" modified="2022-10-12" version="1">MSVCFSPDGNTLASGSEDNSIRLWDVKTGQQKAQLDGHDEAVISVCFSPDGNTLASGSSDQSIRLRDVKTGEEIKSSDKNYKDILAQFKIPLQQHSHISEASNYITTLLISQEAIFQAKGALILRGEFINQSGIDLKTLFKQKGSCFLEDLKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UUW6</accession>
    <name>A0A8S1UUW6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0490114</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UUW6"/>
    <dbReference type="OrthoDB" id="290868at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8168451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="427" mass="50842" checksum="E3ADD55A7181FD3D" modified="2022-10-12" version="1">MKLKQFFIVAVKPIKINKTLERIGKLPSKYLLSPVLKEQNDINPQELKEQTNLDFQYQYQANKIGRQNDYFLTQEQYREFAYKNPHGKVHIFKYQDVNFTLAGSQWEHVPIKNLYKLLLFSRPSLIMLQMKPDQILKGFNYQFKSSKDYINQIIRDPEEIMPVGYHEKIAEILQHDKLEFQEYEPYINKERIDYDKIATISYYAKKFNAKIMLADIPALIFRESLCNQLTLPQLQQYFQIACMATPQNPDIFPNTPMNVAYQIIPHIFLKRSDEFMTTLIEYLAVKKQYKRVFGIFGNMQSDAIVDLLDNKCASHLKYELEIPKIKKTLFKDLTCEDLVERHAILDAMYYGDIEEEIDPEQQDYYFPETMAIIDKYADSQFIDKTRSAQMRYLHLEMLKKYSIFQQKYYKIGKSKLKEEYMEKANLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UW45</accession>
    <name>A0A8S1UW45_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPENT_87.1.T0490069</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="431720at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008331">
        <property type="term" value="F:high voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000309">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000087">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000093">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000117">
        <property type="entry name" value="Voltage-gated Ca2+ channel, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000162">
        <property type="entry name" value="Voltage-gated Ca2+ channel, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000166">
        <property type="entry name" value="Voltage-gated Ca2+ channel, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050599">
        <property type="entry name" value="VDCC_alpha-1_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628:SF7">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="3" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="355"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="422"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="460"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="927"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="961"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="988"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1014"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1053"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1124"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1275"/>
            <end position="1295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1315"/>
            <end position="1335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1356"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1396"/>
            <end position="1421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1462"/>
            <end position="1482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1502"/>
            <end position="1528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1587"/>
            <end position="1606"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1618"/>
            <end position="1640"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1647"/>
            <end position="1664"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1714"/>
            <end position="1739"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1805"/>
            <end position="1829"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="15">
        <location>
            <begin position="234"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="15">
        <location>
            <begin position="937"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="15">
        <location>
            <begin position="1279"/>
            <end position="1537"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="15">
        <location>
            <begin position="1584"/>
            <end position="1836"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="42"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8168403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2154" mass="254037" checksum="7D7D41B6ED13BB0F" modified="2022-10-12" version="1">MKFKFSQCKKHKLNKQAIRIKPKNDEESDQFFGLINEAISQKRPRSNSRQHKPKKLSKMEQKSQHNYITSLKEIQLTQNHNESYCEGIDEEININRSVLNFELIDAIEFENRIGRKQEQYKVYRSFEGMINEEQNNTIYHKYNQKSQENIRDNQTEYLKSQGQQEILNDYNVRSKRNVHQDNYNLILINSQGIEAILNNFQNYFDSYPLLKLRKYIVLLLEILSQICEIIILSRVYRYVMLILITINIILLITKYYSNFYEEYQQFFDTQQEVFFIIYVLEICIRVAGHNIVNFLCKFWNIFDLLNVVILGLVTYGLISFDFSPLRAIRVLIFLSQLSSQLQLTFKALIQSLRYMLEALLIVLIFCVLFANVGLHQFATAFYNKCVYLEEGLILTDQYCGWLDCPQYSTCINIPIIFAPDQITNFNNFINSFISIIRIITMDNWTDLMYPTLYTLSPLTWIYFVFVIFIVGFFAFNLIIAILKTYYSHIVANYSQDQGDVRKEQNDKIEINLRFIKDLGFYQKLKVLQRAKLHTQQQQQQLDCSQSQIISNYTIKQPFLDQQVGNQSIMSPQISARIVAKSTAKKIQRFTKNQIEKTQKKKQYAHVLSARQKREMEELNNQQKSNFIGKIKSSCKLRYIILPKRSLILEQTFQFQNSNEEEMEILHQLQTHDVLGWKVSVVFKDYISNSQSEVLGQKQEYLLKEKKLVTELQKIKSKKINQFYFDDEILKQYQNTCNKMIRNSILPINKTRRKIKLSTQTFEELRNDEIQIISPKRQSSKSSSVERKFRFPRKTMHTIKQQKSDFFFEFPEYQEGEPFIYLNGVKRNYESVSILINRTLENDQLIIDTDDLNTLFFQYLKYRRLEIKNNAICKQNISFLDVIKNKKLNLLSIFKSLNSKEIHIWMIGFTGLWKSIQLKIKMWLNNTFINLIFDFSNLLSFCVLCSIGYLDDQLIEQINFYMTIQLIIQISLKLISQGIMLFGYEGENILDSLIIITQIIYNIITIIDIPFKENYGSEIKALRATNAILLYRIIKYNQFVTRIMRIAKITFKKYLSLMFLMLSIILIFSILGMQFFIENFQENQSLSQLHSFKSFAKSWMTIFKISTNDDAVGMLKVVIKFQKPWIGILFYFITVFTINYLIYGLVMAVLLDAFSSELEMVVENQQDQSLILARKVGIYENIQFDCQKDNFTQNEEEIVQEQSTQPTKSHSSKHHAKINIRDLPETSKKLFMKKRKTQQFIYYEDTKCADSCFIFSYNNRFRKICYRISKSIPFKILNDVTIIISVVFQIISHFELSNNQKLFDSNLNGYFKINLFLQNIVILSIFIIEIIAKGFILNEGTILRDFWKLVNMTYTTAYFLSFISTAPIFKLAHQLIFCRPLMMVNMFNSVQGVKNAMVKSLIQIINIVAVILLVFMVFNVFAMHLYQQKMGYCEDLMNFHISYSECQKQNKKWINHPYNFDNFVNGLFTLTMISSLDAWGEIMQVCYNASNSNTGPSPFKIQWATYLLFVTFIFIGAMFFMGFLTGVLFTEFQKFTKKIENKNLTQDQSQFIQICSIVLNQKPGYSHAPHSQIRKLAYKLAFSKYYKYYYNCILVTNSIVLCFLFDINSQYTRNLANQLYQLHAIIFTFDCIIKIMAYGVTRYMASRWRLFELIITVVSIADFFYDVKKRWFHLFTNGSQDETIIYFARVFLICRDIKILLIFQEFKGLQRLMRVLFFSSSLLLKILYIQLTIQATYALVGTRLFGHITKGRAIDEYYLNFRNFINAALVLFKCTTGDDWRALIIDCSGSNMYCLDDIQQCGSGWAYLYFLSYYLLSNIIVMNLFVLALVDQFESFFSTTTNVIQTFVENIDHFCNCWCRYTYDTKGLKMHTRYIGRFLLDLNQPLGAQKGDNIWDAAKDAFHFRIRADKRSYVNFNELLYETLRFAFLEKVFKDGTPAGKAEMKLFDMAMKRKLNEKIKENQKGNEKQGDIKLFDSIDGSFDFKYSYQNFNILQEYLHLLIIFKTWKSYTINIFEKSKRGLQNYTEQSSDERYEYMQSYSVHTKDGLQVMNEEKLYASQAIDEQNKEEIMQNFKESNKYRLTNYNSQNSSTLHGVVYEPAGTNPTSIIRMQSTSLEAQNQSVQKSILKKKCQTLTLNDVRMYQNKQKSSENKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V3E1</accession>
    <name>A0A8S1V3E1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0560018</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000056">
        <property type="protein sequence ID" value="CAD8171968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V3E1"/>
    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051938">
        <property type="entry name" value="Apopto_cytoskel_mod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44145">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY A MEMBER 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44145:SF3">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY A MEMBER 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="16"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="114"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8171968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="160" mass="19577" checksum="3C999C138821005B" modified="2022-10-12" version="1">MQTYLRFFRFATQQSDPYLILGLNKNATMNQIKDAYIGLCKKYHPDLNNQEDAREKFSQIHEAYKTLKQKKFYEQVDEEFQKNYYQAQSEEECFKMIFGFYFYEKPQEYYKPENAQKRADYQELLRKFKSQQQNTSEMRNNDFHDIKINRTFNSDSIYST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-06-18" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V561</accession>
    <name>A0A8S1V561_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0550179</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="421226at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001266">
        <property type="entry name" value="Protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="251"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="354"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="400"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="1120"/>
            <end position="1377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="47"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="47"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="1149"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8171737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1680" mass="198233" checksum="311B5BA0EC5BDFD7" modified="2022-10-12" version="1">MNQIQQDTEEPISKLNQLMLTDEPIGEIQDFLNYSLSEGNFSIQGSRVNTNNNIPSQSSKHKAHESKRVTFDSSLSSPTNKFLMNKKNQKSLDPQQFPQQQIFSVIQFCFIKRFINRISWKKKINSQFNLYQYNIIQDLGSSFNLKLFRDSALNLKPMVSQFSQSSDGLQQFVKNKELSKKIKKQITYLKTCKNNLIKKLEQEIEKIPLITPESRLKIVWDCIVMMSRLYFLFTIPIDLAWDKYQIIYGELYIPTILMILLLVFDFILSFNSSFYQFGQIVSNRATIAKNVISKSYGLEAISILIVIIYAMISNSSQQEFNLLTEWNIPKLISQVEETLNLSKPSSSLLELFKLLLVLFFVLHCYSCLWYFVGYYSYLYSEKGSWLEFYHVENETWQVQYLYSFYFSTVTMFTIGYGDVVPISYLERVVAILYMMICSIQLSYSVSTVGAIIDTISAYGQEKMRKMRKINSYMQNRKIEYQLQYQIREYLNYYWESQNQVENDELNEIINQLSENLKEKLMNQSNSLILNECPLFQHNFSDALKSKLVNKIKQAVIQPENIINFDSIFPQLPPGQLFVGKGSSLGIISFISGKQSQERFRSVGFSKLLMLSRDDFLKVIQDFPEDYERFRNLYDSLQFEDLSVLQMKCFSCNSKYHRVLQCPLLHYIPDKELIIKRYCYSQSQNRNSKYERNPFRQRGYFAARFDQEVIEQEANSFNNNNWKWAEFYEEPEDDSLKKSDVHQQQQIGPILELSNHNLQQQSQNIINTIQPISDQSTKINLEPPQIKKKATLFKQRTKTIELLDIDDKQLKASLMSKARNQNIFNNNLMIIKEEDNQDNSPKDKIIQGSQSRASFSLDLYRKRPKESFQKMKKVVQMITNMNRMKKRQKLKKETKSIINQFLEVIQSQQLLKGLLSKVRIRIKQIQDGKKQILQEKDIQDTMLLEIKLQMQIEQLNEFQKKDQIQVMESYKKYKYYQVQNNLDQILEKMYFYKQQNTFDNQLRNYQSILLKYMIYPEIFFEKYRYQQVIIPKFELEESRRGSEQDSERTLKRSRMIKKSFRNLKLSQIRPMSDGTGKNLFTSINIYENDIVYFQSYYRKLNNYFMNNQQSRNFVGKKIDDYILVDVLGTGAFGQVFSAQNIITKESVAIKSVPKSKLREHNGLVGQLLKTEVQVLAQCSNINVVKMNKYLESENNCYLVMEYCNQGDLEQLWKKKNRIISEVEAIQYIKQILNGMRGLHEMNVIHRDLKLPNILISNNVLKIGDLGFAKRMESLDGVVKEALGTLGTMAPEIIEFKPYGILADMFSIGAIYYQMLFGVLPFSIKSYNDFLKDVKTNQPNFTRNNIKISKESQELLIRMLNPDPKQRLQWNQLYESPLFSNQVVNPALCQLTVEQVVAKQVDLKMNKQVYQQNQQKFDNINNQDMIKKLQSDVPKLEQKQVMDNSNDEKLLQEALQQKYLQQQKTTDLNNVFEKYLNERNTLVFLGTVLNEIHQRKHTQNTNVFLPAFIISKKLIIEMDRFLEILKTQRNIYQCAYFFELYTFPQFTAFVKLCEEDFQIQMTHFQVIQQQLQTILFQMQNQANEKYISELLPAPSAQFEENYRDILINYYMQLQDEKGESEDQDNAINNLSIYVLDTILFDRVVKKQLLTQKVDDYLEKFNFLTSKEKEEMIAQKFQQIYSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V6X7</accession>
    <name>A0A8S1V6X7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0590072</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000059">
        <property type="protein sequence ID" value="CAD8173358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V6X7"/>
    <dbReference type="OrthoDB" id="300157at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8173358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="305" mass="36319" checksum="A91CDDA189AFCC71" modified="2022-10-12" version="1">MTHIQNLFSLKSKDFFILQLIVLLKSKMQQDLPIESVLDTLDYGDKIKCISGLVATLEQMKSNQTHKTYLIKKIKELRLLSKIINDLLCKEELIHSPKRQQSKTKKSRIIFDIKVDLMLLKGSIIKLEQSIKESFKLHQEIHSKFLLIQHQDQDYKDDDQFEHLDIMRLVQIYQNEMEYVNEYFNRIENNPNNSNTHSLDILNKSYQEMLLDDNIIVITQYFQNLYQMIDDLENLKQKLIQYEEFESQSTNFENSRNSSTNEKHFTRTKTQTYELCEQQRINILKEITNEIKEKSSETCINCSIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VAD9</accession>
    <name>A0A8S1VAD9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0590174</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000059">
        <property type="protein sequence ID" value="CAD8173535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VAD9"/>
    <dbReference type="OrthoDB" id="289113at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024453">
        <property type="entry name" value="Peptidase_C92"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47112">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47112:SF1">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05708">
        <property type="entry name" value="Peptidase_C92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8173535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="427" mass="49916" checksum="2DE9145E9638EF56" modified="2022-10-12" version="1">MYKIQSAISSVQEDSFNNTNFKHASSIEIGNHKPQDQNLLRMSLMASVNVRQMQMQQINQQAVQTQEIIEEVQVDFEGMQNIDYRLAQSVITQNAESETIEADIAQQSKILQSGLLQCKQPDSYSGWIQKKSKSLFSSYKNKFCVFHDGILCIYENEERKRANVVLNFHLFNFKYFSNKQNNIVTEFGLKCEDNENVFQFKGQYANDWFYVIKNCIDTYRLQPKLQYYIRPFEDYYKKRLIHNQQFLEMAQTGDILLFQGKHISCKAQRMVTRSQYDHVAMILKYTNGSIYVLEATGTFGVGIFEWKHMIGKQWYELYNKVVYRQLEIQRTTDFLLKIEQFTKDNLGKAYSLTVKKLITDKSVMIASSEKNTYFCSQLIAQAYKKTGILQTELSATQFWPGSFSNEKQDLILTNAKLSDEYLIGFNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VAQ6</accession>
    <name>A0A8S1VAQ6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chitinase domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0600178</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000060">
        <property type="protein sequence ID" value="CAD8174004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VAQ6"/>
    <dbReference type="OrthoDB" id="10254444at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070492">
        <property type="term" value="F:oligosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46066:SF2">
        <property type="entry name" value="CHITINASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46066">
        <property type="entry name" value="CHITINASE DOMAIN-CONTAINING PROTEIN 1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="3">
        <location>
            <begin position="55"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00040976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8174004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="347" mass="41048" checksum="956590214AB7D2F2" modified="2022-10-12" version="1">MSVFNIALFGIIGAILLYYLYVSSQSISIPFELQQIQEKELEARQKESGNKNYQIPNFFFVTPWNKEGYRLTIKYAQKIDMVSPAWFSVRYNNVIDGKQVVNEQWMQEIVKANPSIAIIPRVQIELQESSFIQQINNPQLLETIINLVQQYQDKFHGIMIDTPYLSYIDAFDAQDIVKFLQKLKAKLNNKMLVLTLYGIYNPQQYKHSTLRKLFKIADYTLIQTYDYQIQDEDDQLLSPYQWVQDNLEHFMTYKEKLLFGIPFYGFKKTEHDKTHFIGNELLKLNASNFITEWDRSSSECRYKNELVSISYPCPDFLVYRLEIIKEFGRGYFVWEGGQGIELFYQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VAS1</accession>
    <name>A0A8S1VAS1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2HC/C3H-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0560043</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000056">
        <property type="protein sequence ID" value="CAD8172016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VAS1"/>
    <dbReference type="OrthoDB" id="449446at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026319">
        <property type="entry name" value="ZC2HC1A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049899">
        <property type="entry name" value="Znf_C2HC_C3H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13555">
        <property type="entry name" value="C2H2 ZINC FINGER CGI-62-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13555:SF36">
        <property type="entry name" value="ZINC FINGER C2HC DOMAIN-CONTAINING PROTEIN 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13913">
        <property type="entry name" value="zf-C2HC_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52027">
        <property type="entry name" value="ZF_C2HC_C3H"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2HC/C3H-type" evidence="7">
        <location>
            <begin position="404"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="C2HC/C3H-type" evidence="7">
        <location>
            <begin position="506"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="39"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="243"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="290"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="101"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="290"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="324"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="340"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS52027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8172016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="536" mass="63659" checksum="7B53916AA61C92AA" modified="2022-10-12" version="1">MYQGSNLSRTGQMAVQNEQVSNQLKLLKAKMSNRDTWQQGTNQQLDGYPHTQPTKPSQSPLYPKSDYDFYTMKQQQQQQMQSNSQPKINDPRTYNYQQLQQYKQQQQQILPPQNPNRNDPYLGMNALNRLNQYDQLQKYKEDPYKKTNNLNNYNVPQSYYEQQLNKRQQQQQLIQQPNKYYDDVLSKKQQSYGYQQNNNYDFTQQPILLQNKYDYMNNNNNYTNNGLYNNNLQWQNSNQMSWQNTNQNNIYNNNNNYNQQQQSSQTNQYNPYGNQINQNILQQQSSYNQYNYNQNPQSLSPLKQQQQQPTQQYKYSQHNSQPQFEKRERPFERQQDPISEQYKQTQQTQEQQYIKPQPRKQPIQQPLQSYNTNNKVINPIDELPAVAKKNQTNSIPPEEDDDADLLECPEGCGRRFKENALDKHIKVCKKVFQSKRKEFNSKAHRQINQEQAKLEKQGLVKDKIIEKKKQMAQNGDPKWKKQSEAFRQMINAAKQGGTVDIQPQDDLVECPGCGRKFSEQAADRHIPGCKKRNFKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VHU5</accession>
    <name>A0A8S1VHU5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0620137</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8175042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VHU5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8175042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="116" mass="14158" checksum="72B694DA1A79BBE9" modified="2022-10-12" version="1">MILNHQENLLFQKNTNQQIWVRTYLLHFKQIYFFNLERIQEYLKALFDLWKVITRSQAGKPPPKKPAKEPENELIRKQLEHKRNLHFYAQSQEKDIQGIKRLPLLSYLSCFRFRAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VLD3</accession>
    <name>A0A8S1VLD3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0690042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VLD3"/>
    <dbReference type="OrthoDB" id="317064at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8178204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="411" mass="48270" checksum="9013B1CFF72164B7" modified="2022-10-12" version="1">MYQPRRLSPASSSPLYRNVNVQQQNQIKLQLKQITPTNNIPPYVQIISQNHHKQNTYSSYENNSRKISKERESSQSNQSKDQIFNQYQNESLFQHHTQSLIQKSEINQVYSYIDNIYKTQKSEIFKVLDHQNKQIEFLQQIILNQTQQQQNTASQFLQLQTQLEENLSNFINKQIKDLSNRLQNTYKTELEQTNKHIEKLRAQIFQINITNSKLITEKLQTSSFNGTENLIINHSQSHKPLLSDNQTHNLDQNIKIIKNNYLIDKDYQTQSHYPLNDITNIQMKYIKSLQSSLQNSKLSKCSSSNNNKNQQEGSPIELFNEQNVLDSLDNIKVFDPQLQQQSDIPSDTTNNQLSMDLSQARMSTIQENENEEDECIYQIVENHQNQTKFKNLKKSKDQINTKQSKSKTLYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VSE0</accession>
    <name>A0A8S1VSE0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Calcium-dependent protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0720149</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000072">
        <property type="protein sequence ID" value="CAD8179651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VSE0"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000737">
        <property type="entry name" value="Protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001382">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="10"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="296"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8179651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="362" mass="43096" checksum="40D1E1E4417DB499" modified="2022-10-12" version="1">MIKIQNKYSYSEEDLIGEGAYSKVYKGLFDKTNEFVAVKVIDWSKVKDKYYISGFQREVRILRDLEHENIVKIIDMIHDPCLNREYLIMEYCDSQNLTNFIYQSGGLLNENVSKIVLQQLLTALNELIKKEYLHRDIKPENILIHKRTLKLADFGFSVKADYSGNQLFRENVGTPLYMAPQILENKHYSVKSDIWSIGVMAYQMIFGEYPWIADDPTQLLKSIKSQKLYFPSHISISDDFKEFIKNCLQYEEKDRYNWDDLIEHKIFKTKYVIQKNYTKIEHLQESINQIRQLQKKRGTPLEQLFTNLDMDGDKKLTFEEFQVLLLYVDNRMEINIQKGIFYRYNVSKNNLLNFNEFSRIFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VSF7</accession>
    <name>A0A8S1VSF7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein L19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0670223</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000067">
        <property type="protein sequence ID" value="CAD8177466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VSF7"/>
    <dbReference type="OrthoDB" id="304986at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.240:FF:000001">
        <property type="entry name" value="Ribosomal protein L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1650.10:FF:000001">
        <property type="entry name" value="Ribosomal protein L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039547">
        <property type="entry name" value="Ribosomal_eL19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023638">
        <property type="entry name" value="Ribosomal_eL19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000196">
        <property type="entry name" value="Ribosomal_eL19_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057259">
        <property type="entry name" value="Ribosomal_L19e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057260">
        <property type="entry name" value="Ribosomal_L19e_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006343">
        <property type="entry name" value="PRK08570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01280">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25476">
        <property type="entry name" value="Ribosomal_L19e_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01416">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00526">
        <property type="entry name" value="RIBOSOMAL_L19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL19" evidence="6">
        <location>
            <begin position="3"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="153"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="153"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8177466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="181" mass="21703" checksum="F5D51AF0B89A3CD0" modified="2022-10-12" version="1">MVNLRLQKRLASTQLKVGVNRVWLDPNEASEISLANSRMSIRKLIKDGLIMRRLRTIHSRARARRYLEAKRRGRHTGTGKRRGTREARMPTKVLWIRRQRVLRRLLRKYRAAKKIDRQQYHEFYLASKGNQYKNKKVLIEAIHETKQEKVRVDKIEKEQNDRREKNKAQRVKKTQNKLAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VTN2</accession>
    <name>A0A8S1VTN2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0720056</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000072">
        <property type="protein sequence ID" value="CAD8179465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VTN2"/>
    <dbReference type="OrthoDB" id="39591at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051651">
        <property type="entry name" value="DMTF1_DNA-bind_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46380">
        <property type="entry name" value="CYCLIN-D-BINDING MYB-LIKE TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46380:SF2">
        <property type="entry name" value="CYCLIN-D-BINDING MYB-LIKE TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="4">
        <location>
            <begin position="161"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="6">
        <location>
            <begin position="169"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="5">
        <location>
            <begin position="169"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8179465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="321" mass="38698" checksum="3A64AE8DE8AC70B1" modified="2022-10-12" version="1">MKKKQKQNEKFITQLKSQYDDIFQDQKTEQQSYSQIQKKEQQQNRTNILIIFANKQLDQQQQNQTLIQQPNSVAKRSWHKKMDNLINPSDMKDGKFSEQEKENIMQIVHNYQAQNNLTDQQLKDYIELKTLGHSKIWLEITKYIPSRTVDSVYKMIRRSLDSKNRQGYWNKEEEAELLKCIQQYGRKWREISKHINRTPDNIRNKYYQIGEHNHLFRNKSYWTIDEFLILVNHIHILSGYPLLLPNYDSTLKQKLGSDYENIKFRLNYRKMKANDKDIFDLLKTIVNIPSNIGVTIKWTEISSEIKTKTKDDLRQMWYKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VZE1</accession>
    <name>A0A8S1VZE1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0750238</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000075">
        <property type="protein sequence ID" value="CAD8181169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VZE1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8181169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="186" mass="22677" checksum="C6B7E311EFF7E6DB" modified="2022-10-12" version="1">MKMSYCPYSLQFLISFVNIYIYMRISFEFGFWSLYVPQQQFKNVPYFDQEFDDNQLKDGQFLFQDSQHNAQQRQQQKIRFQIYPTIYERKWVLNVLHLTSIIFIFFLRLQIKLNIVKMQQISQFIHKFSQEGEEKQQCIIARLNLNNYKGRLSKILIGNEEFYFWILTSITGRVNCLFCQRIINIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W2C0</accession>
    <name>A0A8S1W2C0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">COMM domain-containing protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0800181</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the COMM domain-containing protein 5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W2C0"/>
    <dbReference type="OrthoDB" id="310996at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017920">
        <property type="entry name" value="COMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037357">
        <property type="entry name" value="COMMD5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15666">
        <property type="entry name" value="COMM DOMAIN CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15666:SF1">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07258">
        <property type="entry name" value="COMM_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51269">
        <property type="entry name" value="COMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="COMM" evidence="3">
        <location>
            <begin position="142"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00016556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00093452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8183301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="212" mass="25559" checksum="A8DE08C84DAE54DD" modified="2022-10-12" version="1">MDYNMKFVELLKKHENHQMILQVALDVMLQEKNYKNQLTKYRDELRREDKILVTKCTKALKELAMRSQQDQTKALEKISKIDQQIMLYLKDQVLPIIHFDRKLNNYTFIKYYTDPENSINPQETIIETQHKENTNLESFEADFSKLEWKLEITLSSIKMRKVIRPLILLIFHTKKGVKKTMYLDYNQFQEFRKNVALVLRQIHIIESIQSNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W3D5</accession>
    <name>A0A8S1W3D5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0800124</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="312590at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="206"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="371"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="507"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1062"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1149"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1212"/>
            <end position="1581"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1614"/>
            <end position="2135"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2159"/>
            <end position="2186"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2212"/>
            <end position="2314"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2357"/>
            <end position="2440"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2485"/>
            <end position="2512"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2604"/>
            <end position="2673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8183187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2781" mass="332612" checksum="778687D7D37AC6A0" modified="2022-10-12" version="1">MNPNREATRNQSYRPKKQKESRSQAQTFENLQARIRDFSYNRLQNAVSNQPIRSDSKNCQLQRQDLQQIIKQILDNGTKSFNSGYKLKEYPYSPSLQSPNQLVQSPKQFEYSNEKQSNQKSVDPFKFETFGLHSDQHKKKDECQYSDDSYSNEIAKLKKQLEPISNVSRNQLTELVRIAQATINDKKRMQQNNQRFQWQMESEDSCQEFDSKILDLQQKLKQSNDQCGQLKLKNQNLNNQLKDTELQLLNFQEKQKQQDQDFCYLQKQIEEFNDINTKLRQENEKLEYEIKVQEQYINTQKQNNQLNELNYQKKIRNLEIDKSNLKDDFQKLQLQLSKEREESSSKDEKIFDLQKLIDSLKYQIQEHSIQSNQQLQSIHELNNQLNQISQEKQDQFDNFLEIKQTLLNQINQQEQDYQSQLAQLDVLKQQEQFKQENQQLKNQNKAIHEDLNKLKEAKEQLQLQNANLTTQVIQLLQQSEENQIIIKELESEIKNFQLYYKDQDIKSQQYLDKLEKQNKQIENLETSLNQQKNNIDQLNHQNLIYQLELNEANKIITKLQKDLKDNKDQNQVLHQEKQALIENINQLNIKNDSLDKQMILDNSQINSLKDKINQQRQESEQQKNELLKSFDNDKQLLNDQINELEIQIEKLNYDINNQKEQIENLNNQIKEKKISIQLLNENLENNQQTINELNDQLNYQENQNEQFNERIDTLITEISNQQKIITYLKQKEDALDLINDKYNQLQVQYQIINDQLLESQDNLQQQLQSIKEYQKQTLQFQDFIKELKQLLDVSNEQINQLQIQIQNKEEMLTQKNELIDSTKQELILQKKETLQLKQELEIANDQINELQNNQNVLENENSILKQDLNNIKLQINEQLNLNQLLNKNLEDLNNNCLILQSQNQQQLDQIKNCHNQIENLQKENQQLNEKIIYQKQEIIQANQRNEILQSQLNDSQQQQQNLSDNLHNLQMQNEELQQKYQNVTQENVNLKDKFAEYEQILSTIEHKQQDNKLIQDQINKLVNIQSINEKDLDQFEEKSNDEELDKKDNDIILIQTDQNQENVLLKIEIQKLNEIIKQQQDEIINFQKNKNNNQLIIGQKEQEQQIYQEQSNEYLLDLNDLLDQPINDIKTQSNNDRQIELQGGNFTNYNEQKNQNEELLKIIENLREKIKHQDLEINQLILENQDYYQKIEDLDVIQESQRKIEIDQDNCIKQLNQKIEKEQSRVIELENQLIQLKQQILDKTQIIDNQSQQIDQLKQDQQNLGFKLNDQITQNNIYCNEISKLNKLNEESQDQKIKLEQQYENNYQIINLLEKKNFEQEQEILQLKQEIVIMKEQLISLSLTKNTIEQNLQDLTITENILRNQYQELEQQLLTHQNENQIQLKTLQDQHFELQTQKDNLECQIQDQATQKNEKQQQIDQLNQNYSELNKVYLQQQQQISQLKQTINDQIQQFDNQTNKLFEMERNSLLEIKTLKIKIEEKEHELQQLNNQFQEEIDQLKNHQINENTQYIEQLQQINEDLQSKIKDTQLQYGLLEQKSLDLQKINNELNQQNKNQEQCIQLLQTTNKDQSQQIEEITNSQQLNLQVLVNTTQQLNQLSLKLQQLQPNILTKLQKLKVKILQQQELIKSLENANDQQNNNIFDLNQKFLKAQEQVNIINTNCFQYQEEISTLKNINKELEMDKNKNVESLEAQMFINKQIQQEIAVLQQENQEYQIKLKQLEIENQGSYFDTQKQLQDNLQIKSQNEFLNQELVKKDEQILNLEIEIQQQQQKYQINQEELINKQNEELKKIESSISQQQNNFMNNITNKLLEVTQKQNQYQEKYNDYFKLIKKQFKQDKEIQQDNQILQDNLKMERRIKEDLNSQMIQIQQQFDKLKNSIDEKDIEINSLKNVNEKLSQQYQNEKQMLQNDLIIIQDKNSQFIQQIQQQQDIINKNNEIMLIKDQEISELKQKLINYELEISQKIEEYGNQITQLSKLKDEAFSQLNNKENEIVQLSNNLEQQLLNQEREYLDKLEKLENIQINFLNKEEELKQIQLINEQSSNQIFQLQEEIKKQEQKLNLKISKLEANIEEKDIQISSKQNELNDQGESINKYIDRVTSDQQLISKQKLQIENLQKQIEDFNKEEEFTQLEFQQQLQISKLEQMPQNNDIQGQEIVQLKLVIEEKDKHIDQLKQNINELREFKLNKSIISNRSKRSVNAIQIDQLNDIEETSQNSEDEDQQILELIQQRQRQINETDQDELNQERAILSKLREENDILRRKLTDQEQELIINNQRNLDHEQTISNLQNEIKGLKDKLDSYENILEKQMNKSILSNRSKRSQNCLQLDKFIDIDQISQHSEDDDLELQQFIEQKQRQQKDAHIESQEAQKLREENEILKRNLQDLQEEYKINNLKVTDHLQTIQNLQIELQEQKEKIENYENQYKEIKMNKSILSNRSRRSQNQCQFDQFLDLGDDEQNLSDNDSMLEQKILSQQHIFQLKEQELNVKLNQLQEEIIQLKDQNKSLQDFINRGSTPSPIQNTESDPNKYSKIILEREKLIQDLQNQLNEKDQTFPNNKSKNQFLFDENQDEENQVGDDLAEDIKLEQQAKNDFENDQQEHIKYLDNQNYDLLNKINNMEEENKTCKNLIDKFEQEKQKMEQQIYQLKSTLKELENQNKELSIQLQNQIKIVELSQKLQSEQMRKLEEQPQIFEQKNDLSQDLITLKNQYEQLKGLCQQFFSAIFNKTPEIAESILGIILSMIEMADKQNICQELFFKSGEKKDKKNKNKGIKGWLDKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W4I3</accession>
    <name>A0A8S1W4I3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">DNA topoisomerase</fullName>
            <ecNumber evidence="3 7">5.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0800097</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the type IA topoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="3 7"/>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W4I3"/>
    <dbReference type="OrthoDB" id="430051at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031422">
        <property type="term" value="C:RecQ family helicase-topoisomerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03362">
        <property type="entry name" value="TOPRIM_TopoIA_TopoIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.290.10:FF:000001">
        <property type="entry name" value="DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.140:FF:000005">
        <property type="entry name" value="DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000380">
        <property type="entry name" value="Topo_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003601">
        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034144">
        <property type="entry name" value="TOPRIM_TopoIII"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390:SF21">
        <property type="entry name" value="DNA TOPOISOMERASE 3-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390">
        <property type="entry name" value="PROKARYOTIC DNA TOPOISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52039">
        <property type="entry name" value="TOPO_IA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Toprim" evidence="9">
        <location>
            <begin position="30"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IA-type catalytic" evidence="10">
        <location>
            <begin position="192"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="661"/>
            <end position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8183135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="930" mass="108362" checksum="A22A11BB2941A500" modified="2022-10-12" version="1">MAMIFLYESLKDFLQLMNKFKTALKLGKIKVLNVAEKPSVAKSIATVLARDYKIEETKSQYNKLFKFNFKFGEQEAEMWMTSVTGHLKNLKYPKQYQKWDQWDPLIILKDAEIENIIPYEKRFLEENLQRFAKECSQLILWLDCDREGENIAFEVLEVCKDSNPQIQVHRAHFSAVTYVDIKKALENLKQPDENLSNSVLARQEIDLRIGASFTRFQTLLLQQQFNLSSIISYGPCQIPTLGFVVQRQKEIDSFVKEKFWFISCEDQTGCQYNWSRGNIFEEMIVLLLFERCFTEQAKVTDVQQKEVQKWKPFPLSTIEFEKLASKKLRISAHKAMELAEKLYNRGYISYPRTETNKFPPTINLQNIIRDQTNHPVWGQYAQNLINSNFDQPRAGTKDDKAHPPIHPVKMMSESEAQTTQEWDIYQLITRHFLACCSKNAKGSETTINLQVKDESFYKQGLIITEKNYLEIYPYDEWSQQQVPKYNKGDVIQINLKVQNGKTSPPKPLTEAELIGMMDKNGIGTDATIHEHINTIQEREYAIKKGQIIKPTKLGLALVESYEVLGFTLHKPHLRALMEQRMNEVAQGMKQKKDIVEATIDEMSGILKELQLKKNLILSTFGQYLEALKDYDENNDDDNTQNRELPQQNQQLYQPQRRNNPTQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQSSNFQSQCNKCGTQLVEIKQINGSILEVCLKCRQGQEKSQNNNSSNYQSKSFKTTNIEETFKCNQCNNQMKIRQSKLKNTQFFGCLGYPQCNQAIFLPDYIKSIQRTEKKCKKCHSILFQTEYSENSTIQQKSQLLCLYPGCSEGLKTEWKIQNKESQQTNINFSKQKQGYLSKQVQCKKCNGFGHQEDVCNYNTKTMVYLTDTKQEQKKNKLCPSCGIIGRHPKGSNCPMKRVKKKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WCP3</accession>
    <name>A0A8S1WCP3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0870007</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000087">
        <property type="protein sequence ID" value="CAD8186322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WCP3"/>
    <dbReference type="OrthoDB" id="9973183at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBC core" evidence="1">
        <location>
            <begin position="10"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8186322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="157" mass="18422" checksum="6414A8997981907F" modified="2022-10-12" version="1">MASNIFVLRHGQKRILKEINTFKLWQLSDIKIGLIDQKDIYNLWASIEGPQETPYQDGLFNLSFQYPNEYPYQAPKIMFKTPIYHPNINQLGEISLDILMDDWNAALSIQTTLLSIQSILDSPLLDNPIEPEIAWIYQNDRSLYNKTVRMWVKKYAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WGE8</accession>
    <name>A0A8S1WGE8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0900008</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8187717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WGE8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8187717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="67" mass="7549" checksum="9C0186981B2734B4" modified="2022-10-12" version="1">MQSFTLKGSVGSCYSKLITFNQFWQNLKERGLILIGNGGKQFFQGLLNQMIVFNYLIACQSLIALRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WGM5</accession>
    <name>A0A8S1WGM5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0910164</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000091">
        <property type="protein sequence ID" value="CAD8188483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WGM5"/>
    <dbReference type="OrthoDB" id="10372194at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8188483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="163" mass="18989" checksum="9150BE2353641F59" modified="2022-10-12" version="1">MNKKEEELIALLTGLAALEFIEEQRQKKEQQELVLLLSLLALQQHQKEKKILQLLSCCQDDCKCKNKKKKVKTWQHHCGGNVYQTNQGTIMCEKCGSEKPSHFWYLYCQNETADDYNYPINSFDNCIFNCGESLKQVKDRQVAIDFLKEYYNALKNKIGNGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WGU1</accession>
    <name>A0A8S1WGU1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CTLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0910023</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000091">
        <property type="protein sequence ID" value="CAD8188211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WGU1"/>
    <dbReference type="OrthoDB" id="1926212at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050498">
        <property type="entry name" value="Ycf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE SPINDLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="277"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="311"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="381"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="715"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="824"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="domain" description="CTLH" evidence="4">
        <location>
            <begin position="895"/>
            <end position="938"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8188211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1089" mass="128055" checksum="B284F4F7395DEDEE" modified="2022-10-12" version="1">MLQKYQSILSLTLLIQNNQYLYFHNFQIKFQQNVIYNLSNRLARPLILRESRHTKINQKEPPILVLKLGSQPHVGKLPPKVLQQNPTTEVRRIKNKSISQHISGNGTHQQVNQYRQPNKRIIYDSTNYLNPQINRSSSNRIKKIHSVSSHINDSFNQHLDDNTRNNIVQNEITTNSELDEGKKLFIENKVDEAQKKFLNYLNKYGLNPEALYLSGLCYISLDQEEKYIEQFSTLIMTFPRFKRTSYMYLAHSLKKNNLINEAIHVISQGISHFNRYFEALTFRAKLFIKIKNYEKAIKDFNLAIQINPRKSVCYVGLSECYKQTNQIQQAIQELNKALEFEEQSKQKSILLKRFIIYLDNKEFDKAEQDMNLLFEICQNDSEVYYFKGILMQKQKSLQDALLAFEQSIKINSDKKPVTKALYEIVKIKIDQNDYYSAQHELDRVIYLDVDNKQLQKFELFVGGVIYLMKRNFEDGVQLLTEMITKNHQSDNLKFQAHQYRAYGYFCLSKFNLANQDLELYQENLLEKASLYNKFLCHGIIKYEDNELEQSKKQFQLAHKILPNKIEPLFYQSIIQIKKYCSSLEKSSEENRIKYLKNALNLLEVASKICEQSNLLFYKGILHFAFGNIEKSALDIEQAIEKSEDNHAQHYYVRGLLHCQRQLFKQAINDFSICLSLDQNHSQSYLNRCKLLIMMGDVSTAYLDLQKYVELREQYSINYYTVGILYFLIGAYDEAIQAFNNCKTLEGNYEKVKVFIYGKELNSALKELTMIAEEMHNQEALIDQQVLTILKETSLQISTNILEEGINKIIQIQKMQQEGLIFRSSDIYFYKGLFYTYLGQYSKAKQCLRFSYDLKEKEIKKSKNAQLINQNNLEDFDFINKTYNIYEHLYNSAILDVMLGNIDEALETLTLLHEHLTQIEFRVQLQILMELLRDDLLDKPPEDKFSQIQEFQLFPQHNRLCSIYPAIQISLKKYSLLIRMSFCLPIVEFPSQPILFDEQLLQKISPKVVENKPEAPWIKRSNEGVIFTDNIQCIDELDLQSTVRKEETEETQQEISDLDDENEINKLKEKLKLDDLIAKKLNSILQQNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WHC6</accession>
    <name>A0A8S1WHC6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0920083</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000092">
        <property type="protein sequence ID" value="CAD8188773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WHC6"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010182">
        <property type="term" value="P:sugar mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044793">
        <property type="entry name" value="SIS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47179">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SIS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47179:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SIS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="583"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="655"/>
            <end position="699"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8188773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="714" mass="85447" checksum="19E8DB689021B7F2" modified="2022-10-12" version="1">MYIYIIFIEIVCGSHLYSRSNGSVENQIFRECSYSGLNSDYYLFQCIQNYIFVPKLELTQKQIGIHSFERNGEIKLSNFLICDLITNEGQKTLEVRRILFPQHSNLIKKENNLMFYDLTQIDSICVIVSLLKRLDNQDYLIEIYPMFPGDVNQFVSISYKFNSDSNRSIFGFSQFYNKIRNALLLIILKQNQIQLIEYSLFDEKFREVSTYSLSENNVPLNWKVWQSGYIIITYEQFQVLYSIGEILYEIQEIYRFDSQPLQMLTNFNGTNSNYILQQNMKRTKLFIIEEIEQQYQSKNVRFIQQFDLQYSNIAFLIDETQLLLIYDDFETVQTNIMNLRLDQIQFCQYDIEELPEMNQQSINYQQQFDPDIYSQSKIIKLQQDCQMPCDLIYESLNISLVPYKSECIFESLYNQFLHGCNRFNNCYTCMQQMGCEWIEDKCQTEQNYERLSFNQVKESSKWFVNKILKCGQEIEFNQTYYGSVSKGTVFTLFHDATIYQEFNLVFNLQVEQSQNMNFIEQSFCLGNDQLQLCNQIILNHFNSDFRFNGYYFRIIFVILDDIKINELTITIIIDTEYYRIVKLTSWLVLAVILFGTIIYIANKRMNYLISLSLQNQQQKLDTESLQNVMEYMIKEKVIVKEHFSQQILQYDQEKCPFCIEQYEIQQEIIQIFCGHIFHLECFEDWVRINTKLVRCPICNQTIEYFLKHKEQFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WIS3</accession>
    <name>A0A8S1WIS3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0920023</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000092">
        <property type="protein sequence ID" value="CAD8188655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WIS3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8188655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="214" mass="24666" checksum="F129ADA9EB1D656E" modified="2022-10-12" version="1">MQQLDCLTDNCYWDADSSICTNKPCSEFSQSDCQILPDPFNCIWNYTTNKCENFTKCSDYSFNIDKLSLYKNCFWYQTPDGTQCVQNTEQQICESKLINKCSDYITNESCNSFACYWSDFNIFTEITCSILQQDNYFMYLSVDSKSVKLCQWTGNACVDLDVSTLTQTQCLTATDFTYGWNMDTQRSEVFEPPDISSSSYIILLEAIILVILIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WLV6</accession>
    <name>A0A8S1WLV6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0940103</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GOSR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WLV6"/>
    <dbReference type="OrthoDB" id="295583at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005797">
        <property type="term" value="C:Golgi medial cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048219">
        <property type="term" value="P:inter-Golgi cisterna vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023601">
        <property type="entry name" value="Golgi_SNAP_su1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21094:SF2">
        <property type="entry name" value="GOLGI SNAP RECEPTOR COMPLEX MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21094">
        <property type="entry name" value="GOS-28 SNARE- RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="239"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8189591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="257" mass="30593" checksum="3290A1D867517253" modified="2022-10-12" version="1">MNKEQDDQERQPILIEKDNQFFDWETFKKQYYQQENVLQTTLKQFNEFHSDISRKLIQLDKSDPICEEDEQDNRKQFQSIDIKKYQDQFDDLNLIMSTMSRMLNEYTISPEEVQAKLKNNMLRRFKEIHQEHEKEVLKLKQAMKTNIQKYELFKEAIINQNTTGNQKYKRDYQLNDFLSRGVNQASHVLDMASTIHSNLQDQGYSVNSIQNKVTDYIGQFEGINQLINGIRYHKNKGQLILMGLIALLVLIILFKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WQM5</accession>
    <name>A0A8S1WQM5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0980049</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000098">
        <property type="protein sequence ID" value="CAD8191037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WQM5"/>
    <dbReference type="OrthoDB" id="301963at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="122"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="189"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8191037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="615" mass="73164" checksum="458C5A56B6178EBB" modified="2022-10-12" version="1">MNRTIRLNQRVPYSLNQSMTNGLPVLNQDNTNFCTSQFYRRRISQKYQDSPSNEILTGRVQLPFQKDVEQLKEEIMSLKKENQKLKHLNQQLCFQIKLDEKEQFEKRTTQELSDDQITSNQNQRLAEKLKQARLLIQEKQIEIDNLKKSTRYMRLQEMENELCTTKLKFEFLNKQFNEILKTESDVNKFQKLTVQTIELEEQLRQFQNSHQKQKKKILNLRQELLTCQAMKDKYKKQYEQAQTDFEKFKKDHEQEIQKKNKYKETIENLNQQLSDKQGKIQSLENDLNKQKMLVSQKQRQFKELEDQYQTKKSWLTNKREAPSIFQEILDEKVNKNNIIITDDDEMISSVKVGSDSKIVFDDDIKEKSQIIPNRISIESAKEEQKPQQIINSIQETEQIKQKLPRVNFCDVENIGKFLKYQLLQEKIPIEDIHSFFNQKQEITIIELVDILSAYPFNIQDNKQALLLARYLIEDNSQQFVDYRPQQLNDVCIIKSVLKNVIGKYIIMSEEQQQNIMLSIAQQVWKFRQLIVEQIKMNLQKKTNSQFCEFCDENDFKSALQSSNIVLDDKQKEFLGQFIFKEFNGNKLFDYKGLIDKFGFTLQSNSPSKVDIQQPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WSY1</accession>
    <name>A0A8S1WSY1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1010084</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000101">
        <property type="protein sequence ID" value="CAD8192312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WSY1"/>
    <dbReference type="OrthoDB" id="10051290at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001106">
        <property type="entry name" value="Aromatic_Lyase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10362">
        <property type="entry name" value="HISTIDINE AMMONIA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00221">
        <property type="entry name" value="Lyase_aromatic"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8192312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="471" mass="54504" checksum="335FA4B2E9FD58B9" modified="2022-10-12" version="1">MIIQLPQKYSINLVRDILSNNATIEFDEIQVQQSRESFLTFIKNSQQQIYGFNTLLGQKMPQPIKDLQNYETKQLKQLLECNSSDYLQQDLCKITLFVLINNLIINSGISLNTLRQLKDVFNSHIPLNLPSTNFNTQYYDGSINWAILQIIKNISEYDTIIITFTKSLSFATLLYNTHKSLKYIKMAFITTSVSFEATNGVICAFTQLVNEVKQHQGQMEVAQELYNLLTVDDEESDLNISLKRITHNILQDAYSTRCIPQVIGAALDQIKFIINQIESQEIGQRAYDFEVDSNTKSIRDIIYNEQNNNETELLLIDYLAIAVSEIGAITERRIHRLMNPKLTKLQEYYEEYLRDEESLFGYQQLANQASNLIQKNKKYCHPVSGDSMPQTTHLEDINNNFEDSILKMQHITYNTIQILAIDMFLSSRIIKKLEQKEGKLIHSKLDTVVQQVLPIENTIQVAQWLEQFIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WUE7</accession>
    <name>A0A8S1WUE7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1050026</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000105">
        <property type="protein sequence ID" value="CAD8193754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WUE7"/>
    <dbReference type="OrthoDB" id="302315at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="507"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="465"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="515"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8193754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="628" mass="74100" checksum="DDD47B0A302B503B" modified="2022-10-12" version="1">MFLWPEGYNRQPQNPLKQLIEKTQGDILLLLEAELFVKQARLGDTLAVETLVQGTVGLLEVMFMDYKHVLSVHFEQLTNQYTIMWELIDLKLEKVNAQILTHWELVFQLLNNEQTVEIQWYFALRIIQLVSQEKSTLLDNHQDKLIKLYNHLEYTYVAQLIIELMSTHMNQYQQQFISKGIQEFEQLTELQAINFTQVIHELFTKAKNKDLIDYIKTSQILTKMFEVIQQTNKIVTKNAAHIISIISNYYSGELQNLDLEEEESEQTTTQFHSTMFYQIIKTNLHIVNNIIKDHCDNNKKISHLIIKLIEIVDNLVRITDVSLWECMHQTEIMISIIRLCNTYPKSDILSSQIERIIFYIFERALNDYHPFWAFQLIKVYQIQNNTNSMFNRQVFSFEQELNTKITKELDYITYDTQILEINSELQQSKIWGRTKWELKLQQFEYQKKLGSQMQINEEEAKLIENNDIKNNVQDIKEEVENKLEQNKEEQNQNLNGMNEDLNQQNLVKDSESDSSDSSSSSNEENQIKNDKSYMTVSANLEQIAFQDRKNKLNLEQIDKIRKKSIQFEKISLQMSQAGRRLSLQAGLNIKELSPTYSEFKLDLEKKVLRNINDNSILKVDEMDSIINQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WUG6</accession>
    <name>A0A8S1WUG6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ADP-ribosylation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1030031</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000103">
        <property type="protein sequence ID" value="CAD8192952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WUG6"/>
    <dbReference type="OrthoDB" id="284243at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00878">
        <property type="entry name" value="Arf_Arl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001915">
        <property type="entry name" value="ADP-ribosylation factor 6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="29"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="128"/>
            <end position="131"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8192952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="181" mass="20654" checksum="B40F0AF15CD6B024" modified="2022-10-12" version="1">MGCLTSFSKEEDMSIALQSSKKLKIVIIGLEGSGKTSILQYLKNGKFTETQPTIGLNVESIQFKARHYLIFDVGGKVRTLWSHYYENLDGLVFVVDTTDSERIEIVKNEFKKLGSEIKYKIVLLIYLNKIDLPRSSPVELCKDIQQQESDIVMQRCSAKTGEGIWEGIDKMNKLLDHKFQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WVU8</accession>
    <name>A0A8S1WVU8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1010061</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000101">
        <property type="protein sequence ID" value="CAD8192279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WVU8"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000810">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8192279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="275" mass="32401" checksum="A8AD0107985CAA53" modified="2022-10-12" version="1">MKISNQIIGTGLTCKVQVVQIQDKNYAIKIYKDNYSLKLINKEINILKQLNHTNIIKIIDSNIEKKYIITQLLNDMDLFDIIAKDQKPFLLKSIKYFSQRLSCVIQYLHNIGVVHRDIKLENILIDEYLDLKLCDFGFAEKMDQNFVQKCQGTLGYLAPELQTIGLINVQDLPKADVYSLGVCIFILAFAHPPFKADLKSCPFWNLIQQRQWQSYWALVDKQNKFNQDFYSFMEGMLEQDPQNRFTIEEVVHHPFLIGEWKEEFKTEIRVRLRQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WWK6</accession>
    <name>A0A8S1WWK6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1060094</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="409374at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035911481" description="EGF-like domain-containing protein" evidence="5">
        <location>
            <begin position="20"/>
            <end position="2015"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1654"/>
            <end position="1676"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="167"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="389"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="653"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="698"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="738"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="793"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="1021"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="1075"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="1142"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="1285"/>
            <end position="1320"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="1331"/>
            <end position="1359"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="1452"/>
            <end position="1494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8194238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2015" mass="229570" checksum="2561157E0807A3AB" modified="2022-10-12" version="1">MIFWLLLLNAQCELLVIHSSLYAKTPSCGSAGCNLDGWTQQASPNYYTCVNEGLAMNYLDSINKQMYRGLSYGYPKVQFVVWFDLFFMGTWNNEAIQVKYNNVLFATITYTNPTTYPYSTEFCDSKQYQVISKKVLIVGQSNGNLLITNMSANGKVVIRNLIFAVEKCHWSCRTCTNQNSCQTCFTGTTLNAFGLCTQCAEYLDPSLGCVPICDINKYPNSDKFCVDITEQSILNDQFKNPPPNVQWIRQYDPSGLTTISKCSQYFGLLTGNEGMEKKISQPTQYKHKLRIKVVLYLYNTIPLNQGIFFTINNNYIAQLYNSNSVITMHNGYILRKTSQSCAGVGSGQLYTLIIISPIPTGDFIMRIQGNMQITTTVWGVTQIQIVAGTCQQECLTCTSDFICSQCIAGYYIYQGLCTNSMQSDRLSLNTTHVITYDTEYPYSTYLVSDFINYNIRQDPETYFTLISQNFQNFLTGFQTFGLYTKKIFGGPYVWTNAKFNNIYSINNAHYAISIRMKIILGDLFAQQFIIEFDTNGQEVITAGPLSINLFGRSSTEYSIDKYWYISHSTNSLTITIECQGNGDLLHEYCGISDYYIVVHQCFPFCLVCTDSTEIGCTSWDANYYTAKFSQVDCLSNEYYYNYNCNPCNILCSSCRNYNLCDTCINNLVLINDLCTCSQGYYFDTGDQQCHQCDPLCQHCLQYNFCIVCLQINRRYSNNGVCECEESFYEQLGNDVCIKCDNSCLTCNGPLQTDCTNCNTIKNYDLKSDGQCLCKSHYYLSYGDCIECHLSCKECFGPNQNNCLECDSTKTLYQLQCKCNLGYFDSGISISCNDCPIIENPSFPECYFDCNGTIQWFNINCTYAPVCLSGQSIVNYKCQSICGDNIIMSDEQCEDLNNIMNDGCYNCQYQCPLSCPNCNSGSCTDICGDGYVTGTEQCDDGITTIGCNGCTYQCQSQCSDCQMGRCFECNVSGYMLDIINNKCIEHCGDGVIIGSEECEDSNSNPNDGCDNCKFKCRSDCLECTTNGKVCNTCLNSGYKTVTGSYKCSTICGDGLRISNTFETEQCDDGNLIGNDGCSSTCQWQCQPSPICTTCSNNLCSVCGSGYTLISTKNKCVPICGDGIVKTSEICDDGNNIYFDGCHQCQLMCQQECVTCTIMGCTLCEIGYTLNLFNNKCISLCGDGYVNGNEECDDLIHQNCNKCRYICDYGCISCIYGICYRCQLNYNLNQQTKQCEIIIINQSQQLYQQQQLNNFNTYQLIKYCKFQIQSDCLECESNFKWNPYNQQCQINNFNCQQNCLYCKQEQCIVCQNDFILINSICIEDKSFINTISICKQSCKYCLNDICLQCNKGYYPVLDTCQPICGDQIKTLDEQCDDNNNIYDDGCFDCQFYCFGNCLNCQFGQCLECEEPEKLIISKMQCYDPEKCDKGYYVSDYDNLCYSKCGDYIKTEDEECDSKILCDNCILICSSNCKYCVDRICYTCEDEYTLNEHNQCQFLQQSQQLIPNLFNFELNNTLCFDDYCYYQSQPIMKLQLINQTNNNIQFLNLFFDQEIKFNCKNIQDLIKIQLIDDDNTFINSTIITQSQNLIIQILYLQTVYNPIIQIKLNDRKCIMNQFNQTLQDAIIQLRTESIILLTESEQLATDILQNIANTIQYIIIITLPALLIVGQISNFLNFVDLLQFTQYQQYINITQTPNMQEFLQVFKNTSFNKILSSFSFYNPYYIPTQFQRNGSFITTSIPITQVYAFTILSHYALKSISKLMRQSTLDLRTQNKLLLKFHIFGNKFIHNLNKNNLSMKLQNLTVFCGLEICMNAFICSSMGISNIEQIVEFSISNISSISYFLYILNTVKMPTLITLRSKDNKLNPIYKSPEKYRINAHIYIQSLKKLLYPLCLVYCHKNPTVTSVGLFIIDFGYGLYVYEIKPFKNKLENGKIMVQQINSSLVNCCNIFQSINLLTNPKILGFIQMGLIAESLTHSFFNEVAQKKDIIIKLFKKLFHYSDGKVENPGIIEFLVYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WXQ5</accession>
    <name>A0A8S1WXQ5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0970035</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000097">
        <property type="protein sequence ID" value="CAD8190626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WXQ5"/>
    <dbReference type="OrthoDB" id="302829at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8190626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="132" mass="14805" checksum="D1E519FE1D5FDE2C" modified="2022-10-12" version="1">MGNQCCSQTSNITQSSKEILNPIRQDSGIKSLGDSIQIGQFGDLIQQHCQLYQYQDGDGLLSPPPLSPKQQHVIVYLEPKIVIDESIHFQNDVMKGILSARSHECYSDSACSSNNCRDKPSKRVQFKEQSIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WYU2</accession>
    <name>A0A8S1WYU2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NOT2/NOT3/NOT5 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1040041</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CNOT2/3/5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WYU2"/>
    <dbReference type="OrthoDB" id="293823at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040168">
        <property type="entry name" value="Not2/3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007282">
        <property type="entry name" value="NOT2/3/5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23326">
        <property type="entry name" value="CCR4 NOT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04153">
        <property type="entry name" value="NOT2_3_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NOT2/NOT3/NOT5 C-terminal" evidence="5">
        <location>
            <begin position="162"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="13"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8193349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="291" mass="35235" checksum="61D6F94E0ADECDFE" modified="2022-10-12" version="1">MIQQSGISFQSRQTQLKDQINMLEQRAQEINQDIKKKRKTQGGQDRQIKLKTLQNQIDKMKQLNEDLMHLVIDDKKWQKLMMLLDIMYQNLDNPEHEQDINQLINQLYQTKMVPTVWNDKILQPNRLTIEPFDQKAATQQAQLVDQNSAAYKIKMMDFSYNDRPQKNDYYQPPTFKEKLNLNSLLPKYKMLPNSVFSQLSSDTLFYVFYYHKEPTEQLMAARELIKNQWLYNTKHGLWMKKDKHYQYKDEENEKVIKGPFFYFENEGKWQQKKKQDFSFKKKHLIQYEIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WZA6</accession>
    <name>A0A8S1WZA6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">PA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPENT_87.1.T1050079</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endomembrane system</location>
            <topology evidence="11">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000105">
        <property type="protein sequence ID" value="CAD8193860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WZA6"/>
    <dbReference type="OrthoDB" id="10045365at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02123">
        <property type="entry name" value="PA_C_RZF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000050">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056858">
        <property type="entry name" value="VSR_TRX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044744">
        <property type="entry name" value="ZNRF4/RNF13/RNF167_PA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22702">
        <property type="entry name" value="PROTEASE-ASSOCIATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22702:SF1">
        <property type="entry name" value="PROTEASE-ASSOCIATED DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25011">
        <property type="entry name" value="VSR_TRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="2 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035737209" description="PA domain-containing protein" evidence="13">
        <location>
            <begin position="17"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="396"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="14">
        <location>
            <begin position="59"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar sorting receptor thioredoxin-like" evidence="15">
        <location>
            <begin position="197"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF25011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8193860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="457" mass="52166" checksum="451E902D0D7DF1F1" modified="2022-10-12" version="1">MQFILLIVCIVAFVDCKMKVLRPPDLVDHLGSKVNIALANFGQIPFGHRLMGYVDMAQPNDGCSPLQSATGSQFIMIERGNCTFVTKVRNAQNAGYSLAIIGNNNDEPLQSDFVMADDGQGYSVNIPSIFISLRDFNIMKEYASNYKVLDNSDDKIFILVKFDVEKKDQIDVIFSLDVNDRDSYRVVDEYSQYYNLLQNENINYTLVYQIFNSNNTELDYIIDEDNCICSRRYCSIDPDGEGIASGRNVVEEAIRQTCIFKLFPDKYFQYMNQFNFQCTKAQAYSTCGSKIITNLQLSANEITKCRDESFKDPLNDQTTKNLSNAYNAILEHQLIVREQAGMVGLPSVVVNSIVYKGQLTGKGVFGEICNSFIIPPLVCKSEVENFYQFEHQSQHYLFLVILLVSILIAIFIIVLFFLFKKFVQRDSVEVTQVQVNEMVSQYIKFYEGKDQQKQNSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X0E3</accession>
    <name>A0A8S1X0E3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1060080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X0E3"/>
    <dbReference type="OrthoDB" id="313045at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8194210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="572" mass="68055" checksum="C9CAEDA1551FE03A" modified="2022-10-12" version="1">MTNFIEESMIINHLTSPQNQKNPNQQYSNLKPYYKRESTPNSNHSSVLNLLDIKKPQVVEDKNSENIVNPMSSQSNLSSDNQRQIDSDLELKQVLTEINDPDCSYQCNLKESQIYKESEIKQSKHEVSISEFQQQQFEKHKQIILKNNKHLKFAHSFNVYANQNQLQPSSPVNHIQLNEIRQEYINLDKDQLKNFKEIYNFYCKQPITTNKYQTFEKLQQLSNTMILQKFMIFCKDFKLIDLEISNNLISLLGGKINNLRKQQINYNYKNNNRSNFILTKFYLVQMYKQNADKNMELNFDNFIILIQKLADLLFPQQNYLLYELLNLKNPKVYRKKMTLIGKPFNTKEQSEIITQEKLYERKNIIPHQIKLKSESMRKQEDNSPIQFPKITRRVNTYKCYSPCSNEKHKIEEGNSLQMEDLIDYKQDIDQKRVLYDYQYIDKEDDYNLQEYQYSNQRSNRKKMIVDQFNISKINKAQQFSLYSNILGSNHKCDRLSSNSRNQIEISQNMIASGITRPSNVKSFENQENSSKKSPSYSPQTRELCNLEKKYLIHQAFANQQYYRDNQVKKTKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X0S2</accession>
    <name>A0A8S1X0S2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1050159</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000105">
        <property type="protein sequence ID" value="CAD8193999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X0S2"/>
    <dbReference type="OrthoDB" id="303271at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8193999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="287" mass="33651" checksum="649A7AA2E48BB36C" modified="2022-10-12" version="1">MINQKISDAKITKFPYSSHKTQSDTNIILCHSIDQSPKTQSNKDIDTDKLSYEKMFSSNEVDLEFEDFDTKPTNSYQVVSPGLSITSYDLDQTQKSSDIQLYGSVSYNQREIPQIVLAFRKRRPAQVQISQASYFNQESWKTLSNFSSPDSSPFQNKKIQIKSFALIPLKDEEEDEIFSVFNDPYNFYGTISQTKLESMNHLQQFLTLYKMRTGTFPRQRENLENEIIISIAMNLAKIDKQLFKQYFPSKRVYFDDENDYTRTKVIKRKEQRQLTQVVTPNFQYIQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X3R5</accession>
    <name>A0A8S1X3R5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1080169</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000108">
        <property type="protein sequence ID" value="CAD8195189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X3R5"/>
    <dbReference type="OrthoDB" id="287904at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026749">
        <property type="entry name" value="Tmem135"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12459:SF15">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12459">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 135-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8195189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="202" mass="23221" checksum="8F26B456C090ADC0" modified="2022-10-12" version="1">MSSYVISAADQLIIQSGTPFSCEIVHPGQSCEMNILSNLPRIMKSNSKVYLPVHLIPFLLYKRKQLMKNPISTISRALVSYFKSICFLSFMVQMLRYNWCKQKNLLKKVDPFVPLSGGFMSSFALLLESNTRAMEICLSIVPRFCETVINLLKSRGKMIDIPHGDVIVFSFVIGIIHYYYQHDPKSLKSTYYKVFEKIWGIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X5E1</accession>
    <name>A0A8S1X5E1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPENT_87.1.T1110100</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000111">
        <property type="protein sequence ID" value="CAD8196060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="298291at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008331">
        <property type="term" value="F:high voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000500">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000504">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050599">
        <property type="entry name" value="VDCC_alpha-1_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628:SF7">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="3" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="166"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="221"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="250"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="370"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="471"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="499"/>
            <end position="517"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="529"/>
            <end position="546"/>
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            <begin position="590"/>
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            <begin position="668"/>
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        <source>
            <dbReference type="Pfam" id="PF00520"/>
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        <source>
            <dbReference type="EMBL" id="CAD8196060.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
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    </evidence>
    <sequence length="1757" mass="206971" checksum="D570312FD66D5A0C" modified="2022-10-12" version="1">MLQSFLNCFKKQPKFRMNRDYGKDILSSVQDILPFSFQRHQYNKQNINQIGSHNKEFQATNDLNRLNQEQGIFSPNPSQANIRLHFLENVDEQFQKNENPYYCQFSLDYFGMNNKFRQYCILINESRVYQYLVYLFTSFCIVILMIKPYNNQESGFGFSLNNDQIIGVIITADVIFSIHHLISIVSYGLWSCKYGYYRNPIAFINGIALFFALILRQSYFYTLRILFLLEFFMHYKILRIVRKFCLTVEHSFISIVYITIIICFFFYVFAVVGLQIWTGILGNYCINKNNEVDFYPTRFCGLGRSCSDGYECLQGEFEQPFIQYSHNFTRFDYIQESMSSLFIVHYIEGWVEIEQSLADSFNKYVSTGYLWTYIFICSFFLQNLVVAIFLKQSQQEMHLDEPVQTVLFLNSHTKISLTNEKRKTVFQSPNYLSKSKQRVMMMLMNSTKRNNDQRKIRIIIKSILQSKITQYFYSLLFLANLVVLSLNDESAYKNNLEGTLAKVNIIFLVIYLLEDIARIYIWKQKESKIVVLLEFICDFIGLIVQVNQMEHPFILNTIKGIRLLWVFNYQTSWSNYRVLLSALWKSFNNIPIMILILFIYGLVIGLMGRNFFAGKLRFNDLGFYDLNGSYIPRANFDSMRETITTLFIIISSDQWQTIFYTTLETGSWIPYVFFICVQFICKFFILPSFLTLMLDNYEDAKNEADLSKARATKVISTMQNKSRIVPTKETSLLQVSNSQSDKKRFFGRLSKVSDDSDKEESHQKQHQILRRQQSKYYQVQILKSGDDSQNFSVSSLNRKEFFANSSLFILHNKSNLRKKLHVLIQQKYYDWGMSFIILLNLILLGYDRPLRQEYLIIDIFNYAFTGIFILDALIKILAQGFYWNKLEPQKSYIMNNNNQLDFVILIISSLDDLNIYKSQYLKAFRALRTLRILITLRRGSQNEQLNLISRSLLQTISLLSSMIFVTGISLWILSIICMTIWKGKLYFCSQVSLKDFKEIITAEDCLAKGGQWINNITNYDTIQDSLLCLFRLIIGEGWTQQMFSVSDITDRGLNPKINSSSQYQFFFYVLIFSLNIVLLNLITGLIINNYRTIKENIANFQQLNEFQRQWIQMMQIMQKKKLIYLIPKPSNKYRLICYHLASNQYFETVILIFLIVNLIFCSSYSGKQNITTQEALYGMSIFFIVIFHIELFIKLLAYWVHFFRDILNKFDLIILIMTDVLLIMKDNFDIPRLYTIPLIIRSLRIIKANKLFRFNKQLRVLIDVIQDLMPTLLSVIVFIAITIIIYSLIGVQTFSTIWSTSSDPQYPYTMEENQRTKSFSNFLDGVLILIDISTGQYWSLYMADYTIDTKGCHTQTPEQFELEGARGCSTIFGYFFFISFLVLIRIIMTSLFLAMIIESYQECQLENTAAINPYQLEDFLVKWSDYDPQGTGWITPEDFAFLMFEINPPLGFKDENSQYYDFAAAKNQKLKSHFYNHKKKMRLFKTEIFQKLSDYQIQIYQNQMVHFKDICIQLAYNAIKKKNELKGIEQIEDKIILRQIRKSWESRYPDLIGKKTQQFVVDIFAINSITDILRGVIERRKIKKRIKEMQKEKNLKQLEINENAQLTTKSILQRYKGRRRSQVRQRVYNFVQTNSFRRSEINVVDSESGGIHQQKRTVYQNYQQQQQNKEDKSSLTNLNFFIDPDEMCNGFPMKVVHETQKIDFPAIKSDKSLGTLQLSQSEKQVKKLGGYISEKCSDEENQQDSVIQNFKRNDMFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X891</accession>
    <name>A0A8S1X891_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1120024</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000112">
        <property type="protein sequence ID" value="CAD8196236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X891"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <evidence type="ECO:0000313" key="1">
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            <dbReference type="EMBL" id="CAD8196236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="104" mass="12478" checksum="FC4B11BEC29383F9" modified="2022-10-12" version="1">MSQEQLIIIPTNLYCFQQDLKFSLNEMQSAYQIYQFKQQHILLQDLKFGISFKELENRDFYLSANSMLIKTIQVSNSKYIEFMEMIQQFDVHSKLVNFLFLGFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X8V5</accession>
    <name>A0A8S1X8V5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T1150095</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
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            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ABC_MTABC3_MDL1_MDL2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ABC transporter B family member 20"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000194">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF16">
        <property type="entry name" value="ABC TRANSPORTER B FAMILY MEMBER 4-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="45"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="91"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="309"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="722"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="780"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="848"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="879"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="964"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="49"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="375"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="725"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="1061"/>
            <end position="1312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1193"/>
            <end position="1215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8197416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1315" mass="146391" checksum="ABA98AAD3FD63A7F" modified="2022-10-12" version="1">MSQSKGPSEVKIHASDKLSEIKTNQEKPKMIAYFDLFRFAKKEDIALMVLGSIAAFINGGAIPSFSLIFGSMINSFQEAGDEMVRQAGWNALWFLIVAIGTGILSFTMFSTWMISGERQGIEFRKNYFKAILRQEVGWFDTINPNELNSKVANESFAVQGAIGEKVPTFIMTFSMTFFGFLYGYIWGWQLAIVITATLPALTIVMSIFSVVIQQSAAATQAAYSEAGALAEQAINSIKTVKMLDGEDYEHQKYYHLLEGAAHKTMKYDLGVGLAIGLLWAASLWSYALGFWYGAKLIADQTYNHNQGDVYTVGDVMTIFFAIVTGGFSLGQAGPCVQNFAKGQAAAAQMYEILDRQPKIYNPKNPIKLLDFNGEIVLKDLKFNYPNRPDQVVLNGLSLRIPPGMKVALVGESGCGKSTVMQLIERFYDCDSGEVLFGGQQGINVKDLDLIDLRSRIGLVGQEPVLFATSIKENLLYGKIDATEEEMIDALKKANAWDFVSKMEQGLNTYVGIGGSQLSGGQKQRIAIARAILKKPQILLLDEATSALDRTNERLIQQTLDEVSKGITTIVIAHRLSTIQNADLIYVIDKGQVVEVGTHQELMNKHGKYEVLAKNQINNTEEETQKMKTQNSKQKLQPLNQVDVEKNNTQKMISMNVMENKSITEEVVDQYKQLQELDVLIKSQKETQKSTDIKYDKVNSNDKPQEPDAQMGRLFTYNQSERLQFIIGILAALANGCTFPLFSLFLSDIITVLAQSNPKQYNGSIREEKMSYVKSEADKNALYFFIIGCAAFLLWAIQSFCLSYVGERLTLKLRSDTFRKLLRMPIPYYDESKNNAGTLTSRLAVDCKLINGLTSSIIGINLANFASLVCGLTIAFTSSWALTLVTLGVTPFSFISGVLQAKYLQGFSAQTDEAYKDSGNLIMEAVTNIRTVFSFGNEQIILGIYSKKVQMPLEMAKEKGYKAGLAMGLSQMNIFIMNAIVFYVGAIFCRDIDLSVNDMFRTIFALTFATMGAGNNAAFAGDIGAAKNASKNIFEILDSEDEFQREERLKKQKLTKSIEGDICFNNLTFKYQSRDKNVFENLSLTVKPGQKVAFVGPSGCGKSTLMQMLMRFYEPDQGIITINGIDITDYDIRYLRRQFGIVSQEPVLFNGTIKDNIKYNISQATMEQIENAAKKANAYDFILNNQFEETQAEQKGNEQQRGQCFDRQVGPKGTQISGGQKQRIAIARAVLRDCNLLLLDEATSALDAESEQLVQNSLNQLMQGKTTIAIAHRISTIKDSDVIYVFEEGKIVEQGSYQQLVNQKGSFYKLEQGISQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X910</accession>
    <name>A0A8S1X910_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1150125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000115">
        <property type="protein sequence ID" value="CAD8197476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="299369at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="244"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="301"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1105"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1302"/>
            <end position="1324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1417"/>
            <end position="1436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1595"/>
            <end position="1614"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1626"/>
            <end position="1647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8197476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1690" mass="199363" checksum="D5950514C2A37200" modified="2022-10-12" version="1">MKQFNQLKNFNYYVASYHILLEMNFINLNKLWLYLIHLISEFQIFGIIVLNLNEELLQLPLMNIDYGLNIISIVSRPYKLIVKDNFFLYSYTIPLAVLSFYIIHQFFTYYQFSNQSQKSIMEYVMKSNNIKLIFHGLNLIFIAIFDKLFCIPCIQLACYSVQKNISNESFGALEFINTLFSMIALILIFWIQLLYLMLVKEAITLQLFNFKVLLFQGLDYFYTFLIYIFVILDCINLENKVRNYLIFVLFIISSILKLNQLLTKYQYVSDIQDFLINLNVAFIIISIILIGNQFIQDKQNLIIIVVIIPSLIITIVQQIKKKIDFNMLVILPENQSTYTQQYLITVFSQKNQLNCIQQFSLSLFVFNHKNNCQDKSCQCKKTGLMENDIKLRQLYLKQLILDFGKSINRLADQSLRGFFSLFYIQSLIAINQSVKAYQQTNILLLKQNVHQINDQQNIFSEQTSYSITVEENQNVYKKKSNSDMIHRLEKISLNQISYINLIKLSIILEIAKQNLISSFSFGNVVQKTQLSQSVQLFMKVEEQHQQLKQNIINIINRKKDIFMIMSSTTRLNPERFLHQCLDLIRKINFMENELSHLFQEFPSKKMQSIYSFYCAEILSNFLQAYRIINYNAISDNALIKIQKNYQVDLLTTQLHYMILVMDHQGTGLQIVERSHQMHKIVEYEQQEFKEIKSILSLLPRGFSTIHKHLIEDFLISGRSKFFREQNVNLILQKDSFLSPVDFFFDFDLTKLQELTFQVFFSETNGTNSYLILNNKNLILGVTRELCKQLKLQEFEFQKLPELFYLTEIQQIIPEFYQLIDQNKIDKSQPFSNVQIIFNDKNQNGQKSLQTYIDQMKKNPECIRFYYANIVVTIRLTHSIIEIKNVVESKKSIQQESIDQDNFEQECYEFIEELHIQQPNLFNNDLFPKLKKEPEIQIECFIQEEIPEHIDQIDTNRVVSIQQRQLLSESIQQYDLISPGKSNRQLIDKTNSFVTHQINFSSQQRFFQNQIEEKEKEQSQSNQSGAEKGFAKLNYEQNQQNAEIRENLRQQLNEENQTSIHAKKLNLDNAASSTLGTFSQNFQLFKKYELLQQIVSSVKFSIMFDLMIFSLLIVSLISIIFAIVLINNSSTDIYSALSQLQMLEFYTSFMNPCYLFLSSFASTYNYRNGTFDHNQQQNIQSLIQYNQYVINQSFVNIKQSYANQSKGSLLQQDLSTTLIEFKHIKDNSFTSEQISLRESIFSIIQYQYNFQTIFIQDNGIEKMMQQLIQYIINLNDINNEILNLNTEIITYISDSNLLLQHKWIVLCIRCIYLILLFQLSAWLLYIKHIRKYSKLLKLFQKVDVVWVLRDLERCKELLSLLNKDSNLMFRYKFNIFLKERFFKSELSKKHIIQDKIKRTGFIKLDTKQKLLLSRLGSFLFYSSLFGIFFIFSFVMNLQGIKFMDYYQIKSLQYNSIGELGLSIPKAYSLREVLYFKSNNFVGYEYISQNLTEQYLQIIQSSLDSLSTYLQTYTLLESNYNDEKLLELNNKTLCEDESIVKTFDLQQQYLCKQIYDNVMDRGLSITITKIRDILLTEMNNSQSFTSRINPPFNEIEIGIYLSVIIMNVLQTIKAGLSEEADQLNMTIQIVSIIYLVFTFLKIGFILLFVRSHYLNEFQNVKKLTILLPQAALFIDDLFERQLRQLIAKENLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XBT7</accession>
    <name>A0A8S1XBT7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1190033</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000119">
        <property type="protein sequence ID" value="CAD8198504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="290755at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398:SF0">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8198504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="344" mass="41381" checksum="A371799430633331" modified="2022-10-12" version="1">MFDTFFMKQNEIIRYQKCRIQIQYKFLQNFIILIKNLHKIIHSFXEDYLIFHSLILDNKYTNYQRSYIKLLTILSKIGGLWQLIFITFGIISKPFILTEMKINLANKLFRFQQEENDLTQSIKKEGSMKQNLEKYLQKSQQQLPNTISNILLFLFGCNKAKIKQLNQANKKIQENLDIVEIMRRFQEFDNFKQIILTVNQRILFDLIPIPLITSFHQNQIDSDQFKNQHHSKDNFERFVEAYKSFFLIRDMTEIPENIGQKLIKFLDQDMIKLFYEYHLDSEQRQVFQLQSEERKSKIIVDRNYLDSESKLSLSPCNSKSQEVQAVKIFLNNQQIKRKFENLQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XTA7</accession>
    <name>A0A8S1XTA7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T1360094</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000136">
        <property type="protein sequence ID" value="CAD8204213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XTA7"/>
    <dbReference type="OrthoDB" id="1302410at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16454">
        <property type="entry name" value="RING-H2_PA-TM-RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045191">
        <property type="entry name" value="MBR1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22937:SF65">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ARK2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22937">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF165"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="236"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="48"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8204213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="284" mass="33788" checksum="D6FA923748D2AC31" modified="2022-10-12" version="1">MQQSKQFTIRKSTVVQKPKLEAIQQKPVCKILQQTTVQQSQLLENNSNVFNRDELKKLQNENEQLKQLLNNKFQEYDKKITIMSELTKQLENELKKKEDDIDYFVHRIDIKDKEIKLQKELIIKLKNKKKDYKKKFLESQQSNQQQQQQDPFQQLLEMRALLLQFRLTAQVLPILQQQSRLRQYQNQFIDVDNMNYEQLLQLGEQIGTVDNGIAREDIRRIRKRVIQASDNIQGVCPVCQCNMEIGEKYRKLGCNHYYHAKCIKIWLLQHNNCPVCKQTVVIAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XTD8</accession>
    <name>A0A8S1XTD8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EML-like second beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1380097</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000138">
        <property type="protein sequence ID" value="CAD8204796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XTD8"/>
    <dbReference type="OrthoDB" id="674604at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002141">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="493"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="535"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="577"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="618"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="660"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="702"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="744"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="753"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="786"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="828"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="870"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="912"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="954"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="996"/>
            <end position="1037"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8204796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1119" mass="126342" checksum="EEEFDAEC1CB1FE27" modified="2022-10-12" version="1">MVETPQMMEILVQVLPEMMVKATQIVNLKQNFFKNFSKMIKEYFTSQFMIHMYKWQQNKQLKNKIEIELQLDTFVNQDENSEVTQIDIQNLEKIDYHQVSLEVWNKMENNQIPLQLQISYDFGELDLNLLKIIEPNLLLPNTLFNKVKIQKERIIQVVSDALKEYNLTSYDFYSEFIKYYHLKQIEKQQNLGKSINIDSFLHDLKQYSIKLAKSMSNKQVTQVQYKQKGLLYKEEGEEGKWLNEFFNDDTQYGSYKKDIRSCSLIQKKGANFLFVHKSIQDFLIAADLYEMLALSTDLDLQILSSIIERLSKEQTQNQDYLEFISNLNLSELNFKFDTLSLFERQHQLNDIQKNIKLLLNLIRILKQHDFNQINYSTNFYTETRRYLRQKISKELVIIEFLKLIVNLTKIDKNFIQCGSNSLNLLVEMKVDLTKQCFSNIKIKNTSIIGANFAKCNLSGSELENVNINGINLNCAQLFNCKWKHLKIYEVHQFNGHNDTVCSVSFSPDGTKLASGSDDISICLWDVKTGKLKAKLEGHTNWVQSVCFSPDGTTLASGSQDQSIRLWDIETGEQKAKLDGHTHYVYSICFSPDGTTLASGSFDNTIRLWEFKTGKQKAILQCNSQVNSVSISPNGTTLASGSADNSIFFWDVKTGQLKSKLNGHTNQVRSVCFSPDGISLSSGSDDKSIRLWDVKTGQQIVKLDGHINQVKTVCFSLDGTKLASGSTDNSIRLWDVMTGQQKFQLEGHDGIVCSVCFSPDGTILASGSDDQSIRLWDVKTGQQKASFDGHTHNVRSVCFSPDGTTLASGSRDKSIRLWDVKTGQQKAKLDGHINWVQSVCYSPDGATLASGCVDNSIRLWDVNTRETKVRLEGHQGYLNLVSFSPDGTILASCSSFDSIGLWDVGTGQQIAKLGGHTYCVQSLCFSPDGTTLAYGSEDKSIRLKDVKTGQQQFKLDGHSDYVRSVCFSPDGTTLASGSVDNSIRLWDVKTGQQKANLMGHLNDVNTVSFSPDGTTLASGSDDNSIRIWDVKTVKEVKTTDMIQKEILNNLLPEGSIYNLNLASSHSTILLISKQPIFQANGALILKGQFENQSSIDLKTLFKQKGSCILERQQQQIQKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XU33</accession>
    <name>A0A8S1XU33_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1390001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000139">
        <property type="protein sequence ID" value="CAD8204839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XU33"/>
    <dbReference type="OrthoDB" id="322631at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8204839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="126" mass="15608" checksum="60A9215D76160869" modified="2022-10-12" version="1">MYFHQERNRLTQHSYYDHLIWLFIILFRHPIDEQSYHYYQKLMYFHQERNRLTLANDHNHLIWLTDRLIITTRSQYISIRKETDRQETITMTIQFGFLLSSLDIPQTNRVIITTRCQCVSIRRETD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XW38</accession>
    <name>A0A8S1XW38_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1380052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000138">
        <property type="protein sequence ID" value="CAD8204708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XW38"/>
    <dbReference type="OrthoDB" id="305043at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15592">
        <property type="entry name" value="MATRIN 3/NUCLEAR PROTEIN 220-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13893">
        <property type="entry name" value="RRM_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="233"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8204708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="457" mass="51853" checksum="CECAD2D435FC9F36" modified="2022-10-12" version="1">MNKNSPSPSSLTPVILVLVTNKQGKVLEHSQFFRTFSSFGQVKKILIFERKIIWKVFIEYENAQQAQSALVLDGTLFDYQLKMRVHLSQRDSLVFQNNNNCGVDYTTLQSKQQFFQQLKEDSGSLNQQLQQNLDMLNQLIAQLSQTTDLKEQISLIQQIQEYRNQTQQLTVLYQQQLRNYASVLQREGKKQKQIVSTVTTPSPSPFLHSTLDSIRQQIPEESSEDDKEEFSDGESISSQSSDKSQKSTHSEDNYNEVFCNDSTTPFSCGASPFTPYPQKVITSPFIMTPTTLPTYLIVQHSNINLRVVYNIFSTFTRVDAIYQSHHGAFLQLASKEEAIRMKNLLGKALLFGVPMNLIVTDQLSLDARQVTLPPNERKPLLQQQLSNSILITGLNGITIEQIYQYFGCITPILNMKFINQSSCKILYSDVGCSLSVLGYIQDAKINGRSVQLSFTAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XWD8</accession>
    <name>A0A8S1XWD8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1380079</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000138">
        <property type="protein sequence ID" value="CAD8204762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XWD8"/>
    <dbReference type="OrthoDB" id="301227at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="30"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="144"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="73"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="109"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8204762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="554" mass="66151" checksum="D6E188524D7780FC" modified="2022-10-12" version="1">MDNQFYNQDSLAFGSPQPISQLQPSKAIKFENVPQKQNKIHSNVKFKEASKKVQQQKIDSVQKIRPSSAQQQEKFKPSWNDKVESKPKQFDNLKKNEIFKIETKLQKQQIEREKENKRRQLENPPPAAVKSYATIKDIEQVINDQQYNKDVSEMKKQKKELEQLQWMVDQEKKLKAEQNKQFEEKLKQLKTQQHPDLAQDIMNAKKKGIENKESPYKTKEQKKQVQKKQLEKNVDDIYIEPQQNNYPIWKDGINLIHYEAKKPLLPGEYDQNDLDLIPQKQPQQQPQQQVRIDDNKLKNELIEIQKRELQRQHELDALIDKHQQLLRSEAQNPNKYYKEINEKLDKIKGKVDPLVKKIDSVQIEYPFTGHLLARGVSKMIQVHSERLTELLIDDLLFEMVDILDTLEMKKKEEERQRYEQMALCDYIEAVKDIALDQDKVEQNFQQTRQQFMRDTRLDQYVPVEQLGRDQQYYVNPMDRLRSIELSSGFIIKIMEDRKKRIQYITQDKQYYPKNLEQFDLITETILEEIIWERAAALYQSSDEMAEQIFINEFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XYB2</accession>
    <name>A0A8S1XYB2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1400132</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000140">
        <property type="protein sequence ID" value="CAD8205398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="77931at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2486"/>
            <end position="2506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2599"/>
            <end position="2623"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2635"/>
            <end position="2661"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2681"/>
            <end position="2704"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2794"/>
            <end position="2810"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2822"/>
            <end position="2842"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8205398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2921" mass="341749" checksum="63B4F10EC36003F9" modified="2022-10-12" version="1">MFQIFYILKLVQSTCPYSNFDQFQLNSNNFYQEKNYERGNTYAFGVWTQFAPIRKNSYSDKSKPYFDSTRNLDGFHILNFQNEGNIQTIFYQQPLYVTGYPTLIILQNTQSGVLAYRIQEALFYYEGYWIFTYFSFNYDNRYNFASYSTGDDRFFNFLVTNRRLKPQTNNVKLEYGGQGLYNIDTSSFNLNIFLGRISIITIFENPISFFNNQDKVKFVQFISKCSVPQGCQNVVELLLFNEPYNQYNCTIRNYQVDIHGLVYMFDFWIKRDSYKINSENEILLSFGGLFSTLERYYQLALYWRIVNYDVILLHHLDVYYLVPGEQITQYFDNYLVQLFKPIKSYEQWTYLKYQFQKIGMYQYVYLSIYQSCCDTWEQYYVLNVPYYQQYTNYQATITLFREQELGTIIPFEGLISNLRFKYCNIIDYQFGQESNLNCNTLCKTCIGPTKYDCASCYDEQNRQLQSDLHQCLCKSNYQESDSLICQKTLLNSISQQKELYKEYNIIEVQYNKKCKYGYFEVWKNLKFIGCIKCPYFGQTHNSLNCINCILNSHNWYLEPVCNIDYSTDDSVSYYNQQLDYQTQEIFIINFDLMIIELCFGCEKITETKNSFSIIKYVNGIYTFYQCKQFLYPNNNICELCEMNCIKCKDTEKCIECNQGFYIKNDHCYQCPQGCLQCILDSRLDQVKCTDCQQGYSLVNDNCIKCGYLCEICQQKINRDTGQEYMKCLKCNYNTYISLDGESCYVNNLNHCIYPFQFSIVSNSINTILYDFQPQEIQYIKTGCIFCSTISYLTAENNQFTCTPQGSDQIKSGCYQTLHYSGYIDCSIGINNLMALGFGYCTQFIPHCTICFRLDWEYANICYICEDGYYNDYYSSFCKPCNQECATCIQIDSSKIVKSSIIKIFSMLNDFQGTKHYSQLTQNPNKNEIEIICTKCSDGYIKHFNKCIQKCPNNCKECKIINDQQVCISCIQFNDLQYHVYNGRCIQCPLNCKICHPRTDDEKLKFNPYFNNKNLEYFSHQCISPLSDDLYYDQDIQQFIKCDNIIECQKQIIIEIHVYCNQIDLDNYINQLNDQQKVIQSKFHILLENLNLTLYEYYDYFLYLNQNFIKIVTYQITIVNNQICSLNKQLNIQSNLQTNCFNLIQLNLIINGSGNTLYLNNHLQLINFKYIRIEKINIQISNGDISMDNIYGYIVEFETINIYQDLTQITSQNYLIQINQSIQINISNLQIHNINVHFQNGLFLITNQNLDIKLIVVDLIINDAKIINTSIIKFNTSQKIQTQIQNLHIQNSQFTNTTLIEQLQQTKIINIDNFIIQNSIIVNTTSFLNFKLIQKVEFKGLQFQDSILQNSNFISSSKILLLIEFNFLKLQFLEQSTLLINQDSNFLEFIQIENGKIIDNVYSNKHALILLLNSEIVYLKNIQLINNAINIDNQKDSFVKDIELSIINIQSNIVIFDTLIIQRGIGYSEFTIKNTNTFKLMNTVITLHQNYYFQSLFNNNQECKLHPFNQFMYNNLMNLQNSSYIQISNTNITDIILYDSVLFEIQILSQINSQFIMINCNFSSNILHQLNKYQKVSLLSINSNSYLNLDINNITFSQNLLNLLRESNPQKQSLILIVEAFQAKSTISNCLIEKNMNLNGQGSILYIDSDLIIVENTKFLSNSAFVYDTLKQFLRWGLTEDIFLEHLQKLYITQSNGGNGNFQARQIIFNQILLQSSISKQGAGFVFKMIEDGNIQISNSNFENLEAELMESSQGGAFHISSQSLYLSINIINTKFLDISSRKGGSIFYIIPSTKLFKITLLNSTFNNIFSIQGGIIYVEKSIHQVLIFKMRGCQLSQNLDKFNKFINSLSDVSNNELNTLTQEYSLIYCYDCQFQISDLCITNYLPIPLFQFYGSTNLKNISIQNIIFSNPLIQFSPVRESDQIILHNIEFQNCTQITITEINIPQKQQEKIINNNVRTSCNQSLIDAPLFIMSFNESFSNINQITLLDTLFIQIQSSTMSMILFKFADYYQKHQIQLSQINLQNNKCNKCSNGLLNFQNSKQISIKITKITLSNSYCGNNGCISFIQLDKRILQSLDSKYYIDNSFIRIEDLQCINNTAQYGGCLFIQNQIISISQSLIRGNKAVYGGAIFTKGINSTMISENVIITNNSAQFGSAIYNQDNLKRNVKGIELIDNYGLNQIDEQPQQIYLQIFKDQIIKPTIVSSSKNHIKSQIIGKDGQISIIHIPTGIPLKVYRKFEIEKNSYNQKIIEMRLYAINSQLEIVRNLTNTYCELKINNQFGQVQNLFLNKNKIYFNQSTFSYNLDDLIFYIPSDSNQTFEFSIKCNSIYVPILNNTNNLIEGYHQNYTLSLLIKPNECQLGEYSQSKEDYCHQCLVNQKQYSVIVGATNCQTVDDQKIQEITQAQIFLKKGYWRMKVTTSIIDFCLNRQENCIGGWGVGNDLCQQGYIGALCEQCDYYNIRGGGYYQRESFFECQICEKDDLHQIINFAITILIFICLLVLVNYLSKKRVQFIRLNVKEFRVPFRQGFFKQKEVLMGQSIKIILFYFQIISYTQEISSVFSDEIQQLIHFFGDPFTLLGIYKECQLLNLEVNVIYISLLQNFICILILITTFVCICFARIFIQKLKITQMEFFNFVYLIYIFIVGSFIRSIIQLIAFRNIQDVYWIHKNISYNYTDKDHLRLVLFLFLPIVIGLGIVLPYLLLQKLKSSLFLSKRIPFKDFGFWFTSYQNDHQHWEFYKFLLVNGLICIIIMIDANDTIKGVLSYSLLIIYTKVCQDSKIYYHAQMNRFESQLNIISIIILIFCTLISIQEYQGSILKNIILNFINSLFILGGIFIILHLLKLNSQYIKRIFCRLFENLGIFNEIFIKYNIFQAHSKTSIEIRKKRFEQLRVFLKQQSKTQLLIKKEYQISEEQNETQRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XZU5</accession>
    <name>A0A8S1XZU5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">COMM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1440087</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000144">
        <property type="protein sequence ID" value="CAD8206545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XZU5"/>
    <dbReference type="OrthoDB" id="286030at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017920">
        <property type="entry name" value="COMM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07258">
        <property type="entry name" value="COMM_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51269">
        <property type="entry name" value="COMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="COMM" evidence="1">
        <location>
            <begin position="87"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8206545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="157" mass="18915" checksum="78EC18E414D138AC" modified="2022-10-12" version="1">MKYTDNQEEITLDQLKEYLKDQGNYNEETFSQNVDIFQQLIDDLIQNDSEQTYEQYGISSQELHQTWLSEKDRVLSYYIKKRTQGQKIEKFQWSVNNQFYSKKAKNINKPVCIMNLKSKDVVNKTYKFHTFEINEEDLNYIEDQMKKIEQTINALLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y1J8</accession>
    <name>A0A8S1Y1J8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1460109</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000146">
        <property type="protein sequence ID" value="CAD8207215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y1J8"/>
    <dbReference type="OrthoDB" id="10352706at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8207215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="111" mass="12743" checksum="64EFA2EBF5A50217" modified="2022-10-12" version="1">MTTIPDSSCSNVIDYKGFNVQITNYGKSVLIVKGNQEFKVILNEQDASKENQQYIKIQPEDIIQDEQSKLNYVCDSKKNIKTKVLCFQKQQGIAIRWSTGLQSTFIFKIDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y346</accession>
    <name>A0A8S1Y346_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Importin subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1430042</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the importin alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y346"/>
    <dbReference type="OrthoDB" id="295494at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001016">
        <property type="entry name" value="Importin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024931">
        <property type="entry name" value="Importin_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005673">
        <property type="entry name" value="Importin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8206124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="463" mass="52723" checksum="7F51AD4EC094E0BD" modified="2022-10-12" version="1">MKNSQKLGGIKKLQQNCNHEDLVQQEQICQELKDEIIQILRMPQDHISAKDAIIALKSENFIESHKAIIKLQRTLSNFEQAESIAQENELIPILFHMIQFGCTYLIKLEAAQIISILAGGRSEITKIIMQNGILQLSLSVLDGNQSELIMLIITILGKLAGESVKYRDSILQVFTMEKIISKMEQKYKSIYIWCLANLCIGRPSPRFDKVKLAFEIFAKVIMEEFNQQNLKMINDAIWALGYMIDGEPNRITALINSGVVPQLINLLPMGNFVSILRIFECIFYGTEEQIKYLLECGFIAHIRMIVDPKSREKSGDMIQTFAATETLFKVLSNQNFLISLFRSLSDDNSNLRGDALEVIGNAIQHGTNDDVNQLVKNGLIQYLLKMLDIANEKEIISIHLKKGLEMLIKIIRKGEIKVKDNKSNQFFTLFVQLDGISIIQKLLSYKQDDVAKYALIFKQEFAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y7B9</accession>
    <name>A0A8S1Y7B9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein uL6 alpha-beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1520074</name>
        <name evidence="7" type="ORF">PPENT_87.1.T1520076</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000152">
        <property type="protein sequence ID" value="CAD8208867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000152">
        <property type="protein sequence ID" value="CAD8208871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y7B9"/>
    <dbReference type="OrthoDB" id="291967at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000008">
        <property type="entry name" value="50S ribosomal protein L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000004">
        <property type="entry name" value="60S ribosomal protein L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_uL6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_uL6_a/b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002359">
        <property type="entry name" value="Ribosomal_uL6_CS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655:SF16">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="60S/50S RIBOSOMAL PROTEIN L6/L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002162">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00700">
        <property type="entry name" value="RIBOSOMAL_L6_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="5">
        <location>
            <begin position="12"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="5">
        <location>
            <begin position="118"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8208867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8208871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="196" mass="22402" checksum="9A5AB2E626A7C52C" modified="2022-10-12" version="1">MKYILTEEHVTIPEKIEITTKSKQVKVKGPRGELIKNFRHAALDIQVQKKVNKKSNTTKSRVSVRMWQSYRKQRCQVNSVASSIKNMIRGVTTGYKFKMVLAYAHFPIIINLLEKGMGVEIKNFLGEKIIRTIKCLPGVTITRNETEEKNVLTLQGNDLNNVSLTCALIHQACAVKNKDIRQFLDGIYVSEKRLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YGV3</accession>
    <name>A0A8S1YGV3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1660018</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000166">
        <property type="protein sequence ID" value="CAD8211967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YGV3"/>
    <dbReference type="OrthoDB" id="289494at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8211967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="124" mass="14587" checksum="79D5A8D20B2062A9" modified="2022-10-12" version="1">MSLIQNDENFISKRMAVAIAILILYLLHYTTLFLSSCFNYKYQGEYTSRRIFLYGISGLLSMSAIQSNIFSAVLAWLTLIPLSILHFSNYQKFKTLYKIQDSLWLTYYTLQITWYLGIIIDFYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RW13</accession>
    <name>A0A8S1RW13_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0010521</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RW13"/>
    <dbReference type="OrthoDB" id="436852at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15489">
        <property type="entry name" value="PHD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46364">
        <property type="entry name" value="OS08G0421900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01426">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BAH" evidence="6">
        <location>
            <begin position="76"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="5">
        <location>
            <begin position="197"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8132258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="246" mass="29117" checksum="184E28CE0DA589F0" modified="2022-10-12" version="1">MSAPNINHETDRKLNEIFNSIKLENVKTQETLKKISNTNNLITQVRRNQSLALLLQNVQNLNQTHKEFKEIKINNIPIKLNSNVVIYTKKNGHCIAKTIKIIGIVKFQHYTPIIQVQWYQKKKDLKQIIGQYFDCISERELFLSDQYDYIQPDIIVGEASVLELEQFEQKNQSTGFIFFCRSFYKNHQILPPIQKWEKHCKCRQPKNPDKKHLICDICQQWYHQDCMALNYNIQGIYVCPSCKKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S0F2</accession>
    <name>A0A8S1S0F2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0030454</name>
        <name evidence="2" type="ORF">PPENT_87.1.T0030455</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S0F2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8134711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8134712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="131" mass="15923" checksum="4C1FD66372AF8756" modified="2022-10-12" version="1">MDLIIPYLFLNIKTFQLQYYNYFVDMQLINFFQKVLSLIQIDTIIEQLWAAKYKEICDLYRNLLNLLKLLCKLQIQKKIAENPIIPNFKENQIFSEVKTKCRHYKICKIHKYQYNYEGFCQDKTEKSGIYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S240</accession>
    <name>A0A8S1S240_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0020431</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S240"/>
    <dbReference type="OrthoDB" id="305048at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8133490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="264" mass="30694" checksum="76FFFFEAE3611296" modified="2022-10-12" version="1">MGAQCCNNQQYQEDVEPCDDTELNRLDARQINIKYNYKKKQKEMNSSQTTKTQERTKQLTVTLKSGAEYLGDWVGNKREGYGILKWPDGSEYEGEWKNNRAHGQDYYEGQWENDKQNGRGIFQSISGCKFEGQWKDDLQQGYGTEIWEDGSKYEGYFYEGIKQGQGTYIWNDGSTYTGFWINNKRHGQGSQVWKNGKEYNGEWFEDFMCGQGQIKWPNGYCYVGLFNKDVPNGYGILKWSNGKTYEGGFKQSKANGKEQVWRMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S288</accession>
    <name>A0A8S1S288_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0020560</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S288"/>
    <dbReference type="OrthoDB" id="296584at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="257"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8133693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="284" mass="33242" checksum="3DAD93D95F39DE14" modified="2022-10-12" version="1">MEQYDKYQLFLRLTNKKEINNNYEALNSSCKKQHPTVLNGKRNPNNEAYKKLHQSSEIFKENTTPIKNDVTKPKNYATERKSLEDSNTKRTLIPSLDQFQSPERRCSSNIRYNANAESSQKDSKRILKTCEQRQQDFSHRINLPISAMQIKDYKEDNYGLGKYKSHESTQKLKSYLDLQGHLNNQSFQSQTDRLFKSQTKIQKNILSSYQICKAQSDIKNGKKRFIQSAMSQQLSPPENPIKPKNINKVFIKSSQEVWDNQQSQQKAPVKMKHAYQQKSQIDLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S2P1</accession>
    <name>A0A8S1S2P1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PPENT_87.1.T0040344</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S2P1"/>
    <dbReference type="OrthoDB" id="290094at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000315">
        <property type="entry name" value="Calcium-dependent protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 15 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PH" evidence="19">
        <location>
            <begin position="32"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="137"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="522"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="522"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="262"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15 17">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="266"/>
            <end position="267"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="16">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAD8135671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="557" mass="65115" checksum="53019A98B9E337CF" modified="2022-10-12" version="1">MQIEQLFFDSSNQNDKNQWVKLDSIKRSISNIRLFEGTLFIKSKKQNAFDPKFFKLFSDRLNIYKNGREQKETAALFLTNVYLDLKLLVEYEKDKYPIILINGNRKLILNARSQESQIKWIEQFKKTCIMNNYKDIYTNIKVLGKGTFAKVFLGHKIINKSKFAVKTFQKSALIDKKNTQRQGLINEINILRSCDHPNIIKLYEIYESEDYIYLVMELLQGGELFDIILETPFFLESKIALIMFKIFDALEYLHTKNIMHRDIKLENILLKDKSENFDLKIADFGLASYTESELLIKRCGTPGYVAPEILDDQKYNEKVDVFSAGIILYILLTGQAPFYGNSLDDVIEKNRVCQINFQGLKISQDAQDLLYKTLEPNPKNRISSLEALSHPFISRLYKRQVNSMDDIPLDELPNDNYSAFENMKKFCQNNMRFKMSKLKKNELIQQTPFMGAREYDPVKATSDSWLNEKSNEIQSKQIFNFPCIQQQSSDFDISILNTPESRKDTQRFVQLNALQQIAFNQQSPNQSMYSPNNYQKQQIQMNSKKASQVRDQLKKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S744</accession>
    <name>A0A8S1S744_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0040163</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S744"/>
    <dbReference type="OrthoDB" id="296267at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8135317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="306" mass="36504" checksum="1787DFF985725517" modified="2022-10-12" version="1">MYQPKKSQHNQVSKDWQTIWKLRETDFISHFLVSKSGNLVAFVIEGKKLYVISLLTKKIIFELTFVKFQLYDLQFTSDEKKICIAQEESKSLRIIELSTRSEICYKLPQSFLPLDSYILVEQEKKIYLYQNDALKLIDLNQENLELILQFDGEYRVLQKTSMDFIINIAFQNQLQVMYLKNNFCKQKHKYIESNKIIHFTESNQLLSIITYENNYQLFLKNLQCKKLIRKFSDKVLQNICDIKYSQNRKYLFLNTGNSLIRWNITTSKISKLQVQNNVFEKMWIEGKNEIYILNSNNHIQKASCDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SA63</accession>
    <name>A0A8S1SA63_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0040430</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SA63"/>
    <dbReference type="OrthoDB" id="302883at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="207"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="133"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="221"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8135804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="293" mass="35011" checksum="6CAE47A290234297" modified="2022-10-12" version="1">MDSKENYFNIKQMTESFRNVKKYSQHSNTILDMFIEITQKLDKSSKDYLSKVIPIDDDKSTLSQVDSIIIMNYFKQIVIYLIKTKVELEEKTQQIESQTQYESLIQKLEADVRQHIRIEQQLRVYTETLQQKIDDFVIEREQQMQLIQEYEQQIKQKNKDIVIYQSELRKAVNQNQQRHTKLITEPILEEYDTKYHMNKLNQTNYTHQRKASQVNDDKSVSNKSQINSYKNSPERIPIRKISVTNYAETKTIKQPVFIRQPCPMSYSHLKTEAQISYRSSSQESKYLQKNQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SBU4</accession>
    <name>A0A8S1SBU4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-CH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0060201</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SBU4"/>
    <dbReference type="OrthoDB" id="305001at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8137656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="234" mass="27111" checksum="33F47CEB97A0C0D1" modified="2022-10-12" version="1">MDTTTAHNQPQIETFESKLITIQGTSSLPNEHVYRIRKTLHPQTTANQFHLSKTLNSSVNSVNQLCHICNKHTSASTTKYCACQDRQYHLLCLITHIKLEYKQGNGIMQCPYCNLYYPTVLQTNLSYQNLTKKQLLVYASAFLILLLLIIVEVTIMIAMNLHLPFYLFLSILITIELLILLRLIRKTIMVARIVRFSLKEYQYCDEIIQSQYAENSFRIISALKNNKAEQLFTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SDJ4</accession>
    <name>A0A8S1SDJ4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">phosphatidylinositol N-acetylglucosaminyltransferase</fullName>
            <ecNumber evidence="2">2.4.1.198</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">GlcNAc-PI synthesis protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0060423</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.198" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8138046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SDJ4"/>
    <dbReference type="OrthoDB" id="734129at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000506">
        <property type="term" value="C:glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017176">
        <property type="term" value="F:phosphatidylinositol N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03796">
        <property type="entry name" value="GT4_PIG-A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000148">
        <property type="entry name" value="Phosphatidylinositol N-acetylglucosaminyltransferase subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000093">
        <property type="entry name" value="UDP-GlcNAc:PI a1-6 GlcNAc-transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039507">
        <property type="entry name" value="PIG-A/GPI3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013234">
        <property type="entry name" value="PIGA_GPI_anchor_biosynthesis"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45871">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45871:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08288">
        <property type="entry name" value="PIGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="390"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="PIGA GPI anchor biosynthesis" evidence="9">
        <location>
            <begin position="41"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="8">
        <location>
            <begin position="196"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8138046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="446" mass="51036" checksum="35C05D32C73FDA53" modified="2022-10-12" version="1">MVNICLICDFFYPCLGGVEMHIFQLGLCLIERGHKVIVITHRYQGRSGVRYMTNGLKVYYCPFIPAIQTVVLFTYVGTLPIFRQILLREEIHVVHSHAATSYLGGELLLHAKSMGFKTVFTDHSLFAFNDASSFHVNKILKYILCEIDHSISVSHVSKENLSMRASLDPRNISVIPNAVDCSRFTPNPQKRYPLNTINIVVICRMTFRKGVDLLVDVLQIICKQHPEVYFIIGGDGPKKRILEETIQRYNLQNQTELLGSVPGHQVKDVLNRGHIFLNTSLTEAFCIAIVEAASCGLCVVSTNVGGISEVLPKNMVLYADPNPEDICQKIVQAIPIAKNFQVYQQHELIKKMYSWEQVAERTEKVYYKILQTQNQTILKRFKDCYSNGQIYGLYLMILLILDLIFLRILDFLQPHQTIQKSGIFNKIYKNQKEKFGDHQFKAQQQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SL60</accession>
    <name>A0A8S1SL60_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0070478</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8139114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SL60"/>
    <dbReference type="OrthoDB" id="6622877at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="636"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="35"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="141"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="406"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="649"/>
            <end position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8139114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="661" mass="78589" checksum="1F536F05E5D81171" modified="2022-10-12" version="1">MSIHGKNSGIQIAQHQSPARVIKEVSDISKEFTARKVLERRISELELKLKQQENVIQKYQCQLDEASQETYKIGRQFEDALNDVRQMRLLGEKKDQQIGILLEENDKVVQLLEMQRNNQQMDMIDTIQKLEQQVRDRFICEKRLNDEIQSYKIKLHQVEDQLKDRNHLIEDLRDKFTNLEKQCSTDDQLGVLENKRGKEIEILTLQNGDLQLKIQNLTSKIQLLLEENGNLQKTIGNQNNEVKFQQEDKFQKMIKIINENHQKEIQKLKEQNKLELQEKIEIIENLKTNQNLNNQNNIQVDDLKSAKNTISNLQSESLQGEVDDIHLQYEKLLKKNNQLNEQNFLLQQRIRKDEINNKEKISELEQTNVKLLEIQQINETKIEELQQKLKQLPTRVREKSMDDLKLRVEQTKLTEQDNKIKKLQEKIDLLNIEIKEKNQKINQLQEQIKQAIYEKDNAIQQIKLECAQEVKQVQDQMKMELFNQQKQFNENQRPYQEQMKSSQIEQNKLKSQAQRYQNEIKALENKIANLLIEKEQINNQMEKIKVTVENLKKDKQFQELKIQENLREIESWKEKYVKSVSNCEKQNERAQKEQSKAEGYKEEINKLNEQLEDIIEQNNNLEEQLQKAQLESRNLRNQLMDGVRRESGLENQSNGFNKPNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SP30</accession>
    <name>A0A8S1SP30_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FYVE-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0080514</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8140224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2352506at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00065">
        <property type="entry name" value="FYVE_like_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="7">
        <location>
            <begin position="17"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1644"/>
            <end position="1667"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="384"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="481"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="760"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1044"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1672"/>
            <end position="1699"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8140224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1935" mass="228653" checksum="DB22795C7ABD3221" modified="2022-10-12" version="1">MKKNQSLFKRVLISGERGKYKACQICQKKFGKEHQCKRCKRAVCEKCSNHKGRVFTESGQEEKALHRQCNICKEESDSIKKFVDTNHIQFLQDTKALEWLSQFGVTKEQAKTEYLQAQSKISQSEQSEIRKLKNNLNIAFAEIPGVFNYSLREFIYHVLKDNEYETLKQVTGRVLETFLSNYNEVGFSPDLIMLTVFFLCFGSESAAFSLLNIFFAEMVQANLYPSNIMQYDNGEDIDRVLLVLNSSFKMQSQDKPLITNFLKSRLIKYQQCFTINMLQFESTYYIIDSLYSLHQQAQEEFIKFIASALSQNIADVKANQLNIEEIEVILLRQVKYSMIQEGYFKTKSVQFSPYTKTMSRSQITSRFSSVIQDQTSSNMDFDRVSILNKIVENQKQEIAKLNQQLNGTESVRKKTVQLKQKDQTNLQNDLKQVEDEDLVFSNLRRKTLHLLNQEEADEDVAVLTDYINKLEEVLILKHRVVKDQNIKILEMQEKNEKLFEEIKKLKKDVQLSEGKFIDQTKITHQIEKEMNEKSRIIDEYKKKNKELQDNLVSSQTENNKYKIQLLEAKALEGGQASGDGLKSNSYLREMQKLQDENAKLLKKISDLENQVLEQNSKSSDQNETRKLQIQITQLEQQKFLINAQLSQRESTITQLQRQIQGLLEESSMKSEGDQFIQERKKNGELSLQISNYQNQEKVWKSKLSDIEHKLKEQVLINQELKTKVNSQNPIITITKDENERSYERIREQLQFELEAKTTLTKQLFQQIEELSIKLNQERTKSQNLQIDYDQLIEQTNFRQNRSQTIQQQNQQKDQRIQDFSAQIEQLQLEQSKSKSQLTQLSTMHQNLQDNFRAAEMKIVELEHQKRMYDRTIQEKVELVTSYETKIKELNTQKNQLDDQLTESLKKQSEYRSQLDSLQFQVGNKGREYESLKQKIEEQELIILGQKEKIHKVKESNEAEQNEKSILGTEQENLKRQLQMKEEQIRSRQEQVSSLNEQIRNLNEKVYNLEQQRTLSLKQVKHLEDQLNSREREYMTEISKVISERDLQKQRVIEAEAQILKLQQNDKKLRNEISNLNEELQQQLLTLEQVNKQIKDYEIQQNQSRESNLENNKVQQLKWDRLEQQNQNMNIKIEAQNQEIKSYTAKIEEQLTTITELKYKVQEEESLRRAKEKQANDKVQQISQLENQLIKIQEKYNQSNRLSQDLQNQYDDLKKRYDVELNSMQSLQSKSIEYSSLQQRYMEQSQKLSSLEKQLLDYNEISDQHQKQKKELQSLTDLLDQKLATIVTYETKIIDLTNQLKESNSQLQQVRKENHEIMQKRAQDMQAMAQLKQIEVENGELKQQVYSHEATIQNQEALLSVLKGNQLNLEQSQMKLRIDYQHLEEKYNEKMIDFDEKSKALFNLQNKFDSLNFRAQQNEENLRLVEDQRDDYQSRYELALQELDIYKGKDKEINLMATNKIELQEQMHQVQKKNKVLERELQVSQNALQSKESDILQLQHTIQKKEQQITTLEGAIVKLKADLNNSKNSYEQLQLELTEMNSEQTSSGELFSQVKKLTNDNLNKNTQIDQLKLQINELQDKNRNLEKQYNKLRSDALVGNSINSDSFELKSRLDELEGQFKQSQFIIKQKEQQIQELKQLLDNQKQQPYQKEKEKQQNNKSKNVSNDHENMKIQEYQFIIEQQTEEIENMKKNSHGLGEELTELLTQIVSVFLKKKIKKDQLNIATVLDQIKLFQKENKSLTIREEDDEQLILQKLQQYRGDYINFRFRIEQILTRITVLKQQMLQVISEAKIQGKKKDVDKNMDVENLVMYFKLLLDSQAVQYSNQLQLCQQYEAELKSYQNSTSPQGQVIQQVKELLSKAPFLTQQQIYQFTEPKVSIKLRKDQDQEDARLLLEARNQQIQWLKNLINEKQDQLAMMNTYLTNLKQLMDKYHLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SR83</accession>
    <name>A0A8S1SR83_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fatty acid desaturase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0100372</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SR83"/>
    <dbReference type="OrthoDB" id="1461976at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03507">
        <property type="entry name" value="Delta12-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012171">
        <property type="entry name" value="Fatty_acid_desaturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32100">
        <property type="entry name" value="OMEGA-6 FATTY ACID DESATURASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid desaturase" evidence="2">
        <location>
            <begin position="60"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8141916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="358" mass="40693" checksum="A1DF5D158B2FBE18" modified="2022-10-12" version="1">MSKGQEQINLAQIRKAIPESLFKKSESRFLVSVAQAISTTALLAYIGYRFIPLTWYALPIWIVFDFLLGTVAMGIWVLGHECGHSAFSDNKLWNDILGYFLHEILLVPYFSWQHSHALHHAKTNHLTEGETFCPVVLNSKMGKIYQKTKDIIGIESFSIVQIFNVTVLGWPLYLLLGVTGGPSRGFTSHFIVPNKLFPSRMLLKVSLSNIGLCFVIFGLYKWAQATSFAEVMALYIGPYLVVNMWLTIITFLQHTDINVPHYDSTSWNWLKGALSTIDRNYPAWIDSLHFEIGTTHVLHHVFSELPHYNANEANLYFKKAIGDLYHSDEKPLLTSLYQSASLVGVEHKGDGVWFYSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SSK6</accession>
    <name>A0A8S1SSK6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0110393</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SSK6"/>
    <dbReference type="OrthoDB" id="293697at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd19756">
        <property type="entry name" value="Bbox2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8142900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="399" mass="45787" checksum="1BC39898F17A0460" modified="2022-10-12" version="1">MSEDIKTRPINYLNCTFGDMHEGEQLNFVCLDTTCKEMGLICPVCRSQKHAKHKVIPLKIFLADISINVNGKQNQNSIDQLLTQLDQVRAKLLSSLKETVQKMVLQIKLLEDQINLSHKNTRQRLLEQNQTQMNFPQIFQQILNSQYKNVDQLKQDVRKIIDNVSQQQAGKIEIDFKPQRLFDQYQKASQESIAQFNQLLGQFKSSIYKISKPIEKFTLPLQAQNSTNYTFSSVLKSPSINIIEQKNAHQTTNQNSENRFILIEPQILESCKIGIKITNLSNFIGIGIAYKNVLLGKNMKFDHQNLGHGSYMISSNAHTWSHSKKEENMIQKSFTFTTGDIIIVEINLENKILKFTCKNKPNDNQATILTFDNPDNDDIHFCINMCSSGEKVEILDDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SSR7</accession>
    <name>A0A8S1SSR7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0110155</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SSR7"/>
    <dbReference type="OrthoDB" id="5979581at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001262">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="27"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="364"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8142456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="546" mass="64004" checksum="A0A6E4E436E14ABF" modified="2022-10-12" version="1">MNLKINSYLLKYFQNIKKVGDLVQNRFFISQKIGEGSFGSVFKVNDQNNNNMAFAIKIQSEDDETNLLEREIKVLIELRKYQGFPQIKFYGQERGQTYCIMQMLGQNLEQIFKKLGNVMNLGSVLRLGIQMIERIAIMHQSRFLHRDIKPDNFVIDGPNNPRLLYLIDFGLSKYFINKKGDHIVQVKKAGLIGTARYASVNAHEEMEQSRRDDLESIGYVLIYLATGVLPWMNLQIEKKDLKYAKIKQIKKEIKINELCKSLPKCFEQFMNDVKSLEFKETPNYEKLKSYFENQIEIDNKLYSNNQQPFVYDWERLSDFAKQKKHQTIHVMENQLKNQHIQIVINPHKQNQVAKQELFLNQQIINSQQNSIQNEQNKIDQIINEQKQEQLQIINTTSNQFKTHPFVINVIENQKDQLQPIKLDESRITRLQNHSTTLLQNPHFNSSFQFDSFIQPSIATSRMNNYIESESVVSEGNVLPIWDIKGSQQDFQQGTRILEGIRKPSQIILRKERKIHSQIDTVKQMQNLFVHPNQIIGDNETYVEGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SVI2</accession>
    <name>A0A8S1SVI2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0120208</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SVI2"/>
    <dbReference type="OrthoDB" id="297790at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8143396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="258" mass="30807" checksum="1896028CCC7AEA32" modified="2022-10-12" version="1">MYYQENYRKAHQYLIQRQKQIDFQPNKLNQPQIITIRKILEVIRSKDRSNSYLKYKKTTGQEKKEETHSPQRLITSNQEPKNHNRSIVSKLLRLNKITRNKTQVTPIQYNFIDRIIEPTKKKIEIQSERNSPLRIRKNIPCIDYQMSTQVLVSDNQKQVNNNMTDRQFFIQKPRLIENINQQQAQSKSIQNFLSHHQSIRTEQASPSLRRLNKTSYQCEQTKNNTNQIKNENIDKQIQLIRKVSGWTIQSQDSIQTPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SX52</accession>
    <name>A0A8S1SX52_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0120460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SX52"/>
    <dbReference type="OrthoDB" id="303403at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="472"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="680"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="482"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8143899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="699" mass="81261" checksum="88B21779D45D727A" modified="2022-10-12" version="1">MIKLEAHTFHNKNVMTPAQLSNKSPIDIVRQKKIIFKRELLNTEQNFLVQIEENQEIVYLKPDVPDLIITDLTNMAIQEDIPEYQALEYHNRAAKLLEFYTQNQIQVQYTSVLFSKINYAIILKKYAVQNEALEILLDCDEILKIKGKVLKKNSNESLNLAQIIQNKLLRLRLMFQVTLLFSETKKNKQALDMAKKALRILKSIIQNTIKLCQQITVMSLINKKQRANSEISSPLQKIQSSQSINLPFQQSQILSYPQIVENVFKEILISITSMLPNDEKSNDVITIQNFMIRNNQNRSQSLFASEYVFQPQNNQQNKLFTIIDDNHPMLQMQILGLMQLTYVDLEELFPINSYEMVMAEEVILELIMLTGLSFYSISTELRFINNQSSKLTVEIWLGKAIEIFYTYVPHSSAIFNQIYQVYQKLYGVDKQSIPEDEEVEYHTKLLKPHPFNNKSTLSNKIVIIIKVPNQNKQSQSKDTETKQSQNTQTNQQKIQQSIFIKKQILLPQKHTFTQPNSDKKFDTMQDEKDKSPQLQQLQQQLFIKKNNNSLNSTDVKQRVEILMNQILNQQARLTQEKLKQKNTPITILKQKYQVTSKIQENQNTFNPLMTSQLKTTIPVSRSLSNSRKTSQTNFKNQFNSVKTAQMRYRTQMSSGDESSKQLAQQIAAQLASFNRQSTKAFQLKRSDSAKKQKSQFQQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SZL0</accession>
    <name>A0A8S1SZL0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0130095</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SZL0"/>
    <dbReference type="OrthoDB" id="288412at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8144174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="333" mass="39716" checksum="E0429F3CAFCD03ED" modified="2022-10-12" version="1">MIDYAGIISMKREQFHVNIRKQRNEKQFKESRKKYIQSLFFQQTEMQEMSNTIRDKLEKGENLQGEVLQMMVDTILDVLQKNNNMNLLDTIDAAVWMLKSIEEQTYEDELSDNLKMLRIVDKMILYSQGYEIDNEPLNSQIIIYAAKFLKYWTQMEDKSLYLKFGEIAETIYFLLNKQEQMFIKRGADILYNMALIENGKILNQIHELTIFGQKLVKPICTILKTNHNIKILSTLWRVVLQTFLDQDRNGQLQYVNFNIQENQENISFFNLLINQVIESEQQNLKLFKNQMHLIQQILDYYQSENEQIYEEFKQLLITSQFSIKLKDLCNNCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T1F2</accession>
    <name>A0A8S1T1F2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0140203</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T1F2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="172"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="348"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="348"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="375"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8145359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="422" mass="49080" checksum="130C6BD234C34FAD" modified="2022-10-12" version="1">MINLNEEQKIQQNKLFNIFGQHTKQACIIFIMNALLRVQENDTFFMSITLYLTIIMFLFNFVAAVFLLCFKREFPKIEYFLIYTMNVFMFIILLVYSLNYFIQKKEQDHLLQLYIKTYIMILLGKEIEILIIKIFPLELTNRSVNCFTSCIIVMMILSHGDKYECQNKYSMVLLSLLLSNVLTSIFNIIFTLILFNQNRSQKELRNFADLLLTVLFLVHLAQYVLVFIKTNSIIEEECQCLSFIFQFQKYVAPINFISFLFVIYQEVCYFKEAIKYQELIEKNSQHNNMYSAAIQNQQTLKQGVRHSIKSLSSVVNPMEASQSNSQAPNFGTDKQGQKLEKMGTSQFGASQHNQGMQHSWANVQNPNPFGTIVKEQQDSIENQKRGSKSVDESLIRQEESQIPENLSQVNNPMESGSVVTIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T281</accession>
    <name>A0A8S1T281_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0140325</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T281"/>
    <dbReference type="OrthoDB" id="298677at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13188">
        <property type="entry name" value="PAS_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="5">
        <location>
            <begin position="478"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="6">
        <location>
            <begin position="745"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="365"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="378"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="803"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8145599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="873" mass="101341" checksum="83CB910F371855E1" modified="2022-10-12" version="1">MNSVNLRVVIQLTYFIMVLTIKLVNHNSEYFQVIFSMILTLLFIPFNRYINTCVEQQQLICKSLWQILNLFCLGSVDYGLKNDGLLSRFQYFWLGLTIQLEADTTILNQIARIGYLLVVIASANLTSIYLLIDSSSIGIIFGEALYGLLLLYQFFVVARTQEKTLPQKKPSSRSFTEKLESPQQQIFDEDSNQIKFMSLNQGIVKNPSIYSKDQSVLEQIEQQKTFYEILFNQFPEGIIIINDQNKIDYHNNQVYTLLGRKHKQNRQDDILSRLYELKNYSSKFQFEEQAQFDQFFSKLDKKLKYNQHEYYQPFDQQFDQQINDDNSKESIFNNNFDDNQTDYYYQAELSKAETLKQELDKALQEKNQDKNGLPSPYYKQQSIHSQKTYKGQDKIRFSPGVKKMLCIIEESSEDQNNELQEDKSQDECNPGLLIQITIKPYIYENKRSVLLLIRDVSLFNQFKVLQQQNKNKSRMLSYVSHELRNPLAAIIEINQQLKIQFQQDENLISEYLQPLQSSAVSIQGLANDLLDLAQLKAGKFKLTYQEFSIKQLLNDVISIMQYPVRLRNLNLKQDFDPKLPQIIKSDSQRIQQIIFNLISNATKFTKEGGITVSAKLLQPKIIEISVEDTGVGLKPEDKNKLFQPFGKLEDTKNMNTSGVGLGLMISNVLAQKLSGNDEGLQVDSKGEDKGSRFLFKIQDVSDNQISTQLESKFLIRKESCSINLPIQKKFEDQLIGQTSICRCPQIIIVDDEPLNLQTLGWKLDRMKYIHLKAKSGQECIEILQNWQNNREYCCNWIKFIIIDINMPYLNGYQTSKLIRQMEVEKQIKRCKIIGCSGFTDYESKRVGFENGMDIFISKPVEDKELLSALQQTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T2D1</accession>
    <name>A0A8S1T2D1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tyrosine-protein phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0150372</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T2D1"/>
    <dbReference type="OrthoDB" id="10252009at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46653">
        <property type="entry name" value="SPECIFICITY PROTEIN PHOSPHATASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46653:SF1">
        <property type="entry name" value="SPECIFICITY PROTEIN PHOSPHATASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="2">
        <location>
            <begin position="7"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="253"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="358"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8146493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="415" mass="48106" checksum="C98E83D1979DFF69" modified="2022-10-12" version="1">MNYDYIGAIKIKDGLFLGDQFAAQDLEFIVTNKVSRIINCASKQIPNHWESIGIIYMSFPWMDNDQQVILQQDEIINNIMKFVDDALNNGESVIILSVKGHNRSVATLCVYFMKKYRWTLYKTLQYMHNRRPDLEIRAHFFNQLLSIETRLQKQGYGAKTYNWDEIYTQGENDEIVLRNTYLNSQAQGVAEFKDHDPKPKESKLKFAEKVSMYIPPYDKVVFSKTRSLIQDAKPIIKLSGSSKQIDPSFQQSTQKQQSINIQDQSNQQPQKDSQKQSLQQIQPPQPQRPQSQGTQQQRLTKSDSVKSGQVLDNNVVQPQRVQMNNFMDSREQFLQKSQQQNGPRRPQTAPNQLKAPRVQTTPYKRNTSSGQRQEAGSQPNSQFKPMRQRAQSPNSAYNSQNPYVQRQFKAKAWKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T8D4</accession>
    <name>A0A8S1T8D4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0160104</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8146892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T8D4"/>
    <dbReference type="OrthoDB" id="312646at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="348"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="436"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="468"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="581"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8146892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="615" mass="73155" checksum="38D6FB78CC1F30D6" modified="2022-10-12" version="1">MNNIYITQVKTSAGDNAIINSTERNEKHRQAYTKVLQLKSFNLIQSETRVDTPAKEIDEAELPLREHFYRDSLRRSATFLKVASEEMGRYQTEWHWKFFFEFLFYHIMFYNLFGPFMVIFFWKWPGMPLMINMRFLKHHAQFYFQLLLWLGSLAGGYWYLFDDKNIITLTEVVFTQFALLIRAVVIAAKYATLSEERIKLYKQEVLSEEMFTFDLMMWDWRQQTPKVLFLEQLRFLKRRELDPDFYKIDFLCQPHNKTVKQLIQVDVGFYREWLEQQDESQIAPIIDNTFNCFFIFGYLVNSFQKTHTPGGFSKYVVVQSLILSLTPCLLRVQYFYEIDVGAIDILRIIINVLTTFQAFFGSFVFLQIGLYDLQRKFYILDQCCYMLSIKEQNYSSGKKLLPSINFNNPITLQAWSMLRGMAFDYGRTYDFRIQGFYSLIFIGWIFLFLFGIGVLLDFIHIDFFQTTLLSEMLVILTGFIGYYLWHGAQLNEYYETFDILLEDVRNMYVDILRKKEQYFILNLDITNAIHKKFVFLLKSQTKSLETITQYINLIIEEIDDAIRQLNYDEKHNPFKIYGIRITLNFLQSVVVAVFTVVGFAVQQRMQNVDVACLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TAA2</accession>
    <name>A0A8S1TAA2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0180120</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TAA2"/>
    <dbReference type="OrthoDB" id="308813at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8148547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="239" mass="28078" checksum="B11B6ED7763A4C4C" modified="2022-10-12" version="1">MKINKNIPNYLKLPHLGHQWKFLKNIELNTFTNSSMQQSCKNNNSSLMINSNNICPNRTQYEETPQNNKRRLIKSQSIDTYQQLDQIDSVKESNSGQVKKIAENLIKQYNNKKLTEKQQNNLSIKNQLSLNNQTISKPHLQKCQSLYQLQNETKQNSKQQETNQLNSFNQFLQLIQNQTQSKVKKRQCQVNFFKKSQSMDNIDVVEQQKKITKLLFQSTIRIKPKFSSTINIQDLLQQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TDI0</accession>
    <name>A0A8S1TDI0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0180348</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TDI0"/>
    <dbReference type="OrthoDB" id="3256376at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="10"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8148999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="370" mass="43108" checksum="3AFAC6B7D5F06A12" modified="2022-10-12" version="1">MYQCVLNYTINKSILLGKGGFGSVYLAQNELNQESLQSSQNNYKKLKQLVDRELSIMSTLDNQNIVKMAHSYFDGKNIYIFMEYCNGGNLKKKIRELQSIYPCPKFYQQKYEKLAQEVFWDIIDSLNYLYSKNIIHRDIKLENVLIHNGKYKLSDFGLSKFLSDIDEEPMSSILGTPCYQSPQLLRQQVYSPKTDIWSLGVLLYELLKGGELPFRGFNTQDLLKDIEIKLKTNYIQNQLLSVENKLLQYLVSKMLVIDEESRLSLKDLLLKVNQFRINAQLMQKDNITATETRCKPYRPEKITSKKHSCQNQEIINNKQSIPIRFIQTYKEKKLEATFISKILDDKLSSNILILLALMLFIALMQISIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TG42</accession>
    <name>A0A8S1TG42_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0220331</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8152205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TG42"/>
    <dbReference type="OrthoDB" id="295057at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8152205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="361" mass="43205" checksum="774BD18BA0BEEABC" modified="2022-10-12" version="1">MSVCNKNYLQETFPQYVHDQLNKVAEENGNMDKSQRIFKIIDILSELKILSFGLASKLKTQEGFMLFPEIISEKYIIEFKEIKSCNLRKNESSYFQAWTFANLDLIDMYKLQTFLRILCILAYFECKKYFEVLQIDYKSKYETDLFKVFLLLQTQKQEYQIESDSFFLELIEKCECKNNQFISYMIKFCKEKNMLKCIGYLDSIKSTNKVSSEKRKGFQKFKEQEQMKKESLQESQQISQQIIIQNQNPTQIIKRQSSIFPRKSLDQQCQLIQFQESTKDLIKANGMNRPSLTMRTIKDGINHKYKRENDNRQQRYLQNDTNQQRQFHEEVCRKLEKVQRRKVTCWNSDCLAPETPEIYSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TGT7</accession>
    <name>A0A8S1TGT7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">LITAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0240151</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDIP1/LITAF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TGT7"/>
    <dbReference type="OrthoDB" id="294035at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006629">
        <property type="entry name" value="LITAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037519">
        <property type="entry name" value="LITAF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292:SF6">
        <property type="entry name" value="FI16602P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292">
        <property type="entry name" value="LIPOPOLYSACCHARIDE-INDUCED TUMOR NECROSIS FACTOR-ALPHA FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10601">
        <property type="entry name" value="zf-LITAF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00714">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51837">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="LITAF" evidence="7">
        <location>
            <begin position="64"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8153331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="153" mass="17321" checksum="481C171C3384BB68" modified="2022-10-12" version="1">MSHLAHSQLNESEIQKITYPNLAEVRYEENTEKVFVGIPTQNHYIQSGIPLPTGFLVTPLYNTDSNGTNVPYDAQGSIIPISIQCKFCKRSGTTLMEHRAGPQTWIVAFFIFIFFLPLVFLPFVLKSCKDRVHYCPNCGQCVGKKKFKLCNSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TH19</accession>
    <name>A0A8S1TH19_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0210143</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TH19"/>
    <dbReference type="OrthoDB" id="302575at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="2">
        <location>
            <begin position="77"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8151043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="331" mass="39574" checksum="409336450E0667DC" modified="2022-10-12" version="1">MNFIHYDDDCNIPFDYDDEMIFPPFYQNAETPQHSKSTDLEQKFKNDRKERILKKRIEKKQKTNSDSSNNLSKEELRKLRNRNSAQQSRDKRKQQFDSLMLENQNFQILIKQKEEQIQLLIDENNKQRLKIQYLEEINQNYKCMNCSQEANHEMIRVNVISKQKMMNYGLLSLLAITCILSIVNNDYEFSPKPIALQQISDKKYEFLAPRQQFSNSTSLTLYNHLTMPEHQYNNQTLFYNCTGNEKECQTFLNIIKAENTNNLYFVNDASDHLPAEQDHYLEKGENVYLIKIKQDDEDNFMIFRARCQITESNKLLFERDTYDSYNNSLQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TH26</accession>
    <name>A0A8S1TH26_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0200212</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TH26"/>
    <dbReference type="OrthoDB" id="3268246at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001875">
        <property type="entry name" value="DED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50168">
        <property type="entry name" value="DED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="1">
        <location>
            <begin position="31"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="DED" evidence="2">
        <location>
            <begin position="652"/>
            <end position="733"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8150342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="992" mass="116812" checksum="646A13D484DF151D" modified="2022-10-12" version="1">MLNTEIPNPQKIEEAFNIIMNSKNQQIGKEADTYLRNLEQNYPYILLSLFQIFENSQIYLNKFQALLLIKNVIVRNWVKFQMKDNLRIQNISEELKNHVKDKIVQFLGVVQDDKFKKEINIIISVIAKHDFPIKFQGLVNYFVQGLQTIIQSGSTNCALTYDLICSLKVVQQSVIQNRNTAYRIQQMQFSQAIWNNLLLLWKNITQIQQQELQQNINNYSFVNNISKKLDRLLSLSIMSLNQESDQDRIQLVFILLLEKTAYLLKNVQQQKLLQPNLKTLIHSLGNIQNLFPLIILQGFQDYLMLLKLIIQNDWDDKRVLRTGLIGIIKTLKSLFFLREEHFYNKKLQEAKNEQSRQSILMANQLFNQFFVENMQFLIEQLIKIASIPTDLSDEELIEQEEDLTIDDAKNEMQCPIFTICLICHDQLMLRFPELFIQKITQIMQQLLNSNFNANQCILESFFAILGSTPKITTKLKLQPIQIQPILQYLIQTNTVQSQRRFAFLCRSYSNCFTDTELPQILEYARQLLNNSQDQIVQYQTLMCIKNIITCMGQNFDFRNIQFFEQIAPVIVKLLCNLQKSNILWPLLQLLENLIQKYSETNVDSMQILVKVIENSDIILLMKTKSQLLIGALCDMFFALFVSFPLGSNLSHLYKLALVLIDTNIDSKENNIFELLQFLLQEYEPISDNNSTGVLFSNLYSTHEKLFLEDTDLNHMQTILRVIEELYLLNLIQLTPNIFNLLQERLQIAQQIDCHDAALILKKSSISLLEAIILKQYDSITVQQYEKIIVFLIQELIKLSGGDQVDQMYYTIQYKNNILEILNRFILKDLQSMIQVLYSAQVQLDQYFTLWQETAQHIINRGNRKINLISQLLFLKYISKSTFEKLFQHVLQDAFPEIDYDFELRSQEFREFQQKNKKKLANSKQIRLSKQFRNNFRKEILQENCLYDETFNFRSYFFQSIQECMKIHNYSELTQIITIPSIIDKLNQLLNEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TJE3</accession>
    <name>A0A8S1TJE3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0220112</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8151804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TJE3"/>
    <dbReference type="OrthoDB" id="415459at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051252">
        <property type="term" value="P:regulation of RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000003">
        <property type="entry name" value="Zinc finger CCCH domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000001">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF18">
        <property type="entry name" value="PROTEIN TIS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25427">
        <property type="entry name" value="zf-CCCH_UNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="78"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="78"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8151804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="222" mass="25739" checksum="AE9F1B2334FAD23E" modified="2022-10-12" version="1">MQVNQVQFPNQKYKTQLCRHFITNGNCALGARCQFAHGRQELRANANGFQQNPEFIMHQNPQGALPIKTQPINPMIANYKTQLCKHFNPQTGLCKNGATCTFAHGEQELNQINPYFQNQYLAMQQQIKQQNQAQIQAELTQQILIMILSNMEHIFPGQQDIINLLKQGQEKAKQGDNQGASEIIKLIIHDEQRTKEEKQQYQQIYNNAQRHYDSKLKEYLNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TJE8</accession>
    <name>A0A8S1TJE8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0210265</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TJE8"/>
    <dbReference type="OrthoDB" id="289628at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8151262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="292" mass="34648" checksum="17A087A7509FEE16" modified="2022-10-12" version="1">MRIFKQAFESIQGKKGEEENKSGQKGAFYIDEQFMTPEFLYKQNQKEVQQGTQKKINLTKQFFLTMSTMENKFNLSPIKQINMVDQLVGMYQECVQQYDTLMDPIKYYFLDKIKNIVQQADKFTQREPRSLQSSERKIQQPIQIMSSDVSYFKDFEDFNDRIDKPTEYATPKNEDQFQQIDNKKLVIQLQTFIEEGKKNKDNPDIIMKNHESPQTQFNDTKVIQKLDESSSKKRSPKIEFRNENNETPIPKLLLKEEHQVKQNVVESQLQNQSKSIQDRLMARQKSQKKKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TJW7</accession>
    <name>A0A8S1TJW7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0210360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TJW7"/>
    <dbReference type="OrthoDB" id="510307at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004452">
        <property type="term" value="F:isopentenyl-diphosphate delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009240">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04697">
        <property type="entry name" value="NUDIX_Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10885">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10885:SF0">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="1">
        <location>
            <begin position="36"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8151452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="178" mass="21156" checksum="A4BDC865C76B03DC" modified="2022-10-12" version="1">MKDNQNESELLKAKVVDINNHVIDLICIDYKLLSKVIHQATFLFVIEKQTFRIFCHKRSQQKQYQPGTLSTCFTGIVLESEDYRQCAKRKLIQETGLDLELTYLNSFYFESKKNKIWGQIFYTFYDGQIINFIQDPKEVEEIELMSYKEIVIREQNGEIFTPESIQALHFLNKRVLRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TJY4</accession>
    <name>A0A8S1TJY4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPENT_87.1.T0220237</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8152017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TJY4"/>
    <dbReference type="OrthoDB" id="435193at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000315">
        <property type="entry name" value="Calcium-dependent protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="46"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8152017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="200" mass="23546" checksum="E50D58F1BCCC4725" modified="2022-10-12" version="1">MQPYKKVCVQETNIPYSFSEQDDKIGIDYSNFIQQMEGPYNQLYEPIDKQCIGAGGQGCVIKVRCKLNNEVRAMKVIKKMSEDKNENFRKEFQNLKLLDHPNILKLYHSFEDDQRFYIISELCEGGTLSQYIEDLYPLKEAEVLKIMKTHLIEQTKQFEIHAVSPTHKKDQEDIKSDSLLFSSSEKFKKKQGLYPDYYYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U168</accession>
    <name>A0A8S1U168_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">poly(ADP-ribose) glycohydrolase</fullName>
            <ecNumber evidence="2">3.2.1.143</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0310181</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the poly(ADP-ribose) glycohydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.143" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U168"/>
    <dbReference type="OrthoDB" id="1937899at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004649">
        <property type="term" value="F:poly(ADP-ribose) glycohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990966">
        <property type="term" value="P:ATP generation from poly-ADP-D-ribose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009225">
        <property type="term" value="P:nucleotide-sugar metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006282">
        <property type="term" value="P:regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046372">
        <property type="entry name" value="PARG_cat_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048362">
        <property type="entry name" value="PARG_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007724">
        <property type="entry name" value="Poly_GlycHdrlase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12837">
        <property type="entry name" value="POLY ADP-RIBOSE GLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12837:SF0">
        <property type="entry name" value="POLY(ADP-RIBOSE) GLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05028">
        <property type="entry name" value="PARG_cat_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20811">
        <property type="entry name" value="PARG_cat_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PARG helical" evidence="6">
        <location>
            <begin position="5"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="PARG catalytic Macro" evidence="5">
        <location>
            <begin position="118"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR607724-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF20811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8158124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="347" mass="39871" checksum="524DEE86E7E5F330" modified="2022-10-12" version="1">MKCNEKFKIKTLPYMIESVQLLLQSKPQIQLLLSNTTLTLSRQNVFSLLSLMYFELTIQQSVQNFKFPQYFNFSTYYMQGSQLEKLKCLINYFNQCVEYPEYFNLPDIVYKRVQLIDIPNWINSQIPLSGFKFEKQKNEDHLNCGIVDFSDRFFGGKVAAGRGCAQEEVLLLINPEAIIATLFTSQLGDKESLIIQGILQFNEYRGYEDSFTCIPIKYINKGQTLIAIDALYFATKPQGYQFTQEAILRELNKSYSGFKGSQQQVISTGKWGCGIYGGDKQLKTLIQWISFSQACPDGNIIFNGLDDKAYHDLGKRLELCKKRYQTVGNIVKEILNSSQKNILDKLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U226</accession>
    <name>A0A8S1U226_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0320036</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8158578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U226"/>
    <dbReference type="OrthoDB" id="20524at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="24"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8158578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="571" mass="67200" checksum="43BAFFB0A4A74E0B" modified="2022-10-12" version="1">MKFAQVTAGAFQSSKIVKVEQYSYSVNDQIGSGFSSQVFKGKNDNTNETVAIKIIDRSKITNEVEEFLLNQEIRALSLMNSENVLKMYDYYHRPQCTYIITEYCNQGDLGQLIKRKQRIDEIEAIKIMKHIVNGFKEQVAKGIIHRDLKPINILIRNGIPKIADYGFSKMMNAPPETIYYNVGTALYMSPQTMIKNIYSEKTDIWSLGIIFYELLYGQVPYSAQNEKDLAQVILKTQPQFPANIPVSKETIEFILKCLSVDESKRYSCIDLEHHPIFYRRHTMTAPRPIADKNTSVKNRDNYSPQRTTEYHHNRKINFIAQTAMPQSALKSRESVRYLTQNDEIIQSQFQFIELMFRILRILDKQQIFNQDFQIKLKFLIIKNMFFKTILLREVVEKKKNVLQLCEFDTYIETVGKYQTQVHEIYQIAKEYHDKQYQMIESNHTLKQCVLRDRSFQKIYENFKSVNESYEFYLLFSTLLQRCIRDLFNKCNSRLAGASDSQQLQLQDETMVYVLEQLTYYYSLLRLIIENNNNIQIFTKKAQICRILQATPEQITKSNLMSIRQSIQEMKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U252</accession>
    <name>A0A8S1U252_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0320013</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8158534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U252"/>
    <dbReference type="OrthoDB" id="295410at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="127"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8158534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="456" mass="52854" checksum="AE1161BC44856C8D" modified="2022-10-12" version="1">MLIGNDQNFQCNQLLQKDSIFNDGIALWIHQDYDSSQPIDQQKFKIESDSKNKIKQLTLYEKYIRYSQHKIILFENSFLDLIHNSQNKLVGFKLVKYDGSLTIIGECKLWIKKLKKYVIQSDFSRNYKISKKIGNGTFADVYLVKHKQNNKEYAAKIFYKKKIILEDQQILMLNKEVQITRLFSNPNICQLYEVFECKVHLTLIMDLLAGSELVYSLKLGGLSEIQSILIIKPLFQALSLLHKQGIFHRDIKPQNIMLHQSGKLETACIIDFGLADFWNDKNEYLFSQCGTPGYVAPEILLGHSYDFKVDVYSLGLVLYLILTGKEPFQSKTMDGLIEENTYGHIALSHLNLSPNCLDFLSKVLAKSPNQRLTSQEALFHPFLLLGPGNHLDLIHNKIQSHEQFSSKSLQFRKSVASVRLQTDISNNQFYQRDIFKKNELERKKIQKQFSIRKVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U667</accession>
    <name>A0A8S1U667_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0310182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U667"/>
    <dbReference type="OrthoDB" id="302223at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8158126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1094" mass="130107" checksum="D487995379517B09" modified="2022-10-12" version="1">MIRLKNIIGSAIHIQTSLFRFSTQTPQPPPPPSDQSQKKVLFSGKSADKPKKQHQKSEQKHQQQQKKESNENQQHEQSPPQKKQERHFDRQQQFHKQKVDEPRSYKQDRDRRSPDQQSQQQESPKRTGILFGNKSRDYKQIGAKYLSKRSFNQILKKKQETEKLNEKIFIDQLNEAQNIQNIHERNSRLIDVYASQSKWDKVKDIFKSEKQFNSGVYSAYINQIASNYVDLSEYEQLKVIINKVGDKVHTLKPEVFQSIYFMIFNLDLFPNEKAKELEYLIDIVTTHHLTPQFDLTYFQTLFEEQSLESEKTSHLMVIKVFNQYFERHAQQNKGKSTIDLPNLNIAFSVAVKSTIFYNTNDAQNFLNNLKAFQQLAENNVKLDDLVRYAQYISLKENSFTQLIDLFKGIRNPNFIFLFEEALKKLDTNGMEVKAIDKQQLEKLYFEKSENLPVRQAELFGLFADRFQYPELTVEIFQNHKQNKQNEYSSFLYQKAIGVLIDQSGGKHLSNSVMTLLQEANSFKVPLSKVNYQINQIKAHIKEGQYQLAYNLFTQNVIDDIILAFQKEKIRRFLYKYVQKIHIGTKFRLRSNMKTYQKKIKHIDEFVKYLSDKQLFDFYKEDRSDNFLLESLIQQEDIFEFNKQITYREAFNQIKTEVAHGTFDYKKLIQPVTESEQILREVFDNYEYHVKIEKKLLFDERKRKRIQEVINQLRQRAGQRKTYEQTLAEIEGLFKKSTIENGLMRNDLIEFPEVEQIQQMQLKMKKELKQLGLPVEKYKANVITFQPLQINSQKEQEVIEQIPNEVLKKDGDNSKQKGKSQKSAFKSVDKLIDEYIAKARDDQKNLRNIEKWRRYLKIMYSGIQFKHKMQTDKNFFKKMIRQKYQYGSQDIGMKELINLKLKKKMLNRKRRWFTRNRLREKPPKISVFINGRVSLSDFQFMKYIYYNAYTQYDEYINVPPSNYQNEIWDLLAWGIQNKEPMAVKLAELYSHTCGAKMPEYLTKLIANFYKCEIGETNSEIRQRWIQSLEIVNDISTNKVYSHTNDQAFVSQQDIEFLHALECKQEYKGICRALLNTTHSYRKYVRYGVSQKELLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U8C2</accession>
    <name>A0A8S1U8C2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0330294</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U8C2"/>
    <dbReference type="OrthoDB" id="10383270at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8159729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="344" mass="40782" checksum="62AF6C78D4D225BC" modified="2022-10-12" version="1">MNENKIIQKSIINYTEFTDINLFANKQVSAHKHPINTPQIKNYSVRVNDSLISPNQYLIAIRLQINPILKEKEGFPIVQLYNVITGKELYSKSWKVDSSLISFQFSFDSSLFVILDTESLSIINVQEINKIQIIKLKSERLISIDTDNNISLQLSNNSIMVYSPKNEFQHKLQIYNEVDYLHMLDLNYAMYSCGLYQYLLNVQNKRVLRRWMNHDFEITNQINYKNFLVIQSCGYKMKFVYIRLQQSGKIIRKYTSCNMRQGQNRIANENSSILIFQFVYCRIKQPILNDKKSVFKIEMNVNKFDLLQGGFKQYNFEIDQDYDLRKEIPIYFLNGNQLNYYLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UDX2</accession>
    <name>A0A8S1UDX2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0370099</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8161929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UDX2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8161929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="111" mass="13283" checksum="1A4A511AD2E82FFE" modified="2022-10-12" version="1">MNQFCQVEQNINKDILKQFEGQLRNYCKINVKNNQDHQTQLVSQELEIYIPSSNYKLLIKSEDVEIQNNLIDLSQAREFKQNIHKLNHKFCLNAYINRRYISNLIQKLSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UE37</accession>
    <name>A0A8S1UE37_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0370122</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8161952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UE37"/>
    <dbReference type="OrthoDB" id="283014at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8161952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="18842" checksum="ED873A335BB77E63" modified="2022-10-12" version="1">MDLQLQEQLNIKRTLEYWIPGATLEQMKQKLNTKPISQFKKEEFLNEIKFGKANIYQQDDKYLSETEKAYLIAYNPEKRVRSDYECMFMSNDEKTSDREEQTNQKCENPNCPGTDTKWIECSEKKCWYHQSCAGIPLNKTQEEIDQMDWKCKKCRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UIJ1</accession>
    <name>A0A8S1UIJ1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0390283</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UIJ1"/>
    <dbReference type="OrthoDB" id="295175at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46726">
        <property type="entry name" value="TWO PORE CHANNEL 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46726:SF1">
        <property type="entry name" value="TWO-PORE CALCIUM CHANNEL 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="213"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="279"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="428"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="462"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="493"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="564"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="627"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="667"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="359"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8163562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1187" mass="140595" checksum="8365D842225332F7" modified="2022-10-12" version="1">MKKTHKKQSTIYRQSIAMTEITQKLIEKNDVLAQEIQKIVKKLSPESRIEKAERHIYDAYYLRAIRNDIDLVNKKYLKLLWYLENNQYWLSFLFILSIIYQLITFFESSFPDQDNMENPNLFIVELIILLFMGIDSSITIILLLTKKENRFNFNPKKQFKMIVYYFCLVDFIMHTYDPTLFRVSKICRTMLMPMYSKDLRRNLKGIMKAGKDLLLLIMLYIIIISLFSFIGINFIGKIDDVDLKTQDYGDFFKFFSMLFMVATLDFYPDILIPPMLQGIYYCLFFIVYLLLFIFLFAPIPLAVVYEGFRRHRMEIAISDIIKQKTAMMASFISLDFHDQGYLTRDQFRIFIHHFYKNTINESQIDQLFNQIDQDFNDKVQFDEFHKFLHLLQEASILSLPEMKPLHCWESFRNYLISKGLLKFVEGNIFGISMLIITISNCILIVVAFFVEDQDILDIFNLLDTLFLIIYSIECLMKIIALGIKSYFEEGWNIFDITLVILQIIFDYILFNIVSGNIVQSIKANRLLRLAKIQKVFRLFRAFRSLKILNILFEGLQFLDVVRTLLYKIIICVPLILRLMLPVQMIFFIYSCIGMYLYGTIQSNHNNPYANSECNINNFEYKWGNCKYADFTTFAGSYLMMLQMFIAAEWNQIVFELTYDTDDMLSAMLFVGSFEFFSIFLLALIGGLVWEVFTVVSQSLKQDEDQLNPIQNNLDQKQEVIYNQAAKHDKKKKLILNDDNPDEIKFHRKFRIDNISKTHTKLYNVQQVFEDDIINDERPELNISQPGSRTYFLSQGVSGRRVMTFQNSKINVQRNDIVNHFSKNFISFDIWLSTKQEEGLDLNQVAIDYMIHLRSEIKKDEMLQKKNLNISNHHLLIQNAVLRDASEVYIKQKEDKFFKTSFKQKFERIQELKFQNKFKIESKIIFSLMGILKFPKPNIKYYFQILNLIENYFTYQLLSDYSFFKLIHQINNKWYTISIEDGQIYFLKIGNGPWDYTEQLFQESNMRICEDLTSLFETNNQECIKANEQFSISVESIAKNFEINISSIEMNNSCVMYQIKNEKLINNDIQSFKFKKSNIFSDQSPNNKQYVYLDSQNQINGTLKLSQSPKINMSNLDEQKNVSQTILFVIARTTAQEQKIKYQNITMLTQFLQDLGIVIQNYGSNFFNQINNLYELRYQHKIQSMRKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UK40</accession>
    <name>A0A8S1UK40_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Golgi apparatus membrane protein TVP23 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0410232</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TVP23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UK40"/>
    <dbReference type="OrthoDB" id="2151161at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008564">
        <property type="entry name" value="TVP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13019">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13019:SF7">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05832">
        <property type="entry name" value="DUF846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8164825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="238" mass="27425" checksum="163043F69E9F6871" modified="2022-10-12" version="1">MVQSNMVNSNLQQPTASKINEYKQYKEKNKQDMTTSVLSLNGFYKEQMEGYNFDYTKSDHPTVALFHFAIKGFALVVYLFGGLFIREAWTLPEIVIVIGAVDFWVTKNITGRLLVGLRWWEEIDEASGEQLWVFETRVNENQVSQVNSFIFWATLVLTILAWGFLAVVNVIGFHILNLTGISFQLTIQIINFYFYFQCSRVAKTRAKQLAKVLGAEILNKMMNQSVIWPMRVPKQKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UNP5</accession>
    <name>A0A8S1UNP5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphodiesterase</fullName>
            <ecNumber evidence="1">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0420175</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="1">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UNP5"/>
    <dbReference type="OrthoDB" id="189220at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="2">
        <location>
            <begin position="717"/>
            <end position="1054"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8165362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1061" mass="124332" checksum="CF1E57503915D2F8" modified="2022-10-12" version="1">MDPQLSHYTQFFIVVTNLCKTNQITPEQKSLLKSNLTRKEEKICRVLIQNSGPGKEMLLREALLDYLAAQNKQIQRRRSHKSKTVHFMKDVAEQEKQPEVVHQQESSAQPLASAAAVMIEQLTGVLHKHIDKHSLLVPEDKEKLIQLCSLISKLTTEQVDLDGVSPFRARYSSENSKLAEISEKDLDSSDEDVYEKMSKGIDDIYKELKSIFTGNLHTHLLLMQEDISYENLYQVLKFLLKILVDADEFTFFIHRDKEWEVYQSSNDSIKDVTPEEAQRVTDELAYIRPFCIHRIDPKQRQYPQIEDTCKTIGYAQTSIVKFQLQLKNYEVLFCLHWADKDKKNIKRKFINYANMYGFNKDVTHLAQFLVETIITAKVQFFNPLRFADQVQDIGISFMRISRFLLVEGIKKVLSIKFEVDKEQTFSTDENIKKFKESQCVKIELKDSNPVTLRIKDMDLKNEQDQHLYQVIIQIQEKYDGYVKLCYEKSAFYKYFLRTTDSLLFDFNKQGELIFLSRPISKSLKERYSINFDPKAILYNKISYQDIFAQNSIISNIEVNIQNIHENRRNQYLSNLENPQFEIFLKVVDNDFKGFTVIFHENEARRLKQYFMALKIDNMTNDVQKEAEANKSLEEDIQKQILIQYNKHQTFKFLNQLDETQDVANSMIALFIPENELQQIRHRKTDGRSPEQQSKDLQNDQWIIPPQKKKKIGSSVYVKYQQQLETVDANLFKVNDSDSQLDLFEFNILILDSSQEKHRIVYSILEKNGFISQYNLNKQCLAQFLSVLQKKYNKRNNSFHNYDHGIAVMQSAHYMLQCGKAKQYIDDFRRMSTIISGLCHDVSHTGRTNIFMINSQSKLATRYHDSSPLEQHHAATTIFMLKDPSLNFLNTLSKEQSQQFRRVLIDNILYTDIKVHFTLLKDFESRIKDEVTKPFGTGDDDLKLLTGMIIHTADFNGGAKVFEISRIWSERVNKEFSAQYDEEGRLGIPQTPFLKDLDKLHIMAKSEMGFFKVIVRPLWFTLNTFFDGYLQQSITNLDNTIISWEKIYHANLPKEEKQQQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1URX7</accession>
    <name>A0A8S1URX7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0460141</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8166927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1URX7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8166927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="56" mass="6865" checksum="254F70ED918DD18F" modified="2022-10-12" version="1">MMKIQKIYIKECKNWIDYNIENSKQMIYKGCLILSHEVEYVNKKSRKHFLNICKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UUK3</accession>
    <name>A0A8S1UUK3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0490043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UUK3"/>
    <dbReference type="OrthoDB" id="302832at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8168351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="494" mass="57753" checksum="4344993240478B26" modified="2022-10-12" version="1">MNYKPISDQQTLDIVEDDGELDVVNIKQDKRLNKTNNKLIFLWIIILIILGGILIYVSQQSKQQKKIISDDQSKINNTITIVQYNKNNESEFDSPKLIDLDDQNKKEEDGDLKKDTELQNELLPKEQDIPQLNLNKSELNQTIANQSQIQNSITNGTEHLQQKDTHQELQHSRNDEILCEKGYYMDKNIKGCKQCEEKCSECMHITGTCYLCEDEYYLNIYGKCINNCEQNQKQPNLLISEDNKNQTIPFCYGKKHNSSFYMGVQKSKSTHFVLPYLIIHPAISVSQVYHNNFPIVYYFNKDLFSFQDNIKLTDITQEAQERLLIVVIVGCHTLYDYTLFNTFLFQDAIENIEQIVKAKSTTFRSYFGQDYNGYGVIKEYISQYRNNTIDLIISDDPSEILHEEVELNQLVTSERHMTDYNFESLSGAKSEKSVVVLLQYTIKDENHNNNNYCDRFKYVSFVNCQLREVQISHIQKMKQNFEFISQFVVQLNLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UZC2</accession>
    <name>A0A8S1UZC2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">RING-CH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0530089</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UZC2"/>
    <dbReference type="OrthoDB" id="264354at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16495">
        <property type="entry name" value="RING_CH-C4HC3_MARCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46065">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCH 2/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46065:SF3">
        <property type="entry name" value="FI20425P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-CH-type" evidence="12">
        <location>
            <begin position="808"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="816"/>
            <end position="866"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8170498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="970" mass="115830" checksum="157837BB400F9DB7" modified="2022-10-12" version="1">MSKQKQTDNKTNEQIISDLRFLFEVTSNKHKQDKDFLKKFTSFIKKEKLTDKKNIVNEMFNLFKSASNSKALYFNTIIDCLLLIQGKDININLVLAEVIKGPCQIDKKSKENQDNEEIKLFCFQKLEIQHIENQLNLSKVLVFMERVLKVISTKTKDEIKKMFQFMTSIQDILSQQLQLQSLICILILKIVLTLKKESALQKTDMKVLYYLIDEQSIHLVFKNILTIAKTTYVHKYYHYIFKSCFMLLCEICIKNKMAVYFQTFLCSPMSQTHLKTIFNIIKEFNYQFFQDIKDLPYLFEKPRFLIDYLPTNFLLGLLNRNPGNWMCVQNFIQNHIQIDDKYLNFTLANLAQRQSKIVDPGYIFQSNKLKKKNIIQIDQLLIESIQQQQDVGDYNTYIYCQLHLLLYLNSYDLTSNYFNKTQISCDSRFFKRLFLLKLEDKIYKYQEITRLSKPRQGINQYFTFQINKQLKQLKYPIAQNQIKNIYQDIKQMLNINYHFQFPFISKFSSYIKVLFKDLTQQFLNNTLFPFDYQQYFGLILILSSYSKQAFQAFIFSIFNSLLAMKKPHNVHQELTPLQLSALEEVGVMFNEMNIEDSIFQQMIITSIHFFTNLEIKKNSKLNQFTQILERLKIYLRLNWKSEQSVQKQYKQLMLMAVDRFLMLNGLGTQSLKNNQSKFLRFFNSNLVNNIDFIEVLIKLTQHIKIQEVSKYLEYLGECYLGSLAIHQQNRSLKFQLCTQILYHHRKNALIYLEQNMKDPNQLYELLYFFQREKRLKQEQPNKEISLFVYERIIIYKGALQLFIEIIKMKKKQKHICWICYQTNSHKRFKPCYCKGSLSYIHRKCLNEWATKQYNQNNQIIKCPNCKYEYLFAIKECYKLRNFSTWQFCDSIEKIVLICMTLSLIIISGLDFMSVSDYLQVDENDKQEVTMFKWVNRVHFGTVLIILCSATFNIVQMAVSKTELEILDIRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V1S0</accession>
    <name>A0A8S1V1S0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein-L-isoaspartate O-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0540002</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8170881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V1S0"/>
    <dbReference type="OrthoDB" id="73890at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004719">
        <property type="term" value="F:protein-L-isoaspartate (D-aspartate) O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000682">
        <property type="entry name" value="PCMT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579:SF0">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE(D-ASPARTATE) O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01135">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8170881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="16178" checksum="2DCEBCDB51B82366" modified="2022-10-12" version="1">MHAYSLELLKDHLQNGVRALDIGSGSGYLLEHVPELVEKYIKNLSLSYNKQLKDKQIQIIKEDGRLGFEQEGPYQAIHVGAAAEIIPQKLLEQLDKGGRMVIPVGKGNQVFQVIDKDQNGKINIQNVLGVRYVPLTDLNKQLYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V368</accession>
    <name>A0A8S1V368_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0520193</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000052">
        <property type="protein sequence ID" value="CAD8170179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V368"/>
    <dbReference type="OrthoDB" id="5800476at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001190">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="8"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8170179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="294" mass="34548" checksum="0A0B2EF0A7AE3570" modified="2022-10-12" version="1">MQKIGQNFELKKKLGSGAFGDIYLGVNKKNNIEVAIKLEPVSSQCPQLRYEHEIYKRLLADNQSIDRGIPHVYYTSEEGGYTFMVMDLLGASLEEIFVSKKKKFSLKTVVMIGLQFLERIEFVHSKGIIHRDIKPDNFLIGKLNKQNKIYILDFGLAKRFIKENGHIQYRESKTLTGTARYCSLNTHLGVEQSRRDDIECMAYVLFYFLKSTLPWQNMRAKNKREKYERIMEKKMTTTYDELCKGVPKEFGQILKHARNLEFEEEPNYKYLRGLLESICQQEKITVDYQFDWCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V8L2</accession>
    <name>A0A8S1V8L2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0600105</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000060">
        <property type="protein sequence ID" value="CAD8173878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V8L2"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00882">
        <property type="entry name" value="Ras_like_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8173878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="419" mass="48797" checksum="A5AE1402B514E5EF" modified="2022-10-12" version="1">MQNQHIRTYLVYGDSGSGKSTFINSLGGIDQDNQKPQTGGLGVAVTIDPGRIYYIQDEVLGNMQLIDTRGTWDPGQINNDQILQNLVKTLLDYSVHEMSQQVNPIQQLDGILYIHNSFIDRYARDTRIKQLGILIGSNLHTSTLTIMTRCKEIQNTNLKFNFYEWNKKLHNSEIEYIHWDSVNPLENQKEKLKELLLNLKPIPLSNIKDANQEIIKLAKELQLKDLIEIEEPRVRTIIKQYTKEKLKKKTSYTNVTLTINPPKGIMNQIGGWVPIKIPYLSGGGNLTTTSIRIDADFPSYSLPPKLILYCDLDKSLNEIKGQNLEIIKHEFINNGDYYDANFQYRWNGINNENVGRVDLKIKMIIQYRQIKQKQREDKEEYFEKVMKPRYSDEKPYIQQAMEQQFDNLYEGIKRNFEKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V8X4</accession>
    <name>A0A8S1V8X4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0540253</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8171356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V8X4"/>
    <dbReference type="OrthoDB" id="294912at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="377"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="451"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="385"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="451"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8171356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="502" mass="59443" checksum="D06248358370E0F9" modified="2022-10-12" version="1">MHIEHKSNKIINEFVKQFPEEQRNSVMNFLLILGFDIARKLGQNNQAELYSTMKQLSKFIINDYNQNGQSELREEIKKIQQQLVQLNSLFNNNENEQMQSKSQDIYRNQPQTKPQYSHNRFYTQNDMEDQINARMREEQQLSTNSKNQFKPAKRNNKNKSPLQQNNNNLTAKITESPPPPMVYQKTVSSKFNIKPHLDDHQIKQLLYSENERIYQEDFDSISNRGHHYNQSPKSIKYTLNIQNLDNIGQRFQTHETNKKNKPQQTSNSSRQKKKVPAYLQNVESKIKPKLDQDKQAFKNQKNYDDDENQYYNYKMSSSQQMTANFQNNKNFIRPSQESDTYYEIQQYLINQQHQNFHLSPVQEMKTDNEIISNKKKTQNFRENTKSSIKSSNDANNLRQHQQQHFFEQHESLPYTNSITPIQVSDFQTSKQQSDLSFDAFRKKAQFTSPYAISLQQSSQPIQTAQKPQRNDDESSESFSNFTPPNKEVREFLSKQCIYQDQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VBM3</accession>
    <name>A0A8S1VBM3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">USP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0590121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000059">
        <property type="protein sequence ID" value="CAD8173429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VBM3"/>
    <dbReference type="OrthoDB" id="286820at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031647">
        <property type="term" value="P:regulation of protein stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02257">
        <property type="entry name" value="Peptidase_C19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000267">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF644">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP" evidence="1">
        <location>
            <begin position="445"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8173429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="764" mass="90499" checksum="844779DA5F605393" modified="2022-10-12" version="1">MTTILDNVTTLKDKIKILDDNKNQTKNLDLQAIYKHIHNIGQDSNQLLEHALKINFGNVLKIQAQNDQNEVLQVFKKVCQTAKDLNHICIYVKYIQQISKQDLQKIYFKELGFQTEKLITEFRKQGTIIKELRLDTNDITKQRFMIQMITTLCPQDLGFNPELFSLMLIPFKCLHKADPTDISLLEKLADVLLSQCNGQGIANQNQVHDWLALLTGIIQYAYEGNSIGLFILLRKIPHQHINELLYRHKYFMLLNYFNNLKVTLKIFQGLTFYSITLQSIFFIRTISQFLYEYALEIKSKQYSDILKYIYQEFILTTILGNIQMIHYSTELIDLLLFYIIGMSSIDQDPLLKSLDLLYFKLKQASNYKIFTKDFKTQRILFLLTVAMNYNGLNTDEQFLNIIQIYQTTDKMIPYKQYIQWDFSQLIEQQSQIDQNILLRDSKDFVGLENLTNTCFMNSILQSLYMTQSFRKFVIQLKFTDQSLQLIPYPSKLSSLQKLFLLLTYQKQGYCSPYELKRQLREPYSNTNDQQDVSEFAHHFLEDLLELLPKHLQIPMEKIFFGYHRSCIECYNCPKRQPAYRPKEKFLGIDLHFNEDEENQDLYDMIRKAYEKEQIEFTCDKCNQRTDRVYKSQQLIQLPSVLFMIVHRFTFNAATQTMNKMLTKVPFKFQIDFRDVFNQKKLNSKDCIYDLYAFIVHMGKNSYSGHYICYARQLNKPDVWVTFDDTMISNLEYDSDQLDKELIAEETPYLLFYQNQSGQPLIFNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VDE9</accession>
    <name>A0A8S1VDE9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein disulfide-isomerase</fullName>
            <ecNumber evidence="3">5.3.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0620026</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Catalyzes the rearrangement of -S-S- bonds in proteins.</text>
            <dbReference type="EC" id="5.3.4.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.4.1" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8174847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VDE9"/>
    <dbReference type="OrthoDB" id="2121326at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005788">
        <property type="entry name" value="PDI_thioredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057305">
        <property type="entry name" value="Thioredox_PDIA6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01126">
        <property type="entry name" value="pdi_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45815">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45815:SF3">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24541">
        <property type="entry name" value="Thioredox_PDIA6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035850590" description="protein disulfide-isomerase" evidence="10">
        <location>
            <begin position="16"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="11">
        <location>
            <begin position="122"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8174847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="386" mass="43489" checksum="88689E40B7F44532" modified="2022-10-12" version="1">MKAIFFALLIALSLANFSGTDVQELTQDDFNAKVQNQKTFWVIVEYSQLTQEQRDQVVSAAEALKGMINVGALSNGASTILRVYSNGQAIEYPGEWEAQEIVNFAFDQVRDFAFKRVGKVPKKQGEKTPEPQIDESDVIVLTDDNLDETILNSKDAWFVEFYAPWCGHCKKLAPEWAKLATSLKGEIKVAKIDASVEGSKSKTKYKVEGFPTIRFFGAGEKVDGDYESFDGARDYNTLLSYARETNRKLKPLFFEQLVNQQQFTDNCQKSTGICVLLFVPHIYDCDQECRDAYLNTYKETVKPLKSKPLVHFWSQAGDQYELEEQFGMSGAGYPSVLALSPKKQLFSKMRGSLTSANVDRFLNNLLNGKEQVSRFGSIVPIEEVKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VDK6</accession>
    <name>A0A8S1VDK6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0610222</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VDK6"/>
    <dbReference type="OrthoDB" id="297145at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="24"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8174671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="215" mass="25527" checksum="FEF78B9124D06ACD" modified="2022-10-12" version="1">MQLEQRVSNLEKLTEQLLGRICELEDQQGDLQDQIKKLQTKNQQLEQEIGNLKNKTEEIQESWLFYCDKKRSLNSIKQTLQIESDIVKEFDYQSWLTEDIMWRQIIKNICKELQKDLEKLNGAQLKQLAVQKLKENIDNEVLFVLRNVNKENEKMNELIELCAIFTQLWYEIELGGEQCQGRMILVIESEINLDKLELTRQDNSKVILQIEKLQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VDV7</accession>
    <name>A0A8S1VDV7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0610208</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VDV7"/>
    <dbReference type="OrthoDB" id="301022at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="421"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8174643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="531" mass="62581" checksum="4BDBD8F3042B5BE3" modified="2022-10-12" version="1">MNWDFLNRKLKEFFEQRRQQSIDKLFTLNTVTDNNFLKKQFLELETQVTEDPKQIVSQPIVSITSPTPQKSKLTPKSFNQISHLFSPHGPARSRNSMQMRQIVTSNLKNENNSYNNVKSKDIKKVQELVGSNNKTTYKSSGRKLQKQQTSVSQKILKDLDDQFDPSPSNLKKPSETLKCFITQTKNINTLSMKTQLQLKSQRSIRQLKQPSPALDNFTKMGFGKSTDPRVGLDRFLSNKWLQFIIEQKSMRKDDQFDNYVKQYNESPQKEQNIQKLKEKQQIRQEFNSEIPQTIIFKPHRYTKKISKINKSQISELISSYSQTVFDIDKKRDDEELIEFYPIYYEKKVKKRRIIRKMKILAKRAIKQRTFVLDLLQNFCQLVPYQMPGSIAFLRFVQYNNIQAVKQMLDQDKSYIGSFNEQGQFAIHIGVMIQSIEMVKLLLYYGANVNSEDFFLQKPLYFALVTDNLQIIRLLLACRASPWDINLRDYFICCRSEDAKNLIKLAQYLKIYNQDPECFLNIENIKQQLNQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VFH5</accession>
    <name>A0A8S1VFH5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">cathepsin L</fullName>
            <ecNumber evidence="10">3.4.22.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPENT_87.1.T0630002</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="11">May be involved in extracellular digestion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Specificity close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity.</text>
            <dbReference type="EC" id="3.4.22.15"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.15" evidence="10"/>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VFH5"/>
    <dbReference type="OrthoDB" id="190265at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000104">
        <property type="entry name" value="Cathepsin L 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035812952" description="cathepsin L" evidence="12">
        <location>
            <begin position="24"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="14">
        <location>
            <begin position="36"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="13">
        <location>
            <begin position="119"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8175331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="324" mass="36735" checksum="B2BEA9AC4D0CFA28" modified="2022-10-12" version="1">MEKIFLTVGFMILLGASLYLNNTQEVSDEIDTGNLYTNWKMKYNKRYTNQRDEMYRYKVFTDNLNFIRAFQESRKETTYTMELNQFADMSQQEFAQTYLSLRVRKTSKINAANDNFQYKGAEVDWTTDKKIKYPAVKNQGSCGSSWAFSAVGTLEINSDIELNKRYVLSEQDLVDCTRPYGNQGCKGGWVDLAYHYVLDYGLTEAKDYPYTGKDEECQSSANRPFQHLAGYYLYETCSQLADAIQKRTVAIAVDANSWQFYGSGVLSECTKDLNHAAVLVGVSADGVWKIRNSWGATWGEAGHIRLTEGDTCGICADPSSPVLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VIS7</accession>
    <name>A0A8S1VIS7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Core Histone H2A/H2B/H3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0680088</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000068">
        <property type="protein sequence ID" value="CAD8177798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VIS7"/>
    <dbReference type="OrthoDB" id="306018at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22911">
        <property type="entry name" value="HFD_H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45810:SF1">
        <property type="entry name" value="HISTONE H3-LIKE CENTROMERIC PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45810">
        <property type="entry name" value="HISTONE H3.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="5">
        <location>
            <begin position="79"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="34"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8177798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="185" mass="21734" checksum="987AEBC61BD1AB23" modified="2022-10-12" version="1">MANRKKKAKENNNQSFQVDNNEQMPSFMHSLFNSDEKSIIEEKSNEESKKSERKKDERISGIQVQKARDKLQKRNKPMSKVLQEIRQLQTSSILVCRRAGFQRFVRWAGIKVSDELGFKEFRYSSKSLECLQTLTEQYMVDLFEDSVQCTFHAKRVTLMAKDLNLTARIRGIEQPLQEIRNLKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VLC2</accession>
    <name>A0A8S1VLC2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein RER1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0670144</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the retrieval of endoplasmic reticulum membrane proteins from the early Golgi compartment.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the RER1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000067">
        <property type="protein sequence ID" value="CAD8177343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VLC2"/>
    <dbReference type="OrthoDB" id="448250at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006621">
        <property type="term" value="P:protein retention in ER lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004932">
        <property type="entry name" value="Rer1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10743">
        <property type="entry name" value="PROTEIN RER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10743:SF0">
        <property type="entry name" value="PROTEIN RER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03248">
        <property type="entry name" value="Rer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016013">
        <property type="entry name" value="AtER_Rer1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR016013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8177343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="172" mass="21009" checksum="3EB3BAD3FB54331C" modified="2022-10-12" version="1">MNSEIDPVYTRLYHKYSMKYRRIIDKWIMHPGKRWALCALLLFFYFSRLVQTESYFVVSYMLGIQIIQSLLRYFTPLGLPDIEDEDEDVNIQLPQHNDDRPLIRSMPEIQLWEQIVFALFLSNFATYFQIFDLPVYWPFLFSYFILVMIITFKKYLKHMQKYGYSWGDFSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VMN5</accession>
    <name>A0A8S1VMN5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0700044</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000070">
        <property type="protein sequence ID" value="CAD8178664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VMN5"/>
    <dbReference type="OrthoDB" id="295823at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="668"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="367"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="684"/>
            <end position="693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8178664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="693" mass="83238" checksum="6AFA57D27B1AA754" modified="2022-10-12" version="1">MQNKQLVTRTTTRRNLLQSQSVLESSIKSSILQKSNFHLQLQSQVISNNNNNQNHEDKYISQNPYFNDLSCISHDSENDIFRDCPTYKNGPKSTGSLKSRSKILNQMAYITQKMIPEFQRGIQLISQAEDKVLDFRVEQSEQPVRISSQQLGLGEIMINYQQDKHPIELRPDLWNVQSVRQMEFYYYKLRSKQQPVPLQFYMNQSETTNFKIFVSSVNPFPTKFSCDQIISVKGWKYKIDESKSFHCEYIYISMVCDLDTQVKLKFQFGSRLMKRPQTIQRKIEPSSLFIRSVHHQQLERMNSDLLNAQVEEIKKRRKEKLRQRGMFKDLIKENIQKVVEYNEEHQKSFRELKKTQGDNRIEDVLCRKKLMSQNEQQRKEYQQKTKELSKIIRQRHNFVQSKKYLKNRVCTHWFNNIYMSLICTKIYFMFQHKIEAQNQSTLMAFQVKRIMKRIHKRLSAIKGVTIYDRVFFDVKLVLQGFCKAVYQKQVKLHIEKIVPHLKIRAEIYHLKSQVLITNLKISKIRVMFLEFLLRYKSYKQMLHNLWNKYFKEQFLKYYNGLDQSKKGIYQPYIIQLQNQDNFIQLQSIFIETYSKDKLRKYLKDMSIYAKYLKKLRSQKYNKTKRRDFTNDQVLHSPRMFQLPTYDEVLEYLPLLVVNTNKLDSRTSQQIQQSQMPLIQESQEKKKKQAKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VS77</accession>
    <name>A0A8S1VS77_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Kelch motif family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0700008</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000070">
        <property type="protein sequence ID" value="CAD8178592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VS77"/>
    <dbReference type="OrthoDB" id="283188at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093:SF18">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8178592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="589" mass="68937" checksum="ED75D940283807F0" modified="2022-10-12" version="1">MIERNRVSSQQKLPTLDLSSTNRGTVDRLKVNITVQSGDAQFISFRSLNEQNPKFPLPHNSVDSSSNLNSSIQNDLNQNKKIVQLLGNTLYRQKYEYQEVQETLIKKMRTMTRMQKYVKRIPHKVEPFQNFEKIAQTSQQQVKKKVSFPMFYNGAFFTKLQHQWIPTVRENTMAADYQKRIYIFGGIGSEVFGDMIEYEIQFGKFRELKQKGDIPLNRYGHCLHCIGYPMLLSTQFSYDNQQQNSINGKQLIIYGGEMQFNSALKIRENLSDTRIYDIETETWQLLKPNIECIPESRRSFGSCIIGRGLLIMGGIRSRFQVFNDLHYLNLSLCKWTPLETQKNPFIKGLAFQQLVCVYNKPQYIFEKRETRSKQLKSYDYEGVYCFGGCFIDENKNTQYYSNFLAILKFDQPFNNWIIPEITGKPPLSRIQHTASYIQDMNIIIIFGGRDDQKNNPYFNDLFAFKILDREWIQLDIFGSIPRPRAAHTAVSYRSQILYFGGVNLNGYVEFSVNIAEFNQNQIRQLAAEYRPNDQELEREEQQPQSIINKQYFSLHSITPVQPLKKLTKAEELDLQIKQLSFQNFPKHLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VV05</accession>
    <name>A0A8S1VV05_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0730141</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000073">
        <property type="protein sequence ID" value="CAD8180073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VV05"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8180073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="107" mass="12654" checksum="D4B0E75BD09E85B7" modified="2022-10-12" version="1">MTIFTQKWNHLQIIQRGYCGLIQSMFQSPKDSIFYKCAFDQYLLDTHRGNKVIVLNFKFQTEYGEIFYNQGTILQCSRGNQIILLNCNFLSWNDIILNINQWLCSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VVU9</accession>
    <name>A0A8S1VVU9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">isocitrate dehydrogenase (NADP(+))</fullName>
            <ecNumber evidence="4">1.1.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0750025</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.42" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDO010000075">
        <property type="protein sequence ID" value="CAD8180777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VVU9"/>
    <dbReference type="OrthoDB" id="297722at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004450">
        <property type="term" value="F:isocitrate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006739">
        <property type="term" value="P:NADP+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004790">
        <property type="entry name" value="Isocitrate_DH_NADP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006156">
        <property type="entry name" value="PRK08299.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11822:SF21">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NADP], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11822">
        <property type="entry name" value="NADP-SPECIFIC ISOCITRATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0464">Manganese</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="11">
        <location>
            <begin position="1"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8180777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="231" mass="26324" checksum="8044F4E2D61E576C" modified="2022-10-12" version="1">MAMYNTDESITAFAHACFQHAIELGYPLYLSTKNTILKVYDGRFKDIFQHLYDTKYKPDFDAKKIWYEHRLIDDMVAYMIKSEGGFVWACKNYDGDVQSDTLAQGFGSLGLMTSVLVSPDGCVEAEAAHGTVTRHYRLHQQGKETSTNSIASIYAWTRGLLHRAKLDNNKELERFCNTLEASIIEAVEKGFMTKDLAICVHNTMNVPRDQYLNTLEFIQKVAEILKVNLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W029</accession>
    <name>A0A8S1W029_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AP180 N-terminal homology (ANTH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0760168</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000076">
        <property type="protein sequence ID" value="CAD8181492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W029"/>
    <dbReference type="OrthoDB" id="296218at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045192">
        <property type="entry name" value="AP180-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951">
        <property type="entry name" value="CLATHRIN ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951:SF108">
        <property type="entry name" value="ENTH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="AP180 N-terminal homology (ANTH)" evidence="2">
        <location>
            <begin position="120"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8181492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="517" mass="61210" checksum="E468B623257D254D" modified="2022-10-12" version="1">MESNKVQQMEKDGLIRAMIIKKYILNYFIYKMITNLVRNIVQFGGTSTFEKARLSATYKGDYYYPNESDIEAIMLTLSGKDPETRPQVGFTTILAKLEQSQSKIYWNYIIKNLIILDRCIEYRFFVSDIADMNVNYVENYVERDQKYKSLSMDSFIRKYYLYLKKKAALYSIKKSSFNTPKEEKKNYFKQQSAENIFEEFNKGQQLFDQILDISIHDKNGLLKYRLNQYVYQLLLFTMLNLYYNSYILIVILIERLLKMPINEVKQFKDIYNHFLKNNEVLVNFINTRLYIKGFSDINTSEIARIDTRIIGAIEEYVRIAEKSQNSIGLSQDIQISADLIDEELIFNQVEIIRDSISQRDAQAEPNRAVGHKLSNIQYTVQKLISFESKLKDYQHKYIKKYPQSAFNTDGFKEDLFKSFANNYSTEYSVIDPKLFKTLSYQNERESSQPHSSIESEYHDDGIKIEFIQPINDFDENLFFSIKDIFCFEVPKIIDNIEQFPNADEEDFPDYKDEEDNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W1N4</accession>
    <name>A0A8S1W1N4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0800054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W1N4"/>
    <dbReference type="OrthoDB" id="304507at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8183051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="568" mass="67246" checksum="48B4599D3EB169B0" modified="2022-10-12" version="1">MNKYSLRFDNPKMEELYFQDQYEELFNFYTWACCLGTISAICVFTMEFIVPFGFGFWKYTLIIIPLGFGFSHKLIKMFPQYFNYVLAMMNISLGGLIFILLYFFSNSNLLFLAGQTVAMIQYSLLLGSNIITNILVLLINQIGLMILSSIVQNYFNSLQLMFILALVLVLKSIWSSEKMKRSFFLLKYQNIQLHKQLDNVFQLKILRCKFDKKLNQIKLININKQAEKIIQDQKQFIQFIRQYTIAQIRSLSQPIQMFTSQRHIQRTQTLEQVLYSMLVGDDKEKSSEIYSEVTKIAFRICIYKIIEKNQVHLIVLLQEDSEYQKILKLNKEVKKKNRNQKLLMTCLENAKKSLQILKYLTIEIIEKKQKMHQNSQMNIKIIKILSISQSILFKSVHGYYNVLILNKMQQQLQEIIQFDIEMLSNDVRKLRSVFCNIKQLPINYEGDNLKCESNYVLTLQLILNLLQDSFCDRTYENVLIKTQLQQQEKTKVINYKVYLFINEKTERVCIPLNRIMINQFSQLKKINKKIMEIIGWSNLITITQGTNFDLIEFCLLHSLKEFKEAMKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W2G6</accession>
    <name>A0A8S1W2G6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0780129</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W2G6"/>
    <dbReference type="OrthoDB" id="293967at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053083">
        <property type="entry name" value="TF_kinase-domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44305">
        <property type="entry name" value="SI:DKEY-192D15.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44305:SF24">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE C03B1.5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8182309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="391" mass="46237" checksum="031483E607DFEC9A" modified="2022-10-12" version="1">MGNSQDTQITNDLRYGKVNKFTYENSLIIQKVIDYKNAPDQLANCIHRCQLSQSINHKNIIKLHKYEVDQLSVLIYFEHYDYDLQQDINKRKLNNNYYQEHELLQMFDQFVSALAYLQQQQIFHDDIRPQNIFLTPNDTIKLNQQWVIDNTQNQQRYYAPEQIQPQPSNVQPNPFKSTSYSLGITMLHLMTLTSMEIVYLKNQVNQSLVNELLRCTNYSNKLINKIQHLLFQSPHQRADYMYQVNKQANLQQKLLSSPPKTITSSQRYVYRTSQSPLTRTIQPRHSHSPFTFRRIAQLQTPIQQVTPQQIAIIQSSPRQIVKTSSPIKVIKQQEQQQQQQQQLMFTPNQSLKTKEMLQKLDEVTPKTDLDYRIQKALKQTNETLKKNYMLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W3Q1</accession>
    <name>A0A8S1W3Q1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0800174</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W3Q1"/>
    <dbReference type="OrthoDB" id="293134at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8183287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="132" mass="15226" checksum="DA44FF1EDAA58712" modified="2022-10-12" version="1">MKILDEIKKQVEMEMQKIKKVQPTRIHQPLNSQLKKDLIILINKGVQLKDAARELKITYHEAKIAYNEYRRKALSSQSETESTILDIRTAGVSLLRVPPHHFIVQSVVENTITSVRKLYNVIVQNPILNSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W478</accession>
    <name>A0A8S1W478_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Leucine Rich Repeat family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0840215</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8185188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W478"/>
    <dbReference type="OrthoDB" id="291766at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052394">
        <property type="entry name" value="LRR-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="451"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8185188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1126" mass="132715" checksum="3B588267575E232A" modified="2022-10-12" version="1">MKLNISGTSINTKSSLNTKQDQNVKNLKQNDDYLFFDLTEFQTSDSNTPLSIQLKETNLTNQQQTKHFFPSKPILSENKLPPLNSRYHLVQEISKEQIIKKKLFKENYSVISFPSTQSLFSSKSQSIDYQKLGQQIINTPNSQDYYKFYKHSKKCLDVQTFNETPNNIYVNLNKAMEQQQCMPRKMQIIDNHNKKEISVAGQLSSQQYIKMFAAGLGSSTFNQLERLNISSNNLNNEDLEIVLDQLPKSVQYLDLKNNKISKNGCLFLQKLLLKQHQFVIELNLENNQIGDQGLKYLIGSLQNNKCLRVLNLSHNQIQDQVMEPFGKMMKKNNIIQELYLHYNKITFKGGIFLFKGLSKNSVLKVLDISFNKLGGNKDCTNEICNLIGRPHPELIHLDLSHNDFKDIDSKKIANELEFNKIIYGFHFEGNSNYEINHKGYLKEQKNKKLVTESQQDDINIEEQDKKQLELQMQQMEQQTQKKKNEFIRIQSVDIVNLQLESCWICQGWVEIMFQWKPHKSGYLDEDPIFIHLEFEDYQPSLMQKTNDGYFTLYRMCPPNQTIRYFFSNPVQNVQVYAKDQKTIQTQIHSQLRNFGVQMEYSNGKVLQSQPLHTVNMMQTSQSTPIFDKKKHYLANIQCKPREPEKIVEFDVELAKKRKWTLDNSIFKDFDEDTEAHLEQCLEADLENSKILKHLMNNNEINPLKEQLKKYYKYIISCYKYLASQQCEQDFPRINIQTIQGFYYQSKNNQLIKQVDWEICLISTIVIKELKSQYIQERALVRHQFLELLIRLAKETYVKQGNCSSIAQGFDKMMQAQTGQFGFILDYGCAQQWRDERYWTQIMDMTIRLKLPFLKQLFSYTSSFSNKPHAKEKYVSFQDFRMLLQNSDFFPNLINEQQLYFIYLQSIMMQVDEITQKRHFEMQFIEFIEAIARVAEFISPNSNNYQIKQLNAKQRRTLPLYIKFEGLLYILFYRIKRFDVDINVIEKSVIKSHEIKRLGIFIEDNYSSSSSGEEDNEVSNIRLSYTDPQNIINRMLFDDECRVSLKDTMSNQSSGKKRKNSLQKQDSITLKRSLLRRNTFQNQLQQIKIAEIENEMAELAEYEESTPLNKLRIQNRKITRSKYDIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W9S2</accession>
    <name>A0A8S1W9S2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0850163</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W9S2"/>
    <dbReference type="OrthoDB" id="293577at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8185573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="528" mass="63289" checksum="6C031ECCFB33B275" modified="2022-10-12" version="1">MRHLSMSQSQMTRSKIYTSSHPESQFLQLDNDILEIYDEFEKNYNTIQEIDKTNTKIETKQKIIEKPKIIQQQKKLLKTFDGLQQSDLEIEYINRIQIVRFRFYYFRIKIKGKQSPLQILLNFPSKITSTQYKLFVSTTIEFPNKFNADQSVQSRFVKINSKQNSKLFFDEYVYLTFYSEFDAIIGMQITFGEQQTASNGQKLVQSMINFDTFPERRTIHPKDKILQNIEMAKTKMFSKLDEMLKAKHRNTKSQETIQRSKLIQKEVRQTRLIKLLVKDQIIQFREVEKVHQKELQLQIFVKKNWILITTIFNICRQIYRKLEKVRRKNKIAARANLLVWQLHTKALIEVKKYGDNPFIRSLSKSSIVLSTFTSYIQEKCITKSERIISQFLVDIILYQTFINKHIQLISKIKCIQRNYRKLRLVRKNFKDKFIKAFKEKLQILYQGRGSDQKPRINIEQHIITKLFEEYSAKIKHRWIDSRRAINVLSVFKKSFSVQRQEIKLYSLPDEAEMQLIIQQYYKLKKQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WA28</accession>
    <name>A0A8S1WA28_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0880035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8186884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WA28"/>
    <dbReference type="OrthoDB" id="296306at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8186884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="456" mass="54350" checksum="2FAB47165D49C311" modified="2022-10-12" version="1">MTPIKLGLRLAFLLLFNWTLLLFYFAYITQDQEFSINETKLFINSIFEHHIYESNIKDQNNIDIQIEMSEEEEFEYTEENEREIQFIDDPKGDLIAVGDDDIDTNYDYTIQAIKDINPIKQSILVTNEPIRQQTQLNILKDNNYCELNNLYVITHHQLPINIITDYPNDNIITFALNAAGKDLLPDVILESNKLYHLPFNTTIFFMENLNFHTYFEIGSQFLCNFQAYNHIPGIEILFKKSSLYQILQKTEVPIPETFIMNNITQCLEFFKQYKNLNESEYLIKNHIKRQISQQTLEHNFIRRKYQDGKNCGIVIDDLIIQKKVENRQKRFFAMLLISLVDPLQYQIFDGFYYKIQKGVPLDHQRYNETISKMINNQIEQFIHKIPILKDNRFFSLLRMEFIEADQIYLMSITSNIDKDTQIKDYLTSLIVSTLQLINFQQIQRLSLYQIENHYQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WCA4</accession>
    <name>A0A8S1WCA4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0870243</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000087">
        <property type="protein sequence ID" value="CAD8186771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WCA4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8186771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="112" mass="13426" checksum="02888864757C3293" modified="2022-10-12" version="1">MQPKFQNLVLVTSVKFMVSFWKSTQLFKAIGVQIHDCYHHILNLDRKDKIQELELPPYEFFCEKTNHQLQIFSLWQYLAHVVGINILLFLLSNRRYRIIEQTISFESQTSSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WDW7</accession>
    <name>A0A8S1WDW7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0820147</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000082">
        <property type="protein sequence ID" value="CAD8184116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WDW7"/>
    <dbReference type="OrthoDB" id="305156at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8184116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="331" mass="38881" checksum="5DB087EC104E84CC" modified="2022-10-12" version="1">MKKGRKPLDEQIKIKRKLEQLWKGNFLNLELLEEFQLLNINLPQSKQIFTKDFILQYQKQKNYRCVKIVTSNLLIDCRTIRQKEIQKENEVQNQQVNENQQQSIQVFNVDNRPQNQNIQLPPENNNIELIDQPSIIEILPQQQSQSKENILKPSKLNIQSNNQSIIEIQPIPVTIIKKQLIIEDNNQNSNSFLESPQSKTQNPKLQDKIPKYLLYEPSPPNSPSLNFSKSLSQSFVNQLMDESFFGDEQLKLSESLFGNIPRIIQDPSPNQYDQTFTMLKAKIHDIQQSQFEQLLIAYNHNFLHIQKIILNDINQIKLLISKYQKKKPRII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WM62</accession>
    <name>A0A8S1WM62_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0940158</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="313046at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035774341" evidence="2">
        <location>
            <begin position="18"/>
            <end position="2025"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="944"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1134"/>
            <end position="1227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1255"/>
            <end position="1289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1823"/>
            <end position="1847"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1891"/>
            <end position="1911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="197"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="347"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="367"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="380"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="403"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="440"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="495"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="840"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="852"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="866"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="950"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="967"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="987"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1054"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1153"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1191"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1278"/>
            <end position="1289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1892"/>
            <end position="1906"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8189701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2025" mass="231656" checksum="18E1D367A99D3D22" modified="2022-10-12" version="1">MTLIYIIVFYALNQVLGRFTTLNEALLFNPITLYVPECVEQPKFDNIIISIRYWIEILQTDDCDLQTDLETLKQIKATLPESNLFLSSFIQKDQFKIHGLMQITDDICTDAQNQPGYIIDLLKKELQSIIDSLEEILTTPNKQRKISALNSQIEEVQTYQEFCFQKEERQRRKAQEAINQQAQNIKNEVGYIDDAYGTPSTSNSSQGSTTTSSSSQQSNNQQQGTNIQGTQQPINCVDPQTSNQQQNQQGSNTTTTTQQTTTIQNQPPSTVSQSQSTQTSTTDSPTQYQPQPTSQTVPSQQFQNPQPQPQQQSQTQDFQQVPQQNAPPNSPSQNPIEDPTRTQQQAYQPIQNSPSPSSQPSIQPQQQFPPPPQQYPTQPPTSSGMQTNPPQSQPPQIQQNPPCDIPKPTQEPPQNPPQTPNIPPPSTQEPREVSPQTSPQIPPETPTQVPPQAPPQAPPQIPPQPPAQPPSEIPPVQPVQPPLPPQQPVDCSKPPIEPPVQPPVQPPKPPVEPPKPPVEPPQPTVEPPQPPVEPPQPPVEPPKPPVEPPKPPVEPPQPPVEPPKPPVEPPQPPVEPPKPPVEPPQPPVEPPKPPVEPPQPPVEPPKPPVEPPQPPVEPPKPPVEPPQPPVEPPKPPVEPPQPPVEPPKPPVEPPQPPVEPPKPPVEPPQPPVEPPKPPVEPPQPPVEPPKPPVEPPQPPVEPPKPPVEPPQPPVEPPKPPVEPPQPPVEPPKPPVEPPQPPVEPPKPPVEPPQPPVEPPKPPVEPPQPPVEPPKPPVEPPQPPVEPPKPPVEPPQPPVEPPKPPVEPPQPPVEPPKPPVEPPQPPVEPPKPPVQPPQPPVEASQPTQQPTEIPKPPTEPPKAPEQLKTNITVNGNRTTEVQDLIKQLQEGKMENVSINITVYQNSNTTTIGSQNGELLTILEALLSTQPACSTPAQPVIVVVQQDDDDCKEPVKPKPDENPIPIVQPEEEEEEEELYPEMSFPEPTTEPEPEPEEIPQPIPEEEPEPLPEEEEPEPEPIPEEESEPIPEEESEPIPEEPTNVNTTKPAEPLPLPEEEEAEEYIPEEEIPEEIIPVDTKNTTKPSEPLPIPEEEPVEEEIIEPIPIEDPADTFNQTKVVDPLPIPEEEYFEEEIIVPDEPFVNQTKPSEPLEIPYEEEEEEEEVEEEEEEEEPVKIVVVKNDTKQAEPVPIPEEEPEEQIPEEIIIVDDPKNTTKTSQPLPIPDEGYEETIYEEYYEEYYEEYYEEIIITPAKNTTKSAAPLPIPDDDDDDWGQMAFGDESDEGDEGEWETYWDGSQYVKRKRSTTVIEYEYYWDVDTQSYKTRVKGSGPNEDDYELYWDGSRYLKRKKGSQTYDQGTKPSGTYTYDSYVYEQYWDEGSNSYQWRRYRVGSTSYESGIYEYYWDESSQSYKFKQSSTSSQSSQTYNGYTYEKYWDQSSNSYKWRRKQIGSSTWESGDYEYYWDESSQSYKFKQSSSSSQTTYTYNGYIYEKYWDQSSNSYKWRRKQTGSSSWETGDYEYYWDESSQSYKFKQSSSSSQTSYTYNGYIYEKYWDQSSNSYKWRRKQTGSSTWESGDYEYYWDESSWSYKFKQSGSSTSSSSQTSYTYNGYTYEQYWDQNSNSNKWRRKQTGSSTWETGEYEYYWDSNQQAYLWRLKQQQQNEQNDYEWQWDWETGSYQWRRKANSYDSNEYEQYWDSSSQSYQWRKKSSTSYNYDDGQYEYYWDVDTQSYQTRKKSSTSTEIEYEYYWDYDTQSYQIRQKGSSQQSGGYSTTSSSSTTYYEQYWDADSQSYKWRVVSNDQIAQQGTYDDGQYEYYWDASSNSYKTRLKSSSSSSSTKYSSNDGEETYSYSTTYNVSSSTDYWEEYYVNEEGEMVTLNLGSQLLNKNDALQSDNQTHEKWISQDTKQTDDSLEAQDNSANQAKFIYKTNQVWEKDDEQIYNSYNLRSRNIKNAAKMKNVNHNDNQKPQQKKQIIGTSFNFRTIPQVQDKVEKQQQLKNQPEFIDKKETSEKQEKISMLKKIMNIFSHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WNS7</accession>
    <name>A0A8S1WNS7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0960067</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000096">
        <property type="protein sequence ID" value="CAD8190337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WNS7"/>
    <dbReference type="OrthoDB" id="270720at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8190337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="356" mass="41590" checksum="5DE21F1221202EF4" modified="2022-10-12" version="1">MGVCCTHSHEKFENFIPPELIKEDNKEYDLFALKIQKHYKGYKARKLYHKMKIKTEESPVVHHPKPEIAQKLSQTPTDYALTVQKALYKLGPFRYNDFINSDDPNKRSTNFQFTDEDKKLPFLEPHRLIDGAIYIGQWKNGMKWGKGKQIWPDGSVYEGFWQQNKACGIGRLIHQNGDVYIGDWLDDKCHGFGVYTHNDGTIYKGNWIEDKQEGDGSEEYPDGTIYKGQFLNSMKHGEGECFYSNKNYYKGEFQYNFLHGQGKFVWSDGKEYEGAWENNQMHGYGEFKWPDGRSYKGQYKNDLKDGEGEFIIDENTRYKGSWKNGKQHGDGVLVVNDIIRPGVWCEGIRIQWKDKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WP02</accession>
    <name>A0A8S1WP02_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0960074</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000096">
        <property type="protein sequence ID" value="CAD8190351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WP02"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004771">
        <property type="term" value="F:sterol ester esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002168">
        <property type="entry name" value="Lipase_GDXG_HIS_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23025:SF3">
        <property type="entry name" value="HORMONE-SENSITIVE LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23025">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01173">
        <property type="entry name" value="LIPASE_GDXG_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="3">
        <location>
            <begin position="440"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8190351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="680" mass="78039" checksum="DEC63F0C9B4C63AF" modified="2022-10-12" version="1">MQTIEDQINSCMSMANLTKDISLELQTEEQSIEIQSIEIKQQLNGDPVQVKYSENQLQEENIMKSQWQVINKVWNCSIFNQEVISKELYQNLSETYYLLNKLHQKAIDLTKMYNEVADFLPRFFQAIKITFIGILQLEQIGLITNDKDIMSKSINEEYNINLNTKKFNQKAICILLEFVIAHVLSHISVLFETIQVRREKQELRSLVIYLSKLQTCLEQLPGAAKLDNDDIFCYNRTDPRWQGLNSIIKEVVLGTREQISNSYGKVAEGILLANAMISKGSEFNNETAKNVMQGFGALYYLVAKKDAKLKADHFMAEPNKNLVFKAWNLGESGFLSKLLPLLFPMITYNKKIYIPQLFKRLVQDNILRQYKEDKINRIQNDCGECINTNLPKCPELFTKNKEKIPVRVLCHYNLQEIQTDSFMQQSLKLLQGQKVFDKIVIHIHGGGFVSMSSRSHQTYTRKWAKNLQVPIFSIDYRKAPEDPYPFGLDDCWQAYMFIMNYIDKYFNIKPKKVVLVGDSAGGNLVAALTIQIIKSGARIPDGILMAYPALCLDIKQFTPSLLISLNDPLLHHTVLKLCISSYVPEQFDAKLDPLMSPSIASDEILVRFPPTRIVLGTNDPLHDESFRLANKLLKLGKDVKIIEYKWMPHGFLSFDVIQGMKEAEQTVLDAQNIIQNLLTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WPI9</accession>
    <name>A0A8S1WPI9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0970048</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000097">
        <property type="protein sequence ID" value="CAD8190650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WPI9"/>
    <dbReference type="OrthoDB" id="10255449at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8190650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="689" mass="82452" checksum="A1BE5D932C13C4C5" modified="2022-10-12" version="1">MEQKEGKVCLVEVPIEKTFLFQDQIIIDKNLSTLRDGLSYRFSQNFINPQQKIMIVCPPTYALKELDTKKNNNKLFDGKGYIKAIPKAEYPFLIAQQSGMRSSIIQWNGKNYRLKGCGNGNDGFTLRKFEGDIDNLYEIRGCCFDHTVKRELFMNRKVEQILKKYNIETGNISIGYWQYGDIESINQNLEKNLQNQISLIPKYCGIYETIGDFRLGTHLLQGLRFIVNSNISNWCLDDIRRLITVQSRIVENRKSYNGNSWDLNNTQYRERPDGTIFKEDDYKNNRVFDWEKEQDNISITQNLTGKTILQNISILYQRIGYEIGKIKRIFIDNDINWGYFRDHSKFYYCKQFCQFIYLIKCFYFKQLFKQYMKNIQYQEFTFLKNLMSWQYIKVNQDETVFQKRSIQKQYYLYSCLIFIFQGMKHIFQYFQMDKLKSKVWTIKRLQSWKLIFILQLKSDRTYNQVFLQTWRAGARSIYQNNNNNKLKGYYHQNSIEAILVLVKSVSQVMFQCPLLTFIINLLQSISFNNPHNYTTSIHTQKCSICFLIQNIDFYFYLFIDLSRLISKIFQNLLQVFSCQEFPSEQYYILLQPQVPTLHNKQSKNKLEQISGEKLKKNNICYYFIFIFNLWIQKKQAKRRLKLFQQISYLSTQSIVGQFSRDFLITLKMPKCILEWSRKESQKSQEQFQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WQB9</accession>
    <name>A0A8S1WQB9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0990018</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000099">
        <property type="protein sequence ID" value="CAD8191422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WQB9"/>
    <dbReference type="OrthoDB" id="284308at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8191422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="141" mass="16364" checksum="CF8B597344F21D68" modified="2022-10-12" version="1">MTHQMTKLYTQDGTGRDSYIFFNNGGFYPAQSRFLVNRMAESWAYGSPSSVRLGGSIEKRQFYFSDGTGRDTYIAKTVQNKAQLSPDFQETLRKYKSQQIPTKYPYKLPPIKQAVQYEKSVKQHEFTKRLAAPKKRIEDND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WS63</accession>
    <name>A0A8S1WS63_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Trimethylguanosine synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0980060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000098">
        <property type="protein sequence ID" value="CAD8191059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WS63"/>
    <dbReference type="OrthoDB" id="194443at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071164">
        <property type="term" value="F:RNA cap trimethylguanosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019012">
        <property type="entry name" value="RNA_cap_Gua-N2-MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14741">
        <property type="entry name" value="S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14741:SF32">
        <property type="entry name" value="TRIMETHYLGUANOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09445">
        <property type="entry name" value="Methyltransf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8191059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="494" mass="57165" checksum="9BBCA9AD60F036D7" modified="2022-10-12" version="1">MSSKKKNTQKNDDGYDDDRCFCGWIKKKSSKNVNIEQSKISVNKQHRNSLYKSALDASNHNGISQFNQLNSTNSETQRKNSEISPFVSSFVVPIFENNYPLNQLFQLKNNQSGDTIFAKITSTQIDVSKSMGFNEDICSNQRIIDQISKYIASRIKDQINSLAEIGCGEGGNTVQFAKYLDFVIAIDKCTEACQKTRKNCDQYTQNQDFPNPKVEIINCDIFKFKKRLPFDSIFINPTINTENTQICKDLLKDCQPNLQQLLMMISDQIENLIIQLPAQIDYSQLPLLLNINQQYQNNTQHKYQSLVTQHKQQQSFIAHCSLEIEKIYVNGIHIQNIIYYGKIANISREELRSILIMQLQNQEVKPEAIQNLITKLKDKIGSLEVVYELLKAQSFKYNIDKFLEVVCDKYKLDYGEYAFILYQKLSKEFSQNTFNQHYKLRDSNTEQQESECAYSPKFSYNLNGLQIDDTNSIDENEDIEVLGLSQDDRKCKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WV30</accession>
    <name>A0A8S1WV30_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1000110</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000100">
        <property type="protein sequence ID" value="CAD8191969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WV30"/>
    <dbReference type="OrthoDB" id="75724at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46818:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_125, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46818">
        <property type="entry name" value="DOMAIN-CONTAINING PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="120"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="343"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="424"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="424"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8191969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="481" mass="57022" checksum="A7CE054EDA2CB302" modified="2022-10-12" version="1">MIDYSYLAPPKEANDIVHSNHYIKHQEQEQGVRKIYETVVYDQFEIEKVKELKEEIKKQKIILSSDWKESDYLRISYAGRFDKKTVIKKLQQHIAWRCNKIYHEINSHSEQFLKEGICYLLGRDKQYRPIVILNAHLIDQKKHDKEQIFQALSFLLGIVKKNMFVPGKVETWNFILETNNLGGQGLQQKVLEILIDNLSTNFSGYLERMFILNPSSGINFLWQQIQTFLDQQTINKIALLQQKEIKQLKEFIEPNQLEEKFGGNQPNLIKFWPPYNLDLSDGYTKQDNKSTQKQELKLQNIKIEEEEENDPYDQVEFNLNDFMKQQQQIYYCITNKMEQQIQEDDKVEIQQKEQSQPDSQQESKHQHNISQQNHQKPILDEPEQPELQQQKFTHEEEKKQNILQNNEIKESTEQVILTERQKNYQQQGNDTEQNKLQSHDEQGFNSTGNKLNEESNGENIHIQRLDPVNNDKGCCSKCEIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WZ98</accession>
    <name>A0A8S1WZ98_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Leucine Rich Repeat family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1050074</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000105">
        <property type="protein sequence ID" value="CAD8193850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WZ98"/>
    <dbReference type="OrthoDB" id="290471at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052116">
        <property type="entry name" value="Centro_Cilium_Assembly"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23170:SF3">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23170">
        <property type="entry name" value="NY-REN-58 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="217"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="407"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8193850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="585" mass="68371" checksum="E151682CF0656BFE" modified="2022-10-12" version="1">MKLQKALEAQKAPNLILSDQILNEEGCVVVAQFLAQNTQFINIELRGCNINGRGFERICRSISPDARTLIAEWNAIGGGVSALCDLLMNPSYQLQLVDLKNNRIQADHCARICQMLKQNTSLQSLNLKWNEIGEKGAQYLLEGLNGNRNLKFLDVSCNKIPEGLATQIRERIEQNRSSDLMAKNSTGIKESQRNVYQPVKTIPKTAATTNMPDRAVKKNDAIRVAQLKEQIDQELRQNRLMNEELTNRMNALRQEILSKEQAVSMAKRHFEEEVTRKSIIEAEYTQVKRQFDELGKASDYRQQEVMNEYVAKNKHVLALENEYMHELSQIRHDNKQQIDQINANWQPRLDGIQSKFNSLMKANDQYNQQLLRMKEFFLKMRLDHDENVKAVEGRLLIEEKAKYNHQIELLKKKEMETQQTLKRAENESQSIQDDMQRLETNLTRDRQLIEEQIQQEREKVHQTHLQISKRSIKQEKLINNNIVLDHHIDNLIRKIDETFFILNIQKDRGMDDIQQRRLLQERQYALHKTNLQLQHQRILELERLMSSQEGNNIKLRAEYEKLCHIVQGKVQNAVIETCRLNLQLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WZP6</accession>
    <name>A0A8S1WZP6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1070191</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000107">
        <property type="protein sequence ID" value="CAD8194842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WZP6"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8194842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="91" mass="11015" checksum="9A96283DCA00CCB2" modified="2022-10-12" version="1">MVLIMLLELLFRIMLQQFKLPSLMFLYDNLILLFNVMIKLEYGQLGNTKRLFSKIQFRMDHYLRHHAKGEKFIVMVSLRVIMQNLLERNVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X086</accession>
    <name>A0A8S1X086_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1100011</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000110">
        <property type="protein sequence ID" value="CAD8195563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X086"/>
    <dbReference type="OrthoDB" id="10416605at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8195563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="39" mass="4709" checksum="0160CC68436D50ED" modified="2022-10-12" version="1">MDQDGRKGDEMECVLMMVWKIKKINKCIYELKYGNKLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XAH0</accession>
    <name>A0A8S1XAH0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1180006</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000118">
        <property type="protein sequence ID" value="CAD8198196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XAH0"/>
    <dbReference type="OrthoDB" id="295560at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035714146" evidence="1">
        <location>
            <begin position="24"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8198196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="248" mass="28151" checksum="20488297C94B94F4" modified="2022-10-12" version="1">MAFVISFLKILFLKSIIYENCITDNTADCRYTDFSIAGMSGSTLDRCGSQAKNLFMGPYAQLTRVTKTFINIPPNKQMELSIGIWKLDSWDSEGFEIYANDILIQTLLVSAYEGIFTCRNGEFQDQLIPLSLKFQISGTDLTIQLKDNLDQSSWDESWGFRDFILRLAVPCVNFYSECNYTGTLFQICQGEQTKKQTSIPYEIKSILMGPGIIVKFKGPNYFSGVLQEFTTSQPCLNGFKFTKYIKPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XAK5</accession>
    <name>A0A8S1XAK5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Calpain family cysteine protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T1170077</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000117">
        <property type="protein sequence ID" value="CAD8198049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XAK5"/>
    <dbReference type="OrthoDB" id="287147at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00044">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000560">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022684">
        <property type="entry name" value="Calpain_cysteine_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022682">
        <property type="entry name" value="Calpain_domain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183">
        <property type="entry name" value="CALPAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183:SF379">
        <property type="entry name" value="CALPAIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01067">
        <property type="entry name" value="Calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="90"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="185"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="276"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="364"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="Calpain catalytic" evidence="8">
        <location>
            <begin position="483"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="4"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="553"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="724"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="749"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR622684-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8198049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1007" mass="118516" checksum="DA2BF30C4BA3B6A4" modified="2022-10-12" version="1">MKSIDEYKKIVITLKKKVKLLQQEQQDLKRLQQEKVYVYSDRYTAIKQLKKAAKKLGLTTEMFFRAADTKSMEIVTTKRLGEILKKVARLHENYVQELLNIFDVENSGSITKDEYYQTLEMYQINEEYGNNFNRNSYSKFIYFLKREQYDGIIGFIKMDPNKLGYITREMFVAFTQSLNNWQQYLTLREIYSAFQYMDISENDQISREEYLQAFEKLPALPQIQQIQEQKVNILRKVLLIKGVKLSSFVLELMLSIQKPELGITIEVFYNVAKQYLDKKETLAFFKLMDYQQKGSIDYDEIMSFYYMTMSYGDSFEIVFTILARKLQAMNKSIEKYLKQEQIYAETQLTLDQFNQIAQDLFNTPNYEISAKLFEAMDLDNSGIVSAQEIITYTNESLKNYSLLLRQKTQTQTTMLFEDFDSTQIEIANLDIEEQLLFELKKYKTTFSDKDRFENFQYIFTNEISCLVVCLQIVENLKFNKQKYWEDPEFGSNKLDPYGANSIFRIENLPVKGWPDPKDLCWQRISEICNDWVFMDENGANANDVIQSHYLGDCWLISALTIIAGNDQYLVDQNDISKGLYPKLFHFLSKYGIIVIKFNKMFRDVYVVIDDRFCTYNNKLLFAECRSEREWWVQIIEKAYAKLHNCYESLTSGDISQALFDCTGIPCTRYVIDENDNTKDPQEKKQQLLNLFQVGKQTNALMGCSAHGTGQVLLNGEETGLYRGHAYSILHYFHLKDAQSRIHKLIVLRNPWGFGEWKLKWSDYSIEMNENFQLIQAYFNEEIQRCEQNDIEPPDQYNLGDDGIFIMNFKSFQNIFNNLFMSVNLDEQWSVMRIYDSFTKEHLGTPNKNERSQAQYPYNNPRYTIRINHPFPNKKIYIALSQKDGRLDRFAKYPYEGKLVKIIMCLFQLQQNETELTKFQKPVLDSKLLSGRRDIDMEVFSVQNATYVLIPSAHKPNITGDYVISVFFDFPPNGIIIESSTEKQFLLQPKIFSSLMQNPTLLKLFNQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XDG4</accession>
    <name>A0A8S1XDG4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1200024</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000120">
        <property type="protein sequence ID" value="CAD8198831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XDG4"/>
    <dbReference type="OrthoDB" id="9997739at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8198831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="203" mass="24699" checksum="413E31AD4FE3188A" modified="2022-10-12" version="1">MQLSLQTQLFILIQNIINSFFYQQNNCFFSLTLYLVIQIKFKKSLTILNYQQIEQNYLNVLNYHNGDLISSKNKNQFNDHNDGSPFRDEIYLVTSYFGFLMCSLEQSIQINLIQKYELNTLKIWLWDRNNRFYTMEVFIIFENVETRIYENNQAQSILTIKFPAQKVESFRKQMIYKLNQKLSNDYIQYWRQYSQFNFTYNKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XDN9</accession>
    <name>A0A8S1XDN9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T1210104</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="7">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000121">
        <property type="protein sequence ID" value="CAD8199287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XDN9"/>
    <dbReference type="OrthoDB" id="282152at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006363">
        <property type="term" value="P:termination of RNA polymerase I transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10507">
        <property type="entry name" value="Zn-ribbon_RPA12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034004">
        <property type="entry name" value="Zn_ribbon_RPA12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239:SF14">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005586">
        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS-type" evidence="9">
        <location>
            <begin position="75"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8199287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="118" mass="13634" checksum="5E24774091F7A4FB" modified="2022-10-12" version="1">MNLDSAADFCPQCHFMLELPEVMDVIECNRCAYKCSITDYQPKYIISTIKMEPKSWLSNQPQESKLSDQSHRAVIQQACPKCSHEEAYFSTAQLRSADEGSTVFYECVKCQFRYQLNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XES2</accession>
    <name>A0A8S1XES2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1210116</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000121">
        <property type="protein sequence ID" value="CAD8199311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XES2"/>
    <dbReference type="OrthoDB" id="295542at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035791293" evidence="2">
        <location>
            <begin position="17"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="50"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="276"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8199311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="374" mass="43893" checksum="D0FA5658EF75D364" modified="2022-10-12" version="1">MKVIFIIFLSLLIVGCEKNLSTRIESLLQTQLGHTLLSTAQLALQTNTPLDRIIDTLQDLEDKYQKDQKEEDLENREFQQKCDTDLSNLNNEIDDIKRLKVKIQGAIDQLKTSIESKKKILDDKNALRKNLRKGMNEVDVIREYQVKVYEEKRGEVGKIIKTLEEVKLVYAQKLRTNFMELQTDKTKVQPEVFAEVTKQLVSFGKFKSIGKPFQNIIDFMIQMSKNGNNIGVLRNIIDLSESLIRQIDEIWQVERKAEEERVRWYTEYQKFMNTDFKIYDKEIGDLEAQIQSLKDRLAEYQKKMDDLNIRLESKEYTKEDRRNECQQAAYDYTQRRTSRADDRNIVSEVVGLLNVNMRDLKETLAQKMAAGDRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XF07</accession>
    <name>A0A8S1XF07_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1210187</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000121">
        <property type="protein sequence ID" value="CAD8199453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XF07"/>
    <dbReference type="OrthoDB" id="63533at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:004966">
        <property type="entry name" value="Rab_B73 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="197"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="199"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8199453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="223" mass="25026" checksum="B81B8BF2CD935657" modified="2022-10-12" version="1">MNQSQQSQEVYKIILVGDQGVGKSSILLRYTKDLFYRDYNVTIGLEFASKKVTIQDNSLTLQIWDTAGQEAFRSIARSFYRNSAAIIIVFKLTSRESFQSISGWVKDIQENSDEGVVISLVGNMSDLESERTVTTEEATQKAKELNALYFETSAATGSNIDDIFINSLEKVSQQLNPIRTNSEQNVRDSIQLSQKIQSQNQVKQEQNGGKKQKQVESSNKGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XHG1</accession>
    <name>A0A8S1XHG1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1240028</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000124">
        <property type="protein sequence ID" value="CAD8200254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XHG1"/>
    <dbReference type="OrthoDB" id="314008at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8200254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="61" mass="7601" checksum="70BFB17AC9FFE1C9" modified="2022-10-12" version="1">MDQIAAERIQEFEQKSGMKISEFKTENPIKEEIRPEYIERQKRIYRQKMEETRKQLQQQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XKY2</accession>
    <name>A0A8S1XKY2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Insulin-degrading enzyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T1300036</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the peptidase M16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000130">
        <property type="protein sequence ID" value="CAD8202090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XKY2"/>
    <dbReference type="OrthoDB" id="952271at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000079">
        <property type="entry name" value="Coenzyme PQQ synthesis protein F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000012">
        <property type="entry name" value="Protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000108">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050626">
        <property type="entry name" value="Peptidase_M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032632">
        <property type="entry name" value="Peptidase_M16_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054734">
        <property type="entry name" value="PqqF-like_C_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690:SF18">
        <property type="entry name" value="INSULIN-DEGRADING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690">
        <property type="entry name" value="NARDILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16187">
        <property type="entry name" value="Peptidase_M16_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22456">
        <property type="entry name" value="PqqF-like_C_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="10">
        <location>
            <begin position="110"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="11">
        <location>
            <begin position="269"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 middle/third" evidence="12">
        <location>
            <begin position="456"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="Coenzyme PQQ synthesis protein F-like C-terminal lobe" evidence="13">
        <location>
            <begin position="916"/>
            <end position="1014"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF22456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8202090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1111" mass="129613" checksum="8252F8DDB72D7ACC" modified="2022-10-12" version="1">MLKQQREAIERAKLNSTCNTIISTLFVVLVILLILFICDLSGLVQLDKPLQYVSAANAIKDSIPERNFISASQTGTLEPLQGQHIDKVKVIKPIIDHRQYRYLELENHLKVLLIHDPESEMASAAMDVKAGSWNEPDEYPGLAHFCEHMLFIGSQKYPQIDFFDDLMAKGGGSSNAYTEAQNTNYYFEITVNHLGKALDAFAHFFIDPLFNEDAVNKERNAVNSEYEMDVSSEDWKVVNLFALLADPKHPASRFSIGNNDVLAKEGVVEALKKFYQDNYSSNIMSLAVSSRLSLNQMEKLIKVFQKIENKNLIPLQFSGFPYQLGLFGKYKTEKKLILLNWQLSGREQYIHQKPLELIDYLLNNGNLKDYLKEKQLVIEFESSIFIEQNHFVNYIIELILSEQQLEDGKIALEITRVINNYIQQLEEWLKDDKYLEEIFKEQSQISKINFEYMEGSQDISDIAYNLNKYEPVEVLSSSFIMDILDKDIIYKYITELKKTDNLLILIGDDEYQFIDNTFKLNNNKEFLKDKNLDKRSEIYRLEYATQKMDKDSIKIITQKDSKLQKLFNKPQKNLFIPDDLKLVSLCESNKFKLPILINSDQLKPLDKDAKLNLLFNAGQDLQKYSKDQCKKQENIYQKNNYYPILLNKENNQWWKFSTLYKLPNVYGAIFISYTKPLTIRQFTSMRIFNFISEEEVNKQLRQQLTTGYSVELDMSKQIEIKVYGFSEKVRSFFKKICGCINPFKDKTNSFIQFEQTKQESIDFIRAKQSLITSIKDIFQMKLFEQSFQLYLPLILRKDYFNPEQVIQQIPNITEEQMILDIKEILQNSIQSSLLVGNLDQKKAQDLSSELKTCIQDQNTNIHISERLPISVLNLSGKNWIFAKFVESDKGNLNGVTLNYYQIGQRTLENYALMKILQPLLNSQAYNHLRTNLQLGYVVLMKFKQISCIDGALFLVQGNKEQPFKVNQLIEKFLLQFDKYLKKLKNKQFEHLRHSSIVELREKPQTLSEEADRLWGYISSGDYTFEERQVTIERMKLITKDQIVLFFQNIFIKNRSKISLQLYGEGMVSKTLGLKNNKEFDTYIKTNIPNDALLFTEKELTYYNCQNDVSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XNF4</accession>
    <name>A0A8S1XNF4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PPENT_87.1.T1310148</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000131">
        <property type="protein sequence ID" value="CAD8202651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XNF4"/>
    <dbReference type="OrthoDB" id="1413014at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005745">
        <property type="term" value="C:m-AAA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000019">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000003">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000277">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050928">
        <property type="entry name" value="ATP-dep_Zn_Metalloprotease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655:SF2">
        <property type="entry name" value="AFG3 LIKE MATRIX AAA PEPTIDASE SUBUNIT 2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="19">
        <location>
            <begin position="316"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="23"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="679"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="40"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="72"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAD8202651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="749" mass="85652" checksum="DF59AB1F26D17845" modified="2022-10-12" version="1">MLSKLARISHFRLFFAFCDQKPPQGFEKFKRKPKQSNEPSEQKIENIQEEKVQENKQNKKQEENIKRERPIEPQFEQPKQDQQQNINKRFSQEQYKFSKRENTNNFGEKPKPTTPSQDPNDQFKQYLFMFLASVPLAYLVYKAYEEGENYISFNEFQRGYLEKKFVISIQVEKINGEIIAIIYTIEGKKKLKIMDLDYFLQKIDDLKNEQQIPISFQNQDETQETTANITKYASILFSGGLFFIMFQIYKNMTKSLSGMAGDMMGGMSKMKSKQFEQKIKIKFKDVAGQDEAKGEIKEFVDFLKAPNKYKKLGARIPRGALLTGPPGTGKTLLAKACAGEAGVPFFYVSGSEFVEMYVGLGAARVRELFKQAKSKAPSIVFIDEIDAVGKKRNSKGSKNEERDNTLNQLLVEMDGFGTDSTVVVLAATNMRDSLDTALTRPGRFDRNIEITLPDINGRKEIFLVHLKPIKLDPSKTIEEYAKRLATLTPGFSGAEIANLCNEAAILAARQNSKYVTSFHFEQASERVMAGLEKKKFMSEEERKIVAFHESGHAVVSWFLAGGDPLLKLTIIPRSKGSLGYAQYLPNESNLQTMEELKDKICCVLGGRISENYFFKSITTGASDDLKRAYDYANAIITKFGMNETIGQIGYQDDQYSKDYSDKTNEIIDEEMQKLIYQCTQRTEELVKKYEDKIKALAELLLEKESLDLQQIINLLGERPFPPKSNYKAYLDLKKVEQTEIKNEIPQKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XT96</accession>
    <name>A0A8S1XT96_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Macro domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1350039</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000135">
        <property type="protein sequence ID" value="CAD8203802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XT96"/>
    <dbReference type="OrthoDB" id="299302at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro" evidence="1">
        <location>
            <begin position="20"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8203802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="235" mass="27358" checksum="23A8A9D1E5B6B003" modified="2022-10-12" version="1">MGNNICGLDEEKKHVLQPESKIITTRTIKQTTISIIQADIAEDLSDVVVNASHEPAWSYLAKRNKNNSANELTQQMKIGELIKTKSDNVNSLEIYHVRLPYYQDTRDLQLIFQSYQVCLRQKGFNSISFTEQNSPNFSIPKQFHAEVLIRAILQSITDDEIAFEQIKFVSLDQITLKYFAYELMKLLDEFRIPDLYKFRQQQFLNYLQNTEELKNEVYLLSTGITTDIDQFDGQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XUX4</accession>
    <name>A0A8S1XUX4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1390044</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000139">
        <property type="protein sequence ID" value="CAD8204915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XUX4"/>
    <dbReference type="OrthoDB" id="300936at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="303"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8204915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="376" mass="46169" checksum="4941060F6A280548" modified="2022-10-12" version="1">MFKNFMYVEYGQELQCHEKLYQNALKKQQKQKEIDYQKQLQILIDKVNEIEDEPPQQITKDFYDYSMKWKNLVNQKRFQEQYQNEMSLQQKSQQHQIQKLPQNYLSPIDGWKYHAQQYFDKKGSRQQNESSFQPSINEKSKLMEFDEPVEDRLIKYGQQREQKYLFEKQFEKFYEQVQNDQRPTKPNEEVFSRLYNYERKQPQPEVYQLNINKLPPEFEERVKKRREQYSLFENRQTRAQTEITDHEIKVLSQNQFDEFLKRNFCNNFRTQSFKEESIESYPFQPELNKKSLELASKNTDTLIERQEKFLIKKQQTLQQYQEEQQLEKQRQFENEFKQINKPSSKIRVNKPSKSPIRQQNAYNLQTLINKQVPQLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XYE1</accession>
    <name>A0A8S1XYE1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CBM20 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1420117</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000142">
        <property type="protein sequence ID" value="CAD8206005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XYE1"/>
    <dbReference type="OrthoDB" id="292383at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001070">
        <property type="term" value="F:starch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002044">
        <property type="entry name" value="CBM20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15048">
        <property type="entry name" value="STARCH-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15048:SF0">
        <property type="entry name" value="STARCH-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00686">
        <property type="entry name" value="CBM_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01065">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM20" evidence="1">
        <location>
            <begin position="7"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8206005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="217" mass="26055" checksum="9CF74179B5521EA9" modified="2022-10-12" version="1">MKVSQIFFTITKQVHYGEAIYIVFDFQNWDLTKAIKMECLKNDQWTKVIDISCSSFEYKYVIGQYNNIFDQEILWEQGQNRSSENLKILEMNQSKIQFEDVWEKRNLMFFLLDKKLNKKSKKNHEHDILLFGQAKALNSPGRFTKCQLNQNTQLYFIILQLELDEIINPKEVQIYMKVQLKKRYIEIISKSIKLNFRNQPVNICEDILIHSQCYFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XYG7</accession>
    <name>A0A8S1XYG7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1440134</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000144">
        <property type="protein sequence ID" value="CAD8206639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XYG7"/>
    <dbReference type="OrthoDB" id="286653at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045111">
        <property type="entry name" value="Vps41/Vps8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616:SF8">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 8 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8206639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1129" mass="134157" checksum="804C340BEDAA5EFC" modified="2022-10-12" version="1">MSSDQKKQKKEKIKIQPNQNANIEDYIVNNTHSNFQEFFDYKKELCGLPTYMTMNNDLVFVGMEYSYIYVFQQDNDFKQGYKILGKDTQIYRGQVKMIQISNDNQLLLVTYSNNEFVIFSLKSFKEVLQHRYQHLKYAYMVPLSINSDHLLQLRKEQYYEIMIHLENNYLLKVPLEININTLEQKIGIPIYKFGEPCERQKIKENRHAKVIENDYMMPQSSIIQDVSIMDSCYEEFIQCMDDRESTASYGSLLDLSPTKKRKNNFLKQIFSNSTNQKQEQPEETNEYVISTQFYLMAVGFVNKIQVVKIFFGNKLNEEQQKIQLLLNFQRPDAQIYNIVFNQPQAIEEQKTRCSCAWGLGSFKETNKRYVLLIINWGCREYYAFKVMSSDYKIQVIQGGHFYNPVDLTQLREFPLVCNFITNSVFFSVLHNKQNQTVMKLLTTSQFEFGVPFTLNQIEKHFNQEKHIQTLQSGQYNPKFYTFAIDNKQDVFISQNVMDSINDGYQKSNDIILNYKQLHPNLQIIYKNEQCYILNGTELQSIRLKKWDELLYEQSDNNEWDKCFQTAVDIHRGYLTLLCNIPENDVQRHFCIKETCCKLGLQYMLSQLPNKQLKNSQSVLTIMHFLIKTQNEEYLFESIEDLMVGCGYRNVFYDYLRELLQQHQVNIPYQHQLKVLKMFTEMDDKETCTKLIMSIQNIQRYDPKGLIDFCLEKDLIEPMMHICSQINDFLTPYLRIITLLSILSNKNSLTEEENKSITTLNLDQCKVCLFSFLSFCFTGQNQSNPESFFTDRQFKAMFKDLFEYLFDLENILKLFEIDYIKTLEVFLQVFSERIQENFKQFINEGKEINIELSNNTINGFMPEILNIEQTDNHLKILSKIYVLIKMGEQKYYVRLNNAKVIDEYMHYSCAFSTNVFAICPFHFNINHIMLNLINYMDALRQQQSTLFDITWILRGRVQKDLPAELVKNEFLHKVMLKVEKQFDGQYKLLEELRLKAQENNWIWMEAFCLIKMKRLPEALYLYMRNPDPLLSERVFTALAVQLRQKQQDEFIQDWIYKNLALLAAENSHKLFQLLYTYKKDDLQEVFKELKQVGIIQVDLKIKNQHQMDLLGGFIEYLKPRMQIDENLLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y1R2</accession>
    <name>A0A8S1Y1R2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mitochondrial import inner membrane translocase subunit TIM16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1480081</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000148">
        <property type="protein sequence ID" value="CAD8207716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y1R2"/>
    <dbReference type="OrthoDB" id="10262892at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005341">
        <property type="entry name" value="Tim16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12388">
        <property type="entry name" value="MITOCHONDRIA ASSOCIATED GRANULOCYTE MACROPHAGE CSF SIGNALING MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12388:SF0">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03656">
        <property type="entry name" value="Pam16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="128"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8207716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="17992" checksum="570E4AF301B2A3FC" modified="2022-10-12" version="1">MVKRLIIKLIIEVGSTVAKSFMKAYQQTAKQSGGKAGNPFTEFLNQTMQAANLTHKPMTREEAFKILQLVPEKASPEDIIRVYWRQFHKNDPVKGGSFYLQSMLHNAKCELMKDFADVNEKEILEKIKKEGESQKGDEEGQKDEAKVEDKNEEKKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y2Q9</accession>
    <name>A0A8S1Y2Q9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1470081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000147">
        <property type="protein sequence ID" value="CAD8207427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y2Q9"/>
    <dbReference type="OrthoDB" id="294378at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8207427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="219" mass="25398" checksum="C4C02B49A448E52C" modified="2022-10-12" version="1">MKYQYKRILDSNKNYFKKRSGNNNRMIFIYKESQVINLKKLAKEEIQCYMGSCCQVSQVAREQYVFPDGGVYTGEMKDGIPHGVGAISFDNGSSFEGHFENGVKCGKGVYRWSDNSYYDGEFQNDAFNGYGEFYWSNGKWYKGQWVQGNMQGEGQFYTDGKTYKGEFENDKRNGKGEQIWPNRQKYIGEWKNGKMHGRGQLIEPNGRVIEGEWVKGKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y2V8</accession>
    <name>A0A8S1Y2V8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1420126</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000142">
        <property type="protein sequence ID" value="CAD8206014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y2V8"/>
    <dbReference type="OrthoDB" id="295442at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="63"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8206014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="223" mass="27016" checksum="EDD53A0F2190FA65" modified="2022-10-12" version="1">MNDYMDYIKGIKIYIDDSANKIQDRVAEIVRIDDIKDLNKMQTKKYRNRNNNKQPSLVTSGEVSQAEIELQKLQQQNHQLYEKMKELEEQRTNLKFQLGKYRQMIADKRKLLNKECRDGFQLLTQTRRPRIMSEKKSKSPQLFLTSTSTMSQKKLTQVHILLRDVMKVERQPPFIRSNRRYTFDQFMQLNKTEYFSYIHNHLPKIKQYFLEFSFKRTKQFKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y3C4</accession>
    <name>A0A8S1Y3C4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1510061</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000151">
        <property type="protein sequence ID" value="CAD8208556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y3C4"/>
    <dbReference type="OrthoDB" id="296880at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039918">
        <property type="entry name" value="PPP4R4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21467">
        <property type="entry name" value="PROTEIN PHOSPHATASE 4 REGULATORY SUBUNIT 4 PPP4R4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21467:SF0">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 4 REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="641"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="675"/>
            <end position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8208556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="692" mass="82554" checksum="296B1BADF21631BF" modified="2022-10-12" version="1">MDFVNEKYAVIPKSMKSDEEIQKNMAPESNSFIERAKFLLLKGQIFQKIFVSKNIRESLSDPNGALQLLEIIANQLHSLHDETLIKEYINAVSEIFNKIQITNQLKQSYSDICFNIFTKQYQQYEIINSCAEILKYLDDLEIYKEGLYLETDNQMYQILKIIEIYYHKFTPELKKQAHLQIKSSMKHKDERVRKIISEILLSNQQLLKSILREFDWIDKFTTLIYDSSKDVRMSSAKLFLATYELNEEIHKSRLLLLLENIIKQLDSTQFEFCQSISQYSLNQDNLQSLLIEKITEIINHSNENAFRFLESLHKYYSIIKQFNEKYSIFIDELLLNSLQILSEDMRNYDILKCLSNLYSSLSQDMQFKVIQKLFLNFEFNQLIFIPYLYEIIEKATLINKNKIWLQQLQTIYEMFKRNVFQYETKGLWNEISNMIDIMKIISRILFLQKDFLQQCLKMSHMGSKQIRDKIMNFLIEKIIELDSLESRDQIIQNYTGFINHQNYQLRQLSIEFIIKLCQQRSRKFFISNNLINVLQLQYDPVALVRAKIPKLLFQIKLLFWNDEKEILSRIVQIFQNLMNDKKLYIQQISKDFWAELTNIQFSSAQMTIEQNQIMDEREQQEYANLKMIKQQLLLNPKNIKNEVSKSRNQTPTQRLPPLNKRQNNFLQVPKGPQPRKTSAQRVIQSKSPQPFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8Q5</accession>
    <name>A0A8S1Y8Q5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPENT_87.1.T1540027</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDO010000154">
        <property type="protein sequence ID" value="CAD8209277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y8Q5"/>
    <dbReference type="OrthoDB" id="289751at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45977">
        <property type="entry name" value="TARGET OF ERK KINASE MPK-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="170"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="244"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8209277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="290" mass="34863" checksum="8555BB6DD0F0551D" modified="2022-10-12" version="1">MNSDYREVNQNEEQDQQMYLETPIYKEAVCHFRTSQYYFILFNCLKIGCGFSLLYMNHEQNYMNYWILAIMFHAFIMIIIHKNHLKYLTMNKRILAFKLDQIVAAPIVEQIIEQRKLNILSRQIKLEIVKLTIFGKWCCRIIAIIYQLIFGYGVYLFCIYYSIKSQDLMYQYYFLCTILVISLYQYIDLYLILIFAILGSPFLIILSCYYCANLFSKQQQRNEIRTRLQPIKYKPNQIEGEQECCICRCPYELNEEVVVLKCSKLHHYHETCIMSWININNTCPFCRKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YIA1</accession>
    <name>A0A8S1YIA1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1740052</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000174">
        <property type="protein sequence ID" value="CAD8213171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YIA1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="133" mass="15802" checksum="6660F97691EA25D1" modified="2022-10-12" version="1">MYQQSLVSTIKPLTQTNLNIQSQYEFIEPIQDPVLQVVVERNTIHNYFQLDLELLLCYLKLLKIINLVCQIERCNDVFVNRKCFAINSIYSKSLSYIRFMECQFPPQIYQDSQTSTPKSHCNRCYVIFRLNSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YKJ2</accession>
    <name>A0A8S1YKJ2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA-directed RNA polymerase subunit beta</fullName>
            <ecNumber evidence="13">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PPENT_87.1.T1640037</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="13">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:21249"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="13"/>
    <dbReference type="EMBL" id="CAJJDO010000164">
        <property type="protein sequence ID" value="CAD8211554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YKJ2"/>
    <dbReference type="OrthoDB" id="10248617at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.270.10:FF:000011">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1100.10:FF:000008">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1100.10:FF:000016">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1800.10:FF:000004">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009674">
        <property type="entry name" value="Rpa2_dom_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06883">
        <property type="entry name" value="RNA_pol_Rpa2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RNA polymerase beta subunit protrusion" evidence="17">
        <location>
            <begin position="13"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="16">
        <location>
            <begin position="181"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="18">
        <location>
            <begin position="438"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase I subunit RPA2" evidence="19">
        <location>
            <begin position="541"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="14">
        <location>
            <begin position="647"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="15">
        <location>
            <begin position="1010"/>
            <end position="1111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF06883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAD8211554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1115" mass="127436" checksum="53E2C1367CD4E928" modified="2022-10-12" version="1">MEIQQGDQLRVAAKHHIESFNYLYTHGLVEICRHLSPVEICVPGNRDIRLPFKNMKIWIEELQIGMPTQQGMLGDNRVLPTQCRQMKKSYTAPMLATICREIDDNPKETVRLSLGEIPILVKSQKCHLNGLSAEELVQQQEDCNEFGGYFIINGNEKIIRMLIIQKRNYPIAFKRPGYKNKGFNFSPYAVQMKCVRSDQYARTITLHYLMDGNVYLRIILKKKELQMPVIILLRALTDMNDYQIYQQIVRSQAEKSDISDRVEVMIADAKSRGINSQKDALEYIGRLLRVELNIFNPETTDLQVGRIFLREHICVHLENDQDKASILLLCIEKLYALFIDEINPDNLDSLVNQEVLLSGHLYTAYLREKLEDLLVGVREKILKDATKEQSKLKDLAYFKRCFDLQTTVGEKLENFLATGNLRSQTGLDLMEASGFTIIADKLNNMRFLSHFRSVHRGSYFAQMKTTTVRKLLPDVWGFVCPVHTPDGGPCGLLNHITMSCVPLTHPDEQDIEELLFPLGVQSQRLIYSKNDLPVVLNGKLIGYLNSQLLPTFEHTLRVYKSNGTIKETTEVACLPRQDNKSPLFPGVYINTTVARLMRPVINLKLNTIEWISPLEQINMSIACTQADIRTDTQYQEIDPAYILSILASNVPFLNYNQSPRNMYQCQMAKQTMGSPCHNYPYRFDNKMYRILFPQKPVVRCFGYDDYQFADYASGTNAIVAVISYTGYDMEDAMILCKSSYERGFGHGIIYKSTEYSLNDDKEVKYKLLSQIPIQLYNQIKSQIPVSLLPYGLPEQSQVITKGTAVLALYDSQLQQVKVHHSKDSETSRIEQVAVVNKSENDVSVMIKYRINRNPIIGDKFSSRHGQKGVMSLLWPQIDMPFSESGMVPDIIINPHAFPSRMTIGMLIESMAAKANVLQGEMFETHPFQNYENDNVVDYFGKQLIKHGYNYYGNETLYSGIYGTPLKVDIYFGVVYYQRLRHMVSDKSQARSMGPVDVLTQQPIKGRKKGGGIRLGEMERDALIAHGVAYCINDRLLKCSDYSETYICDNCGNMLATYQFVQLLSLENIQSVQSSHPYCVRCKKSKCSKVAIPFVLRYLVNELAAMNVKLEFSVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YML3</accession>
    <name>A0A8S1YML3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T2870001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000287">
        <property type="protein sequence ID" value="CAD8214993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YML3"/>
    <dbReference type="OrthoDB" id="311665at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8214993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="211" mass="25848" checksum="DBBEB14E3C8AB46B" modified="2022-10-12" version="1">MSFYMKGKQYCQFMDQLNQAKVLQPKKFKSLFGNYMIAIKKLGIMYQYLFIFHYTLQKTVFQALEESLKQDDYGFDDLQLKECKEILQKKEFRFIFIMDSYNEMKLENIQKNLYINNKLKQNWSDPLVIFTTRIIMPTGLRLKIIRDLKQSSIFSLNRIKNKNINILRYLRFKVLKCQFMICMNALDFKKFEQRWERCIHHYRNLMNRVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YMT6</accession>
    <name>A0A8S1YMT6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1680024</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000168">
        <property type="protein sequence ID" value="CAD8212414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YMT6"/>
    <dbReference type="OrthoDB" id="425490at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14811">
        <property type="entry name" value="bZIP_u2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="3">
        <location>
            <begin position="197"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="82"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="180"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="222"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="111"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="180"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8212414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="470" mass="55430" checksum="AE6BA34295AC4AE2" modified="2022-10-12" version="1">MCDPIISEQYYFNDSCDVNVNQIDFSQGTDPFFKNQQQQQSEFPKIQIFEDANNGNNNFGVNQIQIEEIKMDRLKIINDSSVEAQPKSGSEGDYSRDASQNHQNSRRSKKYTKEEQKNLKDSLQSQIIMNLGYTKSTYKNKIQNLVKKETNSHLMVTKKIRQSKSQQKLERKNEQQLLLHTDQSSSENPDSFNDGITDPNRLKQVRNRESARNSRARKKIYFELLETRVQELQDENDKLREQCNSLLKSIENYNKQQDKYSQFLEQQQQLFDRLENCINQGKDVTEIEILLDALKYRTSSNKQERIDAAKSYLYQILNVCLPLQTKYLFSILDDKDFFSQNSRNYTDYLRDVFKKIVITKTNDFGNNEKIKQKLGQSKQNMYDSFKKIKKEIKFIQSEAGKVDLLWEQLKEKLQPQILAQCLLILHKNQFRAEFQASTIFKRVNDKKDQGGMLIEQQQQLLQRKQVKKCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S2P7</accession>
    <name>A0A8S1S2P7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0020511</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S2P7"/>
    <dbReference type="OrthoDB" id="422206at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049680">
        <property type="entry name" value="FLVCR1-2_SLC49-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10924">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10924:SF6">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 49 MEMBER A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="34"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="126"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="257"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="325"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="384"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="416"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8133630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="459" mass="51229" checksum="62D3125B91F7B57F" modified="2022-10-12" version="1">MDQKSNEMEMAMVHLAKPEINDQTEIYKVYKRRYIMASFFAFSTICSSMMFSTCNPIASVLAAVYEIPTFVVSLAANGFLLMHPLLTIVQTFIVNQTDISVSLKIGCALTLLGALIRTATVITERFYTIILGSLVAGMGRPFIINIQANVAKEWFRPEDKTTVMIAFSFIITCSSVFGVIVPGQIFKGYKYETDPDDGKWLTGKLMVIELEIFSAILIPAILFFRPSPPTPPGPINKSRDEFTYLQSLKITAKNKNFILLFICYSLLMGGFHALAVVISYLFNPFGFTPSQTSFIQSSPIICGFVSSVMYSILIRKYHLNHKKIVLFNLIPVLVSLGLSYFALMTESLPLVLLCYSVLGFFVIPCIPLQLELACKVLHPINQTIAVGFLLAGVHIWSFVFGEILSVITHDQNKTQAFYGCLLLFLSFLAAAICFSRVKLPKEDEEEETTQVNQSTQEGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S5H0</accession>
    <name>A0A8S1S5H0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0030574</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="306881at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035950107" evidence="2">
        <location>
            <begin position="18"/>
            <end position="3007"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2579"/>
            <end position="2600"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2673"/>
            <end position="2694"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2706"/>
            <end position="2727"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2747"/>
            <end position="2774"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2812"/>
            <end position="2838"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2858"/>
            <end position="2876"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2888"/>
            <end position="2909"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8134925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="3007" mass="350429" checksum="A8E11876FD9B5683" modified="2022-10-12" version="1">MLQRILIGLLLIQDIQLETIVECYYEYDDSILEINETRPQKYTFTLETNSPNVNAKGFGFWSKYIHNRGFTPQYQGPMPFYDPYCQSQDCQFQGILLMTLLDSYSNLFAALILNMKDDPISLTHDFYLFPIGPNQLQFFQMSFDASQYENVWIYTSFVYSMDDQTIRLFTNIGSQLQTLIYYIISDKITIELGGSLDLSQNDVYGMKPYDFKGYISPIQEYDSFQLMDDFFDNLFSKCFFQEQQTKAEISEFKYFEPQIFEGTDIYFNYHETIINNRYVLKCWIKQDYQEAYDFYKTQYGEEPMQLIQAIFRIDSPFGINKNFVGDAVARFFYTVNFQNNNQTNIHFQTEFYKIPILTPKYQDYSLREFDTLTINGHNLYDLTQKWHYFILEYGRTPSQNGGKLQLRFYFQDEEPQIYNLGTYNYNSQFTGYFITLNLQFYEPFRISKKKLEKIIFITGFQGDNEDKQDCYKSCKYCNGPLNYHCTSCYEEYNYFLTQFNQCECKYLHTYNENTNKCDKIDDQHGLTIYQESVSKFCQFGYFQVLYEYEYFCIQCPQSDIVNFFCGDCYFQFSTWYLKPVCTFDLIQEKNSYAYLKHFRLNSQIDVYYLNSELELQILEGASDFCEDTLHGCQISNNYHLNQQIKLRCKNNYFFNLNSVCKLCDPYCINCQSEHVCLECIESHYFNIRTIRCELCSPQCLTCKTDLNSELGEKCLSCVQSYASTHQGQCQKCGINCQYCKQDYNQQNQQYFLRCLKCFDDKIMAIRFNGIDCLIIEKSHCQYVMIISKSQSIKYNSYSYNFQPSNDLSDELAVCALCDPNYEYIVSTKKCEKLVEEVTCLIGIFHEFPGDLINTGFQRTCLVSKYTYGALSFTGEKNCQNYVSNCNFCFIENTYSPNYCLECFDGYYINRLGGQCLRCPESLNCETCFQQSVGYDDQWKSKTAILNQFLKMRDDKRFFFASHDFSQNPDDYETICISCIEGYKLKNGQCIKYCDSTCESCIFKNDQYFCQSCGKNKYHNLLSVIQYQCSDCPSYCELCRERADEEIFKLNSLFIKTNQNQKFTHQCLIPFLNNENIYYDQNFGQFISCKDFLQCENVLNIELNLFCSYEDYQTQLSQIIDQNLQKEFQMNNIVFHSLIQSNIESNSFSFYETNDIFETLNKLNVRRIKIILKSQQQQTCSIDQFGYISQKFSKYVFSAIDIQLNLISEIEEPLILEIVDDLSFVDFSFVRMENIEISIKQNTNSKIINIYGFQSITLQLEKVIITSYLQIKSTLFNFQCNQLNSLLFNQVHLENIQIDNENQRSIFSFEYVKYQNQISIENFQISNSNITFTNIFQIISLGVYQIFLNNLRINSEFVNCSLLVTSSEIYVFTISNMFITGIMKQTYPFISFQNVVIAEIDNFSIMFMKLIDSTLLQLEKQTRITQFVISTCQTQGQVKFIQNDIPKQSISTHLQYSMMYLIIEDLNSSYQTQILDLQSYSSTTSGLEIYNIMLQNNNLNEFQQENQYLFNIQLQNVKIIDCDIIRGNGLSEFQFSNVLFLSFINLKITQSKITVLHQYFECSSMQQFKNQHPNLIKVMDVINVIFDTLIIERINILNSGLLLFQKQSQTLQSSEDSIFFQNLLLKDNIIITSLNDAYASLISIMSNQRIVVNIIQATVSGNQLHNYEQNLQMPSAVSLLIDCPSGSINLQNNYFYNNFATNTPDSVINIVAKQIKFDGIQFINNSIYNISSLQNNIVYGFSNDQVIFYSNLQSIFQLQNFVGNAYFSADSVIGSNTLIKNSQGKNGVGLYIKAKITKLNDIQFLNLKTFFYFNEENGACIFLEIPSSESQVLIQNIIAYNITSKDYGGLIYIKSDYDNLNLTLSNLTISQCISYKGTVLHASFLNNTLSNRITIKQLYITNQQNAFQNYIKQIVENQIILNKLNERVAVYLENAIVYLSDIIIKNLFGESILLSLYQQNAILNSIKVLNGTILRQKLIQMQPKTNQNTFILIQALSVINIQEFTKPQTQCNVSYVEKKKNYKNMQCQVINQKPLNSLIVDIQQDYSKEIECLHFLFFSSAQIDQQAIIMINLNTESHIIELSEISLINNNFSNSLNGLIFIQRSEIKQDDLQISVKKLSIKNNQCGKVGCLYINSIVFDENKIISTINNRLLAQDYEKIVQEIRHDINIANYVCIGNLADYGTCLFSNHTNIQLRNSLFYNNTANKIGGTIYFIGKQSKLFQIDCQISFNYAQVAGAIYFDDSLAQDMNQSKTIFTDNRAKQYGNDNVQAPVHLSITLNDNKKFFQTFKVDESINYLKEQVNSLNNNHIIFLPSGTPIGLYKKYNSEMQILEQAELKFRILALDNQFTQIKNLKNTQCNIGTQIYNLTSKTYLEVSKSSYISQNSVIFNEKTNDYNLDDMIIYFDNNNTGKSYLQLIITCNSIKIPQFDETKQIIQQFHNNYQLIININTFKCRVGEIKSLSDQSCYECNFNLDQYSLTLNSNKCNIRDEQTTVSVTSFQLNLKQGFWRPYFDNNYIENCDNFNENCVGGWKYGMDSCFLGHYGALCELCDIENIRGDGSFSTAQEYSCGSCDDIRFNIVQIIAFSIWTLFTIVLSVKGAITTENSFLESPFLIKILTNYLQIISSLTSFKLNLPVNFFYFLNGVGNPIQRVSYSMDCQLIQMTSLDIHYSRLIWQLLLPCIYFLMLAIIYAIFIFMKYIKYKSPIIMTAPTLIGSFIAMVSTRTIANVPWIQAYVAYRFDTVIHNRWVLILCLPMLSIFGVIIPFGLLLCLVYNKDKLQYKRGKSVFGYLYYEYKPQTYFWEIIKIVTKELIIIFLIFYEDSIILKGSLIFFLLLCYWSLNLKFIPFRTSRLNLLDYQSSIVCGVSMILGICLNMDQQQQFQNISTVLFFSLIIINFLMLIKMLFYILNAYSREMVTSLDQIKLKILSVLPKRFKSNNSICQKILKTQAETNQRVKANILKLKAALKKHQIQSQKQKVQIINPIFQKENLLILLDSKNKSKQNKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S612</accession>
    <name>A0A8S1S612_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0040369</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S612"/>
    <dbReference type="OrthoDB" id="1517790at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042655">
        <property type="entry name" value="LRC72"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46759">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46759:SF1">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="271"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="211"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8135706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="432" mass="51074" checksum="1D31B634BB179482" modified="2022-10-12" version="1">MDQVFNHLIKRATPQPIVKVGTPWQCIPIKNDKQARDTTEVHMSNQGFNELAQFDKFMNLEVVWLNENQLTSVKGIEQNFRIKHLYLQKNKISTLEGLQNLKHLETLSLFNNELRDLEKNLLILKEFPGLTTLDLFNNPVAEEPYYRNRVISALPQLQLLDRSIITVQEKIKVEAWYKDFKAEVKIKKKGQKKQKLLPSQIFSVGEKILYKEVDEIKAKREQEKADEIERERTRIKLKEKTFDPNKFPTNELKEQLRQRLHDNPMSLVNEWEKGKLKKIFKSYDKEKKGSIQQDKLQGLFGDLMNDIANIGKVPAVNFEKFQEIMSEDPLPWDSFCFQLNQVDFQRAPEEMLQKKIDEKYKEFNRRLNAGKKAEAKEFMIEAVRLEAARDKDEHKLPEIIPDDNTKIRSDCFDFSRYKYKQNFTDTAKYLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S722</accession>
    <name>A0A8S1S722_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">SPX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0030539</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SYG1 (TC 2.A.94) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S722"/>
    <dbReference type="OrthoDB" id="299069at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000822">
        <property type="term" value="F:inositol hexakisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016036">
        <property type="term" value="P:cellular response to phosphate starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004342">
        <property type="entry name" value="EXS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783:SF103">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 53 MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783">
        <property type="entry name" value="XENOTROPIC AND POLYTROPIC RETROVIRUS RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03124">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51380">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="306"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="360"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="387"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="434"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="481"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="508"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="538"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="579"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="612"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="9">
        <location>
            <begin position="1"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="EXS" evidence="8">
        <location>
            <begin position="474"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="668"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="678"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8134859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="803" mass="95570" checksum="0EE653ECFFC90D43" modified="2022-10-12" version="1">MKFAVFLQNHIIPEWALMYLNYKAIKAYLSTSGALKDFLLYAKKTKSPQEYKEVKKNVMEKTYIISKISVDQKEFNKKFIQELSKIKAFVNAKYNDLKGKTYKIQLQILSMNRYQRQLERSKKRSVIRSNSQVSEELTEQLTNQQIKIRESDFNEKSRKLKELCFQLYNEGVNFEKYIKINQEAIRKIMKKQNKKSIKLNQDQINIDECKKLISEINFDVRQNKVKQLLFQVEKYLLKHFYQTKQKLCKEQLRKYQFQNDKNNKAWFQFGLFTGFSFMLMSFIIFLATQKQLDILTNTLIYESFPIYRGALLFILYYWSLTIVIHLWNLSKINYKLYFNFNHHFSTINEQLKRVMTLTSIFLLVSLFYLCDVSKLGIIFSNLKGEEYFPLIIWASLLAIVAFPSKFIINGKGRIWLYRNLLQSLNPKLIEQRHFFIISQFTSLIIPFTDLTYTICEYSKGIKNQEENDDNIKNECFFISRYFTLALVLFPYFILIIQIIFLTQKQQNIDLYIIEFIRNILSIALIVFATLSYQESNFFYYWLGMAIVIGILNIVFVFKKWSYLDIKVKRQKRSISYKQILIMIYIPFGIIQPLSISSSIFGCLDSNEQHSLLILYIGIVELIRRMIINYYIVDAEHFKNKQKYQTVGQYEFPFEECCTRIQKQQLLQYDSDDSDNDEKEFQRFNKETNREDQVIPNQISRRTEIGLFIQGKSTFNQFNNSEDPSQTQVNQLKISELIVKDSEKALGDSFINYDINNFKPDELNSLINCQQISENDLQSLHINNKNSKKEINQLYEEFGVKIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SC26</accession>
    <name>A0A8S1SC26_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0050523</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8137140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="310650at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="38"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="209"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="350"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="837"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="930"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="985"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1146"/>
            <end position="1236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8137140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1480" mass="175535" checksum="51306A5BE404F256" modified="2022-10-12" version="1">MNQNNREQTIVKLQGHLKGATITVHEIEELLLSQEHIIWMQQKEIDDLKKRLKQLESLDIQNEQILHFQNINVQLKTQIEFLKSQIQEKDALIAQFTLKPLSGPSTEMLAAQQQLIQQNLVLQNIIGDLQNQILNKDGVISQLKTQESSLDHLIGQLSANIQIKRSQVGSAEKQKDIQNALNELQTYPEALQEVVQRIIQEYKRISFLIDEKEIEINNLRARLQMQQQKANDSIQEIIKRNPSSFEESRANELIQQNATMEDAISDLERRLISQENRKKELQQKLIEIGTDILQLEEQNNKNNQFNVLDEFNKTEFRQSQQQPNLAEMTFQQQTKDQTFQLQCVPTEQQIDELVSQNQQQDDLIVELQRKLVEKQDQQEEIQLEIDKIDVISLMQELQKLNQQCDKLKKDIEEKDNQLKLLEKQNMELSNVSNELKQSIKIKQEQIDKLYQEEQEKLNLQDALQKHIEYQRDIQQKQQQIDELKLQKENVENELKNVVTQMDDKNIKVEKWNQLQNDQYQKAQIADDLVQQILELDELIQNLEVKLEEKKDSQSQFEKELKDINSKLQTIQESIQIAKTQRIQLENEFNQQKLCGEKLMDNIQELLVDIKNNEIKLEKLQLQEKEQQNIIKELQFNIEKLTEEENQLRQYKENCNIQNDQIQILEKNIEENKKNIFNLKVENNLLQQSMDEIEQEHQKLLSKKLNIENEISQQQIITNNLAQHLETLFTDIKSQEILKQEKEQQLKQLKTQIDSIIIEDQVALLQEKLESQREQNALIEEIERLKYRFENIKQQQQNIELLIENQMNLNQELSEDLLKFKLIGNQLESELHSLTAQQEEMKYQIKEKSSQLENLNEQFTKIDEEGQMMEAVLSLKQKELKQIQQKKENFTKELDQITQEIFDLKKEIALRRKYQQQQNKVDLNQNEDDIEDEIQGKLNVLNTKNNDLKDDLQKLVELSPIPQEQKLNDVSAQISQFNDQQAQKILKDRDKRIEILELTIQTLQQQNHEKNSMMPSLVSKITQQQSFFFEDDPILGELSEKFTKESQLLLDLQNKLRKTAERHYDLSVKLQEWAEKENRLANELNTRTQCVEKIEQELQKVFDKQSDLESDQVNILQRIYQLETENQELLESEANTKNKLKEKKDYIQVLYKNLVEMDQKLLQLRNRMALYSQEGRQLAELVGNQENERENQMSNLQTIKEELEQIELEKKEKLLLIQSIKKEIQNDTQEKEKLELEFITLHSKNSQLKAQIGFEEAVYQKLLQEFEIMKKKDQIKYKNLYKDGCTQIEYEQDQFESLINEMQFSKITKEEVLFLKQNCQQKLNLLTQNDGNQQYFNNNQILKKILHLQQLLIKCQENENKILIEHNSNRSSVLGFRVLDENAQNQTFTLEQIDQFQYKTTIVEIHLNDQCLIIESTNNKEQKHLQQQKMARQWILDKHFDSNNDLYPLGILHIVFKVSIYQVINIALTGTKVLQLYQLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SCX3</accession>
    <name>A0A8S1SCX3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0060373</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SCX3"/>
    <dbReference type="OrthoDB" id="296846at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="220"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="519"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="220"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8137946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="754" mass="87716" checksum="2239B04BA4FE232B" modified="2022-10-12" version="1">MQSIRAQRMKKIIQVQGEVGQHKGYTDIKIVGNAHELIRKSKSTAINICDLAIQDVTKKLLQDQFNSIKSYDIARGEEKPGQQFKSMHSMYDLQTLKITKLYKKKEKNELEKQIRNQLAKDPDKQLLLSEIYEQLEDNSDGEVLKKKQKNREESLATQLEEYDESQDQSADLKLWTKRLKEKKKKEVSKQEDSSTLNIIQERNKQRASTEIKKPIQYQQSMKQLPLDRQNSTQNSIPSSNTPRSYKDVMKKAVQKVKQLKSLNEDLSVERSVEQYIQKASAVALSELKEIGELNVDEEKGLTTNFDYTSEGVQRMYNTLVKVQMSQFKSEAEKAKFYKKQTQLIVNKLVYKIKQNDIIINARVVTQDKLRLETHEFHEKINQIKQLTEQLLKEINDLKDPKTQSSLNKSRMSLVNQFMNQQNKQKELDQDIQILRDSKKAYKQKILFNQKTLDQIDQDIHTKKKENKSFSRCLVSFYYQILKNGQDVRATGIAWVISKLISLSEKCHYSSMPNYLDQKSKEYLLQKATMLSDIEQLEKELSESFKQYKQEQSLDSSQAIMQQSLSYNDSLEALSLPSIFPFDSHKLSSIDLQLARQEAAKSCRNNYQLENLGPITVGLSNEDIESLENMITKLNQQNQPVNNGTLIYKETQKRIKQQIKQKGQNDVSQINDQSAVLKEQAIHSYDKCNTQLNKIKLKLQHLEDEQVIRIVKEFDYKNYGKRFNIDPITVISCLVGGARCDREIVKYGIKKIHYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJ56</accession>
    <name>A0A8S1SJ56_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0080370</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJ56"/>
    <dbReference type="OrthoDB" id="307226at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="93"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8139938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="320" mass="39384" checksum="7C608AD17E51A80A" modified="2022-10-12" version="1">MYRNSNYSFQEPKQKEIDFQAVDKLLEYTKQTHQNSQRHIKQPEYDVIEKQRNCRPNSLTQRISLFESNQQYKRERLSFQDIDFCKIVMFLMAAEIERLREQLQDNINQEQFRKNFEGMQSSINQWRLKFEQKEEECDQLREQLMNLKQINKENYEKNASILQLQIEKLKYEIQESKISQKEKQDEIDTLNLQLKQKQSQLDQFDNTIKQLKNEIQNLQNKHDIKYDQFIDELNNEIEILQQNYRKLYNDYQQYKQCQSQMYEQQTQLPYSRIQSQQQYILQENQEINMLNLMIQQLQKENTKLKDDLFKQSRRTETWCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJX4</accession>
    <name>A0A8S1SJX4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0070261</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJX4"/>
    <dbReference type="OrthoDB" id="312082at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000616">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="19"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="308"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="308"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="333"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="362"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8138684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="504" mass="58181" checksum="40FC58A6A0347CCC" modified="2022-10-12" version="1">MTTSSIQQQKELKVFNNQFVVLKKLSSGSFGVVFQGEDKKTGEQIALKIEKEESDEAKSLDRESQILQRLQGIKGIPKLYWSGSEGPYNIMVIQLLGRDLAYYAKHLKRFQLKTLIMLAEQMITILEQCHSRSVIHRDMKPENVLVGRDNNEIYLVDFGISKVYKDANGNHVPFRDGKPFIGTTRYASIGAHKGFELGRGDDLESLGYMLVFLYKGSLPWQNLQNVSDKEKTKVVGKMKMQIMIEELCKDMPNEFSKYFEYVKKLQFKQTPDYEYLKQLIRKCASDNKIELDYKYEWALLERKTSDRNIKKDSTHNDPNKKQSKNNISTQSKLKPEIDSEKISKSKSPAKQTKRQSLIPEMQSQNQSQSSQFDFLRPPDPYAKARAIIQRSQERHKSVSSLAPSQYSQVNTVQSSIAGNYQASHIDMFATESQQQQQETTEKKQDLIQAQNFGFQPLENNDTIAMLIGEDEEGPCFQYQELLERQVQAQFKDQLKNGNRNKRNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SLD4</accession>
    <name>A0A8S1SLD4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0110056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SLD4"/>
    <dbReference type="OrthoDB" id="302623at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12621">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN CHORD -CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12621:SF7">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8142271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="474" mass="55152" checksum="0C9849B98CC44479" modified="2022-10-12" version="1">MNKAINTIEKVDIFGVPICLLSNTNNQRYQSKFGGIITILLSGLSLVYFVYVIALWINNQIAPNISSKQRTIGYAEFQLQESMIELTLEDFTGDVDPFKKENNIITPLLVTFMNTTVQEKPIPLFSNDEKPYRIELSNVNLVLNTLDSVDETQKLQEQHAIVLARCSSQFLIEGSNCADEKTIDEYLSRFHGFLFIKIKLSQLNLITKELEQFKKLFYTSFDAKKPQYSQIMLKQQQSIIDNGILFNSYENFNFLNNYELITQETDKTYVTKVINAMSSFNYNFDSYGCYLFRIDNISIVEEITYPKLGVVLAQIGSIIQLIFMLKHVVLYYNNQLSENELLNEIITMYYPEFKQYSVNFLNLFKFDKQDINNKLQPIENLKQRYQILQKGAREKCRLTNLLYEISRIQFILQDKFGDQILSQSHQMGGKLLNTQIELQSLKESNRLQIKPIDSIDLENQCYAIEPLEILIKQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SSL1</accession>
    <name>A0A8S1SSL1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0120438</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SSL1"/>
    <dbReference type="OrthoDB" id="305802at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8143855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="238" mass="27786" checksum="4342B1F37EBD48D7" modified="2022-10-12" version="1">MGSCQIQRVESESNSFKESSSKCNYSKSSIIINQIVQPLNTENKLELDQIIEDEENLLTVQTRIKKNKRLYNFQEEESHRKISNRDNVESLKLGAKQKRYLYKTSVTHSHFDEIKQNSPALNQSLQAEKDDDAKSVKSILKKNSKLKDSVSGSPETRSVRFARGTIFRTKSQHSLRQSKNKKHNRRDTRQNESMNSFEIKSPNWKRHREQEKVQITTIPVLFSMNQNIGVKTKLFSYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SYV8</accession>
    <name>A0A8S1SYV8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">KIF-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0130238</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KIF-binding protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SYV8"/>
    <dbReference type="OrthoDB" id="409897at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022083">
        <property type="entry name" value="KBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46321:SF1">
        <property type="entry name" value="KIF-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46321">
        <property type="entry name" value="KIF1-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12309">
        <property type="entry name" value="KBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8144456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="519" mass="60684" checksum="C55F15D61CE93F65" modified="2022-10-12" version="1">MLYEFESILFQVENLEKFPSIEEQPFLYKYRARTLLQQLLQKTQDPLQQAIIHYQLGVNFLDCEENGESRKNMGKSLQLFSQIEDIENYLTYIQSIYNHLGFLAVNLDDNETGLAMLAKAERLGEYLIAKGDFEKRAFKIQGGFEKAQEFYTITLFYIAQAYTKLGIKDKAAYYCGQTMLRQFKSKTYELRDLVINCISLSEYYSGIWNFAQAFYLLLVGEAVIPEGKRKKLRATLQMAQGKVIGDMMSYCVDCIKSNQQNDPSLHKLFNKYSITFDSVNVKQLVFQPSKTFDDIKSLFRQSNTLYKKAMSVFVLDGFVTEHIQMLSEISIMYKVIMNLETDPNNIIAYLEKRLDLLLPVFNAINPKAYQNTYEKLLVEIAEINNELYEFIVKFNEIDDFQGGKSKKLTEKMNKACLNTIQYYQKIVEQLKLFEDKQRDSAYYSSITTAYFNIAKAQSKYYSKDNKIKVTYLVNSLNQYKQLVQFINQQNQQNEFQAVLQMCKEMIQILPSKIDRINYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SZ86</accession>
    <name>A0A8S1SZ86_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial pyruvate carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0150268</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Mediates the uptake of pyruvate into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial pyruvate carrier (MPC) (TC 2.A.105) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SZ86"/>
    <dbReference type="OrthoDB" id="869189at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006850">
        <property type="term" value="P:pyruvate import into mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005336">
        <property type="entry name" value="MPC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03650">
        <property type="entry name" value="MPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8146285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="115" mass="13189" checksum="4DBA9BDD375F8695" modified="2022-10-12" version="1">MGPIKKLQAFVCAQTFWPQFMVRWFQAEKGPFTIFFYCPLAKCGISVANIYDIIKKPVDTINPVQQSVIAMSGTLFARWCWILKPRQFMLVFANSVMASTGIIQLWRKFQSGQSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T4B5</accession>
    <name>A0A8S1T4B5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0160012</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8146708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T4B5"/>
    <dbReference type="OrthoDB" id="421226at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="251"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="296"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="358"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="406"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="3">
        <location>
            <begin position="585"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="863"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="863"/>
            <end position="873"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8146708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1086" mass="128062" checksum="DD314DF941C336C3" modified="2022-10-12" version="1">MQFNLETDNNLIISDFRPEECIDFLSMESISDVGIQFPKPQNSKYDVDPPPNSKDLKEKVILSPLSPNFEGLKECEQLILLSPQNLSQRPIQKKSSIQLIKKNQKQSANVSGSFMKYFHTRRFVNRILRNKHMFHKLQPIHLEIINDASSHYDNEIFGQKSHTFKPLGLMKTLHLSISPSLTKKIIAKYKQISNLILKKFQQVLIQIPVIQPENKGRIIWDVVLSITRLYFIILIPIDMVFEERLLYSEKLIIITTLQTFLLIFDMFINFNTAYYQFGQIVSERSKIIQHNFSKGYGLDAFSVIFLICLLFVNWDQIGEYEPFVFIGLLSFTVQYQNITKLTRIFEEVLNLNKIQAGFLELAKLITLLLYILHISACIWIGCGRISSSVNNGNSWMIKEDILYEQWIVQYLRSFYFCTVTMFTIGYGDLTPQQYVTCIIFIMIFSIQLPYSVNTVGAIIDEISKYSEQKLQKLRIINTYMAKKKITYELQVKIRQYLAYYWECQNTQISQEVKGILEQLSENLRDSLMLEANSIILNSCDMFSKFLSPGFTKALVNKIRYLDVQPENIVDFSDQPDSHQNYYLAFIEIGTIQVIQDQNNCKNDSYIKQLSSGQVFGLYEFITGTYCNEFYKSKGFTKLLILPRNQFLKILREFPEDKEKYCQIRDDLLYNNGEQFLRDIGVQCYICNSRDHISKFCPMVHYCADKEKIIKSHTYIIKQPRQKQRRKVRSIQFNGKIDQKIIMEIAQYFKEDFWKQCQYYEVNDIDQEKQQSPQYSIASDEVKDYIKQPPQLQVAKTSQIIQNKQIIKQQRIGQLNEIQQSRRAARQNTISPLMKFSTNQQIADQYAQNELKVLPRAHSSLVENVSQQESQITPEKQEEEDGTAQKSNQQIISIKGEQIQTKKRCMPQPQQSQFIKRIKNLKLEIEKDPQKKMSLLTVTNMELQENWQSLEQRIIQLKQFQEQEISVMQLQNLSNLIQHQLNDKFMKLENFDLQKDFQYYQPHNNFTQLQVSNCYNKHLIENLSRFIKYLFYPFVYIHKYTKLDAEEDYKLERQRKTMSFSKKKATIVIKFRDKAKQIINSRRIVPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T507</accession>
    <name>A0A8S1T507_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0160006</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8146696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T507"/>
    <dbReference type="OrthoDB" id="10446564at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8146696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="116" mass="13659" checksum="9D855924E7A23469" modified="2022-10-12" version="1">MANQKSPFVHTMIKCQRGHNYEDLIYYNDFGKRFRVSYLRDVPQKYIDDYYNCDLVNIPSKFQLSFDLLEEKIDIVKKTPYSSISNNGSHNSKELIKQLGLQNLTPTKFDKKNLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T689</accession>
    <name>A0A8S1T689_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0160303</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T689"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8147288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="57" mass="6407" checksum="BAED6F49E785C018" modified="2022-10-12" version="1">MEECLNFVLSVFIKAAISGKVIRVFAVIGMLGKANILEVFNQQEKNGEMDCKQIWVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T7D5</accession>
    <name>A0A8S1T7D5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0170089</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T7D5"/>
    <dbReference type="OrthoDB" id="1904536at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050576">
        <property type="entry name" value="Cilia_flagella_integrity"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973:SF35">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT SDS22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08182">
        <property type="entry name" value="Pedibin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="278"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8147688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="481" mass="56738" checksum="6473E8729A849D59" modified="2022-10-12" version="1">MNKTLERNENGTVMMNLRNLKIICDRKDLYQIPENNETLYLHYLGFDKIENLEPYKNLIALWLNNNAIGKIENLDELTQLVSLYLNHNLIQKIENLSFLSNLCILNLSHNSIKKIENLQALNKLQNLNISNNHLTGYESLEGLLECLSITNLDLSNNFISYQQSIVELFGKTNLACLYLKANSFARECSDYRKTMIVTIKNLKYLDDKPVTEGERKISEAWQIGGKAAEQQERLAQIEKRKEINYQNYLQTLERQQRGEMRSNLLIQKENVLREIDLLQMLLIDGDDVQNQIEQKEKELQNIEIELEKYKIDQLSPYKCMLTTKDEFGNIIILNKSEDELKQIKQKYFNEDIQQLNETDLPDLENQNQSESINENLEDNNNINLDQQNNKNEFQEDKKIKILREFKDKKNGWTTDNEQLLQNLLIFHSFDYQIVSKEVKQKMEEQNSQLQYLDPEDIKLIWTYIELTKQKNQKPQNLDGID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TCW7</accession>
    <name>A0A8S1TCW7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0190252</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TCW7"/>
    <dbReference type="OrthoDB" id="10252354at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001335">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF30">
        <property type="entry name" value="MATERNAL EMBRYONIC LEUCINE ZIPPER KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="98"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="382"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="392"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="420"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="437"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8149718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="449" mass="51473" checksum="339D1C577EFEC9FF" modified="2022-10-12" version="1">MDPFYEDYMPTTNNKDIKIYRNGDSNLCCTITIRENTKFHEIRKSLQQKWDTYFNRLRLFNQEGVEITEDDLDYIKNGTVLFASKGEEFDESFQLAEYEQLQDIGEGGFGKVVLGRHKQTGEKVAIKMVKQTLTNAQDVDMIFREARALKSLKHDNIVKIYNAFFLQNLQTVYIMEYLEGGELLQYLQTKGKFEENEARHYFKQLVSAISYCHQKKIIHRDLKLENLLLTSKDSGVIKCIDFGISGFASNDNPENADAGSLRYMAPELLKGMDKAVSPLVDVWSMGIILYGMLFGTLPFTGNTNKEIIAQISEARVMIPTDFNNKLSQNCQDCLQRTLEPDPKKRITSIELLNHPFVTNENTLSTNSNSTNKTKIQTQLQQIDEDDNPVVKQVQSTTNKKQNLQFTKQHPQSSRQLVQTQKKTPQPSPLLQKQSQSVGQTTKQTKFSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TET2</accession>
    <name>A0A8S1TET2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0200240</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TET2"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051834">
        <property type="entry name" value="RING_finger_E3_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931:SF3">
        <property type="entry name" value="RING ZINC FINGER-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931">
        <property type="entry name" value="SI:CH211-59O9.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="181"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="219"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="287"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8150398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="333" mass="39590" checksum="43AEAD4592AC3FCE" modified="2022-10-12" version="1">MIGQIEESSENQSKYITKDRDEIKFKFLKQHFRIQFKESYKYIGFYLLFSFGKYLVCFYSLQQRDQKCDLNPLVVWLTIVGMYEFFNSIRYLAVLHSLQMNSKDIFLLVMADMLTKTSNESMNLSGMQERNRRAQIKTLIKMKLSTLEISQEEWITEPPFTQYEIIHQAEVLNKYTRIMKVYNQFVYYLIFTGGNVAYFQSDSNDCDDNLNNITFVLLLIGYLFSAFLIIGFGLAIISMTLYMPIILFLFIFKSTCKIIKKIQTNNKIKKMRIFHYNNSLEASFIQCNICMFDYVENDKIIQLPCSNMHHFHDQCFQQWVLIKQQCPVCRKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TPD4</accession>
    <name>A0A8S1TPD4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nucleoplasmin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0250016</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8153758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TPD4"/>
    <dbReference type="OrthoDB" id="1902587at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041232">
        <property type="entry name" value="NPL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17800">
        <property type="entry name" value="NPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleoplasmin-like" evidence="2">
        <location>
            <begin position="2"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="136"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="244"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8153758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="340" mass="39665" checksum="EA05455F597072DF" modified="2022-10-12" version="1">MFWGTQVTQGQPYKLTAEEATVIHISNVVLAEGDSKVQVYAKVKGKEFVLANLEKNKLEQAFLDLYFRVDQQVEIGIKGKGSVHLSGYIEPDEEELNEDLDFEDDESEEVEQQKVKKPEPPKQTEQVKQQPKEQQKPQTQQQQKTQVQQQKPQNQQQKAQPEQQQKAKPQATTQKQQQQQPKKADEDWNDNEDGEDEEEELLDQSDVDDEEVEGDLGDEEDDDEEDVDDEEDDDEPEQNKKFIKGNQGQQVKPQQKQQQKPQHQQQQYNKQQHQGNQQFKQGQKPFQQQHKQGQQYHQNKGGNQNQQKNFKKNNNFNNNNNRKNFNKKNNNRGGFKGGRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TQ08</accession>
    <name>A0A8S1TQ08_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0260136</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8154775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TQ08"/>
    <dbReference type="OrthoDB" id="299216at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035855143" evidence="2">
        <location>
            <begin position="17"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="97"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8154775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="352" mass="40523" checksum="E2E00F9737DEF762" modified="2022-10-12" version="1">MKPVLILGLLIFGITAVELESGVPILLKEIKAIKTEHTEFTFLLDLELSQGGKVSEVVSLVGDLLEQMKRDQLNDDLEYAHRTTTLGLDIEWLIIVLRNLTKERQDKNNQLSELNQILVGLQQQLDALTEQLNILNGKEEQLRQTRAQEAASYQARQENNSKVLAALNEIIPKLQVAVFDQDGKSLLQTEQFEIVEKIKRDLGHNHPIAIMVALTTKFDVPTVKRIIDKLEHIRNAVIALMQQEDEQETQSQITFQTVLQEIAELRERFSKDFEVTSQLIKKRTNDKVLLEKRLTIINRDLSLTEQLLIQTREYKEQYDAAYAVRKGKRISEIKTVQQAYDLVENHSKKHKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TR03</accession>
    <name>A0A8S1TR03_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAM50A/XAP5 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0250305</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8154304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TR03"/>
    <dbReference type="OrthoDB" id="295791at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048337">
        <property type="entry name" value="FAM50A/XAP5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007005">
        <property type="entry name" value="XAP5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12722:SF0">
        <property type="entry name" value="PROTEIN FAM50A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12722">
        <property type="entry name" value="XAP-5 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04921">
        <property type="entry name" value="XAP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAM50A/XAP5 C-terminal" evidence="2">
        <location>
            <begin position="319"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="230"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8154304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="706" mass="83399" checksum="DC474DF2C1035B94" modified="2022-10-12" version="1">MLSLNAILNDFDELLQKQQQQDNSMEECKTIDRWARYTQSSKRSLMTRRFSQIEDNKQQTENIKVQSQLFTQEDLNEVKARIRFEQKTKEIEKNAKLKPSHKALEIVNIQQIKAEVRQQLCTKKKKGSPVRVVKNTLQKENKISKYKCQQCIQLLSMGLPTKHYIDINEIEKLKKDQEMMSLKRAPTNIKERFGQSNKDFLEDEFKQKTVGLVTREEFKRKRENIDNLVMQDMKQKQEEETKKKMELKQKRKVEYQKKTTLLSFDLDEEGSPQKSYGKNEHVDTTYLPDMNRERKIEEMTRQLTEQYQKEVESQKDSLIDIQFQYWDASTCSKSLRIKKKMTILEFLEMARREIIRDFGFLTEFSPEDLIIVANGMILPHKLSFYDLIAHKVKNRSGTLIFQFDRRKQKDKNMKQKLRNPPHVKQQRSLDMKKLNIFIHVRNGRMQKSTNTYDILQQLKQQNININMLNTKNSSKQNKSNCINNPKVSIGMFSNQNSIISQLLSTKAKSKFQQHKVSKTTCEFQTFRAETPNSPNKTTPFKTKYHFNTLSQLSKLETNNVSQNIQQAKSKNQDTSDDLGDVSIRLRSPRDDQINKILKNLRFIGEQMTHKKLTQIKSSLLSQWQNQLNDNCDNLMKILSSELNSSSNFYQPIMNNDNQLQKQLAEEKKNRLLVEEQTSKIIQNQEQQIKQYIETIKLLEQKLLNTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TSL5</accession>
    <name>A0A8S1TSL5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0260274</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8155023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TSL5"/>
    <dbReference type="OrthoDB" id="285538at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8155023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="178" mass="20943" checksum="EBD0FFA60C12F7FC" modified="2022-10-12" version="1">MASKEKHLVYKEPSFQFFVKKGLIKQNNKGQVFPIFTYRINTQSSPSKNIELSVIPSKIIKRQNIHQLNPSPFVINNIPKRNTIYQSYIDNIIKRKVKDKPEIRKKYSNTIDEIKGRQIETVKDCFLPKLNCLDRQPQKLKYRHSLPKQDIYTEPNPEPNISDRLQPWDSSSKSFALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TUL2</accession>
    <name>A0A8S1TUL2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0300192</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000030">
        <property type="protein sequence ID" value="CAD8157491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TUL2"/>
    <dbReference type="OrthoDB" id="287423at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8157491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="204" mass="23820" checksum="942EA429029DC0D7" modified="2022-10-12" version="1">MQKITGSGVMIFIKHKEETLVLLLQNIRRKQLVLMEPGGIQETGLSSMSNALKELYEETFAGLSKADLDEEHKVLVNFEYLCYTVYIKIDDLKFFQEVMGMNQKILKMSNNRSLHSYNECKGFNLINLKDLANVNYEYYEQDNNKIPLHQRTKGAFKQLQDKKIIEKDLSIQLPLFQCSLDKIRIKNPKSPLHVLNNQYTYVIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TVB9</accession>
    <name>A0A8S1TVB9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">MIR domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T0280165</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8156170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="76898at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013662">
        <property type="entry name" value="RIH_assoc-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000699">
        <property type="entry name" value="RIH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015925">
        <property type="entry name" value="Ryanodine_IP3_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45816">
        <property type="entry name" value="MIR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45816:SF4">
        <property type="entry name" value="RYR_IP3R HOMOLOGY ASSOCIATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08454">
        <property type="entry name" value="RIH_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01365">
        <property type="entry name" value="RYDR_ITPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="2299"/>
            <end position="2321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="2407"/>
            <end position="2430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="2450"/>
            <end position="2470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="2491"/>
            <end position="2510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="2522"/>
            <end position="2544"/>
        </location>
    </feature>
    <feature type="domain" description="RIH" evidence="12">
        <location>
            <begin position="450"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="domain" description="RIH" evidence="12">
        <location>
            <begin position="1123"/>
            <end position="1302"/>
        </location>
    </feature>
    <feature type="domain" description="RyR/IP3R Homology associated" evidence="13">
        <location>
            <begin position="1925"/>
            <end position="2024"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="11">
        <location>
            <begin position="2418"/>
            <end position="2550"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="2643"/>
            <end position="2677"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8156170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2704" mass="322646" checksum="705EFBE92983BB59" modified="2022-10-12" version="1">MIKQTLKYGQIVRIHLNDKDENGIIVAKGFLDPNAYYMQIDDLRKANNFRDSLFTILPRGTFELHDEYLKEKKNQTVDALKSRVQTEASQYYTIIQSRMHEEILLGAEVVFKHVDSQRYLFGSYDCSEVAMDAFKLKLSPQLSSHTIFKLAAYQTYQKEGQIIQIDEPLIIVHEKTQCKLDYERCRIFLDLQMFDNKINGKASQTESFNKTFKKPINTRNEAVVSLNSDRQWKFQLHEQNGKIVPNDLCFFIFSQDGSYLTSDGRRLILQEYKDTEYIPLDCVWELQLYENKYVIRSQLSGSVIDSSAQLSQYEMLDNQHLQIEPAKLGNNEVSFNMFVKIKFDNKELQKANLLRCKTKKLTITQSEIEQYQKQLGQEDVDYVQYNVDFLQRNSVHGFQIKRLNDDLKKDLLLITSSHEILKQFIDSLQLEDSVLIESIINEKILTQVLKVIKKLIAYLLNCEYINIDKCENLPIHSRQLFMKDLKLVEIIIQIIFYLWEKPKTMSLFLNKNYEKDKLRQICIYSYVLIEKMARNNSSVKLYISQWLKFFLIQAIEIEDEKIQISLAELLNDNYYSINKFIEEATFKGLVSAISSKKKVHEKHLRIFSSIIICDGKPIIKNQIELLNKFFKPQSSEKDNYLFKFLFKQDGKKIKVQNLNKTWLTLKEFYDQSQKQDDLQTWNYFLAYINLLSDICQNRNQQGTNYVNEFYQLSVLCSILEDPETEQVNAIDPFLKLIHSAFIDFSQFAPIRRIARVREWAIIEQKKDIIRTKTPISDDQKRVLSFISHYITKNFNQKEKEKQYFKTLYTVLYVLKTTIRLGYQQKIDDLKNLLEPLFNIITFKQNQSEQKDQLQDRSLQNKISQNHDQQSHKKRFIEINTENMIILKCKQISCEILDIIIDMETNVRVSLSTIKLKEIFEEEDKNINNLILKSTKQLKESEIQKQMKNKIRRLSVIEVHKNKVEIWQNFFSNLIQNRDLFLKFSTDIISKFAELSKYDNEILQTYSIKLLKRIYGYRRELIENFDKTIIVNKGFTHKLSVDSRKTRTKLLHFNDFNILNLNLKNQSEYNFNLWVDKKNIKEGGVIQDLCWLIQCLKQGDEVLYENVKVNYEEFYAENRLQQVYTEKEQNFKLHQSLIKCQDLHENILAFINIATKLVNEVSDQSENENNKVIQKEQQSNSDDKLIMKTLYACFQFLTMLLWNHHENKQDQDFIRIIPNLFQYLKYNLYCIQFLRELFQNNKSLLFNESKITQIINEVVKQCNTLNFNQYYKAQLIDFLRILTIYNNKSIKINQNLIMAALQNRNYEKIMILFDNNDSLFDEIEKLIADYQIQYFKVLKQRNNKDEIKYINLNPKLTYLFNYFQLLVFLISNNNEINLGKCKQMHQHLKLITLYQRTGQCWPLKRYLRAYINRLYYENNKDFIGICQNLIEIDLENILNDLKDIYELRESGCDYQKIKLSNPTRFSYLCSYFYMTLEENLVSLKFIFDENYFLNELEDTLMLNLKISEPVLKQHSILLEFGNILLKIEHLWSGSELITNLIKVLMFQFYYIYRTFDLHTLILRDVLSNPKPFTDVILETTWTHKKYLNGKEQFIQQEQMKQTYQIKQQTILSQKYQELQQKWSEIYSDRLESNNINYQDQLKDILLNEVYDFSNIDNQIKVLKQQNSQDIEYKKTKLKRKYEEQTKINMKLNKRYRNLMEIFLTNKTINEYLEEEFILFCTGLDQIKHPVKSSLQPPITLKEFIQNIIHLNLNHYDTLSQDIQIFFLKALEKIIRKSEYENNPELQNKMVKQQQNFLLECGAAELICKFLRIPNLELRQALTNSLISFGNAFLERGNRKCQNEIYKKLLKDSKNNILINYRQLIRRVSRAVYNNFKFRKNKPQYSFPNIELCDNFDFYDEITQTTTRVNHMENDDVREDNIKEDFINLLNRSFRFLQLLCENNNVQMKNFIRQQIEPDLNPKIGSINFIELATSQLRVFCKAFNSKISEVPIYILDFILEVVQVPCLENQITLCKTTFFEDVCYLVQQLSDKQNQEQRGLLIEQECQGMQALFNIYNKIIIIIMSVLEGNDERIQEDLRKKIDPKFLIEIIKQYLQNHNPQIQSKEDMEKALEEQSLQKYDLKLPKQSDQNKKIQKNNDNIEKILNVLIIKEKIKFQKFDLDENSNYIKEIMKFLETKILRIEIIYENKPQTILFPSRPLFTFLSDETRDIIMYNVNRETQRDKINGLLEYRKQIYRELQYNYELSKREFFPITSQIIQVLRHLSAFFSISVNILMVLFYNMEINNFQVILKAEFYEQLIINVLSFCQLFTTLIYFICYLNYRIPICIDRYRPIGQDLTSDDSDNELNQEESDVSDNEQSNEVQEQIKQFEQNQKKNESKIQQFSHILRYILDSVKKISQQNEDFIQITFYLVFSILGTFYKSYFFSLHLFDLFSRLTLLNNVFQAISHNAKQLIVVSLLGVLFIYVFSFTSFDQYADDIYTEKQPEEHCETLISCMITLVTSGVIGTSMSKWDFVKFCYDTLYFVFFALLFTNIVSGIMIDTFAELRDQRQKIDDDKKNCCFICGVKRAHLEKNLEQFEQHVKDKHFLWNYIYYIYCLKLKETTDYTGLEYAISEMIRKDNISWFPIQFEQEANQDKEIDVRIAQLEGEMREKDDRIAQLELDVKNKLNNINNILTQQLEKEIPSKLENLNYLITEMQSSKRIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TXR6</accession>
    <name>A0A8S1TXR6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0270157</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10035969at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332:SF175">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="26"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="32"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3342"/>
            <end position="3365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3454"/>
            <end position="3475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3487"/>
            <end position="3511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3523"/>
            <end position="3550"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="172"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="351"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="461"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="516"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="613"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="660"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="804"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="867"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="915"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="962"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1014"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1058"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1157"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1203"/>
            <end position="1244"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1252"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1300"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1347"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1394"/>
            <end position="1434"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1435"/>
            <end position="1483"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1484"/>
            <end position="1533"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1541"/>
            <end position="1578"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1579"/>
            <end position="1607"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1629"/>
            <end position="1666"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="2118"/>
            <end position="2155"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="2156"/>
            <end position="2184"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="2206"/>
            <end position="2243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8155536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="3589" mass="411687" checksum="19AC0B277BB090AF" modified="2022-10-12" version="1">MFLIRLFLIIEVFGWRFYPSYYLFEIGFSKSQCAQLHIFMPGLHPYKCVSYALICKGVTEVLVKTEVNGTQHLCHPGFQLWGAGTPTPDEYLVHCGFANGVSLVMEQLPDQNYKYYCKYFADNSHNKCLITAYQQGVFRCQFCKRPFYGNGFGCDQVQIRFDDRFIYPNDRECAGFCSQCNSDKKCIQCMLGFINVGQDTQCSQVCHGHTNCYFQSGYFYGYGACLPGYMQLGSNCYDIAPCRSASPLPIRGYTCESCFQGYIKKVTYGSAYSCYLMDWNCDPSRQSMIFDYQIGNYMYYYTTCTLCDPIYTYSRTQKKCIICDSKYNLIVDDGICKCKPFYGFLEGICTVCADGYCQNCESDDFYQCTSCKPGSNRIVINKECICQPGTYDAENDDQICIVCDKSCQNCFGPLIQECIDCVEESISNRIQIGNVCQCKTGYSEHKVKEQICGKCHAKCLTCFYPADGSENQYCLTCIPGQNRVVSDTFNCDCEENYSDFGGTLEVCAVCHYTCGNCYGVEAKNCLQCSESSYRELTPLGECLCKQSYFDDNTQNRKCQKCHHSCLICANSTEKDACLECPSSRTAQSSGSFFECFCTDSNTFDDGFSLQCQQCDQSCKTCFGPLSANCLTCDTNYRQLELSFCVCPINYYDIGQLECAECHYSCHRCFDNNAESCIMCSFDLNLRVLKGNVCKCVDGYYEEVGIAKCQKCAYKCETCDIQPDKCLSCPLNSMRKLDPIKGCYCSEEYFDKENEITCLKCHFKCKTCKGIEENDCLSCNSIQHRELKNNLCQCQPNYFEMEVQECSTCSPFCYECINNSKNCTSCNDDRYLDGNTCKCKTKFHEQTISTFDFNGMLTCQKCHYSCGTCMGMDEENCISCMDTENRFQVGNTCVCREGYFDTGLPACAKCSYKCKGCQKYFDRCISCQDNSLRLLVSGFNKCQCIQGYYDDGQNNVCQKCHFSCLKCNDIETKCELCSNESNRIYNDQLFSCDCNMGYYDIGVENCQKCHHSCLSCNQGDANSCISCIDLNTTKRVFYNNTCKCLFGYFDDGSSISCQKCDIQCLNCVGQSYQCLSCPQTRKIETNCKCHQGYYDIGLQFCSKCNPKCLTCETTSNNCTSCDPNQFREINKINRNCDCQIGYIEIDGICQKCNSSCKTCSQSLNYCTSCIQFKNLKNNDCICSDGMYESSIDKSCKLCDKTCLTCANTNNYCLTCSIDNYRQLKNGNTCECLQGYYENPNNQNCDQCMSSCLTCSLVYDNCLTCDNNLNLSLVNNRCLCSQSYFFDSVNHSCQQCNITCLECQNYNECTQCRFSTRHLVVDLNQCLCNDGYFETNQQHCQQCHFSCNTCENIYTNCLTCVSQYQRILINNKCLCLDGYYEAGIELCYKCNDLCKTCQSFATFCLSCYDIEQNRYHSGDKCICKPGYYEQNTLICSKCSNECLTCQGSAGYCTSCDTNSKRVDQSIIHKCPCIIGFYQDQNFICQKCDIKCQTCINQSNQCLSCNIQLNSNRKSLSDQCNCKDGYFDDGTQIQCQKCNLQCKTCEIKANNCLICQNSMRINTPTCNCIDGYYEDEQLNCQICASKCNTCLQESSNCLTCQPGRIEKDCKCIDGYFETGQKLCQQCAFQCATCVQDPFNCKTCKGNRLQEPLCTCESGYYDDQLNEDCQKCDSTCLQCNMNGCLSCFANRELNEEMNCIPPPNSIWYDDTPWCSTCQVAVVNSYLSDDLSMIIIHFDFPLNPKSFDSYLEVNKCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNRKSLSDQCNCKDGYFDDGTQIQCQKCNLQCKTCEIKANNCLICQNSMRINTPTCNCIDGYYEDEQLNCQICASKCNTCLQESSNCLTCQPGRIEKDCKCIDGYFETGQKLCQQCAFQCATCVQDPFNCKTCKGNRLQEPLCTCESGYYDDQLNEDCQKCDSTCLQCNMNGCLSCFANRELNEEMNCIPPPNSIWYDDTPWCSTCQVAVVNSYLSDDLSMIIIHFDFPLNPKSFDSYIEVNKCSQLFELESVQNFGQNSVCTINSQNNKELFIYLGENSKMNVGEAIKFKVNSLSHINCETTLKTFIFATLLQPKNPLPPKINYHVPLHKLNPFAENQVYLQSIKNNGNRKLINIDWSCQVESGESSSMLNQFLDQINFLQEQNLLIPKLTLPSDAVLKFRIQYQNFILISSYSEFTIYTHAGDIPQINIDMKPSYFLYETINIGVSVGTLVNSISNDSSRYLIQLIEIDRYPKKSSSSNLNTTIDSNSFEMVYTTIPRYTLSKNSTYTFQVTATNLNSNISNNLYFTFETKMAGLFCQFNNQGIQNIRRDLNLQIQCEDFDSKYERQSDPDLNIEVACKDLSFNTSCKNEQKQIINVNKTDSLQFIQKNTISTFTVQEWTVTAKKFQQISNFAFVIVYLDDDFPQLELQFNKGYLMRKINNYELLNFTYLIPFEKKIHLLDLTITIIYDYQIIEILQPKFISHHFKIFNSIKELNFGEKINLKFAAQYTNNIMPSINNIKLSINQPAKCSKLLINRQSDFALTDFLVATTCEQSNDSPYKYQLRQFLRESDLTDFLNGISDNSLIFYPFQTQNQFSIQAPSSIDSTKIGILVEVLDNGGSITHKFEKISIKPAKINCLSIQFKNLTLQNKITLLFEAMNQKCDELHQQIYLDLIKSQILPEKNANILKFQANKLYQQFLISQEENKTKNRLLYEEIQKKCLDLNSNHLFITQNSTQLEVNITQKIENFKKNSEDFNRIQKYFIKMKKQCEQELQQNSYIWNEQIFQQYLNSQDCLKSLIYYLDDIYSDFSLINIKDETIDQIIEELLRFFDIIAEETQNIILVNEKPLVIKGFEIIWKIKRRTKQLFNQQFNIQQTQEDYLIDFIQFEYAYLQTNPLRFNLNLEELLKTYFNDQTIQISPDNYYQIKLKNFYNRRFQEYENFSSIYDTQFGSYQVCQNNSQLLSEYEIYCVIRTITGKFYQCNLNRVQNNNRIELHCECDKFGEIFLTTSTKLIVNASESPKQIYNLELASKSEDILPLLLSTCSLSLIFLSIFIFQLYKDKKVQQERVDTEQSNLNPPIIKEKNSLIYHGTSKVYIEKFKQIHQTISLFYYKDKNIEFSYRILEVFSQFNLQLTLTILECYLLNNQVLLICVFIILNPLIILLMRIFYKIIEAIYRFKRIAAFISHLLQIILLMSPNLIIYIFYISKISMQSEQYIFAIIYLGNILISQILIEPLTVFGRILIYRLIAKSLKNMDLNPVFHLMHFFVMHSSLEEIFEEFTKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U0W0</accession>
    <name>A0A8S1U0W0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0300247</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000030">
        <property type="protein sequence ID" value="CAD8157593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U0W0"/>
    <dbReference type="OrthoDB" id="302603at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8157593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="79" mass="9486" checksum="03DC493262A87E82" modified="2022-10-12" version="1">MQEELNSSLDSLELELYHKPNKKIKSILTYLRQQKIQFCTDPKKKEDDYYSKNDKESDCKDFGLFKNKFYKNKSITPIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U1C5</accession>
    <name>A0A8S1U1C5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0300141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000030">
        <property type="protein sequence ID" value="CAD8157417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U1C5"/>
    <dbReference type="OrthoDB" id="290449at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8157417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="107" mass="12713" checksum="A9943759421BCD21" modified="2022-10-12" version="1">MGCCAYKGQNRNKEKVFCEPNISDTQNYRQSQDQSLQPKYQEQTMEKVTCEIQIVQKNNLNHLITLKENQYTVYNKDDLNVVDDQFFQDKHQQKPQEVEKANSDDDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U4J0</accession>
    <name>A0A8S1U4J0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tubulin-tyrosine ligase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0300187</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000030">
        <property type="protein sequence ID" value="CAD8157486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U4J0"/>
    <dbReference type="OrthoDB" id="196367at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_125, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069">
        <property type="entry name" value="TUBULIN TYROSINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8157486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="615" mass="73067" checksum="0C5D2B65E18CA58E" modified="2022-10-12" version="1">MKKSFTSHSPSSDHYYIGPREMKLPKFEFEYPPSSYGLVVSSTKNSNKTELISDSSSESLLPPLIYFKIKEKKLQQEQDEKCVLVGNTLHQMPKKIIKPKISYQPDIFPLKALKKKIVKQKFNGIYFEDERYSSDPEFQLNYANLRTYYNSINISNNLYVQPMINSYKAYIGKGNNGQLVQWILKRRWWWSIVDKEKEQVNFLWQQLRNNSYIESIPESNQEILQQQFNNQEQNLIIQDIQYSHLLPNQLSFQLQKMRKKVGKNSKIISYDQALKFSQTLRLFEVNGNSKLHNHIQNNTHLGSKKNLFYNMKRYYEQQGLNVFDFLPQTYHIKNKEDLQNFVQQNHNQSIWIVKPAELTNRGHGIHIFQTINEVQQYLRSIHHHKNGAQKTFIVQKYIENPLLYNQRKFDIRCYILFTSVNGQHKGYWYKDGYIRTSSQEFSLMNLSNKLIHLTNDAVQKYSEDYGRFEKGNKVSFEEFNNFLPIDFYQTIYIQMRNIALDQFKATYGKLDPNRKENSFELFGLDFMIDDKFKVWLIETNTNPCLECSGPLLTKLIPQLIEDLFKLVLDPLYPPPQFYNSKKFIYDSFENKFELIFDSSMLDVPLKFTQNLIDEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U5W3</accession>
    <name>A0A8S1U5W3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0320108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8158709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U5W3"/>
    <dbReference type="OrthoDB" id="302623at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12621">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN CHORD -CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12621:SF7">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="316"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8158709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="474" mass="54666" checksum="08461936DD123094" modified="2022-10-12" version="1">METLPKQIRNLLKQIDLFSIPVPVLTDDAKSKYSSPCSGLISILASVLSLAYFAYVLSEWANGNILPTITSNQQALAYSEQELEDNFIQFKLADPSNQDPFQRNNNIITPLLFIVEDYQIASAPLPLFSDDKNPFTVGLNKGTLIVNNLNQKIDEQSFKPQKNYLLIFIQCLDQYLIDNSNCASKEVIDQYMTEYHGFLSIVMKLEQYNYRQDQIEYITKQAYQALSPTYIQYTQIAIQQKAINLDNGLLLQNQVTYKLINDFYLINQSIDNQYIQNIVSSFSNQDVKLNAIMAYQIRIDNLQISDRIIMPKLSAVLAYIGSIVQVIFMLKYLALFINNQNLKIEMQTEILQIYYPNLKNMIIKRNYLGKIIPINETNQQDTQNEIFIKQYEEHLDIAFKKLRLINIIYELSRIELLLEKHFGLENVSECNQMGSKFQFISNDNKNQSSRVSIVSVGNSGPQPTDDIFKIFNKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U6L9</accession>
    <name>A0A8S1U6L9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0330107</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U6L9"/>
    <dbReference type="OrthoDB" id="292319at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12885">
        <property type="entry name" value="SPRY_RanBP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013144">
        <property type="entry name" value="CRA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044736">
        <property type="entry name" value="Gid1/RanBPM/SPLA_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00757">
        <property type="entry name" value="CRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00668">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="1">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="CTLH" evidence="2">
        <location>
            <begin position="329"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8159387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="493" mass="56897" checksum="734143D45FEBB303" modified="2022-10-12" version="1">MQIEPYINTVLKIQDNSTLTDAKLNRRKHQNIVYENDGLTAKYSPNPETHEYVCFQTEKAITSSFYYFEVKILKKENAKNIISIGLAFDNYQTNKPLGIIGGSIGYYSDGKVILKKQEIDLKLNQYKQDDIIGCGIHKSEVFFTHNGIRSLQTVKIDFKEPLYPTVVCGDVVALRFNLGASPMIFDYQKMLQKEKNEIIQEIDKQDVSPYSLHLIIQEYLWSQGYLNSLKQFERESSLQENENMRLEKKPEEMNYEEEQQCDGDLKRKQSLQMTPMSALQRKLSGLQSPNFQQISSIERKVSGFYLDEQENNNELNQIAEQAFLKDQLVNQERIKIQKLIREGKIGEVIDILLDMMPGFLQKEGIQQTLYAQYFIELLKKDKVQEAIVLGQIHLSQNLQYQVECVDEKLNPIKIKIESILGLICYDDIGVSALKGLISQQQRERVCDYINRSLLIELGYEDESALEICLKQLIQVCGQNQQRGLLGGHSVQFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U743</accession>
    <name>A0A8S1U743_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pectin acetylesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0330213</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U743"/>
    <dbReference type="OrthoDB" id="2015280at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004963">
        <property type="entry name" value="PAE/NOTUM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21562">
        <property type="entry name" value="NOTUM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21562:SF67">
        <property type="entry name" value="PECTIN ACETYLESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03283">
        <property type="entry name" value="PAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8159567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="382" mass="43865" checksum="3DC5420B82F0B165" modified="2022-10-12" version="1">MYLILYCIIHISIAVQTLRQVQDQNALCLDGTRASYYFEQGYGDGADKYLIFYEGGGWIQGFDEAEMLQQAYDRSNTNMGSSKFSAATTQMDGLFNRNQNVNPYFYNWNTIFVNYCDGTGHQGYRAQPLQIKDKLIYIRGELIFKSIFQEHLTKLTKASKILVSGCSAGGLAVFSWIQYIKNHLPSNVLVLAAPDSGIFLDLQPYDGAQAASDRRQKQYHKLANEEVDPLNEQCVKSYPNEKWKCHFAQYLIQFINVPIFFMQSLYDTACIPNILHIYNAWDYTLKRCDYKERSCIEAMRKQIINLINDRKLADPKTGAFGVSCLEHCFTQKSIFYTPNWAIPNGSQWTIANSLGQWVQNLELNNMHVDNVYWPENNGCSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U960</accession>
    <name>A0A8S1U960_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0360300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U960"/>
    <dbReference type="OrthoDB" id="26371at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000068">
        <property type="entry name" value="TBC domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000028">
        <property type="entry name" value="TBC domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.750:FF:000007">
        <property type="entry name" value="TBC1 domain family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF26">
        <property type="entry name" value="LD44506P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="1">
        <location>
            <begin position="73"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8161665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="385" mass="46201" checksum="1E1BA95F26223653" modified="2022-10-12" version="1">MSFLKKLFTKSTKEKETQQVQHNINQIYNTQPFQRKSNPIYNYNCIYRIEQFEIILEQQIIDLEQLKQLTWRGAPQVYRAKVWKLILKYIPPNHMSQTNVIQKKRQDYFQYIKNYYEQIYERDESEEKIIKIISNDVLRTQPDFQLFRDPKIQKLFKRLLFIWSQRHPMSGYVQGINDIASPLIAVFLNEYVRVDYNKFDLPSDFHKLPDDILLDIEADIYWCLCKIIENIQDYFIHNQPGVSLAYEKIKNILNTIDPTILSYFQQQQIDFNHFAFRWVICLLVREFPLYLAIRLMDTYLSEGDNMANLHIYTVSNMILKCGPEIKNKNMGEAVIFLQNLPTKDWTELDIEMLLQEAYVFKEYMTNKQNLNQSMTPTQSNRISNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U9D9</accession>
    <name>A0A8S1U9D9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0350007</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U9D9"/>
    <dbReference type="OrthoDB" id="285825at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8160483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="56" mass="6432" checksum="6CA546F27B382894" modified="2022-10-12" version="1">METLIEKKNCLQPYQELIICIKQAASLSNPNKRPSYTSICQQSFQKLGQCVVKEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U9T6</accession>
    <name>A0A8S1U9T6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0350094</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U9T6"/>
    <dbReference type="OrthoDB" id="299305at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035747787" evidence="2">
        <location>
            <begin position="22"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="231"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8160653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="359" mass="40362" checksum="55C52F14898CCC1F" modified="2022-10-12" version="1">MQKLAILTLVVALAASKFVDTHTTLAQIDANPFGNVVLSAIKAHIQAQTPANEVNMLLNGVAAGLQQDQNDHDHAFELDTTTNNRIVEDLEKEILYHQNQISSNTQLRDDTIEALAVSEEDIRVTIQDIATNEQTYAREEATRNQQHETFVAKVAAIDDVIDAIDEAAKLIQHLSLGASFVQVKSKYETIHKRLTDNTSHSQLLQPVVAALTELATHGVNQKALTKIAQLLSEIRQQLVQEKATKTDVEDRQAAHWAEFSAHLSNEHTRLVERKAQLEVQIQEQKDTIEDAQSWIEFHTLELENSEERLAGQQAWYAVQSEIYETQTAERAAQNEIVDRLQEHISEKLSTTAQFISKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UBF9</accession>
    <name>A0A8S1UBF9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0370240</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8162180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UBF9"/>
    <dbReference type="OrthoDB" id="308392at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="92"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="186"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="437"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="128"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="94"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="186"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="453"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8162180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="540" mass="63776" checksum="DA63A07DBA35A1AE" modified="2022-10-12" version="1">MNRDNPNQYRSPQQIPSSKKPNMENFPPQRNTNEFIKQMKIRVDQPPLSVMTQNSQIKDAQSIFIDISRIEQQSNNSNNSFFDQLKQPILPQCHRSNQPSNCTTQRTNRQEMQRNSKPYLDQTDNMGLLLIQQQMLQLQNENMILKQQLEKQESQRLLFQQQLLQQFQSQQQQQQQPIVINNNITSSEKQQQQSKSISPINNQNEQESPPNQIQRKKQQSQAIQTDKSILDQSIININESYQNESLCNQSKLIIQQQSLIRPLQQQNIESLTFGTQLSQRNLISQQSQQQLQIQTHDSQSQNQSYLQFNSNSHQFQTEKIQKDDKFQNKNDIQRKYSQIQILNSPAQQDQSYSVHNPDIRKQSQPSVKLEFNFEDGIQNQSSKKCLADYFTQRKKAANVERKVSVQVPIQEDDQSWIVNVKQRSKEEQIKLRKEMMEYGRNKRQQNKSQTPINEDKNSDRKEQIKRAKSPLMERLAMGQKAKVEEKEMFALTKKNYENLPEVKGKKNQEEVIKQKQLFMKERQNKLKELDEKIKEKMKKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UFD5</accession>
    <name>A0A8S1UFD5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0380028</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the eukaryotic/archaeal RNase P protein component 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UFD5"/>
    <dbReference type="OrthoDB" id="128536at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007175">
        <property type="entry name" value="Rpr2/Snm1/Rpp21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14742:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14742">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04032">
        <property type="entry name" value="Rpr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8162422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="119" mass="14371" checksum="6BC84B0D01447CBA" modified="2022-10-12" version="1">MVQYVITSEDIHKRKDNDAYFRLNYLYQASLLFQQSNIEYAQFMKEQMQKVAEKVPIRMTPQIKHTHCKKCLCPIVFGEVKFKKICKKLFIVKTCLKCKFQRKIFVKKLKDFELLQKQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UFR8</accession>
    <name>A0A8S1UFR8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0400064</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8163788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UFR8"/>
    <dbReference type="OrthoDB" id="342264at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17744">
        <property type="entry name" value="BRCT_MDC1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16770">
        <property type="entry name" value="RTT107_BRCT_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="501"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="352"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="352"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="385"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8163788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="660" mass="75892" checksum="57B8D216EAB16F2C" modified="2022-10-12" version="1">MIQVLDQNDREIYKYRLLQGENRFGSDPKQCKYLIRNVQGVLFSVALLNNDVYFTHFSNSDQVYKEATLKSDQKVEIPKNFIIEMVPLRNYYFQNFKFKLLNSIETIQQNNQCTLQTTQILEDFSGTMALDDILDIPQQFIPKVSITQQMSTTQVLSSQDPIVTSTLILDEIDQSTLSSLNELISQYSGINSMVKSNHFSTNDAQTQTEIIEVEPQLQKTRTLFSDLFKSKRQTSQISQQIDQHNQQPSFIDQTMIDSQMQNIDQNQQNNQQFLSKKADFKGTMIDDDLNKAFSSQQIKSVDKLEQSQIKRFNSIQSDNVQITQSKQDNNNSKIELNDLFGSLPELPNQQIQQQQTIKKFPQPKSNILNNRYDDSQEDKNKSQDQQEQTPIKVQNQNQKQTSSLWKQGKKQSKDDLKKVALLKKITDEISDETSEKSNQSKQKLRHTNSIELPATKIFQQNDLKINKKLGNKKTLQKKVKSIHLIAFSGFQANEIPQANDLKKLGLEIVNNQLEKFDLLILNNPPKRTFKFLSALMLGAEVVTMDWLTNSIKQGKAIQKYHDYIPYSDEFINSFGLSIEQILQSRDTYFESNKNLVKIFDGKYHVKIDVTPSKAEIEYLIKLGGGEIQQKPGRNVITISDTKVGNSMPSDFILDGCMFQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UHN9</accession>
    <name>A0A8S1UHN9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0400342</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8164340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UHN9"/>
    <dbReference type="OrthoDB" id="294616at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8164340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="612" mass="72067" checksum="254B573A5BDC6E42" modified="2022-10-12" version="1">MDQQNDVVTQGQQQELNEQQDETSTQNEPIEQPIAENPPITETIPMIDPLIDQNKDQPTNQDNKTEQSNDPPASNPIYTYSILAKEFILFSLPYLYFIFAALYRIYQLQKLQAVTLQRQSIRSQLSDEDIPKTNPPSLNIKIIISYWLCLVCTVSMTLGFFGLIMTISPKLYFIWLIPIVAHIISVILLKLEFKVQEQQATWTHRIFWPLQTAFLVVRTTQEYQTLLSSGLIILQLFPSLILLIYALYRPNDRTRIPDDTPKFAKQLMKQLEEFKIFQSENNSNRESMLNRTKAKKENTTISKQLSSAFSDLIQSKNQNEQEQFLREKMPERSVSVSKKVIKRFVNDNEILYSYMIITIIEGHTFQSEKRYPEFLNIERVQIDHFNKYNASSFKIQRIDLQLGEDEFSYVLRRREYLERWLQDQLANPTYITKNLLDFLSVDEEMQQPFLIYQQLISKTKSQIRPKSYQKTQEYELQAIRVNSEENLVALDYSELQFSSNCVQYEYGMYGSNSIEFVINIKYKEKSTEHVYRILKTLSCIRQFSEQLELQSGRSLPPNLKVTKQILNLTTNEKITFVDEFFRELLGNHEYFSSEFFDFIGFDINNRCPKDQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ULR8</accession>
    <name>A0A8S1ULR8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dipeptidyl peptidase 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Cathepsin C</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Cathepsin J</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Dipeptidyl peptidase I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Dipeptidyl transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0420166</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Thiol protease. Has dipeptidylpeptidase activity. Active against a broad range of dipeptide substrates composed of both polar and hydrophobic amino acids. Proline cannot occupy the P1 position and arginine cannot occupy the P2 position of the substrate. Can act as both an exopeptidase and endopeptidase. Activates serine proteases such as elastase, cathepsin G and granzymes A and B.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>chloride</name>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Tetramer of heterotrimers consisting of exclusion domain, heavy- and light chains.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ULR8"/>
    <dbReference type="OrthoDB" id="640249at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.80:FF:000003">
        <property type="entry name" value="Cathepsin C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000257">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014882">
        <property type="entry name" value="CathepsinC_exc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08773">
        <property type="entry name" value="CathepsinC_exc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="10">
        <location>
            <begin position="247"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8165344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="478" mass="55232" checksum="C8BC8C4134F9C74A" modified="2022-10-12" version="1">MLLVFLLVLSVHADLPVHCVRHQIVGKWQLEFTEPQIKGSGPLTCGHNVPDNERTSQIAGQNTFKKAFTHEVILTAKNNVVQKKKLQGKWTMVYDEGFEVDFSHYKCFAFSKYKTNYSGAYSYCGETLIGWYQNTETNERGCYRAYKIEKTHKVSEGANNGHVVQPQFFQYQNSGVNKIWSRIDALNFVEQINRANLQWKAKAYGEIIGLTTKQLNKYAGRRKHSSTILLEKQKKKQQLLEYDLSHLPKEFSWEKYLGKKIYEQKGCGSCYTISTMTMLSARLRIKGLKVDLSPQQSIDCNYYNQGCDGGYPFLVEKYLTELDGMIFEEKEYPYKGQVGTCKAIEKKKKFRVINYRFIGGAYGKSNELNIMEEIYKNGPVVLNFEPSFDFMFYVGGVFHSTIPDWIINGLAKPEWEKVDHSVLCYGWGEENGIKYWLLQNSWGKQWGENGRFRMKRGQDESSIESMAEAADIEIIHDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UNC4</accession>
    <name>A0A8S1UNC4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0440199</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UNC4"/>
    <dbReference type="OrthoDB" id="312779at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8166540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="386" mass="43917" checksum="49ED873AC3DA03AD" modified="2022-10-12" version="1">MDYFPCAIHNGHMLSIICTDKSCNNNILICPLCEHSDHQNHQTLPLKIILQLIESTSNNTNEQSTQSIQDVSFFLDRSYNECLRIVQKFNETLENTSKQLIKKVDTYYLDLKNQLKTYANSKIPIHYLLDSINNQTDFQQYKKNIQQILEQFAFSNGIASRQSIDSAIMNVCKTHIGQLNDQLIDLQNEIDGKVENLGNLLRAQPIASVDLVQTISFQFSSQYKGANIKISGLKLFAEQKASESNGQRFIICEPCIPKNGIYKFGFKVYKYAGWIGIGVCHRELIVNANFKFNYTNIGHGSYLISNNAYSWSHLQKDLNSAHKSFDFGVGDIIIIEVDIPKKKITWSKMKTQLKITMNLDTSQDLYPCANLCSPGDTVEIINKIED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UPC9</accession>
    <name>A0A8S1UPC9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0440050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UPC9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8166254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="86" mass="10361" checksum="77446B2C4BB68457" modified="2022-10-12" version="1">MNYLKNALLLISNSNNKSIFQFDTMNNRDNVIDVKTMQNMFKLNNMLIMCSVIQFIIRFEFSMQLQFRVLLEQKQLFLIFLILFNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UTL9</accession>
    <name>A0A8S1UTL9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0480072</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000048">
        <property type="protein sequence ID" value="CAD8167885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UTL9"/>
    <dbReference type="OrthoDB" id="301906at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="98"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8167885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="364" mass="43389" checksum="41F0D544874CBB7B" modified="2022-10-12" version="1">MLADKTSAYVNQSSVQSKQPSKRVQLDIQYVAQTFEKTQSINNLQDMFPDNQSTELRNSMTNEESEIEKLSQAYADKYYYEMKFEQYQNLLTQQQGTITKLLNEKKDLLARLESAKSNEKEISLLQQLEVKFIESQKEKQQLQMECQQYKYQVEQLLVANNELQQSFQSLHNQFVEYKDKQDKYSIKQQELIAKLTDDIGNQKYLQEIHSQMQNIKQQNNQLSEQLSQQELNFQIKLQEIQEKYLIQTNELTQDCESQIEQKEEELHLLKMEYEKQKSETQKLHNENMQLQNQIQQLTQNYQDDIQAQASLLSEQVAEYKIVIQNLENQLYQMNQEIKQRDRSSSKDSMINSRLISNYISRYKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UUB7</accession>
    <name>A0A8S1UUB7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TFIIS central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0480210</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000048">
        <property type="protein sequence ID" value="CAD8168145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UUB7"/>
    <dbReference type="OrthoDB" id="10259622at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003618">
        <property type="entry name" value="TFIIS_cen_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07500">
        <property type="entry name" value="TFIIS_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51321">
        <property type="entry name" value="TFIIS_CENTRAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TFIIS central" evidence="3">
        <location>
            <begin position="233"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="113"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="220"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8168145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="458" mass="54454" checksum="1280D687488DE722" modified="2022-10-12" version="1">MTDIIEKVQLSKLPEYVQMTEKKGYVMRQQFISKDLMPLLKQCFCGQYVNPDQILVLCPSCEKPYHAECLMKQFDQGNANCDSCREVLSNLQITDRIKDALQTRPFTVVIQSNQQKNHDQQRAIQIEEEEEEEQLDRNERRIDLEKIVKKIKTKDGPIQSKVQIVKQDSNNNGNSRNKNKSHEITIAQPNPPEQQKQQSQPFKNISTQAVEKMKSWVERYRQMESNATSFEKKRQEVREKFFSVIFYGIEELKDMYQRDSKSITQLEKEIINNSETALFQYIKNLALDIEVYIHIKFNTQYKNKLEPLYVDRCKLIYLHMKDDKNLELRRKVISKEFQAQDLSTRDERDLYNPEKRKQTQEIAMRVIELNQKDNDEEQKIIKDIEEVSFSRNQSINEEIQIKTQKKDGHSSKNLLKEKMMEYSVEKSIMRFKKRIADELNDKERLQILAGLEQYQLNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UX14</accession>
    <name>A0A8S1UX14_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0500253</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8169281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UX14"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8169281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="198" mass="22776" checksum="9D84B85A43A2112E" modified="2022-10-12" version="1">MLLISVELKTVLKYWEQCLQNLKSSLKFFLTSYSFWQYSINIILVIGQQCTLSQIGVEKEATLKTKANFMSLQLLLLIRHINQKIILKLGANIHILNGILILWEVICKNLNFQLHHNTLGQIYQLKHKIVSRTVQQQIESRSQSVMIGTSYAGQYNIKIDIDITFLLVIYNKTFSQYDQMRASSIISTAPALKLSDLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UYP6</accession>
    <name>A0A8S1UYP6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Adenylate kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0510252</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000051">
        <property type="protein sequence ID" value="CAD8169795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UYP6"/>
    <dbReference type="OrthoDB" id="442176at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019205">
        <property type="term" value="F:nucleobase-containing compound kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8169795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="15450" checksum="85D4B83B6837C4E9" modified="2022-10-12" version="1">MYKFSSLRKPSVYFILGGPGSGKGTQCELLSKNLKFVHLSAGQLLRDAITSNSKHKITIENCINNGVIVPSHVTINLLDKAIFDNLSFDTFLIDGFPRNYENMQSWIDLMDHKINFKTKYDLKNIGKIKNKWKIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UZH4</accession>
    <name>A0A8S1UZH4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0530075</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UZH4"/>
    <dbReference type="OrthoDB" id="303316at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8170470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="174" mass="20621" checksum="4139802F94C12F89" modified="2022-10-12" version="1">MINSTLFSKPKQRQIYLIYASRNYTQPKPTPRTHLKLPNCSPQSRINDNKLKKKNSIFTPRIQRSQQQSPVIRQQIRIRDSSESIMKELELSGNEDLKEINKSLLLTSQEINKKMQFSIESYLNNTLKQKKINHNLNNMIDFDLTQDNNSFHSFEFSRSRRDTPLQPIKVYHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V035</accession>
    <name>A0A8S1V035_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0520237</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000052">
        <property type="protein sequence ID" value="CAD8170265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V035"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000097">
        <property type="entry name" value="Probable serine/threonine-protein kinase nek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="14"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="325"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="373"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="460"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="547"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="328"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="407"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="425"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="477"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="547"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8170265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="686" mass="79921" checksum="37C1F46764DB191D" modified="2022-10-12" version="1">MNVQQNKTSSLKDFQIIQELGKGSFSTVYKVKRIADGQEYALKKVKLGSLKQKEKENALNEVRLLASINNKYIVAYKDAFFDDESRCLCIVMELLSGGDVYRRITQALKGGPQFSEQDVWKALIHMILGLKTLHDQKIVHRDLKSANIFLSRDGTFKLGDLNVSKVAKQGFVYTQTGTPYYASPEVWRDEPYNLKSDIWSLGCVLYEMCCLQTPFRAKEMDVLFQKVQKGLYDQIPAKYSKDLAYIISLLLKTQSSSRPNCDELLQIPIIQSRMRDIETYVNNQPQNQELLKTIQIPRTIDQLNSQFPKSKYENEIMSIERNIENKSTENSSSPFRSPQQITKTQQDSLLSQGKDKQIIDITKSIAYIEKQNKHPLLVEGPRPSATRKTEKPKSAQPQLRNQKDEQGQQQLSNSNVKNSQINKYNESENLRIKRENDLKAMQEHQDLKYRELRDLKQVRDASRGINTENRRVSPISQTRQNGQYGQYQQQQEVQKQSYSPITRKVETPQYNNNRIVQSQIQSRPQINHSINISNPSLQIHHENLTSKKQQIPQSAAPNTQKYSSNQQQRPISDRYQHPQSALPSRVEQQKLERQQVFPVQARVKTKQIEQKNQSIKQTRPLSSNLIPNERLVKQEYQPQCNQIHVNYNHYYPIETYQRHASDQNLIRYQQRQQNRQVRQNIEGRRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V3K0</accession>
    <name>A0A8S1V3K0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0560044</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000056">
        <property type="protein sequence ID" value="CAD8172018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V3K0"/>
    <dbReference type="OrthoDB" id="10252354at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004708">
        <property type="term" value="F:MAP kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06623">
        <property type="entry name" value="PKc_MAPKK_plant_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000040">
        <property type="entry name" value="Dual specificity mitogen-activated protein kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013:SF9">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="64"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="373"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8172018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="507" mass="60018" checksum="4A3FAF9BE83C1E8C" modified="2022-10-12" version="1">MQSNDSNKRRLLLRSLASEEPQKISANGSLVLSQFRLNSKGLTFTKNITYMESQHFKDLKLEDFEIVCKLGQGNYGSVEKVLHKPTQDYYALKKIHYVSNDVQESLLKKELKALIDCNSQYVVQCYGAFYSKGEIYIVMEYMDMGSLQIIIEKTKKIPESITMLIIKEVLQGLDYLHTNKHIIHRDIKPHNILINKKGEVKIGDFGICSVSENSDQKFDTFIGTIQYMSPERLNGEEYGYDCDIWSVGMMTMQCITGLLPFEFNAKQMSMIEYIQMSKNFKIDDYFQQHKHTISENTIQFISKCLQQEPKDRTKAQELLQTKAIKYTQSLKIDVFKQWLQLNESTPKKSFKSRNVQISDYIPEQNRTSSAFYKRNEQTRLDQQMARYEEFKRNYKRNELQYSYYQQQPRDLNQSFVKERYQNSYNYQPNYNNKHLQTQQYEFDREAYKKEIKQDSPIKVELSYTAPCSIQRGVISDTTAYLREREKIMNSFMTQQEIETIKRIYLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V420</accession>
    <name>A0A8S1V420_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0540172</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8171203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V420"/>
    <dbReference type="OrthoDB" id="294937at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8171203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="414" mass="49362" checksum="2EE8593810370D7B" modified="2022-10-12" version="1">MKSKTIITRRLDINNDPPKQQRAPFFDPCPRFQKKYGVKTDMDRIVAYMYLRENHPNIKQCRDFSQKKICKFDSTQIKDGPPNAKIIKIQPNHSTTVNGWANVGGDLKHQFCNGWKTNIEDICIDKHYEDQLDTHFKAKEAQLKHDYKAKIEYMNKYFKEIEEMYKVNDTKESVKKLRVLYKKSREDLKPYVKYQGQRFFEVLPMMKRRTNTVIEEFKCFKPLCNLELKEKQKYLDDLFKHLNKKTEKEKDLKTKEQDSLDQQQQNEIQIDYNDSITNAPQIKRTYSQMEDVTAEAAAFNQNHYLFEVKDVNSFGQQIRMNKMNKIMNEMFKSQRNFQQDLQNYAYKHLNTSTSSDISVANISQMQQYIQNNKQKEMKRMHKSCQYVTYRTNPKSPAMKNSAFQTTATSNHFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VCL2</accession>
    <name>A0A8S1VCL2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Senescence domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0600052</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000060">
        <property type="protein sequence ID" value="CAD8173779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VCL2"/>
    <dbReference type="OrthoDB" id="291863at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009686">
        <property type="entry name" value="Senescence/spartin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045036">
        <property type="entry name" value="Spartin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21068">
        <property type="entry name" value="SPARTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21068:SF43">
        <property type="entry name" value="SPARTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06911">
        <property type="entry name" value="Senescence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Senescence" evidence="2">
        <location>
            <begin position="143"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="298"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8173779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="310" mass="34424" checksum="A2B889A46E29DB7D" modified="2022-10-12" version="1">MNNNTQAQIVTTSQNARLLELVQNSRKLVHEGQIQICYMPDARIYFVMINTYCQTIFPSTPWVILKSDKGFSLLYPNATAGLNGIFIQKGQEGDLFLNTLQQLVQIIPFENAIQIPQFKQSIQIHCKDIDINPLENSTTAVGYIKAGGEALKEGFNWLGDKIAKGVAAGGEYINSKVEKKEDVEVKPETKIKVETAKSKFSQTVDVTGAYLKQLFTPVVQKGSEIKQDINKQIDNSDSQGLKEGREIFVATWDAMGTALTGLGSALSKIGDQIGTDTRAIVEKKYGQDVSETYLGPKQQDQQQQQQQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VEU9</accession>
    <name>A0A8S1VEU9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0630224</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VEU9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8175720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="272" mass="32997" checksum="8901A3B05E4FBBCD" modified="2022-10-12" version="1">MKSRYQGTMNWDSGHQKNMMPTPTLVQSRNAGFGSSRLLLERPQVAQDLCFLLELIKEHTLQEYMEFQKQFASMNRTKRIRQEFYIISYPSIINRRYQIQSKKKIKYQIYNAISMWQVGGFLRHQNVNTNHKLELIQYINIKYPKNIIYLKMIVHKHKQVSHQLHEVLTKMYRQISQVQKVHLFFFPQLFQKGEYYYFLGQKQNLIKLFAQVFEYNIKDLALQQKRKEQQLFTISINQTIKLQQQDIFQSHDIQLKKQLLIFYIKDELELNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VFH3</accession>
    <name>A0A8S1VFH3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0630177</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VFH3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="1">
        <location>
            <begin position="236"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8175677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="411" mass="47757" checksum="CAB2B812D8711F32" modified="2022-10-12" version="1">MQQEAYCQIHREYVIQYLIPNKNSALLACAACVAGLQDEKRKVTFDNILLLHEIQNNPQKIISILANVDQNYTELSNKYSLIPEEQIKCKFQQWQNTLRQFQKWIDQLIIEYKQQLDQAIQEAQTTIKIVKKELKCDDLWNLIKQLQNQNGQIDIQDQIQCKIQIYFEDLQKNSVEIIKKIINQKENDSKNLLKKEILLPDEKEIQQKFVQQSQSILDLNMYIRRLTYSPNAPLSNIVPYDYQQQLLSLIEQRTNQKINKINQIYIGSKDGLNSQALWSKINLKQNLLFIFKSKNPGNSIFGGYANCKFNGFLNGYASDDTASSFIFSYTKNEIFPLKQEYKQSAIWCQQNGYAVYFGSNGDINISTDFINGSSDIGQSYSWNENQGGNNTYLYGGDKPNIEECEVFEIEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VGZ9</accession>
    <name>A0A8S1VGZ9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0640109</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8175981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VGZ9"/>
    <dbReference type="OrthoDB" id="291857at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12242:SF1">
        <property type="entry name" value="MYND-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12242">
        <property type="entry name" value="OS02G0130600 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8175981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="238" mass="28130" checksum="EFC9595B03D70B20" modified="2022-10-12" version="1">MKQIEQLLYEGIVNEMNQSHRVQFKYLMIFRLFASFFMTYALIQVSVVAPTFSQNFLYLTLWGGYTTYFYFGLVSIENVSFYKFNKQFFDQSLWKLCHILFIVAFCFELPIMVIYWSYIFPQENGLTINSWLINIDVHSISCILIFVDFILNDIQFQLKQSIVLIVIAIIYLIVNMFYVLVTGIQIYPGINWRNGISYIISVITLGIMFGVFQLGLLYQNKIKKPLMMNKKEKQLMPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VHS2</accession>
    <name>A0A8S1VHS2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0640240</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8176241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VHS2"/>
    <dbReference type="OrthoDB" id="346959at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="13"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8176241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="592" mass="70424" checksum="870BE1D5F7D3EBC1" modified="2022-10-12" version="1">MTESSVIKYLEQYVLFNNMQVASKGTVFRGCLLTNTTECLAIKQCELHSESESLKILDLIKMELKKLKNIKHPNLVKIYDVIKNHNMLYFIQIYCEDGSLQDYLTLRKEKPLSEHESIQFMQHIIEGYKELNKANIIHNNLKPSNILLHKGVAKISDPYYAGILQQEFMKGVAIQDLNTLRNVPFPSYLAPEILHGQPFTNKCDIWSLGLIFYQMLYGVFPWQAYSIHEWLENVSKFPLQFPPRPIRKQTLKDLITRMLTFSQEDRISFEDLIQDPILIIKQEEEQIDFQGSSDKDEFLYVLKANSILLEKNLVIRYLMKEQIVLTLEETQYQSSDVEARIQQCLFEKEPDINVERMYIKYKNDKKKKDVFYKYYQYFIFERNIAFFYNFLVQKIVKLQVDWQIQLSIDFYHRLIYLIAKYQMIVLTRISNQLQANGNHNFDQDLWGRFQKSQQFELLVAQIEQDGLNSLDFFNSITKRCQQILQEEFENNKNNQLVVGMIKNFYAVLNENFEANEIFNVLYRESIMECLKIVKNLASKQLDINQLNYYLLIALNPYDEFKDINYDFNIFYEDSENYSIQELQEKLAKKNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VNF4</accession>
    <name>A0A8S1VNF4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0670042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000067">
        <property type="protein sequence ID" value="CAD8177139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VNF4"/>
    <dbReference type="OrthoDB" id="323908at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8177139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="104" mass="12022" checksum="16B5920D8491983F" modified="2022-10-12" version="1">MLNLKTKKHSFSIQKRQYWTQNDNKLLEKVVKLHNSDSKTILLEEMEVNVHSNSLFLSIYLSKIIQLFSLASQNTFTQQGSSCYCLNGYIDEFTLDCIANITNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VQ39</accession>
    <name>A0A8S1VQ39_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0710100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000071">
        <property type="protein sequence ID" value="CAD8179147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VQ39"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8179147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="36" mass="4394" checksum="D735821F282591A3" modified="2022-10-12" version="1">MSNDKQQFQKHIIKDDNEVYQILDYTDMVELHLNKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VR54</accession>
    <name>A0A8S1VR54_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Inosine/uridine-preferring nucleoside hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0690008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VR54"/>
    <dbReference type="OrthoDB" id="432381at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016799">
        <property type="term" value="F:hydrolase activity, hydrolyzing N-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001910">
        <property type="entry name" value="Inosine/uridine_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052775">
        <property type="entry name" value="IUN_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46190:SF1">
        <property type="entry name" value="SI:CH211-201H21.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46190">
        <property type="entry name" value="SI:CH211-201H21.5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01156">
        <property type="entry name" value="IU_nuc_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inosine/uridine-preferring nucleoside hydrolase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8178152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="175" mass="19505" checksum="B0B21B2ADF5BC45A" modified="2022-10-12" version="1">MYPAIVDKLGCLFAMGGTYMGVGNAANSVAEFNVQTDVEATAAVAMGKFKQKMLLPFDVVLAYTLINKMEKSFLRIKLIQRNLNLLKVFSKLQVMMEITYFFDKLAVMVALVPSLIVYSIQKNISIVSDGQARGQVIIDWLTLLRGNQKSILSILTAFEWDSVIEIATKANQDQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VRV8</accession>
    <name>A0A8S1VRV8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0720043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000072">
        <property type="protein sequence ID" value="CAD8179441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VRV8"/>
    <dbReference type="OrthoDB" id="288302at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8179441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="79" mass="9269" checksum="0F14951F9F99BC63" modified="2022-10-12" version="1">MDNIIENSLNFWQQRLNNSSQPQPNILLKKKQRTIVDRVVIESTKYWRKMADEYSAKSSTNRSVQSLSSQIHNQQISDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VU09</accession>
    <name>A0A8S1VU09_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0760071</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000076">
        <property type="protein sequence ID" value="CAD8181328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VU09"/>
    <dbReference type="OrthoDB" id="301745at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="267"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="291"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="319"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="357"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="406"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="440"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="472"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="519"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="541"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8181328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="572" mass="68064" checksum="B6FA8B31DDA55D98" modified="2022-10-12" version="1">MQFIEFLISQLFPKPPFKNSIRPNRIKKEQEICQSLQLNWISNSNVDIIEFDSQGCRTQIKLNELYPFSQPQLRFQLDDDFYSIKNLNDYNQYDYYFKVLKWTPQLTVNQIIEKSHEFIKKNIFIKQPYTLDPYSDLLSKIAKSSKSLDLSIIFTFLLISLSNLILYLPNYEEQQQLMERTVLFGQNTWYNQFQQLPLSPLKAQFYCLIGLIVNVFDSSKLHVFKQQQEQINQPFFHYLNLIINLLIFYCPLYFLLKSKQFKINKEILQGILLYQIICPTFLGIWNIIEILMALCIFIIAENVWFGSILYVIAVNYEPQCLLFYPIISTYCLGRLCQQHGIITKKNIKPNFQGLQELVIELIFLILVLIIVQCFILIPFYFSSTLSQAIYPSTFINYFNSLFNHEFYNFIIFGFAFIFSFPALMLSFLNDKYCALGVFNVIIIIKLFDIANGITTQMLSVFLMLNFKYFRQIINYIIMLMPQQNWIFKLLYYIFGDLFMQRNNQLIDTHKHGQYQFQSLRKLGFYILLLSQIMYIYDLEMIMYKICLVILLINCLFIIQQKIVKVMKKAKTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VVE8</accession>
    <name>A0A8S1VVE8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">polynucleotide adenylyltransferase</fullName>
            <ecNumber evidence="5">2.7.7.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPENT_87.1.T0690111</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the poly(A) polymerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VVE8"/>
    <dbReference type="OrthoDB" id="412748at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000027">
        <property type="entry name" value="Poly(A) polymerase PAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007012">
        <property type="entry name" value="PolA_pol_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048840">
        <property type="entry name" value="PolA_pol_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10682">
        <property type="entry name" value="POLY A POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10682:SF10">
        <property type="entry name" value="POLYNUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04928">
        <property type="entry name" value="PAP_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20750">
        <property type="entry name" value="PAP_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Poly(A) polymerase nucleotidyltransferase" evidence="14">
        <location>
            <begin position="47"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Poly(A) polymerase central" evidence="13">
        <location>
            <begin position="232"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF20750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8178306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="554" mass="64525" checksum="BC2D1DE34FAF45A1" modified="2022-10-12" version="1">MDFSNELINFIKELAPRMSSQEINNELKKAHGRIDYDPNLMNHQCNKSQLLSFIETESLQAIEENQQKSKEIQAYLGEIVKKWSQQCAKEKRINEDHLKYCSAVLLPYGSVLLGVADSKSDIDLICIAPEFIDRREQFFNGLFYLLSNEQGMEQINKIESTSNPIIKMIYKGFHIDINFAQLNSDRVPENIDENLDELRRNLLQSEDKSMYALNGRKNGILISKSMPDHSQFVQALKIIKQWAKKRKVSSNIIGYLGGISWAILMAKICQLFPRVDVCQTVSLFFQIYSQWNWKVPVVIDESDPEIETFKTLCWKRDEDKRNDKIMQIITPAYPFFNTTFKVNKITFQTIYHEFQVGAQKLGDWNEVLKPFDFFDCFQYFVAISIVSKSSENFHKWTGFCEAQLPKLLKLVGHNEATYDTESFELRITPDGEENLERDYAKSLTYFLGIKKLKPEAWPLFLRIPITQFAIELNQRKLQNFKNEDEFNMKIYIANVTDIDQLRGKLSPRMYDDQYSIVKKIKTNPEDKNGSAYLVKRISSIQSSGINQDVLNDMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VVT8</accession>
    <name>A0A8S1VVT8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPENT_87.1.T0730062</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000073">
        <property type="protein sequence ID" value="CAD8179919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10069766at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008331">
        <property type="term" value="F:high voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000309">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000082">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000087">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000117">
        <property type="entry name" value="Voltage-gated Ca2+ channel, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000162">
        <property type="entry name" value="Voltage-gated Ca2+ channel, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000166">
        <property type="entry name" value="Voltage-gated Ca2+ channel, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050599">
        <property type="entry name" value="VDCC_alpha-1_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628:SF7">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="3" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="347"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="377"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="404"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="469"/>
            <end position="491"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="565"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1050"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1086"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1111"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1182"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1414"/>
            <end position="1432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1444"/>
            <end position="1468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1480"/>
            <end position="1501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
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            <begin position="1537"/>
            <end position="1560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
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            <begin position="1599"/>
            <end position="1619"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
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            <begin position="1639"/>
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    <feature type="transmembrane region" description="Helical" evidence="14">
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="14">
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            <begin position="1751"/>
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    <feature type="transmembrane region" description="Helical" evidence="14">
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            <begin position="1783"/>
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            <begin position="1848"/>
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    <feature type="transmembrane region" description="Helical" evidence="14">
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            <begin position="346"/>
            <end position="596"/>
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            <end position="1216"/>
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            <begin position="1717"/>
            <end position="1967"/>
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            <begin position="1"/>
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    <feature type="region of interest" description="Disordered" evidence="13">
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            <begin position="1275"/>
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            <begin position="1"/>
            <end position="11"/>
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            <begin position="35"/>
            <end position="47"/>
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            <begin position="80"/>
            <end position="95"/>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023303"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
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            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00520"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
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            <dbReference type="EMBL" id="CAD8179919.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
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    <sequence length="2239" mass="262996" checksum="CD1DB0F014E4D3A2" modified="2022-10-12" version="1">MQSPFPRKKYPSKTIVVRKNNHLFSSAEQGDKKLKSSRVSKGTRKKGNVLDNQELFQINKAPQKVQHSSESESDSDDSFQKSVLSESSKSSIRSAKTIKSKSSKRQKNNIKDLTQNLIQQYQTILRKADTQMTQQKQEEIQQKMLDRIAEQATAQMYVQPYKDSSEYESDEENSDHLSQLFSDLAKEDNKSDKSDNSIISLEVIQAQNLIPTRSIKDIQSPGTPESSIQIPNVDLSTTNVKRRISNFHSAFAGFSHLSVSEQSQSSNEIEIDQLSKLQNHYGKIYQKLSSVNGALHIKFRKMRSFSKYKLLYSSYEDAYSLLILRKIVAYFYLTTKRLSKIIVRSRIYQYLLTFVTLGNIILFIYEHLNYADQSLIAINFVFLWLYIVDIVLQLFAFGLFRSKFIFLGRVIDTFLIVMFYYHLAYTKSIINITPLRMMRLLKQLGAIFEGLQRMIEALIESLKFIFESTAVVILFVLFFASLGNSLFMGLFNERCLPSEEDEWIQCRQGECPDGKSCVMTNKAFNIPTNFNNVIYSFGLMLKTVTMDDWSWVMYYTIRAFHPIVWIYYVLIIFVGGFFGFNLPIAVFKTHFSEMQFRTISNNDEKIEAISKLQLKKIGFFYYVNQMNNYLFSKQKSILIEEPRINKLSQAWDIQPKLLLGSMINQYKIGESNFTQLKNTLKNFSLKYILLPKLNDIAKFYMSLDLKLFTQDFKQQMIIKQFSKSSFLLEKYIDYNFHYVPTSQIDILRQSMFLVLKTTENKPFSGQKKKKFYYKPIQNKNKILQNPRTSRSELTVASSQINDNKKMMLPIKMSQRIKKDNMNTSDIHGSKSSSSSNNLTQGYQMMKETEKERRKFPYFMKQLSIPLESEKKAFTQYNNGELRILVNGVYLDYSNVQKRINANIHQKNQDGPRGQEEYTQLRRKEFEKKIIKAKNWSGNNVLISSHVLIQKFDDIFKSLNQKDIEIWPKSLSGIILNFRKYAFQIIHFKLTKFLFDLTILINTLFLALQGTINRRIIDNEEDIATVVLSTEIILQLIVYQPKRIAKNKHHVIELLIVVLSLIEFGFSNYLNVSEQYLRLMRSTKCLLFYRCILYIQMARIIGAIASITYKSYIYLTFLMFFMILTFGLIGMELFANKFDEFHKEGYMQSFDDPAKAFMTVFNIMTNDDWFGVYRIGTEVQTELAVTFSIALVFTLNYFIYGIMMAILLDGFSQYLERESSNEENEFMKEKIKQINKLNKQLDSDQTDDDDSESESSNSSSSSIKQYKELANNVIVNSNRNQRRESTSPENLLASSSNVREYQQKRDSCFTEKLFHSLQFPPTRNRRPDLVNDQKKKIKDDFNRLKNKTNININHLKAQDSKKIKLIQENIEQIERQLLKQNPKLYTGNDCLQSYYIFHKSNNFRKLLFKVSQSDYTKYFIDITLLLSITYLAIPKDIQKQPLLVTLLFILNSIIFIEFIIKSIAYGVFLDKGSYLNYTWKIIDLTYIVIYYVQFFKIGYLPIFKVIETFLFFRPLKLLYRIRWVARVRAALTQSLFDIINVFVVLLLVWLMFAVFSMMLYADKLGYCEEQFNYDIGIEECQQQGKQWIVYKHNFDNITTAMPALFVISTFDGWGAILWASQNSRESHNGPHPYYSQAPTYVFYIAFCTIGSMFFLQLFTGVLFINLKANSKQIENQQLTQAQLEFKKVTEIILFDHPVRSSLPKSYIRKILATIITSKFMDFFMFSILLINCVTIMMIYHSADFHYKQIINLINHVCTIVFAIWLLLQAIALGINRFKDNPWRLYTTVVIILGVIDLLVDFIWNWFQLYVNSTQLTPYYQLLRVMFMLRNLRVIILFQGLQKLQRLVRVISFAFPFLFKILFILLIIMVVFAFYGTMLFGHIDKGEVINDLINFNNFWLAILTLFKCVSGDDFRSIMNDCMHHNPYCQEDPKYCGSPYSQIYFILFMLISNYVLLNLFVLAMIEQFELFFNNQDSILQTYVENIDTFRKAWFKFSCNHGQFLNIKNLPFLLMEIKEPLGCKSYENQWDAAKFSLQYNLISDEENRITYNQLLYEIFFHRYKKLILGQCSEIAAEKMSYYHRTMNFRLAYHRRGQKFRRSNIGPQLKLKGNCNPLYFQLIALTTFRVWRSYTQQMIQNILTEHQYFSDRTIKDTEPIRHSISLKSMESQESRIKLNDKPIYQKRRLGRLKRVSLQGQLNDYLPGDLINPKPVLKRKSSFGINVEEGNVQSVYNTVRVQKMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W2B9</accession>
    <name>A0A8S1W2B9_9CILI</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="1" type="ORF">PPENT_87.1.T0780186</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
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    <evidence type="ECO:0000313" key="1">
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            <dbReference type="Proteomes" id="UP000689195"/>
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    <sequence length="183" mass="21861" checksum="00DCA29C0A45EB2F" modified="2022-10-12" version="1">MSSANLLNHKSVLREKTLNFLNVPIAEYLTQQKPNTEDSQYKSFQRNLQLQKQILNEQDDVHHYQTELFTKSVKQERVPIIVNRPSVQHFKPTLPNDFAIPNRKSETIIKQISRIDDDLDQLAQIFYQRKLLMKTYNALEQHAERKNIQIADEHRQIQIFKAFFQQLQKKQMIQNFKKSLKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W2Q5</accession>
    <name>A0A8S1W2Q5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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        <name type="scientific">Paramecium pentaurelia</name>
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            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000082">
        <property type="protein sequence ID" value="CAD8184284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W2Q5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8184284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="46" mass="5660" checksum="64210D5D11060727" modified="2022-10-12" version="1">MKGIPYEQASERKEFVFELYDTNRYILTFHQFTFVSNRHQMSENIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W366</accession>
    <name>A0A8S1W366_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0820018</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the cytochrome b5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000082">
        <property type="protein sequence ID" value="CAD8183860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W366"/>
    <dbReference type="OrthoDB" id="302056at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080132">
        <property type="term" value="F:fatty acid 2-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014430">
        <property type="entry name" value="Scs7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12863:SF1">
        <property type="entry name" value="FATTY ACID 2-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12863">
        <property type="entry name" value="FATTY ACID HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0349">Heme</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="10">
        <location>
            <begin position="3"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="84"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="106"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="123"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8183860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="269" mass="30598" checksum="365941F447B51384" modified="2022-10-12" version="1">MLKKYSSLAQMQQESARSDRFLFAYRNNVYDLTHFVDDHPGGRFSLQTFKGKDLENILFNASIHRHQPSILSSLEQYKCGVIETKNPQDNKPKPQVIQTAKSNPPQKKEQICKTQLTDDKTNRSNVTSQSKVSIQQNSNLLSPDSNQIKKSNLKQTSKTKPINKNESNMAESTKTIKSKSDLNILNHQKFQDTSQFKGSSSQISIISNPKISLLSPQIIHEEDESQPSPLNNVEKFEGGLIPEDNINLKVPILNYQLTGYLRFKYTLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W3D0</accession>
    <name>A0A8S1W3D0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">START domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0830095</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OSBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000083">
        <property type="protein sequence ID" value="CAD8184504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W3D0"/>
    <dbReference type="OrthoDB" id="14833at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000015">
        <property type="entry name" value="Oxysterol binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000100">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF148">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="START" evidence="4">
        <location>
            <begin position="460"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="250"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="370"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="277"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8184504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1182" mass="138604" checksum="C9D099EC21FF93FA" modified="2022-10-12" version="1">MDFDFNEAQNSFQFLQVQNKLPIKMSHKLELLQECINNQVIDTSVFHSLELEECQALMKLNKQAQKEMFIQIIKGYAYANMIPSAIGEIKGIIDVRTKVLKSWKQKEFKIDSKQRTFEILRKKNSKKKPRIIYLQYYTVQPQEYKDKRYRFVIIANSEGHGYYKTLVCGSDDKALYDKVIQALKTISQFKDHVMRGSILLESNDSNQPRVINNQQSLITTRQDMKLVTQSEIIPQKQNMNIKITKQTFESDLRPNQQSQQQYQQQNLSQNMKQQSNPNQQFLQNDSPAKQQQQLQNIQYQQVQQPVISNLQQQQQQQQYQQQQQQQQQQSIYQQQYYQQQQQQQQQQQFQKNQDEQYRLQQDSLLNQQKIDKQRQQQEQLNYQLLQQEQQQKIQQQSLIQQANETCQNKNELKYPQVFQMLFEEGKFNSDYFSISNKQFTQVYCKDGVQILKDQGNSLCFRSTLTIYNAEIDKVFQCLAEIDFVKKWNAVINPNETKLLNIMQSENCGIIYERHKPYGLLYLPRDFVYLRYVTYRGDDILIVDKSIENEDAPPYMKVIRGEINYQLTEIVNNQNNIIVRIETEITNGGLSSVSQDCAITQWYLSELSHFQQFLKTYSAVQEIIQSPLLQVLKSTKVNKQKEIIIIDQGISSIQQSQQLYQPQQVQYTDINQQQQQTIPQQQQIQIQEGYQPSPVTTPSEFQFKFEQVNQTHQITLDSLPIIATVPETAPLIIENHKEINEIQQYNKDIDDADLSNYTPSDFIQQTLPELSDKEMAQVKQMIKNIEQGSYDYTVNCITHRYVQEPQRAKDEYITQINGGHYFLRQDWKRDMKHGGMIFYNQKKIEAQKRVIKFMLKSIGSNLLSGKSILNISLPVEVFESRSNLERFCYSFTFAPQILEKAAKIDNIIEQMKYTIAFGCSSIIMYMSLEKPFNPILGETYQGFINGCPVYAEQVSHHPPISAFQLKGRGYDIDGHIESAASMHANSVTGKNIGFIRVTYHNTRSKQIFIQCPGVLTGTAFGTRVFNINGRSYTIDLENNLIADMAFNPGSKNVFNKKDLLQDQFIGYLYKVKPGVLNKYKKEGYAKYTGIDFDKDVEQRYFSINGVWNQMVEFDGVKLFDTLEMNPHVMVNHPCPLPSDSNFRLDILYWKLRDFDNSQRTKEIYEIKQRNDRKWREKLTGKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W672</accession>
    <name>A0A8S1W672_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">O-acyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0760130</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Endoplasmic reticulum membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000076">
        <property type="protein sequence ID" value="CAD8181416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W672"/>
    <dbReference type="OrthoDB" id="311973at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014371">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408">
        <property type="entry name" value="STEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408:SF9">
        <property type="entry name" value="STEROL O-ACYLTRANSFERASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000439">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="244"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="352"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="386"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="415"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8181416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="444" mass="53140" checksum="48C8606ADF881068" modified="2022-10-12" version="1">MKRQKLKVDSSEVDKVSSGTSTPDEQGTHKQANIKHLRKHAFVNRVSLLDDMDKDSELNRTKFVGFYNMLYVVAFYYFIINPIMSYYKTGHLIETSLYNQMRRDLFMCIVTWPLFYAWSHIALLIQYLAMMNMPKTLMFITQHISQVCMFVYAHYLVLTRDWYLPQGAFVTFQSCVFFFKMHSYTMTNYKMRREWIDSGCPKNNDPYAYPNNINFKNFTQFIMTPVLVYEPQYPQSGQIRWKYVIVKFMNTMAMLIMGYMIVSNHIYPIIQNIKDHSLPDSIFQMTLPLIFLCLTLFNMVFENYCNFWAEITHFGDRLFYTDWWNSTDFEEFNRNWNRPVYEFLYRHVYLELIFEFGIGVKKAQLATFMFSALLHEYTLAVSLKQITPIMIMFMMIQLPVMVWTKRIKGTKFGNLFFWFGIIQGLPLILNLYLRFDELPKLLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WCB1</accession>
    <name>A0A8S1WCB1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Inhibitor of apoptosis-promoting Bax1 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0870193</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000087">
        <property type="protein sequence ID" value="CAD8186673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WCB1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006214">
        <property type="entry name" value="Bax_inhibitor_1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291">
        <property type="entry name" value="BAX INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291:SF47">
        <property type="entry name" value="TRANSMEMBRANE BAX INHIBITOR MOTIF CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01027">
        <property type="entry name" value="Bax1-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="180"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8186673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="249" mass="29445" checksum="11BB48012DE5BA10" modified="2022-10-12" version="1">MDYEQNNYQAFDIEIVDTEQQKIEYARLSFIRKVFLIILFQIGFTFITTLIAYSQYTIIDYLCNRPFLFWIFMVLLILVIFLLISFQKLAKQHPYNYICYSCFTLSISYIFFYTTHHYHTYSNHIISLITLQIGIIISLLIYSYITKQEINLNKGLIYIVFTIALLFIFLFLYFKLSLIFLFILSFLNILYGVHIIIDTLLIVNGEKHELDIDDYIIAALMTQVDIIGLISILFQKLVSQISKIQINSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WHI9</accession>
    <name>A0A8S1WHI9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0890173</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000089">
        <property type="protein sequence ID" value="CAD8187629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WHI9"/>
    <dbReference type="OrthoDB" id="337735at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20558">
        <property type="entry name" value="CYCLIN_ScPCL7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012389">
        <property type="entry name" value="Cyclin_P/U"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF108">
        <property type="entry name" value="PROTEIN CNPPD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027110">
        <property type="entry name" value="PREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0195">Cyclin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR027110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8187629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="166" mass="19779" checksum="ECE615A78956DCB3" modified="2022-10-12" version="1">MSVVMERGLEESLEDNIIYAIAKVLDEIVQETDIIESPIQTVFHTNKKPQITIYKYIERIKIYSYCSNECFILALIYIDRVQEMNSDVVINSYCVHRFMLACILLSIKYNDDDYYKNDYYSRVGGITLQELNSLEQELLTLLDYQLFVSQNQYYYYKEKLMKYAQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WHU9</accession>
    <name>A0A8S1WHU9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0910211</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000091">
        <property type="protein sequence ID" value="CAD8188561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WHU9"/>
    <dbReference type="OrthoDB" id="311218at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035829352" evidence="2">
        <location>
            <begin position="19"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="80"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="165"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="344"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8188561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="743" mass="78986" checksum="7AB9BBA6A20ABD94" modified="2022-10-12" version="1">MRIIKILLLFCIISSVFTQLPSDAPQPGQPDSSSSSPPQGAPTGLQGQQNTAGGQQPGGQQQGGMQQGGQQPGGRAKKCQPTQAQQGLQQQGQQQQQGQQQQPSQQTVPGNQLPGQQVQQQPSGTQTTQEQHGTGQFPSGTQQSGGFQQPGGVQQPGQSGGVQQPGGFQQPGGVQQPGGVQQQGGVQQPGGVQQQGGIQQQGGVQQQGGAQQPGGVQQPGGAQQPGGVQQQGGVQQSGGVQQQGGVQKPGGVQQQGGQQQQGGQYPGQQQGGQQQGQMGQGQGQMGQGQGQGQGQGQMGQGQGQMGQGQGQGQGQGQGQGQGQGQGQGQGQGQGQGQGQGQGQGQKPQGPPQSKGQWTQDSEQQQQCIEVEVTCAENEIVFKDRCVASTCENLDKVQSKQEGGKKKLPMKFDGKADFNDNKDVVVQLAFPDNKDKPLIKSDGNANACIERQIYRYRPSFLSETLDDSTVTSSYDDDKQLRIWEFTILNADIDSKLFKDEQTDKVVYTGYYSVKLTIQEKKLVQFFFTFFVAVDLDGNVLTAQATSEAQDDTPACATEDNCVSRAETEVGFCSDQTCSTLVEQPQVYAGQNFYVVQRVKQTGFEQWKVGDADVTITGDGVNKKLKPTQQQKQPGQTIYTLNIDILANNVEFSVNGKLEDPNSTRRFRFLEDASTIDGAEVSSAKAVGSTTLECIKKDANSSCPTIEEAKAYNKPDCGTIGQVECESNNNQIWSFVMFAIIMLVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WJS7</accession>
    <name>A0A8S1WJS7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0940189</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WJS7"/>
    <dbReference type="OrthoDB" id="299997at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8189763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="262" mass="29603" checksum="9FAD5BD41A552342" modified="2022-10-12" version="1">MAMLGFEQAPKSRLNNLLPRISCTGATAFRDAVMKGNQLMLQLFALFCKKGAHDKFKFVHVVLTDGEDNKSQVSLQEFLKYQAYLRQELPEDILQTFYIGVNVENNQTVQREMQAILQCSGKSASYYPIHSNGINDIFQKIQMQIGLRVQQKGLVIESDKRQIALMQKQFQPVLQMKVNNYIVLFTLDISGSMSGNWGQVCNAVSGFLGNLGSNDLVSGIVFNDKVEVVTQSNAQPQKPVQSQQYQNQYQVSNVRDEECQIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WKY2</accession>
    <name>A0A8S1WKY2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ras-GEF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0970031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000097">
        <property type="protein sequence ID" value="CAD8190618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WKY2"/>
    <dbReference type="OrthoDB" id="289012at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GEF" evidence="1">
        <location>
            <begin position="258"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8190618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="488" mass="57667" checksum="B7C70946A82E9AF1" modified="2022-10-12" version="1">MQEQNSLFFCPENEESEIIKAKTCDVQTQRIAASFYSQFSDGTDSFMDIRRNHQCNSACSSSSLQSSRTLPDECMQHNYSKCLDIDILSEAAQQLFSFAFKSQEDWLEHFIQKCIVLDTFFKDCAFNQLIEKEIQNEDCNIKKSIVLTIDYFTTLFTFSLFLIWKWIQNENQMDMINYVSCQYSIPLFFNLIFQYRFNELKNDKIFIAFIEQNIYTITKLKFEDEQQSKPYLGVGMSQNSKSLPVLKENEVFPWHPEEVAKILSVINQAFYADLQIRNVVVKGGLKQYFKRINTLSQYIIYSVVKNPCKYKRQEALSYWIDLAERLQIHNDLEGLFIVFKYGIQLLLKDYISTMPILFKHQNRISKINAYYEQQITDNLKTMKPNPKGYYIPSFQKYQTHIKRLELQAKQNRQAFEQIATLLAELVAISKDQQQRQLIMNHQLSFYEQQIARFLTQGIENELENNLKIPLEKETLVYIQLIKLSKIMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WMJ7</accession>
    <name>A0A8S1WMJ7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0940155</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WMJ7"/>
    <dbReference type="OrthoDB" id="296598at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035922453" evidence="2">
        <location>
            <begin position="17"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8189695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="247" mass="28050" checksum="4F166A2599C6E5C1" modified="2022-10-12" version="1">MKSIAILLLILALTNATNMREHKKQKETKNIKNMVELDSKFVGDAQDLSGNVGVISVTEQTNNDFVRQNNIKKAETKERNAVLNAIKKSESHKSFIQLNDNEIYVEETLDQTPKKEINFADLFTNDGSSPDMGQQALIEDSPSAPMAQLPQDENMDYVRAIDKFDLLDENGQPLQLLPEEQSLLEMKQFESRFQQDESPQMDAAPTWDLEKAYEKTPGQSELSPEELQRQTDQMVDSLKGELEDENI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WMT4</accession>
    <name>A0A8S1WMT4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0980045</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000098">
        <property type="protein sequence ID" value="CAD8191033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WMT4"/>
    <dbReference type="OrthoDB" id="301119at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="29"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="139"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="213"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8191033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="364" mass="43167" checksum="7D89FC93796D128E" modified="2022-10-12" version="1">MLVNQSTCSESATTSIQKTQDQEQMKTTIFQLEEEISGLKGQIKMWEEKYEEQERIHNREIVQLKCNLDNSIFEDQRCDENDLQVIQEETEFYQKSLGQSEVTPCKIETNNLLKILDQIEMDDTIDLQCDQETFIVYKVTKLVEMIQKMELELDDWKRNYWILEKKLNQILLQQQDKLLKQGSEEIEIEFDFDKKKLITKNQENKTIKHNDDVQVLLDEIKRLKQLNAELIQQQKSMTKQMLEQLIIKQMKSDSKNRCLTQVSEPGVKQQTVIRNNNSQHYKQLNKSPHVEPRNHYFSQYFQAQQHHQQLSMRIQPQTNNLNINQSNHSYEQPKTSTPTIYATPVMIKYTNGSPFNQANKILQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WMZ6</accession>
    <name>A0A8S1WMZ6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0980077</name>
        <name evidence="3" type="ORF">PPENT_87.1.T0980079</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000098">
        <property type="protein sequence ID" value="CAD8191093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000098">
        <property type="protein sequence ID" value="CAD8191097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WMZ6"/>
    <dbReference type="OrthoDB" id="282161at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8191093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8191097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="62" mass="7279" checksum="752C082AFDC73416" modified="2022-10-12" version="1">MYLPTFYKLGYEAWTAKTKRFMGIMPAVGCWAVWALYPNLYNTLYTDFIPPPKGVQRRVQDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WP78</accession>
    <name>A0A8S1WP78_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0960021</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000096">
        <property type="protein sequence ID" value="CAD8190255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WP78"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8190255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="180" mass="21760" checksum="EA4F882D19EFE8CC" modified="2022-10-12" version="1">MSIKQVHPTKEKEEQNEFKRDCIRVCSSVQIFNDLRKRRVKAREATQKIFQKLENFSFNFFSQQYQHILQPKVSQFTSQDLIMEEEDVLVLEGFKIKFNRAIRGLLIIMNFLLTFQIRQRKIIQLNNQLQNNKFHFSLTFNLFLENQDRFSYKAGQYIFNTSHSNLSSIINYNKRFKLFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WS21</accession>
    <name>A0A8S1WS21_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0980021</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000098">
        <property type="protein sequence ID" value="CAD8190999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WS21"/>
    <dbReference type="OrthoDB" id="302932at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8190999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="78" mass="9156" checksum="E5540976CB1554C7" modified="2022-10-12" version="1">MQVFPSKAINKFILSGDNSPAEEEISPIKITKITESRQTADLNKSKNKRKLRKSSVQEITDELLQWKYFLDHKFQHHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WSS1</accession>
    <name>A0A8S1WSS1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1000014</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000100">
        <property type="protein sequence ID" value="CAD8191797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WSS1"/>
    <dbReference type="OrthoDB" id="300394at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8191797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="121" mass="13779" checksum="56D556B566CDF547" modified="2022-10-12" version="1">MNPYPNITSVRLKCACPPSCICKSSELREWSHLSCSKYSYLSAKGDIFCNQQGCKSYFIKDASFQCQQAKQNSTFYQYKSAAQFIMALAMAIQAADLTLKDNDLIKFTTDINSEVMKRWNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WUZ0</accession>
    <name>A0A8S1WUZ0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1020064</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000102">
        <property type="protein sequence ID" value="CAD8192607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WUZ0"/>
    <dbReference type="OrthoDB" id="299117at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="14"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8192607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="553" mass="64574" checksum="D2A99C33837CFDB0" modified="2022-10-12" version="1">MNNQQRIKVSVYSYNPDDLIGSGYKSQVFKGRNDMNGEIVAIKVCDHSKMQHEIERQLLQQEITALQVLDSYNIVKMHCYCQNTNFTYIITEYCNQGDLADLIIKRGYIPENEAVLIFYQILTGFKEQISKGVIHRDLKPSNILISKGIYKIADYGFSKMVYSPKEKVYYNVGTTLYMPPQALTENKYSEKSDVWSLGVMFYQILSGKVPWQAGTEQEFVKVITTTPIQFSRNIKISSTAKDLIIKCLQLEENKRYNCNQLLEHDLFRCFQVRPSRKSVQFSLNNSQLANLENRAKTSYSQQRPNLSVQRLQSSKNLPETRQELVNYGEAVQNILNLLKLILRIAKIVDHFDFFIEGGLKEKLLYFTIKHAIFKSKQLIFILNKNHSTIDQTKKSEFLHQSNLMNDTFRISFNKLWDSIHKDETLLNEILQDKRFAAAFDQSDLEVQSHYILMLPLLRYSITYLQNQLNQKLINVREQDQLDGVEEGGILLLTYLVVYLEICMQVVSNYEKSIFYLNEFKLKLVVEEKPQNLTKRQFLQICQKIKSLQLSYLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WXL8</accession>
    <name>A0A8S1WXL8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal protein eL8/eL30/eS12/Gadd45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1040040</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WXL8"/>
    <dbReference type="OrthoDB" id="308597at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000003">
        <property type="entry name" value="60S ribosomal protein L7a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="6">
        <location>
            <begin position="117"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="201"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8193347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="259" mass="30042" checksum="4ED673BE47DD259C" modified="2022-10-12" version="1">MPKAPKTNKSKKISKKIADRKKNPLFVKEAKNFRIGNDVQPKRDLSRYVRWPRYILLHRQKKILLQRIKVPAAIHQFSKTLDKNQSSKVYSLLKKYSPETKTEKKQRLIKAAEQKTQNQKPDSKKVNVLKFGLNHVTTLVETKKAKLVLIAYDVDPIELVVWLPQLCRRQEVPFAFVKNKARLGALVHQKTATCVALTEVRKEDQAEFDNLARDLRQHYNENHELLRTIGGGQVGIKSRHQQEALKKALELEELKKTSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X5A4</accession>
    <name>A0A8S1X5A4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1140173</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000114">
        <property type="protein sequence ID" value="CAD8197213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X5A4"/>
    <dbReference type="OrthoDB" id="301136at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14811">
        <property type="entry name" value="bZIP_u2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043452">
        <property type="entry name" value="BZIP46-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22952">
        <property type="entry name" value="CAMP-RESPONSE ELEMENT BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22952:SF175">
        <property type="entry name" value="PROTEIN ABSCISIC ACID-INSENSITIVE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="6">
        <location>
            <begin position="201"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="156"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="365"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="95"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="158"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="184"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8197213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="470" mass="54800" checksum="F2A37F60052710C1" modified="2022-10-12" version="1">MADSFNSSGQFYNFVGTAGTADFLPGSGQEQQLSFGRNYLGYNDQQAGSFQNNFHQSQLIDSEVKNSLDQFFEMKIEDVRLDNLLIQEESQNQQKLKQSINSDKNSRSQYSGDQDFRLESAEQINSKFKPEVKMLIPQNQGKSIYRDKIDSLLEKRTSNNNSVSPSITPRSSKSQQKLERKRSQNLQNETSNEMTDPTQLKLAKNRESAKNSRERKKIYQQLLEKQVQELQQENEKLKDICKNQAQSMEIVNKKTQKFQLFLEQQQQMFEKLELCIIKKANFDEIGIIMDALRYRIQSNSQERNDTARVYFDSIAEIMLPMQVKYLLYACQNSKDMFANTDQDYSEWLKEGFESTNVKFENFTKLKKFQSKVQQLKQNISNSLDKIKNEIKSIQDHASKLDQVWDALKSILNPLQLGTLMCSLYHNLYRNELQTSTLFAQLKNSQAEEDDFSFKIEEEINYGTNKMVKRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X9L6</accession>
    <name>A0A8S1X9L6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1160058</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000116">
        <property type="protein sequence ID" value="CAD8197678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X9L6"/>
    <dbReference type="OrthoDB" id="79687at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="128"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8197678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="482" mass="56204" checksum="D2448D380580F62F" modified="2022-10-12" version="1">MKSPQKDFDSTQIDKKMSSFWQFKNISNLPLNISFDRFNVYSEKKKTFKVIEIAKMRSLIFKKKKNQTGYMIVDITDCIISIVENNHIGHGLSFTKSTFKFLIFGSLILDWFESLKKYCYQTEFSNKYELLNLLNRGSSYKVYEAINIYEQTNYTVKVYEKQILTKQSDIDAIKKEIQIFRLMNHPGVVNLIEVFENEQEFLIVYDSFEGGTLTDLIKKYQIPEQQAIKIMFRLLNSISYIHEQCVLHRNLKPENILVKNPLAPYNVQISDFSLADFFRRDVKYLFTRCGTPGYVAPEILQDKSYDFKVDVYSLGVIFYTMLSGGISPFPTKNYDERIFLNYHGQIDYSIIDASSDALDLLKQMLEINPQKRINSSQAIWHSVFKGLHKLKITSLESEKRNSSIKLTIKQSLPPKTIENLKLIPCPINQLPPSSKQYQRRQKFRITFKNSRQTLSNASSPMMSPGKKLFSTQSQRTLFSNFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XAH4</accession>
    <name>A0A8S1XAH4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1170011</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000117">
        <property type="protein sequence ID" value="CAD8197945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XAH4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8197945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="455" mass="53896" checksum="01EB8A8328E302DA" modified="2022-10-12" version="1">MLLLFQPLSNITISRNTEGILNFLMLIRVFLFASNLTMHQIPTIEFLKIILYILFIESFNLVYMYYRFHHIVSIYRAGCIYLINEIYSSLQNFQLVLLDEVYDYVILKIYPYKDVKNQIDAFRFCQQNRYYNNFSCNLLDNAFIEVNQVEQNVLNAQVGIIYQMMVHLAYNVLNHVKIVMAQTNAHLVNQIFDKKMNNVLDLKIFCIDNLLYYQSLDAQNCKRNEIENCLYTFEESPSQLFLNSFDYNFSPYYDITNTQCSNVKTVTILMITIKVVLKVNQKIATNFMKNINQFFLSRFVYLVHIIQILLPEHQITLYCCLIDQESLKTVDYTVKDVLFMKLVIMQKNQQVIVNYVLQNYIVQLVINRINMLKIIGKMRFVPFTELTSIHFKSSINIEFGLSKDKTDYEILCSIFEQGQGRTSQKYFKGQFKRSFIKVLKVLIFKLHSNTHYPIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XBA0</accession>
    <name>A0A8S1XBA0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">phenylalanine 4-monooxygenase</fullName>
            <ecNumber evidence="1">1.14.16.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1190039</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.16.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000119">
        <property type="protein sequence ID" value="CAD8198516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XBA0"/>
    <dbReference type="OrthoDB" id="983542at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004505">
        <property type="term" value="F:phenylalanine 4-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001273">
        <property type="entry name" value="ArAA_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018301">
        <property type="entry name" value="ArAA_hydroxylase_Fe/CU_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019774">
        <property type="entry name" value="Aromatic-AA_hydroxylase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11473">
        <property type="entry name" value="AROMATIC AMINO ACID HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11473:SF24">
        <property type="entry name" value="PHENYLALANINE-4-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00351">
        <property type="entry name" value="Biopterin_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00367">
        <property type="entry name" value="BH4_AAA_HYDROXYL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51410">
        <property type="entry name" value="BH4_AAA_HYDROXYL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biopterin-dependent aromatic amino acid hydroxylase family profile" evidence="3">
        <location>
            <begin position="96"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601273-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8198516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="421" mass="49393" checksum="189A1B529D633EF3" modified="2022-10-12" version="1">MYLNSIFKSLSRFSYIKKNFSYENLQDVEIITRDYWGSLRNILKSFSNHKINLTDIEPLKLSRTHKGQNIVVKLTFEKVEDKKYNELIFDLQQRYDQVLINNDMSVPSVPWYPRTDEDLKTIGTIMEVNEENNQDHPQFKDLEYRKRREEIAKLSQAHLIGEPVPYINYTEQEEQTWKKIYTILREKVNIVMSQRYLNNLVKIENALGFKYKIPQLRDIDAYLRAETGFRIKATHGILSQREFLNALGHRVFCCTQYIRHHSTPEYTPEPDIVHELVGHVPLFADKEVADLSQEIGILSCGAKKQDLSRLGTLYWFTLEFGAYKENGLIKGYGAGIASSIGECDHFPKAKYEKFDPFIHADRSYPIQTVQPTYMYTESFDEAMQSLIIFGKSLQKPFGLYYDFIDKELKSTRRIKTHLNDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XDB3</accession>
    <name>A0A8S1XDB3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1210083</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000121">
        <property type="protein sequence ID" value="CAD8199246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XDB3"/>
    <dbReference type="OrthoDB" id="298180at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="387"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="439"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="402"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="439"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8199246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="559" mass="65223" checksum="C9146F16CAE4420B" modified="2022-10-12" version="1">MSQKAYQSIMDANISATQLLQEEKYNQGYQILRRIETTLKQEKEAPTIPLSQINHLLSVVTNNIGCFYYMVDKFQAAEKYLKQSIEYLQLSIPDETLLITLINLVILFIKNEKYQEASRYIEQSFTELNQLQDQQLNPKIRLFLGDHSSIMTLQSLEAILLFYSGLTFYELGQSSNALQAWKKVLDILQSNTKSPYYILTKSKIQNLEAENVAKNNKGNLYSSRSHLKSDRINKILEQYSKDNEKQNQQSFRLLSQKIKKKIGNDIGEYQQPKWQNKIEHNEKKIEINNYFSDQKKIQLQINVKDLLHQKSITQRDKTRDMPLSSHSSSVNQQLNKALKKRPTQHIEIIEKLNKTHNEIYKSYLAQRSNNGSKTQRGDSNIDIRIKVNDKSNHSQNTIQQNKSHDKKSVDLRESPELCGSPQQFFNMLQSTQSVIKSQLMRSGSQGSVSQTSPFRLAQSQGEQPKNTKLPYDYSKTHRPMVDTYDKAIKELYQNQYIQIENKKFNRIYDLAQNVKNRISNINTMLIQQEKNQQILQKQFQQEFNQSQDEDSCETIISMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XDB7</accession>
    <name>A0A8S1XDB7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1200035</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000120">
        <property type="protein sequence ID" value="CAD8198853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XDB7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8198853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="95" mass="11525" checksum="1C12DD4D9B952854" modified="2022-10-12" version="1">MNYQRKSQQYVKIKCEFQFPQKLRIILLVIIMVNVQEMKFIQQEDHFISYGVEQDILQNYIQLNQILQKSSLQQSQQILEVSSLFKILKYRNQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XDP8</accession>
    <name>A0A8S1XDP8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1210109</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000121">
        <property type="protein sequence ID" value="CAD8199297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XDP8"/>
    <dbReference type="OrthoDB" id="286260at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_125, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46069">
        <property type="entry name" value="TUBULIN TYROSINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8199297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="700" mass="84225" checksum="06252058CBD9EEA7" modified="2022-10-12" version="1">MNFKIPSIHKLHTDTNSNISQPHSDFMKKQDTQPQQITPSYLFANHSFQPTLKILAPQRESIFAHRKETFINQSYRDISQRDIYGSLQKYKIEKQDDSLTQLRGRKRSIKQHQSPYNINQQSPTSIQNIESQKRILENVMDDVRLNNIKLKKLEKSPQGLNCPMHNFYYLKNMKQLYPFQIEKSVKDLMRPFHQQRENLLSPIQTERSLIYPQRPQIQRPKEFLNKLKNRLLKRKKTIFLQQTQNYEDPIQRKLKHFQKSIFENQCLHTIYIRPSAIRIQKYRIEDPNNESILKKCLSFRWWWQEAEENEEEIQFLWASEVSTSFLNKQRQRHISDKIEFQPFEQILKICEGVEDQMKFAIEQKLCLLSPYIKIHNHIDVINPLWTKKNLICRFQKYCSITNQNFCDFIPFSIIVNNLGEESFYEFIRNYKTSTEIWIVLKCQNEGEKIQLCENTKSVFNFIQKEYQTPSRKQQSFIVQQYIRSFVYQQIKLDLCYYLLITQINGIVRGYLFEQFYGEESQINFSPFEKQSKSITKLNQDYNSNKISMKTIIDYFDDCRVDYEYKILPEIKNILCEYIRSIFIQMPVKDHNFELLQVKIMIDNQYKPWLIDIKPSREFDLCQDFMKEFTQQLFDNTIQLSVDVLFPSPSIWPKEKRKLIDIYSEQNDFAVVFDSRMDGQGLKSLFEEKQPESHNDNDDEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XI11</accession>
    <name>A0A8S1XI11_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1240096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000124">
        <property type="protein sequence ID" value="CAD8200388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XI11"/>
    <dbReference type="OrthoDB" id="10407551at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8200388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="164" mass="19878" checksum="6B95E9865A43AE5A" modified="2022-10-12" version="1">MPQESIEIQMLKQQVQLLQQELQQIHQYGNIRTLWNNIFSKLETITYTSQQPLKIYQQLMFRQSLSQDQIKPEYLTQDAGCQTDDDQIKCTRHYQILYLINQYRIENKLIHLSLYQLQLLTYLKKKIILQNLSVIQNIKLGIFKLMIQNYRIKHLSRIFQIYNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XPZ5</accession>
    <name>A0A8S1XPZ5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1340038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000134">
        <property type="protein sequence ID" value="CAD8203516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XPZ5"/>
    <dbReference type="OrthoDB" id="282306at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8203516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="138" mass="16186" checksum="3C877FBCAD5E5B22" modified="2022-10-12" version="1">MQEWPKKLFLAIAFISCFTCYARPDYNLPLFAFAYLLWDIDRPVSQKIRLIYLFVYSWIIDFVWLVYWGPFWNSSTFSHNWADGIQTFVLVLSVINFIIKLGTIVVCILAEKECKDALHPENAMAHAKNIFNSEGQHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XQB7</accession>
    <name>A0A8S1XQB7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1330077</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000133">
        <property type="protein sequence ID" value="CAD8203197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XQB7"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000085">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="178"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8203197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="461" mass="51938" checksum="71AF0F310622EB44" modified="2022-10-12" version="1">MKAKASQISPDKLYASLLKKKSDQEQQARLKSPIVNPNKYLVPSNRNGVGNSKIEAKKSRPTSGKDFKDVSSVLLKKTHARQNSKLIETKENSQNISANALSILLKIKQERPETKQPVIQQNKDIFNQQSISKKYQKLMEMTKQQQQNIVKSKLFKGPRSISNPDISNLQLYITKANGLSSPGMLYNGQTKTNQDIYKLINKFNNKDNDWYIQVSDGHGTNGHQVAQFLQQVLPSFIEQGILTISSCYDRDKQVNTILKNCFLQTNEELLESGIDVTYSGATTVTVISFENILYCANIGDSRAIIGRFDNKLSVIELSKDHKPDCFLEQARIIQRGGRVQAYSDEDGNPVGPARVWKSDEDVPGLAMSRSFGDYVASQVGVICEPEIIKHALLPCDKFIIVASDGIWEFLSNEWVVETVYEYYKKDDTQGACQRLVQAAKEAWQREDEVIDDITVVIAFIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XSU4</accession>
    <name>A0A8S1XSU4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1340017</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000134">
        <property type="protein sequence ID" value="CAD8203474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XSU4"/>
    <dbReference type="OrthoDB" id="2135133at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10751">
        <property type="entry name" value="GUANYLATE BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="83"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="680"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="123"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8203474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="781" mass="91349" checksum="FBB437E86E75B09B" modified="2022-10-12" version="1">MHSTQQNKYGFQEIYKSSSNLDESDDIIIVEPLDDNQQAINKSNLLYLTAQNQSNPTSSYINRPINQSVVSSKIYSQIIPSNQPEHKPYLQQSQRPAYDPTVSVPRKYDPNLIQRAQQQQSQPKSNTNFQQPTMIEHQPRITQSIIKPTEKSFHNYNDTQQRDKNFLEQYQEYEEEDVLQNQDQLKSSYKQQSVADLPIERKQANSIIPIDKKSNLNQQPVPIRESKAPVLTEKDKQQLIANAKQQLLTLDLLQSKLSTQNYSGQDICEIVTIQKGTIKLHQDLNHILSHDRNSIVLVGVIGQNKSGKSTLLNEIAHTSNKQKFGNNGIWMLSKPLVIEQKSYYFIDCQGTDNQLLYAFVMLCSSTLLYNTLKLDDNSIQCFPVLDTILRLLINEYAIMQLLPNFIWVQRDTLQARSIIELQEFLVKANKGQQFENLIRQRDVAYIAPQGTSEYQTSIQQLKDRIILGSVCKQINSYNLNGPLMYLYMEQIVDLLNKPISLQLDQLWISICEDYTKAIYNQSLIGFVQQVENWMKDNQKVDEIEIYKQFRDFKDISLQQIFPACFLNNKNRIYQQYKKKLQEILVSKEKQAIQYCLYTSQCENDNVLNKNINKINLKDIDSFSQSFNNMIDQLQKQKYQFQQCSTFAEFLQRNYKKFIEDLFSKYQKILANTSEEQQFNLQQSKLQVSNKEDELKKKQEQVIFLEKKRNLLIKEIQDLQEQIKLERESIQNDVRQSDQGPSDDQLLELKQKQAQNKQEIQVLRKQVQQAERKKNEEGCNIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XU74</accession>
    <name>A0A8S1XU74_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1390057</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000139">
        <property type="protein sequence ID" value="CAD8204941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XU74"/>
    <dbReference type="OrthoDB" id="345735at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14003">
        <property type="entry name" value="STKc_AMPK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000956">
        <property type="entry name" value="CAMK family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF30">
        <property type="entry name" value="MATERNAL EMBRYONIC LEUCINE ZIPPER KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="104"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="452"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="452"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="474"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8204941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="526" mass="61285" checksum="DB469C1D66C28B19" modified="2022-10-12" version="1">MKHRAAANSLNQKENEKCIGNQYSYKSRPPLQQDLLMNLKNQQLRNLDAISQILQSKICKVEKRDNVEYFKFDFKQQNICDRVKFLTNQPDDDINKTEIFKAQYKVGSRIGQGAYASVRVAIQIETGTKVAIKIYEKSKIRDLQRRKGVRREIEILEKLDHPNIVKIIDTLESNNHVNIIIEYVSGSSLHHFVRKQQERRLDEDTAKTIFKQILDAVQYCHSKNIAHRDLKLENILLEGQTPKLIDFGFSTSFPIDKKVKMFCGTPSYMAPEIVTRQEYRGDKSDVWALGVVLFTMLQGVFPFKGETDAELYSKIQSGEFTIIHEISKEAIALLYGMLTTDPDERPTVIELLNYPWFKKNDQSNEKDDIKKRHKLPEDLIEDLNTITKNMSFITPTSQIKQQYYFDFSHLKNYSKPNTNKAIIPKPSYFTTTNAQAKSREPQFFKVEKILTKNDRHTTTHSKERHPTLSKERQSSYSFESSRGLSQKKNPHQLTVHPSFIPSEKSFHSFKSPQMAMNDKRFNFFYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XUK0</accession>
    <name>A0A8S1XUK0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1390106</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000139">
        <property type="protein sequence ID" value="CAD8205039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XUK0"/>
    <dbReference type="OrthoDB" id="303621at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8205039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="150" mass="17636" checksum="ECD029F524D3F3B9" modified="2022-10-12" version="1">MNIFSCPNEKEYSNDISSEIEKSQVKKELRPRNNLCPGVFQEYKRTYVKVPSNSKEQLAQLVFMEGFKIKDAAKQLNIKYATAKTIIFHMRKENIKKMNINSKNCRYTSILENRLLKYKIISTLSSRIVSSQEFIGYFQENGQDQEFKMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XVB1</accession>
    <name>A0A8S1XVB1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1350090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000135">
        <property type="protein sequence ID" value="CAD8203904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XVB1"/>
    <dbReference type="OrthoDB" id="286770at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8203904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="138" mass="16529" checksum="F947DD439B495585" modified="2022-10-12" version="1">MQQYQSQPAFNPKHLFTMKKYFNGYVQQNRFFHKKTETKSDSGSEVFKEIQQLPQIVQRIDRRIENVNYKYKSAPMIEKKKQNSRKLSFPVLKYSPTENKVQLQKIKDLIEQHTSRVIVKDDMLKHMIGYSKISQMII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XWD1</accession>
    <name>A0A8S1XWD1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1380098</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000138">
        <property type="protein sequence ID" value="CAD8204798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XWD1"/>
    <dbReference type="OrthoDB" id="311446at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050349">
        <property type="entry name" value="WD_LIS1/nudF_dynein_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44129">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="NACHT" evidence="4">
        <location>
            <begin position="736"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="107"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="572"/>
            <end position="671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8204798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="937" mass="112408" checksum="D6C8F6757ADBFEB6" modified="2022-10-12" version="1">MHFIKSIQNLRFKHLQLHLKLETIKKSFKNILEQTSPQELQKLQELINLDEFLLSIIKEYPKRIICCNSNIEKQIISELISVQFTEHDYQNQLSKQKGLIEYLQFRLSIEEQTISQEEQDLELIEKELGELFIEESPYQLRLEKIIEGLAIDESLKNVIEEKFSISEQRLEKDKFNNFLSQLRKIKDSGKYQNIHQIKIEYWDQLAIQTTTVIKTLETFGQIDKEIFQMLLNEELMNLKRLLSEEKIYSEIQLNAALQSNQEKMINEKDKITITTKSDQIQENQSHDLIVKQKENYQINEKDVITWMKSNFFSNLNRRTYLLFLIKVVKLQIQKIIESKELLQRFLEEVNVFSDKLDQIQKYKQEVQTKLQQRFQCRFQEFIKNFEQLILTQINLQQIEDENFESYFERIQTNLSKGNYDTEITQTQQNISDFLKTLKVYFSEKLLEPKYSSNSHIKQEFLIECIRKLYLLDNQEEEYDEKSDQQLGLFDTLKQKYHDFQNNEEWKIKQGLVFTIIQISSNCFTDTIISFCQKSLIQLWVSEKDLRVRTLLKNKNLINMQMQILQRDWQTQHDRIASEMQQMLKKIDQLQEQIFHEANLNKRELQLKQIDETTLQLDEYIENINEMGQQLQLITDFVNHIRKGLLRVEGKMNEMKESLINMSNEIKLLRGKSVEQLLEIRKWKVLMEAAYKNVRSIYVPLKTQEKGKNELSNLMNLEQFDDKVGEVNKFLQEEKTVLLIHGITGSGKSTTTKKIEEFIWKQHDKNKQVPNQVLIPIYISLPSLNNPNFQAIEETLRQDDYGFDDIQLKECKEMLEKKEFRFLFIMDSYDEMKLENLQKNLYINNKIKQNWSDPLVIFTTRSEILTSINYIDWFAPEDKTKLKEIQLLKFDQAQKEEYLKKFTLQSIKMLIIEMYEQQIQIENRRAMDINKFELFWEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XXZ2</accession>
    <name>A0A8S1XXZ2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1370080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000137">
        <property type="protein sequence ID" value="CAD8204454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XXZ2"/>
    <dbReference type="OrthoDB" id="298957at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8204454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="344" mass="40955" checksum="BB2475440AE361CA" modified="2022-10-12" version="1">MAIFVLQRTNKQYRQSFLFTITDNSQEMIIPLLLDDPFAYFTFDQNHLSFQIKLSNFSEFPQFNPFQQNQSNLKALLRKYVIFGTTLEDFIFNRVNTHNNKLLVNKILELNNHKKPNEKLIIKYSEFYITELMFMIVIDQKQQIILSQKQKIQKLEKGIKVFIDGINKFLQAQLNLINKSNLDRNSFYKMQTKLMYILTKFSITKDIGLSSCNINSKINYFIKLYQKAYNIQIQCQYHMDHDFYIITMKQMLNQLLIKLMTFLSKLNEDQISIEIFSEQNYIDLIIYTKSIAALNVKLQKKTNFRKILKQIGPFDKLIVNQESVIIRLYKNMGHLEDMNPFELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XZM5</accession>
    <name>A0A8S1XZM5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Vacuolar ATP synthase subunit E</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1450102</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase E subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000145">
        <property type="protein sequence ID" value="CAD8206880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XZM5"/>
    <dbReference type="OrthoDB" id="10263003at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002842">
        <property type="entry name" value="ATPase_V1_Esu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45715">
        <property type="entry name" value="ATPASE H+-TRANSPORTING V1 SUBUNIT E1A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01991">
        <property type="entry name" value="vATP-synt_E"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="29"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8206880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="250" mass="29428" checksum="045BE52DAEEB245B" modified="2022-10-12" version="1">MLQDQISLAEFVLQEAREKCFEIEVKAFKQFEKEKKQIVEKEKSNIQEEINNKFKKKAQQERIKHSALVNGARMKLMNARNQALTKIFSDSQYQIYKMIRQDERFYEELLKNLMVQGLIKLFEHEVVVRCLHRDIRHVRNVIDDAISEFQDILRKELNGLEFEVKIEVDEEKCLDERTLIDNSIKSVQDYSLQESASEVISKTENDKKCFGGILMTNKDGLIVCKNTLDVRTDQTFQDSLPIIRNMLFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y9R4</accession>
    <name>A0A8S1Y9R4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1580112</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000158">
        <property type="protein sequence ID" value="CAD8210311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y9R4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8210311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="179" mass="21954" checksum="D871974B1AC21BC1" modified="2022-10-12" version="1">MVRNQHTNIQNVFQFYYQITLLFQNLHNQNKIFYFFQVASFLNYYEMLKNKYDQFQASRGRLQFWREECRKDFSKDSKEITCVEPRVCENQKEDQIEQSLSLKLKQNRFIYYFLKVIEYLSFIRKSTCTSVQIKSQQKDCFLIQLVSIWFAQNLRQWIASLLISFFYNQPFLQKGRTDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YAS2</accession>
    <name>A0A8S1YAS2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPENT_87.1.T1510065</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDO010000151">
        <property type="protein sequence ID" value="CAD8208564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YAS2"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08215">
        <property type="entry name" value="STKc_Nek"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000097">
        <property type="entry name" value="Probable serine/threonine-protein kinase nek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000172">
        <property type="entry name" value="serine/threonine-protein kinase Nek1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="10"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="341"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="596"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="342"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="366"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="415"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="428"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="455"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="479"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="517"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="546"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="603"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="636"/>
            <end position="663"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8208564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="832" mass="97964" checksum="D3A89E2555E2E7F5" modified="2022-10-12" version="1">MRKQAEYELYKRLKLLGEGSFGKAYLVECLQDKSLWVTKYMDLAAMTPQEKDETLRESKILEFLSHPNIVKFREVYKTKKGRLCIVMEYADGGDLSQKIKEAKGKQLPEAQILDWFTQICLAIKHVHDRKIIHRDLKGQNIFLTKEGQVKLGDFGIARILKKTVEKAKTMVGTPYYISPEIIEGKPYTFMTDIWSLGVILYELCALQPPFNAESLHFLALNIVKGQYKPIPANYSKELKQLVQSLLQVDYRRRPTIQEILKMPVITNRIKSFLSETIQRQEFSHTVFHNKVFEVKGNLNQVNLILNEQPCPPQLLEKNAKNLQEFPDIVKKPSIQEFDAVRPPQLSKQQQQPSSRQSDPQKETPKIIQQEQQRQQDFQKQIDVPRKIDPITKNSPLLQKKDISKVTPPQKEIKKQSPQQQQYPSRPNSDQEKQQRSEPCKFDAQDQKKHSNSSEQPKHQQKIDQPRPPTAPKEKPKIQVAQQRPQSSKPQIQEQKVVQKVNIEKKRPISVDKSPVIQQQQQKQLQLEQQRQEDRNRQLKLKQQQEQQEKERKEKREKELQIQQEQQLERQRYFEKQQQLEKQQQLEKQQQLEKQKQQQQQLLEKQKEQDRIKQEEKQKNVQNQQVSKMDINRNEQQKQNLERQPSQKSSQLEGKQNESKSQLKSKADQLQKIEKNGKDFALMLKELEGLINNVHNAIDKFPSQGGKENYFDFSSIQTLQLEDREDDEDEPINQFDRKPKYIIKQISKEEEECLEMLNKQDSNLSTLKIKLENALGTEKMNKAKNILKQTLEKMDLETMEQQYGPNYEKLLPFLTLEERKKYAPLLFTYIIAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YJE2</accession>
    <name>A0A8S1YJE2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cyclin-dependent kinase 2 homolog</fullName>
            <ecNumber evidence="2">2.7.11.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cell division control protein 2 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">cdc2-related kinase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPENT_87.1.T1620123</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">May form a complex composed of at least the catalytic subunit CRK2 and a cyclin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000162">
        <property type="protein sequence ID" value="CAD8211264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YJE2"/>
    <dbReference type="OrthoDB" id="290764at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010389">
        <property type="term" value="P:regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF254">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="7"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8211264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="293" mass="34062" checksum="CF19E600103547E4" modified="2022-10-12" version="1">MNSIQQYEKCENLGQGTYGKVYKGINKLTHEVVAIKEIIHSEEEKKEGIQSTTLREMSVLLQFRKHPHIVELKQVILDDTDYKQYLIMEYIDTDLRKMIDQKKITEHMAKKLLLHILRGLYALHSQLIFHRDLKPANILVQNGVAKLGDFGLAKPIGYPINRTHTKEVQTLWYRAPELLLGHFKYSPALDIFSLGCIFYEMLIGEALFKGNSEIDQLIRIFQFMGTPLDWEGFKSMPYYSAKFPQFKSEAEKHLSNYNISQEAKNLLLKMLQIDSTKRITAKEAINDIYFSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YJF8</accession>
    <name>A0A8S1YJF8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1830029</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000183">
        <property type="protein sequence ID" value="CAD8213658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YJF8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8213658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="181" mass="22138" checksum="30F1001900988528" modified="2022-10-12" version="1">MQYQVLYAHKTRENFCEWDYQGKKWKLKQQYQEYRIICLLKATSELPEQKAPLEFRHPKFVKWEEEFKKKFFIWNDLAKRFHKFIQLKKVGQENTQNNPNRINGRRQTKQTMKEVQQINRDDIREVTKNIRINQEVFHLECLSLDKSIPKLINRLTDKEIRIQLNGDGLFKQKKVQLIKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RY05</accession>
    <name>A0A8S1RY05_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0020151</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8132948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RY05"/>
    <dbReference type="OrthoDB" id="45518at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8132948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="82" mass="9850" checksum="46361888FABC938C" modified="2022-10-12" version="1">MLATKYIENFSDDQLTCFIIYNDTKAVSNIPFIHKLRKITYVSIEILLISQDVIRKSIEKDEQYEIKDKFMNQQKNNNVKRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RZZ9</accession>
    <name>A0A8S1RZZ9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0020459</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RZZ9"/>
    <dbReference type="OrthoDB" id="75724at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46818:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_125, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46818">
        <property type="entry name" value="DOMAIN-CONTAINING PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="112"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8133546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="339" mass="39401" checksum="A190825046B6F287" modified="2022-10-12" version="1">MQQQQVVLNFDYLRPAQNLIDAIVHDAQYIKRKERSKSQRRIWENVPYDAYEKEKMGDFMKIVTKNKVVLPQNWTDSDTLKMVYCGKFKDKNYLKVLQSHLAWRAIPNNFQPTDINIAFLQKGIVYTHGRDKQQRPIIIMNLELVNLKQFSEEVYINALSYYFGIIKKYCFVPGKIENWVFIMDTKKLGLSKFPFKAIQIATKTMQVNFCGCLDKLYLLNPSSSLSFSWKMVSAVADADTMEKVQMLKPNEYNKIQDRIASNQLEEQFGGTSPNLTKFWPPINIAIPGGYTEEILKAVESMQTAEIVIQKEVPASEEDEMKLQEQISKMQIKKDDDDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S0P7</accession>
    <name>A0A8S1S0P7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VPS9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0020245</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S0P7"/>
    <dbReference type="OrthoDB" id="300856at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003123">
        <property type="entry name" value="VPS9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51205">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VPS9" evidence="2">
        <location>
            <begin position="261"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="63"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8133130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="387" mass="45556" checksum="1260F145459437A7" modified="2022-10-12" version="1">MGNSQQKNDDKLISTVWLGVNNDWKSLMKDRLSRLEEEWVISMIKCLAKHSPQEQNRFRHKIKQVLNESLKEEEMKKIQQQSEEFLKNNEFLREDTCIQTSSSEQTLSTSYNSFSKINENQSISSYKQDLQDYETQNLQAQKFYLVFTIINGMIKSNKHPFFYMITQFQEWAKLHLNGLQQSNQNMSQLKAQVAKFGVTTNLFLNYLIHALNEYLGDISNDQFDVAQNKYLKDKANYFNLFLSITMNETLTIQLMEVLKILHKNSIINLMGKMTHLSLKPDFFEIPKKYLTQNKPFQEQIEYLQAIQYSKHPAEKYYVLAKGYQLLREQLTKSASNFVQLQATNYVLVHSQIQNLHAHLHLLELFYPSSPLLLKYNSSLQFLQNIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S1Z5</accession>
    <name>A0A8S1S1Z5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0030218</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S1Z5"/>
    <dbReference type="OrthoDB" id="270318at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002060">
        <property type="entry name" value="Squ/phyt_synthse"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00494">
        <property type="entry name" value="SQS_PSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8134268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="314" mass="37231" checksum="26CC095041509C01" modified="2022-10-12" version="1">MNKLQFVFKFCTESQKSIYKLENAQKYISDLVQKTDWISFAISKHIPKQVRQDYLNICQFHNELARIEETSKERSLGVGKLQYWSDQIDQIFLDNPQRDPLSICLHNSCKNNPLPKYLFQKMINHRKQQVERSEFANLVDMATAAEVNRGSQFLLFLRLMRMDIENQDLKSLTEYSAQAVGITDYLRRVPFTLQNYKLNLPEDIKIKHSINVRSLWDRIRGEPKEELYDAVLEVAALARTQMINANKFSNKIPAQSFRAFLHLIPTEQYLEELEYYNFKIFEKAINQNSYVQLPITLFKNCRKMRFLNKDYESQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S5R4</accession>
    <name>A0A8S1S5R4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HOOK N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0030388</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S5R4"/>
    <dbReference type="OrthoDB" id="296466at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030705">
        <property type="term" value="P:cytoskeleton-dependent intracellular transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043936">
        <property type="entry name" value="HOOK_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19047">
        <property type="entry name" value="HOOK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="HOOK N-terminal" evidence="2">
        <location>
            <begin position="5"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8134600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="123" mass="14558" checksum="1CBE57A99EAA428C" modified="2022-10-12" version="1">MQNQELLSAIVDWMNKITFLKYHTNFKEYVDGTLFIQILNHINTDHFGSIFHVLENQSYIDNWALNMSRLKLIVDKLTDYTHKQLDLNVMEIAKDKNETQILKLFGIICQVFIISMFLANFIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-02-22" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S682</accession>
    <name>A0A8S1S682_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0040050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="12"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="124"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8135093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="248" mass="29394" checksum="7B0935B29D36C5E8" modified="2022-10-12" version="1">MQSLFNEDAXELQNWKNQCRSKDQELAMLRDQIQDLQDANSKLSMELSSLKLIIQQKDNELEKYRITSLELRSAASESRDAQSRIRELEMEVNNLKSLLYQRTTECEDLRNQLALNDKEYSSKLNQYRFQISDLEQEILRLKTEIEKLRQMLANKDHEIDDLHLRIQYLEEQGTTVVQEKVTFLSSEIEVWKQKFIKINHDYNECQEQLMMAQAELEALQKQQKKEVVVEKRTVTRSTVGSSSIQKQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SEX1</accession>
    <name>A0A8S1SEX1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0080241</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SEX1"/>
    <dbReference type="OrthoDB" id="630188at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07379">
        <property type="entry name" value="MPP_239FB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051693">
        <property type="entry name" value="UPF0046_metallophosphoest"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12905:SF0">
        <property type="entry name" value="CALCINEURIN-LIKE PHOSPHOESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12905">
        <property type="entry name" value="METALLOPHOSPHOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="1">
        <location>
            <begin position="22"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8139681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="235" mass="27231" checksum="978020E789D1EB66" modified="2022-10-12" version="1">MENDLTFVCISDTHCCNIPLPPGDVLIHCGDFTKRGQKEETLSFIEWLIKQPFKYKVVIAGNHDISLDTENYRSKLRDYHHKVSNFNDEELLQKLKDNCIYLLNSSIVIDGIKIWGSPYSLEFCTWAFQLKNENAEVFWSQIEEDSDIIVTHGPPLNHGDLVDNWGNKKHVGDESLLKRVLQIKPKYHLFGHIHEGYGKTEQEGVNFINCSLVNRNYKVVNEPFIFKLPKKQVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SL54</accession>
    <name>A0A8S1SL54_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0090126</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SL54"/>
    <dbReference type="OrthoDB" id="305190at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="248"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8140540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="666" mass="78746" checksum="77CA2BAECBE98900" modified="2022-10-12" version="1">MQNFEGETLVRQLISDISRPIVEKLENVQKEFENIKLFLHQHNRKIDILSDQSDQLSFDIKLNNDVIEQLRKNTIEDQQKVFEIENHLNLEFNKTRLEQGALKLEQDSLKKNINILGDVQQSLTERIEIYNNYLSQKVYQQEFAISNVDTSNSKIIKQLTDINLQNQDEIQTQKIQTQDILSKLQHNSQELNKIICSQQWMQDMIVELRQKSQFYVTIDQIDLVQQNPLKNLPSIITENCQISNQVIKEDFQETIDEKFKELKKYVDKQIQQSKEQNSIQIEELQKALATLKQDFVRRLQQTNSNLKDTNEKLSTQITVVQDQKLNSKQQISQFNEQIQKQFLKVSQDIQTIQTNITGLDLRMAETHLKVVHVQEKQQQSQVDQTTFEYFNNQNEIISLLPIKQDSQYSDSFQFNNAVTQKFIKADQSIQFQPTQSIGTKKQREIQLFESDQAQTVIQNLDHQSNITTPHIKVQNEIIKLKSDFFNEQQYNQNQMNNLLKQKTITDQTLQEIDKQLKQFNQYFGVIFSLILHNELIGTDYQAKILGNGFKFEFLPFQQDERTIISYQNNKISKIDLLMKTIISTYSIIKKQRQNTLEFQQEKKRHNTETSRDLQTMYPNKKNRFLDDKQYITQTERLNLSLDNTAYQQLPKQKVKKVLVKYKKLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SQ37</accession>
    <name>A0A8S1SQ37_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0090127</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SQ37"/>
    <dbReference type="OrthoDB" id="288268at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000198">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF803">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="9">
        <location>
            <begin position="605"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="75"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="420"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="576"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="96"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="120"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="425"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="436"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="460"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="581"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8140542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="892" mass="103383" checksum="556202646EFBFB9F" modified="2022-10-12" version="1">MGSGASIEQEQQNLNLPAQNNIFIDEGVGIEQSQLSRKWTTKMTMFKNLETKNFMESQFQNKSPKQTIKLEQKSSVVYSDDSDQISKSSSPSVKSSKMHFPHQKKKNSFHTDSEEESDIEEKQLYNDRRQTKFQNFQSDDSFEERDDNTKKIKQLIYQAEENITSRKSSINSRSDQQFLANFEIKDIAETINLRQLSLPHQYPLCQLYIRTQISEQKLLPGKDKLVKLSCRQNLQDSESEDSIKNQNDSKSEIQTDYSPEQLLNQIGIAVISKKGNKNDTAPLAQDYLIYNDNYQKILILSNGHGEFGEKISNLTYRMVEFQSVQFSLQVILQQICQQSIINIKQIDLWVVVLVQNKTQNNIFIDEGVGIEQSQLSRKWTTKMTMFKNLETKNFMESQFQNKSPKQTIKLEQKSSVVYSDDSDQISKSSSPSVKSSKMHFPHQKKKNSFHTDSEEESDIEEKQLYNDRRQTKFQNFQSDDSFEERDDNTKKIKQLIYQAEENITSRKSSINSRSDQQFLANFEIKDIAETINLRQLSLPHQYPLCQLYIRTQISEQKLLPGKDKLVKLSCRQNLQDSESEDSIKNQNDSKSEIQTDYSPEQLLNQIGIAVISKKGNKNDTAPLAQDYLIYNDNYQKILILSNGHGEFGEKISNLTYRMVFHYLIRTSTFYTNPMRGLETIFKKLQNKLKMHIEKKKLDDTHNILLSGCVITVIIQRDSKLYCAQIGDNRTYIYKEQVNQNRESNIIAQTLPIHTPNESSEKARIFNSGGEVRKIPQGEEYVFVRGRLYPQLHVTRSLGDVIAHVIGVSSQPFFREYDITIKDTFLILSTASVYAYQDENDVMNHLSGFSLHDIKSACESLYKQCKNSWILNEGMFEDMTIILLWLVQQKEPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SQY7</accession>
    <name>A0A8S1SQY7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0090345</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SQY7"/>
    <dbReference type="OrthoDB" id="10298099at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8140914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="62" mass="7373" checksum="3921A30620CEBE4D" modified="2022-10-12" version="1">MISNEVLFWIKNPVSIAGENINGICYLRLIDDIQHANVQLIFITIEYSKILHKRQVIIYFYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SSM8</accession>
    <name>A0A8S1SSM8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0110397</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SSM8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8142908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="40" mass="4842" checksum="96CC477145B9B067" modified="2022-10-12" version="1">MEYDMQNSEKIIQPQNQGQYKENPFQINDLRKLLKKKSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SUX5</accession>
    <name>A0A8S1SUX5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L37a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0120299</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SUX5"/>
    <dbReference type="OrthoDB" id="308782at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002674">
        <property type="entry name" value="Ribosomal_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050522">
        <property type="entry name" value="Ribosomal_protein_eL43"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00280">
        <property type="entry name" value="eL43_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L37A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01780">
        <property type="entry name" value="Ribosomal_L37ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8143578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="15483" checksum="660EC7F50D65FF9D" modified="2022-10-12" version="1">MTKKTKKVGITGKYGTRYGASLRKIIKKFEISQHQRYFNTFTGAHSLKRQAIGIWRCTQTGLQIAGGAWEVNTPAGLSAKQGMLRIKKLKEDAEVEVKDEKKEQKKQQPKEQQKEQPKEKETKKPQAKKQQAKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SY69</accession>
    <name>A0A8S1SY69_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0150397</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SY69"/>
    <dbReference type="OrthoDB" id="301940at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="594"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="14"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="143"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="461"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="594"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8146541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="750" mass="88977" checksum="C6A41299A49EFF34" modified="2022-10-12" version="1">MKLKNRNSTSVNKLQNLKQQQDNIIQSINELNDKNQQIIKDYMNKQQQLTDQLQKQHHQITIQELKQALQNNVWTQKVGFFLNPLSQQIGQRITIQQQQILLKHSESKLILLERIFQTEFKIVQFQEQKKMIQNDIEGRILQMDTLLIEYEELEQIIDSQQNRSQCNNIKTIFEINKNLSQLKHNYIQLANQRQEQEILQTDFLQNINTLELQFKSIQIEKQMKGLQSSWFNVDQLYNLIVTNVKSEQQLDLFCNIVLLINDKIVQGLNSKTNQYVIIENIRQIENDNNFRVFNSTIFQEYLDQAQIIEYSQFLKTELPKRIYKFKIESQLKQHKTHLIDIQRQIHQIMYEQSMIQTQYNRLESNLPVLEQNQTAQRLQQILDRMNHIECLKSQNKTLFEITFPAQIKQIQDNLMQMKQQLNSQIKDLQYSIGIEQDYYYKIFSTQSQKLPILILDSNIVNNNIEQQIEENRKQYQQQQILYNQNVENLRQKLKQLEDQENQMQTRYLIELNESIMKTKKENIPPSDSRDMSILGTIQIDMSQERTTHKYSVLRSKKQLFMNEPTDRSLSTYSFLGHRSSDPLCSKTKLALKQQTSLSSQTSQKNPLLSPQSNNHHISIEQSLSLHSSARRFPDFQMEKSPPPQQIIGEKIFKVLKRTFRQQSTNNKNGCSAFSSKFVKYNQKMKCLEFYNSNKIIHDLGQKESALCLKQIKEVKQCQQSLVLVISKHQSIELVFNKHEQLNEFMKMITQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T0M8</accession>
    <name>A0A8S1T0M8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0150154</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T0M8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8146060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="169" mass="20039" checksum="891176B00E5BC634" modified="2022-10-12" version="1">MLIQAMINKTIISNKRNRKISLQLKFKKQKDQKQVDDTLMKYLTEHQGYNLIQFGIVLTLFNKFHLKLKFLSVLQIFFKGSNIVGSKFKIDKLICEGDGNGVNNVKKLLFQIKKEKLNFNFIKMVLLFVLVIQCQKIQIQLNEQLIQLTLKIKYNLVQFGWQNYFMFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T4A3</accession>
    <name>A0A8S1T4A3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0180152</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T4A3"/>
    <dbReference type="OrthoDB" id="307925at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35381">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35381:SF1">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="523"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="260"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="537"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8148611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="547" mass="64457" checksum="E17AB1B61B18D034" modified="2022-10-12" version="1">MIDQESLLAELGLLDNRKLLLELTIQLGQNEHDIIYLYEGDDIEVKASQFCEKHHLKDEIKDVIAQNIKLNLNEKENYFSFVNQSTQISNTNTNKPLQDQAIKQIKQVNQEKKENKNDNLNKSQNGIKKTDIYEKQVKYMNYKKNAIQKQRILKEEESIREATFKPKLNQNTLKIVSTIRQNRNNTPEYYLMKYGQQLQEQKKKAEQQLLNETSRECSFRPEINKISQKICEEIIKKDVSMTKFDQLYNQAKKRKANDKSQTSIIQESQTQLTKRSVTPNKSQNKQYLPFLERMEMQKKIKEDKLKQDIEESLMYDDKSGQKLFQPQVHSANRSRGGLNIGEYLYQQQKSLNEESIIQFEYDDLSKSIQTDRSNQIVQQMRRKKLEEYFKLMDSDNDGLISAQNIYLEELPTEALELLQPVILQLEKLDILLNIETWTAKCLDIIEKMSVFDKNRLFQKQPKQQKTQPDSSSTFRPQLNQKSEIIAKQRQTFKGPAALYQKAMKENIVKEQKIKQIKEQQEQKEAKQCTFQPKIKLSNRSTTSTSFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T746</accession>
    <name>A0A8S1T746_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fatty acid hydroxylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0160090</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8146864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T746"/>
    <dbReference type="OrthoDB" id="1658724at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="66"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="6">
        <location>
            <begin position="167"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8146864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="326" mass="38636" checksum="1B19F2493D266640" modified="2022-10-12" version="1">MDSLNDSCVSSKDINDGILQREESRYQRKGYGLIFAVLFTLFGFTVFPQLIQFRILSLLQNLQPQYIQMIMLISTHLLGFSLGNLGMYFIYEAKSPFFERYRTRQNPWPWDENPDEWQKKRKEVWKYSYINMTLGVLMCILDTQISPLLRNDIDTFPSLFEMTWQILFSMLIEDTCFYWTHRTLHSPKLYPIIHKKHHEFYTSVSYAAIYTHPIEYIFGNVIPVFVGFKLLGNQMHIATLQLWLFFRIGETIDGHSGYEFSWSPYRLLPFSSSAESHNYHHSHNVGNYGSFFIFWDTIMGSNKSYNSFIAEKYGKINKIKDHNKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T8G5</accession>
    <name>A0A8S1T8G5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PhoD-like phosphatase metallophosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0170083</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T8G5"/>
    <dbReference type="OrthoDB" id="10266805at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07389">
        <property type="entry name" value="MPP_PhoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018946">
        <property type="entry name" value="PhoD-like_MPP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33987">
        <property type="entry name" value="CALCINEURIN-LIKE METALLO-PHOSPHOESTERASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33987:SF1">
        <property type="entry name" value="CALCINEURIN-LIKE METALLO-PHOSPHOESTERASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09423">
        <property type="entry name" value="PhoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035761109" description="PhoD-like phosphatase metallophosphatase domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="376"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="PhoD-like phosphatase metallophosphatase" evidence="3">
        <location>
            <begin position="26"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8147676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="421" mass="49918" checksum="B838EB8B1E2A2E25" modified="2022-10-12" version="1">MFNKLLIVLLTTQFYTVTAEKQLTIAFGSCFKFYRTHDTDVFQRIKSFNPKYFLWLGDAAYIDYMPILGIWRAEQNETRVKEKFDITNDDKYYAQFKKSVIIKGVYDDHDSNENDGGKFNPLKESAKQLYLDFIGEPKDSPLRQQDGIYQSFYADQYNKILIVMTDTRYNSDKYTGDSLGENQWKWLEQQFQTESQLIIMTSGVQVMHDDRDGPETWFGWSKKRLFALIKKYNKPIIFLSGDVHYSEIMKYPCPHRLGQNLYEFTSSGMTFANYDHIPFVGLIFQFLFPTTFSNYQDHYYKSNFGILKIITNDQHSPIRIEFETHSSEDSSIVLQKTIQIEELQKQYYDESQSCILDVSIKERKWQNYLLRINENIIIVTGSLFVALLFIIFLIYNFISYISKFIELYKQIQKNKQKIKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TGE8</accession>
    <name>A0A8S1TGE8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Arrestin C-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0200101</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TGE8"/>
    <dbReference type="OrthoDB" id="296266at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF17">
        <property type="entry name" value="FI21816P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="1">
        <location>
            <begin position="191"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8150122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="360" mass="42379" checksum="ED9EBC1DB422E55B" modified="2022-10-12" version="1">MIFSKPIYNQYLLQQSILKFYINDKQYIKLYLIPYDVNNPQMIPEKLLEKNKQIQLTLDSPRNFKQTQINPKKKYELHNGTMLQITRQYGVNEDYIHIQDLYRGYIKSGDYQLPFQVPTKFGMNSSFQYRNLDGFQQAKIQYKIYLKISDSQSNQVIIQKSIPIYLNSRYKLIEQKRESEGNIVQFFCLHRGMIELSIRTARNLYKPGDVLELEYILNTTRSQRSITKVEVKLNHFLSLTDDDENERVIENNTLYTNVLPGISPGKRSDGLKSLITLPNNLTATVKMQFIKNHYILLVQAFADGLLTSLAIPVICQIPIIILESRKEEKPILNEWKFNTQQQQNNNSFYQFTCQQQQLFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TGJ7</accession>
    <name>A0A8S1TGJ7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0210024</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8150804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TGJ7"/>
    <dbReference type="OrthoDB" id="297743at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8150804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="289" mass="34227" checksum="9D388F847441E082" modified="2022-10-12" version="1">MNNNLKSTYNKISDVFSNSQIKTAVESLKTYKDRLKLIDSIIDRRQEIIKANDFSKIQKCAHTFSKIVKKYKNPDEDDNTINQRFQMQMVINQFLQKNIQNSLSPLSSPQSIQNFVTQTLSHKKDFTTKQVSQRRSRYFSSQELFNHQNKQLDIMSDEDRDIQSLKSKIQQSLILKTRIKHDQNHKDDQNNLKLYLKQQGYFRKNKIKQCSEPILSSLGESQQYCLSKQNQINIKGIQEANNNNLNSIQLNRNFQQFDEKEIQIKENKKYQKKREIFDKSNIILCSFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TK34</accession>
    <name>A0A8S1TK34_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0220371</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8152283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TK34"/>
    <dbReference type="OrthoDB" id="291440at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8152283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="137" mass="16132" checksum="E36CE10A8CDDABB4" modified="2022-10-12" version="1">MQQISTKEGKYLYGLYVLCQGNIISSSQKGYLKDLLIQKDQQILEIVNEFNKEECLQELQTKLQEILIDLSSESQSLAGNTPIHQQWCYRSQDNNIWVLNQLGIKESQMRYSYKFLETQTSSQKRDTPREELEDQFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TLC4</accession>
    <name>A0A8S1TLC4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0260164</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8154831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TLC4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8154831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="200" mass="23731" checksum="8E4AE92ED9BD4803" modified="2022-10-12" version="1">MQSVSQISKSDPTRYGDSFKPTAKKMTHRLLSHCDSEMTLKKMDFTLAGSGKHIPDYNIKSTQVETKQIEPFKIKDFIPQINPTIKGELSRLNKNSFLDFLLIQKAGKSGDQKFFDQLIQQYNFDPFKKTLKMQDNKQQQIQYLQKRDEREEQIALQVKQFYKHMQHKRTIRPYDHRKKQLFDPKTTTLQSQIRGFVRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TNT9</accession>
    <name>A0A8S1TNT9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0240340</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TNT9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8153663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="92" mass="10985" checksum="3BAD01AB4A40538D" modified="2022-10-12" version="1">MGKKFHNYYCIFFQEDVECNKYASYSQGLVHISQKMQFKLEKKIINNIEKVAAIKKNYQQREGRQYDPSFLRDLSLIQQQKNTISIITQQIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TQ26</accession>
    <name>A0A8S1TQ26_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0260151</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8154805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TQ26"/>
    <dbReference type="OrthoDB" id="300207at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035754033" evidence="2">
        <location>
            <begin position="17"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="291"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8154805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="343" mass="38487" checksum="EBC7EADE5D2F1A01" modified="2022-10-12" version="1">MKLTLALVLLVTLASATSTHDQIMAFLQTGTKAMDAIDTVFGLLNDLIQSNKDAQFAADQKNETDEWVGAQTIEQFTKIKTLNQKLFQQSIENRAQFEQELSDTKNYLAWNEQRQDEIARKIDVLLDQQCLSNQLFVRSIKQNREALEVVRLLKQDVAGYIINGDSFELVQVQSVAEKLKSYSNTFNEHEINTFLQLANNQEDGSVSRGATLAERVLSVLEGLEANLAASLEALETNEINASWELAGWVSLSEAEIATLKVEYERKQVYADRLATQIQAALAQQAKSKIILQESQDALDQAQADLEAKRADYAEAKAKRDEENAIIEQVIIIFKKQVASWSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TRG0</accession>
    <name>A0A8S1TRG0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0270031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TRG0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8155325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="186" mass="22843" checksum="93D34E02F69833D1" modified="2022-10-12" version="1">MINEELAQQINQQIQLKKKQPCELEILRKQKIQNFVANNLVCKIIILELQEVILNINNFRKQEQFFLFLFQKLDLQFQQITIKELFQFFMSINKKMILILYNISKKCLVYDDFIQSNKYKILLRGQIKKLQNFKYCHQLHILMKIIWKNSQQFFLIIYLYLIVKKIELAWMYQKQLAISYLLQYKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TV94</accession>
    <name>A0A8S1TV94_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0280155</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8156150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TV94"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="23"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8156150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="296" mass="34038" checksum="3BC9F905C7AC8378" modified="2022-10-12" version="1">MKQNLEEIAATIPNYKMVDGKYYIKTTKLGKGNFADTHLATLKDNEKQVFACKLIAKQNIIEKLKKSNNPESRKEYIINSLKIEVSLWKQMDHPNIVKFIDFSETPNNIYFFLEYCNGGDLDMLIKDKGKLNEQETVDIFLQIAEGCSYLYDKSVFHRDLKPENILIHNGTAKLADFGFAKVIEEDKKDVAAHGTSVGTPYYMAPQILAGEDYCIKCDVWSLGVILYQMLFGVLPWKDTNSIMLLLNAINNQKIQFPNTNQVSQEMKELITKMLQKKEEDRINIKEVCECLNKIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TWZ1</accession>
    <name>A0A8S1TWZ1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial</fullName>
            <ecNumber evidence="20">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PPENT_87.1.T0290077</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="15 20">Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 20">
            <text>a quinone + succinate = fumarate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:40523"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="18">Flavinylated by SdhE, about 5% flavinylation occurs in the absence of SdhE.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 20">Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 20">Mitochondrion inner membrane</location>
            <topology evidence="1 20">Peripheral membrane protein</topology>
            <orientation evidence="1 20">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="20"/>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TWZ1"/>
    <dbReference type="OrthoDB" id="71672at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.700.10:FF:000001">
        <property type="entry name" value="Mitochondrial succinate dehydrogenase flavoprotein subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.80.40:FF:000002">
        <property type="entry name" value="Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000482">
        <property type="entry name" value="Succinate dehydrogenase complex, subunit A, flavoprotein (Fp)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.100:FF:000001">
        <property type="entry name" value="Succinate dehydrogenase flavoprotein subunit (SdhA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003952">
        <property type="entry name" value="FRD_SDH_FAD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011281">
        <property type="entry name" value="Succ_DH_flav_su_fwd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014006">
        <property type="entry name" value="Succ_Dhase_FrdA_Gneg"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01816">
        <property type="entry name" value="sdhA_forward"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01812">
        <property type="entry name" value="sdhA_frdA_Gneg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE 2 FLAVOPROTEIN SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632:SF51">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000171">
        <property type="entry name" value="SDHA_APRA_LASPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00504">
        <property type="entry name" value="FRD_SDH_FAD_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 20" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8 18 20" id="KW-0274">FAD</keyword>
    <keyword evidence="6 18 20" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11 20" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 20" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 20" id="KW-0813">Transport</keyword>
    <keyword evidence="5 20" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="FAD-dependent oxidoreductase 2 FAD-binding" evidence="21">
        <location>
            <begin position="50"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarate reductase/succinate dehydrogenase flavoprotein-like C-terminal" evidence="22">
        <location>
            <begin position="500"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="328"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="55"/>
            <end position="60"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="78"/>
            <end position="93"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="395"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="428"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="439"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="444"/>
            <end position="445"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Tele-8alpha-FAD histidine" evidence="19">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00059077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU362051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="CAD8156604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="636" mass="69714" checksum="54CFB1B929556ECE" modified="2022-10-12" version="1">MFRITTQALYNQQATKLVNRFCVVSKNKPGVGPSQGHLTDKYTVIDHTYDAVVVGAGGAGLRAAFGLVELGFKTACISKLFPTRSHTVAAQGGINAALGNMTEDDWRWHAYDTIKGSDWLGDQDAITYMCKEAPKAVYELESYGLPFSRTPEGKIYQRAFGGQSLKFGTGGQAYRCCAVADRTGHAMLHTLFGRALGYDCIFFVEYFALDLMMDDQGACRGVVCMSMADGSIHRIRAGYTVIATGGYGRAFQSCTSAHTCTGDGGGMTIRAGLPMEDLEFVQFHPTGIYGSGCLMTEGCRGEGGILRNSLGERFMERYAPTAKDLASRDVVSRAMTKEILEGRGVGPEKDHIYLHLNHLPAELLHERLPGISEAAKIFAGVDVTKEPAPVLPTVHYNMGGVPTNFKTEVLNQVGGKDQVVPGLLAAGEAACASVHGANRLGANSLLDIVVFGRQAANLVGEKWKPGQKQPDLPKNAGEAAIARIDRLRHHEGSQTVAQVRKDLQRTMQKHAAVFRIEKTLQEGVEKVKEIYSRKDDIRIKDKGLVWNSDLIEGLELDNLLLQGKMTIEGALNRKESRGAHARDDFPDRDDKNWMKHTLARIKDTKSGDIELTYRDVITKTQDPIEFDTVPPKKRVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TXF1</accession>
    <name>A0A8S1TXF1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dynein regulatory complex protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0290162</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DRC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TXF1"/>
    <dbReference type="OrthoDB" id="1677536at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005858">
        <property type="term" value="C:axonemal dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070286">
        <property type="term" value="P:axonemal dynein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060285">
        <property type="term" value="P:cilium-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003352">
        <property type="term" value="P:regulation of cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039505">
        <property type="entry name" value="DRC1/2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039750">
        <property type="entry name" value="DRC1/DRC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029440">
        <property type="entry name" value="DRC1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21625:SF1">
        <property type="entry name" value="DYNEIN REGULATORY COMPLEX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21625">
        <property type="entry name" value="NYD-SP28 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14772">
        <property type="entry name" value="NYD-SP28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14775">
        <property type="entry name" value="NYD-SP28_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein regulatory complex protein 1/2 N-terminal" evidence="5">
        <location>
            <begin position="36"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein regulatory complex protein 1 C-terminal" evidence="6">
        <location>
            <begin position="554"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="34"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="156"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="271"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="591"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="34"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8156774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="642" mass="77227" checksum="62996A9E5B0AAC11" modified="2022-10-12" version="1">MDSKKQAKGSEQVQKSRGNIDMLIRTKKEELYTIKQKNDQKQKETRAQEEFKRAERANKIEDESKKANSKNAELEQEWCNLQEKDECEELNKLINQQKDKFGQIMHAKDELIKQFLDELNKKDDDFGKMIKEQAIDIQTLIQKMRSQFFQLRECIHEDLESIENEYIEERANLLNNYQTEIRNLFDKRTEKEKSFVDERERKEEEYTKDIEKLRIQGLKSYAELKISMETEIQNLEKCYEDMKALYQLNTEKLDYNLKVLKEKQISHTHTHDDLKRKDQNLNNRLRSLIKDYNEFDTKFKQTNKELTQEYKRITRQFKELQRKFKHFDKADQKAYADIHSMNELEVRKLKAKILKCDKTIHIQQLGVDWEEPQDQKETQEKSQIMAEEEVQLPLSEEKLQEIVEILIEEVDFLIDDKMRETLEKAEQQEISSVKLGVIKKCLNIDSIDEMLIFIDELIKNCELKVQEEQPQQEAVTEAIKKVQIEQPQTVPDEAPKINYETFILNQNSIINFLMHWMKTNEQRKKQIEKMSSRRAAKQETERQKKERIAKEGKKYWEKLTQVLPEKTFRVWRILDKQLSKYYELLLKRSKLVEETGQIHNHNEELKNLLNQYLQINHELKIPPTRFLKLEQQQDNVKLQNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TYN8</accession>
    <name>A0A8S1TYN8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0280003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8155846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TYN8"/>
    <dbReference type="OrthoDB" id="303689at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="150"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8155846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="346" mass="39607" checksum="A49C0B0123166E7D" modified="2022-10-12" version="1">MRIKQVKRRDSKSGNTRFSALNIQVKCQEFQEDQGYASSLGETDHTNYQQNQSHYTITQSSINYPSKILVGQKLSLRPSTLENVQGLEPQESLKEQQFREIDQIFIRAGNPKATTSTAGFGYKEQTSQKSPQTENVNNTIDEQQIEDSSNESSSFSDDQVSGTSRQTLDQKLRPMKQSNNRSEKYSQYRSGVSSISMNNTPKHSQLSILNTFTLKSDMNNNFQQSGGTNHGSQKIISPLHLREKEDRQNTLTIPGGNNTLQVPSKKSLINPISQLNYEMSPFVAFPQHHPAIYNELQWSPIEIDKYEIYKIFYPKYNIDVVLESYHKENAQKQKSNIKELFKLNTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U112</accession>
    <name>A0A8S1U112_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0310131</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U112"/>
    <dbReference type="OrthoDB" id="660555at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051092">
        <property type="entry name" value="FYVE_RhoGEF_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673">
        <property type="entry name" value="FACIOGENITAL DYSPLASIA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673:SF159">
        <property type="entry name" value="LD03170P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="1">
        <location>
            <begin position="76"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8158044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="543" mass="64218" checksum="F156B76287129A18" modified="2022-10-12" version="1">MINQKIGNQQNIKIQDCQKADINPDQQTTKNLVTNGFKLYDNLQPQMIKKICLMQAYCRMIIQKKKYKCMKAWAIKRGKVYEELIQTEVSYLADLKIVLEKVVKNVKEMKLLSDIEIQKAFSNISQIYDLNKAFLRELQKKLDIYNNNVCVGHIFLKYIPFFKMYFQYLQEFKIDQISILRSQYQEFDNFLNNLEEACVFKGGDLNSFLIKPVQRLPRYSLLITSVVKYTWKDHPDYQDLQKTLNQFASVTQQIDLLIGNILRNQALFELQYKFFNLINVQIVESTRQFILTESLYMINQGQEEQINLYLCTDLIVLTKMSEENQKYIKEGLITYSFLDLQSYYKNEQVPSLNFTLVAGEQQMMFICQDQEQKIKLLQEFDSLFANLKNNASQRSVQCKKKTQIYSPIKVQIVSITRGQELLKSYAQYNIIVINQSQSYAIQTRHKVLLKLQKKLEIEFQQESKSEAGSNILFQNWIKNRLNKEQNDGRKVIVQEFLETLLNSHLVKTRPEYFLRKLKLPQDFYIKHDMFQSNNNSLAQSKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U863</accession>
    <name>A0A8S1U863_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0360173</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U863"/>
    <dbReference type="OrthoDB" id="299694at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8161411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="441" mass="51680" checksum="68E6CB912812B325" modified="2022-10-12" version="1">MNQSIFLFKKEKYREALRKQRNENEFRISRFLPNFCQKGKISSILFSFHTSIEQSSKMILNNDELMMKVYKNQQNKNALLLLSNISASESSNSVDRLIKEQYLHLKFLEIIQSKQSNIYQLQYACEGLTNLLNSKINIQQELTDLLNQDLITSIIKLREQIDENQLIDKDSMLYLCLSILIKIQSYQLQNYVSKPQLIDLFIMNINWFEKNIQVSCENIILICSDIEGLVDLIDDQKINSIIDCAINSLQNRQFDITYWILTALNTLVDFSYCDQKILGIVKNSKIITLLKIIFERIQEQQMFLIEVGLEFIYKTLQFQSLEIRFCFLKFLKDIQNLIEFKVQSSLLVLCSFILVILCEQLDQEESGNIISLQFEILLHLNEQQLTDEVIETLINIWNLLDQNIYKLIRKQKVLLEILAESKNESVSQLAINMMAIQNSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UAP9</accession>
    <name>A0A8S1UAP9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0360157</name>
        <name evidence="6" type="ORF">PPENT_87.1.T0360158</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lin-54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UAP9"/>
    <dbReference type="OrthoDB" id="298259at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005172">
        <property type="entry name" value="CRC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028307">
        <property type="entry name" value="Lin-54_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446:SF34">
        <property type="entry name" value="PROTEIN LIN-54 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446">
        <property type="entry name" value="TESMIN/TSO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03638">
        <property type="entry name" value="TCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51634">
        <property type="entry name" value="CRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRC" evidence="4">
        <location>
            <begin position="14"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8161384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8161385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="212" mass="24508" checksum="9FD045E098FE3DC3" modified="2022-10-12" version="1">MSEEESDQVFEEGPSKKCNCKNSKCIKLYCECYQNKVFCDNNCHCHNCFNNSSNAKQRLKAMQYTLEKNPSAFQPKITTSDSKPDPLNFGKHNKGCQCKKSGCMKKYCECFQAKVPCSDQCKCIECRNYGFLTKEHFIRATRGDEDDFLYQLLLTKDQDHLQSVLSYKKPLFEQKEIEDVSNQIKKIISNNEQSQIDPILDVFIKLLKNASD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UCJ7</accession>
    <name>A0A8S1UCJ7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0360197</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UCJ7"/>
    <dbReference type="OrthoDB" id="292811at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398:SF0">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8161459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="584" mass="68531" checksum="014F3DA1477F22FF" modified="2022-10-12" version="1">MQKNKIFLKGIKQFDLFAQPLQLLVDKKEYHQTLFGSILTVLLLALFSNLLYQKLVTLFDRSNPTSLSSEIYHSQPEQYSLMPSNFTMTFAFQNSSYNTYIDESIYVVSAYLVKKSAKLQDGYKIDVYDKQELPLVKCNQQLIQQQELKEYFSHLDLPTNYCIDWNKIPNIQIQGTFDAPKFEYIIMQFNTCTEQNKKSQPCKSQKEIEQALTQNYFSFQISSQTTNLKNPNSPYQPKGQDLFTTISSNIYKEISIYLEPLTTITDVGLIQTKLEYEKTLRYGRHTEMLDLSQSDLIMNVVIRLDTTEYVDYRTYPKIQEILAELGGLWQVLFSLFYIISKPINKISLLLQLINSLYEFKDKQQYEDNFQQKDSSLIKNEKSGSPLQRQLEQQVQICQDQIVTNKTLSIKENICNKQGRKDRSYKTILQTYVQYLQKALFRKNKNIKFGYINAFQALRCIVTKDDENILQFRQAHKEICNQLNILNILKRLQDVEKLKHIIFNDNQRILFDKFNNIPQKDQQDELKLYSDQQIKQQGITQDVILQILSDQNNEINQKLIASLDVTVSNLLQKCKQDNNDNLEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UFP8</accession>
    <name>A0A8S1UFP8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dynein light chain roadblock</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0390113</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
            <location evidence="1 10">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the GAMAD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UFP8"/>
    <dbReference type="OrthoDB" id="299194at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.30:FF:000009">
        <property type="entry name" value="Dynein light chain roadblock"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016561">
        <property type="entry name" value="DYNLRB1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004942">
        <property type="entry name" value="Roadblock/LAMTOR2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10779">
        <property type="entry name" value="DYNEIN LIGHT CHAIN ROADBLOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03259">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009998">
        <property type="entry name" value="DLC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00960">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6 10" id="KW-0243">Dynein</keyword>
    <keyword evidence="5 10" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Roadblock/LAMTOR2" evidence="11">
        <location>
            <begin position="6"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR009998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8163224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="107" mass="11936" checksum="98702D429FC5AAF0" modified="2022-10-12" version="1">MSDVDIEDTINRIKNHKSVQGIVIVNSEGAITRTTYLNEKKDEGDTIAKSIPILAQKARSLVRDLDPTNDLAFLRIKTKLNEILIAPDKDLLLIVIQGPKKQGEDDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UFV6</accession>
    <name>A0A8S1UFV6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0380123</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UFV6"/>
    <dbReference type="OrthoDB" id="286409at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07982">
        <property type="entry name" value="HFD_TAF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003923">
        <property type="entry name" value="TAF10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03540">
        <property type="entry name" value="TAF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8162562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="136" mass="15956" checksum="E601B2D2430A5CE9" modified="2022-10-12" version="1">MNQQQSDIPPSKKTQFTGSIDNLIYQTISKKPNMSEFLIQEILEASGFQTNDQNCYKLIAHLTDKWMIEMTDSMVQKLIQDRMRKKEKDPDEKLQLTTNDIVNELDQRGLKLHNPFQYPEIDANNLQKVKNDIQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UG93</accession>
    <name>A0A8S1UG93_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0380240</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UG93"/>
    <dbReference type="OrthoDB" id="288968at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05578">
        <property type="entry name" value="STKc_Yank1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000454">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000654">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF374">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="118"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="10">
        <location>
            <begin position="377"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8162789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="453" mass="52920" checksum="44100DC76B7F86C7" modified="2022-10-12" version="1">MKSLNNRFPHSRQDSGQMNITRSQLGQAQQVTNVLNNRQNEQNIDRSPAPMVHGLHKLAVPSSQQSSANHSKKSSISKQQQPKQISFLNYLILKDKSQIAQEEEKENLNEPVVQKNMFNFQFVIGIGGFGKVWKVEYKKNGQIYAMKEMSKALIIAKKSVNSVMNERNILSNLKHPFLVNIYYAFQDRENLFLVLDYMQGGDLRYHIGKMRRFTEDQTRFFMACIFLGLEYMHSKNSLHRDIKPENLVLDKYGYVRITDLGIARNLRPDNSQDTSGTPGYMAPEVMCRQNHSYAVDYFALGVIGYEFMLGKRPYTGRSRKEIRDQILAKQVQIKRSEIPDNWSLESADFINRLIQRKPANRLGFNGPQELRQHSWFKNFPWSKLMNKELKSPYIPNQNEDNFDARQISMEDEENNELIQQHSIMLRRNSIQSQFSGYEMDNFNEKQNTLFNNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UKU0</accession>
    <name>A0A8S1UKU0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine/threonine specific protein phosphatases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0410210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UKU0"/>
    <dbReference type="OrthoDB" id="309289at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000161">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000205">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46422">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE BSL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46422:SF11">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="1">
        <location>
            <begin position="472"/>
            <end position="771"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8164783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="824" mass="94228" checksum="0FE86E5591A64506" modified="2022-10-12" version="1">MESVNFGGDNPQPRFGHTICVIAPNKIALFGGAVGDTGRYVITGDVYIGDMTSKKWKRIEASGSVPTNRAAHQSLAIELNQMIIFGGAVGGGGLADDNLYVFELRDDTGTWVTVPVIGTTPGRRYGHTMVLIKPHLIVFGGNTGQEPVNDVWSFNLEKSPYSWQKLECSSEQPNVRVYHSAALCTTGSANGMMVAFGGRTNDQGALNDTWGLRKHRDGRWDWVRAPYRNQTEQPLQRYQHSTLFLGTLMLVIGGRSNNVGETLPFEIYDTETSDWYKFQAIQRFRHSSWLIDQFLYLHGGFDSDQPNIPTEGILRLNLNTRFAQTPQLLRQMNTLKTDQSFTQSFNPRPPTNQAQTQDQFRRTNQQQAMVKNQNNNQQIRVSSATNKNIRLANQAIVAMTYGPEEDITNQVKKVPIDKLQDEHKKLGAGFQDPNSQNKSQFLDQLCQPFVQNLLIPKDYQSIPPNSNLLTGIRKDMIIKLCDEVQRILDKEPIVLKLRRPIKIYGNLNGQFLDLMRFFDHFKAPYDNLYNGDIDSQDYLFLGDYVDRGTRSLEIVLLLFTLKLKYADQIHLLRGHHEDAKINKIYGFADECFIKFAEDIMDPNSIYQRINRVFQYLPLAAVIEDKILCVHGGIGQTMRTVDEIELIQRPLEIVHDPKTYQQKIALELLWSDPCLSEDELENQPNTERDIFQNRQIIRFGTNRVSKFLQENNLNIIIRSHEPTLEGFERQNNNVITVFSCPDYGTNSQNNKGSMLTISKRGEIIPKVILPVNSTSESRWIDLEEPIQRKKGFAKYVVIDNDEQQYRKRQFTPLRSKKSSSQKQFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UM57</accession>
    <name>A0A8S1UM57_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0440093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UM57"/>
    <dbReference type="OrthoDB" id="301300at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="31"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="196"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8166328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="243" mass="29453" checksum="BE7282433F0C26DB" modified="2022-10-12" version="1">MSRTNLGSPSYYFSSSAPKLENFQQSPKKEFFDASRQLEKAKRRVQLLKLENEIREKKAQIQQQKNEQLTRIREDHEQFREFKQMHKEQVNMQIQNLVSRTRNLKRQQSQEKIYIQQNFLQMKQQEVKNIKEQSQKNEQLIRQNLDNLKQQKYEQKQQIITQEQLAQQSINNFHKQRYLKHQQDHYHSKSQHMIEAENKLSIIEELRKQEAQLLQKLETTSKMNTSLVARQMSTTTPKTEFYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UNG4</accession>
    <name>A0A8S1UNG4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphatidylcholine transfer protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">START domain-containing protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">StAR-related lipid transfer protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPENT_87.1.T0400170</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="8">Interacts with ACOT13/THEM2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8163996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UNG4"/>
    <dbReference type="OrthoDB" id="5403181at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00177">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000017">
        <property type="entry name" value="Phosphatidylcholine transfer protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051213">
        <property type="entry name" value="START_lipid_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308">
        <property type="entry name" value="PHOSPHATIDYLCHOLINE TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308:SF14">
        <property type="entry name" value="START DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00234">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="74"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="START" evidence="14">
        <location>
            <begin position="484"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="322"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="371"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="322"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="387"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00077188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00079049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8163996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="684" mass="79694" checksum="72E6AA2AC14DCC10" modified="2022-10-12" version="1">MINMKLKNPKSLTSLVNLYLKNIHNPMELKDLINQLLSDATFLPIYGLTDQQKLEHKTIFTNIQLGNNQNLGTIIGMTVLGVIGFLALGFTFLGAIGGGAIGIILGRYLGRKISKKIATKGINLTEFDIFIIRVKCLLKWGKLIMIKYTNNINLQRFCVQRILTEIKPLLHYKYFDEKFQKESHTLLVKIREWLSKEQSIYALCLSYKLTKEYIQIVEQKIIYSKFSNYEISYVLNETVSDVLIPFLTLFQDAKQYEKNDVIRLLIQKIKQLQVNKIVLKCQQSYFDQQLSIEFIKMFAAKLNQKVKVEDLEKFLQIQNYRQQQQNREQNSPSKSQQRRKSQGDMVSINEDIDQEFQAQVPLIMKNQVRARAKSYDDKNDQQFTSKQQSQLIIQQNQQQQQQQQQPLQQSDQIIFDNTEKRGSQCIPENLIQQSQLQQILCLQMEHSQQQSQIHRVQQQAQDNQINSDRTYQLSAELEEKFKLLVDLIEEPIDGWEIILKKENLIIYKTFKPGNPAVFVKGHSDLIGITIDVLLKAIHNEKYRQKWDKILLSFQVLERESAIVDIIYYYVKSPPCVDNREFCQKRILKYDFPKPGQTCLLYFSVEHSKAPKFKGHIRAETLISGYILESIPGGSRLYFCSNNDVKGDIPKSLVNYVASRAPISWINSLRKGCEDYNKIMQQIQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UR29</accession>
    <name>A0A8S1UR29_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0460216</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8167075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UR29"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8167075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="85" mass="10190" checksum="76008FC19717E7FF" modified="2022-10-12" version="1">MLSKLSIIKGRGLRNLSLRKRNFAKIMEQNEEQEIKKLDIKQESQKEIQELKAYTQIEHRNDQGLKYHLSCMHSHKNSQHLQNYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UVL2</accession>
    <name>A0A8S1UVL2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0490117</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UVL2"/>
    <dbReference type="OrthoDB" id="284883at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8168454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="122" mass="14960" checksum="3CBA0435CDE60943" modified="2022-10-12" version="1">MNFIRKRLGLLPQTTAIMEIYQSEQSNEVIYLYRRILKQISITYERRLERMQKLKEAQWIFRECKDEKEQEQIKYLKNQGYLVLKMLEQNQVLPSFYPNPLDLVEPEVHNQFGKQLYNRVKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UY89</accession>
    <name>A0A8S1UY89_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0510189</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000051">
        <property type="protein sequence ID" value="CAD8169671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UY89"/>
    <dbReference type="OrthoDB" id="10625861at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8169671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="510" mass="60237" checksum="6566AB672F495F87" modified="2022-10-12" version="1">MKTILRLPKQTSDSFMEMLLQKMEIMNKISIQRVYKKALDRGFNLQSNIQDLIDHLDRIIQKGKSKLLNTLPKNLEYLFKFGQCYCTIHNNQRITFKHILQDHLGLTNQIFLEQLISNGLNDYVKDRIMNKIEQLQKIILYREENLNIYISIIPLKNSEIIIIIGFSFDQTYIPQNYFDVNLIVCIKFNSHRVNCNNILLHFRLENAKAAFCSFQELLYSINLINNIPVCIYDEFEMKIYISKQILMLFNMIVNKVVSSEDQIIMLSLYFNIDLNFQYFYRRINISLNQENCFGLDISNSNYILISKIIIQFLECKISSLKTKQYISTNQIKSKIDSQNVGDIYLFSNYYILRIGITIEEANRLVGSLKSLYTGLVLKEYYLPKFKSASFKSEYLLGFVQMRVFTCQAKNIKIAYLIKEATKIDLYRELHQLVGKEIDLGLTSQKLPDREWLINVLYFVSPNHSYFKQLTTNQLKGISQQDIQHLYIYIHQRVKQINVQIKKTQYFQNSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UYN9</accession>
    <name>A0A8S1UYN9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0500268</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8169303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UYN9"/>
    <dbReference type="OrthoDB" id="1872379at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050498">
        <property type="entry name" value="Ycf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE SPINDLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="60"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="232"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8169303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="306" mass="36125" checksum="D7EB89F384D28CB2" modified="2022-10-12" version="1">MEALKDCEICIQISPKYSLAYMRIGQEYSLQLRCVKNAIENKDDELQYYNQVIEIDNKCILAYCNRGNLYKNQGNKDQALLDYNKAIELDTQYAFAYMSRGSQEFFQIKQEIKRRRFQILTRLLNQTLMMLVFIINEDFYLLKLEVLNRCLQTLTRQQNQILIVLIFISIEEIFIKTQIMNNMYSQIMIKRLNQIPIIKAYNNRGVKICTIGNTNIVLINYNKSIELDSNDAYTYNNRGMLYQNTGKKMEALEDYNKAIQLDPKIAQFRFLGYKYQAIIDHDKAIQLDPKKLLIISTEVIKMLNQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UZS2</accession>
    <name>A0A8S1UZS2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Aurora kinase</fullName>
            <ecNumber evidence="11">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T0510216</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="11"/>
    <dbReference type="EMBL" id="CAJJDO010000051">
        <property type="protein sequence ID" value="CAD8169723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UZS2"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14007">
        <property type="entry name" value="STKc_Aurora"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000235">
        <property type="entry name" value="Serine/threonine-protein kinase ark1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001072">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="152"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="46"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="277"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="8">
        <location>
            <position position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8169723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="418" mass="48804" checksum="C9B8C8A9101FF08B" modified="2022-10-12" version="1">MNYPYQPYRPYSRLPNSSMHNSNIMSYVDSVLSKSRNNYASVQLEPQNKYQTQQTNSIKTDTDLKNIGNSPLILPQKSMVIQPTLKSKQEYQSPKKVESQSFQSDIPPVIPLIPLMSDVREQYTKKQVSTPVSQTNFPMKQPEKPKEKYKNFTIIKKLGDGQYSEVFLAKHTQTGFLVALKVIQKSQIIKENMQAQLAWEIKIQYLLEHPNITKLYTFFQTPTEIVLVLEYCSHGQLNTLQQMQPMKKFQERIAAQYVQQITFALMYIHNQDVIHRDIKPDNILLSFGLVKLADFSFCVYSPDEDRQTQCGTIIYASPQILEGETYDKKSDIWGLGVLTYELCFGKPPWKENQQELMKTACFMIPYTASRDLRDFIENLMKRLSRDRYTAQQAYNHAWLQRIAQIMPYYIKNNETLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V165</accession>
    <name>A0A8S1V165_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein L19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0490133</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V165"/>
    <dbReference type="OrthoDB" id="432645at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001857">
        <property type="entry name" value="Ribosomal_bL19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15680:SF9">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL19M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15680">
        <property type="entry name" value="RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01245">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8168476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="263" mass="31061" checksum="96B12C17C6A80A73" modified="2022-10-12" version="1">MIGFYRSLVNTIPRFNIVYGYVPKRSPFDKTHYIENYIPRQIPYPKSRRPRLPQWDDEQTVWPIIPPSPIVTRKKLLQQLEKEEYERLQIIKPMTFPDFRVGDVIQVKWIHNMSEPTMNTYQGLCVGKRRSNSLDASFKFIFRYCGVDVFMNVKQNSPYLKEVTILKKGTGNIRNKVALFTKRLAKQQFITPIMKKGKPVRRKDDPIRKRSSRSRTSISMLKSIEDPLLVEKPVEKKAKKVYKKKQAEQETIVEPNENTNKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V5R5</accession>
    <name>A0A8S1V5R5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0550195</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V5R5"/>
    <dbReference type="OrthoDB" id="303921at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="181"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="24"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="189"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="241"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8171753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="568" mass="68540" checksum="5FAC7DC4DDA7B9E5" modified="2022-10-12" version="1">MSKTPNRIILPELENKSFRDTKQIQDLRRKLEQQQDTIKELVNLVKESKTESYKPSFNQTIIQPKPEKDEVTLQLLEEVKTLRKQINNIEQGPKQSVQHPIIIPQYLPGPPQQQQQPMISPYMFMNPYFPMPPPFMYPYPQQSNNDDPYKKIVLELLQKNNKINRDLNYKLRDSYSSEYTDDLYSRNNSRQHRHHSNVDRRKHHPNDRYNQRKNRDKKQIKDRNQNKIRDQDDSDHHRTISRTSMNDSQMSYNSNQNGKNKKKNNQKIFTEEEKLKLRRKLIGLFWYIRIGLVLRKYLKRVWLQRRQEYYETEAQQLIDKFDHEFNYKEVFILFVVECNKNKYFTKNWSIFDNKEVELKSKLIFSIITALFKKIPFMTKNSMTDEHRQFIKKISTSGGYLLPGHPQFVTDRVELRPNVTIGQINADVSKLIQMDYVYIQILVQRVILIYEWYSQFSKIPNYKEAMKIFVTLLHQLFIDHFINLPTFENPDQIFNQQQIVYCDFNQQNYIDVAISIDSKHQKYQPTKNCCILGLASYEEMRPLYEQPGYKDIQIQFKEYCEYFYQSLNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V6Q5</accession>
    <name>A0A8S1V6Q5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pyruvate dehydrogenase E1 component subunit alpha</fullName>
            <ecNumber evidence="4">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0590032</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="4">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + pyruvate + H(+) = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDO010000059">
        <property type="protein sequence ID" value="CAD8173278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V6Q5"/>
    <dbReference type="OrthoDB" id="10256198at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02000">
        <property type="entry name" value="TPP_E1_PDC_ADC_BCADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000013">
        <property type="entry name" value="Pyruvate dehydrogenase E1 component subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050642">
        <property type="entry name" value="PDH_E1_Alpha_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017597">
        <property type="entry name" value="Pyrv_DH_E1_asu_subgrp-y"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03182">
        <property type="entry name" value="PDH_E1_alph_y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516:SF60">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT BACTERIAL AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="5">
        <location>
            <begin position="47"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8173278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="372" mass="42022" checksum="92D2A020EAA8CAE1" modified="2022-10-12" version="1">MIFRISRSCFSSISVKLPPFKVHHLELDQLPQTTTTTSAELLAYYKSMQLQRRMEIACDNLYKQRLIRGFLHLADGQESIYEGLHAALTFDDCVITAYRDHCIALLRGDTPHQIIAEMMAKQTGSTKGKGGSMHYYKKATNFYGGHGIVGAQIPLGTGLAFAQKYNKKPNVTLIMFGDGAANQGQLYEAANMAQLWHLPAIYFIENNLFGMGTSIDRASANTKFYTRGDVIPGIQIDGNNVFQVRETLKFAKKHCLEKGPIFIEAMTYRYHGHSMSDPGVTYRTREEVQQQRKTRDCINYVKNIILENKVADEHQLEEIDNTAQNEIDIAVEQAKVDPVPPSTELTTDVYVDNQNHFIRGILYKDSILPKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V7C0</accession>
    <name>A0A8S1V7C0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0580065</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000058">
        <property type="protein sequence ID" value="CAD8172814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V7C0"/>
    <dbReference type="OrthoDB" id="2158884at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07831">
        <property type="entry name" value="STKc_MOK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000271">
        <property type="entry name" value="MAPK/MAK/MRK overlapping kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000773">
        <property type="entry name" value="MOK protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="4"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="309"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="310"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8172814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="446" mass="52125" checksum="EA1D90EBBB1EBA43" modified="2022-10-12" version="1">MNQYKLVGKKGEGTFSEVIKSQSFKTGNYVAIKCMKNKFTSIEQVNHLREIQALRKLSPHEHIIKLIEVLYDEPTGRLALVFELMEQNLYEHIKGRRQPLNPQKVKSFMYQLLKSIDHMHRNGIFHRDIKPENILLNSDHLKLADFGSCKGIYSKHPYTEYISTRWYRAPECLLTDGYYDLKMDLWGVGCVMFEIIALFPLFPGTNELDQVNKIHNILGTPNPKIFDRFRKQATHMEINFPNKHGSGIDKLLQGQSKECIDLIKMLLVYDPEERITAQAALKHEYFRELYEADATQKSFQHTLQQIRISNHREQNDNSLERSQRIEENKQMHQPNFKKTQNYYAKSQKKSGLPSLQFDLKVESIYKNTQHHDSDDDLDKQSSNKQMMLPEIKKKKKYDPKVIYGKQQFQSSQAYQFNSKAGKKMAHGLQADYIVYGKKAVNQQILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V7P6</accession>
    <name>A0A8S1V7P6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4200 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0530256</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V7P6"/>
    <dbReference type="OrthoDB" id="10264063at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051147">
        <property type="entry name" value="CFAP_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025252">
        <property type="entry name" value="DUF4200"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21683:SF3">
        <property type="entry name" value="CILIA AND FLAGELLA ASSOCIATED PROTEIN 100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21683">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 42 LIKE-2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13863">
        <property type="entry name" value="DUF4200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4200" evidence="4">
        <location>
            <begin position="127"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="201"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="319"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="447"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8170806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="557" mass="66877" checksum="9CE130F0BE03B564" modified="2022-10-12" version="1">MIDNPFEFERDSKKFTNSLEHDRNAIEEEDDKQQQTRLTQLYIGKSAGRYGLLRKLEEQTEKLAVERDLVFSQDLKTKSYNLKPDPIQCHLKQFNPRMLEKNYQDSLQKAYKIKSILNEKKETLQDFVEKKREICLTNLNILTKKEETNRLEDFIKNEQESLQARKLYFKNDCELVKRFMSEVKSQADQAAILADKEMRRKEEVKVEVARILAQIDRLKLLKSKLQDEYDKLDKYRQFLEKIKQHYKTKFSELEKDIILPQSISNSKKTLFLTGVNVLESEIRSKEQIESLQFEYKQNKMADIVIEILDAIEEGNLRNMQNQRDSEEEIEQKRREAQRLKEIFVIHQKENEQRLKILEKQFVMHQQYQQEQKKQEKVDQNKLEFAEGENLDMDQIGKRIIEIYADVSKKDQDPLIKKLGIDSKLLKITLNQLEKIVLELSEYKLQFIMKSKQDYLDAEKKINQKKKEQRQTIQKDEEKKKQIIERRRKKEEQMANFYRGRKDMRRNYPEEKVIVEQVVEDNGENDDEKYLQENYELVYVPSQKTDNISSSQQVQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VHY5</accession>
    <name>A0A8S1VHY5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0610042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VHY5"/>
    <dbReference type="OrthoDB" id="307031at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8174346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="127" mass="15371" checksum="5DA09DF72B7B65B5" modified="2022-10-12" version="1">MQNKRHSQIFKLHRNLLIFNKIQDASSIVVESSRNQQFRNRKNKQYPQLHLIPFGNQREIKTDLDSETDRYLQPQKTKMNRRCSHSIRRNQIEYNFNTSVINYSFQKSKKDQACQTYFLDEFVNVDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VJX8</accession>
    <name>A0A8S1VJX8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0670018</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000067">
        <property type="protein sequence ID" value="CAD8177091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VJX8"/>
    <dbReference type="OrthoDB" id="282867at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8177091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="256" mass="29292" checksum="CEF591C5997C7444" modified="2022-10-12" version="1">MEQQHKGNTEPGEMNSSSQQFYSTNTLFLNNIGLSTNISSSTLRHTSPQISFPKANRFHTQSQLQLPTKLELPSQLGKRFTQQGFGNKIVFQYEWQKVNAKELPSPDRYQVRQEPGSDKLKKSFGLSWDAYSKTYLPYNKHLSPEVARYLPGPGEYTVRQDLGQHRYQFQLKGKGKMFNDQRENGVPGPEAYQPQICLTVPNRFSKISLGIGEKKNPFQSFSFAPGPGRYETNSAFDKASKKREFINKPGERRPFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VLB1</accession>
    <name>A0A8S1VLB1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">cathepsin L</fullName>
            <ecNumber evidence="10">3.4.22.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPENT_87.1.T0670139</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="11">May be involved in extracellular digestion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Specificity close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity.</text>
            <dbReference type="EC" id="3.4.22.15"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.15" evidence="10"/>
    <dbReference type="EMBL" id="CAJJDO010000067">
        <property type="protein sequence ID" value="CAD8177333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VLB1"/>
    <dbReference type="OrthoDB" id="190265at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000104">
        <property type="entry name" value="Cathepsin L 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035802643" description="cathepsin L" evidence="12">
        <location>
            <begin position="21"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="14">
        <location>
            <begin position="29"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="13">
        <location>
            <begin position="118"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8177333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="312" mass="35490" checksum="4C91575A59F42747" modified="2022-10-12" version="1">MKSALIILIGLLSAFTYLNQEENDGHIEFQSWKREYGKSYTGQQEVFRFFNFQINLNKVNKHNSDKNKTYKMRMNQFSDLSEEEFSLLYLTHYISDEFIQQKQITDEQESSIKKNDKTKTSVDWRKITQVKDQGQCAGCWAFGAVGAAEAWFYVKNNTSVVLSEQQLIDCDTQSFGCNGGYQNLALKYIANHGLNDARVYPYTQKQSVTCLYNSGPYKTNGAQSVSSNNVKQFLTEYPLVVVVDASNWQFYGGGVFKDCSRSVNHAVLAVGFDENDNWFIKNSWGTSWGEKGFMTLAPGNTCGITSYAYRAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VML6</accession>
    <name>A0A8S1VML6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0680165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000068">
        <property type="protein sequence ID" value="CAD8177901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VML6"/>
    <dbReference type="OrthoDB" id="309623at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="170"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8177901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="405" mass="47000" checksum="85FA1B079BA0DE16" modified="2022-10-12" version="1">MKEQGRYSKLSEEEAAAQFELRKQQIMGNLLEQYENSQKNKKASQISKSQINQQKFIVFNQKKPLLKNTQNESNQLKLVKEESPQQNSTPSPESEDEQIQQIVEPIPIAQPPSIIKNDSNHLKKILLKDIGNIDLEIERLTRETKDFEIDKQANKSQFSYQSSSINNGASKNNPKQNSSQQQQQKKLYNEQRQRPSSKESHISESSHIKMVECFKGVMRSQSLKKLPQQQQQQQQQYKFSQQKYGSEKKINQSINKTVTIESENKESTTNQISYSERKAPRSTINRSQTKQQSPFIEKKSTPIKTMLKVQFNSIPIGSNSTKNKSVSYQLQDFHQHKESYNSQVNLPKNQCVESQIEEAVQLYKNLKFLMQSKSNVLQIKKDVNQIPRKTVDFFQHKEIAKPTLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VPS1</accession>
    <name>A0A8S1VPS1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0680015</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000068">
        <property type="protein sequence ID" value="CAD8177652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VPS1"/>
    <dbReference type="OrthoDB" id="2942533at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050498">
        <property type="entry name" value="Ycf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE SPINDLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="43"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="112"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="265"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="299"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="333"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="367"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="401"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="435"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8177652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="483" mass="55983" checksum="E0BBD4283618164E" modified="2022-10-12" version="1">MKQNLTFEEDNYNYLDKNSYQQHVQEINRLTQMINSNMFLDKAELYYKKGMNLFVIKNHEEAVQSFNKATNIDKNHAEAYFQKGQIFRTFLKDPKAAVNELDEAIRINPKYVEAYNCRGYALMDLHKYEDAAESFDKIIRINQENNRICPYSILEQLYFCDGYQGKASALQELGRWEEVLEIYNHLIKQKPNYEHHHHDKAKALLFLNRIEEALDSINKALIIQPDNPLFLFTKGQAVALSRNRKATEEFFNQSLQQKSKFFDSASTYLEYGLAMDILGDKVEAIRNFDLAIQDSPDYYQAYYRKGLILISLGEKMKALDCFNKAIKINPDYIEAYNAKGLMLSELGQNWEAIKCYNKVIKMNPNDAHAYNNKGWFLSQEGDYESGLECINHSLEINPKIAHTHHSKGFALSALGRQKEAIECYNKAIELDNQLICAHSDKGMSYLLLKEYSLAIECFDKALSFDANYTQAQKGKIQAEKLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VXC9</accession>
    <name>A0A8S1VXC9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0740165</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000074">
        <property type="protein sequence ID" value="CAD8180522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VXC9"/>
    <dbReference type="OrthoDB" id="304563at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035768575" evidence="2">
        <location>
            <begin position="21"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="53"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="256"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8180522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="357" mass="40632" checksum="3CEBB553B5F4CF3A" modified="2022-10-12" version="1">MKTILLIALIAVAFTARVQERNLAKITTDLKKSTYGSALLHLVELHSMAGGPVQELIDAIEELINDLEEELEELEFNFQVRTNEHNALVVSLEQDIQDAVIDVNNTQDTLDNLLFPRREQLQVRIEQIQENQEANRKNYDEAVLVREQEHEDFEFQIAELNDATAAVDDALALLQTLTNPSLLQVKRFQNSLKNIEAKIKSRSKMAPMIKALISLASNQNFSDQGIIGQIVDALNEFRNAIVDSINAQTAAEAQAQADHEEYLEQLDDEYAEFQRQINRVNVDLTATNEKIDQMTEFRDQRDADRKQYTAELELENNTYAEETDTYTNLKNEFTRELGISEQALSVVQSADFSNIQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VY35</accession>
    <name>A0A8S1VY35_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0790054</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000079">
        <property type="protein sequence ID" value="CAD8182654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VY35"/>
    <dbReference type="OrthoDB" id="305637at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8182654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="211" mass="24576" checksum="CDCB2ABBCD40B06A" modified="2022-10-12" version="1">MLQKDSELKTVLIKLDDFQKKQDKNKMEFEKNLFQKGLTFSNIYKNNDIVISDNGKIVDIGTNKVGYCMCEKVIPKIGQISFEFEILRISQWCEVGIGFRGMVTQNEYQGDSQGKGSYLLNQSSGTYFHHTNNTLGKAFKFQVNEIIIMEVSVEHKYIKWTKQNSQDMLITQIDTSQNFYLFVNMNNSKVRIKDIYIIMNFQLSVLYIIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W2P1</accession>
    <name>A0A8S1W2P1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycine cleavage system P protein</fullName>
            <ecNumber evidence="5">1.4.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0800137</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="5">The glycine cleavage system catalyzes the degradation of glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[glycine-cleavage complex H protein] + glycine + H(+) = N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[glycine-cleavage complex H protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:24304"/>
            <dbReference type="Rhea" id="RHEA-COMP:10494"/>
            <dbReference type="Rhea" id="RHEA-COMP:10495"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="1.4.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 4 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the GcvP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.4.2" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W2P1"/>
    <dbReference type="OrthoDB" id="6537869at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016594">
        <property type="term" value="F:glycine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004375">
        <property type="term" value="F:glycine dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00613">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000005">
        <property type="entry name" value="Glycine dehydrogenase (decarboxylating), mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000007">
        <property type="entry name" value="Glycine dehydrogenase (decarboxylating), mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000224">
        <property type="entry name" value="Probable glycine dehydrogenase (decarboxylating) subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003437">
        <property type="entry name" value="GcvP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049316">
        <property type="entry name" value="GDC-P_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049315">
        <property type="entry name" value="GDC-P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020581">
        <property type="entry name" value="GDC_P"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00461">
        <property type="entry name" value="gcvP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773:SF1">
        <property type="entry name" value="GLYCINE DEHYDROGENASE (DECARBOXYLATING), MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773">
        <property type="entry name" value="GLYCINE DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21478">
        <property type="entry name" value="GcvP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02347">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 4 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Glycine cleavage system P-protein N-terminal" evidence="6">
        <location>
            <begin position="39"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Glycine cleavage system P-protein N-terminal" evidence="6">
        <location>
            <begin position="476"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="domain" description="Glycine dehydrogenase C-terminal" evidence="7">
        <location>
            <begin position="792"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4">
        <location>
            <position position="726"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR603437-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF21478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8183213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="972" mass="109102" checksum="1A1958D2F2C34439" modified="2022-10-12" version="1">MLRSIRLIQRVANFSRLANPHEVNPKIPDWFKSSDYIEQRFIGSEPQQVKEMLKAVNANSLDELVDKIIPKEIRSEAAFQSPDNFPDAIPESSMVQHLQSLANKNKIYKNYIGQGFYGTHTPYVILRNVLEDPGWYTSYTPYQAEISQGRLEALLNYQTVITELTGMDVSNASLLDEATAAGEAMFLANSWFEKKKFFVDNHVFPQSIEHIKTKAYYLGIDIVVGDAKTYDFKDADQYCGVLVQSPDNMGEVHDWSDLFKHTLKDAKLLKVIGTDLLSLTINKTPKDQGANVAYGNSQRFGVPMGFGGPHAAFFAVEDEFKRKMPGRIIGISKDANGRSAYRMSLQTREQHIRREKATSNICTAQALLANMAGLYATYHGPQGLQKIANRVNCLARSFAKLAKSLGIVVKEGRIFDTVVLHNTENLQEYLHYNAQINVRKIGQDTIFSFDETHTLQDVEDLFNHLQHYTKKKSDFMSVIQKVIPYKSERAPYMQQKVFNTLHSETEMLRYINYLRQKDISLAKSMISLGSCTMKLNPTSFMLPVSFQGFSQLHPFSPLSCTQGYQELTDNVEKWLCDITQMEAVSLMPNSGAQGEYTGLLCIRKYHIMNGQKDRNICLIPISAHGTNPASAVLAGLNVVAVNVIDGYVDLNDLNKKIKENEKSLACMMITYPSTYGVYEDQTKKIIELIHDHGGLVYMDGANMNAQVGYTSPGYLGADVCHLNLHKTFSIPHGGGGPGLGPIAVNKKLAPYLPGREYSLGSVASSLYSSASIIPIPYSYFGQLGRQGAKKCTAMAMLNANYLMMSLKNDYKVLFTGQNGMCAHEFIIDIRPIKQESGITEEDIAKRLMDYGFHAPTMSFPVPGTLMIEPTESESKSELDRFIEAMKNIKLEIEKVKNGQYDKNDNPLKNAPHTQDQVINSGWNHKYSREEAAFPLPFVMQRGKVWPTVSRINNAFGDRNLICQCPSVSDYQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W5L8</accession>
    <name>A0A8S1W5L8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0810120</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000081">
        <property type="protein sequence ID" value="CAD8183675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W5L8"/>
    <dbReference type="OrthoDB" id="292829at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8183675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="310" mass="34882" checksum="17E1E7573EADCD2E" modified="2022-10-12" version="1">MTTPESLNPAPLTGLGDYSYKKVLEDDYSTGKLNLKFKGNTKSSGIANYKGWLDMSKQQSQQETKFQFPYKNYFLQIATREDGAKVHIDFGEVAKLGNNGSVNLFANAKLGSSHLSQAVIRFGGVTQWRSITHHLRFEYNPANAVSTLSRAFWKNKDWVVVAAEDFQVTNGFGVKRLDLIVGYLSQNYDIFFRHLTEAPNKKKEFSQLKNGRLILDFVYRKNKYTFGLETEYNLAKSQLNALVGASTKIEKVDVKARINVTQQRLGLSGKGKLNDRFNWALSTELPLDGSLPNKQGFLPLPIGFTLDTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W5V1</accession>
    <name>A0A8S1W5V1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0820228</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000082">
        <property type="protein sequence ID" value="CAD8184273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W5V1"/>
    <dbReference type="OrthoDB" id="8883818at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="119"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="158"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8184273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="320" mass="38263" checksum="EAEB3EC788510957" modified="2022-10-12" version="1">MKNLNQLIKQKQITQSVLQDLFLTSVHLKERQLIIKFKKFIKNNGVLHLHSINKVQQWLENLKMMIIQRFRSKLKRSQFRCYINWFITGSIDCKIIGSKKIANSIYWVPSQPCDTYSIVWCLFLNQSENLIFSGSSNGSIKVWTLNTDKNQLMFDYQLNKHSKNSGQVSMNEEKTQIVSFSDDKTIIVWKKKANERKFKFKQVFENLKNDNGFSDQYQGSFHIFKQEVEKFVDKNEFQINLRINTYRWIILIFFYLQIFIIKHYKYVNILSYQSQSFNVQNEPNDCRSCKNYGNITEDGNSLIVWNDQTLSFYVYELKYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W6X3</accession>
    <name>A0A8S1W6X3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0770009</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000077">
        <property type="protein sequence ID" value="CAD8181666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W6X3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8181666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="125" mass="15147" checksum="B25E2558F2344C50" modified="2022-10-12" version="1">MIILGFINFLISYKFYKNLIFCKYQNWQLINEKDSHQRKYYQYFIYSGLIRIFKSNFQESIFSSILSLANFNKETTINTTISFFNSFYFGMQLYPFAYFYSYLSYKNLTNHLSVLEKGSKNQSFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W7S5</accession>
    <name>A0A8S1W7S5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0840069</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8184913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W7S5"/>
    <dbReference type="OrthoDB" id="283108at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053299">
        <property type="entry name" value="ASTRA_WD_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44156">
        <property type="entry name" value="SUPERNUMERARY LIMBS, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="161"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8184913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="375" mass="43915" checksum="50E2296035DF08AB" modified="2022-10-12" version="1">MDEEKPGFFEQLRQLYQNREKIESKIVGFHMQKQFKISHLYGNEEQINCMDLIDISENESFIIVCSDAKIKLYSHTTKLDKLFEFVIQFEANEHLDYDGLKNEKQKKFLRHSKQLTTGPVKELIMFCHFGYIHDQLYVFAAGDLGYVYQIEIKENPEYFLLEGHHNVITGLASNNKGVFSSSKDGSIIQWDVTNRQIVQVLRDGDTPEAEVLCVSANQEHIIGAYSDGKLKLWTIQDYKNDSWTYKINKQPTIIFQGPLQDIYPHITQIFLLKQTIVTKHLNGILNYFVFDLQGRTISLRDWEMSNENYWESIDVKFPYLIYTCFNRMQVITLKKNKLTYEAELDVLPSSKLTISKISNRLIVIGRMNQYTILQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W8J5</accession>
    <name>A0A8S1W8J5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0850228</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W8J5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8185701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="86" mass="10318" checksum="82BC645FE90454B7" modified="2022-10-12" version="1">MNIYQPNQSVLNTAFISQAILTEQKIIFLRQSLNKAQRDLNDERFQQNHLQSKREDLISNHQKNHRKLLKQVDEQQQLIKKTQSYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W9D7</accession>
    <name>A0A8S1W9D7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0850082</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W9D7"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002303">
        <property type="entry name" value="Rab_A02 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="173"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="177"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8185433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="225" mass="25369" checksum="CADF519940D2E372" modified="2022-10-12" version="1">MIKEYDYLFKLVIIGNSGVGKSSLLLRFADDQFSESYLTTIGVDFRFRTLPIDGKNVKLQIWDTAGQERFRTITSAYYKGADGIVMVYDVTQGQSFDDIDKFWLHEVESYGEKNVQLLVIGNKNDLDEQKQVETSKAEEYCKSHNMLFMECSAKTADHVNNAFLELSRKLMAKKDASQPQKTTNLTPNASQQSQSRGQTNTNPSQSNKLSANTSNQKKQKDGGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W9Q4</accession>
    <name>A0A8S1W9Q4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0850148</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W9Q4"/>
    <dbReference type="OrthoDB" id="305310at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035722763" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8185553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="420" mass="49328" checksum="2C1D957438423834" modified="2022-10-12" version="1">MLFFILINLIFACEYESTYANITNINSLIPINHIIQQLTQTQTLQDTLELTNITIYTQHINHSYTNIGQITIYLKQSISYNFINNTFQTFGQAILQDVQFLMKLSLNTQTNTFQSNSIQYNLPTQFNFIGLFNYYQYYQSLVTLITAQIPPQLNQLFNAYQFQFQPIFPDYTYTYTNIDDESLIIEFSYLSNNILYQNQDITLTGQLIINNYNNNICTQTQLPNNSNQFQQLEIFSTSIIQSIIQYAYDQNYFNINLSNNWKSDDFQFLIGSFQTILYNLEKISPITPVTGWCNLTQIPVYNPLNITLTYECNFIFSEDQIRLLTLNYELVLNLNFQQVLSQIEIICTSYTFQFNDLIKSFNEKYPPKSQFWSLADLYLKTILNTHVKDHPLFGSGFQTIPRMTPNITINNDYLMIFEND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WB68</accession>
    <name>A0A8S1WB68_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0870162</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000087">
        <property type="protein sequence ID" value="CAD8186611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WB68"/>
    <dbReference type="OrthoDB" id="291221at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8186611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="217" mass="25836" checksum="9208AE3C68F03DB3" modified="2022-10-12" version="1">MGALCPRSNSEVVHVEQNQNEIMIDPQVEIETLINQETNYKLSLSRNFSLNNPTLQTCDQVIQSILSISLSFMVKDFSEQINFLKLQIHKQIQDLINHKIDFQDELLQMLVDYYEFLSYVQENSATKVVTWWKEPKTVVDLQYAIKHWSQQFFKFGGRLRREKAFRQLNFEPKTPERFVKTREWIKKQTEGQQDQPEPKQSQPIQHNRQIEDGFEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WC77</accession>
    <name>A0A8S1WC77_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tubulin/FtsZ GTPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0870233</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000087">
        <property type="protein sequence ID" value="CAD8186751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WC77"/>
    <dbReference type="OrthoDB" id="301794at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06059">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000044">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0493">Microtubule</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="5">
        <location>
            <begin position="34"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8186751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="425" mass="49686" checksum="A9309043301723EB" modified="2022-10-12" version="1">MSSAFHIHIGGAGVMIGDMLWKLYEKEHNETTQKNYIYQQIDDHHHPLALFADLDDRMIHEVQRNKQVQFKKNSFLYGQEDASNVYARGTYTLGREIVDKGLDFIRQQVEMMDRLDQFVITTSISGGTGSGFSELLLSRLNCDYGDKVKKNGFIIFPSSEMSNNILGVYNAMFSIQMTREYFQSITMFDNQSMYNVIDHQLDLDFVDYSHINNLVAQIISQYTGLRRFNSSDNSKFFSNMCPYPQMHYFIPSFGKMTLINDYNRKELDQSQFIKYLTKKELKLYQCPQNPRHLSTTLLFRQKEVNHFYGKFDLTLQNLDHYFNQSPRIFQCNSSNYQIIPEFAEMKKTGTFFSNDASIVSRFKLLGAQFDKVYAKRAFVWCYVTEGLEESEFSQGRESLANLENQYYEIKGVNDSPNAFYSNNEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WDL3</accession>
    <name>A0A8S1WDL3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0820087</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000082">
        <property type="protein sequence ID" value="CAD8183996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WDL3"/>
    <dbReference type="OrthoDB" id="290033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8183996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="385" mass="45270" checksum="79FC65B75CCECAEE" modified="2022-10-12" version="1">MFIRNEMKSALQSGDSFLLSNKSVSQEKMSQEQINQQSPKKRSLLKQKHNLIPLTDRLEIRKCYYNEQQNEEQKSQEHRLHNSLQKNKINLLPKLEGLESDKSLYQTVIEIFPADHPIWNDLVQKSQTIEEVIESNPAFFLGLMTLYVQQSKLQTLKNKFQQKAIQSQYQQDYQNVNLGNQDVLLDQSKAHDQMWGKNLKQLYNGSVYNSQYLPFKLEKKRKSPKKDFNPNLSQSSYLSTYRSQFQNWKPQYPGKMTRETSSVEPSNLPFISESSYAREFKNIKTERSPLQKIAKLGPFAENSEQLVKESTSSYYQQQQFNRIPSKLMRPITPKLFNASFEGQYTSEFNKSYHINSDQESSFANRFYESNSVKKIFDRKFDSKIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WH88</accession>
    <name>A0A8S1WH88_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0890117</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000089">
        <property type="protein sequence ID" value="CAD8187519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WH88"/>
    <dbReference type="OrthoDB" id="19692at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23795">
        <property type="entry name" value="UBCc_UBE2G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000051">
        <property type="entry name" value="ubiquitin-conjugating enzyme E2 R2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="3">
        <location>
            <begin position="17"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="1">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8187519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="178" mass="20291" checksum="95BB14C64287A38A" modified="2022-10-12" version="1">MLQSQQSHTAPIQSASYTVTLLKKQLKQLEESKTIEGVSVGLEGNSLYSWNISFAGPSDTLYEGGYFQALMKFPEDYPNSPPTFRFQTEMWHPNIYTDGRVCISILHAQDEFNDQEPPETRWRPILTPEDVLISIVSILSEPNINSPANVDAGIQFRDRPDEYKKKVRKLIDKALENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WJH2</accession>
    <name>A0A8S1WJH2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0940114</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WJH2"/>
    <dbReference type="OrthoDB" id="303267at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034044">
        <property type="term" value="C:exomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015374">
        <property type="entry name" value="ChAPs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31975">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31975:SF1">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8189613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="911" mass="107366" checksum="0196AD2A7D88F202" modified="2022-10-12" version="1">MLNLSIKNIFEFLDKKIDGVKENLLKNIDQLQGLGIPDICIITKQQPLNLIKSSFETLTSFHFVQGLCPQSQADVFAYLLTILQNQEKAKQTLFSKSQSITKVKYVSMQLTYLSYDSFSKTILVCEIQLEGEKKQNIFGVQENNKFILPTKQHWKGAYISNVLRAIDEDLKLASVGRFFNNINLKNNIKLGQMIHSLLELITINTQFFQDFDKIISKKCLCDFDDILYYICWPLGILVTYLVKSNQLLILINELENIQNNVAFYLIKSIIFYKMKQYQKSLSCLVKITSNYSQLNLVKYLFGKLLIKQGKFQQAFLLLQEILIKCSENQMIWITLASIFRKQNNYQVCLSVLNKAAGLKVMKQRKQTWIETYLQNYRVLTQDYSITNLDDLDKIYTLINPIEVDQIESFTQLSNYPILEKLLMRLRVQDRKELKQWHIQASKFVNNQAYKIGQYVYDFISQNELLQNKNKLSEFQKEIIKLSIQVGHKQLQKYLQYYFYYPTNCSSSQYDNNNIDNAFFEIRLKNKSVRRNQNDFVKRQNNNKSVSLNELDSDEEDQPEFLNYNLNKSFDFSESVSKTNLQKLNQKEILQNYKISHKIYPKLAKNNYQTIQENTFESVESQRDYVCKLKGIRNSLMQNNNIMQNFKEKKFNKAQKVQIYGLIHHLSNIITQQIQTEIGDSMKRKKDNLNPLLKEFIDYISQTQNILDELFYKNNQIINLNQNSQHKSIYNLDKQTSKQKLTQIFKSSLKESLKDKNYQQKQIDLRDFYTIYGFQFNEQNEQETYLQFTKQESIFLYNCSKIANKLKQQNLSQKLLQKLSDRIISIQVSYSLLDTYKDDHSQLIQIIQKIILDFLECGISKIVSIPIWVEIHLIRLIKLKGCNYVLGLLCTLEDIDTFQLLKKIVLQTENLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WPF5</accession>
    <name>A0A8S1WPF5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0970027</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000097">
        <property type="protein sequence ID" value="CAD8190610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WPF5"/>
    <dbReference type="OrthoDB" id="285097at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="270"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8190610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="318" mass="36296" checksum="F87A48BE6E7007AA" modified="2022-10-12" version="1">MNQEVDLYMITIFHIDNKLKTLAPIFYSIIFVIYLGNNIIFGELLLGKAEPFQYVTITLCCSIVINYCLIKLCSQQQVYVSQEIHLIGLGFAVLVLSALLSYKYNVMPFYSIILIKGWIAQAINETHLLGEMYQKKELICFIGISVGAILCTFEWTFQFLGILFGWIGAASMVIIGHNLKKVKKVRTHTITQYVMIYTITILGLYYPIHKFGTFNVELVTYSIINGVLFPLCCTLFLHGFQIGYPSMNLAIFMLINMSGAALQIGHQFDMLSLLGIFICLISVSYLLLNHKSYIEVAGFEIEAEENPYYELNQYHQRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WQ49</accession>
    <name>A0A8S1WQ49_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0960048</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000096">
        <property type="protein sequence ID" value="CAD8190299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WQ49"/>
    <dbReference type="OrthoDB" id="303271at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8190299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="292" mass="34531" checksum="F39AC5825E95B2E6" modified="2022-10-12" version="1">MQQYHRTFKSKASADRNIIVRSTLKLPQISIGMMNIPTRSSEHIRSSRLHTKTKTFIKPKERPQFKQFPSEKTMLTLGSLNSPRSEDEGDSTHKSQFSFRRTVIDTLSLRQSRRTSVEKVEKERYVEDKRIEIDKSSDKQALEEEVKDPTLEEHEIRPKKESQSIRPKNESVSSQFTQLTDEIHSVWDNMDLYLRGVQFDDFDHQNQLQSFLQLYKMRSGGFPGAKENLQNEEVIRRAVEYAKGNRQVFKKYFPTKQVVFENEEDYTQNYDVNADANFRKFHIYLKNYNQQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WTR5</accession>
    <name>A0A8S1WTR5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipase/carboxylesterase/thioesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1030159</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000103">
        <property type="protein sequence ID" value="CAD8193178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WTR5"/>
    <dbReference type="OrthoDB" id="2418081at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000644">
        <property type="entry name" value="Acyl-protein thioesterase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050565">
        <property type="entry name" value="LYPA1-2/EST-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003140">
        <property type="entry name" value="PLipase/COase/thioEstase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655:SF17">
        <property type="entry name" value="LYSOPHOSPHOLIPASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655">
        <property type="entry name" value="LYSOPHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02230">
        <property type="entry name" value="Abhydrolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phospholipase/carboxylesterase/thioesterase" evidence="2">
        <location>
            <begin position="23"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8193178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="246" mass="28005" checksum="938851569232204D" modified="2022-10-12" version="1">MPFNFHKLKQFFMFAKPQNLNDGSLVLNPKAAHKYSLIWMHGLGDTAFGFLDIFEQMPIVKAETKVYLLQAPQRPVTINMGMKCTSWFDIKVLKTDCNIDLFANNFKSTVSMEEIEDSKKMITSYIDQEAKLVSSKNVFIGGFSQGCCMALETGLSYPQQLGGIVGLSGYLFPTTQINDTQKETPLMLVHGEQDQMIPCELSKKSYQRLDNTKRQNFGHHIILKMGHEVPMPTIKLMINFFQNTQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WW03</accession>
    <name>A0A8S1WW03_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">STOP protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1010089</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM154 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000101">
        <property type="protein sequence ID" value="CAD8192319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WW03"/>
    <dbReference type="OrthoDB" id="365640at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033336">
        <property type="entry name" value="SAXO1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31516">
        <property type="entry name" value="STABILIZER OF AXONEMAL MICROTUBULES 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31516:SF17">
        <property type="entry name" value="STABILIZER OF AXONEMAL MICROTUBULES 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8192319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="280" mass="32055" checksum="84ED2338E5B92810" modified="2022-10-12" version="1">MSEQDYQANSRYSTLQSNNYQSQLLTTHFGKLLDKNEAFFNHLKGDCICGRCICGQCKCPKMLLSNDFKKGQESLYQKDFTTHGKQKQIPLIDHNVTYKSQLPMDINSIQRSTYQAHPDAKPAESLKPEQKVFLIPFSSSSAYRLDYTGGGMSSLKINPPHHPTVVDIPMTTHSTYQDNFYKKPIPREEFLTTANKSSFKSPISPELPFIHQSTNSAFYKPYQTGKLNRIDPEQKVKMIPAFEGQFNSTTQKDFDDKNPKKCPSRVFLNSLYKQKLQQIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WY60</accession>
    <name>A0A8S1WY60_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0970126</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000097">
        <property type="protein sequence ID" value="CAD8190806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WY60"/>
    <dbReference type="OrthoDB" id="674948at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14811">
        <property type="entry name" value="bZIP_u2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47416:SF8">
        <property type="entry name" value="BASIC-LEUCINE ZIPPER TRANSCRIPTION FACTOR E-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47416">
        <property type="entry name" value="BASIC-LEUCINE ZIPPER TRANSCRIPTION FACTOR F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="9">
        <location>
            <begin position="134"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="117"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="146"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="129"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8190806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="322" mass="37934" checksum="BFBB21DCA61650AB" modified="2022-10-12" version="1">MNHSFQIVPSDNFLNILNQNNISVVDIDDEYRVEQDWMFENENNYIEEQHEPVQPILYERSKALVGNQLLQKLKKTKEEDLLKQNKEVFQVNQSKSLYLNKIQQLIDNPAVVSKPKQTRQKVIVESDSSDGKGRLAKNRESARNSRKRKKIYVELLENKVKELTEQIKQLESNLQQYKIKNLQLESFREDYHKQLSQLNGLQSIQILQEQYGATSQQRWSVCTELINLLIESTIPIEIKKLMESAKNGTDMFIKPLLNHNSCLQYREYFQQGIIDLEIATKNFGLSYDKIREQVFYLERLKLEVIQTIGANQFIEYLNQQLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WZH7</accession>
    <name>A0A8S1WZH7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1050119</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000105">
        <property type="protein sequence ID" value="CAD8193940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WZH7"/>
    <dbReference type="OrthoDB" id="299469at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047126">
        <property type="entry name" value="RNF141-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109">
        <property type="entry name" value="RING FINGER PROTEIN 141-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="231"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="283"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8193940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="332" mass="39439" checksum="B2EEA38F03D5D087" modified="2022-10-12" version="1">MDSPVTIFSFIGSSATGFALYQLIPLLYQLKKKYIEFQQINEFTPTSLMNYFNQTQQTTVEAFIAGELISDSPIISKVPLILSEKKIYYIYENGIKKLIKTLQHGASQLSVADYYNKIEIWKSSTLNYQYSMSHIWDRFLPKQLSIFQRLANFILKTTNLIKTRKLFFKGFHTGLLEREFGIIAKEFYVIYGEIILDKKLNKMFLQNPKYILKSKKQLLQLIQQQIKFKRITIAILVTIFTIWTYIFGCSIKNLIFKFLKDREKKRLDKLRGQKYLEINNYECQICYERPRNIIIKPCKHLTICHECLQRFKQSKCPICKQQIEDYIEIFFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X361</accession>
    <name>A0A8S1X361_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1080079</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000108">
        <property type="protein sequence ID" value="CAD8195009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X361"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8195009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="139" mass="16751" checksum="2195014C3929E60C" modified="2022-10-12" version="1">MHQKLYLNKQDLSPIKIKNAIFLNKMQQVLNFDSSNKMSYRIQIKLQFIERKLQQKQYVKQLNISYYFIWHIGFMIAESPDLLSYLLAQDMETVTLLYFIKKEEHSNRFSCLKYNLISPSKKLIKIFHESYCICQNNLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X3A0</accession>
    <name>A0A8S1X3A0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein-tyrosine-phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1130021</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000113">
        <property type="protein sequence ID" value="CAD8196543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X3A0"/>
    <dbReference type="OrthoDB" id="10252009at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45948:SF2">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45948">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE DDB_G0269404-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="7">
        <location>
            <begin position="61"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8196543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="15940" checksum="3EA695DD04E84A37" modified="2022-10-12" version="1">MSEIVPHVYLSSIVYAKDLYWLKKKKISNILIIGQLQKYFPLKFQYKCILIEDKPENDISQYFEECIQYIDQVIAQNKNILVHCYGGQSRSVSIIIAYIMRKLSIDSEKALNFVKERHPRAEPNYGFLNQLKTFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X4D0</accession>
    <name>A0A8S1X4D0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1130174</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000113">
        <property type="protein sequence ID" value="CAD8196843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X4D0"/>
    <dbReference type="OrthoDB" id="6612291at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF2">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="322"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="348"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="383"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="408"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="11"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8196843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="452" mass="50771" checksum="782CDDF89A2BDAE7" modified="2022-10-12" version="1">MYKSVTLLKHLVANASLGFVYFGYSVGYLNPALQTVDIVFGITENVDYFNGLLSAIMTIGAAIGVLIMPNMLQYFSRRQSLIILDFFGIFGYGIQLVGDWNFLVIGRFVAGVVLGLNSALDPVYVNEFSPPELQGALGSIMTMSGSFGLFLSFTIGLWYKQSPTIDDQYWRVGFSFPLLVCAYRTYNLISNFKLDSPNYYISVNDETNAIESIKMIYQEQYIQDVLKQTKQNQKQNTNQNESYKDLFYGRLFRNLVRISLLACIGQMLGINAVIFYSTQIFNEITNNDVQKSNILNLFIPLLGILGAFLMGFILNRMTRKNFFLLGAVACVLVELPLGYMTSLQEQPAILIVVFILLFILLFSTTIGPAFWVVMPEMVSPKGVCLATSVNWLGTAIVGQFFPILVSSFSISVCFYIFSFAGILGIIYIQYDFKTDPKNVDTQQNTELVILNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XBD6</accession>
    <name>A0A8S1XBD6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nbr1 FW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1190059</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000119">
        <property type="protein sequence ID" value="CAD8198556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XBD6"/>
    <dbReference type="OrthoDB" id="299024at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032350">
        <property type="entry name" value="Nbr1_FW"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16158">
        <property type="entry name" value="N_BRCA1_IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nbr1 FW" evidence="1">
        <location>
            <begin position="96"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8198556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="248" mass="29343" checksum="F54E214161A37B5D" modified="2022-10-12" version="1">MINKIIMSFKYYEAFVSSKNKSIPCCNSLNCNLCLGKYYIQVQNEDINLMDILIQDKLQSRMKEIQTKLKINTIDQFLDYHQRKYNYQLIYLFENHTKGKPEQQININLKYKNNGTIDWSNETYFKCIHPEEFKGIISQVTPLQSGAEETTEISFKIPLIKQGEYLTKWRLCCKRNQEEIQFGPTIEIPCTIEESIETFNYLVLQQLKNMEQSVIESLDFSKAPKILEDNKDQKLDINYLFLLLLKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XBT1</accession>
    <name>A0A8S1XBT1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1190036</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000119">
        <property type="protein sequence ID" value="CAD8198510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XBT1"/>
    <dbReference type="OrthoDB" id="314008at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8198510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="62" mass="7729" checksum="DB055FB17AC9FFE1" modified="2022-10-12" version="1">MDQIAAERIQEFEQKSGMKISEFKTENPIKEEIRPEYIERQKRIYRQKMEETRKQLQQQQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XJ04</accession>
    <name>A0A8S1XJ04_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1260025</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000126">
        <property type="protein sequence ID" value="CAD8200857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XJ04"/>
    <dbReference type="OrthoDB" id="312663at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="2">
        <location>
            <begin position="451"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="667"/>
            <end position="694"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8200857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="911" mass="109980" checksum="74FFF4F053F9147E" modified="2022-10-12" version="1">MIGLQEQREILQQQNNPEQKEQQQFDNIEQATISRIFKELEEIRQFQKKRALEIKKNKDLCLFYFNLTEDQNCQIELKEYIKPKIIDQWQDGFIGEVKDTQIIVYFQNLMQNNDKQSEGLNYYELSKIKLFMKSFLQQNKFKKLNFIVQFNNEENQEKLNQKIKHRIFQLFEGFFEVQEEDQDIFIFCLLNNKRNNYGHQNECQENNSYTKEGYIYSLNEKEKPSKPQPINSSQITIEYFNQISENKINVIQMDWYFKQIGFSCIQYIQSKVNIVFYEYSQSLYILRKQIKNLLEVQINLNDEANLNEYIREYLNNLKQLIFKIKKYINAEKENLNFQQLCHNLKYIISYYLKKLVENLNKIKDIPNISWKIEKYNCLNFNVDFTNNDMNLLDYFKEYYTFDYIKEIFKLLEKSLVFWLHHYESFIPQRLSYISNFIENQNGQNNQNSLNVVVLGITRVGKSTLLNIIHDPNNMKLIQTSSGRYCFDVINNDKLFEISHENNSKTSSINEFQINIGKSDLKFIDTPGFIDTIEERRLINQIKMFQRIIELDKKKTDLQSTIKHTNSFFDEQLNIKQLKQILIPVFIKVNEAIMGEIKNKWESSTMQNCNSEQEKEFLRVIKNKIDNNQYITLFKAEKYFISSLEQLKIDKQAIIDKIMKEKHNEESQKEIQDKYQKIELQIKDLEVNKEKIEYQIINSDINKIRNEIFTQCQVLFDQQKKQKVDFNFKLKLTPSMENSLYIVLKHLSQINNYVDELISNAIINDILFKQRPLEKIDDLLKYVQVQLNRFDQQEINSEKLWLELSKYLDLPKDQEFLYEQLSQIQKLEQLNKFGQKFQIELQQFVELKQKIQMLQEIVKDTIEIDIKNDIKKNMSFFRWSKEPTQQELEFEIRKQDHKIQELIRNKYLDKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XK47</accession>
    <name>A0A8S1XK47_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1270001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000127">
        <property type="protein sequence ID" value="CAD8201214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XK47"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8201214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="51" mass="6113" checksum="2683F4CA1BC5711C" modified="2022-10-12" version="1">MKTLQLKIEQFNLIFSRNEAIIQKEIEQLKLDIEKETIIQGSRLLEKITQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XMX8</accession>
    <name>A0A8S1XMX8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1300038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000130">
        <property type="protein sequence ID" value="CAD8202094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XMX8"/>
    <dbReference type="OrthoDB" id="306159at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="168"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8202094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="196" mass="22612" checksum="5F0090644A7B7754" modified="2022-10-12" version="1">MIIPLQMDQSLVENSKRKPFSPLEINPKQYSMSSKLNLLQLDIITPIQIKSNHSNSNIQFGLQKLLKTSPYAQLIKQKSQQQISSIDTGSQNVYNGTTQHSSQTNVKVDTKFMMTNNQNNIKRRIHNSMKIFAPKKSLEYSHQMNSTYKDKQTIKALQDLLIRSTLTLQMYKEQIIKSEQENIKLKEQLSGLQVQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XS38</accession>
    <name>A0A8S1XS38_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1340024</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000134">
        <property type="protein sequence ID" value="CAD8203488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XS38"/>
    <dbReference type="OrthoDB" id="298049at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8203488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="803" mass="96054" checksum="249B59056A0F4890" modified="2022-10-12" version="1">MKGFEIELDLDAEHIMWMYHLKFFIDLKRKYETFVKFRGNKAFLEGGDQLRVQCAVEDALFKAKSEFIYDFLMQSNNPEENYGPYLELLSNQKYEQIKKEYYGLFIFTKRQFMQIKQDMIDKFGYLRNWSRQYPQNYVIIFLPSSKQSQLIQRIQDLVRHINNNIDQGITPQSSKYYSYRNRDLAQRNVYKKIYQIKMRDLQAIQIFLIFDIRRMEEYFKVSFTLKDYHDYVILVIQGSSDIGCQEAMSTLENHLKHKILFQIQKEQDNLFKGTEWKIVKIEEIELIANRLDQCFETEILKRMTNNFQKNDFIFTDIKQWNYFRQKQMNEESNQISRLTNSYYQSEVDRQIDSELIQQDISGIGQLEQENQKEIFQYKNNQDGDSDIIIQPINQQINKQEIIQQIDNQSSEEEQSDMKQLKIIKDHKVETLQDKIIIEFFNYKEKSKLYLFLNNFDEGQFQSDFDAYCSNNYIFPYVIQPDYSQQNQLKFIVEIFTNDDKNNLDQIIDFLDHYMNCQIMLGIKNIQQLQQVDQEELEELCDDYNCNLKIYNCDLSAKQVGWQEIDAQPQKNQNGKDTVFIFSNVMHCFIEQLKDKVNSLNQFSKKSIITLLFQSQFEFAYKLGEIKKLDKFGQFHLDDCYWVKITAKNPKEIINNIKENDSKIFKTILGKQPLCQNEAIVLGKADENREFYDVFIPELYCKYYEKLRSDYLEHQYLEEEWKQVKIEPNEKSFFIDVTDLEIPPTHLEPIIEVIAKFERKGKVIESITNLQKGVYLIGKTNNVNSDTFELTMNNHVISSIINIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XSY1</accession>
    <name>A0A8S1XSY1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">OTU domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1360075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000136">
        <property type="protein sequence ID" value="CAD8204175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XSY1"/>
    <dbReference type="OrthoDB" id="415023at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22771">
        <property type="entry name" value="OTU_plant_OTU7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050704">
        <property type="entry name" value="Peptidase_C85-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419">
        <property type="entry name" value="OTU DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419:SF7">
        <property type="entry name" value="OTU DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OTU" evidence="1">
        <location>
            <begin position="17"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8204175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="191" mass="22610" checksum="93278EA504E42E96" modified="2022-10-12" version="1">MKKYFNQFNIRLQQLGLMLRDVEADGNCLFRAIADQLTGNEENYNKYRSMAIRSLQKNRKFFSDFLPEDFTFNEYTKRMSEDGIWGGHLELQALSNSLQIDIVVHTLENYYIIKHIPTKTCWSSFKEKIHLYFNKKQVPIQKQIHIAFHSGKKIINHYQSVRLIDDNTNKPAKQPQIYCELSEQSTTDESY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XT35</accession>
    <name>A0A8S1XT35_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1360100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000136">
        <property type="protein sequence ID" value="CAD8204225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XT35"/>
    <dbReference type="OrthoDB" id="312007at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035801162" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8204225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="467" mass="53654" checksum="56CDE7193916656A" modified="2022-10-12" version="1">MKEIIVFIQFIVIAISQSQSNDQIDCNKYLSKFGTKESPLAFSGDAIYFQEFNEVQINVKYTDILFDDPLYFGLVQEDGKQPETTCLKLKVLSFTDTHHQSSKQLSDLKITTSNNTINKWRQYQFKIPASELQSNIIDNNIIYNGFYSFIMETKNQEAIKHEFNFDFTLTVNLPSQIQIDTSFKFQLKDQLTNINPPQSKLLWCSNITCQSVLNESPFIRLNDTFVIKQVIIDSGITNQYLNNTQVWYIGYGLNKKATPIQIINTTPGQVILELKAEIVWQKISINVTSSLSGSPSINNVENIIETSFKPLDSSPEYPYQNDSVIRWCSDISCQTFLDEIPNLHLNDQFIIQQAVINKDYDDFFISDTEVWLIGTGLNKKAQPIRVINNVQKQVIIELKAEVIWTNLTIKVTSYIKDSSQQISGEINQIQCIKPEGQETCGTYECEECYNYQFTAFLNMILLFLLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XTL8</accession>
    <name>A0A8S1XTL8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1350120</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000135">
        <property type="protein sequence ID" value="CAD8203962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XTL8"/>
    <dbReference type="OrthoDB" id="307192at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="36"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="80"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="178"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="289"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="36"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="52"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8203962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="368" mass="44623" checksum="E7000D8BC9EC576F" modified="2022-10-12" version="1">MQYTAPIERRLVFQPSQQIMPLMHRKENIFSPLRISPSTQQHFSPSQHQFSKGNEYNQQKRQNTNDPEKEKEIKILIQHNQKMKDQINQQAIDYENLKHSYDKMYQDQLRLINELEQISKVSEQQLKEIKIKQEVIKDLQHQLESQCHSQNKNQDETQLLRQQLQFKDQEIFKFKAILEEKNLENQEIRNTLNTINQKVQFMTQQKDQQYQIIDKFNKELQDEITHKTQQIHATSQSLSQSQQQVTQLKFEVDSLKVKLQSQQQLMPLQQLQQRYLMLCMENDRLHSIIQKEYELKKQMEQQQIKYEQETEQLKNELKQISEEFSRLQQGLVYEQKNMYLIENLKNQLNYYIDENARLNVIIRQQSRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XUU4</accession>
    <name>A0A8S1XUU4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PLD phosphodiesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1400086</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000140">
        <property type="protein sequence ID" value="CAD8205306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XUU4"/>
    <dbReference type="OrthoDB" id="14911at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030572">
        <property type="term" value="F:phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21248">
        <property type="entry name" value="CARDIOLIPIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21248:SF22">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="1">
        <location>
            <begin position="125"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8205306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="422" mass="50965" checksum="1109DA48AFF11F55" modified="2022-10-12" version="1">MINQEFYDNALKTLQQLSPEIQEYQFPHLRLFTDGDEFYQKLWQMIDESKTYCWILTYAMDKSLAANITLYKLINAQQRGVNVVLFVDDLQGHYNNKLTKLFQSYGGVFLNLNPLSAFKIYGQEFFRRHHEKMAVADHKAIFGTSNIESHYGGVKWGKQTFHDINTYTEGRHLSYNLQKHFMYLASLYKIQLNPHKPTPNEDLLYTFNAFPPYYEFFKTHPPFNRQIQTQIIESIKNAKDNIKIVQAYYYSVRIFEKYLREAMDRGVKVEIITADKRDQPVYRYAFNRILFQGVIHRNLKVYEFPEQLLHMKGYVFDNNQLFYGSFNNDRWSWKLNHEFNILTEDENEIKMFMDIYNETKLRTRQIDYKKTGTPLRWIKMKFWEWFIYQSEILMSKNQGYHPNKYKYNYLLNTWDDGIGNYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y0J7</accession>
    <name>A0A8S1Y0J7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1470124</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000147">
        <property type="protein sequence ID" value="CAD8207511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y0J7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8207511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="182" mass="21792" checksum="A687805D3823795D" modified="2022-10-12" version="1">MEEIQENHDKTNTNIQLEQLIFQQKREKLKFLNKISQLEKQQRMLNSQVEQYRRETDDQKMKIKQFTNLIVKQELVQDKMNIDTSLERQLIIVKNNKSQVFIKCSIDRNEAKQILQQLGAKNKNESLDEKFVKENRDFGYQDSKQQFPSLIEEQNKLVVVQVHRSKLKKMTSQQVEQLLNKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y3B6</accession>
    <name>A0A8S1Y3B6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1480110</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000148">
        <property type="protein sequence ID" value="CAD8207767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y3B6"/>
    <dbReference type="OrthoDB" id="293474at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001088">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="32"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="326"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="378"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="383"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8207767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="495" mass="56972" checksum="38D547492EBB2346" modified="2022-10-12" version="1">MNNQECCYLAQEINQGWNKCYDAINLMINDQYLIKKKISQGSFGVVYVGQDVQTNQYVAIKIEKNDSGSSLEKEAKILQHLNGTLSIPRLYYFGEHKKQMLMVISFLGKDLTYYIRQYKKFSLKCVLLIAEQMLSILENIHLKSVLHRDIKPENILTGRDNDSNIYLVDFGISKFYRDKKGRHINFCENKPFLGTSRYASLGAHKGQELSRRDDLESLGYVLIYLLKGSLPWQVLKLQDAQKIKAVGDMKNTISLHELCSGLPNEFERMIDYARKLDFKATPDYKFLKQMFQHLAHQQNIQDQLRYDWDYTPSSLQVPSSEIAVLKRSRRTTDQEQQDLQQNRKKQASSSTLNGLDGLSGHSFLKTPEQQCRKISLTPAKQKSNSSFNDSFSSCNQSGNQSVIGMPSHQNNLSQLSFFEDHNLLSLALESMSKVTKEQLNQEEHEENNGELSPSPLEVKYLKLKKQSIHVHFKRTLLQIESMNILPPQSSFNQCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y7B1</accession>
    <name>A0A8S1Y7B1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1530112</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000153">
        <property type="protein sequence ID" value="CAD8209193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y7B1"/>
    <dbReference type="OrthoDB" id="297368at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="491"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8209193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="651" mass="78431" checksum="4DC4CF343B789693" modified="2022-10-12" version="1">MIYKPDLLQQQKQIDRTIKYVIYEGINYQVYENKSGHLIKQYKFENIYDLNELIKTLELEQYLSKQTDILYAPQYYHIEENYTIYIQYQLFYNLLRQVIQQNQSETQKQKEDQELYNDLIDDNEKIYEVNPLDYANELQVKTQNLNVTQNLINTIIKLYELELNGFMIKNIKPSNIIYNNENLQFMDNFLQSIKFEVSLEEYLILDFIKQQYQSNNQSLKNLGRCYMQLYLLIEIEDQQETEYLELINKYYGSKIYNLLRLMIQSDYRYFTSIMQSQEFLDLITPLNILDNKQLFIQFFEKKQQKKEEIWERIKRNFEIDQQQQIWQKYQKSNLQFMIKEKEIAKYRVEYKVSHIHTHKLEFDITDMVLINQQINQNYERAILLTTIQGKIIVHSNTTILNIFQCSLKSLRSAIKFKLEKNIYVAIAGDDKIVYVYNQNEFQNSNPKLFFQIANDTTKQIYSLLSYDKFIIFGDEVGSIYIYKNWTFSTKLNAHSKRCNKLYYSNNQLLSVSHDQTIKIWDSFNFLIKHIITGHTSNILNLFIFDNYIYSFSEDKMIRFIQFNPTTQKYDFVYQTKFDSAIQSCIQYNEQIMIFGNKAGQLLIYNIKDRIILHTHKIHLNSILAIYWISHSQGQFITIGQDKIFRINAIQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8N3</accession>
    <name>A0A8S1Y8N3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1550070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000155">
        <property type="protein sequence ID" value="CAD8209608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y8N3"/>
    <dbReference type="OrthoDB" id="290784at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8209608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="184" mass="21635" checksum="EC8EAB2DEC5AD480" modified="2022-10-12" version="1">MTQSKQLTLEKMKLTHVQAEQQFKFMEIEYSSFNEQNKQEFAQKFRQNKMLYTECQKKLRDLQSDIDKQPQSHFNTVVKKTQPIQISGLNSDLHSQVNQLNHLDSRLLEAEDTQISILDNLRSQKEKLLKAIEDTKEIQSGVKMTQRHATMIKNRESYNKGILMTLVCILTIGNILIIYYKFVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YA35</accession>
    <name>A0A8S1YA35_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Autophagy-related protein 27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPENT_87.1.T1500100</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ATG27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000150">
        <property type="protein sequence ID" value="CAD8208354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YA35"/>
    <dbReference type="OrthoDB" id="10252009at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018939">
        <property type="entry name" value="Autophagy-rel_prot_27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044865">
        <property type="entry name" value="MRH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15071:SF13">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15071">
        <property type="entry name" value="MANNOSE-6-PHOSPHATE RECEPTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09451">
        <property type="entry name" value="ATG27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51914">
        <property type="entry name" value="MRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0072">Autophagy</keyword>
    <keyword evidence="14" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="16">
        <location>
            <begin position="16"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8208354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="266" mass="29776" checksum="075E1683138989AE" modified="2022-10-12" version="1">MFSLLFLITTSYATDKNCLWHDAYGYTYDISKLHNINNYQVPDTDTSMGMFNMVYEFNFCTGAVRCQGRDVAAFEALEVMGKVTENCDVLGLKETQEFGYVNPIYPELGISITYKQGDMCFDVKDDAGLQKILADNEQMQMSPRSVTFEITCGKDEPNFRVIDSSKCNIRLGIQHSAACRSGSSPSGSSQTLLLYIILIAGLYFGGGIAYNKKQYQLSGVDALPHSHFWRDFPHLVKDGINFTIIKLFGIIKRFRGLSNEGGYAVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YBG9</accession>
    <name>A0A8S1YBG9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1580029</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000158">
        <property type="protein sequence ID" value="CAD8210147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34491">
        <property type="entry name" value="A-TYPE INCLUSION PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34491:SF129">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_47, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="9"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="50"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="92"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="163"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="387"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="806"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1631"/>
            <end position="1658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="163"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="175"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8210147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="3074" mass="362376" checksum="61158C25358A5C66" modified="2022-10-12" version="1">MDFLKDNKKFEAMVQDVFHTIDENDSGYIEFSELKRTILEMYKQANVKQINDEQLQDMINTFDKDDNQILGQEELADLLRQILEKSVQIQKEEEQKNKKKADKNEPTTFRSKKIITKDREQQKQEIQQLKQELNSLKKQDKKSQQLQNQKLVNYYVNSTARDEEVREQQEKFDASSKSLSIQSPHSSKSSINLNPLVFNQYLRQGFIKNADDMIKAQESAKNNHLIGLAVKRSSAGEWQGIIDYFNEQTQKEFDRTPSSQQFHIISKKLTKNVKFIMIHIQNLKYTNFRDLRINSKSELSSVDKPALFFWELNYNGQTYFKNSAKEFPNQLNECLDTIIIYNYTNPIQVIFWKKQGKDSIYVGQFDITNQLFQLLQKVTDQNELGESTKYNLNLNKINKQLRKLQSQEIKLYRKKRVNYSNNSLEDPPLLGDIIVNFQMFEICEFDAPINQFSYTKGSPRSLEERERRMLVEQSKYGPYKWPDDQHNKALVVSINEASIELAIVNEEDIQSYLDGQLGALIKETLYLDIDQVGSRRTSRIKRPQLSENTIALLKSLDENPYVVQVNALRQTIRQGRLPKKVLEEALQQFGCTGSQLLIDHVDDMGMCEYDYCMDDGSYGVIIDTIQDMEFSNPENIQQADIFAEKLIQNIKSVLIEGIRIEPLFEFLKVAQKIPDFGITINLISEQFKNGLGSKIDQEGNVQIDAGSFISYFDINSRDFDKMENYEHIMVLGKLVKLAKKHLKPDDPFLNHIQSNYEEAKTKIYFTVPVLPKKKNLKDKAKFLIIQSNNVHPGLFSNMDINSKTPLSEIKQKFLQKKKEIQNEKENIDQEQEQDSAKLLEKPCLWNICHELVRRKRWNMFYEGFQAEPLFYFQQGGTGNTPLMDFFEKGPFNVINQMITQYHPNNPNEDNKDIPKLDPKKIFDMTTPQGKSFIHAIALNQDINPIGQKIALQSLLGLVEPEQQEQLFVTTMSPYDATPLVLYITKLSSVVPAFNTEEIKSIQEMLNIMKQPLKKDFNQYEFRAKCFFKIHNIQIEEDDLKDYYYNHIQLSKAMSLLQQNMFEFVSKEIDWIQWFSLNIENYDGVTKEMPFAIYNLARQGKIEFLESLLENFLKQQSTMVCAIKPDLNFIISNNQEDFISDENGQITIYDKDKNKTIKFSQDDMLKAMAQFYAKYPLEVFIGIQQILNETKFTTLKLNRLLKEITLQTIKIEPRILAKQFYMNNKSPFEELVKLGLQEELQKILTPEQIQAYYESKNNFAKNLLQEGLLGKLKNKEDPLNFGQFREREFLNYTSKQMTEKGVEDVLKFIDKLKQDNIDGRQRLIQQLLNQIASSLPTKFSKQQYPKIFKALKSNKQTQQSDDKSNFYLRNPKKLDKQSEVKNTNGYLMKQILNAALYSNNQQLINNCLQYIWNKSPDDLSLRNLSVLRPIYQKYGQFYRSLTQHPAVCLNQQDFDKYMNDTKDVATLEDYDIAAKALTKLGLPDRLPIFLKRLAANKPELVFNLLEDIALNILEKQLREVPVVPAKANQEQVQKVQPQFIIPTKRQIILSQYSLFDAGALGYLIQNAKKLLEKCANRPKNWNAYNHTLNKQAFQQYYFQKLRQNFYTQNPGTMYSNWNAPKSNIEKTVETILDALKDSLDNLLQKKPELKDKLEKISLLPIISNNIQILDKLNNKEQRIFTAIENIILQNLKQKASKLELIRIEKRIKMNLQFLEENFKPNLKTVIEQEKNRSFRIFRALDYTLKKEYQPLQLQFATDVILIIVKNFVANDFSGIFDKNVSYLYNALAVSLMRSKTTDPRALEFMGSQLNKYQFYDLVHSLELGEKEGNEASQGDVKGKKDKVDEIQRIKEALIFRNIETLPTKTEIEPAGRLVVPSQSNIPYFIKLLKAKVKPFFQTYNKEFNFVNGQPFEIDNLKTLNLFEYLIVKCHYTLISKHYINVFKPKNPLQQDQIIKFLCLASSCGSYTLFDLITDQILNLKFPQQYKDQQKFPQIQQYLNLPIFYQVLMYSSEQSSTKFFENIIVDGVDLNLFYNWMKFNIDEIENENISEYNCFVLIIIKKYGMLFNKIVQFLRKLLKNDKEYYQNLTEIMDNRNFKESNIINEDEENQESERLSLLEFSIYQGFPDFCMVFDKNIKLRRNLFIEKVFSILQKSMNQCSSELKQDDSVLINYCNKYEKDQGNYRVQHYKIYLILKKYFEGNLTIITSLQTSVIFFKIMLEYKPDLNLLLCVNQQISLTEDIENLQIFKLLFYEQFFYLHLCGFDKVMKYQLSISDYVRQFHSKQKLFWASQEVIDAFKTYFKFDYKQELKMMNQFEQSQLNDQLDQFTPEVMLYLYYNQPDLVERSTQYNEPLKSFSSYKQMLPIIRHQSTQEILQPIEQKKMKNHELQQIVEKQQEHFNKVVGQVIIEDVESNYQYAAILVIYISTCNDLINYIGESDVTNYKIVPILNQILERLSIKDLDCFIVQLIFSIRNNDLYNIVMEWSQQSKAAINKDIFKEKSFIQGLLNLLNVKRKSQNYIINYQQNDLLKLFQFEFAKTDKMCKDKIYRGFCVLHIVLNFISEIESFIQNVNPNDQITINIELMEQSKGKNIQLKVLSIQDIIIDKDLTGFNYQLEIPMIYQEKNNIINLFINQSVVKEFQELSKKQIFDNIRLKEFQEELQNQQKFINSFQVQFAIKPNQLQYDILQIINEQLSEKYPFEFGRQYGNIVHDITLDKDIFFEDENNNKIIQRKNLSIEDEVNVSAYFNLTTYQYEITLNVLILKKAQYFSKCNRENQLKQLIDDFARKQSQLHYIKRFLDVDGNNVREYQSMMELIKEQKIKKKQKSQLSDYELEELKILQEKYQKYQVPPYFVYELDWPIYIENKKIYADEYNFPQLLLKKLISIIDGVFFETVQSENDNKKSKVKTIFIKEQSFQINYISFIDTLVLQFAKILELQLKSRMMQLSLEDVAYQALEWPFLKYIINVIKSKIEKSSLLFEMKESAKPEISINLPGIDECLQLSKTTYFQYNGMFVVRLGVYFNKKLLKLEKPENQNLRYSQNFSLDFNLEPYINYVINEPQLKAVLKKENNNNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YD45</accession>
    <name>A0A8S1YD45_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1660027</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000166">
        <property type="protein sequence ID" value="CAD8211985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YD45"/>
    <dbReference type="OrthoDB" id="309438at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="279"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="356"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="279"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="304"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="363"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8211985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="457" mass="54522" checksum="18793A3922BF2F95" modified="2022-10-12" version="1">MNFLNKIFASSLNRGSFQIEDVSGSDEDQDEIPEHQEIPQFKKQSIQQLSSYNSRKKPQSKMGQADFLAKYDENFDDVFGDKPPPIDFPKGRKRRQEDDEEDENDNDNERKSQKKKTQSKERKQQSKEQKDQSKEQKDNKKITKEFRETIDYKEFNKEYKEPIESKIQKEPKIRLPKFPKQRKRIANNHLSTLRSNSDGRDSVSNKDRESIQLNNKYNPNDIALPQTAIPKSEKKQDKQKTMFFTFGMNDDDDSDSEKDIKNKINELFYDQKQKIDQNEQQSNNNNNNNNNNNNNNNNNNNNNHLKEKKEQEKKKQKNDEKKDRNEKKQRKEKKDNDKKTINHLDIFDEIKDIQKNFNSKQNSEQRETRKLNSEQREKKRDKKRESKQNRDKNEKQTSKSPSPQLKLIQYETQKKVKRSQKRVVQDLEMLNVDELSLNPNIVECKANLYLDYIPVYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YDB9</accession>
    <name>A0A8S1YDB9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1680065</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000168">
        <property type="protein sequence ID" value="CAD8212476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YDB9"/>
    <dbReference type="OrthoDB" id="10252184at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8212476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="199" mass="23644" checksum="BEE7A22520AC7196" modified="2022-10-12" version="1">MIRKEVRLLLLSAKNFIDQLPQQKQQRASLNLMKIVQENKPEDQIIKEIHYQINFLKLTNPNIPFKQITQEDEITPESIIQKLNEQQEQQQKKEDIDLKKGTYIYQNGNLVQGQAEKRKEVDYSNWYAANVDPDDLKKHKELLDRQHFSGPFWEGKPMPKSILDEDNPNTLMFSDERPEKELNPNTKQEKQGKFERVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YFU9</accession>
    <name>A0A8S1YFU9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1680044</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000168">
        <property type="protein sequence ID" value="CAD8212439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YFU9"/>
    <dbReference type="OrthoDB" id="271226at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001215">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001441">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18849:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 410-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18849">
        <property type="entry name" value="LEUCINE RICH REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="359"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="359"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8212439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="394" mass="44786" checksum="6F8E76A18295EBDB" modified="2022-10-12" version="1">MSQQDEGEEKQDVEQVEPVEPDEPDVPQEEYYAPESLQIQKLHNDIKEGLKRIGKTANLSGYTFVELICENKKIDKLFNIMQNYVNLRKISFANNQLNDVNSLQSIKYLTNLNLSFNQITNLDSFNVPNTFQFLDELNLDNNKITNFAQINLPRLKKLSLKNNLIKSAQGFNGHNTLEILELRNNKLESFEGFQNLLKLKQIWAAQNSIISIGHLNQLPELHTLHLRANKIIVLTDIPNLPKLHHLNLRANLIEKLDEFNNLKSLESLKSITMHENPIATEMGDGIRKEIIMILQQIERVNKEPVPPEEKADALAELKERIAAAIQKKKEEEEEAARLAEEARLAEEARLAEEAAKAAELAAEQAAAEQEIADKAAIEGKANQPKVEQEEEEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YH14</accession>
    <name>A0A8S1YH14_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1870003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000187">
        <property type="protein sequence ID" value="CAD8213806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YH14"/>
    <dbReference type="OrthoDB" id="6252103at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="300"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8213806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="518" mass="61074" checksum="721F0FDBB4625865" modified="2022-10-12" version="1">MNCTRHIQFPITSICIAPHSCQCSRKLCIDCQYEHGVDIRYTIPTHRFQDLVIKQLQDSRIDQNSEFTEQRKNILMILSQTENMLKNTWQHLQESIKQIYDTIEMQDKSYLKIVNKDFNPTDFSNTDLEKLVQIVQGKILNEWKAQKDFQLQQLLKVKDWLEKEIKTFNEKLMKDMDENLSFNFKVKQQQNEQKSQSQLNFKPFSYEIIQTNSIKQQESCNAVAINKDCSIVAAGCNKLIKIYDFKQGMLILNQVLNEHKNDVVILNFMKQSNQLISGDNGSILIWTHNNNNSWICSQTIKGHNYWIRCIILNHNEDLLISSSDDRTIKFWVKKNEWIYQQQIKDHSSCVNQLSLNEQQNQVISCGDDRLILVIEQSQPNNNWIVKQKIQVDCYGYRLCFTNDNLFTFQPKKGNMMYVYEMNSVSQKFIKSKDITINQGNEGCLLFPQQFNKQKQLLVNKHDKYVNLIRFTENSQLKVEQSIQFGTSSLVVQLSDDGEYLITWDQKSNEIQIRRFKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YH25</accession>
    <name>A0A8S1YH25_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1770023</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000177">
        <property type="protein sequence ID" value="CAD8213365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YH25"/>
    <dbReference type="OrthoDB" id="10479956at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="329" mass="40132" checksum="CA95BD246AA61843" modified="2022-10-12" version="1">MLLFFYHYKSKIIGYPSQITQFDDKNGVVSQIYNSDLIISNITRNTILLTKKYVESWIRIIFHVNQTFTILDEINKIYLKYQYRFNFNESQFFISSQDYAFIVQDKQTLYFYSYVKTELKAFKINFEKKINYLVQQGNQLFIYFQNCMIMIVEFQNFQINEVNHSHQVNCTIQLNDDSDKLNIKIQPSQIDILRKNTYTYSLRFHQIIRLQYFINYKKLIIFELNENLLQMKYYQEKNNYYQFLYLIPTYQYIIDQPLIYKTFVQMIAILAHNEQKDQYILIYDLHNKALNSMVSAIQVDEKCQIFNFHSTSQFFYNLIFRFHKNTIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YIX3</accession>
    <name>A0A8S1YIX3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1770021</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000177">
        <property type="protein sequence ID" value="CAD8213361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YIX3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="151" mass="18010" checksum="6708D7DE19C743E8" modified="2022-10-12" version="1">MLSFQTCKGIIKLFDFYSLGRTNNKNKSIITLQVLSYVSFQTFLGQTTHPYQFEDKNNIYAFSKTIDVIQSEEVVQTYFHNNLQNKNLYRQIRMENVLTYEQKYRVYLSYITNALSYELIKNQDSLKLQFKLILQINLIDFIKKILNVLFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YLG5</accession>
    <name>A0A8S1YLG5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T2090001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000209">
        <property type="protein sequence ID" value="CAD8214358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YLG5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8214358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="227" mass="27998" checksum="3BA91015667EC2F4" modified="2022-10-12" version="1">MDLLIQNQVLPQIFLYTLQKYHDILNYFFFLQLVDSNYFIRYQTSLIRLFYLYIIDKQKCSEILMTKNMYQVIKVIFLQIWKLIQDYIFIQEIHLHLQLISQRLQGKSHQTYHIHNVTQFYCYFILYSNYTNKIFQLLFQLHNIFHIQIRVMKYFSILDRIQIVCHKQMKNFHILKIFYYKSFSTQFDSNYLGIFVLFEDLFTNKHIQIKQNLLGNLLKFIGYQIID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RXZ9</accession>
    <name>A0A8S1RXZ9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0020146</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8132938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RXZ9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8132938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="88" mass="10355" checksum="BEF051384E85AD3A" modified="2022-10-12" version="1">MLRKISQQQQEQQLKNQSLRIGDESEFNLRKFRESIYTKMQQFGNSEQQQIYVKGTAPSITKKPTMNQSLGDIPSFDEICKKLQVRKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RZ36</accession>
    <name>A0A8S1RZ36_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0020312</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RZ36"/>
    <dbReference type="OrthoDB" id="298090at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8133256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="295" mass="34818" checksum="86F2A76955C262BF" modified="2022-10-12" version="1">MLNQENYKIQQFLVHLFNVDTSYSTCFHLTEPMLYQVFIEQIHQEDQRQDFTSFVEQYQMIYRKEFRPLSTRTHLHQQSLDNVKPFTYPSNSYVWIKAKQNKLKQQLTEQTSKEKSISTQKQSRQNQYIVYESLQLNTPKQIKNTLLITSPRIFKKTSNSMTFDLNSRNRNQENDQKQSLKTPQFKQPRFSESKKGNSVERQKDSLKFSNKTKITKETPSQSHIQRLRSLSGTEKLAAHQLIIGTINEINQLVHKMEEPKLDRIQSEESPSLQVPSRIGIMPAKLISIDVKKYKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S701</accession>
    <name>A0A8S1S701_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0040252</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S701"/>
    <dbReference type="OrthoDB" id="422186at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097320">
        <property type="term" value="P:plasma membrane tubulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45827">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45827:SF1">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="1">
        <location>
            <begin position="93"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8135495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="500" mass="59520" checksum="4287C9982360669C" modified="2022-10-12" version="1">MSLEEKQQFLVDEIINKAYDSEDFTKYMDRKKENGGQDLDIWQMDELRQAVYDYQKMKNAIMQIVDDDVGFKKKIDCQKLNGTEIGNTNNVYITIDYFDKKDTGFFSLSKSYVNYRIVTQPFQWAVTRRYSDFEWLREILTKQYPGVFVPPIANKTPTRQFSDAYLVKRMKFLQKFLNHLLNSTILKNDKYFCEFLRMQDEKEFKSLQTASEKVQKTTKLDKVISETGTIEVAFNPQTDNYIKAAGNLMTSLNLDFDVIMKQSKKMLQDFEMVSATMFQMGESFEVLTNHINQFNSSVQEPEKILKFEAVTITLNNMMMIWGRNFQNYVNYIQDNFRNFFKYHDKEIVQLKEHLLLRQQSQAEYLKYKERLDLKKEKFYQLKEFNKWEVSKEILDELKLNIENKKYCLSVMLPKETSQQNDLRDTYAYYNLSAYNEIKRVFDQNIDIYAKHFIKFADSQANNLTKMHVTWADIQGNLQGLDLITQNDQKVQIMQQPKPKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S9K8</accession>
    <name>A0A8S1S9K8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0050425</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S9K8"/>
    <dbReference type="OrthoDB" id="409924at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000001">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000018">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF18">
        <property type="entry name" value="PROTEIN TIS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="30"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="69"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="30"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="69"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8136946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="161" mass="19362" checksum="D76137B5B4EB1882" modified="2022-10-12" version="1">MSSACQDSSTRGIFQKVKDKNNYDQVIEEKKKTEICKNFLFKGSCKYQENCSFAHGDNELRDRVPANENFKTKPCKNYHKFGTCSYGLRCQYLHSEIKDKIKWWGYLQKQFKKNMVPFTFPWDKQDKTSQIQRIDILRFQRVFKDYQEVEQQLSYFKNLCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SC10</accession>
    <name>A0A8S1SC10_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0060221</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SC10"/>
    <dbReference type="OrthoDB" id="304247at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="437"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="147"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="438"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8137696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="460" mass="53604" checksum="A97FC1D07493CEB2" modified="2022-10-12" version="1">MNQEIFKSDNQKVSQLQGASSGKNLFTSETKQIITQQPVIEYSQQRTLIQSQNEANLKQTQQMPPQRIQSQNVLRQNMQIPQVSYPGTYSVQQNCQQPPPLCMNIVVVSKEEIETPWRLECEYLQSIIADLERRKEAKIVEKVVIEKVTDNTRVEQLESQLRSLRQENEYLKSQMIQMKNNYESQLENLRGDISLHSANAADANSMQAEFFAMRTQLEDQIARLRRQLADLENLYAQSQSDNDRLKQSNQNKDAEIQQLRLQLSKLQTSAQDTSQVRDLENQLRQAREEISRLERQLQQANQDIMNWKSKYTTIEQESYSKNNELIMQLRDLENKLAMITVEMGRLQLQLQNKDSELEDWKYRYTSLESQGTTVIQEKVTYLSQEVEVWKQKFIKSNHEYNKCQEELTMCQAELESLRKSGQKKEVVVTQRTVTSRTGNTLSVTGTTGSQSQIRRTGQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SC52</accession>
    <name>A0A8S1SC52_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0050133</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SC52"/>
    <dbReference type="OrthoDB" id="296750at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="127"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8136404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="520" mass="61026" checksum="2AFE029011147535" modified="2022-10-12" version="1">MTMTTVGYGDVVAVNEIEMIVASIVMFLSSCAFAYTMSSIGIILKNIYDTQLNYKKNLIQITQFMLKNNVDEQIQGRIRNYLNSQLYQEKKENMDDVNNILNSLPFNLQQDLNADIQARVIKQIKLIINHFSKSTQIQVAKNLQLISLLAGDVVYKQGDLQEDSLYFIHKGEVKQTEFQTKTTLKTLQKNQYLGIYSFFTGFCPKETAICNSPTQLYKINRKKFLEIIRSNQKDHEIFNHIKDKIIFTNNLVLFDHKCNFCNRPFHLEIDCPLIQYKPDLELILKKENFAEEHNYRISKKRKDNKYNTLGQLSKLNQSLNQFQEDQQIQLFGLNENNQNETPHQRTGFSEAVYQNSAQMIGQNAQNLSGISDEEQNSEMQHTPQNQSKQLIQQQILKNHHRSSLSKIKFTKLEQRASKSNFAPITDHQSGSFSILDEVKHQLYYQNTFNIDKVNFLSISYMPQYSLESQLKQMAKTMKRKNQRLQRLHDKFDKYTFFHQVKQLSIKLRVYYKRSNKNTFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJI5</accession>
    <name>A0A8S1SJI5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0070163</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJI5"/>
    <dbReference type="OrthoDB" id="285268at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000118">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000121">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF149">
        <property type="entry name" value="GH01077P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="9">
        <location>
            <begin position="81"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="10">
        <location>
            <begin position="232"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8138534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="516" mass="58046" checksum="9998F33F44AB82E5" modified="2022-10-12" version="1">MFLRNRLHSLRKLAQFCTAESVSGKKTPTTQAQQTKVERNYQLQFSKSALVDFGELPTGEIPEALKYDRPQALTQLDSGLRVVSEQYNSPLASITVAVKAGSRFETLESSGVSNFISKLNLRGTTTKSREQVEAEIDYLGGSLKVKQGRELQTYTLTFLPSELERAVNFLGDILTNSLYSPAQIEAEREGIYRESVSINDQYRVVAEAAHYTNYRDHYMGQPAAGIRDNIPNITEEQIRQFHKANFVAPNVVVSAAGNVNHEDFVSAVNKAFKGLGTSVPTEVPNSEKPYATPSIMLMKDDELTNLNVGVFFDTPGWNHPDVFALHYFQRLIGDYRADKHTGFHLNAPSRQYNTMHSLLGGLPDVTYQRCVYYAYSDTGLFGNYLIGNEVFATQMAYVSQMVLSDYASSVGQVEVFRARAKVFNELLSQESSAKQSREIAQQVFYWGRRVPRSEFARRISALDAGHLTRVATRHFWDKDISVVVWGPTHLLDAVAHYNRSWKRSTLGGYAQPYYEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJP8</accession>
    <name>A0A8S1SJP8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0080446</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8140088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJP8"/>
    <dbReference type="OrthoDB" id="304405at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051581">
        <property type="entry name" value="Ca-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34524">
        <property type="entry name" value="CALCYPHOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34524:SF6">
        <property type="entry name" value="CALCYPHOSINE LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="71"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="194"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="233"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8140088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="588" mass="71859" checksum="FE4CA9C2D6E8D599" modified="2022-10-12" version="1">MYLSQKTQLRLANLITTIAEGEKKTEIFRQVLAEYNMFEPYTAFKRLDYKKTGQLTVSDLVNFLLENKINASNSQAEYLFKKLDQNKDGRITYPDFIQFILPKEDSRLRHIVSQREYSSNKINKQLPTEVEWGLSKVFYQEIKNYISISTAQEILTGCADFTSLDAFRCIDQAFLGYITIETLQNFLKQNGIRLSFDDLIAFFRVVDQDADGKINYSELLEAITFTPDYFQQERQFVDELRKSREKIIQLEKEREEINNLKRSRERIEELKKSREYLEELRKSREKVEQLEELRQSSERQEDLKKSRERLEQLKNSRERMEKLKYSSEKVQDFKNSRESLEDLKKSREILQELKSSRERLNQLEELKKSRQKIEELEELKKSRERQAQIAELRQSRERLEDLRKSREKLQQMEYERKNYDKMEELKKSRERIEELQKQRKKMEELRNYQESLELQKKERDGMDLRRSQERIQELKKSRQRIDQMEALRNSREQERTIQEMRQSRERIEQLRDSELRIQQLENQRRSLERSRQLEKIERDLEYERQRSQERIQRLELEAEIERKERERALEEEINFVQSRIYDTPKRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJR7</accession>
    <name>A0A8S1SJR7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0080178</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJR7"/>
    <dbReference type="OrthoDB" id="284090at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="23"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8139597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="152" mass="17736" checksum="7A9A8E887D17EEFD" modified="2022-10-12" version="1">MGNTQLTDQQILLYKNETQFTPAQIRRIHKKFQQLDTNNKGFVSATDFIKIIQHHKYSPLLIQQLIGRKQQEIDFIQLVRLLNAIQFGDKNTLLTRIMDQDRDGLIGTEDIQKTITVLNPSCFKNSYELAQQVVNDNEKLTYNKMIKLLIDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SNM9</accession>
    <name>A0A8S1SNM9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome biogenesis protein NOP53</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0100159</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NOP53 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SNM9"/>
    <dbReference type="OrthoDB" id="5072at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011687">
        <property type="entry name" value="Nop53/GLTSCR2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14211">
        <property type="entry name" value="GLIOMA SUPPRESSOR CANDIDATE REGION GENE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14211:SF7">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN NOP53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07767">
        <property type="entry name" value="Nop53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017302">
        <property type="entry name" value="Gltscr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8141510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="316" mass="37627" checksum="E0F3C7C10364B366" modified="2022-10-12" version="1">MVNIKKKNLRARQGTQRWRKNIIVEMGEFENIDFTQKDAQPMDFQVDAQPINIYENKDRFKKKLIDPKKQSQQEKIKIQNMVEQQKNQQQTQQQNEFKDIWSEEGKLKKVLRTNVKAIVTPHPGQSYNPQLKDYNELLDDVVKKEIRSHQRLQNQETEEEKAQKLIQREREKKKQKKMSKIQKIHGKEVVDRLIEKKKNHELLLIKKIKKELNAEAKLRLQKRIQQRKEKLLLERARKLQGRNLVPIKIGNKKIDVTQDEFVLDDQLKSHLRHVSQGDLLVKSEFDGFVRRGLIEPKSKSNKLSKAKVPLFKIKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SP13</accession>
    <name>A0A8S1SP13_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">V-ATPase proteolipid subunit C-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0110174</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the V-ATPase proteolipid subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SP13"/>
    <dbReference type="OrthoDB" id="305104at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18175">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6C_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18176">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6C_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.610:FF:000001">
        <property type="entry name" value="V-type proton ATPase proteolipid subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011555">
        <property type="entry name" value="ATPase_proteolipid_su_C_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01100">
        <property type="entry name" value="V_ATP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10263">
        <property type="entry name" value="V-TYPE PROTON ATPASE PROTEOLIPID SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="10">
        <location>
            <begin position="25"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="10">
        <location>
            <begin position="103"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8142475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="165" mass="17064" checksum="619A904EEEDFDDB0" modified="2022-10-12" version="1">MLFILDTMVSSDDTPISASSFFGFMGVTMALVLANLGAGYGTFKAGAGIAAIGIWKPEIIMKSLIPVVMAGILGIYGMIVAVLLSQKVKSPLTYSYKSGFAHMASGLCCGCSCIAAGFAIGIVGDVGVRGNAQQERLFVGLILILIFAEALALYGLIVSLILSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SPC5</accession>
    <name>A0A8S1SPC5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LisH domain-containing protein ARMC9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0100298</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SPC5"/>
    <dbReference type="OrthoDB" id="293022at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097542">
        <property type="term" value="C:ciliary tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040369">
        <property type="entry name" value="ARMC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048957">
        <property type="entry name" value="ARMC9_LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14881">
        <property type="entry name" value="LISH DOMAIN-CONTAINING PROTEIN ARMC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14881:SF4">
        <property type="entry name" value="LISH DOMAIN-CONTAINING PROTEIN ARMC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21051">
        <property type="entry name" value="ARMC9_LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8141770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="482" mass="56226" checksum="5C345EB6B110E814" modified="2022-10-12" version="1">MRTANSNQGQSIVPDTNEINDYIKEYLRYSNYSNTLECFDAEIKSKQVSNKMLNKQQVIKQTGDDMPRIFQLLKSDNIKTKREINLEKEQKQFNKKYQQILQAGRQIFSVSINLLQLLHSLKETAKNENLSETLENYKIQLGKYHKVIINEGKPEGTELITEQVMHEHKTKLFKNYQDKHVDGMIEVLLSLRVNALQIAPELRKNLVYELIRNDVFNIEATDKFDFVVHLLDINNQSLRHAITSLISVISSTLRGVEYLTYNGNMIIIEKIIKILKEQENGSVTQRFCLAILQKASIKDTVIPSYVQNEMIQWIINLIQKSVNSKIHVFCLDFASATLANIIHTPYTLQYLEHQPRFAHQVMEQLLKFIKDQIQVSVLMHILICLSYLSKENFAKQMEECRFVDRISQFVEFYSVINTENEAAEIDKKTVLDLCAHMFHPKDTSLDNSETLELNELKTEDRIREYENEQGELIFECFQDEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SSL4</accession>
    <name>A0A8S1SSL4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0110399</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SSL4"/>
    <dbReference type="OrthoDB" id="308641at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="439"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8142910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="516" mass="62019" checksum="E1211193BEE982A7" modified="2022-10-12" version="1">MINQKSLSILQLIPENFQRSSKSLTTIIKKQINEDEKQVRPIKFLYVNKEKKNKLNIGQVEKYKQLLRTGASSFIFYIYKNKDCQENYFNSLQRTEIKVPLVDTTLQIGIYVWDHIHYIDQKSINVYQFQCILGTQEDENNQLLFVVSQLASKIVVIIENYNQEEIEIIQQLKAHGYKDQIDVFNLIFTIKIPIYYNSEEVRYIDIYENNLWEKVYVYKKSSKDTMRLWQEETLMSLTKKTYKDIEVDILTVLSVVNYLKQLNFSEENCDLIFLNAIKKQIEYSKKDIQEQYIQEIQKLCSNSNHFQQNQLLLTLNQVRNNAQSQYYQQLSQFLNLEEYQRQLIELSQFINNIEEDCLNLFHDVAHYDLLQRMKHNIKSSQNNDTFLNHYQNFLSMLRNFDDIMGVKYKYLKRHFKKKYNKLLHFYILSTFEREQIDFIEQIELDIEQLQITVDQLQNEVEEFEYKIKISQSKHHTRQSSLQTTVGMAQSQHYESLQKLNQKVETLQSQAHVWIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1STW1</accession>
    <name>A0A8S1STW1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0110356</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1STW1"/>
    <dbReference type="OrthoDB" id="289240at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45949">
        <property type="entry name" value="SORTING NEXIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45949:SF2">
        <property type="entry name" value="SORTING NEXIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="4">
        <location>
            <begin position="30"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8142826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="419" mass="49548" checksum="1C29CAAD5D3EC7FF" modified="2022-10-12" version="1">MDNELIDIPIDYDEPKLESQESYQGSQEAVSQIISATPTRNQSEINQKQSIHTITFHLTEQVQQSYTQYYENISYVLWSQINDEPAQTVNRKYTHFEWLNGALKREFPGLLIPAIPPKSVLAKINLTEHTSLVREKRYRQLEEFLNKIKDHPQLQVSNKFQIFLTGSSEIMQQELLELSQYQTYAHQLGSAIANAGSKAYDIAKGSFNYLSSFIYTGQQNSQKQLQYPELEIQQDIKECFESWEDLLIEEQQKVKRIFEQYQNIVNIKQDQNEDTKKVISSLKELDLSSLEKDINKTEASCYLNESILNEIKLSVIFKLELCILDLDEAIGTIKRRSELIYQIQQQIKMHNQIESFNEKIQYKEQIRENQKLLKIIETNFSTDIKIFANWLNKYLEQLIRYYNEQFQKLYREQNKNWND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SV73</accession>
    <name>A0A8S1SV73_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0140306</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SV73"/>
    <dbReference type="OrthoDB" id="304927at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8145561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="69" mass="7614" checksum="D2BE423231E82F9D" modified="2022-10-12" version="1">MGCSLRKSYNPNLENKRHSIITHTLTPDKKPSLDVFNPILGKVVSVPILVPASKSSIVMRRSTQLTIGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SVG8</accession>
    <name>A0A8S1SVG8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0140331</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SVG8"/>
    <dbReference type="OrthoDB" id="310806at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013111">
        <property type="entry name" value="EGF_extracell"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093:SF18">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07974">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035744952" description="EGF-like domain-containing protein" evidence="5">
        <location>
            <begin position="17"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="449"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="527"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="453"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="471"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="531"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="550"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8145611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="597" mass="66504" checksum="5681FDE7F0B7F912" modified="2022-10-12" version="1">MLIYILLLWLINTKNGPVLIEKFGRSNKKQVESPPPDFECIDELDEDDTTPKEYPLKIGSLYHLSDGNWYLRSEDSTNQQVTLINMNDISRIKFTEKEIEATLPDKQPEQKLKDMEWTPLLAFERFPRQRGGHSMHSIGDYVVLFGGCLLNIQCFNDLFLFNARTRIWTTPKVFGIPPVGRSGFGSLVNGARLYIFGGHTLQGLVNDLFVFDLESRSWNQLSWPGQAPTPRAGHKMVLTKLGGLIFGGFVGEVYSNDIYILDFVNERWGKPSGGGDVPLGRESFSMTYHHGLTYVFGGYAKGMIMNDLYTINEDLIWQKREVEGDIPSPRQGAAMAEYDNRIFIVGGCNPILFECYNDVYTLDTESMSFTNVTVEKKRNLRQVAYSSMVFAGSLLIHFGGCKLLQYCSDSLIGLAVASSETCPPCKNDGECRAGHCSCKQGWQGIDCTQQVLCKQNCLEQGICLSNGYCKCYPGYTGSICQLNVPCPGNCTDEEHGICELDGKCKCFEGFSGTTCSGNPEVDSKLPQSTGCLDECNHRGQCNYETGLCACEDGFSGPDCSLVQGTESGYTSIWDLLKDYDGEHENDLDTIRRTNANL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SVL8</accession>
    <name>A0A8S1SVL8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0120123</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SVL8"/>
    <dbReference type="OrthoDB" id="1305878at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23327">
        <property type="entry name" value="RING FINGER PROTEIN 127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23327:SF51">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR OF YEAST FORM ADHERENCE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="40"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8143267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="241" mass="28639" checksum="9FFB48DF5E84B670" modified="2022-10-12" version="1">MNKQQKKIQKQNEKLKIQSQDNDSQQNIEDIIHDKNDFICPICLNYIVAAVSLKCGHTFCEICLHEYLLYFKGCHICNDNMRKSKFAYCYLLDQMIHEFIKSHHPEELKTYEMDKIKNKEWRKKKQVSSIDVGQQIDVRDPNFVWNVGTIKRLKISQEASKIKYLVIHYEGKSDKHDEEIAENSPRFAALGFYTSRNDIPKYYKQTKNPFLKNLLYIECMDPNDNQFNQQFFIEDNSSESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SXR9</accession>
    <name>A0A8S1SXR9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0150006</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8145805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SXR9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8145805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="104" mass="12684" checksum="3B42A430A02624E0" modified="2022-10-12" version="1">MTEFHKIYWCQPIWQQQFSLDQNLLIWQIDKSKIFILNLEKISKQKQLPFQSDSKIQSRTIILSTKFLIRSNPLESYSLNIWSIENNKKQLIYIVMKKILKFYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T0E4</accession>
    <name>A0A8S1T0E4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPENT_87.1.T0150114</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8145980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T0E4"/>
    <dbReference type="OrthoDB" id="9984778at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021319">
        <property type="entry name" value="DUF2921"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052788">
        <property type="entry name" value="RING-type_E3_ligase_ATL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024766">
        <property type="entry name" value="Znf_RING_H2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45798:SF97">
        <property type="entry name" value="ALCOHOL-SENSITIVE RING FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45798">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL61-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11145">
        <property type="entry name" value="DUF2921"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12678">
        <property type="entry name" value="zf-rbx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035794494" description="RING-type E3 ubiquitin transferase" evidence="15">
        <location>
            <begin position="23"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="246"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="290"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="343"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="373"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="425"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8145980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="489" mass="58247" checksum="D0CF4B90FECA20A1" modified="2022-10-12" version="1">MELLQKFISLYFLLLYLPQVNLQNVNSTEQLMLLLNKELFSGKWWSNNPSIYQFNYLTLDSGMIKVKFVGNSIQFVALNPKYYEDKHVYGEFLLTNYSESSQAWIGESKLLLEGSYSRSQPWIESFCNGTYIVKLNQKLENLDLNQLQIDVDLKSNPTFLHCQIDMSFNVKRVYDFSFINNLIFCMMATMILTVQYLLSKQLLDQLKYRLIEVEQMSRLTLFFSLFQGLSLFYLFVEILFQNFYNYYLFILPTFIQFLQIFKNQAIFGLVWSQRLQNAFVERREYKKKLVIMYLIIWITTIVNMILFNIFGQSFYYMFTQAFILYPQIIHNLRQGNKYQFNKIYIFGLLSSKFFFYLYLRSCPVNVLGLKPDYLFVGEFIAIYFFSLLLIILQSKYGSRCLIPKCLKPKKFQFLQKVKVNNEMECPICLNHLYLNPIDTDDKPLEKSLICQIMVTPCQHSFHQQCLESWINVQKNCPICRKIILRLDDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T2H5</accession>
    <name>A0A8S1T2H5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Leucine Rich Repeat family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0140350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052394">
        <property type="entry name" value="LRR-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="5"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8145647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1698" mass="201513" checksum="F8631E6E9755B048" modified="2022-10-12" version="1">MLFKKLKIYNTKEKLLERLKELKKEEEIKRNTPIKEKVKRTFSTSQSQRTTLNSLNRLSTSPKVQCILEIEKIKMPLTFERYQNITRMTEIPIRQQISTPQPPQKSILERLNEQEEEKMIRELIDNYKPEQFDDMMENDQQEIERQNDEAHTNSMKQFSYQDYSPQRMNLIKKHQHHHHKSNPSHLKNNQRYDEQKQLYLQQLEYKGDVNQEFNTDRGKYMRKFRLANKNLNRISQEDYRKTIKQFNKLEDYAQFYKQSPNLYMQLNKSSHQQQIFQSGLGLVQQCNHIQAANAQAQIRSPQQVKVFSDALKTHQCKTLTHLKLNHNKLNSLKIQQLTKSFPQHLQELDLMNNGLDSKSCQLLSKYIQKSQIKKVNLENNRIGDIGSNALCQAVQDHDYLLYLNLSKNNLTEHCCVELANYLKKTQVLFELYLHFNSINSIGAVNIWKALYKNSSVKVFDISYNRTASLECSQQMAKVIIKQYPELMHIDISYNGFNEEQSIEIKKALDQNQNIYGFHYQGNCQKYSVDSTGHLRDRIQEAIDIQKKIEEYKKNPQTTLKLVDDNLNPEIQLLEQKQHQQYQQKRIKDLQTDAELYRNFRFRRIQGMKPIKQNYDTDSELDACWICDGWQEVKFTWTPGKSGGMNNDPIFLHLNFENYKPVLMTLHNGEYSAYKMCPPNFRVTYFYSNPVLGIQTTAKNQLITQTPQDDPLYSNKQQFLYNGDIFIEGNKMGFVNELFTDDKQSIMDRYFAKIFSKPREEEKTFDLTQFLTKTEKFWSYEISIFKNYQPDNEEQIDECFEYDYGSSKINKIIKDPIEYNEVKEIMREYYPCIFASYKFLASTLIGATIPCISSNAFSDFLSATGVISEKFRSGDIDLNFIATSNVKDINYPNVYEKALVRYQLMEVLVRIAIDKYLRTQICKTIKESLRKMFEEDGIKLKLQEIDRSQDWRDMRYWNEQCDILLSYLCQNCFSXYPELMHIDISYNGFNEEQSIEIKKALDQNQNIYGFHYQGNCQKYSVDSTGHLRDRIQEAIDIQKKIEEYKKNPQTTLKLVDDNLNPEIQLLEQKQHQQYQQKRIKDLQTDAELYRNFRFRRIQGMKPIKQNYDTDSELDACWICDGWQEVKFTWTPGKSGGMNNDPIFLHLNFENYKPVLMTLHNGEYSAYKMCPPNFRVTYFYSNPVLGIQTTAKNQLITQTPQDDPLYSNKQQFLYNGDIFIEGNKMGFVNELFTDDKQSIMDRYFAKIFSKPREEEKTFDLTQFLTKTEKFWSYEISIFKNYQPDNEEQIDECFEYDYGSSKINKIIKDPIEYNEVKEIMREYYPCIFASYKFLASTLIGATIPCISSNAFSDFLSATGVISEKFRSGDIDLNFIATSNVKDINYPNVYEKALVRYQLMEVLVRIAIDKYLRTQICKTIKESLRKMFEEDGIKLKLQEIDRSQDWRDMRYWNEQCDILLKDRLPMLKLLFKYTSKLNSKQKYYKHIWLQFKDFRDLLNKCDLYCDIFVERDAYLAYLLAMQTSVDELYLLKHFQMEFYEFLEALARCAEKLSLIRTNDQITIDDRRQQPLFKKLDALTLLIYIRLGEIVKAQFRESDDLSEFDKCMQKTYGQVVKQPNTDLEEDNNEKITPQQEEKILEEQLKTIVVQPILTMPQPNKRSTGMTFLQVIKQAQQQKQLKNTFNLTNVVQYFRNKEEEQLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T2J3</accession>
    <name>A0A8S1T2J3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0140398</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T2J3"/>
    <dbReference type="OrthoDB" id="63070at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="290"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="320"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8145726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="373" mass="46166" checksum="A4A03E18F8A8959E" modified="2022-10-12" version="1">MEFQKWKNQVFIDFLWTQQMDLVFIIQQEIKFLYFMFWRQKFRCWKNQNFNDWISLEPYKKHTDWVMCLVMNKNEIYSFLEVVIIQQKYGSLILIKMNQHFFIHQINIIIMLFRQVQINQRIISIMCIREELDYYLGKKRKNTYEFKYFVRIPFIYKQSIYNYRLKDSFITGNSFISTTGDQGIDMIYFFELIEGIYQEIQHKSIQLMNNDQDYDEYFFSIIYNKQRNLILVRHKSQIYLIRNINNGNFKIVDQLNCNTSDIYWTITDNGQYFVFWIVKIMDFQFMNYKVNENSLIFIYIEIYIFLMTPSNYEKQLHQQVFMALFISTYKIYIQILIFTSFNYLNLRIQNQVSNIDFILLFELEKYCNSNRFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T9E3</accession>
    <name>A0A8S1T9E3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0170217</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T9E3"/>
    <dbReference type="OrthoDB" id="285644at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8147936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="148" mass="17805" checksum="1675EB4CBFAA13A7" modified="2022-10-12" version="1">MSEYFMQFQKLQKRIQLREKASDEAWQVLQGEERQNLLNGINFYNYRFFALRTFPIAIIGILLMTPVLQTKSRFFVKELSLIFGFSGGIIYGDYQNSEYFWNNYGKIIMENTNYNDSGLTQDQMEKMRKLYEKSKNYRPDNKTDKMYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TAD3</accession>
    <name>A0A8S1TAD3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0180135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TAD3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8148577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="25570" checksum="236A09E402DF350F" modified="2022-10-12" version="1">MEINKPFIITKRQGDSLLPPDCEEFNEAIEDKIRFLQSSNIFIICAFIYQCIRFLYYFIHYRTICYTYAILCLVIVGGNIFWIASSTYGFLGINSRSSSEIGKYLCLTSVVLAIRLITYFLVWALMQGYAQQYNILSKTRLLQENTTQQPTPTEITDDQKQEIVCGQLYQGTLMMVQAIIETIFLGVIIYFVVQVKKYITNYESNQKKKQRTWNLQASIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TAZ9</accession>
    <name>A0A8S1TAZ9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0170169</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TAZ9"/>
    <dbReference type="OrthoDB" id="307685at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="169"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8147842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="406" mass="48703" checksum="1B908FF2471531E7" modified="2022-10-12" version="1">MSSKDKDKKKKTAKLEKKDTKILSIPDLDQSQLIARVISRGGDKETKNIANNEDEMQTFTQSHSNGVAQMNDFQGIQSQQQFSTNFLRNNDYNTNKFMSDSTYEEYHGQLNKTPYYQQQTLSMQNYAQQYPQQYYHMGNQDQIDYRYEKIRNQINLSLLPKRQEILYQIQKIENRIDEIKYQTQQIENMTREECDFIIDRLRSAENQKLQRLYQDKDELISNIEQIDSLQSYALNNQEQIPQSKILENIERIANQRIKTQIDINSNDLPQEFIQLKQSYQQNIIQQQLIEFKNEVIWKLVNDSKIEIQKIQADLEQQVYQEYQKWLNNEEIYKQELNKYKLQCTFCGVNFDYKQINTNCQSNIQNAKLQFECEENPQEQYIGTYRHFFSKSKELQESQMQIQNKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TCD1</accession>
    <name>A0A8S1TCD1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">T-complex protein 1 subunit eta</fullName>
            <shortName evidence="9">TCP-1-eta</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">CCT-eta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0180166</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="8 9">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterooligomeric complex that forms two stacked rings.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TCD1"/>
    <dbReference type="OrthoDB" id="1935484at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03340">
        <property type="entry name" value="TCP1_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000017">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.260.10:FF:000022">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000006">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012720">
        <property type="entry name" value="Chap_CCT_eta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02345">
        <property type="entry name" value="chap_CCT_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="529"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="529"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="541"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8148639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="553" mass="60894" checksum="EA9D9BAA50E433A1" modified="2022-10-12" version="1">MSQFLQPTILLLREGTDTSQGRAQIVSNINAVQAVADVVKSTLGPRGMDKMIQTGPKVTISNDGATILNLLEVVHPAARVLVDIAKSQDDEVGDGTTSTTLLAAELLKEAKPFIEEGIHPQIVIQGYRKALELALEKLEGFSINISRDSPEDKRDTLLKCAQTALNSKLLANTKQFFSELVVSAVEKLDPNILDRDLIGIKNVTGGSVTDSFLVDGVAFKKTFSYAGFEQQPKRFENPKICLLNLELELKSEKENAEIRIEKPEEYQQIVDAEWALIYEKLEIIVKAGAQIVLSKLPIGDLATQYFADRNIFCAGRVPQEDLLRVQKATGGQVQTTVNGLNPETFGTCGLFEEVQVGAERYNLFKNCPQSRTATIVLRGGAEQFIQEAERSLNDAIMIVRRCFKANKIVAGGGAIELELSRYLRHYARSVTGKTQYIVNSYAKALEVIPRTIAENAGLNSIEIMNKLRQRHAQGGEEGRWYGVDINGQSGVCDTHSSFVWEPSLVKRNALCSATEAACAILSIDETVKNPKSDQDTKMKPRGQPGRPPMGMRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TEQ6</accession>
    <name>A0A8S1TEQ6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0190230</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TEQ6"/>
    <dbReference type="OrthoDB" id="284015at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8149676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="97" mass="11658" checksum="22D9E4BEB99C6C37" modified="2022-10-12" version="1">MMRIQKCTGDEEIELEKDINLWFERMYTEISAKQQETKLRTDKYNVEIKQKIKKHKICFRDEINPEVGLVDVNVVENWKAYNINQRNPVDDCFCQTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TGZ8</accession>
    <name>A0A8S1TGZ8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0240187</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TGZ8"/>
    <dbReference type="OrthoDB" id="5590282at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000057">
        <property type="entry name" value="Cyclin N-terminal domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000326">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0195">Cyclin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="3">
        <location>
            <begin position="146"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="37"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8153401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="405" mass="47859" checksum="248C90B28C2354A3" modified="2022-10-12" version="1">MKQPLQSRLIIQDARRKNLSAVALLDPSPKQITIRKASIGGLKNKENMNSNDTQRKQKSSQNTNTEDIRKQISQLIKKTAQSQRTKLKKIDIGCQEQIEINDQKEGIFDEITLRLLYQKELEYKIHPQFFEHQCHISPLMRSILYDWISEVCKEFTLKRETFHLCIHNLDRYMSKISITKSELQLCGLASLLIACKIEEIYPPKVNDFSSASNYGFTEQQILDKEQDILNELKWMINPPTLYLWSSWYLSQWDIYYPQTNLQMKQPTQSSYTLFRHFMTFLDCAILDIKLYQFTNREIVSSLLYLVLLKQYCGCTYQKIVENKIQEKDILDFQRIYKPFIEIVFGFQFTQLNKCIRYLTKYLILDIVVDQPGTTKVVAEKELVEVYEYFLSIQTHNPAGLQFIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TIM2</accession>
    <name>A0A8S1TIM2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0210376</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TIM2"/>
    <dbReference type="OrthoDB" id="299008at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="728"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="831"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1037"/>
            <end position="1060"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8151484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1103" mass="130552" checksum="4D6F9EB13E5A83C5" modified="2022-10-12" version="1">MDDIKTMIIQQAQQKVNYYQKLNQDSSFNLNNILQITRDTCNQIIQTNKILKSKFSDYPCLSIQNAICYYESEITNNYYEARKVSNQTSMSDEKFLMFNSSLISNKDKIGYLLIEISNDFQQTEIKFASNQFQKLFERKNYDQKLQLDDLLPKFLIKQHPTFIQNFLTTGHSKFFQNFQLNFLNTNDGLIKGVGMCYDLTKIEKQQDQLIFAVFFQELSDSKAYVLVDGITKYSYFSECFLKKLGFQEHEIKFIKSSNILSNLSISYLLPDYDTLIDINEQEIRLQGVDLYFANIFRLFSTIFTEKKVQNSQNTFTKIAWHRKDKLFKYSVNLQITRRQFNELYYIIIEILSIEANSQTNIIDSINRIECQNYLQKNECSISEESESYSSGQINLQYQDVPNKVEIFQGLKNEFILSHHQLDSLSQPRITICEPGRIVTPNESSSDTKRKFHSRNQQQFYSQKLSQADIQALISEKKLDIPYDDEQQQQLKMDQLSDKSSIEDEQKKKFMKKFELVQKLMVYKRPLKLIEILSLLTLQNIIFTIFSLIAITVMFNDLSQVLKEIDTISLHSSIMVKHDIFFSIRATIKIYQEYQKKKVLLNVTNLISPFYNNLAESYLDYQNDLYNQFQNLNLQPYLDDYYLIIYQLESLSNQQLQEKNVTVREALMINLQYEFAVLDAFLNQKDISNSTFQVFLFTNFVKLHQYLEDLTKNLLSESTNRSLSVASKWMVIWIFFQIGALVLAIFNFYCYYNYITFYDKFLLALKFIDSELIAQEIERYKEMIKKLKDNSRHVFEYKIDLDQIQQSKTNKRQLSIKNKEIINKKVIKLRRFSSFMLIFVLNLIFGGYSAIINQRTQIYLNKYEKTANLFKLFQDLSLSGGNCFLYKEILLGFNNYTFYFQEDRGNMYKMLYNSLDSIQQYLNLSNTLDFNTYLATDSFIILFNDLKTNSLCQYFTQEYLYPYCQLSFDGVLSSGLVSSLNYIQNTIKTQQAINNFTFKTDYNLYEQEGSQIITRAFLNMSLTFKEDLTSLTQNQTDLAYNLSIAFFIYSFIVTFCLLFYLKYHLTNEYYIIRRIISLIPINVLMFDETLDRYLREIALHQELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TMR1</accession>
    <name>A0A8S1TMR1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0270017</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10035969at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332:SF175">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="26"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="31"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035803631" description="EGF-like domain-containing protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="4580"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="4445"/>
            <end position="4466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="4478"/>
            <end position="4502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="4514"/>
            <end position="4541"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="174"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="366"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="476"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="531"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="900"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="962"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="1059"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="1106"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="1260"/>
            <end position="1305"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="1306"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="1361"/>
            <end position="1403"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="1408"/>
            <end position="1452"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="1542"/>
            <end position="1589"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="1597"/>
            <end position="1635"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="1643"/>
            <end position="1684"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="1692"/>
            <end position="1732"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="1740"/>
            <end position="1779"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="1787"/>
            <end position="1826"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="1834"/>
            <end position="1874"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="2362"/>
            <end position="2401"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="2409"/>
            <end position="2449"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="2450"/>
            <end position="2498"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="2499"/>
            <end position="2548"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="2556"/>
            <end position="2593"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="2594"/>
            <end position="2622"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="2644"/>
            <end position="2681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8155301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="4580" mass="522628" checksum="DC28F1FFE6BF5186" modified="2022-10-12" version="1">[encoded data, 4580 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TPJ9</accession>
    <name>A0A8S1TPJ9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0250051</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8153828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TPJ9"/>
    <dbReference type="OrthoDB" id="303865at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000894">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26200">
        <property type="entry name" value="Rcat_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="120"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="46"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8153828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="469" mass="56428" checksum="BF735E47A5906997" modified="2022-10-12" version="1">MGQICMRNCCNERGPDEDFCIMLPNIFSSNSQIKSIQQEEGRKIILLMQSNLYEEIQSKLENEQNNTQESQQIVLNIFLYFNFKEEDVKRCFNLLKTNDDYKENLKLIGIYRESSLKYYKKQLCSLCHLENMECYSLRCQHIFCKDCWNLMIEIQLSNFIPIVKCLEYQCLERLPHQFLEQYQLYKEILVKRMLDNDKNYTWCPGLNCQNLYKQQGIQQKVKCHCGEQFCPYCKVESHYPISCEIFKEITQFKESNQSWTTLDISICPNCNRNIQKIEGCLQISCVCGNNFCNKCQQAWNKEHGQNYFNCSYASYNKNPSQILNQLRQRESTITKEIFQLQNYQQQFNQKQYDYYTRQFQMGLHNLIKFKKFEKIVQYFTYYLNEKFNDDQLDHYCDKYSLEINNYQRILLNQLQGVIINDAEMINNEKIGFIDFVNLISQMDQNLKIQKHCFKKYIIDIQLEKQQQLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TQ51</accession>
    <name>A0A8S1TQ51_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0240015</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TQ51"/>
    <dbReference type="OrthoDB" id="10069349at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23804">
        <property type="entry name" value="UBCc_UBE2S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000031">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="8">
        <location>
            <begin position="10"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8153069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="199" mass="23035" checksum="9A320657E79366F3" modified="2022-10-12" version="1">MIQEPQINPQLGPIIAKQLQMMIKQPIDGVHVIFNEQDVYDIQADIDGPVDTPFQGGVFRCKLILPPQFPQMAPKGLFNTKIFHPNVSEKGEICVNTLKKDWNPIQWSLKHIFEVIKCLLIVPFPESSLNEEAGKLFMENYDEYFKRAKLLTSIYALKQEANVLKGNNQIVFDEADKKNKVNQIQQKKENDQKKWLKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TR41</accession>
    <name>A0A8S1TR41_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0240195</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TR41"/>
    <dbReference type="OrthoDB" id="5979581at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000616">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="13"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8153409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="443" mass="51900" checksum="8BB0EECECB137286" modified="2022-10-12" version="1">MSQNSSKIYANRFIMKKKLSQGSFGMVYQVYDRATNSECALKVELEENQEKKTLEREITMLIQLENVEGIPQLIWYGFEGQTQLMAMQLLGKDLAYYLKLYKKFSFKCCLQVAYDCIDILKNIHKRFIIHRDLKPDNIMMSIDLNQIYIVDFGVSKIYFEADHIPFRQDKDFIGTLRYASIAAHKGQELGRKDDLESLFYVVFYFLRGNLPWQNIPVESSERNRVVGEMKQTISIKELTSIKCPELIKIFQYIRKLKFDEQPDYEFIKDLLVEAAKNNNIKLDGYYDWTEGMKLSKKLSESKVSMDKKEFQSPKQQMKSTTKISNQVIWSVCDNSKPQSQKNLLMPPEIKKRSDIHHFSSGSNISVVMGTYSSMRLRYLPSTVNKKSPGLKRMVSIEFVAHDESVYQEQIDENYEESPLNEKYKEYPNGICFKDNNKFNQIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U138</accession>
    <name>A0A8S1U138_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0310160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U138"/>
    <dbReference type="OrthoDB" id="286864at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021258">
        <property type="entry name" value="Epiplasmin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10992">
        <property type="entry name" value="Epiplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8158084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="284" mass="32812" checksum="FBA213B74B37A72F" modified="2022-10-12" version="1">MSNHPQSQQPQQQAQPPQPQARTVAPTYPNTVLPQAYPNAFLQPTYYPASPVRQSYVAPVQYAPMPQMSVAPIAQVPVQQYAVAPVAPVAQHQSIKGESRIEYVPYQKAVIEYEEQEIVSYVPRETKVTDYYAVEYQTEYIPQVFQEKYTEYVPVDRYQERVEYQAVERQVVHQQPTQYVQQAVSVVQQPYVQQSVQYVQQPVQYVQQPVQYVQQPMIASRMVPQFAQPLQQYPPQQYHPAPVTRPQQPSQQPHQSHQSSHQPQQVASQQPRSQPSQQQQSQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U4Z4</accession>
    <name>A0A8S1U4Z4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0350043</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U4Z4"/>
    <dbReference type="OrthoDB" id="10260455at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039879">
        <property type="entry name" value="EFC10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21847">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21847:SF1">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8160551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="142" mass="17007" checksum="3A724C87522AAE00" modified="2022-10-12" version="1">MASQLLEKLFQNNPHLNEIQKDRIAETLQYLEQHKISLLFEELLSQLIFEMSNQPRKTLLDALKNYKNQQFFDTSDYEVIFEHFDIFNDKTVKINSIRQGLQIIGITKSEEEINQKYQSILKKGFLNKNEFIDIMKNEYTQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U9G6</accession>
    <name>A0A8S1U9G6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0370037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8161811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U9G6"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035911006" evidence="1">
        <location>
            <begin position="18"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8161811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="294" mass="34171" checksum="228BCEE20E563C6F" modified="2022-10-12" version="1">MLQQKLIWFLNWQICIAITTCAKIVQKASCLSTCGYLQTDKCQVFDKYSDAFSTDQTTCFEDDGFLTDGIACLQSRNFIQEQSKQRWNLFLKRLIMQIEGMLIFYWNQTQTLQTSSYQLLYTDGAKCFSLEICSSYTELDATQERMVLVSTLIMLVRLQEPNYVDQSNVKTIILLVVVLYMVKNVFLIQRTVFPKAKHILEEDQLVKINFFAHSHLLIKLTELARPLHNELMQILALQQMKLTRVAKNLIWNYHILYYHICDTFAKNTDCQPVPSFDGQGFTVCTIKINKSVCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UC21</accession>
    <name>A0A8S1UC21_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0360224</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UC21"/>
    <dbReference type="OrthoDB" id="436852at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46364">
        <property type="entry name" value="OS08G0421900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BAH" evidence="6">
        <location>
            <begin position="43"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="5">
        <location>
            <begin position="173"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8161513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="223" mass="26277" checksum="C95B343395939A3D" modified="2022-10-12" version="1">MSEEEKVNQISPNTVNELVKNDKYGHLIQLYLKKDPTRIKKYNSIQKSNKIYHVNQDVAVCALNDDIYSAKLIKIYCIKDPSNTFIPIIQVQWYYSKQDLKIDQKLIKCISDKELFFSTHSEYLPANKIQVGIKVLTFEEYSDLEFEEETIFFSRAAIDLESMEPRPNIKLWKKSCVCQLPQNPDLQMIQCDECENWFHLDCVELQDQDITKIDKYLCPRCSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UH44</accession>
    <name>A0A8S1UH44_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0370143</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8161986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UH44"/>
    <dbReference type="OrthoDB" id="307806at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8161986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="211" mass="25097" checksum="474C08F9EEBB885A" modified="2022-10-12" version="1">MSKGDAKTLKEEIQKLYDPLLFYQHGDNPAYKRYIEIMQENAQEGKQIDPRTYFVNKLQRIHVARNIQDFVERDLKCFNFVRIFPVWICTTGFFLQAALTHRQMFLPIGQRGITSIKQTSFFYNFGYVGLAGYGLYLLGASYLWWQVTKMTGTKFYKHCLLGERQWSYERERQNNTYGNYYFKDVPLSCEENFPDLARGEIAKKQRPKPEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UM14</accession>
    <name>A0A8S1UM14_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0440071</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UM14"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8166288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="153" mass="18452" checksum="34EE886D36E702E9" modified="2022-10-12" version="1">MLPLFLPVLVMLAIIIPISFFQFLQQFSLVKYQKVKIHQNYQFWEVNHKRINDHLFDIQLGYIIQKETLISLILGVYLKLSLKYSRVFEGENIQIWINLEEQQLTALNRIIEFLFFKNRVDQMAEIFHNQIQRFQKATPNLQSEKYIKDDSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UNK7</accession>
    <name>A0A8S1UNK7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0440249</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UNK7"/>
    <dbReference type="OrthoDB" id="286213at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035871759" evidence="2">
        <location>
            <begin position="17"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8166640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="180" mass="20412" checksum="7BF54A63FD96B4A8" modified="2022-10-12" version="1">MYKSVLICALLLTTYAGHVRKSHGAVHQKRTFNSAFMEFVNLGDSDYHLNPKEAQHWAQITSEEVKHESKKHKSLVETHAEYIPGVVGQVTDLSNNAGVYSYTVTDSNGNIIEQDSGDHLAKQFNTAYLQMTQEIERGPAMIKLQTDLDNIMREDQMEQENKEEQQRQALEGTALSEQDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UQ14</accession>
    <name>A0A8S1UQ14_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0460056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8166765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UQ14"/>
    <dbReference type="OrthoDB" id="299752at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="97"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="213"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="287"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="369"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8166765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="431" mass="50773" checksum="A99947A835505E9C" modified="2022-10-12" version="1">MIRTNSTQKTKQVMLSNGQVQSLSPFNSTIRMSNHHQDAPSTPQRQIVSQNALRLIVNNHQAEVRQSNAFTPNGKQQQAGKCFQENRSVIVQDNTRMQQLINDNMYLSQALEQTKNELKQYQKSTHESQQLDFIKVKIESMEKVIDDQALEIEQWKQKYQKICTQDPQDSMLQMESQIMLVIQENERLNILVKNIQDSTDKKDQLITQQDQEIKKLKEKVKISDKTIQSQKEEIKQWHENFAEFEKNSKQQVSHESELLKSKMKIQELEFQLHKKKVSDNNNGQKLSQRKIDENPELELKLRQLLEENNKLTQLIETLQSENTKLKSTKQSRSNSCFQSNNQLSQGILEQIKQELFNQQLFSTEEFKKLKVIYQQNETLRNQLDNANEQISILKKTLQYYEDEQNVPSDQLFEEFLQSQSHCLNMIQQLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UQ87</accession>
    <name>A0A8S1UQ87_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0460153</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8166951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UQ87"/>
    <dbReference type="OrthoDB" id="1367865at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="261"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="305"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="349"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8166951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="505" mass="60419" checksum="12DEE7BE09A4A112" modified="2022-10-12" version="1">MLKLHQQLIHCPQHKEKIVSFNIEKHCPLNQRVLCKKCPTDGQKLDIEEVETLPQRHQLNEKHLQITSQFIQHINHQYNTLKQQIIEELDHFKKELDNFVHPIINQKEKFDYYLVPESTLTLKDFQELGQLLAENIVIEEDHFVLDFNKIVTQNHRQYIQSKFTDLELLLQFFRNINCNKQIEHQIQSRIQQQYEHNHQHTKLSKHSCQILQTNQNILSLGINYEESILILGLQDKAETIQDNLVVYSKNEQGNWELDQILQHHDSSVHHIYYSKKEDWFISVNMDSNIIFWRKQQNNWIQNKLKQEHLNFIWNVITSEAENIIVTCSEDSTVKIWFKHEDSIKCIQTLNRHIGPVYAIVLNNDNTVLCSGGEDKQLILWKFIEQQWEEFQVINVHSKKIMAIQFYGLKNIIVQFENYIQIIDRKSGQCIEQVNHNEYRISNLIYINDQLINYDFNGNLLIWMKKEDNRWYIVLKQIYAHKIKCVYGRGNELYIGEGSKVHRMQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UUK7</accession>
    <name>A0A8S1UUK7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Actin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0480089</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000048">
        <property type="protein sequence ID" value="CAD8167903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UUK7"/>
    <dbReference type="OrthoDB" id="286570at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000007">
        <property type="entry name" value="Actin like 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000148">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004001">
        <property type="entry name" value="Actin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00432">
        <property type="entry name" value="ACTINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8167903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="377" mass="42749" checksum="B596429F286CEAC5" modified="2022-10-12" version="1">MDYKKPAVVIDNGQAYCKAGIAGDDTPKCCFPAIVGRSKDQGNLKGMDKEIAYVGDEAWAKQDVLELNYPIDKGIVNNQDDMEKVWHHAFFNELRVIPEDHPVLLTEAPMNPKENREKLIQILFEKFNVPSSYIAIQAVLSLYASGRLTGIVLDSGEGVTHTVPIAEGYFLQDSVLRIDCSGRDCTKYLIKILEEQGVQFKSPFEMETVKDIKEKFCYVALDYEEEMKKYKESTANNRPCKLPDGNVIVIQNQRFRCPELLFKPSFIGLEISGIHELTFQSIMKCDYYVREDLFRNIIMSGGTTMLTGIPERLNKELTLLAPKQMKVKVVAPYERKLSVWIGGSILSSLSGFQDKWIKKSEYDESGSTIVHRKCICI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UVP6</accession>
    <name>A0A8S1UVP6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Activator of Hsp90 ATPase AHSA1-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0440207</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UVP6"/>
    <dbReference type="OrthoDB" id="567237at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015310">
        <property type="entry name" value="AHSA1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13009">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 HSP90 CO-CHAPERONE AHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13009:SF22">
        <property type="entry name" value="LD43819P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09229">
        <property type="entry name" value="Aha1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Activator of Hsp90 ATPase AHSA1-like N-terminal" evidence="1">
        <location>
            <begin position="62"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8166556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="159" mass="18460" checksum="5ADF16E95C0616AF" modified="2022-10-12" version="1">MDSVKKEHSYTYWVKNDPNHPKIDCQPKKVEDPTQVQQVQTIGSQWNVSGTWEEKKVPMNEIKKSLENIVGMKIGQTKISAVESVDGEAHLYLSRGKIRMGYHLKITYALEDDGQIKYTDFTDDGDRDYVLEDVTDETVKHQIEDLHLRTKEFVEVFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UWC5</accession>
    <name>A0A8S1UWC5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0490269</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UWC5"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000051">
        <property type="entry name" value="Protein phosphatase 2C-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="169"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8168744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="455" mass="52854" checksum="1CD895D77A04F674" modified="2022-10-12" version="1">MNQRIFDNIKSTSKRLKSPKIFKTEGNYQQQNRDNKIDKQNKAKSFINNRTGLGQRDYSEKLSQNTKNRLIVNNYSQQNRQQLNHTNNKYNSFLDSIFKLNKSKELNRVPIRNRKQMWHNYVNPDSSVPERRVQSQNNILKIEETYNKNRSTASLYSLEFQETFQFNLKYTHKTRQGQIANNPTKVNQDIFYCQTNFFENFHLFFVCDGHGQNGQFVSNFIQTNLPNTIRRDKVQIQSHQIKQILQKSIQNTSINVNQQSFDTNFSGSTLNGILLQENGKIYSFNVGDSRTVIGKLNGYGQKFKPYQLSIDHKLTIKKEQYRVIQSGGKVDTFYDQNGNPIGPMRVWVNGTQYPGLAMSRSLGDQVAQSIGVSSVPDIIEYQLGLNDKFIIIASDGIWEFLDNQIVVDIIGKYYQQNNIEGASEELMRVAYRMWTIDDDSVVDDITFIIIFIQNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UY33</accession>
    <name>A0A8S1UY33_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0500172</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8169123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UY33"/>
    <dbReference type="OrthoDB" id="292444at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="84"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8169123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="626" mass="73890" checksum="DE3EC362AAF9EA47" modified="2022-10-12" version="1">MSKKRLDRLDAGQCSTDRSHHKNNNSTDLNFTLHQRLNSNVDEQLQSKITLFSKFKQKELPQVNDSLQGMAQTLSKVSFNEIQHHQLQRDYEKIKTELAQFRAQQIKLYSTITKLQKDNQTLIEKLKFYDEEKLNLQRKIQIEQKQNEELTKKLKRSEQRIQIILESNIIFQKDQIKTTLLDTLHENEHYKMEAIQKQKDLQKLQINNNNLNHKLNRLTNRIVAAQRHQTESEFIDQDKMVITFTEIPTNFIFRHIFQNIECKQFISTSLEQTPEQFGSHFNLIPQDQKKIHLIWLFNHMVNYREFSQQHNNFIIKLTDLIVIKTYSDLYAFIQNMSQFLKVQRLTLWLRDYWTGFFETIQEGQSQKIICSKGAFKDCLEQREAVNKLTAQRHLLYQDSKGDDIYQENTYLYPLITDTVLPAGLLEVFQPTQNNSYSDIQYFTSLLGTFVKIVVQKIENDIVIQNVAKQKDILISQFLCLMQSNDKLQFTNSVKEMQSKLLQISTCDIIFIENNQMFKYNRQGLQSISCIAGVCGQIAQDKKPAFFSNLTKQVHYNQLIDMYTLAPVYIYPILKDNECKAVIELTLTNKQIDRHRFRDPLTSLNQQSNQARMFCECVQTAYLCKFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V178</accession>
    <name>A0A8S1V178_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PiggyBac transposable element-derived protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0530182</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V178"/>
    <dbReference type="OrthoDB" id="302124at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029526">
        <property type="entry name" value="PGBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13843">
        <property type="entry name" value="DDE_Tnp_1_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PiggyBac transposable element-derived protein" evidence="2">
        <location>
            <begin position="651"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8170675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1082" mass="124996" checksum="476846104E714A8E" modified="2022-10-12" version="1">MFNEELGVIDYEVESDDSIQLIENPQEIVISTPIQEQKQIPQQIVQQIPEQPNIENTENIERQQLEEEQMLRRPYALRWKKKSIKKLEKITSKMTDQDLDEIKDILTKKVVITDSKINNNNNYNNNNNNNNDVIISGSILKNPQTTLNQVQKQKQFQVLVEHFANKPDFLEAIIAQDLEQENANQDGGILKKPMIVPPKAGLKKQPGRKKTNVTFEDQSQPPNGGIQFIKTSVLPQKQPPPPPQPQQQIQNILSKFQEIREKSVAPTQSQQTTAIQEIQDVPLGTGAWYPNKQDRKSQPFIPPHAGSNPKILPTINSVQMISPSQAAQQSLNHKFLGMNQNQSRLSNSPPRVRDMITQQYTSSYQVDRPPIMPSRQQPVFQQQILEKNINYELNQVKKNKEQLSSHQQSQLRESQIKLQAKLQESVKRQSVAQPTIEENDSDFMDDIMDSQAFIDFKRAGNLICGSQQSQIQQQYWQEINFNTDIPKDYYMNEKFRVDVGQEIDICDAYELFKLYFDQSIFKYICQISNKRQCIKIDEDILESFISALIYIFYIQLLGMREIKRVRFDHILDYVTFGHICKEIRIDELEDYSFIFDKISKNFKSNYQPEEFLTLDSPIFYQNHSVEGLISLSDGMKGYVLDFIYGINDQKILQSLQKYQGKHHKVYLGPDVSSLNLISQLKKKQFGSLAKLVDKQTQLSQEQIQEVKQNAQKGKSTQLLSSDNQMIMLIYAERQQHLQQVEGFVSSFADFTRLKPQDTKIKSDVNKPIILYLYDKIKTQYDKRGKTYQYSQIPHQDANQHLEILVQLVYSSIYNANILNKIKNQQSSLAPDKAKQMYLEFVKQLLHSLYVRSFKRRGINQFPQNHTLESGDTGTFSCIECGESSQTICRECSNHFQMLIPVCRSKNEQCLKSHIEMLANQPKITNPNRKLTNKQAIVKFEFYRSQIEASKDPNAQSALPVIEDTLQQLDSLDPDEELSIGIQTLLVSLSDLIQKMFQQPNIFVPPKNQQSRQEVYSLNQIDPPIKSAQQLSKSISQIYNNKQEVVQSSQVIPQIIKTKIGEQQKNIDCSIQKLQARVAKINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V1C3</accession>
    <name>A0A8S1V1C3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0540157</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8171188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="293881at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="4693"/>
            <end position="4716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="4768"/>
            <end position="4828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="4694"/>
            <end position="4716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="4790"/>
            <end position="4828"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8171188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="4855" mass="565167" checksum="2919FD9D654CFBB4" modified="2022-10-12" version="1">[encoded data, 4855 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V1P9</accession>
    <name>A0A8S1V1P9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0540210</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8171270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V1P9"/>
    <dbReference type="OrthoDB" id="10603877at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8171270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="212" mass="25128" checksum="E76FA6874F606A00" modified="2022-10-12" version="1">MIILSNKTNKTQKILYYYYYQIIISHSIQENNQLVATFTFFFLHNNFQIQSVLGISYFVKTIARLKIKKIIQINPRKTLFSQEISIKPISKYKNKLKLNAVKIPSICKNIILSDLEHYLYFYIKQHQLILLKDINKHYKIYQFQNSINTVWKNIVLQISKIINFISSFSSFAFTLLQIAAPNKLNDFNKKLIHMKNIKEAKKVVKILLKFSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V2X7</accession>
    <name>A0A8S1V2X7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0540020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8170917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V2X7"/>
    <dbReference type="OrthoDB" id="304339at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="24"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="245"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8170917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="479" mass="56707" checksum="6DA42655ED5C8015" modified="2022-10-12" version="1">MQNIIQSGSDEETEFIFDSNTVQLNDSTTKISKANNEIIQLNDQNKELATQLNKQNELLDLKHKQNQQLQIEVIKLKELVDDRELKLLQSMKEKQQLQNDLINLHDILDQTTQRNDQLNSEVFAIRLTMEQLQQTTTMCKEDTNQALQRELRLKENLDQQTKVIKQNEETIRKLSNELNQKQTQFKIDIQNRDNIIQDLKRQHDLQLQQNTEIISELIEFKNQNLQNSVMSKDYKPELTKMTKDIEEYKNIIKGQTHQISILNEKILVLQNQLQKSEQSIANHLIEIQQTKLKLKEKDQELQIKLNENMLIIDRLNTQIKQLTNQLQLLKNQDQKINFVRSISQPSDEKFIKDLQLKTEQITMLQNENNQLVKLIETLKSQKSDNQGNEVLKVRQLESELKNLQSFIYDSPLVVLFNLLEDRLSKQQQKSFENKKKIQEFWSNQLKQWVEGFEELKKAFGGLKIQFKYDLKYPAQQLNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V6K4</accession>
    <name>A0A8S1V6K4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0530013</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V6K4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8170346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="16438" checksum="5EE8C74AEAC79A5F" modified="2022-10-12" version="1">MNNKIHQSKKNQNFQKNCHNISLDLLLQMQYQEFIIYYLQFSQELFQFRMMIYSLWEISFQIAIKTFKNQQPYQSTISFFVGIFDCYVQFLIYSAIKEARVFYVFFITKLLGCNSSFFDHIIFLQYNDFNKVLNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VAY7</accession>
    <name>A0A8S1VAY7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0580259</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000058">
        <property type="protein sequence ID" value="CAD8173189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VAY7"/>
    <dbReference type="OrthoDB" id="10257263at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12736">
        <property type="entry name" value="LANC-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12736:SF7">
        <property type="entry name" value="LANC-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8173189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="422" mass="50794" checksum="B6ECBD115A8D1B40" modified="2022-10-12" version="1">MEQYIFCYHLFLINILIIIINEICKNKIQDILNSILKILFPIIIISKIFLRHLFLNIGILEQKYLLDVQLINAQKNKRRELIQMFLWSRGIFRESMNLFKLQLQNEAIMNKRIKRLRVFRILQFGAFLQLADEQYQAMQPQIKADDDISFFKSNSTIYFLGVDLFYYMKDKDNFKKCSVEILGLFGNIKYSNEQEPFKLELTSQIDNLCKIIYQFTGNGLLKYNFHNREYFGAAHGILGVIQMLLLSFMSNAKNIFIQKMNKNYLKCQNPFKQHQTIQLKFIIKTVIFWCQLKIKKMQNYISLLWDTRSYCSNFKSLQDIQQIRLLYTAIHMIEQLYKLEQLRKIMGYGIEFKETNLRRYAYQFLQYIKNPYIFNEIKNFPFNDRYIIGISDNPFSLMLRNIRDMCAMMDFINYKRMPGYEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VB53</accession>
    <name>A0A8S1VB53_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0560099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000056">
        <property type="protein sequence ID" value="CAD8172126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="77931at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2204"/>
            <end position="2222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2260"/>
            <end position="2279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2311"/>
            <end position="2336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2348"/>
            <end position="2365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2399"/>
            <end position="2425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2463"/>
            <end position="2489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2538"/>
            <end position="2560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8172126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2662" mass="311215" checksum="F5DADE361B562E12" modified="2022-10-12" version="1">MDLKIQLLFFLLFFLILCSFLNNLSSAFTILQFKLKNHKQALSNLYVDKKKILIRKSQLTLNLIYYDCIDDSLKKISKTIAFIDNNNVQHSFTIDLDSFEYENIWYYFQILQWPFQERFELIFIYQQKTIRENLKITQPFKNQSLMLIFGGGMIVKQSMISTITQGTKFSYYPGKMIQVEYEFGEISVDEDQEELARMTFEPYNLCNCIINKKLRIEDKSMKYLDNLVFTSENINCNSFELSGWLKITDIINQSQDEFIYPFLILSSNFKNSLSKRTLSPLTLSYKISGIINQIIITTYSYTFLSVISDDQQLIEKEFDIQNSITLWQQLKIKLVDDQLIINIRFYENYDFYEYSTYEKVKQFKCTQYKLKYGNINQSKTNYLNILFRNILFNNCETDDTLQSCHHSCLECDGPPQNNCLSCSDLRIYLPEFKACICPFNTIDKENCVTYKDSNLELSNLKKVMSKCKYGYFEYENDCIRCPSIINDQVATCQECLQNLQGWNKEPYCQNDLYLNQNGDVKTNQFNSDYIYFLFDGIDLIECLKCNSSYSFIVDAYDVQSEITLKQQTFSFIKELDGCFKSLKNIHSYYCIQCYQYYQLKKAKFQLNYILINEYSIEQFACLSSEYLNSQRQYKLCPILNCKYCFEYQSNDLSKCTLYNNFDDFDIDQEIKIGCALCEDNFIFDFDIGVCLFNTPKIQNCLKSYIYDGQEVCTISQIHDFLLAPEIINCQDLCPNCLQCIQSPEFTVKCIVCQIGYITSISTGGCYLADEYKQYGAKIIIEGVYSKMDGWVQRIQSFMMKFLPNQYFYFQSKFDQVLSQIVVECIEGYRLSPYFGCFKNCDPLCLDCKYNTIQGYYCNTCPLNQYYQPIRNEIDGKCFQCTQLCQACQGRTNNEIICLKTVKHPQIYLDPYDQIAKYCFDENCSSIFQFQKIIEECYFFHIMWEEELYIEYLNKLGIDSLIVRFTFTSTWYICQYANFFIDSGITIFLNFVIRNSTFLHSYVFQFINNQVILSVKNLIIEDCLFTNSIFFLFDSNIQQLSKIEFHNIVIKHSKFINSSFFNCQNYVSLSLVNLQFQNNSALYSNIFFSSNQKFSLQYFQVKQNQFKDSQFIFVIEKNENLQSFLVFDQFEANNNQLYNSKFLRIQTNIESSQVDLVITNLVFDEIVNVNNSDTNQYLFKIRCFKLQIQDLIFRNLKNLLIFNIFEGKDIMISNILYENPFQIYQLPFLQNCQVKNNDKNQLLKIDNFHNLIINSIQIINQFTIDESFVYFSQAKEFSNSSKLEISDLNFIGNLQLFKKSQKSFSLLTINSEQSIKVVVNRITFLENSFHSQAESIWQSYASLIFLNIKSSQVQITNLLSERNAFTNSSNSFIFINSKTIFMQNLQISNHNILSQKLWEKYYNVKLDQIFNQQQINLFALQALNIKNIGGVAQITATMFTCLNCQFYNILASKSAIFEIKTEDFGIINLTLIKANNIISDLKQVYNSSGCISIYTINSLLDITIQQSEFKNVFNRMSSSILSIKPSSIKNIINLIDIQIYDCLSLMNQILLVEFSNKIIDQNQLKIQNIMITQNLDSSILLFSQMRNLSLTELLDISSSQNSLFYIQGCSMQMINVSVVGVYLSPILSIVNAPIVKLKNLHMEQLQRFYSFNFININQMIDIESKVLINKVSILNSFTYQAGLQKIIIEEFNFILDGCVVITNQSNVTFGNQNIYFLNDILQEMEKHKQTPLSLIYIKSLQNKHSFYFNQLQIVENNCINYINGLIYFDLLTFNYIKIQELNCYYNVIQQYGCLKIVDENLQSQPIQVISSNFIQNTGSQGVAISSNSPILIQNCKIISNTASEKGGGIYFKLRSDQFNFKNSIIIGNKAKEGGGIYFSGDSNLNQHNFIQTYLLYNYAYDYANNLVESPTHLALFINSLEIQGKQQKFHNIQTNILTIFPYMTIEQEIKIKTAYLMIPSGSTIQGYSLVIPKQSSSIRQIQQIVLLYKNNLNELQKNLGNSSCIITENIVKKNEQIDVNQQHVQLNFNAEKNHFDLSSISFTLDPYIQDYKYLEIDINCSILEYKNSLKYIIQAKSYKFQLGEFYVANGCQLCESNKGYYSVTYDSNKCSIFDKEKFEEISSNQIKLLEGFWRPNYLSDQSTQCFKITRFCLGGWNVGDALCSEGHVGALCEECDIYNVQGNGKFIKNQQNSECFLCFSTQDSIIPFILNTLWAILSIILTLKSIGKSNNLFLFYKIQGSFNQILFKLNQDFEGILIKMLLNYLWIFSVIFSLNINFSFQFNFVDQASNQSYSMANNLDCYLSEIANVKLIYSKIILILILILLQFVIIIFGLIIFSIFNNQKFKLDNISNTLLCLYIFNYAGFIKKLCSVVSYREISNILYISGDLTLEFNTDTHYKWIMNFVLPGLIIFGFLIPFLLFLLMYFKRNDLNNHIFRPHICYLYNEYEKQSYYWEQIKLSKRAIMILILTYFETNIHLKASLIGLSLIIYQLIAINKKPYIMTKLNKFDLSTGQICSISIFLSTIKYESEKLNNKAISLALQICLMILFVLITFPFIHNILQIYYKKYKLLILKYLFLLFQYFRLKQASKKIEFILNEKQQKEQRIKDNDLKLKQFLLIISKAQLKYRPAFESTFNSRKKYQQKYISSLMQGQSILLTGQRLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VC80</accession>
    <name>A0A8S1VC80_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0570117</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VC80"/>
    <dbReference type="OrthoDB" id="296179at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05580">
        <property type="entry name" value="STKc_PKA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000005">
        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF37">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT PRKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="11"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="10">
        <location>
            <begin position="266"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="294"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="309"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8172486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="318" mass="37158" checksum="AF8B0CF60E9BC601" modified="2022-10-12" version="1">MQNKQVKLQDFEILQTLGTGSFGRVRLAKQKSNQKHVALKMLKKCEILRLKQVDHISSEFKILHQIQHPFIIELLGYTQDERYLYFVLEYIQGGELFTYLRNAGTIENDEAQFYSAQIVLMFEYLHSKTIVYRDLKPENLLIQQNGYLKLTDFGFAKVVEDHTYTLCGTPEYLAPEILLNKGHGRPVDWWCLGIIIYEMLAGIDPFNDEDPMAIYQKILKGKVKFPKNFDNDAKELIKNLLVSDVSKRLGNLKNGANDIKQHKWYQTLNWDNLIKKQIRPKYMPSVRSDNDTSNFAVYPDSTELPDPIKQNDDPFRDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VF76</accession>
    <name>A0A8S1VF76_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0650057</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000065">
        <property type="protein sequence ID" value="CAD8176388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VF76"/>
    <dbReference type="OrthoDB" id="296828at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14003">
        <property type="entry name" value="STKc_AMPK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:002041">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF82">
        <property type="entry name" value="KP78A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="7"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="391"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="512"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="391"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="422"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="433"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="466"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="521"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8176388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="745" mass="86620" checksum="1B70CBBFD49DF540" modified="2022-10-12" version="1">MTELGNYLIGKTIGEGTFGKVCHAKHQVLGHDVAVKILEKKRINDELDIERVKREITILQMLHHPNVVQLYEMIETDTHIYLFMEYADGGELFDYIDLKKRINEVEACKFLHEIISAIQYIHQLRIVHRDLKPENLLLTAQKNILVVDFGLSNTYEDTLKTACGSPCYAAPEMIQGKPYYGLQTDIWSCGVILYAMLCGYLPFEDNNTQVLYKKILTADFHIPRYVSLDGKDLIKNILAVDPTKRFNIEQIKQHKWWQLWKSDNVQMTPFKASFNKISCTLLPNSIYKSCVKKNIERNISEDVKTCCDTAQQSIDIQFTDKEDFINKSIIKEEIKQNPQIQKVEEDLDQQFTNVEDHFEFQQSMRSLTRQKSSQSIQSITHLHTQIDSPLKQQNQAVKTKQSIPTKQQNSQINQVIPKKPVLTQQQQQTLQQKKSITKTVPSKSLHEPPNFESKLAKQPNTPSAKQQNNNSTISQNPSNNSQQVSHHLKTEIPKKNSIPPVKLSIIQEQLKENKEKSHSLKTQPTQTKQQQIQQQAQQQQQQQKQQINLNKPQNLYKTPIKQNTPQINSTKRSLHQKQASLQMERQSFHTQQSNVEENSILQFNDNPKPSIENTKLMVEKFTSRLNNVIIPSQLKQTQKQGIQDIKGIENYYGPYNISFITTKHPQLFLKGIQKYLEEKYQLIQIQNFQNGFMFNINGLKIDFKLFRIEHLEIFYCQINCENSKNIVNNTDFNTIIYDLQTNFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VG73</accession>
    <name>A0A8S1VG73_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CoA transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0640102</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8175967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VG73"/>
    <dbReference type="OrthoDB" id="421515at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008410">
        <property type="term" value="F:CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050483">
        <property type="entry name" value="CoA-transferase_III_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48207">
        <property type="entry name" value="SUCCINATE--HYDROXYMETHYLGLUTARATE COA-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48207:SF3">
        <property type="entry name" value="SUCCINATE--HYDROXYMETHYLGLUTARATE COA-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8175967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="381" mass="43153" checksum="8B8D1CBC7C2D69E4" modified="2022-10-12" version="1">MYKQLKVLDLSRILVGPYATMLLSDLGAQVIKVESFEGDETRKWGPPFFNNQSTYFLSINRSKLSICVDLKKGNQFIKQLVEQSDVLVENFTSGVMDRLGLGYDELSKINPRLIYASVNGFGSNNNQPGFDYIMQAETGFMHITGEKDREPMKVGVAIIDVLTALNLCNGIQGALYQRIHTNRGCHIQTSLYESAIASLVNVSGMYLNGQKDMHRMGNQHPSISPYGTFKIKNKYIVIGVAQDNQFQSLCKILNLNHLINDEKYKENKGRVQNSEKLKRIIEEALENWDVENLINEMKKNKIPVGEVKSVGQCLDSQHSKSLNMVIELENGEKIIRNPLSFSNINLDYPKQAPYLNEHRDEICKQFGINNVDKLIQDGILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VGH3</accession>
    <name>A0A8S1VGH3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0640157</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8176077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10332084at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="265"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="295"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1315"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1429"/>
            <end position="1451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8176077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1670" mass="200384" checksum="7DFEA8EA59EBF008" modified="2022-10-12" version="1">MEHLLVNFNLGKLKRPSYSYSMLLISITFCQLISAIYSKYQLIIAYHYPNLQFSQISQIINLIKYIDVNSIIQENEIIIQILYWWLTTLSILTYSLYIICYFQVKYQKKKNLTDQSSAKMLTQLLLIDQWITCQPTQLVYLNIAYCSINQNCLNIGIRTDIQQVLASFFLFLRFITQIIMILFFTNHQKIKKDALSSGSRFILLYLEVFRVLLNIQLAFPIQSQQFHITILIYLILSTCLFSFLFKMNDFKLFIANRNVRQVFKIYIFSLFGISIGLVINSFSIHFNIIQSDNSISISLLSVISITWIFYQYYFNQQIEHLCLPTLTGDHLVQKIINLKSVAKQLNILHQFGEADSYYLGMLSQHFSNICIQDKKERCFCRMRILYDPKKHKDIKTSQFSMLRQKNLYLKYLIKTWYEIYLFHHPSDVDIRMNYALFLYYQMNNQILSYMQIKLTCTYKMNLIQQFDINKIQLQFQNEIIDTNSLSYHNESDFEYVIKIEQLIKSIELNIQNFLDLSIKFWKQVNQKVIYEEDLWNINKEIILTIQQSNDLWNQISKHKIINRDKLIIKNFLIRRPKWQFLYNWYYLYVLNKKLKQSMLEYCDSQNMVQQPIDEDSGSDNQNQIDQFSSLKTFNYTSAIFHTSQSGQIVNFSESTYDIFGFQQYNNINQLLPNTFIRNHQFGIDQFIQSGKSKSLYKKRKILALNNEKYLMPCKKYLKWHISPLSQIEYICMIRPIYRHQSVNYILVNSDWEIDSVSEQIAEYFQPGLCLFLLCPKLLKYSFYSQFLNEDDLQLFKLKRAKNDDNNQDSIIDAKFTNRKITITPSNQKNYFMLQQQTQDIVSMVLEDDNKNVQDSPLWINDALHEDLQEEKDQLFKRFHKVVDEDHVKLTFRLPKKMKNLIEDYTEIKQEILLNGLDQMTIKSNKFKDQRIIKRNSNGKLSFDKKVLFSKLFQYQEYYYENLYDHFKSSFQRYCMTSRMLKTVIKVEASIRFTKNTIMDKYQIIKITNFNVIESQILNKKAKVVNLINKKNQIIRQSRQSIFQNPQQQQHLQQILEQNYQQQIELNYEKLIHSNIQNSDVESYRNTNRPFMSENQVISDFNAQQITSLILKTQNNIFIKEENNKPAKQDIQLNFFKILNRIFIFFLIIFNLIVLYQGPNINQQDMDELGFQSAKKQYDFINIMIESYDKLINSFYFKNNLLIQINNQSEFYQNLQNNFNNQLFEITKLYKQPSFVQKIYKSKNYNDNLNIIDLINQYTSNIGSLQQIDQNYDFEQSLDFFRTIFIPFLFNYQSRNIYELIDNLTQDYQNQDYICFTIIMTSTALFGFYLVINILNILKLITQSQKVAKQLSYIEKTQVEEFIKFYMNLKFQFSCIRSTNGLIQNNYFQSTNQTIIRQVEEDEKSSNLKRQKQKQYEWSKRWKKIKVLMILRYLLFMSMMSFLSYFYFIYILRFNNQMSNILISNYFTKQTNQLISLTLSKEIFIYNFFNQTYINISNYKEIQLRYVENNAELQSQLYQTNINEIDEIFNGNLCTTINQIINFQYCNTYLNGALNYGLHSYNFLLSQMAQQLLNPDEGLYDNTTVEKIFEFDQINQFQRIGYEQAWTILGLRYSQYIAENSKLEIIIFFFNQMLSIILEVRCFYKRYFSNITIDRYKIIRAQLIQCKIINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VHI4</accession>
    <name>A0A8S1VHI4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0600279</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000060">
        <property type="protein sequence ID" value="CAD8174206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VHI4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8174206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="102" mass="11763" checksum="1106EFA81A5E6D85" modified="2022-10-12" version="1">MTQNLKFFFADNSKSQDPHSSSFSIQKMQNSSQHGGNMGTQINNSENIVSAAIYLQQIDEKYSQMYMNKQIDKAKKDKSHNQKPSHNDHTQVYNNMDVTNKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VHT1</accession>
    <name>A0A8S1VHT1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0640076</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8175915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VHT1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8175915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="75" mass="9345" checksum="5DF065DB93743521" modified="2022-10-12" version="1">MPQNFQFQPEKSYLKLIQSYKIQSIKRSITYFIYIYRISNQYKQRYTCSIELIERTNDQKSFNWNQESNQYLQLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VJV0</accession>
    <name>A0A8S1VJV0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0630235</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VJV0"/>
    <dbReference type="OrthoDB" id="320626at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8175732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="183" mass="21517" checksum="3F028749E9FD38C2" modified="2022-10-12" version="1">MMIAIENFNYPGGGLYDESGNECKIGRWIEINEEFDNISQVTYHSDYKFGKKLGRWDIWYQKNYHDLKNGKIGGESYDEVGNEWKIGKWTELSHNFRDTSQVTYLGEYKNGKKIGQWEIQFEAEKIGGGLYDEEGDMIKIGKWIELHDPFTDTFQTIYEGEFKHGQKVGTWIQKNRDENPLDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VK15</accession>
    <name>A0A8S1VK15_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0640158</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8176079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VK15"/>
    <dbReference type="OrthoDB" id="298687at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8176079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="257" mass="30253" checksum="39D93A998EE75083" modified="2022-10-12" version="1">MKTLNDIQKLNQQQKTIFTFTNQITNNTNSAWLPRDIQSPFSNAVTQLSPQLSDHNIIHNTRGNLRSWSQSIEKQIISRDLVNKARIQNAQQSARQEKHFLGSVNGIQKINSPKSQANQTMSTHSSSRIDPKVKRKLSQMSKGNFLHFLLIQKVGHKDEKQFYDYLKKTYNFEIPQPQQEQSTKINKKDIKYLFTIDDDDETIKKTTQQIFKMNKFRHQKEVPLDKYVQDTYESQLRRFPKVRQKLEKSKPYNIALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VKE9</accession>
    <name>A0A8S1VKE9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Smr domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0680093</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000068">
        <property type="protein sequence ID" value="CAD8177804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VKE9"/>
    <dbReference type="OrthoDB" id="3231855at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002625">
        <property type="entry name" value="Smr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053020">
        <property type="entry name" value="Smr_domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47417">
        <property type="entry name" value="SMR DOMAIN-CONTAINING PROTEIN YPL199C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47417:SF1">
        <property type="entry name" value="SMR DOMAIN-CONTAINING PROTEIN YPL199C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50828">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="626"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="691"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="719"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="domain" description="Smr" evidence="4">
        <location>
            <begin position="882"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="221"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="795"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="229"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="246"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8177804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="961" mass="114630" checksum="16A938794700B536" modified="2022-10-12" version="1">MSQKIKILSTSTQRTSNDEKNDFCSVFLGFDSKDQKNAGILNQEDAENDFHQFLDNPNLSKSQKDDKNYRDLVLNQLQGQDGSTQFKQNPLENQSNQNSGAIPLEDNYNQYSNNLNEYITHYNCQKSNQISQNFNNNPDFYYEDYGGVQNIQDFKDPIYYQDNENKEFNVYERYYKDEKCPYLQPIQVKQLDQDGMIYNQQYIYWNQDEKEPYIQQQNDEIINQDNGQIKKKTNKRGNRKNKNKKRESNNEQEDKQQIYHEQHLQQKVIQDNNTESTNQFDNGNMEELDDAVSDEFKIKITNFLEQNDNTKISLTKTIYGNEKQSIKIIEIIDYENQIQKLCKQFSNLKYSEIALSLQLIINFENCQQFLQTLKPKEEKLILREINQLIVDEQKAIESKNKDELTKSQYKIEEFKKVNQIQNIEGQSLYQTAIKIIEQKNFKFDIKKCDQQTKQQKKADFELNRNQVIAINNQKAKVIKQYQTCNINENQCNQQLQQIRLEKKKLEKIGRNLFLDLMVIDDNGFCRIDFHKFFSAEIENILDGVFEKIESYKLCNQQKQIKLQIIVGKGLHSKNQKPIIGPITKQYIQQYLQQQVEIIEGKIDFDQLQSQSKVIFIIIQQQIMIKLFLRLLSHFLFYWTIYIIYINIYSIFEQKLSLLEFLTPTIDELIYLSFSTLLTIKWYLIQQLFNLLFYLISTLLVIVWHFLTYIPSIISQLDTWFLIISTVCIIIILINNCKFGDYEDKYQQVQYQNYQVNHKFPENNFENYYFTQNNDLYKTRRNSYSTFQTKSYSQNKRQQSQNKQIEKEERKKLIQKLNENRVQMKLLQQNKGEIFQLKKEKEIKRVVIKDALQICIQKIETLQEEGKELFLKLINIEDGICRVDFHNFYKVEIKALLDGLLEQIRLYKYRLGYKYVKLKIIVGKGNHSKNGIPIIGPQTQEYLKEYLQEEVEIQNGFIEVQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VTS4</accession>
    <name>A0A8S1VTS4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0720081</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000072">
        <property type="protein sequence ID" value="CAD8179515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VTS4"/>
    <dbReference type="OrthoDB" id="1926212at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050498">
        <property type="entry name" value="Ycf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE SPINDLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="256"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="24"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8179515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1120" mass="130849" checksum="D4F0FEB6629CE788" modified="2022-10-12" version="1">MKNVRESNSFSRFKTRQKSRDNDQNSQILKLQSQQNVKKLSSKAQQGSFHLLPESNYRPKTKSMVQQVIQKPSKKVLNDTHNYLSSKYQAKKLIKQYSFVKNRSKKMHSVSYYHYDSIQNENDECFTHFNNMSNVSFKDYMDAEARANKMPSDKSQEIIEGKKLLSLGQTEEAQKLFEDILQVSNNPEARYLNGICYLSKSEFSEAIADLNLLIQQQPLYKRNAYILLAIAYKKSNCPNDALNTLTLAIKKFNKYFDAYIYRGKLLLKMKQYDRALKDFTSAVDIQPKKAIAYIGQADCYRYMNQPKQSVQACTQAIECEDSSFRQALIKRTLLYIDLKEYDLALLDIEAVLEEDHCDSEALYIKGFICTKRNQIQEAFLAYEQSIKHNNSKKAVSKSLYEIAKIKIEQRDFYEAFYQLSRADYLDVDEKILEKFKIFTDGVTFLMKRKFDEGVEALTTLINKHQVTDFLKPLIFQYRAYGYFCQSQYQKSLNDLNQLTSLEKPSIYNKLIVEGILAAVANQYEQSQGFFLKAQKLMPNKMEPYFYKATTLVKFYSYLIPKDDIEKKNKFLNDALKYMDMAVKINEQSNLLFYRGIVLFAQGRLDESLVDLDKAIEKSEDHVAKHFYVRGLIQACRNAFELAHNDLTIAINLDEKLVDAYLNRAKVFLSLGDRKTAYYDAQKYKECKQTDPQTDIIIGNLFFQIGAYEDAIQSYSNSISSEKSLQVLYYRAKTYILIKELNSSMLDLQKIVEQSNEIHAIVDLNILQQLKNTSTANSDQNLFQEALQCANQILKKGAEGKIFKKSDILFYKGLFLFYLKQYENAQQALRESYKIKENQQRKELEMINDFDQYILDQLNQTQKKIEFRSKPLEEFEFSDRTYNLFEYYFNRSAIHLLLGQTDSAIDYLEQLHENIAQQEIQEHLSMFIQLLKDDQNPKSEINPELSKLTEFIIFPQHNRLCSIYPMVKLPLKKYKQSLLLRLSFCLPTIEIPEMNIKFDDKLLETISPTVVENKPEAPWIKRTAEGVIFTDNVQIVEDLDLQSTTRPSKKQTEIEDPEFQQFEEQLNNAIEKYDYSEIDQQDEIVEQQTQAIDLQQLKQNLMLDSKIADKLNSILQKKAQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VTY2</accession>
    <name>A0A8S1VTY2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Heat shock protein 70</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0720111</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000072">
        <property type="protein sequence ID" value="CAD8179575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VTY2"/>
    <dbReference type="OrthoDB" id="2401965at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11733">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000036">
        <property type="entry name" value="Chaperone protein DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000014">
        <property type="entry name" value="Chaperone protein DnaK HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000004">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000003">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00332">
        <property type="entry name" value="DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012725">
        <property type="entry name" value="Chaperone_DnaK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02350">
        <property type="entry name" value="prok_dnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="628"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="633"/>
            <end position="663"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8179575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="663" mass="71920" checksum="CC25352B5571BFFC" modified="2022-10-12" version="1">MLSKLFRPSLKVNQHTVNAFSSKVMKGEYVIGIDLGTTNSCVSIMEAGTPKVIENAEGMRTTPSVVAFTADGQRIVGAPAKRQAVTNPENTVYATKRLIGRRYDDPNVQKDIKHLSYSVIRAQNGDAWVSLKSGQTYSPSQIGAFVLMKMKETADAYIGKPQSKAVVTVPAYFNDSQRQATKDAGKIAGLDVLRIINEPTAAALAFGLEKKDNKIIAVYDLGGGTFDISILEINAGVFEVKATNGDTSCGGEDVDSILSNWISSEFKAQSGVDISKDKMAVQRVREAAEKAKIELSSTTQTDINLPYLTADASGPKHCNLKLTRAKLESLTEDFLKKTIKPTENCIKDSGLDKSKIDEVILVGGMSRMPKVQKLVQDLFNKPPNKSVNPDEAVSIGAAIQGGVLKGDVKELLLLDVTPLSLGIETLGGVFTKMIPRNTTIPTKKSQTYSTASDNQTVVSIRVFQGEREMAADNKLLGQFDLSGIPPAPRGVPQIDVTFDIDANGIVHVSAKDKATGKDHSITIQSSGGLSEAEIQDMINKAEKYKDEDKKRRELVDLKNEADGAIFNTEKSLNEHKSKLQPNEVQEIESAIQNLRVLLTENLTANDVQRLKDAVEGVKNAAMKIGQAMYRNTGGASEQQQQQSSEQTGEQQNQEGGDNNNKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VV04</accession>
    <name>A0A8S1VV04_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0720138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000072">
        <property type="protein sequence ID" value="CAD8179629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VV04"/>
    <dbReference type="OrthoDB" id="293750at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="33"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8179629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="230" mass="27049" checksum="50EB9BF4F4CFFCA5" modified="2022-10-12" version="1">MFKLRTHTVKQWLLQRFRKSVNSLYITDPQEEKYKQELAELFAKFDQEGKGRIQKYELLTLLNKYGISINEQELTCYIKQANSKSGHFITTEQFKQCVLSENSKLFLKKLIHKSNERNPQNYYPTEITKVLNQVHYLSERGRLLSQIEDKLIPVEQKLQPLSKLMNLSREITITEQRPQSKLPLRLPRAKSTCTTNYNSISTAITLPSSSNSRFGDNRISFRRHPTQYCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VXI9</accession>
    <name>A0A8S1VXI9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GAT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0780237</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VXI9"/>
    <dbReference type="OrthoDB" id="2018246at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21383">
        <property type="entry name" value="GAT_GGA_Tom1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03561">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004152">
        <property type="entry name" value="GAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13856:SF137">
        <property type="entry name" value="GH05942P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13856">
        <property type="entry name" value="VHS DOMAIN CONTAINING PROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03127">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50909">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VHS" evidence="2">
        <location>
            <begin position="12"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="GAT" evidence="3">
        <location>
            <begin position="172"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="364"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="445"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="272"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="369"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8182494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="563" mass="64892" checksum="E7E7EA703BBA932C" modified="2022-10-12" version="1">MENPVERAVNEATADTLQMPDQKLMKQVADLVNQRADQSKFAVQAIGKRLMQLRNGKVQALTMELIEYLAFTCETPFYTQIATNDFLQRLNTLLNPQMNAQMQQRLLQVIAVLKTLMQSHQDLFPAFFQFLQKISLKHQLPNNYESKYAVLRKAQPTYSTGARVNSLGSGNSKNDKLRRDLEIVKSNITLTNEIIDNANPQEDASKNEILKDMTTTLRGVEEKLRNLITDMGNNDEGLMNFCLELNDDLLRTFTRYEVLKKHKKPDPFRPEQAQQQQQQQVPQQTLAQPQTIQQGPFQQQQNPFSIPPPNQQPIQQPPQPKPQQIDLLNLFEYDPVPAAQQKGPQPTQAIQPEKQQNTIDLFDVDMSNPQPQQPQLTSIITQQQQSQVSTQVPQISQLPPISQIPPMQQFGQQPIQQSQLYGQPPISQIPQQSNYSALNAFPPQQQQQYLPQQQQQYPPQQQQQYPPQQQYPPQQNLQQLYQQQQFGQQQIKYQAFQQQPTQPIPYQQTQPYQQQGFPQQQYPVISNISAQQKKPENEFDNILNLAVGQKPQAQQQQQKLDFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W492</accession>
    <name>A0A8S1W492_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0800051</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W492"/>
    <dbReference type="OrthoDB" id="286004at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="1">
        <location>
            <begin position="211"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8183045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="300" mass="33278" checksum="01E459916D402053" modified="2022-10-12" version="1">MFSRILKQNYMTKIALFSTASFIYWGSRKNQSEEIFQILNKQTQNSIVKFFLNGRIIGQGAVLKNGFVVTSSEVFENQQPQISAQINGQLQNYEIVKVEEGIAILKIQQQKSQFNLSKYEIGQNAYVLGTDEQGLKEFQQCPITDSNFTLDIRLVGEEALNQTFPFILMNGCNSSPGSPVLNKNGDLLGIISGKFRNKTQVVPSQYFQGLENGKSVVKPYLGLTLKTSDQGGAFIIKINSDSPAEKAGLKLGEIIKSIDGVSIQHGKDVTKLLGVTENVDSHVMVVLRNGKERTVYVNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WGP6</accession>
    <name>A0A8S1WGP6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="6">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0900053</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ubiquitin conjugation factor E4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="6"/>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8187807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WGP6"/>
    <dbReference type="OrthoDB" id="312224at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034450">
        <property type="term" value="F:ubiquitin-ubiquitin ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000055">
        <property type="entry name" value="Ubiquitin conjugation factor e4 a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019474">
        <property type="entry name" value="Ub_conjug_fac_E4_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045132">
        <property type="entry name" value="UBE4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13931:SF2">
        <property type="entry name" value="UBIQUITIN CONJUGATION FACTOR E4 B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13931">
        <property type="entry name" value="UBIQUITINATION FACTOR E4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10408">
        <property type="entry name" value="Ufd2P_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="11">
        <location>
            <begin position="1049"/>
            <end position="1123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8187807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1136" mass="133869" checksum="EE0BEB5E0B1FE52F" modified="2022-10-12" version="1">MDCQIFRSKKKIEEERKSQESLVENQMYIIINDENKSQQKVQGRNQQIKYSRLTINHLYSLSIISHSSKKLGYSLQEMNLKVLPKQTNIDQKIQNYRGVAEMNNNKQVFQNQQQIDVFRVNNNINQQNQQQQNLQQVILQNNTNQQSQQQKQNQEIQQSIPQQQIQQIQQNNQNINSDVQQENEITTPFNEEFIFKRCFMINVNELKIGKLYAQMVKQVKTFTLEIILEQIIPIMINNKQLLQSQTVTWLIQVLINLSECDIKSTYAIFEDGVISKEILIILIDAIIDKIVCAVAKPQQLQWQADREEINKYNGLENQIATQVYDMLFGQMIKTNYEISFENLEHPALNYLLTYLDTKCQPEDAFRFLDLITKRELLRCQKVDLLDCAYQLKASLLLDKLSFYPKLTDLLFQNSWAYGKFQIYKFGRDIQQYSIFGSIISLSTFPGDFPNVKMIFGNQEKGLLILQETYRGPIYEIINKMADIFLRIIRRGKEAQLQLFTYIIKLIEINLNLEKLSQIQTINRCCFQGMMFNLQQILLEIFNPFIYNTEQANARLSKINLDLLASVKTHPLFSKIYSNAGLMAPLKTVFIPQDKSPVLDPMTVLYLLIQRIGGLSQRIINNYAHSYVDYQIDKQFFGPHSRITQLSEIEKATFDTLFLHPKSIQNTIQFLSFQSKLALSLLDENYKPKYPYGLLYNQFMQDIPNYCFIYNSNGVALDYLDEVISIVEFTIITMKYQDLIEDIHLRCKGIELFYIFNEYMILYDSKNALMSFQIFQENKIIQNFLIQGLIQSYIDRDRIKMSSILSAIRFKKAICQLFKYALTNHRNIYENKFLEFVNMNSTTYQNFAFVYINDIKEMVDQCVQATKKIKLEEETLANQYSLLNTQEKALKELLRLIEVNRCIVDWESAIELFNNIILFTKIEPKAFLIEETRQQFAQNLNDVLQQLNGEGNSFMKAKFLVQSQINPQDISKNIIEIYVNLQDQPLFWDEIIMNNQQLQIQSLYLLINNMSNQYTIPNDLLYKFSQIINNLQQLRNEFDQIMNDLSKDPNLEDRYLDSLTRNLMTDPVMLPNSKQIVDRVTIKRYLIKKQEDPFDRSFLVAEMLIEQKELKLEIKQYMDSKKNQLRQLRGHTQNFYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-06-18" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WIU7</accession>
    <name>A0A8S1WIU7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0910219</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000091">
        <property type="protein sequence ID" value="CAD8188577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="307499at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050349">
        <property type="entry name" value="WD_LIS1/nudF_dynein_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44129">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2198"/>
            <end position="2229"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="42"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="732"/>
            <end position="770"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8188577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2747" mass="325458" checksum="D34094EFB13C0C8D" modified="2022-10-12" version="1">MDINPGHMNLNLRGGGGCCGFLLNTCIKKDTRRLNNNKNPIIDIIKEEANSLKQLISSQQNSPQMLQKAKESINKLYIENNNLQLNIIRNNSFYQFSAQNEGDIYEILLNCLISLDGFIKISKIKSHQLIYLCLDLFYFIYQIQFRDKNFFLNNQYCDKYSIAIENLKITAQQDYKYEISIIHIAFEKCRKDNKMTFCDSIKGKLFLSFEQFDIVKHFEENKDLLNYNYEQTDISTKDFQLYSFYYVIKWSILKDVQNYYKKTLDEPINILQYTFSQHCSNNYSFILQKYWIQIIFELIAQAKFQPIEDDENSKLVQSKFQKLSEILIEQYLKFYVKLEIKDTNRENLIIDPNYQTIVLIQNYSQTLRDIIEKITLIILKLKNKQNQDLLANLKQEYFEFIYISNQIYQSFLVEIEKIQLVDKIQFNEDIQFKDGIQITIQNYINEIQDSTKWLFQFIIKIEDQIKNPNREMRIDFKKLIAFPQSFYSIIEQLITKKENQYYKQESERYLQQQSLQINKELEKYEIPIQSAISILSMRFNDSILNDNDFSQIDNDKNLSGELTSLDDLQKLQMNCLKCDLILKYKRKHKTNQKNKFAQCSNQLEKINLNDSQYLMDILQAEYKTLEVIFLKKNNIEKVKELFEEFQALIQKNQISISHKQIVDDQKTRLKEFYRQVSEKVQQIIHSQVIIYSFDQLKPDLDELKNLYLDILQKQSIEDLKIKVEDTLNSKILKSFKENLNSQQQEISTTIKNYNNQISSLSNLMQKVNNHIYNFKHIDNQDQKNKYLKEASLDIKNYIQNQKLNKLRENEQMMEIPNSNKQKLDNFVENITLDQLNKIDLINYIQPQKKKLNQIGQPGIHNINLKKKGIISEINQIINQNYILTSQEIINNNNLSALEYMSNKFLINQVLQKNPVSQELLNNKQPDWIQAVKDLTKKNHIATLKGLLKDIKQLIADVNRNDRQGKELFEELNTFVDVMQNKRNQNVNQQQNVEIQMTQYKDCILKYKQEEQNKQYHLDNCQFKINPIYVEQRFKIKKFDQNRKIQQEFLFTNQQRNFDHECLSELTEFIQNTNGWSEVFLLFGPQGSGKSRELRILQNKLWQEYNQSKWIPIFVSFSQNSNLEHVFLKKLNGIFKDKNLLRQFQSAVNSKVEKVLFLIDDIDEVQIDKLKQNLIKLNFKHLGFTENLIGKTVKFIVSARSQLKDYYDYQLYFAYTNINTIIEIELLPFNDEQRLKYFQLNHQIRIKKVLFNYYIKYNKNKDINEFENIWKPLELDTQKLINEQEKNQVDYPRDSIPIYQDFQQAIIQSHLIENIKFDQYDKIAKDISKLRSSRNYLNIIQNHHFQHLTQSPFQLEIFILLFSKLRKQHKDLYFKEQIKQQYSKLQQKYPQSSKQTQNFSTSSLSNLISLKPINVEENLQKIIVQLEKQNFFSQYKYFNILRGDNRGIQMNSNVYTIKANSIIVIEALRANNLTMFQILQIYVASFNKKYTKKLENEIESYEFDSVFSKVEKFCEQLALQMTFNNLTQIEFDQQNLNADDIDQQQFLDEKIYINPFLINKQGNKYIFKYKMIQEYYFAKFILKFFTNEVDINEISQSELNKENVNLTFSNFAGALNIVRDHLRKQEKIREKLVQIIKMSKNSQYIRISSNLIYLMSYLDFNLEGEDLQNIQLSDTNISGISFFNCNLSKSKFTNVIINRCNFDFAQLNDVQWSNIQITKIANLDHEENVEQVIFSPDGKQLISLSAFWLKIWDFKSFDLIEKIENLDKNNHFLSFAISNKQSSNYYIATCSQNEVVLREFSNLNNILFQQLIDFPPAGPLQFSPDRKIMGFIDTYGWLRYWDNDLRQPLQPKYIGVEKIIENSPQLFYQINQIAFDSTNSYFLFKGQNTLTLYELLSEKIEQIKQITGTQFIVSSDGKSLAVAIKDEVSFYEWKDDNFNETLKIQFQNQILQILTLNSNQQIGLQFNDDLALWCIKDKCFKRIFKGFQNINNFCLNPQNNVIAQWTDTKKTIILWNMNQINPNQYYQIFDSEVLKAEFSPNGQLIILQLQSKIIFWDCWSDELINILDEQSLYSFSQNGNYLVTQSFKDGIILWDSSNIYELQQKAKIIDIYSIKKFFFLNNNNLETLAIISNDGELLKIWELKLILDQQQMINTNSEFDNIICIAVSPTDLNFAFVGDRQNNVIKISDREKWNINRELTGHNNKIIQIEYSGNGQLLASRSQDHYIRIWFVKESGANSIKLPIKYNPNLLDSQHICIWIPNTEQILASNEGDQIILWSILYSSYVKVLETQIQQPQQSMNINLQFEVYEKSQLFADECKDQECLQVSFSPNGKFIAALYFALNNQGFLNVLQNNLIEINPRIVIWNWQNKTVVQSFLIFSWQRFNFDNNSSSIITLDYDIENQTNILKFNPINQDVDPKQIQIGFQNHFSYLLFSPKSDLFLAVREEQNFINHFFLFKFQDQEQPTILDYFQLTYGLPVTFSPDGNFLVYPGRQTIEFKKIYNTNFPTKIRPLKSQYNWSQIKSFQFKYDQILFGFCETKNKFIISKNLETVEYNNEILEFDIRSVSFYDNMIACGGKSGSEIGEVQNKQGIVKIIDISNLDDLKQVQQIESLVSIMYVFFSHNGKYLLVQEQDYKITLFQQKDQQYQGVYLINLTIDISSVCFSSNENDFIILSNGGQVFFYNFTISLLSQQKDTNNTLSFLYRLKTIPKKYEIQAINCLINNSQITTEFNNLQHLFKYYGANQQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WJR2</accession>
    <name>A0A8S1WJR2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidylprolyl isomerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0910110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000091">
        <property type="protein sequence ID" value="CAD8188379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WJR2"/>
    <dbReference type="OrthoDB" id="199930at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050754">
        <property type="entry name" value="FKBP4/5/8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46512">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46512:SF9">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8188379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="311" mass="37155" checksum="C5AFBDB5A01DA2D2" modified="2022-10-12" version="1">MNQSDWFSIEDDQLFQNSEISQQNKTQTEMERIVSKYDKIILQQQNLIKYIVVVSKNEDPQKQINEKSRIKIRCEERDEKGTILNPTQNQNILKICLASSIPLIKGFRLALLSMKEGEKAWFKIQGDLLEKPDNIENFVKDQVKYYMIQVEEVIQPQQQLDVTNVENRVIQLEKFKQEGNKLYQNGDYVGAASRYLRGINFIEKWPKNLNKEKNSEVIKEDFYLVLISNRAQALIKLKDYQECQKILEPIMPRLEQKEYKVKCYYRLVFCLIQMEEYQKAQDYINIIKRDDNYFQYESLFKEFEDTCNQSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WKL8</accession>
    <name>A0A8S1WKL8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0950123</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000095">
        <property type="protein sequence ID" value="CAD8190083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WKL8"/>
    <dbReference type="OrthoDB" id="295138at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="55"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="358"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="232"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="286"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="413"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="363"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8190083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="707" mass="82599" checksum="592A0A98FE217B85" modified="2022-10-12" version="1">MKPYQTPNSGHSRQGSLRVQRQPLAVISNCPEFSQCANYLQQKVQGLEQRVKNLNSQHADRTENRSTTTQKNEASLSPFVRQKKQCSSNQKVDSQSYYETIINKRLENISKIDQSPVHIKTDNTMAQQDKYSMHSQFSNLGPQSGQLHTNSIVRIQQLTKKLRKFKMIKDQNQDECAPSLAKIKCFCQEYYNDCFDFYRCTDLIALKYFIYLLIQEVNSLYSQDAFKMADNSMQYQQQIEMLKEQLDNQKSDQMSFQNQLKLQKMVVDTQKLIQDIINVLQTKHNQSTLINQVEVLNNQMKNIKENMSSNQLQNQHQNQNATSFGQQLAKCGIDVNYKQRVTNDTSIVNQSNFKTQTADERNITQFSSKSKSPYSRNQKNHNFDLEQQMELNKKLFDEQVVTNRQLMEKLFKLQTEKQQNDQILKQVQEELKQKNQIIIDLENNLQKENQILNKINQNLEEAVYQNKELQKENNNYKENIDQSKKIEQKYYDLLQQNNNQLEQLNQVVKENGNLQKIQQQIEAEYKGYQQQILKLQEKCQAQYAQLQDQSDAKYLQQQINELLLQQQEQSKIITLITDEALYLGQMTLYASDILSRQPIDGIPTTLVMLQKDLNGKRSTIQQKMNILQQFGNNMRNQPLIAHSSSSQFNDNVDLLENLHSEASSIRQKNVSSYEETASTIHNNNIQSNDLMVMLLVQCNTLEKMMEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WMV9</accession>
    <name>A0A8S1WMV9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0940087</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WMV9"/>
    <dbReference type="OrthoDB" id="295536at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd11709">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050672">
        <property type="entry name" value="FBXO45-Fsn/SPSB_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12245">
        <property type="entry name" value="SPRY DOMAIN CONTAINING SOCS BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12245:SF5">
        <property type="entry name" value="SPRY DOMAIN-CONTAINING SOCS BOX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="1">
        <location>
            <begin position="135"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8189559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="349" mass="39960" checksum="810201E084B17152" modified="2022-10-12" version="1">MTDTGFECSLCIKLAIKAQICEKCGSMYCKKCLQEIFGKNYECVQCQSKQFRSIKKSALQEAYDEILIKSQIIQPKKIEKPPPKQPVIEQRTQQIYLNEKCINFEMCQNNINSIFINEKVCSLECLYFNKIQPLLEQQDFTKIRKEIINLEQSIKNKEPIQIFKKIPLSLSFNQVGVSNFNFDRCGQGITIKQSTIILQEDEYTFKTVTSSVGFQQGIHFWKIIPLAMTKNEMKIGISTSDKYDLKTAFSDYNFGYAYYTVGQFRNGSNSNGFEYGVKFKNNGELGILLDMNRGVLAFSYNGNFLGKAISSELLKKGPIYPCVALLHQAGFEFKCGIPIPQNILEQFQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WP31</accession>
    <name>A0A8S1WP31_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATP-grasp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0960090</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000096">
        <property type="protein sequence ID" value="CAD8190381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WP31"/>
    <dbReference type="OrthoDB" id="288171at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070740">
        <property type="term" value="F:tubulin-glutamic acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241:SF147">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE TTLL7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035855147" description="ATP-grasp domain-containing protein" evidence="5">
        <location>
            <begin position="21"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="6">
        <location>
            <begin position="418"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8190381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="537" mass="63839" checksum="D28E79615281F751" modified="2022-10-12" version="1">MTQQILLASLFILFLQKWLIENTLVYKQYDQVKDILKQMNNLTTSNFYNNRKYFSNKRSFRFDLSKSQIQLNETYCNQSDTFKFYHPDIVLKDIYTTRETDDLTQEIVKSISFIKIPNYTKDKQQNKTALPEALTIPFNYYDSYLTRSNTGLSAMCLFQKHNHMRGRQQLAYKNELLNNYYQYVDKLKTMPQICSQNVTYIPKSYRLWLKDECLAFFELINTPEYQQKFQKHGPQFISKLGLEVHRGAGITMLFEKETKQLIKEYQNGQNCGQISKYVIAQKYINNPFLFKGHKIEFRIYFIIASTYPLIVYAYDSSLIRRCAMPFDKFSLEKSSHVCNTAIVKKKLEGQDLQEDQSFFIEWKLDYLQELLLQKKAIKNKKWLNDYLYPKIHNTIQHLIMSTQHYFDNDSKLSEFFGVDFMITDDLQIYVLEVNSNPQLLKTTPGRILQNTQLVNDIIEIQVAYLRSKYVRLRSLIKRILSSKSTLQKFDKEFKEAYKDKLEPEFPISSNNLFRKIIDKNLKDSKAYNDMIQTKCIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WPT5</accession>
    <name>A0A8S1WPT5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0970113</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000097">
        <property type="protein sequence ID" value="CAD8190780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WPT5"/>
    <dbReference type="OrthoDB" id="296677at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06463">
        <property type="entry name" value="p23_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CS" evidence="2">
        <location>
            <begin position="116"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8190780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="305" mass="36354" checksum="30B5448D4F8037D0" modified="2022-10-12" version="1">MLFLTFLISVSIASQDLLKKCYLEQFTIGDPEVKIQIYFEDHVVKNHQIEYECLEFIISRGYYKVALSLYENYFLLNHIDITDRIVQFLKNDKYLNQREMQTLFKLAMAKSNQVQVVQPVVQWAQSKNATFINIKFSHRQDAPACLNAKLEVVEIKNDSLLIEAFGIVSHIPFKYRYAIKLYKPIDPATSYEKVESVGTMYVNLTKIEPVLWLRLTEEDYKTPIWWDLKDNFRKDMEEFAQMLEKESERKERNADKQAKKNQKKRDQEKQKQTSQKAQEAKRQLEYEHNQCYKPGKCEIGWYQRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WSM0</accession>
    <name>A0A8S1WSM0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1020135</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000102">
        <property type="protein sequence ID" value="CAD8192723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WSM0"/>
    <dbReference type="OrthoDB" id="285911at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8192723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="204" mass="24052" checksum="7ACA94831D83FCCD" modified="2022-10-12" version="1">MQELEEFLEKQKDLNIKMMRDMLQTQSLIQHNLSIGLDSLKKCIENLPNKIVPKQGLQSRITIQRQNQHQFILSQLGHKNFISYLNLEEEIKEIYRERMFNLSVSMRDMNGDLIEDEDCPIFLRIYTSEKFPKEVYSNIRGKPIFKGQTQILLRGKGEFQRISITEVSSHFPSGKFLLVIFSEKQYVKPYIIDNLIVKAKKLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WUN3</accession>
    <name>A0A8S1WUN3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1040118</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WUN3"/>
    <dbReference type="OrthoDB" id="286605at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8193503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="189" mass="23375" checksum="4A0079F782C46B70" modified="2022-10-12" version="1">MQIEQLEEEEHSIQALRAKYKAMISVQILDIVIRCFIGSMWALITYSSILGFFKLFRIVYNQYKYNYYKDKWIKEVSLYEDEDCINETNEKYKKIIIHYHDRLEKYDQKLQKGLYRYSFLIVLVFGMNIAWWFFDYMRSCMGYGFVVFIIYQTYCFLDRKYIQPYFERREHEKEKQRLDAEIAKNKKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WVW4</accession>
    <name>A0A8S1WVW4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1060066</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WVW4"/>
    <dbReference type="OrthoDB" id="291270at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8194184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="248" mass="29516" checksum="988E9026FA313A16" modified="2022-10-12" version="1">MNEEIRTILKKILEMQLDCTQMLTTILFKNDDSYKDEELNTAKILLEEQKNQIELGDKKQKKKHINHDKSGEVVNQIISDHHQNVIIDNNTQLKLTPQEGYQKLNHIIQEFRQRNKLTIKPQSCDFEYIKNYHSKNKFKELRNMIKSVVNRIENKKMVISKANGQKVIQCPSCDFQGFSGVYFPHILKKHCQNYYIFSCFLCFRDFQSYILLKKHLKRYHKQVLNNSSDIEIIKIQYDDNDNDLSINI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WXL9</accession>
    <name>A0A8S1WXL9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutaredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1070102</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaredoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000107">
        <property type="protein sequence ID" value="CAD8194668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WXL9"/>
    <dbReference type="OrthoDB" id="423541at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02066">
        <property type="entry name" value="GRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46679">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46679:SF1">
        <property type="entry name" value="GLUTAREDOXIN-2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="6">
        <location>
            <begin position="12"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8194668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="94" mass="11205" checksum="5FE43EA0D1209FAE" modified="2022-10-12" version="1">MEHQEIKSDKPVVIYGGDYCPYCHKLKHFLDTKNIPYEYRDITKDKEHERQVNEFVVKLKWSTIPMVFIKQKFVGGYTDVVNLDQKGELEKLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WZ86</accession>
    <name>A0A8S1WZ86_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1090023</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000109">
        <property type="protein sequence ID" value="CAD8195264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WZ86"/>
    <dbReference type="OrthoDB" id="306282at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8195264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="179" mass="21299" checksum="16F7873AA4EE900C" modified="2022-10-12" version="1">MLGTPSLIDFFNQDSFVENNQMDLENIDELESQFNYEYTKQPTTTTRHDTIKINEKDINYKNLPKMIGNNFVKWIKNYKYSSDFDKIPKGIKKLVELREKERQSKIKIKDLRESIINDEDSQIIFKEYIADQLYLDLLFSNKVADPFSYIPGISNYYSAANEPEKMKSNYIMRKEFINQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WZ91</accession>
    <name>A0A8S1WZ91_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1040154</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WZ91"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8193565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="359" mass="42992" checksum="3CD2B78CD8604DFA" modified="2022-10-12" version="1">MQQREKLFIICGNINYSTNLMPFMKEVIIQKNNRIKGSQESKTDFILVYYNAQVHLNVYVTCKIDHFIFEWQKFNYQITSITNSTNSKKYLFIFFNSIVNEKRFLNRQHKLQKELVNTITDHQILLYKILNNKSEVINRKKVFESINRILIMKPSKKEDSDIMNCFIDCELLISCNKYILQNCLQQIAIFQQIQKNSQLKLFKLNPISPEECQLMKQRLSQNKGLNFYLIYDKWIKQDQTYECFPIYDLKRMQIQFQTLAKIANSIKLSIPKHSRNRLIQTNNNQMSNYEMTRAQIPTQNSNNSVKITSLKNNWFRLRCWNQTVYLNSQLIRKILVKQIREQLYFQSAQNCTDLSILFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X1P9</accession>
    <name>A0A8S1X1P9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1120042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000112">
        <property type="protein sequence ID" value="CAD8196268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X1P9"/>
    <dbReference type="OrthoDB" id="4869960at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="32"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8196268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="254" mass="30246" checksum="A60C714E152FAB35" modified="2022-10-12" version="1">MKQSKQFISGDWGGFIFIWSFIDNVWIRSQVIQRHSNPINCLILNNNEDLFISCSDDKTIRFWAKKNEQNNEWICQQIISDHIRWVYQIILNEQENQVISSGKDGLILVIEYSESNKIWVVIQKIVENCRGFRIGFINDNLFTFKPIDGNFMHVYEKNSVSKEFTRNKDIILTQGDDGYGLFPQQFIKQKQLIVCKHDKYINLIRNAQNGEFKVEQYIEFNTYYIYGQMSDDGEYLITWDNSSNEIQIRRYIEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X518</accession>
    <name>A0A8S1X518_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Calcium-dependent protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T1140109</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000114">
        <property type="protein sequence ID" value="CAD8197093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X518"/>
    <dbReference type="OrthoDB" id="306407at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:002767">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="80"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="362"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8197093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="496" mass="57733" checksum="C40703404F95004D" modified="2022-10-12" version="1">MGNCSTKKRVKKQSEVVVSTEGPDEKTRIDRKQQKSQTLTPILAKTFLSDTVNESVISIRSTKKILDQQKQPIQIRSGLFRVYTLLGGRPDSFLEKLDDKQIRIVQHNISGSKKRVETINKSDTSTQNFILKIQKNRLYNEYICPITNIYEDTDKYYLLSDYCSGGSLNSLKGKLKDNQVTILLNQMVTAISYLHSKKMVHGKLSLDSFHLLNDLNSLFCKLIDATCLFQGKEQVKIEPSQEDYAEDIYALGMIAYQLLTGLMPYQIKINKQQENNENALVLLYFNEGTSPSLKQILKKMLEKKISERITIEELKRQLQKNDIRNNYSDFLIKPLYLLSKCKQRNYFHVVILAFMLNKFNQEEECILEKIFNDADLDQDGLLKKEDIIRLYKSIVEYESINIDIQQLFQKLDVTMKEALDIKEFVSAALNLEDLLSQTYLETCFKYLQNQRGYITCNSVKKHIEINEELFIQTLEDIKGQQKVNYNEFTEIMRQLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X6J6</accession>
    <name>A0A8S1X6J6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1060118</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X6J6"/>
    <dbReference type="OrthoDB" id="18915at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071108">
        <property type="term" value="P:protein K48-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22749">
        <property type="entry name" value="Otubain_C65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019400">
        <property type="entry name" value="Peptidase_C65_otubain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12931:SF15">
        <property type="entry name" value="UBIQUITIN THIOESTERASE OTUBAIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12931">
        <property type="entry name" value="UBIQUITIN THIOLESTERASE PROTEIN OTUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10275">
        <property type="entry name" value="Peptidase_C65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8194286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="309" mass="37574" checksum="7BD5B69053894D4B" modified="2022-10-12" version="1">MSQQSSNQYAYNQELYEQHIKQLDKIKNQIEAQDQFIITQPIEPQILESEYLENQGFLKKVNKISQSYPFFRRMRRDGSCFYRAVLFRIFEYIFNQKISKYQLNLADLTAVGYELIVIDDFYDDIMKQIKLCPNNINKQGIVDAFCNKVTSDYLIMCMKMMTSFYIKANSFIQEVTQKQEQLQYFVNKKLIQLIRKQIKFKSYHCITTYKFQLGCSIWMGILPSSMPLFFKFQKMQIQILYLLIDYKDLDIMTFFIQNDVIYYHFHFYNKIIILIFHLIFLCWLLQYNVLRDQFIFFEQRNLFYFLVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X741</accession>
    <name>A0A8S1X741_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1130064</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000113">
        <property type="protein sequence ID" value="CAD8196629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X741"/>
    <dbReference type="OrthoDB" id="124041at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033204">
        <property type="term" value="F:ribonuclease P RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016848">
        <property type="entry name" value="RNase_P/MRP_Rpp29-subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002730">
        <property type="entry name" value="Rpp29/RNP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13348:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13348">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01868">
        <property type="entry name" value="RNase_P-MRP_p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00538">
        <property type="entry name" value="POP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8196629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="271" mass="31885" checksum="8A74FD68424D0758" modified="2022-10-12" version="1">MDLDEIFGKIENKEKNQKGKQVQKVDKQQKSIQNREQHQLKMKLNHEINRLELVKLTAENIYQQQINKGSADQFQNEMIDLQIVSMIGDPDIYGDKIKHKNFLIGKFEKPFQLKNTADNELQQPLKNGLNRQLKIFKRSLGNLRYAAVTKLNSLWKLYIEDLLCREQSADSKFQRLLKADFHGALIQIVYSKCKTYEGQEGIIVREKKKSFIIIKENDQLSVIEKENTIFLMPVGNKTYRLHGCHLIIKPSERIRKDIKYHPANVFCYQYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XCB4</accession>
    <name>A0A8S1XCB4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1190085</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000119">
        <property type="protein sequence ID" value="CAD8198608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XCB4"/>
    <dbReference type="OrthoDB" id="307514at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051948">
        <property type="entry name" value="Hsp70_co-chaperone_J-domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44360">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44360:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="19"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8198608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="252" mass="31437" checksum="364E99453955D68E" modified="2022-10-12" version="1">MYYKQKYFFSFLKFNPNKNYYQTLNLTNRATQDEIKSAFYTLAKLYHPDKNPNSIDQFKAINEAYEVLKNPTTKKDYDQIQINPVKYQKQQYERKYYQQSSYQEYNQNQEQFRKSYEEYQKQQAFKQYHFYSRTGYDYQDPFKDYSKYMRDEQYDNYSNRNPAPRYLIIFMMTTSAFLIFELLDLFANNKKVILTDQITGQQYITTRQELRNKSYINREQQEFERRKTEYKIMQQEEIRRIRQRKFEQIIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XHD6</accession>
    <name>A0A8S1XHD6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1250129</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000125">
        <property type="protein sequence ID" value="CAD8200780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XHD6"/>
    <dbReference type="OrthoDB" id="305586at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="224"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="7"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="238"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="267"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8200780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="299" mass="35882" checksum="5E5EEA93AFD1FAD0" modified="2022-10-12" version="1">MQDSQKLKDLQIQLNKFICRLEELERENKYLKEQLLNQQCQLMQNVKIIDNKYQQMHRKYLKCLYKYGIQQSCLTSMIQQYNKLNGIEKMDLKQCIERFGVIYEKDKIINEEEMKYIIKERISKDSEEIYTLFFPTKIIRNECLSENDSLSNTCTFQSQRCKIQSSLQTNSDINTDSLEETIINNLTTQRYLEKLQFDYQNDYTLHYPKAFKVEQLLQQSNRDNQILTDRSLKKKQERKGGCKGEQNHSSRIDGKVSPLKQLNSSINEDRMRRFDTHQENDISQNRRRSRAERLKSQHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XJ27</accession>
    <name>A0A8S1XJ27_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1260184</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000126">
        <property type="protein sequence ID" value="CAD8201171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XJ27"/>
    <dbReference type="OrthoDB" id="310721at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050498">
        <property type="entry name" value="Ycf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE SPINDLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="50"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="152"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="186"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="220"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="254"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="288"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="322"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="356"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="390"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="424"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="458"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="492"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="526"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="560"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="594"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8201171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="676" mass="79097" checksum="0B5108BF0E08E509" modified="2022-10-12" version="1">MGSNIQKEKDNDSASAIKYYTRSNELKIAYRTEEAILEINKALNLNPKYAEAYTLRSELYYKMELYDKALQDVNFSLSINNNNAESYYQRATIYWRQKQLNLALKDCWKCVQINKNFAMGYCRLGTIMWDLKEIDKELEYYNMAIQKDSNCYNAFICRGCFHMDGQKADLALRDFNQAIQINPQFSLAFYNRGYFYYQIGEQDKALNDYTKAIQLDPNNVITYRNRGFLYSNMGDKEKALNDYNKAIQLDPNYAIIFNNRGHLYSIIGEKEKALNDYNKAIQLDPNNVITYRNRGHLYQDIGEIDKALNDYNKAIQLDPNNVITYRNRGHLYQDIGEIDKALNDYNKAIQLDPNNVITYRNRGHLYQDIGEIDKALNDYNKAIQLDPNNVITYRNRGHLYQDIGEIDKALNDYNKAIQLDPNNVITYRNRGHLYQDIGEIDKALNDYNKAIQLDPNNVITYRNRGHLYQDIGEIDKALNDYNKAIQLDPNNVITYRNRGHLYQDIGEIDKALNDYNKAIQLDPNNVITYRNRGHLYQDIGEIDKALNDYNKAIQLDPNYAIIFYNRAILYKHIGKHDEALNDYNQVFQLDPNFVQAFFGKGLLLQELKQYEQAIICFDKAISLDQNHINAYFNKAISLEQLNQFFSAIKYYKIALTLNHPSQNQISLQVSILREFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XLU5</accession>
    <name>A0A8S1XLU5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1280110</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000128">
        <property type="protein sequence ID" value="CAD8201628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XLU5"/>
    <dbReference type="OrthoDB" id="298858at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049680">
        <property type="entry name" value="FLVCR1-2_SLC49-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10924">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10924:SF6">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 49 MEMBER A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="262"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="297"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="331"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="353"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="386"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="421"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8201628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="527" mass="59035" checksum="B9F6F64051B188DE" modified="2022-10-12" version="1">MSNHQSEADQKKVKSISDHSLVKSHSQSIKDIELSVYRWAILLLYYLVVLVNGVPYEICVPISSFMAEIYNVSLAVVATASTVFMVMHPILSFVAAQTISNYGWASATNLGVILTILGCAIKLLINQAGFTVLIIGQVLCGIGRPFILNSQASMAMSWFHPKTRIMIITLLNVVNTLSLVVSAQLPGIIFKGYKLNDDTEYEDGKEKMQSLCLLEFYVSLAIVPSLFLLRSRPSQVPVKINKIERKSGMLKIMLRLFKNRNFVLLFIPFSLYFGILKAYLVIMELLMAPYKYETDQVAGVVSFPLIGGLIAQGALPYFVNKYQIQKPFVRFFLLFSTLSMASLYFALKSSNVVYMYIVITLLAMCVMPILPVIMDMGCDLISPIEPSFAVGAFYMGSMLFFVAFTYILTFITGKNKEDTRVLYTNIFSTIMLIIGFICSLFIKIDDQYYELNVEQDKGSLVHPLEKSQSIQKGKGPSVYRGTQSKMSVQYQKGSMFSCSYNNISIVGDVPLIPGEEAEDPIDQEQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XNG4</accession>
    <name>A0A8S1XNG4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1310088</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000131">
        <property type="protein sequence ID" value="CAD8202537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XNG4"/>
    <dbReference type="OrthoDB" id="5337378at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="15"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8202537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="609" mass="70747" checksum="1EDF505C4BB449E4" modified="2022-10-12" version="1">MKKEEDIIKVQSYEIYKNQEIGRGQFGVVYQCKDLKNPQLKLCAKVVEEKLDDPKTIREIELMKMIMICAKGNKNIVGVEYVDLNVERIILILEKCESDLQSIIDKKRKEEQKYFKPSEALNILKQLVNGYKILYQNNIIHRDLKPANILVLNGIYKIADLGLARVFEGNTELTKVGTPKYVAPQLYLDNRFSNSADIFSLGIIIYELIFGGLPYVANTQLQIKKALRNLEKVPVTVNREWPEMTYEFADLIEQMLKFHEKDRVSWEELFKHPLIQEESNLVVNQSGLISNNLDSDEEEQEEEPVQVQKPVEKQNNVQQISPSIPNQAQQTFPVPISMTQKQQCFINPQTQPQFTTGKVFTAPFNPNSSIPTGNPPNPNIPNPNSMIQSAQPRPFINPPTQRLQTIPTMHILGQPIQFQQVKAVSDQLFQAGFVLDQTLESLEKFLNMQLTLKQEFFGIKLQLHCLSQCFYQHSSQMISQNQKNPNFQSFYSLQIVDSEIQKKIKYHQVITQEIEKYNLTSFYPVSAINPDFVSYIQKLNRITQVRGVFDYQNDRKYFQDYLSMLYFLERLKEVQQPSFTDLYNLISDIQKNKKIEQLVTDYLNKRLCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XT88</accession>
    <name>A0A8S1XT88_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Developmentally-regulated GTP-binding protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Translation factor GTPase DRG1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T1350034</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000135">
        <property type="protein sequence ID" value="CAD8203792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XT88"/>
    <dbReference type="OrthoDB" id="603at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008092">
        <property type="term" value="F:cytoskeletal protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031116">
        <property type="term" value="P:positive regulation of microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01896">
        <property type="entry name" value="DRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17230">
        <property type="entry name" value="TGS_DRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000003">
        <property type="entry name" value="Developmentally-regulated GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002634">
        <property type="entry name" value="Small GTP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045001">
        <property type="entry name" value="DRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031662">
        <property type="entry name" value="GTP-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006074">
        <property type="entry name" value="GTP1-OBG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43127">
        <property type="entry name" value="DEVELOPMENTALLY-REGULATED GTP-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16897">
        <property type="entry name" value="MMR_HSR1_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00905">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="12">
        <location>
            <begin position="64"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="13">
        <location>
            <begin position="289"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00072736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00080795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8203792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="367" mass="41546" checksum="44D9E02805F8E577" modified="2022-10-12" version="1">MTTAQKIAEIEAEMMRTQKNKATSYHLGLLKAKLAKLKREMIDSSSKKGGGTQEGFEVSKSGDSRIGMIGFPSVGKSTLLTKLTGVFSKIAAYEFTTLTCIPGVLQHKGAKIQLLDLPGIIEGAKDGKGRGKQVIAVARTCNLILIVLDATRPMVHKKIIERELEGFGIRLNKQPPQIEFKKRDKGGITISRTHDAPRLDDETIKAILREYKINNCDINFKCDATDEDLIDIIEGNRKYIPCLYVMNKIDDITLEELNVLDKIPHYVPISAFLEWNLDELVERIWDYLDLIRIYTKPKGLEPDYNAPVILQRKFSTITDFCNKLHKNMIKDFRYALVWGASVKYNPQKVGKDHQLLDEDVVQIVKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XUA5</accession>
    <name>A0A8S1XUA5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Band 7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1360042</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000136">
        <property type="protein sequence ID" value="CAD8204138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XUA5"/>
    <dbReference type="OrthoDB" id="287724at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13437">
        <property type="entry name" value="SPFH_alloslipin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000004">
        <property type="entry name" value="Putative membrane protease family, stomatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043202">
        <property type="entry name" value="Band-7_stomatin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264:SF19">
        <property type="entry name" value="AT06885P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264">
        <property type="entry name" value="BAND 7 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Band 7" evidence="4">
        <location>
            <begin position="82"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="188"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8204138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="302" mass="34400" checksum="9C8A18CC1C4B869A" modified="2022-10-12" version="1">MYQQNQVMYQQHPNQQIPVQQHRDYNVPPPGYKAPRYPREELPENQEQNLDLGGSYQSYLYNCGECCGNCKAVCPCNPFVEYPYVQVEQSFVGVYLRFGKYIKTVQPGLIYINPCTDTIQKVDCKVQMIDCPRQQVMTKDNILVSIDATVYYRIVIPRRSIFYINDLHQAVTQLTLATIKSIAGSHTLQDLLEKRAEVQQQIEGFVDEHVWEWGIDIENMLIKDIQLNADLQNTLSMAAKEQRAAQAKVISAQGDVQSAKLMRQAAELLDSKAAMQIRYLDTITTLGQQGSTKVVLLPTDSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XVV5</accession>
    <name>A0A8S1XVV5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1380012</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000138">
        <property type="protein sequence ID" value="CAD8204628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XVV5"/>
    <dbReference type="OrthoDB" id="295180at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="505"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="450"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8204628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="719" mass="85255" checksum="BD0998A60137A52C" modified="2022-10-12" version="1">MLQIKVSSSLPQNPYLINQVEQFLIDSNTLIRNGYYRKCIIHLDGQYKKLLPIESPLLLRIKVLRRLCYCTLMYFKLKINKAEINQGKNQFTLWTRFQLYMRDFHDNISLLKIQYKKKFYTQELISIMIKALLFRSQYYQKNQMVAKGLLYFHHFNGTIIEQALIDKITFFLSLQGKYHLITGNSYFQLQNYQVALKYYLKGLDFYQRNLIIIYKEITLTSLEEIKMTLNYYTKEIILSLFLIALVYEQLNDFLKYQETIKMMTWVDSNYQPEDSIGKLYFQQYVDNTRYLEYALEKAEISKVLKQALPEEETSKVPLGEDQLNYYNNLLYEFSDFPKIRKQYYYYDKSQIPYSNETIDSETYLEKQKTLKTNASVQMIYKQQTNENESTTMCRSALMKTKYSDNGLSTRFQTESEGFSQPLIHNQQTNKKKKKQLNKEDITLQMYYGNAIEKSVKLQELENLAEQHNQDLAQQQIDTISFDKKLKQSKQQIQFKKMFGCINLNKGPFEHETRESRKRQRKQQAKSINQLQQLLSIQLQIKTKTEQSQLLEQYNKQRKVNCSSQPNIFQKVHNIHRQNKTVRIESVITNKEFSKDMSSHHKTQDEDQLIREININTKETIKQMMDEKESLIQSKPFSTRRSSQNNTIKNNFLFEKLILKNDSLAHTTDQISNSKKLNSLKSLLEVPRNNPNQKRMTAILSTITPRSASKMGSHTILPIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XX91</accession>
    <name>A0A8S1XX91_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1410104</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000141">
        <property type="protein sequence ID" value="CAD8205687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XX91"/>
    <dbReference type="OrthoDB" id="304160at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="18"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="175"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="33"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8205687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="247" mass="28778" checksum="13A9BBC08CFA223E" modified="2022-10-12" version="1">MDPKRQIQQQQELSLVHSSYEDEQGNCSLKDSYSNRDSDSSFSLSEHEKRYKKILTHSLNEQEITKRKGGKHGLKYAKISNDQREGLIQQVTSTGCTIKSAAQKLGINFSTAKAIMQIFKKEGRSCKKVIRKNKKRQSQMYKRHYAVTKDQSIREEKEQLRQTGEIAKETIQQPISVLEDKNKEQNVIIQQLRSQNLLYQGQVANLYQENAVLNQNYQNLYQQYQHLQSMMQQIFMKTQSPLAPFII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XXX4</accession>
    <name>A0A8S1XXX4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1400006</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="EMBL" id="CAJJDO010000140">
        <property type="protein sequence ID" value="CAD8205172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XXX4"/>
    <dbReference type="OrthoDB" id="298128at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="57"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="234"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="8">
        <location>
            <begin position="147"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8205172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="326" mass="38271" checksum="B8EC76DA27C75DDC" modified="2022-10-12" version="1">MQQLQEDYQSNQNKINKKYSLNRILSCFSKLLIFFCYLLITTILYSFIFDTAMQYHLVYSNTILTIAILLLGLLFGFNVILNYTFVIQVSPGTTNDYFIPIQNDTENNENQIENNANPHLKYLRFNYAPKLEVNTSSPSAILDQSTLGFCNQCQLPKPKRTHHCSICNKCVLKMDHHCPWINNCVGHQNHRYFVLFLTYIFLGTSFITLLNLNIIISTDFEEFKNQRSSLFSTIWIIELALSWSMACFGGWNWFLVLRGFTAIEFMDRNRKTTYEKEEIIENLKQVFGDFKYLFQIFLPSFRQLPSNGIIWNYSEKKLEKINIAVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y3G1</accession>
    <name>A0A8S1Y3G1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Calcium-dependent protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1510086</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000151">
        <property type="protein sequence ID" value="CAD8208606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y3G1"/>
    <dbReference type="OrthoDB" id="304933at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="137"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="436"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8208606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="566" mass="65452" checksum="30C461CD71BCC59D" modified="2022-10-12" version="1">MGVCTSSSTKNNQKQKKLQPETQANQQISLVSAQKNNTNQLEELNLNKKALTAQRNHSPNKTTTPVQINSIKNGTMSIRRASHATTTTTKTLIVQPSLLQEQQQIKRSSLLNVPYKRNSIRQGTKTLQVSASRNYSIVSSKNLAENLGKTVMQHNETGQLVQVELLKFDSKNHQYIEKLEEIKLVSRLIIQDNMHIVKIIDVLVDHQKKTYQIMYECCSGGSLSKYIENRKYDDQQIGIIFYQMIEALAYIHSLGLSHDELTIDSFSIFDDSSTPFIKLSNIRSIYQLIYPKNAHLYEDPLQSKHQNSNNKYNDRNPIEIKTKNQCSDVWALAIIILQLKNKELPYDISEIHSFKPEYYLNKYPSEMNSLLLEMLHINRHDRISLKQCLEHPFLIKMKQVQPQDLLQPLKNMIKCKNMTYLHKCIFQYLLQNYANDHLKVLTKLFITADINSDGSLSLEELKELFSLEGKDLIDQLNLQKDLTLDEFLLSASNKDIILTQDIMESSFKILSRQSNLITPKSLYKVIDNINELQILQDFKSFNLNDGLNLQEYIEFIFNYETPIPID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y4X5</accession>
    <name>A0A8S1Y4X5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mitochondrial cardiolipin hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1450074</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the phospholipase D family. MitoPLD/Zucchini subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000145">
        <property type="protein sequence ID" value="CAD8206824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y4X5"/>
    <dbReference type="OrthoDB" id="5205528at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016891">
        <property type="term" value="F:RNA endonuclease activity producing 5'-phosphomonoesters, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000078">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051406">
        <property type="entry name" value="PLD_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43856">
        <property type="entry name" value="CARDIOLIPIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43856:SF1">
        <property type="entry name" value="MITOCHONDRIAL CARDIOLIPIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phospholipase D-like" evidence="6">
        <location>
            <begin position="39"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8206824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="206" mass="24594" checksum="1B8F716827C36B0F" modified="2022-10-12" version="1">MNNQLFTYGKNSIRKQNLEKRKLQTIFFPNKWNFHYFFNLMKDCQQFIIACVSEIDHSWIIKLLVLLANGGRKIYLIMDCKENQKNDEQFQAVICELLIESQFRIKIVVNTKKYENLPTNFCVIDGKVLITTSANWTINSFNKSHEWMMLDKKYDNIVEMIEIFEQMWNQFKFVTFINEDIALLDNEDSQFQSVFTYSLDCIVDTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8T7</accession>
    <name>A0A8S1Y8T7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphodiesterase</fullName>
            <ecNumber evidence="1">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1580035</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="1">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000158">
        <property type="protein sequence ID" value="CAD8210159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y8T7"/>
    <dbReference type="OrthoDB" id="189220at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="3">
        <location>
            <begin position="743"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="709"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="713"/>
            <end position="725"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8210159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1089" mass="127667" checksum="4FAF282B4214A711" modified="2022-10-12" version="1">MVKAVEYSQFKCLFTSVNYFETIVQNIKMDPQLSHYTQFFIVVTNLCKTNQITPEQKSLLKSNLTSKDEKICRMLIQNSGPGKEIQLREALLDYLAAQNKQIQRRRSHKSKTVHFMKDVAEQETQQEIVPQQESSAQPLASAAAVMIEQLVGVLHKHIDKHSLLVSEDKEKLIQLCNLIQKLTSEQVDLDGVSPFRARYSSENSKLAEISEKDLDSSDEDVYEKMVKGIDHIYKELKSIFTGNLHTHLLLMQEDISYENLYQVLKFLLKTLVDADEFTFFIHRDKEWEVYSSSNDSIKDVTPEEAQRVTDELAYIRPFCIHRIDPKQRQYPQIEETCKTNTFSQTSIVKFQLQLKNYEVLFCLHWTDKDKKNIKRKFINYANMYGFNKDVTHLAQFLVETIITAKVQFFNPLRFSDQVQDIGISFMRISRFLLVEGIKKVLSIKFEVEKEQTFSTDENIKRQKESQCVKIELKDQNPVTLRIKDMDLKNEQDLHLYSAIIQIQEKYDGYVKLCYEKSAFYKYFLRTTDSLLFDFNKQGELIFLSRPISKSLKERFNINFDPKAILYNKISYQDIFAQNSIISNIEVNIQNIHENRRNQYLSNLENPQFEIFLKVVDNDFKGFTVIFHENEARRLKQYFMALKIDNMTNDVQKEAEANKSLEEDIQKQILIQYNKHQTFKFLNQLDETQDVANSMIALFIPENELQQIRHRKTDGRSPEQQSKDLQNDQWVAPPQKKKKIGSSVYIKYQQQLETVDANLFKVNDSDSQLDLFEFNILVLDSSQEKHRLVYSILERNGFISQYKLNKQCLAQFLSVLQKKYNKRNNSFHNYDHGIAVMQSAHYMLQCGKAKQQIDDFRRMSTIISGLCHDVSHTGRTNIFMINSRSKLATRYHDSSPLEQHHAATTIFMLKDPSLNFLNSLSKEQSQQFRRILIDNILYTDIKVHFTLLKDFESRIKDEVTKPFGTGDDDLKLLTGMIIHTADFNGGAKVFEISRIWSERVNKEFSAQYDEEGRLGIPQTPFLKDLDKLHIMAKSEMGFFKVIVRPLWFTLNTFFDGYLQQSITNLDNTIISWEKIYHANLPKEEKQQQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YEX9</accession>
    <name>A0A8S1YEX9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T1720073</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000172">
        <property type="protein sequence ID" value="CAD8213006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YEX9"/>
    <dbReference type="OrthoDB" id="296179at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05580">
        <property type="entry name" value="STKc_PKA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000005">
        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF37">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT PRKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="16"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="10">
        <location>
            <begin position="271"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="296"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="296"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8213006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="323" mass="36693" checksum="28A1EEB65C448137" modified="2022-10-12" version="1">MSTSLTKPKKLKLTDYEVFQTLGTGSFGRVKLARNKQTNKYVALKSLKKAEIIRLKQVDHVINENTILGNLQHPFIVNFEGFCQDPRYIYLVLEFVSGGELFTYLRSIGRLDTQHAGFYASQVASIFEYLHSKNIIYRDLKPENLLIADDGYLKLTDFGFAKVVEGRTYTLCGTPEYLAPEILLNKGHGKAVDWWTLGILIYEMNAGIDPFSDEDPMAIYQKILKGKVKFPKSFDKNAKSLVKHLLVADLSKRYGNLKNGAADVKNHRWFGNLDWNLLTQKKLPVPYKPVVKAPNDTSNFSSYPESDTQSPALKPADDPFLEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YGW0</accession>
    <name>A0A8S1YGW0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1700005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000170">
        <property type="protein sequence ID" value="CAD8212628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YGW0"/>
    <dbReference type="OrthoDB" id="318830at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8212628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="249" mass="29473" checksum="733B2B3D7ACF1C78" modified="2022-10-12" version="1">MSILQTDSEKISCKAHNNEYCVAADLNTKGQRDVNFLCIKCIIEQLSNKKITLIDDTKRLICEMKTQKIENQQKEKEKRYFLDIIKQIVTVDVAFDKMTSFIQQRLFYIQKEIESLQYEGKNNYLEDLTELSRIYSEEKFDNGIKIQEDNKIIEEIEKQFETLFNTPQYLQLNQTIKNTRSLLQETTEATVLIYLIQPSNEKLVYLLMTQKTPSLKKLCKIHKKEIIMFDADVESKIRKQRKNLCVYIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YI05</accession>
    <name>A0A8S1YI05_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1740015</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000174">
        <property type="protein sequence ID" value="CAD8213113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YI05"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="317" mass="36824" checksum="17AF96F112DF9329" modified="2022-10-12" version="1">MKCLSQVNVKAFSYQIIEANSIKQQEYCWAVAINKDCSIVAAGCNKLIKIYEFQQGMLKLNQVLNQHKNNVFTLIFMKQQNQLISGDQRLILICNWIRCIILNNNEDLFISSSDDKTIKFWVKKNQWIFQQSITDHSSYVNQLSLNEQQNQVISCGYDGLILVIEQSETNKKWIVKQKIQVDSIGYRLCFINNNLFTFQPINGNLMYLYQINSVSKQFTKSKDITINQGNEGSEFFSFANIIIISLETSLEITQITKKDIVQSKNNYFERIISKVVFMLSLNIQNLVNYCLDISEINIQSVKLYFIPIQFVSFQNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YM60</accession>
    <name>A0A8S1YM60_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1670010</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>tRNA(His) + L-histidine + ATP = L-histidyl-tRNA(His) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17313"/>
            <dbReference type="Rhea" id="RHEA-COMP:9665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78527"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.21" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000167">
        <property type="protein sequence ID" value="CAD8212174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YM60"/>
    <dbReference type="OrthoDB" id="1906957at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004821">
        <property type="term" value="F:histidine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006427">
        <property type="term" value="P:histidyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000012">
        <property type="entry name" value="Histidine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11476:SF7">
        <property type="entry name" value="HISTIDINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11476">
        <property type="entry name" value="HISTIDYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001549">
        <property type="entry name" value="His-tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="7">
        <location>
            <begin position="134"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8212174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="479" mass="55181" checksum="E047F2B0F8015E41" modified="2022-10-12" version="1">MMTYQHSIKISITFSIKNILVIYNGLKTRIDRGIKNRRIVKRIITIRKTINNKNPKGTRDFLPQQVLIRKQAIKIITDIFEQHGAVEIDTPAFELREILMGKYGEVGGKLIYDLQDQGGEILSLRYDLTRLKRYHVGKVYRRENPSISYGRFREFFQCDFDFAGENDPMVADAELIRMVDQIFTKLDLGPFHIKVSHRKLLDAMINIAGIPKTQFKTVCSSIDKLNRQSWKEVKQELTEQKGVTEEQAEKLSQLVQLKGEPYQMIQQLKEKKLFEQQGQEALDEMELLFNILKQMNGLKNVIFDLSLARGADYYTGLIQETELLGRQVGPISGGGRYDNLVGTFQKNSIPCVGAAIGIDRIFPIIEEKFKNSGKVFRENQSDFIVATIPSHNIDMTAEKFKIYDLLWSNGIKADVCYRLHWNLGKQLTYANEQQIPYAIVIGEDEVKQEIVQLKDLISKKEEIVNLKDLIKVIKQKVEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RW99</accession>
    <name>A0A8S1RW99_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0010232</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RW99"/>
    <dbReference type="OrthoDB" id="293926at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8131680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="128" mass="14765" checksum="1E0B65E274D77416" modified="2022-10-12" version="1">MGSGQCCKSVERIETLEIDNSPPQNIIHSSPFDEAQKMVSLSDSEEEYQPQKHQFGVFKERKKTSQLSTGPQYSFQTIPSEQQFSNFITFQAIPSQQGIHNNVFSKFQKEMNQFAKKETKNQKNLKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RY03</accession>
    <name>A0A8S1RY03_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ethanolaminephosphotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0020105</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8132856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RY03"/>
    <dbReference type="OrthoDB" id="290232at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014472">
        <property type="entry name" value="CHOPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414:SF37">
        <property type="entry name" value="BB IN A BOXCAR, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414">
        <property type="entry name" value="ETHANOLAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="214"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="245"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="280"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="311"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="341"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="390"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="434"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="474"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8132856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="638" mass="73722" checksum="C5313BAA69DD8460" modified="2022-10-12" version="1">MLQKYVKEQYFENLRKFKYNGQNFSILYNWILGDIAQFVVDHLPKTVAPNLITITGFCTLLSSYLLSLYLNPMFDQGLPQWASLYIALTIFIYQTLDNADGKQARRIQQSTALGMLMDHGSDCTAAWITGQLYLSAFKIAFTPFSMLAAIGVGFGGFFFGVYCQQHTGVFQLGVINGVDEGLPVIQLFFLITAIKSSEFWLNEIEILNGINLQYNTILLLITIIACIITIAQFCYPVFKKMNWNIITILNSLHLPIAVVFTFINLIYLSPTNVLSKWFHIYMLTIGLQWSKIINIWQLAIITKENFNQFSFTWLITLGAIILNLYSHYFTSDGFCYFDEVELIFCLLVFSLLSYFHCILSIVTQLCEILDIHAFNKIIAIWQSITFGTKFVLISSILCCILDILNNNLLFNLLVDIPNLTIYQYELWRLLIPQFFHGNIYNLSISLLGFLVCAIEVEKNYGTIPFFCDIFFKNLIIQIIYVLLCFSLSQLTLDVQDTQSFGLWNMTFIYMLNKGLADQDDYQKFLCFPLYLPSKYYPPAFFLIMNSIEYPRLDLIAATLFAFIEYQLFDGFMMKLSKGFVTKIEQSFPFKYIQSRQDFITCDQINHSFNMNDAKIAEYQLEIGMISTSESRVQDQNQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S106</accession>
    <name>A0A8S1S106_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">14-3-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0010639</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S106"/>
    <dbReference type="OrthoDB" id="10010602at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08774">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000308">
        <property type="entry name" value="14-3-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023410">
        <property type="entry name" value="14-3-3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18860">
        <property type="entry name" value="14-3-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00244">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000868">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00305">
        <property type="entry name" value="1433ZETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00101">
        <property type="entry name" value="14_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="14-3-3" evidence="2">
        <location>
            <begin position="6"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="186"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8132494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="243" mass="28407" checksum="4C21020555438480" modified="2022-10-12" version="1">MNKFTREEFLFLARIAQQTERFNDMIEFIKHFLDQELNKEERSILSAAYKNVVGNKRAELRVLTAIEQKESRKQTDQYTLNYIRNYKHKIEAELKNSCAEILNLIDNTLYPNSKQVDSKVFYLKMKGDYNRYLAEFLLDNEYHAAVDQATQAYKDADILAKSNLSTTSPIRLGLSLNQSVFYYEILQNASEAIRIANDAFEQAIAQVDSVNEENYKDCTLIMQLLRDNLTLWNNPEEEVNDDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S4J1</accession>
    <name>A0A8S1S4J1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T0030013</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8133864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="76898at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014821">
        <property type="entry name" value="Ins145_P3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013662">
        <property type="entry name" value="RIH_assoc-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000699">
        <property type="entry name" value="RIH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015925">
        <property type="entry name" value="Ryanodine_IP3_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45816">
        <property type="entry name" value="MIR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45816:SF4">
        <property type="entry name" value="RYR_IP3R HOMOLOGY ASSOCIATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08709">
        <property type="entry name" value="Ins145_P3_rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08454">
        <property type="entry name" value="RIH_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01365">
        <property type="entry name" value="RYDR_ITPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="2325"/>
            <end position="2344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="2356"/>
            <end position="2380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="2460"/>
            <end position="2483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="2495"/>
            <end position="2516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="2528"/>
            <end position="2550"/>
        </location>
    </feature>
    <feature type="domain" description="Inositol 1,4,5-trisphosphate/ryanodine receptor" evidence="13">
        <location>
            <begin position="4"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="RIH" evidence="11">
        <location>
            <begin position="527"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="domain" description="RyR/IP3R Homology associated" evidence="12">
        <location>
            <begin position="2001"/>
            <end position="2102"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="10">
        <location>
            <begin position="2413"/>
            <end position="2556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8133864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2664" mass="314583" checksum="DC5A1DEB8913BA07" modified="2022-10-12" version="1">MNLSQNLRYGSFIRIAGKGISGTKGIITSKGFFDHAVYFQTYEDMDDINNFRDGIFQILPRGSFEIHDEYLKGLDKSPSLQQRMKSEKDQYFNMIDNKMNEEVYFSSEVIFQHVESGQYLACSEECSDSRSDSFGLKLQQFLSSRIIFKMEPSKSYQQTGRPIISKQSVKIQSDVTKFWIDKNMRSPVQLDHKSTQAGLKIQLRALQEDSHRYELIVSHYSDSEWKIQLYDNYEKHQLNKLKLKTNEVVTFKCPKNEAYLGADLQNNAIVLKPTQHNQQIPVECLWELQFHDKPNSELPTITLQPRTLAAIRHSFLGQNNLFVPNANQQYNQKNTTENQQYNNQSKLKKNISKQLRLRHIMTGKLLFCQDLFDSDDCFPAILSDESCTIGLQLANRYEREFMDSSYVNLVRDDNVLTSITTKQSMKTSSEVRIKKSEKIQGTDGFFKQLIQEKDSIKCGFRDRAFSKPFIIQRVKQTFFKLVLQPFSAYDQMLYFVQQLKVNAVNFPYVIPPERMLQIQFLLNRLHEYLMDKIDISDENVDDLPIFERQIILKELYFTEILVELLYHLCYKKGTKKLQVWKKQKENIQTIFSSTYQLLTKLVQNNIVTKLYASQWLEMFLHQYMILNQPAIQSFLSEMLDNNKEAIQKFLNENIIKKLIELITQQSPHDKYLKILNAICISSNQAIRENQKLVLENFFKVAAPNFKFDFKIKQGHIYIQSAEQLTYSRWQNLEQFYKSSLMNDNLEYWNYFISYFDLLGDVAMNRFAQAKEFIDENYSLEIFCKILYEAQSFHMFNLMKPFLKLIRYAYIDTPPFQKFKKLRVVQFKDLQGDFIDHSTMLPKTSPLLDVMGFLLQQLENIDQLHNNLEYSKNLQSVLQLLKCSIEMQFWVDMDQIKVILTAMYNICSSIKNFKMIKDQIESPNIQPSSKSIIHLSNMYQQSEEKETHFHKKIKSTRLNETNNIYMTCKQLACEIINTIFDLENNIRVFMCSKLFKEQLLQLELVDPNLANINNNQKSIKTSIDVQKVPKKNNSIIKVIQQKFKNQNIAEDKSIYDINIWINQFRQIVSDRSLSNFENFELIFAELSFYENLYLPKYSLEILSRAYGQRKELIQNFEKVIIVVSGKTQELSLLAKQIMKKLNILYQSEFYTMYKETSNNIRTSLIWYHSNEHQKAGLMQNLFQLGLYLKKDFDKGRSLNPLNFDDYKPENNTTIAFCEREQNYKLHQAILIAEEIHIPLLEYIYKFSVDQSVIYWKFLHSIYDFLTILIWNNSENKQIIIKNKQLMLSLEQHLKFNVGAIDFLKALYANNKPLLFSEREMTFILHSVAPVCNEQSINCYFKSKTLDFMKALLMFNSNYITSNQTVILQKLQEKQFNKIILQFKRYDDQTPVSPEENSFSDVTSSQDSQIDQETMISDVVFKNWIDQYQKSYENIGYGFHELYISPELTYLYTFFSVFSQLIEEKHLINMKKCLKIHKFNDLIVLLHLAQDCWPLKRHLRAYINRLYYSKVSDHFDLIIKIDLVIMIDDLQKIIDYLILPQHSYMTMTIIKAPIRYKYLMSYIYLNLEEILISLNSLFLNEQFLEYLEELILFNQQHSIYRLHFQLFEICQKLLIIEKIYIKENLGHLCQLLLDVFQSIISGFEVKILLMREGLYISMVEEMLLSQYIKQTNQQDLTKTQVNKLQLIQRNREIYKQMQQNSHDSSVKQYLVHQVFQLDQQFIKESRLNNTLEFWKTQSKQRIKSFKQDMNDLINHKLRRIMNNWSFIPQFSQFLDEEFYEVCNKLSSIGEQSQNAYQTKYEVTTLQDYIQNIISICANTREQQFSDEIRIFFLRLLSRLITEKNLSNKQKMVEVDLWTSEFWIDYKSEINHAQNLLAKCGAQDLILLILSESFLEINLSLLNECLLFSIAFLLGGNTQAQNAILLKLKQDSQNQILTNIKIIITKLTKVINNNFSIVTYNNTKGCDFIQTVDNFDFYNTNTKVMQRLNVVDPDDFKETNFKKQCLDVLCRFFRVMQLLCENNNSEMKEFLRRQTDDQNIARINSINFIEFTSVQLRAYLKILSRAIIIIPASILDFINEVIQLPCIENQITLCHTTFFEDVSNMAHFFANKSNQLQRLFDTEDDLQELQELFNKILQTVLLVLEGNEEKIYKDIQNKLEASFLISFLSLSFEQLNIENINDLEIYLLQQDKIFNSDMLRILNVCIIEQKMASYQQSRWIQQFHEQLSVNPTIKDIYDNLIVLIHHIEIVYEGKSMTVFFPYHPLFNLLSESSKQDLLFQINRETQRDKLLGLLSATNILFYELEHNYKLNHYRIPITQNNLNVMQNLSSILALLINLSMIFFYTVVVKGNTYLLTSTLYNSLIIKMLSVSQLFSQIILFIMVSFQRIPIYLQKNSKGNDKLSLLLVIIQEDTCLLLFLLVLLSFFGAFINSTVFVIHLVEIFSRVTILKNVFQAISYNAKQLLVVAFLGVLFVFAFSVISFSVYFDDIYQEEQTETCDSLITCMITLITSGVIGNSMINWDPLKFFFDMLFTVFFGLLFTNIVQGIMIDTFAELRDQRSKIEEDIKNRCFICAAQRGELENKNQSFEQHIQEKHLIWNYVFYVKCLWLKEWTEYTGLEYWIYEKIKQDDTSWFPESVIDGKNITERLDQLENLLSQVIVYQENNSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S562</accession>
    <name>A0A8S1S562_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0030512</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S562"/>
    <dbReference type="OrthoDB" id="290303at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8134805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="129" mass="15257" checksum="7B5D9B5B3C1580A3" modified="2022-10-12" version="1">MRNNQMRIEFSLPKQDIFSLPQSRKQEQKTLQRRFYRPYRNKSVFPELNSTNALYLSKTSQKFRLSHKEWQDELQTCLQSQSIRGVPNKSKVSLYSSKSAQNFCLSSQLFRRPQADLKPLLEISAQKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S6V0</accession>
    <name>A0A8S1S6V0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">R3H domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0030498</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S6V0"/>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18044">
        <property type="entry name" value="DEXXQc_SMUBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02325">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000326">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050534">
        <property type="entry name" value="Coronavir_polyprotein_1ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001374">
        <property type="entry name" value="R3H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788:SF8">
        <property type="entry name" value="DNA-BINDING PROTEIN SMUBP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01424">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01428">
        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00393">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51061">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="R3H" evidence="9">
        <location>
            <begin position="708"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1052"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1148"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="159"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="319"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1060"/>
            <end position="1076"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8134779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1177" mass="140036" checksum="98523203F34C4E7F" modified="2022-10-12" version="1">MIKQTQKVYESFEHFCGLQQQWIKQEHEEEQKFIQLISQELTHAQIIQMGYGLGKLKLVKTKLTFGNRQVLVFEQQQKLQQEDNKEEQNKEFSEIKQENFRLKQGDQVGIYIWSNVLNQQKFKNPEAIGVIDRYRKNLRIILEQNCKIPDDNSIYGICLQENQVTYKRHLQVIEDLKQQYEKHPIIQILFCDQQEEKIKEKIDQNIEFQGDTIQQLNEQQKIAVQKAMKQPHISLIHGPPGTGKTRTVCEYIKQAVLVQRQKVLACANSNIAVDNMIERIQSISQLKVCRIGNPARMTDQVRYVCIDQLVKKTTSYAIMKTIKTEIQQIEKKLNRAERKEAAELRDKLKQKKKEYFEQQQLAYEEAIQDCQIIFSTNVGSGQFQFQQLTKNIKFDVVVIDECAQSLEISCWIPILKGNKVVLAGDHCQLPPTVKTKNTGLEMTLFERVENELHINTQLTVQYRMNKSIMQWSSQEFYEGHLIADQTVANRTVSDTILLFVDTSCCDFYEQQQESLLNFDDQNKSKYNVGEAGLVQIISEELLNLGVQKQDIGIITPYNAQVQFLKSKINDIEISTVDGFQGREKDCIIISMVRSNQIGEVGFLCESRRMNVAITRAKKFVCLIGDSETVSKDPFLKRLVDYFVENGEQRSAQEYQHDDKIQIPIKIEQVQQDVKKKKNPQPKIQVKEKNKNNKIEDQQIAPNIVQQEQNVVSQLEKQVLDFVNSKESELIFKGQNSFQRKELHELAEKYNLNHESIGNNKLKTLILSKIQNGNQSNLQQNDEEKIEITDNNKSNNQIIDDAKVDKSQKLEKQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQKQQQQQQQQQQKQQQQQQQCQQKQPKQNSQQKQEKKQNTQNKKTKEQSQEEEDEFYNQIVLKDEKKCSFMSNNIQCSQSILWLSQKCQFCNNQFCQYHSYAERHGCEDEACRHAKLQFQSNERKTEKSRTALQEKIQEQQKSRGKKQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S893</accession>
    <name>A0A8S1S893_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0050164</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S893"/>
    <dbReference type="OrthoDB" id="311684at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8136466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="127" mass="15214" checksum="22C40D1326407CF7" modified="2022-10-12" version="1">MKKNCKTKLYSFQITITKKGFLNFKNQYQDQKSKILEQNQSIKYNIQVNDFTKKQKKNNNNQSEQMKKNTICVSKFDNKLLNSLNQLENLLDKSEKEETPLIFAKEEEIKFKYFENNKDDNDKKLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S8S2</accession>
    <name>A0A8S1S8S2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0050259</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S8S2"/>
    <dbReference type="OrthoDB" id="309773at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8136656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="447" mass="52520" checksum="8514AEE9514FC303" modified="2022-10-12" version="1">MSETSLGNKNNNYDQQYYEPELMESSQFQYEKQLEQQQRQNQQLSSFVPPNQYYNGDYLQQEEQKPLNRTSPFVFTAQNMPKINCFNHQGQTFTNFCKCKECILPLCPECVKEHVYEHSQFNSYPKLECLENILTDVHQEVCQQANQLECAKQDITKSQTQAQSYTQETVQKLKEAKQRILQIVEQYFHSLEIELHNRQQKNLDNFNRDGKSLIEILQARLTSHLNFLDKLQKPDCMYSLLPYLLSTTKEDNYQCLQTAQYYYSRFKEASSIITFDSIKSSQLSLQIAQIVNVNHKDLPEFLDIHKLASPEIIQSQALTVKSQNCQVSQINSYQNSKIQESIQVKQQHESLIIQQQPGLEQSYGYQQIQKLPLQQVPNQQIQNPLIYNGNLQQIYGQQQPFKQTQLIQSNKYQGYPQMLQPYQSVQSTYPNLNAQYINQGYTSQRYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SAB4</accession>
    <name>A0A8S1SAB4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0050091</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SAB4"/>
    <dbReference type="OrthoDB" id="308135at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8136320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="214" mass="25462" checksum="9A8E348A825AB348" modified="2022-10-12" version="1">MRTQNHPLIHNPTLPTVSNQEDSFYENNLYYLNRASQERVYQPFTQRINDKTKNQQRNKYLDPENKKMEFFEQLQKHQLYQAKLDKTVQYYSSQKKDSFQQSVNSSKGQFKIASLKVSERNRNLSERTDYIHQQVKEHNIIDDIVQNQDLITKQKGLLEKVQDLKKLKLLKQYKFNHYCLPGVGKSPYVFNDTHSKCTNPGYSRNIEGGKFFTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SCR0</accession>
    <name>A0A8S1SCR0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0060082</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SCR0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8137420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="138" mass="16327" checksum="CFC63483D2B75725" modified="2022-10-12" version="1">MKSQYEKLFPNFKPPSKLDSDIIQKAKLYIEQRQKRMCFTPRKICNQQKCTLLSPKQIINIPSIITVSLSNQLQYQKPQKSYEKTKVSIAHSRKSVPKLNIETMNFRKAKMVFQQRLELRSQNNSKNDVSFFSPWSNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SDS3</accession>
    <name>A0A8S1SDS3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0050369</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SDS3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8136834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="140" mass="16518" checksum="DB70F85ECC85262F" modified="2022-10-12" version="1">MIIEENSLAEELKGVSRYEKVAYISIFIIGLLIIKTDDAYITCQTSNEFQGIIWEMGLIPLAFIGDVTKNLNINIFIVGLVQFLACQLRLQFTMIYLLYKILGQGKMRLIVYFTQYPQQQIQYYLIKKYFLVLHNLYQYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SE75</accession>
    <name>A0A8S1SE75_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0060275</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SE75"/>
    <dbReference type="OrthoDB" id="289984at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="310"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8137757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="377" mass="44144" checksum="339DBFABC95E60E2" modified="2022-10-12" version="1">MYQLSVSQIHFKSLVQTQLMQISFQCADKREPLRIVTTQKIIKYSPSWTSSFDLGRFLQIYIEIQDEESGFIKVIGFAKLELNEIMGKESIYKIDLEQGNQVSIFIKCTNPNEISRNSVQMQSQISWTKSLRTEPIVQKRYGSPQPCSTRVRQEIKKVNLQDLQHVKNIQLPTQSMPNLSESRMSLQKGRESYNSNFTYAGYSVTNKCSQYQQTENADSNIKFKQMIKELSLLLNVPQDCDAIVYAVQKVLQNFRQQSEQLIKIQCDHQILKTEYQTLYHLKNQIETNQEDFKMQIKQKLKNYKTLFEENVQLKKTLKEDLNKIVELNLKIKELKKDDTILTEVNSLNQQIKFINQSVQQSQQDFAILVEEINLIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SRQ6</accession>
    <name>A0A8S1SRQ6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0090462</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8141144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SRQ6"/>
    <dbReference type="OrthoDB" id="293534at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12621">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN CHORD -CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12621:SF7">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8141144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="477" mass="55785" checksum="BD9E9BFA33754216" modified="2022-10-12" version="1">MNKILQSIEQIDIFGVSLQLLTNESKSAFQSKVGGIISLITGSLSLSYFLFIIALWINNSIPPSISLKQQTKGYAEFEWSEPLIQLTLSDFTSEIDPFRKENNIITPFLFNLKNYQIYDEPIPLFSNEFLYSSLLVNNGTLILNNAYQEDEHYKSMKEYLLVLSTCKNSSLLNGGYCADEETINEYISTHHGFIILTIRLNQFNYVTQSLEMFAKQYYQPFDPRRSSYSQVMLKQQETIIDDGVLFKNEKNYIFLNNYEIINQEVDKQFMPDTIAFISEDIYHLDIQNSYLFRIDNISVIEEISMPKLGQVLAQIGSIVQLILMLKYVALYYNNHLLENQLLHDIVSMYYPELKNIKINIFNKFEFKEEINQCQLPIQNLKLIYGQLLKRAKEKCRLNNILYEISRIQFILEQSLGNRILQCSHQLGKKLSKNSNEFVIDRITETNRLVVQPAEQLYQDQDQEHYLNEPLDILTKHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SWN8</accession>
    <name>A0A8S1SWN8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0110359</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SWN8"/>
    <dbReference type="OrthoDB" id="298756at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="129"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8142832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="184" mass="21905" checksum="0462877127F2DB71" modified="2022-10-12" version="1">MRHQNNYIQNLHQVAARLSSKQSNQKSFLNESSYMLTQQSNLSLNQSFNSPKFMSNQQSFLITQPININMSSRNTKQNYKCYSPTIRTKTEKYENDDQKQQQQFIEATIKLLAQSEKLEKLISQQNEILQVERKDKQELEMTIKRLTQEIQFLKETHKKQDFVLENKLKQIKGKSYHLPKKLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SWX5</accession>
    <name>A0A8S1SWX5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0120420</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SWX5"/>
    <dbReference type="OrthoDB" id="288892at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8143819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="169" mass="20463" checksum="192737F5064D6D61" modified="2022-10-12" version="1">MKANRFIIHNPTIESYQLEEYYNHFKRMEDIDKEKMNIYKNIESNKSASNYRFLMLQQTSKFNKQQQQNAQDHLQKLLDNKIERIKVRDNPYVNQTRLRGISQVDLKSPNISFNSSLENLNLMKKLQSLKPNISKKDLDSHYEKQREYGNRLRKLPKLPDSDKKYHYFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SX78</accession>
    <name>A0A8S1SX78_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0120170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SX78"/>
    <dbReference type="OrthoDB" id="270318at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002060">
        <property type="entry name" value="Squ/phyt_synthse"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00494">
        <property type="entry name" value="SQS_PSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8143344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="314" mass="37377" checksum="260066D93D82DAEC" modified="2022-10-12" version="1">MNKLYFVFKFCTDTQKSIYKLENAQKYISELVQKTDWISFAISKHIPKQVRQDYLNICQFHNELARIEETSKERSLGVGKLQYWSDQIDQIFLDNPQREPLSICLHNSCKNNPLPKYLFQKMINYRKQQVEKGEFSNLVDMATAAEANRGSQFLLFLRLMRMDIENQDLKQLTELSAQAVGMTDYLRRVPFTLQNYKLNLPEDIKYKHSINVRSLWDRIRGEPKEELYDAVLEVAAFARTQMIRTSQFQNKIPPQSFRAFLHLTPTEQYLDELEYYNFKIFEKAINQNSYVQLPITLFKTCRKMKYLSKDYESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SZU3</accession>
    <name>A0A8S1SZU3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0130131</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAK10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SZU3"/>
    <dbReference type="OrthoDB" id="286286at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031417">
        <property type="term" value="C:NatC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057983">
        <property type="entry name" value="NAA35-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007244">
        <property type="entry name" value="Naa35_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057982">
        <property type="entry name" value="TPR_NAA35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21373">
        <property type="entry name" value="GLUCOSE REPRESSIBLE PROTEIN MAK10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21373:SF0">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 35, NATC AUXILIARY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04112">
        <property type="entry name" value="Mak10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25789">
        <property type="entry name" value="TPR_NAA35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAA35-like N-terminal" evidence="4">
        <location>
            <begin position="13"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="NAA35-like TPR repeats" evidence="5">
        <location>
            <begin position="306"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8144244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="642" mass="75457" checksum="B85621C6C8A1C6D6" modified="2022-10-12" version="1">MDITAELKSLKNGDILLAEGFSLFEAMSATELFDKKIDVKLGLADADTPQKLLQSQQIKTGSQLTNEELIGLFDLVFQKITCWLNGNALAQTIYTLVYQYDDSLWDNPFVKALFDTLNTVGSHIFKIIKDAPFLREEDYNPSVFSFRNKRISTIQELLTTTLNPLDTELSGKVKKSKQLTIADALSSRIKLLKGFCLIVQCLQQTFNAESQKQITDHIEFIRKRYDTVVQTMAMGKDMSNYQSSTILKHLKSMISMRKIPQMEKPYEYFSTMLNQLEAVLDLSAERNYFNIQQKICTLIHDYPKNILIRAYLEQNLFKYDQFLYFHQTPWINIIIGTLNKMFSQNFDQLYQNVFKEYQDCIVPVTYEYVIKQLRSKFRKSRDFHKNYSHLSILVHHSNQIDQDIFKNKNPLMIFSYEIAVQSMIQQLQYGFDLELYSDVDYASIYFYLEYLTTILDRNRTNYVNQLTNSQREQLQNQNKKKPKVSPLIDQMYFEIQYWKGLNSLFRGLSRLYYTLMENGFIKKVQDMDHRYSNRMKIFDTCYYIKPIKFDEYTQKMKSYGSFQETLKFVSSSLSEAQQLFAQVKDNKFVPQQMQDQSKDLVMICVVTSVNAMKLISNQKYQISFKKTTVSFVPQIELIALQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T2K4</accession>
    <name>A0A8S1T2K4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0150398</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T2K4"/>
    <dbReference type="OrthoDB" id="282432at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8146543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="128" mass="15290" checksum="8F146D54597A493D" modified="2022-10-12" version="1">MGTKRKRVIMQAGSLLNPIFRYVLKMNFVANPTDIHGTKLLRHLQYQTMNLDKKLHQPNFESNYRESIECISSKWEIKFVNGWERTFYMKYADMSTLHQTIKQYNHFIEGQRALQGEDDEPDDNLLQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T2L5</accession>
    <name>A0A8S1T2L5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase M14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0160028</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the peptidase M14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8146740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T2L5"/>
    <dbReference type="OrthoDB" id="10253041at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050821">
        <property type="entry name" value="Cytosolic_carboxypeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756:SF11">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M14" evidence="4">
        <location>
            <begin position="164"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8146740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="561" mass="66126" checksum="F7F6FB7BFD99DAFF" modified="2022-10-12" version="1">MNKKLQLITTHNYSLINYLKVFQKKLQLLDTTIQPQSNQPNGQFTFITTQIQIYSLYDSSNLFYLTEDKDTINILLNSDSNTNGCTQWFHFQVQALKPTVTIKFKILNNRRSQQLLTNSKPYTSKSACFDLHYYKTNIHHPYYANDDSYIAKYQQLMPFYTLEFKFTFQDEEIITFAYAQPYPLSRALTLTDYKTIGYTQLNNPIIQIKRGKAKQYVVILARQHPSETSGSFVVEQLINIINSTKYRYIIYPMVNPDGVFLGNSRCNLHGVDLNRKWINPQLSSEPEVYYIKQSLEKYKQKIIMMVDLHGHSTKQHYFIYGCANNQNNNNEIKNLIAKFQNHSLFNLNYCSLETQANRKSTARQTFWYQYKVQFSITIEISLFGSQNGRFSNSDYKEIAQQIYNSIEKCNEPIADQATLPMIKSLSDEQNESDSECEQDFVHDMVKKKDTYQETKKFLFKQKTIQAETQSIQQDQQLKQLLLPQIKTIKSMTKSEHKSSTKMEFENQMKVKQDSFQKNFNIINYFPQRNTQPKRMSSIKKELNQNKYPQIKIEQLKKYQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T8C3</accession>
    <name>A0A8S1T8C3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0180118</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="286604at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8148543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1467" mass="174128" checksum="9C8027EA064414AC" modified="2022-10-12" version="1">MLNQTLNDAQIVAGIDNLKMKNHCAYNYELYPNGEKPLLLMCTQYLAVDDRLKWNVFLSYVRTLKLKVDRQFYVNLGKIYEQIFSHCYEEDLIYLESEFGKPLEGYQDNQLQELFFMFSKCSPFNNDARTGRFLKIGASLQPENLDDRSTVVAVQLFRKFFLQYADYNFKQLNPFHIIKNNNNMHYDTTVVREKIVNLMMNNQDFLNYIEQHTSLFQSQISFDQQRKIEHQKESLMKFLYENTDLHHFIIMVYDLLNKVTEGEFNSTLILKHISLQILDPFIHPLALSICDFYEQILEEYEGTEDVKQVKDKYLPKITFLNMDGIDFEFNYKLLDNYSDSCQYISVLDEMLDCDDKSQMFDLVTKDKNCFMLAKNSYIIPIRQSHKEKRLNDMHFRHILDLLVYPLIKSFQPQVIIFSYSFAYFNQESDIQLSSKLFTEITTHLSLISNYKVIFLPRILSSGVQIQDHRFEIELLKTDHKNILYYLNIYLTHVQQSFKFLHPSNQQHCELSHYANNAMKIVKSNSFYEKKKQGNIIHYVLNKEKERYMFEILSGFLESCRKSYLQEVKDKDKRMQDPNLQCQDLLQYKLSYAKLLDKLYQYNQKHQFSNLFKSTQQQIHTYKLLFKQHQKTQRREDSQFVSFLAQDLKDYIYVIDNITNNEPQLLQINLRKLQSPVYIHNRLTQYFVNYKLKSILFINVLSFIDIRDIGIDSYQVCLVNYEKYLNPVQKINQIWAEIEVIHETPEPPVFFRSLCFDKLKNIIYCIYGKEAKNNTLCDSIDIFNFEKNTFITIYRDKNTKKESDTYFARAYASSVVFSFEEKQKPYSEVWTFGGSFLGHYNQEYQNMGFCNLVERLFISEESYRSEKINKELIVPLSMRPMYGSLMLSVQDNDESAILIIGGSQNELLVNIDQQEYIGYKFQKIDNQYYVSAITNLGQKINPISFYSQNQNIYSDQDKIYILNDATQILGKSYRFNYETKELEETDPFSPYGILSLTTLKISNNISPQEVFQKQKISIISIDAKNVILNGTQVVQPFAQMHKAMKFLYKEQDNVNYQARIIFQDKDEIVGLIKNFEFTKYIHLYVNIKQDENFHQGYLKPCSMCVSYSNKSLYLLTTNIENGSVYFTIYSYQINQLIQDINLSLNNNNTKLVLNVYTIFAKVVMHLSNNVEKIFKQLTCDDENLYLIGGYMGLIYQQNDLDLLLLQGQQTYKYHKNIENINNNNFKIPFDNPEQYSKFGQSNKYMYESPYIIWTNSEQILIIEKQFKQKYFSEVIDRVHHDFKIWITKFEIQINETWEQGILVLPSFLLSKAYVEMTSQEITDKLKEQIQHRKLDLFLQNTNTEFETIVKIVDYVTIDQRIILTCAVQVLNFIQMLSFEFSLENQEEYLLQYKYNGIHQQHQNARRYSVPIFIPKVTLLVTPQKQAIIDGYQIYDPVNEHQTIRQSNQSQIIVKMKEQIKVYNLIPQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TNP8</accession>
    <name>A0A8S1TNP8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0240118</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TNP8"/>
    <dbReference type="OrthoDB" id="287752at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF83">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="505"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="542"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="588"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="685"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="739"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="778"/>
            <end position="803"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8153267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="865" mass="102688" checksum="93CBD6B6FD4DB729" modified="2022-10-12" version="1">MAEQIDILDFNEAHRNRNSQNQETDRFSARKIKQLLTLEDVDSWVEQINMPHTRNFEFPSSIEKATIHSQSFYIHPNKKFLKENPKIINCDCCGQMIRRKRYNIFTCDMYSIFAGEYGISIPLYFSIIKLHFFILLAIGCIFGIYYCYLSDKYCSQLNQNNHTQKELYEQYCASLLAAQHFLLYNFNQQEIINKNYGEQYTFYILGYIAYLIMMMTPLMHEVLLRLQQIKYWNYDSLNHIRIPKLTLYIKHIPKKFNEQELQLYIQNAIASKYKDKIGGKFIEEIVYIYDIHPIKVLNEKRKETYLKSLQIVNELKINPKLELKGQLIDLVFQLIKQTRDIQKTLQYGLPFTQKVILKFNSESVTNLVYKFYDKSFFNNVLQKIETIKESAQNYQEFQKDKFELQAQLLEQSQTNSSKSSIISQQSDLQEMVRSQSEIFNQPSELKARMKQSIIDLLRLPENDEKQEQTMQQSTIDEKKGLHLQRGFRQDDIFWEHIGMHTLKRFFLRLITTCITLLVGIIIMALFEILFILEQHLKRQVGWVSSLIVIVMTLFTVFLSVFSTILVIFMTKRSQRSTYSLQEKNIITFMSPIQQVCILAFPFIFVLSILQKHSALNSIAVVLAVSKLAQVRLISKGLIHFFHLRSVKFNSIKKKTMKNFMPTSYFQKQLNQLLTPPLFPIRSRLFYILFIYSTGMMMIFFNPIMIIWCLVLQIIFFYYDKYSVLNEYHYDKLFTFQLQKYLILTYQVIMIPIYCYIYVVTFTNTTSHIGSDGSLINEYSTVSSLWTFLGYGTCVLSFTSFLFFRQQIANFFVFVVLRYQKPLRDSTIFNEKSYFQSYNEYFHDLGVHELNSILEDTFKNMQYKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TSA3</accession>
    <name>A0A8S1TSA3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0270200</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="292109at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061863">
        <property type="term" value="F:microtubule plus end polymerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030951">
        <property type="term" value="P:establishment or maintenance of microtubule cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046785">
        <property type="term" value="P:microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007051">
        <property type="term" value="P:spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045110">
        <property type="entry name" value="XMAP215"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048491">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12609">
        <property type="entry name" value="MICROTUBULE ASSOCIATED PROTEIN XMAP215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21041">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TOG" evidence="4">
        <location>
            <begin position="50"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="845"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="845"/>
            <end position="872"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8155605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1593" mass="188013" checksum="66ECED6D5BBFB23F" modified="2022-10-12" version="1">MNNSPIRNQSYYQDKIRYQRSPYSDKENIMSEVNQSQQTFQREAIEILNDVAETSKIDESVLDSNLTLFEKITHHKWQIRRLVLMNLTEEYQKNQTVYEFQSAWLKNIINDSNQFAMTEGLKFIQVFVRSQQMNYHILPYFIGDLIERGSFVKSQIQDLVIEILLQCCKTIQDTTFILSEIHKKIEAKHPKIMTLCLTIIEKILEQYPNHLVKDQIRDFIELCVSLVQHTNKVIRQKSINIFCKLYQMISESFEQVKKTYLDKRLRQIQIKELEQLCSKITKKTQKIDIQSIQPTIQIIDLHTLLPDKFFEMPYYKHNEKKNITEQFAKNLEKYDGDIDNSKDYGSIYAIIITLLEESNYLIYSQGMRCVKGLVRLLRRGIPQPVAKHFFILILDKLKGSLSKPLQSAVFNLLEDILIYENISCDQFMELMINQLESSKNIGLKIACARWFKDGWLALALAVQTGSYESSDSEVLKKFAQSNSEKIQKSLLILYKKVQCILKKENNLNYKKVLLDLINSMQCDEQNLQVTVIESQIAIVNPQLDLQESRITDKQSRKQQQMNSVKSRYQTEEDLCEFAQIGEEMQNFNQANRNRQSIELPTSQLNKEQIEFMNKFSNDLIQLRKLSQNNQDHQIQNKIEEIINSFKIHYSDKNITSNDFLQQMTNNIIIELVHFCKNVQVQKKLIADKLYDFIISLLIKMNKIQVTLDQEMLYQEYKICLIQLNQLDISKLLSLLDSLYICNSLESMIKIGYRIINKYLQKQTNTNIKILQAFINWITQKINAATQQQLDPSFIRTSQKTFENTFKMFPQTQLDIRLLLNALKSRMINQQVEQLDQYQDLLNQSQRKSKSKSFGSQSRFSPYETPNKTQSKLEFSDSEEQESLESLQQIEQQISTQFTLQSLEKFIHYLNRHRAYQLCNQSQHTVFLSPNSSSPGKHWRRGIGRDNMIISSPNRQLYESTKFEDFTKILEIICQNFNFQVQNQVFQVFYLLKHIFPNCQQCLIYLIFAYICPKTEYSENFEVFLSTFLQLDKSIMAYQYRQAFNILYQKQENEKVLINLIHTGFNFLFQKIFENDLLVCIQYFISTLTYMLTQNMFYQLANDLLSKYQQQTNNFNQIVENELINYKKLYDTYLNWLQVQQQTYEKPQIDFQKLYKQQQNDILIVDLQEIDECASKREETVREHINNSQQENFIHINKEDFELLESTAKNFLSQQTLRFNEEGQSQQTDILNIQQFISPLQQISQISNQMHDTVIQDLAKNLYSNDTHRLKELDMIKLQNDTYRQHKRDSNKQVQESYPQVPLKDPMKQKIEKIYEEVQNESVHGNFNEKSLLVDVLAPIETTSLFDQSRNLKRNECIQQMQEILLTSTSLYFDVASTLLLQYENLDMAEDKLAFINDLKLSLVNQTLLTNINQQNFSVIIAIVLKLCTTPATQSEIYNSSLGYDDNQLTKSLQQILDVLLHSKDTEQVLICLIHVFNDHLPMDFTNEISKNEKMMLRLNLKCIERLIQNSEKLHVFGILIEILKFFKCHPPENLIEGLPSIKELELYFRQLKQITDSLLNINFKEGKEFLDFVYRKNNKSIFLDYVKSVIEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TUS9</accession>
    <name>A0A8S1TUS9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA (guanine-N(7)-)-methyltransferase</fullName>
            <ecNumber evidence="6">2.1.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">tRNA (guanine(46)-N(7))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">tRNA(m7G46)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0280090</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)-methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42708"/>
            <dbReference type="Rhea" id="RHEA-COMP:10188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10189"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74480"/>
            <dbReference type="EC" id="2.1.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">tRNA modification; N(7)-methylguanine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.33" evidence="6"/>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8156020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TUS9"/>
    <dbReference type="OrthoDB" id="47276at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043527">
        <property type="term" value="C:tRNA methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008176">
        <property type="term" value="F:tRNA (guanine(46)-N7)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03055">
        <property type="entry name" value="tRNA_methyltr_TrmB_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025763">
        <property type="entry name" value="Trm8_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003358">
        <property type="entry name" value="tRNA_(Gua-N-7)_MeTrfase_Trmb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00091">
        <property type="entry name" value="tRNA (guanosine(46)-N7)-methyltransferase TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417">
        <property type="entry name" value="3-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417:SF16">
        <property type="entry name" value="TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02390">
        <property type="entry name" value="Methyltransf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51625">
        <property type="entry name" value="SAM_MT_TRMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="1 6" id="KW-0820">tRNA-binding</keyword>
    <feature type="active site" evidence="6">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="124"/>
            <end position="125"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="155"/>
            <end position="156"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="251"/>
            <end position="253"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8156020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="272" mass="32001" checksum="0E9C8891E3F7B1D6" modified="2022-10-12" version="1">MNTKPENNKDNNQIEQIEKKVKIQKPKKADFRMHAHVNPLNETLFPYPLNYNQVDWSLHYPKKFGKDPLKLYLNTLEYPITYDDQVNPECSQSIVDFLDIGCGFGGLCFALGDNYPDKYTFGMEIRGKVVNFVGEKIRALRNEGQAHNVSVIRTNTMRHLPQYFRKNSLEKIFICFPDPHFKKKNYRRRIVNPGLLSEYAYLLKSNGRIYCITDVEELHNWHVQHLEKHKMFKRVQDVEQDSTAKLIWNSTEEGQKVERNKGSKFIIVCEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TUY2</accession>
    <name>A0A8S1TUY2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0270025</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TUY2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8155317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="93" mass="11401" checksum="C5F3783CE0B2838E" modified="2022-10-12" version="1">MKLKRLSSIQIQKNHQSLYRKQIFFREIKIFEIRLTVIQITVKNHSVFITKQYSLKLCYKQISLDSNRREQSKFIKLYHVHSKDPLFNFKLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TX29</accession>
    <name>A0A8S1TX29_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0290130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TX29"/>
    <dbReference type="OrthoDB" id="305085at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8156710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="170" mass="20447" checksum="F78325748175CC69" modified="2022-10-12" version="1">MYYPSSTTYTTQSYRKFKKQEDRVENTNAIQDYHLQRTLKQVEQLQRDLEQAKIEEQNSQKQRRLAEEKLYHFQLKYDDMNKECMNLREKIAKADGDRQVIRAQGEGEVKALNVQINYLEKQISELKLENEKLKKENEQLHHQALDAKNQSKRPIQNIPQNTQQRPQSYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TZT9</accession>
    <name>A0A8S1TZT9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0280168</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8156176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TZT9"/>
    <dbReference type="OrthoDB" id="5061070at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1240:FF:000023">
        <property type="entry name" value="Dynamin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001311">
        <property type="entry name" value="Dynamin-like protein-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003130">
        <property type="entry name" value="GED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF233">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_59, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01031">
        <property type="entry name" value="Dynamin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02212">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00302">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="4">
        <location>
            <begin position="27"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="3">
        <location>
            <begin position="531"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8156176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="625" mass="72362" checksum="7C052146349B4702" modified="2022-10-12" version="1">MKSGLWDNMRKMVTLIDDLRDCGVQDYIKLPRICVVGMQSAGKSSLLENICGLDFLPRGDGVVTRRPLELRLVHIQERLSPWAIFKEVPEKKFTDFTKVREEIDRLTDAKAGQRKNILPDPIELTIWSPDCPDLTIIDLPGITLIPLRDSDQPDNIEEITLNMCKRYCEDERTIILCVMPASQDITTQRSLKLARQLDPDGIRTIGVITKIDIMGEGSDASKVLLNEEVKLRLGYIGVKNRSQQDIINKVPVQKALRQEEDYFQNDPRYAGIPQDILGTRALIEKLTTVLYQHIRKCLPDIIKEIDTKSDECEDKLKLLGTELPRDQKDKVQLVFNLLQDYTDGFKNTMDGKYDKNEATITDEEKKIELTGGAKIKQMFNELYQEFADEKYHVTDCYLDKDIELADQLHQGDSIPGFPSFDSFLYLIVPQVQALIRPAQDVLDQIFQYMDEISQQILRKVFTRFPSVLDEISEMSRKVIESQRAKAEQVVINQIEAEMNYIYTNDEEYLTTKADLVFKQNSKTIDPKKILVRELRNRVDLYYKILIRNLRDSIPKYIGYYLVKQTLDKMQLVLYDQMNKSDNVFQMLEEPVYILQERQQLQKTLKALKQAKKVLKQDPDLSKGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U0H6</accession>
    <name>A0A8S1U0H6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Carboxypeptidase</fullName>
            <ecNumber evidence="1">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0300036</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000030">
        <property type="protein sequence ID" value="CAD8157207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U0H6"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000253">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF201">
        <property type="entry name" value="CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036517916" description="Carboxypeptidase" evidence="1">
        <location>
            <begin position="17"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8157207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="473" mass="53807" checksum="D559A588E27D0CFE" modified="2022-10-12" version="1">MNNFVIFLGLLSISFALQVLVDEGDKVDQNELNRLFKINYDGLVYSGYLKANTEGTAQFHYMFYPAPVDALKKPVILWLNGGPGCSSLQGAFNENGPFVFKAGTAEFEMNQYSWTNFANMLYIESPITVGFSYGPQGEQSDESTAKYNINALVDFFSRFTEYKKLPFFISGESYAGIYIPTLANEIIDYNALQAADNRINLQGLAIGNGCTDPTECTDEADPFQIHVYKFYGRHNFISEELYEKILAVQNECYGIKDGKCKELADRVEVEVSGKKEDNIKFNPYNIYGYCFTYTPEGSKMSQKFGGMRSLNEESDVPPCADVQGLYHHLRSAEVRTLLKIRQESAQWAVCSRTLGQYKVNPKGSYYLYQKILKNQIRILKFSGDVDAVVPLTGTMFWIDKLQKELYLATLKPWRPWFVPAKREVDPDQNAGYVMDLDGLTLLTIRNAGHMVPLDKREESEIFMQKFIKGEFFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U1X2</accession>
    <name>A0A8S1U1X2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CHY-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0300259</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000030">
        <property type="protein sequence ID" value="CAD8157617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U1X2"/>
    <dbReference type="OrthoDB" id="296381at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="337"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="35"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8157617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="486" mass="57521" checksum="0FE10F83F5148045" modified="2022-10-12" version="1">MKKQSAKYSKTEPSARRSNKSPTNPNGNPKPEDLLTQSIVNQELLKTQQSFRSSTGPQGQTPSISSKVTQSVTSPVRNSQKRPSQIISKQQLQNMHERSIIKVLGMSMEAVCPKKNHEKNKMLYICQYGDCKADSRLGCSYCLVESHNDHSSQIMEVQTFCKIFDDRKKQFKNMSDTILQVPDKTAQVSQFFEQLRDQIFDRLKQIERNILQSIQEQLAWKPLQKEIINRVEILSSKLVFDMTYEELKESLEFIQGKHFKELEYIKQETNNYLSQKISLVEHIWNDYKIQLETDITNQLNDYNKVMFVDPQSKEFAEYKMQFFQQKQLKVNELSQPFQQQLEMFKEEERKAEEKKQEEIRIQQQIEQRKKEEEIKKEKERQKQIQEQIIKQKESQKNSERQSERDKLLKTFPYKLYTEDCSHRTKCNTIPIFQCCNKAYPCSQCHGFAAHPPRIQVPSYRYCMKCLEIYLVMYPTNQAINCLKCQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U1Z8</accession>
    <name>A0A8S1U1Z8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cation/H+ exchanger transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0330310</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U1Z8"/>
    <dbReference type="OrthoDB" id="196264at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098719">
        <property type="term" value="P:sodium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110:SF187">
        <property type="entry name" value="SODIUM_HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0915">Sodium</keyword>
    <keyword evidence="8" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="97"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="266"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="299"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="341"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="384"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="418"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="10">
        <location>
            <begin position="25"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8159761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="566" mass="64955" checksum="75192F73987AD349" modified="2022-10-12" version="1">MSEYGAVPFALMIVIVIYIVVGEYFKSIHFHLIHETSFGIIIGMLVGLLLKWMDENAYAQFFSLNATIFFYLLLPLIIFSGGYNLKKKQFLKNFKYIVSFGFLSTLINFIITLTLTIAFNSWKLVRLGTNLDSHLPLSYDEMIKYSATICATDSVAALNLIPSQQYPKLFSVVFGEGMVNDAVSIIIFQAVTILQKSGKSFEWYTPFEFIGRFLENCIISLLIGLFVGLFSTWCFKKCRFLTNSTTTETVFAFLMAYMGYSICEMLNLSGVISLLMIGIMMSHYQGYNISQLGRVTTRVTFESLSLGAEAFLYVFLGFAMWNQTTYYSPLTQDLYYVEVSWVFALLQLGVVILARYLSIYIVYWISVAIQGPKVWKLTKYEMTICCFAGMVRGSVAFALIETLVASPTDGIQINQINILQSCVLYIVVVTTLIFGTAMPFFINIQIKKQQEEEERKSISEIPTPVLNDSPKKKELSEAQKIIKYMDEHYFKRWFIYDYENRKERITQEKKQIKTETLQEIYEKAFSEASEDESVKQRNEDFISLPSIEEKPTSPLIVKMQELSQQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U446</accession>
    <name>A0A8S1U446_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0320201</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8158873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U446"/>
    <dbReference type="OrthoDB" id="306711at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="32"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="95"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="281"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="429"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8158873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="582" mass="70119" checksum="5491E47C4C286131" modified="2022-10-12" version="1">MDNRLERNVNKLMDRYKKTSQQIDQIMQYEPTPQQPVTYFIGRSRPSKLNDDDDDSSRERRRQSGSLEQISRLRETLNAKDFQEREKYERIRVLNKELKITLKEYMTVNKELEGKLSAKDKQIRALEQENRQLLDSINKGDANTKEVKIKAETSEKLIEELQQQNHILKQKLGDKKQKLTALRDQLKQAETVYEEQGQNFGRELERVQKLCEDFSNEIKNQEEQNKELEGQLLKQRDELINRDEQIRYFEQMYQDIRYRDADQQSQIEYLNQQVNYFKQQLDQATYKQDEAYAQLAKVEKQQQVFLQSQESEIEKRVSNYKDLLEKKKQKINEQKISIDNLNQKITDFQRLLDNEEKNNQIQLQDYQKLQQINSELKSVIDNQQNKLLTAENRIQSLVLEIQDQKSILQTKKQKTEEFEIQSRQDFRTIEQLKNQVIHLQEQLTKTQQYNRDLENDIQLTKHENDKTMDEIEIKIDHLVKQLAQSKEEIKEYKNRENEFKQKIKSQEEEILKHAQKSAKYKQQIEQSQYSLKQIEEKVRIYESEREIQSRQVLQVKQEQTTTQNKLRVLEDIHSLIKMHKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U7L5</accession>
    <name>A0A8S1U7L5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0350168</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U7L5"/>
    <dbReference type="OrthoDB" id="284635at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23688">
        <property type="entry name" value="mL103"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8160780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="321" mass="39094" checksum="CFC60E1DE452A9C7" modified="2022-10-12" version="1">MQLYLPRFFMSQIFSRFVKPVPPKNVNQKKRALQLAIKEKQQQLEQSGLLDENLKQERFTHTTNYNFETYLKYTKLVKAEREKEIQVIQPKQKRVAIYDRPKFDLSLTDYYVWTSFHDPYYKPDEYSEIVVCKIYLSPKGLIHAFGMGMDPWRNKLATKEFDFEDSNLDKFCLYDAKQTTKYWGPNRSEQFYNKQQYLKPIYRKKRWPTFEEFWSQEEKAPFRVNCTRYGDFRKFKEWIQQEIERCSKLPSFEERVLKKYGQPEFYSDYSKNYQCKTEPAVYKYTREYFLEKGQKLDQSNVLNIPLQPPEHLGEEYRVPNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U9C8</accession>
    <name>A0A8S1U9C8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0370013</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8161771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U9C8"/>
    <dbReference type="OrthoDB" id="412867at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8161771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="197" mass="23630" checksum="9AF5DF4852B79639" modified="2022-10-12" version="1">MELSKQNNEIDKNLQGHTVLFQCLFYINYFKSIRPQNQMLEINGLSCMDQFITLLLTYKQCKIYTISGNDDKSIKVWQVGFNKNISAYKHSLDQYDIYVMPLSLNQSETQLVSCAYNKYEIIILERREWDKFEFKNFVRMDEIYYTNKVISHFIYTQSIQEEGLKINIKENQFNWIASMKKLINSMYLNLKKEKDIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UKA7</accession>
    <name>A0A8S1UKA7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0420132</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UKA7"/>
    <dbReference type="OrthoDB" id="409374at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="50"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="229"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="382"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="474"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="653"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="806"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="928"/>
            <end position="960"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8165280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1035" mass="121347" checksum="9EE01ABBEB1A8BD0" modified="2022-10-12" version="1">MISSSAYTSSFNAYQGIRDFQLFIRPILNYDFCFDENIYPFDGCFAEIYDCVEGCSNCLRGVCLECQGGWQYYEQNKNCLPICGDSIITYFEECDDGNTYQYDGCYQCKYSCQQDCLLCEFGQCLRWKTSYLEFQNQSYSDNLNLKLPIAQKNKNHNQCQYLYDSLECYQYINELTWLFLTCESQYQMNEKRLKQYSKKKCGDLIITCDEECDDGNQVEYDGCYMCKYSCPLNCSKCQFGKCKQCQPRYELINGYCKYNCDGYEIYDDQENRNCINRIINLIENGDQQHYLFNNQNSQYKLVSSLTCNLKDFGIFGYFYNQCRTPVIQYCKESLYDKCLECDEYYKLENNIQICTPLCNDGIVIEREQCDDKNNIQFDGCYKCQQSCLLECLNCVGNKCYQCLDGWQLIDYSCYQYCGDGQITLTIRQKSSTRYTSSYSAYYGIRDFQLFLKTYDNCFDDNIQPFDGCFANIYDCIIGCSNCVRGICLKCQDGWEYYEQNKNCIPICGDSIITYFEECDDGNTYQYDGCYQCKYSCQQDCLLCEFGQCLRWKTSYLEFQNQSYSNNLNLKLPIAQKNKNHNQCQYLYDSLECYQYINELTWLFLTCESQYQMNEKRLKQYSKKKCGDLIITCDEECDDGNQVEYDGCYMCKYSCPLNCSKCQFGKCKQCQPRYELINGYCKYNCDGYEIYDDQENRNCINRIINLIENGDQQHYLFNNQNSQYKLVSSLTCNLKDFGIFGYFYNQCRTPVIQYCKESLYDKCLECDEYYKLENNIQICTPLCNDGIVIEREQCDDKNNIQFDGCYKCQQSCLLECLNCVGNKCYQCLDGWQLIDYSCYQYCGDGQVAKSSMEQCDDGNYNDGDGCYECKFECFPFCKSCADRNTCLVCEKYFELFNNSCKPICGDDYIIIGLEECEDGNDIPYDGCFNCQFQCEKECQICKSGLCIECIEGYMIIKDYCEVNNQTQIMDDEEDTQIQKKCGNGIYSNDEECDDGNLFQNDGCSILCEIEFNWFCNNQLNKSSICTPYTIIQFQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UMQ6</accession>
    <name>A0A8S1UMQ6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0430084</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8165704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UMQ6"/>
    <dbReference type="OrthoDB" id="310600at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021258">
        <property type="entry name" value="Epiplasmin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10992">
        <property type="entry name" value="Epiplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8165704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="270" mass="30707" checksum="E226A772C6B9C146" modified="2022-10-12" version="1">MSNRPPTQQPWQPTTAAPQLQPQWNPTFSPPRAYQAPVQLQSPAYIPQYYTQPVAQSYVAPPVVQQPVVYQQPVITQSIVAQPVMTQPVVQQQPTIKGESRVEYIPYEKSVIEYEEVRQKIQVPREKYVTEYQAIEYQTEYVPQVSYDKVTEYVPVDRYQERVEYYAVERQVVHQPQVQAVAQPIVQQSYVAQPVVTQSYVAQPMVQQSYVTQTVPVVSQPIQYAQYPQYAQPATQTIYGPPVPIGNGPIPASQLPAAVKTGQFQQTPQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UQ59</accession>
    <name>A0A8S1UQ59_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0460082</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8166815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UQ59"/>
    <dbReference type="OrthoDB" id="309168at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="8"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8166815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="120" mass="14457" checksum="A3C4537D121774B0" modified="2022-10-12" version="1">MSDSDESIDSVELEHQMLIDQYKDLLKELNELQQQNQIKPKKQEISMENFEIQWKQLLETKKSLEDKISQIHCQSQVKQEELEDYQDKLLIVKEQISKQNKELQEKEQLVKLLQKQLKDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UVG8</accession>
    <name>A0A8S1UVG8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0490077</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UVG8"/>
    <dbReference type="OrthoDB" id="286161at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8168414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="216" mass="24122" checksum="64921DE80529264B" modified="2022-10-12" version="1">MLTQSILSVESSKVYKPKNSQIGLLSTAISKLGDSNYTTKTRESTNMSPVRMRLFRDSSPKQSKGLQGVVVGEYHALTPAHIVFSSIHKSTSPIRANRNYNESPQRAKVRLDRFKNWDSKLGLELQIQNTKFTRLNNELSYSSYQSHHLSTKSSIFLGNNQSGLGFGGNQSDIRLLSAKLNAIPPTQIGAFSVGHNHELADLQQSLSRILKQSRQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UXY1</accession>
    <name>A0A8S1UXY1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0510138</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000051">
        <property type="protein sequence ID" value="CAD8169571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UXY1"/>
    <dbReference type="OrthoDB" id="297409at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8169571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="579" mass="69417" checksum="4C523A5892D6154A" modified="2022-10-12" version="1">MNKLLYQFQTIGFACDKEYHKGMKEVKRRFEEINQFKRKALKDFNNVDNVINSISDIIEKRKREPLSKSIDCSSFSNNKQQRLQSCLNKMGQIDYDQQQLDKESGIRTASIFSDRIAAILPQKELVDKELNQELAINCHLHDTSKFGQIMEQALLLHSFHKDCSEKLNQNLSLLDPNNFKTRRERAKFQQRNEMNQKSVQAQEYYMKKFQLEHMRLKDKLIDSDQKTLIDHKYCQDRNKGLKSAKNRKRIQQVTEKINTNKILVIQNLFKSGHDQKSQKADEVLRFLELYSKVDRENNQIFSQIININGQFSMKIESYLRKIFFKFSKKECDDLNELYKYINLQGYLECFHLSHLDIEDKELITVYQNNVKIQNLDQDRGLNFNFFRDSLIDIANCISLLPNKEEKLLRLVEEYLLEIYDQVLDLNEIRILTIKSYQEFNNLFFPLIQKLFLLYGQQIKNYNFNDQRLNLDKIILVLMQIKVFPQKFSKQFIRHHYCYSAEQDEGLDLSQFQHFLNLMALSQNKRISISRELNNIIQYNLYKLQSKFEDITEYEQKIFRKIMIFYFHSEFLNLQNVEQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V1Y3</accession>
    <name>A0A8S1V1Y3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0540250</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8171350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V1Y3"/>
    <dbReference type="OrthoDB" id="424834at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052187">
        <property type="entry name" value="MFSD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23512">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23512:SF3">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="357"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="394"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="15"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8171350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="443" mass="50657" checksum="983C5154C09376BA" modified="2022-10-12" version="1">MEKMTSWHDTNLRWFILVLIGVYPLVQFLIGEYPALLGEQIKSYFEVSQAEINYLLSFRSFPNMIMSLIGGLIIDTFGVRRSYVFFGSIVIIGQLLCFMSVILNSFIVMVIGRFIFGLFESSGFVAESYYINKWFKGKENSLAFGIDTGICRLGSIAAAIIYPYLYTTSNNDLSQCLLMCLYIAILSFIIIVILTQIDRCSDLRDKTSDQKLDSIDLRQIKQFSLEFYVTLISCVTCYAVFFIFGYNSVEMFKHIYKLDQKTANTLFSIPYYLSAILSPIVGHYIDKKGKNIEILLIASFCQLLTTTIFYLMPECDTSCVAFPMIGSILNGLFFGTYYAVMWPHIPLIVPSHMVGTGFGLTFACINIEITSFSFIVASMLQIENYQNYQQMNQLMLFLSLVGFIPLVYLFQYHRTQYHKNIHAQTDDNLSTNQIELIVKSKPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V263</accession>
    <name>A0A8S1V263_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0540049</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8170975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V263"/>
    <dbReference type="OrthoDB" id="289076at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8170975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="385" mass="46045" checksum="8E76D62E1FE998DC" modified="2022-10-12" version="1">MKIYKSFAFDQNIPQSDYEKFINPDDLLNESFEELKGEFKNYRTKKGRDIIKQEMEMRKQLQMKRKKNQSGLGVQQKKHEDHDNQKDMYGNIGLLIRQQERKCKQIKKEVQSPNFGQDYTAMKQKIQKTDVTKQPIFSQEDFAKLIQREDEDELIQTQTRTVHKPREGFKSRFIEKQIPLYKNMEQSSEIDSKKQVDSKVLTKPTKIKQFGLQQSRESPSNAMFDGEAKRVSKSYLYKTWLKGEEAQNKKYKKPRRTESYHKAAAQREEIKSQLKERAQVAFKRYESMGKTWEKFGVFSQDILDFRMQTLKEAKIQRLLLEQQEHDLKKVEQNEQRDPDMLTRLVKVITKPNSDNKEEVKTNFVYHEQRLKDMRTRTRQIQRAGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V3T2</accession>
    <name>A0A8S1V3T2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0530081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V3T2"/>
    <dbReference type="OrthoDB" id="294420at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="753"/>
            <end position="775"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8170482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="805" mass="96145" checksum="DA6231ABC3F13C27" modified="2022-10-12" version="1">MLLFLLYITQSLCYLIFEQKGRINNSLHIDMDITKEEKQQLDKDNLWFDFQFDFPTPISIQIEDMITNLDQLLLVQVQDAQYGLFICKDFQLSNQKTTIILDPLPQYQEQWYKYKVQVNKYPRILTRFQSELGFTKNSAEVIYNLNMTYETSGFYQIEINYQYGSGNLELQTCKSNLCYNQTNQTNILLNYSQIECTLIQMDSLQDLCNHQLKVTTINATFYYLTFKHYSSANVQFVKPNKITQIDILNKEASIVLDHLNSLNFIYINQKIKAIQYSENQRKQFDSNIIIFANEVQNLQDWDQNFYIQSSSNQKLKLQYLNQIQILYLNSNFKLEQEEDSYHFYCVDTIFPEFVFELFVYDYELLVQIYFSNVTQYPNEHMNQFELIESNIKSINMNSDTKMYIGIKTKHKSLEYQLYIRDPTFYFLNLFQLRKFSYCKTCQMEFKYASQLKAEIQIHIYYHQMRYEEDNSLFQILLIENGKFIKPIQIYHTQNYLRYSLLTEVGLQYIILTKSNIQQDLQIMITDKKILEIKFDQDICSIDFSVEILKIQQTNNNIYHLYFKEDLEETIKIINRNGQSEVTKIAQNYYKVQFQNNLKEDAYTYFMIEGIKSQSSIILQVQEKPYKNIELISHNIFLSPLKYEQQLKGLTLYIQILDCSQLNCDELNIQKILIITKIGFKNKSIEYTKGYYNLNEYHSTLNDLYDPISNIQTIQLIASINQDNYNFNFTSTEFQLLLKNQIMIQRQEPQLKNLLFAILYIIFIVFLGYIVFKMIFRKKVTIIPKKINKRSNNYEDEEESISIDGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V9S8</accession>
    <name>A0A8S1V9S8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fatty acid hydroxylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0610013</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V9S8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035746732" description="Fatty acid hydroxylase domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8174288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="264" mass="31576" checksum="DE6CACDE957C1B68" modified="2022-10-12" version="1">MIQFHQIFMFLISIMSPVVGSELETLSFTEKHIYINDFKHYVQNIMIGFIGAIAQYLFGRSVIEYYNPMPKTKLRVQNILKEIRYGIPQLMFGSSVTMTFYYYIYPNTPYYKYFEINEYKLLDLFSIIFQWIWITYMSQNFIFSQWLLDSQDATFKIFLLIHPFFLLGTLLLHSSEVLIPIHPLQTMIFGGLYAIRDMLAHDGGKLDYADHYRHHLYYVVNYGHPILDYYFCTSYNPKNYPIKEKCSYVSEFQNKDHPLFSKYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VAL8</accession>
    <name>A0A8S1VAL8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0560017</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000056">
        <property type="protein sequence ID" value="CAD8171966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VAL8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8171966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="68" mass="8113" checksum="F103B72135B72EF2" modified="2022-10-12" version="1">MSYIGFSDHTLLKVLLMFTFVISITSAYVFFYKRQTTYAKNTITDSQILRMRALAQLREQRQIKEKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VBD5</accession>
    <name>A0A8S1VBD5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0600125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000060">
        <property type="protein sequence ID" value="CAD8173901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VBD5"/>
    <dbReference type="OrthoDB" id="297003at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8173901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="386" mass="44753" checksum="81D2A89FABB06064" modified="2022-10-12" version="1">MELKHLNKFVEEYKLIKNDKLIKYLIILGIGFVKQRLEDINQEAIKLLANSFKEKSIHQELKNLRNKVLSLEKHIQPEQQIKYHSQPKLMPPKVTPKITEQIPLQQNVKKAVPFKHHLDDSEIKSILWNQYSENPHHISNNDSQHQPPRKQTIDQQPIIPKYLPRNNSQTEIHPLSQRENSTRQVTQRSSHHSSQFDSLHNRNSPKFKGEKKKSICKENKISGSIGGVPKHLRQVQSKIKSQIQFDKEQYKSNNSQKEEEQTINIANSSLNPFNNTPQNKLFQPCHSTHFGSSQVSSAKIQQQQMQIQDLNNITEKKVFNLDEIASSFLNSPFMKSQITQRLFAKQESASQSSSSSTFSLFNPNNELKNFFQQLDKQVGNSLSFQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VE04</accession>
    <name>A0A8S1VE04_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0600192</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000060">
        <property type="protein sequence ID" value="CAD8174032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VE04"/>
    <dbReference type="OrthoDB" id="303808at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="71"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8174032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="119" mass="14207" checksum="77E370713EA36B2F" modified="2022-10-12" version="1">MNQDQLDNEGDPQQQFLEAFKELLEVQNQRMYNGNDMLNQYCPQLQYPVILPTQIPNTFIINMGPYYPHIQQPQIHPQQTQQARLAPQTQQNQIHNHQSIKEEEHDDKKQAKSWLQQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VGP2</accession>
    <name>A0A8S1VGP2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">2-oxoglutarate dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="3">1.2.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">2-oxoglutarate dehydrogenase complex component E1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0650035</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="6">The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alpha-ketoglutarate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.2" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDO010000065">
        <property type="protein sequence ID" value="CAD8176344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VGP2"/>
    <dbReference type="OrthoDB" id="413077at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004591">
        <property type="term" value="F:oxoglutarate dehydrogenase (succinyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02016">
        <property type="entry name" value="TPP_E1_OGDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12470:FF:000003">
        <property type="entry name" value="2-oxoglutarate dehydrogenase E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11610:FF:000017">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000119">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032106">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011603">
        <property type="entry name" value="2oxoglutarate_DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031717">
        <property type="entry name" value="ODO-1/KGD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00239">
        <property type="entry name" value="2oxo_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006914">
        <property type="entry name" value="PRK09404.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008907">
        <property type="entry name" value="PRK12270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152:SF4">
        <property type="entry name" value="2-OXOADIPATE DEHYDROGENASE COMPLEX COMPONENT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152">
        <property type="entry name" value="2-OXOGLUTARATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16078">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16870">
        <property type="entry name" value="OxoGdeHyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000157">
        <property type="entry name" value="Oxoglu_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="9">
        <location>
            <begin position="614"/>
            <end position="830"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8176344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="984" mass="113520" checksum="DDA211644E076888" modified="2022-10-12" version="1">MLRFVARNLNNVYKFSELQKFGVSFLGSGNAEYLENLFDQWYLDNKSVPATWDSYFRQVLESNNFDFTPEPQNGQSVSVRLDGQSGVRKLLSDHFRVLLLINKYRHRGHEKSQVDPLDLEHIQQIGKVKGYTKLDYSEYFTQEDLDREFYIHAIGSTGITKDKQMMKLRDLINYLEKAYCGKISYEYMHIQSNEERDWFRDQIEKYDEFMPSKEQKLKTFERLGQEHAFSTFLQKKFNTSKRFGIEGCDSMISGLQSLIDAAASAGAEHVVFGMAHRGRLNTLYNVFQKSAEEIMVEFQDLKSTFNEDIWGNSGDVKYHLGSVHNVLFGDKKLRLEMLPNPSHLETVDPCVYGKVRAIQDYFQDSKREKAFGVLIHGDAAVSGQGIVYESLQMADLEGYKSGGIIHVVSNNQIGFTTVPKDSRSGLYCTDIAHAISAPVIHVNADEPELVDKVFKIATEYRYKFKRDIFIDLVGYRRYGHNEQDQPKFTQPIMYEKIDKTPPVFIKYSDKLIAQGIVTKEQVDNLMKTHEDNLELAYQKSRKMDYNLKDWQPVPWEMIKVPVLWGRIKDTGVPLNVLKTLGEKINKIPNEFNAHPQIRKFYEERLSWIQKDQPIDFAAAEAMAFGTLLHEGFNIRLSGEDVQRATFSHRHAVIHDQKDPNGQDYVPLHSVIPKGQEHRLSIYNSHLSEYGVLGFDYGYSITNPNTLVLWEGQFGDFANGAQIIIDNYIASAESKWDVDSGLVMLLPNGMDGQGPEHSSGRVERFLQLSDDDPAVFEKNLGVRLTRQMRNSNMQIVQCTTPANYFHALRRQLRRDFRKPLIAMTSKRLLRLQAAKSKLIELTTQFNQVYDEAFPEFLVEPNQVKRVILCSGQVYYDLIKKREELKQNNVAILRIEQLAPFPYEFLQKIIGKYNKAEFVWVQEEHMNYGPWAFVRPRIQSVLAKTTNISNSPIQYIGRKPSGSPATGFHQLHEKEIQTLLQKAFEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VI41</accession>
    <name>A0A8S1VI41_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA ligase 1</fullName>
            <ecNumber evidence="2">6.5.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">DNA ligase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0610072</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VI41"/>
    <dbReference type="OrthoDB" id="206088at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07900">
        <property type="entry name" value="Adenylation_DNA_ligase_I_Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07969">
        <property type="entry name" value="OBF_DNA_ligase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000062">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.30:FF:000016">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050191">
        <property type="entry name" value="ATP-dep_DNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674:SF4">
        <property type="entry name" value="DNA LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674">
        <property type="entry name" value="DNA LIGASE 1/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00333">
        <property type="entry name" value="DNA_LIGASE_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="12">
        <location>
            <begin position="411"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8174406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="680" mass="78476" checksum="003C63C8D711AE6A" modified="2022-10-12" version="1">MQKNIASYFKSSKKGDDSNDQIKEQQFIEENLKKEKLIVEQKVCSSKSKQHNIEVKGAVLFIDMAELYEQVGEIKGQNSKDAIKELVAKMFIRIMKENKEEFVPAYLFSILKLGPDYESWELGIGQGIIVKSISAVSGKSEKQVRELMNTLGDLGLAIEKSKSTQTQLNKFFVSQTVKEDVKLTMIQVFNTLQQLQKQEGTGSSLEKERILTNLLRMAKPIEAKYIVRFIEKNLKIGAAEKTMQAALARAFQLYHKGIVEGDYESIINQALCECPNYKKIIDTLFSIQSLKEVPELCHIIPGVPCKPMLAKPMKSIQMIFQRFENMKFTCEYKYDGLRGQIHFENGKVSIFSRNLENMTQTYPDIVTYIHTHYKHLDGFIIDSEIVAYDVANNRILPFQTLTSRAKKNVDQNQIEIQVCLYIFDLLYLNKQSYLKETLEKRRQILRETFKEEEGKLKFATSRDSENFEEIEEFLLNSIKMGCEGLMIKTLEINSQYEPAKRSFNWLKLKKDYLDNGLSDTFDLVPIGACYGTGKRKGFYGSYLLACYNEDMDQYETTCKIGTGFSDENLEKFFQFFQEYLIPHPLQEYKCDGVNMDVWFSPVVVWEIKSADIQISPIYSACSTILQLNNKGVGLRFPRLIKVREDKKPNEATSSQFLYDVYKQQAVVASNNTFEQDDDFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VKB3</accession>
    <name>A0A8S1VKB3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0680071</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000068">
        <property type="protein sequence ID" value="CAD8177764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10310089at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="758"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="953"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1051"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1249"/>
            <end position="1271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8177764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1311" mass="154526" checksum="AAE28D80E68CFF1B" modified="2022-10-12" version="1">MQFTYQLGLSAGLFYLSQILVTHSKECNRLNCFCTQNFQFTQEQTWKSPDQVLKQNLNQVLSEILRLYNFSYLKNSQEYLLLLSRIETQYRNDKLFETLLRSQKLSSTKFYGYTVFPYQFRIQGHRQIFYEKLLEKIAGVRINSQIYFNYCNLVDQLGQQIIKLLNQRQQILIFLTLQYQNPLYYLYHNKMYSYIKLVMKCEKLLNDILRLNPNSIVTKNLAYAVYSLVTFQQEKLKQISSLLVLQNFKIDHLWNTLNNMQCCYVQCKYDNGLIMLNHSILFPKLMEENEIQSELVGHYIDRILPSPVKEVHQQFLKRLIEEGKPRLLNNGIQQFYITTNKGFMKEIDSLVRLGQSMEYLTFMTIMGYKQEDNCGKILLSSKGNVYSYSEQAINLLQLNRILKLDHKINLNFKICIPISQNINELCNGFILIPKVNLMQSDQAFKKIQQNNINQVSFYKYFLKHPKQCSVFISIYKFCNSSYDDNKLQYETLYIFQSNEIKELEIKMMALELMFKQVMIVNNIQSETYITSYTSFKSVIPETYQITNSGKSPSYEDEMDDKHFAISNESIHIQQDPLSISLHQFFKQEQIDQKILLNQNNHSSIIANSFQTQDLLISPLRINDEFSNRDSRREHQQLIQNNLNNVDIQTNQYLIIKEDDDIESPIELKVEMEMQVSIKDITDQQMKEQIEWIKSQVRVNKFQYHNKQQHFVPTALKKRSNHKLTLEQQPSTSVKSTESQKGLVNHIINGMIQSNPHQFYSILVVALIYALILIIIVILSIIAINDEILHIFLDSAGGQFSITLLQNINLMEISIHLLANDQKFLAPHFFITNSTDDYITQIRNYNDREFINPEILLNLEGSSICILHTYSNNGNQMKSCFDTRDFLVDQLPLLFHRITEVVEYQSSKFNLSNMEESYFSLNYPIYFDLATNLINQTNVEMSKTKNNSLNIASIYVISGLTSCIGVAFFIFIAEIQIAKWRVSIVRTYFLFQNLNQSLIDQDNETQRLLEIDELSHMKYTLLKLDKNAQQSLKQEQRNIDHRLKKQFWIKQFVLIFLLGALELIYFIPYYELFQSQVQQVIDFHNDIYYLGLAQLAFSNRLVHFIQQYANPFYQVDENKFQLYDELYSYYEDIYLFQLPYDDEHDTYSLTYIVNNDICQLSPIVSMIKLGYTCPYILQQGYSKYLINSKNTLRIMKSDYDITFGFQQILQDQDFIDIIIGYGFVFPQFNLNRDNLFINQNVYNENSKFQAILMAILILIALLMVFLMIIYLISKEFLAIIFAVKHSLLIFSKEQIARNKHLIQVLSKEVTLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VLU7</accession>
    <name>A0A8S1VLU7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0630212</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VLU7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8175706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="173" mass="20513" checksum="40E1C0BEBA09F47E" modified="2022-10-12" version="1">MDNNIWQLSPLLFTKNESKTSSLNRICPNHKKEIILIDMDSQNKKIEDRFACVDCIPENPQIKYCSIETINKQRRDYNSGSYKMLSEYQKKSKYKKTNQFKLIASIRKNCNKKFNEISEKFILEQFQSFDKSKDTKQIKNVTIQALKDEQLLKDLKQLINQLIKKNNLKLIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VQR1</accession>
    <name>A0A8S1VQR1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0710052</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000071">
        <property type="protein sequence ID" value="CAD8179051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VQR1"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000056">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF13">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF19 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 6 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="10">
        <location>
            <begin position="7"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="618"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="361"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="521"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="661"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="709"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="104"/>
            <end position="111"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8179051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="731" mass="84744" checksum="BE564B1C2813D3B2" modified="2022-10-12" version="1">MNPEESNILVAIRCRPLNQKEISNEDLDIVRVEDNLIIILDPIQMEYEAENKKMLEVYHRSKEQRYAFDKVFREESSDDIFEQTCKQLIKPVMNGYNATVFAYGPTGTGKTYTMLGNQETMGISVLTIRDMFEFIKRDLDNDYIVMISYVEIYNEAIRDLLIQQSQYLELRDDPIKGVTIAGVTEYKAISVQQVMNLLLQGNRRRTTEATNANLTSSRSHAVFQINVTQRSKVKNTEMESMNGKLSLIDLAGSERGTVTENRGLRLREGAKINRSLLALANCINALGDKSKKGFFVPYRDSKLTRLLKDSLGGNCRTVMIANISPASSQFEETINTLKYANRAKNIKTKQLPNKKLVAMHIAEYKNIISDLRSEIESLKLRLNEKQFDDNEDESITINNYINKNGKPESEELKSIQEEIFENFQERIQLRRALMEIEEQNAMNFLEIKKRQSEILQWKKSGEKNMPEDIRQGLKSIQTLKTSTEKNMIKKELMGLQLVENINNAKKIRESIPKRIQSKEKRDYLELEIKNHVLELQNVELEINLQIQEKTINDLKMIVENQKKLINDTQDDEKYFEETDIEYIDDLLEDPEFQQEQDDDDLEDLGELDQFKEFIKKEELKQNSPNQFKQRDKSAPRKQFNKQELSIQEDYDEISQSQSKPKVRDQSRNKRDGGGSAIQNKVLPPKDKQLQFQQQIKPNNIKPPKDISVLNLQGRNLSIGNSQQRGNSKAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VR15</accession>
    <name>A0A8S1VR15_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ER membrane protein complex subunit 7 beta-sandwich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0710122</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000071">
        <property type="protein sequence ID" value="CAD8179191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VR15"/>
    <dbReference type="OrthoDB" id="27095at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019008">
        <property type="entry name" value="Beta_sandwich_EMC7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039163">
        <property type="entry name" value="EMC7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13605">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13605:SF4">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09430">
        <property type="entry name" value="EMC7_beta-sandw"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035893530" description="ER membrane protein complex subunit 7 beta-sandwich domain-containing protein" evidence="6">
        <location>
            <begin position="19"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="ER membrane protein complex subunit 7 beta-sandwich" evidence="7">
        <location>
            <begin position="43"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8179191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="192" mass="22877" checksum="34CDD9E27C8E5788" modified="2022-10-12" version="1">MAIFNLMLLVAFPMIVFSAQSKIEGKILQQLQREFVHDDPILKVTLNYGQYETHTQKNGIFAFYNIPNGRYFLEVHSRYFVYESAFIDVYTSNKGTNVAVSKIHPISRQRPKQQQQQNKQQRIQTRDQMLFEIIQRKDYFEKEEELSLSSIYQNQYFFMIAITVLMLFFVKKMPIEELQSAERAPQQQQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VVL4</accession>
    <name>A0A8S1VVL4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0690160</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VVL4"/>
    <dbReference type="OrthoDB" id="299090at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011:SF28">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="3">
        <location>
            <begin position="229"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="683"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="322"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="687"/>
            <end position="705"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8178366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="744" mass="88638" checksum="703B9DFD7FDEB115" modified="2022-10-12" version="1">MKKRTSSAFSEAIHIMSIAKFLQKSTYQHDISHIKDSLTKDPFQRNDQDIQSLYEFIYVYNYVQRLKSKYSDQFVKEICRYLYYKQVSQNTIINTHKTVFLVLSGRVIVTYYDDNDDGEIQNNQKSIQQELTFGMSFNDYGLSENKKCHVRVIRPTEFACLDFKIFNEINSNQRSNDTYEKQKFLKSLSFFSIFSDVEIKYFSYHMEQSIVQKNSFIYQEDDESIEFCYFIGRGEMEIYKQNNQRKQFCLTKLLQGEFFGEDGLFQLNKRQFSAKCSQENTLLYRISLLEFDKRIWKKEQRKCLFELLNQKWIYRFQRFQELTQTNLDKEILSQQIKNLSQKLIQDEQIKIDFEYINNQNQIQTTSTQEQFITASWLLKSANQAQQILQNFKQKTQTKDEIKQITSVSPKHQQQLERIKAKQKNTKNNFSNRQSIIKTNQSPYRMSSFHNKGQGKVTSNMNYLKLFSQETERLNLEQQLTQYLEKLGINNENLQEIQKKSGSATDRYEKDNQFYQTMATYAQFQLYKNLNLQESKSQHTKNLEEEYQQLVQDQQTNIKKAFDQLTTRKSDNMIRKEFFLNKLKKMNKRLKILELIQKGQLECKATQMVNEEGEIVDIDEPIKPDDAYDIVDGKKVRKFNFVTSEIFEQFEKNKYHDFKQQIDQQKQKIQVIKEIVSGKLPISAANDDYQQNQNRTKPSNSFRPSSSVKQVIPFYILQQKQQSNSYKQQQANYQASSLISTRLKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VY79</accession>
    <name>A0A8S1VY79_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0790204</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000079">
        <property type="protein sequence ID" value="CAD8182918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VY79"/>
    <dbReference type="OrthoDB" id="312488at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12395">
        <property type="entry name" value="RRM2_RBM34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034221">
        <property type="entry name" value="RBM34_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF11">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="66"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="173"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="255"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8182918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="347" mass="40573" checksum="0113154893DAD3F7" modified="2022-10-12" version="1">MLSSILTSGKQTKNQINDLFSKQIEIIEKQVESKSKNNVDLEDDNEQGLKQTQEDKEQMDADRIRRTLFVGNVSINAKKNDIRKVFTQYGEIEKVWFRSIPVDRATKKENVKLPVRAAVLMGKIQEGAQSQNCYILFKDAKSAKSATQQDGQLFMNLHLRCTLEGQKKKDHIKTAFVGNLPFDIEEEEVRKAFEEAFGQINYVRVIKDPYRHVGKGFGYVSFNEFQSLKKALQAQTIEIKGREVRIKKAAETTKREGKELNNAKNAFQRIQKKQQFSQKGKRPRKNTQNKEQTQNKQEKQQFRVQIHKKIQKMKKKRYNPKEIKEQINNIKTQRKTQVKDTNIFNQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VZY8</accession>
    <name>A0A8S1VZY8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cation-transporting P-type ATPase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0760108</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000076">
        <property type="protein sequence ID" value="CAD8181379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VZY8"/>
    <dbReference type="OrthoDB" id="158672at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005391">
        <property type="term" value="F:P-type sodium:potassium-exchanging transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006883">
        <property type="term" value="P:intracellular sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050510">
        <property type="entry name" value="Cation_transp_ATPase_P-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294:SF21">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294">
        <property type="entry name" value="SODIUM/POTASSIUM-TRANSPORTING ATPASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="270"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="716"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="746"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="775"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="918"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="947"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="988"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="4">
        <location>
            <begin position="168"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase C-terminal" evidence="5">
        <location>
            <begin position="739"/>
            <end position="1006"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8181379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1016" mass="115607" checksum="8E7F69CCA15A556E" modified="2022-10-12" version="1">MANSEMQDMLICKPRFLEKGPDIQTKLQLSINYAEQDEQMERPVPSVYEKYAEMQEYFCPLDQLEGLLKTNFGVGLSSDEALKRQQSKSIDVSDWKMFIKELNLFAFDLIIATMISISLAIYGSQNNLEFSGEEIITSCLLLIVITITFAMGYQQRKKSQYAIEKDNEQTITVYRDGKMQHIQIQNLVSGDVCVIKDGLVIYCDMRVLESSDLQIQMNQDVTQAVKGSQIFCGSLIRNGFGKAVVIKTGSSTTIAQIQKRQGSKLLQKQLILFQQIMTLIALFVIALVIIWSGVMSDIDTYNVFAYGFRVVEQVIIAIVPVGLLVIVTISFQMAIRRLAKLRFLIKDAESIERLSEINCLCIGLNEVITSKNYSFNSFNDCKQLLYECNKQKSIHLYYCAILTCNHTNLDGTEKSIIDYFKEEDTEDLIKNTKYYVNGIQYHLPFNSSKKYSLSIVEQQGKYFVYIKGAPEVIWSYCNKTLFGKIDLQQTQQFNDQLVHQCSKGLRSIGFAYLQIENEEDLKINENDYEFNKQNFVYLGTLYLNNSLNEQTIPILEKLQKQNIKLVIITGEHPETARVICKNTGYYSNPTIIEGHQLYLHVDDQQEVTRLSSVENKLGNWCSQQEIVFARTSPEQKLSIVKALQQLNYKVAITGEGLTDLGAFRQADISISKKHGSQQMIINSSDLILTSSKQTFLDSYQAILEAKRAVNNVTKSIMLCLSSNTAMILALFVYCFLGTPLPFSQNLMLLTSISVDLILGISLAREELEYRIPNRTNLFLFAILIIGCIEAGGGLMASFSAYRYFGFNLQNLYFTRFARVYPVGDQDTYNPSSYTLGNSNLIDNCQYIGQMQNYQWSSPNNTIDQRLIFVQCGTCSELQIQNGYLTQCWNTKYEYPDCESNINDLQFCYTAKASDFASTSFYIVLVLSQCINYFALRTLIKSYNSTRINTLAIYGLTISLILLPIIVYVPGIQRVFQTINMPWELLGSAGLPFCLLLLATVEIVKWLLRTYVGFQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W1W0</accession>
    <name>A0A8S1W1W0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0800095</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W1W0"/>
    <dbReference type="OrthoDB" id="285664at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8183131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="354" mass="42088" checksum="BD614B02A30D5927" modified="2022-10-12" version="1">MKPLNKNVQVIHDWHDGPVRVNLKKYWKKNPMDYIQLPKPYSQKNKITYSKRIINDFVEVVNNRQEMVNGIVETANHFQMASSKIQDCFAEFYGPETHYQQELDRVLDNVKPEFRYIKAIAHQNLKKIHSLLRKQKHETQQELHPFKIMTKKDKLFLLSDPKNADILKSDSRQSRTETVTITPGEDKFTSQYKIMIQKEHQREQQKQNMIQIRLFDSQLKYQGDDFVKQNIFKSNMKHTYTPKNIDKNYYVNNLRHSTQESVEFKNPSHFKTAIHSRHISKYTSPNGSQDLIIGYDTIKEDKVNPLQYSSEGNKKRSYRSFKDLINQPKEFEIRNMTDTKKYTRTSSQPILSHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W2K1</accession>
    <name>A0A8S1W2K1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DBF4-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0780145</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W2K1"/>
    <dbReference type="OrthoDB" id="286503at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8182339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="233" mass="27036" checksum="8B6FF27EC297C021" modified="2022-10-12" version="1">MDQLSTYQVQLNSVLDLKNVFMMKPRCKDSADTKIAIDYSPMYSEVLGCMLNTVPILNNDGEPSQHGFILQIDQPVHLANSIHYRSQFEKKLQEVQVKNKDLLFPKPRGTKIYCNVCKQYIEDYLQHTESKNHKQKFRKNKVINIIHSMADEFQKNRSMPQNTPTLHSDSDRCGYFSSIQDDSIEHQDSTQQEPHTIKKIKLNEDVNAFNKMGNSLVNSSNQQFNDKYGRQCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W8Y1</accession>
    <name>A0A8S1W8Y1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0850012</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W8Y1"/>
    <dbReference type="OrthoDB" id="289532at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45641:SF19">
        <property type="entry name" value="NEPHROCYSTIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45641">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_6G03870)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="289"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8185293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="416" mass="48362" checksum="42A376DB4B64B91B" modified="2022-10-12" version="1">MYDVEITTKSDDEILSLIRTYQQQGYQVWLQKRCSDKFLMQADYNTQGVLDLDNSGIFKSQNVDPKQFNEPSVICSLRKNVEDGHKTSTMEIKDTMLIFPRKTQTDLYSLLSPKLNSEREDLVKGFYEQLKEALHLEQFGQVKESIALLTNLVNELENQLIDSQILHLIFVESLLTLGILNAQIMKYSDAKMYLEKCLRLFVIKDQILLAQIYRKLGHYSQSVFSYQKSIDYYNQALIIYEIYNLIPEVAYTMMSIAIIYSKVNQFELASKIAYHALEINGSQMNKHTANLYQMLGEVYYNSENYTEALDYFDTSYDMKSTSQDVQSRIRTINFIASSCFHLGEYRKAQELFEVALSQVDDQNSLLEAQLLNNLGITKSALKLDSKEEFEKSLKIFLQFYPQNHPSIKRVLRNDSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W9A0</accession>
    <name>A0A8S1W9A0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0840105</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8184985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W9A0"/>
    <dbReference type="OrthoDB" id="65154at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31652">
        <property type="entry name" value="LIMR FAMILY PROTEIN DDB_G0283707-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31652:SF0">
        <property type="entry name" value="LIMR FAMILY PROTEIN DDB_G0283707-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8184985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="741" mass="87173" checksum="211A49CD6EC0911F" modified="2022-10-12" version="1">MREGQRLNQSFSYCRSSPDKKGSRHNKVISDAYRKFLATYQQLSMLGSQYKYSHNTLMPYIYFFKYMAKRYDETNQKCKLKMWIPDTIILNDVDLSAIWLYSSADGYVYRTDSFTSRNAAQKFTEGSNDPNELIAVIKKPHYKDMELIGNDAKPILQKDVQLLFSNAMSVKQEITCLQKYIKCQGPMAYICRTIWKKNGQTTAWVITNKLTFQDEGEYAQRCLASASKFKQTNIIQCKGGRFIEETIPYIQNLLLYCRQSMNVEFDELGCDFTKGIDNKWYLLNVRGFKLINPSNQIFTRHITHDEQIPVDYERKQISNLNQQWNFTKTQICKFCELIYPAKELTHQLTLKMLIECDLHLLSRNIELKHLQIRNRINFTHLDSAMLFHKYYVCDSCYQLFMACDELIQLEFKMAQKLGIDVYEKNNIISLTQQDYRVKGPNVIQKQANTIYGNGEYFIKPLDWEMLNEVQIKLMPNLPEIDYGTIPKMKRFRIMIYIKTLSQIPIESQFNQCKYYIYYNFLNQEIKFQLDMKKSIDHSKDVLNIDHVKLFYLFAADRQDINCILSQLRIGLLCNQELIGELECELGDFKSSQVTIKEYLKYFSGIQIKQILSWSTDLSIGLVESQQGNINVERIKLQKQGIIYLPNIDYYCSEPLPQEWMNQFEKRKKHNINQIGNISTSFAISDVSNILDHSRVHQTKYPSRKSCTVQTTVPNKLDQSSILYSELLEYQKDTDKILNFDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W9C4</accession>
    <name>A0A8S1W9C4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0840115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8185005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W9C4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="14"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="112"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8185005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="199" mass="24078" checksum="E0406E06C73C58C4" modified="2022-10-12" version="1">MNKIQFSTDYINRINELEVMIKNFEVQDDGLKKEIQRLKQVLFESKESFTLLQKKKQQNKNSYNEEVNYKDKDIIKLRNILNEKENWIDQLNYQIQELQIIQEKYSRVETTVMSLQGEVDVWRQKCKEKNEEASELSQKLIMAEISLQNLKNRQITQIKDVNITKNNNNNYYYYYNRIKSVDTKKRLNRGSHQSQRQQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WBR9</accession>
    <name>A0A8S1WBR9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0860081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000086">
        <property type="protein sequence ID" value="CAD8185945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WBR9"/>
    <dbReference type="OrthoDB" id="10262475at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="336"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="381"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8185945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="463" mass="53105" checksum="CE85F4492FBB0EB5" modified="2022-10-12" version="1">MKNKQTLKDQLSKIKCIEHERQIVQIGLDQNLLPSQRALCEMCGQKAIMVPISIDRAIVMLRMIQNDEDILKDKALVSLIDQCLNGGDRALTVKDIITIGTVLSNKFIKIGDNFVLRSKYIANKDKTQLDTSESFQNKRKLLKKQHQLIGNQSIVIKESTKYEAKTGIEWIKNIIETDQSQSSFISSSFSPIIKIWSGGKFKELKHENQFVRCVTLFDEELIVCYADQSIRIWKQKDGTEWTVDQTLKEQHQGEIWSVITHFETFVTGSYDKTLKVFSKRRNNRLHSNYKSIQTVDINDCQVTCMAIHDDILGIGSRSGSLAICQFTDVWNLTQILSKHTAAIQCVAFKADQNLLATGSEDKSILLWEMKYGDQFELIQEIKDHQSYVFSLAFHPTYAAFLSGGLDNKMILFGLDSKGRWIKYQELNKDNPITVIQFLKDNWKFYIGTSVGTIILHEIEQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WBY9</accession>
    <name>A0A8S1WBY9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fungal lipase-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0860121</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000086">
        <property type="protein sequence ID" value="CAD8186025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WBY9"/>
    <dbReference type="OrthoDB" id="283919at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051218">
        <property type="entry name" value="Sec_MonoDiacylglyc_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45856">
        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45856:SF25">
        <property type="entry name" value="FUNGAL LIPASE-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035933294" description="Fungal lipase-type domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Fungal lipase-type" evidence="2">
        <location>
            <begin position="81"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8186025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="275" mass="30956" checksum="A86B29C9D3E3A682" modified="2022-10-12" version="1">MKFLVVLLALAVAFEYDESLARQLTAFSFGAYCEINDINNWNTGTISEQYPHLTKVQVFENVDMKTRGYIAYNTQTQAITVVFRGSDNIKNFIADIDTKKTNFNTACKCQVHEGFLAAYSSLKIHLNSLIGEYRVKYPYAKYHVTGHSLGGAMATLFASELSMTGIKVTLVTVGAPRVGDSDFYDWFSKLQITHTRLTNKKDIAPHLPPFRFGFEHVNTEVWYYDGVDYIICKEVKGEDQTCSASATRTNLNDHHSYLGWSASSCNSEPVMQISN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WFH6</accession>
    <name>A0A8S1WFH6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0900030</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8187761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WFH6"/>
    <dbReference type="OrthoDB" id="309386at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="19"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8187761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="422" mass="50668" checksum="782F28BA2DA4979A" modified="2022-10-12" version="1">MEFMDRLIYNLKLLKIEQLQWPYKNYSGSEGKIYGCKYNYQNNWKQGILKVIQYDQSLNSKDEQQMYKEIIIYNKLVEDFKNNENLVTIVNIIHFQDLKSFCILMEECDINLKDFKSKLTSLNSKLQFFQQFLKGYKSLYDKRVIHRDIKPENILISFKMGKPIYQLTDFGVAKLCEQFNNTQSQLGTKIYAAPELQYMQSFSNNFQFDYEKISKVDVYSIGLVLYELQFQKLPFDKPKNLKEFHNSPLSSRLQKLGKIEYSDQWFVNLIMYMIQSNPDERVSFQELYKCYDDKLTIFIERCKGLAENFLISYVRRGGDNTQRYNNSLSPNRQNYLIQNFNEFPLQKQQIQNQQFYINKPMPHNPIPPINIQQDIHLSPFKYQGIKLDLNQQKQHQMNYQQYQQCQTDRGNDQNFKNKYFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WGK5</accession>
    <name>A0A8S1WGK5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0900057</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8187815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WGK5"/>
    <dbReference type="OrthoDB" id="303296at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051617">
        <property type="entry name" value="UNC-93-like_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23294">
        <property type="entry name" value="ET TRANSLATION PRODUCT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23294:SF0">
        <property type="entry name" value="UNC93-LIKE PROTEIN MFSD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="103"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="252"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="314"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8187815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="338" mass="39028" checksum="7F059FA640E1B821" modified="2022-10-12" version="1">MTEQTNIGNLIYVCIITVSYSGAIGLCRTVIFSTILKIDDQVINSVSLGIFTLIFLFGPFSLPLYQRIIQKFTYKAVFCITSFVNVISTTLYIIVIEQQPQKIYLIITILIFEAIAAPFLAIFYCAYNYYVRSISNSKNIGIYFGIAYSLFSMQNFIGDIYITFANSMYEYNEYFYYPMLFISLIISILYLFIKEPKFEYKSTSTQSIDLHQMMNQHIYGSEEEQNLQQRNDDPSYSNQFIQIWSIQKKDPLFIYIIPTIISLGIFSAFNVVYSQNMIVPHYNIQLNQQLIAGLTLHGIGQFLGGITVGLISDIYGYLNILIILQIFAIFTYIISDYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WII8</accession>
    <name>A0A8S1WII8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0900160</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8188019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WII8"/>
    <dbReference type="OrthoDB" id="433625at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000052">
        <property type="entry name" value="Aryl-hydrocarbon-interacting protein-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000408">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050754">
        <property type="entry name" value="FKBP4/5/8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46512">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46512:SF9">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0697">Rotamase</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035827317" description="peptidylprolyl isomerase" evidence="5">
        <location>
            <begin position="18"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="6">
        <location>
            <begin position="497"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="761"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="680"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="775"/>
            <end position="819"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8188019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="825" mass="94929" checksum="758BBD2EDB04A64D" modified="2022-10-12" version="1">MYLQIIAILNLLTLSYQFEPVCNKILSKQIHDSLPVNQYILSGQFPVKDNSHSYLAFIFYGSQYASNLQQLNNYNTIVWFNGGPGTSSQLGNYFGLGPINFNEKEKLEKNQYSWNTRFNMLFVDQPIGVGYSYAYTKDEIPNNLDEIASQFNYALKSFIQKCQLQELSKESKWFFAGESYAGKYVPAIVYDLLKQQETIVNVQGVILGNPWTEPISIISEMSSYAFNLGLIDLQERQKLDKILLKTLINIKNQEFSKIADNIQTYLIELTKMNGGMNYYNIKKYGSYAKQNQKLELYLNSETAKNLFKFPLAVTFKLTQNQIEQQGDVYLALKDNVGQNDVINKLEYIISKFPVFIYNGQNDALCTNSGTQRWVNRIKYQETIEFINSNFTTIQFNNRTIGYQKKVGNLGFAIINDAGHQVPRDKPQELFTIINGFIDKLIEKSISRKIDIFELKNLSICSSHQKYLMETEFTNLVEDGGVKKRIIQEGQGDIPIDGSRCKILYKGTLDDGTVFDSSLDKENPYKYTIGKEELIKGFDIALKSMKIGEKVELKVTSNYGYGDEGDTYKNVPKNANLTYEIELINFKQAKKKRWEMTPEEKHQEAINKRTKGTAAFKQQNYKEAQKIYKNALSYCELTTEEGNELKASLQLNLSICCYQLEEYKDSIDYAKKALDLKTNQQQKLKALYRKALANIKIAELEEALADLREAFKIDSTNSAVIEELSRVKQLIKEARIKEKEIYSKLFQQKLYDESEIEPKKIEIKETKSENSSCCDQNEKIEEETQPKTTIENKDVQIQEEKPEVREQSKTENNDNNKIVEESATSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WME7</accession>
    <name>A0A8S1WME7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0950025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000095">
        <property type="protein sequence ID" value="CAD8189887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WME7"/>
    <dbReference type="OrthoDB" id="301262at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8189887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="429" mass="50511" checksum="402BC49EBED1CED0" modified="2022-10-12" version="1">MILSTRILQCFSSEKKILRFIKTCCSPKEIYQIYHKNQEHFTPLLFLEALKHMNTNPVRKRFDLNDDTAFYDELTEVFKQVPMQEKWRFYVQVAKIGRNKLLIKNYGSDLTCIRVKQIAILLWGYHKNGIKKPRLVIHLIDQLQQNLDSLIPVSEKPEELQSLKENQKQENQEDNEINQSDEDENFLEEQLEEDLQSEKRVLRDFRFKFKDLVLIIWAIQDIKLDASALLIYTFKLAINNFNKKDLITNRSLILLLQATINIKDNKQINAYQKLVIQNLSEQDLSNEGILQLLLLLRTLGQLKLSPEFAQKLAYIMLKNQEKSNQPFSSKFASIMIWNLNKLAITDTDIYNRLGLHLTKSNEFKAMDISQAILIYSVQQTKSPQYQQYQFILRSLISKANMIKLDPRSQSIIKESLEQYQPHLIKLLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WN11</accession>
    <name>A0A8S1WN11_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small ribosomal subunit protein uS15 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0280156</name>
        <name evidence="7" type="ORF">PPENT_87.1.T0330153</name>
        <name evidence="8" type="ORF">PPENT_87.1.T0950092</name>
        <name evidence="9" type="ORF">PPENT_87.1.T1080096</name>
        <name evidence="10" type="ORF">PPENT_87.1.T1270073</name>
        <name evidence="11" type="ORF">PPENT_87.1.T1370070</name>
    </gene>
    <organism evidence="8 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8156152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000095">
        <property type="protein sequence ID" value="CAD8190021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000108">
        <property type="protein sequence ID" value="CAD8195043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000127">
        <property type="protein sequence ID" value="CAD8201302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000137">
        <property type="protein sequence ID" value="CAD8204444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WN11"/>
    <dbReference type="OrthoDB" id="298816at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00353">
        <property type="entry name" value="Ribosomal_S15p_S13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.10:FF:000003">
        <property type="entry name" value="40S ribosomal protein S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.130:FF:000001">
        <property type="entry name" value="40S ribosomal protein S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01343_A">
        <property type="entry name" value="Ribosomal_uS15_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_uS15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023029">
        <property type="entry name" value="Ribosomal_uS15_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012606">
        <property type="entry name" value="Ribosomal_uS15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="uS15_NS1_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006331">
        <property type="entry name" value="PRK08561.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11885">
        <property type="entry name" value="RIBOSOMAL PROTEIN S15P/S13E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11885:SF6">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08069">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00312">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01386">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01387">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="S15/NS1 RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00362">
        <property type="entry name" value="RIBOSOMAL_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS15 N-terminal" evidence="5">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8156152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8159454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8190021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8195043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8201302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8204444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="154" mass="17486" checksum="91BF1D06D390F0E8" modified="2022-10-12" version="1">MGRMQAKGKGKGISGSALPYKRKAPKWLTLSSKSIVDQIVNLAKKGLNGSQIGVYLRDQQGIPQTRFLTGQKILRILKKRGCAPKIPEDLYALIKKAVQIRKHLEKNRGDITSKFRLILVESRIHRLSRYYRRTQKLPSNWKYVSKTASALIGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WR49</accession>
    <name>A0A8S1WR49_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0980157</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000098">
        <property type="protein sequence ID" value="CAD8191250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="287672at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000015">
        <property type="entry name" value="Oxysterol binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF30">
        <property type="entry name" value="MATERNAL EMBRYONIC LEUCINE ZIPPER KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="936"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="965"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8191250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1481" mass="173196" checksum="2B9EF3263AE06D3F" modified="2022-10-12" version="1">MYQNETEYYSGSGFKESGIMKKWKEKLLIFYLNTQVLQIQDVKDQTQKQIRIYDYTKKRLGRNQDKYVIKLWNQQNEIKLGWNNEKEYLQWCDCFSGEWQKKLIQRKVLEEFDRSLPEVEPYIDKQPLVIKSQAKPSLLSREKIQKMIPYLPDFIQKDMEILLQNTLQNLDGFSDIQNFKQILDSENVQLYQDTNDNLHMKLIYYSKIEPRRIYDEHIMPSTQNWMPHLIKQFQVYPTKKGCSLVYAELQFDENSCENIKIIENQDLSMNKKDNDIIRIVFTQKGFEKDGIYCIFRKYTGQEIYHDQIDLNLAQHLDQYKIIRSALVVQSSNKQEYKSLLIEEIYVECQDKDAGEKMLKVLLMNITSNDRNLIQIQEQIQQITENVITKNYESLDTLQISIKLTDQEIFRITSEQHQIQSREEFLKLIDKVYNGEYINEAINSYSDQRILQGLTQQLDNTEEDGHFMYTKHYRVDSQRGGLTYHNERLLKDQRSVLLNMIKRIGSNIINGKSVMSVSLPIQIFESRSFLERMARAQGHAPLFLEPAAQSIDVKEQMKLTLAFYFSGFMMGIQQEKPFNPILGETFQGRIKGCPIYLEQTSHHPPISNYIMFGRDYKLYGAFSPIVNMGCNSLSGEQQGHSVVHFLNTNLKFYYINQPFTVYNIITGQRNVNCHKRSFCFQPDLQLCAVIQFNPKDKNSSFFSKSNQPIDYFVGQIHKVTPMCIERCKLAHRTGSGLNMKLKIKQEEILESYEQLTGRWTSHLDINNQRYWDINVHRPFILELESKPLPSDCLYRLDLLLMKRKEIQRAQDMKEKMEVEQRRDQKLRPRRIIFIKILMDFEINDSIQAEKRKYLLLTLNGQPDANIIRFKFFQRQICMADIKIKAENYYQMKIQCLRLFTQQGIEIFQEDLPYIKEGTLLYISDSSDFDAYSQISVYQIVKVLGEGGFGKVMLGKHKVTGEQVAIKLIDSGKLWNAEDIDLVFREAEVMKNLRHNNIIKILNYYTLPNMQVVLIMEFLQGGDLVEYIQEKGGLSEEEARVIFRQIAEAIRYCHDKRLIHRDLKLENVLLTSKVEKMIKIIDFGIATVSTNFTIDKVDRGSLSYMPPEVVGGQATEIRSSIDIWALGVILYALVCANLPFTSRNDDKEYKELVTNMLNPDQSERYSAYQILSHPWMQKVLTQSPTKMLNPRTLANKKNKKTLIQMGIKKEITMAGPSRHSQQPQIRAGATMLKPTSNQGFVQVMYDLQTRNFLKQEDQSSPRSSLNFLGDLVNKSNQEPDEIINSEKPKIKTPLTRKYSDFNQKLIDKIFLEDSKREKIKQWRKSQAKKQGNHLKSQSQGSIINCVQNNYVSARSANTSPLQTASRSGLQSPNLLYSVRSTAKSKRKPQSQLEIQTILNLSQKIEWKQLFASPTYSSRDNPLKIQKTNQHKADQLYKIQIQFKQRKRNSDNLLQKQLNLNNKEITIQKLCNIIFSARVRANNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WTF2</accession>
    <name>A0A8S1WTF2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1000151</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000100">
        <property type="protein sequence ID" value="CAD8192051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WTF2"/>
    <dbReference type="OrthoDB" id="283959at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="366"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8192051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="377" mass="44185" checksum="C51F4DBE50C26689" modified="2022-10-12" version="1">MALIHNQNDVQNMFSKAGKITSEKYIVFQRKEEIINNRPRYFKGKSQSVASSEDVNQQADKKMPEISNKKQQEQITEAELIQRKNQVKLSQPFSKQLSRQVGHYTKLSKDSKPSCGQYHVKYSELDKAIHSIHDYDKSLKHTIPLEVPGKHGEHENIDKPIVDRPRFSRTCFLDLTRQSPRPDIFFGRPTPHPDRFTYLNVTDSWSKIPRTPSVQLEKQLSRDQMIIYKKKQFAPDYHPNFEFGKKQLGSCGAPFDKLEQRKDIMTKIPPYNHETYFEFDVYSKDPNSKLFRTPTAPNFKQMLDREQDGKSLLPSFMQKYSNTRMGITHLHQKMLEVNNFRDGRFQTVTSSFLPSKGFKKRPQLDESSEEIEQMEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WUD4</accession>
    <name>A0A8S1WUD4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peroxisomal membrane protein PMP34</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1010090</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000101">
        <property type="protein sequence ID" value="CAD8192321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WUD4"/>
    <dbReference type="OrthoDB" id="428293at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006862">
        <property type="term" value="P:nucleotide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044712">
        <property type="entry name" value="SLC25A32-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45683">
        <property type="entry name" value="MITOCHONDRIAL NICOTINAMIDE ADENINE DINUCLEOTIDE TRANSPORTER 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="265"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="5">
        <location>
            <begin position="7"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="5">
        <location>
            <begin position="96"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="5">
        <location>
            <begin position="184"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8192321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="294" mass="33866" checksum="5C90BFB37FE1478F" modified="2022-10-12" version="1">MDRLKDIESMAHGHAGALAGLFSTCLLYPLENIKTRMAASQQKEVIQEIIIKVWNEEGLQGFFKGVTPLALGNYISYGVYFFWYEYFKHLFKTDISNSFDLIKPSLASAILTTFVTNPFWVVQSRMTVCKDNLNFFQKTKKIIETEGLEALMKGLQASLILTINPIIQFVIYEAFKRRFQYVENQALINFIGGAISKAISTILTYPYQLLRTKTHIKKNSSKSYFSAAEKILKNEGIQGLFKGLTPKLCQSVLNSAFLLMFYEKIYEIIKQGIILIIIEFLKYRKRIRKLQKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WWP1</accession>
    <name>A0A8S1WWP1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1060114</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WWP1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8194278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="150" mass="18519" checksum="07A6D4EF21486FF9" modified="2022-10-12" version="1">MYGQQKLQDIQKTQMGNDIMDMKREQQLRIKLKYQGHFKKGNQRISISNLQLCDTFLQKIKFEIRYRRSYCNKDKECQYRAFWRPKRTSTRNFEISDFQNQNIIYLLQEKKKAIIISKLQQQKTFLQVSNWYEFNILLLFLENHQKFLFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WY17</accession>
    <name>A0A8S1WY17_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1060131</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WY17"/>
    <dbReference type="OrthoDB" id="306923at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="118"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="205"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="261"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="217"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8194312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="302" mass="35110" checksum="8D990CCCDD54DC6A" modified="2022-10-12" version="1">MQITPKALTALQKLLSINSPIYSHEIRNHEHSDSKSNQKSQQYMSNAQQDVINNRLKQKIPSPSKGIESLLRHSINKASLNVKPQGNVKNELIHVLKQNQNVKENTIKRNGNSLNQLLQPQINQPQKEQSTKLDYKKKRPPQLGDFIQDDDNAKEKNVMTSKSLQRSPNNMNKIASGFFTSTHSKQTHMNVFGSLNEVLRQYIQQKSHKKQNGTARNQDEKNEKKVKDNKIHSVENKRISLSKQQNQNHLQELYLKTSKILNSYQTKEALWKQEKNSLKEEVKFLKQLLQQQQEESNQKYQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WYD6</accession>
    <name>A0A8S1WYD6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1040203</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WYD6"/>
    <dbReference type="OrthoDB" id="291928at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8193647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="159" mass="18791" checksum="7796B024C3A05F85" modified="2022-10-12" version="1">MNKNNNNNALRSQTPFMSENHPLNPYGNNFIDHPYESKIFYKFNSVKQYVHLQEDDQFRISKYSAYFAFGLGGTLIGTIGGFQLLLRYVFKPYYTNAYEHLNQYKHLYLGLLVASSVTFMYTYLTTLYIENVSRPLLYKYLDEAKNNGFQDYEISFKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WYJ1</accession>
    <name>A0A8S1WYJ1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1040005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WYJ1"/>
    <dbReference type="OrthoDB" id="306754at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8193279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="494" mass="59847" checksum="635B7A450DAE3C6F" modified="2022-10-12" version="1">MYQISSFWNNLKNKVQNLVEVQNNSNKDKRIICIQNFLLKELNLNHKGKDHQIEKLVFVRDSQLNIGSIQCQDCFQQQEQSKDYQYSLDLLKILEDVYYFIDQVSTYFQNDQQFQEEQPYHQLKIFKVMKETFDIKSINKDIIWKINTCILEIESRLYFSMENTLEYFGKIHYQNMNRVQMNRFIQNIQNQQKQINTYRSYIKLLPTIISIRKFHPQSEYIYQQTVSYQYLAILINYQIIIYNIKSEVKFSKVNVDYENNKIILFKFTQDEKYLIFAYEKPNHLIIYNMKDKIILNIQDQQPCLHMACQEYINLNYLILYNQNLTLKKCISLEYQTSNNFELINKFSFQEKQIQTFIFYDNQEKKWKSLIRNDYGSIATKKIQITKKFKLQLVQTNRMNRLYYTLISNQKKKIRKVATQDQYQLNTYILSKDETFIISSSLSFYEISTGKITFQIRLNENEELQDVQLEDDKVKILTWDNLYIYDQISQKKTNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WYJ6</accession>
    <name>A0A8S1WYJ6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1050002</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000105">
        <property type="protein sequence ID" value="CAD8193707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WYJ6"/>
    <dbReference type="OrthoDB" id="304447at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8193707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="339" mass="39591" checksum="43BC03270DE785B7" modified="2022-10-12" version="1">MENQINSTNKISYELIAQKQLPQISSCNAIAIETKNSIVVIAEESRIIIYSFKYHSLKKIKQFKINSGNINTLNFFKHRQNFISGCQNSSIYIWPINMIMTSKYIQKLKANSGSTLCLCISKSEDQVVTGHYQNTIKIWSQIEQNSQEWSCLQNLVDHTGAVYGLSMSEYQNKIISCSNDQQVLVYQFSQHYCKWILQQIIQKDGLRLCFITNDIFVFQPYAANYLSIYIFEVRSEKFISTEKVQIQEGGQFCNEFFPCIYNPKKGIIFTKSSYNLNILNCKFQQDFLFKEQQLIEIILNDVIDFGDEYFCGSISDDGEYLFIWNNELKEISIRKYKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X8Y0</accession>
    <name>A0A8S1X8Y0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPENT_87.1.T1150088</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Late endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the amino acid/polyamine transporter 2 family. SLC38A9 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000115">
        <property type="protein sequence ID" value="CAD8197402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X8Y0"/>
    <dbReference type="OrthoDB" id="302047at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF244">
        <property type="entry name" value="NEUTRAL AMINO ACID TRANSPORTER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0458">Lysosome</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="81"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="219"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="304"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="330"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="384"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="16">
        <location>
            <begin position="8"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8197402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="409" mass="46404" checksum="28DF79D15B918F6E" modified="2022-10-12" version="1">MNKSSFIPIFTLSNGMIGSAPIILPVLYNQYGFLNGVVFSFIICLTSCITCLIVKKYHLKKEVDLQFLINRILGPKFQTTFNFITIIFYIACAQIQVIISCSLIYSVISLFVNSAPQNVFTFTQFSYQILGMIYILILLPTFFIKDITPLLKLPTYASLATLLAVIFIIFQGSKTPTISLWKSNDIAVLPSVFQLAFLGHSLVVPLLSKSESKHKSRDIIIAYILTFTIYQVVGLFGSYALFERKPQEGLSGFTILDYFPQSQLFVIILQILLFIQISLLLPIIQYVARLRFFAFFNAKNPKPYMLIIFSFVFAFICLFFEMMNLNLENLISYTGAIIGTIIGYILPIACHIKMKYFNNKKVQELNLIESSQELQTKKIGKLEILFDCLGCGIILSIGLSVLVLRFIYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X9D3</accession>
    <name>A0A8S1X9D3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1160059</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000116">
        <property type="protein sequence ID" value="CAD8197680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X9D3"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="14"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8197680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="356" mass="42501" checksum="6D56779971CE8C6B" modified="2022-10-12" version="1">MYNQKRIKIYIYSYCPDDLIGSGYYIQVFKVKNDVNGDIVAIKVYDHSRVQHQIEKYKFLQEIAALQVLDSLNIFKMHFYSHNTYFTYIVTEYCNQGDLENMIMIRGYISENEAILICYQILTGLKDQISKGVIHRDLKPSNILIQKGIYKIANYGHSRMIYSQNQQVQYNVGTTLYNQTQTLTQNKYSVKNDIWNFGVLFYKILTDNVNNIILGKLPWQADREQEFFQFFTKNPIQFSDNIIISSAAKDLINKCLQLDENKRYNFNQLFEHDLFKCFQIKTPKKIDLNVNQQFSIRKLNKQSKYQLYLRTLVSINLKILKQKQLKRDQIRIHELWRCCLQYTKFTQIEIKDLENY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XB10</accession>
    <name>A0A8S1XB10_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1170116</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000117">
        <property type="protein sequence ID" value="CAD8198125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XB10"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="277"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="580"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="155"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="171"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8198125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="712" mass="82434" checksum="CB905BB9C30DDB54" modified="2022-10-12" version="1">MCIAHKDKNWLLYSNKVFDNLAYKLESTNQSETDQSPEKTGTNNSTKNQLTFLQNLQLDELNNNNQKFVQFDFDDDDLYFDDQYKIKNVNYSKDKNSIQYSKAFSFEIPTDRPKNHVQFQRFFQSFKNPEDESIIKKTFTSDVTMKIEVNNPPQFHLLSSAGKKSPRKKSNVPNKISKQNNYMTPSRKVSADQLLNSKEYRSSGHKQSLLDGEVNLVNKTKPVYVHKMKFLINVLENCRIFDNDDELQIYFIHEVNQILLRAKLKKLESIKKNLLRSISHELLTNLNAVFGYIKQSQDKLLTQESCHIQLEQALSYTKLQLYKIYDFFDYRDILEDSFQLKSDKFDINTVILECVDLFKNQIERKSLLINVELPETSYIISGDRQRLCQVLLNLIGNSIRFTFKGGISIIVQKQEYIESMEGIDVNSANLQDNNLLEVQVNDTGIGMTENELSILRKKLHLTDEDEKVSKHSVGIGLGLSVSKQIIKLMAPKNQNYLSVESTKDEGTRFNFLLKYNEEQNLNSNFLQKSNNHTIPYVDAQSTQQVRVMNYNKCNNLQITEKQGQSELQLDLLINQCHCKIVLIVDDEQFNVDVLSHMVKQLGFDIDFAFNGKQAIEKVQTQLLKRCNQQSCIGYNCILMDINMPMMSGWETVQRIRQIENEIHLCRTIPVVAVTGFCSIKDQQKSINEGFNSVLIKPATKEKLIDTFNRLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XB13</accession>
    <name>A0A8S1XB13_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1180123</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000118">
        <property type="protein sequence ID" value="CAD8198416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="319931at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035786244" evidence="1">
        <location>
            <begin position="17"/>
            <end position="1112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8198416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1112" mass="131355" checksum="46B193DD1D1E282A" modified="2022-10-12" version="1">MGQLLLLLLSCVCVRQQSFLKTLSPRKQYYKIDFNREIFKVNNPQCYYYGIYTRYNPLSDIQQVGDIGILDSNCFHIYSAIDKISNRVNFLYYDCIDYENQQIVKSYTFIGNDGKVVTHSYQLKTQEYESYWYFLGIMVKLIEKKVSVSFFQNLQLLDTQEFDIQYPNYDTDLIFIYGGGLLININQIIFNEQISKLSFFPGSYFLLFPSFASTISQCQESYYYNELKYAQLTCRCKLNNVPNIPDIIISNLIQEFFISEYLNCDSFNFQSWIRIKERNYFIKEIYYQLIKISSNFQNPKLQDDDLCAFQLFYKLTSSINQIIITTYSYIFPTVNIASLENPFLIKTIFDIDLNIQLWHFIRVKKSQNSILVNIYFYEGRKIKEYKFERIVNHFNLIRFKIAYGNILQTLNYLTIQLINMIFLNCEKEINTDQCHLSCLECDGPTKFDCLSCDPTSFRNYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXYSYQIHKNLISNTQIMIRIQSLFMVEIIFNEQISKLSFFPGSYFLLFPSFASTISQCQESYYYNELKYAQLTCRCKLNNVPNIPDIIISNLIQEFFISEYLNCDSFNFQSWIRIKERNYFIKEIYYQLIKISSNFQNPKLQDDDLCAFQLFYKLTSSINQIIITTYSYTFPTVNIASLENPFLIKTIFDIDLNIQLWHFIRVKKSQNSILVNIYFYEGRKIKEYKFERVVNHFNLIRFKIAYGNILQTLNYLTIQLINMIFLNCEKEINTDQCHLSCLECDGPTKFDCLSCDPTSFRNYNEKQKACVCVYGKIELDGECKDYSSFQLSISQEIQKEFKCSHGYFESEGQCWKCPSKIKEKLITCIECITNPKSWINNPYCQYNFVTNNEEIPFTKLDHGVEVYFFDGKDIKLSENQENHSDIYLDYLQTYDHFTYFCQNCQRDLEREQCFIFEYCEQCEIQIFKPQCTKCVMNFALKDGTCTIKKLIQSGKDLICETPYYQTYYNTCILCPIINCLYCFDYLKEDIIKNTIVLTKQIDAIDEQVLVGCAQCFEGYIYDFTIGQCLYQQXIRISKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XFB1</accession>
    <name>A0A8S1XFB1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tryptophan synthase beta chain-like PALP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1220158</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000122">
        <property type="protein sequence ID" value="CAD8199807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XFB1"/>
    <dbReference type="OrthoDB" id="296245at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:threonine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00640">
        <property type="entry name" value="Trp-synth-beta_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000160">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000165">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF6">
        <property type="entry name" value="L-THREONINE DEHYDRATASE CATABOLIC TDCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="4">
        <location>
            <begin position="50"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8199807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="360" mass="40692" checksum="431F9F46E7FE93EC" modified="2022-10-12" version="1">MGCAQSRQNEKSNMISCEIKYLLPELVQAIKLGQQCLYSEESPIKLTPIKEIGGIFCKLESLLPSGSVKMRAMYHMLYRLQQKKRHKGIEQLNLVICSSGNAAMACIECLKLMKQEKKQELQVDQTVSEFLQDDTPTDPGQSALIKDQQLSSKIKPYELLGQLFIFCKHLNELHQFSDLPNVKVINSQLNRSDTEIEAFKYAEDNGYDYLDLYNDVDVFGGYATIGFEIDQWQVLTKTKIDYVFVTLKSGALIAAIAFYLKYIAKNQLKVIGVMLYGSSRREQTICSQIIEGFIDEIMFVTTYEVEKAQCELAKAEEIAEFNSAIAYAGCKKTQYKNSLVVLSGSNVPVSDLSKLISKYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XFV3</accession>
    <name>A0A8S1XFV3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1220181</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000122">
        <property type="protein sequence ID" value="CAD8199853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XFV3"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052394">
        <property type="entry name" value="LRR-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8199853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="462" mass="53433" checksum="7F84E4E14858E896" modified="2022-10-12" version="1">MNKSLGPNLKYLQKIEEQEKMIEFLKIKYKETTGFEAPLLQTQNEVVTQQKINPHSITLPQAKSIIFTQQKEEEKQLEQFQTFYDAVSSLKLPTKLLPQKTRPKNNLKTNIDFKNHYHLIEIIDLSNFGNKKFNRLAFKEFLEQIQDMRTLHTLKLRNNGIDDSYTDELKFIVSNTHIKSLDLSHNELGPKGMEIFLSSIKEVNYFKSFDFSRNNFSHSLITLNQLYSALTQQTDLYHICLDAFKSGQSDYKGADVLIKLVINQKSLRSVIIQDSIIQDLQKLSQSLSSLQCHLTELTLKYCFLTSEHINILAQGLANNQSLIYLNLQHNGLNDLSGKYIAQMIQTNYYLYQIDLEHNQLGNDFIQLISLALKRNNVLNSLKLGYNSISEIKPLLNVIGPENTTLSDLGNIQQNPLLLAQVELLQKRFKGSKDSTSNYIILKPINFTQILHRKQENYRVWNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XHN9</accession>
    <name>A0A8S1XHN9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1240035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000124">
        <property type="protein sequence ID" value="CAD8200268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XHN9"/>
    <dbReference type="OrthoDB" id="308393at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="26"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="193"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="357"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8200268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="432" mass="51617" checksum="E097DE47F9EA6CE8" modified="2022-10-12" version="1">MSSQAKINSLFADQECQNCKKIESEFEILRIDHLKLQEQYQKLLKEKEMIQRDTSQTDNELKKNRQKFDQLQNELTKMNEKYQIIQGELERSNQKIEQLKARNAKNEKEKEELLFSKPVENPEQKQTLQQISILQEQLRKAQHQKEQALSINTQLTLELQQLKSQQKPQQNIVQTVQFQRDSTTNDKVLQMEIQNLRQRINEMNVALQKQQQEYQSLQQESKEQILNLQQQLEQQDKNIDQQQSSTIQLKQLQTQLKKIEAQNKVLQKQLDYKTQRYNEQVDDINKLQEELNNCKTNQIVNFLGDEEKDYKEKYKQVLHQKQELEQNFKEKETFLMQKIQQYEQHIKQKQGNAGLENVKLKEKLDELKELQGKLNQIQEEELTQKRVKELESQLKQCEDKLISSKYKIADMMNLAIEHGMTQLLDKFASLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XJ35</accession>
    <name>A0A8S1XJ35_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1260180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000126">
        <property type="protein sequence ID" value="CAD8201165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XJ35"/>
    <dbReference type="OrthoDB" id="300748at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="315"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8201165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="561" mass="66199" checksum="2AB149EDE7EF45FE" modified="2022-10-12" version="1">MFLPQIIENLDNYQCCYNHNKPLRSVCIEPNYPSNKRFFCEECLKAIHIEVHPLSFIKQKVEDELKNSIQQCEIQLNPFINLLKQFEQKVYNFKDIVLQEINIIIKSTSEWIQKIEVLIKNQFSYSFIDVLEKLSKNIQIKIETQQLINQIKAFNNTQISKLDEKLSKFQQFEQSMAIVEIFQNIKHIQSTPNIFLSQDIQYNFSQDIQQNQKIDQNVLSIQHPYCYAIAINKEKDIIVTSSNQNLRIFQMNQGSIRLMQVHEEKYAKYITTLTFFKSSSLSNNFISGSQDSNIIIWYKNRTNQSQTVWEPQRILHGHTSCINCIISDTFDSLFISGSDDKNIKFWYPQKATKWICQQTVSEHTKSIFGLSINFKGDQLISCGKDLLILVMQNLNKQNWLIKQKIYTQNYGYRLCFITDNIFTFQPHPTNKNEIQIFSIQSNNGNYMKIKDLQIQGDEMSCYYYFPQMYIPQKQMLIAKNGQYVNFIKFSFTQQNTFQDCKLENVIDYGVLSYGEIFGTICEDGEYLITWDLYTKCIQIKKCTESSQTYNNIFRTFTYVSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XJC4</accession>
    <name>A0A8S1XJC4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SANT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1250013</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000125">
        <property type="protein sequence ID" value="CAD8200552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XJC4"/>
    <dbReference type="OrthoDB" id="2193595at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SANT" evidence="2">
        <location>
            <begin position="248"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="210"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8200552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="317" mass="37565" checksum="5D607EB752FA3F9B" modified="2022-10-12" version="1">MKQINYFQQALFIFRQEIMNDMSDEKQQNKYNCTQFEDINGLDLSIRKVIKMDGSAEKATINLQCNSFRVKEQQKQVIQQIKHLARQGSHITGYLELPTQQINQYKKKSNQDALIDEVLLDKFLPPNSITPIIFTKKLPQGGQYRLKGTQPLWVNALESLLSVVVQLYYKSDQKLAEFMKEKVLIDSKKELKQEKKPGRKKKILDNDSPNQEQTEQQQQQQTNTNVYSLLQQDVLDILISPLRNDFPFETWTTKEIAIFECGMCRYGKQYEFLSHLIKTKNAQDIIQFYYFWKFTSHYKLWKINKAYYHRSNLNNYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XKS4</accession>
    <name>A0A8S1XKS4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1290019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000129">
        <property type="protein sequence ID" value="CAD8201775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XKS4"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="123"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8201775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="490" mass="57589" checksum="08F8A18FE475EB44" modified="2022-10-12" version="1">MRQKSRTVHEGVQNTLQSVDYNECIYQCILTKKKFIGKGPRYIYLFKNHICIGKSPKVQIPERQFVINTDTRIQWQYSKNMLKSITFQVGSHSYEYFGSNEQLREIKQKFALYAFQMKIQDEYQAESVLGKGSYATVLELTSLYNHQQYASKCIDQQKINEKKNGYRQLMQEIETMRILSEHKHQNILQLHELYIGNQNYYLVMELAKGGSLLSLMKKRSTLFSRNDIRIIMRQLLEGLEFIHSFNIMHRDLKPENILFMSKDLESLKIADFGLAQSCDQHPYTYPKCGTPGFVAPEILEQDSEFARYSTVCDIFSAGVILYVLLVGEPLFEKKDRREQLELNRRCEINFQRFSQDQLDDVERDLMSKMLAKNPNHRWKATELLKHKFFIQSEDQIENEIDYPKQVNMAILKKSAMPTFSKNPFMQQLNRDKALTTRDQSQTLITRPKNCTLIMQTNLNFDKSSQEQIIPLEKLTNFIGSFNIIKDEINQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XTY9</accession>
    <name>A0A8S1XTY9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1380008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000138">
        <property type="protein sequence ID" value="CAD8204620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XTY9"/>
    <dbReference type="OrthoDB" id="303313at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025533">
        <property type="entry name" value="DUF4419"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31252">
        <property type="entry name" value="DUF4419 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31252:SF11">
        <property type="entry name" value="DUF4419 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14388">
        <property type="entry name" value="DUF4419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="194"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8204620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="276" mass="32591" checksum="363C949D571DD066" modified="2022-10-12" version="1">MDTMQKYFKYVVGAGCGISKIKLQGTLEDWQQLRQNVEPLREFELDWWINEIVPILDQFINLYQGNVDKVFWNNIYRFQHPDPKIYDIRPGFATGWITYFFPYRVNENASINDKEPFVKNEFSKTWDKFQHIYPEQFPSGVSKAPVTLEWRRQEIPIQFSSGYFGITLVDNEFLKPQLGWAIMQLNQSKEKQTKNTQKQQLLPKQKIEKPLQIQQKSQPTKQIQKPIQDQKQQIVQSPQIKQNTIKPAPKVQSTKSNQVTVKSNQPKEQQNKISKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XVD1</accession>
    <name>A0A8S1XVD1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1370122</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000137">
        <property type="protein sequence ID" value="CAD8204522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XVD1"/>
    <dbReference type="OrthoDB" id="540004at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861:SF1">
        <property type="entry name" value="TRANS-ACONITATE 2-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861">
        <property type="entry name" value="TRANS-ACONITATE 2-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="3">
        <location>
            <begin position="52"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8204522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="234" mass="27340" checksum="91623D9A3D90DC8B" modified="2022-10-12" version="1">MASFIQTLLTKILHILYVIPSTIYDMIISNFLTKYSYERLVFYMNQKNPKRILDIGVGTGSPLKHIWNQLPQNCEVLGVDIDQAYVLKANNIFRNYNLGGRKLEIRYQNFYEVSEKKEGKFDAVVFSSSFMLMPKRIEALELAKSLLNPGGSIYFILTLQPDSKKNSTFLQFVEYIKPKIKYFTTIDFGQITYEKEFETLLSKTQLKIVNKEKLNKDFNIPTKIFRVFVYETKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XW59</accession>
    <name>A0A8S1XW59_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1380062</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000138">
        <property type="protein sequence ID" value="CAD8204728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XW59"/>
    <dbReference type="OrthoDB" id="310533at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="1">
        <location>
            <begin position="165"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8204728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="940" mass="112912" checksum="D7E215768AD9F7AA" modified="2022-10-12" version="1">MKKYEVVVNNGLFRFEYEPKSHSANINDVIQFINQQKGFPAKSQLYIIVDNNIKDTKFDLKDAKTNIINIKFSDIIETFTIQHINKDEKYKLVLEKVDTCQQLIEIEKKIANLIKCEEYPIKILKKNQFFDRNLLLCQIFIYQQEIYYHVLAMFIIQYKDKRQLFQIDAFSHFDKIKQKIRQKFGIDEEIELYFRGRLLEEQQTYQFYKIHQNAELELRVKSMQKIQILYQEKIYEFNLSVNLTIEDIIKYNKQFDQIHQNQVLQIHYNNQCLENNIRIGDLDLSNDILFIMTNKIQEKFLLIKFVNKEQQQQNYQFRVSNLEYFQIIYDKYPFKGKIFEFYVNDIKLAINDKLMFNDIQFKDDNNYLIQYQLILNYQIINVTFQDEEGNVLKIQVKSEDIIYDEIMKNLNNENARIKAYKKGQLIDLNGTYKSEEIQDGDIINYYFAEITIQFMIWFRDQHQIQEINVNYLDRIKDLKEKIINQHKITDECKILLDGDILNDDVCLKNFKQNTIFRISKIFHFKIELLLDNNIEQSECRFYETDKISQIKYQYREIFWDEVCIQVDNKEDLDDNDELSDYINKVLRISKKNINVRYKFDGTQIVYQDKYSKLITLGEMLKIIANRQSRCYICNNLMDENIKLDDLNLDPNTEITIQCTSRAQFIEFNNNLNKFDIDFYPEDIIGQAIKNHIKGKFIIQQDNKELNLQKSFQSENIQNGAELIIIFLVTIKFKDFETKELLFSQTYKGNMKISQILNQKNFNKARVYYLEKEINQDKTLAELGVIDYSELNIEVLENILILHLDDKITKIIINSNITIQTLKQQQNLNNENYQIYAIHDQTQVLSNDIILKTLLDENNEIELIYKMNNSYIQIHLIDNEDRQYTENVQLNQQVKTFINELKIKYNLDENPQIFYEGEILDQEQYFSELQITQDTLFEIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XWG6</accession>
    <name>A0A8S1XWG6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Insulin-like growth factor binding protein, N-terminal</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1380095</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000138">
        <property type="protein sequence ID" value="CAD8204792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XWG6"/>
    <dbReference type="OrthoDB" id="300641at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8204792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="786" mass="89564" checksum="B7493D71FBE4290E" modified="2022-10-12" version="1">MEKNYQRNKIKTNQFCKKKNHEKILFENKMLAANKIVHLNLKTILMDSVKTFLQFFESFEQFTSQTSILQYSLIYDPLKIDTSPSLLYNQNDYLFGIFKFNSGFCRFISFQTSYYKNYLIGLRIELLIFNSIPLGCGMEFKINNTYYGSIFQTTQGVQTHKIKIEDTMSPSSCYSTDSVYQRFSLNGFFDIPQYSFLLTAQGNYTNAEAGWGISSILVTNGYCSSTFLICDAWNQSTCVSSCSQPYQNLVNSLCQDNDDETPYSKYLIKEYINLAYDPTQYSKYVLLSQNCINFLKGSSIYYSYWKSTRVFGGQYVWAQAKFSRTFNQLNPHHSVTIGFYILYGPNLPADGQFIYSVDSIIQQQLYYQYRQYKISENSGEITITYNSNVIKFSQAECLSNQYYFKPTLSCLDCSSSCLSCTSQLHCLTCQTSYIQTKEGCVCKINQYEYQNQCYDCPIECNQCLSATYCIECLIINNRRLLNGQCVCIDGYYPIIDNQICLICHKFCETCSGPTNNDCMTCNNISMIEKIGSTCSCPLGTSYQESTNSSSINGFLTCNSLKFRVLKGLECVCHPGYYEVNNDCIACPIELDSTLSQCYKQCNNNSQIWHSQNCLQCDSGFIIEFDECRPICGDLQVLGYEQCEDGNSLFDDKCYNCQFQCPQNCLNCDSSTVLPCPDICGDGIITGNEECEDGNSIEYDGCYNCRYQCQPECTKCIKGLCQECATRGWQLDPTQTPWVCKEKCGYGIKVGSEQCDDANSIDTDGCKDFPFFVDQVAYLVITIQILA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XXA7</accession>
    <name>A0A8S1XXA7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1430018</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XXA7"/>
    <dbReference type="OrthoDB" id="286709at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8206076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="249" mass="28871" checksum="58BEC75DC617C73E" modified="2022-10-12" version="1">MLQIFLVINILFARRVNELGQLLTRDQLPVGVILTISYDKLQVVCNEQGYFLLKQEKFEVDVLGLFKQTFYPSPLQNTFIIDNNKTTILQISGFVNQSANLTLNVNQPLNLKQREYEIKGQFTIKIDIMKLLVYSAKVLVSADSFKNNSQVIYLYGSYQYDLGIIELIPVEIDVKLKFNFNFDTECYLMCTKYNGKLYVLQNHTQEETIEFNGKLFDEWPQQCLLYSDIGQAKLLELDGPISDLTRHHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y036</accession>
    <name>A0A8S1Y036_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1420110</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000142">
        <property type="protein sequence ID" value="CAD8205998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y036"/>
    <dbReference type="OrthoDB" id="1668230at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF13">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="130"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8205998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="375" mass="44512" checksum="A08D756A27817170" modified="2022-10-12" version="1">MGNGQCQQTNQKSEVNKIITEENVHSDESDHNLTSKITDRVYKVNFARNELIWRLQSINQETQNFLNITKNSLLYVTELILSLNKYTIQQLKTMIIGAHKTYQILIEAIIKMKPENNEIVPVLSEEYNNFKLFYELQAQPAQQLYYRPVYNEDFEIIREIRVKRIRNYVIKEDNITDIQKTQQRLQYWIQLSQKNHPNLLNLRAAVIQKNICKYVTDYIDGITLDKFQNKYIQNQDKIKIMQQMIDALKMLHSQQLVHGDIKPSNFMVTLQQQIILLDLDQLGAERQSADMTYLYSFKNEQCPTFQGDVWSLGLCIYSLFFGQRIEKNWTIEDYKRRDFPAFNIRSYEPIQQILDACIIDSPKDIWYISTLSKQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y231</accession>
    <name>A0A8S1Y231_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1440003</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000144">
        <property type="protein sequence ID" value="CAD8206382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y231"/>
    <dbReference type="OrthoDB" id="303226at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01610">
        <property type="entry name" value="PAP2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="318"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="364"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="390"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="2">
        <location>
            <begin position="79"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8206382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="460" mass="53629" checksum="4F8C19760061EBE7" modified="2022-10-12" version="1">MNKIILSILYTSLLIAYIVIDIAFNDELWDVSKSFALSMQKSIPVFEQLVYEFFSDIVFIPPIAAFYCFIYDDNKLNSLLYLSVVVTSVTTNEILKNIYHQARPYMIEPEIKSLKCNSDYGKPSGHSQNSLVMLILMPVLLFPSIKQLRRNKVQVEFQENLDKGESHSGYTIYSIKTSELRVTIIRIFSQEQLIISKYEYLIQMQNCFEIDAYYIHPSQYSDDWNFQNISWSSFFRISDSWMGLGYILKLLNPQRSRLEEINHFIPMSFFLLLILLGLSLGLLKLNSLVYYDKQDMSKWIARINECQSTHYTKDSPQILFNSCFQLTAIGAVITGLLVGAMLAQGSYEEGLFNKWRQKLPKMQHFKRLLLLLLPFLFLIPFLFIKSNSVVIQLICKILPLSFGAGFIINGVFPYLLRRFNLQIPGDFLFLCQRKYLLGDQSQNSLYQQIKEYNDQYQHTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y4C6</accession>
    <name>A0A8S1Y4C6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1520034</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000152">
        <property type="protein sequence ID" value="CAD8208789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y4C6"/>
    <dbReference type="OrthoDB" id="286156at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8208789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="95" mass="10703" checksum="69F19E5BA55CE60B" modified="2022-10-12" version="1">MQKQQALAVVRAPSGITKQPFKSINTVNQAPLQRVNSAGFVSGNRQSNQYQKPATPFYYTNNYRLDWKNYLRSLPSVPKIKILAPGTGYDAKMIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8X8</accession>
    <name>A0A8S1Y8X8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1490074</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000149">
        <property type="protein sequence ID" value="CAD8207984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y8X8"/>
    <dbReference type="OrthoDB" id="304528at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8207984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="210" mass="25267" checksum="2A984628BDDC0D66" modified="2022-10-12" version="1">MELLNNQEKELLSKYKKYISNTKTQEPTKYSDIFIGKHQIDDVQLKDDIKFQNTKMSVKSSSDNQRNEFKNIMSQLRNSQIIKRENENRNQNSMLFEEISKQKQQNDQSQMFLIKECLDKPEKQVRQISSISQDYLEQKNIKEKIKSRQSTQDIQKQFNEKLLQQLERLIQKKKIDSKFLFCMIQKHIEECPQFGKRVKEDIKELLQQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8Z3</accession>
    <name>A0A8S1Y8Z3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1540086</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000154">
        <property type="protein sequence ID" value="CAD8209367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y8Z3"/>
    <dbReference type="OrthoDB" id="10047021at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase" evidence="1">
        <location>
            <begin position="251"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8209367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="458" mass="53305" checksum="439563E0DB5B2341" modified="2022-10-12" version="1">MINRLLRLCFCSSIKIEPVNIHYQDGLFDTTYGLLKMTRNPYLNQKQLESITNAYLLVDGVGSNFIKSLFKKDMNSLIIFDCEKMHLRNAIEIYQATKKQIDLQFFGTPKRPRNPFSKCKGPKDIFNLYRHIQNQQKLMLSRMCLVVSYNGEVRIQGTLPECGDLKYHLEEQPENISHIGYRKYNYLLPFSAYQGIVTAYNYEKEGVAIEFLNYKKIYALYGVFPPSQRQYQVLFHSYMQKLVKIHDVTHSSLYNVIDLGCGTGVLGFIANDVLLRSLKDKEINIYSLDNVENAVKSAKINAQCLEYKNYTAELGDITDIETLQYQLTVFKYPAKFNLIIANPPWIQASKIRIDDSIENTVTDPEGIMLKSVFEFANRYLQIESLDSTKGRLILIYSDLSQLLELQEKEKVQELCREYKMAITYYSELPFHIKDPQNVDPLLQYKQNSKVQLFEIRKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y931</accession>
    <name>A0A8S1Y931_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">60S ribosomal protein L7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1580009</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000158">
        <property type="protein sequence ID" value="CAD8210111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y931"/>
    <dbReference type="OrthoDB" id="28644at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01657">
        <property type="entry name" value="Ribosomal_L7_archeal_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.20:FF:000003">
        <property type="entry name" value="60S ribosomal protein L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039699">
        <property type="entry name" value="Ribosomal_uL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005998">
        <property type="entry name" value="Ribosomal_uL30_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035808">
        <property type="entry name" value="Ribosomal_uL30_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_uL30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012988">
        <property type="entry name" value="Ribosomal_uL30_N_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01310">
        <property type="entry name" value="uL30_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08079">
        <property type="entry name" value="Ribosomal_L30_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL30 N-terminal eukaryotes" evidence="7">
        <location>
            <begin position="14"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL30-like ferredoxin-like fold" evidence="6">
        <location>
            <begin position="91"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8210111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="248" mass="28720" checksum="D0DF7E1813243F73" modified="2022-10-12" version="1">MSQKKAQIQVEQKVPESIVKKTQRDSKLRDLVVKRRAERLGQNKARRAQLEKNAQAYEAEYKATEKSLIDNIRKAKTEGGFYVPAEAKLMLIIRIRGINTLNPQVRQTLRLLKLRQLHNAAFVRINKATIEMIRKVEPYITYGYPSRTVIKNLIYKRGYAKVNGQRIPLTQNTIIDQQLGKYGIHGIEDLIHEITTVGPHFKEANRFLWAFKLRGPRGGFIAKRKSFINQGDWGNREDLINDLAKRMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YCJ1</accession>
    <name>A0A8S1YCJ1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1630029</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000163">
        <property type="protein sequence ID" value="CAD8211331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YCJ1"/>
    <dbReference type="OrthoDB" id="301279at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF115">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="4" id="KW-0187">Copper transport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8211331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="139" mass="16329" checksum="0D580D8DFE6163C1" modified="2022-10-12" version="1">MSHTEQSEMHPGMQMMDMRMYMIINWDYKVRFVFNTWKSTTQGEFVGGLFITMFFCVFLCLLPVLKGFAERQKSLFLKIVWQLLAATQSSITMFLLMTVNGWVFLAMALGFGIGNFIFYPQMPISQKHQREDDQKVLNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YEK9</accession>
    <name>A0A8S1YEK9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1670033</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000167">
        <property type="protein sequence ID" value="CAD8212220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YEK9"/>
    <dbReference type="OrthoDB" id="293918at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035894951" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8212220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="156" mass="17747" checksum="04431A0303B564B4" modified="2022-10-12" version="1">MLLLFIFIVPLLSQTCFSNKAAVDCTYLKCCSQQGYCAINESECYYSAEKYSCDYAECDYCCIYNGDTVECGTQNQCLAWIFILLGSWLLMTLIIIFAAINRKRNVATRMIVIAEKAKLVALDNSRKSIVSQIIGTEEQQYGKEFQVNIDNYNLQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YFV6</accession>
    <name>A0A8S1YFV6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1670022</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000167">
        <property type="protein sequence ID" value="CAD8212198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YFV6"/>
    <dbReference type="OrthoDB" id="300467at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8212198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="529" mass="63101" checksum="B2F6AF8EB6B8B7B9" modified="2022-10-12" version="1">MINSSQLYLKREQFFIQIRKQAREEIFSRKRHTNLNSDLDGELKCLPDELIVQIYKSFMVKDFQSLKSLLFKYNEQLLLIIVKQQQGDEVMMNQYINHFSINQDATQLFMQILRMSDVQLDSNIEEIRVKCLNLVIIILINLTYGNTPQIINNLLNHNFLDIIFNDVLGRMNSKQKLAQNYDEWQLIIESLSQLLINLLLDLKDERGVQIKIEIMKSSFLNIWHYVYREFPPQILWSSMLFLQQMIFFNPSIPDTEIVSLSLQRILQQCDYVITELSEQTQLYEAALILINYCSKHQLETTIILTKPVWVKILLINKYPLVMYSTFINLTSKQYDEDSPISNLNVEKQLLENGILNNMLSWLQNQPDEQLIIKIYKCLNNILIYQYQHLANVVISHFLPFLDLYFEGTTYYQEEIVNEYLILLKNLIIFQKLNDKNVIQILEEYNIFKQIKEILLQGCFQTWLIMNLFEAFDLLSIQFKLLILNKMVQYQIVETFHLFISRNDISQDFIETLQEYIENWQSEIYELNNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YGH2</accession>
    <name>A0A8S1YGH2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1740048</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000174">
        <property type="protein sequence ID" value="CAD8213165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YGH2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="258" mass="31546" checksum="1D8363EB62E62038" modified="2022-10-12" version="1">MQLEQNAKMLMNVKSCLKYQTLILNVELEFKNLQLRLKEDLLRERNNCQDQDDKIHYVSNKKITNNQICFYLEGKYLETIFENAPEALIIYKEFRDFLSSSKTKYNFESFQRLLCQTYKYSQIVRQMLLEMIDFEQENNGLKCKLEFLEQIQNKSTLFRMLRYLYKKRKRLFRKQKLWSKPQYKIYIPSYIGYDQLKSKWIHKVSKQLRIFLGGIANANQKYEKMMLQISIHTNYVKNQEYLDYMIRLQMFNTDLHLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YIC8</accession>
    <name>A0A8S1YIC8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1920001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000192">
        <property type="protein sequence ID" value="CAD8213965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="4768852at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="195"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8213965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="242" mass="28153" checksum="F17838D1E1A66582" modified="2022-10-12" version="1" fragment="single">MSKSLVSYLKYLERKKFKTVCYFFQIMEIKKIWNNTFYRIQQRMIMDRNCMILEITSNKLLMSQKQQKIMASISQAIQIKNMKNLQRIQQKEQNNDRQIIEFLKFLVHLTSMDNTFIQCGSNSLHILVQMKVSLSNNNFENIKIQNISLVGANFFRCNFSGSKFNNVNKSGINLNQTLLLNCKWKNLRIHELNYVNGHTNAVKSVCFSPDGNTLASGSDDNSIRLWDVKTGQQKAKLDGHXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YIE1</accession>
    <name>A0A8S1YIE1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1750033</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000175">
        <property type="protein sequence ID" value="CAD8213211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YIE1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="206" mass="24736" checksum="DFB73A21149FEC12" modified="2022-10-12" version="1">MRVLFEEKIYKQEGNDLQEFFNSINQFQSTQIKEFKNDISKLLQQLKEPDILQTCDNILIHKQSLPYQFQEVTVEEESKKTNKWLDFIYQFRNQIYSVKNINQTEQFFQICSQRKQVLRVQLVINVLMSILHKIIKVLNLLINNGEYNDKLRKILILMKKYNQKQIKLLTIILEIPMKIKNSIQFCTIKLQNCILFVFLPLSLPYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RUB1</accession>
    <name>A0A8S1RUB1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0010174</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RUB1"/>
    <dbReference type="OrthoDB" id="285795at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45641:SF19">
        <property type="entry name" value="NEPHROCYSTIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45641">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_6G03870)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="311"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8131576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="410" mass="48087" checksum="163D8B55F867FDF7" modified="2022-10-12" version="1">MGDHFKIIDILIRKQQFEKSLNKLEKMQNELNAKLNNPEECSETELILQSNIYERYAQIFIDINQVYRAMQYLINMIKIEKELFKTQNDKESILRLCNSYAKIGRCCFYCCYYEQTFKYLDYAQSLLIKHNLTKTGVYAMTLTQLGNYYRFMFQDELAEQMLFESIKIREELFTRQSIEVADSLHGLSQLFSDEGKIDEAMKSISEAISIWTDVLGYQHIKTAKSIYLKGNLYLRMKNNQENLSSAEKLITESLEINLKIMGESSQDIADCYHSLGKIKAYNKNSNEFESYFQKSQQILKILYGQSHASIAIILNNFGRSYFERKQYEEAVNCFEESIKIYTQLCGKMHGNLAITLKNCADCHKELGRYKQAYIDYQKSLEIYQNMQINSSQANQIQNLMSQISDKYLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RYA4</accession>
    <name>A0A8S1RYA4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Histone-lysine N-methyltransferase, H3 lysine-79 specific</fullName>
            <ecNumber evidence="1">2.1.1.360</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Histone H3-K79 methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0020170</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Histone methyltransferase that specifically trimethylates histone H3 to form H3K79me3. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-lysyl(79)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(79)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60328"/>
            <dbReference type="Rhea" id="RHEA-COMP:15549"/>
            <dbReference type="Rhea" id="RHEA-COMP:15552"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.360"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">In contrast to other lysine histone methyltransferases, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.360" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8132986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RYA4"/>
    <dbReference type="OrthoDB" id="443402at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140956">
        <property type="term" value="F:histone H3K79 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025789">
        <property type="entry name" value="DOT1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030445">
        <property type="entry name" value="H3-K79_meTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21451:SF19">
        <property type="entry name" value="ACTIVATED IN BLOCKED UNFOLDED PROTEIN RESPONSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21451">
        <property type="entry name" value="HISTONE H3 METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08123">
        <property type="entry name" value="DOT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51569">
        <property type="entry name" value="DOT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DOT1" evidence="4">
        <location>
            <begin position="1"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="506"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="270"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="582"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="506"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU271113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8132986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="795" mass="93783" checksum="22D33C489211C319" modified="2022-10-12" version="1">MDHARILKFLNSLQNQAYMIKQEGRIDTQQTDYAQCPKESLKSLYEKFTKDYSLQRARTLSREERLANHLTSNSLSYGEIEFTSLSVVFQQVQPKKGGIFYDLGSGIGKSVIAASLMHQFDICKGIELLHSLHEQACKLKQEVEKQKTLIEDEMEQIGIKEYQQPKIEFINGDFQELDWSDGTFLFASTTCFEPDLMKQLRGILFYNCYQNTRTLDLLESYQIYLSAIVLGISYNSHIAKENELDLTIIKIIMKQSHSKKNITNSLYIANNQHTELIDYLKQENKQLTEQMNELKGLLQLNKKALRIMTPQNNDEQNRALLMVMKNLQEENAKLHNQIDKLIEERNQAQNQVLINQQITEEAQRHEKELILSLQNKIQTLQNNLNKTEQQLSKMEQLKPEYDEISGIVIKYKEICEPDKIAIKFHNQIEMLTFELVEQIKKNKLIEAEKQKLQGFNLKLMSNLVQMKNTAMTYHGNKLIIQQQQFQQGPEQLIKFQNNLLNVMNKQEDENSSQDDKNSSVGSSPLMSPLPLKQENQVVKQMKDIPKLDLTRAKQIQEINAKRQIQQMQDIKKQIDPNFVDKLNKYERIIEELRKNYQREMLLNKTLETANNQLQRNCDDLENQIQILITSNLRHQEKLQKINKQYYFLQQFYINNKDQITTTQSHNRRFSQSPTELSQEMILDTSFIDANGAIISNEIQEQKYLQQQMFEQQQQQQQFQQHFYSFYTPNNIEDSKKFLLSLAELIYCGLQDKIEQLQQQQQQYQQEKKQQRFRSVSDIIDYQYIGLQKYNYIQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RZP1</accession>
    <name>A0A8S1RZP1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Timeless N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0020128</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8132902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RZP1"/>
    <dbReference type="OrthoDB" id="310853at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031298">
        <property type="term" value="C:replication fork protection complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000076">
        <property type="term" value="P:DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043111">
        <property type="term" value="P:replication fork arrest"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044998">
        <property type="entry name" value="Timeless"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006906">
        <property type="entry name" value="Timeless_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22940:SF4">
        <property type="entry name" value="PROTEIN TIMELESS HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22940">
        <property type="entry name" value="TIMEOUT/TIMELESS-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04821">
        <property type="entry name" value="TIMELESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Timeless N-terminal" evidence="5">
        <location>
            <begin position="49"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="567"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1043"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="567"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="591"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="604"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1052"/>
            <end position="1069"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8132902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1069" mass="126706" checksum="91320EFB764EF3B8" modified="2022-10-12" version="1">MDRDFDSESNFEERLIEDEERNDQRYYTTQLLLSIQGLGTFTKVDNMNVYAKGEKAEGALKHLCQQCKKDIPDKPIARIYLAKYRAFQLNLINLLTCYPQDKVLSYYVVILMEQLTNIDRSGIKLQTSNAPQMLDTLQEYKLYFLKPIVLQTLVNHFAECIQIDQECRTKVHNDMIELIVLLFKNLLSIPDIEETKIGLESGKEVKRNLQRNFIYCMIKESVLSAFIYISQDFKSSFMQRIAIPLMEIFYYALTPFDPEWLLRDSEQEKELILKQAQQYRLQKQQHLSTIPNRHSRFPGLFVTKRVLSQSGQISSQPFHQHQVNKLANQRQKPEVRALISKQKTIFVKPLTIDLYDAEKEKSFKEELRYSTIDFLIHCFSKLIDQAFIEMNKEIVDSDDKVHYFVLISHFLKFIRFHCKQNKEDNQGQLSSEFVSVQAALQITQFNFIYSNLINESTQLRKKNFNYRIFFAVINVLFEFLMIVRELSLSVEQTDRRNSQILLQTIVTQDIAKAIQIGYGYCQFGLFNDQMLSTLVKLTALYYDLLELHTQGKAWSIRTNKLLKKKIKKQQNKANKGRKRKQDQPNEEDNDFIEKGPDEENPMREEEDDDENEEEDDENETEDDQPIYKERRCNFVSEFAIVVDYKVLEKYFYLIKEDKLLRNSSELNIAIYKYFKRIVDTLKADWIFFQLDFLYVINSIIQNKQLTYKSEFLNLIQLFRRIGISFVQLFKVNRLMSVEILFRFPNAATKDSILKNYQDSVAEGDTNQDNYQYEGPKKKVIWTQKEDKILIENYEKYKDCESPEEQLSLLLSQNGFTIKSPAEIRKRIRKFRLEEGNERAIALYQQQYEMISMKKVEIMAKSVKSLLQEVGEESLNLFFNNLFSTLQNYIDYRGQESIEMPIPPLDKLQFEYFEKGSVQAIFLGLDLKPPSQGEIYWRLQTHTFHAEINEMRSTLNKYLEKAKKIDDDQIIEEFQPLQINSQKKQSDSKKKVVENIPTYAEDEFDDEKEKQVSDKKVVEKKMKLNKKQKELDSEEEREIYEKYMEQQAQNEQIQKDVENKSKDNYDDIFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S6N1</accession>
    <name>A0A8S1S6N1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0030462</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S6N1"/>
    <dbReference type="OrthoDB" id="284480at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24126">
        <property type="entry name" value="ANKYRIN REPEAT, PH AND SEC7 DOMAIN CONTAINING PROTEIN SECG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24126:SF61">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_2, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="84"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8134719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="167" mass="19364" checksum="69103FD9CE9574A7" modified="2022-10-12" version="1">MQQEEEQYSCLEEFFIDSCRFGDYEDAIGCFEDNVDLFWIDINQNNCIHMVSANGHLSILEQIIQYSKKINIDLYKLINHQNQYGCTPLHWAVFNNQLAIIPVLLQNGADIKIKNLNGLTCIEEAIQLDKTDVIELLSKNISLTEDQQQTFNETTDIIEQEEEDQVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SAI7</accession>
    <name>A0A8S1SAI7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TmcB/TmcC TPR repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0050092</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="542352at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057352">
        <property type="entry name" value="TPR_TmcB/C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25474">
        <property type="entry name" value="TPR_TmcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="189"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="289"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1232"/>
            <end position="1255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1333"/>
            <end position="1357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1577"/>
            <end position="1599"/>
        </location>
    </feature>
    <feature type="domain" description="TmcB/TmcC TPR repeats" evidence="2">
        <location>
            <begin position="495"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8136322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1643" mass="192599" checksum="139D217AFCB9E0EB" modified="2022-10-12" version="1">MESKSNYNNAQTQLDNEGSFVDKLTLEMKQLIFKIVTLMLKIESQSVVFQLLMRLIQYMQLNAILFNRQIWKIWQVTTVSQPLHTFFNYFMITPYFDKDSFSYFVVLMYVCLSLFLITLMLICVIGFYSNKQNQSFTWAIAILRTLIEFFLSILFLPFVDVFLAMLACYDDGTGYFQHYLFDVQCWANAHIVHGIVAIIGVIIFSFITLLFSMVYYEVNYLPQNLNSKKQSKCTTYLLLYELVMVICYTYMNDKFYEYVHILIMLIGSFLVFWCFHVEKPYNNHIIQKSYSMLVTFHLWGVSMLCFAIYLEGVLFFGLIFSWMGGLPLLVITLLRPQNEQYEILMTNLATCQQPQQIYNLTNFLLQLQYESQNDQTSGLVIDGFLDHHKITCIRDDCHLKLKGLPNYRRNKIGLNDSNLQERDVDLSIVLYQMYLNQTKKFQNCIKLRLRFAIYLYEKMKQKQNALIELQAIEQLFPKFDEEFIIYRFKKLIEDELNSNEGYGSFDIGNELQFQNNYKTLQRYIERSSLLHMDFWSLLQEDFPDLSKLNELGQKINNSIVQIEDLWKKMQKQSTNLTKALRLYGKFQIEVLQDKEQGEELLEKSRIIQQQVGLNRNKATTSFINAEDIGLEALPTIVIATTVDKFSQITNLNKACCNVLAYNKSELLNKKITLIMPNVFAKFHDRYIEKFMTLNVSSLFNVDRYIFVKQKHNYIMPSFLNIRMLQSYDDSTMIVGQFKLIKQFKPICYLLTDNEKIIESISASCFSLLGIDNKLITHNKIYINDLFPQFQDQFNSFFTKQGAPIKFYNSLQQTKSPSYQPSSEQQTEKQKSDKFYQIYQCNVSEIRDFEKQLVGYSIKLELIQTEKSFGTQQNMSLLAEKQQTEQLVYNQMQFKFIPKAMTFVAEAGNEINSQRVDQSVNWDQQDQSSQLTSNLGMEYQKPSTEKSDELQIFTHSLKLDDGIKTLRLFENKIQDIEDREDIISEDEDSGKSSVFQKIDNDSIEVGANDNLNVFRSRTNLLQLINIQQTPKVITKLNWTMNLEMLSIVTLSFVAFFLTNQQYNKISDQLYLVQQVGIRNAEFFNLMAATQNLQMQQIGIWTFTSDSESKLYEKDQRKLINDTINTINEINTILTLNSDDDNEQLKELYTQNVVNMQVSEGVFQKYDLVQAMQQLLSKSLILRDKQLAYMTPKDEDFKFLTYNLLNDFALHLQNSTQQYSLDLAQKTADNRQELISILGTSSGILGVSLLLLIFFLIQMARGLQEILVLFLDIPDKTIKFLYSKCENFLSNIQIGEDDEMLSDFDELEKEEREELNKTLKQRRKKKKYKNSNKELRNLILISLILTILFQSYFIIFYFLSSQMLTNLSQMVPEINSTSYCEGFYKFVEGSERHLFLNRDIPITSVDSYYLLKVLIDALYQIDSYFHQEHSLNIDILNNIYIDSFQEIFMLQPCAIFASEMQTVTEEECVQFASGAIDQGMAVGLARFIENIRFLLTIYEQFYGHPEVSFSSAVRGNIVFKNITQNDDNVTNYIYNLNNFKQAKELRVMQSTYLKATFRYLFLKLKEGINLDMNNSKTQLLALFIIFEAILFILYFVFWLPLTIKLIRDIQKTRIMVMMIPLKVILKIRSIKLYIKDVILERAVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SC75</accession>
    <name>A0A8S1SC75_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0070224</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the OSBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SC75"/>
    <dbReference type="OrthoDB" id="309527at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13293">
        <property type="entry name" value="PH_CpORP2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000615">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000037">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF205">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8138641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="606" mass="71732" checksum="61DDB5319D1E830B" modified="2022-10-12" version="1">MEGYLLKWVNPFQRWQKRYFILNDHILTYCDQPGGRSKGQIHLKVAGINESKDDALRIIINTGTGQILLKASNVDEKNKWLLALKKNQEYCQRHQVFNYGQRIQELLTDIWTNFASFDEQLTYLKEKVSLNMQKEINDIINLGQYLKNGITLCCTLIEEEKIKLYGESDTIYESFDFENEIPLNTQTSNYTIRQEEQKQNQQENFNSYQFLETIKLSNPIKYNNIKYNRVYSRLSITNEATRKCLPYKQDPDEKFAFWPFLKECIGKDLTRIPMPLLFHQPLSALQFFASSFEYYETLKQAAKAEDPYKRMAYLMAFSLVRCILGIDCQKKFFNPVLGETYEFFTPEYKIISEQVCHHPPITAVHCESEDFIFSITMEATVGFSGTSIMAKLPGLVHFRNKKTNEYMTYSLPSIIVKNLLFGKMYFEQVGEAIYTNHTTGDIGILTLKERTSEKDAHQVKAVIKDKNGNIRCQISGHFNKEIYANNELIWKRNSVDPESKWYYFYSQHILQLNHLNEDILRSIPQTDSRMRSDMRALECGFKDLGQEEKVRIEEKQRERRKYMEENKLQHIPRFFKEEFDPISKRNQWVYLYNYEKEKHLIDLDLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SCR5</accession>
    <name>A0A8S1SCR5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0050213</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SCR5"/>
    <dbReference type="OrthoDB" id="10354388at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="167"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8136564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="360" mass="43428" checksum="2DEA248B1F79BD55" modified="2022-10-12" version="1">MQLTKENIEFYIQRRKQYCLDFLQQTNQFKPKLAQLQKRLIATKQGIDGPIKSISHQQKIKKVNKVLASLSKSKEVQNKIDKEMIRCQCQYELDEVPNCNFNYSQTCNKIQQAMIPLIKNLQIQIKGKQNEISSDQDCADLYIKKGEFQQMQHKSKRLQQIIEKLHIMELQQEEKVYRESIKKLEKFDQDEEYDETLPYQTLLSKRRLEAKVLIDEERKQKKQIENERIKENIEEQKQKYHLLDNKIKIHYRKIGIQKGKLESMSEQMNDQKTITQINVDSQNSVKKQLLKSILKADKRFSLTLFSTQLNNSNIPHQYQQYQQYIKKYQKQYWKKYILVFQQDRKKISYLRIIIRKQIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SDJ9</accession>
    <name>A0A8S1SDJ9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0060419</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8138038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SDJ9"/>
    <dbReference type="OrthoDB" id="287430at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018943">
        <property type="entry name" value="Oligosaccaryltransferase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10215">
        <property type="entry name" value="Ost4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8138038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="35" mass="4002" checksum="0628915A152A1F4D" modified="2022-10-12" version="1">MEYEIMLLCNTLGIGIVVMIILYHLIGTKADKKTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SF13</accession>
    <name>A0A8S1SF13_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0080266</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="445034at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051242">
        <property type="entry name" value="WD-EF-hand_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44324">
        <property type="entry name" value="WD40 REPEAT DOMAIN 95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44324:SF4">
        <property type="entry name" value="WD40 REPEAT DOMAIN 95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="87"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8139731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="308" mass="36106" checksum="C314F1FECEA43195" modified="2022-10-12" version="1" fragment="single">MTTQIKKLSKLDHTVPIYSPRTTYKQILHTDETLLADLTLNFDPFTINVLRTEFLMRHGSMEVTEFILVVKEHLLSWQLEISNRDIKLIRQLVNLFEEIDLNGNGNLEWEEFTNYIIEKATVLNNIKSKQDEIKLYTKSNMKPFKKFTQIITRVIYIKEIDKIAFFEEGSDEIQFMTPDGGFGLKPLKIVPISDKVVTTQAKKDKDKQNFVIVKKEDTIEKKTQILAMLYIDDPKYQILLTSTHDGYVRGWRYQSSGFVQANQPDNDEEMIEHHFNNDIYSMTWDGINEILYCGQKEGQINIWNLKXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJM8</accession>
    <name>A0A8S1SJM8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0070405</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJM8"/>
    <dbReference type="OrthoDB" id="291953at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000465">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="170"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="203"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="236"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="423"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="25"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="272"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="25"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="74"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="93"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="459"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8138972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="736" mass="84127" checksum="D9769AB79C321C74" modified="2022-10-12" version="1">MNINSNSKPSLQEYLDKLKQIQDANNRRLSQAKSQTNASKIMTPKTTSQLKQQTKPSTDRSHSQHKLMPFFQNRKVSSSKRQTSQADEPKIPISSQGITKTVEATSPRTDELEDIKKQLNQWFIKESKNNNTGGCLQLLEPTLVHNILKLPPRQKINSELRASLSAKDMLDNTALHYAAKNGNAQLTSALIFKQISIDAQNKDHMTPLILAAINGQDEVLMILMNAGSDINHQDSLGNTALHYACKQNHKTIVQLLLKRQSIQFKINKEHKIAEQYAQNEEIQRLFQNYNEDIKKQQQKMNMVQIQNTQNDVILKMFQFKRGQMNQQQQQQQQQQQQQGIQLQTQQNFAQTPTQLNSYSCKNLNVVNKQSSSPNNRTQQEIKQERLNKINSTHSAKQQLTNTVSTNSDSIKNKEEEKIGPQQFQVIGLIGKGSFGEVYLVQKSNQLYAMKVLHKSRIMKHNLTRYALTERNVLSITSHPFIVKLRFAFQTQDKLFMILDYCPGGDLGEVLQKQKRLPENIVKNYLCEIVLALEDLHKRDIIFRDLKPDNIVLDSEGHALLTDFGLSKEGILEPNTGARSFCGSVAYLAPEMLKRSGHGKAVDWYLLGVVMYELLVGLPPYYANNREELFYNIENAQLKIPSYISNEAKNLLKALLQRNPAKRLGSGKGDSEEIKAHQYFQDVNWDVVYNRELTMPKPNRKIRINTKIDGNVFDMQSIVEESKAHLGGWTFVNNDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJX3</accession>
    <name>A0A8S1SJX3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Membrane insertase YidC/Oxa/ALB C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0080467</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the OXA1/ALB3/YidC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8140130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJX3"/>
    <dbReference type="OrthoDB" id="2148490at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051205">
        <property type="term" value="P:protein insertion into membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20069">
        <property type="entry name" value="5TM_Oxa1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028055">
        <property type="entry name" value="YidC/Oxa/ALB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428:SF14">
        <property type="entry name" value="ALBINO3-LIKE PROTEIN 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="OXA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02096">
        <property type="entry name" value="60KD_IMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="198"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Membrane insertase YidC/Oxa/ALB C-terminal" evidence="8">
        <location>
            <begin position="75"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="317"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="326"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8140130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="360" mass="41417" checksum="D74B850507459D5A" modified="2022-10-12" version="1">MISRTLKLYKFSTKIPTGVFDFANVQQGITAPSSIMVEDLITRFSAYTPTNMYNSLDEYSRELLLYIANDLSWGMGWGILAISMGIKIAFTPLMFSAQLNACRMKLIEPESKNFQNMIQRAMRAQDYKASRAAQKQFKLFKRKHNINMLIPGLSILQMPFLFTWFLSLRYVCSLPDKYEGLKSQGFLWFQDLSEYDPYGILPIMSSFFTFWNISLNPNMQSQSTVPFAKYYRYVRFLPFFSIPVVIFFPAGVNLYWCSSAFCHLIITALARQEKIRKVFGIPKYLPGTILERQNNMNIQTIVKAVVQDRADLNSDVTTTTTATTTPPQKQVQTDAQHIMHEAKSGEQKVKIFSSKPKKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-02-22" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SNV4</accession>
    <name>A0A8S1SNV4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0110011</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8142203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="137" mass="16024" checksum="4C98570514799DFF" modified="2022-10-12" version="1">MYSYKPKNDQIEEKQRVKSQFQISRIEQVIQEESDNKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLVQQQNKVQTRRQVCFGISQKESVTSIFIHFLNWNLELYSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SP32</accession>
    <name>A0A8S1SP32_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thioredoxin reductase</fullName>
            <ecNumber evidence="3">1.8.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPENT_87.1.T0100181</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the transfer of electrons from NADPH to thioredoxins TRX1, TRX2 and TRX3, which in turn act as reductants of disulfide containing proteins. Able to reduce nitroglutathione (GSNO), a compound involved in the transport of nitric oxide (NO); however, TRX1 is more efficient in reducing GSNO. Has no catalytic activity towards oxidized glutathione (GSSG).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.9" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SP32"/>
    <dbReference type="OrthoDB" id="5956163at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004362">
        <property type="term" value="F:glutathione-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000190">
        <property type="entry name" value="Thioredoxin reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.30:FF:000004">
        <property type="entry name" value="Thioredoxin reductase 1, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046952">
        <property type="entry name" value="GSHR/TRXR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006338">
        <property type="entry name" value="Thioredoxin/glutathione_Rdtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01438">
        <property type="entry name" value="TGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737">
        <property type="entry name" value="GLUTATHIONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737:SF7">
        <property type="entry name" value="THIOREDOXIN-DISULFIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 12" id="KW-0274">FAD</keyword>
    <keyword evidence="5 12" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10 12" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="14">
        <location>
            <begin position="7"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="13">
        <location>
            <begin position="360"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8141538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="486" mass="53877" checksum="5B7A3ED2F5F7DFF3" modified="2022-10-12" version="1">MDVEQQYDLFVIGGGSGGLACAKQAAKLGVKVALADFVKPSPLGTKWGLGGTCVNVGCIPKKLMHFAATLGELRHDQVEAGWIDTQIDSKHNWEKMVENVNNHIRKLNFGYKNQLLRQDVKYYNKFAELIDANTIKLTDNKGQIETVRAKNILIAVGGRPSYPDNIPNIQNLVITSDDLFWLQENPGKTLVVGASYVALECGGFLNGIGNEVTIMVRSILLRGFDQEMAVKIGYYMEEKGIRFIRGCVPDMIEATEDNKRKVTWIINGNKQEEIFDTVLVATGRVSDTQKLNLEKVGVNMNKNGKIICSADDKTSIPNIFAIGDCVEGRPELTPTAIKCGQLLANRLFNKATELMSYEFIATTVFTPLEYGCIGFSEEDAIKKFGYDKIIVYHSIFKPLEWNYFEMHSGESCFAKLIVLNDNRRVIGFHYLGPHAGEVTQGYAVAMKMGVTKEQFDSTVGIHPTCSEELVQVTAVKGIDDAQKEGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SPE1</accession>
    <name>A0A8S1SPE1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0090016</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SPE1"/>
    <dbReference type="OrthoDB" id="301354at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8140324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="242" mass="28134" checksum="6E19B975916E34E9" modified="2022-10-12" version="1">MSAIILLIPFLFNLEDYILYYDFASLLCVQQWIFETNSQFKFFESLLLTVVIMFAQSFTLNLIYFDENLFSNSDNIYQACLYIGLTIILANPISIKFQDRIAAKYEPFPYSVMMIDKVGQALTISKLIEAFIFDKIPFTNLSFILSTLLMSQIPIIVLFIGLTDYHSTKTSYSKIKELALENSIIYLLTSSIFLYLKHSNIVKLDSQIYQFLSIIQFVLYSTIFYQVKKAVKSRNQSKIKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SQ34</accession>
    <name>A0A8S1SQ34_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0100383</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SQ34"/>
    <dbReference type="OrthoDB" id="299183at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="271"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8141938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="310" mass="35850" checksum="4D83C5D1F4909264" modified="2022-10-12" version="1">MDQVCQLHRNQTRNKESLSYRRINSPGLPNSSLRSSCKGDKKRLEIGLITDRKLSESPNFDRMFSTGNFKIQQQKNSIIDIPQPQSQVPKMEPPVKRNLRMIEKLESDKLCLKIYVSEEDQNQLIFTGISKEKLQSPKQNKKALIKNLQQLFQKGIKDQNKYIKLESLEKQVNLENQQYYGLLKNNNNESSKRIITQNNESKSNLDKSLSISTIQEYKSNTSSKINKDLEILENGLNKQKNYQQTYSNSKFQKSQPLGTYNLTINLKQKQKDCKISSQNKKGPSPSFSQQRNSQQKCINKMKSNAKSLHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SQ80</accession>
    <name>A0A8S1SQ80_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription and mRNA export factor ENY2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Enhancer of yellow 2 transcription factor homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0100464</name>
        <name evidence="3" type="ORF">PPENT_87.1.T0130429</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in mRNA export coupled transcription activation by association with both the TREX-2 and the SAGA complexes. The transcription regulatory histone acetylation (HAT) complex SAGA is a multiprotein complex that activates transcription by remodeling chromatin and mediating histone acetylation and deubiquitination. Within the SAGA complex, participates to a subcomplex that specifically deubiquitinates histones. The SAGA complex is recruited to specific gene promoters by activators, where it is required for transcription. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the nuclear pore complex (NPC)-associated TREX-2 complex (transcription and export complex 2). Component of the SAGA transcription coactivator-HAT complex. Within the SAGA complex, participates to a subcomplex of SAGA called the DUB module (deubiquitination module).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ENY2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8142100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SQ80"/>
    <dbReference type="OrthoDB" id="6221744at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071819">
        <property type="term" value="C:DUBm complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070390">
        <property type="term" value="C:transcription export complex 2"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03046">
        <property type="entry name" value="ENY2_Sus1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018783">
        <property type="entry name" value="TF_ENY2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12514">
        <property type="entry name" value="ENHANCER OF YELLOW 2 TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10163">
        <property type="entry name" value="EnY2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0010">Activator</keyword>
    <keyword evidence="1" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="1" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1" id="KW-0811">Translocation</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8142100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8144844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="103" mass="12391" checksum="5C4C0650D195509A" modified="2022-10-12" version="1">MNQQSRLAGDFINQRTNLLAQKLRETGEDSRLEEYLRQKLIECRWRDDLKDYCKEVIRQKGLEKITIEELTDMLYVRGQATIPNKVKEDLLSRLRQFFDENQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ST88</accession>
    <name>A0A8S1ST88_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cyclin-dependent kinase 2 homolog</fullName>
            <ecNumber evidence="2">2.7.11.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cell division control protein 2 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">cdc2-related kinase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPENT_87.1.T0110251</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">May form a complex composed of at least the catalytic subunit CRK2 and a cyclin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ST88"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010389">
        <property type="term" value="P:regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001126">
        <property type="entry name" value="Cell Division Cycle related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF254">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="10"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8142616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="300" mass="34909" checksum="3AC24EF6F6B07E39" modified="2022-10-12" version="1">MQDNKKIRQFILQEKVGYGAFGDVYKVIDENTNEVFAIKRIFSGKSQQIDQSTIREISALSSLKGQNHIVPLQQVIFEKGCVYIVLPFYPYNLYGFIKKYNEINYKKIFKQLILGVYNIHKLGFMHRDLKPLNILVDENQNVYISDFGIARMNFGQCRNFNSNCEKHSYEVITLHFRPPENLLGCDNYHVSVDVWSLGCVFYQVVMKEILFYGDSQIEMLFKIFSKLGTPSAFIAPSLCSLPLFNEAAAFPRFYPTKYLFQQELLSKIGYEGADLIDRMIQLEPSRRITMSQALQHQYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SUL3</accession>
    <name>A0A8S1SUL3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0120239</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SUL3"/>
    <dbReference type="OrthoDB" id="303113at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8143458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="565" mass="66445" checksum="1B61B73705BDC810" modified="2022-10-12" version="1">MFNKYSLTFQSANLELEYRESQIPVVKKEYTAFSLQACLYMIFQSIYYLRSQPQFLAVLLSCCFVVIFIIFSKFLIDKYPSVINIILPILQIIMVLIQSVDYVFRESNPNNIYNSSYDWYYGFCACYFHMAIFIQGYLLLSQAFIVIGLLLVYILWAYNSSKVYISLLLSFLSIIVGIIAIKYSIENNKRQQFLHNRERKKWFAIIDTVLEQSIIVIKLDKQQDQLRIHQMNNITKEILQVNNDNDLRTVLRNLTYSRGSKLSGEDNKCSLEKHIRMMLQSNTSSGSAFELRKKGSQDKLINSLICQSNILEYQFKVQIVNYWTDEQQMVIMATECLNNNQQNHDKLELNLKNRLITSLAKHCLNVQFKEKNDRFSQLQCMNYFVCLNILQNPNILNGIHIKKYSSEQVIKLLKSVFGNHLEIINKLKIQYINLDLNSVLLIFITLLQFREKKNGYIKISSKINEDNIESIHFTLMQSTKQIIPQIRVYFTKPLFFFQKQDKSTNLSHLQQNLLHLDKKLNCQDPLTATTLIIMKFLLWHFGCNTQIKTKQKNNLTYFSFSLLNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SVQ4</accession>
    <name>A0A8S1SVQ4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0110219</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SVQ4"/>
    <dbReference type="OrthoDB" id="310439at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="52"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8142552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="476" mass="57127" checksum="43F3DE29BDFD844A" modified="2022-10-12" version="1">MQQQQTTNNFTTQNKLRESGNDKNKTIQEEATQNFKQEDILKDVTNKLQFKILKKNEQLTQMKEEIIDLQVKWQEDKKDWLNEKSELERKCEFYKENLNAERQNNQKFQIQIDKLTSMLNQKMKGKLQYNESSALEKVSGQLNEEIDSLQKETGIMKTQICLLENKLILKDETISKQQKRIQKLKKKLDQQLQQQLEERMVDVDKQKKRSADIKERVSELELKLKNLSLESDRIKKDNKQLLQENDIFRIQYEKSSRLLPQLEDSNKRLEQEIQEYESQFEKMSKQIKELNNQFVNEQQQQTSINEELKDKNLEIDNLQEQLNEQIQLFHIEQRSNTFLKEESEKYKFQLDRLTKQQQQLTDAQHKDLDNLEAKIEKISKELIKLREENTLLKSQVQNHKKEQGQYLDMIDQLQKQIRDLKGKNKILVQQHDDLDARINQLVQMKKQKCLKVRIQDTNQQKKFTRFEKSDSSSFSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SZR7</accession>
    <name>A0A8S1SZR7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0140395</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SZR7"/>
    <dbReference type="OrthoDB" id="10609904at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="253"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8145720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="415" mass="49928" checksum="E2CDFBB9C5C97561" modified="2022-10-12" version="1">MNNNQTFWTLLVIQINKLLILKLNIASISFNKSINSFDVDSLKFNIDPQKQEDHINKILIYCKTENLEQNLAYRMRIKSFLCQLGEFYFFLDVKNVNQNKDLILLLIIVQNVLFLIKINLKPQHLIKFNQNLDLGGLVRLLIMQNYAIKIEVFVWEDGILAMIYVIKDIQEDCVKNVIDLMQEEKDNSLKTINNWIVNRNNSFFLISIWAILLLYQLVKNIEKTNQLYVYLKPRSNQNFAEFLFKLNQDHESILLKLFLNYFCVFSLIFTFNIYFSISLDFVKPSNDTSFLWLIILNIFQLKFKLSNQFIVEFQQCSDLCFLDINYLFWILTTINFRIELYLKLCILDQLEKLLYFLDLFQFWQFEKYQIKTIYQVMNLQFMNLKIISHGFMDFQFLDILTQQNKIQKTYRIFIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T1D4</accession>
    <name>A0A8S1T1D4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0140198</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T1D4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8145349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="45" mass="5289" checksum="1F559B4A4508B676" modified="2022-10-12" version="1">MIHEKTIFVFNLFEVKKGFLFRTTQLSNINSNYQKSSAKPINLQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T1S9</accession>
    <name>A0A8S1T1S9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0140232</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T1S9"/>
    <dbReference type="OrthoDB" id="299447at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8145416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="162" mass="18714" checksum="E4EB87D8B249859A" modified="2022-10-12" version="1">MSAKTIKSPSHKCFPTTRGHEIKIISKIDCFITTNTKMTQSCKQQGEHHKDLNQNKTVSIKAQKKNSQNSDKEALISQLLLKNLKLKEEGDKKNKLIEMLIDDRTEIKRVTSLHLPQEKDNQQSKANFRLLSPNHLEFTFYKSPSKGLPKEFQITPSKKMFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T5D1</accession>
    <name>A0A8S1T5D1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0180124</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T5D1"/>
    <dbReference type="OrthoDB" id="291754at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8148555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="855" mass="98577" checksum="A3ACBA5265713241" modified="2022-10-12" version="1">MDQIIELIQWSAQPDNQIQKQVYKRAQELSQDTQFLIHLCSVIIGKYDAEIRYRAAVILKSAIKNCSTLPEQLQQMLIQVDINIQIMRQAFIIIVPEVVVRNGIKNSNIMMEYLIKLIDIDPVIATDCLSKIIEDLKFNSENINYYGTDSGLQLIDQLILKFISLVEHTNTQVVVNSLNFLNYNIFFMPPSLNQYIDCYIKILITGTQSQEQQIRLKCFQGIQALIETKRDKIKQMNLIIMACVQSLQDPDKEVVRFVELCLTDFLRIEDAEDYEQIHLLEPYLSSILSPIILNLAITRADQIAIQPTFSNSYNQNGKNDDEEQDEEKTLGEYSLRSVSNLLLKKLIEFYDKIVVPIVLQIIDHLQQQQDWKQQEIAVICLGLFAEKIMENQGNLVPNILMSLFNEKNQQNEYIYASTLWTFSQYNEWIKTVAINETQFIQSYLKLLLISIENQSIIVKESACSALNSLSKDAFFILKPYLLDLFQVYLKALSQKGGVLLYIYQSITTILAECETIENQELIDLIMTKLISNLVDLNDYNICPLYECLSEAVEKFGQRSIKYIPIIYQAIVQSMNGYVQSIQNGKTRLLYQQKEILKRSFDLCIKIINITKERFLELCDQSFLQIVDLGLQDSENDVKQYALSLIGDLIKDCYIIFKNVNIAIVLNEYIYAQSISIDPSKLFLATSNNAAWALGELAIKDPTKITVIFNAVMEKLIKIINEPKFPKSIAQNLCIAICRIAGSHMQQIEELIPNFFKRVCLILSQIKSQSLDEYKEESFKILINIVKMYPGRVINDIKYFVYCIVVSTEFPSIKPLFVNILQELQQSFGQQKFDSMFSTDDLPKGFRMKMINIYGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T8C0</accession>
    <name>A0A8S1T8C0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0170063</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T8C0"/>
    <dbReference type="OrthoDB" id="300867at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8147636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="329" mass="38339" checksum="F0A06EBAC18DE0A0" modified="2022-10-12" version="1">MFQNSHTNNQFSFHSTIKFKKLPKLEPLFKQINQVVPQNTQRGRSFKYYLFNVTGGQCKFRRKTCTCSECGGTNTFVEKQNALQPITYIRAHHVKKRKRKMHRNKGGGRHLSAMITRVPLKIEDITNLIPTRKERKAKTLLQNQFIEQTLLKCKRLLTKDDSTIGYEYFFANELKIAYQTQEFMTNSTFEKTPTQIKQQNGNLTGSQRTIDESQVTIAQNLNINQSQVLNIQLPKINYKIKQKPSLYSPRIYQDILESHNLKPNKTVDKYISQSTHCSPVNIKLPLISQKKVIQTKRKKSEHVYLKGSPLKLLHELKSQILLQKKMSKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T933</accession>
    <name>A0A8S1T933_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Trimethylguanosine synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0170067</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T933"/>
    <dbReference type="OrthoDB" id="194443at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071164">
        <property type="term" value="F:RNA cap trimethylguanosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019012">
        <property type="entry name" value="RNA_cap_Gua-N2-MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14741">
        <property type="entry name" value="S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14741:SF32">
        <property type="entry name" value="TRIMETHYLGUANOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09445">
        <property type="entry name" value="Methyltransf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8147644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="485" mass="55955" checksum="346618595DED21ED" modified="2022-10-12" version="1">MSNNIKNSHKLEDVYDDQGYFCGCFKKISNMEQNRTIQTSKVRNSMNKSGLDGSKHYASSQLNIINSTNSQSQRQNVSVASPIVSSFAITTFENNYSQNQLFLLRNTQSGESIFAKITQIDLKTKKIQTSDLKKSRLFEQKIDNEIIQKSQFQDLTAKYIACRIKDQITTLAEYGCGNGGNTVQFTKYLDFVIAIDINTDACFQTKQNCDQSAFNLELPNPKVEIINADIFKLKKNLPFDSIFINPTMNNEQQICKDIIKDCSPNLKELLNLISDQIENLIIQFPAQIDYSQLPLLLNINQQYRNTNKQQSHCSLEIEKVYIQGTHVYNIVYYGKVANVSREELKQLLTTQLTNQETNKDGFYQVIAQLNEKIGNLQLTYELLQAQSYKYNFDQFLIAVCEKYKLDYNDYAFLLYQKLSKDYSQNSKFRDSNNVIHESEEFEFSQKYSYSLTGLSQKEEVCILESQDLYNFDLVGISNEKLNLCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TEQ9</accession>
    <name>A0A8S1TEQ9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phorbol-ester/DAG-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0230098</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TEQ9"/>
    <dbReference type="OrthoDB" id="299237at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15489">
        <property type="entry name" value="PHD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028941">
        <property type="entry name" value="WHIM2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15613">
        <property type="entry name" value="WSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="8">
        <location>
            <begin position="778"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="753"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="698"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="753"/>
            <end position="764"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8152531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="840" mass="99767" checksum="410556C2FFD57AC1" modified="2022-10-12" version="1">MLNLDQQELIIQRIKSKKTIFKTVCETEQFPKLIKSREAIQKSILSQIQRSVSNIDMDPLAYGQKELKDVMNYLEEQLKTVFLPFLSLDERKKSKCCIKQESKQPDYEESNHPKKQRVDKNKCRKCKQSTIPMILNLLQQTENQDTNPHVTMKSIRLQIVQKSQKKEEQSESKVPYIAFDYSDRIIICKRCRFKFHAECVQVLDNINDWICDYCLDRIQELKNSNQWDYINNKQLKQQICPTSEQKKINLSLIERIQKKQKIFIIEEMEITPFKPIDQISDFLRKYPLYRGQNGKIKYPVLEKLALDYPEVLEIKQKPQPYMINSNLVEEILKIQTSYQILFPQCQTPQNVNQDYIEKNFYEILMNLLNEYFNEFMQSDVVKFDKYCSQANVQANAFLEIMNYLYDHQENLIKEDLIKKTWVEFVIQIDQIINYDCESNINKIDFNSLEFSDQIYILNILTEGLYDLDKMKEQIKQKDSSNNYRQSHLKILFQQNQSAYINCGTYLGQDADEQQYFYFTHVPSSIFVETMTGWGQYTLSDLSELMSALNLQGVNESNLFENLELISQVKLFDNTTPIKSQNRLIQKQQYHLEIIEVIEKLLITEETYTKYLQSKNLFWIENNKKQSFYQLLKNEKQIESLIQALKQFFNGLLLLKSNDGYFVKPFKIFKQNFELVNQLAIYVDNAQSQYSVYISLITLNLLLEEYQQYQSQKELKNKKNQAQRKITQKVNQKEIKKIKKKSKLKHIEDESIEQEQSLQHANSLANKREKRENAGKNPNINFDQQVKNQKKIFCTSCKKQFPQITQSVQCSRCNKPFHQECLSIRKDYCKNCVRNGSKKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TK06</accession>
    <name>A0A8S1TK06_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0220252</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8152047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TK06"/>
    <dbReference type="OrthoDB" id="538223at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF8">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="19"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="62"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="104"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="238"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="284"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8152047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="317" mass="36238" checksum="E16B07369B7FFDBD" modified="2022-10-12" version="1">MQPQENWLDNSIEPFQKFEQAHANSIECLQFNPSNQSELATGSHDKLIKLWDVTKQKELSKFSGHKEGIWSLSYSLDGKQIFSGSPDKSILVWDAKSGKNVQALKEHKNRIYWIQVSDNGLYLASGGQDGHLILWDLRKLKLIKDLQISMDIVYNINFSQQSKYFFTGDSMGIIKAYDSQKIEEISNTKPTQKNKCYAIQSLKISEDNYKLFVASKNQSISEYNFDGKKKELTKINQSPVHCDSVHCLNFDKDKGRFGTGARDGAARVWQTERNGVVYNPLFNLIGHKQRVTAIEFHGTGKMIATCSWDQNIHLYKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TLS3</accession>
    <name>A0A8S1TLS3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FGFR1 oncogene partner (FOP) N-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0220218</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8151979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TLS3"/>
    <dbReference type="OrthoDB" id="2160638at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034453">
        <property type="term" value="P:microtubule anchoring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018993">
        <property type="entry name" value="FOP_dimerisation-dom_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15431">
        <property type="entry name" value="FGFR1 ONCOGENE PARTNER/LISH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15431:SF4">
        <property type="entry name" value="PROTEIN TONNEAU 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09398">
        <property type="entry name" value="FOP_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FGFR1 oncogene partner (FOP) N-terminal dimerisation" evidence="4">
        <location>
            <begin position="47"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="127"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="127"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="152"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="162"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="242"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="274"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="300"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="319"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8151979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="363" mass="43990" checksum="74BACDFC0CB29B2D" modified="2022-10-12" version="1">MQELTELKNLVIQSLEANGSLAKIRAQIRASVFNVVDQQEGNNKKPSPFFWENNKAQTIYELGCGRDMLELIKEFLLFFEMHYTNSIFSSESNLREDINRDQIAKKLNIDANDSTKPLLYFLLKNRQPEKKSEEKGQQQKIAQPSPKEVQIQQQQQQQQKLQEQKLQEQKRQDELKKQEELKKQEEQKKQDEQRKQDELRRQQQEEQRKIEEQKKDQLRKQQEEQKKMEELKKQQEQQQKQEQQRQEQQKQEQQKKEQEKLKEQQKQQELLKQQQEREKLEAEQRKAQAKKFEHRNYEDEKFDNEDLEEQLEGEDLRDSQLQQDQFQESDEYMQQSQSQGIDMTVDSVALEEFDYYEDIEDAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TR94</accession>
    <name>A0A8S1TR94_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T0240212</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DEAD box helicase family. DEAH subfamily. PRP16 sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2130:FF:000001">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000001">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000007">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000615">
        <property type="entry name" value="pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH7"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF91">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR ATP-DEPENDENT RNA HELICASE PRP16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="327"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="726"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="911"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="1148"/>
            <end position="1311"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="1333"/>
            <end position="1507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="1767"/>
            <end position="1805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="24"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="46"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="64"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8153426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1880" mass="215377" checksum="86688B74E80002CB" modified="2022-10-12" version="1">MDRESRSRDDSKGRQAPLYRESLYRSSSSVSSQFKVSSGPMLFKGTENSSVLKNAQPQFEEDADGFKKPPARSEKLEEERKRNKLLNEYTAFEWENIEQESDRKWYDYDEDDIGNASQQDWGGEVFKGQKEEFKFEEYSKRKGQTVRQSEKNQEQNRWELNRMIASDVFKRKADAWDFYEEENEKRVVIHVHDIKPPFLDGKVVYTTQLTQVQIVKDPNSDMAKLAKQGSEVLMLMREKQDKTKMRERFWELSGSKMGKVMNLDRKKEQDPDRHLLNEDGDYDFKASSRYQTALQRVTQGQSDFARNKTIKEQREYLPVFHCRSELVQLLHDNRVSIIVGETGSGKTTQLTQYLYEEGYTNTGVIGCTQPRRVAAVSVAKRVAEEMGVELGSKVGYAIRFEDYTSKDTVIKYMTDGVLLRESLQDPDLEKYSAVIMDEAHERSLNTDVLFGILKKVAQRRRDIRIIITSATMNAKKFSDFFGGVPIYKIPGRTFPVDVRFEKAPAQDYVRSAIKKTIEVHIQQPPGDVLIFMTGQEDIETTCYLLAEELNKLKQNRWELNRMIASDVFKRKADAWDFYEEENEKRVVIHVHDIKPPFLDGKVVYTTQLTQVQIVKDPNSDMAKLAKQGSEVLMLMREKQDKTKMRERFWELSGSKMGKVMNLDRKKEQDPDRHLLNEDGDYDFKASSRYQTALQRVTQGQSDFARNKTIKEQREYLPVFHCRSELVQLLHDNRVSIIVGETGSGKTTQLTQYLYEEGYTNTGVIGCTQPRRVAAVSVAKRVAEEMGVELGSKVGYAIRFEDYTSKDTVIKYMTDGVLLRESLQDPDLEKYSAVIMDEAHERSLNTDVLFGILKKVAQRRRDIRIIITSATMNAKKFSDFFGGVPIYKIPGRTFPVDVRFEKAPAQDYVRSAIKKTIEVHIQQPPGDVLIFMTGQEDIETTCYLLAEELNKLSEATPPLLILPIYSQLRSEEQARIFEKSEFRKCIVATNIAETSLTLDGVKYVIDTGYCKMKVYNPRIGMDALQVTPISQANADQRKGRAGRTGPGICFRLYSSLNYRQDMLENNIPEIQRTNLANVVLLLKSLNINNLLDFDFMDPPPQDTILNAMYQLWVLGALDNVXGQSDFARNKTIKEQREYLPVFHCRSELVQLLHDNRVSIIVGETGSGKTTQLTQYLYEEGYTNTGVIGCTQPRRVAAVSVAKRVAEEMGVELGSKVGYAIRFEDYTSKDTVIKYMTDGVLLRESLQDPDLEKYSAVIMDEAHERSLNTDVLFGILKKVAQRRRDIRIIITSATMNAKKFSDFFGGVPIYKIPGRTFPVDVRFEKAPAQDYVRSAIKKTIEVHIQQPPGDVLIFMTGQEDIETTCYLLAEELNKLSEATPPLLILPIYSQLRSEEQARIFEKSEFRKCIVATNIAETSLTLDGVKYVIDTGYCKMKVYNPRIGMDALQVTPISQANADQRKGRAGRTGPGICFRLYSSLNYRQDMLENNIPEIQRTNLANVVLLLKSLNINNLLDFDFMDPPPQDTILNAMYQLWVLGALDNVGELTELGRKMSEFPLDPPLSKMLIKGDQLGCTEEILTVVSMLSVPGIFYRPKDREAESDAAREKLFVGESDHLTMLNVFEQWKRHEFSPEWCNDHFVQAKSMRKVREVRAQLKDIAGKLGLKMSTCNFSYDVVRKAICSAYFQNAAKIKGVGDYINLRTGMPCKLHPSSALYSLGYAPDYVVYHELVMTSKEYMHCVSAVDPQWLAEMGPMFFSIKEDGETRASRIESEKKSKREIELSIEKAKREHEMKSEECQRRIEKEDRERRLISERTATLGCRPSRIQTPLRQQISSIAATPIRSECESVISGMTSIQAKQMRMAYYDDEEEQDVGRNKIPKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TT20</accession>
    <name>A0A8S1TT20_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0260100</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8154702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="409374at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="997"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1030"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1094"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1186"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1216"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1295"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1326"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1359"/>
            <end position="1379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8154702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1401" mass="162460" checksum="1B3752582D31EADE" modified="2022-10-12" version="1">MITFALFLIVCQAEYVKQILRDLSDLSEFTPIPQTYSCSVLNYDMVILDQKNPSLSLNTSIEKPHYFIHLYIDYMFLGQWQGESIQLLLNNNIIDEYTYIWTDKSNQGFICNGTSPQKYMTREIEFYHIDQYLELKFNMQTIDFGAINPMKIPEFPPKLGIRNIQVNAQECHPQCSSCSGPTENDCRACPQLAKKGKISNTCKCPISIPYIYADNCIAYCPKNTFLNYYKVCFGSSLIKNIETSLLNSFEHGQNNKSSLWSISMTRTTKNQLQFRHKDSYLIGMFEERQILTINQNSLVGHHRIRVRFNLYLFYTWAVNEYIITIIDNVRQKPLLYSKDGFINYANKDSCTNTEINECQIFLVDYYLNHTSDNLKIQIYSDTYKQSTSRWAISDFIVDVLRCTENCGNCLNDQECTKCETGYFLLKNQCVATCPLSYSFLQDTNCIEMDDETQQSEYLLKELVQTKLHGTVPNITSYFNGYRHLGGLQVSSTRYYQKFQYLKPHYQIRAAFKVDLLNIAQSQGDVYINLGNYTQTISTDPVRLYQTTTVNFTFAHSSKYLKLSIFSSSSSSNFFKSGISNMRIVIDYCYPTCTACTGPGIDECLQWSYDPNYDRQSYSCQLSTYFFNDSCTPCDSQCYSCNKQNYCTLCSSDFTQSGQSCYCSKGYYLDEYNNCQKCYEKCSGCVGPLQDDCFDLSSKEIVCDISKGYQIGQECNDGNYNIRDGCSNCIMDEGWSCLNEIGKYSICIKCPANCMDCKRNQNQLQCLKCQPGYFFYQNGCFQCKSECLECNYIDQCTKCSVPTISPLNGKCQFCETERGYYEVNGKCQAKCGDYRQTLEEQCDDGNLIDGDGCSSKCFIETGFTCANGICQFIQQQNKLELKYSNKTTTNTLKLDFGEYPAKCDEKYIKVYLENFDPKDFKVFLNLKEEGSMKYCQISFKFYRNVRDYDVIHVIFYKNKQRLLSVDFEEMIIQARSETFYTDEEQEQAQQVQQLQSNFVSALYFLGPGTILLGGFNFFFSILDILAWLNNFYYFNVMYPANVNIIFLNPAWELMNPFDFTPIVRMETDQDYIESPFRFEQKGVDPYFINNMMMCFVTWLLCASLYPICKCVVFIIEKIYQRSPKQKVRTVKIKQMPIFHLDQNLESQQPETEIKLEQPKQFPSAIQYIYDNAYQYQISFKAIIIKQLNLVYLDLCMASVLQLQFMDKSDQPLIFSQIFLSFIGLFICMSILLIAIKVSKQSSVLLNTKSYQFDYSTLYEGIDVKSDISKMYFSISFARKMLFIVFLVGLYHYPLFQTSFCCAASFLNLMLLIYKNPFESKVDYIQNAVPDATIFFVLVLCVVLAFDDKEQKYSSKTRTNIGWAMLSFISLSVLTQLGLILRQFIIDIKDNYQWIKNKLCPSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TW37</accession>
    <name>A0A8S1TW37_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0270227</name>
        <name evidence="2" type="ORF">PPENT_87.1.T0270228</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TW37"/>
    <dbReference type="OrthoDB" id="303641at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8155657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8155659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="221" mass="26725" checksum="1A019F90AA2DB7FF" modified="2022-10-12" version="1">MNTEQQECMPIKKFLLKKQISERLFNSHIHSPQHDQQRGSPQHQRKIIFPQIQVPQENTDQKHLISPKYQQSVEKMEYIKLPEIDEKFYIKRSIQQELCFNVKMQHSSRKNSTENNEIVDNDQFILPHHQNNKKVEFQQDVLVYDYINQTYKKSNIDGSQKPLFRRQKTKNLSDDLRLYNDNFKYNGKSQFFEISTKEKHNQKHFIIDTLQMENENQINKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U094</accession>
    <name>A0A8S1U094_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase II subunit beta</fullName>
            <shortName evidence="2">CK II beta</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0300104</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the casein kinase 2 subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000030">
        <property type="protein sequence ID" value="CAD8157343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U094"/>
    <dbReference type="OrthoDB" id="3971593at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019887">
        <property type="term" value="F:protein kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1820.10:FF:000012">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.20:FF:000001">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000704">
        <property type="entry name" value="Casein_kinase_II_reg-sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740:SF0">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01214">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01085">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01101">
        <property type="entry name" value="CK2_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="226"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="226"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="250"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8157343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="277" mass="32542" checksum="54A1D6EDBE6CBFE8" modified="2022-10-12" version="1">MSQSNISVNENGWIKWYCNLEDHHFFCEIDEFFIADQFNLYGLKQLFDHFEEALQMILSPNTPTDEDLEDDQFLELYNEASELYGLLHARFIITPQGLTLMKSKYLQGRFGVCPRVLCDRQNVLPIGLSHDLRTSRVKIFCPRCQDVYSPKKQMSDVDGSFFGSVFPHIFLSVFSELNPTQPANEYVSKIFGFKVHKKKGSKFQIQQNQTEMYYYAEDHIKRLSQIQTSQQQQQQQSQQQQQQSQQKQSQKNDDEAEKKQKEALNAIQKLQKQKQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U0I7</accession>
    <name>A0A8S1U0I7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0290210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U0I7"/>
    <dbReference type="OrthoDB" id="307334at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8156859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="487" mass="58461" checksum="9CECA16B4375997A" modified="2022-10-12" version="1">MKYIKNLILTFENKKLEQQYQIEKKGSIQQPIFIFIIVTSFISNLVVLGFHFFDHQIETWYLNIAFSILSILLFVLVVIMKKIKYLQDALTIVNITLGFLELNVDPATTEKLEFYSYGNTFMQLQAVVYIVSNFGHAFIQVFCHLICRFVLTILLSNRTDYLLVFISIISSFVILVTIYYNEKNSRKYFIQNLKENYWENNTSFIIEQPYIRFNFRKEQLLFNLIKCHKIECFPGYDNFYCEGCNIRKLLRTYFIEKNISVEDLLLQYKTELPQKFFISYKKRKYLLKICNVDIQQVQYLIILDKINNTTSQQEEKKEIQQANFIKFLRSNKKHIYQHFFNWGAQSLLFLNKKIIKKINLQELMIKLLKVFRIYIFQKISIKIITQDQNTSIFSFYYQTKIFMMQLFEILSTMQRTNQLQSQIILKRKGCDILIQITNIDQQSFMKQFESNFFLQNLSSLLLHNVEMQQSIQLHFRNYPIKSFAFNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U103</accession>
    <name>A0A8S1U103_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0310223</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U103"/>
    <dbReference type="OrthoDB" id="302665at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8158208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="234" mass="28119" checksum="26ABF5CAB4113A2F" modified="2022-10-12" version="1">MFKEYFYDVNEIESSQKIQKNAENLNKLLRNVGEKQILKEDCEYIWTRYHIQRFLNKHTIFKDEEVIEFKQLQELPQKKKQVRFEEEQAEIQQVNQFNILKDRVRTGGIDKEKRDRERAEMELKLFENIDKIKEISDIDSFKPLKYIVQFDDKGNIMLDNTELKDEDVEKLQGKQQQNISFEEFAEDDIAIKQVDLKKFNKKKSNSDSSEVQSSDSEDSEDDYLMQQRNLIKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U1E8</accession>
    <name>A0A8S1U1E8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0310362</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U1E8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8158480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="218" mass="26387" checksum="E9E0E5ECC6F1747B" modified="2022-10-12" version="1">MFWVYFVSIVLDGCVVLFLDIDNLYLNGHTEFLTSFMKSVTCIQNQAILWIKHEYLISTSQLLVIKKIRIRIPTYKHECSKYVFQLLENFKYRIFNNETYCFDGIYNLDRNQQNKVYIFIQFPPSDTQYIQTLLGQILQQLSYTNKHGLERRQIDFRMIQQQQWQTRRKLVETFKGYNKHDLPLFIQFCYKYLTEIPKIKLGSINIFLQLKLNNFNRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U1G0</accession>
    <name>A0A8S1U1G0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0310236</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U1G0"/>
    <dbReference type="OrthoDB" id="292748at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8158234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="164" mass="19114" checksum="EE2DF04073429BEB" modified="2022-10-12" version="1">MSVIRELSHQKRNVVLELFGTSKRRIEQKENVQSSINRPSKRFNYPQSSNASKLLLFQILKQTSSIKQPPYKSSKKNNESTGLNESICRKKILHIDIQEPKTYIKQVISPYRDFRSQVSFEIAPQTCVTVPRKISQPQQNNIQRNSVRRSNSFRDLFKSQITFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U1J2</accession>
    <name>A0A8S1U1J2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0320336</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8159135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U1J2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8159135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="190" mass="22506" checksum="42ADA2D82DB361BE" modified="2022-10-12" version="1">MQLIQNSKGDHIQQIQIASLIGTARNASISVHDEIEQLKYSKNSEFQVVIVNIILAYVEILFIVLVFKLYKFICSQSTILYENKQFKENYSSLQNQNQKSFNQKLLHCQLDSQSIVYFFTVYFYELPLLQTSFFCQSYFSNLALIFQEIPIETDIYLHSSSLPMHIHNIHYVSYIQIFEEKYIIDWIILF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UFL2</accession>
    <name>A0A8S1UFL2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0380313</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UFL2"/>
    <dbReference type="OrthoDB" id="93876at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042234">
        <property type="entry name" value="WDFY1/WDFY2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46189">
        <property type="entry name" value="LD41958P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46189:SF1">
        <property type="entry name" value="LD41958P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="PX" evidence="2">
        <location>
            <begin position="3"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="400"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8162927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="448" mass="53060" checksum="5841496CB462044D" modified="2022-10-12" version="1">MSLSNNTHIKIQVDSYTSEKSVIRYVIAVECSNNKWQIYKRYSEFEEIHFKLVELFNELPQFPKKQIFHLNRSEVQFRMQQLNDYLNQLLDRKEIVNSVIFRDFLILDQYDEFLHPVQTQIRIFNMCIKDIYENDHITILLLYDSSILTRMDTYMNNILDSKKQSPTSTLVCFKQNDFKQKLFCKEFFKALTCMNFNNTENSFVLGLSSGVVYYYRLDENYTIDMEEQFQIAQTKISGCVLVEREIYVITSNFLMIQNTKKIQELQEIPIGQHDLNNIVYNSIRNLIVISNNIGEIYICEVPFVKIGLKVQTHTIQIKQILIDNERNYLMALNYDRSHIIIFDFAKGLQNGFTQIKTNVEIKNKSLCFEWSKKRGEIFIGNEDGTISIWNVQDLQPFYVFKAHSKGVNKIIWNENQSLLMSGSDDESLKQWYFPKKWRVSQADNFSTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UFP5</accession>
    <name>A0A8S1UFP5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0400030</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8163728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UFP5"/>
    <dbReference type="OrthoDB" id="190265at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000284">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035728167" evidence="3">
        <location>
            <begin position="17"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="5">
        <location>
            <begin position="42"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="4">
        <location>
            <begin position="144"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8163728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="357" mass="40645" checksum="82D1113E660CE154" modified="2022-10-12" version="1">MKRIVLGLLCIGICLSYNRNVQDTLIAEMENEHVNEHDQLIFRNWMLEYGKSYDNEFTAIHRMQIFMRNKKNIEKHNHVGAKYKAKLNEFSDQDYDELSLKMFMHLDFSDDDIKFQNHHFFSKEDIKELRNHPILTQMREQAKKGDSLDWSKQVTPSRPQGTCGSCWAFSSSDVAISRLALKGKEELTQLSKTHLIDCCVGDKNKGCNGGSPIGAYKFINENGALRENEYREYDATQQDCTKPGGNIGKGLIVDVQKVDDPNFEQIKEALQDGPVTALMYADKSWFEYGGGIIDTCSYVGAKELSHAVVIVGYGKDHYKVKNSWGPGWGENGFFQVQRSGAPECLDKIKKISYPIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UH26</accession>
    <name>A0A8S1UH26_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L36</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0370133</name>
        <name evidence="2" type="ORF">PPENT_87.1.T0840196</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8161966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8185150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UH26"/>
    <dbReference type="OrthoDB" id="302801at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1760:FF:000009">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000509">
        <property type="entry name" value="Ribosomal_eL36"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10114">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01158">
        <property type="entry name" value="Ribosomal_L36e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8161966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8185150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="110" mass="12622" checksum="6F56D841E0ED57B3" modified="2022-10-12" version="1">MGRTGIAIGLNRGFITSSLTKKQLRRRPSQRKGTLGKRVLSVRKVIQEVAGLSPYEKRIIELFKTNQPKDLKKAGKLAAKRLGTNRRGKRKIELVQNLYRAMRKQQQTKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1URJ6</accession>
    <name>A0A8S1URJ6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0440125</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="354346at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009190">
        <property type="term" value="P:cyclic nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627:SF12">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="8">
        <location>
            <begin position="71"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8166392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="190" mass="22169" checksum="C308B560D3C0982F" modified="2022-10-12" version="1">MAAAKTRGSDTKSSRFQKNRKIYRRYRQSKGYCYHSLLFCLLVQIIYIIFHQDITRHNLSXRQVKSIIGFDGLDMRIGIHTGKVIGGILGTEIVRYDVYGADVMISNKMESNGERGKVQVSEDTKQLLEQSYPDQFLFTFNKEVEFKSISRKTKGYFIDPVKNDSNEDFDDMNHFQFVKDLEHYENNGQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1USF9</accession>
    <name>A0A8S1USF9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Apoptosis regulatory protein Siva</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0420182</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1USF9"/>
    <dbReference type="OrthoDB" id="295998at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022773">
        <property type="entry name" value="Siva"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05458">
        <property type="entry name" value="Siva"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8165376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="134" mass="15556" checksum="CE939F0B6DCDA039" modified="2022-10-12" version="1">MFRLSPYYTNVRQEVYSEINYQLAKELDQMQQYKDQTGSNSKKSASKQNFNSIFYKRTNDQESIIEDQIIKKCSNCEIKIVSDDIYCSVCTKLMCQKCINECSACGNNCCLICSKYAYLQSGDFLMCLQCIQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UTY9</accession>
    <name>A0A8S1UTY9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0490187</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UTY9"/>
    <dbReference type="OrthoDB" id="10252009at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF519">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE MPK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="6">
        <location>
            <begin position="19"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="7">
        <location>
            <begin position="78"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8168580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="159" mass="18408" checksum="3A00137ECADF4AB6" modified="2022-10-12" version="1">MQQSQAINLIPESTSLIIEPTDTTGALLLGNISSLTKSYKQNQVKTAITVCEFSKFDDIHLDNHLVINIEDREDENILQYFEKTNKFIEENLKKGNLLVHCMGGISRSATIIIAYIMWSQKRSYKDAYKFVDEMREGIYPNEGFKHQLKEYELQLNKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UYJ3</accession>
    <name>A0A8S1UYJ3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0510222</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000051">
        <property type="protein sequence ID" value="CAD8169735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UYJ3"/>
    <dbReference type="OrthoDB" id="304205at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001129">
        <property type="entry name" value="ras-related protein Rab-44 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8169735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="297" mass="33818" checksum="B103D4AD3F32856B" modified="2022-10-12" version="1">MKQSFQIKSEIQLCFKIVFLGSSSVGKSSLIKRFLRNEFAMKSMSTVGVACESKIVIINNQQVKVQMWDTAGQERFRSLTKNYYRGCDAVVIVYDVTNMKSFDQINNWIADFDDKCEKPAIKMLLGNKIDMQTRDVSIELGIAYSKRKKIIFEEVSAKDNINVENAIMRLIGILVSQTKIESGDKNLRRGSMAETKQTIIEDPFINKSARLYKDASISDHSKLQAIKSADESLQEDIQINNTSNHQLTSSQDNHNNMNLNSQQPTNNIKLIHPRQSGIIRDEYIRKHSSNQNCMCSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UZC3</accession>
    <name>A0A8S1UZC3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0520110</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000052">
        <property type="protein sequence ID" value="CAD8170041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UZC3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="195"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="88"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="195"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="209"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="225"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8170041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="235" mass="27755" checksum="E2F6C4C55FD6617A" modified="2022-10-12" version="1">MFQLNNNEKQIDFRKYQMEQIDHIYLSFSPKQEEQNTLNLKTEEMSIPNIQSFFTDRTSIFDQKVGYFQGQVHNSDQFDFYLISKTELKKLLKQLDSLQKIKQNYDALNQQIQSTQKNPSKQLQQHSYNNLQKSGTTLQGSDQKARNLQQSQIMNEGQPHKDVISVRALVGHRDNSIHYNNNNKENNQGLINNQRTTSQQKQQNHQQNYSSVRKLNQPLPSRQRSQTHIHMKYIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V330</accession>
    <name>A0A8S1V330_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0540055</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8170987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V330"/>
    <dbReference type="OrthoDB" id="550424at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000105">
        <property type="entry name" value="AT19485p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000357">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051339">
        <property type="entry name" value="DnaJ_subfamily_B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078:SF553">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="2"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8170987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="327" mass="37238" checksum="296F3228A147BF3D" modified="2022-10-12" version="1">MDYYKILEINRDASASDVAKAYNKLSLKWHPKLSKLDHNTTYHHFCLISEAYEVLSDPIKRTFYDKYGEEKLKEGFFANGNLKGGYSFAGNPEEIFEKFFGTSNPFAQLIDTNGSENHGTLFSHAFGGQNFPGIPGPQDLEIQVECTLHELYNGCAKTVSYQRQILNKDGITTRQITETKEIKIDRGIETGQKIVYKELGNEAAGFKSSDLIFLIKETPHPTFKRKGNDLLYIAKVKLANAIAADPIQIVTLDNRKLQVPVDQIISPKYVKMIENEGMPIFQQDEVKDFGKPYTFGNLYIRFDIQFPEDLTESQKNRIKDILLEGQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V3E8</accession>
    <name>A0A8S1V3E8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0530006</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V3E8"/>
    <dbReference type="OrthoDB" id="295954at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000616">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="16"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="298"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="362"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="428"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="298"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="318"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="385"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="440"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8170332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="515" mass="59455" checksum="C6B303A6D452AA34" modified="2022-10-12" version="1">MSIQNQQLQRLINNTYITKKRVSAGSFGVVYCAQDINTKAIVAIKIDKGNKEDTSLEREAEILKRLQKTPQIPKLYWAGKDGDSNVLVIQYLGRDLTHFMKTFRKFSLKCVLNIAEQMINIIENIHKNKVLHRDIKPENVLVGKEDEDNLLYIVDFGISKFYKDENDSHISFRENQPFIGTTRYASINAHKGFSLSRRDDLESLAYMLIFLLKGQLPWQNLQFIDEEDKMRQVGQMKMKIDMNELCKGIPIEFGRFLDYIKGLPFKAEPNYKYCQSLFKKLSQEHNYQQKDLIFDWDLGPKSDRQDDKKKQTIEGHMPSSSNNSNLINNKPSLFKKSKDDISSNNIGGSLLNYSQEQLEMNSLLKTPEQRKSSLAPDINRRKSRMQSVSSYNDSHSEIDFNNGSVLLGIQPSMLSRLSKISFNSNSRVKDSKQNLSLTPEQKRSNLHEKKLSITPNSYLQQITGQKQNKIVRNSNSQQKLSYKKEEETIKEFSKMNEADEGIETSVNARFKKSIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V3U9</accession>
    <name>A0A8S1V3U9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0550094</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V3U9"/>
    <dbReference type="OrthoDB" id="303416at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="226"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8171575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="552" mass="63849" checksum="837CE5938C6A1F27" modified="2022-10-12" version="1">MKSLLQRSSYGELSSIRSYPTEKLNTQSDSELVEVDMEKLNFHSFYLLSDPIQLQSNQVEGELLIKDLIKKIIAKVSKKITENEQIKMLPIIGYQSSIDRMSHPIQQYFRQHEDPTFDYLTGGLEEDEEPVRFQQEQWLVQKVNSIPAKILEVEQQSTNSNRQSLTAQTFKRSRVLIQDQAQLNNQKNVREKEKLPGIVDLNDPLDISMTEELLRAQKERDYKIKQDLKEQEKRRKQKEMEEQQLKYELISKDKKSKSYTYDYDGKLISVTALKGHKFPPTAATLGSKFEDGQTKINMFNKRKTFFQSSSSQIKKKQDDEKEKDPKELIKFQSLAPLVNENMHLQSGVLIIQEGRVREGPKDQPINLDKMSDPALRMIRNEYLSLTQQINASLNQSSAQLVKAEEAKIKEFSNDQDKKQILNRIRRLSQPSITSDGNIKINSMHMYEQVAQVIFENKEQHLIPINKPINNNNTKDMQIMKTPTDQYNISLYKNTALSKEVPSIQGNTTKLKGSKAKVKDMLSSVGIVVKRTRERRGNSEITHLPKMKNISQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V3W8</accession>
    <name>A0A8S1V3W8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0530101</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V3W8"/>
    <dbReference type="OrthoDB" id="290402at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8170522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="163" mass="18961" checksum="09DC31D14715F0D7" modified="2022-10-12" version="1">MLISSAKHSVRPSTNESNSLNTRLCKNLQSMTLSARFQTELQQLESQNEPFLFNQQRSTLSSVDMSRQISSKQSYRSSMPNINNNNNGYHNLSSKRKSEGLILRTTPCLIYMPGLKQEEFKEETQQQPVQKKQFKAPLLKTPIKHISFQFRNRDKWNPLQKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V5M5</accession>
    <name>A0A8S1V5M5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0560067</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000056">
        <property type="protein sequence ID" value="CAD8172064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V5M5"/>
    <dbReference type="OrthoDB" id="304545at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="568"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="796"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="288"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="599"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="570"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="808"/>
            <end position="827"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8172064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="972" mass="113814" checksum="F4DCFDA56F6F3985" modified="2022-10-12" version="1">MEEQVKKLQIIVDHLQKRSKQPNSKILMDCYEVFSECILIVQREINLEIGSIMDKLFMIIREMFENSQLKIQPPIEPQQIIVATKIPEFVNALQLGSVSVLDQLGNVEQVKNIHQIPWCDHKCKEQKPRNFKGFQDVGTSTLIDYYENCQKSSSQILKNEEEIREWIEFTNDTIAMLGEAIANKKQLDIEYQGKPGDVDLLISDIQFRRLNLEQVLKSVNPENFGVQNFDHTVQFLQQHILQIQQQTAIAVMQKMKHRQEEKDKQQQIINDMKGKIKKKVMHKEDCRCAQYVNDILSLKNKLEQIQTDLAKKEHYILELEIKNDQKDIQYRMLQQQFYQMESERTEMKQKLDNYTRFAKKNSDRGYVSQSSNDEIVVNGITAHPRTTSRDQSQQQSSKQQILLTPKENYIIQKKTQQMERLVKLVNALDVNNQTRRLTQMQFILEQKVTSQINDLADLIKDIEENDLLQNDVIDNSYVTKEIEPISSRFLDKQSKQYTTTQRNSLQNSFEIEMNIDKPIIRVSIPVEQIPYQRQVPSNRIMSSVGGSQKKQDLSNINYNQRQMDTLKIKNSSSNQLSPNSSNVRMKSSQNISINRQNSIKNQQQQQQQQQQQQQQQQQQQQQQQQQSQIILDKSKKQKNKKNTKDQYQQTDNLIKIEISTQTEKILNRQTTEQIDNIRIMGSYNKNNSQQLTTSSTFHFNSLGTTGNIITPIQSNNNSNVQSPRNQISHQHLNSTITGSFPLPSPSSTLQKNKLQSVKSESENSQLLGRQLSLHTIDNQSSKQSQSLKQRILDRQEEKHQKNNHRKIYTSTATQFQSMSPRENSSMKLKSNDDMRLQQFQQNQEEEQLMYDQVLHDLPEQHEHEIIQKAINRTPGYELASELNENILLTMFDNLQNVPEFIELIKKLNKNNPVTFNEFKRFINRIQAFHKKCGRECNHLQRFYLRLGFVSTKYLNKRKQLILSKPKVLPGFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VDS3</accession>
    <name>A0A8S1VDS3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0610258</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VDS3"/>
    <dbReference type="OrthoDB" id="312927at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013694">
        <property type="entry name" value="VIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45737">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45737:SF6">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08487">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13768">
        <property type="entry name" value="VWA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51468">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VIT" evidence="2">
        <location>
            <begin position="31"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="1">
        <location>
            <begin position="349"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8174741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="856" mass="99544" checksum="56B9CBBA216BB0F4" modified="2022-10-12" version="1">MITNTLFKPKLESKYFNVSNKVRQTCCFCPQSLIICYDLIRKIPIPLKGVQYFLKVQPGFVIVELKQTYSTTGYNQPIELEYLFSINNNAAVTKMIVELGDTKVYGIVKEKEEAKQQYEDGINQGKTMAYSEQDEEFPEIKRVKIGALAPDKQLNITFEYIQSLDIVLNKLWKVEVQPMIDNNYFSLNQQNQRVYNNYFERLNRYLEKFVKIDQFEYNFKQDITVSIDMGSPITYYKCPTHKILSGNAKNEQTKQEIGEEKLKKLHLMLEDTPSNFIPTKQFTLLFSSDDINLPRSILSHTNNDALNTQKYCATLTFIPKFNQTTLDDAYSQYLDGLNIAGNQVINRGNYLFFIDRSGSMSNVRITRAKQSLILFLKSLPEDCRFNIISFGTQFISLWSDSKEYSQSTLEEAISHVNSMEADMGGTEIFKPLQDIVYNEQYGKSKTTTLNIFLLTDGEVNSAPIIDLVKRNNQAETRVYTLGIGDGCSQYLIKQLAEVGNGKYQFVSDDEDLNAKVIDLLEDSLTPYLQGFNLESNITCINQIVPNPESVVCLKKNQELTIQVLFSVEQENENLQLTLSCFDPQEQKPIKYSVSLNLKNSQENEYFHKLASHKFISYYENSVKYGDNHLNFIKINKDTIDDQDIINTSITHQILSSKTAFVCEVCDLEDQFKQQMKQKVYIPQIQIIRYEEYFRYFGLQPVGCCLQKKVPKTKNSEKSIQRKSSRIQQQKITKEKRMQKQIQEDIDFEDELEKRQQLQTQIKKIEYEQLVSYSQSDGSFLYDGQIEKQINIEGRKNQQSYPQNVWLTLLALLYLDQFCLQYRKSWRLVYQKGIQFLQKNGVSYKQQKETVLNKQNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VJZ9</accession>
    <name>A0A8S1VJZ9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0670270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000067">
        <property type="protein sequence ID" value="CAD8177560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VJZ9"/>
    <dbReference type="OrthoDB" id="295282at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8177560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1119" mass="131568" checksum="A3D497133E4B9144" modified="2022-10-12" version="1">MSSKKLLYQEIDYTKQQLSLQMSHQKTTLKEFSSYLKDYQAILKSISEQLIKLTRKEFTCREFENKTIKEFIDKNIISTSKISDILGVLSENVNQIEQDIFQAYLDIKQNKELVMQKCDQQKKERDQLVSAKNVEKQQIDQFTQSAIIAMQNNIEAAIITEKKKNAKIIDSFKKFYIQQKEHAEIIGKQLMLQIQFFDTVQPEQQTLERMKNQLQAQSINQSGKKLNTSGTGQSKQNENGSINETDLLQSHKDKLIEIRKIYASNSNTKSLPIIIKDQVGIFNNEIPYNMDLAKEFQQQEFFQFTLLMMLRYKCQNQDWPLLITHTKNKFKINLASMKLLELFSNSIQSNIEQSVTSSIFITLIKDALMVIKTPQLYNEIKNNEKHYIILKKLLMFSTLNQYWVQCLLLQAFNIQNSQLDQQKKYFNNLLKQIIIDEKDIAQINFDSQIEEISYPLNQCLLELILSAIIQPTTIDMDFVPLIYQFASDALKLSPAHVSITLLSLVYQKILQPALRIKNLDIKDAVVITQQLTEQLWDIINKEKIINQSQLQFALKQKNSDRLYKDLLGLTTSFWSQLPSDQSFLIAFMNLFYIVASISLEFTEYQNTNELMLKIMEQTGHHYYSMIIKREQFQQNQISEKTFNQVSILLTVMCKECLELKEQYIDYFQKGIQHLLPDNRNNQNLFFFHFMIGALSEIYLILQQVELCIQKGLIPEDKNGNAFSICFTLALIDDQLLPNNLNAKALQIIGRSIEMWFQQSQQNTLKILQSLITKEKFLDESLQFKELMQQTQTKSIEDFCSILFKSLEALQQVVKFKNDNLFKKTINSKMEHILYTAILMYLEQMEQNLEQKTNLISYWFPAVIKSKQSIPLDQKQLAQQQIHLVIEMMMRICNLNFLQQQFQHLKDQLKLEALDHLTSDFNTKGKVICKSISKILINEIISPNLFTPLHQDLKSMTDAKSYQQKSEWSMLQQLDEIKDFFLIFNQKLPVNYHSVFYDSFIASFFEGLFYSIFDINKLQNISQDLYFELLTLEFQTLQSFLEKQISSNVLQMRIKQQLAEIKDISIWFSMQNKDLIATFEQYHLRQDNKKICSALYRLILLRKQDSKMKDFAKKYQKIYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VK79</accession>
    <name>A0A8S1VK79_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0680046</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000068">
        <property type="protein sequence ID" value="CAD8177714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VK79"/>
    <dbReference type="OrthoDB" id="289935at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035835002" evidence="2">
        <location>
            <begin position="16"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="52"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8177714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="364" mass="41293" checksum="ED5AAC04DCC5E26C" modified="2022-10-12" version="1">MKSTLIACLVLSVFCVEISLNNVATTQALEKLKESQWASFIVDFAEVELSSGGALTELVEAINQLIEQLEEELDDINHAYSKRTDEHNRDVTRLEQEIQDADRDIFNGEDFIDNVLIPQKERFQSALAQLKTNIEENRRVLDGETVNRKKQHEQFLSNIAEINEAIGAVDESLGLLSQIANPSLVQFKRVQTNLNRIQTSFQNHSSFAPIIRALLELATEQNFADQGSLQQLVKIFNELRVQFVDSLNQETADENAAETKFAERVTQLEKEFAEFQRAVMIKNSELAANEQKLGETIVYVGQRKDDRATLQAQLQAENDNYASETDLYNRTVTEYNKEIEISKQALGLLTQPSFEQYVKSKVGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VVS3</accession>
    <name>A0A8S1VVS3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0760147</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000076">
        <property type="protein sequence ID" value="CAD8181450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VVS3"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="3">
        <location>
            <begin position="35"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="519"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="376"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="519"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8181450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="538" mass="63510" checksum="568E4B6F1005FB41" modified="2022-10-12" version="1">MQNQFNEQELLYIIKNYSKLLSLFEDHNQKQTQVIILIGVTGAGKSTLFNFLSGAEFDMIGKENQQFLNIKDGAQQFAAIKGGMKSITKSPNYYFSEMYNALLIDFPGFQDTDGKIDQVLIQLLFYQICRSANVKIVYVVKHPETRFNNRGTQIQDFIRQTFQGQNLEMSQICLMLNCYNDKLSDDNLIQNVQGQLEQIYRCRPQNICVMRKIKKHQDIQQHFTIQKRDFLFQQFLKSNSVFIFPQYIPQSELISQYLSEKSEKILEQLSSTLEKSLDNQIENLNYELIKKVENSLKEISITINNQQKDMVQWFLELINLLIRSNELLNSENTTNLSYQNFLTIFNFFASFSEFISGFEDIRHNGIIAQEKCEIIIKIVQTKIDVLVKQRELLEEEEQKRIEAQERRRAEKNRQLEAQQAQQALQNFQKQSVLHCDIQNNLNKEIKETFEVKFLNQELIHKHQQLEYDNQRQKENKLNELRKIQQSTQISQSQLNLIDDEQGISQKKIDDLTKSCQEIQKKNNSEHEKKSFNQRNYSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W0D7</accession>
    <name>A0A8S1W0D7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0780088</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W0D7"/>
    <dbReference type="OrthoDB" id="10251154at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00195">
        <property type="entry name" value="UBCc_UEV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002609">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000171">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012952">
        <property type="entry name" value="BING4_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040315">
        <property type="entry name" value="WDR46/Utp7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14085:SF3">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14085">
        <property type="entry name" value="WD-REPEAT PROTEIN BING4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08149">
        <property type="entry name" value="BING4CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01033">
        <property type="entry name" value="BING4CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="228"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="UBC core" evidence="6">
        <location>
            <begin position="523"/>
            <end position="676"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8182227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="676" mass="78252" checksum="22BE3903260032D8" modified="2022-10-12" version="1">MAKDKILSAKLKQQEFKTKSTQLKTQKIAQLLEPQFQQSVEIDDERPLLKISNFKDYLPEENVQNIINLKLKSGPYKLSVTPQTTYLAGESGHIAAFNWRHKQLECEFDVKEKCYDIVSIAQRNFAVAQKSCIYIYDPKGVEVHKIRECKQATKLEYLPYHYLLAALNQNGQLTYQDITQGKIISTYRTTPSPLCLKQNNNNAILGIGDQMGVVRMYAPNTGTSLTSMLCHKGGVMSMTFSRDGNHLITSGSEGTIKVWDLRTQKLQSQIGVNATNLALSDKGVLAAGRGTDIIMWKNCLIGDLKTPYLRYKASSMLCDIDFIKHEDYLAMSTFDSYEQTVVPESGEPFFDTYEQPELQNKKQRLETNVRQLIEKLPPESISLQSYRIGTIDRTSKSIIEKEQKKQIEEQASKKIKKQKKKMRGRNKIGKREKLKELERDQKQQVKWQEMLSDALRVKRAEKEKKKIETKLLDRLEQMPSKEFIRMIAFQLKDVDKGLMPDRIIQEIISRLPKESVEKAKYFSQLFDQASQILTDEGNDEFVKIEKAIINNDLRNQVWEVNFRGDENTEYQDKKIQGYLILPNDYPTAAPKFYFWFFRDNSKIIHENIYGDGLLCMPMVTNNWKGNEGLTQVLMTIRDIFNHPNDKDPANPIFTKAPIIEKTRRRKTQSELLEDWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W1T8</accession>
    <name>A0A8S1W1T8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0780024</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W1T8"/>
    <dbReference type="OrthoDB" id="436852at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46364">
        <property type="entry name" value="OS08G0421900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="5">
        <location>
            <begin position="167"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8182099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="228" mass="27367" checksum="3542DC410BF2C0A2" modified="2022-10-12" version="1">MNDLNIYNILNYENYDQLVQLFNENGACQFYSSIFLHSLDITLYKEEPIKYLNKKNQNQFGIIKEIVCLNLKNKNQLPLIKIQVLLTTQFVSQYVNTKIADWLESRELFSCQDTQWICWSDIQGKIILVKHDEIPSYANKKQMVYFMRASFNHYTKQFNPPYDQWQRQYCVCGNPDNHEKRYVQCDICDIWYHMECEGLTQQQCDRLDKNKRLTYSCNSCKIGKKKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W4S0</accession>
    <name>A0A8S1W4S0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0810146</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000081">
        <property type="protein sequence ID" value="CAD8183727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W4S0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8183727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="196" mass="22319" checksum="3B8AC8E6CDDA3BC6" modified="2022-10-12" version="1">MKSQNSKKPNLKLTLNKKSMGQLIQCDKPPSSKCSQKTTSSNGKTSNSSKQLCFKFQDNQSTANPSLISEGDKKLLEPDQSQLIQLLIQENLQLQKQNSDKDSIISRLITNTNKKPVILQRANTSNTERTPEKLFTIKKLQQNTCEKKSNSPLGFTFCNVDKEDEGKVIQSQPMTRSQKRLPKEFQIVPNQRRFFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WAA1</accession>
    <name>A0A8S1WAA1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0860035</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000086">
        <property type="protein sequence ID" value="CAD8185867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WAA1"/>
    <dbReference type="OrthoDB" id="296044at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:002129">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="134"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="426"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="56"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="20"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="58"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8185867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="556" mass="64634" checksum="04C6D27BCEB10B77" modified="2022-10-12" version="1">MGVCTSQSQPKKVKQHKHQSVTMPPQQPLTTREQKTVTVQFQEPTSIQRITTPVRRVSPNQATTPVKKASPNQATTPVQRVSPSLNSTPKTMIVQSTSQQQEKTRNGSLLNIPMQRMSTKPGTKSIQSPASRKYSVVSGQNHNNHSKTVMQHNETGQLVLVEMLKFENRNQQYIDMLDELQLNHMHIVKIVDIHIDSHKRNYQVVYEYCQGGTLSKFLESNKLEYQTIGSIFYQMVEAIAYIHQLGYSHDELTIDSFSIFDDTSTPFIKLSEIRSIYQFMYPKNSLNYEPPNSPNHNHQKDTGKFQNIQTKNRSQSNDVWALAIIVLQLLNQQFPFDIEEINKFKPEKVYAKQQLDIYPLIIDMLRHNPNERLTLDKCLLHPYLMNMKQIQPKDYLQPFINITKSKNMTYLHKCLFQYLLSIYATDHLKVLTKLFTNADLDKDSKLNEQELQILFNEKPEEYSFNISDFVDITLEDFLLLAADKQFVLTQDCLTQSFKTLSKPYNYLTPKLICKVITDCDEIKLQQDFDAFNLNYSFQQQEYEGFLINYQSPKPIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WBB6</accession>
    <name>A0A8S1WBB6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Beta-lactamase class A catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0850255</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WBB6"/>
    <dbReference type="OrthoDB" id="292897at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008800">
        <property type="term" value="F:beta-lactamase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030655">
        <property type="term" value="P:beta-lactam antibiotic catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045155">
        <property type="entry name" value="Beta-lactam_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000871">
        <property type="entry name" value="Beta-lactam_class-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35333">
        <property type="entry name" value="BETA-LACTAMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35333:SF3">
        <property type="entry name" value="BETA-LACTAMASE-TYPE TRANSPEPTIDASE FOLD CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13354">
        <property type="entry name" value="Beta-lactamase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035863639" description="Beta-lactamase class A catalytic domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-lactamase class A catalytic" evidence="2">
        <location>
            <begin position="148"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8185755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="481" mass="55377" checksum="DF86EF14ED94202F" modified="2022-10-12" version="1">MFRGSHFIFLSCILLAIVSCEFIPTDEANFALFNVIKQAYNSDNFALIYQQLDDEVQQALSLKQFEEILVTKKSKTGSIEYWSYSSPIPQHPIFILGFQKNLAVGSIEWEYQETKFKVFLLKEFVEPTFVEFLKRNPSRTSLSAKTDDIEILNYQGNRQQDLMSVFKITVAIEFSRQSAEGIIDPDTWVSLEDVNKYYLKDVDTSHTQFLEIWNQMGKIKDDKIQLIEIANGMIALSSNTNTEYLQTILTMESIEKTIQTMKLEQTQSYYLSSLFLVFLNYDNKDRDDYIKQISSFTPEYVLEKSNQIHSILEQGGQEAQDLLARGKEVLDGQVLGIQAQTFTKASTSAYVNLLDKLNKQEIFNSKFYDIFYPLIGYVSMKNPFLAKTYKHVGVKGGSSSYEKDTQCVLSIAQFQELYNPFPYSKTTYALFTQDLDYETEYAPLITQFNYFTGLISLNGYYLQAVSKALTSISLDENIFEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WC99</accession>
    <name>A0A8S1WC99_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0870074</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000087">
        <property type="protein sequence ID" value="CAD8186437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WC99"/>
    <dbReference type="OrthoDB" id="284207at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035897805" evidence="1">
        <location>
            <begin position="22"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8186437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="261" mass="30152" checksum="493CA0F29B1AF3B2" modified="2022-10-12" version="1">MTPFVLLCILVLGHSMKVSYSNQCTCSKFSSDTSCLEQTNCIWRSEGCQKRDCTEYYDKDKCNRVASCSWNVSKCETFTKCSNYFMNDSFDCYKIGDQNLNLYCEPSDEGNTCKEYKPKQCGDVEEDCTGFQSQWSLCYWNNNSCNLVDIRYCDQLFNEELCLIFGEGLGCQWKENKCTVISCLDYTSESTCVLQRQNFIEDDPLLCRWSEIKCEEAQDISHLDFSNCLVNSFYNYIWDSINKECVSCLTYVPPSTTTPQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WFN1</accession>
    <name>A0A8S1WFN1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0890100</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000089">
        <property type="protein sequence ID" value="CAD8187485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WFN1"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000334">
        <property type="entry name" value="Heat shock factor protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF206">
        <property type="entry name" value="HSF-TYPE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="3">
        <location>
            <begin position="25"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8187485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="384" mass="45741" checksum="626098FE3B23A71D" modified="2022-10-12" version="1">MQIDLLDQQIVKKKRSQSNKDNEDSIPAFLLKTYEIIENPQNNEIISWNEEGTAFIVKKVNEFSNIILPKSFKHSNFASFVRQLNMYDFHKTRHDNNENEFKHNLFQKGKKHFLSQIKRKTSDVKEQNSLSLIKNEQIRNGQSENSEVYSSQILMQMGKLQNKQMELEKLIKIFIKQNEKVMKENKYLWSELTKNKHKNENSEEQIMKWVLQSLQGTKQNNKLKSSIQSNNLLMLKQTSENDDEIQFEQQNQRNFENEKLIFDQLPSQIISQNLEQQIEKLSKTQIIQVLLSAITNNQKQEKKNNNTKFEDEISFDDEYPVNKKIDIKQENQDKKQENQLIVYNEPYFFHKLEDNYIISESPNPSRKNSYYDQDLIPQFLDSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WGV9</accession>
    <name>A0A8S1WGV9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0910035</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000091">
        <property type="protein sequence ID" value="CAD8188231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WGV9"/>
    <dbReference type="OrthoDB" id="290617at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="48"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="174"/>
            <end position="175"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="8">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8188231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="283" mass="33252" checksum="1961878E6CDB4E53" modified="2022-10-12" version="1">MLTNYTYFRMPTYENRQPYIINTKNKKNKSVPLPKTPRREYSQISSRFQKIKFLGKGKYSDVYMAVDRITNCIFAIKIIKKSIINQFNLQNLLTQEIKILSQMSHPNIMKFYGVISDDKELAIILECCESSLYKLYDKLDQINEEQKLQIIFEILQAFSYLHDKSILHRDVKPENIMMIQNHIKVGDFGLAIKLKIKEKMKSICGTPQYMAPEVFKGLEYDIKADCYSLGVIIEELKCKSNEIRNMVHIFKQNDCNLRGNVKDFLQLKIFSKVKQILSQTIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WGW0</accession>
    <name>A0A8S1WGW0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0900113</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8187925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WGW0"/>
    <dbReference type="OrthoDB" id="303422at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8187925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="213" mass="25566" checksum="AA995D1819E34139" modified="2022-10-12" version="1">MKLSNRSSQLTVFQSEIYSRFLDMHEELSKSSKNINNLINHSLRQLASMKEEARFSFSGNSLNQQELNSFEEMVIQEDLDLETQPSNNEKELILENKIKIYESSLIKFEQEYLQKDQEINRLKKLLNDQNLQQTMQNPDEIYQEQNVIRQKLQILDGQLDYLQEKKNFRKKMKEWENIIKEQSDIINSQKLEINRLKHINQYLHKSIEKLQTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WIG5</accession>
    <name>A0A8S1WIG5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Actin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0900145</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8187989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WIG5"/>
    <dbReference type="OrthoDB" id="286570at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000007">
        <property type="entry name" value="Actin like 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000218">
        <property type="entry name" value="actin, alpha sarcomeric/skeletal-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000291">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000058">
        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004001">
        <property type="entry name" value="Actin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00406">
        <property type="entry name" value="ACTINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00432">
        <property type="entry name" value="ACTINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8187989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="375" mass="41664" checksum="868333788A1E23E3" modified="2022-10-12" version="1">MSEEHPAVVIDNGSGQCKAGIAGDDAPRCCFPAIVGRPKHQGIMVGMDTKEAYVGDEAQARRGVLSLKYPIDNGIVNNWDDMERIWHHAFFNELRVSPEDHPALLTEAPMNPKVNREKMTQILFETFNVPSFYVQIQAVLSLYASGRTTGVVVDSGDGVTHTVPIFEGYSLPHAIQRIDLAGRACTQYLVSILNELGISFTSSAELEIARDLKERLCYVALDYDAEMKTYKDSAANNRPYELPDGNVVVIQSQRFRCPELLFKPNFIGLEVPGIHELTFKSIMKADIDVRKDLYGNVVMSGGTTMFPGIPERLSKELTNLAPSSMKVKVVAPPERKFSVWIGGSILSSLSTFQAMWVTRSEYDEAGPQIVHRKCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-06-18" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WNP1</accession>
    <name>A0A8S1WNP1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0970134</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000097">
        <property type="protein sequence ID" value="CAD8190822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="299458at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="NACHT" evidence="3">
        <location>
            <begin position="245"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="918"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="960"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1002"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1044"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1085"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1127"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1169"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1211"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1253"/>
            <end position="1294"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1375"/>
            <end position="1414"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1415"/>
            <end position="1456"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="113"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8190822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1538" mass="179173" checksum="BFBB5B88D91E293C" modified="2022-10-12" version="1">MNNKEKNNNESGSSDYKIRECLAFNIIKIQQIIQEEPINKFSQNIISQLWIHEKDERVKRVLKNKEMIELQRRLFSKDINTISETLKQTIQQKFDLINMIEQEMQMEIDMKKKDQLQKKLIIINQELEEYVEGINEMSSKMDLNFAFLKEIQKQLHFIKEKIQNIENSINQLSIDLSRLTGKRYEELLEIRKQRVLLARELQELNEVYIPLNTMIIDPITGQDQKVSELQIEIDHFLYNQDQLCDVMLITGKAGSGKSKASERIEVELWKNHNNNQQWIPLYISLPTIKHPLFNIIDQGLEKDYYQFDKIQIQEFKQAIKKKKLKVVLILDSYDEMRSEYIQSNLFHTNKFITEFGQDIIGSNFKVIITTRKELLTIMGYQTWFCGSSLEKMKEIMLLSFNEDQSQDYIKQYCVCCIKRQIINIYETLIFLQKKQFNVKELLQVWNSLYEFLVQETNFQNTRSNQLLSNNEIKYLIKFLKCKNLFKSINDNHIQQLQQNLFNIWSINKYQNVIHNSNLQIIFNTPFMLNVVVQVLPNLTKLNQEISTLKEQFLQNFINIKKNLIESELMFMRLKNEIIQQEFNLKDIKDIGMKIIMNLDNQNFFNLYSESNTLELIDGKQLKIMNEIFNIEYDAEIVYESLKQKKFTSYQFYESFISYYHDQQINKWKKMGKIYNYETVKVDLQEFSQSLALDMTKNQVTSIEYKIKGELKIRKAQHFSQFQEEWQDQYFNDSYGEKEYKTLIRNSMLIIQNGSNYSFSHTSIQEFYVAKYIQEFIQSIQMDQDMIILQRNFNQDYIEKIESKHFLGCLNILKSEILKIDQLKLKLLSIVKLSGIYPKLERISSNFIYLLSYLNFNMNNQDINNINISDTKLYGLTFYNCNMQNTKFNNVSIDSCNFNKANLSNVEWKNIICKEKPYFQGHKDYIRSITISSDGSILISGGEDNLIILWNVKTCQQIQILEGHTNMVRQVCISHDNFILASGSNDKTIRLWSVKTGKQIEVLEGHEDSVTCVNFSQDSSLLISGSNDNTIRIWNVKSKQILTILKGHSETITSLQLFDNSKRLISSGQDKKIIIWDIVNRCQCQILQNESEVLSISLHKDGQFLSSGYKDGRIVIWDIIELRQLCILEGHRSNVNSLGFTSNGQILASGSDDQSVRLWDVKTFKQVGYLQGHSHFITSLVFSPDGMILYSGSQDKIIRQWNVTATKQDYILDGHLNYVSSLSFSPDGQMLASGSRDCYVLLWNVQEGTLICRFEGHTEMIWCVLFSPIKMILVSGGDDQTIRIWDPQFQKQLHVIKTECDSIQSLAFSNDGQVLASGSGGFSYIIKIWNLKDYSQIQEFDVHSHTINCLQFMQNGGADNKIFVLNVEKGKKELEIKIHKGQVNSLKLIKDILISGSSDHTIKYYNLEEQKEISVISGHQNTITSIAVTPDCKMLISGSDDISIGVWDMMTQKQLASLQTSDQVKCIDICPNGQIFAAGCYDGSIHLYKIIDNQKFDCYKTFSMCPLIQARDCILKNSKIQSRTDSLEPLLIQKGAIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WRC8</accession>
    <name>A0A8S1WRC8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribosomal RNA large subunit methyltransferase K/L-like methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1010075</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000101">
        <property type="protein sequence ID" value="CAD8192303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WRC8"/>
    <dbReference type="OrthoDB" id="296065at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RlmKL-like_Mtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370">
        <property type="entry name" value="RNA METHYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370:SF3">
        <property type="entry name" value="TRNA (GUANINE(10)-N2)-METHYLTRANSFERASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017259">
        <property type="entry name" value="tRNA_mtfrase_TRM11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA large subunit methyltransferase K/L-like methyltransferase" evidence="3">
        <location>
            <begin position="318"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8192303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="414" mass="48070" checksum="97B1831790903DD4" modified="2022-10-12" version="1">MIQEQEHTVTLVINQSIEMGEFGIQEAIQLFELFGLSYQELFKQYFPLNQFPKKYQITSQTLPNYPFVDIQLKDLDLIPKVLSRSILIHDILYTMSRGNTLDELPNNVLPQAMQYIQNPNLSCKCDIITSYFKITQEKRVQLIEQVFGEKGFNCQNTINLTEPDILYYLIITKDQKYIAGCTLTKKKKRREKNFARNYELPNRIYLGPVSLAHDLAFLMANQAQVQENDLVFDPFAGTGSSLVACSHFGAICFGSEIDGYLMKGHCIGYLNKKSTYLKDPNYKQVKPYIHLNFHQYNLPTPNLIQADVHLPNFNPRIESFFDAIICDPPYGIRASIQQDGNEQDLQANRTAIYKRMFEVARKVLRRGGRLVYLYPLFKGMEKKVDKEEGFELIDFREQKMVDKRSRLLVTMEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WV29</accession>
    <name>A0A8S1WV29_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1020084</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000102">
        <property type="protein sequence ID" value="CAD8192647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WV29"/>
    <dbReference type="OrthoDB" id="303810at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001447">
        <property type="entry name" value="Ras-related protein Rab-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8192647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="209" mass="24334" checksum="35A493C20AD986BE" modified="2022-10-12" version="1">MSQSQYQDIEFLVFGDSNVGKTSLLKQISGEKFEENYYPTTGIELIIKQIQIGDEILKFRLWEFPGYSNFKQFARMYYKRAQGLILLYDITDYQSFKNIEIHLEEAIQHHDSLLSIILIGNKVDKDSKRQVSIEEAMDYANIKGIHFKEISVKTKQNLNLTLEQCIIDVIEKAKIQGIELKKSPNNNQETESQSLNIVNKLKQNLQKCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WV76</accession>
    <name>A0A8S1WV76_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1010049</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000101">
        <property type="protein sequence ID" value="CAD8192255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WV76"/>
    <dbReference type="OrthoDB" id="1939479at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="3">
        <location>
            <begin position="287"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8192255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="512" mass="61877" checksum="AA49855646E3E455" modified="2022-10-12" version="1">MQHRKLFKIVEISENEYQKVQQTSQIGQAFKTYQKPLQVIETQQSEFLDYINEKLSLLLQYLQVPYKKYIISHGGHDEQNDKCQVNKIQQQNQNQIDCKTNNFNPTTTQIQLPHIDYKPKQLQIQTMYCLIQQEQKNENIKKYIYDDFQQDLQNWRTNQEKKIKKNIQDANSQCKSVVNCSCIKQDQIFEYNNQNQAVMILEMFHTKYIDEDKMIQKATIRYEEDLFNENELEQINKLEQINKLVQRNKLEQINKLEQINKIEQTNEQEQILIFQKHSQYPFVKLNQCIGRKEEQLLNETGKFWTSSMIDGYANYLFGQDEKKYFRLSKSLRECYKRLFIFTSDFITNCNLNTQPFKEKWLLLMLEKLESFKTIQYQFWLIYQNIAFIINKNNSHWYLITVNVTERYIFVYDSLKGNHQHYYMVGDLLSYLFYELQKNQLQQQNIRQDQYKFKIYVKSNFLNQSDAYSCGQYTCIALEYISNLSNIVNYKNKTDMKQNLKSILFYKKLKKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X4X0</accession>
    <name>A0A8S1X4X0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1140079</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000114">
        <property type="protein sequence ID" value="CAD8197033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X4X0"/>
    <dbReference type="OrthoDB" id="443949at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45641:SF19">
        <property type="entry name" value="NEPHROCYSTIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45641">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_6G03870)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="100"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="490"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="575"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="680"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="612"/>
            <end position="639"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8197033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="818" mass="93975" checksum="902E988302773CBD" modified="2022-10-12" version="1">MGNQCCGNQPINRDANEQAFSQAIPVRLQQKLKPESQRFHQVLISSGENDKKDIFSNERQQDFILNLKPYSHLATNDSVVVSIRVVCHDYSYKDELDWYIDESVIIDPNCFETSLMRVIDKKTGNKYTLRVINFNDYEDYEFKQALYQIYIMQLIGNKYKNLISLHDCYILENEDKEYSKGSIILLMEYCDSTLHEIISFRKFHNWQWTSDEIRHVFGELAQALGQLEQLNITHRDLRPHNIWYCSLSKTYKIGGFEEAKFIKKAQTLHNQLSLNNLGINIQQENEELLNTIRGVPDYLPPEVQKYVDASGAQTVGRYNPFLSDVYQLGLNILMMDQLEVNFESTELRNVVRKLNNQRAGSRGTSNDYHDVLKLMLVGDESSRLTPAELSLFTKSYLSEIPINEDNLVESMKYKKKYAGIESTKVYQLIAVAYYDLFEWEKWLEKMEEILVTYENDKDDFHAAEVLFDIANGFIDINNLTSAQDYFMKASILYNEIGHQLREQNKLNEALDNYERALDCIRKAHNGDDSYEGALLLENLSNGYRLQGNLARARSFLEEACRITEQEKGSDSLEFAKMSINLGKVHSLLGDYKHALKRVKKGLRITEGHASGNQNLQQSMLSYNNQNQQLQNQALLLKAQGLTMLGEIQRLKGSLGKAKKNIEEALSIREKLQGSGNLEVASTIEVLGNVYFEMEDYHQAKSSYEKALVIKKSKLGENHVEVALTLNNIGNSCKHLEEYDKSQKFFADALVILKQSQSGDDHPLVATTYGNLGILLKQIGNIQSAQQCLTKCIQILDKTLPKNHPDSLFYREQLQEIQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X5P4</accession>
    <name>A0A8S1X5P4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1050047</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000105">
        <property type="protein sequence ID" value="CAD8193796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X5P4"/>
    <dbReference type="OrthoDB" id="282419at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47725">
        <property type="entry name" value="OS03G0364000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47725:SF2">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="1">
        <location>
            <begin position="4"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8193796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="85" mass="10220" checksum="DAD849265C119C36" modified="2022-10-12" version="1">MEPLKIYLRIKRENRVFFFECSLLDPVEILKRKLLPYYKQEIQDMRIYLGDRLLDDKANLQDQAVPNDSCLILKLRKGMSGEWDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X7W6</accession>
    <name>A0A8S1X7W6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1150038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000115">
        <property type="protein sequence ID" value="CAD8197302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X7W6"/>
    <dbReference type="OrthoDB" id="305130at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="260"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="320"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="626"/>
            <end position="660"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8197302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="736" mass="85715" checksum="1EDF9B06A50F570E" modified="2022-10-12" version="1">MFAFYSDLIDKSYCPFPKLNPQQSYLNDVYMNKENIKQYKIPSKKYNVITDEQRNSLIKMITEDGISIKNASEKCSIKLSTAKAILKVYKTQGRVGKKQCRQRKARTKSKIENGRIDLMMEIRKKLPKIGFNQYLCFATQQQFSKPVKEEQYNIQIENIEYPEDDESFVESDDSELNPFQFELDQQKSKDERVEIMYRALLKNRDNILEVISDANHNLKKLRKYASVLQKEHQIFNILKKAYEDLKHKTINHDQLLISLEKGYEKQIQELSNELQQLNQQMIQQAQEFEKQACLYKQQLTQKQEQLTLKEQEIIFIKNNELQLSDKLKNSQAELVKLQKESNAIKKIKTSNFLNLNEKSDFPFSTFELVIATKIKTQLVSFLSSKDIVNLCLTKKSIYFALCQFKVVIPQIIRCSTIQYQSQMKLLTKQVDLFKRLSNDCPEQIVKTQIIKYFDANLNPNEWLDPIIIEANNFVKGSQFLGRLEHIENADSTDGLKTFVQSMATRSQQTSNLLKSDPFNLSQFTANLIINSNDREAFQKERQNQLKKLKASQSDVQEKIFNLGTAVAADVGLFGLYLATMQRIFASLYVFGSYINIECRSQSSLIYYLAEQHWQLIQKQTILESMIQDRQQRIQNNNNEIKALNEQTKGLQDLIKTLQASIKAHKIETLNEQTEKAKIKQELQETKQLLFSYGKNIKLLALEIRELRKVNKEIVMQNNKAASQISDLKSQVAQIQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X8Y3</accession>
    <name>A0A8S1X8Y3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1150117</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000115">
        <property type="protein sequence ID" value="CAD8197460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X8Y3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8197460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="180" mass="21169" checksum="4E82845B5D7222F5" modified="2022-10-12" version="1">MQHYAYKSKFLKFYTIRIIQICEQQTLKIGLKQIREHSNRQSKINKKNKNNKSKEQVYRGEIYISTRKYIQILSQFPKLTQNINLSDHLKKKIGSNSQITTNVKEKISFIIEKKNQEQEILYKKGLQNLKNHRSIRGGIQIINSITLVAIQKSIIEIKVLMIIAMTALIVTVFLRLIKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XC11</accession>
    <name>A0A8S1XC11_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Adenylate kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1190081</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000119">
        <property type="protein sequence ID" value="CAD8198600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XC11"/>
    <dbReference type="OrthoDB" id="285272at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8198600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="228" mass="25858" checksum="A4BDE8CE69A49C24" modified="2022-10-12" version="1">MNQQFFGNTRLAIQRAQQLHLFFTESVIYDQFKYTAKMPQYPKIVFLQSNPFVRNLVEKQFVQYSIVDAQKTTGEDNVARLAEQVQLQSTQTNGALLFGFPNNAVEAEKLDRLLDGVNLAVRFKLSESLAQKIAGSYLSCQSCGKVFNTSLPFVTPTHPGYQNNCQTPSKCALEQSSAPVDQVNAEVTNYYQQKGAYLEYEINEEHLLYDSQEFFEKLDNAVASHIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XHH4</accession>
    <name>A0A8S1XHH4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1240161</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000124">
        <property type="protein sequence ID" value="CAD8200477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XHH4"/>
    <dbReference type="OrthoDB" id="288185at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8200477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="76" mass="8777" checksum="B1B34EC99EBD55DE" modified="2022-10-12" version="1">MGCINSSSHQKRKKDLDINNVPIMLNNQKSQNIERIQDNWLEMKNNSILARRHQQSKLSLKVTTNSKSPLSCISRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XL57</accession>
    <name>A0A8S1XL57_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1290069</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000129">
        <property type="protein sequence ID" value="CAD8201873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XL57"/>
    <dbReference type="OrthoDB" id="299389at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF122">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN SKYWALKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="2">
        <location>
            <begin position="248"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="134"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8201873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="408" mass="48915" checksum="1F0949CD474E50C5" modified="2022-10-12" version="1">MLQIPCFQHDKSLKKFILINKMETKMLCDVCLTEINQKSNQVNPDDLIYLYNAYKTPEILLKKINISKDLLNFFMEQPNYDEFQLNHQIKILEKRVEEINHFLQGMLQELKSKVIFILELKSKIRNELEHEFQFNNFKNIIDNLEISRESLTQQILEQNEQHLHQYFQKLMSNDVENLNKTLYQILEKRRLEFENQEQFLKLEEYFHHITYKHQQLIKQIQPNFPNVLINYRSIIEQYKQKLFDIIGLRQEKTIKSNQLIYQGTRDGLQGDQFWYYIKGRSNLLMIFLSKSGYVFGAYTPCLWTPTFTGKYVQDDSLSSFLFSQTHDQIYPIKYNQRDKAIYCKYDYGPCFGSGWDIKILSDFQEGSSNHIGTTYKCDQYLIDKKKIHLFGQCKPLIIECEIFQICFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XLX3</accession>
    <name>A0A8S1XLX3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1280125</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000128">
        <property type="protein sequence ID" value="CAD8201658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XLX3"/>
    <dbReference type="OrthoDB" id="285149at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8201658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="114" mass="14128" checksum="0C199D55745C2709" modified="2022-10-12" version="1">MQQYPIRSYSKYQNQLQECTTRYTPNKFTDWHKKYEPKHKFDCQPDLQFQAQSITNQRDFQIKQRRYSQVNYPQTPLNQYHSEFSNKENKPQIQQNFDVDSYVRKLQVRLNQRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XN85</accession>
    <name>A0A8S1XN85_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1310022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000131">
        <property type="protein sequence ID" value="CAD8202407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="311225at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035751995" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="1371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8202407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1371" mass="164381" checksum="6D1060789BC65A42" modified="2022-10-12" version="1">MEILLTFLGYLIFNVNSSIKSCEDLKYQPFITCYSYISSCYILEGDNEDYSKLQHIETQIIKPSFIIPLQEQPIVIRIIDNLDEKKKIQEKLLCYLFSKIQYSIVCISFDLVIQNDQVNFIEKLRFDTNIVASEDCKEMNMNEDGSLNLFCLSKFKLKQYTLYLLRRNVTLNLEHNVQDQIQDSCQIKQMKWKENQYIIAFYQCSNWKILLINNSEVQNLLDAQMKENETFLQGFSYIHDVSFCMHNQLIFALYLIENDSYLQVNMNSLEKKFANYFSFENTKRILKIIVQQTCQIIIIVYPLQDNKQKVINYQTNKEINLNQKYQNHNIYFHSNLLFLQNQSELNILLNAHLNQTYQINNTPLHFFEFIRLFCQFDQTKNTLQFYRYFQLSSFIQPKKKYLFLIQNKDLFKSNTIYMCLRIIYENNTLDDIQTKLVQKITMQNNCQSKLKQIIWKTEGQSLLKNSQFNLYNHGVSINVSIRNNDKFVNYCLSKLQLFNFIGNIEVRQIKIPGFICFQNETQFFIYNCNENKLQISINKEQFEVLESYGDYIVVSKNNKNLLRIIQLQNDLIFQFKIEFDDLIIKAQQISQSVFLYMNNSNLPPLIYSKYQSNFNSKYLLKSLYQSEPILFYYEAENQRFIQYLNEMQNSLKLDESLIISIKVQTIANSDYLILAIHDLTRSIILYYFNDHEIHQISNYSFSDYQFVHPFKYKIKLHHLAVLMKQNKNLYIAIFECNLSSLQLQEITETDDSFFIFHQSDLLYSFNKEWRLLLLNEIIVEMMTNTIFLNNLSSFYYTYLYPPQQEDRGIQLKIQILNNCYKLYSLQNLTKFEIQQNQIIKLNISDIFYGPINSLTLVTNSSITLNGPFRLREEFQLCSLNRTNFCIKQYQIERATQILMFSLIILENKIFEILNDSNENIIFITFIKLNYYLCFFKSDNNLKIQLIQCSDEYINVISDLNNNFKIQEINRSDIKRTGNLIKLKNEFIQTFIYFEDINFILINLSEIVLDVIHIENSQNQYLILQQNQINPIDIDLIIYSINLDQKILLYSLSIYKKLQIELSIRQIIFQELKLTQCIQIGNLIIIKLFIFSKQYSYIFQLILDQQKNQITFELQKSIRNGISINSINSNFKIDYSDENILVLNQIYTNQQLFYDFSQDRNFYDYFHKSIKYFQIKRFNTTHFIFINESSIHLGTIGYEIQQQNSIEINYNFLLHAQNDVYINNYILLYQMKMHPFRYIQLNKSINLQRLKYQFNYHAQFSQLYIQEKEKFDIKNQFITSLNIHLQIINQFNEFTQNQFSCQIYNVNRIINNLFWPRIQIFIFFIQQNSLHFNQTNRLLNLSSFYIIFFLIVNFLSVKLNQLSLEFEIPSQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XNB4</accession>
    <name>A0A8S1XNB4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peptidase M14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T1320111</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the peptidase M14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000132">
        <property type="protein sequence ID" value="CAD8202916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XNB4"/>
    <dbReference type="OrthoDB" id="3626597at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF143">
        <property type="entry name" value="SLL0236 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="508"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="10">
        <location>
            <begin position="45"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="586"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8202916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="688" mass="79940" checksum="F11A6950E2B2CF3C" modified="2022-10-12" version="1">MIFILLIQITQSLSIYQFLNLTPFQIQELKIDTLQEYFMSGSISGYVEMNEYTNLIEKLANSFPKQVTLQSFGNTYQGTKIKTIKISPNKSPKYQITIIGMLRGSTDLISVNYLLYQLYYITIQLSNKNPFMMTLLQDKQLWITPLINADGLQYITNQFIQTHNIPQIFKNRNPSNSNQCSQTEQGVDLSRNFQYKFGINEIGSSSNQCSQIYRGISAFSESETLAIDNLVQQIRPNILIVMGGDQDKLVIPDSNSSTYYELLYQISKIYQLNMGEYEKLYQVEINGDLCQNYIYQINKVCFTINIGQYELNQTIPNFQNLLIDFFQHYSPSLQIQLEKVFDCKHGKMLCKELESNEKGCVIIISVQNIGYNQFNDNIIMDIILNQGGEKLYQIDSYVIAQSIKNISDPKLQDGIRIDEISTDRVYRYQIVNNRRLNQNQTIYYVFLITQYGFSYNYINTVETINLTFTLQNTLLNSQVLKTLTPSDLAAKKSNEQLQQIFPNRPESYVLLICFAAISIIFFFSWIIRKIYIWIYDPHQFIEESKSKSGGSSDEKNLDQYHSHNLMESRLNTQIKLSQVVPFQSIRQNDSPPLSKVTSQQGQHESKTTNENQLLPHLSPIEIENRVHKALEIVQSQEDLDKQEQQLQQNYYKNSQQQENLHQIEEVLVNTPNIQHNDQNDDQLVKFED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XR00</accession>
    <name>A0A8S1XR00_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1350096</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000135">
        <property type="protein sequence ID" value="CAD8203916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10035969at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332:SF175">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="24"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="32"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035713442" description="EGF-like domain-containing protein" evidence="1">
        <location>
            <begin position="17"/>
            <end position="2681"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="171"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="225"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="347"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="403"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="513"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="637"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="694"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="804"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="947"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1148"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1201"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1299"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1348"/>
            <end position="1391"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1392"/>
            <end position="1421"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1491"/>
            <end position="1529"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1537"/>
            <end position="1578"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1579"/>
            <end position="1626"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1634"/>
            <end position="1673"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1681"/>
            <end position="1720"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1721"/>
            <end position="1768"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1818"/>
            <end position="1867"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1875"/>
            <end position="1912"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1920"/>
            <end position="1955"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1963"/>
            <end position="2000"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8203916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2681" mass="304643" checksum="582C51E584934B2D" modified="2022-10-12" version="1" fragment="single">MILWFIVLQIIIDVLGFQSQYWYRTNLKIEQKFCPDLHVTVPDMNPHLCVSYAEICKDVPEADVLVAQNGTQYICHPRFQPFAPGTPTIDHYGALICGYDEGVPLVIELLQDNHLKFYCKFIEGENYKNCLATELINGIPRCTYCKAPFYGEYCKQQINGFDWRYFNPPKKCKFENCIECEDPLTCTKCFPGYSSLNTVVTCNIYVGLFPPCEKGQVEDQNICYYCPAGCLSCRKISTIQHVTCDTCDANYFRLNKNKNDPDWYFCIRLENCDFSRQQILTDQTTLDEQGFYIYTITCTQCDLGYYYKKDIQSCKMAIQLHHECYIYDPIQQLCVVCFAPTQFRDSACRYQQCDQAICLTCSTLYPNFCTTCDSSLEKGGVIKEKGICSCKQNFGLKNDVCEPCSDGYCQECEQDDFYSCTSCALGTNRILVNKQCICQPGSFEPEDGGLICIVCDWSCQTCFGPTNYECTQCPDEIISNRIQVGNSCPCKFGYSDYAIQEQRCGKCHPRCQTCFQAEDDTINQYCLTCIPGQNRMVNDNFNCDCIENYGDINGTKDICFICHYTCGNCDDFGSNNYNIECSKCHYSCLRCANSTEFNACIECPSTRIPSDSSAIQFECPCLSSHYYDDGLSPTCIECDVSCQTCFGTLSSNCLTCDPIYRQLDLSSQKGGVIKEKGICSCKQNFGLKNDVCEPCSDGYCQECEQDDFYSCTSCALGTNRILVNKQCICQPGSFEPEDGGLICIVCDWSCQTCFGPTNYECTQCPDEIISNRIQVGNSCPCKFGYSDYAIQEQRCGKCHPRCQTCFQAEDDTINQYCLTCIPGQNRMVNDNFNCDCIENYGDINGTKDICFICHYTCGNCDDFGSNNCTYCSDSSFRYLTTSRECLYNIECSKCHYSCLRCANSTEFNACIECPSTRIPSDSSAIQFECPCLSSHYYDDGLSPTCIECDVSCQTCFGPLSSNCLTCDPIYRQLDLSSCICPIGYYDVGQLECEKCHYSCFQCFDNTAEGCIACSIDLHLRVLKGNTCKCIDGYYEESGTAKCKKCSYKCETCVDQPEKCLSCPFNSLRILDIDKGCYCAGEYYEKENEISCQRCHFKCKTCDAFSESSCLSCDLIAFRELKLKQCKCQPHYFEMEVQECAICSAYCYECINNFDNCTSCQDDRYLVGSTCKCSSKFQGAQISTFEFNGQVQCQKCHYSCGTCGGIEESDCLSCIDTDQRYQLGNTCICKEGYFDAGLPVCQKCSYKCKGCMKQSEICTSCQDNSLRQLLSDFNICQCIQRYYDDGQNEVCQKCHYSCLRCNDIDTKCELCSIESNRIYNDQLFSCDCNVGYYDTGVEICQKCHYSCFSCNFGDPNSCNSCVDSMTSYRVFYNDSCQCISGYFDNGQSVQCQKCDIQCLSCINQSYQCQSCPQTRKIESNCKCQQGYYDVGLQLCLKCNSNCFTCESTSNNCTSCDSDQFRELNSITQTCDCQIGYLEINGICEQCHSSCKTCSQSLNLCTSCVQFRYLKNNNCICNDGMYESIADKQCKLCNKSCLTCAYSDIYCLTCSIDNFRIFKSGNQCECKLGYYENPITQQCEECAKSCLTCSITYDNCLSCDTNLNLSMINNKCLCSQSYYFDSLTKSCQQCHINCLECQNNSECTSCRDTTRYLDEDQKKCLCNDGFYETNQQNCQQCHLSCETCANINTNCLTCLNITYRILKNNKCLCIDGYYDTGIELCQKCNNICKTCQNTASTCLSCYEIEQNRYQLGDKCLCKSGYFELNTDICSKCSNECLTCQGQADYCTSCDINSKRVDQSIIHKCPCIIGFYQDQNQICQKCHIKCQTCINQSDQCLSCKFELNSNRKSLSDQCNCKEGYFDDGIQLQCQKCNLKCKTCQNDSINCVICSNSIRINPPTCNCMDGYFEDEQFTCQICSSQCNTCAIQPSNCLTCKSGRIGNDCKCIDGFFEFGSIFCEQCAFKCATCSIQAMNCDSCKGNRIQEPQCICQPGYFDDQINEDCQLCDSACLECNIKGCLSCNANRILNEEMDCVPPPNSIWYNDTPWCSTCEVAVVKAYLSDDISKIIIHFDFPLNSKGFNQQFELNKCLQLFELKSLQSFGQNSQCFINPDDNHELLISLGENSKINVGDEILFISNSLSHINCETTLNKFIFTTLQMPIKPFAPQVEYHVPVHQLNPFTKNSVYLKSIKNYGNRKLDNIIWNFQVETNQDTQPLGDFIDQMNFLQEYNLLIPKFTLPSDAILKFKIQYQNFIHIQSYSEFIIYTHSGDLPYIDIIVKPSYFVYETITIGLSAGTLFKSNQVDNSKYMVQLSEIDRHPKKSFPSRLNTSLETNPFEKIQANITKYTLSPNSTYTFQVIATNLNTNKTQYQNLSINIPFAGLICQLNNRGVQNIRKDLNLQVKCKDLDTTYDWNNDPDLYIYVTCKDLTLNNSCLNQQKQMINVNVTDSVQFIKKNTISVFTVQEWSVIVTKFQQTQKFNQIIVYLEDDFPVLDLEFNQGYLMRKVNNYEQLNFTFLIPFNQKPFLLDLSIAIIYNYEIIEILQPKYISHQFKLFNSIKELNFGDSISLKLTAQYTNNIMPSLNTLKLNINQPPFCSKLIITRSNDLALTNMMVATSCEQSNDSPYKYQLRVFLRESDLTDFLKGSSDNSLILYSFQYQTQFSIQIPTSVDSSKIGLLLQVLDSX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XTS1</accession>
    <name>A0A8S1XTS1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1350150</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000135">
        <property type="protein sequence ID" value="CAD8204018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XTS1"/>
    <dbReference type="OrthoDB" id="2160519at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="3">
        <location>
            <begin position="388"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="40"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8204018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="587" mass="69761" checksum="E0D14956889FA4BA" modified="2022-10-12" version="1">MQQQDLQQSERNDSQIHKKSPEIHEFISKCQLQDSYISIFNESVDLQMELQKEVNNLQQQGQLEQLTQLESDNNNQNAQFSQMQQQYKQFEINSEKMKKLIKAEENYEQLKIIKQQKEDLQQQIEDEKLVFQQIQQKITCLEENMTASIQMLNQQVSKQEQQQQDFLQQINQEIVTNNKLKDNLQELSNSSEQKLQQLATKYDDLNQYIDEAQQEIYGMKNSTLLQDKLQQLENSIDTKQKAIHEKIEELSSNIEKKNIEQCQKLQQEKQELLKKINEEKIETNIQIEQQNNQFKQIIKENKDEFTKQEKSISSVAQSIKEENWANAQLKKEIKEFGDQNQKGISQLAIKIDNQSKELIKINSEMEFWLRDKIIQYHLKTRQFRGLKIIYEITQKTKKTIKGSRDIYLGTRDGLKAEYFWQKAYNLENLFMVFKSKSGYIFGAYSPFKWDYNGSSNKQDNTLSSFIFSQNHQQEYHLKEDKKNQAIHCDKDYGPSYGNYDDIKIAGDFIDGYSRLGTAYQFSSQKGNSNNPFLFGQEKPEIQNVKYIKYNFVEPIILDQQQFNSISYYVYNYELRIFNQYCSKKRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XUA6</accession>
    <name>A0A8S1XUA6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1380088</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000138">
        <property type="protein sequence ID" value="CAD8204780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10605345at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052986">
        <property type="entry name" value="VLIG_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14819:SF25">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_52, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14819">
        <property type="entry name" value="GTP-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="307"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="704"/>
            <end position="731"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8204780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="990" mass="119664" checksum="583C4F5B71CA87E9" modified="2022-10-12" version="1">MKFFSKEYPIPFLYLNPTMDNLRFKTEILLLNSNLFYFIQQGYTILNFSLSQNQKKIGKTELINQIFYEREKFQTSDTCQMNQNTIDIMFDFEFNNSRNFIVADVHGTIIFEILIKILPFFQFWIVQMDSLKEYQENLDQISIILQKIEYQIKQKLEICFIVRNTEERELMNNQPINYEIENKNIKIFYLHNLNANQIDKQFKISQIQDVSKFLFSLILDHQKRLQQSNQYQENLKNQFLLVLQQFNPQYNVCIQQIINHQNLINQIQDKLNGIVNEQDGFYSQNAFPIRRIQWNMKKLRNQNYELCMKTIENKMEIEKNKKQIEDQEIQIQKQNPTQLLKIFCEIFKQKQQXDILIYANQILQSYKQIVDGIGCLNLKSLFQIFFNNANDELRVILMKFFSKEYPIPFLYLNPTMDNLRFKTEILLLNSNLFYFIQQGYTILNFSLSQNQKKIGKTELINQIFYEREKFQTSDTCQMNQNTIDIMFDFEFNNSRNFIVADVHGTIIFEILIKILPFFQFWIVQMDSLKEYQENLDQISIILQKIEYQIKQKLEICFIVRNTEERELMNNQPINYEIENKNIKIFYLHNLNANQIDKQFKISQIQDVSKFLFSLILDHQKRLQQSNQYQENLKNQFLLVLQQFNPQYNVCIQQIINHQNLINQIQDKLNGIVNEQDGFYSQNAFPIRRIQWNMKKLRNQNYELCMKTIENKMEIEKNKKQIEDQEIQIQKQNPTQLLKIFQQYYIVYLQIVDKIRQFNEKNLINLQQKDYELNEQFNKLKQEKHKIKQKYLNNLQNLCEDQLNKTIKDKMSKLKQQMDNNALNISKKNVGIELFWRELISLRKSNNYDLEFNPVETLSELIRKGESLEFLDGDTLSINTQFLFDLENKITYQVLKVQVNQLFQTKSLDAIFGLVLEDVQKEFIYKYYKFKISKYFKIVLIKYLFLIQKVYKIPIKLMQNLIKNCIICAFNQIYYYDKCKRRDQLIIQKFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XXJ5</accession>
    <name>A0A8S1XXJ5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1400012</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000140">
        <property type="protein sequence ID" value="CAD8205178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XXJ5"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000085">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="227"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8205178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="526" mass="59690" checksum="D4404D969536905D" modified="2022-10-12" version="1">MNPIRVYPLKFNNQDSLAISPGLKKKQQPSERTHTLQINPQQTPQQSRSNQKRHPTNGLTSQEEKIKLVTQKKLQLLQPSSYSPKKRVISSNNDKPELSKFSYQSTEPQSPTKLPNLKRSESSTTTQEILAKIDLSLAQKRSKRTDTSNADNTPQNKTSFLKMYQDTSPKIRQIQSRVSSQAIEQDIKIKPRMPSQVIMLHGRSTSQAALVNSNPTENEEQQDYVQLFLSKSQAGQNGNKQTKTNQDSVIISNNFCGMKNRYLFSVCDGHGVYGHYVSQLIKRVLPTIIEAQLKTFIGKQEIDIGEEYYTEVVKCFNSSFQKMTKDLQNCGIDITFSGSTCSTVFVSGNNLWCSNIGDSRSIFIEQHRESNKWKIVELSNDHKPDLPTEKKRILASKGRVQPFVTENGQNIGPARVWLLHEQIPGLAMSRSFGDYVASTVGVISEPEVIYHKLNQKCGFLVVASDGVWEFLSNEEIQQIICSYWSPQMNAKKIDEMVDNIVRESTKRWQEEDEVIDDISIIIAYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XXQ3</accession>
    <name>A0A8S1XXQ3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1430071</name>
        <name evidence="5" type="ORF">PPENT_87.1.T1430073</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XXQ3"/>
    <dbReference type="OrthoDB" id="282736at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048280">
        <property type="entry name" value="COX6B-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02297">
        <property type="entry name" value="COX6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8206182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8206186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="99" mass="11833" checksum="8B5C1F66F8E46384" modified="2022-10-12" version="1">MSYNGNALLTPSRYGGESWNSQTCFTAQEDYFECLDRENSTNVNRKLCGREHQIWKESCPDSDRRAQMIFRRLDRQNQALYTSEQLRLYNNHQNNRSFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XY39</accession>
    <name>A0A8S1XY39_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1440054</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000144">
        <property type="protein sequence ID" value="CAD8206479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XY39"/>
    <dbReference type="OrthoDB" id="26681at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06071">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000409">
        <property type="entry name" value="BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050865">
        <property type="entry name" value="BEACH_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057496">
        <property type="entry name" value="FAN-like_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023362">
        <property type="entry name" value="PH-BEACH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743">
        <property type="entry name" value="BEIGE/BEACH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743:SF123">
        <property type="entry name" value="PROTEIN FAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02138">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25400">
        <property type="entry name" value="PH_FAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01026">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50197">
        <property type="entry name" value="BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51783">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BEACH-type PH" evidence="3">
        <location>
            <begin position="226"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="BEACH" evidence="2">
        <location>
            <begin position="328"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="620"/>
            <end position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8206479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="733" mass="85832" checksum="15EDEA9D78C8EBC0" modified="2022-10-12" version="1">MFPSINSIKKPKKNFNLLHLETQEQYFLSLKVALIPSTKSDEIRGKLHLCSRSLVFEPKSSQLPLMKMKYSNGLFMKVLKNFEPKSLGLLLSIRWGHQLMQEDIDQLDLASILNQQKGEQKSNELLEKLYNKQRKNMIQLACNCFFIKVDKLFIINRNPISPYITETCDDNILFTISKSNSKQNLIRFLKLYQGINQAEDEFTFINQTIQQTLNDSLLEHKQNVGSEGHKIEFVHKVKLIKPEGVIFGLFSIQRDEILQFIPLINSPQGKIMQYMFTDIKYFLKYRYMFKNNGIEFWMYNKKRSYLFVFDDQTTQESVFKYFQKKASKHCLSAFTKEKITEEWINGNMSNFDYLIALNTLANRSFNDLSAYPVFPWVIAEYNEKDFNVNSPSFFRDLSKPMGALNKHRLQKYKAFYKDQLKDKKQDPKPYLYPTHYSSPGTVVYYMIRKIPEFVIKLQNGVFGPTDRIFRGIDSTWFTTLNLHADSKELIPEFYGLDSDFLVNCDKLELGITQDGEVIDDAVIPAWADNAPDFLAKMRIALESPYVQTNLPLWIDLIFGNKASGNEAVLNDNLFYPYTYVENVNWDKCKTDIERQALEIQVTEFGQVPVQLFQTAHPQRKLKIQGNLKQLEDEKKELKQQNQNLQNKIQKLTQQIQQSQDKELVLQLQTQLAALQWENEKLKFNLETFKQEMITNKEVIQTFNSQFDQEEFDKNGFDFLNDDSLLDSSKKPIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XZN6</accession>
    <name>A0A8S1XZN6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Palmitoyltransferase</fullName>
            <ecNumber evidence="10">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T1440033</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="10"/>
    <dbReference type="EMBL" id="CAJJDO010000144">
        <property type="protein sequence ID" value="CAD8206437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XZN6"/>
    <dbReference type="OrthoDB" id="9909019at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF43">
        <property type="entry name" value="PALMITOYLTRANSFERASE APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="139"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="177"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="11">
        <location>
            <begin position="95"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8206437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="263" mass="30262" checksum="6403E71D3F450965" modified="2022-10-12" version="1">MTGPSSDNTTNIITWLLILLVGAPFNFYISPQIWIVLHPSLPIISYVMYFSCVLFLFLTQFTDPGIIPRKEIIEKMKDENLLHFIPTEDDNADYQVKICVTCMIKKPPRSNHCAECDNCVDVFDHHCPFVNNCIGKRNYAYFISFISTLTMAAISFGIEFLSFVILIATTDEKVQQILIIVLMIPFGICIILIFGLLIFHIFLIITGKTTKEQLKNLEMSSTNRKCQRTKSLFNCRTIIEKQQYDQIRYDQDLIKINPIVVPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XZS2</accession>
    <name>A0A8S1XZS2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1440053</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000144">
        <property type="protein sequence ID" value="CAD8206477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XZS2"/>
    <dbReference type="OrthoDB" id="10298016at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="102"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8206477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="137" mass="15976" checksum="92EA68141418624B" modified="2022-10-12" version="1">MKIRQPLYHPVVQQFQCPTQYVSFASPTQRFSQGQRLASPTSQQIDYQRLRNAQSIQQSAYANKISQDIPQHMITKKPHIKMPQQGVQLSQSQSFSYNPQMMVGLIKENQEIKAKIIEKEKQLILLQQEIDLYEKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y024</accession>
    <name>A0A8S1Y024_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1470023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000147">
        <property type="protein sequence ID" value="CAD8207331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y024"/>
    <dbReference type="OrthoDB" id="296220at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37028:SF4">
        <property type="entry name" value="ALMS MOTIF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37028">
        <property type="entry name" value="UNNAMED PRODUCT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="359"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8207331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="385" mass="45315" checksum="4FAB969B593472CF" modified="2022-10-12" version="1">MVQFVNEFVEFCRTIGILEKDQIFESTQDDLGQMLFMHLVTLNRQSLIDLSNRVSQMWLMQQTNSKPIHPNSVKQFTTRSKHSQSTPQESKSLTNSNNQSTVYLYDQYIKKEEKQIIHQQKVIEDQLKECTFQPQISKKSQQLDTTAPVYDRLSQFGYDQRMKHQISMEIKTKSDLKQCTFRPQVNHSFSKILEGDPFSRLYQNALSQRQTKPVSQEKTYTFKPQLISQPIVQQEYLSIPVEERLYNHFFDQQQQLVQQQENENQAKLDECTFTPNINQYGSVQQGSEQKMRVFDRLYNKSSAVKCASEIKEQNPNHFSISKQSDKIIKKANGDNSKYVSQFQIDQTPFDRLHSEHKRIEKKKKVKENQIFSSIPFKPQTNNNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y3D9</accession>
    <name>A0A8S1Y3D9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1430107</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y3D9"/>
    <dbReference type="OrthoDB" id="299433at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="112"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8206254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="198" mass="23070" checksum="3FFCBE2FDEED91A0" modified="2022-10-12" version="1">MKTIMSTISNISHNYRASCKSEQSITPLRVPFNGGSQQNCLSKKSYTSNNILESVLIEYKQLCAWVIREINLKIDHNQMINNINKQLFQFERFLAKNLDGYGQEIQALKLVHQEHQITIQKLSSTNQDLKSKLKQISNNINEDFLSNTPLQKRHNYSIELITKQPLRNISYSLQEPSCDKENREIFPKKKSLKPKFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y5L6</accession>
    <name>A0A8S1Y5L6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1540060</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000154">
        <property type="protein sequence ID" value="CAD8209315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y5L6"/>
    <dbReference type="OrthoDB" id="307538at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF8">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="235"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="337"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="405"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="571"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8209315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="708" mass="83123" checksum="7FDA96E1692D57F8" modified="2022-10-12" version="1">MDNNNFNQNILTLKCVNHQNFQAKYFCLSPQCQQRRLICQNCLNAIHQQHFDQVKQISDLFILLDEKSQLAEQFLRQYFNQFKELQEIFSRTIKALQYTFGKLSDQLLEMDQNQTISFLDQLINFDAINVFLRDQVSNKLLKISEMLSQILQKLPLQNISYRMPRQLEQYLQQGQQLRQQNLNKEAVILFDKILQQNPYNIQIMLEKGECLIEQNLLHEAQFQFQEILYLDPRHKQALYKNGNCLLKCNKYDEAIIQFKKCLNIEQNNFEALLGLAETLRKMNQLIQANQQYDKAIKINQQSQQAFYGKAECLKLLNDFINSEEMYKKSLTINSKHFDSLFGLADCLKRSKQFQEAIIYYDQSLQFNPQNPELLEGKATCLRTIGFNTEAVPLYQQALKYDPFSAEILKGLGASLRNLHQYNEAITFFERALEINPNSSVSYRGKAECLRLLRKWDEALVNYTQALRYDTRQFEAAYFKTQLEYIIQQLRNHEQNLSINPQNFYSIFGKAECLRLLNDYEEAEKFYALAINLNPQYYNSFIGYGWCLAIKNKIKEALDQYNKALKINEYAVDAIWGKGECLRLMKDFKQALNYYKKVLILYPNHYISLSGIADCLRMTQQFDQALDQYQKILQFYSRPCLLYGKAICLMNQRKYQEALQALSLAKGLAPSNQYIEKAIQFCKTLQQIPQICQIILSLNGVGINQNQNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y6T6</accession>
    <name>A0A8S1Y6T6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1530011</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000153">
        <property type="protein sequence ID" value="CAD8209003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y6T6"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histidine kinase" evidence="3">
        <location>
            <begin position="279"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="618"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8209003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="748" mass="87142" checksum="2B12E73F076D6551" modified="2022-10-12" version="1">MMQSLIRSQSRMTIIQSPKKFFTIQLKELPDELKLKRYSNLTSLSKSQKNILNQGNSNNQMLQLYQNLINLFPYGILIINQNQIINYINNKCEKILECQGSEQVLEKVKVCVNNAKMQDNVSETSNIQQKKQHHYHLLQQIARKLQLTNNPVDILDIILSPQKYFSILGLKEISLQREYQASFNQQIFIYEWLLQSELLSNNSQKKLKLIIIPTSMTNQQQEYISASSQIKSSSKSHISNANSDKDNPVLLIIIKNVTNKYKCKQMRDESIINHSLIKSFSHELRTPLNSCNQMLNLMKYEQTTQEFQDYINIAQCSITLLIHQINDILDYAALQSYQFHYHLVNFTIQQIIDEIEHLYKLQTQQKNITFKIKVSNYLIGSNFRNDKQRIIQLLVNFLNNSIKYTHEGGRISLNIIQECQQYINFKVKDNGIGIDEHKLNQIQNSLHDTIEFGAVLKSHSGSKQAGLGLIIAAKLIEGLTESKDNKLIITSKKNKGTIVQFQIEDLQLQSNFPKQQNEQMIHQENQLHIQTQHQKNIIIVHNNNLKFENDKNFQEVKSESFMTLRLNDSEKQHQILLPISPDYFSKKNLDYYNNLQKQFQSDFSQKKEEDLLCQKCVHILIVDDIPFNQIALKMILNKYKITVDQAFDGFQAVEKVKQKMFKHCSIYKLIFMDIEMPGMDGFQTSKYILEITSNQTFIVICSAYDTQENILQGKNIGINTFIQKPVKQDQLNVVLRTIFKIETNSNFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y7E6</accession>
    <name>A0A8S1Y7E6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1550015</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000155">
        <property type="protein sequence ID" value="CAD8209510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y7E6"/>
    <dbReference type="OrthoDB" id="309707at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8209510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="419" mass="50344" checksum="98B38CBCD3BAC39A" modified="2022-10-12" version="1">MQYFSYTSEDIYTYQNLEELKKIIEIDEIGLTRIHEQEQYVSYEINREKCIRLSDLVYFISFNLVRIHLGQLLVLVKSLAQKVQQLQLVQIDHEYLDCNRIWLKFNNQNEAFNCKYSILNYTIHFTGYQCPNYEDLNEKEIERVSSSKKILIIIKYIIDNCFDKIILTTQPKDDVKKTIIEPIINEINNKQLDQLIYKIDDTLKKYQYDKQKQIISVDEDIDTINNKRHEGKVKYNLDIKKIMQTTSQMEKTPSNAYKLEYCLYSSLPQIVKQLDNSEGYQRIQYEGNKIKTAIEQIYFDLQNKIQNYIEKQVKQATKEQIQFNSQFFKFQYDEQDITKIITKVICKHAIIQYFYNTYTNIFKYTFKLNIDNQDTYQFNANKLYQAMEKAIDKIIPLETQQYFILENTNQIFELIKDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8A7</accession>
    <name>A0A8S1Y8A7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Amine oxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1570054</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the carotenoid/retinoid oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000157">
        <property type="protein sequence ID" value="CAD8209989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y8A7"/>
    <dbReference type="OrthoDB" id="7777654at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10668">
        <property type="entry name" value="PHYTOENE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10668:SF103">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="2">
        <location>
            <begin position="57"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8209989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="356" mass="39779" checksum="AC06BD2CA311EEC3" modified="2022-10-12" version="1">MSQSSSQFLNKWFESDILKGTLITDGIIGEMISIDTPASAYILLHHVMGEIFPNSKGEWAFVEGGMGSISKILTKLNEQKGVEIKTNIGVEQILTSNNQVTGVKCSDNNIYYSDTIISSVTPRITFEKLLSNDQQTKNPKYFNDVKHMEYNGACTKFNFAVSEMPKFKCFEGTKYENPESVLIGTTHLGCERLDTLRYAYQDCINGKSFSDNLFVDLVVPTVHDKTIAPQGQHIVQCLVQYTPYKANWNEQIKQQLKEQVIDYISQYAPNFKNSILYTDMVTPQEIEQLLNMTEGNIFHGALTLDKLYGNRPSNGYNSYGTPINGLFLCGSGTHPGGGVMGAPGRNCALYINNYVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y923</accession>
    <name>A0A8S1Y923_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1570110</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000157">
        <property type="protein sequence ID" value="CAD8210101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y923"/>
    <dbReference type="OrthoDB" id="10006270at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="97"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="131"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="267"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="335"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="403"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="437"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="471"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="539"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8210101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="600" mass="69567" checksum="BD1687AF627812CD" modified="2022-10-12" version="1">MKTCQNSSESFTIQSNYHTYIKKMIQKDEQEQRQQCKITKRCQIIDEIKQEQTDVIDKDLLKFKQLFDERNYDEAFLEIEEKIKQNELLKENKKNNDELLMHKSNVLIQLDKYDESLECINQAITLNPKNEKLLYHKGITLVKLDQYQQAFQCFDEAIKINPDNQASLIYLGITLEGIDNYNGAYKCYNEAQKYDQNFQLLNILKASTLIGLRKYDEAIQLCDKAFSNTDLDKFAYTTKGRALLMQLRYEEAIEICDKVLQIDETLAEAYDIKGRILASLLRPEEAIQQCQKAIQLDSKNYVPYSTIAGALIQLRREEEAIEYCDMSLKINPRGDIALGNKGIALFDLNRFEEAVECFDQALSIRQNHPIINLLMGTALFNLGMEEESIQYFDMAIENNPKCFDAYLRKGMSLFNMYRYEEAIETFDQLISLEPDNYQAFYQKGRSLINSGRFVEAIVSLNKALKLNSNVFSIWNSKGRLLLNMNRQEEAILCFKTSFQLNPNDNVAIEQIRETLESLNRIEEAIAFISQAVINNPQSHVAKFQMAKTLLRIGSFEEALIFSQQALSMQPQNQQYLQLYCKVVFMQQLSKGCLEQNEQGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y940</accession>
    <name>A0A8S1Y940_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1500104</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000150">
        <property type="protein sequence ID" value="CAD8208362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y940"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000051">
        <property type="entry name" value="Protein phosphatase 2C-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="86"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8208362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="365" mass="42344" checksum="19A668A1D98DC2B0" modified="2022-10-12" version="1">MKQLNINKQKLEPLNLPVYHQQQHQYSLQIRKPTYSLHEQTTSRQSHQRKKQKQVNVTQNSPPKYQDQQILVDKFGLNERFQYTLRISVRTKQGMIIGNPTKQNQDSYILLSNISNKTYMHYFLVFDGHGVNGHHVSNFLKQQFQLYTTQFSQLLENNPQFAIQTIFTHVSQALNQSGIDLKYSGSTVIGLFMLHNKIYCSNLGDSRAIMLSRHNRWILKYLSRDHKPQCADEAQRIINYGGRIDSYRDSQGLSYGPLRVWSNANVPGLAMTRSMGDQVAKKVGVIDKPEIFNFTMDIMDRALLIGSDGLFEFLTQQDIFDAITPYLNNIEMACNHLLEMAHISWLQRGSKMIDDITFILIFLQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YA01</accession>
    <name>A0A8S1YA01_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1590045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000159">
        <property type="protein sequence ID" value="CAD8210401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YA01"/>
    <dbReference type="OrthoDB" id="302934at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="308"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="241"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="308"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8210401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="878" mass="103870" checksum="BDE35586EB82D51F" modified="2022-10-12" version="1">MEEILEIVPNTIKFDYQSREQTISIQNLTLHYIQVRIVSPNPTLFAIIPNIFSIEPCTTTDVIVKLNLDFKMNFYSLIMNRKLTIFYYDVDDVEELGKNDMDWHKLPINFLGRSEINVDYNTNCDKYQILKSEKQKKFMESVQIFQALFIKQKNISLNDEYKKILINKYLELHNKYQTYKIMEDSIIFIGNNLDLENEIYCIENQSQIYYVSSYKPTYLYHAFPQYPIEPLSPQSQRKKSSQQPQQIQEPIKLTKSESEPPNQHSLIDLSPVKQFFSVFTSSKQKHDETTEQQIQVEQPIIQPQLIESQKMQQQQYETREQQQVTRQTDSINTKEDKQKIEQQQQQSSQQQQQQQQQQQPQQQQQQQQQQQQQQQQQQPQQQQQQQQQQQQQQQQQQQQQQQLQQAQKQQPQQAKIQQKFYNQEASVPETNRINKRLINEQNNGQKNDQKALSIDGRRSLQIIDQKNQQRLNETADLSWDQKAKLQQQQMTSPPVEFKGIDNIQNQQACYKINELAQLKTYQQITDVLLFRDGKYLITVGEKNDIKVWQLSIRGLVGTIKELENKKERKILLIKEWRYVDPSADENYEFELKDPKNEGKSEELYTPYETAYRFVTGSEDQIFRIFNLTTMTLEQSFNSRLQIPTAITFMPDRGLFCIGGQPYKTIFGQTRKYRSIRFFLMKNLLEKPKYKIFCKSEDEYLNQVIYSENPISNQKEQYHARINNILAIQIIRRNSQGKQILQSDRILFVFLDRMNQCTVLLTLECESLKIRGINDLKYLYKEGVFAVIEANDVVSMKNTKVEQNTQRVVLINLKQLQTSQGRESGIECNLIQEQRSKLSVQILANPILLVIPQPKQIIFQNFNGEDQIIHPLEIKDSKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YA19</accession>
    <name>A0A8S1YA19_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1590087</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000159">
        <property type="protein sequence ID" value="CAD8210480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YA19"/>
    <dbReference type="OrthoDB" id="288729at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000810">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF68">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ATG1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="19"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8210480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="293" mass="34548" checksum="3A1C71BCF1E4FC7F" modified="2022-10-12" version="1">MQQNPSQFDLQKFQQNYQFNNKEIIGKGFDSIVYKVIKKQDGSLYALKLTSFKDLQHKFNFQQEIRLLYHLDHDHIVKFYGNSLDGSCILIEYMPYGHLAKLIDIGLDLRIIKSIVNQVMNAIQYLHSRNVVHGDIKPENALISKDFVIKICDFGFAIIANQPNTKLRGGSEGYTAPELLINENYDPKKCDMFSLGVLFFVLYMGYRPFLSTHPQKQDKFWNFIKNKEWDKFWKIFDPKKQDVGFKNYIQKLLCADPNERYTIEEALQDNWFKSNQYTVKELAQQLRMLLIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YF41</accession>
    <name>A0A8S1YF41_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1680052</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000168">
        <property type="protein sequence ID" value="CAD8212450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="296386at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF73">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="776"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="892"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="937"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1167"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1217"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1247"/>
            <end position="1266"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="1769"/>
            <end position="2047"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1057"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1350"/>
            <end position="1406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1057"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1085"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1358"/>
            <end position="1369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8212450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="3568" mass="421101" checksum="83F61D452A745271" modified="2022-10-12" version="1">MSSPLPLNKSNDLDQQGIKQISGQINDQFIDTIFNIQRQSVDQEPKRELKQRVRYTEKLSKIYYLYLKLIMGRQLNDEEDEEFLEYLDHRSTEKGRILKMKNSFEYVDEFDYHMGDVVLSFPNPDSPEVEYVSQMSPMEAITLCMNSFNLKNSGTDFEVIDNLVFLKAIEASIFMLSQVFDDDEAPIEEVVQDSDDDEEKQKSKKKKRTNNLMRAGGENDKISMAAMKIDNKLRAIKQSVKGNKLQAIKEIFNLDYDKLNKSKIKYKMAKGNYFQYDSVKKEWNRTKNDTEDFTTLIRHIVMIQLSKTGFNSRQFLSKDGSIIFVVLYQCDQNLGIVAQTHSITKQLYMQFSDLLSLEPVDKNLRPLRLNNRLWRDQEEYASENLSENFLYLKPKIQELLKEINFKKIARELNLSQLNQQLFSKEGSEDIIEFEGPTDAMWSAYYSYLMYLKQPMRQLREAFRISNEQALTINKTPYHLYKQRFPNDQMLENLKMSEIKKLEEFVSEDQIEQEIELHGRPNEIYRVLKGIFSIVLEQREIQNKYSLIKSYFHLPSYKKITSRQIKASKREMLAMNYLELLQEGLEVTNYLNRQLFTIWDIIGIQPFEPFIPYNITPKNVAFSSQIKMNSIWKKYRVTETGRISLFSTMERMKLNNLALGEKINFSILFSRHLAISAFCLSDDFELFGKTNKALFDPLFEEEYDHEILLKDAQYSYFYRELKHHEAQDKRGPNKYILNDYSYVTPKLENLAFIWRFEYKQPWFIPTQQIREYYGEKIALYFTFLGYYTMMLLPIGFIGLIISIIQLLVWNQNDYNYIMPQIIFGMIQIQWANLMYDLWQQREKVFAAQYGQGEGGSGEQAQQRSDFMGNYERSIENDNLNTLNYSDLKKFMRILLTLIIQISILGIYVGIVIGIFFISEELQNSERIKSLIGNEVAQLSVTIPALINLVVILILDPFFDKTAGSLTDYENHKTITEYETNFVFKKYFLSFFAICGPVVEIMFGHEKVGLTCINNDCLKHAQYHFSSIFLLQFTLNIWEIMKPKIQVLIRQLSEKKDEKAEEQKTKKLASRIDEIRDDDEDGRNEDLNSQKSQSLSPDRASSKKANDQNVSEQQDTQKKETLTRDTNPFEKINNFIENEFLYKESYVISPERYGTVDEYMEITLQYSMLVLFGTTFPLCFFIAFIWNVVELQTDKLKLLNDMQRPLPLGESTIGVWNDVLEGLAYLSLVSNSGLISYSRDRIVDYNPTISLLAFFIIILLSNFFLRYIEGSIFGEIPYEIQNLMQRHQYLIKSTIEKFMGSGEKQSSENQGDEDIKRYPTQKIYGCLLQEQKTNQYEEISSDSDVEDYIVNKQKKKEKQQQQSQNTNSPKSMKSSPRSALKSETSKKKTKSKLSVSTTMEEEDAKSKKKKESIDSLKKYLATRPSNWAMKFKLKDSNDPEIQQKQKAFFFKTLKFLKMLNELNPHQKLWTDDRFTFRKCFLRRHLVLIRNLDWRRTLIFQENQNKLRHDLKESQHEKYRRQLTILNQNLDIHSNFIEKMEILKKIEEYVEKHLWIDGRKTIVFKVQGVWFSQYRKSTRKQPAIAIAKNYVKLLTNYKSVFKQQGREIDTKPIDERFYSYINSMTQTQIQKYSDEDYLKFVDSIQLFPKTNLQFPLTSKGVSQVEQELDAYIGLDLRKQFNLIHFTYIPFRIQAPQVSILRNQIWNLFIDQQIAKEQPMLYFIVPLDTKKSLHTATLEYNVFAAKQQLYRWLVSQVQKPKLSDVMVFFQTEILYHRTLHKDIINRVARFYEKITDIRYMELQLKVPISPLKYLKITLEDPIVNIRDPVIKGYLSRAYGAPKGFSICNGKTFKEVLLSRTNYYSDQEICEFLVSVLDTLSFVECEQGMYHGSINLSTIILLKNQQPNDIWYTYSMIDFGMFYWYDYNDEKVYFTRAIEQNDELWYMSPELITNYLNPKFQIAISPQKCDAYSLGMVGLQMATLGKMGIQIEQLLKDQEGDVERAVKRWIVNSKQQLTMKYPNTMNLVLQLINPDQMQRQFPSQIYQKHKNQQSYKETDVRINFDEALFHQIRQNTNPKITKDQMVRIFDGKNTQLNVNVKDKISIQEYLKQNIYKFEQHLKLHQLDQLEEEIEAFEKNMLQQFFYQLPYAKETGDIISCIDNYCNNQVYEILSSNIHEFKYMVLYLLSKVQMNILKQDYRYAQQLSLKSQEIIKGVEITCVHLLHDITKTEEERQKIQNVHLSIANREQLYELQVSKDQLIKNCKDDKNKLKDVKQLPYKLKQYGHFFQQALIYIYHLQQQYQLAHDISEEYIIDTSGEIVHDVKHSKKNQQGQFIIKEDILIKMVSRLKMYYMKVQISFDSWRDADFQQIHDLLNEINILHSYIKVDEENLGEIDKRKSSLVRQQLQNTQIKIKEDFQRSLDHTLQWLYRQGKFLQIMQLNRSKDNHGILKRLKPYTKNFTLQNCLLNQLNQIPQLGWTGEYFAEINDEKSNLNIFKTVTEIMEEVFFISESFGLKSQIFQNYNKQLLALLIYHCSHLLYYNPITISEGIFQGFMQILKDQTTELLNCILSIKILHILIHTQNHQYPYITKKESNNIQAITPAIMEQSLIKQLHTYYQPLLPFFFEEYNSKKNNNSWLKNNLMNNLITELIQGYFFINSKQNLESFEDEQLDTFLKSIEAKHASEESLCNKNGFMVVEHNLARARLCLLSQNHQQLPVILQTINTSMDQIAEEFVLQTDTKPLIQMILLSTIDIITIVQYQLGGNSFSQLCEFKQMPWIKKEKYESYYDIKFVILKILDLNLKNQQLEFASELLKFTLMLFENKSFLFNSTHSSFYQTLQIFNVKIFAYSDEIRLNILKEDKTDERQMEDLFEQVQKAQSFTKRTNNYLEVSKLLLSQSLEQNEMTQIKAALAQIDSASLENQVVLCQLGVKLVIYQMHTGQYSDADTTASNVSQKLFQLIYSQLCKVAIRWQSEKTFQKTTKKDLKIEEMFKILNQTSQIYEKYEHNVLALLEPIKELFLNVRQFKTLKQIIFLRTVFQYLESLHTLAIMMKGDFKLAQANLSFVDKFGQQSQVEEIITLNSNIQMNLDLIIADYMKGIQKSQLMIQTIEDKQQLLNVQIKTNKNERIFCNLEFEKYFEIKRPEQESTKQLVFSYDQTLMKSALRPYFEINRLKTEAFLCYLESSLNVADKLTQVVDPMFAIQKYVQQQYGSNHIFNAHIQRLIAGMYSEFLTTLRVKDLEEQLITDTETKINAFKAELNQKTIKILESKVKNKCSPAMEQKYLETKSFIQSKITVLEGINEGLSIDLVCRWSLIAANQSFAIYRNFCQEYSYEYSPQLAYLYSTYSDIYFIQSKIVSTMKYLEEAKSAYEFCQTSIYNPDYCKLTFRQGYLRQNIMNMIKNYVDVLMGLSLLTPSKKFEILTLILKEDKTYLQHFDNYKTHGFVRFLEQNLLKQLELDTLSGKRLPDLQRKDQKKLIQFLQVEGDKLAFQLTIDQKAKVPTHTIELIIKDGIETLQKEKIKMDYSGEEYFQKAYQICKALLPVDNPLCQKLEFYAVKQKSKAKRRTSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YI79</accession>
    <name>A0A8S1YI79_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1830003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000183">
        <property type="protein sequence ID" value="CAD8213640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YI79"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="87" mass="10331" checksum="9AB9643D1B8708C5" modified="2022-10-12" version="1">MNEIEEQYFTIKSLEDGYINLAKTTCVEEQKSNKMDRLIGGFEQQLINKLQSSIQKWIFIQGMLQFFFNFQISQKDIVPMDQDQPFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YJ22</accession>
    <name>A0A8S1YJ22_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1800015</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000180">
        <property type="protein sequence ID" value="CAD8213503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YJ22"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="35" mass="4297" checksum="45A4A823838DAEDE" modified="2022-10-12" version="1">MHPVPLVFLLLRLKVLYDLRNVCQYYKPCHKNLIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YPY7</accession>
    <name>A0A8S1YPY7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1910001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000191">
        <property type="protein sequence ID" value="CAD8213932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YPY7"/>
    <dbReference type="OrthoDB" id="307308at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="462" mass="53557" checksum="EF5355C0CDFE44B8" modified="2022-10-12" version="1">MSEAQYWTAEGVKTQNLIYDCAGSFLYGGYGIFGVGAKISKQLELPPHTKIKFNIDIWKLDDWYEEYIQIIIDEQIYKSQLLYYSQGSQICGLLETNENELIIPIEISISHTAKFLNIIILSTVNEDGFNESWGIRNLQIQILPCPNLCTICDQEPIQICSTILIQEIKINEWNNQRGFGILDKNSVIKSSVNLSAPHYQIKVKLQLILIDIQSLINYFVLEIDDLITNIQLTSNSQLSLCGSSYQESIKNIDVSINHTNNNSIIKIRPIQLTGQNAYWGIISFDIFIIKCSDKNCLKCTEKDVCLECIDEWVVENDKCNRLQNPFKIKQIKVKQYENILLDSQQQLKNESIILNKAQTFMDLTVTTQCNQNNYIQSNFIICHACEEKNIAYFQFKCQFKLNQMITFSIKLEKLNITSEELLWRSIIFSLILLLEIKMIKNLVQQQEIIQITKQIRYFIIMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RVC1</accession>
    <name>A0A8S1RVC1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0010406</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RVC1"/>
    <dbReference type="OrthoDB" id="292964at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006615">
        <property type="entry name" value="Pept_C19_DUSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF24">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06337">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51283">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="DUSP" evidence="9">
        <location>
            <begin position="124"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="8">
        <location>
            <begin position="424"/>
            <end position="1024"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8132028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1033" mass="120286" checksum="5A4356C6264C4368" modified="2022-10-12" version="1">MEKIQELVNSQKFQEAIEELTDLLESELPSPKLETLEVLNRILSLSTKRQDLQIAILRLAKFENTERYCDLILNLARLYIDLGQLSTAKQELEKLSSINNKYIKCILQGFDRMFNQRGEDIQKEDQWEQFQLLEKEEKELDYFSQDGLCSYLIPAGWFQKWRLYVKNEDPIMLDDPIMSLNVTKLRQLLNLNEDCINEETDVIPGPIDSSRLLQIKWNLLPDPTQQKQYLNYVLRNDLREGVDFVHVSGKVLKFFEKIYGAFVVKRLIVKQEEQERIIVDLIPRVVEIIILENQLEESSLQVNGFEQVQELTRKVRVIKGIGNEIRWWKFKDLQSQLDWMSMIQKGTFIKSIKGKELDIKFKIDDLNLTTQDILICEIKQGKSWQLTAETNAVQQKEQNVSQLYAKFGISNVPKALPDSRRGMTGLQNLGNTCFMNAALQCLSNTYEFTEYMVYNHFAQHLNPNNVLGPKGFLAASYAELMKSMWFANYSSVSAIDLKKVIGKFAPQFYGYGQQDSHEFLSYLLDGLHEDLNRVLNKPIVTDIEITNESDQQASEKFWINYTLRNQSRIQELMVGQYKSTLVCPICSRISKTFDPFMSLSLPIPSFSSFPFSLYFIYDDSENLPAKISLQLSTDMTGLDILKQLENLLNVSHKRMNMILIKDHQIQERVSYDKEAKWINEHNGILFVEEVESNIQNLKQNQINVDFYYVARAQKTNESDKILSFPRQVVIDRNLTSQQLYGLIYNKFRIHLTLAVKELTSKEFPLKDEFLTLPRPKSLEESIQEFLILVQNQLQPFTLRLKDQLNEKLKKEIQFNDNIILDIKGSNLSIEVIISDYLGKNAQILKLQRCKELTTNDTKSQIRKGEYDLDDCLRAFTKEEILGKGDEWYCNKCKKHVQASKKMEIYKVPQILIIHLKRFKTNRISNLGNFYFSNGTQKITSMVQFPHELNLNNFVLSKQAGDNQNYIYDLYGVDNHYGGIGGGHYTAYAFNSVLNKWIDYNDSNARTTSSNVVSEAAYLLFYRRRDSEKCQMFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RVL4</accession>
    <name>A0A8S1RVL4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0010405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RVL4"/>
    <dbReference type="OrthoDB" id="305975at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8132026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="123" mass="14427" checksum="29FF18AFF2466A27" modified="2022-10-12" version="1">MKKVYTVPTPREQKSIIQQKSQQDIIIKQGQFGYIPDQATKKEQSSKPSRPYLMFKPQLVEEVDENNMKKMKLSFHNSITDLQTFEELAQEQKPRESTPNLNQTPSEKTKTFVRKSMKHLTQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RYL2</accession>
    <name>A0A8S1RYL2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zinc-finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0010081</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RYL2"/>
    <dbReference type="OrthoDB" id="308391at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018866">
        <property type="entry name" value="Znf-4CXXC_R1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10497">
        <property type="entry name" value="zf-4CXXC_R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc-finger" evidence="8">
        <location>
            <begin position="125"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8131484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="311" mass="37496" checksum="FBB87499563FFD31" modified="2022-10-12" version="1">MHTQNNFMYNSQGYMYPQQYMYPHPHPPPNYVFPQQIQPPQFYYMNPQNPAMYQYNIGQQQQQQQRIKCNQPKTKEQELKQQKQQVVKQQSNQNIQQQIEMQKTNLKILTEQQIRDQVGSITKQGFCHYCNKNGKMCYYCFSNYRSTDCRHGFCFDCLMFKFKINPIKILLRPDWSCPIKCKQCNCSRCTSLNQMDSDQFISNDSVESCNKYAHIIENTKKIKKQYQKKKNIIREMFQVKFQQLDPKKKKQQQSMKKTLLLNKIKKQMQFSQECILKLKQKHIQNDEQILHQVVRYNFSSLIDLFKEYKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RZV1</accession>
    <name>A0A8S1RZV1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0020455</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RZV1"/>
    <dbReference type="OrthoDB" id="292811at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398:SF0">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="397"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="397"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8133538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="567" mass="67153" checksum="D4C6A262BF34C6C9" modified="2022-10-12" version="1">MQRRRLFIKGVKKFDLFAQPIQLLVNEKEYHQTIFGSILTLGLLLLFSNLLYSKLISLFDRNNPTSLSSEIYHSQPEIYQLLPQNFTMTFAFQNSTYHTYIDDSIYVVKAQLVHKYEKVVDGNKNEVWNKQELPLIECSSDIIKQKDLSEYFSHFDLPTNYCIDWNKIDKINLEGTFDAPAYQYILIQFFICTEETKKSKQCKEQQEIKKALEQNYFSFQISSYTTNLMNPNSPYNPKGQDLFTTISSNIYKEISIYLEPLTTLTDVGLIETELEYYKTLRYGRHTEMLDISSNDLIMNVVVRLDTTEYVDYRTYSKIQEILAELGGLWQVLFSFFYIISKPINRISRYLQLINSLFELNDSEDQDENICYQQYSLLRQAQDISPMSIQIPQIETKTQQKDGLKNKLKQEEPKKRKESSVKPQKTILTTYAEYLQRVIIRKKKKLEFGYFEAFQKLKCIITQDNERIKQFQIAQKQIMSQLNIVEILRKLRDVEKLKYIIFNENQRLLFDNFPSIQYQQIEDKHDRFDQQHKLSAQDAILQIIGDQNNEMNQKLLASLDQTHDHPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S184</accession>
    <name>A0A8S1S184_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0030055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8133946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S184"/>
    <dbReference type="OrthoDB" id="297263at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="238"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8133946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="649" mass="76446" checksum="73C087A27A125418" modified="2022-10-12" version="1">MKTKIVSLFLKREHLILLSKMLQLTENTNEQDIQIQKIDSQNQKSENRILKTERWPQQDLDYFQNSFLQIANLTFSFFKFHFPANSALQIKEHIKFIHVVKQINFGGVTIEEVFKQQRTRLNIEVAKITKSIKLIDEPIMPKLMNSKIDLAYIQTIQDKYFFPKNNDFIAPYLILNLKPHIPKTTIRYQNKELQQSITSLEDIPPFQFMNSNLIQPILNYESLKNGFDYKFNKLMQRNQLVQCTIKRYSNKIKQYEIKSEDANIEDSLQEIKKLFYAQIKQLCDNKITSTLIDSNLKFIQPQNIEESSCKFTPIQTIKDFESLLFNQITEQQESQDIIHHHNFNEQQCIRLHQDFIQTPNLLSINKSQKFLQDSAIKQVETQKNLQLSENESTIKLETRLIKPSLKSLDLKQFELLNQSTSRHQRKMYNQKIHEQNDKSQSNHKFFNIPLQTIEPPASQTEQQNIDIQKSIFTNRNLQQKSLDRITLKPIAYQTVRMKEIRDSNKNKKEASFPYIKESLPIIGLSVNDTEQQKLKECESKFSLQGSLSKRVQTNEKEENSVKEILLFQKKNCNLKNQLQHQLILLTRSEQILTTLMSNGQIRGTELYQQLLQPIKINSSAKFLTPKSNNLMSRSKFKKIQQESKMFSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S1P8</accession>
    <name>A0A8S1S1P8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">H(+)-exporting diphosphatase</fullName>
            <ecNumber evidence="2">7.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0020437</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.3.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S1P8"/>
    <dbReference type="OrthoDB" id="5210at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009678">
        <property type="term" value="F:diphosphate hydrolysis-driven proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01129">
        <property type="entry name" value="PPase_energized_pump"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004131">
        <property type="entry name" value="PPase-energised_H-pump"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001960">
        <property type="entry name" value="PRK00733.3-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01104">
        <property type="entry name" value="V_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31998">
        <property type="entry name" value="K(+)-INSENSITIVE PYROPHOSPHATE-ENERGIZED PROTON PUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03030">
        <property type="entry name" value="H_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001265">
        <property type="entry name" value="H+-PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="98"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="144"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="285"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="359"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="404"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="435"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="500"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="538"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="610"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8133502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="738" mass="78618" checksum="DCC2A20FC5F95C58" modified="2022-10-12" version="1">MISFELSNILILVSTGIGLLWALYNAYKLNQIKIGTPNQYNNFQDDYQDHHSQLLLEIASHIESGAAAFLAAEYRYIGVVIILLALLIFFIVEPVLGQAWTTVAFLVGALTSIISGFIGMRVATFSNYRCAYCAQTKMTDAFAVAYRAGCVMGFALVSFALLSLTILMGIYINWFIKDYRDFQQLFEAIAGYGLGGSVIALFGRVGGGIYTKAADVGADLVGKVENNFKEDSPNNPATIADNVGDNVGDIAGMGADLFGSFAEATCAALVVCSVSPSFYYHPTTFYYPLLVSASGILVCFIVSIFAFVGQKEDFDQVSNALKVQLILSTLLMLPALYYVAYLTLPERIFGLSPVDRQPIHAWLCTVVGLISGCIIGFVTEYYTSHSYRPVQEIAQACGTGAATNIIYGIALGNLSTIIPVFLLAFTAFISYSLLSMFGVALSALGMLSTLTIGLAIDAYGPVSDNAGGIAEMVGYPQDVRHRTDQLDAAGNCTAAIGKGFAIGSAALVAFSLYGAFITRASNSLNKHPLTDLGVNSPLIFLGLLIGAMIPYWFSAFTLKSVGRAAFEMVEEVRKQLAEKPGIRDGTEKPDYDRCIAISTKSSLQEMFAPGLLVILVPLILGLFLGPTAVAGLLPGILVSGVCMATSSANSGGAWDNAKKYIEADLCEIDDIIRGKGTDEHKAAVIGDTVGDPLKDTSGPSLNILIKLSAIFSLVFAGVYDKSAWLLCAMTTSSSGCPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S4Z5</accession>
    <name>A0A8S1S4Z5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0030095</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S4Z5"/>
    <dbReference type="OrthoDB" id="289215at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050391">
        <property type="entry name" value="Mito_Metabolite_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45618">
        <property type="entry name" value="MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="205"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="243"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8134024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="339" mass="39605" checksum="3FB2A1840C63E826" modified="2022-10-12" version="1">MYQCIVFPADYTPFKQELILTGSIMAYELLTYPIDFIGNRSIQRETYLSKSLIQESSLIYSEVNHYLIFYDCWFKVRLDLSLSKIVLRYYIFNYLYFKYAFKNYISPRFNDEDESKGYDITPENSTVYPLLRYAKMTDPLVLLIAATSNLIGMIASHPISILKVRLQNEILKQHETKTYTQKMTEMSRMCGSQIWIKTTFGSRFLYTYLQFVAEMGLFQFCLNKLGYEFMNVFRPNSDFKGNIISLGFSVIGSSLVSTLLLQPIYIYQQRTVYQVITKQPKKSIKSFFIQLYQKEKAQGFFKGYLHNALRSISRNGIAVGTLWGLATTANKERFRPSED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S777</accession>
    <name>A0A8S1S777_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">S-adenosylmethionine synthase</fullName>
            <ecNumber evidence="11">2.5.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPENT_87.1.T0040300</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the formation of S-adenosylmethionine from methionine and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-methionine + ATP + H2O = S-adenosyl-L-methionine + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="11">Binds 1 potassium ion per subunit. The potassium ion interacts primarily with the substrate.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 2 magnesium ions per subunit. The magnesium ions interact primarily with the substrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the AdoMet synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.6" evidence="11"/>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S777"/>
    <dbReference type="OrthoDB" id="5852090at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18079">
        <property type="entry name" value="S-AdoMet_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000003">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000004">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000011">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022631">
        <property type="entry name" value="ADOMET_SYNTHASE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022630">
        <property type="entry name" value="S-AdoMet_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022629">
        <property type="entry name" value="S-AdoMet_synt_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022628">
        <property type="entry name" value="S-AdoMet_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002133">
        <property type="entry name" value="S-AdoMet_synthetase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01034">
        <property type="entry name" value="metK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11964">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02773">
        <property type="entry name" value="S-AdoMet_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02772">
        <property type="entry name" value="S-AdoMet_synt_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00438">
        <property type="entry name" value="S-AdoMet_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000497">
        <property type="entry name" value="MAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00376">
        <property type="entry name" value="ADOMET_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00377">
        <property type="entry name" value="ADOMET_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 11" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="10 11" id="KW-0630">Potassium</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S-adenosylmethionine synthetase N-terminal" evidence="13">
        <location>
            <begin position="23"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosylmethionine synthetase central" evidence="14">
        <location>
            <begin position="134"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosylmethionine synthetase C-terminal" evidence="15">
        <location>
            <begin position="257"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8135585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="419" mass="46696" checksum="FB06B2C7EA9EEEE8" modified="2022-10-12" version="1">MQQVDNNQESPHKKKFVLPKGHFLFTSESVSAGHPDKLCDFISDSVLDACLEQDPNSKVACESACKNSLVMVFGEITTHAQVPYETIVREAIKSVGYDNINKGLDYRNASVIVSLDQQSQEINQAVVGSKHEDEIGAGDQGLMIGYASDETPELMPLTHHLCNKLISRLQECRVNEICPWMRPDAKVQVTVEYKREDSSFYPVRIHNVLISQQHDEKITHNEIEAELHKHVLKYVLPSKYVDEQTQYHLNPSKAFTVGGPYGDAGLTGRKIIVDTYGGWGGHGGGAFSGKDPTKVDRSAAYAARWVAKSLVASKLCKRVMIQVAYGIGISEPLSICVNSYGTQAEGLDDDDLSEIVQNHFDLRPGVIIRELKLRRPIYAKTASGGHFGRNEPEFTWEHPKIINLSAWKAHKAEQQQQNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SCG6</accession>
    <name>A0A8S1SCG6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WD domain, G-beta repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0060192</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SCG6"/>
    <dbReference type="OrthoDB" id="284161at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF8">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8137638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="663" mass="76680" checksum="D58FECCC29ABE55C" modified="2022-10-12" version="1">MEQNISIKCPIHRRELIVTGCINPSCNRSPLICQMCPDAHSEHYSNIVQISEWLQKVSKTIQEGSKTLKQIRDINQIDSVLKWCEDNEMHLKKVDEHCKAQKALINEDFNYLKDLLVEKCNQAQAQLEQDIEDFYTKYKEQFKICKLIMDDSYILQKQCNPYSNVHNLIFKLQQSNSKEAQELINQIRKLINKPYDPIENIKQCAESITEMTYNLPVFAFQDGIDSFVNQLADQIHNHFKTKLNITLPRKIVSPIKYQPKNLHKQYSLPTENMANNYNSNQNISKSPRLNQKLTFSPQNSIALHSKTIVGQCSDFTLKPLKKYQLDFCVSAIEVVTPQIFAMTCSNDPHLRVFDTIQKRIHTLSTHTTPIIQIHKSIDQIVPLQQNNNSKPQTYLFTLSNEQLVVWAFDFTNSQMIVPHIYHKYLFNNQITQSCYLQDNSCLVLGDQVGTVEVYNFQQSKLQQNGIKGKHQKQISSILLLKKHDKFICSSYDQTLSIWKMLYNQQSFENTYCESIIQCGNMLGQIQIINQFQSNPNLLLVGNLEGSLKIVNLMNQCVILESDIQKNSNSICDFIIAEDANKETISVITYLQSSKSLRFWRLQNNDMGWAQNEQKIEVALSLSENMIRSKLQLVQMPSSTQNTVVLIANEINKELLVYEIINKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SG69</accession>
    <name>A0A8S1SG69_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0080062</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SG69"/>
    <dbReference type="OrthoDB" id="302072at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8139365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="136" mass="16237" checksum="4A8117B877BEA4D6" modified="2022-10-12" version="1">MSINNVSWEQVENPSKTKFKQLYLNKYRIKLGLFSNHKEQLPISRPKHRNISIKELDHLIKPEMIKAKIMPDNQNQKVLTAQLLNKITQKQLEQEYFCQFPEITQTKKKRSKTEHHNNQYSKLQKSFARTAYLIVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SKH2</accession>
    <name>A0A8S1SKH2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0090014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="294250at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1520"/>
            <end position="1543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8140320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1597" mass="189929" checksum="B5EF0EE9BAEF6415" modified="2022-10-12" version="1">MLLIILVIQIAYAQIRNQTSTLIMQTMHESYHYDLLTKYSDYLNPEFQIINIAPMNNFTKEKAQPNYTITELSQFINSIQFNKSYQTTSQYNLKKYPITIFLCDKDVIILEYTKNINNQMDIYSHHEYDLTNTINDLQSCTTMYGLFDDLKVNIDCVDQQNNFINVILEIILNRNMKIQDVQFTIQFYSFENILGSSEQVTTYWNNCKKKDIKYIVHFSQISRIFYCLYGNLIQPQDQIILIEESTQLIDDIYLIKVYVLDSHNFNKCNSEEIVNYFYIKQLIANSITRITEYTQYEQQNKWVLSLLLVDRHGICKCGNLIKNKISCDFLELQEGLYTNLYSSSYLEEITYIYVASEILLYKINFYFTYLTDQDKIEGYFYIKQDGFNLQKIHIYYDYLIVVMTKKNNITNNDNYLPQKILFFQKKYNIQQYVPNYIIDTSTNYGIFRVNFDEFPGSYYLEYNYKMQNLSLHQLQCKHLIFTYEQEIKNKNYLMFQITLQITNLTISAIDIFNLVISQFNDDSFFLIKENVQDDLIFKSEQFNFNLNTNLLGPLLNYHEIEWEDNPVTASSVIEYIREIKFMHFKSEFIKVFSGLFVYNEINAYFSLVGLIKDESNETILAYQQFKYDKIRFVEEGKLKQIKLEQFQFTNIDFFSLNILTAIIGDSAQKKYVLCYNNQFSSCTQLTGDFLIEQDTYDYIQLNEIRCINDTLFAIPINRKKIILFSLTCKIFFVSQIDIQKMGQIDYQTIPELDEFIILDARFEIIDNKLSFLLLSDGSIMQIITYYSISDYKLNYKVINKIKEPDCIPMMKNSSKIFFQFHQDKRVLVYICNQDSQNQKIFEYDLQNPYNPFLIRRYSLFSYQINNLLKPFSDSQVIYIPVEAKGYLAYSPFFPSPNILLHDIPVSKDLISAIPVIVNNFDTNEWDIFSMAFFFDNNGGTLFYLNSNPVYYSVLNNDDTESKKMIMVYQQNQDSIIFKEFDIFFDYSETQIIFNPNNINLVVNSQNVNLDLNNSEVQGAVEDFEIQNLSDQKNIIIKKNDILQLNQYLSDHYETLEFGRIFDILDMNQTEYLSIINSTAYENNLIFTVSQYYFIIYEKQRQTTKHINRITILTKERLTNLNSQLENLRQCRLSINDINQVLLYCYKYYSSSLQNHSKLVQYFQINFQYDYANSKISYKIKPLKISLGNRLQIEAINPIKFQGQIFQFIKYNYENSQDQYELISGKIENGQFTSKQIIQSSTFDYNILGQLSFDTILLNDETILVLTLDIHELRVLFFRLDNTTFELFKTKEEKFSYQQLNQYLFHSINQYRLETIHLLDCQDYTCLVLLGTPDKFFEFHLILSETEMSKIVKKYIYLNYFRCIHTNKQKPQIQLKSNLQISQLVAACVKQDVEKNFFFEDKFDYLYDDLQVFVQIFHKIQDANESSPIRIYRFFFDITLRDRFHFLLYIREPDNNIHILFENDTYFLIDYLKQDKISFQIKTNYTKTTTVTGNLILSNIFKKKTVDISIKFEFPIERHYIWIYITAGTFLLIILIVLGILLWLKKRRRQKCRKRKNDSFSKSLIGLDDVLKEVILGEGKRGSQTKLDDSYSNENDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SNA1</accession>
    <name>A0A8S1SNA1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Arrestin C-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0090346</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SNA1"/>
    <dbReference type="OrthoDB" id="296266at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF17">
        <property type="entry name" value="FI21816P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="1">
        <location>
            <begin position="150"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8140916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="318" mass="37301" checksum="F82B613F2484A2C4" modified="2022-10-12" version="1">MIPEKLLEKNKKVQITLDTPRNFKMSQINPKKKYELQNGTMFQITRQYGVNEDYIHIQDLYRGIIKSGDYKLPFQVPTKFGMNSSFQYRNSDGLQQAKIQYKIYLKISDYQSNQVIIQKSIPVYINSRFKLVEQKRESEGNIVQFFCVHRGMIELSIRTAKNLYKPGEVLELEYILNTTRAQRSITKVEVKLNHYLSFTDDDENERVIENNILYTHELPGITAGKRSDILKHSITIPNDLTATVKMQFIKNHYILQVQAFSNGLLTSLAIPVICQIPIIILERRKQDKPNLDDWKFNIQSQDNNSFYQFTSQQQQLFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SR01</accession>
    <name>A0A8S1SR01_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0120092</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SR01"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="33"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="144"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8143205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="200" mass="24216" checksum="39CFF44091F7B423" modified="2022-10-12" version="1">MYDEADGEQFEITPSDDGLEDVEVIVNKEKQYQQELLQVLKQKEDEHKTLRQELEDIVCKIQQIQLKQYQEERPMVDPYLREYMQELGEQQLFDLESQRQELKGEKQGKIEQLRQLQQAKNQIKLFFTQTEISSYLQISYKNMLENLNQELFQYMQERADLEDEQFGFDKRLKSLRSQIREVTEKNQKIIQQIENNKQIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SSX1</accession>
    <name>A0A8S1SSX1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0120499</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SSX1"/>
    <dbReference type="OrthoDB" id="298777at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8143975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="788" mass="92802" checksum="26589B9DD4F59397" modified="2022-10-12" version="1">MCSECQFEHQKQTSGDQIVPINILIKMMEEKIQQYHLNDHNYIMNKEKNFFTKIEEIKQGLDLILRNSSILIEEIKKEDNWYLDLLQQNPLECTQENINNIAYIFYNSKELQRRQNQKDLIVKIYESKLNLVCKYFKRLMVILGDQIYQNKKQAFDNKILQGPLLLGLKHGEYFQIGAMKFDEKAGLWKDEGKQLFRSIQNLKWLFQTTQFQYEIKSQGQIIYLKDGTILGEENRDKMVPSLNNVFINRNLEQVKHLSWIGQYKQNYQKQGKWIPIWRGENLEAGGTYNVEGIKEGQWKELSSNYCDFCKVFYVGEYIKGKKVGKWNINFEEQIIEGGFYNQIEMKQGKWTELGDSFWNQSQIFFIGEYKKGLKVGNWNINYRYHKLYDQKRIGGGYFSQNGEKTGEWIELHPNFFVSKISSCQVTFHGTYQNGIKIGEWLAIFQEFSNSEERIIGRGFYNKNGLKIGKWIDVVDKFWIFCQVTEVGEYNEGRRIKNWEIQTSKFKNENNSKIGGGSYQEDAGLKIGQWSELHENFFENCDVIYSGEYKNGIRVGKWIIKQKQKNLEKYKKIGGGTYNQDGQKVGKWIDLFENFSEYSIKQSSEYQVFFIGEYQMGRKIGRWNISVVQLNNTHLENYGGGFYNERGLKVGTWVELHETNCANRYSENSFNIIYAGVYQEGKKIGRWDTKYREEEQDCFQLMQGKSQTIEEAVVIIKMEQKQGSGLSQILILKINKAEIGFQRVNMNQEQEKDHLQREIAQSKMIDCQEIYLFLNLQLIQPSVGNISHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SV58</accession>
    <name>A0A8S1SV58_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0140300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SV58"/>
    <dbReference type="OrthoDB" id="304322at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="64"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8145551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="340" mass="40825" checksum="9216F405139DB396" modified="2022-10-12" version="1">MYRIPLDDYNSNLDTKFNTCSSTRNSNQPKFNFSTDRTSRAMSGRSCNVKNQFDVMCFLMGVELERLNQENDKLKYQNLEIKEKTYDNKNYELQLKDISERLIKKELQCQQLENQLTDANKMIAQKEVLVQNQYQQMNQQLQVIQQLQYTIKQLECKLGFLVCENENLNTKRNDELINQQQITKSQKDYEELQKVYSSLLDNYNLQNEKLKRIDVQHKELEQNYQQVLEKKQQQEMENKNKMIQYVDCKQKLTLLTIELDRMNEVIQQQKCELDQKQKKSDNKQQILMMRIILHCAEIDRLIEVQSKLMNENKWYRNQVDLYAMMIGDNNNNNKVVKLDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SVK1</accession>
    <name>A0A8S1SVK1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0120218</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SVK1"/>
    <dbReference type="OrthoDB" id="298345at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="134"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8143416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="323" mass="38337" checksum="7508AC538811847B" modified="2022-10-12" version="1">MNTHQIQYLYKPPTSPQQLTSRMTINDQISPIRDIIMPKTNRVSQNITRNSARGIPQLITTKTQPSVARESCQITRESIHIKQPSSMPIDNFTKAIYQLSQIKQKYDKSLESQIDAIIIMLKTSNFDKENCNPNQELKQQIIQLQSSNEKLQNELLHKKCEKTNIQNLEQIIVNLESQNKSITNQRDILKTENEKLQLKLLNKEQHFVSLQQEIQQQRNSFQPYELLYNQQRQKIIELEELIQSFKQINCTENVIKQQDYQDLKWKFEQSIIAYQRKDKDLQSKVEEIKKQKYQVEQLLKRNQTLEIKLLEIMETEVKQRIIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SW39</accession>
    <name>A0A8S1SW39_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0110274</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SW39"/>
    <dbReference type="OrthoDB" id="296048at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="74"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8142662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="224" mass="26382" checksum="D4AD3D9A52E3A3E6" modified="2022-10-12" version="1">MNMNDSFFDKIEVVNQQLITYSKPQTICKSLERSFAMCSNPPNKIIQQQTQFHNNDTAISRNQSYKLDYFLKQQTKSSQNNTQSKSYRSIRQQDYNTNNISQNPQKSYLKQQMRLKINLENDAYQSNLRHKTSSSHNTTNPYQASLANLMKQQMQKQQQSFANICKTLNDESKPRIPSTHRLSIIPQKLTQRQDSVNIRKSNSVYMQEFRKVFSKSKYSFHNNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SXD1</accession>
    <name>A0A8S1SXD1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0120212</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SXD1"/>
    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000285">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051964">
        <property type="entry name" value="Chaperone_stress_response"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44029">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44029:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="5"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8143404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="246" mass="29221" checksum="3487472CC6517513" modified="2022-10-12" version="1">MDKQDYYQVLGVEKNATTEEIKSAYRKLALKWHPDKNPDNQETAKKQFQLILQAYTVLCDSQKRANYDKFGTADGEDQMNFDFDDFFAQDFESMMNFMMGDAFMKMFTNIGRPGRGKHKFKIPTSFVFKQAQTQKKPEKKKEEEDEWETEEEVDEDDDWKDVDDDDEDDKNDNDDELQSDSDDDLTQTENLFMMPMFIEDSIKDTDSNKFKCKFDGQILKEQTLVQHFEKNHKKEFAAWSKKKHFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SY76</accession>
    <name>A0A8S1SY76_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0120342</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SY76"/>
    <dbReference type="OrthoDB" id="293577at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8143664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="610" mass="73461" checksum="EA01478E5D9102C2" modified="2022-10-12" version="1">MNTKITLRNSKQNSFLSSFKTTHRHTLSQGIELKMESPTNRETSGFTSFQSFKSSSMHLKKLVKSPNLSLKSARLYDLNDNFLNKRTSIVQMMQTFSNNYDIIQQSEPLENIDLQKLFPVEEQKKLQNNEIVSDISKLRIFELKQENNDWIQINRFRFHYFSIKIQGQESPIQVYVKCDQLRLLNYRMYISTTASFPTKFNCEAIIQSKYFKYQDKKHNDKFLDKFLYIAIYSEEDCEISISFLYGNKFIKKQKPKREKPNYDKYKYWWKQSKFDMSYETDKINSNLDTRQYSREARKEKLIQSQDQLDLRLKNAITKKKELCEDKKMDLLSKYQANEALKEIRNQQKLKQRIRQALIKFQISWFDVFLLIHLAENMKAMLDEAKKRSKYLAKGRLIVWKYRTTTMIKIKESGQTVDQRNLFKLCMAMCMYTFHNKKRIKTEVAKVLRYFFTLTHQYCHFIQKHQELTNKVNYVKRVFRNLKQKEKAYKDRLWRILNKYAQEILHSLGAHFSNPKKRTYIIDSSALMKATEDFLISKKRKCQKFIQSYVREKDEKQKVIDLALNMMQPEMFDTPSYDDLYGIFRTYIQYKKITSQHSQQQQIKSQQQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T0W4</accession>
    <name>A0A8S1T0W4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">methylcrotonoyl-CoA carboxylase</fullName>
            <ecNumber evidence="3">6.4.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">3-methylcrotonyl-CoA carboxylase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">3-methylcrotonyl-CoA:carbon dioxide ligase subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0140087</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>3-methylbut-2-enoyl-CoA + hydrogencarbonate + ATP = 3-methyl-(2E)-glutaconyl-CoA + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13589"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57344"/>
            <dbReference type="ChEBI" id="CHEBI:57346"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.4.1.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid degradation; L-leucine degradation; (S)-3-hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AccD/PCCB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.4.1.4" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T0W4"/>
    <dbReference type="OrthoDB" id="439921at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905202">
        <property type="term" value="C:methylcrotonoyl-CoA carboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004485">
        <property type="term" value="F:methylcrotonoyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006552">
        <property type="term" value="P:L-leucine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000046">
        <property type="entry name" value="Geranyl-CoA carboxylase beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000004">
        <property type="entry name" value="Methylcrotonoyl-CoA carboxylase beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045190">
        <property type="entry name" value="MCCB/AccD1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22855">
        <property type="entry name" value="ACETYL, PROPIONYL, PYRUVATE, AND GLUTACONYL CARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22855:SF13">
        <property type="entry name" value="METHYLCROTONOYL-COA CARBOXYLASE BETA CHAIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="7">
        <location>
            <begin position="34"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="8">
        <location>
            <begin position="288"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00026116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00052347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8145127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="546" mass="60420" checksum="C319E729EDE1A16D" modified="2022-10-12" version="1">MMHYRSLFRFSHYPVLQSLCDRADPNLQANFELMKQSNERFLSITNKVINYGGTVAHQKLAERNKFPVRERITRLLDVGSPFLELSQLAGYELYEKEEVPSGGIVTGIGLINKRFCMIVANDPTIKGGTYYPITVKKHVRAQEIAWENKLPCVYLVDSGGANLMRQDEVFPDRDHFGRIFYNQANMSAQGIPQISIVLGSCTAGGAYVPAMSDENVIVSGNGTIFLGGPPLVKAATGEIVTAEDLGGARVHCFTSGLTDHFCTSELEALQKGRSIIKNLTTKQIGNIRDDQPIYDIEDLNYLMSSDLKKSMDSRHLIARILDGSRFMEFKENYGTTLITGFGELYGQEVGIIANNGILFSESALKGAHFVSLCQQRGVPLIFLQNITGFMVGRKYETEGIAKHGAKMVNAVATATVPKLTLLFGGSFGAGNYGMCGRAYGAKFLFSWPSSRISVMGGDQAAGVLTSVQQQTIVRNGGEWNEKVEKDLKQKYSQKYDNESSAYYATARLWDDGIILPTQTRQTLGLALLTSMQHYKYERTGHGVFRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T276</accession>
    <name>A0A8S1T276_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0140320</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T276"/>
    <dbReference type="OrthoDB" id="296756at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398:SF0">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="621"/>
            <end position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8145589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="662" mass="76447" checksum="F25E6D1604B8A5C3" modified="2022-10-12" version="1">MLKFQKKSKNEVKTCILDVVNSLDFFGQPPFFRILKRQKFNTILGHFLTVILMIMCALYLYFQFQDLLDQRTPNIIISETQPFNTPAFSLNSENFTFAISITGPSLSSLTTYKKHFDIAMTACNRTRFYNETTQTTDISLICRNIPLESCDLETHFPSEYQKQYFGKFRLKNMFCINKELRQENPPQLQGYTSADTYQYINISMTPCKNTSTYDGCSPVEEIQAGLKTGFFALYLGDTLLKLDNPGRPYEEIVTVQFAAFSSSLSKQIYSNFKMIETKTDVGLIQQDFVTELGLIQSSIKDSSGPFNQNSYVENILFMDQRANNYKRSYIKVQNILGNVGGLWQLVALTITTLVSPIIATLMNLQMANRIFNFENVGEKVKEGNSISNSNQNINIKVNGKPEKNDLIVELSKQGRQPQSSKRDLEKVQNRGQLKQYLKQRKRQLKVGLWDLMCMNIGLKRKHKQQIDYAIEKILCKLDVVNILTKIQEIDKLKYVLLNKDQLELFNYIPKPLIPMDMFSQDFEKKLNVLEEKAEFQFILQEEKSDLIKVESAFNAYMKLQEKKRLSDTDKSILELVGDDMAAIFDKINKNQDEFLLQSNRYLHSNLNLLEQNQIEILSPISSKSDNDQYNKSENNFDNENYIGNEDNEDQVAQIPPKIPSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T3T7</accession>
    <name>A0A8S1T3T7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0160213</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T3T7"/>
    <dbReference type="OrthoDB" id="300222at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8147110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="523" mass="61863" checksum="7ABB8872A504E99E" modified="2022-10-12" version="1">MFNKYFTFYKVCREKTKLYKFIKNVYIEKIKQIEESLLYANDLQYTILALENTKLINSDNTKQIVEISKQIFHNLNISKLDTPQKAVDLVKVLNENLSANDVRLYREIYKFYKKHAQYFNHQQVETLQKCYYHFARQLNFNDLIQIDEKTSSAKHTENFNYIHSDIYQLKQQEDWNIMIKDDPKSLTSAAILTHKNGIILRIQGVYQNNYESIIDTKKLISEKKPDIIILNMAPIAINENQNDSELNIQKTVDSINQTEKEEEKIIEQVFVTNFDPLIQQLTKKLQINDQKKILTGYQQGYQVPFTMESLLYQFSLQKRDSKQPIILLGGLNLEDQIRLYLKNIPSTKITQELSSLRYQWLNQLLNNFADIQQNGCILCNSQPNGLMPPSKVLNEQQRMKQIQADFLAEITNKYIGSSGKKQFLVIVQQQLFLETLRSIAQRNIDKNDVDIDKYREEYFEKKLGKKFLNQKHYKVFQQFYEDALNCDTPFKYCQQEMLDLNKFSSEKYKKGLDLRCGIRDILF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T592</accession>
    <name>A0A8S1T592_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0160031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8146746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="291722at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035715091" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="1633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8146746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1633" mass="190628" checksum="A54A112D8CF3A924" modified="2022-10-12" version="1">MSIMRSMFILILIGYVQSVDICVQQTTYEQCINLKGEQCKWNGQYCRWSNNYLFGCDPTLNEKLCTKQVGKLDGTIAMCIFDKTCHTIYEQYFLKCSDKLSKSACLSITNPDQLCKWEDDQCINLNQTEFNNINAIFQNLELSASVCSRITGFLIIHHSILWKLLEYSPDIVTEAQRKVEIEAGVEFQESKTYQDSNLKDNVIDSNGMFIWYTLSTKQSISDTKLNDFQRYGCIAQEILIENRFIKLFSLSGTIRGVNHVYCKYLNVHPTNAIFTVFAKFECLPTTEDQLQDESYINKNQLQCQDMSGIVCQRFKSSEIKCVIDYTQEFQCRNTDIIVEIDECKTLSGIATHYNCAIVIQQYCYLDISNSKGQCSQQCSDLTSKSQCENNQQCKWLGDNLGNINDSKLSIFRVCVPLKGCNQIGMNKKYCQEMSMNCYWNNIIQKCQEIKFIEQLRCEDCNSIFCCSSITIYDQFCIWNVDKCINVKDQNLFSHYQSFSSSIVMNQNLCLSQKGQYNYYDIVTRTCKTQNINLYLPCVDFQDKDYELDNKPCDNGINGRINQELCLALINSNTKWDASLQRCIHVTNKSISCDNMQFVNPNLCKYGIVQESYLCLYNLDTSSCYSSQLIDLHCDTPGINYEQCISNQNENCQWKENKCISLTVNLIVQYYCYEFQNVSPNVCRFTSRNFSCEYDHNTFGCKIITNQIINSHNYVNNQGCLTSTGLYYFDNVQNQCLDATDYLNILNCDTEFISIQTCLSITTQNQNCFWGSIPSDPQVKCRQYKQQFQTTCYSYQQSANLNTCTQMASNLSLILTDDQFCEVINSKCVSSISKIEDIDCGYNRMINIHRCVAFTQKNCYFQNNRCNLLFGAQDYQQKLLGILECQQSNLQICIQILTSGQTCHITQDNKCVQLYLSFSDTCQNISQYDMKNYNPQICSRAFNACYYDVESKTCKEPYNLKQFKCNQIGASKKLCLLFTYENCVFYNEMCQPLVDLNIDCKFRNRNACFQGIITCYWNEMNSACQPQYIDCPFYYDKSISWQICQSSNQMCFAGQNGCVNSFHEVPCWLSLSQFLCNLQWKYCQWLDNQCVNENYDCEQAQTQQQCLLHRSNFCVFHNNKCFTILNIELYDCDQFDQVSENFCRQYQPICTYSQTDFKCIKMNFLTNSQMIDEQFKQNTCSSINQNIYSCILQRSLKCSYYNYSWYQFCANSEELQTCNYYNEMIYPSILTCESVSNCKFGMTTKGEPFCSTSPLRCENLQYFCLKDILGLHCYSEFGSDECLTYNDELFCENVQSFRVNKQLCLSIMKNPNNLSECYYQEETQTCKLKNPLSIIISTEILYDYYYCLFQDTELILYYDDNQIFEYCSTSQEGKRENLNLYGCTQLLDSTYYFDLEQFKCYKDITEPYIQIFLCHQMNELACLQIQNHLSCVWFNQKCHKYKNEFINVPCQNRNYNSCLKSQTQKCIWNQDILQCKDSECQNNQYVINQAQIKIYVQIILKVQLVIGMDTTVKKQIFIYLIVRMKYHNKLVFKLKNQNKFVNGLTKNVKIYINQINAMISLIYNIVDKHLLVVNSIYHYKNVNKNQITLIYVLTHQVIKLAKMLNKNIVNLMAIVIYGIGQDILVKTYMPQNSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T910</accession>
    <name>A0A8S1T910_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0170052</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T910"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="93"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="5">
        <location>
            <begin position="380"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="6">
        <location>
            <begin position="759"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="350"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="814"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8147614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="887" mass="103192" checksum="26D98DD1228A0172" modified="2022-10-12" version="1">MKIGAQQKFIICNIIASLLILILGITQIIAISNSGLLLILDLLFSIIFLILEWKQKFISQTSDYYLGVTQIIMIIIEIEMLKEIAHEGSFIYIILKLFALIQILQIQILQSLIFFIGLTYLIVRFNEFQYAISYIGIAFAPIIIIYEREDANDLLELLRETLPASILVIDKQTKKPLYHTKNLETEFKYEILHSDEFINCLNSFYSENKIKLQCIIDSFIPQIYSARQNFKNLFSEIMDNCQYLSQSPLFKRFSGQFESPEDELNEEREHKHDNEIIIETPKNKQYSKPSILLSEETPKHQKSKILEELIIKSQSQKTICLVQLRKKKLKINLNHCLWYRRQAFMIILQSKEIDKQLENLQEQLNQQQQISENKDLILATVFHDFKTPINGIVSILESLDCKSEITQIEKYYHNIIKKNVYLMLYMIYDIQDYARIQKKQLRLCLTDFYINEIIDEVIEMCSIQAEQKGVEIKTYYDIPSYQIHSDPNRIKQIIMNFLSNSLKFTEQGNITITVSSLNSDKSQHIKRSNTSKNIQYQVGDQTIQQIRKTLQGRFQESSINKLVYNISIEDTGCGIPDNIKPQLFNLFATFSNQKIENKSGTGIGLMVCKNLVGLLGPSENIDLWSEQNVGTKMSFQIYAKLQDNSIKSANYISCFKQEHSSQYLSIQQHDDSLNGKDNHQVIVRSSLVRIYTKLQEKTENDINEPSSEDPDQTKIRNIMKFQSLGQKIFNQKTYQKGDSQEVQDPRQKLKQVLQNKKFGILLVDDQIFNLIAFKTLLQDMISGLDIIEAYNGQQAINKLLQNQKNLNIKYVFMDLYMPILNGWQAAEKIRTMINDREIDNVKLVALSGFDDEVEQEKCEKLGFDAFIPKPIKLEVIAEVFYQLENKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T950</accession>
    <name>A0A8S1T950_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">60S ribosomal protein L32</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0160214</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T950"/>
    <dbReference type="OrthoDB" id="309450at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00513">
        <property type="entry name" value="Ribosomal_L32_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001515">
        <property type="entry name" value="Ribosomal_eL32"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L32 AND DNA-DIRECTED RNA POLYMERASE II, SUBUNIT N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN L32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01655">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01393">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8147112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="16192" checksum="F80BE1B643F00827" modified="2022-10-12" version="1">MVVKSLAHKRIVHKRTKRFVRFESEDYPHKLRPSWRRPRGIDNRVRRRFRGNRPMPKSGYRSDKKTRYLDQSGFRKLLITNEKDLELLLTNNRTFAGELAHNLSARKRATLVRRAAELNVRLTNGKGKIRAEEKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TD79</accession>
    <name>A0A8S1TD79_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0190127</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TD79"/>
    <dbReference type="OrthoDB" id="10251809at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093:SF3">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="9"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="51"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8149474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="430" mass="48649" checksum="8FDAE78B717AFFCB" modified="2022-10-12" version="1">MYSDDRDGSQYSGNNAEYNENYQGDGQDVDMEEQQYYDDEDQDQDLQQQDDYQEYQPEEQQEDQQSFKYQPFDQRKWQWAEPLIEGVPPCARGGHSATLSGASIILFGGHYYANKDEGYKYLNDTYQMDVNANRWFKAKVQGTPPAPRYAHSAVLAGQRIIIFGGKGEKCVFRDLHALDPLTLTWYQGPEGSGSPSARFAHSATLYASTKMIIFGGWNGIDYFNDLYVLDLEVMAWSQPPCTGPSPAPRQGHTAIQVGANLIIQGGFYYQEDKNLKNLHKTANPRHGSHLRGCYLNDIRILDTEHFAWSRLRVSGTPPAPRYGHSANVSGADIVVFGGWSLNSGARSENNFVTPPDIDYLIILNTEKMCWEKAKYEGNAPRNRYGHTATSIGPHILIFGGWEYNRATNQVVVLRDLNVGQQQQQQQEKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TJ78</accession>
    <name>A0A8S1TJ78_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0240068</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TJ78"/>
    <dbReference type="OrthoDB" id="302961at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="269"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8153175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="317" mass="37391" checksum="1D910960821635AB" modified="2022-10-12" version="1">MAKDFYVLSKQSSQQCFSSLGNFESIQHVNDSNKWDDVKQSRQDLMKKYHQFKTNEAKQTTLSTYQSQKVLIPKLENFDIKLNIKRDNVMKNRDKENIISSQQKQQDKPGISSQRIRQESVYERLYNQRDAENKVGRTVQKNHILINNSNQQSKSILAHNTLIQKRSSDPQILMPISSLIKKTQKQQILQQQNQKNIQQQMFQEQNNNQELFRVNISRQQISNPDNKNEYSKQNQSIEFQPNVNVSQLPIKHCYFSLINAEEKLQNKKVRSQSTEKIKTKQSLKSQKGNRAHTQGKQEIKNQQKINRLYEYILEGNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TKY5</accession>
    <name>A0A8S1TKY5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0230025</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TKY5"/>
    <dbReference type="OrthoDB" id="71437at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="339"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="69"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8152387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="519" mass="62086" checksum="7CE04A10B2C3F669" modified="2022-10-12" version="1">MNNDVELKMIEKPEELVCKNHKKPPKYILKDQNLTQQERLVCEECMKNRDSSQPLTFDNIYDTIRKQKKERSEKTDQLLQESIKQLENVQEFIEKIQCQLESMKKATNKLIESLTNQRQQNFQFSFYEEVSIHLKEIDKSKEENDKINKIREEIINQFGKLRNQIASLPQNIDKISDKVINNIRRFYKKNYDEIHKTLEVNQINNITKQRDPCYTLTFNKTNDLLFTGCGNKIRVWKFDQRNISEITKLKGHLLTVTCIVKSNKVKNSFLSVGECGSLIIWKQKNEKEWESLKKQSQWTNSMILNKDETQLIQATANCQIDIWDVDFNQNQLTFKYSLDEYHTNTIFDISLNKKETRMVSCGADRKVIIWKKDNEDQQWTFQQEIIQITYGTKGFFLTDDIFFWIGCDQKTDDQIFFYEQKNGNFQMILGKNLQLEKDDQYIDFHLNPIIYNQQKDVFLLRFKRHIYIFGKTKDGERVIFTKLRFETNLIYATLSEDAKYIVTYIEDSKQYQIHEIKWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TMH4</accession>
    <name>A0A8S1TMH4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0220343</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8152229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TMH4"/>
    <dbReference type="OrthoDB" id="74764at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="129"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="463"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="485"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8152229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="527" mass="60880" checksum="DD9FE52C6A1E6342" modified="2022-10-12" version="1">MQQKLWRPIPNDFNILNERLYIQGEFTKENGKIRVLYGYDQYILSSKNVSQPNKCLRLEFDVKFEILRKPIPKKDKDDDSLGPIIGLQFLRDCEENMIKLRLNAATQQIIEWRSYLGERINQWQFHSMFRVYKKIGKGNFASVYQAERIEDNQQLAIKAFAKQAAYQEENGKNAIINELTIMRQLNNTHLMKLYEVYETNNSLYVTLELLEGGSLYDLIKDKVQINTKQIQQIIVGILLGLQEMHKKEIMHRDLKLENIIFKKPKKMESVVIADFGLATYVNEPVYLFCRCGTPGFVAPEVINIKDMKSKYSSICDIYSIGLVFYLLLTGISAFNGKSYSTVVKQNREANINFDIKQLKNAPGQAISLLKQMLEKDPKKRINIERCLNHPFLYDTARQMIEDENQDNSFDEIDEQIQIQSMMLQMNDNYSQFDFTKNNKNSPLESPIQSPGLVMQKQIKQQKQIDSQKAVGADSPLIKGKIESVDSAQTIGTPTKKSSVFSPSPQLKPSRFSKQTQNNPLLKYSKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TNA0</accession>
    <name>A0A8S1TNA0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">YTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0240235</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TNA0"/>
    <dbReference type="OrthoDB" id="306475at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061157">
        <property type="term" value="P:mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21134">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007275">
        <property type="entry name" value="YTH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045168">
        <property type="entry name" value="YTH_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357:SF89">
        <property type="entry name" value="YTH DOMAIN-CONTAINING FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357">
        <property type="entry name" value="YTH YT521-B HOMOLOGY DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04146">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50882">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YTH" evidence="1">
        <location>
            <begin position="42"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8153463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="190" mass="22336" checksum="02B3C74E2092CE1F" modified="2022-10-12" version="1">MQTNPICPQPEAQYLDNYENFEEITKTFKCLQKIYQPLEQGATYFLIRAPTKDNVHRAIKYGIWTSSSRNNQKLNDASRPVYLLFNVTQTSHFIGLAKIVSNFRENMHFMYWAEENKWFGSFQIEWVFVRDLPYNELSSIQQSSGKCIHELIDCTQIENGDLIYSAFSKQPQNSCMLKTFKELDNSEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TPU5</accession>
    <name>A0A8S1TPU5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0240188</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TPU5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP" evidence="1">
        <location>
            <begin position="49"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8153402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="172" mass="20407" checksum="30EDA9305D94E575" modified="2022-10-12" version="1">MKQQWEEELIIKVKYNQHLICILQHGHKNQQKKLKNVLKVVQNIQYIYKAQQAFLIYKQRVHTDKWNIDPEFILNEWMQSKQSAQQLLQSMLFQIGCGRSMNSNLGEYVEYIVCYFDYPNQLEPYRPSSSLNVANSCRLGRSSSYIALCASPYQKVQGIMQIRQRHRESQSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TQ24</accession>
    <name>A0A8S1TQ24_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein BCCIP homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0250109</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BCP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8153944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TQ24"/>
    <dbReference type="OrthoDB" id="27543at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025602">
        <property type="entry name" value="BCP1_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13261">
        <property type="entry name" value="BRCA2 AND CDKN1A INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13261:SF0">
        <property type="entry name" value="BRCA2 AND CDKN1A-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13862">
        <property type="entry name" value="BCCIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8153944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="286" mass="33505" checksum="F2685956C264B6B8" modified="2022-10-12" version="1">MNQGNQDKRKQRDYDDEDSEEEEIEQPISVHNKGDMEEEINLDFVFLDPNQKQFHSIKTFINGYLEGISFKSSELANIICDQVELGTMVGQEDEDNVFGFTTILNIGEIKSNAIGEILHYVDTKSKQFNKQHQQLQHIFQTPKKVGLFINERILNLAPQLVPILHNQLKEDINWLQKEDPSNSLTNLDYLLVITKCFKDNDQQKQTQKKSTSDLNDLIFQKFEDYVFLQKSVVSFRFLSEGSKQTQQVSDFMKTEQEGQICYRLIYLIQLKDYLEQIVNIEKYVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TUQ8</accession>
    <name>A0A8S1TUQ8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0280080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8156000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TUQ8"/>
    <dbReference type="OrthoDB" id="301661at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="160"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="243"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8156000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="356" mass="42125" checksum="61F6C517DCF3530C" modified="2022-10-12" version="1">MSEQAYQTSLKMDLLNIEDEISKLNKLGIQQSRLSQNHTQKIKQWISNIKMNDKCLDPQRVIQICNCSDANETVVKLYNMIQNFDFYVNKITELQCIHYLQYEQCFTNFQSNYFQKIASQGILPNRFLMSLLNLEIIEYLVQKKGDMNQSNGESILQKKLYDLKDENIKLLSDIKSLQNQCKKLQDQISNSTNLTQSQLSNYSIELPPNPTSYYNKIIDEVKDSYQNLVTMLRDENLQLGLKLSEVQQKFTNTSNEIIELNKKVDNMKSQKDDLYKRFVAKYKTTEEFEQSYEAALKEEYSSMEISFKQKIQQLQQNIDQNNRDYQKKLLEQKQIIDQQSDKERMLIKKMSLYQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TX07</accession>
    <name>A0A8S1TX07_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L37a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0300110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000030">
        <property type="protein sequence ID" value="CAD8157355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TX07"/>
    <dbReference type="OrthoDB" id="308782at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002674">
        <property type="entry name" value="Ribosomal_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050522">
        <property type="entry name" value="Ribosomal_protein_eL43"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L37A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01780">
        <property type="entry name" value="Ribosomal_L37ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8157355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="15442" checksum="4F94E642AE1DB22D" modified="2022-10-12" version="1">MTKKTKKVGITGKYGTRYGASLRKIIKKFEISQHQRYFNTFTGAHSLRRQAIGIWRCTQTGLQIAGGAWEVNTPAGLSAKQGMLRIKKLKDDAEVEVKEEKKEKKQQPKEEKSKEQPQTKETKKPQTKKPQAKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TXE7</accession>
    <name>A0A8S1TXE7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0290157</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TXE7"/>
    <dbReference type="OrthoDB" id="294828at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035902806" evidence="2">
        <location>
            <begin position="18"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8156764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="112" mass="12819" checksum="A95CFD0F7CFB637A" modified="2022-10-12" version="1">MLKKIVLLLILIVAIQSRHHRLRPTKSDVGHNLAMKPGDACLPTTNLSGQKKEETAANQARKHLTKFADDLTEENRKATEKQPFPNLYTYQDDNCVGKAQDPPKRDRRMRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U110</accession>
    <name>A0A8S1U110_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0310290</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U110"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001133">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="58"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8158340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="447" mass="52276" checksum="0F026D432793BFC1" modified="2022-10-12" version="1">MNRKNQMTIRASLKNTLFNKNRRNLKQNLPPQNSSPPIQNYQTKSTNQQFRHFSDIYEYGHHQIGEGAHGIVKKCYKRDQNNMNISERTTYAVKIFRTGDTEIINTIRETFHINRTLNDLNCVVKALDLFINSKKEEHHLVMEYCPYMSLEQRMGKLCLEDIQIIALNLAQSIKQLHSRGVCHRDLKPDNILIGDKLTLKLIDFGVSKRFLLKGKVTKLIDMWTRTGSLFYQAPEIFLGGGYNEKVDIWSIGIILYQLLVGQLPFQQETVLDTIEMIRDSEINVKNMLPFKKLNPLEQDLLKRLLKKDPEKRLSAEDFALHPWLHKGQQKKSTKSFDDCDILDIRKSDGMLQQSEISIQKQYLPRSKNPLIVPIQEEKVSSPLKLSWNYWDTHVHYVPQDLIQMINLYDRQSKDVGNSEEVMNQQQKQDEDFQVGLMRTTSEHFDQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U2V3</accession>
    <name>A0A8S1U2V3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0310291</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U2V3"/>
    <dbReference type="OrthoDB" id="312214at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8158342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="472" mass="56176" checksum="D95CD61939A79724" modified="2022-10-12" version="1">MQKLVDFSELQTLQIDQIYSLFISLIENSKCKPDSMSQGFICPHTVSCNLEIMQFDDKKCNICSNQIFLSKKTQLSGILFFLLGGLKEGQGKVEEFYQNIQDYFSPIIGILSEQQIFRSLCILINETEFEKKLSMYEKITSLNEQIQPKIYKYKLKKFCIVTVDPNLGFLYQQNELEEITLVTSFKIFMEQYQQLLINLKLSFKDFKICIHNLFLGSKNKKYHYLKFNLFYDYLCKQCHQNKFSLLFYEILINQSFQRNSQNNIQSLTNDVHQLSKNQNEENDFIELFHLLQKYLNNQGQMAYSGFFTQNISFFSRDIMQQLKTFQTQDLLWQGYKILVQQEIVELIENSINKNDTICNSAKKKLFDSYQVQVQLIQAFNNYQSIPIFDKIVKKYSDLIQDNNFQQIDDINNLNQSPLLQKRQQDYQFMKQFNTFTDYKNACNQKQIQPIFQEIDYRIIKQYEEQLKDGIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U7N5</accession>
    <name>A0A8S1U7N5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0350147</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U7N5"/>
    <dbReference type="OrthoDB" id="10426882at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8160738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="149" mass="17487" checksum="0F867CA19EA7E2DC" modified="2022-10-12" version="1">MKNSKKTNKFQIHRQQHQIHLQNVIYPETNNKPLCKLFENNNNQQTISKEVINKSKSIMACKKSLQLLSLILQQQSQRQLKKVNLNQPAVYKSLIIRRSLPAVKPSNQPIISCREKKSYSVQQSRRYSNSQNKINISMRETKLGPWEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U8C4</accession>
    <name>A0A8S1U8C4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0360001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U8C4"/>
    <dbReference type="OrthoDB" id="299342at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="80"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8161100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="326" mass="39144" checksum="7D7B3948D8B32280" modified="2022-10-12" version="1">MSSDSQRNYDLYEKIGPLLSNLSSINNNFEQQKKQLNSLNYSIDKMNIDIKNKEMDQKNLQWLYSLQERENRLQMEISQNEAKKAQLEYLKKQNMFYEEQIKMLNNENQKIKNEYLQIQQKNEQLISQQEIALKESIKQVNEQFINRIVQLEAQLQQKQKDEVEASVKIAQFVNQIKLLESQKQIFQQECEQFEIAKNTLDKEKEMYFNEFRYLKENYMKMIDKLQEEMHKLKVQNHSLNEDNKSLKQTVEYYKNRFQDMMDLQEMASLNQDNSLLSLLKSKGMQNVTKQDLVQARDQVAQDMVYQKGFQFGNTWDQVMNDINKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UC91</accession>
    <name>A0A8S1UC91_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EIF-2B GDP-GTP exchange factor subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0360102</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UC91"/>
    <dbReference type="OrthoDB" id="10249309at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051501">
        <property type="entry name" value="eIF2B_alpha/beta/delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45860">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45860:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8161302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="323" mass="36671" checksum="748DA441B99D9889" modified="2022-10-12" version="1">MNLSFQSKSDSMSITLKKVLDEFTQLNETTKEHYAVILIKVMINLIKNSKEMTMQGLTHEIKTAGEYLINNAQAGHGRSELVLRSTQTIYQHYISKGLQHSKADSMEALQSSLIEISQELIDIIIRSKDNIKTQCLKFFNNRFKVLVVGYSNVVIYSLIEAFKAGIVMQIYIPECRPKSEGIETYRQLTEIGYPCKLIFDSAIGQVMENIDMVLTGAEAVVENGGIINRVGTYTTAICAKFLEKTILCFSRKFQIYTFIPFIIKRLNQEEPQFSEKLKLPENLDFINPLCDYTPPDLITLLFTDLGVFTPSAVSEELILIFNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-06-18" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UH14</accession>
    <name>A0A8S1UH14_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0410040</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="295668at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026847">
        <property type="entry name" value="VPS13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026854">
        <property type="entry name" value="VPS13_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16166:SF93">
        <property type="entry name" value="INTERMEMBRANE LIPID TRANSFER PROTEIN VPS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16166">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12624">
        <property type="entry name" value="VPS13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="920"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="629"/>
            <end position="660"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8164451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="3648" mass="427356" checksum="E8F3B1898289505C" modified="2022-10-12" version="1">MFEGLIQKVLLAIFGRFIDGLDKKQINLSFFKGNLVIENVSIKKEALEALQLPIELVYSSIQRIEINLPWNKLTTQRTEIKIHSVFLLVTTISEEMWGLEEINYYKPKAAQVRAYIKKVMDEENRKQETTTDNQESDQKEAGFFQKQITRIVDNLYISISDVHFRYEHCISKHSFSWGITINHITQQTVDSEWKISQASSPYIHERKDSTFVNKQIQLSKLAIYWNPDDHYLILSSIIKPQQYKNVDIQKVVSKMDPYKIDLDLHQRTVYQIDEKMQARIQSRLQQELVYEPDNLTMGIKTSKSSKYIFCLNAVANVVQTYQHFRKVPEYKIKVELQEMDFKIKHTQYKEILKLVSIFNLYQYNAKQQMDQYLTSVMRPYQQDFFKMETNSLYLRSLFQYLVKTQILEIRRKRFLEFEMNALQRKTTPSALLKSQHRELRQYQQIVIKTSITTLQMWAKQALEIFERAKEHHQIEQAFKKEIEKAYKPSAFMDEETYVYLEFRLSIVKGSLKLLRKHKNLEEGFTLLWKNFDFFLQKRRNNFELEQIIQDMEVQMFSVEEKNDIIVPIVKKIKSYDIQEKPLIYFKLEKHPLSKPNYFSSIYLDVQKIEFMVFVASLQRVIAYFRTPPQDDRQQDSKVKEDANKNKKEQKDKRDDSDQDKKIEIQTKNYINITIQSPIIIIPNQQINDLHSDCWVLKLGDFEIITVDDRQVPGFIKLGSDQYRKLGLSGESMGRQKIVEQPLLTVHNEKNQLVSQFNFCNVNSINPLEKSASLQNLLQPQLQQGIKQKKHHKSQNYDNYVMKLKSVQLQYFSSIHYFYNYQKLSQTQRGLDILNNPLRMRSKKDDVLEDFSMELNLYFKKSTQVNIPKTKINFELPSLKASINPEMPYIFQKLMQTINDLSADQSLSSILQQEKSKLIKYSSCKGILRKRGKFLRSWSSYFAVLSGWYIYLYQNESDVDYNQYVGLRDVVVSESQQDIGVQNSFKIKSKKEIIYFQAKTAQEKNRWFKFIQQKIHDIKTKTGNLRIGSENTVPLQNDKSKREFEQTVKQVPKQNDELLTKYRFQIKRFQIELKYNLKSEILITFDQIKLLSLSGITDNYQEFTIDSISIEDHGQSILRSIHYSQPNTPNILKVKQNMTENKLLRKIKQVVNFKEEDTYHIPGAIRVRMCNTDTHQPNEPQSYIVAQLSSLEAHYLESFLGKINLFMVQPSLQKTKDSTTDLSEYADNTLQTTNMKYTQIPVSETKFMQTKYDQTKVNIKSVRSFTLQKSSVSDSDLHDGQQAEGIDKVPESIIKVSILTQDIKLIVIVQEKEKDKEKSNEFLQIQVNQISAVLTINDDEYFIEGNIQKIEIIDLKSLQGNRHMFLGKKEQKDKKKTKEEKNKKNIGLTIFSNKHQVEKQMVQFKYSKFKIPSNNDDISDDLYLIVRDCFIDYLHATIIKVLSLLEISQKKKQANKEENELDLELIKQKLLAPSLIKIKILLENPKILIKPAGNISQSFELQIKQVCFELDANQEKNKVIEMAIKANKKQNQAGQQIDSLWWQIIVVSADTMRILIRSTSDLQYISNPFSGVLRIQTPMFLSEYEILCPNIVLNRNQEIRIKVTDIQLNILRNHYLFLLQVMQTQYEIQSQKTQRKRKQQQQQQQQNKKQNQAALIFKLSKITIRLMDECNQYASQGSFLRPLIDLSLLDIVFQFKTNLSLSVQDLKSTYYQYPEQDDNNLIVRIQHVINNIFLERRKLEEFLDEDILEDKVKEVKELINEMKMEFNNEQPVSRFDVDDKLSIKAILGEESTDVSINIGESKYLINAYYLKTISLFFEIHKYEEDVNSQQSPISQQPVQNYYKQKQALLNVNVILLKTLIITHNEKLKNCLILQLDSFMKLNDELNNQAHSNYHQKNKEKYSNEQNIKNRRFELSLTVSLFNANFAALQKISKQTKRKMILYPIKIEFIRNQFICDCDINYSNYKTDGQNELISNIIIQDQLRFDKIRINISHNDYALILQTIKFQQDSIKQCDQFFIDDQESSVNETIVRQMPIKPTLGVEETILSRNETKKKSFLPGKSSLIRLKSSILQGSLLKDEVVQVFYAAGDQEMEMVKEEEILSEQEQQFETIRPSIKINKMKIKLDLLEIVLVNDINKSFAPLLLFRFNVKKFMFDQNQISKALKCLFQIELMYFNPVVSKMEPIIEPYLSELLIVQQNQFMQTIHINGLTPILDMNLSTDMIINLYKTLTQWQQLDQDDDEEAFRRNESKKITRLASMKGISFKENQDNVTLFSLLNKSGYTLQVQKNGNDGLIEIANNDSKNYEVQSHDEDYAKTFESDTRLMIKFIDSNMIQNSISNVPINYAGVKKLQWNEQTQYYVLSTQIEKLQKTITIYSPYMFVNQTDCDLSIKLSCIKITKNFILKPGEQAAMPQELYKDDSFIVIHFQDSKYTKLTQPNEPIFIKRIFHDITIQILHYPCYTLATLNTTLVQNVKYIEFKPIFILYNLLPVPLQFLFHSNQNLDLQKLMINHYKKPNIGIQYEKQQSGYSRGNSLSHLQNEQHENLVRSVTVTEFNYTTTIKVEAGLIEVVPQGELQMHWLKNGDHLGINLHVSGFEISQKQDIIQATEFELKDTINSQSCHIKVEKEQVKQTKLIYFSVQTCIINTTNRRLKYYTLKDQKPIILSGQNKEPKPDKECFESNYEKIQITDTIKYLMIGIDDCISNEVNVGLLGSIGLQLKEKNGNMVSLNEFGIHISLANNVIRKGVYTKVIEIMPRFVVINNTGMQINICQSGFESQPKKLNPNKRYQLIWPDCRLEKSIRIQKYGKYNWSGDLFINEIRSTSFFSSSFNENSQQLSNFSIDQQKSRIFEKSGIYKQFNLKKYDPQEFLQYKESLPLQTSMMCSSSQEFFKDCLFLTAEITKIKATNFILIEQQNEENQLYKLVNKCRPFKIHYGQDSYNSFQEWILDCDQTVPFSWEQPSHSFKLRVKFVYNQKIEHIEIIDFQQQESLQEIQFSNYFIWIKSEFQGQTRVITFQQVNESMRNMTTSHKREVEDIHKLIKLYISQLGISIITKRQNILLEANYIFMSGIELGIMQTENQTTAQLRVKFFHIDNNTSLDTTTPVLMTLRRYYQVTHDRNYYFLEASSSFEHQVKAILLYNSIKIYIQPFQLQFDEEYIQVVLETIKEIKSNLQNQFEFEYNGSPHSIKSLCFQTPQQRHYYLESMFQQNSSNPCIVWKSLEINRSKKQVYIKEFVLSKIDLRFSFHKRFRTTIQYDENVFNLFSTAIGATVQNFNEAEIILDCFRLRSVYDSKEIVLQSIQDHYKSEIIRSILKIIGSIEIIGNPVKFVQQITNGVVDFVEMPIQGLRHGPLEFGVGLCKGTGSLLKNTVAGAFYSVNKVTGSISASVSLLSMDDDYLEKRRIFMLKRPDHVLDGLSQAGHCLYNGFSNGITGVVTQPYQETQKDGIKGFVKGTLKGLSGLVVKPIAGVLDASAKAAEGVVSTATHFDDKPNDTRIRFPRIFYEKSKYFQKYIALDAQVVNFLIGFMNGKYKDIELITCFYLKETETQLNNMQRQILSATKSIHECFKYTSKILILSYQYILVLENMTLIQEIITKEILEIQEPINGFLRLLIENKQNIEINILLDEIELKKATTLLDYLHQQVKIQYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UL53</accession>
    <name>A0A8S1UL53_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0410243</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UL53"/>
    <dbReference type="OrthoDB" id="636685at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00084">
        <property type="entry name" value="HMG-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050568">
        <property type="entry name" value="Transcr_DNA_Rep_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252:SF54">
        <property type="entry name" value="CHROMATIN ACCESSIBILITY COMPLEX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252">
        <property type="entry name" value="HISTONE-LIKE TRANSCRIPTION FACTOR CCAAT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09011">
        <property type="entry name" value="HMG_box_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="5">
        <location>
            <begin position="34"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="4">
        <location>
            <begin position="92"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="174"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="177"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF09011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8164847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="202" mass="23716" checksum="F97C3F228B028B56" modified="2022-10-12" version="1">MKKQILESGQKVITIDDTINSKQPTPVQPVVQQLSAYQLFMKDKKDEGKYSLQVLSQMWKDLPKDYKEQYQERARQLKEQVQQNQLTTPQTFFPLSRLKTILKEDEQKPQFKPEAYQYMLKAIEEFGELLLNKVKDQIKQDQRKTIKEDDLINLIKENQELQFLSCLAIHHQKKKTSQTQNQQGKSKTSGALDKFLENNNGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UNE4</accession>
    <name>A0A8S1UNE4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0430200</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8165934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UNE4"/>
    <dbReference type="OrthoDB" id="287389at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8165934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="220" mass="25827" checksum="AC2614B3BE2CF889" modified="2022-10-12" version="1">MKNESESRVSIDNLSINKATAPLVTNYKQLVCCFVKQMLFDVVIPGQKMFTEDKKQNLRNQILFYQQGFARNMNDEQAYSELIMLSTSSPQFIELLKMTCYSLIGQNQEIEAQVNKELNRSISSIETFCGNIKMIEKNIIVNWSTNRQKLYSKTIIDIIMRGFLLSYILEIIIRSSLKQDFTFNFAITIEYNQCCLEKEVQPIFKINDQTTQYEFKFYKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1URV9</accession>
    <name>A0A8S1URV9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0460149</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8166943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1URV9"/>
    <dbReference type="OrthoDB" id="291656at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="787"/>
            <end position="910"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8166943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="912" mass="104591" checksum="139D892404647932" modified="2022-10-12" version="1">MKVFINKKMVLAFQYNITLVIKIIDIIIMALCARILYYLKEQKKSGYPLTILCLVRISAQLTLNYFCKPQILSVIINLNLIHTLFQEQLVSDFSLAKLPSLYIIGNVFFEQQFSNYFINVGIGLTCLSIMFSFRHNNKLKQKSEKSIQKISKDFDICISALPQKEFDAYKSEDLNASNCKSPVYGGFSPLKELSKSMDSVLMDEYAWIKGQQFIVLDDSDNVIIQTFNVADFSRNSFSNSIDNESVASILNLAIVQGNRDFQDLIASSGTINQSRFTLKELVNKLVGDYELYKNNMFIINKVDASWMNDWMIKIFLVGKNKKTFVFIQIEQREVVYQPSFQAFSQFTTYITPSLNSIMTISVLAESDPQISDYILEKYMYPIRVSSVIVYLQLANMRDFNLHNQKKLLLKISTVDIQSICEDLIIMVQDSSKAKGIEIKLEHESTDNIIETDAERLKQLMLPLIKFCIKQTKDDSITISWKMFSTKNYQLIIHTPINIDEKDLRIIRLNLKKPTLQFSEFSVCHILAQYLSGSIKKGLEIAQVEPYGTAFKFTLQSFNINNEEMKAIRLIGQTHYQETSLSQLLAQQDTSKQNEKTYTLFREESNKASMDHSLHFSQSRISKQFSQKYSEYQSNYVSQISKVKADSVNIPNSKQKDSHSFEQISNIFYQDEETKHQKRINYQTTPSQSKYQLPHFPEATINNSLGNSANGNNSGGAVNTLLNDAREREQTFLSQPQLTELKQQPTSSNLFLDFGTDTLSPIQIPVSVSAKMQGEIIKFSTTGRCCSRVLIADCEYQNIQILEMILSRFKVNSSRAFSTEDVIKLLETKKKCKCGNIGFVLYFINVDLPKKGGLWLSRYIKDKMTQKGYIIGTTGLVEYYSKIEYYRNGIDQYISLPFDLAEVSKILQISQHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UTL1</accession>
    <name>A0A8S1UTL1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0480153</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000048">
        <property type="protein sequence ID" value="CAD8168031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UTL1"/>
    <dbReference type="OrthoDB" id="407812at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000154">
        <property type="entry name" value="Alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051044">
        <property type="entry name" value="MAG_DAG_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11614">
        <property type="entry name" value="PHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="68"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8168031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="320" mass="37015" checksum="9AB1FF56BEFAFDE7" modified="2022-10-12" version="1">MQFFQKSEEEIRIEQKLQRINAAKQEEKYRKPNPCQVTFPEENFIEMKVQQEGKLIKLNTYRFQANGKPKAVIFMFHGLCAHINHCAHIAQKMAQDGFLVVGFDNRGFGKSEGIRGYLESLEIHLSDCRLFMQKVLELQGNSNIPVFLSGLSMGGMTSFRLAIQGNIPNLRGIILYAPAIKTLFSNLQINTIKFVGYIIPKYKLIKPKRGQTTKNPQVTEDLMKDPYTYSDELLPKTISTITVSMKECESMYRQLQTPWIVIQGGLDKLVDPDLAFTLEKESPSQDKTVLYYENLWHDVWHEEEIHDIIPKVIQWLNQRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UUP8</accession>
    <name>A0A8S1UUP8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kelch motif family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0490068</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UUP8"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF3">
        <property type="entry name" value="KELCH-LIKE PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="243"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="65"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8168401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="598" mass="69146" checksum="4350AC9DDCF3FE50" modified="2022-10-12" version="1">MDTEEECFSGDEIDQFNCPKHLLIYTNYCTTTQQLLCQECLIKCSLKVQNIISIEKALNNISLQNKQQLQKVQKIIKNYNNLDDQIQKENTQINKMSQIQIQSLNSICSQIIEIVQMKFQELIEQVEVHKMKYQQTLQDSKCIIDEYKGRGIQLEKNIVNQQNTSGYEQLKKFREFYTINCKTIKELENEYDNLSQAPKIQYQQLQIRNTTDIVKQISNILSILERDSDYRIKRVSSKLFQQPNTTRASPFSKKGFTQHSSPHFSLQSSKQQMRKTEIKQITLDIPTDLSSILDKASPEQSIINDFFSNKQSLSDHSISQMEIPIQNQTQFYIIGGLLKGQRQNLIELVDIQSKQSSQHDFLVSNRYGFQIAYHGNQILLIGGKQEGIRTALCESYDPNLKQSKISQLILTKGVSGLASKQIGNKLYISGGKTDQGIQDIFQLINLDTYQTKNLQSIPIKCYNHTLVQGNEPNSMYLFDEQGCFKYDMAINQWKKIKSPSYPRRSHISLTLPDGIWIFGGRSGKKYIKQIEKYDETDQQFYQMGELICPTIKFDAIVSDDYQFVYLVGGKTQENIPSSNIWSQFTKPRYSHKLIPKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UXY2</accession>
    <name>A0A8S1UXY2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0510102</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000051">
        <property type="protein sequence ID" value="CAD8169525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UXY2"/>
    <dbReference type="OrthoDB" id="312779at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8169525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="386" mass="43719" checksum="C305E7B0DEEF6626" modified="2022-10-12" version="1">MNYFACPIHNGHFLSLVCTEKSCNEDPLICPLCEHADHQSHNTLPIKLMLQQVDDAVNNNCDATQQSIMDVSIFLDKSYNECVRTVNRFNEQMQNISKQLAKKVETYYLDLKNQLRNFSLSKTPIHFLLDSLNIPVELIEYKKNIEQILEQYSFGNGIAVRQSINSAIMIACKSQITQLNDSLHDIQIEFDNKVETLSNIFKAQPVTATDLVQSIAFQFSQKFKGANIGLSGQNMIAEQKTSESNGQRFIICEPCLPKNGVYKFGFKIIKYAGWIGVGVCHRDIIASANYKFNYTNIGHGSYLISNNAYSWSHLQKDLNSAHKSFDFGVGDIVVIEVDIPKKKITWWKKKSQLKFSMNLDTNLDLYPCANLCSPGDTVEIVNKVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UYC0</accession>
    <name>A0A8S1UYC0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0500043</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8168892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UYC0"/>
    <dbReference type="OrthoDB" id="308568at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051500">
        <property type="entry name" value="cTAGE_MIA/OTOR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23158">
        <property type="entry name" value="MELANOMA INHIBITORY ACTIVITY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23158:SF33">
        <property type="entry name" value="TRANSPORT AND GOLGI ORGANIZATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="96"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="157"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="344"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="104"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="128"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="163"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="199"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="233"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8168892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1267" mass="151883" checksum="412499B23F7E38A0" modified="2022-10-12" version="1">MYRRNNFNRGKPYRKPQQQYQKQNYFQKQERAPIIQDKSILNDPILFDNPPTKKIINIINKKEPDYKYKNEDFQKNNKEIQINDKNKLLKLNHFKKNYDDRSPQSKSLSPENSYENNYIDDNDDDQNNQKINNYQNNKYSDLKEENNDIEKVSQKLKNLLNPVDDEKEEENGDYDDDTDEQDQNNSQEENDQYDEDGEIEKNQKQDSEQDDQQRDDEYSDENDQSEGGRDNMDDNMDDDMDDDMDDDMDDDMDDDNQNDFDDYDDYDDQIEDRVKSPVKQPRLAESSKRMLAFQMRRIIPSLPREFGQNIQKDEKAKKIPDQFNYQSQNQTNELRQTQNKDQINEKLIAKRKELQLKYTEQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQSYFKNLQNEIDANSWKIPQFSQNLQPISHNTGANTIKYNCEVKWKNFQFIDSMCTQQNIQDAINLNMFDSTFEVDKNYQLNTSLIVKQYIRPNQGKLETIFRSDVGMMLSIQRLHQIFCDRQKRKKNGQLVNIVEIFQYISDRFRALSTELKQIESENRSSISNSQVKQFSCQIVRIYLLSYLEMMQADQSCPLIQQNKFNCNKYLIDALDDLAIDYKILFNEINDSRFYLLDNLPEFISYYHIMLSQKILSTNSIQKLANLDNIIFSEFKYFEKLIKTSEYQFCQLMKEYLIQKNYSALLDLGQNSNITLFQQFFIYLFQDQIEQCLEKFKQQKIFQNEKPNINLINIGSIYQKWIQIEDKIQFINSKGNFSKIIFEQGPIENINYYLRDQKSNQQEFQQEMLKQKSQQIDQQTKKMKSSIQEILLQFCQKRVLNKFTKFLAPHLDKVDQQLGNFYKMQRILKHWRLSTLTEKRYKQFVELCRQKNKENIKKQIKIIEQSNFRIDLSRKLIKQNADKKNLYYKIVILNNDENHQQLVSKLIFTLCNQQHTSQDDSIIIESNITSQKNIYKLYMSIQILQNLEKERICGADIFIVIGDILKVNVAKPCLQIVFDQKYQNENLDTIQVDYEYIALFDKLYESFEFYNKLNEIVDKGLKNKQATEDLKLISLGDYFKEIQRTECEDYKKDQIEYFITRCVQAELCLEDALNNNIYSDDFPQELLFDRYVDQELILKYVSKFNPANRLMIPLCNQIQKNMINERVLLNTIKDYLDEYLDEEALLALLTKNFKFGNNIQGDWKLLFEFIMEELVQYLENSDIYFVLKRSYNTILDQNKLKNRVQQDVQQLLKKDSNQQQNSQNISCDLIKKKVCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UZL2</accession>
    <name>A0A8S1UZL2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0520169</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000052">
        <property type="protein sequence ID" value="CAD8170131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UZL2"/>
    <dbReference type="OrthoDB" id="194358at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="280"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8170131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="340" mass="39561" checksum="F15F15C1869E7E9D" modified="2022-10-12" version="1">MHSLPRLTSQSINCHISNKSDSLNGRRLHLGENVLYRIHKQALSEYLKSGVIDQKIVQLNLLKMSKLNSQDLSILKDVYTQPYSSRFEQQQAKNILSKIKTQRNPESINSNEKNQIELSQEEIDFMSVEEQLKQCLRSKINTSKVFNGTYLIDPTINDLKKINLQGKRQLHNLLNIEPKKKVQKKTSLLQRQSSILNPQHCRKPSIQFMEVEDVTQYMSSKILESKANIDQLVERFKKPEISEKQQYFFRLIQNCDCKALNSLFQEKSFDTSIINTPDEKGFYAIHLAIKKQNPLLLILLLKNGARTDLLTKNGKSVQQLSQLYQNQEIIEILERQKYNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V3B3</accession>
    <name>A0A8S1V3B3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">H-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0560003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000056">
        <property type="protein sequence ID" value="CAD8171938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V3B3"/>
    <dbReference type="OrthoDB" id="307628at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019019">
        <property type="entry name" value="H-type_lectin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09458">
        <property type="entry name" value="H_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="H-type lectin" evidence="1">
        <location>
            <begin position="40"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8171938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="425" mass="49474" checksum="CD81BDCE1495F630" modified="2022-10-12" version="1">MIFITLFLFAQGFIRYDENTFRSFQITNKGFYCFNNIGRTQDIKFRDSFENIPKVILIPELFDIPGGVANYNLEITQITLTKFTLSIKCFDGVIYGIHYKWLAIDDQRIQVINQFNQKNFESLTLDHSNPNAQNYLISVIAFSFSGSVSFQVKVSEINTKSLTIIVTDLQNTLPNLISISYQIILGIDETFQQQPLALINSPYTSQEFAFIQSSWFLLPFYGFQYDGVQEIRFKKQYFSNVNNLWYDMNVGNFQSYATGCCCMPTLWHKQSWMKFEYITEFKPYEIGFISIKYFKDSKVDYLQSFKIEIQGDLQKATNLGLTRIIIDKAEQQTNLKIHTKCQEKETMKIILFIPNSTQNSITNPFFHSCLDSFEEVVITFTLISTSVAYQDLIVDISETECLISQLLFNYRFSQVDLFSIQKLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V4R0</accession>
    <name>A0A8S1V4R0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0530269</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V4R0"/>
    <dbReference type="OrthoDB" id="296936at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8170832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="239" mass="28560" checksum="E57334B0578F419E" modified="2022-10-12" version="1">MNQTTPKSSGINYAKLRKQKSKDTTIPKSMSNNLKFDIQQNHQYGILKHRSTSQHNIHEHVQSQQQIQLFEQYFNQSKHLLPFIEFVTKQIQYHTQPIQINANIFKNGQRRWDDEKRRILNELNQCKEDNCLLQSEIHELKRQKNTLSKQCENLQVIIVQERLGKQQKHHHHFYKFQELTKNIHVPTQETISSLTDERFESNETSLDSSLSNDDHHHPNQLFELLKTGKHRYQFSKKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V553</accession>
    <name>A0A8S1V553_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0550170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V553"/>
    <dbReference type="OrthoDB" id="426293at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="39"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8171727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="278" mass="32134" checksum="7DBA7E00DC432781" modified="2022-10-12" version="1">MNNKKDQNLTAQGTQIKGAAKQPTHQVQQQKPAQSHPVQHQPANKQQQQQKPTTQHPTNTKTQTKQQPQQQQQAAKPQQQQQQQQQVQQVAPKKLEPKRTIKLQAMLGIMDQDKEEGKISFLIDKTGNAYTFFKYKGTIIDISSFVITEQNQGKNHEENVEKARKCVVYGLEAGDNLVFSTDITATNLNEFFKSDWYKPEAIFNYEEITNPKYFRAHLLKPEEDKDGFGNKGQFWPKKELHIVLLYTQKEGEFDFEQHLNKLGLDKLKNQFIVTIIED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V7W2</accession>
    <name>A0A8S1V7W2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0580031</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDO010000058">
        <property type="protein sequence ID" value="CAD8172761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V7W2"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000099">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF803">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="10">
        <location>
            <begin position="92"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8172761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="456" mass="51701" checksum="4EDBF6258BD5EBC3" modified="2022-10-12" version="1">MNNKGTFKNRDINTFLTNYPILDKQTKKPFEYLNLAYSKQFDYSPIKKQSYSSKANETQYKSLQETSFLRQNTLPNYEPTRCSSLKNGIIKAYAANTNQGLVRDYNEDRVSIILNIIKPQSRENEQWPKCSYFGVYDGHGGSACSDFLRDNLHQFVVKEPEFPWNPINAITKGFEAAEKCFLQMAQDSFNKGIPERSGSCAIVVLIIGDTCYVANVGDSRAILSAENGKKVIDLSKDHKPELEKERIIKGGGQVYQTHGINEDGQPILGPVRVNPGRLSVSRTFGDIEAKLEKFGGNPKIVIAEPEIKQFKIINEHDFIVLGSDGIFDTMSSKDVINGIWKDVQSNQNTKDLPYMMSNAVESVIKESLLRKTTDNVTLLIIAFSITPLREEEIRVKTISYIDKLVKIPQSSNTSNLRTSKFNDENNPFFINAQKLKQNPKLTKFNFEDNLRYRLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V996</accession>
    <name>A0A8S1V996_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0600230</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000060">
        <property type="protein sequence ID" value="CAD8174108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V996"/>
    <dbReference type="OrthoDB" id="421226at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="220"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="263"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="287"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="335"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="409"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="3">
        <location>
            <begin position="512"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="754"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="849"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1055"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="754"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="849"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="863"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="874"/>
            <end position="895"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8174108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1074" mass="125941" checksum="BD684EBB51CBFD66" modified="2022-10-12" version="1">MNISNEHVQNSFSLQDEFFQGPNSGMISGRIVLGEGESNRNLPGSWNSFTVPEQQKLGDISQQSIHMMPIDMHKSMKNMRKSEYQSAYVIEDEEEQGGKPQFLKLIIAKSFQNNFINNLWNRSYLRKLHQLSLYQIQALDDLQLENEAYLHNDKRTLTRWEAFKRFFSYIEVFTPYSQLIFIWGLFQILTYLLIFFWLPYKISFKLESIGDFLGISIYKQSLEIVFLSILSLDVFVGLNLAFIYKGIIIRNRKRILINYFRQYAFVDLVSLSTVTLQFFILTNDNESNQMLIIQIVLCAIFYVLRMTKINKILAQIQEFFNLYGSLNDLVGLLKLTMMIVFIAHICACVWHGLAYYNDGYSWLDAYDLRDKGNASKYNTAFYWATMTMTTVGYGDITAKNNLELLMNNLTMLIASIVFAYSVNSIGIFVSNMYKGAMEYSRSVTLINTFMSKNKIQFELQTRIRSYLEYIWQEEQNMNDEEVSSLICKLSSNLQEELQYQLRGNILSSCKVLIKTFSEKMIKQLLGQMEEQSFSPEERIITLNQLDDSSLYIITKGEIEIIFEGFNSLNERVQRNSLKFLYQGDYFGEFSFFTGQPRKATAISRAFTKVFKIKRENFIKILLSYPNDYEKYCQLTHTLLHQDYSSFQVSCYSCSSNQHLIDKCHYLHYCPDKESILKKELYPHDQPRNNKIGRVNREKEVSPWLIQKYLMSKAREIQQEQQYLANKGTDFEDYEQHSNLMNDVYEDEIEIDVPSSLNQRSNSRSFSKISQKPNQQNMITENEDDDSKNYSRKPVNPRVTLQTAGFGGGMRDSVRVDLMKDDEYQEISEEESEEEKDQVLMQLPMNTKQNQEITRTQQQKITFTRKDSSEEIIPRQSSLRSHTYTQKGRQSQTIKRTLTPEKIFPPNSSDLEVRNHQKRATQKRQSNTTRTFTRNQGREMTTDNNHTSIMDNQTYNQHTNNSIFNDFDKMQIFVYYFPLNNYDIVIKRYARLQKFFGKRRLYPEFSKYSFFFLTIKKGWKLRKLGDKITGLRIAESLKKPFTKTFQSIKTVKKIAHNTTGNGGGGTSPLRLQSFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VBT9</accession>
    <name>A0A8S1VBT9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">cathepsin L</fullName>
            <ecNumber evidence="10">3.4.22.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPENT_87.1.T0570036</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="11">May be involved in extracellular digestion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Specificity close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity.</text>
            <dbReference type="EC" id="3.4.22.15"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.15" evidence="10"/>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VBT9"/>
    <dbReference type="OrthoDB" id="387093at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000104">
        <property type="entry name" value="Cathepsin L 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035863173" description="cathepsin L" evidence="12">
        <location>
            <begin position="25"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="14">
        <location>
            <begin position="29"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="13">
        <location>
            <begin position="110"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8172326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="308" mass="35134" checksum="380F180D034DFCB9" modified="2022-10-12" version="1">MKYLIGSLLISFLFISNYFNSTQFQSSDFENWQKQYGYFNTQYERQYREMIYNQNKKLIQEHNSKQNITYRMQENQFMTLTHEEFVGIYLSEVKESIQEMTILNQDLYNPLQAVDWRNYTTVYNQGSCAAGWAFSVGSSIESWFYIRGGGQNISVSVQQLVDCEKNSSGCNGGINTQAMLYALKDGLYNSQNYPYVGTQLACKATKSGTYFINNYTFVGGSSQSLQSSLQNYPVSVGLDATNWQFYSTGLFSNCSENLINHYALAIGFDSNNNWVVQNSFGTGWGENGIIKLLPNNTCGILNQAYQIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VBV4</accession>
    <name>A0A8S1VBV4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LSDAT prokaryote domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0610066</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VBV4"/>
    <dbReference type="OrthoDB" id="310870at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041482">
        <property type="entry name" value="LSDAT_prok"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18171">
        <property type="entry name" value="LSDAT_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LSDAT prokaryote" evidence="2">
        <location>
            <begin position="93"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8174394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="308" mass="35080" checksum="C2EF9782F96BC334" modified="2022-10-12" version="1">MSEQGEEEEQQQDEQQEQQLEQQTFQLDTQIPPEDPNVFMTQQKLEQNQAWKCFDFLFPAGKLAKLIKVNYTQPKTFPMTEILTKLKLDDGLPVVNFIGCRHNFDNPNSNRGKFYAGIARACHNTDAIIIDNGITTGIEKFSLRRNCTLIGVAPEQIVQYPKLNPTKIEKNELSNGHTHIFLIGGENVDLGTEALFKINLCKAIATGKISKTQGRPRCKIVNILFADSNDCFDEIREAIINKLPIIVVRGSHLCNQFIDQEKILDAEFEDIMTDKDGFFFPLKKLDSEVIAQMVHYLLTYTPSKPKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VCA4</accession>
    <name>A0A8S1VCA4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0570128</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VCA4"/>
    <dbReference type="OrthoDB" id="296137at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8172506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="81" mass="9808" checksum="C6AEB0450431E6F1" modified="2022-10-12" version="1">MGCRVTKNKKQLKDLDYFMNILDKNKTDDETKIQEKNKLFQITKNPIFQRRLSQKKENSLTKQTYSYLERNNLTLRACEYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VD26</accession>
    <name>A0A8S1VD26_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0610230</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VD26"/>
    <dbReference type="OrthoDB" id="296353at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="253"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8174687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="368" mass="42298" checksum="1B2CF969B4A92200" modified="2022-10-12" version="1">MIVALFITTALAIQMNEQATLTTEAQTAVRQFLQSGPLGKMMFEFAQLEMHASGAVDILIEVLKAFGRQQEDRLNEENTIWQNRLGDHNARRIQYESLVTEGGLAINRDRKKLTQEYEPRKEELNEILTTLKTNMGKNEVAKKNAEALRAEQNSQFQQQANEITEAIKATDDTLEYIKRCSQGSFIEVDKINDHLLFLQKKSSKSTDYIGSIIQALMQISEKQNFADREVQKQLVDLLQSLRSYFVETLQAAYNDEDQQKLLHENRMNQLENEKQVFEKQYSDAYAEREQRSLQIEDTNRLLDTRGKELQGYQDRLTTENNNFSQNQKIHDDLVAAISGEIGFIEKALDVLMTQAFSDELQSAINKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VDE6</accession>
    <name>A0A8S1VDE6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0630213</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VDE6"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="28"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8175708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="163" mass="19512" checksum="73D726941DD8F045" modified="2022-10-12" version="1">MYNNHQIFSKNYQILIYKSLQVEKIKYLIQIINNVEELINQAKAYQRQSNLFEQIVTLYQQQTNKIDQIQQNTQNWTKDQIIKSGSINASFQNLSQIFNDYTITIDNKSKQQNFVIFNNLRQIYSMQQVRNNNLEVEDANLLIQISMSSDQKQQEKFQKYIKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VDX9</accession>
    <name>A0A8S1VDX9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0610275</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VDX9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8174771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="122" mass="14848" checksum="98CE3F8B9F5C5722" modified="2022-10-12" version="1">MFNQDLENTLFQKYDLQHQYQLSNLFNHIDEIEKNVVFQLIFQINLSIILSINWRNQNQIYLSKEQIFNLNSKQIILLNHPIQIVEIYYLNTHSKIFKWTSRLNIKTKLRHKFKLIELLSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VEQ3</accession>
    <name>A0A8S1VEQ3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0630005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VEQ3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8175337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="335" mass="39927" checksum="48BBF9E5EE4CF3C9" modified="2022-10-12" version="1">MHFDNKNMALSHLFSEAHYSIEAEKLFDKLDKKSPNQLTCSEFQMLQSSKKCLYFQYSSYFTEFARMELGNILTYLKKMLQYNLDYLQIELLFQATSQLNNTILTVNYIYFIQIQLSGPTVQFYNFYIFCQNLNCKLYLEIVLNGDIKKKTFIQGFLYLQDFIKITIKDFFVIKSSSIDISQLNRVSLNLEDFIIQDSDSDIYRFQIFIQDPKNININNFQHSKIILADNLVLLSCYLWIQNLFKYRIKIEFYNLFRLLVISIFQTKILQYSLERLSSLSIMMVQKLHLYNSLITHNSPNVGRGLYLEKQQLNDPKILNKLFKKQQSLEIWRQFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VIF5</accession>
    <name>A0A8S1VIF5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ShKT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0640181</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8176125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="284602at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002895">
        <property type="entry name" value="Paramecium_SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003582">
        <property type="entry name" value="ShKT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01508">
        <property type="entry name" value="Paramecium_SA"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="SMART" id="SM00639">
        <property type="entry name" value="PSA"/>
        <property type="match status" value="26"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51670">
        <property type="entry name" value="SHKT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ShKT" evidence="2">
        <location>
            <begin position="1604"/>
            <end position="1644"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8176125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2131" mass="240859" checksum="AA490804DE16F605" modified="2022-10-12" version="1">MIIILTLIQVISSQYCNCGQLKSQSDCKLNTKCQWNNNSCKQNQNQTIGTYCRQFDQQKCSTIIGCMYYESQCQQFTGCTSFEYNTNELCKSVSLNCISNGEECIDIGECVSYLQKSICKVDQRNQLCYWDGKACSLADTCSKLPYNTDELCRKAKSTCTVNFAETGCMDGGNCKDMQKQSQCFWNKSQTIKCKWISGKCYDYICMNAPITMTTDAECEAFLQGCTTQKGGGCTIRTTCAKAQNENACIIDVNGAKCTWMDTYCSDRFCSQGSKQFTTNSQCDTFLKGCIAKPQGGCVQNRECDAALSQETCQYDINGKQCIWNKTICQLKICKNAPIIENIDHNYCEQFLTSCTMVDGQCTTKTCDNASVDIKNCRQYLNGCISKLNGGCKIVTTCASIINQDDCLYDSKNQQCLWFNEKCIDQQCNAIQKLTNQECNSILQNCINDKNGNCLDYQCQNVWNQDLCTVDYYQQKCTWKSVCYLKTCYNAPKTLNTHELCNNYLSNCTVNKDKKGCMEITNSCEAYKIQESCIKKLDNTECEWYNDQCLSKSCETADKTKYVSMELCDQYLSGCVVSPSLKGCMTLPEKCQLRLIAELCLFDNSCYWYEDACIEKSKTCENTPKPYCNLGCVWSNEKCVQNLCNVIPNKSHQMCNAINNTCTVNDNRSACITLLQKCSDYKKNYQCFLSKTQKCYWNGTECKEYQCSDIPDDTTNFDTFEKCQEISNTCTVIPLLNEIGCLNRLSKCELYTQNQCTYTLEDKQCVWLDNLCQQLDKLNCSSIVDYSNCDSVLFQCKQQGEICVNKLCNEIEVEDFSLTFSKCQNYDQNCSIHASLNNCIDIQNDCNQYLIEEHCIQSKTSMCHWFNDKCANRNLIYGCSTVKLETYTDNKCKQFKQFCKLNSTSNGCADTGCVLYYGLTSLADCEAVDNSCTVNSARTGCTTKVQLCQSLDQDNCIKAQEGFCAWVESTCQLFKDNNCSQITLFSYTHSFCNQVSTTCTVNASQTQCITMTDECSLYSQSDCFQSKNSFCFYNGTTCTNLSTITNCSEIKVTTKSICDRYDGSQNPLATCTFISGNTNCTSRTCQNYPDNKFTHQNCSTWLATCTQNVAKTSCITMEKSCDLYLETSCFKSQDSYCQFNGTCLNVSSTCSQIASDERCSQNQVCMINNTSKCVDKTCENYNSTNYNHNVCSSFLTSCTVLKDLSKCMTQLDDCIKYLIENECTKSLQGDCVWFENKCQVATCDTVTDCNSYNCIVKKQAGCTNLLENCSLYTTQSQCKKSINKICYWNGSICSDLNCENLNGIGFNSYNDCNTILNSCTIDGSKVQQCMTKKECSQYQYDIECIIDIQNKECYWKNKTCYLKSCNVIELNQYNHINCMDQIINDKQNICTINDNGNGCMELKQCSSYKSEKNCIIDITGQFCGWKNNICNVKSCDTAPINLSTHDDCQKYLKGCTINSTNDGCIKIPEQCSLMIESQCKTENCTWQDSKCQDKTCQNAPQDLISESQCNAYLKNCGFVNNQKCFQFQCEDVELKNHQECYDNYKCTSNGFRCTAIQEQCYYYTTQISCVIDASKSLCTWVEKSLQCQTRKCSNAPSNLITNESCNNYYPNCTTKAEGGCQELQELCKSYTVKESCMKTINNILCVWDTNICRDKSCIDLFGTTHAQCYGQDSNCTIGLQGKCAIQNSCSTKLNQETCIVGTDGPCLWIINNNNGQCYQYNDCTSIKWKTDQECKLISPLCTTNGENCIAIQKCSLNKITGCYTGIDGICILTIDSKNIKSCQLFKNCNQAKFTTHEECQLANKNCTSNTIDSCIALNEKCSQYQLIGQCKQNSKGIQYTDDNQINSTGICVWESGLCRDQSCQDLKGNNHEECSKQLIQCTYNSIGCISIAKCSQYQDQNICITAVASDGICIWDKNICRIKLCTDIFEPKSQFDCQSNINSCIYDEVNKICIPKSNCSSYQSESICKQGSITGQCIWSSTKCIDFTQCNLANSLESVCNSNTFCNWVTKVSNQQNSTNTTTQYCENYNCTTWFNNKQKCEPFLSFDKSTYNYCNMSNNTCISINVSQFDQSNCYQFSKNTYTWDQTLQKCVICHQPIQNSNNNTNNTNSTIDQQAFIFKFYFLLILIIFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VIN1</accession>
    <name>A0A8S1VIN1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TRAFD1/XAF1 zinc finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0630152</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VIN1"/>
    <dbReference type="OrthoDB" id="300935at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049439">
        <property type="entry name" value="TRAFD1-XIAF1_Znf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21366">
        <property type="entry name" value="TRAFD1-XIAF1_ZnF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23580">
        <property type="entry name" value="Znf_XAF1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRAFD1/XAF1 zinc finger" evidence="3">
        <location>
            <begin position="94"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="138"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="435"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="487"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="530"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="585"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="206"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="138"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="151"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="493"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="536"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="588"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8175629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="608" mass="73956" checksum="69F48D019BA8EB9B" modified="2022-10-12" version="1">MSQQTQQCSNCRALIADTVFGLHEIYCIRNNIKCKRCGQFFDKNDPQSHEEEFHKGIACEFCKEIFQDISKHTKCLKKPVQCIHCGLDQAKDLIFYHENICGSRTEKCDICKQYIMIRELTKHVATCVPPSTQKEIVQEKQPTQKTVENRQITQQPQQNVQPFKPDYKNFDHKYKYQVEAEQVSRPQQQLQAKHEIFGYEKPSIPYNQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQIRQPINKYEKHIEARVNNYSQNHKIPQQYYNVNQQQQQQIAVEQKSKPQDNPLQPTTNLIKRQASGNQEIKNGYPQQEKLGDKYTNQLSTQQRLPDRKLNEQQKYDPYSYLNKQQENKFYENKKLENRIIDQKPSDQNLQRQYSQQPQPTKQQQPKAVDDDIDYLALGLTREEIEQQKVYLESLQQQRKPLESQPKSRLDYYQAARAYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VJN6</accession>
    <name>A0A8S1VJN6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0660103</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000066">
        <property type="protein sequence ID" value="CAD8176981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VJN6"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8176981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="79" mass="9273" checksum="207E354B74C4F1C9" modified="2022-10-12" version="1">MYCIHLGNIIFKNISEQDQVVGLTKDDINIFKQLDTSFDFKKSTKFFKRTFSQIAKRKLFLANHKLFNVTSKLKKKDGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VJV1</accession>
    <name>A0A8S1VJV1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0690059</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VJV1"/>
    <dbReference type="OrthoDB" id="429991at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8178238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="252" mass="27624" checksum="8839E6EF94707BE0" modified="2022-10-12" version="1">MNQYQATHDSSLNTSLSKQKYSFPRARREPIQNKSATDELSYKLPQSLGTRMAGIGYGLKHDFSKDAKPVPAPNLYEIQSFIENNIDKKKGSTFAYSRDQMKAHGIWGSLNQLSPGPGRYASTNSLIDSKFTFGLKPGSLKQLNTPGPGSYEAIQITNQRGNQYISKFRSSGAAIIGKDQNRFKTLQTQNSFPEPASYDISNTGITGTGFCAKNGFKSSVLNSFPKATRKGPFDLGAKSPGPGTYKAYSEFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VKW8</accession>
    <name>A0A8S1VKW8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0660055</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000066">
        <property type="protein sequence ID" value="CAD8176895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VKW8"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000334">
        <property type="entry name" value="Heat shock factor protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF206">
        <property type="entry name" value="HSF-TYPE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00434">
        <property type="entry name" value="HSF_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="4">
        <location>
            <begin position="58"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="123"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8176895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="380" mass="45072" checksum="5845BF213E2A8423" modified="2022-10-12" version="1">MDRAKEEQNIKQKASTPSFLIKTYDILENPDYNDIISWNEEGTAFVVKNVNELAEKVLPNHFKHNNYASFVRQLNMYDFHKMKNEGGDNEFRHKYFQRGNKHLLCEIKRKQGEQVEQFDERNQNSSQNSNNSMEVDMSKLKNDYQFFLNEMLGIKGKQHELQKALILIMTQNDHLINENKLLWQEIKRIRETDERKIDTLSYLLATLITNMNQSQNQQTVQQLVGVQVPPQTQIPQQEPNNHVDQQFNNLLNLQQLNQNKPGQQKDPKQLQEHNSFLEKIMKTQQQQEQQLQSIKQDISQYDNGFIKKQCNNQFQRQPQHMDSNFMVQQNSQALIQQLMSLSQQQQSQQQQQQGQQFQNNFQNYFGQNKGNENFRGFPFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VSA3</accession>
    <name>A0A8S1VSA3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0700023</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000070">
        <property type="protein sequence ID" value="CAD8178622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VSA3"/>
    <dbReference type="OrthoDB" id="439844at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053238">
        <property type="entry name" value="RING-H2_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024766">
        <property type="entry name" value="Znf_RING_H2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14155">
        <property type="entry name" value="RING FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14155:SF631">
        <property type="entry name" value="TRANSCRIPTION FACTOR C2H2 FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12678">
        <property type="entry name" value="zf-rbx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="266"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8178622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="320" mass="37930" checksum="5BCFFB519B185929" modified="2022-10-12" version="1">MQNDAIDYLTIFTALTIFKAFCSGVHRYKVNQMPTSHYETESTLATLMKIQMQFNFLLLHCTLFFKHIDNIQEIGKFNLFLLISICLYALTLMLTDYRWLLKIYRYENHILREDYPIVEFLGIQVFMMFGCALLITIQWFTIPILSDLMYQLLCIYKIPQIIKCHQHNQMPNITYFCFLEDYCILIFFSYYRGCPINIFKLQDNLGICISVAIFICIQQCIILYQFHVKPQLYQTKSQVEEFTNEIREQNIYSNFDDQGINNNLECAICLQGIEITNPLQIQLNPQDPIVLTLCSHKFHESCLIVWLKVKKQCPVCRHQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VVX4</accession>
    <name>A0A8S1VVX4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0730077</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000073">
        <property type="protein sequence ID" value="CAD8179949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VVX4"/>
    <dbReference type="OrthoDB" id="317117at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035747791" evidence="1">
        <location>
            <begin position="19"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8179949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="105" mass="11955" checksum="95FFBA6FCA3A59A0" modified="2022-10-12" version="1">MKYILIVALLILIITARSVHKNEDDEMTRCMAKHCKKQAGGCARIQGCAENLKNCANDLEKDEDLEKFDTCLGKVPQSYSLMECILQNCSDIERKSVEQLFKKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VXG2</accession>
    <name>A0A8S1VXG2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0790031</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000079">
        <property type="protein sequence ID" value="CAD8182618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VXG2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8182618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="60" mass="7369" checksum="63AB3BA7F217AFA9" modified="2022-10-12" version="1">MKYLFGIFEWRQEIHAKQKSMEQQGFEFYSKTKKQYESSLNSGVKHFCYLLIKFCMKVQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W3R9</accession>
    <name>A0A8S1W3R9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0820134</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000082">
        <property type="protein sequence ID" value="CAD8184090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W3R9"/>
    <dbReference type="OrthoDB" id="295641at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8184090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="239" mass="28881" checksum="C9985F9DA8AB2B52" modified="2022-10-12" version="1">MQLSPQSQTIFQSLFIEKTKDQINNILQYKLSQATKARQRFVKNQIDRPNTSMVQQPNEMDFSQNQFEKHRKISMNDSQLTVVTIRKPQKIMHTIELSPQITPITSFHSQRKYRQLQMCEKSTNPTPQVHSPDNEKQFYFKQKQYRNKLQLKNSENNNIKLKQYAKEQRSNSKGVQEYIKYQDEMDEYFKDKLFQQQRQLIEKIDNFKMSTKRLDQQLEPIGRLLEQRLQKMKKKQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W6V8</accession>
    <name>A0A8S1W6V8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0840158</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the flagellar radial spoke RSP3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8185091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W6V8"/>
    <dbReference type="OrthoDB" id="313308at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009290">
        <property type="entry name" value="Radial_spoke_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21648">
        <property type="entry name" value="FLAGELLAR RADIAL SPOKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21648:SF0">
        <property type="entry name" value="RADIAL SPOKE HEAD PROTEIN 3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06098">
        <property type="entry name" value="Radial_spoke_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0282">Flagellum</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="14"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="77"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="352"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="352"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="387"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8185091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="398" mass="47327" checksum="65D314AF12D03016" modified="2022-10-12" version="1">MAQAQALAGENYEFKAAPEVIKPKPKYRQQPLQSSEDQKNSSLLGCNIMYDKRVVRGNTYASVQTQGDKKLMTLKSFKKPQQKATQQPQTTYLHPSTPRPFQNRVNIDIQTDEYLEILTDKPPEQEMDAQTDYYIDKPPDRLFVPKKNGIDKETQIWEGDLFDFDQEVEPILQVLMNKILEQSRMEVLEEEEIKLMKEQQKQHERQKTSVLSEMQRLEAVQQRLDQEDARRKLQYETFLKIQKQSHQKIVCRNLSKSLINPLKQNIIKKLQDLGVFRDPLETALLYEYIPWVYDNVLSQLVDENDNSTNFNNMLNNIEENITIEHSNSLKRRQQHIDDKYEEIARKKREKIEKKRMEEERRLKEIEDQRLAEEEAVRQAEQEKLDQQQENQQQQQFQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WA96</accession>
    <name>A0A8S1WA96_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0880074</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8186954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WA96"/>
    <dbReference type="OrthoDB" id="310600at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021258">
        <property type="entry name" value="Epiplasmin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10992">
        <property type="entry name" value="Epiplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="268"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8186954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="299" mass="34393" checksum="68F605FAB60B0036" modified="2022-10-12" version="1">MSNIPQPQHPQQAPQAPQPYYGQPSYGQPTYAQPSYAQPYGAPLSPLRYSYAPPVVQQVVPQTYVPQQVVAQPVIAQQVVAQPVVAQSVVAQPTIKGESRIEYVPYEKTVLEYEEVRQRIQVPREKYVTDYYAVEYQTEYVPQVFQEKFTEYVPVDRYQERVEYYPVERQVVHQQQVQQVVAQPVQQVVTQSVVQPVQYAPQQVQYVQQPVQYAPQPVQYVQQPVQYAPAPLQQTTYVPPPVASLPLAQTQVPTRTVPQARPQQPLDRTQAQNPRQNPQPTQQPQQKQKSFLDRLFDRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WAB4</accession>
    <name>A0A8S1WAB4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0850033</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WAB4"/>
    <dbReference type="OrthoDB" id="312892at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047126">
        <property type="entry name" value="RNF141-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109">
        <property type="entry name" value="RING FINGER PROTEIN 141-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109:SF5">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="2">
        <location>
            <begin position="54"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8185335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="119" mass="13588" checksum="8DFE3B82B68EFD73" modified="2022-10-12" version="1">MIPQVGTSENEVFMNEGIPKYLMKISSTYFQQTDKPFEILSTSPRKSNDAEMKCLICFENESGYVLMNCGHGGLCLKCASNLLLKNKECYLCRQPIMKVFQIQKNNFNFVEVIGIIETQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WL38</accession>
    <name>A0A8S1WL38_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0960039</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000096">
        <property type="protein sequence ID" value="CAD8190283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WL38"/>
    <dbReference type="OrthoDB" id="2017782at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050498">
        <property type="entry name" value="Ycf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE SPINDLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="7"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="41"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="75"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="109"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="165"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="199"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="291"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8190283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="623" mass="73103" checksum="033DF39679474E30" modified="2022-10-12" version="1">MNQSIFAYGYLNNGLVYQKQGKFEKAIIEYNKAIEESPSYAAAFQNRANAFSSLMNFEEAFKDYSIAIRINPQYSAAYFNRGLLHGKQGKFEEAIMDYTEYIKMVPDNAQAYNNRANAYQNLGNFYEAIRDYSKSIEINPQYAAAYNNRAIEDCSKAVELQMVNADAFYIRGNAYKNKGKLQEAIIDYSKAVEINSQHSAAHYNRGLIYSDQKIFDQAIQDYSKAIGISPANPLYQFSLGFLQFSLKQFNQAYQYFESALECSLKLTLQQRLQFQLSKDKMMFLHQKVEILSSINQELQIFKEALKDLQRNNKISQIQDQQYQFVINNIENQFLQIVPSQLNENQVETLKKLKFYKEKIKGLQKNIYKQKENQNQDLEPLTQNIKQINSLQLTQMSRQNQDLYELKKQLSSDQNLYYRSLYWHLFNYFYAINLISKDLQQINIQSHSVLGLEILQKIEKLKNQVEESESIIIQIFELLNSALDQVINTNKNQFQSRISCIKQILQVFIKFPSEYESEIDSASLFLANNINFYFEKDNVNIFNIQIERISILEFDFEKYSENQFWKTGILHTLIILNYLIENFKQILIQSSFCSFKDVMVRSIIEFNSELFSKNQKEQQEGFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WLH1</accession>
    <name>A0A8S1WLH1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0970122</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000097">
        <property type="protein sequence ID" value="CAD8190798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WLH1"/>
    <dbReference type="OrthoDB" id="291966at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8190798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="292" mass="34804" checksum="5F7D7535B356E232" modified="2022-10-12" version="1">MDSKQNMISFLTELKSKDITDETFDWLENYLNKGEQSIIHELILNIGDHFDDQQYVLNAFQGLIKIYDINPNILVKEVKETKMTAKILVHYICEIEYNKWNESGLQLLVISFDDNHYHHQIKQLNNKQQLQLVNNILNYFGQSDNAEIFSVLIQILFVFHDNQDIVLIIIQHQNARFFQEFLLEYLNHTKNDVSKPLFFIETIMKLDKDFFYVNDFKILQQISIRQIHDGTENERRLYLRNLKIIIQYGNHENILGDEILEAARMVQMTYTDNYCLKKCISIIDNLMNKQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WQD8</accession>
    <name>A0A8S1WQD8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0970022</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000097">
        <property type="protein sequence ID" value="CAD8190605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WQD8"/>
    <dbReference type="OrthoDB" id="291106at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="347"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="425"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="4">
        <location>
            <begin position="525"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="5">
        <location>
            <begin position="661"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="748"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="891"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="761"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="909"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="939"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="955"/>
            <end position="976"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8190605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1132" mass="132258" checksum="D67478420A5778F6" modified="2022-10-12" version="1">MSLSDLLVDYGESSHSQFGKSLNNLLEDQTKKQFTGPAKFATKIDPLQEGDYTIKPPSMTSFHKVTDNIHPSLSPNSGMIQFSPKTSIIEEKNLMNPQNKEQIKEIGQTTAMTQKEGMNNFIKLVLAKSYFNRFVENMLQKSYVKKLSHFNIYQNTMLDDLRYIDSKHKIHKNLISKICKKLRFVPIFDQSSYLVIGWQLLHILTIIIIFFWTPFNISFGITFDQVVFGTLTVREVEFYFLFSIVVDAFIVINTSYIEKGIIIKQRGKIFINYVNTQGIYDLCSFASMLVAQQFNVDSSNEKIGWQLIPYTIYYCSRQFKLQARVHKLEEFFNFSGWVQDLIELIKLLFMVVYVGHLFACLWHGVAFYQIGFRKTWLETYDVATQDIFTKYNYAFYWSVQTMITVGYGDLTPQNNYERLCANMSMFLACGVFAFSFNSIGLMLSNLNSRQVLYKRSTNLLNSFLTKNQIKIELQSRIRNYYDYIFQEEQEINDEEVSQITTKLSSSLQEELNFEIRFNVMKTNKVLTKFSQKTLRQLSLLIEEVRYSPEDQILLQGTQDDCSLYIITKGTVSVLFQDEPQGRNTRVLSYLEKGESFGEYSFFTGLNRCASAKSIGFSRAYKIPRQSLLTVLQTNQIDLERFCEVRDSILLGENYQPAKLSCYSCKKFNHLIKDCPVLHYVPDKERVIKKEYFPFCQERNLTYKRKRTDRNYYATLLEMKKTIAYIRDFQTRENQADNITIMDEDLDVSYEENDGSPNEQDYSSLSKSYSKISRQQSQNRSYSQYDNNNNNNTKNLQPIQESDDSADSPSPNKRRNMQIKQTLQTAGFGAQDSFQNKIRNDDQDQNLTSFGNATPSIKDENRYTIKNDSLVLPNQIDIQARMNKKRITLVRKQKREESYKPFSSQTKLDINQEREKSLPKKSKMKDPLIDIPVIPSIQSVEHKRDSIDRKDDRRDQKRRTTKTKTNRSRTAKTHKTTKLVQETPTQEISIPVFERQAMSMELEDFEVLKNFSHYFSWNNAKVVVARTMKILLKNLEKRRQNVLQFSFYTFNSLAITKISRIKRKLKLIEDPPLEKKSVHPLNKTIKGRRTSKRSTQADDITGLFQKKPQFGTQIQVLQGISLSRFSRNDLDFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WRN3</accession>
    <name>A0A8S1WRN3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0990027</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000099">
        <property type="protein sequence ID" value="CAD8191440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WRN3"/>
    <dbReference type="OrthoDB" id="293086at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035906914" evidence="2">
        <location>
            <begin position="16"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="51"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="280"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8191440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="363" mass="40753" checksum="EA867CE754CED807" modified="2022-10-12" version="1">MKHILVLALIAFTYATTLTEISERLSQYGDHPFGKSMVNLVTVNMKTGGSLNELKQLLQQIKDELIALTQLQDSENGTFTRRSQVDLAKLQATLEQAQADLDNQRQEQSSLSNELATLQTRVKEDQAALDRNSRGSGDAQSRLDAENADFATKYSDYNDAILACKEAQRLLMNLRGEGASLIQLTQDTKSNLIQTKENFQKIKEILEAHTKKSSLTLFQPIIEGLAEMTTKVNPETLNNVLSLVARLITALQEGQDQLESNHKTQVENLTRLGDDLRNEKQTLQVSLATANNRLKEIQSRLNELDGLINISNALVEVTQLNIQDAIKINELEDAEYSNQKVSRQTEIDIVDRLIEYINQKLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WTJ2</accession>
    <name>A0A8S1WTJ2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1000179</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the strumpellin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000100">
        <property type="protein sequence ID" value="CAD8192107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WTJ2"/>
    <dbReference type="OrthoDB" id="565118at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071203">
        <property type="term" value="C:WASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140285">
        <property type="term" value="P:endosome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051125">
        <property type="term" value="P:regulation of actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019393">
        <property type="entry name" value="WASH_strumpellin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15691">
        <property type="entry name" value="WASH COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15691:SF6">
        <property type="entry name" value="WASH COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10266">
        <property type="entry name" value="Strumpellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8192107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1151" mass="134134" checksum="E68B5E36697FD60F" modified="2022-10-12" version="1">MDFLSPDNACGQSLLILVARGSAILAEIQRLSENIPKIITSKAQEYKKYEKILFDFEYLKNIDVLEDEIQRNQEINELDENFKKSYLPLVERFYRLFESIYLYYSDLMAFIQELEEAVFMQYSVETVLQDPEGKRLFAESFYLYGCMLLLMDILIPGKAREKLIISYYRYKGGQTAIEFINEVTKLCAETGYQKNGARPAFYPEEYMARFQLPEKLITEVINSIKDEDIYKYTSIYPSADHRSIALSVQGSMLYVLLFFTPTILNNKKAKMREIVDKHFYDQWVISFYLGYFVDLSEQWNSYPAAREAIKNTINFDYIQELGTFYQTKLKEQINKVQEYLYEGQMTQEFVLDKISSLMNCVRESNIAIRWILLHSNTSISKAKDIFNKIDKQEVLQLLLQSSKFENRLLKAIKAIIDNKQQYWDEDKVKCVERMQELADYFSGKALGKVKADESYMQWFTEMGNQIQSLNYSDSTHAGRKIQRLIQALEDIEQYPQISTSIQVKHYLNETRKDLTHMVKIVHIKSTLPSHISYIADFSYSWQCLKGYKDLMQAKVQSNPHSALSLKTTFQKLSSILNTPTIRIIQAGSPDLNSVTKYYSGELVKFVKEVLQIIPIQVFEVLQDIITLITSVLKPLPSRINKIELKDYAQYEDRTQIAKLTNSISVFTESILTVDPYLLGSIEVNPREMLDQGIRKELMALIHKILDSQLIFPKKDVSDFQTKLSKLGEEINGFKLAMEQIQDFVNAQGLKMFTEEFNRLIECYTSMELKGLITGNKEYEELSYDEDIPMPDEKSKQYGAINFIGRLLNQIILITNPKEIVFVESTLGFYDSNGRDVLNLKTIGLILKCIGVSGLNGIDLLISQHLSKTVKDIVKILKTELNQENRNIIDTLIKQVRLFTNYDENYDKVYKQLTKQFRPLISKILYPLQQIGQFINLRKLICLVLQMKAKVGSTKLFLCLETLNNTIINDFINRQYDSTMESDIKTQMENNLLFDLTKLFNYVGLSDPIRKIYALCEMPEHTPLILALVILHQLPLMDFDQKLNCLVKKAKDNQLQYEPNCLLMGIVGFLNQFNQLHSSLFVSYLGFYIKSTIFFMLQSKELQKQPEILGDFFMLLTVIEEIFRIREENYENFKQLIPFGLFNYLKREMIKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WUF5</accession>
    <name>A0A8S1WUF5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T1040073</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WUF5"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05599">
        <property type="entry name" value="STKc_NDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000570">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000192">
        <property type="entry name" value="Serine/threonine-protein kinase cot-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050839">
        <property type="entry name" value="Rho-assoc_Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988:SF76">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_135, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988">
        <property type="entry name" value="MYOTONIC DYSTROPHY S/T KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="83"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="13">
        <location>
            <begin position="362"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="441"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="493"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="451"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="502"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8193413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="512" mass="59939" checksum="E8F8B21FBEB02F25" modified="2022-10-12" version="1">MAEVKLSNQTKDRVAACKSYIERKYQLSLQQEMEKKMIWQQLQQNMQNLNFTPIEQELIKKEILHKEAIQLRKKRQKITVMDFEPIAIIGRGAFGEVRVCRDKETKEIVAIKKMKKSEMIFKNQLGHIRAERDILVQANCPWVVQLKYSFQDEKYLYLVMEFLSGGDFMTLLIKKDIIPEKEAKFYTAEIVLAIEAVHKLNYIHRDLKPDNILIDENGHLKLSDFGLCKHLGNKQNEILSIPYTERKQDAQQQQTRRQLAFSTVGTPDYIAPEVFSQKGYNDLVDWWSVGVILFEMVIGYPPFYSDTPQKTCQKIVNWKKHFAIPKEPKISPACADLIKRLMADSSERLGDVNLIKKHPFFNGIDWENMRNLKAPYIPDKSKITANFDKFDEQDPWYQTQEKRGREQNHFQGYTFKRSHENEVSPIKRALEELENIKPSGIRANFDKKQRSQSPVYNASPLLNQGQRSQSPSIKEQLKQTYVQYFGQYLSPLNKQKRPETAQIKQQIANKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WY41</accession>
    <name>A0A8S1WY41_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1040127</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WY41"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:001087">
        <property type="entry name" value="Heat shock transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF206">
        <property type="entry name" value="HSF-TYPE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="3">
        <location>
            <begin position="8"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8193521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="216" mass="26184" checksum="D6F2C83154796861" modified="2022-10-12" version="1">MSSKRRVGIAHFLLRTFEMLDTPKLKHLIEWSPCGEMFYIKNSREFANKVLPQYFRHRNFQSFLRQLNMYNFVKKRLKNGWNQFQHKYFKREAKNLLINVHRRSNGQQSDEEEQTDQQDSIKKQQEQLQQIKYLQNDIVLTSSFLYQQSVILQSNFQRLKNKLLYQYIEEQKQEKFLFNTFSLLNKSQTDSPKLTQTILTQNQKETDQEPQLFVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WYI8</accession>
    <name>A0A8S1WYI8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1040205</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WYI8"/>
    <dbReference type="OrthoDB" id="20524at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001344">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="28"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8193651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="400" mass="46705" checksum="AAFAB37D2EE62301" modified="2022-10-12" version="1">MLEILLSPKNIQKPSKQLPKKIINDFVYHLEDCLGQGEYSSVFKGQDRRSGQEVAIKVIENSKLNSNFSRQMLSNEIESLKKLNSPYILQYINYIYTPNNQYIITEYCNEGELKFVKNRSDQQLLRIFHQLLQALKELKSKNIIHRDIKPPNIMMHNCITKLADFGFSVNMNSLELQKSSFGTPLYMAPESLLNDMYSFQSDVWSVGITMYELIYGNVPFYHSKESELKKIIRDYVNNPILRIPQSIFIPLLKGMLDPNPKTRFTVEQLLSMISIDQNTQNISQSEINFLSNFAQNLQHFETNINDICLRWAILKILATQFKMNGNTQSQINQQINQIQNTTNFYMIKEQKMIDLINSKSDAQFVNILRQILQQKVNCQQYKPIVQDILQCDSILFHLFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X0F2</accession>
    <name>A0A8S1X0F2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphodiesterase</fullName>
            <ecNumber evidence="1">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1100134</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="1">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000110">
        <property type="protein sequence ID" value="CAD8195778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X0F2"/>
    <dbReference type="OrthoDB" id="74705at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="2">
        <location>
            <begin position="642"/>
            <end position="975"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8195778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="981" mass="115995" checksum="4B9F675B1E43CDB0" modified="2022-10-12" version="1">MNQQLGSYVKFFHIIRELGDSFQIDSKQKQILKVNFTIKDKKLMTILNDLNDEDQEEIKFSLLNYLNELPKEKQGVSRQMKRTRSSITDVDLIKGIQFDETKSHDSVNDMEKAFKLINDFYNKKKEGQTINPKINQIFENIRNLFIQNESQQNQSPILLTKGSSSPTMDMNQLIESDLLDSYKNIKFQFANYMKSHLLLMDENVSYTTIFNLLRELTKNLLNCDHFSFFNLNSNSELELYQTKTDIFDQIPLDQSITNEIASFPQNKIFRFPLNSQLCSKLDQMNYLSSYGLYSNNIIFIYHSKQNKQLDSGSILKIANDYQLIDELKNMSMFLIHTIQNAKVQFFSPLSIADMILDLGISFVRASKFILIEQMYNILSQMFKIDKLQTDQQEISVWDSESVYIVFKDYQVTVCFRIYQMNLQKQSDQRIYSEVKHYYEKYLRFIRECFDKSAFYKFFLRSNDSLVFEFDKSGHLLFLSRPIPKQIKSNFNIQFNSHMIHNNQLSYNKIFDQNVVSNIENYLQDHNWKTMKDNEKQYEIFLKIEEKTYKGFALIFTENTKGWVKKQFKQLESGNQLDSKIKAKIRKQLIQHETINFVNKLEENNPEVKNSVASLYMPLQQLILKRTKSNQTNQQDQHKIQTSEIEFKKSKFANIEELDLNEFQLENQDDLIDTFDFNIMDLTNQKEKHRVVWVILKKNAIFDDFHLPYENLQNFIKEIEYHYNINNNPYHNYDHGITVMQTAHYFCKELEQTSKNTIIDNFNKFILMISSFGHDVGHTGRTNVFEINSLSDLAIRYHDKSVLEQHHAALTIQILKYPQCNILINLNQIQFRNFRKGLISNILSTDMTEHFTLLKDFENRPKNFNDSKILSGYIMHASDFGGAGKRSNISVKWSQRVNQEFSIQYKLEGELGYPQQPYMKDLHIPQVMAKSEVGFLKVIVRPCYVLLSEFLEGRLKHCIGYIDDTIQHWEKIGKKNEEQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X4L9</accession>
    <name>A0A8S1X4L9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1110046</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000111">
        <property type="protein sequence ID" value="CAD8195952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X4L9"/>
    <dbReference type="OrthoDB" id="300484at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8195952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="315" mass="36746" checksum="8197EE0B38696441" modified="2022-10-12" version="1">MEFYILILLKSTQNGYASYHFDIKIKSNKLLQLEIKEQVNINQLKKCWMLKVKQMTEILRQLIFLYRIYGQGIANIKQSIIGFKSMPFIPQSDIYVIMFSLHQQQSMFNALHVWFQLSSQKQSAQFIFLGSDSGQLRQCGSYNEIIKIIKNKMIRSSPNLEQTLNESSLKEIRKTIAYVDDFIEESLPENVQYLEIMDYQYQAFKRVLINSVQNVISIGRQQLYSISAPISPNIMPQLQKSVSEFLQKTEYIDKQDNLKSVKEKRQSEIIQTIKIDYSLHIQPIQTTMNPGFLTLEQSSCSQQVQKETGFCCQLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X4R0</accession>
    <name>A0A8S1X4R0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1100057</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000110">
        <property type="protein sequence ID" value="CAD8195655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X4R0"/>
    <dbReference type="OrthoDB" id="3881at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01791">
        <property type="entry name" value="Ubl_UBL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000052">
        <property type="entry name" value="Ubiquitin-like protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039732">
        <property type="entry name" value="Hub1/Ubl5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13042">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="3">
        <location>
            <begin position="118"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="22"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8195655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="189" mass="22351" checksum="196EE65870E9FD24" modified="2022-10-12" version="1">MGKYKSRSRSRSHKKKKHKNEKQKTKDIENESKDWRNDKIYVGQKSVQAGLDVLEIIKKERKEDEEKLEQWKKKVGYTGLKVKAPLNIYEEIMKKNIVTPVKPVNPSLLNNPQAPRMIEILVNDRLGKKERIKCCPQDKIGDLKKLIAVKIGVRPEKIRLQKQHSVFKDHITLEDYEIKHEMCLEMYYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X6Y2</accession>
    <name>A0A8S1X6Y2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1130102</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000113">
        <property type="protein sequence ID" value="CAD8196705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X6Y2"/>
    <dbReference type="OrthoDB" id="306204at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="75"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="209"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8196705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="361" mass="43244" checksum="50C823A327B18838" modified="2022-10-12" version="1">MNYQNPQQRTPKALKINIPSPILRTQLSPTQFSSPKRVVPVTEIIRIIPNDLPGTPQKESIRKAELSVPELQIFLDDLLKRYDKLAQDLQKSTQKEIQLQNEMNNLQQDTNNKLQAIYTENQILQETLKNKDQEIIILNQQIERLKEQQIKEIQDLKQENEHVLGILTDKLQQMNIFAKEKISELEQIKSLLQLRDQEVLEWRSRKNIADSNNQEINNLKFKISKLEQEYNQLIKEDQNLKSQFQQKDNEINILRNKLQQGFSQNDQQLFEELEQARQEIKYYQEQLMQYEKNQDFVDLDDQEDKIQVLQNEIKRLNKQLIDMRHELENYRIHQSENEFLKSKLSDKQNQIRDQKLRNAQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XAC1</accession>
    <name>A0A8S1XAC1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1160125</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000116">
        <property type="protein sequence ID" value="CAD8197812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XAC1"/>
    <dbReference type="OrthoDB" id="292321at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="192"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="75"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8197812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="279" mass="32655" checksum="3916E2957BE3D46D" modified="2022-10-12" version="1">MNQSEQNEEHSPHNSENASQNVSQFLQRDYYEKFKLGQNLYEQSITLSRLSLGEKTSEDYNNNTYIGLIQIQERLSDEIIKNQLLENKVTQLNKDLQAAMEHTALLQKQNENYIQMLQEANTKLQKQIRNNEIEKVLNQQLMKKNQELLEVNNNYQKQIQSIEKKKQIQNFSDCKNIILSKCHQKSNQSICSSNRNKEKQNQGNTEESTVKDSSLSDFKSNDNPRFAESAQQFYKSQNFWSPVKQSSPKAQHLNLEKRIESTLAQIRAIKLQIQQLNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XAC6</accession>
    <name>A0A8S1XAC6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1170081</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the proteasome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase T1B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000117">
        <property type="protein sequence ID" value="CAD8198057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XAC6"/>
    <dbReference type="OrthoDB" id="7854943at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03762">
        <property type="entry name" value="proteasome_beta_type_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000073">
        <property type="entry name" value="Proteasome subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF0">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE SUBUNIT HSLV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="4 5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8198057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="214" mass="23692" checksum="E64C47F388071894" modified="2022-10-12" version="1">MNYTDILTSKFEPETTGTTIMAVIYDGGLIIGADSRTSSGQFVADRCADKIDYVHDRIFCLRSGSAADTQAIAKYVRYYIDAHAQELGRLPAVATAANLFRNFLYEYKDSLSAAIIVAGWDPYKGPQIFTLPLGGSVIEQKWSIGGSGSTFIWGFCDSNYKENMTLQEARAFVLHAVAHAMYRDGSSGGIIRLLNVTKDKIEREFIDYKDVPVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XIF4</accession>
    <name>A0A8S1XIF4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Copper homeostasis protein cutC homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1250064</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000125">
        <property type="protein sequence ID" value="CAD8200654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XIF4"/>
    <dbReference type="OrthoDB" id="300369at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005627">
        <property type="entry name" value="CutC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12598">
        <property type="entry name" value="COPPER HOMEOSTASIS PROTEIN CUTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12598:SF0">
        <property type="entry name" value="COPPER HOMEOSTASIS PROTEIN CUTC HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03932">
        <property type="entry name" value="CutC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8200654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="129" mass="14508" checksum="82EC3FC133818051" modified="2022-10-12" version="1">MIEDIKICKQLQVKEIVTGILTTDSEIDIERMKILIEIASPIQVVFHMAIDDCHNYHQSLQQLINLGIKRVLTKGGKYKSALDGKDSIKQIVELFPQLTILAGSGITKDNYISLVQYCNLKEVHGTKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XL18</accession>
    <name>A0A8S1XL18_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1290037</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000129">
        <property type="protein sequence ID" value="CAD8201811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="304932at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398:SF0">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1047"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1139"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1182"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1049"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1146"/>
            <end position="1160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8201811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1412" mass="164644" checksum="FDF68D22B8868169" modified="2022-10-12" version="1">MITRMIRKMDIFGQPINLNLDLRSKYQTFLGGFVSMFIVLLLIGYSVNEIIGYTIQRGIQITQETRFNYDPDVLVLNNKNFIFAIRVEQESFYQFPQFDIQVRQHQNKKEVSMELQQCTFQQFISVLNSSQVLDFLQANQVDTWLCPKSEFSIELEGTQFCMQSYFEGKAAQFSSLMIEIGECNQRSSWGSECQTRTQKDSYKLQMITKNTFINPFNNEFVAEDYLDNSMSLYFQPGDLQRIDYQFHKHITKDYQGQIKIEGLLDEQEWNIFSTNSFKQIYEGKNNLIWSRIVFMRSNFSTNYNREYQTVTDLLSKIGGLSQSVISIVGSIIGENDQGTKGSGLVDPLHYSVQLHQETTQPLKKKRKVVDKTNIIKQPEEDSMDEDPQFKERVLRVVEEQKQIMDKYQFKSRKHFLLSTFQQTILMKQSIQLNLKYFFQKLFCKKSDPKEDPRLYVLQKALTSVRQELDVFSILNRIHQLEKLKKILLDRDQLILFNYSPKPVIAVNEKDKETDFLHYSKQQSLKQMTLVDIAEDIKKKLKSTYITKEHLSIENVFNSYKAIAAKNRKSNRYYYKINRKLKQFLGDQVKAIFKASKLLKFPEDDQIPEMGVDDIMSVGGETVAQYPLNIENLNYIQFQFNQALRLGKYIYYIENSLLRKVSFIENNCSDSVIVQAEVSMICTDGKLIYSLLQNGDIYQIEQPPRLLKSGNNYKWISPIAAIDQSHRGFIWKTNKQIYSHCKHISSYQDRITLITIGGKIFNVVADKTIAIPQLNGLKLNTYFKKSLLFSFGGVAITESGEFYLFNQKMIRVKIKDIKDIFSTDKYIFAIQNNHTILQYSIEDIQSHQFFTNQQIFQKMHYCNDVEFKCKQKQFQNISILQSEYSCSSFAYIKENKILDTSLNLTDKTNQVQNKSNSILIMKQSLFDDWDCKKKILDSRRSTTIDKQPQTECINEGSTLTLAQKLATIRESLNFEKPIPIQIEKDKKDRNRQQSLIQLHQVQIEDLPQNPTPIKLDNLLNKIHVNKPEKPIDQLLLIQNKQELQDSLPTFQNDNQFTNNNDTLNNKKKSKQNNCTSRSNRRQDSMSAQSGIEFQNNSGFIQQQDSFVDILDNDSIKKDLPLQLQPFDKSNKYKKNTLDTIQSYDNEQDPKTTNRLSNKKDQVLQLSNRSPNKKTIRDPLLTERSSTKRNIEQAKQDLETLVNMINKQKENTEISKSNSIVHVKQKSSIRMISPPIQVGSSLTRPESKITPLKNRRASLISESSQQESFKMPQRISKQNISSKLNTQQSFNSSSQHSQFQFDSQNFRQLNTLQDQESQLDLKSAKTIYDQSLTQQMKKLDQLKGLKKVQCSSQVNILRNNSLNKKSSIIVNTQQKQCKQNLRKQEAILKKLFFRLDVRLKLQQLEFVNNLKCLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XL95</accession>
    <name>A0A8S1XL95_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UDENN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1290111</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000129">
        <property type="protein sequence ID" value="CAD8201945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XL95"/>
    <dbReference type="OrthoDB" id="291879at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051942">
        <property type="entry name" value="DENN_domain_containing_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15288">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15288:SF0">
        <property type="entry name" value="UDENN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00799">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="2">
        <location>
            <begin position="307"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8201945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="772" mass="90776" checksum="598E5A5233A0A1E1" modified="2022-10-12" version="1">MQTSHPSPVDKDKKIKIMDELIKTNQKHIDHLKQTITQLKRQLQFEQKRNQDLEKDNTILKDQIKKLHSKPTQHTQFTQTQSEVHQSKQLSTKEDQQLDSATKNTQKPDNDPNNLKQQILNQVSPSKQDIKKIPKLDLKIDINSALQRQQSGPISSVKKSQTYLHGLTNSKLIDAIKERIPEKTNRISQVTGEKNNSSLRKSLLFNFQNNKSTIIDSENIEEQDIQKRRNSDGRQQQFFESIEENRVETMEVSALQQPAYKLYEKFYIMGCEKKEFLEFDNEPNIKEGILPANILFKIDSMNTPVYEEIINGFVYPFGNQVEKIKVNDSFRKLKEIMYSGNKYELLDKFSLFTIKNQDTTEDFSEHINQNLMNQANPEKLLYGICLSVYDFIETTPDHIQLTFENRKKRIFWKYKKTYCFLTYFPFYELFQDLLISIINLIKINRTDRYLKKYSEEYEVLREVDGQQIILEFQDELTKFLNLIQTIKPMLGHQQLEVQTLAGTLKYRIPNQLQLDIELRIWSANVTLQILNYNEILTIFLAMISEISIIFICEKSNILTSVIHFFHHLVHPLEWTQGIIYNLPEQLLTMIQSPVPIIIGVNLTENDFNLMSLSDNCENHLFIFLDRDKETKFLSKPKNVLDQISTPSFGGLLMQIEKILKTQNNIRNPSPSIRQASNKFDDKKAIKFYFDDNDQCKSKRVLETFKKIINDYIISLLPSENEQGVKTEQGLNLPFIEHYLVQGSNIKDRKFIQNLFKTQHFLYFIQQHYCDQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XPW0</accession>
    <name>A0A8S1XPW0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">H-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1320004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000132">
        <property type="protein sequence ID" value="CAD8202708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XPW0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019019">
        <property type="entry name" value="H-type_lectin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09458">
        <property type="entry name" value="H_lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="H-type lectin" evidence="1">
        <location>
            <begin position="42"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="H-type lectin" evidence="1">
        <location>
            <begin position="320"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8202708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="708" mass="82454" checksum="EFD00EE0B45918B5" modified="2022-10-12" version="1">MKVYLIFHIVYGIITYDSNTIKNFAYQFSGGSRCISPLERQVTVYFSDIFAKPPKIILLTEVFDLDHPQVYFTLQIMTVTTDYFILQQKCDYSVVYAIVIRWQAIDDERIQVINEFNMISFQNKTYPHINPNFEEAVISLINFSYKGSIQFNILIEELTTTNVSVVITNPNDGLKNLLVLGYQVILGVKEAISQFSSIQTTAAYTSPHYNFKDSSWLITPFIGFSYNVNDNVRLNITYYKDEQAIWYQLNTFLSTIDSYDFRWEVLELLYSTKPLASMAQVYLIFHIVYGIITYDSNTIKNFAYQFSGGSRCISPLERQVTVYFSDIFAKPPKIILLTEVFDLDHPQVYFTLQIMTVTTDCINYQVLDFILQQKCDYSVVYAIVIRWQAIDDERIQVINEFNMISFQNKTYPHINPNFEEAVISLINFSYKGSIQFNILIEELTTTNVSVVITNPNDGLKNLLVLGYQVILGVKEAISQFSSIQTTAAYTSPHYNFKDSSWLITPFIGFSYNVNDNVRLNITYYKDEQAIWYQLNTFLNGKFWSYYTPQNHQPAWLKYSFTTIYTALECRTLRITQIKEKQAVFRNSFEIEILETSQLFNNQDTFQIILDKSYQLINIKIYAKCFTNKSIKSYLNKCNGCLQNQQHQFQHNCYGAINTINFSIKLFPTILAHQELIIIISNIDCQVFQVLYNQEKSQVKLIDIKQIDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XUL7</accession>
    <name>A0A8S1XUL7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1390143</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000139">
        <property type="protein sequence ID" value="CAD8205111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XUL7"/>
    <dbReference type="OrthoDB" id="284242at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8205111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="254" mass="29322" checksum="968549F1B3D4289A" modified="2022-10-12" version="1">MFSEATKEAKPDQLVPQLNQNPSSTFFVYNCYIPVVQDFMNQPPTLQVRNSSESFPQILKQTDSLQMGHLGGGMSIPSFQFGASTPFINAEDVMRNVQPQPTQNHFASFILNEQLKPQNQPQKNRIIEGTQIMCDIEIVLISRYFDMKATVFCSPNNKYNLKEDTSISNSEWLKDRMSYRYKKPIKESFCDMIEVIQKEGESNMKGIIQPIKFQQNGFDSIERQVPKSVKLEKPFSLFILEIQQLVSQFLFNTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XUN2</accession>
    <name>A0A8S1XUN2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1400050</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000140">
        <property type="protein sequence ID" value="CAD8205236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XUN2"/>
    <dbReference type="OrthoDB" id="277931at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007941">
        <property type="entry name" value="DUF726"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05277">
        <property type="entry name" value="DUF726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8205236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="66" mass="8130" checksum="8A2B331BAD2AE0EC" modified="2022-10-12" version="1">MILKLIYNSFRWIDQPCGLNEFQLGYRRIENYNMSNLIKGHKDYGIHIKQILIQTDFLQYLRFLYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y3N2</accession>
    <name>A0A8S1Y3N2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1510140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000151">
        <property type="protein sequence ID" value="CAD8208696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y3N2"/>
    <dbReference type="OrthoDB" id="288501at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8208696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="101" mass="11955" checksum="FC5A0D2EA60FE881" modified="2022-10-12" version="1">MNKQQKNRSLPPTKVNAHHKPHDFQEIENCKQAIKDIIQHTESENKPKQSQHKSVDKTQQQKKQLHSTVQEYNENVGKMFTSTKYGIQYMQKKVMHNIDQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y4Q5</accession>
    <name>A0A8S1Y4Q5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1540046</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000154">
        <property type="protein sequence ID" value="CAD8209296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y4Q5"/>
    <dbReference type="OrthoDB" id="292777at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8209296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="263" mass="31757" checksum="ABA54EF7572DA66F" modified="2022-10-12" version="1">MFRNLRQQYQKDLDYHFMKQFNKSSINLRCENHKSPFAIKKYLSKVNIAFQSQLNELSQCNDKISKFSKINYYVRSRSSQNTYEGYLNQRDQQILLSLPFHSIKMHQREGIKKLWRKYIFLVIFLLRYKKHQQEQFKLNLQTKHLQLGTPKILIDTTKKRRRNAGSQFKLSTFAEYIDNQDNKQESKTKRIEDEKFKKSPVYQSFKYNDVRICRLLRLRNNSVRTSNAESPLKINQQVPKNNKLPPLQQIDKRMQRVYSNFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y5C7</accession>
    <name>A0A8S1Y5C7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1450149</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000145">
        <property type="protein sequence ID" value="CAD8206974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y5C7"/>
    <dbReference type="OrthoDB" id="10297477at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8206974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="173" mass="20094" checksum="D9614A0D7BF0588F" modified="2022-10-12" version="1">MEQNPVLILEQTKDKTKQRKAIDQEIKTKLILSVVNDRLPIYQAAILHKVKYSSAKHILRNYFNDSANFFSTQRKRKRKILCNNVFVLIDACSGKITIKKQQPNSISCTHNGQTPGLQQKSLSQLSYAIYLEVQQLQKKFGQQTKELDCISKDVEKLYQIIKKQHNEMTNLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8E3</accession>
    <name>A0A8S1Y8E3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1550019</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000155">
        <property type="protein sequence ID" value="CAD8209518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y8E3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8209518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="43" mass="5175" checksum="6A40E668A1925FE0" modified="2022-10-12" version="1">MKRSKLLHEGKKFMTQLKVLIISEEILFEIIVFRLIRNMAKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YBD8</accession>
    <name>A0A8S1YBD8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Anaphase-promoting complex subunit 4-like WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1630056</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000163">
        <property type="protein sequence ID" value="CAD8211385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YBD8"/>
    <dbReference type="OrthoDB" id="308449at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024977">
        <property type="entry name" value="Apc4-like_WD40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050505">
        <property type="entry name" value="WDR55/POC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44019:SF8">
        <property type="entry name" value="POC1 CENTRIOLAR PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44019">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12894">
        <property type="entry name" value="ANAPC4_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Anaphase-promoting complex subunit 4-like WD40" evidence="3">
        <location>
            <begin position="91"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8211385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="338" mass="39597" checksum="F713287D291A0779" modified="2022-10-12" version="1">MESQIKKKTCKIMKIIQVSLKYSNFANDLDTKIELKRLPCLIQIIDKNKLFVGISNGDIQIFDISTVKLINNLVGHAYSVLQIQSFADQYLITLCQNKEARIWNSRNLQLIFKINQNIAVKKIYFSQDGQKVILVAYDGSVKIYQTQTGEIINQINQNLQQVQFIKYFQQIDQCLIAYQNKQERYVLLWDLKDYTYKQILQGFKKRIKDAYCNSNNFDLAVAILNSSKMIIYNILTEEIKAEIEGLNDSKINVQFTKNNHLIITDQQNILVYTDKGKFKQQLNTFIEKKAYFIKVLANQNHINNRKKFLNNDQIITENEQTNHSQFWLETPSYFAALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YBL9</accession>
    <name>A0A8S1YBL9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1580054</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000158">
        <property type="protein sequence ID" value="CAD8210197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="296458at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035733194" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1295"/>
            <end position="1318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8210197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1334" mass="156977" checksum="F656470EBB03DACD" modified="2022-10-12" version="1">MTKVQFLLTQMIILAYSCSYLNNNTIFLTGTLQEVINVPLSSIFHHDDYERITLKQPNKYLTIETPISKYSETPNNAISNSKTISQKLQKSTLSRDPLNHITILVVVNGIYYIESNIGYYQNLPVITSINQCRTKTQSICYDVLLIDKLTIAECEDEQKNSYLIIINSTKTSYLDIDKPISDFRKIDQIDNYMFRGTANKIYIYIEEENNLKYLNTLDENTLKELLKKDSFKLLIKDFQIHTNGQLSIMNAYGEIIIVQYQNNSWNLIKIIETKLNDVQGYDYNIYTNTYGIISKNKILYQNRDQEQQIADIQNQDVLYKIYITKKNILLFYNNTITLLNQKLEKIYNLKFDDEEIFINTNPNAEGFIFISKTRVAAYAINDNYSLQLSLISTISDNYQKATLIQESYPKNCEIIVFYKTEDYGYSKILQSQYSQGLIAGGINTDSNEINIRPIFQGSNLKYQFQANSLIKIEIDKHKIFQINGISENQELGYRKILKGNGEKIFYLIQEDADLQMKGYFCVGNIELSCKVLFTKDNFKKLQNSFEQLWWSNNESLFFATFQEKIITIYSLSTETNNFEILTTINLESNVKQIVTDGFHLFVLIEQQIKIYEITISDKFKLINTFDIVAQKIYASQYVKNQLFIEQEFNLYLYDIEYDLQTLIWYDTIQFEQSESNLAIFKNHFLRFTKPINKDEYIVTVFNYANKRNIYMEKQILFNYYSNIKLSTLEYSFERNLFYIHGYNKQSLSQVILVYKITSNSLDTLFYLINISTTTIFTVADTYVLNTDIVNKQIENYYITGDLLITSKLNQNYQQISYTRQIQLDFEVTNDLSNKLVQSIFINSVNRGDKILKLSDKINLEYQKNNDTIKCVYLGQDWYSGQVFDIEIVNQSTKIKFNPPLTKEEEILEFSPQIQELNSSRLVQLMSNKLNLVKKEDFTYREFKLDQAYQFTDILLIVGENIYVQAETQESIWLRIIQCKDYKDCILLDNKLKVSQHIKKVFLHQNNFFLYDGKQIQVYDTKGDQTNLEQFELFQSFPQYIYIKQMEFNHLQGNVYQFISVDNWGNPEFQMREISRTSTKNYFVRYIIDSLINEQKINLQEIQLSAGFVLRKNEITIIFKNSASYSFYYEMDCSRNELCEIAKFKFNGLYQQYKDWQLHDFYPSFQSNENILLLIYNANTHYELLIYDMETPSNNTKPKNVIARLPSPEKNLELNSQIVSFIYTFNGNLHLLTSTQNQIKLQHYSLLRSYQLCTEEITFEDSMNFKISNTDQQISIEEEITIKEYVPPTPEDKGTFPIWAIIVIILVVLLIGGGIYIYYRQKTNKKQNTNLLLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YD44</accession>
    <name>A0A8S1YD44_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1600085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000160">
        <property type="protein sequence ID" value="CAD8210727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YD44"/>
    <dbReference type="OrthoDB" id="290466at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011:SF28">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="38"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="170"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8210727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="516" mass="60578" checksum="1892D8B77E118719" modified="2022-10-12" version="1">MDKQQLFAECIRILQITPIRRSPEELNHLLIFALTITFFRELGEQSLEMLHRCIQVLSYRTIKSGDILFKVGDSGSLFYVILKGSVGINIRLPNPDDPQQFELKEVNILKAGASFGELALINDAKRTATIVAKEDCVFAVMEKHHYKSILGAQEIAKIQNKISFLCSFPFFSSWSFREIKTISYHFEPVTVTLNQAIIKQNEYCNHFYVVRDGEFQVQFSYKNKLCSICTLGPGESFGQEAFLSKEGQFLIKSSIFVQWGKTKYDIVCKSEIGVAYKITREDIQKRFWDSKTQVVFLQLLSSIHHFRSIKAEELIKEIDNKRKKLEEQIHLPIPLKKSYVQKYNLLSQRNTNDDLLKDKQYDEFKQQEKQAIEQFTKLLKDRNCSYAQVKTFFDQKKFVSQNYFKKRFYHRQFFRFASNNDKFRKSANEQELEQMNGMLTQCYNQQNELNINNSQQFNHHKVLSSPTTMGKFIKRIKLKVATHQKNEDTQTIINIRTHSPNSYRSMNSRRMKTFID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RXQ1</accession>
    <name>A0A8S1RXQ1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0010503</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RXQ1"/>
    <dbReference type="OrthoDB" id="303992at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8132222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="180" mass="20481" checksum="0CBFE5F3218A5C0B" modified="2022-10-12" version="1">MYLTTPQRYPLFKGVPSSPVMPRPFYQATSPTMSFVNPFVQQNTYRPQPSLPISQRGPIPTQNPNQRAQTPQARITGKTVPSNPPPQRVQASLPNNVNPKGQNPQTVIPNKPYTRIMPQPNNKPQSTIPTNQQQMIQSLSKENQDLKKKLAEKDLELQKLKAKEETLEQKFKELQDQQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S0D5</accession>
    <name>A0A8S1S0D5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0010504</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S0D5"/>
    <dbReference type="OrthoDB" id="293033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="58"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8132224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="313" mass="36105" checksum="441429F1CF3C8327" modified="2022-10-12" version="1">MKLISLCLISLVISNNIMLQTANHQTFQEVFRQFSKQSDFGNKLFGELSVFIQKGATLQDLNNHVEELRKHLANDQEEDEKYFNEQQQSLESGLEGLVNQVQELKESVDIAAKKISVLNQQQQIIDNLFSQDQDSYQKRLANQNVILETISLLVDRVHALSSEGHSLIEKDNLLEEIKQLGRGKHVEVLAQITAHLDTEQVEKVLNLLENLKNAIFASLEADEAKEQENIRLYNKLTGEITASLSESQRYYQQLEIHFEQKDYELSVGKESIKEVGQKLQDRNDSRQFILDRVQEAQQLLNDNLDRIARWSQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S2H8</accession>
    <name>A0A8S1S2H8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribose-5-phosphate isomerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0040318</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S2H8"/>
    <dbReference type="OrthoDB" id="2106730at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003500">
        <property type="entry name" value="RpiB_LacA_LacB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00689">
        <property type="entry name" value="rpiB_lacA_lacB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30345:SF0">
        <property type="entry name" value="DNA DAMAGE-REPAIR_TOLERATION PROTEIN DRT102"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30345">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02502">
        <property type="entry name" value="LacAB_rpiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005384">
        <property type="entry name" value="RpiB_LacA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8135621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="150" mass="16693" checksum="C39725E859865CBD" modified="2022-10-12" version="1">MNQIKIFIGNDHAALEYKNNLLEYCQKKGYQIDDKGTYTNQSCDYNDYAINVCNEVIKSNGIGILICGSGVGISMMANKVKGIRCGQVNDYDSTLKAIEIGCNIISFGARTVGIEIAKQIVETFISNQNRIINHEIEKEILSVEELNYGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S756</accession>
    <name>A0A8S1S756_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0040485</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S756"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="13"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="315"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="315"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="337"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8135908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="346" mass="40957" checksum="D22299277F646710" modified="2022-10-12" version="1">MQQEQPGSECEGYIIIKYLGYGAYVVVKLGRKGHELVAIKFLKIKNEQERPKIIQKLQQEVQILQQLQHPRIIQLKYFSDNLTYKKKNGQIVYKIGMILELGEKGSMLSYLLTLKRFDEQTSRFYFRQLMSALVYMKKHNICHLDLKPDNIVFDSDFNLKITEFGFSKQVQGSINDFFGGTKSYIAPELLDRIAYDGSKVDVFAAAQILFTMITGCFAFKNCQEQPYNLIKQNDYAQFWNFQNNSHQTLFPQGFPDDFKDLINKMFDPDFNRRITPEECLQHPWTMGVEAKLNQIQDQFNNVQERINQQLELQKEEYKRQQQRRRDQERAEQEMKNLLQQSQQSYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S8I0</accession>
    <name>A0A8S1S8I0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">V-type proton ATPase subunit a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0050105</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the vacuolar proton pump (V-ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S8I0"/>
    <dbReference type="OrthoDB" id="10264220at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016471">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026028">
        <property type="entry name" value="V-type_ATPase_116kDa_su_euka"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF63">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001293">
        <property type="entry name" value="ATP6V0A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="386"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="417"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="482"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="516"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="576"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8136348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="796" mass="93689" checksum="124B4A836D2D2E19" modified="2022-10-12" version="1">MFRSQEMSYFQLVMPQDSAWTIMDQLGYLSKVEIIDHNPNEALINRPFANYVKRCDDLIVKIENMLQVAKNLNLLSNYKNGNLQQFTNNKNSHTHTYLDKIEEDINKKTSIFQEQNKHLEQLIDQSEYIQNYIEILIESRKYLGENVFQNQQISKFEYYVGILKNLELLQFHRVIFRVTKGNSYVHLKRMNEKQSIFIVLFPNIGNYGKQKIQKIVEQVSQGKFTLPQSLLELKKKLNELKIKQAEYINLISMTQKQLCQCISSMFVIKNGLPLIEFYKFYLIKEKELYKELNKLKMHGRLFLGELWVPTKDIQQLEQTIYVIKEQQSNNPGGQLAQKNPPDFLQKPTYFKLNEFTSIFQEIVNTYGIPRYQEINPAIITIITFPFLFGVMFGDIGHGFILFMFGSYLCLFENKSFYNLRYLILLMGFFSFYSGLIYNDYLSLSLNLFKTCLRQENECVYPFGIDPMWGGHLEFNDSFKMKLSIIIAFCHMLLGISLSGLNYLFLEDWLRLSCKFIPQLLFLICTIGYMVFLIIYKWLNHLEPQNAPSIITTMISMILNLGKIQGPQMWEGNSQDYVQYCLLIITIITIPWMWFPSIISLFLNNKNLQQNEDKRKSQRIYYGQLIEESGIEMTQSHSHNHDQIYLKQNKQNAEIQDSTVHIQQKKDNSHQDIEDMIVHETIETLEFVLGVISNTASYLRLWALSLAHSQLSEVFFELLLVQPINHGQPINLMIGYPFWALITFGVLMCMDSMECFLHSLRLHWVEFQNKFFKGDGIQFKVYSFRDRIKASINQESQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S9P5</accession>
    <name>A0A8S1S9P5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0050440</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S9P5"/>
    <dbReference type="OrthoDB" id="298170at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8136976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="421" mass="49751" checksum="0AE703D9E2FE968F" modified="2022-10-12" version="1">MSIFLGLIVHDQVPRDRYIRALTEIEQLMYVHKEELVILNSDNILKFKIQNSIFWLPKNPIYLNTIYYRITGNDDLQSAIEMIWDCLCILKENFPSKLELYQENIDEFNLNEWENELLRRQLNNLNNRQSNEPLTKDEIEDVFAILNIAQQLQNIKGQEICLNLLNQVQPFFLQTLSQSYLDQLEQIAKGQHKIIANSLLNKIIKVTSNTNNQITQRSSNHDQELINVIRQNPRYEQQFSNSSIIFQMKETNEIDRICFYLDYTLCKNQILLKSIKPTIQIIIDKYYDGLYGQDQSEFTKYCVFHTLLGQTILNIQQFDNTTIQKIFVIFEEGILNWIQNLVQMSPKLIYNLFPTTLLQIYQSQQYSSQLKSHKNNIYSIICKKMGMTNNDNKSEQQKIYQNLDEIKCISPLLAQFLEQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SEJ2</accession>
    <name>A0A8S1SEJ2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0060114</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SEJ2"/>
    <dbReference type="OrthoDB" id="256303at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="568"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="613"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8137482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="823" mass="97462" checksum="4945096558C5F39A" modified="2022-10-12" version="1">MQYPFSVSEFPTNSISIISKIGSENFNQNINKSKYFLYINTVNKYIGEQNVFIQNIYSLQQVFYRIIPNFTRVNMENKDILYQLKQIKFMNKDQSEKLCLTHNEEIIALDKEQQDKKKRGLCQQCWQCLSQERRVQVVLIKDSLKQIDEYKKIIKADRENHLEINIKVLNSLIEELGQVKDKYLQMFELITVSIRKLIEQLQIQEQSLINKVDSINTIDIYNFYEQAKEEFKSKSLCFDDIKNDIQCKLLEMTETQKISKCIDILQDFNKIKKIFEEQFPNKNQDEINQHNLDITCDEHKEKIILVDLNKDRANQKRIACLLCVDNYSSQYKSIKYVQQEWNYIEQIRKENLDQIASFLKNKGEKFQEILNKMKQDSQIIIEKAQKQITTNMNQFFQEYKIQQKKFGQSWSKLSKEQLIHIANQLSQNDDCQLTEDLIQSEFKTKDSLIDEIVKETIQTLQEAQVIAIDQTFKLLRNLQNEQKSIIKDTKQLDKESKMEIKLNCNSSQMKFDISPKNYGIISQYKLIKNYICYSIAINNDSSLVLVGQGRFIYIYELKPKELKKIKCIGKHKNDVNCLNFMTLSNSFVSGSRDKKIKIWKQYKNDQWKCEQILVGHTESIVCLLINNKDQMIISGSLDKTIKFWQYTEKWLCIQTITHHSDAVYGICLNEQQNKLISCGRDKKILIIQQENNEWIVKQIIEVEICGSRICFINDTFTFQPFNHPEMHIYEFIDNQYKKTKQISIKNGSSCDILFPQKFIKQKQLLINKNGPYIHFIGVYPNSEFIVEQSIELKTEQIYGTISNDGKYLFICDDHQYRLQIWQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SGK8</accession>
    <name>A0A8S1SGK8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0080101</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SGK8"/>
    <dbReference type="OrthoDB" id="293318at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="28"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="339"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="591"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8139443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="716" mass="83969" checksum="8DE07B427986AEB3" modified="2022-10-12" version="1">MIFYKSKGGIHLTFALKLLVTASFSNDIYLIVISSVNIFTILMKMTIHKKQTLIWKYIRIILLLELCLNDQPFGPVIIASINYNIKYKIVILSFYILRASLIFFNTFKIIDWVYVGILFISFNVYFYLHDIKQSDSKVNCQSQRKLTQSEIPSQNDSNDVIDFFNYIPLGICLLDKNYKIVNSNNKTKKYCSNIEENSLQQQLFVMIHKAWIEQQQNPSALRFEKRSMFNSSEDYGSDFQLRRMKSFSQTRKKAISLTFHKQFNHIIDFAQIINKFKNSGMINNFNSLKYRDQTSQKTYEIKIYEISKGYLITIRNITKNEKQIDMKDRYHFQQMLINSFSHELRTPLNCSLALLQTLEQEVKSDLNDTYLKPAIISNKKLLHQINDILDFANFEVQTFQLRPTIFKLSNLIQTIEDYFQAECKQKKIEFIIQVCDDAFIRSDFDRILQILVNLLNNSVKFTKQKGIIKLNIQRVGSLYQFEVYDTGCGISTEKLFLINKILQNSEVDLARRGEEDYLQYVGLGLKVSSQIARQLCDNGELFITSQLNQFTNNRFIVKDLQSYDETLTIPTDECIQEPYKSYVKKKCNCQNVLIVDDIPFNHLAFITVLKYFNIKSDSAYDGQMAIDMVKSRYQKCQCTYKLIFMDIDMPGIDGYQTTKLIQQFLEQNNMNTVIIMCSAFDSKENIDIAYKSGMKDILPKPIQTNRLKKILYKYYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SLX0</accession>
    <name>A0A8S1SLX0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cilia- and flagella-associated protein 157</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0110150</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SLX0"/>
    <dbReference type="OrthoDB" id="441129at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14845">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING 166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14845:SF0">
        <property type="entry name" value="DUF4515 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="416"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="5"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="210"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8142451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="441" mass="52540" checksum="252E1BA69314A5C4" modified="2022-10-12" version="1">MPPKKKQQQEVIEFTEAEKKALEKLDHSDLLIEIQVLKDRIEELQKKIYNLNETIRTINEQNLALTLKMESDINRKESDYNALLHQYEDLKKQTTEEIESTIQRKDEKIRYLEKEKADLEMRHKDELQRINDELKKLSSFKEEKELLIREIHDLKEEMNKKDYAHLDEQSKANKNFLKQTQNLTNVNNAKIEDVQRNANKIAEEKVAAKYDGILKENEKIKSELQEYERTLSIYQKEKETFQQENINYKLNLSIKEEQAKEYQMLNHQQIIKIKQLKEKIEYLKSYIANEVTKQTKEIEIMKHQGNTKVQELEMQLRNLRHLLDQRTKELKTVKALAQMILDQRSDIEQFFLQAIDQVKNEINKKNKNQKNSRLPEISQKSQSLESNKVDINDLDLEEKEKLLRILFSKMNQGVPPTNWKSQLGHSKSQVDGSSFRDNQFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SNM0</accession>
    <name>A0A8S1SNM0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0110078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SNM0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="279"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8142315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="700" mass="83547" checksum="D088457B27207276" modified="2022-10-12" version="1">MLGQIREGKYKYVFLQSDKNDEIIVTRIMVADEVQKENKKISIEEIEQNQRQNLKNKENTICNLKEIKKKNNQIQENQFKFMESIFQQDDKYCNSLKKQFKKTILSNILNQGDNEVDLQIKIIKEIDNNIQEISEMLCKRKNVPISLNMYDRILNIMDKKYNDIKKLNSLILQKQESVTKILQRLKQIEKALKSQQDIQKIKQDKIQLELVYKNVEKYFTKLNFHFNNTDLQKQIEELEKTKKIYEDQNQELNQTITILEQELQNEKAQAQNLITQLNQELQNEKDKTSDVRKKQKEEEENEKAKTLQQITKIKQELQNEKDQSKKQQEQLENEKVQALSQFYKLQQELKKVKEQSEQQSEKLENEKIQALSQIKQLQQELQKVKDQTKKQQEELENYKAQPFNQILQLQLQQELQIVKDQAKKYKQELENEKAQALSQFKQFQQELQKVKEQSKQQSEKLENEKIQALNQIKQLQQELQNEKNKLLFQEQKLQQKLHRKDYAQNFLKEQNQQFNNHQLHKINEQMLKQLNQVNIQLKQNDNLLSINEEQQLSWIDSQLPEIQRYQNLSSNGFAIIQLFINSIFNDSQSYFGLCDQNIDNSEIQNINFQLNYLPKGVYFFISMQGNLLIQDPNIKKKLKIGGFENGNHALFTFDSPNNRLTILKNNTYSEQFKFRLPKNNKTVIPFILQAYGEDLTLIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SQ36</accession>
    <name>A0A8S1SQ36_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Right handed beta helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0100180</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SQ36"/>
    <dbReference type="OrthoDB" id="427974at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000104">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000132">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039448">
        <property type="entry name" value="Beta_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051550">
        <property type="entry name" value="SCF-Subunits/Alg-Epimerases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22990">
        <property type="entry name" value="F-BOX ONLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22990:SF15">
        <property type="entry name" value="F-BOX ONLY PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13229">
        <property type="entry name" value="Beta_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Right handed beta helix" evidence="2">
        <location>
            <begin position="245"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8141536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="623" mass="69289" checksum="267EB2A8D655BE31" modified="2022-10-12" version="1">MNNQDNAIKGVLKQQLIVDSESGPYKTIQEAIDKAEPNTVIKIAPGLYSSNIVINKPGLRLEPKEKVGDIIIVVSSKPTILVDLRKDERCTLIGLKMSHSGTSEEVEELEKLIEGQEMAKHLFGSHEDGGGGLEQNPDEEFVSKVPIEPSMNCVVLLNGGKLFMEDCLIALNFIVKSFKGILPGIAVNQGAEALFIRCEIKGTSSKNQDAKTIGILVKLGDLIIKDSKVHNHTYGGILIQQAVTNKNVRVINSKIIQNKKVGIHVVGSDAIPQIEQCRIENNEGPGIKVGIGNRAKIFGNEIKTNVIGIEVLSADPQIFNNKIDKNFTDGILTKVFEQLRCDGKIKSNQTISGNKENGIHCTGQNNYTRIDSNTFIGYNKKAGIKADTEARISIHKNKISKNLGQGVLLVETASAVIEKNEITDNIKANIALGGANSVDTFIVENKILGGRCEGIFLIECGKCWIFRNQIAENNDGIVCITAVPIISGNVIQKNKIDLKLVRIIQMIMMGQDYLQEIKVMEKLQTMLQSPMRQSQLLKGETLLQNQQYKKIKQLEILEFLRIMIVQYNERIIILFKLLFAIQKGRLYFLKKPHFNLYGAKTQFFNSFSKRNKLTDIELLIQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SQE2</accession>
    <name>A0A8S1SQE2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0100225</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SQE2"/>
    <dbReference type="OrthoDB" id="7869584at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="12"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="328"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8141626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="347" mass="40747" checksum="0B0D74F535FBF1E1" modified="2022-10-12" version="1">MQNTIKVNNKEYTILYQIGQGATGKVFKAQYQNELYAIKKQEYFGEGELEFYDQLIQQKFKRCKNLIQIYDFEQIGRFSYIVMELGDSCLYNELIQNKIDQKNIRFIIKQIGSGIKELHEMGLAHRDLKPENILIKTLFSNDKNESQQVFKICDFGVVKNISNLKTKAVGTPYYLAPELLTNNNDNYSTKCDIWSFGILIYEILTRQLMFQGKTFQEISNAILSATDDDISDKINKLKIEVDYKELLKNMLKVDSQKRYNIDRVLEALKPKSRSTSRQRQNNFVMNPPPQISIRSQFKNRDNQLSQSPFKQIQNFNIIQQQYTIQKPQQYPNKSQQNNQSNVKQLEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SSP4</accession>
    <name>A0A8S1SSP4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0130301</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SSP4"/>
    <dbReference type="OrthoDB" id="285200at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8144591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="238" mass="27894" checksum="8D6A141D5BE39005" modified="2022-10-12" version="1">MQNILSQQQQFFDLLDNQVDAYKTLLDQLSSSQDKFNNLLQDYLLKFQTINNKLVQGRQDFSCSQIQIKRNDELYTHSQLHIQFNLRSLLEKQYKYKLILADAIETPLYRDRIFQLRVELKNKSGDLVKNPNKIELVVAIYSQEQYPKEIKINNKGEQILKGHLCVPLIKGTAFFTRVQIREVSSHYQNGSIILAILPQFKSNDEPINSRDIQPLIVENIKVKAKKFSERHIPVYVNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ST97</accession>
    <name>A0A8S1ST97_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0100268</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ST97"/>
    <dbReference type="OrthoDB" id="296401at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008733">
        <property type="entry name" value="PEX11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652:SF50">
        <property type="entry name" value="PEROXIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05648">
        <property type="entry name" value="PEX11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="1" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00046271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8141712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="260" mass="31081" checksum="6192C7E0E8C91583" modified="2022-10-12" version="1">MLQNFIQFQSSIGNYYFIILKENRDKLLKFLQNWQLLRSYMLIGYNNEESETKFYIYQQLYICRRLFRLGKSLQTILNILEKKQLQNSKKAYNQIPHTVNEMSIYSVYIFYFFFHLIENIMVIHQMGFFGKRFNHKLLKIVSHTFWTLGLLTQLIYYFNRLRSAFRREQELKSQIQNGMTNGEFLEQLKSLSKERYQYGLLILRIIGDLACAMQKAQIPEKILHTRFNRGLVACGGILSSTIQIYLQAAKINKKQNFVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1STU3</accession>
    <name>A0A8S1STU3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0110346</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1STU3"/>
    <dbReference type="OrthoDB" id="298586at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="11"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="125"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8142806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="310" mass="36932" checksum="9CB7E97CE9D944CB" modified="2022-10-12" version="1">MSKINQRANSIDEFRMINKRTQKENQNQNNKTLDEKLLLIEKQLAQNESYNDQYLLTNSIEANAVPMKTQNQSISFNLQSIENDRFNSKKTSIMDECQKKSYDSVRDNASKYQSNNNDSALVMLLNEFERRFEQIEERLQLSEKTNRQYQQNNKSSADYFNGIQEKLTLLLQRIGKFELKLKQLDEAFQSHKNLTDKRIIEYNQHVDQSLKSFLDVSIQIQKKIERITSKDNLTEVEVLKQKTDNLTYNLQRTHELLNQQMSDFSQLQQSVNQVIREDYFNNLFEEIETIKDQYSQTMQYLKELQEFLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SVL7</accession>
    <name>A0A8S1SVL7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PB1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0110310</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SVL7"/>
    <dbReference type="OrthoDB" id="310424at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05992">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PB1" evidence="2">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="116"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="204"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8142734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="362" mass="43155" checksum="6AA872092A584A9B" modified="2022-10-12" version="1">MNIIAKYKEFEKTLENIVTYKDLRKSIRQSFKFILKNNFTIYYLDEDNDKITISNQEDLDVIQNTENFEIIVEEIDDASELTDDSFQKLDKSVKETVNYFRKLSNGHANNDSNSPKIILQQEMEQAKEIIETTNNTQDILDKKRQRVIQEITTTIDHLRSEILKIENDDTEKRLNEKLEKLQKPQMNPSSLSKELSELPIIQQIEQAENNIINLNKVEREELKRNYNNLVKQVNNIVLSRQKNQATQIAQTKEVIKTEQKLKLQITKTNKKNQRLLKQYNGLLSQYQKKLKEAENKKYNFEKIEYMNKEQLENFLDKQMKEELKAKQEKIEKLKQKEEIINIENQKNEQEKLNECDQFEDID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SWH4</accession>
    <name>A0A8S1SWH4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0110334</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SWH4"/>
    <dbReference type="OrthoDB" id="297738at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8142782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="217" mass="25472" checksum="B1AA1631833ADDBF" modified="2022-10-12" version="1">MINQINHNSSEQYKNELTSTKTNPYQTKNIQDASVQVDFSNSQLMKIIDEIDKIHQQEKKALLEQINYLEKKLQQTKAKQLIQCINEVKNIYLNQIEINKPTRVALDSLEQLINEKQHYEVSDLKYRLHQAEKVLKLPMAEFKTYPVEKLVNSIKKCQQFESENTNLANILEELNQADDHWNQIQKDLYDESSTLVNSLQTTTQNIVYELSLLDCNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T055</accession>
    <name>A0A8S1T055_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nuclear speckle splicing regulatory protein 1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0130445</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NSRP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T055"/>
    <dbReference type="OrthoDB" id="446635at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018612">
        <property type="entry name" value="NSRP1_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09745">
        <property type="entry name" value="NSRP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nuclear speckle splicing regulatory protein 1 N-terminal" evidence="4">
        <location>
            <begin position="29"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="190"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="199"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="225"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8144876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="268" mass="31912" checksum="4979ACEA4C638E2A" modified="2022-10-12" version="1">MFNKHQVYLGNKDEVNKEIQKLQQSSKAQQLVSSNLLEDPSLYDYDGFVSKKNKSEKAIVIQKQSEDKKQKQPQFLNSILEQSKRREFEREATKSRMNAKEVVKSGKEVYISESFKKQHEQIKRFEQIEQIQEKINDESRKRNNLGIYKSLYDNETKKAENSNNQEAQVDSYKIIEKIIIEKEKLENNSQITTNNQKNQKQELEELQENKEAINNQLKKTENSKSRSRSRSRSRSKSIKEQKQQENQALTKEDKLKQYKERYLQRKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-02-22" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T1S4</accession>
    <name>A0A8S1T1S4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0140227</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035740369" evidence="2">
        <location>
            <begin position="21"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="53"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8145407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="132" mass="15142" checksum="3E593EF776B586F6" modified="2022-10-12" version="1">MKTLFLIALIAIAFTARVQERSLSKITTDLKKSKYGSALLHLVELHSMAGGPVQELIDAIEELINDLEEELEELEFNFQQRTNEHNSMVIGYQQDIQDAVIDVNNTQDTLDNLLFPRXKTICQFYLQQFSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T9Z6</accession>
    <name>A0A8S1T9Z6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPENT_87.1.T0170292</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Plays a fundamental role in microtubule organizing center structure and function. Component of the infraciliary lattice (ICL) and the ciliary basal bodies.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the centrin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8148086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="312544at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030030">
        <property type="term" value="P:cell projection organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000178">
        <property type="entry name" value="Calmodulin-2 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026201">
        <property type="entry name" value="Cep290"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18879">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 290 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18879:SF20">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 290 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="9 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="673"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="709"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="766"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="798"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="862"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="898"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1876"/>
            <end position="1904"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="6"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="139"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="389"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="584"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="980"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1094"/>
            <end position="1238"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1387"/>
            <end position="1421"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1460"/>
            <end position="1636"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1662"/>
            <end position="1762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1882"/>
            <end position="1896"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8148086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1904" mass="225651" checksum="BA5088A069599A46" modified="2022-10-12" version="1">MSESENFKLRREIIQLKKQIEQLREDGRQKDQRIAFLEAEQEFHQGRYEEQKAKYNDCYTRMRELEKMVNRDDAYTKETLKNQLEKAKDDLDREKAAVQQYIDQLEKVKLENGNLMWQLTQLSLKYKDENIVKDEFHYRDEVQDNREKLKNLVEKTNALNQAIERLSAENRVLRKMAGVPDDYQFELDDVIQEGQLQVEKYRAQVIELEREVEELEEERARLRRKLREQVAGGEVNLEEFDSQSNNQLRKENQELRSKVKMLEEENAHIRQNQQDYGGMGMGTQQGFMKITNDQMQEMMQKQQQELKEQLQEIVKTMKGQNFQETTQIKQSIIQQKQVVDTTGLDKLSVPPNPIPGAFGNYNDIKEGYSQRFNVRFPVDMSSAYGDSGMDQMQLKYELASLQLQNIETLRLLQQKEAEYTNLLQEVVTIKEDIKGKCLLVQDQLFVKYIEERDKFKAMYGEIQEKYKAQLDLNVEAKIKLAHYEDFMRDREKPKKELEDKLGEMAKRTAILDVNLIKLSRKYDALTEEYKQMKTSYTIVESESMAKEKELLEKIYNLISWKKSAEEHMRVMIKELKNSVPADEFNNLRQKLEYTQDKLANAQLKEAEFQKRIATLESHEREAFERGERIRQLEEELVEIQLEEEVIKKRLQSFDPAFDKYVKIYQHIVEFIKIKNISVIEIFKLFDENGDNKISRNEFTTAIGNIGVPVTNEDMEIVFMFVDLDGTGQIEYQEFIRKLKRSGINLRKPEDQLLYQLYNAIKESGYDLRSAFQAFDTNCDNIISKADMKEALIQMNIKHDQKAIDYIFRMADTSGDNQINYEEFYVLFDEIVKSELINQGKSIQLTLDIKYQIMCKLDQSIKDQRLTLLDVFNMIDRDGDQIITLIEFQALFRELEGGISPDLVRQLFDQMDLDRNGQISYNEMLNYLRTAKTEEEKYQKLQFIQQRTEQLKSLQETDLTLQKAKLENKGQTLEDRLKMKISILEMREKNAQQKCEVLLNQLTGTELKLKDTTKTLFELQNQMERSNDTFYRDREEKLMLLERLKGALSREESEKLTKQIEQQRLTISDQNSAITTYRQLYDTAVMQTKSMKLTIEKIKNDSDTMQQTIKELQAISDQNMMIGKLYHHLMVARWHEAQTNQKYEKVLDELRKLKLQSESYDTTILKQSQEIIQQNTIFSEQIRSYEQQITELKLKILPTVTLQRVEEQAKRVKELSDMKSEVERQNRELRDRFYELQLKADYFDNYKEKLDNLERTLRQNNPDELSQQIITVSQKLSDEKIELLRVRREMQVSAEKQDYYQRLNKQYTDTIKKLEYELAHADLDLRKREEDWRKRFYEQRKTIFAQLGLIEKDDMKEFSQKNTDASKMLIDAIQKKQKEQMEQQNQPTEQQQIEVSQLEKRIQELEAQVKAKNIEIEGYIERQKIVAGGSIVFQQQQLEDYTKTLLAQEAEKVSVAGQQMLKAMQEIIKDKDRELERKEQQIEAMRQETLLHKKKDISEIQRLTDLLHKRDQAQQEASKLGNNNNYNNFSYLQQNQMSELQPVLIEKYQQQLSEKDRKIKDLELSFEAEKNALKKVKEELIKTIAIKEQLEIDLENEKKLNESMQYVQKIEKLQNLVNEKNTQLASLKKTVVALKEEQFKIAGESINKEQVYKTKEAKLNNNTTELETRLTKMVNDMKLLKEEIKDKQKKLEQLQEKEIQNRAQMTDLQKKNSELKAIIQAGGGKLEEQDQTVSNIQAKLQQFNQMSMEKVQLEREIQFLKQQGGTRELFDKDATLKPPTGLYFKTFPDLLDTLKQYLITKPKFDLLIEFKKLDKQFTQVLPMAKFFEVLSNAGVKLKTRDQKLVIDTIKTKDENADYYTKFYYVYKGWSDNVVQTDQDDKQQQQQQQQQIQQQQSQKFSLKNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TA48</accession>
    <name>A0A8S1TA48_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0190030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TA48"/>
    <dbReference type="OrthoDB" id="306239at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000793">
        <property type="term" value="C:condensed chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43941">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43941:SF1">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="85"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="286"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="668"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="962"/>
            <end position="1027"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8149280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1036" mass="121521" checksum="3A321BF39AD603D3" modified="2022-10-12" version="1">MSLPQGIPMNAVYKQVVMEHKEDDRVEQLEAQIYNMEKDHSMWRKKYFELESLYDKCRYAAEKSVQLEDRINLLRQDNSQISGLLREKATECEKMRVRLTQLENENFELNTIRLQVTKFNDLLSQKTQDIEQLRKKNSEIYARVIELEGNELVIRDYKAQVNSLTKDLDVWKKLYEDQQQERVKLLEIIRQFELNNPLSGSVKSSQQQNSEVIERRVVTTSETTNNTLQLQSSLLQNQATISDLEDRLGFQEKQYDKLKQELEGRIKINNELKLKITQQECELKNINYLKLDNQKLKDQLDQTKRELDQFRKQYYTTLSKLQIAEQQLNELYNLENRLQLLQQERDTLNKSLKQKNEDIGQLQDKVSSLLSQLEQLRLNDQARNAAENEVIQLKSELNKLQSNLKQKEQTITDLHGKINDLQKQLTNAQSDFKRLQAKDYSGLQDQAKLVNELQQKLLQLSQDNSKFQSQAFNLESKNNSQVQDIQRLNSLLQTKTQELQDANKKINDLENDKTIPDLKFQNSQLIAELREQETENQGLQEKLQILENQIIQLNTKLTTAEAQANCNLNEKNKVDQLLSSYLLQLSQLEKEVDELRVIAQQVSQQSAINKQQEQHIQALQKQLKEYSSQFNEVSKENEKLYVDNSQLSTRISQLSELPNKIQILDEGIQKLQQDYNNELQANETLNKQLNDALKVKNDLENKIAMLSTEVERFQYKLNSRQNEAEQLKKQIVELETQIAQLKQLESDNQVLADKIAHLEQQLINEANKNLEHLLRVSQDETKSYILDNNNLKQQLDQKEQENKQKDESYRQLEQKYQQLQSSNKNLEAQNTQIAELQNVIAGQESDIQKFQDLIEELRDKNNDLVAESQKLQIELTQQQVQISDLKNQIEKLNTQVNNNQNYDQLIEELKRKIEKLQEEKYQLENKVALLSSEIERMRVKYDAQGNKLNTSEEQRQGVTKELEQVHKLFEESQDEIDKLQEQLSKQKELEQKEQALSLTLVNAFNEIDALRSQLQKVSNEANEQKKANIVLATQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TAQ7</accession>
    <name>A0A8S1TAQ7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0190155</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="285774at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="10"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8149530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="279" mass="33366" checksum="6C86066097773EEE" modified="2022-10-12" version="1">MKWILKSSXAFKKEFQDKQKEIKQSNQEERNEIITTLEQLNQKIKVSEDVGNDIEEAFKKIQSYVNHKEYEMKHNKEGEIVNKKIYLLNLMKDNFVKMIKNYFSNAFTIEFCRLFDMQMETLNSKEVQDVNGFLDYVDSKWKQDYKNLQTSIEKFVQKLHEGLKYIIQLPLQEEKPLKQQIVIPQFTSPSRNPIQYQQQPQFVPLTPYNQVPPQYQFHQQIPLTGQRVYQNQNQFNSAPVPQADVENDYFAQSQIIYEEPQMRNNTRPDFVSQSLQQYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TAU0</accession>
    <name>A0A8S1TAU0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dynein light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0180051</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TAU0"/>
    <dbReference type="OrthoDB" id="282425at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21450">
        <property type="entry name" value="DLC-like_DYNLL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001372">
        <property type="entry name" value="Dynein_light_chain_typ-1/2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01221">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01375">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8148409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="95" mass="10963" checksum="548CF4A9B0829B4F" modified="2022-10-12" version="1">MDKSVKINEKGTGITDQIQNEIVTIVKNAIQVHKQFLGEVCNQIANVLCQKYGGWWCCFVTDKDANYGSRLFHFDHMYLDATIEQKRFTIFKSAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TDF6</accession>
    <name>A0A8S1TDF6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="14">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PPENT_87.1.T0180333</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21 14">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 14">Membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="14"/>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TDF6"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000118">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000084">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF150">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 13 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="55"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="285"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="327"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="879"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="911"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="979"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1013"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1042"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1083"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="17">
        <location>
            <begin position="17"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="16">
        <location>
            <begin position="113"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="18">
        <location>
            <begin position="847"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1234"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1248"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="11">
        <location>
            <position position="398"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="398"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="398"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="399"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="400"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="400"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="530"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="571"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="593"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="626"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="705"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="706"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="707"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="796"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="802"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="821"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="824"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="825"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="825"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAD8148969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1258" mass="145289" checksum="E00407A460147C16" modified="2022-10-12" version="1">MFKSDRQKSDKERIFTINASGKANNEVFPTNFIKTSKYNIVTFLPLSLLGQFRRYANIYFLFIAIIQSFPIISPLNPISAIAPLVFVLGLSMIREAMEDISRHKSDNEVNAMECTKIINNKQVKTTWADVKVGDLLYISENEMFPADIIVLSSQFESGVCYIETSSLDGEKNLKPKSAIKETQTIFGFNETQSQNQMSQNNNQQFKAQGNPPSPALGDFEGSIHFPNGTKKVLNGKQLLLRGAFLRNTKFIVGVIVYTGEDTKIMRNAEPSRIKQSTIESTMNKLILGILGVQIIACALSAILSSWWLHKSFSKHIYIIITEQNYTLLSAIAFFSFFLLYNTMIPISLVVSMEFVKVFQCYFINKDQDMYSQKRDKYAKAQTSTINEELGQVEYVFSDKTGTLTCNQMEFKYCIIGDILYGQNEKINNLPSPKQNNHNVQEDTSQFKHSVFNFQDNTLTGIIQDKPEYQNQSEQPRKLIIQSNDKNATLEIKTQKELVHEYLMLLSSAHECIAQKGKNSQINYQGPSPDEITLVDAAMNLGYRFEGQSANEQEFTIRGKQKKVELLSQFEFDSNRKRMSVIIKDNGIYKLYIKGADSIIKARLKPDQPFLEFIQNKLSEFSTIGLRTLLMAMKILSEQEYLAFERQKDALASSDKREQEKEELVNNLEKDLYLLGATAVEDKLQDDVPETIADLLKANIKVWMLTGDKLETAENIAKSCRLIQNDFMVMKYSETDLNKLRVQLSENKLTYQACIKEKKKKSILVEGESLVILTNNQQLKREFTKMAMGCDSVVCCRVTPKQKAEVVHLVKDLNKITLAIGDGANDVNMIQEAHIGIGLYGNEGMRAVQSSDYALGEFRFLWKLLLIHGNWSYIRISEMILYFFYKNMIFTVPQFLYSFYSAYSAQTYFDDWYITFYNLFFTSLPLIARATLDRSVYYKMNVRNEEYSDIYQKSTQYLKGKFPLLYSVGQKQTIFTLPNFIFWWGQGFIHGLLVYFISYACFDTELVNLNGQNAGFATLSITAYTAIIFIVDIKIAIYTKFWTFINIITLLLFSIGIYIAYFFISHYFKGTYSEFTPIYLIQSPNFYLIIALLNAIIFIFDLVINAIIHEFYSTETDKIIKWRREFKQLAREGNVLQKIEMLQQGKRENGQRDWGDSFFEQILEERLKSDRDQNIVEEQRAILDSKFQPYASPVNLNNSDQTNRQCQQRIELQQIQLPLQNNNIKTINYQMSQKQQQNFKNVNEDSRINHPQQQRIYVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TJC6</accession>
    <name>A0A8S1TJC6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0210161</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TJC6"/>
    <dbReference type="OrthoDB" id="294131at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8151079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="162" mass="18926" checksum="9D8BABDF5C914B4B" modified="2022-10-12" version="1">MQLSYDELCQNKLLAFTIFSKVKAPSSLIEQSDTAEFKLALQEHRSRVSTTLYCSVLSSFTFCLILKMRNPIYALTARSSFFGVLKKYYFVPVLLSQPFQTQNRQLYDEKVDKVLKKYDFTTPEFQQAFKQIEADGGLKKFMKKYLTEKDEEPQQPVQQTQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TM19</accession>
    <name>A0A8S1TM19_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPENT_87.1.T0230254</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TM19"/>
    <dbReference type="OrthoDB" id="288306at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021319">
        <property type="entry name" value="DUF2921"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050731">
        <property type="entry name" value="HRD1_E3_ubiq-ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763">
        <property type="entry name" value="RING ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763:SF162">
        <property type="entry name" value="TRANSMEMBRANE E3 UBIQUITIN-PROTEIN LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11145">
        <property type="entry name" value="DUF2921"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="254"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="279"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="322"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="374"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="404"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="469"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8152814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="539" mass="63994" checksum="525F98B44C2D67CB" modified="2022-10-12" version="1">MLPHNQVEQGNDVRTRRIKFLIFLSISAMIMYSVTYTEDSEVHHNSLNKCLNVSGDQNMLNYIQNSTNKPSTECYLYQFLSFFAERHFKGQLNRTYTHFHVYQYSQLKPDFNISVLFSNSPYLDDPQQQFQFYLNVVEYAKTFNTPLILKVQNNDTTGCQNELIMELKNITNNMNISFAEQLIMDVRVETQCQNFHQMFLGSVKDNTEEVESQVITYSFLNSVVCLCCMIYGLRILRALIEGLDNPEEYSMFSIGFVMVQDLYMCFLNFFQAMQSEYLFQYFITPSFLQFLLFSIFEMRILMIIWKSRVRNDQSFRNQMIKFYLILYFCMFGSMYLIYEYVLSSWLLFFLSLYLVPQITHAAQRGQIVRFNFKLICGFLLPRLLYYAYFRFYWNNLFSLKPMPISLICIFVSIGIQIGVHILQNEWGPQFFVPKICFPQKYNYYYKIPTSFDLEEGPSSEYAHLFHEECSICMGALHQVPAIRLTNQEERNYMIKEAMKKQKNHYMKTPCDHKFHVLCLVKWMSIKLSCPSCRQSLSPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TR44</accession>
    <name>A0A8S1TR44_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Proliferating cell nuclear antigen</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0260370</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PCNA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8155215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TR44"/>
    <dbReference type="OrthoDB" id="534348at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043626">
        <property type="term" value="C:PCNA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030337">
        <property type="term" value="F:DNA polymerase processivity factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019985">
        <property type="term" value="P:translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00577">
        <property type="entry name" value="PCNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.150.10:FF:000006">
        <property type="entry name" value="Proliferating cell nuclear antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00317">
        <property type="entry name" value="DNApol_clamp_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000730">
        <property type="entry name" value="Pr_cel_nuc_antig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022649">
        <property type="entry name" value="Pr_cel_nuc_antig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022659">
        <property type="entry name" value="Pr_cel_nuc_antig_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022648">
        <property type="entry name" value="Pr_cel_nuc_antig_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00590">
        <property type="entry name" value="pcna"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352:SF0">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02747">
        <property type="entry name" value="PCNA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00705">
        <property type="entry name" value="PCNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01251">
        <property type="entry name" value="PCNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA N-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA C-terminal" evidence="7">
        <location>
            <begin position="126"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8155215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="259" mass="29137" checksum="73CCC956F48C15B8" modified="2022-10-12" version="1">MFEAKFNDGVQFKKIVEAVKELVKNVNLEANGTGISLQAMDTSHVALVALQLNEKGFKKYRCEKSLTMGLSIENLQKILKCSGNDDQITLRTQDEEPTTLSFTFESKNRISEFQLNLMSLDQEQLGVPDTEYSSVVRMPSNEFTKICRELGNINEAIGIETSKDGIKFYVKGDIGEGQVSVKAYDTEKIEERVECDVDEPVNLSFAVRYFNLFNKASALSNQVVLSMSQDQPLVIEYVLEEMGSLKLYLAPKINDEESQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TRH2</accession>
    <name>A0A8S1TRH2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ABC transporter ATP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0250211</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8154122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TRH2"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000461">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF1">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B MEMBER 10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="261"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="80"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="387"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8154122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="600" mass="67747" checksum="DE4D770F06772404" modified="2022-10-12" version="1">MQEGKKSKKFFGKDIMNLLKLMVPEKRFCIIGMTVVGLNSALLLYLPQVTTDIAKLQELKYDQENKEKSQQTNLYYHVKWGGVCLLGMFLGGARRFLNTQISNRVGMRMRYAMFQKLMSTRETQQLHSQQLVHKLSNDVSLVSTGLSQDIFVMIRGFITTIGGAAYLSYYAFPLLASASAAAAVMGGSAILLGKYMSQFKVKETQELGCLSEVSQEMLQAHRLIKLSDMEQKENQRYSAQLFRWYEQATQFSKMQALNMGLMEGGGLYALILILFQGCYLVATGHLDPELAKYFIQAVYMASGTRAMIGIYNELVKTAAIYKTILDVHPNLHESELYNQSDLTKNYREYIRQDDILKAKLLNPPQSAEHTKEWQELYEHSSIPPPSIQFKNVQYSYPNHENSTLTFNLQINSGEIVLLQGPSGSGKTTALNLLTKLLTPSSGEILIDGEPLSKKDFKWIQQHVSYVQQDGIIFNATVYENIIYGNHGYDQSLERVIWAAQLARAHDFIENMPNKYQTKLKSDGTTSLSGGQKQRIVLARAILKNPKILILDEATSNIDSQSESDIIQALMQICQDKTVIIVSHKIENYKPLLTKAINLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TST5</accession>
    <name>A0A8S1TST5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0260309</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8155093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TST5"/>
    <dbReference type="OrthoDB" id="289272at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="376"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="10">
        <location>
            <begin position="635"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="230"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="753"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="231"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="243"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="766"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="405"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8155093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="870" mass="101370" checksum="76C05ED93C2BFBA1" modified="2022-10-12" version="1">MKNYASHPLLSSGRGSLQDTPYYMELKPKKNNSKLAKTERTTAKNTKQDLLEYFKKERNTLAQVSNSIKTQLQQNRSIDNLISQKSERLKTLNAQYQQFPLSLDQRIHTDEGNNIKRQIVKGKNLSLATIQMESKQQKIAIKQGKVNQTPLNNHKVPKSTVDQYFQDLQMKSAKQYKNNHYLDYQQHFNFRPNKNSLLQESQTLKQSIEYILTKNKEDAKKAIKRIDSTEPRSINQSTQQKTDSIKKMNRADDSNHYDSKKQLKIILFYKNQKYNYLFEYQNKTTDNLYNFLIQQIASIEKSFVKQGGGTGSTEEDHIQQEINKVCQFYCVTKNVPYDYYLSLPNLPLNVFQGITLQLQPLIAQQPQGKRVTLKDFTLVKCIGVGGFSRVYLVRKRDNGKFYALKLIDKKFIMDNQKEVIVQNERDIMVKMDNQFITPLHFAFETKYYIAFVLDYCAGGELFFHLRKLKRLSEENAKYYFVEICIGMAYLHSQNIIYRDIKPENILLDLHGHLLLSDFGLSKPNMAPEDFAYSFCGSPEYMAPEMLLKTGHNYLVDCYCLGALLYELVTGLPPFYSHNTQEIYNSILTEQVQFPPYVQISDELKDLIYQLLEKEPSQRIGQSQGVIEILTHPWFADADFEAIVNKKIKPPYKPQPLQYNFDEEEFNKGDAEFRKQYNINLQKEYTNENFSNLILQNFYFSREDLPQKPEHKSKRSNAINLSQLHLEGVPEIQNSPERIGVQAFSTDLRRVQKYQGKSEVQDGLKRNNQNNNKSNSLISSSKIGHAYSKTMQQQNSHPDLKTLKFMIDSSKIQLSTERNATLPENQSSKFQNNRIKTEQITQMLYPKTTTNQQFNKQSNLQKLFGSDKRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TTN1</accession>
    <name>A0A8S1TTN1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0260215</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8154907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TTN1"/>
    <dbReference type="OrthoDB" id="304955at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8154907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1107" mass="130613" checksum="D9C72B6AC826D3BC" modified="2022-10-12" version="1">MENYNFSALSISIQTDTQSSQSDQQFKFSSPLKSEKNNFLDLDNQKENNDSANQSQVEVSIQKDCQEKVAQTSEQKSNSICGSQLKQTKASQNKKRLVKRCQTTILIGQSIHKHNAQKADKYFMKKNNQIIETSFEKLKVKRSNFSGIFLTNLRQQQKPIQVRYFRSTITKVLVIEQDMLAAEFIVLALTQFQNDSKLDQSLYEFPNYSLAYQIEGQDDQQDLDEFSYRRPLQRYKSFGSLEDKMKSRRESLDEINYDQKPVSQAEISLLADDIDDKIIQIDLESPMRYYKSVDIVPILDQKQLDIDLQMVKILHQQLPNHILILIEEPISQGRFHIKIRPNQLVEELFIELSRKANKKYRSTDYNLILKYPCIHMSKEQINLKMPISKLPIHYLVLTSKFQNETIRSLRQCNDSFTVNINKDTIFSKSANKSYRFLQEEGKMSESVDKVRNQYSYQEYQLLKIDKQFKNNVILGIDYFDLYYTYTQERQRQFTLGKLCKSISQILFEEPLIQKDYRRLPLNKIQKVELTKDFNLIIKIASFWDRFKLREIKNRFAIEIYNECIQQIRIFSGFIYKIQITIMNLFNIDNSLATPYSIQSNTMSVFIDNDWLTKQLENVQQIDYQNYNLPSNTDISEFVLQQSVGESPFIQQHKRDPVDFSLIKLEKLEEIQQINYTPKKQYSSTTRKQNYKKSKTTTTGFAIDENDTNNNDSETLRKIKSSQVQKQSEFTKQLNIYQQNFSIQILVKYFGTQYQQIISVDESIRAAEFIVLALKRFASDPKSDKTKFEYQNFTLAYKLIGQDISPTKYTYNNDDNFNNESVILEDESLERPEVDLESQLFYKIVDLLPATTNFGLDFQTIKQTFISNPESIILLIEDPQLLIFYHIKVDKKGILADCLVELNRKVSRKYLKNDYYLSLKFPCVNYDTGELSEKFPINQLPIHWLIIIQRVKNIILESSEEVSMANIGRTFNNTIHENMRGSNSYGFLSMNFMQLTTVEKLPLLYSIDYFDLYYTFNQKQTTKFRITKLVSNIIRSLFVQETEKKKYKRIPINTITDLKQQQEKYFEIYYELQDGYQKTIKFFSKNDDKTVFREAFTKLEYLVSIQQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TWU6</accession>
    <name>A0A8S1TWU6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0280267</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8156333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TWU6"/>
    <dbReference type="OrthoDB" id="10249433at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052920">
        <property type="entry name" value="DNA-binding_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43358">
        <property type="entry name" value="ALPHA/BETA-HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43358:SF4">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="69"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8156333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="336" mass="38705" checksum="1272735390C33CF7" modified="2022-10-12" version="1">MKQEEHKWNEKLWKPIIRPQRYSYQLSGLGSESFMVQGIIVKRFDFTIKNNRNLILQCSLFEPIKLMDKPHPCIIYLHGNSGSRIESHSIIDYVIPSYISVCGIDLSGSGQSQGEYISLGYWESQDVQCLYNYLREEKVFINITSIGLWGRSMGSATAILAARENPNIRVLVCDSPFSNFYMLCQDLAKSRYHIPKCFFNCCWCFIKSKIQKEANFNLDDLNLLSIVQTLQNDLLIIFLQAKQDELIQNKHASLLMENFRGKKTLLSFDGTHNSIRPKEVMEQSVELIKKEFGFLSWNQTSFQKEIKLSSRNSSVNAPLLRASKTSIVPQTEDTEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U004</accession>
    <name>A0A8S1U004_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0300054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000030">
        <property type="protein sequence ID" value="CAD8157243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U004"/>
    <dbReference type="OrthoDB" id="295353at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8157243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="131" mass="15354" checksum="D80B1070094EE975" modified="2022-10-12" version="1">MNKLYIESIKTSAELIPIDIYFLLKIGEHCQFSNILSLQQQSQTCNMCIQYQAREKQPISIECWAYDQHLVPKAFFGKEKLTNTTRKITLDGMEIQIRQSQQERSRTPEIAMKRDASPFGKRDLTPKREKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U9A3</accession>
    <name>A0A8S1U9A3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0360320</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U9A3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8161705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="198" mass="23076" checksum="CACD10458CB15170" modified="2022-10-12" version="1">MKVQMIIHKINQLNTQITDGYFSVCQYKIKQHQYSCKYCSHSFINLLDTYNNISRKKQQLRYMYTQFQSQKLKEHPNLVLLNSQNIIHQVMKQIGGTSNITYDFCTHKFFLQAANSKISAICSTISAGGGFLECQSSSNCTKYDIVRIRLGDSSNNYNNILIKSLPSYRKALLSWIFYTYQYLYATCNDPWTLILILH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UF93</accession>
    <name>A0A8S1UF93_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PSI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0360114</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="288436at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002895">
        <property type="entry name" value="Paramecium_SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01508">
        <property type="entry name" value="Paramecium_SA"/>
        <property type="match status" value="26"/>
    </dbReference>
    <dbReference type="SMART" id="SM00639">
        <property type="entry name" value="PSA"/>
        <property type="match status" value="42"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035740630" description="PSI domain-containing protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="4029"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="131"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="320"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="3055"/>
            <end position="3107"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="3121"/>
            <end position="3168"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="3188"/>
            <end position="3235"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="3322"/>
            <end position="3376"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="3">
        <location>
            <begin position="3672"/>
            <end position="3717"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8161326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="4029" mass="444659" checksum="A7424C2E30B8E822" modified="2022-10-12" version="1">MKLVILFLFMCIYVVQSETINLSLSSPCQCTQLFEYDCYRNQNCVWNKLQLRCEIKSTTEESNTNTKTKNNTYCTKFGSDKCWETEGCAYILNECVQFTSCSSYPFSVQELCQMISSKCITDGEKCIEIGDCYQYQTSISCVKNSKGKYCFWDQYCMDATDCNKLPVQFKTDAECREQLSYCTVNPRGGCQVSGYNCEDQEFEMQCYYNQKLEQCAWVDGKCMDRSCETAPKSIRTDIACQEYFQSCTLIAEGGCRQRTQCNDVTIKESCTYNANGQQCFWNEGKCYDKKCENAPQNINCSEFLENCVPKSLGGCMSISECSKYDMKESCKLDTQNNKCFWTGSYCILYTCQSAPLDYVTHEQCAAYKEDCTVNNTTFKGCSERTCDNAPTTSTTFLQTNYDCNKYKEGCITKLGGGCKQLNECSDIDVEIACVVDKYGRNCFFYNNQCALRTCENAPLSFNSHQQCQEFNQECTVARTLIGCVKMTCQVLTSQSTCKVDLNGNRCSWTGLCYSKTCANAPFEYDTDLKCKTYLSSCTIANSGRGCITRPDQCNLLVLEDQCYITSTGQICGWFEGACYDKACFTAPYTSDYDTYEECNNYLNGCTVASEGNGGCVPLDSCTTYTALRSCRFNNLNQLCEWTGTACYDKSCTTAPQDADHDSNEECEAYLTGCIVAPSGQGCVQKPNSCGLMSTNTQCTETSTNSLGGPCVWVGSCVNRTCNNAPISANYDTHQECYTFLSSCTVVPTGLGGCMTQLSTCAQYTTFRSCQLASNGQKCAWQYNYCYNRQCSYAPDTNEFDSDTECNDFLNSCTVVRRISNLGCTSRLSSCQDLTEQQCITDSNDNLCTWDTTLATPNCKTRSCTFMIGYDYTQQNCKNWISGCVVDNNIPHTNCMTQRSYCFQYYNQDNCEYSTYHGYCIWDGSTCGIRNCSTTQNITDFNHTNCNNWLSSCTVGAPTTCIQKPVNCADYVNYNQCYKNYADQPCTWYNDGCIEKTCTNHGYSVTVFNQSNCNNWLSSCSVNPGNTACETTRTCANYTGSTFTHSNCNSWLSDCTTNGTQCVNKTCTNYGSNVTVFNNSTCSTWFSECTNDGSTGCKSARTCTNYGSKVTIFNHTNCTNWLSSCTNTNTACTARTCTNYGQNILFFSHDTCNTWLSTCTVTWDKLGCETKTCINYGQQITTFDNTNCQAWLSQCQANINGTACESSNTCPSGAFTTVANCGDYLESCTYVSGSNCTNKNCSSPSGITTYNHKTCTNFYKLCTSNSASNGCEWRTCYNHKGFISYFTHESCENWLFQCTVNSTNTGCTVKTCTNYGSHVTSFTNADCNKWHTSCNGATSTCQESRTCTSAPTGYQYEHTNCEAYSLSCTKNTATSCMTKTCAKANLVFSTFTHAICSEWLSSCTSDATGSACISRTCTNYKDQLSTISHATCNAWLSSCTSNIAGTACETITCFNHGTQVTVIDNINCNSWKSGCVGGGTTCRSSTNTMCGSYSGTINHANCTTYLSTCTNSGSTKCIVRTCSLASQGGITVFNHQKCSEWYLECTANSDGTGCQYRTCSNHGDYVTTFTSTTCQNWLYGCNVNSTNDGCVSERTCSNYGSYIQEFTHENCSNWLSTCTNNSGNTACIDRTCTNYGSNVTVFTYTNCNAWLDGCTGTGSACEARSCSNSSTGISSYTEANCYKWYSYCKVNDSLNSCISTRTCTNHSGNITTFNHSNCNSWLSDCTVTADGLNCETKTCTNSNITAGNFNFNNCNSWLSGCIANIQANNCETVRTCENAISNLTTFNHSTCSGWLSSCTYNKAGTVTAGTSCIEYVCANAQLTTFNHTNCQNYKNTCTVNSSNNGCTDFICSNAANSLSSFSHQNCNSYKSTCTVNNSYNNCEDMTCSNAYRTLQYSVTYYNYAHSDCQGWLSSCTINSSATGCETKTCSNASYTQTIYSGYSCTNTWMSSCELHTSNSYCVEKTCLTFRGSLSTANCNNYLSGCTYYGNYDNTTSCTSNIRTCANAIIAHNNGYSIANQAACDQWKSGCAFRGSGNYGCEERSCSNYVSANQSNPSTYAQCNSWLSTCTYDSVNQKCVEKNCSNFSQFSPSFANCQAWNSICTVNSTNTGCEKRNCSNYSYVYQVFNDSNCSAWYFNCKVNSSKTACEASCTTTTGITTFNFANCQDWDTMCSVKSDNTNCEKRSCSNPQLASYTDTTCSSWLSTCTNNGTTSCKDRTCYNYSTNLLLYSSSTCESWLFVCTNYKTLGCMPKTCDNYTGTVNDSNCQSYLSYCLANTTKTKCVTRRTCTNHGSSVTTFTHVNCENWNSNCTSNILNTACIEKTCSNINTSAVIQINSYTCTAWKSTCAYINGSCVDKTCSNSGLVGSDVTLTNCSDWLPYCKANNAIWPQYCELKTCQNHSLGTINQANCQNWLSYCRVNSAGTACMTDRTCSNYGINILTFNHINCEAWSSYCTVNTTNNGCMNKTCFNTNLSVFNDQTCGQWLNTCKANSSNTGCEIRTCTNYGNQLTSFTAATCSYWLYQCTNNLSTGCKTTRTCTSYTNLIQTFTLQTCSEYLSQCVPDPTKSSCLERTCSNAQELPSYTHQNCTNWLNSCTVNSSNNGCIDRTCQNANLSFYNYSSCSNWLSSCTVNTTNDGCTAKTCSNSTITQFTQSNCTAWLNTCTNGTNSCINKTCANYTGTINQTNCVGWLSYCYADSASQTQCYSLRNCYNHNLSTFTVSTCSSWSPLCTVNTTNNGCIEKTCYNHNLTKFSHAVCQDWLSTCTVNMDGTNCEIRSCTNYGNNITLFNNANCSGWWKYCKATTLGNACEPYTCWKNNVSVFNHTNCYNYLDQCTVADSTSCGSTRTCTNHGSAVTIFNHANCQYWLTKCTVNAAGTACEEMTCENHGIQVTKFTHANCQNWMFDCTVNATGTDCELKTCSNYGANVVVYTHANCLAWLSKCTNNSTNSACINKTCLNTTGISSWTQANCESWLSVCQLSKPTTCTNNVSNCSSATYNQCVKDTNNDICVWHQNSCKDRACNNFSGTANHTNCENWLSTCTVNTTNDGCVTKPSNCTTGGVTQNQCVISYSNVKCAWNGSACVNRTCTQYSGTFNHTNCNNWLNTCTVNSAQTACQTLSSSCTSYTIQAQCNITSSGVVCFWDNTTCVNRTCEHAPQTTDYNDHTKCINFLNSCTVARFGGCTDKLSTCSSYKQQSQCYQSSSGVKCYWNTLTSLCTDAICTNALSTLTTHEDCQSFLNTCTVNTAGGCIPLVACNLYTTSLSCIISNTGDTCEWQSGTCNIKSCTTAAFDTTRDTHEECQNYLPNCTVIASGIGGCVPLNDCNTYTSERQCKINSSGLLCGWNGSVCANRSCSTAPPTVTYSNDSACQDYMDGCTVVETGFGCESRKSECSDYKVSKQCVKTITNQLCYWNKELPIPICEITTCLNAPSNSLTPALCEQHFNLCYSNMQYCRLEECEDLSYTTDFECKYYNSKCTTNGINCVVRKKCGDVKNSAGCVMDDQFNECEWFNEECVIKTCYTAPEYTTEQECNLYKEGCTTKLFGGCRVKTTCSEANVEQACTTSNIGEKCFWQEGFCRAQRCEDFDGSYDDICDAQKKGCVSDGTKCILPRLCSQILIKDQCIKGVDGPCVWYEFGCTLFLSCSSIQSNLDRVCKSANSLCTTDGLRCVGLEKCSNYRLQEACRVGLDGNCQWIESLQKCLMFVKCSDLPFLTHDECQKASSKCTTDTLNGCINLEQCFNYKQKEQCKISSQPQKLVNNQIIQTGYCAWVNGKCRDQLCEDLSGETHESCQAKLKGCTSDGTNCLTMQPCSQYSNIITCNLALGTDGKCLFSTAGCRTLECTDIKNGTNHFTCQNYNSTCISNGDKCIPQVKCEYYPNQLACTYKGLDGQCAWNGNNCALMKSCKDANIDMIACQRMSEFCNWINYANGTSSCLPHTCQTKGILNQCNYIKDFNNQTISTCFWSNDSCQSVDPRNLQSPECNFNTLNTYRWNPENNTCESCSPQQINNTNTPLANYLQLLSIMLIQAIIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UG05</accession>
    <name>A0A8S1UG05_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DUF676 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0390158</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UG05"/>
    <dbReference type="OrthoDB" id="273452at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001166">
        <property type="entry name" value="ADP-ribosylation factor D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000343">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022122">
        <property type="entry name" value="DUF3657"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044294">
        <property type="entry name" value="Lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482:SF5">
        <property type="entry name" value="DUF676 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482">
        <property type="entry name" value="LIPASE ROG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12394">
        <property type="entry name" value="DUF3657"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF676" evidence="7">
        <location>
            <begin position="543"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="378"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="23"/>
            <end position="30"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="125"/>
            <end position="128"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8163314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="801" mass="94276" checksum="83A7704D2BA052FC" modified="2022-10-12" version="1">MGLLNIIRKIKKSEKELRILVLGLDNAGKTTILKALSNEDINQIAPTHGFNIKNLQHEGFKLNVWDVGGQEKLREYWSNFYENTDALVFVIDSSDQMRLEEGGKELDKLLGEAELKKVPLLVFANKQDLVQALPADEIDNGRLQHVQLKPKKDYKKEWNGLLRQSKINEYQIINIINMKSQIEVLIHFKKFTNIDLFTQGIYQIRVHIPEAQPYLIFKSIRQDPFTTNEVDKNFVFYEENIEDKYFYSQGFLIRYEDEQMSTNIGCLFRQQETQNIEIVIELLFLDKKLLGDIYVDDLQSVALSLRQQMQIISKATLTVSNPLIYNQAYYPVEFDSAHFSLLETQIHTIPIQFQFTKQQLIDEFKTQEQFNEVINKSIYLLLNNREQLIKQLNNLQNEKKLRQLQYQEKQLDFKDDDKENQIIQSLHDLHHDMYILWCELVDAIKENPQKLQDQLEQEFLCQMIQRWQNCVLLNKSEVKQLDQVQQNGRNNHEIAKLKRNQLFSQEIDAIRYIELLQPLTTNPFIFKQTCVQNGFYQKPQNSYVHYVVLVHGYQGTSYDMRYWKSILTIRFKDKIRLICPTCNDGTSNKPIQEQAKLLAEEIENYLSDENINEYRLSFIGHSLGGLIIRAALPQLREYKEFMHTYVSLGSPHCGYASSESVLVDTGLMMIQKWNKCKTLEELSQRDHKDVKNTYIYNLSKAEGLNWFNNVVVMSSFQDHYVPFHSALIQKIENVNDQKVQAYNEIVSNILSKCGKIDRFDINFQITKKKLDKFIGRAAHIEFIDNLTLVKMFVYLYDEYFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UGK6</accession>
    <name>A0A8S1UGK6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">40S ribosomal protein S19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0380298</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UGK6"/>
    <dbReference type="OrthoDB" id="286522at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000118">
        <property type="entry name" value="40S ribosomal protein S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001266">
        <property type="entry name" value="Ribosomal_eS19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01090">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01413">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8162899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="147" mass="16511" checksum="F535932A904B3037" modified="2022-10-12" version="1">MSAPFFTVKDVPAQDFIRAYAEYLKKNNKIKIPEWGSIVKTGLGKEISPIDQDWMYVRAAALARKIYVRGHWGVGNLTHMFGSVNDNGKHESGSGKVIRYLLQQLEAIKVLKKDNKSLLKKGSRIVTKEGQQDLNRIATQVALAARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UJQ8</accession>
    <name>A0A8S1UJQ8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0400173</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8164002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UJQ8"/>
    <dbReference type="OrthoDB" id="7869584at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="14"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="329"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="410"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="271"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="329"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="358"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="373"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="456"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8164002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="727" mass="84535" checksum="231044BF9461F84B" modified="2022-10-12" version="1">MKEGNEIQIEQNTYLIREMLGQGSYGKVYKGQTKLTQQTVAIKIQDNISEYELMMLQKMKGQKFKNLINILNLEQIGRKYYIVMEYCKESLFERLQRKGTIKAEDIRFIMKEIGNGIKEIHDLGYAHRDIKPENILIFQITDNGNTQDLYKICDFGTIKNIDVLKTQVIGTAYYLAPEQVNGSNQYSKKVDIWAFGALIYELLTGQPMFDGSSEQQVWLKINNITQKQIDEKIQNNLKIERKYRTLLLNMLQIDIQKRHDINQVTCDLRGTSQMTHMPTNGQKEKPNNINNEEKHVNKKNYYDIQQLIGIPNPIGFQQPINQQNQQKIFNNQPNQPNLPNQPNLPNPNDIKLQQQQQPRERNTDLEDKIKQPNFPPSFSPNFPPQNQFKQQEQLLNLSRGRDINEEEKTKQCIYRSPQPPPSTQNQPGYSSIRSTMKPMLSRKPEARAQIEIQNKDIGNSQPITPIQFQNQPGLNNVKGNLPQTNFQNQQEQNKFRASNNPQGIIPQTNIPKQVSSQPFNNQEKNQNFLQQQQQQYIIPNQQQTRQSTNLQINQFPQNSIQKQVNSQPFNSQNAIIQQQQSNQIPNMANQNIQQYNPQNNSQPCDQQQQQNNQFNKVQQPFNQQFPQNGNFPNNNTSTQHNYVKQQFKDDNSSFQQNKDHLTQTKQQSLNPMQGNCFNSNNNQARQTKLASQQPNIQIVQQFGLNQQTSQKQNNNAGHLYQNNRPTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UNV4</accession>
    <name>A0A8S1UNV4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0400240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8164136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UNV4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="149"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="274"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8164136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="452" mass="54333" checksum="6887BA1516DC71FE" modified="2022-10-12" version="1">MNYHNPDPYQQPYPQGQYNEKQYRYSGFLTETQDDYTDYQYYDPYQSQLHNRTYLNDVQPLNYTYNEYPPYPVKMGQPQLYFDPNDPSNIPFMPHERYDDGYQEYVLQFIKNLPRSTQNFEEVAFAKYIAECSIIDKKVWKLQRMLKQDQRFNVKLLFDFFDSKKNRTIDYNEFQLGLQKLDIILNSIELTQMFLRYGGNDDNQISSAEFKYMLLGPDDQQQFMQFGAMHYMHHPLKQNIPQFLSVAQREWICEIMNLQLQLERNLNFIREVIGTKDILLALFKQLDIGMKGYITLQDLVVYMNELGGQFEQYDLKGCLDRMSKVHCSRINYNEFKKEFFLDQKQKSDQDQNIDIMSDRKKTRQKDYIQELHQRTKDIQNQQQQIQKLSTDRKSQPLLTTTIQNKQPQPYVIQPPVVQKKKQEQPSQLDDDLDDLLFPGFQKYELQPPDQEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UR97</accession>
    <name>A0A8S1UR97_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UDENN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0460260</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8167161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UR97"/>
    <dbReference type="OrthoDB" id="292665at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032483">
        <property type="term" value="P:regulation of Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051696">
        <property type="entry name" value="DENN_Domain_GEFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296:SF21">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00799">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="2">
        <location>
            <begin position="20"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="585"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8167161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="744" mass="88310" checksum="6B22827971F8EA5E" modified="2022-10-12" version="1">MEKLGNLFEFFVTAGIKTNDQDVILQSCTTANKKDLAILTIFPNPVERKMNHVLADIAMWCFPLGVKTIKEGDNRFTKIEEYFKEDDVQASTNEINYFVINDFKQHIKFYCTSACFYEKMFLIMPPNDKLMQVFVPKAFCVISRYPAFIPQRSFLMQLLKIQKLRLSQNRERDKIITIKRGQQEYQIEEIKLYDFYIRTALTQCPIVQDNVEYRLKFSSSEIWMNYYVSNSHLTTHFSIFKLSNNLFDVLIFNQPIFNLYKLLLYMMLEYQIIIVSRKPAGITLFCQSLVELLVPLQWRGLFIPYLRPGSFDFHKSQLPYIIGLDRNLYEISEKPLPKELKRVIYDIDDGRILSRSDDPKCPKQYQNYFIRKMNTVFADLKFYENEIFEEGIMKYRQAFYNFKLLMLNDIYKYFSTDTSNQKSEKKIVMFDYKKFLTSFENQDQLFFLQFLKTLSFQQFVKDLYKVMEYREEFIKNNIQNVEYYFSSVLQFFDDVRFLSIRFGEQTLDDKLVILENLQNNYIKAVLERQNESSNYKVVEMKTTDFIPEYLEHLKNSSLNLSMEMQALAKFIQAAPQQEEIIVEQQQKIIQQGGPDMNQSEEQDNISQQSSKIQSDKSDQHSQFHHSLPPQEKVNWELQTIAVSNASEYRKQYQIKHLNLINKRNTFKRKNLLENEEEEEQMQLNQNTNTNNFIVTNIADKNMLSLPVKDNQSMAARSYFNKSMVPLIGDYGNAEIMVPNQDNMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1URE9</accession>
    <name>A0A8S1URE9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase S26 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0440105</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1URE9"/>
    <dbReference type="OrthoDB" id="308440at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042720">
        <property type="term" value="C:mitochondrial inner membrane peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052064">
        <property type="entry name" value="Mito_IMP1_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019758">
        <property type="entry name" value="Pept_S26A_signal_pept_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12383:SF16">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12383">
        <property type="entry name" value="PROTEASE FAMILY S26 MITOCHONDRIAL INNER MEMBRANE PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10502">
        <property type="entry name" value="Peptidase_S26"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00761">
        <property type="entry name" value="SPASE_I_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S26" evidence="4">
        <location>
            <begin position="15"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S26" evidence="4">
        <location>
            <begin position="94"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8166352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="133" mass="15066" checksum="9C4F5C42A58B8387" modified="2022-10-12" version="1">MLSSLKQILAKQKDILVVIPAFCSYYLLTQHLISFELSEGQSMHPTVKDGELVIVQRALYKIQKGDIIIAKSPVRPDYTICKRIVHLEDEIDPNGNKVPKNHVWIEGDNGKVSFDSKFHGPIPINLIQGRVIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1URJ0</accession>
    <name>A0A8S1URJ0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ABC3 transporter permease C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0420030</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1URJ0"/>
    <dbReference type="OrthoDB" id="313105at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32522">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32522:SF3">
        <property type="entry name" value="ABC3 TRANSPORTER PERMEASE PROTEIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="419"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="456"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="479"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="518"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="576"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="600"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="629"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="666"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="940"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="987"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1031"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="domain" description="ABC3 transporter permease C-terminal" evidence="7">
        <location>
            <begin position="426"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="ABC3 transporter permease C-terminal" evidence="7">
        <location>
            <begin position="944"/>
            <end position="1062"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8165076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1070" mass="120952" checksum="3BB651F70B3868C4" modified="2022-10-12" version="1">MQPQTIQRKDSMVPQGTSMSICSSLLFMIGQSWYELKRRPCGYCVSFFSVLIVVAASAVSQSIIDRAPLIFLKSAEGPAGQSDIVLTANPNYLSEEHEKSPQYLMSIYSNTARQQGYLIVDFLNFTRVKEVLGPERANQASPRIEQQVNFTYLQKTGDCENSVNKAQQLLSKRQEFGSEAHFNDLRDGQQLGQKCGGSQPHPTAKFVAIDSKKEKEIGLGWEYPFNELKETEVIMSRKLAETYRLKVGDYMLIYGEFYDFYGSYYIENYFEQQGRQFNLTNYNDTFLNLEFGELKKATQRFYLYQVKGLLDSTYGKFPDGDADKLIIVEHKHFFNNLAYWSWDKPFLKAMYTANPEDFVDEIRINIPDRFNIYMDSNYENIQGKITKYASNIRRDLGVYPCNMDLPVLQQLYDSRFGQLFLGIILNMIIVILFLLSVLLLYTLLLISVETKTYDLGVLRVLGFNKLGVVFIVLTQALSYVLPAIVAGIILSIPFLLYASSALKASIGVEIEATPTTNAVFMSLGLGLLIPLLSSYVPIKEALSKQLSFALDINRSKSTAVKIEVDLEGKSLPWGRIQFAVIASLFGICVYYFLPLALLSFNIGLLLSIFFFILCGMLLGLVIFAFNIQYLAERFTVYIFLFWASSAKKTMVLKNLAAHRIKNRRTALMYALSIAFVIFIFVGMSVQIENQATLTLQQHGCKLEITGSNGYLSPFEFENIISQLGSNITSFAWVTDSLDSYMQKLGFSTAYMTHLGQVYQLRPKIVGVSHTLFDMGYNQFLKVEDQINSKLNPVEQLYTSRGSQSAMIGTSYADQLNIKIDIDSTFLLIVYNNTFSQYHQMRASSIITSAPALKFSSLPSVQEQNVVVSLPTFRRLCGNLIDAVENYPMKRLLISVSSSGNIDTVYGYFKKYIDAKGISARIWDYRDYETSIKQSQNLIQIIFSFLTGVVMVLSFFSLVTSMSANMLEQVKEISVLRAIGNTKMSITIVYIMEAFTLVFSSSFIGLMVGFVIGQSMALQQTLFTQLPLIFVFPWQMLIIIIIIAMIAAIISVVTPSLQILSKEIAQIMRMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UTV7</accession>
    <name>A0A8S1UTV7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0470008</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000047">
        <property type="protein sequence ID" value="CAD8167252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UTV7"/>
    <dbReference type="OrthoDB" id="2443807at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="33"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="157"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8167252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="295" mass="34712" checksum="250EEA85C8D8FE0E" modified="2022-10-12" version="1">MYFNKNGLVLILSFEDLSIKVWDISKKIHGSKTCCHQAQITCLGLSKDNKVIVSTSQDKQIKIWKMENMVEDNFFDDINRLKQNLNHQKKYIQETFDKLDFSSFNKNCISLQHEANNINDIEQARLLQETIFERAEENYHNLSELRRVEMSATLRAQKTLKDEITKAGEEIKQLLKESDQQIQTINQRMIEKARCCSMAADQLSEIFTKKLRELQELKKTLTDEYETRIEQKQQNSEIKVNSQNQITITEIFCLSKTPQNSKFIGTIFTNSYIKNKTNHNLFKLWQQSQENCDKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UU74</accession>
    <name>A0A8S1UU74_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0460243</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8167129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UU74"/>
    <dbReference type="OrthoDB" id="289898at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37523:SF1">
        <property type="entry name" value="CALCINEURIN-LIKE PHOSPHOESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37523">
        <property type="entry name" value="METALLOPHOSPHOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="1">
        <location>
            <begin position="8"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8167129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="214" mass="24665" checksum="A415E9F665E28C76" modified="2022-10-12" version="1">MQNYQKVILCASDLHLNFETVQLLIEKEKHQKFDAVFLLGDFLNLQHQQGEDSELDNLRKLLSPFKCFGCPIYFIPGNHDSDELLQDKFIEEGFINIHKKSLQIRDDLWIVGLGGSIPGYNQKGLIWQGYPYQSDKEYQEDLEKLIIPEEGKIILITHIGPSLSQTTISSENGWDDVIKSGSDSQSNLIKNNQCWSITLWQLQYYQNIKQSGQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UV45</accession>
    <name>A0A8S1UV45_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2 NT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0500077</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8168960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UV45"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019448">
        <property type="entry name" value="NT-C2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10358">
        <property type="entry name" value="NT-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51840">
        <property type="entry name" value="C2_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 NT-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="197"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="343"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="416"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="804"/>
            <end position="842"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8168960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="870" mass="102501" checksum="DAE33B7ED84DDD57" modified="2022-10-12" version="1">MNRNGYRFQVEATILNVQLLVHIPCKIQVVIKYGSQKLETRSHYELQKGIAIINETLQFNINANLNQDGNLEEKKAQLIVILVTEKGMKNAGFHSLDFAQYLNQSNTQYQETLALDKCPDKKAKLLINFKITKIGEFEKDETKDHFIDLTQVSVSPSGLESHTNQNSKDASEQILLLSDNKIDESGSKQQKQENQLINNKDIQINEQNLQIQQLKSKINDMIEKQIHENMINKYEQQLQDQENKYKLIQNNNEQINLQLENQQQNLENEQSKQSMVQQIQNFNIEIQTLYKQISDKNQQISELNQKIEILENQQSKQIGFGQTLININQQPGEEIINETGKLIEELQSTLRHKQQDYETLQQKYEIELQKKDELIQNLQLRLDEVELKEINITRSSYVSQTQIIQSEKLIYDTNIKPKQEQQINRLKNQIQIWQSKYESSHKELSDFNNQIQHLQRSIEDVQIFLEDQEDLVEPLFLLRRAIQEKLQNHQLIEQHLEQRVQKLEQELEVCQYQMKLEESQQCSDQTFEPQMHMFIDEVSHLNKALQQKVEQLKILQQQVDQYKLKDLEQQKQSEEAKVQLASLRTNFAKVKQQLAETMQEKVILQAKNAEQQKALKEKNQEIQKEQELIAELEQFNIQMEEALSQTLDQYKIQNTQMKQAYEKEKKLRSELQQQIKSLKEDQTKNLKNEQPKIEEKQQQQCNCKDKIQFYEQKIAELEFQLSDFQIQKLDKTIQRNSIAQIRTSILINHRESLAIQQLEEATNQLNLINSNQGNEKLKPKELEDQNINLTDDRFKMSKEYQTILEKALQTNTQIENKYKNQVKELEEKIEKQTQCLVRMKLQIADLYSAAIEIGGALLVDKLHIALGIRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UWP3</accession>
    <name>A0A8S1UWP3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0500148</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8169075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UWP3"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001435">
        <property type="entry name" value="Probable myosin light chain kinase DDB_G0275057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001500">
        <property type="entry name" value="Probable serine/threonine-protein kinase fhkD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="11"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8169075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="314" mass="35689" checksum="D62C3A4524CDF378" modified="2022-10-12" version="1">MQQNEDINKVYTLEKTLGEGAFGVVKRAVKKSSGEHFAVKIINKENLSNEDLLALQTEVEILTQIDHPNVVKLYEIYEDDTYFYMVLELMTGGELFERIVEKDHFSEKEAAATLRPIIDALAYCHKMGIVHRDLKPENLLYSTMEPGALLKVSDFGLARFVGSEEVMMTQCGTPGYVAPEIINGKGYTEAIDFWSVGVILYIMLCGFPPFYDEDNDKLFQMIKTGNFVFPSPYWDQISNEAKDLIKGLLTIDPAKRLTTEKILKHPWLVNNTHKSIVNLQAKLKDYRASKKIKRIANVLTIARGWGKMAQVKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UWU9</accession>
    <name>A0A8S1UWU9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0480152</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000048">
        <property type="protein sequence ID" value="CAD8168029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UWU9"/>
    <dbReference type="OrthoDB" id="285207at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="361"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="390"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8168029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="542" mass="64444" checksum="04D36C0971BC1E02" modified="2022-10-12" version="1">MGSCQGTTQDIQQDLRMREIMSQFQVPMPYTEEYQIFQTLKWLEIIQHNTIPNFGLIYSKAADSQNKLREFVKKWRTKYEQHNHPNLLSIHNISIIESMKVFIPYTEIILLMDKPLEPLSRHRKVIMKQQFNQDEITFLLDCVVAAFSFQQTNKIYPSGFDIDDIIIVKTPFNIDVYKLVDRYQKPIRQNLFQEFIQLYDDKQTLNEMKKTAFLSPAQIQAIPRKDKYLKHNRYKSDVFNFGLMLIYLIQAKKPEVYDWKRWTIDDFALRKVQDDLITTNFNEQILHLIEMMLKVDEHERPDFIQLDEMVNKLFLDLKKHRLLEQYQPQEFFIAGYNKPSLFDNLMNQNIFQSKYPQSPTMIKSSQHLSKNQQIEQQKYKQQDTSQASIKDQKWQEGKTQETKTGFENSVTQGKLHYSNGFYYIGQICNRRRHGLGTYYDSENQKVTEGMWVFDIPEGEVTFYNKPKQELEKSHKNLARKDWIQYKGQVKHGQKHGIGVLYFADGSKFMGVFSCDQAHGKGQFEYQSKECQIGNWQHDLYIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UX86</accession>
    <name>A0A8S1UX86_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MYND-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0490131</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UX86"/>
    <dbReference type="OrthoDB" id="432970at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052298">
        <property type="entry name" value="ZMYND10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13244">
        <property type="entry name" value="ZINC FINGER MYND DOMAIN CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13244:SF7">
        <property type="entry name" value="ZINC FINGER MYND DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="5">
        <location>
            <begin position="395"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8168472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="457" mass="54645" checksum="F392C5B7B9E1E5A5" modified="2022-10-12" version="1">MNIQLTDILTAYEAEHYIESLQIFEITELGCKKWFTQDEILQKLNFQAHINAITRSDEFIMEAFCTFDKIKPLIYDLIMTEMWKQYVFPYLKSHFTELSSIRSYTILQHEAIVCNLLQICLFHRTSIESSEGYILEVVDYCNRKLTALLQKPPAKKIDKKNIDYYKNRTKEDEIDEQFQNVEFQIQMMCLSIIRFITDHIKHLPINILHQIIVENDFFFLLVPLIEEKPWLRINHNNEREVFEQSKWITLNKEDYSKLPKIEAQLWITIYNLFMDPESRRKYELNDFKKSNLLRLRKFMNELLLDQIPQLVDMLRSLEELSLMQVQTQSKNTIVVQQLPELRMAICKDKNWSSIAQKQKEEYFQINDPSIKEDIKRMAELYSNTVFEGIIDGFKCEKCTKEATKRCSRCKQVWYCSKDCQVGDWPKHKLNCQIINTQKQEDTKQVDKQSDKLLDQID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UZZ4</accession>
    <name>A0A8S1UZZ4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine carboxypeptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0500160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8169099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UZZ4"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000253">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF201">
        <property type="entry name" value="CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035793580" description="Serine carboxypeptidase" evidence="1">
        <location>
            <begin position="18"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8169099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="473" mass="53342" checksum="EA2DD39B3EA4C68E" modified="2022-10-12" version="1">MILSLLCFIGFVAGAPAEDLVDGSTWAKFKIPYSGKMYSGYLPIDDAGKKQFHYFAFPAFSLAGPLQATFPLVLWLNGGPGCSSLYGAMVENGPFTVELGTNNFKQNLFTWLNFANMFYLESPAGVGFSFGNTTTDDKSTAKDNLKAVLEFFKKFPEYKSIDFYIAGESWAGIYIPTLANEIIDYNAKAAAGDKIRLKGLMIGNGCTDPTECTDLGFNFPIHFYKFLHNHGFISEKLNDKIVAMTSKCHMKSNPECMEIYGEVMEQINGDDDFYFNPYNVYGKCYQLPFYNEKGQMVKEKRFKLHPMKEGVVGQVNECSESEALFLYLNNAAFRKALHIRDDAGYWNDCSYIDYKKDPGATYYLYPKLLKNGIRILKFSGDVDAIVPITGTLYWIDKLQKELNLPTIEEWRPWYKPGEKGSEQQNAGNVWEIDGLTFVSVRNAGHMVPMDQPEAASIMASHFIFEMPLPSDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V232</accession>
    <name>A0A8S1V232_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RPAP1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0540062</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8171001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V232"/>
    <dbReference type="OrthoDB" id="290524at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013930">
        <property type="entry name" value="RPAP1_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08621">
        <property type="entry name" value="RPAP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RPAP1 N-terminal" evidence="1">
        <location>
            <begin position="51"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8171001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="658" mass="78315" checksum="12DC3961F110468E" modified="2022-10-12" version="1">MKQSLFAQRMKLKKDIQKPDENLSFPQTFKVDIPPQPNRAQFHGLSEKDYQQIDEENLKAIKEMNQEQVMELQKQIRESLGDNLCKMFESGDIYQIKQQKQMQLEINQTITKNTEVFSKLIDEDQITISQIQELIKSPEMSSVKLALQKIDKVIKPQNVLQLESINLSDYLISVGKSTNISVSLFSFDLLYTYWTQIVPNCWNPIYNLKETKEIEDLYIDLPQMINQLQHHIHYTKALDLLRIRNILSQSNSKYIIEQQSIINIECKIKSIILLQQFQELQKIDFISQEFSDFLLSIIFYYFNYLLDSQCNIIISELSKLQNSNLKLAIFYQYQQRFSCKLIHEPLILNIKKPEINQDIQIYEIVPFQSVLAKLFDSFNLAIFSGQDVITCLKQIVSLIFDIRYLAKSIISRKQRKIHKWRLLNILRSIFQDQFKNEANKRTSIKENNEKYQLLELIRQQLKIEFHLRDEKELVFEASLYSGLSQFLSQHEYQLEVVQQYIDCFNDIDFEIKSEFVLLKLYKLLISNMELPKGLINLYKYLLNKINIKELAVLLNKNVSYFEKLLQKCQYDSYYNETYVNVLFLFISPHFNPDFHNLLFQEFQCSPILQYLSSEIYMKHLNYFTIKPNKGLEIYRQNVLDVSSQNEFIQKWQAEISQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V5J7</accession>
    <name>A0A8S1V5J7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0550263</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V5J7"/>
    <dbReference type="OrthoDB" id="287672at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000015">
        <property type="entry name" value="Oxysterol binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF148">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8171887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="867" mass="101752" checksum="836C94BB71A51B0B" modified="2022-10-12" version="1">MLDNNKNALMSCSAFKESGVLKGWGERLLIYDLIQQILRYIDVKGQTQKQLLIQNYIIHPVAFHQGKWAIKFKTQQEIEPKVARFGWNNQSDALQWYDFFNGKYLAIQIKKENQEKKLQEWDQFMPESERVINQGVPSMAIKPSQVSRMRQMIPLLPQFLQNDIEIILENTHENLQGFSDIHSFKILFQSQNKQLYQDPNDQLHMRFFLKSEMPPSRIFDELSIHTFVDNWSPQVSIYQIYPTKKDCAIIFTEFNMEQKQQHNNNKFKQNKPRSLKDIEHEFFSQDTFSKQPKSQIPNQIEKIRLIYTQKHFQIDENAYCIMKKYIGNEIHHNQIDKDKVEGTDEYKITRSCVLIQAKQEDKFKTQIIEDIYLECENREKGEEVMKRFLLNLENIDNQILEADHYIKQQTEHVLIKNEDDDIDNLQISFIQLVPEIPRITSEQHQQQSLQEYKEILDLVQTGQFIQKSISRQFNQNILNGLDKFLETTKEEGHFLLTKYYEVDPKKGGLIYTNKKLISDQRSVLLDIIKRMGSNLLSGKSLMSVSLPIQVFEARSFLERMARGQGHAPVFLEKAAQTTDVFEQLKLTISFHMASFMMGVQQEKPFNPIIGETFEGRIKGCPIYLEQSSHHPPISNYIMYGRGYKLFGTFCPLVNMGTNSLAGEQQGHSQIHFQNTNLKYYYMAQPFMLYGVLLGQRSVNCHKRSYCFQPEHQLLVEITFNPKDKNAGFFSSSSQKIDQFIGKVCKVTPECIQKCLKAHKTNSGIKLKILPQEILDTYNINIKGRWTSFLQFDNVTYWDIEIHRPYILELESCPLPSDCLYRLDLLYMKMKDNSKGQDVKEQMEVDQRIDKQLRDKFGVKTKKKNKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V6A2</accession>
    <name>A0A8S1V6A2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0560169</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000056">
        <property type="protein sequence ID" value="CAD8172217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V6A2"/>
    <dbReference type="OrthoDB" id="1893551at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF122">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN SKYWALKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="1">
        <location>
            <begin position="146"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8172217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="321" mass="37716" checksum="2D7F7F5D466A029E" modified="2022-10-12" version="1">MIRLTNRVINQKISIPRFQKILKIQKCIRRCLNLWFQKIKFNSWKEKENKRFWSCLCLRPQQPIYCGMQRVKMAISLRSPQGFTPFGGWTDVLINIKLSYDAPYYILLLLFMVPKSPSLNFNIQSAKQFMMQAVLEQKFDKLLKPNLLKDNFWIKIVYILQEKTNKVIKQSNLIYQGTRDGLNNQQYWNKVNGKGNLLMIFKSKSYYIFGAYSPCKWESCNGKYIEDNTLSSFIFSQTHDQVYPLKQDQKQNAIYCNSSSYGPVFGGGFDIIIDSNFTDGYCKLGSSYQFDQYKNGSEDPYLFGQNKPEITECEIYELQFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V824</accession>
    <name>A0A8S1V824_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0580062</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000058">
        <property type="protein sequence ID" value="CAD8172811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V824"/>
    <dbReference type="OrthoDB" id="311745at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035824233" evidence="2">
        <location>
            <begin position="21"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="32"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8172811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="240" mass="27703" checksum="31D6AA964FFDCD7A" modified="2022-10-12" version="1">MKHILISFLLITIAFSSLRGSERKNKHHDQNSRSYSESSTESYDYSNQSSDDQYYSKESNGEYSSYSSSEDSDSDLSYSSSSSNDSDSSEDQQKSHRPRPDEFTRSVFKTLKQIEKGILQDWRLTEDPVLKNQIRILLIQNLENLLPIAQTPVEPIEIQSVSSRIPEENNQNHQRPPHPRPPQPPHPEPEDEFANQVFDTLHQIAQVILEQWNVEKDVEVKNQLRQNILEQLGALLPQNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VD45</accession>
    <name>A0A8S1VD45_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0610245</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VD45"/>
    <dbReference type="OrthoDB" id="300502at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8174717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="408" mass="49387" checksum="AD915D05EB897D93" modified="2022-10-12" version="1">MKPNQKVLDHLKKYDYYLPLHLDYVQKWFYEQMVDNLGLSESFRTSFLSQISKIGQYAHNEQQFRQLVRDSWFCFLNKKPLNFICIMIDGQEQNAIKEFLLNVLIQPKQYDITYIDELLQIYEYYLQGNGGVVNIINRTKFRMILSKLNCSRFILNQDERIQLQIKQYMFDLYLKNIEKIDEKERINVEFITRTNIFNKLFQVLFDQIEGQGNMFNHLKQIVFVMIEEQLNILISFQILKSFYQMYLDKEKLLFERIDSRVNFDHNRIARELNQKYKEKKDLVEALKSIVQKQTQTINYKLLLRELVTKDIYSKENEQILNPILKEFDINNQQKNKRQQYIDNDLIQQIKLLGFNKLDKRNENEYDNLNFSINIIDNNNIIVINKRNNQKGKCQNIKELYLFMKNSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VDD4</accession>
    <name>A0A8S1VDD4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calmodulin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0440121</name>
        <name evidence="8" type="ORF">PPENT_87.1.T0610112</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VDD4"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000560">
        <property type="entry name" value="Centrin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000651">
        <property type="entry name" value="Centrin-related-protein,putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="25"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="103"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8166384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8174465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="176" mass="20173" checksum="85B98CE172D561A5" modified="2022-10-12" version="1">MQGKAKKGQRKAKAGHSFRQELSEKQKKDIKEAFDLFDVDGSGTIDEKELKVALRALGFEPGKEEIQNLKQNLNNNNDSKENKNTIDFNEFLQIMTEKMNAKESQEEIERAFHLFSQGNDNFITFENLKKVALELGETMSDDELKLMIQEANSKNPSQGYVTKDQFYDVLSRATNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VGP1</accession>
    <name>A0A8S1VGP1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0640193</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8176147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="295950at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="273"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="962"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1155"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1262"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1472"/>
            <end position="1494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8176147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1539" mass="182368" checksum="F8DB1674E8238279" modified="2022-10-12" version="1">MPFQIKLFEKQVVFMLSHLLTLEYNMPKLFQLLLIIYSVTQSISYSFYYQYTQDEQLKHSLTKITLLTRLYLYIDNPDIKSALFWLLVVVQNLIFLAASIVAYKNQYVMGALSFWTRFISVIFCFYIYYFSTAFFPVIFEICLFKSFDLAQFCVSIVVMIETLMIMILGELIFRRGLILKSTSQLYINMTTYSYIIKILKLMQIIFFFFLQRQFLSQIIQCVINILIQICFIAEQIRVKVYAQKIYSNTTIIFSSIGLVLSIDVLLTQSTNNNNYWILVCIIVIKILMFCEEFQIDKISNYGIHELKYLFTFLDLSNHEKSKTKLQIFILYKSHYQNCDNKNTCFCTKYKNDQYVAIIKIIESKIEEKLHGKIEISRDLICTDYAQILFRSQQYVKAQQHITRFLTRQSSNYEKKQNPLSTITIFLLEYLKGEVLNQVMIQITEKIMLSNQHNKLLNESLKSFVLNENSDSQIIFNLKKIIQTKIQFYYNFVEHQNNSFGNVNYALQFIQQIQQFKEMLLNRYNQFPTSSNQNILKYFTLHILNDYIDSFNINRGKAFEDDKFNQFSGQMYHKIFGINPAYFITELNSELEVIISKKSQRAQYLLEQWKVQQQEQKYHQNTELNAFLPEYVQLKHSSLIERFLSEGQNKYYQQQNLSFLKQLDKVMMPIQIMISMNQTNYDKFSFLTFFYESLDYRSYIVVDKHLEILNISSNISESLLINESKVHSSCITKIIPNFQQLISTHQHQFYNQEIKLVKEYHHQKVPCSFTGNIKIDRKFENQYKCYFVELDNIRAISKKFKDENSTLYMASLTQFPGSTQKLLNINSELNDSEEKMIPYIDIENHPIPENSLCIIQQEHHRQTINQTDTNLVCTLQMVEIGKSQNVFEPLSQKRLLHSSKYSHDEDMKKQVNKIVEEDEFLEIGSTSSVTAIRRSKYYKKFEIITILMKSKKQSQKIVKMNKFIFLYIFVILLGLIIQILMIQNLEQFVEDIKTLSIRYDVVEPYESFYVSRFSQVNYREQLSGGHINASVYAQLTKYPFSTFSLMYDHLRDSMYSVLERREIDQLSENEYITINFLNSSYVGYTKNVTIRSLANILMNYQYDFKLGLTIRNVAFDSPFFYFTSKNYLTIKHTFDGLNKIVLDETLQRSKLEKEKWLSVLLPFITISLLLCIIIINSYREYNQIMNSIFQYLMDLDQQILDDEKKRLQKHLSIVNNELEKVKKFEFDIDAIDAELEESNNLTNRNNIKRDAKRFKITNNYLKTTAAIGFIHFLLFLTFYMTIFFIIERYFEKYDQTSIIFQRISDLGVDIPTLYAQREVLYRRTLRYFFLSNAEIDGVYDVLFQAYNKVEEFSQLNIDLSSNDYLFDENAVSFYQSINTGNLCDFQTEQFKQLSNLTCGIAMNGNLLKGLSQILPYILYTLKTQQYDSKNFTILPTNTRLELEGATIIGNVMSTVISELYDNLLISCLRLINFTQIPCIIFLVFQSICIFIYITIMNEKHKQQFQSIKQSIFLFPRQTLIFDDQFYRNFRSIIKDEGIQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VHA0</accession>
    <name>A0A8S1VHA0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0600239</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000060">
        <property type="protein sequence ID" value="CAD8174126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VHA0"/>
    <dbReference type="OrthoDB" id="59449at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8174126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="260" mass="29673" checksum="E5BBAC5D83F73822" modified="2022-10-12" version="1">MIPKVVGYASFQSYGDLPYPSLKAGKRTLDSNQIWEQTVRNEDKESFYKNSNYHYSNKQVSLDSTVQPPHYKNVYKANKARLESATSSDFFQKTGNSAMTPENPLLKTGCEHWKSTYQASIVDPYAKNRAMPPEWSLNRPPYVVETKVGPSEYKKQYGQIGENPRERLNQLDETHPRAFDELRLGTSQEAQHIPGYTGFIPSIITNSKAMEHGNGTEPRTDFMKTNLKENYHTKIPGYAGHVPRSVVNQRDQPRQSCFSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VL67</accession>
    <name>A0A8S1VL67_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0660112</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PPP phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDO010000066">
        <property type="protein sequence ID" value="CAD8176995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="429197at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="5">
        <location>
            <begin position="117"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8176995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="189" mass="22088" checksum="FC6482211C71B8BC" modified="2022-10-12" version="1">MSSKITTPEANNLDNQIQHLYQCKPLPEQDVKLLCEKAKEVLIEESNVQPVRAPVIICGDIHGQFHDLMELFKIGGKAPDTNYLFMGDYVDRGHHSVECVTLLVLLKIRHRDRITILRGNHESRQITQVYGFYDECSRKYGNSNVWXQFCKLNLQMHQILFILYKNINIYTMLLQSYKFSNQQETKQLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VLL6</accession>
    <name>A0A8S1VLL6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0670040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000067">
        <property type="protein sequence ID" value="CAD8177135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VLL6"/>
    <dbReference type="OrthoDB" id="323908at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8177135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="73" mass="8883" checksum="4B097A2447E98E75" modified="2022-10-12" version="1">MLNLKTKKHSFSIQKRQYWTQNDNKLLEKVVKLHNSDSKTILLEEMEVNVHQDELRQTGDFNKKYIQWSHQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VN27</accession>
    <name>A0A8S1VN27_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0660121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000066">
        <property type="protein sequence ID" value="CAD8177009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VN27"/>
    <dbReference type="OrthoDB" id="9978460at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBC core" evidence="1">
        <location>
            <begin position="34"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8177009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="178" mass="21098" checksum="F33EF3564FB1F9FA" modified="2022-10-12" version="1">MTFYQLKNSLSVQIFQKLIYLYNCQYYLSILNLYVSKKNQESITGSPKELPTNCFVNLFDNLDFYKWKAKILSCEGSFYQGCCLILKRYFHGLSFKTTKICYIPPIYHLKINSQEQLSLDLLQNQWSPSQKISKILSVIYDVLENPDDPLDPEIAKIYQFNKQLFIHDVQEWIRKHAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VSQ7</accession>
    <name>A0A8S1VSQ7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0720223</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000072">
        <property type="protein sequence ID" value="CAD8179791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VSQ7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8179791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="128" mass="15034" checksum="B7CB44FC671872CA" modified="2022-10-12" version="1">MYCNLNIIIYGIENKSIQWGNKLKVSYVQDPKYLDTSLKIINSFLVKIFKSSFGVYRFYQSSSKTTNAICLVVFKSILQFLMILPLILAYFGQIYRIDQEFKLITQKSIQTNATRHIQIIFQIKILFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VUH5</accession>
    <name>A0A8S1VUH5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0760093</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000076">
        <property type="protein sequence ID" value="CAD8181364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VUH5"/>
    <dbReference type="OrthoDB" id="293518at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="224"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="97"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="228"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8181364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="320" mass="37717" checksum="E3BCD15E8929C3A3" modified="2022-10-12" version="1">MQSIKQLLIDILDNIDQVLSFSFQDVVESYQQINKLTTKEICGQSEIQQYDYILQIVAYLNRIKILFEEQLDEHINNDYESIIQNLEASIRSHIRVEQQQKLQIEIFTQKLEEITREKDLIIQQQQDKILSLEQIIKDQNKKLQETSPVEIIHKKTPSAFDRLGKFVQNKVQKNIQNSTQDTNFKTFLKAFHTDADRSISKGRRQLSAKRELGLQRINDETQLYPESKITETERQRENVRHKLQRSDKTNELQHQSPEKQLYLNKYKGLSKFDIIKQEQTISSQSFDQVGKNLIALQKQISNGRLKNTQGTQLLKFLQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VUM5</accession>
    <name>A0A8S1VUM5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0760129</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000076">
        <property type="protein sequence ID" value="CAD8181414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VUM5"/>
    <dbReference type="OrthoDB" id="290826at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8181414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="105" mass="12256" checksum="979CBFE010DE36CE" modified="2022-10-12" version="1">MNDDVIVKKIKLNVGSYVMPQDDFKMDSKSLMQTEAQRIFNQYSNVVKEEKKLQVFSQITQQTSGFIKRLEEHRQILKTQIELQLKICQGNILLQRALSGIEIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VUT0</accession>
    <name>A0A8S1VUT0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0730070</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000073">
        <property type="protein sequence ID" value="CAD8179935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VUT0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8179935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="63" mass="7696" checksum="063FEB2353A377EF" modified="2022-10-12" version="1">MFLEITFMIKMKSLQRQAIDSQKINQETFFRIFFKSTIQIFISPKDYPVEQIIELRQIHLFII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VV17</accession>
    <name>A0A8S1VV17_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0770027</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000077">
        <property type="protein sequence ID" value="CAD8181698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VV17"/>
    <dbReference type="OrthoDB" id="293090at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="174"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="246"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="343"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="426"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="690"/>
            <end position="717"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8181698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="769" mass="90863" checksum="7E9017F63F081BCD" modified="2022-10-12" version="1">MQHNYKKELKNIIDKQIIFIIFICVIKVFVKLRRLSIIFINKLSMNLSMQPRTISPTKYSQVLASQDFSQPGTVKKAHPLNNQSPYIHSPVQNKMNFSIVSEDAENYRIKCYELERELAKYKADNELQRLVQQKSGDLEYKVVEVLESNNALQGQLERAQKVALQRKSESEMWKQKYEAQMGSLMQIRQNYESEIKALTLEVQKANARSSALDQEKNRIISDQRNVADNQTTQIQETFKRSNNSQADMYEAQLKKLRDMLEDRTLQVAQLQSQIERQKVESQDTQLRLMNEIDITKSRLSAIQIDHQNELLAQKQKLELYQETNLRNQQTAHENQQDVQGSEIVKLKNLLEIKTQEIETLINQNQKQKLQSESEIQCLRTEIEMLRRQILSNEQLHQQENHNIQTNLDNIHNNDTTNLKLTHENQLQALHKEILKLKEIIDHKNQDIQKLVVEKQQQKDYYDGEIQRLINTIEDQKRKGILLENEKNREIADQHARIERLTIQYENMQIDLQKQIQLLNQEIINLKNLLDHKNQEIQQNLKNYSQIKLEFEDKLRNLQNELELVKLRSFETEKSKLQEIQDLKSLIKEIDTQNREKQKQLEQQIDHQKYEIQKTFDMYNNKIKECENLTIQRNNAELIQKRIQDDNDKLIEKLHHFEQEKNLEIDELRHKMDSGAAYQFENLKSAYNTQVGLLSDQIADLQNQLAIKNKELTEMFEKYTILDKSLLPETLVSRAAYNSRIGVPAELLANKSIHDATLKSQLRNSPIQRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VVQ9</accession>
    <name>A0A8S1VVQ9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0750005</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000075">
        <property type="protein sequence ID" value="CAD8180737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="28293at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002895">
        <property type="entry name" value="Paramecium_SA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934:SF6">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_176, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38934">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01508">
        <property type="entry name" value="Paramecium_SA"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="SMART" id="SM00639">
        <property type="entry name" value="PSA"/>
        <property type="match status" value="23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2674"/>
            <end position="2692"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2735"/>
            <end position="2760"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2772"/>
            <end position="2797"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2847"/>
            <end position="2864"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2876"/>
            <end position="2897"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2909"/>
            <end position="2931"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8180737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2962" mass="340082" checksum="0EB3A0580010445C" modified="2022-10-12" version="1">MKEMGLEINIFKVEEKNFNRNFYSLNFCLSLKQIIFVVLFKLCKRESFKPVLFILTQKNIQIFIEIEFINQKKLINPMYIIEKQAQKDNYRISMLQNPKNSSLGLRMIFLAYLKLLLLQFLSIRGMGISEICVCGHVQSENDCKNSGFCIWQNDACILKFGQTYNKIGQDSNSCKNYAKEDCREQKQCGFYLGQCNTFQDCSVFNKDHCEESSYRCVSDSKKCIERQECNNYKTLYECANKNQNGKYCFWVQEVERKCRDAIICEELPIYLTSHIMCKEALQGCTVNEKGYGCIKQMDSCTQYYNDFQCIESKSESENCFWDLKNSKCVSKVCENLPFSQDYECKSYLSQCTSNGIHCIKRRQCIDAQNKFGCVTDAEGNKCEFHKNQCKIKTCDTALDYYTNYQQCQGYDNFLDCVTAENGGCKQRPQKCDGYGAEVDCYSIEEQDCIWYNNKCEKRQCQHAPVFYNLDDCMQYGNCIGKIGGGCQETPQICDQIFEEQFCKFNYNKDKCIWLDNECTLLECNKLKLPTFFNHQICQKASSYCTFNLEQFGCVDYVCENIVETELCSIDSNGTVCTLNQGCIDKKCQTAPPTYVSNEQCEEWLPYCTVNVQVLQQEKILIGCVNKYNDCLSALEEQCQSTFSGINCKWDSVGQKCLYKVCTDADPNQYLNNEDCKQFKVISGTCIIGSSNFGCQQWPNNCYQLISQLQCQLNLQDGTKCFWTGSSCKILECTDASIINYTNNIECNSWLDYCIYNSISGGCMNRPASNDCTLSPNDNMYDTHQECIAWDPKCTVVSTFNAEGCEQKKTNCFEFIRQRNCKTTLSGQNCYWDDKEQKCKDEYDDNDCNKRIYGDLSHQDCEKFLSKCTVRKIQTSCKSLSKYCDYLLEQQCVITIFQQPCKWDLKNQRCKDVECYENITAETEAECLNFRKQNQCQLKIQTDQTYGPGCESRPFYCEQITNHIKCKLTLTLQNQRCYYFDFKCQVVQSNQCEVIQDSKSNEVCQLYNPQCILQPSGQGCYSIFGCSDLSNNVCNNAIMKFNNKCKYSDTCRLDDYCYNKYASLSTCQDKKTQLGQLCYYRTYCYYGCSRYCEQKTGKKYLQFELSTSFSDKSKLCQEYSSNYKYDTNCNCCVIMTSCTQQQGGQQLCNSSTSNNKCGYNFQTNTCETRKCEHLTYVNYAVISDQICYDWKYNCVLDATGCTNYTGDCTSIKLIYQCYQQQCFWQDGKCVNHVDCQVNTTAVLNRECLLVNAENCRLNYTQGFGCSFFDCSMIKNLMICNSTKLVDGQSCNWINGLCKSKICSNFTIQSDCESSYEQYSIVTTKCFWCIYNTTKCSNQQYCSLSSMILPKSHQDCNSQNFQHTIDISMSSKCTIKKQLCSEYIYEDACVSTIDGVECQWNSNICINICQAAIFNPFSTNLQCFNWHSDCMSSTGSQCELLDCSNLAIIEDCTIYTKKCFWDGSICQTIGACNNYFTNELCLNTKSSKGIPCFWNNTQCIEKTCQNKPTPSSSNLDCDSWLKNCQFNINTNQCVEDCTSADISYITHNQCDSYYDNKSCTVKLDIIQCVYLPVSCNLAKKTQCYIDRVGNQCYFDLTSNKCIILTCTNLSASFITHKSCNEKKQDCTVNTTLNGCQQLNDCSSYLIQEQCQIDSNKTECEWILSQNKSQLITFSAFSCHQYFGDTCTVNSNLDGCNIGQLSCKDYTYQQCISSGXLPKSHQDCNSQNFQHTIDISMSSKCTIKKQLCSEYIYEDACVSTIDGVECQWNSNICINICQAAIFNPFSTNLQCFNWHSDCMSSTGSQCELLDCSNLAIIEDCTIYTKKCFWDGSICQTIGACNNYFTNELCLNTKSSKGIPCFWNNTQCIEKTCQNKPTPSSSNLDCDSWLKNCQFNINTNQCVEDCTSADISYITHNQCDSYYDNKSCTVKLDIIQCVYLPVSCNLAKKTQCYIDRVGNQCYFDLTSNKCIILTCTNLSASFITHKSCNEKKQDCTVNTTLNGCQQLNDCSSYLIQEQCQIDSNKTECEWILSQNKSQLITFSAFSCHQYFGDTCTVNSNLDGCNIGQLSCKDYTYQQCISSGQMNLKRVSCFWNEEKNNCQEKICENAPSNAQTHSECSIFLSTCQKGGCRIKGCFDYNYAIDSACASIFEDKRCTTNGYQCILRMACEDVKMIDGCTYDIHLNPCVWIDEKCYTKTCSTAQVSLNKYEECNAYLPYCTVKQEGGCTRKQSCSDYYIKEACQTDSENQECIWDNSVKKCFSNQCIGYCGDGIVSNKDEQCDDGNYLPYDGCYKCLVQCPIGCNICNGQQCKECQKIGWYLIESVCTSKCGDGHAVDNEQCDDGNNIQFDGCYECSYSCHEKCLNCFQGQCLECEKGFLENGPFCHHICGDGYVVNQLEQCDDGNLNNHDGCNNTCQVEQNWKCQTDNFISFCYYVILPKITLTKLSKKDVNIQEIKLSFSEPVRLNEKAISEEQFLQLIVIEIINAKNNQYDFETKPMIPINTELADVAYKIIVYFKTNVKNPVLKVAVNSDNIVNLQENSLFSKEAKLELKSSYKISTDQQSLMFKTSMFSRIVLYLIFVTSGISCLFGNLEILWNLLDMLQQLSYMKFHNLQFPDNLQDYFEIFSIGSFTPIIDKLQIDLYLQNIFNFQAPVIKAKWKFEYYQINCYFLQTFETLLIMILLGFTYFILSYLLYKLLIFSKYNNWPAIFQQDHPNSSFKVVKFIFSIQRYARKYYQYFIYSGLIRIFTSNFYELTFAAILQLVNIDTKTTLNTIIFFLALITLIFNITLLINFFIYLSYKTKVAKTLKVLVEGIKNKENQGQKQYFTILLIKKTLFITNLVLFQGLAGAQSIITACLSGIFSYYVFLQKPFENNFENIKIYITEILIMLNVIIFSLYEMLNLNQDQESAQVLGWINIGGFTLILILTLAIDIYQQLLKCLKLLKGKSCFRINRKKQLKSRILFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W0G3</accession>
    <name>A0A8S1W0G3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0780126</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W0G3"/>
    <dbReference type="OrthoDB" id="285451at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8182303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="143" mass="16913" checksum="CCBCFEDEFE42A740" modified="2022-10-12" version="1">MDLRNKKSQSHHRITSYQFLKWGQFKQELPKLAMPPTPRKKNLSLDLLSEVRNYQKQKIVIESGQLTSRGLRQMLKPNKLKWVNQEVKLDSQSLLRNKLTVADFFLNSKKGKTELMPFDLIQSDSLKQFLGRLKCHIKTRQYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W204</accession>
    <name>A0A8S1W204_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0800121</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W204"/>
    <dbReference type="OrthoDB" id="444540at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051581">
        <property type="entry name" value="Ca-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34524">
        <property type="entry name" value="CALCYPHOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34524:SF6">
        <property type="entry name" value="CALCYPHOSINE LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="24"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="60"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="227"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="263"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="331"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="367"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8183181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="494" mass="57858" checksum="701453652E522FA6" modified="2022-10-12" version="1">MDWRNKLDLIQNKIRGQLHARKIDDLEGVYQLMAEFDRDNSGYLDKDEFQKFLSKIGVFLTTQELRAVYDRYDQNKDGNIAYAEFVNLIRENMSEKRINVVRNTFSFLDQSRQGRLQLESLYRLYQAKNHPRVRTRQKTAEQVTKEFMNAISKRSKDSQSINEDEFLNYYADCNATLPSEKEEYFTDLLTSTWGVTSGADYVSPERLAQLEIILFEKIRQKTVTKDDEGKTAKKAFMYFDLENKGTIDIYQFAQALQKFGCVFSEKEIQALFNKYDSDKSGRLCYDEICGLIALMGSGNNANVNPVFQIARAPPQDTLNRIRNDLIKKGQHSVIRMATIFTNSDKNKNGTLNRQEFQWAMKESGFLLTKTEYDNLFRYFDKNCDDEVSFVEFIAFLRKPLSQYRIDLVNQLWNKLSNGESQINSQQFNQSYDASKSHDVNTNIKTVADANKDFHDIWKDVQHVTQQHFLEYITDISSLIDNDQVFEKFIRYAWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W8T4</accession>
    <name>A0A8S1W8T4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0850034</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W8T4"/>
    <dbReference type="OrthoDB" id="10251741at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="325"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="420"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="462"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8185337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="536" mass="61965" checksum="4CB55F29498BBC2B" modified="2022-10-12" version="1">MNDLFSKANSIKCHIKHHSDLGLGISVMCLDEKCISNKKAICAICAKESHINHKVISISKIDQCITEYQERWVQNKLKQQLVNDFETITEEIGQSFLKYGSQFSQILDEINQVQNIYKTYIQCEEITEVVLQKLGQELQELTRLDAGSLNLKFSGQKQISQLKQFVLEMQSLNILLNKAQKSNEMLQSIILQTQERDQLKQYLENLEKLQYEQTKDQVINAVNEDSFNLCDKNLIKLKLDYYNSVTAIDVTNDGQLFAGGSNWNNKVVVYDLDSMIELKCFICQSDRIQALKFSPNKKWLLVGSGRQSDIWMWDINNLDSRPLIFKGHNEGINRFSFSNDSKYFCSVSGDRQAIVWNIENNKSIYRLNGHSQAVNGCSFGGGNWLMDMDKENQNINLIIATIGDDKNIIIYKNQKVLKKWQAHNDEGYCIEFSYDYEWLISSGKDKFIKLWSTRNFILLKQFEGHQRGIWTCGFSPYSQYIASASWDDTIRIWNVNNVNDIISVNNEFVNLPQVCINGFSRCVIASSKTVFNIWRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W9S6</accession>
    <name>A0A8S1W9S6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0790005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000079">
        <property type="protein sequence ID" value="CAD8182576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W9S6"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8182576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="121" mass="14241" checksum="20AB5681DB3939FF" modified="2022-10-12" version="1">MKLVFLQAEQKFKFMKSSSKRLCIEYEIQQNSFFFLEFRKKLKDLQSNLDKIPISNVSSNSKKQPPSPIKGNSRLLEAEDTQLIILDNIRNQKEKLLKAVEDELFIILNHNRLKQSKLESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WA34</accession>
    <name>A0A8S1WA34_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0850229</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WA34"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8185703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="424" mass="49631" checksum="A345CEEEC9DEF7CD" modified="2022-10-12" version="1">MKCLEFYNVNRIESDLVVKDLALNIKLIVVITQQIIQGVIKQNKQLILFRLHILWIILITLEEPEIRNSIWQIRRVNLILEHLKMNEQIIQFSQNIMLFSQRQSTQFEELESLMMKRTPQIPSRSRNLSSDRQQNNERSIKLSIENSRQRHQVQSYSVEKSLNLSSQNQKVENLIASWMQQKNLSSLNSSSNNVQPNNLLQNNIVSQFESQGRQSNRPHIIIEEDLLFTEEGDQQHAIQSNKVNQNKNKTPSTTRPHAKSIKEQQSFDVKKNAIERNVPKKNLNSYAQRPQTTKMHTSTQVKSSPIVYKEQSLHNPMKSSHIDSTKISNLNSGSNSYSNNINNRNKQNNQQKIVKKIQNSEFQLDNQCIVDLIRLKDQILSTYLKQNRALINSQNQIVKQYLRFLFRNFDEIVAILKQDFPQHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WAG5</accession>
    <name>A0A8S1WAG5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0860028</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000086">
        <property type="protein sequence ID" value="CAD8185853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WAG5"/>
    <dbReference type="OrthoDB" id="74705at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43336:SF3">
        <property type="entry name" value="GUANYLATE CYCLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43336">
        <property type="entry name" value="OXYGEN SENSOR HISTIDINE KINASE RESPONSE REGULATOR DEVS/DOSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GAF" evidence="2">
        <location>
            <begin position="280"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="GAF" evidence="2">
        <location>
            <begin position="446"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="34"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8185853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="590" mass="67633" checksum="AFDCAB8CACC62C29" modified="2022-10-12" version="1">MNKQQELNAFENGKKFKIKTERSTQIASPKNKDMDTQEDIVKKLTDNYKMQQKEIDKLFVTIENKEDQIRKLQDFVKLQQKEIDRLTHINEYLMQANQTKLSILRDERQLNQGGKTNQKFKTHKSLLPLEKKSNKITEEGNTQSVKLFRRQTFRNPTLVRFHQDDGDTFKNDRNTSGASNFYDILQQEDCFRTNAILNIQLSDEDATTQYNIDGTVQLMKVLLESEENFCDIIQSISAQKLSFLYDKFKRIMSDHQQLFILVLRLKKIVTGALQMNSSVLLDDALQTIIDKCIDCLECDRASCFIVDQAKKELWTRVAKGTQTTIRLQIGQGLAGFVAQNRIILNIEDAYRDQRFNTQQDVKNNYKTKTLLISPILDGDKCMGVLQCVNKSNGYFTKDDEALLQIMGEFSKSVLKNAMNHDAQMLIQNKLRHIIKTGIILQSKQDNLIDLILSAEERLRSLMNVDLAKVVYYNQVLSHINRDGKLSQTDTLLGIMGLCIKDQQLVAVSNCYTSPIFNPNIDIETNMPIICMPLKTQNHQIIGAIQVINVKGIGNISSNSEAKINSIDLDMLELFCQQCAQSLQQCKQSIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WC83</accession>
    <name>A0A8S1WC83_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">cAMP-dependent protein kinase regulatory subunit</fullName>
            <ecNumber evidence="3">3.2.1.143</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T0850186</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cAMP-dependent kinase regulatory chain family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the poly(ADP-ribose) glycohydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.143" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WC83"/>
    <dbReference type="OrthoDB" id="1937899at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004649">
        <property type="term" value="F:poly(ADP-ribose) glycohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990966">
        <property type="term" value="P:ATP generation from poly-ADP-D-ribose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009225">
        <property type="term" value="P:nucleotide-sugar metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006282">
        <property type="term" value="P:regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd22964">
        <property type="entry name" value="DD_CrRSP_unchar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000039">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000006">
        <property type="entry name" value="cAMP-dependent protein kinase type I-alpha regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046372">
        <property type="entry name" value="PARG_cat_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048362">
        <property type="entry name" value="PARG_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007724">
        <property type="entry name" value="Poly_GlycHdrlase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12837">
        <property type="entry name" value="POLY ADP-RIBOSE GLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12837:SF0">
        <property type="entry name" value="POLY(ADP-RIBOSE) GLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05028">
        <property type="entry name" value="PARG_cat_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20811">
        <property type="entry name" value="PARG_cat_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0114">cAMP</keyword>
    <keyword evidence="6" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="13">
        <location>
            <begin position="124"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="13">
        <location>
            <begin position="248"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="593"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="611"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="612"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="596"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="610"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="651"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR607724-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR607724-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8185619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="805" mass="94446" checksum="EA6CB839BBB5F50D" modified="2022-10-12" version="1">MSQKKATIQDYIKNKVKPVFDQLIARIVIEKPEDVYSWSINWLQKQQSGVQVQQLVHHDLSEEEEDEEEVVLEIKQQQKQQTRTAVSAEVYGEYNKKEDFKPRFIQKSEGQIERIKKRILNSFMFQALDEKDLNIVLGAMDEKKFQVGDVVIKQGDDGNELYVIDEGRLECYKKFTGLEEEKLLKTYIPGESFGELALLYNAPRAATIKAIEEVTTFALDRETFNNIVKFSAIKKREQMEEILSKIELLQSMDNYERVQLCDVLREEKHKAGETIITEGEIGDRIYLIIEGELEAYWKGQSEKVYDYKPGDYFGELALLKNTPRQATVIAKTDVVLLYCDFNSFRRLMGPLEEVLKRNVERYEKYLQNKYQLMIQMDINHKKLYKYPTLSAKQKFLLTKLISQQDQQSALSIIKDLIGQNEKQLRGLYTAISWLDKNYRDLFFQNTLPKMAQYSLDILELSPKKHLLISEGHIEFTRKEIYQFLSLAFFGLLEKQNDQFPNTYNLTYILQMEAEKAKCYLYYFIIANSELENEIVQLERINFNMNYHIHQFFPNPQANFLEDVKLWSNCEKSLQIIDFVDQKIEEQQNSILVDFANKYVGGGVLKSGCVQEEILFTIMPENIIAVLFCKVLEFNEVVIVKNTIRYCDYDGYGNTFRFVYRQPKNLNQNILAMDAQNYKNNPVKQYQQNEIIRELNKAFIGFSLSQFAKEEEFLQPISTGKWGCGVFQGNTQLKTIIQLIAFSASRKYENQQRRMIFSTFKDKQLQFIKRDVDNIIGKYKTVGKLFQQLMKITTKQGVFDFLLDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WCV2</accession>
    <name>A0A8S1WCV2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0870170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000087">
        <property type="protein sequence ID" value="CAD8186627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WCV2"/>
    <dbReference type="OrthoDB" id="547665at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24362:SF309">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24362">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8186627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="191" mass="22848" checksum="931DFCF6E581965F" modified="2022-10-12" version="1">MQTILFIKQLIINSHNIENQFGIMQSLSHSSNIINLIDYMILDTISENKHNENQQFAYLLMEKEEQKLEQYFNKRANCIKYWNNQQINLDTLTKEQRDIKFVNFLIMKHMMSNAVLKFKLFKPVQTESYLDTELLNFETHRLKYKPLKSKVQSLGLCFLYIVTIQLSQSRQLLSQSRNNIRLLKTLKNKYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WFV1</accession>
    <name>A0A8S1WFV1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0890116</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000089">
        <property type="protein sequence ID" value="CAD8187517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WFV1"/>
    <dbReference type="OrthoDB" id="294915at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="173"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8187517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="659" mass="78367" checksum="D1D4FB1EAD9DFB49" modified="2022-10-12" version="1">MYSKNLIDSDFQSNVINQELKPFVQKIQDRQKRIYELSKQLEENIQKSLSINSIIFERELRNKQKEMRKIKLENEKRVTKIKKEYDLSENSNLSKLLQKQQEYISNYEQLYQEEIHFIKFIDQQTKNSSYYTVIHSILNDSLIFSGQKLDNSSVLEYLTGQNKHKIPNFHSSIKQIIQQLQNIEENLQVILNSNNHDSQLLLLQQSLSNFINILKTTQTQIDELLLEMELIQKEIQDSDKIRKNNIILIQSIIKENQDQKELEIQEYIKMNDNLIKQLFSSIEINRKYSIFQQQSNEDLQKLSQLSEIQLQNKFQVENKKQNKQGFVQLKKVPTVLLSRTRIYSDHKKDLNDILNLSNDQSLEMAGRRDIMAKESSFLYQSQQTINTEKVIVNSVLSKQFSQIYTQSPNSTKYIEKKSHYIPHNTTYKTIAHSDKKTALPLKCACPPQCLCSAQTIMQIHQCNCQLYINSRGYIMCQQCQFSQQIQDYGFYCPQNRQKNKFKNYQDFFISFWSHLTTLTVNEQIVEFWENLQVNCQQQWIQYKQIDFNDLTDEVSFMNFIAYCPLNSKCYHKTSQQQIHFCQSPLIISSLGELVCKKCNFFGSLQFHFIQCPEQKEFYFYQNVDLFLNSIDLNHSNFESEKQNQEFLQKLRKNLIKKWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WG71</accession>
    <name>A0A8S1WG71_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0910077</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000091">
        <property type="protein sequence ID" value="CAD8188313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WG71"/>
    <dbReference type="OrthoDB" id="10249567at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000260">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24413">
        <property type="entry name" value="SPECKLE-TYPE POZ PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="115"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8188313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="315" mass="36845" checksum="EFB78F104E36443F" modified="2022-10-12" version="1">MLKKKGSEQLDIIFEAGEDLLNFSKNQVIMIKNNSIKLPIIKPRTFLSYSFEFCGITGLNYKMLRFQSLGRDIRPETSIMTSCLVKVIHTTEFSQMAQNPLKTSFQGLFILGSHSDILIQINKEDPIPLHKCILACRSPKFNGMFSSNMSESNQTLVKVEHSKPELFKVMLQWIYCGYWKEFPEEIEDTCDLMLLADEYLITDLKQKCEEDIISKLDSSNILYILLFVEKHSNIISQPLLEKTNSMFIDEFDKIHKLNPNLEQEITRMPGLMTKLFKNLHQKKIRKGRKVHFVVDDFEESDSDDYVRNYTQYFYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WGR4</accession>
    <name>A0A8S1WGR4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0840184</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8185136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WGR4"/>
    <dbReference type="OrthoDB" id="410307at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051252">
        <property type="term" value="P:regulation of RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000003">
        <property type="entry name" value="Zinc finger CCCH domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000018">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF18">
        <property type="entry name" value="PROTEIN TIS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="56"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="95"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="56"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="95"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8185136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="193" mass="23320" checksum="E21F89D6849FE95C" modified="2022-10-12" version="1">MSNSTPIEIYINLINDYQNASTDDDDEKFQIDVQPKRKYFETIEEKRQYIEEYTKKKKTELCKNFQLTGQCKFGDECSFAHGQLELQAKTHLHQKYKTKPCNRYFNQGFCPYGIRCQYLHDELKDQNRFEKFLQESYQQFNMKPTIARKYLNNTERLDIQRFQQVLKTFTKKGFNVGLHTRSKFFVQLQEKQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WN54</accession>
    <name>A0A8S1WN54_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0970033</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000097">
        <property type="protein sequence ID" value="CAD8190622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WN54"/>
    <dbReference type="OrthoDB" id="299634at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35381">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35381:SF1">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="81"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="325"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="163"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="220"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="325"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8190622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="566" mass="66131" checksum="3E890B8FCA9CA5AD" modified="2022-10-12" version="1">MSHSSMEQRNGQLLVMTIQIDDKRSGRIDVFEGDDPEILARDFCIKYNLNQRVAPLLIDNIQKQIQIAQKERINMVQYLSQKKQNQRPTEQSNEIQNQQQGSTPIIAQSQQSPMKQESMSQTVYDRLYQDAHNKKLKKQLQLSENKSSSQIMKSQESEVNYGLILYQKGIQKKEEKLQKAESARKDIQKSQLQECTHKPQINSLSSKIAQRSPKPVGEHLNELAKIIQEKRENAQNFKMEMEQQSCSFHPQINRVSSSIVDEKKKQSQIAIPHYESLYQDMDVKQQKLNELDRNYFAQKHTFHPKIDQISEQLVQGLSFEERRQKFRNKSKERNSSADEGNMFRPRTGRPPEQRPENLFDNLYKDAQLLAQKRAAVLSFSREKIMNQSKVRASQKSEFLTQQAIINSLANIFDQIDNDNDGEIDSIRINLGNLNQTVIQILQPLISEMEEGRHVLTKSEFIESAMRLVYTLSNKEKHDLLKKPNSKKSDIPIYTFNVHQSIQDHKKIQDQKIDESLQQFICLKLLNYINYNSLTIFFNYVVVLSINQQILTKYQKLLLLFLNSYYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WN91</accession>
    <name>A0A8S1WN91_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0950127</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000095">
        <property type="protein sequence ID" value="CAD8190091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WN91"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8190091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="63" mass="7586" checksum="1FE9937B24DCEA15" modified="2022-10-12" version="1">MSYQMNKAQSNNKFYIISKKKKNKYKIHTSIQTNFSELIDVIILTRPQMQQQGNSQKWFNLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WNJ3</accession>
    <name>A0A8S1WNJ3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">YTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0940206</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WNJ3"/>
    <dbReference type="OrthoDB" id="312247at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21134">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007275">
        <property type="entry name" value="YTH_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04146">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50882">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YTH" evidence="2">
        <location>
            <begin position="17"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="643"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="459"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="473"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="645"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="657"/>
            <end position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8189789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="666" mass="80840" checksum="36344DE749D2C05C" modified="2022-10-12" version="1">MEGKLNLYLDIFKQPSNKFFIIKYKTRGQADQSLNNKVIFVQKRFDLQLRNAAQVCEQVGVFLSCLDQKSFIAFGTIQNEPTDFDYLTEEQQQNLSKLQEFFQSFVKVEWMFKGVLNFEKTQNIKNDLNEMKPINHCKDVQELTYQTAEQLFSLLQKQKPYKPQPQQDIRIPHQLNKIFRENFGENNSDKQISSKSRSRLREKDQDNDKFKDQDDDKDKDRRKQSKDRKKEKKSYDHKSRDRRSKDRISKDRISKDKKQHSNEKQNYREDKSLNIQEFVIDDNKDLRDFEKQKERQAREQEQIKEKDQRERDRTKEQRDREKEKKQLKELEKREREEKEMIDKYKQQERERDKDKEREKKDREKDRERDRQKDTDYNRDREKLDKGRYNKKDDLKYRDRNDSKHDYDKRRKDKERDKNNRERSIDQSYEKSDRKDRDSKKDKERQRGGDTERERDYNYHKKQKDYRRETKRKRSEERLKPRSRSDERRQDHNQNKNKKALDIASLSELQRELYSSVSLQSHKNEQQQKDCELEVFSKLPKQKDEVDLGVILDVLDSPKQNDQQFQPLIQPVMITYNEQAVLTKQSELHQQNNHSDSKKRILVVKQEKSQSIPDIVDQKKSQQSGSMKIIKDAPVKIIQQINNQQSYQESKQGSGNSIKDRIILKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WQF2</accession>
    <name>A0A8S1WQF2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0960099</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000096">
        <property type="protein sequence ID" value="CAD8190399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WQF2"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001381">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="46"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="166"/>
            <end position="167"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="9">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8190399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="289" mass="34251" checksum="972BD2D84BF0E7E7" modified="2022-10-12" version="1">MFNSAQKRAPPQLYVTPRYRTTQSEYNNPHSERLFEKSKENSFNQLKILQQIGYGKFSKVMLAMLNGEKFALKIINKSCTIKTQTQQHLQREIQIMKQLNHPNIVKLFKDFSDEKNVYLLLEYCNQGSLFNQKFDQTQIKQIIKEVLLALKYLHNQGIMHRDIKPENIYLHNNHVKLGDFGIAQYIKKQSKSFCGTLDYMAPEVINQNFYDNRIDIWSVGILAYELSNLEPPIKDRDQKLQMRRIISEEVQYPRHFDHKLKNFISKCLKKKCDERATIDELLNDEFIHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WYF0</accession>
    <name>A0A8S1WYF0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1080017</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000108">
        <property type="protein sequence ID" value="CAD8194893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WYF0"/>
    <dbReference type="OrthoDB" id="1405469at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="19"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="319"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="410"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="665"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="814"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1006"/>
            <end position="1097"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8194893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1143" mass="137861" checksum="5D8F9DA66498B836" modified="2022-10-12" version="1">MNPNIQNESVIVFIEGKMYQRNQIFSCQGIQSINYIGTLNGNPNEQIIIKQYNSITNDETEVLRQIKREQNEIQPRCQHIIKILGIQDQAQSKLLVMEKGNGNILELIESNKIHSIVQKIKIFEQMVKGVIELHNLGYFHRDLKPENFVYFENENKDYKIKLIDFGLVIKDFTIPDTWVVGTLNYMAPEIMVTGMEYDKTADIWSLGIILYELLTSKIFLQAKNQKELQQQIQNLSQEQIYNKLNQVDDIEKEDKQLLNMILQKDSKNRIKLETLLSKINEYYNSIKQQEEKKLLEIEEIRQSVIYQLETFKSTIMIFIQNLNYKLEKMNDLNTEYQVKQQLQSYIDDQLKKCNEKIIKIEQRLQQIQSSQPKEILKEHYSEIIQESQQQIQDSNLIMENIIKQSFLLEYQILKEKEEQLKIQQEQLLKKEQEEGLKKEQAQFKSLFQIVRQLSSSQITQIKKISQEINFYQRIQQSNYGEEKILHMTTQHQTLTQNIQGLKQQDKIISQFDSIIEKITIYQQFNILLEEIYQDQNRLKISIEEANKFIEETDRQYLDEHYQQIYELSDQLKDLFDKFKYQSQHQKYKDKINEIIYDITKDFGQLEFLAQLVDQKTLAGYQEYKTKYIQIQQFLSNYQKQQQQLHQQIYNDQYFEQINEERLKKLIITQDKFDQFKNQMNQLKDQLKNLINNQNNNNEQTKNLINNELIRFDQKIFELQRQFDNFNELNIQNQNPCEIISIQIKLLEEFQKKLKEVIDNEIEKIDQFKLIIEKKAAEQKSEQEKEILYLNHQLQNRYRIFQKVLLSINFQFENLEFYQNLSAKYNQLLEQLQQEIKILQEYLSVQHENQRLFEVNLKDNQTQGGQNKQIIENLLKRLQQINLQKGIRLENIFHSVSKKTTDLDFNLVDENLIKNYQQLKKREEDLSIIFNTINQKISNKQLNPEQLQELKKTILELCSELEQFMIQIPLNDEIQKFKKVYQQNVESYEILYALFNYIKQYHLTRYYERIKENANQQKKIQQNQNQNDYKTLFQSKLHVDLEENTKKEKESQDTLQRYENILFKSYNKMTIEAMEKDKEQIEKQIEFIENQIKQKDLVKLRASIKDQSDIKEIIHNRQYYKITEFSQMLIINIIRKEIKQKNQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X0X4</accession>
    <name>A0A8S1X0X4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1070043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000107">
        <property type="protein sequence ID" value="CAD8194551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X0X4"/>
    <dbReference type="OrthoDB" id="5855668at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04047">
        <property type="entry name" value="C2B_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000309">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037768">
        <property type="entry name" value="C2B_Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045052">
        <property type="entry name" value="Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010734">
        <property type="entry name" value="Copine_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857:SF106">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857">
        <property type="entry name" value="COPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07002">
        <property type="entry name" value="Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="1">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="1">
        <location>
            <begin position="120"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8194551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="502" mass="58410" checksum="282C8F534726517A" modified="2022-10-12" version="1">MQQIYNPDERMNIELFLAARELPNLEFLSKSDPYLELYYSLQGEPEISLGKTETAECNLDPNWEKSFIIEYLPNLIQILRFQIFDENRMGREFMGEAQITIKDILQSKNSLISLPIKRFDKNAGQLLCKAVQMKQSNHFVQWQFSGSNIKNMDGIFGKSDPFLKFYLYSDNVWCQIHQTEFIKDDLNPTWKEFEISLQRLCLNDENKKFKIECLDRHEKGKNNQLIGSFETTIDEIFNKNKEEFSLVQPKGGQVGTIRIKNKKRIYRANFDDYIKQGTTFNLIIGIDYSTHNGVPSFPDSLHSYIDNNNKYGKVISDVAQVLEKYNQKKLFAIYGIGAEPHLTNYTLNTYQTLFPLTGDFQKPQVEGYQEVANLYKNTLKQIVVKGDFQIEDYINYIQTIAEANAGKLIYTICVIIAQGKITDAQIFQDLLIKGSKLPYSLIFVGMGKNDFTEVKRIIDKINNQQPPIRNNFTFYEYKEQTIQMLSKHLLNDIPQHLVSYKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X4A4</accession>
    <name>A0A8S1X4A4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1090084</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000109">
        <property type="protein sequence ID" value="CAD8195369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X4A4"/>
    <dbReference type="OrthoDB" id="307856at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8195369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="382" mass="46267" checksum="5C1FD4935238149F" modified="2022-10-12" version="1">MILSKKNKMKQKQVKINYIKNFQHKHQLHLIPRILLHNKFKCFTSSTSPLQKYHIKQKDSISTVSHFLIENKQMQMQSQSEQKEVIDRLLKWGKRKQSEIQEQQQKYQIEQKKNLWDKPKLSKETLKIFESGLKHHQELNNFIKLKHQFTQLSNNYPPLQKLDKLKKGIKIKIEIDKEIEDNPDDINDKTTQYKCINEIQSNTQGILNREFRSISDYTTMPIHLRYEYEIKLKNAKLKQKIDEKEKAFQKELELAKKYNSTTEQFEHFLKEQENFLKKKQQFYDTNLKKELEKIQEIDSQFSYKPDINLPPQSIISTNNQQQDDNFNEKLHSLRNRQQRQKNIYFFKSTLPDLRRNIIQKYYEILHKSDYMTISHIQFLTES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X6D9</accession>
    <name>A0A8S1X6D9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1140011</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000114">
        <property type="protein sequence ID" value="CAD8196897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X6D9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8196897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="310" mass="37129" checksum="268EBBD54275024E" modified="2022-10-12" version="1">MNNHLKTVLQKIKKQRMDLSNQIQIAIKGNFKDSFPIRGQLQNSQVKNESNLSSLYSQSFEQESLTASKKVVQEPKQPLPLAVKPILNKEKVMILVWVNLPSIYNEQSKEFWSKKQRGREGSYVDKSPFRYVSQKLKFWNMIFDFSSYYQMKLKKIEIKRCIKILQNKFLINGKVQERKEKQIITKFQQIIKKNQEKLQDILFQLSLNQQFKNKCMIRMCSKLCNLQKNNYLINFKNNKMKYIFKFLIRVLNQISQMQQIMLSLLSLRQKKIENQQMLLVLPIVKEDDLQMSVQQIKNRLMFVSMRKWIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XCW4</accession>
    <name>A0A8S1XCW4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T1190103</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000119">
        <property type="protein sequence ID" value="CAD8198642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XCW4"/>
    <dbReference type="OrthoDB" id="421009at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15844">
        <property type="entry name" value="SNARE_syntaxin5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF3">
        <property type="entry name" value="SYNTAXIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="9">
        <location>
            <begin position="178"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="117"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8198642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="270" mass="31815" checksum="663A95038BBA0DC4" modified="2022-10-12" version="1">MDIASSQYTDITQKFLSKVHIKLVAIKREQPNQFLLTAKLAKEKVDTANERLQEFHTTSQSTGLFNDQDYKLNTLLSQVKDDISQIHIHLNQLKTQLNNDLHQSIFDFVQQKALKTSDQFKKLLQSHTQRIKQQEEKRNRLNGERDRVIKRVGFNQKYQKLNETEEETNHQTIQMFEQKQNEEKLVSMQKIESMLNDIAGVFQRVGTMVRLQETMIERIDKYTDEAQVNVSKGRKELQESHKRISSNRGLILKVFLILFIFAFIYIVFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XE24</accession>
    <name>A0A8S1XE24_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1210018</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000121">
        <property type="protein sequence ID" value="CAD8199133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XE24"/>
    <dbReference type="OrthoDB" id="299842at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8199133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="184" mass="20970" checksum="CAF323F3DF6831D0" modified="2022-10-12" version="1">MATADIIIAHGMPSKKEVISSKRIYPEQHNRKGAIQLTWDVQGVFPKIAQKIGVQPIADDPPLPPKPKYIPPPLPQNYVHPPIKKQGKKTGKPLIVAEKQSQEESIAKKTIRQSRQSLSNTNYGKEYGGFYSLDRCFLKRDKEFIKKEVKGYGNFYEKSPEGRFMHVPQPPLSYKYQYVASTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XLN0</accession>
    <name>A0A8S1XLN0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1300180</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000130">
        <property type="protein sequence ID" value="CAD8202330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XLN0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8202330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="386" mass="44367" checksum="7213979DCA4278C1" modified="2022-10-12" version="1">MLLLQNVGHKQIVLDSTRKMQTDNNCYYLNFSIQKNKQVMKILQQQLILNVILIQVDVLLVVKEVFQIQNHAHHTENTTYEQSSKDAGDTATSKCKVRSSVETLQLQPMLNVHSSEMLQKQQYWMCWLNIGHSLHLREIKQLVQIQGIFNTSDCVKKKCRNISGKHKKECNDGKPQFITINDPLCVFDGTSCIYYGKLCNVSMELNRQGNIFVQNCPSKQRNMQASEKLLDIWLWIQKRLYAIQEMNDIGQANVQQLVDHVLLYILRITLDVKSKSLSTIATVNSHAQHQHLAGNCTISSARCITISICSSYKTQLFVADLTHKNGVARCVWDATINKCKDKVCSDTKMAQLMVNIILSFRMYNKWIRLFGFEPKVLNLPIDNSQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XLR0</accession>
    <name>A0A8S1XLR0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1270125</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000127">
        <property type="protein sequence ID" value="CAD8201392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XLR0"/>
    <dbReference type="OrthoDB" id="687730at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8201392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="199" mass="23918" checksum="EF604010E6EACC20" modified="2022-10-12" version="1">MAIEQEQQPLNAQLRQTENKGKLIQNATKMAFQQLKDRQYRKNATSKLQDLQDDSSFSKVKYQINQVEEVFTLHIFKFDKEKNSKMRTSTSNFVDEIIFLHSKFNMNNDYKYLGLQNKYNGSMKANSNNFMKLRKLILNSQKILQISFLNQIQRTLNDKLKSKQIVLYRQYLREIFKNQEQIYNFWIQNQKQTNFLMDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XN79</accession>
    <name>A0A8S1XN79_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1300068</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000130">
        <property type="protein sequence ID" value="CAD8202154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="285236at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026306">
        <property type="entry name" value="RSBN1/Dpy-2/CEP530"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13354:SF11">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13354">
        <property type="entry name" value="ROUND SPERMATID BASIC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035724516" evidence="2">
        <location>
            <begin position="23"/>
            <end position="1814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="444"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="319"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="380"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="456"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="470"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8202154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1814" mass="209888" checksum="C027F98C946136E3" modified="2022-10-12" version="1">MLKQLLVAWLLVSLSLQFKTSARTQITPLESILSIDPSGWTCESPSYKEQLGAQHDIDQWIDIVKHKDGFDGDLQVLAMVKALVDRVLLTVQHIQGGSFIQVKQLHHQISKHFKSLSQIESTSSWYDINDILGYLEVLNKAKSEEDKLQLAQTIATKISELIQQITEIQLQLNTIPIEIDQLYDLATRVNEKTSQCKDYWAPQLEADARAEEEEKRKEQEQEEARLRAEEEAKAAAEAAAEEERRKEEEARELEELKNRVDITPEEAELLEQEAEKELELAEELEAEAEQELDAAKQAEQDAETQAERDAREAQEAAEKAAASQQALEDAQNKEEEACLDAEEAQRRLKEAQEAAEEARKRAEEAERLAEEAKKAKECEPVPEPESEAEEEEEEEEFEWESEEEDGCGDDVLEDLIDTLIDAATGPSAYQPSCGYPGCQEQPIIIIVEDDEDEPKPVPQPEPQPQPEPQPEPEPEEEEVVVEEQPEEEIIEEPTPDDEKVEEQEEIVEEIVEEIVEEIVEEIYEEFVEEEDDDFGRLEFPDEPELEQLVYTLDEKTDSVVTQETSPWEKEVEDGPESRIKKSVEYSYGLWTYFRYNGKVKISEKKEVLAVGGLSGDDTNRLVTTIGEGFYNFQVFEGEVVTELQLDYGKYLDAEWIYVYFGYKEGKANGYIYWGRTQVIKTVTFTVTQPAIVRTIKFQSGIWKGFKGFNGVITNVRVVLNKNSFVETEEEMKTLIETKYTVPTDPDLEYKEVQQYDKVEYDGIITDTKYHWDLDGYREYSVSTWFRYVRRTERAHQIIFRFTSNEPDSGVVAHGLKVLSLWQTENNEYQFSTYTVEEKDQFSDIDETVTIAQESQQLWTYAYFGYNHGDFQYYMEWPDSKVEKQFKGWHVVAKYWALYLGQGLKYFFHGKTAYTTIRFGKGAWGVATDFDDYKIGKVKLWPGVQLKTYDHKWMNHKTESEYDTLLHEEPDWVTDGVYEYGFGMWTKFFMTNPSRIYEKPDRILVGRLGFTDQDEAEFAVYIGRGNYEFWANSGRPVNYGKNLDGYWNYIYFSYSKLKQIAVGYVNFNKLKQVQRTVFDPFDADPANNYVRFYVGLWKTSVYGFNGRIAHAVVRFGRGSFINEIDEYETWIEQYKVPVDEELGTKDYEIQGADNKDTETYDTFVYEDGAIETEQYSVYGWFKYYGSLDDTEAQTVIRLTNNEPGFLKDADLLGDRTLSVIQKGGDMEIGTYDIGFIDTDFNLNRNTNIALGEYRGIWMYIWVGYSRQDEYAGWFFGFPDISKGGLLKKVLHFSPKYLAVYFGKDGVNKNFVGKSRHVHACYGSTQCWHYVDKVEVEVDLPAWIPYKLNNYFEFYVQNDADALIVAQGDDPALDVEFTETNFPGSDIEAIYEYGIGLWTRWLMNYPFILLEKAESHSIFRFTTNAQYEDAQKNGDRTVSAFVGRGEYRFSTYDAVLDKNEITTGTKFDKELEGYWNFVYFCYKRIPTGPKGIGYVYLTHQNVVKRVEIDSAKHWLLRDYARLVIGKKEFGHSAFQGKLFDPRAFLGKNSYIDSSEDLLNIVVPKFRPYPPYKDKQDNEPVQVEKAKMTQRVFKSYEEKYSGVFEYSVYGFAKGNKLKNVTDWTSLVRVTQNTPDIQADNDNAGDRTLSIFIDKGVWYFSTYDYGDIETIDDDVGSIDKKFDIGKYWGKWTYFYFGYSFHLKQAYAYIRQIDQTSNSYLFEEIYHFVPNYLSVFFSEDGYGKQFDGEAFDWYLGIGDGAFTTNPEPREWPVDPAPPPDRILSALLANQGFNSGRIIKGGGTFLQVEGSVNLIQEDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XNC5</accession>
    <name>A0A8S1XNC5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1310052</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000131">
        <property type="protein sequence ID" value="CAD8202467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XNC5"/>
    <dbReference type="OrthoDB" id="303271at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8202467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="209" mass="24791" checksum="8D1F17933B048689" modified="2022-10-12" version="1">MNQTKIFETRKRKSQSESEIKNICFITFEYMPIEKYPNKKSQSQEQLLEYSSDEDSLDDQQGQLDCQINSNTLINYQQVPSLVSIYTSESNISAEEEFEIWDDNSDLRVLKIILEDQQPSIRQFLQLYKARFGGFPGQQQCIQNEEVIKTAINYASTSKQLFKKYFPTKRIVFEDDIDYSEPYKIEEDHHFQTFNDFFKGKRAKLLMDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XQT4</accession>
    <name>A0A8S1XQT4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1340005</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000134">
        <property type="protein sequence ID" value="CAD8203450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XQT4"/>
    <dbReference type="OrthoDB" id="2443807at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050349">
        <property type="entry name" value="WD_LIS1/nudF_dynein_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44129">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8203450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="376" mass="44465" checksum="57167F826ABF1D63" modified="2022-10-12" version="1">MEEYLGIIKHAKIKVLKQKHENELDQVHIQVKTQDYDPISGNRKTNSQGEFITFLIKDLYDNYDGKVNEFLWSDQGKKKDLILIKGKAGSGKSRASHYTISPNWIQIYVSLPSLKDPHHNLIDQALKSENYNIDNIQIREFKDAVINVKLKIVLILESYDEMKFDCIALSNQQNGLRSQSLSIRINSKRDYYYKRRNLNSIGYQTWFYGQNIDTLKEIEILPFSYEQSSQYIKQYVEISVKRTIKKFYQFLKQLKGQNFSLEQFKLIWNQLENIINSMVLLQQNSEVLFSSQDVDTQNKFKQYSLLLLQNQIRWYLQKRNFFNCGESKNSLSNQQCEYKSFIDNSIYDGIIVYVLPNISQFFSKPNLIRDLLKRII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XW63</accession>
    <name>A0A8S1XW63_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1400138</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000140">
        <property type="protein sequence ID" value="CAD8205410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XW63"/>
    <dbReference type="OrthoDB" id="301055at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8205410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="483" mass="57128" checksum="6D23B03FA5E45E01" modified="2022-10-12" version="1">MRKKMQKMHRHKLLYEDVLKQVEKEDVAFFSKCTKFRDQPPQPNTLDRALQFLWLKEKKIDRKVALQFGQRRVCKIDSTLKHDGPSSARIIKTIKTDQGMINGWYNGSGDSKFDLGPQYNTKQLEKNNEEIEYEELIKQDQQSPSEYKIKLNHQDIYNNEISEKYSFSNLETKQLLQKLNELKQINKQRNEQPQEYVELEGQRFYHVLNDLQDYLDKKIDNKTLIQEMRAFQTFYPIKRKLQVKQLNPGDSLSQGIESQIDGTQKATFRLFNRTRTIANMKCDLFNTNNYVFNVKEVPNIGQQLRRNHFSQLIEELRENGMKTPREINEVSLENSIKGSQLESSENDVKQIRKFYLKFPKQKGELSQTTSCTKSQRIGTLQSQISNQYLQQPLKTFISNIHGSSTEIKLENPGKFKNRLDSRINKYVKQYIKNQNVIQQNQNVDMTILKEKYKSLKTEREKEITRMYKSQQIEGINNKTVHLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y0W8</accession>
    <name>A0A8S1Y0W8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1470069</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000147">
        <property type="protein sequence ID" value="CAD8207403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y0W8"/>
    <dbReference type="OrthoDB" id="313601at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8207403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="435" mass="52375" checksum="2D450298814F2697" modified="2022-10-12" version="1">MQNKEILHKVFDFYCKQQYVQGQFASFERQQYEASIFTIGKWMHFCRDFQVKCNSARLLELFKKQAKHSKELDFDQFIQLLNVLAVEQEVDFGQYQSNLGLDNWKVCVSKMKTFQRPFQMKDQYERIKDIKYQFKIYHPEVKNDEQIKQILLQRKQQNEQLKLIERKKKAFYKLQFELKHFTKSQLLSKYPTLQELIEKLPDPSKSKIQSNSNKEQDQNSNISQQMQSAITWENLNYLPLAKDFLTDLMEQEQEDDDDQYLKEYQEQKQVENSPKKLISNNRYNSIQDYQNEREQRSFSDHKQIIKGKRYEELYPIERQLDKSKIFKLKTTSNHSESNNIISLQQIDSNLIRQQILRQQKQQLQNQNYSVELPRQNLSQEYSDIPFLKEKPTTNKLNISMLKRVQQLSGLETIKEQNLLNKIISQQREKTKIAQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y1C2</accession>
    <name>A0A8S1Y1C2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1480137</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000148">
        <property type="protein sequence ID" value="CAD8207821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y1C2"/>
    <dbReference type="OrthoDB" id="286133at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47823:SF9">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_10, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47823">
        <property type="entry name" value="ION_TRANS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="33"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8207821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="308" mass="36152" checksum="8A30A28ED7934A0D" modified="2022-10-12" version="1">MRLQRNHQGAIQILKNKIESYEHLQKQQILCQLFPDARIKWIKLHLDKVILTTYHLNQMLFSQNQIPTHVYYIIEGEVRLEETQIERRKPKNNMFASTCMAKNRTLLIKSAGSLVGEIEALLEKPYQTTAKSNVHYSKILLIPEAIYIDLKKQDYLHQSLETQHQKDQLILKKIIQLKRRRLELQDVSPDRDSPSQTKISREDFIVQQNKRIQKKQNFEFINLTPEAPLFSVKKLKRVAAPDEKLENRTRSISAPTKAKMLSTIKSINSTEKILKTEEITRSRANTINLGEKCRPGSRIFWQQIFQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y2R2</accession>
    <name>A0A8S1Y2R2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1450136</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000145">
        <property type="protein sequence ID" value="CAD8206948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y2R2"/>
    <dbReference type="OrthoDB" id="303154at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="271"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8206948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="348" mass="41123" checksum="AB0DE78F4DE111EF" modified="2022-10-12" version="1">MSEQQTQKSIYPFYQFCLGGASQNFLKCTNCFILGVIIIILLFILILQLRRRYRTGTKKLSKKCIILYMIITNFLIESFIISNKYITQLEPYFRTMVFIFFYYYVSLKLSKLSTDRKKAQAKFRYITLFLFALVLIGLIFVLVELIQDDQVFYECYFKYSKISRIVGGCVTAILVIFSMKLQNKMDDKVQPLYKDLPLLQMKTFQNNETQREDLRILIVMYSASQFLTIFQMIYFIAKEQLNSELQFPNCTPCQMIPCLKMSINNTIAFELLLRLCFLTIIIILPYLALISFFWVNSNQLHNDYHRTISNEEDIWINFFKKMGYEKSKMALSDITEQQEKSSSLDVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y6Y5</accession>
    <name>A0A8S1Y6Y5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1530041</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000153">
        <property type="protein sequence ID" value="CAD8209053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y6Y5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8209053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="117" mass="14118" checksum="4F93CC66B2A0B9E4" modified="2022-10-12" version="1">MEQLFSRRFCLFGKLQYNLSSINSTFYQNLQEYLLNSFLPNFIYIVVRISQYYYSFEKDTSQPEQRNDGDTLKQTLSNLIAIHMEVNSPSFMGVKVLNKFFTHIQWHYYNKLEFIQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YA83</accession>
    <name>A0A8S1YA83_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1580113</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000158">
        <property type="protein sequence ID" value="CAD8210313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YA83"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8210313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="115" mass="14103" checksum="38FD114FC7233014" modified="2022-10-12" version="1">MIRILIKFQFFNQRMEYSNRIKTKLFNQIQINYVKMIGDSSKLYIIKIKICYQLDINIIFILLAKQMKVTLRQEDHQIVKLKIFLELLQIMHNIQYFGITNKRNIHHMSYYKNET</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YBF4</accession>
    <name>A0A8S1YBF4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1630066</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000163">
        <property type="protein sequence ID" value="CAD8211405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YBF4"/>
    <dbReference type="OrthoDB" id="286188at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8211405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="328" mass="39194" checksum="52459F53D88FBE62" modified="2022-10-12" version="1">MFNSYQYEKQLTFTKDIDKIKKGYLREREVRKELNNRLAQNYKKRVENFVINMLENPIVCVDYHQPNQSAFRDEDPSKNLGDPQFYVKGFKHEKDRIQEALDKNKDLDFLPNRQVAHYCFRERDPSKDIKRDIFRYRDKTSLERIEQFLKDHTQTQVENQKFPKKKLINVETLSDNMTSQDRKAYISRLVAKNLLPSLHQKTHFQAASTMYNNLPLSLMDHTRSLPQMKQLDTEEKKSQQQQREVQKTEENETKDNKAKQNNEMETFNPIETSKVILERCQVIRNKNPKTHVIHKGEGHLISTLDKSIQEVYKEIYGIDFSEPKFFQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YF81</accession>
    <name>A0A8S1YF81_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1680077</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000168">
        <property type="protein sequence ID" value="CAD8212500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YF81"/>
    <dbReference type="OrthoDB" id="287354at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8212500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="224" mass="27064" checksum="316820782BCC47C1" modified="2022-10-12" version="1">MILDIDQEIISQRRNKLRILLNKPNHTKNLCQQQHQIIKSVLEPPKRIFSYSRSIRHFQEIMSKTGSVSVNQKAITLYFKQREANNQKLQSDQIKPTNSKFIQTDDLITEEQNSKTDRTLWNMQESPYIRKRKFANHSYQKEKYPKQLQLHKETQEYHLSNSPNTGRNIKQYTFQNEILKQKFDSIIPFYDRSTHFFNKKRLHQIHHDKCEKLDTIFTTITHRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-02-22" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YJH8</accession>
    <name>A0A8S1YJH8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T2120003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000212">
        <property type="protein sequence ID" value="CAD8214415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8214415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="113" mass="13563" checksum="C7E09B0F9065B83A" modified="2022-10-12" version="1" fragment="single">MIQQINLKFKCQQDDHKDEIDTVCYNQFCTEFRLNCFKCIKKGKIHNDHFDDVEKINTIMDFIENKNIECDNLINDLNKLVESMNQSFSQLKMGIRFKYSLQKERLEHLNSXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RWY5</accession>
    <name>A0A8S1RWY5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0010021</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RWY5"/>
    <dbReference type="OrthoDB" id="294358at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="230"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8131425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="542" mass="66009" checksum="EF129B8ADBC6F9CD" modified="2022-10-12" version="1">MNQNYEKYDENFQMIQKLEERKIKQIFSDNLTLSTPTFMSSQRKQTDIQYIHKERAILDVRLTNVIQLDLCQFHYIEVPLFGHKKPLVIHFQFTDQAKMKFYISSHTLTPNRFNNEESFERKQILRYTEQGENLSFTQRCLYLAIFSQQATTIYAKASFGIKPQQQQKNDIESKLRPSTGYQFTRSSQFFFTRKPSKDMISLNKNIEQYQPDKIRLQQNLRRVQSARKISETLHKKKQIFEEKKETLKERFEFQEKKKILKEIILYRNNIDSFIKFQQKLWVQNLYFIQMISIIKKKYYDNIIFQFKQSIKKRRQYRVRQVLQRMLQKSGKDIYQRTLFEGLLILRMLSKVKENGAKKKSKRIVFTFMLSYGQIGEIFQKTYQFKRRMRKIIEVFRNYRKKVQAYANRIIMLWNKYWNQIYMEIKKEDQEKVNEIKVQMQKTIINYHIEEEIIKSPYLDIKVQSVIVNNYYKEIKNLFIRKWRVLKIKVKSSKFQTLMASQAFDKKNELLLFTIVDKSVLKQLIYKYMQEKGMIQSLLLKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S109</accession>
    <name>A0A8S1S109_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0030019</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8133876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S109"/>
    <dbReference type="OrthoDB" id="287335at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8133876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="232" mass="27779" checksum="A97F489611A9784C" modified="2022-10-12" version="1">MNQAQLFDIFFPQYQMQQEKLNRKLYILPKVESCVKLDIDFRLSTPMHSMANKHYQGCYPKTTRSVRSDELMNTPTMTTITISSGKRIKLYAPMQESKRAQRLKNVPYELQYFQTKLPTKNFKSSHLMSEDLLNQQSESIDNKQKWQMRSHINKMNKATTSYQIKRQERNVVKQTKSTDDLTYIKQQNEETVENEKLKRYHLYKRSTQNLIRQFSDTHNIKQLCCDLQFKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S238</accession>
    <name>A0A8S1S238_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0020524</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S238"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000097">
        <property type="entry name" value="Probable serine/threonine-protein kinase nek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000957">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="8"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="316"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="467"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="467"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="482"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8133643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="520" mass="60552" checksum="AE1ABF2604582BDB" modified="2022-10-12" version="1">MESKLQNFEILNKLGSGAYSSVYKVQRKSDGNIYALKKVKLVDIGDREKQNALNEVRFIASIHHENVVSYKECFIEDNNLCIIMEYAEGGDLLQKIQRFVKKQQMIPEQEIWQVAIQVLQGLRALHHKKILHRDLKCANIFLYENDQVKLGDFNVSKLAKNGLVYTQTGTPYYASPEVWQDKPYDHKADIWSLGCVIYEACALKPPFRAKDMDGLYKSVLRGQYQPIPVIYSQELVQLIKTMMQVHPQNRPDCDKLLQYQYVQKKAKQYGIPLISEDIEDDLLKTIKWPITRKGLQANKSELINLNFQLPGSNYLNQHNSNHIRRNQQSNRIKSQDTNDSISLNQPNEALHQLMKQITQLEQKDIEKSPNVKQNLNKSTSKQTIQDNILPPDRRLRVSNSTHEKHEKYESVQPKCISKDRIEHQIQINSNYYAKDVIQSLNKQHYSQKLPIISGTQRQDEEQYVLRKNSSNERNPSNHIKRSKNDSLKKDLSMQPSAERPTVLMKIIEEHQQLPKIKKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S248</accession>
    <name>A0A8S1S248_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0040215</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S248"/>
    <dbReference type="OrthoDB" id="312082at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000616">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="11"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="305"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="338"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="35"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="313"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="345"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="371"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8135421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="503" mass="58064" checksum="AB25B25514FA0668" modified="2022-10-12" version="1">MHSLSILNGRYVAQKKLSSGSFGVVLLGRDKENNIDVAIKIEKEENQEINSLEREVQVLQRLANTEGVPNLYWHGEQDDYNIIIIQLLGKDLAYYMKQKRRFSVKTAIQLGIQIVKVLERIHQKGVVHRDLKPENILFGLDNESSKIYVVDFGISKIYLDKIGRIIPFRDGTSFIGTTRYASIAAHKGYELSRKDDLESLMYVLLYFIKGQLPWQNMQNVTDEERTTKVGDMKMTIDPRELCKDVPIEFPIILEYLKTLQYRSEPDYNYIYNLFQKSADGLGIILDYTYDWEIPSQRKISANEMVKSQEMKRSTTLNQALLPPNQQEMKKSLEKGNTNLMRQSSNNNFLTPPQNNQLQPPLLRRQDSQQPSVASNQGSAMLSTYNSRRPKYEKSQVANLDQFEDAKKSIDSIQLENGNIDKLQTDEQKFNKILNESLQKPQISNIQQSHYCSETVGWKDSIFEENYLDDPLSKKYGLLKNNGVDIGKKLSLQNNKIKLQKKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S4C7</accession>
    <name>A0A8S1S4C7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0030148</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S4C7"/>
    <dbReference type="OrthoDB" id="305874at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000097">
        <property type="entry name" value="Potassium voltage-gated channel subfamily G member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF254">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN SHAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="223"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="12">
        <location>
            <begin position="56"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8134130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="389" mass="45242" checksum="9C602F94CE51546E" modified="2022-10-12" version="1">MNQVSDDQSYKTIKSGKSMAQIGQTVSGEYSSEKKKQIQQKIYIILNDYECNKLAFFLSILSIFIISLDIILLILETEKELGIIDIWVTANSGVSSYFLVEYVFRLISYNAFDETLVEFLKKPFNLVDILALIYSIIEQILYLIDVQVDPRIKVLRMLIVLRLIRIFKFSTFSLGVKILIRGLSESMQALSLLLFFTMICVILISSFMYFAEKEFVEDSQIQNIPQAIWWAIITITTVGYGDYVPKSLLGKFIGVLSLIFGVLLLSLPVAIIGNKFQEIYLQNKKEETKRARKNAKTHYNQIQNQNEKEIYRIILKLNELEQVNERIEQCLKDNQFLYRSISRDAQSMIDKIEINRENGKSKSKQKERLSTQERIIKIREEILNSRKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S6G3</accession>
    <name>A0A8S1S6G3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0040397</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S6G3"/>
    <dbReference type="OrthoDB" id="298650at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8135738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="461" mass="54105" checksum="51BE84905A1B6640" modified="2022-10-12" version="1">MNQEISKEILALSIHVAAAASSITIQTQQRMTLVTNSVLETVSQTMKILNCLMMCSQKQSHLEAVTEEQEDECQKDSKTKECLVEQFQKTIQTDCCNVEVMENQKSKENSNKNMNMKSSSELREQSILKSHNILSEEYPKCYVNAIENSTPKQIRKPSLLQQQLSQIKVDKQKEAEEQRELLKLIESVDPEELIPKLKSIEVYDEQKQSSSHKDVLVFSDKQQIVINEQDEEQQLMDSPNKSEWYESSSVEYRATSYNTNESSNLDHHSYKWRSSSFRDDNIIQECGFQFQSSVFDFPFPEDFCANSQNIHTNIQNIENKNQHTPKKKRLYCNNKKVPHWAENLEKVSQHQFQQQNLSQNQIFGRMKQRVLDIAKQFSQNRFNRRGSSAHWHISNEKYEQMQKQMMKLQQIQDENIVKLKQHQQDSVKKNQGIYAKTQYFLTSIKKKILNSFEKESKYKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SCL5</accession>
    <name>A0A8S1SCL5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0060217</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SCL5"/>
    <dbReference type="OrthoDB" id="10251809at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14733">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093:SF18">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="383"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8137688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="541" mass="64094" checksum="0BDEFE783C2A87A9" modified="2022-10-12" version="1">MNRRLSSEQDNLRTKRALMRQQINQSHRVDREFEIDERFNSVSMFIKHDEFEIDECEYYDHQTWERLDRFDQVLPNQRNNCSWVIYEDFLYIFGGFTFNGRLDDVHRYSFSSNQWQHLNTTGQKPSARENNGAIQYKDHMYVFGGCDGLLWLNDFYSLNLKTLLWKKIEPTGQCPSERFGIACGAYQTKMLIFGGCDGNHYLNDAYVWDFEEQVWNKLQLIGDVPSARSCPSYSTLNNYIYIFGGFDGVNRLNDFYKLNIFSGKVKRISQHGTIPCPRYFHTSEVYQNKLLLFGGFNGQARLNDLYEFEFGSKTWKKLEIHEPPKGRSSMVFQLYNDSLYVFGGYDGDELLSDIYKLEFKNAQVPRSSFIQDLRSLINNPLMSDVVFIVEDHQVYANRCILGARSEHFQTLFFEEFRDKEQIYIEITECCHQTFMEMLQYIYTDQLDSSLSTTRLLSLIVLSDQYLMQRLKYLCEEQLIRKINCNNVIELILFSKKYNCRLLRIQTMKQLVDNITTIKRRKDFIKLAQEPEILLEIIQKKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SCW6</accession>
    <name>A0A8S1SCW6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">SPX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0060299</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SYG1 (TC 2.A.94) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SCW6"/>
    <dbReference type="OrthoDB" id="9970435at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000822">
        <property type="term" value="F:inositol hexakisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016036">
        <property type="term" value="P:cellular response to phosphate starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004342">
        <property type="entry name" value="EXS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783:SF103">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 53 MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783">
        <property type="entry name" value="XENOTROPIC AND POLYTROPIC RETROVIRUS RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03124">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51380">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="260"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="316"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="345"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="502"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="548"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="8">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="EXS" evidence="7">
        <location>
            <begin position="436"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8137798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="708" mass="85056" checksum="E30EFB6B592A4F76" modified="2022-10-12" version="1">MNFFDALPSYLEKINQKQYRQEMVPEWLRLYLDYETLEHLMIVGAELRHKKNSLRDVNPIKYQELVQNQEIKNKIEKDKSSFLLVLEQELQKIESFILYKYQDLLCKLQQLQEQIKLMQLQDKLHKSKIECLKQQFYELYKQELQFQSFILLHIKIMQQLQYEIQYCFKIDILIKDRAILEQLNYKLDDDKSKIHQLLSFNFYLYDPDTCRKNLQKYSIKKKTGSKNIYLFGLFFGISIVIVTILLLMRLEGMLDPENEALFSPIFPSIRGGGLLLIYYWLLTLDQYIWIKYQINYKLYLGFNHHFSTLTEVIKRVSFLSTIFLLLFLITCIQVEEIAFKDYRHVIKILPLLFWIIFFGYLLIPTIKKFNGQGRRWMYRMLKGALFTHFISYDARYTFVLDQFVSLFSPIRDLEYTVCYYSIDLFNDNKEIIHYKECEQGQRIVADICLAVVFSLKCLHCLTLAKKNGKFINTLEMWNFLKNLLAVSVGIVGCLNKFDKSDAILWIILACTFTILQQYWEIKNDWLFLQPDSKYKFLRSDLAFINPHFYYFLIILNMIVISAWAFTISPQMYLYLKVPNQQLFILIVGIMELTRRFIHNLIKVEKEHVLNLRRFRSSKDLVYPFEQKGEITAKIRQSNAYNVSVLEFQRLSQEFTRNTQMNLNKQEIEMLLQQPYDQQIMKMNSSIDRKNIRQQLQTEYYIKLINQHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJ09</accession>
    <name>A0A8S1SJ09_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0080344</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJ09"/>
    <dbReference type="OrthoDB" id="308342at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019351">
        <property type="entry name" value="DUF2039"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22876:SF5">
        <property type="entry name" value="CHROMOSOME 9 OPEN READING FRAME 85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22876">
        <property type="entry name" value="ZGC:101016"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10217">
        <property type="entry name" value="DUF2039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8139888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="286" mass="34171" checksum="F9A347151FDBD8D3" modified="2022-10-12" version="1">MKKGAPKYQNKIAFKMRFHDHYTEKVQNASLDRLCELCLAQIEWKIKYGKYKTPHDLSRCYKCEKKNIFKAYRRLCDACSDELKQCSKCTEPIEINNDSSSGEEMDETLKCLRERSKRTVLRKIEKGEITWDKHNKKFIDCETGEQMIVQYKKDYIDEEDEQLWEDNDNDDDDDDDDDDDENSDESDEDNNTKQNKKKVQFQKQQKGQKQKTEQTKKSKAKKVESSDDESISDESDEENQFNQIQKINKKDPSIYSDGSNEEERINRREKNFRFYFDQKNQLNQNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SKT1</accession>
    <name>A0A8S1SKT1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Calcium-dependent protein kinase 1</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="ORF">PPENT_87.1.T0080420</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cell membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Parasitophorous vacuole membrane</location>
            <topology evidence="24">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="30">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8140036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SKT1"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020002">
        <property type="term" value="C:host cell plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020005">
        <property type="term" value="C:symbiont-containing vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000398">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000299">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000383">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000828">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 26 27" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17" id="KW-0969">Cilium</keyword>
    <keyword evidence="15" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1032">Host cell membrane</keyword>
    <keyword evidence="16" id="KW-1043">Host membrane</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0519">Myristate</keyword>
    <keyword evidence="12 26 27" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0564">Palmitate</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 27" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="28">
        <location>
            <begin position="27"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="330"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="366"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="401"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="437"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00060437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00068067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="EMBL" id="CAD8140036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="473" mass="53991" checksum="5E37F3C5817BFAA8" modified="2022-10-12" version="1">MNQEEVFFKKQWFILSHQGKIEQFYDINLKKVIGSGTYGSVVKATLKGTKNQRAVKVIPKSKVKNPDKFKKEIDILIQLDHPNIIKLYETFEDQRNVYLVMELCEGGELFDRIMDKGCFSEYEAYEIFLQIMQALNYCHSNGICHRDLKPENFLFLTKADDSPIKVIDFGLSTLFEDPITSKQTGQKLTMKTKAGTPYYISPEVLKGSYDELCDIWSAGVILYILLSGVPPFYGDSDPEILDAVQKGDYTTEIPELKAVSEAAKDLIKHMIISPDKRYKASQVLKHKWMIESNKPTKQLKLNFAQLKNFNGSNKLKKVALTFIASQLNEQEITDLGKLFKQLDKNGDGVLTIDELREGLIGVSDSQQKDLATIIRSIDTDGSGTINYTEFLAATMEKSLYMKEEKLYQAFKMLDLDGSGKIDKQELQTVLGKSEKVIDEKYWDDMIREADKNGDGEIDYNEFIEMMDKFSLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SM05</accession>
    <name>A0A8S1SM05_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0100152</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SM05"/>
    <dbReference type="OrthoDB" id="306368at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8141503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="214" mass="24906" checksum="5058881639FD6BFA" modified="2022-10-12" version="1">MGNTCLHRFDNQKQKMIKINGYPKLNCPNKLILEPSIQNITQNLDTFEEDFFKSEFQFDIQSDNQRNYSTSKVKLGSSIEQINKSKQIECPSNFNEKCDFQQDNQLNLFMNPKNNNKNVFIQGQKTNHQKKIKQKVSLQKIEKPKSILKTHKKQHNSSFNSFQCAKCQHTVTFSLATCNSRNSRSLSPLLNSQSIDLQPVNSRSMTYLQQFPYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SPK1</accession>
    <name>A0A8S1SPK1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0090052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SPK1"/>
    <dbReference type="OrthoDB" id="1668230at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001175">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000683">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="96"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8140394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="506" mass="59613" checksum="DC417730B52B760C" modified="2022-10-12" version="1">MQNINYQENVKYYGYLLPNQLILATVKQSQTPKYNVPLLLTTLIHFISQQHSQETEQFGFLYKEQFKYFYANPNIILQLKELLACKVMFKEISDFYYPISMLGEEVYLVMKKGSKDQYASKCVDKRYLIEDGGFNRLFNEIQLMQKMNHPKIIHLEELFEGENKFYLILKYLQGQSLHESFNKRQVIFEQEQIQTIMFQLLTALQYMHSLGIMHRDLNPENIMFKYQNTYDELKVVDFGLATSIYAETYPYPKCGTSGYVAPEIANLKDLNQKYDLICDMFSVGCIFYKLLTGKELFPGTDYKEILKLNKKCQINDELLTLYRAPKEAIELIAQLLKINPKERISDQVALQHNYFQSKFQTKRGKFQQNNNKYTPAFQTQNFHPKDNHQQQNFHQEDDIVEEENAYSLKVPVMQNMKTFGQFVCKIKQQVTPTLARRNFKKFYTSDFNSALSPNSPNPLLTENKPSLIINSQNQELVNEINTKNEHQINQMGQINEEQEDQLQQNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SRI0</accession>
    <name>A0A8S1SRI0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0110223</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SRI0"/>
    <dbReference type="OrthoDB" id="308818at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="142"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8142560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="496" mass="58774" checksum="149662F3B05A5752" modified="2022-10-12" version="1">MNNLKSKNSAPLDKPQKYPNSDILSIPMHQMRIDINNNNKNRLDSQQSSKNLEILRQSHQSSTSNLRLDNPLNKSSENLIKKTPTRTKVCTSIDKIPYPLQNNFSLSPHSAFIKPQLKQEDDQQNIISFCHQKSNLIIDHRKEQIILQLQQAQQLLVLKEQENQKLQEINKRLYEENQKLYNQGDDMLKDIETMRQNFDQQNNEYMMLKQQNQQLIEDVKLIQNERNQTQSHSDQILNLKNYEIQQLQNELREYQQLLQNKDQTISNLKQKLDIVEQQEICRQNLKQSQKSSEKSYLNNNNQQIETTQKIQNQDLNSKGSQMQSDIQYYQIQLEQISSEKDICEQKLVELQTIINQFVLNEKQQNITSNSNGNVIYQKQYNKLKQIVQIQKKQIESQQKTIMNQHYEIQNLQNLLYSIEQDCSSQKNSNIFYEKFSIQQPNICDIGQKESNEREPIDKIGLQRILFKQQQDSNVVVEDTFRNMQKEQELFTSTYDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SUQ6</accession>
    <name>A0A8S1SUQ6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0120023</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SUQ6"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000654">
        <property type="entry name" value="Protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="17"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="293"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="406"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="293"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="322"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="341"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="364"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="417"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8143069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="635" mass="73726" checksum="F4A345CA13BFDB3C" modified="2022-10-12" version="1">MSFLQYPPDKKIQNYQFSFKAKVGKGAYGTVYAGKNMNDNKIVALKIIDKKILQTDYANQLIASEIEIMKLIEDKNIVKLIDVLQSVNNTYIITEYCNGGDLREYLKKRKLISEVDAMNIFRDLLHGIKALLKIGIIHRDIKPANIMIHDGEFKITDFGFAKQVDSHIDAIMNSLVGTPLYMSPQILKRQSYTSKCDIWSLGLILYELLYGITPWHSSNLVELMAKLDSKPLDFPCFPRVSDQTKQIIRSCLQIYEDKRISWDGLFELTNTQDLINIPNQSMLPLIQKHPIQYQSQQNQQNNQATENIEKGPHQRTKLRTESMGNCNFTSRHNRSLSNANNHDKDKSDERQKVKCTTPKLSYLQKVTPQRQTTTITSDSERERSNSIIKFANKKLSLMKYLENAHTTPQDQKDNNEQSKSPSYLQSKSTNTSNRHIQDQENSSNKTKVTEFQIEEKTRDPINNIMNNGYYNFNQNSKLKRTFSNNFKENSLSPMGQNQHGIQQLLKLIQNQYDLIPSHFIIKKQIEQIIGQHRTKIMQTKDFQYTFEDTKELKKIINRLIDYLNEHPNDNFAIQLSVMLLLIINYNQVVNYCLQQKSSDLSKTLLDLIKKNQSIQQKQLILNPAFLRENINKQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SVM4</accession>
    <name>A0A8S1SVM4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA/rRNA methyltransferase SpoU type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0120228</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="241340at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016423">
        <property type="term" value="F:tRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18091">
        <property type="entry name" value="SpoU-like_TRM3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045330">
        <property type="entry name" value="TRM3/TARBP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044748">
        <property type="entry name" value="Trm3/TARBP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12029:SF11">
        <property type="entry name" value="METHYLTRANSFERASE TARBP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12029">
        <property type="entry name" value="RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="tRNA/rRNA methyltransferase SpoU type" evidence="3">
        <location>
            <begin position="1247"/>
            <end position="1388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8143436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1396" mass="165465" checksum="C35FEE8B63FF9215" modified="2022-10-12" version="1">MFVKELLQIQEYVEQKKSSGKHVSLKPLIDICVHKYQKDEVQEALNQALINELKSEWTSQQNIDILILCYKRLKCELQMELIERTFEISIDNLQTTKRILSICTDEQLRNLCSKYISTQGYIDKIIKPIYESLLNVNDQTMIIVDKVKKLYKNLFSLLKMQKQWVLDRAMEFVLFSEDIYAEKSQKFVAQQFQEIRSIIYAFIINDDEIRLNFIKYLPQIFQQYNPKNIDFKLHCSELIKIALFSKSVKQLMVYEQFWQALHDSYQEYSDLTNNKICVAVLNEVLINIDDEKIQQHFKLFTDLFITLDSFGTHLTKDVWIKMDPFTQQQQFKIDNELKRQLFQNIKPLFILINKASQHTNEKTIKNCIKSLFKKQIDVNYLKPLIIEIIIPVLNKPVFYLDTPLEGDSKFFELLKQFLSKIPYIDDVIIQMVQNIQYPLAVGAFSVALKDIKVEITQFSLLIQFIEQNLMRLNLRKRIQLLQAILHLFKQNTNPEYLFQLIKVIPFDCLFLNNIGELLKSLITEDFLQYAQEQQQTFDLNSNYHQFAVIYHLTQDYKLFLTFYQPIISQLQQIYTSTYTQKEIKHKILEQLNYILKTSNQVQFLEQISSGLFAYLTLDLEGAMIVTEELWITSLQHFELVPLLTQQMSKFVGFLFQQQFDNFNIIHLELLIKTLKATNIALQQAKRNKSGLFVEELFLKYGLQLLNKLQSIEQDRQLKNLNLHYLSHILTLFREVNHKPQEYHIQVQNLINNIIDITDQFNAYDSITLYQLLRSVYGPEIAFNKEQSEQFKQVAINSFQQMMHVFGTHTNLDEILAYIDFIIDPCYFEGELYEAVEQIIRVIMKQHQKQWVIQRKIVNNLIICLNKHPNRLPTFYYILFKLIKGSELRGYDSSEILQSSEQYVPLANNVKHLISCKGEFYGAYPRFLVFNFLFELGKQGGYEKELLYYIKFLIEVSIKDRYKNAYMQYTKQFRMKLHVWQNICCLKYLLKQENYQKVFGDDKKYLIELEQLIWKAFDTLNNPQYRQYIELMMSFLISNYPDIWAPRILQSLSDPKLKVNIQIPTTFISCIFLLHNQQTKFSQKFFDKLFPLVSSNVAYIRSLCQTTCLQYIKKQKLEYKDFIEYFESNRECQKIIKNISALIERYEKLMQTFDLDGILTIQFDTFGEIIHPSIIEILKNTCTQTIAELKSEDNMSENPPPPWFQYVQRQQLSTQYSQQLNFQRKINLIMDQFDLFKAHQPRQIVGDIIVVATLLEKIPNFGHLTRTCEIFGCRELVLPNKKILQDEQYQAVSVSAEQHLPILEIKEDHLMNYLQLKRLQGYQLVALEQTSQSKSIVNFKFDKKTVLVLGREKTGLPIEYIELMDNCVEIPQYGVIRSLNVHISAVICVWQYVQQWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SXJ3</accession>
    <name>A0A8S1SXJ3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0120489</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SXJ3"/>
    <dbReference type="OrthoDB" id="15735at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GAF" evidence="1">
        <location>
            <begin position="44"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8143957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="155" mass="17763" checksum="4CBAEF7E6CB09C28" modified="2022-10-12" version="1">MNQPNKVQEYEKVLDLLEHFLKKYQNLGRNAIETSIVSLLKNNISGLFFVGFYEVIDENYLEVGPYQSTILATPRIEKGKGQCGQCWAEGKVQIQEDVKVCQNYIACDNETQSEIVIPVIKNGVVQSVLDIDSEHLSRFDEVDSKYLQRIVEYLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-02-22" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T2I9</accession>
    <name>A0A8S1T2I9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0150068</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8145888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8145888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="147" mass="15877" checksum="7CC6E7FAB5CD3B7F" modified="2022-10-12" version="1">MIKFSWXITQEGFKGGKWKPMDAEITVVANGVTKSVKPKKLDKGQNGEQMYQLKLNFLGNKVTIGCEAQMGETTAARILQEAADDPSVVGSTSVSCIKETSSSTCPTEEQVLTYNSENCSGVSCDDDESSNAMIFKVVITMLGLLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T4W7</accession>
    <name>A0A8S1T4W7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0150463</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T4W7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035772273" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8146646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="198" mass="23291" checksum="91126BA701C58561" modified="2022-10-12" version="1">MDQKKNCTFFFILILSSIVRKLQINASMDIYKGKQTVKTQAFAFGKMMFVFQTWCPLKRRGGNKRKMLGFCRRRLQSSNVMQILLGLLLNLCKLQDLQQRQLLIWLLHLDLKNGKKITSNLFKELPCAKKILMAILQMIKVMDVQNQKNYVRNTNMIFNVLKVIKSQIVVFEIIKMKKVLKKFVKIYLLLKIMNANLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T791</accession>
    <name>A0A8S1T791_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF4378 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0170069</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T791"/>
    <dbReference type="OrthoDB" id="307888at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="64"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="411"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="489"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="176"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="686"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="65"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="90"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="104"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="272"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="411"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="494"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8147648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1116" mass="132187" checksum="B94611F52C9B4490" modified="2022-10-12" version="1">MNTYSSLKSFDSQIQGRKIQEPNVAITSNRAKKSPRLCSPEQSLDQFIELKPQHMQQTLKKKKVIQGQQGKSKSQINTAKKGFFPSQEKKLKKSIQHQTKKKSNTLFLSNSTSPQKQMNPKKPVQINNIGEMLIKQQINKQNLQKDVKQLPEKKNQNTVEKRQNVQKDDIQLYINCKRAQDTINLKNQQIQMLLEQKKKIENLFKLEQLRKKILSKKKTIDTRIKSPKKGKKKLKKNKQKEQIEKILCSNQEKPGKLIDKLIKRQEQHTKMEQNQQQLKQEQLQNKQNKIQKQIDLPQYDEEHSFSYIEPTDQILPNEVEDTLNQFPPSNYLKELVKNLIQNNEVLATANLAQHDFKLLEFYYMQAAATKIQSVWRGFYQRKLILDSYLKYLEQEDQLQQENQQYQNNSFLQESNKSSSPKVVNPPQSQNNSVFQDEEEEDDSHNKQLDVQQAINKILEKQQSGSPLNQSSQQFSQQQSDDLVVINQVPRGSENEDNDQEIEENEDMESSKNIHDHKISKQETIIQEIQHQLNDWNFNLENLITENRKSYAIKTLKEQMQQAIISIVQSQIQKFQEQQSKQQIETEQSVDLKMRLSQETNTEKNFNDIRKKFKSVQEQSEERLLQSSKDQSTDINRSSLLALQSQLMRREIELLSMREEAIQLRYQAEIKKYENDDNKKLELNFWLKKELEDLQQTKQAIEISTKKEASAMKKIQRDLIIAQSFDENNSKLQSLRKRVDEQLSNLKQSKQSISDIKLINNFCVQNENELEKLVETQSEDFNYEDQFQQKQFLKDNFIESYETIEKSAKVNLIVSDLVIDLLQEMTEELFTNDNKFEIIIQNLIRQSIIPQIPTSIIDIRYYIHNLFDYILPQHYYEIIRNINIPYGFSPQKRLQFIHGYMESDESQYNEFLTFILKEEYFLEYEQYRLQQNESENEDSVTQALKELEHIHNRAIFDACNEALNFQRPYYCNSGSPYPWEGKIGHKVINDNDLSEIFKNMELKVVQWANSLCGFLPVEEEIAISKEKQVILDEKNQQIMLQQMQYQDNPELMEQDYQQDPISQIREQRLYKALIQDFKDLEYRWNQDQDDKAELMMEIGDLIFEQCIEEFLIESVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T7J7</accession>
    <name>A0A8S1T7J7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0160342</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T7J7"/>
    <dbReference type="OrthoDB" id="74764at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="129"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="483"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="484"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8147366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="526" mass="60526" checksum="73CC69030250739A" modified="2022-10-12" version="1">MYQKLWMPIPNGFKFNNERLYIQGEFTKENGKIRVLYGYDQYILSSKKATLPNKCLKLDFDTKFEILRQAIPKKDEDDDSLGPIIGLQFLRDCVENMVKQRLNAGVQQIIEWRSFLGERINQWQFHNMFRVYKKIGKGNFASVYQAERIEDNQLMAIKAFAKQPAYGEENGKNAIINELTIMRNLNNTHLMKLYEVYETNNSLYVGLELLEGGSLYDLIKDKVLINTKQIQQIIVGILLGLQEMHKKDIMHRDLKLENILFKKPKKMESVVIADFGLATYVNEPVYLYCRCGTPGFVAPEVINIKDMKSKYSSVCDIYSLGLVFYLLLTGKSAFNGKSYSTVVKQNREANVNFDIKQLQNAPGYAIDLLKQMLEKDPNKRINAERCLNHPFLYEIVKEMIQVEHQDQNFDEIDESIEIQSMMHKINDEYSKFDATRNVNSPLQSPKQSPGMVMQKQIKQQKQIDSQKAFGADSPILKGKIESVDSAQTIGTPTKRSSILQPSPQIKPSKFSKQGQNTPLLKYAKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T9K7</accession>
    <name>A0A8S1T9K7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0180420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8149110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T9K7"/>
    <dbReference type="OrthoDB" id="20727at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="154"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8149110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="359" mass="41098" checksum="9E499E9B427E383C" modified="2022-10-12" version="1">MKIVIRYYNDWGILNVEQLKISSKYKISELYDQVSNLTGIDQNQLKLILIQTNEKVTFQIKKIVLDELNLYICDYQIQQGSAIFAQKTTDPFSDMASSFSSQFDPSQFLDTSANSIPEQETIFQLIRLSLLSDLELFLEDRNDRAQLINGIELTGWNALHLVTFLGDEKMYLWLISSGGDISILSKDGWNCLQIAIWQSNIKMTNLIMGSNSINVNQITIKGTALHIAAQIDDQELIELLLKHPKIDISITFQGKSVVDVAGDRTKKILQKELLILRNKQFDIINSFRQSICTFYSLNSFFTNRPQKPPIIKGSAKIVSYFKLLLIDNYLICDPDSGAIARYKTSIQYPFNPKYIIYIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TAP7</accession>
    <name>A0A8S1TAP7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TIP41-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0180180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TIP41 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TAP7"/>
    <dbReference type="OrthoDB" id="10253878at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051330">
        <property type="entry name" value="Phosphatase_reg/MetRdx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007303">
        <property type="entry name" value="TIP41-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021">
        <property type="entry name" value="GAF/PUTATIVE CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021:SF16">
        <property type="entry name" value="TIP41-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04176">
        <property type="entry name" value="TIP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8148667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="336" mass="39573" checksum="3E2ACCAA21767875" modified="2022-10-12" version="1">MNIISHKTYNDVNIEEYEQNGWNFIFTKAGMYNTSDLDMLQDALTLQGIPDITNGNNCGLIYNKQFNFGFLVDPKDALYLCNYQSRETNYLDIKKDVKSNKLNYISVIPEQAQLKYANIWNQKQNADIKVLNKISDTFYSSPYKGTIVDGDQINYQFQRFKQITGIQGKSIFEKQLNENVIKNEIDYLNQVFNIKSNTFHCEYTEDEIPLNNLTQANPIKWASHISLFEDELGDNGLSNCEYRFRVMGDCFFGLIRSYLRIDEVLIRIFDTRIYHQFEWDYVLRDFTHREDSWTNIQKNGFNFTPQWSTDQNQSFMVQNSVPIQLHVNDKIFFKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TC83</accession>
    <name>A0A8S1TC83_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0190129</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TC83"/>
    <dbReference type="OrthoDB" id="70770at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 5-kinase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF263">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8149478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="424" mass="49218" checksum="08D1705C3E18A397" modified="2022-10-12" version="1">MQKQEPLKSGRIQNITIKQSPQNHRMAIKIQSAYRSYITRQKLKQNQIFIQSLKLAEKGPKNQVKCNSTEDYIIEYHTNSYSPKINQSDVSKTLSSIQNNQQQRAHQNFKRQLSLKLKPKETDQSDFEELNQNDQTVFQKYLQSSKNCPGTLKNQKEKINSFNFRCVLTLEILQANDQIKLKQQNHKRQKFDTIKLIGGNTYNGEWLDSLPDGKGKYIFSDQSYYLGEFVKGLFHGKGEFKSKEGTYYRGQWQYNKMHGQGTYNYNNGCKYEGSWERDLPNGKGIEWYANGSVYVGSFLDGEKHGTGKLTFNIGEIYEGEFQFDNINGQGTYRWQDGRIYEGEWFNGKMNGKGLMTWSDGRSYKGEYLNDQKHGFGIFSYFDGRKYIGQWKNGLQHGQGEFHKGGGIQYITKGIWKEGQLVKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TIA6</accession>
    <name>A0A8S1TIA6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0200369</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TIA6"/>
    <dbReference type="OrthoDB" id="309683at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="72"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="228"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="14"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8150609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="389" mass="47134" checksum="0E5AFA83CF54AE32" modified="2022-10-12" version="1">MQNVKQMREKYENIKQQNPNRNVSARSYHSQTPSKEQYQVAFSSGQKSIQKPIIQIGKTLLCSPCYNKRLLNKQQLNKKQETELEKRLLMKQMEQNQLLEQQKINFEVGQLSYRKQLEQQNLVISKQLQDQQKEKERQIKEQEIQQQNLIIKQNQEILQKEKVEKKSKQNSLHQELASQLEQQKRLKKLDQLSQSMELDDPYWRYQAQEEKEIQRRKNYQMIHNNNNQQNHEKELLKKQQKEREIKNREIDERIKTLQDYHNQLEQEKIEKQQIQHQLILDLNAQVKLKKAKEEAIKLIEQKKQYQRDQEIIQQEKQRQYQEMCNKKKVNQEVIEGLNKQVEMQKQQNNKQQQDTVKSDFYILKPIVEKQTISCADCKKQQVPQQLSYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TP36</accession>
    <name>A0A8S1TP36_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0270289</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TP36"/>
    <dbReference type="OrthoDB" id="5337378at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001794">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="11"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8155781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="598" mass="71934" checksum="230B11AD3F313195" modified="2022-10-12" version="1">MNEYCFYDYVIQLDKKIGQGQFGIVVECYHKFKYKNIELCAKIVQNNANIDESMMKEIQILKKIKSINNPHLIIVYDVFEAENKFFIIMERCSGGELKHLIEQKKSQKQLFKTSEIIDFIYQFIDGYQVLYNNNIMHRDIKPQNIFVDKVQQYKIGDLGAGRILEDVKIKGDYTKIGSPIYSSPQILSLREFSSQTDVYSFGMVIYQLTFLEFPFRPVMTELQKFILSIQKQRFSLNNQPLKNEGIQREKDEIQFLIENMLVYDESQRITWEKLFERIRKDVVYEQFRSKYEDAQLLNTIIDWDQKNKKKQVKEAKMLQEATKLFQQVGSNIKFSNFIPQTSQLERKRSPLSQYLRIQYFKVVVINQALNQLQEVINEKKLHLQLIEYFLLISSIYGYYCNLLKNIEAIHKGDYNQLSEHIRDQKDKEQQNLQAINEYQQLKTEDDLRIIEQCSQKLQELSALSTQVVSQTIKKINEQKIRFQTLCDLREVLFKFSTYINYYKYWEFIRKFFNSYLQRKFNDNLNSDLQQLSDQLLLFMVYMYELCNLEEKHKQIELFKENKILIIPKQSYTRQDAIFYLEQKRNELQYQQINQNQFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TU14</accession>
    <name>A0A8S1TU14_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0260286</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8155047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TU14"/>
    <dbReference type="OrthoDB" id="203073at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8155047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="302" mass="34629" checksum="632A894A67BDF925" modified="2022-10-12" version="1">MGQECQKCQKRQEVHSNEINKQEVISSDSDPEYEEDDEKQPQKPPSMDSKSNSSTFIKEQYLSSDSKQEQIESSQNQLNFQDGGIYTGIVINNMANGLGRLVMPNGDIYEGSFINNKLEGDGKCIYARGPIYTGQFKQGKSNGFGKEVWPDGSVYEGEFRNGKKNGKGIYKWSQQSTYNGEWADNMINGVGKYEWPDGRSYYGQWARNQMHGRGYYRWIDGKYYDGEYENDKKSGFGIFNWPDGKQYQGYWLDGKQHGKGLMINKEGKKKFGEWQNGKLISCSDESNLQLIPDGWFLNTFNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TU52</accession>
    <name>A0A8S1TU52_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0260306</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8155087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TU52"/>
    <dbReference type="OrthoDB" id="313045at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="487"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8155087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="556" mass="65586" checksum="CC4DBE8FC259152F" modified="2022-10-12" version="1">MTNIIEESMIINRLTSPQKLNNPNQSPLRLTPIQPNSYKHSSRQSSKVNLLDIHNHKNVDQQDSDNVANPMSSQVDLTQDEAKENDIELDVKQVLTEINDQQTNSYQNLDEIQPESQVQQPQLIIMQTANTIQHQIQKHKQIIIKNTQIGNTIQNQHQQKPQQIVEKEDIIQDINKIKSLDKQMIENLKEIYTFYSKQAITTNKYNTFEKLQQLSNIMILQKFMFFCRDFQLIDLEINNDLILKLGGKIDELTNKKKNQFKFNHKQNFILTKFNLVEIYKKNANSQMELNKENFQIVIIKIAQLIFPEQNELLYEYLGLNNPKQYRKKMQIIGKPFNSKEQSEVLTQEKIYERKIYLPPKPNLRVESVKKERPIIEQQFPKVNLTQRCRRNQKQNETGIKDASGINWSDLDDLQQHFDPKKFILEQDLSDKEDDYYLQEYSKHGYSLKKKIQDQMMNQKELQIKIPQISIYSNILSSNHKCDNKASNIMLTSGNTRPSNNKSFDLQDNQQRKQSVGQSPQNVEQNSLEKQNKIYRAFLNQQSYRERMVNKKKLILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TUN0</accession>
    <name>A0A8S1TUN0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0260108</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8154719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TUN0"/>
    <dbReference type="OrthoDB" id="288503at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8154719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="60" mass="7012" checksum="DDC69A34FF9DD486" modified="2022-10-12" version="1">MGCSIQKPKQNQRNHLDQIERLIQSDCLSNESKFMINNNPIIKRRNCSKEKKNLQGSLQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TUZ2</accession>
    <name>A0A8S1TUZ2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0260168</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8154839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TUZ2"/>
    <dbReference type="OrthoDB" id="285771at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8154839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="158" mass="19017" checksum="8D5052F36295AE38" modified="2022-10-12" version="1">MCLEEVQDDKGLEKFLKMATKYYETKYGTPQKNLREKAMTLWLDLPSFKDKMIVMEFMQRKINRIESSQTKITQFFKSHSKPSATKIQKQIDIGDDQEITGPLIGDNTPLKRDRSPYSKFYEETYWKLKLEYEFLNQKEIRQMVKKQWESNRKIIQNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U8L7</accession>
    <name>A0A8S1U8L7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Myb-like DNA-binding domain containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0360205</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U8L7"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF69">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_38, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="55"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="59"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="103"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="103"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="2">
        <location>
            <begin position="106"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="154"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="158"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8161475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="26565" checksum="438F074955911D8D" modified="2022-10-12" version="1">MYINPMNPMFFCFPYFYQVPLPQNFVKEEASDTEKEQTVQSIQEQTNASGIVRGKGQLWSNDEVKNLVKYYKQYNGDWKKIIKHLKGRNISQCSQKYRKLQDQEKRTKRKWSNEEDRILLDSFELYGKQWIKIAEKLPGRTSKQVRDRYVNQINPIINHDEWNEAEDKIIMKEFQQNGPRWAQIAKQLNNRSENQVKNRFYYTILKKYNGELHPYLKIQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U9S2</accession>
    <name>A0A8S1U9S2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0350084</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U9S2"/>
    <dbReference type="OrthoDB" id="307814at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8160633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="272" mass="32188" checksum="1512206A8ED3747F" modified="2022-10-12" version="1">MNCDTGKSASIRLSTQLLYFMDYKIHEYSPEELYENKARLFESLMLEWNNMSANQQEKFCKKVQFTNGGEKAKQRKDESTLESLYETAVEKYEQGQQLIIEEKNERKASSKEIQLAEIIKEKKNNKQSKYSEDQKQEPLISNQEFSEKETNSQQPQFKSQKSLINQQIEEHHVDININQINEKDENIKQENEIDEQKNNLRMKNPEVGKKLSQKGTVERIKSKRSSIQKRALSPSKQIQLEQQKNQKYFFIDETLNLKQLKDQIKNLLKQNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UC54</accession>
    <name>A0A8S1UC54_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0360244</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UC54"/>
    <dbReference type="OrthoDB" id="285573at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8161553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="333" mass="39228" checksum="75409A1D22335DB0" modified="2022-10-12" version="1">MINYHINTLRGIAIEYKPVLFEDLFFDKSHLSTNKIGAISLQNLEQLRHDPKSIMDNLVINDVTLLVHELQNMPQCLICTSCNDFDKLNRLLQYCHHIDIAIGYQHNISKQLNNLPLYSVDNLIKKLEMEITMGTNNLKPAFIGVINFEEIDFNNQIWINIMESYIQCSENCGIPIVVDHNHKLEFKEMQQKYNIRKAKFIILNPYIEVKIIKSNYCIRREINIDSIDLKQKIVFNKNEVDYELYNLLPVYFTIGIKYKTHLRMFGGNGFEILKSETQELFDLLKWSSQITKLVEIKMWKCPICQKEQGENEERFKKMGKEFCSMKCLRQGLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UEW7</accession>
    <name>A0A8S1UEW7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Galactokinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0360054</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UEW7"/>
    <dbReference type="OrthoDB" id="187738at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004335">
        <property type="term" value="F:galactokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000705">
        <property type="entry name" value="Galactokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019741">
        <property type="entry name" value="Galactokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019539">
        <property type="entry name" value="GalKase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006206">
        <property type="entry name" value="Mevalonate/galactokinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00131">
        <property type="entry name" value="gal_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10457:SF7">
        <property type="entry name" value="GALACTOKINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10457">
        <property type="entry name" value="MEVALONATE KINASE/GALACTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10509">
        <property type="entry name" value="GalKase_gal_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000530">
        <property type="entry name" value="Galactokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00106">
        <property type="entry name" value="GALACTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Galactokinase N-terminal" evidence="7">
        <location>
            <begin position="9"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="5">
        <location>
            <begin position="91"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="6">
        <location>
            <begin position="346"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8161206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="439" mass="49297" checksum="CD0E22E96467A147" modified="2022-10-12" version="1">MNIQQLIDLFTQRYNGIQPQFLVKAPGRVNLIGEHIDYMGYGVLPFALEQSCFMLFAIDGNDIQIQHADDQQFKQFNLSLNPKEEYNEIQNYVKYVLAGYRAGIQYGNANQGIKILISGNVPFSSGLSSSSSLVVASSLMSLQVSGKTQQDIDRHQFVNQIIKFEREAGTACGGMDQTISVFGQEGSALYIEFDPLRLTQVNLPKGYSFIIANSLTESIKLETLGKHYNKRVVECRIGIKLIELALNSGTNFKTLKQLQDHLQLSLDSMEELCKFIPKGEIDLEQLEQLKLPVLLADIPYFELVLNQNKSVNPYNRLVHVVKEAQRVIKFKNICDSKMSDDAKSILLGQLMNQSQKSCKELYECSSENIDKLTSLCIKYGALGSRLTGAGWGGCTVSLVKETEAKNFKNKIIEFYYNHIENQDHIFATQPSQGASIIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UEY5</accession>
    <name>A0A8S1UEY5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0390231</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UEY5"/>
    <dbReference type="OrthoDB" id="291181at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="46"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8163458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="521" mass="62231" checksum="B956820433D91411" modified="2022-10-12" version="1">MNIIKYYRKALLRPAYNIFWRKEQNQQKSGKNDEKNQNQTGEWKGKEEYLVDLRKKREEYEKMKIKNEKEVRTQTIRQAREHEVNYGKVDFAKVFIDNINIQYSSEFKHPEEMFNFLGTLKVQLTEQNLMTCVNAFIQFADRITEDDLIRYEFQEFQAILSKQIRIISNVENIVKITKLLDILCLPSEHEIWGQLEISILNRMSKLNLEQLLEILSHLSNQKEGSDQFWDQCENQIQDQINKIQDINQQYQCVIQTLMFYWRVQRGTKQFIRGLVGIMLQNSQDQQIYQKLPMNTIIQGVLVMGQLCEKQDTSDIVKSQQFKNYFIGIEQYILKQFDTLNFEQICLISQGFGFEFGSEILNQKLESKILENFDKYELQELKLIIKSFLLSYRGSKKLFKIMMNKISSLKHEFTSIELAEIIKSYYITENDNQEFYVDIERKILQRLKEVREITPQEIYEIAYSYLITRIGSREFYKLLEIVITFRFDDLRQNAILMSKLHDLYLKSALCDTKLIERMASVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UFV0</accession>
    <name>A0A8S1UFV0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0380155</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000038">
        <property type="protein sequence ID" value="CAD8162619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UFV0"/>
    <dbReference type="OrthoDB" id="10252354at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004708">
        <property type="term" value="F:MAP kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06623">
        <property type="entry name" value="PKc_MAPKK_plant_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000040">
        <property type="entry name" value="Dual specificity mitogen-activated protein kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013:SF9">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="55"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8162619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="331" mass="38741" checksum="E04B94292E9F2C36" modified="2022-10-12" version="1">MKLDINCCEDDDIMNQFSENGSLIFQGFRLNEKGIKYHKQCKKDQKQQNITLNDFQLVTKLGQGGFGSVEKVQLKGTQEMYALKKIKFLSDTNQEKLLTRELDALISCESDFIVQCYGAFYSQGYICIWLEYMDLGSLDRLLQKDGLIKEPMMMMITYKILQGLDYLHYKHKIIHRDIKPHNILINSEGNVKIADFGICQTVSTGQYLNTYIGTAIYMSPERLQSMNYGMDADIWSLGIMLIECLSGQHPFKKKDYSQIQQMKQIMEFDAEQYLQEYNWLPETKEIIQKCLHKDPKQRPTVKELLQSKPMQMAKNINEQVFHQWLKLKFKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UI65</accession>
    <name>A0A8S1UI65_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0390217</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UI65"/>
    <dbReference type="OrthoDB" id="314832at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8163432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="120" mass="14295" checksum="EEFAA5C951C1F921" modified="2022-10-12" version="1">MNINEDETNKLLQEIRNEVIDFTTANFLGQIVEKYQNQENICFKENKGNRFEFVKCMMNFQKSQQKEEKKMEFKINYLKNEIAECLSINEKSQCQQSAINSIIQIQQDFLKSLELTLKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UJS9</accession>
    <name>A0A8S1UJS9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0410180</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UJS9"/>
    <dbReference type="OrthoDB" id="300566at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00106">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF15">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="9">
        <location>
            <begin position="18"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="378"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="554"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="95"/>
            <end position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8164725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="605" mass="69619" checksum="981037EB457E8B68" modified="2022-10-12" version="1">MNKSKSPNQRDIPSDNSKVRIAVRVRPTLNSESEEDFVQLIDDNTIKVTRIGNSLRMKFSDILPKTANQNDVQRLVSESIKSFIQGINNTIFAYGQTGAGKTYTIMGGLPENVANVSTEKFHQVIRCNERGILPRAVQQIISQLQTQIEDDQCRLYLSFYEIYNEKIFDLFNLKSQGLDIRENKNGDVNIPDLSQIRIMDIDTAYEYLILGLRNRVVGCSHANSKSSRSHCCFQMTLQQVTVINGEPVLQESSLKIVDLAGSEKFKIPTELTPEEKELHIQELTSINGSLSCLGHCISALIDRNRTHIPFRNSKLTRVLSDSLSGSGKILFIVCVSPSISSSAETFSTLQFANRAKRAVLDGRNIQQPKTNKPKGVSLEEYQELLKQYQKEKQMREELEVIVQKRNQGELLQQISKLKQQNQELQEQLYQVQQQQQQQQQQQQPININYQQQQQQQQYSPLFSDNKKSLTINSNDKKVRFADDFLTAHINNMDREDSQVFENLAIFQELKQLENKDKKKIINFEEKFKSYFGQSEYQTNDLAQFNTGKQFDSIIRKVNDKQNNISNIVQDLKKEMSKAVNQLEQLQKEDRELKSADSCKENFIQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UJY8</accession>
    <name>A0A8S1UJY8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0420173</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UJY8"/>
    <dbReference type="OrthoDB" id="289272at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="201"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="11">
        <location>
            <begin position="457"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="565"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="568"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8165358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="608" mass="70866" checksum="D11F670AC278406F" modified="2022-10-12" version="1">MNNYIYHINKRRLLKSVTLINLINTLYVLNQLQSYNNIIMNLSKSISQKGFRILQKSSLSPSHKLSPEKSKLSFKTRKNSELLKLMVSPKLITNQTTQEGSFVKRETTLQNSQIMDLILVIVHVGQQQFRFTFNRNITTTQIRAAVLQKLKLKIVALNTIDHNILIDYYLTLEERPIIWPELQLECQEPVVTGQHVSLKDFQILKCIGAGGFSKVYLVKSKMNGHFYAMKLVDKEFIIKYKKAELLQNERDIMAFIYHPFTIQMLFSFESRNFIVFILEFCSGGELFYQLKTLKRMSEEQAQFYFVEICTCMLYLHQMGIMYRDIKPENILLDLDGHIRISDFGLSKLTSPDEFAYSFCGSPEYMAPEMLLKRGHTIQVDHYCLGALLYELVTGLPPFYSKNTQKIYDSILNEQVTFPQFLSQEIKDLMSGLLAKDPHKRLGVRGGVREILQHKWFKKVNLTDILMKRVTPPIRPDIQKLNFETKNLQQGDLEVREKLIGKAGFKKDFKIFDEFYFDYRENVRIADQQRLLKEHIKNVDSIQQQKSRSLDKIISEDSRQSKLQKRLEQQTSLRTSPQKGSTLMNNNFMQTGGLKTESSDLTSSKRRIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ULK5</accession>
    <name>A0A8S1ULK5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0410119</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ULK5"/>
    <dbReference type="OrthoDB" id="284481at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8164609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="139" mass="17046" checksum="AC970E7D0EB5BB83" modified="2022-10-12" version="1">MPNEFILQQTSHPEKKFNSKTLIGNWYEERCEPKSKYSTFYKEQKFEKNQYNVSKLTQESFMKCSQNWLNFQKNQQNHFQTTNQQEFKNPRDQFRTSEIKKFIIKKGVFEKNPQQMSDYRTKWTSAPHFFDRTYLGQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ULU5</accession>
    <name>A0A8S1ULU5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0430203</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8165940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ULU5"/>
    <dbReference type="OrthoDB" id="205403at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013865">
        <property type="entry name" value="FAM32A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13282">
        <property type="entry name" value="PROTEIN FAM32A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13282:SF6">
        <property type="entry name" value="PROTEIN FAM32A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08555">
        <property type="entry name" value="FAM32A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8165940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="323" mass="38095" checksum="D6EC15C770B40EA2" modified="2022-10-12" version="1">MALEIDMIHFSIQLLIQIIFRFGQQVCYQKMQYHPCTLPQYRNLDLRDSEFKGIQPTKNVHAPDIYLLLMSLVIFVLKSWQQQQGLIQSMFVINSFWKCQAISLLELQSSKQYFVFFLELDLRALIIFNYLINNLFLLIGTIYVLIFQVMFVQQPQKYITLNIIRTALEINLNHLSELSSFVQNIIIFLSMLQSCCAWILLFFGQSNILFQMSQKFGPLQLKKKIQKTNPTEKKPYIQQETVEKQEQENEKVKKQQFNPVEMAQQGGTGKTQAEIQYDLVRLKRLGEKIEKKMDLTYRQKIETFNKKIQKMPEHYDIPKVGPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ULW7</accession>
    <name>A0A8S1ULW7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">E2F/DP family winged-helix DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0430213</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the E2F/DP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8165960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ULW7"/>
    <dbReference type="OrthoDB" id="304566at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000360">
        <property type="entry name" value="Transcription factor Dp-1, a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003316">
        <property type="entry name" value="E2F_WHTH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015648">
        <property type="entry name" value="Transcrpt_fac_DP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12548">
        <property type="entry name" value="TRANSCRIPTION FACTOR DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12548:SF9">
        <property type="entry name" value="TRANSCRIPTION FACTOR DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02319">
        <property type="entry name" value="WHD_E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01372">
        <property type="entry name" value="E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="E2F/DP family winged-helix DNA-binding" evidence="3">
        <location>
            <begin position="76"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="164"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8165960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="271" mass="31778" checksum="7AF3DDF3CAD081B9" modified="2022-10-12" version="1">MDFTGQFRLDEDSMAINIFQTPIKGLVQGMFKFSISISYNILHANCNSQANTNKKLFVQTQPFDKPKIQSDLIKTQKKWSLKYISNRVMKELSHEAIAYSDICQQLTDEMIYDLDGQQNDRQKEIKNLRRRVYDALNVMISIGIVVKENKLMRKNAETQVNLTKQNLIIRKQKLKEQLQIKKASATTQIKQQESLKKLVELNKMRDVDENEKIRFPFILVKTQLNNIDEDELVLEQNKQMDYLKVFSKNSLDLQLDLSVVQKLFQSEHMIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UMD8</accession>
    <name>A0A8S1UMD8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0440003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UMD8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8166180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="299" mass="35576" checksum="F4AAF65B8BA612E2" modified="2022-10-12" version="1">MKNLGIRLKFRNNLGKMSKLLQSTVDSQLKNIICNFYEYLQNTQKILKFLQAQLDEFISLKSNDALRSLSINLQKLWSVEKYSNMMRQIYLYKLVVTPYMMEIVVQVLSEMIIKETAINNLKQNCINNFSKELKALYKSKYLIEMYQQQLNKLVSSNNGDENSQKVTLFDKVNLDKLNFLEIAVLFLEKIEQIQFQNKPSVLKKILNQRPNSCKYSNIWNQIIYQIKLQFKKKKKREAVIEAVIDALKELNLSNYDFYDEFINQHHQKQIEKQRKLGKSINIDRFLHDLKNIQLTLRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UNM6</accession>
    <name>A0A8S1UNM6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0400205</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8164066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044661">
        <property type="entry name" value="MED15a/b/c-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33137">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 15A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33137:SF4">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 15A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="328"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="447"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="534"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="725"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="844"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="931"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1267"/>
            <end position="1293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1580"/>
            <end position="1638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1651"/>
            <end position="1805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1873"/>
            <end position="1912"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2181"/>
            <end position="2201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2210"/>
            <end position="2229"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1070"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1928"/>
            <end position="1959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="331"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="545"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="728"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="942"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1274"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1580"/>
            <end position="1600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1615"/>
            <end position="1628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1671"/>
            <end position="1682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1705"/>
            <end position="1715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1728"/>
            <end position="1747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1766"/>
            <end position="1777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1873"/>
            <end position="1882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1898"/>
            <end position="1907"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2192"/>
            <end position="2201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8164066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2261" mass="262859" checksum="609207424CF20C7F" modified="2022-10-12" version="1">MVIVHSLNYGTVLIQNEKGGQIQTKEIFFSQPFQQIPQVAIALLNGHGDIFAEIHNINQEGFSLQMMASNEIEAEFAYLAIENDENYQIECFNRIDVGNEIQFPLIIQRPTFIGVLATNTHANNFNIDLLDGNLIVDNDGQLNMCIIITNEEQPIELIGYQNYQEQEVAEFHSFKKQNAMSLVQAQQSLEITHLDQHKSFEIWHHQSIEIIKDSSNEVNQTHKSIEIVSEDQPNQTQFKQQESIEIFKLDDQESQSNIDQKLIAEDINLDNIDDNLDSNNNQLEDIKLEDSSNVDQLTQQQTTEDASNQLEDITLDIESEQQNLQSETITLDDQQQSNEQSQQQLENNQQEQIEDVKLVTEELINNENSQEIKLTTDEVQIIEPKNESLQNSEEQQSVIDNQQVDQVQTEQIDQQNQDQDISNLSSEVVINNNDQVLQQEDVTFDSFDNDNVNQQEESQQEIQTEDIKLDDTTISSINAEDIPQQQDAIDIQNDQLSTQQEVQPIEDTTSTDDVVVHADEVKIENVKISEANLDDISTDQQVQEDNSEQIQEDNTEQIVQDNTEQTQQDNTEQIVLDNTEQLQQDNTEQIQQDNTEQLQXHHQSIEIIKDSSNEVNQTHKSIEIVSEDQPNQTQFKQQESIEIFKLDDQESQSNIDQKLIAEDINLDNIDDNLDSNNNQLEDIKLEDSSNVDQLTQQQTTEDASNQLEDITLDIESEQQNLQSETITLDDQQQSNEQSQQQLENNQQEQIEDVKLVTEELINNENSQEIKLTTDEVQIIEPKNESLQNSEEQQSVIDNQQVDQVQTEQIDQQNQDQDISNLSSEVVINNNDQVLQQEDVTFDSFDNDNVNQQEESQQEIQTEDIKLDDTTISSINAEDIPQQQDAIDIQNDQLSTQQEVQPIEDTTSTDDVVVHADEVKIENVKISEANLDDISTDQQVQEDNSEQIQEDNTEQIVQDNTEQTQQDNTEQIVLDNTEQLQQDNTEQIQQDNTEQLQQDNTEQIQNDKEDNTEQIQQNNTEQIQEEIQDNNINSEIKEQSEDISVEPDAFSEPQQKVTQINDDSLKQEQDIDEMIKFAQELKEQAKQLKNKSRNERQQAKLDIMEQANILEKEVGKLENESEEPQVVEDQISLDVNSAYDDQKDIQIEQPQEEQQPPIIYLNDSGVNPEQQLITQDIKDDDKLMISENSLQEQVDQELQSQEQQVETTSQIGEEDIQIIDASIKLDEDHQELEIYQSGQVEVIPEQVQPEVEIISEQVQPQVEEVQQTEQPQEQIEVEPQEDYVQEQETTKEEGAIEIGLTTDEGEAFIRKPKKSDFLREKQRLKDKLNNVIKDFKLDSVDSNTLDSVVEGDKQYSLIIDRLDVPENNEQVIVLKDEDYEQSQIEPEPEKEVKQLGDYLEAVEPNIENVNFIYDDEQQQEQQTIQIEDIKLNDDNTDEETNYKIVEAKDDDLVDTTLESIDDSQQYQLLSTEVAVTAEETSTANVENQSEDEINISFDEFQKELEVLANTIGNIKQENIDTKQNSDYIVVNDNSQIYEATYDEQSNDLPQEDEPQIQEDQQIYYQGFEDDQNNSIEIEQPENQDEQLQEQQEVEQEFDVPEQENNQEQLSEDQFLVNEQSTEDSAQQVETYDDAEPQDQYLTIDNLVKDSQAIAESYDQAPQYYDESDNESNEQQNQNVVESSLESDYDISEQQQQNQEIKEQQKSDNGQQQQQEQDFVVMKADQFKPQLQDEVQQQEQQSDIQQVDDIYSETPKDENEIDIIVDVGNQQDDQPQLSEQDYDYGDNFEIQQPAKNDAEQQSQQQQLQQQQQQQQQQQQQQQQQQDQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQLQQDSISSKKHKQKIQHSITISHNPQESNNKQTDRSIEIQHEDKSLKIEHPVNQEELKKDLKEQLKQELKEELKQEIKQEIQESHNRRKSPSLVIEDNVAKDPERPAQKAINNFNNLLNDVVDETLKEEDKKHYQKLPIVDVKKESFNKVEPKIEQGIEDLKKALGLKGGVEVVDNNGKKQVINVDDLKKESEKLLTRPERDNKNDSPQYEEFLKKVTEKPQTNNNDQFKNYEEQHNIRKQEILENIKRELEIKKGHFTVPNDSPQAELSPYEFERVYLDWEKNKQDIIPSMLQHSIEQPITQEMHSNSEVVQQKSSERVSRKEEVEKEVQELLYGNQKNRKQQSKQSSKEFKVEDLKYIKDDPLLDATYSGFLQVKANLRRRQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UQB4</accession>
    <name>A0A8S1UQB4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0470083</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000047">
        <property type="protein sequence ID" value="CAD8167368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UQB4"/>
    <dbReference type="OrthoDB" id="299896at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8167368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="149" mass="17847" checksum="D715C85BEBCCB91A" modified="2022-10-12" version="1">MQSEQEIFLKNGNKQMRQANMPQIKPRLWASIMLDKCDMIANLFQIFDQLIDPRLIFFDQDLTKQQSDIFFNAQALFPLFSRLEKSDPQICLYVTIKYGAKDIPGQFSGIIIFYNLFRFHIILIFKKQNNNLEYIKNRQNRKEKNMNRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1USG0</accession>
    <name>A0A8S1USG0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0460232</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8167107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1USG0"/>
    <dbReference type="OrthoDB" id="303030at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="8"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8167107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="236" mass="28379" checksum="3F8EB77AA6500FF0" modified="2022-10-12" version="1">MEDPSSIREISQQKVEQTKKKIQELQDYLAQQLVEYEKEQEKKKIQKIRNDLAKKHHKNRFSSFTNSLHRPQFINQIKERDLFDNNSVFINKDVPQLTNLQKSQLITPDRNQNPSYIIHQLDRPTNKQKSEFPPLRKRFLSQKEISRIEFWDKNFNHLQEIEQKEKSSLLIGKIKKQLIEQREVNVFKPTDQCLRLFDNKVSLISENPITPRQLSRILSLPKIKEKKVWMPNNNYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UVX8</accession>
    <name>A0A8S1UVX8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0490034</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UVX8"/>
    <dbReference type="OrthoDB" id="10249888at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000465">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="3">
        <location>
            <begin position="308"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="28"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="132"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="606"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="772"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="43"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="136"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8168333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="870" mass="100627" checksum="9CB30582C8070574" modified="2022-10-12" version="1">MLRKDSSYREISLTDSLEQKSFLNLARKPSNADFKGKTPLGVKGKDNKENSSARIHSDTPTQDFFFKREVIKSEKQLPNEKMAIYNDASRFQSSKTYMECRIEQLEQRKKTQSPQLLSMTPVQNKQISQERYNHLQQPQQQQQQSVQSSMNLTRKASVVPSRGLSALTSPTPKEGDFNIYKQQSSIPTSYLDSVVTQRNNKNYTVPEQQQQQQQQQQQLQQQYQSQQQYQSSQQQQYQSQQQLQQQQQQQQQDYKNPSQKLYYVTSLIKAFKMKGPNSAKEHLIHNMQGIIIAKKLKVPPVKKSIMLPATDKKTLLFDLDETLVHCNSNSFIPGDVLLNISHEGEKMEASLNIRPYTSHLLQTLSRNFELIVFTASQSHYANAVIDYLDPQKKWISHRFYREHCIPTDDGHFIKDLRIFTTRKLSDMLLIDNAPHSYLYQIQNGVPIIPYIDNKSDEELKSLLQYLMQFKNVKDVRELNMQQMKLYRFSEYDDPQELLKYEGEVIAKLTQLEQSEFGRYLIHTLQLEMETSDSLDHLINVLEKLEGRYQDDQKEDDANNRVYQDQCDSDIGQLDKDIANSQYSILQLEAKLEGNLIPARSIQQGVEKAKLAEIKQYTQEIQELDEERQEQNEVYEAKIAEHFEASKIIREAQAIIKDGLQRPSLAEIKKNGKNAIRIAPNILAQVTSSLSGSINKIRQTHRFTKAYASIFKVLLTIMNKSESTADEGSVEKIIQLCSDLLTKIDDSTNLERFAEDKRVQAYEKHKHLLNRDLQSAQSILANTQATLQSLNDSIQQAQNSIATIQQRLDYYQDTRQQRFVECEEAAYDYQQARSARDNNKQLVSDLIGLVNKHLRVLREQLALRVAAGDNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UXR8</accession>
    <name>A0A8S1UXR8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0500134</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8169047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UXR8"/>
    <dbReference type="OrthoDB" id="24670at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036157">
        <property type="term" value="C:outer dynein arm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036158">
        <property type="term" value="P:outer dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001467">
        <property type="entry name" value="Flagellar outer dynein arm intermediate chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002334">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF11">
        <property type="entry name" value="DYNEIN AXONEMAL INTERMEDIATE CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="479"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="39"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8169047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="650" mass="75804" checksum="888F5BB367AB4661" modified="2022-10-12" version="1">MPPKQQKVALRRPQPKKQPKIANQNLNQQEAEPDGDESEYQHERERERPQLTEKELNEDMPSKMLIPNNPQAPKNITQYDYLQRKYKTDELVEQLIIHFRMDGEIIHKDSNESRIQEELWETKQALIKEAERNQEMEDPGASKDKEAIKQTMRNKFNYNAREAQTDGRVIRERGVSTEPPPSDTLKGQITQWEIFDAYIQNKAKEESQKKKDHTSDNTVYKPSFKRCLKIMERTVVHNDQKEKYNDYKYYWSQGEVVETSKNEGHLLPIWKFSNEKQKKKHVTSLCWNPRYMDLFAVSFGSYEFSKQRMGLICLYSLKNTTHPEYAFTCEAGVMCLDFHPQSPALLAVGLYDGTVLVYDIRNKHKKPIYQSTVRTQKHTDPVWQVRWNPDISKNYNFYSISSDGRVMNWVLMKNKLEPEEVIRLRLVGKNEEESTLIGLACGLCFDFNKFEPHIFLVGTEEGKIHKCSRAYSGQYQETYIGHNLAVYKVKWNNFHPRTFISASADWTVKIWDSKISTQIMSFEQGMQVVDAMWAPYSSTVFACATQDKIFVYDLNVDKIGKLAEQKPSKQPRLTNIAFNQRDPIILVGDTHGGITLLKLSPNLTKSGVKASNFPDGKVPKEFENMPLEEYEKQKMENLLQVVSKWEREDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V0W3</accession>
    <name>A0A8S1V0W3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0520182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000052">
        <property type="protein sequence ID" value="CAD8170157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V0W3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8170157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="27212" checksum="ADFA66EDD974777A" modified="2022-10-12" version="1">MSYKNINYIASKFLKILSKQFNLQAQNLLLKYSLTCSFICYHFYNSQANFNFNSNYKIIGICRQITQTKIQYEKNLKQNRKILIYNLKRDNNLGFSTKIYLIELTLQALLFSVLLLLQISLFDILVIILPENTSIQLIIKHKYYKFYQATVKTGCLLLKIPYSDHLPYYLIGYSVILSYFLNIYFSFPNLDIVYSILSPIFLLREFQIDATLIIEIEDKSLIQQRQFRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V198</accession>
    <name>A0A8S1V198_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0530107</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V198"/>
    <dbReference type="OrthoDB" id="293187at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8170534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="346" mass="40461" checksum="6ACA17B9DD32AA9B" modified="2022-10-12" version="1">MSLIYKHHLILPEFKLNETIPFQNSQSNSINTLKSSQKTQITQRTLLEKILPVQHSLIENNDFSLPPIRKSNSNERPKLPLLTVRSSVSDEKTMKDYLDHLNTLRSQRNTQKQTKFKKVRTETEIVQYGDVKFKRVKENTSKKSIAVFLNIKNPQSQSYIKFSQQIYQLTHNLGKLKDYINNDRKLFQVIKQLIGLRKNLRKVMLKQNGDELIDFQDISKMCCVREIQYVFGEQKIVDKFDLSLYDDLVYLTDMIGNLLLQNEIQSINRISENIMKETEQIQKIQSQLGMGEDEQKLSVQTLPMKRFKIHDEIEQIEQKLGPMQLISKQVRQTSMVLSKVISGLND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V1E9</accession>
    <name>A0A8S1V1E9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0510162</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000051">
        <property type="protein sequence ID" value="CAD8169619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V1E9"/>
    <dbReference type="OrthoDB" id="284350at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="72"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8169619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="127" mass="14580" checksum="7E37A4AEE9EA9D05" modified="2022-10-12" version="1">MSDQSQYLNKSVKVICSDNRVIYGVLSCISSPFSIILQHSVATHPSPLDLDIEFHYTPQFPAKYFTNDESIREAYNNFNKATEETKQELQKKVDEAKAFNDQFMKDKYYVGSVVIPGEHIQNVILIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V2F3</accession>
    <name>A0A8S1V2F3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0520139</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000052">
        <property type="protein sequence ID" value="CAD8170092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V2F3"/>
    <dbReference type="OrthoDB" id="283111at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051175">
        <property type="entry name" value="CLK_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE DOA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646:SF11">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="39"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8170092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="355" mass="42074" checksum="843E7062A2867A18" modified="2022-10-12" version="1">MEWSRSREREKIKKQREKEETKKEKHYKLQKDEIFNQRYLFVQAISDGTFGRVVRVFDGGENDFKAIKIIKSVKRHIQSAKIEYGILRTIHQQNQYHPGNEKIVRAYEAFSHKDNYCIVFEDLGLSLYDFMRGKYFGGFELKAVQEILYDCLQGLDFMHTSGYTHTDLKPENILLIDKQQKGKKEGHYHVKIIDLGGAVFQTDCHSQLINTRQYRAPEVILRNKWTNLSDMWCMGCIAVELFTGQQLFKPKGNDFYHLAMIEKHCGPIPIEMIQQCRNEAREYFNEYQLNDSFLKWPDDQFMVQELNLMKVFKALIPQNQQELLNLIENLIVIEPLKRLTPKQALEHEFFKLTFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V2G0</accession>
    <name>A0A8S1V2G0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0500075</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM U1 A/B'' family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8168956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V2G0"/>
    <dbReference type="OrthoDB" id="296533at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030532">
        <property type="term" value="C:small nuclear ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12246">
        <property type="entry name" value="RRM1_U1A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12247">
        <property type="entry name" value="RRM2_U1A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000039">
        <property type="entry name" value="U1 small nuclear ribonucleoprotein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000029">
        <property type="entry name" value="U2 small nuclear ribonucleoprotein B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10501">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN A/U2 SMALL NUCLEAR RIBONUCLEOPROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="265"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="400"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8168956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="473" mass="55050" checksum="36ED764ED00C302A" modified="2022-10-12" version="1">MGIKCSKRKNRSFQNEIIQAQPIQISQTTSKEALAFCDSQDLANFMRTKPAKSYLQEVIQKQQIYFDSCDQLTHIIEGKSEILLRCYNDFTEKALKIKLMVLKYYKENFLVYQEQEPGCILNKDQIANETMQILIETNIILNCLMDKNFDVEQELWWGNDYFKDRRISLGEIEDDKIQNDQIVQPIVQYLQALKNKVTQSIPINQQYQKPFQQNQSVVNSTAIAQVHLTKFYNDLKLEFAKDAGEEQIPYLHPANQIIQDSAPKETLYITGLNDKIKLEDLKFVLYILFSQFGEVLQIVMKKTQKLRGQAFIVFQNITYATNAKAALSGMIVYDKPLIIEFAYKKSVIFDRIEGKFYYKQKQHKELQPNLPNELMKEKKQKKFDEKIQNSSHLNQGDINNVLLIESLPPFVTEIMLSELFRQYPGYSLIKLIPARGLAFVEYQNDDQATVALKGLSNFKITPECQLKVKYAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V735</accession>
    <name>A0A8S1V735_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0550121</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V735"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14014">
        <property type="entry name" value="STKc_PknB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="105"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="397"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="397"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8171629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="435" mass="50739" checksum="0BA2C10011C4DE40" modified="2022-10-12" version="1">MYRFDNALFNFPVTRKHFFSDKQYQLFYFKDQLVMTDNLEKQAKYQIKLNLTTQLNWIVSDKKIQAFEISYNNKMKAFYASPHNYQLLREMIAGKVFYCGIKNFYDIQMKVGQGMIGEVYRGVSLTGDYVAIKKCDKQKLASLQGGIPQFIQELQLLQQLSHINILKLKEVFFDQQFYYIITDFIDGKTLHTELLSRPTGLSISHTLKVMFQILNAVIYIHKKGIMHRDINPHNIMLSENVKLIDFGLARKIKNQIIFPIAGTPGYMAPEVINYKQDKPYDEKADIFSLGCVLYKMLTGENLFHKNKNNFQENKDGFFQLRKNPQHPECNSSKMDQLFVLLAHMLESQPNDRPSAQFSMMLLNEIDNNNQQIDRLIRKQQIMKPINMVTDDMSIEKTPRSFASNKKEKSNDTIPKSDDITTQGKKKLSRVIIKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V9I0</accession>
    <name>A0A8S1V9I0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0570095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="298796at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="244"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1043"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1237"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1352"/>
            <end position="1373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1552"/>
            <end position="1575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8172442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1616" mass="194048" checksum="BC8B2143B7B6F52E" modified="2022-10-12" version="1">MILVNHYHKQIKKNFNQLTRDSNNYNLSTLINQLDVSNIIFSNQQLTFIISIIQFALSLFFTILVIILLFYKNNNQTQQTQFTSLGNIFIRSIRILYWILCYPFLLVSTYNTFIDNNISQYFSIFTILFVVFFTFFHDFALLNDNFNKQDFLNIAQSRPYIFNSWIKLVQCSYSSYYYVNQNSHMTYLLVSFGLSFINYIQLQYFMVIFGKYDIDYIFHLVNAIYFGQSLGLLIQYYININDPYTLITIFIIITCVIIHTSFRKWYFEYQFTNSKDVPTRVMLLNWFRELECSDIYYMGIVTKHYNHPNNKKCFLRQNAIYYSGKKFAKYTYITTKKHNIEKYKGLFVKFLIKCLLETQLKLQPNSDEIRLLYSEILFYKFNLINQSFKHLSKIKNNINFNQKMRVKQLKLSIHNKLKENNQLSYRSKLPFENILKCEEQMKEFFQAFLKIAELQVYFWKNQLQQFISIPEQIKICQQILKEIQICQKNWKNIHFVNPIDEQQILIRFKWKFFYLFFKLYILHKKLKVQEIKELPDQNLKSQQIFSDEKVDERSSSEDEAQTKSYYQTNCIISSNNLFFRVDKFGEIIAASQSTLKILGRPQNEYRGARVETLMPDIIKENHNDFLKQFYKTGQSENLFKRRSILVKHSKGHCFKAQKYLKYIFNLDQDRFEYLSMLRQVTSRSQYIVLNSKWEIDSSSEYLYSIFGDYKLPFLSLCPKAISYTEYAQFLTNFDMKILSLKLCSFGSRERIQDKKFTFRRQNDQTFSNKSINELTSLVFKRELEEKQKQNVIQLFQQILEEDNEIERTAKLTRLNHIKLNIRIPIYKAEFQEDYNRYDAQMYTIYYDGESKKKLIFRNDNGNIRLDKFEFLQRYRWVKQQRMKFIYSKIKNLFEHFCQEKVLEKVLKVDANIKFYKTLNKISFYILKINRLELYDENTNNSDKQEIEFVNTSSFKPYTSALVREVVISSQSQSQYLKNQSGYQYSDMELISNREYTQKPFLIDYTLRDDFLVQGSNTIYSLNEQNDPFIKEEINKLSKKNQKLTLLIVLNRFSFFVQLLFISFTYFFCSFYYNPITIHNSVTLLGHLYKFQLLTVSSYNYIIDVALMNEHQLSNLLITSSTTKFNTTEQFYDFVNKEIDDYFTYICNIYSNQDFVSMVYSGFYSDDENVSGLDIFDQFQVNLLQFNQSDKSLIKQNNTILSHFRDYMAPYTQQLMQDSVISRINDAILYQQQSIDNLILFMIFQLSLMVINSIFSLLIKFKFRNDINTIYIIVTNLPKEDKIKAMKQYQILFAQLNSLFEEEQYGYKKQSLNILNADTIIQQDFEMNEIEKANQKRDKAQIQKPPKYQFNTVFALMFYLAIILILFILAYYFILINQIQTKQFFLDNNFLVQTNYFWLITLLKEKFIYENYLNSSYYSNITTFRFQEFLNDISLFSPHLYQTTIDDIQHVFDETLCDQVFDETGTYIYYNQFCDDILDGALKRGLKQFNILLSQVVIKALNPNDERYDNFTIEDLAQYQISFQIIYEIFLNVFQIWASNAETSLNNSSNLSLISYIIQLIVIIILYFCLLEYFLFTQLQQDFNFSKKYYRYFMLNETMNQVKTMRVDLVRTGLLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VC68</accession>
    <name>A0A8S1VC68_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0590222</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000059">
        <property type="protein sequence ID" value="CAD8173629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VC68"/>
    <dbReference type="OrthoDB" id="305288at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="441"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="736"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="443"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="454"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8173629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="902" mass="109293" checksum="BBF198E5C73B3243" modified="2022-10-12" version="1">MNQKKSYSHLEIHLETLETIWVQHSGFLERITQESGVNFYYQQNFIVLVGNLNYCEKAAQRIYTKIYDDFIMQIEQFRNNEEIKPFYQLFQQPQYRELSKGNQGFWIYSAQSLMKQYLNMESRGYQSIFPDLPKHILITWFPKNNMLYLQQQIFQMIGFRYKQQNLKAKYQFDVQQNVTALEAISIYLNLNLQEIMNQHQLLIELNQIPNQIFLVIKANNKNAIPETYKILEDHLERRKFWSIAYLQIKFTSNNLFYLKKNLNEQIRTNSIINLYTYEEVVLESKRLDQQFNINLNEIIVQRHSPDSLFVFGDDDKQGEITDTIIQIVRKLKQNNRLEQGDRFQQSYPYRESSNHPFLWNQNVNQKMSIPFQNNPKNYYQDQFAYYDKKQFEQRPIQQKFGTSINDQQSTHYQYPNNTLTEEIVQNEDQIDIPLRNYKRCYKNQDNNQPTHKNSNQKRYRQRQVKQEIQELDVSLYVPKFPQNNFVFQYNQQQEEEKSSQLLFIEELNKVIKCQVIKIELFQYQIIYTQNNCKNNMKINFTEFKSSFEEFMKKFYNQKPEIILEFDTENIKINVKFKQQEDIHQIKNIFHKYFLQQMYYVTQYSTQALNFIDILRLNSNVDYFSTKTELSGFLDEIQFQELVEIQEGQQMAIKIYNFSYRNYKLLKEKFNNFEISQSFQQNSSILDISKQVANNKKVIIISENQLKYLNQEFVDLEACQLRKYKEYFKMPFYFVQIEYIEELLEQAKVEFKRMNQQYVETQVQAKEKENKKVWMKEIRKDQSYQASKLYCYNMMQAFNIKLLNDINEIEINTEEYQTNQNLSMYYEAIIDLGDRELNPNDPKSIENLLFNSDCKFELKEIKLIDLPIGTFITLWIRNQRDILVEFSKIKKNDILPRGYIQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VD13</accession>
    <name>A0A8S1VD13_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0600100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000060">
        <property type="protein sequence ID" value="CAD8173869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="309910at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8173869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="165" mass="19916" checksum="F2C0DF69A872669F" modified="2022-10-12" version="1" fragment="single">MKGLNYKSTQTYSYQKSNEEMRRILECDGYQNLKLNEYQKIIEYTIDNSMQIKLSLLLYLQEIEFIRLSIAQNIQLCLDILMKYKQLKELNIQHNYLNSDRIILNITDRQSQITILPQKLNYTIHFVGYDCPFYERPDYINTFKNDDQSIKEIIINILQFNKRXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VGZ1</accession>
    <name>A0A8S1VGZ1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0650133</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000065">
        <property type="protein sequence ID" value="CAD8176540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VGZ1"/>
    <dbReference type="OrthoDB" id="292150at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8176540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="501" mass="59181" checksum="DD8DDE247C574F6C" modified="2022-10-12" version="1">MNYSQEFQVQKFFEPSQDVIKMIAKNEFLYIAYSNEIIVLSIKRLTQRKDIIKKKINIPKFQTFDTLTSAFNDNEQTKFIALAYREDSYPILYSSNLEKRIISLNSEQPQIKDFYVMEEQIIVLWNNGALVIDQQLMFDNVKAISCWNNNLAVYRNNELLIGNIKLNLNQEYKDEMIYAHSLLVLYNSSQILIYDDKNTFEIPFPGALQIEIIDEDTLYILCGTQIQSLKIRSQEKQIKLQLTFMSKFYPINEQDFLFKNGNIMISNKWSKINRVISVCKSYNQILEFACLKEQVGQSINCIIMEKDPQELFRLMIQPQLNSNFQQPLNINDFKIYYYYLKKEIMCLEIAKQLYEKKNKVQDQLLKQYKFEDLQKATGKAILNLLMLMEQHEVYKDFYNHIYISYLESIKPTNDKIKIQIAAVKNKFVPSLLRKEDICPICKQQLITWVTCPTHRFIRCQTTIEPVYLQQLFVCKGCDLQSIESIKVCPVCGCEMKRLLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VH06</accession>
    <name>A0A8S1VH06_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">diphthine methyl ester synthase</fullName>
            <ecNumber evidence="4">2.1.1.314</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0630170</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + 4 S-adenosyl-L-methionine = diphthine methyl ester-[translation elongation factor 2] + 4 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:42652"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="Rhea" id="RHEA-COMP:10173"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="ChEBI" id="CHEBI:79005"/>
            <dbReference type="EC" id="2.1.1.314"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the diphthine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.314" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VH06"/>
    <dbReference type="OrthoDB" id="2516at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141133">
        <property type="term" value="F:diphthine methyl ester synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11647">
        <property type="entry name" value="DHP5_DphB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.950.10:FF:000004">
        <property type="entry name" value="Diphthine synthase putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1010.10:FF:000004">
        <property type="entry name" value="Putative diphthine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004551">
        <property type="entry name" value="Dphthn_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00522">
        <property type="entry name" value="dph5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882:SF0">
        <property type="entry name" value="DIPHTHINE METHYL ESTER SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882">
        <property type="entry name" value="DIPHTHINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036432">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="9">
        <location>
            <begin position="3"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8175665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="278" mass="31651" checksum="B5AB2FAB357B7C06" modified="2022-10-12" version="1">MVFYIIGLGLGDHQDITIKGLEAVKTCKEIYLESYTSILGINKQKLQDFYGKEVIEADRECCETGIDRILENVSTDPQNNYAFLVVGDPFCATTHTDLFLRAIKLGIKVEVIHNASIINAIGCTGLQVYRFGETVSVPFFTEKWRPYSFYPKIKANLDHNLHTLVLLDIKVKEISEENLARGKKIYEAPRFMSTQIAVEQIIESDKQLGLNAIDEKTKCFGVARVGFESQKIISGFLHEFLEIDMGAPLHSFVICAKELHPIEEEMFQFYRNNQQKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VH48</accession>
    <name>A0A8S1VH48_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sphingolipid delta4-desaturase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0640134</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8176031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VH48"/>
    <dbReference type="OrthoDB" id="200948at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042284">
        <property type="term" value="F:sphingolipid delta-4 desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013866">
        <property type="entry name" value="Sphingolipid_d4-desaturase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12879">
        <property type="entry name" value="SPHINGOLIPID DELTA 4 DESATURASE/C-4 HYDROXYLASE PROTEIN DES2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12879:SF8">
        <property type="entry name" value="SPHINGOLIPID DELTA(4)-DESATURASE DES1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08557">
        <property type="entry name" value="Lipid_DES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01269">
        <property type="entry name" value="Lipid_DES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="277"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="328"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="Sphingolipid delta4-desaturase N-terminal" evidence="2">
        <location>
            <begin position="42"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8176031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="359" mass="41946" checksum="B51362B05F0982F0" modified="2022-10-12" version="1">MFLNKQGLLEDTNQPELLKEQVQKNGHTSTKDSIFSNTKFEKNMTYFHIMGHDEPHAIRRRQILEKHPEIKILFGPDLMSAWLGVGIVLAQFWCINFFATTNWLWYLVSSYCIGAVLSHALHALMHDFTHYLCFESVQYNKLMAIFLNFGQGVPSAITFGRYHSDHHTFMNLPDLDPDLPSQWEVDHVKGPLRKFLFMLFLPLFYSLRPMFIRPLIPNKYEILNLVSIIVSNTLIYRYMGPSALGWLILSTYFGLSVHPLAAHLITEHYEFINRLETYNYLGIANFLILNLGYHTEHHDFPNIPWSRLPLVKKIAPEFYDSLPYHTNYTLAILAYIFDGYMGPFSRIVRKELKYNGKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VHH3</accession>
    <name>A0A8S1VHH3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0650191</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000065">
        <property type="protein sequence ID" value="CAD8176654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VHH3"/>
    <dbReference type="OrthoDB" id="303340at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="103"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8176654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="252" mass="29726" checksum="268E17256E3B7DDC" modified="2022-10-12" version="1">MNSHVYLTKHQIHEMFVDSTQALASPSFVEPNSPQAKEKRYFFHEEVVSPSKKDDKSTDNYSNRKISQDSIFDSKVEMKLTNNQICLVNLFEQQNKDKDRETITKLQQQLSQQSQLILSLQNQINILKDLNKKNQSNISELLGQIRKLEQENQFFQDQLLKNRLSLQDQIKQQQQIKIDMENTKIQLLNKDQMIESLHQQLQSFRQDQSRCRVGRSPNRIIEQIVNCHHPSISYINYRIAPQRSHSSNIKNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VNL2</accession>
    <name>A0A8S1VNL2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0680104</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000068">
        <property type="protein sequence ID" value="CAD8177815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VNL2"/>
    <dbReference type="OrthoDB" id="272141at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14137">
        <property type="entry name" value="STKc_GSK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050591">
        <property type="entry name" value="GSK-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039192">
        <property type="entry name" value="STKc_GSK3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057">
        <property type="entry name" value="GLYCOGEN SYNTHASE KINASE-3 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057:SF0">
        <property type="entry name" value="PROTEIN KINASE SHAGGY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="11"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8177815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="307" mass="35808" checksum="6254930E10E0FF9E" modified="2022-10-12" version="1">MSFPHQKLPSFDIIRVAGSGSFGYVFEAYDHNRKQKVALKRIEKVGNQLSREYEILFEVKECEHIVKILDFFYSRTDSGKLIQNIVFEYMDDNLENRIQIFIKQGKTFSELTIKSYIYQILKGLQFIHKKGIAHRDLKPENVLINNNEVVKLCDFGSSKLINSQGQNTPYIVSRYYRAPELILCLTKYGVSVDIWALGCIMGELVIKEALFKGKSEGDQLFAILKVMGSFNSNDMEYFINKVPFDNKIIFKELSRYKKQNLRDKFSQIKDLDNYLDLLNQMLQYNPEERISAKDALKHPFFKDVANE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VPF0</accession>
    <name>A0A8S1VPF0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">cathepsin L</fullName>
            <ecNumber evidence="10">3.4.22.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPENT_87.1.T0700166</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="11">May be involved in extracellular digestion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Specificity close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity.</text>
            <dbReference type="EC" id="3.4.22.15"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.15" evidence="10"/>
    <dbReference type="EMBL" id="CAJJDO010000070">
        <property type="protein sequence ID" value="CAD8178887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VPF0"/>
    <dbReference type="OrthoDB" id="190265at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000104">
        <property type="entry name" value="Cathepsin L 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035895208" description="cathepsin L" evidence="12">
        <location>
            <begin position="18"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="14">
        <location>
            <begin position="34"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="13">
        <location>
            <begin position="118"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8178887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="331" mass="37033" checksum="A6E722C87A9A1C17" modified="2022-10-12" version="1">MEKQLLALLTVTLLTSAAYFMNGKNENLTLEGQFQSWKQQNGKRYSNYEEIHRFSVFAQNLALVMEHNSRFELGQETFTLGMNQFADLTSEEFQASFLTLKPQISEKQNIKKFSGLKFPDTVDWKEGLTVKNQGNCGSCWAFSAAAAIEAGLHHQKKEKINISEQEFVDCTTAKLGYESEGCNGGWMDDAFDYTVNYGVATEEEYPYKGVDQTCQTGLKKKYYIDSFVDVEPLNSDALHEAIAKTPVSVAIKADGIMFQLYSGGIYSRTCTAKKIDDLNHGVLAVGYAKDSYTIKNSWGAGWGEKGYMRLAIIAAKEGQCGIHWVPSYPVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VU04</accession>
    <name>A0A8S1VU04_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0720126</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000072">
        <property type="protein sequence ID" value="CAD8179605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VU04"/>
    <dbReference type="OrthoDB" id="292842at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8179605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="78" mass="8432" checksum="2DD6F2764C38B2B9" modified="2022-10-12" version="1">MGASCGSMNSSIIRRDSGNGNMGSNIIFVQAPFDPNLKIDDIGLKNVLTDFDNLIAALQQLQGEVLKIPSKINEIQIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VXT5</accession>
    <name>A0A8S1VXT5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0740250</name>
        <name evidence="2" type="ORF">PPENT_87.1.T0740252</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000074">
        <property type="protein sequence ID" value="CAD8180688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000074">
        <property type="protein sequence ID" value="CAD8180692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VXT5"/>
    <dbReference type="OrthoDB" id="319771at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8180688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8180692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="200" mass="23998" checksum="C3AD999349E0D273" modified="2022-10-12" version="1">MINKLFNADSQLKFNPIQIAVNTQSLSSTLFIININNVIIISICYSNMPIPISIIEVKFKIKQINIINQQLGLSHICNLWFDLCFQVWSIQNFKQPFFMRNMISVDYKNYLQILSDNLFYYVQFINYTLYVYNPYLPQHMSLYYLNLSSKLYCTDFRKEIEIQSKNQRGFFLYLENFINQLLIYQSFEFKVNQSLDFTHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VY52</accession>
    <name>A0A8S1VY52_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0790073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000079">
        <property type="protein sequence ID" value="CAD8182684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VY52"/>
    <dbReference type="OrthoDB" id="406844at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="209"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="253"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="296"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8182684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="468" mass="55139" checksum="FE2A333401F6D509" modified="2022-10-12" version="1">MNQKLLCPSHNELIQIVCFNEKCQSFRFGCYECLKKEHHLSHTRDLKTIQEFYEYKQLKQKEYLDFSLTFENYFDKTMNLFNQIRNKLSFFQKSKIDTLDQMNDVLSLFLQNQKNQDKFSSIMMDIQKKLISVLQNFNENLIESQIDSSQQHPIKKETKYQVIQNIRESLIYSFSFNHNSSMFAAGYSTGQIAVFELEVEQIRKQQQMLNGHKGSVYSLEFFNYKDCFLSGGQDRMILIWSFQNKIWICQQKIDAHSDWISHIIINKNNNLIISSSGDKTIQFWNKDEQWKCCQTITAHLSTVCSLSLNETEDQLISCSCDSTIKIFEFKNSNWIILQTINVEKLGFRLCFINNFTFVFQSENSLDLSVYKFNQQAGQYHLTKNVQVQNGENCSNLFPQKFIKSKNIILNKNGSKVNLIKISPDEEFQLIDVIDFSTRKIFGNMSNDGQYLCTWDWISKQIQIRQCIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W2S8</accession>
    <name>A0A8S1W2S8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0800010</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8182967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W2S8"/>
    <dbReference type="OrthoDB" id="194865at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051723">
        <property type="term" value="F:protein methylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016812">
        <property type="entry name" value="PPase_methylesterase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14189:SF0">
        <property type="entry name" value="PROTEIN PHOSPHATASE METHYLESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14189">
        <property type="entry name" value="PROTEIN PHOSPHATASE METHYLESTERASE-1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0719">Serine esterase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="4">
        <location>
            <begin position="122"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="26"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8182967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="413" mass="47063" checksum="FA5C4B5C2AF83947" modified="2022-10-12" version="1">MSDLLKQTLKAQQKQKPPVPKKQMQNEDEDEGDDKDEEQDQFDVDFNKNSIPVKLQGSKPQPIKADHNLTHSHHQNPQQAKITKKIHDYSPQKWNDFYDEMIFHPNGTPIYIAGKNKAPIFLCLHGAGHSAMSFANLANEVKQYATLISFDFRGHGQSKIELENPNLSVQQLLDDVIEIFDYVTTQWPKQTVIIVGHSMGGAIAAKSANLLITSQKADKVQGLIVIDVVEGSAIEALPFMEQIVNNRPKHFKSYEQAIQWSLNTSTLMTLSSARVSIPAQLKEVQDQNGNLISLDWKVDLLKTSPYWMGWFEGLTDSFLQIRIPKILMLAEKERLDKDLTVAQMQGKFRLIVLQNTGHSIQEDDPKSTAYNFHDFILKFRIPTTVEEVDKLKQVGIGKFHPTIGNYEYQQKYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W5S6</accession>
    <name>A0A8S1W5S6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0810155</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000081">
        <property type="protein sequence ID" value="CAD8183745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W5S6"/>
    <dbReference type="OrthoDB" id="293479at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8183745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="267" mass="30957" checksum="4C71C9D131B48EFA" modified="2022-10-12" version="1">MQDKSNTNSSVQESYSPKKPADGGEKKKRSRTRKYSDAQREHKCECGKSYLSYPALYTHIKQKHNGQSLKNLDEQRLESTFSESQTQDIFATLANALGKDEDKPQASLNIQDMYDLIMWEVQLQIMETENKEIKFDYYHELIKSTQPNDVTTPQDAFIKFIHEVKGVLSFDQMKFIFLFVYSFRKLLLDCPECPTSGLVEKANQLICSQLDKVLQSIQQLSNRNYIVPLCRSANLREIMIQIVLGFCNWLYARHLTDDKLLLKSDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W7M1</accession>
    <name>A0A8S1W7M1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0850046</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W7M1"/>
    <dbReference type="OrthoDB" id="283158at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46364">
        <property type="entry name" value="OS08G0421900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BAH" evidence="6">
        <location>
            <begin position="62"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="5">
        <location>
            <begin position="184"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8185361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="234" mass="27630" checksum="462979ED35DA611B" modified="2022-10-12" version="1">MSASLEPKISRTSSLDDKQDNVLQQSKIENLIQKKDNNNLYKLLKKNKKSRTSYKKLKYDGIIYKIGQNLCIKADRRVDYVAKLIKIVKLVDNNDEIYPLIKVQWYYRKFELGDLPMTYMDYISENEVFKTNEYDYIEIESIVSLASILTYQEFDKLETMNDTTYFMRAAYINRTFQPPIEEWATTCICQKPPNPDLKYIQCEACQGWCHLKCVDLTKEKAKKLLNFVCPKCQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W9M2</accession>
    <name>A0A8S1W9M2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0850122</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the DP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W9M2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004345">
        <property type="entry name" value="TB2_DP1_HVA22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300">
        <property type="entry name" value="HVA22-LIKE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300:SF161">
        <property type="entry name" value="RECEPTOR EXPRESSION-ENHANCING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03134">
        <property type="entry name" value="TB2_DP1_HVA22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8185513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="93" mass="11863" checksum="A0AB6589D4AAE83D" modified="2022-10-12" version="1">MTLEIIETQNYKNTKQWLSYWVIVMIVHLLDDYFYWILYYLPYYHFMKFIFFVLLFHPKTQFAEHFYDAVFHKCYVNYQKYIYSSKRNPSFNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W9W3</accession>
    <name>A0A8S1W9W3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0850246</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W9W3"/>
    <dbReference type="OrthoDB" id="5337378at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001582">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001156">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF190">
        <property type="entry name" value="MITOSIS INHIBITOR PROTEIN KINASE MIK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0652">Protein synthesis inhibitor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="135"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="78"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8185737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="418" mass="49287" checksum="D134CEDC07CD478F" modified="2022-10-12" version="1">MNVFPSRNLFSNPSSPQTLEPNPCKKLFVNPPFTPEFGNQIKQPIKLQVTEGIRINQKNQLAMKIQQQKSLTYQLNQISSPSTPKHKEKKLQQSTGEKSKEIQQNKMNKKKVIRNLNKQFEGELEVPKSRYQEEYIQVDILGSGYFGQVYKCKNRFTNQICAIKCTKINNNKLNIDLANESQALAYLNAKGACKNLVRYFTSWQEGNMLYLLMEYCDYSVSSRQDYEEFEIKKILKDITNGLIFLHEQQISHLDIKPENILYSKKDGQYKLADLGLSKKIQKRQEDVNVGDFRYIAKELLNQTEKLDLCKTDIFSLGATLYQLMTRKQLPSNGEEWRRIREGIQINDFPENMYSLRLRKLICRMMDPNPYQRISAKEILEDEYIYVKKENYIKWEKIRGLMLRRQLDEFLIKQREHSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WF25</accession>
    <name>A0A8S1WF25_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0880197</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8187200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WF25"/>
    <dbReference type="OrthoDB" id="406368at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051291">
        <property type="entry name" value="CIMAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580">
        <property type="entry name" value="SHIPPO-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580:SF60">
        <property type="entry name" value="SPERM-TAIL PG-RICH REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8187200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="389" mass="43951" checksum="CC3D8633A9C3944F" modified="2022-10-12" version="1">MNYPFNPSPSYSFSVAKKIQSHFLLPQISMGPGRYNPKTERKGGTLNFGSYSQRKMVLAQDTLTPGPLHYTINTHRSQGVSFANSKKTLQKPFRSPGPGQYLSIGEPHSRLGGSISRTSRNNNFDNQIPGAGHYPIKSTLNSLGFKFKQLHKRAKTDHMSPGPGSYSILQDSMNHGVKMHNSKRTWSYILKTETPGPGTYQSESFIQNRVSSKHSKTSSKEQSKILNKCFSQHESKQQSNKLGTFSQSKGNLDSHGSLTSRQKFKSNHITQQQNISDAYNRMREYGKLKVTFTKQVRKEEFLDEFSSSNPKKISRSPGPAIYADIKFKPNTKLGFVPTSKKGEVFKPKEGPGVGEYSKIIPFGKVYNKHITPKRQFFKFKDLNQFDFDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WIK2</accession>
    <name>A0A8S1WIK2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0920088</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000092">
        <property type="protein sequence ID" value="CAD8188781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WIK2"/>
    <dbReference type="OrthoDB" id="268414at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8188781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="93" mass="11417" checksum="D3CC4EEF46F9D327" modified="2022-10-12" version="1">MSKQPEYILDSYKVPYYLQDSCVDEYVYYQQCLRHNPRFFENKLIHSLPFASALSNCAKKQQIFVRCQEYRERELFEEMRKIYVDSVRKGEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WNB4</accession>
    <name>A0A8S1WNB4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0990067</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lin-54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000099">
        <property type="protein sequence ID" value="CAD8191494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WNB4"/>
    <dbReference type="OrthoDB" id="6283463at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005172">
        <property type="entry name" value="CRC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028307">
        <property type="entry name" value="Lin-54_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446:SF34">
        <property type="entry name" value="PROTEIN LIN-54 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446">
        <property type="entry name" value="TESMIN/TSO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03638">
        <property type="entry name" value="TCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51634">
        <property type="entry name" value="CRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRC" evidence="4">
        <location>
            <begin position="70"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8191494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="188" mass="21224" checksum="C74627D48D8927FB" modified="2022-10-12" version="1">MAAENCNSFVRFALNLIQTEDIFGITSPKRESPIPILKFFDLDESPKQNSGKKPFPIQQKIVKAKQIEKCIIKCKCLKSKCQKSYCECFAAGRSCNIECNCLGCNNSNCSQQIQKTQCGGCNCKKTGCTKKYCECYLKKQRCTFLCNCIDCCNQEDSESEKENEQNHCIGILSSNLLMNSQITEQLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WP43</accession>
    <name>A0A8S1WP43_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WD-40 repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0960001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000096">
        <property type="protein sequence ID" value="CAD8190225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WP43"/>
    <dbReference type="OrthoDB" id="7875889at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035097">
        <property type="term" value="C:histone methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000228">
        <property type="entry name" value="COMPASS-like H3K4 histone methylase component WDR5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002141">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF8">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25173">
        <property type="entry name" value="Beta-prop_WDR3_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="216"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="258"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="300"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="342"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="384"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="426"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="468"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="551"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="593"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8190225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="712" mass="79599" checksum="F1F236ECB83DACE3" modified="2022-10-12" version="1">MYWNIQWKETLLDILAYVKDIDEQIFGDILEILRKEKVIDCIGYLSGEENLSHLQQSFLQQGENMTHEKKLYDINKYMKLISDVLNKLKNHNFNKKDYSQEKNYTYIKFLITRIKVYSQIIKFLQFLVHLTSIDNSVIQCGSNSLYLLVQMKVDLRSKNFENIKIQNTSLEGSNFFRCNFSGSLFDNVYIGGLNLNGAVLLNCNWKNLRIPELNQLDGHSDQVRSVCFSPDGNTLASGSYDKSIRLWDVKTGQQKAKLDGHTSGILSVCFSPDGNTLASGSVDKSIRLWDVKTGQQKAKLDGHTHYVYSVCFSPDGNTLASGSYDNSIRLWDVKTGQQKTKLDGHDHWVTSVCFSPDGNTLASGSGDKSIRLWDVKTGQQKAKLEGYYGRINSVCFSPDGTTLASGSEDKSIRLWDIKTEQQKAKLDGHTRGILSVCFSPDGNTLASGGFDNSIRLWDVKTGQQKAQLYGQYDCVYSVCFSPDGNTLASGVWDFSIRLWDVKTGQQKAKLDGYHSYVKSVCFSPDGNTLASCSDNSICLRDVKTGQQKAKLDGHSYEVYSVCFSPDGNTLASGSVDKSIRLWDVKTGQQKAKLDGHTSIVYSVCLSPDGNTLASGSDDKSIHLWDVKTGLEIKSSDKNYKDLLALFKIPLQQHSHISEASNYITTLLISQQTIFQAQGALILKGDFIIHSGIDLKTLFKKKGSCFLEDLQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WQR4</accession>
    <name>A0A8S1WQR4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">rRNA 2'-O-methyltransferase fibrillarin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Histone-glutamine methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPENT_87.1.T0990106</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="14">S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ105me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-glutaminyl-[histone H2A] + S-adenosyl-L-methionine = N(5)-methyl-L-glutaminyl-[histone H2A] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50904"/>
            <dbReference type="Rhea" id="RHEA-COMP:12837"/>
            <dbReference type="Rhea" id="RHEA-COMP:12839"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61891"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. Fibrillarin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000099">
        <property type="protein sequence ID" value="CAD8191572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WQR4"/>
    <dbReference type="OrthoDB" id="298980at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990259">
        <property type="term" value="F:histone H2AQ104 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008649">
        <property type="term" value="F:rRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000494">
        <property type="term" value="P:box C/D sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000001">
        <property type="entry name" value="Fibrillarin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000386">
        <property type="entry name" value="Fibrillarin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00351">
        <property type="entry name" value="RNA_methyltransf_FlpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000692">
        <property type="entry name" value="Fibrillarin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020813">
        <property type="entry name" value="Fibrillarin_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003276">
        <property type="entry name" value="PRK04266.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335:SF17">
        <property type="entry name" value="FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335">
        <property type="entry name" value="RRNA 2-O-METHYLTRANSFERASE FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01269">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006540">
        <property type="entry name" value="Nop17p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01206">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00566">
        <property type="entry name" value="FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="25"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8191572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="278" mass="30127" checksum="4A8550A3FA869F60" modified="2022-10-12" version="1">MGRDFRGGRGDTRGGGRGGDRGRGAPRGRGAPRGGRGGRMGGAPKAFVVPHPRLAGVFVAKGQQEALVTKNMVPGESVYNEKRISVEDKQTGEKVEYRVWNPFRSKIAAGVIGGVSDIFIKPGAKVLYLGAASGTTVSHVSDIIGSEGVVYAVEFSHRSGRDLVNMAKKRTNIVPIIADARKPLEYRMLVGMVDVVFADVAQPDQARIVGLNSQYFLKKGGHFMISIKANCIDSTNRAEVVFQHEVQRLKDEGLTPQEQLTLEPYERDHAIVIGVYNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WS18</accession>
    <name>A0A8S1WS18_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1020018</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000102">
        <property type="protein sequence ID" value="CAD8192523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WS18"/>
    <dbReference type="OrthoDB" id="307517at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="91"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="136"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8192523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="366" mass="43589" checksum="2809DEF9B7CD0DA9" modified="2022-10-12" version="1">MAKLYHNLNQINRDKVDFRELKQKFININYSNISINGEIQLKLINQSVKQSGRCNAIVFDSSGSIMVSTEKNYIIKVWKFQNGKIKLITNLQGHTRHIQCLVYSKKQNSFISGAGDKTIRCWQQVDQFNWISSQAYQQHKDYVRCIILNQNEDLLFSGSTDRSIKVWKINLNKNKLKYLYSLYKHDDIVNALNLNQSETQLVSCAYNKYQIIIWENGEYNKFEFKYIIKQSIQDYGFKVQFIKDNQFIWLTGGQEIDKLFVFELIDGIFQENQDKTIQLLKNNSTIDEYRFPILYNKEMNLIVVRHKTYIYIIRQTNYEKLKIVDFLNCYTDDIYGMITNNAKYLVYWDKKNQAYSTYELLNKQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WTG5</accession>
    <name>A0A8S1WTG5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0990041</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000099">
        <property type="protein sequence ID" value="CAD8191459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WTG5"/>
    <dbReference type="OrthoDB" id="5337378at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="13"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8191459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="521" mass="61122" checksum="0DF1F048C746A773" modified="2022-10-12" version="1">MSKSLIKIGKYTLNKKEMIGKGSYGLVYQAIYDGEEFCAKQILTNGPQKKFTDRELQILRMLRDSPIQNPNIVHIFDVIQNEGDNEVYIIMEYCRGGDLKKEMEYKKKHNQWYTHQESLNIIAQIIKGYKSLYEVEFIHRDLKPANILIQSGFYKLADFGMGRFINSTQSSNVGTPAYSAPQIFFDPYYTNKCDVYSLGVIFYELIFKEKPINGKTQAEQIPLLRSSKQNKITCPRDENIDLRICVLIEQMLSYEEQNRISWIDLFEHPLFNGQQALQTAHIRKSSLLVQKQQVKFQKRPNTPYYHFSKKLYNSLEDAQNLNGLKHLAEIIMSKGDVAYDLAIALAEQQSIIAENQLQILLFCLFSYKYFACSNTMGMCTNQDNYCHNSIFQIFPKFHDLIYDYSKSNTQDSQQMRSIIVSQFGHLNGYYRDSQQFVSQINELENQHPEFYSKVVERDNDQNNYISYLYWFVFFFNKWFEDTVFPNQNQINKVLTLESAFPLEKFWAKSPSQIITIRKNSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WU70</accession>
    <name>A0A8S1WU70_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0930136</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000093">
        <property type="protein sequence ID" value="CAD8189306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WU70"/>
    <dbReference type="OrthoDB" id="291919at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8189306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="299" mass="35339" checksum="8AB29F77E6F803BD" modified="2022-10-12" version="1">MKERVSDIQNIYNNSMAIKQIKYREKLRQIQETDIMSSLYQFRKLTQQDLAQFQEGAKQIFKIIAPGFDTRFSEITLTSTSKVFIELYQHFGFVQKSDTLYNELLHNSKQQIFLCLIQHLIDLTKIALKCAVKRFLDMKLFCIGKEIEKSPILLNKRIDMIQFTAVKKNKIKRVMRSESNSLHQSDKKQELRIIIDKKDRLSIHDVISHCDKSEIIKKNIRNYDEINIDNISTTCNRNIQRYQSVQKHTEPTTQRLQFPSIYKNSNSVLYKALKTRNQSISLTISNYIRELQDKGIYEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WUW5</accession>
    <name>A0A8S1WUW5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1020022</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000102">
        <property type="protein sequence ID" value="CAD8192531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WUW5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8192531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="121" mass="14142" checksum="0A717FBFE2467CAE" modified="2022-10-12" version="1">MQQMHIQIGLNKLKTNLNDNQQEQADKIAFNKETVIGLFQALLMAQSDNQHHRFYAQIFSLSKNLQIAIEKGLIRNDWKFRLNIIGPNILYNQITPVQLMSQEINEACLIDLNLKFYIKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WXL5</accession>
    <name>A0A8S1WXL5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1060060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WXL5"/>
    <dbReference type="OrthoDB" id="446723at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008536">
        <property type="entry name" value="DUF818"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF81">
        <property type="entry name" value="PROTEIN ABHD13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05677">
        <property type="entry name" value="DUF818"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8194172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="378" mass="43545" checksum="A56D4D95D0FD98C9" modified="2022-10-12" version="1">MQHFVTGILQSFGLCGLRNKLVHFVAFRPPEASYELRLRKSNNHKSHTKQKYISSDDNQISDQSQNNQSQTHRKTQSQYIQRRRRYIRNSEEYPEYDFIKLSSDKSKDQNEDFIVSSDICSVTAYFLNQKKNKQMACVYLNRNSDQIILFSHGNACDLGMMIDKLIKLVQYTNTSVFAYEYSGYGQSDGVSNDINIIRNIYTAYNFLIHQLGYKATQIIVYGYSIGSGPSVTLASNPQYPIGGLIIQSGFSSGLRVISNKIDETPFYDMFPNIDRIQLVSCPVFIMHGANDKIISDEHARQLASKTNNLYELWIPENVGHCGIETDIQNRQQYFQKLQKFIQHIQMLNESIPDLLKKNTALIQGKIPCKSHFYQTKIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WXS7</accession>
    <name>A0A8S1WXS7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1060091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WXS7"/>
    <dbReference type="OrthoDB" id="293833at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8194232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="76" mass="8925" checksum="2965FEA94914CDAF" modified="2022-10-12" version="1">MEKINEQLPDISSITGIMFYSFISYWMIFALSIIVDRFMFSSKSNENSKMKKIIKARDVKKQQADKLLKKIDNVTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X3P3</accession>
    <name>A0A8S1X3P3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1100003</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000110">
        <property type="protein sequence ID" value="CAD8195547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X3P3"/>
    <dbReference type="OrthoDB" id="203073at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF263">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="147"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="149"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8195547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="171" mass="19280" checksum="DE93FE939F0F9B97" modified="2022-10-12" version="1">MGSCCSSNKVIKETYDYSDGGVYSGDLMNGLPQGKGQIRWENGCFFEGLLQEGRKNGRGLFRWADGSYYDGDFTDEKFNGYGEYYWADGKVYKGNWVDGDLNGEGILHYKDGRIYKGNFTNDKRDGQGEMIWPSGSKYIGGWKKGRQHGKGELIDTNGKKTEGIWNKGKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X4Z3</accession>
    <name>A0A8S1X4Z3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1140094</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000114">
        <property type="protein sequence ID" value="CAD8197063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X4Z3"/>
    <dbReference type="OrthoDB" id="311196at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="371"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="422"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="120"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="230"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="435"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8197063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="445" mass="51974" checksum="44091D8836CCD83E" modified="2022-10-12" version="1">MNTLSSNPKKTMFYAAAQNMLNSNQSSMQNLKRSLFQLAMFPSSHQKDQLNLSLNNNSDRAQANYSLPRRSNLFQNDRDRSLSYKQNISMEQNGSVERLQNAYNIKGHFQLMNEDPNQIVEKEQKKLELKEYLLKQIEEKKNQQKYEQERKRIEDAKAEQLVQKQLEEMRVRQHSKFENQNMQQSQASSIQTKAIQQLSVSPQKEGIFMQIYKQKQQNIVSQQTDILEKINHLQNDIKSLQINQQQQNDVNSVRNNIKLMMKKQSSMNPEQINKIQKKDIQQLKERQLDCETIFIPANLKLNTDVDILQQNDNTIQDVNKSTNSLNFTKNSQNQLLLNNTSIISNQVINNSQQQVNNQIVNHSRLTISQLSSRNRSLSNEKQVLPDPIQSDNTQEETKLIQKQKKPKQNNLQLKLPHRTTMLMQIPSKNQISTQKSEEPKSSQRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X612</accession>
    <name>A0A8S1X612_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WD domain, G-beta repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1050146</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000105">
        <property type="protein sequence ID" value="CAD8193986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X612"/>
    <dbReference type="OrthoDB" id="307592at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8193986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="490" mass="57492" checksum="C7453D5BCDE53940" modified="2022-10-12" version="1">MQNSQYEIIIEYIKSLLCPDHNLPIKYINYSTDVKIGNRLICSQCGKNGLDGYFEIEAAIHIINQPKISQPITNACLQLSNYIDGIKTKVDSLTNQQIYFLKNTQNRQGITKFQEETQNYSLSNHNEKKTEKIIKQGKDAIENSIRYLERFQMQMLSLLTHQLNQFKNFTEIHKQEYQQNIFVETSIQDYEFFIYSSKFLEVFTTQDNQINSIQTIESPKTKITCIIKAQQKQMFFLGRQDGIVEVYEQLTNGKYISSHQIQAHSYNPSQNYNGVTCIITNQDNSEIITGGQDTLIKQWKFRKNKWNLISQLSEHTGVINSLCYSLDFNILGSTSQDYSFSLFKSSQDVYQKSIQEIKQSRVNTICFVTSTKFFISLNQDRNIYFYQIINPLQQQYQIEQLYNISLNEINTDIHINVQPRFYQQQNLLIFQSNSQTYLISDKDSNKFKIILIEKQNSLKGNIFRLLRFGQLAVQVYQKNLKYFLSLTQFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XB33</accession>
    <name>A0A8S1XB33_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1180038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000118">
        <property type="protein sequence ID" value="CAD8198260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XB33"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8198260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="53" mass="6123" checksum="976F5DB7F9021F06" modified="2022-10-12" version="1">MLKIIGIKQQYLGEPQLQLNEFRGKFWNSKGYNNPKAQKPNKADLFKGAQQQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XEH1</accession>
    <name>A0A8S1XEH1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">YTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1220013</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000122">
        <property type="protein sequence ID" value="CAD8199530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XEH1"/>
    <dbReference type="OrthoDB" id="306690at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061157">
        <property type="term" value="P:mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21134">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007275">
        <property type="entry name" value="YTH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045168">
        <property type="entry name" value="YTH_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357:SF89">
        <property type="entry name" value="YTH DOMAIN-CONTAINING FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357">
        <property type="entry name" value="YTH YT521-B HOMOLOGY DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04146">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50882">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YTH" evidence="2">
        <location>
            <begin position="44"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="304"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="304"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="327"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="378"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="396"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8199530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="407" mass="49072" checksum="D60E180DB6AEEAD7" modified="2022-10-12" version="1">MQATSDQQAFVSLENYDDIDLIRSKFPPLANINIDHNLNNLKDCRFFIIRTQGEDNVHRAMKYGIWTSSSRKNERLDEAFRNKKEDVYLFFTEINSMCFSGMAKLTSEFNAKAHFKYWLIENKWFGTFQIQWLYVKDIPFKHFDEIKQIQKLEGSEETLKSVYDLIDCTELTVDNGIKMARIFQMEKTNKSLFEEFPQLDKLEFQSRQERTNNIRFEKKFQELSAVFETIPFSFSVASYERKKTNRKAQGSYYQSQYGQQYYYQQPYQQYYQQPFVGYQFQQTPQYWQFNNGFYQNQYQYQQNQYNNNNNNEQGFNKPQQSYEKQQHLQNHYSNQQQQSNTVSQQPQQNTNQESNQNVQSVPLEQRFQINHQQLQKKGKFDKNRNKNNKDNIQYVEKNSTEAPVQKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XJQ1</accession>
    <name>A0A8S1XJQ1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1280146</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000128">
        <property type="protein sequence ID" value="CAD8201700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XJQ1"/>
    <dbReference type="OrthoDB" id="291100at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8201700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="211" mass="24845" checksum="E5DA09E62B89F68A" modified="2022-10-12" version="1">MINFQKGVQDSQLKSTIDYRINRQFQVVSKSREIRQTQSQCQNKVAINNNNYIKPKRSIQYNSIQTDSTAFESLETDQRRQSTEPRNYQKRRSRIISTKIQSQIDLLQSCDMDHLESEQIPIGNFQIQQQQYSEPPKNCFINKENYQGRRTKLPTLKKHQLQFEKNEGKISGSNVVKFGESFVTPFFGTKKPISLEKRPNEFQGLVYKRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XMQ2</accession>
    <name>A0A8S1XMQ2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1280159</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000128">
        <property type="protein sequence ID" value="CAD8201722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XMQ2"/>
    <dbReference type="OrthoDB" id="273771at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="402"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="444"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="149"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8201722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="579" mass="67623" checksum="696B1F512FA23FE7" modified="2022-10-12" version="1">MNCTYHIKSPISLICIAPHKCQRKLCVECQNDHGVDVKQIVPINKFQEMAIQKLRDSKLDDTSELTKQRMAINQMLSFTETQLKKILEELSQSIKQIYDMIEQQTKSYLNIISKNTNLFETPDTELEKLAQILEGKTINVWNAQKQFYFNQLEQVKNLWGQEIKSLNEKIKQQMNEIFSKQFKAYSTIQPIQEEGQVYQMKEDLFELLTQIQDIDETFYKKILEILRREKVSDILEFLSKENNQHFDESLINKQENISELGKKIMKIINVLKNIQDHKFNKDDFSLETYEKASQDLIKKLSKNYGIINFIKFLVLLTSIDVNFIQCGSNALNFLIRLNLDIRKQSFENIRIKNTSLIGGNFARCNLNGSKFDNVDISGLNLNGAQMFNCQWKNIKIHELNKLDGHRSCVLSVCFSPDGQTLASGSQDNSIRLWDVKTGQQKAQLDGHNDCINSVCFSPDGNTLASGSEDKSILLLYQKQVFFRLQIYRNLGQKCKISFFQHTFAEKLYIYNLDTSNIMVNPDFNFSRFKRDFYKKPFPFRRIIGVNYNFLQQHLINLKTNLYQPLIQIYFNIVLVSNIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XMU3</accession>
    <name>A0A8S1XMU3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1310066</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000131">
        <property type="protein sequence ID" value="CAD8202495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XMU3"/>
    <dbReference type="OrthoDB" id="294314at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8202495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="84" mass="9906" checksum="DD8CDB2FEB480262" modified="2022-10-12" version="1">MDSFNLLNNKFMQQAIERKSLRSINLSQKNLDTQSKSIEYDLWDRNEGLDSPMTPKIQIPNQFYPKLINTVRLSNKRLSCMDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XQX8</accession>
    <name>A0A8S1XQX8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1350086</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000135">
        <property type="protein sequence ID" value="CAD8203896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="306714at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332:SF175">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035897272" description="EGF-like domain-containing protein" evidence="3">
        <location>
            <begin position="17"/>
            <end position="3036"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2788"/>
            <end position="2806"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2876"/>
            <end position="2896"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2902"/>
            <end position="2921"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2933"/>
            <end position="2958"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2970"/>
            <end position="2997"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="265"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="412"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="463"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="569"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="622"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="666"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="811"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="872"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="920"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="967"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="1019"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="1063"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="1209"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="1306"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="1346"/>
            <end position="1393"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="1401"/>
            <end position="1441"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="1446"/>
            <end position="1490"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="1498"/>
            <end position="1540"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="1548"/>
            <end position="1585"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="1590"/>
            <end position="1628"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="1636"/>
            <end position="1673"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2484"/>
            <end position="2511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8203896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="3036" mass="347240" checksum="75F339D04D756FEE" modified="2022-10-12" version="1">MMFLIPLILLFQPSFSFRSFGANFTKANSNFSCPQYHYYQPDLIPHTCVGYISICKGVTEVTIKIQDNQEVLCHPRFQAFYLMPLSSNNFIVRCISPFTTTYQYNQNGDVTYSCQQTNIGYCLLAQNYTTVLKCQYCYGYAAGPLCNTLLPGIQSGIFQCNKNLICLDGYTKSSLSDTKCSYQCKLGDASCSYEPLTECIDYCNQCRNGICSSCITGYQLVNGKCFGDPNCMKFQIVYNSNNSPTGINCLECDFGYFKKGQICQLCSTIPGLETCFLCNSETECKTCQSRYYLNDQKKCVPFDGCDVRCNTCLTTNPKFCTTCDYINFKRVSTGDGNCVCAQAQNYAEKYGSCVLCTEGFCKTCTLNFFECTSCDPARNRILVDNNCPCLQGYYETELDDKKCLKCHETCYNCDNPTKNDCTECDSLKNRYLFQGQCLCKRGYVEILSNGALLCLACHSRCEKCSKPKDATTNQYCTLCIPGQKRMLTDKFKCECMKTYGDNNGLVDICYQCYYTCGSCVGDQATNCNMCLESSYRYLTLTNECKCKDNYYEDGINNRDCKRCHYSCYNCDSNSNIDQCIQCPPSRIPDGIGSTFKCLCSDPHTFDDGQSLICSQCHYSCKTCNGYDESDCLTCDLDYRQFVFSKCECPTGYYEVGQLQCSQCHFTCQNCYDKNQDNCITCSADLNRILMGNTCQCKSGTSDYNGTQAMCDKCSYRCADCTQVDVNQCISCPEFSFRELGSNKSCSCPSNLIDIPTNPICQICHFTCSSCSGFKSTQCTQCSDLQQRQLNSLGECECKSHYYEIGSAECQICSKFCLDCYQTADSCTSCQPDRYLDNVSTCLCKTKLQGIKISTFQFSTQTSCSNCHYSCLTCDGFSSSNCTSCLDSENRIQTGSTCGCKSNYFDFGSTSCQQCSYKCETCFQREDYCLSCSPSSLRQHNNLLKLCNCPNGYYDDGINSICQKCHYSCLKCMGISTNCELCSDISKRILDVNLLTCNCIQGYYDDGVEICKQCHYSCLSCNGQGSNSCNSCLTVSTTFRVFYNKTCNCLSGYYDDGSSINCKKCQNQCLTCQTYSYQCLLCPITRHLIGQSCVCDEGYYDIGTQKCNECNSNCLSCQTTSINCTSCDSTKQRVLNIQTKTCVCQNGTIEINGICQECNASCKTCQNSISQCTSCQSMRLLINNECICIDGTYESSIDNKCHFCYKTCLTCVQQDTTCLTCSDADFRIMKPGNICACFDGYFENSLTLSCNKCDNSCKTCELIATNCTSCDEVLNYNQQFNQCVCKSGYFYNAITKLCEACNFTCKECSSQFNCIECDLNTRYFDDQNFKCLCQDGYYEVNLNQCQICNISCQTCITSSNNCTSCETLLYYRILNNNQCICQDGYYDIGISMCQQCNGVCKTCQLSAFKCYSCYQNDHIRILVNNQCICQSGYFDNGTLVCQKCSNECLTCSGSENHCTSCDINQKRIDQSIIYRCPCITGFYSDENKICQKCHIKCASCLNQNDHCLSCKFLPNNNRYTLSQNCDCKSGYYDDGIQLQCQQCNPRCKTCLNTSIYCQTCFDSLRFNPPQCDCINGYFENEQKQCQPCNHECNTCINESQNCLTCKPGRITNQCICQEGYFEAGLQACLKCDIQCNTCNVITSNCLTCKGDRINPPNCTCSQGYFDDYVNDNCQLCDSLCKTCNRYGCISCAGNRILSEEFTCDPPENSICNDITPWCSTCEVAVLNIQISDDLKSIIVIFDFPLNPQSISSQVQQNVCYQILSNTSLIKFGLNPQCKIDDIDNKILYLNFGSNPTIIEGDTIEFLPNTLQQQDCNVKLSIFILNNVKSPQNPVSPILEFNVPEIQLNPCDNNIITIKSKSNDGFRGLKNIIWTYIVYGSFGNGDLDNFVQQQTSLQLLDLNIDALILPIQSNITFTVRFSNFIGQQSQQIIKILTHSGAAPSVNFNVKKQFYSFSIINIAFTIQKINCTTQLIENSNYQISLQENARTFGSSSEIDYKTISNQKEFNIKIPSYKLTPRALYTFQLNVSDSSINYFSSQQTIIEIIPAGFLCKFNGVQPIQDYKKDLKILIECQDLDTKYLPNQDPDLTINVNCTDLSTNNFCLDMKSQKIKVNKTDYEQNILKYSVKSFTVQSWKVTAYKNGSYQTFEQIVVFLEDNFRELQMNYSMGYNMRPINSYETLNFTYLIPIRDLPLILEYSIGIIYDFEVLAILQPSCYQYQFRLFDYYRQFNKGNQINLKFLVQYINDIMPAQYNLKINLNQPPQCTISIDSKGNKALSYINVITNCLFSIDTPYKYQLRILLNEKDYVDYKSTKSDYSLILYSFQSSNKFQILLPYSQIYVIVQIMDADGSITNIEQEILIDKIDQIYFNCSTLSYQNSTFRYQIALALELFLNNNQNQICLQVANQIFSNIKQQATTYKFEDQQLAYKFIKVYTHYYLNMNYTNSTNLTQRTLQESSINRCYDNTSQKFIITNSKDSNEPQYNYSYLQTNVNKLKNNINQLIKDKFQLELQINEDSLILQESLFQQRELIIESLQISILFIDDAFSILSKTIITMDSEKILIYNIGEQLLQLIQSISDQINSQAQVDGNELNIYGQQVSWKLKRQSKTNFNKLSNIEFDFTDHFVAFIEKAQTSISYNFYHLLIDYQYQLQQQLNYSYLQIDQNKLIIVRLRNYRTMQNYVNQDNFSQKYNVQLEQLQYCEETQIYNQNDIYQPSCIQLSNTNLFHICDINAIHQNTSVQVLCNCNQFGNVFLIQIPINQTKNKNNSEISIDPIITEQKNLQIFEQPFILLQSILICFSILIYYQLYTIENKPNEQITQVYQNIESSDDLNKIEKKEHLKCYPGDIFIIKENYKYIHQFFSIFYCDDLILKKSYRFLQFFTELSFLNPMAICLITVLSDELLLKMVFFINFSIILLMRFIFKLFQAIYRFGGRCAIFIVFILILLQILSYILFIYALINNNENTGYINIQIFLISGGTIVLTYFIYDPVIIMVRILLYKIIIQSIKSKQLNPLYHFVYFFIQNYKLDEIFDKYIIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XR73</accession>
    <name>A0A8S1XR73_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1350137</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000135">
        <property type="protein sequence ID" value="CAD8203996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XR73"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8203996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="107" mass="13090" checksum="79E8C2C924815B8D" modified="2022-10-12" version="1">MRLYYKKIEMDCCFHQPVSSYYFIDDTQCHYVFFYLTKITMISEFYKVAIIFTGNIIISQVFLEQLSNFWKNHNLQINSFKFEQNGFQYSFSFNAFFNNAQLFRRNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XV19</accession>
    <name>A0A8S1XV19_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Heat shock factor-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1400126</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HSBP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000140">
        <property type="protein sequence ID" value="CAD8205386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XV19"/>
    <dbReference type="OrthoDB" id="4159489at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070370">
        <property type="term" value="P:cellular heat acclimation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.430:FF:000003">
        <property type="entry name" value="Heat shock factor binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009643">
        <property type="entry name" value="HS1-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19424">
        <property type="entry name" value="HEAT SHOCK FACTOR BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19424:SF0">
        <property type="entry name" value="HEAT SHOCK FACTOR BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06825">
        <property type="entry name" value="HSBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8205386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="69" mass="8017" checksum="932714261856603D" modified="2022-10-12" version="1">MSSEDPTTKELNNFVQTLLKQMSERFDEMQGTIVSRIDDMGKRIDDIEKSVTELMNDLGFSDEENEKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XV89</accession>
    <name>A0A8S1XV89_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1400176</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000140">
        <property type="protein sequence ID" value="CAD8205486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XV89"/>
    <dbReference type="OrthoDB" id="288826at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8205486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="88" mass="10519" checksum="3746C83C83DE804F" modified="2022-10-12" version="1">MNNDLCLLIRQVRMPMRIQIPKQKKQPIFHKKAYTIPDKIYTDKEIMELYYKKFIKVEIDDSIALEIILKDDFINCNINMKLGTLNTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XXK8</accession>
    <name>A0A8S1XXK8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1430020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XXK8"/>
    <dbReference type="OrthoDB" id="300496at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="197"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8206080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="302" mass="35662" checksum="4374D6233F87F0B0" modified="2022-10-12" version="1">MSRGAPKNQVLYDGDKRNSIRTYNDNSNNRQGFQIGWDIDQSRKGDTKIAGKVGQDQRNSYIPSYQTQTYVPVVEQNQDEDYMKQLYIQEMNKKKELAALYKQPDQYYQDTIDSYNNNVKQYSNNNPPNTYEQPQLLQQSNKYDHYQQPKVEEELYNKYKQGNSGSKQQNTGKQPVAQFNPITGVAYENAYTNQRVQESQQQQQQQQQQDDALSQFSQLQQHKVTSMKNKASNIFNTDIQETENINNNRQNSRVQNKRKELDDAQYKGYGQFYKNQIEVIPTQYREQKFINRNMIDNQIFPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XYB0</accession>
    <name>A0A8S1XYB0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1370123</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000137">
        <property type="protein sequence ID" value="CAD8204524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XYB0"/>
    <dbReference type="OrthoDB" id="296904at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8204524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="143" mass="15800" checksum="5CF85F0736C2D1A0" modified="2022-10-12" version="1">MGSSCISNQKDTVFEDLFSTQITSQERGIQNINQTIEGNFQQVKLLKESLEEGIQISNIQTIVITPRCELEGAKIVGELVKLGNQITIISKKIPESPHVSERSISKKGILKNKQDINVTQSADGCLLYPSPNRKSGKNIKFKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y224</accession>
    <name>A0A8S1Y224_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1490046</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000149">
        <property type="protein sequence ID" value="CAD8207946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y224"/>
    <dbReference type="OrthoDB" id="301916at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8207946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="141" mass="16462" checksum="7F837140E47870C5" modified="2022-10-12" version="1">MINNFIIRQLFNLVLDNIMDNQNVPNSKKASIDQNDIIKNGFNLMKKQNRKYMKVSLQTKQQLHLMVYQQGKKIKEAAKILGIKYATAKTIVFHKRQKRKARGKCGIKMCGYTKKMGNRVSYFQIISITGIVTKYSMEYMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y3G2</accession>
    <name>A0A8S1Y3G2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase M14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1430122</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the peptidase M14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y3G2"/>
    <dbReference type="OrthoDB" id="293487at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050821">
        <property type="entry name" value="Cytosolic_carboxypeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756:SF45">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE NNA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M14" evidence="5">
        <location>
            <begin position="234"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="547"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8206284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="617" mass="72201" checksum="03FFC16D7E708ADE" modified="2022-10-12" version="1">MFYNRIPKPLPILFGLPDDPKPKFIWMKLGEEQQTMIQGRLTKRTFTSNPQQHFKSSKGNISYRTSIVNSDQLQGTQSVNNSSNIIYYEDFNIDVKESICKQNYIPIKNVSELQESLKNLKYRYDANHPRDYEIFGGFEGCNVQEIYYCKHDTVKIVLKSDTNSHGFCKWFSLGLKIYAFKELCIKVNIINLRALEKNRDVHVQRLSDNHEFFVPSHYSLTNIKRKDSYFAQCSFEIKSSYESYIISFQKPYSFHQLTENSQYGQLGLSKLQYPIYYWVHKQNGPLILIVARIRPIDANTSYLAEELIKQLKQTQLNVIVVPMLNPDGVVLGNSRCNLSGKDLSSNYQFTNPNLCPEIKSLLGIENVQIAIELRMHEKPHVVSSIKFNCGLPQRKRNLEIHDISSKYQKYVKIFISQNQVNYQELASFIIKALQSIQQQNTDNISYKSETDHEDDILDNFFYNPQTKEQTTRLSQPSYQINPQIQLQELLQTINPSIKEQESVNQLRNLEWKELTHRSELSHGKIKTQITPSKEIQNSFFDKQLIKRKSNYHQHSESHSQSQSDKIVLKSPFLPSREQIFEKYSNLSRSPTNKRKTLSQAKLQTLTQYQVFKYTRQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YB51</accession>
    <name>A0A8S1YB51_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1630013</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000163">
        <property type="protein sequence ID" value="CAD8211305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YB51"/>
    <dbReference type="OrthoDB" id="5835755at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000586">
        <property type="entry name" value="Rab family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="229"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="303"/>
            <end position="1224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="252"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8211305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1262" mass="149846" checksum="D1C991F905FB9464" modified="2022-10-12" version="1">MEEQIFKIVIIGNSAVGKSSLLIRYCDDTFRDIYLSTIGVDFRFKTIKLDGQGIKLQIWDTAGQERFRNITNSYYKGAQGIVIVYDVTNAKSFEDVTKYWMAELSHYADQNVKLMLLGNKIDMATEETRQVSQEMAEQLAKEFNMQHHVVSAKTAEQVENAFINLARSIYNEKSLREMVHPQQQQLTSKKNKDEKQKHIYQLMSYTSRYSSARPQTVKSTIETKITTTTTSQVNKTADQSPQPIQRSEVRRSSQIKSSGTVINVPPPTTERKWLGKNPEVVCFLLALENDRIMRENNIQFNRIKELEGVNEQQENRIRELQTKLDSQTTEITNYKSEIEKHIIRIKQLESELNEYKSKLGNVDQSLAGRLKETERKLQDSEKELTNWKNKYNTDKTNWDNEIRRLNDLLTQKQKELDDYKNNQGKNYEQLQLQLRQYETKIREYESQISTYKSDIDRLNKLLLERNSEIDRLKNELKTRLQELDDWRSKFTNLQNQFNTMKSQYESKINDLTNTLQNRDRDLNDWKNKYAKLEGQFNNLGSLQSGNESKITDLQNRISQLQSDFDRQATQLRDRDAQIQALKEKLQRLEQDNFTLNNQLQDLQSRYNNLMKENEKLQNELRNRKNELDFMKQQLQQANDQLKRYEDQINQLNEQIMECKDQIMSLQNSKEMLERQLQDLYSKSNDNVGELKSANDQLEGKDKDIADLRDQLNQFQVDRQIMESMINTLNNQLANKNDEIQRLLAQNKLRLQQLLELQSRYAQLDYLLSDLKGVELQNKQLIARINELTQLVDDLKRKLAQQELQLLQSKSNEFKIQELENIIKELAKEIQRLNEFLNQKLSENDKLNMQIISMENEINKLKNLEPLLRELEKKLQTHLDDIARLRQLLDSRTNELNEWRLKYSKLEPIVDEKRQLEEKNRLLENRIQGLQQDIQRLTKQLRQKQLDIDQLNWILNQTEGLLRNRDDSINSLETRLHNLQRSYDNERQKTLNFEQIQSQLQQSLDKINQLETQIKGLQNDLNRAKKEVTYQQNENDQMRQQIAKSEISILDLKQNEARYKEVENRLNQSQKEVEKLTRQLNDKLQAYEELKQNNGLLDTKYKNLQQQYQELLQRLNQLEQINNLYQILKAKVLELENNNKQYKIDQDRLNSTLNQKLQELNELQNLLPQLEDKIHQLTPFEEKYSIVQPKYEQELNKSDELQKKIRDQEQHLQELEKVKKIVQDQTVILTVLFGECEGLRQQCMDKDGEIQDLRFNVAQLSDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YGD3</accession>
    <name>A0A8S1YGD3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1690052</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000169">
        <property type="protein sequence ID" value="CAD8212599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YGD3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8212599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="79" mass="9444" checksum="FB251CB929BD7BDD" modified="2022-10-12" version="1">MLYKEDLGLLIFYKGDFIPRDFPSSIVELNVPHQVQRKYYLQRSLQNFRSRNSKVVLQVCTISNKIAINLIYQKFTKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YM80</accession>
    <name>A0A8S1YM80_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PARP catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1670020</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000167">
        <property type="protein sequence ID" value="CAD8212194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YM80"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36649">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36649:SF28">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8212194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="503" mass="60119" checksum="CEA02C9C486FC796" modified="2022-10-12" version="1">MKQQQNPSKIEQRLEPIITQNWELQNILAKTLFKEQISLLGNQLDKIDQNSNLTKDQKQYQKHLYQQQLFRKLQLVVEPKSIPHINQNIEASNPELMLYVLKEQLLRDLNIEVLKSNKIKSFGLDQVIRIFSQDQSCFKIRNEQLDYDKIMEDKKLLEFHLVDFTQKLSSSLQIPIDQIEILGVSKGSFEINFLIKGKTYDEIQQLIQNIPAAQVYLHKYCNGKIEYLAYFDQARVAEEQLAKSSGIILTSDEFNPKYNMKWSNFKEKEQRGPPDHRYDYYFPRGCYGFGLDIKKHGENQDWIQMDGNPNEWRIMYHGTRQKFVNSIVKNNLMVGVNNLYANNDCQDEFGNMIKVGNGIYFSNDFNVCTRDRYASYTQVLDKQFAVIFMIRVNPKKIRQSQKMKLKNYFVVNESKDVRPYRILLHEKKRDLQFSFFFIFQMKYYSQKLKYNQLRFLSQVKYQKKLEVNKLILTCQDLILCCNFRYLYKQFYPRSQFYCIFYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RVQ9</accession>
    <name>A0A8S1RVQ9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0010013</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RVQ9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8131417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="42" mass="4877" checksum="E049099BBE368862" modified="2022-10-12" version="1">MNQTTPKSSGINYARKQKSKESTISKTLNKNLQFDNQQNNQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RWC8</accession>
    <name>A0A8S1RWC8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0010566</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RWC8"/>
    <dbReference type="OrthoDB" id="296632at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000566">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000977">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23189">
        <property type="entry name" value="RNA RECOGNITION MOTIF-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="11"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="209"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8132348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="373" mass="43402" checksum="8066FB7C6FDED727" modified="2022-10-12" version="1">MNSRDPPSKILLLIITYLPPSFPLTNDYLFETFKQYGEIKKILIFERGKTNKAFIEYYDIKHAIQARRDMMGKPITTQGGRLLIHFSRLKQLNLEVVDHTRGTQYQPHEIETQVQPQKQVQNIQKAPMFIEEENKPPLIDLTQVLKLEAVDISHASEESPTKAIMETQLKQLERILDEDYANEVARSNLTQVDEQQQVINELLAQKPSKYLKVQNIDERVTAKMLYNMFNRFGHLNALLFQKKDNIAILQFTNIDHATIAKELLNNIMFFNREIRILFHQIDNLEASNPNEEYYVGSQTKFKIVPLSKVLVFSGISDLLDIQDMVKLVGKIQEIKLEQYSIQITMLDIYEALKVISVLSEYEYKGNKINLILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RZ60</accession>
    <name>A0A8S1RZ60_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0020308</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1668162at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051425">
        <property type="entry name" value="Formin_Homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014768">
        <property type="entry name" value="GBD/FH3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725:SF1">
        <property type="entry name" value="DISHEVELLED ASSOCIATED ACTIVATOR OF MORPHOGENESIS, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725">
        <property type="entry name" value="FORMIN HOMOLOGY 2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51232">
        <property type="entry name" value="GBD_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GBD/FH3" evidence="2">
        <location>
            <begin position="39"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="FH2" evidence="3">
        <location>
            <begin position="942"/>
            <end position="1331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="793"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1357"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="818"/>
            <end position="933"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8133248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1422" mass="163344" checksum="11263A07C7E9F72C" modified="2022-10-12" version="1">MGNAISNGKLISPISIDAQIKESQLTIRFMLDQKKLMAQKNTLPKDFDQQISQLIKELKVEPKLLQQFQSTPQNFKCLILNEYKNITGKNLLQNLPNDEVNYFNQKLSNPTLQDLEQLRRYLKNLQIQDQQDKLEQFHITINLLIENLFRFHQQAQQIIEKKKPPCYLYQVQILNILEILVKIEKNQQQMLTIPDSMDIILKNLHPFHVELTTTVLEIASHLCWNTDEGYNWVLKSLNKLYEDNEIIFFVNTLKKSRNAVMIATICVFIVTLTESPTQEEERKRMRQQFISTGMADVYKMIINKIEEFEYKADELRFEVVLQQIIDHNKQKHQIQTDPNFTEEIKLPMLYQRESRKCYDISEFSNSIKIIEGQIEAFLENNKDIPNQQPQEPKKSEVKQHAKHLKSTFLAPQYLNLIQDEMGKANLTNLQRTVENIKHESKIRGSQIVTEFAEGISKYIDKLNQPSQKETENIESLQFELMKQQIDDLHEKISSLENEIKDLNTKKQSNEAFVDVLQRKIGDMEKKLKSDQTNYSQLNEQLASKNKDYRALQQENESQQKAIQQLENEVNQLKEKLNIMQLAKAQKMELEVPPQRLSHKQDNTEELKQQLDQLKQDLHQQNQKFIIQENEIKKFQQQLKEQSENLQTKEIQLTEKNKECRELQATNEKLIKKKQAKKNQLQQLKEKIQSVNYVKTQATKIQNELKTIEQYQEELIRLNNIIEEQAIILEQQKQYTSKLDEMRDKQQEQELKINQMEDENKKQLINNQTLQDKLQQLQQEKENLEKKLLNQLQNAAQSSNTFAPPPPPLPGSTQTSSFIPPPPPPPPGGSKTLPPPPPPPPPPPPPPGSKTAPPPPPPPPPGGKSAPPPPPPPPPPGSKAGGPPPPPPPPPPGGKTGSAPPPPPPPGGPRPPGPPPPPGGAPPLPPGPRPPGGPDPQFGASTGKQKHKPNVQLKQVCWTVIKPEQIKNTIWEQIDDTKLKLDFSIIENLFAVKATQSNASNQSGANQPQKPAKISMLGPERVKNLEIVLGKLKMSNQLIVDSLYQFDETILKPNVVESLITAMPNETEIGLWQDQDQSNLALPDIFCINISSVKGYDYRLLSLKFKYNYKELAEDLEQKIVGFKKVIERTRNDKNTKVVMEYALAAGNYMNGQSARGGAYGFKFDMMEKLADVKSTDNKSSLLMFIIQKAEEDLKQELVMVDENLDEIELAAKTPLSQLGADLNELKKNSKTVDKAIKSKVSPPIQDLVEEKLQDFYQQVITQLAEFETQLKQLESQYEDLSNFYAETKVPFDKFFEKFYKYKTVLRQAKQNINKIKLAEEKELEKKKKLEQQQQNQQQQKQQQQQSQQQQQQQQQQQQQQDQSQIKKKQEDNSLSGLKKLDKTQILNEVAKRRESKKTSILQLANVLVKQQIQTTLAQTQEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RZ89</accession>
    <name>A0A8S1RZ89_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0020332</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RZ89"/>
    <dbReference type="OrthoDB" id="307286at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="75"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="146"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="228"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="307"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8133296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="788" mass="93415" checksum="AEACCBD7A3738A46" modified="2022-10-12" version="1">MISNSQTKYHVKFEDGTIKEIECEPTQIKKIISKSYHLPYENIILINTHNNRLYSASNSFIQFQNKSIDLNVLDINQILKENINSKKKIIQLQNNEDDLQNEIVQLKETSREIITQMQKNKDDNQYIIQQKDQNFQQFQRMEQQHKEKLTAQLQLYEQKIAQINRNKEQLQQENENLQRKLQEQIQIAQKQERNFINEKFILQQKGDNQLNFNHQIYESQILALQVDITNMKQQINKQNNLIQQLKDEIEDIKAFYKQQIQDLETEQKNLENHYKQKIYKLEKGINEINSSINFSDNNLNFQIKNICNEAIREIDQFQQEMDNEEKNRNFQIQMKLKEGNNELQLKIQKLQLQIKNISSQLKIAETDLDYQQKKYQEEIDILNVQLNKAKKDLDDKQDEVNQYVLTNNQLQSEKQELDQYNQEYEHRIQQLEQAKTKLENNINELEEKYRFKHNQHLLSEQTIITKNGEINMLESVIEKQKQQINKMNQKIGRLTEILKQISNKNLGKGLEKVISPVGQLLNSRYGVQKFKDNPNCQSDETDYLVYRIKPSNNFIQSIRKGQFINLQQLLDVDPIGEIKSSRSIQPNEEGDLMKVYIIKVEDTQIKELRNRYLIRKDLQKGQNYDNKIDAAALSLNNILTQVFMDQFVKDLSELSIRVLQFSIAPQYVIQQKGKELYYYCEEIIENNIKQINGGEQDLGSSQDEQFLNSFSKYSHKLSQSELVISYINTVQEYMYDMIVTTEFGCFSEIDQGKDEYIRAIDAMESAQAINFGEFYNQKLELKALEFIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S202</accession>
    <name>A0A8S1S202_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0020411</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S202"/>
    <dbReference type="OrthoDB" id="291136at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8133450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="348" mass="41063" checksum="31D3145CB9192367" modified="2022-10-12" version="1">MSLIYKNHLVFPTFKHQETITTHKSSSLRPIKRFQSVTPIRQGLSSKIRPLNITALPGNETTINGDSTITPRQKHLPILTPRIQRSDEQNLHDYLDHLNSIRKQKQPTKQAVMKKIRTETEIMKLESQTLIQRFKKVKEQQEQKKKYSLLQTIKNPYAQQYINFSKQIFNLIHNLHQLKEYLNNSRKMFQVVKNLIGMKKNLKKILLKQSADELIDFQDIAKMCCIREIQYVNGEQRMIDKFDLSLMDDLTYMSEAIENLLLQNKLNAIKRMSDNILIEEYEISKLREQLGMREEDQKLSVSTLAMQRFKPHLELDNIEQRLGQLSQIPKQYRQTSQVLCEIVDKLNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S286</accession>
    <name>A0A8S1S286_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0030255</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S286"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8134338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="177" mass="21740" checksum="1D6956DB86F1E7BD" modified="2022-10-12" version="1">MRSIDINDEELKNILQEYLTDEEQKKYVFERSEDYFDQFDIFKHLVKEQIYLAILPRGQFVLYQKNEKIDKINMDDSINIIKEHLQQFNELDQTQKEFHLHRILYNMKEQIDGELIQIQQKTGKKEFRNMEEKLDYENEMKQYTGKSLFSIPTFDKTLVHYNWDNMQQIPQKLLFQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S2Q4</accession>
    <name>A0A8S1S2Q4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0030331</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S2Q4"/>
    <dbReference type="OrthoDB" id="285248at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8134486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="315" mass="36064" checksum="A8FA46F3971BC275" modified="2022-10-12" version="1">MRRPNEVEYNKTALMANWYEDRLAPQQLQRDPNATKSLRGVEEGISIPGDNGCLLPLPRVNRNPPYITKEIIVPDDGYREFRTEFQTKFDAKNIQNMQLGDCRSLIKTNGDKVNFPECNTTVAQTSGAKSIKQLDQTNMHQILVDRAVHQNYTDFGSTFKNHPEQHNKFYAITTYQQSFQQPENNVAEILTDKKQKPLAAGCRERDPFDQGLKMTSVITGEKYRDSIDPKENTQVQRQWIHSGDAGLTTAQNNLRQSSMDNYKKNASQVHPYDIATSLPLADGVYTLHSKYTDPGQFRHIRSDVTRIMNKPITRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S602</accession>
    <name>A0A8S1S602_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tubby C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0040066</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S602"/>
    <dbReference type="OrthoDB" id="8775810at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000007">
        <property type="entry name" value="Tubby_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517:SF7">
        <property type="entry name" value="PROTEIN KING TUBBY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517">
        <property type="entry name" value="TUBBY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01167">
        <property type="entry name" value="Tub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubby C-terminal" evidence="1">
        <location>
            <begin position="326"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8135125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="418" mass="48756" checksum="996315FF8E2D4A3D" modified="2022-10-12" version="1">MFLKDFIIDDINKQLQVNQVYGQSQVDDNQIQKFQGVVIIKNGAYTKNLGTNEAQYDIQEVDFNSILFDNENENKNQLRFQLQTIDSTNSLNQPEIVDFEQCRASIEEGCQERDQQQQLKNQEALEQEISNQNTISETEQEQVQNEEFALKTEDQNFEVLNFTEIQIPEQNKVIASKPYSDIIRSLDQSDYMGEFLITPLKQSGMFQCVIIRESFGLNSFKPKYKLYWQEKKQFLISARKQLNKNKYKLSQDPDFSSKFDAALIGKVEQSKQSKADYFLFDNGIKKNNQKNSIINTKPRIQLGGVFFEINQSSSKQPRKMMVLLKQNGNENQIITFENRQPILKKQFDQTIYTLDFFGRVKQASVKNTQLIMKNDIKDLIYFQLGKVNDQQFNLDFMSPFSPLLAFQVALTIFDFQSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S900</accession>
    <name>A0A8S1S900_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0040269</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S900"/>
    <dbReference type="OrthoDB" id="293758at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016589">
        <property type="term" value="C:NURF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038028">
        <property type="entry name" value="BPTF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45975">
        <property type="entry name" value="NUCLEOSOME-REMODELING FACTOR SUBUNIT BPTF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45975:SF2">
        <property type="entry name" value="NUCLEOSOME-REMODELING FACTOR SUBUNIT BPTF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="41"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8135529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="606" mass="71094" checksum="2EF5188EE69126AA" modified="2022-10-12" version="1">MRPISSALKLKTNDSQEKRLRSAVQFKLVDSQKLKNVLIKQSQTSQQSLSQQSRLQSGTTYNTTQRTGYSTHRMNEAEYDSGSNSAFRGQIKMRSTFHSSRNNLIRRVDSQFSLDNKQESSPQETNRTAIPVPKNRLFQQASRSQQKQLIVTQENIDSNEVKRRMKFDPQVKQQVIYFKILNISNIGLKNYLLYLYKQQKHLIDLVKSKAENQLIDFSQQILTFSIGTLSPIALIETLSFAASMLELTSQLDWAIFYHNQTRIMANYAKQTMDKYKMKSLIGLGNCSIKMQQYEIGIKFFKKCLQYSWLNNDLDYENEVYSKLGVCYYYLGDIEKTKYYHERAITYDYEVESSPLRQMSSDTLKVFLNKNFNRNYAETINTPLLNRLNLPLPQDILSISQQEIQNESLTMINPRIFNESKVSSTRRSSIFETNPHIKLFSTMQIDGLRIQQEILSTQEFEVEVYTPKHSFKSESKCFDFIRNKKIHPQDIFHDIKYKTNPFILSDLGVIKSQNDENPIENPQIYKLPLDQQIDIRLKKKIYVDIQAKLKKLMDYKYHTKTQMKNKILITHKNVENEKRHYAADQIKILENVSKNFENLIENLINLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SE73</accession>
    <name>A0A8S1SE73_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0080080</name>
        <name evidence="5" type="ORF">PPENT_87.1.T0080081</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SE73"/>
    <dbReference type="OrthoDB" id="314937at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="3">
        <location>
            <begin position="16"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="120"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8139401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8139403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="198" mass="22912" checksum="5F94F172E61395B2" modified="2022-10-12" version="1">MLLQFLLVLTEVQSISLKFKIPPGQQQCIKDNVSQNTLIYGQYESSTLLYTFTLSIVNQQEMENIIAEYSSQVVQQFHHVMQESGEISMCFEVDDYSEIATINLFYESGAEIYDQDQLAKKQHVLNINETLQQMEQLQQEISREQLLIVDREHKRKLSFVDIQSKIIGFAGITFGLLIIVAACQVIYVRRYVVYKKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SGB4</accession>
    <name>A0A8S1SGB4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CCT-beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0070388</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SGB4"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03336">
        <property type="entry name" value="TCP1_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000002">
        <property type="entry name" value="T-complex protein 1 subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000017">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012716">
        <property type="entry name" value="Chap_CCT_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02341">
        <property type="entry name" value="chap_CCT_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8138938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="536" mass="59393" checksum="171432BF2FA753E6" modified="2022-10-12" version="1">MDIVGNVLPQVLKDEATEDKGEMARLQSFVGAIAVADLVKTTLGPKGMDKILKPTGPGQEMTHITVTNDGATILKSMYVENPAAKILIEISKTQDEEVGDGTTTVAVLAGELLREGEKLIQKRIHPQHVIAGWRIARDVALKRLRDISSENDIESQEFHNDLVKIARTTLSSKLITQDRDYFADLCVKAVLRLKGSSNLDYIQIIKLPGGTIRDSYLDDGFILKKQITIGCKRRIENAKILVANTAMDYDKIKIYGTKVKVNSMDKVAEIEAAEKEKMKHKVDKILKYQPTVFVNRQLIYNYPEQLLADSGITVIEHADFEGMERVAAATGAEILSTFDAPERRDSVLGHCDLIEEIMIGEEKMIKFTGCKKNEACTIVLRGSSIHILDEVDRSIHDVLCVLTTTVKNRRVVWGGGNSEMQMAAACEEEAKKVQGKQALAIEAYARALRQIPTIICDNGGYDSAELIQNFNVELQKGNLTFGLNMNDGTIGDMQELGVKECMRVKEQAVMAASEAAELIMRVDDIVKCAPRKRDRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJZ9</accession>
    <name>A0A8S1SJZ9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0080229</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJZ9"/>
    <dbReference type="OrthoDB" id="293758at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8139657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="318" mass="37607" checksum="3027F828A99EF669" modified="2022-10-12" version="1">MCSQNNHKHLNHHYQSRLQSVTTFNTTQHTGYSTHIMSEAEYDSVPSSAFRGQIQMRSNLHSSRINSPQETHRNARTESNNRLFQQTNSSQQKQLIVNQEKIDSNEVKRRMKFDPQVNPQLIYFKISNISNIGFENYLLQLYKQLILSQILTFDLAIFYHNQTIIMVNYAKQIMDNCRMKSLGYYSLKCNNMRLELNYQKNVYNIHCQITIRIMKMKFTQNLMFVIIILETLKKYTIYLSCLNIIISDILKVFLNMNLNRNYAETINIPLLNQLNLPSQRYIVQEIKNKSLIMINTRTFNKSKTSLQEEGLFLNKTFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SKP5</accession>
    <name>A0A8S1SKP5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH CENPB-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0070406</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="307903at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006600">
        <property type="entry name" value="HTH_CenpB_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03221">
        <property type="entry name" value="HTH_Tnp_Tc5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51253">
        <property type="entry name" value="HTH_CENPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH CENPB-type" evidence="2">
        <location>
            <begin position="73"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8138974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="974" mass="113588" checksum="9A70AF61446928D6" modified="2022-10-12" version="1">MNYNNSSNSNTKRQNKKNKALKKYNILGQDDKQQILESLQKDSSLEMYQILSEKYNTSIKNLRRWYQDGIARKPGCGRKKLNTKAEEELRQWIIKESVELRRRVRRSQLKQKAIELFNIPNFKASKAWQDEFIRNFDIKYQVNEILYNKGILNQLQKVKFEEQRRKRQQQSVDQKSEKQEQLQQIFSQQATEIPQSKLEVLNIQPKVSDFKLEDWSFEKQEEMKDYLSEVFSPKLEFFNDEKSNQYFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIIFFLRILQFNIKNSRHLILLLSYKYLNFKQTASNFCQNPNIKNPDQFPQVMEQFDRDVCQNFGQAYPQVPKYQQQQLNIPQQNSLVDHFSRQTLFADQGQLFQYSQTLENAKIQQENSKPIQQKHLLMDHMLGNQFVPVPNNYLDFNVNSNQLGQIIKQPTYFRDPHDVNEFQKKQIQYLQAINDPSPPDQVKLFKQNIQQLGGIEDTIGNMQQTLNQYEIKHLNNKNIKYYPLSTPPLTQEQIQYQNSISSNTHPFLYNPADDKERFVYGPLKPAQNILEQQQNHQTQFYQTMRQQYNQDLNQIQERTQPLQQQYQDQGYQQQHPQSFYFNSIEQSKRLRMKKYQQLQQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SLA4</accession>
    <name>A0A8S1SLA4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0110039</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SLA4"/>
    <dbReference type="OrthoDB" id="286446at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0349">Heme</keyword>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8142241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="45" mass="5170" checksum="9F7FEED4B255DD31" modified="2022-10-12" version="1">MTILYTDKLYLPFSAGPRNCIGQHMALMEAKIILSHLKIVYYIAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SQR6</accession>
    <name>A0A8S1SQR6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0120436</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SQR6"/>
    <dbReference type="OrthoDB" id="286816at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8143851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="182" mass="21277" checksum="EA5588F5007A6B38" modified="2022-10-12" version="1">MNTSSKPFIIRHIQENYLVSSDSTSFNAIIEKRIQFVQGANTFIAVMLSYDSIQHGFQFFQYYDKCLSYAYMIGALLIGNIFWILCSIFGFYSIDAKNSQKIHRILVYGYMAYQMNQFNFYPESEKVISDIVCESLNQTGLIIIQITIESVVLLIMIYFSHSIRHYIRLWEFNENSKNWGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SQS9</accession>
    <name>A0A8S1SQS9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0120052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SQS9"/>
    <dbReference type="OrthoDB" id="307110at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="27"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="259"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="427"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8143125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="657" mass="77657" checksum="D901C1547ACC2E0C" modified="2022-10-12" version="1">MSVQKYKKSGSCKSDSAYSFSQDVNLLQDYEYLLQKSAEELTKVKEQNQFLEQQLASVQPFHHNQQQYSTFQFNKSDLLIEQLKQENADLKLKLETKTKAHKKLKTKLKQHEQIMQENENLKRDIQDLLKEKINNKENRQNLINQSTKTPQQQQQQNVNQAPCNNFKLDLSLASNFNFKGLEEADKFLQETKIQSSRVSKMQTIPTPKRYQEIQTIEINFSDFDLQNNLSIQQEFVQFIEQVIFKANNQLNQRIQTSQIDNFIKSNQDLETQIIQLSEEVWILREKNKIQQEIIKQLKHNLECCDQKDIEELIKLNQQANDFIEIQQQEIKSLSELVIQTEQELLQKSQIILDNIQIIEKAIKKATIQDNIQDENKENQSCISNQKLQKKQTKSSNYSSLQSTQTLNKQSPQLSIDLQEYNEMKTNFQKVFQEVEQFQQLLNEFQEQEQNNNISNQDLFEILELFQEEFSYRENTSIKDQIKYYITQTLNQNELLKNKYLDLIYKVILNQIKTDTRRTELLVKNDQKLQDALKWLLQNTKRSQSVQTIIYNCDILTDMIVKKLNNSTRMSELHEIITMQMKVIYTLLQSLQIGKNNLQSSRTILVKNEKQETLMNEAIQDLKDLNKKISNLPDTNDDLESSDVKENIRQICNKFIMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SRC8</accession>
    <name>A0A8S1SRC8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0090404</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8141032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SRC8"/>
    <dbReference type="OrthoDB" id="311826at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8141032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="212" mass="25467" checksum="90E661D655DDC44B" modified="2022-10-12" version="1">MIQQLLDEMTVASEKQQKEMNKINYKHQRRTRDVINAEKQEKLENKEIDVGLPRIKDKEEVYQELKRRFRMAWKSPGFKNDEFTKIPTLKGKILNEWEILRINLAERSYKAPQGFKGFKIPSQYQEFQKPKTPPGIKKQLDEFKLDFEDFVQRPSKYGKVHEDEILEKVRINNKEPFRLTNSVQHNEFGFDPHLTWEAEMKRKQSNSKKLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SUL6</accession>
    <name>A0A8S1SUL6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0140179</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SUL6"/>
    <dbReference type="OrthoDB" id="162894at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05579">
        <property type="entry name" value="STKc_MAST_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000604">
        <property type="entry name" value="AGC protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000633">
        <property type="entry name" value="Protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000340">
        <property type="entry name" value="Serine threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE GREATWALL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="655"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="383"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="202"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="997"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="383"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="409"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8145311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1073" mass="125167" checksum="89CB14DC8B85C04E" modified="2022-10-12" version="1">MQTDEPFVTNFYIEQQIKILKPHPIAIKLSVDILQNYYQLQARESIRTLQSQLLKIYKFFQQDQFEGQQEINDKINNLLDKIKKIDDNSIKKIPFRRSVNRKSLAKYFSCFSNLNRSFKMLSFKLRISFDAIIIIWINKQSIPNQLKWSCQCIKNIQNKKSQLNQDQERVAKNQLKMFICRICEQQMQAIDMNQHCIQCEKYAESKKRLIELNLELANLCDQAYQEKRNVQIKLAIKKMKDKNKQKQQTNDQIYFRRTQTYDDQDDEDVSEYQQEQNQIANIMTQIINFAEKTLNNEVEIEDTKLTLILLNDLVSSTEYIENQEALNVINSTHKCLLERLEYHKKQEVYKNNVKNQSQQDQSEDNINKIRRAFTKSNSISFRLPKFQNTPSSSLQRISTDPIQEEEDSPTQLQMKSSQKIISQRRGFRMNSSIFKVSDGSESPKSLKNIQTSINQSKSNSLNNNESNQSINSNFTDIKVDQFQKYSNNEQQNEQDQQSIAEVKLSSFKAQLQPLTVEKRQPSLQEISPVMEPLNLSIKEGENSSNISSFDSGQSNSAINNDLNLLALQKRTCKQKQRKKQNFQSQDIEKMQQCHYKTQHRKSRFYENQICKSPIVLHDYGMDEIQIDKGYHSDSNLIKTTSYQQNQVSQVGIKDFEFIKPLGKGAYGWVFLVKKKGSGDLYALKIIDCAQRNLEAFLEQLKAERNIFEILNSNFVVKAYFSFVHEQYLCFVQEYMVGGDLASILKTYTALDEFYVRHYMAEIVLALDYLRQQNIVHRDLKPENILLDCQGHAKLADFGLSEQGVNSRLKLKDSMNSQNTIEIPTCVEKMIDEQGYQTVYKQLKKVESILVDKPGSKTKKIVGTPDYIAPEIILGTSASNFSCDYWSLGIIMYELLCGIAPFNDDTVDKIFDNIVNMRIEWPQIGDAEDCISEQAQDLMIQLLEPDFKNRLGHKSIEEIKNHQFFKGISWNTLLSKPGLIVPELNFELKDTEKMLQFLKKLEKTNKDNENKKLTQQLKAQLQSLERIDLLKQRSIQESEKYLQQVKNEETKLNKQIDSLTQFFAKIYQTYSQMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SXM8</accession>
    <name>A0A8S1SXM8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">STOP protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0150331</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM154 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SXM8"/>
    <dbReference type="OrthoDB" id="294947at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033336">
        <property type="entry name" value="SAXO1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31516">
        <property type="entry name" value="STABILIZER OF AXONEMAL MICROTUBULES 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31516:SF17">
        <property type="entry name" value="STABILIZER OF AXONEMAL MICROTUBULES 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="257"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="261"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8146411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="308" mass="34900" checksum="E1C6A3C42A6D785E" modified="2022-10-12" version="1">MAQITQKQNNSNQINSNLQFSLNQKQAVNQSQNIDSDLISSKKKQVYDRLMSSQTNRDNQTEVSQYFDHLGGKCLCALCNCGKHKCNSKNCIHKPQLHGNYTIYQKEYQKKTPETGSRYNQNIFTQPKAEGDLENVTTYKHDFPGYNSKAELHKNSQKPTVSGVPFSGLSTYNNMYLNWGMGDTPQLLPQNNPTVIKEMPFMGRSIYKDSYQGVQALPTQSCKNMNKALKSPLSPPDLKFSAESIAKSSYKPFRTDRVPTAKSQQNATLNPSFNGQYNSEYRKEFDPKGLNQCPAKEVLEEVARSTKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SXT8</accession>
    <name>A0A8S1SXT8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0150036</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8145835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SXT8"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000085">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="203"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8145835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="489" mass="55116" checksum="D76E173B04FCA84E" modified="2022-10-12" version="1">MKINSASQQKLAMTRPQTTPQKVGQSPIKKNKYLVQSGVSSLAKTDNKVSKSTALSHDLISLMKNGGNGHHNSQQLDRKQNSRSKCILDINDANLKKNRPNSRQQIQNLVKSKKPDSNLKYHEELIQKQFQKLLDLQKQHQQTHKSPPQIHSRAMSNGDLKPTSDIANKLIEYIKKQSAKKIKGYRSISNPDIIVTDRRLISKYAVKTQAGMLHTKQEKVNQDSYAIHEGIGNIDNSYLIQVSDGHGVNGHEVAQFVQERLPIIIDQLLKSHKIGKNDQDMMIQVILRQAFERTTKELYTSGIDITFSGATTVCLLIIEHMGWCANIGDSRAILGRQKDGLHVVELSHDQKPDLPKEEKRIIQNGGRVQAYSDEEGNPIGPARVWLLDENIPGLAMSRSFGDYVAAQVGVISIPEIIKHTFQNDKFLIMASDGIWEFLDNKWIVEVVYGYYLKNDAEGAVERLVKEATEAWQKEDEVIDDITCIVAFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T2N1</accession>
    <name>A0A8S1T2N1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0140005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8144982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T2N1"/>
    <dbReference type="OrthoDB" id="302887at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8144982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="328" mass="39202" checksum="722AB2B5BEC8C9E9" modified="2022-10-12" version="1">MSLILQNQQKKNEEKKELIAKTIRKQKRKNQYTVEFPFGSKIDLSILKSISKISQILPPSIPSNIVFRLNSNIQIPQSFNQDYQLGCLSIDPHSFNYKVIRYDKCQNFHIFHFEPIERQYTPASVIKDLFDKQNTRLGQVFDNLHSTKLNTEKFKVVSEQVIDEFEKKISSAYSLYISKYKDGTISTVARVMNDQYLKMIGINETMIYDYITSTGCLPWPYQQTQDNQWDYNLLVLFTCNEKQVPIQGQAVNYNGNLFPVFLNQYNYYLYNEQEQSYTQFQYYIYEYNEKWSNDKQIRQNYLNYYNKRLDTLEQMDPNIIKRCKYKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T5T6</accession>
    <name>A0A8S1T5T6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Palmitoyltransferase</fullName>
            <ecNumber evidence="10">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0160101</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="10"/>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8146886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T5T6"/>
    <dbReference type="OrthoDB" id="302728at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF43">
        <property type="entry name" value="PALMITOYLTRANSFERASE APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="38"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="304"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="11">
        <location>
            <begin position="158"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8146886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="381" mass="44543" checksum="31328885E478E9B6" modified="2022-10-12" version="1">MNNIQQFFEDIDTMESKKSFEAFTRCKGTIIFGAKKDFFNIIGTLTLIIALIVLYLIFIFPAALDYQYDALAIIIVICCILPIITLLNVTMTEPGVLLRGDLPDPKLQQQQTQPEQISQQIYNSSEQQIKRNEPHQIVQITEQQNQSEQNPELPNIYTSRYCSTCKIMRPSKASHCKHCNHCVDGFDHHCFWVGTCIGIRNWRAFLLFLQSSLIVVILTLCQCFINLSQQYLDIYELWILMFKYASIPLIVIYGLYFLCGCWNHQTYLNVIILIIMFILPIIYSAILMNIEYTYKDYRYYENPFITILVTVIVLVCFLFLIPVNGLNCYYISIGKTAKQTKSEDQFYIRKKAFVKQTYTFSGVFRNLLYFYTYPIPQSRNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T6M2</accession>
    <name>A0A8S1T6M2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0180306</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T6M2"/>
    <dbReference type="OrthoDB" id="301809at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="472"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="561"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="472"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="578"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8148915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="596" mass="70082" checksum="35BE5C92D79685A6" modified="2022-10-12" version="1">MSFQSISSSDLSDSDEMTFIKTDQERILKNSNILKNDNNESNSCQRHPNKKAKFYVQQNSSQLFCSKCALNLALQGLQIEETQEVQLEIQRKDKINNFQKDLHQALQQCDQKLVNLNDIKDKLIRQWEEQNMNCEEFFNVVQKTTNQLRQTYYQKFQNDYTTSQNQVQEQVDNIKLLIQQLKQFSIDIFTNHDNIVKKMEMKPFDEIMQKYQKKVDEIKCQSFHSQFQISLKQLNIDQNQILTFMNKMCYNLLIKNDSMEYNQKTMTSQQNFECNLSNNQNYIDIIENEEIHSPISIMNQIQLQSPIRQQFNKFDSINSNPVRSNANTPESWKQKPSLRRDNITPNDKISYQNSSRVLEQVSSKKALQKQLIDKYSYQCNEKQKTTTDYERISEKSCSILNNNLSCFQIQKFLKEAERGSLEDRELTPKQKVITASKSIMYGQNYNNQIKNHSNQKSQQNLIQNYLHKQQNGSVPNLKSVSHQQKQNDTVQLHRQQEGHLRSKSKQIKPQSIEQDSKKQFIINAGVNSVSLPQNEDTLKDKILKELCSHPSESVYGQVLKNHQQKQKNYKPTSKENFEWTSQKNRTIGQNTNKKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TAM9</accession>
    <name>A0A8S1TAM9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Plasminogen receptor (KT)</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0190140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TAM9"/>
    <dbReference type="OrthoDB" id="308865at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019319">
        <property type="entry name" value="Plg-R(KT)"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13411">
        <property type="entry name" value="PLASMINOGEN RECEPTOR (KT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13411:SF6">
        <property type="entry name" value="PLASMINOGEN RECEPTOR (KT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10166">
        <property type="entry name" value="DUF2368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8149500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="131" mass="15618" checksum="6931B0F99D112D43" modified="2022-10-12" version="1">MKENQKEMQKEMQKKQLEMMLKQRQTQLAMQFASGKEFFNWFASFYALIFPLCILGALKKKSPFPIIPLIPLGFVCGYQYDMYYGDKMKRIRLEAERLIDSEPQLFYFPKNAHIVSQEEYEEILGINKNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TB19</accession>
    <name>A0A8S1TB19_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0180256</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the proteasome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase T1B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TB19"/>
    <dbReference type="OrthoDB" id="429533at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03763">
        <property type="entry name" value="proteasome_beta_type_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF4">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="4 6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0888">Threonine protease</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8148817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="275" mass="30232" checksum="14E67DABF8D27EFE" modified="2022-10-12" version="1">MQYLDNYLQSKGGYDYTNVKRNELMKAQGFKEMPFTKTGTTIVGVLFDGGVVMAADTRATAGSIVADKNCEKLHVLAPNIWAAGAGTAADLHHQCAHFNAKLKLQRLNLNRQSRVNEVITKLTSKLFPYRGNIGVALIIGGIDCNGPQLASVSPHGNYVYHPFQSMGSGSLAALGILEAKFQDGLTKQQAIDLAIEAIEAGIFHDMGSGSNVDVVAITKDGADYKRNIRQYNAKSYQRQIPYDFPINNTPALRKYQFDIEKQELTEVGQEMEIIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TBM6</accession>
    <name>A0A8S1TBM6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0200367</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TBM6"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="269"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8150605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="551" mass="64659" checksum="619A580B414172C9" modified="2022-10-12" version="1">MHYSFQYQKLNELFLASGESSQKFKSTDQMYRKLGDDFEYMLMKLLGTDFDKFNLIEQLNLNNQSQMQDQNLVVTIPILFHPTLYGVSMDIIYRPKKICLKSYLTKTDSSPLYASDKNIINLISFYALKQNDKLINGSLSIDPLTGLFQYKTLVVISLYEQINLSENFLESLKLNIKQHKEVMQIQAPIILYQMNKITFKEFKRFANEESVENKEKNIDQIRKIFEKQLNKEMTQEEKENFLDCPKPNNLPLLTKIKIQNISDDPIKQWKEQSLITSGGYSEIRKIQLAYSIKDDQTYTRALAIKTDKSPNNYRKVNHELNILKNLSETREKGIGEAYIVTSYYDENLKDCYFMEFYNNGSLEQYANSKAIVLSLRTKLFILAGIINGIDFLHYKQIAHLDLKMGNILIQKQLIPKICDFGEAKFFQQLDQDKSVFSRSLPYAAPELYNKNEITPAFDIFSYGILMCNLIFEQFPFDYNPNDLYNLAERYRNNTYAIKRNLIKQTKYGPKKIMKIILQLIVHCLQSDPKLRPKPKWILAILWKMINFLDSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TD82</accession>
    <name>A0A8S1TD82_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0180292</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="542352at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000241">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057352">
        <property type="entry name" value="TPR_TmcB/C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25474">
        <property type="entry name" value="TPR_TmcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="266"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="328"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1217"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1343"/>
            <end position="1363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1553"/>
            <end position="1574"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="2">
        <location>
            <begin position="646"/>
            <end position="677"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8148889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1616" mass="190233" checksum="32374350612924C7" modified="2022-10-12" version="1">MQDQEKQGIDDALMMGNQKRGIDYYLEQLKLTIFQVLFVIRNSEDEEGGMFGFYFFNALDYFQMHQFPFYVTIYYLWKQDTFMEFITLVQNLFQISNYIPSLTYESLLMTVYILLFFILLIILDIIYVSYSFSRNRFYWLWPLLLLKKITSFVVTVGFLPITETLTSILQCYVDPDTGKYIIYGYNNIFVECWQDWHTFHAVLDLTFIIFFSIICSIVAYAFFEPAMNTNDRSARQDSNGEVAFITNKITCQLMFSLLRPNYPEQAWIIVITSFILSLWLFKQYNFNDPYYDFQVGLFYCICSTLYLWACFMLLVCKVLESTDFTGGFIAWLIGVPFIISIMLLTKKSKIETLVKSQTKFRSGEQIWSHIRYVLQLIQNQDKDRNSYMLLVGYIEKHKETCNYDDCPLKTKLKQKKKGSQNDINEIIRGLIQELDRMFVQGLKKFPNSTYLRIFYALFLIERRNNKQKALEQFELAQYTKPALYQEFIIYRYKKDIKSKGMNSQQGEDDDIVNAIAFKNYLSLCEEKMKFSANLHKEFWIELKEDQPDLGRLMNIGARISRVTNEARENYENMQKINSNMPYTMQVFGNFMIHVMNDSKGGINLLNKAKQLKEHLRKNQQVHKDIFNFETDQIPFIYVSAKKSDTGNILQMNSLFTAYFGYTKEEIVGKKINILMPQKYAENHDKYMSNFFDNFVLNLRQEVGIESEYLDVDQNRLFKHKNGYLFPLTYQVTLHLDSLVYIATFKSEATLKTQIYFVIDKFSQILEMSSSAITFFELELKNLGDKIKLNDFIPDVLTKNAKDVFSKIPKKDGFYYFNCQVKEIILPFHNPNDSDEEQKKSERTGCYVIRLDKIEKNEANLKYMIKKGGQHTGSISNPDSELILKAPSQSKIQNLANMSQDSQVPLRNQDVDLNLFSYMQELYENPQVDEIRDLFKQCNEETESVPSIDLAGSIITKRYINGQIVSINEEQEQLFLQILEEEEEDNSLFRNQGNNFEDEDDYKLFTYNNSANLIQIALRTHHRHPEITTFKIYSSLWIVFILAMTSLQQYFCSEQFNNYRIDVDVLQLINNQLIEINRLTSRVLDLAVYSANLYTYNTTEIANDISISATSVIDFNSAMNSIDYINFQSKFEDELLFRFYTDNGYYDDSLRLETALVVFCSLALNSSVTNYQSITFNNIYYQGVVHNYFDVLYQQICDISSSTYDNIKDIMDNPQVDLLILLSASIFVSVMAMIKFIFLMFAIKTQRENILFLFLDIPTYHLDVLYKKCDRFLKNYVSIEELQNKQEQQGFESSEDEKELQEEKIEVLKAPDEDESDILLRQTQNRKKIIKKYRQNRIKGNQGIIIQFFVISVFTLVFTVYNILGSQSQQQQINFLLPQYYQSLYTINNYGYYINIQKLMMLDYTIQIKGIPVSEYAINNLTIFTGNAEKMSSFNFDILSELQTFRDKYINIYYGNLCEQIMKTAEMDQCQQIINSNLQLGMYNTESYFLEYFRINIQNFYQNITQNSLLLNATQFFEIDRSLYNYVYDAITNIITYERDLISSGMDNIQNIQLILLIIFIVLLVSIFLFVWIPFLNGLNTQINQTIEMLNMIPLEVVKENKSIRRFVKSLIKSMNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TDR7</accession>
    <name>A0A8S1TDR7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0220329</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8152201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TDR7"/>
    <dbReference type="OrthoDB" id="308959at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8152201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="139" mass="15945" checksum="6E19493873162732" modified="2022-10-12" version="1">MIRLTHNKSVACFSGALWGPIHERPIVDRVMSTSQWPVPYYQRIFKAYPVRQNKQTWAMNLAGAEIHDINWYCAKQALSRTLKGRQAVEYVENNIPTQSYIVIQKDVSRMAKAYVSDLSLFLSVANKESKVILDSVELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TQ22</accession>
    <name>A0A8S1TQ22_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WD-40 repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0240319</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1141"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1165"/>
            <end position="1185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1219"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1254"/>
            <end position="1281"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="218"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="260"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="302"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="344"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="386"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="428"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="470"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="512"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="554"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="596"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="638"/>
            <end position="679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8153627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1395" mass="156988" checksum="621819C1B1E64B0B" modified="2022-10-12" version="1">MYCNFEQKEALIEIFAQVKDIDEQIFSVIIEIFCQEKVQDSIGYISDNLNQKHFEQYILEGEYLKQVDNEQNLYVVRNSIKQIINILKKIKDHDFNKNDFSNEGTQECRQVLLNGMRNQRLIIEFLKFLVYLTSIDSSLIQCGSNSLHLLVEMKIDLTQKNLENIVIQNTSLVGANLVRCNLSGSQFNNVDISGINLNEAQLFNCSWKNIRIHELNQINGHSDYVRTVCISPDGTTIASGSYDKSIRLWDFKTGQQKGKLDGHRKWVSSVCFSPNGSILASGSGDNSICQWDIKTRKKKSKFEGNRGKVSSICFSPDGTTIASGNSDKSINLWNVNTGQQKVKLIGHIDALCSVCFSNDGTTLASGGDDKYICLWDVKTGQLIAKLYGHSDYIRSVCFSPDGTTIASGSDDNSINLWEVKTRQQKVILHGHDDSVLSLCFFPDCTTLASGSSDKSIRIWDVKTGQEKAKLYGHSDYVYSVYLSPDGKTLASSSRDCSIRLWDVKTGQQKPKLDGHDDSVLSVCFSPKGTILVSGSYDKTICLWDVKTGQQKAKLNGHTGSVLQVCFAPSGTTLASCSYDKSIRIWDVKTEQQIAKFDGHSNYVFSVCFSPDGTILASGSADKTILLWDVKTGQQKAKLVGHFQWVSSLCFSPDGSTLASGSGDNSIHIWDVSTGQQNAKLDGLSNCINSVCFSPDGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVLHQHLVVMISLFVYGMLKQNNKQLNLMVIVIMSSQYASLLMVLYQHLVVQIKLYFYGMLKQGNKKPNQLVIFNGSHHYASLLMVVHQHLVVEITLFIYGMLAQDNKMQNQMVLAIVLTQYVSLLMVLLQYLVAMITLYVYGMLRQVRKLKPLIKISKIYSQNLTLQYFYTILYLKSQLIQLFSQCHNNQYFKLKVHQFSKANLQIIKELIYNHYLNQKEVVFWKAQQQQSKGENWFSRTSKIKYYNYFQNFYYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TS74</accession>
    <name>A0A8S1TS74_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0250030</name>
        <name evidence="2" type="ORF">PPENT_87.1.T0250031</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8153786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8153788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TS74"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8153786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8153788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="111" mass="13554" checksum="B8AA1675DCE4496A" modified="2022-10-12" version="1">MIFKNLSYYITDQQEPKLEELRDLKLQDRGKQFKIVGEIDSCIAFITKFLVKQYKMFKDFKYQISLHLIYYSQCILINHQNSFVFILINMINLKITKDYYLKYFQIQVEKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TT13</accession>
    <name>A0A8S1TT13_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0270087</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TT13"/>
    <dbReference type="OrthoDB" id="321763at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="28"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="211"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8155430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="535" mass="64066" checksum="59BEB050401112CE" modified="2022-10-12" version="1">MQQGEEIDMKELEELITKKCKAREEEIQKQVTQDMTKYKEECEKILQNIQKEHEKIQKQYGQDQENFNKTLKDKINQVKSLVNTEKNKAKEELAKTQKNIIQLTQEQFDALSKSKDGQIQELQKQLKELEALKQQELEALKQQQLEQHNQEITKLKKDLVDLQSSTEQQLSKERINSQQELKNRITQMQNESTQRFKDQKQQHDVEMEQFRRSFERNIEKLMKEKSALQEQLTNAEIEKIQMKQSTQSLSECKDCIKDLQNANVDKETLEKLQNTVKNYEQAVSDMESEQKIFLTTKSQLSETQQSNQQLESKLEQKEYGLNKLKEKSEICQKRLKECQEQIQQLQIQLKEKSELEDKAKEMSLKYQQQIQKMKENFEKQEKELQNYKASLNEITQKFEQEQQKNLQLKEDFEKEKMRLQEENRRSQENIQKKYEEDMKKQKQKIEQDILSQKEKIETIELQKSNIKNSTLQQQKDHVENKMQKIRDYLEHYRNAMARGLQFKECLLSEINGFEKQASLYPLDQSLMFESGITTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TT29</accession>
    <name>A0A8S1TT29_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Arginyl-tRNA--protein transferase 1</fullName>
            <shortName evidence="5">Arginyltransferase 1</shortName>
            <shortName evidence="5">R-transferase 1</shortName>
            <ecNumber evidence="5">2.3.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Arginine-tRNA--protein transferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0250215</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the post-translational conjugation of arginine to the N-terminal aspartate or glutamate of a protein. This arginylation is required for degradation of the protein via the ubiquitin pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>an N-terminal L-alpha-aminoacyl-[protein] + L-arginyl-tRNA(Arg) = an N-terminal L-arginyl-L-aminoacyl-[protein] + tRNA(Arg) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="Rhea" id="RHEA-COMP:10636"/>
            <dbReference type="Rhea" id="RHEA-COMP:10638"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:78597"/>
            <dbReference type="ChEBI" id="CHEBI:83562"/>
            <dbReference type="EC" id="2.3.2.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the R-transferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.8" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8154129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TT29"/>
    <dbReference type="OrthoDB" id="293785at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004057">
        <property type="term" value="F:arginyl-tRNA--protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017137">
        <property type="entry name" value="Arg-tRNA-P_Trfase_1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030700">
        <property type="entry name" value="N-end_Aminoacyl_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007472">
        <property type="entry name" value="N-end_Aminoacyl_Trfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007471">
        <property type="entry name" value="N-end_Aminoacyl_Trfase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21367">
        <property type="entry name" value="ARGININE-TRNA-PROTEIN TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21367:SF1">
        <property type="entry name" value="ARGINYL-TRNA--PROTEIN TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04377">
        <property type="entry name" value="ATE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04376">
        <property type="entry name" value="ATE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037207">
        <property type="entry name" value="ATE1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="N-end aminoacyl transferase N-terminal" evidence="6">
        <location>
            <begin position="37"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="N-end rule aminoacyl transferase C-terminal" evidence="7">
        <location>
            <begin position="359"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR037207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8154129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="599" mass="70873" checksum="4D565768E4D8E008" modified="2022-10-12" version="1">MNSKLVLPISIQNIQLQTLKMSSIIQLNSVSIKNETNCGYCNGAKSNKLWVTSGFYCYKMKLDEYQQLMDRGWRRCGVYYYRPALAQSCCQAYALRVDTQNFKHRVTHEKVLKKIKRIQPIQNDLNDLHQKVQLKSEIKEDQFTQSILSQIQTDIDTKDLVILSKVGLHFTFKSIQYKEEEHIKQFKQIIINNLSQITIQELSQVFLITNDEHQNILLNFFRSSLQEFSIYEITVFRGQINKVENEWGFGCNFIPYFYSKLQTKFKSLYPQFQTLEENKEYIQLISKYLSNIDFIFKVSQWDNQILIKTPKTIKEKIFKSTVLYYLQKADVQNTQNLVKEKKNHQIEIKWVKAQFQEDSFEVYKRYQKAIHDKERVSKQSYLNFVCVDALDSIELGCWHMKYYLDNQLIAVGVVDLLPQSLSSVYFFYDPAYKKYSLGVYGVLQEIEYIKQHQKENPQLKYYYMGFYIMDSKKMAYKADYTPCELLCPQTYRWIPLSKQIKEKIKKITEYAQDVRLAEEKCGQLGYSINLDGSTIIDMDFSDVDIQNFILSHIKISQAGKQYSISQLKKNYQQYFMNLFENLLGKIGKQLMTEFLFAVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TU43</accession>
    <name>A0A8S1TU43_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0300093</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000030">
        <property type="protein sequence ID" value="CAD8157321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TU43"/>
    <dbReference type="OrthoDB" id="299641at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="214"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="251"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="321"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="333"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8157321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="345" mass="39080" checksum="6DA3A55EC6233D22" modified="2022-10-12" version="1">METDINIPKTKAVNVFFQFARMQFYLTVFSNLLPIIISQTNQVCSNEGIKLIALSYKGDIVLLILNLISLLFIWLKEPFARLEFFVMYAINICWSGIMTGQTYLDVTTQPNECEGVILSQKLILFFHLKTHIEVLLTLMSPILFAFRITNLPNSFYAIYLILTANSQQQLCSRDIPNIEFGLIFVALVSCLIGLPLNIGLFCTKKLTIPNLFRFLLMLTIIGQSGLIALIYQADVDLRSECSPTHLLFRSYLYIIPITILSALSLLFSMPVQDHDEIRQLLIQARLKPLKQQLPIQQDLTKPQVQQQQQQDEILNRSAIDGGKSQIGAKSQVGAKSQYGTISRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TUA4</accession>
    <name>A0A8S1TUA4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="16">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PPENT_87.1.T0260027</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Na(+)(in) + ATP + H2O = Na(+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:14634"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Membrane</location>
            <topology evidence="1 16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="16"/>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8154556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TUA4"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008554">
        <property type="term" value="F:P-type sodium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000317">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000190">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF150">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 15 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 14 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0915">Sodium</keyword>
    <keyword evidence="11" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7 16" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="296"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="836"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="869"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="934"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="964"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="995"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1033"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="17">
        <location>
            <begin position="2"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="18">
        <location>
            <begin position="805"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="13">
        <location>
            <position position="363"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="364"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="365"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="365"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="477"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="520"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="543"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="583"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="666"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="667"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="668"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="753"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="759"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="779"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="782"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="783"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="783"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAD8154556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1079" mass="125558" checksum="451A60108773E636" modified="2022-10-12" version="1">MLTFLPKNSLEQFRKASNLYFLLLGIFQILPPVTTTDGQPTVYLPLSFIIMVSMIKDFFEDFKRHRADDEENNRTVQKYSLRTGVFEYDKWQNVYVGDIIRIANKQRIPADIIILATSKGGECFVETKNLDGETNLKPKYAHPQLQTLYRQLNEKELPYNLFVTMDFERQNPLMYKFKGSFNIVNENKEQPKEPLNYENFLERGCSLQNTDWILAVTVYTGHDTKIMMNSIIGKMKYSTVEKLMSKQILWVFLFLLFECIFASTYYNVWYQRNVNELQSYLNIDQNAPENNSFYNFILRFAMWFLLLGNSVPISLLVTLETVKFFQAQMIQWDKNYLTFDRPAAVHSSNLSEELGVIEYIFSDKTGTLTQNIMKFKSIIIDDIVYGDIEEQPQQEIIDEKPFKEKQRIVSQNVDFTDDTLYSELVSEFMNKKSAKNFSNNDHIFLTLLCLSLCHTIQTELVENIESEIQYNASSPDELALVSFAAEMGFKYIGKEDNIMKVYIKHSKETLKFQIQQIIEFNSTRKRMSIVLKDQNGRLTLFCKGADNVILSMLQDFDENDSIQKNILFEIKKQLQEYATIGLRTLVLGYKELEQKEYEKFLSEYNRAQSILDDDQLRESLMNQLEEMIENNLQLLCATAIEDKLQDKVGEVIADLKSAGINVWVLTGDKIETAINIGFSCKLLTDQVKRFIIDGDVEGQVERQLAKVTNYINDHPDEPLSLIVSGVALVIIIHNFKEVFFKVALKANAVMACRVSPKQKQEIVNLVRRLTGKITLAIGDGANDVAMITQAHVGIGIRGLEGQQAAKASDYAIGEFKHLRRLLFYSGHESYRKNSNLILFNFYKNQLYIGAFFFFGFSNGFSGQNLYDQWLSQIFNVFFTSLPIILYALFDEKYPNSNYMQLVQDKANFLESRPDIYREYLTKPIFNLVSFWQQFLWGLLQAILLMVISFYAFEPISPHVHGQNSTYLEAGMTIMSAVVFIVNFKVLLLHNTNYPIIVYINIGSTTCFILFFLLFSQYPFFESFDLFQRLYECFNYYLAMILMLTVTNFFDLGRQRYNYFVEQYREQQQNNPPNQLEIEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TX81</accession>
    <name>A0A8S1TX81_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0290165</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TX81"/>
    <dbReference type="OrthoDB" id="308059at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8156780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="469" mass="56160" checksum="931FD5039F6D6DC3" modified="2022-10-12" version="1">MLNSKVQEIKQSSYNDYIFDSWANTNETSRVQTNQQTQLKIQSQRQKKFKYSNILYQNNDQIITTKSQVHQKYQDVQLNSFSNNLDELKQKEQYSFQKYQKVRSTNRNHSICRNQNEDLEIKYESIANCSTIFSQRIKNSQKKQLSYSQNCKLILPKIKKKQTQKILINKFLVICRVIGKIIILYFKTLSNTNPLKITNNLKLQKVLNLRKIFKYDRNISDSLSKSIREWVKPSLQKIFYHLQNTLIYTQDEKVLDLRKLKDQEQIWTLNFAKFLFQNLELITRKGNIPKEIIKAMSNALYQENNQYVQLFLAQRTKFYNYPFSILEIQLICSEYILFNAIVIELFDQANNLTYQSFNHNLNCKIQVLKLTSIINVFYINIFKDMPIINFDYKDEQLYSRKLYITNDQQQLLQLVDTKEINNSETLILGLKHQDYIQRILQQKEKQKQKLELIFNKFINNLYSQIDISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TXW1</accession>
    <name>A0A8S1TXW1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0290328</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8157080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TXW1"/>
    <dbReference type="OrthoDB" id="298910at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="247"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="157"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="283"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="414"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="253"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8157080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="567" mass="68224" checksum="775025BCC4D18579" modified="2022-10-12" version="1">MKSVIELLNKCNFPKRQIHNFGQIQDGILFAQLEDEFFKTDLKQYLWNLIITRLQRIRRNHNLYIKEFTQEIANSDCNDELAKFIKYFLETIFYVKKEDAIYYMKQCSDNQQEEIVQFMQSDLLESNSLQDLGQSEFQTTNTIISLEENLYSKDNIIIQQTKQIEQLKSDYQNMLQEMQTKIYEQQEIIDKLKAQADFFLEKVICDDFEEAWFKFQEFTQDQEKNKKQIEDLSNLVQNQQNEILNKRRKNQKLKSQLSSNQPTERALDYENSDQKDSSEIRTIELLKKEIQVQKEENLKLLSKHTQYEQEISKFRKKLYEQEQETTQYKKKMEKYKLELDDCLNSNQQDKRYYIENFRPQGLLSSNTSQIIDIEQRIGVSPQLNCFDFNQSLSKFEHPVSYNNQSFKNPDEKNLTKLQLQITEKNNKIANLEKQINLIQHGSHSRGSSLTKFSQIQQERHVEQSNLMENFRFFLQHSQQKDKEVKQIKEQQNENFLKICRQLIYQNEQIQKMNSLIFDFQNEEQIKENSQNQNEDPQNLHQYYQQALNDKDKLLQLITTIFYENVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TZK9</accession>
    <name>A0A8S1TZK9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase</fullName>
            <ecNumber evidence="10">2.7.8.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0280138</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Functions in the biosynthesis of the anionic phospholipids phosphatidylglycerol and cardiolipin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>a CDP-1,2-diacyl-sn-glycerol + sn-glycerol 3-phosphate = a 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycero-3'-phosphate) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12593"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60110"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Phospholipid metabolism; phosphatidylglycerol biosynthesis; phosphatidylglycerol from CDP-diacylglycerol: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the CDP-alcohol phosphatidyltransferase class-II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.5" evidence="10"/>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8156116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TZK9"/>
    <dbReference type="OrthoDB" id="10250191at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008444">
        <property type="term" value="F:CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09135">
        <property type="entry name" value="PLDc_PGS1_euk_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09137">
        <property type="entry name" value="PLDc_PGS1_euk_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016270">
        <property type="entry name" value="PGS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12586:SF1">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12586">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--SERINE O-PHOSPHATIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000850">
        <property type="entry name" value="Phospholipase_D_PSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="12">
        <location>
            <begin position="140"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="246"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="254"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8156116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="540" mass="64881" checksum="04AE0D566F2099D6" modified="2022-10-12" version="1">MNQLLNNLKFHKPFFKLNTAQIEILKDPVDFYVNLHKGIKNAQKRIVWSTLYIGNGNMEEFLIESLSNQQRRFEQLRISLLMDYYRGTRRDKKNHQTPIDHYLYLRMQNLYSNNMKLGLMRHPFLPQFANMLEESAAREIFNVHHMKWYIFDNRVILTGANLQEQYFINRQDRYMVFHEANELADYLDDAQSALLQHAYYVDFDSNSKFDPLRAQPFKRQQYFKEFHQTWKIFKFSYKEAAEIKSDDEEYQEQQQEKVQKQEEDNNNNIKQEQEQEQEEELSKEEMLQFYERDLIVEQKIQERKEQAKLILNKSFNKGDVQNIINRLEKALYKDEERELDEGEECTVLITLHIPYLQTDEDKLVFMKILENLDQFTSVTWASGYMNFTKTIINFVNNCKTDIKFITASPEANGFHKAGKAKSFIPFLYRYFELAIDKPIFEYKRNGWTFHTKGFWAELQDGSIMTIIGSSNYAVRSEKRDNEIQAYIFTQCPMFKQDLKKEQNYLLNYAENITKEEIIKDNEIKINWKIKLLSKIFKSMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U4W8</accession>
    <name>A0A8S1U4W8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S acidic ribosomal protein P0</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0350038</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Ribosomal protein P0 is the functional equivalent of E.coli protein L10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U4W8"/>
    <dbReference type="OrthoDB" id="289442at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05795">
        <property type="entry name" value="Ribosomal_P0_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.105.20:FF:000001">
        <property type="entry name" value="60S acidic ribosomal protein P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050323">
        <property type="entry name" value="Ribosomal_protein_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030670">
        <property type="entry name" value="uL10_eukaryotes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039087">
        <property type="entry name" value="L10E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL10-like insertion" evidence="6">
        <location>
            <begin position="129"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="299"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR039087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8160541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="323" mass="35387" checksum="54EE416341E0CC40" modified="2022-10-12" version="1">MGKKETKDKKPAQFKKIYELLSKYTQVIIVGLANVGSKQVQDIRRILAKKNALLVIGKNTLFKKVLATRVQELPDTHEYYDELKKFGNAIKELDSLKNQVAGKVGFIFTDTPVFDLKPIIEENKVETPARVGAIAPIDVVIPPGPTGMDPASIQFFHALQIPTKIEKGQIQITKDFVVLKTGQKVGQSQAVLLQKLGKKPFLYGMEVLSCYDNGSILNKQQVSVNLNDIVTKFQQNVQNISAISLQNGWINEASAPYVLANAFKDLAAIGLQSGFVFDQIKQSNAPVAAAPVAAKVEQKPAAQQAPAKVEEPEEDVDMGGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U5E3</accession>
    <name>A0A8S1U5E3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0310041</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8157866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U5E3"/>
    <dbReference type="OrthoDB" id="300165at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8157866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="76" mass="9016" checksum="39476E2D4ABCC184" modified="2022-10-12" version="1">MGCLQQKISKKEQLLEFPIQNLSNGPITMNKKIILYHIQCYNRKYSLKSIDQSTILKRRRTKEQITEQSSPTKIYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U5Z2</accession>
    <name>A0A8S1U5Z2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Centrosomal protein 20</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">FGFR1OP N-terminal-like protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">LisH domain-containing protein FOPNL</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0340136</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in the biogenesis of cilia. Required for the recruitment of PLK1 to centrosomes and S phase progression.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Microtubule organizing center</location>
            <location evidence="3">Centrosome</location>
            <location evidence="3">Centriolar satellite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic granule</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8160068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U5Z2"/>
    <dbReference type="OrthoDB" id="5970631at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034451">
        <property type="term" value="C:centriolar satellite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030030">
        <property type="term" value="P:cell projection organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034453">
        <property type="term" value="P:microtubule anchoring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.960.40:FF:000002">
        <property type="entry name" value="LisH domain-containing protein FOPNL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018993">
        <property type="entry name" value="FOP_dimerisation-dom_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15431">
        <property type="entry name" value="FGFR1 ONCOGENE PARTNER/LISH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15431:SF4">
        <property type="entry name" value="PROTEIN TONNEAU 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09398">
        <property type="entry name" value="FOP_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FGFR1 oncogene partner (FOP) N-terminal dimerisation" evidence="12">
        <location>
            <begin position="50"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00055043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00076755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00081996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF09398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8160068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="165" mass="19091" checksum="3DC43D556C2B6CE0" modified="2022-10-12" version="1">MTTINEMKDALKETLESRGVLSQLRARIRAEIFNALNEDPEDKPKLSMENMIINDLIREYMEYNHYSQTTSVFSSEAGMPQEMLDRGFITKKLKIVEDNNSKQTPLLYGLVFGMKKVINNQEVQNEPQTGPKYRNVYPESGQVVESNNEFEFQGGRRNISDQIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U609</accession>
    <name>A0A8S1U609_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">40S ribosomal protein S15a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0280169</name>
        <name evidence="6" type="ORF">PPENT_87.1.T0310142</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8156178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U609"/>
    <dbReference type="OrthoDB" id="291200at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.30:FF:000001">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_uS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047863">
        <property type="entry name" value="Ribosomal_uS8_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003115">
        <property type="entry name" value="PRK04034.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S15A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00053">
        <property type="entry name" value="RIBOSOMAL_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8156178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8158066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="127" mass="14506" checksum="90188B8A49832ED3" modified="2022-10-12" version="1">MNVLNDCLRSIVNAERQGRKQVLIRPTSKLVVKFLQVMQRHGYIGEFEIVDDHRSGKIVVELLGRINKCGVISPRYDVTLGEFERWANNILPARQFGCVVLTTNVGILTHEEARQRHIGGKILGFFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UK51</accession>
    <name>A0A8S1UK51_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0420228</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UK51"/>
    <dbReference type="OrthoDB" id="309856at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.60.10:FF:000032">
        <property type="entry name" value="Glycine-rich RNA-binding protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000950">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051186">
        <property type="entry name" value="RRM_HNRPC/RALY_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13968">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13968:SF26">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="7"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="93"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="103"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="142"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="164"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8165468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="192" mass="22379" checksum="28DDBD041688CAEF" modified="2022-10-12" version="1">MGEIVPNQLFVAGYSRNKVTDERDVKEIFRKYSSVKEVAYKGSYSFVTFNNETEAQEALKETNGMSYKGQKLKVDIVDKGKSRKTGPSDSDLCFKCNKGGHWARNCPNSRSPRRSRRYSNSRSRHHRRRSDSRSYSSYSSSRSRRKNRYYRNKHRDSRSPKRQDKPKKRSISRKRSPSNSQSSKRANSDSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1ULU1</accession>
    <name>A0A8S1ULU1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0410164</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1ULU1"/>
    <dbReference type="OrthoDB" id="446382at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8164699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="162" mass="19369" checksum="4F5DB2A8C3A4D270" modified="2022-10-12" version="1">MCIKQIQILIIIYKCLYNVYSNYKVISLKLHKETFSLYNILFLILQCIIKFQNAASNIIDKEATNEIHIYILFRNFNEKIYLSFSWLNFATMIPAIFMLLLITICTCQIKLFQSLIKRNIRYQEDEIFDEYNGEEGFECSICMEELKQMEKYVELATLQSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UM97</accession>
    <name>A0A8S1UM97_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0410249</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UM97"/>
    <dbReference type="OrthoDB" id="302810at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="116"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="235"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="116"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="180"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8164859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="351" mass="40806" checksum="6A6BEEC8F15AB9C0" modified="2022-10-12" version="1">MNYNNTIHRRAQSNNDIQQVICARIAGLVEDEYQQQKLDPKRINLGSTLFQIPEQNDLISSVEQTTESSKQHSQMIKDLNISNVKPNQQQDKENFKNLSSCSTLINQIQQIVKSGSNTKELQSKKPFTSSNDATQKKSSIQSQMSFTKELQKKQGSNYLQYQQGQTLKQNITPDRKRGPSYSNKQTNQKQPNDSTPKQISGTDTLKSNFSISLFNNLIRQDSKKKISNSDICDSYRNIEIKMKKIEKEINTIKQRQDQLDDNNKNIQDQLKEFIYENKQQQEYGTKQLKKIEQLEQITRRNEESICYLKNFVVNSKKNDQNNMNFGNIQQSDITDLQKKFADFKPINHKVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UNN6</accession>
    <name>A0A8S1UNN6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0440265</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UNN6"/>
    <dbReference type="OrthoDB" id="2266637at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8166670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="128" mass="15034" checksum="789330A01C81920F" modified="2022-10-12" version="1">MQLSIKLILVCVNRRFSLGNTSKSAHKQWDVLGNFKESTQRQSSLKYLIGILGDDCILQFQLFSRLGRPIYFRTLQYQQSTKLKFIIKIKSLSRFWIIVVSQYPQIGQVCLFTPAYRPELNAIEHMFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1URL9</accession>
    <name>A0A8S1URL9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Leucine Rich Repeat family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0420040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1URL9"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052394">
        <property type="entry name" value="LRR-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8165096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="917" mass="106705" checksum="21A11DAFBA33262F" modified="2022-10-12" version="1">MHVGLSNKFSIQGSKRKIASLQFSDKTVEDYSKAMSVGNPSDLNTSPFPQLYSPAGKRQMFQRVDRFSVLQPIKKEEDKLTDKDIMRQLQESPEQKLEDQIPKQLVGSQSARSYFQHYKVLNKVKQQNDYHKINDSIQTQMLKQAESLDVLPCKLGLIKLNGNESQVSINNHHYGDKYISVLSEGLRQNQGIKEFLLSNNRIKQDGAVTILNQIGKQATVIDLSKNDIGQLGVDCLCQQLQMRENKMEVINIEDNKLGDKFVMKILKCLLNTQNKVKVLNISKNFLTNEICDLMKEIILQLDLLEELYIHWNQIKGSGGQKIFEALIENKNMVVFDGSWNGFGISEKSNCTQKVCDFLSQNKVMLHCDLSANQFTLQDCKLIANSLKPNRTMYGFHFAGNWGVVDPRGFLIIDDNAQQKVLGETLRIKGLEQVTTLRHEDVCWICEGWQEQVFDWIPDGECDPLFLHMNFEEYKQVYIPKRNDGTYKLPLMIPTGQTQFLFTVIDNQTTAVNYDNIKFGHVIKLKVNGINVSCQLDNINVVKKLKHFQLMDKKEMKALTSVLPRTPDPIYIPPKLKKQKRIWSFPISIWFKDFRFENEEFLRKCFEKDWSCSKISKVVKNPDEFNEVKNLLWKDYKMIKETYKWYSSYNPSGDVWSISSNVITDFSSTTELVDNKTFKLSDLDLKFIATCAASIEYKGNYRNPERALCRYQFMEFLVRVSDDKYLKQKQANNMVDSVQMILDQCRPIMQQYNAQKWRDERYFNEQCDDCLKYYKTLLNYVYNRYSIKKVKPGQKKFMCLDELHEICGQAGLFDEKFVDRDADLAFNLSMMLQIDELESDRIFQMTFVEFMEAIARIAEKVSLPAAPDMSWEQRQQQPLHIKLERLLILLAQTCASEEYKQQFGNPQKSIFDVQPEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UZX5</accession>
    <name>A0A8S1UZX5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0500150</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the KTI12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8169079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UZX5"/>
    <dbReference type="OrthoDB" id="9972657at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002898">
        <property type="entry name" value="Protein CBG21524"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013641">
        <property type="entry name" value="KTI12/PSTK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12435">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08433">
        <property type="entry name" value="KTI12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8169079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="280" mass="32725" checksum="8C6AA5EE3C850D65" modified="2022-10-12" version="1">MPLLLICGPPASGKTQRAIEIQKFIKEKMNKESIIINEENLHLIKDDAYKDNTSEKMTRGFLKSNVEKYIQAGQLVIFDSINYIKGFRYELYCLARAAKTTNCLLYLNVPLEICQKNNENRENKFNDNLLIDLYKRMEIPKQKDRWECPYFEIRFQEKTPLDEMAEVLFYAEKTAKDPIATKKEQQNEGNFVHEQEKRVQDILDQIINKQVESLQIGNGTIKFQGTKALLVLKRALSIIELKKLRLEFLNMLRITPVKTLEQTNEAFINFIQAQVERLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V078</accession>
    <name>A0A8S1V078_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trichocyst matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0500200</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8169179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V078"/>
    <dbReference type="OrthoDB" id="295118at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035861377" description="Trichocyst matrix protein" evidence="2">
        <location>
            <begin position="16"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="222"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8169179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="363" mass="42321" checksum="C8A68D5E84C092D5" modified="2022-10-12" version="1">MKIFALLLILVIVSGTNHQQRLNYTKQRSITSVMAEVEMKIKSHSPLDAVLNILTLFRDSVNEEQVNHDQIFNVELNECQSEYDFRNAEIQNSKNTFRDSNVQLNTCQYSKQRTIDQQQINQQQENTFQQHLNTILQTADSEQGYFKKRNRQYEDSLNSIEEALTILEGISFGYRSFSELSKVSQRMLQTSFDINKTSIYAPIFNAFIQLANQGESIDLSLLQQVEHLLNDLKSTINNAYNQFTESNSQSVALFNLQKEKVNKVLSRLQQQYEKQQIKLDKLQTCIGVQQAVSNSAQSKQQRNQQLLEQAQALCSTFQTEYNYGTQSRRNEIQLVNQLEQMIRQRFEEVHEEKIVLQFAKLKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V1H7</accession>
    <name>A0A8S1V1H7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0490200</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V1H7"/>
    <dbReference type="OrthoDB" id="288610at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8168606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="340" mass="39772" checksum="065753B9277FE7AE" modified="2022-10-12" version="1">MLTYVNENPKFAETLKQLVECGKDRAIRWPEIAKVLENMFSITISKPCLLKPAYQSIISPNVELDKQQHRLLVTTVIKHSAIIKQALPDFQKTSGIQIQSKKFQSRMYRSFKTAIETGIQVFVQKKSEYRKRLVRVPGSAISRVLQCKVLPSDDSFTNKLKFMSDQLSLLLMISIDYDGEDITALYSTVTQKMTKKNFHLLLNCISFMFDLKCISQGQITRDDQLTPDYWLQPTIKENQKLYVYNYFFEDDNCQKYKMYSDAVDVQQEMEYKVFLNINPISQPKQQSFLKKNAKLKTVRGRITVNDKKYPSERTIIFAEPEEESEEEFYDIKFGRKQNCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V367</accession>
    <name>A0A8S1V367_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0540207</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8171264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V367"/>
    <dbReference type="OrthoDB" id="291330at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="256"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8171264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="284" mass="32364" checksum="3118AEF2FD83564C" modified="2022-10-12" version="1">MLPDTNQQPQLIEEYGGFPGSKSFSILGLLTLALVYAKMTVHFYVKWTVILSPMIFFFSYKFLKSIYNISQQSRKDKNQSLKQRKIIMLWNLCQLLITAPLAVSTFYLGDLIEKAIQQQDIAEPLTHLFACFAFTLLVYLLYSLSSQKETSQNQLGNKNIILNFLLSMFGNSVSFCAGGVCNSFYISTLSAFFSAFGIPITQYLHYLNYLCIILLAFSLLSLYSVKESILYAPFLITLIGSSLIVNEMFFYKLPCAIWIGNGMIIGSAFWNSKLNQFSFFKRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V511</accession>
    <name>A0A8S1V511_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0550048</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V511"/>
    <dbReference type="OrthoDB" id="306440at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8171483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="206" mass="23632" checksum="632D116FD032FCF3" modified="2022-10-12" version="1">MGSSHCCSLQTDDKSEIVLKIEDTEKSSLRSSKKSPKKKQETPSQKEKLLVHPNKCEDPATPGFADLQSFGSKHSEQSYKDIQLVAEIVMNLEDIPKKIEDIQQSPITSQRSLKKRRKNDSPLCDFQPKNSLKSFDSKKLNKLKSLSEHRVKFEEKSENDSNRSNSADNKSVKSIMKQELKYSQFRKMQTQGDAESRKKVHFQLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V9B0</accession>
    <name>A0A8S1V9B0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0570059</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
            <location evidence="1">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V9B0"/>
    <dbReference type="OrthoDB" id="300500at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46613">
        <property type="entry name" value="RADIAL SPOKE HEAD 10 HOMOLOG B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46613:SF1">
        <property type="entry name" value="RADIAL SPOKE HEAD 10 HOMOLOG B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0282">Flagellum</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8172372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="301" mass="34399" checksum="3299D7048A68829D" modified="2022-10-12" version="1">MGCVCNSNTSKTSIQSTQISINIAEQYKNNKNIKTQDSLEDLSQSLCNIGQEITSLGQLRPEVQEQINELGTFKAIEQMDTNTSNYQYLLCSDGSIFYGSVINGIRNGIGKQHWLTDGNYLESFWQNDKANGPARMIYSNGDVFEGHLIDNKANGFGVFKNKKKEVRGYWSDNRLSGNGIEIRKNGIKYEGQFKGGIIDGRGQFTFPDGRKYCGQVQQGLMHGQGLMTWPDFSYFKGEFRNNHFKGYGCYTDIDSQTYFGYFFSVYHQDIKQMEIFYSQDQNIQECNKLKKFKKIYGGEQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VA76</accession>
    <name>A0A8S1VA76_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0590147</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000059">
        <property type="protein sequence ID" value="CAD8173481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VA76"/>
    <dbReference type="OrthoDB" id="48314at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF263">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="119"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="814"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="816"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8173481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="876" mass="100946" checksum="1AD28F87A5D1FEBA" modified="2022-10-12" version="1">MNQSTGQNQQSQPLINYGNVKYSFKCLRRHLLKDKVYHVLALQNMITIGEHLNDPNPKYKLELNLKNTVYWKQDHGQNKAFGIKYKKNVKYFEASAEQLTKFREAISCFIGFHSIDDHYKIEKMIGSGSFSQVHKVFRLHDDKAFAMKQVSNQQKTDKENMQLLENEIGILHSLNNEAIVKIYEVFRIEENQYGLIIEFVDGICLATLIEQLKKNQNQLKEEDIKILLKEILKALNFIHQEQVIHRDIKPQNIMISQSSFKNVKIIDFGLSIKNSQQYNRCGTPGYMAPEIVNMKKEDQKPWTSLCDIFSLGVVFYKILSKGINCFQGQTSDQIVANNKKCQIDWSNVQQFNYSQNCISLLKAMLAKDPEERISADQALQHPFFIDLPPVLETDFAGNDMTQQSKGIVNQIIDKPNLDSIAESLNDLSGNQKQHIYVGEFKPSRISKENSKEFSDFSILKTKNSETQVVQPIDNPPPIKFTQQEAEEIIQKFRVYKPQNKQFKYENLLKFQNCKLKIYKDSYYFGQLVNGKKNGFGVILSNNGRTYEGQFENDRKHGSGFERFPDGASYNGTYINGKPDGVGKFLWANGEVYEGGWQNGLKHGQGIWNGIKGDSYVGEWKMGNPEGYGVHVWVNGDRYEGEFHNSLKHGEGIEYLINGDIYKGQYVNGKPEGVGEYQWSSGSYYNGTFSNGLRHGKGLWIKDRNATLTDNYEGEFVNDKKCGYGVYKWAKGSKYEGNFYDDLRHGYGSMYWNDGSYYIGMWEQGYQWGEGEYCKKGEQPKFGTFEKNLLVNEDQEKLSQYHSKLPPQIRIRQYTTSQRSVRSNSQRPSSQSQQRNVRPSSVKSVNQIKQGSMGNNQFLCKKRRQPSLTKQTFDLDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VDY5</accession>
    <name>A0A8S1VDY5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0610218</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VDY5"/>
    <dbReference type="OrthoDB" id="300876at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="209"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8174663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="300" mass="35449" checksum="B181E3988106C780" modified="2022-10-12" version="1">MEQNTKVKQEYFKKLKLIQKKMENIKADQSPSVIKYMVRRKSAEEVQTPLHDVEFNQQPKKKKLQFQKTFQKVVIDPIIKLDEITEEKWAIMSNNYKSLIEKSRKSISKHSQIFQPPLATKEHHKFQFLTRRQSIEFRLREITTAPSNHSSVSPIKINTNTQLADPNYDRFKKYYQKIGIVADNPVQMKFKQLISTPIAPQKRKEKSQHSCNNSQQNSSSSPPPKTRTIHIKPNHNSQYLQEIKEICSVASNYQTHVKQDERIQIHQTSKQMGYIQAEFNKFHNMLTTDLERLDFYDEKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VM81</accession>
    <name>A0A8S1VM81_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Jacalin-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0680223</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000068">
        <property type="protein sequence ID" value="CAD8178017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VM81"/>
    <dbReference type="OrthoDB" id="309140at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001229">
        <property type="entry name" value="Jacalin-like_lectin_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01419">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Jacalin-type lectin" evidence="2">
        <location>
            <begin position="32"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8178017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="339" mass="38788" checksum="2EBFCC5995591293" modified="2022-10-12" version="1">MSNKVPPLITLGVDRQGLQKFDDKIQLGNEEFKISGVKISLDDNQICGISFTYQNIQNGRKIICNDTCITGKNISQYEFEIGQNDFIQVLFGYYESGITQLGIITYKGQQVIFGKDQGYKFNYMFMGYTFTGCSGSYKKGSIESLTFKVAKLPKQFIIQQFNPLLGILYPENPYESIILTNFDQEQKQQYEQQPQPVMQPVVTSEIIYSMIPNQSPQTQQGYPIQNQQVPPNQIPPYGNPYQQPNQYQTYQQYPNNQQYYSTPQQGTNQVIIVNDQQQPQQQQYQKQGPGFAGTMARTAIGTFAAIETVRMIENIGHHHHHQANVVVVGNKHHGHHHRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VNJ5</accession>
    <name>A0A8S1VNJ5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">mitogen-activated protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0650021</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000065">
        <property type="protein sequence ID" value="CAD8176316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VNJ5"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07852">
        <property type="entry name" value="STKc_MAPK15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000238">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000166">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="17"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="352"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="357"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8176316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="547" mass="62505" checksum="10BA3B36423272B3" modified="2022-10-12" version="1">MENDKQSQFEDHILQRFTLLEFKGKGAYGVVWKAHDKQTKSIVALKKVFDAFHNKTDSQRTFREVIFLEQVSKNANIIKLLQVIKAQNNRDLYMVFEYMETDLHKVIRANILEPIHKKYVVYQLLKAIKFLHSGDIIHRDLKPANLLINADCLVKLADFGLARTVNTSSDDDVPILTEYVATRWYRAPEILLGSQKYSKAVDMWSIGCILGEMILGKAIFPGTSTMNQVEMILEILGTPTEEDIKSIASPLAKHIINDFQYVKPKNFKTVFSQESDDTLSFLKNLLVFNPQKRLTVEQALAHSYMAEFAGSEDETVIDFPIQTFMDDNVQYSIEEYMTALYQHINQKNTQQVQQQNWFSSHQSPANASKPSNTLQQSTTPTSALDKRKKKGDSIEGFNNNSENKQIIKQEVKKKVSEDNQNQKMSAFEKFKSIENMIKMQKAKLGTSNGQITVQNSSKLQYGNKSVNITRAESLKKKEASCELLVPSPAAKSKIQKIVSQNSFKDPQKIQIIKSQSKLPKHESQHDKFLNKKTYLQYILSGKAKLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VRM5</accession>
    <name>A0A8S1VRM5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0720112</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000072">
        <property type="protein sequence ID" value="CAD8179577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VRM5"/>
    <dbReference type="OrthoDB" id="296964at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035868023" evidence="2">
        <location>
            <begin position="19"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="250"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8179577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="358" mass="40989" checksum="D1B63B9E5EC4CA36" modified="2022-10-12" version="1">MKIAILLILAVSLNAVSTDLNRQAQKHVDALMKTNWGQTILQLAELHAHTGGVLQDLVGAIEEMIQQMQDELDEVEYNYGVRTNEHNSLSLQYTQEVQDADIDIQRSADTLENLLYPRREQLKGKIQQIIDYQEFNRKTVDEETLIREQEHEAFEAQIAEFNDAVGATDDALNLLSTLTNPSLVQIQKFQNNLRKIEARIKPHSQHQTLIKALITLASEQNFSDQGIIKQIVDKLNEFRNAVVDAINAATAQEAQDVQDYEDRIEQLDAEYAEFQRQITKITIDLNATQEKIEQFLSFQAQRQSDRNTAQALLDLENEQYADDTQIYTDLKNKLIRDIQVTEEAFSLVKSVDFSKIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VWL2</accession>
    <name>A0A8S1VWL2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cyclin-dependent kinase 2 homolog</fullName>
            <ecNumber evidence="3">2.7.11.22</ecNumber>
            <ecNumber evidence="2">2.7.11.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Cell division control protein 2 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">cdc2-related kinase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PPENT_87.1.T0740003</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>[DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10216"/>
            <dbReference type="Rhea" id="RHEA-COMP:11321"/>
            <dbReference type="Rhea" id="RHEA-COMP:11322"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.23"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">May form a complex composed of at least the catalytic subunit CRK2 and a cyclin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="3"/>
    <dbReference type="EC" id="2.7.11.23" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000074">
        <property type="protein sequence ID" value="CAD8180232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VWL2"/>
    <dbReference type="OrthoDB" id="413582at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF495">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="9"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAD8180232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="338" mass="39656" checksum="047895EDB8BB5140" modified="2022-10-12" version="1">MQSIDHEHYTKVKKLPGGAYGKIYLCKCEKPIHNKRIFQWIPQLVEIETHPQFVVIKKFKPLKKRVGLDIDSLREVRFMNTMIHQNLNIPKHVFMKTSKKRNDDTFGSICFVYDHMVSLFEILSVFRESNESIQESDIKLMLLQILQGFEELHQKMILHRDFKPENVLVTKDGILKITDFGLARLWEDKPMTTQTCTMQYRSPELFFNAQKYGPALDVWAIGCVFAEFYLKQPLFSGESEIKILSKMVNILGNPTEKNWPGFLNLPQVVQFEKRDSMNLFKLLPKMSNEGIDLLSKMLQYDPNKRISIKDALNHNYFNQIESQNLSKKLMQIIYSKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VWV9</accession>
    <name>A0A8S1VWV9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0770093</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000077">
        <property type="protein sequence ID" value="CAD8181830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VWV9"/>
    <dbReference type="OrthoDB" id="305219at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8181830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="322" mass="37876" checksum="754BD6FB03164A38" modified="2022-10-12" version="1">MHTMTQLQLSGFSFFSPRNIRDNQNLILKTSTTTHTKQRNIIFPKKKKQKSKSLPPSCRIPQLEDDQYLTTMTLTERIKKRYFQPLKNAVAKITEIRQQQQSRRKSTYGLMFGQLTSKEMLNLPTIIEQHKQVNKQELALASFAIMLERKQRRKTTRKLSKKLSKQESVIEYDNMERPPSCKTPPKKILICKPKIKSKLQLENERYFKGFDLKMCHFLSNKLQKNVIPQQNLIVKSHFQTKSCVSLQNLQICSFVSSPKIKAEIKQNTEVNLILSKQKSKSTLKIERPYIKHNTQLLKKIQQTFETKNTTWSKQNSIEQKQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VX18</accession>
    <name>A0A8S1VX18_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0750019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000075">
        <property type="protein sequence ID" value="CAD8180765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VX18"/>
    <dbReference type="OrthoDB" id="291563at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8180765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="601" mass="70518" checksum="85BA026670FBB5C3" modified="2022-10-12" version="1">MNQQNIIEFYPNLSEQFQSILLNLYRNETLQQYHSIIQQIKTKFPFPKQMPQFMYPNAQQISEFFLQNYQPIILDLTSLSLKPNVKIKLYKNAVYFGEIHQEMRQGQGVLIKENNQIYEGYFALDKKDGIGFEILKGDTFYHGHYVQGFPHGEGFYKSASHSYIGQWQHGKKSGIGWCIGNKNDYILGTWENGKFYGFGLQIYDSIYFGEVINDQKYGYGEEYFPEGDIYKGQYKNGLPNGKGKYIWKNGNQYQGDFQNGLRWGEGFWEQKTEKGIQFYKGQYVNDKKNGHGHFHYSNGTEYIGEFNEDQRNGFGEIMWPERATYKGQWKQGLMEGEGVYQYQNHKLQGIWKQNQLISKEKVRVSLNQFPLQHKLKDIVEDEEIQSRVAEEPDHEKIGDIFKKITLQSKTSLSVSDTSKFPQLIQVQKQQDINQYGQACSYPTQRTNQTFSIGIQTEINSTKKKLLTELPSIQTKKKHVLLNYSIDNKIKNNKQNLSESVTNTNSPETRESELKNNIIHQSQEKKQRKVKQRLSLLTKIEEKVLKIRIEKQKLSPYKFEKLWSKKVHRDPLLYQVVNQAKSLIYPPIWVPPSLHPQIKRKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VY78</accession>
    <name>A0A8S1VY78_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EML-like second beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0750213</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000075">
        <property type="protein sequence ID" value="CAD8181125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="674604at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="65"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="107"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="114"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="149"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="191"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="233"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="275"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8181125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="380" mass="42392" checksum="58A69CDE150171E4" modified="2022-10-12" version="1">MKIQKKFLNLDRISLRPNLIPIYQNQFQNQLLYFFQYLLKQNELYNFIFNITYSXAKTGRQKAILEGHRSAIQSVCFSPDGTTLASSSIDNSIRLWDFKTGQQKAKLDGHSSSVWSVCFSPDGTTLASTSIDQSIRLWDAKTRKKKYKLIDNDGPVYSACFSPNGLTLASGSWDNSVCLWDVETRQQKSKLEGHSYAVNSVSFSSNGLTLASGSGDNSIYLWNVNTGQQQSKLDGHSDFVNQVCFSPDCTTLASGSGDKSIHLWDVMTGQLKCKLEGHSGRVLSVCFSPDGTKLASSSYDKSIRIWDIKAGKSTQFSERMNTNITILLISQQITLSAQGAQIYKGEFMSYQGVDLRTFLEQRGGYLIESLDDFSSHPVQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W375</accession>
    <name>A0A8S1W375_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0820023</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000082">
        <property type="protein sequence ID" value="CAD8183870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W375"/>
    <dbReference type="OrthoDB" id="10340050at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8183870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="94" mass="10996" checksum="67F1708A042134F6" modified="2022-10-12" version="1">MFSVLGTFFCLLCGSRKERKLLLLTIQDQNHNTYYIKAKPNMKIEEIKSKCGIESERSSLFLEKTLLEDDAKLKEYEITSDTHLTLLLHEEIEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W4Q0</accession>
    <name>A0A8S1W4Q0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0800063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W4Q0"/>
    <dbReference type="OrthoDB" id="10478307at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8183069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="165" mass="19812" checksum="1A2241B4FFA33883" modified="2022-10-12" version="1">MKNEIKKYKTKHTLQNLEKKKNTELQSVLILSYPDIGITQFKIKFFLFTQKSGKIKYYQDTILSIHSYKLDDDIYVCLLFFTLLKPLFNLFVVLFIIYAQTILFYYIQDPHHWKKSQPPQPYQPTNPSTIIMYSNYPITIINPSIMHNIIFNHVHFYFQNNESML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W9E9</accession>
    <name>A0A8S1W9E9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Piwi domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0840222</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8185202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W9E9"/>
    <dbReference type="OrthoDB" id="445936at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04658">
        <property type="entry name" value="Piwi_piwi-like_Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Piwi" evidence="1">
        <location>
            <begin position="156"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8185202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="483" mass="54806" checksum="F7D5320AB4BC236B" modified="2022-10-12" version="1">MKQLSTVTKPRGVDRVKQADQFIQCFHNKESEELIKKWNIQLEPKCLQIQSSKIKPGNIMMGNNTSINIETGNLDRDTQTAMLRGVGLENWGILYSDRDSRQAEDFMNCLRESIEYCKFQCKAPRTFVLHSNRIEDWIKQIDLIVQQSQGPQKVTLLLLILNGPKKNAPLYTDIKRYLINDCPIASQVILSSTLNQPKGKVKTICNKLLVQICAKVGGTPWGVSELPFTDQPTMICGMDVYHSTGKAKKSMLSFVSTEDEFFSKYMTQSIEMETGVEFSFSLCPVLVKSLQSFCGDRNGPLPSRIIIFRDGVSNSQAKTVIETEVAQFRQAIEQVKTEKNSDKPIKLIVLSVNKKVGAKFYAGERNLDNPPQGTLIDTEISNGKDDYYLISQRTTQGTVQPTHYHVLVNDLFDEPNILKKLQALSYKLCYMYYNFSGAIKIPAPIQYAHVCSNFIGDRFDPRKPQSLIKPNPILNQRRSLFFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WEE4</accession>
    <name>A0A8S1WEE4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0880098</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8187002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WEE4"/>
    <dbReference type="OrthoDB" id="2441647at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="113"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8187002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="200" mass="23516" checksum="70634CCCD106D08B" modified="2022-10-12" version="1">MSQKKNTKRIHSSDGSLSIEQLKTICPDQYVTYEIFDLAFKQMWSVVNKLEMLQLQQNSNDKLFELEKRILNLEDVIHKLTCITPVIPESLQQQISELTDTGKQILVAIPQKFEQIQNDIIEKDERIKSVEDACTVLTRVLHDVQTEYEDQKDQIDKMESDLFNIFKQFQTVLKLEQDYSNIQNEIQNITKAINEIYQFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WH42</accession>
    <name>A0A8S1WH42_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Carboxypeptidase Q</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Plasma glutamate carboxypeptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PPENT_87.1.T0910070</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="19">Homodimer. The monomeric form is inactive while the homodimer is active.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000091">
        <property type="protein sequence ID" value="CAD8188301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WH42"/>
    <dbReference type="OrthoDB" id="10013407at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070573">
        <property type="term" value="F:metallodipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03883">
        <property type="entry name" value="M28_Pgcp_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000131">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039866">
        <property type="entry name" value="CPQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12053:SF3">
        <property type="entry name" value="CARBOXYPEPTIDASE Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12053">
        <property type="entry name" value="PROTEASE FAMILY M28 PLASMA GLUTAMATE CARBOXYPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="18" id="KW-0458">Lysosome</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="10 21" id="KW-0732">Signal</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="16" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035806645" description="Carboxypeptidase Q" evidence="21">
        <location>
            <begin position="16"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="22">
        <location>
            <begin position="250"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00025833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00033328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="CAD8188301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="461" mass="51658" checksum="B0C150334E6CD025" modified="2022-10-12" version="1">MKIVIFLVLYLSINAYMLRNRKDQMKQQKILQIRETIKNPHSPLYHSSYNRLAYYVDTFGPRMWGSEDMADSIDALVEEIQSYGFDRVWKENLGEITSWKRGEESVTLFDPRQYPQKLNMIGLGWSPAGTIKAEVEVVHSFEELKSIDVKGKIVCFNYEWKGYSSGLQFRTNGPKIAEDAGAIGTIIRSVASISISSPHTGMTNYQDIKYPAVAITVEDADMIDRMRQRGQKVVIEIKTGGIQYKTTSDNILAEIRGSKYPDEILLMGGHWDSWDVGSQTGANDDGAGVLECLEALKILKHLGIRPKRTIRFIAWSGEEMGMANNGAQHYAKTHSQENHIVAFESDFGSTQPYGFGVTAGEKFTELVISLAQEYLTEIGVEKIYPNAGMSADSAALRSVTGTPVLNNKIVDNDTHDFYFTYHHSAGDSMWMMNADDMDDNVIAIASIMYLIADYDGLIPTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WMB2</accession>
    <name>A0A8S1WMB2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UvrD-like helicase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0940130</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="3156807at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039904">
        <property type="entry name" value="TRANK1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21529">
        <property type="entry name" value="MAMMARY TURMOR VIRUS RECEPTOR HOMOLOG 1, 2 MTVR1, 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21529:SF4">
        <property type="entry name" value="TPR AND ANKYRIN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase C-terminal" evidence="6">
        <location>
            <begin position="795"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="416"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8189645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2336" mass="277233" checksum="53A55A58D0A861C2" modified="2022-10-12" version="1">MNKYVFKSEKEYLKAKQKLYQDKLNLILTDIDQFYRIRLSGKQFLIRYFQYLMEVNERQQRGDNSSFFYEMANYFREFNIKQFFPKKIWYFNVTKSFINSLSNVSETDIINIFILLNLILEGKFNYYSESIIKVLQYEGGNVKFPFEFDYIIGFNFQGFLKLVIQVIPKLYPILNDEGKPLHFEFRHEIILVGLGPENQKNSVGYYEIDKYFRNQQTSPNFFANMKLVKEKINTSEIIPHATKIGIKPKSWKDILLPREFQIIKLDCQDYVYNETFKRWIPASTQILIDNKLARINQFSNNHFLIQKYSQQPSAFQLFKIIQNIPNYKVNLTNEQQEIISYNGDGLVIGRSGTGKTTCALLRLFTTDILFKIRSKLDQINYQTTDIQLNQQQKDCQLKTVFVTASPLLACQVKRLYDKLVQNIQDVINNKKRQQKNQMETEMQNDQEDLDQSTYQIIDAIKSDQEQQEYQQLNQEEEMDDQEIDEYEKEMGKFLKFSDITQFPIFVTLRKFLFLVDASLINSFFTIFGNKQDRSSQWHNEQFGQMKLSQNNEVIEKKLEQFNQKLMDDIDNSDYIQTKMPEVTQDIFVRIFWPKILNTIKSKCPISKCFDPIYVWSEICTKIKGHETSHEYPDRYMNYDNYYYMQGLPEDQCSFVYEAFQIYEQNKYQLGYYDLLDLVNHINYELAYGNDVIENVHYLLLDELQDVPKAVLILLDQLTELGLFCCGDNAQNIAKGISYKFFEVQNCLLSFRNSKKKMHRNLKIFDLSVNFRSHNKILQLANSVIRMIEIFFPYKIDKLKKETSNLKGPKPVVIKSDNVKHLLNNLCDFFSNDKQIVEFGCNQVIIVKDQDSKSKLPIELQNILCLTIYEAKGLEFDDVILFNFFHDSTASIKEWESLNDLEPQVEYLKKIDYEKYITNHQTEVIASSKQKNNELVEVWQLKMKNMKECQEISIDLCQELKQLYVAITRPKKRLIIYDQSLEKRRIIQNIWIKLNAVKIADCQIQPKDIRFQLLLQNNNKENWKRQGFKMFRMNNYYQAAKCFKFAEEKQLEQKSIAYNIVVNNAHISNSHQQFLQAAIIFEEIGLLPRAAQCYFTAKQFQKAQELYEKLGQINEMAESAFFAQNYQKAAQGFEILGDIRRSIDCYCLQKDWDNVIIMLNKYKQEFSQQERLAFLNQFFPNYLQNLAQQIQDKVIEQSDCTQEFQAINQEDKSILNQSESFIIQNSILNKSEEDYQVINNQIINQSNNESSLEILNNEEQSQSFQIENESQQNMEHLSIFDPEDEWLKYDQKQLIKSMSSKISHQSEFSNIVLLNQPPQITLLTSKNNIFISNKVLQELIYKLQLFSNEFKNHLDQQKYKSVQLSNRQDDEKEFDHIINFVYDLDNIDIDSIYMILDILEQFKSYKLCIYLCNHFKLATYLGRYLVSLMSTYSTLNKTKSQVNIQMITFGSIRRNLLEQSAISQIAINNILETINPTFLTFKNEEELSLNNSLGLSCYQQLIGLGFWKSFIYQLNFKHSIDLCMSFNNYSDAIEIFKKVQLNKKYELSEQEKFKLMKIKYYQQIKLIQGQQERASDFAKELDDIFQITKNYYMNKKQELEEKDLNQLIINANIHKKNLNFNQKLKQIEAVILSFDILKLRFECSYEIFIGLFEIVEYFHNQLITFQNQEDIKEALLICYGFSIPQGDIMTHYSHSLFLHISSKIVKQAVKDQQNKDDIQKSNSSLMFVDIGYEYISIHFEQAIELIKNSFSQKILMILNNKSKKSMDYYQVNDDDSFSVNSQEDWEDTLAELLVFLMLEQSNKKFQCKIIQKKEEQKKLHPLSIYYSKQTELQEYSIVDSEPSQILQLIQRIILKNNLRITNTPNYIKKLIRDISLNYIIQIKNQQDNSHSKMILAINLLNLTDQLSYAVLILQNSKKGNVPYLKYIEFLECQHFGIVEDVIGCFLDYSQFVNDKFYLDEQLYHVIRIGLNILISLLCNSQEKLTISKGYKEYLNNIENVDENNCKLETGIFQLTNQQLIEEYLQFLFEFYQYCCSQYYQDQIQQFLMIFAINLLNIQQVKTIKSLIEYIKDNQKLKNLQRLQDYLIKEISKNKQEIQKKRFIIITLTSYLDEDIIEVKILNTKSDQQLDECYDKCIQVWSVYQSETDSKINCMRDLIKKWKGFRQNHKIYLTEQIKRNLQFIRSLEYFKIGDCQNLRDLNRYMRQNINNFNEYLQLSVTLQRQLLKARQNFVNSLDIHLINSLLNDLGEQMKLLLKGNDIYKKVKQLEMDLKMWEKNDEERQTNEKMLELNKQLIIEKWKHLKAGIKINSNKLNQQLNSRGQKDQLTTIQEVVEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WNY3</accession>
    <name>A0A8S1WNY3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tubulin/FtsZ GTPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0960103</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000096">
        <property type="protein sequence ID" value="CAD8190407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WNY3"/>
    <dbReference type="OrthoDB" id="271881at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02187">
        <property type="entry name" value="beta_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013838">
        <property type="entry name" value="Beta-tubulin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00228">
        <property type="entry name" value="TUBULIN_B_AUTOREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0493">Microtubule</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="5">
        <location>
            <begin position="49"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8190407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="440" mass="51476" checksum="AB6767C65C679C1E" modified="2022-10-12" version="1">MREILVLQLGGFGNKMGVKFWEEMFKDSKFEIDNNLHEQSNILQLNTSNVVYYNLQQKIPLPRSIQVDLGQDLPFTNIELNPCNQFSFNYSSGNNFGYVKNNCCNEIVDIIMDSIRQEIEQCDSFQGFQIFASIIGGTGSGLSAVLSQTLKDEYYDTIIQCNLLVPSIKQNDVIVVSPYNSGLALKHLMDSAEQLIFFDNEGIKQNLSRIGIQFDYQDANLHIAQTICCNSSPFRNPGDINSCLRKLSTNLIPFPDYKLLTCSMTNNQQSDYYNEYQKMDEYQIFKELMSTDHNSTITDYFQGRYLAAALSIRGEISNKEIYKYCSNFNYFLEDKDYKTRARKRFEGVNMFFTSICKRKFQNYEFFGSSIIHSNSINQQLDNLSEKFKKMYLKKSYLHKYIQEGIDDQEFVEAQSRLDDIRYQYQINKDRNQWENQEYWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WWX3</accession>
    <name>A0A8S1WWX3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1050115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000105">
        <property type="protein sequence ID" value="CAD8193932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WWX3"/>
    <dbReference type="OrthoDB" id="312708at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8193932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="122" mass="14730" checksum="7890DB558DF99866" modified="2022-10-12" version="1">MNKINLQKKQKQKQKFIPLGFVIKYDPPVIGLLYKRSIQENKKKVYNIHLQNLIKLDDEVEITKQLFEEHPEFLDPDIIEPEQVLGLVQRLIEFRQIMERDDDDEEYQDEETDSHIIDEMFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WZF2</accession>
    <name>A0A8S1WZF2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4485 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1090037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000109">
        <property type="protein sequence ID" value="CAD8195293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WZF2"/>
    <dbReference type="OrthoDB" id="78101at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027831">
        <property type="entry name" value="DUF4485"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14846">
        <property type="entry name" value="DUF4485"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4485" evidence="1">
        <location>
            <begin position="9"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8195293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="180" mass="21364" checksum="FF95B99593BEA93C" modified="2022-10-12" version="1">MNSPRAETLDQELSEAINKIYNGCQKINAKHLKIRILQWLDKVRTPTHNFIWKQNSLLYARVLLEMTLEQQLDKPFRGVPPDGPLPRLDKFDVPQHIWQKIKQINIQQPMSPRKPLGMLTNRNFTPKLKTEPTQYKSNKFHNSQKSQKWDQIKETLEGANRNMKEFKQFLENIQTEKLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X623</accession>
    <name>A0A8S1X623_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cystatin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1130070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000113">
        <property type="protein sequence ID" value="CAD8196641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X623"/>
    <dbReference type="OrthoDB" id="302404at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035811553" description="Cystatin domain-containing protein" evidence="1">
        <location>
            <begin position="16"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8196641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="123" mass="14365" checksum="3ACF6110B7D0E0A9" modified="2022-10-12" version="1">MKIVLVLFFVITIQSERLLVSYDNGFVDDDNDFAGGVFPITDSNSDNYNKALQYAQKHYAESCKLNDLQWESLDGSKNQMVQGMLYYFNVTMKGNEKQEKYEIKVWIQVDREQTIEITQCKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X877</accession>
    <name>A0A8S1X877_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T1110118</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000111">
        <property type="protein sequence ID" value="CAD8196096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X877"/>
    <dbReference type="OrthoDB" id="9332038at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14210">
        <property type="entry name" value="PKc_DYRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF22">
        <property type="entry name" value="DUAL SPECIFICITY TYROSINE-PHOSPHORYLATION-REGULATED KINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="299"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8196096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="624" mass="73592" checksum="C5EC8279332724CD" modified="2022-10-12" version="1">MKQLSLLNLINIQPKQNATTLYSTKNSTKKIRSFDIIGKSPYKINPARQTQKQLIKEYQPITSRATQRQNIKSPPEITKSNKPISFSNHIDLHQNETQTKGQQKSYHLQLKSILIKEQDQIHLFDQAKRINTQITSNLIPQITKLHLRTHPEQLDQETSQKLQPILHNTNLSKSEKLLHLEKNQQKFLDLQNKITNNIKQRNQTKTYSLTTHQPQTTIKQIADQFQLPQTTQIILQYFQDQLSEFEKKEIIDYETIYYLAPKNIATAQRDPLGNLFNHGYDNQHGDYRFIKHDQIAFRYEMLEKLGNGSFGYVFKVFDHKHHKEMAIKIIKNREKFYQQALIEIDLLKIINKADNSYCLIKLMNYFEFRNHICMVFELLCCNLYEFVALNNFVGLEPDLIRRFAIQILQALLYLKACNIIHCDLKPENILLKEFTKSGLKVIDFGSSCFANNKMYTYIQSRFYRAPEIIFGINYTFQIDMWSFGCIIAELFVGESLFQSKSEKELLYAQIKVLGMPPKEVIDQSPKRSKFFDDKYQLNYKIKENEQISQLKPLSQLLEKADTDFYDFISKCLEWNQYQRMTPEQALDHPWIINSLPQSIRNELLQKRTNVLSISNKYHDKQESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XAS7</accession>
    <name>A0A8S1XAS7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1170010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000117">
        <property type="protein sequence ID" value="CAD8197943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XAS7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8197943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="170" mass="20481" checksum="03AF5A4D8D06EF34" modified="2022-10-12" version="1">MQVMKQLKSQQSHHYWMPYTIYINEQVLENKIRLREDVPYFSILSQWHFIQFEYGLDKKGQRSQFKISYFDNQDLKSKQYYLGNSQYYSLFINTKFYAYFGGDFTIYQKLKGQLAGFYFISNYPHDLDFLIFCHYSCKTCNGPQISDCLSCHDNNERVYLEDIQQCVCPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XF42</accession>
    <name>A0A8S1XF42_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1220006</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000122">
        <property type="protein sequence ID" value="CAD8199518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XF42"/>
    <dbReference type="OrthoDB" id="319282at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8199518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="70" mass="8393" checksum="762D32C9FD91A1A9" modified="2022-10-12" version="1">MQLYWNIFFIQGDQFKFQKEIPFVLKSILMGLNIIVQLNGQNYYAGVLQEFSSSQTFLNRYQFPKVVYQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XGL9</accession>
    <name>A0A8S1XGL9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1230064</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000123">
        <property type="protein sequence ID" value="CAD8199998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XGL9"/>
    <dbReference type="OrthoDB" id="285621at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8199998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="127" mass="14996" checksum="8B550D5A1F4C8112" modified="2022-10-12" version="1">MINYTRTINIDDTTKHRLSTQCKDNFLQFIMLQKAGKQGDLKMLNHLKTVYPKELKAKIQKNSTNIDYLKHHEQSEKILRQQAKFILTSRKNNNLTDRPRSRLDELIKRPSNLSPRCLLTTVDKFNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XJ09</accession>
    <name>A0A8S1XJ09_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed RNA polymerase subunit beta</fullName>
            <ecNumber evidence="8">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPENT_87.1.T1260030</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="8"/>
    <dbReference type="EMBL" id="CAJJDO010000126">
        <property type="protein sequence ID" value="CAD8200867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10248617at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.150:FF:000034">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007646">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007647">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04566">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04567">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNA polymerase beta subunit protrusion" evidence="11">
        <location>
            <begin position="17"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="10">
        <location>
            <begin position="172"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="12">
        <location>
            <begin position="425"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="13">
        <location>
            <begin position="524"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="14">
        <location>
            <begin position="608"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="10">
        <location>
            <begin position="662"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="12">
        <location>
            <begin position="829"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="13">
        <location>
            <begin position="928"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="14">
        <location>
            <begin position="1012"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="9">
        <location>
            <begin position="1060"/>
            <end position="1358"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8200867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1359" mass="154836" checksum="D0E531ACBCDA2D59" modified="2022-10-12" version="1" fragment="single">MITEKDKLITSYLSFRGLQKQHIDSFNYFIEQRLEQIVKSPLNQRITCESDSSFLLEYLSIRVENPTFTSSWDKYSLKSLLYPHECRLNDQTYAGNIYVNVRIHMKGREPIEEKDILIGRMPIMLGSKKCNLSGKTEQEIFQYGECPYDPKGYFIIEGNEKVILILEQIVDNRIIVDIDNKTDQYVSTVQSYQLETKSKASIILKKNKFYVKSSSFKDAPLFIVFKALGIVNDKEIIELIGTEPSIVEKLLMSFQDTSDEKVKTQQDALQYIGKLITSKFGGQSSNQAEQAREKLAQVFLAHVNCKKYDFYPKAIYLGQMVRRLILAMEDKRFVDDKDYYGNKRMRCAGNLLELLFEDLFKGLNSTISSILKKELGKANCKFTPKDVTMKMKEFGVDITKGLNRAIKTGKWTIQRFHMDRQGVTQLLARISYTSALGMLTKMRSQVQKTLKVSGPRALVGSQFGMICPADTPDGEDCGLVKTLALLTHITQEDIQDSVYKIILTMGVEDICHFKPSEFHKNYIVYLNGIIVGMHARPQTFVDQLRILRRRGKINEFVSIHKDDLRKVVNISGDSGRLVRPLILVKDGKPQLTQIDMIDFKQNDAKTVFSNYVKNGKIEYLDVNESDNAFIALTINDVTIETTHLEIDQMTILSCVTGFQDTSDEKVKTQQDALQYIGKLITSKFGGQSSNQAEQAREKLAQVFLAHVNCKKYDFYPKAIYLGQMVRRLILAMEDKRFVDDKDYYGNKRMRCAGNLLELLFEDLFKGLNSTISSILKKELGKANCKFTPKDVTMKMKEFGVDITKGLNRAIKTGKWTIQRFHMDRQGVTQLLARISYTSALGMLTKMRSQVQKTLKVSGPRALVGSQFGMICPADTPDGEDCGLVKTLALLTHITQEDIQDSVYKIILTMGVEDICHFKPSEFHKNYIVYLNGIIVGMHARPQTFVDQLRILRRRGKINEFVSIHKDDLRKVVNISGDSGRLVRPLILVKDGKPQLTQIDMIDFKQNDAKTVFSNYVKNGKIEYLDVNESDNAFIALTINDVTIETTHLEIDQMTILSCVTGLVPFPHHNQSARNTFQCQMGKQSLGIIGMNTQIRSDTQLYQLIYPQTPLVRTVSMEAVNQHRLPAGHNAQVAVMSYSCYDIEDALIMNKSSLDRGFGRTAVYKKSVTECEINQRNKESRFNERIDEPPTKTHNTKKKFIKKYHALDSDGITKVGAQLHHGDIYVNKKTPQVPDNPQIENIQIADTPSVFKEKWPYTVDRVLIINNTNDGTEKIKTVKTIFRQIRRPEFGDKFSSRHGQKGVVGLIVNQEDMPFNERGWCPDLIMNPHGYPSRMTIAKLMELLCGKLGALEGKFKYGTX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XNB5</accession>
    <name>A0A8S1XNB5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DDH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1310110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000131">
        <property type="protein sequence ID" value="CAD8202581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XNB5"/>
    <dbReference type="OrthoDB" id="374045at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004309">
        <property type="term" value="F:exopolyphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12112">
        <property type="entry name" value="BNIP - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12112:SF39">
        <property type="entry name" value="EG:152A3.5 PROTEIN (FBGN0003116_PN PROTEIN)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDH" evidence="1">
        <location>
            <begin position="18"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8202581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="304" mass="36354" checksum="BF07671A86569118" modified="2022-10-12" version="1">MINQFLRNIKLLQNVKQLVLGNPTADMDSCVGSILLAYHMTQFNIPTAPIINYNRQSFGSHFETIELFNVDDLIFINDVDLNQYDLILYDHNDIKYTNNQIGCIDHHEDKGQQFSQFKKIEKVGSAVTLVAEYMQLDKNYKCKQEIVEISQLIMKTILIDTFNFQQNQYQIRWVDKDKQIFDLCHSFYPQFNAQIEYQHLTDLKYDVKLNLQLSLSQQLLKDYKKFYTVGYCVIFIKLQDLLQKYNEIQLINEFNEFMIREECKTLIVFFIHQENEYIQRSMMIYGENQLQIIKYRQCLQQENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XP56</accession>
    <name>A0A8S1XP56_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1300113</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000130">
        <property type="protein sequence ID" value="CAD8202242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XP56"/>
    <dbReference type="OrthoDB" id="301688at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8202242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="413" mass="49024" checksum="3B3457434BC98EBA" modified="2022-10-12" version="1">MSNCDSPLCLTPTDKRKLNVKLKVPDELLNYTPLEQLSIPKNPKIRSSFFNFLSGNINEKEFVKFKCFPSHQEQGVQVVTKIRNRHQDLTLQLKKQAAIDKARQRWHKLAEVIRQNIQLRKQIDTNYQSKIISRIELGLKIQNQQAHQSIIIDDLKNHQLRTMTIIKEKHQKQNQSCFISPKHYYYTLQGYLQNQSDEYLYIYHQNLPNTSKLTDKQYNQLLQTYLNKKQLFEKYQEEIKQRKIIKWKPLKSQSTFYNSSMSSKSFGLNINKPMQRMSNPLQSQQQQRQKEYNLINRINELANPKVANNVKTKKFFRQQQINSKLNGVVKHNRIFSFNQIDKEEDDVLDLSNASITEALLDQLYLGSQKLQTKLRQQPTVQKKVRDILRLQEMKANTINANYQKLNKYQQQHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XPL2</accession>
    <name>A0A8S1XPL2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T1330068</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000133">
        <property type="protein sequence ID" value="CAD8203179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XPL2"/>
    <dbReference type="OrthoDB" id="296179at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05580">
        <property type="entry name" value="STKc_PKA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000005">
        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF37">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT PRKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="16"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="10">
        <location>
            <begin position="271"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="298"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="298"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8203179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="323" mass="36569" checksum="8AF72906C341FD36" modified="2022-10-12" version="1">MSTGLTKPKKLKLTDYEVFQTLGTGSFGRVKLARNKQTNKYVALKSLKKAEIIRLKQVDHVINENTILGNLSHPFIVNFEGFCQDPRYLYLVLEFVSGGELFTYLRSIGRLDTSHAGFYASQVASIFEYLHSKNIIYRDLKPENLLIADDGYLKLTDFGFAKVVEGRTYTLCGTPEYLAPEILLNKGHGKAVDWWTLGILIYEMNAGIDPFSDEDPMAIYQKILKGKVKFPKSFDKNAKSLVKHLLVADLSKRYGNLKNGAADIKNHRWFGNLDWNLLTQKKLPVPYKPVVKAANDTSNFSSYPESDTQSPALKPADDPFLEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XPL3</accession>
    <name>A0A8S1XPL3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tubulin/FtsZ GTPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1320180</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000132">
        <property type="protein sequence ID" value="CAD8203030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XPL3"/>
    <dbReference type="OrthoDB" id="304008at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06059">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000044">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0493">Microtubule</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="6">
        <location>
            <begin position="15"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8203030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="402" mass="46240" checksum="04F5E3BBF3D692D3" modified="2022-10-12" version="1">MNYGNFIKKNKQTKMQKFIFTMKTIVIIFHQLYLWIQMIVWQMKSKRINQQITGKEDSANNYCRGHYTIGKEIMDKCLDYVRKQVESVEKIDQFIITSALSGGTGSGFSSLLLERLSVDYGVKVEKNAFLIYPSQEMSNNTVDAFNAVLATHMTIEHCDCVVMLDNQSMYKSIDSQIGLDYVDYTHLNNLVSQLISSYTGLRRFSKIDNKKLLSELCPYPRIHCVIPSYGPLASINDQINQELNEKQLISLITKPAQRLFQSEINPEHICASIVHRSKHNNQFFGQSDLTLQTMRIKHQNIPNVYQCLSQNYTSISELAQLKQTGILLSNDANLFNYFEKLGKKYDQIYSKRAFVHWFVGEGCESGEMSECREDLAALSKDYEDMTDGAIHSCEVFDEDDIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XSF6</accession>
    <name>A0A8S1XSF6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1340099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000134">
        <property type="protein sequence ID" value="CAD8203638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="321917at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035718719" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="16"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8203638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="529" mass="63641" checksum="CB87574B5F45C6AD" modified="2022-10-12" version="1">MIFFILIFFVQSCLWEYEDMTLYPTNGEYFEYPLSSLIQNYDDHKLIYHYEPSVPNVQITNIVNEITHIKGYEFISVTSNQTHFATITKENDVILYQWQNLNIQQYGQQVKIEERYQCYNIIFFADVDVLLDCYAEEYFYLLQLMNTSLNIVYSIQANKPIKSQMQGIYNESKTFLIYAQYYQNLSIITLFSSQFKNLTFYQDQFIQIGIPLRENPYIYILYKQYIKQFIITQNNTFQQTQIFTNNNEIEADNFQVYYNYQDYSQCDQILVNYHYRFQDNDKKQMMSSSKYLECQNNLYYQFELDNIDYKESKVIQSFLNNEFLLFQSSLRLIMYNLHGFHSIGNIQINNNTQIYLNTQDNYLFTFNTQIIVYELKIPQIQINLTDQQVQGITYDITIYAKLYNLTFAGICKIFMSITILDQSDTNIYVIFNKNFYQYRSITGNEINEQIFVGYSGKLLQYTVNIDNKQFGKDCYKQIKNLPWPNCLIVFAMIVKMIXYQNIQYLQKPLIYQSNFKQYFIYQIKQQLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XVM3</accession>
    <name>A0A8S1XVM3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Enkurin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1400019</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000140">
        <property type="protein sequence ID" value="CAD8205185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XVM3"/>
    <dbReference type="OrthoDB" id="308504at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027012">
        <property type="entry name" value="Enkurin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052102">
        <property type="entry name" value="Enkurin_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21490:SF0">
        <property type="entry name" value="ENKURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21490">
        <property type="entry name" value="ENKURIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13864">
        <property type="entry name" value="Enkurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51665">
        <property type="entry name" value="ENKURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enkurin" evidence="8">
        <location>
            <begin position="159"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="16"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="150"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8205185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="247" mass="29659" checksum="AE6D9006F28A1753" modified="2022-10-12" version="1">MSECIYKLQNEEQQLIEEQPKKTSQNQKRYKSQHPGNLPPSYSTFNTKNTTLNINNINGNKILHKGYLKHHALWGKPKNFEVTDELMFPLGLIKPDDCALKKPNIPLHFKQNVDMEKFKEYLENKPKRPKSGGQKPKQIDYFKKDTFGKVPEYLEKMKNDKLEKQRLEEEEIRRKKKEKEEQKIKKGDVFKIMQQLLQKKEELLKEFAQYSHKRDMTQQTKLRKEELEKRLNQMDGDIKKLERLYNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XVZ2</accession>
    <name>A0A8S1XVZ2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1380014</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000138">
        <property type="protein sequence ID" value="CAD8204632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XVZ2"/>
    <dbReference type="OrthoDB" id="306523at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8204632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="340" mass="39109" checksum="A9998FDA1D5278F4" modified="2022-10-12" version="1">MTEQYIFDSEIVEDEVDRPVSVYNLNVCFKEDIDFRLQDCQLNEPDIQDLEQKMNNLIYNSCGFKKGLIKHNKQQKQQSAIDLTENRTKNEQLSFKKPIQPIQQLSIVSNGDQSSLFSRKETVPKINFVGEMKDSVNQNTTVDFRKSLKLHTHGPFDITKLKDGLSPAVNNQFSSKKQSQFSQRSHETYNSYLELSYNQTQQIPKQHSINNLDKRKERSKIAKTTIKFISNQQNQKLRISQQANSQRTSQLSLLQLEQDGIRGRSQSSVSTPKSILKNPSLQNSQLEIVSRLSFQSRLSFSSLNITCQSPKKQQANSQSPAKKVKFCLTKKQARRENIPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XZJ2</accession>
    <name>A0A8S1XZJ2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1450077</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000145">
        <property type="protein sequence ID" value="CAD8206830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XZJ2"/>
    <dbReference type="OrthoDB" id="307976at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8206830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="961" mass="113929" checksum="5637C6B9FB617AE7" modified="2022-10-12" version="1">MMQEQEYDYTVQHTEAIQLYVHQQMKDPNEEYSRQCKKRKPNELMLYKDLSDRNPLLTIYRAVSYDEPDKQITINKIIQWLQNQQKTILQRSEKDKLSEKSFLKHILTQLCEKQQQESKPNPNPNPQEFIAMNQTFVHYINEAVEKQSEGYVNNILMMVQPNIKKFQNNNLNYFAQAVAHQMKQSKKAQIPTESPFRSNKVIFINLTGKESLQNLESWQSYIEKSDEFVYFSFIKVQGEEKNDIENQFNDYSNYFKQMEQANFNYYLLPYVCPQSQDQNNTNILHGQLVKAFFQNFMHNRIERFSHKGYIALSLSLSDDIEYDPFTIRFLINNLTKLSNDKLMIHINIEIPEEDSTYEKRLTQLMNLCYSSFSEQNISIQKLIPQDKIVLSELIDRLKKIYRYLNLENQYKSIQYCNIAIQNLKQEINRIKENPIQYPLANLYCIFYKRNSEQYLIIRPELENDDQITPFTQIIINYQDDIIILYQDGEKKFLYFLQFNNSYFNKKNLIEKIKFKNLTNFLESANIANQIVFYFNQQIYVFYGQQQNNFNLAGQRINILQMQSTPLTFTNQDKFQGNFHSKIAIQKLGNEQLIDKLKSRISPTVCIDNYKDQILKIVLFGGENIDTPQIMNLIEYMEIRDQQFTTGILDKSNLFKDISFKPFPGQTILSTNYQNDILYLILPGNDTLRKKSLNHPINTLFHKNALLMKKGCLDYSLSNIPINYYGDISYGLNFISNSQQNSQLIFLDDILAIWRVIYTQTMKIESIQKLKLDFSQAWGSKCANTEFSHKKEKLFKEQYHDIEMILAYLIEIQKEISEGDVIELIDKKNKFDKSKEKSELNIYNQDEQIKQKDYFKQKLDQGQLFDLSGNPFNQRDKMQEEKGNLNEQQQNPPEFFKSNLLDIYDGDNKQLYQIAYENYLYLDLQTKQLSVKQFTYLIGDALSLPQIDITNLLQVIKIQKMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y1I0</accession>
    <name>A0A8S1Y1I0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1480035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000148">
        <property type="protein sequence ID" value="CAD8207633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y1I0"/>
    <dbReference type="OrthoDB" id="298526at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="101"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8207633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="145" mass="17579" checksum="3FE9EF96EB23C3FD" modified="2022-10-12" version="1">MQQTHQLLDSIERKVQNCVIRDQQYEKLLFSGRPSHIENTIDKQQKQLGDSKITLLDHHYQKKFEKMGQRLENLEKEKEVKVQISREQQTSDFEQEEARSIVNLNRTIKLYSQQITSLQEQMKRMQMNYQKQIQDLQIQLRKYQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y4V8</accession>
    <name>A0A8S1Y4V8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1500032</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000150">
        <property type="protein sequence ID" value="CAD8208237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y4V8"/>
    <dbReference type="OrthoDB" id="290675at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802:SF155">
        <property type="entry name" value="DEUBIQUITINASE MYSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802">
        <property type="entry name" value="SWI/SNF COMPLEX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8208237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="111" mass="13053" checksum="D951794531AFFA08" modified="2022-10-12" version="1">MAQNDSEATKQSNELVSDSKKIMKKQGHWNEIEHKTYLKFLQENNNHTKGQRLFKKMSQIVGTRTPSQCRSHHQKFNPQKTKCLSETGILRSKQYARQYFAQHKTLDNDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y540</accession>
    <name>A0A8S1Y540_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1450109</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000145">
        <property type="protein sequence ID" value="CAD8206894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y540"/>
    <dbReference type="OrthoDB" id="306904at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="25"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="206"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="274"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="119"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="213"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8206894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="301" mass="34739" checksum="5EF4C0DB7DB34E63" modified="2022-10-12" version="1">MQITPKALTALQKLLSVNSPIYPNEIRNQEHSNSQSNQKSQQCQSSCQQNGIKNSLKQKFMSPQEGIESLLRHSIGKPSLNGKPQGNVKNELINVLKQNQNVKENTIKRNGNSLNQLLQPQINSPQKEQSAKNDYKKKRPPQLCDIVQDDDNTVQQKQIMTSKSLQKSPNNINKIGSGFFTSTHQKQTHMNVFGSLNEVLRQYIQQKSHKRQNGTDKNQEEKKVNENKFHSAQNKRISLSLQTNHSNLLQELYLKTSKILTQYQTKETLWKQQKNSLKEEVKFLKQLLQQQQEESNQKQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y5D8</accession>
    <name>A0A8S1Y5D8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1540004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000154">
        <property type="protein sequence ID" value="CAD8209235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y5D8"/>
    <dbReference type="OrthoDB" id="307365at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8209235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="298" mass="34996" checksum="13E65B3F0092E02D" modified="2022-10-12" version="1">MQQVKYPDLNMLSQETLSQQLFENNNQNIKFIPIVTPNQDQILQQIQAQQQQKSKDVSPYQEYSNYQVTQEQQSISNLIKDQDFQKQKKFLQKILGIFILWTIGACLTYIFFCYTLFRIKRIEVVFLLFILISLFSIPILIKVGIGKNYRNTSSSITILLGLILSYTIFYLGILGLIMFIIHEYWACKYRKLQKPWNQLLCQFYNANILKHGNTSNQNDLQQYIYRFQDVFFAQLVVIFLCGIFVKELSFALVLAIPYPFCVMNVFKQILKGRFNLQDDQVITGSITAFYGLFICCRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8E0</accession>
    <name>A0A8S1Y8E0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1530099</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000153">
        <property type="protein sequence ID" value="CAD8209167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y8E0"/>
    <dbReference type="OrthoDB" id="10306350at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8209167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="59" mass="7002" checksum="175D66344D596DA4" modified="2022-10-12" version="1">MSANVTITAFFGMADDDFKDDVYDRYIRDLHRPRKTIYVPKPPSIVTKHLNVRRINTEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y9L7</accession>
    <name>A0A8S1Y9L7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">LITAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1620083</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDIP1/LITAF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000162">
        <property type="protein sequence ID" value="CAD8211186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y9L7"/>
    <dbReference type="OrthoDB" id="5599753at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006629">
        <property type="entry name" value="LITAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037519">
        <property type="entry name" value="LITAF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292:SF6">
        <property type="entry name" value="FI16602P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292">
        <property type="entry name" value="LIPOPOLYSACCHARIDE-INDUCED TUMOR NECROSIS FACTOR-ALPHA FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10601">
        <property type="entry name" value="zf-LITAF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00714">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51837">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="LITAF" evidence="7">
        <location>
            <begin position="15"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8211186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="101" mass="11658" checksum="33C50372DA9AC51D" modified="2022-10-12" version="1">MSEHSHLMDENVVQSQINFKYKTKLNGISEPCEFYCFTCNQPRISRIEYSYSKATFIWCCILCFCTIILGLVPFCMQKCSSKTHYCPICDKIVGQTDGELC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YCS2</accession>
    <name>A0A8S1YCS2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1670062</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000167">
        <property type="protein sequence ID" value="CAD8212276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YCS2"/>
    <dbReference type="OrthoDB" id="7851174at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23792">
        <property type="entry name" value="UBCc_UBE2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000010">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2-16 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="7">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="5">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8212276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="148" mass="16737" checksum="412C949F6465F0C0" modified="2022-10-12" version="1">MALKRIQKELADLAKDPPANCSAGPVDDKDNFHWQATIMGPEGSPYQGGVFFLNIHFPTDYPFKPPKINFVTRIYHPNINQNGAICLDILKDQWSPALTISKVLLSISSLLTDPNPDDPLVPEIANIFKQDKNKYEQYAREFTKKHAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YDL1</accession>
    <name>A0A8S1YDL1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1550033</name>
        <name evidence="7" type="ORF">PPENT_87.1.T1660107</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the inner dynein arm light chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000155">
        <property type="protein sequence ID" value="CAD8209544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000166">
        <property type="protein sequence ID" value="CAD8212140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YDL1"/>
    <dbReference type="OrthoDB" id="296406at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019347">
        <property type="entry name" value="Axonemal_dynein_light_chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13183:SF0">
        <property type="entry name" value="AXONEMAL DYNEIN LIGHT INTERMEDIATE POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13183">
        <property type="entry name" value="AXONEMAL INNER ARM DYNEIN LIGHT CHAIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10211">
        <property type="entry name" value="Ax_dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0243">Dynein</keyword>
    <keyword evidence="3" id="KW-0505">Motor protein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="203"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8209544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8212140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="251" mass="30055" checksum="CD6774C870F02D79" modified="2022-10-12" version="1">MSQTEETLKKYPKYMPTLVRYEEPIEINEDEMQLEMQEQVLNQKKKQQLQPLDQKWSIDEILNKFFPPRRFEHEGHYFKQVVSVNDVKRDELNQLEAELDQRLIERQARKSGICPVREDLHSQLFEEIIRQSAINCPERGLLLMRVYDNLKLTFAAYQTLYAGSVVFGNRKAAESEIGKSEQDSKIADYDKKKIYLENQKILLENELDAIQRSFKEIRELEEKRMESEMQFLKQQTKHLEHFLKQVQQNQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YF03</accession>
    <name>A0A8S1YF03_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1680017</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000168">
        <property type="protein sequence ID" value="CAD8212400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YF03"/>
    <dbReference type="OrthoDB" id="300641at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052798">
        <property type="entry name" value="Giardia_VSA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23275">
        <property type="entry name" value="CABRIOLET.-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23275:SF100">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035900702" description="EGF-like domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="92"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="178"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="221"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="255"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="498"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="532"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="568"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8212400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="681" mass="73998" checksum="D72F52B1BD96A319" modified="2022-10-12" version="1">MNIKIVIISSLILTTFTQTTGQDCTQLSCIATGALKQEYTSRVPYKCMEGYYWGGQSCQPCKPIQGGHYACTSYWGTGTLYCLPGWIILNGVCVNMPNGCLTYQLNKDQNAYICGSCDSGFTLIAGVCVENQQCVTYSSTRYVCTQCQQGYYLNWNYIPNISSSNDNYYNYFGSFCNPCSIFGCANCPSDNTCTQCKPGFFWSFSGLTIDSTTSGSGTCQKCLDYCINCTNSSNCTQCSDGYYVNTINSITTCSLCSPTSTCVKCSNATTCTQCLNGYQLLNPTTCEKLYDGCIQMDSNKNCTTCQSNYILNAQTPKTCTQCGTGCLTCTLNNNIATCTTCQEFYYATKDSNGIITCVECKQYPQSIGWLRCGGPYDNPSYTTVQVTQCIDGYFLVNITSNNITTPTCIAAIPSSACLTLTTTSTNTNNFCATCIPNFNPYVDGTCLSCPPQQGAKTQLSNQCNKCGGTSTTNISCEGCNQMFFLQSANSANSATCATCSRGCLECQSNLNCTKCDIGYYKTGDSNNSTCQPCLKNCAVCTDSNKCTTCMDGYFAYSGSQTPQSSCVACQFGCATCNNPGNSCQTCIDGYVFQNGGCVPLNQANCALKLNSTTTPIQTYNSNGCSICKYGFHMISNRCYQSVQPYAGFAYMQLNEQTIINDNQKYEFVLKSIMIILLYLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YF08</accession>
    <name>A0A8S1YF08_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1630088</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000163">
        <property type="protein sequence ID" value="CAD8211447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YF08"/>
    <dbReference type="OrthoDB" id="296831at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8211447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="286" mass="32755" checksum="CC137C907466BF4C" modified="2022-10-12" version="1">MGCVSTKDQHIKQFNIKLPDSINQTTLQTDLDLSDSLDQYMQIGMNLSSIGELHPKVQLVHQELGDLFPNLQGQYLLMPDQSIYFGQTLNGKRNGQGKQHWQKEGNLLEGIWIDNQLNGRARMIYPNGDYFDGNFVNNVANGIGKFVNSRKEVRGFWLNNKLIGEGTEIRKNGTIYTGQFQDGKIHGQGKFEFANGCVYKGNVQKGKMHGNGELIFYDNTRYVGEFRNNCIQGRGIYEASIPIHGWFHSKYENQTIFLYFFRQETPVLVDNQDCTLIQKQLSTFFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YFA1</accession>
    <name>A0A8S1YFA1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tc1-like transposase DDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1590115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000159">
        <property type="protein sequence ID" value="CAD8210532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YFA1"/>
    <dbReference type="OrthoDB" id="2266637at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="1">
        <location>
            <begin position="249"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8210532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="366" mass="44119" checksum="79D781D2C49C02A5" modified="2022-10-12" version="1">MQIEYQREDLEDSINYNKYNFQQILEKDHICDEFCQDYKQLQRFSVRLYRIPLEIVTLEFQEAWDFCQSKIEVYFMKFQKKNNREIKRQKRQQKIVVKNIPFNFKIKSILHRIHENHISLVSQSVILGLSIERLKYLHKEIEINQNFHYIHPFEFVNDHSIWVSKYVRQENISNLFSIQNIYKDFLIQFNVTREELSISQFRKYSKQIGYSYKKTSKVSMQATTQINKEQRVQYALQFLFLIQNNLYPIYIDESGFGNDYLQKVWSQVGQKLHSNFITITKRINIIGDIGQGLFLFQCIRTNVDAHIMKNFLARLIQLCNEKFGRGLYFLILDNASIHKEQFLKQHIINKINHLQLPPQNNDCCQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YGC4</accession>
    <name>A0A8S1YGC4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1740016</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000174">
        <property type="protein sequence ID" value="CAD8213115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="778" mass="90490" checksum="940016DBF05FF6A8" modified="2022-10-12" version="1" fragment="single">MSSDQVVSRKLEQQLQEIEMQLDSSFDKIINFIDQVFIIDSPISKINNTKSDIKLPKVDYFHLMDQSEYISNIVEEVKSLIKYKIKDQVQIQSQQEQKNQQLLINSQQSQLLIQQQNEMKCLSQVNVKAFSYQIIEANSIKQQEYCWAVAINKDCSIVAAGCNKLIKIYEFQQGMLKLNQVLNQHKNNVFTLIFMKQQNQLISGDQRLILICNWIRCIILNNNEDLFISSSDDKTIKFWVKKNQWIFQQSITDHSSYVNQLSLNEQQNQVISCGYDGLILVIEQSETNKKWIVKQKIQVDSIGYRLCFINNNLFTFQPINGNLMYLYQINSILLQIKEMKVLNYFQNNLLNKNNQLLISIVNMYIQQDLQKMLNLKLSNLFNLEQIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQQQNEMKCLSQVNVKAFSYQIIEANSIKQQEYCWAVAINKDCSIVAAGCNKLIKIYEFQQGMLKLNQVLNQHKNNVFTLIFMKQQNQLISGDQRLILICNWIRCIILNNNEDLFISSSDDKTIKFWVKKNQWIFQQSITDHSSYVNQLSLNEQQNQVISCGYDGLILVIEQSETNKKWIVKQKIQVDSIGYRLCFINNNLFTFQPINGNLMYLYQINSVSKQFTKSKDITINQGNEGSELFPKQFIKQKQLVVNKHCEYVHLIRFTENAEFKVEQSIQFGTNX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YH04</accession>
    <name>A0A8S1YH04_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1700042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000170">
        <property type="protein sequence ID" value="CAD8212688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YH04"/>
    <dbReference type="OrthoDB" id="284468at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8212688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="162" mass="19160" checksum="1728F72A94842E61" modified="2022-10-12" version="1">MLIHNDSNFFIKPNAIVKLYKQLKPKTVETDRKRTNYILNKKTQQMNILHFSKPMLLLSIPEEQKSIRNHNQTCRSSTGCTRTPNRRRNLMTSPKESRLGYKILHYQEQEVPYEIHSQMPISQINPKFIIQQKQGCAINRLPNFKLQLFNQLPGLRQQLELC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YLM8</accession>
    <name>A0A8S1YLM8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T2420009</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000242">
        <property type="protein sequence ID" value="CAD8214730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YLM8"/>
    <dbReference type="OrthoDB" id="2654453at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8214730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="271" mass="31724" checksum="D442CFD143D6E7B2" modified="2022-10-12" version="1">MEKDDQVLGYNFGFNQEIRKFIRCKSKNLHLQQIQKRDLGKFPNYESLVIDTQCDYQRFITNQLQLKRIIAFIKQLQSNPKCRQLVRLIYKQFLTKQQLQEINQKLYFTQCQGHKSIQEFFVAKYIYNYILPYQTYPQSQNQMTVQELDNLDRLMNSVFNSHAFNINTDAFRRACSLITNILLNTENINQKLIEIVQQSIKIEFSPATLNSINLLTLMNVYLGSLNFNSIQLAGTNITGLSFFERNLSKSKLKNVAINSCIFNSANLIDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YLW2</accession>
    <name>A0A8S1YLW2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TNFR-Cys domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1680018</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000168">
        <property type="protein sequence ID" value="CAD8212402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="18487at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052798">
        <property type="entry name" value="Giardia_VSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001368">
        <property type="entry name" value="TNFR/NGFR_Cys_rich_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23275">
        <property type="entry name" value="CABRIOLET.-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23275:SF100">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50050">
        <property type="entry name" value="TNFR_NGFR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035772605" description="TNFR-Cys domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="2135"/>
        </location>
    </feature>
    <feature type="repeat" description="TNFR-Cys" evidence="1">
        <location>
            <begin position="836"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="3">
        <location>
            <begin position="836"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="837"/>
            <end position="852"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8212402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2135" mass="228561" checksum="B790EFE83F1969C9" modified="2022-10-12" version="1">MKVETFLYILLILGYFGVSGQTNQPGGVTTALQTDNQPLYLCDTGFFFNDQICMACLTVTGGKCTCKTYNGCDTLTCNDGYYQVGQSCITIPDSNCFRGGLAADGTSIVCYQCVANSGTTLIGGICVTNNNCLTFDLNTGVCTKCANGYYNVMAEFTPSEYFDTTESTWTSTNYPLYFQNINQCKTCNADASCKSCNFLKQCTECIDGYYLTGTTCTQCISGCQKCSDTTTCSVCLTTYNLTNSNTCLSCSTTQYFDTQNKTCGECNTINNCLTCSNSNTCTKCSDGNYAVNGSCVTCTGFPSTCSACDANSNCISCTSVTPMSILQGGTCRQCPSNCQSTSNCSYNTQNNTASCSLCQDLYYVTSQGQCAKCDSAGEPNALRCHYDANVSGNIVYTQCKTLYFLANNKCTQAQSNNTCAKLLVPTDQXKTYNGCDTLTCNDGYYQVGQSCITIPDSNCFRGGLAADGTSIVCYQCVANSGTTLIGGICVTNNNCLTFDLNTGVCTKCANGYYNVMAEFTPSEYFDTTESTWTSTNYPLYFQNINQCKTCNADASCKSCNFLKQCTECIDGYYLTGTTCTQCISGCQKCSDTTTCSVCLTTYNLTNSNTCLSCSTTQYFDTQNKTCGECNTINNCLTCSNSNTCTKCSDGNYAVNGSCVTCTGFPSTCSACDANSNCISCTSVTPMSILQGGTCRQCPSNCQSTSNCSYNTQNNTASCSLCQDLYYVTSQGQCAKCDSAGEPNALRCHYDANVSGNIVYTQCKTLYFLANNKCTQAQSNNTCAKLLVPTDQTAINNQSCSECWPGLIQSGSTCFSCDYCQSGSCTLNTNNQTSCTSCVDGYYSDSNQKCQSCISNCSSCSGPAIGDCTNCQAGYFKAASSCTACTVDSNNHTTCLVCQDATNCSSCINGYYNLNGQCLACQYGCSQCTSPGNVCSACINGFYLQNGGCVQDNGNCYSTDKLNILATVQQNQTNGCQICNYGFYLTSGWLTQQTSSQIQDFVCMQCVYPQNAFVCRTNPQDVSIQSVQTTTTALTPSSQYFSKGAIVTNISNFALLDTRYTSATCASGYFWTNQSCMPCMSITYGICTSCTNLTACAQITCNSGYFLTTINNSQLCAPMPQGCSELTFSTNLLALACTKCIAGYTIVGNICISNSGCQTINQSNGICTQCQEGYYFNWDFVLTTSAQTAPTTNTKYYTTFNQFCSQCQAGCQICPSQYTCTQCLPGYFWYQSTVTNNQQTFIKYSSSLTSNMSGQCVQCPKYCQTCTTATTCTTCYPNFQADPNSSVGACMCPSGYTLTTSGSTFSCIQQTTAIATCQITACSQCNADNTQCLTCLSSFQGKSYTLSGTTCVTCSSNCSQCTLMTGSTSQTTCQVCQNGYYKTQSANDQPVLCSICSQTGALLCQGSLENASFTQFVCRTNPQDVSIQSVQTTTTALTPSSQYFSKGAIVTNISNFALLDTRYTSATCASGYFWTNQSCMPCMSITYGICTSCTNLTACAQITCNSGYFLTTINNSQLCAPMPQGCSELTFSTNLLALACTKCIAGYTIVGNICISNSGCQTINQSNGICTQCQEGYYFNWDFVLTTSAQTAPTTNTKYYTTFNQFCSQCQAGCQICPSQYTCTQCLPGYFWYQSTVTNNQQTFIKYSSSLTSNMSGQCVQCPKYCQTCTTATTCTTCYPNFQADPNSSVGACMCPSGYTLTTSGSTFSCIQQTTVIATCQITACSQCNADNTQCLTCLSSFQGKSYTLSGTTCVTCSSNCSQCTLMTGSTSQTTCQVCQNGYYKTQSANDQPVLCSICSQTGALLCQGSLENASFTQVTIIQCQNNYYLYNGACVAVPTTATCYTISSSNGSTCQQCLPGYTLFGNTCQTCATSKNQNCLTCTANGDNSGLQCTSCQSGYVIDSTNNVCVACSSGCTKCTLIAATQNTQQSTSCSNCNDGNYLDNITGRCFACPYDCKTCDSRYTCSTCKDGYYLKQSQTTLNAKQYTFKPCYPCQSNCATCTGPNGNVCLSCSSGYVMQNGGCVNLQQTIVNDLNKYNYANCYQLDPNNSGCLNCQAGFYLDAQKVCQECVSPYNNFVCGLKAVSPTNPVGPGPNPNPGPNPTPTDTTVQIGQNPVPPSSYSIILGMLIIIVLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YPG3</accession>
    <name>A0A8S1YPG3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T2350001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000235">
        <property type="protein sequence ID" value="CAD8214657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="66906at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015374">
        <property type="entry name" value="ChAPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF8">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09295">
        <property type="entry name" value="ChAPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="38"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="170"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="238"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="340"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="510"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="796"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="1186"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1757"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8214657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1757" mass="205130" checksum="704DF21B25981060" modified="2022-10-12" version="1" fragment="single">MIQNNEDFIQAKQKATSQDIQSQIEACYLELQQNIDISFNKIDNFLDSIIDDTLNTFNQNFLISEQSTTIQYNISQPSFQIPDFANQMVHQIVPLVKALIENQIKKKKEFLTQDFIQQEQLNPNFEQDLFQNQQLEIQDQDILELKQTQTLVQQSIELDDNLQQVDPNNLEFMYQQANTLTILNKFEDAKQLYKDIIKISPNHELSYQKIADILRDQKYYQKAKDFYSQCISINYYNSDAYFGIGECLKETQQFYEAQYYYVKAIEINQSNLQYKFCYGNCLRTQGKYHEAICVYDECLMIDINHAETIAAKGICLIYIGMEDKAFNYIDYSINLKKNSRLAIFGLGLILLTKKDYLKALEKFDKALEIDPQSNEILQIKIDVLIQQGQYQQVIECFDKIITLNPQSDVTLKNKIYDLTNQQKLEEAIQLFDKSLKINPQSIEILEIIIDVLIKCGQQEQAIQLCDKSLEIKPQSIEILKMKIDVLIKCGQQEQAIQLCDKSLEIKPQSIEILKMKTDVLIQQGQYQQVIECFDKIITLNPQSDVTLKNKIQDLSNNHKYEYAIYFCDILLEINPQSIEILKMKTDVLIKCGQQEQAIQLCDKLLEINPQSIEILQMKSKVNLNLIVDVLIQQGQYQQVIECFDKIITLNPQSDVTLKNKIYDLSNNHKYEQAINLCDKLLEINPQSIEIQQMINTINRKILVNTLEQSGYPYYGFSCFEKINPINVKFGEQFQLKYLSNIHQFEKAINLCDKLLEINPQSIEIIQMKIDVLIKCGQQEQAIQLCDKSLEINPQSIEIIQMKIDVLIQQGQYQQVIECFDKIITLNPQSDVTLKNKIYDLSNNHKYEQAIQLCDKSLEINPQSIEIQQMKNTINRKILVNTLKLYEPLYKDFYCFEEIYLTNAQFGEQFQQIIQDLSNNHKYEQAIQFWDKLIEITPYYIAFLQMIIDVLIQCGQQVQAIQLCDKSLEIKPQSIEILKMKIDVLIKCGQQEQAIQFCDKLLVINPQSNEILKMKIDVLIQQGQQEQAIQLCDKSLEINPQSIEIIQMKIDVLIKCGQQEQAIQFCDKLLVINPQSNEILKMKIDVLIKCGQQEQAIQFCDKSLEINPQSIEIQQMKNTINRKILVNTLKLYEPLYKDFYCFEEKNMTNAQFEEQFQQIIQVLSNYHKYEYAIQLCDKLLEINPQSIEIIQMKIDVLIQYGQYELAIECFDKIITINPQSDVTLKNKIYDLSNNGQYEQAIQLCDKSLEINPQSIEIQQMKNTINRKILVNTLKQNSYLYQDFYCFEEINLINAQFEEQFQQIIYDLSNNHKYEQAIQLWDKLIEITPYYIAFLQMIIDVLIQCGQQVQAIQLCDKSLEIKPQSIEILKMKIDVLIKCGQQEQAIQFCDKLLVINPQSNEILKMKIDVLIKCGQQEQAIQLCDKSLEINPQSIEIQQMKNTINRNNIVNTLIQCGYLSQDFDCFEQINFIFAQFEEQYFQQIIYDLSNNGQYEQAIELCNKLLEINPQSIETISMKINVLIQYGLYEKVVECFEKIITINPQSSDTLKTIFGCLIYHGIYQQVIQKCDKIMEINPQSDEILKMKIDYLKHQNQFEKAIQLCDKSLEINPQSNEIIQMKSKISLNLIVDILLQFGQQEQAIQFCDKSLEINPQSIDILQIKIDFLLQFGQQEQAIQFCDKLLEINPQSIDILQIKIDVLIKCGQYQQVIECFDIIININPESDVTLKNKIYGLINLNQFEQAIQLCDKSLEINPQSIXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RVN3</accession>
    <name>A0A8S1RVN3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0010415</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RVN3"/>
    <dbReference type="OrthoDB" id="289219at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035857695" evidence="2">
        <location>
            <begin position="16"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="87"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8132046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="381" mass="44738" checksum="582215B138270238" modified="2022-10-12" version="1">MKLILLITFFTICICLRYDDGNSGNSVIEEQIKEIQQHKIGQAILKMAELNYKLQGPVDELISTIKSFGRQLIERVEQENLDYEAIKAESVVKVQQLKQIISDAENEQNKQRKRIGQDLELRKNDIEKELLRLKDSKQQNESRLQMQEALRKQQYENFEKSKRDIGDLIKITDDTLKMVGSLLPRPDCSFIEMDKIQLSKEESKDLTRRLRNIKGRVDGLKGYENVMETLVQLTDSNFKQRHFIKSVLDLLNKLRKSLVESQNKLINDEKEQERAFSQWDSAQDNETNVFDREWNDLMEERDDIQSFIADCENIIKIHQADIDLYGERVKLEEQSLLISKQTHEELVKQINNELDIIQKSIKLLYSPAIFEYLKYKINLGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S2Q5</accession>
    <name>A0A8S1S2Q5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0020286</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S2Q5"/>
    <dbReference type="OrthoDB" id="71437at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051980">
        <property type="entry name" value="WD_repeat_MORG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22842:SF3">
        <property type="entry name" value="WD REPEAT DOMAIN-CONTAINING PROTEIN 83"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22842">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="18"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="61"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="103"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="273"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8133204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="308" mass="34860" checksum="08310D72921DB516" modified="2022-10-12" version="1">MNKSKTQNFHLYEPQRALQGHKAQIYVAKFNFDGQYLLTGSQDRSIILWNPYKETQLKQYFGAHNYEVQDLAILQDNSKFASCGGDKIAFLWDVSTGQVIKKFGPHSTRINCVGIGGEQSILATGGYDCEVKIWDLKDKKQNQPIQRIKEFQDSVTQISFQNHNMIISSIDGHIRTFDLRKGIIQEDFIKAGIFKFSSSFDDRLLALSCNDSIIRILEKSSGSILAALQGSHISNQYSINCEFSWNDGMLLTGSEDGKLLIYDILTGKCQITRKLHDKCLSSITLSKQQNAFVTASYDNVALLWMEQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S9A0</accession>
    <name>A0A8S1S9A0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0050265</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNase II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S9A0"/>
    <dbReference type="OrthoDB" id="284919at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004531">
        <property type="term" value="F:deoxyribonuclease II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09120">
        <property type="entry name" value="PLDc_DNaseII_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09121">
        <property type="entry name" value="PLDc_DNaseII_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004947">
        <property type="entry name" value="DNase_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10858">
        <property type="entry name" value="DEOXYRIBONUCLEASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10858:SF23">
        <property type="entry name" value="DEOXYRIBONUCLEASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03265">
        <property type="entry name" value="DNase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035871540" evidence="3">
        <location>
            <begin position="16"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8136668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="339" mass="38668" checksum="641A7DCD24174544" modified="2022-10-12" version="1">MLIFLFLTIATASNCLNQYGQIVDYWLIFKLPKDTVNQYTGMEYYYCDSTTQCAELKYQYDKLNDFTSALQVTMEQVHFKDTNTMNVIWNDQPFDKDYYPDMAHSKGVLSASLNGQGFIINHSTPKFPIMDSNYDEIILGMPSNSYTNAQHFMCFSVTTSEIERIAQQLIIAEVITVRANSPSDFKTKYPNLYSLKDTTRKSQQSSGSLTVQTRQGLSLQVISTNQHNQVDFYASIVAPKLKVGLVVQTWGSGGLQPPDCTSSYQTFSNLSRLQNGYKFSYTKDHSKFGISLNSNIPYVCMSDINRQVSQNKRGGTAICFIHHNIWSQINYQFIERQQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SEC0</accession>
    <name>A0A8S1SEC0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0070009</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SEC0"/>
    <dbReference type="OrthoDB" id="307433at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="35"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8138226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="163" mass="19048" checksum="EE6F88CD7418DC96" modified="2022-10-12" version="1">MNFSIHQLQQLFEDKEKSEPHTPIFNGKTGSLDKLDFLISQIEEIRLENIQFQNQIQIYQNELNSKLKNNDVINTQIKEIEKQIQNEIIQNKLLTFNYIQKHEDCFEIKKQIINNNRQDQIVSIPDLNNPNRPSDCSTAPSPGMKVQIMRATKKVSSVIFSDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SEV0</accession>
    <name>A0A8S1SEV0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0050464</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8137024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="8061355at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03263">
        <property type="entry name" value="ABC_subfamily_A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000335">
        <property type="entry name" value="ATP binding cassette subfamily A member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000298">
        <property type="entry name" value="ATP-binding cassette sub-family A member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF36">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY A MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="93"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="688"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="728"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="772"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="809"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="845"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="878"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="923"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="315"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="984"/>
            <end position="1216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8137024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1299" mass="148813" checksum="CC3F8760C05E2ACF" modified="2022-10-12" version="1">MPSIILQIRALLVKNFTQSFRNKEYISDLALPVVTAIIVSLKDQLEFLGFFAPLFLSIAVSTPPRSLLINLVEEKSERFKESQKIMGMKQRSYLIGWIMYGYIKTLMSTIFLIFFWWLLTLIINDGDYGFKTESYKIFLTYFLYSSTVLNFSLAMSTVFSSPKLANEVSTFVTILSILLTFLVFLSSIQSSSLFYYCMSIFPQSSISFIYMGALKKGFFNSQAISTAYPLSNAALQLGIESILYFLLFFYLDQVFPNEYGVAKHPLFFLQGLFGKSKKNKLNDSLLYDDNSHDHNEDYSSAIYHQKLNIQKKCTVSIKNLTRKFGEFKAVDNLTIKLYEQEILCLLGHNGAGKTTTISMLTGMIEKTQGTIQINGIDLEENIDAIRENVGICTQRDVLYISLKVIEMLIFMGRVKGLEGLDLQQEINEIIEVTELEEDRNKLIKELSGGSKRKLSLAIALIGGSSVIFLDEPTSGMDAQSRRIIWEILQKVRQQNRTMILTTHHLDEAEVLADRITIMAAGKLLACGKCDYIKTNFGEGYHLSITSQNQNILQSCSDQVLSIIQEAQLDPQSQSDTKIFLIPFKSKQKLDQLIETITKQYKNDIVINLKLNSLEDAFINIGMDEEKFIKKAKGSKASVNDSDIAMEQEVEQQRDIKIPKCLNDPPVYSFKSQFTAIFLRKFYTTSRTFSNYISIVIPFTLIVIGTLIIDEINLDFLKDNKNLEQYFKLVLISLFVILAFCFNSSLFITLPVLEREFNLKYALTVMGCRVLPYWIGTYAFDFLLYSFFVLTFVIFSYIMQLSFVTDHMGYVVFAFVTFGFAYISFSYFAGILMYKKTSTAMKTFPFLNFFIIYCMPQNFWGVCALLWQKGIGNADFIEFLIKAIECIFSFLSPFFAFQRAFQNIIKIDVSPNNNQPPPLTTETLYYSIAMLYQGIIFFFLTLYLEGKQFKKVGNNLDNNDVKRSVRVDQQVIEQEQNLLKSNDPVKMYRLQKVYDNGCVALQNVSVSIQKQKILGLLGPNGSGKSTIFNILTSLINKSGGYVKIKNQEVHRGKHEIFQDVGICPQFDCIWENLTPKEHLYLFGRMKGLSGDDLTESVTYFLQTMQLEEYINRQSGRLSGGNKRKLCVSNALIGGPCIQFFDEPSTGVDPIARRFLWRTLKLGVQLRQSSVMLTTHTMDEAENLCDKIAILVKGQVYCLGSPQELRIKYGDGYDIQLREYKNKQEITQFLNKQFQNIIEIPQKDQENLQFHIPSQSFDFHKAYFSLTELVQKKLIHDFSINQSSLESVFLQFSKSQQDQYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SHJ0</accession>
    <name>A0A8S1SHJ0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Anaphase-promoting complex subunit 4-like WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0070474</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8139106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SHJ0"/>
    <dbReference type="OrthoDB" id="439331at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024977">
        <property type="entry name" value="Apc4-like_WD40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47822">
        <property type="entry name" value="CARBOHYDRATE BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47822:SF2">
        <property type="entry name" value="F-BOX AND WD-40 DOMAIN PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12894">
        <property type="entry name" value="ANAPC4_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Anaphase-promoting complex subunit 4-like WD40" evidence="3">
        <location>
            <begin position="56"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="195"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8139106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="376" mass="43559" checksum="47556F4256931728" modified="2022-10-12" version="1">MRPKPRANTKEQQNAEEQQPQHVIKEEKPISDKYQVKYNSKTQLRLDEKESFVLGVPGTKIYSVKWDADDKYIACACENGTVRIFNVRKRQLSYLINSLMPNIPFSYVKWRPQAQQFKTRNIFVTGNTKGEVQHWHMTSGKCLGTMKEDNSDIYCIDYNQDATKLAVCGLNPIIRIHDEEKRVVDVRLGVEQTQPPGHNNRVYCVKFHPQNPNMLISGGWDYRVLIWDIRQKKPEANQIYGPLICGDGIDMRESFDGQQLLTASWSQEKQLQTWDLRTCKLICNFDWNSQIKVSNQPCQLYSGQFSRQFDNQLTLAGGSGENEVRIFDSQDFDNAQICIHDLCREVNTVDWAHKDSRFAFSGGDGYLRIFEIQQIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJ67</accession>
    <name>A0A8S1SJ67_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0080088</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJ67"/>
    <dbReference type="OrthoDB" id="301808at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8139417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="598" mass="71469" checksum="8DC27EF31879A358" modified="2022-10-12" version="1">MGNNQSQKNQTKSKKKDPLLFLFGNNKKQIIFSKLSLKTLSLLASFLTIQEYHNILLTSKATYQLFIQTSGCFQIECHRLLNVKGDLIISQNWKKIIQSIVFIPIRGIPFSNAIKSVRQQLKQIPIIIEQKVNYSGFQKAILYFQNKSNQQLFPFNEYQESKQFANKNQELVLKLRQGLEVYIQLPQLLMQVYNLHDYIKIYSLYLEIKFQKCNLQQFIELWHHYTGWISILEQETKGIIAQFNNIIDTTFPQYNLPNYTVRQFMVSQWLKKSDRGEIIVMLREEFRLKMIESRNKNIKFQELRDYVQYLIDISTNQTNIHQYGYYNFEYSEELNKLIHLAVDLSPLLTVDYKTDENMLIYIFGQYIYDYQLFELLSTFRIEQFKQQIREHQQSSQKMSKIVVKQFEDQPSLSNNNLFDNQIQGFNYHLSMQYNIQFKIRQLFKQLSYLDDDICSTLENIQVESLEASQTSFASTNCSSFFSSIKNNDQLYHYVQFYQQELFNKLELFYRRELTINQYKQDVEQKNEQIFDIPELGQYHELQFAQILELFNNKQQRLNAILQSEKQLTMSIFNQQIIQQQICTKELSQAEQLLINILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJB7</accession>
    <name>A0A8S1SJB7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T0080361</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJB7"/>
    <dbReference type="OrthoDB" id="283727at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000210">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000048">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010926">
        <property type="entry name" value="Myosin_TH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06017">
        <property type="entry name" value="Myosin_TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51757">
        <property type="entry name" value="TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TH1" evidence="13">
        <location>
            <begin position="1"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="211"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="12">
        <location>
            <begin position="469"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8139920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="538" mass="62895" checksum="C49CB2FBFC4BB98F" modified="2022-10-12" version="1">MNSSSIQNVNGLDRLQMCNSKDLQNQFEEGEKVILSSNIIKVNQNNKKQERIFLLTNRFVYNIKSDSKILQFFGKSIKIQRKIRIQLISAISVSNIGTEFVLHIPQEYDYRFQSSDFRKTILETLIIVYSSLTISKMPFFFKDDFTLIQYCTTEKDVKKGIKRYPIEKPIELDVVEFQDYQKNKPLPQQLIYKKPGLQKQYSTSLISIEQFDLIKVLGRGAFGKVMMCEKKDTKELFAIKSLRKEHILDKNQVEHTKAERKLLEEIDHPFLISLEYAFQTQEKLFFVMKFMRGGELFKHLRDKKRFPEATAQFYAASILLGIEHLHSISVIYRDLKPENILMDEFGYIKMSDYGLAKFLKQGEKTYSFVGTPEYLAPEIIKQNGHSFEVDWWSFGILIYEMLVGRPPFFSQSQSQLFRAIIESDIVFPQQLSLSNHAKDLITKLLKKNPFERLGHNGGGVSIKMHPWFKDYKFQDLLEKKIQPPIIPKLYDKLDVQNFDLEFTREEAMNSVVNMDPKLVEKFKEDFGGVTYIPNNGLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJW6</accession>
    <name>A0A8S1SJW6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Autophagy-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0080169</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATG8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJW6"/>
    <dbReference type="OrthoDB" id="285525at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004241">
        <property type="entry name" value="Atg8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10969">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEINS 1A/1B LIGHT CHAIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02991">
        <property type="entry name" value="ATG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0072">Autophagy</keyword>
    <keyword evidence="1" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="lipid moiety-binding region" description="Phosphatidylserine amidated glycine; alternate" evidence="1">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR604241-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8139579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="121" mass="14379" checksum="9EFB417C0944C5CF" modified="2022-10-12" version="1">MKQTLNFQNTRSFDERKNESDNCKQLYPNDVLVVVEQSSKSKLPKQNFIKFKMPNSMTIMNVLQYIKSKIKLSQYDSINLYCGKTLLRIDQNLKELHEQFKDLDGFLYINYIEMNSFGTQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SLD7</accession>
    <name>A0A8S1SLD7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0100140</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SLD7"/>
    <dbReference type="OrthoDB" id="765884at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052758">
        <property type="entry name" value="SRC_co-chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44200">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44200:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="16"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="243"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="281"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="370"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8141485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="541" mass="62090" checksum="83952A8529F8F33B" modified="2022-10-12" version="1">MEVERNTGNDKLRQQAEEHKNKGNDYFKRGLYSNAAEEYEKAIELFANEPNYYGNRAACFLQMKKYNKCLKDCEKALSLDLNNIKFLRRKALSLQYLGLFNEAKPIFEQIVNLDNSEQSLKEHKQIKELINYLQQAKQKLDSNQYKEALQFIEKVAKEVPDAVDIQILNCECLARTSNINQAQEQLRLIQDKHGPRAETYYLKGLIELYGGSPDKAKSILQEGVRQDQNNKKCLAAYQMAKDQDSYKSKGNDCLNSNKFNDAIDYYTKALEVDSNNFKFNSIIYANRGLAYQKLKDHRKAVNDFDKSIELNEKYFKPYLRRGDSRQELGDLDGAQSDYQKVMELDQGSIQQMRQKINDITRKQKQLSKKDYYKILEVDKNATDADIKKAYRKLALQWHPDKNKENEEQKKLADKKFREIAEAYSVLSDKQKRQQFDMGVDPNDPMGGAGGFETNIDPTQIFKMFFGGEGGADFGFGNMGGGEFPGGFHTMFTSNLGGMGQNMRGGSGFPFQFGDFSQQGGAGFPGFQFQGMQFTQQQQRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SPI5</accession>
    <name>A0A8S1SPI5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPENT_87.1.T0090042</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SPI5"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000315">
        <property type="entry name" value="Calcium-dependent protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="119"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="413"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="413"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8140374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="453" mass="52846" checksum="C9E700DB9D8C8042" modified="2022-10-12" version="1">MFKESIFQGNQTKLIWSYLPMPVLSKKGALDGEFIKSSTNTCKIKKFILIQNILGCYTKKGPIKWINYENSFIEITRDKMLGEGISITKGETLILYGETEIWFNYMKRWCIQQGFSNTYKIMEKIGRGNQAEVYSCRYQLGDEKFAVKQYSKHKLTNRQDKIALLKEISILRQVQHENIIRLYEVFENEDTIYVVMELLEGGDLNDYLKQPSSFFNEKQLASFIYRLFKAILSFHKFGIMHRDIKPKNIILRKQSLSQFCVADFGLADYYNKDGKYLFQRCGTPGFVAPEILRDEFYDFKVDVFSVGVIMYIIISGEEPFGGDYNETITKNYLGQVDVSNLKISEQGQLFLKSLFEVRQLRPTVKEAMNHQWFKQEKIVTEKYRIRIKEPQIKQVQLFIPSITETTISHSQFLKPTRTASSTRNSNYRLSPIALKKPNKTPNPSPSLKRKSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SSQ1</accession>
    <name>A0A8S1SSQ1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EML-like second beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0110032</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="219"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="267"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="405"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="453"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="495"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="537"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="579"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="621"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="663"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1090"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1132"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1174"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="4">
        <location>
            <begin position="1182"/>
            <end position="1349"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1216"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1258"/>
            <end position="1299"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1300"/>
            <end position="1341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8142227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1421" mass="157075" checksum="80F9E23AD0D4BFB2" modified="2022-10-12" version="1">MQGYFQEKDALVDIFSKVKDVDEQIFGLILKILRKEKAEDCIGFLSDIGNQRKLESQILQQVENFTQADTEQKFSVVRKDLKQTNYVIKELKDHNFNYKDFSSKENKKSKLNLIQSIKDNKQIIEFLQFLVQLTSIDVTLIQCGSNSLHILVQMKVDLRNKNFENIKIQNTSLVGANFVRCNFSGSQFNNVNINGINLNGAILFNCKWRDLRIPELIQLHGHREYVRSVCFSPDGKTLASGGGSQYGVYDFSIRLWDVKTGQQKAKLDGHTSYVVSVCFSPDGNIIASGSYDGSVLLWNAKTRRKRAKLDALTDTVNSVCFSPDGKTLASGGGSQYGDCDFSIRLWDVKTGQQKTQVESDTSCVMSVCFSPDGKTLASGGGSQYGDCDFSIRLWDVKTGKQKAKLDGHTETIYSVCFSPDGKTLASGGGRSQGIGDFSIRIWDVKTGQQKAELDGHTSDVQSVCFSPDGNTLASGSNDKFICLWDVETGQQKAKFDGHTHHVYQVCISPDGNILASGSADQSIRLWDVKTGQQKTKLNGHTSFVMSVYFSPDGNTLASGSADQSICLWDVKTGQQKVKLDGHTHHVYQVCISPDGNILASGSADQSIRLWDVKTGQQKVKLDGHTSHVVSVCFSPDGNQLASGSYDKSILIWDVKTGQQTAKLDGHADYVNSVCFSPEGNTLASGSYDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSIRLWDVKTGQQKVKLDGHTSHVVSVCFSPDGNQLASGSYDKSILIWDVKTGQQTAKLDGHADYVNSVCFSPEGNTLASGSYDNSIRLWDVKTGQQKAKFDGHTHHVYSVCFSPDGNQLASGSYDNTIRIWDVKTGQTTAILDGHTSFIHSVCFSPDGNVLASGSYDESIRLWDVKTGQQKAKLEDHSNYVISVCFSPDGNILASGCYDNSIRIWDVKTGQQKAKLDCHNNGIQSVCYSPDGNTLAYGNDDNSIHLWDVKTGQQIESSDKNYKDILTQFKIPLQQNSPILEVPNYITTLLISQKAIFKSKGALILKGEFFNQSGIDIKSLFKKKGSCIIEGFQQQRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SW24</accession>
    <name>A0A8S1SW24_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0130164</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SW24"/>
    <dbReference type="OrthoDB" id="286314at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8144310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="210" mass="25031" checksum="1AE194E53357F16B" modified="2022-10-12" version="1">MNTSTSITNKNQSFKRVKERLVCEAIEKVERWRQMFTEGLQINNGEIIKVTLNQAAEIVGIPKKTLEDYTQLFKKVRLLTADIQQFSNEKMGFLRSYLRKNQNKLKKKLKDIRLKELEAQRKTQEIILEQNLLLKQKLQEDPETTESCGSQQESSNLMMCDDQNYIDTEIQFIQEEDLNFQDYESHQYYFNRVSNFKQEEDIDNYFQSTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-06-18" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SX06</accession>
    <name>A0A8S1SX06_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0120389</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="295619at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="784"/>
            <end position="814"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8143757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2029" mass="240938" checksum="410698EE76122D21" modified="2022-10-12" version="1">MIQNWIGKLLFNICGDLVEDFPNNEIEINKWQGVGEKRNIKFKQNKVREWMMEYFGLNVDVKRAEIELLQFKIPWNRIWKEPTKILVKDAFICVQTREEFSVELMRLQYQSDLKDFIEHCYKKLFNNFTNTKKQSKLKRVLKNFFSEMIILEVQNFHLRLEDENNAVKHNRVALGVLFKTLTLQPSYNVVSVKSIQSQKNEQEIKRKLDFSVEALSMYLKIEPTSKQSNNLSPDYVEQQNVIIDPLSFRILIHTNLTQQEEIPLKYIEIQFGDDIKINIDNQQFQTILKLLKLRDWYCQQGDHLQYRPQYKSSPKDNPFEWLLYWKQIVTIIRCRINKQKINLKSKMRNDLVKYIIVDFLHQQYQQNDDNKYFIDEQMILYTDIMQNKQQIEDKLLQEEIEFVFYKVNKILNSPEQLKEILVKMNLEQPQQQEKKQTIWSYFWKQKQQPEEGTEFQSKYEKLYTQISQNENRCLKRLEIQISNILINLKIAQERSTVFMKIELKESLLQYESFLKIRKFILKLKRITAYDCLNPNKDFQTIFSYHSYIERSQRNQYLSQEQSQIEISFEYNKEVNKSLFKVKSLPHLMSLNDTVLARLQPFLKQLRKNDYFYDIVFISQGNFKKSEFINVISNLCKANSLIIDINISHVTIRIPESAIKTNCPVLEITLENYLLQFLNTVTQQINKFAINFQYVEKNCKQSIHILQPVSITTKVVQYQNQDEQNKLTELTDLVLENKISDIFITIPILKTDFFKNFVYNVLGTLDNLKKISIFLQKEDSVNQSSSVNSVNRRKRSRSWSQQRDQSSRREDCSSQTSEIYYDAEEFINIPFIQSITQSRQQVSPQEKREEMLKKWNKIRINLNYLVNKIFKEADPKISQFLESLRLKAVFELQKFQIKVAIQKNNDRNSYFYIDNINVTIEKAFKVKHFGFQLQINTITLSIYKLHQLKQLKKYSGPQSDDIMAHINEDMLNLLPFLLRIKELQIYYLISVKEIKLELQNIKDTNSNYLTTCLSNFNLQSQLPFKIMYKEKLSDKPFFLEINNSVSSWDTTCDCKFILNILYYNGKLKLYEPILDTCEAIITFQKSSEELCFETKFNQLLFNITPQLIFTLYSLNQHSLQIKQFLLQDLNASQDQIVVQKQTLTQKFEIEIEHDNAKLLVDQIEIQHSQHYKTKYRIQLQTASSDDEISNEKTNQNSRHVTFHDQDPSPLMKQQTPNFKKQTSLLEEEILLDQSNEKDTIKRIASEIDVSEKQRFVTEILGDYLPIEQSTKYIFVNLTVQKLLFNLNGIEQYINEKESKFVRCTEVMAFKITSQRNFYRYSSLIKTKEERTYFLKTDNRDHKLKTIYYLLSIINRNQNQIIIYQIEQLPKAPYLIKNKCSQLIIFYQQNFEKQQCVLEPGKSSGFCWEDPFQAPQLQLEIHYEGQTKRFLIDIDRFNNQELQIDKQLDSTIDKEYSLEIFNDGLQKVIVLAEAQSLKKKKKSYKITCDLIGFSFIDEWEILYAEINNTYYIQQQQQTDIVTYQISAENIQLDACGLPIPKRQLYIVNNQINNFNKSVGQKKSITQSQQIQDTTSGLREQLKLKIKQKTISEILVHLKYVKIRIPNYKIIIDSNLLLQVFSFIKHTKLLLDAFNTPKQKSKSLLLQQSFEKLNNSNISDKQIQIQPKKIHIDKVIIRPFSVKMRFKNLLNMEQELKTLHHQAGLITFVLNQFQQLNLEFQQYIKTDYQQFMDANYELHIKALFNVFGESINQQIRCQVNPTNFLKNQGNLAKQFLTELKNLQSEMDKNDIFQGGLPVKFQLLQTFCVISKSLYDAAQETFVSVLLGIGKYCETSQPDQTIQRTSFSNKLSKLFRKVSDFQFKSQNTLEISFQMLISKLKTRRYPKYIHKNRQITSYNEDLSKFQLYRLWKLGLDQQFDHFQLSLNCYLFLSNQLFILLDTQYNQILLEMNQRMFQSITNTNSTCVIKYQKSNVNKQEGDDDIGMKQLKVEKLMLNFTIDEKILQSITHIIKSNLEILNVKENKIFSIMMLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T0W3</accession>
    <name>A0A8S1T0W3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0140113</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T0W3"/>
    <dbReference type="OrthoDB" id="305764at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8145179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="311" mass="36740" checksum="8C8032BFE8C392F5" modified="2022-10-12" version="1">MATNTNGIVTCCQDFGVYCITLCVSEVIALGVLAVGICQLIRRKKQFRQAKKRKNIIYILIILCLICNILFNVAQKTLLVFYFFQLYVYYAFLTYIVFYFARKTVKIKTFQPHKMLQLKIYCICLISLFTIFLIWNLYDYVSNLSKSYWLCQSICNQINLRIDISYYKNDWFYSGFSFNSCCSFFKYEYQKESSIKINNVKVLVCVTFLGQLLYFIEDIYFLAQGPTETCTIYLDIIQSEAQIFQYCLLLLIVLIQVQLPFSITIYVFWFQEPQTKQDSKSIEFEEDYFKTIKLQVKTDSNSSEQITTLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T1R5</accession>
    <name>A0A8S1T1R5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0140226</name>
        <name evidence="2" type="ORF">PPENT_87.1.T0140228</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T1R5"/>
    <dbReference type="OrthoDB" id="301788at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8145405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8145409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="362" mass="43512" checksum="DD5D40A29FD73E16" modified="2022-10-12" version="1">MNCSVFSYYVEQTLPNYVQQKLEECFVQLSNNQNIIMCVDKICEQIEFCRKLCNRLQAQKQIFTLIKLLEHQIYPIKDAEKNNTYLLSLQDVYVFQIILRMLCIMIQFESKKYHDNFDDDYTEKFSNDLVKGFLILRTNQANQLDQQLLEMFLKLKSRSILFYKYLIKWSEQFQLKNCFSSLRKLKLQLKKRQTEQMIEKRRQIDTIKQEYKEECSNSFYEQNDFRIKDKRLQMIMNVKQPKQEQQFISFQDILRQKENTKFINTTESTLSRPSLNKRTINGGINGKYKLGNEIKVLRKTTSSSSKKNNKAEMQPFQILQQQYQLEFCKEVSRKLLKLQQKKQMNWNSEYLAADTESDSCFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T2U4</accession>
    <name>A0A8S1T2U4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Casein kinase I</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0150143</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T2U4"/>
    <dbReference type="OrthoDB" id="286981at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000616">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="13"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="329"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8146038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="512" mass="59390" checksum="BB8B02D01C77B893" modified="2022-10-12" version="1">MAAVSQKIFNNQFVIQKKISSGSFGVVYQGYDLRSSDHVAIKVEKQEIDDLFSLDREIQILRNLQGLPQVPKLKWAGKDQGNNVMVIQLLGRDLTHYLRQRKRFSLACVLGIADQMLTILEAIHSKGIIHRDLKPENILAGKDREQNIIYLVDYGIAKQFKDKDDKHIPFRENKPFLGTSRYASIAAHKGQELSRKDDLESLGYMLIFLLKGSLPWQNISYKNEDEKVKMVGLMKMRITSQELCQDIPTEFMRFIDLVKKMSYREKPDYRYFQQLFRRVSIQQQIEYRFDWYDESNFEKKSGSTSPKKQQSIKILDQSYLIQQIYSPNKKKGSEEFDDTGPSKRESNGRPSIINNNSNNLLMCDSRMNLKNKSVSSFNESNLQYSDIQPDVSCLIAYQRNLRYGSFHNEVEIPKQPDRSTSAQDKIKVKHKLSLDVKPNCYKSLVEFQLGLMNNPSIIDFTVNSQNQLNEDESPCLDDKFNILKVSSVDNQFKNPIQLFRLNLKDRTGIKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T317</accession>
    <name>A0A8S1T317_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0150038</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the inner dynein arm light chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8145837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T317"/>
    <dbReference type="OrthoDB" id="273640at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019347">
        <property type="entry name" value="Axonemal_dynein_light_chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13183:SF0">
        <property type="entry name" value="AXONEMAL DYNEIN LIGHT INTERMEDIATE POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13183">
        <property type="entry name" value="AXONEMAL INNER ARM DYNEIN LIGHT CHAIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10211">
        <property type="entry name" value="Ax_dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0243">Dynein</keyword>
    <keyword evidence="3" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8145837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="183" mass="21664" checksum="92BDFA73754641C0" modified="2022-10-12" version="1">MEKQQLWIQCVSSTPAKREDVILLQENLDKKLQQRQARETGICPIREELYAQCFDELIRQITINCAERGLLLVRVRDEIRQTIQAYQTLYESSIAFGMRKALQAEQRKTDYNNKIKQLETECQDLSKQVEKIESTIEDMQRQDQEQQEHEEAKHKDQVNFLKNANKVYKEELEKFLTGANVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T4F7</accession>
    <name>A0A8S1T4F7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ceramidase</fullName>
            <ecNumber evidence="1">3.5.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0160042</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.23" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8146768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T4F7"/>
    <dbReference type="OrthoDB" id="5273684at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017040">
        <property type="term" value="F:N-acylsphingosine amidohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28583">
        <property type="entry name" value="ACID AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28583:SF1">
        <property type="entry name" value="ACID CERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035743397" description="ceramidase" evidence="2">
        <location>
            <begin position="16"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8146768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="360" mass="42390" checksum="B801C3160578640E" modified="2022-10-12" version="1">MQYSILIVFMSFVASFNYLPEVEIDLSIEPKQRWKQAIRIIIEQYGYENSFGQVFEFHNKNTFYILDPQDYSTIAKAIRIHYPEYSLELDGIVEELNRSEITFEYLSAWAYFHEIAHISTDITESTGLLLQVGDQIIHGRNMDQAPIQARNIILHLIIKKDGQYLGESVEWIHYFIKIQCCILEENWRFCIPLNKNQLMIFIEQGVSSLAWTYRYILINENMNTFDKVVSYLEQNQVASSFYNVIGGKEKNQGVIISRDPFIMYPTISLNTSILNQINYQYLVQTNYDHWLPDPQNDQRRTMAENLLAQLQIHQWNEFGVFAVMETYPIHNEGTFYTIIMNAKYNRLIAFGQPNMTQIEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T5R0</accession>
    <name>A0A8S1T5R0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0160091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8146866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T5R0"/>
    <dbReference type="OrthoDB" id="68328at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011:SF28">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="43"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="183"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8146866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="407" mass="48317" checksum="0D18AF94BA23F85D" modified="2022-10-12" version="1">MNISENDMYSLILAINKRSMFKTQYDIEFIHSKLQDQPFFRNMHRQIGDTQYYKILKELQYECHLPYEPQVQIGDQSTKLYFILAGKFLILTRNHQSNIQLPTDIQKKLDSQYANLIPSGYLYPGQYFGEQVMIFDQSHTQTVLPLEKSHLIVLKRETYRRIIEQETKKQESERFANLCQIPLFQKWSAKTIRNLISEIQELNFIPNQIIYQQGDPVDSIYIIIDGQIQLYRTYNKNSLPLSIVGSKECFGEDEILTQFRSHSAKSLTSVKLYRILKNKFLDHIPQDYTSKYFKKDSHHKLSIYQNEDQFRIYRSESLKNINSPAKVMQRILTRNKEKNKTHQSISFQKINELMKKDFRQSNTSTILQKSNSVHENIRIHATGKFDKTNYLLSHIRMRSTSLWKKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T7K4</accession>
    <name>A0A8S1T7K4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0160185</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T7K4"/>
    <dbReference type="OrthoDB" id="299791at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="244"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8147054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="527" mass="62923" checksum="8B17A14D2FB942C3" modified="2022-10-12" version="1">MSQHITNNYFQHFLKNDSQRRIKTLNSINDTNSTKIKKVIIPKNICIDKETLYEQLYHEKLRSKKLSVENQQLIGVLSQLESNINIMDQTDQRKMLSQSFLKIKQQDQEIQTLKESIQYKVQVELKRQIQDLQTQLFKYQLSSKFEGDQYQLIEDNVSLLNKLESQKQYIIDLEKTRSEYILIKAKFNQLQQVLKLKEQQIQRFRDRDPLSEMNLLRNPTKNENLLMNELNVKKDEIQLLTYKLDQSQTMIQVTQNALTQLNQESKHKIQILENEKRILIERLEKLQLDYNSILEKQKQMDKYLSQFQKKKIQTSPFVYGEDSLFQSATQLPLSPTATSNDQLIVKQVRKQQIEQIVLELKLSLRKKRITLQEAETILFSNESEISINEIEKKLKEDPFTIKNSTLLARYLVEDYSEKEFVYDPDLKVPQAQVKSIFKNLLQNYTLIFDDQVIETVKIFIKHYLQDYCQKKSLSIINQDSIIDCMQLYEVKWNGKHSDYLQQEYYNKYSKCQEYEINNLLRLFEDQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T7Y1</accession>
    <name>A0A8S1T7Y1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0180033</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T7Y1"/>
    <dbReference type="OrthoDB" id="304726at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8148373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="351" mass="41956" checksum="8026EC91CD9B3461" modified="2022-10-12" version="1">MKNQDEKKYILDRIGTGSTMAQTGQQSQFQQQFQCEFNQPTFGKIKKEQNYKINNRYQKQDINDDPVEQEQQMRDNQNLQEIQQLQEKDVQTKQKKIKEDEYQKLIKFNEDNYNIDNQYLEPEQESPIMKPKKVKGDHLYIACQIKPFGEFLLKYHLQDIAHPYIKKRTTTKCCFFSVNTKQSLYQTQKQNIIKVGQVLYDDANEDHCNLLYSIFYLIFQLEKLSKRKSQEISNNKINLFQLLYTLSCVTSFRDDLFQIGKFDKEKIVSIMFICSKYIFEVLQYGSLDPIFKNTESNGETFHNLMTCIHMGVFRYISNSNYIQEKEIYQNLQNKSVKQLLDIWKENKFKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TAC5</accession>
    <name>A0A8S1TAC5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0190070</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1431934at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097039">
        <property type="term" value="P:protein linear polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20335">
        <property type="entry name" value="BRcat_RBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22585">
        <property type="entry name" value="Rcat_RBR_DEAH12-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000041">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051628">
        <property type="entry name" value="LUBAC_E3_Ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22770:SF13">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22770">
        <property type="entry name" value="UBIQUITIN CONJUGATING ENZYME 7 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="1179"/>
            <end position="1384"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="1183"/>
            <end position="1230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8149360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1389" mass="163904" checksum="2CC555E7EDACE568" modified="2022-10-12" version="1">MQNQQHLIREIYLKDKSNSFLRQQWVQVFDSLLPLQQTTLIVDTLQEIDTLKFIKELLSKNHAITVISDIVEANYNWCNLVNQNFEIIVADLRTETKLNSYQYIGIKWLLTTKSQLVIITNDLISEHLINYFNIDYDNIIEIPYLQTQQDELFLSIPYNQKLTYVLQQIKNSNSEIDRDIVVYCESLYQMDQIFQAIYFHNQLSQSEEFEVHQIIPFQQYYEQFGQQLMEMVYPQQKGKRKVFLTLNYQQLRKYLQNVQSKISLIIHMGYQTTVKTFLEEINNAIVYKQYNLSRHDIIIRNRISYYPKIIYLFGFDEFKDLQEHKLIFDEEFTFNPQNTKFYAQQFNIQIDQLQNVVDSEYSSLQNYIPLIYYEPLLEAENETFHYYSSVISTLLTDGEILSEGNVESKKNPFKSFQIHQQRESIIRSNHYWSKMGDITSWIQLINKFIIFIEDINSKDDEKEQENSKNELIKWCRLQNINIPKIILILAYNEYIIDLTQKYVSEYSVINKCCSLDQLKLNRTVSLKKQQSMPEHSEDIQACLLRLFYKGLSIFSGHKSAGYYNYVTNQKFKLCYNNLMAIVGDFPLTCIIMALLQKNQQYEIKYSTKIDDIMIPQLAPKEFVKEYKLQFIQRQSLHLNEINFNGIGQVLFNELWKHQSGPQLSQDCILQPNIEMNCITVLYDPNVAKKEIIEQELNSKIKELQNFYRHQVKEIPYENDSKFIMQAGGSMKDWIEKSAYKTFFIEGLPLDITQELLQEFLPEVIISEMKLYKEGDKITARISLQDHDDLLFCLENIKEYKDNQLKVYTQQDQFEHSEEKLLFNEHYKIIITWNLHLNKGTAIVKLNTPYIFNSEDFETIQHLTCKMNLKSVYEDEQSVMSDLKQQYKDIDIKSILIDKQLRYENNGDLQLEIQKLLSNISQDQYKIESITKQNCKRILTLIVYDRNIMEQFILLDNQIKQFRGLKVQLNVKPVYYHDYEGPIEMKQIVEEIFDEKNIKLGQYTISSDDEFKRVKVTIQCWSKQDHEQLNKEIQDAFTPKIIKSSQDFDITVIFSSSGTRFLKYQEESLQILIRQDFINNQLFVNSSFIKYNQLVKAIKEFISDCSKSIIQIPKNCIRLIMGNDSQGLNEIKKNFDLKNVKFNSISGELQLFGDQKNLDAAIISISEIMSSQQQQDKPINALTCNYCFDNMKNGYMLQGCGHKFCLQCIMFSIQNSLGDMTQLPIKCPQCNQGILLADLYILIDEPSWEKLIKLSINKYLQDHAAQIAFCLTPNCPIIHFQKIPRYTCKKCQKQYCNSCRTAYHFGQTCREFKAGNEDSINIYMKKNDVRRCPHCKILIQRIDGCYRVTCTGCKKSICWKNKADGKPCMEIFETSSECYSHLTKEHGGYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TDK3</accession>
    <name>A0A8S1TDK3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cytoplasmic dynein intermediate chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0190213</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TDK3"/>
    <dbReference type="OrthoDB" id="4189at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010970">
        <property type="term" value="P:transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF22">
        <property type="entry name" value="CYTOPLASMIC DYNEIN 1 INTERMEDIATE CHAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="25"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="26"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8149644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="582" mass="66116" checksum="E6CB7ACD7F02F071" modified="2022-10-12" version="1">MISQQEKQRLLQEKRDRIAAYKQKLEQSQQISSNTQPQQVIQAQENVQTNQSPQKEQPLQNQTIITHPQQSDLISLYLQTKRKKLGFQTIEMYSNNPAPPQAEVMTIEFGVQCEFEMPQSKQENDDQIIPSMENVRRDSRKKREVLQSAEKPVEKVEKNISMEEINSIIQSQSFQRFFSRSSKTIEKAIHGVEVDVLEDLMNTQQVAQEIGKDNLTKLLSFSDKLYTENRVVTSIQWSPNLPNTFLASYSQNEEGSITDQVGVVLLWSLQLKSRPEFYCFASSPITSACFHPFSPNIVLGGLYNGQVVVWDLRAKHTPEKRSTLNSGGHSYPIYGLNIVGSQNANNLISSSNDGRICIWNLAQFNQPQKIIDLKTKNKQQSTNPQVEVNATCMMFPQGDANNFYVGAEDGCIYKSKLHQNTNENIVIPFEEHLAPVSGISLNSSPQASPIMSNLLLSSSFDWTCKLWNTRTQSDSKCLANFECSEDYVYDIQWNGQHPSLFSTVDGEGYVDLWDLSYDLEAPITRYKSGNNSINKSQWSKDGTKLAIGDSFGEIQILQLSQKAQKIDHERLAKLEQWIKSDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TFU6</accession>
    <name>A0A8S1TFU6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0200376</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TFU6"/>
    <dbReference type="OrthoDB" id="1668230at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001175">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000683">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="110"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8150623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="524" mass="61320" checksum="5988A1E86E3C8DE9" modified="2022-10-12" version="1">MQNINYQESKFQFQCFRKHLLIDTTYYGYLLSNQLILATVKQSQTPKYKVPLLLTTQIHFITKQPSNEIEQFGFLYKEQFKYFYANSNTILQLKELMACKVMFKEISDFYQPISLLGRGGSSKVYLVIKKRTKDQYASKCVDKRYLNEDGGFNGLFNEIQIMQKMNHPKIIHLEELYEGDNTFYLLLEYLQGQSLNETFNKRQVLFEQEQIQTIMFQLLTAVQYIHSLGIMHRDLKPENIMFKSQNAFDELKIVDFGLATSIQAETYPYPKCGTPGYVAPEIANLKDQNQKYDLICDMFSVGCIFYKLLTGKELFTGTDYQEILKFNKKCQINFELLTLYRAPKEAIELIALLLKINPKERITAQAALEHNYFQLKFQTKRGKFQQNNNKYTPVFQTQNFNPKDNPQKQNFHIEDDIVEDENAHSLKVPVMQNMKTFGQFVCKIKSQVTPTLARRNFKKFQTSDFNSALSPNSPNPLLSEHKPSLIINAQTQEFINEVHTKNEHKINQMGQIDEEQEDQLQQNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TI49</accession>
    <name>A0A8S1TI49_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0220008</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8151598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TI49"/>
    <dbReference type="OrthoDB" id="309930at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050498">
        <property type="entry name" value="Ycf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE SPINDLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13431">
        <property type="entry name" value="TPR_17"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="6"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="40"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="74"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="108"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="142"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="176"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="210"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="244"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="278"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="312"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="346"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="380"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="414"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="448"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="482"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="516"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="550"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="584"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="618"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="652"/>
            <end position="685"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8151598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1018" mass="117756" checksum="98102A60AF1C55B7" modified="2022-10-12" version="1">MFAGGADAYFNRGNLFKQQGEKEKALQDYNQAIKLKPNDASMFFNRGNLFFEQGEKEKALQDYNMAIKLNPNDANMYFNRGNLFKQQGEKEKALQNYNMAIKLNPNLAEAYHNRGNLFSEQREKEKALQDYNTVIKLYPNHVNVYNSRGNLFLEQGEKEKALQDYHTAIRLNPNFSIAYFNRGKLFSEQGEKENALQDYNTVIKLNPNHANVYNSRGKLFLEQGETEQAFQDYNTAIKINPNHANAYINRGKLFSEQGEKEKAFQDYNMAIKLNPNYANAYHNRGNLFLEKGEKEKALQDYNMAIKLNPNYANAYHNRGNLFSEQGEKEKALQDYNTVIKLNPNHANVYNSRGQLFLEQGEKEKALQDFNVAINLNPNHANADNNLRNLLIEQGETEQALQDYNQAINLNPKFAIAYFNRGNLLIEQGEKEKALQDYNIAINLNPKFAIAYFNRGNLLIEQGENEKALQDFNVTIKLNSNHADAYHKRGILLKLQGEKEKAIQDYNTAIKLNPKFAIAFFNRGNLLFEQGEKEKALQDFNVAIKMNPNHANAYNSRGNLFLEQGEIDLALQDYNIAINLNPKFAIAYFNRGNLFFEQGEKEKALQNYNIAIKMNPDHVNAYNCRGIIFKQLGEKEKAMQDYNAAIKLNPNHAEAYHNRGNLFLKQGEKEKALQDYNKALQLKPDQPLFLTNQGSLYFQQKQFDKANISFIKAQSLLDQANEQIFQWNLSQSNVTYIKKELNLLREIQIQIQTTDLQLEFISQGLQKRKSIELMEQASIIVNELVEDIKPIDTLSIEELNSEQIQIFLKKLNDLQNQILSLTLNVNQLLEQDNFKVSEFLKELNKEKNRNQQMYYNALNWRLFNYLSAMQQISTNLFQINSSTVIESKSEKTLNIFQRMLNIGSQIFGGLPIIGNAFNIINAALDFGFEQHKEHKFTARLKKLNNILKCSSIVPGQLQIEIQIASQELSNNQLNNLIDKPQSRFRRFADQLLQEEDKQFRDNIYWAACIDYFKLFGNKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TQN2</accession>
    <name>A0A8S1TQN2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Importin subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0250028</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the importin alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8153782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TQN2"/>
    <dbReference type="OrthoDB" id="428811at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000009">
        <property type="entry name" value="Importin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032413">
        <property type="entry name" value="Arm_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024931">
        <property type="entry name" value="Importin_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16186">
        <property type="entry name" value="Arm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005673">
        <property type="entry name" value="Importin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="135"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="178"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8153782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="516" mass="58158" checksum="82E195F474B0E633" modified="2022-10-12" version="1">MQPQNQFSLFGNVQVNEAEQNSEVKKKREEFATEIRSRERNNIINNKRLAQNNQTTKIPESLPLIPPTLPQDLQIALKAQQEQLMKRSFSFEDFPDVLQKIFSTDILQLHYGVTGLRKMLSVESGAPIQQVIDANLVPKLIDMIQKEQIPQLVLEAAWALTNVASGTTQQTQSIIDKGGIYLFVKLLSSQYRDIAEQAVWAIGNIAGDCTQYRDLILRVGGVDPLIAIMQNAPNKNTIKHCTWSLSNLCRGKPIPDFKYVKNALPVFCKVIIDETDPEVLTDACWALSYLSDGDNSRLQTVIDSQVVPTLIKLLDHSSLQLVIPTLRILGNVVTGDETQTTYVLNQGLLLKIPKLLSHDKKAIRREACWTISNITAGSSNQVSQVVRDSAILEKLFTLMTTDVEEIVREATWAISNSTKHGSNSDIQLLVQKGLLNVFKQLLEGEDTQTMTVVLEALLNVLKRGDQDFKDDNYYLTQLEQMGVISKIEQLQKHQNQSVYQKCFNILEQYYEAESEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TW49</accession>
    <name>A0A8S1TW49_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0270015</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TW49"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8155299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="158" mass="19200" checksum="C983BCCFBAC037A8" modified="2022-10-12" version="1">MILVNEINQSKKYVIILEMIKQENYMKFNKLFKEMLYFIIQIKELNEQNSDNQNLRDYYIIKGTIELKIQNLIEKDSQYESKILIDQKINSVDKQLNQKVNWIELQFKDEYIFLIHSTSDWIQNFKSSAFMSSLLRQRCYKIKEGSFIFERRKSQSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TWY4</accession>
    <name>A0A8S1TWY4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0290144</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TWY4"/>
    <dbReference type="OrthoDB" id="10589219at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="166"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8156738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="319" mass="37673" checksum="F8926A941275195F" modified="2022-10-12" version="1">MSELQNKYRITSRPFSADKILITLQNLDYNTQQQNTLQINHLTTIQDVKQSILNNLEHLQNIELYFQQNQPFIGNLNELVLNKIKQANTRIIYYKVVKKRNNSQPQNSNNYQQPKLVITVQEQKVQQQQTSVYQKNMITPAQSNPQNFLPQLSNNQGNIIFNTPKNQPLKNTLQKLQIQYNDLEKQNQELANEKKLIQKQILKNSLKIQDLEQENINLSKLNQKLTDELALLKYNQSEFENLKFENQQIKDQINQIRLNSEQILMEKNKLQEQISKLVLYQFLLNKAKNVSQIISNKKIINLIKQQMIIKNNQIFFLTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U205</accession>
    <name>A0A8S1U205_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0320025</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8158558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U205"/>
    <dbReference type="OrthoDB" id="25620at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF122">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN SKYWALKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="2">
        <location>
            <begin position="656"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="421"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="500"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8158558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="830" mass="97702" checksum="72D6815DF5A43487" modified="2022-10-12" version="1">MDEKDFEKRICEKHSYQIIAVDLKLSKTQNEKYLCGKCLILRIEGQQILLLSEMIEMIKQMKNDQKKQIIENNQTQLNYMKQLQQSLKECNQHIKEKFEKTQNNIELQIKDGNKETNKKEEIQEEVNLDKDIEALSKYYKGNKEYELPQGEQNLNSISQLIENVQNQIGSISNAYQYQKILESLQNIKIQFQINMSSDKDIKICEQHGLEIIMVNIEQGQQEHKRIACSKCIQEIPQNYTTLEDADENWKKFKLKSKQMISKYSKDRESKFNQAIEFITNLRDKYQEILTTIINDLENNRVVTEKQLSPFPLYQKNIYQLDENQLKEILEALSQKDLYLQLKQKLAQQDQLDSIFYHKLKETLENLMKYDLLTKEQIIMIENNYKNFLILIEDEKQHISSCDEIKQFLENTSLLQSYMNIFEDSIQLYQNLQNQVDSLNEKSDIFKQLKQKQKPKKSGKQMDQDHNSDQKQFFQRQYTLFDQISKKYKQLLIVYKDQNLLTQLQQEKSELSNRLKQEINIQTQLKENIQKLENQIQNLQSDLEKQKQQLEKTLVNLDQKNESEKQLMGKLQQETQKFEELKKKSDEQDQKNNSTIELLNKTITDLNSQIKNNQTNLQSKDQQITSIQQQLQNSQNSLNKLQQTYNDLIKEIKAKPNLLSDAYFQKILQTIEEKSRSVIKCSILVYCGSKDGLNHTSFWNKVYGRRNLLMVFKSNTNFICGAFSPCQWLQHLGNYVVDDTLQSFLFSQNHNQIYPLKAANKAYAIYCNSSYGPTFGSGHDLYIHANFNSLSTSLGSSYQCDQYGSITGSTHLFSQSSVQIIECEIFEIVFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U5L3</accession>
    <name>A0A8S1U5L3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0320238</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8158947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U5L3"/>
    <dbReference type="OrthoDB" id="532484at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029147">
        <property type="entry name" value="CFAP77"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28617">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28617:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14825">
        <property type="entry name" value="CFAP77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8158947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="205" mass="24043" checksum="E1876129E0CA48FD" modified="2022-10-12" version="1">MKPWNKNRANSLLLKDSVGAAKQSIYTIPNDIYFGKAIIHDLEGAQQVTSTWYYHNHSEQNPPDRDFMKLNKMCISSKLHDQKQFYLFRKSNDAKVFRRRGCSQIEMNLPDENFRYGKPYLPQSPMKNVLSGSYMNEAEQLMDKKYDAISKYKQQQNKRPSTATKHTKASKLAYQSLTKSLNKTQQPQFKLKEFDKVEPKTKTRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U7X5</accession>
    <name>A0A8S1U7X5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0350238</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U7X5"/>
    <dbReference type="OrthoDB" id="674948at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044280">
        <property type="entry name" value="Hac1/HY5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46714">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR HAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46714:SF6">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR HAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="9">
        <location>
            <begin position="81"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="43"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="92"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="71"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8160920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="260" mass="30479" checksum="82A95F531861527F" modified="2022-10-12" version="1">MSTSQQELFEYMSETLQTSEKGLLEMQKSHPQNFQKLVEKMKRTMRREKAKQRKQKDEPEIVSSSKKIKRKKEDDDPLRENDKKTIQMIRNRISAQNSRDRKKQQLHQLESQAQKELDYNAELRKQLEELQEKHQKLTNKSQKIRDQLSILQQLGRGSRLGKISLALLTLVSVFSVISQKEAHQTITTPLVKIEEISSQSALRPINYEKSYSIIDTNTFFEEEKLGVGISEESENCYNQLLPIPLYRLNSQIEYALEQHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U889</accession>
    <name>A0A8S1U889_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0330274</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U889"/>
    <dbReference type="OrthoDB" id="407558at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000025">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase CYP19-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="5">
        <location>
            <begin position="23"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8159689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="186" mass="21011" checksum="CFF59BBAEB475F46" modified="2022-10-12" version="1">MLTDSSLFYSFSKQTAVKVQLVFLDIKIGTDKPKRVIIKLFYDEMPKTCENFRALCTGEKSNPYVKLNFRDVPFHKIYSNFMALGGDILNKDGTGQCSIYGPTFKAEPKRFKHDQRGLISMFNDGNGNIGSQFFFTFTDCSWVDGLHSVFGKIVEDYSILDELEKISSTNGAPKKLVRIVDSGVIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UG84</accession>
    <name>A0A8S1UG84_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0400095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8163850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UG84"/>
    <dbReference type="OrthoDB" id="285450at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8163850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="178" mass="21082" checksum="619E8012F97B518B" modified="2022-10-12" version="1">MTSIEKQPYYQDPSFQFFVKKGLIKQNKKGQVFPIFTYRVNSQPSQSKYIELRVFPSKIVKRSNIQQNSPSPFVITNIPKKNTLYQTYIDNIIQKKTKDKSESRKRYSNSIDEIKGKQIETVKDCFLPKLHCLNNKPQRLQYRHSLPKQDIYTEPNFEPNPSDRLQPWDSSQKSFTLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UIN8</accession>
    <name>A0A8S1UIN8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0390308</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UIN8"/>
    <dbReference type="OrthoDB" id="312555at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="268"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="681"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="554"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1075"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="681"/>
            <end position="715"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8163612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1113" mass="131184" checksum="F2BFB0A2EA8753CE" modified="2022-10-12" version="1">MKPYINAYPITTYNQNIDVMQTQVSSNLQDQINLLQEIKQLLDQYPHQNITRIIQLTINNNTIESKHETCDFTLDQYLKNRQSFWLSKEEIYNILGQIVQGYMHLKNLKITHRDLNPRTILVLRVLETNNVIIKISDFGVASLNQTRMRENAGTPFYQAPEITQNNEYDDQCDVYSLGLILYQMCFKNQYHIYPVTQEELQKFQEQLKFNKFRIPDGLIDPALKQLIEKMIVYDPNQRINWSELANMQVLQPKFLILPNQKNTKLYYIDFLKSLGRGSQGCTYLTYDLINQNTEYCTKQIDSNNIEGQRELQAYKLIKENTYCENIIMVEDILSQKDNSYLVMEKCEKNIEQYFREKQFDLSQQEALDIVQQIVTGYLHITKFNIIHRDLKPQNILIKHNNQTNKIIIKIIDFGVGKVLQQSKELTNTYAGTPIYTAPEIIFSHDYNNKCDIFSLGVMLYQLVYEGANYFQGQPNSIEDLKRILLNIKSTPVQCKKPYKNFNNNFLSLIDKMLIFDQNERIDWETLNQQLKNFTQSKIKIISINNQNLDLQSQTQQEQLQQQQYQQQYQQQQQQQQQQQQQQQQQQQQQQQQQQLFQQQQQQLFQQQQSFQQQQPFQQQQPFQQQQPFQQQQQFQQQSPFSSQQPFSQQQPFQLQQSQQIQNQLLLQKNQLPSSFQQQQSQQSLIQKINPQPQQQQSTISQSFSFQQDSNNKSFQLTTPKSNFIDQNQQFQLHNQSQTASQTQKSSTLAQQSLTTLRSTMFQTHSKTPQQFGQPQQFQLRDQLKTNPPNQQENKASIFPQNTQNQQIAQLQLDPKTPLPQNQFAIQQNKQSDLNTNSLSPFQPQQQQQQLKLNINPLIVNQMNPQVAIPNQFSNINTQQSNQLIDVSKPISCRATISYASTTLNNNNNNNINNNNINKINNINIHNNKPDATKFTTQAIINNFPSKQFSSKNMEQFYQFTKKASEDFKTKSQQLYIQPAYLGFYLCILYFWQEINRQQKIEKSTDSQINSEELTIKKDIERVKEIISQVDKKNGRLEDLKRLILLQNQLQSLAQIQQAYQEFHTKNHDILCDQNNNKEQIELNKIIENMHNLSKDLNSNKQNTDNQKFQFNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UJ37</accession>
    <name>A0A8S1UJ37_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0400051</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8163762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UJ37"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="60"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="96"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8163762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="306" mass="36332" checksum="11C1A8169201BE7C" modified="2022-10-12" version="1">MNCFTKFKNLIQKQNNIYFFMQILPPAERVLHFQETKSWFNSRHSSKVKEKYRKTEKELFDDEIVTETFHLIDRDHSNTIEMDELYSMLKKNNYPVNLGLLKAFFEMTDRDGNKCIDLDEFKQVIKDEGTSQTFRTLMRKMREIGEENYYSTDFVNLLRYLSYCANRTDLIWQIKNTRLSFEQRSKLVSELFKVNQQFTKISNPKEEQKCTLRPFMKNKVQGEIKYLTPINKKPRIITNMSTKTRISTSNQNSILSFDINSISSPRLSPLKTDRYVFSKKMSLTTTPSTKTTLYSTKSTQKINNKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UKC7</accession>
    <name>A0A8S1UKC7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0420142</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UKC7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8165300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="321" mass="38091" checksum="FDC4539E9CE7A614" modified="2022-10-12" version="1">MVQFLVLVPYNGDYQNTKKVGRWDTFFKCGDKGFTQMLILLQLTVNLLSIKIGRWIELSYAFKLDSQVIYKGEYKKGIKLADGIFNLEEKIMKNLNRCKINIVNIMYKFAVVKDHIMIKLKEAQLRLGNGLSRMMNLFGIDELLLMTNIQIIKKLVDGIFGRRILRQKYINKYLEQKINLQWSGSYDDDEDSSKIGQWIDLSDESNCYSQITFRGEYKNNKKVGRWDTWYKDQETKKNEKMSIKQNSTGGGSYNEGIQIKQWIEFSDRSNAKFLINYDGEYINGKKVGRQDIYWKKNCEGKQKEPMYQQQIFNYKWWWNIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UP22</accession>
    <name>A0A8S1UP22_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0420241</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UP22"/>
    <dbReference type="OrthoDB" id="298264at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8165492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="241" mass="28590" checksum="130338FB4A0160E4" modified="2022-10-12" version="1">MGNFCQGDKLNDLNFQDNTHVYEATINLSTITIIHGNILLNNLRGILNAQIDPNTFTLMQLPEMQREFLKPKIPIIQRLPPQSCQFEFIINLKINYQDNRKEIIEAYQQGFELMFQYELENVGYREGKDKFKDKSEQLHNSECLLEAIFNQIKQNKMIKTFQIFSVDMQICQQYKNELKKQQHKYQSKKNFERCSTQEQLNESYTERICISNSCDQADIINKNQIKQQLKRSFPNTQQKDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UQ57</accession>
    <name>A0A8S1UQ57_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0430179</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8165892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UQ57"/>
    <dbReference type="OrthoDB" id="295249at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8165892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="68" mass="7716" checksum="38E9D5FC698FFD1A" modified="2022-10-12" version="1">MGCCSTKGRRKHSLSIPKTSILKKPSPLQQVQKEIVNTKKKTLSFQLLSINSQEVNRKSLYTKQIISD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1USB6</accession>
    <name>A0A8S1USB6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribulose-phosphate 3-epimerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0420162</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1USB6"/>
    <dbReference type="OrthoDB" id="338816at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180022">
        <property type="term" value="C:RQC-trigger complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004750">
        <property type="term" value="F:D-ribulose-phosphate 3-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06554">
        <property type="entry name" value="ASCH_ASC-1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00429">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.130.30:FF:000006">
        <property type="entry name" value="Putative_zinc_finger_motif_-_C2HC5-type /ASCH_domain_containing_protein_-_putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000171">
        <property type="entry name" value="Ribulose-phosphate 3-epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02227">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007374">
        <property type="entry name" value="ASCH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026019">
        <property type="entry name" value="Ribul_P_3_epim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000056">
        <property type="entry name" value="Ribul_P_3_epim-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039128">
        <property type="entry name" value="TRIP4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009349">
        <property type="entry name" value="TRIP4/RQT4_C2HC5_Znf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004076">
        <property type="entry name" value="PRK05581.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12963:SF4">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12963">
        <property type="entry name" value="THYROID RECEPTOR INTERACTING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04266">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00834">
        <property type="entry name" value="Ribul_P_3_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06221">
        <property type="entry name" value="zf-C2HC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01085">
        <property type="entry name" value="RIBUL_P_3_EPIMER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01086">
        <property type="entry name" value="RIBUL_P_3_EPIMER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRIP4/RQT4 C2HC5-type zinc finger" evidence="4">
        <location>
            <begin position="3"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="ASCH" evidence="3">
        <location>
            <begin position="271"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="201"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="210"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8165336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="871" mass="100318" checksum="91146D4F9FA2882D" modified="2022-10-12" version="1">MSRKQCLCEAQIHKLINNCISCGRIVCSVEGEGPCLFCGNQIMKKGELLIDDSQFPDMESKTAYSKALMHKDKLLDFHRRDVHQSNIIDDQADYYQIVDDVWQNEDIRSQAVIKLIQQADAEQTNTVKQVLDLQTMQFRLEKETNAKQADEFRDEAKQLLKEAELADKKKNQNNSKTSHLADNQEYQKLYNEIITNLNNETKKESKKQNNKNNKNNQNTQQFKPTSCRVQFTDEYFQDFQKELLKVVNQEQQQEDIFDESFFKVEDGIGCLSMHQPWAQLVVEGFKRIEGRMWGTNYKGPLWIHAGAKQPETELISAIEHQYSQLYNNLDNIPQFPARYPTGVLIGVVDMVGYVRAAEYNKMVPKEKQEEGDTEFKFVFRNPRKLLIPIQMRGSNNIFKLDDDILKIAIKQLEKVPVKWGPYLIDPKDVAKEAIGEKQVDDTMSKIQEQFVINNINEIQEKSINEQQQKVENSIVTDQPQQQEKRYQQVQSIDSQFEKQLEWGILIKNVLKRDQLVRMANFLSTKIKKQNNQKGALKLSFMAKNQLPCHDIITNTISNITGNENQYDNCTIHFLKLADKKICIQNATALILIGQSMEFMNNNQQILLQQGKVFLVEEGYTLNGQFSSDQQEENLRLCIQNLVDIIIIMIKIAPSILTCDFSIFYEECDKLLKMGVDWLHLDVMDGHFVPNLTFGPPIIKCLTNRLQNRTFIDLHCMIQEPRKWIKDLSNAGGHQMTVHYESDIGNIEELSQMIKNAGMRPALALKPKTLIDHTLIEIFDKQLFDMILIMTVEPGFGGQKFMADMLPKVTQLRQRYPKIDIQVDGGVNCDNVISCYEAGANVIVSGSGIVNAKDPQQSINYMKECGCQHFKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UTU7</accession>
    <name>A0A8S1UTU7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0430176</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8165886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UTU7"/>
    <dbReference type="OrthoDB" id="2414662at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004838">
        <property type="term" value="F:L-tyrosine-2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006572">
        <property type="term" value="P:L-tyrosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005958">
        <property type="entry name" value="TyrNic_aminoTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01265">
        <property type="entry name" value="tyr_nico_aTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45744">
        <property type="entry name" value="TYROSINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45744:SF2">
        <property type="entry name" value="TYROSINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="417"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="7">
        <location>
            <begin position="71"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8165886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="644" mass="74222" checksum="01D92B67AA9AC713" modified="2022-10-12" version="1">MQQMERSWDMLKPSARAQVSLNPIRNWVEHVVPEVEKAGKKEGRPEKKIVFLLGDPTAYPKFQTPNEYKEIAANSVGKIDGYTDFFGDFKVRTQLAEVLSSHHRKLEADDIILASGGSGALFYATLALAKPGDKILMPRPTFPLVKAFADFYGIQVVFYDLNPGTWQVNILELEYIYEQNPDIKFILVNSPSNPMGSELSPQALKEIVNFCERYNLPIVSDEIYENMIFEKREFKFISDYTKTVPVLRCSGLTKKCLVPGWRLGWLALYGEGDTFKQVKQALRNISNILLMPNTICQAALCEVYKKSLDIIPEKMEELHARYKALHHGLHDAYGISVGETKGAMYATLIINTEEFSDINSSIDFAKKLQQEQNVLVFPGELFYGEKFVRLVICCDLEIIEEACQRIKKFCLDHKKVFVLQSLLCYILTQIIINIIILIFIHSKFFMDSKSLKGNRSKKYEKTTSEERAMIILMKENGVSCKEISEALKKNIKTIQSIKSIEKRSEYGELKKALIEFGYDWVIQNKNLNIENTRKLAKSVNRLIKEQLTSKTSQFQKLIPEHKILSNKRRIIQQIVDSIIQKVFNSKQLLENLETTNQQKIIPKEEEPDDQNKEYLNFGQLTILQMYYAYTQFIQQSIQQNDLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UUG7</accession>
    <name>A0A8S1UUG7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0470158</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000047">
        <property type="protein sequence ID" value="CAD8167512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="340346at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051023">
        <property type="entry name" value="PP2A_Regulatory_Subunit_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648:SF4">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2 (FORMERLY 2A), REGULATORY SUBUNIT A, BETA ISOFORM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A 65 KDA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8167512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="275" mass="31338" checksum="A6BCF0E1E7C74C74" modified="2022-10-12" version="1">MDEEDCTIFIEELKSDDPNLKLNAVSKIVSIAQILGPSRTCQELIPYLIDIIEEQDNEDEFLIKLAKELVNLKPFTGANVHLLNAPLEILSSMEEPLVRDKAVESLILLAEGMPNSFFENHFFQIVQQLGQWDNFPSRISAASLLPLTYKHVSLEKQNTLWDLFKQLCGDDTPMVRRVCAGVLSDLAKMKCQPQQLLQLWEALLKDPIDSVKIKAIEGSQYMLKLIDDEHDLETQLQGYFALADPKEKSWRVRYTVPECLESIIDIIVKLSNXLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UUP3</accession>
    <name>A0A8S1UUP3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0500051</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8168908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UUP3"/>
    <dbReference type="OrthoDB" id="10255969at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03263">
        <property type="entry name" value="ABC_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000665">
        <property type="entry name" value="ABC transporter A family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056264">
        <property type="entry name" value="R2_ABCA1-4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF36">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY A MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23321">
        <property type="entry name" value="R1_ABCA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="272"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="318"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="355"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="388"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="456"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="539"/>
            <end position="768"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8168908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="859" mass="98667" checksum="87C3BC9086E016EC" modified="2022-10-12" version="1">MKKSYQFRALFYKNAALQSRQIGTNLCQMFTPLLCLGFVYLIQEVAFDQFKGANFSLDFPYLFNVPVIYSGLPQLLNLTCLQWYYFNSSQLGEQDKQFLGYNDGNQSVGLLRHILQRPGCSYETQDHQLAFKNVPFLQQPNSTINLDIYHQIEYLHTVPFVRGSDLQDLYLIPDGAYTFFEANEKKINMSIQVNDIRLHEYHRSNGITKIRYQNKVQPNKTNTMMLVAEGQIATIDLISRALLHYHNPNVWLVSGIEYMPLKGQDKSFLLKLINVIGAMLYPLSLSLLLPVFLYAIVLEKEERLLQMMKMNGMKMKDYWILQYVFSGLMTFLTFSLFLFTGIYLAGLALLTDTNLTLLIIVLTGWGLCQISLSFFFQVFVSKARTATIIGYLLSVWGSIIALTTNLAIYPDPYEIPIYLQLIPQIAFNRIIYIFSMACAQTGCISQFSPLTSEIQGCIISLYVNFIIFGLLGVYLHEVIPQEFGVSSEPWIFRLFKKKKIEFRDEDEYRINIQDAQEDQDSKNEREKVYKIKNFEDYPLICKDLRKVYQNNVAVKCFCIAVEQGEIFGLLGPNGAGKTSILSAITGLYECNDGEAYVGGYSIKNNMQSVQMNIGVCPQFDLLWPELTVEEHLLFYARLKGINKQNERIKVQQSMSEVKLEPYFNYYANQLSGGMKRRLSIAIALVGEPLIIFLDEPSTGLDPENRRQLWDIISQCKGKRAMVLTTHSMEEADVLCTRIGIISSGVLRCIGNSTHLKSIYGGGYHLFISSHKELYLQKNNDTHNQQYYQKMIKNFLKSILPQAILVQEFNGNFIYQVDKNQLVVSEVFQSIESKKEELRIQDWGISQATLEDVFMRVVEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UXN3</accession>
    <name>A0A8S1UXN3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0510075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000051">
        <property type="protein sequence ID" value="CAD8169491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UXN3"/>
    <dbReference type="OrthoDB" id="285125at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8169491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="129" mass="15427" checksum="2CD2C6BC159110BB" modified="2022-10-12" version="1">MYQNDEDRLAELQREIKECLDALEHITKTPYPYSNSILQLYQDLNKSLKKLFIKSAKLKEQSIIPNNEMSLIYCDNSLELQSIIYNNEVQQVCQLNHKDIEKKQLNLKSMYSFAKEHPYLDDKKKKRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UXY7</accession>
    <name>A0A8S1UXY7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0510112</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000051">
        <property type="protein sequence ID" value="CAD8169535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UXY7"/>
    <dbReference type="OrthoDB" id="674604at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF8">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="326"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="367"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="449"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="497"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8169535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="577" mass="65870" checksum="50EC41143071FC7E" modified="2022-10-12" version="1">MNCFEHNSPYTLLCWVKGCNKKFLCHQCMQKHDPQHFSMIQSLNDFDTQISQIPKSIQQNLILLSSQLNSAQTQITLNKDSLKKQIIKLSDLLKARLDVEINNFCSSAIDYTTIYYKQFENDIAAIFNTISEHSQIFTPQTGRPINEFQFTQLIQQQDKMFDIILPKLSQQVQMFVQQISNNQLIMKTENFHSILNNNISKTFQLNNLLYTNETIQTQTSKSLIQKNRIMSPLIPKIDLKKNASQLFSQKQQSDRINDQSAFYNDNIVELSGSRFLQQKSINTVLTGHTDIILCVCAFSNTSILSGSAGGTIRIFYIDNAITQTKLNDHKGDVLAIIKINDTNFSSASSDQTVRVWNYQRKVCEYILVGHEQPIKALILLQDTDQLASGSLDQTIKIWSLKRPKIKLNIKDHQKIFALCYVQSKALIVSGGEGILSVFNIINGYCRDKLEGHTGNVLCLKYLKEIVNGEFVSLISSGGSDKKIILWNLDRAQKLYILIGHQDYVTCLTFDNENNQLWSGGADQTIRCWDIDKGRQVFIFKRHTDQISCIEYIASRELIISGSWDKKIRVTSKSILVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UYD7</accession>
    <name>A0A8S1UYD7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase M14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0510091</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the peptidase M14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000051">
        <property type="protein sequence ID" value="CAD8169514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UYD7"/>
    <dbReference type="OrthoDB" id="10253041at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050821">
        <property type="entry name" value="Cytosolic_carboxypeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040626">
        <property type="entry name" value="Pepdidase_M14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756:SF11">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18027">
        <property type="entry name" value="Pepdidase_M14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M14" evidence="5">
        <location>
            <begin position="286"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="576"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="611"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="611"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="628"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8169514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="787" mass="91472" checksum="50B56380F155A1E0" modified="2022-10-12" version="1">MNSNNNYNNDSSSEDDLQVYETLTKIAPANELLPRKLTIDIDVKSLYPLKPLPQFCKTYTTFFEESSQRECLIPDIQVSDENRTLLDYYEENKQNLVYKRFDQLDLGKRTSNDRTYFASLLANFKRTQPRVFFRGEALFDQKLTPCSSEQIQFDSNFESGNLFCAFKKAENIYDLVIQNDINTRGNTQWFFFSVTGAQAGQTIQFNILNHLKTGSLFNEGLQPAVYSVKENQLVGTEWCRDGFNISYFKSIFMKEYPLSLKKKYYQLRFHYTFKHEGDKVYFAHSYPYTYTNLLEFLNTQLEDQERNQYLSRKVLCTTLGGNTCEVLTITSNSLQKRAFRKGVVFLARQHPGEPQGSYVMQGIIEFLTSNNPQAEYLRQNCIFKIFPMMNSDGVVNGNYRCGLEGSDLNRRWKKPNKYLHPSVYYAKKYVKGFSKERQILLVVDLHGHSRKQSSFVYGCAYSSQVKTIERVFALLMSKVNPFMDYSSCTFRVESAKDKTARIQLWRELKINWVYTYECSFYGQQKKHYQIKDYLNCGVSICNALSQIVKDTTKEFTNQANQPDIQQQMLEELGKMPQTDDQDIGSDSSQSEAELSDDELVQLFQPKTYSIKQKNLSKQQETNNKKKIQQPNGNQKSTIASQKASILQESPTKNQEQRPPMSSQKPKNPLYRIQAPQRNRQLDQVKQVEPPLMVDKSVQTDDWIYRQWLIQMGKLKKQQEFQSITPTRNIYKSSSQSVTRFIVKNSTLKNDRGRVDSISLMASGLQNKLISSSQQRQMMRYSGSNFMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UZI4</accession>
    <name>A0A8S1UZI4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0520148</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000052">
        <property type="protein sequence ID" value="CAD8170101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UZI4"/>
    <dbReference type="OrthoDB" id="294879at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="18"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8170101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="270" mass="31507" checksum="EF6DFACFC1513266" modified="2022-10-12" version="1">MSFCQEPKVDCQYLKSVIKNLNNRLYTLENANQNYEQKNNQVMEQITEDLVIPKLKLNKIQIGFIENNIPQKKVLVTFGNKENFRTDNCDQVGDRIDQNIFTINQPSPQRKNIIQSQLMNQQLQFLKQKDDKLTNINKQPKLKLVKQDSNLIHPIFSKKNSDQQSIGSKFYTRNNSIQSTPVKQIAYNSSLGTTPLRRKNSQKNSDLFINQLQIDDMMHNQSKFLKYFQQNYGSNKLSTTFDNSNNNLQRRNSLKSQNKITLIDAQIIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V0F1</accession>
    <name>A0A8S1V0F1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0520093</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000052">
        <property type="protein sequence ID" value="CAD8170007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V0F1"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002303">
        <property type="entry name" value="Rab_A02 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="173"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="182"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8170007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="225" mass="25390" checksum="F64462FF15400423" modified="2022-10-12" version="1">MIKEYDYLFKLVIIGNSGVGKSSLLLRFADDQFSESYLTTIGVDFRFRTLPIDGKNVKLQIWDTAGQERFRTITSAYYKGADGIVMVYDVTQGQSFDDIDKFWLHEVESYGEKNVQLLIIGNKNDLDEQKQIETSKAEEYCKSHNMLFMECSAKTADHVNNAFLELSRKLMAKKDASQPPKTTNTTSNVTQQSQSRGQTNTNTSQSNKLSANTSNQKKQKDGGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V400</accession>
    <name>A0A8S1V400_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0560085</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000056">
        <property type="protein sequence ID" value="CAD8172100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V400"/>
    <dbReference type="OrthoDB" id="10251741at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47822">
        <property type="entry name" value="CARBOHYDRATE BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47822:SF2">
        <property type="entry name" value="F-BOX AND WD-40 DOMAIN PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="189"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8172100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="362" mass="40962" checksum="658699841FAE6DF7" modified="2022-10-12" version="1">MDPKQRKDRSTSRDKKKTNKKQTQYSYGQLKFGEVQSKSKKISLKTFFGNPEFEIFCLRFDFEDQLIAAGCSDGTVKVFNIVSGKLAFNLQGSSEGASPTTAIRWRRDVANKAKSVFLSANSDGSLMMWQAQTGKQLFRTVEQGNQLLALDIRSDGDQFATAGKDFKIRVYDDEKKEIIHTFDKADYNQPGHQNRIFALKYLEETPNILLSGGWDGNLLIWDLRDHKSIGTIYGPNLSGDSLDFRNGQILTGSYRTNNQLQLWDFGTRQLIQEIQWDKNNNNSDFYVYSCQFSKINGDTILAGSSGKQELKLFDINNQYQTCGYIQDLQEGVYCVDYGNKSNKFAFGGGEGVVYICSLTNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V447</accession>
    <name>A0A8S1V447_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0530146</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V447"/>
    <dbReference type="OrthoDB" id="444540at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051581">
        <property type="entry name" value="Ca-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34524">
        <property type="entry name" value="CALCYPHOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34524:SF6">
        <property type="entry name" value="CALCYPHOSINE LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="24"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="60"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="227"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="263"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="331"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="367"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8170612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="494" mass="57649" checksum="3D17C9B7651A4FA0" modified="2022-10-12" version="1">MDWRNKLELIQNKIRGQLHARKIDDLEGVYQLMSEFDKDNSGYLDKDEFQKFLSKIGVFLTTQELRAVYDKYDSNKDGNIAYAEFVNLIRENMSERRINVVRSTFAFLDQQRQGRLLLENLYRLYQAKNHPRVRTRQKTADQVTKEFVNAISKRSKDCQSISEDEFLNYYADCNATLPSEKEEYFTDLLTSTWGVTSGADYVSPERLAQLEIILFEKIRQKTVTKDDEGKTAKKAFMYFDLENKGTIDIYQFAQALQKFGCVFSDKEIQALFNKYDSDKSGRLCYDEICGLIALMGSGNNANVNPVFQIARAPPQETLNRIRNDLIKKGQHSVIRMATIFANSDKNKNGTLNRQEFQWAMKESGFLLTKTEYDNLFRYFDKNCDDEVSFVEFISFLRKPLTQFRLDLVNQFWNRISNGQQSISIQQFKQLYDATKCQDVTLGIKTVADATKDFNDIWKDVQTVTQQQFIDYLTDISALIESEVTFEKFIRNSWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V5G4</accession>
    <name>A0A8S1V5G4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0540081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8171039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V5G4"/>
    <dbReference type="OrthoDB" id="287405at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8171039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="120" mass="14066" checksum="0056F97712ED96B8" modified="2022-10-12" version="1">MVEKSTMRWLCAICTCCWWLNYYGFLNNCVRGCQICIDVFDATVSAVWKGIFCVFMCCCFGFECVACVLCIYSCIELFNDPCQRYQAEDSWINAFWQLVYLKDLEIEGTVREKLKEIFAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VE35</accession>
    <name>A0A8S1VE35_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0610249</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VE35"/>
    <dbReference type="OrthoDB" id="118550at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8174725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="248" mass="29957" checksum="042CBA06B63C1FAC" modified="2022-10-12" version="1">MSQGEFWPKQEEIIQGDQDGLQKRKNKKRVITNPSQQTGHWSESEHQTYIDFLNMHRSVMESQDQKKTSKIFKLMSETIGTRSPSQCRSHHQKFNPFVHQIKKRQKGAGRRKKENQNKDKMNKNDENAFLQSLQIQHQLFQQQPLEYQMFQFPYFPCVPVFDNQLKEDEKLNEQQQLYFQQCMLFQQQLPQQLDEKNICCFNFNFMPQQQLHLNFNYFQQQQQQMFNNDFQALNSGDINNKENQFDEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VH53</accession>
    <name>A0A8S1VH53_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Kazal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0640040</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8175843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VH53"/>
    <dbReference type="OrthoDB" id="291956at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035895955" description="Kazal-like domain-containing protein" evidence="1">
        <location>
            <begin position="16"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="2">
        <location>
            <begin position="260"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8175843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="333" mass="37020" checksum="AFD8C3F70007A3FC" modified="2022-10-12" version="1">MNYLIVATLILFTAADKIKCPLEIDTSVSCTKENQPVCAFSLEGYQLGTFENPCFACRAEKASYYELDACIMTTKPQEPINSDSGNNSSTTSNTIYKCDIPKSDSPICPAVYKPTCGLFIPSIQCLKAPCGRSFGNECEACSDKNIESYFYGECEEIPKEDPQNPPDDSITYCTEPRPEICTDEYTDTCAFISTPCFSDSCMKPAGNYCDACTRKDVVGYVKQSCDKYKQVYEQDQNDLDDKQPDTQNDNEAQDLDSQNYAIQQKCNTKPSSCDNVVKEVCATYTCNDTTCQKEYQNECQACLESTTISYIWGKCQTLSGTILSLALLIQIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VMC4</accession>
    <name>A0A8S1VMC4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0670155</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000067">
        <property type="protein sequence ID" value="CAD8177365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VMC4"/>
    <dbReference type="OrthoDB" id="300529at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038835">
        <property type="entry name" value="Giardin_beta-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37027:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_148, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37027">
        <property type="entry name" value="KDE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="108"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="224"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="13"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8177365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="262" mass="31741" checksum="24C892166DFA5A08" modified="2022-10-12" version="1">MKYSNNPQFQSIEKVRRESPQTDRLKNLTEKWSNLKQGLDKEKNDKKELVEQHIQRIESLLNQERPKEEQKFKTLKDHLLKLQDQVYQEKNDRELFDELKQKDLRNFEEQLTQQVEDEKSNKRKLELQLLKQIEERFNSLNSNLQQNNGNCKDKTNKLLQSINLQMSELKYLLEGETQNREDSQNNLIEQIDNQINAFQDMLHVERQIRDETQKKIFNMIEDIHQRIQTELRSEKQQRESMADQLLRLLEDTCVRIDEQFNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VPW9</accession>
    <name>A0A8S1VPW9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0680040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000068">
        <property type="protein sequence ID" value="CAD8177702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VPW9"/>
    <dbReference type="OrthoDB" id="10284015at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052920">
        <property type="entry name" value="DNA-binding_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43358">
        <property type="entry name" value="ALPHA/BETA-HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43358:SF4">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="62"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8177702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="424" mass="49249" checksum="DAECBFD56E9F3B81" modified="2022-10-12" version="1">MSIQREWQKFVRPPRYNYSYFDLGPEYIQLHQSYIRKHERIITNRQNCKLEMSYFELNPKTSNCIIYCHGYNGCRIEGIKYAQIVAQYGFNFCTFDFQACGHSEGNFITFGHLEKDDITSILQELEINFQQKQFILWGRSLGATTIQLKEYPNVKGLVLDSCFTNFNKLAISIIQKQTRLPKFIIKAIIFLTKGSIEEEAGFQLNDIQVQCNSNMPTLYICSDKDSLIKAKNSLKLYKQHKGIKKLIKVDGEHNDSRPLEIIQQICSWCNDRVQLQQNNYIQYNSVPQVTRLPLELIYDSNIQSGMQSSYNRIPKNPAIKLKNQINLENSQLVFHYQKMQFINEGVEWFEIHSTILANDIALLKDNYIMNFQGKHRRIALGSSIGLLMLTQLAQRNPIGALRIGAFTFPILSYVICPEIYNSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VVK3</accession>
    <name>A0A8S1VVK3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0770080</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000077">
        <property type="protein sequence ID" value="CAD8181804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VVK3"/>
    <dbReference type="OrthoDB" id="421226at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="251"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="316"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="367"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="398"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="7">
        <location>
            <begin position="502"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="683"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="751"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="683"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="699"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="763"/>
            <end position="780"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8181804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="901" mass="107219" checksum="3807EC8751874CF0" modified="2022-10-12" version="1">MDQQESARSINISSSRYSDKFQPQLIQMNANHIQKNKLDSQDFSPVYERSNDEMAHDKQMRQTRANLKKSSNYYTASQFPQDRIQNTRGSKFIQLIKQKNQLKKFKDKLFQNAHIYPRHPNEQLTNFLEEQYIHKNQHHQKNSQQEVRFDQVNMELQKSIDNVQQYQKIPIINPTGKFYMFWQFLRLIQIMFLLWWVPFKISFDPPKTSAMNQIENLLIYLFGVDLLIKLNRGMIDQGQIVYDRLKIVKHYIIYELYEDAIYLITLTFVIGSDPITIYFFPEIIVLIQFTLNFIKLKKTINKYGEMFAIQSNFTEFVSLSQLIILIFYFAHFMACIWYYVGNMSQSSFTNSWIQQQNLQNSEVFHKYGYSFYWATATMVTVGYGDVTPQNIYEVLCTIIMIFFASVVFAFSINAIGVIFSNIDLQKQSYKRNLMLINQYMNSNEVSLQLQSRVRNYLKYYYEQQVKGNKQEINSIIEKLSYNLKQELLEDVQIRAINCVEFFIKNFQTSTIHQIACRMSIQQYTPREIIFQSNSIDENSIYIIQKGEINLIDNKTNKIIQKCSKGQCFGEFEFLTSYKRQTKAISTTFSSIYRMSRETFLDIIAENSIDFQKYCEMRDKILFNYQDLSIKCAACQGDHLINNCNYITYKPDTEFLIKKQLFNSNQERSSFNRKNLRFQRIFNNHEDHDPPNKIQHQDEQSISQDSGNRQNSSVLESEHQNILLNDETTQHIKKRISLWVTNPEQPLIMEEKKSIQKSNRHNSDSPTTKQYKKSLYNSSQDQSKRHITNQYLQISDQQQQIHQQVMNSSNNSQTKSNHQFNQLEHIQSFHSFNFELDKMETFQIYFPHNNILNVLNSYQQQQKLQKFYKINISSNKYRFIFIQKQPLPNAFKKSLYRVNSQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VYQ8</accession>
    <name>A0A8S1VYQ8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Deleted in lung and esophageal cancer protein 1 Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0780220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="843225at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008285">
        <property type="term" value="P:negative regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033304">
        <property type="entry name" value="DLEC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059041">
        <property type="entry name" value="Ig_DLEC1_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46348">
        <property type="entry name" value="DELETED IN LUNG AND ESOPHAGEAL CANCER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46348:SF1">
        <property type="entry name" value="DELETED IN LUNG AND ESOPHAGEAL CANCER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23277">
        <property type="entry name" value="Ig_Dlec1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Deleted in lung and esophageal cancer protein 1 Ig-like" evidence="1">
        <location>
            <begin position="78"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8182460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="328" mass="37838" checksum="6DD2F10A9CE57CB9" modified="2022-10-12" version="1">MQETIQLKPVTQKKNKSNFLLEMNEKDKEINNQIIKNMNKRVNYLKNPRFKINKAPVLFSYINTKQDIVVDKDQITSFRVFPKQLVFREYMLNGMYEIDLYVTNATGTLQRIKVLPPEKKEFQIAEIKYPTKEDGFIAPGMSAILKVRFNPSSLAEFDDTIAVITEDHILKVPLLARKEPPQLDLPSQLDCQSCWIGDQVETRFVVKXDGSKKSYKSLENAIALSFDERDSLLICFAPTPDREAFGTTIKTKVEEFMGKYQRKWQYKQLDASYRAIENVIEMINNSDDLDFVVFGSNGYRAQLENKTFFGSTADELLKKAKANIIMVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W7R2</accession>
    <name>A0A8S1W7R2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0770148</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000077">
        <property type="protein sequence ID" value="CAD8181906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W7R2"/>
    <dbReference type="OrthoDB" id="287041at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000184">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="2">
        <location>
            <begin position="494"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8181906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="674" mass="78321" checksum="67196A49AD385D5E" modified="2022-10-12" version="1">MFYSRSNPKLSDCQNKLNTSSYSSKSRGSFKEISPETRNITPNNQNRPLTARNEYTNPSKVKQLLNNLIQGDQNKTSTQLFGQSLFQKKQIDSRSSRGDSLLSSRISNFGKQNVKSPQNNETFLKSAFIIPDTSRDQDRKPYQNLYYQSNKQNDSNISQSKSNKNSKLLTISQIAQQCKKNSITPTRQNTINNENVNNQNNINYKANSNQKNKQQQTFIAQVCSNKKNPTTYMETFNILKQKLDNNNIQSNQTSGKKQNEKSINVSPLKSPKSKIENTKQTHIQIIDDVTQQNIYKSLLQKEQIWNDIIILIDNPNQMTLATKCTQLQKLIRIISEKHHSEYLFRIEPQILEIFLFESFACYLLVSDYLQDQINEDQNIKNLIQYIINSNLYVLQVLENVTNNSLNLNLLKQRIDLRTFNVRKVCPKGKQALQKNNFIIKSILGLMIDEITRPEIFMILSRITTNPDQYILQLKKINSQIEQIMPLDLERALAASNKDYTLVLDLDETLVHYQEFPKGGGQFLVRPYAEEFLDQLSKYYEIFIFTAALPDYANFIIDIIDKKGVVKQRLYRDKTIFKDQVYIKDLSILNRSLAKVIIVDNMPENFQLQPENGIYIQSWFGDTKDKALKDLQPLLEQIAIKKCKDVRVALNQFREQMIERVQMGIKNPYQYLQLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W8N3</accession>
    <name>A0A8S1W8N3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0850014</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W8N3"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="67"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8185297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="490" mass="57352" checksum="934CE1E20E143EF5" modified="2022-10-12" version="1">MNKKENLLRLHDRVKKDLFVQNNEEATIQKLSPQILEYVQCSSHGELMEETFQISIDQRNRKFQDDYQIGKILGEGAHSVVRQCWQISNPDETYAVKITRNGDPEIMGIMKQTFLNTLKLNNPLIVRTHKLYIDTNTECSYLVMEYLPYPSLHELLKQNILTQENKLSIIRQCLEATQYLHRMGLCHRDLKPDNILFDQDTSTIKIIDFGVSKRFITTQQRDIQVQLLWTITGNENYRAPELWNGSGYNELIDIWAIGVITYQMLCHKLPITYENRMEFNENQNQQQYLLSTEFKSLDSLSQDLLKRLLSYNPNKRISASQALVHPLLYQTYTISQLQYMKLNSKQYTKDDMLMNNKVQITAHSSQAIKYLQQSQEDHQTVTHDFKNKCFIDAIHQTDKDTSQQIRNRSIHFQQSINSLCQSYSTKPDEVKEFFDIVQKCDSHSSLDSLDDRIKASNSPPNGLDIINEEDADTPLFMNKIKQQQQEFLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WAZ0</accession>
    <name>A0A8S1WAZ0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0870127</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000087">
        <property type="protein sequence ID" value="CAD8186541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WAZ0"/>
    <dbReference type="OrthoDB" id="301912at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="345"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8186541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="775" mass="92617" checksum="37B3D126A488770F" modified="2022-10-12" version="1">MNQLNFDKLLYPIMMSIRSLRTYFKQDDENNRIILDCQNKVETFVLSIMHFILDLIVNGSNHTTISFNNNVPILQRELKNKLECIQNFVQTKDVTILKKNILKFYDLREGFSETESEDEGVSLQIKLTGKMKEFNPEIQQLRLELDQKESTIQALKQKLSYRQNNMEQMVHNHHKEVSVLKAQFALNPQPDDHSSIFNIKYFDQTQMLDPEMVVLMNEKIDDIKSQYERYVKQFKDQFQKQRLQQNYEENIKKPQIDDFTSKDLLKIALQKETNPYIFFKNIQDLKSINYIINILNNQENEYGIDYDKINNVLKTKNEDLKRIIDTRIIMEDCQAQMSAQYLVDLEKKKEEIFELNQMITQQEIEYKHQLKLYLDLIQNNYENTKQNEMIINQQQKIEQMRSENHNLSTMLINQKQHFQQLKQQNQFLMNNLKNILNILLKNKGQNNLGLDKYDLLKEKSTPIKDILNKLNNLEGYDLITLIQEVKLLLYQSESLKVVNQISERGKLNKQTQTVPMIQQFIEQKDEKKSKEKKESKLIMKHLERIRQENIKQSKLKIASTQTDSNDLNLLQDQETIYNQQTRLMSQSQKCGNQPLNMLLKQVQSSLEEKHKRFRELTSEPSFEKHNSIFDKLYTQQKFENPRWHQLKSLIEKLTDDEFLEVINTLEIYSYKQQQLTQNTTLEIPQYDISRIQQTINYKSINNKRRDSSVDQTKNVFIELDEQREQASKLFEQQRIDTYRRRIKRLVPVQPSQCLQVEEKLRPKSQIRSSQQKPSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WC50</accession>
    <name>A0A8S1WC50_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutathione S-transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0870223</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000087">
        <property type="protein sequence ID" value="CAD8186731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WC50"/>
    <dbReference type="OrthoDB" id="6507003at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000202">
        <property type="entry name" value="glutathione S-transferase 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000067">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051369">
        <property type="entry name" value="GST_Theta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43917">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43917:SF8">
        <property type="entry name" value="GH16740P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="4">
        <location>
            <begin position="87"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="GST N-terminal" evidence="3">
        <location>
            <begin position="235"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="4">
        <location>
            <begin position="322"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8186731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="468" mass="55599" checksum="3AD36ADA4CB454D0" modified="2022-10-12" version="1">MSITLYFNPYSHRCLSVKTVLLLTKSDFNEKIIDVLKGENLKQAFTNINPNQTVPAITEGFFSLFESHAIMKYICINKPDFKLYPNTLQQRALIDSYLDWHQNEFTKLLDYSKECYLQPLLIGNKVPENRVSRLVDVEEVMHFFIKTFLNNGQYNYVFDQPNFTLADIRAVCDLTSLFICNFDFEKFPVLEQYIWRMFKITDLYQSHKDYFNLIQKQKYKNQFIQSILTKQLKQEQITLYFHPLSSPSRAVRSLLLLAQIDFDEKVIDILKSENKSDQYTKINPNQTVPSIKQGTFTLFESHAILKYICEQYRLFDFYPQENLRLKALIDNYLDFHLNEMKQITDYVLNSQKILNEQELKEKQLNIDKLLQFFVKIFLNEGKYKYIYNHKTISIADLSAVNEILFLVMINYDFNQVPQIQKYITNIIDNEYVKQSNKEYLSTISSNQHNNFNQFNKQIITTQKKRGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WCJ7</accession>
    <name>A0A8S1WCJ7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0870115</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000087">
        <property type="protein sequence ID" value="CAD8186517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WCJ7"/>
    <dbReference type="OrthoDB" id="284523at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8186517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="60" mass="7417" checksum="95C1A8120F742A5F" modified="2022-10-12" version="1">MNSEIIEVCQHQKDNILVKMKNGEKKWFKFDEVLKQQPKMLAQWLEKQVFFREVEEDKLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WFI2</accession>
    <name>A0A8S1WFI2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0890075</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000089">
        <property type="protein sequence ID" value="CAD8187435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WFI2"/>
    <dbReference type="OrthoDB" id="288905at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF263">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="33"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8187435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="564" mass="65779" checksum="2CDA331554BEB48C" modified="2022-10-12" version="1">MGTCQYNNCKSYIDISTLNTTVNIIDQLNNDFMVVHEDYEHPLYGQVNILEYKEPKQYDFKLVHKLITFTEQEDFQYALQYYQAKFYGFNHPNLLIIHAMQYKQIDQFFTTEYKLSLFLDYYETTLAQELIYRKKIYFEETEILFFLDSLIGSQAFLQSKGYALPSLKFDKVYLSNLLNGAIGLKVQSPLFHNQQDVEFNELFHKIINGEEVRLHEYPYITPEQWLMIQNKQLEPYNEFKSNVFILGLMTLELCSAKQSISLYQDYMIDPNTLIEQINKVNDRYGEALPLILEAMLHYDPKDRRDFLQLNELLDSSQLKGKRLAQIWTSDDYQYSLTEKKVHFSTKQHSLVHPEVQSQLSNQSLLQKGSHLSIRQVISKNSKESKASSSFIQKKESYSPQIQKKNLFKKFETEQASKGGSKKLITQDGFGVETTPNGLTYEGFFNAGKKQGMGKLINDENEIIYEGYFHENQFHRTGILKNQNAQSCQEAFNYKDFNQLGEKWARYDGDFNFGQQDGYGQLVLTNAERYTGLFKNGKVHGAGVFQTLDGKQIKGVWINNILQEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WFS1</accession>
    <name>A0A8S1WFS1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Starch synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0920168</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Amyloplast</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000092">
        <property type="protein sequence ID" value="CAD8188918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10263625at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00551">
        <property type="entry name" value="AmyAc_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03791">
        <property type="entry name" value="GT5_Glycogen_synthase_DULL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013534">
        <property type="entry name" value="Starch_synth_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45825:SF11">
        <property type="entry name" value="ALPHA AMYLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45825">
        <property type="entry name" value="GRANULE-BOUND STARCH SYNTHASE 1, CHLOROPLASTIC/AMYLOPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08323">
        <property type="entry name" value="Glyco_transf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0035">Amyloplast</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Starch synthase catalytic" evidence="6">
        <location>
            <begin position="1213"/>
            <end position="1453"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="5">
        <location>
            <begin position="1505"/>
            <end position="1672"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-activated protein kinase glycogen-binding" evidence="7">
        <location>
            <begin position="1770"/>
            <end position="1839"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8188918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1840" mass="213143" checksum="BC5367BAE5DD0610" modified="2022-10-12" version="1">MFQLYENAENSTYSFEFKLDLTLLNVLRSFTHKDHEFQEGYLESIYALLYYYQGKPESELFSEYCAQKGNHTIEALIFQVHYYIASLANLNQLVGFLKVDENKVIPLSRSDINKTNILSVHSITDRVGSINSTILVSKSQTVKPEYLDLRVKICTCQNKEEGVFSIDYNVLSLAIQAKGKLDPLVGSHLANKLNKMFKLELYHILLYFADKKGQVTENYDERCMIIVNGVSNFSFHKKQYTDLRDKFIRLSYGNVEKNVDEITKLLMDLDQDVGKSIIARDTKSNHYLEKLLVGLLGNPLQLIRDQAVILLNILYDGVDWQKRVPFKPKITKVGSKFDIQYLFEYDSSYPLSSIVILLKSYIFDSGCKQNIVSWHKPKLSEYSSQGDKKYMAFKMDFGRFKRCGFYDWKLIRMTNSGKLQSIQKIMNLDELKSNVEITSLQMMEAKPIQGRFIVHPRDSKNLQIHEIYVDLLDAKPDNQGKFTKRGNFRKVREKIPDLVQQGINCVYLMGTLERDNGLMIDQDKQQKLFKRPEVSPLAITCRLTPNQMLGGKEEFMQLNKVAQQNKLRIIVDMVTRISSARPHRKYRKDLVYRLDEQGRQVAMFGTDGRSLHFEDTIILNNRKKRVWDVLLEEMIELTTQYNIQGVHLDNGSSWPQVFSLDLDEMYRKDSDGNQAYSNKQIFNGEICLQNEDCGYWGTSVKNLYPNPFLVKICKSLWSKFPNFLIVAEAWGAMGEQEEREINLIQSGVIPRLFKLPIAIASIFGENLKKDGTMVKMERKNVNAIKKWIEYTAKQYPSGSIIVQSTTYNSWPYPALIYKRGTWAAVDLFFTLQDIPMTFMGEEEGFAFREKTTNVFNYVTKHESSEIKLQDPTQQKTPQRSLKRAESYVQVNEDYGFGYNTLTHQVQLQIAQSPARPNNNGQPVRVQSGSSLSQMDISNIATAQENFRREVGPQFGFDLQQIGKHYVHIRQMRKQHKVLRNGQLVSLSAEHMYGWHTHVLAFARYSKDEMALIAINFNDGEIDMFMNLRNLRYYFPNSERSNIVVRLRNWSYPELENEEVSYFYIGNFLTSRLEVHLKNFQSQIWGVEILGNSQEVQQKAQKSALIRLQQKMQSKIPLTIYGNDAANKLTLLTDNLNSLQQFINGFSHYYTQVIKPNNFDLNRLISSMKDFQSNKVRLCKLFAFFQSILGTQDKNLPFYDTLQQLINLNKLGPICFCTPELGKWTTTGGLGVMVDELTQELAKMNEEVILITPYYHRNKKGDTGYILSDGFKHIRNIEIWLQGEKVILGVFEGVFNGVRLFWLHNELYFPSAYAGEDASYVMKQLTVYAKGCLELLCQLKLIPSLIVTNDWFCGLIPGYLRIRRYGEAFAGTKSFHIVHNLDPLYEGRLYPKPNEGTLDYIHELPNDFLIDPFWQNLVINPSRCPLITCDNWGTVSQSYKYELLESSPLASVLRKHPLPFAFPNGIRREQRFKVIMDKTNNDHLKAKEQLQKKYFGCSQLENHIVVLGFVGRVTKQKGVHLILEVAEELIQRSQGTVQILVGGPADMKEEYSAFCAQNMIRLKSIYPRNFWADPSAFFMDGTLVNVGCDFGLMPSLFEPGGIVQHEFFIGSTPVIAFKTGGLRDTVHEYDQKQQKGSGFIFDAYNRGNFLYAIDRALQLYKNQEQYAQLRKNAFDAAIDVADVSRAWAQEYYRLFDKNFIDKELVQQMAGQIQQDYKELKEQELFTKEEGRVFSNSDKTLKNFERILAHAKRSNLRLHQFVYRSTRLLQPRQVAVSGSFDEWKEKHKLKFDHFSKVWNVTLKLLPGEYYYKFYVDGEWICTDDDLKDNDIYGNINNFVIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WI64</accession>
    <name>A0A8S1WI64_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0900084</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8187869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WI64"/>
    <dbReference type="OrthoDB" id="296361at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8187869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="114" mass="13184" checksum="B1C2845B868E24B4" modified="2022-10-12" version="1">MSIETEVLYALYSLYQKNLISYEQKGMIKDMLIQNDFVLRASIANCDTQFLLQYRILDLLKDQESPRNSTCISPLKRMTSCSTTQSRQRIKSANQNPNQKKTISRQRSNSLTAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WI97</accession>
    <name>A0A8S1WI97_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SP-RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0910084</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000091">
        <property type="protein sequence ID" value="CAD8188327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WI97"/>
    <dbReference type="OrthoDB" id="28127at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16650">
        <property type="entry name" value="SP-RING_PIAS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782:SF4">
        <property type="entry name" value="TONALLI, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782">
        <property type="entry name" value="ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02891">
        <property type="entry name" value="zf-MIZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51044">
        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SP-RING-type" evidence="6">
        <location>
            <begin position="382"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="515"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="532"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8188327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="542" mass="64311" checksum="787AD211AF1E9CE9" modified="2022-10-12" version="1">MKIMKSQQLQKDLEAFKKQLKQTLPTIINRVTKDIGQILYFEKLFKDQKANSVQELYFQSLLRLDPHIARQQPQLDEPIQNINEQIHLYFEQQTEKLQEMVLLEKELKMMRELEELQVSQESTQIQNASIELEQQKGMEIEKQNIEEVCNTIKRKYWRQVQTPNCDQCFCGNQLHYKQIVKCHLCEKHFHVNCLDKSYDERYVKNFTCPRCTLYHMDQFCEVISIIIEPFSFKKTGLTSTKTIKFKSDTNKIDIRCIRMDCPLSAEEITWPDLGELHINNKKVAEFIPLAQQSCQHKRKDEKLIFTIPQNEECTLMMKEIIPGMEQKRKYRIQGEQLHYVAVYKTKQYNAKQLIEKIIVSSENWMSVEQAQDFIILQMNYISSTGIKQIKQTISLLCCLCSTLMITPVRGIYCNHIQCFSLENYLMMLEFSNPRKWRCPICKAKLFKLQIDALQYTILQTIRQYNLQDKYTEISFDHMGNILDDLIQKYLDFNILPEHAKTSRNRILQLETLSNQENENEIETNESEQQNNRPFNPNSIVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WJH0</accession>
    <name>A0A8S1WJH0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0910080</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000091">
        <property type="protein sequence ID" value="CAD8188319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WJH0"/>
    <dbReference type="OrthoDB" id="10020333at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045216">
        <property type="entry name" value="CK2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054:SF0">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="39"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8188319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="401" mass="46994" checksum="8FD066AFE7D8811E" modified="2022-10-12" version="1">MKKNNKKKSDPKVKVNPCSNDRYYMDQSIDEEVYIKNNYKVVRELGRGGYGVVYKAVGLNQGQIDKNKKYAIKVNFSTVSPELIFAEIGFLKLIYGKENMPQLVNLFLIDQKIYIVIEYFTYKPFITFFASFDMMEIRRYLYELLKALNVLKLNGIYHRDVKPGNFLYNPKTGKGILIDYGLSEIDRSFVAKLIEKEKELSKKHPQSDEVQEIRRKINLYNEIQKTIDQIGNNKIGTESFMPLESILHYHDQSYEVDMWAVGVIFLQFLTKKYNLFSNVRMINKPVVTKNFFYVNFILELASLFGSDQVKQICEQFDYDLKLPSVVATKPVQWKSIIHIDGFDSDAEDLLTRLLCLNPKQRIKVEDGLKHPFFKPLLDQEKITQTNFQDDQEIKQQKLQEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WL69</accession>
    <name>A0A8S1WL69_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0930173</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000093">
        <property type="protein sequence ID" value="CAD8189361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WL69"/>
    <dbReference type="OrthoDB" id="308556at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8189361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="178" mass="21047" checksum="64FD1C0F052AFE54" modified="2022-10-12" version="1">MNIDHTDWVCSLSLNEQQNKLISCSKDSQILIIEWSKLDKQWSVTQKIKVDQYGYRLCFINDNLFTFQPYGKDQMYIYEMDTNNKQYNKIQEIELKYDSSGCDILFPQQYIKSKCLLVNKNGKNVNLMRKKQNGDFIIQQSIQFSSNTLYGQLSDDGEYLITWDDGSKEIQIRKCKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WNJ7</accession>
    <name>A0A8S1WNJ7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0950104</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000095">
        <property type="protein sequence ID" value="CAD8190045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WNJ7"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001172">
        <property type="entry name" value="Cystic fibrosis transmembrane conductance regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000330">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF330">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C MEMBER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="124"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="39"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="327"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8190045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="576" mass="66492" checksum="9C15CF4D6078B790" modified="2022-10-12" version="1">MQGSMSLIDFWLKSELKGNEDTWLHQITMYFNNSFTDTLLYLTLFALLVTFVRAALYVSTALRSAWVLFVKLNKVLMQSVMKFYDKTNAGRIINRIQDDTQMIDDDLSWTFHCFLENVSRVTGLSIGLVILEPIFVLILIGVVVLYYQVSKIYIKSNREIKRLKSVNQGSLLQTVNETLTGIKIIRAFDKNSLFCQTFQNKLQNWVMTDQCGERSKLWFGVRLNLMSNLILIFVSSFVILTIMFDFSDDYSVQALAITYSMVVLDSFYDLFSLYLRMESGIVSVERVKQYFSNETENIDKVQILPKIDFDIWKDVKEFTEFRIENAVTFKNIYLTYENVSAEAKFALKNFSLTIKKGQKIAFVGRTGSGKTSILNILFGLYHHQYGQIFINGMDSSKLTLRELRSQLSVVPQFGFLYNASVKDNLDPQNKTTKDVIEKLFIDTGFQLRGINNTNNNSCNADFIISQNGSNLSNGEKQVLNFLRIVLMNKEIICLDEATSNMDPKTDQLLHDLLFKFAENRTLLVITHRLENIKIYDKIVVMEQGEIVEQGEYQELIKIEGGFFNKLMSHHQKQIKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WPN9</accession>
    <name>A0A8S1WPN9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0970018</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000097">
        <property type="protein sequence ID" value="CAD8190601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WPN9"/>
    <dbReference type="OrthoDB" id="297450at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="114"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="13"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="26"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8190601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="327" mass="39125" checksum="F7B0982D85D200EE" modified="2022-10-12" version="1">MDPSSSQTYIKQHPQDNSKSNENKDGSQTQIKSQTNFRNGQEKNDIGQFKDSELQTIEFIADNIKYQLDPRYMLWSTELLKQDKPQDQNAYNYQQENQSIKKKSHKFMDLKKLYEHNKSTIEGLNKELNDLELARQKTLQRLYEVRQEKERMRKIFKVERTKTENLKGCKERLDKLKKELAQQLEYFTDANFQKRTLISKHYVVYDWIIGFCDQECPNETISIINSFPDSYQKGLVNLSTNQWIKDNIAHKGIWIYYMNNTMCEYKNGNCDQYFQVPQKYNIPKVNFFESRMIVIQIGCVILVIFYIMAFFDLKKRFWDTKKKVKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WQ46</accession>
    <name>A0A8S1WQ46_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1000027</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000100">
        <property type="protein sequence ID" value="CAD8191823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WQ46"/>
    <dbReference type="OrthoDB" id="127285at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008833">
        <property type="entry name" value="Surf2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34348">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34348:SF1">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05477">
        <property type="entry name" value="SURF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8191823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="231" mass="27401" checksum="1A00672E132963A0" modified="2022-10-12" version="1">MNKNKESGKQVQNNKVNDSNSNKNKELQKLVQNNKHFEFLSSGKIKCILTHHEILPTLQEFNNYLNGRSYKNALENDIEFTQFEPYIVQHKTDKNKLYCNLTRQNISKKKSVVLKHVNGKRYKYYLSKYLEEQAKEEQQNQRDNQEDEDDIQNELEELNNLINEEVEEEQPQKQKSGILKGGKKINKKKVKPQQEEQIEIEQTVQTKQKKQQQDGKLQKKLKNKQQKAQVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WS92</accession>
    <name>A0A8S1WS92_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S ribosomal protein L13a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0980085</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000098">
        <property type="protein sequence ID" value="CAD8191109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WS92"/>
    <dbReference type="OrthoDB" id="1882297at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1180.10:FF:000009">
        <property type="entry name" value="60S ribosomal protein L13a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3250:FF:000001">
        <property type="entry name" value="60S ribosomal protein L13a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_uL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_uL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005755">
        <property type="entry name" value="Ribosomal_uL13_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01077">
        <property type="entry name" value="L13_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8191109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="198" mass="23109" checksum="00717FC46B38D088" modified="2022-10-12" version="1">MFEKEIVIDGKGHLLGRLASYIAKELQRGQRIVVVRSELIQQSGSLFRNRVIFEEYLNKRMAFNPRRGYKHYRTPSRCFWKVVRGMLQYKSKRGAAALERLKIFEGVPPPYDTRKRQVVPDALKLIRLKNHRPFCTLGDLCASVGWNSQAVVNRLEEKRKQRGTAYYKRKIARENLRRKAIGAKELTSINAELEKLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WU63</accession>
    <name>A0A8S1WU63_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF423 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1020185</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000102">
        <property type="protein sequence ID" value="CAD8192822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WU63"/>
    <dbReference type="OrthoDB" id="291190at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006696">
        <property type="entry name" value="DUF423"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43461">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43461:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04241">
        <property type="entry name" value="DUF423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8192822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="112" mass="12224" checksum="7E72CD6D1157AE73" modified="2022-10-12" version="1">MQKNWGFKTGCLTIASGIIMGAAGGHKPWPIEQKLIFDRGLMFHFLNGIGMIISSVQGVNILPFSLFGTGVLLFCGPLYHKSFTGKKTLSGYLPPFGGFAMIFGWILLALRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X397</accession>
    <name>A0A8S1X397_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1080092</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000108">
        <property type="protein sequence ID" value="CAD8195035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X397"/>
    <dbReference type="OrthoDB" id="299817at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035738376" evidence="1">
        <location>
            <begin position="18"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8195035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="426" mass="47287" checksum="D2DF26B6F3D04E95" modified="2022-10-12" version="1">MIYFFVLLAVVSSVYVGEQVCTTFNADATLCNASGYCRHDGTICVNLPCYMVNEVAACRSGPSGSGIPTGALSSKCDSLEQFSLQYDNVCVDKGDGKLNFAFVRFSKTTDGSALQTTGTTPTGVGALAATEANMFALYTINPWKSPEASQSAATLQTELEAILDRYILFYQVLIETTNSHPFYLERTAYQALQLIRDQVGFDYTMRRAILPKIWQVAEIALFRIAKFSPAYYQTNYYFLNFASCPYSRRELKINGQLHTVRIDWTDYLYTVNGYMQVYSYAAEQFGIRNALSDIIALRPTTSVATANAINLDTGFTAFYLEWTWTDTNLVVVEKDIKLYQFDESAATATNVVKQITTTITCTTATKTCTTGLITTPITSATTRINYYFSYGPGKCVDKTEVQCKLSIEQGTQCNYNAGLPTDYKCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X5S1</accession>
    <name>A0A8S1X5S1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1120037</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000112">
        <property type="protein sequence ID" value="CAD8196260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X5S1"/>
    <dbReference type="OrthoDB" id="10565417at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8196260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="128" mass="15330" checksum="B7ACABAC9AC31464" modified="2022-10-12" version="1">MNGRIIKQQKIILVMFMDQVQTNNKIEWYLVVMINQYQQWNNKDVIKNRSQYKRLKFNNLQIEQVLLIIICSHYHKNKVYEMNSFNQQFTKTRDVDAKCGSFENCPFPQQYTNSKCILVSKNGQNISI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X997</accession>
    <name>A0A8S1X997_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1150134</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000115">
        <property type="protein sequence ID" value="CAD8197494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X997"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000003">
        <property type="entry name" value="Calmodulin A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039647">
        <property type="entry name" value="EF_hand_pair_protein_CML-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10891">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="121"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="157"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="76"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8197494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="482" mass="55902" checksum="8A7FC6D0A263FD65" modified="2022-10-12" version="1">MIIQQNEEQDIFQRQESNDTDQKLKSSYVSNEFNEVGNNSPQNSVDIKQIQAPQINQMPKVMVNASSYQSIPMSSQFRYQDDQQSQKMKTGPSDQSKITPGYTKYMIEESNQQYDYVRGMFTEDEIKEAFNFLDMNKDGGITSEDLSFFLDFIGEKATSEEIEEMIRMCDKDGSGEVKFEDFKNLAGGWSLPTLGQAHPPTKELVEKRTQINQTMMEKEVQEVARKGKITPQMVEQIRKNELTSPRFGQDEQSKLMFQSINNIASQKKQRQVIGQNLKVIRSDEGQITQQTKQKSSLSQREIMLQRKNNAIRFVREKKIDQAYILDIFEKINKLDIMESCTYEDFIHYLDILDNHQSKLLFNSMNDNPVREEYVKSSDNVIVDQIVNLKNTLLTLLGQTNCRKHDKLEIAYLLQDSNQQGYIFLDDLISLLVNLYCISEVAIIEQKILKMTAKKGLKNRKDTIKKDIYISMLNEYGQVFEPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XAF2</accession>
    <name>A0A8S1XAF2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1160145</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000116">
        <property type="protein sequence ID" value="CAD8197852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XAF2"/>
    <dbReference type="OrthoDB" id="306452at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8197852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="102" mass="11909" checksum="E571BD965AD45229" modified="2022-10-12" version="1">MSNQQTQSYTSSIIYTPIPLKLEFSASRSSRQSSNSNILEESTASSFIYSRSPRKILLAKFEQENQERPENPMIRDEKFKELQNLFLPFDSESYITEVELFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XB53</accession>
    <name>A0A8S1XB53_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T1180049</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000118">
        <property type="protein sequence ID" value="CAD8198280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="417789at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18577">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18578">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000240">
        <property type="entry name" value="ABC transporter B family member 20"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000194">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF27">
        <property type="entry name" value="ATP-DEPENDENT TRANSLOCASE ABCB1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="43"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="87"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="304"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="734"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="804"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="873"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="985"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="46"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="372"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="735"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="1086"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="614"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="681"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1217"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="684"/>
            <end position="695"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8198280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1340" mass="148488" checksum="35B0283B0DDA6949" modified="2022-10-12" version="1">MKTDGSAENQALTNGNGAVSQPQQEPMVPYFELLRYASPKDKILMFIGGFAAFCNGAAFPSFSIIFGDMTDSFSEAGDEMVRQAGLNAIYFIIVAVGTMLMSFTMFATWMITGENQSIEFRKRYFSAILRQEIGWFDTINPNELNSKVANETFAVQGAIGEKVPTFIMTFSMSFFGFLIGYIYGWQLALVVTATLPAISIATAIFAIIIQTSENATQKAYSDAGALAEQAINAIKTVKMLDGEDFEVEKYKKHLIEATATTISYQFGVGLAFGFLWAAMLWAYALGFWYGAKLISDQTLNHNMGTVYTVGDVMIIFFAILTGGFSLGQAGPCVQNFAKGRQAAVKMFAILDRNPKIVNPLNPLKLTSFNGTILLRNIQFSYPNRPDQKILKGLTLNIPAGKKVALVGESGCGKSTVMQLIERFYDCEEGEVLFGGADGINVKDLDLIDLRSRIGLVGQEPVLFATSIRENLLYGKTDATESEMIDALKKANAWDFVSKMDKGLETYVGIGGGQLSGGQKQRIAIARAILKRPQILLLDEATSALDRTNERLIQETLDEVSKGITTIVIAHRLSTIQNADLIYVIDKGVVVEKGTHQELMNMHGKYEILAKNQIQAQHQDNDSGSSSSGPSQKNLQDQQIGSQRSLKLKMNMVDQQNIAIAVKQEIDRFKELEVPELVKKVSGQGHHHHHHHHHHVKNETDLETQPLPKKEEPKKDVDAQMGRLFTYNSDEKPQFFIGIIAALANGCTFPVFSLFLAEMITVLVESNPSFADYKCTITYDNPTPELCQLLKDDLKDEVKTKADRLALWFFLIGVAAQVLWTFQMYFLAYVGEKLTCKLRLDTYRKLLRMPIPYFDIPKNNAGTLTSRLSVDCKLINGLTSSILGINISNVGALICGLVISFVASWQMTLIMLGLAPLSYVGGILQAKFLQGFSDLTDEAYKDSGNLIMEAVTNIRTVVSFGNEQIILGIYSKKVQLPLMKAKERGIYAGLAFGFSQMQMFIINAIVFYVGAILCRDEVITIEGMFKSILAITFATMSAGNNAAFAGDIGAAKNASRNIFEILDSEDEFQREERLKKQKITKPVQGDIHFNNLTFKYVGRDKNVFENLSLTVKQGQKVAFVGPSGCGKSTLMQMLMRFYEPDQGIITINGIDITDYDIRYIRRQFGIVSQEPVLFNGTIKENIQYNLPAITNEQIENAAKKANAYDFIIKNQFEETQVEQKGNEKQRGQGFDRQVGPKGAQISGGQKQRIAIARAILRDSNLLLLDEATSALDAASEQLVQDSLNKLMEGKTTVAIAHRISTIKDSDVIYVFEDGKIVEEGNYQTLVGLKGSFYRLEQGIAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XC54</accession>
    <name>A0A8S1XC54_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1190107</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000119">
        <property type="protein sequence ID" value="CAD8198650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XC54"/>
    <dbReference type="OrthoDB" id="294266at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8198650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="334" mass="40641" checksum="6B47963A0619D40A" modified="2022-10-12" version="1">MIQKLREIFKQDSEFIRFLKRSSLSKDNQVYDQEDIEFLFQYMIKERNLRDFFEFYSNFEMQTYNTKMKMLIITHQFIHSNEEFSKLFTTLRLKSFCLLKHNHRNYETWLLFNIQIPFLSYLQKLAISFKNIKSYKTCIFRKQQYSNIMIDSFKIINIVNMGLSLVPNLKTALINYPYDILLQRLTFNIYQELQGYQKNLINSMIIILIDKSKNQNVELYELLREIKVIEQKMIFYHSFRNLYDQKESLLAPLQIQKYLQNYKSPIQEYTCKVEAKKEYLQRSSRTRQSTNGETITPKSQRMLNYFEHQYSSQKYLKSEEIVIQEESEIQIDQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XCG0</accession>
    <name>A0A8S1XCG0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1190162</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000119">
        <property type="protein sequence ID" value="CAD8198760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XCG0"/>
    <dbReference type="OrthoDB" id="300221at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46818:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_125, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46818">
        <property type="entry name" value="DOMAIN-CONTAINING PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="114"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="719"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="719"/>
            <end position="733"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8198760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="742" mass="86756" checksum="EC09C65BE891C833" modified="2022-10-12" version="1">MFQDRYQYLLVSPNEIANLSIDSNYIKKGSLRRIFKNVQFDTFENQQLKEFKEYLDSIPVEYLKEVQNDSVLLRFLYAHKFVKEDTVNYFVDHLNWLNNPETMNLEDIPQYSKLVQIIGRDEMLRPVLHISLSLPLDDLFIKAITNKLIIMEDYMFVPGKVESWIMIVDISQTNPNIKPEKIQQAIKHFVTNFPMSLEQIYFLEVNLILKQIGDNILKLDEIKKLNFEDKITITSKVKYEKEKIQFLSPEYVHLFENDTQLNHSLQQNQQNPFSILQQQPSQSSLPFAQSPNQIIRNQPNQSNQMAQQPSQLILNQPQPTLYGHPSKLSNDGELIGPLYPMIVYASAQPQPYVPEYHHKEFQIQPATQVDPHHMMNNNYYNYQNNIPNQQNFVKTQYGQQQQQYKGISNPDYFVRTKYDDIIDQYNWNKYGITPQLPSVEPEKIINDVTMHISSPLQQYTPLTPTLQGIQQTVKKNDLNLNHDIYSTYNNHNNHFEDPLIINKDHFPQISEHPSLTHYDHPPDHFNKYTNIDLPIDPIPPFHQSIKKYDIDPQPYQPYQTYQPKQNIDSYPPIHHQIEKKYDFGPIPNRPSEHSFPNSQIIYDKPFIPDRSSIDTPITTMNYTQPKYQSYVPQPLSNNPINTLPSNLQTIPLSSIPQNSGYSPYYPQQPFSPIPSSTNLYTAPNYPIPITKPQQSSLEQPKIVEQDGIKFQDETMVVGDNDHRQTKNSNRDPALDQQQCAIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XEX4</accession>
    <name>A0A8S1XEX4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1220061</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000122">
        <property type="protein sequence ID" value="CAD8199615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XEX4"/>
    <dbReference type="OrthoDB" id="298503at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="469"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="526"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8199615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="548" mass="65073" checksum="7BEBCED6E6E53134" modified="2022-10-12" version="1">MDQIPNNNQNQGLNRSDTQAIPKNQTGRIIYQTYFCLLGTALLILSIMSYYFYSDAFDNYKYIIENWKQQPIQSIRLSFDKCNNEEINLINYRWPGTVDGCDCRYSFRLLSIKTNNHHTWFPRNKIYNNHACNITQRAWGCISIYAKDAINFNRFPTQNQKEGFKLCAILEKDNSFYSHLPELDECPKGQIKCGNEVNYFYCTSQSECPIFEIGFKDVTLIDNSQEQTEKGLTLLYNRKSEYKLPLVEVRVSEGEGICMKNHLRGLSNGRVEYPLMRENKTECQIDKRFIKLKSSNEVEFYESNNQLKIQDQLRGYEISKQYQWGLYARGYIEWKMSCRGEMFDNFIDQEQILEEILYTQKYLMIVSIIFFIIISVIWSYIQAHTLQGGDWPCIKGRGTEESNTIFYLEILTKIILQSLQAFIIIMSFSRTKEESNFLESVISRNCSDEYVQIQLEYLRDMLSENIYRFNWGCLILFCITTVLDFCVVSYLIISFIRDRKDKENRYEQRDEIKQNYELSYMQQEMGNQNQQQQQQQQQQFNNQSPYFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XHV9</accession>
    <name>A0A8S1XHV9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1240152</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000124">
        <property type="protein sequence ID" value="CAD8200459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XHV9"/>
    <dbReference type="OrthoDB" id="445556at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056453">
        <property type="entry name" value="HTH_DNAJC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052594">
        <property type="entry name" value="J_domain-containing_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44144">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44144:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23302">
        <property type="entry name" value="HTH_DNAJC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="9"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8200459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="224" mass="27407" checksum="1D4C2B9633316CEF" modified="2022-10-12" version="1">MQQREEQFTLYKLLNVEPKATQNEIKKSYRQLALQLHPDKNQEDADAKEKFQKISEAYQILSNEEKRKMYDETGMIEGMDEFKNAYEFYRNLYPKISKEDIDKYEIKYRFSKEEEKDLIEFYNKNCGNVKCLLENIILSRNEDIPRFLEFYDKMIQQKKITDYKIYQTSRNKIKTLKEDKKAQQIDMDQLTKQIRQRPKNTFDQFIQQMEQKYHKPKKVKTQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XKY6</accession>
    <name>A0A8S1XKY6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TtsA-like Glycoside hydrolase family 108 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1290022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000129">
        <property type="protein sequence ID" value="CAD8201781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XKY6"/>
    <dbReference type="OrthoDB" id="311757at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008565">
        <property type="entry name" value="TtsA-like_GH18_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05838">
        <property type="entry name" value="Glyco_hydro_108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TtsA-like Glycoside hydrolase family 108" evidence="1">
        <location>
            <begin position="9"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8201781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="253" mass="29922" checksum="DF3DF7D3DFC11003" modified="2022-10-12" version="1">MSVFDVILKLILEHEGGKCNRVHDSGGKTNRGITQSTYDYECGQMMENKKVKPEDRFQYKDVYDLTDQEVYKFYKYLFNKCSANQINNPETCYVFFDAVINHGQGGAIKCLQHVCNLKEDGIFGPNTEAMANSLNQKEIQKKMLIYRENQYKKSKNYEYNKNGWNNRLNKIRQQIEKGIMPNLQQNYNNYQFVQQQQVVNKPKQDPVKYLHEAIKMQQIPDLTQFEIPIQINFNQQVPDAFPLFNQYKLQRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XWY6</accession>
    <name>A0A8S1XWY6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1430008</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XWY6"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="96"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8206056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="388" mass="45452" checksum="C4BC1243A3C4AB26" modified="2022-10-12" version="1">MQFICKRKRKLFNNIYKIGIKTHDIIIIDGTNIAKYIIPFYSEIEVNWNLDNNKLIGFEFLYNKKLIQFEMKPEDCQVFQDTVKSQIFFSNLYKFYKPIYEIGKGSYSNVLKVVSYQNKNMYACKCIEPNDNFSEQDIMNEIQIHSLLNHQNIVRLLGISKTQRHFYLLMELPNGDTLKNYMCKKAKLSEEEIIGILKQLLQAVDHLHSNNIIHRDIKPENIILQHTDDQTLIKLIDFGLAANLNDPHIQYKVCGTSGYVAPEVINNDGLTPYDTKCDIFSCGVILYQLLTHKYLFEGDTKSEIYQNNKLYKKVDYNFGDIHYYFHNLLSQMLEDNPEHRITAKEALIILDHISIQIDEIPQDENIQRLPVMMSIKPKSDPNLMNIDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XXB1</accession>
    <name>A0A8S1XXB1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Trafficking protein particle complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T1420071</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="7">Part of the multisubunit TRAPP (transport protein particle) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Golgi apparatus</location>
            <location evidence="7">cis-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TRAPP small subunits family. BET3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000142">
        <property type="protein sequence ID" value="CAD8205930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XXB1"/>
    <dbReference type="OrthoDB" id="282549at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990070">
        <property type="term" value="C:TRAPPI protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990071">
        <property type="term" value="C:TRAPPII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990072">
        <property type="term" value="C:TRAPPIII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14943">
        <property type="entry name" value="TRAPPC5_Trs31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.20">
        <property type="entry name" value="Trafficking protein particle complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016696">
        <property type="entry name" value="TRAPP-I_su5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007194">
        <property type="entry name" value="TRAPP_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20902">
        <property type="entry name" value="41-2 PROTEIN ANTIGEN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20902:SF0">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04051">
        <property type="entry name" value="TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017479">
        <property type="entry name" value="TRAPP_I_complex_Trs31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="6 7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR017479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8205930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="180" mass="21435" checksum="B8B84F563290AD54" modified="2022-10-12" version="1">MKKFQFFDKNIRQQRPELNFCTFNYVFAEYICYLKRKHGNDYNEMNSKMEQLGVSVGIRLYEVVSLRERNKRETKLVEQLRFIQGIFWKHLFGRQAESIEKLKDRPNDYLIRDENPLLLKYISEEGHISPAQFMCGILKGVLNASGFTCQVSYQFKTDERGVSYYPHTVFILSFEDARDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XY49</accession>
    <name>A0A8S1XY49_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1440120</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000144">
        <property type="protein sequence ID" value="CAD8206611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XY49"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8206611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="63" mass="7677" checksum="DEA4935EF6C18BEC" modified="2022-10-12" version="1">MNNNKLNNSFPENLCNKRQNCKIRYFNDNLKNQKKLPEILKKSKQKQVSVLRQFQKQINIQRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XYS8</accession>
    <name>A0A8S1XYS8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1410052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000141">
        <property type="protein sequence ID" value="CAD8205588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XYS8"/>
    <dbReference type="OrthoDB" id="6275292at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051291">
        <property type="entry name" value="CIMAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580">
        <property type="entry name" value="SHIPPO-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580:SF60">
        <property type="entry name" value="SPERM-TAIL PG-RICH REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8205588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="363" mass="42235" checksum="49EBD57DB36B38F8" modified="2022-10-12" version="1">MLDIHFGDEILPIAQIVASSQPYVNKQINDMRLSANSNRSAMTFKLHSNRRLRINSQEQIWPLPQSSNSYTFQRQNSQRSKQSVGPGSYEIQSPAHLQQNRPSISFTKSNRRQFEKLQKPGPGQYNIIQKKQSKTFVFNSRHIIEYQQQSPGPGAYDLNIKSCRSISIGTARYQSQTDSKFQTPGPGAYKINQKVNKKKIDRIERLLKPKIMVRSYYNRKEDKYIKELMKQKETQEQQENKIRPKSAVQIKQTARRKKMEKIRRSFHEKCNFPGPGSYEIKDFIQIDQADKKNQHIQKSNSEKKLKSDNIIGPGSYEIKDFLYQSEYIKKSKINTSFGQSKRFQVEKISVLGPGSYELVDEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y0X7</accession>
    <name>A0A8S1Y0X7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1480049</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000148">
        <property type="protein sequence ID" value="CAD8207661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y0X7"/>
    <dbReference type="OrthoDB" id="291692at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="584"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8207661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="708" mass="83745" checksum="33D350BD6D706745" modified="2022-10-12" version="1">MGQQIIGKCKQCGPQNEDILPQMEFQKEIKSEVIKNLKQKLHEFESYSIQKLTTYSLKYDGLFRKEDIKKEKVIDSLGLVKPIDIEKIKQLNEEVGIKFYCGISLGDPINIGFKTINGSQIELNQYGYTLVRLWRMGEMMMNFQSCIQIEGIKLIGLVRGDIETYKKIIMNIGWEEYDHIILEDDNILDLQYDEQTHQINHNSMLSALLLYDGQVIYKHYIDKLQFQSDYNIVKKDVLEIIENGVKQANQINVITNISFIKQQEYIKLKQFIMNQKKQSFSRDVEVTIKKKTIYYKEDKQVYYDQPIIYINSSPKSFSISDKFANQLGKKNLKWDSKLLKAKINEKIKEFSLNVQIALQKDIIISITKEYCEIKVHHWEDGILVPYYKSLDYISQIKLWITATETIRYEPNEDPVLDRISDLVAKLFQTIPKVDKVLGTKYANIPFVEKWKPPYEELDPHPKNLEIHRKSDKILLIWLFDYEIDENIEKLKELIKIKKNNPQFGLQIAILGYIKFTTWTSKFQAFLDKCELTRSILDNTIETWFPQEEQMGRFRFNIILQRIYGLMLDKQDMMIIDSKGIVRLIDNSTQLISNLTQEIDKLNEKVKVTVKNYNYWRQYQNLKKCIRENPQLAEIVKKIQEKGIVEESKVLIEQMKDKVWTFENGQIKEEKWHEQPTRILRNIQDFTDINEMARIIAKYIDNNDICALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y158</accession>
    <name>A0A8S1Y158_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1460024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000146">
        <property type="protein sequence ID" value="CAD8207045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y158"/>
    <dbReference type="OrthoDB" id="18915at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071108">
        <property type="term" value="P:protein K48-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22749">
        <property type="entry name" value="Otubain_C65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1300.20:FF:000006">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019400">
        <property type="entry name" value="Peptidase_C65_otubain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12931:SF15">
        <property type="entry name" value="UBIQUITIN THIOESTERASE OTUBAIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12931">
        <property type="entry name" value="UBIQUITIN THIOLESTERASE PROTEIN OTUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10275">
        <property type="entry name" value="Peptidase_C65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8207045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="454" mass="54690" checksum="8DEF3057B49C3516" modified="2022-10-12" version="1">MNEDQTKIEIEEDSNSLVPLLLILLVLIILFIQRWSKKPQISKQYLVENDEHELQENLLNKDQQKKQDNQCQKNYYSVFNEKQNNQNEIFLKFKELQEQIKVQYKPQIYNQIQKPQQLICNNSINKIKEIYQNSSQLRFVEDEFKYYIRRFQEKSDLKRNYGLTFQASSKLYEYCNAFREVRGDGNCFYTAFGYQFLCILLFEYTFDQFQEFIDKIRKSDLPMKIFVTDKDLKIDDKQLEKQLLEEFLNRLTKLKQIDDITQRQDSFDTQFAAYEKQSEEIDGCLYGLSTIFFRNYSNYIVDLSINKDAVYDRENLLKWEEECNSNEVVIAELAKQLNVFVQLIFFEDKNNFVVREYEKDKQHKIILLIRPGHYNIGYYQQKNIIDQCLELVQKLNGIFKNSSEQVDDYQKDIVQELTKKITDLFDEIQILIKNQTLKECDAFKLLRFQLQSQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y350</accession>
    <name>A0A8S1Y350_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1480047</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000148">
        <property type="protein sequence ID" value="CAD8207657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y350"/>
    <dbReference type="OrthoDB" id="307388at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8207657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="386" mass="46663" checksum="D02E1BA0D9A700A8" modified="2022-10-12" version="1">MIKIIFPQLNNHTYEIKNPDYQKPMKFYFDEAIIHLQKQIPQETKFCLTINDQEKDMTQDLIHLQINTSSNIKIILNIQIQITITFQTMEEKVTLVFNSFDNLYKLEKQIKKTHNLVSYDLNFFYKQQQLRKDISLKALSIQNDSVINCQIMGTYSINYQVEQFIIINIFSTIDQVYQQIQNYFHLKQEFQLYFQDELLKQRQYNRDILFYEYKIKPYSEIRLKLSDNFTIEVFIDSIKMLQHYTVSKNTKIGDLKNYLIEQCQIDKHCSYQLFIEDQILSDDYQISELTKIYKINSKQLNKIFTLKVVDKSITFYICKKELDDFKIRVIMNQNNQIQDLQNLKQLEGYKCQFYFNNQILNNHKTFKQINLNNQDIIYYEIQPVLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y7M2</accession>
    <name>A0A8S1Y7M2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1550062</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000155">
        <property type="protein sequence ID" value="CAD8209600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y7M2"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8209600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="169" mass="20193" checksum="942E0832943337DD" modified="2022-10-12" version="1">MQLLKEIVVNVNNPINSIEQLKKQILLKFNKQQYNTLDQTHHRSISTQETPFTVISIKKPKLQLNTIELIPLQNKTRFMPNSTLELIVKQQKNKSFQFKQKIYTQESKEKSEYVKGRKRFNKNVECLKQNNQRFRLSVSVNEQSQQLNKLLESRLSNIMRQNNHDNYLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y7X1</accession>
    <name>A0A8S1Y7X1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1600001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000160">
        <property type="protein sequence ID" value="CAD8210566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y7X1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8210566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="143" mass="17176" checksum="398091B318540061" modified="2022-10-12" version="1">MFYHNSRAQLYTVLFSIWEIRVLVSKMCILQRIMGQVTPIAIILANQLCQYQHKNINLKHEKFCFGNMTILKTIYSGITILVFMLFQMKKKIKFTKVNKQVAQQQLNFQINLFKNFSYQMQEVIEIIVKEELYQQLWFTHNQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y9Z7</accession>
    <name>A0A8S1Y9Z7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="18">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PPENT_87.1.T1610046</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 18">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>Na(+)(in) + ATP + H2O = Na(+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:14634"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Membrane</location>
            <topology evidence="18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="18"/>
    <dbReference type="EMBL" id="CAJJDO010000161">
        <property type="protein sequence ID" value="CAD8210885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y9Z7"/>
    <dbReference type="OrthoDB" id="3352408at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008554">
        <property type="term" value="F:P-type sodium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02081">
        <property type="entry name" value="P-type_ATPase_Ca_PMCA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000039">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000061">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000045">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000191">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006408">
        <property type="entry name" value="P-type_ATPase_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01517">
        <property type="entry name" value="ATPase-IIB_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF369">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 18" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 18" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0915">Sodium</keyword>
    <keyword evidence="15" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="302"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="345"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="798"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="832"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="875"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="914"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="954"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="986"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="20">
        <location>
            <begin position="41"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1043"/>
            <end position="1067"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAD8210885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1067" mass="118605" checksum="D89760AF329EE3D2" modified="2022-10-12" version="1">MSYKQQKDVEAQFQSQLTIDDLTDLFKLNSINEGISMQKVKQYGDDVGIARKLNTDLKKGFSDKGAIEKSKQVYGDNTPVEKEPTTLCELIMECLGDTMLQILLIAALVSTVIGMINEGVKTGWTEGATIFFAIFLIISITAGNNYLKERQFRQLRRKLDDGKCQVIREGKVTEIQTKDIVVGDLLIFNLGDLFVVDGMMVQGSAVKIDESPMTGESDEIKKLPYNEMAQQQHNQLNVNQEAARGHVSPFLISGTKCLDGTGQMIVLAVGQNTVSGKLKQLLIQENPPTPLQQKLEGVASDIGKLGVVVSILTFIALMGHLGYDCYLGKFPFLSIKTLQILVESFMISVTIIVVAVPEGLPLAVTIALAYSVGKMKDEQNLVKNLSSCEIMGGANNICSDKTGTLTQNIMQVVALWTEQEIFKDQVHTNKNKIKKETIEFMCESICYNSNAFPEKDPQTNKWIQIGNKTECALLECSDNFGYNFNQFRPSDKVLRQIPFNSKRKKMSTVIYNQKSQYIRVYTKGASEIVLSQCSKYIGKNGIEQLLEPQLKKNIYDNIIQKFASDSLRTIAIAYRDLDPQSHGSNVRGQISQLTKIAQNIPEDDLDKDLVLIAIAGIKDPIRPDVPNSIKQCHASGVKVRMVTGDNILTATAIAKECGILPTNREISEWEVVEGKKFREFVGGLKDEIVDGKTVKVVGNKENFARVSRDMKVMARASPEDKYILVTGLIAEGNVIAVTGDGTNDAPALKKADVGFAMGITGSDVAKDAADIILLDDNFSSIITAMKWGRNIYDCIRKFIQFQLTVNLVALFMSFLGAVVLKESPLNTIEMLWVNLIMDTFASLALATEPPSITVLERQPYKRDDKIVSPTMNRTIVGGSIYQICVLCGILFLLPQYMDLSMPAELIGQKFHKNVVQMSIFFQTFVVMQVFNSISCRQLDYKTINPFANACNNPLFWGVQTFTLIIQCVLIQYGGKFVKVSHLTLQQHLLCLGFGVGSIIFSILVKIAVPERWCQFVELFRESELQSGDMDTSLTSVLRRKSTNRLGNSKRSIDQQGSQGRMSAQRMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YAR3</accession>
    <name>A0A8S1YAR3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1510060</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000151">
        <property type="protein sequence ID" value="CAD8208554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YAR3"/>
    <dbReference type="OrthoDB" id="21530at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="74"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8208554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="111" mass="13031" checksum="10E4F1A2C9103723" modified="2022-10-12" version="1">MSESESEKQEAQMDYYKEALEKVMTERNDLKTKVLKYELLGGELAQLDDDEIMNQLEDRKKKSRRSAADIDRHFFCSFTNCKKAYGTEASLIQHQRLKHGQNNGMDAYFRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YAX5</accession>
    <name>A0A8S1YAX5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1510090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000151">
        <property type="protein sequence ID" value="CAD8208614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YAX5"/>
    <dbReference type="OrthoDB" id="10350504at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8208614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="259" mass="31425" checksum="6C40EA51847349E5" modified="2022-10-12" version="1">MFCEHKGNLPELLNMALSSKYQTDFCQFQLHSPITIQFQDQVCFEEQEEYFKFYYTYPQLKDNQKHLTEYYRYHRDIPRIFIKNITSQLEVYHEKQREIRYKKIKKQLGMQVKDTTRDDIIQPFKALKGLSELQTSRQLQQLLKQIKPLHYDKSGIATLIQQQKQQMLQSPNLKRLLKMDNIKTTTTLMTTKRTHHTYHTIQDIKKKSHKKQLTLCTEYSPDERNDFYKRWSDVLSHRQLQYHKNKIKCTLLNKKKSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YBA4</accession>
    <name>A0A8S1YBA4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1650024</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="EMBL" id="CAJJDO010000165">
        <property type="protein sequence ID" value="CAD8211756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YBA4"/>
    <dbReference type="OrthoDB" id="5977743at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="267"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="8">
        <location>
            <begin position="158"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8211756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="382" mass="44810" checksum="8EB22D3F349FE52E" modified="2022-10-12" version="1">MEFLAILTLIFLIIGASLYLLVCVDPNSPGLLGIMNRFVFNTIPTIIKKILGERIFTIFQRAVDYFFYSNHPLVQIFYVLVAVGGYLVYLYFGFLELFGNNPFVSHLDTSIGSTMALFCFYSFFQACRYKPGIITKENNKEYVNEFKEYYDNVVYLKENQCKTCNIIKPARSKHCRVCNVCVSRFDHHCVWIRQCVGQKNYKYFVKFIITHAILCDYGAYLGFRCIWGIIVKEKLLEAQFRDPVTKQRLQATWGIIVKYLFYKNTMYIFIVILCIVMGIALTCFALYHLYMIGQNTTTNERMKRSDFLNFFDEETERLEKQLKDAQTPEDIKQVTSKLEQVKQCQNRIIPAKSIGIWQGLKRVFNEPDELDQTIKIKNRKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YCX1</accession>
    <name>A0A8S1YCX1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1640069</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000164">
        <property type="protein sequence ID" value="CAD8211609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YCX1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8211609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="192" mass="23232" checksum="F2F8B744CEEF327D" modified="2022-10-12" version="1">MPISQQEQSNLIYMVQIYKDGRLRELEVGVFLNKLLQWLINFLYSVTYGKQEVFKNPMQEDGLINFTQEDIQYLSLVHLFLKQLMPLYICLRPLLIVRRYKLQTLELKNKKEHKNSQNYIKQTDEEDQLQKILNCYYYYIIMLRSIYQDSLQLYLIIFNFKLQQPLNRYHIFFTLIISITFSAQIFKSSDAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YEB5</accession>
    <name>A0A8S1YEB5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1640029</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000164">
        <property type="protein sequence ID" value="CAD8211538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YEB5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8211538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="212" mass="25728" checksum="2F36D1A30BB2217E" modified="2022-10-12" version="1">MKEYFQQFGLIKQLILRNEQDEKVKDETKVCFTLAFTSFDAQLILQEYENIQVGYQQSKFNISDSQIKIQKLHYKHNEYEKILSELYEEVFCKKEIQTIEIKSNILKNKEHFQSFDQVIKELDHFQQLSQLDQKYIEQQLIYKIVANQSHLNLVDQKEYHVQQQKSIRNLKILLNFTKIQLNLSWEQKKLKIILFQIYIQKSFSDIQHQMKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YQ44</accession>
    <name>A0A8S1YQ44_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1940016</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000194">
        <property type="protein sequence ID" value="CAD8214042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YQ44"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8214042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="62" mass="7382" checksum="9738FA4FD40520A2" modified="2022-10-12" version="1">MIHQIQTNKDQVLNNCKTEKLQKQMLEELSHSIKQIYEMIEKENQSFIDLINYNENLAESSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YSD1</accession>
    <name>A0A8S1YSD1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T2980002</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000298">
        <property type="protein sequence ID" value="CAD8215044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YSD1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8215044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="59" mass="6952" checksum="ADFF5026EA07879C" modified="2022-10-12" version="1">MLGCLSDISYSDKYKNTEFEYRNTISPPKIYNKSIKLDYFLLNMNGEVLAQRISRIDQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RUK0</accession>
    <name>A0A8S1RUK0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0010251</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RUK0"/>
    <dbReference type="OrthoDB" id="284828at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8131718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="526" mass="61402" checksum="B168D2FD12AF63DA" modified="2022-10-12" version="1">MFHSETLQEHILYDKIYQYLWVKSETSNPPSILIPETVILIRSMPIYWYFTDKASGEVKRKMHKNVSKENIKESWLNSVGKSGVVGYLLHFIENLDLNYPNEKLKVCGQIQIIYFDKEGFIKFMDSNVQFPFGILQKYVESADDKNSQIQALWSKQITLFTKRTTKKSYLNKSMNIYERLCTFEGPDYLSEATQVKDFQSQRISEQIQKMINHLDSISFGKLNINQGIFYFKVDREAKCWFLFCGNLKFEGEKHLKDCPKDLYSQAQIKIPKSIDGIMSVYSQKPQQLNKESKCIKCGIIEKENNFIMIPYHYILENDQNTIIPQNWPNEIKNGATKIKLVNKNSDSSDLLNTISKVPLVFQKIHEKLNYQSFDQYKNNDGFLHKTLQVCLNCYIILVALQEKKQKDCVINSISPQMKIKKSLGGFTSAKSLQQYLRPDIKEKIYTQVSDTCRKYKNIKIRPVQQYVQKRLQSLSPTQKSSMDFPSSPDLLSYHTKSSTYELGNNNRKLRNCYNQSLQVKLNNLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RXG5</accession>
    <name>A0A8S1RXG5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0020592</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RXG5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="291"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="334"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="423"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8133751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="518" mass="59899" checksum="C0DE2ACDAD192A96" modified="2022-10-12" version="1">MRYNIIFPIVVRQNIQGVGDIAHIAKFAFQASIQLIPFTINVQNHVQIVIRKQTFVVLELILEIKHLLNVIVLMDSQWILQITFAFNVNFHVLHAKQQWIIAQHAMMDILMSIISANNANIHAQNANYKMIIVLNVQIQIMNQSVINVYVNMDLALQELVISIVHSVNIHVLNAQVVLTLVSLALIIIDSNLKIANVYVSKVILKQELIVYSVHHNVLLYQNNLINVYHVLTLIKYYNQMIVIVNQVIIQIIIRIVYNVNILVQLVIKIFAYLVKMDIILLKDNVNVKKDIMILIFIVKNVITIVVNVSHHTNVPNVQINIIFKILNVQNVKSHVYKVLIFKLVKLVLIIISLMVKENVQNESKIVNIVLIQLIVLSVLMNSIIIVKHVSLVLINVRHVQKIVNFVHLVKIQIKYQIKRQVCAIKLIILEISMIIIIWNKYYYSKISDAIKINYQQVMNVLINVEMVYLMNNMNNVMMVINLEEMDAPSFVFKRIPISAKTMQILHVFVHLFKLQILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RYC4</accession>
    <name>A0A8S1RYC4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0010587</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RYC4"/>
    <dbReference type="OrthoDB" id="296186at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8132390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="269" mass="31895" checksum="DE35E5F29C9D6805" modified="2022-10-12" version="1">MDSSDSQEDSYQKDSLLEILQFGQFKKQNESEKLSIDISNHVLNLGMFTKPKQNYIQNTNHTKELYISIIKKITDQLVYQDQRNLFSPKKIILGLQDYLLEKQVERIYNTVRRKSCICTKCGHQSDFEKRTNSFSVRTQQQINTQIRLLNYNTRNQFKQQSLIRLHKIKQLQNQCHYSSLSAQIDNQLQFNQTPLVEPTIVNIKWKFKKKTIIQPVVLKQYLPTTKNYFVKQYHLTSRTGTNNTPTQKTLPSLKTLTQSRRNLFDNKYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RYD0</accession>
    <name>A0A8S1RYD0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0020196</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RYD0"/>
    <dbReference type="OrthoDB" id="308132at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001447">
        <property type="entry name" value="Ras-related protein Rab-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8133038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="219" mass="25636" checksum="95826E4FE92AAA4D" modified="2022-10-12" version="1">MKNQEFQQQSIEDQITLFILGDNQVGKTSLIIRYTQDIFDEVYNHKKTLNCNSLLKNDVSKSIFIENQCVHLQIHDISGPEKYIFIDRVYRKAQGIFIVYDVTNRYSFNSIQQWISQIDNIANEGVIRILIGNKCDQKDNRAVTFEEGNLLAQQYGIPFFETSVKLSINIENSLIFLIKLIMQKQEQQQQQQLIIKNEKKNQKLKLKQNNQSKNIQSCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S0U0</accession>
    <name>A0A8S1S0U0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0030550</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S0U0"/>
    <dbReference type="OrthoDB" id="301970at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8134881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="110" mass="12566" checksum="51CB71FF46266488" modified="2022-10-12" version="1">MIAGYLQNKLIAQSSIQEVHGTDMLATQDRRTSKIYMGGQSHQQMNPLDLVYISSALPQLYLQQNVDPKLQNLLNTNYQQQISQLASPPNIELINQIKEQARVVRMHYQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S204</accession>
    <name>A0A8S1S204_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">DNA polymerase kappa</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PPENT_87.1.T0020493</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Endomembrane system</location>
            <topology evidence="15">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S204"/>
    <dbReference type="OrthoDB" id="447129at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005657">
        <property type="term" value="C:replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070987">
        <property type="term" value="P:error-free translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009314">
        <property type="term" value="P:response to radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01868">
        <property type="entry name" value="Rab11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000211">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.60:FF:000008">
        <property type="entry name" value="DNA polymerase eta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000067">
        <property type="entry name" value="ras-related protein RABA1f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052230">
        <property type="entry name" value="DNA_polymerase_eta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041298">
        <property type="entry name" value="UBZ3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001126">
        <property type="entry name" value="UmuC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45873">
        <property type="entry name" value="DNA POLYMERASE ETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45873:SF1">
        <property type="entry name" value="DNA POLYMERASE ETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00817">
        <property type="entry name" value="IMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18439">
        <property type="entry name" value="zf_UBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50173">
        <property type="entry name" value="UMUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51907">
        <property type="entry name" value="ZF_UBZ3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-0636">Prenylation</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UmuC" evidence="17">
        <location>
            <begin position="214"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="UBZ3-type" evidence="18">
        <location>
            <begin position="650"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="680"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="694"/>
            <end position="718"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAD8133612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="736" mass="85458" checksum="66A63F45AA31ABE0" modified="2022-10-12" version="1">MSKAEDDYDYLFKIVLSGDSGVGKSNLLLRFTKNQFNPDSKTTIGVEFATRSITISGKIIKAQIWDTAGQERYRAITSAYYRGAIGAVLVYDITNKQSFEAVERWIQEVRENADKDIVIMIIGNKSDLKHLRAIRTESGQDLAQMQKVAFMEASAQDGTNVDQAFTQIIQYVTFIRREIRFRNYKLRQQKVRMLLKYNIFFFSFKFMNSLLQVYIHIDMDAYYAQAEQKLLNIPEDQPVCARQWNSLIAINYPARDAGIKRGMLSEEAMKLCPNVMLPHVETFKVIDGKMVFSTLKDKYIQHNQYEEKVSLRYYRQESKLIFATIKRFCNCVEKGGTDEGYIQISEKELENIETQQFYGHLMKELPDEYQLTEQDYLLMRASLLCQNIRDAIYQETKYKCSAGISFNKMLAKLASATNKPNKQTIILECMLPECISNISIKKIRGFGGKVQETLLESGLKTVGQAQSLSIYELQSLFGDKAQYIYDKLRGYDDELVKKEVDLKNKSILSLKNIKKTSSREVIIQSLELIIHDITMRVTDYYEDSNLVPSVLVIHYHNVEKGSHQKSEPIYLTLPIESFRLTIEERVLSILNQIQDNELFPLIHIGISCRYFKPMIQGIQNPITMFFKKTLEQQEYEKQAKLAEMQEKMISPDDYYSCQICGQEIMKKDKEAHDDFHIAENLDKEMNPKKRKYKSQQQQQQQQQQQQNNSNQQSNVNSKSKSKQKGLTSILQFLKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S337</accession>
    <name>A0A8S1S337_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MFS transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0030396</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S337"/>
    <dbReference type="OrthoDB" id="6415790at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008521">
        <property type="term" value="F:acetyl-CoA transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035348">
        <property type="term" value="P:acetyl-CoA transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024371">
        <property type="entry name" value="AcetylCoA_trans_1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004752">
        <property type="entry name" value="AmpG_permease/AT-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778:SF9">
        <property type="entry name" value="ACETYL-COENZYME A TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 33 ACETYL-COA TRANSPORTER -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13000">
        <property type="entry name" value="Acatn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="174"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="325"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="357"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="387"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8134616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="442" mass="50803" checksum="69009D6627859FDB" modified="2022-10-12" version="1">MVFIKRLVPLSIFYCFEGIMLGLILYSVPSNLIDKGVEINRYNFSFVVLLPYLIRYIIAPLIDSVSWDKIGRRRSQLTVLYFGLGVLLYFSNFQSLDPSTWFWNTFWIVCFLACIDLIIAAWLVESLDKQDRGYGALAQFLGILIGGFIGAYIFKMFNSLEFCNTYIYAIPQEIPYITLQIAFKDLSYISVGLAVACTIIIHNEKTTNQDIIEAYKSALGAFSNRHLIVLFLFFCFFRIGQMPIDLAHIHLKRGQFTEGQIQFLEILTFPIACILPYYMAKMIKTRVLEIRLIIVFSIYEIAISGVFIITLFASEENFPFPYRDTLLKISLISVWLLNLINITLAQSFIYKSTQSSIAATFIALLTTGINLQFIFEGLVEYLLANYNYYLIWICGVGIYALFFFHLASRATMIDQLNCQEFCLEIDIKKEKLLINTELATLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SCE6</accession>
    <name>A0A8S1SCE6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0060182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SCE6"/>
    <dbReference type="OrthoDB" id="295074at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="358"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="363"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8137618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="383" mass="44070" checksum="9A75CF6EA5419596" modified="2022-10-12" version="1">MTDLIKQLDGMFTREHLEKNKYLLKNLDPNLNLPIVALQKEYSLAKHSIEEILGALKQCKNCKLHEGYVTLLIPPHTKNIIINEISQTNADFKAYLIEKYLKNIECEIAFEGGCCRVTFKDDEVGLKFLDQAVNDPKDPLKAYVEPENVYQSLTKTLGFNNQAQYLYQYNNQMFQNLYIRKASDDVAGQNQSEKINKPYYPPVQQQQSNPRKQSGQERKGSEQVEHQDKKTKTPTTGPKEIYVEKQSLEKQEQNVKNIEKPPKVQQSPYVEKKSTSRKTSEQIQPAKHKGSKDIQGSPIRKASIQYKKDNLIEIFKQIQGTLKANPKLQKLSEQDRALLLRQNGELTLEAIHPTPCIRKESYASPQQHIKQSPAQIPQRKPQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SDM1</accession>
    <name>A0A8S1SDM1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0060429</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8138058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SDM1"/>
    <dbReference type="OrthoDB" id="305151at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="85"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8138058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="205" mass="24292" checksum="5174B21A479223AE" modified="2022-10-12" version="1">MSKKVKQLAEVIALQEKTIGKLQKIIENKKSKQIGSRKNQSIKLDQKYLVGTNAKPPRLDFNETNRSYRCRPSISQTHNQLSQISTDYIQDIQSLRGKIEGLQKEKQDRSLFFQDKENWNNNNNYQKIETLEKQIKDRESTINVLQEQLVQEKQLRQQTQQDLKQQRTIWNKMYSELSNEIKTLKTELRAFSSQPKKRSNQLFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SKC6</accession>
    <name>A0A8S1SKC6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WD domain, G-beta repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0080300</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SKC6"/>
    <dbReference type="OrthoDB" id="295882at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="479"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="520"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="562"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="639"/>
            <end position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8139799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="765" mass="89601" checksum="59A57AD1EB80783E" modified="2022-10-12" version="1">MQYYCQLHQTIFTQVCIQQDCTKKGPLCNKCIDTHKNHVLQNLETYLNDYTKYLNMEYQAQILQKLEQMKQYFHDKISSLQIDVNYIDEKRKKEIIKQLKQEQMSPQQYESKMIELIQRDQLKDREYLQQFFQQIIEIEKEMKFQFSQLPKKLIQAQYHHQPSHQSQQIINNKSNLSSEVRELSPLLSNRSNKSKEPIFSQKQINQSPLSKQLLQRDLMPVGSFNNSKLKNYFTEQDSQISPQNSFKIIKPFDIDKSLSNQSRIEGQKSKSQNTSFDLSKKVEPFKNTQQSQIIEVPLINKYEKSDSQDLYNQKQRLINPQELPQQQIIGYYTNQKTLTQKENNETHQKSPSNNKAQFSIKEEKLIQSNSNQQQSEIYYQFQNSQANESSNFDFRQRFQNQDIQQFQTPQQSTLFREQETNNLQNGMKLSTQSAISGIYNENKSQQDISFISSKSLLSPQIKITQNLPNPLKIEKEDTLKVHDKSVKDLCFMDDDKIITCSKDQSIKIWDKRTKQLKMELKDHTDQILCIAFSLKRIIVSGSVDKTIRIWKPQPSWKIAFVCQGHSERIRCVEFVGNLIASGSDDTTLKLWDFDGQLQHSFKTNGRVSAMVTDKNLIIVGCGKNILVLDTRTKNKQQDIIGHKNLILCLQLSNQNQQNNSILISGSKDNSIKIWSYPKIQEIRALQDDYSIHSLFFDPNSQYLFVGLMGFEQEGKISIWQLDNQPKKIQEIISNQYGCNKVLYDGLYLYSAHENKRMEIYSIKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SLK8</accession>
    <name>A0A8S1SLK8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0100054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SLK8"/>
    <dbReference type="OrthoDB" id="296513at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33209:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_55, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33209">
        <property type="entry name" value="PROTEASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="467"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8141323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="506" mass="59904" checksum="39B430C9F0698C89" modified="2022-10-12" version="1">MFLQGSKIKTLEPTVNQQMKYSTRQLNQEPKSSHSLHRRYSTIGVIHQDVYKIEDKYSKKLNHLLQTKYYNMHQLFKKKQYIPANFKLDNKPFEHVVTNQFSKTSSQFVQKKKKFNVKEWHKINYDRFRFLSSLVYKFHRLIDNIDQSRKQIIIELNHLIVLIMTGVRVKDVILYKKKILLYFQLTKINTTKNMTELLRLYENNFTIILYLFIIIMSQLISKIFFQKTVLVLRIQGSINSQLVSQISHQLEHYNRGSVVALGLIINSQGGCPYQVEVLKKRIKNYLNDLPLYTFATEHSLGNAYQLLQLGNKSYAQNTSKIGFTHMYQNKLNFADFLKNYGINTLQYQSSQIVNHNFVVNSSIKSNNQQEINYLHQYYGLKSKQLTEECWTSRREKIKINKEQLENNVFLGVEAKELGLVDELGSYEKVLMQQYPKAKIIPINTEGKLREYENWFNLFIKISQSRGFLGLVLLFGVKVMVKWGLIIYVWRQSVKQRKLQKQQELQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SNJ5</accession>
    <name>A0A8S1SNJ5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0110068</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SNJ5"/>
    <dbReference type="OrthoDB" id="10301282at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8142295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="170" mass="20853" checksum="B430F3663FADF5B8" modified="2022-10-12" version="1">MIDYKLLTEFEEQKTQFLQRFIQNKQNKQQSKDSKTELIMRKLEKERLQTPFVCYQFRVKKLKTFVKRTSYNSYSFINNQKVQSKDQTQYDNKKRNNSSYDFTQHINQVELNVQNKEVDTLFRPMQDRRQKTPLKMKLQQLEYGKNYQKLVDKIIELDKNPINFKKNLYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SPT5</accession>
    <name>A0A8S1SPT5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0100323</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SPT5"/>
    <dbReference type="OrthoDB" id="285200at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8141818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="238" mass="27940" checksum="77382DB29EAD7F10" modified="2022-10-12" version="1">MQNILSQQQQFFDLLDKQVDAYKTLLDQLSQSQDKFNNLLQDYLQKFQTINNKLVQGRQDFSCSQIQIKRNDELYTHSQLHIQFNLRSLLEKQYKYKLTLADSLETPLYRDRIFQLRVELKNKSGDLVKNPNKIELVVAIYSQEQYPKEIKVNNKGEQILKGHLCVPLIKGTAFFTRVQIREVSSHYQNGSIILAILPQFKSNDEPINSRDVQPLIVENIKVKAKKFSERHIPVYVNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SS80</accession>
    <name>A0A8S1SS80_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0120040</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SS80"/>
    <dbReference type="OrthoDB" id="93876at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042234">
        <property type="entry name" value="WDFY1/WDFY2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46189">
        <property type="entry name" value="LD41958P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46189:SF1">
        <property type="entry name" value="LD41958P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="PX" evidence="3">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="419"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="483"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8143103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="503" mass="58429" checksum="32840377AB2817D3" modified="2022-10-12" version="1">MNESINVTIPLYQQESSNVQYVIDIKTRNKQQWQISKRYSQFEDLHKKLIPLFQELPELPKKAFITFLVGKSKEQLEERRAGLEKYLQQLIVRREIYHSQLLRDFLQLEQCEEILPPNLLHSFKTIMGIRDISLSDQGVMFLLQADMSVLNRVDAYVNNMKMPWDSEQGEVKTIPVGQVECYIKQEEGEFCYKKVWTKEYSTQAICMFYEPITCTLLIGLDSGNINFIKVSERDSFEKYEQSIEIEIHMSRIMGLFYRNGQIHSVSKDTHYKVIDMEQGGLKSDFSIGKHELTSLTYNEQRQISVVGNRNGQIYILNIKPAQPIVMLQVETSTSFIRGLVLDYQKNYLISVSYDDGVICVLDVGKCLQDNVGKIKTKIQAKIKTREVQWSSKRGELFVGNDDGTVTIYNALDLQPQFVLKAHDKAITKLIWQESTQILITGGKDEIIKWWHFPKKWKAGDQDEQVKSKQLIEAKMENILKLQNQNQKIDSDEEDETGLGNWHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SV80</accession>
    <name>A0A8S1SV80_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0120079</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SV80"/>
    <dbReference type="OrthoDB" id="291555at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8143179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="391" mass="45951" checksum="287B287C2765CD5D" modified="2022-10-12" version="1">MNKLNPMDILPIIWPQKISARQQEIQRVKQELMQLKKDQPNLAQSINYTIQYLASHAPYIDVVVSFQNLVDIKHPIYDLLNYLSTFSENTLTQVVRKVNLNDLNSDSDETDFTYSDPYEEKKQQFNLKMMKRVSFPKLQKLISDIYRLEMLTKIYSWINQITYVDYVKVSVSTIKKALQTEYIDELTINTVIQCAKGSYQCVNQYLQDTPKIKKLKIALHKIAQLQDVDLQVLLTLQESDQKPIKECVWTRQQVIQCILLFCLVQCELLIHDSQFQIIQSKPFCTFEISWNESGFSQIRDYFPKLAKKLNKRYKILKDIPFYDEKTNSSIFIYAQSLVGYQHHNYQNFQLSDNLKQTIKDSLQNQNQEKNLALQIIVSNAIMFAKLLKALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SXB0</accession>
    <name>A0A8S1SXB0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ribosomal RNA small subunit methyltransferase NEP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0120495</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase NEP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SXB0"/>
    <dbReference type="OrthoDB" id="269804at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070037">
        <property type="term" value="F:rRNA (pseudouridine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18088">
        <property type="entry name" value="Nep1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1280.10:FF:000003">
        <property type="entry name" value="Ribosomal RNA small subunit methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005304">
        <property type="entry name" value="Rbsml_bgen_MeTrfase_EMG1/NEP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12636">
        <property type="entry name" value="NEP1/MRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12636:SF5">
        <property type="entry name" value="RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE NEP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03587">
        <property type="entry name" value="EMG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="8" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8143969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="225" mass="25540" checksum="C42B26D4969EE56A" modified="2022-10-12" version="1">MQQELLLPEDIEDKRQLIIVLEQAPLEIATIRQQIVLLNADEHKNYINQTLNKDYSLYRPDILHHCLLSLMDSPLNKAGKLKIYVRTAQNVILDISPKLKVPRTYESYAALFAQALHKLRVRAVESSETLIKVIKNPITDHLPSEALKIGTSTQAKLIDVKQFIKQPNLNQNKPIVYVIGAVSKGNPGMEAQYIDECICISQYSLSAGYCLQRISNAYEELWGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SYD5</accession>
    <name>A0A8S1SYD5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0130162</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SYD5"/>
    <dbReference type="OrthoDB" id="285802at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20528">
        <property type="entry name" value="CYCLIN_CCNJ-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin N-terminal" evidence="1">
        <location>
            <begin position="38"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8144306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="334" mass="38543" checksum="9465A3DD160B8FBC" modified="2022-10-12" version="1">MINCQHKPNKNICKLCGIAIYKDGSVAYKHNSFKSHQQAKNIYEDLSNINTNYERREELIDFLYQTKQNLQLTDQTVHLAIHYLDRCQKLLQNHNPKLISLLCLMVSSKMIEDDLHIPGMSKLSKVSDQNLNKNEYKQAECLVLKTLNWKLKVNTLYEQVQSLLCIGVVFSTDSNDGIMDSNIINFVCCKMLKDHSKIVYQVNKIHQLMQINTKILAMAIILYMRKQCRLHPLPHQLQGLLEENQNLLLIDTYGQLNNLLQNNSLSNTKSVSRALSPITLSTCKSMPPVTRVTQNLRMPLNIITNNHNCIKPKLIKSKHQEQDLNPLRVKHKNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SZ60</accession>
    <name>A0A8S1SZ60_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0140274</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SZ60"/>
    <dbReference type="OrthoDB" id="289047at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398:SF0">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8145500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="480" mass="55917" checksum="F7137E1418E73F90" modified="2022-10-12" version="1">MYIIQSAAQNLRTLDIFGMDVHLLSKGSTTHKTYSGAAASLFIFSVLIYSFYSFILQMNEGKNAILNRQEALLQNNEGYKFDSKDFIFIAGLLDAAGQPIMNQNNSIYSVTYYFCNKSLSDTQCTYFSSTICGDIITELSQKMGVPKDYQYISYCIDQEKTDNFETLRLQGSQRMDNFTLVGALLNKCVNNTEKNDCAPQELIDQIVTNSNFYYSYTHYQFRKDLDSYPFETMQNFDVTTLYPKLKKYIKVLYSYSVAYLEYNPFYFFPQTTECQSVEYDQSIIDAIVSSDNDIQLAQVEIHLGVKKYISHVTYQTLMDVAAKLGGFFTILKICVSIMLYPIQQWQYRLYLINCYLDIKKGSSAQSLTNFMDHDKLNLLQVALQKKKRDYYHQQNRIIEKLLDVVEIAFNPLRLSRQVEDLQYSIKQFDISKTKRFRIDENEHEHELIEIKSISRSINQEFEHIGQQNRIPRIQVNMGNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T033</accession>
    <name>A0A8S1T033_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0130435</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T033"/>
    <dbReference type="OrthoDB" id="10266771at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021910">
        <property type="entry name" value="NGX6/PGAP6/MYMK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36561:SF2">
        <property type="entry name" value="HAEMOLYSIN-III RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36561">
        <property type="entry name" value="HAEMOLYSIN-III RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12036">
        <property type="entry name" value="DUF3522"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="189"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8144856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="233" mass="27942" checksum="F2CBF14B6BFC37FC" modified="2022-10-12" version="1">MTDIQNIVHSKYGYNQFEMFLMVLITGITNFVMIPVIIIIRKQRMDCQYYISIFTFITSFMYHTLESVDCEYFIIEEGGWHRLDNIGSIVCFQMLFTQLSDFQNLHLETMLNYFGLFITFIAQAKSPWDLNYTVGPILIQFLICIFLIIKRRRLPKLNKKKMKKGLIILLAAVVFFCISQDEYKDYLRFYHGMWHTTVGLFSFYVWQSKCEIEFTLRNFYTIPTLKESYYKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T135</accession>
    <name>A0A8S1T135_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein SHQ1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0140153</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SHQ1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T135"/>
    <dbReference type="OrthoDB" id="73639at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000493">
        <property type="term" value="P:box H/ACA snoRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039742">
        <property type="entry name" value="Shq1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048696">
        <property type="entry name" value="SHQ1-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007009">
        <property type="entry name" value="Shq1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12967">
        <property type="entry name" value="PROTEIN SHQ1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12967:SF0">
        <property type="entry name" value="PROTEIN SHQ1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04925">
        <property type="entry name" value="SHQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21413">
        <property type="entry name" value="SHQ1-like_CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SHQ1-like CS" evidence="4">
        <location>
            <begin position="6"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Shq1 C-terminal" evidence="3">
        <location>
            <begin position="213"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="369"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF21413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8145259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="403" mass="47886" checksum="64813E348E4ECD6C" modified="2022-10-12" version="1">MPLIIPEFFIEQDEKYIIIKLKLPYVKPTKSEFNMMGKQFSFFLKPYLLQLTFNEFLKDAEQPAKAVYDPEIWSLKVYLEKLNIGEHFTELDMIGLLMNKKSKKNKQFKGQLIEVIESTNDKQESQNIDQNQDEQQEGYVNPYCYGFNNQYKDVFDDLEEELYEIAILDPKQIAKQDRKSLAFQFIQKQFDKDTYLYDLLENEQVDEILIYEFKFQQQFNIDEINQISKLGKREYLIENKEYCLFGIIDILFAFLYENSLFQGDLNLESAITINQLSSQFQAFYIENDLENVLINNYQRALTFPMIRSFEICVKTKNLLIEVLQNKIQIIKILLAVEGLFAKAKPRNYLNLIYLQDYIVWAQSIKEEDLQTLSKQIKKLKIDKSQLQLNIPIIENEAIQQFQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T173</accession>
    <name>A0A8S1T173_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">YTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0130399</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T173"/>
    <dbReference type="OrthoDB" id="306690at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061157">
        <property type="term" value="P:mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007275">
        <property type="entry name" value="YTH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045168">
        <property type="entry name" value="YTH_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357:SF89">
        <property type="entry name" value="YTH DOMAIN-CONTAINING FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357">
        <property type="entry name" value="YTH YT521-B HOMOLOGY DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04146">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50882">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YTH" evidence="1">
        <location>
            <begin position="318"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8144784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="505" mass="61302" checksum="22570757544C7CDB" modified="2022-10-12" version="1">MVPKLSQLLENNKIHYKTFLQYYMDNQIWSYADKQSNSQENDETSEQKRSLKGYKQYNQNVQNQQAFFNSQEEDLPLRGYKQQQKNYQVSQNSQDQDLPLRRQNQQNKLYYNYQARLNSQEEQLLARRQEQQYQDYQECFIQKDEDLPLKGYKHTQGHLNHQQEDLPLKDYKQYNKKPQSNKTQYNYQEKMPIKQSNSEQIRNQNQQYYYYQDNSGQGCYSDKNQYFRQNPEEQNREQYNHRDNQRSDYYNRQNNQANQQVEKQNSRKQMPQQYNNICYQFQTQFLRANNYYDINELRTNFKSLRQLNKNHQPSQNSHFVLIRPKNGFQNIHKAIKYGIWCSTSSVNQELSSLYYEKDKCVYLIFYPLGQTSQLIGIAQMVSDYDPSQTYKYWDNDGIYKGSFELIWHSIKNVNPQCIQQIYYNVGIGDIKFFNIDQLRDGTQISYYDALQIFEIFNQAPVKPSVFDHFDVLDMQEDQKRSYKLKNQEKEEFQLKQKGLKKKYQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T192</accession>
    <name>A0A8S1T192_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0130226</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T192"/>
    <dbReference type="OrthoDB" id="10335915at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8144432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="166" mass="19576" checksum="40E1869794B27D36" modified="2022-10-12" version="1">MNNLKYQTVGTIINANILKRKLLKRYQITEIDPLQKPYKQRALKIKAKNYFYLVSVATYTLIMIDIKYIKKQQYLIFLSNFLVIIRTPKIKAPESAKEIIKKICYPSIKYIVKKQKLKSIMTISMMRKQCNLYLLISISYKSSQSQFANLKVHRLNSNLFIIITTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T6V9</accession>
    <name>A0A8S1T6V9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0180346</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2443807at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050349">
        <property type="entry name" value="WD_LIS1/nudF_dynein_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44129">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="NACHT" evidence="5">
        <location>
            <begin position="1273"/>
            <end position="1446"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2181"/>
            <end position="2222"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2231"/>
            <end position="2265"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2267"/>
            <end position="2303"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2310"/>
            <end position="2351"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2596"/>
            <end position="2627"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="817"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1108"/>
            <end position="1203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8148995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2920" mass="342992" checksum="E6566B88E011F336" modified="2022-10-12" version="1">MFLQLRGGGCGNSANLVQPKITPINTTEEKQIIPNLQNKIKWHSQRIAQNAVTAIDKKHDLMNSFQFFQIHQRQLWEQAKNPDLSKENIDLILECLSLLLPALKSFIQSNHLYALFTSQSISSLIWVVFTFFSTTKERYLQVQTQQQYLNEIEQIYERLEIESGNETSKYQNNIEYELFLIRAILFVTPTNSQEGVEIFTQFLGGAFQALVKFSLNDDLIQSLKRGVIFLYQQGVKYSRVKKLEIIFSLLSLKFDAMNILEQSRESKQQNNKSIEIVELLGKMYCNVVKESSDWEIWQCWIQTISSLFQFKPVIQKLNLEKSLLDEIQKKDYAIKTLGDQQILSLKYSYIQNDKTSKTLFDSSTILQELAQLQIFILQGYGQLSNYEQQYFLKLQALEEQKQNNQPQLPITLSGISQLLNQVESLQSIFIEFNEIVKSQDFKDQQKLKQSEKLMKQSQLKIIALLSDIENLNTLKMIVKDLSQPKDNQNVFFEDYYQIQQTNCQIYLSGLVNMGNDQFQEIFQFNQYFSIIVELITGSQNGQQFKKMYEQFQKNQKDKSIIINLSNALKNLVFKEESSNFQQNDSLIGFIKAINLITQWKSNLKWNLQISIILQIHLRMLVQEQYYDKDPNLYSQIISITNQHISYQTLSQSIQFLYNQYSQCECYNKFSNLELKSTSHLEKLLNTLLIDNFDAKNTTLSILRKYLFSNSSSVQNVKFDLEISKMMEEELDHSLNYINQIQSALKMIIDFLLYEKEVNNEVTDELEYLLDLKCITEVKQNFLENQTNKYKIQECLQKLQNYFNKFTVIFTNSQTTIKENDQKTYEQLNQKSQTLSQLIVFLTGLSELNIFNKKDEELVEQIKQDIREKLDEIIDYKNIKSINLSIKVNNKIQVEIEFYQKINSQLEELRSKQEKQFNQVKEIQKELQQIQLQLKNIVTLNKLKEQKKSYDTILRETEMDIMQCFNRLKKEQTSLELDFSKIKQQLSEKKNIQHKKDIESQLHFILVQIESKEKQYNYLEQQYDKIKEEIVDFLFSNKWRIRQVIIYELQQMRQFCLSQNSINLSSGLLIKFSVFETDKKIRVLFNNQKGQESQMILNKYWPNQEQMIQNKIKEKIKELNDIAQKLTIETSNQLKIKYQKEYSRLEKEIQGILENVENIGNQLGITILFFQDLKQDLLRIENQIKQIQEAMESINKDLKFLKGRSVKELFEMRMKRVLQQRLVYNSDNVYIQILTKEKLQQEDKEDNETVLFTEDLFGNGEINEFIWKQQKDSLLIHGQAGSGKSTAARKIEEFLWLIYQKNKNQTDYIPLIPIFVSLPQLKDPIYCAIEETLRSDNYRFSERQIEELKEALELKQYRLIIIMDSYDELKQQYIGLNLNISNRISKWRCSSDRNKYPKVITTSRSELFTIKGYSSWFLSESNSQNLYKEVRLLKFTENQIKQYIQEYTFLCVKRVIKEFYFSAYQDQDYQEFENVYVDIIKTVGLLNLKQDQQQMLSSDIIDSLILKCKHFVSTEHQKTMTQMLMEIWSSTKYENFIKLMGLEQVIETPFMVEIVMAVLPYVVKQRQEINNIKENFIKKYVYLSRQDDSVQKQALDEWQGILNNQQFITEFVQEFQIAEQEKKILQYFQNNQRVNIIMKALLLEPLSTYDFYIQFLEHYFKRQINKLRETGEQIDFDSIGNELWEFAHKLANEMTFSNLSQVPFQPSGLVFKKEDKDWRDDFFNDDCQEGTFKRLFRKCIPIKQKSGIYSFNHKSLQEFLVAKWFIEQLIKLDVQVQETSINIDKAQQKQLVKYNFFQKSWDFDYMQGPIRFIINKIKFNEELKQKLMNLVFYSRFDDGYIIGSSNSLYLLNLLGQQFIETDFQQIKIKQVSLINANFFGCNFNGSTFNKVKLSGVNLNNAEIKNAKWQDILIDELPKIETNLSLVSQIVYIEEENIFLVHDNISVKQYNLYKFQEEKKLNIDFKPRFMVLSNNQKLLAMMNKNEVLIFDIMNNKAIQKLNFGDCFSQYKETIIFGPDDQSIILGGSDGADELRLIIQYENVAEIKNSNIRSKHATKANQKVKMIEDDNDDNDLIQTNNIQQSQKKKMIKVQPDSIVAPDEFHSLTSEKVYELKCAKVVVLQHFFNFSIYNQKDKKLETHESNLKKLTCSDINRDSTLIAFGGEQGELIVFQISSIDINFGLVGHKEYISQIQFSTDGKQLVSCSWDKTIRLWNIQQKSLISQTAFILKPKVYSLCLVNNSALALTGFNDGLIQLWDLENSDSRMDVNKGHQAEITCAIFSPDGNYIISGSVDKMIKIWNTKSGIQEGQNLVKHNQTILSLAISDDSYLLCSGSLDGYVYLWDFKSQKFNKQINLYGSQVNDIKIIKYNNEYRILTQTNESVVQLWQESLQEYYLLYDYVDVNKRSISAQESSEILLQSKNSNTFVREFRSITYDALKDHKITSIGASIDGLIKLVGTDKGTLLIIKPDSRSEQKLTQLSQPIQMIKTLNSKYFLTVIQQNIFIWWFSDFTIKESILTENRVINDILVIEDQFICAGNLIEIWTISNQFKVQKKIFIQNATITAIGYDQKNNKIYAGLYNGNIAVIDQVTDEQIQIEQAHSKEVKTVHWLKTRNLLLTGGEDLSLKIWGSDLGLIKEVEIYDNLQQTFISSNEIYMIVQESHQISIWDLQQIELVENMIKIQKQAKVMLINSTQQVFWTNEQQISMLPIMNKKIKSSYMMEKQSHDPEFMATTEKMIITADRKGNLFMWDYDGEKIDQKRTDKQNELKSINLSYDQTKLVLAFKNLITVIDLQKKQDIFNKDFQNFEITRALYLNNNDLIIVTNSDKSQVIKFSVLDSKKEIIISNHIGKTLGAATYYQSYLTYGSDQAIRYYVEQQSCKFVFSSQLQFECEGAQIRNSEISFKSRIDLAVLFKQKKAILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T914</accession>
    <name>A0A8S1T914_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0160199</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T914"/>
    <dbReference type="OrthoDB" id="637682at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003690">
        <property type="entry name" value="MTERF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068">
        <property type="entry name" value="CGI-12 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068:SF151">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR MTERF9, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02536">
        <property type="entry name" value="mTERF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00733">
        <property type="entry name" value="Mterf"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8147082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="327" mass="38983" checksum="25F976707041523C" modified="2022-10-12" version="1">MRAIFFRFSTYSKFTEKLQNTKFATIYNYETQIKPALSIYESMGFNEQMIFRIFKNRGLSLVQGNNVNDIGQLNELFKTRLNAETKQIRTILFKDPQILSHSIDKMNNYIDLFEKQLQISKEDILSMLTAYPLLMRNFEVNYEKFRNVFNTYAHINDKQFGQVLVNTPFLFSFNLERIPPNFRVMYNREYKTKEIQELIQKTSEFLALKNHDLDRLLNHYDVLIPNKEVQHQLLINNHKLLLLTPAYMLSPKINLFKEIGLSINQIGQILQICPNLFLKSVQTLKLKLKFFEKHLNVKIKDSPYFPYILEFDYWTILRPRVGDFKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TAE6</accession>
    <name>A0A8S1TAE6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0170021</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TAE6"/>
    <dbReference type="OrthoDB" id="284045at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8147552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="605" mass="71388" checksum="949C58793E6731D6" modified="2022-10-12" version="1">MIKRKISKFTPFDRVLDGLEQEELIDEDVNMKIIKVYNRKKNNKMFLLERLISNEKEHLKYIDEIHKYEIINADGLLCIDKWVFIIYQQTYDMLIDQNLNKLYKFILVFDTYSQNLNQYLENIQSKKIQLLKEDIVVFFRAIILSINYLQQQQIIAFNPVTMSCILICSDSCMKFNCIPILLTQHALEKAKKESLLYQAPEQVKDIKNINHIYSQAVYSLGLIFIQLFLLKHIDQLRKYEDIKQSIKEIQIKYGDAYKSYLESMVEQDPHKRIKLQELCQTLSIQEPQKQIKDNIKITFSETFEYYRKQYSILNVPEPRSIAYGIPWSRNIIQSNLFNMKNYFLELLDLSQSQQISEQAVTIIFQQFRVFVIGGMNTHLVYELNDDIPALKEVTSVPQSNANRWGFGYVIQNNQLLYICSGYSDQLLTANTFCYNIQTQKWTKLSDCQKPGIGCTLVCYNNMSIFKYGGTEINNKPLNCVEEFKNNEWQLINFKKPHFILPNFSFAQQVNPTQIFIVGGYFDKEHNTKVIVMNIDTQAHLIDSRKDYTYVEFVVNEFLDYGKYGPVEAMQISNNQLFLLQKKNEKHQYLTVQDQSVFVELRKIHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TD37</accession>
    <name>A0A8S1TD37_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ras-related protein Rab-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Small GTP-binding protein rab1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0190286</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Protein transport. Probably involved in vesicular traffic from ER to Golgi.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TD37"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001018">
        <property type="entry name" value="Rab family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0636">Prenylation</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00053444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00067099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00081865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8149778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="210" mass="23931" checksum="A665E18EDE758491" modified="2022-10-12" version="1">MSLGYYDWLVKVIVLGDSGVGKTNILTQFCDSKFSQNYTATIGVDFKIKILNVQDKKIKLQIWDTAGQERFRNITQTYYKGAYGIIFVYSLIDRNSFNNVEGWIKSILESTTDEVCSILVGNKLDSQDRRVQTAEGEKLAQKYNMPFIETSALENKNIQDIFDRLGQNLKIRLENEGQKVSNNIHTQSGQFKLNQEVNQTEQPTSSCNSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TE25</accession>
    <name>A0A8S1TE25_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0200104</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TE25"/>
    <dbReference type="OrthoDB" id="284453at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8150128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="105" mass="11988" checksum="2BEBBAB13E34F0FE" modified="2022-10-12" version="1">MKIEVHIKDKQFAINCGDGLQKVKWLGDVAIFRHSHFYSQINSTTKGIKLENGEMVDLNAIISSTLAENTHVWVVLKEDLEAMGMDNKQQQNQHKRPITAISRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TE58</accession>
    <name>A0A8S1TE58_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0220375</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8152291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TE58"/>
    <dbReference type="OrthoDB" id="296667at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053238">
        <property type="entry name" value="RING-H2_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14155:SF627">
        <property type="entry name" value="OS06G0192800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14155">
        <property type="entry name" value="RING FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="328"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="156"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="408"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="498"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="500"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="160"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="180"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8152291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="535" mass="61730" checksum="560A0B5B9F34E02E" modified="2022-10-12" version="1">MIFVLLLLISQTNALYSKKLYRTRGFTAIDIDLQKYDELNQGSTTINIILENNSFSTVPFLAYCNTKNQQIIKSIVSSPEAIKKQIESNKKLCVFDNNALLYSQRVQQIVLSDQINDENEWAFNKFSIYKLNEKQISIYVYSSDDSQYTIIIKGSQKGECYNQCNNYGLCMSNQILFCQCNEGYLDSTCQLQSTVVNAPSLVHFEMKEQSYKNILTIPLKATLASLEIEVNTNARIKTYLGCQHDKDFIPFYESNSFYQTEISTGTLKFSKDDIQYCLDNTNKIEQTLGFRMENYVILLLVNDQTYKIQVSLKLYIDSKQEDNSGLEIYYILAGALGALIVALLIIICVIYFQRKTRRINNGVQNNLNLRSFSVKGNTNRSLRQDYIPVELYEQIIQEYPGLVEISDCQICLVEFEKQDLVKLTYCLHLFHSTCIDEWRKRNHTCPFCREDLTKQKCIQQKQEDQIYQLGVISGDAMQIDEQKLAEFQRREQRLRHLQQTSSPDSANAIQQKENTPSPFLKGDVITQQLCVSSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TG79</accession>
    <name>A0A8S1TG79_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0190355</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TG79"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8149869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="139" mass="16606" checksum="0D5A625236165A32" modified="2022-10-12" version="1">MFLCQNLEHEGSSIIAFCLSADCQEPKSQFSFNCLINPQKHQNCRIDCKPFDKIDTLLDLILYKLDKMLEQTDQKFELLKLEYEKTIKIIQQEQQRFYEIIKNLKEQKYQEFLDNIGKIKNWNTIMKSTQFEKELLISG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TGS9</accession>
    <name>A0A8S1TGS9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0200249</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TGS9"/>
    <dbReference type="OrthoDB" id="203440at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237:SF9">
        <property type="entry name" value="NEUROGUIDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237">
        <property type="entry name" value="SOMETHING ABOUT SILENCING PROTEIN 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8150416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="299" mass="35494" checksum="52DAF5A6C18DD1F8" modified="2022-10-12" version="1">MSGGLTYFNEDFPVYSELISTINDQLQQIDNLHVITCTTENKNNIAQYLRAKNATMTKYLIHLNMYLLHKLNYGNLDEFPVDQLIEDKILLQKIIQAQKKLQYSIDKLSKYQETQINEDDQIVDQVEKLTLKPRIENLHKDENNEKASNPNQKYVPPKLAATLSKNDLNKQKQERREELRKTQKKANLIRSILDDQETDKPKEMTERDLQQLYYGRAEDEKQIEKRKYEEDHMTRLPTTRDEKRRERIAERKANVTRLDDFQEFETINKVLNKGTKKIKKPLGSKLKGSMKKILKRKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1THD1</accession>
    <name>A0A8S1THD1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutamine synthetase</fullName>
            <ecNumber evidence="3">6.3.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0230142</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-glutamate + NH4(+) + ATP = L-glutamine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16169"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glutamine synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1THD1"/>
    <dbReference type="OrthoDB" id="1936100at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000011">
        <property type="entry name" value="Glutamine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050292">
        <property type="entry name" value="Glutamine_Synthetase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20852">
        <property type="entry name" value="GLUTAMINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20852:SF57">
        <property type="entry name" value="GLUTAMINE SYNTHETASE 2 CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51986">
        <property type="entry name" value="GS_BETA_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GS beta-grasp" evidence="10">
        <location>
            <begin position="23"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="GS catalytic" evidence="11">
        <location>
            <begin position="111"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8152615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="380" mass="43435" checksum="7D997A35C5E160F7" modified="2022-10-12" version="1">MTEQLENLRNFNHYELNSNVKVTLAEYIWIDGTGLSLRSKTKVFHHEIKQLSDLEWWTYDGSSTEQANTKWSEIYLKPVVYVRDPFRGAPHLLVLCETYLPDKKTPARYNFRWLAQEIMEKAKEFNPWFGIEQEYYLLKRTGTTHTWPLGWPNGGFPYPQGRYYCSIGERNNFGRALAEAHQRACLNAGLKLAGINAEAAPSQYEFQIGIAQGIEAGDHLWLARYILERIGEEFGIDINYDPKPIKGNWSGSGAHFNYSDSKTRGEGGYQYILKTLIPELEKYHEQSLFLYGSNNIDRLTGAHETSEYGKFTWGDGSRGGSVRIPIITKELGQGYFEDRRPAANIDPYLVGSVLVDITLLGGTYLEKLKQELFKSSQPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TNK5</accession>
    <name>A0A8S1TNK5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Insertion element IS150 protein InsJ-like helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0230215</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TNK5"/>
    <dbReference type="OrthoDB" id="304160at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055247">
        <property type="entry name" value="InsJ-like_HTH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13518">
        <property type="entry name" value="HTH_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Insertion element IS150 protein InsJ-like helix-turn-helix" evidence="3">
        <location>
            <begin position="76"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="167"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8152736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="237" mass="27641" checksum="F3A5A1F912C6B795" modified="2022-10-12" version="1">MTSIEQLSKVEGPQQILQADSSTSSHGSDSSDSSYDFAQDLNQKPSFKRTPEEEKELLYRYRGKHGLKYSKISNDQRQRLIKQVTTTGCTIKSAAKELNINFSTAKAIMQIYRKEGRISKKIKRENKKTLTKKQEILNQTTSDKPNYGEQRQTKVDVVQDNLETNLYQQAEETNRSQALLIQQLNTQNLLLSDRVQQLQQENQQLTQNYTYLSYQYNQLQQMMSQMMPSYYRPYPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TWT5</accession>
    <name>A0A8S1TWT5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0290114</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TWT5"/>
    <dbReference type="OrthoDB" id="286249at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8156678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="125" mass="14630" checksum="17B9FE9EF491CF20" modified="2022-10-12" version="1">MSTKVRISKRGQQYFVSQKENYTPFSNLSLCPKPIIALAQSSYDPNLIESTLNQLKIPSQLKHESHLNRMKQIADKQEYYVHGQITSCSQIETIGQYSDHSYYDQLEANHTKNQQNYFEDSYQLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U0A3</accession>
    <name>A0A8S1U0A3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0300109</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000030">
        <property type="protein sequence ID" value="CAD8157353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U0A3"/>
    <dbReference type="OrthoDB" id="308323at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8157353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="117" mass="13355" checksum="054856348C9E116F" modified="2022-10-12" version="1">MYKLISNFAKSLSTQAPIYSYHFPNGSVYNNPAVTAKRIIKVVGERLRKIDPERWESTPITFNTNWNDAAGYVDVATCIHIHDALEKEFGIEVKDRAFLVSSIETAFYIVNIHHDSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U1N4</accession>
    <name>A0A8S1U1N4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0310054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8157892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U1N4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="11"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8157892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="123" mass="14412" checksum="B6971644FAB570C9" modified="2022-10-12" version="1">MDQVKAMQQYITKLNIALTDLQQKNKDLEVKLDQEQKLKNQNILKKKLSNEKEQVEILEIKVDEYEKTLKSLSDTKKPIFLGKVFNNSQLDQLIIKLNQSEQSLDLQILENEKLHAQVFTLKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U1Z1</accession>
    <name>A0A8S1U1Z1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0310342</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U1Z1"/>
    <dbReference type="OrthoDB" id="5979581at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001190">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="17"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8158444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="423" mass="49681" checksum="F934AEFE3E6F3C63" modified="2022-10-12" version="1">MDSPLKDMTGTIVNKQYKLIKKLGAGAFGEIYSSQSNGLEYAIKIEKSDSKHPQLEFESKLYHYLNNHNGQGIPKYYGYYQQDGYNFLVMELLGQSLEDLFSENNRIFTLQTVCVLGIQMLECIEFLHSKQFIHRDIKPDNFLMGKAQKDRVYLVDYGLAKRYISKDLHIPYKDNKALTGTARYASINTHLGIEQSRRDDLESLAYVLMYFLRGSLPWQNLRANNQKEKYDRIMEKKLATSSETLCKNYPQQLLEFVDYTKNLKFDEKPDYQFIKNLFIQIMQENELRMEYIYDWDDEDTQRDKIQIRNDEKNNLLKTNKQSSNTQQRVNKYQENKNINSNSIYMRNCSNINNKYSIGRTKTNSIGSINKIVVTKTKQITNNTINSLTNQNTLPNNNNNNNYNKLHQNLAKQIIKKHSSLHYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2023-02-22" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U6Q0</accession>
    <name>A0A8S1U6Q0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0340266</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8160298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="15"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8160298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="295" mass="34437" checksum="D7CE9262C7EEB203" modified="2022-10-12" version="1">MLRVQFKWVKLRLVINKLKQVINKLRLAINKLEQVIKQLRLVINKLKQVIKQLRLVIXQLENNLHELKTDEQGNTEINNLSQGYSYTVLIYKSGFKKSCSVLDLQRRVPKTKYIFRLTKSIPKHSVRILLEWTSKTLNLDLYSAFKVGEHSCLTGALSKSCGGMQLSTISKNDQHIEVLDIYQLEPQIYTIFVKRFLTRNEALDLLKNSLVSQEWIDADPHITVYLSELNYPLIEFRLPQNINLQMDKLDLTWMVFKIDGQQSDAPQTLQKMESKIANDEIRQNTASNKPFWPEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UB45</accession>
    <name>A0A8S1UB45_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA polymerase beta thumb domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0350239</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000035">
        <property type="protein sequence ID" value="CAD8160922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UB45"/>
    <dbReference type="OrthoDB" id="205514at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029398">
        <property type="entry name" value="PolB_thumb"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14791">
        <property type="entry name" value="DNA_pol_B_thumb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA polymerase beta thumb" evidence="1">
        <location>
            <begin position="218"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8160922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="281" mass="33646" checksum="935CE8A8BA095F88" modified="2022-10-12" version="1">MKKIKRKLYSIYKKAVGFLVKALPYPIKYCEDLKNMPKIGDKKIIEIMQTCKLAKKKMLPFVNQVEFLVLDQQVLLPSILKQLKLLGIRKRIEICQIEINKFVCIQWKIQNKGFEEKKLPQYIRLFKKKSMNYVEYKDYIRQLYIDLIEEKKKHLERWIFQLQGVMRGNLLNHHLKMPNRTHHDCKTYMDIEKVSNQAMLRRIDIQNCIQKNSMVVQFCTSLHEIVGSKNGYILSDYELYPNQKDAYKKKLWKGEVTLCKEEMDVYKILGLLYKPTKERSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UBE2</accession>
    <name>A0A8S1UBE2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0370234</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8162168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UBE2"/>
    <dbReference type="OrthoDB" id="286453at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8162168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="120" mass="14838" checksum="FED79A27C0EDE5AD" modified="2022-10-12" version="1">MEYYTEISNELKNKYNQHYNLYQKQQLERKILCYKNNNEDPLKYQQCIDDLNTRMNMNSTTLRNRYNQIEIDDKDCQSKCYEDNKCFKACEDQSRKKANQLQEQFYKLMLQENPEYKKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UBV1</accession>
    <name>A0A8S1UBV1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0360281</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000036">
        <property type="protein sequence ID" value="CAD8161627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UBV1"/>
    <dbReference type="OrthoDB" id="307159at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8161627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="299" mass="35313" checksum="42A5FF57B7BD1696" modified="2022-10-12" version="1">MKIQRRSLAKEIKAQRPLFFNQSGVSPICTKLYSQQLKDLKNVESYVKLRSIKCDKSISLLNCSKQICRIDESMPQVKCNTQTRINNPNESRILTRQVIKDCQEKRKEKYQSLDESFKNLYLFQDKYSEKYQNYLNKHIEDSIQLKESISFNLDRLSKQQLYLKLKDLNKNLNVNSKQEYCDPQKFNLMEITLRSTVEPSITDISPLTICDDTDNNKMKDKLKLLVSPQNFEQNHRKRQLTLPYFSKNQLNLRLNQINDGNSDSQNLTSTQTSNINSLMRNKSYLYSREIKRIHIQTEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UEP5</accession>
    <name>A0A8S1UEP5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0400033</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8163731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UEP5"/>
    <dbReference type="OrthoDB" id="289913at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045111">
        <property type="entry name" value="Vps41/Vps8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616:SF8">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 8 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8163731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="581" mass="68988" checksum="59FC393F3B4C5559" modified="2022-10-12" version="1">MISDDIYWVPQGVKVDCTIDDLIQPNVTNFATFQFNFDQIKQPIEEKKQKNIEDEILELLGITEEDLLLESALQTYDHKLYIEELIQEALQDKQQQIQFTPQQKQNPFNSSFKTLYERQYLSYSDSTFNFEEEINFKTPDLATLKMLLEQYCNDKEDLEDLEQYLTNNQQSKIIHKFTQLRGQARQLVFNYIDLQQKDNQFIDILSQKLKELSLINPVKTEYLLDYFNQEQLRNILDKQIPIVRFKILQSLQKDDKLMIEYFELICQLHPDQVITELQKGGYPQDECLKLCRFYGNLKGLAYLLERSGSVLEAINLQFDLFIQGLKQQLQKNPQLLEGQKDMYAYIQESLEPTLNICRANTKRNDDESDNNWFQVLIRLTKLRQEFYKIRFFPALRCFNLHFTRLLEEVLEKTKIKSLLENLNETMKYFEFQELKSTFSQLLSGQLYELAIYGQSTMLIKQTCNKYLSSLFKQSQQGISVDIYCTYCQKLIEMTSKVYIDCKHTFHIECSSEQLCKACMNNFGILINKLLTLYTNRKSSRFQVPIQTVESNQQKIEDTNQRQNKINKLKEFDYLRQNNKGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UGW6</accession>
    <name>A0A8S1UGW6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">cyclin-dependent kinase</fullName>
            <ecNumber evidence="1">2.7.11.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0400212</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8164080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UGW6"/>
    <dbReference type="OrthoDB" id="298079at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07833">
        <property type="entry name" value="STKc_CDKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000049">
        <property type="entry name" value="cyclin-dependent kinase-like 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001072">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="4"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="407"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="416"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8164080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="470" mass="54863" checksum="DF34094303877D2D" modified="2022-10-12" version="1">MNKYEIMGVVGEGAYGIVLKCRNKETNECVAIKKFKETEDDEAVKKSIQREVKMLRMLKHPNIIQLKEAFKKKGKIFLVFQFVDRNLLELLEERKTLDQNSIKRVILQLVLAVHTCHSVGIAHRDIKPENLLIDNELNLKLCDFGFARTIQSQEQLTDYVATRWYRSPELLISNNYGKQVDIWAIGCIMGELIDGQPLFPGENEMDQLYLIQKIIGPLTQDQMEKFQKNQRYIGMKFPEIVKSETIEKRYSGKMCNKGLNFLKECLIMDPNKRMTSLDCLEHPYLSDVWNKESDVRPKNNFQRRISCERDDSKHSIINQYDFGEQKTKKIQEKAIPQTIQNQSFVQRQVYNYNIGDETTEGNKEGDDTKSQMHKTFNQSFKIKFNDTLPQSRLGSEQDKRINKVIGLPGLKEDSKQQQNIHNKSPNKRTSIQQPSFYVQGQYNLQPQKLNLVYNANTYNYSIKKSYISKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UGW9</accession>
    <name>A0A8S1UGW9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AMP-activated protein kinase glycogen-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0390299</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UGW9"/>
    <dbReference type="OrthoDB" id="312936at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-activated protein kinase glycogen-binding" evidence="1">
        <location>
            <begin position="173"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8163594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="234" mass="28024" checksum="79D66BD610CD631E" modified="2022-10-12" version="1">MGNLCLRKKERSQQPQYNIADENHIIYTPASLNELDKIIKIESQEALILNIQNKQDQFQYENNQNTIQILNELDKDALIKQGQLAKEELSKIKQQQYDDAVLYFGKQSLKENLPIKSEQNDEQSVQIELERIRGQQYIQQQRDQQQEHQKFIQKQEVEKLRLQKYEQEIIQIEVKVLRSWLNWIDKIELLQKDNRFEIEVNIPTGNHQFKFIVDDEWKVSDQNEYNGQNNLINV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UT62</accession>
    <name>A0A8S1UT62_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0470239</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000047">
        <property type="protein sequence ID" value="CAD8167674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UT62"/>
    <dbReference type="OrthoDB" id="289314at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555:SF170">
        <property type="entry name" value="FI18122P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="3">
        <location>
            <begin position="133"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="392"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="10"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="39"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="81"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8167674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="515" mass="60535" checksum="138711A7D242072F" modified="2022-10-12" version="1">MSEDTVPEKQQVLQESTQEQAQQTQIEGQNQQEQQVKQDTQKKVENQENQEKQEIATEQVKQDKEDIKEKQQSSQTQEQIIQKDVESEITKNENNIEENQVKEQQEKQQITEKGEVIITQNLDNLFLKADYSFPIEIKVSDPVQKGGNISTYYVYTIKGKDCFGEFETQRRFNEFYTIRELLLVKWPGQYIPPIPEKSVSTGSEIIIERTRLLNIFCMKMMQIKHLYYSEEFYDIFLRSTNPDLNKQLAQIPKQNVFAYTDRYKQTFQITEIKEISPEMIKKMSGVQSFLKQSQISLTKQLEQSKSLSSSRKQLKETFFTIFGLNISEYEKQILQEWVPQNKLIFANPANTDILELTKKIRDPCNNSLDKIQDLISTEVRDIEAFLQAFQTRDSLMQSKQKAEQKLSEEQSDLYKIMKGNSTMKGSFSKLSLEEQKSKLELQLNETQKEVDQYKLLFQLVTDVLTSYTIDKFKKDKHQTYKSILADLAQCEQQYLEIQQQFWSKVQQVITQIQEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UV71</accession>
    <name>A0A8S1UV71_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Translation initiation factor eIF-2B subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0440102</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000044">
        <property type="protein sequence ID" value="CAD8166346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UV71"/>
    <dbReference type="OrthoDB" id="269919at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10470:FF:000054">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051855">
        <property type="entry name" value="eIF2B_beta_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45859">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45859:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8166346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="338" mass="38274" checksum="55C3D341BD10D7F9" modified="2022-10-12" version="1">MSTLKAQPRLYSSRESILTLKAHLSGKQAKIPKNPTMEDYITYIKKLSNRLKQGPLDFWIPNVFRRLIQVIRLMSSEDGFFPIKDKEKNFKELGLTILRKSIRSKTTVETGSETGSNAIQQDVILPSIEQVLEELEAHSDDINTFASTHFFTNEVLLVYEYSTTVLNFLLSAKKTRNFEIIVLESETENLGKQFATDLGKHNLNVTLTPFTNAYAIMQRVNKTLLGVDAILKNGGLLMHPGTYAICVLAKQFAVPVIVLSGAHKLTPKYAFDQTTFNQLVSPLKINPNSTIDHMSIGITFDYVPPEYISLYITNQGQYTPQSIYQLFSDFYNVKDEDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V530</accession>
    <name>A0A8S1V530_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0540021</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000054">
        <property type="protein sequence ID" value="CAD8170919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V530"/>
    <dbReference type="OrthoDB" id="306004at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF274">
        <property type="entry name" value="MYB-LIKE DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="65"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="128"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="157"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="191"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="191"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8170919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="478" mass="56558" checksum="FDC7561B0DACDD00" modified="2022-10-12" version="1">MQQIDRQLMDDFHQNFLNFFAKVKGVMNVRFNYMREKFNLKIDTFPDDQEVFNIQQTNKLNLQTQPNKLKKNWTDDDKKVLIWLVGKWFTLNKRDLKQLNDTDWSSIASMMPRRDAFKCKQKWLQMLKLPLQQAPWTFDEDNLLQKIIQDFQSQNKGNKWSQIATALNKANDQQVHRNGKQCRERWNNHLNPNINRNPWQLSEDLDLMCQAKEIGKKWALISKKLKVARSENNVKNRFNCLLRKEKCHKSGKKDEDENQSEESSISNLAQTEELTHEEIKLINIIIKKIEWRIQQSDNIQQKEQNDEVKEEHQDIVKKVRIESQGTFYKDFKQKISNFKNIEKLQLQVKESQLNDDEVATLTPCLINKENNQIYFASPEQLYIYLTKSTETNQVGIQQENYLSNPNSLGSVMYDPKFFRSIVMLQSNEIHQQQSGIYNQRSFVNQNHMGNFQSLNSIPYSVALSPYPSQVNLANQLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V5H5</accession>
    <name>A0A8S1V5H5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0570171</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V5H5"/>
    <dbReference type="OrthoDB" id="10291377at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="274"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8172590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="692" mass="81966" checksum="F15A481404E851A1" modified="2022-10-12" version="1">MNEQQRFIKLEESIEQLSKSILPHINQISKMQQKLSALDVFITNNPRVDLISDTIQILNSQLKIQERLSSIESQHSQFSSHYDEILNKHSVCIMQIQPWIDEMQSILEDAKQNREMNNQMRINFKEMNQKYKEYTGILLQNDRLTQNYKTRLDQFESLLASKIDHSEIKKVQDLMRRDFVTCIESDNIKNQIQQRIDRLQGEMCTKQQLGIAQDNIQFIKDRQVQVLSELVDKKIEDLANNKLKVYSTKNDQRVYEDRVNQVCKILSEKILMMQKEIQEECKLMQEQIKDENQQLNDLKQQLNVVNSTFQKVVMKDELQISMQKTIRLQTQFEYLNEKLESISAAIFEQKVSFQQFNKQKTLEIQNLQHQLIQKEGESINYCESPKKTEIYQEEMQSIQSSTQKINKQSSNLYRDGSFINSDIMQRIFQRSDAKIQELQNQLDEFRQKLPKTYKFQENSTQVSDTINIRMEYFNQMIIQLIDESKKFKSQFELLNLQIIAINNKPQIKQEKLQEIDDKLKFIQELEVKLDQKCDSSLVIPILDKKANAQEYMTTKLLTKQLKQCVIDTINSLRTYFASDQNDQIKDLLINQLNRYLCYFARSVEVQQQNQNDSQQSTQQIKCLQSKYTAQRMKSYSSCSKRRETDCDIRGRTVNRNITETVLYNSLRSSPLAFRLVNQNKQSTKIVKRMKVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V902</accession>
    <name>A0A8S1V902_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0580197</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000058">
        <property type="protein sequence ID" value="CAD8173065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V902"/>
    <dbReference type="OrthoDB" id="674604at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="271"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="321"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="365"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="408"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="187"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="198"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8173065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="542" mass="63002" checksum="C336FFB169833816" modified="2022-10-12" version="1">MIKLEEKASKILCSQHQKSIQYITLSKSSNNRLLCQKCPQNNRIAINKASELLMDINQDHPLNYHIKELQKQYESLRKEIKLKVDELLDETEDCLNSLINHKLSDKQIQHYLYDDKLNEQDFEKLQEILCKYYDFEQDNQIVAKIPSNDMHNVYQVQQKCQKIASIIKAFYKSQYHSRLGTQQSQENLLSQMPLKKNTKAEKPSEQDKHEEEKSKGLYTIKEEIITNSVCHCIDLNKTATLIVCGLQGNNNEDNLVLLQKKEEGFKEFQTLVGHVGSINAVCFGKINNSIFSAGDDKTIRIWNEYLQSKENKLYNCKQIINEAHNMEIHCLCLNQLNNLLASGCQEGNIKIWIEVDEKWINSQVLIQHSQWVKSLSFNYDSTFLVSGSGDKLICVWNLNEDWEHFQTITEHTNLVNCVNFAQGENIFVSGSEDQTIKIWNQQNEPNGKFICIQQLEGGYYIRSVCFNYNSLLLVSGSYSNIKVWVKNHAGVFVLNQQKYFDSRQVLLSEDSKDLFSVNRFSLNIRMFRIKEKNKKNNAKLTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V917</accession>
    <name>A0A8S1V917_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alpha-galactosidase</fullName>
            <ecNumber evidence="3">3.2.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0600190</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.</text>
            <dbReference type="EC" id="3.2.1.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.22" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDO010000060">
        <property type="protein sequence ID" value="CAD8174028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V917"/>
    <dbReference type="OrthoDB" id="5795902at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004557">
        <property type="term" value="F:alpha-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005995">
        <property type="term" value="P:melibiose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14792">
        <property type="entry name" value="GH27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000202">
        <property type="entry name" value="Alpha-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002241">
        <property type="entry name" value="Glyco_hydro_27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000111">
        <property type="entry name" value="Glyco_hydro_27/36_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041233">
        <property type="entry name" value="Melibiase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11452:SF75">
        <property type="entry name" value="ALPHA-GALACTOSIDASE MEL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11452">
        <property type="entry name" value="ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16499">
        <property type="entry name" value="Melibiase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17801">
        <property type="entry name" value="Melibiase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00512">
        <property type="entry name" value="ALPHA_GALACTOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035899767" description="alpha-galactosidase" evidence="8">
        <location>
            <begin position="23"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha galactosidase C-terminal" evidence="9">
        <location>
            <begin position="302"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8174028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="382" mass="42476" checksum="1EBBE69BF84EB6ED" modified="2022-10-12" version="1">MQKYFFICVILITLNALDNGLGRKPPMGWNPWNKYGCNINEEIVRQTADALVSTGLAAAGYIYLNLDDCWQSARDPVTKKIIADPIKFPSGIPSLVQYVHSKGLKFGLYSDAGMQTCEGKPGSLGYEEIDAETYSEWDIDYLKYDNCHTDGTSPKVRYPPMSAALMKQSKKIYFSMCEWGLEKPWLWAPPIANSWRTTGDISDHWYSFIAILEEQANLAQYAGPGQWNDPDMLEVGNGGMKTHEYQAHFALWAILKAPLLIGCDITNMSQDTKKILMNREVIAVNQDSLGKQATRVKKVLTSEVWAAEVADNGAGVVAVLFNQASIQESITIEFDKLGISGAQNVRDLINQVDLGQSTVSYTCQVPGRSAVMVKFRSVQSEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VCE0</accession>
    <name>A0A8S1VCE0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0610160</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VCE0"/>
    <dbReference type="OrthoDB" id="40048at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8174547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="75" mass="8995" checksum="A19A97E686E4D7C9" modified="2022-10-12" version="1">MKETQIQCQQNEIQQPLAFTFDELSKVLYVIDKESNIIKYEYNNKQLNIQTTYLQIRIINSEQFVNFVFPIFNGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VCL3</accession>
    <name>A0A8S1VCL3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0570199</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000057">
        <property type="protein sequence ID" value="CAD8172646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VCL3"/>
    <dbReference type="OrthoDB" id="306866at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8172646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="160" mass="18613" checksum="A6AE08DA5534126E" modified="2022-10-12" version="1">MCQNSESSECYSKSSGLDNKKQNKKIQENSSQSENDQIEKKVKLNDERGQIKISRKMSDDLIKIFERLPNDQITKLLDVIKVKNSPDLPVQVNLDDLMSKEDDFVLDSQSDSQNGKLRKRRESKEKECKCCKQIVIRHHCIHTECEKPCQFQEKINKSKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VDG6</accession>
    <name>A0A8S1VDG6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0620041</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8174877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VDG6"/>
    <dbReference type="OrthoDB" id="10295043at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="314"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="272"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8174877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="340" mass="39600" checksum="9896936673010DC6" modified="2022-10-12" version="1">MLFSISPQPPFQIHHRKQTTFQVINMVDHPILFRFKNDIHIRIMPSYGMIAPNERKLISVTNKTSQFDIDVLLEAINYVEEYLDMLEYSNPQLWIEKQPGILPTQMMSIRMNINTDSSSTQQSDQKQNFEEVIKQEPLHIHRQQHTFSRFQEQIVHKNSLDGQESRASYISNNNPSISPILQDASRLSANMSREAKQPTFFTEAYEIPQEPNEEFQDRMDPEQSYNNEPYASGNFICNDDENSNHEVSGIKSQTSIAKFEQTQLITSLKQKEQQILKQIDQIKISKDQLSEELEKLKFKAMFKNKENNKQDQQILIGHLLIVSVICLILGALLKKIQQLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VEJ4</accession>
    <name>A0A8S1VEJ4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0620025</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8174845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VEJ4"/>
    <dbReference type="OrthoDB" id="416030at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050930">
        <property type="entry name" value="MFS_Vesicular_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506">
        <property type="entry name" value="GH10249P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506:SF26">
        <property type="entry name" value="MFS-TYPE TRANSPORTER SLC18B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="315"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="340"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="388"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="433"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="42"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="477"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="498"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8174845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="530" mass="58212" checksum="91E7D0567321E576" modified="2022-10-12" version="1">MSSDQQGDQKPLMQPFSVMEPEEKKEQAYNSSKYEGKCVDPRLMTFCITNFVINVGISQIAPFYPGLAADQAGLSYSQIGLVFSINPLGSILFSFVIGSFIQVWGRKKCLMIALAVQSSVQILFGCLNFMTDNKALFFSMSLISRFFQGMSRSVYSTVTFAYVPMFWPGEFQKKLAIMETMTALGLLFGPMIGSVLNYAFGYSIPFFVIAIFFLCAEIPTYTQLPPDSTLKSFEKKKKLPIGKAFTSIKVIVTIMNVMSITAGYTYFNPFFINHMQSFGISENIAAFILTIPAIFYIGMVNIIPKIGKYVKKTFMMSLALMICFSGNMLEAPFWGEGNTLASVIIGLILVGVSQSFSMIPSIPQISEFLAPVVTDPQFKNNLTDMASALFIMSMGCGSLVGPLLGGSVYDGFGGNVNLLGSPPQELIDQEREAFRGAMICLGLWQLVAATLFFFFGDGYKGWSDCCRTCSTGQNPSLDEGQQLTKPLNDDTSDEVMSDNDTLSSEASVGNDNDLLQGPPKKTITIRKEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VFV0</accession>
    <name>A0A8S1VFV0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0620269</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8175293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VFV0"/>
    <dbReference type="OrthoDB" id="305102at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8175293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="265" mass="30405" checksum="5EDEF054DCC102B2" modified="2022-10-12" version="1">MYWNFISKRVLQDLESIPNSTPTHKTINAQTTKINHSFELDSSNQFSTSKIRCQSTHEISEKMVTSKYQRFHDIQIEFPMVRKRRLANILSNPNDYCQRTRPGKKGTLAGTILKKYKDIRLSNSNNDKLLQCSFQTQDVISSRKSINMMQSQKQVIERQSNDSTSPRLPTTMSPNTPTASKKQNKYIIRSKLGQSVDLEKIKPPRQLQPLSDNQSCSPRAIRQVTDNTQHKKTTSSNSTNSLYVMNKVYGNTSNKNLPKSIFKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VHR0</accession>
    <name>A0A8S1VHR0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0620267</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8175289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VHR0"/>
    <dbReference type="OrthoDB" id="312579at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="581"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="475"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="67"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="481"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8175289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="647" mass="75853" checksum="A31D727BCC2AB044" modified="2022-10-12" version="1">MQQQCECKIEGNMNIQIIGICLNEECSEEWFCQNCIGNHDNHKNDLKIGDQMDQLLEKYNSANNQKFIEADEKFQKLQEYYEQIEQENKNETKMLEDLQLFIKQENYDGISKYFEQFKKFKQQIDNNKPASIFKELNRQLNELKQLDFSDLLDDEIAKKNKAKIKEHLTQAKLLFDAQKFNEALQILESSLEINNECVDALLLKSQTLIQLEMFDDAINYCNQIIKLNKQNFEVYVLKSIALLFIQKFNDAVDELKNVHLIQSFRFYYTILNLFQELEGFSLVVKTLTQSPTFNLKKQLQTLITNQQIPSQMVRQQLTIIIEIIKLANQNEIQKALFSFNQNFQVIGDYQQLQEVDLRPNIDPKQWQEIVEYLDQKCKKQDNHNNTQQSPDQFQAQFNNFINDTPSKSAIVQQQAQIQIQNNQITLETQKDVINFLNDTVQPQDTSNSQQQSQLANKPHAKLQQQLADFYNDPHQNERNKQQQQVEQLTDSGKGSTEIQEGSSNIFNYQSYSQVGGISMSVKLDEVNRLKEVLKYIDQLIISNQNVSQYYYKKAMILIQLNQLQQALQCIDLAIRLDNNEPNYYYQKALLLISLGQLQQALSQFDNAIQRNPNCAYYYYEKGKALSRLNRQDEAEEQFQIAKSKEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VLD9</accession>
    <name>A0A8S1VLD9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0700122</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000070">
        <property type="protein sequence ID" value="CAD8178818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VLD9"/>
    <dbReference type="OrthoDB" id="295859at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035768066" evidence="1">
        <location>
            <begin position="19"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8178818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="342" mass="38078" checksum="DF5A366542EFA180" modified="2022-10-12" version="1">MRALLIICLVATIFAVDANKFAVLLQTGTRGNDAVESVYNLLRDLKTENVNVQAAADKKNNTDEEIFTQVIGDLTNVASLNKQQWEALGAVRGDVEAQIRDGYQWLTWAESRLAEIERRNAQLQDQRCWANGLFVRSLADHADAIGVVQLLEQDVAGFLTNNAGVELVQKAETIADKLSAYSHLFQQDALQKFQSLAEVKREGTTGEQVLQILQDLQAELESTLATLQEQEIHAAFALAKYVSDTNAEVAWLNSEHERRTGLVEKLETQLPAVLAQQAKALKLWKDSLNAVAGATADLEEKREFYASETARRQEENAIIDVVIQLFKDQVRSLASQTSLKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VMU9</accession>
    <name>A0A8S1VMU9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0700090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000070">
        <property type="protein sequence ID" value="CAD8178754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VMU9"/>
    <dbReference type="OrthoDB" id="308871at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="97"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="177"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8178754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="354" mass="43079" checksum="2018088AE7388FA7" modified="2022-10-12" version="1">MILPIKYDDYKEFDVNRQLHDQARDFRLVQQELLKQQLYDQQLEMEKRKRSLTTQKQREELLYLENLRKEKEMRNIMEGQNKQFIKKDLSKWYENSLTNQRIIQEQLRQERQQIEQQIVDTAKQGQTMAEFYEKQKKDYYRKVQDQQYGQVQIKKLNEKNYKKFDQAIYQEQVHQENTKIQKKLDNYKQYYQNVQNRQQQLQAMYLENYRDPKQGLDDIILKNVEQKQQQDEKQYRYQKELEDVSKEKYNSILSIQLQEQQQKRKQNRHDAKSYGYQQANSVYWYNSDRQNNISTPAKSVASDQNQLYNPLTNPNPNQIQNPYILKQLGMNLNDLPTPAYTKSKLASMGKFCIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VTD0</accession>
    <name>A0A8S1VTD0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0730129</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000073">
        <property type="protein sequence ID" value="CAD8180051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VTD0"/>
    <dbReference type="OrthoDB" id="1093at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054179">
        <property type="entry name" value="PSD13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035298">
        <property type="entry name" value="PSMD13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10539">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10539:SF0">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22037">
        <property type="entry name" value="PSD13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0647">Proteasome</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="2">
        <location>
            <begin position="143"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8180051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="348" mass="41353" checksum="46BBCD6C4D4150C4" modified="2022-10-12" version="1">MRVYHESKLWKQLSDQLLIYIKDPQVNQGTHLKDLFEGFVKKFYQEIDEMKLVRFLIKAAEQYGDFQSRIDFLKQFKLDEQPQLVIDILIAFFKLQLGKLQEVDELLKQYKQASEKIQEVDPLVYSMLYYLAYNFYKIKNVYEEFYVNALQYLAYTNDQDMLQEQKVQLSYEMALAVLISPNIYNFSELLQQPVLVSLKESAQYNWVYQLLDIFNRGSVRELNSFQWNEERKGVIPNFQILNEKIRIMAFLELAFSLPKNNRVCTFEELAQVSELPLSDIERLVMRTISKGLVKGRINQVKQTITISYVVPRVLTMDKIEIINKKFGNWEKSLNVFLKEVEDVRKTFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VWZ2</accession>
    <name>A0A8S1VWZ2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0780199</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VWZ2"/>
    <dbReference type="OrthoDB" id="288224at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000134">
        <property type="term" value="P:negative regulation of G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000486">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002720">
        <property type="entry name" value="RB_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002719">
        <property type="entry name" value="RB_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028309">
        <property type="entry name" value="RB_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13742:SF17">
        <property type="entry name" value="RE32990P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13742">
        <property type="entry name" value="RETINOBLASTOMA-ASSOCIATED PROTEIN RB -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01858">
        <property type="entry name" value="RB_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01857">
        <property type="entry name" value="RB_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retinoblastoma-associated protein A-box" evidence="3">
        <location>
            <begin position="392"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="Retinoblastoma-associated protein B-box" evidence="2">
        <location>
            <begin position="549"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="690"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="710"/>
            <end position="744"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8182418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="744" mass="88400" checksum="8B4A99F6DCFF1628" modified="2022-10-12" version="1">MYIEEFSNQLNLDQETLDKAKLAMQQFSEQDEKIYVPAVVYLCSQSCQIQQIDGQTKQGNGITLTQIIRNLDPQVDIEQFLMVLQDLPALLKCHSTEFDNVVRQANFSFRLYKKFDKCFSLIQPVQNPSQTPQFKKEDDCDPYILDLKKVAWILCIIVRQNILQYEEQLDKNQLIICDPNTKVILILVGSLSIVFQNLPNTFTQNVEDISQKMLSFFCNDQREIDEELKYYQDLCFKCINQVVNMMQYKTKVLDMQQETQETQLSVLFEEKTIDQTYRRLHKYYEKTRKHSDLDEECFLRQRIVFTPKKDINKQADYSEFETLQKKTKIKSDPPFMQTPLKDNKFSNTQEKTYFDIKKNAIPQTPISKCMELHQWIKKITMQTYKCFEDDDEQEFYNQATINIRKYFEVTESSKSSKQNEQTILTLCLAVIDSLMKSNEGQFDLKLLKNYRFQFAILGLSLYTYQIVIQKPNLLSNMKQLCTIFEISPLDLFRVIANFAHFHRQMPSTITSQLFELEKHILFNLMWQTDAEKLVEIFKDNSPKYFDQELVRRFQNHLALRVKELCKILQLDIHDFAYSAICEFMKNPKLEVLKDVIFDTVIICTFYVIQKHQKQSPSFNSYNKLYTQYYSQQKQVFTRIVEYYNERFLSGFRDIVKSISNAQPIQIPKNFECQSPLIIKQIPVSPNYRQQYIATPEPPSPQKNFMRSLMQKIKPQQQQQQQQQQQQQQAQQKQQKQQDIQPFQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VXE4</accession>
    <name>A0A8S1VXE4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0740175</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the recoverin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000074">
        <property type="protein sequence ID" value="CAD8180542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VXE4"/>
    <dbReference type="OrthoDB" id="288404at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00065">
        <property type="entry name" value="FYVE_like_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000085">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028846">
        <property type="entry name" value="Recoverin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055">
        <property type="entry name" value="CALCIUM BINDING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055:SF178">
        <property type="entry name" value="NEUROCALCIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0519">Myristate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="10">
        <location>
            <begin position="7"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Rab-GAP TBC" evidence="9">
        <location>
            <begin position="81"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="450"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8180542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="578" mass="68577" checksum="1522C93787B87675" modified="2022-10-12" version="1">MIFFQRSKCCVCGKDLQALKLRKSYRCKMCHQDVCINCSDNRVKLYAKPNDFVKDFNLLQRVCDNCYRDYSYYQKQIQEYGLKWNTRSLLQSKWIGKQERKIKMQISISESDKEIIDKDVITGRSEAFLFNYSLREFITQCQEGYDQSYIRESIVKVLQLFVTHYPIIGYCQGMNYIATILLCVSDEEGAFHIMNHIFKSIIPPRFYSNSQGATLIGYQAELYFLKTLLKSLNLQNFDQLSNFLDVSGPQMLLTLMLQVVNTSSLFIIWGEMFKKNSFIPIDQAIILTLVQASKTFDLTKQGIIEEIGKNIKYSDLSQLFNKESAYFTQFERQVQIEQYYSQTSRSWVNNEKLILFRLKKITNFDTEEILQIQNEFKKYCMESRSVSINRQERQSIKQSAQLTDSSDDDSDYLQSLQIQQVKLQKYGINKEAFLDLMEQFHQHYTKYQILDRHKYELVFNLFDENKTELLDFREFLICLSILLRGSFEQKLKMFFTAHTGSSLRDQEFQTLLSIIIPQELQQQQEYQTFLNRIYKHQYTYQDMLQVSQDPFVSENEYKRERSQTSIFIAQSLNHIKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W230</accession>
    <name>A0A8S1W230_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0800028</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000080">
        <property type="protein sequence ID" value="CAD8183003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W230"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46545">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46545:SF1">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="7">
        <location>
            <begin position="286"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="33"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="870"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="452"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="591"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="44"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="86"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="873"/>
            <end position="882"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8183003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="942" mass="113138" checksum="23E41CB737FA02A8" modified="2022-10-12" version="1">MSKSTMQDTKISYDAPPLDFSNLNIKSLADLKHAIPKQGKRKQAPIDEQKEDKKKEKQQQQQQENENIQVQQNQQQEQEDEDKLEGDEKEREKRELQRQKEEKLTPVIVRTIGQPVQQSTNVYKPTIDEKKQTKKQFQVQLIQCSLILSYNKLTSLHNFYNIIDQIMINCKQLQWIDLSHNLIESLDYNFAELPNVKALYLHANKLKDIMEFDKLQCLTELRTLTVHGNPFDAIPNFRLYIIGLLPTLKKLDSVLISKKELDNANNDQTTYNQNMKLNLEILNYDNPLSNFLVKIKYGTQVHQTTVGNNWNQKFTLEVKKTVDLKVEVWQKDQEFHLIGESIVPKLEQIHQLDIVGQGKKEGVLVVEYKEIEPQNPKINQKLEKPQSLIKRLNESLNTDVQQNYGNIQEYFTQQFENHELNHQSKQTASNLLMESFEKTIKGLQINENQYHFDDYEQKNDIEQNKIKALQKELIKKDLKKYEKSRREVNLQILDLDDMDIRFEQQEQKQQILNLRKELVQKLLYIEEKIEHLKRHLKLDQLNKHAQQSIMNSMAEISQISMYREPIVKPEVDPQKEQQRQEFIKKQMKEWNELQEQRLKQIEIQKQKIQKTEEKIRKQQTLIQEAKTKEKREEIEQRLNKLKEKQKQREQDLQEQNKRYLEKKKQHNLAEKYAQQDASYMIQLQEMRKNKLKEMKNPEFNIQEIMQFDKKIKELRKEKAAVRQKEWDEREKQWNKFQAQYYSQSYKRARQYYKEQDLKVDESKIKAQERLNLLRSYDNEVKQRYQPHVSQEKRSEIEDNIRKLKYEPNFKKYRELSEKVKEKHIYFGVEHKDQSGNKKVKLKPLENIPTPISPKRVGDFYLREHAIYNKKHIPPLPRPPEPPKSSQSEQKLVIKDYLKEQRIKKQLIEQQSMKNMNQTLGLENDDQKMIMQIKKQLEKLEHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W3P4</accession>
    <name>A0A8S1W3P4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0820114</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000082">
        <property type="protein sequence ID" value="CAD8184050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W3P4"/>
    <dbReference type="OrthoDB" id="308145at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="120"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="220"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="340"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="403"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="120"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8184050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="485" mass="58059" checksum="24247AFC82400B14" modified="2022-10-12" version="1">MKPILNLDFSEVKFDNEQIPNYTKEFMDQMRDHMYHVRKFEEMQKNQIPKFLPQRPMSAQTKKVKTIPQKKHKYLKQKQQVISNQQAPPQNTSNMIKIAQTYISETIPVIHSNQLIPGSDVQLTKQPNSSKVCKTQAPKQQQQQQQQNNNKPQQQDQPVKKEIYSLFEWEEDIDGQFYIKEAVQLQEKNKRLSTVEKSNREQFLQKVKVQNTTSSKEDLLKKQQFEIQQKQNKLEQLRLNNNVYQEIVNNKKDQEDDYDNDFEDEDQIIKQPIQQEDDDYGKDGFEVYDEDKVVGIIEEDKNQQKQIDILIKRQQNKANTDSKSTLTQNSKNKKKFMYRPKNAKERKQELEGMREELEKNLIQNDSESANLFKLLQDSKEKEKVMIEVNTKRKEDLALARLTQQQLQEQIDQQLHIIDDLDKKEHYAQQIIEKLKDNRKKQQQQYDYEIEKFLACKVIARLLKGKKDRRLFLELRRQMFMNMLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W959</accession>
    <name>A0A8S1W959_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0840085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000084">
        <property type="protein sequence ID" value="CAD8184945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W959"/>
    <dbReference type="OrthoDB" id="286453at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8184945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="120" mass="14850" checksum="21BA33E38DA25425" modified="2022-10-12" version="1">MEYYTEIQNELKQKYNQHYNLYQKQQLEHKVLCYKNNNEDPLKYQQCIEDLNTRMNMNSTTLRNRFNQIEIDDKDCQGKCYDDSKCIQRCQEQSRKKAIQLQEQFYKLMLQENPEYKKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WB16</accession>
    <name>A0A8S1WB16_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0850189</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000085">
        <property type="protein sequence ID" value="CAD8185625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="436897at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02186">
        <property type="entry name" value="alpha_tubulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000005">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000004">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="10">
        <location>
            <begin position="49"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="11">
        <location>
            <begin position="248"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="10">
        <location>
            <begin position="466"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="11">
        <location>
            <begin position="665"/>
            <end position="810"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8185625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="866" mass="95509" checksum="7DFCBB92461CFBB1" modified="2022-10-12" version="1">MREVISIHVGQGGIQVGNACWELFCLEHGIQPDGQMPSDKTIGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFHSVGGGTGSGLGSLLLERLSVDYGKKSKLGFTIYPSPQVSTAVVEPYNSILSTHSLLEHTDVCVMLDNEAIYDICRRNLDIERPTYTNLNRLIAQVISSLTASLRFDGALNVDITEFQTNLVPYPRIHFMLCSYAPIISAEKAYHEQLSVAEITNSAFEPANMMAKCDPRHGKYMACSMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVMRAVCMISNSTAIAEVFSRLDHKFDLMYAKRAFVHXGEFSSHSTLKMREVISIHVGQGGIQVGNACWELFCLEHGIQPDGQMPSDKTIGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFHSVGGGTGSGLGSLLLERLSVDYGKKSKLGFTIYPSPQVSTAVVEPYNSILSTHSLLEHTDVCVMLDNEAIYDICRRNLDIERPTYTNLNRLIAQVISSLTASLRFDGALNVDITEFQTNLVPYPRIHFMLCSYAPIISAEKAYHEQLSVAEITNSAFEPANMMAKCDPRHGKYMACSMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVMRAVCMISNSTAIAEVFSRLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGIETAEGEGEEGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WEI3</accession>
    <name>A0A8S1WEI3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Iron hydrogenase large subunit C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0880133</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NARF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8187072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WEI3"/>
    <dbReference type="OrthoDB" id="10253113at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.20:FF:000042">
        <property type="entry name" value="Cytosolic Fe-S cluster assembly factor NAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050340">
        <property type="entry name" value="Cytosolic_Fe-S_CAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004108">
        <property type="entry name" value="Fe_hydrogenase_lsu_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11615">
        <property type="entry name" value="NITRATE, FORMATE, IRON DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02906">
        <property type="entry name" value="Fe_hyd_lg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Iron hydrogenase large subunit C-terminal" evidence="6">
        <location>
            <begin position="105"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8187072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="417" mass="48132" checksum="3E6539288D845EDE" modified="2022-10-12" version="1">MGEHKLIKSLTMFSSTIKISSVSDYIIPSQECIKPLMKNVKLQLDDPSIQLKPNLIKNQNNVAKVSLQDCLACSGCVTTAETILIQTQSLEEFLQAIKKYQNPAIGISPQARASLSYVLNFTDSEMHSILHQIFQEMNVKLYDMTEYMKIAINNSIQEFKQTNITPLLCSECPGWVCYAEKTLDESIINHMSKVKSPQQIFGAIQKKNHDYIATIMPCYDKKLEAVRQENNEDINIVLSTREIEQFVKDYIQKINIIPQQIQLQTMAINFQEYHNTSSNNYLDYIIQSVLMPNWTVKYNIRKNKDFNEITIYNEKMELQGIYARVFGLKNIANLISQIKTKTCKYKYVEIMACPLGCLNGGGQIQIQKNTEKAIDLIPKLKDIMISQKVQLDNSYQNEQEIFLTQFKHIKNENNFQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WEW9</accession>
    <name>A0A8S1WEW9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0830086</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000083">
        <property type="protein sequence ID" value="CAD8184486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WEW9"/>
    <dbReference type="OrthoDB" id="305595at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="85"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="282"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="365"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8184486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="527" mass="64172" checksum="D711F1EB4ED58212" modified="2022-10-12" version="1">MFFTQTKFHKLSSQKDLLIKIQEFEINTNFDSPRTQKALLKMNKTKYDFRRKNKFEKHNELEYRNKRNNKSLDQQSLDFEYETYLEQLLNDYIELKEQRKQIKEWFLNKQQEEDELRGYEHIPFNSVQSTNTFPYQTITIEDKIGKINQERQRQLSLNNAEVKDELLFELDRRQWEQASLERRQKNAQIRSAKIMEIKIKAQEQNKRAKNKCEKQKKIKEEQIEVDLAKTKQIQDKLNQKDNEISTKKSTIIMEKIEKNKKLEEKILFRKTLLKHQQSETQIQNIRKETQTDENRIKSVERQAKDRQNKQLRYEQQLIRIHEKSALSKAIWESELCNNYGQKMISIEEQKKKNEDLIKKEASKMFKDRKDKLKKHKQNLELVQEEEKQWQLNLQEKLQNKDEISMKLKINKLEEMKQLREQNAQNQRQQLQKLELRKRNQFSQQNLLLERSLNIQKKLEQQKNDQEKVLFYLLTSKKKINSEINNKQQKLKFIHQQSSNQLLEQLKELVPETEFIELQNLKLQIHKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WMU3</accession>
    <name>A0A8S1WMU3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0980052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000098">
        <property type="protein sequence ID" value="CAD8191043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WMU3"/>
    <dbReference type="OrthoDB" id="296325at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="334"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8191043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="984" mass="115995" checksum="99E21BF5A43F2F48" modified="2022-10-12" version="1">MNLIEFMNDFVNNKSQIDISIMKQYINFRNIQQINLEEFGIILTQMISFLSEEGDIQDLIYMSIYMAKHSPNVILITQALNTLTVLLFNSNLIDLSKKLAKFNLKHYLNEQPQLFLISHLNLARIHYCQFNYNKCFRLVERALIKYEPYIFKEMKTNTSKLQKNPSDIILLLNGYLFYGKCVQQLQRQQEMLDIDYDKFFHNGQKICRKFLNSNHYLVTYFTQQRSVSISRKSIMIRQKGKSIKVADAKRISASCNILMRQIKLQTQNNIQEQQQQQQIKAQSESARIDMGESGTKSSERMIKQFRVHSPRQQPIDIQNIIQTKFDHFVQTFSNKKSEPKINSLEKRISELQKENLKMQQQRLEREQEINELKQKIEELGNESNKIKLKYEEQQNIMHDRLSFGNVIPQQFEHHQQNLQFLPPPKQINRQSTTEIRSLIQTLSQGQVEDTVKVLKIPDINLSSSFNDDMEIIIIDQLDQGREYTILLQNDIIVIKKVMFEDILYEIQCSIHGNHLKIQLDVDKIETFDINALNNFFQVAELRAVLPYTCPLKCINSFNKFVQHLILPFVSFYDNQIKLSHTPIGIFNEQNKFWIFEEECQITVVTIEQFILRLIITGQQQQGWIDLQFDQVSLDLFFDSYFYEEYEKESIQQIIRVINQIQQQQLQHTSKKVTNQVHSYVIKNKIRFTQFLESLKQNLENELANIFQIDDLVGFFNFLNGQQIQMPNSTLANLKMCCIKITEQATNQRIIIVADNNQDNCFEILLQNCYDTYAPRGAKVKALGQDQFDYYDVNKTFGLHISLMDYADIQLLCYLLTLMFDLSTFTKELDIDDYDRYKENCIIKKEVNSESCYRFFINSEYRIPITISYLGINDKPIGVKLQVFDEINLSENGMFFTITTGVWDKQKKETIFGQQKKRKPPKYIQCNKMTTDYHLNFILQSIGWQVIFDSVSINQENLQACRILNDNKTTLVDRLENILNIIIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WTM2</accession>
    <name>A0A8S1WTM2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1000171</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000100">
        <property type="protein sequence ID" value="CAD8192091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WTM2"/>
    <dbReference type="OrthoDB" id="301670at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8192091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="204" mass="23627" checksum="7CF98973C1839B5B" modified="2022-10-12" version="1">MIQFMTNHQIIIKSMLVSPSRRLFKKDTKSMKPSMFLNEFNISSLSQLMTVKKEVKMNSTRNIENFGSVTQVNEILENEGILLLPFVEEKQKIRFIKPTSNPKQSLTSRKVIITQSDSNSPLTKSDIYLPIINSIPTSPTKFQIPNHKRHLTNIDEQQKKVEFRKSIQVIDFVNNIITKDVIDGSVKPLTKKLQRQQTKMIRKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WTW5</accession>
    <name>A0A8S1WTW5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T1030184</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDO010000103">
        <property type="protein sequence ID" value="CAD8193228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WTW5"/>
    <dbReference type="OrthoDB" id="313341at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043540">
        <property type="entry name" value="RING1/RING2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46076:SF3">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RING1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46076">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RING1 / RING 2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="55"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="133"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8193228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="276" mass="32641" checksum="CAABA45C6088DD53" modified="2022-10-12" version="1">MLGPLKENSDQSDSEDIIDEQTNNLIGIFKVRKLIDSDKQPTDPQIVKIKDYLKCPICLSLFKQPVYIKDCSHRYCKECIEKNIRLQKEKSCPTCRKRIATRRDLRVDEIVTKILNTVIPDIEQYLIQEDLQIQQEIKNLSQQKQKNEEIQKLVSQKDTELSCNIQLYAQNQLIHQIDKNYIRAPLMTTVADIIQLIGLKLNLPGEFTQYIDIYINNEIQTQMDKTLAELNSEYWGRKEDYQIYNPNDFHKWSSQEMKVATRQIHYSIDRFYQGYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WV51</accession>
    <name>A0A8S1WV51_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ABC transporter family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T1030166</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000103">
        <property type="protein sequence ID" value="CAD8193192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WV51"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18605">
        <property type="entry name" value="ABC_6TM_MRP7_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF330">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C MEMBER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="89"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="125"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="198"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="220"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="334"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="635"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="677"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="765"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="870"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="91"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="363"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="642"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="954"/>
            <end position="1163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8193192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1163" mass="134852" checksum="2B3B350C3B24A539" modified="2022-10-12" version="1">MKVHHEILNEELLQYKSKNYQSKQNLKSIFFTQINKIIQSKERIITIEEIENTLSNSKYLPNELISEYDGNFENNEPILQTLKKMFLKYFIWIGLLKFVSVIFLFTTPVLINYLTTALDQSINDFLIYSGAVLTSTLLSAITDTQYNYQIKILEFRCKSFLIQTLYNSIEAQSNLDLMNLINIDVNTIMNFFISFHEFWSLILKLIIGMTILYLQIQEAVFVGFATAIILVLVNFAIANKIGHYYSSGLLYKDLRMKAIKDFISNPKSIKFLKWENKWESKIMSIREQEFAIIAGSKWLDSLCVLFWSITNTLISSVTLYVYSQNNDLENVFTIIYVFSLLTNPLNSLPWTLAGMLQAKTSFLRINSFVITRKSNNQFKEGIEINQAELKWDFDSEFTLRIPHFKIDNIVFVGGKVGSGKSSFLLSLLNQMKIQSGYFQVNQQFSYVSQQSWLFKGTIRDNILMQSKYNDELYKQCLVQSNLIDDIQKYPEKDLFDVGPDGSKLSGGQKQRLCICRALYNQNDIYLMDDIFSSLDPQVGDSIFNNLLKITKPIIFALNDNYLQRYKKYADQIIIIKNGQVIYDKQEIEMYFQDLQEQTFDLKEQKVNLIQQETIQHERENQQDVEIKKTQKSLSFYLKSLGLLNLLWILVSLALMQISFNVYDIWLNTYINENPMFIFNNSFVFTLFMLVLIDIIIKLSRAVSFAYGNLEQAKKIFVQLLNHVIHAKCSFFDAEESGEILIRFSDDQNVVDNRFPFELNLLCNNIFNFIGTFIVLVVLQYYLILPVIVSALVFYRLQQLYRPTSLSLRRLDAETNSNLATSIFEQFKGILTINAFNKREEFRNIFLSKLIQSTQVQWCSRVIALWLQIRLQLVAGFIVLFILLFTALSLFYELPISKSTLQLLLYYSLTKISLFRDLTLSLTTCEQELVSYERQNKYCNNNIEQDKCLNYSEDIKFKDIKLQYFNGQLALKGINIEIEQGKRVAFCGRTGSGKSSILAVLFQLYEQTDGDMSVPSQQLFEWRNKVGVIPQLGFVFDGTLSENLDCSDQDLISKYVAMDGNSYIDSNSISQGMRQLISFSRVVLQNPDIICLDEATASVDQQVEDFIQQYLRECGKTIITISHRLEALKDYDRIYYLENGLVVEQGTFQELVQLGGGFANLRNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WVN6</accession>
    <name>A0A8S1WVN6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alpha-tubulin N-acetyltransferase</fullName>
            <shortName evidence="3">Alpha-TAT</shortName>
            <shortName evidence="3">TAT</shortName>
            <ecNumber evidence="3">2.3.1.108</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Acetyltransferase mec-17 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1010031</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>L-lysyl-[alpha-tubulin] + acetyl-CoA = N(6)-acetyl-L-lysyl-[alpha-tubulin] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15277"/>
            <dbReference type="Rhea" id="RHEA-COMP:11278"/>
            <dbReference type="Rhea" id="RHEA-COMP:11279"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.108"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase ATAT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.108" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDO010000101">
        <property type="protein sequence ID" value="CAD8192219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WVN6"/>
    <dbReference type="OrthoDB" id="447510at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019799">
        <property type="term" value="F:tubulin N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070507">
        <property type="term" value="P:regulation of microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000216">
        <property type="entry name" value="Alpha-tubulin N-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03130">
        <property type="entry name" value="mec17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038746">
        <property type="entry name" value="Atat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007965">
        <property type="entry name" value="GNAT_ATAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12327">
        <property type="entry name" value="ALPHA-TUBULIN N-ACETYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12327:SF0">
        <property type="entry name" value="ALPHA-TUBULIN N-ACETYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05301">
        <property type="entry name" value="Acetyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51730">
        <property type="entry name" value="GNAT_ATAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="5">
        <location>
            <begin position="1"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="189"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="105"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="141"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="site" description="Crucial for catalytic activity" evidence="3">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8192219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="266" mass="31328" checksum="325BCDCCBA2EE33C" modified="2022-10-12" version="1">MQFNFYLSKCMSVNENGITILNGNQKRNPQLDVVITIMGEASALAQNLKQVITSPIRFFQTDQKMYIKSEGKQCLGILKIGRKNLFHRDLNGVIKEIQPICVLDFYVHESVQRKGIGKELFEEMLRQEVLTPEKLAYDRPSPKLLGFLKKYYNLQNYIPQNNNYVIYSQYFDNKQTNIFQQQLTQQTHQQQQNPQLVNQQRVQQQQTPQKMDQLDHMMQQMSLESKQQYSQYQKSKGQPPWATDQKQYQNMYQTTTGLMSAQIHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WX35</accession>
    <name>A0A8S1WX35_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1060181</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WX35"/>
    <dbReference type="OrthoDB" id="308434at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="4">
        <location>
            <begin position="152"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="245"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="486"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="97"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="491"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="503"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8194413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="541" mass="63917" checksum="A7247163E8CF3435" modified="2022-10-12" version="1">MQQNNSAIKHLIKPFSFELPQSEQFNPLLLDNLNWDYVQANLDIAQCGPFLKNFVQSNFTSDTIRNMEFRQFKKMLEVCQFAMDFYTQNQEQKEIEIDEKVQILRNKQEQLEKLKEIEKQHMDKQNQINKELELLKQEAKKVKEQAVKNIIFKCHHCDKCYPSKQELLKHIDNKHVEKQKDDITNPEYLGSMLQQLQQQQTQQQQSMANTQQSFMKQQQFGNTMQSMPDPLMIGSAGFQQLKQQQIEKMEQKQKRKEERKIKRENDREERQKRILQQEEQMKQFQNQMKILIEQKVQEMVKLNNMVGSQQQAQIIQQKKLIAELEELKQMRFVQKDHSIIQQEQQQVNQSKSNEDEKILNIIDKEVNDLDISQQSLHSQQLSRDQKLQINKNQKAGDQSGMMSFHNYSYSQNSQNMINVKTEMGKANQIKNNQLNVAEMAHVGEIESDNEVLDQDGDASYLRPVNKKSEVQQAEIEFIEAIRKKKILKEDPQDDDDEDDHDFDDLLNKEKQKLLGPNSESQTNKELLESLNKIQQLSKDKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WXT9</accession>
    <name>A0A8S1WXT9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1040085</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000104">
        <property type="protein sequence ID" value="CAD8193437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WXT9"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019185">
        <property type="term" value="C:snRNA-activating protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001006">
        <property type="term" value="F:RNA polymerase III type 3 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042796">
        <property type="term" value="P:snRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051575">
        <property type="entry name" value="Myb-like_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46621">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46621:SF1">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="115"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="175"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="175"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="322"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8193437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="480" mass="57129" checksum="4886CE669B8A59CF" modified="2022-10-12" version="1">MSKFSQFLEDISDFKERPVKLKQIKKVLKINEKSFPPCKNILNYYFQEFSKRTNEEHRTKWHRKDKILFVWTVLKYFEFTNKSDLNPSEDDWHYLSNVLGVTEQLLNLKWISMLKTNLKMAPWNPEEDEILQNLITDPNENKNWTQITIDFNRINPAKIVRHAKQIRERWNNYLNPELKKSEWSQPEQLQLLLLVQEIGKRWSLISKKISGRTENQVKNQYNSMMNTYRRQNQLDNEDIAIKKLLANLQGKDYEGPLPLTSKRIRKTVNNNNNNNNNNNNNNNNNNNNVTLYQNTINDQKNIQIESNEIKEEHQQDQQLQSQQILQQQQQQQQQQQQQQQQQLLLSQQQQIQQQQQQQSLMLGLPVFSPMLPFSPLHYLIKSSPNNISSPYEMRNKNYSSQLQLFDEYMKSTNNLDQSAPQSEVPCLLRLFNSPAFNHQGFPYATMPYQQPTQQQPAYNTKKINKIQKELLKFDKGLNRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X306</accession>
    <name>A0A8S1X306_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1080028</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000108">
        <property type="protein sequence ID" value="CAD8194909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X306"/>
    <dbReference type="OrthoDB" id="325070at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="203"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8194909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="270" mass="31037" checksum="1B49175022124035" modified="2022-10-12" version="1">MEPLRNSQVCPTPLVKDQQELLNTIKPSSLNQVNQPAGSENYQSIDIESNQNCSDLLKDQSFIFKKYVGLKALLLYFIQHFIAQMLILSNIFFDKSNIEQGVSTFLIFTYFGIIIMTRFQSLQKVPKSYIIFVIILILEAQIFSQQLEVMNQQISEISPIQIDKDNLILNDVGYQCFILFLETSTLIIYLLRQKEDLKLKNFFIIYGIISIVPLLLSSQFAYISIIIFYRGFALSLTIQQQFKGRFSLKINSIFGMANSLNLGFLIPCNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X621</accession>
    <name>A0A8S1X621_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1130108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000113">
        <property type="protein sequence ID" value="CAD8196717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X621"/>
    <dbReference type="OrthoDB" id="290751at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8196717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="636" mass="76239" checksum="97A253823ACD1364" modified="2022-10-12" version="1">MKIFSLNFSDQEMEHEYQKEITSIYKNYQKTQKWIVLPLVVFQISIKIMREDWLLLTVLLLCSLLYALNLKCASSSNRYFKDLLLFMSLVLYNFFFPYNSVFQKKELQLQTYSLDGYFQALTILALMNTLSYSKKQLILVVIFTFQITLFRNPNIQLWEQVVLHIFELCIYIHYCYCFENFRRKIFIQANTQKILEDNIQTYNDIESFIISYNPPKNKLILMQKNRQQQITEQIQDEFIDKIRNTKVSSYDSKRRFTQFTSQEVLENQANMTLENFLFYEFTNKKKYIMHLIHQSEQNNSYLLKGFLYQESYTIKVVKLYQDKPCALVLLMENKKDNVIDQLNLRNKIAMKALDQIGDVFREYVKPSLISFQWIYNNKQKNKNIVFINKLLRKVNSNLLRCYNDFSNIQDYFSINKEFQRCLIQKFDIKEFIEQIYQHVVRFFGDDKGIFFTIDCKIPDTYVRQDQRQLKQLFLNLFFFILEKQSGSKNNHHNYVHITLVQEKDESNQQMSIKIRIDYEGHHLSKAAINSLPIINPQTIGELKHNSQKAFDLLLPISLLIIRKIGPHDKLTLKQNRMNRNFMEFQIFNALEENYHLLPIISFYPKDNILTTSSLAKHYSEYFDQVPIVQLNSIQEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X9T8</accession>
    <name>A0A8S1X9T8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribonuclease</fullName>
            <ecNumber evidence="2">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1160106</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RNase HII family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000116">
        <property type="protein sequence ID" value="CAD8197774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X9T8"/>
    <dbReference type="OrthoDB" id="7462577at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032299">
        <property type="term" value="C:ribonuclease H2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001352">
        <property type="entry name" value="RNase_HII/HIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024567">
        <property type="entry name" value="RNase_HII/HIII_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954:SF7">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01351">
        <property type="entry name" value="RNase_HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51975">
        <property type="entry name" value="RNASE_H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-2" evidence="3">
        <location>
            <begin position="24"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8197774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="281" mass="32199" checksum="52B6B6BC952863F0" modified="2022-10-12" version="1">MNVNNNISIDELPQNLEDLKTNSEWILGIFEAGRGPLIGPIIIAGCFWPINNHNYIVKQCSLSLNKQFYQNQTESSEIISKLKGQALQFETIEISAQKLSTKILAYTPSDLNEISLNYTIQLIKNILSKGYKIIECYINPIGFKENLNELLKKGLGFENNDLKFIVGIHQTIIAAQIVAMQERNARLLQIQQDIYPSGQMGSGYPSDNQTREFLSKVSIPFFIYPEEIRFAWQTISASFKQKAISIEYMSEENWTKQTKEQFIENPSSLEYGGLHFENYYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XA81</accession>
    <name>A0A8S1XA81_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GPI ethanolamine phosphate transferase 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1170042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000117">
        <property type="protein sequence ID" value="CAD8197997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XA81"/>
    <dbReference type="OrthoDB" id="299042at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051377">
        <property type="term" value="F:mannose-ethanolamine phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039524">
        <property type="entry name" value="PIGO/GPI13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23071:SF1">
        <property type="entry name" value="GPI ETHANOLAMINE PHOSPHATE TRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23071">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="411"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="440"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="462"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="493"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="525"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="550"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="636"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="668"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="707"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8197997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="738" mass="86230" checksum="A1BFB04C1C7677CA" modified="2022-10-12" version="1">MQNLINMYSQFILSVIGCFLIIYSTFWFRTPIKDINDIVAFRKPMYDQIVLFFLDATGYRYIINENTDHQIFQTITILNDLNHNYPLHTFLQKTKAVPPSTDFTILSIINGIKQMQNNIDVIFKSNPAPYEDSILRKQFKSALYSSKKHDWLRMTSGEFTYVENPQFFSLENIQIADLSSYQFFKQSFQQDQPDYQLYVVHMMGFDALGHALQHQDYDQATQLFSMFNSILEGVVNNLKENQLLIVIGDHDQSKRGKHYQCSQESSECQGFIFAFSIDGLFQENQIYDFYEPTDISATITSLLGYKSTSQNLGKIIPQFYPRKTNISEIQDDQERIKKQVIKYLQTQGYKISSTFIEELKQLSADQVVFQCQRNLQLGLPRKESYFIGIILLLLCIYKMQDHLGLKETIIIVSTGFFNAQIMIIGLLVLVFLRREYKFKLILKILVLFIFIQLYEMGYIPDILLSIEIQFLLILAIKYQNFILSRLKFTKWKISLLFSLVFISQSVVCVFFFLDQNEFELRKSLLQSLISVIILFFVIDPRYFNKGRVKALYLTILFILSDVSVIFQLNYSMNFIQFLLQFMLLQFKDVGQNVLISKILYNAINPIKINHQVFKGYQQINMNSMILLTKEFDIGPSLFSVFIGSFGCQIIGIASLFFNQLNQDRHKIVGALIISSISNLMLNSWQILNYNQSIHNTNQMIEEQITLINNLPLKLLELILYSFAFSILVRQIKKQREIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XBT5</accession>
    <name>A0A8S1XBT5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1180045</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000118">
        <property type="protein sequence ID" value="CAD8198272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XBT5"/>
    <dbReference type="OrthoDB" id="298965at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035833566" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8198272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="155" mass="18892" checksum="97B0055224291DA3" modified="2022-10-12" version="1">MKLFLLVGFILSFGLTQLIQSELGINWEPKRDAVIKKMKEIFTYYNKYQNFDVALEKQRLAHTYILLLTEKPDEKVDAHQLQTLIYYFEIQFIEIHNQYIDNQIPNDKYQPVQWYFDYQYYLKFIYWPIAFIVGLFLVNLIYYCIADNCLKIKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XCH9</accession>
    <name>A0A8S1XCH9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Casein kinase I</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1190164</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000119">
        <property type="protein sequence ID" value="CAD8198764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XCH9"/>
    <dbReference type="OrthoDB" id="291494at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001186">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="14"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8198764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="455" mass="52471" checksum="0DE6DA555A43F918" modified="2022-10-12" version="1">MLQIQIGDIIDNQYEIIKKLSQGSFGIVFYGKSIKTKKSVAIKVEKQEMAGFGSLFREVDMLSKLKGVPQIPELLWYGEFKKCNIMITKMLGHDLIYHQKQYKQFSSQCINSIAYQLLWILEQIHKQNIIHRDIKPENILSKNESDKIYLIDFGISKNTDSNFIHKKKLLPFIGTSRYASISAHQGIEQTPKDDLESLGYVLIYLTTQHLPWMNIEKADVQRLDKIGKLKENVLLEDLCFGCPNSMLKYMRYVKDLKPNSKPKYGILRGLFLSKLQNTIELGLDWTNQIIKIEKKKHHKKQRNSCDLNFKSCVTIRKPFMGETQINLNVKTIAVQDEQLISSDNGTNSQIQFSIIETLGSKNSVKVGFSASDNLDCLCIQQNNNMARISTFEGIAEVNSLKSQSFEQQLMESEHQDLEIKCNVLHFNSVYFNFKNPIQQHIIKQNYQVKVIKSIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XD91</accession>
    <name>A0A8S1XD91_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1210020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000121">
        <property type="protein sequence ID" value="CAD8199137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XD91"/>
    <dbReference type="OrthoDB" id="429991at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8199137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="252" mass="27615" checksum="29FB445D2FD49D12" modified="2022-10-12" version="1">MNQYLATHDSSLNTSLSKQKYSFPRAIREPIHNKSATNELSYKLPQNLGSRMAGIGYGHKHDFSKDAKPVPAPNLYEIQSFIENNIDKKKGPTFAYSRDQMKAHGIWGSLNQLSPGPGRYASTKSLIDSKFTFGLKPGSLKQLNTPGPGSYEAIQITNQRGNQFISKFRSSGAAIIGKDESRFKTLQTMNSFPEPASYDISNTGITGTGFCPKNGLKSTVQNSFPKAIRKGPFDLGAQSPGPGSYKAYSEFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XDK7</accession>
    <name>A0A8S1XDK7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Calcium-dependent protein kinase 1</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="ORF">PPENT_87.1.T1200087</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cell membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Parasitophorous vacuole membrane</location>
            <topology evidence="24">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="30">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDO010000120">
        <property type="protein sequence ID" value="CAD8198953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XDK7"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020002">
        <property type="term" value="C:host cell plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020005">
        <property type="term" value="C:symbiont-containing vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000398">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000299">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000383">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000828">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 26 27" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17" id="KW-0969">Cilium</keyword>
    <keyword evidence="15" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1032">Host cell membrane</keyword>
    <keyword evidence="16" id="KW-1043">Host membrane</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0519">Myristate</keyword>
    <keyword evidence="12 26 27" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0564">Palmitate</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 27" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="28">
        <location>
            <begin position="26"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="329"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="366"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="400"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="29">
        <location>
            <begin position="436"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00060437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00068067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="EMBL" id="CAD8198953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="470" mass="53580" checksum="A92164BFD4C209C0" modified="2022-10-12" version="1">MDNNIVIKKQWFIKSSATKIEDAYEFDHKKLLGQGTYGQVVKAKLKGSKYYRAIKIIPKSKVRNPDRFRREIEIMRNLDHPNIIKLFETFEDVRNVYLVMELCEGGELFDRIIDKGHFSENEARITILQIMQAVNYCHQNGICHRDLKPENFLLLTKADDSPLKVIDFGLSVIFHDNHVEKLTQGKVSMTTRAGTPYYISPEILDGKYDESCDIWSAGVILYILLSGVPPFYGNTDPEILDAVKKGIFTFNIPEFKKVSEGAKDLISKMICKPEKRLKSHEVLQHPWMKQQQPGGSFLSVNYQSLKNFTNFNKLKKVTLTYIASQLSEQEISELGKLFKQLDKNGDGVLTMDELTHGLTGLKKESQNEIMSVIKSIDTDGSGTINYTEFLAATIEKSVYMKQERLFQAFKMFDLDGSGKISRDELKQVLGKNGSGFDDNTFKALIADADKDGDGEIDYNEFIEMMDKMKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XIG8</accession>
    <name>A0A8S1XIG8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1240052</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000124">
        <property type="protein sequence ID" value="CAD8200302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XIG8"/>
    <dbReference type="OrthoDB" id="286314at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8200302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="125" mass="15274" checksum="03A0979AEE2A389E" modified="2022-10-12" version="1">MQQQQQQQQQPRARTKERYVCEAMNLVKLWRQVYQTETRVVDGRTVRITLDQAAELVGCPRKTLEDYYYLLKKAQYLVNLEEKKNEKMGFIRKICRENKKQQQLLKQEEEFYQINQFQLDEIHDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XJ12</accession>
    <name>A0A8S1XJ12_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Carboxypeptidase</fullName>
            <ecNumber evidence="6">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1260174</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="6"/>
    <dbReference type="EMBL" id="CAJJDO010000126">
        <property type="protein sequence ID" value="CAD8201153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XJ12"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000408">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF113">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE CTSA-4.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036516320" description="Carboxypeptidase" evidence="6">
        <location>
            <begin position="21"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8201153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="439" mass="50765" checksum="2557EF1198C21C09" modified="2022-10-12" version="1">MKILLGLIITSLVLVKGTDQTSFFAELLGEDLPYYTETDYVKFKDVGSIYYHLILKEGTPNLEAIQKGDVLAIWLNGGPGSSSQLGNYMEIGPWVIKKNPDTEAKEKPYIVTKREYSWNKVMHLLFIDQPFGAGMSKADKENVVTNSDQAANYFVETIKQIYTRLNGLDQVTTYIFGESYAGHYIPAFATRLLKDEETLKKVNFKGIAIIDGITDTQNQLNYYHSYLYSIGAISEFDLERLQKIGVKGQQYIMNGEYAKGAEQLDLMTEDKFIEQIGNINVYNIRKYKGLDEYDYSWVEFLNTYIKEFSSEITQFQKSNSKIYNAFQQDIGESRLKDIELLLQKKYKVLLLNGQLDYIINTPGAWNWIYQIDWTYKYQWKNAQKKLITFPIQGQENKVETHGYIKAYENLQYATIYKAGHMIPTDNPKAAYYVIENFIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XM69</accession>
    <name>A0A8S1XM69_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">1-phosphatidylinositol-3-phosphate 5-kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T1290110</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000129">
        <property type="protein sequence ID" value="CAD8201944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="312434at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000285">
        <property type="term" value="F:1-phosphatidylinositol-3-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00065">
        <property type="entry name" value="FYVE_like_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17300">
        <property type="entry name" value="PIPKc_PIKfyve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044769">
        <property type="entry name" value="PIKfyve_PIPKc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45748">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 3-PHOSPHATE 5-KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45748:SF7">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 3-PHOSPHATE 5-KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="9">
        <location>
            <begin position="18"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="PIPK" evidence="10">
        <location>
            <begin position="1130"/>
            <end position="1451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="899"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="779"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="910"/>
            <end position="919"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8201944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1454" mass="171307" checksum="B7526844A7F6178F" modified="2022-10-12" version="1">MDQQLSGVLIKRDKWKAESKVTKCEHCDRQFYYLFRTKHHCRKCGLVFCSDCSSNFIDGIHFVENTEKKVRLCGVCYDQVLKLLKAQGYKLENTIETRVVSVSIDKGQLNLSRRESIHKLDDQSYTISRSQTTQVTEEQEDNMNNDQEFNPEIVQVSIQSYFPPEEQNKNKESELLKSQLSLLKDKCYQYMENICDHSLKIFIQPQRNHDNHFDGLKSTTFEFVKKAVEDIQFSSLNSDPLDITCYVKTKLLPYKDYSLTCYFPGIVIRKNIALKRMQTELFKPRILIIHGNLDFIEETQQFDDFILKEKRVLQDYIEKIKENFKPTIIIVEKSVSKVALDICCKFNITVVQNVKIHQLRKIAKSTGSKFVRLDKLDGYIQKETQVTGNCEKIFFRNFPRPNPDKQDVAGKDNTLMFIETKDGKNGVTIMLSGPQEDLLQKLKQCIIGCMRLGKHFDVERYIILCEQKLRQNKQFNFCVGDFTKFLFEKVNLKEVIKPDLAYVKINYARADIHNFNDIKDMNALLAYQKEFPPQKEKVVDFFADMCNMPTEKPKAYYHSNDDMSMGAFIILKVANLQFRCEYCKLPRNSHVSIYYNAGRYVKFSVDGQMNQIKQIVLQKEQSQIQQSDENKTFLLKDLPSIIKQNHSNEKIQIETYFECNLCMQQLTDRVILSQKYLEYSFLRFLQQLFLTQNITINNYHNISSCNHTQIQRVYSYYGYQMKVMVGEFDVYQTTLLNFQDNSFNDYLKVWEQNYIKQLRDELMNKLNTFGITLINLIPKSLAKSEIDLLIKSIKQLKEECEKIHAETQYISIFQILKQSQDFGLQYKNIMQSIQEHKKKKELNPDFKNIIQNAAVNLKNQESILTNNTNTIKTTYELEKVDSGNNLNEVKQYHSNQELEDHHLHSGQKHSMQEIEETNHRASSGKTDLSDLIIPPLYSPELDIQQQSPLFVRNHRRNGSEIHNNNNYVINLVDANQGLLQQSIQKTHHDSNKDLDNVNINQSNNFIPTIDINQNNNFQKEWNISSFLAEQQKLIKSQMIYEFVPIYENQPLSYLSFTLNHPKYINEIYQKENFGMIESVQKSSEQAKLFFQQLLSPKEQKQDVENIRYMSVDLGNNNINQQQQIYYKQDNRDIFVLKINYDVEKKNEKSNNQIFQQQQQFQNNLASQFDEAQTIQEKSLPLNNPQQFVPTAQARKNKWIEVLIYFPTQFEALRLLAGITLAQFIKSISSTNIWSASGGKSQSKFYKSNDELFVFKKLEQDKEFMMFKQFALDYFKHMYRHFYESKPSLLSKIFGMFEIRDRGQTEYYLVMENLYFGMGDPSNLLVYDLKGSETNRLEKKKKGVLLDTNFRIDRNSEPIPILKENYRYNDRAFQIDCKFLNKQNVIDYSLLLIIDQKQKKLRMGIIDYLRFYTWDKETEHYLKYLLKGGMVPTIVNPGDYKKRFINAILKYFIPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XMN9</accession>
    <name>A0A8S1XMN9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T1310036</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="EMBL" id="CAJJDO010000131">
        <property type="protein sequence ID" value="CAD8202435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XMN9"/>
    <dbReference type="OrthoDB" id="272303at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF203">
        <property type="entry name" value="PALMITOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="28"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="99"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="261"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="261"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="286"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="296"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="322"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8202435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="457" mass="53089" checksum="30E1465897BF3F07" modified="2022-10-12" version="1">MDVDVKQGRLRPVRRNGLINGMNPYQCLSWIYTLFDIVIAYIFAFQFEDTGMKELQLILLTVIVTCIIYSCLRAALIDPTDSIVKEEQLSKLHGKEFKTDIKSYCLVCQAHVQDKTKHCWSCNKCISKFDHHCIWLNNCVGDQNYSYFFVLVLSLVALKIFRLGQDFKLLYLQTNFDILVYICIAVDPPVLIVLIYLLSMHLYFKWNNISTYEYIKSKKEAKDKLMKTQNVQNKQIQNESTTGYGQLLSTSKRFDLKSQLSLKTGDTKKSNPNYFSNKQEDEKKNNQQTQQQLTQIPSLFTSKPTTPGNDQDKKQKISQLSKPKDLDDLKDKQIYNQVIVEDSEPQLNESSDEDDHFNNEKSKNHCPQNSHISELPNNIEQIIVKDDQINEKDMIIKQEIEIKPDKESENEDIVSNQQSLHAQEPPLPSKCYTFQLEQQVDPNNMMSNNNQKLYCTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XMU6</accession>
    <name>A0A8S1XMU6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tubby C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1310163</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000131">
        <property type="protein sequence ID" value="CAD8202666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XMU6"/>
    <dbReference type="OrthoDB" id="290698at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000007">
        <property type="entry name" value="Tubby_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01167">
        <property type="entry name" value="Tub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubby C-terminal" evidence="1">
        <location>
            <begin position="156"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8202666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="241" mass="28729" checksum="C2228E5D012B191D" modified="2022-10-12" version="1">MLPLPVQTRKMPKLDLMDLIEQGFGQNMSLYSEIPSELQYCQFHKYHQKNDECMIFKEDYSIYMYAKKYKNYWQIYTRDPSDSQFDKENFEWILKIEDDVLSLWKKPNQFISVVKIIHCSNYTESYNRMRLLISNGKLNNVPLQFDSDYYPNGCDYISTIPPIYNSKKRVWHMQDSERMQKASKRNFLLGLHCNEEKKQVLKMGKIQTGVFIVDFKEPINFVIAFSVALATCDLKSRIRDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XQA2</accession>
    <name>A0A8S1XQA2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1330154</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000133">
        <property type="protein sequence ID" value="CAD8203349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XQA2"/>
    <dbReference type="OrthoDB" id="291124at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8203349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="206" mass="24324" checksum="8EC387A7F26F4D21" modified="2022-10-12" version="1">MLYKPLYYCDPKFLDRNQQQQQQTKPHLLPEIQMNQQFKSLQNIQHTSRATDALLSGRNSLFDQLQEPLTQRSSYQSIIKNKKFSLNQEQQNQKKVLLVQFEDMGIQNNQPKSILKRKNSNSSQQSDVLDFFELVEDQPVRNKKHIILEPLQSTPSPQMANAISPKRVSFNKQIQVKLIIDDYTECNEKRIQSRHHLRRQTTDFIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XZ72</accession>
    <name>A0A8S1XZ72_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1460027</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000146">
        <property type="protein sequence ID" value="CAD8207051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XZ72"/>
    <dbReference type="OrthoDB" id="301307at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8207051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="295" mass="34832" checksum="B88DEC19FC4E457C" modified="2022-10-12" version="1">MGEELILKIVDVIPPSSMQDTDNIIKATGEWYTKDKEAKIIIDFGNALISLISIQNYYADQVNITLKGDNSDDIYYDKVIWKQNLDWNKSLSIDIEPLSQDIFNQMIVEVINYTQKDYKGLKRIRVYGIQDKQLYNERKKLAKQFKKESKDKQKVQKALQNQEKSEQKDQKQKTKLDESSTKKNQSWGLYNKSQNQTNKGLIFKDTREKLRNIHKSPSPIKEEIEQFNQTAIQTPKQLEGFEKLGSNINEWKMVRYNMNQIDKQFLELHQELQKSSGIVQDRQLNDFNFEVHWLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y1U9</accession>
    <name>A0A8S1Y1U9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1460115</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000146">
        <property type="protein sequence ID" value="CAD8207227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y1U9"/>
    <dbReference type="OrthoDB" id="1926212at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="170"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="306"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="340"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8207227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="392" mass="46097" checksum="8CD687BBC3F98401" modified="2022-10-12" version="1">MISSNEILIYCSNLDHKNCVQQVCFNLFCKIGRLQCVQCIKNEEHSSHPEDQYEIEDMVKYIQEIQTKCDELVDKLSSYVYLIEVELNILKQGLLNKYQIPINKLNKLKNIQINVAFDEMLKFQESTSLITQNLEKFTNEFINELKRNYDELRLKELNYLELDKKELQEAEQLYKQGYNLRSSNKYEEALNFLDQSIALNPNHLLALSCKAYCLRMLSKFNEAIIWADKALALDPKHLNTIYVKARSLYCQLKYTQAIKWADIGLQIDPNHQNFYDTKVRSLILIGQNHEAITMINKILSINPEDANFLKLKGQSLMNLKQYDEAISFCDKALKYENNDEMIYYIKGECLQNQQKYQQALICYDQALQIYSEDITIQNKKAECQKLLKMQDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y1Y0</accession>
    <name>A0A8S1Y1Y0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1470048</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000147">
        <property type="protein sequence ID" value="CAD8207375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y1Y0"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="142"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="94"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="94"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="114"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8207375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="487" mass="57104" checksum="A64E3DC13ED0E459" modified="2022-10-12" version="1">MLYNSIFKTKNQSPNNVKTKQQKRQSSIEKPKKERIKLQLSSKIDQFEKEAILSMINCKKKSVNKRLKTEQGIDIIDDYLILDQYNTNKQYKSRSFSNAFPSNKSADRKDNILNNSSKRQQNNKQKYSLEDDLCLDNFPQQYHKIKLLGKGGFSLVWLGEHKTTKMRVAIKQIKQSNSNQTYLREIWFGSHFFNNGIPKPQFKSILGIKSLVQYLGYDIGRSDTWIFTEVCGENLKNTLYDLKGQQIKNEIVFKMIQKPLYICLKQDLNILKKIIKDLAHALILLTEQRIVHCDLKTENILIKKSRCLNGSYIITQTKLIDYGSSFIFDDLSQFSMATPEYMSPEILNYIQYENGKDYDIKLFEVLQNYYKPWVIDIWSLGCVILEIVYGIPLWLQNKIIVKQHNKEVIEQGLFAVKNRSFDQIIQKQAYVIKNLDYFLDNNYSGIKVDLEIRLLLREMLTINPDKRIAPHTIIEFLQPNKGRLKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y2Y4</accession>
    <name>A0A8S1Y2Y4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1500106</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000150">
        <property type="protein sequence ID" value="CAD8208366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y2Y4"/>
    <dbReference type="OrthoDB" id="303271at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8208366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="209" mass="24929" checksum="407DEB8B1EC35F50" modified="2022-10-12" version="1">MNQTQVFPLKKRNSHPEQEIRNTHLKALEYQTTHKLQRKQSKSQVQLECYSENEENLDDYQSQIEYNFNTMTSLNYQQVPSLISILTSESNLSIEEEFEIWDDKSDLRVLKLILDEQKPSIQQFLLLYKARFGGFPGQQQCIKNEEVIKTAVTYASSNKQLFKKYFPSKKIVFEDDIDYSIPYKIEDDHHFQSFNHFFKFQKTKTLVEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y378</accession>
    <name>A0A8S1Y378_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1430067</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y378"/>
    <dbReference type="OrthoDB" id="188293at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035885143" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8206174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="152" mass="17869" checksum="948C7452ECBF3D5F" modified="2022-10-12" version="1">MLLFILFPIVFSQTQVCQTSDFNFGYKKVNIVICLFFQTTDNQKYRQLTNILHIQYLQHIKLFLQNKVVYGLISDIISHVYGTPYLQPGGAFPIQSIVIGSFIDVFIFWNNVCFDGSFVRRTKLLQLKDQIFKKIMILLFHVMEVLVILKYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y4C7</accession>
    <name>A0A8S1Y4C7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1500007</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000150">
        <property type="protein sequence ID" value="CAD8208187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y4C7"/>
    <dbReference type="OrthoDB" id="306078at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="420"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="331"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8208187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="578" mass="67845" checksum="A8AD1620C64A7D31" modified="2022-10-12" version="1">MSIPLFQISPLPKSISVQSNNFKNCINVGSSLDCALFIGIIEYHIKVNKVEEIKRIYEKAQAQSVGKIKTIISNSENQQQRLEYILKEFKQTTEFCKNLDQMMGNYFKNKGISNEKDKDKDLKNKVKILEQDFNIRFYLYDSKSNLKIQDIAATTIYIYRSDSNFYILINAQVNLIACSKCNGQTNLQKLKCNHMACFQCIQSQFEGKNISIVSFPCFVKNCQQKIEKSYYLQMKLPKSLDQKQYIKQGDNSSKQSLNQVEKKQESYDNCVEEKNQINCMNTQHILTSSKSEETQFDKNMNQEKGKLQIQVSSKLEKQQKGKFEQTEQPIQHEILNTLENIKKNCQNQKQEEIKAQKQLHNKQCSHCLKECKEEIFEASCGHCYCPKCTQILTSKNKSYTCLHPKCNNIKKIDYKLETKCDQCQDIRDRTLLFTNFCDHQICVQCLEGLVKNKRNYKCPICFSNLQSKDIELFFENLQFHIYEQETQKNSLKQDQFDFQQQILQEPNYPKYCTFCFSPFTEFNLQQQLSHCKNPLHMIGVCCSIFPLDCPQCQIPSLEISKYQIIFEIRQYFEQNTLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8B1</accession>
    <name>A0A8S1Y8B1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1560033</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000156">
        <property type="protein sequence ID" value="CAD8209780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y8B1"/>
    <dbReference type="OrthoDB" id="320047at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8209780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="146" mass="16889" checksum="FAAE55E7EB89C4BF" modified="2022-10-12" version="1">MNNLVFSYLFSKGLQILIQQLNTPDIIAVIRLQAATRAQNATILTMKRVFFNNKIVKYYLVPQFILFSIPSIKTDTVLFTLSFLLRYEKTEDISKKLHINVIIANKEIIVLKVVSKFKLTICNIDVKVNSQKLDVQILINPEQIKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YA92</accession>
    <name>A0A8S1YA92_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1580005</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000158">
        <property type="protein sequence ID" value="CAD8210108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YA92"/>
    <dbReference type="OrthoDB" id="19588at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13431">
        <property type="entry name" value="TPR_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="166"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="200"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="234"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="302"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="336"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="370"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="438"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="472"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="506"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="574"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="608"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8210108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="676" mass="79813" checksum="B035CE6735DB168B" modified="2022-10-12" version="1">MSDDDLKRKEVSIQMKNQNLKAFMNDVSLLIRYNYLQRLIKHQNEQNIAIREGCFYKERLFQVQNIIVQLNLIKWLSKNILAKNGLKSKTIMIQSAKGKKISKIVYKEELIEAKKYFKNENNKKDRNQNSNGFLIVNNLNKVNNFEETLKLYNSVLEKDLENPENPEFFHSKGNTLVRMNRLEEALEYYDLAILKNSENSDYFNNKADTLDKLNRFDEALQFYDQAILQNPNKSDLYNNKANTLGKMNRFEEALEHYNLAIQKNQENSDYYYNKAKTLSQMNRFEEALQFYDLAINKNSLNSEYYNGKAILLKTLNKFSEALNNYDQAISANPENSDYFFNKAMILSRMNKFEEALIYYDQAILISPQNSIIYHNKGNALTEINRFEEALQCYDLAFMNNPENSASLLNKAITLDKMNRLEEALKIYESAIQNDLKNSDSYNHKAIALTKLNRFEEALQFYDSAIEKNPENPNFYNNKAVTLDKMNRYEDALYYYDLAIEKNPENPEFFNNKANTLDKMNRFQEALLCYDSAIEKNPKNPGYYDNKANTLDKIERYEQALLFYDLAIEKNPENPEFYNNKANTLDKMNRFEEALQCYDSAIEKNPENSSYFYNRAITLDKMNQFEEALNYYDLAIQISPLNQDYYYGKAVTLQNMNRWDEEIEFLRKVNFKAQFQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YBC5</accession>
    <name>A0A8S1YBC5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1650034</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000165">
        <property type="protein sequence ID" value="CAD8211776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YBC5"/>
    <dbReference type="OrthoDB" id="286322at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8211776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="107" mass="12584" checksum="E6002F61F86109F4" modified="2022-10-12" version="1">MTKNAQQQFINRTYVKVSAMQKCQLIALVFQEEWKIKKAASFLYIKYASAKSIILKHRKNQILTKLPMIPEDRRCHYKILKNGKHNYNILCLKGGIFQNILENLSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YHG1</accession>
    <name>A0A8S1YHG1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1800016</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000180">
        <property type="protein sequence ID" value="CAD8213505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YHG1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8213505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="394" mass="46229" checksum="4129D3D21C1EA40B" modified="2022-10-12" version="1">MVDNSAIRSIQFQLITDIFLMRFTPQKQYQLTQKYIRIKQNNSNIFNKQSMINLFINKNNREYRTQIKIHQKQQSSNLLIDQDNYVNNLTYEVLSQLIYAADERLNYFLSNQQESQQQPSKQEEQKGEINEVQTLAHIKTQNSTVHIYTLDIKEKPLIRTFAPSLEFIGDNKAPPIINTQRIIITSNQVNPFQLKQKQYQLITLLNNSSSLEYFLFQPTTLQIIKENDPAIICLQETRQIINTSPRYNFINKLHSEHFQGGGISIGIHKQFICRDISHLFKLDEIEYLLVQSTKIMQNYILNIHSANSNSFRNQQEKLQIFIVQLKTKKPNALKMIVRDINNSAVKLTGMIDLIPKYQITYQRYAKYKLLYSKTDIFQNNQELSSSISFAKQSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YHN9</accession>
    <name>A0A8S1YHN9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1760019</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000176">
        <property type="protein sequence ID" value="CAD8213269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YHN9"/>
    <dbReference type="OrthoDB" id="292058at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15332:SF175">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13948">
        <property type="entry name" value="DUF4215"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="829"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="924"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1033"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1112"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1177"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="230"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="301"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="335"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="372"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="417"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="456"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="565"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="600"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8213269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1236" mass="142589" checksum="83A054A09A8F6E75" modified="2022-10-12" version="1">MDQINVNFVKHHAQSVKIMVIIVLIASLLLHQVRIYNTNVNAHMVIFSLILKHVKNAQVHVKLVNRVKITVYLVLIVIKQQIIHFFVNVIQVGFQILMVSHASNANYHVQIVQIQQINVLLVMIQYINKLSLVNVSLDGYLMIITFAYLVKSHVKLVRYLLLNARHVQILIMNLITNHNVLITRAKCQEPCYECDINGYINCIDQNQILDSNKQCICKSGYLQQEVWCLQCQNPCSTCINTVNECVTCQDQNQIIKDYKCLCKEGFVNKGDFCCDQYCIDCQGIDNCNNCIEGFYLSNINRCLKCIDHCDICYNQINCEICQDGYFINESRLCEECIPSCNICGNSFSCDVCLDGYYLVNQICEQCNQNCLTCNELSEKCLTCKSNYYINSNNKCICQVGYYEQQNSCYRCEYPCKTCLSQTICQECVLLTNLQLDLNFQCNCNSGYFWNKNNCSQCYQSCLTCIDNQYNCLSCNQKLHRILVNNKCLCDQNYFEGEDGVCISCLDQLGKSQESCKYQDCKDQIWTYGEECDDGNDVNRDGCSNCKIDPNYSCSNEILNQSICFQCSTNCIKCQLNPLTKKSKCIKCQIGYFLDKNDCVECSINCLECLDQAYNCISCKFPQQKNNKCQLCESGYYADEINGTCLNKCGDQIKVNEEQCDDGNLIKDDGCDDQCKLENKHLHHIIPNYPKPLFQSVGTQQIYSATRLFKLSYTTPIFITDDFQIKDYLSFHIQNKENVQQMDQSINLTQDNSLINEFNQSQLILMINITFSRSSQDEILLIKFLNNSVIYSNEGYFQIETEVSCSIPKVTYIDDVTIAQVQIATNSNTYILYFIGAMCGGSVLLGGVEVFFNLLDTLQMLSYFKYINTQLPYNLQTYFQLFGFAQFNFIQKIFDLSGFIDQILKTQQLKKIPTKVAGDNLTSLFIINAATITTVWISLFGIYAIAKLIPKILYSFKFKFFSESETENSWLVKVGVYYLTIKYIINRLCFTIISEFFYSGILRAHMATAYDFTFSIVLQLYALELNSTNFFIRFSSLLAFFASAIYIISIYYVIQVSQMKKYALNNKAIQAKYGCIFDGIKINQFSKYLNTILLIKKLTFMFLLIFAYEFPTFQTVSITLLSTSMSLFYIFFNPLKDKLEYFKQLFSEVSISFTLLSITILTCDFELVYFSYQVRQYFGWGCIFFMTLVLCIQLAIDGFQQWKFLFKKYKQIRGLAQSILGVFQKNRQVTAQSYIFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YI23</accession>
    <name>A0A8S1YI23_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T2090005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000209">
        <property type="protein sequence ID" value="CAD8214366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YI23"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8214366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="309" mass="37244" checksum="AC6181D3704F9311" modified="2022-10-12" version="1">MIEKEMSLNCNQKHKQRIEKVSSAPQLNKSDKLLCYTSLLNPKFDDKQINFNAIFQVIQVKYKFKKQQFENIILIQDKQNNFKEILVFFQSVKIVQFTNEWIQILNNLFIKLLNIVYLIQFIIQLEMTRQYLKINKIYLNQLTQLMIIQQKEFLIKSFENLKLNLVNINQEQDKQILLLKNKSRMRKVIQYKQIIFINNKTYYHLMELLNQYIFKLQTSSKNRVGQLRLIKIEVFLYHLIIQIILLFSILRMESKTIQEHLTFQKYQMQSIIQPLQVQMVEEFICGYKLMIGSGEDHNFIRNMSIGQII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YMT5</accession>
    <name>A0A8S1YMT5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T2490005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000249">
        <property type="protein sequence ID" value="CAD8214781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YMT5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035712460" evidence="1">
        <location>
            <begin position="26"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8214781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="61" mass="7186" checksum="72FFD19EF666CA6C" modified="2022-10-12" version="1">MMKNMCKFTQRRLLCLVLLVTGVDVTNNDFQVWLKDKEKNSLCVVGRLFELLLKSCNFKIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RV61</accession>
    <name>A0A8S1RV61_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0010330</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the inner dynein arm light chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RV61"/>
    <dbReference type="OrthoDB" id="273640at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019347">
        <property type="entry name" value="Axonemal_dynein_light_chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13183:SF0">
        <property type="entry name" value="AXONEMAL DYNEIN LIGHT INTERMEDIATE POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13183">
        <property type="entry name" value="AXONEMAL INNER ARM DYNEIN LIGHT CHAIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10211">
        <property type="entry name" value="Ax_dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0243">Dynein</keyword>
    <keyword evidence="3" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8131876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="236" mass="27532" checksum="2299B5E4B07324EC" modified="2022-10-12" version="1">MNDILPAPSLVKYSNPILISNSQKKQIKQSPPSNTEDILNAILLPREFNHQKNQLQIQCVSPAPSTKQDVLELQDKLDKWLQQKLARETGLCPIREELYSQCFDELIRQITINCAERGMLLVTVRNEVRMVIQTYQTLYASSIAFGMRKFLSEEEKKAEQRFRIKQLEQECSELQRQVENLEQKLQDTKHQDQQNREQTKETHMEIINQLKTDIKNKVSGDLDKILTGQKKLPDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RX81</accession>
    <name>A0A8S1RX81_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0010676</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RX81"/>
    <dbReference type="OrthoDB" id="286792at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555:SF170">
        <property type="entry name" value="FI18122P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="2">
        <location>
            <begin position="11"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="305"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8132566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="390" mass="46190" checksum="BBF834D43E586BC8" modified="2022-10-12" version="1">MDQQTEETLNLGIDVQVCDPIVKKDGIKNYVVYTLKGQDKDGQFEVIRRFNEFDCFRTTLQIRWPGCYVPPLPIKKPVGNMDQKFIDERMHYLNLFMNKLTTINHLWYSEETKLFIKGGGDIEKQLLALQKPTPGDIIYKYETIFKDFSGKEINDQLLSKIGNFSLFLKRIQPQLETFQQQAKQLVQSRIQCQENMNLLLDYLMPEYEKNCLTEYVVNPENKLVFVQHNTELYQQYRAANEKSALDNFALSIKIETRDIDAVLEAIQCKDKYEQIKQNYLTKLCSLSNEKKDIENGKTTLKSFFNSNKEEFIQKTQQQIDNIQKEIEQLTILCDMITIILGYFIIPKYKQEKEKNYYQLLRQMAQHQVQKAQAERNYWLDLDKNQMFMEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RXB6</accession>
    <name>A0A8S1RXB6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">NADH dehydrogenase subunit 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T0010402</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the complex I 75 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RXB6"/>
    <dbReference type="OrthoDB" id="10249365at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.740:FF:000030">
        <property type="entry name" value="NADH-quinone oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.740:FF:000001">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.210:FF:000002">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 75 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.20:FF:000002">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 75 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000283">
        <property type="entry name" value="NADH_UbQ_OxRdtase_75kDa_su_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054351">
        <property type="entry name" value="NADH_UbQ_OxRdtase_ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010228">
        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019574">
        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015405">
        <property type="entry name" value="NDUFS1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050123">
        <property type="entry name" value="Prok_molybdopt-oxidoreductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01973">
        <property type="entry name" value="NuoG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105:SF13">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 75 KDA SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105">
        <property type="entry name" value="RESPIRATORY NITRATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13510">
        <property type="entry name" value="Fer2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22151">
        <property type="entry name" value="Fer4_NDSU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22117">
        <property type="entry name" value="Fer4_Nqo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10588">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09326">
        <property type="entry name" value="NADH_dhqG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00929">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51839">
        <property type="entry name" value="4FE4S_HC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00641">
        <property type="entry name" value="COMPLEX1_75K_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00642">
        <property type="entry name" value="COMPLEX1_75K_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00643">
        <property type="entry name" value="COMPLEX1_75K_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="11">
        <location>
            <begin position="25"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S His(Cys)3-ligated-type" evidence="13">
        <location>
            <begin position="103"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="12">
        <location>
            <begin position="240"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8132020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="710" mass="78607" checksum="AEE69AA7A55A675A" modified="2022-10-12" version="1">MIRFSLKKAQQLLGKQFQAFSTQQNLIEIFIDDVPHKVDPSATIFQACYSAGVIIPRFCYHERLAVAGNCRMCLVEVEKSPKPVAACAAQVAPGMRILTKSEKTRIARGGVMEFMLANHPLDCPICDQGGECDLQDISEQYGYGDSRHNEYKRAVEDKNFGPLVATSMNRCIHCTRCIRFSDEYAGVTELGTSGRGRNTEIGTYIDKMITSELQGNLADICPVGALTNGPYAFTSRPWELKSYDSVDVFDTIIPLIQIDTRGAEIMRVLPRIHEEVNEEWLNDKSRQAFDGLKKQRLTLPLARDAQGNFTDLYWADAIQQAAKKLQSVKGEEIVGVIGEFADCESIVALKDLLNRFDSDNFEIRGTGVPQLDADFRANYLFNSRITGIEEADVLLLVGTNPKIESPLLNSRILRATRKNNLKVFLVGPANDLTYNYVHLGNNASILDEIANGTHPFAARLKSAKLPMILTGAGVLERVDGNAIHNALKKIAQNSPVINPQQGWNGFNLLHKDVGRINALELGITARRSSVPPKVVILLGVDNNLNAQDIPKDAFVIYIGSHGDEGAYYADLILPGATYTEKNGTYISTEGRVQTTKLVALPPSGAKSDWEILRALSEECGCALPYDSLEEVRYRIAELAPHLLKYDYIEPTVLGQVALKPESSKQTISATPFRDLIDNFYMTDAISRQSVTMAKCSTAFNPHKFSTFKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RXS1</accession>
    <name>A0A8S1RXS1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0020060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8132766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RXS1"/>
    <dbReference type="OrthoDB" id="298993at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035830013" evidence="1">
        <location>
            <begin position="22"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8132766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="359" mass="40091" checksum="5C29CEEE82BB67FF" modified="2022-10-12" version="1">MQKIALLALVLAVTAAKFVDTHTTLAQINSDPFGHVVLSAIKAHLQASTPANEVNMLLNGVGAGLVQDQNDHDHAFELDTTTNNRIVEDLEKEILYHQNQIASNTQLRDDTIEALAVSEEDIRVTISDIANNEATYAREEATRNQQHETFVSKVAAIDDVIDAIDEAAKLIQHLSLGASFAQLKSKYDTLHKKLSDNTSHTALLQPVITALTELATHGVNQKALTKIAQLLSEIRQQLVSEKAAKTDVEDRQAAHWAEFSVHLSNEHTRLVERKAQLEVQIQEQKDTIEDAQSWIEFHTLELENSEERLAGQQAWYAVQSEIYETQTAERTAQQEIVDRLQEHISEKLSTTAQFIASRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S2I3</accession>
    <name>A0A8S1S2I3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0030286</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S2I3"/>
    <dbReference type="OrthoDB" id="300505at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8134396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="144" mass="16995" checksum="6C29FC34A7EE309F" modified="2022-10-12" version="1">MNIFDTKANSEGFFSKYGRLTSQKFYILRKPKVIIKQKVHQEENSLFVEEFPQESPKNKLLIDKNLFPIYQLKKSNLKNLSKTFQFQTPLRSRSLQFAKKQSLQNSETLSFSKTSKKIGESFDSKKKNFNYSRVSGELEQLQYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S2I5</accession>
    <name>A0A8S1S2I5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0020581</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="77931at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035773852" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="16"/>
            <end position="2815"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2426"/>
            <end position="2447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2473"/>
            <end position="2498"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2510"/>
            <end position="2530"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2560"/>
            <end position="2587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2625"/>
            <end position="2651"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2700"/>
            <end position="2718"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8133735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2815" mass="328745" checksum="94142EF6A5FD3050" modified="2022-10-12" version="1">MIFAICLCFLLTAQYQFPSTTLYLNNLSLRKIITINEAQLSEESCLTFGIWTKYNPLNIKAFKDQTGMFESNCFQLINVMEYTTQNLNFIYYVCLDYSQKSIVKTIQFVNDQDEQIKFEKNVDNYQYENIWYQFQLISWPILKRVEFIIIQSEITIFKEVLMNMKPFKEETLLFIFGESLEVQESKIRQIEKGKLFSLFPGSIVVYDPLIDIIPLQFDFEQEIIEGYYGLEQCNCENNEEVNLEDYDFNLLEEKNYISEHISCDFYSLGGWLRVSDIVQKSEEFFYSFIKMTAYIGEQTLLNENLGTFELIYQISPIETKIIVTAYSYDFPIFTKEILDGSQKIETEFQIQRNIITSWHYLLLELKDNELNIKITFYDQYLKNNQFYEAQVQVWQFHNCQYKIRYGNIQQDLQNYVNISVRNLQFINCQYDQRISRCHPNCLECDGPGNMHCLSCSAEDQRVYHAQFKQCICSYSTFEYKNKCVSYQDSNLSLKRRSQNTNLKCKAGYFEFEGDCVKCPSRVKNDLVTCFDCYSNVENWSLQSICQNDLYIPLSLEIATTLILNFPLQFLYDGFDLIICDYCFIYNNNQELQNEDSVYELYLHKSSNFKFFCSQLSEYNEDKRCTLCNVAYCLKCAIQLEGMVCLLCRENLVLVNGECKNQINNYIYQSCQPPYYTSYLKECKICPINKCQYCFEYFDEYRYMNTLEKGEKDFQVIDIDLIKIGCVLCEDGYIFNFNLGLCLKQASRIENCLTSYVLQLEEICIISQLNKFQVSLIINDCDNYIINCNICAQDYDKIVFCIECKIGFLLQNRQCYLSEEQGSSYPQDIRDLQVKAFLLVYNFDSYKAQNYNQTPLSNYCGKNCLKCNKYDEQYVCDECKLEYFEETIKIQYQFYCPLCPRLCQVCKSRSSVQLQAMNPLFLFTQENQKNFIYTQQCLLPIMDPFVFYDPYQKITKYCIDDNCDNEFQLETYEYSCFFNRFPTTLHYFNINSEYYNHIGMQTFTFNVYFEIYQNLCIMVPSFQSRATIKEEVFALNNAIINLISLNSVFFHFGNSILLYDYDTVNFIDFGFVYPIETQENFQLFNSHNQIDLNIINCTIQNSSITNIRSIFQADQFGQINIQNFSIINTTIKNSSFFNFQLFKFEGQMTIVSLYIYNCTIIDSQLFQFSQNLYDINIQNMTSDSNIFKNASIFTLYINYIEETTLFFKDLLIQRNQFDHSYFLFCSQIITIDISYVIFNFNQIENSIIFGFSYGLFAKYIQIKENQFIESQFLQTIEVTNDQNTLYFITELIVEKNVLQNVNLFHIFSNSYSNKLQISISQVQIQLNTKSITTDQTSSIFNINSKTLDINNCIISNNQEIRIFNLYDNEDIILSNLIFENQEMEQKVSVQKNCFQRINLQNQLILVMGFDKIKIEKCKIIKQSSVDESLIEIVSSKFKQSLGQIIIKNIEFYGNLIQQLNQVQSFSMLAILSENQIDFVMEDLKYQNNFMHVYTESTLQQSASLLYINSAPSTIYINNLNGIQNSMTNSSNSFIRITSKDLKLSNILIKNHNILPFNVWKDYYPINIMNVEDQKEINLFIMQIYHIKTIGGAAQFEVSKLECLNCTFQEILAAKSSVFDIVTQNEGILVIQNFQVDTTTNSLVEQTESSGCLSVNSQNSLLSLTISQAKFSNVINRMSTSIFSIIPSLLQNKIIIQDVSILNCISLKDQIVHIKFINQIVALNSILFINIKIKQDQQAWTSYFQIIGLIKLSEILELTGDENALIYLENCDFEIRDLVVEGMLIAPIIKLINVQNLIIFNCNINDIVSMISLDLIQISQIVFIKSLVYIKQLNIKSVKLFKLDTIPIVQPAQFQADYFIDGCSFMNNSFFAESPADFLHTNINQLQKSNYQRNSVISIMSQQNLSTILLEMITIDQNTFETTLNGIIYFDVAYFQKIIISNVICIKNIINEFGCLHFVGNNSVTSKIYIKDSNFIDNKGKKGVAIKITDISFDIKNCKIASNVASTQGGALYLKINSKIFRILKTLIINNHAQEGGGIYFEKDNNLNNKNFNNSFLLFNSAKIYGNNLVENPTHLTLYINSKEMASEKQVQNNTSTCILQIKPYTIIEQGIYLKTKKLMIPSNQEINVYQLFILQASLYLSYIKNMSLFFKNSKGEILFNLNSSTCHVSSFIVTNDGEEVKGSTYNHTLQYASATNNFELGKLAFSLNPYESNYSHLKIQALCKTQYSQQEFKYIINAQTFKCQLGEFYIENGCQICIWNQGFYSVTYNAIKCSIYDKQKFQSITSNMINLYEGYWRPNPLSDYTESCYKNPSFCLGGWEVGDSTCIQGHKGALCEMCDTYNERGDGKFFKDQQNFQCQSCSKNDNIILPLFFALFQALLSITLSLKSINKSNQLFASLKIFYKFSKLIFKLSQDHEGILLKLLLNYLWIFSVIFTFNINFSFSFIFIEQSSNSFYFMVNNLDCYLSDFQIELIYIKIFVILTLMLLQFNLILALSFLYHNITNKNMDNSLLSNTLIYLYVFNYGGLIKMLCSSLSVRQISNINYIQGDVSLLYDTSNHQLWIFFVILPLLLVFGCIIPFSLFLLTYIKRDILHKIKLRRHICYLFNEYNDSNYYWEQIKLLQKAIIILITTYFETNILLKTSLLGLSLLFYQAIAVKNKPYIISKFNNLDLQSGQICSITIFLAAIQYESQNINNQYFSLFLQIIIIILLVRLCYPFILNMFTIYSKKYSLFFVITIHSCLKYLKKNMQCDQVLYNYILREKERKERLKKNFQKLKEFLIPKSKISLQKYKAVTLKKRLRSTTNSGHYLVEVVNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S4W8</accession>
    <name>A0A8S1S4W8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0030230</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S4W8"/>
    <dbReference type="OrthoDB" id="5586at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21504">
        <property type="entry name" value="PPP2R3A_B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.230:FF:000007">
        <property type="entry name" value="Phosphatase regulatory subunit, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.220:FF:000003">
        <property type="entry name" value="Phosphoprotein phosphatase 2A regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000025">
        <property type="entry name" value="serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041534">
        <property type="entry name" value="EF-hand_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14095:SF0">
        <property type="entry name" value="MIP22305P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14095">
        <property type="entry name" value="PHOSPHATASE 2A REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17958">
        <property type="entry name" value="EF-hand_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="325"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8134290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="476" mass="56994" checksum="BA137EAC5502718D" modified="2022-10-12" version="1">MTMSGMSQSALLSSPNKKSQGPSTPPRADSFHEIKQRIPEDLSQSLTQSQIIQKKLYVDEVQNYDLVPQFYFPTQEINLQLQQEQNKIINEIFGKAENIDANHFEQITTNLCGLCKYLTRILMTAVEGTSNKISKTAFIKYWNQQLSQKEPKQRCFHILKKAKNDFIQFDDFKPFMKILLEQHPGLEFLQATPEFQERYAETVIHRIFFHLCRKDNNKITWRDFKSSNLFDILDILGKEDDINKIRQYFSYEHFYVIYCKFWELDGDHDFHISKEDFSRYSSHGLSRKVVDRIFDQVPRRFRSQIDQKMSYEDFIYFILCEEDKTTIQSIEYWFKIIDLDDNGIITGFEMDYFYEELKQRMDYLNHEPILFHDFVCQMVDLLHPENDILFKLSHFKQNLTVCGVFFNFLTNLNKLIAYENRDPFQVRNDIVDHPDFTDWDRFAFQEYVRLAMEEENQEQGEVFEGDIVWDNEDSKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S5G1</accession>
    <name>A0A8S1S5G1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0030516</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S5G1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="96"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8134813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="205" mass="24651" checksum="CB1C7AB22EC7D3BA" modified="2022-10-12" version="1">MNKIYFPKRRKIFLSTNYDVPKIIVKTEENEGISIPNETRFKNWNDVFRDFLSETLLKSKQTQLKRNTETSIDRAYLYEVPLQFDNALSISPSKRRKFLLKNLQLEKINLKNLINQFHQLSRQEKDQKINVTYHRSAVIDEGNHDVAFLQLLDEKNKVMLKKQQALDRFDIIRDLIAEKIEKREKEDLVQKKQRIKKFWNDQINV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S6D7</accession>
    <name>A0A8S1S6D7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0040080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S6D7"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8135153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="283" mass="33658" checksum="AFD7F388D52F8E35" modified="2022-10-12" version="1">MGQEQSNLPSNNLVVQLHMQNYEKLTKLIEQKLSDQTFTIDKRINQMDDTLLHYAAFKRDKKLVLHLIAKIRGIQLLEMQQMIKKQNQCQKVNENLMNHTIIIYIINQIIKQLLIINMSAKKNTNQFEQDSEEEQIQKKIKKNEEQKKSDEQNDEEFEVDSELQEDEEIEDDGIQTSSHSSIDIQAYLQQRDQIPDEDDDDDDNQQKQKKQTQQKQQQQKVSKKQQDDDENDDDEDDDDDIEDEDEDDQEQVDDDDEDDDENDQPPQKKQKVQQQQQPQKKQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S6W6</accession>
    <name>A0A8S1S6W6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0030509</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S6W6"/>
    <dbReference type="OrthoDB" id="295910at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8134799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="881" mass="104989" checksum="28A5898E49584F3E" modified="2022-10-12" version="1">MDQIEFKTLDEFIRLFHKKVSTFTTSEMQIQLQHLAQMLSEKQIQTEAKNDCFFKRWSEIKLPISLITIDFISQLFVIYTKVNETLKEDILLDFIWPLIVYEFEDKKVVVEYFNIMKTIYDKKYRELLKSFGILEIVKYMLKTTEIDDMICCEQHNNKEQPQQIISQEIQTKIDQFGLFINEIIHQMINDLKQTTGNNLNDLESLFGISVHQLLSYCTKDHNCCQVQTLLIILLNQLPQVNTDIIQKVFQNFEAISFIVLLYNSTTSQVLQVYCIKLFQVITNKNPSIIQKLILSIIPSSKNKNFLQYLHSEPLICSILEWLLNIPTTIKITEYQMFDEKLTFKNHDVMELTLNYLYICPVMMKQQLITTLYTLMRNNKENQLFLLSNKHFLSTFPKFLMSLQDDDFLETDENQNSLQKSIYDTSLKLISTVFMNQFQNGKIDMLFQWLKLAELEHIKQKNEDLDGFKDARTPSYFIRQLITYGFNNILKSSILNTSELSNEQLWRGFHLFIASFLAIITKDLIQQDQKISKLIGKKVAYSPQLLMITQLFENQNWIDYQFCIRIIQIVNFLYSQTISMNLDIIKPSINFKTSEFALWLNQLFKKILSNEKDNIFLMRIIQTLICYYQHYIVAQNFGQSQLEEIQQLLKAYRHFMQSLICGSQQFKNLKHIPVDIEYQITLSHYFYIESQMKLINQIKQKCQKDHHGSHDSFFNLYALQELIAYIQQQLQEYYLSLIKLCQIYGKRSQIFIWNFCYELIRIKKYEGDVLESYLQTLINQPDIIHDIVVSDEMIVYYYDCPNMVNIKQKSQLKLNELIKWANLLIQQQEHEQILLYQEQYSRQSQILMKDVTKTLDKYNLIQLQNYEIFEKEKLKYDQSKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S787</accession>
    <name>A0A8S1S787_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0040305</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S787"/>
    <dbReference type="OrthoDB" id="8300214at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861:SF1">
        <property type="entry name" value="TRANS-ACONITATE 2-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861">
        <property type="entry name" value="TRANS-ACONITATE 2-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase" evidence="1">
        <location>
            <begin position="37"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8135595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="289" mass="33168" checksum="0F24A2C16C9CCAC9" modified="2022-10-12" version="1">MNQPKIWDAYSKSYHNIEKNPAGFQFSLLNMLRVNEADEILDAGCAGGHLHQYILQQRKKDCRLTAFDFSLEMTKIAAARMIKYFNNPLNSQIEDISDQEIENVDLNSPLLEQNKYKVFQGDVQSLPQLNDNQFDIYISNLVLQLVANKDQALQEAFRVLKPGGKIGIVIPVVLGNVPMLSLQKCFIQAGLIPNAAYKPTELENREGLINFLYKHGFKEIVCWNQTIPFDLFDIDDNYIFPQFREILDKASKEQVDQFYSLYKSEVQNRLNLNLPFTVEALSLIAKKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SAK4</accession>
    <name>A0A8S1SAK4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0050497</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8137088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SAK4"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8137088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="169" mass="20466" checksum="8AF3856B39523D86" modified="2022-10-12" version="1">MKANRFIIHNPTIESYQLEEYYNHFKRMQQIDKDKINIYKTIEQNKSATNSRFLMLQHTSKFNKQQQQNAQDKLQKLLDDKIERIKVRENPYFNINRFRNNSQTDIKSTNNTLNTSLENINLIKKLQTLKPNISKKNLDVYYEKQQEYGNRLRKLPKLPDLDNKSHQKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SBG4</accession>
    <name>A0A8S1SBG4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0050041</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SBG4"/>
    <dbReference type="OrthoDB" id="310866at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8136224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="269" mass="30936" checksum="8217CE357099725D" modified="2022-10-12" version="1">MQSNNPKLGLISNQSNQKYTDTQQENDVFELQDFGDDSDQIQFDDTICQNKAIVLANLDQEPITMVTSNNRSNQSQQVNQKSQFQASYPIKQQVPQYQMQNSNNQTNQNCFGTFSKEEREQSNSNQISQIQNENVNTTNQTQSTCMFCQIPQMIPKNKNVFICYNCKQINKLIFAYFICGTCRLTVMYQQGISNLINCTNCMSINYVQQPNLPNTFSQVKFPTIQVQYLVNQTQTISTISNQQQGQGIQQQQQRNQVSDLEAEFKELTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SBP8</accession>
    <name>A0A8S1SBP8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0050437</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SBP8"/>
    <dbReference type="OrthoDB" id="416585at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005245">
        <property type="term" value="F:voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044581">
        <property type="entry name" value="TPC1_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46988">
        <property type="entry name" value="TWO PORE CALCIUM CHANNEL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46988:SF2">
        <property type="entry name" value="TWO PORE CALCIUM CHANNEL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="117"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="300"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="390"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="421"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="449"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="507"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="556"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="595"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="11">
        <location>
            <begin position="392"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8136970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="661" mass="77522" checksum="FE8EC49FE92360F5" modified="2022-10-12" version="1">MQTELEAQTPNSVRLSEISMSDSKIDDFHQVGRIESDNDFASYFALTRITKKIQDSFIQSNDPKAQFIKETYSSIKLIRKLFVTIYILMTIFQKPIWCNPTQYSNDCQYELEQGYQIPAFLTFYIPVLAYYLIEIFLIISMIIFKAVKYYSFHTILIERKIVAYCFVMVGIFIISNDIIVIIYDNDYSYNISTLLKPIFLIFYSQYFLGIIMDYALIIYRGKEIYFMMIFSYVFFVGLGTAIFRNQSEISDIAQEEGICNSSGLPFSQKNYCTFLIMFQLQTTVNSPDIYLPYYGNRSVAVIYFIAYQFVNTILMINLILSLFYSQYKKLVEERTKSLLEKHRNKIQQFQNKVFRNKRSNSIVGIQKKQQQQQQDQTNSQFLIQRILDHWIFNSIMQILCIADFCFIFQDNTNRDIQIVNITLNSIMLIESIIIAILRDLKKIHKYPGVIFDFTISLILVILIIAIMALQQDSFTQDILIKVCCSIMSLRLFRGCTWLLKSKNFVNILFKIFSISTYLLQLFGTLIVTMTIFNAIGQLIFGGKINYNQQHINNYQWVNFNDFLSGFCTCWFLLIVNNWNLMSYDFSLSLQSDVIYLFFIFYYIIVVLLAQSVTIALLIEYLVNHVKDLGQSSEDETKSEISIEENYQNELLVPLQQNFKAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SDX3</accession>
    <name>A0A8S1SDX3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cation/H+ exchanger transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0060475</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8138148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SDX3"/>
    <dbReference type="OrthoDB" id="196264at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098719">
        <property type="term" value="P:sodium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110:SF187">
        <property type="entry name" value="SODIUM_HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0915">Sodium</keyword>
    <keyword evidence="8" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="40"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="97"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="247"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="301"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="337"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="374"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="417"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="11">
        <location>
            <begin position="22"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="517"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="523"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8138148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="607" mass="70024" checksum="EA606DF05BF5778A" modified="2022-10-12" version="1">MSGMESFGVVSITVIVVLFVYSISGAFFEHKHIHIIHETGLGILLGILSGFFFYLVLEGTLRSLYTFNGTFFFYILLPIIIFCGGYNLNKRRFAQNFFYIMLFGLAGTILTFAFIFIFTYIVNEQNLITESFNQDNVIKLSVENIMIFSATICASDSVAALTMIKPEKYPKLFSVVFGEGMVNDAVSIILFISVELISNDNVETWKMPLELMWLFIKEFFGSMAIGIIFGLFTSLLFKRLRFLTESVILEIIILLYVGYASFAICELLELSGVISVLVCGITLAHYNYYNLCNLGQISSKITLNSLSLFCESFIYVYLGLALWAIKGENGLDNMKTSWVFILLEIAICFVSRGLSLFILTMISQIFTGRKNFKLTFWELNIVWFAGLIRGSVAYALIQKLHYKEEEKEQVQLMQTTILIIVVITTIILGAMMPFYISGNLQIQEHRNNKKKVQSRDSIRVTFLGEIQGGLGENDDQQSKKKSKFYRWLAHIEEKYMKQWFIYNYSQRRLEIKEQKQLQKKANDGNQPRRQTVQFQRQDSQGYNSRVSKVIHRSTINSKYTGIQQIIHCIEVPNEIQYYHDNEELKTQVQDQNDFREVDENLEQSNIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SH52</accession>
    <name>A0A8S1SH52_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0080262</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SH52"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8139723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="152" mass="18729" checksum="1CA23D5AAE6CF40C" modified="2022-10-12" version="1">MKNYKTSNDSVYKRFVFYDNFVNLLFINFINTYYFILMTNLYKGFFNKSKKYQQQIQMKKINILKNNQSLKVEKKHCFEKNDSKQITIQKHPKRNWMHKKYFLFEKAAKGQYQFKQIKLFIMDLNFILQRNLNASRFLDLQKCFNVQENQSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SI90</accession>
    <name>A0A8S1SI90_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Proteasome endopeptidase complex</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0060457</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8138114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SI90"/>
    <dbReference type="OrthoDB" id="37597at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF3">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0865">Zymogen</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8138114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="250" mass="28216" checksum="45D0712C2247B886" modified="2022-10-12" version="1">MGITEFDLQPLNKIKVPAEYENDVQFYTEAIMKQHEKKRQRLLIEYNELLNLKQVESLQDKKITAQFEKSQDAERTMAGGAADCQYWLAYLANHCRTYELKNGSKLSVAAASKFLQGILIGYRNSGLSMGCMIAGTDTTGQHLYYIDNDGMRLKGDIFSVGSGSTYAYGVLDNHYKYEMSLNEAVELGIRAIYHATHRDTASGGVVRVYHIHQNGWTKVHDGIDVVDLHYQFAQQKGLVGDGDEAKQRLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJ30</accession>
    <name>A0A8S1SJ30_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ABC transporter family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PPENT_87.1.T0070083</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="298706at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001172">
        <property type="entry name" value="Cystic fibrosis transmembrane conductance regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000233">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000255">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003449">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF456">
        <property type="entry name" value="MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN LETHAL(2)03659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="200"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="229"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="306"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="688"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="728"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="821"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1018"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1058"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1151"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="86"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="416"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="751"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="1081"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="1340"/>
            <end position="1574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAD8138374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1582" mass="182195" checksum="97C24E3CE9DAAE5A" modified="2022-10-12" version="1">MNLRDQAQLLDVDNQIEFNQKNCGSMFFFGWIYRLLAIGRKKPLVQSDLTIIDQDCNMGTSYEKFSSIKKQCLWHQLFFSFKGLILFCTILYLLIALLSSCQPFITKHSIQFFSYDQGQKPSVASMLLFVTVQLIYKLILSLLKPYQSFYICLLSIKLQGALSQEILLKTLKFPIQRSKHYSTGELINMMQVDIHQASNYFYYAIILFTVPLQLFIAFIVVFITLKGQAVIPAIGSVLQAVIGILFGIFYGILQKRYMVAKDSRMKAVDEALNYAKQIKLNTLEEFFEQRIRDYRERELQQLKNEVMMMIFLPLIQSLVAILTWEAVFFLTNPIDFSIINIMMSNYSNISNILSNLPYQYKSFQVSRNSMARLDNYFKQKECNMIERQSENNNFAIKINNGIYSWKSNDQMNQDVVEIKDENEQINDQDIFNIDINVEIKKGEYVAFVGNSASGKSTILRSILGETDKKSGNIIVNGTISVATQEPWIISGSIKKNITFMNSFDALKYKQIIKICGLERDISSFKNGDDTILGEKGDNLSGGQQKRINLARAIYNDADIYLLDDPTSALDIKVKYQIHQQCLSGYLKNKTRILFTNSISNLQDCDRIYFIENGRIIKESKIPQTIISNGDQKSSKQEIIETQIESKQVKPEFIDNQEARQSLLQKEDQEQGDISLKVYKQVFVYIGQYFAPLCTLVYFVIVLGFQLYGNDKLAQQNVSNEEYKQIAKIYYPITQLPVSLAVVLINAYYLLMGLSTSRKIHSSIINRLLNASYTKFYNTILIGRLMNRLSKDIYNIDLEFPNEVQNFSIQIFNLIIPLVECFIYLNIAALPLLIIFFIGLIYLTIIYYRCLREITRIEAVSKSPIFSFFQQIVRGITYARTCLPLEKIIQIWEIKLIQMGFNIGINLWQDHXLSGYLKNKTRILFTNSISNLQDCDRIYFIENGRIIKESKIPQTIISNGDQKSSKQEIIETQIESKQVKPEFIDNQEARQSLLQKEDQEQGDISLKVYKQVFVYIGQYFAPLCTLVYFVIVLGFQLYGNDKLAQQNVSNEEYKQIAKIYYPITQLPVSLAVVLINAYYLLMGLSTSRKIHSSIINRLLNASYTKFYNTILIGRLMNRLSKDIYNIDLEFPNEVQNFSIQIFNLIIPLVECFIYLNIAALPLLIIFFIGLIYLTIIYYRCLREITRIEAVSKSPIFSFFQQIVRGITYARTCLPLEKIVIQQQINVDTDLGNQINLDGIQYWYQSLAGSSTNVFQALLFIICFFFPGKNSDKTFLVLNKMQEISLLVLNASISYGNIQMYLISFERCLHLANNVDLDERFIPQISINEEALENKVTQNTVLNLENCTFQYRPFSKSVLQKLSFKLNKKEKIGVVGKTGSGKSSIILALTAILEQIEGSIEIENKKIDQYSIKEQRQKFGIIPQDPLIFMGSLRQNLDPLNKFEECYIQEIVKQCGLLEMQSFKQQGLESEIALQGSNLSLGEKQLLNIVRCILENKSIVLVDEATSNIDSKTEEHVKNLFNKYFQNVSMISIAHKVTTIMNSDRIIVLNDGQITEFDHPSKLLSNPNSEFKQIIDLIKHSDNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJG1</accession>
    <name>A0A8S1SJG1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ammonium transporter AmtB-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0100160</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJG1"/>
    <dbReference type="OrthoDB" id="534912at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097272">
        <property type="term" value="P:ammonium homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3430.10:FF:000008">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730:SF6">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="221"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="250"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="284"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="307"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="339"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="377"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="10">
        <location>
            <begin position="34"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8141511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="443" mass="47706" checksum="D892A15501768BE4" modified="2022-10-12" version="1">MQQHYLDHTNPANPPPAPNPAAGITNGSTLDEDWLLIAGIVLAFSQLGFGFLEAGSVRYKNAQSIVIKVFLGMCLTVLIYWLFGYGFSYGDDFGTYFMGGTKLGAYNWAATEAQNDYSNFVFKATLSCIGVSIVSGGAAERLTFLAWGVLALFYSGFVSPAIVHWTNANGWLTKLGYKDLAGSGFIFYSAGVAALVVTVVSKPRKYRFDPNSTLNFQPFSSIYVAFGTVILFATWMFINGGQIQSGQSTPYVQGLVAVNTLIAGAAGGFWSFVIRYLTKETTSLFSISRGILAGLVASSAAAHESQIWATSIVASIAGIVYTLTATALPKVKLDDPVQVVPIFLHGGFIGVLFTGLLDVNGGLFYGKGMKLLGCQLLGLLIITVWVAFFVFFILIVLKGFAVLRIDSDAEVAGIDKEKCNYEAISFSNEDPQQQVNIIKSVSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SKK1</accession>
    <name>A0A8S1SKK1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0080334</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="292001at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8139867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1389" mass="162864" checksum="DBC3FA3BE34A20A2" modified="2022-10-12" version="1">MNLIDSLSNEFQQLLSDQKKNQVLKPLIQGIIDDIKKHKSQQNLPIQEIIKILVRLPESKTNQTSGIALNIILKLFNTPILQEADLHPLLHSLYLFKDEIIENVQLKLVQCSVHLIHKEIVNINNPECIEKLICIFFTLVISKNPIIQTTSMTGLMKILDFLYAQYSEKPHRNGVLIFLQLIQGMKGQKVPFVVQHQYSKGICRDMIFQIVQNLGPFLNKDEQLSKLIEEISAIIYEEMSLDSADVQFNARRIRDSFRMIVSLQRDISLLKHISLLFTRSPSYGYVRYWILEGILTLLQDPKLVMLLYQGPLQEEQQQQQQQQSTFLQHLVFIIKLSTEQEPQYTINQSNQQQTYAKQKKLYEQNILNLTEIPIYNKQQFSQKSIECISHFTDSILQIAYEKQIGLFSLNYKLSQSKILIEDNCKEVNQIIETTQQLTLKTIQNLMINLNDEIQYQSILNSIQTWINLSGSLGQNKTRDAFIKYLSSLCVAKQNTTLTKNQLQSAKTLFNIAQLGNLLDIKSWYIIMKAMQQFEALLQKSQTQNAIQQESHPEIYQQEISLLNNILDGLFSSSNVYEDANLLHMIEAINQVTLSLMEQFNNVQTLVDSKSIQFGLQKIHQITKQNWFRINKFWDFITAHFLCIANYKHKAFRESALEIFSYIVQQGFIYFLKPDQSLCWEGDSWQSHLLSPIQQMINIPYADVKETLLNIIFKLIQNNGHELNILGFNTIIEILLISCDETEPTGYVNIGFHILELLIGQFMHLLDPKTTRRLLPLIKQFRQRTTEQNISYVSVGLIWQLADNLSKICTNQTSQTEVEELWTVVLQSLKDLSLDNAPDVRQSALHIIIQIILINCGSFRINFQIDLLKNLIFKILDDLIGRVAQLQQLQVIFHMKVPKYLKEEDYPKFTKFSEKDLQTTIKPQNEIMVAWEDTLKIMIQNLTKFLKKIGQQEDQEFKQHAQQLYNETFIRLIISFKINNLDLRWEIIRLIKENTEIMIEKNLVEQFDWAKEFILCIQEFIGQKIQDNRDVKTLINKILPEVCELYVLFLKAHNKNQNSFPEELVDDLYDSYQAIVDLPINIENISNIKIWMDEKQLHDFAEDFYVHLSNQKDKSKFYSFLLLNMKKEGGMTKLQDLVILKYTQLLSKYITDKQLTDPELIKQYIDICLLQLSIRQNQQKVNNVKNYNKTNKPLWVQVLPLIIETIQICQKNEYVQLLSEQLNFTQQWDKIISIDGLKSCIDVELQICEWLLSQDILDREIINYWELLSRNINLYQLEDLFYETRQKLMNIIFKKPKNLMTLNQICQSLVTQFMKDEYMSGSMPLSRKRVQEIIQLLLELQSLKDLNLEPHPLLQVFEFLVELITTREIDIKQPLQNLFKSVALKIRSHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SKT8</accession>
    <name>A0A8S1SKT8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0100008</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SKT8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8141235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="42" mass="4925" checksum="F3522185E8AF35A3" modified="2022-10-12" version="1">MQTIAFFKDEDLKETFANQTSIIQTLYHAQLEVSKINENLHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SNC4</accession>
    <name>A0A8S1SNC4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0080372</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SNC4"/>
    <dbReference type="OrthoDB" id="300654at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8139942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="116" mass="13536" checksum="F26A57C47870D368" modified="2022-10-12" version="1">MSNKNPIMQDNAQTNNSLIKENDSKVMNNKSQVQQLTEEQKQQQMDQFKKFLQQTGISDAFQVIFAEIIDKKLKEEDSYKYTADRLREIGKGLSSEQMKAKAEMQKKELAEKMKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SPJ3</accession>
    <name>A0A8S1SPJ3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0090010</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SPJ3"/>
    <dbReference type="OrthoDB" id="10355204at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035796840" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8140312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="274" mass="31127" checksum="CA12BCA29C7EDD64" modified="2022-10-12" version="1">MRITIVFLYSVILSLAQEDFPGESSPLQVEVQIFGNSEQTKIKDEEAGIFQFDDFKRGTGSLNQFFIKDLLETISAAFESIADDKDDATFLQEAQKLPSQSDGLEPIQVESPIFGFFNPFISIFQPSEISDFVEIETISINGQKQTKVTKTQTRNGITTTTTEITRENTQNTNSNQLSDEFLLQENPVMEEGEKFIESEEEILGDNVNQINLDELESIQDFHLVQDNYVKNEQVMNIEEKQVQQLPLGLTIFGGFILTLFIGYSIKKFVFKNNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SPM9</accession>
    <name>A0A8S1SPM9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0090072</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SPM9"/>
    <dbReference type="OrthoDB" id="310740at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23524:SF1">
        <property type="entry name" value="MRH DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23524">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G04850)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="332"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="364"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="389"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="424"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="458"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="3">
        <location>
            <begin position="287"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8140434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="484" mass="54072" checksum="59D79C7065B3E070" modified="2022-10-12" version="1">MNQIEPTDNGAQEKKVFKGMIKLYPDISQTNYLCSLCHYFVMTFIFVSVESLQPLLFNQRYGIHPKDAGMQNGYMLLIDIATKCIFAPIVGIMSDKVGRVPILQMGIVLTALSITSMGFAENIYPQYCISRIQYAIGAITLATIPFLGDYVLDETKGKASAINVILAAFGAMFSATVITKLLTQSFSIQTTYIVVGSSFLCLGLLYTLGLKKGLHFKKERKIYKNAPLRDDLIQKSICPYQNIKQIQDEDFDYIDYDKEVRALNQIKQDSSCGAAMKIALLAGKNIWIFQGYVTNFLGRGDSILLTLSLQIWSQQFVDETLNDDDAYKQASIQAQTLSGVTYMVIMIGAVFYGILFEKVSKNKLLFIMFSLTMVGCFLMNFAPNPKSPYTFLIMSILGSGMSGLYTGALYFVNKYAYSEFRGYISGLANVFSVLGILICSFVGGVLQDHWSKIAPFNLFGFMSLLGLILVVWSYYALYQSRSVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SRB3</accession>
    <name>A0A8S1SRB3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0120147</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SRB3"/>
    <dbReference type="OrthoDB" id="309786at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8143315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="156" mass="18705" checksum="831653F9D1736712" modified="2022-10-12" version="1">MTEKYWQRYKETHDKDPLNTNELWVRVKQDLKRREKPKHHLELYSYCRTENPYIKQIKSSPNITMDTVAELICRQSACELQYCMSLQKLAVENSRSKIDLQGCRDQYKTMTTCIMNEKNRIKSKEEELRNLVLGLQQPEIKQEPQKQPILKEEPVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SXD5</accession>
    <name>A0A8S1SXD5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0130378</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000013">
        <property type="protein sequence ID" value="CAD8144743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SXD5"/>
    <dbReference type="OrthoDB" id="1060944at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16306:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_7, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16306">
        <property type="entry name" value="TRANSLIN-ASSOCIATED FACTOR X-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="753"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="323"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="459"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8144743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="837" mass="98981" checksum="B4DFB656595A074B" modified="2022-10-12" version="1">MNQKEIAKEVLAVSLHIADADKFDFEDSKKRMQLLTESVLKTNLLRQKPNKNCNQTVDLEFQQIDNLKDLLPHLAECSNLRELILHGNRLHTLPEDLSTLNTVETLDISNNLFDDIIPIINALKTMPKLIHLHIGLRNINEEKYLILQLPNLITLNNRRLKFFDQLDSDRPSERQSARSEKSYQSEITFNQDDVDNVENLFNQICNMENNSNQQKTHSSQLTDHVKDVMTDFSTKVQQTHSKHFLDTHILKAKYDLYEICFRELIDYFRNNNNKLEQVLRTLQQEHSYIFNDLTNIIYNLKDYKKIQKDNHEIEQLILNSDIVKSQNKEIQLFKEEHDRLLNEKNKIQQQKDQLYELIHPLQLQINTLQNTLQQLEQEKENLNNINSQLEYQFAQQQLLQRSHRDSSKQIQSANQSFQDIQSFSNFQTQQVVMLIKTTQTDYPQFFEKESQTNDQEGKIINKSIDLQSFQQELEDLQCQNEQLQQENKKYLEMIIRRSKVENSSNVMSDISYKQTESPSIIEKKRQKALSEFHLLVQPQSFRPLTLKQLKDIIQDIYESKSKHDIRQYENKLPLETIEQHIYTYLYCKYGLKNLALEWVACIINAIKKYITEDNDVTVFGLMLKNNIDEDFRILQSQRKLNIEHIIQNILQAKYKTKPVAEIKEMVKQKVAGQITQQEAIEILKCCLGEEDRETILNLLNMHQIKTATSVSPIKRINQKEQQRRPSAQKEQIKYEFSIFIKLILEYFMNLYQQYLANLCVVFKSVDHDKDGIINQQQFLKLTESIKPNLKNEECSQIYEILDPFKNDKITFSQIVRVFKNPQLYNKNVDLIQQISIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SYC5</accession>
    <name>A0A8S1SYC5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0150422</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SYC5"/>
    <dbReference type="OrthoDB" id="309101at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8146591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="106" mass="12517" checksum="53264B246FA74605" modified="2022-10-12" version="1">MKSGLYEEDNLHPQNQPGFITITIRTTSDIYVNNTDIKINPQIPFCEFCRIVKQMIFSSVNNKQFLHEILFLKKFQEFRETEVRSLEQLGFRNHDILDVKLRLKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T0G4</accession>
    <name>A0A8S1T0G4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0150044</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptobrevin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8145843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T0G4"/>
    <dbReference type="OrthoDB" id="312685at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14824">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15843">
        <property type="entry name" value="R-SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.50:FF:000015">
        <property type="entry name" value="Synaptobrevin 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000004">
        <property type="entry name" value="Vesicle-associated membrane protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051097">
        <property type="entry name" value="Synaptobrevin-like_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136">
        <property type="entry name" value="SNARE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136:SF168">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13774">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01270">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="195"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Longin" evidence="10">
        <location>
            <begin position="12"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="11">
        <location>
            <begin position="131"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8145843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="228" mass="26347" checksum="35A0F2E08F7298E5" modified="2022-10-12" version="1">MQSSNPQIIYAVVVRGSNVVLSEYSIAKGNYIAFAKTIISKVNQQNAKKSFNYEQYEFHILVEDGFSFLIMAERGLKMRIAFACLEDMKQKFFQMFQPQQRDQAISYGLNSQFSIEQKNKIEYYNSPQADKLRLVSDNIQQTKEVMMENLDKLLERGEKIDILVEKTNVMVNISTSMKENATTLRRQMWWRNKKMTIILVLVGLLAIYIIMVIACGGFAMHRCFGSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T1L1</accession>
    <name>A0A8S1T1L1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0150252</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T1L1"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF13">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="6"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="355"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="355"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="369"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="396"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8146253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="522" mass="61641" checksum="3F26A4FC22F9867C" modified="2022-10-12" version="1">MKDKYIVEENVLGQGQFGSVQTAKSLENKQLRVCVKIMQKKKFYGQNVLQTVQEEEFNICKKMVNKKFDNLVQIYDVYDQDYNDTQAYIFMERCDKSLKELINLKKSGQEKKFTKEELIDIINQIAKGYNQMISQEIIHRDLKPENILCNIVDNQIQVKIADFGLSKIVDSPSQKKMTKKVGTMYYSAPEILNEKNPDDYDNLCDIFSLGVIIFELALLQKQYNYKQIEKLKETTFSKLHEDVIQNKEIDKDILELLDKMIVYDPKQRLTWQELLNYFDQKPKQSSLQSDLQQQQLKRQQNQQFQNQQQQQGQFQCPLYINAQKQQNGLNQQPLQQFLPNPGFQQINFQQNQFPQQNLPQFPQGNQGQSALQNQTQNPTRYSTMSKAQSLPEQSSQAPNVQTQAQPTQQQPQTKFQFTQPINNLNQQNTFQQGGVQFNQQQKFTQQQQFTQQQLFTQKQQFNQQQQFTQQQQFTQQQQFTQQQQFTLQQQFNPQQQFNLLQQFNQQQPQGQPNRLMTMASKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T4G5</accession>
    <name>A0A8S1T4G5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WD40-repeat-containing domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0140397</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="538223at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8145724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="589" mass="70459" checksum="99E6D5E712757846" modified="2022-10-12" version="1">MKELENQTTKVLNNQKSVQNLLKNYGQFNRLFDSLIRIAENWSKDLELQIKECYKYKFDDELDDYMKKQNNSEIANITLMKNINRSWMKKLYNNQNQYNQNEDKINFRELKKQFMRFINKKESQEDEIKFNLIDQSVKYTIKCNAISFDSSGQYMISTEYQNIKLWSFKNGKIKYLQIFYGHSKWIQQIKFLYFMFWRQKFRCWKNQNFNDWISLEPYKKHTDWVMCLVMNKNEIYSFLEVVIIQQKYGSLILIKMNQHFFIHQINIIIMLFRQVQINQRIISIMCIREELDYYLGKKRKNTYEFKYFVRIPFIYKQSIYNYRLKDSFITGNSFISTTGDQGIDMIYFELIEGIYQEIQHKSIQLMNNDQDYDEYFFSIIYNKQRNLILVRHKSQIYLIRNINNGNFKIVDQLNCNTSDIYWTITDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXMQYLLILLDNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T683</accession>
    <name>A0A8S1T683_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mini antigen</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0150195</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T683"/>
    <dbReference type="OrthoDB" id="300631at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002895">
        <property type="entry name" value="Paramecium_SA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01508">
        <property type="entry name" value="Paramecium_SA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035911702" description="Mini antigen" evidence="1">
        <location>
            <begin position="17"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8146142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="266" mass="28852" checksum="162BBA717C3192EC" modified="2022-10-12" version="1">MNKMILVIALISITYAQLTFNANNKCTCGDLTTQQDCAQSSPICVWDSKGSSCSTVSCSSIGDQTSCAKNLKCMWNGKGCVDFTLCNQLTGVNQAECYAQSNNCPQSDGKNCASKEALKSCSSYSNQTSCDLTISSNGICFWSTSGCSEITSCGQLNPNTCSSIGNACQWNATISNCTQASCAQFGNNYTCTYFQTQLNKFVYQLCQWNSANNNCVAASDTSQLNINTCYNQTLQTYRWISSSNKCDVCAGKIIYIVIMAIFMIMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T7W4</accession>
    <name>A0A8S1T7W4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0160024</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8146732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T7W4"/>
    <dbReference type="OrthoDB" id="298080at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035818433" evidence="2">
        <location>
            <begin position="18"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="247"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8146732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="363" mass="40719" checksum="5814F81EE89A106C" modified="2022-10-12" version="1">MARSLTILAIVIAVATARLTKTESPKEILAQVNKDSFGNSILSVLQLQLATGGPVGEIQILLNNIASQLNGDQKKADKVHESDTVAFEKIIADLEQEIAYHQSQIVALSNLRDSTTEALGEAEAEVRVVTSDIANNEKSFADESATRQSQHETWVRKDAEHVDQMDAIDEASKIVQHLQAGVAFAQLKSRFEKVQAKLMESKHALFKPLINALTQLASKVDNKSIIKILELLAQIRQQLVASRASLLATEERQAANWEVQSSHLQEEHKRLVERKAFLENSIVQFKVTIQEAVEDLEDQTLFLEDAEDSLAIQERWAAEQEAQYEAQTFEREQQLEVVERLQEVLTQKLSAASEFLQIREEVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-06-18" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T8I0</accession>
    <name>A0A8S1T8I0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0170303</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8148108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000007">
        <property type="entry name" value="40S ribosomal protein S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_uS12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00055">
        <property type="entry name" value="RIBOSOMAL_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="275"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="108"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8148108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="312" mass="35939" checksum="7ECCF5DC87D4D0F1" modified="2022-10-12" version="1">MGVGKPRGMRAGRKLKDHRRVNRWADKEYNKAMIGSRYKNPFMGASHAKGLVVEKIGVESKQPNSAVRKCVRVLLKKNGKKISAFVPXXXXXXXXXXXXXXXXXXXXWINELMQANKEIRNQEQQMRIQNKQLLEDLESWKSRYKLLQESNSKSSGLDEEIRQLKKKINELSEDLAGSHEQIQQRDNEILNLKQLLHDKDNELDQLDARIRELEMMCENYSQSETQIISLQGEVELWKKKFKQVNEQNSDLAEKLTMAETQLEAIKKRQVTVTKETEVRKSGTHGGGTTSTYEQNVIKGAQLLHPRGSQYLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T9E8</accession>
    <name>A0A8S1T9E8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0190368</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T9E8"/>
    <dbReference type="OrthoDB" id="317926at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706">
        <property type="entry name" value="MORN VARIANT REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33706:SF1">
        <property type="entry name" value="TPR REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8149895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="128" mass="15326" checksum="999EAED22C7D6522" modified="2022-10-12" version="1">MSQFVDYEWKKECEGIIWDYIYNPPRKIRTKYLISRTNEKEIMYTLDGCSLKIDRIIGPSKELDLMNNLEQIKHLQWIGQYGQNNVKIGKWIIRWNGETLKDVGGQYSNDGKKQGRWIELFSNYWNKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TAD9</accession>
    <name>A0A8S1TAD9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0170016</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TAD9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8147542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="111" mass="12887" checksum="E33B2F96577BA7E3" modified="2022-10-12" version="1">MISFAQDLNINLITFTAYGIPCSSITSNTIPNVPEHKYFVITYLSILSPITQWKWHPLLEELPDELLLLDELEEEELLLLPLLLLSVVDLAINLLQIAQITLVWKYHDFYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TAI5</accession>
    <name>A0A8S1TAI5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cytosolic endo-beta-N-acetylglucosaminidase TIM barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0190115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TAI5"/>
    <dbReference type="OrthoDB" id="284473at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033925">
        <property type="term" value="F:mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000355">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032979">
        <property type="entry name" value="ENGase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005201">
        <property type="entry name" value="TIM_ENGase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13246:SF1">
        <property type="entry name" value="CYTOSOLIC ENDO-BETA-N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13246">
        <property type="entry name" value="ENDO BETA N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03644">
        <property type="entry name" value="Glyco_hydro_85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytosolic endo-beta-N-acetylglucosaminidase TIM barrel" evidence="1">
        <location>
            <begin position="251"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8149450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="960" mass="111632" checksum="30727E04E1C887F3" modified="2022-10-12" version="1">MSNYFLNSGLKRDGQLKLVPEHVRFNLTQLTPSDVGIIQFQLILAEQEQNIIELIDIHDNLGNPLIKITLKQRELLIQYLDNTYKFGIQQIILYKLYPIFISFHDEISLITNFSIQSQKLQQRFTYTSNKVGIFLGSKSNLNAFTGITRNLQIRFVRENELKMQMEGEIKIQKSILNDLNDIGQIKYKLPFSKPLQSIEELDNWKPNEEYTILPIQQLKKITRTTNQPLLVCHDMQGGYKEDIWCFGNPMRQNSYRFYYMTHCDIFVYFSHTFITIPSVPYINICHQFGTRILGTIITEGEDNTLTNQILNLQYVDKLVQICQFYKFDGYLLNIETNVYNVDLFIQFIKELSDKLQQIGAILIYYDSHNAEGNLKWQSELNQDNMKYFQACNYFFSDYHWNLNKLSNTEKNVGNQRNNVFVGIDIWGRGQYGGGQFDSYIALQEIKRAQLATAIFGQAWTYETSNDSRSQFIQNDNSLWQGKNTVSLFDKHKNQWAITFNGGNGWKIIQENGEETAISSFDWCVRTYTIQLNLVSSNKGGIIQFNAKVKGTGPKFDDLYLIGIELYDRNNRCIASIDSFSTKVNKREIQFNVDSVNIADDKWREHTLSIKTTDDTQYVKFIEVGKDVEYWAGNYGTQFTGESMVYISYDSNKDLLRMLKPKTYQEFPLQTYFNNAVGEAYYIDGQKNSHYKGYYDNLNDFDYSLCYPTKKIISQDIQDNWESSINFSDSWNGSSCIKIKGDLQVHKSFLIKLYKTRIDPQGNINASINMKYFDKKILQLSLVMKFADKGLQIFKPTKILTNKGWDIAQYTILNQGKSLKSVYLKIENISQQQQAVDGLIGGLAIYDDKYTEQMKSANVDFKDPTKYIKNIQLKESITNLYDLYIQLELGELGPLLKTIRIFNDKQWIGNVRSNYAYFPELPFDGKEMFVRLQIVLNNGNHIKAESVLPYILYSDQVVRLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TE33</accession>
    <name>A0A8S1TE33_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">OTU domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0200109</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TE33"/>
    <dbReference type="OrthoDB" id="18915at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071108">
        <property type="term" value="P:protein K48-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22749">
        <property type="entry name" value="Otubain_C65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1300.20:FF:000006">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019400">
        <property type="entry name" value="Peptidase_C65_otubain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12931:SF15">
        <property type="entry name" value="UBIQUITIN THIOESTERASE OTUBAIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12931">
        <property type="entry name" value="UBIQUITIN THIOLESTERASE PROTEIN OTUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10275">
        <property type="entry name" value="Peptidase_C65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="OTU" evidence="3">
        <location>
            <begin position="150"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8150138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="350" mass="41979" checksum="234B90A126D0D101" modified="2022-10-12" version="1">MNIIKAFIIRNYRFNKPINKSYILFGIVFAIGFAKFLNDKNQKKKNSEILKNNDQKNEQNNDQHNDQNNDQHNETVNENKIQKIIKPQVQLQDIKKSQLLDQIYDQTLRARDVPEEFKSFISDEKQLDFLLDEYGMNNEKIQEQLTNHLKGMRRARGDGNCFYTSFGFQYLRYIFIKSSDQQFEDFIKMVQQLPFSVIHSDQMYEQNEEMNQIFIDYCQYIRAIDLESREQEFIKLFSNPEGQFYGLCIIFLRNIIYKLCLEDEETKMVFEVIEMNLQQQILTWEFDCENNQSIINLLSRKLLIDIILYYVDEDARVLKVQEYKNENEQILLQVNLLFRPGHYNVAIPNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TEN1</accession>
    <name>A0A8S1TEN1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0190076</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TEN1"/>
    <dbReference type="OrthoDB" id="284174at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8149372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="100" mass="11456" checksum="FBD2CBBB702A49A4" modified="2022-10-12" version="1">MGYFDLINQPLNEFGEELTRLKTDIVTNLKPESRFNVLVGLSVVNLAIGVAVKGIKSRPLQTSFISFFGFGLIIYPELYFGLIRQKKVQQQITHHFIKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TFR5</accession>
    <name>A0A8S1TFR5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transketolase-like pyrimidine-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0200289</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alpha-ketoglutarate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000020">
        <property type="protein sequence ID" value="CAD8150494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TFR5"/>
    <dbReference type="OrthoDB" id="413077at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016624">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011603">
        <property type="entry name" value="2oxoglutarate_DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031717">
        <property type="entry name" value="ODO-1/KGD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006914">
        <property type="entry name" value="PRK09404.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152:SF4">
        <property type="entry name" value="2-OXOADIPATE DEHYDROGENASE COMPLEX COMPONENT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152">
        <property type="entry name" value="2-OXOGLUTARATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16870">
        <property type="entry name" value="OxoGdeHyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000157">
        <property type="entry name" value="Oxoglu_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="5">
        <location>
            <begin position="546"/>
            <end position="742"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8150494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="893" mass="103730" checksum="11815036F587B369" modified="2022-10-12" version="1">MQQHFNYARLLKYPFSNYKNYGQLTQVKLDKSLLENIENKQYHFIQYYRQNGHLVAQLDPLQLIENKQKEMPGVLDFGIKYNDDIKVNNILKRDLKSIEELEEYINKLYASSVGVEFEQIEDPEEKQWLHENYEKFINEEITQADRVSIHNLLVQMEAIDHYYHKKFTTFKRYAGEGGEGVIVALRAIYGQAVELGVTDVVQSMAHRGRFPIMSSLLDYPPSDIFRKIMGENDLPQEYTFGVDDVVHHLSTSNKKKFNNKDLTITVVHNPSHLEAANPVSQGKAKAKQDDYGNVNQVLNLQLHGDAAFAGQGIVYESMLLSGLDNYTNGGTVHIIQNNQIGYTTNIKDSRFSRYSSDLLLAYKYPILHVNGEDVETLHKVSKFAVEYRQKFKKDILIDIVTYRKYGHNEVDEPSFTQPNMYEKVRKAKSLPLKYNQLYFRQEDYEKIRQKVFTYLDSEYEKAKTMKQTLAKITDEKSKGSKAFTQKWSKMKFTQFCEQDAKTGLNKDYLINLAKQSVVIKPNFNLHPRLQKYFIDDRLDQIQKNSIDWATCETIAVGSLLEEGYNVRLSGEDVERGTFSQRHWAFIDQKTEEKWIPMQNFADAKLQGRLQVANSPLAEASVMGYEFGYSLENPNNLVMWEAQFGDFYNTGQHMSDTFITCAEEKWMRQSSLVLLLPHGYDGAGPEHSSCRMERWLQQGVQNQNFQFAIPSNPSSIFHILRRQMHRQFRKPLIIANAKSLLRSNKAKCTFEDLGEDKSFQRIITHKQGENPNKVIFTYGKYVYDLLEQLEKKNADNYIIFTFEQLLPFPKYELEQGLKQIKKDVKFYFVQEEPQNQGGFNYCQLQIDQVLSTLQFTNKTINYIGRPECPSVAVGASILHKQELQNIVKQIDSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TJH7</accession>
    <name>A0A8S1TJH7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0210191</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8151139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TJH7"/>
    <dbReference type="OrthoDB" id="300740at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="145"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8151139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="254" mass="30261" checksum="6D03ADF931D5A28C" modified="2022-10-12" version="1">MDSLNSKDDKFTTMTDSAQLKSFILNKNINIKEKSKFVKIIPQNLLRISTMHQLKKQQTLTLPTDRSSNLSRENEQFNDKFLVENIEKKQQQLKHKTSRFKNLLNGRRIKTSGDHFQDYKEEYKKVVEAGYNEIEENFQFCQASLHDQRQNLMNAEIELQENQKELELLQDQISTLKEAIYLTKGQSKKSLNDILFRNRLRQNKERKLTQELLEFVNHLSIQYDYHITSLKEYLQSFKTELSEILIENEYTEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TVG7</accession>
    <name>A0A8S1TVG7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS17 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0280190</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000028">
        <property type="protein sequence ID" value="CAD8156210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TVG7"/>
    <dbReference type="OrthoDB" id="299793at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00364">
        <property type="entry name" value="Ribosomal_uS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.1000:FF:000001">
        <property type="entry name" value="40S ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_uS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032440">
        <property type="entry name" value="Ribosomal_uS17_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744:SF9">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16205">
        <property type="entry name" value="Ribosomal_S17_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS17 N-terminal" evidence="4">
        <location>
            <begin position="5"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8156210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="159" mass="18151" checksum="5037BB5669C01F7F" modified="2022-10-12" version="1">MADVQTQRAFQKQEGIFQNSKKLLAKKTSKGVRYWKEVGLGFKVPKDAVEGNYIDKKCPFTGNVSVRGAILKGIVISTKMTRTIIIRRDYLHYVAKYNRYEKRHRNVPVHISPAFGPVNEGDIVVCGQCRPLSKTVRFNVLKVIPNEIIGNVRKQFVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TZZ1</accession>
    <name>A0A8S1TZZ1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0310037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8157858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TZZ1"/>
    <dbReference type="OrthoDB" id="306221at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="135"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8157858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="294" mass="34671" checksum="02AFDC8F15072184" modified="2022-10-12" version="1">MKSVSAPKYNYYISLNNSFSTPDTKINKSRQNTQSTLKLSGEMMSVQSYLYEQAHLKEEASKKEILRQIRDVIDNLEKNKSFKQLNQQLSKLIIGLRTCINQQEVSSYIIQLIQIIREITNKHQHQKQIMHDKDKANLMQKIHELEQQAKENQFQMKLQIQNKEAEERKKHYEVQLEQIKQIKNQNQIQPKKQQTNTKEKMELKNQIILMQTKIQTLSEKEKKLISLVKAVKSRGIDVEHIFKNLNQVSSRNSKITNQEDKEEFFNSSNTDFADISQFDIGQSSIKRNQKFLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U2U1</accession>
    <name>A0A8S1U2U1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Actin-fragmin kinase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0310281</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U2U1"/>
    <dbReference type="OrthoDB" id="298229at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05124">
        <property type="entry name" value="AFK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015275">
        <property type="entry name" value="Actin-fragmin_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037469">
        <property type="entry name" value="Put_AFK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38737">
        <property type="entry name" value="ACTIN-FRAGMIN KINASE DDB_G0279609-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38737:SF1">
        <property type="entry name" value="ACTIN-FRAGMIN KINASE DDB_G0279609-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09192">
        <property type="entry name" value="Act-Frag_cataly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Actin-fragmin kinase catalytic" evidence="1">
        <location>
            <begin position="32"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8158322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="373" mass="43768" checksum="878F05A74995FBA2" modified="2022-10-12" version="1">MKQNKYIKNIHLRSKEIVEQQIEQQNVNKSQVQLQEFDYAAKPYVDFDFIKLKNIKSIKMSDSGSRGVIFIDSEQGAIVLKLSGQVGVELFLNKLAQALDIKTTQMKCLKWCDVEMQELRNDILFAASTDEVLSHRLKQKLKVAYFEIIEYIPGLQLYCFQGERAKKIFNQERLFNLGKIIGFDIFIHNGDRFPLPIWRSVGNAYNIILKVIDEKQEDMFNIHNANLNFDCIYSIDPSTILKQLDSSIQDKILNTYIEKVQKFLQELCDDVKKNESKCLEAFQDFIFEQTQYKLNDNELQIVKKGILYQIQKISQFGIENIIKIKQELIVPDFQDWMDSYNNCLNQIHIEFHEKLIKVFTEIINTNSELFQTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UHU3</accession>
    <name>A0A8S1UHU3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0370273</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000037">
        <property type="protein sequence ID" value="CAD8162246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UHU3"/>
    <dbReference type="OrthoDB" id="307362at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.280.10:FF:000013">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003428">
        <property type="entry name" value="MAM33"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02330">
        <property type="entry name" value="MAM33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8162246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="236" mass="27730" checksum="696DECF4BE7019A5" modified="2022-10-12" version="1">MITKMPLRIARTLFSVNFPGSQIIIPQQLIEINHLFQLTEIEKMIETIPLNINHYNNDQIKWNGFKFKDFEDQLRIELSKNVENIQILICIDFQNRQQSKEQEIIDNQIIQIQNSSENYYSSFQSNSSPFSIYLTKGNGIISCYECLAYNGKIIIEMVSIIDDIEEHKQIPRQNRGLKDYNGSNHLIDESIQIDMLNYLKTFEIDSELAQFVQHVQIQKDQVLNVIYLQSKEILHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UTF7</accession>
    <name>A0A8S1UTF7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0490166</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UTF7"/>
    <dbReference type="OrthoDB" id="307182at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="381"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8168538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="514" mass="59986" checksum="8EB73F6E054F219B" modified="2022-10-12" version="1">MFAKIAQHFKSLTMKSQIILFNVITLVIVLSIVWINYYVQTEIFSAISSSSLIQILQVQDQDQIGFIAQNVAIIIELKFITVLQQIEQIKQFYNFFDIEKENIVNNYKMNPCISMKNFIQVKKEFQTPKFCYQAIGVPDYISIPQDEKEVLLLHEFISFLNHYSLVINVPFTGKLQIASISQTKYFAQFPSGLLFPSYDITQRFWYQNHLLKTEADSNTGFVFSPLNEAFASQTKEMSITNSIYKKDQMIGIIKEGLLVNNQLIPAVPYNVLLLDQEGLVVYFNIEKLINKTDYFYIYDENITGFNKTDWEEMIQFSNQRERVILVLENKLQKEKVNIYSLEVLKNNFTLIVYTNITTKIDSQNKSEAIREKSFFNFTISLFVQIMLGFIALIVQIIVILVVFRPLKQFNSIIKQYTLSQGNNINSEIFKMINKKPQESDALSTLQNKILSFSQILSESQGRKCEMCKIWEAFSYNLNDPEFDFDLFKNQFKYLENGKQELNKKMLKIIKQGIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UTR1</accession>
    <name>A0A8S1UTR1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Kelch motif family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0430161</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8165856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UTR1"/>
    <dbReference type="OrthoDB" id="4447at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23244:SF471">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT BETA 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23244">
        <property type="entry name" value="KELCH REPEAT DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8165856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="562" mass="65942" checksum="6FEFAA738077E9A1" modified="2022-10-12" version="1">MNYDSKRIETNYSPYFEQVSLNKLKRDEYHSLQRIHDNNRKNKLLLPSIENSTLEDISMHFSKCVNSSKSPEKKVDKLQVNKYQLEFIQQSFLELEQTKMSITMRDMNNVRNKFNCHQRIIANKELYTVRNLEKQMGSNQEDYSNVMYLQQIPPQTKNKQETNMAFFEETLNAYSYAIPSRRESAQIAVIRDKVYVFGGMSGAGLSSDLWSYDIKKQEWQLHQSDINLKVTNHSMIAWKHMLIIFGGSGYYDHKMKIRQVYSTLAYFNTQTNQWSITLESIEPRREHKAVLYLGKYMIITGGIDSGEQLLNDTLMYSLDSRRWLGSKIYFDEGIAQHAICEAFDFKRNVDTIYLFGGKTKSLGSFPLMRLVFSGQAPQSWERVQGTGVSPQGRYNHTMECLNDSLILIGGRSQAIQEYQNEILVFNLILSQWIQVKRQGLMTKRWSHCSCIFASNIFCFGGIGEVTYLPPVVFSIETDQFKIKNKMVIIKRLSTIAEDMKKQNQEYKQIKRTCRLEKFRKAEKLYEKVTTFLPLPKMRTPKMRYDIWMQFIRKILHSLNLIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UVZ5</accession>
    <name>A0A8S1UVZ5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0490044</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UVZ5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035716679" evidence="1">
        <location>
            <begin position="21"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8168353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="90" mass="10221" checksum="E5B0441A67BAA673" modified="2022-10-12" version="1">MKFFVFILLLGLVICDQLHAQSNLRYEYQFAPWSLDCTNKCQSAGGVVCGYETQVCCRSGKCLKHWKSETCDTWDKIHILNCVPGPSSKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UXA2</accession>
    <name>A0A8S1UXA2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RanBD1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0510009</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000051">
        <property type="protein sequence ID" value="CAD8169361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UXA2"/>
    <dbReference type="OrthoDB" id="2357150at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006913">
        <property type="term" value="P:nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13179">
        <property type="entry name" value="RanBD_RanBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045255">
        <property type="entry name" value="RanBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045256">
        <property type="entry name" value="RanBP1_RanBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138:SF87">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE RANBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138">
        <property type="entry name" value="RAN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00638">
        <property type="entry name" value="Ran_BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00160">
        <property type="entry name" value="RanBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50196">
        <property type="entry name" value="RANBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RanBD1" evidence="1">
        <location>
            <begin position="30"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8169361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="168" mass="19586" checksum="550F058D2185CCC8" modified="2022-10-12" version="1">MDPHGDDDEQVGEDYNPEQEVFVGEGQATLPQLPVQTGEENDEEVAKFRAKIYRFADSQWKERGVGDMKFLKNKQTNKIRLLMRQDKTGKLIANHFITAQEGFCKLSQLKTADKSWIWTCYDASDEQPKVWQLCVRFTSPEEVERFKTEFEKAYESNKALQEEVPKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UXD7</accession>
    <name>A0A8S1UXD7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0490163</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UXD7"/>
    <dbReference type="OrthoDB" id="310362at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8168532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="16372" checksum="75374CB3167BAF93" modified="2022-10-12" version="1">MSQLSKSVSPITNTQTSFYSSSLQQSSYNSLTNSRQKERIFQQKQIPINIQKNSLFNLKPQILSNPNMHSDIRVSEFDKPIHFQTDQLIQRPVIMESKQKIQEQNINNFQPILEEKNDKFIKIGIVIIIVLLLLLGILKFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V7E1</accession>
    <name>A0A8S1V7E1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0590023</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000059">
        <property type="protein sequence ID" value="CAD8173260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V7E1"/>
    <dbReference type="OrthoDB" id="354826at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="23"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8173260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="510" mass="59215" checksum="997E6ACD557E1751" modified="2022-10-12" version="1">MNKLNELLIDQEERQSNFITQYCKPLRILGKGAFSTVVEIQNLMTNKKAALKIIEKQHFNTLQLDVLRKEAQLLNQLNHQNIVRVTFSKETKNKLFIMMDLITGVTLEQFEQNKLDDQIVHSIVKQILQAIKYLHSKDIIHRDIKPENIMISQMGDDIKVTLIDFGLSAQLTYLEGSGIMSDNCGTFLYMAPEMIQKKKYNRSVDIWAMGIVVFNLLTQGKHPFYQQFDDKESYCEKIQWMKWNWQPGMNNNSINFLIRTVAFLPENRLNINQCLEHPWITGKQDSSEPFTFIEILKSHCNFQKIKSLIQALQFLQQLKVLCPNANQFKHYSTPIAKEPIRTIKIPKIKQQSISHTNEKLKQVKQEAFQGSNVDTASIGSNRVSQFKIQTITLKSQRRERASCDQLQKLQQQKEPQKFLFPHFPRSLERSVKSIQTTNTTADNSLNQSSILQKSGIIDNRQSQNSKQTLQQLKRTTHVSRGQNIIIQQELLPSHRIRTNKPPIQKKSLNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V7H2</accession>
    <name>A0A8S1V7H2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0580003</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000058">
        <property type="protein sequence ID" value="CAD8172707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V7H2"/>
    <dbReference type="OrthoDB" id="434783at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051752">
        <property type="entry name" value="Mito_2-oxodicarb_carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46356">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46356:SF1">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="209"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8172707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="300" mass="35196" checksum="BB1D22A8C95DA731" modified="2022-10-12" version="1">MSESIYKVRTQKYFKPILETQIIGISRVILGLPIDHVFDRFKTLLQAENTQSSIKQLFKDTYQRNGVLKGVFAGFSSQISIQLFKQYYRWPMMILIPKYYKELLPNTWIENHPALHKGLAGTTIAFFESFVTCPLERIKCQLMTQHQSKSIFRQLWKNERSLSYFVRNLYTGMEAMVLKQVVSWTNYLYWDHKIRYFFKESPSQALTLPQIAMCSLLTAIPNILLVQPFDAVKTAYQMEKNGNYKQLTIPQAFVKVYKDRGFIGYYAGWQFRLSQFIIQAALSTPVMDYLERLHGLPNEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VFB6</accession>
    <name>A0A8S1VFB6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0620194</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8175143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VFB6"/>
    <dbReference type="OrthoDB" id="302776at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8175143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="189" mass="22183" checksum="105C41BBF3CE57FC" modified="2022-10-12" version="1">MQNKDSLFFQSQLFVAELCEPISLIEFVIDDLKPKQEIEQKISSGLQVSDRKQKINNQKERINPSISKVTHTQQSKNNKQDSISKGKNWFIKKLNEDNQSKQVLTCLPNLPKPIQNQGKLFSNTASRQIKQRLNKFMVDQNKNQFIIQNDILQGQNFSQYHLNRLKSDSPKQMPIYDVNFFQNFQQFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VGH8</accession>
    <name>A0A8S1VGH8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0640014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8175791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VGH8"/>
    <dbReference type="OrthoDB" id="300646at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8175791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="73" mass="8491" checksum="6F5F6320371062FA" modified="2022-10-12" version="1">MACCSCSQSLIFTIVLITLLFAAVTIDAVRFAWLTISFMFGIMLMIDYMFSYEKGFMYDPDYKTWRQKTQSDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VJR2</accession>
    <name>A0A8S1VJR2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0670237</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000067">
        <property type="protein sequence ID" value="CAD8177494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VJR2"/>
    <dbReference type="OrthoDB" id="10300701at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035865247" evidence="1">
        <location>
            <begin position="21"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8177494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="234" mass="26595" checksum="81040908D166F107" modified="2022-10-12" version="1">MKTLILVVLICYSSSQMLRGSSKSLVEEEQAIFQSEQEDQSFYVGRNDISAETLQGQETVEVETSLAVFKSDDNPMVYIEESIESNQDQNFEQQESLKIEKVDDEIQAQELPEATNDQANYVDKAIVLDPEQDQQASQNSQTDVVLNIAPKTESLNSSDANKKHIYVYNPLAKKPFVGIKFPPMPVTKDHNYLSNSESLTIKLPAWSEESNHRVEDDLEFFQFIRPSDVSVNEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VJS8</accession>
    <name>A0A8S1VJS8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0630209</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VJS8"/>
    <dbReference type="OrthoDB" id="309408at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="377"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8175702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="656" mass="76845" checksum="9C5647F7E4A6401C" modified="2022-10-12" version="1">MENELDESESKTFVTSFEEDVKILSKNYKGSFSFEVPKELEKFLDDNSYIDSIQQQLQTIINCPKLTQIKEALEKTKVENENSEIKQLQLLSKKEEDPQKTPSLKIQCNKHGKEIIMFNLNPEKTQFSRLACVECIQSNDPIKYTTLGDASFRWNEYLGQTSDQVKRFQNQRCLKSSQIIEILQEIKEKYNSIISEIINKVNTQYFSQNETNEFNENMIFEMSTEQVNELTEILSQNNKFQALAEKQLNIQKEDLNQLEIISTNFGKLLQNDLIATEKINKIFKESSFNISNEYKNEILQDDNPISNHLQIKQLNLQLQNFKIYQDILNEALNQYDLIIQTISSLSNEFKDIQLQQFKQYVSKFDKDLSLMQKFTQFDQIEAELKLHKQEKELLQNQLTESEKINLGNQQVIKDLKKNDAEQIKLISDLKRQNEGQSIKIKELEQQKIQCEVIVKEKQGELQSCQEQNQKNEEMIKQLQQTLKQEWQFSQSLTFSTTYKHSHCSVTQNGKVIETSQSGYLFSCMCDQMIPKNGVIQFAIKIIEITYIMIGIGFRDIVQSRNYLNCFEIGQGTYNIYYDGQCFNHDQQDKHRKQIAFPFSTNDIIIVEVDIQKKYVKWTKQSTNQSFTLNIDTSKDLYPCVQLQGKSKVEILNQVFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VL56</accession>
    <name>A0A8S1VL56_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0670119</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000067">
        <property type="protein sequence ID" value="CAD8177293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VL56"/>
    <dbReference type="OrthoDB" id="289935at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035804438" evidence="2">
        <location>
            <begin position="22"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="58"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="261"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8177293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="368" mass="41550" checksum="FBEA82F77418F888" modified="2022-10-12" version="1">MFKIAVCALLVLASTAINVQSSIWTSKDQKTFAQIHQSGWGKFILNFAELHLTTGGILSELNSEIEKLIGEMEEELAGVHHEFNRRTDVHNREVSRLEQEIQDKERELFNAHDFYDNVLIPQGERFAAQLEQLQENIAHNRQTLEQATVQRANDHETFEAQVVEHNDAIAAIDECLQLLSTLDTPSLAQVKKVQKNLAKIQNSLKKHNQFQIFVKVLLEITVDSNFADQGALRDIVVAFNNLRVELVDSLNQITADEADQVAEFNAQVIQLNQEHAEFQRAVVVKNAEIEANTTKQEQTLDLIDELDADLATLNGQLQAENDDYAFATDVYNATVAEYNKEINAANQALDLLNQPRFQDYVKSQLKGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VNP2</accession>
    <name>A0A8S1VNP2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0650046</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000065">
        <property type="protein sequence ID" value="CAD8176366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VNP2"/>
    <dbReference type="OrthoDB" id="10328920at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8176366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="153" mass="17434" checksum="F4A21536E469F454" modified="2022-10-12" version="1">MRQQQRIYFSLRLVEYFFLFISATIPQFKQLSRGISKGRKAFNNLKVLRISRTSIFGYIQSVYLLVDQICFIQYVSGYTIIPMDKLCKTLWTIVIILKPINQKSSSLIGIFLIGKFLDGLIGFSSSYIMLFEIPVAIAGLLGVGSSLIQLYLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VUD3</accession>
    <name>A0A8S1VUD3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0760151</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000076">
        <property type="protein sequence ID" value="CAD8181458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VUD3"/>
    <dbReference type="OrthoDB" id="303617at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="33"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="108"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="220"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8181458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="368" mass="43759" checksum="F3DF1F10C7CB0586" modified="2022-10-12" version="1">MSNKKPLSSEKKDNIIAQLKGELYQYHQRQIDFGSLHNQLSRLENAYSQVQEELQKIQKVGRAQTEVDLNEIKQLRSELEYLQEQLHQRLPNNNPQLLELAQIRQNELAILKKETNEIMLQKDQLQQERQELESTMKDMIETKNSLEKENQLLEKEFDKHFKQNKELEQTRQDLEFEISKKEKVILQQKKMVDMLNKEQRQKGDYLQQIQMKQEIQQQQSGQLDDQYQQLNENLEIVSKEQKLINNQLNQVQQEAKETNLENINLDKNIKELEGDIDAILNHVDEENLKKMNLVEDQKFLNVELNRALDLINQLTSIKQDLCEEIRTFISEDEQISNIVNRKNKQIKLIQYAEQQIEDSQSNNLNNII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VV51</accession>
    <name>A0A8S1VV51_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0770052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000077">
        <property type="protein sequence ID" value="CAD8181748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VV51"/>
    <dbReference type="OrthoDB" id="306573at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8181748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="235" mass="27726" checksum="538EC71524601BDD" modified="2022-10-12" version="1">MDQQAQETQNQYENIINEVIGKLKKNSDKGQLQMSESEIQTFKDLWQKCLKAIQDGNDTRFKQYVQPQIKQQIDNPKEYEPPIVKKNKTNVDTQIIKKEEEDDYNKADLAFSDDSGDETSSKEQKVDNQKKYLDYQESLREKTLFDKIRELEQQKNLIKVEEEDENESEEPRIFDTQVYAQKINTENAVNRIRPNKSQKMSLENVLIKEKNNKEALYPQAQLQFNFKTSKKKTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VVW9</accession>
    <name>A0A8S1VVW9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0750135</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000075">
        <property type="protein sequence ID" value="CAD8180971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VVW9"/>
    <dbReference type="OrthoDB" id="8063680at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04369">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45926">
        <property type="entry name" value="OSJNBA0053K19.4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bromo" evidence="2">
        <location>
            <begin position="14"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8180971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="195" mass="23231" checksum="F2B8FF2F24ADE20A" modified="2022-10-12" version="1">MMAQLQQKLLEIIENLINNPISLEFIDPYDDEDYNKVILNKMDLTTIKKKLESNKYINHNEFFKDLDLVWKNCQTYNQKGSIISKQSIYLEQQCKSLQKVIEDDFNLGKKKKRQNQERLPIQQQSFDFRQKNKPSNEASRLKFCHQIKNLEQEQLIKIVNYLAEFQPECLSREEDKLVIDVKKLDSKACEQINLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W0V8</accession>
    <name>A0A8S1W0V8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0820073</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000082">
        <property type="protein sequence ID" value="CAD8183968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W0V8"/>
    <dbReference type="OrthoDB" id="310085at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8183968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="106" mass="12157" checksum="D304B9EF303B29A2" modified="2022-10-12" version="1">MKSVQNFQIVQNMKAYFSDDIQSLQKRIDGPITSYDSISSDQMLLPDLKSIILNQNDLIRENYQGLLPTETYSRSLTTNSSSQLFRFEEQFISVNSISSESVSDEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W5P6</accession>
    <name>A0A8S1W5P6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0830159</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000083">
        <property type="protein sequence ID" value="CAD8184630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W5P6"/>
    <dbReference type="OrthoDB" id="296522at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47823:SF9">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_10, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47823">
        <property type="entry name" value="ION_TRANS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="291"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="330"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="375"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="405"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="537"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8184630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="928" mass="111267" checksum="69CA5EFC93C58904" modified="2022-10-12" version="1">MNNKCELIIQESGLEMERSLDHSIIRSPQAEQLISSSNKQTLLKFDSSFAQESFQKRKDSLSSQNLSDKNQMITTIIRQKQFSKLLIYCHSMKFANRLISLIKPCSKFSIEQFNMINDKASFFNNNLQTKKQTTKHFVQQNLRDIDFQFRWRLQLRKFKKRLVEYLHYQYQQIPLIEPESQKLFIWDILLNLIRIYLIIWIPIIMVFQNQQLEYNNRIMILFSSILLLIDLFLRCITIVYDKGYPVDNRYLLIKRQIKLSNLFEVCSIFIGIYLSIRFSDPEFNYSILDENGWIKLLLILFLIQIKNVVKFMNNIQYYYKPNKEMSSVIDLIKLVGLILLIQHLCSCVWLIIGMNQLTQHHQSWIYKVFLESWEVQYLEAFYFMSVTMFTVGYGDINPQNSLEKVFCICYMFLSTLQLSYSVNTIGAILTLLKEKNEIFRQKMTCMNEFLKDKNISQQLQYKIREFCTFYWEQDIIQKKNEQQLLIKELPDELQYHLKLEQAKQLVNKCTFFKQHFAKSTIQYIIQRVQFTSYQPGTIIQITPSNNGVFVIERGNIEVLQNKTKISDLKDFDQFGFQEYINNQYNLDIIYQTTTYTSILCIPYQILNDAIKKSKSDFEKIQIKKQITKFHFCYICKSTKHSIEQCKLVHYVADKEKSIKTYLLVDNQPRIKIKRSNRFQKFQTLIQQVCLDASCKLIQQEYEREIEQYFPELTYEALIKPLNSSQKIFESPKDNEVYRPQKQNYLLETIKTSQVRTYDKFCNNLSLTFRNQDISPFIKEPDILKELQLKMINIQKYPQKQQKELFILYKTLENQSQADIGIGNFEKVIKFTKFDRFWNISNVIEQLNCKYEHSRLYLDIIKRLSKYLLFPLEFIYLFKKDLLKQRAESYQNRKIKDSDDEKSDNKQISPFRKFERRKALIYPKSLTFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WE31</accession>
    <name>A0A8S1WE31_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0890074</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000089">
        <property type="protein sequence ID" value="CAD8187433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WE31"/>
    <dbReference type="OrthoDB" id="289533at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8187433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="218" mass="25788" checksum="78C9C5F30CCDFF78" modified="2022-10-12" version="1">MPPFTPDARNLGGRNMISQQIWNHLQQLKEISQQIPQILISPRKLPNSPKQSIEAINYKRVNRKQDYLLYPTYLFRRKTVSESFTSLSPKNIRDETQLLFQEPLLRRQKNLKSPQKIFPFSTIQLGALHKIKANEIKMNKKNDEILWSEVEMTVKEFNTKYEDLLNEIQQVIQEFGIYSASDYQILIHIILRLKPQIPKMDLLNIFNELIIKQQLQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WER4</accession>
    <name>A0A8S1WER4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Actin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0830056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000083">
        <property type="protein sequence ID" value="CAD8184426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WER4"/>
    <dbReference type="OrthoDB" id="289571at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8184426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="214" mass="24723" checksum="319D3D474BBB868D" modified="2022-10-12" version="1">MHALEYSHISGAEITQCLENDLQTYEISLDKFSEEEKQWIIKDIKEKMCYIANDYEMQINSEDNFTEEEKSYELPDEQVIHITPKIRYHCSEALFNSAIINKNSSSLPQMIVNSIQRCDKELRQELLSNMVLGGGTSMFQGLISRLQDDICQIYPGGAMRSEFNFVADFQRKYSAWIGGSMLGSLKTFQSLAINKQEYEENPEGKMSLIHKRTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WF09</accession>
    <name>A0A8S1WF09_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0900086</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8187873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WF09"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18579">
        <property type="entry name" value="ABC_6TM_ABCC_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18580">
        <property type="entry name" value="ABC_6TM_ABCC_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000080">
        <property type="entry name" value="ABC transporter C family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000063">
        <property type="entry name" value="Multidrug resistance protein ABC transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000610">
        <property type="entry name" value="Multidrug resistance-associated ABC transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000997">
        <property type="entry name" value="Multidrug resistance-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044746">
        <property type="entry name" value="ABCC_6TM_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044726">
        <property type="entry name" value="ABCC_6TM_D2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF456">
        <property type="entry name" value="MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN LETHAL(2)03659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="292"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="329"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="679"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="715"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="804"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="63"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="384"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="680"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="993"/>
            <end position="1226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8187873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1234" mass="140550" checksum="FD96AE8112AA823D" modified="2022-10-12" version="1">MKVRIAQQHQLTEKEIYEYEEQTPSEALFWQFYQLKSNQYSLMKQLILFFRSKGFQIFLGRLIMIFLIQIVQLSAQLVMPFVIRQVLTYVQKEDKDLLDGLILIGIILMVKVISLLSANHLKLRMRLVGYDAMSILSMQIMNKCLRLSMLSNTQRTIGEITNLMQVDAQKIITAANNIMSIIIMPIQTSIEQVQFFLVITLTFIYQQIGISVLVGIAIIILTLVINNYLGKNLLQSQKQVLLSKDDRIKQTNEVFQQIKFIKINSYESIFQSKIEQLRELERKCIDKRLQYYSLNVFFGWLSPQIILSLSFGLYIYLGNQLTPAKVFPIISLLLMLAANLQLFPISINALLEISLSLKRLSVFFETHEIMDDCISKNTDKDYSIQIQNGNFSWNKDQLQNVKILNNVQLNIKKGAFISIIGDVGSGKSSLIQGLLGEMVYDESKENPKILICGNLAYVGQKAWIQNGTVRDNITFGKKFNQDLYDQVIYYSCLSQDLEILIDGDLTMIGEKGINLSGGQKARISLARAIYSGAQIILLDDPLSALDVHVGNFIMKECFLKHLSSKTRVLSTNALNFCQYTDYIYLIQNGKIIDEGNFGKISQSTKFQEIEQSNVLKTNQKTDVQQNQTQTTQSKIQSIQQNKNKAKTEDIILKEDRQIGKVDYEVYQKYFIYNGGLKNYVILILIMILWIISQLISNFWIAKWASDSNSHDHNSYVYLSVYFLLGIAQSLFAYARAASVVNSSLKSSSRIHNEIIQSLLKAPQCEFFERIPIGRIMNRLTKDINSLDIDININISLFSTKLSQIISATLLAIITSTKLIIAPFILFFYLSLKIKNIYMQASRELQRLELITKSPILSYFVESLQGLTIIRAYQKSNLFLTTFSQKLDQNRQISYVSTVANCWFTQVLGFSSLIVNMTAITYCIVIQNNASFIGLILTYVANLDQLIQQTIDTLSTLENNMISFERCLEFTKIPQEINTPTLKVEPDWPKQGLISFNNLSVKYRPDLPLALKNFSYKINNNEKIGIVGRTGAGKSTLALSLLRLLEAQEGQILIDNIDISQISLQILRNSITSIQQDSVIFNGSIRQNLDPFQQHDDDSIRQVLNDCCLTNLINQRNGLNTRINESGDNLSAGEKQLMCIARAILKRAKLVLIDEATANIDFETEQKIQKVISDQFSNCTVLIIAHRINTIMLCDKILVIDNGILVEEGSTQDLVNNPSSIFYNIYQEVIKNEVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WGR9</accession>
    <name>A0A8S1WGR9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0900093</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8187885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WGR9"/>
    <dbReference type="OrthoDB" id="289272at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="240"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="10">
        <location>
            <begin position="499"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="75"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="592"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="607"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8187885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="713" mass="82185" checksum="B915CDA9C59EC7B4" modified="2022-10-12" version="1">MLSKQQQQLQEIVSQSVLKTYTTKASQKLSQSFNPNGLIGQSLSPTQKTNQSMGLKKGNNLKQYQQELLKLMTSHKQLQSGTKKANLSDMMTQQKESKRISSGTSQPQKSQNSNKISFVNPANQIIIIVNHENQQIKFTIDPQKSTGWLEEYLKQEVKKHSIHQYSSTGSQPDERKVTCGTGNESSDLEIVSFHTVDKNLPIDYYLQQANKSLEIFSGQTLHLQPFYGTPQQSKITLKDFIFVKCIGVGGFSRVYMVKKKSNGRFYAMKLIDKEFIMQHKKQGIVQNERDIMTVLDHPFIIKLEYAFESKNFIVFVLEFCSGGELFWQLRQVKRMSEDQARFYFAEICLAMFYLHSLSVVYRDIKPENILIDIDGHIRIADFGLSKPNMTEEDYAYSFCGSPEYMAPEMLLKVGHNVQVDHYCMGALLFELVTGLPPYYSRDTDEIYESILNEELSFPEKLNLSPEIKDLLQGLLCKQPSERLGANKGLTELLTHAWFKDVDLVAILQKQIPPPFKPNQLKFNYDSNDLMKGELETREKLLGKAGLQQEIRIFKAFYFDSYEQKQMKTEQAKILQQHFMMVTQQQLALNQKFKPQRKSTEPKEYQSKPASPQTRNQKQQKFTPEQFQSLQKRIKSQQSSMSNVQQISTIQSPAQSKLQGLKRIISQNNFFADRQNTLPAGQKDVNIDYQQLLSNVSTDQMLHKRVSSLKQRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WI58</accession>
    <name>A0A8S1WI58_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T0910113</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000091">
        <property type="protein sequence ID" value="CAD8188385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WI58"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF32">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="130"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8188385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="566" mass="65150" checksum="58401BAD876E4129" modified="2022-10-12" version="1">MMLNEFTSFFDNLEVINKIKHIWSKFQLSIYEKDSIIFESVCHKISRKTQKLKPIIIQLGQEHLYLFKNKNPHGMLLLTVVVMTIQKNENGLMIRLLKNGQYIDIITTDAMLLKQLLAFKCLQTTFHEEFGVTKMIGKGSFAKVYLASKKQTGAQYAIKAFNKEFMLEQFKGKESLENEIRVMRRLNQDNLVHLHEAYETQNSIYFVIDLLQGGELLARAKTNPFSLDTLQKLMYNFIKALVHIHSKKCIHRDLKPENLLLKSKDSNVDVVIADFGLAAFLGEEILFKRCGTPGFVAPEILMYKEDDPFYDEKCDIFSAGVIFYILLTGKQPFQGADYKAILRANKNCEINYNIKQIQQASPKLQELIRKMLQQNPKDRPSAEACLQHPYFEEIFNKNDLVEIHENLIEYEIEHEHRLQKKGSFDSQVGSMELVVRTPILNGRTDTIGSLSVCSGQGSTSRLEKPQPQLQQQSKFSQFCQTMKTVQQDTNTNTMASPQNKKKDQQDLHKFALKNSYQQKQMSKDDDFVNEESAHLDDAIQKLNSQAPKIGLFKKSASYKVPKSTQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WJY4</accession>
    <name>A0A8S1WJY4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0910146</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000091">
        <property type="protein sequence ID" value="CAD8188449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WJY4"/>
    <dbReference type="OrthoDB" id="300207at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035784989" evidence="2">
        <location>
            <begin position="18"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="295"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8188449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="347" mass="38698" checksum="08D07EB86CB34C66" modified="2022-10-12" version="1">MKGIIAIILALAVVSNATSTKDQILALLQTGTKASDAIDTVFGLLNDLKQSNIDAQFAADQKNETDEWVGAQTIEQFTKIKTLNQKLFQQAIENRAEYENVLQQTKNYLAWNEARRDSIAAKIETLQDNQCFSNQLFVKSIKHNQEALEVIRLLKQDVAGYIINGDSFEFTQVKAQSVAEKLKQYSNLFQEHQIKSFLALAQEEQASESSGHGQTLAEKVLGVLEGLEAELAASLENLKQNEINASWELAGWVSLSEAEIASLEVEYERKQVFADRTATQIQAALAQQAKSKIILQESQDALDQAQSDLEAKRADYAEAKAKRQEENAILDEVIIMFKKQVASWSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WMW7</accession>
    <name>A0A8S1WMW7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0950067</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000095">
        <property type="protein sequence ID" value="CAD8189971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WMW7"/>
    <dbReference type="OrthoDB" id="300179at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8189971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="104" mass="12387" checksum="ACE3B58059793602" modified="2022-10-12" version="1">MIGPKQLLLIPLKYYQNHEDQNVQCLDDFELKIQSNEEFSTTHIIQTQYSHKKVQKIDKKVKFNPTIISYKFRESEPAITISKQMKKLIQMQPNLKWINPNIQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WN39</accession>
    <name>A0A8S1WN39_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T0880170</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8187146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WN39"/>
    <dbReference type="OrthoDB" id="444079at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="287"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="329"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="361"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="8">
        <location>
            <begin position="465"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="9">
        <location>
            <begin position="596"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8187146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="930" mass="109486" checksum="DFC75C9759236424" modified="2022-10-12" version="1">MEIDQYSARAANEDQALMSKRGDQEQRKSEFFQRCLQDRKVNTDLSESEKFIFGDKTIYMGRMKLEQRLQDIDEVFLPFDNIDLDEKERNEKMKELSKSSQSDNQRIDINKRNKKIENFWLIISPDSSIKIVWDFFCMILILYEIITIPIRISFDIEVSAEFGYVITAAFLFDIVLTFNTAVYINGNINYSYKAIAFDYFKLWFWIDVVASFPYDMVFNTALTGEAGDEVNDSSENLKKSTQILRVLKFFRFVKVIRLLRLAKLKAIMDKIEEYFSDSSIIQTIGSFLKLCAFVLFWSHWLGCIFHFIGQSEDTSYNWLSIYGLYDEPWEIRYVNSVYWAVTTMITVGYGDLSPQTPLERLFGVFFLLIACGVFSFTMNTIGNTMQQLSQKQDQYQKRISEINIYMAKVKIQKQLQNKVRRYLQYIWDSHRSVNLESICQNLSLSLKYEFTIQVNGTILASYKLLCETFSRKLLIELTQILKEQTIQPDEYVFIEDEPKNEQILYFIQEGQINIVLIKTRQIVARLSNKQIFGEISFFGNIGRTASAKSNGFTDVFVLRRQDFVALLDKFPDDRERFNFINEEVNKRQLQILDIHCYACDLPGHVIRECPSLHFVIDLDVYQKTKIRCIKAIMKDFVRKDRMNYNAMKNRTELEKAAKSLQMNIPTHKFLMEECNVDEQLSQAVEDESIKKGFKLKSKFKEDRNRRRNWEQSYKKSAQKIQQIQIKQSEIFSITKSLSQAILSSNASGQTSLNHIAINQFKSPNESSEESDSGTIQQKMEDIMADYQIFKQEVDKYNGSQQLEIKKNFVLLNEFEFGCDYNIFYPHNNLSVVLKEFHKYQLGNSVKPKIDTEYDTNVFQEYMEYYVIDIEDVKRFKLEVKPSQIKQFLPFTELLQSSYNRQKKKNFGLLKQPKRSFKQVVRAIILARKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WNN2</accession>
    <name>A0A8S1WNN2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Polycystin cation channel PKD1/PKD2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0990127</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000099">
        <property type="protein sequence ID" value="CAD8191614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WNN2"/>
    <dbReference type="OrthoDB" id="293784at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072345">
        <property type="term" value="F:NAADP-sensitive calcium-release channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000489">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039031">
        <property type="entry name" value="Mucolipin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013122">
        <property type="entry name" value="PKD1_2_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12127">
        <property type="entry name" value="MUCOLIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12127:SF7">
        <property type="entry name" value="SD02261P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08016">
        <property type="entry name" value="PKD_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="233"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="347"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="384"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="451"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Polycystin cation channel PKD1/PKD2" evidence="6">
        <location>
            <begin position="338"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8191614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="657" mass="76339" checksum="EADA5BE909DD9170" modified="2022-10-12" version="1">MSEGIYTETLKDKFTLSPFEKYHKYGRFPFKFLISCALVFLTTLQVLLISEHYTYNNSGQIIQFVALLLNADEPMNNKITLLTIDDFQDSLNKTLNNLANIDDITVQNTTVQGNTFFQINYNYPFEAYRIDYPFVRNVTLGEPLLDPFDLEDDDQIREFWTNAYELTLTVNQIKVESQQRSACWDIQITYQFINYGYIDAIITSIGGICKENREEEEGYGSAFNSTALRIDNLLLQLSIILLAFIDVCLGVKYIYEVIEMYTNNISKLKKQRKLKVPEYKLYKNQNLTTLKIILNEQKPWEQLTIKEKLLFFDLFLPIRILGNIIQLISAGILISETFIRVDGLSQYDELMAGLGCFFAWFSIVKYLDYYEELHLIASVLESSIIPVCMAILNFLPVFLGYALLGMCLFSDSHQFVSLSSSINALFSLIYGDSISDVARDSSRGVSLELSLIYIVSYILLFLFAVHNIMVALIKEQLEIRKDLIFQEKELMNQLDLSNNYQYQMYLSQKMKQTTIMNRSVMKDDFGSMMQSQTGNNQQQQQINKQISLKSITKLAMLQKQKSVDISKQEMENNWKQICKEEMQNHYIYNILISTITETKNILSNVELELWNDIDSSPFTVIDKKEIITLYCSQLIKKHQKMRNIFNKTKQISQNLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WP68</accession>
    <name>A0A8S1WP68_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Armadillo-type fold</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0950106</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000095">
        <property type="protein sequence ID" value="CAD8190049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WP68"/>
    <dbReference type="OrthoDB" id="311327at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052623">
        <property type="entry name" value="DAAF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056497">
        <property type="entry name" value="HEAT_DAAF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057978">
        <property type="entry name" value="TPR_DAAF5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16216">
        <property type="entry name" value="DYNEIN ASSEMBLY FACTOR 5, AXONEMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16216:SF2">
        <property type="entry name" value="DYNEIN AXONEMAL ASSEMBLY FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24573">
        <property type="entry name" value="HEAT_DAAF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25757">
        <property type="entry name" value="TPR_DNAAF5"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein axonemal assembly factor 5 TPR repeats" evidence="2">
        <location>
            <begin position="23"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein axonemal assembly factor 5 TPR repeats" evidence="2">
        <location>
            <begin position="137"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein axonemal assembly factor 5 TPR repeats" evidence="2">
        <location>
            <begin position="276"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein axonemal assembly factor 5 HEAT-repeat" evidence="1">
        <location>
            <begin position="377"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8190049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="926" mass="107953" checksum="1A0F7EC59B62885A" modified="2022-10-12" version="1">MNDFIIGDNLELESFFKQNTNALHEQNPTLRKTAITQIYQKVTDPNIAQSDSLSQQILLKYGKVLLKSFSDTNEKVREMSIKSMIQLITNSEDNEPILSYVFSVLIERSNCQDLEGISHLPEVMRPPPSQLPTRLMKFVEESEEIRLLLTQFLYSIMCKNDSDNLHEYIDDIVDIHRAFLMDPFGEVQKEACKSLGKFCKVNHQMLLHFTEKMGRAILHPLVNKKSPVRIAALEALSAVLLCGVWKNNAFVFEILTGFKDPNSVPIKAFFEPMHHINYLATLIIDPKQQVRQAFIKEIGFWLRGLADRWDHHPRLVPYLLSGLFDTDPSIQEAALQIIEDVGKEWEIEKEKDIKNVRQLGIEPGWTLDGKLSQLPLPPPFKERPKLGMRGFIKSQVRKMWPAIYKEIIDNNEENRLRASHLMTLGVLFSEDYMTQFLDNFIPNTIMAIKSGQTKIDKNVQLNLQKTYQLLGRFCDYTSYSFIIFSSMRGKYIRDEQYIRSATQALSWLCQGAIEATYQGIGQKMELLNNIVDLLCENSMIESISTINYLEIQELLSTVKKSIEKMGNQDEVIAFNQKYQYQLNLLDNVINYILNFNGVLLNQQIQQSQKLQYSNDRIKLLKVDNMKNDFLFGDSGWKNVISELIHQLHYGSTQAYQLTDILYDFILPNSLPEPCDFILKLTQLLLQSFEQQHSQLYLQIILKLCAIAGQWLNSLQQKQFTQKLLDNMLVTFKTISGYLSKKRDIGKIMCKDQLSLDYYTRIVISLCNSYQKFYRKQYYMIVVCISEFCNLFLPYPSSKHLLDSKNQLYLLPNLQNFCFDAIDQYGGEQNQETRKHLFSILSYINQFIPVQETYLGDPHVIENYQKYIQQLLRLCIEEKDLQFQDIYKIMIKELCNKTRNNFLQELEISKHNNQLSRVELLYVIINQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WPF7</accession>
    <name>A0A8S1WPF7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA (guanine(9)-N(1))-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.221</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0950141</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>guanosine(9) in tRNA + S-adenosyl-L-methionine = N(1)-methylguanosine(9) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43156"/>
            <dbReference type="Rhea" id="RHEA-COMP:10367"/>
            <dbReference type="Rhea" id="RHEA-COMP:10368"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.221"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.221" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000095">
        <property type="protein sequence ID" value="CAD8190119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WPF7"/>
    <dbReference type="OrthoDB" id="278300at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052905">
        <property type="term" value="F:tRNA (guanosine(9)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18089">
        <property type="entry name" value="SPOUT_Trm10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028564">
        <property type="entry name" value="MT_TRM10-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007356">
        <property type="entry name" value="tRNA_m1G_MeTrfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13563">
        <property type="entry name" value="TRNA (GUANINE-9-) METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13563:SF13">
        <property type="entry name" value="TRNA METHYLTRANSFERASE 10 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51675">
        <property type="entry name" value="SAM_MT_TRM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM-dependent MTase TRM10-type" evidence="3">
        <location>
            <begin position="67"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00048434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8190119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="272" mass="31912" checksum="72160F28E9A0C0B2" modified="2022-10-12" version="1">MQEQQIQEGLKEKKPKLTRDEKKVLKYQRAKLRAKILRKKIAKKVKQKVHHMNQEAKEKYSEIKNLAKNNLQLGYESGIPIYIDLEYDVLMTDREIRSLALQIAHVQGINKELQFPIQLHLTNVRGVQKNELEKQGLSKWPVHHYELGLQELIQESLEKGISKEFIYLSPDADTYLESVDPEKCIYIVGGFVDRQVQKYMSLNKANTLQICSKKLPIESFIKGTRRPLNIDRVVAILSLFYQHKDWKVAYEQSDNFNISLAERISRQHQKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WQ44</accession>
    <name>A0A8S1WQ44_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD domain, G-beta repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0970173</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000097">
        <property type="protein sequence ID" value="CAD8190900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WQ44"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="194"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="239"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8190900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="448" mass="52311" checksum="FEFEFBB0BAB944AD" modified="2022-10-12" version="1">MQIRCTQADHLNQLIIGVCIDSDCQYKRPNCNFCLPHHGQHLNKLTSLELFDDWIRKRALIVQNVEQKAQECKLALDGLLNKFINPYNINLAQFPKLELSQIDNIIKGLCLIELWENLFQQSIEEIKYIFKEILQTQKYQTFTFELMNQNSIKQQDVCFAIAINKDNSIVVAGCDKQIKVFELKESKLNQLQLLSEHQLHVQALNFMKKSNHFVSGSNDKQIIIWQMNQNNQWTCQHKLDGHSFGICTLILNNNENLIVSGSYDSSIKFWNKQNGWLCQQTIKVHTNYVFQLSLNEKQDKLVSCSSDSQILVIEYSQKDQIWTVIQIIQVEQYGYRLSFVDDNQFLFQPSSKNKCMYMRQTQITVKSGSNTCGDLFPQQYIQSKCLLVNKNGQNINLVRKKLNGEFETEQTIQFDHHYIFGQLSNGGEYLITWDFTSKEIQIRKCKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WSS9</accession>
    <name>A0A8S1WSS9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cilia- and flagella-associated protein 58 central coiled coil domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1030108</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000103">
        <property type="protein sequence ID" value="CAD8193104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WSS9"/>
    <dbReference type="OrthoDB" id="264785at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049270">
        <property type="entry name" value="CFAP58_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32083:SF0">
        <property type="entry name" value="CILIA AND FLAGELLA-ASSOCIATED PROTEIN 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32083">
        <property type="entry name" value="CILIA AND FLAGELLA-ASSOCIATED PROTEIN 58-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21771">
        <property type="entry name" value="CFAP58_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cilia- and flagella-associated protein 58 central coiled coil" evidence="3">
        <location>
            <begin position="367"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="90"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="468"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="650"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="742"/>
            <end position="834"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8193104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="865" mass="103395" checksum="3B6EB371B1F1B183" modified="2022-10-12" version="1">MQNQVDLDIDTGQFDTMERDFQEVLRDLGTDQNLERFRSEFDKLYRSLKIIHENQRRLISKCREYNAEISQNASKIQTVLKMTADDSAAIQQLKTQLEKAYKVLEIQQEREEKHKQKIKIQENEIKQLNQTIEQSKALNSGQTTTVHELLQRKQELLKEKEILQIQVFGTRSENQSITDRIKSLETSKESVMKEYRILQQQKSEFEERLQKDEEAKNVTQAELEKIKKDFDSLKDDEKQLGNEKKKLKSEIEKVTLQNSVKTKEIENFKQEKNRVDKELRDTKEKLDSVEKINEQIEEKIKDTRLQIELFETEIAQLHQKINEGEEKKKRMTELVKEQEELEEKIEEEKLLANNGLNQLEDDIQIERKKASDDRQVIEDLRRARNILQKEIDRCDNNNKKIEEDFIAKQKYLSEKQNELGGLQKKIDYLNKKIANVEKETEQQCLQFSQAQTKYFHSLDEIKLKDSLISEFQKKNIETEAKLKQQQNLYETVRSDRNLYSKNYTEKQQEIEKMRRSYKIVNHQISQLKEEIEAKGNALAKEHLEHKKKDKTIEEQSRVLEKYKTDIDEKAEKINKYIKRVDKLQFTIKDEEQQIQNLKEEFELVVAERDILSTQLIRRISETNLLYEKIKINESTLKKGEQQYRERLGDIQMLKEKIADYKREIKVFKREADTIKDLEGDIHNLTKELTEEKLKAKALTEELENPMNVHRWRKLEATDSENYELMTKIHSLQKRLIQKTEEVVIKEKVVEEKEKELKALKDEMKRKPGLEDQAMIPYYQDSLRQKEEQMKAMDQELSMYQSHINEYKLEIDRINKELQRVKQKYFNQKKREQQQRDLRIQEEQGQSIQVILPEKKFVGGGFALQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WWG4</accession>
    <name>A0A8S1WWG4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Signal recognition particle SRP54 subunit M-domain domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1050040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000105">
        <property type="protein sequence ID" value="CAD8193782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WWG4"/>
    <dbReference type="OrthoDB" id="300731at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048500">
        <property type="term" value="C:signal recognition particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8193782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="202" mass="24417" checksum="364F31BA2FF17C28" modified="2022-10-12" version="1">MIKLFAQKFIQLQIQPLYEFNRFADKLQKKQQDNQKQEFKKELEFLASKPQFTLIDFRQRALDGLHKLKKGFKYQISSGNEETEQQLTLQRQVLNAMFDEELLNPDDLTGDQKKEIAMVAQVEVQQVNFVLSQYGQIRNMHSWVRDMKERGEPMPDNQEDMMYRYKRDKPIKKSFIKFEMKRQNFSMKQRLQKLKWGPRKEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X2G2</accession>
    <name>A0A8S1X2G2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1070184</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000107">
        <property type="protein sequence ID" value="CAD8194830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X2G2"/>
    <dbReference type="OrthoDB" id="308440at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="208"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8194830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="307" mass="36603" checksum="6F4E39F4C3B13E7C" modified="2022-10-12" version="1">MKCLLLNHNSQVERYFINLKCIEKTSLSCNECYLSSFYCNHQKDYNLISDLHDYVEKISTQCERELIKFLGDLEDQISGSFSHQKIKLDHNFKYPHSVNEMLCQFMDLNSFKDDMIEIEIKSKIYQMNQMKKSNSFVLNKQYKSLDKKLLKQTHIRLKLQKHLILLQTLIHIKLKLCCGRVIRKIISSSYRDALQQFKQVFKLEESNIKSLYGIGDCLFRLNQYQEAIHWLDQALKYNPKNATSLSKKGDCLKNSKNYYEAIILYDNALSIDSNLFWTSQSKCFYQYQNYRIMIIRFKAIQRSYRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X634</accession>
    <name>A0A8S1X634_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1050182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000105">
        <property type="protein sequence ID" value="CAD8194026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X634"/>
    <dbReference type="OrthoDB" id="10001977at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF122">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN SKYWALKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="1">
        <location>
            <begin position="56"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8194026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="230" mass="27184" checksum="C8499051B8DC27CD" modified="2022-10-12" version="1">MNTVVQENTKDQKNHEDLLKHLVIQSKNDLLNLEKKNCFLQQIFQKNKFDQILKPNLLKADYWIKIFYILQERTKKNIKQPVLIYQGTRDGLNNQQYWKKVNGKENLLMVFKSKSDYIFGAYSPCKWESCNGKYIEDNTLSSFIFSQTHDQVYPLKQDQKQYAIYCYSGNGPRFGQGYDIYIQGDFNDGNSRLGCSYQFNQYKGGNDDPYLFGQNKPEIKECEIYELQFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X682</accession>
    <name>A0A8S1X682_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Polyadenylate-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1060032</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X682"/>
    <dbReference type="OrthoDB" id="296989at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001457">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001458">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1900.10:FF:000012">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001005">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002004">
        <property type="entry name" value="PABP_HYD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00658">
        <property type="entry name" value="MLLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00517">
        <property type="entry name" value="PolyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51309">
        <property type="entry name" value="PABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="21"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="107"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="349"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="PABC" evidence="4">
        <location>
            <begin position="542"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8194116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="625" mass="72742" checksum="EDA515E5B2528A1B" modified="2022-10-12" version="1">MQPKEIHQQIPPQTTPQQMKTIIFIENLDQGISEDYLYRKFKEVGEIQSLKITKDKTTQKSKGQAFITFAHPDSAEEARKKFNNQVFIRNIIRVKQYFNYHNADKKANIFINNLSEDADILELEQEFSRFGTVLSVDIHRDSQGKQLNYGYVQFEKKEDADNLIKRIMNHPMTHKGKLLKLESFKAQSERKVESSSIYLRAFAKPLQQNYFEKEKVVRAIEHGWSLIIKDYFTKHGFQIKDCFVKIDQHTRQPWTMISFETSEQAKYHLDICELSRTHPCINSGAHHALELINKHGPPVNSDASQAFSVAEITQAFKEMAQDHNDTFKGESENFFYNMVYSKNLNPDDRLILVQNIKDDVTKEQILDFLSRFGKVIRLTIRKTKNPRFQVQQCFVHYQTMEDSKRARSEIYDDKNEEISKIKKQIFKDGHVIMNILLSKAMRKEFKEIKKQSQSIFQNPGGRQLLQPPFAGNLQMMPPQMPFPPGQNGMRQIPPRGGRGGPFTMVRPQGIRPPPMNNKPFQPPPNKVVTSQTFIQEFSDYTLIQNRMEEFLKIPQSDQRNVLGNLLFQKVFEVVKDKESTKKVVGMLIDPSQFEIGDILNMFEDSQELQTYIDDGLALIKEETQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X785</accession>
    <name>A0A8S1X785_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1130094</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000113">
        <property type="protein sequence ID" value="CAD8196689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X785"/>
    <dbReference type="OrthoDB" id="298330at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8196689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="321" mass="38157" checksum="896D5F8AA0B5579F" modified="2022-10-12" version="1">MRQKHLVNELINIRDEMKHINNRIKTNSSTKNHEFISHQNTNFKIRKYCHQLIQIKPPQFFKYFISEYFLLDLLEKSDYEKILKITLKFIVKHFQDQELYQKYDNQYQSNEKNAEKINKIIKEVVSPIKSKIESQTQFYDQQLKINHRSCQTQPCYSESQQVIQFNLGLRQQSSIISSIQQDNLNSPKFNENTINAIDEISFEKSCIKKGSENQIGNHTKQNQLENNEKKHPYSVSLLSTPKQNEFDNKKQGIFNNNNITVRETKRKEKHKTSLEFLTYDKQDEINKIKKQLPSTMLSLDFIKQRKKIPESKKGSNILSNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X8W2</accession>
    <name>A0A8S1X8W2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T1150102</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000115">
        <property type="protein sequence ID" value="CAD8197430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X8W2"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001624">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="55"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="83"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="133"/>
            <end position="135"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="182"/>
            <end position="183"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="8">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8197430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="397" mass="46326" checksum="54B737A42A37AB0F" modified="2022-10-12" version="1">MNKIVIKKKEDMNQKVFQQLNDQVPTQYKNQLLCQIDPQGSQRDNNYQKSEKDFQIIEQCLGSGAYGSVQLVRDVNSRIYYARKAISKSKLKAQESIENLKREISIQKKLCHPNILKLCYCYEDQLSVLLILEYAELGSLFSLIKRKSRLQEREAYQYFSQLLSGLEYMHKMNIVHRDIKPENLLITKSGELKIGDFGWATQMPNYHKAFCGTIEYMSPEMIQSQITDYKTDIWSLGVLLYEMVQGKTPFQGMTFLEKSQKILSRKEVEFEIDVSDECKSLIKSLLQHSIHCRPSIDKIKNHQWILSQGQDRKGSIRSSSMISIKSTHYTSEVLESQSKIYNPQFGQKSSIQEWEAMKFRSLQFQFQRQQDQQQPQNNYQNKGFLTKALIALGCINR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X8X3</accession>
    <name>A0A8S1X8X3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cysteine-rich PDZ-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Cysteine-rich interactor of PDZ three</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1150105</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRIPT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000115">
        <property type="protein sequence ID" value="CAD8197436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X8X3"/>
    <dbReference type="OrthoDB" id="147332at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019367">
        <property type="entry name" value="PDZ-binding_CRIPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11805">
        <property type="entry name" value="CYSTEINE-RICH PDZ-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11805:SF1">
        <property type="entry name" value="CYSTEINE-RICH PDZ-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10235">
        <property type="entry name" value="Cript"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0747">Spliceosome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8197436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="91" mass="10630" checksum="4F9DC113CB65018A" modified="2022-10-12" version="1">MVCDKCQEKLTKLATPDVWDKDNKNKKPGMILPSFNKNKFDPMGKNKCQKCKKRQVQKNEKFCQECAYKDGICKMCGVKVLETKFYRQSNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X902</accession>
    <name>A0A8S1X902_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1150120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000115">
        <property type="protein sequence ID" value="CAD8197466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X902"/>
    <dbReference type="OrthoDB" id="287110at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8197466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="152" mass="17660" checksum="D7B8B89F5CE7E5DE" modified="2022-10-12" version="1">MNTNKLRMSKSLIRQRQSPIPPLEPKLFVGQPLSQSPPQQKIPQMTFQQRLRLYYKSQNTLTPKPWNPPSDEYGTILRLISVHEVIKPKKKELQSRIPNISCRKTNIQKRFVTDMPKIQTDIANAKKIRLNFKSPINVLNKSNSFGPWDSPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XFZ6</accession>
    <name>A0A8S1XFZ6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Core Histone H2A/H2B/H3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PPENT_87.1.T1220150</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the histone H3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000122">
        <property type="protein sequence ID" value="CAD8199791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XFZ6"/>
    <dbReference type="OrthoDB" id="652632at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22911">
        <property type="entry name" value="HFD_H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000085">
        <property type="entry name" value="Histone H3.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45810:SF1">
        <property type="entry name" value="HISTONE H3-LIKE CENTROMERIC PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45810">
        <property type="entry name" value="HISTONE H3.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00959">
        <property type="entry name" value="HISTONE_H3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="9">
        <location>
            <begin position="45"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAD8199791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="137" mass="16099" checksum="F3D3F0699F0AD373" modified="2022-10-12" version="1">MARTKQGKPNNNKFSLSPQSFCNDSLDNKKQAYKNQQKRKHKFRPGTVSLREIRKYQSSTQLLIRKLPFQRLVREVSQRSEKDFRFTQSALYALQEATEGFITHLFEDANLCSHHAKRVTLLTRDLQLAIRIQGIEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XI51</accession>
    <name>A0A8S1XI51_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1250003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000125">
        <property type="protein sequence ID" value="CAD8200534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8200534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="781" mass="89852" checksum="CEE9B57097D5BBFD" modified="2022-10-12" version="1">MASKNQRKPYQMHCLISSQPYSFSQIIFENSICEANSSVSTTKKYSKQNQVTSYFYICQLKLYCIQRQKTYSLNYKNTLKKQLILIYIDLNSTYKTQQKIISSNHQFKYFPFKKGNLFPFRNRIKKSQKSIQTFDQYIDESYHRINGYIDSIYEQMYLKPAPEQKKDQRTSTELVKLPDAPKVKIEVIINITPDETKSFRCLQQQKVEVVVQEEQEVFERQEDLLQILSNANQIIIDDDDSSSIYKKLLDMLKKEQIKDCVAYLQSFKNQDQKEKEQIKKIINILKNLKDHEFNLKDYSSEAFEEIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLLKFLVLITSIDDKFISCGSNSLHMLVMMKVDMRNKSFENIRMKNTSLIGGNFVKCNMNGSEFENVDISVVNFNGAFVNGRTLRSIQSTICIRKIKIFIKQTYYMGLFGMLLSKGINRTFRGGITLYFYWMTREQYTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XIL7</accession>
    <name>A0A8S1XIL7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein phosphatase 1 regulatory subunit 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1250084</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000125">
        <property type="protein sequence ID" value="CAD8200694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XIL7"/>
    <dbReference type="OrthoDB" id="7451790at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050836">
        <property type="entry name" value="SDS22/Internalin_LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46652">
        <property type="entry name" value="LEUCINE-RICH REPEAT AND IQ DOMAIN-CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46652:SF3">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8200694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="339" mass="39637" checksum="818ADFE47A1110CD" modified="2022-10-12" version="1">MNQNQEDDAQPQLLDNQGTENQKQIEKKVVVQPFEQEKQKIQYKYLGHPPFEINQEDEDISCIEGRIPNIQALDKCVNLKRLCLRTNLISKLEGLQNCILLEELDLYDNRLIKIENIELLVNLEILDLSFNNIKKIENLENQKKLKKLFLLSNKIKKIQNLDFPELTMLELGSNKISEIENLDKLPKLRELFLGKNKIQLIKNLEPLANTLELLSLSCNKIQMIQPEIQCLQNLNYLQIAENFITIIENLNGLKQLELLDLAHNKITKVQGIDQLQQLQDLWLNNNKIEYFKDFELLKLNQSIKTIYVEQNPVSQFPDYKMQLLSLIPTLIQIDAVRIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XIN4</accession>
    <name>A0A8S1XIN4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Signal peptidase complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1240067</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SPCS3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000124">
        <property type="protein sequence ID" value="CAD8200332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XIN4"/>
    <dbReference type="OrthoDB" id="282762at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005787">
        <property type="term" value="C:signal peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007653">
        <property type="entry name" value="SPC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12804">
        <property type="entry name" value="MICROSOMAL SIGNAL PEPTIDASE 23 KD SUBUNIT SPC22/23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12804:SF0">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04573">
        <property type="entry name" value="SPC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016089">
        <property type="entry name" value="SPC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8200332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="185" mass="21964" checksum="11A5EA9A699C674E" modified="2022-10-12" version="1">MYSITARFNQIVFFTSWTLVVLSLLNQGTAYFFKTGKPTVDLKILSADKFHHFKATYYNGGVTDWDQVTFKFSLEADFEPVYNWNLKQLFLYVNVHHEHQDKGYESDCVIYDKIISRPDDPNSWSTSSKLVLKNQRAEYPLKDIHKQLRNATVNFEVWIEVMPYVGYIRREKLGDFEYKMPQQYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XIW1</accession>
    <name>A0A8S1XIW1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1250131</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000125">
        <property type="protein sequence ID" value="CAD8200784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XIW1"/>
    <dbReference type="OrthoDB" id="306433at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="168"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8200784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="188" mass="22507" checksum="9112ACB4C4719F72" modified="2022-10-12" version="1">MFKFESIIESLVEMKMQKSKSVNNDKELQKEELTQQIICFIEKIIENTIYMYIRPIEEQLRSQETQILQLQSQFMSLHHPFSPKNKEIVKIEKLESPIRSNEIQNKMIDLETKVQSITKQQETYNDQLIEFKQENKKSFYSYWVLLNDLNENVNKIKDFLSTLDLGEQNQSQKNNNNKLSRSNTKKDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XQI7</accession>
    <name>A0A8S1XQI7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1330183</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000133">
        <property type="protein sequence ID" value="CAD8203405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XQI7"/>
    <dbReference type="OrthoDB" id="308417at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8203405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="332" mass="38788" checksum="0502180C6C157324" modified="2022-10-12" version="1">MQTNISGFSEECTLVVKEQKQFRYEVLDQTQLIQKQQCNSLAFDNTSTLIMASFGMILKAYLFNNEKLIYISLFQITKSTVTCIESLKQGNFFLFAMLNGQLQISSKIGINQGKYIQKLNYHLSPIYHLKSSKYSDSIISCDDWKVSFWIKQSLLQFEYFYTNNIRICAVSFNEIGDKLILSDYNKSIVTFIKRGTQWMKLQNINIGKLGQGISFIGNDSFVVQFTFKKLDLYKFKNQDNQLIKLRELNIQSYGNWGSRKFNQSYIKEKQILLNKDGVSLNFIKIQDDPYFVTEFQLQFQDSSAQVALNENGTYLVTWDNYSKSINIYKYYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XSB3</accession>
    <name>A0A8S1XSB3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1340006</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000134">
        <property type="protein sequence ID" value="CAD8203452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XSB3"/>
    <dbReference type="OrthoDB" id="323031at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8203452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="203" mass="24321" checksum="23857EDA679B2465" modified="2022-10-12" version="1">MKILTKRFNLQNQELQIMEQFTQILEDLDNQNIFESFTIANSLEYINQVSIFSKKYFKVRYDANFIVSGFRINQFTAFDFYEIFLDFYHNQQLQMLKIQEKISNNKIFYQIYKIFQYFSIFLAIDMSKRQLIKLIITKKENYLFNQLKKREILKSLGKIHISVIIKMILNTKHYQENACQLILKAVYILSIINPSGLTDKSKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XVL9</accession>
    <name>A0A8S1XVL9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1390154</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000139">
        <property type="protein sequence ID" value="CAD8205133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XVL9"/>
    <dbReference type="OrthoDB" id="286841at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="152"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8205133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="521" mass="61942" checksum="B4BC9E034AA71CC0" modified="2022-10-12" version="1">MKQTKKRVSYIKNQKPTNIEWILNLLDQIPKYQLQQSNMKKIQTTQLSKLQDCDQLLKSSLYTFDTKINKFTQMQFTLYPNMLIGDNQEYLVLSSCIMNKKIIQYKEFTAQGVQLLNSQGNLFLFYESYVQQLNWEKQLKRFCKLQNFSNKYTIQEKLSIQNHYACVKNKNNRVYTVQLIRLSSLSEQLYEALMNEINILMCFKQNGLNQFHSLFEDGDILYILYDYWVGDTLYKLIQQGFKLTASQIAQIIFQIIKVIRFLHQNNLYHGAILPTNIILHRVTTPQNQQGQQLKITLFNFAYRDANQYNLTWLNKRVPKQWIPPEFNNLQIKSDFSNIDGYQIGVLLYYLTFHTNKNKSQLSFYKSLDNFKFDYQWLKSIKQEQQQEYSFSLIELLCGLLDPDYQNRITLNNALAHQWLTNQRQKQNVQNEKPKILPSLRTILELREQSSCEMLSSRLGKEEQHVTSSSEDDIEFCDDEYIHRISKENNKIPYYQNILSLIPNKHPSRSKQVIDQCQESQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y018</accession>
    <name>A0A8S1Y018_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1440142</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000144">
        <property type="protein sequence ID" value="CAD8206655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y018"/>
    <dbReference type="OrthoDB" id="304009at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="232"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="365"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="442"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="549"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="695"/>
            <end position="722"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8206655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="755" mass="89355" checksum="7F389051846C7BEA" modified="2022-10-12" version="1">MFKGRLNKDLKILIYSTQFQTLTFIIQNAAIIVRGEDNLILYQFNDRILFDLIYSQYKIFIIDLSSTPLKSILKCRCRDIQEIRERIDLNIQYFEKGNLRLQIYDGQIIFGEATINLGFYIENSLPVIIDNITIQSKYDQEAYVEMSLGWNQLEQIQTKEIQQINPIREQPLFIKLSQQTANRVPIEIYHKIENRTMNKICSEQNYFLENKQEQIDNKSIEQKKQLEDEKIEENPKANLETANSQRFQTPKDNKFAKKQKIQKSSGLLEHYKKQINTRQTYSDSKTNVKEQGLRILQKSPNDQGVKIRIHSHQNEEYDVNKGITKQKVICKKAVVTEDQGKYGQKHLEPGFFTSRQLEIDQQIEKECQQISIQQLLNENQLMKNQIQKLSLENQELKDQLIKSEATFNLLSETYTTLRNEYKKVQIKNYDSSNNTFIMNKEYEFIKKELEQKQKEIEFSKKETELQKIELEITKRENIQLQMDLEQIKKEVINKNIEYNDLLKDIQDKQYKINTLEQDLLNQQNNQTIFQDINLQNHLNQENEYNNHNLDQMKNIIKNQRIEIDNYYQELKQTKQNLLFQEKGGKQQSKCSEELQQINLKLELEINQLKQKLIESNQEIKSKALEIEKLKTQINLYLQSPDKQNFDLTYLINEYSTLNASLKQENQTLVHQNRKDQVKLESLQKQLGINKRKNIVELSEQRQQSLEKEILQLEKAVLNGKQNMADVINAVLECGGPDLAEAVERFLVIRRSMKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y1M3</accession>
    <name>A0A8S1Y1M3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1430116</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000143">
        <property type="protein sequence ID" value="CAD8206272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y1M3"/>
    <dbReference type="OrthoDB" id="296499at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8206272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="254" mass="29766" checksum="1CA1B8A87AA44C10" modified="2022-10-12" version="1">MNNAYYVPSYQTSYHIQQQACLNRNPENIADQLKLLKSKKHVNQSLHNNKDYLIKQHQFKSQKNISSTKLHSSRRQKSQDDEQTSQTINPTIRQLSKQSKRMNGYLIKQQLNSNSYQSHKQQQITDYHTVNKDKTIIKTEPDTQLCSDRIICYKTPIIISKQIQSKEQIKSICNQIYPQNKPVDEITNLKLDIGNENQRKNPKLKTDVQEKILDLLMLSTRELKIKLSDKRTKQTSIPKTTKAGLPVDFFYFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y4V6</accession>
    <name>A0A8S1Y4V6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1500045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000150">
        <property type="protein sequence ID" value="CAD8208263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y4V6"/>
    <dbReference type="OrthoDB" id="309379at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8208263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="201" mass="23053" checksum="E6726AF93625D27C" modified="2022-10-12" version="1">MNQILPNINSKTNALLQLIFLTTAGLLLCILLFSTYREEEIHYNGELLKCKLKYDAKMKCQLNNRTLNNNKITKEDCQLEYICNSILATYPITIIDGVLIIVTIVMSLMKHSNLPRNITTFNFVSFISILLSASTLFIMSIILMTGAQNENLSIWILVIQLLISLILFTSLIFIKKEQEFSNLIKKQEEMNGTEIHQYVIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y6F1</accession>
    <name>A0A8S1Y6F1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">40S ribosomal protein S4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PPENT_87.1.T1530035</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the eukaryotic ribosomal protein eS4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000153">
        <property type="protein sequence ID" value="CAD8209041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y6F1"/>
    <dbReference type="OrthoDB" id="1109245at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06087">
        <property type="entry name" value="KOW_RPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000005">
        <property type="entry name" value="40S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.740:FF:000001">
        <property type="entry name" value="40S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00485">
        <property type="entry name" value="Ribosomal_eS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000876">
        <property type="entry name" value="Ribosomal_eS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032277">
        <property type="entry name" value="Ribosomal_eS4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013845">
        <property type="entry name" value="Ribosomal_eS4_central_region"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041982">
        <property type="entry name" value="Ribosomal_eS4_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013843">
        <property type="entry name" value="Ribosomal_eS4_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16121">
        <property type="entry name" value="40S_S4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00900">
        <property type="entry name" value="Ribosomal_S4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08071">
        <property type="entry name" value="RS4NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002116">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein eS4 N-terminal" evidence="9">
        <location>
            <begin position="3"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Small ribosomal subunit protein eS4 central region" evidence="8">
        <location>
            <begin position="97"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Small ribosomal subunit protein eS4 C-terminal" evidence="10">
        <location>
            <begin position="214"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAD8209041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="266" mass="30176" checksum="4A2D7FB9D109B359" modified="2022-10-12" version="1">MARGPKKHMKRITAPKSWMLSKLGGNWTTRPSQGPHKLRESIPLSIILQHKLKYALYARDVQMILADKDGNIKVDGKVRTDFGYPVGLLDVITIDKTRESYRVLYDVSGKFILKSIKPDEAKFKLVKVTQKKVGPNNVPYIVTNDARTIRYPNPEIHVNDTLKIDLETGKVVDFLKEEPGNLCYIIGGNNIGRVGLIQHRERHLGSFDIVHVKDSNGKHFSTRINNIFTIGKGKKSLISLPDDNGLYLTALEKKQAREHQEETKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y9J0</accession>
    <name>A0A8S1Y9J0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1550069</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000155">
        <property type="protein sequence ID" value="CAD8209607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y9J0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8209607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="68" mass="7965" checksum="F0948DCA1AFE1B0E" modified="2022-10-12" version="1">MVDLKKSKETLLTAHEDTNTIQSDVKERALQQSIEIRIDQSVDFKEYTFDILQNMINQINGFQYVSIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YB66</accession>
    <name>A0A8S1YB66_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1610108</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000161">
        <property type="protein sequence ID" value="CAD8211009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YB66"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8211009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="156" mass="18408" checksum="5ECDDF363FF20FEC" modified="2022-10-12" version="1">MSEIDQLFNFVIQQKQFPIQNNLVNKQKRKKSLRKLCIIVNPYIKYRLFHHQSLYLTILNKLANTIFPQLKLRQKSYSKLNQKLSSVKVNITDQYDFIAAQQGLACSENGFLLNQNIETDFKIVYTPASTTLEKQIRIIEENELQQIIDLNFHFTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YBF6</accession>
    <name>A0A8S1YBF6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1620034</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000162">
        <property type="protein sequence ID" value="CAD8211090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YBF6"/>
    <dbReference type="OrthoDB" id="291503at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035938767" evidence="2">
        <location>
            <begin position="17"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="286"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8211090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="368" mass="41905" checksum="CC801950E18AC481" modified="2022-10-12" version="1">MQKLLALILSISIVYSIDPSMVQLSTEKIDELSQNNLGKFIIEMAQTHAEMRGPLDDLVTAIGDLENELTIELQQLEDDFTRSTNQHQVTQENLDINIGQTEINIFNQKDFIDAILLPSIDQTNSKIERLNGFINDNRDSLSRETLARQNQHQAYLDRVSEHQGAISAVDEALQLINSLINGNISFSEKATIHQAVKRVNTKIQKTSVIHPLVEALLQLTQNFSDQGQAKKIKDLLQDTRNQLVSSLNQENTDEQQIQETWEARQKTLNTEYQEFKRSVLEATYVLAAYQNKLKSTQEALAQNEQDLQNYQDSLQQDKDAQAQEIQIYNELKAQYQIQLQTTRNAKEFVNSAEFSTVIRNKLNSGGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YN90</accession>
    <name>A0A8S1YN90_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1720004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000172">
        <property type="protein sequence ID" value="CAD8212892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YN90"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8212892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="113" mass="13574" checksum="AF60120CFF0FC55D" modified="2022-10-12" version="1">MIYPKNKPQWQATIIQKYGLDLAEQINSQENIYLNKNYNFNCIQNLEQEINGQKALPQLQQQQSSNQLKQKDEDDQNHLFLKFQELHQYPNKTEKNREEKKNKKMTGVTYQEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YNW5</accession>
    <name>A0A8S1YNW5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1710066</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000171">
        <property type="protein sequence ID" value="CAD8212864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YNW5"/>
    <dbReference type="OrthoDB" id="284782at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="58"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="103"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8212864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="348" mass="40549" checksum="3551689AA58EA936" modified="2022-10-12" version="1">MSQKQLYNYKLIPEFSYKQHRHNTALAINYSNTMLFVADEITILVFQFKNELKKLQQVKSHSSKITTLNVFKQQSGLISASHDGSIILWSQIINPNFKYLARLKGHTDQITCLVLHPFSNKLIISGSNDKSIKFWTFNYSQFQNQSSSSWSCSQTISRHNASVSEISINQDGNKLLSCAEKQIMIMNYSDSNQWQVKQLIQISDVGVRLSFINDYLFAYQPWSGKSIHIYILDSIEEEYIKSREIPIQGEKDPCWFYFPSLYVPSKNILICKNGHKVNVLQFTFSQFGWKCNLEQSIDYGHNWIFGTLSEDGQYLITSDWLSKSIQIRQLTQQEEIQQFVKNNINYYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RRT0</accession>
    <name>A0A8S1RRT0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FHA domain containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0010169</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RRT0"/>
    <dbReference type="OrthoDB" id="10261999at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00060">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000901">
        <property type="entry name" value="RING finger protein B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000128">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000081">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000114">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050923">
        <property type="entry name" value="Cell_Proc_Reg/RNA_Proc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23308">
        <property type="entry name" value="NUCLEAR INHIBITOR OF PROTEIN PHOSPHATASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SAM" evidence="4">
        <location>
            <begin position="17"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="183"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="287"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="378"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8131571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="425" mass="48837" checksum="4BC9FBAAB6C4B68F" modified="2022-10-12" version="1">MDYETLFTILPKDPKKWSLEDVSQWLNFVGLQSLQSTFTNNSIDGSCLELIEENDLIDDLGISSKIVRKKLMHLMIDDKRCDKMEQEYTSLENTEQTQPQYGQINVSHDMMTNYIKNNEIYSQQFQQPLCEIENKPQFLKKQQTGIQQEQEVENLVSKVQNELIIQPTEGPQTNFYSIKESGGKIGRHSSNQILILEESISRFHAEIVFQNEDFFIKDISSTTGTFIKVESKLELEIGMVIELGSNQFEIQQLSMNNQIVEVVMLIIEGLNSSEKHIIALNPQKCVTTVGRKQTADLTFSEDHHLSNIHAKICLIEGRVYLEDMGSTNGSWLRLSKEGQFSQLYPLQNQTVFKIGTTSTYLCKRTTQLVTDQNNENNCIICIENDRDALYMPCKHNTACLKCSKNLKDCPICRTKIQDVIRIYKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RWA1</accession>
    <name>A0A8S1RWA1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HIT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0010551</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RWA1"/>
    <dbReference type="OrthoDB" id="295085at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23024">
        <property type="entry name" value="zf-HIT_ZNHIT2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007529">
        <property type="entry name" value="Znf_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039646">
        <property type="entry name" value="ZNHIT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15555">
        <property type="entry name" value="ZINC FINGER HIT DOMAIN CONTAINING PROTEIN 2 PROTEIN FON -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15555:SF0">
        <property type="entry name" value="ZINC FINGER HIT DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04438">
        <property type="entry name" value="zf-HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51083">
        <property type="entry name" value="ZF_HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HIT-type" evidence="2">
        <location>
            <begin position="33"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8132318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="380" mass="45481" checksum="875E628C4C8C18F1" modified="2022-10-12" version="1">MKQKENNESDELLILAQKSKLLVPQFKKNQQQCDFCQKQMTKYSCLKCNKMYCSLDCYKTHSETCTQSFQKEHEIQKMKQMKASFDEQIEIKKALNQFYEESKDQNIIPINEEEINERWEELQELYDQGTLNLDQLTLVEQHEFAKFIQEIVIQEPWIPWWEVNDGIFSLYIDVLDQNQNQNQKINKDIYENTYLEFRSTLKQKIQTVVSFDKLCKFQHEDLRNHLFNIIASISIIAKVLDGDIIELNREFYNMFITLSYSMDDNTSLIGNLDLSYKKLKEYAHKVDKGLILPYINKEDLIKIFSDKFFVIEILFYAYDAIHSIEHQAQNEDNSDSVENKNLIKTLNLKKQKLLFYISYSLSKNHEFYWQIRTQIKELNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RZ24</accession>
    <name>A0A8S1RZ24_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0020293</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RZ24"/>
    <dbReference type="OrthoDB" id="267284at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008496">
        <property type="entry name" value="TMEM222/RTE1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20921">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 222"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20921:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 222"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05608">
        <property type="entry name" value="RTE1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8133218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="197" mass="22676" checksum="75C39AD7269B69A6" modified="2022-10-12" version="1">MGAMALSQAQNHKLAVQDENQVQDVEKENMEIEQVDQISVENQKYPFCIVWTSIPVLTWLIPCIGHTGICTSEGTIHDFGGPFYIAIDNFTFGKPLKYVKLNKDFEISRQKWDDAVLKADDEFGQQMHNLFTNNCHSHVAKALNIMKYKGKQSFTMFHIWLMLIVSGQYVSFGRFFMTYLPSIIFYGIILMVVFLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S2Y9</accession>
    <name>A0A8S1S2Y9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0030063</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8133962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S2Y9"/>
    <dbReference type="OrthoDB" id="306416at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007858">
        <property type="entry name" value="Dpy-30_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05186">
        <property type="entry name" value="Dpy-30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="3">
        <location>
            <begin position="391"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="3">
        <location>
            <begin position="514"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="134"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="243"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="282"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="154"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="191"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="248"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8133962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="639" mass="74331" checksum="D0A2329EF3FEE4D5" modified="2022-10-12" version="1">MEEQDSRSYLASKVHPLFEKLLIELLIAKPEDPVEFMTQWLVTTGQQYRPQEKQNVPFSVTLPVQSHNQMQAGTPRRSQVSSLQNPLLNRQQSNQPGSANHIPNLQSNQQLSHQHSNQPINSQNEIISQQLIHQNHSKQGLEQSDIQQKSFEGSQHEVEHHFDQDPKFGSGDQLSPSPIVADPKKSAHPFQKQQSVLTQKFQQPQSNMNKSFKKSSTQSNPMYLQPVTMKQAQPQIMVNNTPREQSLQQMKSSQVNSVQQSQQGLIHSRDEQEILDNQLIKRNSLTESREQLIKQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQALSQIHQAQQQESILSNQHQSQINSLINSQKNHKVIPKNKDQKSRVQKKINTCYLFKHLNDQQKEIVANAMEEKKYKATETIIKQGDDGDQLFLVDQGLLDCKQRNKQNEEKIVKSYQPGDFFGEMALLYNEKRAATIMAFTDCVLWVLDKETFDTFIKQPVLEKRQRYDGILQKFPILQQIDPYLRQQIADALHVVSFKAEDVVFKQKDKGDYFYLIQEGQLKAFKKGEEDEDEEQCVFEFCEFDYFGELAMLKEIHRQATIVCETDCILLGLDKQSFTKLLAPIHDVIKQGTGRYLNFSFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S4K6</accession>
    <name>A0A8S1S4K6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0030024</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8133884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S4K6"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000171">
        <property type="entry name" value="Eukaryotic aspartyl protease family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000104">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF51">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035917576" description="Peptidase A1 domain-containing protein" evidence="6">
        <location>
            <begin position="19"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="7">
        <location>
            <begin position="89"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="292"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="120"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8133884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="418" mass="46805" checksum="9CD3E5B59DE0B3EB" modified="2022-10-12" version="1">MKLVLGLIVLVTLGSCASFLEEQQPMENKIYKIKLDRTESQARKSLFDFITTSQQYRNSPDLLGESDLEMAQTKQKESIKLYNFKNTQYTGEIGLGGQDNKFKVIFDTGSANIWLNSARCNDYGCKNHKQYDGSKSSTYEHLGYDLDVEFGTGELMGEINADTAYVGGVKIAKQEFAEIVRENGDVFAQSDFDGIVGLAYPTMAAYNFNPLFDNIMQQKLLDRNVFSFYFSRQEGSRSSELTLGGWDNDHFTGELHFHNVVNKYYWLLDADNILVNGQDVGLCKHGCKVVADTGTSLLTGPSEGLYDLLDTLNIDENCSNVKELPKLTFVLDGINYDLDANDYVMKIDSQGNEVAYDTFASTDSFVEMGANCQCVGSFMPLDIPSPQGPAWILGDTFLSKYYSVYDRDNDRVGFARSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S4M1</accession>
    <name>A0A8S1S4M1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dual specificity protein phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0030034</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 11">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8133904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S4M1"/>
    <dbReference type="OrthoDB" id="10252009at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45948:SF2">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45948">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE DDB_G0269404-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="6">
        <location>
            <begin position="16"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="7">
        <location>
            <begin position="76"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8133904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="153" mass="17497" checksum="C8DD546DC41C6BBE" modified="2022-10-12" version="1">MLIQEMKQNCSLILKGESEDKGNLYLGNLNSIEEDNLKAKFIKAVITAARGVNTTIKNVNHYIIEADDDDNFQIIQHFQKSIKFIEQNLKSTNVLVHCFAGVSRSATIVCAYLMKIEKKDSETILIKMKALRHQVNPNEGFRNQLKLFEKKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S6K7</accession>
    <name>A0A8S1S6K7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0040494</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S6K7"/>
    <dbReference type="OrthoDB" id="306501at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8135926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="202" mass="23104" checksum="F4C00742B843DEB4" modified="2022-10-12" version="1">MRTYNDYSYSRPLDANFDLKSYRSHVKDRLNLSLNNDLNQSNFNSVKKENRQGVPSFDNLQARIRDLSQSRMTTLNYNSNDDYTPQKNLYSPHRAYGMKQIISSNLGQNVVINSNEKQSQNSKSQSSQRKLFQRSNDNLQLSLKGSQKKQGRLLSDAVQINEIVALRNKIESSLVSRSSLTSTYISELVKLAQAITTSLKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S8L8</accession>
    <name>A0A8S1S8L8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0040373</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S8L8"/>
    <dbReference type="OrthoDB" id="288288at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="489"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8135710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="589" mass="68732" checksum="4CA2C33A91656E85" modified="2022-10-12" version="1">MSTKNINKLKLNSIMSPASSNIRISKNTALQMSSNGVSPKMLSKSPNYSQYIQHQLSQFYTPPKEKLSKIINISQSERSVYSFRIPTLRMKEKCFIKKQQQYKLGQTSFEISLSTNTSSIFIFKGSIQEISQFKVIQMTRAELQAKSSSIYKARQNLLQKIQQNLNVPKSYTQLSLGNYEAIQFMEEIPINCNVLLLSKTVNKDFWNIILNYHNFQFTTQKYTKILTIISKIKDLNDLIKFILDKDVFYTYMELKEIYLNEDLLTMKRNTIIMKDFYKSINVYDQEKQQLIDEPVTQKQMDNDSLKVELDKEMKKDFTLLNLNPNPNDFQLILEKEGLLNPSNRRTSSRYLKLPNFRTQYGEILKQQTIKQKHKNKYTNIPDHIARNILHMTHYNLPQLMKETGFDRQEIYEVFSRYKALLSYECSQIPGLTKEMIPNGISREAFNAGLEELSMAPPGVVDQIFKFFAKQNSLGFEGFLKAINLIRAKGNDNKIELILKLIDENENGLLSYEEIKNRCSFMMEEMLKDSKGELSVNNMIDYVTRSIFDAVKMKYDEEIPIDKIRKLIESKTHEAQVLIMMCCGDVDYLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SDC4</accession>
    <name>A0A8S1SDC4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0050499</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8137092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SDC4"/>
    <dbReference type="OrthoDB" id="10361675at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="325"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8137092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="347" mass="40940" checksum="9214A98B8CEC7248" modified="2022-10-12" version="1">MILSNKRKQELFRLDSMLDKLNEFIQNQSLQLENIQRIKTIESIDQQKQFQFKIKPLEITILYEKLNSLLLKKKSSIFIHFLIVTKNQTASKVKYNMQESITIKCFTPRDMNQKSLNCINSGCNKQQISSNLSLSQQSYNKENIGSTRSAHQNSQLRSNCIYYQIDTIKENNKEKKKDFLLSPSDTPLLNQKTTKYNFLKNKIYTPSYNKQLKSSYKSYQTQSRERVFSKNFRKGLLQFDDNNLNNYQITQTIISNQQNQISYFSIETSTPEEEDQQDQLFTNPSEWNQYLISRDNKFVSSRQQTCVNTQTQQLQRETMNILKSCLFGFFLGLFLGLVLLKNLYIFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SFL9</accession>
    <name>A0A8S1SFL9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0070233</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SFL9"/>
    <dbReference type="OrthoDB" id="285625at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8138650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="177" mass="20825" checksum="9C1F3B71D996B2E9" modified="2022-10-12" version="1">MQEKQNEIFEILQKQVDERKQKKKQEMFQLKKQKIAEIYQDKKKLTDGLAKHPYQFYPGKGVGQFFQKSDFLLPKQQKSGLERIQSAWLPTYTHSRLISATPFDTEPFPRPPSVKEKVWRPFSSQPNKLNMIQLVPHSPKEDALTPKKNSMTFDTGEKLVQMKPFQPTRRIFSAFPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SGS8</accession>
    <name>A0A8S1SGS8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0080228</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SGS8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8139655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="36" mass="4068" checksum="814D0D216B3B1F67" modified="2022-10-12" version="1">MRQISSALQLKTNCNQEKLLESAGQYKLVDSKSINQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJH6</accession>
    <name>A0A8S1SJH6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0070380</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJH6"/>
    <dbReference type="OrthoDB" id="184876at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005301">
        <property type="entry name" value="MOB_kinase_act_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22599">
        <property type="entry name" value="MPS ONE BINDER KINASE ACTIVATOR-LIKE MOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03637">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01388">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8138922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="207" mass="24327" checksum="F54B34BB4143B475" modified="2022-10-12" version="1">MRRNRIGTKAEFLWAWDVEDISQMNGPLAVQEYIQELIRADSSNIKQIITPPSEVDIHVWQYEHLRQFILELNLLVTQLKGLCTAQTCPKMKATEDWLYLCAAHKKAQECSAIDYMIHNLDQSTSILTNIKTYPSRVSINPQNATNNFAFIVRRLYRLFSHTYFNHKEIFEDFENEMFLCTRFTEFALKFELMSPKLITIPKEALKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SM22</accession>
    <name>A0A8S1SM22_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0090338</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SM22"/>
    <dbReference type="OrthoDB" id="2021138at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="43"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="183"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8140900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="407" mass="48149" checksum="6E57CA5FFA80186B" modified="2022-10-12" version="1">MNISENDMHQLIQSVNKRSMFKTQYDIEFIHSKLRDFTFFRNMNREIGDSQYYKILKELQYECPIPYEPLVNIGDQCSKFYFILAGRFLILTNNQIANIQLPTEIQKTLEKLFPNQIPQGYLSIGQYFGEQVMMFDQQHTQTILPLEKSHLLVIKRETYRRIIDLETKKKESERFSNLSTIPLFQKWSAKTVKMLSSEIQELNFIPNQIIYHQGDPVDSVYIIIDGQIQLFRTSNKNQLPLSILGCKESFGDDEILTQFRTHSAKSLTSVKLYRILKKPFLDLIPFDYSNKYFKKDTHLKLNLNQNEDYSRIYRSESLKAIKSPAKLLQRIMRKSQDKNKTHQSISFQQIHDFTRKDIRYSNTPTVLQKSSSVHEQIRIHANGKFDKTTYLLNQIKLRSTNVWKKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SPK7</accession>
    <name>A0A8S1SPK7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0100281</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000010">
        <property type="protein sequence ID" value="CAD8141738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SPK7"/>
    <dbReference type="OrthoDB" id="286314at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8141738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="201" mass="23989" checksum="DA79CCB0A98A0C78" modified="2022-10-12" version="1">MFKNQKIEKQRRSKERFIYEACNLVQQWRDLFKNGYTDYAGNFIKPTLKEAANLIGCSKKTLEDYYSIIRKASQITDINQCLQKKMGYLRSLLKQKEENLNVAAPVIEEIEERNPSNDSWNNLIIEANDENQEDSNFNTDRYIIETQIECEGSPILYEREQFDYEYNHTEINSNQYWKEVQIEDDENDLSIANQEQIFFDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SST4</accession>
    <name>A0A8S1SST4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0120478</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SST4"/>
    <dbReference type="OrthoDB" id="346907at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:002024">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="20"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="292"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="292"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="316"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8143935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="521" mass="60641" checksum="347EEDD2AA70F2B7" modified="2022-10-12" version="1">MNRQEIINIKPEKKISNYAFNLKAILGKGSFATVYFGREMNTQLPVALKVIDHSKSINYAQLYQSLHQEIEIMKKLKHPNIVQLYEVYSTSNNTYLVQEYCNGPDLKQYLSEKKVLEEEQAIKMIKQIANGLKEIVNNNFIHRDLKPANILIHDGQCKIADFGFSRPLPLECIMESLVGTPLYMAPQILTKQQYTSKCDVWSLGLIFYEMLFGTLPWMATNYMELIYRINNCKLTFPKNNKISKESLSFIQGCLHKDEVQRLSWNSVFLHPLIKPQMKIPIDPGINEIFSPKFSTHRQIQSNDKSTLPTLRERSCSGKNPQNKQSNEINIEQKQELPPATSSQQSTEGEENTKLGKYYLPKQQEKFRRTRSQFENIKPNQTEINPKRYIQQPLPSNCNIIKQTFPSKKRLHFELFQKNNAQKLKSNSETIIQLLDLITSLEKDVKNQDIDLEAFLNKERIAITNGQQNQALRIKIAQRYVEKLESSKKRKLETYISLLQKDQNYEENNLQKNNVAHLLYQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T287</accession>
    <name>A0A8S1T287_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0140330</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T287"/>
    <dbReference type="OrthoDB" id="305993at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="311"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="220"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8145609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="342" mass="40226" checksum="EF36EC196FEEA6BD" modified="2022-10-12" version="1">MNQVQYMQVLRDSQEIKLRDKFTQLQSKAHDYVIKQMKQLEQKYNQQLLLIVQQYQECKLMCEQLRLKNEQYSKLILEQEFKSIQTKNYMCLEVVNQLLTQKINSLLFQLKSHNIISESEYFSQKNTLQLQLAEIICNPNNHDQSTYIMPLNDTTKATLIVSQNIKYDDQFEVKNNNNYENTSIGIQTNLFKQKKEKSGKQQKIQIEEQQTISPNISNQLNECIKSIQELQKSKVDLQANNELLKSQNQNLQCRLNQKVNNSFKCNHSVINNRQIIKKFLKFDDMVFKNVTSFSMAQSFDLRQGNISSRQFFTRPKSSAPSTTRNRFNSTQQPSSTGFHVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T4E9</accession>
    <name>A0A8S1T4E9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0150284</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T4E9"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009190">
        <property type="term" value="P:cyclic nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43336:SF3">
        <property type="entry name" value="GUANYLATE CYCLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43336">
        <property type="entry name" value="OXYGEN SENSOR HISTIDINE KINASE RESPONSE REGULATOR DEVS/DOSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="4">
        <location>
            <begin position="729"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="78"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="356"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="286"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="650"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="104"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8146317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1075" mass="123838" checksum="9AB9C679DC124377" modified="2022-10-12" version="1">MAEREESMKYYHSRESENDEQKKPSYAMMSIINNQPHPVYFNGVSQRKSIENEYKHGPLLIDKPKCLPIIKKELSQEELDDQEGKVRKINSGSSIAEINEQKSNRSQSSAKSAKSAKHLSNQILNGEPKKKQIRRMNSKIKARLQKMQNIKQTSAFQKLVSQFIEGWAFSILMAIVTLYALFGDDIRILTVNKDQDDIFFILTIISMSFFTIEIILTSIANPNYILNFYFCLDVISTATMILDIGWVTDLWYGDEGDIGNAATIKALGRASRVARKAARVIRIIRLVRLVKLYKNARQQYEKDQQRKILQDILKQNRILSDENQKQQQQQQQQQQQQQQQQQQQQQQQQQQNYIIRNSSFPSPPPSGGQQLKNNDDKQELSEFQNLVKDTSKPFLGQNQQQQNHEMSALSVQQGLSYDQNAQGMSNEQFRQSQQSQQMNQNFQESNVGSRLSDLVMRRVITIILAILISIPVLTLDTYQETINSYDSGIFRIGQFRNDQKLLEILTMQYVKFHQDELFPILAVYVIRNNVTNNFPKLNETNFQIFNYSRNSEWFQSTHLNIDQHRFSDKQYYAAVDTNNKLITCSVGDLIDYNQTNAILSIFQTLFVCIVLASSAILFNKNVNELVIEPIEKMMGKIELIAQNPLEAVNIEEQEDLIMEELEQNEDHEKIKEKYIEKQMETYILQRLIMKVGALLAVGFGEAGSEIIAENIKKGGSVDPMLPGKKIMAIFGFCDIRNFTDATEVLQEEVMVFVNEIAEIVHSTVNQYGGSANKNIGDAFLLVWKYLPMEYHPNPQNPSKLIVKTDHHIKQKGDMAVMAFLKIITSISISKKLERYKKHAGLNARMKDYSVKMGFGLHMGWGIEGAIGSSFKIDASYLSPNVNMASRLEAATKQFGSIILISGVLKQYLTEDCQKQLRQIDIVTVKGSVEPVEIHTVDMSIKNLIQKTKEMKDKFDTNKMNQKQQKQFRVLNRFKREQLLKDVQKDKVNVADQFKTDEELLYSREPFTQEFYQTWKEGFQLYIKGTWDKAQLIFQKTLSMIPEHKDGPSNTLLEVIHSNGGKAPHDWKGYRELTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T612</accession>
    <name>A0A8S1T612_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0170213</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000017">
        <property type="protein sequence ID" value="CAD8147930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T612"/>
    <dbReference type="OrthoDB" id="310242at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="32"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="434"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="827"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="584"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="149"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="434"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="514"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="528"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="828"/>
            <end position="866"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8147930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="945" mass="112325" checksum="D62E076A30B05670" modified="2022-10-12" version="1">MTEAKSKRDLIWEEKRRQREERLLRMEGQMLENNANQFQLPPPRLDDQVPISFPSQPQEYEPYIPPEPYVPIENYRNPDPPRMYQGSIPQQSDFREQRDKNSQFQQQPAIQQSPLKNPIKQQQDEYREYLKKQMEDKEAEKKRRRDGTNNNQQQIQQTQFQQQSANPPPLPTQPINRQKQEYGEFLRQQMMEKEEQKKRRIQSGNNQNTQQQYQVQQYQVQQQSQQYQQQIYQPQQFEINQGGYSQVNQQSIIPYQQQQPQQINQNKQQKQEYGDYLRMQMQLKEQEKNDRKKNLQQRMETGDAFPFGKGGGGAPNQRGANIQVAQNYNQQQIYQQQIPNYQQQPQYVQQQQSFQQPIQQVQYQQSIMTPQQYQYQEQNIPFDQQPILTEPQGDPQLKGGRNRLMMENQNIDDIKKKELQKIEMQRVLQEQIEEKKRKKEQERLQKEMEDRQEEERFRRQKEQEEMEKKREDDLKKQQQGKFEQDNIDLKEKRRLERIQERKNRKNQQQDEDQPQQNFQGFEQQQQQLPPPSRNLPPSQPKSENYLKEFLMNEKMKEGVSPHQSILDFYRNGPQPMNKVEIQQNREIDDIKRQMLQQQENLQQQIQQVMSQAQMAIEGRNKAEQELIALKEQVKNKYLQEERHHDDLMIALVKNDPKWEKQVFPPHLLAQAEVGAYKDLSMPALNENFNFPNLSQNKAPPFAPAYESKPLVLKEESKEQKQNFREQFGLQSLVSDSNMVPIDHNASLVPQIQPMNKKVSEVQLDKIDELNAHFEDSLKLGIGETYQLRHDNYQKRPPSNNGQHPSWNVPSIDSEFVKEIDDLLKYKPAQQTLNRPPSGFTRNGLEQKQLQQSISNRPPSQTNQRPPSTFGRVAQGILGSADLNYKGITLDSEFPDMTMIKKGTNTDTMDIAGIHKKMDQKLMHLNEIEEDDDLKKLDKLLYQYNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T6A5</accession>
    <name>A0A8S1T6A5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RCC1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0160313</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="292250at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051210">
        <property type="entry name" value="Ub_ligase/GEF_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870:SF446">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13540">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="41"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="RCC1-like" evidence="4">
        <location>
            <begin position="132"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="205"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="257"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="311"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="539"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="615"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="668"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="742"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="138"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1014"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="153"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8147308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2022" mass="232020" checksum="013E6380116C4C7F" modified="2022-10-12" version="1">MKQQVNNRINPDLLFPRLAAGVNHCLAIQSEGDKTTAPQTFQLWGWGSNEFQALGKPESIDSEIQPSPVKIYLEHKQKPYVPLQVACGNQHSMILACSQEQFTIIFKNSQSMDEGFNYDQGFKTLRYDDCVIFSSGSDEFGQQGRMTDKEQSQEEIEEGEQEEDDEDDYNEQEECFRMKGVVESISKVCQISCGANYTLALDIYGSVYSWGEGATGCLGLPMQVDQQRPTRIDFDKIRMKFISAGPSHAASISEGAGSLFTWGVGTYGQLGHGTNKSSNVPMSVDAGSVVGKVVEYVSCGAMHTACTTTDGYAHTFGFNKNGQLGTGDFNDRWEPVKVHSIQNFYAVYAKCGGNTTFITTLERLTFAFGSNSKGRLGIKDEFGSNYPEPQRIDTNYFKSKQQFIYQLALSYHYGLALLQSGSIISFGLPFKGILGRDLQAELLPKNYLTNNSMNDDYKQGDDDEGEEMAQNKLNEVLNTIKPDLLQSLIEPKNINEEFCFFGMHCTEFINKLQKEIKASSYIVDVKCGAFHTVALTDAGEVWVWGSNKCMQHGLKDEQIIEQVKLRTKFVGPFIQETIADTSYPSQVLTFSIRENKRVTYLATGLEYVVVVENRRNIYSWGKNDKGQLGLGFVSDFVETPSMLTDLEGFMVKQVSCGEDHTLILDEGGSLYAVGSPVDGKLGLGPKNSVVLQTQKVPFIFDVAKVAVGPHHSMALVVDKKYNERVIKTKANEKNQEDNSQKYVIYTWGNGYGGKLGHGNLDNQYYPKLLQTKYSFKDVSAGTNHSGALTIDDQIVVWGVGSYLGIAKPEDDTDDKQHLKSDKDQEAEPYYIVSPIKHNDLATRKYKSLCLGDRYNMAISKQNKVFVWGLFDFDFQEKLKNQCANAAQREKYVEVLMTNIVNTPLCDIQFTTVSCSFNHAVALTENQDKKELFSWGYDGMTGRLGLGYEFINPDKENEMQLQNKNQQQVKKENFKTLKTLFVKPEPLQFINQYLDYTFKQYQLMQLQADKVDEDEDEFEEEFKRKDQKKAGTNTDGFQLPNKKLSKRPLSKRVFIGASLGMIEEGKLICKSSWDQFINESKPDIYRCSYVDRGDDPEKITTSRRVNFFEEKPVDDVYDAILELDQENQRLYKEIKDIHLQEFKKFKDEAMLTIINKVQRKPFDIQFKTEEKDKDKKKEKKQKKHEAYYNCSLCYKIMFTMLQLHPCYFINIFKHGGLSKKNFVDLVMDTFGDISNDKRKYRILINLLIQILRLELHSLTSKKLIFTQKETNEPSLYAFTNLFIQFQGQFGKCITTNLKLIETSIKSIENKLTSGIAKNLKPVALVFSQNHIQNPGCDVLLPKFDGPQESKFFQERVIFIKEFWDEFAPKLTNFLPQDKRGPYDVPVAVRELFLKTYEVVKPFFQKNNQQIEQIQCRMLGLYYQNYQNMFIAQAQKQESEKTIDQKAESNFLNIKCFLEMFDYFINDKEEEPENVPLWQNIIEIFGNHAPRRITAFNAIAYGASTTNGIQNIKLQEKIQMASKYISKNEVTKIKDIYFHAIEYSDDIIIPRVKSIYWATKSIVENIQKIKYVQAENQDLMEQFAKSIKTQPISYLTETEPDENTGSERIQVKLKTRQLYYTTMSLSLRHCSECGCYAIESFLRTEDQPPFHRFDQWNPVSLSALLIRIVSCFKNKKDFDEFLAGQGDVTTILKKKAAVQKEQGLFGNIIKLKEFEKAFDEKVQFNIEQSDEVVEDSKLARTKESKKVLRELRLLIQDRLISVYKYLYNMEKNMVALDAINFELQEKITKFKTEKVGLYNRIISNCYFGMANKELIKQLQSNPVAMVGQIKDAIKQVSTSTEIYENIDVFKKSADQPSYLTIFGSYSYSILQSRKIIDSIKSMAYDDIKRHTKQQVTPNVINNRLVFSFQKDINSNSLLIVIVYSEVEFDWKQTVSCGLIQPNIEPIEFIVDQFVVETTTILQMRKDIALAIDIEKPQPLGKYCNFRKKGLMQLLDELTNHAVIYDHLYVEPEKKDQHHHNQQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T8D0</accession>
    <name>A0A8S1T8D0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0160321</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T8D0"/>
    <dbReference type="OrthoDB" id="284369at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8147324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="154" mass="18639" checksum="39CA9DD5332B492F" modified="2022-10-12" version="1">MNQYSFCSPKPRFMQVKMDEAEQRSWSKEYKKRSDYFCLFSKPSTSRPQTVDSKVEGNKAIRCNSRKQRQHSLQDPDIACLNQDVQLVDYLFQLKNRQQDRYKSRILKLHEKKTRVWKNYKFPELFQSIKNRNDSFDPLIIFDKKKEPILNYIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TAZ6</accession>
    <name>A0A8S1TAZ6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0190195</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TAZ6"/>
    <dbReference type="OrthoDB" id="123929at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24115:SF1008">
        <property type="entry name" value="KINESIN-LIKE PROTEIN SUBITO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24115">
        <property type="entry name" value="KINESIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0493">Microtubule</keyword>
    <keyword evidence="4 5" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="8">
        <location>
            <begin position="28"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="723"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="661"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="727"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="747"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="111"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8149610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="771" mass="90369" checksum="91CE35C8B507CA18" modified="2022-10-12" version="1">MEVKKKPAIELPTVETLSVLDQNEPQNGIEVICRIRPKFVGENYVNYMITDKKLDIYEINNENEISNQKTFAFSQIYDQNSTQRNLHQEYVIPLIKDIFEKQKGGLVFTYGVTNSGKTYTIIGTQEAPGILPLTLSTLDKIKTAFLLNKTTIEIDGQVIKIRESPYDDYVLKEVNYTFQSLEIYNEEIYDLLANNKTKLQLKEIYDKKCYVKDAVEKEINNTQQFKDLLHKALQNRQMGETTLNNQSSRSHTIFKIQISFLYQQEQFTIQKKQTICIVDLAGSERAKRAETQGSQLTEACNINKSLLVLGKCLKQMRCQNESDQNCRIPFRECKLTRLLCEYFTEENRILMIVNVRLTGDDIEETLKVLHYGALSVNVNLLKSKIYDSTISYNQRLQINQSQQILNDSKSKKIPKKNAKEIIRRAKMLVQNTTQHMLFQQTIQDSFVKDLISTVKQQQARLKQFSGLDDELFYPPILESDQRLQKSLDKIQSDRSRQASLDILDNKGLFQPPPVSNFLQDIPQRKSYKFEEQINQNKDVIIDERLQIDQLKLSEIQEEKQDIHSIIEQHFNSYEEQEELINDLKQLDLEQIDEQTDDSKQQSKCHIIKQNNNNQENDINISNQCQYKEDLLQYSNQKYDKNHFDQATNTTPYDEMKKKLFGQLFEQELQKHQDEKDKFKQEVMKNLFNDDDLQNLIEKENQQELINLEDSAKKQQQRNIEKLISSNHKSDLKIITESDQEHDSSGDKKHHQTKNHPKKKKQTKKKGKSKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TCW1</accession>
    <name>A0A8S1TCW1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0180423</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8149116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TCW1"/>
    <dbReference type="OrthoDB" id="288416at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8149116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="184" mass="22218" checksum="48D00A048AD56269" modified="2022-10-12" version="1">MKKTNLKTNVTITTQKRQNKENTIKSPRKAISPKNFRQKLTCPDLWDNIEKQQITSETLFQQIFPNPNRKKLTYFQFDQIKFLSVEKNEIFELLQQDDWIEYNRAGKILGEKYGFEQSQLKQWFDENQVNQTIKKADLLKKYKEKSNLINKLFEEVDFNNTQELSYWDIYIHKDIYVLKQVIPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TI26</accession>
    <name>A0A8S1TI26_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0210055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000021">
        <property type="protein sequence ID" value="CAD8150866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TI26"/>
    <dbReference type="OrthoDB" id="295515at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8150866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="483" mass="57578" checksum="3948E0B1E2BFAA14" modified="2022-10-12" version="1">MNKYTLMFKYKQLEDEYQKQRINTISIPVFQFGSACIFIVATSKLITLVLQNELGLMPILIGCQVYSLLSFVFLRFKIHKINIQLIILAYLLIGYELIITTDTSAQALSLYQSNFTMCGVIIILISEFKESLVIIISTFFFKIIYTMLMETQKSYIIYCSTLILMFFMCFFSYKLNVLNRTSFLLSQYDYMWESVFPKIVDTPYLIFTFSDEKLEFIFKSQNKLPFQCDNSKQLKEFLREWQCNKNSLENTLYNLYTNQNINEFHSRKIEILKDNKTKQLIRYSMMKSLSSTFIIKFDDSVMKVEDCKVILKSTIKKQEELRMKLVKRIYKHLQMSLIQKQQNNLWLIRNSCLKQIMNFKILKYQWKTQLFDTKNLLFLNDFFSYKLNRLQIFNPKDENIITIAIQLKRLIFEILELTYNQRPVDVTILETVTITYEGKEISNKKDPVLKLIWSNLVEKAEFQNSCWVIQLHKEPCVNFTNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TK67</accession>
    <name>A0A8S1TK67_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0220287</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8152117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TK67"/>
    <dbReference type="OrthoDB" id="414534at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="5">
        <location>
            <begin position="121"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="245"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8152117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="421" mass="48554" checksum="15B96949BD4A7C00" modified="2022-10-12" version="1">MSDTESELLECCKCGAQVDQILSLQCDHNLCLNCAAKAVAKWGSFTMYPPLICQICSSKTQLDPSAVQVLLEMNPQSSCCSDDFEQRSEILETKQSQQIQHTDYHNQSQQQQQQQQQQLTNITCQQHLTESCVLYCFTCEAQCFCMECYLQGLHKNHEVRNISKSFDLIRENGNQLQVSLQSCCEYLLHEQCKVVNKKQDLVDLVGQAKMQITSNFEDIYKTLRLKEQEMLQAADELVADKLIEIESLMAKIKLQTDRVNQYNEQLVQYFGCTNPNISLCVEACNFVVKEKKKIQNLIEQVRQEKISPTIQGKFCLDSGSIQQMISDMRGVKLQLVSLRGIESINPTQREQYIYQKAIEDKTRDSMIGNNQILREEKENNNSISFIERSSQKKSIKSNDNRDFYTKFHEAKRSIMNQKSFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TMZ5</accession>
    <name>A0A8S1TMZ5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AMMECR1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0240157</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TMZ5"/>
    <dbReference type="OrthoDB" id="24630at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023473">
        <property type="entry name" value="AMMECR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002733">
        <property type="entry name" value="AMMECR1_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00296">
        <property type="entry name" value="TIGR00296 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016:SF0">
        <property type="entry name" value="AMME SYNDROME CANDIDATE GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016">
        <property type="entry name" value="AMMECR1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01871">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51112">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMMECR1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8153343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="190" mass="21704" checksum="0EB3B526C199ED99" modified="2022-10-12" version="1">MQINQALPIHCAYCFDVLIASLQKKELPKPTFQEFDVPVFVTFYANKEDLRGCIGTFSSGPLSQQLAKYTYLSAFKDSRFPPIQTKELNSLDVGVSLLVNFQKGKKWNQWEVGKHGIIIDFQEGGREYGATFLPEVAAEEGWDINTTLEHLIAKAGYRKNYQNILDKIQLTTYETSKAKMTYAEYLELKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TNK6</accession>
    <name>A0A8S1TNK6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0240204</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TNK6"/>
    <dbReference type="OrthoDB" id="292103at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8153418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="78" mass="9079" checksum="C61B8C06209BB7CF" modified="2022-10-12" version="1">MSKPGIGLNSLHIVFIQKSFGDIDEGDYTRETKEFSEKTIIKNKVRFADDILICFFHQQDKVTQINEGKKKTIKEFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TS94</accession>
    <name>A0A8S1TS94_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0260218</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8154913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TS94"/>
    <dbReference type="OrthoDB" id="1405469at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000097">
        <property type="entry name" value="Probable serine/threonine-protein kinase nek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="6"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="476"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8154913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="511" mass="59137" checksum="0E62D124075AC58E" modified="2022-10-12" version="1">MSLKDFQILQELGEGAYSKVYKVRRIADGFEYALKKVKLQSLSDKEKQNALNEVRILASVRHANVIQYKEAFLEESTQSLCIVMEYADDGDLFQKIVECQKKGMLMAEKDIWNYFTQIVKGLKALHDMKIYHRDLKSANVFMNGDGTVKLGDMNVSKVAKKILLYTQTGTPYYASPEVWKDQPYDSKSDIWSLGCVLYEMTTLKPPFRAEDMNGLYKKVVRGYYPKIPTIYSQDLSNVIRALLQVQPHLRPSCDKILQLQAIINRMDDKVLIEEEGAKFLLQTIRVPRVMHYLTDRLPKPNYAPIKMMRIDKNQFIQTLAVQKSSQENVEDPLTNSNIEHLPRIGRRLDDSQIQSYDAQSVISKKQNKVDESYMLPKPSLEIASQGSKNSEKLNLIHQIYAGNPVKAVKKTKKPIKPEIQILQQKLVQKKLDIEGEPKPLLIKNVKHQQEEKSPSPFLPVLPAISKEKRIMKDNNEDEQLKRIKQTEEQIQLLKEKQLKQKRKYQLRNSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TT57</accession>
    <name>A0A8S1TT57_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CDT1 Geminin-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0250231</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8154159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TT57"/>
    <dbReference type="OrthoDB" id="296876at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8154159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="341" mass="41123" checksum="6F78094A3E906FA3" modified="2022-10-12" version="1">MSYSRNGVNQTKNKDNSQHITKRDKKEAKVKGNVQQLVQKSMTKNKSLYEDSLTINSIEQFENYKKMVKLEQKRESTIDISIILFKEPLRQRFERIQSIKTIQQLLAIPYYQLFCKMFLNLNKILINQTLAFQEIVIQFKQDYQIEFKVQYLQQFLSIWNDSFSLSWKHHKNNKLSLEVRINNQQFLQNNNKNIIFQQKLLNYIQTQGECIDLFPLPNIENTQQQDIKLQEQESQMTQIIFRDQQDYQKNNQLRSIAIQLQHYFHIRDVSNMYLINVEKYLIKQKFDFNFNNGLQTIINELILKYPEWINMIQGEKEQIIRINRSINIKQLMLEQECLDCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TV62</accession>
    <name>A0A8S1TV62_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0260221</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000026">
        <property type="protein sequence ID" value="CAD8154919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TV62"/>
    <dbReference type="OrthoDB" id="303238at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8154919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="307" mass="35974" checksum="79A089359D0DB07C" modified="2022-10-12" version="1">MEIIPVETKKRIHEFSYAQKVSNQMSPDKSHIKLNESLFKAQILEKQSCTLIRNIQKDLMMKIKKYDSLLKKSNTQKNISSSQPTSPPIWKKQEEFQEEYQEDTQLQNQIKPRGEKLLTEIVHQTIPNDIRKESFPIKKLDVKLSNFQEEKALFSSQDISPIKQEQNNNKLYLRRNTTQNTATPKQKKSSVTDFLGSYAKLHVSKSTSTKKPNLIESKNQVLTTKETKEIKQNDNKKLQSTNKKQSETTDYTFRQKKIEIPVDKQNRIKKNQKQKQSVDLCVNTDLKEIIHIINDLDLFNNRLKKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TWS8</accession>
    <name>A0A8S1TWS8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0290109</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TWS8"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8156668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="130" mass="15401" checksum="3C8DCDB4DC9CB7D2" modified="2022-10-12" version="1">MAKVHKQKGYRLENTISKKGFLELRKMLGMPQFEFMHENSNLMKIQALQLNIDDDEKWIKTYKPIPEVRLQKYLLHCQYIDKSIITSLNKVDCFLNQQGKLKPIVQNKEEEKQKKQNSLSTEVQYSNKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TWW0</accession>
    <name>A0A8S1TWW0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0270148</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000027">
        <property type="protein sequence ID" value="CAD8155529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="310318at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="899"/>
            <end position="917"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8155529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="931" mass="107921" checksum="1EC4DD57AD3BD1A3" modified="2022-10-12" version="1">MIQLFQQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKINKIELTLDNIFIVTDTFIIDLQLKDSIKATGYQLLQFGTYAFFNLLRNIVVVQEFFNNQTIISTYLINQPQIFISQDTSKLLEEMSQTVKLDIQVKSQLNNKFMQYTFQIQLIQLDVTENQNPIQFQQDIEDLILQFPQDKVIDRSQIFLGPNLTLSLQSSNSDYLYASVEGLTFEEQSIQNAIYSIACSFSRQQELYCTQFMNDTIILTIENPAYLSQRYNIQTYLKLQKCSCIKTAQNINVLMQFNSSVLIQQFDYSLQPTKQILLPFQMKIISAEILKDVLFTTTVDGKLIATDIYEKQQLFTISNYNFQEIYINPYDFPHYLFIDNIDELIILAYNGKQSYEFINSIQYPIQNYNSNAIGILSNGIYLAFCQDNNNFIYFYQKINIYLQNSLSNYFDVYFQGLEIYQNPKILSTYTSSNFYLIFVQGNFFQLCQFSGTGTSYTALRTSLLYQLNGLSFIQMSSIQDQRPGYQVQVVYLFTNKQKFIDHPYLSYDFMLAISYNEDDYIGKINLTYVAQNQNFSSFFIQNVSIIYENYLLLPRNLHDKQTYSIQNKGDIKINPRTLFYGNIQNYTVNCDQCNSFNIIQPIAFSNSQSINISVENMLNQNDILYLTDQQNNLYQFYPETNNLSMIYNLTDQGNQNCYFLFIEMQNSFPVQICTGNSTIIYAFNTSSNQIIQCNFTEVCCFYYSTYQNGMLTDFLMANFKVWIFLFFIFGFPITKLISCKFSILMEYAIIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U0M1</accession>
    <name>A0A8S1U0M1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0310219</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000031">
        <property type="protein sequence ID" value="CAD8158200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U0M1"/>
    <dbReference type="OrthoDB" id="289307at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8158200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="353" mass="41781" checksum="732F2D9BF6A090EE" modified="2022-10-12" version="1">MNMFQLLFTKFADILLSSRTYPFLQCQPSIQNQVKEIGVIWTKEMNANVKQLLQNNWRTSQQNEMIVQFFIENDGNSFLVEEWCLIQQQGTISNQLTNKNSNSLLKQLTIWLRSAIAMMTMTSIFQEELITKQYKVSHSLLFYNKNTKKRSDWIDDSKLQVKKITTKFLNQQNLQLTIAQQGSLEQVLQQRINRTESPLYKRCRYMSEQIYDKDSQVFQRKMTMDSEQLDQQKSNLIDNTSPSSANNLNRSFISVYSKKEEVELLFTPEEAVLKFQDKNFLDEDYEINMMTNDHIQKEIFISQIMITNKLSTEEKNCQQQTMSILKNVHNKSKNKRIRSLQITRLLSFYRQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UBM1</accession>
    <name>A0A8S1UBM1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0340115</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000034">
        <property type="protein sequence ID" value="CAD8160026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UBM1"/>
    <dbReference type="OrthoDB" id="302114at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="17"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="190"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8160026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="357" mass="41649" checksum="A877FE8E05B1A6A0" modified="2022-10-12" version="1">MNRTPHMDQLISKIYRQRKNQTPNDYSREMYSHPNSHQTSNKDLLNLTSKQKLNKSCNYNSNLHQRLQSKAGADIKQKLLDEILRGSHSNISTSYLISTLKEKVSEKVNQKVQKYNTCQEASSFTIPEKELSPKPMFKILENFLFAECQNLKNQVSLNSFQELLDKMEIKEQSLKISIFADLKKLFIILAQSIRNDIQTQKKEIESLLRQNNSFKSQIQEMNSQLDKFKSVIEDQQKQIKIKNEENFISIMNTLRSHGVDPRKMLKKCQDNVETCKLKQEQSEFIDESQKYSSDDSIPFVDPQTIIKNEKKVIGLALNLNPLKDPKKAPIGYQDEFMANINEFSESWRQQALAEKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UDT1</accession>
    <name>A0A8S1UDT1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Casein kinase I</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0390018</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UDT1"/>
    <dbReference type="OrthoDB" id="5979581at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001190">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="17"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="310"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="315"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8163075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="422" mass="49578" checksum="51A92662C8AD33AB" modified="2022-10-12" version="1">MDSPLKDMTGTIVNKQYKLIKKLGAGAFGEIYLSQSNGQEYAIKIEKSDSKHPQLEFESKLYHYLNNHNGQGIPKYYGYHQQDGYNFLVMELLGQSLEDIFSENNRIFTLQTVCVLGIQMLECIEFLHSKQFIHRDIKPDNFLMGKSQKDRVYIVDYGLAKRYITKDLHIPYKDNKALTGTARYASINTHLGIEQSRRDDLESLAYVLMYFLRGSLPWQNLRANNQKEKYDRIMEKKLATSSETLCKNYPQQLLQFVDYTKNLKFDEKPDYQFIKNLFIQIMQENELRMEYIYDWDDEDTQRDKIQIRNEEKTNLLKTNKQSSNTQQRINKHQENKNINSNSIYMRNCSNINNKYSIGRTKTNSIGSINKIIVNKTKQITNNTINSLTNQNTLPNNNNNNNNKLHQNLAKQIIKKHSSLHYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UH02</accession>
    <name>A0A8S1UH02_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0390319</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UH02"/>
    <dbReference type="OrthoDB" id="316464at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="60"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8163634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="327" mass="38222" checksum="E1AF4DD3E2766E19" modified="2022-10-12" version="1">MIGEKIQQISQFVEITISQAFHQISQERLVQCNFANEIVAEITPLIDQIVYLNKEKQQKFELAQITNVELQKILQELKERKKNDVNNYDQLYQESHALLQQKEQEIIQLNDTQNQIIKNQQETIKELLKQKDDFMLENNKLNELIKQQQENIIQDQQLQTPILLNSFTFSEIYKQPSCQVSQNGKLFFNKSLFYSCLCDQAIPKQGNIVTNQTMVGIGAKEIISKSGYSNIHLPGNGQRQFIFSAYLIMSSGYCFSHHQQDKYHDSIPFTFTNNDIIIIEVDIQKQYIKWIKQSTKEFFLLDIDPNYEYYPCINGTGKVEILNEIIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UHA3</accession>
    <name>A0A8S1UHA3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0400036</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000040">
        <property type="protein sequence ID" value="CAD8163734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UHA3"/>
    <dbReference type="OrthoDB" id="299218at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="48"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="260"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8163734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="367" mass="43940" checksum="176A778E2EF22681" modified="2022-10-12" version="1">MNFEEDNRRKEISIYDKQCKVWYEFLKEQANKINNYLNDTKSGLDSYKLNLEERLKELDEQYNSHYEILDSLQEQISTIEYYQEVQEQEIEAYVNAINSAQDEKDKHSRDVLQKEKEELITLICEMKHSKIEDWLKTIKKDLPDGDLKDILKTCYRLFYGAKTFSLEEFIENVLSKGESKTEIEAPANWIKEKGLQQQGNDKLKFKLSSFLAKFAIYNRQELNKDSRQMLKQLLDSQVQRQKQTVEVKFLVSLLEKFNNWDNASSENDKLEQNIKELEQTLEEKQNSPEYVQQQNHLHFLQKEQEFTNEMIEKITNVRSSLEDLLQSFTKYEEYQQQQVYDMQSTVEFNSRNEEIEALQTKEQIEQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UJJ0</accession>
    <name>A0A8S1UJJ0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0410127</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UJJ0"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8164625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="52" mass="6593" checksum="D98B7914839E6C5C" modified="2022-10-12" version="1">MFYFNTHNIWMSEISHIFKEKFNSINQNKLRIVFFYFITRLFQIIKEIQRNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UK13</accession>
    <name>A0A8S1UK13_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0410221</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UK13"/>
    <dbReference type="OrthoDB" id="312923at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="282"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8164805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="296" mass="34159" checksum="CB93D5ACA344B441" modified="2022-10-12" version="1">MKKQTKGEPDLLIESMQKLIDHMNDSKQHQEEIFQSLTPTDVQKILTNFEDKNQIMRKTSAAFLCELVFDNPTVQKGFCEISNILPMDGKICVNKIPQSLLSQSKNLPEIFETIKNAQIPFDNDLNYPLCWYFENQRDKVWKKGVKFIKFEKSNNGAKNRIEKFIDPQLHLFGFIIANKKDIQSQDEIKKATIQTRQQQSVSTYESNVKRIPNVQKMRPNSGQSPIQSVSPLIKRQRVMNASVDYDTSGTKSSLGKTIIETEQLQTINEIDRPKSKLETKKSTTNNNISNIQKRFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UMD7</accession>
    <name>A0A8S1UMD7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">USP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0410280</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000041">
        <property type="protein sequence ID" value="CAD8164899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="289038at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000022">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF827">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP" evidence="2">
        <location>
            <begin position="1417"/>
            <end position="1747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2352"/>
            <end position="2372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2359"/>
            <end position="2372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8164899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="2372" mass="281757" checksum="3BF3BC11FEF6038E" modified="2022-10-12" version="1">MDIKKTKKKPELSEQLFVGNYVDVYHQESKKFKLAYILQKTDKEIEVTYDGLSKKENEIIKLSQNRVQFARRSTANYTGDDQKQQKTSRDYLKYSREDCEKYTKELITIMQTNFQGFTPIEIIQCVRVRQFIYLDMVLSNEIQPKELPIALEYIKTYYNFIKWYFDQFPKYFADYMKHLNNNELYIIDERVSIASCLQEVSEAFCMLFGSIKRLLKQQNSFFYLNYDHLQQLIDKTFMVPVYQGFVFNPNIEDWKLYPEASDVIKCIKKSWQFYLKTMSYFRSIGGLQSWENLLKPYQGGEYNYIPLKAMQKIVLTQQYLSQYFPQQEQSNMAKKTLEWFQQRVANLTIQDIKDTDIHQIKEIVQEMQYYFLKGYSQDNLNNLVDEIQLQLALTFLKSNFLEKRVKGLCEIKDFTEKFKFETNSQIEMMKNSMNKDEFIQLLIQNKILDYTLLGDSAHPELIKRSSDVAIFLCKNQVFQNDYVDKIWLNNYNKHETTQLALYEFFKAISPVLQFQGIENLYNHILKIPYTKYNENIVSMIGTFTKAALSQKFHEQQLQLKTDKRFMTFNQLWELLQDRENPQVDSNIQEQCFQAARTIISQIPQTKQFISYYFEKCFELIGNHKSVYQAISFVHYFLDKQLKDDKGKKELIQFTDDKYNIIELFVKDVEVYMEKIRQYFKNEEPQDIIIYGVQKFSQNVLFRLQMLNYLLQQTDLKINYQQSFRLWNALSAKTKGGIQKKELNKIFISDYQPNISSCKINQVYFDNEGESKFFTQVLCNPERNDYENYTIEDFKLFQVFFKSYNQSRQMLNYFVNSIRFIVNNHNFEGKNAIWQIFAKVKDLGLLDQIANFIINLYTKLSQTLDIKCDKIYQEMMDKCLELISQQCPNLTNRSINLLLSLFNYFQSGPLLKKSIKPNSYTQFQVQIINTNNQITIKEFIEGDTTTINQMKQKIAEDLQVAYQQLDIIVDNKPIEQQVDIVETQVSQVIQHMAQVKVRINNKSHPKNYLSQEQHTFDILLKLLNKQENTEFLSQVWDLINSLPTNIQIKKQVENCKDWKKYLDHQFFEMFYVLQIIQALQENGQWCEQFNNSDGVNLITQKFLGQHLQFQQRPLEIKSCLTYLDILSHSNIKIKEPQIIDQIKQKILEIIQELSYYIKTKKKLELGQKKTSQQKEMNQIETRLLRGCFNYLHKFGCKSYIRQNHDLQQQIYNYYVEHENQELKKEYSIQLLNFKNTQESKLLIKILFDDVLKKVIKSNKQKCKRFFEFCCNLLQQENNLNIENFDLEDLLSYIKLKLQQLPKNEITIKDQDQILIGLLKLLNAIIDKLPHLSNNNQLFEQILSYLLENEGETRCKCKSQQSRTAAFNCLFTLLKNQQNMNKFLNNISHLHSTNTWRTKNLNDWNIQSKFKEKSSTGYVGLYNLSGISYMNSLLQQLYMVPAFREKLLQVEDKNTCVQEENLLHQLKCLFLALKYSQKQYHNPKKFCYAFKDLDGNPTNIFQQMDVDEFFNLLMDRIELNIKSTSDEDLVKRFFGGVMSNEIICKTCPHNSEREEPFFAISLPVVNKKNLGECLQTLVHGDLLEGENAYNCEQCNKKVSALKRMCIKKLPDHLILVLKRFYFDFDLMAKAKINDRIEFPFELDMMPYSQQGLSQQENRINNSKGQDNPQEYYQYRLTGVVIHMGSADSKHYYSIIQDKDDLNKWYEFNDILVTSANIKDLKNDAFGGADKMLKNKDQSKNAYMLFYERIYPIYIADQLQQINIELDQKTVQFLDEIKDENRKFQIQRFIFSPEYFSFMQNLIKFQQQQQFVQDQIIQKLVFFYLTCAVRENEKTFITNNILDIQELLRKSPNTSQWLLKCFTQQQYIREFQFDCSKKMVRKFVIQMIITAIETVQQQEGYQNLFEIVDDKPKSLVASLINVWIQMITELKKSLKNSVEFYDIFYKFAKLNKFNAQYLISKRLVGKLLDLAIDVLKVNTNIAQREVVKKMDDVRIIKINDDPNNYLGQQNNQNFDVASSYYDELLEEKFEESINSGPSTSRVYLWRVIAYLIKGEFKQLLSAEEQILLQFDLQFILQLLEEGDCKLAIRMISDILCVISMDNQKQSDLIINSIIKLINDREYKDYRKYIVVLKRLFQMKDQLQITRINIGMTKLLDIMQKQGYYFLETDVCQQYILRMVSKNQVIHQWMVKNQRLWQWILEMNNSQPPPNDKLISNNQIAQKCLHRLHNIYLPNSSQGYAKLLSWKKQQYLNLAKEPFKLNDDFDTDDDLTEKNVKVDEKIDYYDQKQWITATISKVMGDYIHLTFSSKMAPQNMDIELDNERLAPFNTLSNNNKIPGYYNQSYDLVIEHQFDHDNNDIEEGNNNSNNQTDSDGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1US50</accession>
    <name>A0A8S1US50_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0470039</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000047">
        <property type="protein sequence ID" value="CAD8167284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1US50"/>
    <dbReference type="OrthoDB" id="346907at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="9"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8167284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="465" mass="54945" checksum="8E16AF63E5E4B80E" modified="2022-10-12" version="1">MSKHPIQNYQILKTLGKGTFATVYQAQHIKTKEIVAIKQIDLDIKEGIYKLKLQKLLETEINIMKSIKHKHIVELKDIQQNKDSINMILEYCSQGDLQMFIKTNYPSKTLPESETKQIVLQLLDAMKYLRLKNIVHRDLKLANILINEQIQFKLADFGYAKFLINDELLQTYCGTPTTMAPEILKNSNSYNHKCDIWSLGVILYRILYGHPPFVSSKGTAQDLLLTIQNNNLQFPQNIFVSAECKDLLKKMLVVDPKNRINFEDLFKHQWCYQDNIDQEKSLLKVILPQHQDFLKNLNYSINQYQQQLTQIAQSISSIENKYQDLKQFCQRFKNALDKAFSTFYLKIYYSNQNVVIKVPQNYKYFNYKQQLLVFLINIYEKALQNNINIQEFVNLNIFQFQICNQLEVRYEDYSITLLDEIQNFKQMNVQSCQMLQVLDNQENNNKILNSDLQILTEYIIQRYKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UXS1</accession>
    <name>A0A8S1UXS1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0490248</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000049">
        <property type="protein sequence ID" value="CAD8168702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UXS1"/>
    <dbReference type="OrthoDB" id="291577at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990604">
        <property type="term" value="C:IRE1-TRAF2-ASK1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036498">
        <property type="term" value="P:IRE1-mediated unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045133">
        <property type="entry name" value="IRE1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13954">
        <property type="entry name" value="IRE1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13954:SF6">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="215"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8168702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="463" mass="55244" checksum="6CFA922C00554EF0" modified="2022-10-12" version="1">MQNKYKKVQDIYIEMDKKCLLEQNKYETRLGLCEIDGHKKHAVIKIIGNDNQPESLRQIEAIRNMAAKTQMFKGNPYIIECYSRQRTQNTFYFAFKKCKQNIMQVTLDFYEILHMLASMIQLLKLFQQKGEFHGNLKPTNIFQQKKLNSTICFSTTDFFPYQYSNRILDEYIDPEFDKDRPSIAHDIYSLGKVCNFVIKNRKCPYQNLINQMNCSKQDRIKLDQLELQVEQMIEKYEQKSLRMELNQKGITEEVIQKIIMQQVENQKKKQLEQTKWSDFELNQNVDRDFNIRDRAQFLYLMSQEIRNLQSIDQFLLDCLILSCLSESFNTFQECQKITQNKKLKQLVESDLIQMNKYLNIINLQCEIYIQDLILKEYDVGFFKQYISIYSQPNHLFKKALSLVMEDIINKNLTKELLLNDELDKSDIKALNYILVSNQMKQDVCYDYKGLQQYIQSQLQVIQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V244</accession>
    <name>A0A8S1V244_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trichocyst matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0550015</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V244"/>
    <dbReference type="OrthoDB" id="303944at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035778752" description="Trichocyst matrix protein" evidence="2">
        <location>
            <begin position="16"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8171420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="357" mass="41157" checksum="581A731BC3F45DE4" modified="2022-10-12" version="1">MKIIVLIVLIGAALARDEYFFAQLRDSEFGKTIIQTIQVQLLQEHPADTVVHLLKQMKDDLLNEQRAEDEDVQVALQSCQTASEQAAAVITVAKERKATSEDRLPLLQQEQNDKKQQLLDKQGEEQRNLDRISVLQEARNVQRSEYEQRRDELVGLISALQEAKQILSQGITSLKKTSFAELKSHHQNFLKYYPHKKGFHTMVNMLLEVLQDEGTQESAAQKVVKIIDTLVDSIFQVQKEEMKADDGRELDFQTQKERLLLANRRLEGSIAELNARYLVLGQKILEVKNDVATQDSVIQNKSTEKGDWDQTCNDTEKAHRQQTDSRNSQLEIIVECIDIFETRFDMETKSYIQRIRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V3G9</accession>
    <name>A0A8S1V3G9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0550052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V3G9"/>
    <dbReference type="OrthoDB" id="311001at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8171491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="272" mass="30506" checksum="9D6080039977B244" modified="2022-10-12" version="1">MAQQSAEFATKVDESHKGQDDEKNKPQEVNQQNEEKNEAQKTENKVPSLFGSMINGQNLFNNNQTNQIFQGSLFGSTQPNTGIFSSGPSILNNVNVDLTKPGPSLFGISYTAPKSDDSDPGEDDGQDDQKPEDDEPKQIVMKYDYTSNTDQLVKVNVEKFRKNTNDILEKGSVAIEKTKNGNSYYLVYRNAIQQSLYTGQFIKGFSEIKPLGQKAENLIIKAISRKDKLDSKQKEQGENDEKNSISVDTLKVMFTTKEGSEEFKTEFAKIFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V5H7</accession>
    <name>A0A8S1V5H7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0550138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1297"/>
            <end position="1322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1411"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1596"/>
            <end position="1622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8171663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1668" mass="199915" checksum="232B39C37EABEB4A" modified="2022-10-12" version="1">MKKFCLYLFRVQNLKKNSRTKAYCLVGIGLEMEYREQENNIKRQGNLSDYLQILTYFDPEKLCQQSSIILITILIIGFCFPFSYFCLGVISSKEVKNEKIKSSSLKLYSSCIDLYYWCLFQPFIQVQLEVLLFNHQILDWNQMLKQNYTNVALIFFLSLFGLFLNTLMMTSILLFFYQNVNLKIDFLSCSNKQIMLDIFIFKILIAILNSIHIQSIPHYVSYLIILILLTLKLLENQFLGNDRPSFQYIQLNQFYQHLMFSLLGVLITFLIELSVLQLNQEINDFLCIIIICVFLNVLGSIFQNKIVYFYLMQNGISGQLILNKIYYLYQLNTSSVDKKYIIIGLIEKHLKKGCISKLEKKQQTLEQQRDPSFYNSSIGYCFCQQNQFYDARRKIFVNSQTSKVQYHYKSFFRFEIKSLYEQCIIQNKDNYQIRLLYAKYLFFSIQNKSQALLQMSILKQKFNKLSFFEKIDFHLLLLMMEEKINIQNSNSYQNYLDFEYVIDIEMIFKKIKMKIADLIQNYIKYWNTMDEEKIIESDLLKLDNKIQSGIYECEKMWLNLNNYNKNYDNSQNDINYISKKPQWELLYIWYKRYILNQKMKGQVIDYPVISNNQQNFCDEDSDSDYENHYMQAFDPKKLFHSMTAIIFAKENGTIVKFNEYTRQIFGLQGFININQIIPNSMIRNHMLRVKEFIEKGKTSSLYARKKVLIQYQNSYLIPANKYLKISINQFMLLEFIVMIRPIQHESSGNYLVINEDWEIVQTTKQLKNIFEQQFNLLLSCPKLLLYSKYKNLLENEDYKLFQIKSQKKQSLQKQESLPGVLQRQKSLLNDNVFEEVNQEKQFTEQFIKENEDDLDERQRLLNSNLQNKNEQYFQILIRIPILLKRMQEEYFNLIINAEKENFNDYPEDSIMKKQSIIIRNGRKKIALNFKILIEKIKFLKRIRLMSQLEAYRYLYRKYFCKADQLKKVIRVDAKIKVEKNLLIDKIQIIKFNKFETFNNIKSKRQSLLVKANAKRKILQIKSEQKLQQCQNEISIDYTTNNDIKTTKNENQVIYPMKYLMDESQMKNDFSESQIQDISLKPNQNLFIKEDHNKINKNLNKINYFKWTNRYFIIGMTLLTMLSFSYGPNTSYNDLYLLKANSSYTTALISQDFSSIYNQLIDMALCNQNVTLCSNIIQYTTIQNKQDLLEQNIQLDLQNVNQSFIQFYSSKFNIKTISSMILLPHDQEVHKQNILEDMKNFQVLLQQFIITNLQEITFEFCYYQILQQDLMPGLYESLSQVQIDLLQQIYLEFDFRSLFFLVNMLTGLIVLCILIAINCFNLFKIIDHYQTMYSQIQYFDQSTIESVIKYYKQLRQYFNYFNELQTYQTNNYISFNSEGLIKVEVDYKMTMALKTTKFITSDYWFKLKLRSILLYSIFIFLLILMPVTYNIYMQKYANDLKLEISQNPFLFETTSQWVFTFSKELYIESFYDIHNIISHEYQEYLEYFIVEALNTDSNSLQSRVLSINDYFYSDICQLLNSQNITYCQNIADGILKQGLQQTYNLMANLFESQLDESKTKFTEVSFDSIYNLNLLQPYIFASRKEMWKIWINNFQDIFSYFVITETQLIICFYIFSFLIYFITLELYHEQQLRLDYQKARNYYKRYFPNNILNEQKRIKAVFKKLSLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VBV0</accession>
    <name>A0A8S1VBV0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0620143</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000062">
        <property type="protein sequence ID" value="CAD8175048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VBV0"/>
    <dbReference type="OrthoDB" id="287608at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8175048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="189" mass="22402" checksum="0040D9846C6A44B9" modified="2022-10-12" version="1">MQFIHDYAIIGEEENEPKLINPQPLNQHFQNFSNLLQAQKERQKSKIFMPLDLNMINQSQNGYKSSARLSPRSYFTNKQVPSVDKIMQKMAIKHVSHHQVPTKKEEFYERQIRWLNQIKQTNKVKNQHKLNQEIQNCPFKPYTSPQQNINKEKLKENSKSILSPQPFKKPNKRNNSYTKIHLAKKQSFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VF60</accession>
    <name>A0A8S1VF60_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0610090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000061">
        <property type="protein sequence ID" value="CAD8174442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="285065at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002895">
        <property type="entry name" value="Paramecium_SA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01508">
        <property type="entry name" value="Paramecium_SA"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00639">
        <property type="entry name" value="PSA"/>
        <property type="match status" value="28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035889734" evidence="1">
        <location>
            <begin position="17"/>
            <end position="3198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8174442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="3198" mass="353336" checksum="728FA0185F203F6E" modified="2022-10-12" version="1">MTLLAFLVIEISFGFSVLKMDQCSECSKLIRKQDCEYGNCIWSTNNESIEGICIDNGSNVDQGNSSGQADYCKSILNPAQNCSKVKGCAYFNSSCTFFTGCSAYFFHTTFDCQQISLYCISEGDGCIDAKKCEEYLTQDICENSGSSSGSGHCKWDSGNLKCRDELCSEAGIGLTTDQECSKFRFGCITKGQGCTESPLKECKTYESSGQDCSKLIGLDGPCEFLQGSQFCQQKKCETAPITHTKDKQCDQYLKGCVSTGRGCVEKLWPCNTYNKDCTSYVGSDGICENEEKSENCRSRICENGLFNSDEDCNQYKIGCVTNGKSCVTTLQNCSSYKGNKNTCLGFIGLEGMCKGIDDNEQSCSVMDCVEDSDSKYVTDEQCQKIQKICKTNGMGCVYNLKLCNEYDGSAETCFQMIGSDGKCKGGTDGKCQRRSCQDAPSTYNTDYECKNFQSGCVTNSNGCINQTSCQLTLKEVSCLGTAGCQWNSSCVDSTKCSNFNTQVICENNSAINYSSADGITTYYYTKCSWKNNYCQNLSCSDLNGDKYDSDSACQQVMSNCVYSGYGCVDRQSDCSLFKGSQNRCALYANKCWNDQYATETSPCRSRLCSDNIIFTTDEECAYFQKGCRTNGKGCIEDTKLCSDYQGTQAQCNKFTGFISNSSDRQQCYNTIGATITTNCIVKTCDMAVGLTNNTDCTKFLKGCIWNGLSSCVKETSDCTAFYGTQQLCNTFIGNKQKCYGLNTNNAYSQCKIKQCVDNNTPGLTDKQCNDFLEGCVSNGIGCTPKETTCDQFSGTSDTCSKFLGNGQKCTRMSNCTTRLCSDYTNPTKHEDCYNYHSKCRFVNTQTSCIDQTICNNYVAQGNTNTEKTAYCRALKDDSNFICSYQTGDINCSNYICEQIMTKLECESYTSTKLCYYINNMCVSTKDCSTITIDNTSANKPTTNSTKLTWCNQYKDINNKQCSYHSTLSSSKCSNFILCEQVVSATNSADCNRLLYQDPDTKNKGCLYYSNQCYTLKPLCSDYFAIGNNNTEKSAFCQAMKLNTSIGTDNTKVIQCQFVNGDSNCSSATCESVSVANNQIDCDKQALNCVYFQSKCYTKQNACTSYSTSSVSSNKPIFCSYMKNSSGEHCGYIDGMNNCINSSSDCQLYRWASYNSNPAPSPIPSTNSSKRTYCQQRRSSSGRLCGYIYGDSYCTLETNYCELIQSPSSQLSCDLQAIGCTYIQSVSTCATTSRLTTCNDITFDDQILTTDADKLNYCKQVKNYYGYCTWMSASGYCDDLTSACTSINISSISNDDDKKTYCLSRMSKAGSCAWRKGDTSSACRSFSCFDIMNPVNQATCDSVMSGCTYYQKQCITSQANCNTYYADGEDDDKKLYYCSGLSQTTSNLQCTYIKGSSFCTVKTTTCASYTVSALSNADKLTWCYLLKDLSSNICAYSSGDSACKQITCELIISPQSQNDCNSYLSGCQFYKGYCYTKPTNCGSYSIPSTITDNVSFCTNFKNTSNQYCGYVSGSTCAAQSSCTVITAVGVTTTLKQQYCQARKSQDGYVCTYISGTYCSQVDASLSCTYITGTITNQDSCQSISANCSYLDSTKTCDLNSNLNTCDKYLFSSTIDEDTKLSYCRSVLSGTCNYQFGDTKCSNQVTSCGQFLITSVNDKKSYCAARNSSNGNCAYDEQNESYCSQIKCSDIELPNSQMDCNKRMSGCTYYRNSCVQIQNNCNLYITYGSDDQTKKNYCEGLKSSDNTLCTFIPGVGVCFNQSSNCSSYDVSALTDKIVWCEQLIQSDGQICTNINDASSCSTKIYTCETITANSQSDCDKILENECIYYNTKCYTKQSSCSSYIIPLSITNKQLFCKNMFDSSQKYCIGGSTNCGLPTTDCTNIVANGSDDSQKRIYCRDFKSSDGQMCGYSSGLNCEIEHYYCSVFISATSQEYCDQRIDNCVYIQKSGKCIEKYLVSDCPTMLFDSGLSNANRISYCQSYNPYGNICNWSTATDHCELDTKQCIDFDATGQSDKRTFCQSKQGYNCSWRNGDTSSLCRKFKCQDVDLAQSQSDCDSKIFGCSFYINSCFTIENNCDNYYALGTDNQLKQYFCNGIPTISNQKCTYLSDDYKCRVRDQCTTYNVQNVSDKVNACLGMYNPTGIQCIYVKGTTCNQVDKCETYTGLSNMSDCTILFDTNKRNCIQGNTSCLTFTCDNISNPTSQSDCDTQLKGLCFFNSTNSKCINISDCSTYKKSDWPVNADPIQHCSTLIDKNGNNCTADDATSILCRERLCSDKLFYLNSDCKEWKSSCKSDGAKCIESIKTCSQFTGNQDSCSKYLDSDNLNFCKVINGSEQDVGPCLSLSCYQNTTANSDSECQSYQNGCLTRGVGCIPNTAKCDEYRGTRDQCAKFTGYVSSTITEICSGDFTNTDQSKCRSRICSDNLIDNSDALCSYFKTGCVTNGQGCIDINASCSSYKGTQQQCSKFMGNNKTVYCWNNIDAPSNYNCIDKLCNHVLGTTDQQCEQAFPKVLINGNLTLLCISNGINCIKNPAKCSDFIGNNISCQTFTALTDGPCKGNLDNSMGYCQPRVCYEASKSYNTDAQCQDYHPTCLTTGQGCMKFSSYDNQGKPTLNCNSIVSNDVCSLKLGCTLASQCLVSVSSCSLVKSQAICQTTILQNGKNCVYDLSLNQCREFQCYDYVGINDNNICQQFSNNCTTNGNGCEIMKSCVQYFEKLTCQSAYSTDPINRCIWTDQGCRQRECKDLKGTTNQSCKQFISGCITNGQNCVGPNYLCQDLLEKTCLTDYQGNPCIFYKQTCYSYSKCEDMKFTTHDECQQFSELCTSNELNCIPLNKCQYYTKQVSCNIGIDGMCGWNNNKCNLFKQCSDVMGTQTPICQQYSSICVSDGVKCVEQNICSFYNSQFLCEHNKGLDGVCIWIDNACRLKQCEDLTTNTDTFNKCFNQLSPMHCSSNGTKCISLSSCQYYNEKSCIIGTDGPCIYKLPLDKKSGQISCRLKQCQDVVGKTLDICLSAFDSSYKKCVSNGSICVDFNNCSSYTTKIACTSGGLDGQCAFTPIASQSNDGICKLFTQCSDANSDKDTCLSNSKYCQWISSGVTKQCIPHTCLTYNSKKDCLPVPSYDQKSFLLCAKINNICQEVQPTTLTMETCYINSAQTYIWNTSTSKCVQCKIKTDNNVAPNSQVSTKSSVLLFTLAIIFNLYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VF92</accession>
    <name>A0A8S1VF92_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Palmitoyltransferase</fullName>
            <ecNumber evidence="10">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0630131</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="10"/>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VF92"/>
    <dbReference type="OrthoDB" id="331948at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF301">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="303"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="340"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="12">
        <location>
            <begin position="256"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8175587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="424" mass="50124" checksum="306A07B1E4509B77" modified="2022-10-12" version="1">MKIAPILYLILQGSTLLIFSILNPQFFDFSTISFYYFAFVMILSLIFYGLTSFKDPGYLIIRSPNNTDCSNQMTQKRKIFQFSTLPSNARTYPIIEQQNPIQEQKSENEQELPCEASLDINQLPEFDMNQIPSFTKTPDKYPSPNKQNNQQKIFQDFDTKQASTQAFSITSQNEHETIVPITNNDKQKDVDIEKQEENVCQKNKNEEKIMNKNLQPSLTIQVHSVQNEFISEQYSPGMSERNKITQSTRAVNKILIEKRYCPICSIDQLARAKHCQSCKKCVACYDHHCPWAGNCIGERNRCVYYWFLVFQIQEIYYAFISTLQNLEFDNYNHLLKFFSIYFFTIIIFFGVLVTRLLCFQTFLSFQNLTTWEFYSWNKISYLQELKRKNGSPFSYGWINNIQIYCRFKIPQLTIWEYNPERVYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VFZ8</accession>
    <name>A0A8S1VFZ8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0630117</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lin-54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VFZ8"/>
    <dbReference type="OrthoDB" id="303998at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005172">
        <property type="entry name" value="CRC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028307">
        <property type="entry name" value="Lin-54_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446:SF34">
        <property type="entry name" value="PROTEIN LIN-54 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446">
        <property type="entry name" value="TESMIN/TSO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03638">
        <property type="entry name" value="TCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51634">
        <property type="entry name" value="CRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRC" evidence="5">
        <location>
            <begin position="188"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="347"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8175561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="386" mass="44946" checksum="9F1120ABDBA4AE6D" modified="2022-10-12" version="1">MSADSEEQNRDVSNDPQPKPDNKKVQDFTEDWGYQGNPSPRFTPMPYQQLFPPMCPPPFELEKQVYHDYDPQKYPQMNSDRKGPSEVPHMDHIDDDPHRIPMHYPFGYYPPMKYQPQPMFMKYPPPPQQGQAYMVPPNPWYHPRPQQQLMPPFCYYPQSQYDGLQCQNTVSKELQSKVLQLVQYQKVSDFQCNCKKSKCLKLYCECFANNWVCSQSCNCQDCKNRIDNPQERSKAIEEALLRNPDAFAQCFQQKSQTQFSVQQQDKPLKEPTKENSNITRKGCNCKKSGCKKKYCECYSQNLKCSDLCKCEQCLNRTDAEIQAQQDIAQVLNSQDQVNVDIKVNQKSKKIKKIGNGNEDKSDSQKPVVQLKINVNNNSSRKKQDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VGU3</accession>
    <name>A0A8S1VGU3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HTH cro/C1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0640223</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000064">
        <property type="protein sequence ID" value="CAD8176207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VGU3"/>
    <dbReference type="OrthoDB" id="10258327at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006939">
        <property type="entry name" value="SNF5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10019">
        <property type="entry name" value="SNF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04855">
        <property type="entry name" value="SNF5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="6">
        <location>
            <begin position="11"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8176207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="386" mass="45765" checksum="DB3BCB67B97E5C87" modified="2022-10-12" version="1">MQIQKDLKSRIQEILQKHNIKQVDLAREIGIHHSTLSLWMQNKNKPRKQDISNSQSTRIEELLERWIATFHANKGQGMGTGQFRLPDSRASQFLSHHTVRPVLPISKFHILRNQYQNSIKQQRQEIILRKTQEQLKNNPNISFQQALNQSAQDIPIEPPKHQQLVPITIDFELEGKKIYQTFCWNLNESHMIPETFARIITEENQLPSVIEQEITTQIKKQVQNYKCYYPTKNEMLKNLYVDLRVENIHFKDQLEWDMNNTMNSPEALAELTAKEMGMPEQYEPKIAHALRESISSLERQMEPKTGEDFSNLKKSTRTLIESNANFNQFKNVMFSNQINDKNWVRCELEYDHQFQWGPSIELLDQNALRNVQKQEDRKTRYVRRQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VJU2</accession>
    <name>A0A8S1VJU2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0630225</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000063">
        <property type="protein sequence ID" value="CAD8175722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VJU2"/>
    <dbReference type="OrthoDB" id="310033at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="121"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8175722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="191" mass="22506" checksum="3A4ACF1D94D10467" modified="2022-10-12" version="1">MTLIEMNQPRKDIKMREQAKQINSNLYTDFLDYSNIELTQNPQNAFVLIPKSYALNEEKKYQEAIQQCDKNLIEEPQHLHALYRKRYHILCTTGFTLQDLNQHQQSIQCIEKASNSDLKYSIGYMMQGYSLQSLGKQKDAIDCYDKAIQLDPNYASAYFNKGYLLTQLKKYQQAKDIYEQTVLYCKLEQNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VKI6</accession>
    <name>A0A8S1VKI6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WH1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0680059</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000068">
        <property type="protein sequence ID" value="CAD8177740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VKI6"/>
    <dbReference type="OrthoDB" id="8963340at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000721">
        <property type="entry name" value="Basic proline-rich protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23330">
        <property type="entry name" value="P300 TRANSCRIPTIONAL COFACTOR JMY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23330:SF9">
        <property type="entry name" value="PROLINE-RICH PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="255"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="337"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8177740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="443" mass="50315" checksum="F55F0FF059FEBD4D" modified="2022-10-12" version="1">MLQSLQNPKVRKIIEDVIGGCDQQTATVLDMSFVHLYTCEEGKHKFHHCRVEGILVFLIDRREGKAFYLRILHPASLEVRFSVELYFGFIDSWAPVRGFTNFYAFPFSKGFVGMLFINEQEAQRVTQKIKQYAPTKKDLEKLNLQIKEEEKLLKAYQQQEKEKDTFSYQFKAFFGKEKVEKPIQIGTPQNVKRNTLSLNIETGEIDIDKLPPVLIEVFLNAGITKKDLQDKAKAKEIFKTVARFSERSQLNTPIQSNQQNKNQTKPQQQQVTNQPSQKSNPVQPQQQQNKPQTISTNKGAPPPPPPPPPPPPPPPPPPPPPPPPPIRQGGNKPVVSQPPSQTQQQKPQSQAQNQLTNPPTQTQQKPQPHSILKSQADQKNLAKEIENSTGYKVQGHDIKMDIGNMNAKEKEDLSIMLEVNVKNRRITQFAKRKSSDESGWSDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VM00</accession>
    <name>A0A8S1VM00_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0680178</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000068">
        <property type="protein sequence ID" value="CAD8177927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VM00"/>
    <dbReference type="OrthoDB" id="284854at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF263">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8177927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="581" mass="67324" checksum="E166131E8464FD5C" modified="2022-10-12" version="1">MQYGLQCRKHPGNQVTFVCTKVNCNYGNLLCQQCINGNLQHKNEHKNYLNQLNEFIFQQNGKTKTVLIPNINNTLLELDKRLKEYNAMIDQETQEIVSDIAQLFKIFYESSDRAKKQIQEIIRKDLIEQQARNINFKQQLQEIQSNEQKRNDNNNYLFNTIFSRDQDKELPISEQSLMQIINKNGDLNQLRCDILNCLNNVVSTLQPTSNRIRYKYTQNHQQQFNLLKNDFIQSLNCLFQKLTDLTQDFHTVEDMKRSLYQFGSLDGSQSLKQSQVVQRTLGSSQFISTGNQSQRFYRSNSQSQILSVKTLELFPHLSPTIQSILNKIGHLSPPQADSLLSNIETVPPCQFPDGIIYEGQIQNNQRFGWGRAIIGQDYYEGYWRGGLPFGFGRIIMASGDYYEGFSQNGQANGRGLFCSGGYSYEGDWEEDLKQGKGQEILQGKYEYKGDFKNNVKCGQGRLIDLVTGNEYQGEFQNNQLQGEATIEYKNGDKYYGQVITVDGQIKRNGQGIYLWLDSRKYEGQYENDLEHGDGVFMWPDQWIYKGQWQYGMQHGKGIQYNPQGKEREGIWNNGQFNKWIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VRA9</accession>
    <name>A0A8S1VRA9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 4E</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0720071</name>
        <name evidence="3" type="ORF">PPENT_87.1.T0720072</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000072">
        <property type="protein sequence ID" value="CAD8179495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000072">
        <property type="protein sequence ID" value="CAD8179497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VRA9"/>
    <dbReference type="OrthoDB" id="590761at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF18">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E HOMOLOGOUS PROTEIN, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8179495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8179497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="208" mass="24699" checksum="AB9576B859688CE9" modified="2022-10-12" version="1">MAHKLSQNWVFWYAPRGRKAIAGSDHYDVNLKEIGEFNTVEEFFTYYCFLGRPSEIDIDNKIMLFRKGHRPMWEECLEGGTWIIHFKKRENELLNKKWEALVFACIGEEFDDDNVIGVVLSIRERRNLLEIWLKDRRESEKLRIGEKLRVALEMDPNNLTFFFKEHNKSLNDKSTMKGAESYTFVKTPLETPQTEPKVHHPDLDQFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VU36</accession>
    <name>A0A8S1VU36_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0710181</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000071">
        <property type="protein sequence ID" value="CAD8179299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VU36"/>
    <dbReference type="OrthoDB" id="10579761at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8179299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="275" mass="33115" checksum="BFFD083297812BC6" modified="2022-10-12" version="1">MTIVQSLIIDKIFINPSYATAGRGFHIATQRKAQYQLLTRIFPIQILYHTFQYFLLVDLPSGRSIESYQYFDECTYSLSITILLFQNHSFRQIQQSKQRIIRFKLHIKTYGIQFNFIIRSYTRYIQSIEISCFLNQNTRDYNLDDLVYWNPNYDQDQVLLISIECNEISEPFYRQDPPYFIQSSINNYKLLVDIRTFQCQLSEFLNQTPGWCRICDKFQNLLSITRKALNCSYKDNTKIRLIESSMIELRQYYWRAYNYSQNIEYCYQFPKNCQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VV98</accession>
    <name>A0A8S1VV98_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0730157</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000073">
        <property type="protein sequence ID" value="CAD8180105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VV98"/>
    <dbReference type="OrthoDB" id="295173at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8180105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="280" mass="33822" checksum="AB4424019137CAEC" modified="2022-10-12" version="1">MIKYNSENHFKFHNVKVFDKERMILYFKRMPIEQEENDNLYNGLNQLIMKERIEQTKQKIEIGKQVTSSVKMDQGELPKNRTLIIMNFDYPFDRKFIAKVFKLCGIIRRVYVGSMKVVDNNKKRLLHVGLVVFKNEYDLTKCFDIEYFQQKINNKFKSVPITTNYEKKLLQNYEFVDEKKNQIIEEAQKEGYQIAMNKYGDFETNIEKPKFEKRNWVGRNKNKTKKNINTEEIEQVIQGLDDNLPHQKKKDILKFQKQQLQQQFQQDIENLRQKQKKVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1VVY2</accession>
    <name>A0A8S1VVY2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0690216</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000069">
        <property type="protein sequence ID" value="CAD8178476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1VVY2"/>
    <dbReference type="OrthoDB" id="286559at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8178476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="276" mass="32478" checksum="B4C0BEBD885D6E07" modified="2022-10-12" version="1">MGTCQSQKHMGNPVKKKDQTSFTSQFTTIRELIHFFDQISLEKLNFPDLSAFLSKHIDKNRIVNEGKTWVAINEVKTILNNKTVILIRNFNPETDAAIQLIYLLKQLIEVSFGAQELQISFNENAVELFHYYQAQLVQISIEVLLAVNTLIRKQEQWWKDCYFNWRSSYIQSMFKIKQPIVEKIDLIQPFYEFIALLKQCAKQLCNQQKSPDLMEIDIKAQQEVLEQFYSQIRTGFLQNQSTQIDSINWERQNASKSLGQKLQIYNLYIKKKNYTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W0F8</accession>
    <name>A0A8S1W0F8_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0780121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000078">
        <property type="protein sequence ID" value="CAD8182293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W0F8"/>
    <dbReference type="OrthoDB" id="292613at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="296"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="320"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8182293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="432" mass="50061" checksum="094B8654E5F41D8D" modified="2022-10-12" version="1">MFRKVLGNSMRHNPTRLRYLREYVCLREATNPSAQQAQIAQFQQVANKYGLTTSQLTTLSGIYQLSQLLRPQNFPNPNDYKVHVEKLELLFRQKQLSNENLEQFAQSLQYRIPEISKLTNEARKQAAQDLFSIYLEGIHYDNLVSPGYVQQLESIFRQRTNAVINKIKAEVKDPQLAAQLAQSLESIFENYQFNQGRYVGLIKSFIESNQETEVQVSQKVDQQAQEKQLFIQTVDRATKQFSAEDQEYARNVDGHDFKLFLQELYEKEYQTGFAQNRFNEKGQKLVYESQAQEHHVLRYQILAGVFAGYFLYLFYRGDAYYSTILTVPTVAAAFFYLAKQGQAKRAIPFIRQVLKNTDNTYEVVFEQNRIISRIENVQAAQIKLAQDTAFSNTLVFGNPTDFGYHIKVADNSFIAPYLYSGNGLDFRTFINI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W3C5</accession>
    <name>A0A8S1W3C5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0810026</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000081">
        <property type="protein sequence ID" value="CAD8183493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W3C5"/>
    <dbReference type="OrthoDB" id="286901at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="315"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="346"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="435"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="465"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="588"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8183493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="613" mass="72038" checksum="612568837A6EB864" modified="2022-10-12" version="1">MSDDQVANELDDVEKNQVKVKFPQRSWISIIQNKIVSVNQISQSEFEDKHDTNIGITPGRSERESFCKSKHGSSLFFREDLGRSRISDFDPTSIKYRTQPHEVNFRIKVFIKYLALRILFSIIGPFLLIYGLFNKDWINFMKNFRIYGCTKGTAFNYFRWITNYIFFFSFVYYIGNYGYKRMHYEIGFFCILFVTENVTYAIKYAFFHPSKLNLIERFDMDVIGDLCEQSINPYHWAQQIPSMITKEFEQSMKRHQIEPTSFQLYFMVAPNPERKKRIQVGFTGGHQMYEDIAANCISMAREIVEEYNKKCQLRFINKFSIFISLIRVVAYIFIMNFCSESVKDYLIAIQMCIFQFMYFYMITVLMMITYRDLRRKVFAMTQLSHLISAEKVEVYQESKMFPTLNLLCCVSLYSWVNLRKMILDYGLQYTRRQSFMLSFIMILNAIMMVIMTLLFYVGYITINSILQQTVDFVVIASISIALVLQGARINAHWCIHRGLLRKNLFLIQQLSYQIFKSLDYTFKPEDPVFSAFQKVLDSATTSSVVQDDQFLNYSLFCIHSIQNVYNDLGQQEKCQPYTVMGIAMTYQLLLQVCISTMGLIGTSGLNFILQVTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W5R9</accession>
    <name>A0A8S1W5R9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0830169</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000083">
        <property type="protein sequence ID" value="CAD8184650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1W5R9"/>
    <dbReference type="OrthoDB" id="288275at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035847391" evidence="1">
        <location>
            <begin position="17"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8184650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="85" mass="10106" checksum="AF62EBCA95CF10C5" modified="2022-10-12" version="1">MRQVFIILILASICFAQSQLRGRQQFEKQDVNLDEYSFMQFHYNTPPMDVMMVNHELEIQRMMGVPGVVMMNYVLEESPYNPISW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1W716</accession>
    <name>A0A8S1W716_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0820238</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000082">
        <property type="protein sequence ID" value="CAD8184282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="326244at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767">
        <property type="entry name" value="CALCIUM/CALMODULIN-BINDING MEMBRANE PROTEIN PCM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39767:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8184282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="219" mass="25542" checksum="CEA24322D9B9B406" modified="2022-10-12" version="1" fragment="single">MIIQLVILQVVKTQIQEWDNYYKSFVNNNMSELESWFIAGARTQNYITVCSEQQILGGYYIFGMGTAVQQLFYLPPHYQIRIKLNFWRLLGKRNIKYYFGCSQGTIQYGYSYKNFQSPIEIIIPHKSETLVILLKTNLDNWTNEFWGFNNFYLDILKCAPDCLLCNENSSDICKPEMDIPINWISKVNVDGWLLRNTLPAQSTICLDKISLIGGYQILX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WF35</accession>
    <name>A0A8S1WF35_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0880228</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000088">
        <property type="protein sequence ID" value="CAD8187262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WF35"/>
    <dbReference type="OrthoDB" id="284103at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8187262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="127" mass="14813" checksum="983B6789A4B0560F" modified="2022-10-12" version="1">MDSYLTLELRRRLNSSMTYQEYLDAYDKPPPKPKLKPKESPVVAELLLLEPPKGPKPQSQKLEIYEPDEIIIEESETDERSRAQSPIQKPPCYKALKANNVRERSQSENLGFSVYRFLQNKQHVPKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WFF1</accession>
    <name>A0A8S1WFF1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0900172</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000090">
        <property type="protein sequence ID" value="CAD8188043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WFF1"/>
    <dbReference type="OrthoDB" id="296049at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46818:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_125, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46818">
        <property type="entry name" value="DOMAIN-CONTAINING PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="116"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8188043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="492" mass="58416" checksum="B3C145E84F20DC5B" modified="2022-10-12" version="1">MNKIYSQYNLERLRLQQSEKDSLKLERSDVKRGTGKNAIRKIFGIDEGSKHEYDIYEQQQLDAFKKEIEEERDLLTDEIILRFLYANHFDFPQTIKHMRNHQQWLQDPNNFKWSLNTEEMIKQGAIYVSGRGQGYKPIIVINADKFDITIYPIDDIMRAISIVLMVVKDYMLVPGKVESWYIIVEAKNTTAFSVPFTHLNQIFDMLKLNFPCYLERMFILQPQTSIQITWQIVEQFIPYNSRHKIEFVTNDFSLMFNYIKPKQLEQRHGGRAPNIYDYWPPHVEDFNEVEYLMKEQEETKKIQEQINQLQKVMPFTSSIDLQVSNLLRKHYKPKNKIYQEETHLETTNYYLNKSNSKTVFLNAPKVQQIISTQQRIQEQQEMQSQQKPQEVNASFEQQLNNNNYLNYPSQTQFNPNTQSNQLNFRQPQFQSRFIGSKTRLNETQNSQMLGSQNPNFQSRFVGPKTQHLFIKQDVSIIANKEELVSAQHSQIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WGY1</accession>
    <name>A0A8S1WGY1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0910045</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000091">
        <property type="protein sequence ID" value="CAD8188251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="76105at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052394">
        <property type="entry name" value="LRR-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1319"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1368"/>
            <end position="1392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1461"/>
            <end position="1483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1542"/>
            <end position="1559"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1579"/>
            <end position="1602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8188251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1729" mass="202144" checksum="24E0BEE37E993344" modified="2022-10-12" version="1">MQFQQISKSFTSKNTTQKKTIFHQEKQKQIEQEKEEQKDKAEADTYTIRFAKQMRLMIHQQQNVNLRENIANLKKRKTLEGFSGETPVSIQQCFSFLNSAIQNARSLRSLDLSGIEIDPLLAIELGQGLQKNSSLIEINLSGCKLNSFSLQYIFSSIANHQTLQSINLFNNQGFNIDLMNDINQFVFKGKNNLKKLKLTHCNISSTAMSYLLRNLKYSRSINAIDISMMQFTTDVLTSLGMALQHYKGKKVLEYLNIGDTNIRNQGVKVLAQRVSWNIRKINLKELNIRRNFIDQQGVPHLEAFLRKINNLQKLDLSQNQLEEFSCQNLLSRKMYEVNLSSNLISCFPQQFFFNTLIINLSNNNLKNQGAFQLSSILRQNPLWIELNLSNNQIKSEGFNSLIFGLRDNKMLKKFIVANNHLDGESIITYMINHEQVYFEHLDVSYNYIRYALIFFLLKFIKSGCLRCFRCSFQQKQSNDTWDININPNLFHEEDKFIKSTQNQFQLISFNLRELDFSKNENIFTPVISSLATYFNKIEILDLSSCKPVTEQDLDLLCTFLKKSTIVYDLNLRDLNIGSLNIESVKKLRNSILNSSLKKLNISKNHLFKLAKAFQIEEILTNYKLESLDMSYNGLESRHTNYIEKVISSYRNLQALNLSHNNIGFTGLIAISRVMQENKCLKSLNISHQKMSADDLLILSQIIQNENLQFLNISDNSEIKRLKTFFNLCQTEKMELLQLSQMHFDKSNFKNLLNIIKKQTRNILGITLNRCSFRLNDYEKFGLQLIKCKKLQMLCMTYNPLVYVDLNKTAAILNELSDLKILKLNQVSFTGESFFNVKEWILSPINCSLTTLDLSENLLRQEWLDELCIGISGNRTIQHLFLNKCNLGRLNLEPLGLAISQNLTIRILSIANNNIINNLCNSKFATVSKYKTVQFEELNLSENPLDENELIQFFTPQEQENKQKQATIIKQLNLSNCKLEISFFKTLVQEHQIHKEYFLFSHIISLSLSNNHLDDSIGELLKQTIIQSTQLQELYLQQNLFTSKGMIQIFEGIFATQTIKTINISSNKIGDLFALKLDQLKFHKFSVEFLLLRNNEFQKQGQKILAQILSFQKNLFIDNIWSNLDDEDADNILEQYTRICQKYRLDQTSLPSFLKEIQLSKSNLTDKFCESFGKYYPLLGFIEFIDVSDNPYITMYGKVYLFFHLFDYSYEKHQLRALHITDTQETRKLFDDGLLRYLTLRLRNYLLRQSKQNLKKQHPIQQQDNTQKETKKIFGGMLGSWLIHKINKLMTYLDLIEPQIFIVSTLFIGKFRRNNINIKIIVILFWIGFVVVNISQFFKLFIQVKNKNQSEIFNAEKNKEINNVIVTDLTYAFIIVGAILIIEILQLILTVTLRRKIQPALQIIHPKMVEHLHAKFPQKREIFLMIYSIFSKSSTLAERMFLASLMSLSDDIVDEDIIKFQILFSIVIIAKFADSFIITLINLIKFMFATPNDDQAKYLSLLQRNLYYQNYFVSSDVLVTLCPVQGYQLTKTIKVNYEIIIETYRMIFIELVLFIFIWTFSKNNHITGFQNFIDPKWPTLRLWMTFLFLKCITSIILSLFKILTVRPAVVKQNGFNQALAIKRFYQNKHQFVKPQNIQIEQNLQKIDFDLKIKQKQYTQNSRELEQRREIRIQKKIEEEIDQIDEEHEIIFSKQTSHQEYLNNSSLSDLPLQDLPQSPLSNYLPKFKFNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WKX4</accession>
    <name>A0A8S1WKX4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0970025</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000097">
        <property type="protein sequence ID" value="CAD8190608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WKX4"/>
    <dbReference type="OrthoDB" id="284125at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8190608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="253" mass="29281" checksum="3523ACDF8F9CA4AF" modified="2022-10-12" version="1">MLSETSKEVQKDQNVVPQLNQTPSSTYFVYNCYIPVVQDYMSQPPPLQVRNSSESFPQLIKNNDSIQLSHLAPGVSIPSFQFGASTPFVSPDDVMRNVQPTQNHFASFILNEQLKTQNQPTKNRIIEGTQTLCDIEIVLISRYFDMKAHVFCPPNNKYTLKEDTNISNAEWLKDRMLFRYKKPIKESFSDMIDQLEKEGEMNFKNMVHPVKFAQGGFDNIERQVPKSVKSEKPFSVFIFEIQQLISQFLFTKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WL51</accession>
    <name>A0A8S1WL51_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0970175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000097">
        <property type="protein sequence ID" value="CAD8190904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WL51"/>
    <dbReference type="OrthoDB" id="77931at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="505"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="547"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="596"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="629"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="684"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="748"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="794"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="824"/>
            <end position="842"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8190904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="950" mass="112547" checksum="EA4CAE42AA8C0A8B" modified="2022-10-12" version="1">MNLFSFDFKQHQNQIVVISIFNYQIKIKIFLQKQHGYLNKFCLYQVKRALQYIFKVQILTIDQNQFRYQYQIIIVQNVRMEQFNLIYNNIYQYRFKNYIVSRIIQEIMDVYTLDHKINKNHKQGAIGSAIMAENVRIAIVNLKLLNNIATYQGGALYFSINNNQFNISQTILYNNKAKQGGGIYLQGNSKLNQENFIKSWMKLNSASLLPNNLQEMPTHLSLSINNIEMKTIQKKINNKTSDILFLQPYLVMEQGKVQLTKLLMLPSNQVIINYKIYNPSQLKFENYLEEFSISYKNNLNEKLPNFSNSTCEIFMQTLINDTLIDSTNIANIEYHSESNNFDLRFLSITINPQKQNNQTNQIQIVCHLNDQSDTLLYYIEIKGFMCQLGEFYFQSGCQICQSSQGYYSVTYNTTKCSIFDQNKFESITSNQIQLKKGYWRPDYQSDLIEHLLIFVKEDGLCHMIYVQQDILEVCVKNVTCIILEDKEIIQKMKKIHLVKIVKMQLIVYFRLFLPQYALISTLLTLRSIDKSNKLFSSLKIRQGFVKIIFKLNQVIFTFNFQFQFSFTFINQTSNTSYFMANNMDCYLSQIYNISLVYNRIITMTVIMVCQLLIIYMGFKLYSIIKHQKFNSCIISTTLLYLYVSNYASLIKQLFSLLAKRIISDIEYIQGDVSLLYNSQNHQKWIIGFVLLGLGLIGFLIPSALFLLLYIQRDKLDKIKFRKHICYLFNEYNNENYFWEWIKLWKKTVIILIMTYFETNIFLKASLLGLCLLLYQLYAVRQKPYILNNLNELDVSTGQICSIAIFFATIKYINEQQGNKSTSVLIEMILVLLCLKLSYPFIIDLLRVYYKKYKILLLSNLYDALKIINCNLSLTIYCKQKIKLNEVKQFIIIYSYKENKNLKKIQENQEFIYQKYQNFNQNNKIIWIYQICNQHLDQHWLVLNTYRNQYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WL62</accession>
    <name>A0A8S1WL62_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0940013</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WL62"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8189415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="179" mass="20886" checksum="35E96244DA8AC0A4" modified="2022-10-12" version="1">MIVPHYHVVYGQRELINVKRTIAHYYNNLIVQKHLFISNGIRIQTLLYVQYLHHEQTQKLTAINNASNRILDALDLIKYQINAYIQGKQLLIVLICHQIYVHIILVNRDLVIGLMINVKYLINISMLSKKINVNSLIFSNKILLKEQCVNGMKLKINAQIQQVVVNLQVKILENTIFQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WN02</accession>
    <name>A0A8S1WN02_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0940107</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000094">
        <property type="protein sequence ID" value="CAD8189599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WN02"/>
    <dbReference type="OrthoDB" id="302851at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8189599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="327" mass="38594" checksum="0B5E40F44AA1D393" modified="2022-10-12" version="1">MLLCLNKSSIKSLFRSTNHSCESLTERERRRSTAENLDINTYLSSLQMMSDRKDIDKRIFKNLIQSKTKKTPLKQNLFASNSVKELNQSTQQSASRQKPFTRARFASQRSTILGKQAQAQDQYDEQNSEKLQLLNESLFKLVGQLLKLKDEMNNNRKFYLQIYKLIGLKKTLQKLLFNLETQTIIEPLTVYHLKQNICIKEHVMDGTTLLTLDTYNFNIIRDINFLIEKIDELQIQTEANGIRQLEYKITDEIYNLKMEQNKMKHKRVQSPKLLFQQRIMRNQLLPEGSMEQIEQKFGPFPLIQNKNKIIHNQIQTIINNLNIKFKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WP20</accession>
    <name>A0A8S1WP20_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type E3 ubiquitin transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0960084</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000096">
        <property type="protein sequence ID" value="CAD8190371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WP20"/>
    <dbReference type="OrthoDB" id="290022at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035821423" description="RING-type E3 ubiquitin transferase" evidence="2">
        <location>
            <begin position="19"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8190371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="251" mass="28430" checksum="83AE0BBA80E6B5FE" modified="2022-10-12" version="1">MLITNFCLVSASCAALRAVVLFRKEKILSKMFTDVDKMPLYNLDYINSKTDLPINQYLVLTGKITSNNLQPTFSDKSVKAILSTIVPTIKEKQKDIGEGLMINLGQVITFKRDHSIQFILGNDTHQLEVKYLPNRSLTLYNLPKLRENIMKSELSQIKIANQHFLQTTYTEFALIPNQQYGICGKLQQDGVLTPLIILGSSKESFIDSLEEELLKINKAAKIWLLCSGGITIAAAIYAKMYKRSRKHKENQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WTM3</accession>
    <name>A0A8S1WTM3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0930026</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000093">
        <property type="protein sequence ID" value="CAD8189086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WTM3"/>
    <dbReference type="OrthoDB" id="308168at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8189086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="247" mass="29502" checksum="A4D66D1FA16F2FC0" modified="2022-10-12" version="1">MNFSQCPDEFQIEEQQFIRVPQRSSLSSFNFEELDSKYKFTYPTNEKQNETKRNQLNQTPPPQTKQIQQYEIDKNYKLQIEQPNQLSKSPKQCHSFRDQGKIQLCQQQIEKPQKEIKSILNFQVDTKFINMKPSKKRNQKPILPQFQQQQYKRPLMIQSNPKYQRISPKNQNKNPIQNNIIQNYSEITFHQEPKVNKFQQLLNKAPKSIEIDHQRSIKWFGNKELCEGELNGKIVQFIKTQQSNVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WVK3</accession>
    <name>A0A8S1WVK3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1010097</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000101">
        <property type="protein sequence ID" value="CAD8192335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WVK3"/>
    <dbReference type="OrthoDB" id="289796at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8192335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="360" mass="42337" checksum="74EF9FFA53B77732" modified="2022-10-12" version="1">MGQEFYNMQNIKRQKSIFDQTFSQRATTTAESKRTCKIPIQIIDFKNKFETYLSGQTSCVDVIKYNNSLPTKKYRNSILTKRKTLHITNTITQQETMIDYNDIKEFIVNHDQNYTDIKKTLNTQPSLPERKSSKQGFRTYIKSDYQENKQLGHLNLIAEDYRQQIRNNNLVSSPDQLIFKNKIKARTKHMALIKPKLDSANMQSRIKQLKLENGTKQPIVDVYQKLFRATSDHFLDALKLNCNVRTTELNKMGELDFYELDNRQKAISERLPKKSIYGNQKSLKYNKNCPTNQFDDCILDESIISETEAKLNIFYGQSKDYQKKIMNRNENPNKVVNIISKIDQIRENIYKVNKNKLYCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WWI9</accession>
    <name>A0A8S1WWI9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1020117</name>
        <name evidence="2" type="ORF">PPENT_87.1.T1160103</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000102">
        <property type="protein sequence ID" value="CAD8192705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CAJJDO010000116">
        <property type="protein sequence ID" value="CAD8197768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WWI9"/>
    <dbReference type="OrthoDB" id="310988at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8192705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8197768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="103" mass="11802" checksum="FD8D9BF9649E8C4B" modified="2022-10-12" version="1">MASQAIAKDLYTYTNDESLSLMIYSIKGNQVCKDQRKSFNLCRSTPLGKHVEPEFCKDSALSFIDCFLGVQRNTKCHQQFQKVFDIAKTGQYAQESLEDYLKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1WY06</accession>
    <name>A0A8S1WY06_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1060126</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000106">
        <property type="protein sequence ID" value="CAD8194302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1WY06"/>
    <dbReference type="OrthoDB" id="297160at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052842">
        <property type="entry name" value="ER_Co-chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45184:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_102, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45184">
        <property type="entry name" value="DNAJ PROTEIN ERDJ3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="17"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8194302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="280" mass="33270" checksum="19CE2C6A6257A11C" modified="2022-10-12" version="1">MFLTRFRNLCKFSSVIDPYQVLGVDKFTPFPDIKKAYYKMAAQYHPDINKTPSAQKQFILIKESFEKIKVMKGEAVQMNSEGSVKKENEYQSVRKNYKDYDENFSESNEQKDYKEFYHQAEDLKGQEYDHLKKSFQTIHEVKDIKFKPIDMKMPDPSKRFHLGQVMHLKVFSDGSNLYIITICVGMFILFFVINSVFQMQTKRKTNSQLYNLLNQQIIKEDLEEEQLLQQVTTQIEKTQEFKQFKQEQLQKQEKSTQRRRQIVPEIQTFVSATDLKDSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X0A7</accession>
    <name>A0A8S1X0A7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1100108</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000110">
        <property type="protein sequence ID" value="CAD8195728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X0A7"/>
    <dbReference type="OrthoDB" id="290352at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="15"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8195728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="22524" checksum="145CE263E10988CD" modified="2022-10-12" version="1">MQNQIIINNFVQIKYLLRIIEFTQSLKYYILFIYMLTILKHNSMQNIKSLFQRNLKNKTLYFYTLNSDIHINITINIFIYINITQENLVQMNIQVYPMAITSEKVLKTQGDSSENVIPDSDQEDSLQSPVLKETPSSSSKPLKHIRFSGTNQLCYFPKSGKKDLSPSPTRKSQQSIQSILKNKEKVIFFTDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X1N1</accession>
    <name>A0A8S1X1N1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1070012</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000107">
        <property type="protein sequence ID" value="CAD8194495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8194495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="52" mass="5090" checksum="0839D58F06A37262" modified="2022-10-12" version="1" fragment="single">MLGNTPQEWTYTYLPQLLISGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGX</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X5J7</accession>
    <name>A0A8S1X5J7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1050007</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000105">
        <property type="protein sequence ID" value="CAD8193716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X5J7"/>
    <dbReference type="OrthoDB" id="71437at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8193716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="144" mass="17164" checksum="8C5ED833C606B42D" modified="2022-10-12" version="1">MELQSQDKKWIVMKKIWTSFQGNRLCFLNDKVFAFQPDNKKITQIYEINNTKKQYDKKNEIVLQGNDDGSMLFPQKFIKEQSLLVNKYYTFANLMRLDEDGKLLSKLTIEFNTYGIFGTISPDGEYLITWNYKTKEIEVRKYND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1X7I1</accession>
    <name>A0A8S1X7I1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1140085</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000114">
        <property type="protein sequence ID" value="CAD8197045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1X7I1"/>
    <dbReference type="OrthoDB" id="292483at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="214"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="314"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="388"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="3">
        <location>
            <begin position="491"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="703"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="731"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="794"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="819"/>
            <end position="830"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8197045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1018" mass="117982" checksum="F139F81C4A7DB0CD" modified="2022-10-12" version="1">MFDSSDSIIRNNLPDGEFKGPIHHLLSNRVVLQEGDIQGGSFNSFTVPENIILQNTPPRTPKNMERFKTMGGRKSEFQSAYEGEEVDSVKDSKPQFLRLIIAKSLQNNFINNLWNRSYLRKLHQLSAYQIEQLDDLQFESDAFSNITHLNQRSLIQSLAFWNLIDVFTPYSKFIVIWDVFQILTYIMIFFWLPYKISFEIYYISELFNGDNGSQIIEIVLLSILALDVVVGLNLAFIYKGQIIKDRKRVIINYFNQYAFVDLVNIKFYLWSPYPQFQLNSLFQTLVLKTATCQSFKQFFNLNGSLNDMVGLMKLLMIIIFIAHICGCTWHGIAHYTTAFSWLDAYNLRERTNGTRYNYSIYWATMTMTTVGYGDITAQNDLELLINNITMFVASIVFAYSVNSIGIFVSNMYKGTMEYSRSVTLINTFMSKNKIQFDLQTRIRSYLEYIWQEEQEMNDDEVGSIVKKLSRHLQDELQYQLRGNILKNCKIVMKLFSEQFLKNLLQSMEELSFSPEERIITCNQIDDCSLYIITKGEVELLFSGKNQLGDMIKRNSIKLLKQYECFGEVAFFTGNPRTATAISKGFTRAFKIKRENFLAILQSFPNDYEKFCDARDRLINNEFSSLQLNCYSCNSNRHLINNCHILHFCADQEKILKKELFPVEQRRSMAFNRIKKTKAVCAFTMQKYYSGRAHDLIQDLYQQDRGGTDIDDGDSHTMPMNDAYEDDDDLPSSMNQQRSQSRTMSKLSSKQPQQQEGEDEEQQNKGYLRKSYPPRQTVQTAGFGGNTKNKDITWNVEQLSDSLNSSEEQDQVIPLQSHPQQKQNEQNNKQQPIKRQLSRSGSADDPSKVPTEIRLAKQQISQKNQSDLEIRHALSREQARKITGRTRTHNDDPTLITDNLTQNNAGQLLQAPTITTIVLAFDKMCIFQFYQPLNNYDAVIKRYARAQKFFGKKRLYPETSLYSFYFMAIKKGYKLKKLGQSIPNGLQRFSAGAKLLKKAVKRIKQPTLAEKSDIFVQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XAZ2</accession>
    <name>A0A8S1XAZ2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GB1/RHD3-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1180015</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000118">
        <property type="protein sequence ID" value="CAD8198214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XAZ2"/>
    <dbReference type="OrthoDB" id="2135133at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01851">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030386">
        <property type="entry name" value="G_GB1_RHD3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003191">
        <property type="entry name" value="Guanylate-bd/ATL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015894">
        <property type="entry name" value="Guanylate-bd_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10751">
        <property type="entry name" value="GUANYLATE BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02263">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02841">
        <property type="entry name" value="GBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51715">
        <property type="entry name" value="G_GB1_RHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GB1/RHD3-type G" evidence="5">
        <location>
            <begin position="34"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="453"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="602"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8198214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="680" mass="80090" checksum="4FFF39F989358119" modified="2022-10-12" version="1">MQQELDQPILFIDIDEDGKFLMSKKAVQFIQAIETNIAIISIVGQYRTGKSYLLNRFAGQQTGFTLGSSTNPCTKGIWIWGKRVESDNLTILLLDTEGLNSYERDQNNDARLLVLACLISSTLLYNIMQVIDEKSLETLSFTTNISKHLLGDDEFITQYLPSITWVIRDFGLDLQGSTANTYLEECLDEQQGFADEFKQRNLIRQAITKYFKRRNCYTLVRPVADERQLRELDKVPYSELRYEFKIQLNQLIQNTLLDIQPKMYKNTPLNGKRLIEIVTQYVTTINNGSVPNIENVWDRILSKEFNKMMEKAKEIISSDISLPTTYELLFSNLHQISTKAQESILHYHIAPDRQIEGIVQLSKQITEKSQVLFQENYIKSKRTNQVISNKMYDILNQLSEQGKLVNVQHIIAQVNNIKKIYFQEAKPPSNYECYVQFLELVMQIIEKVSNTMQMNYQRKEEDYKQEINVLKSQINTLQEILKSEKENLHKQLDQVKLTLQEERVMYEKKLLELDSSSQIEFRKGELFENPDLFMKKELKNQQLQSQQTFNLTLKDINQKFMDLKEKLDDIYESRVEIEKEKIYNKAIYECKEQNQAQIDRIKLTYESEVKILKEDRLRLQEQRELLQIQLTQKDCEYQILKERLKQIEKQKTKDIEHADMLCKISDQLVKALKKLEKKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XDK1</accession>
    <name>A0A8S1XDK1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1200082</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000120">
        <property type="protein sequence ID" value="CAD8198943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XDK1"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8198943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="96" mass="11424" checksum="933BE6821810E915" modified="2022-10-12" version="1">MLSSYILLQDIYTTKEYENLDLEVIESISFIKIPYYNKDDKTVLYEALSITFNYYDSYFTRTNTKLYKYISNLSSCNVLFQKAQAYEISIVISFQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XDZ6</accession>
    <name>A0A8S1XDZ6_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1210003</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000121">
        <property type="protein sequence ID" value="CAD8199103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XDZ6"/>
    <dbReference type="OrthoDB" id="317064at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="58"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="170"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8199103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="409" mass="48065" checksum="085C94105B835945" modified="2022-10-12" version="1">MYQPRRLSPASSSPLYRNVNVNVQQQNQIKLQLKQITPTNNIPPYVQIISQNHHKQNTYNSYENNSRQVSKERESTSSNQSKDQIFNQYQYESVYQNQTQSLISKNDINSIYSYIDNIIKTQKSEIFKVLDHQNKQIEFLQQIILNQTQYQQNASSQFLQLQTQLEEKLSNFINKQIKELSNRLQHLNKNEIETMNSQLEKLRAQIFQINITNSKLITNKLQSSYANDNENQVINHSQNHKPFLTDNQSHNIDSNIKIMKNNYLIDRDFSTQSNNPLNDITNIQIKYIKSLQQSLQNSKLSKCSSNNNYKQQEGSPIELFNEQNILDSLDNIKVFDPQHSDHPSDSTNNYNSMDLSQARMSTIQENENEEDECVYQVVENRQQQTSIENKKKQKDQTKIKQCKSKTLYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XMS9</accession>
    <name>A0A8S1XMS9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1300181</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000130">
        <property type="protein sequence ID" value="CAD8202331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XMS9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8202331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="84" mass="9388" checksum="57EABB62DE71AC51" modified="2022-10-12" version="1">MEKTHPECQVFSPQCTTNGAIYIPITSYTSIQLKTSCAVGTDGTCGCFTCWKMLDFCIIHRCYCQLAKVILQMDVVAKKTRTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XN57</accession>
    <name>A0A8S1XN57_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T1310054</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000131">
        <property type="protein sequence ID" value="CAD8202471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XN57"/>
    <dbReference type="OrthoDB" id="449424at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14003">
        <property type="entry name" value="STKc_AMPK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000956">
        <property type="entry name" value="CAMK family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF30">
        <property type="entry name" value="MATERNAL EMBRYONIC LEUCINE ZIPPER KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="104"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="451"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="451"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="474"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8202471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="527" mass="61443" checksum="657DBA676D0312F2" modified="2022-10-12" version="1">MKHRAATNSLNQKENEKCIGNQYALKYRPPLQQDLLMNLKNQQLKNLDAISQILQSKFCKVEKRDNVEYFKFDFKKQNICDRVKFLINQPDDDTNKTEQFKAQYKVGSRIGQGAYATVRIAIQIESDTKVAIKIYEKTKIKDLQRRKGVRREIEILEKLDHPNIVKILDTVESNNHVNIILEYVSGHSLHHLVRKQLEKRLEEEIAKGIFKQILDAIQYCHSKNIAHRDIKLENILLEGLNPKLIDFGFSTSFPIEKKVKMFCGTPSYMAPEIVTRQEYRGDKSDVWALGVVLYTMLQGVFPFKGDTDSELYNKIQSGEFTILHDISKEAIALLYGMLTIDPDERPTVVELLNYPWFKKQQQNQENEDIKKKHKLPDDLQEDLNTITKNMSYITPTSQIKQQLYFDFSHFKNNSKQNNTKAIIPKPTYFTTTNAQTKQREPQFFKVDKILTKNERHTTTHSKERQPTLSKERQSSYSFESSRGLSQKKNPHQLTVHPSFIPSEKSFNSVKSPSVGMTEKRSFNFFYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XPM5</accession>
    <name>A0A8S1XPM5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1330011</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000133">
        <property type="protein sequence ID" value="CAD8203065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XPM5"/>
    <dbReference type="OrthoDB" id="10616923at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8203065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="356" mass="41961" checksum="160FFD15B1CF2CAA" modified="2022-10-12" version="1">MNHKLNSFEDQKQNPERSQALYNLIQNCLWNSYAFVSIQNEQSQQILSKQTNGSRHLSIVTQYCQNCYMREGDQLYYAGQMEGDKPIGIWRINIDSSLINKFEAKTIFLELQQNGIALLIEQGQIKQFSIKLINQLKKKLFSSKFKQEAQIDQIEFEHSIFTKFKDRVLGCQIDNSEEYYTHQLLCQNLILNDFYFPKQQKISIFRMHPPSIPYFKNLTINLDGSEVVCFGQQYEGKKTGEFFIKFRNDSDSEFQTMYSSAQIKLVEVEVIKQMEKNMEDGQRFQNFFPNQTKSLLKDNLIMELNVTIFLLRQEKILMKCGKKLEGDYMINKEDKQVLGLRNMINQSNNLCLQKFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XQN3</accession>
    <name>A0A8S1XQN3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T1330156</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000133">
        <property type="protein sequence ID" value="CAD8203353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XQN3"/>
    <dbReference type="OrthoDB" id="424834at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052187">
        <property type="entry name" value="MFSD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23512">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23512:SF3">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="129"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="195"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="259"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="297"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="357"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="388"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="429"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="39"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8203353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="546" mass="61825" checksum="89963B1482AC74BD" modified="2022-10-12" version="1">MKVNDSVENNVNKNTVYSEQVDVEQEQKQEKKSSLKYIMLILACLLMFGNNYSFDNPQALQRQLTQDLNISISKYNLMYTAFSFPNIFLTLIGGVIIDVLGIRVAILGFSAIVVIAQTIIAFGGFFKSFWIMLAGRVLFGTASESLLIAQTAMISKWFRGKELSTAIGYIMTMPEIASAANSFVTPTIYDHFNGLTYPLFFSVFLCFLSFICGVVLCILDKNNDKKQKELQFVYKDNTSEKSEQVEKVSFKDIKSLNGTFWILILICTLSMGSYVPFLDDANDFLQEKFEFSYVQSGRILTLTYLAAAITSPFLGPYVDKVGKRRFFILITCLFFSVTHFLFGFMKQGYHDQPNWFSIIPLIMLGMSYSLYCCVLIPSVQYVVQQRVIGTAFGILGMFTSTSMALFPILAGYIVEGATTKEEGYSTVGFFYSGVSVFGIIFTISLYFFDKQSSIILDFVNPENPTKKEQEILKNIKQENNEQLGVFEDIDDDLLKIEGEENDDDENIKNDGFWEKNQRYLVSSKGFSTPKLAANRAVHERSGSLYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XSC6</accession>
    <name>A0A8S1XSC6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1340011</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000134">
        <property type="protein sequence ID" value="CAD8203462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XSC6"/>
    <dbReference type="OrthoDB" id="4726at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039539">
        <property type="entry name" value="Ras_GTPase_bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10693:SF20">
        <property type="entry name" value="AT27578P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10693">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="40"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8203462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="156" mass="17875" checksum="C6C116A566A4A50F" modified="2022-10-12" version="1">MSNHEDDLEVQEPIDQLKEDESQKKGGTDNKHQLKDIDLSSIFVRNLDENTTEDDIKEYFKDCGTIVKVTLRSDKNTGTLYSYVQFQEQASVDDALVLSEGIIRGKKILVFQKRTNLRNRGRGNRGARGQPFQYITRGRYLYAEPIRGRGAYRGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1XU48</accession>
    <name>A0A8S1XU48_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1390015</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000139">
        <property type="protein sequence ID" value="CAD8204859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1XU48"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8204859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="173" mass="20600" checksum="65207F36938FDF07" modified="2022-10-12" version="1">MSSSQEAGKSNLVNQITKGKLLDKYEYTKGIDGHVKLIILDNLDKKLIKNLFTNIIRKQIVVFQFMISLTMKEQNLIEIDEYFFFNFLPEAQSNFPVILVGTILDLIQKKMMKNRQIYKEMYSLFKCHQKTQSLFKRFYKLLLSYCEIGASKIKIIRKRYFKSYQQRFYICIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y222</accession>
    <name>A0A8S1Y222_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T1480085</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM161 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000148">
        <property type="protein sequence ID" value="CAD8207720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y222"/>
    <dbReference type="OrthoDB" id="304867at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044782">
        <property type="term" value="P:cilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051655">
        <property type="entry name" value="FAM161"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019579">
        <property type="entry name" value="FAM161A/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21501">
        <property type="entry name" value="PROTEIN FAM-161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21501:SF1">
        <property type="entry name" value="PROTEIN FAM-161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10595">
        <property type="entry name" value="FAM161A_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="617"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="673"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="779"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="853"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="445"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="620"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="810"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="867"/>
            <end position="883"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8207720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="894" mass="108263" checksum="22CC92F0586EF27D" modified="2022-10-12" version="1">MTDQLFVGQLQQKVGELKTEYDLQRSIKDNTINAKIQRSRDKICEILRRVQSKRPKLQTEESEESLDGNIPELKKPYESKYNEEVLQRRHYIFQFKMYLRRKRERLQREQEFKLLKLLDQQTEALQKWVTKDPNDLDMFDFFYTQIDPHRKGQVHHLQLKNFMYENPLIMTLFQFDRQSLQLALDSFPVKERFHLKQDEFIQFLQKYQKIKPEKGFREYVHLFDPIHKQVNLFENNPRILLLEDISKIQEAFDTVSENGLATVDEAIKSIRQNCELFVYAVHVVVFGLNLLLESVLQQIEIGYLNNPDEQISWNQFMYFFDNLPNDQDEEPLPQQQEEQISSESSDDYFYKMTAKYEIPPPPETEKKKVKKQSIPRKPFQEINVDRMQFTIPKKTGQEKRDVNKQPSIREKWVMEQVRQKQEEIEEAYKFRPFKAKAIPYKVKDKNYYNELLEREEIRRRELKERCKEKTQSMQKPFNFEDRKREQPIKLPEDQEEPYKFRANPIPWYCSLKLYERNIQEQKIKSENRKKIRKFWLQENSKLPPRMQEWIEKMKLKEQEKQQELLIKSYQESSRKVRAKSIPNFNKLHDQFQKQLDKKKKKIKTTIPIGPIFHESKKKAQRDYLDEKSIQKEDPLKKAKKAISTKPKIQPRSTDKFNAYVDYIFTMKQNQLALDQQNQEEQNQREDKKQLFRPRVHQSWAIQDHNQVQYERQQQKFIEQYQKRRQEEEDFNNMMKEIFVRVYQRPLLMEVEAAKSQQQPQEEQEQQEQFDYMNPIGEQDVEESERISQQDPEGVDQVGEQAEQSLREYDGQEIDGEYDDQIDIDNLDPKLLEKLQKDAIAKQYGLTEEQMEQIEQMGGFEAVQNMGYEEDDDDDDQEQDDQEELQNPHYKRHSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y2I5</accession>
    <name>A0A8S1Y2I5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1480089</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000148">
        <property type="protein sequence ID" value="CAD8207725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y2I5"/>
    <dbReference type="OrthoDB" id="290660at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15571">
        <property type="entry name" value="ePHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050701">
        <property type="entry name" value="Histone_Mod_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793:SF107">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793">
        <property type="entry name" value="PHD FINGER PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13831">
        <property type="entry name" value="PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13771">
        <property type="entry name" value="zf-HC5HC2H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="265"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="5">
        <location>
            <begin position="615"/>
            <end position="665"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8207725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="713" mass="85045" checksum="172B275F300EF051" modified="2022-10-12" version="1">MFPSYDDMQNREKIEEQYVLDPIPQEQEDKPIHQNPANNGKTYQQLFEIVQRLNDINWVEPSSKITPIKYLTPITYWFIICSQYFQNNVDPKIIKINQECHQIQEETARPYTYFDDWFIMEMRNITELEQQLLDTQLQVNTQIDAIQIEKSTLNIQNPPDYILKDLVGLTDIENQYLTYLESQQKYYDEQIINQTKSSNLAGTARKLQNVYNNCQICNQGVRSEDPLISCQKCQIVVHQKCYGLENAVNNWICDVCLNFGNKGRYLKCPLCPKLGGAMRPTSMSMKDSIFELINPTFHTYAINYKVDRQKPPPDGEENYNFMILQYQLENMSDEPPKAEKIWTHVSCSLWLDFDLVKVDKRKFNSLCSICKQKKNGACVSCSKSKCTISFHPECARRSQIYMESDNIYCFKHQPLKIKRIFEDQHNQWKEEIYSFFKQYEKLEQLLAHRPKNNDIEFQKFELKAQQEEIEADIKQENELLFQRIAEILEKDEKFIITFQQNQVVDIQLPLKRQSIYDIEENDRIWQQLANEKCSSEQVYILYQRAIRMRKKKKQGNAIIQLQMPSIQELPNRNRHSIYSRPKFFKYHKKQKQNGVSNNNISLKIKIPKEAINIQQQYCICKQQNDEEMMQCEICSEWYHLKCLGFLSSLEDAQNLYFYCFRCEQKLNREQTKYIKRYKQYFIDATFRDLKLKIGMSPHELRAHEKKNYKQLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y362</accession>
    <name>A0A8S1Y362_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T1510018</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000151">
        <property type="protein sequence ID" value="CAD8208476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y362"/>
    <dbReference type="OrthoDB" id="258495at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12885">
        <property type="entry name" value="SPRY_RanBP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044736">
        <property type="entry name" value="Gid1/RanBPM/SPLA_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="SPRY" evidence="3">
        <location>
            <begin position="135"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8208476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="310" mass="35921" checksum="626FBF442300633A" modified="2022-10-12" version="1">MQNKFLNHITQLEAENSTSSESHWLLITGIILGSLIGIAGLVFGYFKYRQYRVYQEYSQRPENGIPKEKIGFAQRNKFRFWKFYENKDEPNDDLVKQQQKENYYGQYIELTFLCKPGDTLTIRGDKPVARDAISYFEIEIEDNPRNCDIIIGFCSSKEFQKQFSLGRSQSSVGFHSQTGQVYWRSNLHQDHKFQAVYGETIGIGIRQKDGRVWLSYNGKFLNPPPASEGKSKEDMEKEKKKEEADSEIDIDLNNKNKEIEETRKALILSMGQNLELYPAISVNGRCKINLNVGGMQFRMNQKELKQGLLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8G2</accession>
    <name>A0A8S1Y8G2_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein translocase subunit SecA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T1600120</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000160">
        <property type="protein sequence ID" value="CAD8210776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10067052at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017038">
        <property type="term" value="P:protein import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000185">
        <property type="entry name" value="SecA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011115">
        <property type="entry name" value="SecA_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014018">
        <property type="entry name" value="SecA_motor_DEAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30612:SF0">
        <property type="entry name" value="CHLOROPLAST PROTEIN-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30612">
        <property type="entry name" value="SECA INNER MEMBRANE COMPONENT OF SEC PROTEIN SECRETION SYSTEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07517">
        <property type="entry name" value="SecA_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00957">
        <property type="entry name" value="SecA_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51196">
        <property type="entry name" value="SECA_MOTOR_DEAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SecA family profile" evidence="6">
        <location>
            <begin position="2"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="4">
        <location>
            <begin position="156"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="5">
        <location>
            <begin position="502"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="domain" description="SecA family profile" evidence="6">
        <location>
            <begin position="857"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="5">
        <location>
            <begin position="930"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="823"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1251"/>
            <end position="1402"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="73"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="2491"/>
            <end position="2528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="823"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="853"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1251"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1281"/>
            <end position="1402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8210776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2620" mass="304758" checksum="AA1710DE08F52716" modified="2022-10-12" version="1">MKNFFEESFLSELKKKIQQKIKDEDDQQCIFQQLKKINDKKIIESIINKEFNTIEEFFDIKKEYQMKSQTNEGDQHQMKIKNLFEQYQSLNKEIDKEIYNQLCQVYNSLLNPHFLQADFEKSPILATKDLVVLCNKVMQHFSYYPKPVQLLSVIELYNHKQNQGRLAEIYTGEGKTLIVAMLAILLCSKKQINVDIVTSSPVLAIRDAKQSEQFYKLCKITVAHNIRESQNEEDMTERYKSQIIYGDSHSFQADILRQEFQKVSTMGDRKTQYVIVDEVDSMLIDGNGSRTQISSSTPGMQSPTNVLYFIWHQLCQVKQNSSNKKFLCYQDGNFYEANLEEYLYQKVNKLIQEILKNTLSDHQLDYVNFSLKVWIKNAIRANFELQENVHYVLDPDVRITDYMNTGVIHEKNTQWQNGLHQFLQFKHKIPVSPLRISTNFLSNVGFFRKYKIILGLTGTLGSQPTQNLLANQYNIDCVFLPPDKKRLFKEYPGITTLTKEDWLAEILKAVQQEKDKKRSVLIINQTIQDVQEVKEYLAKNNLTSITYIDNKKNLKKNILPLEIIIATNLAGRGTDLITNEELDRNGGLHVIMTFLPRNIRIQHQGFGRTSRQGKPGTAQLILQVAQDFYIGNIDGIKNFNDAIVYYLKQKQQRDHSYFNILIYFRDQYERYHVDNMEKEMKKLYNEDKCFQVFQESVNNIDRENNYEDFKIKEEKFGRYFERNQENGLKEEEVKELINEKNIQNARFHVLEGIKQNDGNKFAQAHDIQNNISSTNYYKAVFGLQSHQLKKEEAQQYLNQAREQLQMEIIIKDLQIQFVKEEFDEETQSNTEETDLTIPKVSKDQKIPNLSEKNNNQQQSNQTNSNNQQQSNQTNSNNQQQXKCLTGTLGSQPTQNLLANQYNIDCVFLPPDKKRLFKEYPGITTLTKEDWLAEILKAVQQEKDKKRSVLIINQTIQDVQEVKEYLAKNNLTSITYIDNKKNLKKNILPLEIIIATNLAGRGTDLITNEELDRNGGLHVIMTFLPRNIRIQHQGFGRTSRQGKPGTAQLILQVAQDFYIGNIDGIKNFNDAIVYYLKQKQQRDHSYFNILIYFRDQYERYHVDNMEKEMKKLYNEDKCFQVFQESVNNIDRENNYEDFKIKEEKFGRYFERNQENGLKEEEVKELINEKNIQNARFHVLEGIKQNDGNKFAQAHDIQNNISSTNYYKAVFGLQSHQLKKEEAQQYLNQAREQLQMEIIIKDLQIQFVKEEFDEETQSNTEETDLTIPKVSKDQKIPNLSEKNNNQQQSNQTNSNNQQQSNQTNSNNQQQSNQTNSNHQQQSNQTNSNHQQQSNQTNSKIPNSSQNNNNQQQSNQTNSNNQQQSNQTNSKIPNSSQNNNNQQQSNQTNSNNQQQSNQNNHNQQRRNQNNYAQKEKLNNQKKIYQKIIDNIDDILNYLEFADISKFQFSWVPTIDINNSADQCMKDQLEIVEDGPFPQIVKIHSEENSKWWEYLGLFSLGTLEIILGTVATVVGAFTLGYTFPLAAGLITSGIQDAVLAIVGYFKNININWNAWCQKTLQMLTYCIMPSYSMIEKVNDICLSFAKISLAEYLIQIPPISRYQLANAFSILCDEDKIIIQNLRQTLILILKGIINPENAKSKILQLAKNIIDDQQGKNAITKIQASDIYQLVEISLSSSGAEFENNFCEFAQKYIQLQINICVEKKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTNNKAIKPIQITNNKAIKPIQITNNKAIKPIQITNNKAIKPIQITNNKAIKPIQKFQTHPKTIITNNKAIKRIQITNNKVIKPIQKFQTHLKTIITNNKAIKPIQITNNKAIKTIITNNEEIKIIMPKRKNQTIRKKFIKKLLTTQMISQIIQNLQTFQSFNSHGFQLLIQITQLTIEDGPFPQIVKIHSEENSKWWEYLGLFSLGTLEIILGTVATVVGAFTLGYTFPLAAGLITSGIQDAVLAIVGYFKNININWNAWCQKTLQMLTYCIMPSYSMIEKVNDICLSFAKISLAEYLIQIPPISRYQLANAFSILCDEDKIIIQNLRQTLILILKGIINPENAKSKILQLAKNIIDDQQGKNAITKIQASDIYQLVEISLSSSGAEFENNFCEFAQKYIQLQINICVEKKNDMQIKQDFVDLCKKQGKRKIESVEDAQKISEQKKKELFIFFEHLEKNLWECFGKDETIQKSLLTQQIFVQLCYDINNFQEKITYSQIWALFTQQYYEQYQQHKIKLKQKILEEPKNINYFYNNIMQPSIQNALKSLFINLKDQLEREPFEIQFEFNLKQVIDEKQQKLQVEKKILIEQQKSQDTQQKRLQFKQKVEKFNQQSEELREIIKNISNLEKIIKYLKNNKMINNDGITFKSCFQEELNKYMIKEQFKPPMKELLLQTLMNIPSQIQEFQKTEKQFEKNVRKAIQEIIICEYKNQILQIYQDEEEMFRKLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1Y8S8</accession>
    <name>A0A8S1Y8S8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1580030</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000158">
        <property type="protein sequence ID" value="CAD8210149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1Y8S8"/>
    <dbReference type="OrthoDB" id="421226at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="203"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="274"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="546"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8210149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="926" mass="108910" checksum="B0B826A2E80CDE13" modified="2022-10-12" version="1">MQYSATSKCKLMSPQIEQEMNSKGLDNSILEMSPMKIETSNPGISTTQQLVSAQKFGGTTMRINSIPLDTLQPPDLPESEGVLSIDKQRETQQMLIKQKSPFAKLIINCHSLKFANKMISFIRPDCKFSQKQHEILNDQASSYMKQKGKISGNFVQQNLNTIDLQIRLKIRTLKNKIVNQLSGCFSQLYNYIPLIEPNNINKLIWDFLLCLIRIYLIIWSPIIISFPFLQNDHANYFLISCVFLVFDIMIRNFTIYFHKGLPVKDRLQLFKKQINFGLVIELGSILFGILVSLDYQLSTWFFILFYYQLRNIMKLVDIIQYNLKPTKMVSSIIDLFKLIATVLLIQHFCGCLWLKLGQYYDSQGKMNWMIDVKDESWQVQFLESFYFMSVTMFTVGYGDITPTNSLEKMFCICYMFLSTLQYSYSVNTIGLILTQMKENNEMIRQKMTCINEYLHCKKLSTELSMKVREYFNFYWNQEIIQKKNEQIRLITLLPEDLQKKLKLESASSLIIKCPYFSQFPKSALECLLQSTEFNVFQPGVYINPQEYIYIIENGKVEVMEDKIVIDTLKENQYFGLQELFSQTDKINYKSADFSSLLMIPRSEVLKVISKFQLQIIPDKYCYICHDKRHYTHQCNVVHYIPDIEKVIMRYHFLNIQERTTLKQTKKIRSKFSALNEQILIQDSAKIYQLENDIIPENDIQEQQSQGFSIHQNGPPKINVVTFEDQQGQLNPPEWLVETSFNPAKFMQRTSLLQQTQILDSFKNKREKKLLLSGTTNKQSDQTILSEKPGDCQETLNKLQTILPKLSNNEYEKCFLLIKKLEGQLGLTEIPDFEQLKEFDKFDREWNIDRIITKLMRPIRKNNTELLNQLLNKYSKYLLFPYEFIRLFRNSLIEEEDIKQNKSYLEQVRHSVRHFLKRKATAVFPIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YB40</accession>
    <name>A0A8S1YB40_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T1590051</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CAJJDO010000159">
        <property type="protein sequence ID" value="CAD8210408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YB40"/>
    <dbReference type="OrthoDB" id="354826at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05578">
        <property type="entry name" value="STKc_Yank1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000454">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000854">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF374">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="24"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="11">
        <location>
            <begin position="283"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="357"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="357"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8210408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="385" mass="44337" checksum="450D3AC4F45AFCD1" modified="2022-10-12" version="1">MGNCNINEKKEDLIENNVMSVQNFQFIDAIGRGGFGKVWKVRQKKNKQFYALKVMSKPKIISKKSVQSVMNEKALLCGLKHNFLINMQNSFQDREYLYLIMDLLSGGDLRYHIGRHRRFNEEQTKFFSACIIVALEYLHQQGILHRDLKPENLVFDSNGYLRLTDLGIARIWKPENSQDTSGTPGYMAPEVMCRHNHGIAVDYFALGVIVYECMLGRRPYLGRSRQEIREQMLAKQAAIKRQEIPPGWSLEAADFTNRLLQRKPQNRLGNNGPDEVKEHPWFKDFSWEKLINKEMIAPFIPNGNEDNYLPSDSRRDSDDSINEDQQMMLRRNSIQNLFNGYDFDNNVSVSSSQIAISSTSSSRVTKQPTTTTTPKSAKLAQKLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YBE5</accession>
    <name>A0A8S1YBE5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1630061</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000163">
        <property type="protein sequence ID" value="CAD8211395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YBE5"/>
    <dbReference type="OrthoDB" id="286632at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8211395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="382" mass="46390" checksum="DDF404BDA027A2DD" modified="2022-10-12" version="1">MNQPYYDILSYLKQKDPTIRNQLIPIRVDYDEYQEQIIRYFKSIFEQTSNLTSDQQLEIEIKLGTFLSKEEKYKEMIDNFRFASKYHHLLITQQSQNYTFQPNFVLYHQDKFDTNSLSFSFINLTQYFRNLEKNDEISSKIYNMYIDPEKNAPSLKQQYGLRLDLLLQNSQRVSIYLNGKKAIIQKKRLENLDIMKDYIQYRLSSNIENNFNIPIDFDNFIDRNKFRSIRFKQYQIIQYQFLEISLSRVLTVDHQNFSQLKDQFKADGQIQWNKLLYHLLFKLYDVNPEFKYPTMRDNIINNVRRCEIEFEIHDINFFRQNLTAFARMQRNVDSFFYCDTISADILYNQIQKIHPKADRRKHCTPLIGNYLQTVYRVANKYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YCD5</accession>
    <name>A0A8S1YCD5_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase C1A papain C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T1550006</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000155">
        <property type="protein sequence ID" value="CAD8209492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YCD5"/>
    <dbReference type="OrthoDB" id="190265at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="2">
        <location>
            <begin position="26"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8209492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="256" mass="29317" checksum="99AA805F973B39B7" modified="2022-10-12" version="1">MSQEEFAQTYLSLRVSKTANDNFQYKGAEVDWTNDKKIKYPAVKNQGSCVGTLEINADIELNKRYVLSEQDLVDCTRPYGNQGCKGGWVYQAYHYILDFGLSEAKNYPYTGKDELCQFTVDRPFKHLTGYYIYETCSQLVDAIQIRTVAVAVDAKPWQFYASVVLSECTKDLNHAAVLVGISADGVWKIRNSWGATWGEAELLKVILVVYVLNHVPLYYNEDYSYINLSTISVILQTIYLIEQLHKKLISFFNIYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YCV4</accession>
    <name>A0A8S1YCV4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T1630090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000163">
        <property type="protein sequence ID" value="CAD8211451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="77931at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2355"/>
            <end position="2373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2414"/>
            <end position="2435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2466"/>
            <end position="2491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2503"/>
            <end position="2523"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2549"/>
            <end position="2576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2626"/>
            <end position="2646"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2690"/>
            <end position="2711"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8211451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2821" mass="330499" checksum="DAED40E32C73C8F4" modified="2022-10-12" version="1">MKMTSVFGTIKMNPTNQYLRKIFEPKENEYSSNSCITYGIWSKYNPLSTITQIGKFGLFDNYCFHLHNAIDQESKSLNLIYYDCLDSVSKKITKTLLFINNSDEQNRFEIIINPFDYENTWFYLQIVVWPLQDIFKLIIMKRIEILLETTKQMKYPFKDKNLILSFGGNFIVRNSKIHDLQIDQIFTYFPGPIILQDLQISSINLNFSFIDSANKVYNSIKTCVCSQNAKFLIGDMDVKNQDMNTFISEKINCDSFILVGWIKVKEIVNPSQQLTYQLIKISGNLLDPIFQNQNLSPFQLFYHFSADKNEIEITTYSYTFPDVSIDFSNNSFLLQKRFLIQNKISLWHYLKVELQKNKFSVKILFYEGKTTFNYDANFDVRQFQNCQFKVQYGNILQTKTNYLNIQMRNLEFYNCYKKLTQLSCHYSCLECDGPTNQDCLSCSIESQRTYLPEYKVCICPFNTIDNQNQCQSYQQSNLRLIKDEDSIISQNCKYGYFEIDGECVKCPSMIKEQFISCFECVNNPKSWFEYPTCQRVYVVKPNITFEEAYIPFGQIQYYYDGIQINPIHYTHSLAIQVNFPNINGIFKEFQLASIYFRQFCQQMDFQESDQFICYECFIQNCQVCQLTLTTLECIKCYSNYKLIDGYCKQQSEINQKSEFICLPPFYYSFDKQCKICEIKNCIYCFEYQLNDLNVCSLVKVSEFNLRYLSVVQIGCALCEENYIFDFTLGLCLKQIPDIENCQRSYINLQSQEQCVSSMNDDFSIAPEISNCQQYIENCNLCSINIEKEIKCVVCQDKYIIENNKCYQNEELNMEDNLFQNQTNKIQSFILQFVPYLKQQVYNTFLNSLNAIQKQTCDPECILCHQTGSYCKICPLNYYKKYIITEKSKTCTYCHPLCEVCLTRSDKDIQLNFPNFIVNQENYIYTKKCIKPYQDPSIYYDQYNEIVRYCFNQDCKDKFIFDISYISCDFTRFNRMYESMINTQYCNQIGIQSLTVNFTFLVSRKSCFLFIPIDFQTLLKQKVLTLKRVDFILQSKQQLEITLATNNTFRNFDQIEISNLSFVIDADQFFIFQNGNNKIDLILSNFTINQSLIQNIDSLFISEIFGKIILNNVTIQNTKFINSSLFNFQSYQVQGIIQITNLQILNCTFIESALFKISKIQSIISLTNFILDQSLLRNSSIFSFFISYSQQQRYLNAFNTIIKNNNFSHSYLINCTNQIEVSISDFQFYLNFLEASVILSISNKISMIQINIYQNKYSFSQFLSITQILLKNQLICSIYNFKANQNHFQASNIILIFSTFSTNSLIIHFEDFTIIENYKSSKQNENIQLFCVNSQEIHISHFLIVDNHDQVIFYLSENTYISITNITYQNSILDFKIPLSFNCLITNKTNKLLYIFGFTSIQITNIQIFNILSIDEPIIQINPSQQNLSYVTRQIKIINVTFTENILIQSSLINLISLLIIESDKMANILLQNIQYEENFLHSYTSTAIREIASLLYISSSLSKLKIVNFICKNNAFTNSSNPFIAIMSADITLFNYTINNHNYLSQQLWTKYYELQFNQSLNQQNLNEIIFQILQIKNIGGAGQFSVQSMSCINCTFSKILSMQSSIFDITTTERGQIQLFNIIINQIENNLLSIEKGSGCISIYSSNSALNLNVVNALFTNIFNRIAPTIFTIIPSKSSNIILLKNIQITNCISLLNQIINVQFSSSKAKENIITIENMRILQNQEIWLNYFLKIRDLLIQEITDTINNQNSMISFQNCIIYIYDFVIEGIIINSIFQFTNLPKLQLYNVIIDNIQMLYSFKLIQVTQIQQIQSIIQIEHLIIKNTQLYKNDVSSYQDYVQRMYNIRGCNIIQQYTESNQKDYFNIILNSLQKNTYLDSSLIYLQSISNQNGFYFKDITFEHNYCQQCNKGLFFIQISDFNYIKFQNFNCIYNSIKQFGCLLFSQQIDLISKIQIINSNFLFNNGSQGVAITALRTNLQIKSCKIINNVAESFGGGLYMSVQSSGFNIKKSIIIGNTARIGGGIYLEDDCNINNDNFIESQLQFNNAEEYANNIKEIPNHLAFVINYLENPSKSVLQNNEQINKLDLKIYRMIEQGIEIFAKDLLIPSNQILKTFQIFDIHNSNYQPFIKDIRLYFKNTRNEHMHNLVNSTCTVKDKIIAKDQQELLDRSVNQLLFFDNEKNSFDFGSLTFRLDPYQQNYSHLKIEISCYIKESKKTLKYILDAKSLKCQLGEFYVDNGCQICQSNQGYYSVTYNSTKCSIFDKDKYYNITSNMIQLLPGFWRPNNFSDYVEPCFKNPLFCNGGWQVSDQTCSLGHIGALCEECDIYNIRGYGNYFKNYWDQNCLKCQFQWSNILPFLVGCLWTIISISMSLRSISRSNSLYASLIIAQRFSKILFKLNQDQESIQLKMMINYIWIFSVIFTFNIRFSFSLLFIEQTSDTSYFIAKDFDCQISSIEQIPIVYLKIFTMLIFMIILFIFTVAGSILYSLIIKQKYDLSLLSNTALYLYVFNYAGLIKMFSSLISKRVVSNNYYIQGDVSLKYGSQDHWLWMYSFIIPGLFVIGGLIPILIFILLMVNKTRLEKIKLRKHICYLLNEYKQQRYYWELIKLFMKSIIIFIMTNFETDIVLKASLLGLCLLVYQILATFHQPFIIQKYNTLDLQTSQICSISIFLAITKYICEQNNYVSPSTMLQIFIIGCFIKLCYPFIFGIARSYFKKYQYFFLNKLYFLLNQKVPNFFFTKILMKMLEKQKLREQKLKNHITKLKNHLIYFSKIQLRNSKQIMTGFYSSSQSSRIHSVKSSRIGIEKLYFDPNEQDEFTKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YE62</accession>
    <name>A0A8S1YE62_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PPENT_87.1.T1710022</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="CAJJDO010000171">
        <property type="protein sequence ID" value="CAD8212776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YE62"/>
    <dbReference type="OrthoDB" id="361536at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14297">
        <property type="entry name" value="UBA2_spUBP14_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000026">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016652">
        <property type="entry name" value="Ubiquitinyl_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041432">
        <property type="entry name" value="UBP13_Znf-UBP_var"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF827">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17807">
        <property type="entry name" value="zf-UBP_var"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016308">
        <property type="entry name" value="UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="15">
        <location>
            <begin position="144"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="14">
        <location>
            <begin position="292"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="13">
        <location>
            <begin position="592"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="13">
        <location>
            <begin position="650"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAD8212776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="773" mass="89411" checksum="6D609A425764D7D9" modified="2022-10-12" version="1">MTSICEHINEQHITWSVQGQMIYKDQCTRCFVEAKSENGIQVCLKCFNGGCIEMHSPQHSDQHNHFIVLNIRLYEIEQPQQKVEDINKLVIADIPQLQSETSVICLKCQKILSSPELDGLVQSIKQANSAFQRQAIAQWEHTAQPCEHTLTMEQIPLVEKNLQKCRSCHLSSNLWLCLYCGHVGCGRKMYDGSGGNNHAVDHSKDYQHHLVVKLGTITSDGKGDVYCYKCDDEVIDNFLKEHLANYGIEIEKQVKTEKTIAELTLDANLTLQLSKLIEEGQILDPIFGPEFTGIDNIGNTCYMNSVVQVLFSIPEFQQLYSLDHQFICTALPYNCVLCQISKVNHGLNSGDFSIKKVSKSALDTEGAQKDFYQDGIRLYDFKQLVAKGNQHFLSNHQQDALEYWQLLNSFFQKTEKQFKLQQLSSIFSYDQTTILKCVNCGGHKVNVVKNQEFKVPVEQPTQDQIDLYHIQEKQKYEERIKTMKPEDIKPFQLNDLPEYDTTFEKSLQLLKQGERVDMFCPKCQSLQVFVKQEYFKTFPKYLFVPVIRFVQENWVPKKLNASIKIQQFYDLADFKEPVLQDIDALKEQNVQEYTEDNLQTLMGMGFGENRCKRALIKYKNDVEAAMMFIMESLDDPTQDQPLQQKQQGPQISEEFVEQIVAMGFNQDQARFALSKTDNNLERAMDYIFNHDLELEMKQPQEKQQQKQDQFENQTSKYELFACIVHLGKSVHCGHYVSYIKKNGEWILYNDSKVAKSAEPALNKGYMYIFRRLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YFQ3</accession>
    <name>A0A8S1YFQ3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1660105</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000166">
        <property type="protein sequence ID" value="CAD8212138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YFQ3"/>
    <dbReference type="OrthoDB" id="287523at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8212138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="223" mass="26122" checksum="10EC6C5C60D13390" modified="2022-10-12" version="1">MSKVFSPDQRNLGGRRIILQQMLQHLHHVETAKSTLSQTNSSSKLLRRTPSPVFKFLDQYKEVLNTFDKVQKIQQKRPSVEKFKNNYFLKKVQTTNTEHQKNFEKCKKRIQEMNNKNKTTLNPALFFRRVDKSLINKTCPIMQPRPQKKEIQQDIDWESIAKKIPQLTDPLFKNKITDLIVSNRIYSDDDYAFLVCLISSVNNAEQSKIILIMSQILNELGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YH38</accession>
    <name>A0A8S1YH38_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1690044</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000169">
        <property type="protein sequence ID" value="CAD8212583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YH38"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8212583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="158" mass="18806" checksum="DD0BE56FC737631B" modified="2022-10-12" version="1">MEQALKTLQTQSPTKQMQVVFINKNNLNRKYSPFQIQLNQSSKYQYLTEIERINGNQIISYQKNTQKSIIKKCTKDLQNKQLISMPQQLHQKLLFQKQKDLVQFRKIIKNFIIIEFLQKPELYLCNFNTSKQKYKLDILLLHSTFVKPIANQSTPLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YHJ5</accession>
    <name>A0A8S1YHJ5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T1950007</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000195">
        <property type="protein sequence ID" value="CAD8214076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YHJ5"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8214076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="64" mass="7567" checksum="77B5C9D8E81A1197" modified="2022-10-12" version="1">MSESRIRKAKLSLIMLLKNLTQDSKYKIISFVFVDDLYQQLNFFVKQFINYIRNSNQACLINGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YKU5</accession>
    <name>A0A8S1YKU5_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T2020003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000202">
        <property type="protein sequence ID" value="CAD8214253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="300641at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051561">
        <property type="entry name" value="FRAS1_ECM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45739:SF8">
        <property type="entry name" value="FRAS1-RELATED EXTRACELLULAR MATRIX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45739">
        <property type="entry name" value="MATRIX PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8214253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="1382" mass="157642" checksum="920CE376C2758E10" modified="2022-10-12" version="1">MEQLKQTEYIEYSGSIGINLYKNTQIVYNFIEQLPTLTIIFWVKPQNILSSFQFLSFTDDVLQETSIGFGVNKFYNLLFYQIYNKTPVCNLTQNIWNHVTIGILDISFDDDFEQLNQRKLMRIFIDNVQSNLTVITNIKPYKRLVLGPVFMDDKGSEIIDIQDIKIFKGFGIRQTLNHDCLIFVGDYCAFCKSGTHYCKEQDPNDDTKVYPCPAGYKEQQNSCSKIIIPNCLRLSSSSLSCDICEQNYALMNGQCIQNSVILSSPFKCSDQNAQFCSFNQTLNVLSNTLDEKQVSKQISKLCTNNLYSSSQLKCTGAAVSSCLKSQFNSKCTQCNNNNVLTELKECKSSCSTNLRFIASNKICVGKCERYLFNLTFPNGKQNTNYYCNPAINCTVTELDIGYYCLTKEQFQTPQKITYLKVNTLTTTKCHSKCYYCFGPQESNCLKCFQNFYYNPYMTSCIPDCSITQNNKFNFNNKYNWTCELECPTWLYTENKNCVKECSVGYKLFQNQCLKANLIDTYEVVQEIPDEVSPKSIVLLQYCPQFCKSCTSDKFCTSCLYQYPMDQNMCVKSCYPQYLEIIDNVSSCRTSCDPQDLIYENNNINGYQINQCFKEKCGTIRANKDYQTFLHQTDTHRCMYPCEDGYYGNTLLLQCLPCSHTCATCEQSAIFCTSCPYFKFLDQNNCYDSCQGKFQNYLDFVCEGSCSSGYQVIDQSLSIYACVQSCGSKYQTFQYSLNNRCYEEMPEVGAYCEGFNCYNCNYKCKTCSGPNSNQCLSCKPKSYLKNNECIKQCYPQFNDSLNWKCVDVCEQSIKTFGRENINGNSVIVTYCSVTCLFKQYQFKEICYELKPSETYCLDRKDYYFCDSCASVCKECQNAYSTSCSKCSPGAYIHGSTCYTDCPSIAPYKDQITLQCVSSCTYFAENGSCVYQCSNSYYQYPALRTCYQMGCPMGTYNLQYTKLCLSCYPGCATCKNGNYNGCLTCSVGYFMNGGTTCINSCIIEPDLVQDWVNGKCVQQCPQGTYIRTLDSGALACTEDCPKYYYSNICVSACPSQTYLDKQICKSCAGPCSECFGVKINECTKCDLGYYRDQTTCYSVCPDYKPYANLADQTCVAACPDYLYLKKNYCFSPCPTFLYYYELNGKKECVDQCYSKSYLWLGKCYECNSICKECFGPNNGNCFECELPYFLNDQTCQNNCPQFYDLTDHVCKDACPVHLVIQGVNCQNECDSGYLVYNQVCVQQCPKFAYLVDDHCYDCNLFCSSCYGPTANECYSCIENYFLDEQSCKSVCPLYYNNETNKCMADCSNMFEITEKKECVSSCPSNYIICQSKCVLVLQDGYYLDGNQCQQCDSKCSKCTSATVCQACANNFYLVSQFLCQFLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1YMG3</accession>
    <name>A0A8S1YMG3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T2260003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000226">
        <property type="protein sequence ID" value="CAD8214571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1YMG3"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8214571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="103" mass="12216" checksum="ABC976E28BAF5F46" modified="2022-10-12" version="1">MSQLNGSLIMRTAYLQTRFIAQKLNSSSRIEQNQMQTSQKAKFNNPKQKKSQINFRFDLKNFEFSEVLNQQNYLGEKVFGEIQVSGRMIYSYEIAKRYIHLPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RVK8</accession>
    <name>A0A8S1RVK8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0010047</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8131450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RVK8"/>
    <dbReference type="OrthoDB" id="285047at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053083">
        <property type="entry name" value="TF_kinase-domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44305">
        <property type="entry name" value="SI:DKEY-192D15.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44305:SF24">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE C03B1.5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="37"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="352"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8131450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="412" mass="48283" checksum="F802349A48A324C2" modified="2022-10-12" version="1">MYPEYCVAQRQQPYPLHKITQSYNPVVQNEPRPRQPNENPKDYMITAYTSINHVIDISPTSSIYRLDPFAKYLCCWTNAVGPEEVSAIQERTKWKHPNLVSFLFFEEQSDKFSQSRQLKIFYEYLPLDIKQLIEKRSKTQEYVPEEEIWKMISGLCEAFVFLQKRGITHSALTLESVYFDEEYLLYRIQDVSPFRARAPHLFENQFKSPESEGNLRVNRFKQDVFGLGMIILSLCLLKNCDYLFETGGISVISLNDCFDQLRTLYPGRVDQILKKMLLIDFNQRPDWLEMQQLLGNLKDMQFTNLDLGLSNKSSILNSSFQLQQQLQQQQQQQAYKAYDQITLSQENREIVNNNNNNNNNNNNNNNNNNNKFTSSINNNTTIKTTDSVDQKLINLNKRIQETLSKSLNKNIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RWU4</accession>
    <name>A0A8S1RWU4_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0010662</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RWU4"/>
    <dbReference type="OrthoDB" id="285925at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8132538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="101" mass="11809" checksum="8BD882AE93084B42" modified="2022-10-12" version="1">MSKITKEEAYKLKKLFFKSQNILDKYKPKKYTNISSRLPSLQTCQSPKTVLPMKQCVTQSIKELLNANVYQYVEGILYTEDCVMPLKKQAQKVKKIVRFVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1RXG1</accession>
    <name>A0A8S1RXG1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0010453</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000001">
        <property type="protein sequence ID" value="CAD8132122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1RXG1"/>
    <dbReference type="OrthoDB" id="294773at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8132122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="202" mass="23066" checksum="68B591868138DCA8" modified="2022-10-12" version="1">MSRYVNLQDKSVPCKPLEEFDIKIYREKLNDIYGKSPLASQYTQPSSPKKNNKIVNPSFDLLQTKLKDFSNKKSASPLKLKVDQGSPNRASGMQQISKIGTLNFTLPSKQMRVGSVYNNQQFNGPISEDRKIYPIRFETEVQEKSSYTQKPITSMIPLHEIMNMRNKLENAQLNVNQISSAYTSEMVKLASVINSQLKKKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S1K0</accession>
    <name>A0A8S1S1K0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0020349</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S1K0"/>
    <dbReference type="OrthoDB" id="292030at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8133330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="184" mass="21664" checksum="2C85B1F5AF60F18E" modified="2022-10-12" version="1">MNIDQGIKQFNGDERQYKTSLITFDSLTLCPSINNIAEFYQNKDLKSIYLELKQIYQSAQIVNLTAFGITVSQFMQFFERVNKQEPNYDYQLHNQLRNYFIKVKETRELISQYTGVQLDCTQIDFILQQMLLGQNLNNTLSQPKSNRSNENPTNLYDEKDQYLQTKDNAISQPRKPNEDCCFIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S1T0</accession>
    <name>A0A8S1S1T0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0040155</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000004">
        <property type="protein sequence ID" value="CAD8135301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S1T0"/>
    <dbReference type="OrthoDB" id="307226at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="94"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8135301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="321" mass="39432" checksum="E8A47A842EADEBDA" modified="2022-10-12" version="1">MYRNSNYSFQEPKQKEIDFQAVDRLLEYTKQTHQNSQRQIQQPDYDEKEKYRNVRPNSLTQRISLYESNQQQSRRERLSFQDIDFCKIVMFLMATEIERLREQLEENINQEQIRKNFEGMQSSINQWRLKFERKEEECDQLREELMNLKQINKENYEKNAQVLYLQIEKLNNEIQEALINSKQKQDEINSLIQQLQQKKSQLDQSDITIKQLKNEVQNLQNKHDIKYDQFIDELNNEIENLQQNYRKLYNDYQQYKQNQSQMYEQQTQVPYSRIQSQQQYILQENQEINMLNLMIQQLQNENAKLKDDLFKQSRRTETWCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S3C2</accession>
    <name>A0A8S1S3C2_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0020366</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000002">
        <property type="protein sequence ID" value="CAD8133364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S3C2"/>
    <dbReference type="OrthoDB" id="312981at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8133364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="268" mass="31471" checksum="A21223A328EA616B" modified="2022-10-12" version="1">MEVIIEMSGLDQEESFKTILDDNIELINIGEMIQEAAYYQAFNLSEQFCLQIKDTNKFIGGRSTIIKICLNQNQINPQVLVFHKGLDGQMKMNKGEIIDINFHRQPQTINKEIHNEPQIHQQAQPIVEDIIKKQNNDQQPKGIVIGGDNLTQSQYIKKLEEDLNKANQVNNIDEYDQVGTSNNHLNYHQSQQISYIENYQQSQQLEIQQSSSQMEINNQQNNLQHESLIETQIKKQQRQCTITIWWDPQEQQKYEHLFQQDKIKYKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S489</accession>
    <name>A0A8S1S489_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RCC1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0030128</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S489"/>
    <dbReference type="OrthoDB" id="10256179at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051553">
        <property type="entry name" value="Ran_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982:SF1">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="9"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="RCC1-like" evidence="4">
        <location>
            <begin position="10"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="66"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="118"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="226"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="379"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="449"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="461"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8134090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="493" mass="55554" checksum="419A2BD8DE285D46" modified="2022-10-12" version="1">MQSEQLSEQKLYAWGSSECDQFLAKDEDDEELYEVKRPYQIKELSNLSIVQVACGGMHALILTSQGKVYSFGCDDKGVLGRPQIEGQDARLPDLITDLPPVNMIACGSSHSIAANSHGLVYFWGFYANTHGPIGEAVSKPKKLDNITNGIKKILCGQNHTMVLLDNFKLLTWGDAETLVIGRKSETRRSVIQNLNPQPIKMKRPIIDIFTGASHAFVKVLLKDNKPGIYGWGLNNYGQLGIGNQENQQLPYLVEFFENMDIVDMVGGEHHTIALDCEGNVYSFGRNDDGQLGLGEQLNEMLRIEAEKQKVLEEELYQQQQVNKKKTSKKNKQGDLFEVQKSQKVIGYEFITTPQKINNIPKMQSIFGSMHFNFAISQQKQIFSWGNGQSYVLGTRNENSQFQPKDVTTIFKNEKLLQISLGASHVIALTSSDHTFELPMVDQQVLQINDPKSKLKKGRKSNSREMSKEKSISMKRSVQKFDDSIIPVKDLRID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S4B7</accession>
    <name>A0A8S1S4B7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0030277</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8134382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S4B7"/>
    <dbReference type="OrthoDB" id="296009at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8134382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="399" mass="47963" checksum="2C57F48DB32D8604" modified="2022-10-12" version="1">MQYYKSLSSSMTQEQKFGELVKSINNTSNNICDDEQSQKFYSLINQSIQIQYELIQNQEASKLSNLQLLKISNFQFIKCHECYFCIIFNLSNDKLQYRLKNEIMKYSFQTHFFDFTLENVNKFNAELEAFDNYSNQNFRECRIIIFSKDFDQKIIEKVNSFPYIYFCGDPTNVNEIYLENLQLHQIFLTLDFQYKGILQLLPTGLSILNQIIYTNIKINGIFQDINDKIQQIQHPSLKFEKSEDNIFLFYNIPYMNVDEYLYIPLSLSINKIEISDIFQKNEELKYTKDINNEVYDYVNKLKILNQINNQIKFSELEFSIILKDQFFSVDQLMFRNQIKQIKEFQKENEINEGELNEIEKVLEDLCQCNKPYFQKLITFFQMKQSMLYRMKINSFIFQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S4U6</accession>
    <name>A0A8S1S4U6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0030064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000003">
        <property type="protein sequence ID" value="CAD8133964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S4U6"/>
    <dbReference type="OrthoDB" id="306416at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="108"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8133964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="233" mass="27237" checksum="F0530BD9B77D8D38" modified="2022-10-12" version="1">MEEKKYKATETIIKQGDDGDQLFLVDQGLLDCKQRNKQNEEKIVKSYQPGDFFGEMALLYNEKRAATIMAFTDCVLWVLDKETFDTFIKQPVLEKRQRYDGILQKFPILQQIDPYLRQQIADALHVVSFKAEDVVFKQKDKGDYFYLIQEGQLKAFKKGEEDEDEEQCVFEFCEFDYFGELAMLKEIHRQATIVCETDCILLGLDKQSFTKLLAPIHDVIKQGTGRYLNFSFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1S7W0</accession>
    <name>A0A8S1S7W0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">rRNA adenine N(6)-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0050094</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDO010000005">
        <property type="protein sequence ID" value="CAD8136326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1S7W0"/>
    <dbReference type="OrthoDB" id="74991at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000179">
        <property type="term" value="F:rRNA (adenine-N6,N6-)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727:SF7">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00650">
        <property type="entry name" value="rADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51689">
        <property type="entry name" value="SAM_RNA_A_N6_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA adenine methylase transferase N-terminal" evidence="6">
        <location>
            <begin position="4"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="1"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8136326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="159" mass="18688" checksum="59B687F2495A8D1F" modified="2022-10-12" version="1">MKNTVSKSICVEIDPRIIIEWTKRFKYSQYSDKFKLIQGDFLTAEQPFFDLCVANVPYQISSPLVFKLLAQWPFWRYAVLMFQQEFAFRLVAKPGNELYCRLSANVQMLSRVDHLMKVGKNNFKPPTKVESSVVRIEPKNPIPNINYIEWDGLLRICFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SC84</accession>
    <name>A0A8S1SC84_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0070234</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000007">
        <property type="protein sequence ID" value="CAD8138651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SC84"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000766">
        <property type="entry name" value="CAMK/CDPK protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001502">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="25"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8138651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="414" mass="48590" checksum="FEE5EFC526EF1752" modified="2022-10-12" version="1">MKHIKKVDLIKNSSKNKKFQDIYEFNKNDKLGQGSYGMVYRAIHRATGLARAVKIIHKASVKQKERLTNELRTVELLDHPHVIRVFETYEDNDYIYVVMEICKGGDIFDKVLELGNFDEQGALIIFIQIIRSVVYYQSLNIVHRDLKPENFLFQKNNDLNTLYIIDFGLARIFQPGILQQWTKAGTAYYVAPEVLEGTYDNKCDLWSVGVILYVLLCGYPPFYGETEQEILYSIQKGKYDFDGPEWKNVSLQVKELISQLLKPSQYRLNLEQILKHDWLQKYVQKGEVILTKANIERWQTYHQIKQISLLYLATQLEQTEIINIKNGFLFMNKSQSGILTKGEIEDLIKYKYQDLEYYQFIAICLEPTVYKIEKYLILMFTYLSSNGRITKDSLKAVDINLECNLDYQQFINLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SDP0</accession>
    <name>A0A8S1SDP0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0060439</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8138078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SDP0"/>
    <dbReference type="OrthoDB" id="10359088at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8138078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="62" mass="7560" checksum="7EC80618145BA2AC" modified="2022-10-12" version="1">MRLSFEHCQIILVRKTHISDIQIYLQEQNTDLIFRFTKDIMPFQRGLFHSRTFEFTCPILNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SDV6</accession>
    <name>A0A8S1SDV6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphoribulokinase/uridine kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PPENT_87.1.T0060360</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000006">
        <property type="protein sequence ID" value="CAD8137920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SDV6"/>
    <dbReference type="OrthoDB" id="106623at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02023">
        <property type="entry name" value="UMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001802">
        <property type="entry name" value="Uridine-cytidine kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000764">
        <property type="entry name" value="Uridine_kinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004018">
        <property type="entry name" value="PRK05480.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="5">
        <location>
            <begin position="27"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAD8137920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="462" mass="53970" checksum="E7A2249BD4936632" modified="2022-10-12" version="1">MINDSHQMKSPINLRKNSEYEPQRILVGVTGGSAAGKTTLCETIKREIQYDSDVDMTILSCDSFYKGIDKSKIDISSYNFDHPDSLDWDNAFEVISTLLSGRPASVPIYCFVNHKRLDQCSIVLPAQVIVFEGIMALYDERIRELMSYKIFIHCDDDIRLCRRILRDVKERGRTVENVLFQYSKFVKRSFENFISPLMSQADLIIPGHRQNHVSVAFIVNHLKNMAKQNGLLREKLNTLLYFGDLLYSIDGFMRNRTKVDFSNPNATFKQLFFPEENMKPDFLYITQNFLAGKLVNDKQIYKVVKSCFKQTLFMLEKCIQQDKYEIDQFQCVHLNEILSNNLKLNENTKFIVLALPLLFKNGLVKLHQIEDNIKQYQSVFIKFYQVQLIVVNVFSDIKSITDINWTFKKLKIYINAFLVGKLDKFVQIMNLPIKSEPELFYGQDKFFLKLNRFITKRQGKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SJI1</accession>
    <name>A0A8S1SJI1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PPENT_87.1.T0080138</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8139517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SJI1"/>
    <dbReference type="OrthoDB" id="290094at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000315">
        <property type="entry name" value="Calcium-dependent protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PH" evidence="16">
        <location>
            <begin position="32"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="137"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="476"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="519"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="479"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="519"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAD8139517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="554" mass="64597" checksum="FC38D670BA56AF30" modified="2022-10-12" version="1">MQTEQFFFDSSNTNDKNQWVKLDSIKRPISTIRLFEGTLFMKSKKQDAFKPKFFKLFSDRLNIYKNGREQKEIAALFLTNVYLDLKLLIEYEKDKFPIILLNGNRKLVLNARSQESQTKWIEQFKKTCILNNYRGVYTNIKVLGKGTFAKVLLAEKIVNKSKFAVKTFQKIALMDKNNTQRQGLLNEINLLRSCDHPNIIKLYEIYESGDYIYLVMELLEGGELFDLILETPQFLESKIALIMFKIFDALEYLHTKNIMHRDIKPENILLNDKSDNFDLKIADFGLASYTESELLIKRCGTPGYVAPEILQDQKYNEKVDVFSAGIIFYILLTGQAPFYGNSLDDVIEKNRACQINFKDLKVSKDALDLLYKTLESNPKNRISSLEALSHPFISSLYKRQINSMEDIPLDDLPNDNYSAFENMKKFCQNNMRFKMSKLKKNELVQQTPFMGTREYDPIKASCDSWLNEKESKQIIRSPQFQQEQSSDSEFSILSTPESRKDTQKFIQLNPLQQIAYKQSPNQSIYSPNNQQKEQIQMNFKKASQVRDQLKKLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SK26</accession>
    <name>A0A8S1SK26_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BART domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0080502</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8140200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SK26"/>
    <dbReference type="OrthoDB" id="433414at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097546">
        <property type="term" value="C:ciliary base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023379">
        <property type="entry name" value="BART_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038888">
        <property type="entry name" value="CFAP36"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21532:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21532">
        <property type="entry name" value="PHOSPHODIESTERASE HL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11527">
        <property type="entry name" value="ARL2_Bind_BART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BART" evidence="3">
        <location>
            <begin position="38"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="268"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="374"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="165"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="309"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="411"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="283"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="374"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8140200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="440" mass="52138" checksum="93C59EC9F633FB0D" modified="2022-10-12" version="1">MGPKSSKDKPQEPQKQPPKPAQIKSQNVKKKPSQVDEDIWMYDLILQYLASPIWRNPISDFLDENCIIFDDEEENKIVYQEIHKKFKSMIEAMIEQLMVDIGVEDEEQLRKVIEIGLKNKKHKKYFEQLLIVENFLVFKKLMLKRNKELEYEALKELQKIDGGKNPEMEMKVKQAELEKERMEIEHAVAMSIAVENEKAKIQIQEEEEFIEAIRQSQISYQRQQEEILLSQVQQQPAVVNHQAPIQNQQIQPQPDQKPESVLIFEDDVPKEKEQAPQQQQQVKQKEKTEPSQIKQKEPLPQIVQQKVEVMSLAQEREALLAQGNQLKENLKEIEETFKQNIKNQQDQETLEQRKQRLQQQRELIKQKKIKERQDELKKYQEGEQQEQKPEQQEKGPLVVDMTEFIADQKRSQELELKKQELKKRAELLKKIKQDALQEQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SKS4</accession>
    <name>A0A8S1SKS4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RING-CH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0080415</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000008">
        <property type="protein sequence ID" value="CAD8140026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SKS4"/>
    <dbReference type="OrthoDB" id="288223at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16495">
        <property type="entry name" value="RING_CH-C4HC3_MARCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46065">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCH 2/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46065:SF3">
        <property type="entry name" value="FI20425P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="244"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="11">
        <location>
            <begin position="130"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8140026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="334" mass="39357" checksum="490B05E755AD09DB" modified="2022-10-12" version="1">MQTQIHSQDAKISQNLIYYYYNYLIQMLIKIRPKYKNTTSQTFQNSINVSKSQICPQPDDIIQDFNELGLNVYHPSNNIKNQQNQIDEQMIIVENSQKKQIIVNLNKKTIKKVKRYIETPQIVLERIKNTQIDLNPTCRICLEQDSLDKLISPCNCDGTIKYIHMDCLKTWLLKIEFQAQCELCNSFLQCQLTFSNKYVCSRLRQMSISQKLFFFIFPMLTLILFALGIYFITQIIKKSKDQYAYIFGVFVCIPAFCMLTYLFITMLIDAIRVKFVKTWTILSQDTRNDTDVSLKPLKGRARSQSLPQIYPEKTVQEQHFGKTIFKYITRQQMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SKT0</accession>
    <name>A0A8S1SKT0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF5745 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0090110</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000009">
        <property type="protein sequence ID" value="CAD8140508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SKT0"/>
    <dbReference type="OrthoDB" id="308328at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044039">
        <property type="entry name" value="DUF5745"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19016">
        <property type="entry name" value="DUF5745"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5745" evidence="2">
        <location>
            <begin position="78"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8140508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="545" mass="65730" checksum="99AC95AA5D8B2496" modified="2022-10-12" version="1">MNPKVQQIIEIYKQIVQNLKLEPKKIKNEENYCIIFQILFPFLEDEIENIKNQRIQAGEKINALIEFLVIIQIEYSQSNQVLNTNLSHISGPKIVQGDLKHISNFLQILQELSKLYKDKVLNPNINSQQDYLANDSNSQPSEYVEYFDNSQDEQQIYHEEENDLLQKPNKQQIQYQVINSKQRNNKSLDKYSQKKEDRKSSQVKYEQEINQQKRPIRSHSFQKTSSNRSKSQQKKPRVFTKEKYSENTQSSYDQQHTFDFDTLNISEIDYKFLQQYDEKDIEQLDENDPIKKKYIIEKKKEDVLKILQEKILQEEKNQNCPVIDNQLDIQIQNIKQCLKKLKPKPITEDIENINEQKNEYKNFQQVQLQNLQHNKSIQNQQQQNNHPRISTKIKQKDSLRNQFENVHLVMQLKLRDEKLNYLKKIHRIVFELEKRQVIDEKNDQKQARRQQNILARNILDQYESSFDDQIIFLKDKIQKQRKDRLYEGISQKSILSKLEKELKEEKIKDIQKQKEIWKYDKEKFEQLMKDDGELEKKILQIYKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SNF0</accession>
    <name>A0A8S1SNF0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0110042</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000011">
        <property type="protein sequence ID" value="CAD8142245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SNF0"/>
    <dbReference type="OrthoDB" id="6612291at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF12">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="125"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="275"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8142245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="311" mass="36840" checksum="469F0EF124E03B79" modified="2022-10-12" version="1">MTQPLLKNTTRWRKIFICYFLGLGYIEDSLNGSDSNNWKLIFAFPALLLFCRSCILKFRYKMDSPITLIKRDKYLLAKQILRKIYQLQYEDQAFLECKLRVQKKSDQEKGYSVFSLKSIEQQQQLAAYFVFVYTWSGLFALFSYSLQIFSEMSKDDTTLNAMFNLIIGIVQFAPALISKYVYGRWDKRSLLLFRLTVLILCQILIIGLSYSEERYLIYFHLYLFILICIEFGTNYLGNSMILEINSSEGTYFCFVTLYAWKLLFSYIFQLMFDTLALSGSFIFFLNLTLLSTTFFYFLLKKQKDQHIKKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SUH6</accession>
    <name>A0A8S1SUH6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glutamine cyclotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0120320</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SUH6"/>
    <dbReference type="OrthoDB" id="409395at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016603">
        <property type="term" value="F:glutaminyl-peptide cyclotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007788">
        <property type="entry name" value="QCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31270">
        <property type="entry name" value="GLUTAMINYL-PEPTIDE CYCLOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31270:SF1">
        <property type="entry name" value="GLUTAMINYL-PEPTIDE CYCLOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05096">
        <property type="entry name" value="Glu_cyclase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035714416" description="Glutamine cyclotransferase" evidence="1">
        <location>
            <begin position="17"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8143620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="262" mass="30671" checksum="3F9C1C3576B946EA" modified="2022-10-12" version="1">MIALVIIITLAHSYFGFEQQFLQTQLTGKFKYLNRVLRDNKRNPQYTQGFTFLNDDTILESVGLYKKSGIHYIKLNDVNTFQHNQNLDDQFFGEGCDIINNKVYQLTWLERKIFVYEKEDLQYIGQINLHPSIKEGWGLTHRVFNGETQILISDGSGKIHILNEDFQLLKSIQIFYGIIPVNYLNELEYANGILYANEYRKTHIFAIDLEKGKVLAKYNFPDLVKEANRPDGCLNGIAFNQKTQTFWITGKNWPFIQQVQFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SWS7</accession>
    <name>A0A8S1SWS7_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein disulfide-isomerase</fullName>
            <ecNumber evidence="4">5.3.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PPENT_87.1.T0120395</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Catalyzes the rearrangement of -S-S- bonds in proteins.</text>
            <dbReference type="EC" id="5.3.4.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.4.1" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDO010000012">
        <property type="protein sequence ID" value="CAD8143769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SWS7"/>
    <dbReference type="OrthoDB" id="2121326at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03001">
        <property type="entry name" value="PDI_a_P5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000032">
        <property type="entry name" value="Protein disulfide-isomerase A6 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005788">
        <property type="entry name" value="PDI_thioredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057305">
        <property type="entry name" value="Thioredox_PDIA6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01126">
        <property type="entry name" value="pdi_dom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45815">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45815:SF3">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24541">
        <property type="entry name" value="Thioredox_PDIA6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035776356" description="protein disulfide-isomerase" evidence="13">
        <location>
            <begin position="18"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="14">
        <location>
            <begin position="7"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="14">
        <location>
            <begin position="143"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="134"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="135"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAD8143769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="425" mass="47299" checksum="BD20B2CB5CD7B064" modified="2022-10-12" version="1">MNKVICLLSLLLATSYALYDSRSKVQLLTPQTFREKVLNSKSLWIVEFFAPWCGHCKALAPEYEKAAKALEGIVNIAAVDADAHKDLGGQYGIQGFPTIKFFGENKNSPSDYQGERSAQGIINFALEQVKSTVNSRQKGSSSNRNQQKQSSGSGSGSGSGSADDVIVLTDSTFDENVLKSKDSWFVEFYAPWCGHCKKLEPEWNKVGSELKGKVKVAKIDATANTQLATRFGVSGYPTLKFFPAGFSNDSEAIAYDGARDSSAMIEFALEQSNKSKKVEVVELLSKDILKENCLDYNGVCIIAFLPHIYDSNKQERNQYINQLLEVAKSLKNKPVNFLWTQGGDNYEFEEAFQSAAAHPSVMALSGRKSVYAKLKGAFSKQNIEQFVNNVLNGREHFNQYSQLPNFKKVEKWDGQDHKPVYNDEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SXP8</accession>
    <name>A0A8S1SXP8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CTLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0150341</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000015">
        <property type="protein sequence ID" value="CAD8146431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SXP8"/>
    <dbReference type="OrthoDB" id="972532at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051350">
        <property type="entry name" value="WD_repeat-ST_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22838">
        <property type="entry name" value="WD REPEAT PROTEIN 26-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22838:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CTLH" evidence="3">
        <location>
            <begin position="36"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8146431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="553" mass="65442" checksum="CBFF5CEE3FF631C6" modified="2022-10-12" version="1">MNNKEEKIKLILQFLRQLKYDNTFQKLQEQSQIQLDPQNLELITQSIKNYDIIQLEQILNQYVDKETKKKCILKLLEQKYIKLLKLHNVQEAIQLLRNHITNFCSDEEQKHQYASMIYLPELKVKQEQELIDEIIQLCYKQIGLIEPNRLVTILQQQQANQILDCQFHIKLEQNYNIFKKHTCNSQLNIIKQQKNISFCEFSINGQYQALVHGLQIYLYQFDNTKNEYQQKPRKIQTGQTIAITSIIFSPCSKYIGTSSQDFTVFVYNIQTENKYRLEGHNAIVQCLNFVLCDQSIKKQKNEYDIYTISIDGWLYEWNENERKGGLKIEEKLLKLHTHQQKELMLLTSQNKISLYKLYTKYQISQTSSSNNNQNSIVDKHFNFVLILVNDTIQELYLYAIPDLQLIKVFQTTSRINASLINQFDIGWLNYNLIAAGTNKGDLLVWHIQKSSKPIESYQISEYDKEISCIKFHPTKNQLYVYLKKDSRKQLSTQQQTTEDLRTQLLQQRNQYQRQSGGMQSLLNLLQIIARQDQMNSNRQSMLQEDKESSDEDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1SXV1</accession>
    <name>A0A8S1SXV1_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0140023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1SXV1"/>
    <dbReference type="OrthoDB" id="292797at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8145000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="385" mass="45754" checksum="3B2A0926AF8B07BF" modified="2022-10-12" version="1">MNSKLIRQSKSTQKIPQQVSQQKNLEQVYIKRLQEQEAFMPKDIYIQKHKSMHQIMNNQQTQPLRYYSHINEIRSIKEFIYISEKLKQKNIERKMQERLELKGKLNQIKQIEIGKKLQYYIERKIRLFIKDRQHEENPYNKSFEAFYEFYSNDPKLKTFEKDKLLSEFNRLSSQVLNVPVSRSQHAKITISSPPKNIYQNNEDNEPTQNEEDDGTSKFLTMKAANRLLGLMMVESSKGKRTPIVWALNKKKELQELLNKPFGGKMNFTMNAFNWLIDNAIQKSQVYINKNQIVEQKSVDPKKISELIDKGLVNIKRTLSTRPASSNNLRSCQQLKHRNDVAQEYCISERVRQNVIQQKMRNQKLQEQQKIQKSYLSNDSYVNHYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T1F4</accession>
    <name>A0A8S1T1F4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MORN repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0140283</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000014">
        <property type="protein sequence ID" value="CAD8145518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T1F4"/>
    <dbReference type="OrthoDB" id="270720at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="23"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="23"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8145518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="345" mass="39307" checksum="F220800245FCD1C6" modified="2022-10-12" version="1">MGQCNNCQSCQKPDGRELTVDQQAYRTSQQQSQTSKQRVSNISGDHGSKSKKIKAAITIQAHWRGYIVRKKIQKKSIGTNDIASAPPIANKYFSSDKDYAQQHSNSKTREQRPPFKYKSGAIYEGEWIGNSRDGQGTQLWNDGAKFVGQWKNNMAHGKGKFYHVDGDIFEGTWLEDKACGFGIYSHANGAKYEGEWLNDLQHGFGVETWADGSKYEGQYNQGKKHGKGKYIWNDGSFYDGDWDNNQISGKGVYQWSDGRRYEGEWLNNNMHGQGHYTWQDGRSYKGGYINDKKHGYGIYTWADGRKYEGEWVSGKQHGRGQYILLDGSIKKGEWIEGKKIKNIQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T488</accession>
    <name>A0A8S1T488_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0160273</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T488"/>
    <dbReference type="OrthoDB" id="288205at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8147230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="475" mass="56843" checksum="7942B30EE605A45F" modified="2022-10-12" version="1">MLQNISQKREQFIIGLKKKAREETFRKNRFPQSKDIIPFVSNNIILQQPYYEAFERGNQQKINFMNDNIEIDETLADCIFKVQQLTNYYNEYDCEPEYDTTKDVERIFKVFNIGYPQIDKSCLHILLAISAGNHQQVQPIHEHIQMLAGYYNSTYYTELQQYILDIFANIACDCYQCRDLILVLQVPKCLWNYTLQCKQKCLFDDFYSLIINLDRMKPEINKFKLIELHDILQDLYIQLYNAKEKLWILKILNRIYSYDKSKLDLSFLNYLLKGDYDEIQLCGEIFKYFQYVSLLDNEITLNFQSQMAHTIIKTINKYIDQQNILRGELKLLIKKGFVTLSNFITDDPQIINEFWEEILPYIQNIPSIFLEVDDFFLFLHNVIKFSKCEDLIKLNKDYKLIKQITQELNDFCEERGNQFLYNILSILYRLISLDQANMKIFNEEDCESRILFVQKMKVSDENYELANMILDKLDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T4Z9</accession>
    <name>A0A8S1T4Z9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0160001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8146686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T4Z9"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8146686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="64" mass="7881" checksum="5B898BA8B68ACFA6" modified="2022-10-12" version="1">MGKKFHPLTKNIKIFLLNLKHHFFRTILNQSLIIHILLYLHLQYWKHKVLLSYKENLQTIKEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T704</accession>
    <name>A0A8S1T704_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PPENT_87.1.T0160368</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CAJJDO010000016">
        <property type="protein sequence ID" value="CAD8147407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T704"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000315">
        <property type="entry name" value="Calcium-dependent protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000945">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="123"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="443"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAD8147407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="479" mass="55233" checksum="DFC2B91BAFE49328" modified="2022-10-12" version="1">MDVIKKDSFFDDPDTTGFWNVTDFPSKDAALNMHDTMYLEQDSELYKGTLGLSNNYLLYFKKQMVKQWINLDNATIEYTKHQKAGLGIKITKNKQAFEFFGDVEPWYNYLKQFCIQRNFSHHYTLLKKIGSGNFADVFKAVNKNDGSEYAIKCFRKAKLNESVDRLAIIKEISIMKKIQHESVIRLYEVFEGDEFLFLVLEYLKGGELHQYMKKSPPFSEEKCSKLIFRLLKAVSSIHQKGILHRDIKPENIMLRNKDDIENICIGDFGLADYYSPSGQYLFTRCGTPGYVAPELLQDKVYDFKVDVYSVGILMFILIAGKSPFDGKDYDDVVMRNYYAKVKFEDCKLSDIGMDLLQGLMNKNPIKRLTAEEALNHPWFASENLTKTCQFKIRKQNQTTLKKLAYFDTNIKQQSAISTQFSPKSSLSSLSPYCVTKCNIEDSPQTPNTPATLRPVNTPRTSAGYKLRQVRRSQIPLKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1T9V3</accession>
    <name>A0A8S1T9V3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0180070</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000018">
        <property type="protein sequence ID" value="CAD8148447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1T9V3"/>
    <dbReference type="OrthoDB" id="340500at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071034">
        <property type="term" value="P:CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22526">
        <property type="entry name" value="KH-I_Rrp40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000112">
        <property type="entry name" value="Exosome complex component RRP40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026699">
        <property type="entry name" value="Exosome_RNA_bind1/RRP40/RRP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049469">
        <property type="entry name" value="RRP40_KH-I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21321:SF1">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21321">
        <property type="entry name" value="PNAS-3 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15985">
        <property type="entry name" value="KH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21262">
        <property type="entry name" value="RRP40_S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="K Homology" evidence="2">
        <location>
            <begin position="158"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8148447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="238" mass="27194" checksum="B108F3C2DE0D36FE" modified="2022-10-12" version="1">MQSALVYITLPGERIPLKSLVIGNTIKTNKQDVNITKSLEIETKHAGWFWIDKQKIAICHNPIYYTPEIEDYVIVQVTGKNSEYYFCDLGNGLIYNLNQMDFEGATKKNKPNIQIGQFIYARVIELNHYLKGKLSCINPQSKKEWTTGENLFRELIGGVIVDLPLNFVQSLLSSQTKPELFKEISKHSSFEVCVGKNGRIWIKGNDAVLIINLLKRCAPLQLEQQLNLINKFASQFQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TA51</accession>
    <name>A0A8S1TA51_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PPENT_87.1.T0190035</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TA51"/>
    <dbReference type="OrthoDB" id="10263328at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058584">
        <property type="entry name" value="IMB1_TNPO1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25574">
        <property type="entry name" value="TPR_IMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="6">
        <location>
            <begin position="23"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAD8149290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="853" mass="97554" checksum="708AC25F369C41B6" modified="2022-10-12" version="1">MQEFVELIQAAFFAKENNQRSQAEQQLVNLKHQLPNDFFQKCSSAFISPQLDSQTRVAAGTLLQRCISYEQGWLTIGLDVKRKIKDELLSQLISSDQNIKKSAASCLSGICAIELPRQEWPEIISILVSNTRHESIEVKKAATITLGYICEALKNQKQSIEKTESEKVLYGICMGLQGEKQIKLISIRALKDSLQFMDQVLAQQQVRDHVTKLLVESAISQDAEIRLASLECLIDYTKAIFDYLEQYILGLWNCTQESIFHKDFAIVAIEIWQSIASEINERSAVSNRTNLGFRKTQKEALQLISQQLIQAVLQNLLISDEDEEDDEEQGIQEAAYKAASSISEALGNLCYQLYLRFIENTLQAQEWQNRKASLLAFSSMVETADVLELFQYSNSAIGEFIKKLADPHKQVRYAAGRVITRIAENYPLVILKHQFADEYINQFSQFLQGNTKLTKYLLWTLVNITEAFRDDPINQFGKYYDFLISQFATIIGRQDFQNGTLLDIAWVGIINCLQCVKEPQKIKTYLEAFGQQMVAVYSQSGWQKEANISGLLSAIHICFLRLVVLGDQCDIQLMNNFYSLIVDYFKKIQTVTQDGFYAISAIAQYSKINFKSLLDDFMQNYLEIGLQKKLEIETFKGAILCLADIARSLQFKEFSKYLSILQYLITCVNDSQVNRVAKIALFVCIADIVLIAEQDAEPYFQAIWQLVNSGFRASIYFTQNKEMTQLEYYESLNDALLNCYLCMLHAFNLNKVPYIPLYQTISELCMFIQATSEKSLTPTVEYIRLCVTCLLDCVAYYKQAKGQDNMSQKILTSPLLISLLDMLKQYSNQQENQQLIMYANDLCKSFQLPQNLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TC74</accession>
    <name>A0A8S1TC74_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0190124</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000019">
        <property type="protein sequence ID" value="CAD8149468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TC74"/>
    <dbReference type="OrthoDB" id="295513at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001233">
        <property type="entry name" value="CAMK family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF30">
        <property type="entry name" value="MATERNAL EMBRYONIC LEUCINE ZIPPER KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="158"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="80"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="84"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8149468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="477" mass="54376" checksum="E0FC2725E30517A0" modified="2022-10-12" version="1">MNQLKKHISVKCINFNTQSTNTRTRSISNQVSKNSTDRSRSVTKLCPAIKLPVPTKNSESVSMSNSKPISKLGKVKIDLGNKATSRSQQTTQKSRSNSQISKEESFPKMLPDFGRMETELLDCQDFMLQLPDIQFPTTEDIIKERIILANRIAYTVKTKKRIPITTPDFYKIALLCDRQGSICKSAKVAMKIIEKSTLKTESAKKRLLLEITLMKILQQYQQFASLFEVFETKKQIYIIMEYVEGGDLMKWTKEKPIPEGQAKNLFSQLIIALQILQSHNILHRDIKLDNILLQGEQIKICDFGVSRQIIKGQKILEQCGTPAYLAPEVIQNKTGYDGFASDIWSSGVLLYILLVGKVPFKGNNMNELNHNIQNGLLNFMEMKKLNLSNDAVDLIKSILNVNPKLRITSTEILNHPWLKELNLKSNKMSTQNANLDLRIISQIEQFGYSREFIIKSLQKQTLCHINAMYSILTKVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TJV3</accession>
    <name>A0A8S1TJV3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0220217</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8151977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TJV3"/>
    <dbReference type="OrthoDB" id="299975at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027916">
        <property type="entry name" value="Kinase-like_dom_ROP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14531">
        <property type="entry name" value="Kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="86"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8151977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="528" mass="61990" checksum="2528DA267B1D1B61" modified="2022-10-12" version="1">MSDNYQLNLPQFPEKSIFSSWFQSSESKEELQEHLKAIQAYLAQINKILIYKPDAINYFVCNTFDPERLKLLTFGKLTKEILYNNYIKSAQIKKSQFNKVFRVSVRQVTLAIHQYLIPQSDFAKAEYENFKRSQLLISDFTYIVKCFEIGHLIKNKSFFNKKIKKTVYNKFLKMVDLQYDTIYAVEEWVEQPMNEVIFLRAQTHNLFQLEVIVEAIITLVSVAQYLQFLQIFQKQFSVSYLFYDENKGFKVGGLSPVLAYKKKYHVQYNQDINDYQALQPPENNTSSIQYGFKNNFNVSSRTDVWQIGIVILSMASLTLPAELISQDQIENKLNLVSFQYGEMLGNLIRNMLIRNPIDRCSLVDVANTAQNLLPMKLEYLKNEEKTEKIQITSISQLQLEELDKYLKEKIKRKYIIHMILNQQVVQQMFLYYLERIKRENVIQLYINLSPQMISDNLIEQMMAIIIEYKNLQQLVINMKGCSISDVAYQYIINQANIITQLKQLTLGISGIQIIQIPETKIRVVVYNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TK65</accession>
    <name>A0A8S1TK65_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0240179</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000024">
        <property type="protein sequence ID" value="CAD8153385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TK65"/>
    <dbReference type="OrthoDB" id="297495at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8153385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="108" mass="13441" checksum="FE9BFB34C21FCACF" modified="2022-10-12" version="1">MFIWEFNVNSYIFQVQVFVLDMLKFIKEMKNYVQKRFYQVQLLFEELQQIYPQCTDWDVILLLDLSNKSFDRAFKLLKESNYFVQYYLQVQSLKNVDRNGLENRWKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TLV9</accession>
    <name>A0A8S1TLV9_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0230224</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000023">
        <property type="protein sequence ID" value="CAD8152754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TLV9"/>
    <dbReference type="OrthoDB" id="303955at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8152754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="133" mass="15696" checksum="9180EE4560BD15EE" modified="2022-10-12" version="1">MNQSAPKRSSSFGKSFNFPFEISSKESNRSIYLTETKTKKIDRSINVPLNSWRELSLIELLNEAIELLQENKSNIIDQQKFKRLITQYENFKQTNEKKQMLFQKRSKIIKSNIENIISSQNDFQNYLQGVKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TLW3</accession>
    <name>A0A8S1TLW3_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Insulin-degrading enzyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PPENT_87.1.T0220238</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the peptidase M16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000022">
        <property type="protein sequence ID" value="CAD8152019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TLW3"/>
    <dbReference type="OrthoDB" id="952271at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000005">
        <property type="entry name" value="nardilysin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000012">
        <property type="entry name" value="Protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000165">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050626">
        <property type="entry name" value="Peptidase_M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032632">
        <property type="entry name" value="Peptidase_M16_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054734">
        <property type="entry name" value="PqqF-like_C_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690:SF18">
        <property type="entry name" value="INSULIN-DEGRADING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690">
        <property type="entry name" value="NARDILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16187">
        <property type="entry name" value="Peptidase_M16_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22456">
        <property type="entry name" value="PqqF-like_C_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="9">
        <location>
            <begin position="24"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="10">
        <location>
            <begin position="179"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 middle/third" evidence="11">
        <location>
            <begin position="364"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="Coenzyme PQQ synthesis protein F-like C-terminal lobe" evidence="12">
        <location>
            <begin position="758"/>
            <end position="857"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD8152019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="942" mass="109853" checksum="ED89257C1E63E0BB" modified="2022-10-12" version="1">MIHKSTIDKRTFKSVTLSNSLLCLLISDPETEKSAAALNVDVGSLEDPVDRMGLAHFCEHMLFMGTDKYPNENEYQQYISKNAGSTNAFTSELNTNFFFSVANQALEGALDRFAQFFISPLFSDSCTEREMKAVDSEYNMNLQNDFWRKFQLFHNASLAGSQYNKFMIGNLKTLQFEDTRARLQEFHKRYYSANVMKLVIYGQQPIEILEGWAQTYFEAIQNKNLLPPSYNVMPFDQTNLGQLIKYVPIKNQDYLELIYIIDNLYPQYRSCPGKYLSHLIGHEGENSLLSLLIKEDLAQELSAGPSNTMKLFSEMSIRIKLTQKGLQQYLKVVQYVQGYIELLKQKGPQEWIFKEIQAIKKLEFDFLEKGDPFNYVCTLASRMQLYPIEDVLRSPYLMEEYQPEQIQKITNQLTGDRLIMFLSSQNYQNQLGNKEEYFGTEYSQTKFLEDITTVFNNAANNISPLLNLPPQNIYIPEHTNVLPLQNGLPQFPQLILQTEQTDLWFKQDDRFQVPKTVIQLRINTIETGYGKLSKTEAIAKIWQALLKNHVREFNYLAEMAKIDASLQLAANGLEISISGFSDSISRFVIGMFQKIISFKPEDYQDLYESTFVKITQELENFKRSQPYQQVHSLISVLLREGSSFETQELLDQLTNITFNDVIHFSNNFLKRCRFEWLIMGNLVKEEAIQIVEKSLDLFKAKTLKYEQVLQIRPVMLNQKEICNYTYDLTEPTETNSGIVVHYQIGKPDLRTQLYNDILQTIMKTPFFAQLRTTEQLGYAVFSLLSDVRGVSGFTFLIQSNAKCPNYVQDRIRTFINENLNKLITEMSEQDFEQFKNSVKVQLQEKDYSLIKESARMWNEVQKHQRLFDRRLQQLNILDDIKLSEVQQYFRTHLIEQTKQFEIHAVSPTHKKDQEDIKSDSLLFSSSEKFKKKQGLYPDYYYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TTM8</accession>
    <name>A0A8S1TTM8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">T-complex protein 1 subunit epsilon</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">CCT-epsilon</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0250311</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000025">
        <property type="protein sequence ID" value="CAD8154316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TTM8"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03339">
        <property type="entry name" value="TCP1_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000003">
        <property type="entry name" value="T-complex protein 1 subunit epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012718">
        <property type="entry name" value="Chap_CCT_epsi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02343">
        <property type="entry name" value="chap_CCT_epsi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8154316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="535" mass="58633" checksum="83C818081EF44141" modified="2022-10-12" version="1">MSLAFDDYGRPYIILRDQGQKKRMKGLEAYKSNILAAKAIANTLQSSLGPKGMDKMLVSPDGDVSVTNDGATIVEKMEIQHPTAKLLVELSQSQDAEIGDGTTGVVVLAGKLLEQALTLLDKGIHPLKISDGFDRACDVAIKHLEQIAEEIDIQANDHEALIQAACTALGSKVVSKRKRELGKIAVTAVLDVADLTRKDVNLDLIKIQTKTGGSVEDTRLISGILIDKDMSHPQMQKEVTDAKICLLTCPFEPPKPKTKHNINISNAEDYNKLYQQEQQYFIDMVKLVKNSGANIALCQWGFDDEANHLLLQNDLPAVRWVSGTDVELIAMATGARIIPRFQEITPEKLGKAGSIKEVQFGTSNERMLVIENCQCTKAVTILIRGGSQMIVDEAKRSIHDAICVIRNLIKDNRIVYGGGSAELACALQVLQYADEVSSVEQYAVRSFADALEGIPSCLADNCGLNPITSVAQAKSRQLSEKNPRIGIDCMDLGTTDMKEQRIYETLSSKKQQIQLATQVVKMILKIDDVIAPDDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1TZ50</accession>
    <name>A0A8S1TZ50_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Calcium-dependent protein kinase 1</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="31" type="ORF">PPENT_87.1.T0290259</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23 33">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22 33">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cell membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Parasitophorous vacuole membrane</location>
            <topology evidence="24">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="31">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="CAJJDO010000029">
        <property type="protein sequence ID" value="CAD8156942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1TZ50"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020002">
        <property type="term" value="C:host cell plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020005">
        <property type="term" value="C:symbiont-containing vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000398">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000199">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000695">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 26" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17" id="KW-0969">Cilium</keyword>
    <keyword evidence="27" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="15" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1032">Host cell membrane</keyword>
    <keyword evidence="16" id="KW-1043">Host membrane</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0519">Myristate</keyword>
    <keyword evidence="12 26" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0564">Palmitate</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="29">
        <location>
            <begin position="216"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="30">
        <location>
            <begin position="513"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="30">
        <location>
            <begin position="551"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="30">
        <location>
            <begin position="587"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="27">
        <location>
            <begin position="58"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="27">
        <location>
            <begin position="535"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="28">
        <location>
            <begin position="136"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00060437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00068067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="EMBL" id="CAD8156942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="650" mass="75021" checksum="B32059134043B62F" modified="2022-10-12" version="1">MGSTCCHTKFQSSSYAELCLEQGSSALILQKQSVSQQEQSQMVAPLPLGKESQTPTKLEAMKQQIQLLQLESIKAGNELIEEQVNTPKGQINLIRKISIESEQQNINNNNKEIRSTNHQETNLLQLSPIQLDSGFNLPQQSPSPLKKNSNRKESLNVEQYNNTTFTSPRIYTTNRQIQDQSASPTKKSIGSMSSLTIRMGVELFVNLKKGSINKVYAFGQVLGQGAFGKVWKVTHKTTGLIRAMKQIKKSELIKEDEQKLFQEMHILKNLDHPHIVKLYELYQDQNNYYMITEYLSGGELFERIKKMQVFTEKRASELIRQILLAINYCHEQKIVHRDLKPENVLFSGPEPDQNLKIIDFGCSRRFNTSKMTKRLGTPYYIAPEVLGHNYTEKCDIWSCGVILYILLCGYPPFTGKTEQEIFEKVKQGRLKFPNEEWEFISKEAKHLIQKMIQVDVNLRYSASQVLSDPWFQKHATNQPINKKVLDNLSQFQATSEFRTAIVQYIISQMTNHKEIEDLQHTFQSLDINRDGVLSKEELIQGYKRIIKNQEEAEQQAERILEEIDKNLSGQIDYSEFIMASINQSKILSQKKIEQAFRIFDLDGDGYITKQEVEDVMGTLNQDVWQLFLQETDHNKDGKISYQEFLKLFSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U171</accession>
    <name>A0A8S1U171_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UvrD-like helicase ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0320267</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8159005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="3156807at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039904">
        <property type="entry name" value="TRANK1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21529">
        <property type="entry name" value="MAMMARY TURMOR VIRUS RECEPTOR HOMOLOG 1, 2 MTVR1, 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21529:SF4">
        <property type="entry name" value="TPR AND ANKYRIN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="465"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2278"/>
            <end position="2312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8159005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2344" mass="277985" checksum="B66FFFC999B06C4A" modified="2022-10-12" version="1">MKQNQQIQQKQKISQTLMNQYLKEKTELENSINQVQKIMNAKFEQKLNPKSILTSYLQTLSHINEYRYMGVTVNIEEDIKLFLQSLPSYTFFPQKIWDFSFTKQFQQKLLSLKDDEFIKVVVIIKILIQGKFNQYQLVNVNQIQVGSIKFPFQYDFQLQLAVDGRTTMFFQVFPKKYPVMNDSEHPIQFDYVHELIFVELNEGTYYKNPQPYEKYLKTQQSIPNFFTDMKYIQEFSDKKKVKYLRVGEINENQFLLIGPRIFKPLLCQGNQFVYNYKLNLWMTIEDEEKYYKKLKQIQEIRQNNQIIKKYCYLPSSQQLLTIIKNIPNFRVKLTQEQQNIISYGGDALVIGRSGTGKTTCALLKLFSTDILYKLRIKLNQIKYSSNDINLSQQDQNSQLKTIFVTASPLLACQVKRLYDQLVNNIQNAINTKRQRVKQEQQENIQTQNIDLEQSTFQIIEALQQNDKDNDDNNNNQQEFQNDNEIEDEDINEFEKEMGKFNKFSEIQQFPVFLTLRKLLALIDSSLIHSFFKVYGGYQNKLSQWHNESSGLMTLDKNQIAQPFNEELLHKHISLIDNQEFIETNLQEVTLEIFERVFWPKIVKELKQEYYDVSTFDPTLVWSEICTKIKGHETSHEYPDKYMNFENYIYYHRILSEVQTKLLYKAFETYERLKQSYGYYDLLDIVNHINYELSQGNDMIECVHYLMLDELQDVPRAVLVLLDRMAEFGLFCCGDNAQNIAKGIGFKFFEVQNCILNYRGNQRRRQSNMTLFDLNINFRSHNQILQLANSVIRVLEICFPYKIDRLKKETSDLTGPKPVILQTEDPQDLLSYIQEFFTNDRKTVEFGCNQAIIVKDQESKEKLPQELQNALVLTIYEAKGLEFDDVILYNFFNDCPTSVDDWKILNEFEIQSVYMSEEGYKNYLTVHQSEVVTTDYNDQKKLVEIKQLKLKERIDLKKQKPNLDAFQEYMSLCQDLKQLYVAITRPKKRLIIFDQSNKKRFIIQSIWEKLDVVNIVQKKNIQVSDTQFLLEHTIDNQANWKKQGYKMFRLNNYDQAAKCFKFSGDEELAIKSRAYFLATQANIFNENKTNYIAAAKLFEQINFPLRAAQCYFSGKEYAKAYEQYKLTNCKSETAESAYFAGYFEEAADLFYQINDIRRALDCFKKANNWQRIIELLNLHKDEFTIDERMAFLNKFFPSFLQEMTDQIEQQEKEMEEQNLIEETSIIQEDINDQSQSFQIQNSLINESQQVQEENADNNIETSQISILKQNKQDIIEIDDSESFQVQNEESFDHLSIFDPEDEWLKNNQKSLIKSIATSSVESQFSNVLLLNQPSNVSLLKSRSNIFIKNNVLQQLVQKFQFFSDEFKIHIEKQTHKATLLSNRTGEEKEFDHMINFLYDLDNIDIDTIYLILDLLEQFKSYKLCIYVCNQFKLAQHIGRYLVSLASLYTPLTRNPLKDNYQIIANKLYRKQILQQAAVSQLAINNILESINPKFLQFKHEEQLNSSNSFGITCYKELIGLGYWKTVIYQLNYDHAYNLCRSFNSYQDIFNIIEKVKENRKNKILNKQEEFNLNKYQYFYAIQEALQLNNSNFNSIFSTTINYFNNKQQVNQEIIKDIIINSQLKNTSNLNYTQQLNQIESIILSHLILKRNNTSQEKQKLVDITQLVEIQIYFLNKMQLFCKNSNIIDSFGFLYQFSLPKGEIMDHYSQYALVYLTSDLVSYLHSSSKEIQKDPSQITQQELKFIDIGFEYILIPYNLILKTIHKSFIQNLSNQFYKMSEQLKSFYLTYGNQQQQFPQNLYRDSYLPIFQQLYYLELRQKNILPIIFLPKQINFQKFKNFNDASIKNLEIDRHSSSILNQIKSLHQLDFFKILIKTNIRNPNIPSSIKQFFINESISLLQKSQDQLSHQKIVLAINILNYINELPLAIFTLQQMKKPIQTNPYIKYMEFLECQEYNITEDLFVCFAEFSVDLQNKLFLDEWLYHLIRVGLSILISILEEKKQKIYIPQIFVEFVKGIQGHQIESPIFKIQNDEILLEYLELLGQFLDNCNSEHYEYYGNILLIIFVINLPILTNEIKEQISKIIEFDSKYKFYQRLKVIILKDLQVRQNEYIKQQKSIYINQYLDLQITHLQIINTKQQNQLEQIYNTCLQNWENYQKKSEQIKSNGQNLIKKWLIHKQFIAKSKYLSKALPKFQLQNQIVKFQQFYQLNLKEFYKNLNNKDIKLENNIKDAFAIQEDILNQRHGAVDSIDLHFCNLLLEKLSELNIQILKGNNVSQQLIELSKELVAWKENINSAQDKQDELLARNKELLAIKWRNLKSGIKLKKRVLKKVEMQTIQEEEEEQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U6P1</accession>
    <name>A0A8S1U6P1_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trichocyst matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PPENT_87.1.T0330311</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000033">
        <property type="protein sequence ID" value="CAD8159763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U6P1"/>
    <dbReference type="OrthoDB" id="298080at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035922692" description="Trichocyst matrix protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="259"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD8159763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="361" mass="41186" checksum="8C21450101B65562" modified="2022-10-12" version="1">MKVVLVLLISLALSKMATDPKVVLAEIDGNHMGNTFLNAIQISLATGSPVHEIQSYINNIRFMLEQEQKDSDLYIQNTQASCNRLLHDFSTNLAYHQSQLKAHSKIVDENTNNLQRSLNKIAEVSVEIEENSKKTNAGQSERDLQYAEFQSKIKDHTEAIAAIDEAYALIEHLSGGSSFIQVKGRFNKVLSRLQSQSTSSGLLFQPILTMMTQLSAKSDSDTAKKVLQLLSNLRVQIVESKSNDEDIEKQQSLNWQQFLSDLTNERNTLSDQRQNLEQAILNYQSIIEESQGKVEYHAAEVERNQSNLEGQDQWCRQQQDIYQMETQSRVQTQDLISRISDHIQDKIVTLKEYLRERLQLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1U7L6</accession>
    <name>A0A8S1U7L6_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PPENT_87.1.T0320080</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000032">
        <property type="protein sequence ID" value="CAD8158666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1U7L6"/>
    <dbReference type="OrthoDB" id="4139357at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000457">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF5">
        <property type="entry name" value="MAJOR FACILITATOR-TYPE TRANSPORTER HXNZ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="301"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="334"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="419"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="21"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAD8158666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="454" mass="52118" checksum="886887B6D12D0101" modified="2022-10-12" version="1">MISFDDLLENKIQTGYHQFKTLGIIGLVEFCDGIEYAYMSILIAIVQKEWDLNKQQTASLGSIFLLGIVIGNCLCAFIADLMGRKTTFTIFTGLSVILIYYTSICNSFNEMMILRLFFGIVFGTSYPLGYVFITEVSEPKYRGRFAYTMGLLFVIGKIYLAILCFFYLDDYTSGNWRGLIRVNGIPVALTFIFSLLFLKETVRYYLNRGKYQIAYQEIEKILQENTGNQETLTDDEKNGLVIWQEKQNEINLEQQINKYGILSKEYRFITMKIWILYILTNMQNMSIYLLMPFLFTDNNSGLSSMLYMFIVELIFAISLYYFIDDPNIGGRKKVIGYSAILLIIANGLLYLLRQQFLFTGLLLIKLATRALFSTLGLVCCESYPLSLRSQGTAMAYGIGKSSAIPSPFFLFPLFYIDPYLPFAVMCLFSIIMLIIDCFIENDKTMKPLESLKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UEL9</accession>
    <name>A0A8S1UEL9_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0390141</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000039">
        <property type="protein sequence ID" value="CAD8163280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UEL9"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004771">
        <property type="term" value="F:sterol ester esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002168">
        <property type="entry name" value="Lipase_GDXG_HIS_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23025:SF3">
        <property type="entry name" value="HORMONE-SENSITIVE LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23025">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01173">
        <property type="entry name" value="LIPASE_GDXG_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="3">
        <location>
            <begin position="352"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8163280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="590" mass="68471" checksum="574B623CC50A6F21" modified="2022-10-12" version="1">MKKVQHSEGMIDAIYYLQKCQLIMKHFNTQNPNVIEWLFQLDETQKQFEQNQCHHPLLEQCAQFINHKIIHPFLLCLEQVLQFKNFEQELQKHKLEIDYVIRRLQIIREFLPNISKVPLDDIYCLSNTHQEWAHLAKYIQFIDLDDPEILDLQFEKFNDYISVGCAYAKLGNMIKLPVFSMIFQNLGGAFLITNIEKARQIKLKEMTNPSVKFIQNLWSIQDKAPVLKNFMKYSMTKIQSHHKIYVPYLFKPTFTLERINQEIESGTLFQQEEFKGFDLIDENLYEKLIQSLFRKDRQNKIKVRVLSANKEVFRSKIDEELIVKQIANISKDHNNIFEGTSPGLDGKIKKVILQIHGGGWVGMSSFSHQTYTRKWANHIGEDCVIFSIDYRLAPKYPYPYALEDVCKFYMNINLEQLILAGDSAGGNMALGICFRAIKMGIRVPDGLFLPYPVVNLNFKVFNPYLLNGLIDQIAPTTILVVVLREYLNSGKEHPDTDPYLSPIIADDSFLTKLPKMRIICGAKDSLTGDTIRFVNKMRKLHHDIKLDIFNCLSHGFMNFEVPIVGVTHIGSVINISCKMVQELFKSSEVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UK11</accession>
    <name>A0A8S1UK11_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PPENT_87.1.T0420198</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000042">
        <property type="protein sequence ID" value="CAD8165408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UK11"/>
    <dbReference type="OrthoDB" id="293479at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAD8165408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="267" mass="31472" checksum="21479B352CB8E333" modified="2022-10-12" version="1">MQDKSNTNSSVIESQSPKKGVESFEQQKRRRTRKVSDALREYKCECGKSYLSYPALYTHNKQKHNGQSLKNLDEQRQESTFSESQTQYIFATLANALGKQEDQQQTSLTVQDMYDLVMWEIQLYMMESDSKEFRLDYYHELLKQSSPVEVSTPQHAFIKFIHEVKEFLTFEQMKLVFLFVYTFRRLLQENPDCTTNNLVEKANQLICSQLEQVLKSIQQLSERHQIVHLSRMGNLRETMIQIVLSFCNWLYARNLTDDKLSLKSDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UQ12</accession>
    <name>A0A8S1UQ12_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0460120</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8166891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UQ12"/>
    <dbReference type="OrthoDB" id="10248487at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21459">
        <property type="entry name" value="DLC-like_TCTEX1D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005334">
        <property type="entry name" value="Tctex-1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21255:SF7">
        <property type="entry name" value="DYNEIN LIGHT CHAIN TCTEX-TYPE PROTEIN 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21255">
        <property type="entry name" value="T-COMPLEX-ASSOCIATED-TESTIS-EXPRESSED 1/ DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03645">
        <property type="entry name" value="Tctex-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8166891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="123" mass="14137" checksum="830692E82F81C4BD" modified="2022-10-12" version="1">MDSGGANTFVVEPKDEEKFYPSKVRKVIQEIMDDKLKSETYDANNTPILAEELVKRIRSKIRDSIKMPRFKIAVQVVIGEVKGQGCQVTSKNLWDPTWDNYVSHIFQNETIYGVAIVFGVYYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UT80</accession>
    <name>A0A8S1UT80_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RCC1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PPENT_87.1.T0430065</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000043">
        <property type="protein sequence ID" value="CAD8165666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UT80"/>
    <dbReference type="OrthoDB" id="10256179at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051553">
        <property type="entry name" value="Ran_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982:SF1">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="30"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="79"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="RCC1-like" evidence="4">
        <location>
            <begin position="103"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="131"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="198"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="249"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="299"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="348"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="355"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAD8165666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="401" mass="44066" checksum="BE8380E9E8FFC438" modified="2022-10-12" version="1">MWRRALQNCLKIAFIGSSITFTSSFVFSQEQLYAWGWNNFGQLGIGNSISQYSPQLVPIQNVLFVACKSSVSAAIDKSGKLYTWGRSKFGLLGNGQVENINVPTLVECLKHLDFKYVACGNYHMAAITNDGQLYTWGNFDHGKLGHSFDNSVKLPSREKYEYQKNLGSAKLPELVQLNFKVRQVSLGDQFTIILSDEGNVYSVGLNKKGILGYETKNAEELNFKQITSLKNIVQIDSGTDFSVALDSDGNMFAWGSNYFGQLGTPSPSVVQIPQQIKGLPKIKQFSCGEQFIGALSTEGVLYTWGFGGDGQLGTPSKQDIPTPHKIQFDHNIDKVSCGQAHVGIISNNKLYMHGRGKEGQLGRGSETESPNSSRYSPLLVMENVIKIACGGSHSFAIVNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UTB0</accession>
    <name>A0A8S1UTB0_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0460089</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000046">
        <property type="protein sequence ID" value="CAD8166829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UTB0"/>
    <dbReference type="OrthoDB" id="310850at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="72"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8166829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="345" mass="42218" checksum="656E8571A5638BE8" modified="2022-10-12" version="1">MEHQQYIEKITEERRIRDEQFLRRKLRLLEEEQIKQKSLQQLETIKLLQLKELQDSRRLQSKLNMSFIQLKSDERTQKVKEQNKRVKQLLEQYKKEKQQQEIQQKQLEQEKEQEVKLKLIQIKEKFKLLTTEVIKEHQYKYKKYQQEHQQQLTILRDQKKLQDTYFSQQVQSRLRSRTYQAQIEMMKEEEQKRKKNNDRLRRQIKERFAYDHFVKEHFLPKIFHSIDLTSQTIINQKPNNQMSPIKSDSRVLGNLYLRQIRKLPRRPRIQSQIAADQSSSSTIKRDYLTEIRQHSKKKTPQTPIDIKFQADRLERESKQAYTKSKLKGDQLLMESIKQKMILLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UTS7</accession>
    <name>A0A8S1UTS7_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0480071</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000048">
        <property type="protein sequence ID" value="CAD8167884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UTS7"/>
    <dbReference type="OrthoDB" id="309259at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8167884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="321" mass="37290" checksum="057208D6315981F4" modified="2022-10-12" version="1">MEIFLNGYPCIVPEYFTFDQLFQQTGQPGNFNNKNWQRNGLAILVNQKTSVKDFLQKNDQVIVFDKPQKLGSTIQSQQIGTFTGAQFVSPSFQMNQGTQQQKIIFLQNQNVPNQQFGMQQVGMQQVGMQQVGMQSMNVMTQGQQDYQLPQYNIQTNNNLLQNQNLYQQPIISQPQPQSQSDDYDVFQTFTDNNQNANEDIFSTHYQYSNYEQNRQKYLQTKSTCPGNKQDQVIKDNYRFTLDNYGDVLIIDKLDQSFYLQISNNFKLNKVRQFDIGNKQKQFESNIILDLNCTRVSWIQDLDLLAVQFNTGAIIAVQLYLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UVA3</accession>
    <name>A0A8S1UVA3_9CILI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PPENT_87.1.T0500105</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8169010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UVA3"/>
    <dbReference type="OrthoDB" id="311554at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAD8169010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="210" mass="24368" checksum="0A46960F59295512" modified="2022-10-12" version="1">MFSKPQILFGCDSYFNSPVVSYCNSPLRSENEYRWMSESDQYPIYPAKKEIIVQESEENFEVCSGTTKIKNLYSGKMKPSIDTALEKISAQQEKEIPQIKKLISKKEVHMLPGESKNIPKNYTRALKQFIISNFDSSVLQNADIKKFLATKPEKACKQTLENSLKNCQQLKQISQIFFGNLQWGKTFLEENKVELEPYFRFNSIWFGSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UY46</accession>
    <name>A0A8S1UY46_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Alpha-type protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PPENT_87.1.T0500003</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8168812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UY46"/>
    <dbReference type="OrthoDB" id="301415at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04515">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004166">
        <property type="entry name" value="a-kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056861">
        <property type="entry name" value="HMCN1-like_VWA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052969">
        <property type="entry name" value="Thr-specific_kinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47763">
        <property type="entry name" value="ALPHA-PROTEIN KINASE VWKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47763:SF5">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_25, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02816">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25106">
        <property type="entry name" value="VWA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00811">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51158">
        <property type="entry name" value="ALPHA_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="VWFA" evidence="7">
        <location>
            <begin position="241"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-type protein kinase" evidence="8">
        <location>
            <begin position="469"/>
            <end position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAD8168812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="888" mass="105267" checksum="26FB5928BD5B90F7" modified="2022-10-12" version="1">MDFLDDIQKLCHDLHKCQIQNCEKLHPKCFSGVCIGFLQGMCQQQDYCGLYHFSPDELQEQLEKKSINGIYPCNLCYKNCNNDQNCKYLHPPWLQYICVNCKNKKCDKKKEGLWHTNRWDEIQKKVYELYNINKLNPEIFCKEVECQCDAKKYNFHKYCIKYFQGTCPYHRCQKPHKNWEDLKDIGNFQNLQDEVIKPCIKFDQKVNKFVSQPQTLQCNVFMQKNQLKLMINQIQQRPQVDIIFILDCTKSMDKWIEVSRKNITSIITEFKKKVQIDTAIRMAAVCYKDFSEGPNHIKYHNFTVRPEEIEKFISSIKAEGGDDIPEDLQGALDVAYKLNICKDPESLLQLFIITDAPCHGRKYHKLVYDSNLNAQDLEEKLQKFVELKQRFFLSFLQINEQTQIMEKVMQSVVQKNYQSAKIADKQFSDYILFSLSSTYCKSQSITNIKEYQHISQAQFRKQKQMHYAYHNRQNNKYYDQIIEQIKQTQKKCQTLLFIENDELQLKQKEKGQAAEVFKGFDQKNNVYVVIKIPRWILKKYNSQTLTDDDIDEANKIADIKYKQQLIAKQLSNHFNYCCKQQKVQFIPLYYATPYLYELEKPFKGLKLLYAESYIDINEPWKKYTNNADYRDEEINLTAFSHFTYQYTNFQMIITDLQGKINILSDPAIHTQDLMDSTNQQDDGCRKFFLNQHPECSNICKKLGLGQTIIQTKSLTFIEGYNSEVDTQVEDVFGNKINREKLFKVCNNCNALEKFDEASNNYELCEYCQEQQYEKQSMNCKCCNATFSMSYNFEQRFGTTVQYCQECQNNYCHQTLEKRCYYCGKRICLQTIKEIIVNKQKLDICIDAFFFLRQIKCKNCHSNYNFDTLLSQEDYYGNNYNCGCNKINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1UZV8</accession>
    <name>A0A8S1UZV8_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Histone chaperone RTT106/FACT complex subunit SPT16-like middle domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PPENT_87.1.T0530160</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SSRP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000053">
        <property type="protein sequence ID" value="CAD8170640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1UZV8"/>
    <dbReference type="OrthoDB" id="498543at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035101">
        <property type="term" value="C:FACT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13230">
        <property type="entry name" value="PH1_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13231">
        <property type="entry name" value="PH2_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.150:FF:000001">
        <property type="entry name" value="Fact complex subunit ssrp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.220:FF:000004">
        <property type="entry name" value="FACT complex subunit SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013719">
        <property type="entry name" value="RTT106/SPT16-like_middle_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050454">
        <property type="entry name" value="RTT106/SSRP1_HistChap/FACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048993">
        <property type="entry name" value="SSRP1-like_PH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024954">
        <property type="entry name" value="SSRP1_DD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849:SF1">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21103">
        <property type="entry name" value="PH1_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08512">
        <property type="entry name" value="Rttp106-like_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03531">
        <property type="entry name" value="SSrecog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01287">
        <property type="entry name" value="Rtt106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone chaperone RTT106/FACT complex subunit SPT16-like middle" evidence="11">
        <location>
            <begin position="272"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="76"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="369"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="76"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="96"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="373"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="400"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAD8170640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="434" mass="50235" checksum="BADCA8573D252DBB" modified="2022-10-12" version="1">MSDQCTKGTNWGVVSIDDKNLCMKYNQSNIIKLPLKKVVNSNTQKNDIVLQLSTDDYGDNDDMLCEVRFYIPPQEQKLKQDKEKKKQENEDNQNSMEEEEDEADEDAEPTFQQKLQNEILTKAKIGQSSADSILTIHDVPLIVPRGKYTMDFFKKDIRFHGNTYQFTTDYKGISRFFLLPMPDEINLSFVIGLENPFKQGQTTYNFLVMQFKKDFENEIKLKYSRQKLDEIGWKGIKEEYSGPLYDIVCEVLSEITGIKVVSPKNFKSKNGLFCLRCSVGPHSGFLFPLEKSLIYLQKPVLHIKHEEIKEVIFQRIGSTNLNKFFDVKIVYKNQNQLFSGIERDELDNLTQYFQQKKIAVRKLQDEVPHLPLDDSDDEEDDDDSRQKKKNKTNIDPNNMDSDDDDDDFHAGDDDEDGSESEGSDEPESASRNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V0E0</accession>
    <name>A0A8S1V0E0_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD domain, G-beta repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0500231</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000050">
        <property type="protein sequence ID" value="CAD8169239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V0E0"/>
    <dbReference type="OrthoDB" id="406844at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="313"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="366"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8169239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="594" mass="69375" checksum="9BE5B1C843D445B9" modified="2022-10-12" version="1">MFMPKMIENILELQCHQKHSKPISAIRLGQYISNEKRLYCEECSKLNDIQIIPLTLAVELSKDSIEKKLRKFQNLFVPLYDLLLKLQSHIFELKSQLFKNFHELEIITEDWINFIQVSEQQITRYSLFDELDQLIINQKQTQYQIDEKIINDYKNVNLQQLTKVNSTLVQFKDFTNKFRDCFKIIIEISKQHKESQLKSKINFQDNQSLSDQLVFQKSSYFQSKKSIPNVTSLEFSKKVTENMLQFQFKLLQQSFKQQEECNAFAINHNNTILLAALNNSPLFSIKIIQMSQQQEKQENKDASLNLKVIQILEGNHDNFVTTLNFFSSTPMRDSFISGSSDTTIIIWSPTTKQNNLKLFWNPLIKLFGHQSIILCLVLHHEENLFISGGSDNKIKFWTSSIQQQWFCKQTVEEHSDKVYGLSLNDSGNQLISCGNDKQILVIQNSNNKEWDVKQKIKILNIGFRISFITNKLFAFQPSSQAYLHFYELNSFGEFVNTEGLQVKGVDQCCDCFFPSVYIPLKGIMLLKNGQNINIIQFSFFSQNWGCQLIQAIEFETQKFLQGRIYGTISADGHFIVTWDVQSKQIQIRQQRMMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V515</accession>
    <name>A0A8S1V515_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Arrestin C-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PPENT_87.1.T0550272</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V515"/>
    <dbReference type="OrthoDB" id="2333384at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF17">
        <property type="entry name" value="FI21816P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="1">
        <location>
            <begin position="208"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAD8171905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000689195"/>
        </source>
    </evidence>
    <sequence length="408" mass="47154" checksum="FD2AD3541BA9E42D" modified="2022-10-12" version="1">MNKNVIFIFKSPVTLAGDPINGQCCIRITQELPKCLISLSFYAKQYSKLIQKRKLPNVTPNSIPEKELEKIERIQVSIDSQQKTQKQKKYELNGNVIYRIIRQYGLQKFLLSSQELFKGQVNPGDYKFFFSIPTKENMTSSFFYTSQDGLKSGKCSYKITLQIENPDECLNILKESAEVYINARVQSSVEIQRQSEANIISYFCLNGGIAYLSIKLNKSNFTPGEQAIIEYQIDNSFSSKNVNRVEIRLINKLLFIDDDQVDRLVLNQVVFSKILSGVNQGQRLEAQIAQFEIPKNLKASVRNQTISNQYFLQIEALILIKSNPLPNTNQHLRISISIGCQSRWMEFRSNFKLQCQCFFQSNCSTIVYSNMNIFNQFIYLYGQNYIAIFSFNTISGRLFNTHSYQFYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-10-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8S1V5I4</accession>
    <name>A0A8S1V5I4_9CILI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serine/threonine-protein kinase RIO1</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Serine/threonine-protein kinase rio1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">PPENT_87.1.T0550258</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Paramecium pentaurelia</name>
        <dbReference type="NCBI Taxonomy" id="43138"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Sar</taxon>
            <taxon>Alveolata</taxon>
            <taxon>Ciliophora</taxon>
            <taxon>Intramacronucleata</taxon>
            <taxon>Oligohymenophorea</taxon>
            <taxon>Peniculida</taxon>
            <taxon>Parameciidae</taxon>
            <taxon>Paramecium</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2021-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Genoscope - CEA"/>
                <person name="William W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17 24">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 24">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="CAJJDO010000055">
        <property type="protein sequence ID" value="CAD8171877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8S1V5I4"/>
    <dbReference type="OrthoDB" id="205248at2759"/>
    <dbReference type="Proteomes" id="UP000689195">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05147">
        <property type="entry name" value="RIO1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000148">
        <property type="entry name" value="Serine/threonine-protein kinase RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051272">
        <property type="entry name" value="RIO-type_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018934">
        <property type="entry name" value="RIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018935">
        <property type="entry name" value="RIO_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017407">
        <property type="entry name" value="Ser/Thr_kinase_Rio1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45723">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01163">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbRe
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